LOCUS       DAEORZ010000001        66737 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEORZ010000001 DAEORZ010000000
VERSION     DAEORZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 66737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.001"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..178)
                     /locus_tag="KRH39_000001"
     CDS             complement(<1..178)
                     /locus_tag="KRH39_000001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509936.1"
                     /translation="MKENKILQRGRYLDTDIYFDEFNKDFDNYDFVDIVISPGSGYTF
                     GIKIDRSIWACGYNV"
     gene            complement(194..2263)
                     /locus_tag="KRH39_000002"
     CDS             complement(194..2263)
                     /locus_tag="KRH39_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG8509937.1"
                     /translation="MKNYEIKNHTIEVDFSNYRINKRLLDYFIYNENDVKTAYIEAIL
                     KNKDEIIDLSEYDRVLVSITKSDGQKVNGECEVVDAENGIVEIELSRQALASVGINTF
                     QLSLVKEGTLLNTTNLYYRVEEGMINDDDLTSTDEYGVLLVLISQAEEIIKNNKELTK
                     RVEQLEITILGNEEVRDKAEQIRIYNEDIRNIQEEEREFNELTRQNQEANREESIQNM
                     QIQVDDKLTDCQLQLDEMIDAKSEEIDHIVDDKMLDVQAQTDKKFQDLDTRANNTFDL
                     YDKTFEDKLTDNQEQIDYKLDEVNQAISNVEDCIDESTTKLDTKIKEVDDKIVEVNTA
                     KTDMTTTVSNKITEFENRFEELESLDARGELMQSRETIDGTVKDTLKDRLTYDFEKVN
                     EKIAEMTSAATNVAFSKSYVESDWVTNGEYFKLIVNHNLVTENIFVAILDEATKKSMT
                     NSYTIIDNNTIEIFNESSINVRVTVVNGNTNKEVIPNTINDNITTLDSTYSSVKIDAK
                     FDESLIKINENKSNIATNLEKINLIQNKIGSSELSTLSKNISDAVNELDASVKILEAG
                     GNLGQKLDSLKAKYDALSDKVLDILIYLELESGAVDEVGQWYDNLADSKNILSIDNLR
                     LDTDRRRIFGSEGNVIFNKIDIPFSCYKVKYIHELDDNFIETISNTSTNAGANSIELE
                     KFSYEVK"
     gene            complement(2275..2436)
                     /locus_tag="KRH39_000003"
     CDS             complement(2275..2436)
                     /locus_tag="KRH39_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509938.1"
                     /translation="MEVNMQRAYTIALEEIRGLQNELILYKAFNEQLQEEINKLKKEK
                     VETKQNKEQ"
     gene            complement(2452..2757)
                     /locus_tag="KRH39_000004"
     CDS             complement(2452..2757)
                     /locus_tag="KRH39_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509939.1"
                     /translation="MAIGKNKLLATGQQVYYWRISTVNISYSNKMAQIKVSGYISEDA
                     RREGLDAVEYDNISVLEDNFDNYFGINTLDATGKNPLQSAYNYLKENIEKFKDSLDI"
     gene            complement(2738..4663)
                     /locus_tag="KRH39_000005"
     CDS             complement(2738..4663)
                     /locus_tag="KRH39_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HBG8509940.1"
                     /translation="MILDNSPADSWIRITGVIVTYNNTLYQVVDKETNKKYIYWDADN
                     PGTLKVSNVRLPEGNTQFLVVVNDNGKHTEVPINSSIFNISFDGNSRKNTEEQIWALY
                     ETDEKHNEKFVVIEKDIDGIHQTVLEVQEDASHIKENMSLIDQRAENVNILVKEVTKN
                     FGGSQENITLRENINKAIIKLNTDLGTFSSNLSNYFNDNEITDEEKDKIDIELNLLDT
                     DKASLYAELQKLIDKTTGVDLVAINTSKTALDAASTNLNSIINSVISDSIITPSDRIL
                     AINANAQYNLKINELKNTVDKIYITGMGGSISEEFSQINATAKEIKLEVARVDGATKT
                     NAAEIKLTKDDISMIVTRNSDKAIVGIQSDKIQFGFNDISDYVVINRSGLIVNRGSLA
                     CNAITSVDDDNAIISFFSDSKNSFAIDARPSDGYGKRSAFRFKYNSSNYFFVESGKAA
                     IYVNAYENYIFGSNNAKFGANKIDVGDDGYTRMDLTGGNFRLYTSEDWDDDNGIVIWK
                     RGAISFMINGEEKQQFARNGSKSAGSIELNGTTFGMSPIDSPKFLIEDILFDIEVEEI
                     GTIIKLNNIFAQSILNFAVFSSNSNCRVVEKGEDYFKVVGYTGKCDFRVIGKRIGYEH
                     TYYKIMGGEEQHGDWKE"
     gene            complement(4665..6380)
                     /locus_tag="KRH39_000006"
     CDS             complement(4665..6380)
                     /locus_tag="KRH39_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509941.1"
                     /translation="MHFNVNLKQIKSDYTLTIHKMNKSFLGQIPINFLNAIKRELGGV
                     DEIQLTIPKYITDRFLFNKIINPVFEEVKEERLICLNDKEYFVVKNVVTTDDKLKVVT
                     AKSKEVKLGKIDVNIEDYGLQMFTKDEETSIISLNDYLKQETGWKLGHVDDSIAYETD
                     SEGNKREKVRWQESINSNWLDYFNNELKEQFECIADFDTYNNLVNLYHIDSFGDNIQL
                     YLSHDNYIKSLERTTNSDDIVTRLKLEGSEDMDIIGATVTGYDYIENYSYFLDNKEMS
                     EELSRAIKKYQEMNEIREPIWRELIDTKLKKQRERDSKSNEWLHVIETIKAKKDIKKT
                     YDNPEHKDEVNSAKLAVEISELEDKKVILDVQIKHLEEEIAKLNESIKDINILCKRET
                     STDEDGYLIFNEALLDELNEFLYYDTYTNDAFLKVEDLIAEGKRQLSLKCIPTREWTL
                     DVINFLDRIIDINFRQHWKGDLSLGDIIVLHSKESGEEELVYFTSFTQNLKNGKLDTL
                     ELTLSNKKIKEDDKRTIADYLTKAEHATRTLNSKRHLFIQQQKKRINLPDEYIPKKNI
                     QKELM"
     gene            complement(6886..7041)
                     /locus_tag="KRH39_000007"
     CDS             complement(6886..7041)
                     /locus_tag="KRH39_000007"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509942.1"
                     /translation="MINNIEDCKLLIETEIRMKGIDEKELISQSEILKDLNINEKDLE
                     DIEVNFE"
     gene            complement(7684..8688)
                     /locus_tag="KRH39_000008"
     CDS             complement(7684..8688)
                     /locus_tag="KRH39_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HBG8509943.1"
                     /translation="MRTDNNKVFCEFCLDDVNYTIKIKEEIDTVRGKEIKYMKKEAFC
                     EECGNYVYIHELNDENLKSLYTKIREMDNIITVDEIELLLEKYNIGKKPLSSLLGWGE
                     GTITRYLKGDVPTKPYSDKLRLILDDTNEMKKILEENKNNIKDIAYKKCREAILEIEE
                     KNTFFKNDKIGVVAEYIISKCEEITPLALQKLLYYSQAFTRLFTNNNLFDDDCEAWVH
                     GPVYRNVYEKYRDFGRNQIEYENNIISLDNDMEEKIVDAVIKYFGCYSGKILERMTHE
                     EKPWVINRQGLDKYESSSNVIEKEVINEYFNNIKSKYSIINISDINDYSSDLFKKIQS
                     "
     gene            complement(8801..9196)
                     /locus_tag="KRH39_000009"
     CDS             complement(8801..9196)
                     /locus_tag="KRH39_000009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509944.1"
                     /translation="MAVSAILEDIEIILKKFKACIESENFIISQSENRQDNNDFLKKY
                     NLDKNKQIEMLNCLTKEDFSKSEEHRNHEGRILYFFGKKYEFTNIDGLREEVELYIKF
                     YIAEKKRTKEDFTIIISFHPAKYSIKYYM"
     gene            complement(9411..15251)
                     /locus_tag="KRH39_000010"
     CDS             complement(9411..15251)
                     /locus_tag="KRH39_000010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509945.1"
                     /translation="MIYGMLDGLINLSEGIDKVIKSLDKMDMLLPATITSFAMLKKLR
                     EQSKVKKNQTTTINNNADKNTDESIKKVSKLATSYNKVKQEVNGAIGAMNKLKVAGRT
                     SITEISNLGKSMGSKFKNFATSIGSSLLSGFAIGAIISGITMLIQKYDEWNRRYEISA
                     QKHRENIENTKSEISTLSQQQQSLKALSEEYNKLYNKTNKTSDELERFKQLQKQIADI
                     SPDLVAGYDEEGNPILAFSGSLDEAIGKLQLAIKEKNELLKTERYNTGLNAMNLAPKQ
                     TEDIDKLNDKYIEKLSDNKNDLDKAFGKGDIPFKTKAIEKSLKEYKKAYEKRNNLISE
                     KNQEAKELMDEYNLAEAEIQQVSFDKIAEKSNYKNATNNVKSQINELMDVFNWHDISV
                     TEQDVLVSGFDRLSSIISDNDPKLAQWNKRLQEANTNYKAGGDAVEYANAVDAVGREI
                     NKTLVAAGKEPIDTKLLEKGLQNLRGESTATAEALHAFLTSYDSSTAELENENGIAIK
                     LKSQFDDLNNFISETINDPDGKIDISSLIKINEGKTHTNLPKQVKEMIRGILGKNNNA
                     TEFEQQILMKVSTTIQNEGKLPDYVANQLNDIFNGKVNPKEIETPIKIGENQLISKEE
                     LKQLAEYYYGNEENRPKIPIDFKTDRKELEKEAKEAKKTLQDILDKNENKSDDDKKNI
                     TKDSLKKAIESKGISGSVESLNALNSALEKVPAEKKIDFINNSGDAFQGVKSLDEAIK
                     KIPENKRLQYNVGVEGNEKLEKLKELYDKLPESIQGKINDGSININKIGNIKNLLDGL
                     NKKDRKVAIDFIVKNPEQLDSFEKFYNGISDSKSKKLALEFATENPDKLQDGIKLYDS
                     LKDGNQKKQILKFMVENPEKIQSATELYNNTPEGQKDLIYNFIVNNPGEINKVNQLIE
                     KYPDKKTAILKMAIANPSVLEDIKNVNNLPDNIKKMLGIEVKGKEDVEETKKDLDEAK
                     GNHNAELTVKTEGKEEAEETIDKVQNGDGTNAELLVSAKVQGLENGEVPGFDFENNEP
                     CILKVKPEVTNKEELDSTKQKLDELNGKTATPTVDVKTEGKEEAEQTAEFIQGISPIK
                     VVELSLNNEGKDELDNTKQKMDELNDEKVEPVVGAIGLEDTASKIASLRQQAEEFGQN
                     NYNINLNAETSQGAKNISGLIARVKQYLALKVSTLVFRTETAQGAKNVSGLLAKVQSY
                     VSRYGGKTIKTTFSALTSTAAKNISGLIRKIESFKSNYAGKTFTTTFVTNKVTNSSGG
                     GGGGNSSDKTNKTERTSVTNGTPIPANLLAQPRTSEPAPISDETPVTKPSLFSRAVSR
                     ATSAFKVIKPKVTINNDTIDSSVKYSIELFKELENAISAVTNEIALLDKKMEHATGDE
                     KIKYLQEQNKLFAQQKDLIKQQQDGYKAESNRLKNRLKEKKMGFKFDDTGNLTNYEEK
                     LISIEKELDKLNSKSGDKENKKDESRKKELEETKKVLEEYIKVTFTELPKCEQEWEEY
                     NNKIKEHNEELRKAQEEMWKLNKNSTWTSMYKDVEQVNNEIAMIDVLLKNATGDEQIE
                     LLEKKKELMAKKAKELSETTAYLQETQKELQQKLQGYGFTFRDNGDITNFIQHKNQLK
                     KNMTDEEFKRMEDAAEGYLDLLINKIPDANKEIAESKKEIEALNKEIQDAYKDQLKEA
                     QSLQEKIRDMYKKELEERLKEIDKETKAKIDSLKKQQDAYNDSRKEAKYQDDYEEQQD
                     VISDLEKQIAIAERDSSLSGQKKLKDLQKQLKEEQKKLQDLVQDHVDDQVNDMYDKES
                     DRLQEEADKLKEELEKKYSDENLVDLINEAISSGKFVGLDGEVKKLQDAIIEYINKYE
                     DGMLAMGSVTKQEWLDKLKEGKDTLEDINDILDELDLSKFAMPNYNPSSNSRSRSVSP
                     TSSVNFNSPFFVVQGNVTKDTMKDLEKFGRDLENRVYRKIVENIKS"
     gene            complement(15950..16135)
                     /locus_tag="KRH39_000011"
     CDS             complement(15950..16135)
                     /locus_tag="KRH39_000011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509946.1"
                     /translation="MGLKEIAGITIYKNKDTGELKNMVEVLDEVKSKWDELREDEQKG
                     LAEAIAGKLFAVLSSDI"
     gene            complement(18106..18261)
                     /locus_tag="KRH39_000012"
     CDS             complement(18106..18261)
                     /locus_tag="KRH39_000012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509947.1"
                     /translation="MDVESASSAVNTIINGFRIEPLKEMQVQVGNTTKKTTELTAAMD
                     MLNCLAC"
     gene            complement(18833..20341)
                     /locus_tag="KRH39_000013"
     CDS             complement(18833..20341)
                     /locus_tag="KRH39_000013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBG8509948.1"
                     /translation="MSEFKIKTSIELDDEKAKAQLKSLQTSAKENNIKLNVEMNTSSL
                     NNLKQLETVLSQINKLSRETQNGLFGGKSTNGSNSGVNSLLSEYQKLINTKNSIEKQM
                     SKTTNVQSYAVLEKQLSSVLGKIQTVGSALEGLQGKAKIDSSSYENMQRTLASTFQKI
                     QGDIQKAQNQIAKFKQTHILDEKQSAIIDSVSKQLNNLSNIKLENLGFRGISKLLADF
                     QSVQSVMANLKIPEVNSKVESQFNSMQKSVDGFINKLKQLSGNKYADKNVLGGLLSQA
                     EKLKSLDLKNLTGDELKKAIADLENLELKLKEVQSATNVSKTKSEFQFNFDKAISDLK
                     RLKQSALELGQSTSGIEKLEKELMQLANLPLDQKVSKLEQIQRAINNMNKSTTALSSS
                     GKGVNKFFNDFTRSFSYFTLGNMAAMQLQNAIRGVVSTYKELDKTMTDLKKVADPVDI
                     NSVQKLDEIRSKANQTAKEVGMTTSQVQESIAASLQAGIGGIFMPHYIEIYN"
     gene            complement(20405..20971)
                     /locus_tag="KRH39_000014"
     CDS             complement(20405..20971)
                     /locus_tag="KRH39_000014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509949.1"
                     /translation="MYKKILLIGIVSVLGLGFVGCSDDLSEKELAKCEKEWNNLEKIT
                     DEIDSEWCEEIFYDIYYENDNMEFKGDITNKRVVKVTFDEEALNKAKETLKEKRKIVN
                     DINIKRLNKYIKYKIKTDDYYKSLSKEEVDKGFEMLSNSKKFLNKGLDIYGDILDKGE
                     KLEYNEEEIKEMKDSNSKVSDLVHENVL"
     gene            complement(21015..22115)
                     /locus_tag="KRH39_000015"
     CDS             complement(21015..22115)
                     /locus_tag="KRH39_000015"
                     /inference="COORDINATES: protein motif:HMM:NF024637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG8509950.1"
                     /translation="MSEDENKVSQENDSTKFCTSCGEKLLEGQLFCSKCGKKVDEKTV
                     ISKKASINKKYIYIFAGIILMVIVGIFTSINIKEKKQQEARAKYIMTIKDFQEKSYVA
                     GANLEDIADTIQTYWKENIYEKKHGSNIDAAIFAAMLDKGDEIEKSKKYDDELLKIYS
                     KLKSVPEGSEDLNDILSTIEKFYNSYTDFHSFATEPSGSYVDYSASNNTKTDEFLSNY
                     RALENLIKSNSELNKAKSTSEIMIEKISKAMKEYKIISDSIPNDIDPLDENLYEKVKK
                     IIPDFYVDKHIDVSDIENNKEKFKNDEKVNNGLEYFTITKTSYDNMIKYANSYKKSNS
                     QDDLKKFKSEMFEFRGYVAMYGVQVESLDEDN"
     gene            complement(22329..23810)
                     /locus_tag="KRH39_000016"
     CDS             complement(22329..23810)
                     /locus_tag="KRH39_000016"
                     /inference="COORDINATES: protein motif:HMM:NF025669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBG8509951.1"
                     /translation="MEGDLGYLPFGEGEIVVKDVKLKKGMRRDEVNRLLKENDVDLEL
                     VDEIYGGNNFNHKWKCECGNIIEKRTWTTVKDKGALRCNKCKYSIIKEKHKIEVEKDS
                     DYEYIDSYFKGDILENGKKSRTVCIKIKHKYCKSIYVVSANAFINMKQRCPKCCGSYE
                     NSFAHYIEKELGEPIEKYWDFEKNKVSPYNIWKNPNSKNSEKEDIRVWIKCIKKDYHE
                     SYLISCTDFVKGNRCPYCRGFKISPKDSFAKYHIDNTDKDFLIKYWDYNKNKINPYKL
                     MPSNKTKIWIICQEKKYHGSYMISCDHFKIGERCPYCSGKKVNFKDSFGYNYFDMAQS
                     WSLSNSISPFKVMQSSGKKYKFICPKCGNEFSRRLADVGEKGCICPKCSKSKGEKRVE
                     QYLINNNIKYKDEVKFKSLVSNLGNPLRYDFYLMDYNILIEYDGEFHFKEYFEGQKFN
                     KNLLHDKMKNDYAKEKNIKLIRIPYWDFDNIEEILDREIRSLL"
     gene            complement(23914..24744)
                     /locus_tag="KRH39_000017"
     CDS             complement(23914..24744)
                     /locus_tag="KRH39_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBG8509952.1"
                     /translation="MSNEKRNEVFTNEQLGTSVRTINFEDGSIGINAEDTAIGFGWCK
                     VERKGEKEYKSVRWKRMNEFSKEFGFDHLWAKDDYIPESLFYMLGMKAKNEAALKFQK
                     WLATDVIPTIRKHGAYLTDTKIEEILSNPDTIIRLATDLKEEREKRKALEVTNNKQQQ
                     IIGELKPKADYTDLILRNKGLVTITQIAKDYGMSGKAMNKKLHELGIQFKQSGQWLLY
                     SDYQAKGYTHSETIDIVKADGTPDIKMNTKWTQKGRLMIYKKLKSIGVLPIIELEEVC
                     "
     gene            25005..25415
                     /locus_tag="KRH39_000018"
     CDS             25005..25415
                     /locus_tag="KRH39_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8509953.1"
                     /translation="MFGERLKELRLKKKLKQSELGKEIGISASTIGMYEQGRRFADQS
                     TLIKLAQYFNVTTDYLLGFSKTNYSVNATIAGLSPIQEDEIITNESINETIKSKQKDV
                     LIKKLYSESQKLNNNELEAIINIINIFNNKKEDD"
     gene            25691..26452
                     /locus_tag="KRH39_000019"
     CDS             25691..26452
                     /locus_tag="KRH39_000019"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024252.1,HMM:NF024806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8509954.1"
                     /translation="MGIGENIKEFRLDKGLTQNDLAKKAGISRVALGNYEREERVPSI
                     EILKKIAIALDTSEEIIMKLQDYTLEEIYRLKIIDKKYVPLTKHQYRFLTEIIDKCTS
                     NILNSASSVDYYDRAYMYIFLNNYEDAIKDYTKAIQLNPKFAFAYLDRGNLYAFLGHK
                     QNAFNDYDKAFEVAPNRDVKCTIFNSSSEVLYKTLGEHIPKNDIDSYILEKKEIYFKK
                     TDSPLANKINLLNDDCIDLISNLVDKLIKDKNNLR"
     gene            complement(27701..28159)
                     /locus_tag="KRH39_000020"
     CDS             complement(27701..28159)
                     /locus_tag="KRH39_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509955.1"
                     /translation="MVFKSLDELVAYTKNQIKTSMPEVGKKMKEIMKEEVDRQVYSGY
                     TPKGVWHPDLWEGRTGQLLEAVDVTELSSNSVTSEIQNNGKWFDAFSKNPAFPMERLE
                     EGKVWGAGGTRKEPNFRPKTNIMTESETKVRTEIPLCFKSTMNGLGVPVE"
     gene            complement(28160..28609)
                     /locus_tag="KRH39_000021"
     CDS             complement(28160..28609)
                     /locus_tag="KRH39_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509956.1"
                     /translation="MGKIQVDKLIQDEVRAIIPIVDESGKEEYIEVRNPDKETKEEIL
                     NKIWIGMENPDLALSQEDILKMLVDKLTNIELNIDIQDVIDGNISSELETVMYYIGQI
                     ENELTASLLMNTEVKLGQLKNEILQDRVIKETEEIEKMNNIKDKVVN"
     gene            complement(28623..29408)
                     /locus_tag="KRH39_000022"
     CDS             complement(28623..29408)
                     /locus_tag="KRH39_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509957.1"
                     /translation="MVFLDDKFLFDNVSSESMNIKLVTLGDDEILNEYGLPYEEAIKS
                     DSSFNKNPCYSEDETTIEPITLQFCLCSEHGEAYEWDDYTLENICNWFWQREFKPFIS
                     YDNIEEIYYFKARKIIKRYTKDKKGVLEIEFQPYTNYAYRSFQKVITVKDTREIKLNN
                     VSNVDEEYAPVIDIECLKEGDITIRNSTISDNEEDSLVISGLGLNEKITIDNLYYTVL
                     NSSGENRFNIVNRKWIRLRRGVNILKFTGNCKVSIKCKYPIIK"
     gene            complement(29505..30320)
                     /locus_tag="KRH39_000023"
     CDS             complement(29505..30320)
                     /locus_tag="KRH39_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509958.1"
                     /translation="MRFSIKDAANAIIVNAITKKPIFYTEDPNTFTFKLESESIYAKA
                     KGANAIAFDGAITSSITIEQEVIQLPQLAMILASDVVEKTAKVGKRELLTSDGAKKVT
                     LKGVKPLQGSLTVHSVEDDGISLIEELQFTSAISGENTEITISSSSFNAGDRVAVFYL
                     TELPKAKTITVNNDSTAPNYTIYADVSVKTAESKLMVLHMTIPNCKAQRSIELGFTAE
                     NPSGFNSTLDVLPDSNGNYVEFAFIGKEDVTPVMIANMLGAELIDEKDTKSKK"
     gene            complement(30366..31166)
                     /locus_tag="KRH39_000024"
     CDS             complement(30366..31166)
                     /locus_tag="KRH39_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509959.1"
                     /translation="MLENYYITGLPIKLSEILGTVYQPTIEELIKLDIANLEIVNPFL
                     ILEKNYSQLCNNEFFNLKYKYDAIPILDLNILESDKDSSKKTESLSDKIKKSLSILYK
                     TDIRNIEYMNNIKIGILIKFDDKKKNAFISREDFGLISDLILEMFCIDKKNLFKDDED
                     EWIENTGSEREKKLIAHFKEKERRKREKEAYHLCDYINIIQNIDGYVSINEILKMTYW
                     QLINTYKTKIQFKNYNESLGFAWSFKYQTDAEIEHWSKKIKILSSTVK"
     gene            complement(31166..31660)
                     /locus_tag="KRH39_000025"
     CDS             complement(31166..31660)
                     /locus_tag="KRH39_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509960.1"
                     /translation="MDFPNKMISNIGTTLMSNQDFAKFMIYNDESEKDILSMPDIKNP
                     VKELRNKKVFLNRRVEKVLKEADISVFIIMAEYRPCTTGSREIKKTKIEIGVVCHDEC
                     QNTANGLRDVALVCCIVDIITQNEEIAGIGKIKLENVYQMYNLSTDYNGFVITVSAES
                     FGDM"
     gene            complement(31675..32664)
                     /locus_tag="KRH39_000026"
     CDS             complement(31675..32664)
                     /locus_tag="KRH39_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509961.1"
                     /translation="MSYLSDIKKRIGLGCSTPKEKRILQLRLSFKKYLKETPTCIEIP
                     ITDIDEVCINEDTKKAVVAINDITNNDKRALDEKNLLVESDLNVDVGCYLFYDNCYWL
                     TIFKEHKEMDTYKHFIIKRCNQFFNYKYKGQMYKIPIAVENLTLYSDGMADNKYTSIS
                     DTKRQLYFGSNPVTKTIDIDTRIMLTGKTVFRVTSINDFEYNGRETGADGLIKAICLQ
                     DALISKDDTVNNIAWNDLSENDSVIIPFSKIIGDGFINLGEENEYRIDHSQGVKWLLD
                     KQYRYCDIIYQDEEKCVIQVNTLAKYSGLEALLLAKDKETNEIIDTKKITLRG"
     gene            complement(32666..33295)
                     /locus_tag="KRH39_000027"
     CDS             complement(32666..33295)
                     /locus_tag="KRH39_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509962.1"
                     /translation="MGTPLVKIYKKFLDAINDEEMLLLSNEIIEKMMYSYLEDAIVDF
                     NQCKKDLTIKYVDEKGEEIIPAAQLSYTSNYSNKNAEITLMGKDTKEEYELDKDYTIS
                     FEDEKFIISFVVETTEEIIFKYKYLGEIVSDLDLEEIKILSYGMQIHWLQPKINREEN
                     LKQMLTDSDYNAKSGANMLAKLQAREEQLRTRFNKYQQRYMLKNFEGWN"
     gene            complement(33310..33942)
                     /locus_tag="KRH39_000028"
     CDS             complement(33310..33942)
                     /locus_tag="KRH39_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509963.1"
                     /translation="MARAKKENISKTEVKANTLEKDIKSTLNKKEARLNRLELQRKLK
                     QKKQEIDIEIKNISTWDVDYIDPREKQQIFSLSKVGSKDSTEFIDLDTLYRIVRRSPG
                     FFEEHRLIISDVDSLDVECTPMDIIDFLGLNSLYEHIRNPNEDYLEYFLSDKVDINSF
                     EKILNKNNVELTRRLAERAIDLHKKKKFDSGLKAKLLAKRIGIEDLYLFS"
     gene            complement(33995..35005)
                     /locus_tag="KRH39_000029"
     CDS             complement(33995..35005)
                     /locus_tag="KRH39_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509964.1"
                     /translation="MATAKTLKRIAHELYTDTFREYTDRDGNTITYRDAESAIKNKLK
                     EMMGGEKYNYYKFQENKWGFYNLISELISDDINRLNEEVFSPFCDFENFDLGDKKEFT
                     VENTNLFKVANIADGVNSTRRQRLLNKKVPTTAFKLSVAIYEEAERFITGRINWVVFV
                     NRVSDSYHYDIARRIAKTFEGAYSTINAKFQATGNSDKVLLELIAKVEGATGKKPIIY
                     GTPLALSNLEGVQTDLEKEEKRKYGFIQTFRGGYKVLSLPNAYDENAAEGKEWALDNK
                     AIYVIPDGEKIIKLGTEGDVLVIENTDEKERDDQQIEYFMAQKIHLGVVTAAKFGVYK
                     IQ"
     gene            complement(35093..35590)
                     /locus_tag="KRH39_000030"
     CDS             complement(35093..35590)
                     /locus_tag="KRH39_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509965.1"
                     /translation="MKKDKAIIQTDKVKNPDVLTGKYIVPSEDTNPINLENGAVINIG
                     ELEDTEYGRDTHKIYKVTNDTLDWGIVDDPATMYDERLDERDYEVSPGQICRCRRLKK
                     GDEVTISLLHIADKSIAVKDKLQLKSDSFQFEKLPTADAKTPVAEVLELWNYEGQDSV
                     TIKVL"
     gene            complement(35605..37038)
                     /locus_tag="KRH39_000031"
     CDS             complement(35605..37038)
                     /locus_tag="KRH39_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509966.1"
                     /translation="MKKIKILKLPCNLKHYSDKKKLEELNSNLLPVYIYVMHEGTNPN
                     GTKFYEEAIDKAEPTLKNVPILGYVKINEDGKYDFDGHNVLTQVVQTDEGFILEEYYE
                     ERIIGVIPETNKYEKVEIDGQKYVKCKGYIYKSYSNHAYDIIMDSDEIEISMEIDIND
                     YQLDDSDGFYNIKDYVYHGITCLGSDVKGAMGSNCCLTKFSRKINYKEEISKICSEIY
                     ALEHGEGEKNLPNKNQKLNKNSEGYALAVSNLSTEIRNKLKEYKVETENWYGEKVEVQ
                     AFYYNDLIPEENIAIVEDEINWGYYYGIPYIVNEDAVILDYENKKSYIQTWREKQEGE
                     IVQVFSRQEKLKKEIIEKFTEKQKEISNLKASLEPLQAFKEEKEFELFKSKVDDVAQK
                     FELAEDEIKDIKIKAYNKEITLDEYKKELGYIFALKTLSNKQSEKENFSINDDKNNTI
                     KIPISNNNDSFSEPEEISFIKKYSDKE"
     gene            complement(37045..37236)
                     /locus_tag="KRH39_000032"
     CDS             complement(37045..37236)
                     /locus_tag="KRH39_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509967.1"
                     /translation="MSKFIYAFSEDDKKLLMEKGYRFICENKLNNKTLYVFENKSKLI
                     NNFSNEEMKRFIFTSKIRF"
     gene            complement(37239..38717)
                     /locus_tag="KRH39_000033"
     CDS             complement(37239..38717)
                     /locus_tag="KRH39_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509968.1"
                     /translation="MGKKNNNKSKSKDNNSTQNQINMLNAQIGKYASVKESTTLATEM
                     YKLRSISRDKLRKALSNPYQISNTDILQDASMILKATSGTYRRVLNLISNMNTFDHIL
                     YPKDISRLKTKEKITKAYMNSASQLEKYNIKFTSAWITEKVLELGEVYLYKIEDNSGM
                     VLQQIPAKFCMITMIENNVMRYAINIKKLTDKNIISFPEEIKNIYKKYKSNLLTREEL
                     IDNTYFQLSDKAVAFNYDLDSVKGVPFFCFIFDDLMELEDMKDLKSTNAVIESIKLIH
                     QKIPFGKNDEPLVDLNLIPIYHNSTKANLPKGTAITTNPLELETHTLSDGKSKINDYV
                     KEAKEFIFDNAGINTALLNSDKINNESILNGIIADSLIPMRIQQMIENWVNFELNKDK
                     KANLFNMKFIGTTHFNKMNLSKQYREDMGYGGSKSLFIASTGFTPLQAINTLQAEKLM
                     GFDEFLIPQQTSHTQSSGRPDKSDIGTDNGNSTQAKGNGEND"
     gene            complement(38734..40482)
                     /locus_tag="KRH39_000034"
     CDS             complement(38734..40482)
                     /locus_tag="KRH39_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509969.1"
                     /translation="MRTKYEKRELSSNDSSRNLLESIGEYWGAFYLANPHRFCMDYFG
                     FNLHLFQQILIYMMMKSDQFVFIASRGLGKSWLLGVFCCVIAVLKPGTCVLIAAKRKK
                     QAKLLITSKILGDLYLKSDTLKREIKSFQVNAQEVSIDFWNGSRIEAVVSNDDARGYR
                     ANVLIVDEYRMVDEGTVNDVLVPFLTNPRQPGYLQNPKYRYMQEENKEIYLSSGWYSQ
                     HWSYKKFMETVKGMLSGEDMFACSIPFTCSLEHGLLTKKRILKEMKKESMSDASFMME
                     YCGVFYNESDDAFFKSSWVNPCRVLESMFYPPSDIEYLENKKKRDKKYHLNKIKGEIR
                     IIGADIALARGVKNDNSIYTLMRMLPNEGTYKRCVVHIEAYNGMEAEKQAIRLKQLFS
                     DFQADYMILDTQGIGTTVWSYIQKANYDSDRDEWYDAYTCFNEDNTVDKSLAKKSLPV
                     VYSMKAYADENHKMAMSLRDVLTNRTLELPISDIEAKEMILEKEMIKADEIDKKAELE
                     AKYIAPYLQTTALVNELINLEYSADGGKIKIKEKSGARKDRYSSLAYTNFLADYLEEK
                     EKRKNRNNQKTVMIYW"
     gene            complement(40472..41371)
                     /locus_tag="KRH39_000035"
     CDS             complement(40472..41371)
                     /locus_tag="KRH39_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509970.1"
                     /translation="MAISKTKCLNCGEEKYTDRYFWKSHSEIFTLNKRLPVCKECFRA
                     RFLLLNGCYNGELVKALKHICFNFDVYFDEKLAKELSDKKNKDELIDEYMKIINRNSK
                     YKGKTSLDNLLKEIYTGNNNDKNIVINDEIKLKWGRGFDDYEYKILERKYKEYKEYYE
                     PESLTERKLFEEICIIELEKDKSREKGDMKAFNDLSKLVSSKMQDAEIKPSQKKKAGD
                     SQDDTFGMKMMIYEKNQPVKDRLKEYEDVDGFEAYVNKHMKKPLAVALGLATGKYSIN
                     DGDKDIKFKDDVRDILESNKNED"
     gene            complement(41502..42701)
                     /locus_tag="KRH39_000036"
     CDS             complement(41502..42701)
                     /locus_tag="KRH39_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509971.1"
                     /translation="MEDILKKKEDESLVDYRIRIRLAKVNKEIDLDWGEIVELLGLEC
                     SPDHCRKVSYGLKEAFDYLNSKIQNNSTQEEIDKINEKILELKKIKVQLSDERSLVNK
                     KIREYSRMDNIIDLFNNKIDDISLHKPFLSDSSYKCYESSNQEAIMLISDIHYGLETI
                     NAFNRYNSKIFKIRIQYLKDKIIEYSKLHKINRLHVMLLGDLISGHIHNSIRLENREN
                     IVEQIIEVSEILSEFIYELSKEIDKIIVYSIGGNHDRVLPKKDENLDKDNFTLLIDEY
                     IKLRIKSLDNVIFQENIYDNDIIVTRICGNTCFGVHGDKDKMSTAIPKLTSLIKLIPD
                     YIFMAHLHNCKEDNYGESEICVNGSFSGTDSYAKNLRLSSHAMQKLMIFNESGRICTY
                     NINLSKI"
     gene            complement(42778..42966)
                     /locus_tag="KRH39_000037"
     CDS             complement(42778..42966)
                     /locus_tag="KRH39_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509972.1"
                     /translation="MKNNKGTILEKCEVIKQEDGTYKFIEHTKNGDNEVLSSDVFDQY
                     IGKTDLNITIRLSETIVK"
     gene            complement(43339..43956)
                     /locus_tag="KRH39_000038"
     CDS             complement(43339..43956)
                     /locus_tag="KRH39_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509973.1"
                     /translation="MVKYNVHKALAEKKLLEDKIDRCISNFKIVGTKKGSDKNVYETK
                     TSVEDFNVEVSSKYQQIEDLIYNYNALDKAINISNAITNVKIGNKNYTVLEAIKRKNS
                     IELDKSLLRQMVSNYDCMMSEVNRRNEEVQKNTDRMFEEKEKSKDGAELISFYKKQQE
                     WSLVDPLKVREKIEKLRDEIEEFEKEVDFALSTSNALTIIDVDLK"
     gene            complement(44047..44325)
                     /locus_tag="KRH39_000039"
     CDS             complement(44047..44325)
                     /locus_tag="KRH39_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBG8509974.1"
                     /translation="MKKVDFLKEVAEELGVSQIRVREILDVIEKKRNEVLQDGEELTL
                     MGVKYLTKIQKGKEGEITLKDGTKKPWKTEEKRVPKLKIAKSLKDAII"
     gene            complement(44459..45901)
                     /locus_tag="KRH39_000040"
     CDS             complement(44459..45901)
                     /locus_tag="KRH39_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509975.1"
                     /translation="MIYNNNNFYEEIDGKSAQDYIKNLDYSIDNSKDRIKYIEERLGV
                     KHTWFEESNRNYIDTFYNTLTKRNENIITRVKYQEKKQFNDKFWENIFEQTSDSPLDK
                     DGVYYVTIDGEEVVMDYNRFITWCGYNQINPIEYINVKNPFSNSNGTWEYTHHNTSKI
                     KLILNKDDGIYSTSNIAKTLEIMGSYILSVDDKPKETKIRIYNSKELFNRACQEEALL
                     NKIAPANGGNINSNTSNKDGFLEDNSFAFFQLPKNYKKIKDIKVKPKDIKKYPIIKTY
                     SDTYEWYKAKYKELGHKQLSKEELKLKRMVRKNLNALKNDMTDVKNSIERPIIWKAPL
                     ADAGSPEWDYLDMFDKSHVKELLKIQKGNDLQDDLTCIVMDLNNIISKIEFTTVQKDI
                     LNLYRKDKSLEFISNLMNITPQAINNQINKIVNKIIDAYEKNYEENYYYISVCKGKYK
                     KCSRCGKIKLLSRFDKNGKKGYRSNCKNCN"
     gene            complement(45904..46152)
                     /locus_tag="KRH39_000041"
     CDS             complement(45904..46152)
                     /locus_tag="KRH39_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509976.1"
                     /translation="MKEKKIELIYLEDLDESEKTGVHKNRYQILNEEYEQMIDTYTEM
                     SKRSSYDIVNERHKEDFKDELWDYDDTDYDDGLQGKEV"
     gene            complement(46330..47193)
                     /locus_tag="KRH39_000042"
     CDS             complement(46330..47193)
                     /locus_tag="KRH39_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509977.1"
                     /translation="MKRGEVLEIGKRKKTFSPEQRAIINNKKELPYHNFKLGGFVFCT
                     YVKNELLFNEVNISKANISRLIYLATYLNYNTGEENLLVRYSQFKEMIPIDRKNMREI
                     LNLKDRAFRNFLADVKKCELLYESNNKFYINPKYFTKGIPNFENKEYTRIYINTSRFL
                     FEHCTPRQHKQLSYIYQLIPYIHFESNILCLNPHETDISKIKKISLYKICQLLNISTI
                     KQDMNKFKKELLRFYIKVEDDKYYFFKYVIVEGNNKNTDYFIINPCVIWKGNNLNLAK
                     KTIDLCFFNCI"
     gene            complement(47465..47737)
                     /locus_tag="KRH39_000043"
     CDS             complement(47465..47737)
                     /locus_tag="KRH39_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509978.1"
                     /translation="MKKSSSFYLEEDIFGEIEKYQNDKNISSRNTALERIILEWKNLQ
                     EENKLLKQCLGNGVYRTNKKTSVTEEKKDIKKENPIIKNIFNNMPD"
     gene            complement(47750..48664)
                     /locus_tag="KRH39_000044"
     CDS             complement(47750..48664)
                     /locus_tag="KRH39_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBG8509979.1"
                     /translation="MEENYQVIGLDIGRGYVKGYSKYNGMVKECLFKSVFGDGRNIDF
                     EKYENPIYIDFEGVSYFIGLLAEKESITPIRNSDDSKVSLTMRILVAAALNEITVADE
                     VKIMLGVPYKSFRKATLKEVLEAYKGKTFKIKDKIKGGYKEIKISDISIFREGDAALY
                     HLLEGEVNEDKAVGVAHIGFRTTELSYFEKGFVFNDKLSDTLEAGNQDALTIVQKQLK
                     DRGIIRELNEIDSSSDYDELKKAAYIMASESTAQRIASKWKNIDEMDVYVSGGTALHM
                     TFDNRFKVSKDAQMVTAKGLFEVGMEQF"
     gene            complement(49086..49274)
                     /locus_tag="KRH39_000045"
     CDS             complement(49086..49274)
                     /locus_tag="KRH39_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509980.1"
                     /translation="MESGLFVSRILCVVLSFPHFKKIADGFTDEIALYFPHKTNIYLK
                     IFLRFLLLNYILNKENIK"
     gene            49771..49929
                     /locus_tag="KRH39_000046"
     CDS             49771..49929
                     /locus_tag="KRH39_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509981.1"
                     /translation="MRITKGKIKELIELCHQLEKLFVQVFKSLEHVLISLCSCIGWLL
                     ILIYIIKH"
     gene            50182..50373
                     /locus_tag="KRH39_000047"
     CDS             50182..50373
                     /locus_tag="KRH39_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8509982.1"
                     /translation="MKLEKLIDIRKEKKYSQEDIARMLDISLRNYHRKEKGENQFTVS
                     ELEKICSYLDIESKELLDI"
     gene            complement(50587..50898)
                     /locus_tag="KRH39_000048"
     CDS             complement(50587..50898)
                     /locus_tag="KRH39_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8509983.1"
                     /translation="MNRLRELRKEKGYSTQQVGELLGVHYVTIQNYETNRRKIDNETL
                     IKLSNIYNVSIDYILCLTNNRENIQLEEEEKQLLNNYRELDTKSKTIVQEQVNTLKKL
                     L"
     gene            51484..51786
                     /locus_tag="KRH39_000049"
     CDS             51484..51786
                     /locus_tag="KRH39_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509984.1"
                     /translation="MDEKILELLQKMDTSINDIQGEIKWINTRLDVMESDIKDLKTGQ
                     VEIKDDLKEIKGDILKLDLKTEDTRRNIKLMQLDINKIETITNYNLNDIATLKAIK"
     gene            51800..52102
                     /locus_tag="KRH39_000050"
     CDS             51800..52102
                     /locus_tag="KRH39_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509985.1"
                     /translation="MDEKILELLQKMDTRFDRIENRLDSMDGRFDGIENQLSDLKQGQ
                     EEIKKKLDLTYNQVARNMEGITEVGEKINTLKNDMNFVEMATSKNWNEIAKLKSIK"
     gene            52114..52347
                     /locus_tag="KRH39_000051"
     CDS             52114..52347
                     /locus_tag="KRH39_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509986.1"
                     /translation="MDEKTLELLREIQESIKILNDKLDEFDYTQDKIKSNVEGLLECF
                     SRIDFRIEDLENGQTSLYTKLDIVQNETAKSIK"
     gene            52441..52629
                     /locus_tag="KRH39_000052"
     CDS             52441..52629
                     /locus_tag="KRH39_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509987.1"
                     /translation="MYEQRKAKVIFNKSGGNSSENSYTNRVTIPTMWIKYLDITKSDR
                     EVLLTFDGNKIIIEKIEE"
     gene            52906..53217
                     /locus_tag="KRH39_000053"
     CDS             52906..53217
                     /locus_tag="KRH39_000053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509988.1"
                     /translation="MDEKILELLQKMNNNITEMKQDINDLKIGQVETNNRLDNMDKRF
                     DSIENRLDSMEKKQDILYNQAIATAENITEIREDLGDVQVTTTCNLNDLKELKKLKSV
                     K"
     gene            53315..53509
                     /locus_tag="KRH39_000054"
     CDS             53315..53509
                     /locus_tag="KRH39_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8509989.1"
                     /translation="MNLQRLKELRLEHGYTQQQLATHMDMSQTTYSDKEKGKTPFTIP
                     EFEKLHKFLGEEVLELLELQ"
     gene            53665..53922
                     /locus_tag="KRH39_000055"
     CDS             53665..53922
                     /locus_tag="KRH39_000055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509990.1"
                     /translation="MKNLYHYFNLEAFARDLEPYKSRIIDDYIDGDEIEYYNNMDTIE
                     FAKLFIGESENIKYLAKEVLERYFDYDKYRKDLNSTNTIIK"
     gene            53994..54131
                     /locus_tag="KRH39_000056"
     CDS             53994..54131
                     /locus_tag="KRH39_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509991.1"
                     /translation="MKKLIIKIKSLFKKDLDSSYWSTTESIISKYGNSNQLELLEQAN
                     K"
     gene            54144..54380
                     /locus_tag="KRH39_000057"
     CDS             54144..54380
                     /locus_tag="KRH39_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509992.1"
                     /translation="MINYSEYMTATDLINICDLLEGEIIGEAGTYNSLYVRLGNLIGL
                     DWALKGEELRQQLIQNINNMMYLDNENRIQYKEF"
     gene            54442..55053
                     /locus_tag="KRH39_000058"
     CDS             54442..55053
                     /locus_tag="KRH39_000058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509993.1"
                     /translation="MKKILLFEGAGMNIYEEGSDVENYRIRTAFINGNGDKIYLELGG
                     NLQNCKSVKRFGWYTHIDHCFNIGVSTDENVSRIKLNNTFFKGYSKRDLTNYINENLN
                     CNFDTIEVLDWMEGYRVHGNRKEYNLMDDHKLNRKRTIARNKAYEKIDKEIMEKLGEK
                     YSKISLYSMDEDSITVRCHTYEKDLYKIGMSEDNRLLKIQVNY"
     gene            55128..55391
                     /locus_tag="KRH39_000059"
     CDS             55128..55391
                     /locus_tag="KRH39_000059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509994.1"
                     /translation="MLINKAKGIKTSLETSSLRELKGYIKDLRKALSGYKYKNKIDEI
                     TEYQIDKLCYKIDNTDIFDLKYKEIEKYIDNLILIINFIFMEM"
     gene            55429..56013
                     /locus_tag="KRH39_000060"
     CDS             55429..56013
                     /locus_tag="KRH39_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509995.1"
                     /translation="MNEYILISYTKGEGTCDHCGRTIRNIATIKNNVTGEVFHVGLTC
                     VEKIMKLNVTFYKALSREIKKHYKCIEYYSKGLDIETNLNKIVKNNTKYKEGSYAYKS
                     NEQMLEDAIAEVAWSLARMIDSCMRMNKLSKSGLIDIDILNSLFIKYKEYMSKFDENY
                     NKNKYKYKSCYTMKPLKVILDENEDLKELYKSMQ"
     gene            56331..56855
                     /locus_tag="KRH39_000061"
     CDS             56331..56855
                     /locus_tag="KRH39_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509996.1"
                     /translation="MKNLKSMVIGMLISLIIVGVAGITEVKAAELDNFYVESTDRVVE
                     ILEDESVVLYDTKEQVYNFYPKCLGDWNYSFYNKKDLDRAVATYKELSNNVSHSKDVY
                     VINKLNNNENIKVFLSDGSSIIYIKNDNKYYFYPACMGDWYLTLDSEINLSDCVNTYV
                     EHSYILLDGGVVSE"
     gene            56848..57078
                     /locus_tag="KRH39_000062"
     CDS             56848..57078
                     /locus_tag="KRH39_000062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8509997.1"
                     /translation="MNNNDKLSILNQLEYIEKDINARNEMIEKFKDLLVKYPKSRDIR
                     KYIKHQTQFLNGIKYVYDNLDILKNMINNDKK"
     gene            57249..58058
                     /locus_tag="KRH39_000063"
     CDS             57249..58058
                     /locus_tag="KRH39_000063"
                     /inference="COORDINATES: protein motif:HMM:NF022027.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HBG8509998.1"
                     /translation="MNELKIKGKQKFMNLEIPVIEGGFGEDKKCMTDKTISEIHNQPN
                     FKIRERIKDNIKRFKEDIDYIDLKVIRDTDDNLELLQTLGYSKMQISKADNIFLLSER
                     GYSKLIKIMDTDLAWEIHNKIMDEYFSMRKKLNDPFENLSPELKVIIKLEQRQAELES
                     GQHELEEKVNVLYDFSTIDYNQQETIRSLVNRRVIQVYQCLNFSAYEDAKLRKRVYFS
                     LWKDYRKKFGVNSYKNTAKKNYDNAIKFIENWEPNYDLSNEIYMFQKVENI"
     gene            58336..59052
                     /locus_tag="KRH39_000064"
     CDS             58336..59052
                     /locus_tag="KRH39_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG8509999.1"
                     /translation="MNILIENKFIEKLKYTKNIPNSLYKDLRHNFLFHSNKIEGSTFT
                     SESLQDLIEHNIVKGDHTLDDVITTVNSSYVFETVIDTLGEYLTEKYIKDLHGQLMYN
                     SNIHNMGLSGIYKKIPNRIIGTNSVLAQPFEVEPKMLELIEWYNNLKDITLKDLAEFH
                     YRFEIIHPFQDGNGRIGRFILLKQILENNLKLIIISSDTVDDYRQALKGCHNEYYKPL
                     EQYFESLDDYKEKNKDLWSF"
     gene            complement(59294..59593)
                     /locus_tag="KRH39_000065"
     CDS             complement(59294..59593)
                     /locus_tag="KRH39_000065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510000.1"
                     /translation="MVIKVNFGGNYSPDYIYVPNEISLKKYEIRNEFHIWIDNTEDEH
                     PFWCYENGVQIGLHYRGDAIVYWLNNYILKNANDKARLIESFSNKKLDYDLIINL"
     gene            complement(59789..59971)
                     /locus_tag="KRH39_000066"
     CDS             complement(59789..59971)
                     /locus_tag="KRH39_000066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510001.1"
                     /translation="MLKTLFILLAVFVIIKLSILIFKDALKFLNMFESKNESFIGWLF
                     LCILALVIYNLIDIIL"
     gene            60167..60739
                     /locus_tag="KRH39_000067"
     CDS             60167..60739
                     /locus_tag="KRH39_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBG8510002.1"
                     /translation="MTWNDLKNNDITYIVEYINNKLNNSKSLTKVAIGLGVSESSIRK
                     YLTKRGYKRINDEYVFIGDNSMTKVVNNRQQSNNDNTFNMTIEDDNNHNIVIDNQFKN
                     NIVSLAKDYDKIQDVLNWFENKEDTNVIEVVQDGIKIDLHSKDAIRTTVRLNKDAWNL
                     FDEFCGKFREFNKSDLMSMALLEYIKKYDK"
     gene            60872..61798
                     /locus_tag="KRH39_000068"
     CDS             60872..61798
                     /locus_tag="KRH39_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510003.1"
                     /translation="MNYNLKNENVLSELLQNEDEKTRIGILKVYKVLIFWFAKEGKLV
                     EEENDYIVEIVGNDKEEIVRDMSQNKTYNAIMAIIFKINKIFKTLNYDIHFSKPKNLV
                     AEFDLSKYLRQDEVLQVCSKLTNAQDKFIIYAAFCGLIGKDAEDLRGIKIKDIDLEKR
                     LIKLKDTEFKLDKFLFDFTKDTLEQKEYKQYLEQDSNAAEFYLFNSNSEYLIKTKPDA
                     RYGNGLKEMGYSTIRKRLLIINDYLDNKVQLNFTNLKISGIMHQMHLNEKKYTQSGYW
                     TNKSLKEFKEDNNLNFKIQEVLSIYKQKYIKE"
     gene            61803..63371
                     /locus_tag="KRH39_000069"
     CDS             61803..63371
                     /locus_tag="KRH39_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510004.1"
                     /translation="MSKDVKENISNEQKDLNKINLIYHIIYDNDRFKMITRKYFEIFR
                     LKGMSAEEISLKFEEFLYSLISDKFKKINTVNDVKKLRNQENSSSLKLKKRWSDMTLD
                     SLSEDTKSSRKKSSKYLKKELEIVESEYLKNGRIISAGVKNPKELKEQRKDFLDEWKS
                     SSSYNICDYPYLQELSTSKINSAFIHDILMCITEVLKDDYDFDINKIAIKTPPHISPG
                     IFLPFTKGKRYSHLNVKKNENEYMSDDYSAKNLSGKQMNFFYKFSIGQDENIENLKLE
                     LINPDTEKRALPKIYLDKQDLEIIRFVYTYSHMNSFSFYLGDLVKYLGLSDGKKNYIN
                     IKNRLLKLPYYTFYSNQTNDKGELKYEISFNLFSSTTIVNDENNNNRELVKITKSFIE
                     EVERVNTDIMYRNELEKLQLTQSINLAYFLEGRRSFLISCGEDIKNKSFRYDIEDLKF
                     DVHLNKSKTVKQNMEYLELGFNEIKENQFIIKDYKRGNSYFDVYFYEDFEKRKRLINN
                     TILSLPDYMIENKS"
     gene            63845..64111
                     /locus_tag="KRH39_000070"
     CDS             63845..64111
                     /locus_tag="KRH39_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HBG8510005.1"
                     /translation="MSLKYKDKEFVLVEKKNINELDNSNIKYIDLKDKQYYVVTQGKK
                     PKRFNLEDIKNIKKDLDNGISLRKCATKWNCSTRTIQDIKNNIY"
     gene            64187..65365
                     /locus_tag="KRH39_000071"
     CDS             64187..65365
                     /locus_tag="KRH39_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510006.1"
                     /translation="MQELKPVARISKSKLKDILDVFKTSIRKVEKIDYSACNSCAIKN
                     NPSFDSHKLNEFCSNCPKLKTISEFSYVTEKETELGTIEIVTNESKCMALSKSAIKQY
                     LAYHFIVSNKNYVRKAVSFQNIADICNVSIVTARHNHNVLLRVGLVYSTEIKRGKFDI
                     VIDEEYKNHLKKEDGGKGYLTMSLDTLNHLLSFENVNELKLEIKKLIWADAKGSKLGK
                     RIRFKKDNLISILPEYIKKSNKRTEAFLRSSKTLFDVSNDTIDTKRYETKETISEKYT
                     EPIRIQISKFFDNTGCSLSSNYSKVLSQINLDRYNLFEEDLRSKLDMEKDIIINDLVG
                     LSVQYGVTRVLAALDYMFNKYTVDEEDINYNEIRNPGAFIRTLIVQSINKNGSLAIAI
                     "
     gene            65973..>66737
                     /locus_tag="KRH39_000072"
     CDS             65973..>66737
                     /locus_tag="KRH39_000072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510007.1"
                     /translation="MDRFEVLKYLKFNVWHSEWPLELLKGAITKDNATGNLLLQLKMC
                     NISDKEIKSAYFTIECFNDAGDLIDNNLKYVYQDINIKPHECLGDDIAIKINDNKTRA
                     INIILEQIVFSDDKVHKYEREEGLIIPKLDKIELLNIELLEELKKDYEEYDFQYEIKY
                     MPNIIEKKAWSCCCGKINKLEKDKCDNCKRSKILQIKKINKNYLESIYKKSLKKKKEI
                     EIKLEKDNRHKLKNINKNNRRTSILLISILLIFIIFI"
BASE COUNT        22338 a        10241 c         7555 g        26603 t
ORIGIN      
        1 aaacattata accacacgcc cagatacttc tgtctatctt tataccgaaa gtataaccac
       61 tacctggact tattactata tctacaaaat cataattatc aaaatccttg ttaaattcgt
      121 caaaatatat atctgtatct aaatatcttc cacgttgtaa gattttattt tctttcatta
      181 aatcattcct ttcctattta acttcataag aaaacttttc aagttctata ctattagctc
      241 cagcatttgt acttgtgttt gaaatggtct ctataaagtt atcatctaat tcatgtatgt
      301 atttcacttt ataacaagaa aaaggaatgt ctattttatt aaaaataaca ttcccttcgc
      361 taccaaatat tcttcttcta tccgtatcta atcttaaatt atcaattgat aaaatgtttt
      421 ttgaatctgc taaattgtca taccattgac cgacttcatc cactgcacca gattctaatt
      481 ctaaataaat taatatatct aagactttat cagatagagc gtcatacttg gcttttaaac
      541 tgtctaattt ttgacctaaa tttccaccag cttcaagaat ttttacacta gcatcaagtt
      601 catttactgc atctgatata tttttagata gtgtacttaa ttcactcgaa ccaattttat
      661 tttgtattaa atttatcttc tctaagttag tagctatatt acttttattt tcatttattt
      721 taattagact ctcatcaaat tttgcatcaa ttttaacact agagtaagta ctatctagtg
      781 ttgttatatt atcatttatc gtatttggta taacttcttt atttgtatta ccatttacaa
      841 cagtcactct tacatttata ctgctttcat tgaatatttc tatcgtatta ttatctataa
      901 tagtataaga attagtcata cttttcttag ttgcttcatc taatattgct acaaatatat
      961 tttctgtaac taaattatga ttaactataa gtttaaaata ttctccatta gtaacccagt
     1021 ccgattcaac ataagattta ctaaaagcta cattagttgc agcagaagtc atttctgcta
     1081 tcttctcatt aactttctca aagtcatatg tcaatctatc ctttaaagta tcctttacag
     1141 ttccatcaat agtttcccta gattgcatta actcgcctct agcatcaagg ctttctaatt
     1201 cttcaaacct attttcaaac tcagttattt tattactaac agttgttgtc atatcagttt
     1261 tagcagtatt tacttcaact attttgtcat ctacttcttt tatctttgta tctaattttg
     1321 tagtactttc atcaatacaa tcttctacat tagatatagc ctgatttact tcatcaagtt
     1381 tatagtcgat ttgttcttga ttatccgtaa gtttatcctc aaatgtttta tcatataaat
     1441 caaaagtatt attagcccta gtatctaaat cttgaaactt cttatctgtt tgagcttgaa
     1501 catcaagcat tttgtcatca acaatatggt ctatttcttc agatttggca tctatcattt
     1561 catctaattg taattggcaa tcagtaagtt tatcatcaac ttggatttgc atattctgaa
     1621 tagattcttc acgatttgct tcttggtttt gacgtgttaa ctcattaaac tctctttctt
     1681 cttcttgtat gtttcttata tcttcattat aaatcctaat ctgctctgct ttatccctaa
     1741 cttcttcatt tcctaagata gttatttcaa gttgttcaac tcttttagtt aactctttat
     1801 tattttttat aatttcttct gcttgagata ttaagactaa taaaactcca tactcatctg
     1861 tactggtcaa atcatcatca ttaatcatac cctcttcaac tctataataa agatttgtag
     1921 tattaagcag agttccctct tttacaagag ataattgaaa cgtatttata ccaacacttg
     1981 caagtgcttg tcgactaagt tctatctcta caataccatt ttctgcatct acaacttcac
     2041 attcaccatt aactttttgt ccatcacttt ttgtaatgct aactaaaact ctatcatatt
     2101 cagataaatc tattatttca tctttatttt tcaatattgc ttcaatataa gctgttttaa
     2161 catcattttc attataaata aaataatcta gcaatctttt gtttattctg taattactaa
     2221 agtcgacctc tatggtatga ttctttattt cataattttt caaagtatca catcctattg
     2281 ttctttattt tgtttagttt ctactttttc tttttttaac ttatttatct cttcttgtaa
     2341 ttgctcatta aatgctttat aaagtattaa ttcattttgt aatcctctta tttcttctaa
     2401 agctattgta tatgctcttt gcatgtttac ttccataatt attatcctcc tttatatatc
     2461 caaagaatct ttaaattttt ctatgttttc ttttaaatag ttatatgctg attgaagtgg
     2521 gtttttaccc gttgcatcta gtgtattaat tccaaaataa ttatcaaaat tatcttctaa
     2581 aacagatata ttatcatatt caacagcgtc caatccctct cttcttgcat cttctgaaat
     2641 atatcccgat actttaatct gagccatctt attactataa gatatattta cagttgatat
     2701 cctccaataa taaacttgtt gacctgttgc taataactta ttctttccaa tcgccatgtt
     2761 gttcttcacc acccattatt ttgtaatatg tatgttcata tcctattctt ttccctatta
     2821 ctctaaaatc acatttacct gtatatccaa ctactttaaa ataatcctca cctttttcaa
     2881 caactctgca attagaatta gaactaaata ctgcaaagtt taatatactc tgagcaaaga
     2941 tattatttaa ttttattatt gtaccaattt cttcaacttc tatatcaaat aaaatgtctt
     3001 caattagaaa tttaggactg tcaattggag acataccaaa tgttgtacca tttagttcta
     3061 tacttccagc agatttagaa ccatttctag caaattgctg tttttcttct ccattaatca
     3121 taaatgaaat agctcctctt ttccagatta ctatcccatt atcatcatcc caatcttcag
     3181 aggtgtaaag gcgaaaattt cctcctgtta aatccattct tgtatatccg tcatctccaa
     3241 catctatttt atttgctcca aatttagcat tatttgaacc aaatatataa ttttcatatg
     3301 catttacata tatagcagct ttaccacttt caacgaaaaa ataattgcta ctattgtatt
     3361 taaaacgaaa tgctgaacgt tttccatatc catccgaagg tcttgcatct atggcaaagg
     3421 aatttttact gtcactaaag aaacttataa ttgcattatc atcgtcaaca gaagttatgg
     3481 cattacacgc tagacttcct cgattgacta ttaatccact tctatttatt acaacataat
     3541 cagatatatc attaaaacca aactgaattt tgtctgattg gattccaaca attgccttat
     3601 ctgaattcct agtaactatc atagagatat cgtcttttgt aagtttaatt tcagcagcat
     3661 ttgttttagt tgccccatca actctagcca cttctaattt tatctcttta gctgtagcat
     3721 ttatctgtga aaattcttct gatatacttc cacccatccc tgttatgtaa attttatcca
     3781 ctgtattttt aagttcattt atctttaagt tatattgagc atttgcattt atagctaaaa
     3841 ttctgtctga aggtgtaatt atactatctg aaataactga attgattata gagtttagat
     3901 tagtacttgc tgcatccaat gctgtttttg aagtgtttat tgcaactaag tctactccag
     3961 tagttttatc aataagtttt tgtaactctg catataaact cgctttatct gtatctaata
     4021 aattaagttc aatatctatt ttatcttttt cttcatctgt aatctcatta tcattaaaat
     4081 aattagagag atttgaacta aatgttccta aatctgtatt caacttaatt atagctttat
     4141 ttatattttc tcttaaggtt atattttctt gtgaaccacc aaaattttta gttacttctt
     4201 ttactaatat atttacgttt tcagctctct ggtctataag agacatattt tcttttatat
     4261 gagatgcatc ttcttgtact tctaaaactg tttgatgtat tccatctatg tctttttcaa
     4321 taactacaaa tttttcattg tgtttttcat ctgtttcata caatgcccag atttgttctt
     4381 cagtattttt tcttgaatta ccatcaaaag atatattgaa tattgatgag tttataggaa
     4441 cttctgtatg ctttccattg tcatttacaa ctactaaaaa ttgtgtgttt ccctctggta
     4501 atcgtacatt agaaactttt aatgttcctg gattatcagc atcccagtat atatattttt
     4561 tatttgtctc tttatctact acttgataaa gtgtgttatt gtaagtaaca attactccag
     4621 taattctaat ccatgaatcg gctggtgaat tatctaatat caatttacat caactccttt
     4681 tgtatatttt tcttaggaat atattcatct ggtaggttaa ttcttttctt ctgttgctga
     4741 ataaataaat gtcttttaga gttcaatgtt cttgttgcat gttcagcttt agttaaataa
     4801 tcagctattg tccttttgtc atcttctttt atttttttat tacttaaagt taattctaag
     4861 gtatctaact tcccattttt taaattctgg gtaaaggatg taaaatagac taattcttct
     4921 tctccagatt ctttgctatg taacactata atatctccca gacttaaatc tcccttccaa
     4981 tgttgtctaa aattaatatc tataattcta tctaagaagt ttataacatc cagagtccat
     5041 tctcgtgttg gaatacattt taaacttaat tgccttttac cttctgctat taaatcttca
     5101 acttttaaaa aagcatcatt tgtataagta tcatagtaga ggaactcatt taattcatct
     5161 aatagagctt cattaaatat taaatatcca tcctcatcag ttgaagtctc cctcttacaa
     5221 agaatattta tatcttttat actttcattt aacttagcta tttcttcttc taaatgtttt
     5281 atttgaacat ctaatataac ctttttatct tctaattcac ttatctctac tgctaactta
     5341 gctgaattta cttcatcttt atgttctgga ttatcataag ttttctttat atccttttta
     5401 gcctttatag tttctattac atgtaaccat tcattgcttt tgctatcacg ttctctctgt
     5461 ttcttaagct ttgtatctat taattctctc caaattggtt ctcttatttc attcatttct
     5521 tggtatttct ttatagctct actaagttct tcactcattt ctttattgtc taagaaataa
     5581 gagtaattct caatgtaatc atatcctgta actgtagctc ctattatatc catatcttca
     5641 ctaccttcaa gcttcagtct agttactata tcgtcactat ttgtagttct ttcaagtgat
     5701 tttatgtagt tatcatgaga taaatataac tgaatgttat ctccaaaact atctatatga
     5761 tacaaattaa ctaaattatt ataggtgtca aaatcagcta tacactcaaa ttgttctttt
     5821 agttcattat tgaaataatc taaccagttg gagttaatac tttcttgcca tcttactttt
     5881 tctcttttat ttccttcact atcagtttca taagcaattg aatcatctac atggccaagt
     5941 ttccaacctg tctcttgttt taaataatca ttaagagata taatagatgt ttcttcatct
     6001 ttagtaaaca tttgtaatcc ataatcctca atatttacat caatctttcc tagtttaact
     6061 tctttagatt tagctgttac tacttttaat ttatcatctg tagtcacaac attcttaact
     6121 acaaaatatt ctttatcatt aagacaaata agacgttctt cttttacttc ttcaaaaacg
     6181 ggatttatta ttttattaaa taaaaatcta tctgtaatat attttggaat ggtaagttgt
     6241 atttcatcta ctccaccaag ctcacgcttt atcgcgttta aaaagttgat tggaatttga
     6301 cctagaaatg atttattcat cttgtgaatg gttaatgtgt aatcagattt tatttgttta
     6361 agattcacat taaaatgcat attttatcac cttctttgta ttagaaaagc ccatcaatta
     6421 agacgagcta aaatatttta ttaattgtaa aattatggta taatctagta tagtaaggaa
     6481 ttttgaatcg aaagtgctaa gagtggttgt ttctatattt agcctacttc ttcaaaggaa
     6541 ggaggtgagc ctcgctttat ttttttatag ggaggtgaat ttatggaaac agtagtgttt
     6601 aatatttgcc taaatattgc aacaagttta atagcttgtt acatatatga taaagcaaaa
     6661 aaccactcaa acgccaataa gagtggtttc aaaagataaa ttgatttttt aattaacttt
     6721 aaggaacaat cactctttgt attaagtaga ttaaattcct tacttttatt atatcataaa
     6781 tacgtttttt gtaaaacaga ttcgtttaca gttaataaat aaaaaatcac tattactgat
     6841 atcaattaat acatgtttta aataggtata ttataaatca tttcattatt caaaattcac
     6901 ttcaatatct tcaaggtctt tttcattaat atttaggtct ttcagaattt cgctttgaga
     6961 tattagttct ttttcatcta tacctttcat tcttatttct gtctcaataa gaagcttaca
     7021 atcttctata ttatttatca tttgtaacac ctccttaaat atataccaga gtataaaaat
     7081 agccacttgt ttaaggtggc ctagataagt agaaattctt tatgtaaaaa tattttaatt
     7141 ttctgttacc tgtattatat ctttattcta ctgctgtcgt caatataata ttaaatttaa
     7201 catattggta atttatggta taatattttt agggactaca taaaaaacta ttagggtgaa
     7261 tcttcatata cataagcctt cccatgtcaa atcaagaggg gaggtgatat tatgaaatat
     7321 ctaatttgtt tgttaacgat aataacattt cttacaacta taattattag tttgattaag
     7381 ttggttcaga atttgaatag actccttaaa gaaatagatg agtttaagaa aacgttgcaa
     7441 aaaattaaga atcacccgaa cggcaattca ggtgattctt cctctaatta acacttcgct
     7501 tatttatgaa gagacacact agcacaaata gttgtagtct ctttttttta tgtctttatt
     7561 atattactat tataccctaa tatggtcaca atagtcaata ttgtcatagt agacaataca
     7621 gagaaataaa aatcactcta attgtagaat gttttctcat tgatatttat actatatcca
     7681 ctattaactt tgaatctttt taaataaatc agaactatag tcatttatat cagagatatt
     7741 tattatacta tacttagatt ttatattatt aaagtattca ttaataacct ctttttctat
     7801 aacattacta gaagattcat atttgtctaa accttgtcta ttaataaccc aaggtttttc
     7861 ctcgtgtgtc attctttcta atatttttcc actataacat ccaaagtatt taatcacagc
     7921 atcaacaatc ttttcttcca tatcattatc taacgaaatt atattgtttt catactctat
     7981 ttgatttctt ccaaaatctc tatatttctc atatacattt ctgtatacag ggccatgaac
     8041 ccatgcttca caatcatcat caaataaatt gttattagta aataatctag taaatgcttg
     8101 tgaatagtat aatagttttt gtaaagctag aggtgttatc tcttcacatt ttgatattat
     8161 atattcggca acaacaccta tcttatcatt tttaaaaaaa gtgttttttt cttctatttc
     8221 tagaatagct tctctacatt ttttatatgc aatatctttt atattatttt tattttcctc
     8281 caatatcttt ttcatttcat ttgtatcatc aagtattaat ctcaatttat cggaatatgg
     8341 ttttgttgga acatctcctt ttaaatatct tgttatagtc ccttctcccc atcctaataa
     8401 tgaggaaaga ggttttttac ctatgttata tttttctaat aaaagttcaa tttcgtctac
     8461 agttataatg ttatccattt ctctaatttt agtatataat gattttaagt tttcatcatt
     8521 aagctcatga atatatacat agtttccaca ttcttcacaa aacgcttctt tcttcatgta
     8581 ttttatttct ttgcctctaa cagtatcaat ttcttcttta attttaattg tatagtttac
     8641 atcatctaga caaaattcac aaaatacttt attattatca gttctcatat ttttcatggt
     8701 ttcccctcca agttttcctt cttatttaat ttgcataaaa aagatatatt ttttatgtat
     8761 ctcatatact aaaataatgt gaattttttt atttaatagg ctacatatag tatttaattg
     8821 aatactttgc aggatgaaaa gatattatta tcgtgaaatc ttcttttgtt cttttctttt
     8881 ctgcaatgta aaattttatg tataactcca cttcttctct taatccatct atatttgtaa
     8941 attcatattt ttttccaaaa aaatacaata ttctaccttc gtgatttcta tgttcttctg
     9001 attttgaaaa atcttctttt gttaaacaat ttaacatttc tatttgtttg tttttatcta
     9061 gattatattt ctttaaaaaa tcattattat cctgtctatt ttcactttga gatataataa
     9121 aattttcact ttctatacat gccttaaact tttttagaat tatttctatg tcttctagta
     9181 tcgctgatac agccaagcag acacactccc ttcaaagaaa tatgaaaata caattttgta
     9241 tcttctatat attatagaat aacacttttt ttagaataat tcaatataaa gcaacaaaaa
     9301 tagtattaat taatactaaa aatattttta ttttaatctt tttccagaaa tgatactttt
     9361 ttgtagattc aattaatcaa aatttatttc tattaatttt actcttttta ttatgattta
     9421 atattttcta ctatttttct atatactcta ttttctaaat ctctaccaaa cttctctaaa
     9481 tctttcatag tatccttagt tacatttcct tgaacaacaa agaatggtga attaaagttg
     9541 actgatgatg ttggagacac acttcttgaa cgagaatttg aagaaggatt ataattaggc
     9601 atagcaaatt tacttaaatc taattcatct aatatgtcgt taatatcttc taatgtatct
     9661 ttaccttctt ttagcttatc gagccattcc tgcttagtga cagaacccat cgccaacata
     9721 ccatcttcat atttatttat gtattcaata atagcatctt gaagtttctt aacctctcca
     9781 tcaagaccta caaatttacc acttgaaatt gcttcattaa ttaaatcaac taaattttca
     9841 tctgagtatt ttttctctaa ctcttctttt aatttatctg cttcttcttg tagtctgtca
     9901 gactccttat catacatatc attgacttgg tcgtccacat ggtcttgtac taaatcctgt
     9961 aatttcttct gttcttcttt taattgtttt tgtaaatctt taagtttctt ctgaccactt
    10021 aaagaactat ctctttcagc tattgctatt tgcttttcta aatcacttat tacatcttgt
    10081 tgttcttcat aatcatcttg atatttagct tcttttcttg agtcattgta agcatcttgt
    10141 tgctttttaa gagaatcaat tttagcttta gtttccttgt ctatttcttt aagtctctct
    10201 tcaagttctt ttttatacat gtctctaatt ttctcttgta gactttgagc ttctttgagt
    10261 tggtctttat aagcatcttg aatttcttta tttaatgctt caatttcttt cttagattca
    10321 gcaatttcct tattagcatc aggtatttta tttattaata aatccaaata tccctctgct
    10381 gcatcttcca ttcttttaaa ttcttcatca gtcatgttct ttttaagttg atttttatgt
    10441 tgaatgaagt tagttatatc gccattatct ctaaatgtaa atccatagcc ttgtagcttt
    10501 tgttgtagct ctttttgtgt ttcttgaaga tatgctgttg tttcagataa ttctttagcc
    10561 ttcttggcca ttaactcttt tttcttttct aacaactcta tctgctcatc acctgtagca
    10621 tttttaagta atacatctat cattgctatt tcattattta cttgctctac atctttatac
    10681 atagaagtcc atgtggaatt cttattaagt ttccacattt cttcttgtgc tttccttaat
    10741 tcctcattgt gttctttaat tttattattg tattcttccc attcttgctc acatttaggc
    10801 agttctgtaa atgtaacttt aatatattct tcaaggactt tcttagtttc ttccaactct
    10861 ttttttctgg attcatcttt tttattttct ttgtcaccag actttgaatt taatttgtct
    10921 aattcttttt ctattgatat taacttttct tcatagttag ttagatttcc agtatcatca
    10981 aacttaaagc ccattttctt ttcttttaat ctatttttaa gtctgttact ttcagcttta
    11041 tatccatctt gttgttgttt tattaaatct ttttgttgag caaataattt attttgctct
    11101 tgtaagtatt taatcttctc atcacctgta gcatgttcca tttttttgtc taatagagct
    11161 atttcatttg ttactgctga tatagcattt tctaactctt taaataattc tatgctatat
    11221 tttacagatg agtctattgt gtcattgttt attgtaactt taggtttgat tactttaaaa
    11281 gccgatgtgg ctcttgatac tgctcttgag aataaggatg gttttgttac aggagtttca
    11341 tcacttattg gtgctggttc tgatgttcta ggttgggcta atagattagc tggaattgga
    11401 gttccattag taacagaggt acgttcagtt ttattggtct tatctgaact atttcctcca
    11461 cctccaccac ccgaactatt agtcacttta ttagttacaa atgtagtggt gaatgttttt
    11521 cctgcatagt ttgatttaaa tgactctatt ttcctaataa gccctgatat gttctttgcg
    11581 gcagttgagg ttaatgcact aaatgtagtc tttatagttt taccgccata tcggctaaca
    11641 taactttgta ctttggctaa taatcctgat acattctttg ccccttgtgc agtttctgtt
    11701 ctaaatacaa gtgtagatac ctttaaagct aaatattgtt ttactctagc tataagacct
    11761 gatatatttt ttgcaccttg agaagtttct gcattaagat ttatattata gttattttga
    11821 ccaaactctt cggcttgttg tcttaatgat gctattttac ttgcagtatc ttctaaacca
    11881 atagctccta caactggttc gactttttca tcattaagtt catccatctt ttgcttggta
    11941 ttatctaatt catctttacc ctcattgttt aaagaaagtt ctacaacttt tataggtgat
    12001 ataccttgta taaattcagc agtttgttcg gcttcttctt taccttcagt ttttacatca
    12061 acagttggag tggctgtctt accatttaac tcatcaagtt tttgtttagt actgtctaac
    12121 tcttctttgt tagttacttc gggttttact ttaagtatac aaggttcatt attctcaaaa
    12181 tcaaaaccag gaacttcacc attttctaat ccttgtactt tagcacttac taaaagttca
    12241 gcattagtac catctccatt ttgaacctta tcaatggttt cttcagcttc ttctttgcct
    12301 tcagttttta ctgtaagttc tgcattatga tttccttttg cttcatctaa atctttttta
    12361 gtttcctcta catcttcttt acctttaact tctataccta gcattttctt tatattatca
    12421 ggtagattat ttacattttt tatatcttct aatacacttg gattagcaat ggccattttt
    12481 aaaatagcag tttttttatc tggatacttt tctataagtt gatttacttt atttatctca
    12541 ccaggattat tgactataaa attatatatt aaatcttttt gcccttctgg agtattattg
    12601 taaagttctg tagcactttg tattttttca ggattttcaa ccataaattt aagtatttgt
    12661 ttcttttggt ttccatcttt taagctatca tatagtttga ttccgtcttg caatttatct
    12721 ggattttctg ttgcaaattc taaagctaac tttttagatt tactatcaga tattccatta
    12781 tagaactttt caaaggaatc taattgctct ggatttttta ctataaaatc tattgcgact
    12841 tttctatctt ttttgttcaa tccatcaagt aagtttttga tatttcctat cttatttata
    12901 tttatacttc catcatttat ttttccttgt attgattctg gaagtttatc atataattct
    12961 tttaattttt ctaacttttc attaccttca acaccaacat tatattgaag ccttttattt
    13021 tctggaattt tcttaattgc ttcatctaaa cttttaactc cttgaaaagc atctcctgaa
    13081 ttatttataa aatcaatttt cttttcagca ggaacttttt ctaacgcact atttaaagca
    13141 tttagagatt ccacagaacc agatatacct ttactttcta tagctttttt taatgaatct
    13201 tttgtgatat tctttttatc atcatcactt ttattttcat ttttatctaa gatatcttgt
    13261 aaggtttttt tagcttcttt ggcttctttt tctaactctt ttctatctgt cttaaaatca
    13321 attggtattt taggcctatt ttcttcatta ccatagtaat attcagctaa ttgtttcaac
    13381 tcttctttac ttattaattg attctctcca atttttattg gagtttctat ttcttttgga
    13441 ttaacttttc cattaaatat atcatttaat tgatttgcta catagtcagg aagtttacct
    13501 tcattttgaa ttgtagttga taccttcatc agtatctgtt gctcaaattc agtagcatta
    13561 ttattcttac ctaaaattcc tcttatcatt tcttttactt gcttaggaag gttggtatgt
    13621 gttttacctt cattaatttt aataagggat gatatatcaa tttttccatc aggatcgttt
    13681 atagtttcac taataaagtt atttaagtca tcaaattgac ttttaagttt tattgctatt
    13741 ccattttcat tttcaagttc agcagtagat gaatcataag aagttaaaaa agcatgtaat
    13801 gcttctgctg tagcagtaga ttcaccacga agattctgta atcctttttc caaaagtttc
    13861 gtatctattg gttctttacc agcagctact agcgttttat taatttctct tcctactgca
    13921 tcaactgcat tagcatattc tacagcatct ccaccagctt tataatttgt attagcctct
    13981 tgtaatcttt tattccattg tgctaatttg gggtcattat cgcttattat agaacttaat
    14041 ctatcaaaac ctgacacaag tacatcttgt tctgtaacag atatatcatg ccagttgaag
    14101 acatccatta gctcgttaat ttggctttta acattgtttg tagcattttt ataattagat
    14161 ttttctgcta ttttatcaaa agatacttgt tgtatttctg cctctgcaag attatattca
    14221 tccataagtt cttttgcttc ttgatttttc tcagaaatta aattatttct tttttcataa
    14281 gcttttttat actcttttaa tgatttttca attgcctttg ttttaaatgg tatatcacct
    14341 ttcccaaaag ctttatctaa atcattttta ttatcactta atttttcaat atatttgtca
    14401 ttaagtttat ctatgtcttc agtttgttta ggagctaaat tcatagcatt aagaccagta
    14461 ttgtatcttt cagttttcaa aagttcattt ttttctttaa tagctaattg taatttacct
    14521 atagcttcat ctaaagaacc tgagaatgca agtataggat ttccttcttc atcatatcca
    14581 gcaactaaat caggagaaat atcagctatt tgcttttgta actgtttaaa tctttcaagc
    14641 tcatcagatg ttttattagt cttattgtaa agtttattgt attcttctga taatgctttt
    14701 aaactttgtt gttgttgaga tagtgtgcta atttcacttt tagtattttc tatattttct
    14761 ctatgctttt gagcagatat ttcatatctt ctattccact catcatactt ttgaatgagc
    14821 attgttattc cagatataat cgcacctatt gcaaatccag ataaaagaga actacctatt
    14881 gatgtcgcaa agtttttaaa ctttgacccc atacttttac ctaaattaga tatttcagta
    14941 atagaggttc tacctgctac ttttaattta ttcatagctc ctatagcacc atttacttct
    15001 tgtttaactt tattatatga tgttgctaat tttgatactt tttttatgct ttcatcagtg
    15061 tttttatcag cattattatt tattgtagta gtctgatttt tctttacttt gctttgctct
    15121 cttaattttt ttaacattgc aaaagatgtt attgttgctg gtagtaacat atccatttta
    15181 tctaaagatt ttataacttt atctatacct tcagataaat tgattaatcc atctaacata
    15241 ccatatatca tatcagaatt agctaatgtg ctaaatatag atacccatgt ttcttttaga
    15301 tgatttaatc tacctgctaa actatctaag tattgtgcat tttcagtttc agcactacca
    15361 aactgtaaac cttgcgaaaa ttcacttctt atttctttaa aagtctcata attctttcat
    15421 accctctgtt tccagatatt taaaagggaa tagactatac cactatcttt atataagata
    15481 cctattggta gtcgttgcga cctttcctta ttcataagaa cttaggacta tgtctcagga
    15541 ttacccaatc tttaatcttg ttaccatatc attgtgatta gcaatgccac attttgattt
    15601 ctctagtgct tggtaattaa agctctaagg gttttccctg atattctagg ttttctttat
    15661 atatcactat ataaaggggc tattatgtta acccattagg gattggaata tagcagcttg
    15721 ttgcttcaca ttgttatcgt aaaggctttt tatcctctac ttcttatagt ttcctataag
    15781 gtcagcatat attttctact taatttaagt agtcgaacac tcttgggaat attatcgctt
    15841 ccttctatcg ctcaattcct atgcgttacg gttctaggtg gtattcctag ttacctcggt
    15901 atcaacatat ctaagactta gtcttcaccg attttgctcg ataataattc taaatatcac
    15961 tacttagaac ggcaaataat ttacctgcta ttgcttcagc taatcccttt tgttcatcct
    16021 ctctaagctc atcccattta cttttgacct catctaatac ttcaaccata ttctttaatt
    16081 cgccagtatc tttgttttta tatattgtaa taccagctat ttcttttaat cccatagcag
    16141 ttttatttcg attattatcc tatgggcttt ttatcccata gttcttatac ttttccattg
    16201 tataagtcta gcatacattt tcacctacga ctttacgttt aggtggagga ggctcgtggt
    16261 agaattattg ctctcatttt gtcgctcatc tactatgcgt tacaaacagt attagatatt
    16321 actgctctcg gtattatcct gttaatggag gacttcaccg atacacccct cttataatat
    16381 taaacatttc tgtttaacac gccaatatta tttagctcaa gatttccgtc tttagcattt
    16441 attttcatac ctgctaagtt tattgcaact gattttcaat tgtgttatcc ataaggcttt
    16501 ttatccctat gttcttatac ttcatgtttg tataagttca gcatatattt tcatcttcaa
    16561 cattacttgt taagatggtg aacactcgtg ggaaaattat attctatact attttaaaca
    16621 taaaaatagc ataggttcat ttcctatgcg ttacggtgat taaagtcttt taattcttta
    16681 atttacctcg gtgttaacat ctcagccttc accgattttg ctcacttata atattaatta
    16741 tttctaatta acacgccaat atcacttagc ccattaccta ctttttctgg attttgtcga
    16801 tatcttcaat taggttcgtt aatcctaacc agttctctta tgaacttctt tatatctcta
    16861 taaagacgag actatatctt catcctataa ggatgctctc tgtttcgatt cacttgaatc
    16921 tacataatag tcgttgaacc ttctcccata cggagcttgg atgctgattg tccaatctaa
    16981 ctaattttta aacattcaca tttattgttt ccaattatgt tgtagtttag ttagttctaa
    17041 ggagtttcca gcaattaaga gagtttttaa ctatatatta ctatatagtg cgacctaaat
    17101 taatcgcttc atttgttgct gttatcatac ccactgtcga ttccttcaca tagattcgtt
    17161 acttctatgc agttctctta tgaacttctt tatatctcta taaagacgag actatatctt
    17221 catccactaa ggatgctccc cttttccatt cacttgaatg tacaattagt ctttgaacct
    17281 tcatctgcta aaatgcttgg ttgcggatta tctaatctta agtattttta ctataccttt
    17341 ggagttaccc attgccacta agtatatttc tatcttagtt tagtaactta agctctaaag
    17401 actttcccgc agttaaagga gtttttaact atatattact atatagtgcg acctaaatta
    17461 atcgcctaaa ctaacaccat attcatggag tacactacct ccacgtttcc gtttatcttc
    17521 aattaagttc gctacactta atccgttctc ttatgaactg ctatatgttt ccatacagac
    17581 gagactatat cttcatccta taaggatgct ccccatttcc atatccaata gcttgatatg
    17641 tacggtttat aacctagtcg ttgaacctta ctctattgag tcttggctgc tgattgtccc
    17701 tatcttttag attgttacac ttagtactaa aagcctaaca ggagtttcca gcagttaaag
    17761 gagtttatca ttatatatta ctatataagg cgactaaaat taatcgcttc ggcaacacct
    17821 gctgttccaa ttgcataatt atttcctgca tagtttcaaa ttgttatcct acaagctttt
    17881 tatcttgtag ttcttacatt tcttattcat gtaagttcag catatatttt catcttcagc
    17941 tttatctgtt aagatggagg atactcttgg agatattata ttctatttct agtttcaatc
    18001 tctatgcgtt acattactaa tagctcttta attctaccag ttaactcggt attagcatct
    18061 cagccttcac cgattttacc ctctcataat attagatatt tctatctaac acgccaaaca
    18121 atttagcata tccattgcag cagttaattc tgttgttttc tttgtagtat ttccaacttg
    18181 tacttgcatt tcttttaaag gttcgattct aaatccattt attattgtgt ttactgctga
    18241 agatgcactt tcaacatcca tgtcctttta tacccttgat ttctcaatat ttactgtggg
    18301 aatagactat accatcatcc atttaggacg ctcattgata gtcgttgaga gcttaccata
    18361 ttctttttta aacttaggtc tatctctgct gattgtccaa tccttaatgt tttcagatga
    18421 tgtataagtt tccaaataca tctactaatt aaggctctaa ggagtttcca gcatattctg
    18481 agttcaattg tacattactg tacaaagggg ctattatgtt aacctacatt agcgagtatc
    18541 attgattgtc ttgcagcagc tatactttct tgcatcaaat cgttattaac cagtagcttt
    18601 ttatctactg ctctggaggt ttccctcatt ttcatcagtt ggtcaattcc aactcagttt
    18661 agcatatatt ttcaccttca acttaatgtt aagggctaag gactcttgga gatattatat
    18721 tctatttcta gtttcaatct ctatgcgtta cggttgttaa aactctttaa ttttttaact
    18781 tacctcggta ttaacatatc taagacttag tcttcaccga ttttccttag ttctaattat
    18841 atatttctat ataatgaggc atgaatatac ctccaattcc tgcttgtaaa gatgctgcta
    18901 ttgattcctg aacctgtgat gttgtcattc caacttcttt agctgtttga ttagctttac
    18961 ttcttatttc atccaatttt tgtactgaat ttatatcaac tgggtcagcg acttttttta
    19021 aatctgtcat ggttttatct aattctttat aagttgatac aacccctctt attgcatttt
    19081 gtaattgcat tgctgccata ttgccaagag taaagtaaga aaaactacgt gtaaaatcat
    19141 taaaaaattt atttacacct tttccacttg aacttaatgc agtagttgac ttattcatgt
    19201 tatttatagc tctttgaatt tgttctaatt tactaacttt ttggtctaaa ggtaaattag
    19261 ctaattgcat taattctttt tcaagctttt caattcctga tgttgattgt cctaactcta
    19321 atgcactttg ttttaatctt tttaaatcag atattgcttt gtcaaaatta aattgaaact
    19381 ctgattttgt tttagaaaca ttggtggcac tttgaacttc tttaagcttt aattctaaat
    19441 tttctaaatc agcaatagct ttttttaact catcaccagt aagatttttt aaatcaagac
    19501 tttttaactt ttctgcttga gataataacc cacctaaaac atttttatct gcgtatttat
    19561 ttccactaag ttgtttcaat ttatttataa aaccatcaac acttttttgc attgagttaa
    19621 actgtgattc tactttagaa ttaacttcag gtatttttaa attagccata actgattgga
    19681 ctgattgaaa atcagctaat aattttgata ttcctctaaa tcctaaattt tctaatttta
    19741 tattagataa attatttaat tgctttgata cagaatctat tattgcagat tgtttttcat
    19801 caagaatatg tgtctgcttg aatttagcaa tttgattctg agccttttga atatctcctt
    19861 gaattttttg aaatgtagag gctaaagtac gttgcatatt ttcatatgat gaactatcta
    19921 ttttagcttt tccttgtaac ccttctagtg ctgaaccaac agtttgtatc ttacctaaaa
    19981 cactagataa ttgtttttct aaaactgcat atgattgtac attagtagtc ttagacattt
    20041 gtttttcaat tgaattcttt gtattaatta atttttgata ttctgataat aaactattaa
    20101 cacctgagtt agaaccatta gttgatttac caccaaatag tccattttgt gtctctctgc
    20161 taagtttatt aatttgactt aaaacagttt ctaattgctt taaattattt agagatgagg
    20221 tattcatttc tacatttaat tttatattat tttctttcgc tgaagtctgt aatgatttca
    20281 attgggcttt agctttttca tcatctagtt ctatactggt cttaatttta aattcactca
    20341 ttaaatcaca ctcctttttt tacataaaaa aatagacagt aagtttactg tctattttta
    20401 atacttatag tacattctca tgaacgaggt cagatacttt tgaattagaa tctttcattt
    20461 cttttatttc ctcttcattg tattctaact tttccccttt gtctaatata tctccatata
    20521 tatctaagcc tttatttaag aatttttttg aatttgataa catttcaaaa cctttatcaa
    20581 cctcctcttt tgaaagtgat ttataataat catcagtttt aattttatat ttgatatact
    20641 tatttaatct ttttatattt atatcattta caatctttct tttttctttt aatgtttctt
    20701 tagccttatt taatgcttct tcgtcaaaag taacctttac taccctttta ttagttatat
    20761 cccccttaaa ttccatatta tcattttcat aataaatatc ataaaatatt tcttcacacc
    20821 attcactatc aatctcatca gtaatttttt ccaagttatt ccactctttc tcacacttag
    20881 ctaattcttt ctcagataaa tcatcactac atcctacaaa tcctaatcct aatacactca
    20941 caatcccaat caacaatatt ttcttataca tggtatactc tccctatttt aatacaatct
    21001 atttatttaa ttaattaatt atcttcatct aatgattcca cttgaactcc atacatagct
    21061 acatatcctc gaaattcaaa catttcactt ttaaattttt ttaaatcatc ttgggaatta
    21121 gattttttat atgaattagc gtatttaatc atattgtcat aggaagtttt agttattgta
    21181 aaatattcca acccattatt tactttttca tcatttttga atttttcctt attgttttct
    21241 atatcactca catcaatgtg tttgtcaaca taaaaatcag gtataatttt tttaactttt
    21301 tcataaagat tctcatctaa agggtctata tcattaggaa tagaatctga aataatttta
    21361 tactctttca tggctttact aatcttttca atcatgattt ctgaagtaga tttagccttg
    21421 tttaattcac tatttgattt tattaaattc tctaaagctc tataatttga tagaaattca
    21481 tctgttttag tattattact agcagaataa tctacataac tcccacttgg ttcagttgca
    21541 aaactatgaa aatctgtgta tgaattatag aatttttcta ttgtactcaa aatatcattt
    21601 aaatcctcac taccttcagg aacagatttc aatttactat atatttttag aagttcatca
    21661 tcatattttt ttgacttctc tatttcatca cctttatcca acattgcagc gaaaatagca
    21721 gcatcaatat tagaaccatg tttcttttca taaatatttt ctttccaata agtttgaatg
    21781 gtgtcagcaa tatcttctaa attagcacct gctacataac ttttttcttg aaaatcttta
    21841 atagtcatta tgtattttgc cctagcttct tgttgttttt tttccttgat attgatactg
    21901 gtgaaaatcc ccactatgac catcaatatt ataccagcaa aaatataaat atacttttta
    21961 tttattgatg cttttttact tattacagtc ttttcatcta ccttcttacc acacttagaa
    22021 caaaaaagtt gtccttctaa taacttttct ccacatgatg tacaaaattt agtactatca
    22081 ttttcctgtg atactttgtt ttcatcttca ctcatataaa ttacccccta taatatctgt
    22141 cataagctta cagtagcttt actgtccctc aaagcttatt ctactaccac tataattata
    22201 gcaggaaata tgtagaatta cctctactaa ttgtatatat ttgtaaaata aaggaatata
    22261 gataaaaata ttttaaatgt atgattgtca tacctctgtc atcattgaat tataacaaaa
    22321 aagcctactt aaaggagact tcttatttct ctatctaata tttcttctat attatcaaaa
    22381 tcccaataag gaattcttat aagctttata tttttttctt tagcataatc atttttcatt
    22441 ttgtcatgta ataagttttt attaaatttt tgaccttcaa agtactcctt aaaatgaaat
    22501 tctccatcat actctatcaa aatattataa tccattaaat aaaaatcata tctcaaagga
    22561 tttcctaaat tactaactag agatttgaat tttacttcat ctttatattt aatattatta
    22621 tttataagat actgttctac tcttttttct cctttacttt tagagcattt aggacatata
    22681 cacccctttt ctcctacgtc tgctaatctt cttgaaaatt catttccaca tttaggacat
    22741 ataaatttat acttcttccc actagattgc attactttaa atggacttat actgttactt
    22801 aaactccacg attgagccat atcaaaataa ttatatccaa atgagtcttt aaaattaact
    22861 ttttttccac tacaataagg acatctctct ccaattttaa aatggtcaca acttatcata
    22921 taacttccat gatatttttt ttcctgacat ataatccaaa tttttgtttt attgctaggc
    22981 attaacttat atggattaat tttattttta ttataatccc aatatttgat aaggaaatct
    23041 ttatctgtat tatctatatg atatttagca aaagaatctt ttggactaat cttaaatcct
    23101 ctacaataag ggcatctatt tcctttaaca aaatcagtac aacttattag ataactttca
    23161 tgataatcct ttttgataca tttaatccaa actctaatat cttctttttc agaattttta
    23221 gagtttggat ttttccatat attgtaaggg ctaactttat ttttctcaaa atcccaatat
    23281 ttttctattg gttcacctag ctctttttca atataatgtg caaaactgtt ttcatatgaa
    23341 ccacaacact tcggacatct ttgcttcata tttataaaag catttgcact aacaacatat
    23401 atacttttac aatacttatg ttttatctta atacaaactg ttctagattt tttcccgttt
    23461 tctaatatat cccctttaaa gtaactatct atgtattcat aatcagaatc tttctcaacc
    23521 tctattttat gcttctcttt aataatactg tatttacatt tattacacct taaagcacct
    23581 ttatctttca ctgttgtcca agttctcttt tctattatat ttccacattc acatttccat
    23641 ttatgattaa aattatttcc accatatatt tcatcaacca actctaaatc aacatcattt
    23701 tccttcaata acctattaac ttcatctcta cgcataccct tcttcaactt aacatcttta
    23761 accacaatct caccttctcc aaaaggaagg taacctaaat caccttccat ataattactt
    23821 aaaaaataca gctacaaaag aatacactat acccacaact ccatcatcca ataaatcaat
    23881 taatcttttc aattcatctc tcatatcatt cacctaacaa acttcttcaa gttctataat
    23941 aggtaatact ccaatagatt ttaatttctt atatatcatc aatcttcctt tttgagtcca
    24001 tttagtattc attttaatgt caggagttcc atcagccttt acaatatcta ttgtttcaga
    24061 atgagtatat cctttagctt gataatcgct atataataac cattgtccac tctgtttgaa
    24121 ttgaattcct aattcatgta gtttcttatt catagcttta ccactcattc cataatcttt
    24181 agctatctga gttatagtta ccaatccttt atttctaagt atcaaatctg tatagtctgc
    24241 ttttggttta agttcaccta tgatttgttg ttgtttatta tttgttactt ctaaagcttt
    24301 tctcttttct ctctcttctt ttaggtctgt ggcaagtctt atgattgtgt ctggattaga
    24361 taatatttct tctatctttg tgtcagttag atatgctcca tgtttacgaa tagttggtat
    24421 tacatctgta gccaaccatt tttgaaattt taatgctgct tcatttttag ctttcattcc
    24481 tagcatgtaa aacaatgatt ctggtatgta atcatctttc gcccacaagt ggtcgaagcc
    24541 aaactcctta gaaaactcat tcattctttt ccatcttact gatttatatt ctttttcacc
    24601 ttttctctct accttgcacc atccaaatcc tatagctgtg tcttcagcat taatacctat
    24661 acttccatct tcgaagttta tagttctaac acttgtacct aactgttcat ttgtaaatac
    24721 ttcatttctt ttttcattac tcatacacac atctcccttt tatttgaatt tttattaaga
    24781 gactgatata ataaaattag tgttattgat tattatatca atctaattaa aggttctact
    24841 gcttggcaaa gtttgtagaa tctttttaat ttcacacatt tagctgtttt ataaccttta
    24901 tcgtgtgatt tatttatatg atatatcatt cattttgtgt tgtcaagatt tattatatta
    24961 tgtatcacct tttttgtggt ataatacaag aaaggagtga tatcatgttt ggagaaagat
    25021 tgaaagaatt aaggcttaaa aaaaaattaa aacaatctga attaggtaaa gagataggta
    25081 tttctgcaag cacaataggt atgtatgaac aaggtagaag atttgcagac caatctacat
    25141 taataaaatt agcacaatat tttaatgtta ccactgatta tcttttaggg ttttctaaga
    25201 caaactactc agttaatgca actatagctg gattatcacc tattcaagag gatgaaatta
    25261 taactaatga atccataaat gaaacaataa aatctaaaca aaaagatgtt cttataaaaa
    25321 agttatattc agaatctcag aaattaaata acaatgaatt ggaagcaatt attaatataa
    25381 ttaatatctt taataataag aaagaggatg attaaaatac attcttcttt tttattttca
    25441 gcaatttcat atattctcaa tattcaactt aataaaccat acaaacaact aatatcttac
    25501 tcaatatatc tcgaaacaaa acctctatta acctaaaaat aatattatac taaaatcaag
    25561 acaatacatg ataacattcg tatatatttt gttatcttaa ttttagtata atagcatcat
    25621 atcaaaatgt caacaaaata tatacatttg ttgtcattta tgttaaactt atgaaaaagg
    25681 agctgatatt atgggaattg gagagaatat aaaagaattt agactagata agggattaac
    25741 ccaaaatgac ctagctaaaa aagcaggaat atcaagagtt gctttgggca attatgaaag
    25801 agaagaaaga gttcctagca ttgagattct aaaaaaaata gcaatagcct tagatacatc
    25861 tgaggaaata attatgaaac tacaagatta cactcttgaa gaaatttaca gattaaaaat
    25921 aatagacaaa aaatatgttc cattaactaa acatcaatac cgttttctaa ctgagattat
    25981 agacaaatgt acatcaaaca tattaaactc agcttcttca gtagattatt atgatagagc
    26041 ttatatgtat atatttttaa ataactatga agatgcaatc aaggactaca caaaagctat
    26101 tcaattaaat cctaaatttg catttgcata tttagacagg ggaaatcttt atgcatttct
    26161 tgggcataaa caaaatgcat ttaatgatta cgataaggct tttgaagtag cacccaatcg
    26221 tgatgttaaa tgtactatat ttaatagtag ttctgaagta ttatataaaa cattgggaga
    26281 gcatatacct aaaaatgata tagattctta tatccttgaa aaaaaagaaa tctattttaa
    26341 gaaaactgat tcacctctag ccaataaaat aaacctacta aatgatgatt gtatagacct
    26401 aatctccaat ctagtagata aactaataaa agataaaaac aatctaagat aagtatctca
    26461 acaactagag gtgcttattt ttatttctaa tatataaaca taattacctt cgtcatacat
    26521 ctgtcatcgt ttgaataact atcgtacaac aatatttgat tatatctaaa atttataaaa
    26581 ttaaaaacat aataaaagcc tacttcaaag tagactttct attttatatc aaaaatataa
    26641 cttatctgtt ttttaaatat ttataaattt taaatttaag gctagttagg agctacggaa
    26701 tttaattcac atgataaacc attccagtaa atcatctact ctcaagtaat agctcctcaa
    26761 tcccatcaca ctcaaacatt aaaataagct cttatagtag ctaaacacca aacaaaatgt
    26821 tcctctcagg aacacttgtt gaaattccaa tactttcaac cataaaaatc aacctcttat
    26881 gttcgattct aagcaattaa ggtattatat atctttcaat atttccaagt gtattatact
    26941 tgtcacacac atgtcatcat tgaaataact acaacataaa tgattcaatt taaaaaacaa
    27001 tctaaattga ttatctttaa tatttattaa tacatcttga tatttatatt acaatagaat
    27061 aaaccttcta ttatcttata ataataaaag gtttattcta tctccacacc ttaaaggtgt
    27121 actatggcca ctcgccttat ctatattaat ataataataa acgtcttata aattttacaa
    27181 tttaatctct aaaacctatc ttataactta tacattcatc atctacaaaa taaattatat
    27241 cattgaacat aaagaagcta taatcatttt tctttgcctg attagccatt tttattatat
    27301 ctccagtcca aatatcatca tcataattat atattaaatg ttttggaata ttgactttat
    27361 tatttgtaaa actatctttt tttaattctt acttttctat tatcttacta caagattccc
    27421 taaaatgtat attaggataa tctaattttc ttgtaacttc taatagttcc tcgatatatt
    27481 caaatttaaa tgttaattga aacaattata cctctctctt tatatcaata tacttgtata
    27541 tatttttgca tcaaccaatg tactagaaca ttgagaaaca attctaaacc gcttatctaa
    27601 gcaaatcaac taaactacct atctaggcta aattgttcac tatatggtag aacatcaccg
    27661 ctttttaatt ttccacttat agtaggaagt caacctatat ctattctact ggtacaccta
    27721 gtccattcat tgtagactta aaacataaag gaatctcagt tcttacttta gtctcacttt
    27781 cagtcattat atttgttttt ggcctaaaat taggttcttt tctagtacca cctgcacccc
    27841 aaacttttcc ttcttctaat ctttccattg ggaatgctgg gtttttacta aatgcatcaa
    27901 accattttcc attattctgt atctctgaag taacagaatt agaagaaagt tctgtgacat
    27961 ctactgcctc taatagttgt cctgttctac cttcccataa atcaggatgc catacacctt
    28021 ttggagtata tccagaataa acttgcctat ctacctcttc tttcattatc tctttcatct
    28081 ttttaccaac ttcaggcata gatgttttaa tttgattttt agtataagca actaattcat
    28141 ctaaagactt aaataccatc taattcacta ccttatcttt gatattattc atcttttcga
    28201 tttcctcagt ctctttgatt accctatctt gtaatatttc attttttaac tgacctaatt
    28261 taacctcagt attcattaat aaagatgcag ttaattcatt ttctatttga cctatgtaat
    28321 acatcacagt ttctaactca ctagatatat taccatctat cacatcttgt atatcaatat
    28381 ttaactctat attagttaac ttatcaacta acattttaag aatatcttct tgagacaatg
    28441 ctaaatcagg attctccata ccaatccata ttttatttaa tatttcttct ttagtctcct
    28501 tatcaggatt tctaacttct atatattcct ctttaccact ctcatctaca attggtatta
    28561 tagccctaac ttcatcttgt attaatttat ctacttgtat ttttcccata tctatctctc
    28621 ctttatttta ttattggata tttacactta atagaaactt tacaatttcc agtaaacttc
    28681 aatatattta cacctcttct taacctaatc cactttctat taacaatatt aaacctattc
    28741 tcaccagagc tatttaaaac agtatagtat aaattatcta ttgttatttt ttcatttaat
    28801 cctaatccac ttataactaa actgtcttct tcattatcgc tgatagtgga gtttctaatt
    28861 gttatatctc cttcttttag acattcaata tcaattactg gagcatattc ttcatctaca
    28921 ttagacacat tatttagttt aatttccctt gtatccttga cagttataac tttttgaaag
    28981 cttctataag cataatttgt atatggttga aactctattt ctaatacacc ttttttatct
    29041 tttgtatatc tttttattat ctttctcgct ttgaaatagt atatttcttc tatattgtca
    29101 taactgataa aaggtttaaa ttctctctgc caaaaccaat tgcatatgtt ttctagcgta
    29161 tagtcatccc actcataagc ctccccatgt tcgctacata aacagaattg taatgttata
    29221 ggttctatag tagtttcatc ttcactgtaa caaggatttt tattaaaact gctatcagat
    29281 ttaattgctt cttcataagg caatccatat tcattcagta tttcatcatc acctaatgta
    29341 actaatttga tgttcataga ttcacttgaa acattatcaa ataaaaactt atcatctaaa
    29401 aataccaatt tatcaccttc tttcattttt ttaagttcaa atccgattaa aaccacattt
    29461 aaacgtaaaa aaataatccc tactcttttg agtagggtaa tttattattt cttagattta
    29521 gtatcttttt cgtctattaa ttcagcacct aacatattcg ctatcataac tggagtgaca
    29581 tcttcttttc caataaatgc aaattctaca taattaccat ttgaatctgg taaaacatct
    29641 aatgtactat taaaaccact agggttttct gctgtaaatc ctaactctat acttctttgt
    29701 gctttgcaat ttggtatagt catatgtaat accataagtt tactttctgc tgtttttacg
    29761 ctaacatcag cataaatagt atagttaggg gcagtagaat cattgttaac tgttatagtc
    29821 ttagcttttg gtaattcagt taaataaaac acagctactc tatctcctgc attaaaagaa
    29881 gagcttgaaa ttgttatttc tgtattttcc ccacttattg ctgaagtaaa ttgtaattcc
    29941 tcaattaatg atattccatc atcttcaaca ctatgaacag ttaaacttcc ttgaagtggt
    30001 tttactcctt ttaaagttac tttttttgca ccatcagaag ttaataactc tcttttccct
    30061 acttttgcag ttttttcaac tacatcagaa gctaatatca tagccaattg tggtaactgt
    30121 attacctctt gttctattgt aattgatgat gttatcgcac catcgaaagc tattgcattt
    30181 gctcctttag ctttagcata tatactttct gactctaatt taaaagtaaa agtatttggg
    30241 tcttctgtgt aaaaaatagg tttctttgtt attgcattta caattattgc attagctgca
    30301 tcttttatcg aaaatctcat attatattat catcctctca ttttttttgc ataaaaatag
    30361 cactgctatt taacagtgct actcaatatc tttatttttt tagaccaatg ctctatttcg
    30421 gcatctgttt gatacttaaa tgaccatgca aagcctaaac tttcattata attcttaaat
    30481 tgtatcttag tcttataagt attaattaat tgccaatagg tcatttttaa tatttcgttt
    30541 attgatacat atccatctat attttgaatt atgtttatat aatcacataa atggtatgct
    30601 tccttttctc tttttcttct ttccttctct ttgaaatgtg caattaactt tttttctctt
    30661 tcagaaccag tattttcaat ccattcatct tcatcatctt taaataaatt ctttttatca
    30721 atacaaaaca tctctaaaat taaatctgat attagtccaa aatcctctct acttataaaa
    30781 gcatttttct ttttatcatc aaatttaatt aaaataccaa ttttaatatt attcatatac
    30841 tcaatatttc taatatcagt tttatataat atagataaag atttttttat cttatcacta
    30901 agtgattcag ttttttttga agaatcttta tctgattcca aaatatttaa atctaaaata
    30961 ggtatagcat catatttata tttcaagtta aaaaattcat tattacatag ctgagaataa
    31021 tttttctcaa gtattaaaaa agggttaact atctctaaat tagctatatc cagtttaata
    31081 agctcttcaa tagtaggttg atatacagtt cctaatattt cacttagttt aattggtaat
    31141 cctgtaatat aataattctc taacattaca tatcaccaaa actttcagca gatacagtta
    31201 taacaaatcc attataatca gtacttaaat tatacatttg atatacattt tctaacttaa
    31261 tcttacctat accagctatt tcctcatttt gagttataat atcaactata caacatacta
    31321 atgcaacatc tcttaagcca tttgctgtat tttggcattc atcatgacaa acaacaccaa
    31381 tttctatctt tgttttttta atttctctac ttccagtagt acatggtcta tactctgcca
    31441 ttattataaa aactgaaata tctgcttctt ttaatacttt ttctactctt ctatttagaa
    31501 atactttctt gtttctaagc tctttaactg gattttttat atcaggcatg gataaaatat
    31561 ctttttctga ttcatcatta taaatcataa atttagcgaa gtcttgattt gacattagag
    31621 ttgttcctat attacttatc atcttgttag ggaaatccat ataaactcat atatctaccc
    31681 ccttagtgtt atttttttag tatctattat ttcatttgtt tctttgtctt tagccaatag
    31741 caaggcttct aagccagaat acttagccaa tgtattaacc tgtatgacac atttttcctc
    31801 atcttgataa attatatcac aatatctgta ttgcttgtct aagagccatt taactccttg
    31861 agaatggtct attctgtatt cattttcttc acctagatta ataaatccat cccctataat
    31921 cttactgaaa ggaataatta cactatcatt ttcagataaa tcattccaag ctatattatt
    31981 tactgtatca tcttttgaaa tcaatgcatc ttgtaaacaa atagctttaa taagaccatc
    32041 tgctcctgtt tctctgccat tatactcaaa gtcattaata ctagtaactc taaatacagt
    32101 tttaccagtt aacattattc tagtgtcaat atctatagtt ttagtgacag gattacttcc
    32161 aaaataaagt tgtctttttg tatctgagat agaagtatat ttattatctg ccataccatc
    32221 agagtataag gttaaatttt caacagctat agggatttta tacatctgac ctttatattt
    32281 ataattaaaa aactgattgc atctttttat tatgaaatgt ttatatgtat ccatttcttt
    32341 atgttcttta aatatagtta accaataaca attatcatag aaaagatagc aacccacatc
    32401 tacatttaaa tcagattcaa ctaatagatt tttttcatct aaagctcttt tatcattatt
    32461 agttatatca ttaattgcaa ctacagcttt tttagtatct tcatttatac aaacttcatc
    32521 tatatcggtt ataggaattt caatacaagt tggtgtttct tttaaatatt tcttaaaact
    32581 taatcttagt tgtaatattc ttttttcttt aggtgttgaa caaccaagtc caattctttt
    32641 ttttatatca cttaagtaac tcatgctagt tccatccttc aaaatttttt agcatgtatc
    32701 tttgttgata tttattaaat ctagttctca attgttcttc tcttgcttga agtttagcca
    32761 acatattagc acctgattta gcattataat cagaatcagt tagcatctgt tttaaatttt
    32821 cttctctatt tatcttaggt tgtaaccaat gtatttgcat accatatgat aatattttaa
    32881 tttcctctaa atctaaatca gatactattt ctcctaaata tttgtatttg aaaattatct
    32941 cttctgtagt ttctacaaca aaacttatta taaatttttc atcttcaaaa cttattgtat
    33001 aatctttatc taattcgtat tcttcttttg tatcttttcc catcaaagtt atctctgcat
    33061 ttttattact ataattagat gtataactta gttgagcagc aggaataatt tcctcgcctt
    33121 tttcatcaac atattttata gtcaaatctt ttttacattg attaaaatca actatagcat
    33181 cttctaaata actgtacatc attttttcta ttatttcatt tgatagtaat aacatctctt
    33241 catcattaat agcatccaaa aactttttat atatttttac aagtggagta cccaaatatt
    33301 ccaccacctt taactaaaca agtacaaatc ttctattcca attcttttag ctaatagttt
    33361 agcctttagc ccagaatcaa actttttctt tttatgtaaa tctattgccc tctcagctaa
    33421 tcttcttgtt aactcaacat tatttttatt taatattttt tcaaaagaat ttatatcaac
    33481 tttatcagat aagaaatatt ctaaatagtc ttcattagga tttcttatat gttcatataa
    33541 actattcaat cctagaaaat ctattatgtc cattggggta cattcaacat ctaaagaatc
    33601 tacatccgaa attattaatc tatgttcttc aaaaaaacct ggacttcttc tcactattct
    33661 ataaagagta tctaaatcta taaattcagt tgaatctttt gaacctactt tagataaaga
    33721 aaaaatttgt tgtttttctc ttgggtcaat ataatcaaca tcccatgtgg atatattttt
    33781 tatttctata tctatttcct gttttttttg ttttagcttt ctttgtagtt ctaatcggtt
    33841 taatctagct tcttttttat ttaaagtaga ctttatatct ttttctaaag tattagcttt
    33901 gacttctgtt ttagaaatat tttctttttt agctcttgcc ataataatct ccctcccata
    33961 aaaaaagaag gtggataacc accttctaat tcaattattg tattttataa actccaaatt
    34021 tagcagcagt tacaactcct aaatgtattt tttgagccat aaaatattct atttgttggt
    34081 catctctttc tttttcatca gtattttcta tgaccaatac atctccttca gtaccaagtt
    34141 tgattatctt ctctccatca ggtattacat agatagcctt attatctaaa gcccactctt
    34201 taccttctgc tgcattttca tcataagcat ttggtaaact aagtacttta tatccacctc
    34261 taaatgtttg tataaaacca tattttcttt tttcttcttt ttctaaatca gtttgaacac
    34321 cttctaaatt acttaatgca agtggagtac catatataat aggcttttta ccagtagccc
    34381 cttctacctt agctatcaat tccaataata ctttgtctga attacctgta gcttgaaact
    34441 tagcatttat tgttgaatat gcaccttcaa atgtttttgc tattcttcta gcaatatcat
    34501 aatgatatga atctgatacc ctattcacaa atacaaccca atttattctt cctgttataa
    34561 atctctctgc ttcttcatat atagcaacag ataatttaaa tgctgttgta ggaacttttt
    34621 tgttaagtaa tctttgtctt cttgtactat taacaccatc tgctatattt gcaactttaa
    34681 ataaattagt attttcaaca gtaaattctt ttttatctcc taaatcaaaa ttctcaaaat
    34741 cacaaaatgg actaaatact tcctcattta atctattaat atcatcagat attaattctg
    34801 atattaaatt ataaaatccc catttatttt cttggaattt ataataatta tatttttctc
    34861 ctcccatcat ttcttttaat ttattcttta ttgcactttc agcatctcta tatgttattg
    34921 tgtttccatc tctatctgta tattctctaa atgtatctgt atatagctca tgagctattc
    34981 tctttaaagt ttttgctgtt gccattaatt tacacacttc cttttctatt tttttgcata
    35041 aaaaaataac agttatagac tgttatctcc tgtttgttaa ttttatttga ttttataaaa
    35101 cttttattgt aaccgaatct tgaccttcat agttccataa ttctaatact tctgctactg
    35161 gagttttagc atctgctgta ggcaattttt caaactgaaa cgaatcagat tttaattgta
    35221 acttgtcttt tactgcaata cttttatctg ctatatgtaa tagagatata gttacctcat
    35281 ctcctttttt tagtcgtcta catctacata tctgaccagg agaaacttcg taatctcttt
    35341 catctaatct ttcatcatac atagttgctg ggtcatcaac tataccccaa tctaatgtgt
    35401 catttgtaac tttatatatt ttatgggtat ccctaccata ctccgtgtct tcaagttctc
    35461 caatatttat tacagcacca ttttctaaat ttattggatt ggtatcctct gatggaacaa
    35521 tatatttacc tgttaaaaca tcaggatttt ttactttatc tgtttgtatt attgctttat
    35581 ctttcttcat gtaaattcct cctattattc tttatctgaa tattttttta taaaagatat
    35641 ttcttctggt tcactaaaac tatcattatt attacttata ggtattttta tcgtattatt
    35701 tttatcatca tttatactaa aattttcctt ttcgctttgt ttattgctaa gagtttttaa
    35761 tgcaaatatg taacccaatt ctttcttgta ttcatctaat gtaatttcct tattataagc
    35821 ttttattttt atatctttaa tttcatcttc tgctaattca aatttttgag ctacatcatc
    35881 aactttagat ttaaataact caaattcttt ttcttctttg aatgcctgta atggctctaa
    35941 actggctttt aaattagata tttctttttg cttttcagta aatttctcta ttatttcttt
    36001 ttttaatttt tcttgtctac taaaaacctg tactatttct ccttcttgtt tttccctcca
    36061 agtttgtata tatgattttt tattttcata atccaaaatg acagcatctt cattaactat
    36121 atatggaatg ccataataat acccccagtt aatttcatct tcaactatag caatattctc
    36181 ttctggtatt aaatcattat aataaaaagc ttgtacttca actttttcac cataccaatt
    36241 ctcagtttcg actttgtatt cttttaactt atttcttatt tcagtactaa gattactcac
    36301 tgctaaagca tatccttcag aatttttatt aagtttttga ttcttatttg gcaaattctt
    36361 ttccccctct ccatgttcta atgcataaat ttctgagcat atttttgata tttcttcttt
    36421 ataattaatt tttcttgaaa atttagttaa acaacaattt gaacccatag ctccctttac
    36481 atcagaacca agacatgtaa ttccatgata tacatagtct tttatattat aaaatccatc
    36541 tgaatcatct aattgataat catttatatc tatttccatt gaaatttcta tttcatcaga
    36601 atccataatt atatcgtaag catgattgct atatgattta tatatatatc ctttacattt
    36661 aacatacttc tgcccatcaa tttcaacttt ttcatattta tttgtttctg gtataacgcc
    36721 aattattcgt tcttcataat attcttctaa tataaaccct tcatctgttt gaactacttg
    36781 tgttaaaaca ttatgaccat caaagtcata ttttccatct tcatttattt ttacatatcc
    36841 tagaattgga acatttttta atgttggttc agctttatca atagcttctt cataaaattt
    36901 agttccattt ggatttgtac cttcatgcat aacatagata tacactggca aaagattact
    36961 atttagttct tccaattttt ttttatctga ataatgtttt aaattacaag gtaattttaa
    37021 tatttttatt tttttcacct ccttttaaaa acgtattttg ctagtaaata taaatctttt
    37081 catttcttca ttactgaaat tatttattaa ttttgattta ttttcaaaaa catataaagt
    37141 cttattattc aacttgtttt cacatataaa acgatatcct ttttccatta ataatttctt
    37201 atcatcttca gaaaaagcat atataaactt actcataatt aatcattctc cccatttcct
    37261 tttgcttgag tagaattacc attatctgtc cctatatcag atttatctgg tctaccactg
    37321 ctttgagtat gagatgtttg ttgaggtatt aaaaactcat caaatcccat taatttttct
    37381 gcttgtaaag tgtttatagc ttgaagtgga gtaaacccag tacttgcaat aaacaagctt
    37441 ttactccctc cgtatcccat atcttctcga tactgtttgg ataaattcat tttattaaag
    37501 tgagtagttc caataaactt catattaaat aaatttgcct tcttgtcttt atttagttca
    37561 aagttaaccc aattttctat catttgttga atcctcatag gaattaagct atctgcaata
    37621 ataccattta agatagattc attatttatc ttgtcagagt taagtaaggc tgtatttatt
    37681 ccagcgttat caaatatgaa ctcttttgct tcttttacat aatcatttat tttagatttt
    37741 ccatctgaaa gagtatgtgt ttccaactct aaaggatttg tagttattgc tgttcctttt
    37801 ggtaaatttg cttttgttga attgtggtat atgggaatta aattaaggtc tactagtggt
    37861 tcatcatttt tgccaaatgg tatcttttga tgtattaatt taatactttc tattactgca
    37921 tttgtgcttt ttaaatcttt catatcttca agctccatta aatcgtcaaa tataaagcaa
    37981 aaaaatggaa ctcctttcac tgaatctaaa tcataattaa atgctactgc tttatcactt
    38041 aattgaaaat atgtattatc aattaattct tctcgtgtaa gtaaattaga tttatatttt
    38101 ttatatatgt ttttaatttc ttctggaaag gatattatgt ttttgtcagt aagttttttt
    38161 atatttatag cgtatctcat tacattattt tctatcattg ttatcataca aaattttgct
    38221 ggtatctgtt gtaaaaccat ccctgaatta tcttctatct tatataaata aacctctcct
    38281 aattccaata ctttttcggt tatccaagct gatgtaaatt ttatattata tttttctaat
    38341 tgactcgccg aattcatata tgcttttgtt attttttctt ttgtttttaa tctacttatg
    38401 tcttttgggt ataagatatg gtcaaaagta ttcatattag atattaaatt taaaactctt
    38461 ctatatgtac cagatgtagc ttttaatatc atacttgcat cctgaagtat atctgtattt
    38521 gaaatttgat atggattgga gagagctttt cttaatttgt ctctgcttat acttcttaac
    38581 ttatacattt ctgttgctaa tgttgtggat tcttttactg aggcatattt tccaatttga
    38641 gcatttagca tattgatttg attttgtgta gaattgttgt ctttagactt gcttttatta
    38701 ttattttttt tgcccactta ttcacctcat ctcttaccaa taaatcataa cagttttttg
    38761 attatttcta ttttttcttt tctctttttc ttccagataa tctgctaaga aattcgtata
    38821 agccaaacta gaatatctat cttttcttgc tccacttttt tcctttattt taatttttcc
    38881 tccatcagca ctatattcta aatttattaa ctcattcact aaagccgttg tttgaagata
    38941 aggggcaata tattttgctt ctaattctgc ctttttatct atttcatctg cctttatcat
    39001 ttctttttct aatatcattt cttttgcttc aatatcactt ataggaagtt ctaaggttct
    39061 atttgtaagg acatctctta aagacatagc cattttatga ttttcatcag cgtaagcttt
    39121 catggagtat acaacaggaa gtgatttttt tgctagtgac ttgtcaacag tattatcttc
    39181 attaaaacat gtgtaagcat cataccattc atctctatct gaatcataat ttgctttctg
    39241 tatatagctc catacagttg ttcctatacc ttgtgtatct aatatcatat aatctgcctg
    39301 aaaatctgaa aataattgtt ttaatcttat tgcttgtttt tcagcttcca taccattata
    39361 agcttctata tggacaacac atcttttata tgtgccttca tttggaagca tcctcatcaa
    39421 agtataaata gaattatcat tcttaactcc tctggccaaa gctatatctg caccaataat
    39481 ccgtatttct ccttttatct tattaagatg atatttctta tctctttttt tcttattttc
    39541 aagatactct atatcactag gaggataaaa catactttcc aataccctac atggattcac
    39601 ccaagatgac ttgaagaaag catcatctga ttcattataa aacactccac aatactccat
    39661 catgaaagaa gcatcactca tactctcttt tttcatttct ttaagtattc tttttttagt
    39721 taatagtcca tgttctaaag agcaagtgaa tggaatacta catgcaaaca tatcttcacc
    39781 actaagcata ccttttactg tttccataaa ctttttataa ctccaatgct gagaatacca
    39841 gcctgaacta agataaattt ctttattctc ttcttgcata tatctatatt taggattctg
    39901 taaatatcct ggttgtcttg ggtttgttaa aaatggaact aaaacatcat tcacagttcc
    39961 ttcatccacc attctatatt catcaacaat aagaacatta gctctgtaac ctcttgcatc
    40021 atcattagat acaacagctt ctattctact tccattccaa aaatctatag atacttcttg
    40081 tgcatttact tggaaacttt taatttctct tttcagagta tctgatttta aatataaatc
    40141 acctaaaatt ttacttgtaa taagtaattt tgcttgtttc tttctttttg ctgctattaa
    40201 aacacaagta cctggtttta atacagctat aacacaacaa aacactccta gaagccatga
    40261 ttttcccagt ccacgagaag caataaatac aaattggtca gacttcatca tcatataaat
    40321 taatatctgt tgaaataaat gtagattaaa tccaaagtaa tccatacaga atctatgtgg
    40381 atttgcgaga taaaaagcac cccaatactc tcctatactc tctagtagat ttcgggatga
    40441 atcattagat gacagctctc ttttttcata tttagtcctc atttttatta ctttctaaaa
    40501 tgtctcttac atcatcttta aatttaatat ctttatcgcc atcatttatc gaatacttac
    40561 ctgtagctaa acctaatgca acagcaagtg gttttttcat atgtttattt acataagctt
    40621 caaatccatc tacatcttca tattccttta acctatcctt tacaggttga tttttttcat
    40681 atatcatcat tttcattcca aatgtatcat cttgcgaatc tccagctttc tttttctgac
    40741 taggctttat ttcagcatct tgcattttac ttgatactaa tttagataaa tcattaaaag
    40801 ctttcatatc tcccttttct cttgatttat ctttttctaa ttcaataata caaatctctt
    40861 caaacaattt tctttcagtt aatgactctg gttcataata ttctttatat tccttatatt
    40921 ttctttctag gattttatac tcataatcat caaaacctct tccccatttt aatttaattt
    40981 catcatttat gactatattt ttatcattat tatttcctgt atatatttct ttcaataaat
    41041 tatctaaaga tgtctttccc ttatattttg aatttctatt aataattttc atatattcat
    41101 ctattaattc atctttattt tttttatctg aaagttcttt agctaatttt tcatcaaaat
    41161 aaacatcaaa gttgaaacat atatgtttta gtgcctttac aagttctcca ttgtaacacc
    41221 catttaatag taaaaaccta gctctaaaac attctttgca tactggaagt cttttattta
    41281 aagtaaatat ttcactatga cttttccaaa aatatctatc tgtatatttt tcttcaccac
    41341 aattcaaaca ctttgtttta ctaattgcca ttcataacat caccaccatt aaaaaagacc
    41401 actagagaat taaatctcta gtggttaata gggagattca actcaattaa gagctgaatt
    41461 aaatttacaa tataaactta actcagctct taataaataa cttatatctt acttaaattt
    41521 atattgtaag tacatattct cccactttca ttaaatatca ttaatttttg cattgcgtgt
    41581 gaacttaatc ttagattctt agcataagaa tccgttccgg agaaacttcc atttacacat
    41641 atttcacttt ctccataatt atcttcctta caattgtgta gatgagccat aaagatataa
    41701 tcaggaatta attttataag agaggtcagt ttaggaattg ctgtagacat tttatcttta
    41761 tctccatgca ctccaaaaca agtatttcca catattctag ttactattat gtcattatca
    41821 tatatgttct cttgaaatat tacattatct aaacttttaa ttcttagttt tatatattca
    41881 tctattaata gtgtaaaatt atctttatct aaattttcat cttttttagg aagaactcta
    41941 tcatgatttc cgccaattga ataaacaata attttatcaa tttctttaga taattcatat
    42001 ataaattcac taagtatttc agatacttct ataatttgtt caactatatt ttctctattt
    42061 tctaatctta tagaattatg tatgtggcca gagattaaat ctccaagtag cattacatgc
    42121 agtctattaa tcttatgcaa cttactatat tcgataattt tatcttttaa atattgtatt
    42181 cttatcttga atatttttga attatatcta ttaaatgcat taatagtctc taatccataa
    42241 tgtatgtctg aaattagcat aatagcctct tgatttgaag attcatagca tttgtaacta
    42301 gaatcactta gaaacggctt atgaagtgat atatcatcta ttttattatt aaataaatca
    42361 attatattat ccattcttga atattctctt attttcttat ttactaatga cctttcatca
    42421 gatagttgta cttttatctt ttttaactct aatattttct catttatttt atcaatctct
    42481 tcttgagtag aattattctg tatcttagaa ttaagataat caaaagcctc tttcaaccca
    42541 taagaaacct tcctacaatg gtctggagaa cattctaacc ccaatagttc aactatctct
    42601 ccccaatcca aatctatctc tttattaacc tttgctagtc gtattctaat cctatagtca
    42661 actagtgact catcttcttt tttcttcaat atgtcttcca tatttaatat ctccctgttt
    42721 atcacataaa tatcattatc aatatattaa gtaaaaaata aatagtccaa tattgattta
    42781 tttaactatt gtttcactta atcttatagt tatatttaaa tctgtttttc caatatactg
    42841 gtcaaaaaca tcactagata acacttcatt atcgccattt tttgtatgct ctataaattt
    42901 ataagtacca tcttcttgtt ttattacttc acatttttct aaaatcgttc ctttattatt
    42961 tttcatatat tttctccttt atttttatgt tatctataac tctactggga atatatttat
    43021 ttgcaaatta ggcagccttg tagctaacaa tagctaatat aagttaacgc atttagcatt
    43081 tatcaaggat tttctataac ttttttagtt tatttttact attttaataa tttaatcttt
    43141 tatccttgag attttatatt tgaattttta actttaagct ttatatctat tgttctcttt
    43201 tatacagtac aatcgtttcg tttgtatgta cccatcactt ttatataagc agtttgattt
    43261 ttaacccaaa atcaaatggg aacttataat ttatgttatt cgcctaacct gcaaattaaa
    43321 aaatagtcca attaaggact attttaaatc tacatctata attgtaagtg catttgatgt
    43381 actcaatgca aaatcaactt ctttttcaaa ttcttctatt tcatccctta acttttctat
    43441 tttctctctt acttttaaag ggtctactaa agaccattct tgttgttttt tataaaatga
    43501 aataagttct gctccatctt tacttttttc tttttcctca aacattctgt ctgtattttt
    43561 ttgcacttct tcatttctac gatttacttc agacatcata caatcatagt tactaaccat
    43621 ttgtctcaac aaggatttat ctaattctat actatttttt ctttttattg cttctaaaac
    43681 tgtataattt ttattaccaa ttttaacatt tgttatagca ttagagatat taattgcttt
    43741 atctaatgcg ttataattat atattaaatc ttctatttgt tggtatttac ttgaaacttc
    43801 tacattaaaa tcttctacac ttgtttttgt ttcatataca tttttatcag agcctttttt
    43861 agtacctaca attttaaaat tactaatgca tctatctatc ttatcttcta ataacttctt
    43921 ttctgctaaa gctttatgaa cattatattt aaccataaaa acatctccct tttaattaag
    43981 atattattta aaaataacaa acttttttta ttagaagaga ggaagggatt ttaacccata
    44041 cactctttat ataattgcat cttttaaaga tttagctatt tttaattttg gaactctttt
    44101 ttcctcagtt ttccaaggtt ttttagttcc atctttaaga gttatttctc cttctttacc
    44161 tttttgtatt ttagttaggt atttaactcc catcaacgtc aattcttcac catcttgtag
    44221 tacttcgttt cttttttttt ctatcacatc aagtatctct cttactctta tctgtgacac
    44281 acctaattct tctgctactt cttttaaaaa atctactttt ttcatatctt cttaatctcc
    44341 cttttcttat ataattttca tttgataata gtaaggaata attttattat ttttattaac
    44401 cccttacata agtaatatgt tttaagcgac ccttttttaa accacttttc cccaccagct
    44461 aattacagtt tttacaattt gagcgatatc cctttttacc atttttatca aacctagata
    44521 gcaacttaat ttttccacat ctgctacatt ttttatattt tcctttacaa acagatatat
    44581 aataataatt ttcttcatag tttttctcat atgcatctat tattttgttc actattttat
    44641 ttatttgatt atttattgct tgaggagtta tattcattaa atttgaaata aactctaatg
    44701 atttatcttt tctatataaa tttaaaatat ctttttgtac tgtggtaaat tcaattttag
    44761 atataatatt atttaaatcc ataactatac atgttaaatc atcttgcaaa tcattaccct
    44821 tttgaatctt taaaagctct ttaacatgtg atttatcaaa catatctaaa tagtcccatt
    44881 ctgggcttcc tgcatctgct aatggtgctt tccatataat tggtctctct atagaatttt
    44941 taacatcagt catatcattt tttaatgcat ttaaattttt cctaaccatt ctcttaagtt
    45001 ttaactcttc tttacttaat tgtttatgtc ctaattcttt atatttagct ttataccatt
    45061 cataagtatc tgagtatgtt tttataattg gatatttttt aatatcttta ggtttaactt
    45121 ttatatcttt tattttttta tagtttttag gtaattgaaa aaatgcaaag gaattgtctt
    45181 ctaggaatcc atctttatta gaagtgtttg aatttatatt tccaccattt gcaggtgcta
    45241 ttttatttag aagtgcttct tcctgacatg ctctattaaa taactctttt gaattgtata
    45301 ttcttatttt agtttcctta ggtttatcat ctactgagag tatatagctc cccattattt
    45361 ctaatgtttt tgctatatta gaagtagaat atatgccgtc atctttattt aatatcaact
    45421 taattttcga tgtattatga tgtgtgtact cccatgtacc attactatta ctaaaaggat
    45481 ttttaacatt tatatattcg attggattaa tttgattata gccacaccaa gtaatgaatc
    45541 tattataatc cataactact tcttctccat caatagtaac ataatataca ccatctttat
    45601 ctaaaggact atctgatgtt tgctcaaata tattttccca aaatttatca ttgaattgtt
    45661 ttttttcttg atatttcact ctagtaataa tattctcatt tctctttgta agagtattat
    45721 aaaatgtatc tatataattt ctatttgact cttcaaacca cgtgtgtttt acccctaatc
    45781 gttcctctat gtatttaatt ctatctttgc tattgtctat gctataatct aaattcttta
    45841 tgtaatcttg tgcagatttg ccatctattt cttcataaaa attgttatta ttatatatca
    45901 atattatacc tcctttcctt gcaatccatc atcataatct gtatcatcat aatcccataa
    45961 ttcatcctta aaatcctcct tatgcctttc atttacaata tcatatgaag accttttact
    46021 catctctgtg tatgtatcta tcatttgttc atattcctca tttaatattt gatacctatt
    46081 tttatgtacg cctgttttct ctgattcatc taaatcttct aaatatatta attctatttt
    46141 tttctctttc atacttctcc ttttctatat tataagattt taaattaatt ttcttggtca
    46201 catttctgtg ccgttttttt gccttttggt cacatttctg tgccacccct aaatttaatt
    46261 ttttaactgt ttttcctata tttttgttaa tttttacagt tcattacctc ttattcttag
    46321 acagactttc taaatacaat taaaaaagca taaatcaatt gttttctttg ctaaatttaa
    46381 attatttccc ttccatatta cacaaggatt tataatgaaa tagtctgtat ttttgttatt
    46441 cccttcaacg attacatatt taaaaaaata atatttatca tcttctacct ttatataaaa
    46501 tctaagtaat tcctttttaa atttgttcat atcttgtttt attgtactaa tatttaataa
    46561 ttgacaaatt ttatataaac taattttttt tatttttgaa atatctgttt catgagggtt
    46621 taaacacaag atattactct cgaaatgtat atatggtatg agttgatata tataacttaa
    46681 ttgtttatgt tgtcttggtg tgcaatgttc aaaaagaaat ctactagtat ttatatatat
    46741 tcttgtatat tctttatttt caaaattagg aattccttta gtaaaatact ttggatttat
    46801 ataaaattta ttattagatt catataataa ctcacatttt tttacatctg ctaaaaaatt
    46861 tctaaatgcc ctatctttaa gatttaatat ttctctcatg ttttttctat ctataggaat
    46921 catttcttta aattgagaat atctaaccaa taaattttct tctcctgtat tatagtttag
    46981 atatgttgcg agatatatta acctacttat attagcttta cttatattta cttcattaaa
    47041 taataattca tttttaacat aagtacaaaa tacaaaacct cctaatttaa aattatggta
    47101 tggtaattct tttttattat tgataatagc cctttgctct ggagaaaaag tcttttttct
    47161 ctttccaatt tcaagtacct cacctctttt cattccttta tgttctaata tttctcctgt
    47221 tcttttgtct gttacaataa catcttttat aaattcatta cctctaacta taaatatcta
    47281 cctcttttcc tataaatttt atttcaatta caagataaaa accatcttgc aattgttctt
    47341 cgcaacatgt tgcttcagaa atttcattac cctctatgtt ctatttctat tatatcatat
    47401 accttctatg ttctcaatat tttttttaat atctacccaa aatttcttga gtagatattt
    47461 tcttttaatc tggcatatta ttaaatatat tcttaataat aggattttct ttttttatat
    47521 cttttttttc ttcagtaaca cttgtctttt tatttgtcct atatacacca ttgcctaaac
    47581 attgttttaa taatttattt tcttcttgaa gatttttcca ctctaatatt attctttcca
    47641 aagctgtatt tctactgctt atatttttat cattttgata cttttctatt tcaccaaata
    47701 tatcttcttc taaataaaag ctagatgatt ttttcatata atatcactcc tagaattgtt
    47761 ccatacctac ttcaaataat cctttagccg taaccatttg agcatcttta gagactttga
    47821 atctattatc aaatgtcata tgtaacgcag tacctccact tacatataca tccatttcat
    47881 ctatattttt ccacttagaa gctattcttt gagctgtact ttcagacgcc ataatatagg
    47941 cagccttttt aagttcatca taatcactag aactatctat ttcattaagc tctcttatta
    48001 tacctctatc tttaagttgt ttttgaacta ttgttaaagc atcttgattt ccagcttcca
    48061 atgtatctga tagtttgtca ttaaatacaa aacctttttc aaagtaggat aattctgtgg
    48121 ttctaaatcc tatatgagcc acaccaactg ccttatcttc attcacttca ccttctaaca
    48181 aatgatataa agcagcatcc ccttctctaa atatggatat gtcagatatt tttatttctt
    48241 tatacccacc ctttatttta tcttttattt tgaatgtctt acctttatat gcctctagaa
    48301 cctctttaag tgtagctttt ctaaatgatt tatatggaac acccaacatt atttttactt
    48361 catcagctac agttatttca ttaagagcag cagcaactaa tattctcatt gtcaaagata
    48421 ctttagagtc atcagaattt cttataggtg ttatactttc tttttccgct aataagccaa
    48481 taaaataact tacaccttca aaatctatgt atattggatt ttcatatttc tcaaaatcta
    48541 tgtttcttcc atcaccaaat actgatttga ataaacattc tttaaccatt ccattgtact
    48601 tactatatcc ttttacgtat cctcttccta tatctaagcc tataacttgg taattttctt
    48661 ccattttcat tcctcctaag taaatatttg ctaaatatat tataccatat aaaaacttga
    48721 taattacatt aaatttgcac aaaaattcaa ctataaaatg acatttttta gttattagtt
    48781 aaaattagca ctaaattaga actatatata atttaaattt aaaccaataa tttattatta
    48841 aaaattttta gttaatattt aaattatata tcaactattt ccaaaccaat aaatcaattt
    48901 ctttaactat actatttatg tttttttaga tgtcaatttt aattttaagt aaaatattga
    48961 aacttatttt cttacagacg acattttcat tctaatttaa taaatcttcc tatatttcaa
    49021 gttatttttt gtatttaata tttttatatg tattagttat ttttatgtaa tcaatacata
    49081 cacttttatt ttatattttc cttgttgaga atgtaattta agagtaaaaa tctaagaaat
    49141 atctttagat aaatatttgt tttatgagga aaatacagag ctatttcgtc tgtaaatccg
    49201 tctgcaattt ttttgaaatg tggaaatgaa aggactacac acaaaatcct actgacaaac
    49261 aagccacttt ccataaaata ccccctactt catttttatt tattttaata tgtaaaatac
    49321 tctatttact ctatttaatt aataaaagta ttacttttat cttattttaa aaatatatat
    49381 ttttgtcagt aaaaaaggac ataaaaataa gtattttatg ccatttttcc aaaacctagt
    49441 attttcaata cttataacaa ctacagtttc tattttgtca gtaaaatgtc agtcattata
    49501 attcttagac tacaaacctg ttgtaatcac tgcatttcaa tttttagttg ttgatatcta
    49561 tataaaatat tattttgcta tagaacaaat ttttttatta tggattacaa agtcttatat
    49621 acatcatttt agcattaatc tgacatcgtc gaatatcaac ccacaacctt attgccatat
    49681 ttttcttgac aaatttgtca ttattatata taatttaatt aagataagaa ttaatcttat
    49741 ctatacaatt caatattagg gggtgacgtt atgagaataa caaaaggcaa aataaaagag
    49801 ctaatcgagt tgtgccacca acttgaaaag ctctttgtac aagtatttaa atcacttgaa
    49861 catgttttga tttctttatg ttcttgtatc ggttggttat taatacttat atacataatt
    49921 aaacattaat aaccaatgca atatacctat aaaaatatat ctctataggt atattgtaac
    49981 acacctaata ttgaaagtaa acgaggtgat gacatgaatc aacatattaa aaacacttta
    50041 aaaataataa agatactagg tttattattc ttcttatatt ttgcacttag agcaatatat
    50101 attgtttatc tttaatatct ttattgagta gcttaatgta taagtaactc ttttttgtta
    50161 taatattaat gaggtgatag catgaaatta gaaaaattaa ttgatataag aaaagaaaaa
    50221 aaatattctc aagaggatat tgctagaatg ttagacatat ctttaagaaa ttatcacaga
    50281 aaagaaaaag gagagaatca atttacagta agtgaattag aaaaaatatg tagctattta
    50341 gatatagaat caaaagaact acttgacata tagtaatata atctactcaa ggtagtactt
    50401 aacattaaat aaaataattt aataatataa aatagactag attatagcta gtctatttta
    50461 ttataataac atattgtaca agccatttaa atccattcta agcacatttc aatgcattca
    50521 ccttatgttt atgcctttaa tctttaaaat taaatataaa aataagctag agtatatatg
    50581 tctagcttac agtagttttt ttaatgtgtt gacttgttct tgtactattg ttttactctt
    50641 agtgtctaac tctctataat tatttaatag ttgtttttct tcttcttcta gttgtatatt
    50701 ttctctattg tttgtaagac ataatatata atctatactt acattatata tattagataa
    50761 ttttataagc gtctcattgt caatttttct tctgtttgtt tcatagtttt gtattgttac
    50821 ataatgtacg cctaatagtt ctcccacttg ttgagttgaa tatccttttt cttttcttag
    50881 ctctctcaat ctattcatta aatcacctct taattctaaa tataacatat tataaattta
    50941 attacaattg tattcaaaac ggtatgtttt atatatgata ttatttaaaa cattttgttt
    51001 tgttttttta tattttatat tgacaaccaa aacattttgt tttataatta aatcatagca
    51061 agggagaaca agaaaacact aaactacctc tcaatgaagt aacaggtaaa gaaccttctt
    51121 aaaactctag ctaataaata atcatactag ttggtgaggt gtttccaaca ctaagtaaat
    51181 aagtaaatag aattaataaa ggtttccaat taaagcctgt aaaaaattga ggtatcaact
    51241 tagttgcttc tataatggca ctcaatacaa tttaatatga catagcagtt gtgaggtact
    51301 gcgttataaa aagtactaaa gattgagcat taaaattata gtttgtactg cttgacacct
    51361 acatggttat aaactatcta atatttacta gtataattta aactatttat tagattattt
    51421 tagtaagtgt tagaatacaa cacttaaatt atgatataat aataaaatag gagtgtgaac
    51481 ggcatggatg aaaaaatatt agaattatta caaaaaatgg atactagtat taatgatata
    51541 caaggagaaa taaaatggat taatactaga ttagatgtca tggaaagcga cattaaggac
    51601 ttaaagactg gacaagttga aattaaagac gacttaaaag aaataaaagg tgatatatta
    51661 aaattagatt taaaaacaga ggatacaaga agaaatataa aacttatgca attagacatt
    51721 aataaaatag aaactattac aaattataat ctgaatgaca ttgcaacact taaagcaatt
    51781 aaataggagt gtgaacagta tggatgaaaa aatattagag ttattacaaa aaatggatac
    51841 tagatttgac agaatcgaaa atagactaga tagtatggat ggtcgttttg atggaattga
    51901 aaaccaactt agtgatttaa aacaaggaca agaagaaatt aaaaagaaac tagacttaac
    51961 ttataatcaa gttgctagaa atatggaagg tataacagaa gttggcgaaa aaataaacac
    52021 tttaaaaaat gacatgaact ttgtagaaat ggctacatct aaaaattgga atgaaatagc
    52081 aaagttaaaa tcaattaaat aggagtagat aaaatggatg agaaaacatt agaattgcta
    52141 cgagaaatac aagaaagtat taaaatttta aatgacaagc ttgacgaatt tgattataca
    52201 caagataaaa taaaatctaa tgttgaggga cttttagaat gttttagtcg tattgatttt
    52261 agaattgaag acctagaaaa tggtcaaacc tcattgtata ctaagcttga tatagtgcaa
    52321 aatgaaacag caaaatcaat taaatagcag tacataaaaa gacacttgag tattgagtgt
    52381 cttttctaca agctcttagt atgatattat taaacaataa tatcattgga ggtataacga
    52441 atgtatgagc agagaaaagc aaaagtaata ttcaataaaa gcggggggaa ctctagtgaa
    52501 aattcttaca caaatagggt tacaattcca actatgtgga taaaatatct ggacataact
    52561 aaatcagaca gagaagtctt attgactttt gatggaaata aaataataat agaaaaaata
    52621 gaggagtaga ttaaaaatct actcaaaaaa tacttgacta ttcgtaacga ggggtttata
    52681 attaaaatat caaaataaac aaaaattttt agttaaatat tcgtaacgag agcaaaagag
    52741 agatataata gtacattgaa aactaaatat gacatagcag ttgtgaggta ctgcgttatc
    52801 aaaaacacat caagtattta ttagagtaat ctattgattg ttttaataag tacttgaata
    52861 tagtacttac attcatgtta taataataaa aggagtgtgg tcgtgatgga tgaaaaaata
    52921 ttagaactat tacagaaaat gaacaataat attactgaaa tgaaacagga catcaatgat
    52981 ttaaaaattg gacaggttga aactaacaac agacttgata acatggacaa gcgatttgat
    53041 agtatagaaa atagacttga cagtatggaa aaaaaacaag acattttata taatcaagct
    53101 attgcaacgg ctgaaaatat aacagaaata agagaagatt taggagatgt acaagtcaca
    53161 acgacatgta atctaaatga cttaaaagaa ttaaaaaaac ttaaatcagt taaataagca
    53221 gtgcataaaa agacacttga gttattgagt gtcttttcta taactcttta atgtgatatt
    53281 attattctat aataatattg ttggagggaa taaaatgaat ctacaaaggt taaaagagtt
    53341 aagactagaa catggctata cacaacaaca actagctact catatggata tgagccaaac
    53401 aacttatagt gataaagaaa aaggtaaaac gccttttacg ataccagagt ttgaaaaact
    53461 tcataaattt ttaggtgaag aagtattgga attactagaa ttacaataaa ttctaaaaaa
    53521 atattaacat ttaaccgaaa tattggttgt aaattttcgt taaacatgtt attatagaaa
    53581 tatgaaatag aaataaataa aatcttttac cgaaattctg gttattaaat ttcagtaaat
    53641 taaataaaaa ggaggactat aaaaatgaaa aatctttacc attattttaa cttagaagct
    53701 tttgcaagag atttagaacc ttacaaaagc aggataattg atgactatat agatggagat
    53761 gagatagaat actataacaa tatggatact attgaatttg ctaaactttt cattggagaa
    53821 tcagaaaata ttaaatattt agctaaagaa gtacttgaaa gatattttga ctatgacaag
    53881 tatagaaaag atttaaattc tactaatact atcatcaaat aactaaataa aaagttttag
    53941 ggcgttcttt aaaaacgcca cttccaataa aaaaattgaa aggggaataa atcatgaaaa
    54001 aattaataat taagataaaa tcattgttta aaaaggattt agatagtagc tattggagta
    54061 ctacagaaag tattatttca aaatatggaa acagcaatca attagaatta ttggaacaag
    54121 ctaataaata aggaggtaat aacatgataa attattctga atatatgact gcaacagatt
    54181 taataaatat atgtgacctt ttagaaggtg aaataatagg agaagcagga acttataatt
    54241 ctttatatgt cagactagga aatttaatcg gattagattg ggctttaaag ggagaagaac
    54301 tgagacagca attaatacaa aatattaata atatgatgta tttagataat gaaaatagaa
    54361 ttcaatataa agaattttaa ataatttaag taaaaaaaat tcaactttta agttgatata
    54421 aaatattagg aggatataaa aatgaaaaaa atattattat ttgaaggtgc tggaatgaat
    54481 atatatgaag agggttcaga tgttgaaaat tatagaatta gaactgcttt tataaatggt
    54541 aatggagata aaatatattt agagctaggc ggaaatttac aaaattgcaa aagtgtaaaa
    54601 agattcgggt ggtatacaca tattgaccat tgtttcaata ttggtgtatc gactgatgaa
    54661 aatgtttcaa gaataaaatt gaataataca ttttttaagg gatattcaaa aagagactta
    54721 acaaattata taaatgaaaa tcttaattgt aactttgata ctatagaggt tttagactgg
    54781 atggaaggat acagagtgca tgggaataga aaagaataca atttgatgga tgaccataag
    54841 ttaaatagaa aaagaactat agcaagaaat aaagcatatg aaaaaataga taaagaaata
    54901 atggaaaaat taggagaaaa atattctaaa ataagtcttt attctatgga cgaggactct
    54961 ataactgtta gatgtcatac atatgaaaag gatttatata aaataggcat gagtgaagat
    55021 aatagattat taaaaataca ggttaattat tagattaaaa aaaagaaatt tgaattgaga
    55081 ataaaaatat ttaaaatatt aattttaata tagttggagg ggctaaaatg ttaataaata
    55141 aggctaaagg tattaaaaca tcacttgaaa caagcagctt aagagaacta aaaggataca
    55201 taaaagattt aagaaaagca ctatcaggat ataaatataa gaataaaata gatgaaatta
    55261 cagaatatca aatagataaa ttgtgttaca aaattgataa tacagatata tttgacttga
    55321 aatataaaga aatagaaaag tatatagata acttgatttt gataattaat tttatcttta
    55381 tggaaatgta attaaaagaa tgattttaat ataactggag gtttaagcat gaatgagtat
    55441 attttgataa gttatacaaa aggcgaagga acttgtgacc actgtggaag aactataaga
    55501 aatatagcta caataaaaaa taatgttaca ggagaagttt ttcatgttgg attaacatgt
    55561 gtagaaaaaa taatgaaatt gaatgttaca ttttacaaag cattgtcgag agagataaaa
    55621 aaacattaca aatgtattga gtactacagt aagggtttag atatagagac aaatcttaat
    55681 aaaatagtga agaataatac taaatacaaa gaaggttctt atgcttataa atctaatgaa
    55741 caaatgttag aagatgcaat tgcggaagtg gcttggtctt tggctcgtat gatagatagt
    55801 tgtatgagaa tgaataaact tagtaagtct ggattgatag atatagatat attgaacagt
    55861 ttgtttatca agtataagga atatatgagt aaatttgatg aaaattacaa taaaaacaaa
    55921 tataaatata aaagttgtta tactatgaaa cctttaaaag taatattaga tgaaaatgaa
    55981 gatttaaaag aactatataa aagtatgcaa taaatgtatg taggcgatga attaatgaca
    56041 atgaatttta tatagatgag gattatattt tatataaaag agtgtcatat ctttaatata
    56101 ggacatgaag taataaaaga gtttaaaatt atatttttaa tatgatattc aaattatcaa
    56161 atcgctatta ttataggtat attactatag attaattgat aatgatattt atttgataaa
    56221 aagattttaa agagtataaa aatttaaagg tataaatacc ttaagaggat ttttaaatgg
    56281 cttacaatgg cttgtacaag gtcataatta taaactagga gggtgcaaag atgaaaaatt
    56341 taaaaagtat ggttatagga atgttgataa gtttaattat tgtgggtgtt gcaggaataa
    56401 cagaagttaa ggctgccgaa ttagataatt tttatgtaga gtctacagac agagttgtag
    56461 aaatattaga agatgaaagt gtagttttat atgatactaa agagcaagtt tataatttct
    56521 atcctaaatg tttaggtgat tggaattata gtttttataa taaaaaagat ttagatagag
    56581 cagttgcaac ttacaaagag ctatcaaaca atgtaagtca ctcaaaggat gtatatgtga
    56641 ttaataagtt aaataacaat gaaaacatta aagtattttt aagtgatgga agttcaatta
    56701 tttatataaa aaatgataat aaatattatt tttatccagc ctgtatgggc gactggtact
    56761 taacattaga tagtgaaatt aatttaagtg attgtgttaa cacttatgta gaacattctt
    56821 atatattatt ggatggaggt gttgtaagtg aataataatg ataaattatc tatattaaat
    56881 caattagaat acatagaaaa ggatatcaat gcaaggaatg aaatgattga gaagtttaaa
    56941 gatttattag ttaaatatcc aaaaagtaga gacataagaa agtatataaa acaccaaact
    57001 caatttctaa atggtattaa atatgtatat gataatttag atattttgaa aaatatgata
    57061 aataatgaca agaagtgaga acattactag acataacaat gtctactgaa tataaaagag
    57121 attcatttag taaatacaaa gttattatgt caatttttag ttaaattgta atttaacata
    57181 aaaatgtagt tactatttta aaactcataa taactacatt taataattta aaaagggaga
    57241 tggaaaaagt gaatgaatta aaaatcaaag gaaaacaaaa atttatgaat ttagaaatac
    57301 cagttataga aggtggtttt ggtgaagata aaaaatgtat gacggataag actatatcag
    57361 aaatacataa tcagcctaat tttaagataa gagagagaat aaaggataat ataaaaagat
    57421 ttaaagaaga catagattat atagatttaa aagttatccg tgacacggat gacaatttag
    57481 agctattaca aacacttgga tatagtaaaa tgcaaatatc aaaagctgat aatatatttt
    57541 tattatctga aagaggatat tcaaaattaa ttaagataat ggatactgat ttagcttggg
    57601 agatacacaa taaaataatg gatgaatatt ttagtatgag aaaaaagtta aatgaccctt
    57661 ttgaaaactt atctccagag ttaaaagtta taatcaaact agaacaaaga caagcagaac
    57721 ttgaatctgg tcaacatgag ttagaggaaa aagttaatgt cctatatgat ttttctacta
    57781 tagactacaa tcaacaagaa actattagaa gtttggtaaa tagaagggta atacaagtat
    57841 atcaatgttt aaatttttca gcttatgaag atgctaaatt aagaaaaaga gtatactttt
    57901 cactatggaa agattataga aaaaaatttg gtgttaatag ctataagaac actgctaaaa
    57961 agaactatga caatgcaatt aaattcatag aaaactggga gcctaattat gatttaagca
    58021 atgaaatata catgtttcaa aaagttgaaa atatataaga ataatgattg gagattatta
    58081 tgatatgtta tgtcaaagat atacatacta taagcctata tgatgctttt agatggcata
    58141 caatggggtt gtcattcatt attaaagatg gtaaattaaa aggttttaca atgtaactaa
    58201 ttaattgaga agagaattat gataacattc ttttctcaat taattatcat tcctaataaa
    58261 taaagttcaa aagatagtgt aaaagataat acattttgtt aatattagta atataaaata
    58321 aatttaaaaa tggagatgaa tatattgatt gaaaataagt ttatagaaaa attaaaatat
    58381 accaaaaaca taccaaactc tttatacaaa gacttaagac ataatttttt atttcattca
    58441 aataaaatag aaggaagtac attcacaagt gaaagtttac aagatttaat tgagcacaac
    58501 attgtaaaag gtgaccatac tttagatgat gttataacaa cagtaaattc aagctatgta
    58561 tttgaaactg taatagatac attgggcgaa tacttaactg aaaagtacat aaaggattta
    58621 catggacaac ttatgtataa ttcaaatatt cataatatgg ggctatctgg tatatacaaa
    58681 aaaataccaa atagaataat aggaactaat agtgtattag cccaaccatt tgaagtagaa
    58741 ccaaagatgt tagaacttat tgagtggtat aataatttaa aagatattac attaaaagac
    58801 ttagcagaat ttcattatag atttgaaatc attcatcctt ttcaagatgg caatggaaga
    58861 ataggaagat ttatattact taaacaaata cttgagaata atttgaaatt gattataata
    58921 tcgagtgata ctgtagatga ttatagacag gctttaaaag gttgtcataa tgaatattat
    58981 aaacctttag agcaatattt tgaaagttta gatgactaca aagaaaaaaa taaagattta
    59041 tggagttttt aacctaataa ttaaacaaat gaatggaagt gaaaactatg ctttacagaa
    59101 cggaagattt aaatactttt gcaggtattg gaagttctaa aggtgtttat tgtcaactgc
    59161 cactacccta aagggtagta gcttgtaaaa gctagttgaa tagcctaagt ttacactacg
    59221 ttatgtaaga atatatagct actttagaat actttaaact taaaggaata ttccacatat
    59281 taaaatactc tttttacaaa tttataatta aatcataatc taacttttta tttgaaaaac
    59341 tttcaattaa ccttgcttta tcattagcat tctttaaaat gtagttattt aaccaataaa
    59401 ctatcgcatc acccctataa tggagtccaa tttggactcc attttcataa caccaaaaag
    59461 gatgctcatc ttctgtattg tcaatccaaa tatgaaattc gtttctaatt tcatattttt
    59521 ttaaactaat ttcattagga acatatatat aatcaggact atagttgcca ccaaaattta
    59581 cttttataac cataattata gtctcctttt taaataatta taacatattt tataaaaaac
    59641 tggtaattac acttgtagtt taattccaaa atatagcatt ctacaagtgt atctaaattt
    59701 attaaagcaa aaatataaaa aaataaagga tactatatat ttaaatgcaa taatttttgt
    59761 atttaaatat aaaaactcat atatttatct ataatattat atctattaaa ttataaataa
    59821 ctaaagccaa tatacataag aataaccacc caataaagct ttcattctta ctttcaaaca
    59881 tatttaaaaa ttttaatgca tctttaaata tcaaaataga tagttttata attacaaaaa
    59941 ctgctaatag aataaacaat gttttaagca tatatttctc cttaataaat aatttaaaat
    60001 cttatttaat ataaattttg tttaaattaa tctgttattt ataaataaat tatatcaaag
    60061 tttgttgcac tttagaaata tatgtaaata aaaactatta gtcaactggt taatatggta
    60121 tgacaacaat aatctcatgt agtatactta atttagaggt gagtatatga cttggaatga
    60181 tttaaaaaac aatgatataa catatatagt tgaatatatt aataataagt taaataatag
    60241 taagagtttg actaaagttg ctatagggct tggtgtgtct gaaagtagca taagaaaata
    60301 ccttactaaa agaggttata aacgtataaa tgatgagtat gtgtttattg gtgacaatag
    60361 tatgacaaaa gtagtaaaca atagacaaca gagtaataat gataatacat ttaatatgac
    60421 aatagaagat gacaacaatc acaacatagt aattgacaac caatttaaaa ataatatagt
    60481 ctctttagca aaagactatg ataaaataca agatgtatta aattggtttg agaataaaga
    60541 agatacaaat gtaatagaag ttgtacagga tggtattaag attgatttac attcaaaaga
    60601 tgctataagg actacagtta ggttgaataa agatgcatgg aatttatttg atgagttttg
    60661 tggaaaattc agagagttta ataagtctga tttaatgagt atggctttat tagaatacat
    60721 aaagaagtat gataaataaa aatgacaata aaatcgtagt tttaatttaa ataacctcat
    60781 tattttgagg ttattttttt tattttctat ttaaaacgga gaaggtaaat gttataatat
    60841 atatataatt aaaaaaagga tgtttgaatt tatgaattat aatttaaaaa atgaaaatgt
    60901 attatcagag ctattacaaa atgaggatga aaaaacaagg attggtatac tcaaagttta
    60961 taaagtgtta atattttggt ttgctaaaga aggaaagctt gttgaggaag aaaatgacta
    61021 tattgttgag atagtaggta atgataaaga agaaattgtg agagatatga gccagaataa
    61081 aacatacaat gccataatgg ctataatttt taaaattaat aagatattta aaactttaaa
    61141 ttacgatatt cacttttcta aaccaaaaaa tttagtcgct gaatttgatt tatcaaaata
    61201 tctaagacaa gatgaagtac ttcaggtatg tagtaagcta actaatgcac aagataagtt
    61261 tataatttat gcagcttttt gtggtcttat tggtaaagat gctgaagacc taaggggtat
    61321 aaaaataaaa gacatagatt tagaaaaaag attaataaag ttaaaggata cagaatttaa
    61381 gctggacaag ttcttatttg attttacaaa agatacatta gaacaaaaag agtataaaca
    61441 atatttggag caagacagca atgctgctga gttttatcta tttaattcta attctgaata
    61501 tttaattaaa actaaaccag atgctagata tggaaatgga ttaaaagaga tgggatactc
    61561 aacaattcgc aaaagattac ttattattaa tgattattta gataataaag tacagttgaa
    61621 tttcactaat ttaaaaattt ctggtattat gcatcaaatg catctaaatg aaaaaaaata
    61681 tactcaatct ggatattgga ctaataaaag cttgaaagag tttaaggagg ataacaattt
    61741 aaacttcaaa atacaagaag ttctatctat ttataagcaa aaatatatta aggagtaacc
    61801 ttatgagtaa agatgttaaa gaaaacataa gtaatgaaca aaaagattta aataaaataa
    61861 atttaatcta tcatattata tatgacaatg atagatttaa aatgataaca agaaaatatt
    61921 ttgaaatttt tagattaaag ggtatgtctg ctgaagaaat aagtttaaaa tttgaagaat
    61981 ttctatattc tcttatttca gataagttta aaaagataaa tacagtaaat gatgttaaaa
    62041 aattaagaaa tcaagagaac tcatcatcat taaaacttaa gaagagatgg tctgatatga
    62101 ctttagattc tctaagtgaa gatactaaat cttcaagaaa aaaaagcagt aaatacctaa
    62161 aaaaagaatt agaaattgtt gaaagtgaat atttaaaaaa tggaagaatc attagtgctg
    62221 gagtaaaaaa tccaaaagaa ttaaaggagc aaagaaaaga ttttttagat gagtggaagt
    62281 caagctctag ttataacata tgtgattacc catatttaca agagttaagt actagcaaaa
    62341 taaattccgc atttatacat gatatactaa tgtgtataac agaagtacta aaagatgatt
    62401 atgattttga tattaataaa attgcaatca aaactccacc acatatttca cctggaattt
    62461 ttttaccttt cacaaaaggt aaaagatact cgcacttgaa tgttaaaaaa aatgaaaatg
    62521 aatacatgtc agacgactat agtgctaaga atctttcagg taaacaaatg aattttttct
    62581 ataagttttc aattgggcaa gatgaaaata ttgaaaatct taaactagag ctaataaatc
    62641 ctgatacaga aaaaagggct ttgccaaaaa tatatttaga taaacaagac cttgaaatta
    62701 ttagatttgt ttatacttat agccacatga actctttttc attttattta ggcgatttag
    62761 taaagtactt aggactttca gatggaaaaa agaactatat taatatcaaa aatagactct
    62821 taaaattacc ttattatact ttttattcga atcaaactaa tgataaaggc gaattaaagt
    62881 atgagattag ttttaatttg ttttctagta caacaatagt aaatgatgaa aacaataata
    62941 atagagagtt ggttaaaatc acaaaatctt ttattgaaga agtcgaaaga gttaatacag
    63001 atataatgta tagaaatgaa cttgaaaaat tacaattaac acagtcaatt aatctagctt
    63061 atttcttaga gggtagaagg tcttttctta tatcttgtgg tgaggatata aaaaataaaa
    63121 gttttagata tgacatagaa gatttaaaat ttgatgttca tcttaataaa agtaaaacag
    63181 taaagcaaaa tatggagtat ttagaactag gttttaatga gataaaagaa aatcaattta
    63241 taattaaaga ctataaaaga ggaaattctt attttgatgt ttatttctat gaagattttg
    63301 aaaaaagaaa acgtcttata aataatacta tactttcttt acctgactac atgatagaaa
    63361 ataaatctta aaaatggcca tatattctcc gtacttaaca aaaaatgtcg aatttatttt
    63421 aaataaactt aataaaaaaa gtaaaatgac cgtatattct ccgtacttaa caaaaaatag
    63481 atttttcaaa aaaactaaat gaccgtatat cttccgtact taacaaaaaa tagacctttt
    63541 tttaattatt tccttgtcga aaaatttttt ttgtaacata ttttttactt ttcttagtaa
    63601 tttcaaatct cacgtcttct gaaatcacta tgtctgagct aaatgaccgt atcagctccg
    63661 tacttaacag aagacgttga aaccactcta tttgagctaa atgaccgtat cagctccgta
    63721 cttaacaaat atagacataa atttattttt tgggtaagat aaatttgagt aaaaggaatc
    63781 atcgccaaaa tggctatatc agctccgtac ttctgaaatt attgattttt caaaggagaa
    63841 aattatgagt cttaaatata aagataaaga atttgtactt gtagaaaaaa agaatataaa
    63901 tgaattagat aactctaata taaaatatat tgacctaaag gacaagcagt attatgtggt
    63961 tacacaaggc aaaaaaccta aaagatttaa cctagaagat attaaaaata taaaaaaaga
    64021 tttagataat ggcataagtt taagaaagtg tgcaacaaaa tggaactgta gcactaggac
    64081 tatacaggat ataaaaaata atatttatta atatacagag gtatttattt actttatacc
    64141 tcattaagat tataataatc atatatagag aaaggaggat tataaaatgc aagaattaaa
    64201 accagttgct agaatatcaa agtcaaaact aaaagatata ttagatgttt ttaaaacttc
    64261 tataagaaaa gtagaaaaaa tagattattc tgcttgtaat tcttgtgcaa taaaaaataa
    64321 tccttctttt gatagtcata aattaaatga attttgttca aattgtccta aattaaaaac
    64381 tatatcagag ttttcttatg ttacagaaaa ggaaacagaa ctaggtacaa tagaaattgt
    64441 aactaatgaa agtaaatgta tggctttaag taaaagtgct ataaaacagt atttagcata
    64501 tcattttata gtatctaata aaaattatgt tagaaaagca gtatccttcc aaaatatagc
    64561 cgatatatgc aatgtttcca ttgttacagc cagacataac cataatgtat tactacgagt
    64621 tggattagtt tattctacag aaataaaaag aggtaaattt gatatagtca tagatgaaga
    64681 gtataaaaac catcttaaaa aagaagatgg tggaaaaggt tacttaacta tgtcattaga
    64741 tactttaaat cacttattaa gttttgaaaa tgtaaatgag ttaaaacttg aaattaaaaa
    64801 attaatttgg gctgatgcta aaggaagtaa gttaggtaaa agaatacgat ttaaaaaaga
    64861 caatcttatt tctatacttc cagaatacat aaagaaatca aacaagcgta cagaagcctt
    64921 tttaagaagt tctaaaactt tatttgatgt aagtaatgac acaatagata caaagcgtta
    64981 tgagacgaaa gaaactatct cagagaaata tacagaacca attagaatac aaatatcgaa
    65041 attctttgat aatactggtt gctctttgag ttctaattat agtaaagtgt tatctcagat
    65101 aaatttagat agatataatt tatttgaaga agatttaaga agtaaattag acatggaaaa
    65161 agatattatt ataaatgact tagttggatt gtcggtgcag tatggagtta ctagagtttt
    65221 agcagcatta gattatatgt ttaataaata tacagttgat gaagaagata taaattataa
    65281 tgaaatacgt aatcctggtg cgtttataag aacattgatt gttcagagca taaataaaaa
    65341 tggaagttta gcaatagcaa tttaatatat ggagtagttc aaaaaaaatg attttggcta
    65401 ctcctattag tgtttgtatt caaattataa aatacttaaa tagagctata aagagtatga
    65461 aatctctatt agctctattt ttttatgctt attttaatga aattatatac acaatagaaa
    65521 gttttttata catattaata atttaatttt ccaataacaa tatataatta ttaaatttat
    65581 taagaaactt atcctaaaaa aataataata catcaacaat atattattat taaaatttat
    65641 attttattgt attcattgta attactttga tatatagttt tttttatttt ctaattattg
    65701 aatttaattt ttgtactaat tgctattaat aatttctgta ttaaactaat gagtaagtta
    65761 taactatgaa acttatctta caaagttaat aaatatatat tgcattaaat aataatataa
    65821 ttacattcaa agcgttatat aaatatatat tatttaatac ttcatatctt atatatgtta
    65881 tcatttttat ttaccttatt tttacaaatt attccataag tatattataa ttatcttgag
    65941 ggaaataatc atatagaggg agaaattaga aaatggatag atttgaagta ttaaaatatc
    66001 taaaatttaa tgtttggcat tcagaatggc cattagagct tctcaaaggg gctataacaa
    66061 aggataatgc tactggtaat ttattattac aattaaaaat gtgtaatata tctgataagg
    66121 agataaagtc tgcatatttt acaatagaat gttttaatga tgcaggagat ttaatagata
    66181 ataatttaaa gtatgtttat caagatataa acattaaacc acatgaatgt cttggtgatg
    66241 atattgctat aaaaataaat gataataaaa caagagcaat aaatattata ttagaacaaa
    66301 tagtattttc agatgataaa gttcataaat atgaaagaga agaaggttta attataccta
    66361 aattagataa aatagaattg ttaaatatag aattattaga agaacttaaa aaagattatg
    66421 aagaatatga ttttcaatat gaaattaagt acatgcctaa tataatagaa aaaaaagcat
    66481 ggagctgttg ttgtggaaaa ataaataaat tagaaaaaga taagtgtgat aattgtaaga
    66541 gaagtaaaat actgcaaata aaaaagataa ataaaaatta tttagaatct atttataaaa
    66601 aaagcctaaa aaagaaaaaa gaaatagaaa taaagcttga aaaagataat agacataaat
    66661 taaagaacat aaataaaaat aatagaagaa catcaatatt attaataagt atattattga
    66721 tttttattat ttttatt
//
LOCUS       DAEORZ010000002        63015 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEORZ010000002 DAEORZ010000000
VERSION     DAEORZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 63015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.002"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            1..1689
                     /locus_tag="KRH39_000073"
                     /pseudo
     CDS             1..1689
                     /locus_tag="KRH39_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
     gene            1701..2768
                     /locus_tag="KRH39_000074"
     CDS             1701..2768
                     /locus_tag="KRH39_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG8510008.1"
                     /translation="MKFRKLVSLGILSTLLLSVITGCNNASSGSNLKSNNKIKGELTV
                     YTAIEEDSIDTYLTTFKEKYPDIKLNVLRLSVGDITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            2960..3490
                     /gene="rnmV"
                     /locus_tag="KRH39_000075"
     CDS             2960..3490
                     /gene="rnmV"
                     /locus_tag="KRH39_000075"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBG8510009.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            3661..4530
                     /gene="rsmA"
                     /locus_tag="KRH39_000076"
     CDS             3661..4530
                     /gene="rsmA"
                     /locus_tag="KRH39_000076"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBG8510010.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEIGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            4596..5858
                     /locus_tag="KRH39_000077"
     CDS             4596..5858
                     /locus_tag="KRH39_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510011.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEDNEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDSEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYRYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            6009..7124
                     /locus_tag="KRH39_000078"
     CDS             6009..7124
                     /locus_tag="KRH39_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG8510012.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            7310..8206
                     /locus_tag="KRH39_000079"
     CDS             7310..8206
                     /locus_tag="KRH39_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG8510013.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            8276..9073
                     /locus_tag="KRH39_000080"
     CDS             8276..9073
                     /locus_tag="KRH39_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510014.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(9129..10481)
                     /locus_tag="KRH39_000081"
     CDS             complement(9129..10481)
                     /locus_tag="KRH39_000081"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBG8510015.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            10700..11578
                     /gene="purR"
                     /locus_tag="KRH39_000082"
     CDS             10700..11578
                     /gene="purR"
                     /locus_tag="KRH39_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBG8510016.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            11734..12012
                     /gene="spoVG"
                     /locus_tag="KRH39_000083"
     CDS             11734..12012
                     /gene="spoVG"
                     /locus_tag="KRH39_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBG8510017.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            12225..13604
                     /gene="glmU"
                     /locus_tag="KRH39_000084"
     CDS             12225..13604
                     /gene="glmU"
                     /locus_tag="KRH39_000084"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBG8510018.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEAEVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            13699..14649
                     /locus_tag="KRH39_000085"
     CDS             13699..14649
                     /locus_tag="KRH39_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBG8510019.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      15490..15575
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            15737..16255
                     /locus_tag="KRH39_000086"
     CDS             15737..16255
                     /locus_tag="KRH39_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG8510020.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKMPATTSVSDLSELKTYMDTLPDKADIGGKYQLLLVDK
                     KLVLQIGDKDGGATLTKAQSAKLLSDLGKNKIYKEADLKDTNELKKDDEINNKNLYIV
                     LIDNADMDSATA"
     gene            16406..18082
                     /locus_tag="KRH39_000087"
     CDS             16406..18082
                     /locus_tag="KRH39_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG8510021.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESITIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            18094..19302
                     /locus_tag="KRH39_000088"
     CDS             18094..19302
                     /locus_tag="KRH39_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG8510022.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVFLVIILFIYQFKKYLKTPVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVSILVVSIF
                     MPMLSIYDAM"
     gene            19317..21029
                     /locus_tag="KRH39_000089"
     CDS             19317..21029
                     /locus_tag="KRH39_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBG8510023.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLILDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQLKAKQMKNILK
                     IVPIVILILAIPYLCLIFSNNLANKELENVNGEIKNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTADK
                     SSNNETKSNTKSDSNGDKTNNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFEDVKLHSIKSNAEDSTYTFSMTLYLKEGV
                     LTNE"
     gene            21022..21930
                     /gene="pilO"
                     /locus_tag="KRH39_000090"
     CDS             21022..21930
                     /gene="pilO"
                     /locus_tag="KRH39_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBG8510024.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVREYEHFYGVSTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            21957..22523
                     /locus_tag="KRH39_000091"
     CDS             21957..22523
                     /locus_tag="KRH39_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG8510025.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            22560..23087
                     /locus_tag="KRH39_000092"
     CDS             22560..23087
                     /locus_tag="KRH39_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG8510026.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            23108..24646
                     /locus_tag="KRH39_000093"
     CDS             23108..24646
                     /locus_tag="KRH39_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510027.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            24742..25824
                     /locus_tag="KRH39_000094"
     CDS             24742..25824
                     /locus_tag="KRH39_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBG8510028.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            25805..26587
                     /locus_tag="KRH39_000095"
     CDS             25805..26587
                     /locus_tag="KRH39_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG8510029.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKVYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            26689..27339
                     /locus_tag="KRH39_000096"
     CDS             26689..27339
                     /locus_tag="KRH39_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG8510030.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            27353..27913
                     /locus_tag="KRH39_000097"
     CDS             27353..27913
                     /locus_tag="KRH39_000097"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBG8510031.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            27930..31316
                     /gene="mfd"
                     /locus_tag="KRH39_000098"
     CDS             27930..31316
                     /gene="mfd"
                     /locus_tag="KRH39_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBG8510032.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQIIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            31347..32342
                     /locus_tag="KRH39_000099"
     CDS             31347..32342
                     /locus_tag="KRH39_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG8510033.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            32466..33005
                     /gene="spoVT"
                     /locus_tag="KRH39_000100"
     CDS             32466..33005
                     /gene="spoVT"
                     /locus_tag="KRH39_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBG8510034.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            33125..34738
                     /locus_tag="KRH39_000101"
     CDS             33125..34738
                     /locus_tag="KRH39_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG8510035.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            34845..36293
                     /gene="mazG"
                     /locus_tag="KRH39_000102"
     CDS             34845..36293
                     /gene="mazG"
                     /locus_tag="KRH39_000102"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBG8510036.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            36382..36660
                     /locus_tag="KRH39_000103"
     CDS             36382..36660
                     /locus_tag="KRH39_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBG8510037.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            36732..36974
                     /locus_tag="KRH39_000104"
     CDS             36732..36974
                     /locus_tag="KRH39_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBG8510038.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            37036..37290
                     /locus_tag="KRH39_000105"
     CDS             37036..37290
                     /locus_tag="KRH39_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBG8510039.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            37291..37824
                     /locus_tag="KRH39_000106"
     CDS             37291..37824
                     /locus_tag="KRH39_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510040.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNEKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            37830..38114
                     /locus_tag="KRH39_000107"
     CDS             37830..38114
                     /locus_tag="KRH39_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBG8510041.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            38227..39147
                     /locus_tag="KRH39_000108"
     CDS             38227..39147
                     /locus_tag="KRH39_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBG8510042.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            39237..41609
                     /gene="spoIIE"
                     /locus_tag="KRH39_000109"
     CDS             39237..41609
                     /gene="spoIIE"
                     /locus_tag="KRH39_000109"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBG8510043.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVESIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            41960..43852
                     /gene="pepF"
                     /locus_tag="KRH39_000110"
     CDS             41960..43852
                     /gene="pepF"
                     /locus_tag="KRH39_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG8510044.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            43966..44931
                     /gene="galU"
                     /locus_tag="KRH39_000111"
     CDS             43966..44931
                     /gene="galU"
                     /locus_tag="KRH39_000111"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG8510045.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            45431..47035
                     /gene="rho"
                     /locus_tag="KRH39_000112"
     CDS             45431..47035
                     /gene="rho"
                     /locus_tag="KRH39_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBG8510046.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            47188..47388
                     /gene="rpmE"
                     /locus_tag="KRH39_000113"
     CDS             47188..47388
                     /gene="rpmE"
                     /locus_tag="KRH39_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBG8510047.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            47533..48426
                     /locus_tag="KRH39_000114"
     CDS             47533..48426
                     /locus_tag="KRH39_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBG8510048.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            48444..49292
                     /gene="prmC"
                     /locus_tag="KRH39_000115"
     CDS             48444..49292
                     /gene="prmC"
                     /locus_tag="KRH39_000115"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBG8510049.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            49336..50400
                     /gene="prfA"
                     /locus_tag="KRH39_000116"
     CDS             49336..50400
                     /gene="prfA"
                     /locus_tag="KRH39_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBG8510050.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            50529..51209
                     /locus_tag="KRH39_000117"
     CDS             50529..51209
                     /locus_tag="KRH39_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBG8510051.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            51238..52278
                     /locus_tag="KRH39_000118"
     CDS             51238..52278
                     /locus_tag="KRH39_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBG8510052.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            52295..52744
                     /locus_tag="KRH39_000119"
     CDS             52295..52744
                     /locus_tag="KRH39_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBG8510053.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            52761..53213
                     /gene="rpiB"
                     /locus_tag="KRH39_000120"
     CDS             52761..53213
                     /gene="rpiB"
                     /locus_tag="KRH39_000120"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG8510054.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            53249..53878
                     /gene="upp"
                     /locus_tag="KRH39_000121"
     CDS             53249..53878
                     /gene="upp"
                     /locus_tag="KRH39_000121"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBG8510055.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            54179..54994
                     /locus_tag="KRH39_000122"
     CDS             54179..54994
                     /locus_tag="KRH39_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBG8510056.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            55220..55657
                     /locus_tag="KRH39_000123"
     CDS             55220..55657
                     /locus_tag="KRH39_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG8510057.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            55842..56069
                     /locus_tag="KRH39_000124"
     CDS             55842..56069
                     /locus_tag="KRH39_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBG8510058.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            56074..56460
                     /locus_tag="KRH39_000125"
     CDS             56074..56460
                     /locus_tag="KRH39_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBG8510059.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            56463..57167
                     /gene="atpB"
                     /locus_tag="KRH39_000126"
     CDS             56463..57167
                     /gene="atpB"
                     /locus_tag="KRH39_000126"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBG8510060.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTKNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            57236..57496
                     /gene="atpE"
                     /locus_tag="KRH39_000127"
     CDS             57236..57496
                     /gene="atpE"
                     /locus_tag="KRH39_000127"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBG8510061.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            57619..58116
                     /gene="atpF"
                     /locus_tag="KRH39_000128"
     CDS             57619..58116
                     /gene="atpF"
                     /locus_tag="KRH39_000128"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBG8510062.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            58113..58658
                     /locus_tag="KRH39_000129"
     CDS             58113..58658
                     /locus_tag="KRH39_000129"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBG8510063.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            58675..60177
                     /locus_tag="KRH39_000130"
     CDS             58675..60177
                     /locus_tag="KRH39_000130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBG8510064.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            60205..61083
                     /gene="atpG"
                     /locus_tag="KRH39_000131"
     CDS             60205..61083
                     /gene="atpG"
                     /locus_tag="KRH39_000131"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBG8510065.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            61096..62490
                     /gene="atpD"
                     /locus_tag="KRH39_000132"
     CDS             61096..62490
                     /gene="atpD"
                     /locus_tag="KRH39_000132"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBG8510066.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            62493..62753
                     /gene="atpC"
                     /locus_tag="KRH39_000133"
     CDS             62493..62753
                     /gene="atpC"
                     /locus_tag="KRH39_000133"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBG8510067.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT        25392 a         6770 c        11634 g        19219 t
ORIGIN      
        1 tgataacatc aaaaaataaa agaagaataa gtgatagcac aatacaaaaa tatttcttga
       61 ttttttcggt aattgtacta atagtattta tattatttcc attagtatca ttattaaaaa
      121 atgcatttat ggatacaagt ggaagttttg taggaatttc aaactttagt aaatatattg
      181 aaaatcccac tcttatagca tcatttaaaa actcaatttt tgtatctaca atttcatcct
      241 taatatcact atgtttagcg tttatatatt catatgcaat aacaagaacg aatataaggt
      301 ttaaaaactt ttttaaaact atgggcatat tttcgctttt cgcaccagct atgctatatg
      361 gtatttcact tatatacctt tttggaaaca agggaatctt tacaattata ggcatagaag
      421 taccactata tggtccatta ggaataatta tttgtcaaat actgtttaca tttccacagg
      481 cctttttaat attatctgta gcactttcga tggctgatta tagactttat gaagcagcag
      541 atagcttagg ggctagtgaa tttaagaagt tcttcaaaat aacaattcct ggtataaagt
      601 attcattagt tagttcgttc tttgtatctt ttatacttgc atttacagat tttggagcag
      661 caaagatagt tggaggaaac tacaatgtat tggctactga catatataaa caagtggtag
      721 gtcaatttaa cataccaatg ggagctactg tctctatggt aatgttaatt cctgtactaa
      781 tagcatttac agtagataag atagcaagta aaaagcaagg gatgactata actgcaaagt
      841 ctatgccata taagattaag gaaaataagt ttagagatag agtattttta atagcatgta
      901 gttttataac tttattaatc tttattttga tatttgtttc agttataggc tcagttataa
      961 aattatggcc atatgattta agctttacaa tggaacacta tacgtttaaa ggtatatcag
     1021 gaaatggaat caaaacatat ttaagcagtt taaaaatatc aattttaaca gcaatatttg
     1081 gaactatatt tgtatttttt agtgcttata tgatagaaaa agtggataag tttcctagac
     1141 tgagacaaat atcttatttt ttatcaatga taccaatggc attacctggt ttagctttag
     1201 gtatatcgta tataactttt ttcaattcag caagtaatcc attaaatttt ttatatggaa
     1261 gtacaacaat aatggttata gccaatatag ttcattttta ttcagtttgc tttataactt
     1321 caaactcatc tttaaaaatg ttggatagag agtatgaatt agttgcaaaa tctataaata
     1381 ttccatttta taaggtgttt ttttatgtaa cacttccaat gtccataaca tcaatacttg
     1441 aagtggctgt atattacttc gtaaattcaa tggttactgt ttcagcatta atatttttat
     1501 atatacctca aactcaaact gcatcaatat cgatactaaa gttagaagag atagggtata
     1561 tagggccatc agctgctatg gcagtattag tgtttttaac aaatataata gtaaaacttt
     1621 tgtatgaatt gacaaccaaa gaaattaaaa actaagacac aaaaatggca acaaaaagaa
     1681 gttgcataga ggaggtaaaa atgaaattta ggaaattagt ctctttagga atattatcaa
     1741 cacttttatt gagtgtaata actggatgta ataatgcttc atcaggttca aatttaaagt
     1801 ctaataataa gataaaagga gagttaacag tttatacagc aattgaagaa gatagtatag
     1861 atacatattt aactacattt aaagagaaat atccagatat taaattgaat gtacttagat
     1921 tatcagtagg agatataaca gcaagattat tagcagaaaa agataatcct aaagcagatg
     1981 tgttatgggg ggttgcagct acaagtttga taattttaga tgacaaagaa atgctagagc
     2041 catacgctcc gaaaggcttt gataaggtaa atcctgtatt taaagatgat aagaaagttc
     2101 cttcttgggt tggaatagat gcttggatga ctggtataac agtaaatact aaagaattaa
     2161 gtgataagaa cataccagtt cctagttctt atgaagattt aataaagcca gaatataaag
     2221 gtcttatatc tatgcctaat ccagcttctt ctggaacagg atatttaaca gtatcagaac
     2281 ttatacaaat tatgggtgaa gaaaaagcat ggcagtatat ggataaattg cataaaaata
     2341 ttggtgtata cactcagtct ggttcagctc cagctatatt agcagcttca ggagaatatc
     2401 ccattggtat atcttttgga aatagaggta taaagttaaa aggtgaaggt tatccagtag
     2461 aagtagtatt tccaaaagaa ggttctggat gggagataga atcaaatgca cttgtgaaaa
     2521 aagataatat aaaagaagca tcaaaagtat ttttagattg ggctataagt aaagatgcaa
     2581 tgaatgagta ttctaagaat tttgcagtta ctacaattga cacgggaaat ccggtgccag
     2641 aaggatttcc aaagaatcca ttagaacaga tgatagataa tgatttaaaa tcagcagcaa
     2701 gaaatagaga ggctatacta aataaatgga tagaaaaata tgatggaaag acagaaaaag
     2761 agagctaagc cttgcaataa aatttagtac aaaaagcgcc tagaatataa gttttatatc
     2821 taggctcttt tgatatattt atttaaaatg catatttcta tataaaaata aatagtttag
     2881 taccttgatt aaattaaata aaaaccttaa aataaagaga tgtaaagaaa aactaaaaac
     2941 gaataaaagg tgaatttata tgataaaaga aataatagta gtagagggta gagatgacgt
     3001 tacagcagtt aagagagcaa tagatgcaga acttataact acaggtggat ttggatttcc
     3061 aaaaggtgta atggagcgta taaaagctgc taatgaaaga cgtggtgtta taatatttac
     3121 agacccagat tttgcaggtg aaaaaataag aaaaaaaata gcatcagaag ttccaggatg
     3181 taaacatgca tttctaccga gagaggaagc taaaaaggat ggagatatag gtattgagaa
     3241 tgctactcct caaagtataa tagcagctct gaataaagtt agaactgaaa atacagaaaa
     3301 aagagatgaa tttaagcaag ttgacttaat tagaaatggt cttataggta atgaagaagc
     3361 atcacttaga agagatgcac ttggaaaaat acttggaatt ggatatggaa atgcaaaaca
     3421 atttttaaat agattgaata attatggagt tgaaagagaa gaatttatag atgcattaaa
     3481 acaaatctaa acgtaatgtt taatgtatga aaaatatgta tataaaataa attgtggaat
     3541 tacggttaat ttaagataaa ttatttataa tatacacctt attttaaatc aatgtaaata
     3601 catataaata agtataatta ataaaattaa atatcaaaat aagttaaaaa ggagtaaata
     3661 atggatagac tatcttcaca taatgcaaca aaggaagtag tacaaaaata taattttaaa
     3721 ttttcaaagt ctttaggtca aaatttcttg atagatagta atataattga taaaatctta
     3781 tctggagcta gaatcacaag aggagataat atcatagaaa taggacctgg tataggaact
     3841 cttactcgtg agatgggtaa aatagcagaa aaagtagtgg ctatagaaat cgatagaaat
     3901 ttaattccta tattgaaaga taccttgtca gatttagata atacagaggt tgtaaatcaa
     3961 gatatactaa aagtagacat acaggaatta gtaaaagata aattaaatgg tgggccagtt
     4021 aagcttgtag caaatcttcc atattatata actactccaa tagttatgaa atttttagag
     4081 gaagacatac ctgtaacaga catagtagtc atggttcaaa aagaagttgc agatagaatg
     4141 aatgctattc cgggtactaa agattatggg gcattatcta tagctgttca atactattgt
     4201 gatacagaga ttgtggcaaa agctccaaga catatgttta taccacaacc aaatgtagat
     4261 tccacagtta taggtttaca tgtaagagat aaaagaaaat atgatgttca taatgaagac
     4321 atatttttta aaacagtaaa agcatccttt ggacaaagaa gaaaaactct tcttaattcg
     4381 ttgggaggtc ttggttttct taataaagat gaaataagag aaatattaaa agaagctaat
     4441 atagacgaaa aaagaagggg tgaaactctt agtatagaag agttttcagt tctttcaaat
     4501 ataataaata caaaagtatc ttctaaatag aaggtacttt ttttaatttt gttaacatat
     4561 attgtttaga gaaactgtgt taagggggga ttgcagtggg ttctaaaaga aagtttaaaa
     4621 gagactatat aatactagaa gctaaagaca taaactttag atacaaagaa cgagtactgc
     4681 caaaagcttt tgcaaaagtt gaagtaaatg atgaaaaagc aattattgca ttatatgtag
     4741 aaaatctaaa atatgttaaa gatgggtata aagcagtagc aataaaagat gaccatggat
     4801 taatagactt aggaaatata attttaaatg aacaaggaag aggtgaattt gttttagatt
     4861 tagaagatta tgatgatgag ataaaaggta ttgcattatt gtatgaaaga aatatacctt
     4921 taattggatt taagggaaat aagcttgaaa attatgagga catattattt caagaggtag
     4981 aggaagaaga atatgaagaa tatgaagagt tggaagaaga agacgaagag atagaggata
     5041 atgaggaaag tgatagagac gaagatagtg aagaagaata tattatagaa gaatatgatg
     5101 aagatgagta tgaagactat tgggaagatg atgaagatag tgaagaagga gagttaaaag
     5161 caagaacgga agtagaagat tctggtgatg aggaaataga agagttcaat aattataagg
     5221 aaatagaaaa ttataatagg gttaaagtac aggaagaata tatagacata gaatatgatg
     5281 aggaagaaga ggaagaagaa gtttttgaag taaatagaga agaagatagg tttgtttcct
     5341 caaaacctac taaatcaaag aaagttcaaa aagatcagaa taatattgaa aaaataaaaa
     5401 gagctaatgg agtaagtcaa ggagagagtg gaaataaaca aaccagtaca ttacttatgc
     5461 caagacaaat aaaaaaaggt ttgaaattat ttaaagaagt aaaacctttt gtaacagatt
     5521 atatagataa gacaagatgg tggaaaatag agataaatcc tacaacactg tgtggatata
     5581 caatgcctta tttaggatat ttaaacgtat tgaattacac tatgtatagt gatgcagttt
     5641 tacattcata tagatacaga cactatttat ttggagttca gtatgatgaa tataataaga
     5701 gaaaacatta tatttatgct atcccaggaa atagaaatga acagccagac aatggaagca
     5761 caggattttt aagatatcaa gcttgtgata atagaacaaa tagtttagga tattggctat
     5821 gttttataga ttctagacaa agaaatatag ttaggtaaaa ataataaaaa tgaaaaaatt
     5881 tagactcaat tcttatttta ctgaaaaaat taatgaaatg tagtacaaaa ctttaaaaac
     5941 tattgataat atttaaatat tacactacaa tatatcttga aatacctatt atattatgga
     6001 ggaaaattat gataaacgag caaagaattt taaatgagtt tcttgaatta gtacagatag
     6061 atagtttatc tttaaaagag gggaatgttg ctaaagtatt agttaaaaaa ttagaagaaa
     6121 taggttgttc agtagtaata gacaatgctg gtgaaaaagc aaatggagaa actggtaata
     6181 taatagctac attaaaagga aataaagaag gaaagaaaat attatttagt tcacatatgg
     6241 atacagtaac tccaggtata ggggtaaaac ctatagtgga tgaagcaaat ggaataataa
     6301 aaagtgatgg tactacaata ttaggttctg atgataaagc aggtatagca gctatattag
     6361 aaggcttaag atatataaat gaaaataaca tagaacatac tgatatacaa gttgtattct
     6421 ctatatgtga ggaatgtgga ttagttggag ctaaaaattt agactatgga aaaatagatt
     6481 ctgaatatgc atttatatta gatagtggtg gttctccagg tgaaataata gtaaaagctc
     6541 ctgcacaaga tgtaataaat gttaagatat taggaaagac tgctcatgca ggtcttgaac
     6601 ctgaagctgg aataagtgct ataatggtag ctgccagagc aatagaaaat atgaatttac
     6661 ttagaataga cgaagaaaca actgctaaca taggaattat aaacggtgga acagctacaa
     6721 atatagtaac aggagaagtt aatataattg cagaagccag aagtctaaaa gaaaataaat
     6781 tagatgttca aactaaacac atggtagaga cttttgaaaa agctgcaaaa gattttggag
     6841 ctcaaattga gatagatgtc aatagagcat atacaccaat agatgttagt gaggactctg
     6901 aaataataaa acttgcaaag aaggcttttt caaacttagg tatagaaggt catactgaat
     6961 ctacaggagg aggtagtgat acaaatatat taagtaagaa tggtatcgaa gctataactt
     7021 taggaattgg tatgaaaaat gctcatacac taagtgaaca tatagctata aaagacttat
     7081 atgattctgc taaaatggta gtggagataa taaaggaagc ataaatttaa ataatagtgt
     7141 ataatatata tatcgcctag cttaagctag gtgatttttc attttataga agattgataa
     7201 tctctattga aagcaaagag tatacatttg catataagag agtgtttaat ttatgttcaa
     7261 atgtaggttc ttaataatat ttagattgtt tcataaggga ggaataaata tggaaacaag
     7321 gtatgaagaa gcaaataaaa taacaataca atcaatactt tggaatgtag tattaacaat
     7381 aattaaggtt atagctggtg ttattggtaa ttctagcgct atgattgcgg atggattaca
     7441 ttcagcatca gatattataa gttctatagg agtactaata ggcaattatg tatcttctag
     7501 acctggagat agagagcata attatggtca tgagaaagca gagacattag tatcatttgt
     7561 gttatcaata ttgcttattt tcgtttctat aacaataggt atagaggcaa ttaaatcttt
     7621 atttaactta gatgcattaa gtgttccatc aatattacca ttagtagtat cagttatttc
     7681 aatcctgata aaagagtatc aatatagaat aacaataaaa gttgctaaaa agataaattc
     7741 accagcccta aaagctgatg cttggcatca taggtcagat gctttatctt cagtagctgc
     7801 ttttataggt ataggtggtt ctattttagg gtttaaacca ttagacccaa ttgcgtcagt
     7861 tgtagtggct atatttgtag ctaaagtagg aattagtata cttataagtt ctgttaatga
     7921 attgatggat gtttcagttg atgaagaaga gataaaagaa ttaaaattta ttgtagcaga
     7981 tacagaaggt gttaaaaatc ttggtgatat aaagactaga aaacatggag ctatggcata
     8041 tgtagatttg actatatgtg tggatgaaaa tttgacagtt aagcaaggtc atgatattgc
     8101 taccaagctt gaaaaacata taataaaaca tatggaattt gtaaaaggca taacagttca
     8161 cgttgaacca tgtactaatt gccaaggaaa taagtgtaat aattaattaa aacttaaaaa
     8221 aaagtgtaaa aagttatata attgttttat gattataaac tctaggggtg gttaaatgag
     8281 caagaaaaga aaactaaaga aaaaagctgc aatattaata ggagtagttg ttttctttat
     8341 aatatacttt atatccttta gaataggaat aaatatggaa aaggataaaa gtgaagtgcc
     8401 aaagaaggta gaaaaagaag aagaagataa taggtcttta aggactcaaa taaaagagaa
     8461 gaagaaggtt tatatatcag ataaaaatat agaaaacata aaaattgaag aagatatgtg
     8521 ggatgaaata aagttatttt tctcagattt taagaaagta agaaatacta cttcttctta
     8581 cgaagcagtt tatgaaggtt attctgatga tggagttaaa ttttctactg accttaactt
     8641 ttttagagta tatactgtaa ataaagaaga atattataaa gtaccagtta gttctaagga
     8701 tgcttttaag aaacttttag atgagagtat atatacttcg tttgaccttt taagtcatta
     8761 taaagattgg aaagaggtta ctataactca tggaaaagaa attaaaaaag ttcatagatg
     8821 gaaatttgat gatttagcac ataagatgat ttcaaagaga gttgtaggaa aagttcaacc
     8881 tgagaaaagt aaggaaagaa gtgactataa ttttactata aatataaaag gtgataattt
     8941 tgatgtaaaa gttgatacaa tgggaaaaga ttatgtaaaa gttacttcta agaaaaatgt
     9001 atcttactat gaggttcata caagtttatt tgattacctt aaaaatgaga tatttgaaat
     9061 tggaaagaaa taagacttta agaaggctat caaattagat atttgatagc ctaaattatt
     9121 tttatatatt atttttcaac caacaagtct gcaaccttat aaattggacc agcaccagca
     9181 gttattacta aatcattatc cttaacattt tcacgtaagt attttactat atcttcaaat
     9241 tcttttatat agatggcatc tacattgttt tgatataatt tctcaaccaa gtccttagaa
     9301 tgtatatctc cagggtcttt ttccctagca gcatatatat ctgttatgat aactttatca
     9361 gcagcataga atgcctctga aaattcacct aaaagagatt ttgttctagt ataagtatgt
     9421 ggctggaaga tacaccataa agttgatttt tttaattttt tggcagcagc taaagttgct
     9481 tttaactcag ttggatgatg tgcataatca tctactacta aagcattctt ataataccct
     9541 ttagtttcaa atcttcttcc gacaccttta taaattttta tattttttct tatagtctct
     9601 aagtcaatac cagaaacaag agctgccatt atagcagaag aagcattgta tatgttatga
     9661 agaccatata cagaaagttc aaattctcct aaatctcttc cttcatattc tattctaaat
     9721 attccatgac cactattgtc aaagtgaata tcttttatta cagcatcatt gtcagaatct
     9781 ctaccgtatt ttataatagt agctttaaca tcatacaata tgtcatcaat attttcatca
     9841 tcgccattta ttatgaaata gccttctggg ggtaataatt ttccaaattt gttaaaagaa
     9901 gccttgattt catctattcc agagaaataa tcaagatggt cttcttcaac atttaaaact
     9961 atagatatct ttgggttaaa attaaggaaa ctatctacat attcacatgc ttcagttata
    10021 aagtggttgg aattacctat cttaacatta ccacctatca tacttaagtt accaccaact
    10081 agtatagttg ggtctaagtc tgcatattca aagattgtag ataacataga tgttgtagaa
    10141 gtttttccat gtgttccaga aactgctata gaatttttat attccctcat gatttggcct
    10201 aaaaaagttg ctctgttcat taccaattta tttttttctt tagcagccac aagttcttca
    10261 ttatctggat gaactgctgc tgtataaaca accatattta catcatctga tatattatct
    10321 tttttctgac caatataaat agttgcccct tggtccctaa gtttgtctaa taggtatgat
    10381 tcgtttgaat ccgaacctga aacttgataa cctttattga tacatatttc agctaatgca
    10441 ctcatgctga taccgccaat tcctataaag tgtatattca taataaaacc acctttcttt
    10501 ttaattaagt cctacaatat gaactatttc tgtggtataa taatataatg ttcaatattt
    10561 tataattaat atgcatataa tattatatgt attttatttt tgaatataat aaaacataag
    10621 taatattata ccacaataag aattgttatg cattagttag tttaacgtta tataaaggta
    10681 cttgtaggag gaaataataa tgaaatttaa aagaacagag agaattggag caatagttaa
    10741 aatactgtct gataatccaa ataaaatata tactttaagt tattttacaa atcaatttaa
    10801 tgcagcaaaa tcaacaataa gtgaagattt attagttgta aaaaatgtat ttgagaagtt
    10861 acatttaggt aaagttataa ctatttcagg agcagcagga ggagtaaaat atatacctaa
    10921 aacttctata gcagaaaatg cagagttctt aatggaatta tgcgagagaa tatgtgacaa
    10981 gtctagaata ctatctggtg gttttttata cttgattgac ctaatttacg accctacaat
    11041 agctgctaag ataggaaaga tattcgcatc aaatattgag tatgtagatg cagattatgt
    11101 tgtgacaatg gaaacaaaag gtatacctat ggcacttatg actgcaaaag ctatgaattt
    11161 acctctagtt ataataagaa aagatataaa ggtatcagaa ggtcctacac ttagcatgac
    11221 atatgtgagt ggaaacagtt ctaaagttga aagtatgagt ttgccaagaa aagctcttaa
    11281 accagatagt aaagttataa ttatagatga tttcatgagg ggtggaggta ctataaaagg
    11341 aatggtagac cttatgaatg aatttggtgc agaggttata ggtactggag tattcatatc
    11401 tacaacgaat ccttcagaaa aaatggtaaa agattatatt tcgttgattc aattggatgt
    11461 taatggagat aaaatagtag tagaaccaaa cttaaagact tttaaagatg aatatagaaa
    11521 tgaagattta gacgaagaag aagattcaga atttgaattt gagatagatg aagattaata
    11581 aaaaaaatat ttagaaaaat atgtaaaaat aaaaagagga tatccctagt tgttcataga
    11641 attatttaaa aaacagttta tattaattta ttccagcgat aataatttta atataaatta
    11701 atttctgtca aaatcaaagg gggatatttt ggtatgaaga taactgacgt aagagtaaga
    11761 aaattaacag aagaaggtaa gatgaaatgt atagtttcaa taactttcga taatttgttt
    11821 gtagtgcatg atataaaagt tatagaaggc cataatgggc tatttatagc aatgcctagc
    11881 agaaaagtag gcgaaggaaa tttcagagat atagctcatc caataaatgc agaaatgaga
    11941 caagttttag aagatgcagt tttacaagcg tatcatgaag cattagttca atgggaagta
    12001 gctgctgaat aataataaat atataaaatg gagagagccc gaacatttag gctcttttta
    12061 aatgtagaat ttctattaaa atatgtactt tttagtaaaa aagtgctata ttattagtga
    12121 taatgtatat gttgtataca aattgttaga taaatgtatt ataatgtata ttgattaaga
    12181 gaataatacg aataaaatta aattagattg aatggagtgt atcaatgaac tttaaagcta
    12241 taatccttgc tgctggcaaa ggaacaagaa tgaagtctaa atacccaaaa gttatacata
    12301 aagtatgtgg aaaagaaatg gttaaccata ttatagatgt ttcaaaaaaa tcaggggtaa
    12361 aagatactgt tgtgatttta ggacatgaag ctgaggtagt taaagaaaag cttgcagaag
    12421 aaattataat agccatgcaa acagaacaac ttgggacagg acatgctgta aagatggcta
    12481 aagaatatat taatgatgaa gatacaatag ttgtactttg tggagatact ccacttataa
    12541 aagaagagac actaaagaga ttatttgaat atcatataga aaataaatat catgctacag
    12601 ttcttacaac aagagttgga aatccaacag gttatggtag aataataaga gataagaagg
    12661 gtgaccttct taaaatagtg gaacaaaaag atgcaaattc agaagaaaag atgataagtg
    12721 agataaactc tggaatttat tgttttaatg gaaagagtct tagagaagca ctagatttgc
    12781 taaataataa caattcacaa ggtgaatatt atttaactga tactgctaaa ataatgagag
    12841 ataaaggatt aaaagttggt gcatttgcag gttcaactat tgaagaactt atgggcgtta
    12901 actcaagagt agaattatca aaggcagaag aaattatgag acgaagaata aatgaatctc
    12961 atatggtaaa tggagtaact ataatagaca ctaactctac ctatatagaa tctgatgtta
    13021 tgataggcaa tgatactata atatatccag gtgttatgtt acaaggaaaa acaaggatag
    13081 gttcagattg tataattgga atgaattcat ctataacaaa ttcagaaata ggtgatggta
    13141 cagaaataaa aaattcaact ataattgata gtaaggttgg agaaaattct actgttgggc
    13201 catatgcata cttaagacca aagagtgact taggaaataa tgtaaaaata ggcgattttg
    13261 tagaagtaaa aaatgctatt atagaagatg gctcaaaagc atctcacctt tcttatatag
    13321 gagatgcaca tgtcggtaaa aatgtaaata ttggatgtgg agtagtattt gtaaactatg
    13381 atggtaaaaa taaatttaaa tcaatagtaa aagacaatgc gtttataggt tcaaattcta
    13441 atttagttgc accagtagtt gtagaagaaa aaggatatat agcaacaggt tcaacaataa
    13501 cacatgatgt cccagatggt gctttggcaa tagcaagaga aagacaagtt atcaaagaag
    13561 gatgggtaga aaaaaagaat caaaaagacg accaaagtaa gtaattatgt agtttattat
    13621 tttggctaaa aaatttagct cgaaattatt ataaattata ttcattagta ttttaaaatt
    13681 ttcaggaggg ttacataaat gaatactagc ggaagcgaga ttaaaattat tgcaggtaat
    13741 tcatcgaaag agttagcaca aaaaatagct gactatatag gtgtatcagt actagattgt
    13801 gaagtaggta catttagtga cggtgaaata tgtgtaaaca tgaatgagac agtaagaggt
    13861 tgtgatgtgt ttgtagtgca atctactaac agcccagtaa atgataactt aatggagtta
    13921 ttaatcttaa ttgatgcgtt aaagagagca tcagcaggta gaataacagc agttattcct
    13981 tactatggat atgcaagaca agacagaaag gctaaggcaa gagatccaat cactgctaaa
    14041 ttagttgcaa atttaataac tgctgctgga gcagatagag tattaacaat ggatttacat
    14101 gcagctcaaa tacaaggata ttttgatata ccattagacc acttactagg tggaacaata
    14161 ttagcaaatt attttaatga aaagaaaata gaagatttag tagtagtatc tcctgactta
    14221 ggaagtgtta ctagatcaag aaaatttgct aatactttaa atggagaagt tccaatagct
    14281 ataatagata aaagaagacc gaaagctaat gtatgcgaag ttatgaactt aataggtgat
    14341 gttaaaggta aaaatgttat actattagac gatatgatag atacagcagg aacaatagtt
    14401 aatgctgcaa atgcacttaa agagtttgga gcaaaagacg tttatgcttg ttgtactcat
    14461 ggtgtgttat caggtccagc tattgaaaga attgctaatt cagaaataag tgagttaata
    14521 gttcttgata ctatacaact tcctgaagag aaaagaatag ataagataaa gataaaaaca
    14581 gtagctcctt tatttggaga tgctataaga atgatattct ctaatgaatc agtaagtaaa
    14641 ttattctaat ttattgtatt taggagagtt gtttaaaagg atgtaaacct tttgagcaac
    14701 tcttttttga ttttatactt atgaaatgtt atttttctct tgttagaaag gtatatatta
    14761 ttgtagaaag aaattatagt ttaaaattat gtatatttaa gcaaaataaa cttaataaag
    14821 ggcatattat tcataatatt gtgaatataa aaaatattac aaaaaagtat tgaaaaaatt
    14881 cagattatgt agtattatgt aaatataaat aaagaaagag gtgtttccaa tgggaaggtt
    14941 agaagatgaa cccctaataa ttaaatgatt cagtcacaat ctaaagtgaa tataaaaatc
    15001 tattgaaagt ttatttataa gaattgaaaa tagtatctat tctatcttta gtgtttgtga
    15061 gagggttctg gattatataa gattactgta aagaagtaat tttgaacaat aagcatatct
    15121 gtaaatgaag agtttcgttt atataatgat tgtggctgaa tcataactat aatagataga
    15181 atgtttataa tagatgattt tataaaatac tatattgtaa tagcttttag ctttcaaata
    15241 aatattagca aaggatggaa atgacaatga taaaactaaa tgataattta taaatatctc
    15301 cttacgatat ataataattg tagggagatg tttctttttt tgtatagtat aattttttct
    15361 agaaattctg tttcaatatg taaaaagttg gtatatataa atagtataaa ctgtgttgct
    15421 aaaatatggt agatattata taatataaat ggatatgtat atacattatg agttatataa
    15481 ttttgaattt aataaaatag aaacgttgat ttatgttctg taatgtgggc accttggagc
    15541 atattgagtt agtggtgcaa ccggctatga aattgtattt atttatagat actattattt
    15601 tcatagcttt ttttatttaa aaaatggtta tatgttagaa ataaatttgg ttagtacgtt
    15661 taaataattt tacttagatt tttaaccaga tatattataa gtaagtatta attaaaaaat
    15721 ttaaggggga ataaaaatga agttaaaaaa gaataaaaaa ggtttcactt tagtggaatt
    15781 attggtagta attgcaatta taggtatatt agcagtagtg gcagttccag ctttatttag
    15841 taatataaac aaggctaagg tagcaagtgt tgagtctgat tatagttcag ttaagagtgc
    15901 agcattatct tattattcag atactaataa gatgccagct acaacatctg ttagtgattt
    15961 aagtgaatta aaaacatata tggatacact tcctgataaa gcagatatag gaggaaaata
    16021 tcaattgctt ttggttgata agaaactagt acttcaaata ggtgataaag atggtggagc
    16081 tacattaaca aaagcacaat cagcaaaatt attgagtgat ttaggtaaaa ataaaatata
    16141 taaagaggct gatttaaaag atacaaatga actaaagaag gatgatgaaa taaataataa
    16201 gaatctatat atagtactta tagataatgc tgatatggat tcagctactg cgtaagacct
    16261 tatgttcatt aggtaagata gtataaattt aatgaaaaat ataatataaa agacaggaag
    16321 aaaatcttcc tgttttttta tactattttc tttaatgttc aattatgaat gatgatataa
    16381 ttgagatagt aaaggagtga aacctgtggc taaaaaagtt agaataggag acaaactggt
    16441 cgaaaaggga tatataacag aagaccagct taaatgggca ctatcagaac aaaaaaacag
    16501 tggaaaaaga ctaggagaat ttctagtaca agaagggctt atagatagta acttactaat
    16561 aagtgtatta aaagaactct tggatataga aagtatattt ttagaaggga cagaaataga
    16621 cactttagct acaaagatgg ttccagaaaa tatatgcaaa agatatactg tatttccatt
    16681 taaaattgat ggaaataaaa tatgtctagc catgtcagac ccacaagata gagaagcagt
    16741 tcaagatgta aggcgtatgt ctggtaaaga cgtagagata tttatatcaa gtacagaaga
    16801 tataaacaaa gcaataggtc atgcttatgc acattcagaa ataaataagg caatgactga
    16861 gtacaacaaa aatagaactg gtggagtaag agaaacagta atacttgaag aagatgtaaa
    16921 tgctgctcca atagtacgat tagtaaataa catactagaa aatgctgtta gaatggaagc
    16981 aagtgatata catatagaac aaagtgaaaa ttacatgcgt gttaggttta ggatagatgg
    17041 aatgttaaga gaatatatga ggatgaactc agcaccatat aaggcagtaa tcagccgtat
    17101 caagataatg tcagatataa atatatctga aaagagaata ccacaggatg gtagaatcta
    17161 tttaaaagta gataataaac ccatagattt tagggtttct acaatgccaa ctaacaggga
    17221 tgaaaagata gctatgaggg ttcttgataa gagtaatttt atggttagta aagaagtact
    17281 aggaatagac gaacatggct ctaagatata tgatgaattg ataaacactc cttatggtct
    17341 tatattggtt gttggcccta ctggaagtgg aaaaaccact acattgtatt caatgttaaa
    17401 tcaactaaac acagaaaata gaaacctatt aacaatagaa gaccctatag agtatgaatt
    17461 acctggtgta aatcaatctc aaataaatga aaaggcaggg cttactttcg ccagcggact
    17521 tagagctttt atgcgtcaag acccagatat aataatggtt ggagagataa gagatactga
    17581 gacagcagaa atagcaatta gagcttctct tacaggacat ttagttttaa gtacattaca
    17641 tgcaaataca gcagttggag caatttcacg tcttttagat atggatgtgg aaagtttttt
    17701 aataacttca tctgttttag gtgtaatatc tcaaagactt acacgaaaaa tatgtgagca
    17761 ctgtaaggtt tcatatgaag cagatattgg agaaaaaaag gctcttggta ttgatgtaaa
    17821 tgaatcaata actatttata gaggaaaagg ttgtgaaagg tgtaataata ctggatataa
    17881 aggacgtcta ggtatatttg aaatgcttga aattactcca gaaataaaag agttaataga
    17941 ttcatctgca aatcaaagag aaattttaaa gatggctaga aaacaaggta tggttagctt
    18001 aaaagaggat atagtcaaaa aagttttaaa tggaaagaca acagtagaag aaatgattag
    18061 aataattttg atgactgatt aaaggatgat attatgaaaa gttttaaata cacagtaata
    18121 aaagaggacg gaaaaaagca aactgatgtg attgaagcca acgattttaa tgaagctaga
    18181 aatcttctta gaaagagaaa tcttagagtt attgaaatca aagaaagcaa aaataaaaat
    18241 ataagattat ctagtagaaa gagaaaaaaa gacttgggag ctgaccagat aagccacttt
    18301 tgcagacagt ttgctattat agtatcttct ggtataaata gtatttctgg attagaaaca
    18361 ttagctagaa ggtcaaccaa cattacatta agagaagaaa ttaatcgtat agtggcagaa
    18421 ataaaagtag gttctactat tgcagattca atgctttcat caaaatcaaa gtttccaaag
    18481 ttactcggtg ctatggtagc tacaggtgaa gctacaggta aattagatga agtattaaaa
    18541 agtatggctt cattttatgc aagtgaacac agagttaaac aaaagcttag aaatgctgca
    18601 acatatccat taatagtttt gatttcttca tttataatga tttttatatt tacaacattt
    18661 atggtaccta agatgattaa ttcaattaca acagtaggag caactcttcc acttataaca
    18721 agaattgtta tggggtttgg tatgtttatg aagaagtttt ggataatagt atttttagtt
    18781 attatattat ttatctatca gtttaaaaaa tatctaaaaa cgccagttgg aagggctcat
    18841 aaagatagag ttataaacaa gatacctgtt ttaggaaaag gaataaattg tatggtagct
    18901 acacgatttt caagagcatt gtatctattt gtatcaacag gatacccatt agttcaaggg
    18961 cttgattata ttatagatag tgtaaataac accatggcag aaaagatttt agcatcagct
    19021 aaagatggaa taacaagagg agaaggttta gcagaaaatt tagaaagata tacgtatttt
    19081 gactctgtat tggtacagat gatagctata ggggagcaga caggggaatt agagaatata
    19141 agtagacaga tggccgaatt ttatgagtat gaatccgaga tttatttaaa taggatggcc
    19201 tcaatgatag agcctgtttt aataattgca gttggtatta tagtttcgat actggttgta
    19261 tcaatattta tgccaatgct tagtatatat gatgccatgt aggagggaaa tgacaaatga
    19321 acaaggttta tgtatcatat tatggcgatt ctatttccat agttgagggc agatataaac
    19381 acgataagga taagttttat gtaaaaaatt ttaatgtgtt ttcaatagag gatatcgcac
    19441 aagatttttc taaggaaaag tatgctctat tgagatatgc attagaaaat gttaagttaa
    19501 aatcaagaca tgtagttttt tgtttaaata caagagatgt aattttaaaa ccccaaaaac
    19561 ttcctaatat ggataaaaag gatttagacg gatttatgaa gctagagatg gacgaaatga
    19621 tggctttgga gtcagatgaa tacgtatttt cgtatgaagt atctagtgaa acacctgata
    19681 attcagatgg cgatgaatca agcttaaatc ttatcattgg agctatacaa aaggaagagg
    19741 taaatgcaat tgtagagata atgcatgaat ttgatttaat attagataga atagatacat
    19801 tatcaacagc ttacttaaga atgcttaaaa atttagatta caatgatata atggtaacca
    19861 atataagtga caatagcaca attataaata tatataagaa agatacttta tttattagtg
    19921 ataatatacc tataaagata actgctaaag atatggacat gcaattatta aatgtagtag
    19981 aagaatcaaa aggtcttatg aattactatt cgtcaagaaa ctttggtaag gttacggata
    20041 ctatattaat attaggaaaa aggtattcca ataaattaat ttatgaaact tttagctctg
    20101 cattttcaag ctatgtttct caaggtctta ctgagataat ggatatatct gatatgataa
    20161 ctggagatat agatgaagat gatataaatt caattgtaga gcttttagga agtatgatag
    20221 aacctaaagg gaaaagagat tttgaaaata taaacttttt accaatggat atacttagaa
    20281 aacaactaaa agctaaacag atgaaaaaca tactgaaaat tgttccaata gttattttga
    20341 ttcttgctat accttattta tgtttaattt tttcaaataa tttagcaaat aaagaacttg
    20401 aaaatgttaa tggtgaaata aaaaatattg aatctgcata ttatcagatt gggaatatag
    20461 aaaaaatgat acagagtaaa agcgaagaaa taaaggtata tgatatgtta attggaaaaa
    20521 aggtaaagtg ggaaaattta ttggatgata tagataaaaa tactccaagt aaagttgaac
    20581 taacaagttt atcaacgact tatcaaaatc ttggaactaa taataaagct gataattcaa
    20641 aaagtaataa gactacagca gataaatcaa gtaacaatga aacaaaatca aatacgaaaa
    20701 gtgatagtaa tggagataaa acaaataatg atttaaatga agataagact acaaatgtgt
    20761 cagaagcatt aactggaaat agtgagacta atagtaataa cacagataaa gatgataaaa
    20821 ctccattgta tgataagata ccaaatgtta taaatataga aggaaatgct aaagattcct
    20881 cttatgtagg tcaattttta tataaattaa agggattgta ctatttcgag gatgttaaat
    20941 tacatagcat aaaaagtaat gcagaagatt ctacctatac atttagtatg acattatacc
    21001 tgaaagaagg tgtattaact aatgaataaa gatatattta aaaaagatat aaaagatata
    21061 tttaaaatgg aagttagtct aaaagggatt cttataaagg ttataatttt attagctatt
    21121 ttatcatatg gttattttgg actttaccct caatatcaga aatatattgc tacaaaaaat
    21181 tctgtaaaac aagctactga aaaattaaat atatataaag agaaaactgc acttatgcca
    21241 aaatatgaaa aacaacttga agaagttgaa agtgaattaa aagaaaaaag caaaaatctt
    21301 ttatatgata tgcaagatgg tcttttttta ataggtttag ataaaaaaat gaaggcttat
    21361 aatattgact taactagtta tgacgttgga aatgttagag aatacgaaca tttttatggt
    21421 gtatcaacaa caataagtgt tagaggtgat tatagaaatg tcaaaaaatt aatggcttat
    21481 ttagaagaac aaaaaaatgt tactcaaata ctagattata caatgacttc atatatagaa
    21541 ccagaaaaag aagaggaaga aacaacaaac aataaggatt taattaaaaa tgtgaacata
    21601 aataaagata caaaaatata ctgggtggaa ggcgaaaata agtggcattt atataaggat
    21661 tgtccattga taaaagaggg agagattcta aaaaatgaca aacttttaga taggtctact
    21721 aattatctat gtaatacttg tgtacttaga gctgcaacag aaaaagaagc aaaaaaagaa
    21781 gtcgtagctc caaaggcaga aggtgtagta gatgctacat ttaaatttat aatgtacact
    21841 aaagagaata ctacaatgat acttgataca gataatccat cttcatggag accaggaaaa
    21901 tataatccat ttaagtctag tgcgaaataa agctaagtaa gctttaaaga taggtgatga
    21961 gtttgtacaa aaataatgaa aaaggtctta ctttattaga agtaataata gctgttttta
    22021 tactgacaat agttttaagt atttcttata aagtgttcaa tggtataaca tcagcagtaa
    22081 aaaagcaaca gattattaca gatgctcaag taaatattaa tctaattaat aagtatctaa
    22141 atagagattt ggaaaactgt aaagaactaa ctaaaactgg ttcaggtaat aattatgaat
    22201 acaatataga gatgccagat aatgttgtaa aatatgaagt ttctatagaa actaaaaaaa
    22261 atacagaggt atattctgta acgagaatcc aaaacaatac aattgataca gaaaatgaag
    22321 ttagagaaga gataatctac aatcaaccac tagttcaaaa caataaagaa atgaaggaaa
    22381 caccatttaa aatagaaaag caaactggta aatctattta tactgtaagt atatactata
    22441 atgaatcagt gcaagaaagt cataaaaata gcaatttaaa taacaaaact tatacatttg
    22501 atgttatgtc aagaataggg taataagaat ttattaaaaa ataagtaggt tgataaaata
    22561 tgagtaaaaa gaatagtgaa agaggatttt ccttaattga agtgttagta gctatggcta
    22621 ttatgggaat tgtactattt gcatttttta atattatcaa tactaataac aaagcaaaca
    22681 ctaaaaatga cacagatata acttccttaa actatgtcca aagtgaaata gaaaatctaa
    22741 gagaaaagat aaaaagtgga gaatttgatt ttgatagttt agataaacta gaagatggaa
    22801 ctgtcgtata tgaaaaatta atagataaat caaaaaaagt tgtatatgat aaagttctta
    22861 gtgaaggtga tgtgagctta tatgatactc catatgaaaa gattacaaca ataaaagatg
    22921 aagatggtaa tttaatcgac aaaggaaata ttacaaataa gataaagact attgtagaag
    22981 ataaaagtgg tcagatatat aaaatagctg taactgggaa aagcatgaat gactattcaa
    23041 gtaaaaaaga ggttaaaatt gtaactgaaa tatttaaaga taaataattt tacttggggg
    23101 tgagattttg aggaagtgga ataaatttaa aagtgaaaga ggggcagctt tagttttagt
    23161 tttggttgta gtagcattgc tttctattgt tggattgata ttttcaaatc agatagctaa
    23221 tagaataaaa tctacaaaga ctactaatga aggtatacaa gcaaagtatt tagcagagac
    23281 atgtgtggaa aatagtattg ataaagcata tgaaaaattg tatgatgagc ttgagaaaat
    23341 ggataatgag tttaaaagtg aaaatcaaga aaaatccata tctaggtcaa aattaagaaa
    23401 tatatcagat gaagatttta ataatcaaga tgaggaaaat attgaagcag aaagacttgg
    23461 atatatgaat aatataaatt tctatttaaa taaagcttct tcagacttgg aaaaagctag
    23521 tatggaattg aaaaagctat atgatttaga tatgttggac tatagagaca ttgaatatgt
    23581 agatgcaaat attatttcac atagagattc tatcttagaa atttgcaaga attatacttc
    23641 tggagatatt agtaaaataa atgaatatat tctgaaagaa gatatagact caactacatt
    23701 gattgaggca aagttagtga ataatgatat tcttttgaaa atgtttttag aagaaaataa
    23761 aattgaaaat gaacatttaa attcagcctt ttcacatact tataaggcat tagataacat
    23821 ttctttggcg atgcaaaata tgattgagta tagacatacg tttcatatag atgagccaaa
    23881 agtagaagta tctaatggga ttccagactc tcaacaatat tatgaactaa tacaaaatcc
    23941 tataataaat agtatggagt atagctggaa tagtaaatgg gatacattag aaaacctatt
    24001 ggaaatttta ccaaatcaaa ctcaaggatt taattcttta agagttcatt tgagaaataa
    24061 tgtaagaaaa tttgagaagt taagcgataa tatatctagt ggtaaaaaaa atacagcaaa
    24121 aaatttctta aagtataaag agttattgta tgaaatatca gaccaatgta atcaactaaa
    24181 aagtatgtct tatgagaaaa tacctgttaa atatgataac atggctttga taacaacttt
    24241 tgactatata caaaatgagc ttttagcaga gataaaatgt agattaaaag aattaaaacc
    24301 acaagaaatt gataaaacag aaggtattac tataaaaata cctttttata aggctgacta
    24361 cgacatgaca aaagaaggat ggcctaaatt aaaagaaaat ggttcagggg cggagttaag
    24421 cttaatggta actggtgata aagatggaat aaaagaagta gaagttacag atggtaaaaa
    24481 aaatattatt ggtttaggtg ttgaagaaaa ttcaaattca aagtataagg ttgacgccat
    24541 tgtaaacttt aatctaaata tagatacaaa tgtagttgga aattatgata taaaagataa
    24601 aattttgatt aatcatgata taagttcata caaaaaagta aactaaatat aagaagcaac
    24661 ttagtataaa taacaaaata attttattat ttactactaa aaaaataagt aaactaatag
    24721 aagatatgga gagtaaatat tatgtatatt tatgatttat tagagcaagg tataagacta
    24781 aacgcatcag atatacacat aacagttgga acaaatcctg tagctagagt aaaaggtgga
    24841 tttgtaaaac taagtgaaca aatattgaca tcagaagtaa caatgcaaat ggcaaaagac
    24901 atagcagggg aatctatgtt taaagtaata gaagaacatg gagaagctga tttttcagct
    24961 tctctaaaaa caggagaaag atttagggta aatgcatata ggcaaaaagg aaattacgca
    25021 atagcaataa ggactataac agcagaaatt ccaacatttg aaaaattagg ccttccagag
    25081 agtataaaat cttttactga aaagcataaa ggattagttt tagttacagg tccaacagga
    25141 agtggtaaaa gtacaacact tgcaagtatg ataaatataa taaatgaaaa acaacaaaaa
    25201 catataatta cacttgaaga cccgatagaa tatgtacatc atcataaaca aagtttagta
    25261 aatcaaagag aagtaggaac tgatacagaa agttttcatt cagcccttag agctattttg
    25321 cgtcaggacc cagatgtaat actaatagga gagatgagag accctgaaac tgtttcaata
    25381 gcattaacag cagcagaaac aggtcattta gtattttcaa cactacatac agtgggggca
    25441 gctaagacaa ttgatagaat agtagacatg tttcaaccat cccaacagca acaaataaaa
    25501 acacagttat ctacagtttg tgaaggtgtg gtatcacagc agttacttcc tacagcagat
    25561 gggaaaggca gaatagcagc aattgaattg atgtttgcta ctccagcaat aaaaaacctg
    25621 ataagggaag gcaaaacata tcaaattcca aacatgatac aaactggagt taaagtaggt
    25681 atgaaaacta tggaccaaga tttaatggaa ctttataaaa atggaaagat tacaaaagat
    25741 atggcactta gtaggtgtac tgaccaagag tttatgacaa gaatgatagg tggagtaaac
    25801 tacaatggat attataatcg ttaatctata tgtatttata gtgggtatag tgtttggaag
    25861 tttctttaac gtctgtattt ttaggattcc agaaggtata tctatttcta gacctccttc
    25921 tcattgtatg agttgtaata ctagactaaa gccaatagac ttaataccag tgttaagcta
    25981 tttaatgagt ggcagaaagt gtagatattg ttatgagaaa atttctagta gatatgctat
    26041 agtggaatta attacaggac ttttattttt aacagtattt atgttatatg gaatatctac
    26101 atcaactatt tattatttgg ttcttgtgtc tttactgatt ataattacat ttatagatat
    26161 tgaccacttt ataataccag ataaaatatt ggtttttgga gctatatttt ctatgatttt
    26221 taacctttta tttaaagaaa ttccagttaa ggattcaatt ctaggtgttt ttatttgtgg
    26281 aggaagtgtc tggattatag ttcttttgat tgaatttgta ataaaaaaag agtgtatggg
    26341 tggaggagat atcaaactat ttgcaatgct aggtttttac atgggtgtta aaaatggtct
    26401 tttgacagcc ttactaagcg tttatgtggg agcagtctat ggaatctgtg taataattat
    26461 aagtagaata aaaggaaagg tatataattc tgttatacca tatggacctt ttatatctat
    26521 tggagcatta ataacaatct tatgtggaaa gcaattactt gaactttatt tttctatggt
    26581 tatatagatg atagtcaagt taaataaagt tttaatttaa aaaaatgaat taaagtattt
    26641 aataaggaca atattattat taaccctatt actaaagtgc tacaaactat ggagtatata
    26701 ataataattt ccttattttt tgcacatttg ataaataaat ctctatgtgt tttttgtgat
    26761 agagaaaaga aaaatttata taatacgttt tttatttaca taataactgt agttttatct
    26821 gtattaatat atctaaaatt tgggattagt gtagaatgtg tacaatatat ggttttaatt
    26881 ccatttttaa taattatatc cattgtagat tactacacaa tatatgtata tgatataaca
    26941 atagttagtg gtataatagt tcaaggtata atttttttgg tctttttcaa tatagttcaa
    27001 tttgattata taaatcatat atttggacta ttgataggct ttattatacc ttatattatt
    27061 gctaaactaa caaaaggatt aggctatgga gatgtaggtc tctatagctt atgttgcttt
    27121 gcattgggaa ataattatgg gttttatgtg atagttttat catttatttt agcgtttttg
    27181 ttttttatta ttttatacat aactaaaata tttaaagatg tagatataag aaagcaaatt
    27241 ccttttacac cttttatttc cttgtcaaca attttaataa tatttacaga gtataatata
    27301 tttaattttt attataattt gttatacaaa tttatataac aggaggtaag aaatgtacgt
    27361 agtagtaggg ttaggtaatc caggaaagaa gtatgagaag actagacata acgttggatt
    27421 tgatgttata gatattcttg ctaaagaata caatattagt gttactaaaa taaagcataa
    27481 agctttgatt ggtgaaggga gagtaggtac tgaaaaagta ttattagtta aaccacaaac
    27541 ttatatgaat ttaagtggag aaacattaat agatatatat aaatattata aggttgattt
    27601 aagtaatata gtagtagtat atgatgatat agatttagaa gttggaaaga tacgtataag
    27661 aaaaaaagga agtggaggaa ctcataatgg catgaaatct ataactaagt gcctaggttc
    27721 aaatgacttt ccaagagtga gagtaggtgt atctaaacca gaagctggtc aagacttagc
    27781 agattttgta ctttctagat ttagaaaaga agaatctgat aacataaatg aagcattaga
    27841 aaaagcggca gatgctattg actcaataat aagagaaaat atagatatgt ctatgaacaa
    27901 atataatgga tagtgagagg gagatttaaa tgaatgatgt ttttttatat cctttgcaaa
    27961 attcaaaaga gtataaagat ataataaact gtataaaaaa tacaaaaggc tcattattgg
    28021 ttaatggact tttaccagta caaaaacctc atatatcgta ttctatattt aatgatttat
    28081 cacgacaaat gatttttata acgagtagtg atttagaagc aaagaaagta tacgaagatt
    28141 taagttttta tatggaagat aaagttgaat atttaggttt tcaagatatt tatttttatc
    28201 acttggatgc aaaggacaga aacgaagaag caaagaaatt aaaagtattg ttaaaactag
    28261 caaataaaga aaaaatcatt ttagtaactt ctattgaggc agttttaaga aaatacatac
    28321 caaaacaagt tttgctcgat agtgtatctc attataaggt gggagatagc ttagacttag
    28381 aaaagttaac tgagaaatta gtttcattgg gatatgaaag agtttcaaaa atagaggggt
    28441 ttggtcaatt tagtataaga ggtggaatta ttgatgtatt ttcacttgaa tatacaaatc
    28501 caattcgtat ggaattattt gatgatgaga tagattccat acgaactttt gacgtatatt
    28561 cacaaaaatc aattgataaa ttgcaacaat tctctataac tccatctagg gaatttattt
    28621 atcccgaaaa aactacagat gcattagtta aattaaaaaa agaaacaact aaaaatactg
    28681 atgaaggtac atttcaaaat atagattata tatcaagtaa gacttatttt gaaggtgtag
    28741 agaactatat agattatatt tatccagaag aaaacaaaag tatatttact tatttagcag
    28801 atgatgcaat agtatttatc aatgatataa cgagacttaa agaaagatgc gaaaattata
    28861 taaatgagtt tagagaaaat tataaattaa atcttgaaag aggtctggct attaaaaacc
    28921 aaggtaaatt gttatatcac tatacagatt tagagtactt agtaaaagac aagtctgtgg
    28981 tattaaactc gctattgcca aagtctataa ataacttttc tataaaatct ataataaatt
    29041 ttgaaagtag agaagttcct acatttaatg gaaaggtaga tttgcttgta gaagaattaa
    29101 atcggctgaa atataatgga tacaaaataa ttttagcaac aaatacctta gaaagagcaa
    29161 ataaattagg aaaagattta ttggacaaag gcttagaaac gaccatttct aaagatagag
    29221 atattgaaat taaatcttca caaattataa ttgtacctgc acacataaac agtggttttc
    29281 agtataaatc tataaagttt gttgttataa cagataacga aatgataggt gtttataaaa
    29341 gagcatctaa gactagcaat aaaaaagtta aaaagggaaa gaaaatagaa tcctttttag
    29401 atttaagtgt tggagactat gtagttcatg aaaatagtgg tgtaggtaga tacacaggaa
    29461 ttgaacaaat tacagtcaat gctattaaaa aagattatat gaaaatagta tatcaaggtg
    29521 gagataatct atatgtgcca atagaccaga tggacaaagt acaaaaatat attggtgcag
    29581 aagtagaaaa agttaagtta aataagcttg gaacaaatga gtggacgaaa gcaaaagcaa
    29641 aagttaaaaa agaaattgaa gatatgacaa aagacttgat tgagctttat gcaaaacgtg
    29701 aaaaaataca agggtataag ttttcaaaag atacaccttg gcaggcagag tttgaatcgt
    29761 tgtttccata tcaagaaaca gaagaccaat taaaggctat agaagaaact aaaaaagata
    29821 tggaatcaag taaggtaatg gataggctag tatgtggaga tgttggttat ggaaaaacag
    29881 aggtagctat tagaagtatt tttaaagctt gtatggacca aaaacaagtt gctgtattag
    29941 ttccaacaac aatattggcc caacaacatt ataatacatt caaagagagg tttgaaaatt
    30001 atcctttgag agtagaagtt ttaagtaggt ttaaaacacc aaaacaacaa aaacagatta
    30061 ttgaagatgc taaaaaagga ttagtagata ttttaattgg aacacataga ataatatcca
    30121 aagatataaa tttaccaaac ttaggtcttg tagtcattga tgaagaacaa agatttggag
    30181 taaaacataa agaagcttta aagaaaatta aatctacagt agatgtactt acactttcag
    30241 caactcctat accaagaaca ttacatatgt ctttaagtgg aataagagac atgagtgtta
    30301 tagaagaacc tccacaagaa agacatcctg taataacata tgttacagag agtaaggaaa
    30361 gcgtaattca agatgagata gagagagaat taagtagagg tggacaggta ttttttgtat
    30421 acaatagagt tgaacacatt gaagaaatgg caagtatgat tcaaaaactc gtaccagatg
    30481 ctagagtggc tgttgcacat ggaagaatga cttctaaatc attagaaaat ataatcctag
    30541 gatttttaaa taaagattat gatgtactag tctgtactac aataattgaa actggtatgg
    30601 acatatcaaa tgcaaataca atgattatat atgatgcaga taaaatggga ttggctcaac
    30661 tttaccaatt gagaggtagg gttggaagaa gttcaagaca aggatatgca tatcttttgt
    30721 atgaaaaaga taaaacttta agtgagatag cggagaagag attaaaagct atcagagaat
    30781 ttacagagtt tggttctggt tttaaaatag caatgagaga ccttgaaata agaggagcag
    30841 gaaatatttt aggttctcaa caacatggtc atatggcagt tataggttat gatttgtacg
    30901 ttaaaatgct taatgatgca attaaaaaag tcaaaggtga acctattgtg gaggaaattg
    30961 atgttgaaat agatttaagt gtaaatgcat acattccaga taattatatc aaagatgagt
    31021 tgattaaaat tgaaatgtat aagaaaatag cttctattga aaacaaagaa gacatgttgg
    31081 atatacaaga agaacttgag gatagattct ctgatatacc taaaccagtc cagacacttc
    31141 ttacaatagc ttatataaaa tcgttatgta agatattaaa aatagaaaag ataagacaat
    31201 taaaagatga aatattatta gttccaataa caaagtatag aactaaacag aaaataggtt
    31261 ataatatagt tacagaatta gaagaattat tagaaaagat gtgcaaggta aaataacttg
    31321 taaatttaag aagggatgtt ttaaatttga aaaaagtaat aacattggta atagctatga
    31381 ttttggtagt atctgtgact gcttgcagtt ctagtaaagg agaaactgtg gcaacagttg
    31441 aaggtacaaa gatatcatca gatgaattta aaaaaactat agctctttat aaagattcta
    31501 tggaacaaac ttatggaaaa gatatatggg ataaggaagt tgaaaagggt gtaaaataca
    31561 aggacaagtt taaagattta attcttgacc aacttataac tacagaagta atatattctc
    31621 aagctaaaaa agataactta cttcctaaaa aagaggatgt tgaaaaaagt tttaaagaat
    31681 taaaagacgc tatgggtaaa gatgaaaaat ataaagaaca acttaagaaa ttaggtatag
    31741 atgatgagtt tttaagagac caacaagaga aagatttagc aatgcaaaac tatcaaagta
    31801 actttgctaa aaagactaaa atatcagatg aagaaatgaa aaaatactat gatacacata
    31861 aagatgaatt taaaaaagat gaggtagaag cttctcatat tttattgaaa acagtagatg
    31921 ataataacaa gccactttca gataaggaaa aagctgaagc taagaaaaag gctgaagagg
    31981 ctcttaaaga agttaaatca ggtgaggatt ttgcaaaagt agcaaaaaaa tattcacaag
    32041 atacttcagc aagtgatggt ggtaaattag gattcttttc aaggggtcaa atggttgctg
    32101 aatttgaaga tgctgctttt tctatgaaaa agggtgaagt atctgattta gttgaaactc
    32161 aatatggata ccacataatt aaagtgacag acagaataaa tgagcaaaca tcatttgaag
    32221 atgctaaaga aactataaaa gaccaactgt tgaaaaataa atatcaagaa caaatagaaa
    32281 aattaactaa agaagctaag gtagaaaaag atgaaaaagt aataaataaa atcacaatct
    32341 aagacttaaa aaaagagtaa tatgcttaaa aaagctatat ctggataaga tatagctttt
    32401 tttgtatatt tttcttaaca atggtaaaaa taaacttaaa gtaatttatg gagggattaa
    32461 aaaatatgaa agcaacaggt atagttagaa gaatagatga tcttggaagg gtagttatcc
    32521 caaaagagat aagaaagaca ttaagaatta gagaaggaga ccctttagag atattcacag
    32581 caaaagatgg agaagtcata cttaaaaaat actctccaat aggtgaatta aatgaatttt
    32641 cgcaagaata tgctgaaaca ttagctgaag tactaggata tggagtgttg ataacagact
    32701 tagattctat aatagctgta tctaagcttc ctaaaaaaga ttataaagaa aaaagtataa
    32761 gtgatgagtt agaagcaata ataagtgaaa gaacagtaaa atattcaaaa gataaaaaca
    32821 ttttaccatt attcaaggaa gaccaaaaag aatatactag tcaagttatc atgcctataa
    32881 ttagtgcatc tggtgactgt ataggttcaa ttgtagttgt ttcaaaggat aaagatacaa
    32941 ttgcagaaag tgaagaaaaa tctttaaaaa ttgctgctaa ctttttagga aaacaagttc
    33001 aataatttta tcataaagac cttttacatg aaggtctttt gaagtttttg tgtaaaatat
    33061 tgaatatagc attaaaaata tggtatatta aagtttgtta tataaacagt atggaggtta
    33121 tgttatgaac aataacaaaa ataaagactc ctttctaaaa ggagccctta tacttgggct
    33181 agcaggtgta atcgtaaaaa taatgggtgg attttttagg attccccttg gaaacatgat
    33241 tggttcagaa ggaatgggat actatcaggc tgcttatcct gtgtatactc tatttttaac
    33301 attagctact gctggatttc caacagctgt ggcaaaattg gtttcggaaa aagttgcaat
    33361 tggaaatttt aaaggtgcaa ataagatatt taaggtatca catacagttt tattcattac
    33421 cggtataata tcattttgta tattattttt tggagccgac tacatagtaa caaacgtaat
    33481 gaaaaatcca ggtgcgttat actctatgaa agccattgct ccagcattac tatttgttcc
    33541 tgcaatgtct gcatatagag gatattttca agggagacaa gatatgacta agattgctgt
    33601 gtctcaagtt gcagagcaat tttttagagt tgtattaggt cttactttgg cctacttgct
    33661 tatgaagagt ctaggtcaga cttttggtgc agcaggtgct atatcagggg ctacaatagg
    33721 ttcaatagca tctatgcttt atttggtgtt tgcttatatg ctagggagaa aagagcgtag
    33781 agcagagata gatgcaagcc aaagatttaa agatgaaaga gtgtcttata tttttaaaaa
    33841 gcttttaact gttgccattc ctataacaat aggagcatca gtaatgccac ttgtaaatat
    33901 gatagataat gttattgtaa taagaagact tatggaagca ggctttacat acaaagtggc
    33961 aaattccatg tttggtcaat taacaggtat ggcaatggcc acaataaatt taccagcagt
    34021 cataacaact gcaatgagta tgagcttagt tccagcaatt tctaaagcat atgcattagg
    34081 aaataagtca aaagctagaa aagatactaa gagtgcagtt aaggtaactt tacttatagt
    34141 attgccttgt gcgtttggta tggcttcttt agcaatacca ataatgggtc ttttatttcc
    34201 acatgaacca tcttctgtag gaactatttt gtttactctt actccatgtg tattattttt
    34261 aggattaata cagacattga ctggtataat tcagggaatg ggaaaaccaa tagtacctgt
    34321 aattgcttta tgtgttggta tgctatgtaa aattgtaata agttatactt taacaggtat
    34381 accagatatt aatgtactgg gttcagcatt tggtacagtt acagcatact ttgtggcagc
    34441 catgattaac ttactatatg ttaaaaaaca catgaatgta aacttttcta aaaaagaatt
    34501 tataataaag ccatttataa cagttatgac aatgtttata atggttaagt tgagttatgg
    34561 tgctttagtt ggattcttag gaaacagcat atctacaata attgcaatat gtgttggtgg
    34621 aattgtatat gtagtagtaa tacttggaat aggtggaatt aaaaaagaag agattctaac
    34681 tatgccaaaa ggtgataaat tatataaatt gttaaaaaaa gttaaactta taagataata
    34741 taggaatata tatgcaaata aaggaaatac tatacaaata gtaaatctcc ttatttgtat
    34801 aataatataa aaataaagtg aaatttgcta ataggagtga tgttatgggt aagataagta
    34861 ttgtaggtct tggaccagga gactattctc ttataagcca aggtgtgtta gaatctttaa
    34921 taaatagccc taaagttttt tttagaacag agaaacatcc aataattgat aaacttaaag
    34981 aaactataca atacacttct ttagactact tttatgaaaa agaggaaaat tttgataatg
    35041 tttatttgaa aatatctgaa tttatcatag aagaatcagc aaagggtgac ctagtgtatg
    35101 ctgttcctgg tcatccaaga gttgcagaga agactgtaag tataatagag agattagcca
    35161 atgaaaagca catagcagta gaaatattgc cttctatgag ctttgtagac gctatgttta
    35221 actatttggc tatagaccca tctgagggat ttaaactggt agatgctttt gaaatagaaa
    35281 attcatatat agacttggat acaagcatga taataactca ggtatatgat aaatttatag
    35341 catctaatgt aaagttaaaa cttatggaac attatgacta taatcaagaa gtatgtatag
    35401 taaatggagc aggtgtcaaa gatttagaaa gtaaaaagtt tataaagctt tgtgaattag
    35461 acagaaatga aaactttttt gactatttaa ctagcttata tatacccaaa agttctaaaa
    35521 agatgtataa tacagtacat gacttagaaa taatagttaa tacacttaga agtccatctg
    35581 gatgtgaatg ggataaaaaa caaacacatc aatcattaaa aaattctgtt atagaggaag
    35641 catatgaact ttgtaatgca attgataata atgatataga tgaaatggta gaagagttag
    35701 gtgatgttct tttacaggtt atattccatt gtcagatagg aaatgaggag ggctactttg
    35761 atttaaaaga agtagtaaat ggcatatgta aaaaattaat tcatagacat cctcatatat
    35821 ttagtggtgc aaaattagat atgtctaagt ttgagaaaac ttgggaagaa ttaaagaaag
    35881 aagagaaagg cgaaagtact ataacagatg gccttagaag gataccaaaa catttaccag
    35941 ctcttatgaa ggctagtaaa gttcaacata aagcagcact tgtcggcttt gattgggaca
    36001 atgtagaaga tgtatttaaa aaaattgaag aagaatatca ggaattactt gacgaacaca
    36061 gggtcgggaa tataaaatac ataaaggaag agctaggcga tttattattt tcgatagtaa
    36121 atctagctag atttttagac atagactcag gagaagcctt gaactgtact acagaaaaat
    36181 tcataaatag atttgacttt atagaaaaaa gtgcaatgaa cttgaataaa aaattagaag
    36241 acatgacact tgaagagatg gatgagtttt ggaatcaggc taaaaaacaa taaaattgtg
    36301 gaaggaaatt tgtcaaaaat ctagaacttt tatcacaaag atagaagtct atattataaa
    36361 catttttagg aggtaaatat cgtgaataaa gctgaattag tatcaaagat ggcagaaaaa
    36421 agtggattaa caaagaagga agcagaagct gcgttaaacg catttatgag ttctgttcaa
    36481 gatgcactag taaataatga aaaagttcaa ttagttggat ttggaacatt tgagacaaga
    36541 gaaagagctg ctagacaagg aagaaatcca agagatccag agcaagttat agatatacca
    36601 gcttctaaag caccagtttt caaagctgga aaaggattaa aggatataat aaatggataa
    36661 ttagaaatct tgtgaaaaag tgtggataaa tttgccacac tttttctata gaaccaagga
    36721 aggtgataat tatgagatta gataaatttt taaaagtatc taggattata aaaagaagaa
    36781 ctgttgctaa agatgcatgt gataaaggga ttgtcactat aaatggaaaa gttgctaaat
    36841 cttcttcaga ggtgaatatt ggagatacat tagaaataca atttggagag aagaagatga
    36901 agtttaagat aaatgaagta aaagagcatg tattaaaaaa tgatgcaaaa gaaatgtacg
    36961 aaattttaga ataaaattaa tagtcctcta gtatattaat agtagtatta ttgaaatatt
    37021 tactgggggg atattatgga acagaacata actttgaaag atagatcaaa gctggttatt
    37081 tcgggagtcg agcacatata ctcttttaat gacaaaagag tagagctcaa aacttctgta
    37141 ggcgagatgg taattgaagg cgaaaaccta gatatgagca agctaagtat agacgaaaat
    37201 ataattagca tagatggaac tataaattct atggtatatg caaagcctaa aaagccacaa
    37261 gagagtttct tgaaaaaggt atttagatag atgatacctt ttactcaaga tgtaagcatt
    37321 ttttatgcta ctatttatgg tggaatactc ataggggtgt tgtttgattt ttatagaggg
    37381 ctaagaggga attttaaatt catcaattat tttgctataa tctttgatgt attgttttgg
    37441 tttctagcta cggttataat attcgtaaca ataaacctta cagaattttt tgatttaaga
    37501 tattatcatt ttgtggcttt gtttattggt tttattttat actataacac cataagtaaa
    37561 attgttttat caattatcaa taaaataatt cgttttgtga gaaattcttt taaaaaagtt
    37621 acacattaca tagtcagttt tttaaataat ttgtattacg ttataatcta ttcactacac
    37681 ctactatttg atattatatt ttatatacca aacatattta tagcaacaag aaaaagtata
    37741 aaaagaaaat caaatgaaaa attaaagaac aagaaaaaat caaaacctaa gaagaaaaaa
    37801 aataaaacta aaaaaagggt gtaggaaaaa tgaacttaag aaaaaaattt tcaggacaga
    37861 ttattgttat cagtttattt ttaggaatat caatttttag tatgatgaca ggatttgtat
    37921 ttgagtatac aaaagctaag gaatataaaa aagaaatagc aagtctaaat aaacaattaa
    37981 aaaagactga aatacaaata aacagcttaa agaaagatga aaaatcatat gaaggagact
    38041 tagaggatat agcaagaaaa agattgaata tggtaaaacc aaatgaaaca gtttatgtag
    38101 acataaatcg ttgattaaaa cttgctaata agtttacaga tgtattgatt ttaaaattta
    38161 agatttaata aaaaagtcat tcagttaagg atggcttttt gtttttattg aggagggaat
    38221 ttcataatga aagtaggagc tatagatatt gggacaaact caatgagatt attgatagca
    38281 gattatagag atgggaattt tataaacaga aaaaaatttg ttaatacaac tagaattggt
    38341 tatggtgtgg atagtaaagg atatataaaa gaagatgcta tcaaaagaaa tataaaagca
    38401 ttaaaagaat tttctgataa gtgtaatgat gaaaagtgtg aaaaaattta ttgtatgggg
    38461 acatctgctc ttagagacag taaaaatgga gattactttg ttaaattagc taaagaagaa
    38521 actggaatag atgttgatat agttacggga atagaagaat caaatttggg ctttaagggt
    38581 gttttagaag gaatacaagg tgatgatact attattgtta tagatattgg aggaggttca
    38641 acagaattta ttgtaggtga ttcagaagga attaaattta ataaaagtga gaatgtaggt
    38701 gctcttagga tgacagagag gtttttgaag cacgatatta ttgaagatga agagtttgaa
    38761 gctatgagtt catttatata tgatgagatt gacaaaacgt taaatcatat aaaagacaga
    38821 ggattaaaaa ctcttatagg tataggaggt acgattacat cattatcagc aatgaatcaa
    38881 gaattagaga tttactctat ggaaaaaata cataatagta aaatacacaa aaaagaaata
    38941 gaagaaattc tacaaaaatt aataaagatg acattaagtg ataaaaagaa cttaaaaggg
    39001 ctacaagaaa aacgtgcgga tattataact gctggtgtaa aaatattgaa tataataatg
    39061 gaaaaattag aaatagaaga aataaccgta agtgaatacg ataatttgga aggcttaata
    39121 tgtcaaaagt caaaaaaaat gtattaatat tttttgattt ttaaaaccga ttatgacaaa
    39181 aaaaattact cctctttgtt acaattctta atataaacaa aagaggaggt ttttttatgc
    39241 aaagaagtgt agcagttgtg aagaataaga ctgtaaaacc aaatgtcata aaattaataa
    39301 gcggatatat tgattttact acggcggcat tcgctgtgat gggattttta cttagtaggt
    39361 ctattatagt agactctgta gctcctttag gaattgcctt ttttatatgt gcatcaaaga
    39421 ttgataagta caagatacct gtattcatat caacattatt aggaatattg ttatcatcta
    39481 atgattttgg aaatgtagca aaatatgctg tgtgccttac aatatttatg gctatgaata
    39541 aaaaattaaa attatcagat tcaattgcta aggttgcatt tataggtgca ataatactac
    39601 ttccaatctc catgggacaa gctttctttt caaataggta tgtatatggt cttcttatag
    39661 caggagtaga atcaataata atgtttatat ctatatatgt gttttcttat gggataggtt
    39721 taatggccaa tgtcaacaat aggatgtctg tacggacaga agaagccata tcaataagtt
    39781 tactgctaac ctttagcata atgggaatag gagatatatc tctgtttgga atatcaataa
    39841 ggaccatttt atcgaccgta ttgatattgc tgacctcaat agtaggtgga gctaccatgg
    39901 gagcatctag tggtgtaatt attggtgtag catccatttt aaataatatg acatcagctg
    39961 tttatatggg gatatattct ttttcaggat taataagtgg agcatttaat aaaataaata
    40021 aatatttctg tatactagga tatattctaa gttggacaat aatataccta tatacttctg
    40081 gtattacttc taatatgatg caattaagag atatactatt aggttgtttg atagtactag
    40141 tattgccaga gcgattattt aataagattg aaaaattaat aaaaagtaat gtggcttcaa
    40201 atgaaatcgt atacgattat attatgagaa gcaaaaacct aactaatagc agattgaaca
    40261 gtatttataa aacttatgat gaccttgcag atacatttga taagataaga gaaaaagaca
    40321 aagtgctaga ccaaagagac atagctaatg taatagatat gattcataat gatgaatgta
    40381 aaagttgtag tatgagaaga atgtgctggg agtcaagatt taatcataca tacactatga
    40441 tatataacat tcttgaaaaa attgaggaga aaggggagtt aagtctaaac gatataccta
    40501 aaaattttag aaaggaatgt atgaaaccag agtcaatagt gaaaatatca aatcactatt
    40561 acaaaatgtt tgtattagat tatgattgga gtgtgaagtt ttcagaaagt agaaagttaa
    40621 tagcaaatca gataagaagt atttcaaaat ccattaaatc cttatctcaa gatttagaag
    40681 gcgacataat gctagatata gaaaaggaaa aaaatatata tgaacagtta gaaagatatg
    40741 atataacagt tgataaagta agttacttaa caaaaagtaa cagtgagttt gaaatatcaa
    40801 tagaaaagaa aacttgccat gatggatgta tgtgtgaaga taaaattgta aatataattt
    40861 cagacctagt aggtgaaaat atgtctgtta gaaaaatagg atgtcactgt ttaggaggta
    40921 aatgcaaggc aacattcgtt aaatctcaaa aatataaggc tgttacagaa gtttcagcaa
    40981 tgtcaaggga tgggcatatc ctttgtggag acaattacac atacatggag ataaatgatg
    41041 gtaaatacat gatggctata agtgatggta tgggaaaagg taaaaaagca tatgaagaat
    41101 cttctgccac tattgatata ctggaaaaaa tgatagatgc aaaaattaaa gatgagatag
    41161 tcattgatac tataaataat atgttgttgt taaaatcttc agaagagatg ttttcaactc
    41221 tggatttggg aattttagat ttaaaacgag gttgtttaga aactataaag atgggagcgt
    41281 gttctactta tataaaaaga gaggatggag aagttgactt aatatcatca tcatcattac
    41341 ctgtgggtat attatcagat gtaaaaattg atagaaaaaa tgtaaaagtt aaggaaggag
    41401 actatgttat aatggtttct gatggaattg tagatgcagg aagaaataac aatttaggag
    41461 acaattggtt aatatacttc ttaaagaata tagagactac aaatccaaag gaaatttcaa
    41521 atttaatatt ggatagagca ttagagttac aagcgttaca aatcgaagat gacatgactg
    41581 ttttagttac gaaaatatgt acaaaataag atttacaaga ttatgtgtat aagatgtgga
    41641 taacttgtgt ataatatgtt aatagacttt tgaaaaaagt atagttatta acaaaaataa
    41701 catataaatc aacaaaattt gttgataagt atggcagaaa tgttaataaa atgtaaaaaa
    41761 agaaaaaaca aaagctatta tcaaaagaga ttcatgttga aataactaaa tttctaagta
    41821 aagaaaacct gtgaatatgt tgattattat gtggataact attaattcga tatttattta
    41881 tatagtatga ataattgaat aatatatcta aaaaaagcca tcctattatt aagtaaactt
    41941 attgaaagga tggcttttta tgaatagaaa attgtgcaaa gcaataatac ctttatttat
    42001 ttctattttt tgcatgttaa taataagtat aaaagctgac caaggatatc aaaagaataa
    42061 tgacagagca attactgtca tgaataataa tgatactgaa gatgatgctg aaaaaaaaca
    42121 agaaatagac tatgaaaaaa catgttggaa tttgcaatct ttgtttaaaa gtgatgacca
    42181 atggaaaaaa gaacttaaga gttttgataa agatactaaa gagttgaaaa attacatagg
    42241 taaagtaaca aaatcgccaa cacatttgtc ttttgcctta aacattaaag aaaaacttga
    42301 tataagacta aataaacttt ctgcctatgt aaaattaaaa caagatacta ataaaaattc
    42361 gtataagtac ttagatatga atgatagtat gagtaaatcc tatgggaatt atatggggat
    42421 ttgttcagat ttagagttag aaatactaaa attatcaaac aaagattaca agaaaattat
    42481 ttcaaataaa aatataaata ggaaatatgg tgcatattta agagatataa gaaggaataa
    42541 agtacattat ttggatgata aatcagaaga tattctaagc aaagtatctt caataagtag
    42601 tcttccaagc caagtgtatg aattatttag aaatatggat agaaaaacta atttgacacc
    42661 agctcaatat gcgagtgaat tagagtcatc agatagagaa aatagaaaaa aagcttatca
    42721 agatgaactt ataacttaca atgataatat aaatacaatt gcaggtttaa ttactggtca
    42781 agttaaaaag aatatttttt attctcagga aagaggttat gagtcttctt tagaaatgta
    42841 cctggaatct gataatgtag atgaaaaagt atacaataat ttgataagga ctgtaaatag
    42901 taatatggac agtctacaca aatatataga tttaagaaaa aaagtattaa aattagataa
    42961 ggttcattca tatgatatgt ttgtgcctat ggttaaggct gttgataata atattaatta
    43021 tgaaaaggca caaagtatag tatattcagc gttgtcacct ttaggaaaag aatacaatga
    43081 tgtagtatat aaagctttca atgaaaaatg gatagatgtt tattcacatg aaaataaagt
    43141 aagtggtgga tactgcttat ctgtttatga aaatcatcca tatatacttt taaattataa
    43201 taattcatta ggttcagttt cgactttatc acatgagctt ggacatgctg tatatgaata
    43261 tttaagttct aagaatcaga attattttaa ttcaaatccg tctatattta ctcacgaagt
    43321 tgcatctaca acgaatgaag cattacttta cgagatgctt ataaaagaag ctaaaggcaa
    43381 tgatgaaaaa gcttattata taacacagta tttagacttg ataaaagata ctctttatac
    43441 acaaactatg tatgctgaat ttgaaaaaac tatacacgag ttagtagaaa aaggaaaaag
    43501 tgtaaacgca ttagtattaa atgatgtttg gggacaactt cttaaaaaat attatggaca
    43561 aagctatgaa ttagaccaat tatctaaggt cggatgggct agaatcccac atttttataa
    43621 tagtttctat gtctataaat atgctacagg ttgtagtgca ggagtaagtt ttgcccaaga
    43681 catcctaaag aatggttcag ataactatat gagctttctt aaaaagggaa gttcagatta
    43741 tccaataaac ttgttaaaat caagtggcat aaatttaact aatacaaaac ccatagaaaa
    43801 tacaatcaaa aaatttgata gtttagtagc agaacttgaa aagctattat aaacattatt
    43861 gtaatccaca tggtaaatat atcgaattaa tgataaaata tactaaaagt ttaaagtcct
    43921 atcaagatag gactttttat ttattaatta gtgggagatg attctatgca agttaaagtt
    43981 aaaaaggctg taataccagc agcaggttta ggaactagat ttttgccagc tacaaaggca
    44041 caaccaaaag agatgttacc aatagttgat aagccaactc tacaatacat aatagaagaa
    44101 gctgtggcct caggaataga ggaaatacta ataataacag gtagaaataa aaaatctatt
    44161 gaagaccact ttgataaatc tgtagaattg gaacttgatt tagagaaaaa aggtaagaaa
    44221 gaattgttgg aggtagtaca aaacatatct aacatgatta acatacatta tataagacaa
    44281 aaagagccaa aagggttggg agatgcaatt tattgtgcga ggcattttat aggtgatgag
    44341 ccgtttgcag taatgttagg ggatgatatt gtagataatg atgtaccttg tctaaaacaa
    44401 ctaacagatg catatgagga atatagaact acaatattgg gtgttcaaaa ggtaaatcaa
    44461 gaagatacta ataaatatgg gataatagaa gcaaaaaata tagaaggtag agtttacaag
    44521 gtaaaagaca tggtagaaaa gccagagtca ggaaaagctc cttcaaatat tgctatttta
    44581 ggtagatata taataactcc tgaaatcttt gatattctta aggatttacc accaggtaaa
    44641 ggtggagagg tacaacttac agatgcttta aaaatattat ctaagaaaga agctatgtat
    44701 gcatacaatt ttgaaggcaa gagatatgat gttggggaca agctaggatt tttagaagct
    44761 actgttgact ttgctctaaa gaaagaagac cttaaggaag attttataaa atatttgaaa
    44821 catgtatgta gtgaatttga taaaaataac aatgtgttat acaataatac agatgaaaaa
    44881 atagatttaa ttgaagtcaa tgaagctaga gaacttgaaa ttcaaaaata attaatgaaa
    44941 ggtgatgtat ggtggcaaaa tcaagtcaga aaaaaattaa tagaataata gctgttggga
    45001 ttgtgggaat cctcgtaata acgagtataa cagctgtttt gagtatgatt gcaactatgt
    45061 aataagataa atgacagcag ttgatataca taaatataaa aaccatatat aacttgatgt
    45121 agtcaaaaac ttacaatatt ttagttatat atggttttta gaatttataa atgtaaaaaa
    45181 aaatttttat aatagttgca tattttaaag gaaaatatta ttataagata taaatatagt
    45241 gataaattta taaaaacgtg ctaagattgg acttgaaata cagtaatata gtaggtaata
    45301 atgttttaaa ttttatttat tgaccataat atataaataa tgatattatt agacttaact
    45361 gtttaaataa aacttttctt tttcatgcct tcaatttcca aaataagtat gtatataaat
    45421 gaatatatag atgaggagga tttttttgat tttagatgat aaagtattag aaggattagg
    45481 tagtaagacc ttagttgagc tacgagagat agctaaagaa ttaaaaataa aatcaattac
    45541 tacatataag aaaaatgagt taattgagat tataaattcc aaaagtaaaa atgaggaaaa
    45601 agtaaacgaa gagactaaag aaaaaaatga agatattaaa gaagacataa agaatagaaa
    45661 agaaaatata gagcgtgaaa gtatagaaaa agaagttgaa tataagagcc ctaccaaatc
    45721 atacaacaag aaagaaatag atacccaaaa tatttcccaa aaccaagaac atagaaatca
    45781 aagaaatgac tataataaga caacgagtgt aaatgatagc aataagtcat caaatatatc
    45841 taatagaatg gtcagaaata ataataaaaa ctattatatg cctaaacaag ttgatgaatc
    45901 taagatagta gatgaattta acacatcaaa agaggatgaa gtagtaggtg tacttgaaat
    45961 tctgccagat ggatttggtt ttttaagagg ttcaaattat ttgtctactg aaggagatgt
    46021 ttacgtttca ccatctcaaa taagaagatt taatatgaaa acaggagaca agattaaggg
    46081 aataactaga catccaaaaa gtggagagaa atttagagca cttttatatg ttcaaaaaat
    46141 aaatgatgaa aaccctgaca cagctataca aagaaatgcg tttgagacat taacaccaat
    46201 atttccagaa gaacgactta cactggaaac taatagaaat gaaatagcta caagaattat
    46261 agatttaatt tcacctatag gaaaaggaca aagaggattg atagtagcac ctccaaaagc
    46321 tgggaaaaca gttcttttaa aaagcgttgc aaatagcata gctaaaaatc atccaaatgt
    46381 tgaacttata gtactcctta tagatgaaag accagaggaa gttactgata tgaaagaatc
    46441 tattgaagga gacgttatat attctacttt tgaccaagta tcaagtcatc atgtcaaggt
    46501 tgcagagatg gtgttaaata gagctcaaag attagttgaa catggtaaag atgtagtaat
    46561 tttactagat agtataacta gattggcaag ggcatataac cttacaatat ctcctacagg
    46621 tagaacttta tctggaggta ttgaccctgg tgcacttcat ggacctaaaa agttctttgg
    46681 tgcagcaagg aatattagac agggtggttc tttgacaata ttaggaacag cattggttga
    46741 aactggttct aggatggatg atgtaatatt tgaggaattt aaaggtacag gaaatatgga
    46801 gctacattta gatagaaaat tagcagaaaa aagaatattc cctgcaatag atatttacaa
    46861 gtctggtaca agaagagatg atttattgct tgatgatgaa gaaaagactg ctctttggag
    46921 attaagaaga gaaatgagta ataattcagt aatggaaatt acagataaag tcattgaact
    46981 tataaagaga actaaggaca ataaagagtt tgtaaaatca ataaagaatt tgtaaaatca
    47041 ataatacaaa tgcaaatatg gattatataa actaatagaa aaatcacttg aatggcataa
    47101 taagttgtgc tataataatg tagttgataa tgtaaaaaga aagttaatat aattaatttt
    47161 tttaaataaa gagaagaggt gactataatg caaaaggaaa tacaaccaaa atacaatcca
    47221 gttgaagtgc gttgtgcttg tgggaacaca tttgttgctg gttcaactaa ggacgaaata
    47281 aaagttgaaa tatgttcaga gtgccatcca ttctatacag gaaaacaaaa gaacatagaa
    47341 aaaggtggaa gaatcgacaa attcaagaaa agattcaaaa tggactaatt tttataaaag
    47401 tggagttgga ggaagccaac tcctatttta ttacaaaaaa acattcatat tctactttaa
    47461 attgtctttt tgtattataa taataaatat gtaaaattga gtattctaaa tacaataaaa
    47521 tgaggtgagt ggatggcaaa acaagctgta ggtgggcaag ctgttattga aggtgtaatg
    47581 atgcagtcaa aagacaaaag agcggtagct gttagaaaaa gtgacggaga aatagcacta
    47641 aaagaagata gaataaaaag ttgggtaaga gataagaata tagataagat accttttgtt
    47701 agaggttctt ttgttatgat agatacaatg attcaaggca taaaaagctt aaacttttct
    47761 tcagagtttt ttatggaaga agctgaagag gataaatttg atttatttat aaagaaaatt
    47821 tttaaagata aggcaaatga tataattata atattttcat tggtcatagc aatgttactt
    47881 tctgctggat tattcatttt tatacctaca ttggtaggag gagctttttc taaagtaatg
    47941 cctaatgatt ttatgcttaa tttaatagaa ggaatcataa ggatagctat attatttgca
    48001 tatatagtat tgatttcacg aagtaaggat atagaaagag tctttcaata tcatggagca
    48061 gaacataaat ctatatattg ctatgaaaat gatttagaac ttacagtaga gaatgctaga
    48121 aaatttaaaa ggttacatcc tagatgtggt acaaatttcc tatttatagt tatggctgta
    48181 tcaataatat tgtttgcatt ttttggttgg ccaaatccaa ttcttagaat atttatgaga
    48241 attatatgtg tgcctatagt agcaggttta tcttatgagg taattagagt tcttgggaaa
    48301 tatgataatg gatttactaa aattattgca tatccaggaa tgatgttgca atattttaca
    48361 acaaaagagc ctgatgatga gcaattagaa gttgctttgg aagcattgaa agcagtagta
    48421 gattagagaa aaggatattt gttatgacga taaaagatat aattattaaa tattcagata
    48481 aactaaaaga tataagtgat actccaagat tagatacaga gttattgtta caaaaaacat
    48541 tgggtgttga tagattatat attcacttaa atctgaataa agagctaact gaagaacaaa
    48601 aaacaaaatt tatgggtttt gcagaagaaa gattaaatgg aagaccaata gcatatatag
    48661 ttgaaaatag ggagtttatg ggattagatt tttttgttaa ggaaggcgta ttgattccaa
    48721 gaccagatac agaaacttta gtagaagaaa taattgaaat atgtagagaa aaaaaagatg
    48781 taagtatatt agatatagga actggttctg gagctattac aataagttta gcaaagtaca
    48841 ttgaaaattc taaaatcatg tcttttgata tatcagagac agcattagaa atagctaaaa
    48901 aaaatgctat tataaatgaa gttggtgaaa aaataaagta tattaattct gatttattta
    48961 cagcaataag tgatagtaac ataaaattcg atatcatagt ttcaaatcca ccatatatta
    49021 aaaaacaaga tatagaaact ctgcacaaac aagttaaaga ttatgaacca tataatgctc
    49081 ttgaaggtgg agaagatgga ttagactttt atagaagaat aacagaacaa ggtaagaaat
    49141 atttaaataa gtgtggaata ttagcatatg aagttggtca taatcaagct gaagatgtaa
    49201 taaatattat gaaaagtaat ggatataaaa aaatatacac aaagaaggat attcaaggca
    49261 ttgatagagt tgttataggt tacaatatat gatataatca acaattgata tgttacattt
    49321 aatcgaggtg aaagtatgtt aaaaaaatta gaggtattgg aagatacata taaagactta
    49381 agtgaaaaaa taggtgatcc ggatgtaata aatgatcaaa aagtttggca gaaatatata
    49441 aaagaacatg ctgacttgga acctattgtt atgaaataca gagagtataa aagcgtttta
    49501 gatagtataa aagaatcaaa agaaatttta caagaagagt cagatgaaga attaagagaa
    49561 ttagctaaaa tggagttagc tgagatggaa gaaaaagtag cacctctgga ggaagaaata
    49621 aaaatactat tattgcctaa agaccctaat gatgataaga acgttatagt tgaaattaga
    49681 ggtggagctg gaggagatga agcagctcta tttgctggtg atttatttag aatgtactca
    49741 aggtatgctg aaagaagaag atggaaaata gaattattga gtgcaagtga tactggagtt
    49801 ggtggatata aagaagtatc ttttatgata aaaggtaaag gtgcttattc aagattaaag
    49861 tatgaatctg gagttcatag agttcaaaga ataccatcaa ctgaatctgg tggaagaata
    49921 cacacatcaa cttctactgt tgcagtatta ccagaagttg aagatgtaga ggttgaaata
    49981 aatccaaatg atttaagaat agatgttttc cgttcttcag gtaatggagg acaaagtgtc
    50041 aatactactg actctgctgt tagggtaaca catataccta caggtgaagt agtatcttgt
    50101 caagatggaa aatcacaatt aaaaaataaa gagcaagctt taaaaatatt aaaagctaga
    50161 ctttacgata aagcattggc agaacaacac aaagatatag cagcagaaag aaaaagccaa
    50221 gtcggaacag gagacaggtc agaaagaata agaacatata atttccctca aggtagaata
    50281 agtgaccata gaataaattt aactttatat aagttagatg cattcttaga tggagatata
    50341 gatgaaatga tagatgcttt aattactgtt gaccaaacag aaaaaatgac tgcaatataa
    50401 gtaatgtttt aaagaagctg tttcttccta gaaattattt tctattttga aacagctatt
    50461 ttgcatttta ggtcatgtta tcatattcta ttgtatataa ttgttatata caagtatagg
    50521 agggttatat gatactaaaa gtaacaataa ttgggctttt ggcaggtgtt atagggacag
    50581 gattaggagg agtaatatca gccattttta aaagagaggt cgataaatat ctaagttttt
    50641 ttatgggatt atctggtgga ataatgttgg ctgtagtggt gtttgattta atgaaagaat
    50701 ccatggacaa aatggggata atcaatacag ttatatttac gtttgttgga gctctcataa
    50761 ctatgtacat aaaaacaaaa ttagatgtgt ctggaaatat ggcatctgga tatcttatat
    50821 tcataagtat attactacat aatttaccag aaggactagc cattggttct tcttttatgt
    50881 caactgaaag tttaggtatt acacttgcta tagttatagg tctacataat ataccagaag
    50941 ggttagctat ggcattaggc ttagtttgca ataaaatgaa actaagtaaa gttattttgt
    51001 ttactgtaat agctggatta cctatgggtc taggaagttt tttaggtgtt tattttggag
    51061 gagtatttac ttctcttata ggagtatttc ttgctacagc aggaggtact atgatgtatg
    51121 ttgtattgga agaaattttt ccacattcaa aaagtgtgta ttgtataatt ggtttcttgt
    51181 taggtacaat gattgtaaat tatatataaa attaaaatga aagcaggcat acaaaagatg
    51241 aaaactatta taagtaatat tgatataaat aatattgatt atgaggaaat aaaaatacaa
    51301 gcaaaacttc ttagagaagg taaaacggtt atttttccaa ctgagacagt atatgggttg
    51361 ggtgcaaatg cattagatga aaatgcagta aagaagattt atgaagctaa aggaagacct
    51421 agtgataatc cattgatagt ccatatatat gaaaaagaag aagtatatga cttagccaaa
    51481 gatataagtg ataaagctaa acttgttata gaaaagcttt ggccaggacc tataacaatt
    51541 atattaaata aaaaagatat aattccatac aaaactagtg gagggcttga tacagttgca
    51601 ataaggatgc cttctaatgt tattgccagg gcaataataa aagaagctgg tatacctatt
    51661 gcagctcctt ctgcaaatat ctcaggtcgc ccatctccaa caaaagcaaa acatgtatat
    51721 gaagagatga atggaagagt ggatggaata gttttaggag gagacagtaa ttttggattg
    51781 gagtcaactg tattggattt aacagaagag actcctatga tactgagacc aggaagtata
    51841 actaaagaag ttttagagga acttcttgga gaagtaaagc ttgacccatc attaagtaaa
    51901 aaagaagata atcaaaaagc taaagctcct ggaatgaaat ataaacatta ttctccaaat
    51961 gcagatgtat atataatatc tggaaaaaga gaaaatgtag caacaaagat taatgatatg
    52021 ataaaatcta atagagacaa aggtctaaag acaggtgtaa tgtgtattag tagaaaccga
    52081 gagttttatg atggagaagt aatcgattta ggaaactctc ttgaagaagt tgcaagtaat
    52141 ctgtttgatg ctcttataga aatggacaaa aagggtgttg atgtcatata ttcagaagag
    52201 ttccctaaga ctggagttgg acaagcaatt atgaatagac tactgaagtc agcaggatac
    52261 aaagtgataa aggcttaaga gggagttgta aaatatgaat atacttattg tgtgtacagg
    52321 taatacttgt agaagcccta tggctgaggc tatactaagg aaagcaatta aagaatctgg
    52381 aagaagtatt gaagaatatt ctatatcatc agcaggtata tctacagcaa atggaatggg
    52441 tgcttctgaa aattctatag aagtactaaa agaaatcggt atagacttat caaatcatag
    52501 aagtaaagta attactaaaa aattaattga tgaatcagat ataatactaa caatgactaa
    52561 atctcataaa gaaattttag tacaagcagt tccaaaatgc aaagaaaaag tgtatacttt
    52621 taaagggttt gcaaataaaa atgaggaaga tatatctgac ccatttggtg gaaatttgga
    52681 tatttataga agcactatga gagaaataat gtattctgtc aacgaaatag taaaaaaaat
    52741 atgaaacgga ggataaacaa atgaagatag gattaggctg tgaccatgga ggatataatt
    52801 taaaaaaaga aataataagc tatttagaaa gtaaaggtat agagtgtgta gattatggaa
    52861 ctaataatgc tacagattct gttgattatc ctgtttatgg agaaatagtt gcaaattcag
    52921 ttataaataa agaagtagat tatggaatat tatgttgtgg tacagggata ggaatatctt
    52981 tagctgctaa taaggttcct gggataagat gtgcagttgt atcagatgta ttttctgcaa
    53041 aaatgtcaaa agcacataat gatgcaaata tgttatcatt aggcgaaaga gttttaggaa
    53101 aaggtctagc acttgagata gttgaagcat ggattaatac ggattttgaa ggtgatagac
    53161 atgctagaag agtaaacatg attaaatcaa tagaagaaaa acataataaa taataaaaat
    53221 tacagtttta tcaggaggaa ttatcaaaat gagcaaagtg gtagagacaa accatccatt
    53281 aatacaacat aaattaactt taatgagaga taaaaatact ggttctaaag actttagaga
    53341 gcttttaact gaaattgcaa tgttaatggg gtatgaaata acaaaagata taccattaaa
    53401 agatgtagaa atagaaactc ctattcaaaa aacatcatct aaagtagttg caggtaaaaa
    53461 attagcaata attccaattt taagagcagg tcttggaatg gtagatggat tagtgagctt
    53521 aatgccagca gcaaaagttg gacatgtagg actatataga gacccagaaa cattaaaacc
    53581 agttgaatat tattgtaagc ttcctcaaga cataggagaa agagatataa tagtagttga
    53641 ccctatgctt gcaacagggg gttctgctgt agcagctata gatttactta agtctaaagg
    53701 agctaaaagt ataaaattag ctaatttagt agctgcacca gaaggtatag cagaagtgca
    53761 aaaatatcat gatgatgttg atatatatgt tgcatcagtt gacgaaagat taaatgaaca
    53821 tggatatata attcctggtc ttggtgatgc tggagataga ttgtttggaa ctaaataaaa
    53881 ataaataact ataaatattt attagggact ttcttatata gtgtatataa ttttatgtaa
    53941 gaaggttctt ttttatatat tttttctcaa aataggaatt attgaattga taaatttctc
    54001 gaattgagaa taaaaagttt ttaccaatta aaaaagattg cttattttac aaggttttat
    54061 agaatagtag tctattttaa tgtggcatgt tagttgctta cgattaagat tataggtatt
    54121 aagtgtatta gttaaattga gaattgaata ttaataatat tattgggagt ggtaatacat
    54181 gaagaattgt atagtagtaa gaggggctgg agatttagca acagcagtta ttcataaatt
    54241 acacaacagt ggttttaaag tagttgcgtt agaaatagaa gagccattag ctataagaag
    54301 gcaaatttct ttttgtgagg ctgtttatga aaaaaaagta actgttgaag gtgtagaatg
    54361 tatattttgc aatactgaaa aagaaataga agaagtttta tctcaacata aagtagcctt
    54421 gattatagac ccaaaaggag aaaaattatc taaattaaat ccaatcgttt tggttgatgg
    54481 aatactcgct aagaaaaact taggtacaac taaagatatg gctccactta caatagcttt
    54541 aggtcctgga ttttgtgcag gagaagatgt agatgttgtt atagagacaa tgagaggtca
    54601 taatctagga agaataataa ctaatggtag agcagaaaaa aatacaggag taccaggagc
    54661 aataaaaggt ttttctaaag agagagttat gcattcacag tatgatggca taatggaaaa
    54721 tgtatgtaag ataggtgata tagttgaaaa gggagaccta ctttgctata ttaataatta
    54781 tgaaaaaaaa catgaagtta gagcaacaat atctggagtc cttagaggtt tattaaagga
    54841 tggagcaaag gtaaacaaaa atttaaaaat actagacata gaccctagaa aagaagaagt
    54901 taagaatgct tttacaatat cagataaagc tagatgtata gctggagggg ttttagaagc
    54961 catactaaac aatgggattt atccccaaaa ttaatttata ttagataaag ctattaatat
    55021 tttattaaga tgatataata aatttaatag attaaagaga gcatatgacg ggtatttaaa
    55081 atttaaatgg taaaaatgct ctttttttat tggacattca attaacatgt gtatataata
    55141 taaaaataag tatttattta ggttgaaatc taataaatat ataataccag tacataagca
    55201 aagaggagaa aagtaaatta tgagaccatc atgggatgaa tacttcatgg aaattgcaga
    55261 agtagtgaaa aagcgttcaa cttgtattag aagacaagta ggagctgtaa ttgtaagaga
    55321 caaacaaata ttaacaactg gatataatgg ttctccgaga aatcttgaac actgtgaaaa
    55381 tattggatgt aagaggcagg aattaaatat tccctcagga gaaaggcatg aattatgcag
    55441 agcattacat gctgaacaaa atgcgataat acaagctgct cataatggta ttagtgtaga
    55501 tggggcaact ttgtatgtta ctacaagacc ttgtgttttg tgtgcaaaaa tgtgcataaa
    55561 tgcaggtata ttgaaaattg tttatgaagg tgattatcca gatgatatgt ctacagaatt
    55621 attaaaagaa gcaggaatag aactcataaa attttagtgg ataaaattta tatgacaata
    55681 aatgtcaaat tatgacactg aaatacaatg gtatcaaatg attaaaagaa agtatttgaa
    55741 aaatcatcag attagtagta agatatattt tataattaaa taaatttatg agatagatta
    55801 attacatgtt atttatttta taataatgag ggatactatt tatgagtaaa agaaatacac
    55861 atacagaaat tgcaaagttg tttagtttaa taagccaaat aggattgatg attgtaatat
    55921 caatattagg atgtactttc ttaggaaaat ttttagatag taaattgaat actactccag
    55981 ttctgacaat aatattttta ttgttgggtg ttggaggtgc ttttagtggt gtttataaaa
    56041 ctctaatagt atatacacga aggaagtgat atattggata taaaattact taaccaggta
    56101 aggtcagtaa caaaaggtat agtaatattt gatgtagttt taattgtgct aatgttgata
    56161 acatcaacat taagtaaacc agtattatta ggacttatat ttggctctat aatagctatg
    56221 ttaaatttta gattattggc gatatcactg gaaaaatcag tgacatttcc tgctgggaag
    56281 gcacaagctt atgcatcagc acaatatgct gttagattat ttataatggc agttgttcta
    56341 attgcatcag ctacagtacc acatttaaat ataatcggaa cagcattggg attattgagc
    56401 acaaagtttg tcatattatc aaaaaacttt ataaataaat taaagaggaa ggaggcataa
    56461 gtatgaatca agcgcattat tatctcatag gaaactttaa gattagtgaa acaatactag
    56521 taagttgggg aataatggcc tttttagcga tagcatctta ttttttgact aaaaacttaa
    56581 aaaaagttcc aactagtaaa gttcaaatat tcttagaatt tgcagtaggt ggtcttgcta
    56641 aaatggttac agatgttatg ggtaaaaata cagtaaaaag aatgccatat atgataccat
    56701 acataggaag cttgttttta tttttcgcat gttcaaattt aagcccatta cttggattaa
    56761 ggtcaccaac aaccgactta gatacaactg ctgcatgggc aatgattaca ttttttatga
    56821 tttattttgc aggagttaaa tttaaggggt tatcatactt taaagaattg gctcaaccac
    56881 ttgccataat gttaccaatc aatattatag gtgagttagc tagaccaata tcattaagtt
    56941 tccgtccatt tgggaatatt ttaggtggaa caataataat gggtctgttt tatcaacttt
    57001 taggttttat ttctagttta ataccaggat tatcaatacc aattggagaa atattaatac
    57061 cagtcccatt acacttgtac tttgacttat ttgcaggatt gttacaagca tatatattta
    57121 taatgttaac aatggtattt atatcaagtg caggagagtt agattaagaa atcaacttat
    57181 attaagataa aaataaaata atttaaaaaa ttaaagtttt aaggaggaaa taattatgga
    57241 aacagcaata gtagcggcag catcagcaat aggagcaggg atagcagttg caacaggtat
    57301 aggtgcaggt ataggtcaag gtatagcagc agctaaagct gcagaagcag taggaaacca
    57361 gccagaagct aaaggtgata taacatcaac tttattacta ggagttgcta tagcagagtc
    57421 ttcagcaata tatggtttgg ttatatcaat aatacttcta ttcgtaaatc cattctttaa
    57481 atacttagga atgtagattt agtccttaaa tattttgaga taggtttata ggtaaatcat
    57541 ctcaaactta taatcatgag atgaacttta tcatctcgtg attataagta gttatgattt
    57601 atggaaagga ggataattat ggaaaaagca ctagtaggta taacgtggga atttgtgttc
    57661 caaattgtta atacttttat aatattttta cttctgagaa agcttttatt caagccagta
    57721 ttaaatataa ttgaatcaag agaaaatgat attaaaagtg atttggcaga aggcgaaaaa
    57781 gcaaaaaatg aaggtttagc tctgaaaaaa gaatatgagt caaaaatcaa ttttgccaaa
    57841 gatgaaggtc aagaaatcat aaaacaagca actataagag cagagcagaa atcagatgat
    57901 atagtaaata ctgcaaagaa agatgctctt gatataaaag aaaaagcaaa caaagatata
    57961 gaacaagaaa gacaaaaggt tataaatgaa atcaaaaatg atatatctaa catagcacta
    58021 cttgcagcat ctaaagttat tgaaaaagat ttagataaat ctaaacatga agaattgata
    58081 gaaaacttta taaaagaggt aggggaagct aaatgataaa tgtaatagca aatagatatg
    58141 ccgaagccct atttcaatta ggagaagaag aaaattcaac tgatgtctta ttcaaagaac
    58201 tagaaaaagt agtagatatg atgactaaag ttagtaaaga tttctacaaa gttttaaaat
    58261 caccattagt ttcaaaatca gaaaagaaaa acttggtaga aattattttt agtaaagaag
    58321 taagttctaa cataaaaaac tttttaaaag ttttagtaga taaagataga atatcatatt
    58381 tagaagatat cgaattagct tataaagaat tattaaataa gaaaaacaat gtaattgatg
    58441 gagtagcaat aagtgctatt ccaatgagtg aaacagatat taaagaactt gaagtcaaac
    58501 tttcaaataa atacaacaaa aatgttacta tagaaaatgt tgttgacaag actatactag
    58561 ggggagttct agttagaatt ggaaatgaac aaatagatgg cactgtgaaa actcgtcttg
    58621 ataagatgaa agaaaagcta tctgaagtaa tatcttaaga atggcggtga acccatgaac
    58681 ttaaaacctg aagaaataag ttctataatt aaacagcaaa taaaaaatta tgagaataaa
    58741 gttgagttga cagatacagg tagtgtttta acagttgggg atggtatagc gagtgtatat
    58801 ggattagaaa aagctatgtc tggtgagttg ctagagttcc caggtgaaat atatggtatg
    58861 gcacttaact tggaagaaga agtagttgga gctgttatcc ttggagatga cagtgaaata
    58921 aaagaaggcg atatagttaa gagaactgga agaatagttg aagttccagt tggagaagct
    58981 ttaataggta gagttgtaaa ctcactaggt cagccaatag atggaaaagg acctatagcg
    59041 tacacaaaaa ctagaccagt tgaatctgag gcacctggta taatagatag aaggtctgtt
    59101 tatgaaccat tacaaacagg aatcaaatca atagattcaa tgataccaat tggtagaggt
    59161 caaagagagt taataatagg tgacagacaa actggtaaaa catctatagt tattgacact
    59221 atactaaatc aaaaaggaaa agatgttatc tgtatatatg tagcaattgg acaaaaacgt
    59281 tctactatag ctcaattagt tagctcttta gaaaaaggtg gagctttgga ttatacaata
    59341 gtagtttcag caacagcatc tgaatcagca ccacttcaat atatagcacc atatgctgga
    59401 gcagctatgg gtgaagaatt tatgtacaat gggaaacatg tacttatagt gtatgatgat
    59461 ttaagtaaac aagctgttgc atatagagaa atgtcattgt tacttagaag accaccagga
    59521 cgtgaggcat atcctggaga tgtattctat ttacattcaa gattacttga aagagcagct
    59581 aagttatctg atgaattggg tggaggttca atgactgcct tgcctataat agaaactcaa
    59641 gctggtgacg tttctgcata tataccaaca aatgttatat ctataacaga tggtcaaata
    59701 tacttacaac cagagttatt ctattcaggt gtaagaccag cagttgaccc tggtatatca
    59761 gtatcaaggg ttggtggttc tgcgcaaatt aaagcaatga aaaaagttgc aggaacatta
    59821 aaacttgcat attcacaata tagagaactt gcagcattct cacagtttgg ttctgactta
    59881 gatgaagata ctaaaaagag acttgctcaa ggtgaaagaa tcgttgagat attaaaacaa
    59941 ggtgagcatc aaccaataaa agttgaaaat caagttatga taatatatgc tgttataaat
    60001 aatcatttag aggatattcc gatagataat atagctagat ttgaatcaga attgtatgca
    60061 tttgtagata ataattatcc ggaaatatca agaaaaatat taggtggaga agacttcact
    60121 catgatttaa cagatgcaat aaatgaattt aaagaaaagt ttgttgttga agtataatcc
    60181 tttttaaaaa aggaggtaaa ttaattggca ggagctggaa tcaaagcaat caaaacacgt
    60241 atgggaactg ttgaaagtac aaagcaaata actaaagcaa tggaattagt tgcatcttct
    60301 aagcttagaa aagcaaaaga aaaggctgaa gattcaagag aatattttaa tactttatac
    60361 aatacagtaa aagatattgc ccaaacaaca cgtggaatta gaaatgaatt tttaaaagaa
    60421 agagaagtaa aaaataaatg ctatgtagta gtagctggag acagaggact tgcaggtgga
    60481 tacaattcta atgtattaaa ggaatctgtc tctcatatga aaggtaaaaa ggaaacagtg
    60541 attactgttg gaaagaaagc acatgaattt ttttctaaaa gaaactacaa tatagcaaaa
    60601 gatataaata gtgttgaagc ttgtggatat gaagatgcga cagaaatttc acaaactatt
    60661 attgacctgt ataaaaaagg tgaagtagat gaagtttata ttgcttatac aaaatttaaa
    60721 tctgctttat cacaagaagt tgaaattttg aaattgcttc cattagtctt tgaaaaagat
    60781 acagacgaac aaggaatggc agtaaaagaa gaaaaacaaa atagatcaaa agtacaatat
    60841 ataccatcag cagaagcagt tttaagttat attgtacctc agtttatatc aggtacagta
    60901 tatggtggaa ttttggaatc ttttgcatca gagcaaggtg ctagaagaac tgcaatggaa
    60961 tcagccacag acaatgcgaa tgaaatgcta tctaatttag agttaagtta taacagagca
    61021 agacagtcag ctgtaactca agaaataaca gagatagttg gtggagttga agctctaaaa
    61081 tagggaggtt aggaaatggc aaatgtaggt aaagtagtac agattgtagg agctgtacta
    61141 gatgttaagt ttgatagtga acaaagccta cctaacttat taaatgcgtt agtaataaag
    61201 ttaggagata aagaaatagt tgcagaagtt gctcaacata taggtgatga tactgtaaga
    61261 tgtatagcta tgagtgcaac agacggactt gtaagaggta tggaagttgt agatacagga
    61321 gggccaataa gtgttcctgt tggagatgaa actttaggta gaatattcaa tgttcttgga
    61381 aaaccagttg atgggaaacc agctccaaaa tctgcaccta aattacctat acatagacca
    61441 gcaccggcgt atgatgaact agaaactaca gcagagatac tagaaactgg tattaaggta
    61501 gttgacttac tagctccata tctaaaaggt ggaaaaatag gtctatttgg aggagcagga
    61561 gtaggaaaga cagttctaat acaagagctt ataaacaata ttgctaagca acatggtggt
    61621 atttctgtat tttcaggagt aggagaaaga acaagagaag gtaacgacct ttatggcgaa
    61681 atgagtgagt ctggagttat aaataaaaca gctctagtat ttggtcaaat gaatgaacca
    61741 cctggagcga gaatgagagt tgctttaact ggacttacaa tggcagaaca ttttagagat
    61801 gagcaaggac aagacgtttt acttttcgtt gataatatat tccgtttcac acaagctggt
    61861 tcagaagttt cagcacttct aggacgtatg ccatcagctg ttggttatca gccaacatta
    61921 gctactgaaa tgggtgcact tcaagagaga ataacatcaa ctaagaaagg ttcaataaca
    61981 tctgttcaag cagtatatgt accagcagat gacttaactg acccagcacc agctacaaca
    62041 ttctctcact tagatgcaaa aacagtttta tcaagacaaa tatcatcact gggtatatat
    62101 ccagcagttg accctcttga atctacatca aggattcttg accctagtat agtaggaaaa
    62161 gaacattatg aagtagctag aggtgtacaa tcaatacttc aaagatataa agaacttcaa
    62221 gatataattg ctatacttgg tatggatgaa ttatctgatg aagataagtt aatagttgca
    62281 cgtgctagaa agatacaaag attcttatct caatcattca cagttgctga gcaatttaca
    62341 ggaaatccag gtcaatatgt tcctgttaaa gagactgtaa gaggatttaa agaaattctt
    62401 gaagggaaac atgatgactt accagagtca gcattcttat ttgtaggaac aatagaagac
    62461 gctgttagaa aagcgaaggg aagtatgtag tcatggccag tgaattttca ttgaaagttg
    62521 taactccaag taatactttt tatgatggag atgttgaaat gatagtagtt agaactacaa
    62581 aaggagacaa aggggtgttg aaaggtcata taccatttgt ttcaggtata cctgtaggaa
    62641 ctttaaagat aaaaaaagag ggaagtttta aagaggcgaa aatagcagaa ggattcatta
    62701 atgtaactca agataaaact gtcatattga cagaatctgc tgagtggatt taataaatta
    62761 atttagagta taaaaagtca ttttgaccaa aaaaactttt gggtaaaatg gcttttttgt
    62821 gttttggtgt taataaaata acgataaaat tgtaagtttt tagtgataat aaattcacaa
    62881 aatgtttaaa tttactgcca aattatgttg taattaataa gataaagaag atttagatag
    62941 cttttatgta agtgaaaaag tcttcatata agaaatacat ataaaatttt tataaaattt
    63001 aaaaaatttg cttag
//
LOCUS       DAEORZ010000003        53772 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEORZ010000003 DAEORZ010000000
VERSION     DAEORZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.003"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            95..2323
                     /locus_tag="KRH39_000134"
     CDS             95..2323
                     /locus_tag="KRH39_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBG8510068.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLKPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSVSVLNNVLSLSNGIGRINLNILNNFDSKISRPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVNGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(2560..2859)
                     /locus_tag="KRH39_000135"
     CDS             complement(2560..2859)
                     /locus_tag="KRH39_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBG8510069.1"
                     /translation="MYKNINKNQLKEMMKNEKDILLLDVRTKDEFREYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            3067..4098
                     /locus_tag="KRH39_000136"
                     /pseudo
     CDS             3067..4098
                     /locus_tag="KRH39_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
     gene            4812..5057
                     /locus_tag="KRH39_000137"
     CDS             4812..5057
                     /locus_tag="KRH39_000137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510070.1"
                     /translation="MKRIKKVFRDLVENYKEEKFEKQEAIEMQEAIEAIRQEIGRHDP
                     TINTPKDSAGNVGDEPPFISNPLVANQPIKNPKYKGN"
     gene            5498..7339
                     /gene="ltrA"
                     /locus_tag="KRH39_000138"
     CDS             5498..7339
                     /gene="ltrA"
                     /locus_tag="KRH39_000138"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBG8510071.1"
                     /translation="MYNTDTKSIIKQDLQPLKKDKLRYNEYYNLQNTFDNLYKQSLEG
                     SKFTKLFNLISSEENILLAYRNVKTNTGSKTAGTNRHNINYWKTKDTKIFVQYIQNRL
                     MNYKPQKVRRVEIPKPNGKIRPLGIPCIEDRIIQQCIKQVLEPICEAKFHPHSYGFRP
                     NRGTEHALAYVVKKINFEHMYTVIDIDIKSFFDNVKHSKLLKQLWSIGIHDKKLLSII
                     SSMLKAEIDKVGIPNKGVPQGGILSPLLSNIVLNELDWWISNQWQTHKTHYQYSTTSH
                     LYRALRKTNLKEMYIVRYADDFKIMCKNVSEANRIFIATQKWLKERLGLEISQEKSKI
                     TDVRYSNSEFLGFKIKAYKKRNKYIVYTKMTDKAKEQVSHNIKEQLIAIQHFPTTKNI
                     NMLNRIIAGSHNYYKIVANVYQDFKNIKYNLSFSLYNRLKNLRTSTGNLTHEYESKYS
                     KYNLKKQYILNEIMYPLTGIKTTPPFLFNQTVSNYTKEGRKIIHTRLGCIDNNILNYI
                     AHNPILNKSVEYNDNRLSKYSAQKGTCIISGYPLVENMEAHHITPVNKGGDDKFRNLI
                     LVIPEIHKLIHATNIEHIQYYIKELNLDNQEIKKLNKYRKKIGNEII"
     gene            7441..9453
                     /locus_tag="KRH39_000139"
                     /pseudo
     CDS             7441..9453
                     /locus_tag="KRH39_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
     gene            complement(9615..10889)
                     /locus_tag="KRH39_000140"
     CDS             complement(9615..10889)
                     /locus_tag="KRH39_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG8510072.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NENEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCINACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNDALASHRAMGAIL
                     SAILKLEAAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(11111..13672)
                     /locus_tag="KRH39_000141"
     CDS             complement(11111..13672)
                     /locus_tag="KRH39_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8510073.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQIRETIKNDGSFHVTFYDVNRKNVEYVTKSAEVEKHAFTKQLGYSKLENSENGIVS
                     IKQYDKNAINGYKISIKEGRFPAKEGEIVLSDNIINLIDDNLKIGDKITLKVGDMFDS
                     SKKKIDSMVFHEGDYIANEKDRTFEVVGIIKKPGFERYNGIATAITYFNPMDNYNDTI
                     NVSVALRNPKDVYEISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYANINNSI
                     KSIIIAVTILVIICTIATVYNSFSISITERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKHFTESVVTQMSLKIVYNPIIIIVSVIIVLFTIF
                     ISILLPAISASNISPLNVIKNNGEYKVGKVKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     TVTLFSLMVSIIIFISFSGFTLLLLKGEEIRNSQRNYDLYLTAKGTAKSIDDTISKLE
                     AISGIKNFELATGQYMSIRVSENKINKSKEELIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKTNNLIQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKIKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFSFYDESASNESEKQDLKVIKIFVYGFIAVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSIFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            complement(13673..14350)
                     /locus_tag="KRH39_000142"
     CDS             complement(13673..14350)
                     /locus_tag="KRH39_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510074.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDGEYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVI"
     gene            complement(14430..15674)
                     /locus_tag="KRH39_000143"
     CDS             complement(14430..15674)
                     /locus_tag="KRH39_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8510075.1"
                     /translation="MYLYNPEVKKFLSKYVTLMFIVIIISIGFSVINVSLTKDMVVRN
                     NQAIIGTLSSKYPNLESDIVDIITQGKSTGNIDYGQKILSKYNYDKSIKINSEPIISK
                     LVLETIKINIILVCIIFILILVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAMQ
                     SMKIPMEIKNQNLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            complement(15661..16365)
                     /locus_tag="KRH39_000144"
     CDS             complement(15661..16365)
                     /locus_tag="KRH39_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8510076.1"
                     /translation="MLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSGKEMISSN
                     EYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGGDDYITKP
                     FRIRELISRIKAVLRRKVNTVEESKKILKFGDLSIYTLEARVYKNDKEIFLTSVEYKL
                     LLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCEPIYIETV
                     RGIGYKWIGSENSVSI"
     gene            complement(16650..17096)
                     /locus_tag="KRH39_000145"
     CDS             complement(16650..17096)
                     /locus_tag="KRH39_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510077.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            17288..17677
                     /locus_tag="KRH39_000146"
     CDS             17288..17677
                     /locus_tag="KRH39_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8510078.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLALELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            17670..18761
                     /locus_tag="KRH39_000147"
     CDS             17670..18761
                     /locus_tag="KRH39_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBG8510079.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENTKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            18881..19357
                     /locus_tag="KRH39_000148"
     CDS             18881..19357
                     /locus_tag="KRH39_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG8510080.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            19743..20210
                     /locus_tag="KRH39_000149"
     CDS             19743..20210
                     /locus_tag="KRH39_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG8510081.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(20270..20842)
                     /locus_tag="KRH39_000150"
     CDS             complement(20270..20842)
                     /locus_tag="KRH39_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG8510082.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKMGIRDYLKRLNI"
     gene            complement(20905..22002)
                     /locus_tag="KRH39_000151"
     CDS             complement(20905..22002)
                     /locus_tag="KRH39_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG8510083.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            complement(22016..22705)
                     /locus_tag="KRH39_000152"
     CDS             complement(22016..22705)
                     /locus_tag="KRH39_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG8510084.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            complement(22870..24036)
                     /gene="rpoD"
                     /locus_tag="KRH39_000153"
     CDS             complement(22870..24036)
                     /gene="rpoD"
                     /locus_tag="KRH39_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBG8510085.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            complement(24054..25844)
                     /locus_tag="KRH39_000154"
     CDS             complement(24054..25844)
                     /locus_tag="KRH39_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBG8510086.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            complement(26099..26884)
                     /gene="aroF"
                     /locus_tag="KRH39_000155"
     CDS             complement(26099..26884)
                     /gene="aroF"
                     /locus_tag="KRH39_000155"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG8510087.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            complement(26865..27371)
                     /gene="folK"
                     /locus_tag="KRH39_000156"
     CDS             complement(26865..27371)
                     /gene="folK"
                     /locus_tag="KRH39_000156"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBG8510088.1"
                     /translation="MNKVYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVVSTDEKLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            complement(27364..27729)
                     /gene="folB"
                     /locus_tag="KRH39_000157"
     CDS             complement(27364..27729)
                     /gene="folB"
                     /locus_tag="KRH39_000157"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBG8510089.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            complement(27740..28531)
                     /gene="folP"
                     /locus_tag="KRH39_000158"
     CDS             complement(27740..28531)
                     /gene="folP"
                     /locus_tag="KRH39_000158"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG8510090.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSEEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            complement(28597..29169)
                     /gene="folE"
                     /locus_tag="KRH39_000159"
     CDS             complement(28597..29169)
                     /gene="folE"
                     /locus_tag="KRH39_000159"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBG8510091.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            complement(29195..29896)
                     /locus_tag="KRH39_000160"
     CDS             complement(29195..29896)
                     /locus_tag="KRH39_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510092.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRNIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            complement(29930..30673)
                     /locus_tag="KRH39_000161"
     CDS             complement(29930..30673)
                     /locus_tag="KRH39_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBG8510093.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            complement(30663..32012)
                     /gene="pabB"
                     /locus_tag="KRH39_000162"
     CDS             complement(30663..32012)
                     /gene="pabB"
                     /locus_tag="KRH39_000162"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBG8510094.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            complement(32014..32598)
                     /locus_tag="KRH39_000163"
     CDS             complement(32014..32598)
                     /locus_tag="KRH39_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBG8510095.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIIDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            complement(32622..33341)
                     /locus_tag="KRH39_000164"
     CDS             complement(32622..33341)
                     /locus_tag="KRH39_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG8510096.1"
                     /translation="MTSWKRKTVYKCLIAVVLFCGIVLISNFSKVSALMLDTNENVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            complement(33369..34433)
                     /locus_tag="KRH39_000165"
     CDS             complement(33369..34433)
                     /locus_tag="KRH39_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG8510097.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DTSEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQLNAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            complement(34577..35386)
                     /locus_tag="KRH39_000166"
     CDS             complement(34577..35386)
                     /locus_tag="KRH39_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510098.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     AMEYIGEFKEDKKNGKGNFVFSNGNEYIGYFEEDMMSGIGNMLYNSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            complement(35628..36494)
                     /locus_tag="KRH39_000167"
     CDS             complement(35628..36494)
                     /locus_tag="KRH39_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG8510099.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            complement(37014..37703)
                     /locus_tag="KRH39_000168"
     CDS             complement(37014..37703)
                     /locus_tag="KRH39_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBG8510100.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            complement(37696..38067)
                     /locus_tag="KRH39_000169"
     CDS             complement(37696..38067)
                     /locus_tag="KRH39_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBG8510101.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            complement(38249..39595)
                     /locus_tag="KRH39_000170"
     CDS             complement(38249..39595)
                     /locus_tag="KRH39_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG8510102.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            complement(39777..40370)
                     /locus_tag="KRH39_000171"
     CDS             complement(39777..40370)
                     /locus_tag="KRH39_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBG8510103.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(40435..41904)
                     /locus_tag="KRH39_000172"
     CDS             complement(40435..41904)
                     /locus_tag="KRH39_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG8510104.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYSGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(42019..43410)
                     /locus_tag="KRH39_000173"
     CDS             complement(42019..43410)
                     /locus_tag="KRH39_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBG8510105.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(43576..43824)
                     /locus_tag="KRH39_000174"
     CDS             complement(43576..43824)
                     /locus_tag="KRH39_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510106.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            44090..46219
                     /locus_tag="KRH39_000175"
     CDS             44090..46219
                     /locus_tag="KRH39_000175"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBG8510107.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLARPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            complement(46478..47077)
                     /locus_tag="KRH39_000176"
     CDS             complement(46478..47077)
                     /locus_tag="KRH39_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBG8510108.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIYGSEKLSFQEDKEFIEAYVDGSYEHSIKMYGSGVVILKNNEVIKTYSEKGKEE
                     TLVSMRNVAGEIEASKIAMQYCIDNNIQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(47095..50214)
                     /locus_tag="KRH39_000177"
     CDS             complement(47095..50214)
                     /locus_tag="KRH39_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG8510109.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIHTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     NFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKGGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            50435..51259
                     /gene="pdaA"
                     /locus_tag="KRH39_000178"
     CDS             50435..51259
                     /gene="pdaA"
                     /locus_tag="KRH39_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG8510110.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(51356..52141)
                     /locus_tag="KRH39_000179"
     CDS             complement(51356..52141)
                     /locus_tag="KRH39_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8510111.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            52606..52971
                     /locus_tag="KRH39_000180"
     CDS             52606..52971
                     /locus_tag="KRH39_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8510112.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            53049..53603
                     /locus_tag="KRH39_000181"
     CDS             53049..53603
                     /locus_tag="KRH39_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG8510113.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
BASE COUNT        18020 a         8783 c         5981 g        20988 t
ORIGIN      
        1 gacaaaattt taactttact atttgtgtaa aaagtaatat aatttaaaat tgattataaa
       61 tctaactttt ttctttatca aaatatttta ggaggtgaga catttgaaaa aaactaatag
      121 tttaaaagaa attttaaact ggaataatat caagaattta aaaccttcaa agggtttaaa
      181 aaatatatta gaaattttta aaagtgacct aaaagatata cgtggtaatt atgcagtact
      241 tgttatagtt ttaggtcttg cttttttacc atctctgtat gcttggttca atataaaggc
      301 atcctgggac ccttatggaa gcactgccaa tatgcttgta gcagtaacaa atgaagataa
      361 aggaaatgaa atagcagggc aaaagattaa tgtaggagac caattaattg aacaactcaa
      421 agataacaag agtttaggtt ggcagtttgt agatgaaaaa actgctcttg aaggtgttaa
      481 aaaaggtact tattatgcat cactaaagat tccaaaaaat tttacaaaag atttgacatc
      541 tttaattaca gatgatgtta aaaaaggcga aattatctat actgttaatg aaaaaataaa
      601 tgcagttgca ccaaagatta cagataaggg tgcttctaca atacaactac aggttaatca
      661 gactatagta aaaacagtta gtgaaatcgt tcttggtata tcaaacggag ttggtgcagg
      721 aattgaacac agtcttccaa aattgacaaa agttgaaaat tcccttattg accttcaaaa
      781 gagatttgac catataaata agactgttga tttagcttct gatgctactt caaaattatc
      841 tgatacagtt aaagatatta aatctgattt acctacaatt aaaaagactc tgaatgatac
      901 aaaactgtta tcaagtgact taaaaaaatt cttagaggat acaaatgata atttagatga
      961 actttctcct cttattaagt ctgacttaaa tttaatggtt gacctttcat cttctgcttc
     1021 ttctcttaca ttaaatttaa ttgatgcagt aaatagtggc agtgaagatg tacctaagtt
     1081 gatagataat ttatcagaaa aactttctaa tttgcaatct ttaaacgata ctttggtgga
     1141 ttttttaact aaattaaacc aacttacatc aaataataga ttagatgatg taatagataa
     1201 tcttgaagac tctagcaata aaatagattc atctatatca acactaaatg atattaaaaa
     1261 caaagttata tctggacaac aaccatctgt aagtgttttg aataatgttt taagtctatc
     1321 aaatggtatt ggaagaatta atttaaatat tttaaataat tttgactcaa aaatatcaag
     1381 acctataaat agtatatttg caaacagtat aaaagtagca aatgatataa ttactgtttt
     1441 agataaggca gaagcaaaac ttccaaaggt tgaagaaata cttactactt ctctaaaact
     1501 ttcaggaaat gcacaggaaa gtatatcatt gataagagaa agacttcctt tagcaaaagg
     1561 gatgttggat gatttgattg atactttaag caagattagt aatggtgaag atatgaaaaa
     1621 attagtcagc ttgcttgaaa gtgatatatt gacaaagtct aatttcttaa aagaacctgt
     1681 tgaggtgaaa acagagaaat tatatccaat tgaaaattat ggttctgcaa tgacaccatt
     1741 ttatagtgtt ctttctacat gggttggtat attacttatt tcagcactat tgtctacatc
     1801 tgtaaatgga aactataaac cttatgaaat ttactttgga agaggcctta cttttttaag
     1861 tatagctctt gttcaaggtt ttataatcgg aactggagat atattattat taggagtaac
     1921 agcgaaacat ccaattttat tgatagttct gcttatgttt actagtatgg tatttaactt
     1981 tatagtgtat tctttagttt ctgtatttgg aaatgtagga aaagcaattg caatagtact
     2041 actagtcgtt cagattggtt cttctggtgg tacattccct atacaagtta cacctacatt
     2101 cttccaaaga ataaacccta taatgccatt tacctatgca ataggtgcta caagggaagc
     2161 agttggtggt atatatgctc caaatctaac taaagatata ttagttctat tactatttat
     2221 gattctgtct atttgtttaa atgtacttct aaaaggacct ataaatagag ttataaaacc
     2281 atttaatgat aaatttggcg atagtaattt aactggtcat taaaaaatta attctgtatt
     2341 taaatatatt ttttaataat attctaagtt catataaaaa ggtagtagct aaaaagctac
     2401 taccttttta tatcttaaaa actaatactt actacaaaaa ctgtcataga aaaaagcgaa
     2461 aaaattcgtt ctttcctaaa tttatcttat aaatatttag atatattcct acttaataag
     2521 cagagctata catagtattt aatgtataac ttacattatt taactcatat aatcaattat
     2581 tcctgagttt aaattatata aatgctcaaa accttcttca gctaacaaat tacatgcagt
     2641 aacacttcta tgaccacttc tgcaataaac tactactttc ttatctttat aatcgtaaat
     2701 ttcatctata ttattgatta attcttgtaa ggatatattt atagcatttt ctattttata
     2761 ttctctaaat tcatctttag ttcttacatc caataataaa atatcctttt catttttcat
     2821 catttcttta agttgattct tatttatatt cttgtacata tgtctaaaat tcctccattt
     2881 ttatttatat attacctata ccctttattc tttatacaac aacaaatatt atttttatat
     2941 ttttctctgt tatataaaaa gctatactta taaaagcttt ttttattgat aataatctgt
     3001 tgattaaaat aattaagaat gttatgctaa ttataaatta ttaaaaaggg aaagaggtaa
     3061 tgtaatatgt ttaagcaaaa gctctctaaa ttattatcta gtacattagt attgagtatg
     3121 ctatttactg ctgctccaaa tattactttt gctgataata caaaagataa ttccgaaaaa
     3181 tatcaaagtt ctgatataga attacatgat tatagtaaaa attcggaatc ttacacaaaa
     3241 actaaagctc ttgccaaaga gaaaatccaa actttgctat caaagtatgg tgctgtaagt
     3301 gctcaatatg ccttgattga taatggtaaa attgagattt ctggtaatgg tggagtttac
     3361 agcaaacaag ataacaaaaa cttaaataaa gataacatgt atagtatcgc atctatcagt
     3421 aaaatgttta ctacaacagc tgttatgaag ctggttgatg agggaaaaat aaatttagat
     3481 actcctgttg taaattatat acctgaattt aaaatggctg atgatagata caaagaaatc
     3541 actccaagaa tgttattaaa tcattcttca ggattgatgg gaagtagttt taaaaatact
     3601 attttattag gagacaatga ttcttatggt catgataatt tcttaaaaga attgcaaaaa
     3661 caaagattaa aagctaaacc aggagctttt tctgtttatt gtaatgatgg atttacttta
     3721 gctgaaattt tagtagagag agtatctggt atgtcattta ctaattttct tgataaatac
     3781 ataaataatc cacttaactt acaaaacaca aaaactccag aaaatagttt tgatagttca
     3841 aaactagcaa aagcatatgt accttattgg gaagatgcag ttccacaaga taaccttaat
     3901 gctataggtg caggtggttt atattcaagt gcagaaaatc tatgtacatt tgcacaaaca
     3961 tttatgaaaa attcaaatgg tattctttct cctgcttcag taaaagctat ggaaaacaaa
     4021 gaatatttaa atggtctatg gcctgaagga gaagactcta tacttggata tggtttaggg
     4081 tgggattgtg tcaacagtgc gacaagttgc taattgaaaa gattagccaa taatgattta
     4141 ttaattattg aactgatacc ttgaactgtc gaatgataga gtaacgtctt gaaaggcagt
     4201 cgctgatact tcgaatagcg ttcaattgat tgggaattga aggttataag ctcgataaag
     4261 acagctgaga gtgtacccta aggttctata tgattcgcta tgtcgtgtga ctaggttcta
     4321 caggtctgaa ccttacttat agaatctata gagaaacagg aaatgtaact agaggtagtt
     4381 aagtacatga taggctgggc gtctataaaa tagtcatggt tagaatagta ggtaaaaact
     4441 actgacgaat ctccgaatgt acggctctaa acacaaacat tatagaaatg taatgggact
     4501 taaacagtag accttgaagt agagtaaaat ttgttgatat gaaatacttt ataatgttaa
     4561 aggcattatc aagaggtcag ggtcagagga gaaacctaaa actatatggt aaaagataaa
     4621 ggtattggaa cttgggaagg gagcaacgct gagataagcc taatacaaag tcaatgaagc
     4681 gttcagaagg aaatttagaa tattttgatg taatatattg acaaattttg gtaaatatgt
     4741 taatctatcc ttaagtataa aatgctttaa tgggtttttt atgcaaaaat ataataagga
     4801 aggttaaagc catgaaacga ataaagaaag tttttagaga tttagtggaa aattacaaag
     4861 aggaaaagtt tgagaaacaa gaggctattg agatgcagga agctatagaa gcaataaggc
     4921 aagaaattgg taggcatgac ccaacaataa atactccaaa ggattcagca ggaaatgttg
     4981 gagatgaacc acccttcatc tccaacccgt tggtcgctaa ccaacccata aagaatccca
     5041 aatataaagg taattaacct ttatattaat agttaaatct ttgtaatagt acatagggaa
     5101 aaaaatagag aaaatattaa atattttctc taaaccctaa ctaaaaaaaa aaaaggaaac
     5161 aaacacataa ttctcaaaaa cttactttta atatatatta caaaacatta tataaacaat
     5221 acacactata taatgctaaa gcatattaag gaggatgagt tctctatcct ccttatacaa
     5281 catcaaaata tgtacaaatc taatataact tctgttaatg tctcctgaaa gcgaagccat
     5341 agtaccgttg aagccatgat aataagtggt ggagggatgg gctttagcca acaatctaaa
     5401 aattatctat tatttcaaat tacattaggg ttcgagtacg attaagaaag attatattcc
     5461 ttataaatat atacaaagga gataatcgac ctatacgatg tataatacag atacgaagtc
     5521 aataattaaa caggatttac aaccattaaa gaaagacaaa ctaagatata atgaatatta
     5581 taacttgcaa aatacattcg acaatttgta taaacaaagc cttgaaggaa gtaagttcac
     5641 caaattattt aatttaatat ctagtgaaga aaatatatta ctagcttata gaaacgttaa
     5701 aactaataca ggaagtaaaa cagcaggtac aaatagacac aatataaatt actggaaaac
     5761 gaaagatact aaaatatttg tccagtatat acaaaacagg ttaatgaatt acaaacctca
     5821 aaaagttaga cgagttgaaa ttccaaaacc taatgggaaa ataagacctc ttggaatacc
     5881 ctgtatagaa gatagaataa ttcagcaatg tataaagcaa gttctagaac caatatgtga
     5941 agcaaagttt catccacata gttatggttt tagacctaat cgtggtacgg aacacgcttt
     6001 agcatatgta gttaagaaaa ttaactttga acatatgtat actgttatag acattgacat
     6061 taaaagtttc tttgataatg ttaagcatag caaattatta aaacaacttt ggtcaatcgg
     6121 tatacacgat aagaaactcc taagtattat cagttcaatg ttaaaagctg aaatcgacaa
     6181 agttgggatt cctaacaaag gagtcccaca gggtggaata ctttctcccc tactatcaaa
     6241 tatagtttta aacgaattag actggtggat aagcaaccaa tggcaaacac ataaaactca
     6301 ttatcaatac tctactacaa gccatttata cagagcattg agaaaaacta acttaaaaga
     6361 aatgtatata gttagatatg cagatgattt taaaattatg tgtaaaaatg ttagcgaagc
     6421 taatcgaatt tttatagcta cacaaaaatg gttaaaagaa agattaggat tagagataag
     6481 ccaagaaaaa tccaaaatca cagatgttag atattctaac tcagagtttt taggattcaa
     6541 aatcaaagct tataaaaagc gaaataaata tattgtttat acaaaaatga ctgacaaagc
     6601 taaagagcaa gtcagtcaca atataaaaga acaattaata gctatacagc attttccaac
     6661 aactaagaat atcaatatgt tgaatagaat tattgctggt tctcataact attataaaat
     6721 agtagcgaat gtatatcaag actttaagaa cattaaatat aatctttctt tcagtttata
     6781 taatcgcttg aaaaatctaa gaacttctac aggaaattta acgcatgaat atgaaagtaa
     6841 atatagtaaa tataacttaa agaagcaata tatactaaat gaaatcatgt atcctctaac
     6901 tggaatcaaa acgaccccac cgtttctatt taaccaaacg gtatcaaatt atacgaaaga
     6961 aggtcggaaa ataatacata caaggttagg ttgtatagat aataatatac taaactatat
     7021 agcacataac cctatactta ataaaagtgt agaatataat gacaataggt tatcaaaata
     7081 ttcagcacag aaaggaacat gtattataag tggttatcca ttagtagaaa atatggaagc
     7141 acaccacatc acaccagtca acaaaggagg tgatgataaa tttaggaatt tgattttagt
     7201 tataccagaa atacataaac ttattcatgc aactaatatt gaacacatac aatattatat
     7261 aaaggaacta aacttagaca atcaagaaat taagaaattg aataaatatc gtaagaaaat
     7321 cggaaatgaa ataatatgat tgatagattc ttgggggaac gccgtatgag gtgaaagtct
     7381 cacgtacggt gtggagcagg ggaaaagacg gagatagcat caaactctta cctattgcta
     7441 tcttatccat ttaatcagta taatttaaaa gctcttacaa aaggtggaga ctctctatta
     7501 ttccacagta atcttatagt tcttccagat gaaaatatgg cagttgcagt tctttcttct
     7561 ggtggaagta gtcaacttaa cgaaataatt ggacaagaaa tcttactatc tgcattaaaa
     7621 gaaaaaggta aaattaaaga aataaaacct gataaaacat ttagtaagcc acaacaagtt
     7681 aaaatgccaa gttctttaaa agaaaatagt ggtctttatg cttcttcaaa tatgataaaa
     7741 gtagatgtta atgataatgg tactttaaca gtatcttctc cttatataga aaatggacct
     7801 gaagataaat acgtatacat aggtcaagat agatttgtat ctgaaaaagg taatagttgt
     7861 ttaaaatttg taaaagagaa aaataatatc acatatttaa atatgagctc ttatgatgat
     7921 gtccctggat taggtcagac agcaagttta tactatgtag ctcaaaaggt agatgacaac
     7981 aatatatcaa atagtgtaaa agaagtgtgg aaaaaaagaa atggcaaagg ttactatcta
     8041 gtggatgaaa aatatacttc tcaaagctat atgtttggtt ctgtgaaggc aactcttggt
     8101 ctttctgatg aaactccagg ttatatagtt aacactaaga taatagatga gaataactca
     8161 aatgctttta tagaaatacc tggtgtaata ggtcgtgatt tatccgatat taaacttcat
     8221 aaagaaaatg gtacagagta tttatctttt ggcacactaa catatgttag tgaagattct
     8281 ataactaatt tgccagcaga aaaatctttt acttgtgaac tagcatccaa tggttatgca
     8341 aaatggtata agattggtga cgatatagca aataaaaaaa tagaagttaa tcttcctcaa
     8401 aattcatctt ttgctgttta tgatgataaa ggtgttcctg taaactactc tttagttaca
     8461 aagaacaata gagttagatt gccaaaagga ggcgtaattg ttttcttagg aagtccaaat
     8521 gctagatttg aagttacata tcaagatgag gtaaatgcaa gtgcacttac aggaactgat
     8581 agatatgaaa cttctattaa gataagtcaa gcaggttggg aaaatgctga aaatgcagtt
     8641 ttaataaacg actcagcaat tgcagatgct ttggctgcaa caccatttgc atacaagaaa
     8701 aacgcaccta tattattgac tggaagctct caaataaatg aaaaaacttt ggctgaatta
     8761 aagagattaa aagttaaaaa tgtatatgta gttggtggag aggcttctat aaatgaaaaa
     8821 tctttagata ctataaaatc aaacaatatc tctgtatcta gaatttcagg aagtgataga
     8881 tatcaaactt ctatgaacat tgccaaagaa ttgaataata ttagcaatat atcaaagatt
     8941 tcagtagtta atggtgaaaa aggactagcc gatgcagtaa gcattggagc tgtatcagct
     9001 caaaatgata tgcctataat acttactaat gaaaatagta atataactga aataaataac
     9061 ttgtttaaaa acaaaaaaat tgataaatct tatgtaatcg gtggagaata tacggtttct
     9121 aaaaatatag aaaataaact tcaaaatcca catagaatat caggtagcac tagaaatgaa
     9181 actaatgcaa aagtaataaa agaattttac aaggattcta agatagacaa cttatatgtt
     9241 gctaaaaatg gaatgaataa acaagatgat ttaatagatg gattatctgt tggagttttg
     9301 gctggaaaaa ctaaatctcc tgtaatgcta gtaggaaact cactagatta taatcaaaaa
     9361 gaactattta aaactatgag atttaaatct gtaacacaaa ttggtggaaa tggaaatgaa
     9421 aactcattta aacaaataaa agacatagct tagcataact aaaaaaggtt atattcaagt
     9481 aacattaatt gaatataacc cttttttatt tcagttataa attttatatg tgtaaagata
     9541 aaatttatat attacatgat aagtttatta aaatataaat aattctaaaa actttgcctt
     9601 ttttaagtta gtttctattt gtcattcata cttaacaatt tatctaaata tactgacttt
     9661 aattgtttac tagctaaaat ctttttagct gcttcaagct ttagtatagc tgatagtata
     9721 gctcccatag ctctatgact agctagagca tcattatcaa aaattaaatt ttgtaactgc
     9781 cctttttcaa ttgccatgag aactgctcta tgtactgaat ttgctggagt tattattttt
     9841 tcatctctct ttaataacat aatgctattt ttatggcaat ttctaacaca aactccacaa
     9901 cctaggcatc tatcctcatc aatttttata tattctttac catcttcctt aaccttgctt
     9961 atcgcatcta ttggacatgc atttatacat ttaccacact ttacacaatt ttcatggttt
    10021 atatttggta taaaactggt tgtctgtact ggatgaagat ttccaaatct tttagctgct
    10081 accattgctt cacagcaaca tccacagcaa ttacatataa acgtaacccc ttttctaaca
    10141 ttttctccac attgaactaa gttatgctca tatgcttggt gaagtagttc tttacattct
    10201 attttatcca cgcgtcttgc aaatttatta tttattaaag aatttgctac attgtcaaac
    10261 gtcatacata tatccatagg agcatcacaa gcttttccaa catgttgcat tctatgtcta
    10321 cagtagcaca tacttatacc tatatgagtt gattcatcaa ttatatgagt agccctttca
    10381 taatctaaaa tactaacttc attctcatta gttaaaactt cctcttgaac gtatactctt
    10441 ccaagcttcg tttctgttga ataaaataaa tctttgataa aatcttcttc tacattcata
    10501 tattgatagt aaagttctgc taaaagtttt tggtctatat cgtgtctcgt tctcatcata
    10561 gcaaactcaa aaaaaccagc cattggagga ggcattatat atttcttccc tttattatct
    10621 tctatatcta gtatcagagc tttacttgca agtttatcca atactctata tgcttcactt
    10681 tcacttacac tccaaatttt agctgctgtt tttactctga aaggctttat tggaagctga
    10741 gcaactaact tcgcctcttg ttctgtatat agtacattta gtatcttgta taatgtatct
    10801 gatggtggag caccttgtgg aaacttgttt attctttcct ctaaactttt atacatattc
    10861 ttagcagtta tatgagaaat agtaatcacc ttctttttct tttgttttcc aataaaatta
    10921 aatattcttt cttcttatat agactcttac tttatataat accaattctt tctaaatagt
    10981 taaatacctc tagcaattta ttattagatt tcttatataa ataaaaaaat cgtttaagta
    11041 gtataataat agtttatacc aattaaacga ttttaataat agtttatata attaaaaatt
    11101 atttttatat ttatatactt tctcttctta tattatctat tatattttct ttatttaatt
    11161 tactcattgg tatataccct gctataaaag ttattataaa aatacttact atacagataa
    11221 ctatggctgt ccacggtata atcggagaaa attcaattac acctgaaata agtttattca
    11281 taattagaat tataccaata ctaattggta ttccaaacaa caatgacagt ataccatata
    11341 taaaactttc taaatatatc atttttctaa atcctttagg agtaacccct atagattgta
    11401 ttactgcaag ttctcttttt cttagattta tacttgtgct tactgtattt agaatatttg
    11461 taacacttac taaagatatc actgctataa aaccatatac aaatatttta ataactttta
    11521 aatcttgttt ttcactttca ttgcttgcac tttcatcgta gaagctaaaa ttatttttat
    11581 ttgccaaagc ttctatttcc ttcttagtct taatgcttcc tcctgaatca ataaatataa
    11641 ggcttttact taattttaaa tttagatttt tagcaacttc atcataagtt ataaattgta
    11701 atcccatatt ttgataacca agacctaaac gttcttcatc agttatagca agaatcttga
    11761 cctttatttt tttacttaaa gcattttcat caagatattc acaatttact gtatctccta
    11821 ctttatagtt ggtaagttct actacacctt tcttcccatt ttcttcaaat gcgctttttc
    11881 taactaatat aacaccattt tcttcaattg ctctctcctt attaaaagaa ccctgtatta
    11941 agttatttgt tttgtttttt actgcaaaat ctcctggaaa aattaattca ttattactga
    12001 aatcatattc ataactatct ccaatcttat atttttgata atatttcctg attaattctt
    12061 ccttggattt atttatttta ttttcactta ctcttataga catatattgt cctgttgcca
    12121 attcaaagtt ttttattcca gaaatagctt ctaatttact tatagtatca tctatagact
    12181 tagctgtacc ctttgcagtt aaatacaaat catagtttct ttgagaattt cttatttctt
    12241 caccttttaa taaaagtaat gtaaatccgc taaaagatat aaatattatt atacttacca
    12301 ttaaagaaaa aagagttact gtgaattttt ttctatttct ccttatattt ttatatgcaa
    12361 gtactccttc agttttaaaa atcattttaa taagccttga acttttaact tttcccactt
    12421 tatactctcc attattttta attacattaa gtggagaaat attagaagcc gatattgcag
    12481 gtaatagaat tgatataaat attgtaaaca aaactattat tacactaaca ataattatta
    12541 ttggattata cactatcttc aaactcattt gagtaacaac cgattctgta aaatgcttat
    12601 ttataatttt aaataataag tctatagcta ctgttccaga tattagtcct attggtatac
    12661 caattaaact aattataata ccttctataa aaactaatct tttaatttga ctagatgtag
    12721 cacctataga atttaatatc ccaaactgtt tttttcgttc agttatagaa atactaaaag
    12781 aattatacac tgtagcaatt gtacatatga ttactaaaat agttactgct ataattatag
    12841 actttataga gttatttata tttgcatact tactagcacc ttgtagcctc aataaatgtt
    12901 cattgtattt tatcatatca gaaactgctt ctttatcttt atttgaatat atatttttac
    12961 ttattttatt acttatttca tacacatctt taggatttct tagtgctact gatacattaa
    13021 ttgtatcatt gtaattatcc ataggattaa aataagttat agcagttgca attccattgt
    13081 aacgttcaaa tccaggtttt ttaattatac caacaacttc aaaagttcta tccttttcat
    13141 ttgctatata atccccctca tgaaatacca tactatcaat cttcttctta cttgaatcaa
    13201 acatatctcc tacttttaaa gttatcttat cccctatttt taagttatca tctataagat
    13261 taataatatt gtcacttaga acaatttctc cttccttagc tggaaatcta ccttctttta
    13321 tagaaatttt atatccgttt attgcatttt tatcatattg ctttatactt actatgccat
    13381 tttcactatt ttctaattta gaatatccaa gctgtttggt aaatgcatgt ttttctactt
    13441 cagctgattt cgttacatat tccacatttt ttctatttac atcataaaat gtaacatgga
    13501 aacttccatc attctttata gtttctctaa tttgataatc cataaaactt tcaaatatat
    13561 ttcctatacc acaaattaaa gctgtcgaaa gtattatacc gattatggtt acaatagttc
    13621 ttcttttatt ttgttttaag tatctaatag ttagtgatgt atataaattc atctatatca
    13681 cctcatcact ttttatgatt ccatcttcta tacttataat tctatccgct tgaagtgcca
    13741 tatttttgtc atgagttatc attattagtg tctggttgta tttttttact gataatttta
    13801 acaattctag tatttcttta gaatttttac tatctaaatt acctgttggc tcatctgcta
    13861 atattataga aggtctatta atcagtgcac gtcctataga agttctctgt tgttgacctc
    13921 ctgataattg gtttggaagg tgcttttctc tatcaattag acctaatgtt ttcattaaat
    13981 catcaaaata ttccccatca atatctctat tgtcaagctc tgctggaagc aatatgtttt
    14041 ccttcacatt taagactgga attaagttgt aaaattgata tataagacct atatttcttc
    14101 ttctaaatat cgccaaatcc ttagttttta aattatatat atcaacatca tttatataaa
    14161 ccttaccact agttggcctg tcaactcctc ctaataaatg taataaggta cttttaccac
    14221 ttccacttgg acctgttatt gctataaatg ttcctttttc aactgataaa cttacatttt
    14281 ttatagcatc aacttttgtt tcatttttgc cataactttt agttagattt tctactctta
    14341 aaatttccat aactacctcc aatatttata ttctatactt catatattag gctttggcta
    14401 ttactataaa gtgaattaaa tattacagat tagtcactat catacatttt atgaaatatt
    14461 atatgaaatt ctgtaccctt acctttttcg ctgttcacat atatatcgcc attttggctt
    14521 tctattatag attttgacat tgcaagacct attcctacac tatcttcttt tgaactactt
    14581 ctgcctctat aaaatcgttt aaatacatgt ggtatatctt ttttatctat accttcaccc
    14641 tcatctttta ttattagttc agaaaataat ggattttctt tataggttat aattatattt
    14701 ccaaattcag gagtatgttc tacacaattt ttgattatat tcactagtgc ttccacagac
    14761 caatcaatat caccaatata agatacatta tcatttccct ctatagtcaa attttgattt
    14821 ttaatctcca taggtatttt catagattgc attgctctat gtatgagttc actaaatttc
    14881 accttatcct ttttaaaatt tatcactttt gcttctacct ttgataattt aagcatgctt
    14941 ttaatcagcc aatccattct atttaattga ttttttatct tatttaaaaa atcgattttt
    15001 acttcatatg gtacatcatt atataataaa tcattcaaca ttattaaaga agtcatagga
    15061 gtttttaatt gatgtgatat atcggaaatt gtattgttta aaaatatctt ttctcttttt
    15121 aaaagttcaa ccttttcatt aagaattgtt gtcatcttta agagctcagt ttttagaaga
    15181 cctatctgcc cttcttgatt cttgtttttc atatcaaatg gcttaccctc tgaactatag
    15241 taaacatact tagtcatatc tgataaatca ttgtaaattg acttaaaata tctaacaacc
    15301 aacacaagta tcaaaataaa tattatacat actaatatta tatttatttt gatagtctct
    15361 aagactaatt tagaaataat aggctcagag tttatcttaa tagatttatc ataattatac
    15421 ttacttaata ttttctgacc ataatctatg tttcccgtag acttgccttg agttattata
    15481 tctactatat cactttctaa gtttggatat ttactcgata aagtccctat tatagcttga
    15541 ttatttctta caaccatatc tttagttaaa gacacgttaa ttacactaaa tccaatagat
    15601 attatgataa caataaacat taaagtaaca tacttactta aaaacttctt cacttctgga
    15661 ttatatagat acactatttt cacttcctat ccatttatat ccaatccccc ttacagtttc
    15721 tatgtagatt ggttcacata aatcatcacc tattttttct ctaagccttt ttatataaac
    15781 tgttagagta ttgtcattta caaaatcata agttacatcc caaagttttt ccaatatttg
    15841 agttctactt aaaacagtat ttttattctg tataagaatt aaaagaagtt tatattcaac
    15901 tgatgtcaga aatatttctt tatcgttttt atatactcta gcttctaatg tatatatact
    15961 taaatctcca aatttaagta ttttcttact ttcttctacg gtattaacct ttcttctcaa
    16021 aacggctttt attcttgata ttaattctct aattctaaaa ggttttgtta tataatcatc
    16081 tccacctata tcgagaccca ttacaatatt tacttcttca tcacaagcag tcaagaatat
    16141 tataggaacc tgagagaatc ctcttatata ttgacaaagc tcataaccag ttccatctgg
    16201 caatgtaaca tccaacagta tcatgctata ttcattagaa gaaatcattt cttttccact
    16261 agataaatcc tttgatatat ctatactaaa tccttcttgt tctaaagcat actttattcc
    16321 aaaagatata gtactatcat cttcaactat caacaacttt aacataacta tttcctttct
    16381 tttcatttta attttaaact atctgcttaa tttcatacta tctatctata caagttttat
    16441 tatacacaat taccctggat actaaaatat ttctaaaatt acaaaatagt cacaaagaac
    16501 tattaaaatt tataaaaata aaatgtaagt tgttacttaa ctatctaaag aatgatactg
    16561 attctttcaa gttaagttac aacttacata ttaaaactag atattaaata atttgacatt
    16621 actatttgtt cagttataca aaatattact tattcttctt catatttttt gtattcatct
    16681 tctttgtaaa tattatcttt tttagcatct tcatgtaaat caatctcttc ctttacatct
    16741 ttaacactat ttacatattg catcatttct tcttgagaaa catgtggaca attttcacaa
    16801 tttattcttc ttgaattaaa ttcttttact ttttttttta attcttcata ttcataatct
    16861 tgttttgtgt ctctacaatc acagtcataa tcaaattcgt catatttata atcatcataa
    16921 tcgctatttc cataataatt tgtattgacc atttgagaat tagatttatg gctttttacc
    16981 attttatata cagcatatgc tcctacacct gtaattacag ctcctgtaat catcattggt
    17041 ttactcattc catcatcttt tttatgcatt tgtcttttca ttaaatatgt taacatattt
    17101 tttctccttt ctaattttta tttctatcta taaatagtat ctgtattatt ttaataaaaa
    17161 ttatgttatt ttttaaatta caatttttac aaaatataaa cttatatttt gtgtttgtta
    17221 ttatttttta tgttatacta tgtatataac aatcgttttc atttataaag cataatggag
    17281 gttttttatg gttttaaatt tagattttaa tagtgataaa tctatttatg tacaaattaa
    17341 agaagaaata actaaggcta tagcttcagg agaattgaaa ataaatgaat cacttccatc
    17401 tgtaagaagt atggctgaaa atattggtgt aaatttacat acagtgaaca aatcctacaa
    17461 tgaattaaaa gaagagggat tcttaaatat tgatagaaga aaaggtgcta ttgtagctca
    17521 actaccaatg aaaatagata atacaactcg tcaaaaaaat aaacttgcct tggaattatt
    17581 ggtggcaaaa tcttatttat caggtattag taaagaagaa tttttgtcac taagttcaag
    17641 tttatttgac aagtatgagg tgaaatatta tgagtaagat tgaatgtcta tttacaatat
    17701 ttccagttct aattttgatg tatgctacat tttattttat gccctatttt gtaggtaaaa
    17761 aacacatcta tggagtttct atagaccaag agcataaaaa ttataattac tttataaagt
    17821 tagataaaga atttaaaaaa ctattatctt taggttttat tatagacttt atattagttt
    17881 ttattctggt ctttacattt aataaattgg aactttccta ttttattggt attataggtt
    17941 ttttattata tgaaagtata ctatatattc atacacataa aaaagctaag aagcttaaat
    18001 cagaaattta ttctaaatta ggtcatatag atatagattc aaaattaata gtagatatgg
    18061 attttataaa taagaaaaat aaaataataa aaaaatttaa aataatctat ttaataccga
    18121 tactatttac tttgggaatg agtattttta tagcatttaa ttataatcaa ttgccagact
    18181 caattgccac tcattggaac ataaatgggt ctcctgatgt ttttatagat aaatcatttc
    18241 ttaatgtatt taaattaatc ggtactgact tttgtttaat ggttttatta tatataactt
    18301 ccattggatc cataaagtca aggattaaga tagatacaaa tagaattgaa gaaagtagag
    18361 ttgaaaacac taaatattta aataaaattg gttatttgtt tttaatattg atgattataa
    18421 tgacaacaca gttttttact actcttttat caataaaaac aaaactatca actgctatga
    18481 atattacaac gcttttagtt ataatttatt taatagcaac atatattaat tccccaaatt
    18541 taaaattcaa ctcatcttat tctccagaag aagatgagaa gtattggata gcaggcatca
    18601 tgtataataa tccaaatgac ccatcattaa tggttaataa aaggtttggt attggatgga
    18661 ctataaattt tggcaatcct cttggaaaaa tactatatat tgctatagct ttacttttaa
    18721 ttttttcatt gtttagccta ataaaatctc tcttacttta aaataccata aaaggtatct
    18781 tacttgtatt attgggtatt atattttgta ggataagaaa tatttcatat tatcgttgaa
    18841 gtatagtatt tatatattac taagaatgga gattttcata atgttgtcaa aagctgaaat
    18901 caaatatttt aatgaatgtg cttctgagat actttcttct gaaaaagttc aacttatgag
    18961 aacatttcct catcatggca atgtttcatg tcttgaacat agtttgtcag tagcatatta
    19021 tagttatcta ttatgtaaaa aattgcactt aagtgttgat attcaaagtg ttattagagg
    19081 agctttacta cacgactttt ttctttatga ttggcattac aaaggtgata gaaaaggatt
    19141 acatggattt actcacccta gagaagcatt aaaaaatgca actttatttt ttcaaataaa
    19201 tgaaaaagaa acggacataa ttttaaaaca catgtggcca cttactgtta agccacctag
    19261 atacaaagaa gcatttattg tatgtttgtt ggataaattt tgctgtttag ttgagacttt
    19321 aaagatacat tctttattat ctccttacca tgtataggac aattcccaca acacttttat
    19381 aacttaattt tgaagaggta ttaagtatta tattcaactt gatacctctt tactatttat
    19441 tattttatat aataatattt tctttataat atagttataa ataacaactt ctaccctcga
    19501 taacttcccc ttcttataga catgcttact atcttaaata acaagaaacc agctattaaa
    19561 tttattagta ttgtttatac tactctaaca ataatatcat aaataaacct ccaaatagct
    19621 tctctttcac taccttttat cttaaactat atttttttct taaaatttgt aaaaaacata
    19681 gcataatctt tataaatgtt ctaaaattta atttaagcat caaaattaga aaggatgata
    19741 ttatgtttaa agaaatgaga ttgaaaaaaa gagaaatgac aaaagaagat actgttgaag
    19801 tattaaaaaa tggtgaattt ggtacttttt ccactatatc tgaaaatggt tatccttatg
    19861 gagttgctgt aaattatgta tactttaatg attctattta ctttcattgt gcaagaaatg
    19921 ggcataagtt agataatata tcgaaaaaca acaaagtttc ttttttagta gttgctaatg
    19981 aaagtgttat tccagataaa tttagtacta cttattctag tgctatagtt tttggaaaag
    20041 cttgcactgt agaaaatgaa gaaaagaaaa atgctcttgt agaaataata aaaaaatatt
    20101 ctaaaggatt ctttgaagaa ggcatgaagt acatagagaa ggatatgaac ttaacaactg
    20161 ttgttaaaat agaaattgac catatttctg gtaaagcttc acgtttataa ttaagtaaaa
    20221 atacctaaaa gtatatacag tcgcttttag gtatttttat atgatattct tatatgttaa
    20281 gtctttttag ataatctctt atacccatct tggcatcatc atttggtctt atatcagaat
    20341 tatctcctgc tatatgaagt attccatacg ctggataacc tcctcttact gaacatggaa
    20401 ctgacaaaag agtcattccc atatgttggg caaatatatt catagcttgc aaagctaatt
    20461 ctcctcctcc atgttgtgaa cctgctgtag caaaacaacc aaatatttta ccactcaaag
    20521 gagcacctac ccaaatatca gaaaaactat ccataaacat cttcattggt ccagatacat
    20581 ttccataata tgttggtgaa cccataaata tagcatcata atctaatatt tcttttccag
    20641 atttaattac atttatactt tcaaattcgt ctttgtattc tttggaattt atcaaataat
    20701 attgaggaag tgtttttgca acttcgtcac ttactctaaa tatatctacc tcgatattca
    20761 tttcttcaag cgcttcttta tattctctag ctaataaata tgtactccca caaacactat
    20821 gaaaaattac tgctactttc atcattatcc ccctaattaa actcaaattt attttttata
    20881 tagtattttt attatcaaac actgctaaat agtactaaat ggattcaaat atacattact
    20941 ctttattact tcaatttcac taaattcatc taaaaatctc ttcataacat ttttgaatat
    21001 atcttctgta tcaaaatgac cacaatccac tatacacatt ccaatatcaa gtgcatcttg
    21061 tgcctcatga tactttacat cacccgtaat tagtacttct gcaccttgtc tcttagcttt
    21121 ttttacaaat tctgaaccag ctcctgttac aacggctact ttcgttatgt ctgtactaag
    21181 gtttccaact actcttatgt gtttcatgtt taatttttct tttattttcc ttgaaagact
    21241 ttctaatgtc atacttttat caagtttact tattctacct agtccaaata tctcaccttt
    21301 attttctaac ttgtacaaat catatgcaac ttcttcataa ggatgtgcat ctaacataga
    21361 acttattacc ccacctaaaa gcttctgtgg tactacagtt tcaattttaa cttcatttac
    21421 agtttctata tcatttacac ttccaagaaa cggattagag ccttctaatg gcttaaattg
    21481 tccttcacct tctattgaaa acgtacaatc actataattc cctatatgac ctgctcctga
    21541 attacttaaa acctttctaa gttcatctga atagttatgt ggaacgtata ctgctaattt
    21601 atacaaattt tcgcttttag ttatatctaa aacctttgaa ttaccaaatt ccataatttc
    21661 cataaaatag tcatttagac catcaaatgc tatatcaaaa ttcgtatgca tagaataaag
    21721 tgatatatca tttttaataa gtttttggat aagtctgcct tttaaatccc cactattaat
    21781 tttattaatt ttcccaaata taaaaggatg atgtgtcaca attaaatcta tatttttgga
    21841 tatagcttca tcaataacat cttcattagc ttctaaagat accaatacct tttttacatc
    21901 catatcaaaa tctccaacta taagacctac attatcccaa tcctctgcta agtttagagg
    21961 atactttttt tcaatttttc tagttagact ctttaatagc atatatacct cctatttatt
    22021 ttattaaatt ttcaagtttt tttattgcaa cttcgctttc ctttctcttc ttatctatag
    22081 cttccccatt tttcccttct aatttattaa ctatagaact atatgtcttt atcttttttt
    22141 ctataaaatc attaaaaaga gaatctttgt tttctaaaag ctttattcca acctcataat
    22201 atatctcatc ttctattata gtatttttac ctgtatattt ggcaacaatt atctcatata
    22261 ttctaaaatc ttctctcact aaaacttctt ctaaaatttc atatccgttg tttaaaaggt
    22321 aatatctaag ttcttcttgt gcttgcattg gctgtaatat taatttttct acattatgtg
    22381 ctacttcttt tttagcttcg agaagctcac ttatcaagat tccacccata ccagcaatta
    22441 tcacttcttc tacttcccct atttcaagta tctctatacc agaacccaat cgtaaatcaa
    22501 ctttatctaa aagattgttc tgtatgactt ctttatgtgc attgtcaaga ggtcctttat
    22561 taacatccgc tagcactgca aatggtactt ttccctcttt taacaaatat actggtatgt
    22621 atccgtggtc cgtccctata tcagctattt ttttaccatc tgacactaat gatgctattt
    22681 tcaataatct atctgttaat ttcaaatata attctccttt caattccctt tatttatagg
    22741 atttaatcca agttatagat attagactga aataactaca aaaataaaat taaatcttta
    22801 ctacaaaata gccatgagat atcacttatc tcatggcatt tatcacaaaa acatcaatga
    22861 tgtatctttt tagtctaaat aatctctaag tttttttgat ctacttggat gtcttagttt
    22921 tcttagtgct tttgcttcta tctgtcttat tctctctcta gttacatcaa actcttttcc
    22981 aacttcctca agagttctgg ctctaccatc ctcaagacca aatctaagct ttaatacttt
    23041 ttgttctcta tcatttaatg aaccaagtac atcttctatt tgttctttta ataaagaata
    23101 tgctgctgcc tctgctgggg ctggagcatc atcatctgga ataaagtcac ctaaatggct
    23161 atcttcttct tctcctattg gtgtttctaa agacacaggg tcttgagcta ttttcattat
    23221 ttctcttact ttatcttctg tcatttccat ttcttttgca atttcttctg gttttggatc
    23281 tcttccaagt tcttgaagta gttgtcttga tactcttatt agcttattta tagtctctac
    23341 catatgaact ggtattctta tagttctagc ttggtctgca atagcacgtg ttatagcttg
    23401 tcttatccac catgttgcat aagtactaaa cttatatcct tttgtatagt caaatttttc
    23461 gactgctttt ataagaccta aatttccctc ttgtattaaa tccaagaaaa gcatacctct
    23521 tcctacatat ctttttgcta tacttacaac tagtcttaag tttgcttcaa ctaacctttg
    23581 ttttgctatc tcatcacctt cgtgcatcct tctagcaaat tctacttctt catgtggttt
    23641 aagtagagga atttttccta tttcttttaa gtacattctt acagggtcat ctatacttat
    23701 cccttttggt aaagataaat ccacagtttc tatttctatt gcagaagagt catcatgtga
    23761 aattgcctct gcatcttcat ctaagtggcc tatacttaaa tcgtcatcag caaccgaaaa
    23821 atctatatca gctttatagt tttttgtttc tgttatttct attcccaaat ttcctaaagt
    23881 ctcataaaga ttttctactt gatccttatc aagttcagtc tccgaaaaag cttccattat
    23941 ctctgcaagt gtcagagaac cttgcttttt tcctttttct attaatgtct tagcagtaac
    24001 cttttttaac tcttttttat tcgatttatt ttccacactc aaacctcctt tctctacaag
    24061 ctctttaaaa ttttatttat ctcaactatt tttaaagcaa tttccataac tctaccatct
    24121 acctctttca tatcattatt attttctaat tcttgttgtt ctctcaacaa gctattaatt
    24181 tgctcttcta atgagttttt tctgatattt gttattattc cttctatttc ttttgtgttc
    24241 tctaagttta tactattcaa agaaattgaa tttaattctt ttaagtattc ttcggatatg
    24301 tttaaacttt tcaatttgtc aatagttatt ttgtccaatt cttgattttt aatcatgtaa
    24361 tttaaaattt ctttactttc tttcaataaa aaatcacttt cttctacttt aagtaacgct
    24421 atttctctaa tttttttgtc ttctaacatt atttttatta gagtttcttc aactaattgt
    24481 tttccgtctt gtctgacttc gactttttca attacttttt cttctatttt cttttgattt
    24541 ttgttattat atggcttatt gtaattttta ccataaactt ctctctttat agactcaaca
    24601 ttaatatcta tttgattgct taaatacttt gtataaaaat caatttcaac aggacttgtc
    24661 aattgtttta taattttaga agcttctttt gcgaacttca ctcgttcttc atctttttga
    24721 atgttaaatt cttttttaag atgatctatt ttatatttta tgtagtgagt agaaacatcc
    24781 atagcttttt tataatcact aagtccgtat tttcttacaa attcatctgg gtctttagca
    24841 tcttttaagt ctaacacttt tacattgata cctaatttag taagtatatc tatagctctt
    24901 aaagtcgctt ttattcctgc ttcatcagaa tcataagata taatagcagt gtccgcatat
    24961 ctctttatta aaatgccttg ttgttcagtc aaggctgttc caagggttgc tactacattt
    25021 tttataccat actgataaag cgaaattaaa tccatatatc cttcaactaa gactatagtt
    25081 ttactttcta aattttttct agcaaaattt aatccgtata agttctgttt tttattaaaa
    25141 attaacgtgt ctggagaatt taagtatttt ggcaaagaat cgtctaatac tcttccccca
    25201 aatcctatta tatttcctct ataatcgaat ataggaaaca taactctatt tctaaattta
    25261 tcatagcaat tagttccatc tctatttttt gcaattagac cgcactccaa taagtcttgt
    25321 tttttatatc ctttactgat tagagtattc ataagtgaat tccatgaatc caaagaaaat
    25381 cctaaaccaa atttcttaat aattttatca tctagtcctc ttattcttaa atattcatac
    25441 ccaagatttt tgcttcccaa taagtttgaa agataaaatc tagctgcttc cgtatgaata
    25501 tcttgaaatt tcttagattt ttctatttta atcctagttt cttcattcat gttagtgtta
    25561 atttctatac cgcatttatt tgctaaaatc tttacagcat ccataaaatc caaattttcc
    25621 attttcatga caaaatttat tacgtctcca ccttcaccac aaccaaaaca cttataaatc
    25681 tgttttgatg tatttatata aaaagaaggt gtcttttcgc catgaaatgg acataatcct
    25741 ttgtaattag ctccagattg ttttatattc atatattcag atattatact tgctatatcg
    25801 catctagctt ttatttcttc aataatgtct tttatatcat tcatattatc accttctggt
    25861 gtaatttttt gtgttaacat ttctattcta cactatattc taaatttgtc aatcactcaa
    25921 tatacacaat tcgacttttt ttattgatat ccttcttatt aatcaaaaaa tttttatttt
    25981 ttatattaat tctatcaata ttaaatcttt atttatatta aataatctaa ctatcaaatt
    26041 ataacttata aatttacctt agcaacgatt acgaataaaa aagaggctct tgcctctttt
    26101 aataattatt aattgatttc attaaatctt caaattcatt aaagcaaaga gattgagcac
    26161 catctgacaa agcattttct gggtcaggat gtacttctac cattattcca tctgctccac
    26221 atgcaaaaga tgccagtgac ataggtttta ccaaatatct aactccagtg gcatggcttg
    26281 ggtctaccac aacaggaaga cctgtttcct tttgaataat gggtaccgca gctaaatcta
    26341 atgtatttct agtataatca ttatacgttc ttatacctct ctcacacatt ataatattac
    26401 tatttccttc tattgctata tattcagaag cacctatcca ttcagtaatt gtggatgcta
    26461 ttcctctttt taacataaca ggtttatttt gttttcccac ttcacttaaa agagtaaagt
    26521 tttgcatatt tcttgaccca atttgaatta tatcacttat actatacagt tcgtccatat
    26581 ctcttacatc cataagttct gtaataacag ccataccaac ttcgtcttta accgctttta
    26641 atatttcaag accttctttt tttaatcctt gaaatgaatt tggagatgtt cttggcttgt
    26701 atgctccacc tcttaaaata tttgctccct gactttttac aaatttagca gtctccaaaa
    26761 gttgctcata actctctatt gcacatggtc cagctattat aagtttatcc ttttttacag
    26821 ttatcttttt gttatttgta tttatctcta tctttgaatt attattaaat aggttttctt
    26881 tcataaccta caagctcctt tacttcttct ctttcttcag cagataggag gtttaaatgt
    26941 tcttttattg ttttttcatt tattataagt tcttcattta aatccataag tggtatcaaa
    27001 acgaaagctc tgtcttgaat ccttggatga ggtattgtta gtttttcatc agtagaaact
    27061 acatcattaa aaaatagtat gtctacatct atagttcttg gtccccaatg aactattctc
    27121 tctctgtgta gttctctctc tatttcttga caatattcta acaactcata aggttcaaac
    27181 tctgtttcta tttctacaca catatttaga aaatctgctt gttctgtata tccccatggt
    27241 tttgtttcgt ataaacttga ttcttttact ttatatatag aatcactatt tttcaaaagt
    27301 tcacatgctc ttgataaatt atcaaatcta tcacccatgt tagtacctat tcccaaataa
    27361 accttattca tctcttgttc tccttatctc aacgccaaaa tagtcataaa taccatttac
    27421 aggagcctca ggctttctaa ctctgaccat aactccattt ataagtataa atttatttaa
    27481 gacttcttca gcaatatttt ctgctagagc ctccaaaaga ttaaattgtt tattttcagt
    27541 aatatcttta acaacattgt aaacctcggc atagcttaca gatttattta tatcgtcact
    27601 aagacctgct tctcttgaat caatataaag ttccatgtcg acaaagaatt tctgacctag
    27661 aaaattttct tctttaagaa caccatgata tccatagaat cctagattac taagtaatat
    27721 tttatccatt atattatacc tactttctat atattgcatc agtcattcta gctgccatta
    27781 aattttcata aacatcatgt accctaacta tatctacgcc atctcttata ccaagaacag
    27841 tagtagctat tgtaccttct aatctttttt taggctcaac tggaagtact tttcctatta
    27901 ctgattttct ggatgtccca agcaaaatag gataccctaa atcttttaat tcatttaatc
    27961 ttctcaatac ttcaatattt tgttcaaaat ccttgccaaa tcctacacct gggtcaagta
    28021 ctattttttc tttttttaca ccacaactca ttgccatttc tatacttacc ttaaaaaagt
    28081 cttttattga ctccattata tctttgtcat agtttgtccc atcttgatta tgcattatac
    28141 acacttctgc atcatatttt cctattacag aagccatatt tttatctttt ctaagacccc
    28201 aaacatcatt taccatagtg acacctaatt ttaaggcttc ttctgcgaca tctgccttat
    28261 aagtatctat tgatattggt atatccaatt cttgtttaag tttttttatt acagggatta
    28321 ctcttcttag ttcttcttct gagtctacat agctatgacc tgggcgtgta gattctcccc
    28381 ctaggtctat tatatcagca ccttggtcaa ccatatcttt tgcatgttgt attgctatgt
    28441 ccaagttatt aaaatctcct ccatcagaaa aactatctgg agttacattt aatattccca
    28501 ttatataagt tcttttacca taatcaaaca caattaatga cactccctta aattaaattt
    28561 tatatctcaa taaatgatta tatctataat aaattgctac ttcatagtaa tcaaactcat
    28621 agcctcggct ctagacgcaa tgtccttttc aaatattcct ctcactgctg atgtaacagt
    28681 ttttgaacct ggttttttta cacctctcat tgtcatacac atatgttcag cttctactac
    28741 tactaagaca ccataaggtt gtaattcttc cattattata tctgcagcat ttttagtgat
    28801 tcgttcttgt aattgtggtc tttttgccaa tgtatctatt actctagcaa gtttagaaag
    28861 ccctgtaagt cttcctcctt ttggtaaata agcaatatga gcttttccaa agaaaggaac
    28921 tagatgatgc tcacaacatg aataaaatgg aatatctttt accaatacta attcttcatg
    28981 tttttcatct gcaaaaagaa cttttaaatg gtctcttggt tcttcactaa gaccagagaa
    29041 tatctcttca tacatcctag caactctatt tggagtctcg attagacctt ctctatctgg
    29101 gtcttcccca attgcctcta gtatttctct tactgcatgt tgtatctttt ctttatcaac
    29161 tttattcatt taaactcccc ccattgattt gtgactagaa gattagtttt ataacctctt
    29221 ttgtctcaat ttctttaaaa atataattat aaacatctaa aagtctttga tagtcataat
    29281 tttcacttac ttttgacact agttcaaaaa gctcattatt ttcttttttt aaaactttta
    29341 gcaatttttt gtcttttggt acccatttat catttatatt aaagtaccca attattatat
    29401 cttttaaata taaattcgtc aaaaaatgat attcaaaaac atcaaatttt tctttatttt
    29461 ttaaattcga tatttttgct ccaatatctt gctttataaa agacttttcc tctaatgaca
    29521 ttttactggg tccttctaag taaatatttc tacttatatc ttttataata tgtgatatat
    29581 tctccctatc aaacagtact ttagcatctt tcatttcttt cacaaagaaa tattccctat
    29641 ttgataataa tttttttaca ccatcttttg aaaagtaatt tacatcaaat tctattccct
    29701 tttttttaaa cagtattctc tcttgttttt ccccttcttt tacaaaaacg aacacatcaa
    29761 tatcgtttat ctctacatca tagagagtaa aatctacgtc tttactagaa cccactaaat
    29821 atacagccaa agtgttttgg ttttctatta ttttagtcat ctcttcattg tatacttcat
    29881 aaatacaacc tatcatcttt tgtctccaac aaattgttaa tattttaatt tattctaata
    29941 acttcactat tttatcaaaa acaacatttt ctttattaaa ttttattttg tcaatttctg
    30001 taacctttat agcgcccata agactatttg tgacaaaaac aaaatcaaaa ctactaattt
    30061 ctgatatatt aatttcattt tctatcaact ctatatgtaa ttcgtcacaa atctctataa
    30121 tccttttttt tattattcca tttaatatcg gtaatctatc actaggagtg tacactcttt
    30181 ctccttttat aaagaaaata ttgctcatag aacattctaa tattacacct tctgaattta
    30241 aaaatatacc atcattataa ccagtcttat ttgcgatatt tttggtatat atgttttcaa
    30301 aataattagt agtcttatgt ctatgaatta aactattccc cctaactatt ggagatatag
    30361 ctaatttaaa ccccttctca tacgtctctt gattatatgg aatatcccta atagatatat
    30421 tataaccttc atcaaaaaca gtaattctaa gtgctttatt aatgacattt tcttttttta
    30481 tatatgtaac aatttcattt atcaagaaat cttttttata gtttatatct aaatccaaac
    30541 aatttattga gcttagcatt ctttctatat ggatatctaa atctactgct attccatttt
    30601 taactctaat agtttcaaac aaaccaattc caaatttact taaactacta tcaaaaccta
    30661 cattattctt cataatatcc cctcaaagcc ttcattatgg atttagcttt atcaagtgtt
    30721 tcttgatatt cttcatccgg gtcagaatcc caagtcattc ctcctccaac ttgaaagtag
    30781 acttttttgt catttttgat tattgttcta attgctatat taaaatccat atcaccatta
    30841 aaacctatat aacctattga accagtatat acatttcttt gcgttggctc taattcgtct
    30901 ataatttcca ttgctctaat cttaggtgct cctgttatag acccccctgg aaaagttgct
    30961 tttattacat cagtagcatc tttatcatct tttaactcac ctacaactgt ggaaactaag
    31021 tgatttacat ttgcatatgg ttcaattaca aatagctctg gaactttaac acttccagtt
    31081 ttagatattc tcccaatatc atttctttct aaatctacta tcataagtag ttcagctcta
    31141 tctttttcac tatttctaag ttcttgttga agtcttaaat cttcttcttt atctttccct
    31201 cttggacgag taccttttat tggtctagtt tctattctct tattaacaca tcttataaat
    31261 ctttctggag aatttgataa tatatgtgta tcttcaaagt ttaagaatgc cccaaatggt
    31321 gctgggctaa atcttcttaa gtccctatac aattcaaagc ttgtaagttc tgtttctcca
    31381 ctgaaccttt gtgttaaatt agcttgatat atatctcctt gtcttatgta ctctctaaca
    31441 ctttgaactg catttttaaa ttcttccttt gtaaaattag atttaagtct tataggtgtt
    31501 acttcttttt cttcataaca tatgctatct ataccttttt tctcttcttt taatattctt
    31561 tgttctatat catctattat cttttcttct aattcgatat ctatgtttgg agtagctata
    31621 taagtttttt gcacaagatg gtctataact attacatgat tataaaatcc aaaatacata
    31681 tcaggcatct caatatcatc tacagcagtt ctaggtaaat tttctatgta attacctaaa
    31741 tcataagata aatatcccac agcacctcct acaaatggaa gattagtatc atttacaacc
    31801 ctatacttat gtagttcttc ttttaaaact tctaaaggat tttcatcagt atccttatac
    31861 tttaatacct taaatggttg gctacttata aaagaatatc ttcccaattt ttctttatcc
    31921 atagcactgt ctaatataaa actgtcatgt tcatttctaa aaatagtaaa tatctcaaaa
    31981 gaatttaatt tagtgtttat ctctcttatc atattacata tgtacccttt ctctagcttc
    32041 tgttataaaa tttttaagca tttcatgacc atactctgac aaaatagctt ctggatgaaa
    32101 ctgtactcct tctattaaat atttcttatg cctaatcccc attatttccc ctttatcagt
    32161 aattgctgta atttctaatt cttttggaac tgtattggaa tcaattatta gtgaatgata
    32221 cctagtgaca tttaaaggat ttttaagtcc cctaaaaact cctttattag tatggttgat
    32281 actatatact tttccatgca caggctgttg tgcttttata atatctcctc caaaaacatg
    32341 tccaatagtt tggtgaccta aacaaattcc tagtataggt tttttcccct taaaatgctc
    32401 aacaatatct atgcaaatac cagcttcttt tggagaacac ggtcctggag ataacacaat
    32461 tacctctgga tttagttctt caatgtcact tattttgatt tcattatttc tttttactac
    32521 aactgtttcc cctaattctt ctatatattg gactaggttg tatacaaaag aatcataatt
    32581 gtctatcata agaatcatac tgtaaatctc ccccctaaaa attattttat taactcagaa
    32641 actgttacaa atttaaaacc tttttcaatc atttttggaa taaacatatc taaagcttca
    32701 actgtatcat tagttgcata atcatgcaaa agtattatat ctccattttg tactttatta
    32761 attattttat ctgcaatgct attagctcct ggatttttcc aatctttcac atctacacct
    32821 gtccataaaa caactttata gtcatgcttc ttcgcaattt ctattaattc tttttctcta
    32881 taacttccaa atggtggtct aaataatgta ggtttttttc cagttacttc tttcaaaatg
    32941 tcttcacact taacaatctc ctcttctatc tgactagaac ttaaattact tatatcagga
    33001 tgattgaaag tatgatttcc tatctcgtga ccttctttac ttgctcttac taatggttct
    33061 ttataccatt ttgcatgttt tccagctata aaaaaagtag cttttacatt gtatttcttt
    33121 aacacatcta aaacttgact agtttcttta ggatgagggc catcatcaaa ggtaattgct
    33181 attagttttt tttcccttga accatgttta atcaaaacat tttcattagt atccagcatc
    33241 aaagcagaaa ctttgctaaa attagatatt aacactattc cacaaaacaa gactactgct
    33301 attaagcact tatatactgt ctttcttttc catgatgtca ttttttaact tcctttcaaa
    33361 tattttagtt atttataaaa attttgagtg taatatgttt tatagcttcc tccaaatatt
    33421 actccaactc caatattatt atagttcccc aaaatatttt ttctatgtcc ttcagagttc
    33481 atccaagctt catgcgcata tattgcatta agctgtcctg ctgctatgtt ttctccagct
    33541 gaagtatata ctataccttc tcttttcatt ctgtcaaatg gagtttcatt cttcttattt
    33601 acatggtcaa aaaaattatt atctctcata tcttcactat gttttcttga actcaaagtt
    33661 gcctgttcac tgtatctcaa tgagtttaac cctctttgat ttctaacact atttacaagg
    33721 tctataactt caagttcaaa acatttttta agttcttcgc tatcatctgg atataaattt
    33781 ttaaactcat cttcagtgct tttatcaatt attaaatatg aacaaactct attttcttca
    33841 tgaatatcgt agaaaattgt aatatactta ccttccttac ttattatatc atattgattt
    33901 tctgagttta ttatatatct ggtatttcct tttctcttat actttaatgg tgtaatctta
    33961 gttctcactg tttgtctatc ttgatttagt tttatatctt gattttcaca tgaattaata
    34021 gaattactat aaagtgctac tacttcatca ttttcaagtc caaccattgc aaattctttt
    34081 ccatattgat tatatacata ccaatcaaaa ccgtattcac tagtatcaat tctaccaggt
    34141 ttaccaatac ttgataaaac atagcttaaa ttgtctccta aacttatttt ttcaaaattc
    34201 tttatgtctg actctcctaa attctcccct tcagtattat ttgattgtaa attttgactt
    34261 ttttcttttt tatctttctt agaattatca tttatattta ttttgtctaa aaattcattc
    34321 catatttcac tttgaaaaaa cttttctttt aaatcatatc cttcttttaa taattcagga
    34381 ctataaaata aaacaataag tactactaac actaaagcaa ttaatctctt caatatatct
    34441 ctccttctta aaatcaatca aaatttttta atattgtatt tattaagtta atattgatag
    34501 tgtatactaa tcaatatcat tcatacctat ttacagtaag tattagaaaa aagctttatc
    34561 tactaattag ataaagctac agtctcaagg aaaatatttc atctaatgtt ctatcaaata
    34621 tagatggctt atcaaatttc caagtccctt cataaaccaa ttcattattt agataaaaag
    34681 ttccttgccc atgtttttta ccatgcataa attcacctat atacttttta cctttttccc
    34741 atatataagt tcccttacca tgaatcaaat tattcttcca ttctccttca taaagtactt
    34801 cccctttact attatagcaa ataccgtacc cttccatctt tccaccttta aattcacctg
    34861 catatataca tccatcactc cacttataaa taccttttcc atcttttttt ccatttttcc
    34921 aagttccaat atattcatct ttgctattat ataacatatt tccaataccg ctcatcatat
    34981 cttcctcaaa ataacctata tattcatttc catttgaaaa tacaaagttt ccttttccat
    35041 tttttttatc ttctttaaat tctcctatgt actccatagc tgttccttca tcgacaaaaa
    35101 aagtatatga gcctttcccc tccatctttc cctctaagaa tttccctaca tatacatccc
    35161 catttgaaaa tttatagatt ttattttttg gtttttttgt atcgacatca tctatatcgc
    35221 gtattgtttt tccttccaat tctaatactt cattttccgg agcaaaatcg ccccttttta
    35281 aaacacgttt cttcatgttc tttatacttc taatctcttt gtttacttca tttatatatt
    35341 tcaaataatt atcatacaaa ttaacatatt ctttagataa attcataatg ccattcctcc
    35401 aacgttattc ctagatattt tactaaatct attaaattta ttatatccta atagtggtca
    35461 tgttttctac atattgagta attttttcaa ttttaatttt aaaatgatat cttttaaagt
    35521 gatacacaaa tcattaataa aacctttatt ttcatatact aaatttaaat aagctgagag
    35581 atataatata caaatttaca ttataaactc ccagcttgta aaattttcta taaatctttt
    35641 gctttaaacc ctcttccttt tacttctgta acatcattta cagatataaa ggccttttta
    35701 tcatacttca aagctatatc ttttatcttt ggtatctcat ttgatgaaac tattgtatat
    35761 ataatcttct ttttttgatg agtgtatgca ccttctgcat ctaaaaaagt tacacctcta
    35821 actaaactct tcattatatc ttctgctatt aaatcgactt catttgacat taccataaca
    35881 gatttttggt aattcattgc atctttaata aaactcattg aatacgcatt tagaaataga
    35941 cctataagag tgtataatgc taaatccaac ccaaataaaa aacttcctgt aagtactatc
    36001 aagccattta ttgctaaagc agtgtctttc atttctatat tcttcttaat ttttagtatt
    36061 gcagctatta tatctgtacc accttgagaa gacctagttc taaatacaac tccgactcct
    36121 aaaccagata atataccacc atatacactt tgaagcaata tatcatgtat aggtataata
    36181 ttacttattt cctgagttgc tcctaataat aatgaaaata caatcatatt taccaaactt
    36241 gaaatacaaa actctttttc aaggtatatg aaccctaaga taaatattgg aatattgatt
    36301 aaaaatgtta ataaacctac atttatattt gtcaaatagt ttatagatgt tgctatacct
    36361 gtagcacctc cactaagtag atgtgctggt cttaagaacc catttattcc tatagatgtc
    36421 atcaacatcc caaatagcat tgttgaatat cttaaaactg gatgtttttg tagggtattc
    36481 ttaacttcac tcatttcttt ctcccttcaa tctttctaaa tctttagaat tttaaaagct
    36541 tactcttaca tatattattc ctgatgtaat agataatatt tcaaataaat tttttttata
    36601 atttcttgaa ctgtcatcta ttacatcatc tattatatta tctttttcat atatgtctag
    36661 tgcaatcttt tatattctaa tctttaaaaa cgttcaaatc atctcattta tgccttaaat
    36721 gaaacttttt ctagcataat atgatactgc ttattattta acttgcaata tagtaaattt
    36781 atagtatttt tttctaaata tctattttcg cagtaattta ttttatttta gcaaattcaa
    36841 gtttagtaat tgtctcttta taattaattc ctaaatgaaa tttttctttt tataatttca
    36901 gtacatgatt tttttatttc attatttgca tttttttcgt tcagttttta ttttaaattt
    36961 tttttatttt attttgtttt aagtattcaa ggtactcgtc ttaattttat tttttatata
    37021 aaattcatta taattggaat tagtattact gtaattgtac cagagactcc aatagaaaca
    37081 ccactcatag ctccttctat ttctccaagt tcaaaagcct ttgtagttcc taaagcatgt
    37141 gcagatgttc caagagcaat tcctttagca actggatgat ttatattccc cagtttgaat
    37201 actgtagaag caaccatacc accaaaaata cctgtaaaaa ttatggctac aactgttatt
    37261 gattctatac catttaaaga atttgctaaa gatattccca ttggtgttgt tatagatttt
    37321 ggaattagtg aatttattat acttacatct gaattagcta ttttgccaat tatcaataca
    37381 gatacaaaag aaatcactat accacaacct attcctacaa taatctcaaa catatgtttt
    37441 ttaaataatt caaattgttt ataaagtgga actgcaagta ctactgtgac aggacctaaa
    37501 aagaaattta gactatcagc tcctatcttg taattctcat aagggatttt agatatgctt
    37561 aaaaacaata ttattcctac gattgcaatt aataaagggt tacatatagg attacttgtt
    37621 tttcgttgaa tgtatcttcc taaattgaaa aaaactattg ttactattat accaaatact
    37681 ggtgtctgta atacattata catttttttg tccttcttcc ttctttttta tcattttctc
    37741 cacaataatt ccagtagtat acattactat tagtgtactt aaacatatga ctaataaaag
    37801 cactaataca ttttccctta ttaggtctaa tgactttata agtgatacac ctgctggaat
    37861 aaaaaatata gccatattat cgaggaaaaa tccacttaca ttttctatac tactcaagtc
    37921 taaaacttta aattgtagta gtagaaataa taatagcata ccaactatac tcccaggaat
    37981 aagtataatt ccttgtataa aggaacttat atactctcct attacccaga tagataaaat
    38041 tattaccaat tgattgaaaa ctttcatttt ccacctccaa aaattaaaga gattgcttca
    38101 aatcatatat ttgtagtttt atcaacaagc aaatatcttt ctttaaaata gaaagttata
    38161 atttgacaca atctcttttt aaatttattt ttattttaat ttaataaaat acatacttgg
    38221 ttgtttcaaa aaacttaact atataaacct atttttcact taaaatttct tttatttttg
    38281 aattgttata catactgtct ggaacttcac ttctcaaact ataaaaagtc tgttgtgcat
    38341 tttttaaatc gccattatca taataaagca tacccaactc gtaatatgaa tcatcataat
    38401 actggtcatt tttagtataa gtatttatat attttttgta atattttata gcttcatcct
    38461 tatttcctaa taatttattt gttattgcta attgatatat tgactcagaa atatattttt
    38521 tagttttacc agaagaaact acacttttaa aattatctat ggctttttca tattctttgt
    38581 ttttctttaa atttagagct tgataatata aatcttcctc atcagctaca gttatctctc
    38641 tttctggttc tttattttta acctcattta attccttatt cgttttctct aattttttac
    38701 tcgtttctcc taacttttta tcagtttcac ttaacttttc ctctttacta cttatttggt
    38761 tattcaagtt ttctattctc tttttgcttt gaatcaaata tatccccata cctattaaaa
    38821 cacagccaac tataatatat ggcaaaatac ttttctttct ttttactttt tttatttctt
    38881 cctttttact tgtatcttct ttttcttctt catttttttc aaatacagga ttatctttat
    38941 ctagctcttt aagtttgtct aaatatcctt ctctcttttt tacattacct agtttatcat
    39001 ataacaaaga taaaattaca tatggctcta ttagttcagg ttcttcttca attatgttct
    39061 ctaaaatatg tattgactca taatttaatt cttcatttat aaatcttata gaatgattgt
    39121 atcttcctaa aaaagtttta aaatcttcag atgaaattag atttacatat tttctagata
    39181 tatgattatt ttcataacat aaagcagtat aaaaactctc aaatgattta gcaaaatcac
    39241 attttaataa ttttaaaaga cctctaagat ttagtatatc aatgtcttta ggatttatct
    39301 ctagagcttt attaaccata ttaatggcac tagttatata atttttatat gctaaatcca
    39361 atgcattgtt atatctttta tatgattcta attttaactc cttagacgtt gtatctgact
    39421 ttgaatttaa agctccttgc aagtttaata gagtttcttc tttataattt gtagtcacat
    39481 aactctgttt taatgctttt aatgtttcat caccgtcttc ttcttctata ttaaaaaaag
    39541 tcaaatcact tttattatct tctatcaaag atatatctaa aatttttcta ggcatgcttt
    39601 ctatgtcttg ttttatatct ttaactaaaa taatactcac ctccactcta aaatatatat
    39661 ttaagataac atatttaata tgtcaagtca aaactataca tatttttcca ccattctcat
    39721 aataaaaaag gtaatcctct tcttttcata aaaaggttac ctctttttca atactattaa
    39781 cttttaatgt tgaatttttt tacttttcta tagagtgtag ctatacctat atttaatgcc
    39841 tcagctactt ttttcatacc ttctgtatta cttccatatt tctttatggc attttctata
    39901 gcttccctct caaggtcatc tatagtctta acttcttgtt tataaagttc atctagttta
    39961 aaagatatta aatcagacat actccttaca aaagcagtta actccttatc ttttgaaagt
    40021 atcttttctt tttgttcttc attaaacgca gctataccca agattccaag tatatgctct
    40081 cccattttaa cgggagtgca tatatcagct aactctttac aattttctct atttgcacat
    40141 ttattacagt tctcttctat ttctttattt ataacatagg attttccatc ttttaaaaca
    40201 agagagtaca ctgacttctc tgatattgtc tctcctattt tacctctgaa tattcctgta
    40261 cctgctatcc tagttaaatc ttttgtaact aaagtaacat ctacatccaa gacattagaa
    40321 atattctcgc aaatactttg aacgtaattc gttatattat ttaaattcaa agtaatccct
    40381 cctatttaga tttatatttt ttattactct acataaatcc tattcttgag aactttaagc
    40441 ttgcggttgt ggttgtgtct cattaggctt attatcagtt ggtggtgttt ctggtttttg
    40501 ttgattattt ggattttgat tgtttgtatt ttgattattc ttattttcgt ctgtatttgg
    40561 tttttcctct ggttttggtt ccatagtccc aactaatata ataccttcct tcttaggata
    40621 atatgaatta gctatttttt cagttttaat ttttttacca tttgcatcat taaatgttct
    40681 atatgttgat acagaatatg catttcttcc acttttgtct actttttctt tacctttagg
    40741 cattgtaggg tcattaactc ttttcattgt agtttgtgaa acgccatcta attttgttga
    40801 tattgttata ttttgtttat cttctgcact tccatatata ttgcaaacaa tttgatttcc
    40861 tgaaacgtaa ttttttatgt aaactggatg ctttagatta tttttaaata agaaatctat
    40921 tccactatct gcaacagttg catctcttcc ttttggaaca taacttgatg gtatagtatg
    40981 attttttaca ttaacaattt ctaaacctga atataacaca gaattgtata acgtagatga
    41041 aacttgacaa actcctccac cataatcttc ttccatctca ccttccatta ttacaggtgc
    41101 acttttatat ccattttcct tacttctttt ccctgtatgt ttattaaatg agaaagtttc
    41161 tcctggcata agtaatacat tacttatatt atctgttgct aattttatgt ttatacttct
    41221 atttatttga gaagttttaa atgttgttga atacgtacct aaatttgtat ctatattagc
    41281 aagttgctct ttttttactt ttgctggagt agcttttact actaactcat ttttatagtt
    41341 acctttttta gtttgattat caaaattctc catactctta gaaatatcca ttttaagacc
    41401 atcagaatct ggtataacct tgactttttc accacttata tcaagtgtag catcttttac
    41461 gtctacatct atttctttag ctatcttttc catttctgct tttaatttat ttttatcata
    41521 atttatcgca actactaagt tgcttttttt gccaaaattt gctgaaatag tttttgatag
    41581 attgccaaag aaagcactct ttctatttaa attatatgcg ttttctactg ttttatccag
    41641 gtcagtagat aaatttaaat ctttgctctt tactttccaa cttttatcat tgtaattaaa
    41701 atcaacattc tctaatttgt attttttagc taattcctgt tttgcttgcg atttagttaa
    41761 ttttccaata tttactccat taacatatgt gttttttgct attttctccc ctttaaattg
    41821 catgcacatt atgcctataa aaattattac tactaatgct acagctccaa tagctataac
    41881 tagctttttg ttcacattac tcatcccata cttctcctta caataaaatt atttatatat
    41941 ccctattatt atattactac ataatgcgcc ttaaaacccc aaaaactttt taattatata
    42001 ttaatttaat attattaatt aatattcatt tagtccattt tctgatatgg catatatttt
    42061 ttcattgtaa atattaatag cataaacttc tttgcctcta aataagcctt tatttactac
    42121 agcataataa taaatatttt tatattttcc aaacaattct tctaaactct ttttactttt
    42181 tacatatcta tctgctttct ctatatctaa tatctcaatc tttttatact tattttcttt
    42241 ttcatcaaga gtattagtta acacaaactt agctttatct tttgtaatac caaattgata
    42301 acttccatct tctaatattc taaagtatcc atcaccataa tctactttgt aatctttttt
    42361 accatcttta tatgaatata aatctttagt ttttgcactt accaaaactt catttattaa
    42421 tttatcatac tcatatactt cagcataata gtatttatta ttttttatca tttgtggaag
    42481 tgaatttata gatagatttg ccttgtcttt gccacttttt ttagctatta catttataat
    42541 gtcatcagat gttaaagtat acaatgataa attcttttta tatttttttt ctaaactctg
    42601 taccttttca tcatctgaat aatcgtcttt taaatactca atatattgta atgctctttc
    42661 ataatctcca gaagtatttg cctcttcagc tctacttata tagtaatcat acatttcttc
    42721 aatacattgt tctttcccat tttgagccat actgtaatac ttttcatctt cttttaatac
    42781 tttattatac ttttcaatag cttcataata cttatatgct ttttggtctt ttactgctga
    42841 gtcataaaca tctctagact ctttgattgt ttctatattc tgtttgtata catctaggtt
    42901 actacccata ccatttaata ttgaaacagt acttatgtag ttaatggcaa tatcataagt
    42961 agtattttct tctttataca tttctctaac tctatcagct tgagctaata agtcatccac
    43021 atctatactt attttgttta attctttcac ggtatttatt aatccaataa aactttcttg
    43081 acttatgtct ccatctaaat atttatcacc actatttaat agcaatttat ttattttttt
    43141 agaaacagac ttattaaacc tttccatttt tgaatcagaa aacttttttt ctatacttgt
    43201 gtagtaatca acagctatag aatactgtct tttctctata atctttaaca actcattagc
    43261 ttcatttcct acactaaact cgttactact taagaaaaat actattatca aagcaattac
    43321 acctaatgtt gataatgaaa tcgcaactat agatttcctt ttttcatcaa taaaatctac
    43381 agccttctca aataaatctc taaaattcat tatagttccc tctccattta ataatcttta
    43441 tattttatgg taaactaaat ttcttattgg ttattatatc ataattcttg aaaaaaaata
    43501 aaaacacaag ttaaatcgtg tttttatttt acctatcaat cattatttta tacataaata
    43561 tttcctttac ataaattatg atatctttct ttttatatcc ctagaatgac tcttttgttt
    43621 tttattatta ctgacagttt tcgttcttgt cttgtgtata gctttgctat caaatatttt
    43681 tctagcattg cataaaacaa ttccaattgt taaacaaact gtaagcacac ttcccatcaa
    43741 aaagtaataa tccaatccta tatgtactcc cataactgaa tttattaaca ctaatgcaaa
    43801 agtcattgca aaacatatca tcatggagat aactaaaatt cttagaattt ttttcaatcc
    43861 actcacctcg tataatcact aaaattgaca ctacccctta tccattacta taccaaatta
    43921 gtaaaaaaat agcaattact attgtcattt atattaggaa aatattaaat ctttttaata
    43981 aatatctttt tttgacataa ggattactta acttttaata aaatagtaac actaatatgt
    44041 attaattaat agaataaaat aaatgataat ttattgaggg gtgatttaca tgccaaattt
    44101 gccaagttta gggtcaaagg ctcctgattt taaagccaat acaacaaatg gtcctattag
    44161 actctctgac tataagggta attggattgt tttattttca catcctggtg attttacacc
    44221 agtttgtact acagaatttt tatgttttgc taaatattat gacgaattta aaaaaagaaa
    44281 tacagaacta attggtctaa gtgttgatag taacagttca catttagctt ggatgtataa
    44341 tatttcttta cttacaggtg tagaaattcc atttcctatt atagaagata gagatatgag
    44401 aattgccaag ttatacggca tgatatcaaa accaatgagt gatacatcaa ctgttcgctc
    44461 tgtatttatt atagataata atcaaattct aagaacgatt ctttattatc cactaactac
    44521 aggaagaaat attccagaaa tacttagaat agtagatgca cttcagacta gtgatagaga
    44581 taatatagtt actcctgcaa actggtttcc tggaatgcca gtgattttac cttatcctaa
    44641 aaactataag gaattaaaaa atagagttaa cagttgtaat aagaaatatt catgtatgga
    44701 ctggtactta tgttttgtac cagataatta taatgatgaa gaagtgagca agaaaattga
    44761 taatacttgt agctggaaaa aagaacatac taaaaacatt gaaaatgaat gtaactgtga
    44821 acatgaacat catgactacc tgaacaaagc tcttgactgt aaacaagaac acaagactga
    44881 tattaaagat gattgcaacc atgagaaaaa acatactaaa aatactaaca aagttcacaa
    44941 ctccaaacaa gataagttta aagataagtc ttgtgatgaa atgaatttta actatgataa
    45001 agatgaatct tgtgacaaaa taaattctag ctataacaaa gaagatagta gttatgaaga
    45061 tttctataaa cataattata aaaactacga ttatactagt gaaaaaaata ctaaaaaaat
    45121 agctatgaag actttaaaag attcaaaaaa actagctaga ccacaaataa cagacccata
    45181 caatccaata gttgaaaatg caaactgtcc agatataaat ccaattgtag cagaatatgt
    45241 tcttggaaat ccaactaatg tagatgctca actattagat gcagttatat ttgcttttgc
    45301 tgagatagac caatctggaa atttgtttat tccttatcct agatttttaa atcaattact
    45361 tgctcttaaa ggtgaaaaac ctagcttaaa agtaattgta gctattggag gttggggagc
    45421 tgaaggattc tctgatgcag cattaacacc tacatccaga tataattttg caagacaggt
    45481 caatcagatg ataaatgaat atgctttaga tggaatagat atagactggg aatatcctgg
    45541 aagtagtgca tctggaataa catcaagacc tcaagataga gaaaatttta cactcttact
    45601 aactgccata agagatgtta taggagatga taaatggctt agtgtagctg gaacaggaga
    45661 tagaggatat ataaattcaa gtgctgaaat agataaaata gctcctataa tagattattt
    45721 taatcttatg agttatgatt ttacagcagg tgaaacaggc ccaaatggta gaaaacatca
    45781 agcaaatctt tttgattcag acttatcttt gccaggatat agtgttgatg caatggtgag
    45841 aaatcttgag aatgctggaa tgccttctga aaaaatcctt cttggtatac cattttatgg
    45901 aagattaggt gctactataa caagaactta tgatgagctt agaagggatt atataaataa
    45961 aaatggatat gaatatagat ttgataatac tgctcaagtt ccgtatttag ttaaggatgg
    46021 agattttgca atgtcatatg atgatgcttt atcaatattc ttaaaaactc aatatgttct
    46081 tagaaattgt ctaggtggtg tattctcatg gacatcaact tatgaccaag caaatatact
    46141 ggctagaacc atgtctattg gtataaatga ccctgaggta ttaaaagaag aacttgaagg
    46201 tatttatggg caattctaat agaatgtagg aaaattttat agttatataa aaagcagatt
    46261 ttattatatt aaataagttg tacctaatta atactattat taattgttta taaattagat
    46321 acttattttt tcataataca atctgctttt ttaatcaaaa tttatttaat tataaattgt
    46381 ttaattacaa gttgtttaat tacaagttgt ttaattataa agtaggaatt tagatatact
    46441 cttataccat gtaattatgt atataaatat ttttttatta tacaccaata gcttttttgg
    46501 ctaatttatc agcttcctca ttatacttat ttccagaatg agctttaacc ttcgtaaatt
    46561 ttacattaag cttattctta atactgtcat agaaattttt atatgctata gttccttctt
    46621 tatttgtttt ccaaactcca gtacaccact tttcaatccc ttcatagtca aagtataaaa
    46681 tcaagttttg aatattattg tcaatacagt actgcattgc aattttagaa gcttctatct
    46741 ctccggctac atttctcata cttactaaag tttcctcttt tcctttttca ctataagttt
    46801 ttataacttc attatttttt aaaatcacaa cacccgaacc atacattttt attgaatgtt
    46861 cataacttcc atctacatat gcttctataa actccttatc ttcttgaaaa ctcaattttt
    46921 ctgacccata aatatattct ttagcctctt gaaaagtaga aaaactctta tattcagctc
    46981 ctgagaatcc atttacttgt tttttgcact catcccatgt attatataca cctatatttt
    47041 tacctttttt cacagcataa aacttttgtt ttgccaaata tatcactccc actcttaatt
    47101 tttatcttct tctctcataa atatatctaa atttataaga tagtttctat caaaatattt
    47161 tactttcata tcttttttta taatatcaat atggtacact tctcctttta cagctctttg
    47221 tcttatattt tcatttatat ctttttttat aatatcctta tcaacatcat tgttatccat
    47281 tttagcatca acattcaacg cttttatagt atcaaatata acagatttta gtattaaact
    47341 tctagattta tttaaatgtc gccttatata agtataaaac tcattgctat taacctttga
    47401 aattttacaa tcgtgtaaat ctttctctaa tctctttaac acacttatat tagttcctac
    47461 atcaaaaatc acaaaacatc catagtcata tttgcttctt aaaattctcc ccatagcttg
    47521 ctttaccttt atggtcattt gaggataatt tatagtatag tagtctatac catacttctt
    47581 cattatagta aaatacaaag ggtctttagg atttagattc ggaagcttgt ctaaagttac
    47641 acaaaccaaa ccatcccctg gaatgtctac gccttcaaaa catccttttg agccaagtac
    47701 tacacatttt ctatttaaat cattaaggtg tctaatgccc tttttatcag cataaatttc
    47761 taagttaaaa ctatgtaagt attttttcaa tatttcataa gttttttctt gtctatcttt
    47821 actattaaaa agagctaatg tatgaccttc tgtaatcttt gatatattag atataatttt
    47881 actcatctca tttggaaatt cagaatttct gtactcacaa atatcagaaa atccaactac
    47941 agagactcta tttttataat caaaaattgg ttctataatc ttttccacat tcttaactct
    48001 gtctattcct aatgtatttt taaaataaga catactttca gataaactca aagttgctga
    48061 taaaaatatt cctttttcta attgagataa aatattttct tcaaataaat ctgctatttt
    48121 taatggcact actcgaaatt caaagtcatt ataattctta tcaatttcaa ctatccttgc
    48181 ataatcatcc ttttcatcat actctaaaaa gatttcaaat ataatcttta tatcctctaa
    48241 atctcttgtc tttgcctttc caaatttata tatatctgct tctttatcta tactatcatc
    48301 atcaatattc ctataaatta ttattattat tgaaactaaa ttttttatta ttttctcaca
    48361 actcaacttt atcttttcac tataggctct ataacttatt tcagtatcta taccaccctt
    48421 ttttagtttt cccacaattt catctatttg caaattcagt tcccatctta aattataatt
    48481 acttacatta ttatattcat tacaattacc aaaatttaat attgaatcta tctcttcaat
    48541 aatgagattt atgcttctag caattttttg tttatcttct cttccaattt ttaatacatt
    48601 ataaaattta tcaaaagcct ttatttttct catatttcta ctatattttt tatatataaa
    48661 gctactattt tgaataaact cgtaaggata aatttcttgt aataaatacc ttaaagactt
    48721 tgaatttata atgcttgaaa agaaatcata tcctttttca gtcaaattat gtgcttcatc
    48781 tactattata ttttctaaag gtttttcatc cttatacggc catttagcta atagtgaatg
    48841 atttactact gttatatgtt cttcttttaa ttcttctatt ctgtttttat ataaacaatc
    48901 ttttttgcat tttttaggtc tgcacatatt tgggtcacaa cttacatttc ttaagtgagt
    48961 acttatttcc ttgaagttgt caaacactaa ataatttatt tcttctatat ctccatactt
    49021 gccaccttca actaatctct tcaaaaatat taaagataaa atttctcctt tagtagggtt
    49081 ttgagactca taatcatcta tatatgcttc cagtctatct atacaaatat agttattttt
    49141 tccctttata tatccgtaac ttacttttcc atttagaccc aaagaatcta acacatttgg
    49201 aatatcttta tttattaatt gtatctgcaa ttcctttgtg tcagtagaaa ttaataatct
    49261 ttttttatta attctagcct ctaatattgc aggcaataaa tatcctacac ttttacctat
    49321 accagttggt gcctctatac atgcaatttt ttcttcatct tcgctatttc taaacaactc
    49381 tcttattgtt ttagtaagct catattgacc aggtctatat tcatatgtaa atccttcttt
    49441 attctcccaa atagttttgt attttaataa ttcttcataa tttttttctt cttcatttaa
    49501 aatttttaag gtttctcttt ccttcttctt ttcttctttt gagtcaaata tattatattc
    49561 tttttttata tttatattat ttgataaatc ataatttgcc tcttctaaaa atttgctcca
    49621 ttcccatgtt ggaagattaa attttttaag atatgaattt attttaaaac tcaatggctc
    49681 caaagtagtt ttttcatagt tattaaattt tactaaaagg caatttacaa tatttattat
    49741 atcaatagca tctgataaag ctctattttc cactttagaa ttacaatttg ttaaagtatt
    49801 ttttaagtat tctaagctat aatctttatg atatggttct aaaataactg ctaattcaat
    49861 taaatcaata aatttgtttt ttaactttgg catatgatac tcaaaaaact tttttttcag
    49921 acttaaatca tgacatataa tgaaactatc ttctacaaaa tttacaagtc tatcttccac
    49981 cagttctatt tcattagcta tttctaaatc atttttatct aaatttttac atactgagaa
    50041 tacttctata ggtacctcaa atttgttttt tattaatgat tcataggtat gtattttcca
    50101 gtctttaacc ttgacagctc caacttctaa aatttctgaa tttaaacaat ctaatccact
    50161 aacttctata tctaaaaaaa caacatcatt caatacactt attatattat ccatttttat
    50221 atcccttcaa gttaaaccag cctaacttat ttacaatcat ttctctttat attttatcat
    50281 ttatatacac tttatttaag ataattaata tcaactttat atcttacagt aaacttcttt
    50341 catttattat ttgtatatgt gaatattttt aactataaat attcaaacta tattataaga
    50401 aatatacttt aaattttaaa taagaggtga aattgtgaaa aaagatagcc taaaaaaata
    50461 cattatgata ggagcatttg ctctaatact ttttggaatt gctagtataa attttaaatc
    50521 attagataaa acaaaaactc aaatttctag tccaacactc gacacacatg aatatgattg
    50581 gtattttaac cctagggaag atggtaaaca gccatcacct ataaaagaag ctgatttttt
    50641 taaaaaatac ggagcttatt atgtaggaaa tcctaatgaa aaagttatat atctatcatt
    50701 tgatgctgga tatgaaagtg gaaacactcc aaaattatta gatacgctta aaaaacacaa
    50761 tgctaaagct caatttttcg tagttgaaag ttatataaaa agcaatccag agttaataaa
    50821 gcgtatggaa aaagaaggac atttagtttg taatcattca aaaagccatc catcaatggc
    50881 aggtattaca gattttgaaa aattcaaaga agaaattaca agtgtagaaa aagcatacaa
    50941 agatgttact ggaaaagaaa tgcctaagta ttttagacct ccaatgggta aatttagcga
    51001 acaatcttta aaatatacac aagatttagg atataaatca atattttgga gttttgctta
    51061 tgtagattgg tatgaaaaga aacaacctac tcatgaattt gccaaaaaca aaatttattc
    51121 aagaactcat cctggtgcaa tagttttact gcatccaaat tcatcaacaa atactgagat
    51181 attagatgag gttcttacgc attgggaaaa agaaggttat aaacttaaaa cacttgatta
    51241 tttaaataat aaaaaatagt atcataaatt taaaaaaact ctatcatgaa ttatagtaaa
    51301 aatatagtta tttataaata ctttaattct cgatagagtt ttttattttt tatagctatt
    51361 taaaaagaat tgtccattca tttatctctt tgtatcctat attatgatat attttacctg
    51421 ctacatcatt attataaaaa agacatggaa cttttccttc taacaacaat tctttactca
    51481 aattgtaaac catataactt gcaagtcctt ttcccctata tcttttatct gtacttacag
    51541 agaccaccat tgccaaaaaa ctagtttcca taccagttga cacagtgcta atcatagtat
    51601 catcatttct aataaaatat gctcttacat ttccctcttt taaatgaact tttctttctt
    51661 ctatgtaatt tttacttact ttatagtctg acctatttaa aagttttcct atttcatcaa
    51721 tatctttttc ctgagcattt tctattttat actccttcat gttggaaaaa tctatttctt
    51781 ttatttcttt taatacacaa aatttattat ctagtttttc ataaaaatct ttgtatttac
    51841 ttattagtct atctattaca catttttttc cacttatgct ttctacatct aaatctttta
    51901 tatgattctt catttctcct atattaaaat tatttttaaa actataaatt ataagtgttg
    51961 acttattttt taatattata gaggttatat ttccatcttc tacctgagac catatatctt
    52021 gatattgaga gctaaatcca taattctcaa cgtatccaat tatgaataaa tttaaacttt
    52081 cttcatgtga tacatattgc caaaattctt tttctaaagt attatctatt tttacaatca
    52141 agcagatatc ctccttctat gttaataatg atatacctta taatttatag tatcatttaa
    52201 aatcattata ttctaccaaa tattctaaat ttttaaatat ctatgcaata tgttcgtatt
    52261 tatttttata ttctgttact gcttcactcc aagcttcttc tactgaaatc cccctcttat
    52321 ttgcaatttt ttgtgctaat tctattattt tcatagcttt tcttgatata ttcatatata
    52381 ttcctcctct aaattatcat aagttatttt aaagttaaat ttctatagtc atgttatata
    52441 tttatttact tatatcttac taattattta tgtactaaat atgaactaca atagaagaaa
    52501 tttagaactt aataaaacat aaaatttata tacagttcat atatatttgt catatcacaa
    52561 gacaagtgat ataatttatt atcattttac tgggggtaat atgctatgaa aataagagct
    52621 gaatatacat gtcctcttga aattgtacat gacataatca aaggaaaatg gaaaactatc
    52681 cttatatttg aattaagaca atctaataaa acattttctg aattgcaaca taatatttat
    52741 ggaataaatc aaaaaatgtt gattcaacaa ttaaaagagt tacgtgattt tgggattatt
    52801 gataaaataa gttctgctgg atatccatta catgttgaat attttcttac taatcgtggt
    52861 aaaaaaatgt taaaagcagt ggaaataatg caagaaatag gaatcgaata tatgcttgaa
    52921 catggtcaac aagaaattct agataataaa ggaatttgct acaataagta atcctataca
    52981 taccaaaaag taagtaattt acatttttta caatagtatt ataataaaaa taaataggag
    53041 gtataatcat gtctatagct ttaatattga ttgatattca aaatgattat tttaaaaatg
    53101 gaaaatgtga attatttcaa tcagaagaaa ctgctgaaaa tgctaaaaaa atactactgt
    53161 tttttagaaa aaacaattta ccagttattc atatacagca tatatcaaca gataaaacag
    53221 cttctttttt tcttcctaat actcatggtt cagaaataaa taaaattgtt tttccactag
    53281 ataatgaaga aattataatt aaacacacac cagatagttt ctttgaaaca gatttgcaaa
    53341 gtatccttga gaaaaaaaat attactaatc tagtaatctg tggaatgatg agccatatgt
    53401 gtattgatac aagtgtaaga acagcaaaga aacttcgata cgacataact ctaataagcg
    53461 atgcatgtac aacgaagaat ttatcttgga ataatataga aattaatgca aatactgtac
    53521 atcaagtatt catggcatca ctacagaaca gttttgcaga tgtaaaaaac acagataaat
    53581 ttttatctaa ctatcacaaa taaaaacata cactatgtta aaagagcaag tttgatttat
    53641 ttaagcttgc tctttaatca tttaaaatta gatgctttct atcattttta tatacataat
    53701 atacgtttat acattttata ttcatatata actattatat aaattttaaa ctttactata
    53761 ttcacataaa aa
//
LOCUS       DAEORZ010000004        53186 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEORZ010000004 DAEORZ010000000
VERSION     DAEORZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53186
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.004"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            62..796
                     /locus_tag="KRH39_000182"
     CDS             62..796
                     /locus_tag="KRH39_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG8510114.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRRSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPGMKDELRRPMMLLVSAKKR"
     gene            complement(939..1430)
                     /locus_tag="KRH39_000183"
     CDS             complement(939..1430)
                     /locus_tag="KRH39_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG8510115.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1779..2162
                     /locus_tag="KRH39_000184"
     CDS             1779..2162
                     /locus_tag="KRH39_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510116.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            2670..3053
                     /locus_tag="KRH39_000185"
     CDS             2670..3053
                     /locus_tag="KRH39_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG8510117.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            3055..3405
                     /locus_tag="KRH39_000186"
     CDS             3055..3405
                     /locus_tag="KRH39_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510118.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(3506..3964)
                     /locus_tag="KRH39_000187"
     CDS             complement(3506..3964)
                     /locus_tag="KRH39_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510119.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLKEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(4009..4797)
                     /locus_tag="KRH39_000188"
     CDS             complement(4009..4797)
                     /locus_tag="KRH39_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8510120.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(4936..5373)
                     /locus_tag="KRH39_000189"
     CDS             complement(4936..5373)
                     /locus_tag="KRH39_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HBG8510121.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            6195..6704
                     /locus_tag="KRH39_000190"
     CDS             6195..6704
                     /locus_tag="KRH39_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8510122.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGRLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     RIFLTRKKI"
     gene            7109..7645
                     /locus_tag="KRH39_000191"
     CDS             7109..7645
                     /locus_tag="KRH39_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG8510123.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKR"
     gene            complement(8078..8878)
                     /locus_tag="KRH39_000192"
     CDS             complement(8078..8878)
                     /locus_tag="KRH39_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8510124.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(9073..10671)
                     /locus_tag="KRH39_000193"
     CDS             complement(9073..10671)
                     /locus_tag="KRH39_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG8510125.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIINAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGVEVDNSVSLSN"
     gene            10999..11175
                     /locus_tag="KRH39_000194"
     CDS             10999..11175
                     /locus_tag="KRH39_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510126.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNDAF
                     STLEKELNISKANT"
     gene            11349..12479
                     /locus_tag="KRH39_000195"
     CDS             11349..12479
                     /locus_tag="KRH39_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG8510127.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            12720..13070
                     /locus_tag="KRH39_000196"
     CDS             12720..13070
                     /locus_tag="KRH39_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG8510128.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            13078..13527
                     /locus_tag="KRH39_000197"
     CDS             13078..13527
                     /locus_tag="KRH39_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBG8510129.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            13629..14204
                     /locus_tag="KRH39_000198"
     CDS             13629..14204
                     /locus_tag="KRH39_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG8510130.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            14197..15606
                     /locus_tag="KRH39_000199"
     CDS             14197..15606
                     /locus_tag="KRH39_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBG8510131.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            15790..17223
                     /gene="eutA"
                     /locus_tag="KRH39_000200"
     CDS             15790..17223
                     /gene="eutA"
                     /locus_tag="KRH39_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBG8510132.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDETIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            17241..18605
                     /locus_tag="KRH39_000201"
     CDS             17241..18605
                     /locus_tag="KRH39_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBG8510133.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            18618..19499
                     /gene="eutC"
                     /locus_tag="KRH39_000202"
     CDS             18618..19499
                     /gene="eutC"
                     /locus_tag="KRH39_000202"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBG8510134.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTILDKKASGIDLK"
     gene            19519..20172
                     /gene="eutL"
                     /locus_tag="KRH39_000203"
     CDS             19519..20172
                     /gene="eutL"
                     /locus_tag="KRH39_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBG8510135.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            20183..20884
                     /locus_tag="KRH39_000204"
     CDS             20183..20884
                     /locus_tag="KRH39_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG8510136.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            20887..22356
                     /locus_tag="KRH39_000205"
     CDS             20887..22356
                     /locus_tag="KRH39_000205"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBG8510137.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            22452..22739
                     /gene="eutM"
                     /locus_tag="KRH39_000206"
     CDS             22452..22739
                     /gene="eutM"
                     /locus_tag="KRH39_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBG8510138.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            22866..23627
                     /locus_tag="KRH39_000207"
     CDS             22866..23627
                     /locus_tag="KRH39_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HBG8510139.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            23640..24269
                     /locus_tag="KRH39_000208"
     CDS             23640..24269
                     /locus_tag="KRH39_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG8510140.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            24311..25036
                     /locus_tag="KRH39_000209"
     CDS             24311..25036
                     /locus_tag="KRH39_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBG8510141.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEGTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            25049..25321
                     /locus_tag="KRH39_000210"
     CDS             25049..25321
                     /locus_tag="KRH39_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBG8510142.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            25314..25865
                     /locus_tag="KRH39_000211"
     CDS             25314..25865
                     /locus_tag="KRH39_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG8510143.1"
                     /translation="MNKSIGAIEFKSIAKGIKVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            25877..26968
                     /gene="eutH"
                     /locus_tag="KRH39_000212"
     CDS             25877..26968
                     /gene="eutH"
                     /locus_tag="KRH39_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG8510144.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     ALAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            26970..27443
                     /locus_tag="KRH39_000213"
     CDS             26970..27443
                     /locus_tag="KRH39_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBG8510145.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(27563..28087)
                     /locus_tag="KRH39_000214"
                     /pseudo
     CDS             complement(27563..28087)
                     /locus_tag="KRH39_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(28097..28702)
                     /locus_tag="KRH39_000215"
     CDS             complement(28097..28702)
                     /locus_tag="KRH39_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8510146.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQHKR"
     gene            28872..30230
                     /locus_tag="KRH39_000216"
     CDS             28872..30230
                     /locus_tag="KRH39_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510147.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            30223..30855
                     /locus_tag="KRH39_000217"
     CDS             30223..30855
                     /locus_tag="KRH39_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG8510148.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            31184..32341
                     /locus_tag="KRH39_000218"
     CDS             31184..32341
                     /locus_tag="KRH39_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8510149.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(32488..33123)
                     /gene="lexA"
                     /locus_tag="KRH39_000219"
     CDS             complement(32488..33123)
                     /gene="lexA"
                     /locus_tag="KRH39_000219"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBG8510150.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(33227..34354)
                     /locus_tag="KRH39_000220"
     CDS             complement(33227..34354)
                     /locus_tag="KRH39_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG8510151.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPAFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(34676..35521)
                     /locus_tag="KRH39_000221"
     CDS             complement(34676..35521)
                     /locus_tag="KRH39_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510152.1"
                     /translation="MIYIVEILKISIINIFYFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMTIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(35551..35922)
                     /locus_tag="KRH39_000222"
     CDS             complement(35551..35922)
                     /locus_tag="KRH39_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG8510153.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILNYNGYEVE"
     gene            complement(36075..36335)
                     /locus_tag="KRH39_000223"
     CDS             complement(36075..36335)
                     /locus_tag="KRH39_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBG8510154.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(36524..37468)
                     /locus_tag="KRH39_000224"
     CDS             complement(36524..37468)
                     /locus_tag="KRH39_000224"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBG8510155.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSIVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYNKFRNIGKCL"
     gene            complement(37489..38343)
                     /locus_tag="KRH39_000225"
     CDS             complement(37489..38343)
                     /locus_tag="KRH39_000225"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBG8510156.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(38358..39719)
                     /gene="accC"
                     /locus_tag="KRH39_000226"
     CDS             complement(38358..39719)
                     /gene="accC"
                     /locus_tag="KRH39_000226"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBG8510157.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEENNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(39735..40187)
                     /gene="accB"
                     /locus_tag="KRH39_000227"
     CDS             complement(39735..40187)
                     /gene="accB"
                     /locus_tag="KRH39_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBG8510158.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(40458..40904)
                     /locus_tag="KRH39_000228"
     CDS             complement(40458..40904)
                     /locus_tag="KRH39_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510159.1"
                     /translation="MESKFFNWYTQSLGGTLGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILTTVVFGF
                     IGARVYFIIPAILITFNLYSDILIAKLKCILCKNKENDTDLKESEV"
     gene            41189..41467
                     /locus_tag="KRH39_000229"
     CDS             41189..41467
                     /locus_tag="KRH39_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510160.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(41596..42342)
                     /locus_tag="KRH39_000230"
     CDS             complement(41596..42342)
                     /locus_tag="KRH39_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510161.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDGVVNSLSNNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGENIDLRKIITKKIDSYYKYWEVTDNLPKDLDFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(42471..43115)
                     /gene="trmB"
                     /locus_tag="KRH39_000231"
     CDS             complement(42471..43115)
                     /gene="trmB"
                     /locus_tag="KRH39_000231"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBG8510162.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLAMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(43253..43573)
                     /locus_tag="KRH39_000232"
     CDS             complement(43253..43573)
                     /locus_tag="KRH39_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBG8510163.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            complement(44020..44445)
                     /locus_tag="KRH39_000233"
     CDS             complement(44020..44445)
                     /locus_tag="KRH39_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG8510164.1"
                     /translation="MHDELKIKMYSFTMDCKDPYELAKFYAALLNWEIAFYNEEYACV
                     GAPGTNQGTYPGITFQRNPEYKPPVWPEKPEAQQQMAHMDFAVNDLEEAVQYAIRCGA
                     TIAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(44604..45059)
                     /locus_tag="KRH39_000234"
     CDS             complement(44604..45059)
                     /locus_tag="KRH39_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG8510165.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYELPIYQQSNNFQLYFEVDDIKQWESKINRIENIDFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFIIQFQ"
     gene            complement(45097..45930)
                     /locus_tag="KRH39_000235"
     CDS             complement(45097..45930)
                     /locus_tag="KRH39_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8510166.1"
                     /translation="MLKKESIRFINKNRSLLVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGGIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLKIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            complement(46204..46890)
                     /locus_tag="KRH39_000236"
     CDS             complement(46204..46890)
                     /locus_tag="KRH39_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510167.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILYIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLDKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            complement(46905..49382)
                     /locus_tag="KRH39_000237"
     CDS             complement(46905..49382)
                     /locus_tag="KRH39_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG8510168.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMSGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLIAADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRHTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYTVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTHILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPTVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSVLLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKNILPLYVMNNEISGNGVTLSRLEGTDNIELYNSM
                     FAINYTNFEIKKQVTEAFKDKRSVILNEDTLKKVGLSIGDTITLSKDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRDLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(49602..51014)
                     /locus_tag="KRH39_000238"
     CDS             complement(49602..51014)
                     /locus_tag="KRH39_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8510169.1"
                     /translation="MHKPSYKTVFHSYIIFFIIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLTLANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGSK
                     FVGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     TDIYDSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRLLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDYTLLKRAFSNLIVNAFTYGDKNTKVCVHITHI
                     TLNERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGG
                     NISVESILGLGSSFYIIFPI"
     gene            51514..52374
                     /locus_tag="KRH39_000239"
     CDS             51514..52374
                     /locus_tag="KRH39_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG8510170.1"
                     /translation="MKKQKSPILLEMLGLFLGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(52615..53172)
                     /locus_tag="KRH39_000240"
     CDS             complement(52615..53172)
                     /locus_tag="KRH39_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8510171.1"
                     /translation="MSDSEYPQMKTLETRNCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
BASE COUNT        19856 a         7286 c         7540 g        18504 t
ORIGIN      
        1 cttatatttt gtatttagta gagtgttgct aagtgaatat atttaaaatg gaggaaactt
       61 tatgaaacaa aataaatatg atgatgatag attttttaat aaatacagta aaatggaacg
      121 ttctacaaat ggattagcag gtgctggaga atggcatgtt ttaaaaaaaa tgttacctga
      181 tttcaaagga aaaagagttt tagatttagg atgtggattt ggatggcatt gtcaatatgc
      241 agttgagaat ggagcaattt ctgctgttgg agttgatatt tcagaaaaaa tgttaaaaga
      301 agctagaaac aaaacgcaac ttgataatat aaattacata tgtatgccta ttgaagatat
      361 taattttcca aaagatagtt ttgatgtagt tattagttcc ttggcatttc attatattca
      421 atcatttgaa gatatatgta agaatataag taattgtctt tcaaataaag gtaattttgt
      481 attttctgtt gaacatccta tatttactgc atatggaagt caagagtggt actgtgataa
      541 agatggaaat aatctacact ggccagtaga tagatatttt gaagaaggag tacgtaggtc
      601 aaattttcta ggagaagagg ttataaaata tcataaaaca ttaacaactt atttaaatac
      661 gttaatgaaa acaggatttg aaatattaga aattattgaa cctcaaccag aagaaaaact
      721 gttagataca attcctggta tgaaagatga gctacgtaga cctatgatgt tacttgtatc
      781 tgcaaaaaaa agataagtaa atataaataa tcattattgt agaaaaaaag ttgtagaaaa
      841 taattacact aatggagatg attataccag tggtattaat tacaccagta gtaaggaggc
      901 aaaagcattc gttgcttttg ccatttttta agttgcaatt atacttctac gtagtatatt
      961 tcgtttccat tttcttctat aagctttttc atatcttcaa attttaaaaa tacagtagca
     1021 gtattatcat ttggatgtat acctatttta tccattttta ataaatcctt atcaaataca
     1081 acttttacaa cagcctcttt atcatttaat ataccaaaag gagtcacagc tcctttagta
     1141 agtttcaagt atttttgaag tctttcttct gatgcaaaac taagaggttt actatcaatt
     1201 tgttgacgaa tatcttttaa atctgctttt ttgtctttac ctaatacaac taaaaaatgt
     1261 tcttttcctt tagcatcacg aagaaatagg ttttttacaa caaattccat atgtaatgtt
     1321 tttaattctt ccatttcttc aattgtataa actggtggat gatttacagc ctcataaggt
     1381 atgcttaact catctaattt tttatatact tcctgttgtt gattgttcat gatttcctcc
     1441 cattagaaat ttattttata tataaataat attatcacag aaaactttaa aagaaaacgt
     1501 tttatataat atagacaata gattatattt ttataaagaa gcaagaatac tcaataaaac
     1561 ttaaagaaat taaacaattt ctaaaaatac cttgaaaata gaaccttatt tgccatttat
     1621 aaattatata aaaagggatt atataaagtc atatatgaaa aaactatatt atactatctt
     1681 ttctttttgg aataaactat actttaaata tagatattta atttaaataa attttacagt
     1741 attaatagta aattatctta gaaaggaatg tgctttatat gtctacagac aaagttgttg
     1801 gaatcataga tgaagaaacg gcagaacttg caggaattga atatactggt aaaatttatg
     1861 cttcttctgg tgttataaaa catataaaga aaaaacatag atgtcaactc agtaaggata
     1921 tttttaatga tataatagac actataaaaa tggtattaaa gtcacctgaa tatataggaa
     1981 gtcatcctaa aaaacctgga aaaagtgtgg agtttataaa aaaaattgat gattatattt
     2041 tagtagctac agaattagat acaaaaaaag gatatttata tgtatcttca atgtatgcta
     2101 taaaagaagc aaaattagaa agtagagtaa acagtggaag agtaaaacaa tatgatggtt
     2161 aacataatat tattaatttt ccctaaaata ttgaaaaata ttattatata agttataata
     2221 ttaatataca cgtagatatt tattagttag atttgaggtt ggaaaaggca cccaatacgc
     2281 tactatagag tagttaatag agatgcagga tacgccgccc tgccaaatct aattaaatta
     2341 aaaagagagg attaattcct ctcttattgt ttttttgaga tactaattta atctataata
     2401 gaaagattga aggtcgttta tacaaaaaaa ttttcagtta tatacattaa actaattaaa
     2461 taagatattt ataatctata tgtgatacat attatatatt aataaaagaa aaatatatag
     2521 aaaatcctta taaaaattta aattgtaatg aataggaagt aattaaaaag aatttttatt
     2581 gatttttaaa aatttagtta gaaactatat cattatggta aaaaatgtat tataatgttt
     2641 agaaatatta taataaggag cgattaaaca tgaatctatc gaagaaaata gaagataaaa
     2701 gaaaagctaa acagagagct gaaaaagtta agaaagctaa aattgctaca gctggtgtgg
     2761 tattaggagc tgttactggt gcagtgagtg gagtactttt ggctcctaaa tctggaaaag
     2821 aaacaagaga ggatataaaa gatgcttcac aacaaattgc tgaaaagata aatatgaaaa
     2881 ctgtagatgt aaaaggcaaa gtatctgaga agctagagga taaaaaagga aactttatag
     2941 aatcaaagaa aaaaataaaa aaatatatag ataacaaaaa gagtgttgaa aattctagtg
     3001 aggaagataa aataatagat atagttgaac cagttagtgt tactgaggaa taatatggac
     3061 aatacaataa cgattaacat aagtataata tatgtattat tagctatact tgcttgtatt
     3121 gctttagttt atttaattat tgtacttgtt aaactacata agattttaaa taatgttgga
     3181 actatgttaa atgaaaatac tcaaaatata aatacaactt taaataatct tcctaatgtt
     3241 gtagaaactt ttggagaagt aggagaaaat atgaaagatg taacagaagt agttactgaa
     3301 gttacagcag attttcttgt tactaaagac agtgtcaaat caaatatgga tgttatagtt
     3361 gatatattga ctataattaa aaacattttt ctagataaaa aataataagt taatataaaa
     3421 taagcctata agatataatt taaacgacct taaaatagca tttataaggt cgttttttat
     3481 tagcttgatt tgacactatg tgtttttaat aattggtaat attcctcttt atcatttgaa
     3541 catatatatc tatgcttgtt gtttcaatta catccataag aacatcttta cattcaagct
     3601 caaaatcttc cattccttct aaatattcat catcttcatc aaaaaagata taagaattat
     3661 atagccattc ttcaaatttt tcttcaagta tatcccaata cgaactccaa aataatccac
     3721 aacctgatat ataagatgct ctactttgat ttatataaac ctcctttaga agcttttgta
     3781 atctactgct ataaagaaaa tctgctcttc cactgcttga tattaattca tttaaatctt
     3841 ctattgacat taattcaact ttaccacttt tatcataagg agtataattt tcatcaaccc
     3901 atttatgtgg tttgttttct ctgcatactg ttgctatcaa ctctacaact tttgttctta
     3961 tcattttttt atcctaactt atttattttt tataaaataa tcatgatttt ataatttatc
     4021 tatttcttgt aatgctaatt taacagtttc atatctatca gcaccattta ttactacatc
     4081 accttttaca agtttattag cttctccacc tacaacataa acctttttac ctaaaccttg
     4141 cttataacta gacgcctcaa ttattaaaga atctttcaat tgccaatgta aaatctgagc
     4201 agccacttta tcagcatcac ctgtataaac gattacattt tcatacactt tattttcccc
     4261 tcccccttga gaatttgata tagatttgtt tagtatacct tctgctatca atttagcaac
     4321 tttatcttta ttattaacat aataatctgt atctgcttta ctatctacaa aacatacttc
     4381 tattaatatt gctggtgcaa tagtatgatt aagccaataa agacctctaa catcagactt
     4441 tgcgccccta tctttgaata ctgtagctaa cctagtattc actctttctg cataggtttt
     4501 gccattatta gtcttatata ttgtttctgt ccctactgga gttgaagttg tggcatttga
     4561 attaaaatga atctgaacag ccaaatcagt ttcctgttta tttgctatag cacattggtc
     4621 tgctaaatga ttacttgatt catcaactct tcctgtataa acagtagctc cacctatttt
     4681 aagccattta acaattaagt cattcagtat tcttgtttct ttgctttcgt ttatataccc
     4741 aacagctcct gtaccttttc caattaaagt gtgccctggt attattacta ctttcaaact
     4801 aacatctcct ttacaattaa atttaacttc tatttaatat atgcataacc tagaattttg
     4861 tttcttttta tatcttatac tcttattata acccattttg taaaaatata gaatattaag
     4921 taaataatgt taaaattata attttgtata taagacatta cttgattgca ataattttat
     4981 agcatattca tctattctat atccagttct ataatatata tgtttaattc cagcttgaat
     5041 tatagcctta gtacaattta agcatggaaa gtgagttaca taaatagagc aatttttaac
     5101 tgaaatacct tcttttgcac aataataaag ggcattttgt tcagcgtgaa tggttctaat
     5161 acaatgtcca tctctcattt catgaccaac ttcactacaa tgcttatctc cagaaatact
     5221 accattatat ccagtagaca ctattctgtt ttctgaattg acaattatag ctcctacata
     5281 agctctatca caagttcctc tttcagctac tgtttcgcac agtctcataa aatattcttg
     5341 ccatgagcat ctattttcaa gttttttatt caaatcatat acctccaaaa tttatatcta
     5401 atatataaga tttatacctt aaaaataata ttaaaaacaa atttatatga taaataaaat
     5461 ttattaatac tgtttttagt ctaaattatt tcattttaaa ttaaagttac aaggaatcta
     5521 aaattatata tatctttctt cgtaatatga atgttacaat ataaaaaact gtctcaataa
     5581 aatagagaca gttttacagt caaagtttat aatacaagtt catatttttc tttaaattct
     5641 tttacagctt cgggccatgc cttttgttct gtaatacctt tttctttagc tattttaatt
     5701 gcaatctctc ttatcattgc agcgccttct aatattccca tttttaatct cccttcaaat
     5761 atctttttgt atagaatata aatagtgcta agatttatta catgctgcat atacaatatc
     5821 tatacaacac ataacttgta aattcctttt attttcatac gtctttaacc gacatataat
     5881 tatattcaat aagttttaaa attatgaact caattataat tatttttatt tttttagaat
     5941 ctaaacacta aaaattgtag gtaaatttgt cttatgcctt tataaatatt tctacagttt
     6001 taattactgg acatatctat ttttattata aaaagcgatt aaaaatggag aatatcctca
     6061 taaaaactat atataagcaa aacggttaaa ggaaaaagaa gaaatttact tgaatgttaa
     6121 aatgcgttgc ttgaagcaat gggcagtttt gcctataata tcggtaacaa ctgaaagaaa
     6181 ggtggttagc cctaatgtta agcgaaaaca ttaaaacaat caggaagtca aaaggacttt
     6241 cacaacaaga acttgctatc aaactgaatg tagtgcgaca aacaatatct aaatgggaac
     6301 aaggactgtc ggttcccgat tctgatatgt tgatttccat atcagaggtg cttgaaacgc
     6361 ccgtaagcac tttacttgga gaaactattg ttgagtcgga agttgataac ttaaaagtga
     6421 tttctgaaaa attagagatt ataaacttgc aacttgcaca aagaaaaaac atgagaagaa
     6481 aaatgtttca ttggttattt attttattat gtgcagtcat attaatagtt tttgtggtct
     6541 tagggagact aaatagtcct tacttggatt ggaattatac cgaccctgaa accgctgttt
     6601 ttggagtagc ttttcatgct tttgagtggc tatttgtcag attagtaccg attgtcctta
     6661 taggagcaat tattagaatc tttttgacac gtaagaaaat atgaagtaac tctgcttttt
     6721 ataatacagc aagcgttcct taaaagcgtc attctacatg gcaatcgagg acgactatgt
     6781 aaggaagaac ctttttgatt tccaactaag cgaagttatt gaggacgaca gtaggcccaa
     6841 aaccgcatta agtaaagtac aggaagcaag actgctggac ttatactctc atgcgtaaga
     6901 tgcaggaaat gcgaggtctg gtagcatgat ttacaacgat ttgtacaacg actacgagta
     6961 gaaatatatg gagataagta ttatctacga aatcagaaaa gaaatgactt gataatttac
     7021 tcatgttata attattgatg tgaaattaat aaatcgaaat tccttttttg gatttaacaa
     7081 tactgagtaa ggaaaaagtg aatgactttt gcaaggaggt gttaatatca ggcaagtaaa
     7141 gtcttttgtt tttccagtta tatccttaat attcttagac caaattagca aagttcttat
     7201 aggattattc ttaatggact ttgaaattga tataattggg aaatttttaa gattcaatcc
     7261 tgttcaaaat acaaatctct cttatggggg aaactttatt ggtattctat ctaatttatg
     7321 ggtattggta ttgtttaaca ttttagttat attagttatt atatctggat atgcttttta
     7381 taaatcaaaa aatgaacaaa caagctattc agtaaaagta attatgtctt gtggacttgc
     7441 tggtacaata tgtagcttga tagataaatt attttgggga ggaagtttag attttttgca
     7501 gataccaagc ttttttattt tcgacttaaa agactgctac cttactgttg cagaaataat
     7561 atttgttgtc ataggaattt tgcataatag agaaatatca atgaaagaat acatatattt
     7621 ttgctatcgt cagtttaaaa gataaattta gaagataaat ttaagtttca aggtaaactt
     7681 atttaatcac aacaaatagt tggcttactt aagcttttgg gctaaactgt gagcactaat
     7741 taagtctctg ctatctaaca aacggagcac aaaaataaaa ataaaaatta aatataaagc
     7801 acagttaatt ttgattaaaa agttgactgt cttttctttt gtagaaattt aagcataggt
     7861 aataaagaaa tgactgcatt taagcattat gtatataatt ggaggcaata aaaagccagc
     7921 aaagcccata aaatagagct gtgctagcaa catgatgagt tataagaaga taatcaaaaa
     7981 cttacgcttt attttaattt aaaaaaatga ttactgtaaa gaaatcattt tttattataa
     8041 tcactgatac atcaaatatt ctaaataaaa tttttattta tctggaattg tatatttttt
     8101 ctataatatt aactacagcg tacattgccc aagcacagat agccaataca aatactccca
     8161 tcattactaa atctaattta aatacttgag aaccatatac tatcaagtag cctattccat
     8221 atcttgaaac taaaaattct ccaactatta cacctaccca tgccatacca atattaattt
     8281 tagttaaatt tattaaattt cctgtgtttg caggtaatat gagtttaaat aatatttgag
     8341 atttagtagc tccaaagctt tttaacatct taattttttc ttcatctata ttcataaaat
     8401 aattataagc tgaaagtata gtaacaacaa cagaaatcgt tacagctgtt acgacgattc
     8461 cttctattcc agcacctacc caaactataa ttataggagc taaagcagtc ttaggaagag
     8521 catttaaaac taccaaaaat gggtctaata tttttgataa tttttctgac caccaaagag
     8581 caattgctac caaaatacct aatacagtac ctataacaag accaagaaca gtttcatata
     8641 cagatattcc aatatgttta aataattgac cactaaatgc atattgaata aataaattgt
     8701 aaatatcact tggtttgcta aatagaaatg tatttataac atttaagtca gctaaaagtt
     8761 cccagattac aataaaacca attaaaatta atagctggta gaataataca tttctttttt
     8821 ctctttttac acctttaaca tagtttatat atccttcaga tcttttatta agcctcatgt
     8881 gtatctagct ccttccataa tatgtcgaaa tacttttgaa aatcaggaac tttacgtgtt
     8941 ttgagaggcg tttttgttcc atccatgtta agttcaatat cgtaaatatt ttttatggta
     9001 gctggtcttt tagatagtat tgcaacctta tctgatatgc ttattgcttc tgctatagct
     9061 aaaaaagtgc aactaattgc ttaaagaaac agaattatcc acttcaacac ccatataaac
     9121 aatattaaga tttcctaaac tgctatccca aacgatacta ttaataatag tagataataa
     9181 caatctctta tcatctaaac tggcattatc aacttcatca ttaaatctct tcaaattact
     9241 aacaataata tcaatattaa tactgtcact actaatattg atgttactag attccaaagt
     9301 attaatctta tcctttaaaa tactaatctc attatttagg ctctcaatct gggaaactat
     9361 ataatcagaa gcagtactac cagtattttt tgcaagctct ataactaaat ttttaataag
     9421 tctttcctta tctaaaattt gatttttaag taaattagtc tcagatttaa aaatattaaa
     9481 aacattatca gtcttaatgt tattactatt gaatgcattt ataatagact cttctttaca
     9541 attcttaatt ttatctataa ctaaactttc aactaatgga ccattaagat ttttacagtt
     9601 gcaactatca gacccaagtg tttttttagt accacaaata taataatact ttaaatcctt
     9661 gtcagttttt ttgcctttat aggtaattct catattagaa ccacatttac cacattttat
     9721 aagaccagat aaaagagcag tttttccagt tgatatcctt ggagttttat cacgattttt
     9781 atctaattga ttttgtactt caagccatat ttcaggttct atgactccat ggtgttttgc
     9841 aattgctgct attgagttag atgtattttt agcataggta aggattccac attttctatc
     9901 tggagtacct acaacatcca taccactatt tttaaggtaa tctaaaattt catcattagc
     9961 ttttacatat gctggatttc taagtattct agataaagaa ctagggtcaa atttaccacc
    10021 acgaggaccc cttattccat tattatagac atatttatat aacttagata aggacctaag
    10081 ttctaaatat ttattgtata ttagctttac aatctccatt tgttcatcat ttacttgaag
    10141 cttatacata cttctttctt ttagattttc atctaagaat gtgatttttt cactgttgaa
    10201 tccatgaggt gtattgcctc caagccatct accactacgg gctaattcat acatattgtc
    10261 ttttatacgt tctgctattg tttctctttc taattgagca aatacacttg atataaacat
    10321 catagctctt cccattggag ttgaagtatc aaattgttct ttgatagata taaatgatat
    10381 gtttagttca tttaattttt ctattaaagt agagaagtca gatacatttc tcgatatcct
    10441 atctaatcta taacaaacta ggtaactaaa tttcttatct ttggcatcct gcatcatttt
    10501 cttaaattgt ggtctatcta tattgctacc actaaatccc tcatcttcat atatcaaaaa
    10561 atcttttaca ccaatagact ttgcataatc aatacataat tgtatttgat tttctataga
    10621 atcacctttt ccagtaaatc ttgattttct tgaataaatt gcagctttca aaattaaaac
    10681 cccctataag ttaggatata tatttgaaat tatttatata caacctttta agtaaactta
    10741 ttagaatgaa tttattaatt aggtaaattt atataattct actttttata atatatgact
    10801 atttatttta tttctttact attcaagttc taaatcaata ataaattttg atatttaatt
    10861 gtatcaggac aaatctctaa tttcatagat tattaatata aaatcatagg ttagtgtgaa
    10921 aaagttgaaa tttaaatatt ttaaaagata atttttttga actaataggt ataacaaaaa
    10981 tttatagggg gattgcatat ggaaatcaat gttatagaaa ttttccctaa agataaagct
    11041 aaacttaata aaatagaaat ggataaagct agttggtttg taaatataat aagtaaaaaa
    11101 tatcctaaag aagctttaaa tgacgcattt agtactttag aaaaagaatt aaatataagt
    11161 aaagctaata cataagcttt gattattgat tttatatact attggtttta tacgctatga
    11221 cttgacatta tattaatcaa atagtatatt tttaaatata gcacaatgaa gtgttaaaag
    11281 agcaatggag ctccatagat aaagtctatg gagctttttt gcgtatttaa cttaaggagg
    11341 ataggacttt gaataacttt gagattaaaa ctagaataaa atttggagaa ggctcactaa
    11401 aagctttgca agaattaaaa aacaaaaatg tactaataat aacagaccct tttatggtga
    11461 aatcaaaaac aatagataaa ataacatcta atttaataaa ctcaacatat caagtttttg
    11521 aagaaatagt accagaccca ccaatagaag tagtagtagc aggaatagaa gcatttaaat
    11581 ctataaatcc agacgtaata atagcattag gtggtggttc tgcaatagat gcagcaaaat
    11641 ctataatgga cttttctaaa aaaatattaa aaattaacga agtagagttt atagcaatac
    11701 caacaacaag tggaacaggt tcagaagtaa catcatttgc tgtaataaca gatagtcaaa
    11761 aaaaatgtaa gtatccatta gtgagtgatg atttacttcc agatatggca atactagacc
    11821 cagagcttgt aaaaactgta ccaaatttta taacagcaga tacagggatg gatgtattga
    11881 cacatgctat agaagcttac gtatctacaa attctaatga cttttcagat gcactagctg
    11941 aaaaagctat aaagttagta tttaaatact taataaaagc atataaagat ggaaatgaca
    12001 tagaagctag agaaaaaatg cacaatgcat cttgtttagc aggtatggca tttaaccaag
    12061 catcacttgg cataaatcat tctattgcac atgtattagg tggaaaattc catataccac
    12121 atggaagaac aaactctata ttacttcctc atgtaataga atataattca tcaatagtag
    12181 gatatactaa cacaacttac tctgatacag ctaaaaaata tgcacaaata gcagaattag
    12241 taggacttgg gagtgctaat ataagaatat ctgttagaaa tttagtaaat gaaataaaga
    12301 aactacaaaa agaaatgaat atgccaacta aattgacaca atgcaaatta agtttagaag
    12361 atataactaa tgaagaaaga gaaatagcaa aacttgctat aaaagatact tgtacattaa
    12421 ctaatcctaa ggtaccaact gaagatgata taataaacat attaaagaac attaaataac
    12481 tacattttat tacatgtaaa tataatatta tacaaatagg gaaaatgttt ttaaaaatat
    12541 aaattatata ttgacaaata taaaacataa aactataatg aaagtatcac aaaggcgtga
    12601 tactttagta gaaagcaatg gagcttctta ggaatattaa tattattctt atggagctcc
    12661 ttttatttta atcaaatagc tttttaataa actatattca aagaaagcag ggaataaatt
    12721 tgactgaaga atctaagcaa agagttattc aagaatatgt tcctggaaaa caggttacat
    12781 tagcacatat catagctaac cctaatgaag acatatataa gaagttagga ttagtacttg
    12841 ataaaaaaga tgcaatagga atacttacaa taacaccaag tgaagcgtca ataatagcag
    12901 cagatgttgc tacaaaggct tcaaatgtat ctttaggttt tatagataga tttagtggtt
    12961 cagtagtaat atcaggtgat gtaagttctg tagaatcagc gttaaatgat gttttagaag
    13021 tcttaggaaa tatgttaaac ttctcatcta caaaaatcac aaggacataa taatacaatg
    13081 aagaatataa tatttatggg aaagacaggt agtggtaaaa ctaccttatg tcaaaaacta
    13141 gaccagttag aattaaagta taaaaaaact caatcagtgg aactttataa taatgcaata
    13201 gacactcctg gtgagtatat ggaaaataga aacctatata atgccttaat aacaacatca
    13261 gcagatgcta aaattatagc aatagtgtat gatgccacac aagaagaaaa ttatatagca
    13321 ccaggttttg ccagtatgtt ttgcaaagac gtaataggaa ttataactaa aataaataaa
    13381 gctactgaaa acgaagtcaa tatagcttat gaaagactat cactagcagg agcaagtcaa
    13441 atctttaaaa tagacacagt tgataatatt ggacttgaaa aattatttaa atatctagaa
    13501 aaatataaaa atatagatga agtttaaggg gaaatatttt tttattcaaa aatattatta
    13561 aagtatgata aaataaaaaa atgtataaac tattttaaga agatttaatc tttaagcgag
    13621 gtaggaacat ggggaaaaaa atactattgg ttgatgatga accactgtta aggatggata
    13681 caaaagatat attagaaagt catggctatg aaataatagg cgaagcaagt gatggatttg
    13741 aagctgttga aatgtgtaaa aaacacaaac ctgatttagt tataatggat atagagatgc
    13801 caatacttga tggtataaag gcaggcaaaa taatatgtaa agaaaatcta gctggtggag
    13861 tacttttatt aacttcattt gaagataaag aatacgtaga aaaggctaaa agtataggtg
    13921 cttatggata tttagtaaaa acagcaagtg aaaaatcact tatcccaacc atagaaatgt
    13981 gtttaaataa ggtaaatgaa tttgaaaaaa tgaaaaaaga tttagaaaaa gtaaattcta
    14041 aattgaatga aagaaaatta gtagaaagag caaaaggtat acttataaga gagtttaaga
    14101 tttctgagga tgaagcatac actagaataa gaaaattaag tatggataaa agaactacaa
    14161 tgatggaaat tgccaaaatg attataatag gatacgatga ctaatacgat aaaaaaaata
    14221 tcaactgcac aaacatactt aaataataac gaaattgaaa aaattataga agtatcaaaa
    14281 tccttagatt taatggcttc tttttatgaa gctgatgttt ttatagatgt actaagtaaa
    14341 tttgatgatg aagcaattgt tgtggcacat ggtagaccta aagaaaagtc aatgtactgt
    14401 gaaaatgtta taggtaaaaa ggctttaatt gaaaatgaac caggagttat aaagtgctta
    14461 aaaacaggag aaagagtaag agatattaaa gctttaacac aggaatataa actggtcaaa
    14521 caaaatattc agccaataac actagatgac aaagttttag cagtactaat agttgaaaaa
    14581 gatataagta gtgaagttaa gaatgagttt aatgctagtg aagacgaaga aaataaaaat
    14641 ttacatcagt ttataaaact tataggaaat aatgactttt tagttgataa tttaaattca
    14701 gccatattaa tatttgataa aaatggaaaa ttaaaaatta aaaatcgtat tgcaactgag
    14761 atatacaaaa atcttgggtt tggatatgat atacaagata tgcattacaa tgatttagat
    14821 atagaaacca acacctttga aaatatatta atggatgaaa attatgatga gacaaatgaa
    14881 gtttcaatag gtaatatgtt ttttaaaata aagaccatat acatcaaaga tgatgaattt
    14941 aaggtttgca aaatagtaca agatataagt gatttaaaga gaaaagaaga agaattagtt
    15001 ttgaaaacag tggctataag agaagctcat catagagtaa aaaataatct tcatacagta
    15061 atatctataa taaaaaaaca aagtagatta tctcaaaatg atgaagtaaa gcagtgttta
    15121 gataacataa taaatagggt atttgcaata ctttcgactc attatttact atctaaagaa
    15181 gttgataata atatatctat acgagaaggt atcaatttat tagtgtcaaa tattcaaggt
    15241 ggctatttat ttagtaagga tatagatata cgtataactg gtgaagattt taaaattagt
    15301 ggagaaaaag caacagcaat acttttggtt ataaatgaaa tactacaaaa ttgttatgac
    15361 catgcatttt cggatagaga acatggtact atacaaatat taataaataa agataacgac
    15421 tatagaaata ttgctattat agatgatgga attggatttg atagtaataa tataaataaa
    15481 aatagcttag ggacatatat aatagatagt tatataacac agatgttaaa aggaaatata
    15541 gagagaaaaa gtagtgagaa tggtacagaa gtattaatta ctatacctac acaaattaat
    15601 atttaaataa taactcatct acaaagtagt agttttaatt gagcaaagag gcttaaggtt
    15661 attaatagat taagttaatt gaatgtattt ataaaaatta taagtatata ttcaatttaa
    15721 caggtattta ttataacttt aagcctcttt gcttgtttaa gacatactta aattttagga
    15781 ggtgagaaaa tgagagaaga gttattaagt gttggtattg acattggaac aagtactact
    15841 cagttaattt tttctaaatt aatagtagaa aatactgaat caaatttttc ggtacctaga
    15901 ataagtatag tagacaaaga aattatttat aaaagcaaag tttatttcac ccctcttatt
    15961 tcaaataatg aaataaatgg tcaacaaata aaagatatag ttgaaaatga atataaaaaa
    16021 gcaaatatat ctaaagaaga tatacataca ggagcagtaa taataactgg tgaaacagct
    16081 agaaaagaaa atgcaaatga tgtacttcat actttgagtg gatttgctgg ggacttcgtt
    16141 gttgcaacag caggacctga tttagaaagt ataatatcag gaaaaggagc tggagctcat
    16201 atttattcaa aagaacattc aactacaatt gtaaatatag acataggtgg gggaactagt
    16261 aacatagcct catttaaaag gggagaagta accgatactg gatgtttgga tataggtgga
    16321 aggcttgtaa agatagataa aataagtaaa aaaataactt atatatcacc taaaatagat
    16381 aaaatcataa aagataataa tttaaatctt caagtaggca caatggctac tgtagaaaat
    16441 acaaagccaa tagtggatat aatggttgac ttattaatgg aaagcatagg attaaagaaa
    16501 cagacacctt tgtttgattt tataataacc aataaatcaa taagtataga tgaaactatt
    16561 aaaaatgttt cattctctgg tggagtagct gattatattt actatgatgg agaaataaaa
    16621 gattattttg aatatggtga cttgggaatt ttgcttgggc aagcaataaa aaattgtgaa
    16681 taccttaaaa aagtaaatgt agttaaatct atggaaacaa taagagccac agtggttgga
    16741 gcaggttctc atactactga agtaagtgga agtacaataa cttacactag cgaaaagttt
    16801 ccacttaaga acttgccagt tttaaaatta tcaattgatg aagaaacagg tacttcaaaa
    16861 caaatcagtg aagctataaa aaataaactt aattggttta aactagaaaa tgatttacag
    16921 aaagttgcta tagccatgat aggaaagaaa aatccaacct ttttagatat acaagagtat
    16981 gctaaagggt ttgtagatgg aatgcaacaa atcatagaaa atgatttacc ttttatagta
    17041 attgttgaaa atgatatggc aaaagtgtta ggtcaagcaa tatatgcatt attaaattat
    17101 gaaaaagaaa ttgtttgtat agacagtgta aaggttgaaa atggagacta tatcgacatt
    17161 ggaaaaccta tagttgaagg taaagtttta cctgtggtaa taaaaacatt agtttttaat
    17221 taatagaagg aggtaagtaa atgattttaa atacaaaatt atttggacgt acttatcagt
    17281 ttaaaggctt atatgatgta atggctaaag ctaatgaaga aaaatcagga gataaattag
    17341 caggtcttgc agcagaatca gctgaagaaa gaatagcagc aaaagttgtt ttatcacaca
    17401 taaaattgga ggatataaga aataatcctg cagtggcata tgaagaagat gaagtaacaa
    17461 gaataataca agatgctgta aatgaaaaag tttatgaaga aataaaaaat tggacagtct
    17521 ctgaacttag agaatggcta ttggattcaa atactacaag tcttgatata agaagaatat
    17581 caagaggatt aacttctgaa atgatagcag cagttgcaaa attaatgtca aatatggact
    17641 tagtttatgc agctaaaaag ataaaggtta ctgctcactg taatacaact ataggagaac
    17701 aaggaacatt atcagcaaga cttcaaccta atcatccaac agacgaccca gatggaatac
    17761 ttgcatcact tcttgaaggt ttaacttttg gtataggaga tgcagtactt ggattaaatc
    17821 cagttgatga ctctgttgag agtgttacta aagtattaaa aagatttgaa gaagtaaaac
    17881 aaaaatatca aataccaact caaacatgtg tattggcaca cgttactaca caaatggaag
    17941 caataagaaa tggtgctcct gcagatttaa tattccaatc tatagcaggt tctgaaaaag
    18001 gaaatgaagc atttggattt aatggcgaca ctatagaaga agctagacag ttagccttaa
    18061 aacacggtac agctacagga cctaacgtta tgtattttga aactggacaa ggttcagaat
    18121 tatcttcaga agcacatcat ggaatagacc aagttactat ggaagctaga tgctatggat
    18181 ttgctaaaaa gtatgaacca ttcttagtaa atacagttgt tgggtttata ggacctgagt
    18241 acttatatga tgctagagaa gttgtaagag caggacttga agaccacttt atgggtaaac
    18301 tacatggttt accaatgggt gttgatgttt gttacactaa ccatatgaag gctgaccaaa
    18361 atgatatgga aaacttagcc atactattaa caacagcagg atgtacgtat tttatgggaa
    18421 tacctcatgg agatgatgtt atgcttaact atcaaacaac aggataccat gaaacagcat
    18481 cacttagaga aatgtttgga ctaacagcaa taaaagaatt tgatgcttgg atggaaaaaa
    18541 tgggattctc taaagatggt aagttaacag agttggcggg agacgcatct gtattattag
    18601 gataggaggt atttaatatg aatgagaagg atttaaaagc attggtagaa caattagttg
    18661 gacaaatggt tggagaatta gatactaatg ttgtgtctga gactgttaaa aaggcaactg
    18721 aagttgtagt agacaacaat gcttgtatag atgatataac agaagttgat ataagaaagc
    18781 aattactagt aaaaaatcct aaggatgcag aagcttattt agatatgaaa gcaaaaacac
    18841 ctgcaagatt aggtatagga agagctggta ctagatataa aacagaaaca gttttaagat
    18901 ttagagcaga ccatgctgct gcacaagatg ctgtattctc ttatgtagat gaagaattta
    18961 taaaagaaaa taatatgttt gcagtggaaa ctttatgtaa agataaagat gaatacttaa
    19021 caagaccaga tttgggaaga aaattttctc cagaaactat aaataatata aaatctaaat
    19081 ttggtacaaa tcaaaaggtt ttaatattag ttggagatgg gcttagctca gctgcaatag
    19141 aggcaaattt aaaagactgt gtgccagcta taaaacaggg tctaaaaatg tatggtatag
    19201 attcaagtga aattttattt gttaaacatt gtagagttgg tgctatggac cacttaggtg
    19261 aagaattagg ttgtgaagtt atatgtatgt tggttggaga aagaccagga ttagttactg
    19321 ctgaatcaat gtcagcatat atagcttata agccatatat aggaatggca gaagcaaaaa
    19381 gaacagttat atcaaatatt cacaaaggtg gaacaactgc agttgaagca ggtgctcata
    19441 tagctgaact tataaaaact atattagata aaaaagcttc aggaatagac cttaaataaa
    19501 gtcgagggag tgtagaatat gaaaaatgat gtaattcgtc ctaatatact aggaattaaa
    19561 ataatttcaa atataagtcc agaaatggca caaaaactag aattaaaatc acatcacaaa
    19621 agcttaggtt taataactgc agactgtgat gatgttacgt atacagctct tgacgaagca
    19681 actaaagctt cagaggttga tgttgtatat gcaaaatcaa tgtatgcagg tgctgcaaat
    19741 gcttcaacaa aacttgctgg tgaagtatta ggaataatag caggtccaag cccagcagaa
    19801 gtaagaagtg gtctaaatgc agtagttgat tttctagagt atggagcaac ttttataagt
    19861 gcaaatgatg atgattcaat agcatactat gctcactgtg tatcaagaac aggtacatac
    19921 ctttcagagg tagcaggaat aagagaaggt gaagcattag cttatttagt agcaccacca
    19981 cttgaagcaa tgtatgcact agatgcagca atgaaagctg cagatgttaa gatgtgtgaa
    20041 ttatttgcac ctccaactga aacaaacttt ggtggagcat tacttacagg ttctcaatcg
    20101 gcttgtaaag cagcatgtga tgcatttgca gaagcagtaa aatcagttgc agacaatcca
    20161 acaggatttt aaggatagtt agatggatgc tataggctta atagaagtaa taggatatgt
    20221 tgctgcaata gaagcttctg atgcttgttt aaaatctgca aatgttaata ttgtaagagt
    20281 tgataaggtt ggggcaggta ttgttacttt aacaatatgt ggagatgtcg gtgctgtaaa
    20341 agctgcttta gaagcgggtg aaatagcagc ttccagagtt ggtacattaa gaacatcaca
    20401 tattataccg agaatccata atgaagtaac aaatgcttta ttcaaaacta aagaacctaa
    20461 agtctgtgaa gtttcagaag ataaagaaaa aaactttgag attacattgg aaatagctga
    20521 taatgtatcg aaaaatttat cagaaaaaga aaatgtaatt ttagatgtca atgaaaacag
    20581 tggagatact gaaaatttat atagtgatat agataaaaca aacaaaaaaa ttgaaacagt
    20641 tgatgatagt ttaacagaag ctaatgagga aaaaatagaa aacactaatg ataaagaagt
    20701 attggaacaa gttaaaatag aaaattccga ttttgataga gatgacctat caaagaacag
    20761 tgtaaaaaaa cttaaagcta tggctaagaa attagattct tcaataactt ataaagattt
    20821 aaatgttctt aaaaaagaag aattaataaa cttagttaaa aagcttagta gaagggatag
    20881 atagttatga cacttttaga taaagattta gtatctatac aagaagtaag aactcttgta
    20941 agaagatcta aagtggctca agagaaactt tctaaaatga gtcaagagca aatagataaa
    21001 atagtaaaag ctgttgtaga tgcagcattt aataatgcta ctaaattagc taaaatggca
    21061 aatgaagaaa ctggatttgg aaaatgggaa gataaagtaa ttaaaaatac ttttgcatct
    21121 atgggagttt atgaaacttt aaagaacaca aaaactattg gaataattgg tgaagataaa
    21181 gaagccaata cgatagatat agctacacca gttggagttg tggcaggtct tataccttca
    21241 acaaacccaa catcaacagt aatatacaaa actataatat ctctaaaagc aggaaatact
    21301 atagttttct ctccacatcc aaatgcaaaa aactgcatac ttgaaacagt taatataata
    21361 agtgatgctg ctataaaggc tggagctcca gaggattcaa tattatgtat aactaatcca
    21421 agtatagttg gaaccaatga attgatgaaa cataaagata caaacttaat actagcaact
    21481 ggaggagaag caatggttag agctgcttac tcttcaggaa caccagctat tggagttgga
    21541 ccaggaaatg gaccagcatt catagataaa tcagcaaatg ttaaattagc aatcaaaaga
    21601 ataatagatt ctaaaacttt tgataatggt gttatatgtg catcagaaca atctatagta
    21661 atagaaaaat ctatggaaga aactgtttta aaagagctta aaaatcaagg tgcatatgtt
    21721 ttaaatgaag aggaaagtga aagattagca aaattcatat taagagctaa tggtactatg
    21781 aatccacaaa tagttggtaa aagtgttgag gtaatagcaa atttagcaga cttaacaaat
    21841 gtacctaaaa atacaaaagt tctagttgca agagaaaata aagttggtca caatgtagcc
    21901 tattcaagag aaaaacttac accaatacta gcgttctatg ttgaagaaaa tgtagaagct
    21961 attatgaaca gatgcagaga aatattgtta aatgaaggtg caggtcatac attctcaatg
    22021 catgtaaatg atgaagaatt ggtaaaaaga tttgccctta atatgccagt atcaagaata
    22081 cttgtgaata ctccttcagc acttggagga ataggtgcat caacaaacct tgtaccagca
    22141 ctaacacttg gttgtggtgc aataggtgga agttcaactt caaacaatat aggaccactt
    22201 gatttaataa atgttaaaag agtagcttat ggaatcaaag aaatagaaga acttggaaga
    22261 gataaatctc aagttgaaaa atcaatgttt gactcaatgg ataaagaaga attaataaat
    22321 cttcttgttc aaaaactagt caaagaacta gcataactaa aataaacaat gaaacttatg
    22381 gctatcgcta taaatttcta aaaatataaa ctataaaaaa ctataaaatt aaatttttgg
    22441 aggataagat tatggcaagt gcaaacgcat taggaatgat agaaactaaa ggattagtag
    22501 gagcaataga agcagcagat gcaatggtta aagctgcaaa tgtacaatta gtaggaaaag
    22561 agcaagtagg tggaggatta gttactgtaa tggtaagagg agatgttgga gctgttaaag
    22621 ctgctacaga tgcaggagct gcagctgccg agagagttgg agaactaata tcagttcatg
    22681 ttataccaag accacacttt gaagtagatg ctatattacc aaaggtatca gctgagtaat
    22741 ataagaataa ttataaaatt taaattttga ttgaaaaaga ggctatctca aaaatgagtc
    22801 ttactaataa actcaatgta ttttgaggta gtctttttta tacatctaat aggagatgtg
    22861 ataaaatggg tgttttaaca gaaaacgaag taaaaagaaa aattggaaaa aacaaaaata
    22921 atataactga attttatata gaaaaaggtc aaataataac tccttctgca aaatcttatt
    22981 tagctgagag aagtattgag cttaaatatg ttgataaact ttcagatgta aacaatgaac
    23041 ctaaagaagt tgtaaaagaa gtagttaaag agaaaaaata taaatatact actgtatttg
    23101 gcgctaaatt ggatgaaaaa ccagaacata tgactcaatt aaatggaaat cttttagttt
    23161 ttaaagacca taaaagaata atattgagag gaaaaataga ttctcttgaa tcagaaatac
    23221 taaaggtaca gatactttgt cagaaactag atatgccaaa gcttgttaag gaattgcaag
    23281 aagtattaga ctttgtaaga aatataatga gatgtgaagt tttagatgaa aagctagggg
    23341 actttaaact tttaggatta actcctcaag aacttagaga acaatctcac tatcctaaaa
    23401 aatactttgg tataggacat gagttcccaa gttatgaaat gggagaagta gtagtagcta
    23461 taaatagtat aagaagtaat acacgagagg ttgaacttgc agcctatgaa gcatttaagg
    23521 gtgagtatgg aaatgttcac agagaagatt taataaaggc acttaataga ttatcgtcag
    23581 tattttggat aatgatatat aaggttagaa ctaataagta taagtagttg gaggacataa
    23641 tggagaatct attaaatgaa atagtggaag aagtaataac tagagttaaa aaagaagcat
    23701 ttatagaagt tgaagcatca ggaagacacg ttcacttaag tagagaagat gtcgataaat
    23761 tatttggaga aggatatact cttacaaaac taaaagacct atctcaacca ggtcaatatg
    23821 catgtaagga aagagttaca ataactggtc ctaaaggaag tataaaaaat gtagttgtac
    23881 ttggaccatg tagaaatgaa actcaggtag aaatatcttt aacagatggt tcaactcttg
    23941 gactaaaagc tcctataaaa caaagtggag acttagaagg tactttaagt attaaaatat
    24001 caacacaata tggtgaagtt gaacttgata aggggttaat ggttgctaaa agacatatac
    24061 acatgactcc taaagatgct gaaaaattca atgtatgtga taaagagata gttgaagcta
    24121 aggtgatggg tcaaagacct ttaacatttg atgatgtagt aataagagtg agtgacagtt
    24181 ttaaaactta tatgcatata gactatgatg aagccaatgc atgtggttat tcaaaaggaa
    24241 ctgttgctaa aataataaag aaagcataaa tttaacctac tatataaatt acctctaggg
    24301 aggatttaat atgaattatg ataaccttgt agatttaata acggaagaaa tctataaaaa
    24361 aataaatagt aatgaaataa aaatatctaa taaacctaaa gctgtgattg tatttgaaca
    24421 agataacaat aaatttaact tattaaaaga tgaatttgaa atagtagaat ttgataaaag
    24481 tataagggaa tgtgaaatag tgatagtttc aagactttgt atgagggggc tatcgaatat
    24541 agcacttgga aattcaacaa gtgatgaaga aagatttata ctaaaaatga ttatgaaggg
    24601 caaaaaagta tatgtcctag atgaaggaat tgaatacaaa aaatataagg atactgcacc
    24661 aaaagcatta tataagaaat ttatgtcatt tgaagatgaa atatgtaaat ttggtgtaga
    24721 aattataaaa gacttaaatg ttataactaa aaataagtta aatataaaga aagaactaga
    24781 aagtactaga ggttctaaaa aagaaggtac aaaagtttta gataaagatg ttttaaattt
    24841 aaccagtgta gaaagtgtaa aaatagataa tgaatttagt ttggatttaa ggaataaaaa
    24901 acttatatcg gaagctgact taagaaaacc aactataaat ggagttaaaa acatattagt
    24961 taataaaaaa agtataataa caccattagc tgtagatttt atgagaatac accatctcaa
    25021 actaaagaaa gtgtaaggag gtaattttat ggatatagga aaagttgtag gtaatgtttg
    25081 ggctacaaga aaagatgaaa aattatgtgg ccaaaaactt ttagttgtaa aaattttaaa
    25141 aactaaagac acatacaagg aaggcttcat ggttgcagcc gataatgcag gagcaggaaa
    25201 tggagactta gttcttatat ccaaaggttc ctcagctaga gagtcgattg aaaaatctca
    25261 tgttccagta gatgcaacta tagttggagt agtcgattcg tttgaggtgt ttgatgaata
    25321 aaagtatagg agccatagag tttaaaagca ttgctaaagg tataaaagta tccaatgaaa
    25381 tgataaagaa atcttcagtt gatgtattgt acttaaaaag tatttgccca ggtaaatttc
    25441 taataatagt aggtggagaa acttcttata taaatgaatg tgtagattat ggaataaaac
    25501 ttggagaagg atatattgta gataacttcg taataaatgc aatttcacaa gaaatattag
    25561 atggatttaa aaataagtat caaaagctag atagtatagt ctctataggc gttgttgaaa
    25621 gtagtaaagt atgtgctgga ataaaaatgc ttgataagac tttaaaatct ggcgacttag
    25681 tattagtaaa acttcaatta tcttttgcta taggtggcaa attagtatat atagtagcag
    25741 gagatttaag tagtttagaa tatgcattaa aagaaagtga aaatgtagta agagaaaaag
    25801 aagtaatata taagactgta ataccatcag tagacagtca aataataaaa agtctaataa
    25861 aataatgggg ggaattatga gtataaacga aattataatc tatgtaatgg tagtcttcat
    25921 ggtacttgga gctatagata aatgtatagg taacaaattt ggtcttggag aacaatttga
    25981 agaaggtata atggcaatgg ggtcacttgc acttgcaatg gttggtgtta tatgtttagc
    26041 accagttctt gctgatgtat taagaccaat tgtagtacca gtatttaata tacttggagc
    26101 tgacccagct atgtttgctg gaagtttatt agctaatgat atgggtggtg cgccacttgc
    26161 acttgaactt gcgaaagacc ctaatgctgg gttatttggt ggattaatag taggggctat
    26221 gatgggacct acaatagtat ttataatacc agttgcgctt ggaataattg aaaaagaaga
    26281 ccaaaaattc ttagcaacag gaatacttgc aggtataata acaataccta ttggagcatt
    26341 cgttggtgga ttagttgcag gattcgaggt aatgatggtt cttagaaact tagtgccaat
    26401 aataatattt gcagtagtta tagcacttgg attattaaaa ttcgaaaatg ctatgataaa
    26461 aggattcaca tactttggaa aaggtgtagt aatagttata actttaggac ttgctgctgc
    26521 catagttgaa gctttaacag gaatagttgt aatacctgga atgaaaccaa tcagtgaagg
    26581 tatagaaata gttggagata tagcaatagt tttagcagga gcattcccat tagtgtttgt
    26641 tataacaaag gtatttaata aaccattaat ggctttagga aaagttcttg gaatgaatga
    26701 tgtatcagca gcaggccttg ttgcaagttt agctaactgt ataccaatgt ttggtatgat
    26761 gaaagatatg gacaatagag gaaaaataat aaatgtagcg ttctctgtat cagcagcatt
    26821 cgtatttgga gaccacttag gatttacggc aggatttaat tctaatatga taacaccaat
    26881 gatagttgct aaattagttg gtggtataac agcagtattg ttagctatgg ttatcgcaaa
    26941 taagacacta aagaaggaga atgtataaga tggatatatc aaatatagat aaaaatttaa
    27001 ttgaaacatt agtaagacaa ataatagaag aaaaaataag tggaactaaa gacacagtag
    27061 attttgttag aaataaagac attagtggaa taacttctat aaagttacca actgtaaaag
    27121 ttagtgaatc tgatagatta gatacaggga atccaagtga tgtagtttac acaaaagatt
    27181 tatttacatt agaggaaagc ccaagattag gttgtggtat gatggaaatg aaagaaacaa
    27241 cttttgattg gactctaaac tatgatgaaa tagattatgt aatagatgga actttagata
    27301 taataataga tggaagaaag gtttctgctt cttcaggtga attaatattc ataccaaaag
    27361 gaagtaagat acaattttct gtacctgatt atgctagatt tatatatgta acatatccgg
    27421 cagattgggc atctcaaaat taataaaaat caagttaagt taataataat caaataaata
    27481 atttaaaata caaactctta aataagcaca catattaaaa aaagctatga attaaaattc
    27541 atagcttttt ttataaagaa tgttatattt tagacgtatt tttactggga gacatattat
    27601 attttttatt aaataatcta ataaaatgct ctacatttgg ataaccaaca ttgttgctaa
    27661 tatcagcaat agaaatattt gtattaatta acaaactttc agctctttca agtctaattc
    27721 tttgtaataa ttgagtaaaa ttacaaccag caatttcttt aataatttta gaacaataag
    27781 gtatagaaaa gtgaaattca ttagcaagtt cttctaaagt tatatcaaaa taattatctt
    27841 ggatataagt aagaatatgt gtgtttttta gattatcttt ctttgtcata ttaggaactt
    27901 ctatactatt ctcatagcct ctaagtaact ttgcaaaaaa caccattatt aaatgattaa
    27961 gtatattatt gtaatatcta tcttgtttac tatattctga aaacatctct aaaatagaca
    28021 tttctattga tttatcatta tttgtatgaa aaagaatata gtcattctgc ttgtttttat
    28081 aaatactata taagtttcat cttttatgtt gcacttttat acaccttgct tcaccaatat
    28141 catgtgtaac caaaatagta gacttattct cattcttaat aatactataa acatcatcac
    28201 aaactaataa tctagtctga taatcaagag ctgaaaaagg ttcatctaaa agtagaatat
    28261 ctggatttac agaaagtgtt ctaataagcg caactctttg tctcatacct ccagataatt
    28321 cttttggata catacttcta aaatcccata atccataagt ctttaaaagg ctttcaactc
    28381 tatctaaaga ttttttatct tttttgcctt gaatttccaa tccaatagtt atattatcca
    28441 ttatatttct ccattcaagc aaattatctt tttgaaacat atatccaata tttccagtaa
    28501 tctttatatc accactagtc ggttttaaaa gattagtaag aatatttaat attgtagatt
    28561 taccacttcc agaaggacca agcaaagtta atatttctcc ttctttcaag ttaaaattga
    28621 catcctttaa tacttcaagc tcgccttcct tagtgtagaa gttcatattt atatttttaa
    28681 cttctactat gttgtcattc attatgccac cccctataac ttttagctcg tatatattat
    28741 gttattaaca atatgaccta tttgtaccat ttttcataaa tataatataa atattttgtt
    28801 tatttacgta ttttcttagt aacttgtacg tataaattat agataaataa attataaggt
    28861 aggggataaa tttgagtaac ataataacga atttaataga gtcaatattt actaatccaa
    28921 taactatatt gtttttactt gcaataactg tgtattctgt ttttaaaata atagaaaata
    28981 acagagtata ttcatttgta agttcaaaat tagacgaaat caatagagac tataagacaa
    29041 gtgagtttta taaaaaggta agggatgact actatacata tagtaaagaa aatccttatg
    29101 cagacgtaaa tataacttca tttatagagg aagttgtatc tgatttaaag cataataatt
    29161 tgccattgtt agaaaaaatt agaagtataa aaaattcatc ttcaataagt atattacttg
    29221 gggttttagg tacgtttgtt ggtctttcta caatgctttt atgtgttgat actaaagaca
    29281 ttataaattc acttccttca actattagta gtatgcaaac tgcatttaca actagtatat
    29341 ttggagttgt attttctcta attataggtt actttactaa gataaaagat tgtgaacatg
    29401 ttttgataca aataatgtta aaatctgaaa atttactaac atctgaaata actcatttta
    29461 agtcagaaag aatggattta aaagtagaag aggtaaaaaa tacaataaaa caaataagta
    29521 agtctataga agctattgaa aaatttgata aaatatctaa agatttaaat gactttaatg
    29581 atgaatttat cagtggtata gaagctttaa aaagcctttt agaaggttct caaagttcta
    29641 ttaagacatt tgaccagagc gtaagaaaat tagataaaca gtttaatatt ttaaatataa
    29701 aatttgtaaa gctttttgat aaatatgata atcaagataa tataaataaa gagattttat
    29761 ttgatataaa agaatcttca aaaaatatat acaatgccac tgaaagccaa ttcaaaatta
    29821 gagattatat aaaaaatata aatgcaggat ttgctctata tgaaagaagt gctcaagatt
    29881 tattaactaa attaatgacc catgaaaata aaatatctca aaatcaaaaa atccttttag
    29941 atgaaaagtt taccttagat gactcaataa aaaatctctc aagtattatt gaaaactttt
    30001 ctaatgacct acaagttaaa ctggatatga tgtttgaaaa ttctttagat attcaagata
    30061 agttagatgt tatgtttaat aactctttta tgaatgatga agttcctctt gatagtgaag
    30121 aattatttaa ggatgatgtt aataatgtat ttaatccttt tagtgaagaa atttatgaga
    30181 tagaggataa agaaataaag gtcataggtg aggatgagtt aaatgaataa taaatataga
    30241 agaactgtta acagagaatt tgaaaagagt agcttttggc caacatttac agatttatta
    30301 tctactgtat tgatggttgt aattcttatc ttatttagct cagaaagtat ttcaggttct
    30361 gttgaacaag atttagctaa gaatgtcaat gcttctgttg aagaaacttt taaaaaaagt
    30421 ggaatacctg tgaaggtaga taaatctaat gggcaagtaa cttttggtga aaaaaccatg
    30481 tttgatgtag atagtgatgt tttaaaacca gaggctaaag aaatgctaaa aatgtttgtt
    30541 ccaaagtaca tagagactat ttataaagat tatggtgact atatatccaa aatagttata
    30601 gaaggccata cagatgatgt gggaagttat atatacaact tagatttatc gcaaagaaga
    30661 gcatatagcg ttgctaaatt tatagttgga gatgaaatag gtgactataa atacaaagat
    30721 aaagtgacta agcatattat tgcaatagga aggtcaaaag ctgaactcat caaaaatggt
    30781 gacaatagcg tgaatagaga tgcatctaga agagtagaac taaaatacga aataaatatt
    30841 aatcaaaata aataattaaa tttttataaa aaaaaagaag gcagttggtg tgagctttaa
    30901 gccttcttta aggaaaaagt ttatttattt ttgttgatac ttaacatatt atagttttta
    30961 gaagcagttt gcaatatatt aaacgtaaat ggtcgtttaa aatgcataac tggatactac
    31021 tgatgtaaaa tatttcattt tatataatat aaaaattata cagtgtcaat tttctatatt
    31081 attgtactat aatctattat ttaaaatatt atgtaaaaaa atatcataaa aacgcatatc
    31141 atgttataat tactagagta ttatgaatat ggaggcgtag tttgtgatag aaataagtat
    31201 aaaaaataaa aaaaattcta aaattaaaac tttgatactt ttattgatag tatctatatc
    31261 gatttattta actggttgta ctacatcaga taatggaaat aatcaatcaa ataataatga
    31321 ttctttgtct agtagtatat caagtgataa gaatgtaaaa attcatttta ttgacactgg
    31381 aaatagtgat gcaatattga ttcaagatgg taaaactttt acattgatag atgggggaga
    31441 taatgatgat gagggattaa tggtagatta cctaaacaat caaggtgtaa aagatattaa
    31501 atatcttata gctacccatt ctcatgcaga ccatttaggt ggacttgatt ctgttgtcaa
    31561 aaactttaat atagaaaatg tttttgtatc gaatggttca gcagaaacaa agagttatag
    31621 ggactttata aatgctttag caaataaggg tttatcccca agtgtaccgc ttgaaaataa
    31681 taagttctat ttagaagatt cttattttga agttctaaat acaaatgggg gagatactac
    31741 taatgaacaa tctctagtcc ttgtatatac taatgggaat gataaagttt tatttacagg
    31801 tgatgctgaa gaaggtaccg aaaaagaaat actacctaag cttgagaaag tagacctttt
    31861 aaaagtagct catcatggtt ctcgtagtag ttcatctcaa gagtttttag ataaagtaaa
    31921 tcctgaatat gcggttcttt tagtaggcaa gggtaattca tatggtcatc cacatcagga
    31981 gacaatgaat aaattaaaaa aaatgggagt aaaagtgcat agaagtgatg agtgttcaga
    32041 tataatattt gaatcaacag gtgatggagt atttacaagt tgtaaagatg gaagttataa
    32101 taaaggcgtt agagaagatg gaaactctgg aaaatcaaac agtaatacaa gtaaaaatga
    32161 ttcttttaaa aataaacaaa atacatccaa ttcagaagaa atagtgtatt ttactccaaa
    32221 aggtaagagt tatcactcta caaaaaattg tagtgggctt tcaagaagta aaaaaatatt
    32281 atcaggcaca attgcagaaa gtaagaaaaa tgacccatgt gatatatgtt atggaaaata
    32341 acaaaaaaac aaccaattta tctataaagc ttctgaactt taaatcattt aaaaaataaa
    32401 ataaatgcat aaaaaaataa gctataatca atagataaca cattttatct atacgaatag
    32461 cttatttttt taagtatttt ttaatagcta tttaataact ctaaatacac cagttacaat
    32521 acctacaatt tcaacttctg agtcattgag cataattggt tccataaaat cattttctgg
    32581 ctgaagtctt attaaatttc cttctttaaa aaacctctta actgtagcag attcaccatt
    32641 tataagtgca actactatct gagaatttaa ggctgtattt tttttgtcta ctacaacgta
    32701 atcttcatct aaaattcctg cattaatcat actttcgcct tttactctta atacaaaatt
    32761 gttactgccc tttactaaac tggcagggaa tggaatgtat tcctctatgt tttgttgagc
    32821 taaaatgggt tctccagctg ttatttgacc tactaaagga agctctatta tttcttgatt
    32881 agctccagaa acatcattta ctttactacg ttctaaaact tcaatagctc ttggtttagt
    32941 agggtccttt cttatgtatc ccagtttttc aagtttattt agatgagagt gcacagttga
    33001 agtagatctt aaccctacag cagtacatat ttctcttaca gcaggtggat aaccttttaa
    33061 tatgatttga gactttataa attccaatat caagacttgc ttttcagtta gatctaaata
    33121 cataacatca ctcatttcca aacatatttt gtttttattt ccatacactt tatattttta
    33181 attataacat aatccattta tatctgcaaa catttgttcc tgatttttaa tcgtcctcaa
    33241 tccattcaaa attcttgact atatcacgtt ttacattttt cttatgagct gcataaagct
    33301 gagtagttgt gacattatca tggtcaagta agttttgaac atcatatata gaaaatcctg
    33361 tttggattag agaagttgca gccgttgccc ttaatttatg gggactatat ccattgtctc
    33421 tagtagtatc aagtggaata gatgtatatt tttttaccag tgttcttata gcctttgctg
    33481 taattctctt attttgaaga gacaaaaata aagcatcttt ttcttcatca tttaatagtt
    33541 catctcttgt tctttcgttt tgcaaataat cttttattac atgctcacaa gtatgattta
    33601 taggcattaa aacttcttta ccacgttttc tatatatttt aaattcacct cttgaaaaat
    33661 taaaagagga gatattgagt tctctaagct catttaatct aagcccgtat gtaacaaaca
    33721 atgctagtat agctttatca cgtaacttag tctttctcca atataccttt tctttctctg
    33781 taagcccttc acctgtttct actgattcaa gcatcttagc aacttcatct atttcaagac
    33841 gcttaatagc atctggctga ggtttaggaa gttttattgg attgaatcca tcagttatat
    33901 tattatctat ctgagagttt ctatataaga atttaaatag agtagagatg gatgattttt
    33961 ttcttgctaa agctctattg ttattttcaa aaatcaatgt atttttttct gtatttttat
    34021 aatatctact acaataatca cctaagaata aatttatatc tcttgatttt attgtattaa
    34081 aatcatcttc tgtaatatct tttacacaat ctgcatttgt taattctttg gtttcaatca
    34141 aatatgaaca gaaaaaatta atatcttcta aataagcaag tcgtgtagaa actgcaacag
    34201 aaccttttag atatataaaa taatccttca taaaagcagg aagtttattt tccaaaagta
    34261 gacatttttc tatgagttca ttttctttga tgacaatatt atcaggttta tctgatttat
    34321 tatgtacttt tataattttc ttatcattat tcaaaattaa acctcctaaa actaaaatat
    34381 aaatctttta tattattttt tgatatttat accaagtgag tataaatatt taatttaatc
    34441 attttttgta tttttaagag attagcagtg ctttagatga aatcgccact aactattccc
    34501 taaatccaca tttaaagact agttaaattt aagtactctt tttttatttc tcaatatatt
    34561 tatagaaata ttttaaaata tattgagaaa ttttttatgc tctactaaaa tcttttttat
    34621 ataatatttt tatatgtttc taacgaaacc aaatattagt acaatatgta tttatttaac
    34681 taaagttttt aaatgggctg actaagctta aaatatagga tattatgaga gttactattg
    34741 aaaaaataaa ccctattgtc ataaagaaac ttataaatat attatactta actattaaac
    34801 tggatatatt caagttatca aatccaaata aatatgtaat tgaacttatt acaaaaataa
    34861 ctataactat aaagctcaga ccatttaaac tattttttaa atcagcacta ctaagtgaca
    34921 tatgtgtaga tatagaatac attaaaaaca taaatataaa atacttcaat gaatatacac
    34981 taggtgttaa caacattatt cttataaaat tggaaatatc acctaaaata atgtgtataa
    35041 acccatttat atttaaatta ttaatcatac ttacatataa ctttaaatct atattttgag
    35101 tcactttcat ataactatct ggcagtaata tcttaaaaat taaaataatc attattgttc
    35161 cacctataat aggtgcaatt cctataaaga aatttcctat actttgataa agattatctt
    35221 ttttatagct atgacttaca tatcctagta taccatcatt tttactttct attggtctga
    35281 aaaacttaac tgaatttatt ttatgcatga aaattaaaca cattatcaaa tgactcaatt
    35341 catgaacaaa tgttcctata gctccagtta caataattcc agtttttcca aaagatgaaa
    35401 agataaattt attatttaga ttttctgtaa tgctaaaaat caatccaaat acaataaata
    35461 ttcctatgaa ataaaaaata tttattatac ttatttttaa aatttcgact atataaatca
    35521 taagttaaag cctcatgtaa aagtatttaa ctactctact tcatatccat tatagtttaa
    35581 aattttacaa gcattttcta tatctttttc ttttactaag atatagtctg tatcatatgt
    35641 agaaattgca aatatgctta ttttgttgtc tgctaacaag ctacttaact tagataaaat
    35701 tcctacaagt gaaaagtcta aaggacctaa tatcttaagt attctccact ccttttctga
    35761 cttaacatca gaaggaatat tattatttaa acatacaaca cttaattcat catcagttct
    35821 agttatagag taaaacttct ctgtatctat ccatgttggt attttactat cattatttaa
    35881 tctacatact gcatactctt ctaccaataa ttttaatttc ataatgtata tttcctccaa
    35941 tcttatttgt ttacttaaga ttatacatta aaagttacat aaaataaaac atataaagta
    36001 aaaaaggttc actatagtat cctatttcta tagccaacct ttaaaactta attaaattct
    36061 aataaagaga tatattatct aggttcaatt ataagtttaa tggctgtttt cttatcacct
    36121 tcgatttcta tatcagtaaa tgcgggaatg caaactaaat ccattccact aggtgcaaca
    36181 aaacctcttg ctattgcaat tgattttatc gcttgattta gagctccagc tcctatagct
    36241 tgtatttctg caacgcctcg ttctcttagt actccagcca aggctccagc tacagaatta
    36301 ggattagatt ttgatgaaac ttttagtacc tccatttcaa catctccttg ttaatttgtt
    36361 aaagtatcca ttacctaaag tactaaatca taattgccat ttactaatta caatcatcat
    36421 ttaaagtata cttttataaa aatatacttc tataatagtt ttaagaaaac ctttattttt
    36481 tttaaccatt ttattatgaa aaaactacaa atttctttat attctataag cattttccaa
    36541 tatttctaaa tttattatat ctttgagcta taatttcttc tttatccata tctttataat
    36601 gatttaatct ttctaaaata taagctttta tttcttttga aacagcttct acatcttttt
    36661 gtgctcctcc aagaggttct tttataatct tatctattat ttttaaatca tataaatcat
    36721 tgcttgtaag cttcataaca tcacaagcct ctttagcttt agatgaatct ttaaatagaa
    36781 tactagaaag cccttctgga gaaatcacag aataaactga atgttctaac atacaaacat
    36841 cattgcccac accaatacct aaagctcctc cactaccacc ttcgccaatc acaaaagtaa
    36901 ttactggaac agaaagttta ctcatttcaa gtaagtttct tgcaatagct tctccttgtc
    36961 ctctttcctc tgcttctaga ccgcaaaaag ctcctgatgt atcaataaat gttattatag
    37021 gtctattaaa tttttcagct tgtttcataa gtcttaatgc ttttctgtat ccctctggat
    37081 gaggcattcc aaaatttctt tttatatttt cttttgtatc tcttcctttt tggtgtccaa
    37141 ctatagtaac attaaacttt cctatctgac caatccctcc aactatagaa gggtcatctt
    37201 taaaaagcct atctccatga agttctaaaa aattagaaca aatatgctct atgtattcta
    37261 atgtcgtagg tctctttata tctctactta gtgttacctt ttcatatgct gatagatgtg
    37321 aaaaagcatc ttccttaagc ttttctaatt tctcattaag tgaatttatt ttatcactta
    37381 aatctatatt atttgcctta gaaatagtta ttaattgttt aatatcattt tctaagactt
    37441 ttattttttc actattattt tcaatcaaaa cagttcctcc tttatgccct atttcttgtg
    37501 catagcaaga atttgatata atacttcctt catttttctt ctatctacta tcatgtcaat
    37561 aaaacctttt tcaagtaaaa actcagaagt ttgaaatcct tcaggaagtt tttgatttat
    37621 tgtttgctct attactcttg gacctgcaaa acctatcaac gtatttggct cagagattat
    37681 aatatcacca agcatagcaa aactagcagt tactcctcca gtagtagggt cagttagaac
    37741 tgatatatat ggcaatgatt tttcttcaag tttagaaagt gcttgagaag tctttgccat
    37801 ctgcataaga gataccatac cttcttgcat tctagctcct cctgaagcag aacaaataat
    37861 tataggtaaa ttattttcag cagcatactc tattgaataa gttatttttt ccccaacaat
    37921 agaacccata cttcccatca taaaatcagg attcattaca caaataactg ttttaattcc
    37981 atttattctt ccataacctg tgacaacagc ttctgactct tttgtttttt ccttataaga
    38041 gtctactttt tctttgtatt taggaaaatt tagtggattt tgaaattcta catcagaatt
    38101 aaactccata aaagttcctt tatcaatcaa taaatttatt ctttttcttg ctcttaagcc
    38161 ataatgtctt gaacattttg gacatacaaa tgaattttct tctatgtcct ttctaaatac
    38221 atgactatca caacctttgc aataggtcca aaatttatca tctacactat tctttttaaa
    38281 gttgtcatct aaagaaatcg taacatactt agattctttt gatgataaaa atttttttat
    38341 cataatctat ttactcctta taatgtagca aattctttct tcataaagac atcttctata
    38401 aatttggtat gatgagtatc ttctctaaac tcctcacttg ttacaagtgc cttttgaaat
    38461 tctacatttg tattaatacc ttctacaata atttcgtcta gagctctata tattctactt
    38521 atacattctt ctctagtttc agcccaacat attaacttac ctatcataga atcatataaa
    38581 ggtggtatag tatatcctgc atatacaaaa gtatcaaacc taacaccaaa acctccaggt
    38641 aaatttaatg attctatctt acctggtgat ggagcaaaat tctttgatga atcttctgca
    38701 tttattctac attcaatagc atgacctcta aaagtaatat cttcttgttt aaaaggaatt
    38761 ttatccccat tagctataga tatttgaagt tttataaggt caagatttgt taccatctct
    38821 gtaactggat gttcaacttg tattcttgta ttcatctcta taaaataaaa gtttttatct
    38881 ttgtcaacta taaactcaat agtacctgca ccgatataac ctacaccttt tactatatct
    38941 actgcaattt tatgaagttt ttgtctttct tcgtctgaaa ggtatggact taaagattct
    39001 tctattactt tttgatttcg cctttgcata gaacaatctc tatcacctaa atgtattgca
    39061 tttgaatgtt tatctccaaa tacttgtact tctatatgtc taggattttc tataaacttt
    39121 tccatatata aatcactatt agaaaatgct gctagagctt ctgaacttgc catatcaaaa
    39181 tttgaaaaaa gttcttcttc ttttctaacg attctcatac cttttccacc gcctccactt
    39241 gaggctttta tcataacagg atatccaatt tctcttgcaa cttctaaagc ttcttcctta
    39301 gttttcaaaa cagattctga accaggaaca acagggatat tcaattccat catagtttct
    39361 cttgctctag atttatctcc catcaaaatc atttgttctc ttgttggacc tataaaaata
    39421 atattgttct cttcacattg ctttgcaaat tcaggattct ctgataaaaa tccaaagcct
    39481 ggatgaatag catcacactt taatttaaga gcaagataga ttatattttc tatattatta
    39541 taactcttac ttatattatt tggtcctata caaatagatt catcagctat atatctatga
    39601 aaacaatctt tatctatttc agaatagata gcaacagttt ttatacctag ctctttacat
    39661 gttctgatga ttctaactgc tatatcgcct ctatttgcaa ctagtatttt ttttatcaac
    39721 ttaaatacct cctactacaa tggttttatc ttaaataaag gttgattata ttctactagt
    39781 tcttcattac taactaatac ttcaattatt tcacctttaa tttcacttgt tatctcattc
    39841 ataagcttca ttgcttctaa tatacataaa gtgtcgcctt cttcaactac atcgccaact
    39901 ttaacaaagc tatctgcatc tggacttgga gaatcataga atgttcccat caaaggagct
    39961 actactactg atagattctc attagatgtg acaggtgtat cgtttacaac tggcttttct
    40021 aaagaaagat ccactactga ttcttgttgt acacttaaaa caggtgaact tgtagattga
    40081 gcttttttag atacttctaa tcttaaatcg ctactttcaa gttttacata ttctaagtta
    40141 gttgagtcta tggcctttaa taattcctta atttcattga tgttcatttt gttaccccct
    40201 tgtattatta cctggtgcta atattatatc acaaaaaaca aatttataaa agaaaaagtg
    40261 ctgactagag aatcaacaca tcaatatata tttttaattt taaattaaca accagtaact
    40321 tatatttgtt ttaattttcc attattaata taaattataa gtataaaatg aataaaattc
    40381 aatatttttt gcaaaataca agagaataca aattaatacc tcgggaattt ttaagacaaa
    40441 taccaatatt atgcaattta gacttcactt tctttcaaat cagtatcatt ttctttgttc
    40501 ttacatagga tacactttaa tttagctatt aaaatatcgc tatataggtt aaaagtaatt
    40561 agaattgcag gaattataaa ataaactcta gcacctataa aaccaaaaac tactgtagta
    40621 agtattaatc ttttatttaa agtaaaaaaa ggtatattga aaatactttt gtcggatgtt
    40681 tctggagtat aagaatatat aactccaaaa atcaaagtaa gcatacaaag tggaagtagt
    40741 gtataacttg aaattattcc cagaaatcct atactttcaa agtatttgct agcactattt
    40801 gtataaacaa acatgtatcc atttaagtag gcgcatatac atgatataat gccaagagtt
    40861 cctcctaagg attgagtgta ccaattaaaa aatttagatt ccatatacct cacctcctaa
    40921 aactgtatat ataatgatat gcatttaaat agaaaaatat atctttatat gcttatgaat
    40981 aaaattttgt taatttcatt aatttatgtt taagaaattt gaagtatagt gtagtatata
    41041 ttaatgtagg taaactttta tagtacttat tttatagtgc ttaatcaagt tatcttatta
    41101 taaatttgta tagatttaaa agttgttcat agggttttat attttgttga gctgaaaaat
    41161 ttattataaa tgaagggatg ttttcgcttt ggtagataca ttagaatttg gtcttaaaat
    41221 tttatttttt atattatcaa taatttggat gggtaaaata atgattttaa gaactgataa
    41281 gcaaatagta atcaatcctt tgcttattgg tatatctgca gtccttgtga tgttgcatac
    41341 ctcacagagt aacattgaat tttttggatt agatgtccag tatataagaa tagttttgta
    41401 tataatatat tcactgataa ttttaatagg aatatgggct actaacagaa gaaatggaat
    41461 tttttagata atctatttct ataaaattta aaggttgcta gtaacaaagc actaatatta
    41521 gcgttctgtt actaacaacc tttattttga ttatacactt aaattgaaaa tagaatgatt
    41581 gaataagttg acatattatt ttacattgac agtgattaga acttcttgag atgtatttaa
    41641 atcatttttg tcgcttagta taacttttac cacagcttga cctgttttat tacctttaat
    41701 tatcatatct tcacatgaaa gtacactctt atcacttaca gatacaataa agtctaaatc
    41761 ttttggaaga ttatcagtaa cttcccagta tttataataa ctatcaattt tcttcgtaat
    41821 tatttttctc aaatcaatat tttcaccttt tgctatatca acattatgat atttagagtc
    41881 cagtttatac tgattatctt ttgtatcaat aaaatttaaa tcgcaattta catccacacc
    41941 attattagaa agagaattta ctacaccatc atcaatcaaa taattattgg caagatttat
    42001 tgaatccata tcttttagac ttaaagcttc cttaggaacc tcttttatat tattattcca
    42061 aaaaataaaa ttgtctacat tttcaagata tgatatacct tttaaatttg aaatgctctc
    42121 accagttgca taaaattctc catctaaagc ctttatattg cttatagtca tctcatctgg
    42181 atttatatat ctttttacaa ctgctcttag atttgcatca ggaaaaatat ctttaagttg
    42241 attttcgctt aaaaccttat catttgaatt tattggactt aaagctgctg cagaaataat
    42301 aaatggtatt aaattagcta aattcatagt accctcccac ataaattttt tattataata
    42361 atttaatttc tatgagaggg ttctgtttcc ttgttatata tacctacttt cgtacacttt
    42421 attaatataa attaaaataa atttctacta ttaaacttat aaacatatta ctagttacgt
    42481 tttttagctt cacatctaaa tattcgcata ccttgtgaca taaatttgtc ttcatactct
    42541 gttgtaacat tattttcata ctcactattt cctaaatcaa gagaaatatt ctttaatagc
    42601 caattatttg cagcaatttc atttaaacta aactcgaata atttttcatt atctgtttta
    42661 aagtgaattt cgccatcatc ttccaaaacc ctattgtaca ttgctaaaaa tccagaatga
    42721 gtgagtcttc ttttactcca tctattttta ggccatgggt cacagaaatt tatataaatc
    42781 ctcgataatt ctttagcttc aaagtaatcc aaaatattac ttacatctcc ccataaaaat
    42841 agtatattat tcaaattaga agcatctgct ttttcaacag cctttaaaag aacttcttcc
    42901 ttaagttcca tggcaatata atttatgtca gggttctgct tagctaaagt tgttataaac
    42961 ttaccttttc cagtcccaaa ttcaacatgt attggattat catttctaaa ctttaaattc
    43021 cacttgcctt ttaatttgtc tatatcgtct cttaaaacat attttgtata acttaaaagc
    43081 ttttcatctg cacctttttt tcgtcttctt ctcacaataa ttctccttac atataaaaaa
    43141 ttagtaaaat acatttttat tatacattat ctcaccaaaa aataaaaaga actctcttaa
    43201 aatagaaatt tatttctata aagagagttc taaacttact aaaaacttaa tcctagtggc
    43261 accctgaaca accagagcat cctcctccac cttgatcttt catattttca ggaataactc
    43321 taaatccaaa tcctgtatct tctattatta tatctccata ctgactatat tcatcagagc
    43381 tcattacaaa ttgtaattct ccaacttcat aagttaagtc atcttctttt ttctcatcta
    43441 aagctaatcc aaaagaagga cctccacaac caacacctgc aaagtatact cttatattat
    43501 taggtttatc ttgattgtct tttaagatac tttttaaagt atcaatagct gtttctggta
    43561 aagtaatttt catcaaatca ttccctttca aatattttat taaaacaata tctattatat
    43621 acccatacaa ggtacataat caattagttt ttgcatattt tactaaaact tctacctaat
    43681 attacctaag gctttaaaat attttatata atattttatt accttaaata aataccctat
    43741 cataaactta gtaaacaaat aaaattatta ttctaaaaaa tataataatt ttatttgttg
    43801 ttttaatatt ataacacatt tgataatgtc caattcatat atgcaaaatt tatatacttt
    43861 aaaatatcta agaaattatt caataaaaaa atactatttt tttaaattat cattattgat
    43921 aaagctctac tatttagatt tttagtgtat tcataataag caaaagtaca acagtatata
    43981 ctgttgtact tttttgtaat tattaaacaa tattttggtc tataacaatg aaaaatggct
    44041 actttccata attgatttca tctgacataa gcaaaaagga tgtccagctg ggtcaatcat
    44101 aactctccag tcatcagtaa attgctcctc tgcgattgta gctccacaac gaattgcata
    44161 ttgaactgct tcttctaaat cattaacagc gaaatccata tgagccattt gctgttgagc
    44221 ttcaggtttt tctggccaca caggcggttt atactcagga tttcgttgaa atgttatacc
    44281 aggatacgtt ccctgatttg ttcctggagc accaacacat gcatattctt cattataaaa
    44341 tgctatttcc caattgagta acgctgcata aaattttgct aattcatatg ggtctttgca
    44401 atccatcgta aatgagtaca ttttgatttt tagttcatca tgcataacgt aattacctcc
    44461 aaatataaat ttcttcgtct tttcattttt aagctaaatt caaacattaa gtatgtaatt
    44521 tattccaagt gtttttctat tatgtcccaa gcctttgttt tttgcaatat aaagaaaaaa
    44581 ttatatattt taatatttta taattattga aattgtataa taaattcaac tggatacatc
    44641 gtacgttttg aaatttcttc taaagctaag ccttgtttta aaagcctttt tatgacactt
    44701 tccatacttt ctgaaatttc tataatattt ttatcaaaat cataaaacct aaaagttctt
    44761 tgaccccatg ggtattcctt tatataatgt aaaaaatcaa tattttctat tctatttatc
    44821 ttgctttccc attgtttgat atcatcaacc tcaaaataca actgaaagtt atttgattgt
    44881 tgatatattg gtaactcata tccaattata gctttataat tactttgtaa tccaaatcca
    44941 ttttcaaatg atacatgttc tcctaaatct aatgtgattt tttgctccat aacatcttca
    45001 taaaactttt ttgatttatt tatgtctttg acaattagca atactcctcc aaatttcatc
    45061 ttatcactat cctttctttg ttataagtat tataacttaa aattttgcaa tctcactgta
    45121 aaaatcagac atattagctc ggtatttttg aggagttatt ccacagacaa ttttaaaatc
    45181 tttaataaaa tgtggtatat catagaatcc taattcatta catatagaca tcaatgaagt
    45241 tttattattg tttagaagtt ttatagattt attaatacgt accaaacgag aaaatgactt
    45301 catactcaac cctaggtact gattaaaaag tctgtttaaa tgcctagaac tataaaatac
    45361 ttttttagaa atttcttgtg aagatatgtt tccagagttt tgaataatca actgaattgc
    45421 caaaataaac tcatatgagt attcaatttt aatattagaa atcaataatt cttccaactt
    45481 taaaatcaat ttatctatag ataaagattc attaaatatt tttttaattt ttaaatctaa
    45541 tgaagtatta attatagaaa atggaacaac tttatcaata agttcgcttt gctgtatacc
    45601 tataagcggg aaaaaaccag caggttgaaa ttctataatt agtataacat cacatctatt
    45661 agcaatacct cctactgttt ttggtttagt agttggacca aataatagac tatgtaatcc
    45721 tgtggcatca taaaaaaaca ccaaagttac acttccatga ggaataatcg tgtaattatc
    45781 agaaattatt gtttgatttg gaaacgatat agtaaaattc gatatccaat ttcttaattg
    45841 tatacaaggc aagagataaa caaaattgtc ttcacaaact agaagcgacc tatttttatt
    45901 tataaaacga atactttctt tttttaacat atattatatc ctatcctatc gtgaatattt
    45961 attaaagtaa gaagaatcca tactatctct acatatcatt aactagtttt aataataaag
    46021 atagaatgaa tactcattag atatttaata aacaaataaa attaaaataa tcaataaaaa
    46081 atatagtact cttatctgtt attttaatat tatagcacat ttataatatt taatttatat
    46141 ccactttaca tatgtctaat ttatataatt aaaaatgatt atctcaaata caataattag
    46201 tccttataca attaaaccgt ctgaaactgt aatagtacga ctactacttt tggctgcttc
    46261 tggtgagtgt gttaccataa ttatagtttg tccattgtcc ctgtttattt tatccagtaa
    46321 atctattatt tctatccctg ttttactatc taaatttcca gtaggctcat cagcaaataa
    46381 aatctctggt tttccaatta aagctcgagc tatagcaaca cgttgttgct gaccaccaga
    46441 taactctcgt ggtgtatgtt tacgtctatc agttaaacca actatatata agatttcatc
    46501 taattgattt ttataatctt ttaaattttt accatctaat agcaaaggaa gcattatatt
    46561 ttcctccaca tttaaatttg gtattaagtt ataaaattga aatacgaatc ctatatttct
    46621 acgacgtatt atgctcattt tctcatcatt aaaatgtgat atatcagctc cattcatata
    46681 cacttttcca ctcgttggag tatctaaacc acctaaaata tataaaagag tacttttacc
    46741 agaaccagat tgacccatga tagaaacaaa ttcacctttc attacttgta aagacacctc
    46801 ttttaacact cgagttgttg tatttccaat tttatattct ttgataatat tttttgcttc
    46861 tacagctaaa ctattatgtg taattttcat attaacctcc ataattattc aaacttaatt
    46921 tcttctatca gtttcatttt cttccctttt ataataggca ctatcgaacc tattaaagtt
    46981 actataattc ccaataaccc aacaataata aatgttttaa aatctaaatc tggtttcatt
    47041 gaaatttttg gtcctgcaac tataaagata gtttgaatct caagtataga tattacaata
    47101 ccaataacag ctccaagtaa acctgaagta aacccttcaa tcaatgtcac ttttatattt
    47161 tgtttattac ttagtcctat agatttatac atagctatag aacgtctttt ttgaatataa
    47221 ttaatcagta gattattaat aatacctact gtagcaagaa gaaaaatgaa ataggtcatc
    47281 ttattcatag gagacaaaaa actacttata gtatttaaag aatcattatt aaattcttca
    47341 actgtacgac tccaattata tgtatctcca aataaatccc ttatctgaat cataacagca
    47401 tctggattga ctgctgtata tactaaaaaa ccataattag ttttatcaaa atcattaact
    47461 gcataatgtg atggtataac agcttctaca tcatttgcac gagatttaaa actacctact
    47521 attttataag aaaaatcata cttatcttta gatagagtta ttgtatctcc tatagataac
    47581 cctacttttt ttagagtatc ttcattcaaa ataacagaac gtttatcctt aaaagcttct
    47641 gttacttgtt tttttatctc aaaatttgta taattgattg cgaacataga gttatatagt
    47701 tctatattat ctgtaccctc tagtcttgat aatgttacac cattaccaga aatctcatta
    47761 ttcataacat aaagaggtaa tatattttta attccatcca tatgtctaac atcttcaata
    47821 aactcttcat tcatctttcc atctgcaaaa ccttgtaatt ctgcgtcacg aaacacatct
    47881 gttatataag ttttgacaaa gttaccaaca acgcttattg caataactgc agaaatactt
    47941 ataaatagta aagtgatatt ctgtattata tttttgttat ttttcatatt tctagcagct
    48001 aatttacctt cattccctaa aatattttta taaacaaact caaaaactat agacatgata
    48061 tcagtaataa gaggaatcaa cacaatagta gctacaatta atcctagtaa tgaaaatccg
    48121 ccagctagat acaatgtatt ttcaggggat attcttggaa gtaaaacaga taaaataaac
    48181 attatacttc ctataaaaag tatagaacga tttgaaacat tcttttcttc tactgttcct
    48241 agtacaatat tttttattgg caaatggcta gcttttttaa caggtatata tgcactaaat
    48301 gaagaaacga ttattgctac tataacagat ataatcactc caatgggtga tattactgtt
    48361 ggaattgata ttccttgttc gagagaattt cctaacccat gaagcattaa atttaaaaca
    48421 actacaccaa taggaatagc aattaatcct cctatacttc cgtataatat actttcaagc
    48481 atcaatatat gagttacagt tttttcattg gcaccaatac ttctaaatgt tccaataaaa
    48541 ggaagacgtt ctaatgtaat aaccttataa ctgctgtata tgataaatat actaagggtt
    48601 aatgcaaaaa aacttattaa aaagaaaggc attgtctttt gtctagcatc tgaaattatc
    48661 ttagtttcat ttatagtatt tgataccgta tatttttcag tagaaagttc ttcggataat
    48721 ttatttacta gattttctgt catttcttct tctgactcta taagaactct tgtatatcca
    48781 ctgcctttat ttataatttt tgatagagtc tctttaggta gcagtgcatt cacacctctt
    48841 gtatgcctca aaaatactgt atcataagat gcaatatcac taacttgaaa agtataagac
    48901 tttccataaa tctgtaaagt aatagaatca ccttttttta ttttatattt tgatgtaaaa
    48961 cggtttggaa gaataatttt atcaccagaa aaatcagtaa tactatcgcc attttctaat
    49021 cgtggcttgt tgattttatt tagttgactt aagtctgctg ctataagact aaattcttca
    49081 taatatcccc ctttattata aatagcagag ctttccaata caccaacctt tgattttata
    49141 gcatttaaat caggtatgtc ttctaaattg gttaatatat ctggattttt actttggaca
    49201 gatatagtcg ctgtaccaga catacctttt gccatttttc tttgtgcact ttcataagat
    49261 aatccaattg agaatgaaac aaaaagtaaa actgttgata aaactatgga caataacata
    49321 actgctgttc taatttttcg ttctttaata tttgtgaaaa tatactttaa tataattttc
    49381 aaatttacac cttcttcctt tatctaagat atttcagtat aatattttta tactttaatc
    49441 caaattgaat ctctaataga ctcctccata cttttatcat tctatttata atatttttaa
    49501 tcaaatcacc tccttaaata aaaaagattg ataaactact atataaatat aataatctat
    49561 caatcttaat atatttttta tttgacctta attaacctta attaaatagg aaagatgata
    49621 taaaatgaac tacccaatcc aagtatactt tcaacagata tattccctcc atgaacttca
    49681 ataatttgct ttgttattgc tagtcctaaa ccagttcctt ctattttttg ttttgtattg
    49741 cttcctctat aataccgatt aaacaaattt gatgcctctt ctcttgacat cccctttcca
    49801 ttatcagata taatgatttc aattctttca tttaatgtta tatgtgttat atgtacacaa
    49861 actttagtat ttttatctcc atatgtaaaa gcattaacaa taagattact aaaagctcgt
    49921 tttaatagtg tataatcaaa atcaaagtta atattatcta tattgctctt aaattcaact
    49981 atatttgatt catattctgg ataatttaaa atatcaatgg ataattcttt caagagtctt
    50041 actaaatcat agttattttt atttaaaggg attattccat tttttaattg ataggttaat
    50101 tttaaatcgt ttataagtgt atttgtatat tctatgttct ttaacatgat tgaagcatat
    50161 ttatatactt tttcaggtga ataattagta ctactatctg taagaagctc tgcataccct
    50221 tttattggtg aaagtggagt ttttaaatca tgagttatat ttgaaatcca ctcttctctc
    50281 ataatatcag ttttattttg tatctcatca cttttatgaa tttcttcatt taaaatattt
    50341 aagctatcat atatatcagt aaaaacacct ttaatattaa ctggaagata gcttcgtttt
    50401 gaaatatcac taacagcaga tgtaatattt gatatgcact ttgttaccca gtaaccataa
    50461 gcaattccag aaaccaaaat tattaaaaat ataatcccta tgattgaaaa tacaattgac
    50521 ttaccaccta caaatttact tccatcaaaa aacatattca ctttagatat atttaatgga
    50581 aaatatataa tataaatata gtcattttta ttatttttaa ttgtattaaa gtatacagtt
    50641 ccattacttg aattattttg gttagctaat gttaataatt cagaattaga ataataatct
    50701 ttatgctctt ttggttctaa aaacccataa actctatttc cactagaatc taaaatttgc
    50761 aaccacaatt gattttctcg aagcttttct aatccagact gttttacttg tggcttatta
    50821 tctataaata cgatttgttc attaaagttt tctgtaaatt ttttcggcca gtcactacga
    50881 ataaattccc catttggttt ctgaatagta ataagtgata agaaaatacc tattactgtc
    50941 aggattaagc aaataaaaat aataaaaaaa ataatgtatg aatgaaatac tgttttataa
    51001 ctaggtttat gcatttaatc aactcttttc ttaaactttt attttagttt gattgttgct
    51061 tatataaaat agacaataat tattgacatg taaaaattca tttaaaaata caactgtcta
    51121 cataaattat atttatataa ttatatatta tcaagctgtc tgttgtattt aataaatcct
    51181 tttgataata tatacaaagt aagataaaat tttccattaa ataccttata attatacaca
    51241 aaatttttat atgcgttaaa tagtatagag aaatttttca tagaaccatc tttatagggg
    51301 atactttata aaagaattta aaaattataa tatataaaaa acttcatagg agtgtaacaa
    51361 catcagtaaa aatatgaaaa cactatataa aaattcaaaa atattaagaa taatgaaata
    51421 cttgaaattg cttatatcaa gaaattttat ataatataaa ctggtcaaat atgttataat
    51481 attaaattgt gacaagttca tggaggtgtt tatatgaaaa aacaaaagtc gccaatttta
    51541 cttgaaatgc ttggactatt tttgggttgt atatcaatga gtattggtat aaatatgttt
    51601 ttaaaaccac atacaattgc tcctggagga cttagtggtc tatcactagt attaaacaag
    51661 gttacaggac ttccagtatc agctatcatg ttaattatag gtgtcccatt agtaatactt
    51721 gcttttagaa ttatgggtac aaaaaattca ttaaaaactt tatttggaac agttgtattt
    51781 tcagctatag tccaattaac agacccatta tcaaagctaa gatttactaa tgacttatta
    51841 ttgtcttcta ttgcaggagg aatcctagtt ggtataggtt taggaataat gtttaaatca
    51901 gatgcttcta ctggaggaac agacttaatt gcactcatac ttagtaagaa attcccagga
    51961 ataaaagcta caaaattcat gtcatgccta gatggaatgg ttgttatatc ttctggtata
    52021 gtaaatcgta gtttagaaac tgcactttac tctggtatag cactttgtgt attaataaaa
    52081 atagcagata tgataatgga aggatttgac cattcaaaag cattttttat tatttctgat
    52141 gagcctgaaa gtttaagaca agctataact gaagaacttg atagaggtct tactattctt
    52201 gatggtaaag gtggatatac tagagaaaat aaagaagttc tattagtcgt agtttctaaa
    52261 aaacaagaac tttatctaaa gagattagtt aaaagaactg accctactgc atttgttatt
    52321 gttacagatg tgcatgaggt tcttggagaa ggatttaaaa atttagagaa ctaataaaaa
    52381 attaatattt taagtatata tactatttta attaaaatac gatttattca aaaagagtgt
    52441 ctcataaact ttttagttca tgagacactc ttttttgtat aaaatcaaat gcatttttag
    52501 tattttaata ataaaaaaca ggcttatcta tattttgagg caaccacttt attgaaattt
    52561 tatttagaaa acaatcatta ttaaataact agcataaata ttaaatgatt atatctattg
    52621 ttttttcaat tcatattgat aacaaggaac attttttaca gtatttccag tatgaatttt
    52681 tttgatatac tttcctgtac atttaaatcc taataaatct aatgattttg tagaattttg
    52741 gttttcttca taggttattg caattatttt atttaagttt agttctctaa atccatattg
    52801 tattactctt tttgttatct cagtagcaaa atcatgcccc caatagcttg gatttataca
    52861 tatacctatt tctgcttcta atgctttagg atttatatta ttaaacccca tattcccaat
    52921 aactttattg tctcttttat atactatagc aaagtgtcca attttccctt ttttataggg
    52981 atttagaaaa aatgatttta taaactttct tgtatcttct aaagatttat gctcactaaa
    53041 tggtaaatgt tttactacta ttttattttt ataacagtca aaaaaatctt cgacatcatc
    53101 taatgatgca ggtcttagta tacaatttct agtttctaag gttttcattt gaggatattc
    53161 agaatcagac atttctaatc cccctt
//
LOCUS       DAEORZ010000005        52897 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEORZ010000005 DAEORZ010000000
VERSION     DAEORZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.005"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            15..914
                     /locus_tag="KRH39_000241"
     CDS             15..914
                     /locus_tag="KRH39_000241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510172.1"
                     /translation="MLKNNGKVTHRKANGESSSIPGWENIISIASYDNSIIGIKKDGT
                     VVSKGLGNSDSDNISKWRDIIDIECDSGTFIGLKKDGTVVATGNNDYGQCNVNNWFDV
                     VSIEITKSTIEYMKNTTVIGVKKDGKVLLTGYDSLNHRKETDKWRDISKIKCGSYGIF
                     GIKNNGTVVVSGSAYDIKNWKDIVDIHIGLCGIVGIKRDGTIISTDEKFNEIIKNWKD
                     IISAEIIDNHEGISIVGINSNGDILTSSRGQNLDNIKRLEDVEYINFDDSIDNFLVGI
                     RKDNVIFIRGINNTINEEYNEEI"
     gene            948..1304
                     /locus_tag="KRH39_000242"
     CDS             948..1304
                     /locus_tag="KRH39_000242"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510173.1"
                     /translation="MKNYIARCLSNMGVLLAGIQVLAGIIIMCQKVEVPYDYLSDTYE
                     QYSPIMIGIGAGVIVSGIVICLVLKGLAELVENSADQTQYQKEILMLLKNNNVNEKIN
                     EVKIEQQFTNEELPKL"
     gene            1513..2226
                     /locus_tag="KRH39_000243"
     CDS             1513..2226
                     /locus_tag="KRH39_000243"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012301.1,HMM:NF016296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8510174.1"
                     /translation="MIITICENDDMQRDIIYDFVKIFLTKIKVKHYDILMFKNGEELL
                     EEYPNKVDLILMDINLGGRNGFDISKDIRKFDKEARIIFVTVSEKYAIKCYEINAFRY
                     MIKPIYYKEFEKYMLDFAIDWRKREAIITIKRKKQIIKIEEHNILFVEIVNRKLRVVT
                     KNEEHIINDKMDMFEEKLKEDIFFRCHKSYLINLLEVTNFNYNFVYLTSEKIKIKVAP
                     KKTKKLKEKLANFFREIIW"
     gene            complement(2690..3043)
                     /locus_tag="KRH39_000244"
     CDS             complement(2690..3043)
                     /locus_tag="KRH39_000244"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8510175.1"
                     /translation="MNENLKELRIKLGLSQKEFAEKLLVSDATISRLEKGERKLTDRF
                     IFQICEVFNVNEDWLRNGNGEMFKNIDDIELAAMMGKTFSSNDEFLKKVFLTFAELTD
                     DEREVIQKVIDKLSK"
     gene            3223..3393
                     /locus_tag="KRH39_000245"
     CDS             3223..3393
                     /locus_tag="KRH39_000245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510176.1"
                     /translation="MYNNNDKKLINLFESFKKIDYTEQSLVYFLSLSFIVNRKCHKDA
                     NLYSRRGECNYE"
     gene            3386..4192
                     /locus_tag="KRH39_000246"
     CDS             3386..4192
                     /locus_tag="KRH39_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBG8510177.1"
                     /translation="MNNLNIKGKQKFMDLEIPIIEGGFGEGKKCITDKTISEIHNMEI
                     KHVRELINRNIRRFINAIDFIDLKVVVDNDYNLEVLELLGYRKMQISKANNIYLLSER
                     GYSKLIKIMDTDLAWEIHDKIMDEYFNMREQLIDSYMIQDSIERAKRWIEEEKERLLL
                     KETIQVQQPKVEYHDKVLNPEKLVTTTDIAKDLGMTAQALNKLLHLNRIIYPERINGR
                     IKCWKPYKNYLWLIPEYADFKITEHDTQLKWTEIGRKWLIEFVENIKKIS"
     gene            4256..4480
                     /locus_tag="KRH39_000247"
     CDS             4256..4480
                     /locus_tag="KRH39_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510178.1"
                     /translation="MIKQILNKRGSLLNLASEYLTEQDYLKFQGWYTHRYSKMDAYDA
                     SINEYLNYKNDKKLITYVFNLKFQEALIEI"
     gene            4504..5040
                     /locus_tag="KRH39_000248"
     CDS             4504..5040
                     /locus_tag="KRH39_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510179.1"
                     /translation="MNYNYVTKIDLKYGNTQFGFEKYSDALDKYRELKEEFKYVPCII
                     KLHSYVAHQDLYVKEIKADGFEKKYLDIKNLIKLFKEHKQNISDELSLCDKKKEEVLH
                     VIEEMNLDTASLNDKIDTIKELKNTLAKRRLVKYELNKYFAFCNTLYEIEEELKKYES
                     KYKPKENQGKSRFKKNKK"
     gene            5049..5189
                     /locus_tag="KRH39_000249"
     CDS             5049..5189
                     /locus_tag="KRH39_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510180.1"
                     /translation="MEEYQFNLIKKLCAERKIHKGWFRNIEKCGCGGKCNKKNCKMLE
                     KL"
     gene            5200..5433
                     /locus_tag="KRH39_000250"
     CDS             5200..5433
                     /locus_tag="KRH39_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510181.1"
                     /translation="MENQIELTEKDIENTLSALNIIKYICTKSKNCKNCPLGYMKNLD
                     EHNCMLYELIDKGLLPCDWSVKKVTRLLDTTEE"
     gene            5452..5862
                     /locus_tag="KRH39_000251"
     CDS             5452..5862
                     /locus_tag="KRH39_000251"
                     /inference="COORDINATES: protein motif:HMM:NF021182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510182.1"
                     /translation="MELDFRIWNGFRMIHGEELRLLTEDMFIKSGYSEQYHNHICIPR
                     MEDLKVMQCIGLKDCFGKKIFQYDIIKFIFDGVWKISLVDLSLIKGTTVTFESIGGKY
                     TFNLSEIFSKDRKLEIIGNFYENKDVIEKYNLSI"
     gene            5876..6070
                     /locus_tag="KRH39_000252"
     CDS             5876..6070
                     /locus_tag="KRH39_000252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510183.1"
                     /translation="MDEMIIRVEMGEPLPTLGKIIKCTTCLTNEEKCFKVNKICKLSW
                     INNRNSEGLIVECKGKYIKY"
     gene            6277..6819
                     /locus_tag="KRH39_000253"
     CDS             6277..6819
                     /locus_tag="KRH39_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBG8510184.1"
                     /translation="MSKKDRLSVFISLILRHKPETIGIKLDDYGYADVNELIEKINNT
                     GRNINIEILEQIVKEDNKQRYSFNDDRSKIRANQGHSINVEVELKELEPPKCLYHGTA
                     TRFLENIKKEGIVRQSRLYVHLSNDIDTAVKVGKRHGVPVVLKINTGKMYENGYKFYL
                     SENNIWLCKYIPFEYVEIFE"
     gene            6963..7229
                     /locus_tag="KRH39_000254"
     CDS             6963..7229
                     /locus_tag="KRH39_000254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510185.1"
                     /translation="MKYEEYEVALKRIEEFEEITEYKQIADEVLEKLDSNNCILKIKF
                     DFGDCLTYDLNLSSDDEQENFIKKEMYCAFRNIKNKLEEMMEEI"
     gene            7340..7591
                     /locus_tag="KRH39_000255"
     CDS             7340..7591
                     /locus_tag="KRH39_000255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510186.1"
                     /translation="MDLRCISLHFYYKIVDLEKFEDVFYSQTNFKEVTGDIDKITSLE
                     TFKYYSDYYRKIIAKGLEIDENKVISVSCEEYYENTYED"
     gene            7638..8414
                     /locus_tag="KRH39_000256"
     CDS             7638..8414
                     /locus_tag="KRH39_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510187.1"
                     /translation="MENIELTPRNYFEDIKNRKQKNTNENLDIVYENSLILLNKYIIT
                     GQKKGAKKLMFLLECIEKERNVIELGIDTFVYKEDIEKYIDDISKNTVKIIELENYER
                     EIPDEIVEIIEKTKDIFDKMYVLFTDYTGKITKEISKERRDKDPILFGVFSDEKSRAI
                     VDRFYYLGDWEDEYCDLTLEKMINDYKKKGRQDILKHIITPKSLDELKKELDSLEKNN
                     DTYKQLANIKPLSTTKNSSVEPPKLNIFKKIKNRVFKNEK"
     gene            8404..8808
                     /locus_tag="KRH39_000257"
     CDS             8404..8808
                     /locus_tag="KRH39_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510188.1"
                     /translation="MKSNVDLTENRDFRAVRGRGINILGIEDAPNRMLNGFSDFGIGR
                     LYGKNLEFEFNKNEVFLTENANDREEKKFLILCEENKVCVRCGDELKEGWDKVSIELC
                     NKCYEMLDVEYKNNKIIRELFFRDRDVNIELL"
     gene            8846..9235
                     /locus_tag="KRH39_000258"
     CDS             8846..9235
                     /locus_tag="KRH39_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510189.1"
                     /translation="MKDVWLYSWDDECFRSDEYESKEEAVEAAKEELKRLGEIKRWAY
                     IGKKEEADIPDIDAKDALECIQDRIDDEYGEYGEDWFENIRAEDILILESRISEVFKK
                     WIDDFKYKPYWFAVRDIEEIELKNAVN"
     gene            9295..9615
                     /locus_tag="KRH39_000259"
     CDS             9295..9615
                     /locus_tag="KRH39_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510190.1"
                     /translation="MFIKGFRCICRHKFSRIKDCYLITTKSQQYTCKIWVCKYCGQIV
                     AGSTPVYHPNMKVNEYCVKCELLACANNYEGYCNSKDKEIEFKSVCLDDGEQFLRCEN
                     FDWE"
     gene            9635..9820
                     /locus_tag="KRH39_000260"
     CDS             9635..9820
                     /locus_tag="KRH39_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510191.1"
                     /translation="MKINAYVKTNKIGSGCEFEFEIDDDNLKNMSDEEKKDYIDKIAW
                     DYVFESFIDWNWYEVED"
     gene            9864..10856
                     /locus_tag="KRH39_000261"
     CDS             9864..10856
                     /locus_tag="KRH39_000261"
                     /inference="COORDINATES: protein motif:HMM:NF013623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG8510192.1"
                     /translation="MNKDNLETIIEGYKVFNPDWTCRNFKYEVGKTYEHDGDIEVCEA
                     GFHFCQKAINCFNYYSFDNKNKVAKIEAIGLIKTDGDKSVTNKITIVKEIKWEELLNI
                     VNIGNNNIGYGNTGDYNTGSYNTGDYNIGSYNTGDYNIENCNTGYRNEGCCNEGCWNT
                     GNYNIGNCNSGDWNIGDYNSGVFCTDVPKIRMFNKETDLTYEDWINSEARNILKRNSY
                     LTKWIHIDDMTEEEKENNPGYEINNGYLKVFTFEEMCKNMWNNLTDEEKKIIIDIPNF
                     DADIFKEITGIEVIKLKIKECNIVDKQEYTNCCGNCKECDEYHKMLCNSLYEEE"
     gene            10859..11122
                     /locus_tag="KRH39_000262"
     CDS             10859..11122
                     /locus_tag="KRH39_000262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510193.1"
                     /translation="MAKYDLLLKLNNILFKMEKHCDEMIKNNNDKELRTCTNCEFAYY
                     GSYVESGCVCTDIEGFGIHDMVKRIIKKNKRKKNKKMEYRKNY"
     gene            11139..11402
                     /locus_tag="KRH39_000263"
     CDS             11139..11402
                     /locus_tag="KRH39_000263"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510194.1"
                     /translation="MNIQEDIEKWDKFETPIISKEVDFYLLKIGFQYGRNDGRNTLVG
                     IKDMRHISISKVNSNLLSIDIHKFIKQENGKLSEFKAHEYIEK"
     gene            11456..11857
                     /locus_tag="KRH39_000264"
     CDS             11456..11857
                     /locus_tag="KRH39_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HBG8510195.1"
                     /translation="MKELLEVKKIFDSLASTSSRKEKERILEKYKNNRMFVECLQFLL
                     DSNILTGISKNKICKNLNNTSHNELENIYDMLDYLIKNNTGRNIDVKTIQVFASKDEK
                     LKDFIFNLATKSIKLGITYKTVDKIMPGLII"
     gene            11959..12462
                     /locus_tag="KRH39_000265"
     CDS             11959..12462
                     /locus_tag="KRH39_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510196.1"
                     /translation="MTDKIIIASVDGEEMIFRTEMKKLEKGDSLIVSVNGVKRLALFI
                     RYISTCEEYIEDIAFQKVDDILYENTIAKEKERVKKVFKDKCMKNYKPIVNTEIDSYL
                     RECGFNCDGRQFFKSIDNTQILISRDCDKDVLTVSKGLTITKTDITNKNLITVIKIIN
                     NSLLIVR"
     gene            12522..14747
                     /locus_tag="KRH39_000266"
     CDS             12522..14747
                     /locus_tag="KRH39_000266"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBG8510197.1"
                     /translation="MKNVVKRDGRIVPFDEKKIINAIEKSFLSLRNKGKSEVFDRELA
                     TLIAKKIKNKPCEKMHIEDIQDIIEFSLMASKKKDVAKEYITYREKRNIARGRKTYND
                     YMSIVNTESNDITKENANMNADTPAGMMMKFASETTKTFTDHVLLSEEAKEAMMDNYI
                     HIHDKDYYPTKSLTCIQHPLDKIFNNGFRAGHGSSRPTKRIETSSILGCISLETVQNE
                     MHGGQAIPAFDFYNAQSVRKTFIEEIKNIEIKDDIDLSHLYNIEIEDYIIKDLKELDS
                     EKRNVQHAINRTVWRVHQSMESFIHNMNTIHSRGGNQVVFSSINYGTDTSAEGRCIIR
                     EILKSTYEGVGNGETPIFPIQIWKVKEGVNYNPTDRNYDLYELACKVSAKRFFPNFLN
                     LDATFNKHVKWKMCDPNRYIYEVATMGCRTRVFEDLHGEKTSIGRGNLSFTTLNLVKM
                     ALEAKEKYPNNEANRVAEFIFLLDKYSDVVARQLYDRYLFQCTAKAKQFPLLMSGLWK
                     NSENLNPNDDVSEVLKHGTLGVGFIGLAECLIALIGEHHGESEKAQKLGLEIISLLKS
                     KVEKYKKKYNLNYSVLGTPAEGLSGKFTQKDKKIFGEIKNITDKDYYTNSSHVPVWYN
                     CNVEHKAKIEAPYHELEGGGHIFYVELDGDATHNPKAIMKVVDLMKKYNMGYGSVNHT
                     RSRCMNCGFENADSELKECPKCGSNSINIIQRITGYLVGTTDSWNNAKLAELKDRVTH
                     G"
     gene            14740..15237
                     /gene="nrdG"
                     /locus_tag="KRH39_000267"
     CDS             14740..15237
                     /gene="nrdG"
                     /locus_tag="KRH39_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBG8510198.1"
                     /translation="MDKDYIYILDIKHDTIVDGEGFRTSIYCSGCNHMCRGCHNPQSW
                     DIKNGTLTKVLDIYKEIISNKFSDVTFSGGDPLLQLQGFVHLAKLIKKNTNKTIWCYT
                     GFKFEELITDNEKLELLKLIDVLVDGKFEEDKKSLELIFKGSSNQRIIDVQKTLYEKQ
                     IVLYE"
     gene            15438..15845
                     /locus_tag="KRH39_000268"
     CDS             15438..15845
                     /locus_tag="KRH39_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510199.1"
                     /translation="MYFETDIIKNKNTVNKIIKYCGGNTEKLKDWLKEIENKSISSLE
                     ELKEYPLKRNYKFVFSKEEAPNLDKLIKMKLRDNCLEKCEICSLENLLSMLNDEILDL
                     NKEDISYEDNIEWIENCKEELIKLKVGSICIDW"
     gene            15866..16402
                     /locus_tag="KRH39_000269"
     CDS             15866..16402
                     /locus_tag="KRH39_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510200.1"
                     /translation="MRFWCENCNKYFNAEETLQEYNYFLNEEILICPSCKKDLIPIAR
                     ETELSLGFDSDTNQLTYVEYDSSDYSLLRKVNADVEDVVKPIIHYIRNLNKNSLDLNG
                     ITITMNGNREGKRLDGLNYEEGVVMDNLINAWNGFCKLKRQHPSELGDFQNAIHQAQQ
                     VLGLRVLRNDYPKGWIKK"
     gene            16423..16821
                     /locus_tag="KRH39_000270"
     CDS             16423..16821
                     /locus_tag="KRH39_000270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510201.1"
                     /translation="MSTKTILKNIDIKDDKAAERLISALEESENKQDSDLDIKKIFEL
                     IDNSISSYIIYGTPKALSKFESILNIFKIDKANSQHVQKFIKVPAIDLGKYCDYKNNK
                     ALFDDNTVYIIPKDTKLKIYKDEYKYVVLK"
     gene            16976..17134
                     /locus_tag="KRH39_000271"
     CDS             16976..17134
                     /locus_tag="KRH39_000271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510202.1"
                     /translation="MLKEGTYCVSIEGKLSSEEVELGNVTITMFVDGNIILKMDVLKY
                     LVSIKLNR"
     gene            17159..19201
                     /locus_tag="KRH39_000272"
     CDS             17159..19201
                     /locus_tag="KRH39_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510203.1"
                     /translation="MEFNNFKKQVQERFNKMTTGISYIYEVDLDKDRLWNLYLDSFPE
                     GTNEIFRERREYDCSCCRQFIKNIGNVVTIKDNKISSIWDIDTDCPTFKPVVKALSEY
                     VKSHNINDIYVSKFKKIGTDKNFENTSEKVYEWNHLYIELPDRFVDETNKSVGDIKGR
                     IRDVKNVFKRSLDEISEDSVSIVLDLINQKSLYKGEEWQNPLIEFLKYKRNYIKLQTE
                     TDKQNFAWEQSVKAGVVIGKIRNHSIGTLLLDISEGVELDKAVSRYEKIVAPSNYKRP
                     KAIYTKKMLDEAKKTITELGYMDSLQRRHATIDDITVNNILFVNKDSAKQMGEFDIFD
                     EMSKGIGIDSKKFSKVEELNIEEFIQNVLPTSKEVELLLENRHIDNFVSLIAPVNKEA
                     KSMLKWDNNFGWAYAGNMTDSSMKENVKNAGGKVDGVLRFSIQWNDTEFDNNDLDAHC
                     IEPNGNHIYFGNKTNYSTTGMLDIDIMHPKRGNVAVENITWSDINKMRLGTYKFYVNN
                     YSKRSGISGFRAEIEFNGEIYSFDYNKPLRDGENVMVAEVTLDIGNRFKIKPLLPSNV
                     SNKEIWGLTTNQFIPVSVIMNSPNYWDGQQGIGNKHYFFMLKDCINEETVNGFYNEYL
                     KNDLNVHRKVFEALGSKMYVPFSENQMSGLGFSSTRRNEVIVKVRGSYERILKIKF"
     gene            19226..19606
                     /locus_tag="KRH39_000273"
     CDS             19226..19606
                     /locus_tag="KRH39_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510204.1"
                     /translation="MENVFEMATRNKFRFEFRGLISVEDLWDLKLEQLDEVYKTLTTD
                     MKKANEESLLKIKTKDQKEVELKIKIVKYIVELRLAEIENRNKEKKKKEQKQHILELI
                     NKKENQELENKSIEELKSMINSLE"
     gene            19684..19905
                     /locus_tag="KRH39_000274"
     CDS             19684..19905
                     /locus_tag="KRH39_000274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510205.1"
                     /translation="MDSKIEVGSIWGLEDGIAQPQYVGTKVKVVSISYIEEFNKRLIT
                     FMEIEEKEEDTWIDADWEDVFLNVFFKLD"
     gene            20148..20378
                     /locus_tag="KRH39_000275"
     CDS             20148..20378
                     /locus_tag="KRH39_000275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510206.1"
                     /translation="MYNKIFKEIIIDDIYRVNLAEGRFIRMCDHLEEYIMILIKDKIE
                     EQYFKNDKFKEIRQEIIRFEKKGQVTIKLKDE"
     gene            20465..20620
                     /locus_tag="KRH39_000276"
     CDS             20465..20620
                     /locus_tag="KRH39_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510207.1"
                     /translation="MYSKGDVVKVWCYQCEKIEYQEFVGYFNSYFDNYKLKKRWKCSK
                     CGVINNF"
     gene            20647..21093
                     /locus_tag="KRH39_000277"
     CDS             20647..21093
                     /locus_tag="KRH39_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510208.1"
                     /translation="MKIKKIILGLDNLENVELGIGDVGEFYLDNIHEIIGGFALSYIG
                     KCKKADYLHIQIHKRVDEIQRLHDYKDIINVKIEYEDGSVEDIYVPWKQEGDFLGVDN
                     LYQTSFISKQGYLHIVISKDKTVEEVFNEEFEDDERVEFSFEMYGI"
     gene            21118..21582
                     /locus_tag="KRH39_000278"
     CDS             21118..21582
                     /locus_tag="KRH39_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510209.1"
                     /translation="MGLDLGFYSVKTKVTDLDTYLNKTRVQKSKECKTVEDFYEFEEL
                     LDIDIALENWSIIRMIYKAKWEKGYESYEDEAILITKEDLEDYIIPFLKQKDINSKIY
                     NKDKEYYYKNDKKVTVDDRYTQEFWDLVLKEFQKMLNIIDFEKHSLIIMYWY"
     gene            21611..22864
                     /locus_tag="KRH39_000279"
     CDS             21611..22864
                     /locus_tag="KRH39_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HBG8510210.1"
                     /translation="MEVQGKYNKAKIFTENVDSITIGQIINLCNQKEFKESSIRIMPD
                     CHAGKGCTIGTTMTISDKVVPNLVGVDIGCGMATIPLEKYINNINYTKLDKIIRNDIP
                     HGFKIHNKSREKLLKNLFNIDINNLRCEVNKNRAYMSLGTLGGGNHFIEVDKSEKGQM
                     YLTVYTGSRNLGKQIADYYQDKAIKYCVEKYKKSYDDAKEFLISTLKKDNKEHLINEY
                     LKDLSDRKVNKPHDDLCYLENDLMEDYLHDMIIAQKYAAANRIVIIADILFKYNSPDV
                     NYDHFIRDIKCDVIECIHNYIDMDSKILRKGAISANKDETVIIPINMRDGIILGKGKG
                     NSEWNYSAPHGAGRILSRGKAKEKISLDEFEESMKEIFTTCVGQSTLDEAPQAYKPIE
                     DILNNIGDTIEIMEVLKPIYNFKSN"
     gene            23232..23411
                     /locus_tag="KRH39_000280"
     CDS             23232..23411
                     /locus_tag="KRH39_000280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510211.1"
                     /translation="MRLDDSQIGLIMYMIGFVHYDKEQFQFYMDTIYGKEYSEKEIVN
                     MVEHLRQDIKIQRFK"
     gene            23433..23585
                     /locus_tag="KRH39_000281"
     CDS             23433..23585
                     /locus_tag="KRH39_000281"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510212.1"
                     /translation="MSFRCGGIAYADGIDVGVGFCYEPLHCDCGWSEMCAYEDKEEFD
                     EWEYKK"
     gene            23602..23964
                     /locus_tag="KRH39_000282"
     CDS             23602..23964
                     /locus_tag="KRH39_000282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510213.1"
                     /translation="MGHYSEEWEECAFCKTNSNKCDVYDKLFVSTPICEKCATEITKI
                     IEENKRRDTIDIRNMHIESINNLFHDWDINSKLTYKDMSTIIELLNEVKNRDNYENIT
                     TVDEYYGLNIKSYRKRRF"
     gene            24096..24380
                     /locus_tag="KRH39_000283"
     CDS             24096..24380
                     /locus_tag="KRH39_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5052 family protein"
                     /protein_id="HBG8510214.1"
                     /translation="MKRFLLIGVLGTMLIGMSGCTGIQSSMKDAESDVSGLNRVVNVY
                     SDNGDVLKTYTSKNMRVKDGDGGTITLDFDGKRVIICNAHVVIEENQSNK"
     gene            24392..24559
                     /locus_tag="KRH39_000284"
     CDS             24392..24559
                     /locus_tag="KRH39_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510215.1"
                     /translation="MFKVLKTILIIIWVVDILNFPQFQFLDTTYPINTLAWLLIWILI
                     PSSSIYINKEE"
     gene            24738..24887
                     /locus_tag="KRH39_000285"
     CDS             24738..24887
                     /locus_tag="KRH39_000285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510216.1"
                     /translation="MIGKLNFIEISKDISDDELNDLLVKHPDAVMFVDEEMITRWNKL
                     KNKDK"
     gene            24899..25504
                     /locus_tag="KRH39_000286"
     CDS             24899..25504
                     /locus_tag="KRH39_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510217.1"
                     /translation="MFISSTNRSNVRDFHISDYYRTPVIKIKEFLNEFSKYEDIFNSK
                     IKILDPCAGGDNTNPMSYPTAIQEFSNQDISIDTVDIRDDSRANIKQDYLKFQPKEKY
                     DVIITNPPFNLSLDIINKALNDVREGGFVIMLLILNYLGGKIRQELWKNNMPKYIFVH
                     NKRISFTDDGKTDSIEYAHFVWQKGYNPNFSQLKVLISQKN"
     gene            25612..25857
                     /locus_tag="KRH39_000287"
     CDS             25612..25857
                     /locus_tag="KRH39_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510218.1"
                     /translation="MDIKIDMCLLEMEINRRRCEYAARTGRYPNSLILWEGYYSLLNI
                     QYIEDKTRGYITKFNDMDVIKCNDFLKIELFEKNINN"
     gene            25886..28957
                     /locus_tag="KRH39_000288"
     CDS             25886..28957
                     /locus_tag="KRH39_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG8510219.1"
                     /translation="MRYNNYHSHKIYSNIRSLDVITKPQQYIDRAIKLGQNTYFTTEH
                     GYQGNVYEAKTLCDEYKLKMIIGAEFYYVNDINEKDRGNYHLIVIAKNNDGYKQINKA
                     LSLANKNGYYYKPRIDEKILFEIFKPGDVVITTACVAGILKLENREELILKLKNYFKN
                     NFFLEVQSHPHKTQALHNKDVLELSNKYNIDIIHANDSHYIYPEESKYRTKFLKAKGI
                     NYPEEDGFILDYPNSEDIFKRYEKQNVLTKQEVERALKNTLIFDECEEITLINDDIKL
                     PSISKNPNKELKEILNKEWLEKRKNIPKNRWNEYLDAIRYEFDIIEKTHMEDYFIIDY
                     KIVQRAKQEYNGLLTKTGRGSAPSFIITNLLGLTEIDRLNAPVPLFPTRFMSVERILS
                     AKSLPDIDLNAEDAEPFIQATRDLLGEENCAWMISYKPLQDASAFRLWCKANDMKLSE
                     YDEVAKNLDKYTNDVFWKDVIKESKVFVGVVESVSFSPCSMLIYDRPIDEEVGLLKTK
                     DGVCCNIDGYYCDKYKYLKNDYLTVKVWSLIRKTCELANISIPTIEELNILLDAKTYD
                     IYKDKLTCTINQVDSDYATNLASKYKISSVAETSAFVASIRPGFASLLDNFINRESYT
                     TNVKELDILLEDSYHYLMYQESIMKYLIWLGIEESESYDIINKIKKKRFKEKELKELH
                     IKLKNNWIKKVGTENNFEDTWQVVNDAASYSFNASHSLSYAYDSLYCAYLKSHYPLEY
                     YTVAFNLYNEDTERTRKLTDEIEYFSIKLENPKFRFSKSEYFFNRDTNSIYKGIESIK
                     FLNSDIGEYLYSLKDNKYDSFLELLIDLQGHINSKQLSILIKLDFFEEFGKSNKLLET
                     YDIYNSIYSKKQFKRDNLPCNINTMRKYSNKETEKIFKEVDTKELCSYLESQIENIDI
                     PISEKIQAHFEFVGSCNIKDDNSNPRYCLVIDIDTKYSPKITLYNLKSGNTKIFKVSK
                     SIFEDNKIDIYDLVYLKSTKEKAKSKKVDEKWIKSNTETEWWVQEYWKVEN"
     gene            29060..29311
                     /locus_tag="KRH39_000289"
     CDS             29060..29311
                     /locus_tag="KRH39_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510220.1"
                     /translation="MISILIDEQRHRDLLEKNRELNTKIDELNEEILFLKEENKEYEE
                     VLEKWKNVKEEIKSAYEIRRRLNRKYGKLEMIWRKIEDL"
     gene            29311..29631
                     /locus_tag="KRH39_000290"
     CDS             29311..29631
                     /locus_tag="KRH39_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510221.1"
                     /translation="MNTNKIYEKKEYIIIKSKNGYIVYNTNKKFEEGHTHLKSYNAAK
                     TAIDLVIKRRIPRSYSIYFLTSLMRISNDVQYIKHISDIVYIRNSKGLKEKYINSKNI
                     KKNY"
     gene            29703..30077
                     /locus_tag="KRH39_000291"
     CDS             29703..30077
                     /locus_tag="KRH39_000291"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510222.1"
                     /translation="MLIKGDKIKLIKEVDGLRVFKVGDIFTVINIYNDGTIHFKTDYG
                     MGFMSYSEFKEHFEIANNKEKRKYTWSNWTTIVSPNLHILYTYKTNNKKVIVKKDGVK
                     ASATCHDTDVFNLQNSAESPLL"
     gene            30149..30550
                     /gene="tnpA"
                     /locus_tag="KRH39_000292"
     CDS             30149..30550
                     /gene="tnpA"
                     /locus_tag="KRH39_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG8510223.1"
                     /translation="MENKYRRTQTTVSLINYHFVFCPRYRRKIFNIEGLEDRFKTLVK
                     NKCKEMDIEIVAIECDKDHTHMFLNSLPTLSPSDIMQNIKGYTSKILREEFIELSKMP
                     SLWTRSYFVSTAGNVCSETIKKYVENQKTRY"
     gene            30568..32007
                     /locus_tag="KRH39_000293"
     CDS             30568..32007
                     /locus_tag="KRH39_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG8510224.1"
                     /translation="MSNYILTLQLKTEKFQEDIICNAFDKYRKVYNSCLSELFKRYNH
                     MIESKEYQANCKYKGKDRNKIFSEINKKYNLTEYSLHKFVKSIGKYYKLHSAITQKIA
                     TRCFNAFSKYIYHQAKQVNYIRYSELTSIEGKQNSTGISYKDGIIKFNKMTIPVIIKN
                     NDNYAQRAIQDKIKYCRILKKEIKGKIKWYVQLVLEGIPPKKATNQGKIKGQIGLGNV
                     GIDIGTRTIAISSKSEVKLLELAPNVNNIDRQIKLIQRKMDRSKRATNLNKFNDNGTI
                     KQGNRDKWIFSNHYLKLKKLRKELYRKQAEIRKQDHYILINKLLNLGNKFYVETMSYK
                     RLQTRVKDTTINEKTGRINKKKRFGKSLANKAPSMFLTMLDNKLKYNNERLYKIDTAK
                     CRASQYNHFSDEYNKKELKDRWNNGMDIQRDCYSAFLIMNVNEDLKSINRELCVKTYD
                     NFKILHDKEINRLKKLKLNGYKLISSMGI"
     gene            32363..33256
                     /locus_tag="KRH39_000294"
     CDS             32363..33256
                     /locus_tag="KRH39_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510225.1"
                     /translation="MAEKRINYNQVFVSGEVLDILNIARMNQGTGQEAIRFTLKVETA
                     PNESVNVDYFTSMFKSDGSPNQMFLGIETIANEIKTRAEDGKGDIVRCVCSLDNNLYY
                     KDGEKKERFQISGTFCNREKYDDKGNPIDDKGEVVKSIKASQVWRVYTLIENIEEKED
                     DLGKYLEITGLINKYGKQGCNMTFRIHNEDMIEGFKSLYKIGDVGLLEGTVKSMVTSK
                     SAGFGSRIKKSAFTFLEIEGGDEPLKDGDEILTDKNYPFTDKNIEAMREKIKEKNEKE
                     KQRDIDKNGKTVEVSDDDVPF"
     gene            33296..33853
                     /locus_tag="KRH39_000295"
     CDS             33296..33853
                     /locus_tag="KRH39_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBG8510226.1"
                     /translation="MEVKEEIMSDDFKKVVNEEIKKYISNLHSDRIDGLKESDSQIEI
                     TKALIAFHLDIESIPKDNTNPFFRSKYANLDTILKAVNPLLAKHDLILMQSAEDAGND
                     KVYIKTKLKHSSGEYIESNSAPFKPAKTNDIQARGALETYLRRYAVQSVLALSFEEDD
                     DGNSLTNKSKSKSEEENSTVKKSRV"
     gene            33915..34448
                     /locus_tag="KRH39_000296"
     CDS             33915..34448
                     /locus_tag="KRH39_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510227.1"
                     /translation="MNCKCKVCKKKLNTKDAYKVEHITSGGNKQNRYYCNEQEYRKEQ
                     QDIYFWKQCQLGIDYIMGYTVISNQKNKMLQEIIKNGYTREELYDCMLEKKDEIIELL
                     NYRKDIEEEYPKLCYVFTILKGCIRDITIRNKQIKDEKENEKIYKESEKYYEVITPKK
                     VLTNKRKSLFEKIKEVD"
     gene            34448..36022
                     /locus_tag="KRH39_000297"
     CDS             34448..36022
                     /locus_tag="KRH39_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510228.1"
                     /translation="MEAYEKITKDRGIIETLVLGTMLKSLTLFSEYKISESDFIIDKV
                     KFFFSLGRTMSKTHSELDEASVAKFVSSNKLKSEYEKYGGWNSLSSAMEYGKETNISA
                     YIDDLAKNNLLIALDKRGFNITKEMEHNGLKFIPFELFQSMNCREVEEFYEGLISSCS
                     VNSIKNNMKVENLLLTKEDREKLKQKTEAGTPYNIIFEYSEKETGLSDSEESKYIYGL
                     PVLSNRTNGLGKGGGINIISGFSGIGKTTLLFFNYILAMIYQGEKVVIFANEQKSQYF
                     KSMLVSFIAYNIFNYHDLDRNKIDNGDFTDFEEELMEKIEIFLKNRGFEESLKFIYME
                     EFEISEILRKSKELVAHEGFTVIAVDTFKSENSSDANYTGRLIENSKLLDNFGNKYNV
                     ITLLSMQLVTAQENKSSYLSASDLAEAKAVKNVCGLLMLMRKVVNDLELDSTNKKFFL
                     KPYRLKYNKLKKTTEREYIQFDVKDLQKEYRLLFLNKSRRGQDGDVILLRFYGKTGRF
                     EEIGRCEKVYRGQLSY"
     gene            36032..37096
                     /locus_tag="KRH39_000298"
     CDS             36032..37096
                     /locus_tag="KRH39_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510229.1"
                     /translation="MEIKDLTNEQVINFMEYLGSDLSPKSNDRQLIFNTCLCHNGDSY
                     KLFYYTESKTFHCYSSCGHIGSLIDLLIHINKYDFKDAVNEIKNFFGISNQPMLRKGF
                     RIKKKLKQDDIRDIQIELLPTSKKPYVYKIFQQIPIEEWERENISFEVLKTYQIHYNP
                     YENQIVIPHFCWHDKDRPVGIRVRNLDEDKAKSFGKYIPLWYDSKCYNHRLSLNLYGL
                     NINEQAIKKRKKVIVFEGEKSVLQMATMYKNNPSVAICGSNFSREQKKILLDLGIEEL
                     IIAFDRQFKTKGDEEYIAWKNKISKLVQDIKDVVSISVIWDEDNLLDYKDSPSDKGKK
                     IFEKLLKNRVNIIQFEGVIK"
     gene            37093..37863
                     /locus_tag="KRH39_000299"
     CDS             37093..37863
                     /locus_tag="KRH39_000299"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015340.1,HMM:NF021206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HBG8510230.1"
                     /translation="MSEVIRQVVDEIVRISSLEVAKMMGMTSKDGHRDLIRKIEKINI
                     VFDSEKIPNEIYWLEGEYENRGKFYKEYLVSKKGCEFLAHKTTGEKGVLFTAKYMERF
                     EEMERIIKEQKLELSARDKGILDIVNSKSDIEQAIAIKTFEEVVTAPLIETIEEQKPD
                     VQFANRIRTDGRKVYSISQASKLLKLPYGRNKLMENLRNLKILQLNNEPYQTYIDRDY
                     LLVELNETGNKLVVSPRITGKGMKWLENKLRDKLLTLA"
     gene            37878..38132
                     /locus_tag="KRH39_000300"
     CDS             37878..38132
                     /locus_tag="KRH39_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510231.1"
                     /translation="MIQEVREEVLLQNGDILHTGDIINVEYKTMEDTKEQFCKGRLKS
                     VDEQFIQLDTSEKYNAAEKTIYAWDLIEIKKVGDENEKDN"
     gene            38116..38373
                     /locus_tag="KRH39_000301"
     CDS             38116..38373
                     /locus_tag="KRH39_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510232.1"
                     /translation="MKRIINSKYRALYLFMFFLIFNLIYSLYFGRNTEIGFYSSTSCI
                     EEYILDIITSIGLFFSMMLAGFDVTENFINSLRDYCEEDAK"
     gene            38384..38593
                     /locus_tag="KRH39_000302"
     CDS             38384..38593
                     /locus_tag="KRH39_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510233.1"
                     /translation="MELKDYISKVELNDMPLTLDNQYMLNEISISEYGKIFAVKDTSG
                     RTSYYTEIEFNKLFRGINSILENII"
     gene            38667..39509
                     /locus_tag="KRH39_000303"
     CDS             38667..39509
                     /locus_tag="KRH39_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBG8510234.1"
                     /translation="MEKELYSFSKLNAFHTCPFQYYLTYVKNLNREQNCYGYYGNELH
                     SLLEQLQQRKITNQEAIQKYNEVIEYANLMDYNFPTPNSRINYLECILHYIENFVPIE
                     CDKYYIEEYFEFDINGITMRGYIDLYYIIDNKVYVIDYKSSSKFSKKDLPKKSMQLIL
                     YAMYLKEKYPDKIIEYVAFDMCKYVENEKGTLIERNRIGNIDDYERAIVKIKYTKALE
                     QQLIAFVTDTVKQIKQLDFNDESVWCKSDDKSNQFFCKTLCSHYNKGCKHKENKSFRK
                     SKSQ"
     gene            39544..39840
                     /locus_tag="KRH39_000304"
                     /pseudo
     CDS             39544..39840
                     /locus_tag="KRH39_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            39857..40438
                     /locus_tag="KRH39_000305"
     CDS             39857..40438
                     /locus_tag="KRH39_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBG8510235.1"
                     /translation="MDKLKNIFVFLGYSGSGKDSIVTEISKQFNIPILISHTTRPPRN
                     YLEIINKTYHFVDNKFFKKEKNNFIEMRKYIVHDGSTWLYGIHKSELENKKYALTIVD
                     ASGYKALEKYFTGTKTKLVPFFINANEDTIRKRLIDRGDNLKEIERRLVDDKIKFKEF
                     LNSENYIVIANNLSITNAVEQVKIHMKEGIQWS"
     gene            40429..40923
                     /locus_tag="KRH39_000306"
     CDS             40429..40923
                     /locus_tag="KRH39_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBG8510236.1"
                     /translation="MVLALDVSMSSTGWAVINRNKNILKYGKIVTKKDKFKSEDERMC
                     YICNAIQELIITHNIQIVLIEDQFTSRNSKTILGLRKLLGAIMRTVKLNNIEIEYMYP
                     VSIRKYLMNSGKAKKEEVAAYVRENIIDIGEYIDRTCKAKTSDIYDSIALGVAYLNKV
                     GDVI"
     gene            40932..41384
                     /locus_tag="KRH39_000307"
     CDS             40932..41384
                     /locus_tag="KRH39_000307"
                     /inference="COORDINATES: protein motif:HMM:NF025107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8510237.1"
                     /translation="MYNLKAVKFEELTKKYKLEVAPYREMDSNFFLVCRGIFVLKDSK
                     AMHLYLYLCNCYNRATEKVFPSYDTIQRATGLNRNTVSNKLKILQKLKLIKINKRKVG
                     TGFNNYYLINYIKSVIEKSKINENLEIEEIIDMDNCLADLKLDTSIQN"
     gene            41558..42064
                     /locus_tag="KRH39_000308"
     CDS             41558..42064
                     /locus_tag="KRH39_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyuridine 5'-triphosphate
                     nucleotidohydrolase"
                     /protein_id="HBG8510238.1"
                     /translation="MSINVKIKKLSQDAIVPSYAKEGDSGIDLYTLEDTVIPAKSAVA
                     ISTGIALEIPYGYEMQVRPRSGISLKGILFTRDVTVCDKEQLKKQIRKSFVVHPTVRL
                     GTVDSGYRGEIKIITYNEENFEILIPKHIKLAQGVFQQVPRAILKVVDELSNSERGNS
                     GFGSTGIK"
     gene            42228..42401
                     /locus_tag="KRH39_000309"
     CDS             42228..42401
                     /locus_tag="KRH39_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510239.1"
                     /translation="MYRYDDYVNLINDLELPKEIRNKCVRVIMTLDNMQGIMVIHKFK
                     TYVVNEKYIGKVA"
     gene            42497..43912
                     /locus_tag="KRH39_000310"
     CDS             42497..43912
                     /locus_tag="KRH39_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG8510240.1"
                     /translation="MKLPKKLLSLGLVITMLIISFPKQSYALNSVDEIEVSTIYETAG
                     LIADKQSYDTAILVNLNDSIADGLSASGLSGTLNAPLLLSENSSIPNETMKRLSKVKT
                     VYLIGGIKSISKSVENTLMSKGFNVKRIDGDDRIKTSYNVAKEINSKQKVNTVMLTNA
                     YKGEPDAISIASVAARDKAAIILTDGKSIPFSTNEVKSYAIGGTSTMNDTLVKNTNST
                     RLGGSDRFDTNKKIINQFYNGIKEFYIAGSRNLTNALLASSLKSPVVLVDNNSNKSIL
                     KNATKITTVGDITEELLEECLNVTNGIGDSNTGVIKKTRLEEAKELAKDWYNCYEVGD
                     IDNVPYDGVYLVDDNYKNGSNYYYVFSYVSAGQEHSDRLCINKNDMSDYKLLYEDLHM
                     EKVVSFKNAEDLVKDLFMKEHGFLPNHVEAQNMEDGMYIYVRPFNTYVDMNGIEHNAG
                     GYTPYLVNIYTGEISKYTYTE"
     gene            44034..44783
                     /locus_tag="KRH39_000311"
     CDS             44034..44783
                     /locus_tag="KRH39_000311"
                     /inference="COORDINATES: protein motif:HMM:NF019976.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HBG8510241.1"
                     /translation="MFKEIFEKVFMSEEVSKTVKEVNNIIKDTKIQVKGGFGELRLDF
                     ILSRLGKDYIIIKDIIVPGSNKTTQIDSIVISIYGIFVIECKNFSGYIYGNDKDKVWT
                     QIVGKTKNTFYNPVRQNYAHIKAIENIIGDKYNIYSIVVFSDKATLKNVMISGNNIRA
                     INESEILRTISRYKDMTIDKEEIKIIRDKIFDCMKNTNQNTREHVRNLKNITEEDKCP
                     RCQIGHLIKRKGQYGEFWGCSEYPKCKYTRK"
     gene            complement(44851..45408)
                     /locus_tag="KRH39_000312"
     CDS             complement(44851..45408)
                     /locus_tag="KRH39_000312"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510242.1"
                     /translation="MYCKNCDLEFDDTTTICPKCKSVLKSNKNTYQKNFENKINEFKL
                     RKKIRQGNILSTITILTVAIHILVELFMQIHIEDYYEIYIKYSPSAISVLVLVLSLKR
                     GEKRHQFLNNILFISVMILISIFGTIYFSAPLVNRIFNGNYLFKEYWTVYGVGNFIII
                     LECILIIFDDSYYLSYINKKENSNK"
     gene            complement(45415..46425)
                     /locus_tag="KRH39_000313"
     CDS             complement(45415..46425)
                     /locus_tag="KRH39_000313"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510243.1"
                     /translation="MKKTKLKVLSLTLATTFLISTTVNSYTISAKSSDMDMNTVADVL
                     TVSEEYSNALKDIGISVGELKDLTPKGSDFIQKCNSYLEKNMDIRKTYSEEPIKHTTN
                     ASQLSYAMSAARKNQEVGAPKYNDEVVYMYLSHFVDFEGDNYEEGLCTYITKYDREAY
                     DRYLSRGSAFRSANSMRSVITNLIALKGDLPSLYTTLAKEKASVVKTIVDMKSFEIDV
                     QSVKSMVENDMFIFFKDAYSRHDTPEAIIEDVQGSMKIKYKDYNEQIGYMVLGLVFSA
                     LTFTPPVMAMLAEVNLYSMVIKDIFDKANYAALQYSRSARKAERFNLWMEENGMYMEK
                     KA"
     gene            47004..48116
                     /locus_tag="KRH39_000314"
     CDS             47004..48116
                     /locus_tag="KRH39_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG8510244.1"
                     /translation="MNAFIRKRNKNYVVYLEFRDDESGKRKQKNMGAFDKKRDANKRL
                     AEVKDSIYKDSFLVPNEITLSEFLLDFLEKYKDNISASTYKSYIAICKNHINPSIGKY
                     RLQELRNIHIQNYIDDLAGNLNPQTIKVHINVLRLAIKRAYRIKLIKENIIDGIESPR
                     IKKFKNEIYDKEQMLKLLEVAKGTNLELPISLATGLGLRLSEVLGLTWDNIDFDENTI
                     TVSKITSRLDGSVMLKEPKTESSIRKIFAPVELMNLLKDYKLEQNKKLLRSIIRNEHN
                     LLFFDRKGNPIAEDVMSKKFRKFLENNDLPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNINTTLNVYSHVLKEMDKEASDRISENLFKAN"
     gene            48161..50005
                     /locus_tag="KRH39_000315"
     CDS             48161..50005
                     /locus_tag="KRH39_000315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510245.1"
                     /translation="MGTSKISNIHKENLSIAEIQKLCAIAGNIEANIQSNDKTPSWDG
                     ELFLYEKNKIEKENCLDNKKENLLRKINIQLKANEVKKLSGKKRTFSMDVSDLRNYYN
                     NEGVILFVVEIKSVNKIKVYYRNLLPVDLINILNEIDRTKKNQLTKSVELYELRPENN
                     HFERIVNSFYRNINKQTKNLVDKQIELSERDKEISIDIAEIDNRYDLFNRSVYAYKPL
                     KHEDLDTIDLPCVNKLYLKELNTKTIVDIFIKNNIYKNNEYISTVNKTNHKITINNFI
                     VIYIYRILDEKLINKSIDINFTIKEPSGTVDSHLNNLKMLLDIFKHKKVLIKEFSTKD
                     ELIDLDLKTMPCEEKDLIENILFFEDFKNLLEMLEIKQENFLLDNMSQEDYGKINTLI
                     SIFINNKTIKKKNMSKHSGFAYMKINEKKIILYKIETDNSWNLINIFDKKNNLTITQR
                     EGDMQCSPYILLNYNDMDSINFDVNKATINIKKYKKTPEYTDRVMSYILELIKYYDKI
                     LIKEYLIEALELLEWIENEVDVDINKINKYQIIKRIRNFSIDEILDLDNIKRKNSKDI
                     MIQCAINILIEKYEEAKNNMNNMSKELLNKFKLYPIYNLYDKKTKVQI"
     gene            50530..50907
                     /locus_tag="KRH39_000316"
     CDS             50530..50907
                     /locus_tag="KRH39_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8510246.1"
                     /translation="MYLRKLSKSELIVMKFIWNLDTKVKSYQIINYMKEKYSWSEKTT
                     LKTLSKLSNKRFIYVQETSQCTYYTISIKEDKYHEFISQKIHKLLGCNSIKSLLASLF
                     QEELTDEKITSLEEWVKNWEEEE"
     gene            complement(51522..52169)
                     /locus_tag="KRH39_000317"
     CDS             complement(51522..52169)
                     /locus_tag="KRH39_000317"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510247.1"
                     /translation="MSLKKRVTKIISLALIGVIASLTLLTTTVNAQTNNINKYYTSEK
                     STESYTDKNLGVIYDRVVDGDKTTITIKSLDGNVLNTLEDIDNEVYLDGKKISITSSE
                     NYKSNDNSLEQSEIDTFKAAVKWGKWQSNTMYINTGGLGTATISALIALKAPWAPVKV
                     AATVAATVAGKYDQIKIYYKIRYGSDGKYNYYERETTFYGDGKYITKFTDKGKRY"
BASE COUNT        22613 a         4847 c         8846 g        16591 t
ORIGIN      
        1 aagataatgt atatatgtta aaaaataatg gaaaagtaac acatagaaaa gcgaatggtg
       61 aaagtagttc tatacctggt tgggaaaata taatatctat agcatcatat gataactcaa
      121 taataggaat aaaaaaagat ggtacagttg tttctaaagg acttggaaat tcagattcag
      181 acaatattag taaatggaga gatataatag atatagaatg tgatagtgga acttttatag
      241 gattaaaaaa ggatggtact gtcgttgcta ctggaaataa tgattatggt caatgcaatg
      301 tgaataattg gtttgatgta gtaagtatag aaataacaaa atcaaccatt gagtatatga
      361 aaaatacaac agtaataggc gtaaaaaaag atggaaaagt attacttaca ggatatgata
      421 gtctgaatca tagaaaagaa acagacaaat ggagagatat atcaaagata aaatgtgggt
      481 cttatggcat atttggaata aagaataatg gcacagtagt tgtatcaggt agtgcttatg
      541 atattaagaa ttggaaagat atagtggata tacatattgg attatgtgga atagtgggta
      601 ttaaaagaga tgggacaata ataagcacag atgaaaagtt taatgaaatc attaaaaatt
      661 ggaaggatat tatttctgct gaaataatag ataatcatga aggaatttct attgtaggaa
      721 ttaattcaaa tggagatatt ttaacatcta gtagaggtca gaatttggat aatataaaaa
      781 gattagaaga tgtagaatat ataaattttg atgactcgat tgataatttt ttagtaggta
      841 taagaaaaga taatgtaatt tttataagag gaataaacaa tactataaat gaagaataca
      901 acgaagaaat ataaaaataa atattttaat ttggaggaat agaaaatatg aaaaattaca
      961 tagcaagatg tttatcaaat atgggagtat tgttggcagg tatacaagta ttagcgggta
     1021 taataatcat gtgtcaaaaa gtagaagttc catatgatta tttgtcagat acttatgaac
     1081 aatatagtcc tattatgatt ggtattggtg ctggggttat agtaagtgga atcgttatat
     1141 gtttagtttt aaagggatta gcagagttag ttgaaaattc agctgaccaa acacaatatc
     1201 aaaaagaaat attaatgttg ttgaaaaata ataatgtaaa tgaaaaaatt aatgaggtta
     1261 aaattgaaca acaatttaca aatgaggaat taccaaaatt ataaaatgtt ttaaattata
     1321 tactaatact cagtactaat taaagtactg agtattttta tgtaacagta aattatatta
     1381 atataaaaat atattctaca tacataaaat gataaaataa ccatttataa tacattaaat
     1441 tacattaaat caaaaaagta tatgtcgaaa acatataata ttaaataaat atatacttta
     1501 aggagtgatt taatgattat aactatttgt gaaaatgatg atatgcaaag agatatcatc
     1561 tatgattttg ttaagatatt tttaactaaa atcaaggtaa aacattatga cattttaatg
     1621 tttaagaatg gggaagaact gttagaagaa tatccaaaca aagtggattt aattttaatg
     1681 gatataaatt taggaggcag aaatggattt gatatttcaa aagatattag aaaatttgat
     1741 aaagaggcaa gaataatttt tgtaactgta tcagagaaat atgctatcaa atgttatgag
     1801 ataaatgcat ttagatatat gataaaaccg atatattata aagagtttga aaaatatatg
     1861 cttgactttg caattgattg gagaaaaaga gaggctatta ttactataaa aagaaaaaaa
     1921 caaataataa aaatagagga acataatatt ttatttgttg aaatagtgaa tagaaagtta
     1981 agagttgtta ctaaaaatga agaacatata ataaatgata aaatggatat gtttgaagaa
     2041 aagttaaaag aggatatatt ttttagatgc cataagagct atcttataaa tttacttgag
     2101 gtaactaatt tcaattataa tttcgtttat ttaacaagtg agaaaataaa aattaaagta
     2161 gctcctaaaa agaccaaaaa acttaaagag aaattagcaa atttttttag ggaaataata
     2221 tggtaaatta aagaaaaatt tctataatcg aatatatgtt cttgataaaa gtatgtgtac
     2281 aagatataat attattagta ggaaaatagg aaaaatatta atatttttga acattttgac
     2341 attaattgtg attttagtgt tgaatttctg ttaaaaaaat cttattattt aatcatagaa
     2401 atctactgtt atagagtaga agattgttaa ttataaatga ttttaaatta tctgagataa
     2461 aattttttgt ttctgaaaag aacgaatgtt cgattgaggt gtagagtgag aggatgttta
     2521 ttaaaagata ttagattatc ttacttaatt cataatgcat atatgggggt tctataataa
     2581 tgaaaaaaca agtcacaaaa gaaatactta aaatgctaga aaaaatggat ttatcttatt
     2641 tactcataat taaaagtttt gtatctaaat taaaatagcc cttattgggc tatttactaa
     2701 gtttatcaat tactttctgt atgacttctc tttcatcatc agtaagttca gcaaatgtta
     2761 agaatacttt ttttaaaaat tcatcattac ttgagaatgt tttacccatc attgctgcta
     2821 attcaatatc atctatattt ttaaacattt ctccatttcc atttcgtaac cagtcctcat
     2881 ttacattaaa aacttcacat atctgaaata taaatctatc tgttagcttt ctttctcctt
     2941 tttcaagtct agaaatagtt gcatcactta ctaataattt ttctgcaaat tctttttgtg
     3001 agagacctaa tttaattctt aattccttta aattctcatt catatttaca cctcctatta
     3061 tatagtatat taataaaaat tgacaatgtc aataaaatag gaatgataaa ttgcaaaaag
     3121 caaaaaaaat aattgacaaa tacagaatgt ggtactacaa tgtaaatata caaaacgcaa
     3181 ttaataattt gacaaacgca aataaataag gaggtagatt atttgtataa taataatgat
     3241 aaaaaattaa taaatttatt tgaatcattt aaaaaaatag actatacaga acaaagttta
     3301 gtgtattttt tatctttaag ctttatagta aacagaaaat gtcataaaga tgcaaatttg
     3361 tatagtagga gaggtgaatg taattatgaa taatttaaat atcaaaggaa aacaaaaatt
     3421 tatggattta gaaataccta ttatagaagg aggttttgga gagggtaaaa aatgtataac
     3481 agataaaact atatcagaaa tacacaatat ggagataaaa catgttagag aattaataaa
     3541 taggaacata agaagattta taaatgctat tgattttata gatttaaaag tggtcgttga
     3601 taacgactac aatttagaag tattggagtt gcttggttat agaaaaatgc aaatatcaaa
     3661 agcaaataat atttatcttt tatctgaaag aggatattca aagttaatta agataatgga
     3721 tactgatttg gcttgggaga tacacgataa aataatggat gaatacttca atatgagaga
     3781 acaactcata gatagttata tgatacaaga ttctattgaa agagctaaaa gatggataga
     3841 agaagaaaaa gaaagattat tacttaaaga aacaattcaa gttcagcaac ctaaagtaga
     3901 atatcatgac aaagtattaa atcctgaaaa attagttact acaacagata ttgctaaaga
     3961 tttaggcatg acagcacaag cattaaataa attactacat ttaaacagaa ttatatatcc
     4021 agaaagaata aatggtcgaa taaaatgttg gaaaccatat aaaaattatt tatggttaat
     4081 acctgaatat gcagatttta aaataacaga acatgatacg cagcttaaat ggactgagat
     4141 aggtagaaaa tggttaatag aatttgtaga gaacattaaa aaaataagtt aaatcaagaa
     4201 gttaaaatgt accttgaaaa gtagatattt aaaaatataa aagataggtg attaaatgat
     4261 aaaacaaata ctaaataaaa gaggtagtct attaaatcta gcaagtgagt atttgacaga
     4321 acaagactac ctcaagtttc aaggatggta tacacataga tatagtaaga tggatgctta
     4381 tgacgcttct ataaatgaat atttaaatta taaaaacgac aaaaaattaa taacgtatgt
     4441 atttaattta aaatttcaag aagctctcat agaaatataa tattactaaa catggtgatt
     4501 gaaatgaatt ataattatgt aactaaaatt gacttgaaat atggaaatac acaatttggg
     4561 tttgagaaat atagcgatgc tttagataag tacagagaac ttaaagaaga atttaagtat
     4621 gttccatgta ttataaaact tcattcatat gtagcgcacc aagatttata tgtgaaagaa
     4681 attaaagctg atggttttga aaagaaatat ttagatatta aaaatttaat aaaattgttt
     4741 aaagaacata aacaaaatat atcagatgaa ctaagtcttt gtgataagaa aaaggaagaa
     4801 gtattacatg taatagaaga aatgaattta gacacagctt ctttaaatga taagattgat
     4861 accattaaag aattaaaaaa cactttagct aagagaaggt tggttaaata tgaactaaat
     4921 aaatactttg ctttttgtaa tacattatat gaaattgaag aagagttaaa aaaatatgaa
     4981 tctaaatata aaccaaaaga aaatcaagga aaaagcagat tcaaaaaaaa taaaaaatag
     5041 gtgattagat ggaagaatat cagttcaact taataaaaaa gctttgtgct gaaagaaaaa
     5101 tacacaaagg ttggtttaga aatattgaaa aatgtggttg tggaggtaaa tgtaataaga
     5161 aaaattgtaa gatgttagaa aaattatagg gaggtacata tggaaaatca aattgaatta
     5221 acagaaaaag atatagagaa cactttaagt gctttaaata ttattaaata tatttgtaca
     5281 aaatctaaga attgtaaaaa ttgtcctctt ggatatatga agaatctgga tgaacataat
     5341 tgcatgttat atgaattaat agacaagggt ttacttccat gtgattggtc tgtaaaaaaa
     5401 gttacaagat tgttagatac gacagaagaa taaaacaaag gagctggtta agtggagtta
     5461 gatttcagaa tttggaatgg atttagaatg atacatggag aagaattaag attactaaca
     5521 gaagatatgt ttataaaaag tggatattct gaacaatacc ataatcatat ttgcatacca
     5581 agaatggaag atttaaaagt aatgcaatgt ataggactaa aagattgttt tggaaaaaag
     5641 atatttcaat atgatattat taaattcata tttgatggtg tttggaaaat aagcttagtg
     5701 gatttatctt taataaaagg aacaacagtt acatttgaaa gtataggtgg aaaatatact
     5761 tttaatttaa gtgaaatatt ttcaaaagat aggaaattag aaatcatagg taatttttat
     5821 gaaaataaag atgtaatcga aaaatataat cttagtattt gaataggaga agaatatgga
     5881 tgaaatgata attagggtgg aaatgggaga accattacca acactaggaa agataatcaa
     5941 atgtactaca tgtttaacta atgaagagaa atgttttaag gttaataaga tatgtaaatt
     6001 aagttggatt aacaatagga atagtgaagg gttaattgta gaatgcaaag gtaaatatat
     6061 taaatattaa taagaataat gaagttaaaa tcaagatttt aatagaaaag gagtaattaa
     6121 aaatgaagaa aaatccaata agaattaaaa aatgtgactg tggtggagat atatatgaac
     6181 ttaatacaca attaggtact atgtatcaat gtaaaagctg tggcgagatg tatagatagt
     6241 gctaaaacat tagttaagga atatggggtg aagtaaatga gtaaaaagga taggctatct
     6301 gtttttatca gtttaatatt aagacataaa ccagaaacta ttggaattaa actagatgac
     6361 tatggatatg cagatgttaa tgaattgatt gaaaaaataa ataatactgg tagaaatatt
     6421 aatatagaaa tccttgagca aatagttaaa gaagataata agcaaaggta cagttttaat
     6481 gatgatagaa gcaagattag agcaaatcaa ggacattcaa ttaatgttga ggttgaattg
     6541 aaagaattag agccgccaaa atgtctttat catggaacgg ctactagatt tttagagaat
     6601 attaaaaagg aaggtattgt tagacaaagt agactgtatg tacacttatc aaatgatata
     6661 gatacggctg ttaaggttgg gaaaaggcat ggtgtacctg tagttttaaa aattaataca
     6721 ggaaaaatgt atgaaaatgg ttataaattt tatttatctg aaaacaatat ttggttatgt
     6781 aagtatatac catttgaata tgtagagatt tttgaataga taaatcaagg tattaaactt
     6841 ttagaagctt taaaataaag gtgttttcag tagaaaatat taaattgtaa aaaaataggg
     6901 tactaaaaat gattaacttt aaaattataa aatatctaat tacagtttag gaggaatagg
     6961 ttatgaaata tgaagaatat gaggtagctt taaaaagaat tgaagagttt gaagaaataa
     7021 cagaatacaa acaaattgct gacgaagtat tagaaaaatt agattctaat aattgtatat
     7081 taaaaataaa atttgatttt ggcgattgtc taacatacga cttaaattta agttctgatg
     7141 atgaacaaga gaattttata aaaaaagaga tgtattgtgc atttagaaat ataaaaaata
     7201 aacttgaaga aatgatggaa gaaatataag taaaaatatc taattaaaac agtttagaaa
     7261 attgcaaagt gtcttttgat gtaaaattgt tgaagtgttt tgtaactttc taaaatggaa
     7321 gtgagaggag aataatataa tggatttaag atgtataagt ctacactttt attacaagat
     7381 agttgattta gaaaaatttg aggatgtgtt ttattctcaa acaaatttta aagaagttac
     7441 tggagacatt gataaaataa catcattaga aacttttaaa tattatagtg attactatag
     7501 gaaaattata gcaaaaggtt tggagattga tgaaaacaag gtaattagtg taagttgtga
     7561 ggaatactat gaaaatactt atgaagatta gaggattgta gctttataat tgctgtgatg
     7621 taaggagggt attaaatatg gaaaatatag aattaacacc tagaaattat tttgaggata
     7681 taaaaaatag aaagcaaaag aatacaaatg aaaatcttga tatagtatat gaaaattctt
     7741 tgattctttt aaacaaatat attataactg gtcaaaaaaa gggtgctaag aaattaatgt
     7801 ttttacttga atgtattgaa aaagaaagaa atgtaataga gctgggaata gatacatttg
     7861 tttataaaga agatatagaa aaatatattg atgatatatc taaaaataca gtaaaaataa
     7921 tagaacttga aaattatgaa agagaaatac cagatgaaat agtagaaata atagagaaaa
     7981 caaaagatat atttgataaa atgtatgttt tgtttactga ttatacaggt aaaattacaa
     8041 aggaaataag caaagagaga agagataaag acccaatact atttggtgtt ttttcagatg
     8101 agaaaagcag agctattgta gatagattct attatcttgg agattgggaa gatgagtatt
     8161 gtgatttaac attagagaaa atgataaatg attataagaa aaaaggaaga caagacatat
     8221 taaaacatat aattacacct aaatcattag atgagttaaa aaaagaatta gattctttag
     8281 aaaaaaataa tgatacatat aagcaacttg ccaatataaa gccattatct actacaaaaa
     8341 actctagtgt agaaccacct aaattaaata tatttaaaaa aataaaaaat agagtattta
     8401 aaaatgaaaa gtaatgttga tttaacagag aatagggatt ttagagctgt aagaggaaga
     8461 ggcatcaata ttttaggtat cgaagatgca ccaaatagaa tgttgaatgg ttttagtgat
     8521 tttggaattg gacgtcttta tggtaaaaat ttagaatttg aatttaataa aaatgaagta
     8581 tttttaactg aaaatgcgaa tgatagagag gaaaaaaagt ttcttatact ttgtgaagaa
     8641 aataaggtgt gtgtcagatg tggagatgaa ttaaaagagg gatgggataa agtaagcata
     8701 gaactatgta ataaatgtta tgaaatgtta gatgttgagt ataaaaataa caagataatt
     8761 agagaattat tttttagaga tagagatgta aatatagagt tactttaaga ggtatataag
     8821 aaaaataagt agaggaggaa tttatatgaa agatgtttgg ttatatagtt gggatgatga
     8881 atgttttaga agtgatgaat acgaaagtaa agaagaagct gtcgaggcag ctaaggaaga
     8941 acttaagagg ctcggagaaa ttaaacgatg ggcttatatt ggaaaaaaag aagaagctga
     9001 tattcccgat atagatgcaa aagatgcttt agaatgtatt caagatagaa ttgatgacga
     9061 atacggagag tatggagaag attggtttga aaatatacgt gctgaagaca tattgatact
     9121 agagagtaga ataagcgaag tatttaaaaa atggatagat gactttaaat ataaaccata
     9181 ttggtttgct gttagagata tagaagaaat agaactaaaa aatgctgtca attaaaacta
     9241 gtattttcaa tgatgacaac agtatgacaa gaataatgag tatataaagg agtgatgttt
     9301 attaaaggtt ttagatgtat atgcagacat aagttttcaa ggataaaaga ttgttatttg
     9361 attacaacaa aaagtcagca gtatacttgt aagatttggg tgtgtaaata ttgtggacaa
     9421 attgtagctg gttcaactcc tgtttatcat cctaatatga aagtcaatga gtattgtgta
     9481 aagtgtgagc ttttagcatg tgcaaataat tatgaaggat attgtaattc aaaagataaa
     9541 gaaattgagt ttaagtctgt ttgtttagat gatggagaac agttcttaag atgtgaaaat
     9601 tttgattggg aataaaatag gagggtttta aaatatgaaa ataaatgcat atgttaaaac
     9661 taataaaatt ggttcaggtt gtgagtttga gtttgaaata gatgatgata atttaaaaaa
     9721 tatgtctgat gaagagaaaa aggattatat agataaaata gcatgggatt acgtttttga
     9781 aagttttata gattggaatt ggtacgaggt cgaagattaa gttaaaacaa taattttaat
     9841 tatttataaa aggaggattt aatgtgaata aagataattt agaaacaatt atagaaggtt
     9901 ataaggtatt taatccagat tggacttgca gaaattttaa atatgaagta ggtaagactt
     9961 atgaacatga tggagatata gaagtatgtg aagctggatt tcatttctgt caaaaagcaa
    10021 ttaactgttt taactattat tcttttgata ataaaaataa agttgctaaa attgaagcaa
    10081 taggattaat taagacagat ggtgataaat ctgtcacaaa taaaattaca atagtgaaag
    10141 aaatcaaatg ggaagaatta ttaaatattg taaatatagg gaataacaat ataggatatg
    10201 gtaatacagg agattataat acaggaagtt acaatacagg agattataat ataggaagtt
    10261 acaatacagg agattataat atagaaaatt gtaatacagg atatcgtaat gaaggatgtt
    10321 gtaatgaagg atgttggaat acaggaaatt ataatatagg gaattgtaac tcaggagatt
    10381 ggaacatagg agattataat tcaggggtat tttgtacaga tgttccaaaa atcagaatgt
    10441 ttaataaaga aactgattta acatatgagg attggataaa tagtgaggca cgaaatattt
    10501 taaaaagaaa tagttattta acaaaatgga ttcacattga tgacatgaca gaagaagaaa
    10561 aagaaaataa tccaggatat gaaattaata atggttattt aaaggtgttt acatttgagg
    10621 agatgtgcaa gaacatgtgg aataacttaa ctgatgaaga aaagaaaatt attatagaca
    10681 tacctaattt tgatgcggat atttttaaag agataactgg aatagaagta attaaattaa
    10741 aaataaagga atgcaacata gtggacaaac aagaatatac aaattgttgt ggtaattgta
    10801 aagaatgtga tgaatatcat aagatgttat gtaattcatt atatgaggag gaataagaat
    10861 ggctaaatat gatttattgc taaaattaaa taatatttta ttcaagatgg aaaaacattg
    10921 cgatgaaatg attaaaaata ataacgataa agaattgaga acctgtacta attgcgaatt
    10981 tgcttattat ggtagttatg ttgaaagtgg atgtgtttgt acagatattg aaggatttgg
    11041 catacatgat atggtaaaaa gaattattaa aaaaaataaa agaaagaaaa acaaaaagat
    11101 ggaatataga aaaaattact aataggataa atgaccatat gaatattcag gaggatatag
    11161 aaaaatggga taaatttgaa acaccaatta ttagtaaaga ggtagatttt tatttattga
    11221 aaataggatt tcaatatgga aggaatgatg ggagaaatac cttagtagga attaaggata
    11281 tgcgtcatat atcgataagt aaagttaatt ctaacttatt atcaatagat atacataaat
    11341 ttattaaaca agaaaatgga aaacttagtg aatttaaagc acatgaatat atagaaaagt
    11401 aattaaaaca aatgttttaa tgtgatttta cctagtaggt acaaggaggt ttttaatgaa
    11461 agagctttta gaagtaaaaa aaatatttga tagtttagca tctacatcta gtagaaaaga
    11521 aaaagagaga atattagaaa aatataaaaa taatagaatg tttgttgaat gtttacaatt
    11581 tctgctagat tcaaatattc tcactggaat atctaagaat aaaatatgta agaatctaaa
    11641 taatactagt cataatgaat tagagaacat atatgatatg cttgactatt taattaagaa
    11701 taatactggc agaaatatag atgtaaaaac aatacaagta tttgccagta aagatgaaaa
    11761 attaaaagat tttattttta acttagctac aaaatcaatt aaattaggaa taacatataa
    11821 gactgtagac aaaataatgc caggtttaat tatctaataa attttgtgtt ttttacaaaa
    11881 aactattgag aacatagagg ttatatgata taataaaaat atattgagaa cagagaagtt
    11941 aaaaaggggg gcaatgagat gactgacaaa ataataatag caagtgtaga tggagaggaa
    12001 atgatattta gaactgaaat gaaaaagtta gaaaaaggag atagtcttat agtttctgta
    12061 aatggtgtaa aaagattagc tctattcatt aggtatataa gtacttgtga agaatatata
    12121 gaagatatag catttcaaaa agtagatgat atattatatg aaaacacaat tgccaaagaa
    12181 aaagaaagag ttaaaaaggt atttaaagat aagtgtatga aaaattataa acctattgtt
    12241 aatacagaaa tagactctta tttgagggaa tgtggtttca attgtgatgg tagacaattt
    12301 ttcaaaagta tagacaatac acaaatttta attagcaggg attgtgataa agatgttttg
    12361 actgtatcga aaggtttgac aataactaaa acagatataa caaataaaaa tttaattaca
    12421 gttattaaaa taataaataa tagtctatta atagttaggt aatagaaata aatattgaac
    12481 ttttaataag aaaaaagatt ttaaataaaa ggagtgattt aatgaagaat gttgttaaaa
    12541 gagatgggag gatagttccc tttgatgaaa agaaaataat aaatgcaata gaaaaaagct
    12601 ttttatcatt aaggaacaaa ggaaaaagtg aagtatttga tagagaatta gcaacactaa
    12661 tagctaaaaa aataaaaaat aaaccatgtg aaaaaatgca tatagaagat atacaagata
    12721 taatcgaatt tagcttaatg gctagtaaaa agaaagatgt cgctaaagaa tatataacat
    12781 acagagagaa aagaaatata gcaagaggaa gaaaaactta taatgattat atgtctattg
    12841 taaatactga aagtaatgat ataactaaag aaaatgcaaa tatgaacgca gatactccag
    12901 caggtatgat gatgaagttt gcaagtgaaa ctactaaaac ttttactgac catgtacttt
    12961 tatctgaaga agcaaaagaa gcaatgatgg ataattatat acatatacat gataaagact
    13021 attatcctac gaaatcattg acttgtatcc aacatccttt agataaaatt tttaataatg
    13081 gttttagagc gggacatggc tctagtagac ctactaaaag aatagagact tcaagtatat
    13141 taggatgtat tagtttagag actgtacaaa atgaaatgca tggaggacag gcgataccag
    13201 cttttgattt ttataatgct cagagtgtaa gaaaaacatt tatagaagaa ataaaaaata
    13261 tagaaataaa ggatgatatt gatttatctc atttatacaa tatagaaata gaggattata
    13321 taattaagga cttaaaagaa ttagacagcg aaaaaagaaa tgttcaacat gctataaaca
    13381 gaactgtatg gagagttcat caatcaatgg agtcatttat acataatatg aacacaattc
    13441 attcaagggg tggaaatcag gtcgtgttta gttctataaa ttatggtaca gatacttcag
    13501 cagaaggtag atgtataata agagaaatac taaaatctac ttatgaagga gtcggaaatg
    13561 gagaaacacc aattttccca attcaaatct ggaaagtgaa agaaggtgtg aactataatc
    13621 ctactgatag aaattatgac ttatatgaat tagcatgtaa agtatcagct aaaagatttt
    13681 tccctaattt tttaaattta gatgcaacct tcaataagca tgtaaaatgg aaaatgtgcg
    13741 acccaaacag atatatttat gaagtagcaa caatggggtg tcgtacaagg gtatttgaag
    13801 atttgcatgg agaaaaaaca agtataggaa gaggaaatct atcttttacg actcttaatt
    13861 tagtaaaaat ggcattagag gctaaagaaa aatatccaaa taatgaagct aatagagttg
    13921 cagagtttat ttttcttctt gataaatatt cagatgtagt agcaagacaa ttatatgata
    13981 gatacttatt tcaatgtact gctaaagcaa aacagtttcc actattaatg agtgggttat
    14041 ggaaaaactc agaaaattta aatccaaatg atgatgtaag cgaagtttta aagcatggaa
    14101 cacttggggt aggatttata ggtctagcag aatgtttaat agctcttata ggtgaacatc
    14161 atggtgaaag cgaaaaggca caaaaattag gtttagaaat catatcactt ctaaagtcta
    14221 aagtagaaaa atataaaaag aaatataacc tcaattattc tgtattaggc acacccgcag
    14281 aaggtctttc tggaaaattt actcaaaaag ataaaaaaat atttggagaa ataaaaaata
    14341 ttacagacaa agattattat actaatagtt ctcatgttcc agtttggtat aactgcaatg
    14401 ttgaacataa agcgaaaata gaagcacctt accatgaatt agaaggtggt ggccacatat
    14461 tttatgttga attagatggg gatgcaacac ataatcctaa agctataatg aaagttgtgg
    14521 atttaatgaa aaagtacaat atgggatatg gttctgtaaa tcatacaaga agtagatgta
    14581 tgaattgtgg ttttgaaaac gcagattcag agttaaaaga atgtcctaaa tgtggcagca
    14641 atagtataaa tattatccaa agaattacag gttatttagt tggaactacg gatagctgga
    14701 ataatgcaaa attagctgag ttaaaggata gagtcacaca tggataaaga ctatatttat
    14761 attttagata taaaacatga cacaattgtt gatggagaag ggtttagaac atctatttat
    14821 tgttctggat gtaatcatat gtgtagaggc tgccacaatc ctcaatcttg ggatataaaa
    14881 aatggaactt taactaaagt attagatatt tataaggaaa tcatttcaaa taaattttca
    14941 gatgtaacat tttcaggagg agacccactt ctacaactgc aaggatttgt tcatttagct
    15001 aaattaatta aaaagaatac aaacaaaaca atatggtgtt atacaggttt taagtttgag
    15061 gaactcataa cagacaatga aaagctagaa ttgttaaaat taatagatgt tcttgtggat
    15121 ggaaaatttg aagaagataa aaaatcttta gagttgattt ttaaaggttc gtcaaatcaa
    15181 aggataattg atgttcaaaa aacattatat gaaaaacaaa tagtattata tgaataagag
    15241 aggcttagcc ctctctttgg aggtaagtgt tatgaccaaa gaagaattta ttactaaatt
    15301 tcttaaagaa cagcagagaa gaataaaggt tttatattat gaagttgaaa atttcaagat
    15361 ggatagaagg tttaatgaga ggaaaaagtc taggagacat taaaatgttt cttttaatat
    15421 aaggaggaag tagctatatg tattttgaaa cggacattat aaaaaataag aatactgtaa
    15481 ataaaataat aaaatattgt ggtggaaata ctgaaaaatt gaaagattgg ctaaaagaaa
    15541 tagaaaataa aagtatcagt agtttagaag aattaaaaga atatccatta aaaagaaatt
    15601 ataaatttgt attttccaaa gaagaagctc ctaatttaga taagcttata aaaatgaaac
    15661 taagagataa ttgtttagaa aaatgtgaga tttgtagttt agaaaatctc ttaagtatgc
    15721 taaatgatga gattttagat ttaaataaag aagatatctc ttatgaagat aatattgaat
    15781 ggattgaaaa ttgtaaagaa gaactaatta aattaaaggt aggaagtatt tgcatagatt
    15841 ggtagaaaaa taaaggagtg atattgtgag attttggtgt gagaattgta ataaatactt
    15901 caatgcggaa gaaacattac aggaatataa ttatttttta aatgaagaga tacttatatg
    15961 ccctagttgc aaaaaagatt taatacctat agctagggaa acagaattat cattaggttt
    16021 tgatagtgat acaaaccaat taacttatgt tgaatatgat tctagtgatt atagtttact
    16081 tagaaaggta aatgcagatg ttgaagatgt agtaaaacct ataatacatt acataagaaa
    16141 tttaaataaa aatagtttag atttaaatgg aattacaata acaatgaatg gtaatagaga
    16201 aggaaaaaga cttgatggat taaattatga agaaggagtg gttatggata atttgataaa
    16261 tgcatggaat ggattttgca aattaaaaag acagcatcct tctgagttag gagattttca
    16321 aaatgctata catcaagctc aacaagtatt agggttaaga gttttaagaa atgattatcc
    16381 aaaaggttgg attaaaaaat aaatataata aggagagaaa ccatgtctac aaaaactata
    16441 ttaaaaaata tagatataaa agatgataaa gcagcagaaa gactaatatc agctttggaa
    16501 gagtcggaaa ataaacaaga ttcagattta gatattaaaa agatatttga attaatagac
    16561 aattctattt cctcttatat tatatatgga actccaaaag cattaagtaa atttgaaagt
    16621 atcttaaata tctttaaaat agacaaagca aattctcaac atgtccaaaa atttataaaa
    16681 gtaccagcaa tagatttagg taaatactgt gattataaaa ataataaagc cctttttgat
    16741 gataatacag tgtacattat tccaaaagat acaaaattaa aaatatataa agacgaatat
    16801 aagtatgttg ttttgaaata gatgatgata tatataaaaa ggaggtttta atgaccaaat
    16861 ataaaataga agatagaact aatcttataa ttcatcaatc taaaaaacat aatattacct
    16921 ttactttaat tctagaaaat gaagataaaa gagaaatgct taaaaaagtt ttaagatgct
    16981 taaagaagga acgtattgtg tttctattga aggtaaatta agcagtgagg aagtagaatt
    17041 aggaaatgtt acaataacaa tgtttgtaga tggaaatatt attttaaaaa tggatgtgtt
    17101 gaaatatctg gtgagtataa agttgaatag ataaataaaa ttagagggga gaatgaatat
    17161 ggaatttaat aattttaaaa aacaagttca agagagattt aataaaatga caactggaat
    17221 ctcttatata tatgaagtag atttagataa agataggctg tggaatttat atttagatag
    17281 ttttccagaa ggtacaaacg aaatatttag agagagaaga gaatatgatt gctcttgttg
    17341 taggcaattt attaaaaata taggaaatgt agtaactata aaggataata aaatatcatc
    17401 aatctgggac atagacacag attgtcctac atttaaacct gttgtaaaag cactatctga
    17461 atatgttaaa tctcataata taaatgatat atatgtaagt aaatttaaga aaataggaac
    17521 tgataaaaat tttgaaaaca catctgaaaa agtatatgaa tggaatcatt tatatataga
    17581 attacctgat agatttgtgg atgaaactaa taaatctgta ggagatatta aaggaagaat
    17641 tagagatgtt aagaatgtat ttaaacgttc attggatgaa ataagcgaag atagtgtatc
    17701 tatagtgcta gacttaataa atcaaaagtc tctctataag ggggaggaat ggcaaaaccc
    17761 attgatagaa tttctaaaat ataaaagaaa ttatattaaa ttacaaactg aaactgataa
    17821 gcaaaatttc gcatgggaac aatctgtaaa agctggagtt gttataggta aaataagaaa
    17881 tcatagtatt ggtactttat tacttgatat atcagaagga gttgaattag ataaagctgt
    17941 aagtagatat gaaaaaatag tagctccatc taactataaa agaccaaagg cgatatatac
    18001 taaaaaaatg ttagacgaag caaagaaaac tataactgaa ctaggatata tggattcact
    18061 tcaaagaaga cacgctacaa tagatgatat tactgtaaat aacatattat ttgttaataa
    18121 ggattcagca aaacaaatgg gagaatttga tatatttgat gaaatgtcta aaggcatagg
    18181 tatagattct aagaagtttt caaaagtaga agaattaaat atagaagagt ttattcaaaa
    18241 tgttctaccc acatcaaaag aagttgaact gttattagaa aatagacata tagacaattt
    18301 tgtatcatta atagcaccag ttaataagga ggccaaatca atgttgaaat gggataataa
    18361 ctttggatgg gcttacgctg gaaatatgac agatagtagc atgaaggaga atgttaaaaa
    18421 tgcaggtgga aaagtagatg gcgtattaag atttagtatt caatggaatg ataccgaatt
    18481 cgataacaac gatttagatg ctcattgtat agaaccaaat ggaaatcata tatattttgg
    18541 taataaaacg aattactcta caactggtat gttagatata gatataatgc atccaaaaag
    18601 aggtaatgtt gcagttgaaa atataacttg gtctgatata aacaaaatga gattaggtac
    18661 atataagttt tatgtaaata attactcgaa gagaagtgga atatctggat ttagagctga
    18721 aatagagttt aatggtgaaa tttattcatt tgattataac aaacctttaa gagatggaga
    18781 aaatgtaatg gttgctgaag taacgttaga tataggtaat agatttaaaa ttaagccatt
    18841 gttaccttct aatgtttcaa ataaagaaat atggggatta actacaaatc aatttatacc
    18901 tgtaagtgta ataatgaact ctcctaatta ttgggatggg caacaaggaa taggaaataa
    18961 acattatttc tttatgttga aagattgtat aaatgaagaa acagtaaatg gtttttataa
    19021 tgaatatcta aaaaatgatt taaatgtaca tagaaaagtt tttgaagcac taggaagtaa
    19081 aatgtatgtt ccttttagtg aaaatcaaat gagtggatta gggtttagct ctaccagaag
    19141 aaatgaagta attgtaaaag ttagaggaag ttatgaaaga atattaaaaa taaaattcta
    19201 aagagagaga gaggtaaata atagtatgga aaatgtattt gagatggcaa caagaaataa
    19261 atttagattt gaatttagag gattaatttc agttgaggat ttatgggatt taaaattgga
    19321 gcagttagac gaagtatata aaaccttaac aacagatatg aaaaaagcta atgaggagag
    19381 cttattaaag ataaaaacaa aagaccaaaa ggaagtagaa ttaaaaataa aaatagttaa
    19441 atatatagta gagttaagac ttgcagaaat agaaaataga aataaagaaa agaagaaaaa
    19501 agaacaaaag caacatatat tagagctaat aaataagaag gaaaatcaag aattggaaaa
    19561 taaatcaata gaagaattaa aatcaatgat taatagttta gagtagttct agtaaaagat
    19621 gctcttgttt aatagagcat ctttgaataa aaaacagatt ctaataagaa agggtattag
    19681 aatgtggata gtaaaataga agttggaagc atatggggat tagaagatgg gatagcacaa
    19741 cctcaatatg taggaacaaa agttaaagtg gtttctattt catatataga agaatttaat
    19801 aaaagactta taacgtttat ggaaattgaa gaaaaagaag aagatacttg gatagatgca
    19861 gattgggaag atgttttttt aaatgttttc tttaaattag actaattaaa acaacagttt
    19921 taaatgaata gaaattttaa aattgaggag ggggtaatat aattagtttt tttaagaaat
    19981 caataaagtc agaatataca gagagtgaaa cttataaaaa taaaaaaccc atagaaagat
    20041 tttttaaata tgaaataaaa actaaggaga atatattaca tattggttta gaattattta
    20101 tttatgaaga aggaattagg gggttgtttt taaaaaaaaa tgtgtttttg tataacaaaa
    20161 tatttaagga aattattatt gatgatattt atagagtaaa ccttgcagaa ggtaggttta
    20221 tcagaatgtg cgaccattta gaggaatata ttatgatttt aataaaagat aaaatagaag
    20281 aacaatattt taaaaatgat aagtttaaag aaataagaca agaaataata agatttgaaa
    20341 aaaagggtca ggttactata aagcttaaag atgaataaaa ttaaaaagga gaagtaataa
    20401 atatggatga caaagtatta atacagttaa caaatggagc aattattaag gagaaagata
    20461 ataagtgtat tctaaaggcg atgtagtgaa agtgtggtgc tatcagtgtg aaaaaataga
    20521 atatcaagaa tttgtaggat attttaattc ttattttgat aattataaat taaagaaacg
    20581 atggaaatgc tctaagtgtg gtgttatcaa taatttttaa ggatttaatt aatggaggaa
    20641 tgagttatga aaataaagaa aataatttta ggattagata acttagagaa tgtagagtta
    20701 ggtattggag atgtagggga gttttattta gataatatac atgaaattat tggaggattt
    20761 gctttgagtt atataggtaa atgcaaaaag gcagattatt tacatataca aatacataaa
    20821 cgtgttgatg aaattcaaag attgcatgat tataaggata ttataaatgt aaagatagaa
    20881 tacgaagacg gaagtgtgga ggatatatat gtaccttgga agcaagaagg ggatttcttg
    20941 ggtgtagata atctttatca aacttctttt attagtaaac aaggttattt acatatagtt
    21001 atctctaagg ataaaacagt agaagaagtc ttcaatgaag aatttgagga tgatgaaagg
    21061 gtagaattta gttttgaaat gtatggaata taaaatttaa taggcaaggt gatacatatg
    21121 ggattagatt taggttttta ttcagtaaaa acaaaagtta cagatttaga cacttattta
    21181 aataaaacaa gagttcaaaa aagtaaagag tgtaaaactg tagaagattt ttatgagttt
    21241 gaagaactat tagatattga tatagcttta gaaaattggt caattattcg tatgatatat
    21301 aaagccaaat gggaaaaagg ttatgaaagt tatgaagatg aagccatact tattacaaag
    21361 gaagatttgg aagattatat aattcctttt cttaaacaaa aagatataaa ttctaaaata
    21421 tacaataagg ataaagagta ttattataaa aatgataaaa aagtaacagt tgatgatagg
    21481 tatacacagg agttttggga tttagtatta aaagaatttc aaaaaatgtt aaatataatt
    21541 gattttgaaa aacatagttt gattattatg tattggtatt aaaatacagg ttttaaaagg
    21601 agaataataa atggaagttc aagggaaata taacaaagct aaaatattta cagagaatgt
    21661 agattcgata acaataggac aaataataaa tttatgtaat caaaaggaat ttaaagaatc
    21721 cagtataaga attatgcctg attgtcatgc tggtaaagga tgtacaatag gaacaacaat
    21781 gactataagt gataaagtag taccaaatct ggtaggggtg gatataggtt gtggaatggc
    21841 tactattcca ttagaaaaat atattaataa tataaattat acaaaattag ataagattat
    21901 aagaaatgat attcctcatg gatttaaaat acataataag tcaagagaaa aactattaaa
    21961 gaatttattt aatatagaca taaataatct tagatgcgag gtgaataaaa atagagcata
    22021 tatgtcgtta ggaactttag gtggaggcaa tcactttata gaagttgata aaagtgaaaa
    22081 aggtcagatg tatcttactg tttatacagg ttcaagaaat ttaggaaaac aaatagctga
    22141 ttattaccaa gacaaagcta taaaatattg tgttgaaaaa tataaaaagt catatgatga
    22201 tgcaaaagag tttttaatat ctactctaaa aaaagacaat aaagaacatt tgataaatga
    22261 atatttgaaa gaccttagtg atagaaaagt gaataagcca catgatgatt tatgttattt
    22321 agaaaatgat ttaatggaag attatttaca tgatatgatt atagctcaaa aatacgctgc
    22381 tgcaaataga atagttataa ttgcggatat attgtttaaa tataacagcc cagatgtaaa
    22441 ttatgaccat tttataagag atattaaatg tgatgttata gaatgtattc ataactatat
    22501 agatatggat agtaaaatac ttagaaaagg agcaatttct gcaaataaag atgaaacagt
    22561 aataattcca ataaacatga gagatgggat tatattagga aaaggtaaag gtaattctga
    22621 gtggaattat tcagcaccac atggagcagg aagaatttta tcaagaggaa aagctaaaga
    22681 aaaaatatct ttagatgaat ttgaagaatc tatgaaagaa atatttacaa cttgcgtagg
    22741 tcaatcaaca ctagatgaag caccacaagc atacaaacct atagaggaca tcttaaacaa
    22801 tataggagat actattgaga taatggaggt gttaaagcca atttataatt ttaaaagcaa
    22861 ttagaatctt gttatttaaa taaaatcaaa aggataaaaa aaagatatgc ctaattaatt
    22921 tatgttgaat ttatagataa tgaagaaaat gataattgcg atatagttag agtctaatat
    22981 gaagacattg agaatatagt agaacctata gtttcttata tgaagagttt aaatacatgt
    23041 aaaatcgatt taaatgatat tacaataata aagaaatgag gacaatatga aagagtatat
    23101 atattgagtg aaaataagtg aaataaaaga aatatatggt ggaggtggga attgtatatg
    23161 acaaaaaata ttaaaatata atttaattat ataaatgatt agaattaaga ttttgttata
    23221 taggagtaat tatgagatta gatgatagtc agataggttt aataatgtat atgataggat
    23281 ttgttcatta tgacaaagag caatttcaat tttatatgga tacaatatat ggaaaggaat
    23341 attcagaaaa agaaatagtc aatatggtag aacatctaag acaagatatt aagattcaaa
    23401 gatttaaata aaattaagaa ggagtgatgt gtatgagttt tagatgtgga gggattgctt
    23461 atgcagatgg tatagatgta ggcgtaggat tttgctatga accactacat tgtgattgtg
    23521 gttggtcaga aatgtgtgct tatgaagata aagaagaatt tgatgaatgg gaatataaaa
    23581 aataattaag gagatgatta aatgggacat tatagtgaag aatgggaaga atgtgcattt
    23641 tgtaaaacaa atagtaataa atgtgatgta tatgataagt tatttgtttc tactccaata
    23701 tgtgaaaaat gtgctactga gataactaaa ataattgaag aaaataaaag aagagataca
    23761 atagatatac gaaacatgca tattgaatct ataaataact tatttcatga ttgggatata
    23821 aactctaaat taacatataa ggatatgtca actataattg aattattaaa tgaagtaaaa
    23881 aatagagata attatgaaaa tataactaca gttgatgaat attatggatt aaacattaaa
    23941 tcatatagaa agagacgttt ttaaaagagg aaaaatataa tattgtaaat gattaatcta
    24001 aatttcaatt ctaagaaatt catttaagca attcaaaaac tctaaatata tattcttatt
    24061 tacaattcaa ttaaaaataa aggagtgaaa ttatattgaa aagattttta ttaatagggg
    24121 tattaggaac aatgttaata ggaatgtctg gatgtacagg gatacaaagc tcaatgaagg
    24181 atgccgaaag tgatgtatct ggtcttaata gagttgtaaa tgtgtattca gacaatggtg
    24241 atgtattgaa aacatataca agtaaaaata tgagagtcaa agatggagat ggtggtacta
    24301 taacattaga ttttgatggt aagagggtaa ttatatgcaa tgctcatgta gtaatagaag
    24361 aaaatcaatc taacaaataa aggagaatca aatgtttaaa gtcttaaaaa caattcttat
    24421 aataatttgg gtagtagata ttttaaactt cccacaattt caattcttag atacaacata
    24481 cccaattaat accttagcct ggttgctaat ctggattctt ataccatcaa gtagtatata
    24541 tatcaataaa gaagaataaa aggagggagt attagtttga ttttaaatat ttgtgcaatt
    24601 tttaatttat ggtgtatgtt agctaattat ttatggggaa gtactagtgg aatgatactt
    24661 aatggatttt gtcttatagc attactgtta ttgaataata acttagatta aaagtattat
    24721 tttaaggaga ttaatgtgtg ataggtaaat taaattttat tgaaatatca aaagatatat
    24781 ctgatgatga gcttaatgat ttgttggtaa aacacccaga tgcagtcatg tttgtagatg
    24841 aagaaatgat aactagatgg aataaactaa aaaataaaga caaataaaaa aaggggagat
    24901 gtttataagt agtacaaata gaagtaatgt aagagatttt catataagtg attactatag
    24961 aactccagtt ataaaaatta aagagttttt aaatgaattt agtaaatatg aagatatatt
    25021 caattcaaag ataaaaatat tagacccatg tgcaggtgga gacaatacga atccaatgag
    25081 ctacccaact gcaattcaag aattttcaaa tcaagatatt tcaatagata cagtagatat
    25141 aagagatgat tcaagggcaa atataaagca agattattta aagtttcagc ccaaagaaaa
    25201 atacgatgta atcattacta accctccatt taatttaagt ttagatataa ttaataaagc
    25261 tttaaatgat gtaagagaag gtggtttcgt tattatgctg ctaatattga attatctagg
    25321 aggaaaaata aggcaggagc tatggaaaaa taatatgcct aagtatattt ttgtacacaa
    25381 taaaagaata agctttacag atgatggaaa gacagacagt attgaatatg ctcattttgt
    25441 atggcagaag ggatataatc caaatttttc tcagttaaaa gtattaataa gtcaaaaaaa
    25501 ttgaggtgca aaatgaaaat taaatatgta gtaattgaag gatatattac atcaaaaaat
    25561 gatggtttta aatgattata aaatgtcagt tttaaagggg gctaatcata tatggatatt
    25621 aagatagata tgtgtttatt agagatggaa ataaatagaa gaagatgtga gtatgctgcc
    25681 agaacaggaa gatatcctaa ttctcttatt ttgtgggaag gttactatag ccttcttaat
    25741 atccaatata tagaggataa aacaagagga tatattacta aatttaatga catggatgtt
    25801 ataaaatgta atgacttttt aaaaatagaa ttatttgaaa agaatattaa taattaaaat
    25861 ttgtttttta agttgaggtg atgagttgag atataataat tatcatagtc ataaaattta
    25921 ttcaaatata aggtcgttag atgtaattac taaaccacaa caatatatag atagagcaat
    25981 aaaactaggt caaaatacat attttacgac agaacatgga tatcaaggaa atgtatatga
    26041 ggcaaaaact ttatgtgatg aatataaatt aaaaatgatt attggagcag aattttacta
    26101 tgtaaatgat ataaatgaga aagatagagg aaattatcat ttaatagtaa tcgctaaaaa
    26161 taatgatggt tacaagcaaa taaataaagc tttaagttta gctaataaaa atggatatta
    26221 ctataaacca agaatagatg aaaaaatatt atttgagata tttaaaccag gggatgtagt
    26281 tattactact gcatgtgtag ctggaatatt aaaacttgag aatagagaag aattaatact
    26341 aaaactaaag aattatttta aaaataattt ctttcttgaa gtacaatctc atcctcataa
    26401 aactcaagct ttgcacaata aggatgtttt agaattaagc aacaaatata atatagatat
    26461 aatacacgcc aatgacagtc attatattta tccagaagaa tcaaaatata gaactaaatt
    26521 tcttaaagct aaaggtataa attatcctga agaagacggt tttattttag attatccaaa
    26581 ttcagaggat atatttaaaa gatatgaaaa acaaaatgta ttaacaaagc aagaagttga
    26641 aagagcatta aaaaatactc taatatttga tgagtgtgaa gaaatcacct taataaatga
    26701 tgatataaaa ttaccatcaa tatctaaaaa tccaaataaa gaactgaaag aaatattaaa
    26761 taaagaatgg ttagagaaaa gaaaaaatat ccctaaaaat agatggaatg aatacttaga
    26821 tgctataagg tatgaatttg atattataga gaaaactcat atggaggatt actttattat
    26881 agattataaa atagttcaaa gagcaaaaca agaatataat ggtcttctta ctaaaacagg
    26941 gagaggttca gcaccttcat ttataataac taatctttta gggcttactg aaatagatag
    27001 attaaatgct ccggtaccat tattccctac tagatttatg agtgttgaaa gaatactatc
    27061 agcaaaatct ttgccagata tagatttaaa tgctgaagat gctgaaccat ttatacaagc
    27121 aactagagat ttattaggag aagaaaattg tgcatggatg ataagttata agccattaca
    27181 agatgcttca gcttttaggc tatggtgtaa agcaaatgat atgaaactat ctgagtatga
    27241 tgaagtagct aaaaacttag ataaatacac aaatgatgtt ttttggaaag atgtaataaa
    27301 ggaatcaaag gtatttgtag gagtagtaga aagtgtttct ttctcaccat gctctatgct
    27361 tatatatgat agaccaattg atgaagaagt aggattatta aaaactaagg atggggtatg
    27421 ttgtaatata gatggatatt attgtgataa gtataaatat ttaaaaaatg attatttaac
    27481 agtgaaagta tggtcattaa taagaaaaac ttgtgaatta gcaaatatta gtattcctac
    27541 aattgaagaa ctaaacatct tattagatgc taaaacctat gacatatata aggacaaatt
    27601 aacttgcact ataaatcagg tagattcaga ttatgctaca aacctggcat ctaaatacaa
    27661 aatatctagt gtggctgaaa caagtgcatt tgtagctagt atcagaccag gatttgcatc
    27721 tttattagac aattttatta acagagagtc atatacaact aacgttaaag aactagatat
    27781 attattagaa gatagttatc attatcttat gtatcaagaa tcaataatga aatatttaat
    27841 atggttagga atagaggaat ctgagagtta tgatattata aataagatta aaaaaaagag
    27901 atttaaggaa aaagagttga aagaattgca tatcaaacta aaaaataact ggattaaaaa
    27961 agtcggaact gaaaataatt ttgaagatac atggcaagtt gtaaacgatg ctgcctcata
    28021 ttcctttaat gcttcacata gtctttcata tgcatatgac agcttatact gtgcatacct
    28081 taagtcacat tatccattgg aatattatac tgtagcattc aatttatata atgaagatac
    28141 agagagaaca agaaaattaa cagatgaaat agagtatttt agtattaaat tagaaaatcc
    28201 taaatttaga ttttcaaaat ctgaatactt ctttaataga gacacaaata gtatttacaa
    28261 gggcattgaa tctattaaat ttttaaattc tgatatagga gagtatctat attctcttaa
    28321 agataataaa tatgattcat ttttagagct attaatagat ttgcaggggc atataaactc
    28381 taaacaatta tctatactga ttaagctaga tttctttgag gaatttggaa agtccaacaa
    28441 acttttagag acttatgata tctataactc aatctatagt aaaaaacaat ttaaaagaga
    28501 taatttacca tgcaatataa ataccatgag aaaatactct aataaagaaa cagaaaaaat
    28561 atttaaagaa gttgatacaa aagaattatg ttcttatctt gaaagtcaaa ttgaaaatat
    28621 tgatatacca atcagtgaaa aaatacaagc tcattttgaa tttgtaggct catgcaatat
    28681 aaaagacgac aattcaaatc caaggtattg tttagtaata gatatagata ctaaatattc
    28741 tcctaaaatt acactataca atctaaaatc aggtaataca aagattttta aggtaagtaa
    28801 atctattttt gaggataata aaattgatat ttatgactta gtttatttaa aatcaactaa
    28861 agagaaagct aaaagtaaaa aagtagatga aaaatggatt aagagtaata cagaaacaga
    28921 atggtgggta caagaatatt ggaaagtaga aaattaaaaa tatgacccca tatatgacat
    28981 tctaaggcca tgtataaatg atagcttgtg tttatacctt gcagtcttaa aatggcgtat
    29041 acaaggtcgg tgaaaggtag tgatttcaat acttatagac gaacaaagac atcgtgattt
    29101 actagagaaa aacagagaat taaatactaa aatagatgaa ttaaatgaag aaatactatt
    29161 tttaaaagaa gagaataaag agtatgaaga agttttggag aagtggaaaa atgtaaaaga
    29221 agaaattaaa tccgcatatg aaattagaag aagattaaat agaaaatatg gaaagttaga
    29281 aatgatatgg cgtaaaatcg aggatttata atgaatacaa ataaaatata tgagaaaaaa
    29341 gaatatatta ttattaaatc taaaaatgga tatattgttt acaatacaaa taaaaaattt
    29401 gaagaaggtc atactcattt aaaaagttat aacgctgcta aaacagctat agatttagtt
    29461 attaagagaa gaattcctag aagttattct atttattttc ttacaagttt gatgagaata
    29521 tctaatgatg tacaatatat aaaacatata agcgatattg tatatataag aaatagtaaa
    29581 ggactaaaag aaaaatatat aaattctaaa aatattaaaa aaaactattg agaacgtaga
    29641 aggtatatga tatactatga atatagttaa aacagagaag gtaaaaagag gggtgaataa
    29701 agatgttaat aaaaggagat aaaattaaat taataaaaga ggtggatggc ttaagagtat
    29761 ttaaagtagg tgatatattt acagttataa atatatataa tgatggaact atacatttta
    29821 aaacagatta tggaatgggg tttatgagtt atagtgaatt taaggagcat tttgaaatag
    29881 caaataataa agaaaaaaga aaatatactt ggagtaattg gacaacaata gtttctccta
    29941 atctacatat attgtatact tataaaacca acaataaaaa agtgatagtt aaaaaagatg
    30001 gagttaaagc ctcagctact tgccatgata cagatgtatt taatttacag aatagtgcgg
    30061 aaagcccact cctttaggtg tgggatggat agcacaaaca gtgtataaaa agtattgaca
    30121 catacacgta taatgttaat ataagtatat ggagaataaa tatagaagaa ctcaaacaac
    30181 tgttagttta attaattatc actttgtatt ctgccctaga tatagaagaa aaatatttaa
    30241 tatagaaggt ttagaagata gatttaaaac attagttaaa aataagtgta aggaaatgga
    30301 tatcgaaatt gttgcaatag aatgcgataa agaccataca catatgtttt taaatagtct
    30361 tccgactcta agtccttctg atataatgca aaatataaaa ggctatacaa gtaaaatatt
    30421 aagagaagaa tttattgaat tatcaaaaat gcctagttta tggactagaa gctattttgt
    30481 atctacagca ggaaacgtat gtagtgaaac tattaaaaaa tatgtggaaa accagaaaac
    30541 aagatactag aaagtgaggt gaacactatg tcaaattata tattaacatt acaattaaaa
    30601 acagaaaagt ttcaagaaga cattatatgt aatgcttttg ataagtatag aaaagtatat
    30661 aattcatgtt tatcagaact atttaaaaga tataaccaca tgatagaatc caaagaatat
    30721 caagcaaatt gtaaatataa gggtaaggat agaaataaaa tatttagtga aattaataag
    30781 aagtataatt taacagagta ttcacttcat aagtttgtta aatctattgg taaatattat
    30841 aaactacatt ctgctataac tcagaagata gctacaagat gttttaatgc ttttagtaag
    30901 tatatctatc atcaagctaa acaggttaat tatattagat atagtgaatt aacatcaata
    30961 gaagggaaac aaaattcaac tggtattagt tataaagatg gtattattaa atttaataag
    31021 atgactattc cagtaataat aaagaataat gacaactatg ctcaaagagc aatacaagat
    31081 aaaataaaat attgtagaat acttaaaaaa gaaattaagg gtaaaattaa atggtatgta
    31141 caattagtct tagaaggaat tccacctaaa aaagcaacta atcagggaaa aataaaagga
    31201 caaattggtt tgggtaatgt aggtatagat attggaactc gaactatagc tatatccagt
    31261 aaatctgagg ttaagttatt ggagttagct cctaatgtta ataatataga taggcaaata
    31321 aagttaattc aacgtaaaat ggatagaagt aaacgtgcaa ctaatcttaa taaattcaat
    31381 gataatggta ctataaaaca aggaaataga gataagtgga tatttagtaa tcactatctt
    31441 aaattaaaaa aactaagaaa agagttatat agaaaacaag ctgaaataag aaaacaagac
    31501 cactatatat taattaacaa attactaaat ttaggcaata agttttatgt tgaaacgatg
    31561 agttataaaa gacttcaaac tagagtcaag gatactacta ttaatgaaaa aactggtaga
    31621 attaataaaa agaaacgttt tggaaaatca ttagcaaata aagcaccatc aatgtttttg
    31681 actatgttag acaataagtt aaaatataat aatgaaagat tatataagat agatactgct
    31741 aaatgtagag ccagtcaata taatcacttt tcagatgagt acaataagaa ggaattaaaa
    31801 gatagatgga ataatggtat ggacattcaa agagattgct attctgcatt tttaataatg
    31861 aatgttaatg aagatttaaa atcaatcaac agagaattgt gtgttaaaac atatgataac
    31921 tttaaaatat tacatgataa agaaataaat agattaaaaa aattaaaatt aaatggttat
    31981 aaattaatat ctagtatggg gatataaaaa ctatataggt tgagaaacga gcctaatatt
    32041 aatgttaatg gtgtcaacag atactttgtt aataaaagtc ttagggaata tgattagtga
    32101 taatatgttg taggttatga tttattataa ctgagagtat tgtagaaatt catgtagtac
    32161 ctaagaaccc cacgccttta ggcgtaggag tttcagatta aattatctac aaataaaatc
    32221 tatgataaat caaagagaaa atcataaaat ggaggaatgg agaattctgt gtgacttttt
    32281 taaaacacta ccaaaatata aagaattaat gatgtaatag ttttgttagc aattaaattt
    32341 taaatataaa ggagaagtgg gtatggcaga aaaaagaata aattataatc aagtatttgt
    32401 atcaggagaa gttttagaca ttttaaatat agctagaatg aatcaaggaa ctggccaaga
    32461 agctataaga tttaccttaa aggttgaaac agcaccaaac gaatcagtaa atgtagatta
    32521 ctttacatca atgtttaagt cagatggttc accaaatcaa atgtttttag gaatagagac
    32581 aatagctaat gaaataaaaa ctagagcaga agatggaaaa ggtgatattg ttagatgtgt
    32641 ttgttcatta gacaataact tatattataa agatggtgaa aagaaagagc gattccaaat
    32701 atcaggaact ttttgcaata gagaaaaata tgatgacaaa ggtaatccta ttgatgacaa
    32761 aggcgaagta gtaaagtcaa taaaagcgag tcaggtctgg agagtatata cattaattga
    32821 aaatatagaa gaaaaagaag atgatttagg taagtattta gagattacgg gtttaattaa
    32881 taaatatggt aagcaaggtt gtaatatgac atttagaata cataatgaag atatgataga
    32941 aggatttaaa agtttatata aaataggaga tgttggcctt cttgaaggta cagtaaaatc
    33001 tatggttact agtaaatcag caggattcgg ttcaagaatt aaaaaaagtg cttttacatt
    33061 tttagaaata gaaggtggtg atgagccttt aaaagatggt gatgaaatat taactgataa
    33121 aaattatcct ttcacagata agaatataga ggcaatgaga gaaaaaataa aagaaaagaa
    33181 tgaaaaagaa aaacaaagag atattgataa aaatggtaag acagttgaag taagtgatga
    33241 cgatgtacca ttttaaatta taaattaaat aatctattaa aaaggagaga tgtgaatgga
    33301 agttaaagaa gaaataatga gtgatgattt caagaaagtg gtaaatgagg aaataaagaa
    33361 gtatatatca aatttacatt cagatagaat agacggatta aaagagagtg atagtcaaat
    33421 tgagataaca aaagcattaa tagcctttca tttagatata gagtcaattc caaaagacaa
    33481 tacaaatcca tttttcaggt ctaaatatgc aaaccttgat acaattctta aagcagtaaa
    33541 tccattatta gcaaaacatg atttaatact aatgcaatca gcagaagatg caggtaatga
    33601 taaagtatat ataaaaacta aactaaagca ttcatcaggc gaatacatag agtcaaattc
    33661 tgcaccattt aaaccagcta aaactaatga tatacaagct agaggggcat tggaaactta
    33721 tttaagaagg tatgctgttc aatcagtatt agcattatca ttcgaggaag atgatgatgg
    33781 aaattcacta acaaacaaat caaagagcaa atcagaggaa gaaaattcaa ctgttaagaa
    33841 aagtagagtg taagtaaaaa aatgctacct cttaatgagg tagcaataaa tatatagcat
    33901 aaggagggga aaatttgaat tgtaagtgta aggtttgtaa gaaaaaatta aatacaaaag
    33961 atgcctataa agtagaacat attacatctg gcggaaataa gcagaataga tattactgta
    34021 atgaacagga atatagaaaa gagcagcagg acatatattt ctggaaacaa tgtcaacttg
    34081 gtatagatta tattatgggc tatacagtta taagtaatca aaaaaataag atgttacaag
    34141 agataataaa aaatggttat acaagagaag aattatatga ctgtatgctt gaaaaaaaag
    34201 atgaaattat agaattactt aattacagaa aagatataga agaagaatat cctaaattat
    34261 gctatgtatt tactatatta aaaggttgta ttagagatat aacaattaga aataagcaaa
    34321 taaaagatga aaaagaaaat gaaaagatat acaaagaaag tgaaaaatat tatgaagtaa
    34381 ttactccgaa aaaggtactt actaataaaa gaaaaagtct atttgaaaaa attaaagagg
    34441 tggattaatg gaggcatatg aaaaaataac aaaggataga ggtattatag agactttagt
    34501 tttaggaaca atgctaaaat cattaactct ttttagtgaa tataaaatta gcgaaagtga
    34561 cttcataata gataaagtaa aattcttttt ctcattagga agaacaatgt ctaaaaccca
    34621 tagtgaactc gatgaagcaa gtgtagctaa atttgtatct tcaaataaac ttaaatctga
    34681 atatgaaaaa tatggtggtt ggaatagctt atcatctgcc atggagtatg gaaaagaaac
    34741 taatatatca gcatatattg atgatttagc taagaataat ctattaattg ctttagataa
    34801 aagaggtttt aatataacaa aagagatgga acataatgga ttaaaattta ttccatttga
    34861 gttattccaa tctatgaatt gcagagaagt ggaagaattt tatgaagggc taatatcttc
    34921 atgtagtgta aattccataa agaataatat gaaagtcgaa aatcttttac ttaccaaaga
    34981 agatagagaa aaactaaaac aaaaaactga agcaggaaca ccttataata tcatttttga
    35041 atatagtgaa aaagagacag ggttaagtga tagtgaagaa tctaagtata tatatggttt
    35101 accagtatta tcaaatagaa ctaatggact tggtaaaggt ggaggaataa atataatatc
    35161 tggattttca ggaataggga aaactacatt attgttcttt aattatatat tagctatgat
    35221 atatcaaggt gaaaaagtag ttatattcgc taatgaacaa aagtctcaat attttaagag
    35281 tatgttggta tcatttatag catacaacat atttaattat catgatttag atagaaataa
    35341 aattgataat ggagatttta cagactttga agaagaactt atggaaaaaa tagaaatctt
    35401 tttaaaaaat agaggctttg aagaaagttt aaaatttata tatatggaag agtttgaaat
    35461 atctgagata ttaagaaaaa gtaaagaact tgtagcacat gaagggttta ctgtaatagc
    35521 agttgataca tttaagtcag agaattcatc agatgcaaat tatacaggaa ggctaataga
    35581 gaactccaag ttactagaca actttgggaa taaatataat gtaataacgc tactatccat
    35641 gcaattagta acagctcaag agaataaaag ttcttactta tcagcttcag atttagcaga
    35701 agctaaagct gttaagaatg tgtgtggtct attaatgtta atgaggaaag tagtaaatga
    35761 cttagagtta gattcaacca ataagaaatt ctttttaaaa ccatatagac taaaatataa
    35821 taaattaaaa aagactactg aaagagaata tatccaattc gatgtaaaag atttacaaaa
    35881 agaatacaga ttattatttt taaataagtc aagaagagga caagatggag atgtaatctt
    35941 gttaagattt tatggtaaga ctggtagatt tgaagagata ggaaggtgtg aaaaggtcta
    36001 tagaggacaa ttgtcatact aggtgatgac tatggaaata aaggatttaa ctaatgaaca
    36061 agtgataaat tttatggaat atttaggttc ggacttatct cctaaaagca atgatagaca
    36121 gttaatcttt aatacttgtt tatgccataa tggtgattca tataaattat tttattatac
    36181 agaaagcaaa acatttcatt gctatagttc ttgtggtcat ataggaagct taatagattt
    36241 attaatacac ataaataaat atgattttaa agatgctgtt aatgaaataa aaaatttctt
    36301 tggtatttca aatcaaccaa tgctaagaaa aggatttaga ataaagaaaa aattaaaaca
    36361 agatgacatt agagatatac aaatagaatt acttcctacc tctaaaaagc catatgtata
    36421 taaaatattt caacaaattc caattgaaga atgggaaagg gaaaacatat cttttgaagt
    36481 tcttaaaaca tatcaaatac attataatcc atatgaaaat caaatagtaa ttccacattt
    36541 ctgttggcat gacaaagata gacctgttgg tattagagtg agaaatttag atgaagataa
    36601 agctaaaagt tttggcaaat atattccatt atggtatgac agtaagtgtt ataatcatag
    36661 acttagtctt aatttatatg gattaaatat taatgaacag gccataaaga aacgtaaaaa
    36721 agtaatagta tttgaaggtg aaaaatcagt cttgcaaatg gccactatgt ataaaaataa
    36781 tccatctgta gctatatgtg gaagtaattt tagtagagag caaaagaaaa tattgctaga
    36841 tttagggatt gaagaattaa taatagcttt tgataggcaa tttaaaacta aaggagatga
    36901 agaatatatt gcatggaaaa ataaaatatc caagttagtt caagatatta aagatgtagt
    36961 aagtataagt gtaatttggg atgaagataa tttattggat tataaagata gtcctagtga
    37021 taaaggtaaa aaaatatttg aaaagctgtt aaaaaataga gttaatataa tacaatttga
    37081 aggagtgatt aaatgagtga agttataaga caagtagtag atgaaatagt tagaataagt
    37141 agtttagaag ttgctaagat gatgggaatg acatctaaag atgggcatag agatttaata
    37201 agaaagatag aaaaaataaa tatagttttt gattcggaaa aaattccgaa tgaaatatat
    37261 tggttggaag gtgaatatga gaatagaggg aaattctata aagaatattt agtatctaag
    37321 aaaggttgtg aattccttgc acataaaaca actggagaaa aaggggtatt gtttaccgct
    37381 aagtatatgg aacgatttga agaaatggaa agaattataa aagaacaaaa acttgagtta
    37441 tcagcaagag ataaaggaat attagatata gtaaattcta agtcagatat agaacaagca
    37501 atagcaataa aaacttttga agaagttgta actgcacctc ttatagaaac gatagaagaa
    37561 caaaagccag atgttcaatt tgcgaatagg attagaacag atggaagaaa agtctatagc
    37621 atatcacaag cttctaaatt attgaaactt ccatatggga gaaataaatt aatggaaaat
    37681 ctaagaaatc taaaaatact tcaattaaac aatgaacctt atcaaacata catagataga
    37741 gattatttat tagtggaact aaatgaaact ggaaacaagt tggttgtatc tcctagaata
    37801 actggtaaag gaatgaagtg gttagaaaat aaattaagag ataaattact tacattagca
    37861 taataggggg tgattatatg atacaggaag ttagagaaga agtgttatta caaaatggcg
    37921 atatattaca tacaggagat attataaatg tagaatataa aactatggaa gatacaaaag
    37981 agcaattttg taagggtaga ttaaaaagtg tagatgagca atttatacaa ctagatacaa
    38041 gtgaaaaata taatgcagca gaaaaaacaa tctatgcatg ggatttaata gagataaaaa
    38101 aggttggtga tgaaaatgaa aaggataatt aatagtaagt atagagcatt gtatttattt
    38161 atgttttttc tgatttttaa cttaatatat agtttatatt ttggaaggaa tacggagatt
    38221 ggattttatt ctagtacatc ttgcatagaa gaatatatac tggatattat aacctctata
    38281 ggactatttt tctcaatgat gttagctggg tttgatgtta cagaaaattt tataaacagt
    38341 ttaagggatt attgtgaaga agatgcaaag tgaggtgaat aaattggaat taaaggatta
    38401 tataagtaaa gtagaattaa atgatatgcc actaacttta gataatcagt acatgttaaa
    38461 tgaaataagc atatctgaat atggtaaaat atttgctgta aaagacacat caggaagaac
    38521 atcttattat actgaaatag aatttaataa actatttaga ggaattaata gcatcctaga
    38581 gaatattatc taatatatat ttttaaggaa ctgagggttg agagctatta attaatagct
    38641 ctctattttt tgaaagggga gagttaatgg aaaaggaatt atatagcttt agcaaattaa
    38701 atgcatttca cacatgtcca tttcagtact atcttacata tgtaaaaaat ttaaatcgag
    38761 aacaaaattg ttatggttat tatggtaatg aattacatag tttactagaa caattacaac
    38821 aaagaaaaat aacaaatcaa gaagctattc aaaaatataa tgaagttata gaatatgcaa
    38881 atctaatgga ttacaacttt cctacaccaa attcaagaat taattattta gaatgtatct
    38941 tacattatat agaaaatttt gtaccaattg agtgtgataa atactacata gaagaatact
    39001 ttgagtttga cattaatgga attacaatga ggggatatat agatttatat tatataatag
    39061 ataataaagt ttatgttata gattacaaat cgagtagtaa attttctaaa aaagacctac
    39121 ctaaaaaatc aatgcagctt atcttatatg caatgtatct taaagaaaaa tatccagata
    39181 aaataattga atatgtagct ttcgatatgt gtaagtatgt ggaaaatgaa aaaggaactc
    39241 taattgaaag aaatagaatt ggaaatatag atgattacga aagagctata gtaaaaatta
    39301 aatacactaa agcattggaa caacaattaa ttgcctttgt tactgataca gttaaacaga
    39361 taaaacagct agatttcaac gacgagagtg tttggtgcaa aagtgatgac aaaagtaatc
    39421 agtttttttg caagacccta tgctctcatt ataacaaggg ttgcaaacat aaggaaaaca
    39481 agagttttag aaaatcaaaa tcacaataaa atttgaattt taataagatt ggagagtgtt
    39541 taaatgaata taccaaagca agtaaaagta ggaggtttat tctataaaat agaagagaca
    39601 gaagaaccta ttttagtaaa taatcaatta tgttatggat tagctgatta tggaactgaa
    39661 acaataaaat tatctaataa tctacaatca gaaagagtaa aagaagctac tttcttacat
    39721 gagttattcc attgtatatt tagagataga tgtatagaga gtgaagatga ggaatattta
    39781 gtagatgtat taagcaaggg acttcatcaa gtgattattg ataatcctaa tatatttgaa
    39841 cttaaggtga gtatagatgg ataaattaaa aaatatattt gtatttttag gttattctgg
    39901 ctcaggtaaa gatagtatag taacagaaat ttcaaagcaa ttcaatattc caattctaat
    39961 atcacataca acaagacctc cccgtaatta cttagaaatc attaataaaa cctatcattt
    40021 tgtagataat aaattcttta agaaagaaaa aaataacttt atagaaatga gaaagtatat
    40081 agttcatgat ggcagcactt ggctatatgg tatacataaa tctgaattag agaataaaaa
    40141 atatgctttg actatagtag atgcaagtgg atataaagca ctagaaaaat attttacagg
    40201 aacaaaaact aagttagtac cattttttat aaacgcaaat gaagatacta tcagaaaaag
    40261 attaatagat agaggtgata atcttaaaga aatcgaaaga agattagttg atgataagat
    40321 taaatttaaa gagtttttaa atagtgaaaa ctacattgta atagcaaata acttaagtat
    40381 aactaatgca gtagagcaag ttaaaataca tatgaaagag gggattcaat ggtcttagcc
    40441 ttagatgtat ccatgtcctc aactggatgg gcagttataa atagaaataa gaatatatta
    40501 aaatatggaa aaatagtaac taaaaaagat aaattcaagt ctgaagatga gcgaatgtgt
    40561 tatatctgta atgcaattca agagttaatt ataactcata atattcaaat agtcttaatt
    40621 gaagaccaat tcacttcaag aaactctaaa acaattcttg gtttaagaaa actcttaggg
    40681 gcaataatga ggactgtaaa attaaataat atagagattg aatatatgta tccagtttct
    40741 attagaaaat atcttatgaa tagtggtaaa gcaaaaaaag aagaagtagc tgcgtatgta
    40801 agagaaaata ttattgatat tggagagtac attgatagga cttgtaaagc aaagaccagt
    40861 gatatatatg attctatagc tcttggagta gcatatttga ataaggttgg tgatgttata
    40921 tagaaaatag tatgtacaat cttaaagcgg taaaatttga agaattgact aaaaagtata
    40981 aattagaagt agcaccatat agggaaatgg attctaactt tttcttagta tgtagaggta
    41041 tatttgtact taaagatagc aaagcaatgc atttatatct atatttatgt aattgttata
    41101 atagagcaac tgaaaaagtg tttccttctt acgatacaat acaaagagca acaggcttaa
    41161 atagaaatac agtatctaat aagttaaaga tactacagaa attaaaactt ataaagatta
    41221 ataaaagaaa agtaggtaca ggatttaata attattattt aataaactat ataaaatcag
    41281 taattgagaa aagtaaaata aatgaaaatt tagagataga ggaaataata gatatggata
    41341 attgtttagc tgatttgaag ttagatactt ccattcaaaa ttaaaagtga gtatcaaatt
    41401 taatactagg gtagtagcaa ttttaatact accctagtag cagaagtgat actgtaacaa
    41461 taagaattaa caataagaat taacaaaact aatattacgc attagataaa tctaatgacg
    41521 attttaagtt taaattatat agggagtgat taaattaatg agtataaatg taaaaataaa
    41581 gaaattaagt caagatgcaa tagtaccatc ttatgcaaaa gaaggtgata gtggaataga
    41641 tttatataca ttagaagaca cagtaattcc agctaaatca gcagtagcaa tttctacagg
    41701 tatagcatta gaaattccat atggatatga aatgcaagtc agacctagaa gtggtataag
    41761 cttaaaagga atattattta caagagatgt gactgtatgt gataaagagc aacttaaaaa
    41821 acaaattaga aaaagttttg tagtacatcc aactgtaaga ttaggtacag ttgattcagg
    41881 atatagaggc gaaattaaaa taattacata taatgaagaa aactttgaaa ttctaattcc
    41941 caaacatatt aaattagctc aaggagtatt tcaacaagtt ccaagagcta tattaaaagt
    42001 tgtagatgaa ttatctaact ccgaaagagg taatagtggt tttggaagta ctggtataaa
    42061 ataatgccta aatatataat cctagaaaaa tacaattatt actatgatga caagtgtcat
    42121 actttatatc atttagaaaa catctataca ggattaagat tttctattga aaaaaatgaa
    42181 ttagagtact ataatatatc ctagactaac tttgggagga gtattaaatg tacagatatg
    42241 atgattatgt aaatttaata aatgacttag aattgccaaa agagattagg aataaatgtg
    42301 ttagagttat aatgacgcta gataacatgc agggcataat ggtaatacat aagtttaaga
    42361 cttatgttgt taatgaaaaa tatattggaa aagtagcata aaataattaa gtcatctatt
    42421 aatttaggtg acttttttgc atttatgtac tactatttag gtaaaggtta atttaaacaa
    42481 atgaggggga tatacgatga aattaccaaa aaaattatta tcactagggt tagttataac
    42541 aatgctaata attagttttc caaaacaatc ttatgcactt aattcagtag atgagattga
    42601 agtatctaca atatatgaaa cagcaggtct tatagcagat aagcaaagtt atgacactgc
    42661 aatactagtt aatttaaatg attccatagc agatgggtta agtgctagtg gcttatcagg
    42721 aacattaaat gcaccattgc ttttatctga aaatagtagc ataccaaatg aaacaatgaa
    42781 gagattaagt aaagtaaaaa cagtttattt gataggtggg ataaaatcaa taagtaaatc
    42841 tgtagagaat acattaatgt caaaaggatt taatgtaaaa agaattgatg gagatgatag
    42901 aataaaaact agttataatg ttgcaaaaga aataaattct aaacaaaaag ttaatactgt
    42961 aatgctaaca aatgcttata aaggagaacc agacgcaata agtatagctt ctgtagcggc
    43021 aagagataag gcagcgataa tattaacaga tggaaaaagt ataccatttt caactaatga
    43081 agtaaaatca tatgcaattg gaggtacatc tacaatgaat gatacattag ttaaaaatac
    43141 aaattcaaca agattggggg ggtctgacag atttgataca aataaaaaaa taataaatca
    43201 attttataat ggtataaaag agttttacat agctggttct aggaacttaa ccaatgcact
    43261 tttagcatca tctttaaaga gtccagttgt tttagttgat aataatagta ataaatcaat
    43321 attaaaaaat gccactaaaa ttacaacagt tggagacata acagaggagt tactagaaga
    43381 atgtttgaat gttactaatg gaataggaga ttctaacact ggagtaatta aaaaaactag
    43441 acttgaagaa gctaaagaat tagctaaaga ttggtataat tgttatgaag taggagatat
    43501 agacaatgta ccatatgatg gagtatactt agttgatgat aattataaaa atggaagtaa
    43561 ttattattat gtttttagtt atgtgtctgc tggtcaagag cattccgacc gtctatgtat
    43621 aaataaaaat gacatgtcag attataaact tttatatgaa gatttacata tggaaaaagt
    43681 ggtttcattt aaaaatgctg aagatttagt aaaagactta tttatgaaag aacatggatt
    43741 tttaccaaac catgtagaag ctcaaaatat ggaagatggt atgtatatat atgtacgtcc
    43801 atttaataca tatgtagata tgaatggtat agagcataat gcaggtggat atacaccata
    43861 tttagtcaat atttatacag gtgaaatatc aaaatataca tatactgaat aaagcaatta
    43921 atcaactact ttttaaagta gttgatttta ttttaaataa taaatttatt atatcactta
    43981 aggcatatat agctgttata attaaaatat atgtcgaaag gtagatggtg aggatgttta
    44041 aggagatatt tgaaaaggta tttatgtcag aagaggttag taaaactgta aaagaagtta
    44101 ataatattat taaggatact aagatacaag taaaaggtgg atttggagaa cttagattag
    44161 attttatact tagcagatta ggcaaagatt acataataat taaagacatt attgtaccag
    44221 gctcaaataa gactacacaa attgatagta ttgtgatttc tatatatgga atctttgtta
    44281 tagaatgtaa aaattttagt ggctatatct atggaaatga caaagataaa gtatggactc
    44341 aaattgtagg aaaaactaaa aatacattct ataatcctgt aagacaaaat tatgctcata
    44401 ttaaagcaat agaaaatata ataggagata aatacaatat atattcaata gttgtttttt
    44461 cagacaaagc tactctaaaa aatgtaatga taagtggaaa taatattaga gctataaatg
    44521 aaagtgaaat attaaggaca ataagtagat ataaagatat gactattgat aaagaagaaa
    44581 taaaaattat cagagataaa atttttgatt gtatgaaaaa cactaatcaa aatactagag
    44641 agcatgttag aaatttaaaa aatatcactg aagaggataa gtgccctaga tgtcaaattg
    44701 gacatttgat taaaagaaaa ggtcagtatg gtgaattttg gggttgcagt gaatatccaa
    44761 agtgtaaata tactagaaaa taatatattt atactttgga tattgaaata aaataaaact
    44821 tcactctaat tagaatgaag ttttatttta ttatttgttg gaattttctt ttttattaat
    44881 ataggataaa taataactat catcaaaaat aattaatata cattctaaaa ttattataaa
    44941 atttccaact ccataaacag tccaatattc tttaaacaaa taattaccat tgaatattct
    45001 atttacaaga ggtgctgaaa aatatatagt tccaaatata gatattaata tcataactga
    45061 tataaataat atattgttca aaaattgatg tcttttttct cctctcttta gagaaagaac
    45121 taaaactaat acagatattg cggaaggaga atatttaata taaatctcat aataatcttc
    45181 aatatgaatt tgcataaaaa gctctacaag tatatggatt gctactgtta aaatagttat
    45241 agttgataat atatttcctt gtctaatttt ttttcttaat ttaaattcat taattttatt
    45301 ttcaaaattt ttctgatagg tatttttatt agactttaga acagatttgc atttaggaca
    45361 tatagttgta gtatcatcaa attctaaatc acagttttta caatacatga atacttatgc
    45421 ctttttttcc atatacattc cattctcttc catccataaa ttaaatcttt ctgcttttct
    45481 tgcacttcta gaatattgta gtgcagcata attagctttg tcaaatatat ctttaataac
    45541 catactatat aagtttactt ctgccaacat tgccataact ggaggagtaa atgttagtgc
    45601 tgagaacact aatccaagca ccatatatcc tatttgttca ttgtaatctt tatattttat
    45661 tttcatacta ccttgtacat cttcaattat agcttcaggt gtatcatgtc tagaataggc
    45721 atctttaaaa aatataaaca tatcattctc taccatagat tttacagatt gtacatctat
    45781 ttcaaatgat ttcatatcaa ctattgtttt aactacagaa gctttttctt tagcaagtgt
    45841 tgtatataat gaaggtaagt caccttttaa tgctattaaa tttgttataa ctgaacgcat
    45901 agaattagca cttctaaaag cagaacctct gcttaaatat ctatcataag cttctctgtc
    45961 atatttagtt atatatgtac ataaaccttc ttcataatta tctccttcaa aatctacaaa
    46021 atgagataaa tacatgtata ctacctcatc attatacttt ggtgctccta cttcttgatt
    46081 ttttctagct gctgacattg catatgataa ttgacttgca ttagtcgtat gtttaatggg
    46141 ctcttcacta taagtcttac ggatgtccat atttttttct aaatatgaat tacatttttg
    46201 tatgaaatca cttcctttag gtgtcaaatc ttttaattct ccaactgata tgccaatatc
    46261 ttttaatgcg ttactgtatt cctctgatac agtaagtaca tctgcaacag tgttcatatc
    46321 catatctgaa gattttgcag atatagtata tgaatttact gttgttgata taagaaaagt
    46381 agttgctagt gttaaactta atacctttaa tttagttttt ttcatttgtt ttccccctca
    46441 atatatattt aattaaagtt ggaatattct tgaaattcca actttaattc aaaatgactt
    46501 tatgaatgat attgtagtga taatatgatt tatataaaag gactagctat ggatttaaga
    46561 gtgataattt aagaaatgta ttctatacgg gtagtatagc ataaaaagtt aaagcaagaa
    46621 atatataaaa aaaataatat ataaatataa attaatgtga taatttatat ttatcagatt
    46681 taaaatatta tattgattat ttcaatctga taaatattga gcttctaaat tccattttaa
    46741 ggctttgtaa aaattaatgc ttataattaa tcctattcag aaatttgatt gcctaaaacg
    46801 caaaatataa aaaaataata aattgctagt ataagacata gaatcatgca ttctatgtct
    46861 tatttcttta taattaagta atttcaataa ttatacaaat aaaataatta aaaaagtcag
    46921 ttgaaagtca gctaaattga ctattttttt tgccttatct gtatggtata ataaatatat
    46981 aaattttgat aggagggatg atgatgaacg cttttataag aaaaaggaac aaaaactatg
    47041 tagtgtactt agaatttaga gatgatgaat cagggaagag aaaacaaaaa aatatggggg
    47101 cttttgataa aaagagagat gccaataaaa gattagctga agttaaagac agtatatata
    47161 aagatagttt tctcgttcca aatgaaatca cactgtctga atttttattg gattttcttg
    47221 aaaagtataa agataatatt tcagcgtcta catataaaag ctacattgct atttgtaaga
    47281 atcatattaa tccttctatt ggaaaatatc gtcttcaaga gttgagaaat attcatatac
    47341 aaaattatat agatgattta gctggtaact taaatcctca aacaattaaa gtacatataa
    47401 atgtattgag acttgccata aagagagctt atagaattaa attgataaaa gaaaatatta
    47461 tagatggtat agaaagtcct agaattaaaa aatttaaaaa tgaaatttat gataaagagc
    47521 agatgctaaa attattagaa gtagccaaag gaactaatct tgagcttcct attagtttag
    47581 ccacgggtct aggattaaga ctttcagaag ttttgggatt aacttgggac aatattgatt
    47641 ttgatgaaaa tacaataaca gtaagtaaga taactagtag attggatggc tctgttatgc
    47701 ttaaagagcc taagacagaa agctcaatta gaaaaatatt cgcaccagta gagcttatga
    47761 atttattgaa agattataaa ctggagcaaa ataaaaaatt attaagaagt attattagaa
    47821 atgaacataa cttactattc tttgatagaa aaggaaatcc aatagctgaa gatgtaatga
    47881 gtaagaaatt tagaaaattc ttagagaata atgatttacc tcatattaga tttcatgatt
    47941 taagacattc acatgttact ttacttataa attctaaagt acctataaag gttatatctg
    48001 aaagggtggg acattcaaat attaatacta cacttaatgt atattcccat gtgcttaaag
    48061 aaatggataa agaagcttca gatagaatat ctgaaaactt attcaaagct aattaatggt
    48121 ataatatata ataggttatc aaaggaggcg gctatattct atgggaacaa gtaagatttc
    48181 aaatatacac aaggaaaatt tatctattgc tgagattcaa aaattatgtg cgattgcggg
    48241 aaatattgaa gcaaatattc aatcaaatga caaaacacca tcttgggacg gagaactatt
    48301 tttatatgaa aaaaataaaa ttgagaagga aaattgttta gataataaaa aagaaaattt
    48361 attaagaaaa ataaatatac aacttaaagc aaatgaggta aaaaagttaa gtggtaaaaa
    48421 aagaactttt tcaatggatg ttagtgattt aagaaactac tacaataatg aaggtgttat
    48481 tttatttgtt gttgaaatta aaagtgttaa caaaataaaa gtttattata gaaatttact
    48541 tccagttgat ttgataaata tattaaatga aatagataga acgaagaaaa atcaattgac
    48601 caagtctgta gagctatatg aactaagacc tgaaaataat cattttgaaa gaatagttaa
    48661 ttcgttttat cgtaatataa ataagcaaac aaaaaattta gtagataaac aaatagaact
    48721 aagtgaaaga gacaaagaga tttcaataga tattgcagaa atagataata gatatgattt
    48781 atttaataga agtgtgtatg cttacaaacc acttaaacat gaagatttag atacaattga
    48841 tttaccttgt gtgaataaat tgtatcttaa agaattaaat acaaaaacta ttgtagatat
    48901 ttttataaaa aataatattt ataaaaataa tgaatatata tcaacagtaa ataaaaccaa
    48961 tcacaaaata actatcaata attttattgt tatttatatt taccgtatat tagatgagaa
    49021 attaatcaat aagtctattg atataaattt tacgataaaa gaaccaagtg gaactgttga
    49081 tagtcattta aataatttaa agatgttatt agatatattt aaacataaaa aagtacttat
    49141 aaaagaattt tcaacaaaag atgaacttat tgatttagat ttaaaaacta tgccatgcga
    49201 agaaaaggat ttaatagaaa atatattatt ttttgaagat tttaagaatc tgttagagat
    49261 gcttgaaata aaacaagaaa atttcttatt agataatatg tctcaggaag attatgggaa
    49321 aataaataca cttataagta tatttattaa taataaaaca attaaaaaaa agaatatgag
    49381 taaacattca ggatttgcat atatgaaaat taatgaaaag aaaataatct tatataagat
    49441 agaaacagat aatagttgga atcttattaa tatttttgat aaaaaaaata accttactat
    49501 tactcaaaga gaaggagata tgcaatgcag tccatatata cttttaaatt ataatgatat
    49561 ggatagtatt aatttcgatg taaataaagc aactataaat attaaaaaat ataaaaaaac
    49621 accagagtat acagatagag tcatgtcata tattttagaa ttaattaaat actatgataa
    49681 aatacttatt aaagaatatt taattgaggc actagagtta ttagaatgga tagaaaatga
    49741 agttgatgta gatataaaca aaattaataa atatcaaata ataaaaagga ttagaaattt
    49801 tagcattgat gaaatacttg atttagataa tattaaaaga aaaaactcaa aagacataat
    49861 gattcaatgt gcgataaata tattaattga aaagtatgaa gaagctaaaa ataatatgaa
    49921 caatatgagt aaagaattat tgaataaatt taaattatat ccaatatata atttgtatga
    49981 taaaaaaaca aaggtacaaa tataaaggta aatttttata aagccttaga atggcgttta
    50041 aagcgtcaga ttttagaaaa tattaaaatt ttatatttcc aatatttatg ctataataaa
    50101 catagcaagg aataaatatt tgaaaaagct atgagtggtg tttccataag gatttcctcc
    50161 ctttcttcca agaaaggagg tgaaaaacta tggaaaatct aatgatgagt attatagctg
    50221 gtgttatagc tagttatatc tacgataaat taaaaagcca ctctgagcga ccaactaaga
    50281 gtggctggga acttaatatt aagttccata aaaacaaaca ttaataacta attaatggaa
    50341 acatcactca aagttaagta aaatatgttt ccttgctttt attataccac aaattaagaa
    50401 tattaaaaca aaattttgta aatacatact taattatcaa tttaatcatt tggaaaaaat
    50461 tggatttgat tgtatgaaat tattgctgta atatttaaat taaataaaca aatgatggga
    50521 gagataatta tgtatttaag aaaattatca aagtcagaat taattgtaat gaaatttata
    50581 tggaatttag atacaaaagt aaaatcatat caaatcatta attatatgaa agaaaaatat
    50641 agttggtcag aaaaaacaac attaaaaact ttatctaagt tatcaaataa aaggttcata
    50701 tatgttcaag aaacaagtca atgtacatac tatacaattt caattaaaga agataagtat
    50761 catgaattta tatcacaaaa aatacataag ctcttagggt gcaattctat aaaaagcctt
    50821 ttagcatcat tgtttcaaga agaattaaca gatgaaaaaa taacttcatt ggaagaatgg
    50881 gtaaaaaact gggaagaaga ggaataaaga taatatatac tattccttaa aaatctattt
    50941 taagaatagt attaaatagt aaaatgatga ctatataaaa aagaactaat tatagtcctt
    51001 tttattttta aaattaatgt tttagtttta tttcatttac atcttcctct acatttaaat
    51061 gtaaattgtt ttttgatata ttcaactttt tctccatatt cttttacaga ctcataaatt
    51121 ccaccttctt caacctcaaa aaatcacata ttttctttat atcataccca tttaaacata
    51181 ctaaaaaaga tgtaggtaaa tttcttcaca aaaatatacc tacatcctcc aaaatactaa
    51241 tttttaaaat tcttatttct ttcatataaa atatatatac cagtaataag tagtacaata
    51301 aagtaagtgt atgtaacttt atccatccca ttaaaatata tcatagtaaa taaatttaaa
    51361 cttattaaaa taaatgaaaa gcaaaatttt gtttttgtca ataatcgcct acttttaaaa
    51421 ataattggta aaattataat tgttgaaata taagctataa aatgaaaaaa caattcatta
    51481 ttattcatta aatattccat ctatgtatat tcccttcgag attaatatct tttaccttta
    51541 tcagtaaatt ttgtaatgta tttaccatct ccataaaatg ttgtttctct ctcatagtaa
    51601 ttgtatttac catctgaacc atatcttatt ttataatata ttttaatttg gtcatactta
    51661 cctgcaactg tggcagcaac cgtagctgca accttaacag gagcccatgg tgcttttaat
    51721 gcaatcaaag cacttatagt agccgtacct aatccacctg tatttatata cattgtatta
    51781 gattgccatt ttccccattt aaccgcagct ttgaaagtat caatttcaga ttgttctaaa
    51841 ctattatcat tacttttgta attctcacta ctagtaatag aaatcttttt tccatctaga
    51901 tatacttcat tatctatatc ttctagtgta tttagaacat ttccatctaa tgatttaatt
    51961 gttattgtag ttttatcacc atctacaact ctatcatata taactcctag atttttatca
    52021 gtgtacgact ctgttgattt ttcacttgta taatatttat taatattatt agtttgtgcg
    52081 tttacagttg ttgttaataa tgtgagacta gcaataaccc ctattaatgc gagtgaaata
    52141 attttagtaa ctctcttttt taaactcata aacattcctc caatatacta ttttttaaaa
    52201 atattttaca atgctaaaca gaaatccttt taatattctg attataggag aggagatacg
    52261 tgttatttta ggcaacttga taataataca taatacaata atcgaatctt acatattatg
    52321 attattaagt ccctaattta attaaatatt tgatattccc tctaaaataa cttatatgta
    52381 aaagtgatta gatgctaaaa tatacttaga acatcaggtt tgggctataa aacaagcaat
    52441 tagtagttaa atttgcattt aaagaatact atcatagaaa catatcttgt tagattttga
    52501 gttaaaagct acattatttg attcatttcc tatctgaaat tatattgtat gtaaatcccc
    52561 tgaaaattta tggttaaata catattcttg attatcaata gtaactattt ctgtaaaatc
    52621 ttttttagaa gcattataag tattgttttc agttagataa gaattgtttg tctttgtaaa
    52681 attaacactc ctatctatga atgataattc aacctaactt aatcctattg ttgtcacaca
    52741 gcctaatgca attaatctac tctgtatttt ttttatcttg ttataccctc tacttcaatt
    52801 ctattttaat agaattttat catgtacagt aacaattggc aatataaatt tttaattagt
    52861 ttataaaata aaaatttaaa atatctaata tttaaaa
//
LOCUS       DAEORZ010000006        52109 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEORZ010000006 DAEORZ010000000
VERSION     DAEORZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.006"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(382..522)
                     /locus_tag="KRH39_000318"
     CDS             complement(382..522)
                     /locus_tag="KRH39_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510248.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
     gene            complement(782..1942)
                     /locus_tag="KRH39_000319"
     CDS             complement(782..1942)
                     /locus_tag="KRH39_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBG8510249.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFVTDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            complement(2113..2316)
                     /locus_tag="KRH39_000320"
     CDS             complement(2113..2316)
                     /locus_tag="KRH39_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBG8510250.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            complement(2403..3437)
                     /locus_tag="KRH39_000321"
     CDS             complement(2403..3437)
                     /locus_tag="KRH39_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBG8510251.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHHDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            complement(3960..5321)
                     /gene="rlmD"
                     /locus_tag="KRH39_000322"
     CDS             complement(3960..5321)
                     /gene="rlmD"
                     /locus_tag="KRH39_000322"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG8510252.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKSELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            complement(5348..6070)
                     /locus_tag="KRH39_000323"
     CDS             complement(5348..6070)
                     /locus_tag="KRH39_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG8510253.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGRFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(6070..6399)
                     /locus_tag="KRH39_000324"
     CDS             complement(6070..6399)
                     /locus_tag="KRH39_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBG8510254.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            6729..7655
                     /gene="fba"
                     /locus_tag="KRH39_000325"
     CDS             6729..7655
                     /gene="fba"
                     /locus_tag="KRH39_000325"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBG8510255.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(7790..9421)
                     /locus_tag="KRH39_000326"
     CDS             complement(7790..9421)
                     /locus_tag="KRH39_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8510256.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(9904..10941)
                     /locus_tag="KRH39_000327"
     CDS             complement(9904..10941)
                     /locus_tag="KRH39_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG8510257.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(10960..11745)
                     /locus_tag="KRH39_000328"
     CDS             complement(10960..11745)
                     /locus_tag="KRH39_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG8510258.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(11778..12911)
                     /locus_tag="KRH39_000329"
     CDS             complement(11778..12911)
                     /locus_tag="KRH39_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG8510259.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(12959..14086)
                     /gene="hadC"
                     /locus_tag="KRH39_000330"
     CDS             complement(12959..14086)
                     /gene="hadC"
                     /locus_tag="KRH39_000330"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBG8510260.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(14086..15312)
                     /gene="hadB"
                     /locus_tag="KRH39_000331"
     CDS             complement(14086..15312)
                     /gene="hadB"
                     /locus_tag="KRH39_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBG8510261.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(15327..16127)
                     /gene="hadI"
                     /locus_tag="KRH39_000332"
     CDS             complement(15327..16127)
                     /gene="hadI"
                     /locus_tag="KRH39_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBG8510262.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(16166..17365)
                     /gene="hadA"
                     /locus_tag="KRH39_000333"
     CDS             complement(16166..17365)
                     /gene="hadA"
                     /locus_tag="KRH39_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBG8510263.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            18042..19040
                     /locus_tag="KRH39_000334"
     CDS             18042..19040
                     /locus_tag="KRH39_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG8510264.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            19402..19929
                     /locus_tag="KRH39_000335"
     CDS             19402..19929
                     /locus_tag="KRH39_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBG8510265.1"
                     /translation="MEKMNKVTSKKTTYIITSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            19956..20552
                     /locus_tag="KRH39_000336"
     CDS             19956..20552
                     /locus_tag="KRH39_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBG8510266.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFAKDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(20631..22214)
                     /gene="asnB"
                     /locus_tag="KRH39_000337"
     CDS             complement(20631..22214)
                     /gene="asnB"
                     /locus_tag="KRH39_000337"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG8510267.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(22279..22416)
                     /locus_tag="KRH39_000338"
     CDS             complement(22279..22416)
                     /locus_tag="KRH39_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510268.1"
                     /translation="MKNKKFNVITLIILDNHIYAINKEQISTNSKRIYFEKSGFFSDY
                     K"
     gene            complement(22779..23615)
                     /locus_tag="KRH39_000339"
     CDS             complement(22779..23615)
                     /locus_tag="KRH39_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG8510269.1"
                     /translation="MNYIIKKVLNSSVVLVHDANDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(23639..25105)
                     /locus_tag="KRH39_000340"
     CDS             complement(23639..25105)
                     /locus_tag="KRH39_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG8510270.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDVDV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVIDMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLAAQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIAKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRIDYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(25108..26973)
                     /locus_tag="KRH39_000341"
     CDS             complement(25108..26973)
                     /locus_tag="KRH39_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8510271.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILDLEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDINDSTIKTGEKINLGARFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLNCNRLVSIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLAIMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTIIILFLYN
                     KMGFLVVGLLAVVLPFMVATGMHKALIPYAINTIGKLGYEALYMTASLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAILGVVLSSAISGTFLG
                     LFTVKGFVLVGPGLASMSMFIDPNNGNNLIFAIIGFVVAILGSFIITLIIWKEDANTA
                     ESSTEDIVEEELIEEEKEVNILVSPVEGKVIDISKVNDELFASKTLGDGVAIIPANGN
                     LYAPCDSEVVMLFETKHAIGLKTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEG
                     DLLIKFDLDKISHANLDSTVMIVNNNGSDYAYKVLNKSYGNVKKRSILFDVKGGI"
     gene            complement(27220..28083)
                     /locus_tag="KRH39_000342"
     CDS             complement(27220..28083)
                     /locus_tag="KRH39_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG8510272.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(28138..29070)
                     /locus_tag="KRH39_000343"
     CDS             complement(28138..29070)
                     /locus_tag="KRH39_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBG8510273.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGKSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKIGEKLNVPVFKHKILKNPKDLPIW"
     gene            complement(29156..29707)
                     /locus_tag="KRH39_000344"
     CDS             complement(29156..29707)
                     /locus_tag="KRH39_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8510274.1"
                     /translation="MQSLNEIIAENLKKIRKEKRLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            complement(29936..30427)
                     /locus_tag="KRH39_000345"
     CDS             complement(29936..30427)
                     /locus_tag="KRH39_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510275.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(30455..31015)
                     /locus_tag="KRH39_000346"
     CDS             complement(30455..31015)
                     /locus_tag="KRH39_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG8510276.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            complement(31167..32324)
                     /locus_tag="KRH39_000347"
     CDS             complement(31167..32324)
                     /locus_tag="KRH39_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG8510277.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANELLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDNILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(32361..33302)
                     /locus_tag="KRH39_000348"
     CDS             complement(32361..33302)
                     /locus_tag="KRH39_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510278.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDKKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKENNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLGNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNTETNNIN"
     gene            complement(33389..34081)
                     /locus_tag="KRH39_000349"
     CDS             complement(33389..34081)
                     /locus_tag="KRH39_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG8510279.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DVVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(34191..35003)
                     /locus_tag="KRH39_000350"
     CDS             complement(34191..35003)
                     /locus_tag="KRH39_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBG8510280.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(35054..35965)
                     /locus_tag="KRH39_000351"
     CDS             complement(35054..35965)
                     /locus_tag="KRH39_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8510281.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(36024..36755)
                     /locus_tag="KRH39_000352"
     CDS             complement(36024..36755)
                     /locus_tag="KRH39_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBG8510282.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFNKLNDVYKSLPAGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKVLDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSERLSFFDELMTLDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLI"
     gene            36844..37095
                     /locus_tag="KRH39_000353"
     CDS             36844..37095
                     /locus_tag="KRH39_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510283.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGTKFINKKR"
     gene            37329..37748
                     /locus_tag="KRH39_000354"
     CDS             37329..37748
                     /locus_tag="KRH39_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510284.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKAIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLNSPINLKSGDIFTFIRLTLLAGRMF"
     gene            37773..42830
                     /locus_tag="KRH39_000355"
     CDS             37773..42830
                     /locus_tag="KRH39_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBG8510285.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALKLIFTSEQVEELCQLIKR
                     KIDYTYSGGYYRKGYRSNKLVDYFRGIIRFISSYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEDVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     CCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFNARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     GDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     TSSLIKSSNSQKCLGGLEIVNLLKDNTDRQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSHSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENANYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(43015..43752)
                     /locus_tag="KRH39_000356"
     CDS             complement(43015..43752)
                     /locus_tag="KRH39_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8510286.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(43771..44706)
                     /locus_tag="KRH39_000357"
     CDS             complement(43771..44706)
                     /locus_tag="KRH39_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG8510287.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            44914..47490
                     /locus_tag="KRH39_000358"
     CDS             44914..47490
                     /locus_tag="KRH39_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG8510288.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YTNKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            47502..48173
                     /locus_tag="KRH39_000359"
     CDS             47502..48173
                     /locus_tag="KRH39_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510289.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            48200..48541
                     /locus_tag="KRH39_000360"
     CDS             48200..48541
                     /locus_tag="KRH39_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510290.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(48901..51543)
                     /gene="adhE"
                     /locus_tag="KRH39_000361"
                     /gene_synonym="adhC"
     CDS             complement(48901..51543)
                     /gene="adhE"
                     /locus_tag="KRH39_000361"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG8510291.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT        17369 a         8780 c         6187 g        19773 t
ORIGIN      
        1 taatttaagt aatagtatat gtaatcttta gtcatcttct taccagtttt ttatccactt
       61 ttctaatgaa gttatttttt catatgttaa ttcctcttga aataatgata ctaaaagact
      121 ttttataaaa ttataactta aaagtttatg tatttttttg atatgaattt atagtactta
      181 tcttctttaa ttgagattgt gtatatttaa ctaagcatta caacaataat tccatgcaat
      241 caaatcaatt atttacatat ttttttagaa caataataag tatatattca caaaattttg
      301 tgttaatatt tttaatttgt gatataataa aagcaaggaa acatatttta cttaactttg
      361 agtgatgttt ccttcaacaa attattttga agatttactt ttctggaact ttatattaag
      421 ttcccagcca ctcttagttg gtcgctcaga gtggctcttt aatttatcgt aaatgtaact
      481 ggctataaca ccagttataa tactcatcat tagattttcc atagttttca cctcctctca
      541 taagaaagtg aagaaacttc catggaaaca ccactcatag ctttttcaaa tatttattcc
      601 ttgccatatt aattatatca taaatattgg aaacataaaa ttttaatact tatcgtcttt
      661 attattattt tttataatta ataattttct ttttaataat acataatata aatttatatt
      721 tcttcaataa aaaagacaat cttaattaat tgtctttttt aatatatatt tggctaatac
      781 attattttac acaaacatta caagggtcta ttttaccttg tgacttagct tgttttaatg
      841 ttactttatt tattgtttta cttcttttta gtgtagaaca attttttgtt agatgatatg
      901 attttccatt aggagttaca tatactgtac ttttatcagt taatccagga ttattagagc
      961 taggctcatt acttggtttg ttcgtattat ttgaattagt atcttttaca atacctgtat
     1021 ttgaatcccc aacattatta gttatattta aacattgttt tataacaaca tcatcaatat
     1081 ttccaatagc agttatttta cttgcatttt ttaatatact cttattactt ccatttccaa
     1141 ctaacactat aggtgttttt tgtgcaattg tagaaccaac taaagaataa actaaatcag
     1201 ttccacttgc tatgtaaaat tcttttgagc cattataaaa tttatttatt atttgtttat
     1261 ttgtatcgta tctatctact ccacctattc ttgttgcttt agtttcattt attaacttat
     1321 cactcatact gacttttcca ccaattgcat aagtttctaa tccatctgta acaaaacttg
     1381 tagtatttcc atcagttaat actattggag atttatctct aactgctata gaagcaacac
     1441 tcatagcatc tggttcgcct ttaaaagcat tagttaatat tactttttta accgtactta
     1501 ctcttcctat ttcttttgca actttatgac ttgtatctat tctatcagtt ccacttattc
     1561 tatttatgct tattttttta tcacttaata ttttctgaac tgatgctcct atcgaatttt
     1621 ttcctccaat tagatatact tcttttacct tttctaatct ttttaatgtt tctgaaggga
     1681 ttgtatcttt cttagttaac aatattgggg catttttagc tcctgacaat ccacttgctg
     1741 acaatccatc agataatcca ttttcagaat taataagtat tgcaatatca taattttgtt
     1801 tatctgctat taaacctgct gtttcatact tatttgtacc ttttattgaa ttaatagaac
     1861 taagagcgtt tgatgttgga caactagcaa tcaataatga aacagacatc cctattgcca
     1921 atagtgtttt gcttaatttc ataatgaaat cctcctataa attttataat attacttaaa
     1981 tattataatt ctacataaaa taatattttc ctttatgaaa ttagtttata tttcaaaatt
     2041 ttgtcacttt ttaaatctat atactaaaaa atagactatt tgtaacagtc tatttcttag
     2101 tatatcaaat atttatttag gtttacgttt ctttgtacta gtttttgtat atgatattcc
     2161 agttccaggt atactcaatg tagctgattt tcttcctttt gaattaactg acactctagc
     2221 acctttaaca ccagcactta tacctacact cttcttacct aaatttaatt tgagtcctcc
     2281 tcctagattt atgcttttcc taaagttaaa tcccatagat ataaccttcc cttctatatt
     2341 ttaaatattt atacttaaaa ttatatttgt tatacacttg tcatcattgg tatatctagc
     2401 atctatatct ttttacgttt tttcttctta tatactactc cagcaactac agctgctgtt
     2461 ccagctccaa ccccataagt agcatttcta cttttctctt cttctctttt ttctaaacct
     2521 tcatcatata caccttttaa cttagtttca atagacttca tttcatctgg tatactaaaa
     2581 tcttcttcat tgtaaccttg attatataca ctttcttttg ctttaggtaa cttaacctta
     2641 ccattatcat atccttcttt aaaccaatta gcatatttaa cattacctat agttgatgtg
     2701 tcaaatcttt ctcctttgat agcactctca aatccttgag tagtgtaatc attttttaat
     2761 tgttcttgta tttcgtcata agcattatta taagcatcta taaattcaga atctatgttt
     2821 tcttcaaatg tcattttttc tttatcttta ttgctatcct ctatcccttt agctttatat
     2881 tcttctatct ttttagtctt atattcattg tatccagttg aatatccggt atcataagca
     2941 ttttttaaaa cttctttttc atatgtatta ttagatttag cacctgtaag ccctaaatta
     3001 tatccagtct ctttagccac cttttcttcc tcttctaatt tagccttacc tttttcatat
     3061 ccttctgagt actttgattc atatgtatca ctataatctc cactataatt tgatgaacta
     3121 taatttcctt tatacccatc ttcataccct tgattatatc ctttttctcc aacagacctc
     3181 ttttgctttt cttcattatt aaccgaagtt gttgttccag aactgcttcc tcctccagta
     3241 taaggacaac caccattatt atgtaaatga gctggataac caccacaatg atagtgataa
     3301 cttcctaatc cagatttatt tttattatca tgatgacctc ctgatgaatc agttcttcct
     3361 gaatgagcat acgaaaagct ccctacacta atcatagtta tactcattgc aacagctatt
     3421 aactttttct taaacataat ctatttcccc ctaaaatatt ctatgatttt attatatcag
     3481 tcaatatatt atgtttaaac cgttaaaatt cgacaatgta aatatttgat gaattgtatt
     3541 tgtaaataaa tcttttagca tatattgaat atctgtagtt gttttaacat catattcttc
     3601 aataagtttt tttaatattt cgtttttgtt aaacataaaa agcgtgcact tttctttgta
     3661 tagtgatatt tgatatttta attatcacta ctttagaaga atacacactt tattttaaaa
     3721 accctgatac tatgttatta gaaaatggta ctaatataaa agatatccag catatactcg
     3781 gacatgctaa aacttcaaca actatggata catatttaca tgtaactaat aacatgagaa
     3841 accagacggt aaatattttt gaaatcattg tagaataaat ttgccaccca aaaataatta
     3901 tggtggcaaa taggtggcaa atctactttt aagacacaat ataaaagtct gaaaccctgt
     3961 tattctctaa taagtttaac tacagtctca caatgtggcg tatttgggaa catatccata
     4021 cacttaacca tcttaatctc ataaccatac cccttaaaat caaccaaatc aacaaccaaa
     4081 gacttaggat tacaagaaat ataaacaatc tccttagcac caaatccaca aatatcccta
     4141 atagcatcct tatgaatgcc tggtctagga gggtcaacaa taatcaaatc aggcttgtcc
     4201 tttaaatcct taacaacctt agccacatcc ccagcaataa actcacaatt agtaagtcca
     4261 ttcaacttag cgttctcatt agcagcaaca acagcttcct caactatctc aataccatac
     4321 actttcttag cagcaccagc cataacctgt ccaatagacc cagtaccact atacaagtca
     4381 aatacaactt tatcattgta atctccaata aaatccctag caatactata aagaacctct
     4441 gctcctttag tattagtctg gaaaaatgaa aatggagaaa tcttaaactt aagacctaaa
     4501 atttcttctt gtatataatc tcttccatac aatactctta actcatcaca ttggaccgca
     4561 tctgctaaac catcattaat agtatgaagt acccccacca actcagattt taaatcaatc
     4621 cctaaaagca tatctttaaa ctcattcata tcaaaatctt cttgagaaga agttactata
     4681 ttaaccataa tctcattagt atgaatccct tttctaacta caagatgtct caaatatcct
     4741 ttgtgattca ttgttctata ataaggaagc tttttttcat tgaaaaactc tacactagaa
     4801 actaatatct ttgagaagtc ttcatcaacc aacatacact cctcaacagt ttgtatatct
     4861 atatgctttc cttttttatg aagacctaat gtcaaaggtc cattttttac ttcatctcca
     4921 aaagtatatt ccatcttatt tctataatat ttgttttctg gacttccttc tatccctaaa
     4981 aactgaaatc caaataaatc ttgctttaaa aataaatcca taacttgttt ttcttttatt
     5041 tctaattgtt tttcatatgg cacagacaat agtgtacatc ctccacactc tctaaagtgc
     5101 ttgcaagttt cttcagtttc aagtggtgag ttctctaata attctatcat cttaacttca
     5161 gctttcttac ttctaacttt tttaactcca gctctcacct tttgaccact tattccaccc
     5221 ttcatatgga taactctatc tccaacttgg cttaaagatg taccaccaaa ttccatttta
     5281 tctacttcaa attctataac atcttttttc ttcacagaca ttcctccaat ttactatagt
     5341 atctttatta tctttcttca atcaatttta cttcttcatc tgttaactct ctatactcac
     5401 ctaactctaa actttcatcc aattttaaat tgcccataga caatcttttt aagtatacta
     5461 cttttttgcc tacgctttca aacattcttt ttacttggtg aaacctacct tcatgtattg
     5521 taagctctat ttctgattca tcatcacttt ttaatatatt taactgtgat ggcatagttt
     5581 tatatccgtc atcaagcact acaccttcta aaaaagcttc tacatcttct tcagtaacta
     5641 ctccatcaat ttttgcataa taagtcttag gcacatgctt cttaggagat aaaactctat
     5701 gagaaagctt tccatcatta gttaaaacta ataatccctc tgtgtcttta tctaaccttc
     5761 caacaggaaa tggttcaaaa gctaagtatg ataaatcaat taaatctaat actgttgggt
     5821 catatttatc tgtagttgct gatatatatc catcaggttt attcatcatc aaatatatat
     5881 attctctata tataacttct tctccattaa ataatatctc atcattttca gtgtctactt
     5941 gagttcctgg atttgaaact tcacttccat ttactactac agacccttgt ttacaatact
     6001 tctttatttc acttctgctt ccataaccta aatttgaaag aattttatct atacgttgct
     6061 ttttagccat taaaaatgct ctccattctc ataatatttt tctttttcaa atactgtaaa
     6121 tgctaaatcc atactttcta catctaaatt atttcttata ctatcagtta ttattcttgc
     6181 tactatatct tgtacttctt gtcctctatc aaaccaagct acttcaacaa aaggatatac
     6241 atctgctatt ttaccatctc ttattgcaac tgactttata cattctattt caaagtagtc
     6301 tcttggacat tttacagctt caactaaatc atttatcatt ttttcactta ttttacatat
     6361 atcattttca ttaattcctc ttatttttat ctgtggcata gtttatctcc tttattttaa
     6421 atttttagtg aattatcatc cacttaattc ccttcgataa cgtttgaagt ggtaacttct
     6481 tggtcaaggc aactattgtt atcagtatct ccaaactaat aaggtagttc ctacccttat
     6541 agttagcatt atatagctaa ttttcctaaa ttgtttatat tctttattat tttatttaat
     6601 cctattgtct tctatttaac attatacttg taatttatag ttattacaat ttactatata
     6661 ctttttttga tttaacgtta taatataagt gatgggtaaa ttttaaaagt taataggagg
     6721 agtttaaaat ggcattaatt actacaaaag aaatgtttaa aaaagcttac gaaggtggat
     6781 ttgctatagg tgcatttaac ataagtgact tagaacaatt acaaggtgtt ttaaaagctg
     6841 ctaaagctaa aaattcttat gttatgatac aagcttctat gtcagctgtt aaatatgctg
     6901 gacctcatac attagttgaa atggttaagg ctgcttctga tgaaataggt gtagatgtag
     6961 ctcttcactt agaccatggt ccaaatatgg atgctattaa aacttgtatt gatgctggtt
     7021 tctcttcagt tatgatagat ggttctcatt ttgactttga agaaaatgtt agaataacta
     7081 aagaagctgt tgaatatgca cactctaaag gtgttgttgt tgaagcagaa cttggtgttc
     7141 tagctggaac agaagatgat gttacttctg atgttcacaa gtatactcaa ccagctgagg
     7201 cagttgagtt cgttgaaaga acaggtgttg attcattagc aatagctatt ggaacttctc
     7261 atggtgcttt caaattcaaa ggagaagcta aattaagatt cgatatacta gaagaaatcc
     7321 aaagcaaatt accaggtttc ccaatagttc ttcatggtgc atctgctgtt gaccaaaacg
     7381 ctgttgctac atgtaatgaa tttggtggaa atatagctgg tgctaaaggt gtacctgtag
     7441 atatgttaag aaaagcatct tctatggctg tttgtaaaat aaacatggat actgacttaa
     7501 gactagctat gactgctgct atcagaaaat tcttagctga aaatccaaaa gaatttgacc
     7561 caagaaaata cattggtgct ggtagagatg ctatacaagc tgtagttgaa agcaagatag
     7621 atgatgtttt aggttctgct aactctataa actaatataa cttttaaata tttataaaag
     7681 aaaagagatg acttaaagta attaaattta tttttaagcc atctctttta agttgtatca
     7741 attatgtatc ttataatatt ttaattatat tgtcttttta aactgacagc taaacgtgaa
     7801 tatgtccact ctttatcttt ctatggatgg tagaaggata tatacctata acttcagcag
     7861 cctttgttat tgaaccatac ttatttatag ccttacttat cattatttgg tccactattt
     7921 tatatgcatc attaagattc ataattccat ttaccactat tggtgataaa tcatctgttt
     7981 cattatcaat catatctaaa tttataagca tgttaaattc atcttctcca attacatttt
     8041 tagctgataa tactacaaat ctttcaatga tgttttttaa ttctcttatg tttcctggcc
     8101 aagaatattc ttctaataac tcaataactt taggcgaaac ttctacttct ctattgtact
     8161 tttcattgta aagttttaag aaataattta ctaatggtac tatatcctct tttctttctc
     8221 taagaggagg tatttttact ggaataacac ttaatctata gtacaaatct tgtctaaatt
     8281 taaggttatt tctaaggtct tcatgagaaa tattagtagc acttatatat ctaacatcta
     8341 tttttatagg cttactacca cctattctag taatagtatt ttcttgtata accctaagta
     8401 attttttttg catctgcatt ggtaactcac ctatctcatc caagaaaata gttccaccat
     8461 tcgcctcttc aaaaagtccc tttttacctt ttttcttagc tccagtaaaa gaaccttctt
     8521 catatccaaa aaactctgac tctaataatt catttggtat agctccacag tttatagcta
     8581 taaaaggttt gtctctcctt gaactatact tatatattgt tcttgctatt atttcttttc
     8641 ccacacctga ttctccattt ataaatatag aagaatctga ggctgcagct ctcttaacaa
     8701 ctttttttat attgtccata atcttacttt gacttatata ctctgtgtct tctgatatat
     8761 cactatattt tatctgagat tcaagcttaa taacccttga aatatctctc cctgttagta
     8821 ctcctcctgt aagctttccc ttagaatccc atcttggaac tgccgtatat gcaataagct
     8881 tcccatctgg gtaaacgata tttctataaa tctttttctt cactttaagc atgtttaata
     8941 ttattggttc ttcaactaat ccttgtctca caaggtctgt tatatttcta ccaagtacct
     9001 cagttctttt aaaaggtaat atctcatcac tgacagtgtt catcttttca attaccccat
     9061 ctttgttaaa cacaacaaca acttcatcta tatgctctat tatatcttca attactttat
     9121 cagatattag agacttctca aataaataaa ctatattatt ttcattatta ttatctttca
     9181 tattgtatat atgtgcagtc atccttaact ctctgaaagc tattttcttt ttactatctg
     9241 tcagtatttc tgttttgtca aaatttgaat ctaattcatt tatatttcta ggctttttta
     9301 tatctagaaa actaaaaata ctatctatta gatgattaca gtatattatt tcgcctgatt
     9361 ctttacataa aatggctgga ttttctacat gttctataag ccctattaaa ttattatcca
     9421 ttaatattcg cacctttcat taagtaattt attgaactac atacaaaatt tattgtttgc
     9481 ttacaataaa taccatcagc acatataatc aactccttat aatattcgtc ttttcaagta
     9541 gtttatttca ttaaaacgta actaagatat aattttaatg attatagttt gttaaaaatt
     9601 tcattttgct tttaattgta ttttatcaca tatttagtaa ttatttgtct atttaataca
     9661 atttaattaa atttaagatt tatttaaaaa aacaaagata atccttacag aatataactc
     9721 acaatttgta agcaatatac atataaggat tatccagatt atcttgtatt ataaaatttt
     9781 attcaaacag actttttaat aactgacaaa tacacactag caataactta tcaaagggtt
     9841 aataattatg ttactaacta ataagactta ttcaatcagt aatatttata tagtttgtat
     9901 ctattaagct tttatagctt ctaataaatc atcaacgcta tctaattttt ctataagcat
     9961 tggaactatt tcatgtaagt ctccaactac tccatagtca gcgatttcta aaattggagc
    10021 tgctgggttt ttgtttatag caattatgaa atctgatgat tgcatacctg ctaaatgttg
    10081 tattgctcct gatataccac aagctatata taggtttggt ttaacagtag ttcctgtttg
    10141 acctacttgg tgagaatggt ctatccatcc agcatcaaca gccgcacgag aagaaccaac
    10201 tactccacct aatttgtcag ctaatttctt aagcatttca aatccttctg gtccacctaa
    10261 tcctaaacca cctgatacta taacatttgc atctgtaaga gatactaaat ctttcttagt
    10321 tttaactgtt tctaaaacag tagttcttat atcatcttga gttattttgt agtctaatgc
    10381 tataacttca ccagttcttg tttcgtcttt ttcagcttta tccataacac caggtctaac
    10441 tgtagacatt tgaggtctat ggtttggaca aatgattgta gccattatgt ttccaccaaa
    10501 tgctggacga gtttgtttta ttttcttatc ttctgggtct atttctaact tagtacagtc
    10561 agctgttaat ccagttccaa ctcttgaagc tattctaggt gctaagtctc tacctatatg
    10621 agttgctccg aaaagcatta tttctggttt tatttcatct attgcatctt ttattacttt
    10681 tgtatatgca tcagttgtat atttttctaa aagagcatca tctgcaacat aaactttgtc
    10741 agctccaaaa gtgattaatt ctttagctaa tccatcaaca tcttttccta ataatattgc
    10801 acaaagttct gcatctactt ctttagctaa ttttcttcct tctcctaata attctataac
    10861 tactggagct atttttcctt ctctttgttc tgcaaatatc catacgtttt tataagaact
    10921 taaatctttt atatcattca tagtttgtac tcctccaact tatataatgt gcttttgttt
    10981 tagaccgatt attattttac tagctatttc atcagcttta ccttctaaga tttctccttt
    11041 tcctcttggt tcaggagtaa atgatctaaa tactttagtt ggtgatgctt ttaaaccaac
    11101 ttcttcttta tttacatcta aatcatcaat tgtccaaact tttacatcat gtttatatgc
    11161 ttttactatt ttatcaacag acatgtatct aggtgtattt aactctttta ctgctgttaa
    11221 aagtacaggt gtacttactt ttattaattc gtatccatct tctaattgtc tttgaactat
    11281 tatatctttt ccttctattt tgaaatcttg tacatatgta acttgaggaa tgtctaattt
    11341 ctctgcaatt tgtggtccaa cttgtgcagt atctccatct attgcttgtc ttcctgcaaa
    11401 tattatgtca tagtcaccta ctttagatat tcccgctgca atagtatttg atgtagccca
    11461 agtatcagct cctccaaatg ctctatcact taataatatt gcatcatcag cacccatagc
    11521 taaacactct cttagcataa agtctgcttg aggaggtccc attgttataa ctgttatagt
    11581 tacatcatca tgctcatctt taatttttaa agcttcctct aatgcatttg catcatctgg
    11641 gtttaatata cttggaactc catctcttat aagagtacct gtttctttat ttattcttac
    11701 ttcgttagta tctggaactt gttttacgca aactaatatt ttcaaagtct atacctccaa
    11761 tttcattaga ttatttatta ttttaatacg tttgatgaaa ttaccatttt ttgaacttct
    11821 gatgttcctt cgtatattga tacgattcta gcatctctgt acattctttc gatttcataa
    11881 tcttttataa atccatatcc accgtgaagt tgtaatgctt tataagcaac ttcatttgca
    11941 atttctgatg catagtattt agccatagca gattcttgag caactctcat tcctgcatct
    12001 ctcttttgtg ctgcatcata aactaatcct ctagctgcac aaacttttgt ttccatgtca
    12061 gctatagtaa attgagtatt ttggaattta gctataggct taccaaattg aactctttgt
    12121 tttgtatatt taacagcttc atctaaagca ccttgagcta ttcctagagc taaggcagca
    12181 acacctattc taccaacttc tagagtaccc attgctatct taaatccttg accttctttt
    12241 ccaagtaagt tttcttttgg aactttaacg ttttcaaata ttaagtcaga agtttcagtt
    12301 cctcttatac ccatcttatc ttcatgagct cctgttgaga aaccatccca tttactttct
    12361 actataaacg ctgatgttcc tctaagtcct ttacttctat ctgtaactgc tattactata
    12421 gctacatcac agaatggtcc attagttatg aaagtttttc taccatttaa tatatagtgg
    12481 tctccaacta attctgctgt tgtttgttgt ccacctgcat cagaaccagc tcctggctca
    12541 gttaaaccaa atgctcctat taattcacct gttgctatac cttttaagta tttttgtttt
    12601 tgctcttcag taccattgta aagtattggt aatgaagcaa gagaagaacc agctgtaaca
    12661 aaagtagctg ttgaagcaca tcttctagct atttcttcca ttactatagc gtaagaaaca
    12721 tatcctgctc ccccaccacc atattcttct gggatattac tgcttataaa ttttgcatca
    12781 gctaattttt tgattaattc cttaggcatt acacctgttt tatccatttc tgctggtaaa
    12841 gtatctaatt ctttactaac aaaatctctt actgcttttc ttaaaagttc ttgttctttg
    12901 ttatataaca tagcttcctc caattttcat attttatagt aaatttattt taatgaactt
    12961 acgctaaact catcatctca gcaaaagttt gaatacgagt tctggcttgt tcattattct
    13021 gagtttgttg gtcgatttca acatataaag taggtattcc actatcttct atatcttttc
    13081 taactaaagg ataatcgtat tcttctgggt cacagaattt catcatacag aagataacac
    13141 catctatatc tttcttttta acttcatcta ctataagtga cccacgtttt ttcttagggt
    13201 cataagctaa tgaacatcct tctatgtttg accattgtct tgctaatctc tctaacgcat
    13261 catcacctgc tggtacatct gttctgaatt gtcttgtttc ttgtgctaag tcgtcagcta
    13321 caactgatat attgttgtct tctaaaatgt ctaatatatc tttagaatca gctaatatac
    13381 ctgttaataa aactttcttt ccagaacaga cttcttctgg catagcattt aattttgcta
    13441 ttaaatcttt cactagctca gtatgttctt cttttctcat aaagaaccca ctcttaatta
    13501 ctaagcttct tattgatggt tttatagtat tagaatgttt ataagctact tcaacaaagt
    13561 ctctcatagt ttttctatgt tcattgtaaa cttctatact ttcatgaatt tttgcttctt
    13621 ctatttcata tccacaaatt tcttcaagtt ctctttttac gcctttgtac tcactgatta
    13681 agtattttac accagcttca agttttctat tttgtgggtg tactaatgat atatatttta
    13741 tatgaggtac tgctgatttc cagttttgtc ctaaacaaat tagtgtatca cacatacctg
    13801 gtataataac tccagataat tcatcataag caccttttaa tccatattct aaacatgatt
    13861 gcattattga acatgcaaat gcagggaaat attgtttagc taaatctaat tctgtttgtc
    13921 ctccccatat accaactgga agcattccag ctgcatgtat aatttcttct gggcaataaa
    13981 ctgggaaaca acctatagct tttttaccag tttcactttt atattttttt acagccgcat
    14041 ttggattttc aactacttct ttcattttag ataaaatagc ttccattata tctcacctct
    14101 attaagtttt tttctttctt ccattacttc aactaaacct tgaattcttg tttcaaattg
    14161 agcattagtg aaagctctag ggtctgcttg gtcaccatca aatccagcat atggtaatcc
    14221 agtttcttca gctgctcttc tttgcatttc atattgtatt aaactcataa gtttacagct
    14281 tctgttcata tgatagaatg ctccatcaca tttaccctct actaaagaat caactctata
    14341 ttttgtcata cgatctaggt ttacattgtt aaacatagta ctatatgcta ctgccattcc
    14401 atctaaatca ttaacttcat attgtaatgc ccaagcatgt gggtaaacac tacctgtcat
    14461 gttaactcca aatttagcta atgttttcat cttgtatcct atatatggcc aacaaggtat
    14521 accttccatc attattctgt atttttcttc ccctctgaaa gaagatttac cagttttcat
    14581 gttgtcctct aattcttcta taagtaactt aaatgcttct gtagtttctt ttttacctct
    14641 agcacaaact attacagcca tgtaagtaaa taagtcaaat ccattcattg gagaaggaga
    14701 agaatctgct ggtaaactca tagaatactt ccatagtctt ccgttttcag ctgatatttt
    14761 cattacttct tcaaacttct tagggtcaaa tttctttcct gatataattt ctagttgttt
    14821 tatagcttct tcaaattgag ctttaatata atctattctt gattgagtaa cttcgtcttc
    14881 attattgaaa gttgtatcaa tcattattaa aggtatatct aattctcttg aaatattttc
    14941 ataccatttt ataacttggt tacatatatt gttacagcaa agtaggaaat ctggagctgg
    15001 catatccaaa gcttcacatc caccattttc taaaagacca aaatttgttc ttgcatatgc
    15061 acataggtca atagaatatc ctttagattc agctatttca cataattcta aagaaccttt
    15121 tttagctgct actccagctg cttggttttc tggatataat acgtttaagt caaatacttc
    15181 tgctaactct tgaggaaata ctgatgttga ccaacctaca ggtcttcctt cttctttagc
    15241 tttaaatgca tttgcatatt gttcagctaa taaatcatta attactactc tagcttcttt
    15301 tttttcagac attttcatcc ctctttttat atatttttca cttctttttg tgattctttt
    15361 gcttcatcaa aagcatataa cgctgctcct aaagcaccag ttaattgagg tatatcaggt
    15421 acaataattt ctgtgttgat ttctcttgcc atagctctta caataccact atttctagca
    15481 actccaccaa ccataactac atttctttgt actcctattc tttttactag gctagaaact
    15541 ctctttgcta ctgaagtatg aatacctgcc actatatctt caatttttgc attttcagat
    15601 aaatgtgata taacctcaga ctctgcaaat actgtacatg tactgcttat tgatacttca
    15661 ttttgagaat tcatagatat acttccgagt tcagatacat caacctctat tatttttgcc
    15721 attacatcta aaaatcttcc tgtacctgca gcacatttgt cattcataag aaagtttaat
    15781 agtcttccat tattatctaa ttttaatacc tttgcatctt gaccacctat gtcaataatg
    15841 gttcttgtct ctggaattat gaaatttacc cctctagcat gacagcttaa ttcacttatt
    15901 tgcttatcag catctgaata gttcattctt ccatatcctg taactacaac ttttttaata
    15961 tcttctcttg caagacctgt cttgccatat aatttttcta aaactcttga tggtccagta
    16021 gtcccagtac cagaggatat tgtttcagaa gctacaatat cttccccatt ctttaagatt
    16081 actccctttg atgcagttga acctatatct aatcccattg tgtacatctt tagcattctc
    16141 cttttaaaaa atctagaatt actaattaat atcttacaac tttactatct ttaaagttat
    16201 ttattttctc ttcatcgtag cctaaagatt ttaatacttc tacagtatgt tgacctactt
    16261 ttggtgctgg tgtatattct ttaattcctt catttctaaa cataactgga gtattaacta
    16321 agacacctgt atttccgcta tcgtatgttt tcttaaataa gaagtcattt gcccaagctt
    16381 gttcgtcatc taataaatct tcacagcttt gaattttttc aaatggtaag tctgcttctt
    16441 ctaataaagc tgaccactcg tctaatgttt tttctaacat agcttctcca actatcttaa
    16501 ctaaatcttc aacatgatta accattgaat ctatgttatt atatctatcg tcttctaata
    16561 tatattctct atttataacc ttacagaatt tgcctaacca cttgttgtat tgtattaaag
    16621 ctaattgaat ccatcttcca tctttacatt tatatgtagt cattaatggg ctgtttggat
    16681 tttctcttga taaaggcatt tcatttccgt attgtgctgt tgttatcatt gttcccattc
    16741 catatatagc tgtatggaaa agacttactg ttactctctc acctttacca gtttgagctt
    16801 ttttatgtaa tgctgctaaa cttcctgctg ctagtgctag acctgcatag tggtcaccaa
    16861 atcctgctgc tgtatttgct ggagatgttc ctttttccat aacagattgg ctaactcctc
    16921 ctcttgcgaa gtatgcagta tagtcaaatc ctggtttatc ttttaaaggt cctttttcac
    16981 catatcctaa tatttgagag aatattaatc ctggatactt atcttttatt tggtcataag
    17041 ctatacccat tttttctaat gcttgaactc taacattagt tacaaatatg tctgcttctg
    17101 ataataattt atgtaatatt tctactcctt cttttgattt tacattaata cttacaccct
    17161 ttttatttcc attttctaat tcaaacatag ggttttcatc atctgatgct ggagatttaa
    17221 atgttccacc cataactctt attccatcac cttctatagg ttcaatctta ataacctctg
    17281 caccccagtc acctaacatt tttgcacaac atggtgctgc gatgaaactt gaaagttcta
    17341 ctactttaac tccttctaaa agcataacgt tttcctcctt aatagtatca attataaaat
    17401 ttcgattaaa tcactcataa aatacttttt tctgatatta actgtctgat gtcaaagttc
    17461 ctaatcttat ctaaaataac ttaaaaatta ttaaaattat tttttattgt tttattgtct
    17521 tacacctata ttgttaagca tttatcgtgc caactttata tttaatcaaa atatcgcttg
    17581 atttgaatgt tttttaacaa tatgcaattg aataatcttt ttcatatcgt ggcatcttgc
    17641 cactatccat tgcattttgc tattctttct atgcttataa aagttttttt tatatatttt
    17701 tttatgaaaa agcttgacat ttcaaaaaaa gttttcattg ctaaactcaa aaacacatgt
    17761 tatcattgta attaatcagc aagttagtta actaatttca aacttagata attttttaat
    17821 aatatttaat ctaaaaaata acaatttctt tgataaattt tagagtaatt attacattaa
    17881 ataaaaaatt actaaatatc aaaataacac agggaaaaca atttcaaaac taaattttta
    17941 accttaaaaa ttttgtataa aaaatataac cttatatgct acatattatt tctaaatgaa
    18001 aaattaccat aataatttaa taattacagg agggtattga tatgaaaata ctagtatttg
    18061 gagcacgcga ttatgaagaa ccagtaataa aaaaatggtc tgaagaacat aaggatgttc
    18121 aagtggatat ttatcctgaa aacatgactg aagaaaatgt agttaaagct aaagggtatg
    18181 atggtatatc tatacaacaa actaactata tagataatcc ttatatttat gaaactttaa
    18241 aagatgctgg ggttaaagtt atagcttcaa gaactgcagg ggttgacatg atacattttg
    18301 atttagttaa tgaaaatgga cttatcgtta caaacgttcc ttcttattca cctaatgcaa
    18361 tagctgaatt agctgttact caagctatga accttttaag aaagactcct ctagtaaaga
    18421 aaaaagtctg tgaaggtgat taccgttgga tagctgaact tcttggaaca gaagttagat
    18481 ctattacagt tggtgttata ggtacaggaa aaataggtgc tacttctgca aaattattca
    18541 aaggcctagg agctaatgta attgcatttg accaatatcc aaatagtgat ttaaacgata
    18601 tattaactta caaagattct ttagaagacc ttctaaaaga agctgacctt ataacattac
    18661 atactccttt acttgaagga acaaaacata tgataaataa agatactcta gctataatga
    18721 aggatggagc ttacatagta aatactggcc gtggtggttt aattaataca ggggatttaa
    18781 tagaagcact agagtcagga aaaattagag ctgctgccct tgatacattt gaaactgaag
    18841 gattgttctt aaacaaaaaa atgaatcctg gagaattaac tgacccagaa ataaataaac
    18901 ttctttctat ggaacaagtt atattcactc atcaccttgg tttcttcact agtacagcga
    18961 ttgaaaatat agtttattct agtttaagta gtgctgtaga agttataaaa acaggaactg
    19021 ctactaatag agtaaattag tattttataa atatattttt tcattaatta gctattatct
    19081 aacaaaatag cctttaattt cccaaatata agagagtcag ctcagtatat attgagtaga
    19141 ctctcttata ttatttttta tattaaactt gtactcctct tattcatacc aaattaaatt
    19201 tatattaact tattctaact actcctactc tatacatttt acctattttt acttttctaa
    19261 ttatatacat ttgaatgggt acaatctgta ttttacaaaa aaaacaccta acaaactatg
    19321 aaaattgtat gattaatatt aatatattta taagaaaaca taacaattat gttagctctg
    19381 gatttttagg gagggagtat attggaaaaa atgaataaag ttacttctaa aaaaactacc
    19441 tatattatta cttcagcatt atttgcatcc ataatatgcc tgacaatagc atatatatta
    19501 catattccag ttggtggaaa caatggatat gttcatatag gagatgcttt tatttatcta
    19561 gctgcaacta ttttgccaac aaactatgct atagctgctt ctgcaattgg tgctggtcta
    19621 gctgatttat ctacaggtgc tgctatctgg gtaataccaa ctataatcat aaaaccaata
    19681 cttgtattat ttttcacttc taaaagtgac aagataataa ataagagaaa tatagttgct
    19741 tcggttgtag caggtatagt aggattagtt ttatacatgt ttgctgaagg tataattatt
    19801 ggaagtttta caagtgcatt tgtaatgagc ttacttggct tattacagcc aataggaagt
    19861 tttattgttt ttataatctt aggtatggca cttgataaac ttgattttaa aaaacgttat
    19921 tttaattaag aatttaaatt tatggaggga aaattatgaa tatcttatat acaattgaaa
    19981 atagtatcta tgtaaatata actaatacat gtccatgtag ttgtgttttt tgtataagaa
    20041 acgaaaaaga tgaagttgca aatagtggta gtttatggtt ggagcatgag ccaagtgtag
    20101 atgaagtaaa agaagccttc aataaataca atttagacga ctatgatgaa atcgtatttt
    20161 gtggatatgg cgaaccatta atgagaataa atgaattgat agaggtagca aagtttataa
    20221 aagaaaaaag tagcataaag ataagaataa atacaaatgg tctaagtgat ttaatacata
    20281 acaaaaaaac agctattctg ttaaaagatg ttatagatgc agtatcaata agtcttaatg
    20341 ccccaaataa agaagcttat aatagagtta ctcaaccaaa gtttggagaa aaatcatttg
    20401 attatatgtt agattttgca aaagattgca aaaaatatat aaaagaagta gctttttcag
    20461 tagtagatga aatatctcca gaagaaatag aagaatctaa acaactggca aaaaaacttg
    20521 atataccatt aagggttaga cataagaatt agcaagtaaa attataaaac attaattata
    20581 aaacattaag aatcacctga atggtctttc aggtgattct tcctcttgta ttaacatttt
    20641 acaaaataga tttcatacca cactaggaat gtgtaaatca tccaaatctt tctccaatta
    20701 tcagagtttc cactaatgta gtcattaaaa atctcattaa tctcatctat gttaaagaat
    20761 ttatctgcta tctcactatt gaatttatct cggatgtcct tattatattg gtcatcagcc
    20821 atccatatac gaattggcac aataaatcct aatttatttc taaaagcaat ctcttctggt
    20881 aatgtttttg atgcagctgt acgaaaggca atcttattct cttttccttt cactttatat
    20941 tctgatggta ttctggatgc aatatcaaat attcttgtat ctgtaagagg cattctgact
    21001 tccaatccaa ctgcagtact cattttatct atattaaaat aaatatctcc ttccaaccat
    21061 atttgaatat ctacatttga catgttagta agtgtatcta gttcttctgt ttcttcataa
    21121 attcctttta ccacttcaat aggaagcaca tcaggattgt atttcttaag aatttttctt
    21181 ttctcaccct cattcataat gtttgtattt ccaatataaa atgtcttcaa attctctcca
    21241 taacactctg ccttatggta tatattatat ccaccaaaaa actcatctgc tccttctcca
    21301 gagtaacaga tatttgtgtt ttcagctgct gccttgcatc caatagcaaa aacgatagca
    21361 gatgcatctc cagttggttg ctccatatgg tacattacat aaggtactga cgcaaaatac
    21421 tcttcaggtg taattatata tctcgaattt ttacacccaa gaatattagc tgtctgttgt
    21481 gctaattttg attcatcaaa acgctcttcc tcataaccac atgaacctgt tttttctata
    21541 tctgacattg ccaatatata agatgaatca acacctcctg ataggaataa ctctgcagtt
    21601 tcattctctg attttacctc tggcataatt ttctgaagtg ttgtatgaat ttcatccgtc
    21661 cattcctcaa tagacttgct gtggtcagga tgaaattcag gtttccaata acgaccaata
    21721 catactgttt tattctgata gacaaggtaa tgtcctggca taagcttctt tactccacgg
    21781 aaaaatgtat tttcacctgc tacatatgtg aaagacatat aaatctgtaa catgtcttca
    21841 tttaactctt tcacaaatcc tggttgctcc ataatcttac gaatagatga accatagaga
    21901 agcttcccat tttcagtctc ataataatag aaaggtttta ctccaaactg gtctcttagg
    21961 cagaagagtt tcttttccac agtatcccat aatgcaaaag caaacattcc atgcatgtga
    22021 tttgccatat catatcccca tgtcttatat gcttccacta aaattgctcg ttcacgctca
    22081 tctctacata atgaagaatc gattccaagt tcttcacaga gaactttcca gtttcgaata
    22141 tgtccattat attctagaat ttttatcata tatgtgtctt ttcgtgttaa atacttttct
    22201 tctaaattta tcatatgtgt atcctttcgt attaaaacat ttaacttcta aatttattaa
    22261 atgtttatat atatttattc acttataatc actgaaaaat ccactttttt caaagtaaat
    22321 tctttttgaa ttagtactaa tctgctcttt attaatggca taaatatgat tatcaagtat
    22381 tatcagtgtt ataacattga attttttatt cttcataaat attgccccct ttatattacc
    22441 aactaaatat aatatatctt atgttactta catattcttt aagaaattct gaagatttta
    22501 taaagttttt tgtattataa ttaattaaaa ataaaattct agttataaag catattttct
    22561 attaaacaaa aaatagttaa aataaattca taaccacata aacttatttt aactatttgt
    22621 atattttaat tataaatatt aacttaaatt tttaacagga tacactcata aaataaacat
    22681 aatgaacaac aaaataatat gtctcagttt ctttatatac tttaccttct ttcaactttc
    22741 taccatgtag acgtataaga taatattagt aattcactct attgcttaac tcgtgcgata
    22801 tggacaacca agaaagctat ttcctcattt gttaactttt ttccatattt cttttctaaa
    22861 aaatctctaa cttttaatgc aattaacatt tcttttggat ttgacctttt catttgatta
    22921 aataataaat catcaccact gcttaacata ctatctgaaa aaaatctttc cacaaaaaat
    22981 tttatatgtg ttataaatct catataatgc atactctttt tatctaaggt tctattcaaa
    23041 gaaaaaacaa aaatattaat tatttctcca attactttgg catacttaat cgtatcatat
    23101 gaatttccat ctgaagccat agcatttgca aaatggaaag caatgttagc tgcttcttcc
    23161 tctggtaatt gaatgcccaa tctctcttct accatataaa ttgcaaccat tccaacctta
    23221 aactcatttg gataataatt ttttatttcc caaaatactc gatttgtaat ttttattcct
    23281 ttacgcatcc tttcaattgc aaagttaaaa tggtctgcta atattaagta taagttttta
    23341 ttaaaagatg agttcaataa cttttctgcc tctactatta tttcctgtgt tatctttaaa
    23401 atgtcgacag gaatatcatc cattaattct aaaaattgtt ttgattttgt attttcaaca
    23461 gggacaaaca tttgattatc attggaacca caaatatatt ctcctgtttt tttaccatat
    23521 ccaattcctt tccctagcaa aataaattca ttatcatttg catcatgaac taaaacaacg
    23581 ctcgagttca aaactttctt tattatgtag ttcattttat ttgctcacct tctattggtc
    23641 atttaaaatc tatactatta ttttcaatta cctttttata ccaataaaaa ctatctttct
    23701 tatatcgatt atatgtcccc tttccataat catcagcatc cacataaata tatccataac
    23761 gttttgacat ttgtttcgtt gattcactta ccaaatcaat acaaccccat gaagtgtatc
    23821 cccaaagttc aacaccatct tcaataatcg catctaacat acatttaaag tgttctttta
    23881 aataatcaat tctatattgg tcatgaactt ttttatcttc agttaaaata tctttagctc
    23941 ctaatccatt ttcaactata aataaaggct ttctatatct atcatataat tcaatcaaag
    24001 atatacgtag acctattgaa tcaatttgcc aaccccattc actcatttct aaataaggat
    24061 tttttacagc taataacgta ttcccaggtg taatatctaa tccagttgta tcagctgctt
    24121 cacaagaaga catatagtaa gaaaatgaaa taaaatctac tggatacttt ttcataattt
    24181 catcatcatg ttcttctttt gctatttgaa tgttattatt tttaaaatac gacagtaaat
    24241 atactggata ctcaccaaaa acctgtgtat cactatatgc ataaatactt ctcatttttt
    24301 gttgagctgc caaaacatct tctggtctac atgtatatgg ataatatgtt aattttgtta
    24361 acatacaccc tacttttgaa tttggaataa tctcatggca aatttttgtt gcaagggcac
    24421 tagctacaaa ttgatgatgc atagcttgaa aaatgacttc ctcaaagttt ttatccttaa
    24481 aacggtctcg aaccaaacca ccagtagtgt atggatgacg aatcatagaa tcaacttcat
    24541 taaatgtaag ccaatatttc actttgtttt tatatctttc acagatagtt tctacatacc
    24601 taacaaacat atctataact tgtcgtgaat accatccatc ataatttaaa acaatattta
    24661 atggtggttc ataatgtgac atagtaacaa gtggttcaat tccataactt attaactcat
    24721 caaatacatc atcataaaac tgtaaacctt cttcatttgg ttctttatca tctccatttg
    24781 gaaaaatacg agaccaagca atactcattc gatatgtctt aaatcccatt tctgccatta
    24841 gagcaatatc ttctttatag tgatgataaa aatcactacc atgacgtttt ggataattta
    24901 cttcatcttc aggatgtgct aatccttctt ctatatcttt tgttgtgatt tcattattag
    24961 atttatagtc tttcacatct acatcaaagt gtgcctttgc tacatctgat acagaccacc
    25021 ctttaccacc taaattccaa ccaccttcaa attgattagc agctgtagct cctccccaaa
    25081 gaaatccttc tggaaacttt ctcatattta tattcctccc tttacatcaa acaagatact
    25141 tctttttttt acattgccat aagacttgtt taacacttta taagcataat ctgacccatt
    25201 attatttaca atcataacag ttgaatctaa attagcatgt gagattttgt ctaaatcaaa
    25261 cttaattaac aaatctcctt cttttacatt atcgcctgtc ttaacaaatg ttttaaatcc
    25321 atctccattc atacttacag tatttattcc aatatgaatc aaaatctcag ccccattttt
    25381 tgtttttaaa ccaattgcat gttttgtttc aaatagcatc acaacttctg aatcacaagg
    25441 tgcatataaa tttccattgg ctggtataat tgcaactcca tctcccaaag tcttacttgc
    25501 aaataattca tcattaacct tacttatatc tattactttt ccttctactg gagatactaa
    25561 gatatttact tctttttctt cttctattaa ttcttcttca acaatatctt cagttgatga
    25621 ctctgctgta tttgcatctt ccttccagat aatcagtgta ataataaatg aacctaatat
    25681 tgcaacaaca aaacctataa ttgcaaaaat taaattgttt ccattatttg ggtcaataaa
    25741 catgctcata ctggcaagcc ctggtccaac aagtacaaat ccttttactg taaataatcc
    25801 taaaaatgta ccagaaatcg cacttgacaa tacaacacct agtattgctc ttttatgttg
    25861 taaagtaata ccatataaag ctggctctgt aattcccatc aaagcagaaa ttcctgcaga
    25921 taaggctgtt tgttttaatg tttcatcttt tgtacgtaag gctactgcaa aacaagcacc
    25981 actttcagaa atattgtgag ctaatgaagc tgtcatatat aatgcttcat aacccaactt
    26041 tccaattgta ttaattgcat aaggtattaa tgctttgtgc attcctgttg caaccataaa
    26101 cggaagaaca acagcaagta atcctacaac taagaatccc attttgttat ataagaataa
    26161 aataatgatt gtaaaaattg taccaacgtt atatcctaaa ggacctaaaa tagttagagt
    26221 aattggaaca gtaattgcca aagacatcat aggaacaaag aaagttcgaa ttggttttgg
    26281 tgaatacttg ttaaaaattt tctccattat tgccaagaaa gacacacata agatagctgg
    26341 gaaaacctgt gcattatagg caatgtttgg aattgtaaat ccaaatagtt cgacaccctc
    26401 acttaaagca gttgttgttg ctggaagcaa taagtatcct acagcggcaa tagatactaa
    26461 acgattacag ttcaatacat tggcagtagt tacagctacc ataagaggta ggaaatagaa
    26521 tgttgcatct gaaatagcaa ctaataatgt atatatagta gaatctgatt ttaaaatatt
    26581 aaacattgct aataacatca acagtgattt taaaatacca gctcctgcaa tagcaaaaat
    26641 caatggttga aaaatattaa taataaattc tataaatctt gctcctaagt ttattttttc
    26701 tccagttttt atagtactat catttatatc taaaggacaa acttttaaaa tttcatcata
    26761 aacatcaaca acactatttc caatgacaac ttgaaattgt gccccattaa ctatactcat
    26821 aacaccttgt acttttttta aagcatcctg attgacttta cttctatcaa tcaacttaaa
    26881 gcgtagtcta gttgaacagt gttctaaatc taaaatattt tttgaaccac caacttgttc
    26941 aataatatca atggcagttt ttttataatt catcttgtaa tctccttctc cataattttt
    27001 tatgaatggc cattcaagat gcaaaaaagg cagaacctat ataaacacct tgaaaaatca
    27061 aagcaatata ttcgatcctg cctgcattac cagtaacatg tcttctattt atactatttt
    27121 gtattattat attaacattt gcctaaaaga attgtcaata cttttttaaa ttttctgata
    27181 ctaactaata gcacctttat aattcatttt ttatatcaat taaagtaaca gtaaccaccc
    27241 accaattagt atcaaaattg aacctggtat tttttctttt atattttctt tgaatataaa
    27301 atgagcaagt attaaagtta ttatcataga tacttgagaa aatggctctg ctatactaag
    27361 ctctagatta tttaaagcaa ttaataatgc taaataagaa tatccattaa tagcaccact
    27421 taaaattgaa agcccctttt tattactaaa aatctcacaa attatttttt gtttcttttt
    27481 aaatgctaaa aagataaata aatttaaacc tacgaaaaaa tacagtatag ttgaatataa
    27541 tataggagat acttgtacaa aaaaagcctt gtccaatact cttcctactg actgcaaaaa
    27601 tatgtataaa aacattaatc tacatggtaa attattaaaa attacagaga cattctttaa
    27661 aaaagagctt acttttttta atataaacac tccaccaatc ataattatga ttcctgcaac
    27721 ttttgctaaa cttaaacctt cacctaaaaa tataactgat aaaagcagta aaaacaatga
    27781 atttaaacta tttattggag taacaagtga aacttcccca gttgctaagg acgttacata
    27841 cgctactgaa ccaattgaat agactaaact acttatataa catggtatta aaaacgacca
    27901 atcactaact ctatccatta tactaaatgg aagtaaacat actccaccta taaagaaaaa
    27961 tagaaatgat gaagctatat ttttataaat atctccttct tcatcaccta ataatcttac
    28021 aactactctt tcaaaagcta atagcactac tctaattaat aatgcaaaat aagataatat
    28081 catttttgac ctcgcttaaa aattaatccg acttattgtt atattaactg tataagacta
    28141 ccatataggt aagtcttttg gattttttaa tattttatgt ttaaatacag gtacattaag
    28201 tttttcacct attttacgac attgttcttt ataggtacct tttaatttat catgaaaaac
    28261 tatacaatca attccacctt caatactatt tatacacttt tctagtttct caaatcccga
    28321 ttcatcatca tacattccac ttacaatcca ttgatttggc aatacatgtc caattgatat
    28381 gttatattct ttatttttta taatatctga gaaagatttc tcaaaatgta cccctccaac
    28441 acataataaa gtcttcaaag gttcatcaca atcaaatact ctaattaatg attttgccaa
    28501 tacctgtatt gctatactat tattaaaact atcgctacta cttcctatct caatatccaa
    28561 cataggtaca gaatattctg taattaattt agattcttca ccatatgttg ttccagacca
    28621 gtgagttgct tctgttaatg tagtaaattc atcaagtaga ttttcatttt ttatatcctc
    28681 aatagcattg attagatttt taaagtatct tggatttgac tttccaaatt ctcctgttgg
    28741 aacatctcca attgtatgag ctgttaggat atgttctgga gcattttctc cctcatgcca
    28801 atttacaact cctgcaaaat cataatctct gaaatgtcta tttaaaattg gaagataata
    28861 tttataatta tgacttatta catcttttgt ttttacaaaa tgaaataagt tattgttttt
    28921 atccttaaac tctaatacaa aattatcatc aataacaata tcagtttgat ttaaatccat
    28981 tatttcttca caacacttta tcacattact ggctactggg tctttcttta aatcactaca
    29041 aaagaaaaaa actgcttttt tattattcat ccataaatca cactcctata acttaaatat
    29101 aatatatctt catacctaaa atcatctaaa tatcactcta gttccattaa attatttata
    29161 tataatatac taccatactc attttagcta tacctttgcc tgagtttcta tatatatgtg
    29221 atacatcagc tttaaatctt atagaatttc cactagatac aatgtaatct tctccattta
    29281 ttttaatggt tacttcgcca ctaaaaacag ttataaactc ttgtgttcct ggaagttcag
    29341 catttgaatc taaatttcca ccttcttcta tttcaatcat aaatatctca aatcttcttt
    29401 tggagtcaaa tggaaatatt ggataactta tatactttcc attatcctca ataaaatggt
    29461 ctatatcact tttttgaatc aattctatgt cactttcctg cctctccatt aatgatgtaa
    29521 atgagatttt tagtccatta gttattttct ttaaagtaga tatagtagga tttacctcac
    29581 acttttcaat ttgagatagc atactcttac ttacaccact aagttgtgca atcttgtcta
    29641 aactaagacg tttttctttc cttatctttt ttaaattttc agcaattatt tcatttaaac
    29701 tttgcataaa taccccctaa tacatactaa tataagataa attcttttga ttattaaata
    29761 aacttagtaa tttaatagaa actttttatc cattataatg tacattatgt ttaatataca
    29821 ttactttttt tagaatgtaa atagttttct gaaattttgt gaaaatgaac aaataaaaaa
    29881 ctagtaacct atatttttac aaggctacta gcttttatta taataatatt tcattttaac
    29941 tataaaactt agatgcacct ataagctgtc tcttagaaac aatcttagct aggtttgcta
    30001 atgaagaaat ttttatatct ttaaaagaaa ctaaaaatac aaacttaaaa tctgtaaatt
    30061 cttgatttat attgtatata tcaaaataac atctcaaatt atcaaataag taattatctt
    30121 gtaagtctgc ttctatctca tcagtagcat cttttaaata cgctcttaca tcttcaaata
    30181 aaacactgaa ataagactca tctatttctt cacctaactc ttccaatatt tcttctatat
    30241 ctaaatcatc catatcaaaa gagtttcctg tcaactggca atcaaatacc aatatatttt
    30301 cttgatttat agtatttgat atatcttgta catattcgaa gtctggacgt aattctaaaa
    30361 tgtcttttat ttcactgctc tttacatgga tttttccttt tctattttca atttttaaat
    30421 ctatcatatg ctcccccttt caatctacaa atctttaaat accttgtttt ataatctttt
    30481 caatatctat ccaattacta actctaacta tattttgatg tttaacatct ttattatagt
    30541 tagtatccag aagtattact ttaacaccat catcagctag ttgcactgag tttaaagggt
    30601 tatcttctat aaatatgtca caactcagtt ctcttgcctt ttcaacttta taatcacttc
    30661 ctaataagta aacatctata tctgaaaatc cttgtctcga tagccaattt ttagttacat
    30721 cagttaaaca ctcatgtcta gccgttacaa aagataaatt atgtccttta gataatgtgc
    30781 taattacctc tgtagcaccc tctacaatct ttgcttcatc atatgagtgt atatacttat
    30841 tattaaactc cgcatacaca tctcttattc ccgaaccgta taattcttcc caattatgtg
    30901 ttatacactc tgcttcagtt atatttttat taaagatatc atttaggtat ggaagaaaat
    30961 gataagggct tgttattgtt ccatctatat caatacatat attcaatttg ttcattttta
    31021 cctccatgta acatttattg atttgattaa tcaaatatta tagcacagct ttaattataa
    31081 atcaaaactc tagatatgac tatacctata taataccaaa aagcttgcat atttaagcaa
    31141 gcttttcgta aattttacat ttttaactaa tatataagtt tttctaaggt tggttccact
    31201 ccaaattgtg atttcagttc tttatattct tttaatgctt tgtcattatt cgttttagtt
    31261 tttatcgctc ttatcattaa attttttggt gtatcaagtg gagatatgta ttctactaca
    31321 gaagtatcat atccattgcc ctccaataac aatgttctta aaccatcagt tattaaatct
    31381 gcaaatctag ctttaaatac accatgtttt aatattggtt ccatagcttc atatctatat
    31441 tgccctaata actccttatg acaacatggt actactacta tagcttcaga ttttgctcta
    31501 attcctagac ctattgccat atctgtagct gtatcacaag catgtagact tattacaagg
    31561 tcaacatccc tattaggtgt ataattagtc aaatcctctt ttataaatga catattttta
    31621 taacccaagt ttttagccat attttttgat gcttctataa ctacatctga ataatctatg
    31681 cctataaaat aacagttttt ctttaatact tcttttatat aaaaatttaa gacaaagctt
    31741 aagtaagatt ttccacaagc acaatccaag atagttatac agtctttatc ctttatttct
    31801 tttaaaatat tatctatcaa ttcaacaaaa tggtctatct gattgtattt tcttatttta
    31861 tcatttttta tcttaccatc ttttgtaagt actccaattt cttttagaag ttcatttgct
    31921 tgtcctactt ttacataata atctctgttt tttatttgag atgccgtaaa ttcatctatt
    31981 ttaggtactt cttccttatt atctaaatat tttacattta ccttcttatt gtctccttca
    32041 attacaacaa tagttcctcg ctcaacatat ttaaatacta atccatcata attttctgcc
    32101 tctttagaaa tattcttaag caattcatta aacgtatata aatctgattt tcctttaaaa
    32161 ttaaatttat atttattatc ttcctctatt gcaattcctt taaactcttt tcttccagat
    32221 ttaaaaatta cctctaactc aataaaaaaa tccctattat caaggtttct ttgagtaatc
    32281 cccataaaaa acatatttag ttttcctata ctctgtttat tcatcataat ctccctttta
    32341 acttatatcc ttaaatctta ttaattgata ttatttgttt ctgtattgtt tatttcttct
    32401 tttaaatcgt tcaatctatt tttaaaactt tctatctgct tatctgataa atcaagatat
    32461 tctgttatct tccctatata cttagggctt ttagtattta ttaatttcaa agcactttcc
    32521 atagattttt ctctaagctc atatgctttc tttacatcat ctctagcatt acccaatgtt
    32581 tctgtatact tctccttaga aagttctgga atctgcatat tttcatattc ttttacaact
    32641 tctttacact tgtttcttgc atttgtaaca tcttttttta tactcgataa tttagttata
    32701 ttttttgcta tatccttagt gtaaggtata cattctctca ttggaactaa agctgctctt
    32761 tctatattat aaatttcttt cttaaaaatt cctatacttt ccatatctat tttttttata
    32821 tcttcactat ttttttcttt acttgtatat ttattatttt cttttgtatt atcttcttgt
    32881 ttttctgatt tattattcac tgaaacatta ctatcagaat tattattttc ttttatatca
    32941 ttacttcctt cttcattact ttctttattt tgtatatttc tatcttgttt gttgtttaaa
    33001 tttttattat ttgatatttc ttcattcttt ttcttatcct gttttaaatc agtactttct
    33061 ttttccttaa aatttgatac atacttttca tcatatgtct catcagagat ataattgtga
    33121 taatacatag actcttttat tttattaagt ccactcatat aaatattact tatcattatt
    33181 gttgtagcta ataccacata aatactactg tagatacaag acctcttaaa attcttgtaa
    33241 tttctgaata tcactactaa aggaataaga tataaaaata taaaaactaa tatccacctc
    33301 ataagtcgtg tctcctttct caaaaccgaa atccctcaaa tatttatttt taatcatgta
    33361 tcttatagta tttaccaatt tatgtttttt aatcgtgaac ttttattaaa ttaaattctg
    33421 tatagtcaca agatgctaat gtatactcta ctccatttct aatccccttc aatcccattt
    33481 taaaagattc tttaccatgc aagtgaccat aaatcacctt ttcaacatta tattcttcaa
    33541 ataactttgt aaataaagat tcttctaatt tatcattcgt aggaggataa tgagtaatca
    33601 caattatttt tgagtgacca cttttacgag cagattctaa tgataaccta agtctatgtt
    33661 cttctctttt atacactttt tcatcagtct catcaaattt aacatcatta ggacatatcc
    33721 agcccctacc tccacaaatt gcataatctt tatattcata aaaatttgtc tgaataaaac
    33781 gcatgtcttc atatagctta tttaaactcg taacggttgt ccaccagtaa tcatgatttc
    33841 cttttataag tattttctga cctggaagtt tgcttattat atctaaatct tttttagctt
    33901 ccgataaatt aattccccaa gatgtatctc ctagtactaa gaccacgtct tcttccttaa
    33961 caacttcttt ccaattatct ataattttct tttcatgacc atcccaatta cttccaaaga
    34021 tattcattgg cttattgact gatgttgaaa aatgtaaatc acctatagca tataaactca
    34081 taatatcatt cctttcttga ttacactaaa taggctgtct ctttttagaa attattttcc
    34141 attaagaggc agccccatta atatttagca aattttctac aagtagtttt ttactgaact
    34201 actttatcat ctaccttagt atccttatat tgctctagta atatatctat ttcttgtttt
    34261 aattccaaaa tttcagttat atttttatct gtttcagttc tatcaagttt tctttgatac
    34321 tgcatagctt tatgtgtatc acccaagtca tataactctt gtattctatt tagagtatct
    34381 ctaatagcta tagacttcat ttggtacata gcctgagctt tcattatgtc ctctcttatt
    34441 tcactcattt cttggtctaa aagaaaggca gcatcagatg ctcttctaag tctttcagca
    34501 agagtctcag gtatgtaatg gtcatattta tattttaatg aatgttctat tgtagcccag
    34561 aaattcattg caagagttct tatttgtatt tcacatataa tttcctttga acccgcaatg
    34621 gagtttatag ggtattttat tataacatga tagcttctat aaccactatc tttgtaattt
    34681 gtaatataat ctttttctcc tattatagtc atatcatttc ttacttttat caaatcaact
    34741 atagcatata tatcctcaac aaactggcac attattctta taccagcaat atcatcaatt
    34801 tcagtttcta tatcttttgc atttatcctt tttaacttag atattatgga agatattttt
    34861 tttgttctgc ctgtaacaaa ctctatggga gaatattcac cttttgctaa atattctttt
    34921 cttatatttt taaattttat tttcaattcc tctactgcat gttcataagg tgctagtatc
    34981 tcattccatt tttcgtattc catattgctc accctttatt tgttttttat gaccttcttt
    35041 attttatcat aatttatttt gtttttgtat agtaaagcaa ttctccattt tccaatatat
    35101 accctatttc ttctaaatca actaagtaac ttatgacaga tactaacgaa gatttaaaaa
    35161 aatgatactc tttataatta ttgcttaaat tattgttatt tataatgttt tttaataaaa
    35221 tttctaaaga tttcggttct acaagcaact ctcttatttg atataaatat ttttttaaag
    35281 cattcaaatg tttttcgatt aatttataag aatcttcttt tgatataact tgcttgccat
    35341 gccctaaaac taagtactca aaatctatat tttttaattt ttctaatgaa tttatttgtt
    35401 cttgtatatc atatagaaac aagaaatcgt attttgatag aatttcatct cctatcaata
    35461 aatcaccaac aaaaatcaca ttatccttcg ttaaaacagc tatactacca ggtgtatgac
    35521 cctggaactc aattatatca aacatttcat tgtttaattc tatttttcca gcttctatag
    35581 atttatctat ttttatatca tccaacattt tattttttaa gatattatcc ataaatacat
    35641 tgctctttcc cccaacaata tactttgaaa acaattctgg attttctata tataatttat
    35701 cgtattcaga tgacattata cacacatctt tataatactc tttaaattga ttacaagctc
    35761 catagtggtc atcatgacca tgagtattta taatatatct aacctgtata ttattttcat
    35821 ctaatacttt atttactctc ctaggtcttg aaccacataa ccctgggtct atcataagtg
    35881 ctgtgttatc ttcaaataca tatattcctg tattcgtgcc accttttata aaaaaggtat
    35941 ttccttttac ttttgtaaga ttcaccttct tcacctcatt ttgacaatat ttatgataca
    36001 atttattact tactaatatg aaactatata agaacaatcc tttttattct atctatagtt
    36061 ctttttctta ttttgtattc tttagatatc cttattttca cattatatgc taaatttcta
    36121 taaagtaatg actctataaa atattcaact atacctctat ccctaaagtc tatatctata
    36181 tttccacttg aataaatatt tgagtctaat gtcataagtt cgtcaaaaaa acttaatctc
    36241 tcagatttgc ttaaataatc tttatttgga ataaatctat cacaataatc tatcctgtaa
    36301 tttactactt catcatttat ttcaaatcca tattgctttt tcattaactc tctatagtta
    36361 ttatttttct caataacatt atctaagttt ttattataat cctctttttt ccaatgtcca
    36421 aacatcctac aatttaatgg tctaacttca tatattaaac atctattatt agtatcttta
    36481 aatggacatg ggttctttct tgtatactcc tcaaaataat aatctaatac tttacttata
    36541 cagctttttt ttaaatcaac tctatcttca agatagcaaa atatattcaa aaactcaatt
    36601 aaatttatac ctactgactc catacaacaa tttccacatc cagaacaatt tcctgctggc
    36661 aaggacttat atacatcatt tagtttgtta aagagcttat ttttaatgga ataatctata
    36721 caatttaaaa tatctttatt ttttatacta gtcattatta ttactccttg ttataattat
    36781 taagttaata caaacaatta ttataacata ttttttaaat ttaatcgcat atggaggtat
    36841 tttatgaata gatatacaaa aataattaat atgatgggaa gttattacac aaaagacttt
    36901 gaaaaagaaa aaaagaatgt aataaaagtt agagaagtta aagaagatac agttagaaaa
    36961 tttttcttac aaggtgactg tgaagttctt gtagtatttg aagacacagg gaaagaaata
    37021 cttattgatg acttttctcc tgaagaagat atcaaaaaat atttaggaac aaaattcata
    37081 aataaaaaaa gatagttatc aaaaataatt ctaaaattaa aatttaataa atgtacagtt
    37141 ataattctat ataattatag ttaaaagtta tcttttattg cttttttaga ctttaagata
    37201 gctttttttc tattttttgt aaaataattc cataattcac attaataatt cacaaaatga
    37261 tataatataa caaatagata aatttttaaa tttctaaata cataattaat attttgcgag
    37321 gtgattttat gcaaatttat tttagagtta aggctgttgg caaacgcaaa cctatgctag
    37381 aacttacccc ttttgatgta tctgacaaca tcaattgcct aaaagaagct atctctgaaa
    37441 ttgtatctaa aaatgttctt gaatataatg agaaaatccc agaaaaagca ataataaact
    37501 tcttaactaa tgaagaaatt gaatctcaag ctgatattgg aaaagttgga tttggttcta
    37561 tttacaatga gaataaacaa tctgtagaaa aggcaataga ggtagcgtta caggcatttg
    37621 aagatggaat ctacaaagtg cttataaatg aagatgttat tgaaaaactc aattccccta
    37681 taaatcttaa aagtggagat atatttactt ttataagact taccttgctt gctggaagaa
    37741 tgttttaaaa taaataattt ggaggtacat aaatgttaga gcgtactaga gtagaaaaaa
    37801 atcctaaaat aattgaaaat tttaaaaata attttactaa atcttcttca aaacagtact
    37861 acgacttagc aaaatctctt tgggactgca tagaaaatag ttcatactat tatagagaac
    37921 ctgaagacaa attcttaaat atgataaaga aatactacaa ttgccactac tacgaaatta
    37981 aaaatatgga cgagctcctt tctggacctt taaaaaatgc attaaaatta atatttactt
    38041 ctgaacaagt agaagaactt tgccaattaa ttaaaagaaa gatagattat acatattcag
    38101 gaggatatta tcgtaaaggg tatcgctcaa ataaacttgt ggattatttt agaggtatta
    38161 taaggtttat ttcaagctat gttgatttaa tttattatga tttttcagtt atagattatc
    38221 ttactcttcc aaataaggtc ttgtactatg actctataat gagtgataaa atagcactag
    38281 aaatagacaa aggaaatcaa aaagtaataa gtgccttaga agacgtaata tttggagaaa
    38341 atcaaactgc tctacttaca agacaaatga taattggtat catgaagagt catagtgata
    38401 aatgctatga aatgctttct aaacttcttt tagcagccaa acaacaagaa ggacttagac
    38461 agcaaattgt agagagttta gatgagggta caatttctgc atcaatatac atgttaaaaa
    38521 ctattttgga caacaattta gagcgattta gttctgttat aagagccttg gatacatgga
    38581 caggacttgg tttttcagac caaaaacctg gaataatacg taaatgttta gaactttcat
    38641 ataaatcatt gactgatgaa aagttccgta tagaatcaat taatagcaat gataatcttg
    38701 aaatttattt tgccttatgg gctactgcaa cttacaacat atatgactct tactctctta
    38761 ttgaaaactt actttgttgc caggaaaaat ataaaaaact tgtagctctt tactttttat
    38821 cgcatacaga aagtacatat tttaaacatt ctatagcatc taaaaacatt aagggtgatg
    38881 atttagagat actagcatgg gtaattacaa atttctatgt agaaggttct gttggctact
    38941 actatagttg gcaaaacaga gaaactaatt ttgacttgtc aaacactcct attgacaaag
    39001 actttaatgc tagaaaagaa caatttgaaa gtttaaagca tactttagaa attttaaatt
    39061 caaagacaaa atcattttca ggaagtgtat ttccatggac ttggattgaa atttcttctc
    39121 atgaaatact taaatgtatg atgactttaa cagcatacga tatgaatgct caatttatag
    39181 atgtgcttat ggaaaattgt catcttatga gttcagattt aagagcttca cttatagtca
    39241 attttgtaaa tgaaccaaag acagatagac aacgtgaatt tttattggaa tgtttaggtg
    39301 ataagagtgt ttccaataga gaaacggtat tgagtctact tagaaagctt gaactaacag
    39361 atgtagaatt agataagata accaatctat tgtctttaaa gacaagctct attagacaaa
    39421 cagtaataaa aattttactt gaacaagatg attcaaattt ggaaaatatt acttcttcac
    39481 tcataaaatc ttcaaattct caaaaatgtc ttggaggtct tgaaattgta aacttattaa
    39541 aagacaatac tgatagacaa gaactatata ataagcttat tttacttata gaagatattg
    39601 ataaatctaa aaagaataca tctcaagttc aaatactaat tgataatttg ttgggtaaag
    39661 caaaaaatga ttactgcaaa gaaaatggtt ttggaatata taaccctgaa aacaaagttc
    39721 aaattccaga acaatttgat tttaaagatg attctcatag ctcatcaaag cttaataaaa
    39781 ttatttcaaa cataagttta tctactcgcg aggattctgt ttctgtagca acaaaaaaag
    39841 aagcaaaaaa aattctcgat acggatataa ataaaataat aaaagtttat gaaaaatttg
    39901 atgagcttat tactgaaaat gcaaattatg agtatgaagt tgaaagatgg tcaggtaatg
    39961 tagacaaagt acttttaggt tattctactt ataacttgac tcctattaaa tataaagttt
    40021 ctaatgatac tcttgataat tatccattag cagagctttg gagagaaact agtaaaaaac
    40081 ttgacttaga ttacaaatta atacttgata tcttatttta tagtataagt acatgtggat
    40141 ataatgaaac agaaaaaagt tggttttcat ctatatattc aaatgtcctt gactctaaaa
    40201 aacaaaataa aatatataca aaacttgaaa aacttaaatt taatggactt acaagaaaac
    40261 ttttaagagt tttactaaat gagtttgacc ataaagaaat atttaactat tgtttagaaa
    40321 tatactataa acttagaaac ttattgacta tagaacaatg taaaaaacaa agagttgaat
    40381 ttactaaaaa ctattacaga caaaatgaat actgccctgc tttaaatgcc cctattttaa
    40441 actattggct tgataaagca cattcgttta tttatgatga tatttctttt actaagtttt
    40501 tctacactac gttttcaaat tatcagttgc tagaatataa gtttcctttc tatactagca
    40561 taaatgtgtt tgctcgtgca catgaattag gattaatgac tgaagagcaa gttaatagag
    40621 aaatgataag tggatacaac gcatcaaata taataagaac attgacaaat cctagatggg
    40681 ctggcgacac attagaaaag tacccttggc ttaaagataa tttaaaaact gttacaaacc
    40741 gaattataga aattgaatca cgtagaggtg atatgccaac agaagtgtct aatctagcta
    40801 ataatataca atactttgaa ggtataaatt attttgtaaa tattttggtc gctcttggta
    40861 aagaaacttt ccagagagga tattcatatg gtagcaatta ttcaaaaaaa gatgttttaa
    40921 gttctctact taaaaattgc tatcctacta aagaagatga ttcaaaaaaa ttaaaaagtt
    40981 tacttaaaaa taccgacatt actgagttaa gattgataga atctgctatg tatgcccctc
    41041 aatgggtaaa cataattgaa aaatatttaa actggaaggg tctgaaaagt acaatttggt
    41101 acttccatgc acatataaat gaaggcttct ctgcacaaaa agaatcagag gttgctctat
    41161 tctcccctat ttcaccacgt agtttcattg atggtgcatt tgatgtagat tggtttttag
    41221 attcatataa aactattggg gaagaaagat tcaatatact ttacaagtca gctaaatata
    41281 taactagtgg tggtaactct catagacgtg cacaactcta tgctgatgct acacttggta
    41341 agcttgataa agacatgttg atgaaagaaa ttgaaacaaa tagaaatcaa gataaattga
    41401 gaagtttctc tttacttcct atgaaaaaag gagacctaaa agaagcaact ttccgttatg
    41461 aatttattca aaagtttgct aaagaaagca agcaatttgg tgcacaacgt cgtgaaagtg
    41521 aatcaaaagc ttgtactata gcgctagaaa atctagctag aagtgctgga tttggagatg
    41581 taaatcgtat gatgtgggct cttgagactg aaaaactaaa tgaagttgct catctattta
    41641 aaccaaaaga tattgatggt acttcaattt ggatagatgt tgatgaaaac ggaaaagcat
    41701 ctactaaagt tcaaaaaggc aacaagtctt taaagagtat accaaaagct attgctaaac
    41761 atccatatgt acttgaactt aaatctgtcc aaaaagattt aaagagtcag tattctcgtg
    41821 caaaacagag tttagaacaa gctatgaaaa atgaaatagt atttacagtt aaagaaattg
    41881 ccaatatcta taaaaatcca gttatctgcc ctttgattaa aaatctagta tgggtatgtg
    41941 atgataatat tggatatgtt tataatgata agtctaagtt ctttatttgc aatatagaag
    42001 gtgaaactta taagttgacg gcaaaaaata aattaaaact tgcacatcca gttcatttgt
    42061 ttgaatctgg taagtggagt gagttccaac atgatttatt tagtaagcaa attgttcaac
    42121 catttaagca agtattccgt gagtattata gaataaacag tgatgaatta tctgaaaaaa
    42181 ctatatctcg cagatatgct ggtcaccaaa ttcagccaaa gaaaactgta gcacttctta
    42241 gaaatcagct ttggactgtc gattatgaag agggattaca gaaagtattt tataaagaaa
    42301 atatagtggc aaagatgttt gcacttgcag attggttttc accagcagat atagagcctc
    42361 ctactattga aacagtagaa ttttttgata gaaaaactta taaaagttta gaattagctt
    42421 ctatacctcc aattctattc tcagaagtta tgcgtgatat agaccttgtt gtaagtgtcg
    42481 ctcatgctgg tggtgttgac cctgaaacta gccattctac tgtagagatg cgtatagcaa
    42541 ttgctaaaga acttttatta ttattaaaaa ttaacaatgt caattttgaa ggctctcatg
    42601 ctcttatagc aggtaattta ggtgagtatt ctgtgcatat gggttctggt attgtacata
    42661 aaaaagggat gggtgctttg aatatacttc ctatacattc tcaaagtaga ggtaagatat
    42721 tccttccatt cgctgatgaa gaccctaaaa ctgctgagat tatgtctaag atactattac
    42781 ttgctgaaga tagtaagatt aaagacccta gtatcttaag taatttataa gtataaaaac
    42841 tatctctaaa atatattgga ctttcttaaa aaaattagaa agtccaatat atcctatact
    42901 gtaatttata tagattaaat cataattaca aataattttt atataaactt ctcttataca
    42961 ttatctttac ggtacttatt atatcacaca aaaagtattt ttcttctatc ttcactagaa
    43021 tcttttaacc ctataaaatc tatataaaaa atctttttct agagatttgt ttaatatggc
    43081 attttcttga aaatttataa accttatctc taatgaagat ttatttatag gtataacctt
    43141 atgtataaat cttagattta ctatgtattg tctatgggtc ataaagaaat taggaggtag
    43201 gcgttttttt aaatcatcaa agttctcata aaactttatt atatcttcat ctttagtgtg
    43261 tagattaatt gctcttgaat ttttctcaaa cataactata tcatcaaaat taatcatatg
    43321 tatctgcttc cttattttat atacaaaaag ggtgtcttct ataaaattat tattagcaat
    43381 cttatgggag ttaatatggt ctattgctat gttaattgaa tcaagtattt tttctttttt
    43441 aaatggttta actacaaaat ctataggatg cactttaaag ctttcaagac tataatcagc
    43501 gaaacccgtc acaaatacta tatgtatatc cttatctata gaaagaattt cttcagcaga
    43561 atcaatccca tttttatatg gcatatcaat gtcaataatc aataaatcta ttttctcatt
    43621 ttttataaat tcaattgcct ctataccatc cttaactttt ttaacataat ctacatatcc
    43681 agactcttta gctatatttg ctatatcatc tcttacaatc ttatcatcat cacaaatcaa
    43741 aacttttatc atagactttt ctcctcctaa ctaataatat tttgggatta agattacaaa
    43801 ttcagttaaa aaatcatcac tatttacaag tatactataa tcatacttat ctaatatatt
    43861 tttaactaaa tgtagcccat atcccctatc ttgtccttct ttagtactaa atccattcca
    43921 aaatatactt tcaacattat gtattttagg accattattt tttatagcta taatatactg
    43981 aaattcatct tcatatgcat gtaatcttat atacttcttt tcttttttta tagtagacaa
    44041 agactctatt gcattatcta ttatgtttaa tagtactgaa ctcatatcat taggttcaat
    44101 ctctaaatta tccataaaat cttgaatgta gacatcaaaa gatatatcct tttcaagaca
    44161 ttcctcgtat tttaatgaag ctatagcatc taaataagat acttttccat accttgaatt
    44221 ttttgattta aagctatttg atatattcat tatatattct ttggcatctt catctagccc
    44281 agtatttaat agagaataca ctacttgcaa atgtttcaaa tagtcatgtt tttgacttct
    44341 taatttgata attatattat ccttatgatt tattttacta tctagttttt gtagtttata
    44401 ttcctgttca ttattattat ttatcaatct tataacttta aagcctgtat aaaaacttaa
    44461 aataattaaa accaaaaaaa gtataatatt taatactcct tcaagtaaaa aactatatcc
    44521 taaccttaat tgtatatatt tagtgccctc cataaataaa atcaatgcaa aacacgatat
    44581 aaaaaatgtt aatatgcctc tttttaatat gtctaattct ttgtaaaatt tatacttatt
    44641 cattataaga ttgctaaata taaacaaagt tagtattact ataggaaata caaaattgtt
    44701 atccatgatt cccctcccct ctacaattta tatagggatt atatcatttt aaaaaaatta
    44761 tgtaaaaagt taaaatttac atatatatta attaaacttg taaaaataca gatgaatttg
    44821 tacaattaac tacttaaaat ttgaaaatac ttactttgct atatatttta gataaatata
    44881 taattgattt agttaagaaa ggaagtggta aatatgaaag ataatttgaa aatagtacta
    44941 aggtatataa aaagttataa agctaggtct ttagcaattg ttgtgagtat aattcttgct
    45001 acatcactcg ttgttggtat tggtaccctc tcaagaagtg ctcagcaagc agatgtagat
    45061 agattgaaaa gagagcttgg aactgaccat gtatacttta aaaatataaa gaaaaatcaa
    45121 ttaaagtcca taaaatctag caaagatatt aaaaatctgg gtataacatc ttactatgcg
    45181 tctacagatg ttaatgaaaa attacctata aacatactat atacaaataa aaattatttg
    45241 actagtcatt ctaaacttat aaaaggtcga tttccaaaag ctagcaatga ggtggtagtc
    45301 gagaaatgga ttttaaatag tatgggctta aaaccagaga taaatcaaaa ccttactttt
    45361 aaattatatc aaaaagaaaa tccagaaact tttaaagttg ttggaatcct tgaagataga
    45421 tttatagaga aaaaaaaggg acaatgtgaa attttttcta atctcaatga atcagaactg
    45481 aatgatttta caacttatat tgaatttaat gaaggcagtg atataaatac aaacatagac
    45541 aatataataa aaaatgctat gttagatgag aaaagtatag gaagaaactc aatgcttata
    45601 gaatctacta tgaaaaacgg tactttagat aattcaagta aatacgttgc aattgctatg
    45661 agtctatttg ctggtattgt aatatatagt atctatgtta tatcaatata ccaaagagta
    45721 caggaatatg gcgtcttaag agctattggt tccaccaact ttaggatttt caaacttatg
    45781 ctttatgaac ttttcatact tgctctaata gcaatgccta taggtatatg tataggtatg
    45841 ggaggtgcac aaatatttaa taggtctgta ggaaatattc aatttgaagg aaatataaat
    45901 gttacacctt ttgttattcc agataaaata attctcttat caattgcatg tacaatacta
    45961 ataatactaa ttataagttt ctttacatac ttaaaaataa gaagaatttc tcctattgat
    46021 gctatcagaa aaacttttgg agctgataaa aatgtaacca aagttaatta tttaatatca
    46081 aaattaactt taaatatgtc agtaacaaaa tctatatctg cgaaaaacat ctttagaaat
    46141 aaaaaggggt tcattataat tgtattatct atgagtatag gaggtataat cgtcataaaa
    46201 gacaattata aatattcttt tttagatgtg caaaacaaat ctactcaaga acaaacatat
    46261 atgaatgcag attttattct atcaaatgtt tatctcaatg aaattggtaa caatacttct
    46321 tttaaagata taaaagggtt taatgataat cagatagata aaataaaaaa tatagatgga
    46381 attgacaaag tcaaaactgc tagtatctta aacacaagaa tagaggtaaa taaaataaat
    46441 agattagatt actacgaagc tataaattca actccttatt ataagctaaa tcctctgttc
    46501 ataaaaaata agactactgg caaatataca ctaaagcaaa agctaaaagg ttataatgat
    46561 gagatgataa agtcattaga aaaatactta gtaagtggaa atataaactt gaaaaaaatg
    46621 aaaaaagaaa atttagctat tgtatatgta ccagaaattt ctaaaacaca gaaatacaca
    46681 aagactttta ctcctggtga aggaacacca gctgtagata taaaagtagg tgatactata
    46741 aaagtcaaat atccaaaggg tgggatggat acaggtcttt atgaaagtct aaaagataat
    46801 tatgaatact tagaatatga atttaaagtt ggagctatta taagttatcc ttttgcagat
    46861 aatggtatgt actctggaga tgatggtgta gatgtaataa cgagtagcga atacctaaaa
    46921 aaattaactg gtgtagataa gtataactta gtttatgcag accttaatga aaatgcaaac
    46981 aagaaaaaaa taaataacct tttaggtaaa attggaagtg aagttcctgg aactacaaca
    47041 gtagatatga tggtagaaaa agaaaattct gataagatga catctagaac tatgatttat
    47101 agttatggta tagtagcaat aatgttcata atcagtgtac ttaacataat aaacaacata
    47161 agttataact taacttcaag aactagtgaa tttggaatgt taagagctat tggtatctct
    47221 gagagaggct ttaaaaatat gatactttat gaaggaatat tatatggtgt actctcaagt
    47281 ataattacta tcgtgtcagg aattataata caatttaaaa tgtattatat gcagggcttt
    47341 gtatcatatg gattgggatt ttcaatagat tacaaaatat atatactagt tgttgtagca
    47401 aatataattg taggtattct agctacatat attccatcaa gaaagataaa taaaattagt
    47461 atagttgaag ctataaacat tacagaatag gaggaataat tatgcctata ttggaaacta
    47521 ttaacttagg taagatttat ggaaaaaagg aaacatctgt acatgcacta aagaatgcaa
    47581 atttaaaaat aaataaaggc gaatttgttg ctataattgg accaagtgga agtgggaaaa
    47641 gtacatttct acatttagta ggtggtcttg aaagaccaag taatggtact ataaaggtag
    47701 atggtaaaga tatatgttgt ctttcagata aagaattagc aagatataga agacaaaagg
    47761 taggatttgt ctttcaacag tataacttaa ttcctgtatt aaatgtaaag gaaaatatag
    47821 aattaccact aaagttagac aataaaaaaa tagataaaga atatattgat gaccttataa
    47881 atttattggg tcttaatgaa aggaaaaatc atcttcccaa ccaattatct ggtggtcaac
    47941 agcaaagagt tgctattgca cgtgctttat ctgcaaaacc aagtataatc ctagcagatg
    48001 aaccaacagg aaatttggac agtaaaacta cagaagaagt catggattta ctaaaaagtt
    48061 ctataaaaaa atataaccaa acccttataa taataacaca taatgaaaat attgctagaa
    48121 aagcagatag aattatatct ataatagatg gagagcttaa attaacactc taaaactatt
    48181 tatagaaaag aggatttaaa tgttattgat tttaggtaat ttaaatttaa acttggattt
    48241 tgactacaga ataataagag aagaaaatga tgatgtagat atatttatag atattaatta
    48301 tcgtagctta gatatagata ctgatggttc taacttattt aattctagaa tacaatttcc
    48361 atttgtaaga gcattgattc taaggctaaa taagaacaat caatgtatga caattcattt
    48421 gttaagagat atagatttat tctctgcttt tgccaacttt gaagtggatt atactgactc
    48481 tataataaat ataaaaaatc aaaatgagaa agtaatttta aataaatcta tcaaaaaata
    48541 gtaagggggc cttaataggc cccaattttt ttcttttcta aacttattat atcatgataa
    48601 ataaattagt caatagaatt ttctgaatat ttttataatt ttttcataaa gttttttata
    48661 tattatgctt taaaattata tgtaataaaa aaattttccg actaatttta aagttaattg
    48721 attaaataca catattcgaa attaccatta aattactgac tttttatcat ttccatttag
    48781 aatttaaatt ataatctatt atttatagtt acttctacgt gtctctaata aaactctatt
    48841 tatattcaaa tctaataatt tatatacaaa cccaatagaa cctatgttgc acaatagata
    48901 ttatttacca taataaactt ttagatatat ctcttttaat tcactcatca atgggtatct
    48961 aggattagca cctgtacatt ggtcatcaaa agcttgttca accatagcat ccaatctttc
    49021 taagaattta gattcttcta ctccagcctc ttttatagtc ttaggaagac ctacttttgc
    49081 ctttagttca tctattgctt ttataagatt atctactttt tcttggtctg tacttccttt
    49141 aatacctgca aaagaagcaa attcagcata tctttgtatg caatcaggat atctgtattg
    49201 tgagaatgca cccattttat ttggagcttc tgcacaattg aacttgatta cttcatttat
    49261 taataatgca tttgcaacac cgtgttgaac atggtggaat gcacctaatt tatgtgctaa
    49321 agagtgacat attcctaaga aggcatttgc aaaagacatt cctgccatag tagatgccat
    49381 tgccattttt tctcttgctt ctttatcatt ctttccattt ttatatgcac gaggtaaata
    49441 ttcaaatatt attttacctg cttgtagtgc tagaccgtca gctggttcag ttcttaacat
    49501 agatacatat gcttctaatg catgtgttaa agcatcaaca cctgatgctg ctgttaatct
    49561 tggtggcatc tccatcatca tatcagcatc aattatagcc atgtttggca ttaattcata
    49621 atctgctaat gggtacttta cacctgaatc ttggtcagtt ataactgcaa atggagttac
    49681 ttctgaacca gttcctgctg aagttgggat agcagcaaaa tacgcttttt cacccatctt
    49741 aggaaataca tatactcttt ttcttatatc cataaatctc atagctaagt cttggaaatc
    49801 tacttctgga tgctcataca ttacccacat tatcttacca gcatccattg cacttcctcc
    49861 acctacagct attattaaat ctggttggaa atcagccata gctttagctc cttctcttgc
    49921 acaagctaat gttgggtctg gagctacatc aaagaatgta gtatgcttaa ttcccatctc
    49981 atctaataaa tcagttaatt tcttagtata tccattatta tataggaaag aatctgttac
    50041 tataaatgct ttctttttat ccataacatc cttaaattct cttgctgcta cacctaaaca
    50101 tccttttttg aaataaacct tttctggtgc tctgaaccaa agcatattct ctctcctttc
    50161 agctactgtc ttgatattaa gcaaatgttt tactccaacg ttttcagata ctgagtttcc
    50221 accccaagaa ccacaaccaa gagtaagaga cggtgccaat ttaaagttat ataaatcacc
    50281 tataccacct tgtgatgatg gagtattaac taatactcta caagtcttca tagcatttac
    50341 aaacttttct actctctctg gatgatttat agagtctgca tataatgaag atgtatgacc
    50401 atagcctcca tctgcaacta atttttctgc tttagcaact gcatcttcaa aacttttaga
    50461 cttgtacatt gcaagtacag gagaaagttt ttcatgtgca aaaggttcat ttaattcaac
    50521 agattctact tcaccaatta ttattctagc tgtttttgga acttctacat ccgccatttt
    50581 ggctattaca tgtgctggtt gtccaactat tctggcattt aatgaaccat caattattac
    50641 tacttttcct actttttcag tttcttcttt atttaggaaa tgtgctccac gatatttaaa
    50701 ttctttttta acttcttcat atattttttc tggtactatt acagcttgct cggaagcaca
    50761 tatcatacca ttatcgaatg attttgatac taagattgaa cttacagcca tttttatatc
    50821 tgcactctca tctattattg caggtacatt tccaggccca actccgacag ctggtttacc
    50881 tgaagagtat gcagatttaa ccattccagg tccaccagta gctaatataa ggtctgcctc
    50941 tgccatgaca acattagtta attcaatcga aggaacatct acccaaccta taagtccttt
    51001 tggtgctcct gccttaactg ctgcatcatg tacaactcta gctgcttcta tagtacagtt
    51061 tttagctctt ggatgaggag agaaaattat accatttcta gtttttagtg ctattaggga
    51121 tttgaatatt gcagttgatg taggattagt tgttggtata actgctgata aaacacctac
    51181 tggctcagca attcttgtaa aaccaaatgc ttcatctttt tctatgacac cacaagtttt
    51241 agtatcttta taagcattat agatatattc tgaggcaaaa tggtttttta taaccttatc
    51301 ttcaacaata cccataccag tctcttccac tgccattttt gctagaggta ttctttgttg
    51361 attagctgca gttgaagcag ctaagaaaat cttatctacc tgttcttgcg taaatgttgc
    51421 aaattctttt tgtgccaatc taatttcttc taatctcatt cttaatgttt caacactgtt
    51481 gacaatttca ggactacata cttcaacatt tgatttttct actacctttt cttttttctc
    51541 catactaaaa acctccctaa aataattatg catctctaat ttttataaat cttaaatgca
    51601 tcatctatat ttttaactta tacttaaaat aattttatag atttcatctc tataccttag
    51661 attttattta ttttactctc ttaggtcaag aagttttttt ctgaatattt tgtcaattta
    51721 ttttttaaca aatatattat tttttttact tttactaaaa ttttttatct aaatgagata
    51781 tatttcattt gaataattta cttcgactaa ataaaatact aaaatgataa tttatatctt
    51841 tcattttttt tacttattaa attttatatg ttatttgcat gttatttgta taagtagtaa
    51901 ataaaatttt tgtcgtattt ttataaaata acacttattt ttgacttgta tgcaattttt
    51961 ctttttataa caactgtata gttttaaaaa atatataaac taaaattatt ctaatctcta
    52021 taagttattt ttaagtcaat atgtaataat tatgcttttc atatagataa ataattttta
    52081 tattaagacc gttaattttt taatgataa
//
LOCUS       DAEORZ010000007        50879 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEORZ010000007 DAEORZ010000000
VERSION     DAEORZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.007"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(31..1635)
                     /locus_tag="KRH39_000362"
     CDS             complement(31..1635)
                     /locus_tag="KRH39_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8510292.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            2021..2947
                     /locus_tag="KRH39_000363"
     CDS             2021..2947
                     /locus_tag="KRH39_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG8510293.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(3067..4065)
                     /locus_tag="KRH39_000364"
     CDS             complement(3067..4065)
                     /locus_tag="KRH39_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBG8510294.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            4169..4606
                     /locus_tag="KRH39_000365"
     CDS             4169..4606
                     /locus_tag="KRH39_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8510295.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRLLKKINDNFKNAD"
     gene            4692..5279
                     /locus_tag="KRH39_000366"
     CDS             4692..5279
                     /locus_tag="KRH39_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG8510296.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(5579..6199)
                     /locus_tag="KRH39_000367"
     CDS             complement(5579..6199)
                     /locus_tag="KRH39_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBG8510297.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(6207..6974)
                     /locus_tag="KRH39_000368"
     CDS             complement(6207..6974)
                     /locus_tag="KRH39_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBG8510298.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGAIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(7015..7836)
                     /locus_tag="KRH39_000369"
     CDS             complement(7015..7836)
                     /locus_tag="KRH39_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510299.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     VNIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(8195..10105)
                     /gene="phnW"
                     /locus_tag="KRH39_000370"
     CDS             complement(8195..10105)
                     /gene="phnW"
                     /locus_tag="KRH39_000370"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBG8510300.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     DYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSSISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(10384..11169)
                     /locus_tag="KRH39_000371"
     CDS             complement(10384..11169)
                     /locus_tag="KRH39_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG8510301.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPISIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(11386..11616)
                     /gene="dltC"
                     /locus_tag="KRH39_000372"
     CDS             complement(11386..11616)
                     /gene="dltC"
                     /locus_tag="KRH39_000372"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBG8510302.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(11635..12783)
                     /gene="dltB"
                     /locus_tag="KRH39_000373"
     CDS             complement(11635..12783)
                     /gene="dltB"
                     /locus_tag="KRH39_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBG8510303.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(12783..14297)
                     /locus_tag="KRH39_000374"
     CDS             complement(12783..14297)
                     /locus_tag="KRH39_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBG8510304.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(14418..15608)
                     /gene="dltD"
                     /locus_tag="KRH39_000375"
     CDS             complement(14418..15608)
                     /gene="dltD"
                     /locus_tag="KRH39_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBG8510305.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(15896..16915)
                     /locus_tag="KRH39_000376"
     CDS             complement(15896..16915)
                     /locus_tag="KRH39_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG8510306.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(17091..17630)
                     /locus_tag="KRH39_000377"
     CDS             complement(17091..17630)
                     /locus_tag="KRH39_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG8510307.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(17731..18411)
                     /locus_tag="KRH39_000378"
     CDS             complement(17731..18411)
                     /locus_tag="KRH39_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBG8510308.1"
                     /translation="MQKVEGIIFDMDGVLFDSERIYLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(18440..19729)
                     /locus_tag="KRH39_000379"
     CDS             complement(18440..19729)
                     /locus_tag="KRH39_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG8510309.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            complement(20178..21779)
                     /locus_tag="KRH39_000380"
     CDS             complement(20178..21779)
                     /locus_tag="KRH39_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG8510310.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNIN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     YDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(21890..22567)
                     /locus_tag="KRH39_000381"
     CDS             complement(21890..22567)
                     /locus_tag="KRH39_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510311.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(22580..23275)
                     /locus_tag="KRH39_000382"
     CDS             complement(22580..23275)
                     /locus_tag="KRH39_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG8510312.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQINSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(23327..24526)
                     /locus_tag="KRH39_000383"
     CDS             complement(23327..24526)
                     /locus_tag="KRH39_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG8510313.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(24787..26832)
                     /locus_tag="KRH39_000384"
     CDS             complement(24787..26832)
                     /locus_tag="KRH39_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8510314.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVIRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(27243..28052)
                     /locus_tag="KRH39_000385"
     CDS             complement(27243..28052)
                     /locus_tag="KRH39_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8510315.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(28136..28669)
                     /locus_tag="KRH39_000386"
     CDS             complement(28136..28669)
                     /locus_tag="KRH39_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG8510316.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(28819..30168)
                     /locus_tag="KRH39_000387"
     CDS             complement(28819..30168)
                     /locus_tag="KRH39_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG8510317.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(30180..31562)
                     /locus_tag="KRH39_000388"
     CDS             complement(30180..31562)
                     /locus_tag="KRH39_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG8510318.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSIFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDKPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            31806..33020
                     /locus_tag="KRH39_000389"
     CDS             31806..33020
                     /locus_tag="KRH39_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG8510319.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVNFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            33197..35110
                     /locus_tag="KRH39_000390"
     CDS             33197..35110
                     /locus_tag="KRH39_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8510320.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            35381..36355
                     /locus_tag="KRH39_000391"
     CDS             35381..36355
                     /locus_tag="KRH39_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG8510321.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            36376..36651
                     /locus_tag="KRH39_000392"
     CDS             36376..36651
                     /locus_tag="KRH39_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG8510322.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            36664..37827
                     /locus_tag="KRH39_000393"
     CDS             36664..37827
                     /locus_tag="KRH39_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG8510323.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGKIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            38165..40444
                     /locus_tag="KRH39_000394"
     CDS             38165..40444
                     /locus_tag="KRH39_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8510324.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(40539..41879)
                     /locus_tag="KRH39_000395"
     CDS             complement(40539..41879)
                     /locus_tag="KRH39_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8510325.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARIMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(41891..42673)
                     /locus_tag="KRH39_000396"
     CDS             complement(41891..42673)
                     /locus_tag="KRH39_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510326.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(42688..43710)
                     /locus_tag="KRH39_000397"
     CDS             complement(42688..43710)
                     /locus_tag="KRH39_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG8510327.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIVGVPYFIY
                     LMLVND"
     gene            complement(43716..44729)
                     /locus_tag="KRH39_000398"
     CDS             complement(43716..44729)
                     /locus_tag="KRH39_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG8510328.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(44857..45795)
                     /locus_tag="KRH39_000399"
     CDS             complement(44857..45795)
                     /locus_tag="KRH39_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8510329.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            46065..46529
                     /locus_tag="KRH39_000400"
     CDS             46065..46529
                     /locus_tag="KRH39_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510330.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(46641..46958)
                     /locus_tag="KRH39_000401"
     CDS             complement(46641..46958)
                     /locus_tag="KRH39_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG8510331.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(46971..47702)
                     /gene="chbG"
                     /locus_tag="KRH39_000402"
     CDS             complement(46971..47702)
                     /gene="chbG"
                     /locus_tag="KRH39_000402"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBG8510332.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKSVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(47728..49038)
                     /locus_tag="KRH39_000403"
     CDS             complement(47728..49038)
                     /locus_tag="KRH39_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG8510333.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(49115..50428)
                     /locus_tag="KRH39_000404"
     CDS             complement(49115..50428)
                     /locus_tag="KRH39_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8510334.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWSTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(50450..50761)
                     /locus_tag="KRH39_000405"
     CDS             complement(50450..50761)
                     /locus_tag="KRH39_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8510335.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
BASE COUNT        17151 a         8757 c         5848 g        19123 t
ORIGIN      
        1 tttatagatt ataaaatatc taactttcat ctacttcaaa gaatacaata cttccccttc
       61 tcttatagtc tgcaatactt taatatcttt tattttcata gggtcgattg taagtgggtt
      121 ttcatctaat attatcaaat tagctaattt cccttcttta attgaacctt ttttatcttc
      181 ttcaaaatat tgatatgctg catttatagt aacacccttt aaagcatcta atgggcttat
      241 cttttcattt tctcctattt gtataccttt ttttgtaatt ctattgacag cacaccatac
      301 tgtttccagc atattaggtg ctattactgg tgtatcctga tgaagagtgt aaattaatcc
      361 cttttctata gttgttttaa gtgggctaat cttaaacgct ctatcttctc caaggttttt
      421 gatatgaata tcaccccaat aatatgtgtg agctacaaag tatgaaggta tcatatttat
      481 tactttcata tcatcaatct ggtctgctct tactgtttga gcatgtatca taactggtct
      541 tatattattt tcactacttt gctcttttaa gtttaaaact ttctcaaacg catctatcaa
      601 ctggtctgct gctgcatctc cattacaatg agttaagagt tgcatctttt cttttagtga
      661 aatatctata aacttttcta cttcttcatc tttgtatata ggatatccac aataaccatc
      721 atctgaattt tcatatggtc tactcaacca tgctgtctta ccttgtggag aaccatctaa
      781 gaaaatctta taacctccta ttttaaatct atttatatat ttttttataa actccctatt
      841 attatctgct acagacttac tttttttcaa atttacatat cctacaacat ccatttttaa
      901 tttattttga tttgccattg cttttaaaat gttaaattga ttttcttcca ttaaaccttc
      961 ttgagctgtt gtaataccat agcttagata tatgttctgt gctttttcaa ttgaattaaa
     1021 aatctcattt gatgatggtt gttttatttt agatgctaca ttaaaaaatg cattttcttc
     1081 taaatatcca tttggttctt cactgccttc tactctacct atatgtccac cttctgggtc
     1141 acgtgtttca ttggtaacac caagctgttt taaccccaat gtatttgcaa ctcccatatg
     1201 accagaagca tgtgaaatta gtattggatt ttctgaagat gcactatcga gtacactctt
     1261 atctggatgc ttattttctt ctaaaaaatt attatcatat gaaaatccaa ttatccagtc
     1321 tccttttttt atattattcg actctttgaa atcctgtatt ttttttacta tgtctttaaa
     1381 actggttgca tcttctaaag gaactagctt tagagtagta gcaaaagcta tcaagtgact
     1441 atgtgaatct ataaaagatg gcatcaatgt cttaccttgt aaatctataa tttctgtatc
     1501 tttatttttc aaagcaaata cttcttcttt tgttcctatc ttttttatta ttttgtcttt
     1561 tataagaata gcatctccac atattgaatc ttccattgtg atgatatttc cattgtaata
     1621 aatttttgct ttcataaaat tctccttacg ttgtctactt taataaattt ttttcattat
     1681 tttctgctta attatattat cttctttata aactttttct ttagagttta atcatataaa
     1741 agttttattt ttaattattg tttgccaaga agtaaatact attcattgta aataagacaa
     1801 attaatagga tttatactaa ttgaaaaaat attataagtt gttaaattta aatatctaaa
     1861 ctcaattata tctgtacaat tgtaattttt taaatcatga tgtattattt taaatataca
     1921 gttacattat tattcacaat ataagtatct tagttctcaa ataacatctc atgtaatcta
     1981 ttcaatgcaa aaaatttaaa tattttttag gaggtttttt atgagcaagg aaaataaaaa
     2041 tgtaagaatt gctgttgtcc aggcgtcacc agtcataatg gatttagaaa aaactgttga
     2101 aaaagcatta ggattaatta aagaagctgg tagaaaaggg gcaaatatcg tagttttccc
     2161 tgaagcattc atacctgcat atccaagagg tctttcattt ggatttgttg ttggaagtag
     2221 aacaatggaa ggtagagaag attggaaaag atactatgat aattctgtac cagttcctag
     2281 tgctacaaca gatttattag gaaaagcagc acaagaagca ggagtatatc tatcaatggg
     2341 tataacagaa agagatggta atgatataaa ttgtaccctt tattgcacaa acttattttt
     2401 ctctccagaa ggtaagctaa ttggtaaaca cagaaaactt aaacctacag gaacagaaag
     2461 atgtatttgg ggagaaggag atggaagtac tcttacagta gttgatactc catatggaaa
     2521 aatgggttcg ttgatttgtt gggaaaatta tatgccatta gctagaacag ctctttatgc
     2581 taagggtgta aaactttaca tagcccctac tgcagattca agagaagagt ggcaagcaac
     2641 tatgaaacat attgctttag aaggaagatg ttttgtaatt ggatgcaatc aatatgttga
     2701 aaaaaatatg tatccaactg accttaacta ctataaagag ctagatgcag aaccagaaat
     2761 tatgtgtcca ggaggaagtt gtatagttga cccttttgga aaatatgtag caggtcctat
     2821 tttcaataaa gaagaaatgc taatagctga tttagaccta gaaaaaatag tacttagtag
     2881 actagatttt gattcagaag gtcactattc aagaccagat gtatttgaat taattgttca
     2941 tgaataatct ttttacacaa acatctacat aaatcttttt acataaaaaa atgagatact
     3001 cataagtaaa tttcaagtat ctcatttttg cttaaaacct ataatcctaa ttataaattt
     3061 attcacctat attttttcta aatcaactac tgttcttcca acatgttttc ctgccattat
     3121 tgaatctata ctatcattta actcttccaa tgatactaca tttaccatat catctaaatc
     3181 atctaatttc cattcatttg ataataaatc ccatattttt aatcttaagt tcattgggca
     3241 atccacagaa tctataccat ataaagtaat tccctttaat ataaatggat atactgaact
     3301 tgtaaaatca actcctcctg cattaccaca agttgttact gctccacaat aattagttgt
     3361 ctttattgct gttgctagag tatttcctcc tactgtatct attactccag cccatcttgg
     3421 tctaagcaga ggtcttcctg agttatcatc cacttctgac ctatgtataa catctttaac
     3481 acctaagttt agtaatactt ctttttgttc ttctaatttt cctgttgaaa ctacaactga
     3541 ataacctaat ttaatcaata ttttagttgc aattacagca actcctccag taccaccagt
     3601 gactaataca tctcccatat tcttgtctac agaatctata aatttataca cagataaagc
     3661 tgctgtaaac cctgctgtac cataatccat tgcttctttt agtgaaagat ttttaggaag
     3721 tttaactacc caactagaag gaactcttat gtattctgaa aaaccaccag aagtattcat
     3781 tcccaagtca taacctgtta ctaaaacttc ttctccaatt ttaaaatcat cacaactact
     3841 agattctact ataccagatg catcaattcc tggtgtatga ggatactttc tcgtaacacc
     3901 cttattacca tttgcagaaa gtgcatcctt gtaatttaaa gaagaatatt ttacttttat
     3961 taaaacttcg ccttctggca atgtatccac ttctctttca actatatttc ttctaaaatt
     4021 tccttcttct tctgtaatat atagtgattt aaatttttta ttcataaatt tcctcccaat
     4081 acatttagtt ttatgttaaa ctaattatag tttataaata gttttatgtc aaactaattt
     4141 aaatagatta aagattggag aataatttat ggaagataaa tatattgtac atttcataag
     4201 taaaacaaag gctagtatga ttaaattcat tgaaaataaa ctcagtaaaa atggtttagg
     4261 tgagttaata cctactcatg gaaatatttt aactgcactt tatgaaaata atggaatact
     4321 tacaatgaaa gaaattgcag ctaaaatagg aaaagataaa tccactgtta cagtgttggt
     4381 taataaactt ataaacctag gatatttaga aagacaaaaa tgtacaaatg acaaacgtat
     4441 cacgtatata aagcttacaa aaaaggcact gcttatagaa gatactttta atagcatctc
     4501 aacacaagtt aaagagacag cataccataa tattacagaa aatgaaaaac aagaatttct
     4561 tagactttta aaaaaaatta atgataattt taaaaatgct gactaaattg aacataatta
     4621 tatctcctat tggaataata aataaaaaga atcaaatatt gtaaaaattt attgtgatag
     4681 agaggtagaa tatgaattta aaagatagta aaactaaaga aaatttatta agagcttttg
     4741 caggtgagag tcaagctaga aatagataca atatttctgc atctgtagca aaaaaagaag
     4801 gattatatat aatagagtct ttatttaatt acactgctga ccaagaaaaa tcacatgcaa
     4861 aacaattctt ttctaaatta aaagaatttg ctggtgaaaa tataagtata tcaggagcat
     4921 accctgtaga tttatatgac aagacagcac aacaccttaa agctgcacaa cataatgagc
     4981 ttcaagagtg ggatgatgta tataaaaact ttggaaaaac agcacgtgaa gaaggatttg
     5041 aatctatagc taagttattt gaaaatatag ctgaaataga aaaagttcat ggtgatagat
     5101 ttggtagata tgctactgat ttagaagaag gtactttatt cagaagaaat gaagatatac
     5161 aatggatatg tacacattgt ggccatatac atataggtaa agaagctcca aaagcttgtc
     5221 ctgtatgtct tcatccacaa ggatatttct tagattttaa aaactcatta tttgaataaa
     5281 ttaaggcaat aattctattt aaacaattta ctatatttat atcaagtatt tttataaata
     5341 ctgtattatc ttaataacaa taaaatgaac cattgatatt actataagta gaaaatgtag
     5401 tttaaataca atttatggta gttcatcatg gttcatttta ttgtttacta atccctataa
     5461 cctacttaaa tctatcttta acttttacac actcacataa aatctcatct ttaaactaaa
     5521 tatttaaata aagtgtattt taagtagatt attataatac attatataag ataatacact
     5581 atgataactt tatacttata tcatttattt cttttgtcaa gtcaatctca ataatcttaa
     5641 ctacatcttt ttcaaacttt acttttttat aatccagaca tcctactacg aatggaactc
     5701 caaactttct aaacaaataa atcttcccac tttcattgta tattgatgta aaattttctt
     5761 ctattatatt cactatcaat tttaactctt caaaagttat tatagattta actaaaaact
     5821 tctctattat gctcaatata agcagttctt ttttacctat gctttgctct tttttatata
     5881 aattttgaaa tatttctttg gtataattac atataacttc tttactgaga ataaaatcaa
     5941 tgtcaaattc tttattaata tcctttttca taaacatatt tgcaatatca tcaagagaag
     6001 tctcttcttt catagacaat attaactcta ttctttctag tattttatct cttggaaaga
     6061 aagtctcttg acctgtaaaa gctgattttt ttataaacca ttcctctggt attatgtttt
     6121 ttcttttcca tctatataat tgaccataag atatattagt tttgctcaat aaatctttct
     6181 tagatattaa ttcttccata acttttctag cccaccttta tactgttttt tacagtagaa
     6241 ttatctgaaa ctgataaatt atcactgtat tcaatatttc caacatcaca attatctact
     6301 agagatatgc ttttacctcg tatatctttt attgatgcat cttctaattc aatagtatct
     6361 ccctctatca aattacattt aaactttcct gaaaaaatat taatgatttt aattaatgaa
     6421 aaagcatctt tgcttcttcc tatttttata ttagttgccc ctatctcttt acaataagaa
     6481 cctcctcctg aataaacata tatattttct ccatttaaaa gaccatcaca ttttattgct
     6541 ccatatacct ttatatcttc acattctata tttgatttat ttctcatttc gccatagatt
     6601 tttatatctt gtgcatacat attttcattt acacttaatt gtcctcttat tgtaacatta
     6661 ttacatttaa gtttgccatc cactttctgt tgagcagtta taactaagtc atctatttct
     6721 gcatctccct ctatattaag ttgaccattc actcttacac gctctgcttt aagattgcct
     6781 cttataatag tttttccgtt aacaacaaaa ttttttatat tgatgtcacc ttcaaatttt
     6841 gcatctccaa ggacttttac ttcgttactt ttaacatttc cttttgattt agaacttccc
     6901 acaattttta tattttcata ctcaccacta cttataaagc cttctccaag tatttttata
     6961 gacccttttt ccatattatt acctcatttt aaattttatt tttaaaccta cttattactt
     7021 atccttcttt aattttattt tttcacttat attctttatt tcaccaacta ttgaatcttc
     7081 atcaatctct atactttcac tgtactccaa actttctaca taacatccct tgcctatttt
     7141 aatatttttt cctcttacaa ctttgacttc actattttct aagcttattt catctgcttc
     7201 tataatattt gcaaacaatt tactttctgt atactttcta attgataata gacctaaact
     7261 tattaaattt gaagacactt tttcaaagtt gatatgcttt ttttctagca tacgaaattt
     7321 tttgcaataa tctccatgcc ccttttttat atttacacta cttgctccaa cttcattaaa
     7381 attagaagtt ccatttaaac taatattaac ttcttcacaa ttcaaaaatc cattacactc
     7441 caagattcca cttaaattga tattttcaca atctatattc tcatttgctt ttaaatatcc
     7501 ttctccatga ataatatccg attttatact tccttctaca ttaataattc cactagtatc
     7561 aatttcaaca gaattaatat ttcctgtaca tctaagaagt cccgatatct caacttttga
     7621 tgccagtatg tttccttcta ctttacttaa accagatgta aaaaattcat tacattctgt
     7681 tgtcccaaaa acttttgctg aaccagaaga atcaaattta ctacatttta cacttccttc
     7741 tattttgcct gttcctgata tattaacatt atcaaaaatt ccacctttac attttccaac
     7801 accatcaaat cttagtgaac ttaattcttc attcataatg atatcctttc tttaccgtat
     7861 tttatcttta aacctaatat aacaaaacat tgttacgttg tcaagtatga taataatact
     7921 taataaagca actatcattt attttctata aataggcata aaaataatag ggaaattctt
     7981 tatttaaact ataaatctaa tttcttagat tgtggtttaa ataaaatata tccctattac
     8041 tcaatcttta tatattcatt gtataaactt ctaaaatata agtctttaaa ttctactgtc
     8101 aatacttttt atacactgtt tgtgctatca tctcacacac ctaaaggagt gggctttcca
     8161 cactattctg taacctacaa gacactatct tcttctatct attgataaat ttttctatta
     8221 catcagctag tctctccata tcagttggat aaacttcacc tatgctacct attctaaatg
     8281 tatctatatc agtcaattta cctggataga taacaaatcc attatctttt aaataagtat
     8341 aaaattccat aaattcaaac tttgcatttt taggatataa gaaagttgtt attacaggtg
     8401 attgagcatt atcattcacc aaagtatcaa atcctaaaga ttttaatctt gaagctattg
     8461 taaactgatt ttctttatat ctagcatatc ttttttcaac agaaccttct tcttccaatt
     8521 ctaataaagc ttgataaaaa gctcttacaa catgtgtagg tgaggtaaat ctccatttac
     8581 cattattttt ctccatagtc tcccattgcg catacacatc aagagataat gatttagcta
     8641 tacctttaca tttagataat ttctctttat tagctattat aaaaccaaat ccaggtactc
     8701 cttgtataca tttgttagaa ctactaacta aaaaatctat attaaaatct tcaacatcaa
     8761 tctcaattcc cccaaaacta ctcatagcat caactatata aattttgtta tattcttttg
     8821 caagtttccc tacctcttgt ataggattta atctacctgt agtagtttca cagtgaacca
     8881 ttgatatatg tgttatatcc ttgttttctt ttaataaatt ttctactaca tttaaatcca
     8941 ctgcttctat atctttaaat gtgcaatcaa caaattctat gttaagataa ttacatatat
     9001 ctttcattct ctttccatat gctccatttg caattacaag aagtttacca tcttttgaaa
     9061 tagaacttcc tattatagct tctactgaaa aagtcccact tccttgcatt aatacacttg
     9121 tgtatttatc tgtattttgg gttgctagag atactaatct tcttctaaca tcttgtacta
     9181 agttattata ttcaacatcc caagtacacc aatcttttaa catagatgct cttactgatt
     9241 tagtagtaga tagtggtcct ggtgtaagta atatgtaatc attttctggt acaaaatctg
     9301 atttaatagt ttcattctct atttttatta gtatatcttc taactcagcc atagtttcta
     9361 ttacaaaatg tgctcctgcc tctttaaatt tctttgaaac tatactcatc ttagctttta
     9421 attcttcctt atccatgttc tctacttctt cttgtgtaag ccctaactca cttgaacctt
     9481 ttattacagc aacactccac attcctgcat ttacgccttc ttttacatca cttattgtat
     9541 ctcctacttt taccatagaa ctcataggag acacccctaa agcttcagca tttttatagc
     9601 acatccaagg atacggtctt ccttgagata cttctgatgg tgttacaaga aaatcgggtg
     9661 agtatccctt ctttgctgca tttggttcaa ctatgttcat catctctctt gtataacccg
     9721 ttgtagaacc tatttttaat ccattttttc tcaacttttc aatagtttca acaacatgag
     9781 gaattggagt cgtataatct tctaatgtct caaataacat tggctcaaat tcagcataaa
     9841 gttcatttac atcatcttca gttggcactt taccaaactt atctgaccaa agatttttta
     9901 tcctatccat ttcacacatt tctcttatat ggtctatttt taattttccc ataggttttc
     9961 ttgcttcttc cattgttaca tctatacctc ttcttttaaa tatctctata aatacattaa
    10021 gaggtgcaaa acatccataa tctactgttg ttccagccca atcgaatact actgctttta
    10081 ttttttctcc atatatcttt ttcatattta tagcctccat agattctttt tagaattttt
    10141 gtgtttttaa ttgtttttac ttttaaagta taactttcat atgttaatta tacatcatat
    10201 ttaggttaaa tatatattaa atttctgttt ttagttgcat ttagttgttt ttattttgta
    10261 tcacattccc ataaaatcct aagttacaat ctaaaattta ctttctaatc ttaatctatg
    10321 aactgactat attttatgaa tttagttgta ttgagcatag ttgtattata taaaactaag
    10381 ggtttatttt gcttctatcc atgttatatt tatctcattt aacttatcct tccattcatt
    10441 cggaggttct acatcacaaa taacaaaatc taattcctct aaatctgcta tcttatacat
    10501 actagcaatt cctattttag aactatcaca taatacaaaa ctttcctttg aattttttat
    10561 atattctttt gacaacaatg ccttattttg gtctatacta ctaattccaa aactatcaag
    10621 tataccttca ctagatataa aagctttatc tacgtataag tttttcatag tatctattga
    10681 tattggacat gctgtccttt ggtgttttaa atttactttt ccaccaataa atataacttc
    10741 tccatcaaac aaaccttgat tctcataaga aatcaatttt gatagaactg gataagagtt
    10801 tgtaataatt tttaaattgt ttacagatat tatataatct acaatctgca aattggtagt
    10861 accttcatct atgataattg actcattgtc atttacaaac ttagctgcat atttagcaat
    10921 actttgcttt ttatctaggt tagtattgtt tctctttata tgttctggtt caatcactcc
    10981 ataatttatt tttatagcac caccatatac cttttttaat ttattttcat tttctaattc
    11041 ttccaaatat cttcttattg tttctgatga tacttctaat ctttttacta attctttagt
    11101 gtttaccttc ccttttgaat ctaataattt aagtatttct ttttttctat ctatcgaatt
    11161 taaagacatt cattttcctc ctaaatataa tatatttttc tcctataata ttacaatgat
    11221 acctaagatt tatgaaatca tatttatgta ttaaattttt tacttaatta aagttatttt
    11281 caaaagactt tgtattatca ttaaaaaagt ccatcaaata ttagtatcag atggactttg
    11341 taaaatttta ttttataaat taatatttaa agtctaacta ctaatttatt ttcttgtctc
    11401 caagaatttt actaaattat ttacagtaga catatctttt ctctctaaat cagttggttg
    11461 aagctctatc cctaatctat tttcaatttc aagtaaaact tcaattatag ctaatgagtc
    11521 taaaagttcc gtctcaaata aattaaggtc taaatcttct cttatctcat cagtccctaa
    11581 tacatcttca aatatttcta taactgtttc ttgcataaat tttcctcctt aaaattaagc
    11641 taaataacct gagaatatca gcaatccaaa acacacaata tggaatgtaa tgaaaatctg
    11701 cactctctcg aaccatttat cttttttatg ttttttataa aatttagatt ttcgctgata
    11761 tatatcagtt aacacaagtg ccaatccttg gtatacacca taagcaacat aataccaagt
    11821 tagaccatgc caaaatccca ttgttatcat ggttatcatc tgagccacat gagcagcagt
    11881 agtccttttc ttaaatcttt tctttctcat tgatgacata acaaatctag aaaaaatata
    11941 atctccaaac catcttgaaa gacttatatg ccatcttgtc cagaactctt tcatatcttt
    12001 gctgataaat ggcttgtcaa agtttatagg tacttgtatt ccaaatatgt accctgtacc
    12061 tatagcaaat aaactatatc ctgcaaaatc aaagaacagg tataaactat atgcgtacat
    12121 ataacttaaa atatttataa aactcatatc tttaggtatt cttgataccc aatatgtatt
    12181 tatcaaaaat gctataacaa acttataacc tactcccatg acaatatttt ttattcctgg
    12241 cagtaagtat tcttctatat actcttttct agagatttgt ttttccaagt cctgttcaaa
    12301 tcttcttgac ctatcaatag gtccagaact taaagttgga aaaaataaca taaaataaag
    12361 catctttgaa attttaactt cttttatagc tccatcataa atttcaataa ccatttgtat
    12421 agttctgaaa tttaagtatg atattccaat aaatccaatt attccaaaag aagtaactgg
    12481 agatatttta tttattatta ttgggagcat tgatgcaaac aagaatcccc aatatatgta
    12541 tttattttta gtttttcttc ttacatattc atatccttta actattatta cttcaagaat
    12601 aataaatatt accaagtact ttagttggac atcaattcct actattaaga atatcataaa
    12661 caaacttgcc aacataccat aatactttat attcttgccc attagaccta aaattactgc
    12721 tggtattgat gttaataata atatataaag ataaaaataa tctccatatt gtgaaaaaat
    12781 cattaaattt cctccataag tttctttctg tctattttac cattgatatt tgttggaaac
    12841 tcagagatta ttgaaatatt tcttggaatc atatatgaag gtatatactt tccaagttcc
    12901 ttttttatta tcatcccatt tttaatatta cttaaatcat tcttttcatt taactcaaca
    12961 atacctttta agtaagcaat tttttcgtcc ttatatactg gtaaaactac tgcatttttt
    13021 acattatgta cttttctcaa attattttct atgtcttcta tttcaattct aaatccattt
    13081 aatttaatct gaaaatcttt tcttccacaa taatatatat ttccatctaa aagatatccc
    13141 atatctccag ttttataagc tctccacttt acaccatcta tttcatcata gaaaaatact
    13201 tcatcagtct tttctttatt attgaagtaa cctttagaaa ctgatggtcc aattattata
    13261 atttcaccct tttcattttc tttaagctca tttccatctt catcaagtat ttttatttta
    13321 caatttgaca tagggtatcc aactggaaga cttttttcat catctatagc cttttgagtc
    13381 atatcattta cacttacacc aacagtagcc tcggttggtc catatccatt gataattctt
    13441 gtgtttggaa atctactcat aagttcttta gtaagatttt ttgaaagtgc ttctcctatg
    13501 aatatcattg actcaaggtt aggaagcatt ttactattaa actctttttc agttatacat
    13561 actcctgcaa aagaaggagt tgacacccat acagctatat ctgagatact aaattgtcta
    13621 aatagttctt tatagtctgc taatacatct tttgatattg aaaataatgt agctccatgg
    13681 ataagaccag ggtatattgt tgtaactgaa aggtcaaaag aatatgctgg ttggttcatt
    13741 attacttttt cacttccatc tatgtttaga tatgggctta tccaatctga aaagctatct
    13801 aaattgtttg aacttatttg aacccctttt ggctttcctg tacttcctga agtaaatagt
    13861 atatatgcat tttcatcatc ttttacccag ttttctttat ctaatgattt tccctgatat
    13921 tcatttatta tataatttaa tttatccata tcaattacat tgatatctcc aaaatttctt
    13981 tcatcactaa agttaaataa tacctttggt tttatctctt tagtgacttc aaaaactcta
    14041 tcaattggaa aactaatatc aagaggcaca tacgctctac ctgattttaa agctcctatc
    14101 atacatgcca ttatcatgtt ttctttattt ccataaatta ctataggagt atcttcttct
    14161 ttgtatacat cctttagaaa tactgatata gcatctgaat attcatttaa atctttataa
    14221 gaaagtttat ctccattaca cattagtgct gttctatctg tatttgaata tttttttatt
    14281 ccttcaataa ttttcatata agaatgctcc tttaaaatcc gttataaata aatggcaatt
    14341 ctttaaatgg agtaaatgtt agaactccta tcgccaatat tataaccata gttaaacaaa
    14401 tactagttaa caaatcttta ttgttcttta aatattttaa ataatctctc acagacatct
    14461 acccatcctt tcgtaccaag atgcattaca tcccttaaat aatacttatc tgacccttta
    14521 tctttaaggt ttagaacttc aaagcctttg ctttctgcta ttttttgtgc tttatcgtag
    14581 tactgatttc tttctttttc agaaatacca gtcaaattat agaatttatc catacttggg
    14641 attaatacaa caactggctt aatacctaag tcagtacata catttagcat cagtttataa
    14701 ttttcaaact ccttagatgt taataaattt acatctttgt ctcttccttt gtattgctct
    14761 ataccatctt taaaatgctc tttataatag tacttatcaa tatttagtgg atttttacca
    14821 actctttttt tagcatcttc aatggctttc tttctctcat atgaccaatt tattggtctt
    14881 tttctttttg ttgctctctt tttatctagc ttaattaatc gtttataaag aatacccttt
    14941 tctttaagag ctatacaata ctttcttcct tgaaaataag gctctaaaag caccttttca
    15001 gctttttcag gaaatgtctt aggctcatat agcttagcat ataatgcttc tgctttatac
    15061 tcgtcacttc cccataataa cttacttgat ttttttgcat attcaatctt attttgttta
    15121 gatatttttg gattatctaa aaatcgataa aattgtattg gtgaaaatct agactgataa
    15181 tggtgacttg tgactccgtc tttttccata aaccattgca tagaaattaa caagacaact
    15241 ttcttattat caatattagg attcatactt cctaatattg cagcatcttg taatgtctga
    15301 gtataggctc tcccaattgc aaacacctta tttttactcc tatttgtatt aaaatagtac
    15361 tttggatgtt gttttgttga attactaagt tctgatgacc ccaaaatcat aatatccttt
    15421 tctcttaaaa aatgattatt tgctgtaacc cctttatctt ttatatcact taaagtatca
    15481 tccataattg gaagtagatt tttctccctc aaaagttcat cagttttaga atcaagaaat
    15541 ttgtctaacc caaataaaaa tactacgcct attataaaag gtgtaataaa gtatatcaat
    15601 tttctcatat tttctctcct ctaaaaatat tcaaatttat gttattatta atcatattag
    15661 ttttgatatg tatattcaac tacaaaattg taataaaatc gtcaaaactt tttttgatac
    15721 tgttacaaaa ttgtaaaaaa aaagaaaaaa acgccaaaaa taaatttctt tttagcactt
    15781 tttcatactt ataatttgac attttaacac ttggttaagt atacacctta ccttaactta
    15841 aaattaaatt tatttaagaa tatatattat aaattcttat attttctaat ataaactata
    15901 tactatcagc tggataaaca atactacttt gtcttcttat ctcatccata atttccatta
    15961 ctattattga attttcatga gaatttatat ttgattctat tttattgtta tttataagac
    16021 ttataaactc tacaagttca taatacattc cttctatttc tctaggcttt tcctctgctt
    16081 tttcttttgg aatagatata tcttcttctc ttccatctct gtatttaata atagcttttt
    16141 caaatagatt aagcttttca attattatac ttccctcttc tccctgtatt tcagatggta
    16201 tataagaatc agctacttta gaatactgaa ttatcgcatc catatcatca tattgaaata
    16261 cagctgaacc ttctccatcc actccagttt gaagtatatg agaaactgcc tttacagttt
    16321 ttggagcacc aaataaattc accataggat gtatacaata cactcctata tccattaaag
    16381 ctccatttga caactctttt ttaaaggcat tttctataat tccattttta tatttatcat
    16441 atcttgaaga atactgacaa tagcttccaa aaaatcttct tattttccct attttataca
    16501 agttttcttt aactgcttta aagtttggaa cacatgtaat tctcatagct tccataagca
    16561 atacattatt atctttggct gtttttatca ttgatttgac ttcttttaaa ttagatgcta
    16621 acgacttttc acaaagcaca tgttttttgt gttttaaaca agtaatggtt tgctttgaat
    16681 gtaatgcatt tggagaagct atgtatacag catctatatc cttcgattta gccatttgtt
    16741 ctaaatcatc aaaaaaaata cttgctccat atagagttcc aaattctcta gctttttcta
    16801 aatttctaga atatacagca accaattcaa agtctttaac cctagatgca gctcttagaa
    16861 atatatttgc tatattgctt gtcccaacaa taccaaatct tattttaccc atcataaatc
    16921 actccccatt ttctacactt tattttaatt ttaataaaaa actttacaac ttatagatat
    16981 attataaaat aattttatca aataaaaaag aaagctaatt ttaaatgatt tcaccatttt
    17041 aacatagctt tcttttaact acaatctaaa ttcaacttat atttttaaag ttatctttct
    17101 actatatctt tatttcccct caaaaatttt atagtaagaa tttcatccac aaacaatgtt
    17161 actaaaagta tagttacaac aattggccaa taaggtatta taagactata taaactatca
    17221 actatataca cttgaaaata tctaactcca ataaatgtca atatagtcca atatacagaa
    17281 aactttagac atactattcc tctactattg tacttataat ctgaataatc ccaatagacc
    17341 ttattaaaat atactttcat aagtattcct gtaatatatt ctactactgt cggcactact
    17401 agacataata aaagcaatgc aaatatatta ttggggtcaa taaatcttga gacctctatt
    17461 ataatcgaca ttgcaattgc atacattggt ttaaatggac catttaaaaa tccatcttct
    17521 tgaaaatgcc cttgtttata ataactatat aagttttcaa ttatccaacc tataaaacca
    17581 tacaagaaaa aattaaataa aaaatataat agagaaccta cattatccat gaatattcct
    17641 cctagctgtt tatacttatt ttttctcata ttcatggatt tattctaaat caaagaaatt
    17701 atatttaatt tatttaatta atactttata ttatagattg tgacattctt tagagttcaa
    17761 actttttagg tattctctaa cttccagtaa attttcaagt atcttatgtg attgtgattt
    17821 aatttgctca tctggctctt ttaaatctgg tacatttata catcttatac caccattgta
    17881 tgcagcttca actcccatag gcgaatcctc tatgacaata caatttttgg gattaacatt
    17941 tatcttttta gctgctttta agaatatctc agggtttggt ttagagttta caacatcatc
    18001 tccacaaact atagcatcaa agtaatcttt taaatttgcc ttagctaatc tctttactgc
    18061 tctttctctc tttgttgatg ttgcaacagc cattttgtat ccattttctt ttaaaaaaga
    18121 gattagttca ttgactccta atttgatagg agctcccttc ctctccataa attcaatcat
    18181 gtttttagtt ttttcgtcat ataaatctat tattggtaca gaactatcgt atatatctgt
    18241 aagaccttct attattcctt tacgatttct tcccataact gatgtataaa tttccttagt
    18301 catagtatat ccatactttt caaacgtctc catccaaaat tcaagataaa ttctctctga
    18361 gtcaaacaaa actccatcca tgtcaaaaat aattccttct actttttgca tacaattcct
    18421 ccaatatttt ttgatataat tatattacac tttttatgat taattttaca accctcaatt
    18481 tatccaattc taatattgta aaattgtaat tttcaaaagt tacactttgg tttactttaa
    18541 tatttgaacc aagcatatag gatatccacc cacctatact gtcaaatcca tcatgctcta
    18601 tatcaagtcc aaaatactta ttaatatagt tgatagattc agttccatca actaagtaag
    18661 aacctgctcc tattttctta atatgagtct cttctttgtc aaattcatct tgaatttctc
    18721 caactatctc ttctagtata tcttctattg ttaccacacc agatgtacca ccatattcat
    18781 ccacaactat agcaagttga agtttttcct ttcttattct ctcaagagct ttatctatag
    18841 ttaaattttc agatatatat ataatatctc tcaatatttc ttctagttca attttatttt
    18901 catttatctt ttgattgtat aaatctctta tatgtacaaa acctaatata tcatctttat
    18961 tttttctgca tactggatat cttgtaacac cttcctcttt taaaatagct aatatctttt
    19021 cttcagaatc actttcatat atacatacca tatcagttct aggaaccatt atctctctaa
    19081 ttttcttttc ttcaaattca aaaatattat caactaatct ttgctctgat tcatctataa
    19141 gtccactttt ataactttct tctactaata gtcttatttc gtcacctgta tggggttcat
    19201 caacctcatc agcttgtgaa tatccaaatg gtttaagaaa taaatcagta cttaagttga
    19261 aaaaatatat tattggatat gttaacttat aaaatcctac taataaaaaa gatgtactca
    19321 acattatttt ttctgtatta taaagtgcta aagctttagg aaccaactct cctattacaa
    19381 cctccatcat tgttataatt aaaaaagaaa tggaaattga tatagtatat ataatacttt
    19441 cactaagatt aaatacactt actattggta aaattaactc tttaactgta gattctccca
    19501 tccaacctaa tgcaagtgaa caaagtgtta taccaagttg acatgcagaa agataggaat
    19561 ttaagttgtc ttttattatt ttacatcttt ttgcattatt attaccttca gttatcatgg
    19621 tatcaatcct agaactacga agttttacca tagcaaattc acaagctaca aaaaaagcat
    19681 ttatcaaaac aagcattact ataagtgtta cttttaataa ctcccgcata aagtattggt
    19741 tcttataatg taagaaatct ataagaactc aaagttcccc ccttatatta ataaatttaa
    19801 tttataatat atattctatc atatttatta atatatccta ctttattaca aataattttt
    19861 ataaactaaa taaccccttt taatatttat gactggctat ttgataaata ttaaaggggt
    19921 tatttagtta tcatttttta tttctaaaaa tcttttctca aatgatttag ttatctattt
    19981 atccaatttt ttacataatt taatgattaa aaatattaat tttttcacta taaagtacaa
    20041 atattatctg attattaaaa taaatattaa tactataaat aaaaacgaag catgattttt
    20101 atttaaataa atctcaaact tcgtctttac ttacttagat atttatttac tttctaaatc
    20161 ctattatttt taaaacctta tctttttaat acttcaccac atttattctc agtaatctct
    20221 ccattttcca atgcaatttt tccatttaca attacatatt caacaccatt ggctagtaat
    20281 tgtggcttcg aaaaatcaat atcttctgac ctagaataac tatagttatc catgtcaata
    20341 actgctatat cagctatcaa cccttcttta agaatacctc tattttttat tttaaattgc
    20401 ctagctggta atgaagtaac tcttcttaca atctcttcca atctaacacc agactttctg
    20461 tacatattga agaaaattgg aaaagctcct cttctttgta gttcaaacca tgacatatcc
    20521 ccacccacac taaacaaaca atctgaacca accataacaa atggattatc aattatttta
    20581 tcatcatgta gtttatctgg gaaatcagct ctatacattc ctcctaaaca aaagataagt
    20641 tcttcgtctc catctaaaag taaatcgagt aataacttgt cctcatctat tcctttttgt
    20701 gtagctattt cacgtattga tttcttttcc atattaatat ctttagtgtt tagaagtatt
    20761 atactccccc tatctccaag tataaatgct tcttttaata taagtgttct tccctcttca
    20821 tttctaagag catccattac cccatctata cctaattcaa atagactaga tgatattgcc
    20881 ataataaatt gaattactct ttttttcttc atacaatgcc cagaacttct tggtactgtg
    20941 tcaaccatta tttcaacacc attataccta gcctttaata tctcatcaaa tgcttctatt
    21001 tcaactggac ttaagtgaga tacttgaacc ctagcaccac ttttttgccc aacctctatg
    21061 gcttctttta ctgcattata tcttcctatg taatctctaa tatgagatgt atatatacca
    21121 tcatactctt taatctgcct tgcaatatca acaagttcat caatatcagc atacttactt
    21181 ggtatatatg ccaatccagt agatattcca aaagcaccac ttttcattcc atcatttatt
    21241 atatctgtaa tttcattttt ttccttttct gtaggaggtc tatccttaga acctcccata
    21301 acactccatc ttatagtccc atgccccaac aagaatccca tattgacaga aataccactt
    21361 ttagatataa ggtcacaata ttctgtaaaa ttattccaac atttattttt atctataaga
    21421 tacttttttt cttcttcaag taatagacca tttttataca tatattcata cacattttca
    21481 gaagaatatg gtgttataga atgtccacaa ttaccattta tagttgttgt aactccttgt
    21541 tttagaaact tttcaaattt accatctagt attgctacta tttcttcatg aacatgaggg
    21601 tcaataaatc caggtgttat aacttttcca caacaatcaa tctctttatt tatattgcta
    21661 tttatcttag aatctatttt tgctattaag ttgtctttaa ttgctatatc tccataaaaa
    21721 ccaggattac ctgtaccatc tattataaat ccatttttta agattaaatc atatatcaaa
    21781 atattccctc cttaaaaacc aatttttaaa taatcttaat gtaattttta tatttgaaaa
    21841 tcaaattatt aaatttcata tttgaaaatt cactattatt tcattgtaat tagtcactaa
    21901 gcttatcttt atacatagct cttgcaatta atatagtacc aaatacacat ataagaacta
    21961 ctagagactg tggtattata ccttgctttg ataatataat agtcaataca gatattacaa
    22021 ttgctactag acctaatttt ttatcttgta aaaatacttg tccaaaaact gacccaaata
    22081 gagctggtaa aattaagttt aatttttcta ctacttcagc tggaatttta gataatacgc
    22141 ttcctcctaa aattacccct atagttagta tacttatatt cactaaaata gatacagcta
    22201 ttcctattgt agatattatc gagccttctt ctgtacctga ttcaacttct gctgcctttt
    22261 gagctgctat tgaacatggt agcctcatat tagatatatt accagaaaga aaactcatat
    22321 aagttcctgg tatacctaag attggtgaat aagaaactgg ttctattatg taatttggag
    22381 cactcatact gacaattgcc attgtacttg ctaataatgc tgatattgga ggcataatac
    22441 caaatccaaa gctcaaaatt attccaggaa ctaatgctgc tataataccc aaagatagtg
    22501 ttagtcttcc ataccttatc ataaaaggta aatattcttc attgtatata gattttactt
    22561 tactcataaa actcccccct tatattactg aagctattat cattccgcta aacattgata
    22621 ttgtaagccc ccactctctt aaccatttta tatttttctt cttttcaagc atacacaata
    22681 ttaccattat agccattcca cttatacatg caattgttcc attatcaaaa cgcaacaccc
    22741 tatcagcatt tagatatgca aatgaaccca acattgctcc tagagatatt attggtataa
    22801 atgatttctt gccactagat ataacattgg ttatcttatc tagtttatgt ccaaatagta
    22861 atgtgaatat tatccatcct aaagaaccta atatcatagt ccaaactgca tttgtaaatg
    22921 caagttttgt catttcagga gaacctaaag ttatacctaa tgcatctgtt ccaaatccgg
    22981 ctgacattga ttcaaaaatt acagaaccta taaatgataa tctcatccat gctataggcc
    23041 cacccatagt tactataagc gataccattc cagcaaatat agtcattgaa ggcccaatag
    23101 ctgaaatagc actactctta acagctgaat ttatttgttt atctgttatt cccattgttt
    23161 taccaacttt gcaagatttt atagcaaata taagggattg taccactact attgatactg
    23221 ctactgtact cgctatccac aaaagtggat gattagctaa ttttaaataa tccatttata
    23281 aaacctccct ataaatcctc aaattttatt aatatttcta atatatctat ttccaaacct
    23341 gttttagtac tctaagtata tttccaccta gtaccttctc tatttcctca tcactatatc
    23401 catgctttac taaatatcta acaatgtttt ttgaagtttc tgttgggttc tcaagacctt
    23461 ttacatattc tactttggga tgttcttttg ttcctgttat tttttttata tcaaatttag
    23521 cagctagtac tgagtgtaat tttacatggt cgccatatac tgtatctggt ccaaaagcta
    23581 catggtctat tcctactaaa tttttaatat actcaaaatg ttccataaat gactctattg
    23641 aatgttcttt attcttctct gttattgtag tatgaggtgc tgcttctaca cctataaccc
    23701 ctcctttttt agcacatgct attaatactt catctggagt tagtctcttt gtgttccata
    23761 aacttcttgc cccaacatgg cttaatatta ttgggtcctt gctatattct attacatcaa
    23821 gtgtagtttg aactccaacg tgagagcaat ctattgccat tccaatttta ttcattcttt
    23881 caacagcttt tttcccaaaa tttgtaagac ctccatcttt ctcttctttt agtcctgaac
    23941 ctaatgcatt ggattcgctg tatgttatac ccattaacct tataccaaat ccatacagta
    24001 tatctattct gtcaagttca ttttctattg gtgctgcacc ttctaatgtt ggtatcaatg
    24061 ctactttacc ttcactttta gctctgtata tatcttctac tttttcacac tttatgacaa
    24121 aatcttgatg agctaaatca catagtctca tgcctaaatc tattaatata tcatcccatt
    24181 tccaccctga atttgatgtt atcgtacata caccatccat taaattatca aatatagcat
    24241 cataatatcc tcttgataat gcttcataag cacatctttg tttaccttcc ctattatatt
    24301 caaatatatc tctttctagg tgttctggaa ataatactgg atgttcatgt aatgatataa
    24361 atattttttc atccactata cttttaacct tagcttcttg aatatcatct aattcaataa
    24421 ggtactcttt aactctttta tctcctttac acatctcaat agatgaaata tcttttcctt
    24481 tttctaaata actataggct tcatatcctt tgtaagctga cttcataaca acatcctcca
    24541 agttctacaa ttttttatat ttttcctatg tagcaattga tgtgccaaaa tataaatttt
    24601 aattgtataa taaatcttgc aaaaaataaa aacagtagta aggttgatat ttactactgt
    24661 tttattaaaa ataatcttct tagattattt attcaatatt tttgaattat cttaatctta
    24721 aagataattt tatttatttc tatccattat agggattttt ttagggtgtt ttgccttttt
    24781 agtcccctat atcactccct taatttttac atatgatata aagttctttc cttcatcagt
    24841 tataaaacta ccttgttttt ttactccaga taaaagtaaa ccatggctct ttaacttatc
    24901 tattcttttt ctaatttggt ctacacttaa aactatatct ttttctttta aaatctcttg
    24961 cagtttattt cttcctaata atatattttt attattccaa gtttctatac tggtcaatat
    25021 acatatactt tcctcaaaga aattagattg tttgaaatct aagataattg aatcgaaatt
    25081 ttcattaact aaagtattgt ttttttcaaa tctaaattgt tctggcaaat gctcataatc
    25141 tactctatct tcctcaacta tagtatcaat ataataaatt aaatttttaa gctctcttac
    25201 atttcctggc cattcataca atgttaaagc ttgcattgat tcctctgtaa aacatttgtt
    25261 gctatttatt tcatctaaaa aatacttact tataagaggt atatcttcct tacgttctct
    25321 aagtctaggt atctctatat gtaatacatt aatcctataa tacaaatctt ctctaaaaga
    25381 accttcttta atttttttct ttaaatcttt atttgttgca gctataattc taacatctat
    25441 tggtataatt ttacttccgc ccattctaat aacttctttt tcctgtaata ctcttagcaa
    25501 tctctgctgt acatctaaag atatatcacc tatctcatct aaaaaaattg ttcctgtatg
    25561 tgctctttca aatataccca tttttccacc ttttattgct cctgtaaaac ttccttcatc
    25621 atagccaaaa agttcacttt caataagtgt atctgacaaa gaagatagat taactgcaac
    25681 aaatggcttg ttttttctta gagattcatt atgtattgcc tgggcaaata tttcttttcc
    25741 tgttccattt tctccaagta tcaacacaga aaagtctgta gttgctattt ttcttgcaat
    25801 attaattttt tcttttatta ttttactctc acctacaaca ctctcaaatg tatactttga
    25861 tacaaaacct tttgactgaa gtttattttg aatcttgtcc tcaagcactt gaattgcact
    25921 tatattttta atgcttataa tactctttat tcgttcatta ttttcataaa catttacttt
    25981 attaattatc agttttttgt tatttaaatt tactacttca tcattaactt catcttcttg
    26041 aaaaaatatt tttttgacaa ccttatcact aaataagtcc ataaagttat tagatataat
    26101 ttcattttgg tctattccaa ctagattaga aaatactttg ttgcaatata taaatttccc
    26161 taatttatca acagaagcaa taccttcatc tataatctcc aaaaaactct tcatggtttt
    26221 attcatagtt gtataatacc tatatccact tactgtatct tcattgtatt tttccttaat
    26281 aatatgtaat ttatctatag atatatttag ttttgtaaag atttctataa ctgtatttat
    26341 atcaattacc ttatcaccta tgtcaattat ttgctttatg ttttgtggta tgctgtttct
    26401 gcttcccgta ataattccaa tctcacattt agacttatca caacctggat aatatggtat
    26461 tagattaata tgaattaatc ctagttttct aattatcttg gcagtttcat ctgctgactc
    26521 tttataagca tctataacca taacatcaga atcattttct atacttatta tctgatttat
    26581 attttcaata tttatagtac gatgtactat tacaataggt atatttttat caatattatt
    26641 atgtatatat tcctttatat cattgtctga acatactaca agctcatagc ttgacagttc
    26701 aatatttatc ctatttacat ataccaagtt atctatatta caaaattctc caaatatatt
    26761 ttccaattgt tttgatatat ctatatttat tttgtcatca gttgatacaa ataaaatatt
    26821 atgttttttc atagtcccac ctcctgttta acgagtatag aaagtaataa tttggtctat
    26881 tgtagcactt tttaaaaaag ttttaaatct taaaaatcaa attttgtacc atttactatt
    26941 attttccata ataaaataat gtaagctatc ttttttcaca tataatttaa tttctaaaac
    27001 ggttagtttg acattattgg aatataaaca aaaatttaat ataaattcgt taatatatta
    27061 atacattttt aagaatgaca aggaataaat tataacaaat tttaggaata tataataata
    27121 gggtctcaga acttagttac aagttctaaa accctatttg tttagtgtaa aaaacatcat
    27181 aaaatttagc ttaaatcttt tcacataata ttactctttt tctaagtgat aataaaattt
    27241 atctattcct ctaaaaactg taaaaattcc tgattaaata gtttaagctc atcatagaat
    27301 atagcatgac cactatactc aaaagtatat aaaattgaat cttctatttt ttcattcata
    27361 aaaactgcaa attcatacgg acatatttca tctaatttcc catgaaatat accagtaggc
    27421 actcttacac attttaaatc attaaataat tcttcatctc ttaaagatac agcagttcca
    27481 atagtgccaa tacctgatgc actaaaacca atatcattaa accatcttct aaagctttct
    27541 ggtggatttg atgcaaatac tttttcgcca aaatcggtta ccatttctgg tctatcagta
    27601 catgcttgtg ctatcaattt atttacatct tcacgtgtca taccatatgg aaattctgga
    27661 ggtctttgta caaatgatgg tgcagcagct gcagctaata ccaattttga cactccatat
    27721 ccattaaata aactcatata cctaagtacg attgctcctc ccattgaaaa accaacaagg
    27781 gtaaagtctt ttaatccaat agcatgtacc accttataaa tatcgtctgc taattggcta
    27841 taagtatatc ctcctgatgt tgcatcagat ttaccaaaac ctcttaaatc aattgaaact
    27901 gttctatatc cgagttttgg caatatattt gtttgatatt caaatatctt atgtcctaag
    27961 ggccaaccat gtataaaaag tacagttttt ttagcactcg gattcaaatc atatactgct
    28021 atgtttatat cattcacata tacataatac atatcaacag caactccctt caagaaaatg
    28081 tatgatggtt attttatata tatgaataaa atgatgaata actaaatttt caaatttatt
    28141 tacaatactt ctctattgcc ttttcaaata aacttacatg aagtttttca tctaggatta
    28201 ttctatttaa tatagctttt atataagggt cttcaataag acttatatct ttataataat
    28261 tattaatagc cacatgctcc aaatgcaaat ctagttttaa tctgttacaa atagaatttc
    28321 cataacatac aaaaccacca ttccaatatg caccacaaga acttgtagaa cctctatata
    28381 caggatttcc acccaataat cttatagtct ttgctaatat ctccatatgt aacatttctg
    28441 ttatagatat atttatccac atttcactta actctctatg cgtttcatct aaatcaaaat
    28501 tatggtatag atattgattt acactcgtaa attcacttga tactccggca tagtcatcca
    28561 tcaataattc tgcataatac ttatttggac ctaaaacttt tatctctgga tatggctcat
    28621 cacttgaata tccatttcgc ttgtgcatgt cataatgtgc atcactcata atatctcctc
    28681 ctttaaattt aatacatgat attaattgta tgcatcacga ctttttcata taacttagat
    28741 aataaaatct acttaaataa taaaaagcca tattaattaa aattaacatg accttttatc
    28801 cacaaaatta ctttttaatt attcatcttt tccagctact gatagctctt taataacaac
    28861 tgaaggtgaa cctatactac tcattggaaa ttctaaatct tcccctatag cttcaatatc
    28921 tttcaacagg tcaaaatagt ttcctgctac tgtaatttgc tctactggaa atactttttt
    28981 tccttctttg atataaaagc ctttggcagc taaagaaaag tctcctgtta ctgaatttgc
    29041 acctgaatga agtcctgcaa agctagtaac catcaatcct tcatctatat cctttataat
    29101 ttcatctaaa gatttatctc ctttctctat ataaaaatta gttggatata tgcttatagg
    29161 tgatgaatat gaagatttta ttccatttcc tgtagtcttt acacctgcct tattagctgt
    29221 ttttaagtta tgaagtaaag ttataagctt tccatttaaa acaacttctt ttttaaatgt
    29281 agctactcct tcatcatcaa aaggagtaga tgccatacca ttttctaaaa gtgggtcatc
    29341 aactattgta actataggag atgctatcat gtctccttct ctatttttca ataaagataa
    29401 tcctttttga gctgaatctg cattaaatat cccagaaaat gtgctaagca aagataccat
    29461 agcttcatta agcaatatag ttttatattt acctgatgga atactcttac cacctacttt
    29521 agataaagca ttatcaacac caagttttgc tatctcacaa ggttttattt cttcaataga
    29581 atttgctata ttatatccta ttccatcata tttttgtcca ttgtcttcta tgataggaac
    29641 tacaaaccct ataagcatat tagttttatt gcttaaatta agacctttag tattagatat
    29701 accattactt gatacagaat aagatatagt acatttagat aaatttacca ccttatctga
    29761 ataagcttta gtctctcttt ccatttctaa agctaaatca atcaacttat ctgcctctaa
    29821 attttctagc tctttagaat aagtctttac atcattatag tgcttatctc cttcatatat
    29881 aaattgtaca tcttcatttt caatagtgtt tactccatct tttacatttt ttatcaacat
    29941 atcaacagct ttatcatcta aaatttctgt ataagaataa cctatctttc catttacctt
    30001 acctctaaaa gataaaccaa aagatttatc aagattgtat ttctcaactt ctccttcata
    30061 tatatttata cttaaatttt ctcctgtata ataatatatt tcacactctt caaaaccttc
    30121 acttagagcc tttttaaata atatatcttt aaaattttta agttccacaa ttatccctcc
    30181 tatcttccac ctacagtaat ttcttttacc cttatcatag gttggcctac attagttggt
    30241 atacttcctg atgatgagcc acacatcccc tgagcttgtt ttacgttatt cccaaccata
    30301 tctatattca taagtatatc gctacctttt ccaattaaac tagcacctct aactggctct
    30361 tgtatttctc cattttttat taagtaacct tctgaaactg caaaattaaa ctctcctgtt
    30421 acaggattta cagaaccacc acccattttt ttagcataaa gtccatctgg tattgacttt
    30481 ataatatctt ctggtttatc ctctccagca gcaatataag tgttagtcat ccttgaagtt
    30541 ggtgcaaatt tgtagttctg tctacgagaa cttcctgtag gattcattcc cattcgtcta
    30601 ccattaagtt tatctatcat ataagacttc aaaattccat tttcaatcag tatattcttt
    30661 tgagttggat ttccttcatc atctatattc atagaacccc agtagtttgg tattgtacca
    30721 tcatctatag ctgtaatctt tgtggaagca atctgttgac ctaatttatc tgcaaatata
    30781 gagttcccct ttgcaactga tgtagcttcc aaagaatgtc cacaagcttc atgaaatatt
    30841 actccaccaa atccattatc tattgctacc atcatattac ctgcaggaca atttttagca
    30901 tgcaacatag tatgcgctac tctagctgat tcttttgcat aatattctgg gtctatttct
    30961 tcaaatattt caaacccttt acaacctcca ggaccttcaa atccagtttg attttcatta
    31021 cccttagatg caattgaact tatccccagt ctggttctta ttcttctatc ctcaacatat
    31081 ataccttcag tgtttgcaat taatattttt tggtctttat ccaaatagct aacacttact
    31141 tgagatatat cattactaaa ttcttttgca ctcttatatg caaccttcat aacacttact
    31201 ttatctctat ttcctattga atttggataa atttttatat tatgtatgtt atttatttta
    31261 atactgtcat ttaatattac actttttcca tcttccaatt tacctaaagc aacagcagct
    31321 ttgtatgcta catccaacag actatttaat gaattgtcac ttgtataagc atatatactt
    31381 ttcaatcctt taaatattct tattcctata ccataatttc ttccacctat tgcattttct
    31441 actttattat ctattaaact aatagaatta tttatagtgt cttcttcaaa aatctcagca
    31501 aaatctcctc cagtaattag acacttttct aataccttag aagctatatt ttttgaaagc
    31561 ataaatttac ctcctacttt aaatttttaa ttaactttta ttcaatcttt ttacttgtta
    31621 acaaacatta tatactatat tacttaaaat aaggtaatta tgaatatatt atacatatgt
    31681 tttatactaa aatgttatca cattttatct atacataccc atataactag ataattaaaa
    31741 tgtataatat tatttgctat agaaaataat acactcatac ataataaatt ttaggagtga
    31801 agtatatgaa tcaagaatat gtaaaaggaa atcctatttt taccaatatt aataaagttc
    31861 caagacaatt tccatacctt actgatgata ttgatactga tgttataatt gttggtggtg
    31921 gcgtaactgg atgtatttgt gcttactatc tagctaaaaa caatataaag tctgtcattc
    31981 ttgaaaaagg aaggattgca catggaagca ctagcgtgac aacttctcta ttacagtatg
    32041 aattagatga caatttgata gatttaacag aggttatgac tctaaaagat gctctaaaag
    32101 catataacct ttgtacttca gcacttgaag aattagatac atttatagag ctatatggaa
    32161 ataagtgtga ttatgccaaa agagatactc tactttatac tgccaacaaa cttgaagtta
    32221 aggctatcaa agaagaatat aacttaagaa aagaaaatgg atttgatgtt gaatatattg
    32281 atgaatccac aaaccctttt agctttgatt taaaatcagg attgatagca aagaatggag
    32341 gacgagaatt agacccatat aaatatagtc accatttgat tgatgtaagt ttaaaaaatg
    32401 gtcttcaagt atatgaaaat acagaagtaa aaaaagtaga tttatcaaat gataaggtta
    32461 ctgctgaagt atcgtatgga tacaaagttc atggtaaaaa attaattgta gctacagggt
    32521 acaatacaag tttatttaca aaaagaaatt ttgcaactaa gtcaaatacc tttaatatag
    32581 caactaaacc acttaaaaac atagcttctt ggaaaaacaa cattttaatt agagataact
    32641 gtgacccata taattatcta agaactacta aagacaatcg attaattata ggtggtgaag
    32701 atgtaaattt tgatgatata gaaaatgaaa ctttagccaa tgaaaagtac gatattttag
    32761 aacaaagatt gaaaagtatg tttaaggata taaaagacat tgaagttgaa tataaatatt
    32821 gtggttgttt tgcttcaact ttagataatt taggttttat tggtccagat aataaaaata
    32881 acaatctatg gtattgctta ggttatggag caaatggtat actctttgca atattaggag
    32941 gtataatgtt aagtgaatta tatcttggca aacaaaataa aaatatgaaa ttatttaaag
    33001 tagatagatt tgataaatag atattatata ttaaaagact gtaaaaagat gaaactttat
    33061 aattctaaaa gttaactttg atacagtctt ttataatgtt ccttttaaaa atactttatt
    33121 tataattaaa tataaagtaa aatgttaaaa tataactgat aaaatttagt cataagataa
    33181 tatttaagtt tatttgatga aatatggagg aattattatg aaggaaatac tcgttattgc
    33241 tcccactaaa ggaacctatg aaaaatcaat tcacatagtt aaaaaaaaca aatatacaaa
    33301 tattgatgtt gtatttggca atctaaaaga aggtatacct cttgctgaaa aaagtattaa
    33361 tcatggtact agaattatca taagccgagg tggaacttac aatatgttaa aagctactta
    33421 caatatacct atagttgaaa ttaaagtaga tgcttatgat attataaaaa gctacaaaga
    33481 ggtaaaaaat tccaacgaac catttggaat aattggattt aataatgtca tctacggttt
    33541 cgatattata gaagaaatat tgaataagaa aataactatg attgaaattg aaaaagaaga
    33601 agaaatttat gatgctatag aaaagtacag aaagaaaggt ataaacacat atataggaga
    33661 taccactgtt gcacatattg ttaagagact taattgtaag ggaatattaa tcgaatctag
    33721 agaagaaaac atacttagag ccattcaaca agcagaacaa atactcgaag caaccaagga
    33781 tgaacaaaaa agaagattac agatagaagc tatgacagat tttgtacatg atggaataat
    33841 aacagttgat aaaaatttta aaataacatt atttaataag agtgctgaaa aaatatttgg
    33901 tataaaaaaa gaaaatgcac tttatcataa tgttgtagat gttatcccaa acacagttct
    33961 tccacaagta gttgagacta tggaacctca aattggtgaa atacaaaata tagggagtac
    34021 aaaaattatt acaaatagag ttccaatcat tgttgatggt cagattcaag gggctgtttc
    34081 aacatttcaa gatagcaagg agataagttc ttttgaacat acaataagaa ggagcttatt
    34141 aagcaaaggt ctatctgcta aatacacttt tgatgatata attcatgcta gtgaaaagac
    34201 aaaagattgt atagaacttg caaaaaaata tgcttgttat gatactccta tgattattac
    34261 tggagaatct ggagttggta aagaactatt ttgtcaaagt atacataact atagtaagcg
    34321 taaaaatgca ccttttgttg ctgtaaattg tgctgccata cctccatctc taatagagag
    34381 tgagtttttt ggttatgagg aaggttcatt tacaggtgct agaaaaaaag gtaaagcagg
    34441 tgtattcgaa ctagcacatg aaggcacaat cttcctagat gaaataagtg aaatccctct
    34501 tgaacttcaa ggtcgattat tgagggtatt gcaggaaaaa caagttatga gaattggtgg
    34561 tgataaagtt attcctatag atgtaaaaat tatctgtgct tcaaataaag acttaaaaaa
    34621 tatgatgcgc aatggtttat ttagacaaga cctttatttt agaataaata tactaacatt
    34681 atatcttcct tcattaaaag aaagaaaaga agatattata aaattatcag aattttttat
    34741 acataaatat tcaagcaaat acaataaaac gcctttaatt atcactccaa atattaaaaa
    34801 ggcacttctt gaacgagaat atgaaggtaa tataagggaa ttggaaggta tgatggaaag
    34861 ggctgttatt gtggaatcat ttgactcgat tttattagaa aagcttagca atgaatatac
    34921 aaataatata gttgataaag ataatagtga ttttgaatgc agtaatttag atttaaaaac
    34981 tttagaaaaa aaatatatct ataaaattta taacgaaaat aatagaaata ctacaaaaac
    35041 ctgtgaaatt ctaaagataa ctcgctccac tctatggcgt aagttaaaag aaatagaaga
    35101 tgatgtttaa aaataataca tatctatata tatcaaaacg ttttatgttt aattttaata
    35161 cataagacgt ttatttgttt tcaataaaaa atacaattat atgctaaaaa gaatctttgt
    35221 aagtgaaatt ctcgtcaatc atacttttaa ttataagtaa tctacagcat ttcaatcgat
    35281 tattaaaaat aatcatgata actaaagata caaactctat atattggcat agtaattgct
    35341 tttataatac ctatataaaa atgaaaggga gtgatactag atgcacatct taaaatcagt
    35401 gaaaaagata ccaggaggac ttatggtctg tcccttgtta ttgggagcac tatttaatac
    35461 agtatgtcca caagctcttg aaattggagg attcacaaca tccttattta aaactggtgc
    35521 aatgagtata cttgctctat tttgtctatg caatggagct caaatcaata tcagacaagc
    35581 tggtaaacca ttgactaaag gtatagtact tacagcaaca aaatttataa ttggtgcagt
    35641 attaggtatt attgttagta aatttattgg acctaaaggt ataattggta ttacacctct
    35701 tgccatagtt gctaccatga ctaattcaaa tggaggtcta tttgctgctc ttgcaggtga
    35761 atatggagac tccacagatg ttggagcaat atctatctta tctctaaatg atggtccttt
    35821 ttttacaatg cttgcttttg gagtaactgg acttgcaaat gtacctatag tagcactatt
    35881 tgcagcactt ataccaatac tacttggatt tatacttggt aaccttgatg aagatatacg
    35941 agaatttctt gcacctggta caacattatt aattcctttc ttcgcttttc ctttaggagc
    36001 agcattaaac tttaatcaaa ttataactgc tggactacct ggagttgtac ttggtttagg
    36061 agttactata ataacaggta tgggtgggta ctttgtaatg aaacttatac gtgcagaaca
    36121 tccagcagta ggtgcagcta caggtacaac agccggaaat gcagctggta ctcctgaagc
    36181 tttagcagct atagacccta ctctagctac aatagcagct gtttcaacag tacaggttgc
    36241 tgctgctatt attgttacag caatatgttg tccaatgtta gtttcctatc tagataaaag
    36301 agaaaaaaag aaaaaatcta tttttaaaac aaataaaaat attgaagtaa attaatactt
    36361 aaggaggtaa aattaatgaa tgcaattatt catgcaaatg aaaaagataa tctagttaca
    36421 tgtgtcagac ctttagttaa aggtgaaaaa gtttcaattg atggaaaatc ttacatagta
    36481 aatgataata tacctgtctt tcataagatg gctacagaag atgtaaaaaa aggtgatgtt
    36541 gtatacaaat acggtgaaat cataggaatt gcaacagttg acataaaaac tggtgactat
    36601 gttcatgtac ataacattga aagtacacgt ggtcgtggag ataaaaaata ggaggaagtt
    36661 aaaatgaaat taatgggtta tttaagagaa aatggtcagt ttggtattcg taatcatgtt
    36721 ttaattattc caacatctgt atgttcaagt gagacagcta caagaatagc atcacttgtg
    36781 cctggagcaa tagcaatccc tcatcaacat ggatgttgtc aaattggttc tgatatagag
    36841 cttacagcaa agacattaat aggatttgga aaaaatccta atgttgccgc agttttagta
    36901 gttggtcttg gatgtgatgg tattcaagca aaagaattag catctgaaat agctacaaca
    36961 ggaaaaaagg tagattacgt tgttatacaa gaatgtggtg gaacattaaa aacagtttct
    37021 aagggagctg aaatagtaag taaaatggct cgtgaagtgt caaaagaagt aagagttgaa
    37081 tttggtatga gtgaaattac attagcactt gaatgtggtg gttctgaccc aacaagtgga
    37141 atagcttcaa acccttcaat aggcgttgca tcaaaccttc ttgtagatga aggtggaagt
    37201 agcatactaa gtgaaactac agaagtaatt ggtgcagaac atttgttggc tacccgtttt
    37261 gaagatgaag aaatgaaaga taagtttctt aaatttgtaa gtgatgtaga aaaaagagca
    37321 attgctatgg gagaagattt acgtagtggt caacctactc caggcaataa agctggtgga
    37381 ctttcaacaa ttgaagaaaa gtcacttggg tgtatgtata aggcaggcaa taagccattc
    37441 aaaggtgctc ttgaatatgc tgatattgta cctcctgata aaaaaggatt atattttatg
    37501 gatactccag gtcaagatat agattctatc actggtatgg tagcaggtgg tgcacaaatt
    37561 gtaatctttt caactggtag aggtactcca acaggaagcc ctatttcacc agttattaaa
    37621 atcactggga atagtgatac atacaacaaa atgccagaca atatcgatat aaatgcaggt
    37681 aaaattataa cagatggtgc taaaatagca gatataggtc aagaaatatt taatgaaata
    37741 atagaagtct gtaatggtaa acataccaaa gctgaaagtc ttggtcatag agaatttggc
    37801 atttacagaa tttcatctac attttagatt gtaacaaaat atcttttata aatataatac
    37861 ttatgtagat acataagcta aaagttcttt ctaatactat aaaaattttc tatactgaaa
    37921 aggaactgta tcaaaattat tttgaaaaca gttccttttt ttaacctctt ttactcaaaa
    37981 tattataaaa taagatttaa acaataaacg tatcttgatt aatctctaag aagacataaa
    38041 taaagctaaa tgtaaagtgg gataaataaa atttaatatc tttgtatgaa aagatatttg
    38101 gtataatatg tctgagagaa ttcttatttg taaacaaata aaggatggag gatgaaacaa
    38161 aagaatgaac tttgaaaaca caaaaaataa aaaaataata gctactttac tattgacact
    38221 acttttaatc gtgtcattaa catgtgtcac ttttattatg aatctaaata aagtaacaca
    38281 taatgaaaca gaacgatatc ttgtagaagt ttcacaaagt atttccacta caattaatgt
    38341 gaaatgtaaa agtatattta ggacactata ttctatggga gatacttatt ttgagattaa
    38401 tgatagtgaa aaagaagtca atagatactt atcaaaaaaa ttaaaattat ttgaatttga
    38461 ttggttaggt attatagatt ctaatggaaa ttttgtaagt gcccttaaag atacaggtaa
    38521 tgttaaagga caagactttt atagaaaagc ccttcaaggc aaagaaacaa ttactcctgt
    38581 taaatcacaa tctggtacat atggaatttt atattctgta ccactataca atggcgataa
    38641 aattgttgga gctgttgctg cttggaatac tattgatact atgagaaatt tattaagtgt
    38701 agaaagcttt catggtgaag gattttccca aattatcgac caagatggaa attttattgt
    38761 aagttcagca aacaaaaatg ctccaaaaaa tgtggtaaat ttttttgaca tgattaaaca
    38821 ccaaggaaaa caggttgata aaaataaact tgaagaaatg aaaataaact taaaaaagaa
    38881 caaaactggt tctatgtatt atacattaaa tgatggtatt tctaaggcaa tgcactatct
    38941 tccattacaa gaagaaaact tatacctcct ttcaattgtt ccaacaaaaa cagcaagtgc
    39001 acagtttgat ttacttctaa aaaaagcaat cttcatcaat attttaattg ttatactatt
    39061 tttattgctt attatattga ttatgtttat aaatcaaaag aacagggcac aacttataaa
    39121 aatcgcctat gtagactctg taactaaagg ttacagtaaa gcacgttttg atattgaagc
    39181 taaaaaaatt atacagtcat cttcatcaga aagctattca ttggtatcta taaatattca
    39241 gaaattcaaa cttattaatg attcttttgg tagtgaagca ggagataaaa cattaaagta
    39301 catacatgat gtcattagta attatctaga accaggagaa ttgctttgtc gagtttctaa
    39361 tgaccagttt aatgtactta ttaaaactat tcctcaaaaa gaaattctta agtacataga
    39421 aaatatcacc aatgatatta atagcttcaa taaaaatgaa gaatgtaaat attttatagc
    39481 tctatctata ggtgtatata aaattgatga cccaaatcta cctttagttt caattcaaga
    39541 ccgtgcaaat gttgcacgaa aaaataataa aggaaacatt ggaaatcatc cactttatac
    39601 atgtgttttt tatagtgatt tagagcgttt aaaaatgatt aaagaaaaag aaatggaaaa
    39661 tagaatggag gaagcattaa ataataacga atttatagta tatcttcagc ctaaaataag
    39721 cctcaaagat aatactattg taggtgcaga agcattagta agatggcaag acccaaataa
    39781 aggtcttata ccaccagacg agtttattcc tttctttgaa aaaaatggtt ttattacaaa
    39841 actcgaccac tatgtttttg aaaaagtatg tagtatgata agaaaatgga tagataatgg
    39901 agaaactcca atacctgttt cagttaattt ttcacgtgct aatttaaata atatagattt
    39961 tttagaaaaa tacagaatgc ttcgtaataa atacaacatt cctgcaaagc tcttagaaat
    40021 tgaattaact gaaacacttg tatttgaaaa tctccaaaaa ttaattaata taatagacca
    40081 aatccatgaa gaaggttttc aatgttcact agatgacttt ggaagtggat actcttcact
    40141 caatatgtta aaagaaataa aagttgacac attaaaactt gaccgtgctt tttttagctc
    40201 acctaatgca gataatattg ctgaaaatca tgtaatagaa tctatagtta atttggcaaa
    40261 aaaattaaat atgaatagta tatcagaagg tgttgaaaca atcttgcaaa tggattatct
    40321 taaaaaaatt aattgtgata tgatacaagg atatgttttt tcaaaacctc tacctcaaga
    40381 aatatttgag aaaatgactt ttggaaaaaa actagaagaa accagtatgg atagttccgt
    40441 ttaaataaaa taaaaaactt gtctctataa aataatttta atcattttat aaagacaagt
    40501 ctttttattt tccaatttaa ttttattttc taaatttctt acactcggct tgttttaaat
    40561 tcccttttta agaataataa tacgattatc atagcaataa aatctgcaac tggctgtgcc
    40621 aaccaaacac catctaaatt aaattgaatt ggaagtacaa tgattaatgg tataaataaa
    40681 ataaattgtc ttaaaagagt taaaaacatt gaatgtttaa ctttacttat acactgaaaa
    40741 tacacaggtc ctacaataga aatccccatt ataggcaatg taattagatt taggcttata
    40801 cctcttactg ctatatccat caaatctgaa tctttattaa atatacctac aaatacttct
    40861 gggaaaactt gtactactaa aaatccaaat gacaatatga caactgacac tatgatagat
    40921 aatattaaag ttttctttgc acgctcatat tgttttgcac cataattgta tgatattata
    40981 gtttgcatac cttgattcaa tccaaacaca ggcatcataa acatcaataa aatagatgta
    41041 agtgctgtca tagctccaat agctaaatct ccaccataaa cctttagaga attatttgta
    41101 aacatgtgag ttatacttat agctacttcc atagcaaaag gtgtcattcc tattgcaaat
    41161 accttcttta ctatatcttt attcaatttt aagttacatt tttttagttt taaattggat
    41221 ttccctctta taaaataatg tattacccaa aagaatacta ctatttgaca gagtactgtt
    41281 gctatagctg ctccttttat acctaaatta aatacaaata taaatatagg gtccaatatc
    41341 aggtttaata cacatcctac aatcattatc cttgctgcta attttggatt accttctgcc
    41401 ctaattgcat tattaagaga aaatgcaact aaattaaata tacttccaac caatatgaca
    41461 ctcatatagt cttttgcata aaatattgaa tctttacttg cacccaaaat aaaaagtata
    41521 tcttctaaga aaactaatcc taatatagtt ataattaaag agataattat agataatgtc
    41581 aaagcattac ctaatatctt ctctgcttct tctctatttc tttcccctaa ctttatagac
    41641 aatcttgtgc tagcacctac tgataccaac attccaaaag ctattattag agtaaatact
    41701 ggcatagtta caccaagacc tgcaatagct atagagccaa taccttccat agaaccaata
    41761 aaagctctat ctactacatt ataaagtgaa gttaccatca ttgctattat tgcaggtatc
    41821 gaatacctaa ataataaaat ccatattttt tcatgtctta atgcttcttg attctccatg
    41881 ttgtcaccat ttacaccatg tcataagtta tacaaattgg tttatttgtt cttgggtctg
    41941 ttgctatttc tgcatcaata ttaaataatt cttttagatt atccttcgtc ataacatcat
    42001 atgcatcacc ttcacaaact attttacctt tcttaatacc tatcatgtgg tcagcaaatc
    42061 ttgctgcatt attaagttca tgtattacca tgacaatagt tcttccttgt tttttattta
    42121 gttcatctaa taattttagt atctccaatt gatgtgccaa atctaaatat gttgtaggct
    42181 catccagtaa taatatatca gtttcttgtg ccaaagccat agctatccat gccctttgtc
    42241 tttgtcctcc tgataaatct tccatatttc tatccttaaa ctctgcaatt ccagtaactt
    42301 ctaaactcca atttacaatc tctctgtctt ctttactaga attaccaaat ccacttttat
    42361 gaggaaatct tccataagaa attaattctg aaacggtaag accactagga gcttgagggc
    42421 tttgtggaag tacagccata tcctttgcta tttctttaga cttctttttg tagatatcct
    42481 ctccatttat atatattttt ccgctattag ggctaataat tctacctata gttttaagta
    42541 ttgtcgattt tccacaacca ttagccccta tgatagtagt tatttttcct tttggtatat
    42601 ttaaatttaa attttttata atatctgtat ttccatatga tacatttaga ttcttagtat
    42661 ttatacagtt cataacaaca ccctttccta atcatttacc aacattaaat aaataaagta
    42721 cggcactcct actatagaaa ctactatacc cactggtatt tctataggtg ctagaagatt
    42781 tcttgatata gtatctgcta ttaaaagtaa taatgttcct atcaatgctg atactggtat
    42841 taatctttta tgctttggtc ctattatttt tctacctata tgtggagcta ttaaacctaa
    42901 aaatgaaata ctacctgcta ctgctgtcga tacaccagat aaaattactg catatattat
    42961 aagtcttctt ctttgttttt ctacatctac accaagactt atagatactt catcacccaa
    43021 atttaaaaca tctatatatt tagatttata aatagttata cccataaaaa tcactataaa
    43081 tggaagcaca gctattacat atttccaact ggttccccaa atgcttcctg atgtccatgc
    43141 cataacccta ttaaattctt gtgttgtaaa tttcaattgg aatatcgtca aaattgaagt
    43201 aaaagctatg tttacaccta taccaataag tagtaatctt gacgaattta taccaccttt
    43261 ccatgcaaga gaatatataa gaaatgctcc aaataaagct cctataagtg caacaatagg
    43321 cattgtaaat acagtcaagt tactcatacc ttcatataca ttaccattca taaggtatat
    43381 ataaactact acaaacaatg cagcacctga atttattccc aaaattccag aatcagctaa
    43441 atcattcttt gtaactcctt gaagtattac acctgctgtt gataatgcag aaccaaccaa
    43501 aagtgctatt actattcttg gtaatcttaa tttaaatatt gcaatttcat gattacgctg
    43561 accctgccct aaaaaagtct ttataacatc cattggttct atagaaaatg agcccatatt
    43621 taaactcaat aaaaaagtga taaaaataag caccattaaa attgatataa caagtaatgt
    43681 tttagaattt atttttataa tatttttcat agtacctatt tctccccctt tctaacaagg
    43741 tatataaata ttggcactcc tataagtgct gttatagaac ctactggtgt ctcatatgga
    43801 ggatttatca tcctacttaa tatatctgta tatacaagca atacagctcc taatactaaa
    43861 gagctcggta ttatatactt ataatctgaa cctactattc ctttagctat ttgaggaact
    43921 ataagaccta taaaaataat attaccagac actgacacag atgctccagt cattaatatt
    43981 acaagtacta tacatataaa tctaacaaag ttagtttttt gtcctaatcc tattgctact
    44041 tcttctccaa gacttaatat agttattctt ggagccataa taatagctaa aataaatcca
    44101 ataccaccag ctacaaatag caattttata ccttcccact tcactcctac aagccctcct
    44161 gatatccaga aacttaattg ttgagatagg ttaaaataca tagatattgc tgatgccaaa
    44221 gaaattagca atgtaccaag agcgactcct gcaagagcta attttacatt attaactctt
    44281 cctcttgatt tagaacttat aaaatatata aataatccac ttatacttgc tccaataaat
    44341 gcaaacatca ttacactaaa acttccatgt ataagttgag gaagtttttt ttgaagtacc
    44401 agtgatatcg aaattgcgaa tgtagctcct tgtgtaattc ctataacaga tggttcagct
    44461 ataggatttc tcgttattcc ttgcattatt gccccagaaa ctgctaaaaa ccctccaaca
    44521 agtgcagctg ctattgccct tggaagtcta acatccttta ctattctcat atttattcca
    44581 tcttccattt ttatcaaact atctataaca gttgatagat tcatatcttt tgccccaata
    44641 gttatggata atacaattcc aaatacaagt acaactatac ttactaatat caacaaataa
    44701 gttatatttt ttttactcag cttagtcaat tatcctacct ccttttccct tttaatcgtc
    44761 agtataatat catctattaa gtattttaat tttaagattt attaaaaata ggtgtgaaaa
    44821 atgaatatca ttatctcaca cctactaagt taagaattac tttgttaatt catttttaac
    44881 tgattctaat aataactctt ttccaattgg attgtatgat tgactgaaaa atggagcagc
    44941 atcaagtatt gttacattac catcttttac tgctcttatt tgagcccaga tagcactatt
    45001 ttctaaatct tttttatctg cttctgtagc aattacaact atatggtctg catctatatc
    45061 tgttaaacct tccatactta ccattggtag tgttattcca tcctgttttg gcatattctt
    45121 aggtctagct aatttcatat catcattgat tagagtacct attccaccat cactaaatac
    45181 cataaattga cctgaacttg ccaacactgg taaataagtt ttttctccat tcttttctat
    45241 aacttcctta cctaactttg tagctttttc atcatacttc tgtagccaac ttttagcttc
    45301 ttcttctttg tcaaataact ttgaaacatc agttaacttg cttctccagt catttgcata
    45361 atctttcatc ataactacag gagctatttc ttttaattgg tcatatattt tttcttgtct
    45421 ttgactcatt attattaaat caggatttac ttctaatata gcttccatat ccatagtatc
    45481 catcattgag tgcccaacta tctttacacc tttaagctct tctcttatgt aacttggtaa
    45541 tttatcagtt tcatatgagt ctacatttgc tgttgcaaca ggcttataac ctgctaataa
    45601 tagttcttca ctacttcctg atatatcaac tattttttta ggatttgatg gtatcttaac
    45661 ctctccttta acagattgta caactcttgt ctctgattta tctttatcct ctgtattctt
    45721 atctgaacat gctgtaacaa gtactaacgt agttataata gatataaata ttgcaagtga
    45781 ttttattttt ttcatgatta cttctcctac ttgtttatta tacttacatg atattgatta
    45841 tcataagcac atatttatta tagcattttt ctatttttag tcaatattat tttgtaaaag
    45901 gataaaaatt cctaattaat cttaattata tatagcttag ataaggtttt cttatattaa
    45961 acagtatttt ttttaagaat tggacacaaa tacaatgact tttcagttaa acaatgataa
    46021 aatgcaattt atagataagg aatttagtag ggaggattca aaacatggta catttagtat
    46081 attgtgataa tgcaggaaaa aaaggagaaa aggtcttaga taaaatcttg gctggaacaa
    46141 aaacaatggt tattagaggt gctgctggta gaaaaatacc acatagtaga gtatttgaag
    46201 atgagttact ttattttatg gaaaaaggaa ctgcaaaaat atctgcaaaa gccactgtta
    46261 aaaaggtaga aaactatgtt aaattaacag aagatgaaat ttctaaaatt ttaaatgaaa
    46321 atcaaccaaa attaaattta tctgaaaagc aaaaagttcg ttggcataaa aaatgtttat
    46381 gcttagttga atttgaaaat gttgaagaaa taacacctct tgattttgac catcaaggaa
    46441 atatggatga ttggcttatt gttgaaaaaa ttgaagatgt agttgttggt actagtatac
    46501 cttacaatta tgagaagtct aaattttaaa gtatcaataa cataatacta tattgctaaa
    46561 ctctacttaa ataaaaaaag agtgcattta ataattataa aaattatttt tatacactct
    46621 tttttaataa taaatttaaa ttacttactt tctaatcttt catatacatc tataaattct
    46681 tctgctaatt gttgaaaatt catagctgtc attaaatggt cttgtgcatg aataagtata
    46741 acacttattt cagttttatt accattagct tcattttgta tcaattctgt ttggaattta
    46801 tgagctttat gaattgattc tttcgacttt tcaatacatt ctctagcttt atctatattc
    46861 ccgctctttg cttcttttat tgcttcaaat gctagccctt tcgcatcacc tgcaaatcca
    46921 attattccaa aagatatctc tatcatcttc tcatccattt attcgacctc ctaaatatct
    46981 ctatagcttg atatgactaa tccattcttt tctaaaaatt ctttgacttt tttagatgta
    47041 agtatcttgt gctctttcgt tctatcaata gcataagatg tagaatttaa aatataatca
    47101 tctaaaaaag ctgggtgaga cataatatcc actacatcat acttcattat ttcgtctata
    47161 ttttttataa aatattcttc acttacattt tccttataaa aactatctat taaaggaact
    47221 actttagaat actctaaatt atattcaaat ccacctctta ttggaagatt atacttggtt
    47281 acaatcttct ctattactga ctttaaactt actaaagtat ggatatgatg gtgactatct
    47341 aaatgagata tatctattcc aagccctttt accctatcta tttgagcaca aagttctcta
    47401 taaatctcat cacaatccat tttttcaata atttcgtctg taatcctcct aataaaaaaa
    47461 ccgtctttat caacaatagt ttttaagtta gataataatg gtctaccaca acttaaagtc
    47521 atatgaactc cacaatttaa agttttattt tctttaagta agcctactcc atgttctacc
    47581 ccaggcatat ttgccatcat agaagtagac ctaactatac cattattatg agctgaaatt
    47641 attccatagt tcacagcttc acaatatcca aaatcatctg catttattat aattttagtc
    47701 ataccataac ctactttcta tagacaacta tctattaaat ctaggaagat aatctttatg
    47761 agcttccaac atttcgtcta atatagtttt tgctaagtca tcagatggaa tcaatgggtt
    47821 tatacaaagt gccaaaagag cagattcata atcgccatat acagctgctt tagctgccaa
    47881 tacttcaaat gatttgattt gacttactaa tccatgcaca gaatctggta aatatcctat
    47941 acttattggt tttgggccat tttttgttat tacactactt acttctaccg cttgttcatc
    48001 tttaaaatct cttatagaac ccttatttaa tgtattaact acttggatat cttttttgtc
    48061 gttgtatata gaacttatta aattacaagc agcatcacta tagtaagccc ctcctctttt
    48121 ttcaagttga ggtggtttta catctaattt ttcatcctta tataattcaa ataattgttc
    48181 ttccaattct ttaactactt gacctctagc ttttccctct ttaaactctc tcaactcatc
    48241 ctcaagcatt tctttagttt tatagtagta tctatggtat ggacaaggta ttgcacccaa
    48301 agattttaca aattcagcat taaaatcaat tgcctttata ttttgcatac ttatatctgc
    48361 tttgacaaat ttatcaattg cttcttttgt aacatcttct ccatctaaag atacatttag
    48421 accataaacc atatgattaa gacctgcaaa atccatactt attctgtcac tttctacatt
    48481 aaatagtttg gcaacatcat tttttaaatg aattggcacg ttgcatagac ctatatatct
    48541 tctaaaatta gtatatttga atacagcctc tgttattatt cctgttggat ttgtaaaatt
    48601 caccatccat gcatttggac ataattcttc cacatctttt attatatcaa atataactgg
    48661 tattgtgcga agggctttaa ataaacctcc agcaccatta gtttcctgcc ccataactcc
    48721 atgagataaa ggaatagttt catcttttat tctcgcatct aaaagaccta ctctaagttg
    48781 tgttgtcaca aagtctgcat ctcttagagc ttctcttcta tctaaagtaa gatttatttt
    48841 catatctact ccagcttttt ttaccatcct cttagctaga tttccaacta tctctaattt
    48901 ttcttttcca gcttctatat ctacaagcca taattccttt acaggtaact cttcatatct
    48961 atttataaaa ccctctacaa gctcaggagt ataacttgaa cctcctccaa ttgtaactat
    49021 ttttagtccc tttttcatct cattatacca tccttaccta taatttaata tactctacaa
    49081 aaccctatac atttatttac cgaaataaaa ctacctagtt gttaattttc aatttattag
    49141 cttccatttt ttcagacgct atgacaaatg gaatatatat taatattgat aatattaaat
    49201 taaatagtgc taaagctccg ccagtccagt cacctgtagc cattgctcca cctaatatag
    49261 gaggtgttac ccaaggtatt ttaggtaaaa ttactggtgc tactaatcca aaatctattg
    49321 ctaagtatga tacagcacta catattactg gtgcaagtac aaatggaatc ataaatatag
    49381 gatttaaaac tattggaagt ccaaatatca atggttctcc tatgttaaat attgcaggtg
    49441 gaccacctaa tactatcatc tctttacgtc ttcttccagc tattaataaa gctactatta
    49501 atcccaatac cattccagaa ccacctaaat atacaaatgc atcaaagaat gcacctgcaa
    49561 gtacatggta tccttccgtt gctcctgctt gagccaaggc agcattttct ccacctaatt
    49621 tcataagtat agtttctgta aaaggaagtg ctatatttgc accatgaagt ccaacagtcc
    49681 aaagtatatg aattataaat gcaataagca ttgtagaacc tagactatca gctacatttg
    49741 atagcggtgc tcctaaataa gtatttaaaa tatctgttaa gaaacttcca ttagaaagca
    49801 tttttataaa tattcctatt acagtaaata tcattatagt taacattcct ggtattaact
    49861 ttgcaaatga tcttgaaact gctggtggaa ctccatctgg catttttata gttaatttct
    49921 ttaccttaga taatcttaca aatatctcag ttgataataa tcctattaat atagaagaga
    49981 ataaagctgc tgtacctaag taggtttgtt gtatcattcc ccatccttca actactgtag
    50041 ttcctccctc aggaactatt ttaccaattt gtggagccat tataaaaaag atagataaag
    50101 ctataagccc agattgtaac ccatcactct cataagattt tgctagaaaa taagcaacac
    50161 ctattactaa gaatactgcc attgtaccta ttgctcccca ccaaaggtcg cctccaaatg
    50221 tgctccagtt ttctccaaat atacttgcca tcaaattttt gtatgcttcc aatggtaagt
    50281 tatttatcaa tgttcccaat gcacctacca tagttatagg tatcattact ataaatgcat
    50341 ctcttactgc tactaagtgc ctttgagctc ctattttagc agctacaggc actatatatt
    50401 tatccataaa tgacatgaat ttttccattt tacatcctcc agtacaactt tattttgaac
    50461 tatttaactc tacagctcta tctaacacag cttttccatt acatcttcca taatccatca
    50521 tatttataac ttctactgga atattatatg gttttgccat ttcagttgct tttcctaata
    50581 catatctaac ttgaggccct aaaagtaaga catcacaatt ttctatttca ttttttaagt
    50641 tcacttctga tatagcccat atttttgctt ctataccgtt ttcctttgct gcatcctcca
    50701 tttttgtaac taataaacta gttgacattc ctgctgaaca agctaacatt atttttatca
    50761 ttttttcccc tccaaattag attcattaaa tattttttaa gtcataagtt ttttaaacta
    50821 tacatttaaa atatgctttt ctattaattt tttcttaatg tatatatctt atttttaaa
//
LOCUS       DAEORZ010000008        49040 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEORZ010000008 DAEORZ010000000
VERSION     DAEORZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.008"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(45..434)
                     /locus_tag="KRH39_000406"
                     /pseudo
     CDS             complement(45..434)
                     /locus_tag="KRH39_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
     gene            complement(540..1703)
                     /locus_tag="KRH39_000407"
     CDS             complement(540..1703)
                     /locus_tag="KRH39_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG8510336.1"
                     /translation="MKGGVRKRGKKWYYYFDAGIVDGKRKKVEKVGGETKKEAEKALR
                     DAINEYENAGIVFDETNMNLSNYLDFWYKEYVLLNCKYNTQVNYRNLIKNHIEPELGK
                     YKLKSINPAIIQEFLNNKTKTYTQNGEERRYTRGNLKAIYGVLNSALKSAVYPYKLIK
                     ENPAQYTNIPKNIIDTKKDSENKTITLDEFNKILEIYPKNTNIYIPLLIGFHTGMRKG
                     EILGLCWDNVDLDNNIIKVRKNLIKRKVSEFELTSPKTKTSIRDIRIGDTLSKILKEE
                     KLNQKKQKIKLGKWYTETEHDWVCRKKDGSFVNHNNIDACVRTINKKLNLDFNFHCLR
                     HTHATLLLENGANIKYIQQRLGHSQLSTTMDTYSHVTSKMESETIDILENLVR"
     gene            1996..2889
                     /locus_tag="KRH39_000408"
                     /pseudo
     CDS             1996..2889
                     /locus_tag="KRH39_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
     gene            complement(3660..3896)
                     /locus_tag="KRH39_000409"
     CDS             complement(3660..3896)
                     /locus_tag="KRH39_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8510337.1"
                     /translation="MLRQLRKMRHLTQLELAEKINYNKSYISKLEKGNYKNVTVTTIV
                     DLAIGLEVNYLVIAAIFIMEELKRRKEKNRLAKH"
     gene            complement(4357..4707)
                     /locus_tag="KRH39_000410"
     CDS             complement(4357..4707)
                     /locus_tag="KRH39_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8510338.1"
                     /translation="MTRSDIMFSNRLRELRKQKGLTQMELAKLLNCSLSKIAMLETDK
                     RDPVKEDLLRFSEIFEVSIDYLLGKNNLDLNPRLNKEMEQALHKLYSLDEENRKAIEK
                     IIDNAYYKIINEEK"
     gene            4851..5039
                     /locus_tag="KRH39_000411"
     CDS             4851..5039
                     /locus_tag="KRH39_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009587961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8510339.1"
                     /translation="MKELSKFRKKLGLTQKQLAERIGISKSYYSKIEGNFKKPGRGFL
                     EKFKNTFPNEDMNIFFKQ"
     gene            5073..5867
                     /locus_tag="KRH39_000412"
     CDS             5073..5867
                     /locus_tag="KRH39_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBG8510340.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETDVNNIQTNFNRNKERFKE
                     NVHYFLLQGEYLKEFKNQPTNSQLVSKHSSQLYLWTERGANRHCKILDTDKAWEQFDN
                     LEETYFKVKQQKPSCIEDVLIESLKEMKDLRLQVNQANNIALEAKTEVKTIKEVVSLN
                     ATDWRKDTQQLIAKIAKKQGGFEHINLLRRESYELLNNRFGVDLNRRLINKRRKMAEE
                     GVSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            5926..6063
                     /locus_tag="KRH39_000413"
     CDS             5926..6063
                     /locus_tag="KRH39_000413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510341.1"
                     /translation="MQREQTTLRIPEDLHKALIDLSSDIGMPITSIIIIACWLYISKI
                     N"
     gene            complement(6049..6360)
                     /locus_tag="KRH39_000414"
     CDS             complement(6049..6360)
                     /locus_tag="KRH39_000414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510342.1"
                     /translation="MTTITVRIYTPLNENLEKISYQTGILKSSLILYAINDIIRNSKV
                     NELQSISYKSDDTVRSTLRIPGVLKELLEKTAKENNLSINSLINNAVHSFCISDWLIY
                     L"
     gene            6481..6651
                     /locus_tag="KRH39_000415"
     CDS             6481..6651
                     /locus_tag="KRH39_000415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510343.1"
                     /translation="MEKVRMTVRLVPKLNQYVQNIAKECGKSKNSIIVDACWEFIEKI
                     KKENKFNVESEE"
     gene            6654..7292
                     /locus_tag="KRH39_000416"
     CDS             6654..7292
                     /locus_tag="KRH39_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510344.1"
                     /translation="MNNLQIFEKLEFGQIRMAEIDNKPYFVGKDVAKSLGYKNTNDAI
                     LRHCKGVVKHEGFKINGIKIALITEGDVYRLIVGSNLPNAEKFESWVFDEVLPTIRQT
                     GQYQAQQHVITELTGTIGDLKGTIKEYKKLCKITCSKKQQYSKYIKNRLDIDKANKEY
                     NQVKARLFLILGVEKWEDIDFDTSNNLIQIIDDCIKVIKSERPYKQLSYFEN"
     gene            7351..7545
                     /locus_tag="KRH39_000417"
     CDS             7351..7545
                     /locus_tag="KRH39_000417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510345.1"
                     /translation="MKNNTSDSRVKYFCKCPYCGFDNEVEVKKGLKPKTCCICTKEVE
                     YEKLEQQSASENTEIKGVCN"
     gene            7546..7743
                     /locus_tag="KRH39_000418"
     CDS             7546..7743
                     /locus_tag="KRH39_000418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510346.1"
                     /translation="MKISFESVVNNVNNETELILSKEELQIAKRILNTLNENERSILS
                     SKDILDFCKEALKYNLVPKFI"
     gene            complement(7736..7930)
                     /locus_tag="KRH39_000419"
     CDS             complement(7736..7930)
                     /locus_tag="KRH39_000419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510347.1"
                     /translation="MKVGDIMQFSKDILHTLTLEILKEKYDFKNSSEEELLKHYHEIF
                     LKLSEVNNSFSKGDGLGVFK"
     gene            7983..8180
                     /locus_tag="KRH39_000420"
     CDS             7983..8180
                     /locus_tag="KRH39_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8510348.1"
                     /translation="MAINDNINKILKDKNLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKIADYLDVTLDELVGR"
     gene            8210..8653
                     /locus_tag="KRH39_000421"
     CDS             8210..8653
                     /locus_tag="KRH39_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8510349.1"
                     /translation="MSKLGNISNFNLDKQEDKSFNDLDNISICFSEGIRKVVEMNLNN
                     YKNKISKYLNETSKIELLEPKELTIVISKGYPDYLMSVEEASKRLKIDKVFGYELIKN
                     GLLKSVDIGATKVSSYELDDFITRNQGKNIKEMLREMKEIREGVI"
     gene            8781..8969
                     /locus_tag="KRH39_000422"
     CDS             8781..8969
                     /locus_tag="KRH39_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510350.1"
                     /translation="MKIIYKNKVYKLEQDKVLFRITYYDEQRGSKKFNKDKKVKRSVL
                     TRDIEVVNLYLPTHLKIK"
     gene            9001..9141
                     /locus_tag="KRH39_000423"
     CDS             9001..9141
                     /locus_tag="KRH39_000423"
                     /inference="COORDINATES: protein motif:HMM:NF015807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBG8510351.1"
                     /translation="MQREQTTIRLPKELKEKLEKQASKKGRSFNSILLSILQEFIQNP
                     NV"
     gene            complement(9119..9307)
                     /locus_tag="KRH39_000424"
     CDS             complement(9119..9307)
                     /locus_tag="KRH39_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBG8510352.1"
                     /translation="MAKMGTYKNPHFAIRIPKEKLDKLKYIAEYNARSANKEIEFLVT
                     KHIEMFEKEHGTIHLDSE"
     gene            9463..9621
                     /locus_tag="KRH39_000425"
     CDS             9463..9621
                     /locus_tag="KRH39_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510353.1"
                     /translation="MLANRVRTGLRIPYDLNTKLILIAEERGMSKNSLILHILWDYIK
                     EIENKEVE"
     gene            9621..9848
                     /locus_tag="KRH39_000426"
     CDS             9621..9848
                     /locus_tag="KRH39_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510354.1"
                     /translation="MVESTKEFDLQTIKAGNAVKVNCKRFGFEIDCIVVVATEKELNL
                     AYFDEGRGCMEYQALITEDIQDGDYEIKILS"
     gene            10043..10933
                     /locus_tag="KRH39_000427"
     CDS             10043..10933
                     /locus_tag="KRH39_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBG8510355.1"
                     /translation="MDWLKNRQLGIGGSDASAVAGLNPWKTSVQVYIEKKEEIPIETK
                     SFRMELGNRLEGLVAELFTEETGLKVRNVNGMLKNEKYPFAIANIDRAIVGEKAFLEC
                     KTTNSFSIKEWENGVPLHYEIQCLHYMAVTGATHCYIAALLGNEKFVWHKINRDNEVI
                     KNLMKIESEFWEENVLKDILPIPDGSDAYSEFLKTRYKNSVKEKIELNLLEDGISKLK
                     RYDDIVLQMKELKGEKQLIEQEIQSEMREFELATLGGRIITWKGATKRSIDTKRLREE
                     MPDIAEKYTNISSYRTFKIK"
     gene            10946..11779
                     /locus_tag="KRH39_000428"
     CDS             10946..11779
                     /locus_tag="KRH39_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBG8510356.1"
                     /translation="MASEKAKGALEKKVSGANTVKVSPSKGMEQLMNKMASQIKKALP
                     SMVSSERFQRVALTAFSNNPRLQSCEPMSFIAAMMESAQLGLEPNTPLGQAYLIPYGN
                     KVQFQIGYKGLLELAQRSGKIKTIYAHKIRENDKFEIKYGLHQDLVHEPKLNGDRGEI
                     IGYYAVYHLDTGGHSFSFMTKEEIIEFAKSKSKSYSSGPWQTDFDSMAKKTVIKQLLK
                     YAPLSIELQKAMVGDETIKSEIDEDMSMVVDESESLEVDFEVKENMDGKVSVEESINV
                     D"
     gene            11794..12696
                     /locus_tag="KRH39_000429"
     CDS             11794..12696
                     /locus_tag="KRH39_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8510357.1"
                     /translation="MKEDKSVLEKLNILSGGYGLMPRIIARDRWLTVGARMLYSYLTS
                     FAGNDGTCFPSRDLICYELDISKDTFTKYKKELEMSGYIRIHKNKSKQGKMQNNIYEI
                     VFDRTYIEECISKRGLKVDKKKKKPCPKNIDMEPCPTFPDTTQPDTENMDTNSNSINS
                     NSINNMYIGKKPVDNFLKEFKKLYEENIGVIYPVTAEWLLEVSNEVDIRVFKRAIEIC
                     AEKMNMNLAYLKGILKKWKDANITTYEQLESYRLQQESKKSKKVVNNQLSKNKFANFE
                     QTFTKYTEDELDNIIKKSQDAKFK"
     gene            12763..13161
                     /locus_tag="KRH39_000430"
     CDS             12763..13161
                     /locus_tag="KRH39_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510358.1"
                     /translation="MELKFREWNKNGKEMYSYDEMVCYSKNLLREWVYSGVYLPTSNE
                     NFEVMIYTGLKDCDGKEIYEGDIVLCRSIFFTEFAGEVKFKDGCFIAVNEMSGDCFRL
                     SELAIVKVDGNIYEDLSKLNDIRRCDNEGC"
     gene            13164..13415
                     /locus_tag="KRH39_000431"
     CDS             13164..13415
                     /locus_tag="KRH39_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510359.1"
                     /translation="MIIIRSQDRLYLMRVNRVEISNSEVYAMLENDVRKIGEYESNKR
                     AMQVLNEIQKFIENGVRTDYIDSYRIRHNQEEVFEMPFE"
     gene            13434..13790
                     /locus_tag="KRH39_000432"
     CDS             13434..13790
                     /locus_tag="KRH39_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510360.1"
                     /translation="MAKVWVDAGTFLERTEDIEDMFELNLRKVRDRNKKVNVIDLLVN
                     DECQGRKGKKVECFNIVTGETKVFDSVVEASKYVYFTDVYIAHLARTGKVSKNGWKAR
                     YIKEVSNGISKCGASN"
     gene            13759..13911
                     /locus_tag="KRH39_000433"
     CDS             13759..13911
                     /locus_tag="KRH39_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510361.1"
                     /translation="MVLANVEQVIKLAEKILSKKKCSVNKAIDIAIKILSKYECEGIL
                     KIKLGK"
     gene            13934..14128
                     /locus_tag="KRH39_000434"
     CDS             13934..14128
                     /locus_tag="KRH39_000434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510362.1"
                     /translation="MLYLARIQFKREENSKVESGYMIGDGFTNSCFLDEDFNPLSKDK
                     DGFSLYDYRLDFKNPLELKL"
     gene            14160..14333
                     /locus_tag="KRH39_000435"
     CDS             14160..14333
                     /locus_tag="KRH39_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HBG8510363.1"
                     /translation="MKLKDIIKLGEKYCYCPHCGNDKIGNNEGKLIVEEHTYYRECSC
                     GFNILIDDRKDEI"
     gene            14333..14572
                     /locus_tag="KRH39_000436"
     CDS             14333..14572
                     /locus_tag="KRH39_000436"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510364.1"
                     /translation="MHISTTILLLIGSFIAGRVYENHLRTENCEECESIREFIQSNEL
                     CEYCSKEIDTCDKKCYKNILKRRRKYYIDGEDVEK"
     gene            14687..14851
                     /locus_tag="KRH39_000437"
     CDS             14687..14851
                     /locus_tag="KRH39_000437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510365.1"
                     /translation="MTNFEMIKSLDKDGMADFLGCADCICEYCVYEIETCVYKCFDGY
                     KKWLDMEVEL"
     gene            15019..15228
                     /locus_tag="KRH39_000438"
     CDS             15019..15228
                     /locus_tag="KRH39_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510366.1"
                     /translation="MYEYILRWQIGLSLENRKIHYTYGSKDVLREKAKVLAKDENIVL
                     ITIDKVDEVIKNTISEKIIERFENL"
     gene            15240..16244
                     /locus_tag="KRH39_000439"
     CDS             15240..16244
                     /locus_tag="KRH39_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG8510367.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVNQDMDLFFQKKISKVSR
                     DNDIRTAVFNNYSNNLIKKCCEQIIYDESSFLNSSKEIAAYLFDVMKLNATLESCDLA
                     ICLYSQKDEKKVAILKLDYNNSYTHSIEFKDDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNNEYHLNVLDKDAEKEEANSKFVTEFLNATKIKDDKYKTKMFKSTAENWITNAL
                     GNDIKQAEDVRSILNYTLKEKHEIDINDFVDKSIKDDELKNSFKEHIEEKGLDKSFNI
                     DKKWVEKKLKTRNIKTDNGFDIKGNLTDFEDPMKYTVRQNQNGSIDIIIKNVTFYEEK
                     "
     gene            16254..16973
                     /locus_tag="KRH39_000440"
     CDS             16254..16973
                     /locus_tag="KRH39_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HBG8510368.1"
                     /translation="MKYIASFSGGKDSAAMLLLILEKKLPLDEIVFIDTGLEFKEIYD
                     IIDDFEKRINFKITRIKAEKTFEEYFYTVNQQGKRKGQIWGFPYTLGAWCNSRLKLAP
                     ANKYFNKLGEHKRYIGIAFDEPSRYKRLEKNCIAPLFEEKMTEKDCLKYLKEKGFYYE
                     IHHRFKRTGCYLCPKQSLESLKTLRKYYPILWQKMLKFDRDSPVPFRANGVTVNDLEK
                     RFSNEDIENERQISFFSKGVV"
     gene            16976..17368
                     /locus_tag="KRH39_000441"
     CDS             16976..17368
                     /locus_tag="KRH39_000441"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510369.1"
                     /translation="MDLPDNVIEIDILRINRNNDKKCKCKNREFIVDTVNKSVHCSEC
                     GFRVEPYDALYELAMNFERVNGQVNTLLKQRKEIASYKPHLIVFKRLESKYKGKKMLP
                     VCPICGEAFYFENLTSWINKDFYERRKK"
     gene            17446..17715
                     /locus_tag="KRH39_000442"
     CDS             17446..17715
                     /locus_tag="KRH39_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510370.1"
                     /translation="MITTQMYKINNEIFLNNICLEENKEITIWVEKNNTHELIWLKIA
                     NVNGKLAIFVQDIEDAIVNEWQGRIAYERVISQIEDEQVEKGEID"
     gene            17738..17968
                     /locus_tag="KRH39_000443"
     CDS             17738..17968
                     /locus_tag="KRH39_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBG8510371.1"
                     /translation="MIHELKILPQYFKEVANGNKNFEVRKNDRGFKKGDLLVLQEFDG
                     EKYTGLETRKEITYVLENSDYLQNGYVVLGIK"
     gene            18122..18556
                     /locus_tag="KRH39_000444"
     CDS             18122..18556
                     /locus_tag="KRH39_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510372.1"
                     /translation="MQKENKDVWLYSWDGECFGSDEYESKEEAIEAAKEELLNFREFG
                     EWVYDKEFGKLVYVGKREDVSLPGIDVEDTLERVQELIDDEFEDYGEDWITRIKDEDR
                     KILDNKLNEVFCLWVDKFGYKPTWFKVVEVEKIKLNEVANES"
     gene            18546..18953
                     /locus_tag="KRH39_000445"
     CDS             18546..18953
                     /locus_tag="KRH39_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG8510373.1"
                     /translation="MKVNFTIDGEPVGKERPRMNSITKRTYTPNKTRDYEELIRWLYQ
                     SKVKYYFEGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVVKIIADSLN
                     EIAYKDDTQIVEVVASKYYSDKPRVEVILEDIS"
     gene            19016..19756
                     /locus_tag="KRH39_000446"
     CDS             19016..19756
                     /locus_tag="KRH39_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HBG8510374.1"
                     /translation="MNDLKLIENEGLIKVYTTDEDIKVVDGRELWEGLEVEKDFSDWI
                     KSNLENVDAVEGIDFSTLKGKTSEQGGRPSIEYILNLEIAKEICLVAGASPRANKELK
                     RNSKNYRKYLIAVEEKYKVSNNLTKTQLNQINDIVSNALCEMQTKHDAQIEQFKKESS
                     QYYRPTSKTKYDISSYIKDRLDIPRANEEFDLVKKRTLLILGADKWEDIPKDVLLKSL
                     NIIDESIRIIKSERKTNQISFFEKDNFC"
     gene            19868..20350
                     /locus_tag="KRH39_000447"
     CDS             19868..20350
                     /locus_tag="KRH39_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510375.1"
                     /translation="MNKKTLFQEVEGRLYNYKKLESQIRIKDIYIKKLESEFCGCKAQ
                     SYEEKTGPTYNISSSVENEVIKREEDLNRLKEDKKTLEIEKETIECALTSLNSFETEF
                     FNEMYMNNEKINMDYMSNAMHIDRSHCFRIRKRIVCKIMDMLYPKIKEFELPIFSWKA
                     "
     gene            20795..20992
                     /locus_tag="KRH39_000448"
     CDS             20795..20992
                     /locus_tag="KRH39_000448"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510376.1"
                     /translation="MSNLREKIIKELDEFNIDADDEFLSYGVEYVESFTGKSAISEEL
                     LIRGVVFMANYIVNMENKNLD"
     gene            21138..21839
                     /locus_tag="KRH39_000449"
     CDS             21138..21839
                     /locus_tag="KRH39_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBG8510377.1"
                     /translation="MAKARSPNRNKAFDIFKEHNGNIANREIAEILNENEKTISNWKC
                     RDNWNVVLQKDKCSTTKKKGGQKNNKNALKDGIYENIFFNTLTTEEIELIKSENKDEI
                     HRLELEISLLTVREHRHMKRIKQYEEKVDKITLESIDKRTLKIEGNLLKDNEQTQTET
                     VTRAVSIFERVQKLETELTKIQKEKAKNIERLQKLKMDKKRFELEIDNNNKDDSKTWV
                     EAIQNIAMKRGVNNE"
     gene            21832..23241
                     /locus_tag="KRH39_000450"
     CDS             21832..23241
                     /locus_tag="KRH39_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HBG8510378.1"
                     /translation="MNKALLTLLDCYWDNPVWFAEDMLNFKADKWQSDVLMALAQNPK
                     VSIRSGQGVGKTGLESIATVWYLSTRPFPKVVATAPTRQQLYDVLWAEIAKWLSNSKV
                     EKLLEWTKTKVYMKGFEERWWATARTAVKPENMQGFHEDYMLFVVDEASGVADPIMEA
                     ILGTLSGAENKLLLCGNPTRTSGTFYDSHNRDRDLYKTFKVSSLDSPRTSKDNIEMLK
                     RKYHEGSDPWRVRVLGEFPKGESDSLISLEAVETSTIREVNISNDYILNIGADIARYG
                     DDETIIAPRIGGKVFDLLTYSKKDTMETVGNILRAVDKFKNMYHQINRVKIKTDDDGL
                     GAGVTDRLKEVIRHERLKYEVIPIQNGSSAIEKDKYYNKASEMWDNMREELDANLSSF
                     IQNKEAIIQLPNDDKLIKQLSNRKYTVDSKGKIQIESKKEMKKRIGESPDRADAVIYS
                     FAENNNTDLSLLKGGSVWG"
     gene            23232..24734
                     /locus_tag="KRH39_000451"
     CDS             23232..24734
                     /locus_tag="KRH39_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG8510379.1"
                     /translation="MGIISYVKKLFKGPAGEIMRMSSGNIGVYKLDDSRVDYELAREL
                     YQNKNANYKLGSSFVRPIVNSTTGFMGVPHFQIEDEEAQYILDEFVLDNTSKMLKTHT
                     DSLKQGDCYIWITREERENPLYPDKKVRLIYNFISPEEVKEIILDPTTKEPIAYILES
                     QNEWTDLGENKRRAKVKQIITAESRFVEVEGDKIEGLEEGETPNVWGFIPIIHFKNEA
                     DETLKYGQSDIEPIEPLLKAYHDVMLHALKGSKMHSTPKLKLKLTDVASFLAHNFGVE
                     DPVKFAKEGGKINLDGHEILFLNKDEEAEFVEVKSAIGDAKELLKLLFYCIVDVSETP
                     EFIFGVHTPSALASVKEQMPIMVNKIRRKREQFTNSWQLLARMVLIMSSNSSGMKYST
                     YDVNIGWDEVNPRDDKELAETLEKVCSALDKALEGGFISEESTVNFLAQYIDTMSNYI
                     SDDPEIVGEREKIIKTKMLKYRLDDSQGLDDESNEIDKEIDKIKDNNDNG"
     gene            24721..25734
                     /locus_tag="KRH39_000452"
     CDS             24721..25734
                     /locus_tag="KRH39_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510380.1"
                     /translation="MTMDKSTLELITVAGEYKKWALEARKKLIDLRLKQDDEIRTMYI
                     NITRNITKEIRKGNLSDFNKVRLKQILKQLTQEIKILNEQLVFNFDEYLNKNVETATS
                     YSKNILINAVETAQITKVTKAMIQKAFYDINIRTVEAYYTRVKDGLFLSDRIWSKCKK
                     YREDMKVILQTAVTEGQDCVKTAKMLDKYVLKGKKTLVDEYPNMIKRIGNRVPQNISY
                     EALRLARTEMTSAYGDGVLASAMINPATIGIQFMLSMAHPHTDICDEICGEDNFGLGK
                     GVYPINEAPVYPFHPHCLCIMLTVVQPLDILVGRLKNWIKNPMNDVPLEMWYQEVYGN
                     LNF"
     gene            25754..27088
                     /locus_tag="KRH39_000453"
     CDS             25754..27088
                     /locus_tag="KRH39_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510381.1"
                     /translation="MNVITGEMDSMNALISSIKPSDIPLAKDIDIEALKSIDDDPLEV
                     VVEIPATKSKRGWNYTAKSLKDIVDYTNENTLNGFLGHQKAENISTEFAPPVTHWIGA
                     EMKGDKAYFRGLIDADATNLKRWIRTKRIKEVSIFGYPKLKKSAKGEMNVIGYEPLSI
                     DWTPLHRPGMPTSIVGMEMSPNGEQLDGTFEALRIDLREALKAKFSINDNNSYLYIQN
                     IRYDNNTVIYELEQNGLCKLYSIPFTIVENKINLGEEIEVIKKISYEAKGEMKGEENK
                     LEGKELIKNVKGLLQTGEISYSEVIQGIGLTKEIVTGEMEDVKSSLKAEKELREVKKV
                     LGIVGEMDTVEVAKKASKALENEKKEAWNCIVNKVIKDKVSGEIAQTLVKKMLNVEEG
                     SSEEVITGEIENILNDEFVKNTMSNMYKDNPTTTGLLNSSNNGSLTTKKNRI"
     gene            27104..27487
                     /locus_tag="KRH39_000454"
     CDS             27104..27487
                     /locus_tag="KRH39_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HBG8510382.1"
                     /translation="MAFKGQPTPSTITQITRAKISDGKSVRVILSDGESTKTQQFYLI
                     NGFFGVAMQDGEKGDEVTLQIEQAEYETDNIVTSEAFEAGELIYWDNTAKKFTTTSTS
                     NRLVGRVTDGKDSNNVIWFILLPQQ"
     gene            27506..28543
                     /locus_tag="KRH39_000455"
     CDS             27506..28543
                     /locus_tag="KRH39_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBG8510383.1"
                     /translation="MAFKVISQENLLEQKRKETLQEDIPFIVNGEMEYVTKKISNGEM
                     ETLELNKPLGEMMTFSSTSNLKELLRKVVLDVELGREQVQLLYKPIYDSIADSNLPQV
                     MDAKWALQGNCVFLEHIEGEEIKFGTINAENGPVARIQTYATGFEYTKEMKDFNQTFS
                     VEILNKSIGESYNALLNHIHLSPIINFNYKASNKTAFKGETNDPIWLGIWRTLTQAQK
                     DTVIAKRQGNILMASSADQIEIEMALNGGHLLNGSMYPSIKNISTVIYYDGWEVTVGK
                     KTYSYKGVTPGKGYLVRPKRGFKELIKRDLTTEVGNADLSKLVENQIVGHCYRGAFAA
                     VEENVQEISFR"
     gene            28583..28921
                     /locus_tag="KRH39_000456"
     CDS             28583..28921
                     /locus_tag="KRH39_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510384.1"
                     /translation="MTPTSNLIEKLRLLLNDKDKKSFTDEELNLFLEEADCIYCAASQ
                     GWVLKSLQYENTVGEMYEYKVGQETYKSSSIKDLVSVAYQNADKFKDMCTNKKEKGSF
                     MLGISTEFEI"
     gene            28918..29295
                     /locus_tag="KRH39_000457"
     CDS             28918..29295
                     /locus_tag="KRH39_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510385.1"
                     /translation="MINIDRRRKDIIRTINMNPTNITITSIKKTEIDGAFEEIETEIK
                     CTVRIFNEKTAEKQISSEKQGTFSSIRTYGMLVSSDVVLEINSRDFLEFECIYGKMKI
                     VNIYPQIVKGELCGYQCSLERID"
     gene            29297..29707
                     /locus_tag="KRH39_000458"
     CDS             29297..29707
                     /locus_tag="KRH39_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510386.1"
                     /translation="MSAFTNAINGINRKKAGMFVLCMSASAMLEGEAKANASWTDRTS
                     HARQSLNAKTLGGGNNFIIRLSHGAEYGGILEEGSKPHVITPKSAQALYWRGASHPAK
                     SVQHPGTKAMPIIKPTIDKNISKIGNIIFRYWSD"
     gene            29710..29886
                     /locus_tag="KRH39_000459"
     CDS             29710..29886
                     /locus_tag="KRH39_000459"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510387.1"
                     /translation="MKYTLANIRPLNNFLDFNLESFEILTKKMPIDILKPKVELISLD
                     YGEVKLIKKYTDCI"
     gene            29904..30725
                     /locus_tag="KRH39_000460"
     CDS             29904..30725
                     /locus_tag="KRH39_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510388.1"
                     /translation="MRAGIRKALIDNIKELKGCYEPNVPNKDTKKPYMVVVQGQDNDN
                     GETIGFERSIEIWIYEGRTTFKKLDKLTKQVVEVLDMNTIVDESENEAFTCIYKGTSE
                     NDIVVEEWDAIARGIRFSVIALEDKEDTTNDRWVEALSKYTKDSLGVESYKDNWKKNF
                     IAPCCLWRATNIENKRINYHLIEITKTMKCHVVSKNKDEIVKLLEALETSLIIDKRVR
                     LREDKNMYLTLVSVVEDRESDMFTTGQLTAVFKMIGKIKREGPTMDKIYGNGNLK"
     gene            30738..30941
                     /locus_tag="KRH39_000461"
     CDS             30738..30941
                     /locus_tag="KRH39_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510389.1"
                     /translation="MAETNNKKINVSKQEEKYLKSDFIKNSEALGYKKEVVAGALFNC
                     KKEELTKSEFEKAIKEFLEREVK"
     gene            30945..32366
                     /locus_tag="KRH39_000462"
     CDS             30945..32366
                     /locus_tag="KRH39_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG8510390.1"
                     /translation="MATGTWNEKEKKEIPGFYNRFKTQAEKSTNTGLKGRLAMPIKAN
                     WGEVGKVVTIKNDLRQLKTLFGDDMSYSAYKLGKLALLGNVKELLLYRLVDGNQKKGT
                     LTLKDTTENSAKDVIKLETKYPTSRNFNVTIKSNLVDADKKDFIFFEGTKQLFSSSVK
                     GTIDEIVLEINSNLDNEYVIAAKIADSDTTLANLVNVALEGGNDGCTSITNESYLKAL
                     EEFERYSFDSFVLDGVAEEALQETTKAWVAKNKELGKDILLFLGGKTEDNIKQINDKS
                     KGFNDENIVNIGSSAYYENIKYTPSEVAVYIAALSVSKGITGSICNAKTIFEEVEPRL
                     SQSEVKECLKSGTLILDFDDGDVIIVDDVNTFKKYVDDKNEAMGYISNIMFINTINKD
                     TSLKRKEFVGKIFNDSTGQTTVICALKKYFEELMSQGIISEFNVDIDTELQATAKADE
                     FYWKWDAVKVDVMKKIYGTGYLG"
     gene            32382..32795
                     /locus_tag="KRH39_000463"
     CDS             32382..32795
                     /locus_tag="KRH39_000463"
                     /inference="COORDINATES: protein motif:HMM:NF020949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510391.1"
                     /translation="MYNDDYIEEASFLNGSDVVILIDGVEELYMEEIKADFEQDEQSI
                     KLLGCQNEISRVGTTKGSFSLNGYKTDSKFAKLGFRSFEIIYNLSNSETLGYESIRLK
                     NCRLKKLPLINSKAGEIVKIEVEGSFRGYDLLNEL"
     gene            32879..33376
                     /locus_tag="KRH39_000464"
     CDS             32879..33376
                     /locus_tag="KRH39_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510392.1"
                     /translation="MSEMYKRELENEAIEREDNDLDEEIEETNEDRLKMKEDEIIAKL
                     LEDSPVPERTVFLDRLGIPITLKALTEKEISRIRKDCTKIVKVQGRREEKLNDDEFTL
                     ALIEKGTVKPNFSNQKLLDAMKVTNAREFIKRKFLAGELSKISDQILELSGFYDEISD
                     DDIKN"
     gene            33553..37827
                     /locus_tag="KRH39_000465"
     CDS             33553..37827
                     /locus_tag="KRH39_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBG8510393.1"
                     /translation="MREIAKKEMYHIDVVISAKGDGETKSKLSAMEKYMKQTEKRMQT
                     LNRIKVNPAIKATDKASSVVNRVNNNMNKAKKTVTARIKATDNASPVANRASNNVNKA
                     KKTVTARLKATDNASSTVNKVNNKIKEVAKPVPPVIIRGQDESSSIIDKVKAKIQNLK
                     ADTIIKIKSQADEAINTISRTKNKLQEFVSKRYEAAVKIRDEASSALRGLTGKIDSFV
                     SGAISKFARLATTAGALIGGIGVGSAVKGFATFEQSMKNTQAVSGATGKEMEALTAKA
                     RQLGRETSFTAKDAGDAMYYMGMAGWKSEQMIKAIPDVLNLAAAGGTDLALTSDIVTD
                     GLTALGMTANDTTEFVDVMAATITNSNTSVELMGETFKYVGSMGGALGVSMKDLSLAT
                     GLMASASVKGSMAGTSLRGGLVRLIKPPAEAASAIKKYGIELKKNKDGSLDLAGTIGS
                     LREKLGGLEKVEKGAAIQSIFGRTAMAGWAAVVNASESDFNKLTTAIAESEGEAKRIA
                     DMKLDTLSGQFEILKSAIDDVRISVGSKLGPMTRQFVEDLIKKMPQIGDAIVGVVEKF
                     VNNFDKIKAGFQVLLPAIGSVIGAIIVLKGVFAFGSAIKSLSLLANVFGTAKLAALGL
                     TAIIGAVVIAFAGMTVAISNNKIAIMDLQTRFGSFGEYITTIMESIGGVIKLTLGNLL
                     IMLGGIGKGIGILLSDKSWDEKASSLKNLFGKTTAEIKTNTKEALSDINSETSNATAL
                     LKKSTSKELQGVTKAFSTAFDQSKNVTEKKSSDIARALTNGLKGLDDQSLTMMRGLND
                     NMAIILSGVTADMKPSDKVNKITKNLDDAFKAGKLSAQEYRSSIQETLSFISKYSADS
                     SNNLKQGMSDAFNAFKEGTNVGGLKDGVTGMLNSLKATGPQALETLKSLGGKASEIFK
                     GVDFNSSIDAQKTKVLQNLNNLGLEGTQAIDTLRTIFSQASTALDTTNLKQGLSDTFN
                     SFKIGFNQDGINGAINSMLGTINQIGPQMQEALGKMDGKMGQVFANVDFSTPIETQAS
                     KVLENLKNLGIEGPQALETVRSIFAQASSQIQGSASQTAQQANQQVANALTQENPAVQ
                     QAGQQLGTDLTNGVVNGIQAGVPAVQQKGNELGVATQQGVTDALNNATPQVNNSNLTT
                     GIDTAFNQATATVQQGATNMYNGAKQSFSQLAQVGKEAGSSLYNGATTSFNMLASNVR
                     VACSSMYNGARTSFTSLSSSAISVISAMCSSVVSQVSAMSSQVISYWNSVRATVSAPI
                     SASFNVKTTQTTVKRTVNESSGGILSNIFGRFAEGGVASKPSICGEDGAEMVIPLSNA
                     RRNRAIGLYEQTGKMLGLGSTPSQKVVGSSNVVNNVRQFTNILDSDNKEYKEAQTQSL
                     VSSNGNTINLGGISINVQNSDNKEEMIQEIMSQVENELREALQDIG"
     gene            37909..38637
                     /locus_tag="KRH39_000466"
     CDS             37909..38637
                     /locus_tag="KRH39_000466"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510394.1"
                     /translation="MWQKFKSLNTFLKVIIGIIAICFLPITLAVFSIEFLMKAIEAKN
                     KSKIILGCFLALLTLSCARTVYLTRDLDDKNSTQVNQVSSNKDKEDAEKKIKEETKKQ
                     EEVKKKEEIEKNSKEDNKGKVETKKQTLTGEELKKKVDSIIPAEYKGNYYTNDVLDTD
                     GSYVLSLQVQNASFDNESSCKVFTKDLINKLKEFRIDSAEIYFVGSSGQTTYQINIDD
                     FLKVQDNVNSVDNMEFFSFKDFKN"
     gene            38707..39378
                     /locus_tag="KRH39_000467"
     CDS             38707..39378
                     /locus_tag="KRH39_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG8510395.1"
                     /translation="MVIDIYLKNEKEKIDFHFPVTPQDSLSIKKEKRFETVDIVNLGE
                     FDIKKEGEKIREISFKTFLPFQYDVSYCRYSELKNPTETVAMLEKWVDQAEPLRLIIT
                     GFGYNGLVTISSFSNTQTAGREEDRDIEITFRTYRELKIETLKKETKSNAKTDLKDNR
                     PNTQSSSKTYTVTSADALWSIAKKFLGKGSRWTEIYNIPENKKVIGKNPNVIKKGQKL
                     VIPSK"
     gene            39375..40337
                     /locus_tag="KRH39_000468"
     CDS             39375..40337
                     /locus_tag="KRH39_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ"
                     /protein_id="HBG8510396.1"
                     /translation="MKIILNGKYDIANFNEGITLSEAIDGVAYKMDISLVEPKQLQDI
                     GIKKGDKIVLIDIAYESKKEETIFNGVIWETRRSEKSKKLTLSCRERTVYMEESEEQY
                     SFKENTATQRIEYYCKQWNIPYYNLANTGKKLAKVIHKTNILDMIKKDLKETASKGGD
                     LFRVRMDNKLKLFKLGTNVNVYKLDSILEDANFTSSFNDAVTSVKVLGKSKDENTKAP
                     IIGTYKKDADKFGTLQKIKQDEKIKNAKEAKKAAEAMFNSGEETISVDCAVDINRIRA
                     GDKVSLKGKEYYVIDVTHTLDSKPKMKLNIGTLEYIRRKFYTND"
     gene            40330..40695
                     /locus_tag="KRH39_000469"
     CDS             40330..40695
                     /locus_tag="KRH39_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510397.1"
                     /translation="MTDARFNGVARILKEKMNKSVNDGVFGIGCELAEITANGLKVNG
                     YKDEIQDYLVLENLTLKEDYFTLSDEMLGAEYRHKHKIETPKELKPLAIGDKVLVAVM
                     GAEFVVIGRVVNAKPISSK"
     gene            40670..41131
                     /locus_tag="KRH39_000470"
     CDS             40670..41131
                     /locus_tag="KRH39_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG8510398.1"
                     /translation="MPNLFPQSETFETVELKNNNENELDLKGSFLFDFIKGEFVKNAD
                     GTLKKCDKVQAYKQWCQKAILTPRYKRSAYSSVYGSEIKELIASNLSKNAKELEISRL
                     IKETILVHPYTKEVGEFNFNWLENSRLVEYEFDVLTIDDENITIDGNIKGR"
     gene            41136..42272
                     /locus_tag="KRH39_000471"
     CDS             41136..42272
                     /locus_tag="KRH39_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG8510399.1"
                     /translation="MERELPIPIFLTEDEDSVHERMLSNFQDVSTLEGDFIYDATRPT
                     AEQIAELKQLGLQNNLKIAFPQTSYGTYLEWLGECKGVFKNQPTKSVGMITFTGVQGT
                     IIKKGTILTTVATDEKQSIEFELLETKTIGENETVDIKAECRVAGITGNVSKGSVSVL
                     LGSINGIKSVTNKEDFKGGTDIEDEEHFRERVLVAEQEDKLSGASSDYIRWAKEVNGV
                     GYAYVVSEWAGPGTVKVLILDKNRKAATQELIDKVQEYIYPLNTEEGQNRDGKAPIGA
                     IVTIATPQTLLINVKASFVFSNGFSQETVLNILKNKIDKYLDKIDIGGTVSYNAIYSL
                     TGSMMLADEGIQDFSNLTINDGTTNIILNDQVVGIGEIINEVIA"
     gene            42269..42916
                     /locus_tag="KRH39_000472"
     CDS             42269..42916
                     /locus_tag="KRH39_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBG8510400.1"
                     /translation="MITSKKGREMLLTLSPIYEQSLVMNSIYEAIGSEFDNLGLLNKE
                     IELQLFPQTATWGLEFWENRVGLITNLDEDMETRRRKVIAKLQSKYIMTPKRMSMILQ
                     SYTGADIKINENISPYTFSVELTSTQGFPRDLEDLYKRVNVIKPSHLAVSYKLVSLLK
                     SKTYFAQTAIMSEEITVYPYTSKEVKASVKAKFALAHNMSSETLTVYPKGGGTNG"
     gene            42909..43754
                     /locus_tag="KRH39_000473"
     CDS             42909..43754
                     /locus_tag="KRH39_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG8510401.1"
                     /translation="MADEQFYTILTNIGKAKIANAGMLGKSVVLDKIQAGDGGGNYYN
                     PTEDQTALKNKVWEGNINAFDKDENNPNWIIATACVPGSIGGFTVREMALIDNEGDMI
                     AICKSPETYKPKVGNGAMKDLYLKFIIEVSNVEKVTLVVDPTAIFLTKKDEEKIVASI
                     SKLDTKIDTTKTELKSNIETAKTEIDEKIGDTTQLTTTDKTNIVSALNEVKTSVDSIE
                     TTAEKTSIKDTDNLFESDNVEGALKELAENYNTLSEKQNNLETEVNGQRTKGITIVNN
                     LIDMI"
     gene            43768..44406
                     /locus_tag="KRH39_000474"
     CDS             43768..44406
                     /locus_tag="KRH39_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510402.1"
                     /translation="MTEKLTENASLGELMAALENVQTDFQTGKNNISSALGSPFIGTD
                     KFGTTKTKIETLKNVLVETINSKNVSATSSETFTNLIEKVNWIFQSIEIFSLKNRIQA
                     TTLNTPSIVYNEVSSIKGTLRFTGELKASKMRADYATAKIEILCGNRKEYFYVTDDTP
                     SASSSFVRFTKDIIVENSMDIKVQILLTSVGAGNLDGSYAARAEIEKLTILR"
     gene            44416..44628
                     /locus_tag="KRH39_000475"
     CDS             44416..44628
                     /locus_tag="KRH39_000475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510403.1"
                     /translation="MKKQVYYNSLDEKEKIISENSNLYVIEIYETLNENYLVLSSSPI
                     EDEKLSYEELENELLIMTNELQGGLL"
     gene            44628..44810
                     /locus_tag="KRH39_000476"
     CDS             44628..44810
                     /locus_tag="KRH39_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510404.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNRVFELDDVTNTEYRMAVEN
                     YIIEHSGVVEGTEPAK"
     gene            44845..45075
                     /locus_tag="KRH39_000477"
     CDS             44845..45075
                     /locus_tag="KRH39_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBG8510405.1"
                     /translation="MNEEVIKEKIKRNEIRINRHSDEIDELKVANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAVQRGIF"
     gene            45094..45354
                     /locus_tag="KRH39_000478"
     CDS             45094..45354
                     /locus_tag="KRH39_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG8510406.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPIILLVL
                     GIGFSIWMLGFSPNAVLQGVICWGISIGINQTYKQLKEENKQ"
     gene            45351..46148
                     /locus_tag="KRH39_000479"
     CDS             45351..46148
                     /locus_tag="KRH39_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8510407.1"
                     /translation="MKIAIVPGHTLTGKGTGATGYIDEGKENRILTDLIVKWLKQGGA
                     TVYTGKVDKSNNYLAEQCEIANRQNVDVAVQIHFNADHTTLNSMGTETIYKTNNGKIY
                     ADRVNTKLATVFKNRGAKSDVRGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKNINNEGVKQMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIKDYV
                     PGQTQNLYVIGGAACEKIGSITKEKYTMINGSDRFDTLYKALDFIDR"
     gene            46561..47589
                     /locus_tag="KRH39_000480"
     CDS             46561..47589
                     /locus_tag="KRH39_000480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510408.1"
                     /translation="MNKELVYGEIDEKNLDVSRISHEELKALFNLMNGKPDSMLRIFP
                     RDVNVEIKDLYSLHEKIKEKLQHYIVAALIVNISVKFDNNTFKEFSDWIEFQNCNWSS
                     SHYLENITIKYDFFVDFKEYGVPQRHTLVIRLSSGMKPQQILQLIVSGQIEEIDSIDK
                     SLSPVTCKVDFTNHLLGDELINIIEEWDKSIQKVVSESGKTMKFIRKYRKGISAVIEN
                     IIPLFGLIVLVSIVNFNLSHFNIDTLVQLSISHIQKLIMLTLLFSLIYKVIKNIGEFL
                     SNKFLTEIYEYGDTHIFNITNGDKIKREKLDIKNKKHEKGIKSSLSLTILINVICGIV
                     ATYICTIF"
     gene            complement(48184..48372)
                     /locus_tag="KRH39_000481"
     CDS             complement(48184..48372)
                     /locus_tag="KRH39_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510409.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHGYSKK"
     gene            48628..48804
                     /locus_tag="KRH39_000482"
     CDS             48628..48804
                     /locus_tag="KRH39_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510410.1"
                     /translation="MKTLMFILVIAYYITIVIKSITTIVKSITRLLDSISRLKESLHK
                     LRNRPDGNQDDFNE"
BASE COUNT        20158 a         5247 c         9063 g        14572 t
ORIGIN      
        1 gattttttag attatatatt ttaatatact ttcttatata agaattattt taaatcaaga
       61 ttatctagta tttttaaaaa tatttttaca gcagtatcca agtcatctac agaaacactt
      121 tcattaggat tatgacttac gcccccagcg catcttataa ataccatacc aatatcagtt
      181 atattatcca tttcctgagc atcatggcca gcaccactat aaatataaaa aggattaaga
      241 tttaaatcta taaaactttt ttctatgatt tttgttattt tattagaaca tggtacagga
      301 acattttcaa aagcaagttc acttgtataa ttaagttttc tattttcaca tatatgtgat
      361 atttcattaa atatcttttc tacagaatta tcaagaattt cttgtgaaca agagcgtaca
      421 tcaagagtaa attcgattat tctatataaa atggctaaat agctggattt aagtaaaaaa
      481 aatagaattt atcattattt tgccacccat ttgccaccgt aatttttttc ggtggcaaat
      541 tatctaacaa gattttctaa aatatctatt gtttcacttt ccattttact tgtaacatgt
      601 gaatatgtgt ccatagtagt tgataattga ctatgtccta atctttgctg tatatatttt
      661 atattagctc cattttctaa taataatgta gcatgtgtat gtcttaaaca atggaagttg
      721 aagtcaaggt ttaatttttt gttaattgtt ctcacacaag catcaatgtt gttatgattt
      781 acaaatgagc catctttttt tctacaaacc caatcatgct cagtttctgt ataccatttt
      841 ccaagtttaa ttttctgctt tttttggttt aatttttctt cttttaatat tttagataaa
      901 gtatcaccta ttctaatatc tcttatggat gtttttgttt taggtgaagt taattcaaac
      961 tctgaaacct ttctctttat taaatttttt ctaactttga ttatattatt atctaaatca
     1021 acattatccc aacaaagacc taatatttct ccttttctca ttcctgtatg aaatcctata
     1081 agtagaggaa tatagatatt tgtattttta ggatatatct ctagaatttt attaaattca
     1141 tctaatgtta tagttttgtt ttctgaatct ttttttgtat ctataatatt ttttggtatg
     1201 ttggtgtatt gagcaggatt ttctttaata agcttgtaag gataaacagc agattttaaa
     1261 gcagagttta atacaccata gattgctttc aaattacctc ttgtatagcg tctttcttct
     1321 ccgttttgag tatatgtttt tgttttattg ttcaggaact cttgaataat agctggattt
     1381 atagatttta atttgtattt gccaagttca ggttctatat ggtttttaat taaatttcta
     1441 taatttactt gagtattata tttacaatta agaagtacat attctttata ccaaaagtct
     1501 aaataattag ataaattcat atttgtttca tcaaacacga taccagcatt ttcgtattca
     1561 tttattgcat ctcttaaagc tttttcagct tcctttttag tttctccacc aactttttct
     1621 actttttttc tcttaccatc tacaatacct gcatcaaaat agtaatacca cttcttgcct
     1681 ctttttctta ctccgccttt cataaaactt ctcctctcaa aattaaataa tcagagctaa
     1741 atgctctaac tatattataa caattaaaga tactttaaaa gatacatata ttaaaaatag
     1801 atatatggaa aataataaca taaattataa ttgatacaat tttaaaatta tagagaataa
     1861 gattataaaa aatgatttat tcaaatgtta cttaatgtcg ttaaatgagt agaaatagtt
     1921 tgtactttcg acaacatata atatattttt gttatacaat ttaattaagt gacatgaaaa
     1981 aaagggggag atattttgaa atcaaaaaaa ttattatcat tagggttagc tatgtcaatg
     2041 tttgttgctt gtcctatatc tgcaaatgca cttgataaaa ttgataaaat tcaaggagca
     2101 gataaatatg aaacggctgg acttatcgca gataagcaaa gttatactac tgcaatactt
     2161 ataaatgcag atagtactat ggctgatggg ctaagtgcaa gcggacttgc aggagctaca
     2221 aacgctccaa ttcttttaac taagaaaaat aatataccta atgcaactct taaaagagta
     2281 gaaaaagcta aaaaggtata cataataggt ggagaaagtt ctattgataa agcaacagaa
     2341 acttttctga aagataaagg aatagaaact aaaagacttc aaggtagtga tagaattaag
     2401 actagttata atgtagcaaa agaaataggg actattaata aggttagtaa agttatatta
     2461 actaatgctt ttaaaggcga acctgatgca atgagtgctg catctgtagc tgttagagat
     2521 aaagcaccta tagtattaac tgatgggaaa agtgtaccat ttaacactac tggaatagaa
     2581 agttatgcaa taggcgggac ttcatcaatg agcgagaaat tagttaatga tactaattca
     2641 actagattag gtggtacaga tagatacgat actaataaga agatagtaaa taagttctat
     2701 aatggtgcaa aagaatttta tatagctagt gggactgatt tagtttatgc tttagttgct
     2761 tcatcaatgg ctaaaaatac tccaatagtt ttagttgatt atggaagtga taaaggtatt
     2821 ttaactaatg caactaaaat aacatcagta gggaatttat ccgaagaaat taaacaacaa
     2881 tgtttaaatt caaattcaaa agtattaaga gatattttag gtgtttggaa tgatgaaagc
     2941 tataatgggt actcaatatt cggtataaca gataatacaa taggtgggaa tcgttatgac
     3001 atattatcta taaatgaaaa agagaaaagt attaaaataa atgtacatta tcaagatgga
     3061 gattggaaac atgttataaa atcagagaat agtaatgaaa tttggtttta tacttataat
     3121 gaagtaaatg ataatttttc tggtggtgta ccatatcaaa gattatatag ctttgatagc
     3181 tcagatacta tttataaaaa tagtgaatat ggatttagtt taagaatacc aaaaaaatgg
     3241 gatggtaaat ataaaattaa tgtatcaaaa ggctcaaatt ataacggttc agaggcaaat
     3301 atagattttg attatataga aaatggtaaa gtatatgata atctttttac tatatatgtt
     3361 tttaaaaaag gatatgtaca tgactcagaa gatgagttag tctatatggg aacaacaaaa
     3421 aataaaacac tttattttga gatagatggt gacccttctg tagaagcttt agaaaacgaa
     3481 aaagttttaa atcgacttat tcaaatgaac aatgaagcta tgcgtgtgtg caatagtttg
     3541 aatttattat attaatagtg ttaaaattta ataattataa aaactaataa agcaataata
     3601 agtataacta gatttaaata attatgcaga ttgtaaatta ttaacaacat agaaatagac
     3661 tagtgtttag ctagtctatt tttttctttt cttcttttta attcttccat tataaatata
     3721 gctgcgataa ctaaatagtt aacttctaaa ccaattgcta ggtcaactat tgtagtaaca
     3781 gttacatttt tataattacc tttttctaat ttagatatat aacttttgtt ataattaatt
     3841 ttctctgcta actctaattg tgttaaatgt ctcatttttc tcagttgtct taacataaat
     3901 ttaaatcacc ttttaatata ttttttagta aattcagata attatattat gtgcattttt
     3961 gtggggaaaa gtttcctatt agtcgatttt tttgttggaa atttgtgcta aaatataagt
     4021 aagaaataac tctatctagc tagagaaaat ttgaaaatta tatatttaaa taagctttgt
     4081 aattaagttt atataagtgt aagaacttta tttccaaaga tgggaggatg attataatta
     4141 atttgacgaa cgtaagttct tgattgttaa tcacataatt tttataacaa atacataata
     4201 taggggatga tacatttgaa taaagaagaa tattttgaaa aaaatagatt taaattagaa
     4261 aagattctta aagcagaacc caaaaagtat gaatatataa taaaattaat agaagaagaa
     4321 atgagtaaaa aaaataaaag agtgtaaaaa gctcttttat ttttcttcat ttattatttt
     4381 atagtaagcg ttatcaataa ttttctctat tgcttttctg ttctcctcat ctaaggaata
     4441 taatttgtgc aaagcttgtt ccatttcttt gtttaatcga ggatttaggt ctagattatt
     4501 ctttcctaaa agataatcaa ttgatacctc aaaaatttct gagaatctta ataaatcttc
     4561 tttaacaggg tctcttttat ctgtttctag cattgcaatt ttactcaaag aacaatttaa
     4621 taattttgcc aactccattt gtgttaaacc tttttgcttt cgcaactctc ttaatctatt
     4681 agaaaacata atatcactcc ttgtcatgtt tatactacga ttgtatcact aacagtgata
     4741 caaataaatc cttttttaaa aaacttcacg aaaagagata aaaagtcttg actatattca
     4801 cttaaagtga tattattaat ttatcacaaa tagtgaaggc gaggtgtaac atgaaagagt
     4861 tatcaaagtt tagaaaaaaa ttaggattaa cccagaagca attagctgaa agaataggca
     4921 tatctaagtc ttattattca aagatagaag gaaattttaa aaagccagga agaggatttt
     4981 tggagaaatt taaaaataca ttcccaaatg aagatatgaa catttttttt aaacaataat
     5041 atcacttaaa gtgatgtaag gaggattata aaatgaataa cttacaagta atagaaagaa
     5101 ataacgaaag agttctaact acacagcaat tagcagatgt ttatgaaacc gatgtaaaca
     5161 atatacaaac taactttaat agaaacaaag agagattcaa agagaatgta cactattttt
     5221 tattacaagg tgagtatttg aaagaattta agaaccaacc tactaatagt cagttggttt
     5281 caaaacattc tagtcaatta tatctttgga ctgaaagagg agcaaacaga cactgcaaaa
     5341 tattggatac tgacaaagct tgggaacagt ttgataactt agaagaaaca tatttcaaag
     5401 ttaagcaaca aaaaccatct tgtatagaag atgtattaat agaaagttta aaagaaatga
     5461 aagatttaag actacaagtt aaccaagcta ataacattgc attagaagca aaaacagagg
     5521 ttaaaacaat aaaagaagtc gtttcattaa atgctactga ctggagaaaa gatacacaac
     5581 aactaattgc aaaaatagca aaaaaacaag gtggatttga acatataaac ttgcttagga
     5641 gagaaagtta tgagttacta aacaataggt ttggagttga cttaaataga aggttaataa
     5701 ataaaagaag aaaaatggca gaagaaggtg tatctgaatc taaaagagag aaagttaaca
     5761 atttagatgt aatacaagat gataagaaac tcatagaggg gtatgtggct attgtaaaag
     5821 atatggcttt aaaatatgga atatcaagtg atttaagcaa aaattaagtt aaatcataac
     5881 agcactttga aaactaaata taaaatattc aaaagaggtg attagatgca aagagagcaa
     5941 acaacgcttc gtataccaga agacttgcac aaagcgttga tagatttaag cagtgatata
     6001 ggaatgccta ttacatctat tataataatt gcatgttggt tgtatatatc aaagataaat
     6061 taaccaatcc gatatgcaaa atgaatgcac agcattattt attagagaat tgattgataa
     6121 attattttct ttagctgttt tctctagcaa ctcttttagg acaccaggaa tcctaagagt
     6181 agaacgaaca gtatcatcac ttttatacga gattgattga agttcattaa cttttgaatt
     6241 tctaataata tcattaatag cgtatagaat aagcgaagat ttgagaatgc ctgtttgata
     6301 agaaattttt tctaaatttt catttaatgg tgtatatatt cttacagtaa tagtagtcat
     6361 tttgacacct ccctaatgac attatacaaa aaatatttaa aaaagtcttg acactaaaat
     6421 agtgtcataa tataatgatt atagacagta aaacagtgtc ataaggaggg aggaataaga
     6481 atggaaaaag taagaatgac agtaaggctc gtcccaaaat taaatcaata tgtacagaat
     6541 atagccaaag aatgtggaaa aagtaaaaat tctattattg tggatgcatg ttgggagttt
     6601 attgagaaaa taaaaaaaga aaataaattc aatgtagaaa gcgaggaata aatatgaata
     6661 atctacagat atttgaaaaa ttggaatttg gacaaataag aatggcagag attgataata
     6721 agccttactt tgtaggaaaa gatgttgcaa agtcattagg ttataaaaat actaatgatg
     6781 caattttaag acattgtaaa ggggtagtga aacacgaggg gtttaaaatt aatggtatta
     6841 agattgcttt gataactgag ggtgatgttt acagattaat agtaggctca aatcttccaa
     6901 acgcagaaaa gtttgagagt tgggtatttg atgaagtgct tccaactatt cgacaaactg
     6961 gtcaatatca agcacaacag catgtaataa cagaacttac aggaactata ggagatttaa
     7021 aaggaacaat aaaagaatat aaaaagttgt gtaagataac atgctctaag aaacaacagt
     7081 attctaaata cataaagaat agacttgata tagacaaagc taataaagaa tacaaccaag
     7141 tgaaagcaag gctattccta attcttggag tggagaaatg ggaagatata gactttgaca
     7201 catctaataa tttaatacag attatagatg attgtataaa ggttataaag tcagaaagac
     7261 catataagca gttgagttat tttgaaaact aaatacagaa tattttgaaa tatattgttt
     7321 taattaatta ttaactagga ggttaaatgc atgaaaaata atacaagcga ttcaagagta
     7381 aaatactttt gcaaatgtcc atactgcggg tttgataatg aggtagaagt taaaaaaggg
     7441 ttgaagccta agacatgttg catatgtaca aaagaagttg agtatgaaaa actggagcaa
     7501 caaagtgctt cggaaaatac agaaattaaa ggagtgtgta attaaatgaa aatttcattt
     7561 gaaagtgtag tgaacaatgt aaataatgaa acggaattaa tattatcaaa agaagagttg
     7621 caaatagcta aaaggatact taatacattg aatgaaaatg aacgaagcat cctttcctca
     7681 aaagatattt tagatttttg taaagaagct cttaaatata atttagtacc taaatttatt
     7741 taaaaacacc gagaccatca cctttagaaa aactattatt tacttctgaa agtttcaaaa
     7801 agatttcatg gtaatgtttt aaaagttctt cttcagaaga atttttaaaa tcatattttt
     7861 ccttaagaat ttctaaagta agagtatgta atatatcttt actgaactgc ataatatcac
     7921 caactttcat aaaaagatat aggatatatc ctacaaatac attatatcaa aggagaaaat
     7981 taatggcaat taatgacaac ataaacaaaa ttttaaaaga taaaaattta aaagcatgga
     8041 aattagcaaa agaaataggc gtagattcag ggaatttata tgcaatttta agaggagaaa
     8101 ataaaaatcc aactatagat acattaataa aaatagctga ctatttagac gttacattag
     8161 atgaactagt tggaagataa aaattaaata cagaatattt tgaaaaggag tgagtaaatt
     8221 gggcaatata tctaatttca atttagataa acaagaagat aaaagtttta atgacctaga
     8281 taatatatca atttgttttt cagaaggtat ccgtaaagtt gtagaaatga atttaaacaa
     8341 ctataaaaat aaaatctcaa agtacttaaa tgaaacttcg aaaatagaat tattagaacc
     8401 aaaagaacta acaattgtta taagtaaagg ttatcccgat tatcttatgt ctgttgaaga
     8461 agcaagtaaa agattgaaaa tagataaagt atttggatat gagttaataa aaaatgggct
     8521 tttgaagtca gttgatatag gggcaactaa agtttctagt tatgaattag atgattttat
     8581 aactagaaat caaggaaaaa acatcaaaga aatgcttagg gaaatgaaag aaattagaga
     8641 gggggtgatt tagttggaat tggtaacata cagaaacaag cttgttttac tcaaaaatga
     8701 aaaaaagatt gcaactataa gtttaaaaaa gaaatttctc agcaacagac ttaaattaaa
     8761 aataagatag gagagataaa ttgaaaataa tctataaaaa caaagtctac aaattagaac
     8821 aagacaaagt gttatttaga attacatact atgatgagca gagaggtagt aagaagttta
     8881 ataaagataa gaaagtaaaa agaagtgttt taacaagaga tatagaggta gttaacttgt
     8941 atttaccaac acatttaaaa ataaagtaag ctataaataa ttaaagaaaa aggtgattag
     9001 atgcaaagag agcagacaac aatacgcctg cctaaagaac ttaaagaaaa attagaaaag
     9061 caggcgagta aaaaaggaag aagttttaat agtattttat taagcatatt acaagaattt
     9121 attcagaatc caaatgtata gtaccatgtt ctttttcaaa catttctata tgtttagtaa
     9181 caagaaattc tatttctttg ttagctgaac gagcattgta ctcagctata tatttaagtt
     9241 tatcaagttt ttcttttggt attctaattg caaaatgagg atttttatat gtacccattt
     9301 tagccataat atcaactcct ttgtgtagtc tatttggcaa caattatatc acaaaaataa
     9361 atacaaaaaa atatataggc aacatataga caacataata ataaagtgtt atactttgta
     9421 ttaaagatag gcaacatata gataacaata caggaggaaa atatgttggc aaatagagta
     9481 agaactggat taagaatccc atatgattta aatacaaaat taatattaat agcagaagaa
     9541 agaggaatgt caaaaaattc tttaatatta cacatacttt gggattacat aaaagaaata
     9601 gaaaataagg aggtcgaata atggttgaat caacaaaaga atttgattta caaacaatta
     9661 aagcaggaaa tgcagtaaaa gtaaattgca aaagatttgg ttttgaaatt gattgtatag
     9721 tagtagtagc aacagaaaaa gaattaaatt tagcatactt tgatgaaggt agaggctgta
     9781 tggagtatca agccttaata acagaagata ttcaagatgg tgattatgag attaaaattt
     9841 tatcttagga ggaaataaaa tggtaggttt atttgcaata tgtttagcag gattatttca
     9901 ataaaaaaag tgctggtcaa agtaaccaac acatacaaaa aattcaactt atttaggagg
     9961 ataccataaa atgaataaaa tttcaagtcg tagaaaatat ttagatgctt ttatagtaac
    10021 tgatactaag aacatagata aaattgattg gcttaaaaat agacaattag gaataggggg
    10081 tagtgatgcg tcagcagtag caggattaaa tccctggaaa acttctgttc aagtatatat
    10141 agaaaagaaa gaagaaatac caatagaaac taaaagtttc agaatggaat taggcaatag
    10201 attagaagga ttagttgcag aactttttac agaagaaact ggtcttaagg tccgtaatgt
    10261 aaatggaatg ttgaaaaatg aaaagtatcc ttttgcaata gctaatatag acagagctat
    10321 agtaggagaa aaagcatttt tagaatgtaa aacaacaaat agtttttcta taaaagaatg
    10381 ggaaaatgga gttccacttc attatgaaat acaatgctta cactatatgg ctgtcacagg
    10441 agctacacat tgttatatag cagcacttct tggaaatgaa aagtttgtat ggcacaagat
    10501 aaatagggat aatgaagtaa ttaaaaatct aatgaaaata gagagtgaat tttgggaaga
    10561 aaatgtatta aaagacattt taccaattcc tgatggttca gatgcttata gtgagtttct
    10621 gaaaacaagg tataaaaact cagtaaaaga gaaaatagaa ctaaatctac ttgaagatgg
    10681 tatatcaaag ttaaaaagat atgatgatat agttttacaa atgaaagaac taaaaggaga
    10741 gaaacagcta atagaacaag aaatacaaag tgaaatgaga gagtttgagt tagctacatt
    10801 aggcggaaga ataataactt ggaaaggagc tactaaaagg tccattgata ccaagagatt
    10861 aagagaagaa atgcctgata tagcagaaaa atatacaaat ataagttcat acagaacatt
    10921 caaaataaaa tagggggtaa tatatatggc tagtgaaaaa gcaaaaggag cattagaaaa
    10981 gaaagtttca ggagcaaata cagtcaaggt aagtccaagt aaaggtatgg agcaacttat
    11041 gaataaaatg gcaagtcaga taaaaaaagc tttacctagt atggtttcaa gcgagagatt
    11101 tcaaagagtt gccctaacag cttttagtaa taatccaagg ttacaatcat gtgaacctat
    11161 gagttttata gcagcaatga tggaatcagc tcaattaggt cttgagccta atacgccttt
    11221 aggtcaagca tatttgatac catatggaaa taaagtgcaa ttccaaattg ggtataaagg
    11281 tcttttagaa ttagcacaaa gaagtggaaa gataaaaact atatatgctc ataaaataag
    11341 agaaaacgat aaatttgaga ttaaatatgg gcttcatcaa gacttagttc atgaacctaa
    11401 attaaatggt gatagagggg aaataattgg atattatgca gtatatcatt tggatacagg
    11461 aggacatagt ttttctttta tgactaaaga ggaaattata gaatttgcaa agagtaaaag
    11521 taaaagttat agtagtggac catggcaaac agattttgat tcaatggcta aaaagacagt
    11581 tataaaacag ttattaaaat atgcaccact tagtatagaa ttacaaaaag ctatggtagg
    11641 tgatgaaaca ataaaatctg aaatagatga agatatgagc atggtcgtag atgaaagcga
    11701 aagtttagaa gttgatttcg aagtaaaaga aaatatggat ggtaaagtta gtgtggaaga
    11761 atctataaat gttgattaag taggtgagac accttgaaag aagataagtc agttttagag
    11821 aaattaaata tattaagtgg tggatatggt cttatgccaa gaataatagc aagagatagg
    11881 tggttgacag ttggtgctag aatgctgtat tcatatctaa ctagttttgc agggaatgat
    11941 ggaacatgtt ttccatctag agatttaatt tgttatgaac tagatatatc aaaagacaca
    12001 tttacaaagt acaaaaaaga gctagaaatg agtggttata taaggattca taagaataaa
    12061 tccaaacaag gtaagatgca aaacaatata tatgaaatag tatttgatag aacctatata
    12121 gaagaatgta tttctaagag aggtttaaaa gtagataaaa agaagaaaaa accatgtcct
    12181 aaaaatatag acatggaacc atgtcctact tttccggaca cgactcagcc ggacacggaa
    12241 aatatggaca ctaatagtaa cagtattaat agtaacagta ttaataatat gtatatagga
    12301 aagaaacctg tggataactt tttaaaagaa tttaagaagc tgtatgaaga aaatatagga
    12361 gtaatatatc cagttacagc tgaatggtta ttagaagtat ctaatgaagt agatataaga
    12421 gtatttaaaa gagctataga gatatgtgct gaaaagatga atatgaatct agcgtactta
    12481 aaaggcatcc taaaaaaatg gaaggatgca aatataacta catatgaaca actggagtca
    12541 tatagattac agcaagaaag caagaaatca aaaaaagtag ttaataacca gctaagtaaa
    12601 aataagtttg ccaactttga acaaacattt actaagtata cagaggatga gcttgataac
    12661 ataattaaga aaagtcaaga tgctaagttc aaataaaatt aaacttctag gaaataaata
    12721 tcaatatatt acttcctaaa agttaaaaat ggagggataa atatggagtt aaaatttaga
    12781 gaatggaata aaaatggtaa ggaaatgtat agttatgatg aaatggtgtg ctattctaaa
    12841 aatttgctta gagagtgggt ttacagtggt gtttatttac caacaagcaa tgaaaatttt
    12901 gaggttatga tatacacagg tttgaaggat tgtgatggaa aagaaatcta tgaaggcgat
    12961 attgttttat gtagaagtat attttttaca gagtttgcag gggaggtaaa atttaaagat
    13021 ggttgcttta ttgcagtgaa tgaaatgtca ggagattgct ttagattatc tgaacttgca
    13081 atagttaaag ttgatgggaa tatatatgaa gatttaagta agttaaatga tataagaaga
    13141 tgtgataatg aggggtgtta ggaatgataa taattagaag tcaagataga ttatatttaa
    13201 tgagagttaa tagagttgaa ataagtaata gtgaagtata tgcaatgctt gaaaatgatg
    13261 ttagaaaaat aggtgaatat gagagcaata aaagagctat gcaagtgtta aatgaaatac
    13321 agaagtttat tgaaaatgga gtaagaacag attatataga ttcttataga attagacata
    13381 atcaagagga agtgtttgaa atgccatttg aataaaaaaa aggggggcta aatatggcta
    13441 aagtttgggt ggatgcagga acgtttttag aacgaactga ggatatagaa gatatgtttg
    13501 aacttaattt aagaaaagta agagatagaa ataagaaagt aaatgtaatt gatttgcttg
    13561 taaatgatga gtgtcaagga agaaaaggta aaaaagtaga gtgtttcaat attgtaactg
    13621 gagaaactaa agtatttgat agtgttgttg aggcaagtaa atatgtatat tttacagatg
    13681 tctatattgc tcatttagca agaacgggta aggtttctaa gaacggatgg aaagctagat
    13741 atattaaaga ggtgtcaaat ggtattagca aatgtggagc aagtaattaa gttagctgaa
    13801 aagatattaa gtaagaaaaa gtgttctgtt aataaagcta ttgatatagc tataaaaata
    13861 ttgagtaaat atgagtgcga ggggatattg aagattaaat taggaaaata attttaatta
    13921 aggaggcttg attatgttgt atttagcaag aatacagttt aaaagagaag aaaattcaaa
    13981 agttgaaagt ggatatatga ttggggatgg ttttactaat tcatgcttct tagatgaaga
    14041 ttttaatcca ttatcaaaag ataaagatgg tttttcactt tatgactata gattagactt
    14101 taagaatcca ttagaactta aattataaaa attcttagaa aataaggaga aaataggaaa
    14161 tgaaattaaa agatattata aaacttggag aaaagtattg ttattgtcct cattgtggta
    14221 atgacaaaat tggaaacaat gaaggaaaat tgatagttga agaacacaca tactataggg
    14281 aatgttcatg tggattcaat atattaattg atgatagaaa ggatgagata taatgcatat
    14341 ttcaacgact attttactgc taataggaag ctttatagct ggtagggttt atgagaacca
    14401 tttgagaact gaaaactgtg aagaatgtga aagtattaga gaatttatac aatctaatga
    14461 gctgtgtgag tattgttcaa aagaaattga tacatgtgat aaaaaatgtt acaaaaacat
    14521 tttgaagaga agaagaaagt attatataga tggagaggat gttgaaaaat gaatatctta
    14581 ggagggttaa tgttgttagg aataggcatt gtagttggta gggtttatga gtatagattg
    14641 aatctaaaag agtgtgaaaa ttgtgataat attggagggt ttaagaatga ctaactttga
    14701 aatgataaag agtttagaca aggatggaat ggctgatttt cttggatgtg ctgattgtat
    14761 ttgtgagtat tgtgtttatg aaattgaaac ttgtgtctat aaatgtttcg atggatacaa
    14821 aaagtggctt gatatggagg tagagctata aaaatttgga atgatgaaat aagcaaagaa
    14881 ataaaaagat taaaaaggtt tttatataca gaaaatatct aattaaaaca gtttagagag
    14941 ttacaaaata tcttttaata taaattattg tttgaacgtt ttgtaactct caaaaatgaa
    15001 aaataagggg tgggataagt gtatgaatat atacttagat ggcagatagg attgtcatta
    15061 gaaaatagaa aaatacatta cacatatgga agtaaagacg ttttaagaga gaaagcaaag
    15121 gtattggcta aagatgaaaa tatagtacta ataactatag ataaggtaga tgaagttata
    15181 aaaaacacga taagcgaaaa gattatagaa cgttttgaaa atttataagg ggtggaatta
    15241 tgataataca caaatttata atacatgttt tagataagaa tagtgataca ccaatattga
    15301 atgattttga aggtagggtt aatcaagata tggacctatt ttttcaaaag aaaataagca
    15361 aagtatcaag agataatgac atcagaacag cagtatttaa taactatagt aacaatctaa
    15421 ttaagaagtg ttgtgaacaa attatttatg atgaaagttc atttttaaat agctctaaag
    15481 agattgcagc ttatttattt gatgttatga aattgaatgc tacattagaa tcttgcgact
    15541 tagcaatttg cttatactct caaaaagatg aaaagaaagt tgctatatta aagcttgatt
    15601 acaataattc gtatactcat tcaattgagt ttaaagatga taaatttaat atacagatgt
    15661 ctaaaaatga aattaatata caagagacta agacagtcaa aattgctgcc ctagttggac
    15721 taagtggaat gaataacgaa tatcatctaa acgtattgga taaggatgca gagaaggaag
    15781 aagctaattc taagtttgtt acagagtttt taaacgctac taagataaaa gatgacaagt
    15841 acaagactaa gatgtttaaa agtacagccg aaaattggat aactaatgct cttggaaatg
    15901 atataaaaca ggcagaagat gtaagaagta tattaaatta tactttgaaa gaaaagcatg
    15961 aaattgatat aaatgatttt gttgataaat caattaaaga tgatgagtta aaaaatagtt
    16021 tcaaagaaca tatagaggaa aaaggtcttg ataaaagttt taatattgat aaaaaatggg
    16081 ttgagaaaaa gcttaaaacg agaaatataa aaactgacaa tggctttgat ataaaaggta
    16141 atctaactga ttttgaggac ccaatgaaat atacagtaag acaaaatcaa aatgggtcta
    16201 tagatataat tattaagaat gtaacatttt atgaagaaaa gtaggtgaac tacttgaaat
    16261 atatagcgag ttttagcgga ggaaaagatt cagcagcaat gctccttcta atattagaaa
    16321 aaaagcttcc attagatgag attgttttca tagacacagg gttagaattt aaagaaattt
    16381 atgacataat agatgatttt gaaaaaagaa taaactttaa aataacaaga attaaagcag
    16441 aaaaaacctt cgaggaatac ttttatactg ttaatcaaca aggtaagcgt aaagggcaaa
    16501 tatggggttt tccgtatact ctaggggcat ggtgtaatag cagattaaaa cttgctcctg
    16561 ctaataaata ttttaataaa ctaggagagc ataaaaggta tattggaatt gcctttgatg
    16621 aacctagcag atataaaaga ctagaaaaaa actgcatagc accattgttt gaagaaaaaa
    16681 tgactgaaaa agattgcttg aaatacttga aagaaaaagg tttctattat gaaatacatc
    16741 acagattcaa gagaactggt tgctatttgt gccctaaaca aagcttagag agcctaaaaa
    16801 cactaagaaa atactatccc attttgtggc aaaagatgct taagtttgat agagatagcc
    16861 ctgtaccttt tagagctaat ggggtcacag tgaatgacct agaaaagcga tttagtaatg
    16921 aagatataga aaatgaaaga cagataagtt tctttagtaa aggagtggtt tagaaatgga
    16981 tttaccagat aatgttatcg aaattgatat attaagaatt aatagaaata atgataagaa
    17041 atgtaagtgt aagaatagag aatttatagt agatacagtt aataaatcag ttcattgttc
    17101 agaatgtgga tttagagtag agccatatga tgcgctatat gaactagcaa tgaactttga
    17161 aagagtaaat gggcaagtaa acacattatt aaaacagaga aaagaaatag ctagttataa
    17221 acctcacttg atagttttta agaggcttga aagtaaatac aagggtaaaa aaatgttacc
    17281 agtttgccca atttgtggag aagcatttta ttttgaaaat ttaacttcat ggattaataa
    17341 agatttctat gaaagaagaa aaaaataaaa taaatagtca aggtaagttt gtgaatgaaa
    17401 ctagaaggct atagacttac tttgacttat aaaagaggtg agattatgat tactactcaa
    17461 atgtataaga taaataatga gatatttcta aataatatat gtttggaaga aaataaggaa
    17521 attaccatat gggtagaaaa gaataacaca catgaactta tatggttaaa aatagcgaat
    17581 gtaaatggca agttagctat atttgtacaa gatattgaag atgctatagt aaacgaatgg
    17641 caaggtcgta tagcatacga acgagtgata tcgcaaatag aagatgaaca agttgaaaaa
    17701 ggggaaatag actaaaactt ttaaagggtg tgagcttatg atacatgaat taaaaatatt
    17761 acctcaatac tttaaagaag ttgcaaatgg gaacaaaaat tttgaagtta ggaagaatga
    17821 tagaggtttt aaaaaaggcg acttgttggt attgcaggaa tttgatggag aaaaatacac
    17881 aggtcttgag acacgaaaag aaattactta tgtacttgaa aacagcgatt atctgcaaaa
    17941 cgggtatgtt gttttaggaa taaaataaat gttttgtgac taggaagtga gtttatgaaa
    18001 cgaagaagat gcagttggtg tggaaaatta ttttatattg atgaaaaatc taagggtatt
    18061 tattgttgca aggaatgtag gaagaaggct aagaagaaaa ataaatagtg gaggtataga
    18121 tatgcaaaaa gaaaataaag atgtttggct atatagttgg gatggtgaat gttttggaag
    18181 tgatgaatat gaaagcaaag aggaagctat tgaagctgct aaggaagaac ttctaaattt
    18241 tagagagttt ggagaatggg tttatgacaa agagtttggg aaattggttt atgttgggaa
    18301 aagagaagat gttagtcttc caggaataga tgtagaagat acattagaac gtgttcaaga
    18361 actgattgac gacgaatttg aagattatgg tgaagattgg ataacaagaa taaaagatga
    18421 agatagaaaa atacttgata acaaattgaa tgaggttttt tgcttatggg tagataaatt
    18481 tggatataaa ccgacatggt ttaaagttgt agaagtagaa aaaataaaac taaatgaggt
    18541 agccaatgaa agttaatttt acaatagatg gagaaccagt tggtaaagaa agaccgagaa
    18601 tgaactctat aactaaaagg acctacacac ctaataagac tagagattat gaagaactaa
    18661 taagatggct atatcaatct aaagttaaat attactttga aggttatata aaaatgactt
    18721 taagatgtta ttactctata gctaaaagta acagtaaaaa agttaaggag cagaaaagaa
    18781 ataatgtatt aagacctagc aagaaacccg atattgataa tgtggtcaag attatagctg
    18841 attcactcaa tgagatagct tataaggatg atacacagat tgttgaggtt gtagctagta
    18901 aatattacag tgataaacca agggttgaag ttatattgga agatattagt tgaacaacgg
    18961 aaaaatccgt ttatcaaatc attagataaa aataatttgg aggaggatta taagtatgaa
    19021 tgatttaaaa ttaattgaaa atgaaggact tataaaagta tatacaacag atgaagatat
    19081 aaaagtggta gatgggagag aactttggga aggattagaa gtagaaaaag atttttctga
    19141 ctggattaaa agtaacttag aaaatgtgga tgcagtagaa ggaatagact tttccacttt
    19201 aaaggggaaa acctctgaac agggaggaag accttcaata gaatatatat taaatttaga
    19261 gatagcaaaa gaaatttgtt tagtagcagg agcaagccca agagcaaata aagaacttaa
    19321 aagaaactct aaaaattata gaaaatatct aattgcagta gaagaaaaat acaaagtatc
    19381 aaacaatctt acaaaaacac agttaaatca aattaatgat attgtaagta acgcattgtg
    19441 tgaaatgcaa actaagcatg atgcacaaat agaacaattt aaaaaagaat cttcacaata
    19501 ttatagacca acctctaaaa ctaaatatga tatttcaagc tacataaaag atagattgga
    19561 tattcctaga gctaatgagg agtttgattt ggttaaaaag agaactttat taattcttgg
    19621 agctgataag tgggaagata taccaaaaga tgtgttatta aaatccctaa atattataga
    19681 tgaatcaata agaattataa aatctgaaag aaagacaaat cagattagtt tctttgaaaa
    19741 agataatttc tgttaataaa aagaaaaaag gagtgctttc acactccact tgtcaaaaat
    19801 ataaaacttt tatatacaaa tattattata acataaaaac ataaaaaatt gatgggagtg
    19861 tggaagtatg aataaaaaga cactatttca agaggttgaa ggtagattat ataactataa
    19921 aaaactagaa agtcaaataa gaataaaaga tatatatatt aaaaaattag aaagtgagtt
    19981 ttgcggatgt aaggctcaaa gttatgaaga aaaaacagga cctacttata atataagttc
    20041 aagtgttgaa aatgaagtta ttaagagaga agaagattta aatagattaa aagaagataa
    20101 gaaaacatta gaaattgaaa aagaaactat agaatgtgca ttaacaagtc taaatagctt
    20161 tgaaacagag ttcttcaatg aaatgtatat gaacaatgag aaaatcaaca tggattatat
    20221 gtctaatgct atgcacatag atagaagtca ttgttttagg attagaaaaa ggatagtttg
    20281 taagattatg gatatgttat atccgaagat aaaagagttt gaattaccca ttttttcatg
    20341 gaaagcttaa aaatgagact attttgagac tttttggaga ctatcgtgag actttttatt
    20401 gccaattatg tgagataata atatcgtgga aataaagatt tccctctcaa aacttaataa
    20461 ttgctaggtt agtttaaagg gctaatctag caatatgaac agactaggca gggcgtgagg
    20521 acgctgttag ttcaattcta actatgttca aatattaatc aacgtataca ctaaaagtag
    20581 agaaattgag ggcaaaattt tatataattt tgtatcttaa ttcaaagtct aaaaaccgag
    20641 tggggcttgg taacctcact caccatgcag gtgctggtgc ttaatctaag ttcgattctt
    20701 agaacttgca acataatata tgtacctccc ccttttataa aaagactaag ttcgggtaaa
    20761 cttagtcttt tattttttta caggagtatg ataaatgagt aatttaagag aaaagataat
    20821 aaaagaatta gatgaattta atatagatgc agatgatgaa tttttaagtt atggagtgga
    20881 atatgttgag agttttacag ggaaaagtgc tattagcgaa gagttattaa taagaggagt
    20941 agtgtttatg gcaaattata tagtaaatat ggaaaataaa aacttagatt aatttctagg
    21001 tcttttttat acaaaaattt agaacaggag gtcttaaatt gaacaaaaga gaagttgttt
    21061 atcttaattt agaagatata aaagtagata aacaagcttc ataataaaaa agtaaaaacg
    21121 aataccgagg tggtgatgtg gcaaaggcta gaagtccaaa cagaaacaaa gcttttgata
    21181 tatttaaaga gcataatgga aatattgcaa atagagagat agcagagata ttaaatgaaa
    21241 atgaaaaaac aataagcaat tggaaatgca gagataactg gaatgtagta ctacagaaag
    21301 ataaatgtag tactacaaag aaaaaaggtg gtcaaaagaa taataaaaat gctttaaaag
    21361 atgggatata tgaaaacata ttttttaaca ctctcacaac tgaagaaata gagttaataa
    21421 aaagtgagaa taaagatgaa atacacagac ttgaactaga aataagctta cttacagtta
    21481 gagaacacag acatatgaaa agaataaaac agtatgaaga aaaagttgat aaaattactc
    21541 tagaaagcat agataaaaga actttaaaga tagaaggtaa tttacttaaa gataatgagc
    21601 agacacagac agaaactgtc acaagagctg tttctatctt tgaaagagta caaaagctag
    21661 aaactgagtt aactaagata caaaaagaaa aagctaagaa tatagaaaga ttacaaaaat
    21721 taaaaatgga taaaaagaga tttgaattag agatagacaa taacaataaa gatgattcaa
    21781 aaacttgggt tgaagctata caaaatattg caatgaaacg tggtgttaac aatgaataaa
    21841 gctttattaa cactactaga ttgttattgg gataatcctg tttggtttgc agaggatatg
    21901 ttaaatttta aagctgataa gtggcaatct gatgttctaa tggctttagc tcaaaacccc
    21961 aaagtatcaa ttagaagtgg tcaaggtgta ggtaaaactg gcttagaaag tattgcgaca
    22021 gtatggtatt taagtactag accctttccg aaagtagttg ctacagctcc cacaaggcaa
    22081 caattatatg atgtactatg ggctgaaata gctaaatggc taagtaatag taaggttgaa
    22141 aagctacttg aatggactaa aacaaaagtg tatatgaaag gttttgaaga aagatggtgg
    22201 gctacagcta gaacagcagt aaagcccgag aatatgcaag gtttccatga agattacatg
    22261 ttatttgttg ttgatgaagc ttcaggagtt gctgacccta ttatggaagc tatattggga
    22321 acattatcgg gtgcagaaaa caagcttctt ttatgtggaa acccaactag aacgagcgga
    22381 acattttacg atagccacaa tagagataga gatttatata aaacatttaa ggtatcttct
    22441 ttagatagcc ctagaacatc aaaagataat attgaaatgc taaaaagaaa gtaccatgaa
    22501 ggttctgacc catggcgcgt tagagttctt ggagagtttc caaaaggtga aagtgattct
    22561 ttaatatctt tagaagctgt tgaaacaagc acaataagag aagtgaatat atctaatgac
    22621 tatatattaa atataggggc ggatatagca agatatggtg atgatgaaac cataatagct
    22681 ccaagaatag gtgggaaagt atttgattta ttaacttatt caaaaaaaga tacaatggaa
    22741 acagtaggaa atatattaag agcagttgat aaatttaaaa atatgtatca tcaaattaac
    22801 agagtaaaaa taaaaacgga tgatgatggc ttaggtgcag gtgtaacaga cagattaaaa
    22861 gaagttataa gacatgaaag acttaaatat gaagttatac ctattcaaaa tggttctagt
    22921 gctatagaaa aagataagta ctataataaa gcttctgaaa tgtgggataa catgagggag
    22981 gaattagatg caaatttaag cagttttata caaaataaag aagctataat acagcttcct
    23041 aatgatgata aacttattaa acaactatca aatagaaaat atacagtaga ttcaaaaggg
    23101 aaaatacaaa tagaaagtaa aaaggaaatg aaaaaaagaa ttggagaatc acccgataga
    23161 gctgatgcag taatatattc gtttgcagaa aataacaata ctgatttatc tttgctgaaa
    23221 gggggtagtg tatggggata atatcttatg taaaaaagct atttaaagga ccagcaggag
    23281 agattatgcg tatgtctagt ggaaacattg gcgtatataa attagacgat tctagagttg
    23341 attatgagtt agcaagagaa ctgtatcaaa ataaaaatgc taattacaag ttaggttcta
    23401 gttttgttag accgattgtc aattcaacaa ctggttttat gggtgtacct cattttcaaa
    23461 tagaagatga agaagctcaa tatatattag atgaatttgt tttagataac acatctaaaa
    23521 tgttaaaaac acatacagat agtttaaagc aaggtgattg ttatatttgg ataactagag
    23581 aagaaagaga aaatccttta tatcccgata aaaaagttag attaatatat aacttcatat
    23641 cacccgaaga agtgaaagaa ataatattag accctacaac aaaagagcct atagcttata
    23701 tattagaaag tcaaaatgaa tggactgact taggagaaaa caagagaagg gctaaggtaa
    23761 aacaaataat aactgctgaa agtagatttg ttgaggttga aggtgataag atagaaggtt
    23821 tagaagaagg ggaaacgcct aatgtatggg gttttatacc aataatacat tttaaaaatg
    23881 aagctgatga aacattgaaa tatggtcaaa gtgatataga gccaatagaa cctcttttaa
    23941 aagcttacca tgatgttatg ttacatgctt taaaaggtag caaaatgcac tctactccaa
    24001 aactaaagtt gaaattaact gatgttgcaa gtttcttagc acacaatttt ggtgttgaag
    24061 acccagttaa atttgcaaaa gagggtggaa agataaatct tgatggacat gaaatactat
    24121 tcttaaataa agatgaagaa gctgagtttg tagaagtaaa atcagctata ggtgatgcta
    24181 aggagctttt aaagcttctt ttttattgca tagtagatgt atctgaaaca cccgagttta
    24241 tatttggagt acatacacct agtgctttag cttctgtaaa agaacaaatg cctattatgg
    24301 taaataagat aagaagaaaa agagaacaat ttacaaatag ctggcaatta cttgcaagaa
    24361 tggttttaat aatgagttct aattctagtg gtatgaaata ttcaacttat gatgtgaata
    24421 taggttggga tgaagtaaat ccgcgagatg ataaagaatt agctgaaaca ctagaaaaag
    24481 tatgtagtgc attagataaa gctttagagg gtggatttat tagtgaagaa tcaacagtaa
    24541 attttctagc tcagtatata gatacaatga gcaattatat aagtgatgac cctgaaatag
    24601 ttggagaaag agaaaagata ataaaaacta aaatgttaaa atacagatta gatgattctc
    24661 aaggtctaga tgatgagtca aatgaaattg acaaggaaat agataaaata aaggataata
    24721 atgacaatgg ataaaagtac tttggaatta ataactgttg caggggagta caagaaatgg
    24781 gcattagaag ctagaaaaaa acttatagat ttaagactca agcaagatga tgaaataaga
    24841 acaatgtata ttaacataac aagaaatatt acaaaagaaa taagaaaagg aaatctttca
    24901 gactttaaca aagttaggtt aaaacagata ctaaaacaat taacacaaga aataaaaata
    24961 ttaaatgaac aactagtatt taattttgat gaatacttaa ataaaaatgt tgaaacagct
    25021 actagttact ctaaaaatat tttaattaat gcagttgaga cagctcaaat aactaaagta
    25081 actaaagcta tgatacaaaa agctttctat gatattaata taagaactgt agaagcttat
    25141 tatacaaggg ttaaggatgg tttattttta tctgatagaa tttggtctaa gtgtaagaag
    25201 tacagagaag atatgaaagt tatattacaa acagcagtaa cagaaggtca agactgtgtt
    25261 aaaacagcta agatgctaga caaatacgtt ttaaaaggta agaaaacttt agttgacgaa
    25321 tatccaaata tgataaaaag aataggaaat agagtacctc aaaatataag ttatgaagct
    25381 ttaagattgg caagaactga aatgacatca gcttatggtg atggggtttt agcttctgca
    25441 atgattaacc ctgcaaccat aggtattcag tttatgttgt ccatggcaca tcctcacaca
    25501 gatatatgtg acgaaatatg tggagaggat aattttggtt tgggtaaagg tgtttatcct
    25561 ataaatgaag ctcctgtata tccattccac cctcattgtt tgtgtattat gcttactgta
    25621 gttcaaccat tagatatatt agttggaagg ttgaaaaatt ggattaaaaa tcctatgaat
    25681 gatgtacctc ttgaaatgtg gtatcaagag gtgtatggaa atttgaattt ttaaattgaa
    25741 aggtggtgat taaatgaatg taataactgg agaaatggac tcaatgaatg cgttaatatc
    25801 tagtataaaa ccttctgata ttcctttagc taaagatata gacatagaag ctttaaaatc
    25861 tatagatgat gaccctcttg aggtagttgt tgagatacca gctacaaaat ctaaaagggg
    25921 atggaattat actgctaaaa gcttgaaaga tattgtagat tacactaatg aaaatactct
    25981 taatggcttt ttaggacatc aaaaagctga aaatatatca actgaatttg caccacctgt
    26041 aacgcattgg ataggtgcag aaatgaaagg ggataaagct tatttcagag ggctgattga
    26101 tgctgatgca acaaatttaa aaagatggat tagaactaaa aggataaaag aagttagtat
    26161 atttggttat ccaaaactta aaaagagtgc taaaggcgaa atgaatgtta taggatatga
    26221 gccactatct attgattgga ctcctctaca tagaccaggt atgccaacaa gtattgtagg
    26281 tatggaaatg agtcctaatg gcgaacagtt agatggaact tttgaagctt taagaataga
    26341 tttaagagaa gctttaaaag ctaagttttc tattaatgat aataattcat atctctatat
    26401 acaaaacata agatatgata acaatactgt catatatgag ttggagcaaa atggattatg
    26461 caagctttat agtataccat ttactatagt tgaaaataaa ataaatctag gtgaagaaat
    26521 tgaagtaata aagaaaataa gctatgaagc taaaggagaa atgaaaggag aggaaaacaa
    26581 attggaagga aaagagttaa taaaaaatgt caaaggatta ctgcaaactg gtgaaatatc
    26641 atattcagag gtcatacaag gaataggttt aactaaggaa attgtgacag gagagatgga
    26701 agatgtaaaa agttcattaa aagcagaaaa agaattaaga gaagtgaaaa aagtacttgg
    26761 aatagtagga gagatggaca cagttgaagt ggcaaaaaag gcttcaaaag ctttagaaaa
    26821 tgagaaaaag gaagcttgga actgtatagt taataaagta attaaagata aagtgtcagg
    26881 tgaaatagct caaacattag ttaagaaaat gttaaatgtt gaggaaggct caagtgaaga
    26941 agtaataaca ggagaaatag aaaatatatt aaatgatgag tttgtaaaaa atacaatgtc
    27001 taatatgtat aaagataatc caacaacaac aggattatta aactctagca ataatggaag
    27061 tttaacaact aagaaaaata gaatataaag gagtgatgtt tatatggcat ttaaaggtca
    27121 accaacgcca agcacaataa cacagataac aagagcaaaa ataagtgatg ggaaatctgt
    27181 aagagttatt ctttcagatg gtgaaagcac taaaacacaa caattttatc ttataaatgg
    27241 attctttgga gtcgctatgc aagacggaga aaaaggcgac gaagttactt tgcaaataga
    27301 gcaagctgaa tacgaaacgg ataatattgt tacatcagaa gcttttgagg caggggaatt
    27361 gatttattgg gataatacag ctaagaaatt tactactaca tctacaagta ataggctagt
    27421 tggtagagta acagatggga aagacagtaa taatgtaatt tggtttatat tattacctca
    27481 acaatagaaa aggagtgata aatatatggc atttaaagta attagtcagg aaaatttgct
    27541 ggaacaaaaa agaaaagaaa ctttacaaga agatatacca tttatagtaa atggtgaaat
    27601 ggaatatgta acaaagaaaa tatcaaatgg agaaatggaa accttggagt taaataagcc
    27661 acttggtgaa atgatgactt ttagctcgac ttcaaattta aaagagttat taagaaaagt
    27721 tgtattagat gttgaactag gcagagagca agtacaacta ttatataaac caatctatga
    27781 cagtatagca gattctaatt taccacaagt tatggatgct aagtgggctt tacaaggtaa
    27841 ctgtgtattc ctagagcata tagaaggtga agaaattaaa ttcggtacaa taaatgcaga
    27901 aaatggtcca gttgcaagga tacaaactta tgcaactggt tttgagtata caaaagaaat
    27961 gaaggatttt aaccaaacat ttagtgttga aatattaaat aaatcaattg gtgagagtta
    28021 caatgccttg ttaaaccaca tacatctaag cccaataata aattttaatt ataaagcttc
    28081 taataagaca gcttttaaag gtgaaactaa tgacccaata tggctaggaa tttggagaac
    28141 attaacacaa gcacaaaaag atacagttat agcaaaaaga caaggtaata tattaatggc
    28201 ttctagtgct gaccaaattg aaatagaaat ggcgttaaat ggaggacatt tattaaacgg
    28261 aagcatgtat ccatctataa aaaatatatc aacagtaatt tattatgatg ggtgggaggt
    28321 tactgttggt aaaaaaacat attcttacaa aggtgttaca ccaggcaaag gatatttggt
    28381 aagacctaag cgaggattta aagagttaat aaagagagat ttaacaacag aggttggaaa
    28441 tgctgattta agtaagttag tagaaaatca aattgtaggt cattgttata gaggtgcttt
    28501 tgcagcagta gaagaaaatg tacaagaaat aagttttaga taaaacactc ataagagtgt
    28561 tatttttatg aggtgataat atatgacacc aactagtaat ttaatagaaa aattaagact
    28621 attattaaat gataaagata aaaaatcatt tacagatgaa gaattaaact tgtttttaga
    28681 agaagcagac tgtatttact gtgcagcttc tcaaggatgg gtattaaaat ctttacaata
    28741 tgaaaataca gtaggggaaa tgtatgagta taaagtgggt caagaaacat ataaaagctc
    28801 tagtataaaa gacctagtat ctgtagctta tcaaaatgcg gataaattca aggatatgtg
    28861 tactaacaaa aaagaaaaag gaagttttat gttaggcatt agcacagaat ttgaaatatg
    28921 ataaatattg atagaagaag aaaagacata ataagaacta ttaatatgaa ccctaccaat
    28981 attactataa ctagtattaa aaaaactgaa atagatggag cttttgaaga aattgaaaca
    29041 gagataaaat gtactgttag aatatttaat gaaaagacag cagagaaaca aatatcaagt
    29101 gaaaagcaag gtacttttag ttctattaga acttatggaa tgttagtaag tagtgatgtt
    29161 gtattagaga ttaacagtag agatttttta gagtttgagt gcatatatgg gaaaatgaaa
    29221 atagttaata tatatcctca aattgtaaaa ggagaacttt gtgggtatca atgttcactt
    29281 gaaaggattg attaaaatga gtgctttcac aaatgcaata aatggtataa atagaaaaaa
    29341 agcaggtatg tttgtacttt gcatgagtgc aagtgcaatg ctagaaggtg aagctaaagc
    29401 aaatgcaagt tggacagaca gaacatcaca cgcaagacaa agtttaaatg ctaaaactct
    29461 tggaggggga aataatttca ttattagatt atcgcatggt gcagaatatg gaggaatact
    29521 tgaagaaggc tcaaaaccac atgttattac tccaaaatca gctcaagctt tatactggag
    29581 aggtgcttca catcctgcaa aatcagttca acatcctggt acaaaagcaa tgcctattat
    29641 aaaaccaact attgataaaa atataagcaa aataggtaat ataattttta gatattggag
    29701 tgattaaaat tgaaatatac tttagctaat attagacctt tgaataattt tttagatttt
    29761 aatttagaaa gttttgaaat actaacaaaa aaaatgccaa tcgatatatt aaagccaaaa
    29821 gtagaactta ttagccttga ttatggtgaa gtaaaattaa taaaaaaata tactgactgt
    29881 atttgattat ttggagcaaa taaatgagag caggaataag aaaagcgtta atagataata
    29941 taaaagaatt aaaaggttgt tatgaaccta atgtaccaaa caaagatact aaaaagcctt
    30001 atatggtagt tgtacaaggg caagacaatg acaacggaga aacaataggc tttgaaagaa
    30061 gtatagaaat atggatttat gaaggcagaa caacattcaa gaaattagat aaattaacta
    30121 aacaagttgt tgaagtctta gatatgaata ctatagttga tgaatctgaa aacgaagctt
    30181 ttacttgcat ttataaaggt acaagtgaaa atgatattgt tgttgaggaa tgggatgcta
    30241 tagcaagagg tataaggttt agtgtaatag ctttagaaga taaagaagat acaactaatg
    30301 ataggtgggt tgaagcttta tctaagtata caaaagattc attaggagtt gagagttata
    30361 aagataactg gaagaaaaac tttatagcac cttgttgttt atggagagct acaaatattg
    30421 aaaacaaaag aataaattat catctgattg agattacaaa aactatgaaa tgtcatgttg
    30481 ttagcaaaaa taaggatgaa atagttaagc ttcttgaagc attagaaaca agcttaataa
    30541 tagataagag agtaagactt agagaagata agaatatgta tttaactctt gttagtgtag
    30601 ttgaagatag ggaatcagat atgtttacaa ctggacaatt aacagctgta tttaaaatga
    30661 taggaaagat aaaaagagaa ggtcctacta tggataaaat ttatggtaat ggaaatttaa
    30721 aataggaggt gcaaggattg gctgaaacaa ataataaaaa gattaatgta agtaagcaag
    30781 aagaaaaata tttgaagagt gattttataa aaaatagcga agcacttggc tacaagaaag
    30841 aagtagttgc aggtgcttta tttaattgta agaaagaaga acttacaaaa tcagagtttg
    30901 agaaagcaat aaaagagttt ttagaaagag aggtgaaata gtatatggca actggaacat
    30961 ggaatgaaaa agagaaaaaa gaaatacctg gtttttataa caggtttaag actcaagcag
    31021 aaaaatctac aaatacaggt ttaaagggta gattagcaat gcctattaag gctaattggg
    31081 gagaagttgg caaggttgta acaataaaaa atgatttgag acaactaaaa acattgtttg
    31141 gtgatgatat gagctattca gcgtataagt taggcaaatt agctttatta gggaatgtaa
    31201 aggaattact tttatatagg cttgtagatg gaaatcaaaa gaagggtaca ttaacactaa
    31261 aagatactac agagaatagt gcaaaagatg taattaagct agaaactaag tatccaacta
    31321 gcagaaattt taatgtaaca ataaaatcta atctagtaga tgcagataaa aaagacttta
    31381 tattctttga aggaactaaa caattattta gttcaagtgt caaagggact atagatgaaa
    31441 tagtactaga aataaactct aatttagata atgaatatgt aattgcagca aaaatagcgg
    31501 atagtgatac aacactagca aacttagtaa atgtagcact tgaaggtggt aatgatggtt
    31561 gcacatctat tactaatgag tcttatctaa aagcactaga agaatttgaa agatatagtt
    31621 ttgactcttt tgtacttgat ggtgtggctg aggaagcatt gcaggaaact acaaaagctt
    31681 gggtagctaa gaataaggag ctaggaaaag atatattatt atttcttggt ggaaaaacag
    31741 aggataatat aaagcaaatt aatgataaat caaaaggttt caatgatgaa aatatagtta
    31801 atattggaag ctcagcctat tatgaaaata taaaatatac acctagtgaa gtagctgttt
    31861 acattgctgc tctttctgta agtaaaggta taacaggaag tatatgtaat gctaagacta
    31921 tatttgaaga agtagaacca agattaagcc aaagtgaagt aaaagagtgt ttgaaatcag
    31981 gtacactaat acttgacttt gatgatggag atgtaattat agttgatgat gtaaacacat
    32041 ttaagaagta tgtagatgat aaaaacgaag caatgggata tatctctaat atcatgttta
    32101 ttaatactat aaataaagat acttcattaa aaagaaaaga gtttgtaggt aagatattta
    32161 atgattcgac aggtcaaaca actgttatat gtgcattgaa gaaatatttt gaagaattga
    32221 tgagtcaagg tattatatca gaatttaatg ttgatataga tacagagctt caagcaactg
    32281 ccaaagcaga tgaattttac tggaagtggg atgctgttaa ggttgatgtc atgaaaaaaa
    32341 tatatggtac tggatactta ggataaagga ggttatagat tatgtataat gatgattata
    32401 tagaagaagc cagttttctg aatggttctg atgtagttat acttattgat ggtgtagaag
    32461 agctatacat ggaagaaata aaagctgatt ttgagcaaga tgagcaaagt attaaactgt
    32521 tagggtgtca aaatgaaata tcaagggttg gtactactaa aggttcattc tccttgaatg
    32581 gatataagac agattcaaaa tttgcaaaat taggatttag gtcttttgaa ataatatata
    32641 atttatctaa ttctgaaaca ttaggatatg aaagtattag attaaagaat tgtagattaa
    32701 aaaaattgcc tcttataaat tctaaagctg gtgaaattgt aaaaatagaa gtagagggaa
    32761 gttttagagg atatgatttg ttaaatgaac tttaaaacaa aataatgttg tactttaaag
    32821 ctatgaacaa ttaattttgt ttgtagcttt ttaaaattaa aaatattgga ggaatagtat
    32881 gtcagagatg tataaaagag agttagaaaa tgaggcaata gaaagagaag ataatgattt
    32941 agatgaagaa atagaagaaa caaatgaaga tagattgaag atgaaagagg atgaaataat
    33001 agcaaagcta ttggaggatt caccagtacc tgagagaact gtgtttttag atagattagg
    33061 aataccaatc acattaaaag cattaacaga aaaagagatt agtaggataa gaaaagattg
    33121 tactaaaatt gtaaaggtcc aagggcgaag agaagaaaaa ttaaatgatg atgagtttac
    33181 tctagcatta atagaaaaag gaactgtaaa acctaatttt tctaatcaaa aattacttga
    33241 tgctatgaaa gtaacaaatg ctagagaatt tataaagaga aaattcttag ctggagaatt
    33301 aagtaaaata agtgaccaaa tattagaatt atccggtttt tatgatgaaa taagtgatga
    33361 tgatataaaa aactaataaa aatgggggga cgactgactg ttttaaataa catctttgtt
    33421 aagcatcatg ttcccccaga tatttatgtt aagaaaaatt taatgtctca acgcttaatg
    33481 aaagtattta ctcaaaatga aatagagcaa gagaataaag ctatgaaaaa ataaatatct
    33541 acagaaaggt aggtgaggga aatagctaaa aaggaaatgt atcatattga tgttgtcata
    33601 agtgcaaaag gcgacggaga aacaaaaagc aaactaagtg caatggaaaa atacatgaag
    33661 cagacagaaa aaagaatgca aacacttaat aggataaagg ttaatcctgc tataaaagct
    33721 actgataaag cttcaagtgt tgtaaataga gttaataaca atatgaataa agcaaaaaaa
    33781 actgttacag ctagaataaa agctacagat aacgcgagtc cagtagccaa tagagctagc
    33841 aataatgtta ataaagctaa aaagacagta acagcaaggt tgaaagctac agataatgcg
    33901 agttctactg ttaacaaagt taataataag ataaaagaag ttgctaagcc tgtacctcct
    33961 gtaatcatac gaggacaaga tgaatctagt tctataatag ataaagtaaa agctaagatt
    34021 cagaatctaa aagctgatac tatcataaaa ataaaatcac aagctgatga agctataaat
    34081 actatttctc gaactaaaaa taaattacaa gaatttgtga gtaagagata tgaagcagca
    34141 gttaagatta gagatgaagc tagttcagca cttagagggc ttacaggaaa aatagattct
    34201 tttgtaagtg gagctattag taaattcgct agactggcta ctaccgcagg agctttaata
    34261 ggtggaattg gtgtaggttc tgctgtaaaa ggatttgcta cttttgaaca aagtatgaag
    34321 aatacacaag ccgtaagtgg agcaacagga aaagaaatgg aagctttaac tgcaaaagct
    34381 agacaacttg ggagagaaac tagttttaca gctaaagatg caggagacgc aatgtattac
    34441 atgggtatgg caggatggaa gtccgagcaa atgataaaag caattcctga cgttcttaac
    34501 ttggcagcag caggaggaac agacttagcg ctaacgagtg acattgtgac tgatggacta
    34561 actgcattag gaatgactgc aaatgacaca actgaatttg ttgatgtgat ggcagcaaca
    34621 ataactaatt caaatacaag tgttgagtta atgggtgaaa catttaaata cgtaggctct
    34681 atgggtggag cattaggagt atctatgaaa gatttatctc ttgcgacagg tctaatggct
    34741 agtgcaagtg tcaagggaag tatggcaggt acttcgctaa gaggaggtct agttagatta
    34801 ataaagccac cggccgaagc agcttctgca atcaaaaaat atggaataga attaaagaaa
    34861 aataaagatg gaagtttaga tttagcagga actataggaa gtttaagaga aaaacttggt
    34921 ggattagaaa aagttgaaaa aggagctgct atacaatcta tttttggtcg tacagctatg
    34981 gcaggttggg cggctgtagt aaatgctagt gaaagtgatt ttaataagtt aactacagca
    35041 atagcagaaa gtgaaggaga agctaagaga attgctgata tgaaactaga taccctgtca
    35101 ggacaatttg aaattttaaa aagcgctatt gatgatgtaa gaatatcggt aggaagtaaa
    35161 ctaggtccta tgactcgcca atttgtagaa gatttaatta agaaaatgcc acaaatcggt
    35221 gatgctatag ttggagtagt agaaaagttt gttaataatt tcgataaaat aaaagcagga
    35281 tttcaagtat tattgcctgc aattggttct gttatagggg ctattatagt tctcaaagga
    35341 gtttttgctt ttggaagtgc aatcaagagt ttaagtcttt tagctaatgt ttttggaaca
    35401 gctaaattag ctgctcttgg tttgacagca ataataggag ctgtagttat agcttttgca
    35461 ggaatgacag tcgcaataag caataacaaa attgctataa tggatttaca aactaggttt
    35521 ggttcatttg gcgagtatat tactacaata atggagagca ttggtggagt cataaaactt
    35581 acattaggta acttgcttat aatgttaggt ggaattggaa aaggaatagg catattgtta
    35641 tctgataaga gttgggatga aaaagcttca tcattaaaaa atctatttgg taaaacaaca
    35701 gcagaaatta aaacaaatac aaaagaagct ttatcagata taaattcaga aacttctaac
    35761 gcaacagctc tattaaaaaa gtctacttca aaagagttac aaggtgttac taaagctttc
    35821 tcaacagctt ttgaccaatc taaaaatgta acagagaaga aatctagtga tatagctaga
    35881 gcattaacaa atggattaaa aggtttagat gaccaatctc ttactatgat gcgaggatta
    35941 aatgataata tggcgataat tttatctggt gtgacagcag atatgaaacc tagtgacaaa
    36001 gttaacaaaa tcaccaaaaa tcttgatgat gctttcaaag caggtaaatt aagtgcacaa
    36061 gaatacagaa gtagtataca agaaacttta agttttataa gtaaatatag tgcagattct
    36121 tcaaataatt taaaacaagg catgagtgat gctttcaatg ccttcaagga agggacaaat
    36181 gtaggtggat taaaagacgg agtaacagga atgttgaact ctttgaaagc gacaggaccg
    36241 caggctttag aaaccttaaa aagtttagga ggaaaagcaa gcgaaatttt taagggagtg
    36301 gattttaatt cttctataga tgcacagaaa actaaagtat tacagaatct aaataatcta
    36361 ggtttggaag gaacacaagc tatagataca ttaagaacta tcttctcaca ggcttctact
    36421 gcactagata ctacaaattt gaaacaaggt ttaagcgata catttaattc atttaaaatc
    36481 ggatttaatc aagatgggat aaatggagca ataaatagta tgcttggaac gataaatcaa
    36541 ataggaccac aaatgcaaga agctctaggc aaaatggatg gaaagatggg gcaagtattt
    36601 gctaatgtag actttagcac cccaatagaa acacaagctt ctaaagtttt ggaaaatcta
    36661 aaaaatttag gaatagaagg accacaagct ttagaaactg taagaagtat atttgctcaa
    36721 gcttcaagtc aaatacaagg ttcagcttct caaactgctc aacaagcaaa tcaacaggtt
    36781 gcaaatgctc taacacaaga aaacccagct gtgcaacagg cagggcaaca attaggtaca
    36841 gatttgacaa atggtgtagt aaatggaatt caagcagggg tgccagccgt tcagcaaaaa
    36901 gggaatgaat taggagtagc aactcagcaa ggtgttacag atgctttaaa taatgcaact
    36961 cctcaagtca ataattctaa ccttacaact ggtatagata cggcatttaa ccaagctact
    37021 gcaacagttc aacaaggagc gacgaatatg tataacggag ctaaacaaag ctttagtcaa
    37081 ttagctcaag ttggaaaaga agcaggaagt agcttgtata atggtgctac aacttctttt
    37141 aatatgttag catcaaatgt aagggttgca tgtagtagca tgtacaacgg agctagaaca
    37201 tcttttacga gtttaagtag ttcagctata agtgtcattt ctgctatgtg tagttctgta
    37261 gtaagtcaag ttagtgctat gtcaagtcaa gttataagct attggaatag tgtaagagca
    37321 acagtttcag caccaataag tgcatctttt aatgtcaaga ctacacaaac aacagttaaa
    37381 aggacagtaa atgaaagtag tggtggaata ctaagtaata tatttggtcg ctttgcagaa
    37441 ggaggagttg caagtaaacc atctatatgt ggagaagatg gagctgaaat ggttattcct
    37501 ttatcaaatg ctagaagaaa tcgagcaata ggcttgtatg aacaaacagg taaaatgtta
    37561 ggattaggct caactccttc tcaaaaagta gtaggtagtt ctaatgttgt aaataacgtt
    37621 agacaattta caaatatttt ggattcagac aataaagaat ataaagaagc tcaaacacag
    37681 agtttagttt caagtaatgg gaatacaata aatttaggtg gaatatctat caatgttcaa
    37741 aatagtgata ataaagaaga aatgatacaa gaaataatgt ctcaagtaga aaatgaacta
    37801 agagaagcat tgcaagacat tggataatgt cgaattgttg ttaaaaaaat cttcctcata
    37861 aatgatataa tattgttaaa atatgcaaat ttatgagggg gaaattttat gtggcagaaa
    37921 tttaaaagtc taaatacttt cttaaaagtt attataggta ttatagcaat atgtttctta
    37981 ccaatcactt tagctgtttt ttcaattgaa tttttaatga aagctattga agctaaaaat
    38041 aaaagcaaaa taatcttagg atgtttttta gcattattaa cattaagttg tgctaggact
    38101 gtttatctta caagagattt agatgataaa aattctactc aagtcaatca agtttctagt
    38161 aataaagata aagaagatgc tgaaaagaaa attaaagaag aaacgaaaaa acaagaggaa
    38221 gttaagaaaa aagaggaaat agagaaaaat tctaaagagg acaacaaagg aaaagtggaa
    38281 acaaaaaaac aaacattaac tggtgaagag ctaaagaaaa aagttgattc tataattcca
    38341 gcagagtata aaggaaatta ttacacaaat gatgtattag atacagatgg tagctatgta
    38401 cttagtttac aagttcaaaa tgcaagtttt gacaacgaaa gcagttgtaa ggtatttact
    38461 aaagacttaa ttaacaagtt gaaagaattt agaatagact cagcagaaat atattttgta
    38521 ggttcaagtg gtcaaacaac atatcaaatt aatatagatg atttcttgaa agttcaagat
    38581 aatgttaata gcgttgataa catggaattt ttctctttca aagactttaa aaattagata
    38641 gacaagaaaa aggcatttat ttattaggaa tagatgtctt ttttattgta aaaaggaagt
    38701 gataacttgg taatagacat ttatctaaaa aatgaaaaag aaaagataga ttttcatttt
    38761 ccagtaaccc cacaagattc tctatctata aaaaaagaaa aaagatttga aactgtagat
    38821 atagtaaatc taggtgaatt tgacattaaa aaagaagggg agaagataag agaaatatca
    38881 tttaaaacat ttttaccttt tcaatatgac gtttcttatt gcagatatag tgagttaaaa
    38941 aatccaactg aaacagttgc aatgcttgaa aaatgggtag accaagctga acctctaaga
    39001 cttattataa ctggttttgg ctacaatgga ttagttacaa tatctagttt tagtaatacc
    39061 caaacagcag gaagagaaga agacagagac attgagataa catttagaac ttacagagaa
    39121 ctaaaaatag agacattaaa aaaagaaact aaaagtaatg ctaaaacaga tttaaaggac
    39181 aatagaccta acactcaatc atcatcaaaa acatatacag ttacatctgc agacgcatta
    39241 tggagtattg caaagaaatt tttaggtaag ggttcaagat ggacagagat ttataatata
    39301 cccgaaaaca aaaaagtcat tggtaaaaat ccaaatgtga ttaaaaaagg acaaaagttg
    39361 gtgatacctt ctaaatgaaa ataatattaa acggaaaata tgatattgca aattttaacg
    39421 agggaataac tctctcagaa gctatagatg gagttgcata taagatggat atatcattag
    39481 tagaacctaa acaacttcaa gacataggaa ttaaaaaggg tgataaaata gttctaattg
    39541 atatagcata tgagagtaaa aaagaagaaa caatcttcaa tggagtcata tgggaaacta
    39601 ggagaagtga aaagagtaaa aaactgacat tgtcttgtag agaaagaaca gtttacatgg
    39661 aagaaagtga agaacaatac tcgtttaaag aaaatacagc gacacagagg attgagtact
    39721 actgtaaaca atggaatata ccttattaca acttagctaa tacagggaag aaacttgcta
    39781 aagtaataca taagactaat atactagata tgattaaaaa agacttaaaa gaaacagcaa
    39841 gtaaaggtgg agatttattt agggtaagaa tggacaataa attaaaatta tttaagcttg
    39901 gtactaatgt aaatgtatat aagctggata gtatattaga agatgcaaac tttacaagta
    39961 gttttaatga tgcagtaaca agtgtaaaag ttttaggaaa gagtaaagat gaaaatacaa
    40021 aagcacctat aattgggaca tataaaaaag atgctgataa gtttggaact ctacaaaaga
    40081 ttaagcagga tgaaaagata aaaaatgcta aagaagctaa gaaagcagca gaagcaatgt
    40141 ttaattcggg tgaagaaaca ataagcgttg attgtgcagt ggatataaat aggatacgtg
    40201 caggtgataa ggtatctcta aaaggtaaag agtattatgt tatagatgtt actcatacac
    40261 tagattctaa accgaaaatg aagctcaata tagggacttt agaatatata aggaggaagt
    40321 tttatacaaa tgactgatgc cagatttaat ggagttgcta gaatattgaa agaaaaaatg
    40381 aataaaagtg ttaatgatgg agtttttgga ataggatgcg aacttgcaga aataacagca
    40441 aatgggctta aggttaatgg ttataaagat gaaatacagg attatctagt attagagaat
    40501 ttaactttga aagaggatta ttttactctt tcagatgaaa tgttaggtgc agaatataga
    40561 cataaacata aaatagaaac tccaaaagaa ttaaaaccct tggctatagg cgataaggtg
    40621 ttagtagccg ttatgggagc tgaatttgta gtaattggga gggttgtaaa tgccaaacct
    40681 atttcctcaa agtgaaactt ttgaaactgt agaattaaaa aataataatg agaatgaact
    40741 agacctaaag ggttcttttt tatttgattt tataaaaggt gaatttgtca aaaatgcaga
    40801 tggaacatta aaaaaatgcg ataaagttca agcatacaaa caatggtgtc aaaaggctat
    40861 attaacacct aggtacaaaa ggtcagctta ttctagtgta tatggaagtg aaataaaaga
    40921 attaatcgct agtaacttat ctaaaaatgc aaaagagctt gaaatatcta ggttaataaa
    40981 agaaactatt ttagttcatc catatacaaa agaagtagga gaatttaatt ttaactggtt
    41041 ggagaatagc aggttagtag agtatgaatt tgatgtacta acaatagatg atgaaaacat
    41101 aaccatagat gggaacataa aaggtaggtg attatatgga aagagagcta cctataccaa
    41161 tttttttaac agaagatgag gactctgtac atgaaaggat gttaagtaat tttcaagatg
    41221 tgagtacttt ggagggtgac tttatctatg atgcaacaag acctacagca gagcagatag
    41281 ctgaattaaa acaactaggg ctacaaaata atttaaagat agcatttcct caaactagtt
    41341 atggaactta cttagagtgg cttggtgaat gtaaaggtgt atttaagaat caaccaacta
    41401 aatcggttgg tatgattaca tttacaggtg tacaaggtac tataattaaa aaaggaacta
    41461 tattaactac tgttgcaact gatgaaaaac agagcataga atttgagctt cttgaaacta
    41521 aaactatagg agaaaatgaa acagtagata ttaaagcaga gtgtagagtt gcaggaatta
    41581 cagggaatgt gtctaaaggt agtgtatccg ttttactagg ttctattaat ggtattaaat
    41641 cagttactaa taaagaagat ttcaaaggtg gaacagatat agaagatgaa gaacatttta
    41701 gagaaagagt tcttgtagca gagcaagagg acaaattaag tggagctagt tcagattata
    41761 taagatgggc taaagaagtt aatggagtgg gatatgctta tgtagtttca gaatgggctg
    41821 gaccaggtac agtaaaagta ttaatactag ataaaaatag gaaagcagca acacaagaat
    41881 taatagataa ggtccaagaa tatatatatc cattgaatac agaagaaggt caaaatcgag
    41941 atggaaaagc tcctataggt gctattgtaa ctatagctac tccacaaaca ttactaatta
    42001 atgtaaaagc tagttttgtg ttttcaaatg gatttagtca agaaactgtg ctgaatatct
    42061 taaaaaataa aatagacaaa tacctcgata aaatagatat tggaggtact gtttcttata
    42121 atgcaattta ttcactaaca ggttctatga tgcttgcaga tgaaggaata caagattttt
    42181 ctaacttgac aataaatgat ggaacaacaa atattatttt aaatgaccaa gtcgttggaa
    42241 taggtgagat aatcaatgag gtgatagcgt gataacttct aaaaaaggta gagaaatgct
    42301 tttaacatta tctcctatct atgaacaatc tttagtcatg aactctatat atgaagctat
    42361 aggaagcgaa tttgataatc taggattatt aaataaagaa atagagttac aattatttcc
    42421 tcaaactgct acatggggac ttgaattttg ggaaaataga gtaggattga ttactaactt
    42481 agatgaagat atggaaacta gaagaagaaa agtcattgct aagcttcaga gtaaatatat
    42541 tatgacacct aagcgaatgt ctatgatatt gcagtcttac acaggtgcgg atataaaaat
    42601 aaatgaaaac atatccccat atacttttag tgttgagcta actagcactc aaggttttcc
    42661 tagagattta gaagatttat ataagagagt aaatgttata aagccttctc atttagctgt
    42721 aagttataag ttagtatctt tattgaaaag taaaacctat tttgcacaaa cagcaattat
    42781 gagcgaagaa ataactgtat atccttacac aagtaaagaa gtaaaagcaa gtgttaaagc
    42841 taagtttgca ttagctcata acatgagttc agaaacgtta acagtatatc caaaaggagg
    42901 tggtacgaat ggctgatgaa caattttaca caatactaac aaacataggt aaggctaaga
    42961 ttgctaacgc aggaatgtta ggtaaatcag tggtactaga taagattcaa gcaggtgatg
    43021 gaggaggaaa ctattacaat ccaacagaag accaaacagc attaaaaaat aaagtttggg
    43081 aaggtaatat aaatgctttt gacaaggatg aaaataatcc taattggatt attgcaacag
    43141 catgtgtacc tggttcaata ggtggattta cagttagaga aatggctctt atagataacg
    43201 aaggcgatat gattgcaatt tgtaaaagtc ccgaaacgta caagccaaaa gttggtaatg
    43261 gagctatgaa agatttatac ttgaaattta tcatagaagt atctaatgta gagaaagtga
    43321 cattagttgt tgacccaact gcaatatttc tgactaaaaa agatgaggaa aaaatcgtag
    43381 caagtatcag taagttagac actaaaatag atacaactaa aacagaatta aaaagcaaca
    43441 tagaaactgc taaaacagaa attgacgaga aaatagggga tacgacacaa cttactacaa
    43501 cagataaaac aaatatagtt agtgcattaa atgaggtaaa aactagtgta gatagtatag
    43561 aaacaacagc agagaaaaca agtataaaag atacagataa tttattcgaa agtgataatg
    43621 tagagggggc attaaaagag ttagctgaaa attataatac attatcagaa aaacagaata
    43681 atttagaaac agaagtaaat gggcagagga ctaaaggcat tactatagtg aataatttaa
    43741 tagatatgat ataagcgagg tgaaaatatg acagaaaaat taactgaaaa tgctagttta
    43801 ggggaactta tggcagcatt agaaaatgta caaactgatt ttcaaactgg taaaaataat
    43861 atatctagtg cattgggtag tccttttatt ggaacagata aatttggtac aactaaaaca
    43921 aaaatagaaa cattaaaaaa tgtattagtg gagacgatta attctaaaaa tgtttcagca
    43981 acatcatctg aaacatttac taatttgatt gaaaaagtta actggatttt tcaatcaata
    44041 gaaatttttt ctttaaaaaa tagaattcaa gctacaactt taaacactcc tagcatcgtt
    44101 tacaatgaag tatctagtat aaaaggcaca ttaagattca caggcgaact taaagcgtct
    44161 aaaatgcgtg ctgattatgc aacagcaaaa atagagatac tatgtggaaa ccgaaaagaa
    44221 tacttttatg ttactgacga tactcctagt gcatcttctt cttttgtaag atttacaaaa
    44281 gatattattg ttgagaatag catggatata aaagtacaaa tattattaac atctgtagga
    44341 gcaggaaatt tagatggttc atatgcagct cgtgcagaaa tagaaaaatt aacaatatta
    44401 aggtaggaat aaattatgaa aaaacaagta tattataatt ctttagatga aaaagaaaaa
    44461 ataattagtg aaaactctaa tttatatgtt atagaaatat atgaaacttt aaatgaaaat
    44521 tatttagtgt tatcaagtag tccaatagaa gatgaaaaac ttagttatga agaattagaa
    44581 aatgaattat taattatgac taatgaatta caaggaggat tgttataatg aatataaata
    44641 atgttgtggt aagaatatta gcagagagaa tattaaataa gggattaaat cctctaaaaa
    44701 atagagtatt tgaacttgat gatgttacta acacagagta caggatggca gttgaaaact
    44761 acattataga acatagtgga gtggtagaag gaacagaacc tgcaaaatag gttctttttt
    44821 aatatcaaaa attaggaggc ttagatgaac gaagaagtta taaaagaaaa aataaaacga
    44881 aatgaaataa gaataaacag acacagtgat gaaatagacg aattgaaagt agcaaatata
    44941 gagtctaaag cagaattaaa agcattgtgt gagaacttaa actcacttac aagtatgttg
    45001 aaatggctaa ttggaacaat gattacaacc ttagtagggt tctttatatt tgcagttcaa
    45061 agaggaatat tttaattagg aggaaaataa aagatggata atttaataag ttttatacca
    45121 gagcagttgc taattttagt agctgctctc tctattatag gtaaaggttg caaaaaatat
    45181 aaacaattag acaataaata cattccaatt atattacttg tgttgggaat cggattttct
    45241 atttggatgc tagggtttag tcctaacgca gtactgcaag gtgtaatttg ttggggaata
    45301 tcaataggta taaatcaaac ttacaaacaa ctaaaggagg aaaataaaca atgaaaatag
    45361 caatagtacc agggcatact ttaacaggaa aaggaactgg agcaactgga tatatagacg
    45421 aaggaaaaga aaatagaatt ttaactgatt taatagtaaa atggttgaaa caaggtggag
    45481 ctactgtata tactggaaaa gtagataagt ctaataacta tttagcagaa caatgtgaaa
    45541 ttgccaatag gcaaaatgta gatgtagctg tacaaataca ttttaacgct gaccatacaa
    45601 cattaaattc tatgggtaca gagacaattt acaaaactaa taacgggaaa atatacgcag
    45661 atagggtaaa tactaaatta gcaactgtat ttaaaaacag aggtgcaaaa tcagatgtca
    45721 gagggcttta ctggcttagt catacaaaag ctccagcgat attaatagaa gtgtgcttcg
    45781 tagatagcaa agcagataca gattattata ttagacataa agacatagtt gctaagttaa
    45841 tagcagaagg tattttaaat aaaaatataa ataatgaggg agttaaacag atgtacaaac
    45901 atacaattgt ttatgatgga gaagttgaca aaatccctgc aactgtagtt ggttgggggt
    45961 ataatgacgg gaaaatacta atatgtgata taaaagatta tgtacctggc cagacgcaaa
    46021 atctttatgt cattggtggt gcagcatgtg agaagatagg ttctattact aaagaaaaat
    46081 atactatgat aaatggcagt gatagatttg atacacttta taaagcatta gattttattg
    46141 atagatagaa atatagatat taataccttg tactttatat ctatatttta tttacagttt
    46201 gctaactata taataagcaa aaaatgatat attagaaata tataagttgc ttgcaaaaat
    46261 aaaactaaga tggacataag ttataaattt aattgtaagt ataaatacta caaaatagaa
    46321 aataattaga ttgaataaat tggttgcata tgcagaatta tttattatat gaaaatataa
    46381 agttatgcta tatttttata acttactcta tttataaaga cagtatatgg aaaaaattgg
    46441 ttgaaaaaag ataaaaccaa tattattata atagataaga gggtataaat atgatttatt
    46501 gtcatatttt agaaatttta ttatataaat ataatgtttt aaaaatagag gagttatatg
    46561 atgaataaag aattagttta tggtgagatt gatgaaaaaa atcttgatgt atcaagaata
    46621 agtcatgaag aattaaaggc tctattcaat ttaatgaacg ggaaacctga tagtatgctt
    46681 agaatttttc caagagatgt aaatgtagaa ataaaagatt tatattcttt acatgaaaaa
    46741 ataaaagaaa aattacaaca ttatatagta gcagctttaa tagtgaatat atcagttaag
    46801 tttgataaca atactttcaa agagttttct gattggatag aatttcaaaa ttgtaattgg
    46861 tctagctccc actatttaga aaatataact ataaagtatg atttttttgt tgattttaaa
    46921 gaatatgggg ttcctcagag acatacatta gtaataagac tatcttcagg aatgaagcct
    46981 caacagatat tacagctcat agtaagtggt caaatagaag aaatagattc tatagataaa
    47041 agcttatctc ctgttacttg caaagttgat tttactaatc atctattagg tgatgaatta
    47101 ataaatataa tagaagaatg ggataaatca atacaaaaag tagtttctga atcaggaaaa
    47161 acaatgaaat ttattagaaa atatagaaaa ggtatttctg ctgttataga aaatatcata
    47221 ccactttttg gcttaattgt tttggtaagt atagttaatt ttaatttgtc gcattttaat
    47281 atagatactt tagttcagtt aagcataagt catatacaaa aattaatcat gcttacattg
    47341 ctttttagtc ttatttataa agtaataaaa aatattggag aatttttatc aaataagttt
    47401 ttgacagaaa tttatgagta tggtgacact cacatattca acataacgaa tggagataaa
    47461 ataaaacgag agaagttaga tatcaaaaac aaaaaacatg aaaaaggaat aaaaagtagc
    47521 ttatcattaa cgattttaat taatgttatt tgtggaattg tggcaactta tatatgtact
    47581 attttttaat atttgagtat aattttaatc tattaagttc ataattatat aaaaatatat
    47641 tttaaattgt atttatagct acaaaattag ggggtgtgtt ttatgataaa aaaaataatt
    47701 gtatttttta aagaaagaaa acaaaaaaaa gaatatttag aagaacaagc tttctcaaat
    47761 aagtatattt cttatagtga ctataagtat ttgttagaac aaattaagta gtaaaatttt
    47821 atgaatttta aagtataaaa gctatagcaa tagtttttat actttttgtt tttatgtcta
    47881 tagctgtaat aataaaacga acgaaaatta tcagactaaa acataagaca tgctataatt
    47941 gtattagata aatgtttgaa tatataccaa aagtactctt tttataagag tgcttatttt
    48001 tttgaaattc atcaacatat aaactatcaa gaatattact caacacacct taaaatcgat
    48061 ttaaattctt tttcatacac aaagttatat gatataataa aaaagtaaat atgtaacccc
    48121 aacacatctt tactaagtca aacattatta tatagagcat tcttcattat ggagagtgct
    48181 ttttcatttc tttgaataac catgttgatt atttataata ttttcattta atttatcttt
    48241 atctatcaaa tttaatgcac aattaataca aatgttagtt ttcatatgac ttttcttatg
    48301 aaaagtaata tacttattat catctttgtt tattccttta ttacagtaat cacataatat
    48361 agtcttagtc atatttttat tcctttcatt ttatattttc cataattaag tttaacatat
    48421 ttagtatata cctatgtata tatatttaaa acaatgtttt ttattgcaat atagaatata
    48481 ttgtataaat aaaaaaatat ggagaatgga tatttgataa ttgtaaaatt atgttataat
    48541 taaaatgcaa gaatatatct tgcaagaggc tacaatctaa gggcgaaagt tttcggaaat
    48601 caccttcgaa cgaaaggggg tgagtatatg aaaacactta tgtttatact tgttatagcg
    48661 tattacatta ctattgtaat aaaatctatt acaacaatag taaagtctat aacaagatta
    48721 ttagattcta taagtagatt aaaagaatct ttacataaac taagaaatcg tcctgacggc
    48781 aatcaagacg atttcaatga ataaatcatt acttaaatta ttttgaattt gaaacttcgc
    48841 tctaggctaa tagattgtag tttctttttt tgtttttgat acgaatttac atctttatta
    48901 tatcgcattt taagaaaaaa taaaactatg aatattataa atctatttta attaattttt
    48961 tatcatatca ttatttaaaa gtagataaaa ctaaaaaggg ttctctagaa atcaatgtct
    49021 atctaattat acttacttac
//
LOCUS       DAEORZ010000009        48433 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEORZ010000009 DAEORZ010000000
VERSION     DAEORZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48433
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.009"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            109..1326
                     /locus_tag="KRH39_000483"
     CDS             109..1326
                     /locus_tag="KRH39_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBG8510411.1"
                     /translation="MLIGLIAAFGLVLQKKKASDVIKGSLLAAFGIIIMETGTGMLVS
                     SIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAIHLMIARFTPIKTIF
                     LTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPWLLRKYVFDVIGDDS
                     FTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREISISGGMAVFLMFIVV
                     GIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQIMPAFQGISEKVVPN
                     ALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNLLLIPLVITSFFECG
                     AAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNWILIFGGNDLSLFGI
                     ISRFIGSLFGGIL"
     gene            1329..2069
                     /locus_tag="KRH39_000484"
     CDS             1329..2069
                     /locus_tag="KRH39_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG8510412.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVGPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            2410..3702
                     /locus_tag="KRH39_000485"
     CDS             2410..3702
                     /locus_tag="KRH39_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG8510413.1"
                     /translation="MEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSKKDGEKE
                     KLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISAGEGPDV
                     GYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAANPGVLY
                     YNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFGNLNWNW
                     YPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEMIESVFG
                     PGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSSAKNKEL
                     AYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPLVVGPHG
                     VEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            3800..4717
                     /locus_tag="KRH39_000486"
     CDS             3800..4717
                     /locus_tag="KRH39_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG8510414.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            4733..5542
                     /locus_tag="KRH39_000487"
     CDS             4733..5542
                     /locus_tag="KRH39_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG8510415.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            5600..7348
                     /locus_tag="KRH39_000488"
     CDS             5600..7348
                     /locus_tag="KRH39_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBG8510416.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDMLVEQYLPQTFWVEGQVPEDFEENNFNIGIDIFKSFGYEDEEKACTIDVP
                     VEVKNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIIDNYLKELASLGEKV
                     ATIIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVSTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            7609..8631
                     /locus_tag="KRH39_000489"
     CDS             7609..8631
                     /locus_tag="KRH39_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBG8510417.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGVTKEAPETSRRAWVKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            8636..10942
                     /locus_tag="KRH39_000490"
     CDS             8636..10942
                     /locus_tag="KRH39_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG8510418.1"
                     /translation="MKKNSKKILITLTIITNIIYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKNIYPKEIELEINEGIVLECDLCEGKIDERGILYRVNNIDSIMQNFFLRGEMMDWI
                     LQNKTIFVSKPSEKKEKSIDEFEPMEYI"
     gene            10914..12029
                     /locus_tag="KRH39_000491"
     CDS             10914..12029
                     /locus_tag="KRH39_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510419.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSAKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVKIETNSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSVQSKNMLEFRLKEGSEIFLEDKNIVYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEKSNVVLDSNMLSNIASWGITFIPSDEEHNIVIIKKISLLSKEKL
                     GFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAKPGKIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVSPEQSPTVQDIK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            12047..13465
                     /locus_tag="KRH39_000492"
     CDS             12047..13465
                     /locus_tag="KRH39_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG8510420.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIASIIMNYIFGLLVDRYRESKIKVKIFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELINTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTMQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWIFFIFAFIFSVPIAKKINKFVVEQAKCSVIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            13481..14689
                     /locus_tag="KRH39_000493"
     CDS             13481..14689
                     /locus_tag="KRH39_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510421.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNICYSGKDTIQEIKNSIAK
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSEEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFEELVEQLNKK
                     YNMNLDENHYYRDKENYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNAELDLMDGQSDKYFTYLLGNRPLVKIKNVENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(14770..16158)
                     /locus_tag="KRH39_000494"
     CDS             complement(14770..16158)
                     /locus_tag="KRH39_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG8510422.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGVIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            16500..17834
                     /locus_tag="KRH39_000495"
     CDS             16500..17834
                     /locus_tag="KRH39_000495"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBG8510423.1"
                     /translation="MRVVIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFDDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTGAIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEITDVLEEEINRHNVNLHLDEMVVELY
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(17963..18880)
                     /locus_tag="KRH39_000496"
     CDS             complement(17963..18880)
                     /locus_tag="KRH39_000496"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBG8510424.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCVRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            19088..19972
                     /locus_tag="KRH39_000497"
     CDS             19088..19972
                     /locus_tag="KRH39_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBG8510425.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            20185..22026
                     /locus_tag="KRH39_000498"
     CDS             20185..22026
                     /locus_tag="KRH39_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBG8510426.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVSELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEK
                     IKIENKSARLIGKEEALKYKLDSVVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGERITSIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELIRKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFLVSSTLIFLLNFNQKELKDKKSK"
     gene            22331..23038
                     /locus_tag="KRH39_000499"
     CDS             22331..23038
                     /locus_tag="KRH39_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8510427.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFIKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFHYIENKLIIKTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDNSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            23032..24342
                     /locus_tag="KRH39_000500"
     CDS             23032..24342
                     /locus_tag="KRH39_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8510428.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIGYSILSVLIVTLVFILFEYSR
                     LHKLYEYQIKNTQNIAGILIEKYPNDEIDIVKSIYNSDYSKVSIGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMAQE
                     NYLKDTTESEETSYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQINGEINISVEETANYFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRRILEEQGGNIIVSSKIGIGSKFSLFLTTM"
     gene            24459..25142
                     /locus_tag="KRH39_000501"
     CDS             24459..25142
                     /locus_tag="KRH39_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510429.1"
                     /translation="MKILYTENLSKHYGKGESLVKALDNVNLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIWFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKTMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            25135..27522
                     /locus_tag="KRH39_000502"
     CDS             25135..27522
                     /locus_tag="KRH39_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG8510430.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGKMNGVGVEKLEDDSNISLA
                     YMNKDALKLNSFEFVKGKLPIRENEIVLDSGALKALGYKNKDLGEKIKISYDDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISETYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     GQVKEKLNKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     LFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKNK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNLIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAVYYTSGMMIGSILFGGILGYIAV
                     ILLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            27802..28944
                     /locus_tag="KRH39_000503"
     CDS             27802..28944
                     /locus_tag="KRH39_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG8510431.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDKDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTIVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAHSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALKLTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHILNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            28967..29491
                     /locus_tag="KRH39_000504"
     CDS             28967..29491
                     /locus_tag="KRH39_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG8510432.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIV
                     WSNGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFVVPLTSIPGNLMQVLIGLI
                     IALPISKVLRKCIK"
     gene            29591..30409
                     /locus_tag="KRH39_000505"
     CDS             29591..30409
                     /locus_tag="KRH39_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8510433.1"
                     /translation="MLSVTRYSIKNIRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKSV
                     ELLNSFIYANRYSNIKEFCDDTNINIKTLERTCLKYTGYTPKILKRIYRFKMASNQLI
                     YSYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            30487..30798
                     /locus_tag="KRH39_000506"
     CDS             30487..30798
                     /locus_tag="KRH39_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBG8510434.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(30925..32928)
                     /locus_tag="KRH39_000507"
     CDS             complement(30925..32928)
                     /locus_tag="KRH39_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBG8510435.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKILKNYKDF
                     SSFANAHLYYGFHQTNDGWFYREWAPSADSLSLIGDFNNWNRKSHLLRKINSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYRNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEINPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISKLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDSSI"
     gene            complement(33081..33719)
                     /gene="recX"
                     /locus_tag="KRH39_000508"
     CDS             complement(33081..33719)
                     /gene="recX"
                     /locus_tag="KRH39_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBG8510436.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIVERVIDFLKS
                     YNLVNDSVLAKKIVNTNVNLNKCGKNRIKQNLYNKGINRSTIDEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            33995..34684
                     /locus_tag="KRH39_000509"
     CDS             33995..34684
                     /locus_tag="KRH39_000509"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBG8510437.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            34685..35257
                     /gene="yqeK"
                     /locus_tag="KRH39_000510"
     CDS             34685..35257
                     /gene="yqeK"
                     /locus_tag="KRH39_000510"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBG8510438.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            35374..35724
                     /gene="rsfS"
                     /locus_tag="KRH39_000511"
     CDS             35374..35724
                     /gene="rsfS"
                     /locus_tag="KRH39_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBG8510439.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            36078..38498
                     /locus_tag="KRH39_000512"
     CDS             36078..38498
                     /locus_tag="KRH39_000512"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBG8510440.1"
                     /translation="MSVYNFKEVESKWQKIWKDNHQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVKKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWHKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNVVVK"
     gene            38926..39282
                     /locus_tag="KRH39_000513"
     CDS             38926..39282
                     /locus_tag="KRH39_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510441.1"
                     /translation="MGVSTSQLIKIISRKTRMVSSGILIGVADIFKLVLSEDVIKELV
                     IGAVDVEILNRLMEHKNFQCLINLVRIYSQDIVASGDMERNQFIDMVTIFLSNLMRES
                     SKYYTEVRKELTDGIK"
     gene            39381..40049
                     /locus_tag="KRH39_000514"
     CDS             39381..40049
                     /locus_tag="KRH39_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG8510442.1"
                     /translation="METVSNNQSGVEFQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNISLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDINDLITNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            40089..40169
                     /locus_tag="KRH39_000515"
                     /pseudo
     CDS             40089..40169
                     /locus_tag="KRH39_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(40532..40744)
                     /locus_tag="KRH39_000516"
     CDS             complement(40532..40744)
                     /locus_tag="KRH39_000516"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG8510443.1"
                     /translation="MLQKNNLKKIRIHDLRHTSATLLLLGETNMKVVSERLGHTDIKI
                     TMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(41027..41449)
                     /locus_tag="KRH39_000517"
     CDS             complement(41027..41449)
                     /locus_tag="KRH39_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510444.1"
                     /translation="MTFVERCYKYIDMNKNNWSPYTVTNTKSWVKNYIEPFFKNLKLT
                     EVNPSSVQAFVNYNFNNSTFTSAKVRYSFLSSVISEVYRLREIQENSCDFIKLPSKEK
                     YSTNEIYNREEVLLLIEKLLGNNIEIPILLMLLLGLRM"
     gene            complement(41468..41566)
                     /locus_tag="KRH39_000518"
                     /pseudo
     CDS             complement(41468..41566)
                     /locus_tag="KRH39_000518"
                     /inference="COORDINATES: protein motif:HMM:NF026008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            41849..43219
                     /locus_tag="KRH39_000519"
     CDS             41849..43219
                     /locus_tag="KRH39_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBG8510445.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            43578..43736
                     /locus_tag="KRH39_000520"
     CDS             43578..43736
                     /locus_tag="KRH39_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510446.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     repeat_region   44022..44311
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=44022..44050
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaat"
     regulatory      complement(44453..44550)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            44777..45160
                     /locus_tag="KRH39_000521"
     CDS             44777..45160
                     /locus_tag="KRH39_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8510447.1"
                     /translation="MLNKKLPDAELKIMKYIWNTGYKTVISKDVADEIEKTYGWKHTT
                     TITLLARLTKKGFLISQRIGKHVHYTVLVKEREYLKLEGKRIFGGLHNNPLSELISKL
                     HDGEEITEEKIIEIGNWIKSWKDED"
     gene            complement(45338..46333)
                     /locus_tag="KRH39_000522"
     CDS             complement(45338..46333)
                     /locus_tag="KRH39_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBG8510448.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(46454..48082)
                     /gene="aspD"
                     /locus_tag="KRH39_000523"
     CDS             complement(46454..48082)
                     /gene="aspD"
                     /locus_tag="KRH39_000523"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBG8510449.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKNASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
BASE COUNT        18513 a         5504 c         8122 g        16294 t
ORIGIN      
        1 aattagtaag acatatgtta gtatgcaaaa ttctatgaag caggaaccct gtgactttag
       61 tcatgggagg ttcagtttat ttcaaataat attttacgta atccatctat gttaattgga
      121 ttaatagcag catttggtct tgtactacag aagaagaaag catctgatgt tataaagggg
      181 tcactacttg cagctttcgg aattataatt atggagactg gaacaggtat gttagttagt
      241 tcaatagcac caattaatgg agctttccaa agtataagtg gtggtgcagt aacaaaaggt
      301 ttaagtgatg ttacttttac tgcaagttat ggtggaatta taggtttagc aatgtttgcg
      361 ggtttagcta ttcacctaat gatagcaaga tttactccta ttaagacaat cttcttaaca
      421 ggtcacatgc tttattggtt cccattcata tttgtagctt caggagttga agctggatta
      481 aatggtggag tattaatagc ttttgcagca gtattatcag cactctattg gtcaataatg
      541 ccttggttat taagaaaata tgtgtttgat gttataggag atgattcttt tacattaggt
      601 catccatctg gttgtttagc acttatatca ggatttgttg ctaaattagt aggaaataaa
      661 gaaaaatcta cagaagatat aaacatacct caaagtttat cattttttag agaaatatct
      721 ataagtggtg gaatggcagt gtttttaatg tttatagttg ttgggatagt aataccaggt
      781 gcgtttaaag atagtaatga gtttattgga acgtatgcaa taaagcaagg tctttctttt
      841 ggagcaggtc ttataataat gcttcatggg gtaagaatgc taataaatca aataatgcca
      901 gcatttcaag gtatatctga aaaagttgtg ccaaatgcac tacctgcttt tgactgtcca
      961 atattattta attataaacc aaatgctgta ttaataggat ttttagttgc tatgctaaca
     1021 tctacagcta tgattttaat atgtaactac tttaatgtat ttaatttgct acttatacca
     1081 cttgttataa cttcattctt tgagtgtgga gcagcagcag taataggaga atcacaaggt
     1141 ggtatacgtg gagctataat aggtacagct atagctgcaa tatttatggt agttttagta
     1201 ggaatatctg ctgtaattta ctcaggaact attcaaaatt ggatactaat atttggaggt
     1261 aatgaccttt cattatttgg tataatcagt aggtttatag gttcactatt tggaggaata
     1321 ttatagatat gaaatttaaa tacatagatg agataagaag attaattgat ataatagaaa
     1381 acgaagaaaa agtcaatatg gaaaaagcag tacaaatctt tgttgaagca atagaaagta
     1441 aaaatgctat attttcattt ggagcttcac atgcaggaat attaacagaa gaattattct
     1501 atagagcagg tggctgtgtt cttataaatc cagtatttgc aaaatctcta atgttagata
     1561 cagcgcctat aactcacaca agtaagatgg aaagattaga aggatatggt acagctctag
     1621 ctgataaaac tccaattaaa aaaggagatg tagttatagt tcattcagta tctggtagaa
     1681 atccagtaag tatagagttt gctatgaaag ctaaggaaaa taaggcaata gtaatatgta
     1741 taactaattt aagttactca aaaagtgtat cttcaagaca ttcttcagga aaaaaacttt
     1801 atgaagtatg cgatttagtg atagataatc atggtcaaaa gggagatgcc tgtgtagaga
     1861 tagatggatt aaaccaaaaa gttggtccaa cttctactgt aatagcaact actgtgatga
     1921 actctattat agctcaagct actcaggaat tagttaataa agggctaaag aatcctccga
     1981 ttttctatag tgccaatatt gatggtggag atgagttaaa taagaagatt tttgatgaat
     2041 ataaaagtgt aattcattat gaatattaag gttaaaaaaa tatgttaatg aactttgaag
     2101 tttatcaaca aagaatatta ataatatata ataaatgcta tcatttaaaa gctgtaggta
     2161 aaaatctaca gctttttatt attgtagatt aaaaaggtgc gtaattaaat tttaaattgg
     2221 agaaaataaa ttggaaggta tcaattataa atattagaaa cgttgtcata gaaaaaaact
     2281 atattaaaaa aaagtacata gaaaaaactg attagacaat aaatacatgc tttgataata
     2341 ttttaaatgg agtgaaaatg tgtagaatta taatgaaaaa agaaaataaa aataaaaaaa
     2401 gaaaataaaa tggagggaat aaaagtgggt atgttaaaaa aattatgttc actagctatg
     2461 gtagcaatta tgactacaac acttataaca gggtgttcaa gtggaaatgg aaaagattca
     2521 aaaaaggatg gcgaaaaaga aaaactagtt ctttggatgc caccagtaga aaataatatg
     2581 aaggaagttt gggaccctat tttagataaa tttgaggaaa aaaataactg tgaagtagat
     2641 ttacagataa ttccttggga taattattct gaaaaatttg caacagctat aagtgctggc
     2701 gaaggtccag atgtaggata tatgtatgca gaaatgtatc cacagtttat agaaagtgga
     2761 gcagtagaag atttgactaa ttatgcaact aaagaggata aagagcaatc tatttatata
     2821 aaaacatctg aaatgatggg tggaatgtat ggtatgccat tccaagcagc aaatccaggg
     2881 gtattatact ataacaagga tattttagag aaattaggag aaaaacctcc taaaacatgg
     2941 gaagatttta aaagaatttg taaaaaagca acacaagata ctgatggtga tggaaagata
     3001 gaccaatggg gattagctca gggatgggga gctaaaactt ttggtaactt aaactggaac
     3061 tggtatcctt atttatggca agctggtgga gatatattta atgatgattt aaaatcggtt
     3121 aaatttaatg ataagagtgg tttagaagca gctaatttct taaaagaatt acaagcatat
     3181 gtgcctgaag attctttatc taaagatagt aatgaaatga tagaaagcgt atttggacca
     3241 gggaaagcag catttacaat aatgttatct tctgctgcaa caagtgtatt tgatgaatca
     3301 ttcccggatt taaattgggg atttgtaact ggactagaag ataagaaagc agcaactttt
     3361 ggagcagtag accatttatc tttaatgtca tctgctaaaa ataaagaact tgcatacaag
     3421 ttaatacaac atatgttaag tgttgaatca atgacagaat ttcataaagc aataccaaga
     3481 gcaccaataa caaaaggtga accatatcaa ggtgatgaaa gattcaaaga aatggtggaa
     3541 aatgacaaaa atgtatatag accattagta gtaggacctc atggagttga aatatatgaa
     3601 tatttatgga aagaacttca aactatgatt tcaggagata aaactccaaa acaagcgtta
     3661 gatgatgctg caaaatattc aaatgattta ttagcacaat aaaagtttaa agctgtaata
     3721 tgttgtaaag gactttacag attaaaatta gcattgtatt tataaataca atgctaatat
     3781 ttatattgga gaggataaaa tggaaaactt agttagagag tttgaagaaa attcaaaaag
     3841 aaaaaaaaca aaattaaata ttaaaccata tatgtatata ttgccattgg gaataatttt
     3901 agtggcgttt tatgtgatac caataataat gtcaatatac tttagtttta caaagtataa
     3961 tattattagt ccagctactt ttataggttt agaaaattat aaaaaattat ttacagatga
     4021 aacattaaaa gtatctatta taaatactat taaatttact gttgttgtag taccttgcca
     4081 aactatatta tcattgattt tggctgtttg gattacagga aaaggaaata gtaaaatagc
     4141 ttcatttgct aaaggtgcta tatttatacc agtattatca tctatggttt tgataggtat
     4201 ggtatggagg gctttattga atggagaagg ttctattata tatcaggtat taggtgcctt
     4261 tggaatagaa tcatctaaac ttttaggaga tagtaagaca gcattaccta cacttatgtt
     4321 tatatctatg tggaaaaata tagggtattt catggtaatt tatatttcag ctataatgaa
     4381 tttaccaaaa cactgctatg aagtagcgaa ggttgatggg gctacaaaac ttcaagaatt
     4441 tataaaaata actgtacctt tattaaaacc aactacaata atggttgtat ttttagggtc
     4501 gatatggtca ttacaagtat ttgacttaat ttatactgtg acaggtggag gtccaggtat
     4561 atctactatg agtatagtta tgcatgcatt taacttaaac tttaaaaact ttaattctgg
     4621 atatgcaatg actgtagcaa acgtattatt tttgttaata gcagtagttt cgattttaca
     4681 aaataagctt gtaagacgag acaattcaga tttttagaaa ggtaggtata atatgaaaac
     4741 agcaagaaga ttattaagtg atggtttact tttatttata gcgtgtgcaa gtttggtacc
     4801 ttttatatac atgttgataa tatcattgaa gataacatat aactcatata gtttggatat
     4861 atctttttca acagtaaccc ttcaaaacta tatagatatt tttactaaaa aaggatttgc
     4921 acaatatttt tttaatacag caatagtatc tttttcaggt gtattattaa atttagtatt
     4981 tagtacatta gcaggatatt cttttgcgaa gatggatttt aaaggtagtg ataagttatt
     5041 tttatttatg ataatgactt taattatacc ttcccaagtt acaatgatac cactatatat
     5101 aataatgaaa cacttaggat ggataaatag ttatcttgca cttattatgc caataccaac
     5161 tgcatttggc gtatttataa tgagacaagc tattttagga gtaccaaaag agttattaga
     5221 atcagcaaag atagatggtt gctcggattt tagaatttta atacaaatag tgttgccttt
     5281 aattaaacca gcattaatta cactagcaat atttactttt atgggagcat ggaacgagtt
     5341 tatgtggcct cttatagcta caactaaaga tgcaatgaga acattaacag ttggattatc
     5401 tactcttaag acattccaga taactaatta tggacagatg atggcagggg ctacaataac
     5461 atttttacct ccatttattt tctatttgat tttacagagt aaatttgttg aaggtgtgtc
     5521 tttgtctggt ataaaaggtt aaatagtcta atttaataat atataaagaa acttatataa
     5581 atatggagga atttaaacta tggaaatttc atatgtactg aaagattgca cattcaaaca
     5641 caataaatac tcagttgtag aagctgaaac ttggaaagat aaggatttgg atattgtagt
     5701 agctaaaggt gaaaaatttg catttcaaat tatgttaaaa gcaacagaag aatttaattg
     5761 tacaattgat aaaagtagtg ctatatcatg gaaaggcctg ggaaatagag taagattagc
     5821 attaaatgta cctaatagtt tagaaaataa ttttagtata aatatactag gatatgttca
     5881 agatgataca aaagcatttg tgaatgatgc tattttaagg gataaagata tgttagtaga
     5941 gcaatacttg cctcaaacat tttgggtcga aggtcaagtt ccggaagatt ttgaagaaaa
     6001 taactttaat ataggtattg atatatttaa aagctttgga tatgaagatg aagaaaaagc
     6061 ttgtacaatt gacgttcctg tagaagtaaa aaatgttgta ttaaagcctt tagatgaaag
     6121 taagttcttt ttagatttat ggcaacatcc aagctgtcta gctagaatgt ataaagttga
     6181 gctatggtct gatatacatt ttgaaataat cgataactat ctaaaagaat tagcttcttt
     6241 gggagaaaaa gttgcaacaa ttatagtttc agattatcca tgggcagggc aaagttgtta
     6301 taaagtttat aaaaatccat caaacttata tgaatataac atggtaagtg tatcaaaagg
     6361 attggatgga aagattaaat gtaactttga gagtatggat aggtatataa gcatagctga
     6421 taagtataaa atggctaagg agatagattt atttggtcta ttaggtaatt ggtgtgctgg
     6481 tgagtttgga aatcctgtag aaggttataa ggaccctata agagtaagat attttgatga
     6541 aaaggataaa gtatttaaat ttataaacaa tacaaatgat ttaaaagaat atataggatt
     6601 agtattaaat catttaatag agtgtggatt atgggacaga gtaaggatta tagctgatga
     6661 accaaataat ccagaagtag tcaaagagtg tatagaattt ataaattcta ctgtaagtac
     6721 tcatcaagtt aaatataaat cagcaactca cgaccaaaac tttttagaca gagctaaaga
     6781 tgaaattgat gatatgtcaa taaatttaaa acttactata caaaattata aagatataga
     6841 aagtcttaag aaaaaaatca atgacaaggg tgggatattg acttggtttg tatgctgttt
     6901 tccagagaaa ccaaatagtt ttttaagttc tccttttgta gaaaatagaa taataggatg
     6961 gtatacttat tactttggtc ttgatggatt tttaagatgg gactataatt tatggacaga
     7021 agacccttgg aaagattcaa gttacaaatt ccctatttgg aaagctggag atatgttctt
     7081 tgtttatcct ggaaaagatt taaagccagt tagaagtgtt agaatggaga atttaagatt
     7141 tggaatacag gactttgagt tatttacaat gttagaaaaa gaaaagggaa gagagtatat
     7201 agaagttgag cttatgcaag aattattaaa taaaaaagaa aatgctgaaa ttaaaggttt
     7261 tggtgacata gaactaggat attcacttga taattgggga tatgataaag ttaaaaaata
     7321 tgtattagag ttgttagaca gagtataaaa aatataagag tgtctcaaaa tgaacttttt
     7381 agttcatgag gcactctttt tgtatgaaat caatatagtt tcattgtttc aacaatgaaa
     7441 aataggatta tttctattct aagacagccc tttagttttt agttgtattc aatgttttaa
     7501 aactaactat aaacgctata aagtttataa atgacaataa ttatatataa catgatgtac
     7561 tttaatttgc tataataggg attgaataat attatatgga agataacatt gtttgggggg
     7621 attaatataa aaagaatagg tattattata atgttttttt taagtatatc ttttattgga
     7681 ttttatcttg taataagaga taacagtgat acagttaaga tgcaacgagg gataaatata
     7741 ggtaatgcac tagaatcacc taaggatttt ccgtgggatg taaaaatgtc aaataagttt
     7801 tttgatgata taaaagatgc aggttttgac acagtacgta taccagtaag gttttctgat
     7861 tatacgtcag attctgataa ttttaaaatt gatgaagaat tttttaaaaa gattgataaa
     7921 tatgtagatt atgcattgga taaagactta atagttgtgt tggatttaca ccattttgaa
     7981 gaaattatga aagaaccaag agttcataaa gaaaagtttt taaaaatatg gcaacagata
     8041 gctaataggt atcaaaaata tgataaaaaa ttggtatttg aattattaaa tgaacctaaa
     8101 gaaaatttat attcacagtt actaaacgaa tacattgaag aagcaataaa gataattaga
     8161 aaaactaacc ctaaaagaac tattatagta ggaccatata atttctatca aattgattat
     8221 ttgaatgaat taaatattcc taaagattct aatatagtag tttcctttca ctattacgag
     8281 ccaaatgatt ttgcatttca aggaaatatc tatcataagg gatttgagca cttaagcaat
     8341 attacttggg aaggaacaaa tgaacaaatg gattatttaa aaaaaaggtt tgatactgtg
     8401 gaaaattggg caaataaaaa taaggtaaaa atcttcttgg gtgaatttgg agtaacaaaa
     8461 gaagcaccag aaacttcaag gagagcttgg gtaaaagctg taagagaaga agctgaaaaa
     8521 cgaaactttt catgggctta ttgggagctt gcttctggat ttggtatata caatcaaatt
     8581 gaaggaactt gggatagaga tatattaagt gcattaatag aaaagaggtg attttatgaa
     8641 gaaaaattca aaaaagatac ttataacttt aactattatt actaatataa tatatatatt
     8701 atggagaatt ttttatacag tacctaaaga agaaggtatg tttgccttaa tttgtgccat
     8761 aatacttctt tttgtagaga taatgggcat gatggaaatg tttgtacatt attatggtat
     8821 gtcgaatatt gagtatcctg aaaaaccaat cataagtgaa gaactttatc cacatgtaga
     8881 tgtattcatt gcaacatata atgaatctgt tgatttggta agaaaaactg taaatggatg
     8941 tattcatatg cagtatccag ataagaaaaa ggtacatata tacatatgtg atgatggaaa
     9001 tcgtgaagaa atgcgtattc tagcagaaaa aatgggtgta aattacataa ctagaactga
     9061 aagagaagga gcaaaagctg ggaatttaaa taatgcaatg caacatacaa actctccttt
     9121 gattgctaca tttgatgcag atatgatacc aatgcatgac tttttaattg ctacagtacc
     9181 atactttttg aaaaatgagc aagccaaaaa agatggagaa aaagaggagt atgaaaaggt
     9241 tggttttgtg caaactccac agagctttta taatccagac ttgttccaat ttaatctgca
     9301 ctctgaggga aggattccaa atgagcaaga ttatttttac agagatattc aattggctag
     9361 aaataggaca aattctgtaa tttatggtgg tagtaataca gttatttcaa gagaagctct
     9421 ggaagaagtt gatggatttt atacttattc tattacagaa gattttgcaa caggtatctt
     9481 aattcaaagt aaggggtata gatgttatgc tattccagaa gtacatgcat caggtttatc
     9541 tcctacagat ttaaaaagtc ttattaagca acgtgaaaga tgggcacgtg gctgtataca
     9601 gactggtaga cgtctaaata ttttgtttag aagaggttta ggtttttggc agaaaataag
     9661 ttatatttct tcaattactt attggtatgc aagtataaaa cgttttgtat atataatggc
     9721 acctatttta ttttctgtgt ttaatgtgat tgtagtaaaa tgcacattac ttcaagtatt
     9781 ggtattttgg ttgccaatgt atattttaag tagtttatct cttaaaatat tttctcaaaa
     9841 tataagaaat actagatgga ccaatatata tgaaactata atgtttcagt ctctaatgcc
     9901 agcagtaata cttgaaacgt ttgcaatatc taaaaataaa ttttcagtaa caaataagag
     9961 taaattagaa gaaaatagaa tgtataagtt tttacaggga ataccttatt ttatttacat
    10021 ggtcttatct atcataggaa ttttaaaaat gtttgtagca atatttaaaa tgagttcaat
    10081 gacatattca gttgtattat tttggttgat tggaaacctt ttcaaccttg ttatggctac
    10141 attgtttata tcaggaagac aacagctaag aaagtctgag cgttatattg ctgaaattga
    10201 ttttaagtta aagcaaaatt cttatgttct ttcttcaaaa acgattgata tatcagaaaa
    10261 tgggtttgcg tttttgctag aaaatccaga atatatttcc ccagaagaag agtttgaggt
    10321 agagtttaga gaaaagtctg gaaatgaaat gtatatagct aatatgaaag caaagattgt
    10381 caatgttgtg gaagtaaatt caaagtggaa atatgcagct tatattacac atattgaaga
    10441 tagtgaaata gataattgga tgtgtattgt tcacgataga atacctactt taccgatgac
    10501 aatatctaat caactaggtt tctttgatga tttgcaaatt aatgtaaaaa aacgtattga
    10561 aaaaactaga acgttatcac gaagaagtcc aagaatcaat atgaattttc agatggatat
    10621 aaaaaataca ggtaaattaa gaatagtaaa ttttaattat caatatgtat tattaaattt
    10681 tgagaataaa aatatttatc ctaaagaaat agaattggaa attaatgaag gtattgtatt
    10741 agaatgcgat ttatgtgagg ggaaaatcga tgaaagaggt atcttatata gagtaaataa
    10801 cattgacagc ataatgcaaa atttcttttt aagaggtgaa atgatggatt ggatattaca
    10861 aaataagaca atttttgttt caaagcctag tgaaaagaag gaaaaaagta tagatgagtt
    10921 tgaacccatg gaatatattt aggagagatg aaatgaagac tttaaaagtg tttttttgtt
    10981 ctgcaaaata tcttattttg gtgtttattt tttttataaa tactactttt ataagttcag
    11041 caacaagtga taaaatttta gatttatcct ttaagaagat agagactgac ttaagttcta
    11101 agattacata tgaagataca ggtgtaaaaa tagaaacgaa cagttctaaa agtgataagg
    11161 aacgttattt atatatttac caaaatataa aagaaaattg gagtatgtat aataattttt
    11221 atattgaaat tcaaaataaa aataaaagtt cccagagaat aaatttaagt gtacaaagta
    11281 agaatatgct tgaatttagg ttaaaagaag gtagtgaaat atttttggaa gataaaaata
    11341 tagtatattc agataaaata aaagaaggat gtatagaagt accaggtgaa tttgaaggca
    11401 aaatttatgt gaactttaat agccttataa atgagaaaag taatgttgtg ctggactcaa
    11461 atatgctttc aaacattgct agttggggaa ttacgtttat tccatctgat gaagaacata
    11521 atattgttat tataaaaaag atatctttat tgtctaaaga aaaattagga tttttaaata
    11581 atattaaaat aattggcgat gaagaagtac aaataccagt attgggtcaa agtataagtc
    11641 agtatgaggt attaggtctt aaaagtgatt ctaaaattaa gtactctctt atgggaaaac
    11701 aagataatgt gagcatatct caaaacggta aattaacatt aagtaataaa gccaaacctg
    11761 gaaaaattat tttacaagtt aatgttgatg ataagtttaa aattggaaag aaaataacat
    11821 taactgagtc ttggagtgtt aataaaaaag ataaggatgg tgttccatat acattggtat
    11881 ctcctgaaca atcaccaaca gtacaagata ttaaaaaaat aaactttatg aataatatca
    11941 taacgtttgt tcgaatttta tttgtatctt tagtaataat atgctttgga atatatttat
    12001 attggaaaaa atgttcaaaa actaagtaaa gagaggaggt tagattgtgt tattttcaag
    12061 tttaattttt ttattttact ttttacctat aacattggtt ttatattatg tatttaggtt
    12121 taatcgaacg atacagaaca tgatattgct tgctgttagt ttattttttt atgcttgggg
    12181 agagccaaaa tttgttgtta tcatgatagc ttctataata atgaactata tatttggttt
    12241 actagtagat agatataggg aaagtaaaat aaaagtaaaa atctttttag ttttgatgtg
    12301 tgcatacaat ataggtgttt tatttatatt taaataccta gcttttgcgc taagaaatat
    12361 tagtgaactt attaatactg aattgactat acctagtatt gttttaccca taggaatttc
    12421 attttttaca tttcagggaa tgtcttatgt tatagatgta tatcgtagac atggagaagt
    12481 ccaaaagaac cctttttatg tgggattgta tatagcattt ttcccacaat taatagcagg
    12541 tcctatagtt agatatgaga gtgtggcaga acaaattctc aatagaaaag aaacttggaa
    12601 taaattttct attgggactt gtaggtttat aacaggtcta ggtaaaaaag tattaatttc
    12661 aaataacatg gctatagtag cagactatat ttacactatg aatagtcaag gagaaatagc
    12721 tgcatcactt gcatggcttg gttcaatagc ctataccatg caaatattct ttgatttttc
    12781 tgcttattca gatatggcta taggcttagg tcttatgttt ggctttaaat ttgaagagaa
    12841 ctttaattat ccatatatat caaaatcaat tagtgagttt tggagaagat ggcacatttc
    12901 tttgggaatg tggtttaaga gctatatata ctttccgctt ggaggttcta gagtagcaaa
    12961 taaagatatt atgattagaa atatgtttat agtgtggtta tttactggaa tatggcatgg
    13021 agcagaatgg acatttgtaa tttgggggat tttaaacttt gttttcctaa ttatagagcg
    13081 atttataatg tttgaaaaga ttgaaaatca caattttata aaacatattt atgctttatt
    13141 agtagttaat tttgggtggg tattgtttag gtcacctaat ttaaaagaag catataatta
    13201 ttttaaagct atgtttggag gcaatggagc tatatggagt gattatacat atatgttttt
    13261 aaaagaatac tggattttct ttatatttgc atttattttt agtgtgccta ttgcgaagaa
    13321 aatcaataag tttgttgttg aacaagctaa atgtagtgtg atatttaatg tattttatcc
    13381 aatctcagct attttattgt tctttatttc tgtgacatat ttagtaactg gaagctataa
    13441 tccatttatt tattttaatt tttagttgag gaggaaaagt atgaatagat tttttaaagt
    13501 aagattaata actgcaatta tgttcttaat tattttattc gctttctcta tctttaatat
    13561 ttgttattct ggaaaagata cgatacagga aataaaaaat tcaatagcta aaaaaagtag
    13621 tttaaaagat acaatatcat ctattgataa tacagtgaat gacaaagtaa tatacaaaaa
    13681 tacgttcatt gaaacttatg gatatttaca aaaattaatg ggtaaaaatg agttttcaaa
    13741 atttagtgtt gtaaaagata cttctggaaa gatgcattat acatattttg ctaaagctcc
    13801 aaatccagtt ggtgtgatag cagatagggt aagaaagttt tctgaagaaa tggaaaagca
    13861 aaacactaaa ttggtttacc taatgactcc agataagtat ataaaaggag tgacaaaatt
    13921 tccaaaaggg ataccttatt cgtacagcaa tgagacagca gataattttt taaatcaact
    13981 aaaaaagtat aatgtggact atattgattt tagagaaaat attttaaaga gtggtattcc
    14041 aaaagatgat ttatttttta aaacagacca ccactggaaa gtagaaactt ctttttgggc
    14101 gtttgaggaa ttagttgagc aacttaataa aaaatacaat atgaatctag atgaaaatca
    14161 ttattataga gataaagaaa attataattc aattgtatat cctaagtcat ttcttggttc
    14221 tatgggaaga aagactggta tcttatatgg cggtatagat gattttacat taatttatcc
    14281 taaatttaag accaattata cttattatac agattctaaa tctcaaaaat ttgaattaaa
    14341 aggaaggttt gaagagagtt taatattatc atatcccttt aatgcagagc ttgatttaat
    14401 ggatggtcaa tcagataagt attttacata tttattgggg aatagaccac tagttaaaat
    14461 aaaaaatgta gaaaatccag atggattaaa agtgctattt gttaaggatt ctctaattgt
    14521 accgacagca gcattttttt cctctgtttg ttcatctata gatatgatag accctagata
    14581 ttatgatgga gatatactag aatatgcaaa atctcatgac tatgactttg tttttatatc
    14641 tgtatatccc caaaatctta caaaagaatt ttttccgttt tgtgagtaaa tttctaaaga
    14701 aattttctta taaaataaaa aacaaatggc taactggtta aaagttagcc atttgtttta
    14761 agaattattc taagaaatat tttttttact aaacacttct ttatctattt ttccaagtga
    14821 ttttcctaca gtaattgatg ataccatagc accattaaca tttatcatag tacgtcccat
    14881 atctaatata gggtctattg caagtatacc accaactaat ggaaaataag aaccaagacc
    14941 aactccagag ataacaactg aaacggccat tgtagcagtt cctggaagac cagctattcc
    15001 aaacgaacta attgttataa caattaacaa cataacataa aagctcatat ccatgtttac
    15061 tccagccata ttggcaagtg ttacagccat taaagcagga tatatacctg cacaaccatt
    15121 catacccata tttgcaccta aacttgctgt aaagcttgat acaccctcat caactccgtg
    15181 attttgtact agactttcta tcgttacagg caatgttcct aaactagaac gtgaagtaaa
    15241 tgccagaagt aaagcttcag ttgaattttt tatataagta atgggattta gaccatttaa
    15301 agttataaaa attaaatgta tgataaataa tattgcaact gatatataaa gagctaatat
    15361 aaactgtaaa acagatacta ttgaactaat accttgtgaa gttattgtat ttgcaagcaa
    15421 agcaaccaca gcataaggca taaatttaat aacagtcata gacacactta caattatttt
    15481 gtaagatgct tctatgaaat ctataaatgg ttttattgtt tcaaaatgtt ttttacttaa
    15541 tctctttatt gatgtagcta taaacgtagc aaatattatt gtagctacaa tatttccatt
    15601 tgccatagcc attacaggat tagatggtag taatcctctt aaagtatcca caagagagtt
    15661 tatttcttta agttcttgat tttcaactaa agtaggcata ttagctccta cacctaactt
    15721 cattatattt gcaacgataa ttccaacaat tgcagctata gcagtagtag caagaagcat
    15781 acctaaagat ttaaatgtaa gagttccaag attgttatct ttcatattta taattacttt
    15841 tataatggat aagaaaacta aaggtactac taacattttt aataagtcca taaatccaga
    15901 accaaatagg ctataccact tgcttacttc ttgtagccaa gtgacttgag tggtatcatt
    15961 aggaaaacca gcaaaagctt gtattatgac tcccaatata agtcctagta ctgtagcata
    16021 tattgttctc aatgaaaatt ttattttttt cttttcaaat tgtctaacaa taaagaatgt
    16081 acctagaagt aatattatga aaaaaatcgt tttaaaatct gtaatcataa gaaattgtga
    16141 taaaaatgaa ctttccaatg ttttttctcc ttttatttaa aaataaattt ttctaagttt
    16201 aaatgatatc aatatattaa aaaaatgtca atattatatc acaatttact taaatagagt
    16261 aaaacaagtt gattttgaaa aatgtgatta agtatagaaa aatagggtat aataaatgtt
    16321 taaagaattt gaattgtggt aagaaaataa aaaatagctt ttataagatt tgatttcaac
    16381 ttaaaatatg ttaaataaat aactaaaaat tgttatgaaa aatgtttaca tagaatatat
    16441 atcaaatgaa aagcttaaaa taagtgaaga aattaactag acatcaaagg agagataaaa
    16501 tgagagtagt aataattggt ggtgttgcag ctggtatgag tgctgcagca aaattaaaaa
    16561 gaataaaacc tgagtatgaa gttgttgttt atgaaaaaac tgaaatagta tcttttggtg
    16621 cttgtgggtt accatacttt gttggtggat tttttgatga tgctgatgaa ttattagcaa
    16681 gaacaccaga gaaacttaga gaagctggaa ttgatttaaa tatatttaga gaagtcgttg
    16741 aggttgatag tgaatctaag aaaataaaag taaaaaatat aaaaacaggt gcaatatatg
    16801 aagattacta tgataaattg atgatagcaa ctggagcaag aagtattatg cctcctataa
    16861 aaaacataaa attgaagaat gtttctacat taaaaagttt atatgatgga gaatatctaa
    16921 aaaaactttt aagtaatgaa gataataaaa gagtgacaat aataggagct ggttttatag
    16981 ggttagaagc agttgaggca tgtaaaaaat taggtaaaga tgtagatgta attcagttag
    17041 aagatagaat attgccacaa gtatttgata aggagattac ggatgtatta gaagaagaaa
    17101 taaatagaca taatgttaat ttgcacttgg atgaaatggt tgtagaactt tatggagaag
    17161 ataaagtaga aaaagttata acaaacaaag gggaaataga tacagatgta gtaataattg
    17221 ctactggtgt gagacctaat accgagtttt tgtcaaatac taatataaaa atgctaaaaa
    17281 atggagctat agtagttgat gagtatggaa gaacttctgt agaggatata tactctgctg
    17341 gagattgtgc aactataaag aatatagtaa gtaatgaaaa tgtatatgtt cctttagcta
    17401 ctggagctaa taaattaggt agaatagtag gagaaaacct agcaggaaga gaagtatctt
    17461 atcaaggttc actatcttca agttgtataa aaattatgga tatggaggca gcatcaactg
    17521 gaattacaga aagacaagct aaagatttag gcataaatgt caaatctaaa tttatttctg
    17581 attataatca aactcattat tatccaggaa gaaataaaat atatgtaaag cttatttatg
    17641 atgcagatac aaaagttatt ttaggtggac aagtggctgg gtttaaggat gctgtccaaa
    17701 gagcaaatgt attggcagct gctatctttg gaaaaatgac cacaagtcaa cttggaatgt
    17761 tagatttatg ttatgctcca ccatttgcaa gaacatggga tgttttaaat gtagctggaa
    17821 atgtatctaa ataatattta gattatagct aatatagttt acaagcaatt aagggaatga
    17881 atttacctaa aatattctta tcatggtaaa attcattcct aaatttttat tccaaatttt
    17941 tagaaataag gattccatta aattattcat caaaatttaa acgcaagctt aatctatctt
    18001 ctccaagaat agtattttca aaaacattag tagcattttc taaatacata ctaggtatgt
    18061 ctaaagaagg actaaaatga gtcagtaaaa gttgtttgac atttccaagt tttgcaagat
    18121 tagcagcttc tctaaaagtc atatgtttgt ttctaacagc ttttgatata tctaaatcat
    18181 caccatacat ggcttcacat ataaataaat cactatcttt tatgaaatct ggtattgatt
    18241 ctataggtct tgtatcggtt gtaagactta tcttaactcc ctttctcaat tctcctaaga
    18301 ccatatctgg agtgtattgt tttgaatcta aaactatatt ttgaccttct tggagtttgt
    18361 tccataatat tttaggaact tcgtttttag ttgccttatc tatatcaaat ttagggtttc
    18421 ttctaaaatt aaaactgtat cctaaacact ctatagagtg ttctaaactg attgttgaaa
    18481 tttccaaatt tcttaaaact ttattatcta aagaaaaatt gccttgaggg ttttctataa
    18541 tttttaaagt gtatggtaca tattctacta aatttctcat agaacgtaca caatcgacta
    18601 ttccaactgg accaactata gttaaatcac ttgtacgacc actatttcca attgttgata
    18661 aaagtcctaa aagtccaaat atatggtcac cgtgtaaatg ggttatacaa atcaagtcta
    18721 tatctttaaa gccacatttt tttagcttca tagatacctg agtaccttca ccacagtcaa
    18781 ttagtatttt tctaccttta tagtttataa atacagagga tagaaatctg tttggcatag
    18841 gtacatttcc accacatcca agtaaagtta aatctatcat tttttcccct ttctaaaatt
    18901 aacacaaatc tgttgtttgt ttttattatg atatcctatt ataattattt taacattaaa
    18961 tataacaata aatgtgtatt tgagtcaaga aatattgtca aataaagttt ttatataaca
    19021 atgtataaaa atttggagga cgaaaagtga aaattatatt taggagtatt taaaggagga
    19081 aaatttaatg aaagtattaa agaaaattac tattttgtca ttgattacat taactttagt
    19141 tggttgtagt aacggagaag agaaaaaagt ggttgaaggt tctaagaaaa ctcaaaattc
    19201 taaaaaagat gaacagccta taatagtaca taaagatgca aatatattga aagataggta
    19261 tgagccacca aaaggattta aaagagttga tgtagaaaaa ggcagttttg aatcatttct
    19321 taggaacact aagctaaaaa attatggaga gaaagtaaag tattatgatg gaagaactaa
    19381 ggaatcaaaa ggtatttatg atagtgtatt tgatgttgat attggcaata gagatttgca
    19441 tcaatgtgca gatgcaatta tgcttatgag agcagaatac ttgtatcaga ataaactata
    19501 tgatgaaata agttttgatt ttgttgatgg cttcaaggcg aagtattcaa aatgggcaca
    19561 aggatataga atttcagtaa aagatagtgg ttcaagctgg tataaagcaa ctgaatattc
    19621 aacatcttat gagagtttca gaaagtttat ggatatagtg tttgcatatt caggaacgtt
    19681 atctcttgaa aaagaattag agcctgtaaa agttgaggat atgaaaatag gagatgtgtt
    19741 tatagttgga ggaagtccag gacatgcaag tataattatg gatatggcag aaaatgaaaa
    19801 gactggtgaa aaggtattta tgatagccca aagctatatg cctgcacaac aaacgcaact
    19861 tcttattaat cgtaaaaatc caaatttgag tccttggtat agtttagatt ttgaaggtga
    19921 tttgacaata cctcaatgga cttttaaaag agaccagttg aaaagattct aaaataataa
    19981 ttttatataa taaaagaaaa atatagatgt gaaaaaatgt aattagatta aaactaattg
    20041 gatatggaca aaattaaatt gatagataaa tattaaaaat tacactaatt aatcttacta
    20101 tagtttaata tttatctaaa aagttttatg taaaatataa attttatatt aaagctaaaa
    20161 ctaatttgag gaggctaatt tatattgaag attagaaggt atttaatttt ggtaattata
    20221 attctaatta caacaagtca gagttttatt tcttatgggc tagagcgatt tgaagaaata
    20281 actatttttc acactaatga tatacatgga agatacgctg aaggtgatga ccatatacaa
    20341 ataggaaatc tagccacatt gaaaaaagaa actccaaact caattttagt agatgcagga
    20401 gactgtttac atggacttcc aatagtaaat atggatagag ggaaaagtgc aattgaatta
    20461 attaagtgtg ctggatatga ctatattact cctggaaatc atgactttaa ttatggaaaa
    20521 gatagattgt ttgagctatc taaaagtgct aatttaggag agaatacttt aaaattttta
    20581 tcatcaaata tttttgaaaa tgggaaaaaa gtttttgata gtaatgatat aaaagaaata
    20641 gatggaataa aaataggatt ttttggactc tcgacccagg aaacaaagag caaaactgga
    20701 tttaaaaatg tggaagggtt agagtttaaa aatcctatag aaagtgcaac agagcaagta
    20761 agtgaattaa aaggaaaagg tgccaatgta atagttgcaa tatgtcatat tggtacaaac
    20821 agtttaagta agccaaatag tatagatata gcaaatgagg tccaaggaat tgatttgatt
    20881 attgatggac acagccacac taagtttgaa aatggtaaac aagtgggtga tacattgatt
    20941 gtaagtgcag gacaatattt agaaaatatt ggcgagataa aactaacttt ggatactaat
    21001 gatgtagaaa agataaaaat agaaaataaa tcagctagat taattggaaa agaagaagca
    21061 ttaaagtata agcttgactc cgttgtaagt aaaaaggttg atgaaataaa agaacaacaa
    21121 gaaaaaatat tagataaagt tataggtgta actacaaata ctctagatgg ttcatatgaa
    21181 aatgtaagga caaaagaaac taatttaggt aatttaataa gtgacatttt gctagataaa
    21241 actaaggctg atatttcttt atttaatgga gggaatattc gagatacgat agaaaaaggc
    21301 aatataacaa gaagagatat tgtagatgta tttcctttta gcaatactat agttaccaaa
    21361 gagcttacag gagcacagat taaagatgta ttggagcatg gagtaaaact ttatccagaa
    21421 aaaagttcag cctttttgca agtaggagga atttcatatt actttaatcc taaacaaaaa
    21481 gaaggtgaaa gaataacaag tattcaaaaa gaaggaaagc cattagattt aaataaaaag
    21541 tatgtagttg caacgaatga ttatatagcc tcaggtggag atgaatttcc ttgtttttca
    21601 aaagagccag tactaaagga gtttggaaat ttagagagtg cagtcattga atatatagag
    21661 tacaaaaaag aaatatcaaa aaatgtagat ggaagaattg gtattaaatc taaagatgag
    21721 ttaataagaa agaatcatga gagtgatttg atattaagtg aaaatgaaaa aaatatcaat
    21781 gataatgaag agaatctatc taataatata aaaagctctg atagtgataa aaataaaagc
    21841 ttagataaaa caagtgctag agaaagttct aatctaggaa tatctaaaag agaagctaaa
    21901 gaaaaatctc ctaaaacagg agatttaggt ttttcaaata gtataattat atttttagtt
    21961 tcaagtacat taatattttt attgaatttt aatcaaaaag agttaaagga taaaaagtca
    22021 aaataaaaat taatgagcaa aaattagtag atagtaattt aattttagat aatagcaaac
    22081 agcaaaggaa attagttata taaatactaa tttcctttgt tttatacttt aacatgatta
    22141 cttttaaata aaattgtatt ttataaaaaa taattacact aaatggtata gtattatggg
    22201 tatttttagt ttttattcaa gtaaatgaaa tcatattaac aacttctgat atatttacaa
    22261 attgataaaa aaatgatttt attagtatga tataatttag ctagtatgta tgataatgga
    22321 ggatataggg atgaataata tattactttt ggaagatgac aaatctttaa atagaggaat
    22381 tagttttaaa ttaaaaaaag aaggatacaa tgtttttagt gcatttggta tagaagaagc
    22441 aaaaaatatt ttcataaaag aagagatatg cttaattatt tccgatatag gtctacctga
    22501 tggtagtggt tttgactttt gtgaggagat tagaaagaaa agtgatgttt acataataat
    22561 gttgacagct cttgatgaag aggtggatat tgtcacagga tatgatttag gcgcagatga
    22621 ttatatcaca aagcctttta gtttgatggt attaatatca aaagtgaatg cgcttatgaa
    22681 aagggtaaat actgtaaaaa attatactct attggtttgt gatgatttat tttttcacta
    22741 tatagaaaat aaattgatta taaaaacaga tgataaagaa gaagagataa ttttaagtaa
    22801 aacagagact aaactcttaa aatatttgat ggaaaattct atgcagacat tgactaaaga
    22861 gcaactgtta gaatctttat gggataatag tggtaatttt gtagatgata atacgattgc
    22921 agtaaatata aggagactta gacaaaaagt tgaaaaaaat ccatctgaac ctaagtatat
    22981 aaaaacagta agaggggttg gatatatgtg gggagaaagg agtataaaaa aatgctaaaa
    23041 aatatgaacc tcaaagatgg tttaagtgtg aaagatgatt atataatgag aagagttatt
    23101 ggatattcta ttttatctgt actaatagta acccttgtat ttatactttt tgaatatagt
    23161 agattacata aattgtatga atatcaaata aaaaatactc aaaatattgc tggaatatta
    23221 attgaaaaat atcctaatga tgaaatagat atagtaaaat ctatttataa ttctgattat
    23281 agtaaggtgt ctattggaga aaaggcattt aagaaatttg gctatggatt ggaaaataaa
    23341 atgtcaaatg ataaaaattt tagtttatac ttaaaatctt tttttaagga atcatttttt
    23401 atttttttaa tgatgtttat agtagttata ggtataatat attattttat aagatacata
    23461 aataaaagat tgagtaaaat ttattttata gtagataaga tggcacaaga aaactacttg
    23521 aaggacacta ctgagtcaga agagactagt tatctagtag aatataatga gtattttaaa
    23581 gaaggtacat ttagtaagat aaataactgt ctatatgagc ttaatagaag tttaaagata
    23641 aagtttatta aattagaaaa agaaaaagaa agtgtaaaat cattagtaac agatatttca
    23701 catcaattaa aaactccctt agcatcctta aaattataca atacattact tatagaagaa
    23761 gaattagaag aagatgaagc caatgagttc ttattaacca ataaggcttc tataaataaa
    23821 ttagaaaatt taataaattc tttagtgaac atatcaaggt tagaaattag catgataaac
    23881 ataaaaaaag aagataatga tataaaaaat actattctaa atgcagtaaa aagtgttacc
    23941 cctaaggcta agagcaaaag tataacatta aatttaaatg aatttaacag tagaattata
    24001 ccacatgata aaaagtggac tgaggagagt atttttaatg tattagataa tgctgtaaag
    24061 tacacacaga taaatggaga aataaatata tctgtagaag aaactgctaa ttactttaaa
    24121 attataatag aggataatgg cataggaatt gaaaaaaatg agtttaataa tatatttaaa
    24181 cgtttttata gaggttctgc tgatgaaatt gaatctgtag aaggtagtgg ggtaggattg
    24241 tatttaagta gaagaatttt agaagagcaa ggtggaaata ttatagtttc ttcaaaaata
    24301 gggataggga gcaagttttc tttgtttctt acaactatgt aatattaaat gaaaggaaaa
    24361 tgtaagcctc tacatttatt atataagtat aaacagctcc agaaataaaa aattaataaa
    24421 atatggggta atataactta gaatgagagg ataattttat gaaaatacta tatactgaaa
    24481 atttatcaaa gcattatgga aaaggagaaa gcttagtaaa agctttggat aatgttaact
    24541 tagaaataaa tgaaggtgaa tttgttgcaa taataggaaa atcaggttct ggaaaaagta
    24601 cacttttgca tatgattgga ggtcttgata tacctacatc tgggaaggtg tatatagata
    24661 ataaaaatat atttacatta aaagaagaag aacttgctgt gtttagaaga agaaaaatat
    24721 ggtttatatt tcaaagctat aatttaattc catctcttaa tgtatgggaa aatgtagtgt
    24781 tgccaatagg tctagatggt agagaggtag atgaatcttt tataaaagaa cttttaaaat
    24841 cattagggct tgaaaataaa cacgacgttt taccaaatac attatcaggt ggtcaacaac
    24901 aaagggtggc aatagcaaga gcattagcta caagaccagc tataatactt gctgatgaac
    24961 caactggaaa cctagattct aaaacatctg atgaggttat gagtatacta aagactatgt
    25021 ctaaaaaata tagtcaaact ctagttatga taacccatga tgacagtata gcccaaatgg
    25081 ctgacagagt tatatttata gaggatggta gagtatcaaa ggttggtgat aaaaatgatt
    25141 aatactacta agatagcaaa aagtaattta aagcaaaata aatcaaaaag tattctaata
    25201 ataattacta taatactttc aaccttaatg ctttcatcta ttggaattta tattgttgat
    25261 gcaggggcat atcaaaaaga aaatactata aagtattcag gcaattatca aggtatatta
    25321 gcaaatgtag acgaaaaaca agctgatata ttgagtaatc atgcagatgt tgaattaact
    25381 ggtaaaatga atggagtagg tgttgaaaaa ctggaagatg attcaaatat ttctttagca
    25441 tacatgaata aagatgcttt gaaattaaat agttttgaat ttgtcaaagg aaaattgcca
    25501 ataagagaga atgaaattgt attagatagt ggggctttaa aagcacttgg ttataaaaat
    25561 aaagatttag gtgaaaagat aaaaatatct tatgatgact ataaaaatga taaaaaaatt
    25621 gaaaaagagt tcattataag tggaatttta aaaactagcg aaatatctga agcaggtaaa
    25681 tattattatg ctattatatc agaaacatat atgagaaata ctagagatat gtcacaagaa
    25741 gattttaata tatatgtgaa atttaatgat aaaagtaatt tatcaatagg gcaagtaaaa
    25801 gaaaaattaa ataagattgc taatgatata ggattagata ctattaatac tgctgtaaat
    25861 gaaaattata taaatgcttt aaaaccagat atggaaacga ttatgggtgg agtttttgta
    25921 ggattagtaa ttgtattatc tagtatttta gtaatataca atatatttta tatttcaata
    25981 gttacgaaag ttcaagaatt tggaaaactt agagcaattg gtgcaactaa gaaacaaata
    26041 aaaaatatag tgtttaaaga agggtttata ttagccggaa tagcaatacc aataggaata
    26101 attttaggtt atgtacttgc aaatattata ataaaattat ttatggatat agatgtaaaa
    26161 tcatcacgat taccagttat attatcggtt gtggtaataa gctttatatc tgttgtttta
    26221 tcacttttaa agccaatgaa agtagcatca aaagtatcta ttgttgatgc agtaagatat
    26281 acaggaaata aaataagtaa taagaataag agaaaaggat ataaaaatat aaatctaaag
    26341 agattatcac atgccaattt agaaagaaat aaaaaacgta cttacatgac attggcatca
    26401 cttatactta gtggaactat atttataaca gtatcaactg ctttagaaag ttttgatgct
    26461 gagaaaatgg caagagaaca ctttccttat gatatagaag ttagactatc tggttatgaa
    26521 atgaacagtg ataaaaatcc taaaaataat ttgaatattt tacaaatgga taatcctcta
    26581 agtaaagatt tttttaatca aataaagaac atggaaggaa ttaaaagaat agaatctgca
    26641 agaagtgtaa aaattggtat ggaggactat gatgttgaat ttaagtatga ccttttacaa
    26701 agtataaatg aaaatgatgt aaaatcatta aacaagaatc taatagatgg aaaaataaat
    26761 cttgaaagac tgcaaactgg agatgaaata gtaattactc atgttgatac agcaaaagaa
    26821 atgggagtaa aagcaggaga taaaataaga ttaaccttat atgatggaga taaaaagata
    26881 aaaaaagaat ttaaagttca agcaatagct atgggggttc caagttttgg tatagggaaa
    26941 gattttatag atagaacttt gaaatacgat tcaactagta gcttgggaat atatacagaa
    27001 gaagggaagt atcaagaaat caaagatagt atcaagaaga ttgctaaaaa taatggattt
    27061 ttagagactg attttataga tagtagaatt gagtcaaata aggctactat ttcatttata
    27121 aagataatgg gatacacgct aacaggcata ataggtgtga ttggatttat gaatcttgtc
    27181 aacaccatga taactagcat agttactaga aaaaaagaac ttggcatgct tcaagctatt
    27241 ggtcttacta ataaacaatt agtgaaaatg cttaattcag aggcagtgta ttacacttct
    27301 ggaatgatga taggaagtat tttatttgga ggtatattag ggtatattgc tgttatactt
    27361 ctaaaaaaga ctggattatc ttatgcaaca tattctttgc caatagtacc aatcttgttg
    27421 atgattgtgt gtattcttat agctcaattt ataacaactt atttgatagg acgaagtttt
    27481 aataaagaat ctcttattga tagagtaaga tacagtgaat aaaatgcaaa tatttatggt
    27541 tagtttaact agcaaaaaac atataaaaaa tatagttaaa gagattcata attaaatagg
    27601 ttaaagtata gtcacagagc aaacaattta aactgatgtt tgctctgtat tttattgtgt
    27661 aatcaataat atatctaata gactgatatt attcatgttg gaaaatgtat cggtacaatt
    27721 ctaaaaaagt ataaaattgt accatataaa atgaaaattt tgtataataa tcgtatagaa
    27781 aataaatagg agctgatata tatgaataag aagattaatt atgcaccagg accaactgaa
    27841 actagggaaa atgtaagatt agcaagagca gaaaaaacaa caaatccaga tatagatatt
    27901 gattttgttg aattttataa aaagacatgc gagaagtttg gcaatataat tggaacaaaa
    27961 aacgatgttt acatattgag tggtgaagga atattagggc ttgaagcagc atgtgcatca
    28021 cttacagaaa agggagatag agtacttgta attgacaatg gtatatatgg agaaggtttt
    28081 aaagattttg taacaatgta tggtggggag tatgtgcttt tttcaagtga atatactaaa
    28141 agtatagata ttgatgaatt gaaagcattt ttagataagg atagtaattt taaatatgca
    28201 acagtagtgc attgtgatac tccaactggt gtattaaatg atgtaagcaa aatatgtcca
    28261 ttacttaagg aatatggaat attaactata gttgattctg tagcgggtat ggtaggagaa
    28321 cgattaagtg tagatgaatc taaaatagat ataatattag gtggttcaca aaaagctatt
    28381 tctgcaccag caggacttac tatagttgga ataagtcaag atgcaaaaaa ttgtataaaa
    28441 aatagaaaaa ctgatgtaat aggtttttat tgtaatttaa atatatggga aggctattat
    28501 gaaaaaaaat acttccctta tacaatgcca ataagtgaca taatggggct tgataaagca
    28561 cttgataata tattagaaga aggaatagaa aatgtattaa atagacatga aaagatagct
    28621 catagtgtta gaaaatctat tgaggaatat ggtttagaac tatttttaga agaaggatat
    28681 tctaacacag ttacagctat aaaaattcct gaaagtatag gagctttaaa acttactgat
    28741 tatatgctta aaaattataa tacacttgtt gcgacatcac taaatcaata tatggatact
    28801 attttaagaa taggtcatat gggagaaaat gcaaatctaa ataaaataga gcacatacta
    28861 aatgttttgg ataaaagttt aagtgcgtta ggatttaaag gaaatggaaa tttgttaaac
    28921 ttatttaata agtattattt ttaatagata gataggggga tatagtatgg aagttacagc
    28981 taagcaaaaa tttacaacga aagacatggt ggaaacctca ctattaatag cattagtatt
    29041 tattgcaact aaatttataa atataagatt accaatatct ataaatggtg ggcttgtgca
    29101 tttaggaact gcaatgttat tcatatcagc aatagtattt ggaagtaaaa aaggtgcttt
    29161 atcaggagct attggtatgt cactttttga tttaatatct gggtggactt tatgggcacc
    29221 atttaccttt atagttagag gtgttatggg ttatttactt ggaaagattg tatggtcaaa
    29281 tggtaataat gggaataatt ttttaatcaa tgtaatagga atatgtgttt catcaatttg
    29341 gatgttattt ggatattatg taacagaagt tatactttat ggtaattttg tagtacctct
    29401 tacttcaata ccaggtaact taatgcaagt attgataggt cttataatag cgcttcctat
    29461 ttctaaggta ttaagaaaat gtataaaata aatataaagt gaactaaaat ttacgatagc
    29521 ataaattata ctgtataata ttaagatgga gtacataatt aaaaaatttt agtatagagg
    29581 ttagttcaca atgttatcag ttacaagata ttcaatcaaa aatattcgtt taaagccgtg
    29641 ggtgaaattt atttggtatt ttgagacaaa atcaaatata ctgttaaata ataagttact
    29701 tccaacagac agtatagata ttattcttaa tctttctgat gttatggaat ataaaattga
    29761 aaatcaagac tatactgcta gtaatatgca ttttaatgga ataagggata aacatggatt
    29821 tattattcaa catggaaata tacgtgttat agggatttct ttttatccat ttggtctata
    29881 tccattttta aagataccga tttcagagtt taacagacaa attgttgacc tagaagcagt
    29941 atcaaaacta tttgcaaaaa aattagaaga gtcattaaat ccaacaaaag caatagaaaa
    30001 agttgtttta tgtttagaag aagtattatt atccatatta gaagaagatt taatttcaaa
    30061 taaatctgta gagcttttaa attcatttat atatgctaat agatatagca atataaaaga
    30121 attttgtgat gatacaaata taaatattaa aacattggaa agaacctgtt taaaatatac
    30181 agggtataca cctaagattt taaagagaat ttacagattt aaaatggcaa gtaatcaact
    30241 tatttatagt tataaagatg atgaactttt tgattttatt tatgaaaatg agtactatga
    30301 ccaagcacac tttataaaag aatttaaaaa attttcaggg acgtcaccaa ttaaatttat
    30361 tggtgagaat aagacgatta aagaaaatat aaagtacagc tatttataat tatactgtcg
    30421 attttttaca atacctccaa tttatttttt gatatgatat aggaaaataa attggaggtt
    30481 ttacatatga aaattattac agaaatagtt gagtttaatg tggaagaaaa tttgtcaaaa
    30541 gaagagttta tagaaattgt aaatgactta gaattaaaat ttcattctat tcaatccggt
    30601 tttattgata ctgagctttt atatgatgaa aagtctcaaa aatggattat gatacaacat
    30661 tgggaatcta tagaagacct caagagtgct tctagtaaaa tgtttaaaga ttcttcaact
    30721 gagaaattta gaaaagcttt aaagaatcaa actgttaaga tgaatataat accacaaatt
    30781 aaaagttgga aattatagaa ctgaatgtaa aagatagtta aatagtatca aactaaatta
    30841 gccaaataac attttgtatt taaattatta tttggctttt agtgtttatt ttaatatgag
    30901 tgttatatac tagaaaaacc aaccttaaat actactatct aaaagcacta ttgctgtacg
    30961 agaggcaata ggcattttag aagaaggagg agttgagcta aagtatacat cataatctct
    31021 taagttttct ttttttgtgt ttcctccaaa ctctaccata tcagtattta atatcgtttt
    31081 gtagtttgta ttttgaaact ttgaaatatc aactgattgt attagattag ggtggaaatt
    31141 aaatataaat aaatatttgc ccttactgta caccaaaatc tttttatcat tatccatatg
    31201 tataagttca tgactatttt ggataaaaat atcactgtat tttgtaagtt caagcataga
    31261 tttatcaaaa tttaataatt gtttatactt taagttgtta tcttctgaaa ggctccattg
    31321 tcttctggca tacttatagc tccaattgtt gccttctctt ggaaaatcaa tccactcagg
    31381 atgtccaaat tcatttccca taaaatttag atatccttca cctgctagag aaaaagttat
    31441 taatttaatt aatttaataa gagatattgc tctattgata acatggttat ttgagtttat
    31501 ttccatattc cagtacattt ctttatcagc tagccaaaaa ataattgttt tatcgccaac
    31561 taatgcttgg tcatgagatt cacaatatcc tatatttttt tctccaggtc gtctagtagt
    31621 aagttcatac cacattttgc ccaaatccca gtcttcatca gataatttag agatagtttt
    31681 tatccaaaaa tcaggtactc ccattgcaag tctatagtca aatccaattc cgccatcttt
    31741 tatagggata cacataccag gcattccgct catatcttct gcgatactta tcgaattagg
    31801 attgatttct tttattaatt catttgcaaa ttggagatat gttatagctt caatatctgt
    31861 attcatacta aaatattttt catagctatc aaatgaaact ccaagaccat ggttacgata
    31921 taacattgaa gttacaccat caaacctaaa gccatcaaaa tggtactcat ttaaccaaaa
    31981 ttttatattt gaaagcaaaa aatgtattac ttcaggttta ccatagttaa aaagttttgt
    32041 accccaagca ggatgattcc cctttgaacc tgagtggaaa aattgatgtt cacttccatc
    32101 aaactcattt attccttcta aagtattctt tacagcatga gaatgaacta aatctaataa
    32161 aacagcaatc cccattgaat gagcagtatt tatgagattt tttaagtctt caggagtacc
    32221 aaacctagat gagattgcat aaaagtttga aacttggtat ccaaaagagg cataatatgg
    32281 atgttccata atagccataa gctgaatggt attgtatcca gcttttttta tcttaggaag
    32341 tattttttca gtaaattcat tataagttcc aatagattcg ctttcagtag acatacctat
    32401 atgacattca tagattagtg gagaagttat gttttttaag tcaaaatcat tgtcagtcca
    32461 gataaaagga gtttttggtt gccatatttg tccattaaat ccaccaaaat ctttttgaat
    32521 tactcttttt atatataatg gtatacggtc aaatgttttt ccatttgcag taacttgtac
    32581 ctttacctct gatttgtgag gtaagctatt ttggccagga ataaatattt cccaattacc
    32641 agaattaatt tttcttagaa gatgagattt tctattccaa ttattaaagt caccaattag
    32701 tgacaaacta tctgcacttg gagcccattc tctatagaac catccatcat ttgtctgatg
    32761 gaatccatag taaagatgag catttgcaaa agaactaaaa tctttatagt ttttcaatat
    32821 ttttgatttc atatcttcat aatgtttcat tcgctttttt atatcagatt caaatggttg
    32881 taggtataca tcaatatcta aaattttaaa tcttttttga cttttcattg tatttctcct
    32941 ccataataaa tattacatct aaaaaataat atttatctca tactatcttc tctgtataat
    33001 tgatatatat tatataatcc taataaaaag cttaagccac caatgattaa tttcataatt
    33061 tacctcccta gtatttatac ttatatatcg tattcatcaa aatttaaaag tttgtttaaa
    33121 actcttttaa taatatcata atcaaagcct ttataggaaa gatgttgaga aattttttga
    33181 tatattttct ttttatcttc cttctttact ctttcatacc ttttttttgc tagatacata
    33241 gcattttcaa attcagtgtc cttatctaat tcagatactg cttcatcgat tgtacttcta
    33301 ttgatacctt tattatataa attctgctta attctgtttt tgccacattt atttaggtta
    33361 acatttgtgt taacaatttt tttggctaat acactgtcat ttacgagatt ataactttta
    33421 agaaaatcta tgaccctctc aactatattt tcatcaaatt ctgatgatag tttttctttt
    33481 aatttttttt cagattggtc agcatgagat aaaatattta gtgctttatt tttagctttt
    33541 atatacattt catcatctaa aataggtttt aattcttctt catttatttc attccctttt
    33601 tttaaattaa atgtataaac aagttcttta aatatagcaa taaaaaattt atcatccaca
    33661 taaatattaa ctctatcatc atttcttttt tgttgttcta tttttgtaat tatacccata
    33721 agtcctcctt ttttatatgt aattatatta acaaaagcaa ctaataaaat ttaacaaaaa
    33781 acttatattt gtatgttata ttttatcaaa attaagacaa aatagtaaaa agttattttt
    33841 attaaaaagt aaagtagaat catgttaaat aattattttt taaagtgttt tttagcatat
    33901 aaatataatt atatacccaa aaaaacaaat tataagcttt aagaaataaa aaataatggt
    33961 acaatagtat tataacatta aaaggagaca aaatatgaga agtgaaaatc tagttaagat
    34021 ggcagagttg aaaaatactg aaaaactaga gaaatttaat cgccataaag gcaaaatcaa
    34081 aataggtata ttgggaggta cttttgaccc tatacactat gctcatttag caacagcaga
    34141 atttattaga gacaagtatg acattgacaa aataatcttt ataccatcag gaaatccacc
    34201 acataaatta tgtatcacta cagataagta tgatagatat aatatgacac ttctagcaac
    34261 tgaaagcaac gaagattttt tagtttcaaa agtagaaatc gaaagaaaga aaaggactta
    34321 tacaatagat acattaaaat acttaaagaa aaaatacaaa aatgcagaca tttattttat
    34381 aacaggtgca gatgcaattt gtagtgttga agagtggaaa gatgtaaaga aaaactttga
    34441 gttagctact tttatagctg caactagacc aggaattagt ttgttaaggt cacaagagac
    34501 tatagaaaaa ctaactaaga agtataatgc tgacattata acagtttatg ttccatcact
    34561 ggatatatcg tccacatata taagggaaca gctaaatgaa ggcaagtcaa tacgttactt
    34621 agtacccgaa aatgtagaaa attatttata tgaaaataaa ctatatcaat atggagatga
    34681 ttaaatgaac ttagaaaata ttaaccagag actaaatcaa atgttaccag tggggaggct
    34741 tagccattca aaaaatgttg caaaatgtgc tgaaaaatta tgtgagattt atggatgtga
    34801 caaagaaaaa gcttacttag caggaatgat tcatgattgt gcaaagtatt taagtgacaa
    34861 ggaaatagag gattatgtaa ataagtatga aatatattta gacccaatag aggatggaaa
    34921 tcgttccttg tctcatagtg taattggggc gtacatatgc gagtatgagt ttgaagtaga
    34981 agatgaagac attataaatg caatcaaata tcatacaaca gggagagagg acatgtctct
    35041 tttagaaaaa ataatttaca tagctgattt aattgaagaa ggaagaaaat tcccagttgt
    35101 agatacatta agagaattag catatggagg gaagcttgat gaagcacttt taacttcttt
    35161 taataatact ttaatgtttg tcatcaataa aaaagaagag atacatccaa gaacagttat
    35221 ggcaagaaac tatctaatta aagaaaagtt attataaaaa agctatataa aggcaaacat
    35281 attaataaat ttaaatttga ctatgttaca aaatcgtatt aatatgttac aataactaag
    35341 tgtattttaa ttaggaaagt gaggaatttt tatatgacag tggaacaaat gacaaaaata
    35401 gcatacgatg ccatagaaga taaactgggt caagatacag ttataataaa tataggaaaa
    35461 gtttctagtt tgtgtgatta ttttattatt actacagcat cttctcagag acaggtaaaa
    35521 gctattgctg acaatgtgga agatgaatta gcaaaactag gattagagcc aagaggtaaa
    35581 gaaggacaag gaactcaaac ttgggtactc cttgattatg gagacataat ggttcatgta
    35641 tttaatgagg aaaatagagg gttttataat ttagaaaaat tatggaaaga tgcaccttat
    35701 atagatattg acacattagc ataaacatga taatatatgt tattataatt agaaaatttc
    35761 aaaaattata ttaaattgaa tagactagag cagtaaaaga ttatattttt tcagagagtt
    35821 agtggaaggt gagaactaat attatataac tttgaactta ctagattaaa atctattctc
    35881 catggctgga ttaaaagcta tctaagaggg tctatgtata tagtaggcta attagggtgg
    35941 taccgcgaag atttatcctc gtccctaaac gtaagtttag tgacgaggat tttttatttt
    36001 caaagaatta tttggaaatt ataagtaaat aatactaaat aaggtagtta tacacaatta
    36061 aaaataggag gcaaaatatg agcgtatata attttaaaga agtagaatcg aaatggcaaa
    36121 aaatttggaa agataatcat cagtataaaa tggatactgc acaaactgaa aaacctaatt
    36181 attacacatt agaaatgttc ccttatccat ctggaaaaat acacatggga catgttagaa
    36241 attattctat aggtgatgtt gtcgcaagat tcaaaaaaat ggaaggctat aatgtacttc
    36301 atccaatggg atgggattct tttggattac cagctgaaaa tgcagctata aaacatggaa
    36361 tacatccaca taaatggaca atggaaaata tagaagaaat gaaagagcaa ttaaatttat
    36421 taggtattag ttatgattgg gataaagaag ttgcaacttc aactccagaa tactatagat
    36481 ttacacaaga aatatttttg aagtttttag agcatgggct tgcatataag aaaaaatcgt
    36541 atgttaactg gtgtccttct tgtgagactg ttcttgcaaa tgagcaagtt gttcaaggag
    36601 catgtgagag atgtaaagca acagtattaa agaaagactt agaacaatgg tattttaaga
    36661 ctactgaatt tgcagaagaa ttacttaatg acttagatac attagatgga tggccagaaa
    36721 aagttaaaac aatgcaaaaa aattggatag gtaaaagtac aggagcagat ttagtttttg
    36781 atatagatgg aactgataaa tctatgacag tatttacaac tagaccagac acaacttatg
    36841 gagttactta tatggtactt gctccagagc atgaattagt taaagaattg gttgcaggga
    36901 ctgaatatga agcagatgtt gaagcttttg ttaaaaaaat gcataccatg acagaaatag
    36961 aaagaacagc ggctgatgta gaaaaagaag gaatgtttat aggtagatat gttataaatc
    37021 ctttaaatgg caaaaaagtt cctttatgga tagctaatta tgtattagtt gagtatggaa
    37081 caggtgctat aatggcagtt ccagcacatg atgaaagaga tagagatttt gctgaaaaat
    37141 ataatttaga cataattgat gtaataacag aagataacaa aatgataaac tcagaagagt
    37201 ttaatggact agatgcttca gaaggatttg aaggaataat agataagtta gaaaaagaag
    37261 gtagaggaaa gagaacaatt aattatagat taagagactg gttagtttca agacaaagat
    37321 actggggttg tcctatacct gtggtttatt gtgatgaatg tggaatagtt ccagttaaaa
    37381 aggaagattt accagttctt ttaccaacag atgttgaatt tactggaaag ggagaatctc
    37441 cacttactac ttcaaagcaa tttatgtcaa caacttgtcc tcactgtgga aagccagcta
    37501 gaagggaagt tgatactatg gatacattcg ttgattcttc ttggtatttc ttaagatatg
    37561 tggatagcaa taatgaaaat gaaccattta gtaaggaatt agttaataga tggcatccag
    37621 tagaccaata tataggtgga gtagaacatg ctataatgca tttactttat gcaagatggt
    37681 ttgtaaaggc atttaagagt atgggaatgg tagacttcaa tgagccattt aaaaacttac
    37741 ttactcaagg aatggtactt atggatggtt ctaaaatgag taagtctaaa ggaaatacag
    37801 tatctcctat ggatataata gatgagtatg gagcagatac tgcaagactt ttcgtattat
    37861 ttgcagcacc accagaaaga gatttagact ggtcagagca aggtgttgat ggatgcttta
    37921 gattcttaaa tagggtttat agattggttg acgagctagc agacgtggtt aaaaaggatg
    37981 tagagtttgg agaattaaat tctcaagata aagatatgag atatactata cactctacac
    38041 ttaaaaaagt tactgctgat ttaagtgaaa aattcggatt taatactgct atatcagctc
    38101 ttatggaatt aataaatgat atgtataaat ataaagagtt agataatata aacgaagcag
    38161 ttataaaaga aggtgtacaa actatagtaa ctataattgc accatttgca cctcatttag
    38221 gtgaagaatt atggactatg ataggtaaag aaggaagtgt atttgacata gactggcata
    38281 aatatgacga aaaagcttta gtaaaagatg aaatagaagt tgttgtacaa gttaatggaa
    38341 aagttagagg aaaattaact gttaactcaa atatatctaa agatgagatg gaaaaggtag
    38401 ctttagaaga tgaaaaaata aaaggattag ttgaaggtaa aactatagtt aaagttgtag
    38461 ctgtacctaa aaaattagtc aatgtagttg ttaaataatt taaaaatatg aacaaaaata
    38521 taaatttata aaataaatga ggctgtctca aaatagagat aagccttttt tcattgttga
    38581 aattatgaaa atggatttga ttttatacaa aaaaatgatt aaagaactaa aaagttcatt
    38641 cttatacatc ctaattgttg ttttaaacat tattataatt aatgttaaat cgcgttaatg
    38701 tattaagtgg catgtaaatc atttagtttt taaagtattg tttatcgagg aactatccta
    38761 agcttagatg aaaaaaatgt aattattgta tttttgaaat tttttttata taatgaaaac
    38821 aggcaaaaaa agcgcatatc tatggctttc tctgaaacac ccagacaacg aaattcagat
    38881 taatataatg gctaccatat ttctcaaaag gaacttgcaa aaaagatagg cgtttccact
    38941 tcccagttga ttaaaattat cagcagaaaa acaaggatgg tcagcagcgg tattctaata
    39001 ggtgttgccg atatattcaa gttggtattg tctgaggatg ttatcaaaga acttgtgata
    39061 ggtgcagtag atgtggagat tctcaaccgt ctgatggagc ataaaaactt ccaatgcctg
    39121 ataaatctgg tgcggattta ttctcaggat atagttgctt cgggagatat ggagcgtaac
    39181 cagtttattg atatggtgac tatttttttg tctaatttga tgagagaatc ctcaaaatac
    39241 tatacagaag taagaaagga attaactgat ggtataaaat gataaatgca attttaagaa
    39301 ttttgatagt atgattttaa gatttatctg ctattttctt ttttagaaat aattgaaatg
    39361 ttataaggag gtatgtacaa atggagactg tatcaaataa tcaatcaggt gtagaatttc
    39421 aactcagtct gtttgaagct atcaaagatt tattgttaat taatcctatt ttcttgttgg
    39481 gaattatctt gattggtatt ttcttagttg taatattcaa gaaaaatagt gatattccaa
    39541 aggtcaaaac atctatttta tcactggcaa tgtattacta cttatgttta acactatcgc
    39601 atattgttgg tatccctaca ttcagtgagt atataagact gtcacagtta ggggaaacgt
    39661 tctttaatcc aaacataagt cttattcctt ttagtgatgg atttagtttg agttttattt
    39721 tgaatatttt actttttgtc ccattaggtt ttttatgtcc gcttatcagc aaaacttttg
    39781 aacgtttgag aaatacattt ctaattggat ttgggttatc cctctttatc gaaactattc
    39841 agctttttac gttacacaga gcaaccgata taaacgactt aattacgaat gtaattggaa
    39901 caataatagg ttatttctgc ttcaaactga ttgctaaatt gcgaattgta aaactacatt
    39961 cagatgatga ctctatggaa caggattata cagtatgtat tccaattgcc attataatag
    40021 ttgcccttat attgggattt tttagttaag ttccaaatag ttaatttggg aattatggag
    40081 atcattaagt gaataaaatt acttttatat ctttaggtgt aaaaagtatg gagaagtcaa
    40141 ttaaatttta tagagatgga ttaggatttt agattataaa cttacagttt accaaggaga
    40201 tagcaaagtc gtgtgacaaa gggtaaccat ttggaaatcc agacaacata acagatagta
    40261 tgttaccttt tacaggagca tactgtctgt tatcttgtca acatcccatt ttatggtttg
    40321 tgtgtagttt cttgtaaact gaactaaata attaaataga agctattaga attaataata
    40381 acagatagat attaataagc ataaacaaat agtaaaatat gtaaatatag acgtttttaa
    40441 gtgaaactat tataaaataa ttgactgaca atcaactgac aaaacctatt tttttaggtt
    40501 taactgacat ttaactgaca gtcagttttt actatttaaa tagaagttcg cttagattgt
    40561 cagaagcttc tttgtccatt tctttaagta catgagaata tctattcata gttattttta
    40621 tatctgtatg acccaatctt tcagaaacaa ctttcatatt agtttcccct aaaagcagca
    40681 gagtggcact tgtatgtctt aaatcatgta ttctaatctt ttttaaatta tttttttgta
    40741 aaattgtgaa acgtttttct tagtgaagaa ggtagaaacg gatttaaatt ggtgtttaga
    40801 caaactaagt tatttctgtt ttccaggata ccttgcaatt ttaatttgct ttgtcttagt
    40861 ttttcaattt ttcaattaat tcttttggag ctgataaagc tctttttgat tttggtgttt
    40921 ttggctcttt aaaagatatt ttgccattag aatagacaag aatttgatta atatttatca
    40981 gattgttatc taagtcaaca cctgaccacc taagtccagt agcttcttac attcttaaac
    41041 ctagaagtaa cattaaaaga ataggtattt ctatattatt accaagtaac ttttctatta
    41101 gcaataatac ttcttctcta ttgtatattt catttgtaga atatttttct ttagaaggaa
    41161 gttttataaa atcacatgaa ttttcttgta tttctcttaa tctatacact tcacttatta
    41221 cagaacttag aaagctatac cttactttag cactagtaaa ggtagaatta ttaaagttat
    41281 aatttacaaa agcttgtact gaactaggat ttacttctgt gagttttaga ttcttaaaaa
    41341 aaggttctat gtaattttta acccaagatt ttgtatttgt aacagtatat ggagaccaat
    41401 tatttttatt catgtctata tatttataac atctttctac aaatgtcata ccttttagag
    41461 caacaaactt attattattt atagtagact ttatttcaat taaatgtttt tcagcatcct
    41521 ttttcttttc atagcttcca taactttttt gtttcttttt gccagtttct tattccacat
    41581 atacatagaa tttttcccat ctttttctta taaaagctga tttgatattc atatatgtac
    41641 gctctcttta taagttagtt ttataaaaga acagttggat tgctctaatt atgtcattat
    41701 ctttttaatt ctttacagtt acattataac acgtatttgt atacacttaa attaacagga
    41761 agataaatat cgttaaagga ggaaaagaaa atttaatgta gaattatgta agatatatag
    41821 tatttcatat ataaaagggg gagatattgt gaaattaaag aaattattat cactagggtt
    41881 agccgtgtca atgtttgctg ctggtcctat atcagctaat gcacttgata caatttatac
    41941 tattcaagga aaagataaat atgaaacggc tgctcttata gctgatgaac aaaattatac
    42001 tacagctata ttaattaatg cagacagcac aatggctgat ggattaagtg cgagtggact
    42061 tgcaggagca acaaatgcac caattttttt aactaagaaa aataacatac ctaatgcaac
    42121 tcttaaaaga gtagagaaag ctaaaaaggt atacataata ggtggagaaa gttctattga
    42181 taaagcaaca gaaactcttc taaaagataa aggaatagag gctaaaagac ttcaaggtag
    42241 tgatagaatt aagacaagct ataatgttgc aaaagaaata aactcaatta acaaagtaaa
    42301 taaagttata ttgactaatg cttttaaagg agagcctgat gcaatgagtg ctgcacctgt
    42361 agcagttaga gataaagcag ctatagtatt aactgatgga aaaagtgttc catttaatac
    42421 aactggtgta gaaagttatg taataggtgg tacttcatca atgagtgaca aactagttaa
    42481 tgatactaat tcaactagat taggtggagt agatagatat gatactaata aaaaaatagt
    42541 aaataagttc tataatggcg caaaagaatt ttatatagct agtggtactg atttagttta
    42601 tgctttagtt gcttcaccaa tggctaaaaa taatcctatt gtactagttg atattggaag
    42661 taataaagac atacttaaaa atgctgcaaa agtgactggg ataggaaatc taagtgataa
    42721 aattatgcta gagtgtgaaa ataccatcaa taatattagt cttccgtctt caaataaaaa
    42781 tataatatgt ggattaaaga ttgattttga ttcaaagcac ataatttggc aagataaagg
    42841 aagttatctt gaatgttatg atgataacct tgaatattat actgaaaaat ataaagaccc
    42901 aacagttcct ataaatatag aaaatacaag tacacaaaat ttagatgtaa caaaattaaa
    42961 agtttatgga gaagctaatt caacattata tatggggcaa tatgctaaaa taaaaatgga
    43021 ttttaatagt ttcggtgtat taaagccagg agaaaaatgt actatttatt tagatataat
    43081 ttcattagat ccatcaagaa gcatgacatt aacattttct tatgtaggtg ataaagaaaa
    43141 ttataaatat gaacaaggat tggcaagtag aatattattt tataacccag atggagaaga
    43201 tttttttgaa tataaataac tagtgggtat gtaaattaat aagggtttta taagtttaat
    43261 ttttttattt tcgttttata ttaactatgt ggtatgtaaa tgtctcgata ttgctcatat
    43321 gttgagttat tatatttagt ttcatatgaa ctatatggat tttaaaatta aaaatactta
    43381 aaaacattta ctttaatagt aggtgttttt ttgattaaaa gaatattatt ataatgtaaa
    43441 aagtaataaa taggtaaaat atgtaagaat tatatgttat aatattttta gcaagaagat
    43501 gtaatctaca atttatagag tggagttcat gtatcaaaaa attatcctcc cgacgctaag
    43561 aagggaggtg aatatacatg gataattttt tacaaggcat actagcaagt ctatctgcta
    43621 gcttaatagt ttatatagct agcaaactat ttagaaagcg taaaaaacca ctcaaagcgg
    43681 caactaagag tggttgggaa tttgatttaa aaatcagatt ccataaaact aagtaatttc
    43741 taaattatga actccactct acagcaaaat agattgtagt tcttcttgct tttattatat
    43801 cacaaattgg tacagatatt caaaaataat atttttatga tataataaag tcatagaaat
    43861 tttgcagtgt tcgatttttt gaagaaatta aggcttaaca gttgaaatat aaggcattga
    43921 ggacttgtga taagtgttat caattgcact actcatggtt cactgcaaat ttgagagatt
    43981 tgtatatgtg taggcattgg aaatactcaa tttattttgg ggttttatat taactatatg
    44041 gaatgtaaat cctgtagcac cttttattat attttgaacg ctaccacgtt ttatattaac
    44101 tatatggaat gtaaatgggt aggctttccg attttcatgt gtttgatgct cgttttatat
    44161 taactatatg gaatgtaaat atgagagatt tacaacttaa aggttctaat gaattacgtt
    44221 ttatattaac tatatggaat gtaaataatg aactatctaa accaaggtca taatttaatt
    44281 gagttttata ttaactatat ggaatgtaaa ttgtatccaa aaaattacaa taatcatacg
    44341 aactgctaat ataagcagtc aatatatgtg tacatttatc cattttatct ctaaacatat
    44401 aaattattat ccccctaaaa aactttcata aaaacaaaaa gaacaatatc tctttgtcaa
    44461 ctggctgaca taactcataa gatatttata taagtcttag tccctatagc tttgcgtcac
    44521 tagatttctc tagttttgcc gatttagttt tattctataa ctaaataata caatagaatt
    44581 agttattatt caacaagatt gtttgaaaat tgaataaatt tataggtatg aacgtaattt
    44641 cacacttaaa aaacagacta aatacgtaga atatatagaa aaatttactt gtagtttttg
    44701 tgataaaaag ctcctattat taatttaagc tagataagtt tgaggaagtg acataaattg
    44761 aaagaaggaa tatattatgc taaataaaaa attaccagat gcagaattaa aaattatgaa
    44821 atacatatgg aatactggtt ataaaactgt aatatcaaaa gatgttgcag atgaaataga
    44881 aaaaacatat ggttggaagc atacaactac aattacactt ttagctagat taaccaaaaa
    44941 aggtttttta atatcgcaga gaatagggaa acatgtacac tatacagtat tagtaaaaga
    45001 aagagaatat ctaaaattag aaggtaaaag aatttttgga ggtttacata acaatccctt
    45061 gtcagaacta atttcaaaat tgcatgatgg agaagaaata actgaagaaa aaataataga
    45121 aataggaaat tggataaaaa gctggaaaga tgaagattaa taaataaaag gtagctacta
    45181 atataagtgc tacctttttt cttgtggata atattgggga taagttgtgg aaaacttgtg
    45241 gataaaatat ttcgctgaca tttaactgac ataagtatta gattttgatt taaaaatgtt
    45301 gaaatatcaa taaataatta aatagaagtt attagaatta acaatagatt cctttttcaa
    45361 agaagtcttt gatttcatca aggttatcag tttttccaag tgctaagata agttttattc
    45421 tagctttttg acctggcaag tccccaccaa atatacaacc aagatttttt aaatctctac
    45481 cagaaccaaa gtaaccataa ctatcgaata ctctacctga atgacatcta gaaactataa
    45541 ctacaggaag tcctttttct ctagcatatt caactccaga aagcatttga ggaggaatat
    45601 tacctctacc cattgcctca ataactatac ctttataacc actatcaaca gcaaatctta
    45661 taaagtcagc attttctcct acatatgctt taaataaagc aactttagat tctaccttat
    45721 cagtatctat aattgttcta tttacaatat ctctcgtaag gacaagttca ttgcagtcaa
    45781 ttattcccaa tgggccacta gttagagatt taaatgtatt tagagaaaga gtatttgttt
    45841 tagtagcttc agaagctaat aatacttcat tgtttaaaac tacaaggact ccttttccca
    45901 tagcatcttt ggatactgca gtacaaactg atgcagataa attacttgaa ccatcatatc
    45961 caagctcaga actacttctc atagcacctg tgactattac aggtttgatg ttgtcaattg
    46021 taaggtctag aaaataagct gtttcctcta aactatctgt accatgtgtt attacaacgc
    46081 ctgtaatatc ttttcttgct aataaatcat ttacataatt ttttaattcc atcattctat
    46141 aaggtgttat gtgaggtcct ggaatttcat caaaattaaa aacttcaaca tctgcaactt
    46201 tatcaatgtt ggtaaccatt gatagtattt gttcacctga taaagttggt atagctgctc
    46261 cgactttatc atctactgtc atagatatag ttccaccagt aaaaactatg gcaacttttt
    46321 tcttaatact catactattc tctgcctttc ataaataaaa tttataattc cgataggatt
    46381 atatcaaaaa aagtagttaa ggtctacata aattctttta ttcatataat ttagatattt
    46441 cagtgtaata taattatttc ttaatttttt gccattcaac gatataacca tttagtattt
    46501 ctcttatggc ttttcctatc ttagtataag cttcatcata tagatttgct agagatatcc
    46561 taattgacca ttcagggcca tagaatccat ttccactaag taaaactact gaatagtcat
    46621 tagctaattt ataaagaaca tctactggag atgcattttt acttataaat ttaaataagt
    46681 cttctccata gtttaattta gcccattctt caatatcaaa ttgagtgtaa tatgcagcat
    46741 tgtttttgtt ttcattaatt ggaagctcta aagcttcaaa taatagtttt tgacgtctat
    46801 ggcagatact catattaagt tttttgtagg catctacttt gtcaatcaat gcaaaagcag
    46861 agaaaaacgc catttgtact tgctgtggag tagaaagacc agcagtatgg ttgagagcaa
    46921 caagtctgct atcagcaaca actctttcca taaaggaaag actagaaggg ttaagactca
    46981 tatcactgta acgcttatct actgattttt ttaattctcc agttaaatca tttattttct
    47041 tatcaaaaac atttttctta tgaagggcaa atgtaccaag tctccatcct gtaactccaa
    47101 aatattttga ataagaatat actccaacag tgttgtaagg tagttttgac attaaggatg
    47161 aaaaatcttc tacaaaagtt ccatatacat catctgatat tatcattaaa tctttattat
    47221 aattttcaat tacatctatt agattattac aagatgtttc atccatagct atagaagcag
    47281 gattattagg attaacaacg aataaagctt taatagattt gtcacgaagt ttctctaatt
    47341 ctttttttgt atattgccat gtatgagcac ctttttcatc aacttcatta gcatttatat
    47401 ttaccacttt aaagtcgtaa cgaggaaggt taggtatttc aagataaggt gtaaatattg
    47461 gagtcattaa ggctatggta tctccttttt ttagaagttc atttgccatt aatgaatcaa
    47521 atatatagca catagcagct gtagcacctt caacagcaaa aatttctaca tcaccatatt
    47581 tacctgatat atcatatttc atttcttgaa ctaggtaatc gtgtactatt ttttccatat
    47641 gaggtaacat tctatctgga agtggatagt tatcaccaat aattccatca gctagttcat
    47701 gaacaaattc atctccatca aatccaagtt catttatacc aaagtttata atatctctag
    47761 ctaaatatgc tccaggcata tctatatttt catttataaa tttaaaaaat ctttctttaa
    47821 tacccttttt ttgaggcatt ccagccaaat gacctaaatc ccaattagaa cgagtttcag
    47881 taatagcgaa atgaccaaaa gtaaaaaagg cttctctagc agttgaacaa gtccagtttg
    47941 gatttccacg acctgcatca agtaattctc tgttattttc ttttgcagtg tattttgcaa
    48001 tatctattaa tttgtcctta aattcaaaag ggcttatttt tccatatatg tttttgattt
    48061 ctttataagt atgatttttc atgttaaatt cctccttatg taataagttt cacattttta
    48121 taataaaata aacaagttgc aaaaatgatt atactttaat tataataggt ttatagaaaa
    48181 agtaaagtat gaaattaaaa taataactct atttagcctt attaaatata tgttaaaata
    48241 aaaaatacct tttaataaaa aatgattgtt atataaaata aatattcaat ttaattatca
    48301 ccttagtttt tttattgtat tttattatat atgtatagtt ttctaatttt gaataaaaat
    48361 aaagtaaaac tttcttttat attgaagttg tttagtaaga tatgtatttt attaatttat
    48421 taatcttaat aat
//
LOCUS       DAEORZ010000010        47585 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEORZ010000010 DAEORZ010000000
VERSION     DAEORZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47585
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.010"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(151..978)
                     /locus_tag="KRH39_000524"
     CDS             complement(151..978)
                     /locus_tag="KRH39_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG8510450.1"
                     /translation="MKIKDNKAVFHVFIRIFIIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(1026..2462)
                     /locus_tag="KRH39_000525"
     CDS             complement(1026..2462)
                     /locus_tag="KRH39_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBG8510451.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     NLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(2549..3418)
                     /locus_tag="KRH39_000526"
     CDS             complement(2549..3418)
                     /locus_tag="KRH39_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG8510452.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(3576..4229)
                     /gene="phoU"
                     /locus_tag="KRH39_000527"
     CDS             complement(3576..4229)
                     /gene="phoU"
                     /locus_tag="KRH39_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBG8510453.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(4257..5021)
                     /gene="pstB"
                     /locus_tag="KRH39_000528"
     CDS             complement(4257..5021)
                     /gene="pstB"
                     /locus_tag="KRH39_000528"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBG8510454.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(5040..5864)
                     /gene="pstA"
                     /locus_tag="KRH39_000529"
     CDS             complement(5040..5864)
                     /gene="pstA"
                     /locus_tag="KRH39_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBG8510455.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(5866..6783)
                     /gene="pstC"
                     /locus_tag="KRH39_000530"
     CDS             complement(5866..6783)
                     /gene="pstC"
                     /locus_tag="KRH39_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBG8510456.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(7164..7610)
                     /locus_tag="KRH39_000531"
     CDS             complement(7164..7610)
                     /locus_tag="KRH39_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510457.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDFLTYMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVS"
     gene            8268..11315
                     /locus_tag="KRH39_000532"
     CDS             8268..11315
                     /locus_tag="KRH39_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG8510458.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVGI
                     VEQVMNDGSISSTFNEGHLTVEGKDAYCIDINTAFKNGYKTRTDASSRMSADRISDVA
                     LSIEYVKQYTDSHSGISSKHAYLLRQLVVWQRLSVQLDWQCDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNAKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLATLTTDTSGNTEAVEVRATTVYIKELSAPAGFKIDKT
                     VYSLSVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKSNPQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAFHYITRLANAYKVSGDSFYMQDGKNVLPL
                     GTLTVEETKSPSGYLLEGVYMQADGSEEQIKGMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETGDTKAQGGATLKDTTFEIISLNDNAVLVEGKLYKKNEVVKTIY
                     TDIEGIASTSADLLPYGKFRMSEQKPPEGYLTEGAKEIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALLYDTYIIEELRCESNKGFKVIPPFEIVISRN
                     KVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGKDVTIIDTVKLDGLIKGTKYQLK
                     GWQMLKEENEELLINGKRVENDYTFVADEEAMKVEIAYTFNASALGGKNLVTFEELYD
                     LSNPEEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGKKEIEAGKDVTIVDTVK
                     LEGLEVGTKYQLVGWQMLKEENAELIINDKRIENDYIFTADSETMEAKIEFTFDASTL
                     GGKQLVTFEELYDLSNPDKPIKVTEHNDIEDDGQTVTIKEVPETPTPEEPEKPTTPDT
                     PTKTDSPKTGDNTNILAFAIMMFVSAGGLAGTYFFKRRKMKKS"
     gene            11540..11863
                     /locus_tag="KRH39_000533"
     CDS             11540..11863
                     /locus_tag="KRH39_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG8510459.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQRINGRYTPVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            11878..12291
                     /locus_tag="KRH39_000534"
     CDS             11878..12291
                     /locus_tag="KRH39_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG8510460.1"
                     /translation="MRLANGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGADVDWYIKAD
                     DIILKNKGNQPAGNPQNNNPQNLAPQGQQKNKLAN"
     gene            12372..12830
                     /locus_tag="KRH39_000535"
     CDS             12372..12830
                     /locus_tag="KRH39_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG8510461.1"
                     /translation="MKFKNPMLVVTDIDKSVEFYKKVFGLRVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGKNFEVYFEEDDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            12894..13307
                     /locus_tag="KRH39_000536"
     CDS             12894..13307
                     /locus_tag="KRH39_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG8510462.1"
                     /translation="MLDKIPNAEEMTALVGQSLYAIWNKLCALIDEKYDMECLWNKGG
                     KAWTYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTDRNSYSKEVQKIYDET
                     KTYHDGKWLMFEPTDTILFNDFVKLLGIKRKPNRK"
     gene            13362..13964
                     /locus_tag="KRH39_000537"
     CDS             13362..13964
                     /locus_tag="KRH39_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8510463.1"
                     /translation="MDKVVIYIHGKGGNAEESSHYKPLFSDCDVVGLNYTAQYPWEAK
                     EEFPSLFDLICKHYKSVQIVANSIGAYFAMNALSDKQIEKAYFISPIVNMEKLIADMM
                     YWANVTEDELRDKKEIKTTFGETLSWEYLCYVKENPTTWKIPTNILFGEKDNLTSYAT
                     ISEFTKQTNATLTVMRNGEHWFHTEEQMKFLDEWINKSAR"
     gene            14025..14567
                     /locus_tag="KRH39_000538"
     CDS             14025..14567
                     /locus_tag="KRH39_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG8510464.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKRRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            14603..15130
                     /locus_tag="KRH39_000539"
     CDS             14603..15130
                     /locus_tag="KRH39_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HBG8510465.1"
                     /translation="MTNGGIPLETVELRFQYTQSEYIKAERQYLISSKTIHKYDIALV
                     AVFLLLSVVYMLFSSFSVFSILIFGLIIVVTALGSYLYILMPILKFKQTAKYHEEYTL
                     VFSKENIKFKTQSIDSEVKWDIYSALWESHDFYYLIQAPRLYTLIPKRVFKDLNEKQL
                     FEEIAQSRVKTTKHI"
     gene            15241..16635
                     /locus_tag="KRH39_000540"
     CDS             15241..16635
                     /locus_tag="KRH39_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8510466.1"
                     /translation="MRMIWNKGHRIRASDKNLVYRFSLGTLLIVFVVVLLLLNMKQLM
                     RTDWEHISLLENGLTLSPYNFITILIATGVCALVAFWYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVKAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEDMIDCVNAFYEGMVQRSEEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAQARQ
                     DGTATCEAQGDGTD"
     gene            16837..18036
                     /locus_tag="KRH39_000541"
     CDS             16837..18036
                     /locus_tag="KRH39_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG8510467.1"
                     /translation="MNETDFLTALKEKRCAYSVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPDNPMFLLFDYVRIRFPTMDIKHIIKNILKLNIDYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLLAQERSWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIRKCENEECISKFRSFKSYGSGELVKHNETDKYG
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAEQTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTKPKPY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHYQIIKQQTATVEE
                     MVANVKE"
     gene            18044..18178
                     /locus_tag="KRH39_000542"
     CDS             18044..18178
                     /locus_tag="KRH39_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG8510468.1"
                     /translation="MWELVKDFLLVSFGMGIGVVLMCILTVGKEADRCMEEIKEREDN
                     "
     gene            18179..18400
                     /locus_tag="KRH39_000543"
     CDS             18179..18400
                     /locus_tag="KRH39_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510469.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALVAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            18467..18943
                     /locus_tag="KRH39_000544"
     CDS             18467..18943
                     /locus_tag="KRH39_000544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510470.1"
                     /translation="MDNIIEIIEWCNNNTGFATIVLSILTLIVSIIAIFVSLSTARLP
                     YKKRMLVERGTFISSDGIGFHVTATNVGNRQVKISSIGFKINSYVYINKFTLHESQIV
                     LSQGKTTSQYYEIQDFKRALAEMKVSNYTKVYAYVKDTEGTEYKKYFSRISQIMKQ"
     gene            18998..19294
                     /locus_tag="KRH39_000545"
     CDS             18998..19294
                     /locus_tag="KRH39_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG8510471.1"
                     /translation="MNDIFKDMQAKVGCEYLSDLPSYKRKVWHEMKCLNPADYEEKQL
                     EDFSKYVFGMSCQTLKDVMKQQKGREEQCRKQGYWWKRKEQLAKKQHHTGSTCR"
     gene            19213..19716
                     /locus_tag="KRH39_000546"
     CDS             19213..19716
                     /locus_tag="KRH39_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG8510472.1"
                     /translation="MQETRVLVETKRTTGEETTSYWFDLPIEVAEFEEKLGVSTESQD
                     YRIIEKLLPYADEVHEHTSVYQLNELDYMYRQLSSDMQEEYTMLHTVYENLEALYICR
                     NVITLYPDCKSVIDVARQKLMNDPTFKHLSEDCQEYYFDFETYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            19734..20237
                     /locus_tag="KRH39_000547"
     CDS             19734..20237
                     /locus_tag="KRH39_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG8510473.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIEIGEYVSIDELNEMYEMIEELPDYIVDCLDEFIGHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIATSR
                     GMCEIPY"
     gene            20355..20744
                     /locus_tag="KRH39_000548"
     CDS             20355..20744
                     /locus_tag="KRH39_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG8510474.1"
                     /translation="MKKIRSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPAFLTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLQKEIFNETITAVRSVSYVPD"
     gene            20731..23181
                     /locus_tag="KRH39_000549"
     CDS             20731..23181
                     /locus_tag="KRH39_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8510475.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKRLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNLKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEHQLPKKKYKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVIRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSETRGLLHVIDELRCEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGEILSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPIQRNEVE"
     gene            23181..25316
                     /locus_tag="KRH39_000550"
     CDS             23181..25316
                     /locus_tag="KRH39_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG8510476.1"
                     /translation="MKERIQSAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTSSGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALYAVINFVVVFILSASFIACAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SANPEDEDGKTREEVVKTEIENNDNSNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYMAHRARRMERRLAGAVTAGGVAGAVAGSTVSNARTTRNNLAKDNRGNTSSSMGQR
                     AGSKVGAVLDTKNKVKDKANAMKETVKDMPTQTAYAVYSAKEKAKTSVSDFKRGMVQE
                     QQSRQSGRLEKQEQHKKNIADKRMELQKAQEARQARKTDGSATTGATRPHERPATAPT
                     SKTSKPVKINSAKKRPLSSGVSDVKAPQSAQPVHRQNVEKVPVSEKTRQNFKAERTTK
                     AHIVQQPKKITETTEKNRNAVFTKGQKKK"
     gene            25313..26320
                     /locus_tag="KRH39_000551"
     CDS             25313..26320
                     /locus_tag="KRH39_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG8510477.1"
                     /translation="MKLKHIALIGSLFPILFSIVLFFGVLMSADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAKEYGISEYVNVLLAIIQVESGGTAKDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGINDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTANLQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYADLNSSYWKQHLIGAGRVK
                     Q"
     gene            26336..27238
                     /locus_tag="KRH39_000552"
     CDS             26336..27238
                     /locus_tag="KRH39_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG8510478.1"
                     /translation="MFKKNKKPTENVKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISD
                     YLTKELQDLNVDTIRTDIPTSSAVTDVIVWSIEQLGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDADGDMVIIQNPTLAPAIEKSEYEPETLETDASVDADTVNDATAFLE
                     TFFNLYPTATEKELAYYVARNVLEPIGKDYLYSELVNPIFIKDGDNVKVKVAVKFLDN
                     QTKATLVSQYELVLHKDSNWKIVE"
     gene            27341..28156
                     /locus_tag="KRH39_000553"
     CDS             27341..28156
                     /locus_tag="KRH39_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG8510479.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALDCSNDTIIEQLDKKSIEIMDNIQKEQEKLKKIE
                     IAKKEVLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSAFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCAIVNKIGKATGDFKFRFTKPVSMMACTMVYGDFSN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNDKNPENYLTEIQIPLENLL"
     gene            28237..28713
                     /locus_tag="KRH39_000554"
     CDS             28237..28713
                     /locus_tag="KRH39_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG8510480.1"
                     /translation="MTTYKVNSIGTICNNEKGAFIQLEAPYIPALKELDGFSHINVLW
                     WFSDFDNADFRSVLETDQPYKKAPEKMGIFATRSPVRPNPIALSAVEIIDIDYMSGLI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPQVPDWCSHWPHSLEESASFAWESEFNF"
     gene            28727..29278
                     /locus_tag="KRH39_000555"
     CDS             28727..29278
                     /locus_tag="KRH39_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8510481.1"
                     /translation="MSLQDYIFEKPVIETEHLILRTMNKNDIDDLKEWISDISLYEYW
                     GKRPSKSEKNPELLFDKSKKSTKSFHWGIVHKQDSKVIGEIWIYLIENNRMAKVAFRL
                     SKHYQGNGLMSEALKSVIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRE
                     GKMVNTYCDYYLYGILKSDYSTI"
     gene            29545..30456
                     /locus_tag="KRH39_000556"
     CDS             29545..30456
                     /locus_tag="KRH39_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8510482.1"
                     /translation="MYAYENIEMSLNYIEQHLSEKIETEKLAEIACLSTFYYQRLFKR
                     LVKKSVQEYIKLRRLAMVIAELNNSEERILDIALKYGFSDHANFTRAFKEVYHITPDE
                     YRKNVPMLNTFDKPELSSRYIMIDENVPLIVGNIVLEIQRKTLRTKETYFGFEKTVNI
                     AEQIPAGESTGIDVPGQLWREFHAKKLQTENAIFNNDIELGVSHLASPDKGTFLYFAG
                     GMVSSTDVCPKNMVQHTLPAGEYIVCKIEAESFEELVTTALNQANKYLFETWLPNHNL
                     VTKPFMAEKYYKGNAEINYMEIWIIPV"
     gene            30534..30968
                     /locus_tag="KRH39_000557"
     CDS             30534..30968
                     /locus_tag="KRH39_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG8510483.1"
                     /translation="MINIKDINYVPNISEISVYIENSAFDELYQHMNNEYKAVCKIEY
                     SKDVWARGWNVKFKKSGKSLCVVYPKKNYFTVLVVVGNKEKEDVENLLPRLSEYVQEI
                     YRNTREGNGQRWLMIDLKSKNNVYQDVLRLIEIRYAIKCVKP"
     gene            31033..31272
                     /locus_tag="KRH39_000558"
     CDS             31033..31272
                     /locus_tag="KRH39_000558"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510484.1"
                     /translation="MGNEIFRYLRKYFRGLALWKDLSTIPHNQIQSNKALLKIECNTA
                     LKARTNYMFCAGFFHLIGIWIRQRVYYKNKTIRVP"
     gene            31436..32176
                     /locus_tag="KRH39_000559"
                     /pseudo
     CDS             31436..32176
                     /locus_tag="KRH39_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            32127..32738
                     /locus_tag="KRH39_000560"
                     /pseudo
     CDS             32127..32738
                     /locus_tag="KRH39_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            32951..33808
                     /locus_tag="KRH39_000561"
     CDS             32951..33808
                     /locus_tag="KRH39_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8510485.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGVHYLSHGKSPKIKKNYIMEDMLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPAWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            33886..34467
                     /locus_tag="KRH39_000562"
     CDS             33886..34467
                     /locus_tag="KRH39_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8510486.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            34571..35416
                     /locus_tag="KRH39_000563"
     CDS             34571..35416
                     /locus_tag="KRH39_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510487.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGKGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            35413..36096
                     /locus_tag="KRH39_000564"
     CDS             35413..36096
                     /locus_tag="KRH39_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8510488.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKISEYILSKTVALIAISTV
                     IALILGVVSGSNHLLGITVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYMVARHTVKHM
                     WKSLGGVKL"
     gene            36093..36791
                     /locus_tag="KRH39_000565"
     CDS             36093..36791
                     /locus_tag="KRH39_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8510489.1"
                     /translation="MKRILSSTIQVFRQIKSDPMMFAACFTPFVMGALIKLGIPFLER
                     ITDFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            36803..37714
                     /locus_tag="KRH39_000566"
     CDS             36803..37714
                     /locus_tag="KRH39_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG8510490.1"
                     /translation="MEKGRLLQNGKVNRAIQVSKITLFINLSLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDREHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVPSGIALTAAVLSIVIKEWMYWFTRNAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALDIFKESINKMVDHS
                     CDNATETRIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            37767..37907
                     /locus_tag="KRH39_000567"
     CDS             37767..37907
                     /locus_tag="KRH39_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510491.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNRSKDSEDDK
                     MY"
     gene            38164..38790
                     /locus_tag="KRH39_000568"
     CDS             38164..38790
                     /locus_tag="KRH39_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8510492.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKMNMLYQKCLVNGPISYIEDIFNEMIEKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKTQLTTHVKQFVKKYIINEKVK"
     gene            38912..39628
                     /locus_tag="KRH39_000569"
     CDS             38912..39628
                     /locus_tag="KRH39_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG8510493.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            39792..39983
                     /locus_tag="KRH39_000570"
     CDS             39792..39983
                     /locus_tag="KRH39_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBG8510494.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            40007..40237
                     /locus_tag="KRH39_000571"
     CDS             40007..40237
                     /locus_tag="KRH39_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510495.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            40504..40923
                     /locus_tag="KRH39_000572"
     CDS             40504..40923
                     /locus_tag="KRH39_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8510496.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            40926..41180
                     /locus_tag="KRH39_000573"
     CDS             40926..41180
                     /locus_tag="KRH39_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8510497.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALTKMILDFEIDIDIETEEE"
     gene            41555..41758
                     /locus_tag="KRH39_000574"
     CDS             41555..41758
                     /locus_tag="KRH39_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG8510498.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRKLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            41836..43026
                     /locus_tag="KRH39_000575"
     CDS             41836..43026
                     /locus_tag="KRH39_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG8510499.1"
                     /translation="MSKMKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRN
                     TKIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFNFKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISTPKTKNGKRELPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     KLAS"
     gene            complement(43352..44032)
                     /locus_tag="KRH39_000576"
     CDS             complement(43352..44032)
                     /locus_tag="KRH39_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8510500.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(44082..46097)
                     /locus_tag="KRH39_000577"
     CDS             complement(44082..46097)
                     /locus_tag="KRH39_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG8510501.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(46169..46846)
                     /locus_tag="KRH39_000578"
     CDS             complement(46169..46846)
                     /locus_tag="KRH39_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8510502.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(47426..47509)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
BASE COUNT        15884 a         8100 c         8773 g        14828 t
ORIGIN      
        1 taattgcaag attattttat tttaatttta atgctttctc attaatttta tactatatta
       61 tccttacagt atacctctta ttcgtatttt ttatattata tgttttattt tataaataat
      121 ttactttatc ttttactata tttaaattta ttataagttt tcttgccaga aatttttagg
      181 tgttatattt tctgtaattg gcaatattgt atatcctctc tcttttaaga ttcttataat
      241 attatttaaa gcatttaaac tttgctcttt ctcatgaatt aatactacta attcatctct
      301 ttttcttcca taatataata tattacttac tatctgactt gtagaagact tccaatcctg
      361 tgtatccaaa ttccagtccc aaattaaata atcatttgcc ataagtttat tatatgaacc
      421 ttctggcata tatggtttac ttccgtatgg taatcttatt aattttgaac tctgaccagt
      481 tatttcttgt agagtattat tgcatgtatt aaattcccca actgttactt ctgggctttt
      541 atataactta tgtatatcat gacttacact atgaaaacct attgagtttt cttcttccaa
      601 gactctcctt acttcttcct tgtggttttt catattctga ttaagcatga aaaatgtcgc
      661 tttgacatca ttttctttta aaatatccag tagtgcattt gtatattttg atggaccatc
      721 atctattgtt atgtatgcta ctttttcaga agtagcccct ggaccttttg tttcttcttg
      781 agatgctttg tatatgattt ctcctaaagt attactttga gaaagcagtt ccagtgtatt
      841 ttcacctttt gaacttatat tttcaactgg aacattatta attttttcct ccaataatgt
      901 tccagttaaa attaaaacag ctataataaa tatccttata aatacatgaa aaacagcctt
      961 attatcttta attttcatcc cccatatctc ccttttataa aagcataagt ttaagactta
     1021 aaaagttatt ttttatattt attctctttc ttattacttt ttatatctct actaaatatt
     1081 tttttaacct tttcttcttc tttttctcct aaagattcta ctcttttgat tcccaaagaa
     1141 gttaaaaatt gtaatacatt taaattatca aatggagttt ccatattcgt actttcacta
     1201 tattcatcaa cattcttagt accttcttcc acacttaaaa tacgcttcgg acctcctaca
     1261 cagcctccta cacaccccat tccttctata aatgtagctt ctatttcttt atttagaact
     1321 ttctttagac cgtccatgca ctcttttgtt ccttcaaatg ccttttcttc aaattttata
     1381 tttttgtcaa ttcttctaac tgataactta actgctttac ttacacctcc tgttctagca
     1441 taaactctac ctgaaaggga cgcctctact ctattactct catccattat tgctggattt
     1501 aactctaatg catcaaatat ttcttgcaac tctttaaatg taagcacaaa atcaactgca
     1561 tcttttatat cttctaacat tgcttctttc ttttttgcca tacaaggacc tatgaataca
     1621 actttagcat ctggatttaa aatttttaca gctctcccac aagcaatcat aggagaaacg
     1681 gatggtgata cattttccaa tatctgagga aaactatttt gtatcaaact aacccaaata
     1741 ggacagcaac aactagttat aaagcatcct tcttcgtcct ttttcatatg ctcataataa
     1801 tcatatgctt cttttgcagt aagcatatct gctgccaaag caacttctat catatcttca
     1861 aatcctatct tttttaatgc agttcttaat ttaccagggc ttacatcttt cccaaactgt
     1921 cctacaaaag ctggtgcaac tattgcatat acagggctct ttatatcttt taacaatttt
     1981 atcattggta taaattctat tttatctgaa attgcaccca acttacatct tgatacacaa
     2041 tcaccacaag cactacatac atcttcctca ttacaattaa tatgcttact tcttatctta
     2101 catgactcat cttccttatt attttcatca caatatttgc aagcatctga tattacagat
     2161 attattggtc ttttgacttc atctaaattt acagcttcat gtagtgactc ttttaaattt
     2221 ttatctgtat cttctgtcac atccaatccc atagttaccc taattaaatt taccatagta
     2281 tctttatcaa attttccact aaaataatta tttaaattat cctttaaatt ttcatttaaa
     2341 attttattag gtaagttttc aatatcctct atgtcatcat tccacactgc ttttacaagt
     2401 tcgccaaaaa gcttcatttt atatatttct agtattttag aattgtcatt tctagtattc
     2461 ataaaaaata gtacctccat aaattaaact tatatatgtt taatatttac aaagatacta
     2521 ttttttattt attatttaac tactattatt aatatgattt atttagtttt ctcaacaact
     2581 ctatttcaat cattattttc attagatgat agttgaatac agggcttttt tcttcatcta
     2641 tattccagtc tagcttatcc acattatatg ttttcttaag gtatttatat gtatgctctt
     2701 tcaaataaag tttattttca agtaactcta tagactgcat atgaaaatgt atttctctac
     2761 atatccttaa ggcattctca attttttcta tattagtttc actcaaacta taatccaact
     2821 catcaataag ctttgaatat atttcttcta atttttctac ttcttcttca aatttctcta
     2881 catttaaatt ttcttttcta agaattttat cttcaataag caacattgaa agttcttcta
     2941 tctttttaaa ctcatctata acactattta gataatttgg tctagcaaca taataattaa
     3001 caagtactcc aaacaaaaca cccacagatg tatctaacac cctatgtaaa gaataataca
     3061 cagggtcact atttcctaca cctagatgta tagccagaaa ggttacacag gcaactacta
     3121 ctgactttat tttaaacaga ttgcataaat atattgagca cattactcct agaccacata
     3181 aaaatgggtc tccagcttta atgatggcaa atatgaatcc tattatacca cctaaaatag
     3241 taccttccac tctattgatt cccgtcttta aagaaccttt tactgtatct tgaacagaaa
     3301 ttatacatgc taccagagaa taaaacatat tctctactaa atagtcacca agaatcatac
     3361 ataaagtgac actaattcct gtttttatag tcctcatacc catcttttga agtttcaaag
     3421 ttccacctcc taaaaagcac cttatataaa atacgcatcc tcatatttaa tatgttccta
     3481 gtatatcaca attttttaaa tttacccatt aatcatattt ttttatcaat tctttcttaa
     3541 tttagacaat aaaaaaactg ctttaaagca gttttttatc ctatctcaac cattttaccc
     3601 tcaattgcaa aaattatctt ctcacataca tttgttatat ggtctccaat tctttcaagg
     3661 tatcttccta taagtagaag ttttacccct tggtctatgg tatttgagtt ttcatgcatt
     3721 atttttaaac aatcaatttg tacttgttca tacaatccat ctacaatatc atctttagtt
     3781 ccaacatcat aagctatact tgcatcaaat aagattaaag cttctctagt ctgagacatc
     3841 atagacttac attcttttgc catcttagga atatctatta gctcttttat ataagcctct
     3901 ccacctatct tcaacatttc tctagctata tttgctgcat aatctcctat tctttccagc
     3961 tctatagata tattacctaa tgaataaata aatcttaagt cccttgccat tggttgcttt
     4021 aatactatta gctctatact cttatctctt atttcttctc ttaatttatt tatttcttca
     4081 tcatcttcta ctacttttct tgcaccctct aaatccttct ctatcataca agtcacagac
     4141 ttttctattg cttcttcaca tttttccatc ataagcactg tttgttcttt taaagtatca
     4201 atacttatat ctaagctatt attgaccatt gtctaactcc tttatagtta tatattttaa
     4261 ccaaatctac ctgttatata gtcttctgtt cttttatcta caggagtggt aaacatagtt
     4321 tttgtatcgc taaattctat aacctctcca tttaagaaaa aagctgtttc atctgatata
     4381 cgagcagctt gttgcatatt atgtgtaact atgactattg tgtagtcttt ttttaaatcc
     4441 tctataagtt cctctacttt taatgttgaa attgggtcta aagcagatgt aggttcatcc
     4501 ataagaataa cttctggtct catagcaatt gctcttgcta tacatatacg ctgttgttga
     4561 ccaccagaaa gtcctagtgc agatgattta agcctgtctt taacctcatc ccaaatagct
     4621 gcacctttta agctttcttc tacaatttta tctaattgct tcttatctct tagtccatgt
     4681 gtacgaggac catatgccac attatcatat atactcatag gaaatggatt tggcttttga
     4741 aataccattc ctactttagt acgaagattt ataacatcat ctgaggtata tatgtcctct
     4801 ccatcaacag aaatgtttcc tttaattgtt acatcttcga ttaagtcatt cattctattt
     4861 aacgttctta taaaagttga ttttccgcat ccagatggac ctattaaagc agtaacctta
     4921 ttctctttta tatccatatt tattttcttt aatgcttgct tatcaccata aaataaatcc
     4981 aaatctttta cactcatttt tatcttatcg attaattcca ttattcacac ctcttaaatt
     5041 taataattag ctttatttaa tttcttagca acaaatcttg ctagcatgtt tagtgctaat
     5101 acaattacta ttaaaacaat tcctatactg gctgctgttg ctatatctcc actctcttta
     5161 gtcagtaagt atgagtgtac agttaatgtt cttgcactat caaatattcc acctggcatc
     5221 ttggctacag tacctgctgt aagtaatatt gcagctgatt ctccaattat acgaccaact
     5281 gataagatta ctccagataa aattcctggt attgcacttg gaagtactac cttatataaa
     5341 gtttgaaatt ttgtagctcc taatgccaat gatgattctc tataagcttg aggaactgtt
     5401 tttaaagctt cttctgttgt acgaattatt actggtaata ttattattgc aactgtaagt
     5461 gaacctgcaa ctatggaata tcccattttt aatgtaacta caaagaaaat tcctccaaat
     5521 aatccatata ctatagatgg tatccctgat aaactttcag ttgcaaatct tatagctgta
     5581 actgcttttc cttttttagc atattcttgt aaatatatag cagccaatat tcctatagga
     5641 gttgctacaa ctatagaaac tactactgta tataatgttg taattatcat tggaagtata
     5701 ccaccatcac ctgatgctga ataattgcta aataagaaat taaaattcat gttgccaatt
     5761 ccctttataa atataaatcc cactataact actaatgata ctattgtaaa tagtgctgat
     5821 agatatacta atgacttcaa tagattctct ttgaactttc tcatcttaat ttaccgcctt
     5881 atttgaaagt ttatttaaaa ctaagtttaa aattaatata aatgtaaaca atactactcc
     5941 agttgcaaat agcatttctt gatgtgtacc aaatgcgtat cccatttcaa gtgctatatt
     6001 agttgtaagt ggtcttacac tatctgttat tgcagttggc ataactggtg agttgcctgc
     6061 aacaagtatt actgccattg tttcacctaa agctcttcct actcctaaca ctactgcggc
     6121 taatactcca gattttgcag caggtaatac aactttgaat atcgtttcta tttttgatgc
     6181 accaagtgct aatgaacctt ctttgtatgc ttttggtact gctcttattg ctgtttctga
     6241 tacagatatt attgttggta acatcattac tgctaatact attactattg ctaacaaact
     6301 ttgacctttt ggaagattaa atatactttg tatagttgga actatcactg caagaccaaa
     6361 tacaccatat aatactgaag gtatacctgc taaaagttca actgctggtg acattatttt
     6421 tgctaatctt ttaggtgcta cttctgctat aaatacagag gttaatatac caacaggtac
     6481 tcctataaca agtgctccaa gtgttgcaaa tattgatgct actatcatag atgatattcc
     6541 aaatttattg gcagaaggaa cccaatctgc accagttaaa aagtctataa atgaatatcc
     6601 ttcaaatata aatggtctta atcctttata aaatacgaat cctattatca aaagtaaact
     6661 tgcaactgct actaaagcac taagtagaaa aatatttttt gctacttttt caattacgta
     6721 ttttgtctta ttgccattat tgtcattttt ttctaaacct actaatctag attttactat
     6781 cataataccc tcccattaaa atatacttat cgattttcgt tctgtattca aaatttaatt
     6841 ttttataatt cgtcttcatc aaatttccta tacttttatt ctactttttt taagttaaat
     6901 ctatattagg attaagttaa gcaagtgtaa aattatattt actttttaac attaatttta
     6961 cttttaaaac tatatttgac atttacttta caatatactg ttgtttctct atattaaaat
     7021 agcagatata gataataata cagtttatgt ataatttatt acctatatct gctattaaga
     7081 tttgttaact aaatttaact tttatactac atatataatt attgtatcct ctagtttata
     7141 tcactattat ttccatcaaa ttgttacgat acttgatgtt tgcaataggt acgaatagcc
     7201 aaaaatataa aaataggtat ttctataact gagaaaaaca gtataatgtg tggtgtccaa
     7261 aaactcatac ctcctaagtg taagaaatta aatgatgtca tttgatataa taaactaatc
     7321 ttaaaaccaa ttccccctgg cggtaatagg tatccaatcc aactaacacc tattcccata
     7381 ggaattaagc aaatagaaaa agcaatcagc atataagtaa gaaaatcctt acattttgca
     7441 gataaaaata atgttaaact aaccattgca agtattgaaa ttagaccacc aatagctata
     7501 agaatctgtg cttgtagtaa atttatattt actagactaa ttacagaaaa caacatttga
     7561 aatgaggttt tcaagcattc agtaccaaaa gctaaatcag aaattgccaa atggatagaa
     7621 atacatataa caaacattat tataaatata gaaaatagag ctaatatttt agtaaacgct
     7681 agacgaatat gtccatgttt cgtacaacga aggatgctat ctgattgtgt ctgatactca
     7741 ccagaaaaag taggtgcagc aattgcaaca tatataatta ataatataaa aatataaaat
     7801 attatataat cgaaagcatc ttctggattt attaagaaat tcttaatctt attatttaac
     7861 tattttatta tttaatatat attaaaggta aaaaaataat atatagattt agatatacaa
     7921 tttatctatc ttttcaaata taatctataa aataatagtt ctgttacgca tttttttata
     7981 taaaaaacac actaaaaatt tgattatccg ctaaaacgct gtatctatcg gcattatcaa
     8041 ggagctaaac acatcaaaat actactaatt tactacgatt gaatgagcct tgatacgtcc
     8101 tatttcccag atatgcaaag atacggtaca gcacatcagt attgaaacaa gaaaaattga
     8161 atacttcata gactacggaa cgcacaatat cagcggtcct tttctatttc tagccgttcc
     8221 tagtggacgg ctattttact accaaaatga aaggagcatt tagaattatg aaaaagatat
     8281 taaaacgatt gtgtacaggc tttcttgcct ttgcaactgt cgttaccgct ttaccgagtt
     8341 caacggttca tgcgtctaat acacagtatt ggacggaaag taaagagcgt gtcggtatcg
     8401 tagaacaagt aatgaatgac ggttctatca gttctacctt taatgaggga catttaaccg
     8461 ttgagggcaa ggacgcttat tgtattgaca tcaacacagc gtttaaaaac ggctataaga
     8521 ccagaacaga cgcaagctca cgcatgagtg ccgaccggat aagcgacgtt gccttatcta
     8581 ttgaatatgt gaaacagtac acagacagcc acagcggaat aagcagtaaa cacgcctacc
     8641 tattaagaca gcttgttgtc tggcagagat taagtgttca gcttgactgg caatgcgata
     8701 atgtgcgagc ttcctatgat gaaatcccaa aagccgtaca agatgaagta ttcgcaggag
     8761 caaaagcctt tgtgaaagaa aacaaaggac gttatgactg tggcggatat atctattctg
     8821 gcgagggtca agagttaggg caattctggg cgaagttagc tgtcggaaat gccaaacttc
     8881 aaaagacttc caccaatgcc aatattacag acggcaacgg gatttattca atcgctggtg
     8941 caacatacgg tgtgtattct gataaagact gcacgaaaca gcttgccacc cttacgactg
     9001 atactagcgg aaatacagaa gccgttgaag taagagccac tacagtatat atcaaggaac
     9061 tttctgcacc agcaggattt aaaattgata aaacagttta ctctttatct gtggaagctg
     9121 gaaaaacagc gactttaaaa gtatctgata ctccaaaagt aacagatacc ttgattgagc
     9181 tattcaagat tgacatggaa acacagaaat ccaatccgca gggaaacgct tctttagagg
     9241 gagcagagtt tacatggaac ttctacgctg gatattacaa caagaacaat cttccagcac
     9301 agcctacaag aacatgggta acaaagacta tcgctgaaaa agacagcgac ggtgctttcc
     9361 attacattac ccgattagct aacgcttata aagtatctgg ggacagcttc tatatgcagg
     9421 acggtaaaaa cgtacttcca ttaggaacac taactgttga ggaaacaaaa agtccgtcgg
     9481 gttacttgtt agagggtgta tatatgcagg ctgacggtag cgaagaacag ataaaaggaa
     9541 tgtatctgac acagattaca gaggacggcg accttgccgt attatctgga agcaatcaat
     9601 accatgtttc tgataaagtc attcgtggcg gtgtgaaaat ccaaaaacga gatttagaaa
     9661 cgggtgacac gaaagctcaa ggcggtgcga ctttgaaaga taccaccttt gaaatcattt
     9721 ccttaaatga taatgctgtc ttggttgagg gcaagctata caagaaaaat gaagtagtaa
     9781 agacaatcta taccgacatt gagggtatcg cttctacttc tgctgatttg ttaccttacg
     9841 gaaaatttag gatgtctgaa caaaaaccgc cagagggata tttgacagag ggtgcaaaag
     9901 agattgactt tgaaatcaca gaaaatggaa aaatcgtaga tttgactgac gaagcccact
     9961 ctatctataa ccagattaaa cgtggcgata ttgagggcgt aaaaattggt gcaggttcgc
    10021 acaagcgtct tgcagatgtt ccgtttagga tcacaagcaa gacgacgggc gaaagtcatg
    10081 ttgttgtaac tgatgataac ggacaattct ctactgcttc tgattgggct tcccacaagc
    10141 acaataccaa tgcaggaaaa acaagcgagg acggtgtgtg gtttggtact tctgaaccag
    10201 acgacagcaa aggtgctttg ctttatgata cctacatcat tgaagaatta cgttgtgaaa
    10261 gcaacaaagg cttcaaagtt attccaccgt ttgagattgt catttccaga aataaggttg
    10321 tcgttgattt aggtacgctg actgatgaat acgaaaaaga aatcactatc catactactg
    10381 ctaccagcaa ggacggcgaa aagactattc ttgctgggaa agacgtaact atcattgata
    10441 cagtcaaatt agacggtctt atcaaaggga caaaatatca gttgaaaggc tggcagatgt
    10501 tgaaagaaga aaatgaagaa cttcttatca atggtaaacg agtagaaaat gattatactt
    10561 ttgtcgctga tgaggaagct atgaaagtgg aaatcgctta tacattcaat gcgtctgctc
    10621 ttggcggtaa gaaccttgta acctttgagg aattgtacga tttaagcaat ccagaagaac
    10681 ccgtgaaagt tgcggaacac aaggatattg aggacgacgg acagacggtg cttatcacag
    10741 aacgtatcat caaaattcat acgacagcta cggacaagga cggcaagaaa gagattgaag
    10801 ctggaaaaga cgtaactatc gttgatacag tcaagttaga gggcttagaa gtcggtacga
    10861 agtatcagct tgtgggctgg cagatgttaa aagaagaaaa tgcagagctt atcatcaatg
    10921 acaagcgtat tgagaatgac tacatcttta cggctgacag cgaaactatg gaagcaaaga
    10981 tcgagtttac ctttgacgct tccactcttg gcggtaaaca gcttgtaact tttgaggaat
    11041 tatacgattt aagcaatcca gacaaaccta tcaaagttac ggagcataat gacattgaag
    11101 acgacggaca gacagttact atcaaggaag taccagaaac acctacccca gaagaaccag
    11161 agaagccaac tacaccagat acacctacga aaacagacag ccccaaaacg ggcgacaata
    11221 caaatatcct tgcctttgca attatgatgt ttgtttctgc tggtggtctg gctggtacat
    11281 actttttcaa acgccgtaag atgaagaaat cataaggcgg tagctgtatg agaaaagaaa
    11341 aatcacgctc tccgccgtag cttagatagg cagactatac ctattctggc ttgtctgccg
    11401 attacctgca agaacacgga tagtcaaggg tgcgttagca tttatttacc ctttactatc
    11461 tgggggcttg ctggtacttt cctaaatgcc agaatacaca catcaatcaa acttaaagaa
    11521 agaaacgagg taaaacaata tggaattaaa atatatcgtg ccgaatgtgg cacagacttt
    11581 tggaaacttg gaatttgcag gggaaaacaa agtagaccag caacgtatca atgggcgtta
    11641 cactcccgta tcaagaagtt acaatttgta ttcggatatt cagagagccg acgacattat
    11701 cgttgtattg cctgcaaaag ctggcgagaa gcattttaag ccagagcaga aagtaaaact
    11761 gattaaccca cgcattaccg cagagggtta caacgtcaaa ggacgtggat ttacaaacta
    11821 cattttaatg gctgatgata tgttaccagc agaaagcaaa taagggggta tttgattatg
    11881 agattagcaa atggaattgt attagataac gaaaaaactt ttggagtatt gaaattctct
    11941 gcacttcgac atgaggtgcg tgtagaaaat gaggacggaa cgacaagtga tgaaattaag
    12001 cgtcgtactt atgatttgaa atgtagtgtg caggaacgca tgatacaagt gtcaatcccc
    12061 gctgatgttc ccgtaaagga ctttccttat aatgcagaag tggagttaat caatcccgta
    12121 gctgatacgg tggcaaatgc caactttaga ggggctgatg tggactggta catcaaggct
    12181 gacgatatta tcttgaagaa caaaggcaac cagccagcag gaaatccaca gaacaacaat
    12241 ccacagaacc ttgcaccaca aggacaacaa aaaaataaac tggcaaacta gattttcatt
    12301 gtagcaaata taagtgactg ttataatatg ggtatggaaa cagataattt gaacttgcag
    12361 aggtagcgaa aatgaaattt aaaaatccta tgttggtagt gactgatata gataaatcag
    12421 ttgagtttta taaaaaagtt tttggacttc gtgtaattat ggattttgga gcaaataaaa
    12481 ctttaacagg tggcttagcc ttacagacaa gtgaaacata caaagagttt atcggtacaa
    12541 gtgatatttc ttttggcggt aaaaattttg aagtctattt tgaagaagat gattttgata
    12601 aatttgctga taggctgaaa gaatatgata ttgaatatgt ccaccctatt atagaacatt
    12661 catggggaca acgtgtagtc cgcttttatg acccagataa acatattatc gaagttggag
    12721 aaaacatgaa aatagtatgc aagcgtttct taaatagcgg aatgacacca gaacaagttg
    12781 cagaacgcat ggacgtgcct atgaaattta taaacgcctg tatgagataa attcttgttt
    12841 gtagaagtca ataaatacaa agataaataa gaagttataa aggagaaatg agtatgctgg
    12901 ataaaatacc taatgcagaa gaaatgactg ctttagtcgg gcaatcatta tatgctatat
    12961 ggaataaact gtgtgcctta attgatgaaa agtatgacat ggaatgttta tggaacaaag
    13021 gtggtaaagc gtggacttat gagtataaat atcgtcgtgg cggaaaaacg ctttgtgcat
    13081 tgtacgcaag ggaaaactgt gtaggattta tgattatctt agggaaagat gaacgattaa
    13141 aatttgatac agatagaaat agttattcaa aagaagtgca aaaaatttat gatgaaacaa
    13201 aaacctatca tgacggaaaa tggttgatgt ttgagccaac agatacaatc ttatttaatg
    13261 attttgttaa attattggga ataaaaagaa agcccaacag gaaatgatga ttttatactt
    13321 caatttagta tacaaatcgg aagttgagga ggacaactat tatggataaa gtagttattt
    13381 atatacatgg taaaggtgga aatgcagagg agtcttcaca ttacaaacct ctttttagtg
    13441 attgtgatgt ggttggactc aactatactg cacaatatcc atgggaagca aaagaagaat
    13501 ttccatcact ttttgatttg atttgcaaac attacaaatc tgttcaaata gttgcaaata
    13561 gcataggtgc ctatttcgct atgaacgcct tatcagataa gcaaatagaa aaagcatatt
    13621 ttatttcacc tattgtgaat atggaaaaac tgattgcaga tatgatgtat tgggcaaatg
    13681 ttacagaaga tgaacttcga gataaaaaag aaattaaaac aacttttgga gaaacactct
    13741 catgggaata tctctgttat gtaaaagaaa atccaactac atggaaaatt ccaacaaata
    13801 ttttatttgg tgagaaagat aacttgactt cttatgcaac tatatctgaa tttacaaaac
    13861 aaactaatgc aacacttact gttatgagaa acggagagca ttggtttcac acggaagaac
    13921 aaatgaaatt tcttgatgag tggataaaca agtctgccag ataaatccca gtttgtaaaa
    13981 gtcaataaat acaaagataa atttgaagtt aggagactgg tactatgaaa ggatttgaac
    14041 gaaaaaacca attattttct ctttgcggat tgaattgcgg actatgccct atgcttttgg
    14101 gaaattactg tggcggttgt ggcaatggta atcaatcatg cagaattgca aaatgtagtc
    14161 ttgaacatgg acagattgaa tattgctatg aatgcaaaca atatccatgc gaaaaatatc
    14221 aacacattga tgattatgat tctttcatca cgcataaacg gcgaaaagca gatttagaaa
    14281 gagcaaaaaa cataggcatt gagcaatata atcttgaaca gcaggaaaaa acacaaattc
    14341 tttcttactt actttctaac tataatgacg ggcgtagaaa gactttcttt tgtgtggcag
    14401 tcaatttatt agaactttca gaattacaag aagcaatgaa acaaatacaa tccaatgacg
    14461 aattgacctt actaccattt aaggcacaat gcttgtatgt ggtggaggtg tttcagaaga
    14521 ttgctgaaag gagaaatata aaactgaaac ttttcaagaa aaagtgattt tcaaatttga
    14581 aattgtagca aagattattt atatgacgaa tgggggaata cctttggaaa cagtagagtt
    14641 aagatttcaa tatactcaaa gtgaatatat aaaagcagaa aggcaatatt taatttctag
    14701 taaaactata cataaatatg atattgcttt agtcgctgta tttttgttgc tgtcagtcgt
    14761 ttatatgctg ttctcctctt ttagcgtatt tagtatttta atatttggat tgataattgt
    14821 tgtaactgca ttgggaagct atttatatat cctaatgcct atactgaaat tcaaacaaac
    14881 tgcaaaatat catgaggaat atacgctggt attttctaag gaaaacatta aatttaaaac
    14941 acaaagcatt gactcagaag tgaagtggga catttactct gcactatggg aaagccatga
    15001 tttttattac ttaattcaag caccacgatt atatacgtta attcccaaaa gagtttttaa
    15061 agacctaaac gaaaaacagc tatttgaaga aattgcacaa tcaagagtta aaacaacaaa
    15121 gcatatataa tgaacatacc ctacaatgta acaacttcat attcatacac acaaagcggt
    15181 tagtctttta agattgactg cttttttcat acttaaattt aagattggag tgattgattt
    15241 atgcgaatga tttggaataa aggacaccgt atcagagcca gcgataagaa cctcgtctat
    15301 cgcttttcat tgggaacgct tctaattgta ttcgtggtgg ttcttctgct actgaatatg
    15361 aaacagctca tgcgtaccga ttgggagcat atcagcttgt tagagaacgg cttgacgctt
    15421 tccccttaca acttcataac catactgata gcgactggtg tttgtgcatt ggtcgctttt
    15481 tggtattacc gcttctgcta tgacagtttc aagaagctac tgcaccgtca aaagctggca
    15541 agaatgatac tggaaaacaa gtggtatgaa gccgataccg tacaagacag cggttttttt
    15601 actgacctgc aaagcagatc aagggaaaaa atcgtctggt ttccaaaaat ctattatcaa
    15661 atggaaaaag gattgcttca tatccgctgt gaaattacgc tggggaaata tcaagaccag
    15721 ctcttacgat tagaggataa attggaaagt ggcttgtatt gtgaactgac cgacaaaacc
    15781 ttacatgacg gctatatcga atataccctg ctttatgata tgatagcgaa ccgcattacc
    15841 attgacgaag tgaaagcaga aaacggctgt cttagactga tgaaaaatct tgtatgggaa
    15901 tatgacgcac ttcctcacgc tctgattgct ggtggaactg gtggcggtaa aacgtatttt
    15961 ctgctgacgc ttattgaagc tctgctacat acgaatgctg tcctttacat cttagaccca
    16021 aagaacgctg accttgcaga cttagggaca gttatgggaa atgtgtatca caccaaagaa
    16081 gatatgattg attgtgtcaa tgccttttat gagggcatgg tacaaagaag cgaagaaatg
    16141 aagcgacacc cagactataa aacgggcgaa aactacgcct atcttggttt accgccctgc
    16201 tttcttatct ttgatgaata tgtggcgttt tttgaaatgc tggggacaaa agaaagcgtg
    16261 agcctactta gccagctaaa gaaaatcgtt atgttgggac gacaagctgg atatttcctt
    16321 attgttgcct gccagcgtcc agacgcaaaa tatttctcgg acggtatcag agataatttc
    16381 aatttccgtg ttggattggg gcgtatcagc gaattgggtt acggtatgct gttcggttca
    16441 gatgtgaaaa agcagttttt ccagaagcgt atcaaggggc gtggctattg tgatgtggga
    16501 acaagcgtca taagcgaatt ttacacgcct ttagtcccga aaggacatga ttttttgcag
    16561 actatcggct ctcttgcaca agcaaggcag gacgggacgg cgacgtgcga agcgcaaggc
    16621 gacggcacgg actagccgtt gctggtgtgg cgttagccac gccagcagaa acaagcccct
    16681 cattatctaa cagaggggta caattacggc ggaaacttcc acaaaaacct ttgcaagacc
    16741 gcatggaata agggtttttc gtacatttcg ccttttgtga taaaaccttg aaattatcac
    16801 aatatcacaa aattatcacg attgggggta tgacaactga atgaaactga ttttttaacc
    16861 gccttgaaag agaaacgctg tgcatacagc gtatcgcaga caaggcttgc aatcatggca
    16921 aatatcagcc gtgaacattt gagccgtatc gaagcaggaa aggtaacgct gactgaggac
    16981 atgaaacata cgcttttaga agcggtggaa aaattcaatc ccgacaatcc catgttcctg
    17041 ctgtttgatt atgtgcgtat ccgttttccc acaatggata taaagcatat catcaagaat
    17101 atattaaagc tcaatatcga ctatatgctt cacgaagatt acggacatta taaatacacc
    17161 gagcattatc atatcggaga tgtgtttgtg tatgtatcgc aggacgagga aaaaggtgtc
    17221 ctgctagaac tcaaaggcaa aggctgtcgt cagtttgaaa gttatctgct ggcacaagag
    17281 cgaagttggt atgacttctt cacggacgca ttggttgagg gcggtgtgat gaaacgtctt
    17341 gatttggcaa tcaatgaccg aacgggttta cttgatattc cagagctgat acgaaaatgc
    17401 gagaatgagg aatgtatttc taaattccgt tctttcaaaa gctatggttc tggggaactg
    17461 gtaaagcata acgaaacgga taaatacggt atgggacaca cgttgtatat cggttcattt
    17521 tcaagtgaag tatacttctg ctgttatgaa aagaattacg aacagtacgc aaaacttggc
    17581 ataccgattg aagaagtccc cataaaaaat agatttgaga tacgtttgaa aaatgaacgt
    17641 gcctattatg ctgttgtgga actgctgaca cattatgacg cagaacagac agcattttct
    17701 attatcaacc agtatatccg ctttgctgat aaagaaccag acaagcgaaa aagcgactgg
    17761 aaaacaaatg tacgctgggc ttggttcatt ggcgaggggc gaccacccgt aaagctgaca
    17821 acaaagccaa aaccgtacac gttggagcgt acactcaact ggctacaaag gcaggttgca
    17881 ccgaccttga agatgttgaa gcagatagac aaggacaatc agacggagta tctggaaacg
    17941 attgaaaagc aggcaaagct gactgaacga cactaccaga taatcaagca acagacagcc
    18001 acggttgaag aaatggttgc caacgtaaag gagtgatgaa aaaatgtggg aattagtaaa
    18061 agactttctg ctggtatcat ttggaatggg tatcggcgta gttttaatgt gcattttaac
    18121 tgtcggtaaa gaagctgaca gatgtatgga agaaatcaaa gaaagagagg acaattaaat
    18181 gaatttcgga caaaatttat acacatggtt tttaagcaac gcacagagct tagtgcttat
    18241 ggcgattgtg gttatcggta tctacttagg atttaagcgt gagttttcaa aacttatcgg
    18301 cttcttggta gttgcccttg ttgccgttgg gttggtattt aatgctggcg gtgtgaaaga
    18361 tgtattgtta gagctgttca ataagattat cggtgcataa aatttattgt agtacatctt
    18421 ttttattgat ataattattt tattacaata aaagaggtgt tagtgattgg ataatataat
    18481 tgaaataatt gaatggtgca ataacaatac tggttttgct acaatagttc tctcaatact
    18541 gactttaata gtgagtataa ttgcaatttt tgtttcccta agtacagcga gacttcctta
    18601 caaaaagaga atgctcgtag aacgaggtac ttttataagt tcagacggta tcggttttca
    18661 tgtaactgct acgaacgtag gaaatagaca agtcaaaata tcatctattg gttttaaaat
    18721 caattcctat gtgtatataa ataaattcac tttacatgaa agtcaaattg tactaagtca
    18781 aggcaaaaca accagtcaat attatgaaat tcaagatttt aaaagagctt tagctgaaat
    18841 gaaagttagc aattatacaa aagtatatgc ttatgtaaaa gataccgagg gaacagagta
    18901 taaaaaatat ttttcaagaa tatctcaaat aatgaaacag taactcttag tagtttactg
    18961 tttttttctt acctaaaatt cagattggag tgatgaaatg aacgatattt ttaaggatat
    19021 gcaggcaaag gttggctgtg aatatctttc tgacctgccc tcttacaagc gtaaggtgtg
    19081 gcatgaaatg aaatgtctga accctgccga ctatgaagaa aaacagttag aagatttttc
    19141 aaaatatgtg tttggtatgt catgccagac tttaaaagat gtgatgaaac aacagaaagg
    19201 acgtgaggaa caatgcagga aacaagggta ctggtggaaa cgaaaagaac aactggcgaa
    19261 gaaacaacat catactggtt cgacctgccg atagaggttg ccgagtttga agaaaagtta
    19321 ggtgtcagta cagaaagtca agattaccgc attattgaaa aattgttgcc ttatgctgat
    19381 gaagtccatg agcatacaag cgtgtaccag ctcaatgaat tagattatat gtatcgccag
    19441 ctttcaagcg atatgcagga agaatataca atgcttcata cggtgtatga aaatttagaa
    19501 gcactttata tttgcaggaa tgtaattacg ctttatcccg actgtaaaag cgtgattgat
    19561 gttgcaaggc aaaagctgat gaatgaccct acattcaaac atttatccga ggactgtcaa
    19621 gaatactatt ttgactttga aacctatgct tcacacttgc aggaacacgg gaaattttta
    19681 gtaacggaac acggtatctt tgaattgcca gagtaagaaa agaggtggtg cttatgattg
    19741 atgatatggc agtttatatc gctaatcttg gcaagtataa tgagggctat ttagtcggtg
    19801 cttggtttac tttccctatt gacgaggaag atgtgaaaga aaaaatcggc ttgaatgaac
    19861 agtatgaaga atacgcaatc catgataccg ataacttccc gattgagatt ggggagtatg
    19921 tttccattga tgaactcaat gaaatgtatg aaatgataga ggaacttccc gactatatcg
    19981 tggattgttt ggacgaattt attggtcatt atgggacgct ggaggaagtc gtggaacaca
    20041 aggacgatat ttactattat cccgactgtg aaaccatgac agatgttgcc tattactaca
    20101 tagatgaatt gcaggcactt ggcgatattc cccctagctt gcagaactat attgactatg
    20161 aagcatacgg gcgagatttg gatatgggtg gttgcttcat tgcaacaagc cgaggaatgt
    20221 gcgagatacc atattaacgc tggaagatca gcgacaagca ttgttgctac tgacggcgta
    20281 tttctcttta agggacagtc tgaaacaggg ctgtcctttt ccatttagaa acttatgaga
    20341 ggagttgaaa gaacttgaaa aagattagaa gctacacggg tatctggaat gtagaaaaag
    20401 tcttgtatgc aatcaatgac tttaacttgc cctttcccgt tacctttaca cagattacat
    20461 ggtttgtgat tacagaattt atcatcattc tgtttgggga tatgccccca ctttccatga
    20521 ttgagggagc atttttaaaa tacttcggta ttcctgcttt cctcacttgg tttatgtcgc
    20581 agaaaacctt tgacggaaag aagccataca gctttttgaa atcacagata acctatgcgt
    20641 tgcgaccgaa aatcacttat gcaggaaaag ccgtaaaact gcaaaaagaa atattcaacg
    20701 aaacaatcac ggcagtaagg agtgtgagtt atgttcccga ttaaatatat tgacaacaac
    20761 cttgtctgga acaaggacaa tgaggtgttc gcctactatg agctgatacc gtataactat
    20821 tctttcttat ccgcagaaca gaaatttatc gtgcatgaca gtttccgaca gctaatcgca
    20881 cagtcccgtg agggtaaaat tcatgctttg cagattgcca cggaaagctc tataagaagt
    20941 atgcaggagc agtcaaaaag attggttacg gggaaattaa aggaagtcgc ttaccagaag
    21001 atagacgaac agaccgaagc gttagtatca atgattgggg acaatcaagt ggactaccgc
    21061 ttttttcttg gctttaaact catggttacg gaagaacagc tcaatctgaa gaaccttaaa
    21121 aaatcggcgt ggctgacgtt caaagagttt ctccatgaag tgaaccacac gctgatgaac
    21181 gactttgttt ccatgccgaa tgatgaaatc aaccgttaca tgaaaatgga aaagttactg
    21241 gaaaataaaa tctcccgtcg ctttaaggtt cgccgtctgg aaatcaatga ctttgggtat
    21301 cttatggaac atctttacgg cagggacggt atcgcctatg aagattatga gcaccagcta
    21361 ccaaagaaaa agtataagaa agaaacactt atcaagtatt acgaccttat ccgcccgaca
    21421 aggtgtgtga ttgaggaaag ccagcgatat ttacgattgg aacatgagga caaggaaagt
    21481 tatgtgtcct attttaccgt caatgcgatt gtaggcgagc ttgattttcc gtctagtgaa
    21541 atcttctatt tccagcaaca gcaatttacc tttcccgttg atacgagcat gaatgtagaa
    21601 atcgtggaaa accgaaaagc attaacgacc gtccgcaaca agaaaaagga actgaaagac
    21661 cttgataatc acgcttatca agctggaagt gaaaccagct caaatgtagt agacgcttta
    21721 gacagcgtgg acgagctgga aaccgattta gaccagacaa aagaaagtat gtataagctc
    21781 tcttatgtta tccgtgtgtc tgctgatgat ttggacgaac tgaaacgccg ttgtgatgaa
    21841 gtcaaagatt tttatgacga tttgaatgtg aagttagtac gtcctgctgg ggatatgctg
    21901 gggcttcatt ctgaatttct accagccagc aagcgatata tcaatgatta tgtgcagtat
    21961 gtaaaatcag acttcttagc tgggcttggt tttggtgcga cacagcagtt aggggaaaac
    22021 acgggtatct atatgggcta ttccgttgat acgggaagaa atgtatatct gcaaccgtcc
    22081 cttgccagtc aaggcgtaaa aggtacagtt acaaatgccc ttgcttctgc ttttgttggt
    22141 tcgcttggcg gtgggaagtc attctgtaat aacctgcttg tgtactattc tgttttgttt
    22201 ggcggtcaag cggttatctt agacccaaaa tcagagcgtg gaaactggaa agaaacactt
    22261 ccagaaatcg cccatgaaat caatatcgta aacctaacaa gcgacaagga caacgcaggg
    22321 cttcttgacc cgtttgtgat tatgaagaat gtgaaagacg ctgaaagtct ggcaatcgac
    22381 atactaacct ttttaactgg tatttcctct agggacggcg aaaaattccc cgtacttcgt
    22441 aaggcggtgc gttctgttac ccagagtgaa acaaggggct tgcttcatgt gatagacgag
    22501 ttacgctgtg aagataccgc aatttcacgc aatatcgcag accatatcga cagctttaca
    22561 gactatgatt ttgcacatct tctgttttca gacggaacgg tggaaaatgc tatcagttta
    22621 gataatcagc tcaatatcat tcaagtagcc gaccttgtac tgccagataa ggacacgact
    22681 tttgaggaat acacgaccat tgaattacta tcggtttcca tgctgattgt gattagtacc
    22741 tttgccttag attttatcca cagcgaccgc tctatcttca aaatcgtaga ccttgatgaa
    22801 gcgtgggcgt tcttaaatgt ggcacaaggc gaaattttat ctaacaagct ggtgcgtgct
    22861 ggacgagcta tgcaggcagg tgtgtatttc gttacgcaat cttctgggga cgtgtcaaag
    22921 gaaagtctga aaaacaatat cggcttaaaa ttcgctttcc gttctactga catcaacgag
    22981 ataaaacaga ctttagaatt tttcggtatc gacaaagagg acgaaaacaa tcagaagcga
    23041 cttcgtgatt tggagaacgg acaatgcttg ttgcaggact tatacgggcg tgtcggcgtg
    23101 gtacagatac accccgtctt tgaagaactg ctacacgcct ttgataccag accgcctata
    23161 cagagaaatg aggtggagtg atgaaagaaa ggatacagag tgcgttcaca aaaaagaaga
    23221 ttttccactt tctcaaaatg gctctgttcg tggtggcact ctcccttatc ctgctttcac
    23281 ttctggggac ggtggctcat gcgacggggc ttgtggacga tacgataaac gcagaaaatc
    23341 tatattccaa atatccgctt tccaactatc agcttgactt ctatgtggat aatagctggg
    23401 cttggttgcc gtggaactgg ctggacggta tcggaaaatc agtacagtat gggctttact
    23461 gtattaccaa cttcgtttgg actatcagtt tatatttaag caatgccacg ggctatgtgg
    23521 tgcaggaagc ctataaactt gattttatta acgatatggc agacagtatc gggcagagca
    23581 tacagacctt agcaggcgtt acttcaagcg gtttttcaac ctctggcttc tatgttggtt
    23641 tcctgcttct cattatcttg gtggtgggaa tttatgtagc atatacggga ctgattaaac
    23701 gagaaaccag caaggcactt tacgccgtta tcaactttgt tgtggtgttc attttatcgg
    23761 cttcttttat tgcctgtgct cccgattaca tcaagaagat aaatgagttt tcatcagaca
    23821 tcagtaccgc ttcacttgat ttgggaacaa aaatcatgct ccccaactct gacagcgagg
    23881 gcaaggacag cgtggacttg ataagggaca gcttattctc tattcaagta gaacagccgt
    23941 ggctactttt acagtttgga aacagcaacg cagaggaaat cgggacagac cgtgtagaag
    24001 ctcttgtatc ggcaaaccca gaggacgagg acggaaagac cagagaggaa gttgtcaaaa
    24061 cagaaatcga aaataacgac aacagcaatc tgacaatacc gcaagtcgta aaccgtttgg
    24121 gtatggtgtt cttcctcttg ttcttcaact tgggtatcac gatatttgta ttcttgctta
    24181 cgggtatgat gttgtttagc cagatacttt ttattatctt tgcaatgttt ttacctatca
    24241 gctttctact ttccatgata ccgagctatg aaagcatggc aaagcaggca atcgtaaggg
    24301 tgtttaatac cataatgaca cgggcaggaa ttacgctcat tgtaacggtg gcgttcagta
    24361 tttccagtat gttttataac atttccacag actacccgtt ctttatggtg gcgttcttgc
    24421 agatagtatg tttcgctggt atctacatga agctgggcga cttaatgagt atgttctctt
    24481 tgaacgctaa cgacagtcaa agcatgggac gaagaatttt ccgcagaccg tatctctata
    24541 tggcacatag ggctaggcgt atggaaagac ggcttgctgg tgcggttacc gctggcggtg
    24601 tcgctggtgc ggtggctgga agtaccgttt ctaacgcaag gacaacaagg aacaatttag
    24661 caaaagacaa ccgagggaat acatcttcca gcatgggaca acgtgcaggc tcaaaggtgg
    24721 gtgctgtctt agatacgaaa aataaagtca aggacaaagc aaacgccatg aaagaaacgg
    24781 tcaaagatat gccgacacag accgcttatg cggtgtattc cgcaaaggaa aaagcaaaaa
    24841 caagtgtatc ggacttcaag cgtggcatgg tgcaggaaca gcagtccaga cagtcgggac
    24901 gcttggaaaa gcaggaacag cataagaaga atatcgctga taagcgtatg gagcttcaaa
    24961 aagcacaaga agcaaggcag gcacgaaaga cggacggatc agcgacaacg ggagctaccc
    25021 gtccccatga acgaccagcc acagctccaa cttctaaaac aagcaagcca gtcaaaataa
    25081 attctgcgaa aaaacgtccg ctatcttcgg gggtttctga tgtgaaagct ccacaatcgg
    25141 cacagccagt acatagacag aatgtagaaa aagttcccgt atcagagaaa acacgtcaga
    25201 atttcaaggc agagcgtaca acaaaggcac atatcgtcca gcaaccaaag aaaataacgg
    25261 aaacaactga aaagaaccgt aacgcagtat ttacgaaagg acagaagaaa aaatgaaact
    25321 gaaacatatc gctcttatcg gcagtctgtt tcctatcctc ttttccatag tccttttctt
    25381 tggggtactg atgagtgcgg acagcgacga cgagaacagc aacttttctt cgggcattac
    25441 gggtatgaat ttatccgcag aagtcttgaa acatcagcct atggtggaaa agtacgctaa
    25501 agaatacggt atttctgagt atgtgaatgt gttactggct atcattcaag tagaaagtgg
    25561 cggtacggca aaagatgtca tgcagagttc agaaagtctt ggtttaccgc ccaactcgtt
    25621 agatacagaa agctcaatca agcaggggtg taaatatttt gcgtccctgc tttcttcctg
    25681 caaaaatcaa ggaataaatg atttgaatgt ggcgatacag tcctataact atggcggtgg
    25741 ctatgtggga tatgtggcag gaaaaggaaa gaaacatact tttaaccttg cagagagctt
    25801 cgcccgtgag aaatcgggcg gtaaaaaagt aacctacacc aacccgatag ccgttgcaaa
    25861 gaacgggggc tggcgttatg gctatggaaa tatgttctat gtggaattag tcaatcagta
    25921 tttaactgtt ccgcaggtat cgggagaact ggcacaaaag gtaatgaatg aagcattgaa
    25981 atatcaaggg tggaaatatg tttatggtgg tagcaatccc aacacttcct ttgactgttc
    26041 ggggcttgtg caatggtgct atgggaaagc tggtatttca ttaccgagaa ccgccaactt
    26101 gcagtatgac gcaacccaac accttccgct ttcgcaggca aaggctgggg acttggtatt
    26161 tttccattct acctataacg ctggttcgta tgtaacccat gtaggcattt atgtaggaaa
    26221 taaccagatg taccatgcag gcgacccgat aggatatgca gacttaaata gtagttactg
    26281 gaaacagcac ttaattggtg caggacgagt aaaacaatag aaaggacttg aaaatatgtt
    26341 taagaagaat aagaaaccga cagaaaatgt caaagaaaag aaagtgcgta ccgtgaaagt
    26401 gggtacacat aagaaaactg tgattgcatt gtgggtggtg cttatcgcaa gcgtgagttt
    26461 tggcgtgtat aagaatttta ccgctattga tatgcacacc gtacatgaaa cagaaaccat
    26521 acaattaaga cttactgata ccaacgggat tgaaaatttc gtgaagaact ttgcgaagtc
    26581 ctattacaca tggaataaca gcaaagaagc gattgaagca aggacgcagg caatcagcga
    26641 ttatctgaca aaggaattgc aggacttgaa tgttgatacc attagaaccg acataccgac
    26701 cagctctgcc gttactgatg taattgtatg gagtatcgag cagttgggaa cggacacttt
    26761 ttctgctacc tatgaagtag atcagcagat aaaagaggga gaacagacaa cagcagtcaa
    26821 ggcaacttat actgtaaaag tccatgtaga cgctgacggg gatatggtaa tcatacagaa
    26881 ccctaccctt gcaccagcaa tcgaaaaatc agagtatgag ccagagacac tagaaacaga
    26941 cgcaagtgtg gacgctgata ctgtcaatga cgctaccgct tttctggaaa cattctttaa
    27001 tctgtacccg acagcaacag aaaaagagct tgcctactat gtagctagaa atgtgcttga
    27061 acctatcggc aaggactatc tttattctga attggtaaac cctatcttta taaaggacgg
    27121 ggacaatgtg aaagtcaagg tagctgtgaa attccttgat aatcagacaa aggcaacttt
    27181 agtttcgcag tatgagcttg tgctacataa ggatagtaac tggaaaattg tagaataaca
    27241 tggaaatggg cttgtatcat aattatggta tgcaagccct ttttatattg aagtataaat
    27301 taacaataat tataattagg tacatgaatg gagggataaa atgttcaaaa ttggtgagtt
    27361 ttcaaaattg acacaagttt ctactcgtat gttgcgttat tatgatgaaa caggtttatt
    27421 aaaacctgct aaaattgatt cattaactgg atatcgtatg tattcagcag aacaaatctc
    27481 aactttaaat agaattatat atttacgtga tagtggtttc aatgtcgctg aaattgcatt
    27541 agccctcgat tgcagtaatg ataccattat agaacagctt gataaaaagt ctattgaaat
    27601 tatggataat atacaaaaag aacaagaaaa actaaagaaa attgagattg ctaaaaaaga
    27661 agtgttacat ggcaaaagtg aattgtattt taacataacc attaaagaga taccgagtta
    27721 ttgcgtttta tctttaagaa aagaaatacc aaattattat tatgagggcg aactctggaa
    27781 agaactctct gcttttgcta aagaaaataa aataaatatt tctgatgaaa cattctcaat
    27841 atatcacaat gaagaatata aagaaaaaga tgttgatgta gaattatgtg ccattgtaaa
    27901 taaaatagga aaagctacag gtgattttaa gtttcgtttt accaaaccag tttctatgat
    27961 ggcttgtact atggtatatg gagatttttc taatatttct tatgcttatc gttcatttgc
    28021 taaatggctt caagaaaaca gtcaatataa gatgaaaggt tcaagccgtc aaatagttca
    28081 tcgtggaccg tggaacgata aaaatccaga gaactatcta acagaaatcc aaattccttt
    28141 agaaaatctc ttgtaaaaaa tgataatctg ccttgacttt aacatggtgt taaggtttat
    28201 cgtttattta tcaagaagaa aacggaggaa aatataatga caacttataa agtaaattca
    28261 ataggtacta tctgcaacaa tgaaaaaggt gctttcattc aactagaagc accatatatt
    28321 ccagcattaa aggaattaga cggattcagt catatcaatg ttttatggtg gttcagcgat
    28381 tttgacaatg cggattttag aagcgtattg gaaacagacc aaccttataa aaaagctcca
    28441 gagaaaatgg ggatttttgc aacaaggtcg ccagtacgcc caaatccgat tgctttatca
    28501 gcagtagaaa ttatcgatat tgattatatg agtgggctga tacaaattgc ttatattgac
    28561 gctaatgaca aaacgcctat cttggatatt aaaccataca cgccgagttt ggatagagtg
    28621 gaaaatccgc aagttccaga ttggtgtagt cattggcctc acagcttaga agaatctgct
    28681 tcttttgcat gggaaagcga attcaatttt taaggagtga tgaaccatga gtttgcagga
    28741 ctacattttt gaaaaaccag taattgaaac agaacatctt attttaagaa caatgaataa
    28801 aaatgacata gatgatttga aagaatggat tagtgatatt tctttatacg agtattgggg
    28861 gaaacgtcct tcaaaaagcg aaaaaaatcc agaattactc tttgataagt caaaaaaatc
    28921 tactaaaagt tttcattggg gtattgtcca taaacaagat agcaaagtga ttggtgaaat
    28981 atggatatat cttattgaaa ataatagaat ggcaaaagtg gctttcagac tttctaagca
    29041 ttatcaaggt aatgggctaa tgagtgaagc tttgaaaagc gtaattgaat tttgtttttc
    29101 aaaaacagaa ttacaaaaaa tatggacaga tgtgcatatt ttaaatcatg cttcttataa
    29161 aacattagag aaagtaggtt ttcaaaggga aggacatatt cgtgagggga aaatggtaaa
    29221 tacctattgc gactattatc tatatgggat tttgaaatct gattatagca ctatctgatg
    29281 ttatggctaa tattgaagcg acgattgacg aagcaatgaa caatacagtt ccaaaggtct
    29341 atgatacgaa aagcattaaa agatatgtga tgaatttatt agatatggct cattaaaaag
    29401 taccaaagga ataaaagtta ccatatagac aaaattggtt ttttatatct tttgatttct
    29461 tataagataa tggcattgtg cgacaagcta tacaaaaaat tttcatgtat acttcgatta
    29521 taaattatac aaggaggtac tgatatgtac gcatatgaaa atattgaaat gtcattaaac
    29581 tatattgaac aacatttaag tgagaaaatc gaaacagaga aactggcgga aattgcctgt
    29641 ctttctacat tttattacca acgcttgttt aaaaggttag ttaagaaatc agttcaagag
    29701 tatatcaaac ttcgtcgttt agcaatggta atagcagaat tgaataattc agaggaaaga
    29761 atattagaca tagctttaaa atatggattt tcagaccatg ctaacttcac aagagcattt
    29821 aaagaagttt accatattac tcccgatgaa tacagaaaga atgttcctat gttaaataca
    29881 tttgacaagc cagaactttc ttcacgttat atcatgattg atgaaaatgt cccattgatt
    29941 gtaggaaata ttgttttaga aattcaaaga aaaacattaa gaacaaagga aacatatttt
    30001 ggttttgaaa aaacagtaaa tattgctgaa caaattcctg ctggagaaag tacaggaatt
    30061 gatgttccag gtcagctatg gcgcgaattc catgcaaaga aactgcaaac cgaaaatgcc
    30121 atttttaaca atgatattga acttggtgta tctcacttag caagtccaga taaaggaaca
    30181 ttcctttact ttgcaggagg tatggtttca tcaacagatg tatgtcctaa aaatatggta
    30241 caacatacat taccagcagg tgagtatatt gtatgcaaaa ttgaggcaga atcttttgaa
    30301 gaacttgtta ctactgcttt aaatcaagca aataagtatc tgtttgaaac ttggcttcca
    30361 aatcataatt tggttacaaa gccattcatg gcagaaaaat attacaaagg aaatgcagag
    30421 attaattata tggaaatatg gataattcct gtgtaaacag taaatgctaa ataggaatta
    30481 tctgacgtaa taaaaaggaa atagaaagct tataaaggag ggcatagtcc gttgtgataa
    30541 acataaaaga tataaattat gtacctaata ttagtgaaat aagcgtttat attgaaaact
    30601 ctgcatttga tgagttgtat cagcatatga acaatgaata taaagcagtt tgcaaaatcg
    30661 aatacagtaa agatgtctgg gcacgaggtt ggaacgtaaa gtttaaaaaa tctgggaaat
    30721 cattgtgtgt tgtctatccg aagaagaact actttactgt attggtggtt gtaggtaata
    30781 aagagaaaga agatgttgaa aacttgttac ctaggctttc tgaatatgta caggaaattt
    30841 atcgtaacac tagagaggga aatggacaaa ggtggcttat gatagactta aaatctaaaa
    30901 ataatgtata tcaagatgta ttaaggctta ttgaaattcg ttatgccata aaatgtgtaa
    30961 aaccgtaata aaaatatttt tcacatacgt aatctcaagg tattatcgtt gagacaagta
    31021 acctatataa ttatgggaaa tgagattttt agatacttaa gaaagtactt tagggggctt
    31081 gcgttatgga aagatttaag tacaattcca cacaatcaaa tacaatctaa taaagcttta
    31141 ctaaaaattg aatgcaatac agctttaaag gctcgtacaa actatatgtt ttgtgcgggc
    31201 ttttttcatt taataggaat ttggattcgt cagagagtat attataaaaa taaaacgata
    31261 agggtacctt aaaaataagg tacctcaggc tgtagacaaa gtcccggact ctcgtctggg
    31321 cttttcttat atttgagtta tccacaattc aactttttaa gagttatctt attaagaaaa
    31381 aagtgtatgt tttccacccg atagttttac ttctgtgata tttgcagatg ccactcaagt
    31441 agaaagtggt ggtacggcag aagatgtcat gcagagttca gaaagtctgg gactaccgcc
    31501 taattcctta gacacggaaa gctcaatcaa gcaggggtgt aagtattttg cgtccctgct
    31561 ttcttcctgc aaaaatcaag gtatcgacga tttgaatgta gcgatacaat cttacaacta
    31621 tggcgggggt tatgtgggct atgtggcagg aaaagggaaa aagcacactt ttaatcttgc
    31681 agagagcttc gcccgtgaga aatcgggtgg aaagaaagta atctacgcca acccgatagc
    31741 cgttgcaaag aacgggggct ggcggtatgg ctatggaaat atgttttatg tggaattagt
    31801 caatcagtat ttaactgttc cgcaggtgtc gggggaactg gcacaaaagg taatgaatga
    31861 agcgttgaag tatcaagggt ggaaatatgt gtttggggga agcaatccca acacttcctt
    31921 tgattgtagc ggacttgtgc aatggtgcta tggaaaagct ggtatctact taccgagaac
    31981 ggcacaaaca cagtatgacg caacccagca tatcccttta tcgcaggcaa aagctgggga
    32041 gttggtcttt ttccattcaa cctataacgc tggttcgtat gtaacgcacg tcggtatcta
    32101 tgtgggaaat aatcagatgt accatgcagg caatcagcgg ttatctaaca aagaaattgc
    32161 aggacttgaa tgttgatact attagaaccg atatactgac tagccccaca gttacagatg
    32221 tgattgtgtg gaatatcgaa cagtcgggaa cggacacttt ttctgctacg tacgaagtag
    32281 atcagcagat aaaagaggga gaacagacaa caacagtcaa ggcaacctat acggtaaaag
    32341 tccatgtaga cgctgacagg gatatggtaa ttattcagaa ccctaccctt gcaccagcaa
    32401 tcgaaaaatc ggactatgag ccaaagacac cagaagcaga cgcaagtgta gacgctgata
    32461 ccgtgaatga cgctaccgca tttctggaaa cattctttaa gctgtacccg acagccacag
    32521 caaaagagct tgcctattat gtatttggaa atgtgcttga acctatcggc agggactacc
    32581 tttattcaga attggtaaac ccagtattta caaaggacgg ggataatatg aaagtcaagg
    32641 tagctgtaaa attccttgat aatcagacaa aggcgacgca ggtattgcag tatgagctta
    32701 ttctacataa ggatagtaac tggaaaatta ttatgtaaga cattttgttg gcaggatcta
    32761 ttttcggata tagtctgcct tttctgtagc aaatgtactg cactgacaag ctatttcatt
    32821 gtattttaag caatggaatc tttcttgaca attttacctt aaatcatata tttatagcta
    32881 ttgactttct gcttgcaata agctataata tgactataaa atcaaatcag tcatatagga
    32941 ggtatagtgt atgtataaag aagttggcat ttcttcaaat aatgttccga taacactttc
    33001 tatttggtat acagaaagaa gtagtcctac cattgttttt ttgccaggta ctatgtcaca
    33061 ccctctcatg tatgagaatt ttctatgtgg attgagcgaa agagggttta atataatagg
    33121 tgttcactat ctttcacacg gaaaaagccc taaaatcaaa aaaaattata taatggaaga
    33181 tatgttgcat aatgtatatg acgcaacaac ttacggaata gaaaatttcg gtgaaaacat
    33241 agctgtcatg ggttccagtc aaggcgggat tttagctgca atggcggcag gtagggacac
    33301 aaggttaaaa gccgtttttc cacataatat catgctgact acattaaaag aaactatgag
    33361 cttaacaaaa tatcccgcat ggtcgcaccg ttttatgggg ctgatacagt gggggtttcg
    33421 tgtaggtgga aaattgctgc ctaactacaa ggttccagga gacgcttatt tagactatga
    33481 cagagtattt tatagtgaac aggcaaaaca agattgctta aatgacccta tgcttttacc
    33541 ctattatcct ttaagatttg tcgccagctt atttaatgct gacttatcgt gcctggagaa
    33601 tggggctata caatgtcctg ttattttaat tactgccaaa ggcgacccgc tttttagctt
    33661 tgattatacc aacaaagtat ttgatttaat tcatgtaccg caaaaggaat tacttgcttt
    33721 agagcttaat cgtcacatga tttttaatga ggacacggac actacaataa acgcattgga
    33781 agctaccttg cataaatatc ttggatagca tattgattat ataatcagta aatgttatac
    33841 ttttcatgac gatataaata ttttagtcat aaggaggtta tgagtttgcc acgatttacg
    33901 gaacaagaaa aagaaatcat aaacagtaaa cttttgatag agggagaaaa gctgtttgca
    33961 ttacacggct taaaaaaagt aacagttgat gatttagtag ctgctgtaaa catatcaaaa
    34021 ggttctttct atgcttttta tcccagtaaa gaacatctat atgttgaaat caattttcgc
    34081 ttacaaaaag agttatttac aagtatagaa acaaccatta aaaagaaaaa gtataagaac
    34141 catagagatt tagcaaagga cgttatcatg ctaagtttaa cgggggtgat tacttcgcct
    34201 attctttcac agattgattt atctttaatg gactatctgc aaagaaaaat atcgtcagat
    34261 atttttgaaa atcatatgta cagtgatatt cgtatattgg agatattgga agatttaggg
    34321 gttgtatttt tggttccgca tacggttatt ataaaatccc tgtattctgt tttatcctgt
    34381 ttggaacaat tcaaagagga cgaagaactg aatatgatac aaaatctttt agtgaatggt
    34441 attattcagc aagtagtagc agagtaacaa ttttgtttgg ttatttgaca ggcaggtagc
    34501 caaacaaatt ttttacagtt tatatgacta tatatctata aacgtcataa agttaaagga
    34561 ggaatgaata atgattgatg taaaaaaact gtattttagc tatactgaca aaccgtttgt
    34621 ggagaatgta agtttccatg ttgggaaagg agaaattttt ggttttttag gaccctctgg
    34681 tgctggaaaa tcaactattc aaaaagtttt aactgggctt aatacaagat ataaaggcag
    34741 cgtaaaagtt gcaggtacag aaataaggga gcatacaaat cagttttatg aaaatattgg
    34801 ggtagacttt gaattttcaa cctgctacga gaaattcaca gcacgacaaa atctcgccta
    34861 ttttgcttct ctatatgaaa agcaacctcg gtctattgat gaattattgc atatggtagg
    34921 cttggaaaat gacggggaca aaaaagttgc tgacttttcc aaaggtatgc gttctcgctt
    34981 gaatttcatt aaagcgttag tccatgaccc tgatatatta ttccttgatg aacctacaag
    35041 cggtcttgac cctacaaata gtcgtttaat gaaagatatt attcttgcgg aaaagaaacg
    35101 cggaaaaacg attatcatta ctactcacaa tatgtttgac gcaacagagc tatgtgacca
    35161 agtagccttt atcgttgccg gaaaagtcag tgcattggat agcccgcata atttgattat
    35221 gtcaagggga gctgccaaaa tacaatatac ctactatgat aaaggcgaga aaaccggaga
    35281 atgtctgctt gatagaacca cagaagataa gttgttaaaa agtttaattt ccgaaaaccg
    35341 tttgctttct attcatagta gtgagcctac attaaatgat atttttgttg atattacagg
    35401 gaggacactt caatgagatt gggacgttta atttgtggtg atatacattt ccaatggaaa
    35461 tatggtttct attttatcta ctttatatta acagttttat atgtgtgcgg gattgccgct
    35521 ttgccggaac attggaaaac ggatattgct tctattatga tctactctga cccagcagca
    35581 atgggattgt ttttcatggg agcgattgtt cttttggaaa aaagtcaaaa ggtattaaat
    35641 gctatggtgg tatcccctgt aaaaatatcg gaatatatac tttcaaaaac agttgcgttg
    35701 attgctattt ctacggtaat cgcattgata ttaggggtag tttccggcag caatcattta
    35761 cttggtatca cagttggtac ggctttgacc tcagcaatct ttactatgct tggaattatt
    35821 gcagctacaa aaatttcaaa cttgaaccaa tttctcattg tgattatgcc gatagaaatt
    35881 gtttgctttg taccacctat tgttggtctg tttgtgaagt taccatatct gttccgcttt
    35941 tttccattca cagcttgcat gaaccttata actggaaaaa gcgttttact atcgtttgat
    36001 atggtacttg taattgctac gttgattatc ctctacatgg ttgcgcgaca tacagttaag
    36061 catatgtgga aaagtttagg aggtgtaaag ctatgaaaag gatattatcc agtaccatac
    36121 aggtatttag acaaatcaag agcgacccta tgatgtttgc agcttgtttt acgccttttg
    36181 tcatgggagc tttgattaaa ttaggtattc cctttcttga aagaataaca gatttttctt
    36241 tgcaagcata ctatccgatt tttgatttac tactttctat catggctcct gtattactct
    36301 gctttgcatt tgcaatgatt acattagagg aaatcgacga taaagtgtca cggtactttt
    36361 cgattacccc tcttggtaag tcggggtatc tctttacaag gttgggagta ccctcgatta
    36421 tttcggcggt catcgctttt attgtactgt tacttttttc attagaaaag ttgtccattg
    36481 gaatgacaat atgtttggcg cttctcggct cagtacaggc aattattgtt tcacttatga
    36541 ttatcacatt atcaagcaat aaattagagg gtatggcagt tacaaaacta gctgcactta
    36601 cattgctcgg aataccagcc ccctttttta ttgatagtta ctaccagttc gcggttggct
    36661 tccttccatc attttgggta gcgaaagcca tgcagaatga ggcggttctt tatttctcta
    36721 taggattggt ggtagcttta gtctggtact acttccttac aaaacgtctg tttcggaagc
    36781 tggcaggata aggaggcata ttatggaaaa gggtagactt ctgcaaaacg gaaaagtaaa
    36841 tagagctatt caggtttcca aaatcacact atttatcaac ctaagtttat cgttacttaa
    36901 atttgctgcc ggatatatag gaaagtcaag tgccatgctg tcggacgctg tacactcggc
    36961 ttctgatgta ttaagcacaa tcgttgtgat ggttggaatt aaaatatcag aaaaacagcc
    37021 tgatagagaa catccttatg ggcatgaacg tatggaatgt gtggcttcca ttattctctc
    37081 tgttgcattg gctataacgg gtgctggaat aggatattca ggtatcaaga aaatcttttc
    37141 aggacagtac aatacgcttt ctgtccctag tggaatcgca cttacggcgg cagtcttatc
    37201 cattgttata aaagagtgga tgtactggtt tacaagaaat gctgcaaagc atacaaattc
    37261 tgacgcactt atggctgacg catggcacca ccgctctgat gcactctcgt cggttggttc
    37321 cttaattggc attttaggag cgcgtttagg ctatgctatt ctcgacccta ttgcaagcgt
    37381 tgtgatttgc ggttgtatct taaaagctgc tttggacatt ttcaaagaaa gcattaacaa
    37441 aatggtagac catagttgtg acaatgctac tgaaacaagg attagggaag ttgtgcttca
    37501 gcagcaaggg gttgatggta ttgatgaatt aaaaacccgc atgtttggag cgaaaatgta
    37561 tgtagatatt gaaatattag ctgatggtaa tttagctctt tatgacgctc atagaattgc
    37621 tgagggagtc catcaaacga ttgaaaacaa ctttccacaa tgtaaacatt gtatggtaca
    37681 tgttaatacg aatgaacttc tctattctgc ataggacagg gaagatgatt taagactatt
    37741 gaaagggaaa gggggggaag taggttatgg aacaatactg gtggattatc attgttgtgg
    37801 ttttgattgt cgttggctta cttaaaattg taattatgaa gaagtataac caaaggagaa
    37861 attctaacaa tcgttcaaaa gatagcgagg acgacaagat gtattaaggt ttattgaaat
    37921 ctgttatgtt gtagaatgat gtttttagat actagaaagt gctttaaggg ggctaacgtt
    37981 aaggaaagct ttgagtacaa ttctttacaa tcaacagtct tttgcataga gaaatgtatg
    38041 taggaaatat ggtgcaaggc acaaaacacc agcaattgag aaatcaacct atgaaccaaa
    38101 ggcacactac attttatact acttgacaag taaacgaacg tttactaaag aaggaggttg
    38161 taaatgtctg acacaaaaga aaatatatta aaaattgcac tacacttatt tgcagtgaat
    38221 ggttatgagg ctgtttctgt tagtatgatt gcggaagcct tgggaatgac aaaaggagca
    38281 ttatataaac attataaaaa taagcaagat atttttgact gtatcgtaga acgtatgtat
    38341 caaattgata ataagcgagc taaggattat caagtaccag aaagcacaat agaggtaacc
    38401 cctaatgcat acagaaatac aactattgga agtctaataa catttactct tgcacaatac
    38461 gatttttggg tgactgatga atttgcgtct aattttcgca aaatgttaat actagagcaa
    38521 tatagaaatt taaaaatgaa tatgctttat caaaaatgtc ttgtaaatgg tcctatcagt
    38581 tatatagaag atatatttaa tgaaatgatt gaaaaaggtg ttcttcaaga gtgtgataca
    38641 aaacagcttg ctattgaata ttatgcgcca ttatttctat tgataagtgt agcagacgca
    38701 gttgaaaatt gttgggattt aaaaactcaa ttaactacac acgttaaaca atttgtcaag
    38761 aaatatatta taaatgagaa agtaaaataa tcctttattc tgtcctaaat ataaacctaa
    38821 ctttttaatt tcagcagaga aactcaaaat gaatattgtt ataagtcaca aatattaact
    38881 attaaaatat tacaataacg gagatgagaa aatggaatat attcgcagta aaaaatattt
    38941 tacacccgac ttacagacaa aaattatggg gccgaatcct ataaaattac aagaggaact
    39001 attacaaaat cataaaatcc cgtctggtag ctatgtctgt gaccttggaa gtggacaagg
    39061 tttaacttct gtatttcttg caaaagaata tggatttgtt gtttttgcag ttgacttatg
    39121 gagtgagcca aaagaaaatg aagaattttt caaaaaaatg ggattatctt ctgcacaaat
    39181 taaatctgta aaagctgacg ccaatgaact taaatttgag aaagagtttt ttgatgctgt
    39241 agtaagtaca gattcatata attattttgg acgtgaccct atatatttag atgaaaaatt
    39301 gcttccattt gttaagaaag gtgggtttgt ttatatcaca gttcctggca tgaagaaaga
    39361 ttgccatgag aaacttccag aagagttatt gttatcatgg acaccagaag aacttgatta
    39421 tatacatgat ataacatatt ggtctaacat aattagtcat tgtaaaaagg cagatttaat
    39481 ttcaatatat gaaatggaaa gtaattcgga agtttggtcg gattggataa agcaaaataa
    39541 tgaatatgca ataaatgacc gtaaggcaat agaagctggc ggtggagatt atctgaattt
    39601 tattgctata attctacaaa aaaaataaat tctttagaac caaacactat aatttgcgtt
    39661 ctaatatgtt taccgattga catcaataat tttatctttg agggtaatga tttgaaaata
    39721 gaagaataat tccagtttgt aaaagtcaat aaatccaaag ataaatttgt atttgaaagg
    39781 agttttgaag aatgaaaaaa tatgagtatg tagaaattaa ctataatgcg aaaggagttg
    39841 tattcctttg tactgataaa cacaaagaaa ttattgacag ttacgcaaaa acggggtatc
    39901 actacgcagg gtttgtaccc acagaaattg acgcaaaagg ctgtatgcga aagatagatt
    39961 taatttttga aaaggaagat taacttattt tagggagttg gttctaatga taggacttaa
    40021 aagacgagat atgaattata ttatgaaagt tggtgctaaa gctcaaggtg tttctgtatc
    40081 tgaatttagg gctatgctac aagataccat agacaaaact atggaaagta cagacccaga
    40141 cgtgcaggca aattttaaac gatactttgg aaataagcgc ccaacaccag aggaatatat
    40201 ttatacgatt accaaaaaga caaaggttaa ggtttaaatg ttgtaaaggg ggcttgccgt
    40261 atgaaacgct ttatgtacaa ttcagtatat tcaagcccaa tttaacagca tactatttac
    40321 aattaaatac gctataccag tagctcgtac aaaatttata ttttgtgcgg gcttttttaa
    40381 tacccgaaaa gtttttttaa ttattttcat ttttttggaa gcaaatcaca ccttgctgta
    40441 cagatatggt gcggaaagag ggataaacac ttttcaattc atagctggaa aggaggtgag
    40501 attatgaaac cgtctgactt ccaaaaaaca attcagtgtc agtttgaatg taagctgaaa
    40561 aaagttgtca aaggtattat ctggaactac cgtaaagaat taaaacgaag aaaaaacaag
    40621 gaaatatctt ttagcgaatt accagacatt acggtagaaa gtctggcaac atgggacgac
    40681 tacgaaacaa actacacaat ttttaatgtg tgtggcatag atattaaagt ccttgatgat
    40741 gaacttgcag aagctctcaa acagctaccc gaaaaaaaac gtaacaatgt attgatgtat
    40801 tacttcttgg aaatgagtga tacagagatt gcagatatta tgaattatac cagaggggct
    40861 gtgttcaaac acagacaccg tgcactagaa actatgaaag agatactaaa ggagaagaaa
    40921 tagaaatgaa gaaaaagtac aagcacccgt cttattattt gatttcatca gctatggatg
    40981 gggatgaaac tgcgattgag aaattactgg cattctatga cccgtacata tcaaagtgtt
    41041 gtttacgtcc tttatatgat gaatacggca atgtttatat tgttgttgat atggaattaa
    41101 agggacgtat tcgagaagcc ttaaccaaaa tgattttaga ctttgagatt gatatagata
    41161 tagaaaccga agaagaataa gcagatacca gcagggcgtg attggttaaa ttcccttctt
    41221 tcctgccctg ctcctgcttt tatacatgga agcctacggc tttcgccgat tgttcttaga
    41281 caactgaata aagcagacag atacgtttgt gatacagcga gccacgggga ctgtacgcca
    41341 tgacctttcc gacaagaaag cgagcgaccc acgcagttga tccgagagcg acttgtggaa
    41401 tggtcgtttt gccatgacct gctatcacag aataatgata cgtccgcatg gtgcggttct
    41461 gcccacaaga atgggaatag ttgagatact aacggagctt tccaaagccg tctgtctgct
    41521 tatgataaga cacacagaaa ggagttgttg caacatgaac aatactgatg tgccgatttg
    41581 ggaaaaatac acccttagta tagaagaagc gtcccgatat tttcgtatcg gggaaaagaa
    41641 gttgcgtaaa cttgccgagg aaaatctgga ctctggttgg gttatcgtta atggaaaccg
    41701 tattcagata aagcgaaaac agtttgaaaa aattattgat acactggacg aaatctgatg
    41761 tcttagagcc gtagatatgg tataatatta gtagcgtatc acggctcatt ccatgacgga
    41821 aaggagcttt acactatgtc aaaaatgaaa agacaagaca gtaaaggacg caatttgcgt
    41881 cttggagaga gccagagaaa agacggaaga tacgtttata aatataccga tatattcggg
    41941 aagccacagt ttgtatatgc ttggaaactt gtacccacag acaaaacacc tgctggaaaa
    42001 cgtgatgata tatcgttgag ggaaaaagaa aagcagataa aaaaagacct tgacgacggt
    42061 atcgacacag tgggtggtaa aatgacagtc tgccagcttt acgaaaagaa gaacaatcaa
    42121 agaaagaaca tcaagagaaa cacaaaaata ggacgacagt atcttatgaa cgcactggaa
    42181 aatgacccat tgggtatgag ggcgattgat acggtcaagc agtccgacgc taaagaatgg
    42241 gctatcagaa tgaatgacaa aggttattcc tacaaaacga tagataacta taagcgttcc
    42301 ttaaaggctt cattctatat ggcgatacaa gacgactgta tcagaaaaaa tcctttcaat
    42361 ttcaagttga gtgatgttct ggaagatgat acggaagcaa aggttatcct aacaccagag
    42421 caggaagaaa aactccttgc ctttatggaa acagataatg tctacagcaa gtatcgtgat
    42481 gaagttattc ttctgctgga aacgggactt cgtatctctg aattatgcgg actgacaaca
    42541 cacattgata tgcttaaccg tgtgattaac atagaccacc agctattgcg tgatacagag
    42601 gtgggttact atatttccac acctaagaca aaaaacggga agcgtgagct tccattgaca
    42661 gaaagagcat atcaggcact tgaacgaatt ttaaagaacc gaggaaaagc acaaccgctt
    42721 gttgttgacg gttacagcaa cttcttgttc ttgaaccgtg agggattgcc aaaggtggca
    42781 ggaaattatg aaagtatggt gcgtgggctg attaagaagt acaacaagac gcataaggac
    42841 aagttgccaa acatcacgcc ccattcattc agacatactt actgtacaaa catggcaaat
    42901 aagggaatga accctaacac cctgcaatac atcatggggc acgcaaacat aaccatgaca
    42961 ttagggtatt atgctcacgg tactttccag tctgcaaagg ctgaattaga aaagctggct
    43021 tcttaatatc gaaaatgtat cttactactt atttactact tttggttgca ttttcatgct
    43081 ggtaaatgcc atcttatgcg aggtatcttc caaaaggaaa atgccaataa agcccgaaaa
    43141 tatgggatat atcggcattt accaacttat gaaaagataa tcagaaattt agtaagtttt
    43201 tatataataa aactgactgt aatttaattg gcaaagtcta gaagaatagg ctttatcaat
    43261 taaattacag tcagctattt tataataatt tagtttagat atcgttagta tttcacattt
    43321 ataacaatta ttaatgatat taatatattt attattcttt attaaaataa tatcctgctc
    43381 ctcttattgt tataattttc tgaggatttt tataatcttt ctctattttc tttcttaatc
    43441 cattcactgc aactaccaca ctatagttat cctctaagta ttcattcatc catacatgtt
    43501 catatatctc tttagtagtc aaagtctttt caggattctc tataaaaaag tttaacaact
    43561 tattttctgt tggagtcaat tttattattt tatcttctaa ctttattact ccactagttc
    43621 tattatattc ctctaatttt gtattatttt cattattatt tacatatgag ttcattcttc
    43681 ttaaaatagc ttttactttt gcgtataata tttctaaatt tagaggcttt attaagtaat
    43741 cgtctccacc agaatttaaa gcatttaaaa tgctttggtc ttcatttata taagtcatat
    43801 aaacaattgg acaagtagtt acatttctaa tttttttaca aagagtccat ccatcaccat
    43861 cacttagaat tatttctaaa aatattaaat catatttatt gctaaatatt tttccaatag
    43921 cttcttcaca attatatgct aaatctatac ttattccttt ttcctccaga aatcctttta
    43981 gcttctggca attatccttg tctccatcta ttatcaatat cttagtttgc attataatca
    44041 ctcccaaata tcattattct tttatattaa ataagctatt attataaaaa acatagactt
    44101 aattttattg taaaaacagt tccttttcct agttctgaat ctacaaatat ttctccacca
    44161 tgctctctta taatggattt gacaatatat agccctaacc cttgcccttt tggctcatta
    44221 ttgtctcttt ctgtaaagaa tctgtcaaat acctttggta tattttctgg aggtatacct
    44281 tttccattat cagaaatttt aataattgca aatccatctt ctttatccat tgataaaatt
    44341 atctttccat caaatggagt aaaggtcaaa gcattataaa ttaaattctc aaatgctctt
    44401 gtaagcatct ctttatttat ctttgcatat atttttttct ccaatacgta agtttcaaag
    44461 tttacaccac atgcttgtgt atctggcaaa ttattttcat atatctcttt aataaaatta
    44521 ttcagacata ttttttcact aataccaatg cttttatctt gtgatgtaaa ttcttgcaaa
    44581 ctacgtactc tattttgtac ttcatttgac ttttgattta tagcataaag ataggttctt
    44641 gtctcttcat ctacatgaat attaccaacc tttatcaaat ctataaatgc ttgtatagcc
    44701 gaaacaggtg cctttaaatc atgtgctaca tctgccaaga atcttttacg ttcatttaac
    44761 aatgttgtaa gttcattagt tctcacttca acttcatgtt caagattcct tgtaagtttt
    44821 tcattctctt ttattattat tctattcctg tttatcatca taacagaaaa taaaataaca
    44881 attacaaacc cacaatattc tgtttgccac cctgtaaata taggctcaaa tctattactc
    44941 gtaactgcat caaataatat gctaattcca aaaacagtgt ttactgaaac taaaatataa
    45001 gaacttatat gctcagagca tgtcttttta tatattgaaa ccagcgaaga cattaatata
    45061 tatataaata caatatattt atatatgtct attaacttcc catatatgtt gacaaagtac
    45121 atatcatatg gaagaagaat aattggtatc actatagata tgatactcat tgtaattcct
    45181 aatggtaaaa ttaaaaatct atacacttta ttttcttcta ttttggatat acttccattt
    45241 atacaaataa tacataaaac taccataaaa tatgacaaat cttcaaccgc atatgtaaag
    45301 cttaccaaag gcagtcctaa caaatgtaca aagggatagg atacatggat ggcaaagaaa
    45361 acacacatac atccaaaata taagtaaatt ttacttttgt ttgaaagaaa ccatacagac
    45421 aatgaaaata tagctattgc aaaagttgtg aagcaaatta tactataaaa aagtaatcga
    45481 taaaacatca tattacttat gtcctttgtt tctcctaatg caggcggata atacaaacca
    45541 ctataataat gtgaaaaatt cgatacattg attataattt ctgtattttt ctttgcaata
    45601 aaacttacta ctgaattttt aattttgggt ctatattttt ctgttcctac atctcctaat
    45661 ttagaaacca tttctccatt tatccatact tcacttgcgg aaaaaatctc aggaagctct
    45721 aaacttagta gcttatcctt gccttcattt tttattctca tacgataagt agcattccca
    45781 tatggtgact gattcccctt tctcatagaa aaatctggat attgacctat aaaaacatat
    45841 tctggtttta tatcttgctc tgtaaaatca ttcggtttaa atattttatg attataaaac
    45901 tcccagtcat ctatcaaaaa gacaagtttc cctttagtga aagtatcatt atctatagtt
    45961 aaaattccat tacttgctct aatagactta tatgtatatt tattatcata tttatacaga
    46021 agatttaatc ctgttattgc caacagagag actatcaata caatgagagt tccttttagt
    46081 atattatact ttttcattgt atcaaccaca tctctttcat agtagtatta aataaaacaa
    46141 gaactattta ttaattacag tatctcactt atggtacaaa cttatatcct tttccccata
    46201 cggtttgaat gtattgatgc tccctgctta aagaattaat tttttttctc atctgtgcaa
    46261 tatgaacctg cactgtatgt tgatctccaa tatcaggcat tttccaaact tggttatata
    46321 tctctatctt tgaaaatact tgttctttgt tttcagctag aaacaataat atttcaaatt
    46381 ccatagtagt taaatctata ctttctccat caaaagttac ttttcttgaa tttacattta
    46441 ttactaacgc tgaaaacttt aacacattag gaactgatgt aatatttttg tactgcctca
    46501 tacgagcata tattctaagt tcaagttctt ttaaactata aggttttgtt atataatcat
    46561 cccccccagc taaaaatccc cgaacttttt cttcttcatg ctcataatta gataaaaata
    46621 ttataggtat gttaaccttt tcttttatat cattgcacat tttatatcca tctatatctg
    46681 gcaatgaaat atccaaaatt atacagtcta aaactatact ctctactata tccatagcat
    46741 ctttagcagt ctctgatgta tatacattaa aacctatatt agaaaaataa atttgattta
    46801 tgtttaaaat atcaacatca tcatctacaa ccagtaaatt atacattaca ccactccatt
    46861 caaaggtatt ttaattataa ttccaagttt tatagttatt acaaattata atgttttatt
    46921 ttcatatata actggtatta ttattttata atatctttac aaactagact ataatactta
    46981 agtatttttg aataattctt gaatattgta ttaaacatta agattcatta ttaaatacta
    47041 attaaagtct ccgattgttg gagaatggag cttaaagtca ttttcacgta taaatttatt
    47101 atttttctag aaacttgtct attggctagt ttgagtattg atttatatga tttatatggt
    47161 tataatataa aataaattgt attattttgg taaatatatt gttacaaaag gtttatattc
    47221 tgcatctcaa tgtttataaa aactttattt tcttattata atactattat tcttttaaaa
    47281 aaatttccct tttccaacaa atggctactt taaaagccaa aattttacct atcaataaaa
    47341 atcttaacga ctaatactct tattttcaaa ttttgcaaca tatttgttaa ttttaaaaca
    47401 aaaaaagact atgcaatata aaaattatat tcatagccgg ttgcaccact aactccatat
    47461 atctctagat gcccaataat aagatatact tctcagtttc taaaatattt ttattaaaaa
    47521 tcttttatat attttcataa atttcttttt tattctaaca ttgtaatatt attttgtcaa
    47581 tattt
//
LOCUS       DAEORZ010000011        47458 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEORZ010000011 DAEORZ010000000
VERSION     DAEORZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.011"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            262..1338
                     /locus_tag="KRH39_000579"
     CDS             262..1338
                     /locus_tag="KRH39_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG8510503.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1455..2297)
                     /locus_tag="KRH39_000580"
     CDS             complement(1455..2297)
                     /locus_tag="KRH39_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG8510504.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2481..3053
                     /locus_tag="KRH39_000581"
     CDS             2481..3053
                     /locus_tag="KRH39_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8510505.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3216..3488)
                     /locus_tag="KRH39_000582"
     CDS             complement(3216..3488)
                     /locus_tag="KRH39_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510506.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3615..4487)
                     /locus_tag="KRH39_000583"
     CDS             complement(3615..4487)
                     /locus_tag="KRH39_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG8510507.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4783..7890)
                     /locus_tag="KRH39_000584"
     CDS             complement(4783..7890)
                     /locus_tag="KRH39_000584"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBG8510508.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7983..8215)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8390..8917)
                     /locus_tag="KRH39_000585"
     CDS             complement(8390..8917)
                     /locus_tag="KRH39_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBG8510509.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8952..9737)
                     /locus_tag="KRH39_000586"
     CDS             complement(8952..9737)
                     /locus_tag="KRH39_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBG8510510.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNCKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9747..10004)
                     /locus_tag="KRH39_000587"
     CDS             complement(9747..10004)
                     /locus_tag="KRH39_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBG8510511.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(10023..10481)
                     /locus_tag="KRH39_000588"
     CDS             complement(10023..10481)
                     /locus_tag="KRH39_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBG8510512.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10521..11225)
                     /locus_tag="KRH39_000589"
     CDS             complement(10521..11225)
                     /locus_tag="KRH39_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBG8510513.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11235..11870)
                     /locus_tag="KRH39_000590"
     CDS             complement(11235..11870)
                     /locus_tag="KRH39_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510514.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVNTLVLKNEKVDAK
                     IHVKGSIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12251..13291
                     /locus_tag="KRH39_000591"
     CDS             12251..13291
                     /locus_tag="KRH39_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8510515.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13754..15109)
                     /locus_tag="KRH39_000592"
     CDS             complement(13754..15109)
                     /locus_tag="KRH39_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBG8510516.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(15135..15404)
                     /locus_tag="KRH39_000593"
     CDS             complement(15135..15404)
                     /locus_tag="KRH39_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG8510517.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(15694..17649)
                     /locus_tag="KRH39_000594"
     CDS             complement(15694..17649)
                     /locus_tag="KRH39_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510518.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYENLEKREDFNITGKFKTEMLNKEKIIINDDVV
                     PSLNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(17832..19307)
                     /gene="spoIVA"
                     /locus_tag="KRH39_000595"
     CDS             complement(17832..19307)
                     /gene="spoIVA"
                     /locus_tag="KRH39_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBG8510519.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQVEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(19625..20644)
                     /locus_tag="KRH39_000596"
     CDS             complement(19625..20644)
                     /locus_tag="KRH39_000596"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG8510520.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(20675..21289)
                     /gene="plsY"
                     /locus_tag="KRH39_000597"
     CDS             complement(20675..21289)
                     /gene="plsY"
                     /locus_tag="KRH39_000597"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBG8510521.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(21324..22643)
                     /gene="der"
                     /locus_tag="KRH39_000598"
     CDS             complement(21324..22643)
                     /gene="der"
                     /locus_tag="KRH39_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBG8510522.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(22677..24005)
                     /locus_tag="KRH39_000599"
     CDS             complement(22677..24005)
                     /locus_tag="KRH39_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBG8510523.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(24243..24443)
                     /locus_tag="KRH39_000600"
     CDS             complement(24243..24443)
                     /locus_tag="KRH39_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510524.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(24445..25377)
                     /locus_tag="KRH39_000601"
     CDS             complement(24445..25377)
                     /locus_tag="KRH39_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG8510525.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(25422..26354)
                     /locus_tag="KRH39_000602"
     CDS             complement(25422..26354)
                     /locus_tag="KRH39_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG8510526.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(26409..27698)
                     /locus_tag="KRH39_000603"
     CDS             complement(26409..27698)
                     /locus_tag="KRH39_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8510527.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(27727..28419)
                     /locus_tag="KRH39_000604"
     CDS             complement(27727..28419)
                     /locus_tag="KRH39_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8510528.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(28434..29195)
                     /gene="pgeF"
                     /locus_tag="KRH39_000605"
     CDS             complement(28434..29195)
                     /gene="pgeF"
                     /locus_tag="KRH39_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBG8510529.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(29192..29653)
                     /gene="nrdR"
                     /locus_tag="KRH39_000606"
     CDS             complement(29192..29653)
                     /gene="nrdR"
                     /locus_tag="KRH39_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBG8510530.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(29675..29938)
                     /locus_tag="KRH39_000607"
     CDS             complement(29675..29938)
                     /locus_tag="KRH39_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG8510531.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(30042..30815)
                     /gene="sigG"
                     /locus_tag="KRH39_000608"
     CDS             complement(30042..30815)
                     /gene="sigG"
                     /locus_tag="KRH39_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBG8510532.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(30907..31629)
                     /gene="sigE"
                     /locus_tag="KRH39_000609"
     CDS             complement(30907..31629)
                     /gene="sigE"
                     /locus_tag="KRH39_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBG8510533.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(31654..32526)
                     /locus_tag="KRH39_000610"
     CDS             complement(31654..32526)
                     /locus_tag="KRH39_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBG8510534.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(32816..34090)
                     /locus_tag="KRH39_000611"
     CDS             complement(32816..34090)
                     /locus_tag="KRH39_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG8510535.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(34374..35531)
                     /gene="ftsZ"
                     /locus_tag="KRH39_000612"
     CDS             complement(34374..35531)
                     /gene="ftsZ"
                     /locus_tag="KRH39_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBG8510536.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(35721..36074)
                     /locus_tag="KRH39_000613"
     CDS             complement(35721..36074)
                     /locus_tag="KRH39_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBG8510537.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(36173..36847)
                     /locus_tag="KRH39_000614"
     CDS             complement(36173..36847)
                     /locus_tag="KRH39_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG8510538.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQGNNGNK
                     VNLFAK"
     gene            complement(36852..37550)
                     /locus_tag="KRH39_000615"
     CDS             complement(36852..37550)
                     /locus_tag="KRH39_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG8510539.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKAIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(37573..38313)
                     /locus_tag="KRH39_000616"
     CDS             complement(37573..38313)
                     /locus_tag="KRH39_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBG8510540.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(38583..39809)
                     /gene="murG"
                     /locus_tag="KRH39_000617"
     CDS             complement(38583..39809)
                     /gene="murG"
                     /locus_tag="KRH39_000617"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG8510541.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(39827..40957)
                     /gene="spoVE"
                     /locus_tag="KRH39_000618"
     CDS             complement(39827..40957)
                     /gene="spoVE"
                     /locus_tag="KRH39_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBG8510542.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(41009..42364)
                     /locus_tag="KRH39_000619"
     CDS             complement(41009..42364)
                     /locus_tag="KRH39_000619"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBG8510543.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(42383..43351)
                     /locus_tag="KRH39_000620"
     CDS             complement(42383..43351)
                     /locus_tag="KRH39_000620"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBG8510544.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(43392..44765)
                     /locus_tag="KRH39_000621"
     CDS             complement(43392..44765)
                     /locus_tag="KRH39_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBG8510545.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHAYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(44932..46911)
                     /locus_tag="KRH39_000622"
     CDS             complement(44932..46911)
                     /locus_tag="KRH39_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBG8510546.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(46923..47270)
                     /locus_tag="KRH39_000623"
     CDS             complement(46923..47270)
                     /locus_tag="KRH39_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510547.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
BASE COUNT        14559 a         8746 c         5049 g        19104 t
ORIGIN      
        1 ttataaaatt ttatttatta tttttcatta gtatataaaa agataaaata cataacttta
       61 tagacttaat ttattacttt tatagaattt tcacaataaa aagtcatttt acttccgtat
      121 ttaatgcaaa agtagagatt aaacttaata taaaaaatat ataacaaatt ttttttatat
      181 ttctagaaga aaagtatact cttttttgtt taataatgct aaaattgatt tgtattgaat
      241 tttgattagg aggaaattat tatgaaagct caaagattaa atgctgtact tgaacaaatg
      301 aaaaaagatg atatttctca aatgttagta agtgacccta cttctatatt ttatcttaca
      361 ggagtactaa tccatccagg cgaaagacta ttagcattat acttaaattt aaatggtaat
      421 aataaattat ttataaatga attattccca gtttcagaag atttaggtgt tgaaatggtt
      481 tggtttaatg atactcaaaa tccagtagaa ataataactg aacatataga caaaaatgct
      541 actatgggtg ttgataaaaa ctggccagct agattcttat taaacttaat agaattaggt
      601 ggaggaagta agtttgtaaa ttcatcttat ataatagata ctttaagaat gtgtaaagat
      661 gaagaagaaa aagagttaat gagaatagct tcaaaattaa atgacaaggc tatggaacaa
      721 cttaaagcta ctgtttctgg tgaacttaca gaaaaacaac ttgttggtaa attatctaaa
      781 atatatgaag acctaggtac tgatggattt tcatttgacc ctattatagg ttttggacct
      841 aatggagcta atccacatgg tgaacctgga aatgcacttg tgaaaccagg agatgcaata
      901 atcttagata taggttgtat aaaagataac tattgtgctg atatgacaag aactgtattc
      961 tataaagaaa tacctgaaaa aggtagagaa atatttgaaa tagttcttga agctaataag
     1021 agagctgaag ctattgtaaa accaggtgtt agattctgtg atatagatgc tgctgctcgt
     1081 gactacataa ctgaaaaggg atatggacag tacttcactc atagaactgg tcactctata
     1141 ggacttgaag ttcatgataa aggagatgta tcttctataa atacagatac tgtacaacca
     1201 ggaatgatat tctctataga gccaggtata tatttaccag gtgagttcgg agttagaata
     1261 gaagatttag tacttgttac tgaagatggt tgtgagatat taaacaagca tgacaaagaa
     1321 atatgtgtag ttggttaatt ctaaaatcaa tttgataaaa ttaaaaagcc atataaaaat
     1381 gttttaaaca tttttatatg gctttaattt ctcaatctat tatttatttc aatccattat
     1441 ttatattgca gtatttatat tgcagttatt aagattgcac cctttgaagt atatgtcccc
     1501 atcactgaac caatttcaca gattacaaca tctttgtatt ttacatcctt ttccataact
     1561 tcccttacct tttgagcctt ttctgggcaa ttagcatgtc ctataaataa aaccttttct
     1621 tctttatctt taatttttga ctctactaaa gtaatcagct ttttcaaact gtttccttca
     1681 cctcttgcct tatctatagg agttacaacg ccatctgcaa cttgtattat cgccttaaaa
     1741 tttaaggtat ttataatttt tcctgctatt ggatttattc taccaccctt aatagcattt
     1801 tctaaagttt caagtgttcc aaaaaatgga ggattatctt tatatttatt tattattgaa
     1861 acaatctctt caatttcttt tccttctttt attaatattg ctgcttttaa cactaattgt
     1921 cctattccaa ttgacccact aagtgaatct ataacctcaa ctttcttgtt atttccttcc
     1981 ttttcaaaca tatcttttgc taaaagtgca ctattataag tcccagataa tcctgaactt
     2041 atattaatca ctaatacact ttcttcctga caatggtatg cttctaaaaa tttatcagga
     2101 gaaggacatg atgtttttgg tagtacttta ctctcactca tttttctata gaaagtttca
     2161 ttatctattt cctttccaga tatataactt tcctctccaa aagatacatt taaaccaaca
     2221 acttctatgt tgtatttttc tattatatca tcaggtaagt cacatgcact gtccactatc
     2281 aattttattt tatccatatt aacctcacta taagatatta ttttatttat tcagtcggtc
     2341 gtacgaccga tttaattatt attttatata ttgaatgaaa aaaagtcaat agatatagat
     2401 tctcgcttta ttgacaataa atatccataa gcttataatg ttattaattt gttataaata
     2461 tacatggaaa ggtaggtttt atgaaaacaa ataatatgaa acaggctatt ttatcatctg
     2521 caaccaattt aatagccaaa aatggagttc aaaatactag ccttgctgat attgcaaaag
     2581 atgtaaatat aagtaaaggt actttgtatt atcactacgc atctaaagat gatattatat
     2641 atgatattgc ggatactcat ttagaagtta taactagtgc tatactaaac tgtgtaaaaa
     2701 atgtcaaatc aaaaaattct caaatagaaa tggttaactt aatcttagag aaaatatcta
     2761 ctatagaaag tcgtggtagg gtacatatgt atcttatttg tgaggcaatt acttcaaata
     2821 atgatttaaa ggaaagaata agattaaaat atattgaatg gagaactact cttaaatctg
     2881 aaattgttga atcactagaa aaatataata gtagcgaaaa tgaacaggat gctgaatcat
     2941 tttctttttt actcatgtca atagttgatg gtttagttgt ccaaagttta ttaaaaacag
     3001 aaaaaatacc ttatgaaaat atagcttctt ttttagttaa tcattggatt taaacttaat
     3061 acttagataa ttaataaaag tgaactattt aagttatcta ctttaacagt tcactttctt
     3121 atttattctc aataattttt acttaaaaca ttcaattttt atttaaacct tcatggaatc
     3181 acaaaataaa aatctcttat aaatttaaaa tgtttttatt tatgttcatg tgtatgccca
     3241 tctgaatgtt catgaccatg tgtatgtgta tgctcatggc tatgctcatg agtacctgaa
     3301 aaaccatcat catgattatg gtcgtgacct acaccattta catgctcatg ttcatgccca
     3361 tgactgtgag aatgttgatg tgaatgttca tggttatctc ctccacagca tccttctcct
     3421 ccagtcccac aacacccact tgtatgtgga ttatgatgat ttcttactac aaatctctct
     3481 cttaacataa ttcttatacc tcctagtata ttattgagta cttcatgtac cccattaact
     3541 acagtataca tctaactagg tataatctca atacatatgg cattttttaa catatataat
     3601 taacatatat aatcttaaag aaatttatcc ttaaaacctc caccttttac ttcttcaacg
     3661 ttattgacag aaataaaagc ttcactatcc atatcataaa ttaaatcttt aaatttacct
     3721 atttcatttg cagatattat gcaataaatc atctttttct tattttgtgt atatgcacct
     3781 tctgcctcca aaaaagtgac tcctctaccc attttattca ttatcacttt ggatatttca
     3841 tcatatttat ttgatattaa aagtatagat ttttttctat caaagcaatc ttttcctata
     3901 tccatagtaa aagaaattat atacattgta attaaagtgt acatcaccaa tttagctcca
     3961 aataaaaaag ctcctgcaca tactacaaaa aaattaataa ataaaaatgt atttttcata
     4021 ggtatatcat actttctttt cattatggct gctattatat caagtcctcc agcagtagct
     4081 cttgctttaa aagttaaccc catacctata ccagttaatg caccaccata aatacactgt
     4141 agcatagtat catctattgg gaaatattta gcaatatctt gtgttagccc cagtgctaga
     4201 gaaaatagta acatattgac aaaacttatt agcaaaaatt tattatcaaa atactttcta
     4261 gctataataa atattggtat gttcatcaag aaacttgata ttccctgatt tataccaaat
     4321 aatttgtata atataacaca aattccagct actcctccac ttaataactt ggctggtgct
     4381 agatataaat ttattcctaa ggttgataga atcataccca atataataag acaatattgt
     4441 aaaaatatat tattatcaat acttagcttt ttactttcac catacatcaa aacgcacccc
     4501 tttgcataat aaataaacca ccctgcataa cacacgataa atttacaaat aaatttatca
     4561 gaagtagttt cctaaaaata ttattcatcc tcacttaaac gtttattcat taatattcta
     4621 tcacatgaca taaataattt atatgattta tatagattta tttattgtaa attttggaat
     4681 attaaaacat tttattgaat agataattat attacataaa aaatccctat agaaatctat
     4741 aaactaattt ctatagggat tttggttaac taattattat ttttattttt ttataacttt
     4801 tatacctgtc atatggtcat ttaagttttg ttcctctaaa gatttatcat ctactctgtt
     4861 tatttctaaa gctaaagttt cagacttgat atattcatta aaatggtcaa cagccttagc
     4921 aatttcatca tcaccacagt attctattac tatattatct aatacttcaa agttattaga
     4981 ttttcttaat tgctgtactt tagatataaa ttctctagca taaccttcat taatcaactc
     5041 ttcagttagc ttagtatcta gtataacaaa aagattgttt tcaacagata cattaaatcc
     5101 ttccttagca gttataccta ttaatactaa atccttatta aattcaaaag cttcaccatc
     5161 taaatcaact gttaaagtct cacctttttc aagtttatta gcagtttcat gagcatttaa
     5221 tttacttaat gctccaacga ataaattcat cttagaacct aataatggcc ctgcttcctt
     5281 aaagtttggt tttaaactaa agttcatata ctcatctaaa tccttagcaa atattacctc
     5341 tttaacatta agctcttctt ttattaaatc aactacatca cttattgtat cttcaaactt
     5401 accatctact aacacttttt gtaatggttg acgaactttt attctttcta cttctcttga
     5461 agctctacct aaagtaacta agtttttagc taaatccatt ttttcttcta atttagtatc
     5521 tactaactct tcattacatt taggatattc agctaaatga acagatactt ctcctgttaa
     5581 gtttctatac atttcttctg acatatatgg tgcaaatgga gctatagctc tacaaagacc
     5641 atgtaacaat tcataagttg tattgtacac tgctttttta tcttctgtta attcagttgc
     5701 ccagaatctt cttcttgaac gtcttatata ccagttagat aaatcatcat ttataaaatc
     5761 ttgtatcatt cttaaagttt tatttaactc aaatatagct aaattttctt ctacatcttt
     5821 cattaaatta ttgaacttag ataatatcca tctatcaagt tctggtctat ctttatattc
     5881 tacaaaaaat tcagttggat ttatatcatc tgtgtttgca tataaagtaa agaagttata
     5941 tacattcttc atagtaccta taaatttact ttggatttct tttaatccat ccatatcaaa
     6001 tttagttgga gtccatggtg gagaaacata tagtaaatac catcttaatg catctgctcc
     6061 atattggtca aataattcca ttggatttac tgtatttcct cttgatttac tcattttctt
     6121 accatcttta tcaagtacaa ggtctggaac taaaacactc ttatatggtg cttttcctaa
     6181 tacaaatgtt gatactgcaa gtaatgagta gaaccaacct ctagtctggt ctataccttc
     6241 tgatatatag tcagctggga acaattggtc aaagttttcc ttattttcaa atggatagtg
     6301 gtgttgagca aatggcatag ccccactatc aaaccaacag tctataacct ctgttactct
     6361 tgtcattggt tttccacatt tttcacattt taagtgtata tcatctacat atggtctatg
     6421 aagttcaact gtttctggat ttacatcttc tatagctttt tcagctaatt cagcccttga
     6481 accaactgaa tctgtatgac cacattcaca tctccatata ggaagtggag ttccccaata
     6541 tctagttctt gatattgccc agtcatttag attttctaac cagtttccaa atctcttctc
     6601 tcctacaaaa cttgggaacc attttacacc attattattt tctattaatt tatctttaag
     6661 tttagtcatc tctatatacc agcttggttt tgcatagtat actaatggag tttgacatct
     6721 ccagcagtgt ggataattat gaactacttt ttctttgcta tataatttat tttcagaagc
     6781 tagccatttt attatttcaa catctagccc atcttccatt acaaatctac cttcccaagg
     6841 agtagatgta aatttaccag tttcatctac tggttgaata actggtaaat catatctttt
     6901 acctgtgttg tagtcatctt caccaaatgc tggtgctgta tgaactatac cagttccatc
     6961 ttcagtagtt acataatctc ctactgtaat aaaaaatgct tttttgtcaa cttctacaaa
     7021 aggcattaat tgttcatatt ctaaaccttc taggtcttta cctttcattt cttctaaaac
     7081 ttcataatct tcacctagta ctttatcagc taaaggttta gatacataat atacctcatc
     7141 acctttttta acttttaaat aatctacatc agcatttact gttaatgaaa catttgatgg
     7201 taaagtccat ggagttgttg tccatgctaa gaagtattca tcagcatctt ttttcttaaa
     7261 tttagctata actgtattag tttttacttc tttatatcct tgtgcaacct catgactagc
     7321 aagaccagtt ccacatcttg ggcaataagg aagtatttta tgtccttcat atatatatcc
     7381 ttctttgttg aatttgttta gtatccacca tacagactct atatagttat tgtctaaagt
     7441 tatatatgga ttatctaaat caacttcata agccattctc tcagtcatta ttctccattg
     7501 tttttcaaat gagaaaactg actctctaca tttttggtta aattcagcta ttccatattc
     7561 ttctatttgt tgcttgctag taagacctaa ctctttctct acttgtattt caactggtaa
     7621 tccatgtgta tcccaaccag cttttctctt tacttgataa ccagacattg ttttatatct
     7681 acaaactgaa tctttaagcg ttctagataa aacatggtgt actccaggat ttccattagc
     7741 tgttggaggt ccttcataga atacgaaact tgggtcatct ttcccttttt ctaaagtttt
     7801 ttcaagtata tttatgtctt tccaataatc aaaaacttgt gcttctgctt gttttacaga
     7861 actgtctacc aatggcttaa atttcgccat gtaaatcacc ctttcctact aaatattata
     7921 attccaaaat tttttattct ttcacataaa tttaaatact aaaaaactcg ccccctttaa
     7981 accaaaggga cgagttataa atcgcgttac caccctaatt ctatatgtaa actaaatact
     8041 agctacatat aacactctaa tagtataacg gtactaaccg gcacagctta gtagaattta
     8101 atcgttcagc ctgcagctca agagtgattt ttatctttat aaattcattg gttctcagca
     8161 tacccaattc tctgtaaaat tatctaaaga taaatgtctc tatcaacgct tttaaaaatt
     8221 tatctgtgtt tatatgaaat tctataacaa ataatttgaa tagtcaatat atttttgaaa
     8281 aatataataa tttctatttt tcttacattt ttatcctatt tttaatcttt gttatagatt
     8341 tctcaaactc acacaattat tctacataaa aaataaacaa attacaagct tattctaaag
     8401 ttgtagcagc ttcatcatta ctataattat acactgttgt tccttcaaat gcgtttacac
     8461 atttttcatc tacatcataa aaaatctcat caacacttct tatttcatct tctaacagtg
     8521 atttaaattt atttctaaat atcttaaatt ctttgactaa agcatcatac tcttttctta
     8581 tctctataac acggttatta gcttgttcta taatttgtct agcttttaaa tcagcttctt
     8641 caacaattat cttagccttt ttattagccg cgctacaagt gtcttctgct gcactttgag
     8701 cagttattaa agtggctttt agtgtttctt caatattttc atacttgttt acttggtctt
     8761 gatataaccc taatttttct ttaagagctg tattttccct acatagagat tcataatctt
     8821 ccttgatgat atctaaaaat tcatcaacct cttcatctct ataccctctt aaaccttttt
     8881 taaactcttt attttctatc tcaattggag ttagcattac atttcctccc agctataatt
     8941 taaattagtc cttatacaat taatcttgct tgaactttta tttttccttt tttagtaata
     9001 tctcctattt gtgttacctt agctcttcct ttgcctctaa tagaaatcaa atcatcgttt
     9061 tttactattt tagaagttga agttatcttt tcataattga catggacttt ttctccattt
     9121 atgtattttg cactatcttg tctagatata ttgtaaatac cacttatcac acaatctaac
     9181 ctatctgaag atactgtaaa tgatacttct ttatattttg aagaactatt aacaatattt
     9241 tctctggaaa tttcctttac aattacatta tttcttgaaa ccttttctaa gtttacaatt
     9301 ataaaatcgc aaatatcact acttacaact acttgacaaa agctatcatg tataattata
     9361 tctccaattt tttctctttt tatacctaga cccagtattg accctaagta atctttatga
     9421 gatatatttt taaatttgca attcccttct atttgtaaaa atctaagagt atcttctata
     9481 tcttcatatt ccatgtaaaa tggataaata aatacagtgc ttctttcagc ttgttcataa
     9541 cctccatcca cactgtattt tatttcattt gtagaattta atattgctac agcatttttc
     9601 acttcatatg gattaagaaa ctccgtagac ttaacatcat agttttttat acaactatta
     9661 gccttgtcta tcactttaaa cattttattt ttcaagtcta tatcctttat gtgatttgtt
     9721 agttttaatt tatccatcct atcaccttag aatataacca atactaattg tcttaggaac
     9781 atcaataata ctattgcaat taaaggagaa aaatctatag gtccatttgt atatttatac
     9841 attatagacc ttattggccc ttctattggc tctgtaagat tttccaaaat atcaaaaatt
     9901 ttactttgtc ttccatttgg aaaccaagtc atcaagcttt ttataactat catccatgct
     9961 attatatcta aaagataata aagtgcaatt cttattgttc ccatatatta accgcctttt
    10021 tactatttat tttgccaagg gaagaatgct ttactttcta attctttttt catatttgca
    10081 tctatatcca cattattagg agctagtata aatattgatt tagaaacctt ttgtatactt
    10141 ccatctaaga cataaactgc cccattcata aagttaaata tagatttacg aacttcagaa
    10201 ttagtcaatc cttccaaatt cactataact gcccttcttt gttttagatg gtctgctata
    10261 gttgcagctt catcataaac cttaggttca actattacaa ctttcatttg gctagttgta
    10321 tgtaaattta caattttatt tgcttttgca gtactaaagc cactgttcat ttcttcttct
    10381 tgaactatat cgtccatgcc actttcatat tcatcttcaa tgtaatcttc ttcttcatca
    10441 actatccaat tcttaaattt agatattatt ccatttgaca ttattaactt cctccttaaa
    10501 atttattcaa atttgagtaa ttatttttta ttgtagtttc tttctccaaa gattgatgtt
    10561 ccaactctaa caatggtagc cccttcctct atagcaattt cataatcgtg actcattccc
    10621 attgacaaaa catccatccc tacattaggg atgtttagtt cttttatttg ttttgataaa
    10681 tctttaagac ctttaaatac acctcttatc tcatcttcat cttctataaa aggtgccatt
    10741 gtcattagac cctttatttt tatattttta tatttttctg acacttcttt gacaaagtct
    10801 acaacttctt ctctatcaag tccatgctta gactcttctt tagatatatt cacttgtact
    10861 aagcaatcta tttctctgtt tatttgttct gcacgttttt gaatttcttc gcataatgca
    10921 attctatcta aagagtgtat catacaaact ttatcgatta tgtacttaac tttattggtt
    10981 tgcaaagttc ctatcaggtg ccagtttaat ttatcaccta taatatcata ctttctagct
    11041 aattcttggg gtttattttc accaacagaa gtgattcctt ccctcattgc ctcattgact
    11101 aagtctacat caacagtctt tgtgactgcc aaaagggtaa tctctttgct gtctctatct
    11161 actttttttg cagctttatc aattttgttt cttatctcag acaaattatc tttaatagaa
    11221 cccattatta tcacctactt tatccttata tttgtgttta tactgttagg tcttaaaatt
    11281 attttgtcat atagattaac acttgtaact ccatttatgt catttttata ataatccaca
    11341 aatacagaag attcattctc ataagttatt ccttctaata tgacaaattt tacactttgt
    11401 gattgttcat caattacata tacaccacgt ttattgtctt ttgtttttat agaagtttta
    11461 ggtattttta aacattccat ctgattatat attatatcaa attcttcgac acttgtatcc
    11521 tgatttgcaa caatttggtt acttattttt aattttacca ttattttatt attattttca
    11581 tatattttgt aaacttttgc atctaatttt tgctcatgat attcaattat tacattttga
    11641 tttttttcaa agttattaga atcttcttta ctgatgtatg tagatagata attactctgg
    11701 tcattggcat ctttttctat acttcctttt acatgaatct ttgcatcaac tttttcattt
    11761 tttaacacca acgtatttac atttttgcta attatcatta ctaatatatt aaatactaaa
    11821 tatataagaa taccaagtat cactaatctg gtatatttta cctttttcaa agactctccc
    11881 cctataaaat tactttcatt ataagtactt cttctaaaaa atgtttattc cttttttata
    11941 agctaatatt ttacgttcta ataattattt tttccatatt aaatttattt tttatcaatc
    12001 ttcctataca agactgcatt atttatatat aattgtatta taaagtataa gtttaattta
    12061 cattttatac gttaagctta ctttatatgt ttttatagtt atttttattt ttagatacaa
    12121 tataaaacca attaagattt aaaataactt ttgttatgtt gacatttatt taggtaatca
    12181 tttacgaatc aggataacgt tatcataaca aaagtttatg tcctaaaaaa aatacaagga
    12241 ggattttctt atgtcagaaa ttttattagg aacaggttta cttttattgg ttctatgttg
    12301 tttcacaatt tttagttaca aggccccata tggtatgaag gctatgggag ctctagcaaa
    12361 cgctgcatgt gctactttct tagtagaggc attcaacctt tctttacttg gtgatgtttt
    12421 aggtatggaa ttttttagaa gtgttggagc tgctaatggt agtcttggtg gagttgctgc
    12481 tgctatactt gtgcctttag ccttaggtgt atctccagtt tatgcagttt taataggatt
    12541 atcttgttta gatttttcta tattatctgg attcgttgct ggatactttg tttctttctt
    12601 aattaagttt atagagaaaa aagtgccacc tggagtggat ttagtggttg taattcttct
    12661 atgtgcacca ctatctcgtc ttatagcatc aaatgtagac ccattagtta atagtacact
    12721 actacaaatt ggacaagttt tgatagaagc taccaacatt agccctataa taatgggaat
    12781 tatactagga ggtctaataa cagtagttgg aacgtctcca cttagttcaa tggctcttac
    12841 atctataatt ggtcttactg gtcttcctat ggcaatagga gcattaggtg cttttggttc
    12901 agcttttatg aactcaacac tatttgcaaa attgaaatta ggttctaaaa aagatattat
    12961 atctgttgct attgaaccat taacccaatc agacataatt tcagctaatc caatacctat
    13021 atattctact aatttctttg gaggagcctt ttctggtatc attgtagcct tgatgggtct
    13081 tgtaaacatg acaccaggta cagctacacc tgtggctggt tttgctataa tgtttgctta
    13141 taatccagtt caacaagttc ttataacagc tggtttatgt gctttattca atgtaattgc
    13201 aggattttta ggttcaaaaa tattcagtac ctttaaagtt cttactgctg gtgaaatacg
    13261 tggtactgat gtagaagaag aaattgctta aaaaacaata agatttataa atttatatct
    13321 caagtagtag agttttatat tgtaggcaat tttattaatt gcaaacgttt tattttttat
    13381 ttatgtttat gattattatg tgtgttgaat atttagataa atttttattt attattttta
    13441 tttagatttt tgtttagttt tttatttggt tgatatatta ggaataaaaa ttagtatagt
    13501 ttattactag ttagatggca taacttttta gatacataaa aaattttata ttaattagat
    13561 attattaaaa taaaagcgat acatgaattt ttaaatgtcc atgtatcgct ttatttttgc
    13621 cataatctta ataactgatt tttaaagttt attatcttaa caaagttcat tatcttatat
    13681 aatatttaaa tgcataggct tctttaattt aaccatctaa atatttaatt aaacattatt
    13741 atatgtaata taactaattt ttaaatgaat gtataggagc tggaatcctt cctcctcttt
    13801 gagtaaataa ttcagatgaa tttgtattta taggcattac tggtgctcgt cctaaaagtc
    13861 ctccaaattc taccatatca cctacatcac atcctggggc tggtataatt ctaacggctg
    13921 tagtcttatt attaatcata cctattgctg cctcatcagc tatcatggct ccaagcgtac
    13981 ctgctgtagt atttcctggc acagctatca tatcaagacc tacagaacaa atagcagtca
    14041 ttgcttcaag cttatctata cttaaagcac ctttaataac agcatcaatc atacctgcat
    14101 cttcagatac tggtataaac gctccactaa gtcctccaac atgtgaacat gccattactc
    14161 cacctttttt aacagcatca tttaaaagtg ctaatgctgc tgtagtccca tgagtaccaa
    14221 caacttcaag acccatttcc tctaatatat ttgctaccga atcaccaata gcaggtgtag
    14281 gagctagtga taagtctatt atcccaaatg gaacatccaa tctactagaa gcttcctttg
    14341 ccacaagttg tccaactctt gtaattttaa atgcagtctt tttaactgtt tctgaaacaa
    14401 catcaaaagg ctctcccttt actttctcta aagctctctt aacaacacca gggccactta
    14461 caccaacatt tataattcta tcagcctcac caacaccatg gaaagctcct gccataaatg
    14521 gattatcttc tactgcatta caaaatacta ctaattttgc acaaccaaaa ccttttgcat
    14581 cttttgttaa ttctgcagtt tcttttatga tttctcccat ttctttcact gcatccatat
    14641 ttataccaca tcttgtagaa cctacattta ctgaagcaca tactttatta gtacttgcta
    14701 gagcttttgg aattgactta attagtatct tatcaccttt tgtatatccc ttttgaacta
    14761 atgctgagaa tccaccaata aaatctatac ctaaatcctc tgcagcctta tccaatgtct
    14821 gtgcaaactt tacatagtca ttttcatctg ttgcacctgc aataatggaa attggagtta
    14881 ctgataccct tttatttaca attgggatac caaattcacg ctctatatct tttccaacct
    14941 tcaccaaatc tttagcagaa ctcataatct tattgtatat tttttctcta gctttatctc
    15001 catcagggtc tatacaatct aataaagaaa tccccatagt tatagttctt atatccaact
    15061 gttcttcatc tatcatcttt attgtttcaa gtatatttcc tacattcact attatcacct
    15121 ccccttactg tctactacag attatgcatt gaattaaata tttcttctcg ctgaattttt
    15181 acatccactg ataatttctt acctctttca accaagtcct caaccgtttt ctcaaaagaa
    15241 tttaatgatt tctccaaatc aactatcata atcatagtaa aataatcctg taatattttt
    15301 tgtgatatat ctactatgtt gatattttgc ttgtataatt catcactaat tcctgccact
    15361 atacctggtt tatcctttcc aattactgtt aaaattgctt tcataataat atcctccctt
    15421 atttttttat aaatcttata tttttacaaa ttgaactatg tgttaacata tatttcattt
    15481 atttgttagt aaaccacttt ttatgtatat atttaatata ttttaatacc tatacattca
    15541 tttgtcaatt ttttttcaat attctattat ttttatacta tttagaaaat tcatatatta
    15601 tatatgtttt ctcaacaata aatacagttt tcataataat gaaaggtatt acttagcaat
    15661 ctcatgattt tctaaataat acctttctaa aaattatttt tttaatttta tgaatttaat
    15721 tatacttttt ctagtatcag gatttacaat taatatagaa ccaatcataa ttggtgttag
    15781 tatacaaaat aaaaaaataa tatctttttt tagattatat tctttagatt ctaatttata
    15841 attattattt attattccat cataatcact tatatttgac ttatttttat ttgtatcttg
    15901 atttttgtta gaaatacttt ccctataagg aaaagaatgt tcattggtac caaatactga
    15961 ttttgagctt aaatcataat tatcatcatt ttctgtaatt gaatttttat tgtaaaatcc
    16021 tttttcttta tcagaattat tttctttatt accgtcttta ggtatatcat gaggattttt
    16081 attttcattg ctatcagtac ttgattctac atcatcttta ggagtatttg gttgtttctc
    16141 atcagaatta tcattttcat tattattatt ttcattatta ttgttttgat tgttattact
    16201 atcattattt tcatttccat tattcccatc ttgattgtta tcatcctcat ctctatccgg
    16261 gtcgtctcta cctcctcctc cacggtcacc atcaatatca tcattttccc gttcatcatc
    16321 tggtgtctct tggttgtctc cgcctcccat tataaatcct ggtttaaaaa ctatatttga
    16381 atacccttct tttaatcctc ctaccacttt tactctaaaa caacgcaact taaaatcttc
    16441 aaaaaaaagt atcgcttgat ctctaatatc ctcctgttct tcagatatcc aattgattcc
    16501 atcgcttgaa tattcaacaa aaaccttact tgcactatac ggcatatcat aaaataaaac
    16561 agctacatat ccattttttg tattttgaaa ttccagttcc aaattgtcta ttggtttttt
    16621 ctctataaca gatacattca aactaggaac tacatcatca tttattataa ttttttcttt
    16681 atttaacatt tctgttttaa actttcctgt tatattaaaa tcttctctct tttccaaatt
    16741 ttcataataa tcagtaaaat cccattctac atctatatca agtcgattac cactagatgt
    16801 ttctaatacc atatatttgg gaaattttat attttctatt ttatcatctt tacttacttt
    16861 tatttcatca ctaaatacac caattcccct atgacaacat ttcacaatat tgtagccaga
    16921 atcttcagct atagagggta taagctcatt ataatcatca attatataaa ctttcttact
    16981 ttctgatata attgattgtg ccaacttatt attatctctt agtaacccac tttcattgtt
    17041 gctactatat gcttttattg aagtttcttc tatctcaaca tcacctttag aataaacacc
    17101 tatagcatgt tctccattca cttcaatatc cttatttagt attctaatat cattttcagt
    17161 ataaattcct attgcatctt tcccatttgc ctttattttt tcaacagatt cgtaggatga
    17221 tttaatatgt aagtctcctc cttcttcaac gtataaggct gtcccatcag tagcaattat
    17281 gttgtttata ccaatactta tacttccacc attttctata tgtattactc cttcttttcc
    17341 tccttcacct attatattca attcattatc atcaataata aatctcccat gattttttat
    17401 caatatttta tatttgttgg tatctattgt aaaattactg tcataaggaa tataaaatct
    17461 aaactcttca tctattacca tatcacaaga aagagtataa actatttctt ctttattatt
    17521 tttattttct tgaacccacc tttcaaaatc ttcaagacaa ttttcctctt gtccaaaaac
    17581 tttatgtgga aaacttataa aacacaaaac tattatactc ataaaaataa atatctcata
    17641 ctttttcatc ttacactcct agaaattttt agctttgaaa caatcctgtc gaaacatata
    17701 cttcacccac tataattttt ccacaattat caattattgt caatttatat aagtatgttt
    17761 attaattgtt ataaataaaa agctacttac taaagcaaac agacgtttca ctttaataag
    17821 tagctatatt tttataacaa aatagttata atattagaac ttccttcatt tacaattttt
    17881 tgtaaagtct tttgcatttt aactcttatc tcttctggca tagtatataa tttactttgt
    17941 aattgctctt ttactaagtc atgtagtgac ttaccaaaca tatttgattc ccataaatct
    18001 gctggttgct cttcaaatac ttccataaga tattttatca tctcttcaac ttgattttga
    18061 tttccaacta ttggagaaac ctctgtagat atatctgctt ttataatatg aagtgatggt
    18121 gcattggctt ttaacttgat accatattgt tttccttgtt tcattatttc tggttcttcc
    18181 agactaagtt cttctaagga aggagctact actccatatc cttttatctt ggcatctatt
    18241 aaagcacttt ctattttatc atactcattt ttaactttag atagtctagt tatcagacta
    18301 agcagttgat aatctccttc aattttaaat ccactttttt cttcaaggac attatagaat
    18361 aaatcttgct ttgtctgtaa atcaatattt ataacacctt ctcctagctc tacattatca
    18421 acacctgtat cttccaaaaa ctctagctca gaaaatcctt gtataatacc ttctatatcc
    18481 cttattttac ctatatctat aatactttgt tttaatgttg ttattatgct actttttatc
    18541 caatgatttc tttcaagacc ttcaacccat tttggtaagt tgattcttat ttctgtaagt
    18601 ggaaaatcat ataatacagc ttccataacc tcctctatat cttcttcttc catttcaacc
    18661 acattcattg gaagaactgg aacttcatac ttttcttcca attcacttct aagcatactc
    18721 gtttcctcac tttttggact taacgtattt aaaaccactg caaatggttt tttaagattt
    18781 ttcaattctt gtataactct ctcttctggt tctacatagc tttttctgtc aattccagta
    18841 actgaaccat ctgtcaagac aactatccct attgtagaat ggtcttttat aactttttta
    18901 gttcctatct ctgctgcctt ttcaaatgtc atagcttctt gtgaccaagg agtggatact
    18961 aatctttgtt ttccaccttc ctcatgccct aaagctcctt caactatgta tccaacacaa
    19021 tctaccattc ttacttttaa agatactgtg tcttttattt ttatttctac accatctgct
    19081 ggtacaaatt ttggttctac tgtcattatc gttttacctg aaccactttg gggtatctca
    19141 tctcttgttc tatccttttt aaattcattg tctatattag gtataaccaa cttttccata
    19201 aattttctta taaaagttga ttttcctgtt cttacaggtc caactactcc aatatatata
    19261 tccccctgag tccttttgga tatatcttcg tatatgttat tattcatatt attcctccta
    19321 tacatctaat cttattaaat tatatttaga gttaagggtt aatattctta aaatagaaaa
    19381 ataaagacta gcaatattaa tttcccctat atacaaaaac tagtaaacta caatgctaat
    19441 tgaaattatt aaaatccatt acattaaaag acataattat agtcatgata attacaaatt
    19501 atatataaaa taatttatta tatcgtatat acatagacta tgctaagtaa aattaaaata
    19561 gccctcttta tattaaagag ggctaaaaca aatctattat ttttttattt tattatacaa
    19621 tagtttatat atcatcacct aaaactactt cttccatttc atgcttttta cttctcatca
    19681 ttagttctat tccaacttct ttaggatttg aacctttctt tattacagaa tatatagcat
    19741 tagttatagg catatctata tctaattttt cagcaacatc atgtgctact tcagtagcag
    19801 ttatgccttc tacaaccatc tttacttctt ttaaagtatc ttctaaactc atccctttac
    19861 ctatgagtat accagctctt ctatttctac tatgcatact tgtacaagta actattaaat
    19921 ctccaatacc tgaaagacct gcaaaggtag acatattagc tcccatagca attccaagtc
    19981 tgctcatttc actaatacct cttgtcataa gggcagcttt tgcattatca ccatacccta
    20041 atccgtcaca tatcccagct ccaaatgcta taatattttt taatgctcct ccaagttcta
    20101 ctccaactat gtctggattc gtataaactc taagtttagg gctcataaat aaatcttgta
    20161 tcatctgtgc tatttttaaa tctttagaag caactactac ggtagtagga atatcccttg
    20221 ctacttcttc agcatgagat ggcccagata gtattacata tggattttgt ggtaattctt
    20281 cttcacagac ttgtgataat ctaagcccag tccctttttc aagaccttta gctacattaa
    20341 caataacttg tccttctttt ataaatggtt ttattttttg gcaagtactt cttacagcct
    20401 gtgatggaac tgctaaaact attaccttac aatcttttat agttttttct atatcagtac
    20461 ttatagttat attatttgga aataatactc ctggtaaata gtctgtattt tctttagttc
    20521 tgtttatttt tttagcttgt tcctcttttc gagtccacat gctcacatca ttaccttttt
    20581 ttgcaagccc caaagctaat gcacttcccc agcttccagt acctaataca catactttct
    20641 ccatgtttaa caccacctga gtataatata tttactattc tttcttttga ccaatttttc
    20701 tctctgtccc atttaataat cgctttatat ttgctctgtg attatatatt actgatgcag
    20761 ttaagaacag agctactatt aaaccagctt tattttttac cataatcata aatattggtg
    20821 aacatcctat tcctacaaca gaacccaatg aaacatactt tgttatagct accaccaata
    20881 taaatactgc tagacacatt agtgttatta ctggatttac agctagcatt gcccctagtg
    20941 aagttgcaac acctttgcct cctctaaatc ctaaaaccgc tggccaatta tgacctgcta
    21001 caacacatat aactgccaga tatccagcta aaagagtatc tattccaacc aatcttgctg
    21061 caaattctga tattaaaaca gccattacac ctttaagaac atcacctaaa aatgtcattg
    21121 ctcctgctct ttttccaagt gtcctaagta catttgtcga acctgcatta ccagaacctt
    21181 gagtccttat atcaacccca gcaattcgct ttgcaactat gtatgaggta gatatatttc
    21241 ctaaaagata tgcaactaca gcaataatta tataactaaa tatctccact gaatttcccc
    21301 cctttagtta tattttaaga tatttatttt ttattttttt gtctatactc aaactttatg
    21361 gaagttcctt caaatccaaa attttctctt atcttattct caaggtatct ttgatatgag
    21421 aagtgagtta aatccttatc atttataaac aatgtaattt taggaggtct aatgtcagtt
    21481 tgagtcccat agtaaatttt taatcttcta cctttgtcag atggtggttg atttaacatt
    21541 acagcttctc caattacttc attcagtgct gaagttgata ttctcttaga atgttcattt
    21601 gacacatatt caactgtatc tagtatcttg ttcattcttt gattagtttt tgcagaaaca
    21661 aatacgattg gagcatacat cataaatgga aatttttctt ttatatcttt tgtgtaattg
    21721 ctcatagtct tattatcttt ctctattaaa tcccatttat tgattacaaa tatgcatcct
    21781 tttccttcat catgtgctat acctgcaacc tttgtatctt gttctgttac accttctaag
    21841 gcatctatta ctatcaatac tacatctgct ctatctacag cactcatagc tcttattaca
    21901 ctgtattttt ctattgtctc atatatttta ctttttcttc taagccctgc tgtatctatt
    21961 agaagaaatt tctgtccatt tttttcaaaa taagtatcaa ttgcgtctct agtagttcct
    22021 gcaattggac ttacaattac tctttcttct ccaagtatct tattgagtat agaacttttt
    22081 cctgcatttg gtttacctgt tatagctact cttattatat cttcttcata ctctgtatct
    22141 aatcctgctg gaaaattttc aactatctca tctaataaat ctccaaatcc catactattg
    22201 gctcctgaaa ctgcaaaagg cgttccaaat cccaactcat aaaaatcgta tatattatca
    22261 aattgacttt gactatcaat cttattaacc actaagatta ctggtttttt tgtctttcta
    22321 agcaattgtg caatttcttt atcagcagct gttattccag ctttaccatc aactataaaa
    22381 agtattacat gtgacatgtc cattgcaagc atggcttgat ttctcatttg agataatatt
    22441 atatcttcgc tatcaggttc aattcctcct gtatccacta atgtaaaata tttatctaac
    22501 cattctactt cagcaaatat tctatctctt gtaactccag gtgtattttc aactattgat
    22561 atcctttttc ctgcaaattt attaaatatt gtagacttac caacgtttgg tctcccaact
    22621 acagctacaa ctggcctact tatactcatt ttatttctcc ttccatcttt tgttttctat
    22681 tttaaaattt tatccacaaa atcttttcct tcattcaaac aagggataat ttttattctc
    22741 ataagttctt ctaattgatt taattcaata ttatctaaga aaatttcttc atctgctttt
    22801 agcatacttc ttgttatata aagagcttct cctaattctt tatcttcaag ttgttctttt
    22861 aaatccttag ctgttaaaag acctgataca gttattgtct ctccaaaaaa cttgtttttg
    22921 atttcatata catttacttg aagattttta aacttgtcca tcatagcatc tgccatactc
    22981 tgtataaatt catatgcaga atgaccagta gctattgata ctttcttctc tctttttaga
    23041 atattctcag gcaatatatt tagatactct tcaatctctg tttttaactt acgtatcata
    23101 cctacaccat cttcaaactg taaaaaacct tcatattctt catatccagg caattcacta
    23161 ttagatagta tataaaattc atctgataaa aatgcaaatc gagtacctaa tttttcaaga
    23221 tatttctgtt gtatttgatg tatttgctct atagttttac ttgcgctctt atcattaaat
    23281 atatttaatt ctactagatt ttctctatgc tttgtaatcc caactggaac tatcgcaaca
    23341 ctgtttacat atggataaag ttgagctagt tcttttattg ttctatccaa ttcctttcca
    23401 tcatttatac ctggacataa aacaatttga cagttcattt ctatatgagc atctgccaat
    23461 ctcttcatta taccatataa ttttcctgca aatttattgc ttatcatttt ttgtcttaac
    23521 tctggattag ttgtatgcac agatatattt ataggactta ttctatattt tataatatta
    23581 tttatatctt cttcactcat atttgtaagt gtgacaaaat ttccttgtaa aaaagataat
    23641 ctcgaatcat catctttaaa ataaagagtt tctctcatac cttctggcaa ttggtctata
    23701 aaacaaaaca tacacttatt tctacagctt ttagcttggt caattatcgg atttgtaaat
    23761 tcaacaccca aatcttcatc atagtctttt tctatctcgt atatgtagac ttcgccatct
    23821 tttttttgta tttctacatc taaatattca tcacttagta agaatctata ctctattata
    23881 tcatgtattg gctgttcatt cacagatata agtaaatctc ctacctctat acccatttct
    23941 tctgcaatac tatctttata aaccttactt ataatattat ttactttttt tacatttact
    24001 tccaatattt tcaccactct tttgtattca tcaatgctta ttataatgta ttttaatatg
    24061 caatttattt atttattata ttttatgata gcatttttaa gcatttcatg tcataataac
    24121 acattttaat tctaatagtc aatataagta aatagctata aaatagatta gaacaaataa
    24181 tattcaaatt taagtttctt aaaaatttaa atattatttg ttcttaaaaa aattttataa
    24241 atttaatgtt ttactatgat taacgtccca tttgtcatat catgaaccat tccacctaca
    24301 cactttgaca tcaacaatgc cttattttgt aagtctttct catcatttgc ataaaatatt
    24361 ggacaacctc caccattttt catattctta tcaagtgtaa ctatagctag ggtatattca
    24421 tttaaaccta tatccattcc catcttatgc acttcctttc tttcctattt tataattttt
    24481 tagtgataag ttttttcctt tagagcttga aagtattggg catgacttta cagcttccat
    24541 tactttttcc acattcttat ctactggtaa ataagcaatc attatacttt catcctcttt
    24601 atttcttcta ggtaatggta cgaatgaagg ttctccataa tctccatata aacctaacct
    24661 tgaatatata ttgtatacta ttgcttgtct ttgaccggag tcaaataata ttcctgcatt
    24721 agcataatcc ttatttttag gaattatttg tattcctaca cctctattaa tatatacttc
    24781 tctatcgctt tttagaccta tatttgttat accttttaaa tctcccacct ttagtataga
    24841 ttcgtccaca aaactaattt cagcaggcat tacatcagct atgtctccaa tactttttct
    24901 agtaagaagt ttttttaata taaatgctaa cactaatcca caaattgaac ttattaccaa
    24961 actcatccct tcacttattt tcgtttctgc aagtattaca taatacaatc ctattgttaa
    25021 aaaagatgta actatacaca tataatttct tacttcatag gttctagcta tctcttctat
    25081 gaatgcagct cctctcttta caagttgaac ttcctctaaa ttttgcaatg tatttcttct
    25141 atttgctctc acctgtctaa attgttctgc tgctagagat aaaaatgtta tggatgtata
    25201 agaacgattt ataagagctg gaaccaataa tgctcctaat gaagaagcga cgaatgctaa
    25261 tataagttga gatagtaaaa catttggttg cgttggatac tgtttttggt ctaaattcaa
    25321 cattataatt ctagatgtta taccaataat aacagcaaca aaaaatactc tatccattaa
    25381 atcactccta aaatatttaa tttgaaattt tacatgtaaa tctacaaagt ctttctagaa
    25441 aaatcactct gctttccttt tgatgtttct atgataggtg tacttttcac tacatctatc
    25501 atgacatcta tatctttgag tattggcata tatggtataa ccacagtact tttttctaaa
    25561 ttggtcttag atatagcaac aatatcaacc tcatctacat ctctatctat ccccatatga
    25621 ataaatagat tatgtattat agcttgtctt tgccctatat catttactat tccaaatgca
    25681 cctaaatctc ttggtataac ttctatagcc agacattcat ttctatactt ttctcttgta
    25741 ctttctagac ctatgttagt tattattaca ccatttacca ttaaaatagg tccttcaaaa
    25801 cttatttttg caggaactat atctgctata tctgctatac tgttccttct taaaaatctt
    25861 ctaaaaatca aaccaacaat agctcctgac acaatagcta agattgtgca ataaaaaaag
    25921 ctaaggccat actttcttgc aaaaacgata taaactatcg aagataccag tgcagaaaaa
    25981 agacttatat aacttctaga ttcatacgta gatgctattt cttcaacgta agcgtctcct
    26041 tttggtacta attcttcatt atcgatattc tttagtgtta ttctttcttg ttctgcaaga
    26101 ccttgaaact gttgtattgc tactgcaaaa aatgtcaagg ctgcaaactc cttatctata
    26161 agtgcaggta atgctatagc acctaacgaa gctgaaagcc cagatataat tatctgttct
    26221 aaataatctt gtggtcttga tgggtattgt ttgtctgtaa ctcttaaaac taatcctcta
    26281 cataatattc ctacaactaa tgcaactata aaagagtgtc taaataaatt tgcattcaat
    26341 aaactttctt ccattttctc agctccttct cttatatagt ttgtgcaaaa aacaaaaaaa
    26401 attcccttct atttaaggaa atttttttgt aatattgtaa ctataaattc gctaccaatg
    26461 ccaagttcac tttttacttt tatacatcca tttagtgatt tgactatatg ctttgttata
    26521 gccaatccta aacccgttcc tcctacatcc ctacttcttg ctttatctac cctataaaat
    26581 cgttcaaata tgcgttctac atcttctttt ggtatcccta caccattgtc tataacttta
    26641 attacgattt tttcttcatc ttttaacact acaacttgta caatcccatt atcttgggta
    26701 tactttatag cattatcaac taggtttaag aacacttgct ttatatagtc tttattggat
    26761 agtatactta ttgatttatc atcacattta tataaaactt ctatattttt ttcactagct
    26821 gaatgttgag ttatttcata tacctcttta aatacatcat atactacaac attttccata
    26881 agaagagtat ctttattttc tacaaatgat aacagcaata tatcatcaat caaccttttt
    26941 aatctatctg attcgctttc tattatttct aagaatctat ttctagtatt cacatctata
    27001 ttttcatttg actttagtgt ttcaacaaac ccacttatag atgtaagtgg tgttttaagt
    27061 tcatgactta catttgcaac aaaatcactt ctcatatttt caagtttaac ttttttagta
    27121 atatcttcta tatttactat cgaacctatt attacgttgt tagtattctg aagataaact
    27181 gggtctactt ttatcttata tacgatatca tctttagtag taatattagc agtttcatta
    27241 tctttagaac caataaattg aagtatttgt tttaatattt cttcttcttt tattacaaca
    27301 ttaatattat gaccttcaac taattgattt ggttcactct ttatcatttc ttttgcttca
    27361 tcatttatca gaagtatatt tccatttaca tctatagcta aaattccatg agatatactc
    27421 tttaatatag accttagctg taaatgttta tactgtactt cttctaaggt tttatccata
    27481 ttctttatca tatgattaaa atttctagct aattcgccaa gttctccttt ggctgatatg
    27541 ttaagcctag catgatattc attattactt actttttttg agacgtatat aaactctttt
    27601 aaatatttcc ttaatgttat tgtgtatctt acagctaata taacaatggc tatggacaac
    27661 ataacaataa aaaaatatat actatttaaa tcttccacaa gataacactc ctaaaatata
    27721 acctttttaa tctattttgt atcctacacc tcttatagtc tctatatatt tttccgattt
    27781 atcttcatct tctatttttt ttcttaaata tcttatatga acatctactg ttctagtttc
    27841 tccatagtat tcataccccc atattttatc caaaagataa tttcttgaaa gtacttttcc
    27901 tttattttga attaataatt ttaacaattc aaattcctta agagttaact ctatcttttc
    27961 agagcctttg gtaacttcat gttttgataa atctagtttt aaattacctg tagtaagtat
    28021 attgttttct tcacctaagc tagtcaaatt atatctcctt aaaacagaac taatcctagc
    28081 taacaactct tttatactaa atggcttagt tatatagtca tcagcaccta tttcaagccc
    28141 ttcaaccttg tcattttcca tatttttagc agtaagcatt ataacaggaa tattttttaa
    28201 gtccttatca cttcttattt ttctcaaaac ttctattcca cttatatttg gtaacatcca
    28261 atctaaaagt attaaatctg gtttaatttc cttagccttt ataaatccgt caaatccatc
    28321 atatgaataa ctaacttcgt aatttgacac ctccaaattg aattttaata gctcaactat
    28381 atgcatttca tcgtctataa caagcacttt agtattcatt aaaaagctcc tctctatttt
    28441 atctgtagta ccgttcctat tcgttctgat gccttattat gttttctata agagtggaat
    28501 ttatctgctc tacaactagt gcataaattt aaatttatta tattttcttt tttaactcca
    28561 ctacatctaa gcatatactc attaattttc cataaatcta aatagtaact tccctctttt
    28621 attatataga acttttcatc attatttgta agaattgtgt taaatttttc tactaaatct
    28681 tcagacactt cataacaaca tggtccaatt gatggcccaa ttatacatac taaatcttca
    28741 ggaactgttt tatagcattc agacattaat tttattgttt tatgacctat attactaaaa
    28801 gtccctctcc aacctgcatg agccagtcct atggctttat ttttagcatc tataattgat
    28861 ataggtacac agtctgctgt aaacagtaaa agtggtactt tttccaagtt tgttataagt
    28921 gcatctccat ctcttctttt ccctatagta tctttattga ctatattaac tatatctgaa
    28981 tgtatttgag aattactagt tagattttca aatgtcaaat cacattcttt acatactttt
    29041 aacatatctt ctctattttt tgcatctata ttcttacatg ttataactag atttacagaa
    29101 tttaattttt catcaagttc ttcacaatta ccacttaaat taacatactc tttaatatct
    29161 tgtacatttt ttatatttat ataatctttc atcatttgtc tcctttttct atcagtatac
    29221 ttttaagttc atttacaaaa gtatttatat ctttaaattg tctatacaca gaagcaaatc
    29281 ttacataggc aacctcatct aactctttca atttatccat taccatctca ccgatttttt
    29341 tggtttcaat ttcttctata aaacatttat ttatttcatt ttcaatatgt acaacaatac
    29401 tttcaatctc gtcaatagaa acaggtcttt tttcacatgc ttttagcaat ccatatttta
    29461 tcttttctct atcaaaatat tctctactat tatccttttt aatgaccatt aaaggagttg
    29521 tttctatttt ctcatacgta gtaaacctct ttttacatga ttcacactca cgtcttcttc
    29581 tgatagattt taaatctgta tgtctagaat ctattacctt tgattcttta taattacaat
    29641 aaggacattg catctatatt cctcctttgt ttaactatat ctcaaactca tctaagctta
    29701 attctgaacc tatatttact attattgtat cacaacctat tttaagtata tcattccaaa
    29761 atataacttc ttcatcccct cctcttgaaa aaaaacttct acttccatct ccaaaaatgg
    29821 taaatgagac taccttacct tcgtgaatat ccatgtctat gtctattata aaccccatcc
    29881 tttttccatt ttttacattt ataatttctt tttccattat atcagaaact ctaatcatat
    29941 tgtactcacc ctttctttaa aaataaatat gtatcgaagt aaaaaaaaat gacctatata
    30001 ggtcatttaa attaaaataa tgtttacagt ccatggatta cttatacata ttttctcata
    30061 ttctttaaag catttttttc aattcttgat acttgtgctt gagatatacc gatttcatca
    30121 gcaacttcta tctgagttct tcctttgtaa aaccttaaat ctaataccaa cttttctcta
    30181 ctatttaatt tcttaatagc ttctttaagt gatatttctt gcaaccaatt ttcatcagta
    30241 tcttttttat cttgaacctg atccataacg aatattgcat ctccattgtc ttgatatact
    30301 gggtcaaaca gagatattgg gtcttgtatg gcatcaagtg ccattacaac tgattctact
    30361 tctaattcta attcctttgc tatttctgat actgtaggtt ctttagaatt tgttctaatt
    30421 aatctttctc taacttgtag tgccttatat gctatatctt ttaatgacct acttactcta
    30481 attgggttat tgtctcttag ataccttcta atttctccta taatcatagg aacagcataa
    30541 gttgaaaacc ttacattttg gcttaaatca aagttatcta tagcttttat aagaccaata
    30601 caacctattt gaaataaatc gtctatattt tctcctctgt tgttaaactt ttttattaca
    30661 ctcaagacta atcttaaatt ccctcttaca aactgttgtc tggcttcttc atcaccattt
    30721 tttatttgta ataaaagttc cttcatttgc ttatttttaa gaactggaag ttctgatgta
    30781 ttaacaccac atatttcaac cttattaact tgcatatatt ttcagtcctc tctcaaaagt
    30841 attcatctat tgaaattatt tacatttcca atttttttat actttttaag aagaattaat
    30901 ttagcattat acaaattttt tcatttcttt ttgcaacctt gagattattt tcttttcaag
    30961 tctagatatg taggactgtg atattccaag cattccagca acttcttttt gtgttttttc
    31021 aataccttta tcaataagac caaatcttaa ttccataatt tgcttttctc tgtcacttaa
    31081 tctgtcaagt gccataacca acaaatctct atctatttct tcttcaatta ttttgtatat
    31141 ttcatcattt tctgtaccca atacatcaga taataaaagt tcgtttccat ctaaatctat
    31201 atttaaaggt tcatcaaatg atacttctgt tttcttttta ttattttttc ttaagtacat
    31261 taatatttca ttttctatac atctcgaagc ataagttgcc aattttatat ttttattcag
    31321 tttaaaagta ttaactgctt ttatcaaacc tatagttcct attgaaatca aatcttctac
    31381 atctatacct gtattttcaa attttctcga tatgtagact actaatctaa gatttctttc
    31441 tattaaaatt gactttacac tttcatctgt ttctagcttt tgtaaaagct ccatttcttc
    31501 ttctggtttc aatggaggag gaagtatatt tgcccctccc atatagtata ttccctttgg
    31561 ctttattatt tttaatttaa tacctaacat agtaataaag cttattattc tttctttcaa
    31621 tcgtaacatt ccttgtcccc cttaaatttt cacctaaaat ccaggcaata tatttggatt
    31681 cattattgct ccatattctc tatcattaaa atttgaaata ccaagtatta tatttcctat
    31741 cttttgctca tctacttcta tatagtcagc ttttaagcct attattatgc ttgtcttatt
    31801 acttccagca tgtttataag gtattattct aactctacct gaagttttat catctaaact
    31861 tcctataatt tcttgtgctt tcttcatatc tatatcttca taattaatat ttaatagttt
    31921 ttctggaata aatccttcta acacactaga cttaactatt attacatcac tttgacttat
    31981 tgggtctttt aacaaattac cactatctac taaagcttta cagcaaaaac ttttttctag
    32041 taagtttata tttatagtct ttgtaagctc cttaatgtac ttaagcattt tcaaatcttt
    32101 ataaatgtat tttaataatt cacaactcac ataagtacat gcaattaaga aagatatttt
    32161 taagtgactt attcctgtaa aataaattat aaaataagtg cttccagaaa taaaaatgtt
    32221 tactagataa aaaactaacg ctacatgaac ataatctttt ttgctcttac ttctaaatga
    32281 aattaaagtt attatactca tgattattat tttaaatggt acagtaaata gtatattcat
    32341 tgatggatac aaataagcaa cagaataggc acctcctaaa ctagccccca ataacttatt
    32401 ttttttagga ttatatcttt tagtaagaat agaagtacaa cttataataa tataatttat
    32461 tactaggttt tctataacat agtactcaat atacaccatg tatcccccct taaaaccttt
    32521 aaacatatta taagatagta tgtacaaaat tttatgtcac ttttgatggt cgaatccaaa
    32581 acaaattcta tttaaaaaga acaatgatat atgtcttata ctttatgtcg tcaaaagata
    32641 tttttttaat gccaaacaaa tagacttttt tgtagaatcc aatatatcta ggtagaagtt
    32701 attcgctcta cttttggtat atttacaaaa aaagactaca taatttaata atatatcaag
    32761 taaatctttt acttatataa aatcaaatta tatagtctgt aaattcaaat ataaattatt
    32821 gtgctagtaa tccatttgca tattttgcag attcatttag tgcttgttct ggagttatgt
    32881 ctccagctat catcatttga agttgtttcc ataaatattc atatatctct actccatgag
    32941 gtccaactgt caatggtcta tacatatgtt tatctcttgt caccatttct tcaaatcttg
    33001 ggtctccttg gtatttttca cctttagcaa ctggagctct aggtatttct ttgtggaaag
    33061 cttccattga ttctttgctt agcatatact caagaagttt aaatccaagt tctttatttt
    33121 tacatgctga cattaatgtt aaatcatcta ctgaagcaaa acttccaact gtcttatctt
    33181 ctaatgatgt tacatatccc cagtttaaat ccgggaatga tttatcaaaa gtacttgtag
    33241 cagcagagga tagcattata gtaaatgcag cttttcctgg tccaaataca ctttcaatca
    33301 tttcattact atcttttgat aaagcatctt ctggaatata ctttttcata tcatttataa
    33361 agtttgcagc tttcaagcct tctgcattat taaatttaac tgattttaaa tcatcattat
    33421 atatatctcc accagcttgc cataaatatg gataccagtt ccagtttaag cttccaaata
    33481 ctttagaacc ccaaccttgt gcaagacccc actggtcgat ttttccatct ccatcagtat
    33541 cttttgttgc tttttcacag attcttttaa aatcatccca tgtttttgga ggtttttctc
    33601 ctaatttttc tagtatatct ttattgtagt acaatacgcc tggattagca gcttctatag
    33661 gtaaaccata aataccaccc atcatttttg catcatctaa gtatagataa ttatctgaag
    33721 ttcctgattt ttctaaataa ggtgttaaat cttctaccgc acccatttct ataaattgag
    33781 gaaacatctc tgcatacatg tatcctatat ctggaccttc acctgcactt attgctgttg
    33841 catatttttc tgcgtagtta tcccatggta ctatctgaaa ttcaatttca cagttatttt
    33901 ctttttcaaa tttttcaaat atcggcttcc atgcatctgc atcatggtct gttaatggtg
    33961 cagaccatac aactaatttt tctttaccct ctttgccttt agaatcttct ttttttgaac
    34021 atcctgcaag aattgatact gatagaatcc ctaccataac taaagaaaga acctttttta
    34081 gtttaatcat gcttaatccc ctttcagact ttaatttgtc tttttcctca aatgtttaca
    34141 tttaattcag atttaatatt aacaaataag attactcttg tctaatcata aaaaactata
    34201 ctaactccta taactatatt ttttttctat acctattaga tttaattcat actaaaaact
    34261 tactttttaa attttatcaa tatttaaaaa tttttttaag tctagtcaca aactaactat
    34321 ctaaattgag caaaataaaa agaagtctat tgacttcttt ataagcttac atattatctt
    34381 cttcttctta ggaatgtagg tatctccatg ctctcatcat catcttcttc aattatactt
    34441 gcctttttag gaggctctat tttttcctca gcaattactt cttcttcaat ttcttttaca
    34501 gcttgtttta ctgttgtatt taatgaagaa cgtatgcttg atttaggttt agtatctaaa
    34561 tctaaggcac cattttgtaa tccttcaaat cctgtcgcga ttactgtaat cattatttca
    34621 tcacctaaat cttcttttat agaagctcca aatataacat ttgcttctgg gtcacaggat
    34681 tccataacta aagttgatgc ctcattaact tcaaataatc ctaagttagg accacctgta
    34741 acattaagaa gtactccttt agctccttgt attgatgttt ctagaagtgg agattgaata
    34801 gcttgtcttg cagcatcaat tgctctagtt tcaccactag cactacctat acccatatga
    34861 gcaagtccct tgtctttcat tatagtagta acatctgcaa agtccaagtt tattaaccct
    34921 tcaactgcta taaggtctga aattgattgt ataccttgtt ttaatacatc atctgcaact
    34981 gcaaaagcat ctaacattga tgtatttttt tgtactatct gtaaaagtct atcatttggt
    35041 atcgttataa gagtatctac ttttgattta agttctgcta ttccaccttc agcgttcttc
    35101 attcttatct taccttcaaa tgcaaaaggt ttggtaacta cacctactgt aagtatcccc
    35161 atttcttttg ccaagcctgc aaccactgga gcagctcctg taccagtacc accgcccatt
    35221 ccggctgtta caaagaccat atcagctcct tgaagtaact ttactatttc atctttactc
    35281 tcctcagcag ctcttttccc tacttctgga tttgctcctg ctccaagtcc tctagttaat
    35341 ttttctccta tttgaacttt atattcagct tttgatgtat ataaagcttg tttatctgta
    35401 tttactgata taaattcaac gccttttagt tgagcttcta ccattctatt gacagcattg
    35461 tttccaccgc cacctacacc tattacttta atttgtgcac attcttctaa ttctacgtca
    35521 aagtttagca tcataaaccc ccttctctca tgattcaaag ttatcgtatg tattaattat
    35581 atcattttct tcttttattt tgttaacctt taagtaacat attccacaaa aacgtaaatt
    35641 ttccttttta aatttcttac ttatattgtc aaatattact ctttaagtat aaaataattt
    35701 acgtatatat taaatgctct ttaattttta cgttttttgt tcataatatt actaataata
    35761 tatcgtctaa ttatagataa gttattaaac aatctcatac caaaaactaa tacagcaaca
    35821 tagtaaagag aaactcctaa cttttctcct atataagtca ataatatagc caatatagca
    35881 ttacctatga aaccagtaat aaaaattaca ttatcatagt tatcttcaaa attggatttt
    35941 atcgctccaa aaatagaatc cattattgca agaatagcta ctgacatata tatggaataa
    36001 tccatagaat atgtaaatgg aatatagtaa cctactacta caccaaatgc taatcctcct
    36061 aaaacccata acataaatct tcacctcttt attttacttt gaaacacaac tacattacaa
    36121 tcctttttat taattttttt tactttttct ttattttttt gtatttttcg ttttattttg
    36181 caaataaatt cactttattt ccattatttc cctgaacttt tatattaatt ccatatatat
    36241 ccttaagaga tttagcatat gattctggag agtttatagc tgcactcaat aaatctatat
    36301 ctcctatagc ttttattata aatggttgtc catatgtagt atcatttact gtaatagtag
    36361 ctcctgaaca ttttatctta cttgttgcta gtactctttc tccatttata gaaattgctc
    36421 ttgctccagc cttttttaaa tcatttaaaa ccctcaaaat atctatatca tgtataatca
    36481 aatcgtttgg attctgtccg tcttttaact ctctctcact atccctcata gtaatagtta
    36541 tgcctggccc tgtaacagtt gaataaccac ttaactcttt aacttcttca agctcttttt
    36601 gcataagtgt ttggatagat tcatttccat tcttttttgc ttctttatat tcattcaaag
    36661 tttttttgtt ctttttaaga gtttctttta aattttttat ttcttttttt gtattttcta
    36721 cctcactttc aatactttca gtttgaccta atgttatgta tactctattt gggtctagtg
    36781 attttatata agttgatatg aaaacgccta atatgaatgc tcctaatata actattttct
    36841 ttttcatact attatttccc ctctgctttg tttatatcac tatgaccatt tatttctata
    36901 ttgtcttctt tggtcacttt tactttggca ttataatatg tttctatttc ccagacaata
    36961 ccatacttta tattaagtgc tgaagccaat gtatcagaat caccaattgc ttttattata
    37021 attggttctt ttatgacagt gtcattcata taaagcttgt cttcttttaa atacatatat
    37081 gtattagaaa ctattctctg gtcgtttata gatatggcac ttgcttgtgc tgaatttaat
    37141 ttatttatca ttgacaataa taaatcataa ttatatagta tactatcatt tttatcagag
    37201 tttcctaagt cttttgtaga atttatctca atttttatgc ctgttccttc aacatctgta
    37261 tatccagcta atgtttcata tttttctatt tctgacttaa gtatgtcttg ttctccttca
    37321 ttgtttttgc ctctataagt ctctatagct ttcttcacac tattaacttc tgattgtaga
    37381 ttctcttttt ctttttttaa tgatttaagt tcttctgtaa gttgctctcc tcttttaaaa
    37441 gttgtcatcc ctctattttc tatattaata attttaagtt gtaaactaac aagaacaccc
    37501 aatacaatac ttcctatgat tatactttta tttataaatc tttttttcaa tatatcactt
    37561 ccttacaatt gtctatcctt ctggactata cactgccaac tttccatagt taagatttat
    37621 agtccctcca ttagtatttt tattttgcag gtcaatcaga attttactta ccctagatat
    37681 attataatct aagttatcat cattagaaag taagattttt atattacttc ttgtaagcat
    37741 ttctatattg ctttcttttt ttaagtttac ataatttatt tttctataaa ttccttcttc
    37801 ttttaaataa tttaaagttt taaatacatt ctccttagtt ttataatttt taaattttat
    37861 agatttatta tcatcaatgc tataatctac atctactatc aagtcttttt tactttcatt
    37921 agaacctctt agttcttcca ataaatttcc ttttttatct atataacaat aattatcttc
    37981 atcttttaat actgcaaata tttctttttc ttttaaactt ataattatct tattgggaag
    38041 ttttcttttt atttccacat tttctatata tggatttttt ataagtttat ttttcatatc
    38101 tttaaaattg tatgcaaaaa tattttcatt tagatttaca tttagttctt tcattatatt
    38161 tgatttggtc accctcttat ttccaattac atctattttc tttacatcaa ataaatcact
    38221 ttttacaatt atacatatac aaaaagataa cataagtata aaaactaaaa ctatcatgac
    38281 attattcgta tttaactttc ttcttttttt caaatataat caaccccttt ggttaataaa
    38341 tacaccttta gagcttgttt ttcctacaaa aaaattataa tatttaaagt caaatttttg
    38401 cattttatta cataacatcc aaataaggta ttaaatttta taaataaatc aatatttcat
    38461 caaaataaac atagcatact acatactatt tatatattta aatctgtata catacaaata
    38521 aacccctaat ttataataat ttagaaaatc taatatatta taaattaggg gtaaatattt
    38581 acttatcttt tctttattcc tattacctta gcttgacctt ctatacttgg agtcgtttct
    38641 ttttttattt cttttacttc cttaataact ttttcttttt ttgttttttt agaagttgac
    38701 ttttgtgttg aattgtatac tttcattatc tcatcatata taaggtctat ggcttcaggc
    38761 ttacctatag ttttacttgc atttgccata tcaaccaaaa gttctctatc tcctaaaagt
    38821 ttaaatactg ctgtatttag gctctctgga gttagatttt tttctaaaat agcaattcct
    38881 gccccttgtt tttctatact cttggcatta tactcttgat gattttcagc agtataagct
    38941 ttaggtatta ttatagatgg ctttccaaga gccgttatct cagctaatga aattgctcct
    39001 gcgctaccta taaccaaatc acttgctgca agtgcatttg ccatatcttc taagtaaggc
    39061 acaacttttt ggtatggttt aagatttata tcgctaatac ttcccataaa ttcatcataa
    39121 tagcttttgc ctgtagcaaa tataaaagct atatcttcat ttaccatatt ctttattaca
    39181 agtctcattg catcatttat ttttcttgaa cctccactac caccatagca aagtaccatt
    39241 cttttttcgt cacttattga tagattcttt cttgcaatat tttttcttga taataaaatt
    39301 tcttttctaa ctggatttcc tgtaaaaact aatttatctt ctgctgcctc tggaaatctt
    39361 ttatgcgaat cttcaaaact tgtaagtacc tttgtaactg ttttagataa aatcttatta
    39421 gttacaccag gaaaagaatt ttgctcatgt attatggctg gtattttccc catagatgca
    39481 ttaaataaaa caggtccact aacatatcca ccagttccta taactatatc tggtttaaat
    39541 ttcttaacaa tctttcttga ttgctccaaa cctttaaaaa gcttaaaaac tctttttaca
    39601 ttatcgaaat ctatttttcg cttaaaacct tgaactgtta cagtctttaa ttcaaatcca
    39661 tacttaggaa ctatttctga ctctattccc ttttccgtcc caacaaaaat tatctcagcg
    39721 tctggatgct catctcttat tttgtttgca atagctatag ctggatacac gtgccctcca
    39781 gttccaccgc ctgataataa aactttcatc aaatcatcat ctcctgttaa ttaattttga
    39841 catgctttga gatgtttaac actattccta cagctcccat aaagatggtg agtgaagttc
    39901 ctccataact tataaatggt agtgctacac ctgtagctgg cattgatgat gtcgcaactg
    39961 ctatatttaa agctgcttgt attccaattt gtgcacctat acctataaca accatacaag
    40021 caaatacatt agaagtcttc aaagctattc ttacacatct gtagactaaa actacaaata
    40081 gcataataac tattatacaa ccaatcaatc ctaactcttc tcctattata gcaaatataa
    40141 agtcattttg tggctctggt atataaaaat acttttgttt actctttcct aagcctagac
    40201 caaacaatcc acctgaacct agtgcatata gaccttgtat tacctgatac cccttaccaa
    40261 gagggtcttg aaaagggtct aaaaatgatg taacacgact aagtctatag ggttcagcaa
    40321 ttactaatgc agcaaataaa gcagccccac tccctatcat agcaaataca attttcatat
    40381 ccattccagc aacaaatatc attacaaaag taactagaat tacagtacct gctgttgaca
    40441 aatttggctg aagtattatc aacgcaaaaa atatccctgg aactacgaga agtggtacaa
    40501 caccttttgt caaagatttt attttatcat aatttttttc aattaatttt gcagttaata
    40561 ttattgtagc aaactttgcg atttctgcag gttgaaatgt taaagctcct attcccaacc
    40621 atctttttgc tccgtttgct tctatcccta aaggggtcaa aactaacaat aataatacta
    40681 ctgctatagc tccaattgct gtagcatttt tcttccaaaa actataatct attcttgatg
    40741 taataatcat aactataaat ccaagtacag catatattac attctttttt agaaaatagt
    40801 acgcatcatt gtgtttaaat gaagattgta taaaacttgc actaaacacc ataactattc
    40861 ctacaaatac taaaagcata gtagtgtaaa acactactcc atcaaattca gtcttcatcc
    40921 ttatgtcaat ttgttttttc aaattttctt taggcatcca actaatctac ctccattctt
    40981 ccatttaagt aagtttagag gtaagagatt attttaaatt gtttacatta tctttgaagt
    41041 cttttcctct tacttcaaaa ctttcataca tatcccaact tgcacaagct ggtgaaagaa
    41101 gcactatatc tccacttttt gaaatttcat aagaagtctt aactgcttct tccatatctt
    41161 ttactatatg tatgtcatta aatcctttat ttttagcaca attttctata tttgatgcag
    41221 tttctcctaa taagaccaat gatttaacat atgactttgc agtttcaaga agttcatcaa
    41281 aattacttcc cttatccatc ccccctgcta taagtattat aggtctatca taagactgta
    41341 tagcttttat agtggaatct ggattagttc cctttgaatc atttacatat attacattgt
    41401 ctaaatttct tacaaactct tgtctgtgtt ccactccttt aaatgtcata agtacatcct
    41461 ttataacttc taaatcaatt ttgcagacat atgctattgc tatagctgcc atacaatttt
    41521 ctaagttatg acctccaggt aaacttacat catctttatt tagaaatttg attttatcat
    41581 ctatatctat tattatgtca ttattttcta aatatactcc tccatttact tttttagttc
    41641 ttgaaaagta aattactttg gcattgcatt tatcagctaa agattttact atctcatcat
    41701 catagtttaa tacacagaaa tcctcaacag tttggttcat aaatatattt gcctttgctt
    41761 ctatgtattt ttccatagta tggtgtctat ttaagtggtc ctctgtaata tttaatattg
    41821 cgcttacttt aggtctaaat tcatctatac tctcaagctg aaaactgctt agttcagtta
    41881 ctaacacaga ctcttcactt gaagtttcta tagtatctat tacaggattt cctatattac
    41941 caactacata tgtatctcgt tttgctcttg aaaaaatttc tcctaccaaa ctagttgtag
    42001 ttgtctttcc atttgtacct gtaatcccta taaaaattgg attattcgat agtttaaaag
    42061 ctaattctac ctcaccaatt atatacttgc ctgaattttt taatttcaat atgaatggta
    42121 ggtctagtgg tacacctgga gacactacaa ccatatctat atcatctaca tcttctgggt
    42181 gatgtcctaa tatgtatttt atgtcattta tagatttcaa ttcatcaagt atatttctca
    42241 atttattttc atcttttata tcatttacga ttatacttgc acccaattta tctaaatgtt
    42301 taattgtaga tataccagtt tttgcaagtc ctactaataa aacttttttt ccttttaagt
    42361 ccatttttca ttccccctag ccctaaaata ctgctattat acttatccaa gctaaaacaa
    42421 ctgttataat ccaaaaaata aatacaactc ttgtttctgg ccaaccacac tgttcaaagt
    42481 ggtggtgtat tggagccatc ttaaagaatc tctttcttgt tgccttaaaa tatccaactt
    42541 gaatcaaaac tgatagagcc tccgcaaaat aaattcctcc aattataggt attataagca
    42601 ctgagttagt tagaactgaa aatgctacaa ctgcacctcc taatgccatt gaacctgtgt
    42661 cgcccataaa cactctagcc ggatatgaat taaatcctaa gaaacctaaa catgctccaa
    42721 cagttgctgc tgctaaaact gcgacttctg tatttccagc aatagaactt gcaaatagca
    42781 taaagaatac agaaactatt aatgttactc ctgatgctaa tccatctaat ccatcagtca
    42841 agtttacagc atttactatt gctacgatta taaacatcat tattggtata tataatattc
    42901 caacatttat cacaaaatct gtaaatggta tcataagttg tgaagcactt gatgatgatg
    42961 tatactgata aaaagcaaca taaaaagata atgcaacttg aaggattatt ttttgatatg
    43021 cttttagtcc aagtgatctt tttagcttta tttttataaa atcatctaaa aagccaataa
    43081 aaccaaaacc tgcaatacat ataagaccta ctgccatatc atgactaact tttactcttg
    43141 taagtccagt tattagaatt gcaacaatca ttataattcc acccatagta ggtgttccgt
    43201 ttttagctag atgtgtttgg ggtccatcat ctctaactgt ctgaccaaat ttaaattttc
    43261 taagcattgg tataaatatt ggtcctatta taattactat taaaaacgca atcaatgcgg
    43321 tgtacgtaag ctctgttatt cctaacatca taaaactcct ctcccttgtc aattaactca
    43381 ttagtttaag cttatttatt tagatattcg actatttttt ctaatctcat gcctcttgag
    43441 ccttttacta aaatagtgtc tcctgttttt actaattcat ttaacttgtt aaacacatct
    43501 tctctattct caaagtgata tatattggct gaattgaatc ctaattgttt tgcttcttct
    43561 ccaataaaca ctgaattttc acctatagtt attataatat cagtattatt catagatgat
    43621 tttccaacta atctatgacc atactctgaa atttcaccca tttctagtat atctccaagt
    43681 atcgctaccc ttctaccttt atatcttcca agtatcttta aagcagcatc catagaatct
    43741 ggactagcat tatatgaatc attaattatt gttaattttt cattttttat tatgtctagt
    43801 ctcatttttg tccctttaaa attttttaaa ccttttttta tataatctaa agaaatattt
    43861 agacacaaac caactaatat agcagacatt gcattatata tattatgttc tcctattgtt
    43921 ggtataaata tttcctcttt tttcccattt attacacata taaatgttaa actatcctct
    43981 tccatagtat aactttcaca ataaacatca ttgtttttat taaagccaaa tgtttttaac
    44041 ttatatgcat gttctttttc ttttaatgta gataaaaact tatcatctcc atttacaatt
    44101 aaagtatttg tttcatcaaa gtttgaagta atctccatct ttgctttcaa aataccctct
    44161 tgtgaaccta gtttttctac atgtgataat cctatattcg atatgactgc aattttagga
    44221 tttactatat ccactaaata ttttatttca ttgaatccag acatacccat ctctattaca
    44281 gcacactcat gttctttatt tagattaaat aaagtcaatg gaactccaaa atgattattt
    44341 aagttttttt cattttttag tatatttaac tttgcagata tggcagagta aatcatatct
    44401 ctagtagtag tttttcccac actacctgta acacctataa atggaatatc aaatttttct
    44461 ttatagtatt ttgaaatatc tcctagagcc aaagatgtat cttttacctc tattaaattt
    44521 atatccgaac tttctaattt tattccatta ctttcatttt ttataaaagt tttacatcca
    44581 ctatcatatg cagacttcat aaatgtatgc ccatctaaat tttctccaac tatggctaca
    44641 aatgcatttt gggcatttgc ctccctgcta tctataacta tatcacttac acagtcatta
    44701 taatctccat atattaactt cccattagtt gcaagtacta actcttttat tgttaagcac
    44761 tccattatta aactcctcct ttaaaacctt agctatattc tcttagcaac aaaaggcaat
    44821 gtataccgtt atatatacat tgcctacgat aactcttaac tttataatta tatactatat
    44881 ttctgttttt ttgaagatgt ttatgggtat atgttaatta tctgttactt tttagttttc
    44941 aaaatatagg gttatacttg agtcttcatt aacttttact ccaggttttg ggaatatatc
    45001 ctttaccttt gtatcttctg gaagttctat atctgcatct aagtctggtt ttaacttcgc
    45061 atcctccaat gcctttacag catctcctat ttttagattt cttacatctg gcactttaac
    45121 ttgcttcttt tcatactcag ctttttcttc ttctttatat actggtttaa ctcccaaata
    45181 ttttaaagaa tcattcatta tttctttcac tattggtcct gctgtagtac taccaaatgc
    45241 acttactcct gttggttcgt caacaatagc aagaactact atttgcgggt catcacaagg
    45301 tgctattcca acaaatgaac atatatactt tcctggagca tacttaccat ctataacttt
    45361 ttgagctgta cctgttttac caccaagtct ataacctggt atatatgcta tctttcctcc
    45421 accttcagtt acaactgatt ctgcaatttc taacattttt ttagatgttt ctttagatat
    45481 aacacttctt actttttttg gttttactgt ttcagttata tttcctttat tgtcagtata
    45541 agattttacc actcttggtt gcattaaatc tccaccatta gctattgatg atatagctgt
    45601 tataagttgt attggagtta ctgatataga ttgaccaaat gatattgtag ccaattcaac
    45661 tggtcctaca tttttttcgt tatacagtat tcccttagct tctcctggta aatctatacc
    45721 tgtcttatcc ataagaccaa atgattctat ataatcatac atttttccaa cacctaatct
    45781 gcttcctaac tcaacaaata ctgggttgca agagttttgt actgcttgtt taaattcttg
    45841 tgttccatga ggtctgtaat gtctccaaca tttaatcttt cttcctccaa cagttacact
    45901 acctgtacat gtgaattttt ctccatcttt aattactcct tcttcaagag cactggaaga
    45961 tgtaataagc ttaaatgtag aacctggttc atatgtatca cttactgctg gatttctcca
    46021 catttgataa tagcctttta ttttatcttt atcattatat ttttcaagtt cttcttgata
    46081 atatggatat attggcgttc ttgagtcatt agggtcataa tctggttttg atgctaaagc
    46141 aagtatatca cctgttttag ggttcatagc tattgcagta actttttttg cattatttaa
    46201 ctcataagct ttctgtactg ctttttcagt atagtgttga attacttcat ctatagacaa
    46261 cacaagacca ttaccttgta caggctcata atatttttcc attccttgtg gtatttctct
    46321 tcctgaagca tcagtactta ctataagttt tccaggcttt ccttttaact ttttatcata
    46381 ttgcatttct acaccagata tacctgttgc atctgaagaa gtatgtccta acacatatgg
    46441 tgcaaaattc ccatatgggt aatatctttg attatcttct gccacccata ttccacttaa
    46501 tttagcatct cttatttgac ttgccttatc atcatctatc catcttttta cttttacaag
    46561 agccatattt tttttgctta ttaaagcgta aatatcttta taatcttcat ccaaaatctc
    46621 agctaccttt tcggctgctt cttttttatc ttctacttcc acaggtttac accaaacagt
    46681 atacttagtt acacttactg ctaactcttt catatttcta tcatatatag ttcctctttt
    46741 gggttctatt ggtatatctc ttgtttgttg ttctagtgct tttgtactca accaatttcc
    46801 cttcataagt tgtagatacc cagtcctaat aacaaggcaa aaaaacaatg cacatgctag
    46861 aataagaacg agtaccagcc ttttcttact tatcctcttt acttttctca aatctattcc
    46921 ccctagttat ccacatctat atactttatt tgtccatctt ttggatagtc catattgagc
    46981 aattccttag cctttagctc aatttctttt attgatgttt tttcagtttc ttttgccttt
    47041 agttcctcta atactatctc tttttttctt aaatcttttt gtaaataata gatatcgtac
    47101 ttcatttccg agatttttga ataaccacat agattcaata ttattactga aaaagttgca
    47161 atcagtaaaa tgcatttagc tcctattttt ttcttatttc tctttttata attattcttt
    47221 tttttatttt tatattcatt tagattatgt attttataat tttttttcaa attctcaccc
    47281 cgatagtata tagtttaaat tttcctctat actatatttg ttattaatca tatttcttcc
    47341 tctaaaattt catcgctaaa ttaatacact attacacaat ctaattgtat ttttttctat
    47401 acacaacaaa aacctataaa ttcacttata cttttatatg aatctatagg ttagatat
//
LOCUS       DAEORZ010000012        46726 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEORZ010000012 DAEORZ010000000
VERSION     DAEORZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.012"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(154..933)
                     /gene="hisJ"
                     /locus_tag="KRH39_000624"
     CDS             complement(154..933)
                     /gene="hisJ"
                     /locus_tag="KRH39_000624"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBG8510548.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(1479..1829)
                     /locus_tag="KRH39_000625"
     CDS             complement(1479..1829)
                     /locus_tag="KRH39_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBG8510549.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(1845..2261)
                     /locus_tag="KRH39_000626"
     CDS             complement(1845..2261)
                     /locus_tag="KRH39_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBG8510550.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(2280..2471)
                     /locus_tag="KRH39_000627"
     CDS             complement(2280..2471)
                     /locus_tag="KRH39_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBG8510551.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(2622..3437)
                     /locus_tag="KRH39_000628"
     CDS             complement(2622..3437)
                     /locus_tag="KRH39_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBG8510552.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINMSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHSSYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RRIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLAKELYEKYKNEEYNKKR"
     gene            complement(3484..4422)
                     /locus_tag="KRH39_000629"
     CDS             complement(3484..4422)
                     /locus_tag="KRH39_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8510553.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDITISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKECFKFKNGYLDSIKLDNDIVKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(4419..5291)
                     /locus_tag="KRH39_000630"
     CDS             complement(4419..5291)
                     /locus_tag="KRH39_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8510554.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(5316..7244)
                     /locus_tag="KRH39_000631"
     CDS             complement(5316..7244)
                     /locus_tag="KRH39_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBG8510555.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(7356..7733)
                     /gene="gcvH"
                     /locus_tag="KRH39_000632"
     CDS             complement(7356..7733)
                     /gene="gcvH"
                     /locus_tag="KRH39_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBG8510556.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(7820..9946)
                     /gene="cdhC"
                     /locus_tag="KRH39_000633"
     CDS             complement(7820..9946)
                     /gene="cdhC"
                     /locus_tag="KRH39_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBG8510557.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(10003..10809)
                     /locus_tag="KRH39_000634"
     CDS             complement(10003..10809)
                     /locus_tag="KRH39_000634"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG8510558.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(10859..12226)
                     /locus_tag="KRH39_000635"
     CDS             complement(10859..12226)
                     /locus_tag="KRH39_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBG8510559.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(12257..13201)
                     /locus_tag="KRH39_000636"
     CDS             complement(12257..13201)
                     /locus_tag="KRH39_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBG8510560.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(13221..13991)
                     /locus_tag="KRH39_000637"
     CDS             complement(13221..13991)
                     /locus_tag="KRH39_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8510561.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(14047..15432)
                     /gene="lpdA"
                     /locus_tag="KRH39_000638"
     CDS             complement(14047..15432)
                     /gene="lpdA"
                     /locus_tag="KRH39_000638"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG8510562.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(15476..16357)
                     /locus_tag="KRH39_000639"
     CDS             complement(15476..16357)
                     /locus_tag="KRH39_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBG8510563.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(16393..17067)
                     /locus_tag="KRH39_000640"
     CDS             complement(16393..17067)
                     /locus_tag="KRH39_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBG8510564.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(17102..17974)
                     /locus_tag="KRH39_000641"
     CDS             complement(17102..17974)
                     /locus_tag="KRH39_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBG8510565.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(18066..18698)
                     /locus_tag="KRH39_000642"
     CDS             complement(18066..18698)
                     /locus_tag="KRH39_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBG8510566.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(18803..20479)
                     /locus_tag="KRH39_000643"
     CDS             complement(18803..20479)
                     /locus_tag="KRH39_000643"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBG8510567.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(20728..21507)
                     /locus_tag="KRH39_000644"
     CDS             complement(20728..21507)
                     /locus_tag="KRH39_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8510568.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(21817..23736)
                     /gene="cooS"
                     /locus_tag="KRH39_000645"
     CDS             complement(21817..23736)
                     /gene="cooS"
                     /locus_tag="KRH39_000645"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG8510569.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKSGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(24343..25254)
                     /locus_tag="KRH39_000646"
     CDS             complement(24343..25254)
                     /locus_tag="KRH39_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG8510570.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(25370..25948)
                     /locus_tag="KRH39_000647"
     CDS             complement(25370..25948)
                     /locus_tag="KRH39_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG8510571.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            26174..27097
                     /locus_tag="KRH39_000648"
     CDS             26174..27097
                     /locus_tag="KRH39_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8510572.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(27269..28411)
                     /locus_tag="KRH39_000649"
     CDS             complement(27269..28411)
                     /locus_tag="KRH39_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG8510573.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(28429..30183)
                     /locus_tag="KRH39_000650"
     CDS             complement(28429..30183)
                     /locus_tag="KRH39_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBG8510574.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLATYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            30319..30525
                     /locus_tag="KRH39_000651"
     CDS             30319..30525
                     /locus_tag="KRH39_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510575.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(30530..31453)
                     /locus_tag="KRH39_000652"
     CDS             complement(30530..31453)
                     /locus_tag="KRH39_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510576.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIVDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(31707..33407)
                     /locus_tag="KRH39_000653"
     CDS             complement(31707..33407)
                     /locus_tag="KRH39_000653"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBG8510577.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(33819..34232)
                     /locus_tag="KRH39_000654"
     CDS             complement(33819..34232)
                     /locus_tag="KRH39_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG8510578.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(34298..36676)
                     /locus_tag="KRH39_000655"
     CDS             complement(34298..36676)
                     /locus_tag="KRH39_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG8510579.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASNTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKSGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            36995..38446
                     /locus_tag="KRH39_000656"
     CDS             36995..38446
                     /locus_tag="KRH39_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBG8510580.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(38650..41163)
                     /locus_tag="KRH39_000657"
     CDS             complement(38650..41163)
                     /locus_tag="KRH39_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG8510581.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTSQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKALDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(41386..42207)
                     /locus_tag="KRH39_000658"
     CDS             complement(41386..42207)
                     /locus_tag="KRH39_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG8510582.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSMYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(42726..43202)
                     /locus_tag="KRH39_000659"
     CDS             complement(42726..43202)
                     /locus_tag="KRH39_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBG8510583.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(43237..43815)
                     /locus_tag="KRH39_000660"
     CDS             complement(43237..43815)
                     /locus_tag="KRH39_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG8510584.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(43919..46519)
                     /locus_tag="KRH39_000661"
     CDS             complement(43919..46519)
                     /locus_tag="KRH39_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG8510585.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT        14142 a         9041 c         5244 g        18299 t
ORIGIN      
        1 tattatttta tttagattct attgttattt tacttagaac ttattattat tttatttaag
       61 ttctattact attttcaatc atataaattt acaaaagact gtacataaat ttattttata
      121 caaacttatc tacagtcttt tgtgtgaata tatctaaata atatcgtatt tatttatttc
      181 ctctgcacag tgagaatcag aacctaaaac taatggaata tcgtattttt ttaccaactc
      241 taataagtat ccatctatat aaggctcttt gcagtactct tttctatttc cagaaacatt
      301 ataatcaagt tcataacctt tttctttaat taactttatt aactcttcaa gaattgtatt
      361 tcctttataa tcgtatggaa atacctgatt aaactttcta actaaattta aatgacctat
      421 tctttttggt ttatgtatac ctaaatctga atttacagct tttatcaatg tttcataata
      481 cttattataa acagcttcaa tacttcctaa tttgtcgatt aaattttgaa actcttctac
      541 actgtaatct acacaataat aatcatcatc aattttaatt atatgtactg ataaaagaga
      601 gtcttctaat tcctttccat atttatttaa attctccttt atcccttctt cgtatccttc
      661 tataaaatcc acttccgacc caacatttat cttgatttta ccctcatact tttctttcaa
      721 cttcttgaca tcattaaaat actttggtaa atcttcaata ctcatacaac tatcattttc
      781 aggagcaggg tctctaaatc catttgcata cggaaaatgt tctgtaaatg tcatttctgt
      841 tattccaaca tccattgctc tttgtacata tttttcaaaa tcatccttac ttccatgagg
      901 acaataaggt gaatgtatat gaccatcttt cataaataat tcactccttt tttaaagaaa
      961 aaatctaata taaatgcata aattttttaa cttcaaaata atatttttaa tcatgctaat
     1021 ttttattata tctaatatta gagtaatttt aaactgcctt ttaagtatct tatcattagt
     1081 actattttca acttaacatt aatattattt tttaacttaa cattaatact atctttaaat
     1141 agtaatactc aatatataat tacttagcat caaataactc aaattgcttg atgaggcttt
     1201 tgcagtattt aaagaatata taattatcta acattaaata atccaacctt gtaataaaat
     1261 ttactcatta aagtatatta cagaaaatac ctcaaaataa actttttagt tcatgatata
     1321 ctcttttttg catgaaatta gatatatttt tatcatttca acaatgaaag ggaggcttat
     1381 atccatcttt agacaacccc ttagataaaa tttttaataa ttaagcatca caaaatataa
     1441 aaatcgctta tatcaaacgt ataagcgatt aattatattt acatatgttt tttagcctct
     1501 aacaacattt tattagcttc ttccatatac tctatagcct tttcaatact ctttgctgtt
     1561 tcttcttttc caatagctct agccttttca acccactctc taaatccttc ttcatgagtt
     1621 tcattgtgat ttatccaatg tactaaaagt atttttaaag ttttttcatc cttagactct
     1681 gatgattcct catgactatg ttcgtggtcg tggtcgtgag catggtcatg tggatgttca
     1741 tgacatatat ctccatgagt atgttcgtgg cagtggtcat ggtcatgatg tccatgatga
     1801 tatcttacaa tctctttatc aaaaatcatt agttattccc cctgttattt tatattaaac
     1861 tcttttttta taaggtttac agaatctttt acaagtagtg ctaatggttt accttctaat
     1921 ccaactaact ccacattttc tggcataata ggtaaaagta tcttaagtgc ctcactgtct
     1981 gcaatagctt cacttatatt acaagtaact tctcccatca tagaattagg tattattaca
     2041 gaaataggtg caactattat atttgctttt ttagctgata ctactatagc attttcccct
     2101 gtagctcctt tattagcatg agctttcatc atagaactag ttgctataga attagtccct
     2161 agtgcataaa tttctacata tgtaggtaac tcttctctca aagaagaaac tatctgggct
     2221 ccaatacctc cacccattcc atctatcact gctattatca tatgaaattc ccctattaat
     2281 taattttctt gtattattat cttatggtcc atcaatctta tttctttaat tatcccatct
     2341 attatctttt gctctcctaa taaatccgtt aaataaatct ttccatcaca agggtctata
     2401 ttaaccacat tttccataac cttttctaac tcattattac ttttacaaat gaaagctgat
     2461 gattcacaca tataaatctc ctcctaatat gatttttatt aaaaatcaaa aaaagattcc
     2521 cttaggtatt tcaatacacc aaagaaacct tcatgatttc agtttttgta tattattata
     2581 ccttaagtat atcactttaa tttatgtaaa gtcaattaaa gttaacgctt tttattatac
     2641 tcttcattct tgtatttttc atatagttct ttagctaatt gcttctcata atcagtcaaa
     2701 tcgccctcca caaactcaac attaaattgt tcagctaagc cctttactat gtttttctgt
     2761 agaatatcta tgtctattct tctgccaatt tcattttcta ttccacttgc ttttttagct
     2821 gtaaatttca tagccctttc tttcagttct ggtgtcttag tttttatcaa tttaaacaat
     2881 ttttctacat caaaatccag tattattgaa ccatgttgta ataaaacacc atcttttctg
     2941 ctttgagcac ttccaattac cttcttatta ttaatagtaa cttcatatga tgaatgtgca
     3001 ttaaaacatg cagctgataa attttcccta cttattctct ctcctctatt taaattatct
     3061 gtttctatac cacttagatt caatccttta actagacctt cacttatata tctataagac
     3121 atgttgatgc tcttatcgat taaaggatta tcttctccta taattatact ataagttagc
     3181 tcattatcat gtaatacagc tcttccacct gtaattcttc tagtatagtc tatattcata
     3241 catctacact tgtccaaatc tatctcatct tccaatttct gaaaatatcc tatactcaaa
     3301 caagctggtt cccatgtata aaatctaaga gttggcttat tatgtccctt cttatatgca
     3361 gtaaatatag cttcatcaat tgccatattc attgctcctt catatgattt attatgtata
     3421 actctccact gattcatgat tattacgccc ccatttttag tataaatata tcctagaaat
     3481 cctctataga tttatttctc ttattgctaa atctatctca ctttgattta aaggtcttga
     3541 atagttatat atagtacttc ctggtctttc attatagtat ggtctattac aatccttaca
     3601 acctcttatt tcaaaaggat agcctttgtt tatgtctttt acaatatcat tgtcaagctt
     3661 tatactatct aagtatccat ttttaaactt aaaacactct tttggataac ctttatttat
     3721 catataagac attaactgta cccttctata actttctatg cttggttgat ttatcttttc
     3781 cattttagtt cctctaactg gagtaaatgc aaataaacta atcgttatat cattttcctt
     3841 taaataagta tataaattgt atatatcttc atggctttca cccataccaa ctattatatg
     3901 tgtacttatt ttattgggat atgattttga catttcagtt ataaatttta acttttcatc
     3961 atagttattt ccttttattt tttcatatag ttctttattt gctgcatcta ttgctattcc
     4021 tattttatct actcctgctg aaaaaaactt ctttatctgt tcatcatttt ctaattttgc
     4081 agatagtgaa attggcatgt ctatcttacc acttatatag tttataaaat ctaaaacaga
     4141 gttatgtgct tcttcacttg ccattgattg aatacatatt ctctttatat tttttccttt
     4201 ataagctttt aaagcatcta atatttcttc ttttgaaaac tctggccaaa ttactcttga
     4261 taatttatct tttctagtac tactttctat tgattgagaa caaaaactgc atttatttat
     4321 gcatttttct ccaatcatta tatatgctgt ggtaggaggt atatcgcttt ttttgtttaa
     4381 tatcccaagt tctattgctg tcccaactga tagtctaatc ataaaacgca acactcctta
     4441 cactcattta ttgtcatatt catttctata gccttattct tcgcagacct tgatggtaat
     4501 actattctat tgactccaca cattatagat aattggtcta gctcttttct atacactcct
     4561 cttggtctca tacaacctag attaatttca gtatctggta aattaattct agcttcacat
     4621 aaaatatctg ctaccccttc taactcagga ggttctacat tttcatactc agttcctttt
     4681 gttggaataa ggactataaa tgtaagctta ttaggaggat ttttctgtag gtattctata
     4741 atttcatatt caccctttat ttgtcctcct tttaaaccaa tacatatatg tggtgctact
     4801 tctgtatgtt cttgtattgt attatagact tcaatataat cttctttact ctttttcatc
     4861 ttataaactt ctcttattgt ctcatcatca aacactaaat caaaagaaac tatatctaaa
     4921 tacttacaca actctacaat gctatcttta tccataagac ctaaatgagc attcaatcta
     4981 tatccttgct cttttaagtt tttaatggta ttaatatgag tatttatagg gacatctccc
     5041 tcataactac aaccgccact caatagaaag ctagttatat tccttgattg tactttttcc
     5101 tcatattctt ctattggcac catattgttt aagtagtgac cgttgcaatg ggcacatttc
     5161 aaactacatt gattgcttgt agtactaaga gctagtgtct ggttaggata agcaaactct
     5221 attttatttc caaaattctt taatttaata tcaaatgcta ttttcatttt ttcagataga
     5281 ttcttattca tagtttattc cttcttaagt acattttact cttctagtaa tttctttgct
     5341 gttgggaact gctctatatt cgtatgtggt aagaaacatg cagaaataaa ttcatccata
     5401 taacttggat atacactaag ttcaacataa gttatttgat tacctatttc ttcaagttta
     5461 ttttttgcat ctttacttat aagagctaag taagaaccaa caagtgaact attccctata
     5521 taagtaaatt tttctctatc aatatcagga agtaacccta taattattga gttttctatg
     5581 tctaggttgt ttcctatacc acctgctata taaaccctat ctaatatact aaaatccata
     5641 cctaaactat ctacaagagt gtatgcgcct gaatatatag ctcctttagc tctgatgaaa
     5701 ttatctatat caacttcatt gactactata tcattttcta aatcaaattc ttctttaaat
     5761 gctaatacat actctcctat ctcatgctca ttaaatctaa ctctcttatt atctaaatcc
     5821 ctgtatattt tacctcttct atctattaca cctttagtaa tcatttgaca gattaaatca
     5881 attataccag aaccacatat accaactggt gcacattcat caataatctt taatgttggc
     5941 tctaaagtat ctttatcaat aattactttc tctattgctc ctgctgatgc tctcattcca
     6001 cagcttatcc ctccaccttc aaaagcaggt cctgctgaac atgcacagct catcatataa
     6061 tctttatttc caaatacaat ctctccatta gttcctaaat ctataaataa aacattttct
     6121 tcacttgacc atatccctgc tgataataca cctgctgtta tatctcctcc tacataactt
     6181 gcaacagatg gagctaagta cacatatgca ctgtcattta caaaaagacc aacattttca
     6241 cccataagtt ttggagattt taaaaatggt ggtatgtaag gttcttgtct taagaagtct
     6301 gtatacacac ccaagaataa acttgtcata gttgtattcc cggcagccac taatgtaact
     6361 acatcttcct tatctattcc atttctttca tatattgttt ttaatagtgg atttatagtt
     6421 tcctcaacta tagctttatg aagtgtctct aatccatttt tcttagttga atatataatt
     6481 ctatttataa catctgctcc atattttatc tgtgcatttc cagaagatgc tttatctact
     6541 acctcttttg agtataggtc tactaaacaa acaactactg aagttgtacc aatatctatt
     6601 gcaactccgt ataaggaatt ttctttattt ccttgttcta tatttatgat tgtaagtttc
     6661 ttttgttttt gaacatatgt tattgtaacc ttaaaatcac tctttctaaa tacagttggc
     6721 atttttctaa gtatatccaa tctaaaatct atttcattat atcctaggtt atttctaaca
     6781 tatctttcca atctatctac atcacttata ttatcatcta aatttggttc ttccatttct
     6841 atgtatttct tttttatatt tgttttaaat tgaaggttat gttcttcaat tattttttta
     6901 gctctttcaa aaatttccct atcctctttt ttattactac cttctatctt cataccatgc
     6961 atagaagatg atactttaga aggaacttca atttctatat ctgatattac ctttgtacaa
     7021 caagctagta tataaccttg ctcccactca tcatctgtta tatgtcttgt tttttctgta
     7081 tcaacttttc cattcaataa ttttacttta cacttcccac aagacacgtt tccattacaa
     7141 ggagcatcta tgaatatatc tgcatttctt gcaacctcta gtaatatatc cccctcatta
     7201 caataaacct ctttattgtt tggtgtaaaa cttactttta tcatgttatc cctcccaatt
     7261 tgttctatat atgataaaag gaaagtatag ctaaatttgc gtgcactcaa attttactat
     7321 actttcccca attataataa tatcttaatc tttaattatt ctaatccagc ctcgtattct
     7381 ttatcactta ataacgtatc aagttcagcc acatcagcta gttttacttt taatatccag
     7441 ttttcatatg ggtcttcatt tatacattct ggctcatctt ctaatttttc attaacttcc
     7501 aatacctcac cattaacagg agatattaaa tcagaagcta ctttagaaga ctcaactact
     7561 ccaaaatcta ctcccttagt tacagtatct tctacttctg gtgtttcaac aaacaatatt
     7621 tctcctaatt gatcttgagc atagtctgtt atacctatat atacaacatc tccatcaatt
     7681 acttttaccc actcatgatc tttagagtat tttaattctg gtaataattt catttcgata
     7741 gacccccaat attatattat tgattaaatt acttaaatca tattaatatt atatcatatt
     7801 attttaaatt ttccatcaat tacattacac tttccatagc taatgctgga tgaccttttt
     7861 cttctaagaa agctaatact ccttcagaat cgtcagctat agtttcatca caaaccatat
     7921 cacagaagtt ttctattcca gtcatttctt taactgtagc atttaattta tcagctacat
     7981 aatcttttaa ttcttttggc atccaaacta ttctttctgg tccaccttct gcataagcga
     8041 attttttaga agatatgaaa tgtcttccgt gtcccataaa tcctggagtt tgaactcctc
     8101 cacctgtcat agaagcaagt tctccgaaag tcataccaac tggagtaaca cttgcaaatt
     8161 ctctgttaac tacaacaaat ccatttgctt ctggcattat accacagata cattcgaagc
     8221 atccacaaga agtcattggg tcttctagta tagagtataa tgtaacagat tcaactgcac
     8281 cttgagatat ttggtttaca gtttcattta ctgaattcca tacacctgtt ctatcatcta
     8341 agcactcgcc tttttctatt ggttgacaag gacctgttgg gtctaattct ttagtagcct
     8401 tagcatctaa ccagctaact gctccacaaa gaccaagtct ttcaggagta acaacacaaa
     8461 cgtgagctgg tgcaaatgat tgacataagt tacaagaata gaaagtgtca acactttcat
     8521 caactaatga agctaatctt tcatctctag cattgtactt agctattgct tcacctttta
     8581 attcagaaac tttttcagca tctgtaatta tagttatttc acacttatca actactgatt
     8641 caaattcatc taacatctta gcatataaaa cttctcctat atgctcaagt ctaaatcctt
     8701 tatcaaatgc atctttagag attcttaccc aagccatatc tctttgtcca acgtgcatta
     8761 caccttctat atagtttaag aagtagtgga aacgtctttc aagaactggc tcgaaatctt
     8821 cttgcatatt tttaccagct attttaacta ttacagcaag tggtaatctt aatacaccat
     8881 ctgcatcaac ttcatctata tttggtccta ttatttctat cttatggtct tcaacttctg
     8941 ttgcttcttt cttaacaaca agctcccaag cttcagttct gtttccacca aactcagcaa
     9001 acatatcgct ctttctaatt ctctcacctt caaatgctgc tgcaaatgaa actgggatag
     9061 gtatttcagt aacttttatt tttattcctc tagcttctaa tgaagtagct actatcttat
     9121 cgtaatcttt ttgagttaat aggttcattg gaacttcaag aactgtttgg tcagttataa
     9181 ctgggaatcc taaagctatt gctccagcac ctgcagatac aactaattca cttaatggtc
     9241 caaatgcatt aacaaatgca ggaactctat tagctgtgta ctctaataat ccgtttaaat
     9301 caccaggagt taatccaccg aatattaatg ctgctcttac tgcaacagat acaacgtgta
     9361 taacagaagt tacatcatat cctagaggta taactcttag ctcaagaccc atttttactc
     9421 cagcttctat agcttggtct ataactttac caactaagaa agttagtaaa cctttagatt
     9481 ggtaatcttt tataactttt gctgctgatt ctgcatctgg acactcacca agaacaactg
     9541 caacaccagg tatatctcct gtaactaatg gtacaccaag tgatctgatt atagggtcag
     9601 ttatatgacc tgcacatggc tcactatatg gagcatccat agttgaatat tttaatgctt
     9661 caattacttc tgctgctaaa gctgtagcta aaccagcatt aaaagcatgt tctagtaaat
     9721 gagttctatt tattaaagat tttactactt ctaaagcacc ttctaagtcc cctaaagtgt
     9781 tcatcttttg tcctgttgca gcatatatac aaggtaatga atatgctgta tcagggaaag
     9841 caactttatg ctcttttcca ttcttttcta tagcttcagc taacatagct tgagccgcac
     9901 ctaaagcttg ttctgaccct gtaaagatta tattatatag attcattcta tttccccctt
     9961 taatatttac acctattttt tattaactat accctaaaaa ccctagaaag caaatccacc
    10021 ttcatttaac tctaagaaag agtctgcata taaagatgcc ccgttgatta tgtcacaaga
    10081 ttttacagtt ttcattaatt ctgggtcaca tggattcatt atagctgaac taaatccatt
    10141 agccattgcc attgctaaga atgcactatc taaaacaggt ctaacatgct taggacatcc
    10201 attagatacg ttagataatc ctcctgtagt taaaagtccc atctcagtca tcatttttat
    10261 agcttctaaa acttctactt gtttttcttg catacctttt ataactaagc ataatgggtc
    10321 aaatagtata tcagttggct ccattcctaa cattaagcct ttttctaata tttcttggca
    10381 gtaagccata cgctcatcat tatctcttgg gataccttct tttgcacata aacctataac
    10441 cattgcttcg tattcagctg ctatatctat taaatcaaat cttgagccag catcagcaga
    10501 gtttattatt ggttttgcat tagttctatc ataaacttta agtccagctt cgatagcttt
    10561 tttattagct gtatctaatg ctataggaac attgttgaac tcagattgaa gtaatttgat
    10621 tccccatgtc attatttctt cgccatctct ttcggcaggt cctatattaa aatctatata
    10681 gtgtgctcct gcttctaatt gctcttttgc tctttttaag attggagctg ggtctctttc
    10741 tgctaatgct tttcttattg aaggtgatat acaatgtatt ctctcaccta taatcataaa
    10801 tttttccatt tataatgtcc ccctttaaat ttaatattaa tagttagtta atattttatt
    10861 agtttgtttg gcatgctttt tcttggtatc cttttaagaa cttaggtaat tccatagatt
    10921 cctctggtcc tataactaca ttccatcctg gtaagttatc ttctatatca cctttaagta
    10981 ctgcaacttt tcctggaatg ataaggtctt tacaattagt tacttcttct accttacttt
    11041 ctttaatgaa agcagagatt gagtttccac caaatttacc agcagcccaa gatgtaagaa
    11101 ctgaatatcc acctgcatct ggtattacta accatactgg aacttttgat ctttctatat
    11161 caccagcaac tatgaagtaa gttaatgcaa agtcaacagt aactaatact ggagaatttt
    11221 catctgggtt gttgattgga taaatctttg gttcaactct cattggtctt tgtgggtcag
    11281 tgtatatgtt ttgtcttagt gcaaataatg gtaatgcctt agcataacta atatcatcta
    11341 ttactattat agaaccgtat tttatagtga atattgatga taaagcaact tccatcatag
    11401 ggtttgaatt agataatcta tttgcaaata ctattgaagg atatccaaat gttctatctt
    11461 gctcttttaa agctgttctt ctaacttgta tagcattagt gtaagtatct tttatgtttt
    11521 ctcctgttac atctaatact aattctttat atccagcagc ttgaattaat tcaactgttt
    11581 catataattc ttctaagcta ccagctttta cacctaatgg taaacttgct cctttaacta
    11641 cttctatcat atctttgtag ttctctttag ttgctccata taccattggt ctagcatctt
    11701 ttaatacttc aacagcttct ttaactactt gtgcatcatc acaagctaaa atataagcta
    11761 cttctaatcc actgcctttt attttatcta ttaattttaa atacgcatct ttatctccac
    11821 aatactctaa aacagccatt tcaactttca tttgttcacc aattcttaca tagtcaactt
    11881 ttttcatgtt agctatttta gcatctactg cttcatcact catacaagta cagaatgaaa
    11941 ctgcatatct atttctactt actaatgttt tttcatgtct aaataatact gtttctccac
    12001 ctagttcata ctcagatgca ccagcaccaa cttttaaagc tttcattaaa ggtgcagtag
    12061 cctctgataa ttttgctata gcgtcatcag acatatgtgg gcatttacct acttctacag
    12121 ctcctgaagc aaccttcata gagaaagcca tacaagttgg gaatccacaa tccttacagt
    12181 ttttctttgg tgttaattta aatatatcta aagcttttaa tgccattatt ttttccccct
    12241 aaccttaaca atttaattaa gctaatgcgt taaccaattc ttttatagtt tctattgatt
    12301 ttggatgacg aagtataact gcgtttgaac cacctactaa aacacttgct gcagttgaaa
    12361 cttccattgc tatagttctt tcttctggac aaccccaatc tggctcatct tctattggag
    12421 ctatagcttc tttaacatga cctacttcaa aagctactgg tgttatgata ggcatttgta
    12481 atgtcttatc attttgacca aatgctgcaa gtctaattct atccatagta gatgcaacgt
    12541 actcatatcc ataaccaact gctgaacatc caacattcat aactatattt tcacctttaa
    12601 cgcctagttg agttaacaat acgtttaatt gtttagctag gtttatatca acagaagatt
    12661 cagctcctac tttatgtgca taagccatac cagctgatgc tcctactcct ttgtagttgt
    12721 cttctactgc tgacataaat aagcagttat gtccatccaa tgtttgagct aatttttcaa
    12781 ataatttacc atctttttca tgatttcctg aaccagctac aactaaaggt aattttattg
    12841 cttcaattac tgccttagct acatcagcac attcatcaac tgacttatct aatccatttg
    12901 ggtcagaacc atcaaatttt aaacaaataa aatctgcttg tgtattagct tcaacatatt
    12961 tagcccactc tactggacaa ttagctacat ctttgtataa ttccttatat gaatcagtcc
    13021 agctttcagg ataaacgtct gatatttgta tccctatttt tggagaatta cctacttctc
    13081 catcaaaact ataaaaaggt aatacatttt ccccacctaa tttaattgct ttttccccta
    13141 ttcctacttc aacttctgat atttttccag aatatttttg agtagacatt ttaaatgcca
    13201 tatttttttc ccccttaagc ctattttaat tctaatttag caaaaatttc tttcatagcc
    13261 actttagatt ttgaatcttc aggtaaatta actaaaggaa tacctgtaga atcatattca
    13321 tatatcaatt catccatagg tacaacaccg attagctcta ggttatgctt ttcaatttct
    13381 tctttaacac catcattcat aaccccattt ggaactttat ttacaatcaa aagtattctt
    13441 ccaacactta actttagttc ctcagctaaa tctcttattc tagcaacagc ttgtatgcta
    13501 cgtcttgagc aatcactaac caataaaagt gtatcaacat gtctagtgac ttttctactt
    13561 agatgttcca taccagcttc gttgtccatg actaagtatt tgtaatgacc agatattttg
    13621 tttacttgtt ctctaagtat cccatttaca aaacagtaac atccttcacc ttctgaccta
    13681 cccattacta ataagtcata tccttcgcct tcttcgatta tagaatttaa tctaaattga
    13741 agatattgtg cctttgtcat accaccaggg aatgcattgc ctaacttttc tctttgattt
    13801 acttcttctc ttatttctcc tattgttgct tcaacttcga tacctaatac ttcatttatg
    13861 ttagcattgg catcagcatc tacaactaat actggtccct ttttgtcctt aaccaaataa
    13921 tcaatcaaaa gccctgtgag acttgtttta ccagttccac cttttcctgc aacagctata
    13981 ttgtatccca ttttaagact tcccccttaa atctagccac agagccttat gtactctgtc
    14041 tgtctattat aatttaggta cagaatgaac acattctcca tgtacatcat gaagtgcttc
    14101 cattagccct tcagataaac ttggatgtgg atgtattgca tcccctactt gttctactgt
    14161 taatcctaga tgaacagcaa gtgacaactc tgtcaataaa tctgtagcat gaggtcctac
    14221 tactgctgca cctattatct tatcagtttc tttgtcagct ataactttta caaatccttg
    14281 gaaatgtcct atagcttgtg cttttccaag ccctctaaaa tcaaattgac ctacattgta
    14341 ttctacacct tcagcttcaa gttgcttctc agtcttacca acaccagcaa cttcaggttc
    14401 agtataaaca catcttggaa ttgctctata atctactacc ttagtcttac ctaaagcatt
    14461 ttcaactgct acgatacctt ctttagaagc aacatgtgct aggaaaggag tatctattat
    14521 atcgcctatt gcgtatatac cttctacatt agtttcaaga tgttcattaa ctactacttt
    14581 tcctctttcc atttcaattc ctatatcttc tactccagaa ttgtcaaggt taggtcttct
    14641 accaacacat actaatgcat attgtgcttc tataaccttt ccattagaaa gagttgcaac
    14701 agccttacca tcaactactt cacaagtttg tactccaata cctgtaataa ccttaatttt
    14761 atctttctta aattgtctaa gtagttgttt agctacatct ttgtcttcat ttaatagtat
    14821 ttggtcaacc atctcaacta tagttacttc tgttcctaag gctctaaaga actgccctat
    14881 ctcacatcct ataactccac cacctactat taacatagac tcaggtatct cttcaagtcc
    14941 aagaacttca tcactagtta ttacaacttt tccatcatat gggaacattc ttggtacaac
    15001 tggaacagaa ccatttgcta gtattatctt atctgctttt atagtttcta ctgttccatc
    15061 atctttagtt acttctatag tgtttttatc aattaatttt ccaaaaccat taactaagtt
    15121 tactcctctt ttttcaaata agaactctat accacttatt aattggttta caactttatt
    15181 ttttctttcc attatggcag tgaagtttgg cttaactgta ccatctattt ctataccaaa
    15241 atcctttgct tcttttacag tattcaatac tcctgaagaa gcaagaagtg ctttagttgg
    15301 tatacatcct gcatttaagc aagttcctcc aactcttctt ttttcaacaa ctgttacatc
    15361 tgcaccaagc atagaagctt ttatagctgc tacatatcct cctggtccac cacctactac
    15421 tactatcttc atctttaatt cccccaaacc taatataatc tacaatcttg caatactata
    15481 aacctgcggc atccagtata gcaggtatat tctcttctgc cccaattgcc attatatgaa
    15541 caccatcaca taaatcttct tcttttaatt gtttgataag ttcccctgcc atttttattc
    15601 cttcacttac ccagttttct tttccagctg ctcttaatct ttcgatttgt tcatctggaa
    15661 caaatattcc tggtacattt gcagtcataa atttagccat tcctggtgat tttaaaggta
    15721 ctattcctgc taataccttg caatccatat cccttgttaa ttctctaaac tttctcattg
    15781 tatttatgtc ataaagtgct tgtgtttgga aaaatttagc ccctgctttt attttctttt
    15841 tcatttttaa taattgaact tctattggag aatattctgg agttactgat gctcccagat
    15901 aaaaatctgg agaacctttt aacttattac cagccatgtc cgtacctgcc attaaagtct
    15961 cagcagtttg aagtatcccc acagaatcta aatcaaacac tcccttagct tgtggatggt
    16021 ctcctacgct agtatggtct cctgttagtg ccaacagatt gtttatccca aatacacctg
    16081 ctgacaacat ttctccttgt atagctattc tatttctatc tctccctgtt atttgaataa
    16141 ctggctctaa tccagcatct tttaataatt tacatgtagc aagagaagtt gctctcataa
    16201 ctgccgattg aaagtctgtt acatttgctg catgaactct tccaactaac ggctttgcac
    16261 attctattaa atgtgaaagg tcagtccctt ttggaggagc catttcagta gtaactgcaa
    16321 attttccact ttctaatgtt tctcttaata agctcatctc taatccccct tttaaccttc
    16381 taaaaaaaac ttttatgaat ttgctgctgc ttctttcttt ttctttgcac ttaaacttct
    16441 tggattattt tgcttagaat agtcttttgg tggtcttatt tctgctaagt tatctaattg
    16501 tcctatagct tctagtcttt catatatctt tatccaagca caatcattct ctgagtttac
    16561 ttcacactta ccatttttag ctccaccaca agccccattc ataagacctt tagcacacat
    16621 agttactgga catatcccac ctgtccaacc aagttcacag tctccacaag ccttacaggc
    16681 ttcttcatat tctccaactc tttgtacttc ccctataaac aaagtattgt tggctggata
    16741 aactggctta tcttttaaat tcttaactat tgtctgtgta ccatctccac atgctaaaga
    16801 taaaactgca tcagcttcct tagtctcttc ttttagagcc tttaaatcct ttttagactt
    16861 taaaaggtta caagcagggt caagcattac atatcctaag atttttttgc cttcaccttc
    16921 aagagtttcc ttcattttta aaacttcttc ttccccacca cttttacaag ttgctgcaca
    16981 ttgattacaa ccaactaaaa ccagcttgtc aaaatctttt aagtacccta gcacttcttc
    17041 taatggttta ttttcagaaa ttatcatatc tacttccccc ctaaaccttc cacatatata
    17101 tctatttatt gttttgtaat ttacaagcct taactacatg ttttgcaagt atagatgttg
    17161 taactgaacc aacccccgct ggaactggag ttatcattga tactttgtct aaaactgcat
    17221 ttgtatcaac atctccacat aaatttcctt cttcatcaac atttatacca acgtctataa
    17281 ctaccgctcc atctttaaca aaactttcat caaccatctt agctctacca atagctgcta
    17341 ttagcacgtc tgcctctgct gctacacctg atagattttt agtttttgaa tgacatattg
    17401 taacagttgc atgttcactt aataaaagca ttgatactgg ctttccaact accattgatc
    17461 ttccaagaac tgttacctta cttcctttta aatcaacatt atagtgtttt aatatctcaa
    17521 ctactgctgt aggtgtacat ggtggaaatc cacttttatc cccttccatt accttagcag
    17581 agtttattgg actaaaacaa tctacgtctt tttccggtgc aataacatat tttataactt
    17641 cttcatttaa gtgttttgga agaggtctaa aacataatat accattaaca tttttgtcat
    17701 cattaactct ctttaataca tcaatatatt cctcttgtgt tatatcctca gctaactcta
    17761 acacttctgt tgttatacct atgttttgac atctctttag tgctcctctt tcataagata
    17821 aatcatcact tcttgcccca actctaacta ttgttagttt aggatttata ccttctttaa
    17881 ctagtaaatc gacttctttt attaattctt cactaatctt atcagctaca ggttttcctt
    17941 ttataatttg tcctttagtt gacattcctt ccatacattc acaactcccc tacaagcaaa
    18001 cttaaattaa tttattctat ataccacata ttaataacat agtatctact ttcgtcattt
    18061 aaaaattatt ttccaagagc tttttcaact ttggcataaa cttcatcaca aatttttaca
    18121 ccatcttcaa ctaacttatc tacttctgtt tttaccttat taacatattc tttatcttgt
    18181 atagaattta tatttataac tacatttaat tgtccaccaa gtatagcagc tcttaaacac
    18241 tgaactccta caccaacatc acttatagca agtcttgaac ctttatcaac taaatcttca
    18301 tgtaacttaa tagcttcata gcaaactctt actatgttta atggtacttc acaagcaact
    18361 tttaatgcct tttccattgt ttctgctttt attctttttt cttcttctgt ttctttcgga
    18421 agcccgtaag cttttgataa tggtaagaaa cattctgcat cgtcatctat catttttaat
    18481 aaatcttttt ctaactttcc tgccttactt aatatctctt ttacactttc ttcatactca
    18541 gcatacttct tcttccctat tgtaaggtta cataccatac tacctaaagc catacctatt
    18601 gcacctacta gtgcagcagc tcccccacca cctggtactg cttctttgga agatagtact
    18661 tctacaaaat caacacaagt tttatctgct attttcatga taatcctcct tgaaaggatg
    18721 taggaaaata tctcaaatta ttcgagatat tttacgaact attttaagat atctcctact
    18781 ctttatttat aaattaattt ccttagaata atccagctat tactccattt tcatcaacat
    18841 ctattttttc tgctgctgga acttttggta atcctggcat cttcattatt tctccagtaa
    18901 gtgcaactat aaatccagct cccgctgaaa ttgttgcttg tcttacagtt attctaaatc
    18961 ctgttggtct acctaattta gtttggtcat cagttaaaga atattgagtc ttagccatac
    19021 atactggaac tttagtaaat ccaagttttt ctaagttagc tatttcctta tcagcttctg
    19081 gagtataatc tacaccatct gctccataaa tcttagtagc tatagcattt atcttgtctt
    19141 ttatagataa atcatcttca tagcagaatc taaagttatt ttcttcttca tccaataatc
    19201 ttaaaacttc tttagctact gcaattccac cttctccacc ttttgcccat acttcagaaa
    19261 gagcaacatt aacacctaat tccttacact tgtcttcaac aagtttaagt tcagcttcag
    19321 tatcaagtgg gaatctgttg attgcaacaa ctgctggtaa tccatatact tgagttatgt
    19381 tttcaacatg ttttaataag tttggaagtc ctttttcaag agcttctagg ttttcgttat
    19441 ttaagtctgc tttagcaact ccaccattgt attttaatgc tcttacagta gcaactatta
    19501 taacagcatc tggttttaag tttgccattc tacattttat atctaagaat ttttctgctc
    19561 caaggtcagc accgaaacca gcctcagtta ttacataatc tgcaaagtgc atagccattc
    19621 ttgtagctat aacagagtta catccatgag ctatatttgc gaatggtcca ccgtgtacaa
    19681 atgatggtgt tccttcaaga gtttgaacta ggtttggttt taaagcatct tttaataatg
    19741 cagccatagc accattagct tttaattgtc tagctgttac aggttcacct tcatagctgt
    19801 atccaactat tatgtttcct aatctttctt ttaaatcaga tatatcactt gccaaacaga
    19861 atgctgccat tatttcagaa gcaacagtta tatcgaatcc atcttgtcta actgctccat
    19921 cacctttttt acccatacca tcaacgatat ttctaagttg tctatcgttc atgtctacac
    19981 atcttctcca agttattttc ttagggtcta ttctaagtgc atttccttgg tgaatatggt
    20041 tatcaagcat agcagctaaa agattgttag cagctcctat agcgtgaaaa tctcctgtga
    20101 agtgtaagtt tatatcttcc ataggaacaa cttgtgcata tcctccacca gctgctccac
    20161 ctttaacacc aaatactggt cctagagatg gttctcttaa agcaactaat acatttttgt
    20221 ctagtttagc aaatgcatca gcaacaccta tagtagtagt agtttttcct tcacctgctg
    20281 gagttgggtt tatagctgta gttagtatta acttagcttt tttaccagtc tctcttttta
    20341 gtagattgta gtcaactttt gctttgtatt taccataaag ttctacatca tcttcaccta
    20401 gtcctaattt ttttgcaacc tctctaatat cttgaggctt agcttcttga gctatttcaa
    20461 tgtcagattt aaatcccata actgaatctc ctcccacaac ctaaatagat ttaaaatata
    20521 atttttgtaa aaattgtatt aatatgtaaa aattatattt tataagataa ttatacatgt
    20581 aaaattactt aaaaactagc tactaagcta ttaaaatatt caaacttaat agctagttat
    20641 ttatctaatt acttacttat tacctatcta ttgcctatgt ctaaccttta accaacttaa
    20701 aatcccaaat caaaatttct atctatttta tttatcctta agtgacatta atttatcttt
    20761 tattgcttta atctgttctt tcgttgtttc actagaatca tatggagatt gattggctct
    20821 atcagaatca ataacatctt ggttataata aataaatcct aaagattctc catcttctaa
    20881 gttttggatt ataaattctt catcttcctt atttctaatt ttatttccta ctacaaatac
    20941 tttatttaca cctatatcat gtccaagttt cttaactttt ctataagtct gtaaacttct
    21001 ctctcctggt tctacaacta ctataaaagc atctacacct tgggctgttc ctcttcctaa
    21061 gtgttcaatt cccgcttcca tatccataac cactacatct ttattttgta gtattaaatg
    21121 tgagcagagt ctcttcaaaa gaacatgctc tggacataca catccagtac caccagaatc
    21181 tacggttccc aatgtaagta atcttactcc attgtattct ttacagaaat tttcagggat
    21241 atcgtctact ttaggattca tcttaaacat ttttccaaat gaacctacag aagcagctgt
    21301 tctatctgaa actaattttt tcatttctga tataggtact attgattcat atacttcttt
    21361 tggaaaacct agtgctaaag ctaaattagc atcagggtcg gcatcaacag caacaactct
    21421 gtatccgtct tcagcaaaca tcctagagag cattgaagaa aaagtcgttt tacctactcc
    21481 acctttacct gttattgcta tcttcataat ccctaccacc ttttattact atttgctttt
    21541 tagtagttaa ttttacagtt aaatattttt tatactctat atttttatac actaaaaata
    21601 aactacagct agtccttaca ggaaccaatt aaatttaccc tactagcttt tcaaagcagc
    21661 atgtatagtt ttattattct ttaatattgt ttatatttta tttattcagt atattcatta
    21721 tattttgatg ttctttactt taatatttga ctttattcaa ctttattcaa ccagaaactt
    21781 cttataaaaa cgtttattct ataacttcac tattttttaa gcttcagcct tttctgccat
    21841 tttttcttca actaatgctt caaagtgaac acgttttttc tctatatctt ctattattgt
    21901 agctgctaac tcttttggat ttgtatttac agtaaatgta gctccaactt tatcttttaa
    21961 ttcactagtt aatatatcca ctgctcttga agaaccagct attggaggca ttattcctaa
    22021 ataagtgtct ataccagaag ctacaacata gcatcctata gcaactgctt tttctgacat
    22081 ccactctgga gctataccta caactggaag gtcacacata tccatatcta agtaattagc
    22141 tgctgctcca actaaatcta gtatacgact tatatcaaca caagaaccca tatgtagtac
    22201 tggtgggatt cctactaatt tacagacagt tgcaagacct ttacctgcat atttttcagc
    22261 cgcatcagat tccattaaac caaacttagc tgctgctgaa gctccacaac ctgtagtaac
    22321 tactattatg tcgtttttga ttaattcttt cattattgtt acgtgagcct catttgaaac
    22381 tcctttagca ttgttacatc caactacacc agctgctcct cttaatactc cagattttag
    22441 acagtctgtt aatggcttaa ctgttcctgc tgggtctata tgagagttta caactctgtc
    22501 taattgattt actactgctt ctacactata cccaactgtt gcgcctgatt ttaattcagg
    22561 tataagtact tttgatttat ctctattttt aaagtttaaa atagcttctt taactattgc
    22621 tttagcatca tcaagtgctt gttcttctct aaattcaata taagttgaac ctgttatctt
    22681 agcttttgga gaagttgtta caaatttagt atggtaacaa tctgcaactc tagctaatgc
    22741 agggaatata cattgaacgt caactattac agcttcaact gctccagtta ttattgcaag
    22801 ttcttgttga tggaagtctc ctgctatttt tacaccatgt ctcattgtaa tctcattacc
    22861 tgtacaacac atacctgcta agtttattcc atcagcacca acttctttag ctaaagctac
    22921 taagtctggc tcttctgatg ctaaaactat catttctgat aatgctggct catgtccatg
    22981 taatattatg ttaactttat tttcttctaa tactgctaaa ttcgcctctg ttcttcttgg
    23041 cactggagtt ccaaataaga tatcgcttag acgagtacct atcatagaac caccccaacc
    23101 atcagccata gaagttctaa gactcatgtg tattaaacta tctatatcac cagtacatcc
    23161 tatatgagta gagtgcatta ttgttgcaat ttctctatct atagctcttg gctctatagc
    23221 gtattctttc catatttttt gtcttggttc tggtgcattt tttataaatc ttaatgtccc
    23281 aaaaggtttt ccaaattcca ttaatgcaat ttctgccact tcatgtgcta catcatagat
    23341 atctctacct tctgtctcta catcccattc tttagctaaa gtaattaact tagcttcgtc
    23401 tcttatttta tagtttccat ttggatcagc taatgctaat gtatgtgcta tatctctagc
    23461 atggtctgaa tgggcagctg tcccaccagc aaccattctt gcaaagtttc tacctactat
    23521 agtatgctca tctgctccac ataaccccct tggagtctta gggcttattc tacatggtcc
    23581 cattccacat attctacagc aaacaccttg aagaccaaaa ccacattgtg ccttaaagcc
    23641 ttcttttcta tcatacattg tttctacgcc ttcttctcta gctttagcta tcatcttctg
    23701 gctgtttaaa tcaattgtta acattttttc atccatattc ctatcccctt tttttgattt
    23761 ttaaaatccc ccaggtgact gacttcatgt cataaaataa taaaataaat gctttctaag
    23821 ttgattatat actaattttc agtgctttgc tttagattta aatatttttt taacaaaatt
    23881 agataataca taaaaatttt attttctaca cttaatttaa gttgatttta tcgcttgttt
    23941 ttggctattt gttaatatct aaacctactt ttttgatact tcagtttaat taaattatgt
    24001 tttgattttt ttaaatcaaa tttgaaattt ttctagtcat ttttatatta ttgcagattg
    24061 tttgtgtatt tattatcttt cactactgtt tggacttatt acagattttt actccctaaa
    24121 cttaattagt aaaaatttct tagaatagca ttttttggac taattttttt gaattttttt
    24181 atttttccta ctttaatatt aattatagct tcaaattgaa catttgtata tattcatttg
    24241 aagctataat tattttttat ataaaacttt tttaatacag cattaattta gacattttga
    24301 ctattttttc tactttaatt catttatttc aagcgattta tcctatttca tatttttagc
    24361 tacaatagcc ccatatgcaa tagaatataa tttagattca tgtgaatcag ctcttgacac
    24421 taaaactata ggagcttttg cacccataat tatacctgct gatttagagt ctgccatata
    24481 tgtaaaggct ttccctattc cattaccaac ctctattgtt ggaaccaata tgatatctac
    24541 atctccagat acttcacttt taaacccttt tatctcacta gcttcttttg aaacagctaa
    24601 atcaaaagcc agaggacctt ctacaattac attttcacca aattctcctt ctttacaagc
    24661 ttttgctaac atatctgcat ctacagttgc ttgcatctta ggatttactt tctctttagc
    24721 tgccaaacat gcaactttta cagtttccat tcctaaagct tttgcagctt ttatagaatt
    24781 ttttaatatc tttgcctttt gttcataatc aggagctata ttcatacctc catctgtagt
    24841 tataagtaat ttatggtatt tttctaactc atataccata acatgactta ataaactatc
    24901 agttctaaga ccatagtctt tatttaacac ttcttttaat atgacagatg tatctaataa
    24961 tcctttcatt acaaagtcgg cttctttact tgatactaat ttaactgcta tttctgcaca
    25021 ctctttgata tcatccttat ccactatttt gataccactt aaatcaaatc ctatttcttt
    25081 tgatatttct cttattttgc cttcatttcc aaccaatata ggagtaatta tatttctttc
    25141 tacagcatcc tttattgcca ttaaaacttc tttatcatga gctgctgcta cagagagtac
    25201 aattttctct cctcccttaa gttgttctaa aatatcatct aattttttta tcaaaagtaa
    25261 cccctccctt atatatattt aactaaataa taccatattt gctatacctt ttctcaattt
    25321 attttttatg ttataaagaa tttatataac taaaagacag tttaatattt tattctggga
    25381 tttcatcaat aactcttaaa agctttgcag gaacaccacc tacaatagta ttttctgcaa
    25441 cattttttgt tacaactgct ccagcagcga taattgcatt atctccaata gtaactcctg
    25501 gaactacagt tacattagca ccaatccata cattttttcc tattgtgatt ggagaaggat
    25561 aagttgtacc acgtttctct ggtgcaatac catggttaag tgtagccaat accacattat
    25621 gcccaatttg cgtattatct ccaatctcaa tccccccctg gtcttgaaaa tggcaactgc
    25681 tatttataaa aacatttttt cctattgtga tgttttttcc acaatctgta taaaatggtg
    25741 gaaacataaa aaatgattca tctacctttt ttccaattaa ctcagaaaat aaactacgga
    25801 cttcttctgg ttcatgataa gaagaattta acttacttgt gattttcatt gcttcattac
    25861 tcaacttaac cataaattca tgtatttctg atttactaga aacaggtttt ctttgattga
    25921 tatgtgttaa aaattcattg atattcattt tatacatcct ttcaatatgt gctttaattt
    25981 atttgtacat tctaaataat attttaataa aaattaattt aatttatttt tattaaaata
    26041 tcattgcttc ttttagtgta atatacaatt atatctatat caaatatcta tattaaatac
    26101 ttaactatac ttaaaaagca tataaattga tataattata taatagataa gttaatattg
    26161 aggagggata attatggaaa taagagtatt aagatatttt ttagcaattg caagagaaga
    26221 aagtatatct ggtgcggcaa cctttttaca tataacacaa ccaactcttt ctagacaaat
    26281 tatggaactt gaagaagagt tgggagttaa attattggtt cgaggaaata gaaaaatcac
    26341 cttaacaaat gaaggtaggc ttttacataa aagagcagaa gaaattatat cattagttga
    26401 taaggttgaa ttagaatttg acaatcccaa tgagattatt agtggtgata tatatatagg
    26461 aagtggtgaa actgaagcta tgcgacttat agctagaact gcccatcatt ttcatcaaaa
    26521 ataccccaat gtaagatatc acctttttag tgggaatgct gatgatgtga ctgaaaaatt
    26581 agacaaggga ctgcttgatt ttggtatttt aattgaacct gcaaatatac agaagtatga
    26641 ttatgcacaa attcctgcta ctgatgtatg ggggttacta atgcgtaaag atagtgccct
    26701 tgcatcacaa agcacaataa atccaaaaga tttatgcaat atacctctta ttatttcaag
    26761 acagacactt gttgataaag caatttctaa atggcttcaa gacagtaatg aaaaactaaa
    26821 cattattgca acatacaatt tagtttataa cgcttctctt ttggtagaag aaggtctggg
    26881 ctacgctctg tgtttagata gactcgtcaa tactacagaa aacagtaatc tttgttttag
    26941 acctctatct cctcctttag aagctaaatt aaatattata tggaaaaaac atcaaatatt
    27001 ttcaaaggca gcagaaaaat ttttacttca aatacaaaaa gatttttctt ctatcatgtt
    27061 ggatacatct aagttggata cacctaaaga aaaataaatc ataataaaaa gtgagagtgt
    27121 ctcaaaatga actttgcact gtataaggca ctctttttta tacaaagtaa aatatatgtt
    27181 catcatttca gcactaaaaa agaggcttat ctctattttg agatagcccc acttaaccaa
    27241 taaaattaaa tacaacttaa caatatcctt acttatttat tccaagttct cttagacaag
    27301 caatagtaat ttcatcttgt ggatttaatt cataagctct ttctatatag taagttgcat
    27361 catcaatatc accattattt aaataagcca ttcctaaatt gcaaagtatt tctgggtctt
    27421 cctttatctt agccgcttgt tcaaaatact ctattgcttt atctaaatta ttcttataag
    27481 cctcacaaag ccccaattcc actatagtat ccacctgatt aggctttatt atcaatatct
    27541 tctcaagata catcttagcc tgctctattt cgcccatacc cttatatcca agtgctatca
    27601 taaacagcaa gttccaccaa tccatatgtt cttcttctaa tggaagtaac ttctctaatc
    27661 cttccttaaa cttaccttgg aatacaagtg tatatccttc ttcatattga actttaaagt
    27721 ccatcttgcc cagattctct tgtacctcag ctactaaatc agcatctaac cctagcctta
    27781 acgcttcttc ccaagtcagc ttagtcttta aatattgacc ttgattataa taatggtagc
    27841 ctaagtgata ataacctaat gcaaaattct catcaacatc aattaccttt tcaagcttag
    27901 ccatggcttc aagtaagaat gcattcattg ccttatcatc catatctttt tgatactgtt
    27961 tagctatctc ttggcaaaca atcgcatagt tatacattgc atctaaatca tctggataca
    28021 ttgttataaa tgcttttata tatattaaag aatctgtatg ttcaccaatc tcaaagtatt
    28081 ttctcgacat atatcccata tacgatttca aatccaaatc aatatctttt gataaagcat
    28141 ttaaaaactt ctcatactct gcattgtttt taaacttact atctattcca attatatata
    28201 tcattgcatc tgctatagac attgaattta acttatcttg agcagtcttt tcttttatcc
    28261 cttttactaa tacctcattt tttataggta catctaatcc accttttggc acttcatagc
    28321 tttttaattt aaactctcca ccatgtttta tagttataaa agctaattct tctgcttttt
    28381 ttagcaaata tttttctatc ttaaaatcca ttttatcctc ctaaatactt aaatctctac
    28441 tttatgagtt ttagatttct caaatatttt tttatcatca tactcaaata agactacatt
    28501 ttctacttct cttatttctg tatttatatc attttttaaa tccacaacat taaatctaaa
    28561 tggctcaaaa tgaacatatt ttattatatg tttagctggt ttatcaacaa aactaggtaa
    28621 atatgttgct aagttatcat tatcttgtaa aaatacatgg catctattct taaaatcatc
    28681 cacatctagc ttattaaaaa actgtggtat atttgatgtt ttacccagtg atatatagtc
    28741 atattttaat atatcattga atacatcatt atctatgttg attttttcat tatagaaatc
    28801 catcaatatt ttatataact gattctttcc ttgtgccaaa tcaaagtagc cattttcatc
    28861 aaaatacttg gcaaaaacct caaagaattt aaaagggctt tgtttataaa atctgcctat
    28921 aatatatctc atagataaca caaagttttt agaattgtag tatctctcca gtatatcttc
    28981 tatatccttt agcttcaaag tttcttcata gcttatataa ttattgtata aaaactcata
    29041 tggagcataa tccttatatt taaaaccatg ctcatctgca acttttctca tacctgtccc
    29101 tttaatcatc tttaaaaagc ctaattgcag atgctctata cctagattaa atacatcatt
    29161 aaaagaattt tcaaagctct tataatcttc ataaggcagt cctgcaatta agtctaagtg
    29221 ctgatgtata tttctataag atgctacagt ttgtacaaca tgagacaact ttttaaaatc
    29281 atcccttctg cctactgcat caagtgtttt ttcattcgtt gactgaaccc ctatttcaaa
    29341 ttgaaaaaga ccttctttac aatcagccaa aaactcaagc atcttatcat ctatcaaata
    29401 cgctgtaact tcaaaatgat aagttgtata tccattatca tttttcataa gaaaatccat
    29461 gatttccata gccacttttc tatttgcatt gaatgtccta tctataaact ttatttgtga
    29521 tactcttgca tctattaaag cttttaaatc actctttatc ctgtctaaac taaaatacct
    29581 tagacctgga atagtagatg ataaacagta ttggcagtta aaagggcaac ctcttgatga
    29641 ttcataatac actattctat tttcatattc ttttggattc aagttttcat aaggactagg
    29701 aatctcatcc aaatcttgaa gtaattccat aggcttattc actattatct cattgccttg
    29761 tctatataca agtccattta catcatttat ttccatcttt ccttgcaagt gaagtactaa
    29821 atctctaaaa attaattctc cctctccata aagtatataa tccacaaaat catacttttt
    29881 cattgcctca taactatcat aactcacttc cggtccgccc aaagctattt tagtattagg
    29941 tctaattttt tttagattat cacaaagttt aactatatct cccacattcc agatatatgt
    30001 tgagaataat attatatcat attcattttt atatatatcc tttaaaatat agtccaaatc
    30061 gttgtttatc gtgtattctt tcatattaac ttctatcaaa tctcttacaa attcttttag
    30121 atatcttata gccaaatttg tatgaataaa cttggaattt aatgttgtta ataaaatttt
    30181 caaatatttc tcacctcttt ttaagattat actgtctttt tgtacccctt ataggttaaa
    30241 atagtatatg taaagtctaa ataatatcta tataattaat ttattaattt caagtaatgt
    30301 gaggaaagga agttttttat ggaattaatt gaaaaagtat ctatcaactc tttatcaaaa
    30361 agagatttac tacttattat aaagtctctt gaatacacta atgaaaatac tcaaattagt
    30421 gatttcattg accttaggaa taatatagta aaggaattat gttttttaac tgacactaag
    30481 gaaaaagatt ttttagatta tctagaaaaa aatagcacac tctaataacc taaacctttt
    30541 taaactttaa tatataaata cctgatttag aatttatttt tatgcttaat atattatcat
    30601 taaaggtttt cgacataact gttatttgat taaatatagg gtctactata gtattacctt
    30661 cttcaagtct tataagactt cttgggtcta tgcttttatt atatgcctta actccaattt
    30721 tatattttct caatttaaca acatattcgg acatagaaaa aataaaatct atattatctt
    30781 catttaattt actatcttca cccaaaatgg ataatataga tttttttatg ctattaattc
    30841 cttcattttc tacagttcta tcttccatat atacatcata tagaacattt aaaacattta
    30901 ataaaatact ttcttcatca taatcttcag atttcaaatc aatgtactta tcaattaatt
    30961 gtattgcttt aatccttgcc atctgaaatt ttacaacatc tgatttgtct aattctatag
    31021 aaaatcctat tatcttgtct ttaatactct gtagtaattc gacatactct attttactat
    31081 tatttataag attgtgcata atggaattat acactacaga acctaataaa taatcaaata
    31141 aaagttcttc ttttttaaaa taaataacat acttttttat tagtttaata acctcatctt
    31201 cacttttttg aatattagta tttgataaaa ttagtggtaa taaaatattt aaaaaattga
    31261 tatttcctat tttttctcca taaaatctat agtaaataag ttcatctctc agtctatttt
    31321 cgtctatata cacacatctt tgcgttttat catctattaa ggattttgta tttattatag
    31381 ttaaatcttt aagagcccta cttgcactac taaaagaaga tgtgtagact ttttttagta
    31441 attcgtcatt cataaatata tcctttctga aaaattgaat gtaaataaat tgttattaaa
    31501 aaaaaagaga cagcccgttt aagaagctgt catatatagg gggatagtag tgtacaaaaa
    31561 tcattgtaat ctactatcta tatattatta tacgatacta cttttttata tgtaaagcat
    31621 cagttttcga catccctata ttttacagtt atacaaattg cgtactaagt tcaattttat
    31681 tctttttatt tcaacatttt atcatgctac attctttcag gagcgtgcat tcccaacaat
    31741 ttcagagaat tttctaaagt ttgttttgtt gctaaaacta atgcaagtct tgcttttctt
    31801 atttccaaat tttctactaa aattggattg tcatgataaa atttattaaa cgcttgagct
    31861 aaatctagca taaatctagt cactatatgt ggctcatttt tctttagagc aagtaaaata
    31921 ttcttattga atgattctat aactctaaga acttctgcac tatcttcatc agctaataga
    31981 ctataatcta tatcagaagt cacttctact tctgcttttc ttaaaactgc acagcatcta
    32041 gcatgtgtat actgaacata tggtccagtc tcaccatcaa aactaagcgt tctttcccaa
    32101 gagaaggtat agtctttaat tctactatta gataactctt ggaatactac tgccccaaca
    32161 cctacttgct tagatatttc atccacattt ttagcatttg ggtttttagc tagtataatt
    32221 tccttagttt tatctatagc ttgatttaaa gcatcttcta aaaatactac tcttcctttt
    32281 ctagtcgaca tagtaccttc ttcaagtgca accataccaa atccaacatg aattaaatct
    32341 tttgcccaat catatcccat aagctcaatt accttaaacc attgttggaa atgtaagttt
    32401 tgttgagaac caactacata tatacacttg tcaaaatcat aagtttcttt tctatatatt
    32461 gcagcagcta aatctcttgt catatataac gttgaaccat cattcttagt aataagtgct
    32521 ggtggcattt tatactcttc taaatcaaca attcttgctc ctttagactc ttttaaaaga
    32581 tttttctctt ctaatagttc aataactctg ttcattttat cagaataaaa actctctcct
    32641 gctaaagaat caaattctat atctaaaagt ttatacactc tgttaaactc ttttaaactc
    32701 tcatttctaa accattgcca taattcttgt gcttcttcgt caccattttc taatttatta
    32761 aaccatgctc ttgcttcatc ttccatttct gggtgttgtt cagcttcatc atggaatctt
    32821 acgtataatt ttagtaattc tggaattgga ttagactcta ctacttcttt ttctccccac
    32881 tttttaaagg ccactattaa cttaccaaac tgagttccat agtcacctaa atgatttatt
    32941 cttatagtct tgtatccttg cgaatcatat atcttatata aagcattacc tataactgta
    33001 gttcttatgt gccctatatg gaaaggtttt gctatatttg gagaagaata ttcaactatt
    33061 acagttttat cttttccaaa atcactatgt ccgtaatttt ctttttcatc taaaactttt
    33121 ttaattacag tttctgctaa ttgagattta tttacaaaga aatttacata tccgcctaat
    33181 tgtattactt ttgttatctc tccacttggt tctatacttt ccgccaattc acttgctatc
    33241 atgtttggag cttttctaaa cactttggct aatttaaagc atggaaaagc atagtccccc
    33301 atatccttat ttggtggaac ttctataagt gcctctatct cttctttcga taaatcttct
    33361 attttttctt taaggcaatt tgatattgcc accttaaaat cttgcattct gttgctcctc
    33421 tctacattgt tcctggttta tattgtgctt atctctttat gttaaatcaa atttcgtgtt
    33481 ttttcaagta tttcattaca gtgtaaaaca ctttagtaat tatgttttct gtatatatta
    33541 aaaatactat aattgcagtt tttaatcaca ctattattga ttttttataa gacttttttc
    33601 tattttattg tttcttgtac tatccagttg actttatata ctttttttaa tcttttatct
    33661 attttcatac gtcaatgcta attttatata cttttctttt aatcttttat taattttcat
    33721 acatcaatgt taactttata tacgtttttt taatctttta tctattttca tacatcaatg
    33781 tcagctttat atactttttt tactctttta tctattttct ataaatcttt ttcactctta
    33841 acttttattc cttcttcttc taatagcctt gctgttatac ctttccctgg tatcttttta
    33901 cctgaaaaac taccatcata tattagatta catccacaag aggggctacc ctcttttagt
    33961 attgcttcgc tacatacata taaatttgct atttttagag tttcattagc ccctcttaca
    34021 aagttttctg ttacatcctt attatcttta gtaattactt ttccatcaac tatctcagca
    34081 ggtggtcttg gtgtagatag accacctaac tgctctgggc atacaagtgt aaattctttg
    34141 ccttttaaat attccttgac tttttcatta tcattattat cgccactata tttacaattt
    34201 attcctatta aacatgcaga taccaatatc ataaaaatgt gctcctatac aaattatata
    34261 tattctcata caaattacta atgtattctt atacaaatta ttttaattta acaattgtta
    34321 ctcctgctcc accttctcca tattgaccac ctctttgtga ttttacatgt ctatttcttc
    34381 ttaaaatgtc ttgtagccct gcttttaata ctcctgtacc tattccatgt attactgtta
    34441 cagactctaa acctgccaca tatgcatcat caagatactt ttctacttcc attatagctt
    34501 cttctaggtt taaccctcta aggtcaactt cattttttac actaccagat tttgacctta
    34561 taatgtttct cgttgattta gttacatttg ttgaaacatc tttgcgtgtt ttttgtaaag
    34621 atttaaaagg tagagtcatc ttcattatac caatttgcac tactgcttct tttctctttt
    34681 tatctactga aactacacta ccattttgat ttaaagttat taccttaact tcttcacctg
    34741 gctttaagtc ttttatctcc ttatttgata cctttggaac tatcatactt tttactgttg
    34801 gctgaagact tcccatagag ctagtcaatt cttttctaag ttcttctatc ttacgattct
    34861 tttcttttga agctctttct tgttcaagac ttctaagttc ttttattata atatctactt
    34921 cttctttagc ttgtcttata attgaaaatg cttctgattt agctttttca atcatcttat
    34981 ctctttgact gaccaatttt tcaagttttt cgtcatactc tactttcagt ttttcaatct
    35041 cttcttttaa tctttcagct tcttctctat cttctactgc tttgattcta ttcttttcaa
    35101 cattctgtaa cacatcttct aaagctatgt tttctgtatt tatatattct ttagcacgtg
    35161 aaatcacata atcacttagc ccaagttttc tagaaatctc aaacgcattt gattttccgg
    35221 gaacacctat aagtaactta tatgttgggc ttaaagtttc tatatcaaat tcaactgctg
    35281 cattttctac acctgattta gtaagagcat aatttttaag ctcactatag tgagtggtag
    35341 ctatacattt agctcctaca gaatttatat cttctaatac tgctattgca agggcagctc
    35401 cttcaacagg gtctgttccc gctcctaact cgtcaaatat aactaaagag tctgctgtta
    35461 cgttttgtaa tatagatact atattcgtca tatgagatga aaatgttgat agactctgtt
    35521 caatactctg ctcatctcct atatctgcaa atacattatc atatacacac atactacttc
    35581 cataatcagc aggtatatga agtccacttt gtgtcataag tgcaaataga cctacagttt
    35641 ttattgtaac agtcttacca cctgtgtttg gacctgttat caccaatgta tggaagtctc
    35701 ttcctaaata aattgtattt gctactacct tctttttatc caacagtgga tgtcttccat
    35761 tttttatgtt taaatattta tcctcattta aagttggttc tattcctctc atctgtatag
    35821 aaagttttcc ctttgaaaat gcaaagtcca atcttccaag tatctcttga ttagagatta
    35881 aatcttcgct cacttctcca accatagcag aaagctctga taaaattctt tctatttcct
    35941 cttgctctcc tagtttcaac tgcctcaatt cattgttcat ctctactata gtcattggct
    36001 ctataaaaag tgtcgcacca gaagatgact ggtcatgcac tataccagct acttgcgacc
    36061 tatattcaga ttttacaggt actacaaatc tatcacctct tagagaaatt attgcatctt
    36121 gtaagtactt ttgataagta gttgaactta ttattgaatt taattttgac ctaatggatt
    36181 gatttttttg ggctattctt cttctgatat ctctaagtgt atttgaagca ttgtcagata
    36241 tttctacttc actaattata gcattgtata tctggtcttc tatgtccttg tatacataca
    36301 gagaatttga tagagattgt attattggat aattaaaatc ttcttcatca ctactagata
    36361 agctatttct taatcttcta gccactctta aggtatctgc tatcataatc agacttcctg
    36421 gatcaagaga agcacctata taagctctct ttaccttatc ttctatatca tgaatacctt
    36481 ccaaccctac tgaacctctc tttataataa ttgattgagc ttcactagtt tcttctagca
    36541 tagatttcac ttctacaaaa tcagtatttg gtactaaatt ttctatatat tttagaccta
    36601 aagaagatga ggctttcttc tttaacaaat ctattatttt attgtattct agaactctta
    36661 acgatttctc attcattttc tatattcacc atcctcgata ttttaacttt tttatttaat
    36721 tttaaaaatg tatgttttca agcatttcca ataaaaactg caagttttat ttctttttca
    36781 tttctaaaat tattatattt tttctctttt agatatattt tacacatatt ttgtattata
    36841 ctatgtatgt agctatctat atattataca taaacatgct ttatttgtaa tataatctgt
    36901 taaaaaatct tctttaatat ttttaaagga gtttatttta gcttataaaa aaaaatagct
    36961 acttatatag ataaattaag gaggaacatg agtcatggga aacgtattag aaggattaaa
    37021 accagaaagt gtttttaaaa actttgagaa aatatctcaa attcctagag gttctggaaa
    37081 tgaaaaaggc ataagtgatt ttttacttag ctttggtaaa aatttaggtc ttgaaactat
    37141 tcaggatgaa agcttaaaca taataataag aaagcctgct acaaaaggat atgaaaattg
    37201 tccaggtgta gttcttcaag gacatatgga tatggtttgc gaaaaagaaa agaatgttga
    37261 acatgatttt cttaaagacc ctataaaatt aagaattgat ggtgacatga tatatgctac
    37321 tggaactact ctaggagctg acaatggtat agctgttgct atgggtatgg ctatattaga
    37381 agataatact ttagagcatc ctgcactaga agttttagtt actgttaatg aagaagatgg
    37441 tatgaatggt gcagatgcac tagaccctag cttaattaaa ggtcaatata tcctaaatat
    37501 ggactccgag gaggaaggat acttattagt tagctgtgct ggtggtaaaa cttgtgttgt
    37561 tagtttacct gttgaataca aggaagttaa aggtgacaaa caaggattat tagttgaggt
    37621 tactggacta ttaggtggac actctggtat ggaaattgtt ttacaaagag caaatgctaa
    37681 caaagcaata gctagagtat taagtgtgtt gaatgttgat tatgaattag cttctgttga
    37741 tggtggtact aagcacaatg ctatacctcg tgaagctaaa tgtgtaatag ctgttaataa
    37801 agctgatgtt gaatctgcta aaaaacaaat aaatgacatt ttaactgcat ttaaacatga
    37861 gtttactact tctgaccctg gcatgactta ctcagtagct gaaacttctg tagacaaagt
    37921 tcttactaaa gattgtaaag aaaaagttgt tcaaatgtct tgcttaactc ctcatggagt
    37981 tcaatcagta agtcttgata tagaaggatt agttgaaagt tctactaact tcgctataat
    38041 tgaaactaaa gaatctacta ttgaattttt aacttctgtt agaagttctg ttatgtctat
    38101 tagagatgaa attgctgata gaataagact tttggctcaa gctttaggtg ctaattacga
    38161 ccttatagct caatacccag cttgggaatt taaaaaaggc tcaaaattag aaaaaatatg
    38221 cagtgaaact tatgagaaat taactggtaa agttcctact gttatggctc tacatgctgg
    38281 tcttgagtgt ggattattac ttgataaact tcctcatgct gaagctatct ctataggacc
    38341 agatatgttt gatgttcata ctccaaatga acatgttagt atcccttctg tagctaatgt
    38401 ttgggattat gttattgaaa tattaaaatc tatgaaccaa tattaaattt gagttttgat
    38461 tctaagtaga ttttaatttt aagtaatcta tataaaatta actctgtaaa ttaatactac
    38521 tgttagtata atatttactt tcaatattaa cttatagaaa taaagcctaa tgatagataa
    38581 taacatgtat cattaggctt tattgtaatt taaatattat taagctttat tctgttttaa
    38641 aattaattct tatactattt taaatctatt tcttccttgt ctcttagaat cataaagagc
    38701 attatctgct atttgataca gttcatcata ctcttgacca ggctttgcta taactacccc
    38761 catactaata gataaacttt ctccatttgg caacttaatg agatgaatat gattgtatac
    38821 atcattacat ttattaataa gttcttcctt atttattata tcattgatat aaacactaaa
    38881 ttcatctcca cccaaacgac ctactatatc attttttgca aaactttcat caagtgcctt
    38941 tgcaacatca ataagtactt tatctcctaa caagtgacca taagtatcat taatcgattt
    39001 aaaataatca atatctataa tcaataaagc accatttttt tcagttgata aattcaacaa
    39061 attttgtgaa gttaattttc taatatatgc tgcattataa atattggtta aactatccct
    39121 ttgagcctta tctaacaatc tattcttttc ctctttttca gaatcaatat tacatatttt
    39181 cccgattaca tacattggta tttttctatc attataaatt atttttgaag taactctaat
    39241 ccaatgaaaa gtatcatcaa gcataataga atacatttca gttgttccat tttcttgtga
    39301 ttttattaca tcaaaaaact ttttattata ttctttcctt attttatctc tttcattttc
    39361 tatattgcca ttttcacttt ccttatcatt tttatccaaa ccataataag tttcaatacc
    39421 cttttctggc atagaaatta tcaatttatc ttgcttataa tcatactcaa aaaagtattc
    39481 acttgtcatt tcatacaact gagtatgttt tttgagttct aaagatatct tctcgttcat
    39541 acttgtacga gtatgtaaac caaatgccat aaatccaata atacaaatta ataaactggc
    39601 tacaataaaa atagtctcaa ttggattctg ttgcataata taaagcaacg agaaatcttg
    39661 tctatatgta gtattttggt agacaattga ttgcattttt tctgatgaaa tacttataat
    39721 agctttatta aatatattca ataattcttg tttatctttt ttaattatac caaaacaaat
    39781 actctttgct ttataagttt gaggaactag acgcacattt ttatacttag gttgattcat
    39841 atagtactgc atagaataac cttcgccata agtgtaatct gcatagcctt tattaacagc
    39901 ttctatacac tcttctaaat tatcatacca aactatattc tcttcaaact gaccataata
    39961 attaatacct ttggctagag ctacacgttt tccatctaaa ctattctcat ttaatttatt
    40021 attaatcatc atagtatatt gagtagaaag aaaaggccga gacatagcta tattatgttc
    40081 acgagagtat tcataatcat aaatcattcc tgctacaata tcaatttcac gctttttagt
    40141 catgttttct aaatcttctt gagattttac aggcacaagt tcaaattcta agcctgtttt
    40201 atcacatata tgatttaaca aatcaattga tatacctctt aattcacctg tatctttatc
    40261 tatattttga aatggtggtt tgtttaaaag tactcctact tttaattttt ctgtctcttt
    40321 aatgtaatta gtttcttcat cagataaaaa catttgatta ttctctggtg taaagtattt
    40381 ttcatataaa gtagttgaaa aatatgggtc tttctgttca atattattaa ttgctgagtt
    40441 aagcttttga gttaaatttg tactaccttt aggagtaatg aaataaaatg gttttgatga
    40501 aaattttgct acagtacgta caccttctat tgggttcata ctcacattaa gaagtacatc
    40561 tgctttacca ctctttagtg cttctagttg ctcatcattt gttttacaat ataccaattc
    40621 aggtgacata agattcattt tacaatactc atctaattct ttaagtctag tatcagaatt
    40681 ttctattaca gcaatacgga tttttttcac atcttgtgaa ctcattatgt tatcaatatc
    40741 tgtattttca tataatgctt gtagtactgt atttgctact ccataattat tccctgcata
    40801 attatatatt ttttgcattg gttcattgta taacatacct cccattatat caatttcacc
    40861 attttctaac atcttcatta attttgtaat agattcattt atctctccag gaacttgaac
    40921 aaattcataa ttccatccag tatactgtgc aatttctagt aaatattcat atgtataccc
    40981 aaaatagtta ccttgctcat ctatttcagt taaacctttt tgaataggat aagcaacacg
    41041 aatggtacga ttatcattac ctatagcgtt gataccaaaa gaaaatatgc aagaagtgaa
    41101 tatagatgta attacaataa ttaataaaag taattttttt aaattcacta cacaatcctc
    41161 catatagtta agatttacag tataaattac tgcataaact tgtagacatg taaaaataga
    41221 ttagtatata aaaggatata taaatctact tagaaattta aatatcctta atatatacaa
    41281 tatatttaaa ttataactca tttatatgtc tattacaaca aatttaccct gctaaatacc
    41341 tactctatat cccataattt tacaagttgt agtgaagttt aagttctatc tcacctcaaa
    41401 tttagcagtg tttctattta cagttctttt atatttcaca tctggataac ccaatataaa
    41461 tgaagtgata acagcttcgt tctcgctcat tcctaacagt tcttttatct ttggctctac
    41521 atttgatgcc ataacaaaaa atccattata acaaattcct aatccttgag aataggccat
    41581 aagttccata ttagatgcag ccaatcctcc atctacataa gcacttccac ctagagacat
    41641 atcacctaca actaccataa gtagtggggc atcataaaat aaagaatcat ttccgttctc
    41701 tttgtatctt ttgtacattt tcttaaatgt atttttatac atagaacgta cagggtcatt
    41761 gtcatcagta tctaatgcca aattataaag accttgtatt gccagttctt tcacttcttt
    41821 tagcttttct tttactaata tgtatcttat aggctgtcta ttaccacctg ttggtgtgta
    41881 tctaccagct tccactatgt tttttatttt ttcttcttct acctctatat ctttatattg
    41941 tcttatactt cttctaaatt taataaaatt caataacttt tcgggttcta attcaaaact
    42001 ttcacgattg tattctatta cttcattttt atcaaatcct tgcatttcta ttgcatctgt
    42061 tgggcaaatt gccatacagt gaccacagtt aatacagaga atatttttaa cctttgcttt
    42121 atcatcctta acttttattg catttacaag acagtctgat tcacacatac cacagcctac
    42181 acatttttct aaatttatgt ctatcattcc aaattattcc tccttttaaa aaatttactt
    42241 tagtataaaa caaatagttg tctaagtcaa tttttatttt tttaacaata tactagcctt
    42301 aactacactt taatggagaa aaatatacat aaattggaac ttaaattaga acttattcaa
    42361 agtaaaagat aaaagaagta gatgtctaaa agtgaacttt ttagttaatt agatattctt
    42421 tattgtatga aatcaagtgg atatttatca tttcaacaat aaaaaaggct tacctctatt
    42481 ttggatatgt ccctgtcaag tagacagact aaaaaaaggc tatctttatg cactgagata
    42541 gccccttttc ccaactacta ttgtttattt taatattata tcaataaatt taatttgcta
    42601 taacctgtaa gttacatttt aatttgatgt aactttaaat tacattttta agtagtatct
    42661 ttaaattata ttcttaaaat atatcattaa actacatttt aattaatcta aaaactatat
    42721 cttaattaat ctaagaaact tacattataa tcttttagtt tcccattatc atatacaatc
    42781 tttgcactaa agatatttcc agtcttagcc caatttaaga acagcctgtc accttcccac
    42841 atatttaaat cgaatatttt atcattatca acccaaatta aatttccttc tttacaatca
    42901 tttaaatctt tttcagataa ttcaccttca aactcatctg agaaatacaa aaacatatac
    42961 caatcttctt catctttaaa ttttggaaaa gttatcaacc ctttatatga caatgattta
    43021 agagtaagac cagtctcttc ttttacttct ctaattacac attcttctgg agtctctcct
    43081 tgctcaaact ttcctccaac tcctacatac ttaccttcat gtatatcatc tttcttttta
    43141 tttctgtaca gcatcaaagt cttgtcatct ttttctatat aacaaatagt tgtaagtttc
    43201 atgtatctcg ccttgctttc tcttttatat tctcaactaa ttattttcag cactttcaag
    43261 cacatcactt atattcttca ttgcattaag agaaagctct gcaaattcat caaatggaat
    43321 ccctatatct tctatacttc tcattgcatc tctatttaca tttgaagcaa atctcgtttc
    43381 tttcattctt ttaataacag acttagtctt tacactggta agttttttat ctggataaac
    43441 taaagctata gcagttataa acccagttat agggtctgct gcatatattg ctttttccat
    43501 taggctagtt cttgtaactc ccattccatc atgatgacca agtattgctc tatacatttc
    43561 ttcgtcaccg aagttttctt ttttaagcag ttctactgtt gttgtagcat gtccttcaca
    43621 tatttctcct ggctttcttc caactatatc tgaatccaaa tcatgtaaaa gacctgccac
    43681 tgcccactct tcttcttttt ctggctctag tcttttggct aattctctca taacagcttc
    43741 aacagcataa caatgttttc tcattctatc agtttctaaa tacttaaata aaatttctaa
    43801 agcttcttct ctcattttca ccaatccccc tgttttttca catatatgat atttttatat
    43861 attaatacac ataaattcaa gtttttatat atattcatgt atatttttca tgttatatct
    43921 attctacacc tttatagtag tgtcctgcaa ctgttccaat ttcatctaat ttcttataca
    43981 agtctgtagc cttttttcca agtttataat tattactcaa tacctcaatg tgagcttcaa
    44041 taaccttttt aaccatctca ctatcttcaa ctgtaaaatc tatcctaaat acatctattc
    44101 caatttcctc taactcatat agattgtcca acatacaagt aaccttcgaa ttgtaaattg
    44161 tacttctaca gaatatatct tgtgttatcc tataacttgc atccgtaaag tctcttaaag
    44221 cataactact ttccttacat ttagcaactc tcttgtcctt tttacaatct ctaacaacta
    44281 ctcccatagg acagtattct gtaaccatca taggagcata tccatatatt actgcttcaa
    44341 tttctacatc tgtatatttt aaagtttgtt taatttcatt taagtttagt tcttgtgaca
    44401 aacaaactgt acttgctccc tcttctttga agtgatttat actttcacta ttgaatgcat
    44461 ttaagtagca atctatattt aagtcttttt tcttaaagta ttccatagag ccccagttac
    44521 caatttgaac cttattaatt ccttttatat tatccaatat ctcaaattgc ttgtactctt
    44581 tatttctaac aatccttggt gctgaataga ttaatttttt gtcagtgact gctgtattat
    44641 agtggttctt attcaaagta tctattttac ttgtatttga caaagtatct atgttatttg
    44701 tatttgaacc cattagttct ttaggagtac ctaaagacga agaagtatct aaatccatag
    44761 aaatacttga atctatagaa atatttgaat ctatagaaat acttgagacc atttgtaaag
    44821 cttttgataa tgtatttata tcttcataat agattaaatc tacattataa tttaatactg
    44881 cttctaattg ctccaagttc ttaactttaa ctctaatctt aggagctgta tcaagttttt
    44941 tctgtttatt tactctattt ggctggtatt ttaatctatt cttcttaaat tttctatctt
    45001 ttatctttat tctttcttca tctaaaagct caatacaatc ccttctcatc tgatttaaca
    45061 cacttatagg catacttaca ccttcatcca ggtcaatttc tatctctttt aacttatatg
    45121 gtgtatttcc aagcttttga atttgtgttt ctattttttc tttacttaaa gccaccttta
    45181 tagctttttc aactattttg tctccttcta ccaccgattt atttccacaa aaatcactta
    45241 taaaaagctc tggcttttta cctagtttaa tactcacttt tgcacgtata ttagttttta
    45301 tgaactcttt atcttgctga aatgtagctt gtactctatc cattaattcg ctgtcagaag
    45361 tcttatatat aagctgattt ctctttgctt ctccaacaaa atctaattct atcaaatccc
    45421 ctttatgtgc tattgtagtt atctctccat cctttattat tcttccaata gtacctccac
    45481 ctaagttaat tccatcacct ttcttaagcg tattttctag cattactcta agtcttttgt
    45541 tctttctatt ataatctaaa acttttccaa tatatagccc ttgattatta ggtctattag
    45601 agttcattat atccttacca acttcaccta atatatatcc ttttgtaaac tttctattaa
    45661 atatggtata caaattattt attatttcct cagaaatact tatttttttc ttatctacaa
    45721 attcattaat cgcttctcta tatcctgaaa ccacagtagc tacatactct ggctttttca
    45781 ttctaccttc aatttttaaa gataatacac ctgcttcaat tattttatcc aaatcttcta
    45841 tagtacttaa atctctagga ctcaacaaat aatctccatt actttttact acttctcctg
    45901 tatttatatc tatcaactca tacttttgtc tacatggctg tgcacaccta cctctatttc
    45961 cagacctatt gcctatcata ctactcataa ggcattgacc cgagtaacat acacataaag
    46021 ccccatgtac aaaaacttct atatctacag ttgtattttc acaaatccat tttatctcat
    46081 caacatttaa ttctcttgct aaaacaactc tatcaaaccc tacactctga aggtatttta
    46141 catcttctaa ggaatgtgct accatttgag tgcttgcatg tagttcaaag tcaggtaata
    46201 gtttttttat aagtcttgcc attcctatat cctgtactat taaagcatca atatctatat
    46261 cgtataaaaa ttttacatac tctacaaaat cttctatttc atactgtttt atcaatgtat
    46321 ttacagtaac aaagacttgc actcccctta tatgagagta tttaacagcc tcaatcagct
    46381 catttctatc aaagttattt gcagatgctc tagcactaaa gtctttacca cctaaataca
    46441 cagcatttgc tccgttttga actgctgctt ttaatgaatc aaaagagcct actggtgcaa
    46501 gtaattctat ttttttcata tttttctcct ctatttatac ttgttaggtt aatataatta
    46561 tatctataac ttaaatattt gtattaaatt tatatatttc ctactatatt ttaaattctt
    46621 caatcaattt atatattagt tatttatatt tggttcattt gagttaacat atacattatt
    46681 tactatattt agtatatttc tattaactta tatattgatt actgta
//
LOCUS       DAEORZ010000013        46358 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEORZ010000013 DAEORZ010000000
VERSION     DAEORZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.013"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..751
                     /locus_tag="KRH39_000662"
     CDS             <1..751
                     /locus_tag="KRH39_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBG8510586.1"
                     /translation="MIIPKEQQFEINSSKNMVKDFQGRIYNIDTPVIFVCSIFEGLEK
                     FDIQLALRNKLLIDGYKVLQIGTRQVCELFDFYSFPEFMYSNNIEEQDKVLMLNNTLK
                     KLELEKKPDLIIIGIPGGILPFSSKAFGDFGITMYKVLQAVTPDCTILSIPYGKYSDE
                     EISYFGKITKSKFNIEVDYYNIAAKSILSHPTETMGKPFYLTLGKSLVEKKIATLQNK
                     NVFCLSNKSEIDRLIELMIKEFIKYGTVASI"
     gene            785..1075
                     /locus_tag="KRH39_000663"
     CDS             785..1075
                     /locus_tag="KRH39_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HBG8510587.1"
                     /translation="MVNTEVMNKLDIYTVLVNIFEKRSKINFDENCYLKNENLFESKI
                     MMPARELVLTLYDIEDYFSISIPEDVILSGRFNSFNNIYDIVKKLKNSINFN"
     gene            1117..1335
                     /locus_tag="KRH39_000664"
     CDS             1117..1335
                     /locus_tag="KRH39_000664"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510588.1"
                     /translation="MSNFGKRNLYDSANGEIDPKGGCNCGALCANLCSCSCSGGTWSF
                     YHTNQVKGSKINNGQQFRSPGEIPGQKK"
     gene            complement(1539..3206)
                     /locus_tag="KRH39_000665"
     CDS             complement(1539..3206)
                     /locus_tag="KRH39_000665"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBG8510589.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMNDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNSY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(3403..4125)
                     /locus_tag="KRH39_000666"
     CDS             complement(3403..4125)
                     /locus_tag="KRH39_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8510590.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            4516..5943
                     /locus_tag="KRH39_000667"
     CDS             4516..5943
                     /locus_tag="KRH39_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG8510591.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            5936..6715
                     /locus_tag="KRH39_000668"
     CDS             5936..6715
                     /locus_tag="KRH39_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBG8510592.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            6978..7703
                     /locus_tag="KRH39_000669"
     CDS             6978..7703
                     /locus_tag="KRH39_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8510593.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNENKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            7833..9758
                     /gene="mngA"
                     /locus_tag="KRH39_000670"
     CDS             7833..9758
                     /gene="mngA"
                     /locus_tag="KRH39_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBG8510594.1"
                     /translation="MEFKKITNENLVFLKQKIKTKKDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEASFAAMTLEIPIENWESVDPNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFGIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            9902..12517
                     /gene="mngB"
                     /locus_tag="KRH39_000671"
     CDS             9902..12517
                     /gene="mngB"
                     /locus_tag="KRH39_000671"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG8510595.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSTQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            12777..13931
                     /locus_tag="KRH39_000672"
     CDS             12777..13931
                     /locus_tag="KRH39_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBG8510596.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEKLDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(14054..15967)
                     /locus_tag="KRH39_000673"
     CDS             complement(14054..15967)
                     /locus_tag="KRH39_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8510597.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIAKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNITLTSVELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            16209..16526
                     /locus_tag="KRH39_000674"
     CDS             16209..16526
                     /locus_tag="KRH39_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8510598.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            16534..17781
                     /locus_tag="KRH39_000675"
     CDS             16534..17781
                     /locus_tag="KRH39_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8510599.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            17810..18163
                     /locus_tag="KRH39_000676"
     CDS             17810..18163
                     /locus_tag="KRH39_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG8510600.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            18185..19630
                     /locus_tag="KRH39_000677"
     CDS             18185..19630
                     /locus_tag="KRH39_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG8510601.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDKNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEEARYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKKRYKKDSFYWYQNLIRTNGEELKEK"
     gene            20022..20774
                     /locus_tag="KRH39_000678"
     CDS             20022..20774
                     /locus_tag="KRH39_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8510602.1"
                     /translation="MIIEINKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            20876..21796
                     /locus_tag="KRH39_000679"
     CDS             20876..21796
                     /locus_tag="KRH39_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBG8510603.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPCLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            21799..23715
                     /locus_tag="KRH39_000680"
     CDS             21799..23715
                     /locus_tag="KRH39_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBG8510604.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGSLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMIAFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKKGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            23735..24604
                     /gene="gatY"
                     /locus_tag="KRH39_000681"
     CDS             23735..24604
                     /gene="gatY"
                     /locus_tag="KRH39_000681"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG8510605.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            25114..26577
                     /locus_tag="KRH39_000682"
     CDS             25114..26577
                     /locus_tag="KRH39_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBG8510606.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIAVGLEELERMLEV"
     gene            26581..27681
                     /locus_tag="KRH39_000683"
     CDS             26581..27681
                     /locus_tag="KRH39_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBG8510607.1"
                     /translation="MEYIKLNELCYINIGKTPSRNTSDYWGSGNRWLSISDLKEKYIS
                     KSKEEITDLAVEKANMKLVPKNTVVMSFKLSIGRVAILKEDMFTNEAIANFQIKNNEL
                     ITSEYLYYALRTLNFNNTDRAVMGATLNKSKLNDIKIPYFTICIQNKMVKVLNKAQEL
                     INKRKEQIEALDELVKSRFIEMFGNVITNSKDWDTELLGEISNLKAGKNIKAKDIYEK
                     NSHELYPCYGGNGLRGYVKMYSHKGTFNLIGRQGALCGNVKYVNGKFYATEHAVVVQP
                     KVDINSYWLYFTLKELDLNRLSTGAAQPGLTVGKLNEVEIPKVPVYLQNKFVDFVRQI
                     DKLKSRMEDSLKELENNFNSLMQKAFKGELFN"
     gene            27714..28022
                     /locus_tag="KRH39_000684"
     CDS             27714..28022
                     /locus_tag="KRH39_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510608.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            28055..28435
                     /locus_tag="KRH39_000685"
     CDS             28055..28435
                     /locus_tag="KRH39_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBG8510609.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTKALRNILDIDVDLKIVTDNYTIALKKAILEGKVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            28425..28853
                     /locus_tag="KRH39_000686"
     CDS             28425..28853
                     /locus_tag="KRH39_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510610.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDHLLTCEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            28903..32238
                     /locus_tag="KRH39_000687"
     CDS             28903..32238
                     /locus_tag="KRH39_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG8510611.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYTNDSDLLLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTANYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLTSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKTETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            32619..34682
                     /locus_tag="KRH39_000688"
     CDS             32619..34682
                     /locus_tag="KRH39_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBG8510612.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(34899..35465)
                     /locus_tag="KRH39_000689"
     CDS             complement(34899..35465)
                     /locus_tag="KRH39_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG8510613.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            35631..36152
                     /locus_tag="KRH39_000690"
     CDS             35631..36152
                     /locus_tag="KRH39_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG8510614.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGVFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
     gene            36951..39029
                     /locus_tag="KRH39_000691"
     CDS             36951..39029
                     /locus_tag="KRH39_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBG8510615.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            39029..39808
                     /locus_tag="KRH39_000692"
     CDS             39029..39808
                     /locus_tag="KRH39_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8510616.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            39801..40610
                     /locus_tag="KRH39_000693"
     CDS             39801..40610
                     /locus_tag="KRH39_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG8510617.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            40630..41112
                     /locus_tag="KRH39_000694"
     CDS             40630..41112
                     /locus_tag="KRH39_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8510618.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            41133..41537
                     /locus_tag="KRH39_000695"
     CDS             41133..41537
                     /locus_tag="KRH39_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8510619.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(41693..42853)
                     /locus_tag="KRH39_000696"
     CDS             complement(41693..42853)
                     /locus_tag="KRH39_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG8510620.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            43061..44611
                     /gene="xylB"
                     /locus_tag="KRH39_000697"
     CDS             43061..44611
                     /gene="xylB"
                     /locus_tag="KRH39_000697"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBG8510621.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKIND"
     gene            44707..46044
                     /gene="xylA"
                     /locus_tag="KRH39_000698"
     CDS             44707..46044
                     /gene="xylA"
                     /locus_tag="KRH39_000698"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBG8510622.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
BASE COUNT        18085 a         5060 c         8235 g        14978 t
ORIGIN      
        1 aatgattata cctaaagaac aacagtttga aattaatagt tcaaaaaata tggtaaaaga
       61 ttttcaagga agaatataca atattgatac acctgttatt tttgtatgta gcatatttga
      121 aggcttagaa aaatttgata ttcaattagc attaagaaat aagttactaa ttgatggata
      181 taaggttctt caaattggaa cacgtcaagt ttgcgaatta tttgatttct actcattccc
      241 tgagtttatg tactcaaata atatagaaga acaagacaag gtactaatgc ttaataatac
      301 tctaaaaaaa ttagaattag aaaaaaaacc tgaccttata attatcggaa ttccaggtgg
      361 tatacttcca ttttcttcga aagcatttgg tgattttgga attacaatgt acaaagtact
      421 tcaagcagta actccagact gtacaatact atctattcca tatggtaaat atagtgatga
      481 agaaatttct tattttggta aaattacaaa aagtaagttt aatatagagg tagattatta
      541 caatattgct gcgaaatcaa tattatctca cccaacagag actatgggaa aaccatttta
      601 tttaacatta ggaaaatcat tagtcgagaa aaaaatagcc acattacaga ataaaaatgt
      661 attttgttta tctaacaaat ctgaaataga cagattaata gaattaatga tcaaagaatt
      721 tataaaatat ggtactgtag catctatata aaatatttta cataaaggat ggtgaaatac
      781 tgatatggta aacacagaag ttatgaataa actagatatc tatacagttt tagtaaatat
      841 atttgagaaa agatcaaaaa taaattttga tgaaaattgt tatttaaaaa atgaaaattt
      901 atttgaatct aagataatga tgcctgctcg tgaactagta ttaacattgt atgatataga
      961 agactatttt tctatatcta ttccagagga tgtaatttta tcaggaagat ttaattcttt
     1021 taacaatatt tatgatattg ttaaaaaact taagaattct attaatttta attaattaaa
     1081 atataataat ataacaaatg agaggggaaa aatgatatgt ctaattttgg aaaaagaaat
     1141 ttatatgatt ctgcaaatgg agaaatagat cctaaaggag gatgtaattg tggtgctctg
     1201 tgtgcaaatc tatgctcttg tagttgttct gggggaacat ggtcattcta tcacacaaat
     1261 caagtgaaag gttctaaaat aaataatggt caacaattta gatcaccagg agaaatacct
     1321 ggacaaaaaa aataaaaaga gttaaatata attagtttta gctttatcaa ttaacataat
     1381 taactttatt aaaattaata gaatctaatt acaattgttt attatatcta taaaagcaac
     1441 tttaaaatac cttgttttat atatgttaat taaaaatggt aggagctgtc gtgctagaaa
     1501 atagtgctac agctcctacc atttttacat aaataacttt atttataata tatcattata
     1561 ctttcgtaag gttttatttc cattttattt tcaattatat ctctagtgta attggtttca
     1621 agtattgtgt agtttaattt attcaaataa gtttcaggta atttatatac tacattatca
     1681 tgagtaaaat tacttataac aagaagattt tggtttttat actctctttc ataaacaaat
     1741 agatttttat gattttcatc taacaatcta taagtacctt ctgtaaaaat atcatattgt
     1801 ttacgatatt tgattaatct ttgataagta taaaatactg aacgagaatc attaagctca
     1861 ttttctacac taatattgtc attattaact tttaaccacg gtttatgatt agaaaacccc
     1921 ttataaatag tattatccca ttgcatagga gtacgggcat tatctctgga tttttgttga
     1981 agtataagca atgcctcctt ttcatcaata ccagatttaa tcatatgata gtatgaattc
     2041 ttactttcag catcatcata ttcatctatt ttagtaaagt aggcatttgt catacctatt
     2101 tcttcacctt gatatatgta tgtagtacct tgtagaccaa atagtgttat ggctaacatc
     2161 ttagcacttt cttgacgaaa aaatctatca tctccaaatc gagataaagc tctaggttgg
     2221 tcgtgattag agtaaaatag agcattccat ccctttcctt catacatttt atattgaaag
     2281 tcagattgtg ctttttttaa tttatccaga gagaacattt cattagtcca ttttgcgcct
     2341 cctttataat ctgttttcat atgatggaag ctaaaaacca tagatagttc ttctcgattt
     2401 tctctagtat agttgataca ctcttcaggt gtagtagatg acatttcacc tacagtaact
     2461 atatttttag gtttgaaaac tttttgattc atattatgta aatattcgtg tatacgagga
     2521 ccatcagcgt agtgagttct tccatctcct tttggtcctg ttatagggtc atcaggtaat
     2581 ccaggtgttt tggagagtag gttgactaca tctaaacgaa aaccatcaat acctttatca
     2641 gcccaaaatt ctagtatttt gtaacattct tcacgaacct tttcattttc ccaatttaaa
     2701 tcagcttgag tttcttcaaa taaatgtaaa taatattggt tagtggtttc atcatatttc
     2761 caagcagttc cactaaatct ggatatccag ttattaggaa cagaaccatc aggtttagca
     2821 tctttccaga aataataatc acgatatgga ttgtctttgc tttttttaga ttctttaaac
     2881 catttatgtt cagtagatgt atggtttagg accatatcca tcattatttt tatgtctctt
     2941 ttatgagctt cttttaagag tttttcaaag tcattcattg tcccatattt tgggtctata
     3001 ttataataat cttctatatc atatccatta tctctttgag gagaaacgta cataggagtc
     3061 aaccataata aatcaacacc taaagaatac agataatcta atttttctat aataccattt
     3121 atatcaccta taccatcatt gttgctatct ttgaaacttt ttggatatat ttgatagaca
     3181 gttgcttttt tccaccaatt tttcatactt gatacctcct gtaatttata agttaattat
     3241 aatatgttta aacaaattgt caagcgttaa actaatatta aagctaaaat atacaataaa
     3301 taattatttg tataaacata taattgtata tagaatagtg aggttagtat gggataaagt
     3361 gtctaaacgt aaaaaaatta tgtttaatta aacataattt ttttaatatc tttgagtaac
     3421 atccatgaat acaaatgttt caggtgtatg ctttgattca gtatattcaa aaagctttcc
     3481 atcatcagta tatacactat ttttaataac accaacacaa ttataatcat ttagaggtag
     3541 cgtttttaac tcaagctctg tagctttttc aattttaaat atctttctgg atgaaacaat
     3601 ttttgtacct agacactctt ctatatattt ataaatagag ccttgtgcta tagaaacatc
     3661 aaggtcttta actatactac atagaaaata gttaatgtcg agaattttag gtatgttatc
     3721 aatatatctt agtctgtgaa ggtaatatac ttcactacca actttaaaac ctgtcttttt
     3781 agataactta ttatcaatta agattttgct gaagtggaga acactagtag cagtattaac
     3841 ctttttatct tctacaatcg attctacacc tttgaaattg tgcatattta gaaaaaaatc
     3901 attagaacaa ctattttcta acactacaac acccttgcct tttatacttt gaacatatcc
     3961 ttctgtgttt aattgagaaa ttgctctacg tacagtgttt ctgctacatg aaaacctttc
     4021 aataagttga agttcagtag gcagcatcat attacttgta tatttaccat caagtatttc
     4081 ctctttcaat acttcatata tctctgaaaa tttattttta gccatatacc tctatccctc
     4141 cttaaaggtc tttagtaata ttattgaact gatatgaaca aattatatca aatgtgttta
     4201 gacatgtata gtgaaaatag aatcatatca accataaaaa ggacaaaata gaattatttt
     4261 gtgggttgtt tcaaatacat ggttaaacat atttttaaaa taaaaaaaat gaattaaaaa
     4321 aataggctag atatagcaat acacttaaaa ggatttatat gttaaactaa atgataaaaa
     4381 taaaatgaat aaaaaatatt gacaatttgt ttaaacaaat ataatattta aaatataaaa
     4441 tgtgaataaa tcgttttcac aaaaaattaa accgaaatag aaaggggatt gtatatgaag
     4501 aggttcttaa acaaaatgat gcaacaactg tcacttttag gaaagactat gctggtgcca
     4561 atagctgtaa tgccagcagc aggtatatta ggattattat ttggtccaaa tatgctcaat
     4621 gtgccagcta tatctaatat atctaatata gtattttcaa atgtggacta cttattttta
     4681 ttaggagcag ctagtgccta ctcaaagtct aaagacaaga caagtgtaat ggttgcagcc
     4741 gtagttgcat atatgatatt taagtcaaat ttagagttat tgaatgatac attaaatgca
     4801 ggtgtatttg gtgggataat tgttggagct tgtatagcat ggacttacaa taaaacatat
     4861 aaagttaaag ttccagcatt tttaagcttc tttgcaggag aaaaatgtgt aattacacta
     4921 tcaccagtgg ttgctttaat tttatcatat atatttagtt tattatggat atatccacaa
     4981 gctgcaatga atcaatttgg tttttggttg ggaggagctg gtgcaatagg aatattcttg
     5041 tttttattct gtaaccgtgc actaatacca actgggttac atcaagtttt aaatgcttac
     5101 atattatttg aaatgggtga atatacaaca tcaacaggag aagttataag aggagaaatt
     5161 cctagattta tggcaggaga cccaaatgca ggattcttct ggacaggatt ttacataatt
     5221 atgatgtttt caatacctgc tatttcttat gctatatata aaacagcaca agataaaaag
     5281 aagcaagaag taaaaggtgc aatgaccgct ggagcattga cttcttttat ggcaacagta
     5341 actgaaccaa tagaattttc gtttttattt gcatctccaa agttatatat aattcattct
     5401 ttttattctg gtctaggtgg agttgtacta tatttactgg gtgctcgctt gggttcattt
     5461 aatggagcaa gtataattga ctatgtctta agttttagtt atggagataa agcgtggtta
     5521 gttatacctg taggagttgt gttctttgta ttatattatg taacatttaa atatataata
     5581 ataaaagata atgtgcaaac accaggacgt gaagttgaga tagagatagg tgataaagtt
     5641 agtgaaaaag aaaagaattt aaaactttct catggtaatt tttcatatat ggctaaacag
     5701 ataattaaaa attgtggtgg ttatgaaaat atagtgactc ttaataattg tatgacgaga
     5761 ttacgtttag aagtgaagga tgccactatt ttgaatgatg ataatattaa aaaaacagga
     5821 gccaaagggg taattaaatt gagtaataca tcagttcaaa tcatcatagg aacggatgtt
     5881 gttaaagtaa aggatgagat ggaaatgcaa cttgatgagt tgagaaagca ggcagatgca
     5941 taaaatgaag aaattagtag ttagggcaga tgatatagga tattcagaag gtgttacact
     6001 agggataata tcttcttata agaacggtat tgttacaagt gctggaatca tggtgaatat
     6061 gcctttttca agacaagcta tagaaatggt aaaaaatgat gatgtatgtt ttggtttaca
     6121 tgttaattta gtaataggag aaccttgttg caaagaacat attaagataa agaatttgtt
     6181 agatgaaggt ggaaaatttg tttctagcaa aataagaaga acgcaactac tgacaggtaa
     6241 agaggttttt aattatgaag aagtatatga agaagtttgt gctcaaattg aaacttacaa
     6301 gttgtatatg agtaaattgc ctgagtatat tgatatacat ggtatagaag aagatgtatc
     6361 aatatcagca atgtgtgatg ctgcaaaaga atacggtata ccaagttgtc cctattattt
     6421 ggattcatgt attagcatgc caagtaaata tcctcagaat gatttttaca agttaaataa
     6481 atcttttgtt aaattatttg aagaaaaata tataaattta gataaagata taacattgat
     6541 ggttactcat ccaggattta tagattatag tcttataaaa tcatcttctc ttattcaaga
     6601 gagagtatat gaacatgcaa tgctatgtga taaggaagtt aaaatgtggt taaaaagaaa
     6661 taatattgaa ttggttgatt ttaaagatat tttcaagtct aataattata tataaagaat
     6721 agattaccta aagttttaat taaaaataag tatttgaaaa aaatgtgtat tttctaaaaa
     6781 aatattgata gagatgtatc aaaataaaaa cttgaatcat ctctatcaat ataggaagta
     6841 cacatttttt atgtataata aaaagagtag tatgcattaa taaattaaaa atattaaatt
     6901 ataattatat taacgaaaat tttagctaat atttttgaat tttacttatg aataataaaa
     6961 tagatggagg agaaactatg gaattttatg aaagagcaaa aaaatatgag tataaattta
     7021 cagatacaga agacatgata attgaatata tgagaaaaaa caaagaagaa atagtaaaat
     7081 tatcaataca atctttagca gataaatttt ttactgtccc aaataccata gttaggctat
     7141 gtaaaaaaat ggagtatgat ggtttctctc aactaaaaaa taatttaaaa gaagagttaa
     7201 aaaatgagaa taaagagcca aaaaatggtt ttgaatcaga tataaaaaag acattagagc
     7261 taatagatta tgaaaaaata aataaagtag cagatataat tcaaaaatct aggagtatta
     7321 tagtttatgg aataggtcaa aacattcctt tatgtgagat attagtaaaa gaaattagag
     7381 gtgggcatga taatataact tactatagtc aaagacatga agtacttcat gatataggaa
     7441 aattaggtga acaagatatg ttttttgcac ttagtttaag tggagaaacg aaagaagtta
     7501 tagaagcagc aaagacagca gttaataagg gttgtaaggt agtagctcta acacatatat
     7561 gtgaaaatca attgcaacaa attgcagata taagtttata ttgctattca acaattaaac
     7621 aagtaaatgg gtacaatata tcagataagg ttccaattat gatgattatg agagcaataa
     7681 tagagcaata ctggttaaga tagagatttg tgtataaata cacaaatctt tattttttaa
     7741 gaagaaattg tgtgtataat ttcaaaaaaa cattttcata atttatttta tgctatattc
     7801 atagcattaa atgatttaag gagggcgtaa ttttggaatt taagaaaatc acaaatgaaa
     7861 accttgtttt tttaaaacaa aaaataaaaa caaagaaaga cgcaataaag ttcttagcaa
     7921 gaaagctata tgatgaaggc aagttaacat cttatgagga atatttagaa gctgtaatgt
     7981 acagagaaac tttatctcca acaggttttg aaagagggct tgcaattcct catggaaaat
     8041 ctaagtcagt taaagaagct tcatttgcag ctatgacctt agagatacca atagaaaatt
     8101 gggaaagcgt agacccaaat aataaagtag agctaatatt tttaatagca gtaccagaaa
     8161 gtgaaggtgg aaatacacac atacaaatac tttctgagtt agttactaga ttgtcaaatg
     8221 aagattatat gaatatgttg ttaaattcaa aaactaagaa agagttattt aataacttag
     8281 atataaaaga aaagaaagaa caaataaaag aagagattag gattcaaaat gaatcaaaaa
     8341 aaattatatt agcaataaca gcatgccctg caggtatagc acatacatat atgtcagcag
     8401 aagcattaat taaagctggt aaagaaatcg gtgtggatgt gtatgtagaa aagcaaggag
     8461 ctaatggtat ggtggaccct catacaccag atataataaa aagggcagat gcaataatat
     8521 atgcaacaga tgtagcacct aaaaatacag aaagatttga acatttgcca aacataaaaa
     8581 cgtcagtagc agctccacta aaaagagcaa aagaaataat atacgaagct ttagaagtag
     8641 cacaaaaaca aggtaaaggt gattatatag aaaaatcatc tgatatatca tattgtgaaa
     8701 aatcatcatg gaaaaaagat gtaaaatcag caatacttac aggagtatct catataatac
     8761 cacttattgt ggcaggaggt atgatacttg catttgcagt attagcatct caagcatttg
     8821 gtctacaaga actatacaat caagaaggtt cttggttatg gcaattaaga aagctttctg
     8881 gaggtatgtt aggtacattg atggttccag tattatcagc atatatgtct tactcactag
     8941 ctgaaaaacc aggtcttgga ccaggatttg cagcaggtct agcagctgat ttaataggta
     9001 gtggatttct tggaggtatg ttaggaggtt tacttgctgg ttatatagta aaatttttaa
     9061 agaagaatat accagccaaa ggcacattag ctggattcgt aagtttttgg gtatatccag
     9121 tagttggttc tttggtagta ggtttattga tgttatttgt agtaggaaag cctgtagcag
     9181 cacttaacaa tggtcttata aattggttaa atgggatgac tggagtaaat gcaatactac
     9241 taggagcaat aataggagct atggtttcat ttgacttagg tggaccaata aataaagcag
     9301 cctatacatt tggaatagga gcaatagcaa gtggaaattt tataccttat gcaatatttg
     9361 catcagttaa gatggtttca gcttttggag ttacaatagc tactgtttta ggcagagatt
     9421 tattttcaga atcagaaaag gaaataggaa agcaaacttg gatattagga ttggctggaa
     9481 taactgaagg tgcaatacca tttatgatag aggacccaat ccgtgttata ccatcattgg
     9541 tagctggttc agccataact gggtctatag tagcattctt caatataggt cttcaagtgc
     9601 ctggagcggg gatattctca ttggcgcttt tagagggaag tatgtcagga tttattatag
     9661 catcaatatg gttctttgcg gcagtacttg gagctataat atccgcaata ttattaataa
     9721 taactaggaa acaaaaaaat caaagaaaga tacattaaaa gatacgttat tagatattca
     9781 aatcaaaggt ttaattatag tgataaaata aatattaaaa aattttgtaa actcagtagt
     9841 atttttattg gatttttaat tagacagaaa atattatcag ataagttttg gaggataagc
     9901 aatgaaaaaa aatgtacata tagttcctca tatgcattgg gatagagaat ggtacttctc
     9961 aactgaggaa tctagaatat tacttgtaaa taatatggaa gaaataatgg atatgttaga
    10021 aaataatcat aactatccat attatgtgat ggatggtcaa actgctatat tagaagatta
    10081 tttagcagtt aagcctgaat gtaaggagag aataaaaaaa ttagttcaag aaggaaggct
    10141 aataataggt ccttggtata ctcaaactga tgaaatggta gttggtggag aatccatact
    10201 tagaaattta ctatatggaa taaaagattg tgatgaattt ggagaatata tgaaaatagg
    10261 atatttacca gattcatttg gtcaaagtgc tcaaatgcca caaatactaa atgggtttga
    10321 tataaaatac tcaatgtttt ggagaggatg ttctgaaaga aaaggaacta ataaaacaga
    10381 gtttaattgg aagagtgatg atgggtcttc tgttttggtt caaatacttc cattaggtta
    10441 tgcaatagga aaatatctac caatgaatga agatgagcta agaacaagaa tggataaata
    10501 tttaccggtt cttgataagg gtgcaactac tgaccatata atacttccaa atggacatga
    10561 ccaaatgcca attcaaaaga atatatttga agttatatat aaattaaaag aatgttatcc
    10621 agagagaaaa tttttcttaa gtagatatga aaacatattt aaagagctag aaaaaaatga
    10681 ggatatagat acaataaaag gggaattttt agatgggaaa tatatgaggg ttcatagaag
    10741 tatattctca acaagaatgg atataaaatc agcaaatgct agaatagaat caaagataac
    10801 taacatattg gaaccactag cttctattgc atattctcta ggatttgaat atcatcatgg
    10861 attaatagag ttaatttgga aagaaataat gaaaaatcat gctcatgaca gcattggatg
    10921 ttgttgttca gataaagttc accatgaaat aatgaataga tttttcttag ctgaggaaaa
    10981 agtagaccaa ttaataacat tttacaaaag aagaatagtt gatgcaatga gttgtgaaat
    11041 ggctttagat aagttagtag catttaactt aatgccttac gataggaatg aaatagtgag
    11101 cacccaagtt ataactaaaa tgaaagcatt tgaaatattt gataaagata ataataagtt
    11161 ggattttgaa gtggtacata aagaagtaat agatgcagga ttaatagacc gtcaaattgt
    11221 tcattatgga aactatgatt catttatggt atatacaata aactttaaag ataaaatacc
    11281 tgcaatgggg tataaggcgt atatgataaa agaagttgaa catatgttag aaaaagctta
    11341 tgaagtatgt gacatggtag ataatgactt ttatactatt caagttaatg aaaacgggac
    11401 attaaagata tttgataaaa aattaaataa aacttttgaa aatgtattat tgatggaaaa
    11461 tggaggagat gaaggagatg agtatgattt ttcaccactt ccaaatgaga aattaatatt
    11521 taacgataat atagtagcaa attccactat aaagaaaaat agatttaata atgagataaa
    11581 gataaattat agattaaaag taccaaaaga tatacaagct agaaaaaata atagagcaga
    11641 ttcatttatt gactttgata tagttataaa tgttccaaat gataaagcta taatagatgt
    11701 agtattcaac ataaataacc aagcaagaga ttgtagggtt agaacttata taccaacaaa
    11761 tatagcttca aagttttctg tgtctgataa tcagtttgga catataaaga gaagtgtgta
    11821 tgatgatgct atggaagttt ggcaacagga agaatggagt gaaagaccag attctatata
    11881 tccaatgcta acattcgttg gattgtctga caaagagcat ggagtagctg ttcttacaaa
    11941 cagtacaaga gagtttgaaa tagtagggga aaaatttgac acaatagcta taacattatt
    12001 tagaagtgta ggattcctag gtaaagaaga aatggcaaga cgcccaggtc gtccatcagg
    12061 aataaagttg cctactccag attctcaaat gataggagat ataaaaatag acttagcaat
    12121 agttactcat gaaaaatcta cactagaagc aaatgttgca aatatggcta aagagtattt
    12181 aacaaaaata caaacttata ataaaatgcc atataatgct atgaaattga atcaatcaga
    12241 aatagaagta gattattcat atagtttact taaggaaact aataaggacc ttgtgttaag
    12301 tgttgtaaaa aaggctgaaa aagaagaggc attagtagtt agattttaca attctaaaga
    12361 agaaacaaaa tcagctaaca tagtatttaa caaaaatatt aaaacagcta aatggattaa
    12421 cctaaatgaa aaagaattag aatctatatc aatagataat aactctgtaa atgtagagtg
    12481 cttaatgaac caagttaaaa ctatactggt taaatagtgc taaaaaggaa ttgtcttaaa
    12541 atagagataa tcctccttat cattgttgaa ataataaaca tatatttgat tttatacaaa
    12601 aagagtgtct catgaactaa gaaatttatt ttgagacact ccctttttat aagataaaag
    12661 aatattaatg gtataagttg tatacaaaga gagatgtaga gtataaaata aaagataaat
    12721 taagcttata aaattatatg tttaaaataa ggagtaataa aaaggagtag ataagtatga
    12781 aaatattaat ttctatagat tctttgaaag gaagcttatc ttcaatagag gcaggaaatg
    12841 caataaaaaa ggggatattg aaggttaaag aagatgcgca agtaaagata ttgccacttg
    12901 ctgatggtgg tgaagggaca gtagatgcct tagttcaagg gatgaatggt aaaaaagaga
    12961 ctatagaagt tactggacca atagctaaaa aagtggatgc aacatatgga ttattaaaaa
    13021 atacaagtac agcaataata gaaatagctc aagcatcagg actaacattg gttccgactg
    13081 aacttagaaa tcctttatac actacaactt atggtgttgg ggaaataata aaagaagcaa
    13141 taaataaagg ctatagaaat tttatagttg gaataggtgg aagtgctact aatgatgctg
    13201 ggataggtat gcttcaagcc ttagggtttg agttttatga tgaaaataac aagttagtag
    13261 gattaggtgg aaaagtttta aatgaaataa gacatataaa aatagaaaat agattaaagg
    13321 aattagatga atgtaagttt aaaatagcct gtgatgtaaa taatccgctg tttgggaaaa
    13381 atggagctgc atacatatac gggtctcaaa aaggtgcaac atcagaaata attgagaagt
    13441 tagataatgg gcttagaaat ttttccaaag tagttaaaaa ttacttaagt aaagatgtag
    13501 ctaatgtaga aggagcagga gcagcaggag gattaggttt tgctttttta gcatttttaa
    13561 attcaaaatt agaatctgga ataaaaataa tattagaaga aattaaatta gaggaagagt
    13621 taaaagatgc agattttgtc ataactggtg aaggtagatt ggacaatcaa actgctatgg
    13681 gcaaggctcc aataggagtt gcaaaactgg caaaaaaata tggggtaaaa gtaataggtt
    13741 tggctggagc cacaacagaa gatgcagtta aatgtaatga agagggtata gatgcttatt
    13801 tttctatagt aaatagagct atgacaatag aagaagcaat ggataaagca actgctagtg
    13861 agaatatgac agctacaaca acacaaatat tcaatcttat aactagtata caacagtcaa
    13921 aaaatatgta gtattaaaac aaggtatatt aggtaaaaag ctgtcacata ttaatttaga
    13981 ctttttagtg tgacagctta tctattttat gttaataact taagaaacat atattggaaa
    14041 taaattaatg gatttagata ttttgaataa gataagctat tctattcata aaactactat
    14101 agttatctgc cttagtaagt tctttaacat tattccactc agataatatt tcaataaagc
    14161 tttcaaatat gtaattgaat ttttcttttt ctttttcatt tataggtatc atagctataa
    14221 tctgaacttt ttcctcaccc catttaactg gtttatcgtt aacaattaaa catactccag
    14281 tttttaaagc atccattttc atgctatgag gaattgctac attgttatta aatgcagttg
    14341 aagacatttt ttctcttata agaacatcat ctatataatt gggtttgaca tatttatttt
    14401 cgtacagaat atttcccata aatcttatat aatctttagc actatctaaa tatatatttt
    14461 tcataaacaa agattcacta aagtacattt ctaaatgatt ttgtacagta agtatatttt
    14521 ttctatcttt aatctgaata aatttacttt gaatattatc ataatccttt ctatttaggt
    14581 aaggatttac ataaacaaaa ggtatagggc atctatcgct aattggtaaa gtagtaataa
    14641 ccatgtctac cttattgtca aaatcaagat tatgagtatc tgaaaaaatc tgaataattt
    14701 ctatggattc attaaatctc ttctcaaggt ctcttactat tttgaattgt aaatcatagt
    14761 aatttggagt aattaatgca cagagaattt tatcttctag taatttttgt ctatcaaaaa
    14821 atgaacctac atgaagagat atatacgata tttcatcttc atctatttct atgttttcta
    14881 aaatttgaat ctgattacaa atataaacag atatatcata tatcagaggg taggaatctt
    14941 ttaacttctg aggtatttga tttctcaaaa cttgattatt tttagcccta ctgattaaat
    15001 tttttacatg aagagtaaat tttacaacaa attcatcatc agataaatct ataaagtata
    15061 agtcatatac atttttaatt atttttttag ttaatttaac aaaatgttca tctatataat
    15121 ttggcaatga cttagtatcc atttcatgat aatttaagac tgtggttttg tttgttaact
    15181 gaaatactaa gtagtataat tctacactag ttaaagttat attgtattca gactctaaaa
    15241 aatttgatat atctcttgaa atgcttatat cattattatt atctatgtag tttactactt
    15301 cgacagattc tatgtagtta ttctttttta ttctgtctat agttatcatt aaatgaagaa
    15361 gtatgttatt tatagcatac tcatttataa acaaattgta tttttttaat atatatatta
    15421 tattttcttt cagtttggtc aaattatact cttgatatat tttttgtaca tcaaataagc
    15481 ttaaaaaatc acttcccgtt tctttaaata ttatagagct cattaatttg cgctttgcag
    15541 actcatttcc aattagtttc aataaaaatt tcgattgctt tatctttaaa ttataacttc
    15601 ctataaattt attagctttg gctatatctc cttcaatagt agctggacta acaaacaatt
    15661 cttcacttaa atcaaatata ttaattccat tactattaac tataagttct tttaaaatta
    15721 aatttaacct gttatcaggt gatatatttt ctgaactatc aaccttggtt tgacaattat
    15781 tattgagatt tactttatat ccttttttag aggataatat aaattcacca ttggaattaa
    15841 tttcgtttac atattttcta attgaacgcg ttgagacatt aagataatta gccaatgtag
    15901 atgaatctat ccaatcatgt tgtgttttta tgaaatttaa aaatagttct tttttagatg
    15961 ttttcattag attccctcgc atagttttaa aataaaatct ggatgatatg ataattatat
    16021 aattgaatgt acgtattata agatacatta tactatatta atatgtttaa ttaagagtaa
    16081 ctgatttcct attattagga aatatattta tgtgaaatga acttatttgg taagtacaat
    16141 ataaacataa taaaataaaa agttcattaa gaaataaatt aatggataaa aatattggag
    16201 gtattattat gaaaaatata agagtgcttt tggcgtgtgg ttcaggagca agtacaggtt
    16261 ttatggcaac tagtatgaga aagagtgcaa agaaaaaagg tatagaagca gatataaaag
    16321 cgagaagtga gtcagagata gataagtatt tatcagaaat agattgttta ttactaggac
    16381 cacatttaga ttatatgaag gaagaaattc aacaaaaggc taatgaatat aacgttccag
    16441 tagcatgtat atctaaaact gcatatggaa gactagatgg tgcaaaggct ttggaagaag
    16501 cactagaact aatatcaaac aaataaaata tttatgggga gagtgtttat gaataaattt
    16561 atagattttt taagtgaaaa atttactcct gttgtaaata atatgactaa gaatatatgg
    16621 gtacaatcag ttcaatcaac tataatgaaa gttttgccaa tggtatttgt aggctcatta
    16681 gtaactatag tttctgtctt gaagaattac ataagctttt tacctgattt aagtccaata
    16741 aatcaatata catttggact tttaggatta tttatagcat ttttattgcc tatggaaata
    16801 atgaagaata agaaatttga aagtatgagt gtagtagctg gtcttgcagg cgctggatta
    16861 atgttaatga tgattcgtcc agagattacg aatgaaggag ctatatttaa cttcaacagg
    16921 tttggaggag aaggaatgct tgtatcgcta gttgcaggat tattctctgg attaataatg
    16981 tctttatttg gttctttttc atttttcgga gaagattcag cacttccaga ttttgtaagc
    17041 aagtggtttg ataatatgtt acctataaca gtgataatgt ttgtaggctg gggattaatt
    17101 tatggactca actttgacat gtttgaaata atagttagag tatttaaacc agtatcagat
    17161 atagcacaga cattacctgg atttgtactc ttaaacttct tagtagtatt tatatattct
    17221 ttaggaataa gtgggtgggt actatctcct ataacttatt caattcaact tggtgcaatt
    17281 gcagctaatg cagcagcagt agcatcagga ggagtagcaa ctaatatata tgtttacgaa
    17341 gttattcaaa caggttggat aactttagga gggttgggtg ctacattacc attagttata
    17401 atgatgtgtt tatccagagt tggaagaata aaagcaatag gaagagcatg tattgtccct
    17461 tctataatga atataaatga accagtaata tttggagctc cagtggcatg gaacccattt
    17521 ttaatgttac ctatgtggat aggaacagta gtaatatcaa taactactta cttatcaatg
    17581 agtatgggat ttgtgacaat tccaagtaag gtgtttaatt tatggtatgt tccttttggt
    17641 atatctactt ttatggtaaa tcaagatgtt agagggctta ttgttttagc aataaatata
    17701 gtattgctat ttatgatatg gtatccattt tttaaagctt atgagaaaga agagataaaa
    17761 ttagagagcc aagaggttta aatttagaaa ttgaaaggaa attataaata tgacaattga
    17821 agaacatata atagatgaag aattagtaga agtagcaatg caaataatac ttcgtgcagg
    17881 tgaggctaga acagaaatta aacatgctct taatgatttg gaaaagtttg attatagaaa
    17941 tgccgattta aaactagcta aagcaaaagg gtttatgaca gaagcacata gagctcaaac
    18001 caagattata caaggggaag ctggtggaga gaaaagagca cattcgctgt tatttgcaca
    18061 tgctcaagat actcttatga ccatattcag cgagttaaat ataactatgt ctttagtagg
    18121 tatagtaaag tctatagaat ataaggtaaa taatagtaaa tgatttaaat aaataaaata
    18181 aggagtgatt aaattgggta agtttcctaa aggtttttta tggggaggag ctgtagcagc
    18241 taatcaatta gaaggtgcat ggaatgaagg tggcaaaggt tggtgtttag ctgatgtaca
    18301 tatgtatgat aaagatagag atatatctaa accttatgag tctgatatga ctatagaaag
    18361 aataaagttt gctatgaaag ataaaaatgg gtactatcct aagcgtagtg gaatagactt
    18421 ttatcataca tacaaagaag atttaaagct tttgaaagag ctagggttaa agtgttttag
    18481 attttcaata aattgggcta gaatatttcc aaatggagat gatttagagc ctaatgaaga
    18541 gggattaaag ttttatgata atcttataaa tgaagtaata gataatggta tggaacctat
    18601 tgtaacacta cttcactatg agactccttt gaatttagta ttaaaatata aaggatggtc
    18661 taatagaaaa gtggtagatt tctttgttag atatgcagaa ttaatactaa atagatataa
    18721 aaataaagtt aaatattgga tagttataaa tcaaataaat ttaatacatt atgaatcatt
    18781 taattcagtt gcattttgtg tagaccaagt agataatgta gaagaggcta gatatcaagc
    18841 tattcatcat caattcatag ctacagcttt gactaagaag cttggaaaag aaataaattc
    18901 agatatgatg atagggatga tgatggcaga ttgtacatct tatccagaag attgtgaccc
    18961 tgaaaatgta gtatttgctt taaaaagaaa taggctacaa tattttttca ctgatgtaag
    19021 ttttagggga gagtatccag tctatattaa gagatttttc aaagaaaata acataaatat
    19081 agttatggag gaaggtgatg ataaaatctt aaaagaaaat actatggatt ttctagcttt
    19141 atcatactat tattctacaa cagtatcagc aagtaaaaac accatgaatc caactgacaa
    19201 tataaagaat ccatatttaa aagcaaatcc ttgggggtgg gcaattgacc caaaaggatt
    19261 atacaatact ttatctcaat attatgatag atatcaaaag ccaataatga tagctgaaaa
    19321 tggatttgga atgcatgata aatttgaaga tggaaaagtt catgatagtt acagaataga
    19381 ttatttagga aaacatatag aacaaatagg tgaagctata gctgatggaa ccgaaatatt
    19441 agcttattgt atgtggtcac caatagatat agtaagttgt tcatctcaag aaatggaaaa
    19501 aagatatgga tttatatatg tggatataga taacaatgga aatggaagta aaaaacgtta
    19561 taaaaaagat agtttttatt ggtatcaaaa tttaataaga actaatggag aagaattaaa
    19621 agaaaagtaa atagttttta aatataaaca acccccctta taaatactgt atatattatt
    19681 aatatagttt tataaggggg gttataattg taaataaaat gatttattaa ttatatgatt
    19741 catagcgaaa tttatattta tacttttaat cataaacata tcagtatgag gaatatctat
    19801 tgtacttcat ccaaattttg gtagaggtac aattcttttt tattttaaaa tgctataaga
    19861 taacataata aactttaagc aaaaaattat tatattataa atttacatat atagacattg
    19921 actaaatctt aattgtatat aaaaataagt agttaagagg taatatatta tattactcga
    19981 taaatttaga caaataaaac ttatataagg ggagatagca tatgataatt gagataaaca
    20041 aaagtaattc tgaaaatcta acgaagacgg aacttgaaat aattaaattt ataaatgaaa
    20101 atgaatcaat attaccagaa ttatctatag tagagattgc tcatgaaaca tattcatctc
    20161 cagcaaccgt ttcaagagca attagaaaat gtggtttaaa tggattcaat gaacttaggt
    20221 atagattaac agtaaaagat gaaaatgatg atatacaaaa tatgggggat ataataaata
    20281 aatcctttat agaagcacag agagttatag aacaaatatc tttaactaca ctaatcaata
    20341 taataaaaac tataaataaa tctagtaaga tatatatttt ggcaagaggg cttactgaat
    20401 atgtagcaaa agagtttagt cttaaattgc aattacttga ttttaatgtt gtttttataa
    20461 atgaccccaa cataatgaaa ataaaatcta aatcaataaa aaaagacgaa cttataatta
    20521 tattttcatt gaatggaaat accatagagc ttgtagaatc agcacagaat gccaatttag
    20581 ttggagctaa ggttataact tgctgttgta gtgaaagtgc aaaacttata aagtattcgg
    20641 atttatatat tgtaggatac aaacatgaac atatatcaat ttctgaatat gaagtatctt
    20701 ccagattgcc cttgtatatt atatctagga taattataga ttatatggtc agatataata
    20761 attctgaaaa ttaaattgaa aagtctatga aaatcgattt tctatttgtg aaaagatttt
    20821 catagacttt tttattataa tgaaaacata cacagcacga gcaaggggga agacaatgat
    20881 atatacatta actacgaatc ctgcaataga tatgaatata tctactaatg ggataaagag
    20941 aaaattagta aatcgtacat caaatgctgt atattctcct aatggaaagg gattaaatgt
    21001 aacttttgta ctaggtcatt atggtataga atctaagata ttaggatttt ttggaggatt
    21061 ttcaggaaaa tatatagttg aagaaagcga aaagagagga tttgacgtat tgcctacttg
    21121 ggtagaagat acaactcgta taaatatatt tttaaatgat ggaagtgatg aatttaaatt
    21181 tgtaaactct gggtcatatg taaacgaaaa acaaaaacta gatatgctag ataagataca
    21241 gtcattagaa gatataagtt acttatcaat aagtggaagt ctacctcctg gtattgatga
    21301 taattactat gaggatatat ttaaaatttg taaaaataaa agtataaaga caatactcga
    21361 tataagttca ccaaaattaa aggagttact acagtataat ccatgtttga taaaaccaaa
    21421 tgatgaagaa atcaaagata tatttggaat tattgtaaga gatgaagaag atattaagga
    21481 tgtacttaaa ttactacatt taaaaggtgc tcaaaatata ttgctaactt taggagaaaa
    21541 aggctcttat ttttataatg gaaaatcaat ttattatgct agtgctcaac cagttacagt
    21601 agtcagctct gcatgtgctg gtgattctgc attagctgct tttttaagta tatggctgga
    21661 aaatccagaa catattgaag aagcattaaa aaggtcagca gcaacaggag caagtgtggc
    21721 tgaaagtaat ggtataggta atttggagaa tgtggaagat tatataaaaa atataagagt
    21781 cagaaaggtg gtataaaaat gggaaagaaa atgattctag gggtaacagg ttgtccaact
    21841 ggaatagctc atacttttat ggcagaagaa gcattaaaaa attcagccag ggaattaggg
    21901 tgtgacataa aagttgaaac gaatggagct ataggtgtag aaaataagtt aacagcaaaa
    21961 gatatcgaaa tggcagatgc aataatagtt gcttgtgata aaaatgttga tatggacaga
    22021 ttcaatggaa agccggtaat agaagtacct gtcaaagaag gtatacataa agctaatgaa
    22081 cttatacaaa gatgtattga tggaaaagta gctattagaa aaggaaactt atcaagcagt
    22141 aaaattgcag aagaaggaag tttatcgttt ggtcaaaagt tgtataaaga tttaatgaat
    22201 ggtgtatctc acatgttacc tttagtagta gcaggtggag tacttactgc tatatcattt
    22261 ttatggggga tttattcatt tgacccaaat tcagaacaat ataatcaaat agcagctaca
    22321 ttaaaatctg ttggtggata ttctatgaac ttgatggttc ctgtattggc agcatttata
    22381 gctcaatcta tatcgggaag agctggtatg ttggcaggac ttgttggagg aatgatagca
    22441 tttgatacag gttcggggtt tttaggtgga atcatatcag gatttttagc aggttactta
    22501 gttaaactat ttgtacattt gttaagaaaa ttaccacgac aattagaagg tcttaaatcg
    22561 atttttataa ttcctattgt cagcgtaggt cttgtgggtg tagcaatgtt gttacttggt
    22621 gggccatgtt cagcacttaa caatgctatg atgaactttc tatcggggtt acaaaattca
    22681 agccctataa ttttgggaat agtaatagga tgtatgtcag catttgatat gggaggtcca
    22741 gtaaataaag cagcatatgt aactggaact atgcttctag gacagggaaa ttatttattt
    22801 atggcaggtg tatctgcagc ttgtataaca ccaccattgg taatagcaat agcatctact
    22861 ttatttaaaa atagatttac agaagaagat agagcagcag gattaatcaa ttatatacta
    22921 ggtagtactc atataacaga gggggcaata ccttttgcag caaaaaatcc attaaaagtg
    22981 ttgccagtac ttatgattgc atcatcaata tcatcaattt taacttatat aatgaaaata
    23041 gaagtaccag ctccacatgg agggtttttg gttctaggat tagtcaataa gccactttta
    23101 tgggtagggt gtatatttgt aggttcatta gtaggtgctg tactttatat gatggttaca
    23161 cctaaagtag taaatgagaa aaatactata gaaaatacaa atgcaacaaa agatgaacat
    23221 aaaaatgaaa gtgcagaatc ggaaaacgta tctaataata tctctttata tagcgaagaa
    23281 acagtaattt tagatgttaa gggaaaaaat aaaacagatg taattgatga aatgattgga
    23341 atacttgata aatctggtgt tcttttagat aaaaagaagt ttaaagaaga aatccttaaa
    23401 agagaagaaa tatcatctac aggttttggt atggggatag cgataccaca tgcaaaaaca
    23461 gatgcagtaa aggtaccacg tgtagcagta ggtgtatcaa aaaaagggtt tgactttgaa
    23521 tcagaagatg gaagcttggc acatattata tttatgatag cagctacaga taatggtgat
    23581 aatttacact taaaaacatt atctcaacta tcttcaaaat taatgaatga agaattttta
    23641 aatgagttat taaatagcaa aacaagtaga gaaatagtat caaaattaaa taacgaagaa
    23701 attaaatcag tataaacata taggaggaga aattatggca ttagtgacaa ctaaacaaat
    23761 attattagat gcacaagaag ggcattatgc agtaggtgca tttaatgttg aaaatatgga
    23821 gatggttatg gcagtaatag aggctgctga agaacttaaa tcaccagtaa tattgcaaac
    23881 aacaccttca acaataaagt atgcaggact agattattat ttagcgaatg taaaggtagc
    23941 atcagagaag gcaagtgttc cagtagcaat gcacttagac catggttcaa gctttggact
    24001 agctatgcag gcacttagag ttggatatac ttctattatg attgatggtt ctcatgaaag
    24061 ttatgaagat aatatttcaa tatctaaatc tgttgtagat gcatgtatgc cttcagatat
    24121 acctgttgaa gctgaattag gtaaagttgg aggtaaggaa gacgatttag atggtggtga
    24181 tgggggagca tatacagacc cattagaagc aaaagagttt gtagagagaa caggggtatc
    24241 atctttagca gtagcaatag gtacggcaca tggattatat aagggagagc caaagcttga
    24301 tttagataga ttatctgaaa ttagggatgt tgtatcagtt ccattagtat tacatggtgg
    24361 gtctggaata ccagatgaag ttattaagga atctataaaa agagggattt gcaaggttaa
    24421 ttatgcaact gaattaagga ttgcttatag caatggggtt aaggatgtgc ttaattctga
    24481 cccagaggta attgacccta agaaatatgg taaaaaaggt ttagagtcag ttaaagattt
    24541 tgttaagagt aggatggaag tttgtggatg tgtaggaaaa gcaatagaga tagtgaagtg
    24601 ttaataataa agggagagaa taagtaagat taaattaagg ggtgtctcaa aatgaacttt
    24661 ttagttcagg aggcactctt ttttgtatga aattaaatat attttcatca ttccgaaaat
    24721 aaaaaagaag attatatcta ttttgagaca gcatcttatt ttattttttg attgagattg
    24781 taattgagaa atttgaaata aggaagatga tataattaaa taaaaaattt ctttctaagt
    24841 ttaaattaat attaaaaagt tataatatgg taagagttat ccaggtagat gacttctatg
    24901 ctggatatat gataaaataa ttttgtatgg gatgtataat aatcataaaa taagagtatt
    24961 gcattagttt tttaactaaa acatgtaaat aggaaaaata atttaataac tgtgtagtaa
    25021 aattttgaat ttaatataag acactaatta ttaaggcgta cgactaaaat atatatttaa
    25081 attattttat gaacactgga gggaaagtca tatatgatta caggagaatt aaggagcaaa
    25141 gtagacaaga tttgggagac attttggact ggtggattaa ctaatccatt agaagttata
    25201 gaacaattta catatctttt atttattaaa ggattggatg aagtagaaac aaaaaatgaa
    25261 gcagaagcaa ctcttttggg agtttctttt gaaaggatat ttgatgatga acatcaacat
    25321 ttaagatgga gtaagttttc taatgaagga aattcagaaa aaatgtatga aatagtacaa
    25381 aatgaagtgt ttcctttcat aaaaaaatta catggaaata aagactcagc ttatgcaaag
    25441 tacatgggag atgcaatatt taaaattcct acacctctta tgttatctaa aatagttgat
    25501 ggtattagta atttagaatt aagtaaggat agagatacaa agggagattt gtatgagtat
    25561 ttattatcca aagtggctac agcaggtact aatggtcaat ttaggactcc aagacacata
    25621 atagatatga tagtaagact tattaaacca acaccagaag atattatagt agacccggca
    25681 gcaggttcag caggtttttt agtatcatca caacaatact taagggataa tcattcaagt
    25741 cttttcttag tgcaagggct gaaggagcat tttaataatg gtatgttcta tggatttgat
    25801 atggatagaa ctatgcttag aataggtgct atgaatatga tgttgcatgg cgttgacaat
    25861 cctaatatag agtataagga ttctttatcg gaggttaata cagataaaga taagtttaca
    25921 ttggtgttag caaatcctcc ttttaaggga agtttagatt atgaagcagt gtcagcagat
    25981 ttattaaaag tgactaagac aaagaaaaca gagttattat tcttggcact ttttttaaga
    26041 gttttaaaaa ttggtggaag atgcgcatgt atagtgccag atggagtatt gtttggttca
    26101 acaggtggac ataaatcgat aagaaaagaa atagttgata atcataaatt ggaagctata
    26161 atatcaatgc caagtggagt gtttaagcct tatgcaggag tatctacagc tataatgata
    26221 tttactaaaa caggaacagg tggaactgat aaggtatggt tctatgatat gaaagctgat
    26281 ggttattctt tggatgataa gagaaatgag ataaaggata gtgatataga ggatattatt
    26341 aagagatttg ataatcttga tggtgaagta gatagaaaaa gaacagagca aagctttttt
    26401 gttgatgtgg aagagataag agagaatggg tatgatttat ctattaataa gtataaagag
    26461 gtagtgtatg aggaagtagt ttatgatgct cctagtgtga ttataggtag agttagggag
    26521 ttagagaagg aaatagctgt tggattagaa gaattagaaa gaatgttaga ggtatagggt
    26581 atggagtata taaaattaaa tgaattgtgc tatataaata ttggcaaaac accatctaga
    26641 aatacttctg attattgggg aagtggtaat agatggttat caatttctga tttgaaagaa
    26701 aaatatatat caaaatcaaa agaagagata acagatttag ctgttgaaaa agctaatatg
    26761 aaattagtac ctaaaaatac cgttgtaatg agttttaagt tgtctatagg tagagttgcc
    26821 atattaaaag aagacatgtt tacaaatgaa gcaattgcta actttcaaat aaaaaataat
    26881 gaacttataa catctgaata tttatattat gctttgagaa ctttaaattt taataatact
    26941 gatagagcag ttatgggtgc aactttaaat aaaagcaagt taaatgatat aaaaatacca
    27001 tatttcacaa tttgtataca aaataaaatg gtaaaagttt taaataaagc tcaagagtta
    27061 ataaataaaa gaaaagagca aatagaagca ttggatgaat tagttaaatc aagatttatt
    27121 gagatgtttg gaaatgtgat tactaatagt aaagattggg atacagagtt attaggagag
    27181 atatcaaact taaaagctgg aaaaaatata aaagcaaaag atatttatga aaaaaattca
    27241 catgaattat atccttgtta tggaggaaat ggtttaaggg gatatgtaaa aatgtattca
    27301 cataagggaa catttaatct cataggtagg caaggagcat tatgtggtaa tgtaaaatat
    27361 gtaaatggaa agttttatgc tacagaacat gcagttgtag tacaacctaa agttgatata
    27421 aatagttact ggctatattt tacacttaaa gagttagatt taaatcgatt atcaactgga
    27481 gcagcacaac caggtcttac agtaggcaaa ttaaatgaag ttgaaatacc taaagtacct
    27541 gtatatcttc aaaataagtt tgtggacttt gtcagacaaa tagacaaatt gaaatcaaga
    27601 atggaagata gcttaaaaga actagaaaac aactttaact cattaatgca aaaagctttt
    27661 aaaggagagt tattcaacta aaatacaagt attaaaatgg gggaataaaa tctatgaatc
    27721 aaatagagct agatttatat catgacatgg taaatatcta taaagaagct gatttacaat
    27781 gtaactataa gccaacaaga tttttacaac tgataaataa caaaggagcc ttattagctg
    27841 ctaaagaatt aatcaataaa gaaggagcta ctgaaggatt tacaaggcta tgggagtgta
    27901 agagattaga tttatcatta gaagtattag tattaaaaga aaagtataaa gagttgttta
    27961 ctgatgaaga aagagaaata tgtataaata gattaaaaga ttatggatat aaattcaatt
    28021 aagcaatatt acctaataaa caagaggtga ttaaatgaga aatataccaa atgagattaa
    28081 aaactcaata ataaatgtat ttccaaaaga aaatactcta gcaatcataa tatttggtag
    28141 ttatggaaca gataaacata catcagaaag tgatatagac atagcttgga ttcctatcaa
    28201 aaaagtacca attacagaac ttgcaactaa gacaaaagct ctaagaaata tattagacat
    28261 tgatgtagac ctaaaaattg ttacagataa ttatacaata gcacttaaaa aagctatttt
    28321 agaaggtaaa gttatttatg aatctaaaga ttttaaggat tatataaata aattctattt
    28381 tgaaaacagt gatgtaatag atatcttaga ttggagggac atgtatggta attagaaaca
    28441 aattatcttg gaactcaata gaagaagtaa atagggattt tggttcttgc ttgtatgacc
    28501 tgcaaaactt caaaatctta tatgataagg aagaaatgcc tgagttatgg gctagataca
    28561 taaaagaagg ctttaaaagc tatatggtat catttctaga attaacaaaa gcaatgttgt
    28621 attataaatc aattgattta aatataaaat ctaaaaactt ttatgaccac cttttaactt
    28681 gtgagtatca caatttatta cctaaaaatt cttctatagt tatagaaaca cttagaaaat
    28741 tgagaaatga tgattctcat ggatatgata taccacagtt tgaagatatg tacgaattgt
    28801 ttacagaaaa tgaagaagta tttgttagta taagaaacag ctgtaagaaa tagtttttag
    28861 agagatttat tttaactaag ggacaaaagg gggagaaaag ccttgggtaa ttttgatttt
    28921 ttacaaaaga agaaaaattt caatagtttt aatactgctt gcttagaagc agaaaaaagt
    28981 atactagtta gcccatctac ttgtgcaact ataacaagaa gagctttaga gcttgcagta
    29041 aaatggctat atactaatga tagtgattta ttattgcctt atcaagataa tcttagtacg
    29101 ttgattcata ataatagttt tatagaactt atagactatg atatgttacc tttattaaaa
    29161 tatatagtta aattaggtaa tttatctgta catactagtg cgaacataga gagaggagaa
    29221 gcaatacttt cacttaacaa tttgcatcaa tttgttagtt ggatagacta ttgttattca
    29281 gatgaatata cagcagaaga ctttaatgaa gatttattac ttcatgggga agagaaaagg
    29341 acgagaccag acgaacttaa aaatctttat gagagattaa gttcaaaaga taaaaagctt
    29401 gaagaagtaa taaaagaaaa tgaggaatta agaaagtcac taactgaaaa gagaaaagtt
    29461 aatactgcaa actatgattt caagatagac gaagtaagcg aatttgagac aagaaaaaga
    29521 tatataaata tagaattaaa gcttgcaggt tgggaatttg gaaaagatat atgggaagaa
    29581 atagaagtac aaggtatgcc taatgaaagt ggtgtaggat atgtagatta tgttctttat
    29641 ggtgaaaatg gtaagccatt agcagtagtt gaggcgaaaa gaacgagtaa agacccgaaa
    29701 attggtcaac aacaagctaa attatatgca gattgtttgg aaaagagata tagtcaaaga
    29761 ccggtaatat ttttgactaa tggttttgat atgtatattt gggatgacta ttcagatagg
    29821 aaagtttatg gattttataa aaaatcagaa ttacaactta tgatagatag aagaaagagt
    29881 aagaagtctt taacttctgt aacaataaat gatgaaatat ccaatagata ttatcaaaaa
    29941 gaagctatta gggctgtatg tgaggctctt gaaaataagc aacgcaaaac tcttttggta
    30001 tgtgctactg gtacaggaaa aacaagaatt gctatatcta tagttgatgt actttcaaga
    30061 cataattgga ttaaaaatat attatttcta gcagatagaa aagcattggt aaagcaagct
    30121 aaaaaaagtt ttactaagct attacctaac ttggcgttat gtaatttatt ggatagcaaa
    30181 gatagcccag aagatgctag gatgatattt tctacatatc caacaatgat gaatgcaatt
    30241 gatgatacga agtctaagga tggaaagaga ttgtttacac ctggtcattt tgatttaatt
    30301 ataatagatg aatcgcatag aagtatttat aaaaagtata agtcaatctt tgattatttt
    30361 gattcttact taatgggtct tactgctaca ccaaaggatg agatagataa aaatacttat
    30421 tcagtttttg acatggaaaa tggagtacct acatatgctt atgagtataa taaggcagtc
    30481 gaggatggat atttagtaga ttatactagt attgagttta agactaagat tatggaagat
    30541 ggaattaagt atgatgagct ttctgatgaa gagaaggaag aatatgaaaa tacatttaat
    30601 gatgatgaga gtattggaga cgaaataggg aataatgcag taaatgagtg gctatttaat
    30661 tctgacacaa tagatttagt tttaaataag ctaatgacag aggggttgaa aatagaagga
    30721 gaagaaaaga ttggtaagac tataattttt gcaaagaata caaagcatgc tagggcaata
    30781 gttgaaagat ttaataaact gtatccaaag tatggtggga actttgtaaa agcagttgac
    30841 tatagtataa attatgtaga ttctataata gatgattttt ctgataagaa taaattacct
    30901 caaatagcag tttcagtaga tatgcttgat acaggaattg acatacctga aatactaaat
    30961 cttgtttttt tcaagaaagt gagaagtaag actaagtttt ggcaaatgat aggtagagga
    31021 actagattat gtccagattt attaggtgtt gatatggata aggaaagatt cttaatattt
    31081 gatttttgta ataattttga attttttaaa tttaatccta aaggatttga gggaaataaa
    31141 acagaaacac ttactgaaaa attatttaat ataaaggtaa gtatggttaa agaacttcaa
    31201 gatattaaat atatagaagg tgagtatgca gaacttagaa aagaattgtt agaagaactt
    31261 ataacttctg taaaagcttt aaatgaggat agttatatag ttaggatgaa tttaagttat
    31321 gtacataagt ataaaaatga aaatgtttgg agcaatattg gagcagtagc acaaaatgag
    31381 ataagagaat atatatcacc actcataact tcatatagtg atgatgaatt ggctaaaaga
    31441 tttgatgtag ttatgtataa tatacaactt gcatacttac aaaataataa tgcaagtaaa
    31501 ggtataaggc atgtaatggc tacagcagaa aaactttcaa aacttgggac tataccacag
    31561 attcagcaac aaaaatatac tattgaaaaa gcaatgacag aggatttttg ggaagattca
    31621 gatatatttg aggtagaaga agtacgtata tcattaagag agcttattaa gtatcttgaa
    31681 aagtctagtc aaaagatata ttatacaagt tttgaagata tgattgtagc agaagataga
    31741 aatgattctg tatataatgc aaacaatctt aagaattata aaaagaaggt agagtattat
    31801 ttaaatagcc ataaagatga attagcaata tttaaattaa gaaataataa aaagataaca
    31861 aagcaagatg ttgaaacgtt ggaggaaata ctcttaaaac agttaggtaa ttgtgatgat
    31921 tataagaagg agtttggaga tactccagta agtcaattag tgagaaaact agtgggattg
    31981 gatagagaag ctgcaaatga agctttttca gagtttttaa ataataagag ctttaatact
    32041 aagcaaatac attttgttaa acttatagtc gattatgtag tcaagaatgg atttatagag
    32101 gataacaaag tgttgatgga agacccattt agaactgttg gaagtataat tgatttattt
    32161 gaaaatcata ttgaagagag aaataaattg attaaaacta taaataaaat taaagaaaat
    32221 gcatcagaga taggctaatg atgttattta gtgaaaaatt attgttaatg atgtagagaa
    32281 taaatttatt agtaggtagt ctaatattgg gttgaaatag ataactagat atggataatg
    32341 taaatataca aagagaggta ttgaataatt atgcaatatc tctttttaat tgataaataa
    32401 ctatgaatta ctcatgaaga agattaaata ataattttta aaattaagta tttgtatatt
    32461 ttatagaata atcatgtatt tactttaata aaataaaaat aggtataaaa aggtatataa
    32521 taaggctttc atatacattt atgagtcaaa attttgatat aatatataga tacatggagt
    32581 tatgaatgga atttatttaa cttatagggg gagttaatat gaaagcagga gaaattgaat
    32641 ttcttagtta tttagagggg tcaaacaaaa gttttgtagt gcctgtatat caaagaaatt
    32701 ataactggaa gaaagaacaa tgtaaaagat tgtttgatga tttagaggat ataatagatt
    32761 ctggatttag aactcatttc ttagggtcag ttgtatctat atatagtatg ggaaaagaat
    32821 atttaataat agatggacaa caaagagtga ctactgtgtc aatattattg attgccatgt
    32881 gtcaaataat aaaagagttt ggggaatcaa aaggaattat tacaacagaa gaacagatta
    32941 aggagtgtta tcttataaac aagcactcaa gagataaaga tggaaaaata aagttaaaac
    33001 caatagcaga agatagaaaa gcgtactatg aactatttga agaagaaatg aatacagata
    33061 gaaattctaa tatagtttca aattatttat atttttataa tagaataaaa gaagataata
    33121 tatcaataga tgatttgttt gatgctatac agaaattaac tatagtagaa atagagttaa
    33181 aaaatggtga agatgaccct caattaatct ttgaaagtct aaattcaact ggcttagatt
    33241 taagtgaagc tgatagagta agaaactttg tgttaatggg tagaagctct aagttacaag
    33301 aggaatttta taataattat tggtataaaa ttgaaaaaaa tacaagtagc aacgtaagta
    33361 gttttataag agactatttg actataaagg agagaagtat accgaataaa aataaagtat
    33421 attttagttt taaaaggtat gtatcagata atggtataga tatagaaatt ttattaaaag
    33481 atttacttaa attttctaac tattatagag agatatctta ttcatgtact gatgataatg
    33541 atgtaaacaa agttttgaaa aggataaaca aacttgaaat atttgtatca tatccgttta
    33601 tattagaatt atttgatgat tataatgaag gtataataaa taaaaaagta cttgtagaat
    33661 cccttaattt agtagaatgt tttatattaa gaagaatagt atgtgatgta cctcctaatg
    33721 cactgaataa agtatttatg aacttaggaa gggaaattaa aaaatttagt gattataaag
    33781 aaaactattc taatatatta agatatattt ttataaataa gaaatcatct caaagatttc
    33841 ctactgacag tgaattttca aatgcttttg aaactaaaga tatatataat actaagtcaa
    33901 agaataagtt atatatactt gagaggttag agaattataa taataaagaa attgttgatt
    33961 tagaaaattt aatcaataat aatatgctaa atatagagca tataatgcca cagacattaa
    34021 caaataaatg gaaggaatca ctaggagata actataagga gatacatgat aaatatttac
    34081 atacaattgg aaatttaact ttgacaggat ataattcaaa actaagtaat aaaacatttg
    34141 aagaaaagaa agaaatggaa gatggattta aagatagtag actttatcta aataaatata
    34201 tttcatctat agataagtgg aatgaagaag aaataaaaaa tagaactaag atattactag
    34261 atagagctgt agaaatttgg aaatatccaa gtgttacata tgaacctcca agtaacagtg
    34321 aaaatatatt tacgttagag gatgatgatg ttaattttac aaatgaaagt atagtatcat
    34381 ttagtatatt aggagagtca tataaggcta aaaattggac agaattttat gaacaagttg
    34441 ctcttacttt atatgatttg gattctgtaa ggtttaacag tattgtagac aaaacttata
    34501 aaagagattt tatagataat attaagtcta gagatgcata taaattaaga aaaggtatta
    34561 aaatatcaga aaatatatat ttagaaacaa atttaaatac agaggctaaa ttaaatataa
    34621 ttagattttt acttaatgag tttgatatag agttaaatga agtaatattt tccataaaat
    34681 aatgaaatat atgcattttt tgcgtaatag aatattaatt atattaaaaa taaaatagca
    34741 cctcttgata tagatttata gctcaaattt atatcaagag gttacgttta attaaagtaa
    34801 tacaaatact atctaattaa attattaata aaaatgttat ataaaaatga gtatatagca
    34861 ttttaaagag taataaacct tacataataa atgatttatt aatatgaact agaagtattt
    34921 ttactatatt gttttataaa acctttaggc attttactta taaaatatac caccataata
    34981 caagacaaag gtttgtctac tatattacta agaatccttg gaaagaacgc agcagtaaat
    35041 atcttctgac cagactgaac taaaaaacca accaataaat cagcaccact tccacttaaa
    35101 ccaccaaaca ataaaactga aataggagta ccaattaaag gacaaacaat agataaaatt
    35161 actcctgtaa taatagcttc tttataacca aaaccttttt tcttagaaat atatccaaca
    35221 ataagaccaa gaacaatatt tacaagagca aaaggtatat ttgtaggacc tgatataaca
    35281 cctaaaacta agtttgtaca cccaccaata agagcacccc aaaaaggacc taatacgaca
    35341 gccccaagta cagtaccaac tgtatctaaa aataataatg gtatatttat catagatata
    35401 accataccta agacaatatt aataactatg cacattgaag aaaatgtgac ttttttagta
    35461 tccaattata atacctccta aaaaaatata tacatttatc atagtataga ttataaaaga
    35521 ttactaattg tatatttcta tatagaatat tggaaatata gaacaaataa ataatataat
    35581 tatcaaaata tgtagtatca ttaaaattaa gaataactaa ggagattatt atgaagagaa
    35641 atagagagat tgtagtagta tgtcattgta tattaaattg taattctaaa gtagagggat
    35701 tgtcagaatt taagggagca caaaatatag caatagattt aataaataaa gggtatggaa
    35761 taattcaact accatgtcca gaaatgatta tgtatggaat aaataggtgg ggacatgtaa
    35821 aagaacagtt tgacaatttg ttttttagag aacaatgtag gcaaatgtta atacctcatg
    35881 taaggcagtt tgatgaatat ttaaaaaatg attatgatat aaaaagtatc attgcaatag
    35941 atggcagtcc aagttgtggt tataataaga catgtagttc taaacgttgg tttggtgagt
    36001 taagtggttg caatgaattg atggataaaa taaatgatat acaaatggtg gatgggaaag
    36061 gtgtttttat agaagagcta gaaaagctac ttaaagaata taatttaaat atacctatag
    36121 ttggatttga cgagcttaga gaagataagt agagatataa atatttattg tctcattatg
    36181 aaacaccatt atatttggca gaaaaatatg actgatggac agtacctgaa tcatttttaa
    36241 gtggagtaat tagtacttat aaagatgtat ccactagttg aaagaaaatg tagagaggta
    36301 ataaatgaat taagaagtag ataatatata taaaggagtg tctaaaagtg aactttttag
    36361 tttatgagac actctttttt tatattaaat caagtatatt aaatcaaata tattttaatc
    36421 attttagcaa tgaaaaataa atttatctat attttgagac aacatctttt ttagttttat
    36481 ttatttgttg tcgtttttga cttggtatac aattgtatat tttcttaaat aatttaataa
    36541 attatttata attataaaat ccattgtttt gaacaatatc taatatatta ttatctgtat
    36601 ttataaggtc actatacata ttttcaagtc cgatattatc tacataatag gcaaacttta
    36661 ttcctgtatg tgttttaaaa aaactagatg tatataggta tcatcacaga tattaaaagt
    36721 ataaataaaa gatattaaga acgactttca agcaacatat tagaaaattt tactaaaatc
    36781 cggaaagcga ttgacaaaca aagtcaaata aagtatatta ttggtaagtt agtttaatgt
    36841 acaaatgaat tatatataaa acctaattta taaataacga tactaaacga cattatttaa
    36901 cttaagctac acaaagatag aatattatac cattgattta ggaggtagcc gtgataaaaa
    36961 tatctgaaga ctatattgaa aaaagatttg acaatgaact attgcgaata gaagcatggg
    37021 gaaaaaactc tttacgtatt aggtcgtttg tagaccaaaa ttttgttgat gaaaactatg
    37081 ctcttaacga aaaacctaag ttaaataaag atgatattac aattaataaa aatgaagatg
    37141 gaagtgcaat cattaaaaat ggaaagataa aagctgtctt agaccataga gatagaatta
    37201 ctttttataa tgataaaaat gaaatattat tgaaagaata tattcgatta agagctgtaa
    37261 aacatgatga tggtggagaa gatgtaggga caattgagat taccaaagac tttaactcta
    37321 ctctaaagtt aaaatcaaga gaatatcgac caagctatag tggagaattt gaagtaacta
    37381 ctagatttga atctgagcct tctgaaaaga tttttggaat gggacaatat caacataaat
    37441 ttttagattt aaagaatact gttttggagt tagcccaaag aaattctcaa atatcagttc
    37501 ctttttatat ttctagtcta ggttatggac ttttatggaa taatccggga attggaaaag
    37561 tatcttttgc taaaaatatg acagaatgga agatgttttc tacaaatttt atagattatt
    37621 ggattacttg tggagaaagc ccaaaagagt taaataaaaa ttattctcaa gttactggaa
    37681 ctgttcctat gatgcctgaa aaccttttag gactatggca aagtaaacta agatatagaa
    37741 cttcagaaga agttttagat gttgtgaagg aatattctaa aagaggaatt aagctttcat
    37801 caattgcaat tgactatttt cattggccta agcaaggtga atataaattt gacttagatt
    37861 attggaaaaa tcctaaagaa ttagttaaaa aattgaaaga agagtattca gtagaaccaa
    37921 tagtatctgt atggcctaca gttcaaagtg atgcagaaaa ctacaatgat tatttagaaa
    37981 atggctattt agtcaatgtt aatcgtggtg ttagaatgac aatgcaaatt caaggaaata
    38041 cagtctttgt agatatgaca aatgaaaatg caagagaata tgtttgggat agaatagata
    38101 aaaattataa acaacttggg attgactatt attggttaga tgtagctgaa cctggctatt
    38161 ctgtgtatga ttttgataac tatagatata aaaaaggaaa tgtcttaagt tgtggtaata
    38221 tttatcctat tgattattta aaaatgattt atgatggttt acataatgac atagagtctg
    38281 ttgtaactct tgtaagagga gcttgggcag gagctcaaaa gtatggagct ttagtatggt
    38341 ctggtgacat tgattctagt tttgaagctt ttaataatca agtaaataca gggttaaata
    38401 tgggactggc aggtatacca tggtggacta ctgatattgg aggtttccat ggaggaaatc
    38461 ctaaagaccc agaatttaga gaacttatgg taagatggtt ccaatatgct actttttcac
    38521 caattttgag aatgcatgga gatagattgc ctcatagtaa accattatca aataaaggtg
    38581 gaggctcaat ggtaactgga gctcctaatg aaatatggtc ttatggtgaa gaagttgaag
    38641 taattcttac aaaattcata aaaattagag agagcttaaa aacttattta aaaaaactga
    38701 tgaaagaagc gcatgaagat ggaacaccag ttatgagaac tttattttat gaatttcctg
    38761 aagatgataa aacttgggag gtggataata catatatgct tggagatgaa attttagtag
    38821 ctccaataat gaattataaa gatagaagta gaaaagtata tttaccaaaa ggacatacat
    38881 gggaaaatat atttagtgga gttagttatg aaggtgggaa aacttatgaa gttgaatgcc
    38941 cactggaaga aattcctatt ttcctaaagc aagattccag ttataatttt aaagaactaa
    39001 aaaatatttt gggagaggtg aaatcataat ggagccacaa ttatggcaat tattattaat
    39061 cgtattatat ggatttttta taaactatga aaaaaattca acaatgtttg gaacatatca
    39121 accagtaaca gcaggtttta ttactggtct tatcttagga gatataaata cagggttata
    39181 tattggaggt acattacagc tattatcatt aggtattagt aactttggtg gagcatcaat
    39241 acctgattat caaacagcaa gtatagtagc cacatttata actattacga ctaagcaaga
    39301 agcatctgta ggaatctcta taggtattcc agttgcttta ttgatggtac aattagatgt
    39361 tcttagaaat actattggta tatggctagt tcataaagca gaagatggag ctaaaaaagg
    39421 aaattataaa aatataacct atatgcaaat gttaggagta ctattaacag cagctacaac
    39481 aggtattcca gttgctctat ctgtaatatt tggaccttct ttaattaata ccattttgaa
    39541 atatacacct gaatggttaa caggtggttt aacagtagct ggaggactac ttccagcagt
    39601 agggatagga ttattactta gatatcttcc tgctaaggag tattttagtt acttagtaat
    39661 tggttttgtt ttagcagtat atatgaaagt accattatta ggtgtagctt taattggtgg
    39721 agcaattgca cttataattt acaaaaagaa tttggaaaat caagaacaac aatatactgt
    39781 agtaggaggt atggacgaag atgagtgata aggtatcatt aaataaatca aatgaaaagg
    39841 tgaaaaaaag tgtcttatgg cgctggtttt ttactagttc tgtttcttca aattatgaga
    39901 agatgcaggc cttggcatac tgttatgcag tactaccttt tttaaaagta gtatacaaag
    39961 ataatccaga aaagttacaa agtgctgtat taaatcattt acaatttttt aacactaatc
    40021 cttgggttgc cccttatatt ttgggtatta atgtagcaat ggaggagtta tcaagtgaag
    40081 agactgaaga ggctgttgct tcaattaaaa caggcttgat gggaccagta gcaggtttag
    40141 gagatagttt atttgtcgtt attccgtgga caatttttgg agctatagca gctaatatgg
    40201 cgatagatgg aagtccattt ggaatcttac tgtggattgt agtgagtgta gcattgaaat
    40261 tattaagtat accactattt aatgctggtt atacatcagg tactaagcta atatcttcaa
    40321 ttgaaaaaag tttaaagatt ttaactgaga gtacatctat tttaggttta atggttgtag
    40381 gtgcattaat accttcagtt gtaaaagcta atgttgcttt gaactttcaa caaggtgatt
    40441 ttacaatgaa aggtcaagaa atattagacc aaattatgcc tgggttagtt cctgcaattc
    40501 tagttgtgat tgtttattgg gctcttaaaa agaatataaa acctatatac ttaattttag
    40561 gagtaatggt tgtttcaata attttatatg cattaggtat tttaaaataa ggaaaggatg
    40621 atattagata tggaaatgaa aggtattaaa aatattcgta tagatgaccg tttaatccat
    40681 ggtcaagttg caactatgtg gagtaataaa ttgggtgtta caagattaat ggtagtaaat
    40741 gatgcagtag ctaacaattc tgttcaaaaa caagttttaa gaatggctac acctgcaggt
    40801 atagcgtcat ctataattac agaagagact gctataaaaa atattactgc aggaaagtat
    40861 gaaggacaaa atgtattatt aattgttaag tctcctgtgg accttattcc atttgtagag
    40921 gcaggattta aaatacataa tataaatgta ggaaatatgt caagtagaaa agatacaaca
    40981 gttctaagac ctaatatcag tgttactgaa gaagagaaag aagcatttaa aagactttta
    41041 gatgaaggaa ttgaaattac aactataatg acgcctgatg ataagaaaac ttatcttgta
    41101 gacatcttat agatttaaag aggtgtatta aattgaatag tattattttg atgagtcatg
    41161 gcaatatggc tgaagaagtc ttaaattctg caaaaatgat aataggggat tctatagatt
    41221 atcctactgt aaatatgaaa caagatgatg gaatagaagg aacaatagaa aaattaaaaa
    41281 aagctttaga agtgtacaag gaaagttcag aaataattat aatggcagat ttactaggtg
    41341 gaactccatg caatgctgct ttattagtag ccaataatga cagtcgaatt aaaattattt
    41401 cagggctaaa tttaggtatg gttattgaat cagctttttt agatacaaag gaattggcag
    41461 caaaattaga gaaaataggt aaagataata tttctatggt tgaacaaaat gcaagtttat
    41521 tatctgatga tgagtaggaa gattatatta atatttaaaa cgaaatactt atgttaatat
    41581 ttaattgata tacaattttt aaaaaatata gctaatcttg cataaactat ataataaatc
    41641 taattttaga atgtataatt taaagggtta tggctgtata tagtcataac ccttatattt
    41701 cattaattaa agttaaattt tgtatattca aaaagctctt gatatttacg gcagagccac
    41761 catatagaga tgcctcagac cctaatgatg aaatttctat atttattcct ttgttaaaag
    41821 agctaactaa catatctttt gtaatctgta atatctcagg gatatcacta attatttggc
    41881 tatttaaata tattatttca ggagcataag ttgtaatagc attatttata gctattgtta
    41941 agtaactaca aaactcatga ataacttttt tggcattctg gttatcttca taatatagtt
    42001 gttttactat atcagaatct attttaggaa tattttctaa agaagaaagc tgttcaaata
    42061 cctttttttc tgaacaatac tgttctaaac agccacgatt tccacaagga catagtttac
    42121 cattaggcat gatgatagtg tgaccaattt ctccactcat accatttctg ccactatata
    42181 atttattatt tattataatg cctgaaccaa atccactgtg aatactgaga ctaagtagtg
    42241 agttatgtac agttgaaaaa gtattctcag ctaaagctgt taagtttgct tcattttcta
    42301 tgtgaatagg aaagtcatac tttttgctaa ggatgctata taagtcaatc tcattaagat
    42361 tgtaatatgg agtaaacaat actttatttt cacaagtaat cccatgtata gccaaagtaa
    42421 gaccaattac cttataagga gtgtctattt tggatatgtt ataactattt ataatttcat
    42481 ctatcaattg tataacattg tctttactta cttgtatatc tgttaatttc ttagaattta
    42541 taatagttcc atctaagtaa gatagagaag aaaatatata gtcgtatcca atgtccatac
    42601 ttaaagaaat ccctgcacat ttattaaata ctaataatat gggttttctt ccgccactat
    42661 gagtactatt tccaattcct atttcatgaa ctagagattc atcaataagt tttttggtaa
    42721 tagcggatat agttgcttta ttcaatccta tagtagaagc tatacttgcc ctagaaatag
    42781 gaccattttt tataatttgt tcaagtacca atctttcatt catttctcga atagtgtatt
    42841 tatcagtaac caatttgata ttcctcctta aaataatatt gtaatacttt ttacacaaaa
    42901 ataaaaggtt attttgcatt gacaaagata attaaatatt ttattattag ttcataagtt
    42961 agtttaatat actaacaaaa ataaagcaag taaaatatac ctaaaatata aaaaaattag
    43021 gataggaaaa cgatagttat gaagtggcat tcaaggaggg atgaagttgg agtatgtatt
    43081 aggtgtggat attggtacat caggtacgaa aacagtttta tttgataaac taggaaatac
    43141 tataaaaagt tgtacatatg aatatcctct tatacaagag aaatcaggtt gggcagagca
    43201 ggatgcaaat gattggtgga aagctgtagt cgaatctata agagaagtag ttcaaagttc
    43261 aaatataagc agtgaatgta taaaaggaat tggattatca ggacagatgc atggtctagt
    43321 tatgctggac aatgaaggta aaacattgag aaactctata atatggtgtg accaacgaac
    43381 agtaaaagag tgtgaggaga taactgattt agtaggtgaa gaaagactga ttgaaataac
    43441 tgctaatcca gcacttacag gatttacagc atcaaagata ttatgggtta gaaacaatga
    43501 accagatata tataaaaata ctaataaaat acttcttcca aaagattata taagatacaa
    43561 attgacaggt gaatatgcta cagaggtatc agatgcaagt ggtatgcagt tgctcgatat
    43621 aagaaaaaga gattggagcg atgaagtact agaaaagtta aatatagata aaaatctttt
    43681 aggtaaagtt tatgaatcac aagagattac aggttatgtg actagagatg tagcaagttt
    43741 gacagggctt aaagaaggaa caatagtggt aggaggagct ggagaccaag cagcaggagc
    43801 tataggaaat ggaatagtta aagatggagt agtttcatct actataggta catcaggtgt
    43861 agtatttgct tatacaaaag agccaaaaat tgataaagaa ggtagagttc atactttctg
    43921 tcatgcaata ccaaatacat ggcatgttat gggagttact caaggagctg gcttgtcatt
    43981 aaaatggttt aaagataatt tctgtcaaag tgaagtggaa gtttcaaatt ctttaggtga
    44041 agatgtatat gagattataa ataatcaagt aagtaaagta ccaacaggat gtaatggatt
    44101 gctttactta ccatatatga tgggagaaag aactcctcat cttgaccctt atgcaagagg
    44161 tgtgttcttt ggattatctc caatccattc taaaaaagaa attgcaaggt caataatgga
    44221 aggtgtttca tatagtctta aagactgtat ggatataata gagaatctta atatagacgt
    44281 taatgaagta agagcctcag gtggaggtgg aaaaagtaaa gtatggagac agatgcaagc
    44341 agatatgttt aatcaagatg tatatactat aaattcaagt gaaggaccag cacttggagt
    44401 tgcaattctt gcattagtag gagctggaat atatgaaaat atacaaaaag catgtgatgc
    44461 tataataaaa acttcaacta aattagaacc aataagtgaa aatgtagata catacaatat
    44521 atatcataga ttgtataaaa gaatatacaa atctttgaaa gatgacttta agttattaga
    44581 tgaagtagtt aatgcaaaaa taaatgatta aattagctta aaaagaaaaa taaagataga
    44641 aaatagtttt aaattaagaa aacatactac atatattata tgtgtaaaat attaggagga
    44701 catgaaatga gtgaaatatt taaaggaata ggacagataa aatttgaagg agtaaaatct
    44761 gacaatgaat tagcatttag atactacaat cctgaacaag tggtaggtaa taagactatg
    44821 aaagaacact taagatttgc aatgagctat tggcatactt tatgtggaga gggtaatgac
    44881 ccatttggag taggcacagt agagagacct tggaataata taacagatcc aattgagata
    44941 gcaaagatta aagttgatgc aggatttgaa tttatgtcta agatgggaat tgagtatttc
    45001 tgttttcatg acagagatat agcaccagaa ggaagagatt tagaagagac aaataagata
    45061 ttagatgaaa tagttgagta tataaaagtt aatatggaaa agacaggtat aaagttatta
    45121 tggggaactg ccaatatgtt tggaaatcca agatttgttc atggtgcatc tacaacatgt
    45181 aatgcagatg tatatgcata tgcagcagct caggttaaaa aagctatgga aataactaag
    45241 tacttaggag gagaaaattt tgtattctgg ggtggaagag aaggttatga aactttactt
    45301 aacactaata cagaacttga aatggataac tttgcaagat tcttacaaat ggctgtagat
    45361 tatgctaagg aaataggatt cactggacaa ttcttaatag agcctaagcc aaaagagcct
    45421 actaaacatc aatacgattt tgatactgct actgttttag gatttttaag aaagtataat
    45481 ctggataaat acttcaaaat gaatatagaa gcaaaccatg caacacttgc aggacatact
    45541 ttccaacatg aattaaatat agcaagaata aacaatgttt taggtagtat agatgcaaat
    45601 caaggagacc tattattagg ttgggataca gaccaatttc caacaaatat atatgatgca
    45661 acacttgcta tgtatgaagt gttaaagcaa ggtggaatag caccaggagg atttaacttt
    45721 gactctaaag taagaagagc atcttttgag gtagaagact tattcctagc ttatattgca
    45781 ggaatggata catttgcaaa gggattatta atagctcata aattattaga agatgaggta
    45841 tttgaaaact ttactaaaga aagatatgca agttttagtg aaggtatagg taaggatata
    45901 gtagaaggta aggttggatt taaagagtta gaatcttatg cactgcaaat gccagttata
    45961 aagaataagt ctggaagaca agaaatgcta gaagctatac ttaacagata tatatatgaa
    46021 gtagatacaa tttctaataa gtaattgtgt agtaattaaa tttggatgtt tgaataaaat
    46081 aacaggatat gttcttgttt ttattctaaa acggacaaag ataagtattt gtaaaaattc
    46141 tcataatcat gataaattaa ggatatagac ttaagtataa ttagtttata tccttaaaat
    46201 ttctataaaa aataatcatt tctatagtta tcaatatttt taattatcaa tatctttctc
    46261 aactaataaa taaaaatcag taaagtatct aaatttaagt cattataatg gataaatttt
    46321 ttagcatatc ttttttagta tatcactgat aagaattt
//
LOCUS       DAEORZ010000014        45574 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEORZ010000014 DAEORZ010000000
VERSION     DAEORZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45574
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.014"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            153..2387
                     /locus_tag="KRH39_000699"
     CDS             153..2387
                     /locus_tag="KRH39_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8510623.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGEGTIEIINSKGKPLFIGKNTELIKDLSHKYKDSADQP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITIGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPGSTYMMVAINIKDFKLINDCFGSEKGNMTLKHLYNVLYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRISVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKLICNKYNVPTYMIDLELTESIFLENPEAVD
                     VIDDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIYY"
     gene            2640..4670
                     /locus_tag="KRH39_000700"
     CDS             2640..4670
                     /locus_tag="KRH39_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8510624.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKAFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKDWKSIDFIITTSK
                     IVIDLPKTVIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEEMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            4667..5119
                     /locus_tag="KRH39_000701"
     CDS             4667..5119
                     /locus_tag="KRH39_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8510625.1"
                     /translation="MSKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLED
                     VLKREETLSTYIGHGIGLPHSQSIGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            5121..6176
                     /locus_tag="KRH39_000702"
     CDS             5121..6176
                     /locus_tag="KRH39_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBG8510626.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDDSIELIFEEL"
     gene            6243..6551
                     /locus_tag="KRH39_000703"
     CDS             6243..6551
                     /locus_tag="KRH39_000703"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBG8510627.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSDIIKKSDALIKKIKDTFQ
                     "
     gene            6655..7920
                     /locus_tag="KRH39_000704"
     CDS             6655..7920
                     /locus_tag="KRH39_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBG8510628.1"
                     /translation="MKKLPIKTVVQRLLDLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKIIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDKRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVDKTSNIRHVLLVNAIECGRWRK
                     WVIGEQKNLTTEEIFKNEVLSNEILDIAGHYTFNNDDVKKEIEVLYANLSKNNIDGQR
                     FVVDHIKRPLRDYEECYNLKGVTTRILNK"
     gene            8008..8724
                     /locus_tag="KRH39_000705"
     CDS             8008..8724
                     /locus_tag="KRH39_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG8510629.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKGISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVEWVLNFSKERNINLGIITNGPSTHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEDLFYKPDFV
                     ILEYKDLISLFIKICSYKNM"
     gene            complement(8919..9212)
                     /locus_tag="KRH39_000706"
     CDS             complement(8919..9212)
                     /locus_tag="KRH39_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBG8510630.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(9218..9433)
                     /locus_tag="KRH39_000707"
     CDS             complement(9218..9433)
                     /locus_tag="KRH39_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510631.1"
                     /translation="MRILSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            complement(9977..10489)
                     /locus_tag="KRH39_000708"
     CDS             complement(9977..10489)
                     /locus_tag="KRH39_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG8510632.1"
                     /translation="MLEVIKNRRSIRTYKDKNIEEEKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHVEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPVKLVPYALISIGYSEEKNEFIDRFDKN
                     KIHRNVYKNR"
     gene            10939..11415
                     /gene="purE"
                     /locus_tag="KRH39_000709"
     CDS             10939..11415
                     /gene="purE"
                     /locus_tag="KRH39_000709"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBG8510633.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            11421..12119
                     /locus_tag="KRH39_000710"
     CDS             11421..12119
                     /locus_tag="KRH39_000710"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG8510634.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            12131..13498
                     /gene="purF"
                     /locus_tag="KRH39_000711"
     CDS             12131..13498
                     /gene="purF"
                     /locus_tag="KRH39_000711"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBG8510635.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMRKYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            13492..14568
                     /locus_tag="KRH39_000712"
     CDS             13492..14568
                     /locus_tag="KRH39_000712"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBG8510636.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            14562..15155
                     /locus_tag="KRH39_000713"
     CDS             14562..15155
                     /locus_tag="KRH39_000713"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBG8510637.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            15148..16680
                     /gene="purH"
                     /locus_tag="KRH39_000714"
     CDS             15148..16680
                     /gene="purH"
                     /locus_tag="KRH39_000714"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBG8510638.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKAKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            16691..17941
                     /gene="purD"
                     /locus_tag="KRH39_000715"
     CDS             16691..17941
                     /gene="purD"
                     /locus_tag="KRH39_000715"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBG8510639.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDKFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKEIEINYSDEEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMLDGNLVTNGGRVIGITAKSVTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            17966..21772
                     /locus_tag="KRH39_000716"
     CDS             17966..21772
                     /locus_tag="KRH39_000716"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBG8510640.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDEDGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKEQKIFES
                     GVKYFR"
     gene            21978..22850
                     /gene="rfbD"
                     /locus_tag="KRH39_000717"
     CDS             21978..22850
                     /gene="rfbD"
                     /locus_tag="KRH39_000717"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBG8510641.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENTEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            22959..23828
                     /gene="rfbA"
                     /locus_tag="KRH39_000718"
     CDS             22959..23828
                     /gene="rfbA"
                     /locus_tag="KRH39_000718"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBG8510642.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            23877..24434
                     /gene="rfbC"
                     /locus_tag="KRH39_000719"
     CDS             23877..24434
                     /gene="rfbC"
                     /locus_tag="KRH39_000719"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBG8510643.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            24472..25455
                     /gene="rfbB"
                     /locus_tag="KRH39_000720"
     CDS             24472..25455
                     /gene="rfbB"
                     /locus_tag="KRH39_000720"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBG8510644.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            25485..26153
                     /locus_tag="KRH39_000721"
     CDS             25485..26153
                     /locus_tag="KRH39_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG8510645.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            26162..26590
                     /locus_tag="KRH39_000722"
     CDS             26162..26590
                     /locus_tag="KRH39_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBG8510646.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDIIQGLLIDMYR"
     gene            26614..26928
                     /locus_tag="KRH39_000723"
     CDS             26614..26928
                     /locus_tag="KRH39_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG8510647.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            27080..27355
                     /gene="flgM"
                     /locus_tag="KRH39_000724"
     CDS             27080..27355
                     /gene="flgM"
                     /locus_tag="KRH39_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBG8510648.1"
                     /translation="MDIKNVSSNILVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            27360..27776
                     /locus_tag="KRH39_000725"
     CDS             27360..27776
                     /locus_tag="KRH39_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBG8510649.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            27794..29104
                     /gene="flgK"
                     /locus_tag="KRH39_000726"
     CDS             27794..29104
                     /gene="flgK"
                     /locus_tag="KRH39_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBG8510650.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            29123..30055
                     /gene="flgL"
                     /locus_tag="KRH39_000727"
     CDS             29123..30055
                     /gene="flgL"
                     /locus_tag="KRH39_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBG8510651.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            30071..30463
                     /locus_tag="KRH39_000728"
     CDS             30071..30463
                     /locus_tag="KRH39_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBG8510652.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            30457..30669
                     /gene="csrA"
                     /locus_tag="KRH39_000729"
     CDS             30457..30669
                     /gene="csrA"
                     /locus_tag="KRH39_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBG8510653.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            30691..31086
                     /gene="fliS"
                     /locus_tag="KRH39_000730"
     CDS             30691..31086
                     /gene="fliS"
                     /locus_tag="KRH39_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG8510654.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIAKTAILD
                     KNTQKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            31086..31442
                     /gene="fliS"
                     /locus_tag="KRH39_000731"
     CDS             31086..31442
                     /gene="fliS"
                     /locus_tag="KRH39_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG8510655.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            31463..32986
                     /gene="fliD"
                     /locus_tag="KRH39_000732"
     CDS             31463..32986
                     /gene="fliD"
                     /locus_tag="KRH39_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBG8510656.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            32989..33324
                     /gene="fliT"
                     /locus_tag="KRH39_000733"
     CDS             32989..33324
                     /gene="fliT"
                     /locus_tag="KRH39_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBG8510657.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            33426..34298
                     /locus_tag="KRH39_000734"
     CDS             33426..34298
                     /locus_tag="KRH39_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBG8510658.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            34391..36532
                     /locus_tag="KRH39_000735"
     CDS             34391..36532
                     /locus_tag="KRH39_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG8510659.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQVQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            36556..38433
                     /locus_tag="KRH39_000736"
     CDS             36556..38433
                     /locus_tag="KRH39_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG8510660.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            38426..40540
                     /locus_tag="KRH39_000737"
     CDS             38426..40540
                     /locus_tag="KRH39_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG8510661.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTSLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTEDLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            40549..41544
                     /locus_tag="KRH39_000738"
     CDS             40549..41544
                     /locus_tag="KRH39_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBG8510662.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNITFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAVCNRTYYKNINK"
     gene            41636..42868
                     /locus_tag="KRH39_000739"
     CDS             41636..42868
                     /locus_tag="KRH39_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBG8510663.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            42888..43832
                     /locus_tag="KRH39_000740"
     CDS             42888..43832
                     /locus_tag="KRH39_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBG8510664.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            43926..44333
                     /locus_tag="KRH39_000741"
     CDS             43926..44333
                     /locus_tag="KRH39_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HBG8510665.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            44420..45511
                     /locus_tag="KRH39_000742"
     CDS             44420..45511
                     /locus_tag="KRH39_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBG8510666.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
BASE COUNT        18445 a         4581 c         8210 g        14338 t
ORIGIN      
        1 ccattatcta tggaatgtta atgaaatgtt aagatggcat tcttgaatat taagaaatag
       61 aatactataa tatagtgtga aggattctta gtaaaaaaat ttataagtgt taatatacat
      121 taaaaaatag gtttataatt tggaggaaaa aaatgaaatt atcaaggtat aagaaaaata
      181 gtttagttct atctataatt ttatgttcac ttattatttc aatccttgta attactgttt
      241 ttaataccgt aaaactacac aatatatcta ataaacaatc taaaaactat ttaaatgatg
      301 tatctacaca gattgtaatg aatgttgatt ctaaaatcaa atttatttta tccgacttaa
      361 gaataatggc agattttatt aagcaatatg aaggtgatag tcgatatgag tatttatcta
      421 aaagaaagac tagttataag tattatgatg ttggaataat agatttagaa ggtaaagctg
      481 agtttttaag tggaaaaaag ttcgacttaa agaatactgc ttcctataaa aaaggtattg
      541 aaggtaaaga atactcagaa gtaatagagt caatgggttt tgttctttat agtgtaccta
      601 tatttaatga taatcatgaa gtatctagta ttttaatagg tgtatccaat aaagaaagta
      661 tgaatgagat tcttaatata gataacttta atggagaagg aactattgaa attattaata
      721 gtaagggaaa acctcttttc ataggaaaaa atactgaatt aataaaagac cttagccata
      781 aatataagga ttctgcagat caaccttggg cacaaaagat gcttaatgat tttaaaaatg
      841 ataaatcagg gaatattaca ataacctcat caaaaggagt acaatgctta ttaacttatc
      901 atccaattac aataggttta aatgattggc actttcttct catagtacct gaagatgctg
      961 tacttggtga acttaataag ttaaattctt ttacaataac aatgacattt ataattacat
     1021 gtattattgg aattataaca ttaattttat atattataag aagaaaatat gtaaatcaaa
     1081 tagaaaatat tgcatattta gatagtataa caaatggtat aaattcaact aagtttagta
     1141 tgctagtaaa acctttaatt tcagaatcac ctggtagtac ttatatgatg gtagcgataa
     1201 atataaaaga ttttaagcta ataaatgatt gttttggtag tgaaaaggga aatatgacac
     1261 taaaacatct ttacaatgtt ttgtataaaa atattgatga aaatgaagaa tttgtgtgcc
     1321 gtcatgatgc agaccttttt tatttgctta ttaagaatag accagtgtta gaagccctgg
     1381 agttattata taaaatagaa gctgatacaa attattttaa taaaaataga gaaaatccat
     1441 actttttaag aatatcagta ggtatttaca caattgaaaa tcataatgaa gatttaatta
     1501 caatacaaga ccatgcaaat acagcaagaa aaagctttaa taaaactcat caaagtgatt
     1561 tttcttttta tagtgatata gaaagaaaac gactaatatc tgaaaaggaa atttcaaatt
     1621 taatggaaaa agctcttgaa aataaagaat tttttatgtg cttacaacct aaaattgata
     1681 taaaaagtgg gaaaattagt ggagcagagt ctcttgtgcg ttggaaaaat tcagaaaagg
     1741 gcataattta tccaagtgat tttattcctc tttttgaaaa aagtggattt atatgtaagt
     1801 tagatttata tatactagaa gaaacatgca aattgatttc taagtggata aaagaaggta
     1861 aagaagtgtt gagaatttct gttaatgtat cacgccaaca tttgaatgat aaattgtttt
     1921 tagaaaaata taagcttata tgcaataaat acaatgtacc aacatatatg atagatttag
     1981 aacttaccga atctattttc ttagaaaatc cagaagctgt agatgtaata gatgatattc
     2041 attctaatgg atttaaatgt tctatagacg actttggatt tggatactca tcattaggaa
     2101 tattaaagga ttttaaagtt gatataatta agttagaccg ttcattcttt gtaagcaaaa
     2161 ataatataga ccgtggtaag gtagttatta agtctattat tgagcttagc aaaaggctag
     2221 gtatgaaagt aactgctgaa ggcattgaag aattagaaca agttgagttc ctaaaaaatg
     2281 tgggatgcga ttatattcaa ggttatgttt tttctaaacc attattaatt tctgactttg
     2341 aaaattttgc atatgaaaat aataaaatta aaaatatcta ttattaaata tataaataaa
     2401 aaaggcaagt tatataatta aaaatataac ttgtcttttt tatttatata tttaataaaa
     2461 atgtatttgc agattttgta aaaataattt tcaaatgttg taagttgtag ctaaagcttt
     2521 attatattat tctgaatata gaaagatagt tactgtctta gataaaaaaa ctaaaacata
     2581 cataataata taaaatttgc tatattaata actctaatta ttttggaagg agggaaaata
     2641 tgataagtgg gagaatgtta ctcatcatga attttctaaa aggaaaaaat aagacaagtt
     2701 acaaggatat tagcaaacaa cttagtatag aagaaagaaa agttagatat gatataaata
     2761 atttaaatat tgttttgcgt tcattaaaca agtcagtaat acaaaaaatg aacaaaggtg
     2821 tgttaattat tcctgatgac ttccaagtag tatgtataaa taataacgaa tatgtatttt
     2881 ccttaactga gcgagtatct attatgaaaa ttatatcaat gtttaaaata gataatctaa
     2941 acttagaaaa gttgagtaaa gaatttcagg tttctagaag ttcaattaaa aatgacctta
     3001 atgttttgtc aagtgaatta gaaagaaacc aaataactat tacttatgat aaagctttta
     3061 aaataagtgg agaagaagac attatatata agcaaagatt gaacatatta aaatcatatt
     3121 cttatctttt tggtaaggaa aaatctgatt taagtgtatt tgagaagtat ttagtgaacg
     3181 tgattgaaaa aatattcaaa gggatttatt tgattgacat atataattgg tctatgaatt
     3241 tattattgca aatgggatgg actttaaatg atgaatcttt tacatggtat gtgtcaaaca
     3301 ttttcctttt tacatggtat atacattcag atgaaaaaca tcctttagaa tctgcttcat
     3361 taagtgagcc gtgttttgaa aataagtttg accaagaatt tgaaacaatt attcagaaaa
     3421 agttgacgaa ggaacaaatt tttctgttaa agagttttgt attttttaca aataaatatg
     3481 caagtttaaa cgaagatatg gatttgatta gtacagaaat tatagttcaa gatttaattt
     3541 acaatatgtc agaaatatta gagattagct ttaaagaaga tatgattctt tataaaggat
     3601 tactcaatca tattgcccca ttgatagaga gaatcaaaca gaatatacag atttatgaag
     3661 aattacttca tgtaatacca gataaatatc aatatgtact tgaagctact aaggtttcta
     3721 ttcaaaaaaa cagagtttta agtcaaataa agaatgaaaa tgaaacaata cttttggcaa
     3781 tacatttctt aggaagtata catagaaatg agaggaataa ctacaaaaat atattgttag
     3841 tttgtggatt tggatatggt gtaattgcta tgttaaagga tagtttggta aatgattacc
     3901 agataaatat agtagagagt attccatcat ataagttaca agattataaa gactggaaaa
     3961 gtattgactt cattataaca acatcaaaga tagtaataga cttgcctaaa acagttattg
     4021 agataaatcc atttttacaa gaagaggatt atttgaagtt tgaagaaaaa gggataaaaa
     4081 gaaagaacgt cttaactaat tatatgtcaa taaaaaaaag attggatttt ttagaagaag
     4141 atgcccaaaa aagagtgttg agtattatat gtcaagaact tggatacagt gatgagagaa
     4201 tattatttag acaacttaat ttaagtgatt taatagggat tgataccata aggataattg
     4261 atgaagagat gaaatgggaa gatgccgtaa aaattagttc aaatatactt gctaattgtg
     4321 attttgtatc agcacaatat gctgaaaata ttatagatat tttaaaaaat gtgggttttt
     4381 atgctgtaaa agataacgaa tttgctttac tacatggcaa tgatagttca ttagtcaaag
     4441 taagttcgat aagtttatta atatgtaaag aagcagttag ttttggagat aaaaaggtta
     4501 aaatcatatt ttgtctagca agcagagata aaaaagagca aattcctgca ataatcaatc
     4561 taactaaaat ggtttataag actaatttta ttgctatgtt agaaagtgca aagacaaaag
     4621 aagaagcaga agttttaatt cataaatatg agaaggaggt cactgtatga gtaaattagt
     4681 taatgtaaaa tgttcattta aaatacatgt taattcaaaa gaagaagcca ttatatcgtt
     4741 agttgatgta gtgtcaaaag aaggatattt aatagataaa aatcaatttt tagaagatgt
     4801 tttaaagaga gaagaaacat tgtcgactta tattggacat ggtataggtc ttcctcatag
     4861 tcaaagtata ggtgttaaaa actcttgtat tacaatagga aagcttgata ctcctattga
     4921 atggacagaa gaaggagaaa aagttgattt aatattttta atatcagtaa caaaagacaa
     4981 tgaaaataat cttcatttaa agattttatc aaaattagca cgtttattga tgcatgaaag
     5041 ttttagaaat caaattagat gttcagatga acaaactgtt tataatctta taaaagaaaa
     5101 aatagaggag gaagattaaa atgaggattt taaagttttt gcaaaaacat cttatgacag
     5161 caacatctta tatgattcca tttgtggttg caggaggaat attatttgct ttatcagtaa
     5221 ctctaagtgg acaagctgct gttcctgaaa caggatggtt ggctaaactg aatcaaattg
     5281 gagctgctgg attggcttta ttcattccta tattaggtgg atatatagca tttagtatgg
     5341 cagataaacc aggcttagct ccaggtatga taggtgcata tttagcaaaa gaagtaaatg
     5401 caggttttat aggtggtatc atagcaggat ttatagcagg atttgttgtt ttacaattaa
     5461 agaaaattaa attagctcct acaatgagaa ctttaggttc tatatttatt tatcctttga
     5521 ttggtacatt gattacaggt ggtataattg tatttttaat tggagaacct atagcttctt
     5581 ttatgacatg gatgacaaac tggttaaatg gaatgtctgg agtttctaaa attcctttag
     5641 gtggaatttt aggaggaatg atagcctcag atatgggtgg tcctataaat aaggttgctg
     5701 ctacttttgc ccaaactcaa gttgacacac ttccatattt aatgggtgga gttggagttg
     5761 caatttgtat acctccaatt ggtttaggat tagccacatt attgtttcca aaaaaattta
     5821 gcaaggaaga aagagattca ggtaaagctt cgctattaat gggatgtgtt ggaattacag
     5881 aaggagctat tccatttgca acatcagacc cagttagagt aataccttgt attatggctg
     5941 ggtcgattat aggaaatatt atggcttttt tattaggatg tttaaatcat gcaccttggg
     6001 gaggtctgat agtattacct gtagttgaca ataggttggg gtatattgca tcagtattaa
     6061 ctggagcagt agttgttgca gtattaatga aattagtaaa aaaagacgtg aaagaagatg
     6121 aagaaatcga agaggattta gatgactcaa ttgaattaat atttgaagaa ttataaatat
     6181 aaatggaagg aatttgtata aaaaagtaaa tataaaacta taaatttaaa ggagataaga
     6241 atatgaaaat tgtagcagta acatcttgtc ctagtggagt agcacatact tacatgtcgg
     6301 ctgaatcact tgaactatca gccaaaaaat ttggtataga aataaaggtt gagactcaag
     6361 gaagctctgg aatagataat gaattgtcac ttaaagatat tgatgaagca acctgtgtaa
     6421 tactaactaa tgatgttgag attagaaata tggaacgttt taagggaaag aaggtagtaa
     6481 gaatgagtgt atctgacata attaagaaat ctgatgcatt aattaagaaa ataaaagata
     6541 cttttcaata aaaattgatt tattttacat ataactcaaa gatgttttta aagaaatttt
     6601 tattaaaagc atctttgaaa atatcttaaa aatagaataa aaggagatat agtcatgaaa
     6661 aagttaccaa taaagactgt cgtacaaaga ttactagact tacaagaaga ggggaaaagt
     6721 gcaactcttt taggaattgg tcctatgtca ccaaacttgt tacaagcaag ttttgaacta
     6781 gcaaaagatg atgattttcc tttaatgttt atcgcaagta gaaatcaagt agatgcagat
     6841 gaactaggtg gtggttatgt aaatggttgg aatcaggaaa cttttacaaa agatattaag
     6901 aagattgctg acaaagttgg atttgatggt ttatattatt tatgtagaga ccatggtgga
     6961 ccatggcaaa gagataaaga gagaaatgac catttgcctg ttgatgaagc aatggagtta
     7021 ggaaaaaaat cttatttagc ggacattgaa gcaggttttg acctattgat gattgaccct
     7081 acaaaagacc catttgaaat tggaaaaata attccactag atgtagtact agaaagaact
     7141 gtagaattaa ttgagtattg tgaaaacgag agaaaaagac ttaatctacc agacataggt
     7201 tatgaggttg gtacagagga aaccaatgga ggtctgactt caactgagac atatgaaaca
     7261 tttataacta gactaaaggt tgaacttgat aagagaggtt tgccaatgcc aacatttata
     7321 gttggtcaaa ctggtacttt aactagaaag acagaacaag taggaacttt taattttaga
     7381 aatgcttatg atttagctca aatggctaag aaatatggag ttggtttaaa agaacataat
     7441 ggtgattact tagatgatgt aactttatta gaacatatac catctcaaat tattgctaca
     7501 aatgtagctc ctcaatatgg tactgaagaa actagggcat atttaaaatt agcagaagtt
     7561 gagtgtaaat tagaaaaaga gggtctagtt gataaaactt ctaatatcag acatgttctt
     7621 cttgttaatg caattgaatg tggacgttgg agaaaatggg taataggaga acaaaaaaat
     7681 ctgactactg aagagatatt taagaatgaa gttttatcta atgaaatatt ggatattgca
     7741 ggacattata catttaataa tgatgatgtt aaaaaagaaa tagaagtact ttatgctaat
     7801 ttaagtaaga ataatattga tggtcaaaga tttgttgtag accatattaa acgtcctttg
     7861 cgtgattatg aagagtgcta taacttaaaa ggtgttacta cgaggatttt aaataaataa
     7921 taggacttat agtggtgagt attgttatac taggtattta tagttataat tatacttacc
     7981 actattaaat ttaggaggac taatattttg attaatttaa tttttgatgt agatgatact
     8041 ttatataatc aattaacgcc tttttacact gcatataata aagtgttttc ttcaataaag
     8101 ggtatttcta ttgaagattt atatatgagt agtagaaagt atagtgatga ggtatttcat
     8161 atgactgaga atggagaaat gccaatcaaa gagatgcata tctatagaat aatgaaagct
     8221 tttgaggagt tgggaaattc tattacagaa aaagatgcac aaagttttca agatgagtat
     8281 atatatcaac aatcacaaat tacactaata ccagaagttg agtgggtttt aaatttttct
     8341 aaagaaagaa atattaatct aggaattata actaatggtc catcaaccca tcaaagaatg
     8401 aagttaaagc agttaaatat tgaaaactgg gttgataaaa gtaatatttt tatctctagt
     8461 gaggttggat tttctaagcc agatactaac atttttagag tagctgagaa tgttatgaat
     8521 ttagatagag aaaatacata ttatgtgggt gattcttata gaaatgatgt attaggtgct
     8581 aaaaaagctg gttggaagag tatatggttg aaccataggg gacatgaggt agaagatttg
     8641 ttttataagc cggattttgt aattttggaa tataaggatt tgatatcttt atttataaaa
     8701 atatgtagtt ataaaaatat gtagtaaaaa aatgttaata atagaattgt ataaaatagt
     8761 taataataac tatataaaat tatatattta gattgtgagc aatctatttt atgttaaaaa
     8821 tagattattt agaagaaggg gaaatcttaa aaaaatttga atatcccctt tttattattt
     8881 aattaaaatg aaatagttag aaatatattt tatttagatt aatcaataac attttgagat
     8941 tctttataac tacatgttaa ctgttgaata ggtttatcat atggtttaac ccatccttta
     9001 ttaatactta attctgtaag tgctgtgtga ccttctacca tttgagttag tgaagctgaa
     9061 tatatagctc ttagttcagg tgtactactt gttaatgcag aattaagata catattggca
     9121 gatgctttag cagatgaaag catacttaac attattactt tatcgtctat ataagtattg
     9181 ttcttaatta gattttctat catatttctc atgttatcta tacctcctca gaaattagta
     9241 tttcattttc aactataaat tgttgtatag ctttaattct tccttctgtt gctaatatac
     9301 tagattctga ttgtcttttt aggtcttcat ctgatattaa catatttatt gctctttgca
     9361 taagtaatcc atcagattcc atttttataa ctgctgctaa agatattttt tcagtttctg
     9421 aaagtattct catttaatca tctcctatat tgtaatttat gctttaatag tatgtctttc
     9481 ttttcaacga aataaccata acactttttt acagtatttt atattctctt tataccaaag
     9541 cttagttagc ttatatacta ataaaatttt gtaggagagt tatcattaaa tacttataag
     9601 ataaattatt taaatatcca ttaaataaaa tttatttgta tgtacttctt caagagtcaa
     9661 tatgagtttt ttcaaaaaat taactttgac aataatcatt aagaggtata ttttcttgaa
     9721 gttgtgatta ttattattaa tttactaaaa tactttaatg aaaaaatgga taactttaat
     9781 tgtaggatat tattaatata tttgaaataa ataaaaatat tgaaggatat aatattcaat
     9841 tggtatataa ataataactg atagttaaat tttactttag aggataatta ggtgcttagt
     9901 caaagttaat gcctttagaa tacaaatgga ttctaaaggc atttttacta tgtatttttg
     9961 tttatattac taaatattat ctatttttat atacatttct atgtatttta tttttatcaa
    10021 atcggtctat aaattcattt ttttcttcac tatatccaat agatataagt gcataaggaa
    10081 ctaattttac tggtaagtct aaagtttgtc taactttatt tactacttta tttttaggaa
    10141 atactcctgc ccaacaagca gctagacctt gattagtaac ttctaataat aaattttgtg
    10201 tgcaagcacc taaatcttgt atccacttac caggttttaa gaatttatct ctatttccaa
    10261 gcactactat acataatggt gcaaactcta catgctttgc tctatgctgt gactttgaga
    10321 gctctttcag tagttcctta tcatctacta taataaattc ccaaggctga gcgttttttg
    10381 atgaaggtgc ttgcatagct gactttagta tttcagttat tttctcttct tctatatttt
    10441 tatctttata tgttcttatt gaacgtctat tttttataac ttctaacata cagattctcc
    10501 tttaaataaa attatttcta tttatataat atcattactt taaattttag ctattactta
    10561 tataaagaat aaactttgta ttggttttat ctaagtaaag ctaacattca gaactaaaaa
    10621 acaagattat aaatccaagg tattaattaa aaaagaagta ataaaaaata atatacaaga
    10681 ttttaaaaag ggtattgagc ttaaaattta attagaaaat caattttgag atagtctgtt
    10741 ttttatttta acaaaaaaac ataaataaag ttcgatacgt agcgaatgaa aaaaatacaa
    10801 gaaatatttt ttttaaaaaa tttcaaaaaa gagttgataa tatatgtata ttaatataaa
    10861 atttaaggtg tataattcgt ataataatac gaatgattta gagtgtaaaa taacttataa
    10921 acggagaggg gaagtctaat gaaagtagca gtagttatgg gttcaaaatc agattatcca
    10981 aaattagaag aagggataaa actgcttgaa aaatatggaa tagaagttgt agcaagagca
    11041 ttatcagctc atagaacacc agaacaactt tcaatattcc taaaagaaat agaagatgat
    11101 actgatgtaa taatagcagc agcaggaaaa gcagcacact taccaggtgt aatagcttca
    11161 caaacattaa ttcctgtaat aggattacct attaaatcat caactatgga tggactagac
    11221 tcacttttat caatagttca aatgccaaaa ggaatcccag ttgcaacagt tacaatagac
    11281 ttaggtttaa acgcagcttt attggcatta caaataatga ctttaaaata cccaaaatta
    11341 aaagaggatt tgaaatcata tagagaagag atggcacaaa aggtgttaga agacgacaag
    11401 aatttaaggg ggtagcagaa atgttattat atgaaggaaa agcaaaacaa gtgtattcta
    11461 cagataatga aaatgaatat gttgtttatt ataaagatga tgcaacagca tttaatggag
    11521 aaaaaaaagc agaaatatct tcaaaaggta tactaaataa taagatatca acaataatct
    11581 ttgaaatgtt aaaagaaaat aatataaata ctcactttat aaaaagttta tcagatagag
    11641 aaatgttagt taaaaaagta gagattttac cattagaagt aatagtaaga aatattgctg
    11701 ctggttctat ctgtaaaaga gttggtcttg aagaaggtgt agtttttgat gagccaattt
    11761 ttgaaataag ctacaaaaat gatgcttatg gtgacccaat gctaaatgat gattatgcag
    11821 tggcaatgaa gttagcaact agagaagaat taaaattctt aagagaagaa actttaaaaa
    11881 taaacgaatt actaaaagca tttttcttaa aattaaacct aaagttagtt gactttaaaa
    11941 tagaatttgg taaggattct gaaggaaata taatattggc agatgaagta tcaccagaca
    12001 cttgtagact ttgggatgta aatacaaatg aaaagttaga taaggacaga tttagaaaag
    12061 accttggaga ccttgtagaa ggttatacgg aagtactttc aagaatgaat aataaatagg
    12121 aggcaaatgt atgtgtggag ttttagggat ttactctaat aaagatgtaa ctaaagaact
    12181 atattattct ttatattcta tgcaacacag ggggcaagaa agctgtggtt tagctctttt
    12241 agatgatgga gaaattaagt acaaaaaaga tatgggatta gttggagatg tttttaagga
    12301 aaatgaatta tctaagttaa aaggaaatat agggattgga catgttagat attcaacagc
    12361 tggaggaagt catgtttcta actgtcaacc tttagttgga agttgtagaa aaagacaatt
    12421 agccatagct cataatggaa atctagttaa tgcaaattat ttaaaagata tgctagaaga
    12481 agatggatat atgttccaaa ctaattcaga tacagaagtg attttatata tacttgcgag
    12541 atattacaaa ggggatatag tagaaagttt aaaagtgaca atggattata taaaaggtgc
    12601 atatgctctt gtaataatga gtcaagaaga attggttgca gttagagacc cacatggatt
    12661 cagacctctt gtacttggta aaaagggtga tgaatatata tttgcatcag aaaattgtgc
    12721 aatagatata cttggcggag aagttataag agatgtagag ccaggagaga taatagtagt
    12781 taaagatgga gaactaaaat catattttta ttcagaaaat tataaaccag ttaagaaaag
    12841 ttgtatattt gaacacatat actttgcaag aaatgatgca actatagaca atgtaaatgc
    12901 atatgaattt agaattaaat gtggtgagag attagctcag aatgaaactg taaaggcaga
    12961 tatggtagtt ccagttccag attcaggttg gccaggagct attggttatg caaatgctag
    13021 tggattaaaa ataagtgaag gcttagttaa aaatagatat gtaggaagaa catttataaa
    13081 acctacacaa gaagaaagag aaatagcagt aaaaataaaa ttaaatcctc tttcaacaat
    13141 aataaaagga aagtctataa ttttagtaga cgattcaatt gtaagaggaa ctacatctaa
    13201 acaattagta aaatctttaa gagaagcagg agcaaaagaa attcatctta gaataacttc
    13261 tcctccagta gcttactctt gttactatgg aatagatact ccaaatcgct ctaaattaat
    13321 tgcatcaagt aacaacgtag aagaaatgag aaaatatatt ggatgtgata gtttaaaatt
    13381 cctagatata gaaggaatgt tagatgcaac agaacataag tctactttct gtaaagcttg
    13441 ttttgatgga gaatacccag ttaagaaaat tgataaggag gaactacttt catgttaaca
    13501 tataaagaat caggtgtaga tatagatgaa ggaaatagag cagtagacct tataaaaggt
    13561 aaaataaaag gaacttatga tggaaatgta gttggagatt taggtaactt cagtggatta
    13621 tacagtttaa aagattttgt aggtatgaaa gagccagttt tgcttgcatc tacagatggt
    13681 gttgggacta agttaaaaat agctcagatg atggacaagc atgatactgt tggaatagat
    13741 ttagttgcaa tgtgtgtgaa tgatttaatt tgtcaaggag caaaaccatt attcttcctt
    13801 gactatatag ctcttgggaa attagttcct gagcatatcg aaaaaatagt tggtggaatt
    13861 gcagatggtt gtaaaatgtc tggttgtgca ctaattggtg gagaaactgc tgaaatgcct
    13921 ggaatgtacg gagaagatga ctatgattta gcaggatttt ctgtaggaat agcagacaaa
    13981 gaaaagattg tttctggaaa caacgttaaa tcaggagatg tattggttgg tatatcttca
    14041 agtggagtac acagcaatgg attttctttt ataagaaaga tatttttaga aacttacaat
    14101 tataaaatgg aacaatatgt tgaagaacta ggaatgacag ttggagaagc acttttaaca
    14161 ccaactaaaa tatatgttaa attagcttta gatgtattag ctaaacatga cataaaagct
    14221 atagctcaca taactggtgg agggcttata gaaaatataa caagagttat acctaaaggg
    14281 ttaggactag atatcaataa aaaatcttgg gagaagcctc ctatatttaa aatgatagaa
    14341 ggttttaatg ctgttgatga gagagaactt cataagagtt ttaacatggg tattgggcta
    14401 gttttaatag tagataaaga aaatgctgat gatgttgtaa actttataaa taatagagaa
    14461 aatgacaatg ctgcttatgt agataaaaaa tatagtgaat tattagaaga taaagcgtat
    14521 ataattggtg aagtagtaga tagtcatgaa ggtgtagaat tatgctaaat ataggagttt
    14581 taatatctgg tggaggaaca aatttacaag cagttataga tgggactgag tctggagaaa
    14641 taaaaggtca ggttaaagta gttatctcaa gtaagcaagg tgcttatggg ctagagagag
    14701 ctaaaaatca taacataaaa gcaatttgtg aaactgatga agacaagata atagaaattc
    14761 ttaaggaaaa taaaatagat ttagtagttt tggctggtta tttaaaaata atcagcccaa
    14821 agctggttaa tgagtttaga aacaaaatga taaacataca tccatcatta attccttcat
    14881 tttgtggagc tggattctat ggagaaaaag ttcatcaagg tgttatagat tacggagcta
    14941 aagtaactgg tgctacagtt cattttgttg atgaaggtgc tgatactggg ccaatcataa
    15001 tgcaagatgt agttaaggta aatcaagatg atgatgcaaa gacacttgca aaaagagtgt
    15061 tagaagttga acataggatt ttaaaagaaa gtataagtct tttttgtgaa aataaattaa
    15121 aacttcaagg taggagagtg tttataaatg agtaagagag ctttaataag tgtaactgac
    15181 aagactggtg tagtggaatt tgctaaagag ttaaataagt tagattatga aattatatct
    15241 acaggaaata catttaaaac attaaaagaa aatggtgtta atgtaatgca agttgaagat
    15301 gttacaaatt ttccagaaat attagatgga agagtaaaaa cattaaatcc atatatacat
    15361 ggtggtatac tttataaaag agataaagaa tctcatgtag aaactgtaaa tgagcataaa
    15421 atacattcaa tagatttagt agctgtaaat ctatatgatt ttgaaggaac tttaaaagct
    15481 ggaaaaagtc atgatgaaat aattgaaaat atagatattg gtggaccatc tatgatacgt
    15541 tctgctgcca aaaactataa agatgtaata gtagtagtag atataaaaga ttatgattct
    15601 ataatagaaa aattaaaaac agatacaatg acattagaag acagaaaaaa attatcatat
    15661 aaagctttct caactacagg aagatatgat gcgttaatat ctagttattt tgctggagaa
    15721 gttggagata cgtatcctga catattaaat ttaactttcc aaaaagaaca aactttaaga
    15781 tatggagaaa atccacatca aaatggtttc ttatattcac agtcaaatgc aaaaaatcca
    15841 atcttaaatt atgagcaatt aggcggtaaa gaactttctt ttaataactt aaatgattta
    15901 catggatgtt tagaagtaat gagagaattt aaagatagtg aagaagtagt ttcagtagct
    15961 ataaaacatg ctaattcatg tggggtaggt ctaggaaaag atgcttttga agcatataca
    16021 aaatgctatg aagcagataa ggtttcaata tttggaggaa tagtaggaat aacttctact
    16081 atagataaag ctacggcaga gaaattaaat gaaatattct tagaaatagt agtagcatat
    16141 gattttgaac cagaagcttt agagatactt aaacaaaaga aaaatctaag aatattaaaa
    16201 ttagctaaaa tagaaaatag tttacaacca tatgaaatga aatatttaga tggaaaatta
    16261 ttgatacaag atagaaataa catattagca gaaaaaagtg aaaatgtaac aaaggcaaaa
    16321 ccaacagatg cacaacttaa ggacatggaa tttggtatga gagtagtaaa aaatatgaaa
    16381 tcaaatgcaa ttgcaatagt aaaaaatgga caaactctag ctttaggttg tggacaaaca
    16441 tcaagaattt gggcattaaa aaatgctctt gaaaataaca aagataaaga ttttacagga
    16501 gctgttttag cgtcagatgc atttttccca tttgatgatt gtgtaacatt agctcatgaa
    16561 tatggaatat cagcagtagt tcaaccaggt ggctctataa aagataaaga ttcaatagaa
    16621 gcttgtgaca agtatgatat ggttatggta tttacaggaa taagacactt caaacactaa
    16681 ggaggatatt atgaagatat tggttgtagg cggtggcgga agagagcatg ctatatgctg
    16741 gaaactaagc aaagaaaaaa atgtagaaaa aatttattgt gcaccaggga atgcaggaat
    16801 tgcaaatgta gcagaatgtg taaatatagg tgatactaat atagaagaac ttttaaaatt
    16861 tgccaaagaa aatgaaatag gtctgacaat agtaggacca gaagtaccac tagttatggg
    16921 aatagtagat aagtttgaaa aagaaggatt aagagtattt ggacctaata aaaaatgtgc
    16981 tcaacttgaa ggaagtaaag ctttttcaaa agaatttatg ataaaacata acatacctac
    17041 tgcaaaatat aaagaatata caaacttaga agaagctata tcagaaatag atagttttgg
    17101 atatccagta gtaataaaag cggacggatt agctgctgga aaaggtgttg taatacctga
    17161 aaatagagaa gatgctatag caactttaaa agaaatgatg tcagataaga agtttggagc
    17221 tgctggagat aaaatagtaa ttgaagagtt tttaaaagga atagaaactt caattctagc
    17281 atttgtagat aatgatacaa tagttcctat ggcaagtgca aaagaccata agaaagtaaa
    17341 caactatgaa caaggtccaa acactggggg aatgggaact ttttcaccta gtgaaatata
    17401 tacagaagaa ttagcaaata aagtaaaaga gacagtatta gaaaaaactt tagaaggctt
    17461 caaaaaagat ggacttaatt ttaaaggtat tttatttgtt ggtcttatga taactgaaga
    17521 tggagaaaaa gtacttgaat ataatgtaag atttggagac cctgagacac aatctgtttt
    17581 atttagatta gaaacagatt tacatgagat aatggaagct atactagata ataagttaaa
    17641 agaaatagaa ataaattata gtgatgaaga agctgtatgt gttatgctca catcaggagg
    17701 gtatccagac agttatgaaa aagggaaaat tataactggt cttgaaaact tggatgatga
    17761 tatagtagta ttccatagtg gcactaaaat gcttgatggt aatttagtta ccaatggagg
    17821 aagagtaatt ggtataactg caaagtcagt tacagttaag gatgcagcag aaaaagttta
    17881 tgaaaacata aagaagataa actttgaagg aatgcactat agaacagaca taggaagata
    17941 atttttctaa aatggaggat aaggtatgtt gaatactgaa aataaagact caatggttag
    18001 aagagtatta gttgaaaaaa gagaaggttt tgaccttgag gctaaagctt taaaaaaaga
    18061 cttggtggaa agtttacaca tagataatat agaaaattta agaatattaa atagatatga
    18121 tgtagaagga atatcagaag aagtttatga aaatgcagca aagacaatat tctctgaacc
    18181 aaatttagat gtagtatatt atgaagaaat accaaaatta aatgatgaaa gagtatttgc
    18241 aatagaattt cttcctggtc aatacgacca aagaggtgat tgggcagctc aatgtgtaca
    18301 aattgtaaat caagggataa gacctgctat taatacagca aaagtttata tattatcagg
    18361 caaaataact gatgaagagt tttctaaaat aaaagattac tgtataaatc cagtagatag
    18421 tagagaagca tctttagaaa aaccagaaac attaaaaatg gaaactgaaa taccaacaac
    18481 agttgaagta ttagatggat ttatagattt agatgaagat ggactaagaa catttgtaag
    18541 tgaaaaagga ttagcaatga ctttaggaga cttgcaacac gttcaaaaat actttaaaga
    18601 tacagaaaag agaaatccaa ctataacaga aataaaagta ttagatactt attggtctga
    18661 tcattgtaga catactactt ttatgactga aatagaaaat gtaaagatag aagatggtaa
    18721 gtttaatgat atagtaaaag aagcgtatca aatgtatctt aactcaagag acaatgtata
    18781 tgtgaacaga cataaagata tatgtttaat ggatatagcc actgttgcag taaaagaatt
    18841 aaagaaaaat gggaaattaa atgacctaga tgaaagtgaa gaaataaatg catgtagtat
    18901 aaacgtagat gttgaagttg atggtaagat ggaaaaatat ctagtaatgt ttaaaaatga
    18961 aactcacaac catccaactg aaatagaacc atttggtgga gcagctactt gtttaggtgg
    19021 agctataaga gacccattat caggaagaag ttatgtttat caagcaatgc gtgttacagg
    19081 aagtgctgac ccaagaacga ctttagaaga tactttacct ggaaaactta tgcaaagaaa
    19141 aataactaca gaagctgctc atggatatag ttcatatggt aaccaaatag gtcttacaac
    19201 tggacaagtt gcagaagtat atgatgaaaa ctttgttgca aagagaatgg aaataggtgc
    19261 agttatagca gcagcaccaa aagagaatgt agtaagagaa agacctgaag caggagatgt
    19321 aatcgttctt cttggaggaa aaacaggaag agatggctgt ggaggagcaa ctggttcatc
    19381 taaagaacat agtgaagagt caatcttaac ttgtagtgca gaagttcaaa aaggagatgc
    19441 tcctaacgaa agaaaaatac aaagattctt tagaaataaa gaagttgctc aaatgataaa
    19501 aagatgtaat gattttggag caggtggagt ttgtgttgct ataggagaga ttgctgatag
    19561 cttagatata aacttagact tagttcctaa aaaatatgat ggacttgatg gaactgagct
    19621 tgcaatttca gagtcacaag agcgtatggc agtagccata aaaaaagaaa ataaagataa
    19681 atttatacaa ttagcagtag aagaaaactt agaagctaca catgtagcta cagtaacaga
    19741 tacaggatat ttaagaatgt tctggaatgg aaaagcaatt gtagatataa atagagagtt
    19801 tttagataca aacggagtaa aacaaactac tgatgttcat gttactaaag tagatgaaga
    19861 aaatacattc tttagctcta atgaaatagt aaaagatgtt aagtgtgcaa gtatgaaaga
    19921 caagtttaca aaagtactat ctgaccttaa tgtttgttct caaaaaggat tagtagagat
    19981 gtttgataat acaattggtg gtaatacagt acttatgcca tttggaggaa agtatcaagc
    20041 gactccaaca caaggtatgg ttgctaagat accagtactt ggaggagaaa ctaatacttc
    20101 tacaataatg acttatggat acaatccaaa ggtaggaaaa tggagcccat tccatggtgc
    20161 cttgtatgca gtagtagaat ctgtgtgtaa attggttgct attggaggaa attatagtac
    20221 aacaagactt acattccaag agtattttga aaaacttggt aacaatccag aaaaatgggg
    20281 taaaccattc tcagcacttt taggagcatt ttatgcacaa agtaaatttg agatacctgc
    20341 tataggtgga aaagacagta tgtctggtac atttaaagat attgaagttc caccaacatt
    20401 agtttcattt gctgttgaca cagtagatgc gaagaaagta gtatctccag aatttaaaaa
    20461 agcagactca aaagtagtaa tgttatgtgt aaacaaggca gaaaatgatg ttgtagattt
    20521 tgaggaatta aagagaaact tagataaagt aagagaactt atacatggaa ataaagtttt
    20581 atcaacatat gcattaggat ttgcaggtgt aggtgaagca ataagtaaaa tggcttttgg
    20641 taataaaata ggatttaagt ttagtgaaga ggctgaaaaa gcatttacag atgataaatt
    20701 atttgaggct agttatggaa atatagtact tgaattagca aatgatgatt taagtatgtt
    20761 agaaggatac aattatgttg tgcttggaag tacagttaaa gaagctagta tatttatcaa
    20821 aggtgaagaa cttgcattag atgaattata taaagcacat tgtagtactt tagagcctat
    20881 attcccaact aaaacagaag aagtaaaatc aaagatagaa actataagtt atatatcaca
    20941 aggagaagct aaaaaatcat cattaagtat agctactcca agagtattta taccagcatt
    21001 ccctggaaca aactgtgaat atgactctgc aagagcattt gaaagagcag gagctaatgc
    21061 aagtataaga gtatttaaga atttaactta taaagacata gaagattcta tagatacaat
    21121 agtaaacgaa ataaaatctt ctcaaataat aatgttacca ggtggattta gtgctggtga
    21181 tgagccagat ggttctggta aatttatagc tactgtattt agaaatccta gagtgcaaga
    21241 agctataaat gaatttttaa ctcaaaaaga tggactaatg ttaggtatct gtaatggatt
    21301 ccaagtatta attaaattag ggttagttcc atatggagaa ataagagttc caagtgaaag
    21361 tgctccaaca cttacttaca ataatattgg tcgccatcaa gctaagattg ctagaactag
    21421 aatttcatct aataaatctc cttggttagc tcaaactaat gttggagata ttcataatat
    21481 agcaatttct catggagaag gtaagtttgt tgccagtgaa gatgttatga gagaattaat
    21541 tgctaatgga caagtagcaa ctcaatatgt tgactttaat aatgaagcta cttatgatat
    21601 tgagtttaat ccaaatggtt cattctatgc tgttgaaggt ataactagtg ctgatggtag
    21661 agtgtttggt aaaatgggac attctgaaag aattggtgag gaagtatata agaatatcat
    21721 aggggaaaaa gaacagaaga tatttgaatc tggagttaag tattttagat aaaactttga
    21781 tgtttaattg atagctagaa tatatatttt agatatttag atttgaagag atgtaataat
    21841 gtgatgataa tcatttaata ttacatctct tttttattta tctaagaaat aataagttca
    21901 acctaagtta aaatacaagc tgtgttataa ttaattatta agtaaaattg aatatttatt
    21961 ggagggcaac ataattaatg aaaatattaa taacagggtc taatggtcaa ttaggaaaag
    22021 aattggttaa ccaattagaa gcaatcaatc aaagtataaa ccaaccaaaa tatgttatat
    22081 tagctacaac aagaagtgat ttggacatat ccaatcagac taatgtagat aattttattt
    22141 tgcataataa acctgatgta gttgtaaact gtgcagcata tacaaaggtt gatgcatgtg
    22201 aagataatat cgaaattgca tacaaaataa atgctttagg agtgagaaat ctagcaatag
    22261 catcagaaaa agttaatgct aaacttattc acatatcaac tgactatgta ttcaatggtt
    22321 tttctaaata tccatatagg gaagataata aaactgaacc caattctgta tatggaaaaa
    22381 gtaaacttat gggagaaaaa tttgttgaac aattctcaca taagtacttt atattgagaa
    22441 cagcatggtt atatggtaat gggaataatt ttgtaaagac catgataaaa ctttctttag
    22501 aaaatacaga ggttaatgtg gttaatgacc agtttggaac tccaacaagt acagtagact
    22561 tagcaaaaat tataataaga atcatggaaa cagaacatta tggagtttat catgctacat
    22621 gtgaaggtga gtgtagttgg tatgattttg ccaaaaagat atttaaacta aaaaatatag
    22681 atataaaagt aaatccaatt aagagtaacg aatttaaatg taaggctcca agaccactat
    22741 attctgtatt ggataatttt atgttgaagt taattggttt aaattctttt agaaagtggg
    22801 aagaatccat tgaagaatat ttagaaagat atgatataaa tgaaaagtaa atgtattatt
    22861 ttgagaaaag aaaataagat attataaaaa atataaatat ttcttttatt tatccgataa
    22921 tcataatatg acaaaaacaa atttaaggat ggaaaaagat gaaaggaatt attttagcag
    22981 gagggtctgg tacaagacta tatcctataa caaaatgtat atcaaaacaa acacttccaa
    23041 tatacgataa accaatgata tattacccta tgtcggttct tatgttggca gggatacaag
    23101 atatattaat aatatctaca ccaagagata taaaagcatt tgaagaatta tttggagatg
    23161 gaagttcact agggcttaac ataacatatg caattcagga gaaagcaaat ggaattgcag
    23221 aagcgtttat aataggagaa gaatttatag gaaatgacag agtgtctctt gtattagggg
    23281 ataatatatt ttatggatat ggatttagtg aaaggttgga gaatgcagtt aaaagaaaag
    23341 gagcaactat atttgggtat catgtaagta atccaaattc gtttggagtt gtagaatttg
    23401 ataaggattt taatgtattg tcaatagaag aaaaacctac agttcctaaa tctaattatg
    23461 ctataccagg attatatttt tatgataatg atgttgtaga gatagcaaag ctagttgaac
    23521 cgtcagatag aggtgaatta gaaataacct ctataaacag tgaatatcta aaacgaggaa
    23581 aactaaaagt ggaacttcta ggaagaggta tggcttggct tgatactggt acacataaag
    23641 gtctattgga tgcttctaat tatgttgaag tggttcaaac aagacaggga ctgtatatag
    23701 catgtttaga agagattgca tatacaaaag gatatataag tagagaacaa ttaatgaaac
    23761 tagccaagcc actgataaaa actgaatatg gtcaatatct attaaaattg gctagtgaag
    23821 atatgtaata tggtagataa aatttattat aaattttatt aagggagttt attataatgg
    23881 gaaaaatcaa tgttataaag actaaaatag aaggattata tatagtcgaa ccaaatgtat
    23941 tttatgatga aagaggatat tttatggaaa gctataataa aaaggatttt atagagcagg
    24001 gtcttgatat gtgttttatt caagataatg agtctagctc aagaaaaggc gttttaagag
    24061 gaatgcattt tcaggttaaa tatcctcaag gaaaattagt cagagttaca gagggagaag
    24121 ttttcgatgt tgcagttgat ttaaggaaag attcacaaac atttgggaaa tgggaaggtg
    24181 taatattgtc tgataagaat aaaaaacaat tttatattcc agaagggttt gcacatggat
    24241 ttttagtttt atcagaaaaa gcagtattta gttacaaatg tacaaattat tattacccag
    24301 aatttgagaa tggctttata tggaatgaca agcaaatatc tattgagtgg cctttaaaag
    24361 gaattgaaga aatattttta tcagaaaagg acaaaaaaca aaaaagtttt aaagatattt
    24421 gtacaagacg ttaataaaaa tttattagaa ttaacattta ggagaaaaaa tatgaaaaaa
    24481 atactgataa ctggaggagc aggatttatt gggagtaatt ttatacatta tatgcttaac
    24541 aagtatgtag attattttat tgttaaccta gacttgttga cttatgctgg taatttagag
    24601 acattaatag aaatacaaga caattccaat tataaatttg ttaaaggaga tatttcaaat
    24661 agggagcagg tgtataagct atttgaagaa gaaaggtttg atatagttgt taactttgcc
    24721 gctgaatctc atgtagatag gtcagttgag aatcctgatt tatttattaa aacaaacatt
    24781 ataggaacac aagtactttt agatgcatct ataaaatatg gagttaaaag gtatcatcaa
    24841 atatctacag acgaagtgta tggtgattta ccaattgata gaaaggattt attctttaca
    24901 gaacaatctt ctatcaatcc aagttctcca tattcagctt ctaaagcatc agcagattta
    24961 ttggtaagtt cttattatag aacatatgga ttgttaacaa ctatatctag gtgttccaac
    25021 aattatgggc catatcattt tcctgaaaaa ttgattcctc ttatgatttt aaatgctttg
    25081 gaaaacaaac aattgccagt atatgggaat ggggaaaatg taagagattg gttacatgta
    25141 tatgaccatt gtactgctat agacttaata atacataaag gaaatatagg ggagatttac
    25201 aatataggag gtcataatga aagaagtaat ctagaagtag taaaaatgat attaaatctt
    25261 ttaggtaaat ctgaagagtt aatatcctat gtaaatgata gacctggtca tgatttaaga
    25321 tatgctattg atgcaactaa aatagagaat gagttaggtt ggaaggcaaa atatgacttt
    25381 gatttgggta taaaagaaac tgttaagtgg tatatagaaa atgaatcttg gtggaaagct
    25441 gttttatcag tataacttta aaatttagaa aggggcgttt tatattgaat gagataagtt
    25501 cactaattaa tatattagca ttgactcaat tatctggtag taatactaca aatcaatgta
    25561 attgtacata taataattca tctggatttg atatgattat gatgaccttg ttaaaagcat
    25621 tatcacaaaa taatcaacat acttctaata gctacaattt attgtctaat tctgagaatt
    25681 tatttaatga acttgataat gtagtaaata ataccactag tagatttata gatgttgata
    25741 caaaagataa aaatgtaaag ttgagaattg aaaatgctgt agatcaagca tcaaaaaaat
    25801 ataacgtaga ttcaaatctt ataaaagcaa taattaaagt tgaatcagat tttaatccaa
    25861 ataccgtttc ttctgctggt gctaagggtc ttatgcaact tatgccagaa aactgcagag
    25921 acttaggagt tacagaccct tttaatatag aacagaatat agatgctgga actagacata
    25981 ttaaggaata cattgatatg tttggaggga gcatagaaat gggattgatg gcatataatg
    26041 gtggtcctgg aagaatgaaa agtagaggag tagaatcaat ctctgacctg tataaaatgc
    26101 caaaagaaac acaaaattat attcctaagg ttatgaaata ttatagaggg taggctttat
    26161 tatgggaata aatatagata agctaaacat aagtccaagt ataaatatgg aggttaaaaa
    26221 actaaaagag agtaataaat tttctgacaa attaaactct ttaaaacaat cttcttcatt
    26281 ggaagatata aaacgaaatt tggaaagtgt aaaaaaggca ggagaaaggc ttgtacttac
    26341 tcaaaactat ggcgatatta gtatatataa aaatgcagta aaagaatacc taaaatctgt
    26401 tgtagataat atgtattcac ttgataaaaa tagtagcttt tgggagcatc agtattataa
    26461 gaatgtaaag attgttgata gtaaacttga ggatatgact agtaagttaa ttagtgaaga
    26521 aaaagaaaac atagatattg tttctactgt tgacatcata caaggcttat tgatagatat
    26581 gtatagataa ggttatacaa ggagaaaaat tttatgaata gcaaaaaaga ggatatatat
    26641 gaattgaatt ttgggcaatt aatagaagaa aaatctggtg ttatagaaga aaatcaaaat
    26701 gtttttaatg caaaagtaaa tgtagctgtt tctataggaa atacaacaga aacaataaaa
    26761 aatatattag atttaaaaga aggctctata ataaaattaa gtaagaatgt ggatgaaaaa
    26821 ctagatatat atgcaaacga tagattgttt gcttatggag aaagcattat tacaaatgag
    26881 aggttatctg taagattatc taaaatagaa gatccagatg taaattagca ttttaaatag
    26941 tatcccttta ggctattctg tatgagttta gctaagggga tatttttata aaaaatttaa
    27001 aatatgtgtg aattatataa atatttttat tttatgagcg ataactaaat tataagtatg
    27061 taattttgga ggtgaaacta tggatattaa aaatgtaagt tcaaatattt tagtgaataa
    27121 ttatttaaag aatacatctg gtataaaaaa agtggaagag gttaaaaaaa ttaataaaac
    27181 agacaagata gagttatcta aatttgcaaa ggaaatggca agtgtgtcaa atatgagtga
    27241 tgagaatatt agaaataaaa aagtcgatga aataaaaaat gcattagaaa atggaactta
    27301 caaaataaaa ccaaaagaaa ttgcgaaaaa aatgatagag gatatgcgag gataaagtca
    27361 tgattacaga cttaaaagtt ataatatatg aagaaaaaaa tataattagt aagatgatgg
    27421 aattattaaa agaacaattt gattatatag ttgaaaaaga cttagaaaat ttaaatatgt
    27481 taaatcctaa actagaagat attagcaggg agcttgcatc catagaaata aagagaagac
    27541 aactttttgg tgatgatatt tcaatgagtg aagttgttga aaatagtaat gatggatact
    27601 taaaagaaat atatacagac ttgaaacaca ttcttgcttt agctaaaaat caacaagaat
    27661 caaatgattc tctaataaag aaagaactta tttttacgaa aaaaatgatt aatttcataa
    27721 aaccagctga taagcaaact acaacttata attcttatgg aaatatcaga aaataaatat
    27781 taggaggaga aatatggcag gattattttc cattttaaat acagctaatt caggaatgaa
    27841 tgcgcaccaa aaagcaatac agacaatttc tcataatata agtaatctag atacagacgg
    27901 ttattctagg caaaggaccg agtttgccac aaatagtcca atgtatatgc ctagtttgag
    27961 taattcaatt gcaagaggac aattagggac tggagtacat gtaacggatg taacaagagc
    28021 tagaaatagt ttttatgatt accaatttag ggcagaagct cataagtatg gtaagacagt
    28081 ttcaaaatat gattattaca atactataga aactatacta aatgaaccat cagactatgg
    28141 aatatctgca ggtatagatg atttttttaa aggatgggag tcattatcaa aggatataaa
    28201 tagtgacagt aaaaaaagtc tagtggtaga aaacgcatct aatttagcaa ctttaataag
    28261 tgaaagttat aagaagcttg ataagttaaa agaagatata gattctaata tagacactga
    28321 gataaaacaa ataaatgaca tgttaaaaag tcttgaagat ttaaataaat ctattgatat
    28381 tatatctggt tctggctcta caccaaatga tttgcttgat gaaagagata gaattttaga
    28441 taacttgagt tttaaactag actttgaaaa tagtgatgtt aaaaatatgc ttgcagatgg
    28501 taaattagaa cttaatgaat taaaaaatcc agatggaact tggaaaacag gtatatcagg
    28561 gaccttacaa ggcttgtttg aaatgcatgg taagattgat acatataaaa gcgatttaaa
    28621 agatgtatct gatggattag ctaaacagat aaatgatgtt tataatacgt ctgcaggaat
    28681 aacagtaaca gattttttta taacttcaaa tgtagatggt gaagatatta taaagataaa
    28741 tccagctata aagagtaacc caaatgaatt aaagcttact gctgatgaag cttcaaaaat
    28801 tgctaagtta aaggatgaaa aaatagatat tggggtggct ggtggaaaag taagtacaat
    28861 aagtgaccac tataaagcct ttgcagaatc aatcggttta gattctcaaa aagtaaatca
    28921 agatgaagca aatcaacgta aaattataaa taatattgat aactcaagaa tgagtgtttc
    28981 aggtgtatct ttggatgaag aaatgacaga gcttataaaa atacaacgtt catatcaagc
    29041 tagtgcaaaa gttatgtcaa cagctgtgca actactggat gttgtaataa atggaataat
    29101 ttaatagaag ggggaattta taatgagagt cagtacaggt atgatgtcta gcagctatct
    29161 aaactcacta caagataatc tacaaagatt ggataaggtt aatagacaag ttaatacaac
    29221 aaaagaaata aacaagctat cagataatcc atataaagca ataaagatat taaactcaaa
    29281 aagtgaaata aagactatgg agacttatgt tgagaattgt aaagatacag ggaattggtt
    29341 agaaactact gatactgcat tagatcagct tggaaaatta ttatcagaca taaagaaaac
    29401 tttggtggca agtggtgatg gtgcatactc agatgatgaa ttaaagactc ttagtaatga
    29461 aactaatgaa aaaatgaaag aaattgcaaa tgctttaaat gcaacacatg aagggaagta
    29521 catattttca ggttcaaata cgggggtacc acctgttgaa tgtatagata atgctgatgg
    29581 ttcagtgagt ttaaagttta attctagtct aaatcttaat aaattaaatg actctttgag
    29641 tatggatata gctcaaggaa taagtgtaga ttataacgtt aaattgagta gccttggatt
    29701 tgacccaaca aaaactgacc catttgaaga gttaaataac atatcaaaaa aattagcaaa
    29761 tccagatgct gatgctataa aagagcttac aacaacttgt ttgggcgata tggaacaact
    29821 aatagaaaat acagtaaatg ttaggtctat atatgggaca aaagcaaata cagtagaggc
    29881 tatgaaggaa aaaaacgatg atagtttgat acagttaaag gatattttgt cacaaaatga
    29941 agaaatagat tatggtgaaa aacttgttca attaaaggca gcagagttaa cttatcaagc
    30001 ttcactacaa acaggaggaa agttatttaa tgtatctata ttagattaca tataataaaa
    30061 taacgcagat atgatgaagg ttacattaaa aaaaggtata ttaggttttg aaaatttaaa
    30121 agaatatgag cttttagata tagaagataa tgattattta aaagaattta attctactga
    30181 agaagaaggt ataggatttg ttgttgtatc tccatttgag attatcaatg aatatgaaat
    30241 agttttaaat caggaaacaa tagaaaaact agaagtgaaa tctccaaagg atgttgtatt
    30301 atttaatata attactcttg gtcagacatt tgaagaatca actgtgaaca tgaaagctcc
    30361 tatagtgata aatataaaaa ctaattttgg aatgcaaata attttacagg atgataaata
    30421 ttcaataagg catccattat tgagaggtga taatggatgc tagtaatttc aagaaaaaaa
    30481 gatgaagcag tacttatagg agaaagcata gaagtaaaaa ttgtaggagt agatggaaac
    30541 aatgtaaaac tagctatatc agcgcctaac aatataagta tattaagaaa agaaatatac
    30601 gaaaaagtaa agagtgagaa tataaaagct acaaataaaa atataaaaat tttaaagtca
    30661 ttaaaataat aagcttggag gaagtaatat atgtatggag aaaatcctta taattcttat
    30721 aaacaaaatg ctgtttttat ggcatcaaag gaacaattgt tacttatgct tgttgatgga
    30781 gcagtaaaat ataccaaaat agccaaaaca gctatcttag ataaaaatac acaaaaagcg
    30841 catagagagc ttataagagt tcaggatata tttacagaac taatggttac attagaccaa
    30901 aatgcaggtc aatgggcaaa ggatatgtac agagtttatg attttgtaag atatgaactt
    30961 tcaagagcta atattagaaa agacattcaa ataatagata atgtgttgcc tgtaatagaa
    31021 caaattaaag atacttggca tgaagctgat aaaaaaagta aagaagaaag aagtaggtac
    31081 aaataatgga attaaattta gctgaattag ataagttatc aaaagaggaa ttactattga
    31141 tgcttgtaga tggaacagta aaatatacca atatatccaa agaagctctt ttaaataacg
    31201 actatttgaa agcacataat gaacttgtaa gagttcaaaa tatatttaca gaactaatga
    31261 ctacattaga ccaagatgca ggtcaatggg caaaggatat gtataaagtt tatgaattta
    31321 taaaatctga attagcaata gctgatgaaa ataaagatat taaaataatt gatgatatat
    31381 tgccaatagt aaaacaaatt agagatacat ggcatgaagt ttacaatcaa ctaaaaatat
    31441 aattggatgg agggataaag gtatgtcaag tataagtcct ataagagtta caggtctttc
    31501 aggaaatttt gatatggaag gcataatcga agctagtatg attagagaca aggaaaaagt
    31561 tgataaagca aaacaagaac aacaaatcgt taaatggaag caagaaatat atagaaatgt
    31621 tatacaagaa tcaaaagatc tttatgataa atatctaagc gtaaattctc ctaatagtat
    31681 agtaagtgaa aaagcatact cttctacaag aataaccagt tctgatgaaa gtattatagt
    31741 agcaaaaggc tcagctggtg cagaaaaaat aaattatcaa tttgcagttt ctcaaatggc
    31801 tgaaccagca aaatttacta ttaaattaaa ttcaagtgaa cctattgttc aacagtttcc
    31861 tccaaatgct agtggagcga gttctttaac tataggagat gtaaatatac caatatctga
    31921 acaagatact acaagtacta ttgtaagtaa gataaactcc ctttgcgcag ataatgatat
    31981 aaaggcttct tatagtgaga tgacaggtga attgattatt tcaagaaaac agactggttc
    32041 gtcatcagac attaatttaa gagtaattgg aaatgacaat ttagctcagc aaattgctaa
    32101 tgataatggt atcacattta taaatgatgc tggtggaaac aaagtggcaa atgtatatgg
    32161 aaagaatcta gaagctgatg taactgatga acatggaaga gtaactcata taagtaaaga
    32221 acaaaattca tttaatatag ataatattga ctataatgta aattcaaaag gaactgcaaa
    32281 gttgacttct gtcactgata ctgaagaagc tgttaaaaat atgcaagcat ttgtggatga
    32341 ttataataaa ctgatggaca aggtctatgg tttagttact actaaaaaac caaaagatta
    32401 tccgcctctt acagatgccc aaaaagaaga tatgacaact gaagaaatag aaaaatggga
    32461 aaagaaagct aaagaaggta tacttagaaa tgatgatgag ttaagaggtt ttgttgaaga
    32521 tattcaatct gcattttttg gagatggaaa aaatattatt gcattaagaa aactaggtat
    32581 caatgaaagt gaaaattaca ataaaaaagg tcaaatatca tttaatgcag atactttttc
    32641 aaaggctctt atagatgata gtgataaggt atacaaaaca ctagcaggtt attcttcgaa
    32701 ttatgatgat aagggaatgt ttgaaaagct aaaagatatt gtatatgaat attctggaag
    32761 ttcaacttct aaacttccta aaaaagcagg tatagaaaaa actgcttctg ctagtgaaaa
    32821 tgtatattca aaacaaattg cagagcaaga aagaaatata agcaggttag ttgaaaaaat
    32881 gaatgataaa gagaaaagac tttatgctaa atattcagcc ttagaatctt tgttgaatca
    32941 gtattcttcc caaatgaatt atttctcaca agcacagggt aattaattat ggataaatta
    33001 aaagaaaaat taaatttata taaagatata tctttacaaa ttataaattt gattgaaaag
    33061 gaagaatata taaagataag tattaagtta ggtgaaagac aagagattat aaatagtgta
    33121 agtgaaattg atagaaatga ttttatacag ctttataata gaatggaatt aattgaaatt
    33181 gatagcagga taagagatat tttacaagga caattattag aagtaaaaaa agaattacat
    33241 gagtataagc ttactaaaca agtgaacaca atgtattata acttaaatag agaaaaagta
    33301 aatattttta ataaaaaagt ataaagttat gtaccatctt gtccgattat ataaatgaag
    33361 aattaataaa aaagattaaa agtagaaatg acaaggatgt caactatact aaggagggta
    33421 aaataatgag agttaataca aatgtaagtg ctttgatagc aaataaccaa atgggaagaa
    33481 acgtaaatgc acaaagtaag tctatggaga agctatcttc tggtgttaga attaagagag
    33541 ctgctgacga tgctgctggg cttgctatat ctgagaaaat gagagctcaa ataaaaggtt
    33601 tagaccaagc aggaagaaac gttcaagatg gtatatctgt tgtacaaaca gcagaaggtg
    33661 cattagaaga aactggtaac atactacaaa gaatgagaac tttatcagta caatcttcaa
    33721 atgaaactaa tactgctgaa gaaagacaaa agatagctga tgagttacta caattaaaag
    33781 atgaagttga aagaatatca agctcaattg agtttaatgg taagaaatta cttgatggaa
    33841 gttctacaga aataagatta caagttggag caaactttgg aacaaatgtt gcaggaacat
    33901 ctaacaacaa caatgaaata aaagttgctc tagtaaatac ttcaagtata atgagtaaag
    33961 caggtataac aagttctaca atagcaagct taaatgcaga tggaacttct ggaacaaatg
    34021 cagctaaaca aatggtatct agcttagatg tagctcttaa agaattaaat acatcaagag
    34081 caaaattagg ggcacaacaa aatagactag aatcaactca aaacaactta aataatacta
    34141 tagaaaatgt tacagcagct gaatcaagaa taagagatac agatgttgct tcagaaatgg
    34201 ttaacttatc taaaatgaat atattagttc aagcatcaca gtcaatgctt gctcaagcta
    34261 atcaacaacc acaaggagtt ttacaattat taggataaaa taattttgta tattggatat
    34321 agaagaaagg gtaaggcttt gctttactcc tttctttttt tacaaatata cttttgaaag
    34381 gaaaatttat atgttactta gtatagttat gattgttaaa aatgaagaaa aaatacttga
    34441 aaaaacttta aaatcattaa ccacattaag aaattctata gaaagtgaac ttataatagt
    34501 cgatacaggt tctacagatg atactataaa aatatctaag aagtacacgg aaaaagtgta
    34561 ttttcataat tggaataatg atttttctag tatgagaaat atatcaatat cctatgctaa
    34621 aggtgaatgg ttacttatat tagatgcaga tgaaatacta attgattctt caactattgt
    34681 aaaatttttt aaagatggtc ttgataaaaa atttaattct gcttcaatac aattaaaaaa
    34741 tctttactca tatgataaaa aattatatgg atattgttca gtgcttagac tttttaaaaa
    34801 cgttgatttt agataccatg gtaaagtaca tgaacaacca atatacaaaa accctatatt
    34861 taataatgtt gctgattttg aacattatgg atatttgttt gaagatgaag aaataagatt
    34921 aaataaggtg aaaagaaatg aaaaactatt atttcaagag ttaaaagaga atgataattc
    34981 tccatataca aactatcaat tagggaaaaa ttttattata ttaggaaaat atcaggaggc
    35041 tttatactat cttgagaaat cttatgaatt atattctaaa ctagaattgg cacctggtta
    35101 cttgataaca agccttgtaa aaacatattt atatttagga aaacataaga agtgtgaaag
    35161 attgtgctta aagtatatta agaaagacat gaataatatt gatatatatt attatattgc
    35221 acaagtacaa gtagatttag gaaagtatga aaatagtata gattcgtata agagatatat
    35281 ttacttgtta gataattatg aaataagtac acaagcaaat agtttatttt cagacactga
    35341 tactgtaggg cttagagatg aggctattat tacactgata aagatttatt ataagcttga
    35401 aaaatatgag tttgtaattt cagagtataa taacattgaa gatgtggaaa agaaaaagaa
    35461 tgtgtacttt agcttattta tgtcactata taaattgaat agatttgaag atataaaaaa
    35521 ttattataaa gaagtttcaa attctaaaat tgaaaaaaat agtttttata gaaatataga
    35581 agaagttata aataatatta aagaggatga aaaagagtat gtatataaaa tcctttgtga
    35641 cattgaaggt aattatggca ttttaaatca aataaggata aataaatgtg tatctataga
    35701 taaatgtaaa gaaatactca atgaagaaaa ggaaataatt tatgcatata ttataaaaat
    35761 agcttttgaa gagaagattg atttactaga tattttttat gaaatggaat atgtatggat
    35821 agagaaatat ctaaaatatc ttttagcatt agaaagaagt tttaatttag tactatataa
    35881 gtacataatt agtcgtccaa atacatcaga tatggaaaag ataagagtat ataaagctat
    35941 atctaaagta gttttagaaa gtataagctt aagtgatgaa aaatataagg agatttttta
    36001 tctttatata atgtattctt ataagtacat aagatacata tatagcaatt taagtgattt
    36061 tgaattaata aaatttgttt gtaatgattt agataagttc actatagaat ttaagcatct
    36121 acttgatatt aaaaataaag taaataatgc taatgaaaaa ttagagtata tacatagtat
    36181 gaggaaatta ctaaatgagt atccttttta taataaaata ataaaaatac ttataggtga
    36241 attagaagaa aatataaaag aaagtgaaga atttaaggat ttaaaagata gttttattat
    36301 tagtatagaa aatatgattg aaactggcaa aataaatgat gcgaaattat tggtagatga
    36361 ctattcaaaa acatttgtgg atgatataaa gattttaaat ataaagggaa ttttatacat
    36421 gtttgaaaat aagcttgata aagctgattt tatgttaaaa aaggcattat cattagatgt
    36481 agaaaatgaa gatactatat ataatataga atacttaaaa agtttaagat aagtcaaaaa
    36541 taggagaatt gaagaatgaa tataccaata gtggttaatg aaaaatctaa agacataata
    36601 aaagattggc aatatacatg tacaaatgaa gatataaaat ttacaagcat aataatattg
    36661 acatataatc aaatagaata tactaaatta tgtattgaaa gtattaggaa atttacccca
    36721 aagaataaat atgaaataat aataattgat aataactctt ctgatggaac agttgagtgg
    36781 ttaaagggac aatctgatat aaaatctgtg tataataaca aaaacttagg atttccaaaa
    36841 ggatgcaatc aaggtattga gttagctact ggtgaaaaca tattgttgtt aaataatgat
    36901 gtaatagtta ctccaaattg gctctataat ttgaataaag ctttatggtc tcaagataat
    36961 ataggtgctg taggacctat tacgaatgca tgttcatatt atcagcaaat tgatgttgat
    37021 tataagtcaa ttgaggacat gtttatattt gcacacaaaa taaataaatc taatattaac
    37081 ctatggaaat atagattgaa gttagttgga ttttgcatgt taataaaaaa aactgttctg
    37141 gataatgtag gggttttaga tgaattattc acacctggaa attttgaaga tgatgattta
    37201 tcttttagga tgatagagtc aggatataag ttattattat gccaggatac ttttatacac
    37261 cattttggaa gtgtgtcttt taaagaaaat cgtaataaat atacagatat aatgtatatt
    37321 aatagaagaa aatttgaaac taagtgggga tttaatccag aatatagtgg aatgataaga
    37381 acagatttga tttctaaaat agatgatagt ttggaggcaa atattaatgt tttagaaata
    37441 ggatgcggta taggggctac tcttttagaa ataaaaaata tatataaaaa tgcatcaata
    37501 tatggaatag aaattagtga gtcagcaggt aaacttgcaa agtatagtgc agatgtactt
    37561 atagcaaata tagaaaaagt aaaattggat tataaaaaag atttttttga ttatataata
    37621 ttgggagatg tacttgaaca tttgattgac ccatggaaga tagtcaatga attgaaacaa
    37681 tatctaaaga aggatggtta tctggtatct agtataccaa atattatgca tgtttctgtt
    37741 ttaagaaatc ttgtgttagg taattttaca tatgaagatt caggaatttt ggacaagact
    37801 catttaaggt tttttacatt aaatgagata acaaaaatgt ttaatgatag taattacata
    37861 attaatagta ttacagctac tagtatgcca ctttcggaaa ttgataatag cttcattgat
    37921 acaatatgta aggaaagctg taaagaaatg agacaacagt acgaatctta tcaatatata
    37981 gtaaaagcct ctaaaatcct agatactaaa agatattcta gtattagtat gattaattta
    38041 aaatacgctt taatgagaat tgataatgaa ttagatgtag atagtaattt aagttatata
    38101 tttgataatt ataatataac tgaaccatgt tttgttgatg atattgaata tctaattgat
    38161 acatttgtga taaataaagc aactctatta aatagattag gagttgaggc atttaacaga
    38221 ggtctaggag attttagtat aaacatgttt atgacagtat tagagatgga tagagatgat
    38281 attgactcaa tatataatat aacaagtgta ttatgtgaat tagaagaatt tgatactgct
    38341 tataatttaa ttaaaaatag tagcgaaaaa gtacagagtg aaagtgatat acaagagata
    38401 ctttgtttta ttgaggggaa gaaatatgaa taatgataag atatgtttta taacctgtgt
    38461 aaatgatgaa aggctttatg aagaatgtct tgtatatata aatagcctag acattcctaa
    38521 aaactttagt atagaaacag tagctttaag aggagccgat tctattgcct ctgcttataa
    38581 taaagctatg agagaaacag attctaagta taaggtatac ttacatcaag atacttttat
    38641 aattaataaa aatttcatac atgatatttt agagatattt aaaaaaaata cagatgtcgg
    38701 aatgattggt gtagctggag taaaagatat gcctgtgaat ggaatttggt gggattctga
    38761 taaaaaagca gggaaagtat atgatagtca tacaggtaaa atggatattt atgaatttag
    38821 taactttgaa gaatgtttta atgaagttat ggctatagat ggattaatta tgattactca
    38881 atatgatgta ttttggagag aggataagtt tgatgggtgg catttttatg atttgtcaca
    38941 gagtatggag tttagacaaa ataaatacaa agttattgta ccaaaacaaa ataagccttg
    39001 gtgtatgcat gattctggag tagtaaatat aaataatgga tatgatgaat ataggataaa
    39061 atttgtaaaa gagtacataa atcttgattt atatagagaa aatttttatg aatttgataa
    39121 gaagaaagca agtaccatac ttaagagtaa taagcatgta atggattgta tgaaagagga
    39181 gcttacagaa caattaagat taaagaatta caaggatgtt ttaggtattg tttctaaaat
    39241 tgctcatttt tctgttttta atcatactag tttgtattct tgtgaagaat gtgagagagc
    39301 attgctagaa tgtgcaaata atttaccaga aatagaaaat acgaaattat ttacaaaaaa
    39361 agatttaaaa agaagaaaag tattacatgt tttaagtgaa ggatattcaa caggaggtca
    39421 tacaagacta gttaaaaatt ggattgaaat tgacaaagac tctgttcatt cagttgtaac
    39481 tacatggcaa tcagatacac tgccagactg gttattaaaa gcaataaatg atagtggtgg
    39541 cgaagttata tctttggatt taatttcaga tgattattta aaaagagctg tagctttgag
    39601 gaaaatagct tatgactggg ctgatatagt tattttatat gttcatatgt gggatacaat
    39661 acctgttgtt gcttttggag ttgatgggaa aatacctatt ttacatgtta atcactatga
    39721 tagtactttt tggttaggga caagtattgc agatattgtc attgatttta gcgaagctag
    39781 tcaattaatt acattagata aaagaggcat agataaatct gaaatattac caatgccatt
    39841 aaaagaaaaa gaagtatttt caaaatctga agttagaaaa cgatataatt tagaggatga
    39901 tgtgacaata atattgacta ttgctagtga aagaaaatac aatagtgtaa atgatattaa
    39961 ttactttaaa attgctaaaa cattgattga aaatacagat aatacaatta ttattgttgt
    40021 aggagttgat acagaggatc tctattggaa aactttaagt aatgttacag ataatagagt
    40081 tatatgtttt ggaacaacag caaatataga tgatttttat aagatttcgg atatatacct
    40141 agagtctttc atggtaggaa gtcatacttc aaaattagat gcaattttat atgatttaat
    40201 tcctgttaag tttaaaaatg aagtttcacc aatttttaca aatatgtgga attttataga
    40261 taagtttgat tataagaata taaaagaggt tatagacatg ataagaaggt acaatactag
    40321 tgaatttgac agggataaga taaaagcaaa acaatcggaa ataaaacatt ttgttataga
    40381 taaacattgt attgaaataa aaaactatat agacaaaata tataatgaag ttaatcatca
    40441 taaagttaag tatgatttat ataaaaatga tgaaatcgag gactatcagc tttttgtagc
    40501 attatatggc tatatggtaa aaaataaaaa catgtcatag gtggtaaaat gaacaatatt
    40561 catatcaata aatttgaaaa aagctgtgaa aaaaactggg attattttgt agaaaataaa
    40621 tctataaatg gtacatttct gcaaactaga aatttcctta attatcatga ggagggaaaa
    40681 ttcactgacc actcattaat aatatgtaaa ggtgaagaaa tactagcagt cgtacctgca
    40741 tgtgaaactt atgaagatgg tgaaagaata tttttttctc atttagggag tacttttggt
    40801 ggcattataa taagaagtgg ttttaatgat attaggcatg taaatgccat tacaaataag
    40861 ttagaaatat atttacagga acaaaatttc gataaagtat atttaaaaaa tacatcaaga
    40921 atattttgta aagaggatac ttcattacta gaatattttt tatttaagaa taattacatg
    40981 ttttatgatg aattaagtat gtttttagaa ttaagtaagt ataaagaaga tatattagcc
    41041 aattttaaat acagtaaaag aagagatttt aaatattctt tgaagaataa tattaccttt
    41101 aaaagactgt atagtgatga agatatagtt tgttttttta atttactttc taattgttta
    41161 gatagtaaag gagctaaagt aatacattca gtagaagaac ttttggagtt taaaaataat
    41221 agattaaaag atattgtaga attttatgca gtatatattg atgacattat gatttgtggg
    41281 agcatggtat ttaactttca agaaagagtt tatcatactc aatatctagc atcaaattcg
    41341 acatataata aatattatcc aatgatattt ttatactata atctaataaa agatgcttta
    41401 gagagaaatt ttaagtatct ttcttttgga attagcactc taaatagagg caaatactta
    41461 gatgagggtt tagctacatt taaagaaggt tttggttcaa gtgcagtttg taacagaaca
    41521 tactataaaa atataaataa gtgagaatca tgttttatag agtttttaga gtatagtaaa
    41581 tatctagtac tctaatatta cataaaaata attagtttat aaagaaggtt tgtatatgaa
    41641 aagattaatg attttaggct caattaagta ttttgagaat attgttatgt cagctaaaag
    41701 agagggaata tacacaattg tttgtgataa tagaattgat actcctgcga aaaagatttg
    41761 tgatgaagca atagatgttg atgtatttga ttttgataaa ataaagaata tagctataga
    41821 taaaaagata gatggaattt taactggatt tacagattct ttaatgaagc catatgttta
    41881 tattgcaaat gaactaaatt tgccatgtgt gatttcttgc aatcaacttg aatctgtaac
    41941 taataaggct gttatgaaaa attattttaa aaataatgga atacctacaa ctgattattg
    42001 tattattgaa agtttgaaag atttagaaaa aatacaagga ttaaattttc cacttgtaat
    42061 aaaacctgtt gatggttatg gttcaagagg tgtatatttt gtatcaaaca tagaagaatt
    42121 aatagaaaaa tttgaatatt ctaaggaagc ctcgcaagat ggacaagtaa taattgagga
    42181 attttatgaa agtgaggaaa tacaaggact agcttgggtt cataatggag agtcacatgt
    42241 attttatatt ggagatagag agcttgtaaa tatacataca ggaagagctg gaaaaccaga
    42301 tagattaatt tatccatcaa agtattgttt tcaatatgaa gaagaaataa aatccatata
    42361 tcaaaatata acgcaggctt ttggcataaa aaatggacct ctttatgtac aaatgttagt
    42421 gggagcatat ggaataaagg tatctgaagt aatgccaaga ttacctggag gttgtgacta
    42481 tatggctatt tctcaaataa cagatttaga cataggaagt ttatttgcaa actttagtgt
    42541 gaataatgaa gtaaattttg atgaaataaa gaaatataat atgcatctat caaaatgtgt
    42601 atatgcatta ccaatataca taagacctgg cacaataaaa aagataaata atatagataa
    42661 aatagaaaac ttagattatg tcactcaatt tctattacta gtaaaagaag gagatacaat
    42721 aggattatat gaagatgaga gacaggattg tggaagagtt tatggagtag caaataatgt
    42781 atttgatgca aatataaaga agaaatgtat tcatgaaatg atagaaatac ttgatgaaaa
    42841 taatgaaaat atgatagaaa atttttaaat aagggagtat gatagatatg aagctaataa
    42901 ctcatgaaca aattatgtca ttaaacattt ctcagaaaca aggatacgag tggataaaag
    42961 aatccttttt aacaaaaaaa gattgtcaat taccaccaaa aataagcatg gtagaagaag
    43021 ggcatgtatt ttacaatgta atgccgtccc taaatcataa tcataagatt ggtggaataa
    43081 aaatggttac aagattgcca gggagaaatc caagtttaaa ttcagagata tttctatata
    43141 atatggaaga tgctaaatta aaggctattt tagatggcaa ttatattaca gcttttagga
    43201 ctgctgctgt tgctgccttg gctatcgata catttgctaa aaaagatttt aatactattg
    43261 ctattatggg tttaggtgta actgcttctg ctataatgaa tatgctttca tatactctta
    43321 aagataaaaa tataaatata aagttactta aatataaaga ccaagcagag tcgtttgtaa
    43381 aaaagtatga aaaaaatgaa caatttagtt ttgaaatatg tgacacacat gaagaactta
    43441 ttgtagattc agatgtagtt atttctactg tgacatatac agatgaatat ttatcagaat
    43501 ttaaatggtt taaaaaaggt gctttattat tacctgtaca tgttatggga tttggaaatg
    43561 ttgattatct atttgataaa gtatatttgg atgattataa tcatttgaaa catctagaaa
    43621 atatagatag atataaatat tcttgtgaat tttatgatgt tttaagtaaa caagctaaag
    43681 gaagagaaaa tgatgatgag agaataatat cttacaatat aggtttgtca atacatgata
    43741 tattatttgc aaataaaatt tacgaattgt gtgaaacact aagctgtgaa gaaatagaat
    43801 taaaagaacc aaaagaaaaa tattggatat agtttatttc attcatattt acaatagttt
    43861 attgtaaata tgaatatgaa gtaatatatt gttagaaaat tagcatgaat aggatagata
    43921 aatttatgga taaatataat ttaattaggt tcgtatctat tggaaacgaa tatataggaa
    43981 atttagttgc attagaatca aataaagcaa taccttttga tataaagaga gtttattaca
    44041 tatatgatgt accaaacaat gtaaagagag gatttcatgc acacaaaaac ttggaacaag
    44101 tactagtttg cctaaatgga agtgtgaaga taaaatgtta tgatggtgta aaagagacta
    44161 tatttaattt acataaaaat gatgttggat tatatatagg aacgggagtg tggcatgaga
    44221 tgtttgattt tgatgaaaaa actgttttaa tttctatagc atctgaacat tataatgaag
    44281 aagattatat tagagactat aaagaatttt taaagtattt aaataatatg tagttattat
    44341 acagttagtg atgtattttc ttaattaaat aaatttaaga tagccacata gagaatagat
    44401 agattaggat gtgaatttat tgttggttaa atttaataat ttattaaaac agttccaaac
    44461 ccataaagaa gaatatgaac agaagattaa ggatgtaatg gaaagtggat attatatcct
    44521 tggaaatgaa gtaaagcatt ttgaagaaga atttgctaac tatgtaggta gcaaatattg
    44581 tgttggttta gccagtggtt tagatgcact gataattgct tttagagcat taggaataag
    44641 cgaaggtgat gaagtaatcg tgcaatcaaa tacatatatt gctagtgtta tgggaatcag
    44701 tataaatgga gcaacaccag tatttgtaga accaaatgaa tattataata tagattcatc
    44761 taagatagaa gaaaagataa atagcaaaac aaaagctata ttagtagtgc atttatatgg
    44821 tcaagcaagt aatatgaaag atatacaaga attatgtgat aagtataact tgtatttaat
    44881 tgaagattgt gctcaatctc atggagctaa atatgatgga aaaatgacag gaacatttgg
    44941 tgatataggt tgttttagtt tttatccaag taaaaatttg ggtgcatttg gagatgcagg
    45001 agctatagta actagtaatg aggaaatagc tgataaagtg aaaattctta gaaactatgg
    45061 ctcagaaaag cgatattata ataaatttgt cggatataat tcaagattgg acgaaattca
    45121 ggcaggttta ttaagagtaa aacttaaata tctaaatgaa attgaaattg aaaaatcaaa
    45181 aatcgtagat agatatctta atgaaattaa taatcccaaa gtcatacttc caaagataga
    45241 aaataattgt actcatgtat ggcatttatt tgttttacaa gttgaaaaac gagatacatt
    45301 tatggaatac ttaaagaaaa atggtataga gtctataata cattatccga taccaccaca
    45361 tctatctgaa gcatatgctt acttgggata taaaaagggg agttttaata tagctgagaa
    45421 ctattcagat aaagttgtta gtataccttt atttatcggt atggaagatg aagaagttag
    45481 ttatataatt aaaattataa atgaatatta aactactcat aataatatca aaaataaatt
    45541 ttttatatat catttattta agtttcgtta attg
//
LOCUS       DAEORZ010000015        42476 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEORZ010000015 DAEORZ010000000
VERSION     DAEORZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.015"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(130..324)
                     /locus_tag="KRH39_000743"
     CDS             complement(130..324)
                     /locus_tag="KRH39_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510667.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(633..2162)
                     /gene="lysS"
                     /locus_tag="KRH39_000744"
     CDS             complement(633..2162)
                     /gene="lysS"
                     /locus_tag="KRH39_000744"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBG8510668.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNSNKEEE
                     N"
     gene            complement(2177..2656)
                     /gene="greA"
                     /locus_tag="KRH39_000745"
     CDS             complement(2177..2656)
                     /gene="greA"
                     /locus_tag="KRH39_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBG8510669.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="KRH39_000746"
     CDS             complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="KRH39_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBG8510670.1"
                     /translation="MKIGNLKLKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYSGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3870..4640)
                     /locus_tag="KRH39_000747"
     CDS             complement(3870..4640)
                     /locus_tag="KRH39_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBG8510671.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCEDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4659..5249)
                     /locus_tag="KRH39_000748"
     CDS             complement(4659..5249)
                     /locus_tag="KRH39_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG8510672.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYMSGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5432..6400)
                     /locus_tag="KRH39_000749"
     CDS             complement(5432..6400)
                     /locus_tag="KRH39_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBG8510673.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLVVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKAIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIIKKREI"
     gene            complement(6393..7370)
                     /locus_tag="KRH39_000750"
     CDS             complement(6393..7370)
                     /locus_tag="KRH39_000750"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBG8510674.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLETIDSTNEHAKRTAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYIVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLPRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNKELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7685..9655)
                     /gene="ftsH"
                     /locus_tag="KRH39_000751"
     CDS             complement(7685..9655)
                     /gene="ftsH"
                     /locus_tag="KRH39_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG8510675.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEIE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9689..11077)
                     /gene="tilS"
                     /locus_tag="KRH39_000752"
     CDS             complement(9689..11077)
                     /gene="tilS"
                     /locus_tag="KRH39_000752"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBG8510676.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIVFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(11260..11688)
                     /locus_tag="KRH39_000753"
     CDS             complement(11260..11688)
                     /locus_tag="KRH39_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBG8510677.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNEVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11705..12511)
                     /locus_tag="KRH39_000754"
     CDS             complement(11705..12511)
                     /locus_tag="KRH39_000754"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBG8510678.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12713..13198)
                     /locus_tag="KRH39_000755"
     CDS             complement(12713..13198)
                     /locus_tag="KRH39_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBG8510679.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13249..13908)
                     /gene="spoIIR"
                     /locus_tag="KRH39_000756"
     CDS             complement(13249..13908)
                     /gene="spoIIR"
                     /locus_tag="KRH39_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBG8510680.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14053..14733)
                     /locus_tag="KRH39_000757"
     CDS             complement(14053..14733)
                     /locus_tag="KRH39_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8510681.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14717..15607)
                     /locus_tag="KRH39_000758"
     CDS             complement(14717..15607)
                     /locus_tag="KRH39_000758"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBG8510682.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15725..17275)
                     /locus_tag="KRH39_000759"
     CDS             complement(15725..17275)
                     /locus_tag="KRH39_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG8510683.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17369..17635)
                     /locus_tag="KRH39_000760"
     CDS             complement(17369..17635)
                     /locus_tag="KRH39_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBG8510684.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17783..18643)
                     /gene="yabG"
                     /locus_tag="KRH39_000761"
     CDS             complement(17783..18643)
                     /gene="yabG"
                     /locus_tag="KRH39_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBG8510685.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18727..19665)
                     /locus_tag="KRH39_000762"
     CDS             complement(18727..19665)
                     /locus_tag="KRH39_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBG8510686.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19775..19936)
                     /locus_tag="KRH39_000763"
     CDS             complement(19775..19936)
                     /locus_tag="KRH39_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510687.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19946..20443)
                     /locus_tag="KRH39_000764"
     CDS             complement(19946..20443)
                     /locus_tag="KRH39_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG8510688.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20558..21268)
                     /locus_tag="KRH39_000765"
     CDS             complement(20558..21268)
                     /locus_tag="KRH39_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBG8510689.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21444..21974)
                     /locus_tag="KRH39_000766"
     CDS             complement(21444..21974)
                     /locus_tag="KRH39_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510690.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(21991..22221)
                     /locus_tag="KRH39_000767"
     CDS             complement(21991..22221)
                     /locus_tag="KRH39_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510691.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22236..23630)
                     /locus_tag="KRH39_000768"
                     /pseudo
     CDS             complement(22236..23630)
                     /locus_tag="KRH39_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
     gene            complement(23756..25009)
                     /locus_tag="KRH39_000769"
     CDS             complement(23756..25009)
                     /locus_tag="KRH39_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8510692.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25060..26214)
                     /locus_tag="KRH39_000770"
     CDS             complement(25060..26214)
                     /locus_tag="KRH39_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG8510693.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(26774..27343)
                     /locus_tag="KRH39_000771"
     CDS             complement(26774..27343)
                     /locus_tag="KRH39_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG8510694.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            27546..27773
                     /locus_tag="KRH39_000772"
     CDS             27546..27773
                     /locus_tag="KRH39_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG8510695.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(27992..28735)
                     /locus_tag="KRH39_000773"
     CDS             complement(27992..28735)
                     /locus_tag="KRH39_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG8510696.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            28903..29775
                     /locus_tag="KRH39_000774"
     CDS             28903..29775
                     /locus_tag="KRH39_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG8510697.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            29775..30440
                     /locus_tag="KRH39_000775"
     CDS             29775..30440
                     /locus_tag="KRH39_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG8510698.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            30461..31318
                     /locus_tag="KRH39_000776"
     CDS             30461..31318
                     /locus_tag="KRH39_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8510699.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEKGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(31474..34020)
                     /locus_tag="KRH39_000777"
     CDS             complement(31474..34020)
                     /locus_tag="KRH39_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG8510700.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(34020..34706)
                     /locus_tag="KRH39_000778"
     CDS             complement(34020..34706)
                     /locus_tag="KRH39_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510701.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(34789..35778)
                     /locus_tag="KRH39_000779"
     CDS             complement(34789..35778)
                     /locus_tag="KRH39_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8510702.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(35765..36451)
                     /locus_tag="KRH39_000780"
     CDS             complement(35765..36451)
                     /locus_tag="KRH39_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8510703.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(36951..40157)
                     /gene="carB"
                     /locus_tag="KRH39_000781"
     CDS             complement(36951..40157)
                     /gene="carB"
                     /locus_tag="KRH39_000781"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG8510704.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYSTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(40337..41386)
                     /gene="carA"
                     /locus_tag="KRH39_000782"
     CDS             complement(40337..41386)
                     /gene="carA"
                     /locus_tag="KRH39_000782"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG8510705.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(41643..>42476)
                     /locus_tag="KRH39_000783"
     CDS             complement(41643..>42476)
                     /locus_tag="KRH39_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBG8510706.1"
                     /translation="SQNISDEIKEKIVEYTKKIALDLNVIGMVNIQFIEFQNELYIIE
                     VNPRASRTVPYISKVSGVPIVDLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSM
                     SKLSKVEVSLGPEMKSTGEVLGVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDK
                     DEFIEIAKDMKELGYTFVATEGTAKSLRENGIEADIVNRVEEPRPNILDAIRNKQVDI
                     VINTPTKGNDSTRDGFKMRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIA
                     E"
BASE COUNT        13398 a         7948 c         4711 g        16419 t
ORIGIN      
        1 tttcgtcaat actttttttg ttttttattt taaaaaaggt tataactttt ggttttttta
       61 ataaatataa ttaaaactaa tagatataac ccttgatatt acaaacaata catgcatata
      121 taattttacc tattttgcaa ttttattttc ataagaatat gaattagctc tcactctatc
      181 tgcccaaaca tttatactct taactttatt aacattagtt gtaccctcaa aaatatccat
      241 tgcatcctta caaaaatata tcataacatc aagaaccaaa aatccaacac aaaataaaca
      301 taaaaaatta attacttgaa acatcagaca ttcccccaat aattttttat atatagtatt
      361 ctcataaatt attcttttta ccttctcata aattattcta aatattttca taataaatta
      421 ctttcaatca tattttctaa atataaagtc tcaatcaaca aaaactaata tcaatttcta
      481 acgtttgttg gtaatttata aattaaatat ctaaaagaat ctttgaagca tataaaaagc
      541 attctacact gacattttaa ataaaattta gctatttatt gataatttaa tagctaaact
      601 ttatttaaaa cttagtagaa tgctaatatt tcttagtttt cttcttcttt atttgaattg
      661 ttatcaatag gtttcatagt cgggaataat aatacatctc ttatagatgg agagttagtt
      721 aataacataa ttactctatc tatacctatt cctagtcctc ctgtaggagg tagacctacc
      781 tctaatgctt ttaagaaatc ttcatccatt tcaaaagctt catcatcacc taattctctt
      841 tttcttaatt ggtctataaa tcttcctttt tggtcaatag ggtcattaag ttcagaaaat
      901 gcattagcta actcccactt attagcaaat gcctcaaatc tatcagttct tcttgggtct
      961 tcaacatttc tcttagataa tggagatact tcaactggat gatgagttat aaatgttggt
     1021 tggattaatt tatcttctcc aaattcttca aagaaagcat ttattacttc tcctcttctc
     1081 attccaggtt ttatctctat acctttttct ctagcaactt ctagtgcctc ttcatctgta
     1141 tttatagtag aaaaatctac accagaatat tctttaacac aatcttccat actcattctt
     1201 ttccaaggtg gtgtaaagtt tatttctgtt ccttgatagt taactatcat acttcctgta
     1261 gcaacttcag ccatatgtgc aataacattt tctgttattt ccatcatatc agtatagtct
     1321 gcatatgctt gatataattc tattgcagta tattcaggat tatgttttaa atccatacct
     1381 tcatttctaa acattcttcc catttcataa actttatcaa atccaccaac aataagtctt
     1441 tttaaataca attcattcgc tattcttaaa tacattggta tatgtaaagt attatgatta
     1501 gttataaatg gtctagcatt tgccccacct gctatagtat ttaatattgg agtctcaact
     1561 tctaaaaatc ctctatcatc taagtatgct cttaatgctt ttaatgcttt tgttctaatt
     1621 aaaaaagcat tttttacttc tggatttact attaagtcaa catatctttg tctatatctt
     1681 agttcttggt cttttaatcc atgatatttt tctggtaaaa cttgtaaaga tttacatagt
     1741 aatactacag atttagcctt tacagatatt tcaccttttt tagttttaaa gatttctcct
     1801 tcaattccaa ctatatctcc aatatcatat gttgaaaata cactatattc ttcctctcct
     1861 atagcatcta gtcttacata agattggatt cttccctctt ggtcttgaat atctataaag
     1921 ccagctttcc cttgtattct cttgctcatt atacgtcctg ctatttttgt agttttacct
     1981 tcaagagaat caaagttatc ttttatatcc atagaataat gagttctatc atatctactt
     2041 tctttaaaag gatctctacc tgactcttgt aattttttta gtttatctct tcttacttga
     2101 agtacttcac taaggtcttc ttgaatttgt gcttcttcat tgctttgttg attatttttc
     2161 atttttttcc tccaaattat cttcttattt ctaatatttc aaacttagca actccatcag
     2221 gaacttgcac ttccacaact tctttcgcag ctctacctaa aagtgctttt cctacaggag
     2281 attcgtttga tattttacca tcatatgggt ctgcttctgt cgaaccaact atagtatatt
     2341 caacctcttc atcaaattct aaatccttaa ctttaactat tgatcctatt gtaacaacat
     2401 taaggtctat tttgctttca tctatgataa cagcttttct aaccatattt tctaatttta
     2461 aaattctttc ttcaacttgt gcttgttcct ttttagcttc atcatactca gcattttcag
     2521 ataaatcacc aaaagatata gcaactttta ttctctctgc aacttctttt ctctttacaa
     2581 cctttaagtt ttctaattct tcttctaatt tatcatatcc ctcttgtgtc aataaaaatt
     2641 ctttattttc ttccattata aatacaactc cttcacaaat ttcttaggaa ttttcctaat
     2701 tatattttaa aaattttata atcttttttt aatttatgcg ctatattata agtcactatt
     2761 atttttatgt caaggagtct tccatatagt actccaattt cgacacaact tcttgatagt
     2821 cttctatttt gttaatttca tcccttaatt ttgcagagtt tctaagtcct tttaaatacc
     2881 aagcgatgtg ttttctcatt tcccttacag ctacatactc tccatgttct tctactgcaa
     2941 gttttaaatg cttaatggca gtacttattt tttcgttaag agttggctct ggcaaaatct
     3001 cgcctgtatt catataatgg tttattcttt taaatatcca agggttacct tgagcacccc
     3061 tacctatcat tatagcatca caaccagttt tgtctaacat atttattgaa tcttctattg
     3121 taaatacatc cccatttcct ataacaggta tactcaaatt tttcttaatc tctgcaatta
     3181 tatcccagtc tgctttacct gaataaaact gttctctagt tcttccatgt attgctaaag
     3241 cgcttattcc acaatcctct gctatctttg ctatttcaac agcatttaca ctattatcat
     3301 cccaaccttt tcgaatcttt aaagtcactg gcttttttga gttcttcact actgccctca
     3361 atacctcttc tgcaagctta ggatttttca taagtgctga accatctcca tttttaacta
     3421 cttttggagc tggacacccc atatttatat cgagaatttc atttgaataa tcattcatta
     3481 tctctgctgc tcttcccata aactctggct ctgagccgaa tatctgaact gctacaggat
     3541 gttcttcctc ttctatctta agcatttttt tagtattttc atcatcatag caaagtgcct
     3601 taccatttat catttctgta tataaaagcc cacatccctg ttctttacaa attaatctaa
     3661 atggaaggtc tgttacacct gccataggtg atagaaaaac tttatttttc aattttaaat
     3721 ttcctatttt cattgttttt ctcctatttt tgcctttatt ttgtgattta actttaaatt
     3781 cttatttcct cattataata taccacatat atctagaaat ctatcataaa aagggaattg
     3841 gatatataac ccaattccct gtatattaat tattcttggt ttttttcata tattattctc
     3901 aatccttcta gtgttaaaaa accatctaca taatctatac ttttcgtctc tgaagcaatc
     3961 agtttagcta atccacctgt agctataacc ttgacatcct cacaattcaa ttctttcttc
     4021 atcatactta ttattttgtc aactaaacca acatatccat aaattattcc agattgcata
     4081 gctgatacag tactcttaca aatagtcata cctggcttag ctaattctac tctaggtaat
     4141 ttagacgcac tttgaaataa cgcctcacta gatattttta ttcctggtgc tattgttcca
     4201 cccaaatatt cacctttttc agagatagca caaaatgtag ttgctgttcc aaaatcaaca
     4261 agtatacttg gtgctccata cttttctatc cctgctacag cattaactat tctgtctgcc
     4321 ccaacttgtt ttggattatc atattttata tttagacctg tttttatgcc tggacctact
     4381 attaatggct gttttttaca gtattttata caaaagtttt caagagaatg cataacattt
     4441 ggaactacag atgatattat aacatcatca atgtcactta tattcacatt atcataatca
     4501 aataggttac ttatcaggat tccatattca tcagacgttt tttctctatc tgttttaatt
     4561 ctccagtaat taactaattt gtcaccttta tatataccta aaaccatatt agtatttcca
     4621 acatcaaata ctagaagcat atgtcattaa ccttctttct attttttgct tttcttaagt
     4681 acagtggcaa cagcagctac tactaaagcc cctactattg cttctggcac tccatttgct
     4741 gcggcaagag ccattattac ttttcctgct gaaccacttt tacccattgc atgaagatac
     4801 ttgtcagcat ataagacata tatcattcct aaaactccag ctgtatttaa cagagaacct
     4861 attgcccctg taattaaacc tgacatgtat accttatttg tagccttaat tattaactga
     4921 tatacataat aagctaatac acctatcata actcttggta atattgaaac taatggattt
     4981 ataaaaacaa aagatgttac tgttgggttt gttatagcat ttaagagact tgatatccca
     5041 aatataaatc ctattgcagc cccaactatt ggtccttcca taatagctcc aataataact
     5101 ggtatgtgca ttatagttgc ttttgtaggt ccaactggaa taaatcccaa tggagtcata
     5161 gatagcataa tagaaatagc tgatagtact cctattatag tcatctttct tacatttact
     5221 tttcttactg atgcacttgt gttcatcata aaaaatacac ctccgttcca gctcattaag
     5281 atgccggact acttaatttt ttaatttttt cgtttttttc ttataattat gagtcaagct
     5341 accattgtag gatgcccgct ctaacaagaa taattttatc aagatgagtt gccattgtca
     5401 acaatttgac aagaaaaata tgctaatttc atcatatttc tcgttttttt attatatctt
     5461 tatatgccat tatattttct acactatccg ctttttttac tgcatttatt atactttttt
     5521 ccacaacctc tacagccaat gaacctacta atgttatatc tgcttctatt tctcctgttg
     5581 atattgtgaa tattgtatct ccatcatgag gtgtatggat aggaaaaata gctttagcat
     5641 atccatcatg tcccatttga gatatctttt tacatccagc tttggttaac ttagcatttg
     5701 ttgcaattat cccaattgta gtattatcta cactaaatcc acctttactt tcaccttgtt
     5761 tcattaattt atatgtatca attgtttctg tctttttatc atttaaagca cctgctataa
     5821 ctttaccatt ttcataaaca tctccaagag catttacagc aattaatgca gatacaacta
     5881 gaccattatc taattttata gaatagctac ctattccgcc tttcatagca tattcctgac
     5941 ctcttatctt tccaacagtg gctccacaac ctgctccata atttccttga tttaaaatat
     6001 cagtatttgc aacttcacaa gctctataac ccatctttaa atctggtcta catttaaagc
     6061 tccctactcc taaatcaaat aaaactgctc ctacaactat aggcacttta cctacaccta
     6121 catcaaatcc aattcctttt tcttctaagt atctacttac accacaagtg gattcaagac
     6181 catatgcaga acctccagcc aaaactactg catgaacttt ttggaccata tttacagggt
     6241 ctaataaatc ggtttctcta gttcctggag cagaccccct tacatcaaca ccgcaagtag
     6301 ctccttcttc acatattact actgtgcacc cagtaagacc atttctatcc tcaacctgcc
     6361 caacctttaa gccttctata tccaaaatat tattatacat atcctttttc tcctcttata
     6421 gaaacttcac ctgaaataat ttcttttata cttccatcac ttctttgaac taataaattc
     6481 ccctcttcat ttatgtctaa gcatttcact agttctttat tatctccttt taatacatat
     6541 atatttttat ctataattgc agaataatct ctacatattt ttaaggtttt ttctttggaa
     6601 ttatttacag tataatctaa atataatttt tcaaattcag tcaatacatt cctaacaata
     6661 tctactctgg gtaacatata tccttcttta tacaatgatg tagctttgtc agctatttct
     6721 tgttcaaaat ccattatttt aacatttatt ccaattccaa gaacaatgta gtttgttctt
     6781 tcaatttcag cagataactc tgttaatata ccacatattt tttttccatt taaaataaca
     6841 tcatttggcc attttattaa tgtatttgct cctagattgt ttaaagcttt cgttacactt
     6901 gctcctgcta ttaaagttat aaatggagct ttgaatggtt ccatatctgg ttttaatata
     6961 atactcatcc agattccttc atgaggtttt gaatcccatc ttcttcccaa tcgtcctttc
     7021 cccatatctt gatgttcact taaaatcaca gtcccatcta cactatttat agctgtcctt
     7081 ttagcatgtt catttgttga atctatagtt tcaagatgtt caatattttt tccaataaat
     7141 tctgtatcca atttatagta aatattctgt gggcttaata aatcagttgg attttccgct
     7201 aatcgatacc ctttcttatt tacagattct atattatatc cctcttcttt taaggctttc
     7261 atatgtttcc atatagctgc acgtgacact cctaattgtt ttgataattc ttctcctgaa
     7321 ataaattcag atttattatt taatataatc tctattattt tatctcgcat atttcacctc
     7381 taatatgaca tgatttcatg acttattttc ttctaataaa taattataca ttattaatat
     7441 acttttaata ctattaattt taaatttcct ttttaattat tataatggtt gttttatatt
     7501 ttatattgat gatttatatg attttacatt ttatctaaat aattttaaaa taataaaaaa
     7561 actgaaagta tcaattaaat taatgcatgc aaatatactt tgcttattaa tttaatatta
     7621 tactttcagt tttagtattt aatccacatc agataataat cttaaaatga ccaaggtctt
     7681 aaaactattt atctttatca gatttatctt ctaaattttt atttatatca ataacattgt
     7741 ttctttcttc ttcatctttt tctaatttta tagttaattg ctcttcaatt tctttagaag
     7801 aattttcctc tgtaactgca ttttcaattt cattcagagg taattcttta ttaaatgctt
     7861 ttacaaattg ttctgcatct aaagtttcat atataagtaa agcttgtgct acatactcta
     7921 atctatccat attttcttgt aatatacttc ttgtcttatc atatgcacca tcaactatac
     7981 gctttgtttc ttggtctatc tcaaaagcaa cttcttcaga ataatttctc ttacttgaaa
     8041 aactattacc taggaatact tcatcatcac tgtcaaatga cattggacca agtttagaac
     8101 tcattccata ctttgttacc atacttcttg cagtagctgt aactctttct aagtcatttg
     8161 aagcacctgt agatacatcc ttaagtacta gctcttctgc tactctacca ccaagtaaaa
     8221 ctacaatatt ttctttcatt tcattttttg tagcatagaa cttatcctca acaggaagtt
     8281 gcattgtaaa ccctccagct cttcctcttg gaactattgt aacttgatga acaggactta
     8341 cttcttcaag tacatgggca caaactgcat gccctccttc atggtatgct gtaagtcttc
     8401 tttctttctc acttattact ttcgattttt tagccacacc tgctataacc tttgttatag
     8461 cttcttctat agtttccatc ttaattttct tttctctttt tctcgctgtt aatattgcag
     8521 cctcattcat taagttctct atatcagcag gagtaaatcc tggtgttctt ctagccaaga
     8581 catccatttt tacatcatca cttaatggtt tatttcttga gtgaacttta aatatagcct
     8641 ctctaccttt tacatctggt gtaccaacaa caacttgtct gtcaaatctt cctggtctaa
     8701 gtaaagctgg gtcaagtata tctggtctat ttgtagctgc catgattatt ataccttgat
     8761 ttaccccaaa tccatccatt tcaactagaa gttgattaag agtttgctct ctttcatcat
     8821 gacctccacc aagacctgca cctctttttc taccaactgc atcaatttca tctatgaata
     8881 tgatagctgg agcacttttc ttagcctgtt caaataaatc tcttactctt gaagcaccaa
     8941 ctccaacaaa catctcaacg aaatcagaac cacttatact gaaaaaggga actcctgctt
     9001 ctcctgcaac tgctcttgat agatatgttt taccagttcc aggaggtcct accataagca
     9061 ttccttttgg tattcttgca cccaattcaa tatatttctt aggattcttt aagaaatcta
     9121 ctacctcctg caaatcttct ttttcctcat ctagtccagc tacatcttta aatgtaactc
     9181 tagttttttc atcatcttta tgaaccttag cttttgattt gccaaaactc ataacctttc
     9241 cgcctccacc ttgagactga ttcataaaca cgaaccaaat aatcaccatg aagaatatta
     9301 acagtaatgt aggcaacatt tcgacaaacc aaggtgtaga tggcttagct tcaccaccaa
     9361 aagttagttt cccagcctta gcttggtcta acacctcatc tgcaagttta tttcccatca
     9421 cttctgttgg aacatatgac ttaaactttg tatttgtatc ttttatagta ccttctacag
     9481 aagtttgatt tacaaagtat aatcttgata tattctcatc tgttaattct ctatatactt
     9541 cagagaattt taaatctttt attttttctg taggcttacc tgagaattga actataccta
     9601 ctataattat aaaaacaagt agataaaaac ctgccccttt tagcaactta ttcaatatag
     9661 aagccctcct ttcatacatt gctttgccct ataatttgtt aaaattaatt tttaatactt
     9721 cttttgtgtt ttcatcaatt ttataatttt cactgatttt ataatctcca acacatatta
     9781 ttccttcact atctgtgatt ataggaattt tacatctttc ttctcttggg atttttaaat
     9841 ctatgaataa atctttcact tttttacttc ccatagcaag ctttatctta tcaccttgcc
     9901 ttctgctcct tattactata ccccctttta ccttattaaa atcaaaccct ttagatgaat
     9961 tgtctaattt catactctta tacctatcta tgctcattac ttgggtctca ataatttgtt
    10021 ttaattcttt tattttaata aacccattac taggtacatt gtaataaaat tcaatttcct
    10081 cattaacaat ttcctcattc gttaatatta tactgtcttt ttttctataa acaaatatat
    10141 ttcttggaag agttaacatt ttatttactt ttgaattatc ttctaaagac attatatcct
    10201 ctatatgtct ttggtcaaca aaattagtat ctcctagtat atgttttatg gaatttctaa
    10261 gaactctaac tttgatagca tcatgtaagc aaacaaaatc gtctaaattt atctctacaa
    10321 agcctttttc ttttatattt gaaacttctc taaatttagc ttctgcctct ttctcaatat
    10381 aatcgttatc actttttaaa ctattgctca tcctcactat agattctatt acatttgaat
    10441 taaaattatc cttcatatat ggtaaaaggt ctaaccttat cttatttcta gtgtatatat
    10501 tttctaaatt tgttttatct atcctcggat ttaaattata agcttcacaa tactcctcta
    10561 tctcatttct ttccacatct aatatgggtc taattatgca attatctcta atataatcaa
    10621 ttccctttaa accttttaaa cctgttcctc tcatgatacg catcataaca gtttcggctt
    10681 ggtcattgag attatgacct attgctattt tattggcttt aatcttatcc ttaatttcgt
    10741 aaaacatttc atatcttagc ttccttgctc cttcttctaa tgataatccc tcattttcac
    10801 aatattttgg tacattaata gactttacaa agaatacaat tcccatatcc tcacaaagtt
    10861 tagatacata taatgcatct ttttgagctt ctatccctct tatttgatgg tttaaatgtg
    10921 ctgcatatat ttctatatta aaatcttttt ttaatctatt tagtacatgc aataaacaaa
    10981 ctgaatcagg tcctccagaa agacctaaaa ctattttatc gcctttttgt attaaattat
    11041 gtttatttat agtacttagt accttatcaa atatcatatt tatccctctt acacttagtt
    11101 ttattcactt ttaagtatac tttctataaa aaaacgctaa acccttgtat caattttaag
    11161 aaattaaagt ttaatataaa tataataata ccaaataaat taaataaaag gtagtttttt
    11221 cactaccttt ataaaatttc tggaaaatag ccatagttat tatttagtac ctatgtatat
    11281 atctatttta ttcctaccta aaaataaatc ttgtaccttt atattatagt ctattttcaa
    11341 ttttatgtgt tccccttctt gtatatttat atccaattct cttgtatcaa tatcaattaa
    11401 taaagtacct tgtggggtca catactttgt agtactactt tctccacatt taaacaacat
    11461 tgttgagtta atagcaccaa actttttgat actaacttca tttttaagta tctttatagt
    11521 attagttatt tctatatttt cttctttttc cttgtaaata acatatattc catcattttt
    11581 ataaaaaatt ttaccaaagg cagtcatctc tatggtgtcc atatttcctt tttcatcata
    11641 ttgtctagta tttatactta tttttacact caacgatttt tcctccatag attcttcata
    11701 taaactagta tttttgtatt tctacttttt gaatcttatc aatttttttc tttaagaact
    11761 ctcttgctat atccgaaaat ttttcaggta tatctgatac ataatattga taagttccat
    11821 gaatttctgt attgtttata atattttgca cctctaaaat cttttttaaa tcctttgcag
    11881 tctctttagc tggattaaca agttttatat gctctccaac ttcttcacct attgttctct
    11941 ttaaaatagg ataatgagta catcctaaca ctaaagaatc tatatttttt tctttcaatt
    12001 cttgtaaata tatctttgct gtcaatttag ccacttctgt atttgcccat ccttcttcaa
    12061 caataggcac aaataaagga catgctttat taacaatttc tatactctca tctattttag
    12121 atatttccaa gttgtatgct tttgaactta tagttccttc agttcctatt attcctacaa
    12181 ttttgttttt tgtagaactt acagctgttc tagctcctgc ttctataact ccaattatcg
    12241 gtatatcata tttttcttga gcttctttta agcttcttgc agtagcagtg ttacatgcta
    12301 taactatagc ttttacacct tttgttttta aaaagtttat agcttgaaaa gtatatttaa
    12361 ttatagtttc tttggatctt gaaccatatg gtattctggc agtatctcca aagtatatta
    12421 tatcctcatt tggtaatatc ttcattatct cttttagaac tgttaatcca cctaatcccg
    12481 aatcaaaaac tcctattggt ttattgttca tctttataca acacctactt tacatttttg
    12541 tgtttaacaa caacttattt atcgctaaaa tttatattta tattcataat taatatatta
    12601 ttacctaaat gattattata gtttatttta cacaaaaaat aaaggagcta tcttaaatta
    12661 gaaaataatt tctacaaaaa gacaactcct ttcactactt aaaaatttaa atttattttg
    12721 caaacacatg atttcctatt ctcatataag gattcaatct atttatccat ttgcaagtgg
    12781 ctatttctgg attccaaaaa tatactgctt tattagttgg gtcagttcca taaagagctt
    12841 cctgagctgc tttacgtgct gagtctgttg gtgccatctt tattgaacca tcaagcacaa
    12901 ctgagaaagc atttttttga tatatgactt cttctattgt gtctggaaat ctagggtcca
    12961 atactctatt tattactaca gctgctacag caacctgtcc ctcatagctt tctcctctag
    13021 cttctcctgc aaccaactta gataataaca tcaagtcatt atcagataaa tttattactt
    13081 ctttctttgc tatatttttt tcttgtttgt ttactttctt aatattttct gttggttcat
    13141 atgcaaatac ctctttaata ggcataaata taaaagtcaa agctaatagt gtagccatca
    13201 gtttactcca cctgtccatg attaaaacca ctccttacat tcttattttt aaaatttttt
    13261 ctctaaatcc ttgactactt caagtacttt aaacttaatt tttactctac ttgttttttt
    13321 tggtgtatct tgttttatta acttatattc tttatcagtt aaaacttcct tcaacttatc
    13381 atctgtagat ttatctatca ctccattaga ttcatcaaca aaacatagtg gagggaacat
    13441 aacacaccac cagttttttc cttcaccttt cccaatgata atctttaatg ctttatattc
    13501 accagctgga agaactatat tagaatattg tttagtaggg aaattgctat attgtatacc
    13561 tacctttaca ctgtaattat atccattttt aagaataata tttttactta tttcttccag
    13621 actactatat tcctttttta taatagcttc actttctttt atactttttg aattttgaag
    13681 ctttggctgt aaatatttta ttacttcatc tctaactttt agtttaagtt cttggtcttc
    13741 atctgtattg ctgtttgcta taacatgaaa tcttattaat ttatctttat aatcttttga
    13801 tatattgtct acttttttta cctcaccatt tattactatt gtcattattg atatgactga
    13861 tataagactt aatattaata gtcccaatct aatcttaatt tttctcattg aaaattcccc
    13921 ctcaagataa atttatatca tcaattatgt ccaattaaat ttaatttatt cataatattt
    13981 tttaatacta ataaaaaaag ctatcttaaa atgattacaa tcattttaaa atagcttttt
    14041 aaaccttata ttttataagt ctttactaat catagcctta tctatcataa actgctctgc
    14101 tttttctata tgctctgtag catacttctt ggatagctca ctatcacctt taattatagc
    14161 atcaagtaag tatttatgct cttcaacaat attatgtgat gtatcataag cagacatata
    14221 ggtaactcta aatctatata cttgttccca taatgagttt attagttgga ttaatttttt
    14281 attattagtt gctttaaata tacattcatg aaattctaca tcaagtttta ataacgcatc
    14341 tacatcggat tccaaagtgc tttttttaaa ttcttctgat atatctttaa gctttttaat
    14401 atcttcatca ctaattcttt cagcggcaag atatgctgct aagccttcta ggcttgctct
    14461 tacttctaat acatccataa tatcctttaa tgacatattt gccacatacg caccttttct
    14521 aggcaacata acgactaaac cttctaattc aagctttctt attgcctctc tcactggagt
    14581 cctacttact cctaattgtt ctgctagttg cacttccatt agcctttgac caggctttaa
    14641 ttttccttct aagattgctt ctcttaaatt ctcaaagact acatctctta atggcttgta
    14701 attatccaag ttcagtttag ttaaattctc cacaaatttc aacccctttt tgactgctat
    14761 taacaacata cacctgttta tattttttta ataattcttt ttttccaatt agagcatctt
    14821 ctttattttt aaatagtcca aaaactgtag gaccacttcc actcatcata gaacctaaag
    14881 cattattttt catcattact tgttttatat cacttatttc tttgtgttta cctatagtaa
    14941 cattttcaag aatatttacc atgctttctg atacagcttt tatatcttca gattttaaac
    15001 actctatcaa atatttatta tttggtcttt ttttaatatt attcaaatct aatccttgat
    15061 aaacttcttt agtagaaacg aataagtctg gtttgcatat caatatattt gtatcacatg
    15121 gtaaaccttt gatgtttgtt agcttttctc caataccttg agctaaggca ggtcttcctg
    15181 atatacaaaa aggaacatct gcgcccaaat taagtcctat ttcttttaat tcatcttcag
    15241 ataatctaag ttcccatagg tgatttaaac ctactaatac tgcggctgca ttagaacttc
    15301 ctccagccat ccctgctgct acaggaatat ttttttctat gaatatttct acaccttttt
    15361 ttatataaaa tttattttta agtattttgg cagctttata aacaatatta tcttcatcta
    15421 aaggaatatc taaactagtg ctttttacct ttatttcatc ttcgtctaat tcttttatct
    15481 ttactatatc gtataaatct atcgtttgca ttatcatttc aacaaaatga taaccgtctt
    15541 gcctttttcc caagacatca atggataaat ttatttttgc tctactcttt agtcttataa
    15601 aatccattct atcctccttg tttaaagaat aatgcatcac taacaataat attatactac
    15661 aaaaaataaa ataatagggt attataatac cctattatac ttaattactt taaatttttt
    15721 aatattaatc tactaatacg actttttttt ctaaaataag acacttacca ggcttaattg
    15781 gttcatcaag ttttatatca ttaagttctg caatttcatt ttctgttgta ttgtattttt
    15841 ttgctatatt ccagaaagta tctccttctt tgcatatata tacgattatg ctaggagctt
    15901 tagataaatc atatacacct tggtcttctc ccttgataat aaaattttct gctttcttat
    15961 ctaatgcttc agtaaatctc ttaattttga taatcaagtc tatctggtcc ctattcaaat
    16021 caacttcgac cttatcaata catgctgtat taaacacaga tgctgtatca gttagattat
    16081 ccatttctat atcatgttca aatggaattt cctcactaat tttataaacc aattttaaac
    16141 cttcaactgg gacaaataaa atttcaactt ttatgattcc ttgtataatg cttttatttc
    16201 cttcaatata actattttct atagacatgg ttggacaaac gcttacaata tcttttattt
    16261 gtatatcatc attatcattt cttatgcctt ctctaaccat aaatgtttct tcactacgtc
    16321 ttaaagtctt atttaattga ataggtttgt ggtcaaattt tattatcttt tgaggcgaat
    16381 atgcatcttg taatacttct cttgtaactt catctgtaac ttttacctta caacaagcca
    16441 tacaatctat ttctaaaaga cctgtgttgc tttcactatt ttgcttgaaa atatgattaa
    16501 aatctgacat agacaacaat acttcttcag tcataccatc actaactcca gggacttcta
    16561 caaattgagt aaactcaata cctactctat ccaactctac aagttcaccc tcatatgtac
    16621 aagctaacgg atttatttct aatactccac caataataac tttattgtca gtcactctac
    16681 tttcttttac tttaacacat ggatttaaac ttattataga ttgaatttcc tctgtgttta
    16741 ttgttatagt atctcttata gaactttctg ctttttctat tccaactata tcttgaaaac
    16801 aaatttcctt tctgtgcttt tgtatgcctt ccacttgcgc cacatctttt acaatgtcta
    16861 acctttgctt ttcaaacaaa cttcccctta tattcataag agctcctact ttaatctttc
    16921 tctcattcat tatagtacaa tccatatgct ctacttctga aaataacatg tattccatat
    16981 cttgaataac attgtctttt tctattactt cgtttatatc tacttttccg tctacattag
    17041 aaatagtatt tttatcatct gcaatataga ttacattata attaaaacta cctctgcaaa
    17101 gtatcttccc atctgccatt tcaatttttt taagtgatat gtatccttct gtttttacaa
    17161 tttcatatac atctaatttt ttgtctggta caactgcctc cgcttcaatg aatgtttgaa
    17221 attttccaaa gtctattcta ttgtcaactt taattacatc tttaattaat tccatagtct
    17281 acctccttaa gtattataaa aatatcacac tatatttata tttaaggagc aaaaaaaaaa
    17341 ttacacctat ttttaaatag gtataatttt aacttatttg taatttatct tgatctctaa
    17401 aaacttgcaa ctttacatta gatgttaata aatctgagta actataagat actctaggat
    17461 atccattact ttcatcatct aactttataa caaacacgct tggataaact ttttctagta
    17521 taccctcttt tgtaataatc tgctttcttc ctttattagc tttcaacaat attttctttc
    17581 ctatatgtct ctctaagctt actcttatct tatctagagt ttgaacagta gccacaatat
    17641 caccttcttt aacgtttata tgtttacata atatcacata taaacatttt tgtcaaatgt
    17701 ttaaactaat tattttatat attttttgca aaaatgtcaa tactattttt gaagatatac
    17761 agtataatta tgttaatttt tgctaatgta atattgtttt tggcattgcc catctaaatt
    17821 ttcctcttgt ttcagaacca ccaattccct taacacctgt aatagtctgc tctataacct
    17881 catcaatagg aactactaca tcaattcttg aacaagcaat tttctccact ataaaaacag
    17941 ggtctaatgc atgtatcatt attcttccag gcgaacttgc atagtttgct cctgcactta
    18001 ttattttttc ataatttgat tggcatgctc ctgcgaatat gacaagatta tccaaatttg
    18061 gttgccattt acgtgcttct tttactgttt taacaaaatt tgaagagttt ctatagttac
    18121 tcatatcttc tatatctcct ctttttattg tcattgcatc atgacctgtt attactagta
    18181 tatccggatt atatttttct agtagtgctc ttacttcttt atactgattt tgctctgaaa
    18241 cagctacacc tactgcaggt attccaagtt gagcatatac atccaagcaa atttttaaat
    18301 attctttatc tccatctatc tgtagtactt ttccaggcat tccatatgtg ttaggattta
    18361 attgaagttt tggaactgct ctagtcattc ttttttgtct ttctttagct tttcttactg
    18421 acttatataa taaattttct acatttttat ctattaatat atcttttata tcaggtgctt
    18481 ttactaattc taggtcatca atataagcat ctgctattac tctaaatgca attcccttta
    18541 gtatagctat tttctcatta ttttcatcta caccaaaaga gactatttta aatactatat
    18601 ctttgttatg tgactttcta gctacaatgt ctcctacctt catagttaaa cctccaataa
    18661 atttttatct tagtttatac tatgttcgat ataaaacttt gtttcctaat gtcaaaaaaa
    18721 catttcttat aatacttctt ttatcactct taaagcattt ttatagtata tcttttctat
    18781 atcatcttca ctaaaccctt ctttttttag aggttcatac agtttaccca tctgagatat
    18841 atcttcaatc tcaacttttg aatctattcc atcaaaatct gaccctagag atacaacatc
    18901 tattcctcca acatttacaa tatgttttat gtgtctaacc atatcttcaa gtttactttc
    18961 acttttatca cttaaaaata agtggaagaa gtttatacct gtaactccac ctttatttgc
    19021 taatacttta atcatatcat cagttaaatt tctagaatga ttcatcattg ctcttgaatt
    19081 agaatgtgta gcaattattg gtttagaaga aatctttgca atttcataaa atccaccatc
    19141 tgaaatatga gaaacatcaa ccaacattcc caattcattc atcttatgta ccacttctct
    19201 tccaaaatca gtaagccctt tttctctata ttctacttta ttatgaggaa agctcaattc
    19261 attcacatga ttccaactta ttgtcatcat tctaacccct aagtcgtaaa atttctttaa
    19321 attttctaat ttaccttcaa gtactgcacc ttcctcaata gataaaagag ctgtaagttt
    19381 accttctgat tggtttttca taatttcttc atagttagta gctaaagcta tttcatcact
    19441 attttttttc atttcctcat gaaatttatt tgccatactc atgcaataat caaaaggatg
    19501 ttttacttct tcagtatcaa caaaaagagc aaatgtttga gctaatgaat cccccttttt
    19561 caatctgtct atatcaacag aatatttatt agacttaagt tcacttgttt ctacattttc
    19621 cattaatttt gctatagtat cacaatgtaa atcaataaat ttcatattta taccccctgt
    19681 atattttata tttatatcta aaaatagaaa tagttgtcct tatagacaac tattgacaac
    19741 tattttttaa tattcttttt aatattactt aatcttattc ttcttttaag ttaggtatta
    19801 cttttttaac ttcttcaatt tgatgccatg ttttatctat attattttca ctaatatatt
    19861 tttttaaatc agttaaagat gtaaaatctt taactgataa attgtcatca tcatctgcaa
    19921 atcttaaaat aaacatgaga ctcccttact ttccacaaca tttcttatat ttttttccgc
    19981 ttccacatgg gcatgaatca tttcttccta tttttgcttc tttaactatt gttttagact
    20041 tcttataagc ctttattatt tcttttcttt ggtctgctgg tagtatatct tcccaacctt
    20101 gtaaattata tagccaatga gcctcaacag ttaccatatt gttatataat ttttcccaat
    20161 ctatttttat agaaacttct ttatcagctt ctacttcttc taaaactata tcttctttta
    20221 agctttcact tattccatct atgaatccca tgaaaaattc attagtagtt ccaaattttt
    20281 cagccaattc acttacttta ccttcaacaa catccatttt attatttaat atttcattgt
    20341 atattccagc ttcaactttc aaatattctt cccagaattt tacttctgct tcttgacttt
    20401 catgattatc acttaaattt ctccattcag tatataaact catacttttt cctcctaagt
    20461 atacctatat ttttatagtt tttatagtat aaaatctttt agttagattg gaagtttctt
    20521 ctttttgatt ataccatatt ctatttaaag ttaactctta tattatatca taattaaaca
    20581 attcttttaa tacttttata gcatactctt ggtttataaa ataagggctt gtcgcaattg
    20641 ttattacttt ggctttttta attaagtttt ttatcttgta aaatttgagt tcaaatggta
    20701 tatagtccat atcacttgaa aatatatcca aatctatgtc aagaacaaac tctgattcta
    20761 cttctaaatc aaatccatat agactatcta ttattattaa ttctgaaaat atgttatatt
    20821 ttaaagcagg ttttataaaa cttcccacat ttaaaacttc atttgtatat ctaaatacat
    20881 cttttagatt atttacatct acatcatagt tctcaggttc tctagtatcc ttatgttggt
    20941 ctacatgcac taacttgcat ccttttgtaa actcaccctt taacaaagat tttgcccaaa
    21001 aataaaatgc atggttatga ttgtcaaaca catatatctt tttatttccc aggacatatt
    21061 cgaccatatt ctccaatcct tttgctttta tttccttatt ttcatctact tcattaaata
    21121 ctacctcatt gcctaacctt acatcattta atgtaccatt tattagtttt ggaacgtata
    21181 tgctcttatt ttctctttta tcatatgaaa atacattatt tcctactggt ttttctatat
    21241 aaaatccact atattcataa ttttccataa aactttattt cctccattgt tatataaata
    21301 tataagttat aattttaaaa attaaaatgc acttattgta aatcatatca tctattatag
    21361 aaaacatctt atataactaa atataatatc ttctctcaat atgtattata caataagtgc
    21421 aaaatatatt tatataaaat atactacatt cctataacaa aaccatcagc ttcaattgga
    21481 aggtaactaa ctcttctatt caactccacc tttccaaaaa atactaccca atatggtcta
    21541 aaaatacttt caattttaac tatttcaatc tctgggattc ctcccataaa cctatgagtt
    21601 aatctcatag ccattttatt tgcctttgct tttactcttc gttcgtcctt atcactatac
    21661 tgcacaatat tacaatccac attttttaca acttcatatg taggaaaatc ttgtactata
    21721 ctaccagaac ctgtagaacc attcactaaa atttttaatt catttatatc attagaatat
    21781 ctcctattct tgaaaatgga tttcgtttta tgaattttaa gtgtgataat tttaaactct
    21841 atataatgaa gtctaacttc atgagcatat ttgttcttta atattagctt actaaataaa
    21901 gaattttttc cacttttagc tatctctata gcttcttctt ttgtaattct tataggtaac
    21961 gctcttatat ccataaaatc attctctttc ttattttttt cttgtcattt ttaaaactct
    22021 atatatataa tcaaaaaata ttacaattac aactgacata actccatatg gaattatttt
    22081 tccaactata atatcatttc tagtcaatat accataaaat ataaatcctg ttattaaaaa
    22141 gtacaataat actagaaaac cgtctattcc aagccatttt atttctaaat ctttttttac
    22201 cttagacact aatttagaca tagcagtttc tccttttata gattattttg tgtatttaaa
    22261 ttttcaccta ttctattacc aatcatccat gcaataaatg aaaaaggtat agctatcaca
    22321 agaggctcta actcaaagct ctgaactgga aatattttaa aataaccaaa agtcactcca
    22381 ccaacaatca ttcctaaaat accacccata gatgttcttc ttccatttcc aagaagccca
    22441 cctactatag gtacaaatgc cgaacccatc caaatacttc atacaaatat acatgcatca
    22501 tatacatttt caaatctaaa tgctacgcta agccccataa ctgctgctaa acatacacct
    22561 attctagtaa actttactaa ttgtttctga gataactctt tttttgtaat tcttccatat
    22621 atatcatttg ctaatgtagc cccagctgct agataatagc tatccaatgt tgatattata
    22681 gaacctaaaa ttcctattat aaatattact cttattcctt gtggtaatgt atctaaaact
    22741 aaagttatat atccagcagc tggagccatt tctggataag tagctcttac tataagtcct
    22801 gtcatagtta acaccatgtc aaaacatatc catagtgaaa aacaagttaa taaggctctt
    22861 cttccaacct taggagaatc tgctgctgaa aatctttgat aaaaagttgg gtctgaataa
    22921 gctccaaaag aatatacaat taacatcaat gctttcatag ctgacatacc ttttgtaggg
    22981 tgcataagct gtggtgcatc tttgagtgct tctgtaagac cttgaaaccc tcctatttgt
    23041 tcaaattgac ctggcataac catagttaaa ccaaatacca tacaaaaaaa caataccata
    23101 tctgtaatgg caactcccat aagcccgcca agtactgtaa gtacaacgac tactcctacc
    23161 attattaatc ctattaattc tttatctatt ttccatgcta cgttacctat agttcccata
    23221 gcagttattt ctccaagttg tgcacaatat ataaacatca aaattgcccc aattatagca
    23281 acatgcttgc cataaaaact ttccagtaaa tcaggaactg ttatattggt tcttatatga
    23341 atccttggac caacccacaa tgctaaaggc attctcccaa tatgtgcagc tattgaccaa
    23401 actgcccatg cagatattcc atatatagct gcatactcta cactacctac tgtaccaact
    23461 cctccatacc atgatgcaac caaagttcct actattaagg accatggtaa acttcgattt
    23521 cctaggtaat aactttccat atcttttaca tttctagaaa aataataacc tattcctaat
    23581 aatataaatg caaatataac tataatccca ttttcccaaa atgttaacat atctaatctc
    23641 cacattctta tattttaatt tttaacttta atatctaaat atgaacaact ctacccattt
    23701 ccataaaaat aggtagagtt atataataat tataagtatt taagtaatct tgttattaag
    23761 caattttatt gtttaaacct ttattttctc ttttatttat tttatttaaa gcataagtag
    23821 caaatatccc tataactgca catactacag ataaaataaa tatatatgta tatcctgttt
    23881 gtccatattt atctaaccaa ttacctgcaa tagtgtaata aaatgcatct ggagaaaatc
    23941 ctataaatga agcaaatcca actactgctc ctgtagtttc taaagatata ttagactctg
    24001 aaacagatgc aaagtatata cctctaaatc caaataaaat tacacttagt attatcatgt
    24061 ttattacagc tatccatata aatcctgtac tcttaggaat aaccaagtaa gcagctgtac
    24121 ttactgtcat taatataaat cctacaaaca ttactcttat agatgaacca actttatctg
    24181 ttataacacc agcaactgga gatgctccca tttttataac ataagttctt attattgata
    24241 aagcagaaac tagagtcatt ggcattacat aaacttctga taagtatgga cttaaataag
    24301 ttaaacttga gaatactaaa taagttgaaa atattataag accaattaac caagcctttg
    24361 gcattgtaac tacttttaac aatgacttaa tacttaatcc ttcatctgat gctccactat
    24421 cttttttctc ttccactaag aaaaatagaa gtataccaca tattatagat gctgcagaat
    24481 ataaccatac tacatatttg aatcctactg ttatttcagc aaatttacca aagaagtaaa
    24541 gacctataaa agaaattaat gtacctgata cacctgataa cccttcataa aaaccaaata
    24601 ttcttccttg ctctgactca cttccttgca ttctaacaac cttaacagag gctgagaaga
    24661 aggttaatat tgtcgtaaat ccccataata caaatattaa aagtatcata ctatatgatg
    24721 ggaatgtaga gaaataaaat ccaagtattc ctgaagcaat taaagaaaaa gttattaatt
    24781 ttctaactga gaatctatct gcaaaccatc ctcctataaa ataagatacc attcctacta
    24841 atccatataa actcaataag ttccctattt gtgtatgact taatgctaaa gcttgttgca
    24901 ttgggtcata aaatgatgat ttcatatatg gtaaactata catagctgtt gttccaaatg
    24961 ccaatatgaa tattatcaaa aatcttttaa cattattttt gctttccatt gattctctct
    25021 ccttaaaatt ttattgttta taatctcatt catataattt tattttaatt tttctattgc
    25081 ttgtttaaaa tcatttatta tatcatttga agattctaat cctacagcta ttcttattag
    25141 attttcccca ttcatttgaa ctaatgtatg ctcttcccct aagcttgttg ctattgttgc
    25201 aagctctaaa gaattcatta atcttctact agcttggtct cttgtcaatc cattgatacc
    25261 atccttaagt ttaaatgaaa ctacacctcc acctaaacca ttcatttgag ttttacaaag
    25321 ctcatattga ggatgagatt ttaatgctgg atactttacc aattccacat tctcttcttc
    25381 ctctaaaaat tctgctactg ctaatccatt ttcacaatgc tttttcatcc tcatatctaa
    25441 cgttttcata ccccttaaaa tcatccatgc actcattggt ggtaaagatg ctcctgtaaa
    25501 acaacataaa cttggccatc taactaaatc tattaattct tttgaacctg ctactgctcc
    25561 ccctaaagta tccccatgtc catttataaa cttagtaaga ctatgtatta ctaaatctgc
    25621 acctaaatct attggtcttt gtacaattgg tgtcccaaaa gtactatcaa ctatagctaa
    25681 gcttttattt tcatgagcaa tttctgctat tgtttttata tctactaagt ctaatgatgg
    25741 atttaaagga ctttctatat aaactatttt agtatttggt ctcattgctt cttttacatt
    25801 ttgtggattt actgcatcta caaatgtaac ttcaactcca aatttagata acaactcaga
    25861 catgaataat ttagtgtgtg aaaatattcc atctgaacta actatatggt caccactttt
    25921 taccaatgct aatatagtag aggttattgc ccccatccct gatgtactag atatggcact
    25981 ttctgcattt tcaagcatag ccaacttagc ttctaactct gtaagagtag gattacactc
    26041 tcttgtatac acatatcccc atgtcataca caactcttca aaatgctcta ctgtctttgc
    26101 agcaaatgtt gctgtttgac atattggtgt agccaatgca cctgttgttg ggtctgcact
    26161 atgtccccaa tgtattattt ttgtctctaa atcttgatta atattgttac tcattttgat
    26221 ttcctcccat tttgacttat ttatacaacg ttttccctgc caaatttcat caatttagct
    26281 tagtcttaat acattttttc aagtatactt ttttatttgt tataatttct aaaaatatga
    26341 cacttatttt gtttttttac agttctaatt ataaactcta tagtaactat agagtcaata
    26401 tgtttttgat atttttttta caatgtttaa aatagtcagt acaaaaatag gtatatctta
    26461 gaataactaa agtcatccta agatatacct atttttatat cacaatataa ataattacat
    26521 ataagcatta gtttttttat cataaataga tttttcaaga tttatcatct tttcataaat
    26581 atattatagt taaatatttt aatattacct aacatctaca ataaaatata ctcttaaaat
    26641 aaaattatat aattaaaaat actctataaa agctaaaaag tttttctatt cgctacatct
    26701 aaaaataatt agatagaggt ataaatgtat ttagtttatt tatacctcta gtcttttata
    26761 aattatatct ctactaaatt gattttttag cacgaataag tgtgctcttg gagataccag
    26821 tcatcttcac aacctctcta tatggcttag aatttaataa ccccaatgca tattgtatct
    26881 gttgattgct gtatttcttt ggcctaccat ctttaaagtc ctttttcttt ttagcaaatt
    26941 cttttcctaa ttgagtacgt tctattatca tatttctctc aagttctgca actgcaagta
    27001 atgtagtcaa aaagaattta cccattgaag tattttctaa aaggcctata tttaaaatat
    27061 gaacacttac ttctttagca aataaacctt ctattagttg tattccttct gatgtacttc
    27121 tagctagtct atccagctta gttgcaacaa gtaagtctcc cttttttaga gatgatataa
    27181 ttttttcaaa tttaggcctg tttgtagtag tgcctgtata agtttccttt attattttag
    27241 catcattata tttattcaaa atttcttgct gttgctgata caaactgttg ttgtctgttt
    27301 gattttttgt acttacacgg caatatccat atataataat catcacttcc taacataaag
    27361 attttgtaca gaagttaccc agaagcttgt ttttacttat ctttatccat tttactaaaa
    27421 cgatttttgg gtgtcaatac tgttaacttt tgacaccata ataattcctt tataatttgt
    27481 ttgactatgt caaaaattac caatataatg aaattaatga gtttatacaa attggaggaa
    27541 ataatatgaa tagtctgtta aaagaagcaa tagatgaatt aaaaaacatc cattgcgggg
    27601 aagttttttt attaaaagaa ttgttcaaag gctatgaatg gaacaggttg gagatgaata
    27661 tcagattgaa attaggaaaa ctttttttgt atgaagtggt aactaatccc aatttaaatg
    27721 tagaggtcat caataaaaat tctgcttatc agcaaatgta taaaaaaaaa tagtgactta
    27781 aatgtatata atagtataat cttcttattg attcaaaagt taaaactttt aatacaaaac
    27841 aatcattcta ttttaacatt tatcaatatg tctaaattta tataattaac acatacgaat
    27901 agtattgttt aaatcacatt atttaagata gataaatctt aaccctactc cagcataaat
    27961 actaaaatag ggcttagaat tatctatatt tttatcccat aaaatgctta gctcttcctc
    28021 ttgattgaac atatatatct ggggaaatac aatagaagcg taaatttata aaatttccaa
    28081 agtcagaaaa cttcagttca aacttaggta taccaaaact ttcaggtgct atttcgtcaa
    28141 caccaaaata gattataatt ccattatctg taagataaaa tgcttggtct tcaggtataa
    28201 ctaactcaac attagaataa tacatttctc tattttcatt tatttgagat ttaatgtaat
    28261 cagtaattat attaatataa tcaactccat ctctaaaaat gtcattaagt aaaagttctt
    28321 ttccagttgt caagtctata ttgtaattat tcaatgtatt gtatttaggc ccatagttat
    28381 tagcaaaccc cataaggttt aatatcatac tcagtacata atttttatta aaagtaactg
    28441 cgtaactggt agatacccaa tagctttgtt ttggaagtga attttgagaa gctgcgttat
    28501 tatattctcg ttcttcttcc tgtaagccat ttttaaaatt atttacagtc attcttacag
    28561 cattgtttat gttatttaaa ataactgggt ttacatacgt ggattgattt ctaatattgt
    28621 atataacagg atactttaca tcatatgaaa aatacttact tctttcaaaa tcttcattaa
    28681 attgtacata taatatatta tatgtacagg tatttgtaga ttgataattt tccattaaaa
    28741 tcctccccct tttataaaaa aatcagatac aatataatat gcttaaaaca gtaataatgt
    28801 taatggtaaa tgtaaagaat agaaagtatc taaaacagca aaaaatgact aattttgtgg
    28861 tataatacat aggattgaga gttattttat aggaggaaaa atatgttatt attagcagat
    28921 aaatggaaag attatgaact tatagatatg ggaaatggag aaaaattaga acgttgggga
    28981 agttatgtac ttagaagacc agacccacag gttgtatggc caatggaatc tgaatgggct
    29041 ttatggaaaa accctcatgg acattatcac agaagcaata aaggtggagg tcaatgggaa
    29101 cacaaaaaaa gatatccaga acaatggact ataaattata aaaacttaaa gtttcacatt
    29161 aaaccaactg gctttaaaca tactggatta ttcccagagc aagcagctaa ctgggattgg
    29221 atgatagaga aaataaagaa agctaacaga cctataaaag tgttgaatct ttttgcatat
    29281 acaggtggag ctactgtagc atgtgcatct gctggtgcag aagtatgtca tgttgatgct
    29341 gcaaaaggta tggttaattg ggcaaaagaa aatatagaat tatcaggtct taaaaatcaa
    29401 acagtaagat ttatagtgga tgatgttgta aagtttgttg aaagagaaat aagaagaggt
    29461 aaaaaatatg atgcaataat aatggaccct ccttcttatg gaagaggtcc taaaggagaa
    29521 gtatggcaaa tagaagaaaa gttatacagt tttgtagatt tatgtatggg agttttatca
    29581 gataaccctt tatttttctt aattaattct tatacaacag gattttctcc aatagttctt
    29641 gaaaatgtat tgtctacaac aataggtaaa aagacaaata caggagatat atacggtggg
    29701 gaattaggaa taccagcttc cagagatggt aagatacttc cttgtggtat atttggaaga
    29761 tgggagaaaa aataatgata aaagtcatat atgaagataa ccatttatta gttgtagaaa
    29821 aaccagttaa catattgtct caaggagata atactaatga taaagatatg gtaaatcttt
    29881 taaaagacta tattaaaaaa aagtataaca aacctggaaa tgtatatatt ggtttaatac
    29941 atagattgga tagaccagta ggtggagtta tggtttttgc aaaaacatca aaagcagcat
    30001 ctagattgtc tgagcaggta agaagtaaga ctttcaaaaa aacttatcta gctgttgttc
    30061 atggtaagat aaaatctaac tcagatatac taaaagattt tttatataaa aataaaaaaa
    30121 ctaatatggt aactgtagtg aaaaaaagcc ataaagatgc caaagaagct gaacttaact
    30181 ataatgtatt agattttaaa gaaaatctaa gtttagttca agttaattta aagactggtc
    30241 gttctcatca aataagagtt caatttgcaa gcagaaaaca tccattatac ggagaccaaa
    30301 gatatggcaa agaaataaat aagattggac aacaaatatc tctttggtcc aataagatag
    30361 aaatttgcca tcctactaca aaagaaaaaa tggaatttac atgtcatcca cctgacaaat
    30421 atccatggaa tatattttaa atctcaaatg aggtgattat atgagtaaaa aattttcaat
    30481 aggagaaatg tctaaacttc ataaaataag catacagaca ctaaggtact atgaccaaat
    30541 agatttattt aaacctgttc atgttgacca agaaagcaat tacagatatt actcaattga
    30601 ccaattttca caattagata ttataaaata tttaaaatat ttaggtatgc ctttaaaaga
    30661 aattaagtca aagcttgatg aaaataatga agaaattcta aaattactaa caaataaaat
    30721 taatatagtg gatgaaaaaa ttaaagaatt agagcttata aagaaggtac taagcacaaa
    30781 gaaagaaact atggaaaaag gtatgaaagt agatgaagtt ggtaaaatag tgagaaaaca
    30841 tattccacga agaagtatct tatctataga gtatgaaaaa ggattaagta gtgaagaaac
    30901 tatggaactt tccagaagac aaatagcaaa tatattagaa gataatatat ctgtgttcta
    30961 tggtggagta agtggacttg tatctataga taaattttta aaagaagata aagttattta
    31021 tgacaatagt tttgttactg ttgaaagaga cttatttgat ataaaatcac aaaaacatat
    31081 aacagaaatt tcatctggtg attttatttg tatgacatat aaagggcctt acaaagaaaa
    31141 ttataccgct tttaagaaac tggttgatta tgttgaatat aaaagaatac ctattgaaaa
    31201 ttatatatat gaaataccta taatagaccc tctgtctaca aacaacgaag aggaacttct
    31261 aacagaaatt caaatacaag ttaaaaagag agaaattgac ttaagatgtc tagtgtagta
    31321 atctagttta cttacacatc ttaatatagt tgtctattat caacatatga tttttctaga
    31381 tagtgattcc taacagtgag attttcaaaa aataggagga aatatggcta atgctaattc
    31441 cctcctattt ttaattaagc catatcattt taattaagcc atgtcatcac gaagtgcatc
    31501 aataatattt tcttttttta tcttattaat agtatacaac attgtaataa atactataaa
    31561 taaaacacta aatatactaa ttccaatact aaaccatgga atcacaaaat caatatcatc
    31621 tgtaaccatt actttatgaa ttagccatga agaaattact gctataggaa gtccaaagaa
    31681 caatgctctc atgccataaa aggcacactc aaaattcatc atcttttgga aatcatgctc
    31741 agacattcct acagagcgaa gcatggcaag ttctctacga cgtaacttaa tattagttga
    31801 aattgtatta aacacattgg caatcgaaat cagagaaatc ataattatga aagtataagc
    31861 aaaaacattg gcaataaaaa tgtaattacg gttctcatcc atcattccat acatattgta
    31921 cagattgtac tcattcgtaa ctccctcact ctcaataatt tgctccattt ctgcaactga
    31981 ctgagtagga tttttcgatt gaaagcttaa actttcaatt cctgtagaaa caccaggaat
    32041 ctcaaactta tctttggctg aataaggagc caatactctg aagcttgctt tcttcacatc
    32101 tgaacttctg attcctggag gagaatcagg tggcactact ttgacaaatg aaatattcac
    32161 attttgtctc tcaggtatct ccaacttgcc attagtttca ggagtgaggc taacattcat
    32221 agaagaactg ttgaaaatat caataatatt attatccttt gttaccatag aaacagcaat
    32281 cagttttgca ttttgtccag tatattcttc tgctggtaat ccaagacttt taataatatt
    32341 cagataagtt ctatcatcaa gaaattgcat atctactggt aattcaagca tttcactcat
    32401 agaatgtgaa ccctcatagt tcaaataatc gtctgtaagc tcacttactt tggcaacaca
    32461 tgaataggtc ataagtttct gatacaaacc ttcgtaaaca ccatttgcag tttttagctt
    32521 atcgtaaagc ttcaacattt cgttgtcttt cataccttgt gtagaaaaaa taatatcctg
    32581 gtcagtaacc tctgacctct gatttgacat ctgttttagg tctgctacta aagcacttgt
    32641 agatataaat agaactatgc ttaaaacaag cgacaataca atacttcgat aacgtttctt
    32701 gtttctttta aagtttttca tagcaagaac tccctcaaga ccataaactc gctgtgttag
    32761 ttttgatgtc ttcacagatt tagattcaat ttttacctca tttgtctgac gaatacactc
    32821 catcactggt gtgttggcag cctttcttgc tggtatgtaa gcagaaatta agattgtaac
    32881 catacttacc actgctgcaa cgatgattgc agggatagac aatgttaatg tcaacggaac
    32941 gtttacatag agaatatttg caaagttttt agctatgaca gaaatcacaa gtccaatact
    33001 gactataccg attgcaacac caattggtat accaatcgca ccaatgcaaa ttccttcaaa
    33061 tagcaccgaa tttcgtaact gttttgctgt agctccaaca gatgaaagta ttccaaattg
    33121 atgtgttcgc tcgttcaatg acatgttaaa tgaattatat attagaaaaa ttgagcctag
    33181 catgattatg gcaagcacaa tgccaccaac cgagtacaaa aacatattga acaaattgtt
    33241 atcagaaaca cctatgaagc gcaacacatt gtcattgaaa atggaagcat ggtctttagt
    33301 gctacttatg taagagcgaa tctgatgtgg atttttcaat gtgacaaaaa ggctaagact
    33361 gtcagtcttg acttgtttat cttctttggt tatcaaggta tatcctggtg cagaagattc
    33421 ttcaaatacg ggctttctac agatacccac aactgtataa gttttcttat cctgtggcac
    33481 aaaaacttca ctcccagcag tgtatgggtc actctgacca agcttttcat tttcactcat
    33541 tcgtttccct acatccaaag aaatagtatc acctactgca aatttgacac caccatctgt
    33601 cgcaacttta cttgaaacaa gaatctctct actattttca ggtaatcttc cagaaattaa
    33661 agttataggc aaagtatcaa aagcttcttt gctaaatcca gcaataaaaa gatatggttt
    33721 atctggattt ctgttacctt caagtgttgc atagccaata ttttcaaatg ttgcagtatt
    33781 tgcaacttca tcattgactc tttgttcctg tacaaaagaa gaatccacat ccaaaaactg
    33841 gacatgccaa tcaccatatt ttataatagc tccatttgtc atatagttca tcaaagaaac
    33901 tccaaaggta gtaactgctg taatcatagc agctgacaga acaactccta taattgttac
    33961 aagagttcga gtgcgattct ttttcaatcc ttgcagagca actttgttaa aaatgttcat
    34021 taacgattca ccttcctctc atcacgcact acttttccat ctgaaataca gataattcta
    34081 tctgcctgta aagcaatgtt ttcatcatgt gtgacaataa tgagagtttg gtgatattta
    34141 ctatggctct ctttcagtaa ctggataatc tcttgtccat ttttactgtc taagctacct
    34201 gtaggctcat cagcaagcat aactgctggt gcattcatca atgcacgacc aatggcaaca
    34261 cgttgctgtt ggccaccaga aagttgattt ggtaaatgtg tttttcgttc ttttaaacca
    34321 agcaattcta acaagtcatt taaccgttct tgattgacct tacgcttatc cataagtata
    34381 ggcaatgtga tattttctac tacatttaat gtgggtatca gattgtgaaa ctgataaatc
    34441 aatccaactt gtcgcctacg aaaaatagca agtttttcat tgctttggcc atatacatct
    34501 tgcccctcca gatatatctt tccacttgtt ggtacgtcta ctcctgcaat ggaatgtagt
    34561 agtgtagatt taccagaacc agaagaacca atgattgcag taaactctcc tttttcaata
    34621 gtaagtgaga catggtcaag tgcagtaacc ttattttcat tcttaccata aaccttacat
    34681 aaatcttcaa tctttaaaaa cgccattata taagtctcct ttcatatttt ataaatatat
    34741 cataggactt tcaacttaca tttaagtgac atttaaatga gagattcgtc actttggaaa
    34801 acgaataaca aatattgcac caccttgtgg gtgattttgt gcagtaattg ttcctccttg
    34861 tctcataata atcgtcttac aaagtgccaa tcctattcca taaccggctg tgcttgaatt
    34921 tttcccacga taaaatctgt taaatagaca tggtaaatct tcttttttaa agcctgctcc
    34981 gttgtcatga atagtaagtt gagtaaacaa aaagttgtcc tcacaaatga tatcaatttt
    35041 tccattatca cccacactct ccatgcagtt tttaaatata ttttgaattg cttctgaaag
    35101 ccaatttaaa tcacccaaaa tccttactcc ttttggtata tcaatatgta aagtgatatt
    35161 gtgcagttcc atgggaatca aaagttgatg aagtgcagaa ctaatcaaat tattcacatc
    35221 aatttgttct ttttgaaaga ctacaatacc tgcatctaaa cgtgataatt ttaaaaggga
    35281 agtaatcagc cagtccattc gtataagtaa ttcctctgtt tctcgtatca actcttttct
    35341 ctcatcttca tcagaagtat tctctaacag tgacaaaata agagttactg atgtaagagg
    35401 agtgcgaagc tggtgggcta tgtcagctag tgagtcagca aggtgttctt tttctttttt
    35461 caatgcgtaa ttttgctctc taatacgtaa cgtcatttta gttatttcac tttgcagaat
    35521 agaaagttca ccctcatctg attcagcaat atacagatgg ttagcattat gaagcacaag
    35581 attaatttgt tctgaaatct gtgcaatact tttgtatcga tttttagtaa acacaaaaaa
    35641 tgctattcca aaggtagtgg cagaaacaat agaaagtatt ccagtcataa tactgattgc
    35701 aaatcccagg gctacagtga gaatagtaat taataagaat aaaatagcaa attttcgaaa
    35761 ctctctattt cgaaacataa cttcccccca atctataccc tgtcccacga acagtcaaaa
    35821 ttatttgtgg acttgaaggg tcatcttcta tcttctctcg caagcgtttg atgtacacag
    35881 tcaatgtgtt gtcattaaca aactcacctg ccgcatccca caattcatca agtaatctta
    35941 ccctcgtaat aatactttta ggattgctaa taaacaccaa taacaaacgg tattctaaag
    36001 ctgaaagaaa aacctcattg ccattctttt tcacaacacc ggttgccgta tctacataaa
    36061 gcccacaaat ttcaaaagtt gacccaaaac gcccactttt tcgtagtgct gttctaattc
    36121 gcgcaatcag ttcacgtgga cgaaaaggtt tagtaatata atcatctgca cccatattca
    36181 gtccagtaac aacacttgat tcatcaccgg aagctgtcaa aaagataaca ggaatgtctt
    36241 gcttttcttt gatttctgtg caaatcgtaa acccatttcc atcaggtaaa gaaatatcaa
    36301 taagtgccaa atcaaattta ttcttagcaa tctctacaag tgcttcactt cttgtggata
    36361 catgggttac tataaatcct tctgaattaa gcaaaagaat aagatttttg gcaattgctt
    36421 tatcatcctc aactaaaaat atccgtttca tttactttca tcctccttta ttttattagt
    36481 tttatatatt ccagcagagc aatagtacac tactatttta cttatcatta cacaattttc
    36541 agtagtttct tttgttctta tataatccat gcataatacc tccattgatt tattatattt
    36601 cttataacat aaatacacaa gataagacaa aaaacataat gtaagtgcaa gttttttaac
    36661 tcactaactt ataaacatac actcctacta acactacttt cttgatgaca catcttacaa
    36721 gcttatattc atacatcaat gaaaaattaa atttatccat ttattaaaat aaaaggcaat
    36781 agcttctata gctaatgccc tttatcttaa aatgatttaa atactataca atttttataa
    36841 atggctataa ataaactcgt atagaatata aaatttatct acagtctttt gttatacaaa
    36901 atttataacc tcttatagaa caaatttacc actttgcaaa cttatcactt ttactcagct
    36961 atattataaa cttttaattt atctttgttt aaatgtttct tcttaacttc tactaaagct
    37021 tttagcgtat ccaaagatgt cattatttca gtagaaaatt caatggctgt tcttctcatc
    37081 ttaaatccat ctcttgtaga gtcatttcct ttagttggtg tatttattac tatatcaact
    37141 tgtttatttc ttatagcatc taatatgttt ggtctaggct cttcaattct atttactata
    37201 tcagcctcta ttccattttc tcttaaactc ttcgctgttc cttctgtagc aacaaaagta
    37261 tatcctaatt ctttcatatc ttttgctatc tctataaact catctttatc atgattattt
    37321 acagtagcaa gtactactcc tctttcatca gacatatgtc tacctgctgc caagaaccct
    37381 ttatataagg cttcttctaa attttctcct acacctagaa cttctcctgt agacttcatt
    37441 tcaggtccta gacttacttc aactttagat aatttagaca ttgagaatac tggtactttt
    37501 actgatacta attttggctc tttgtaaact cctgtactat atcctaaatc ctttaattta
    37561 gctcctaaca tacacttagt agccaagtca actattggta ctccacttac tttacttata
    37621 tatggaactg ttctactagc tctagggtta acctcaatta tatataattc attttgaaat
    37681 tctataaatt gtatattaac cataccaagc acattaaggt ctaaagctat ttttttagtg
    37741 tattctacta ttttttcttt tatttcatca gatatatttt gacttggata cattgttgta
    37801 ctatctccag agtgaactcc agctctttct aaatgctcca ttataccagg tatcaatata
    37861 tcttctttat cacatatagc atctacttct atttctctac cagttagata tttatctata
    37921 agtactggat ttttatggtc tctatcaaat gcatcatcca aatactgaat tagtttttct
    37981 tcgttataag ttatttccat accttgacca ccaagtacat aagatggtct aactaatact
    38041 ggataaccta actccttagc aatttcaaca ccttcattag ttgtccatac accttttcct
    38101 tttggtctat ttatatctag tctctctagt aaatcatcaa atttttctct atcttctgct
    38161 gcatctatat ctctaaagtc tgttcccagt ataggtatat ttttttcatg taaaaactta
    38221 gccaatttta tagctgtttg tccaccaaac tgtagtatta caccttctgg tttttctttt
    38281 tctattatat ttaaaacttc ttcttctgtt aatggttcaa aatacaactt atcagatgta
    38341 tcaaaatccg tacttacagt ttcagggtta ttgtttacaa ttatagtttc tattcccatt
    38401 tttcttaaag attttacaca gtgaactgaa caatagtcaa attctattcc ttgacctatt
    38461 cttattggac cagaacccaa tactacaact tttctcttat cactaactac tacctcatca
    38521 tattgttcat aagttgaata gtaataaggt gatagagctt caaattctcc tccacatgta
    38581 tcaaccattt tataaactgg ttgtatatta tataaacttc tcaattcata taatttttct
    38641 gggcttatct tcattaagtc agatatacct ttatcagaaa atcccttttt ctttaattca
    38701 tgtaaatatt ctttatctaa gtcttctatt tttagaactt ttaacttttc ttcttgttct
    38761 actatccatt taaatttatt tataaaccac ttatcaacac cagttatttc tgatatcatt
    38821 tctactttat agcctcttct tatcatttca gctaagtcaa atagtctctc atcatcaggt
    38881 actactactc tagcctttaa ttcttctaat gttctacctt ctgatggagc atgaaccaat
    38941 gaatattttc ctgtttcaag tgatcttata cctttaagaa tagcagcctc aaagttactt
    39001 cctatactca tgatttcacc agttgccatc atcttagttc ctaattttct attggcatgt
    39061 ctaaatttat caaatggcca ctttggtatt tttactacca cataatctaa tgttggctca
    39121 aaacaagcat aagttttttt agttactgca ttttctatct catccaaagt ataacctaaa
    39181 gctatcttag ctgctacttt tgctattggg tatcctgtag cttttgatgc tagggctgat
    39241 gacctactaa ctcttggatt tatttctatt atcgcatatt ctaaactatg tgggtttaaa
    39301 gctatttgaa cgttacatcc accttgaacc tctattgcat ttattatgtc tattgatgcc
    39361 tttctaagta gttgatactc ttcatcactt agagtttgac ttggagcaac aactatactg
    39421 tctcccgtat gaactccaac tgggtctaca ttttccatat tacatacagt tatacagttt
    39481 ccatttccat ccctcattac ttcgtattct atttctttcc aaccttttat actcttttca
    39541 agaagaactt gtccaacagg acttagctgt agtccatgtg aaagtatttc tcttaattct
    39601 tcctcagtat cagctatacc tccaccagtt ccacctagcg tatatgctgg tcttacaact
    39661 actggatatc ctatcttatt ggcaaactca agacctgctt ctaaatttgt aactatatca
    39721 ctaactatta ctggttgatt tatttctttc attacttctc taaataaatc tctatcttcg
    39781 ccctttttta tagattctat agatgttcct ataactttta cattatattt atctaatata
    39841 ccagcatcat gtaattctac agctaagttt aatcctgtct gaccacccat acctgcaagt
    39901 aaactgtctg gtctttcttt ttctattatt ttttctataa attctattgt taatggttct
    39961 atatatattt tatctgcaat ttctttatca gtcattatag tagctgggtt actatttact
    40021 aatacaactt caattccctc ttcttttaat gcttggcagg cctgtgttcc tgaatagtca
    40081 aactctgcag cttgacctat tattattggc cctgatccta atactaaagt tttctttata
    40141 ctatctaatt taggcataac tctttctcct tatctaaaca attttcacag ttcttatctt
    40201 catcatctat acaatcaact ttaattactt tatttcatct taaattcaaa tttactcatc
    40261 aatacatcta tgttaattaa accaaacttt ttgatttaag ttattcttgt tttttagtta
    40321 gctattctaa tgttatttat tttactaatt ctaaaaattt atcaaaaata tagtcattat
    40381 cttttggtcc tgggcaagct tcaggatggt attgaacact gtatacatct agcttctcat
    40441 gtctcattcc ttcaacagta ttatcattta aatttatatg tgtcactttc atttgttcag
    40501 gaacttctga aacataataa ccatggtttt gagaagttat aaatactttt ccctcttcta
    40561 aatctttaac tggatgattt ccacctctat gtccaaattt aagtttagca gtttttcctc
    40621 caagagctaa agctaaaagt tgatgcccta agcatattcc aactataggt tttttaccaa
    40681 taagagcttt tatattttct ataacatctt ctaggtcttc tgggtctcca ggtccatttg
    40741 acaagaatat taagtcaggg tttatactca gtacatcttc tggagatgtt gtagctggaa
    40801 atattgttat atcacatcca cgagctataa atgatcttaa tatatttctt tttactccaa
    40861 aatccataac agccactttt ggaccatttc cttttatatg ctctacttct tttcttgtaa
    40921 cagttctaac tgcttctgta tttgaaaact tatccaattt atgtttaaca tcttctaatt
    40981 tactgtcttc taatgtaatt atacctttca ttgttccatt atttctaagt atcttagtca
    41041 aagctcttgt atctattcct tcaagtccga taactttatt ttgctttaaa tattggtcta
    41101 tatcaatttc acatctaaaa ttacttggag aatcactttt ctcccttact atgaatccct
    41161 ttacttgaac tttttcagac tctgctgatg ataaatttat accataattt cctacaagag
    41221 gataagtcat agtaactatt tgaccatagt aagaagggtc tgttaaaact tcaccatatc
    41281 ctatcatcga agtattaaat actacttctc ctacactttc ttctaaatat ccaaatgcct
    41341 ttcctataaa aactgttcca tcttctaata ttaaccttgc tttcatacta cagtacctcc
    41401 tataattctc tttaggcaaa ctaaagaaca ttaactaaat ataagctttt aaatatctat
    41461 atctattttt tacttttaca ttctagtttt atacttattt acatttattt tttatctttt
    41521 tctattatac tacaaatttc ttaaaaaaaa gaagttatct caaaagtaaa aactcctaga
    41581 aaaaataacc tctttttttg atttaataat taaattctta aactatacaa tcagcttaat
    41641 ttctattcag ctatattgta aacttttaac tcatctttat ttaaatgttt cttcttaact
    41701 tctactaaag cttttagtgt atccaaagat gtcattattt cagtagaaaa ttcaatagct
    41761 gttcttctca tcttaaaacc atctcttgta gagtcatttc ctttagttgg tgtatttatt
    41821 actatatcaa cttgtttatt tcttatagca tctagtatat ttggtctagg ttcttctact
    41881 ctatttacta tatcagcctc tattccattt tctcttaaac tcttcgctgt tccttctgta
    41941 gcaacaaaag tatatcctaa ctctttcata tcttttgcta tctctataaa ctcatcttta
    42001 tcatgattat taacagtagc aagtactact cctctttcat cagacatatg tctacctgct
    42061 gccaagaatc ctttatataa ggcttcttct aggttttctc ctacacctag aacttctcct
    42121 gtagacttca tttcaggtcc tagacttact tcaactttag ataatttaga cattgagaat
    42181 actggcactt ttactgatac taattttggc tctttgtaaa cccctgtacc atatcctaaa
    42241 tcctttaatt tagctcctaa catacactta gtcgctaaat caactattgg tactccactt
    42301 actttactta tatatggaac tgttctacta gctctaggat taacctcaat tatatacaat
    42361 tcattttgaa attctataaa ctgtatatta accataccaa tcacattaag gtctaaagct
    42421 atttttttag tatattctac tatcttttct tttatttcat cagatatatt ttgact
//
LOCUS       DAEORZ010000016        42168 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEORZ010000016 DAEORZ010000000
VERSION     DAEORZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42168
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.016"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            467..2011
                     /locus_tag="KRH39_000784"
     CDS             467..2011
                     /locus_tag="KRH39_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG8510707.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTRIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
     gene            2187..2555
                     /locus_tag="KRH39_000785"
     CDS             2187..2555
                     /locus_tag="KRH39_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8510708.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            2557..3282
                     /locus_tag="KRH39_000786"
     CDS             2557..3282
                     /locus_tag="KRH39_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510709.1"
                     /translation="MDKNINNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            3276..4040
                     /locus_tag="KRH39_000787"
     CDS             3276..4040
                     /locus_tag="KRH39_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8510710.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            4262..5512
                     /locus_tag="KRH39_000788"
     CDS             4262..5512
                     /locus_tag="KRH39_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8510711.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYDVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAIIDLFIWIVIVVVYQVKPPIIVLPTSFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            5718..6602
                     /locus_tag="KRH39_000789"
     CDS             5718..6602
                     /locus_tag="KRH39_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG8510712.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            6964..7581
                     /locus_tag="KRH39_000790"
     CDS             6964..7581
                     /locus_tag="KRH39_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG8510713.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            7689..10220
                     /locus_tag="KRH39_000791"
     CDS             7689..10220
                     /locus_tag="KRH39_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG8510714.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNIKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            10654..11355
                     /locus_tag="KRH39_000792"
     CDS             10654..11355
                     /locus_tag="KRH39_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG8510715.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            11405..12478
                     /locus_tag="KRH39_000793"
     CDS             11405..12478
                     /locus_tag="KRH39_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8510716.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLISKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIV"
     gene            12748..13848
                     /gene="vanG"
                     /locus_tag="KRH39_000794"
     CDS             12748..13848
                     /gene="vanG"
                     /locus_tag="KRH39_000794"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG8510717.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPILHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            13845..14651
                     /locus_tag="KRH39_000795"
     CDS             13845..14651
                     /locus_tag="KRH39_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG8510718.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            14669..16807
                     /gene="vanT"
                     /locus_tag="KRH39_000796"
     CDS             14669..16807
                     /gene="vanT"
                     /locus_tag="KRH39_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG8510719.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDMCGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            17121..17741
                     /locus_tag="KRH39_000797"
     CDS             17121..17741
                     /locus_tag="KRH39_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG8510720.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(17907..18443)
                     /locus_tag="KRH39_000798"
     CDS             complement(17907..18443)
                     /locus_tag="KRH39_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG8510721.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(18556..19230)
                     /locus_tag="KRH39_000799"
     CDS             complement(18556..19230)
                     /locus_tag="KRH39_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG8510722.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            19433..21307
                     /gene="asnB"
                     /locus_tag="KRH39_000800"
     CDS             19433..21307
                     /gene="asnB"
                     /locus_tag="KRH39_000800"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG8510723.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(21399..22835)
                     /locus_tag="KRH39_000801"
     CDS             complement(21399..22835)
                     /locus_tag="KRH39_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG8510724.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKGSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVNRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            23164..23742
                     /locus_tag="KRH39_000802"
     CDS             23164..23742
                     /locus_tag="KRH39_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG8510725.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            23781..24359
                     /locus_tag="KRH39_000803"
     CDS             23781..24359
                     /locus_tag="KRH39_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG8510726.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            24445..25074
                     /locus_tag="KRH39_000804"
     CDS             24445..25074
                     /locus_tag="KRH39_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG8510727.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDSVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            25064..27823
                     /locus_tag="KRH39_000805"
     CDS             25064..27823
                     /locus_tag="KRH39_000805"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG8510728.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGRDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            27910..29472
                     /locus_tag="KRH39_000806"
     CDS             27910..29472
                     /locus_tag="KRH39_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBG8510729.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKSWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            29473..30609
                     /locus_tag="KRH39_000807"
     CDS             29473..30609
                     /locus_tag="KRH39_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG8510730.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            30673..31749
                     /locus_tag="KRH39_000808"
     CDS             30673..31749
                     /locus_tag="KRH39_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBG8510731.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFADALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            31727..32965
                     /locus_tag="KRH39_000809"
     CDS             31727..32965
                     /locus_tag="KRH39_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBG8510732.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENKYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            33019..34107
                     /locus_tag="KRH39_000810"
     CDS             33019..34107
                     /locus_tag="KRH39_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBG8510733.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            34135..34926
                     /locus_tag="KRH39_000811"
     CDS             34135..34926
                     /locus_tag="KRH39_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBG8510734.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(35041..35595)
                     /locus_tag="KRH39_000812"
     CDS             complement(35041..35595)
                     /locus_tag="KRH39_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG8510735.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            35791..36993
                     /locus_tag="KRH39_000813"
     CDS             35791..36993
                     /locus_tag="KRH39_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBG8510736.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            37050..38030
                     /locus_tag="KRH39_000814"
     CDS             37050..38030
                     /locus_tag="KRH39_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG8510737.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            38278..39231
                     /locus_tag="KRH39_000815"
     CDS             38278..39231
                     /locus_tag="KRH39_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG8510738.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            39221..40177
                     /locus_tag="KRH39_000816"
     CDS             39221..40177
                     /locus_tag="KRH39_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG8510739.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFVIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            40174..40929
                     /locus_tag="KRH39_000817"
     CDS             40174..40929
                     /locus_tag="KRH39_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8510740.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            41021..41986
                     /locus_tag="KRH39_000818"
     CDS             41021..41986
                     /locus_tag="KRH39_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8510741.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT        16405 a         4328 c         7257 g        14178 t
ORIGIN      
        1 aaaaaaatta atttaggaat tatttgattt attcatttat aaaaattaat tactgatttg
       61 tcgaaaccat tgtaataaga aaaaaatgaa aaaataaagt gtgaatatgt tgcaaatact
      121 gtattttaat aaaaagtagt catttcttgt aaaatggata gtttatatct actttaattt
      181 tgtaaaattt tctgctactc taattataga atgtagtata ttcattgtct taactattga
      241 tttatatatt tcatataggt ataatagaaa ataaattgga ttattttggc atataattat
      301 ttattaaaag gtgattattt ttgtttaaat tgtctgaaaa attttatttt tatattataa
      361 ttttaatatt ttcttttcaa aactatccat tttacaagaa atggaaactt ttgagatgta
      421 cttatttgct aataaaatag aggtgtttta atgggaggaa atttttatga ggaatttaca
      481 aaaaaaattg atgttttttt tatttatttg tataatttta caaattacat acacaacacc
      541 agtctattct ttatgcaaac tctataaaaa caactccata aataactttg agtctattgt
      601 gtctaataat cagcttatta gcaaacatgc aatacaaata atacttatta tggctataat
      661 atgtgtaata cttatattct atataatata tgataaatta aattttaaaa ttaaattgca
      721 aaggatagca tatacagata atcttacagg agcaaatact atagataaat ttgtaattga
      781 tgctaacaaa atactatgta aaaatactca agtaaagtat gctttattat atatagatat
      841 tgataaattt aaatatataa atgatttatt tggatatgaa gttgggaatg aaattttgcg
      901 taatttaaca aggattatta agagtaatat atttgaagag gaaatgtttg caagaatatc
      961 tgcggataat tttattataa ttatgaaata tatagaagaa gaagatataa caaagaggtt
     1021 aaaaactata tttgaagaat tagacttgtt taataataat caagaagaaa aatataagtt
     1081 agttttaagt tgtggaatct attttatact tccagaagat agagatatca actcaatcat
     1141 agatagggct aatatacctc ataaaatggc aaagggagga cataaaagtt catatgcatt
     1201 ttatgataat aagattcatg accaagagat aaaagaaaaa gagatggaaa atacaatgtt
     1261 ttcaagtcta gaaaataaag aatttattat atacttacaa cctaagatag aacttaatac
     1321 aggagagata caaggttcag aagcacttgt taggtggaaa cgtcctgata aagggctaat
     1381 acctccaaat gaatttatcc cattttttga aagaaacggt tttgttataa atttagattt
     1441 atatgtgctt gaagaagtat gtatatacct tagaaaatgg attgatgcag gaataaatcc
     1501 agttactgtt tcagttaatg tttcaaggat acatttatat tgcaataatt ttatagaaac
     1561 atacaaaaat ataattgata aatacaatat acctgcaaag tatatagaac ttgaattaac
     1621 ggagagtatc atatttgata attttgatat tttaattgat ataatgaata atttaaaaaa
     1681 aataggattt ttaatatcta tggatgattt tggttcaggg tactcatcat taaatatgtt
     1741 aaaagaaatt cctatggata tcttaaaact tgaccaaaaa tttattatgg aaacttataa
     1801 tagtaaaaga agcaaaataa ttgttactaa agtaatagaa atggcaaaag agttgggtat
     1861 gaaagtcata tcagaaggag ttgagacaga agaacagttt aaacttttaa aagaagtaaa
     1921 gtgtgatatg gcacaaggat atttgtttgg aaagccaatg ccaattgaag aatttgaaca
     1981 tttaattgta tctaacttgg taagagggta attaatctgt aatataaaga atttaataaa
     2041 ttgtgttaaa aatggactct aatgtagtgt tcattttttt tgtaaaattt tttatacttt
     2101 tactaaaaag taattgacat atactttgta ttattatatt attgtattaa caacttaata
     2161 caataatata ataaggaggc atgaacatgg aatgggaact tgataataac aaacctatat
     2221 atatacaatt agtagaacat ttaaagttaa aaataatttc aggggaaatt aaaataggat
     2281 ctaaattaga aacagtgaga gcactagcag aagatgcgga agttaatcca aatacaatgc
     2341 aaaaggctct aacagaactt gaaagacaag gtttagttta tagtcagaga actaaaggaa
     2401 gatttgtaac agatgataag gaaaaaataa aagctatgaa agaggaaata gctaatgtgg
     2461 agataaatac attaaaagta accttagaaa agcttggata tgatagagat gaaatgctaa
     2521 aacttattac agaaaatctg aaaggagaat tataaaatgg ataaaaatat aaacaatgtt
     2581 tctcaagatg gaatagttga atttaaaggt gtaaacaaga gttatggaac taagaatgtt
     2641 ttaaaaaata ttgatttaaa tataccaaag ggaaaaatag tcggactgtt aggacctaat
     2701 ggaagtggta aaagtacaat gattaagcta atgaatggat tattacagcc tgataatggt
     2761 gaaattatga taaatggaat gaaaccatcc atagatacta aaaaaatagt ttcatattta
     2821 ccagaaagaa cttatttaaa tgattggatg aaggtatcag atttacttaa gtttttctat
     2881 gatttttata gtgattttga tgttagaaga gcaaatgaga tgattaaaag cttagatatt
     2941 gatgtaaatg aaaagctaaa gacaatgtct aaaggaacta aagaaaaagt tcaacttatt
     3001 ttagtaatgt caagaaatgc gagcatatat atattggatg aacctatagg tggtgttgac
     3061 ccagctgcaa ggtcatatat acttaagact atattaaaaa attattctga agatagtact
     3121 ttactgattg ctacccactt aataagtgaa atagaaaaca tatgtgatga gataatattc
     3181 atatcaaaag gagaaatagt gttacaagga gacgttgaag caataagaga agaaaaagga
     3241 aaatctatag atgcattatt tagggaggaa tttaaatgtt aggtaaatta ttaaaatatg
     3301 agttaaaagc aagtggaaga atatttatac cgttatatat agccatattg atagtagctg
     3361 tatttaatgg aatatttatg aatacaaata tacttcaggt acagggaata ggaatactag
     3421 tattaacaag tctttttatg gcattaggag ttttaactat tgtagttaca attcaaaggt
     3481 ttagaaaaaa tcttttgggg gatgaaggtt atttaatgtt tactcttcct gttagcacta
     3541 gttcacttat attatctaaa tgtataacag cacttatata cgctgtactt agttttatag
     3601 ttgcagtatt cacttttgga gttcttatgc tttttggtac ttctggaatt ttactacctg
     3661 aaattttaga tttgtttaac actagtttta aatggatttc tgaaaacttt ttagatattt
     3721 tattattagt agtagtaatg tttatttcat attcatcatt tattttgtta ttatatacat
     3781 caatctcaat gggacaattg ccaaaattca ataaacatag aaacatagtg gcatttgctt
     3841 cttttatagc tataaatata gtaatatcaa ttgttggaga tgcagttgga agcatcttac
     3901 caaatgaaga tacaaatatg gtatatcatc tttatcagag tcctagtttt atgcttgcaa
     3961 tacttggtag tcttgtagtt gcaatagcgt tatttttcgc tacaaaattt atattagata
     4021 aaaaattaaa tttagaataa atgtttttat atggagctat cttttgagta atgttaaaag
     4081 atggctcttg ttatttatgt agttaattca tgtatataat ataaataagg ttttatttct
     4141 agttaaatat atacatatgt tgtataatga tattgcgtgt aaaaatttgt aagaataacg
     4201 atacacttta tataagttac atcaagaaaa tttaaaaata tatggatttg agggagaaga
     4261 tatgaaaagt tatacaaagg ctaagtggag tgtttggggt ataattacat tttcatttgt
     4321 tttagtatta tttttaagga tgtcaactgc tgttgtttca gacaatttag caaatgagtt
     4381 aggttttaat tctatacaaa tttcaaatat agcatccttc tgcctatatg cgtatgcatt
     4441 tatgcaaata cctgcaggaa ttttgattga taaatatgga gcaagaaaaa ttagcagtct
     4501 tggaataata atggcaagtt tagggtcaat tttatttggt ttgattcaaa gtatagaatt
     4561 agcatatatt tctcgtgtaa ttgtaggggc aggaacatca gtaatattac tttgtatttt
     4621 aaaaattcaa gggagatggt ttaataagtc agaatttgct tctgcaacag ctaaattttc
     4681 atttgtagga aatttaggtg gagttcttgc tacttttcca cttgtgtttt tgtctgaact
     4741 tgttggttgg agaaattcat ttttattaat cggtataatt ggtgttgtaa ttggatgttt
     4801 tatgtatatt attgttagag atacaccaaa agagtatgga ttcaatgtag atactgaacc
     4861 ctatgaaaag tcagagaagg ttaatattgt tgatggtatt aagtctgtta taaaaaacaa
     4921 atcaacatgg tataattcaa tgataatgtt ctcttttgtg ggtcttacaa gtgcatttat
     4981 aagcttgtgg ggagtaagat acataatgga cgtttatgat gtgagtaaga gtttttctgc
     5041 atttatagta tcatttttta catatggatt tatatttggt tcaataatta tggattttgt
     5101 atttgctaaa ataaggtcta gtaaatttaa tataataaaa tttggggcaa taatagattt
     5161 gtttatatgg attgtaattg tagttgtata ccaggtaaaa ccaccaatta tagttttacc
     5221 aacatcattc tttattatgg gatgtatagt tatgtcacat ctacaggttt ttaatgatgc
     5281 caagtataaa aataaagaaa tttattctgg actggcaact agtgttatta atacttttga
     5341 atttatagga agtggaatta ttaatttgat tatagctatc tctttacaag taaattcata
     5401 taatatagtt gatggatata aaaaaggttt tgtagtattt atagtattaa gtattataac
     5461 tatagtatca tctcatatag gggtaaaaaa tgatgatttt aaggctatat agtgttttat
     5521 taatagttta atttagtatt atataactta gtattatatt ttgcatatat ataacaaata
     5581 gtttatattt tcctaagatg tataattgga atataaatct tgtttactat gtacaaatgg
     5641 tattgtatta tattaaatag aaaataagaa ctttaaacta taatattggt tctaaaatat
     5701 aaggtaagga gttttttatg gatacgacga aaatagatga tataagcaag ttagatttaa
     5761 ataatttaga tattaataag atggatatgg gaggtataat ggaaagtctg ctagaatggg
     5821 ctactacaag cggagtaaaa cttataatag gcatacttat actttctatt ggatttaaaa
     5881 taataaaaaa atttgtaaac catgttatgt tattacttag taaaagagat attgatttaa
     5941 ctttaagaaa gtttttaaag tctcttcttt tgagtgtact taaagttgcc gttataatca
     6001 ttgttctgga atattgggga atgagtttgt ccagttttgc tgctgtgata gcatcagcag
     6061 gggtagcaat aggtttggct ttacaaggta gtctatcaaa ttttgctgga ggatttatta
     6121 tactattgat tagacctttt aaagtaggag attacgtaga agcagcaggt catggaggaa
     6181 cggttgaaca aataggtctt ttttatacac aattggttac tccagataat aaacagatac
     6241 ttatacctaa tggttctgta tcaaatgata gtctaataaa ctattcagca aagaatacta
     6301 gacgagttga tttgactttt agtgtagggt atgaagatga tatactacat gttagaagag
     6361 tacttaaaga tattgttaat aatcataaat taataataaa tgaaccagaa ccatttatag
     6421 gagtagtaga acatggagat aatgctatta aatttgcaac tagagtttgg tgcaagacag
     6481 aagattattg gacaatttat cttgatttac tagaagaggt taaagttagg tttgacgaag
     6541 aaggaattac gattccatat cctaaaatgg atttaacggt aaaagaatta aacaaaattt
     6601 aattttttaa tatagtgttt aaatttaaga atatcatata gatatactta taaggtttgt
     6661 aaaaaattaa taagtactgt tattttgtaa gtaaaaatat aaaaaaatgg gagtgtctca
     6721 aaatgaactt tttagttcat gaggcactct ttttgtacga aataaaatat atttttatta
     6781 tttcaacaat gaaaaagagg cttgtttcca ttttgagaca gtataatttt tatgtaactt
     6841 taattttagt aaatataaag ttttaaaatt gataaaatag gaggaatttt taatataaaa
     6901 gggtaaataa agggtttaga gggcataata tagaataaat taatatattt aaggagggtt
     6961 tttatgtgta aaaaaacgta tttattagtg gtaagtatgc ttatagcggg tttattaact
     7021 gcatgtagtg ggagtccaac taaggattca ggacaagcac aagataatac aaaaaaggag
     7081 gcaaatgcat caaataacgc tttaaaagat gaaaaaaata atgaaaattt aatggaacaa
     7141 gattttaaag tgccatacac agatgcaata aacatattta aagataaata taaggatgct
     7201 gatatagtag atttaagtct tgagagagat ttaaataaat ttgtttatac tgtggaaggt
     7261 gtagatgata ataatgaata taagatgaaa attgatgcta atacaaaaga tgtattagaa
     7321 gataaaacag aaaagttgga ttcagaagat ttaaatggag ttgctagaaa agaaaaatta
     7381 gatttaaatg acataatgac acctcaacaa gctatggaaa ttgcattaaa agaacaaaat
     7441 ggaatagtta aagaatggag tttagacaaa gatttagatg ttacttttta taaaataaga
     7501 atagataaag ataaaaatga atatgacata aaagtagatt ctaaaaaggg aactgtattg
     7561 aaggtagaaa aagaagatta gaagatttaa actatcttta aaataatttc ttttttgaat
     7621 aaattttgtt taaaatataa caagcagggt ataaagagac taaataacta ttttaggagg
     7681 agtacggtat gtcattgaaa tcactaaaaa taaaagaaaa tttatattgg gtaggttcgc
     7741 ttgaccctga tttaagagta tttgacataa ttatgtatac accttatgga actacttata
     7801 attcatatgt attaaaagga acagaaaaaa cagttttatt tgagacagtt aaagataagc
     7861 attttgacaa ttatattgaa agattaaatg atttaaatat agattttgaa aaaatagact
     7921 atattgttgt aagtcatact gaaccagacc atgcaggaag cgttgaaaag cttttggatt
     7981 tagctaaaaa tgcaaaagta gtagcttcag aaacagcaat taaatatctt aaggaaatcg
     8041 taaacaaaga ctttgaatat gttgcagtta cagatggaga tacattatca attggggaca
     8101 aaactttaga atttttctct gtacctatgc ttcattggcc agataccata tacacatata
     8161 taaaagagga taaaactctt gtaacatgtg attcatttgg tagtcactat agtaatgata
     8221 agatagtaaa tacacttgat gaaaatgaag aaaaggatta cttggatgct ttaagatact
     8281 attatgattg tataatgggt ccatttaaac catctatggt aactgctata gaaaagataa
     8341 aagatttaga tatagatact gtatgtccag gtcatggtcc agtattaact gaaaatccta
     8401 gaaaaattat agacctttat tacaactgga gtgtaaatga acaaataaag ttagaaaaag
     8461 aagttacaat ttgttatgtt tctgcacatg gatatacaaa gattatggca gaagctataa
     8521 aagcatatat tgaaaaaaat agcaactata aagtaaattt atttgatgta atagagcata
     8581 agcaagaaga tattttggca aaaatagcag tatcacaagg tgtattattt ggaacaccaa
     8641 caatattagg agatgcgtta aagcctatat gggatatatt aatatcgtta aacccagttc
     8701 ttcatggtgg aaaagttgct tcagtatttg gttcatatgg atggagtgga gaaggtatag
     8761 aaaatgctat ggagagaata agtcagctta gaatgacagc agtaaaacct tttgctgtta
     8821 actttaaacc atcaaatgaa gagattgata aattacattc ttatacaggt aaattcctag
     8881 ataagttaaa ttctactttt ggaagtaaaa agaaaactaa gaaatttaaa tgtgtaattt
     8941 gtaatgaagt ttttgaaggt gatagtgctc ctagtgtatg ccctgtatgt ggagctaagg
     9001 aagaccaatt tatagaagtt gaagaagatg aagttacttt tagaaaagat actgatgaat
     9061 atttcgtaat agtaggaaat ggagcagctg gtttttatgc agcagatgct ataagaaaaa
     9121 gaaataaaac ttgtaagata actatgattt ctaatgaaga tgagttgact tactatagac
     9181 ctgcactttc tgatggtata aatgaagaac ttggttcaga tttctacatg gaagataaag
     9241 attggtatga taaaaataat atagtagtaa tattaggaac gaatgtagat aaattagatg
     9301 aagtgaataa aactattata gtgaatgatg gagctattaa atttgataag cttgtaatag
     9361 ctacaggaag tagaaacttt ataccaccaa ttaagggtca tgatttagaa aatgtgttta
     9421 ctcttagaaa tataaaagat ttatatagtg taaaagaagc actagaaaaa tctaagaaag
     9481 ttgtagtgat tggtggcggt ttattgggac ttgaagctgc atgggaattt agactaaaag
     9541 gattagaagt agtagtagtt gaagccatgg atagcatact atcaaaacaa cttgataaag
     9601 aaggtagtaa aatattagaa caatgtgtaa gagatacagg aatagatgta agattgggtg
     9661 tagcagttga tggtattgaa ggtgatggaa aagctcaaaa agttgtattt aaagatggag
     9721 atagtgttga ctgtgatatg gtagtatttt caattggtgt aagagcaaac actcaaatgg
     9781 ttcaggatac atctgtaaaa attgatagag gtattgttgt agataagaca ttacagacaa
     9841 atattaaaga tatatatgct tgtggagatg ttgcacaagt gggaaatatt agtttagcaa
     9901 tatggccatc ttcagtagaa atgggaaaaa tagcaggggc aaatgcaagt ggagataatt
     9961 tgacatttga aagtgaagta tatccagttt cattagatgc aatgaatgta aaagtattta
    10021 gtattggtaa tatacaaaat tttgataaag aaatttcatc aaaagatgaa ggtcaaagaa
    10081 tatataaaaa attatttatg aaagatggtt cattagttgg agctatactt ataaatgatt
    10141 taagctgtac tgttaaattg ataagattaa tatctgaaaa aggtgatttt gaggatatta
    10201 tgaaggctga tatactataa tttaaataca ttaaaataaa aaggagttgt ctcaaaattg
    10261 atttttttct aatcaaattt taaattcaac tccttttttg aatttttata taaaataaca
    10321 tggaaatttt agtaatgtct gtggttacat acataggttt aattgttttt tatatggatt
    10381 gtctctggta taataaattg aattgtgttt gactgataga tatttttatt gaaagttaat
    10441 acctgttatg cgataataag gacgtgttag gggagttttt ataataacaa ttaatctcag
    10501 tttttgtgtt ccaatagtag catcaacaca ttctgatgta gtagttcttt tcttataaaa
    10561 attagttaca aatatgcttg taattatttg atatgtaaat ttaccaatat tataaatgat
    10621 gacctcgata ataaggagag tgaaaaataa tctatgaacg aaaatatttt gattgtggat
    10681 gatgaaaaag aaatagcaga tttaattgaa gtatatttaa aaaatgaagg ttttacagta
    10741 tacaagtttt atacaggtaa ggaagctcta gaatgtattg aatctaaaaa tttggatatg
    10801 gctattttag atattatgct tcctgatatt gatggatttc atatttgcca gaaaattcga
    10861 gaaaaatact attatcctat cattatgtta acagctaaaa ttgaagatat agacaagata
    10921 atgggtttaa ccataggtgc tgatgattat ataacaaagc catttaaccc tttggaagtt
    10981 gttgcaagag taaagacaca attaagaaga tatatgcgat ataataattc ttatgaacaa
    11041 cagtcgataa ttgttaatga gtatgatatt aaaggattga ttatcaataa agaaacacat
    11101 aaatgtagtt tatttgggaa agaagtagca ttaacaccga ttgaattttc tattctttgg
    11161 tatttatgtg aacatcaagg aaaagtggtt ccttctgaag aattgtttga agctgtatgg
    11221 ggagaaaaat atcttgataa taataacaca gttatggctc atatagggcg attacgtgaa
    11281 aaaattcatg aaccttctaa aaacccgaaa tttataaaaa ctgtatgggg agtaggttat
    11341 acaattgaaa agtaaaaata ttaaagtcaa tcctgacttt actcaagtga gaaataaaat
    11401 ttttatgaga acagctttaa tggcatttat agccattatt ttcatatgga ttttatattc
    11461 ttttatattg tatggtaagt tttctatctg gatagtatca ttctttgaaa atgcttttcg
    11521 tatggaatat aaagctgcat tgaatttata tcagcagatt ttccgtaata gcatggagct
    11581 cttcatgata ctagctatgg tgattgtgtt ttttataata ttccgtgttt atttaaattg
    11641 gtttacaaaa tattttagtg aaataaatca aggtattgac tctcttatca aagaagatgt
    11701 tggagaagtg gctttatctc cagagttatt ggcaatagaa aagaaaataa actcaattaa
    11761 acatatactt gaacaacgta aatttgagac acaaatggca gaacaacgta agaatgaact
    11821 tattgtttat cttgcacatg atttaaaaac accacttaca tcagttatag ggtatctgac
    11881 actacttcga gatgaaagtc agatttctga agaactgcga aaaaaatatt tatctatttc
    11941 gttggataag gcagagcgtc ttgaagattt aattaatgaa ttctttgaaa ttacaaggtt
    12001 taatttatca aatatatcac ttgaatacag tacagtaagc ttaactcgta tgcttgaaca
    12061 actaacatat gagtttaaac ctactttgat tagtaagaat ttaaaatata cactgaatgt
    12121 tacaccaaat atgatgataa ggtgtgatgt taataaaatg caacgtgtat ttgataactt
    12181 attacgaaat gctgtttatt atagttttga agatactact attgaaataa cagctgtaca
    12241 ggaggatgat tatataaagc ttaaatttat gaatcatagc aatacgattc cagaggaaaa
    12301 acttgagcgt atttttgaac aattttacag gttggataca tctcgtaatt caaacagtgg
    12361 tagagctggg ttgggacttg ctatagctaa agaaatagtg gagcttcata acgggacaat
    12421 aacagctcat agcgagaaca atataattac ctttaaggtt attattccga ttgtatgaaa
    12481 tttgtaagaa tttaagtata aaaaaatagg aaattcttta ggataaataa ataaagagca
    12541 ctcttgttat tgatataatt actgtatcaa aacaagagtg cttttatttt gatacagtaa
    12601 ttatatcaat agaaaaatgt gaaaatatat tatgtggaac agatgcaatt gtaagtgaag
    12661 gttgattgga tttgaaatag aaaaagtatt ttaacatgtt tctgtagatt gtataaaaat
    12721 aaataaatca acaaaggaga atcaaagatg aataataaaa aaattgcagt tatatttggt
    12781 ggaaactcaa cagaatatga tgtatcatta caatcagctt ttgcagtact tgagaatttg
    12841 aatacggaaa aatatgatat tataccaatt ggaattacaa gggagggtaa ttggtatcat
    12901 tatattggag attataataa tatccagaat aacacttggt ttgaaaggga tactgatata
    12961 attccagtta ttgtttcgca gaaccgtgtc aaaaatggta ttattgaatt tttaattcct
    13021 gatgctgttt ctcagaatca tattccaaat agtgctattg aatcatgtga aaatgatatt
    13081 aaatacacta aacttgattt tgtatttcct atattgcatg gaaaaaatgg agaagatgga
    13141 acagtgcagg ggttatttga attggcaggt atacaggtta ttggatgtgg tactctttct
    13201 tcagcattat gtatggacaa agacaaatca cataaacttg ttcattcaga aggtattaaa
    13261 gtaccaaaat caatattact aaaatactca gataaattta atgtggctga actgtcaaaa
    13321 gacttaactt atccattatt tataaaacct gtgcgtgcag gttcatcatt tggtattagt
    13381 aaaatttata aaaaagaaga gttacaagta gcagtagact tggcttttga acatgataat
    13441 gaagtaataa ttgaggaaaa tattgatggt tttgaggtag ggtgtgcagt tttaggaaac
    13501 gatgacttac ttgttggaag agtagatgaa atagaattgt caaaaggatt ttttgattat
    13561 acagaaaaat atacattgaa aagttcaaaa attcatatgc cagctagaat tgatttggat
    13621 tgtgagaaaa gaatacaaga gacagcaaaa attatttata gaactcttgg atgttctgga
    13681 tttgctagag tggatatgtt tttaactcca tctggtgaaa ttatttttaa tgaagtcaat
    13741 acaattcctg gctttacttc tcatagtcgt tatccaaata tgatgaaagg tattggactg
    13801 tcttttgaag aaatattgga caagataatt gaggtgtaca taaaatgatg aataatattt
    13861 tattagataa aaataaagaa gataaaatga caaatactat cttattgact aaagataaag
    13921 tttatgatgg gaatctaatt ctagtaaatg cttttttacc agttaaaaca tctgaagata
    13981 tagatttaat tcctgtagat acaaggtttc caagtattct tatgaaacgt gaagctacaa
    14041 acattttaca aaatatttta aaaagtatat gtggaataaa tgaaatagta cctgtaagtg
    14101 gatatcgtac tgcagaggaa caacaagata tatattcaag ttctctgcgt gacaatggaa
    14161 aagactttac taaaaagttt gtagcacttc caaatcacag tgaacatcag acagggcttg
    14221 ctattgattt agggttaaag aaagaagtta tagattttat ctgccctgaa tttccttata
    14281 atggtatttg taatgagttt cgtaaggcag cactacatta tggttttata gagcgttatc
    14341 agtgtaaaaa agaagaaata acaggaatat ctcaagaacc atggcatttt cgttatgtgg
    14401 gatatccgca ttctgagatt atcaataaaa tgaatctcgc tcttgaagaa tatatagaat
    14461 atattaagag attttcgtat acaaattgtt taaaaataga agataaagaa aaaattatag
    14521 aaatctttta tgtacctatt catactaaag aagaagtgag aatctctata cctgaacaat
    14581 gtgtatatca aatatcgggt aacaataagg atggttttat tgtgacattt tggagaaata
    14641 gaaaggacta agaaatggag gaacaagaat gattaggaat aaatcttatg cagggcttga
    14701 ttattttagg attatagctg cattacttat aattgcaatt catacatctc cattattatc
    14761 atatagtaaa atggctgact ttgtgttaac tagtataatt gctcgtattg ctgtgccatt
    14821 cttttttatg ctatcaggct tttttctaat ttcaacatat aattataatt gtaataaact
    14881 gaaatctttt ctaaaaaatg tattatttat ttacgggata gctattgtaa tttatattcc
    14941 cttaaatatt tacactggat atttttccat gaataattta gtgcccaata ttattaagga
    15001 cattgtattt gatgggactt tatatcattt atggtatcta cctgcatcaa ttattggtgc
    15061 aagtattgca tactttcttg taaaaaaact aggtttaaag tactcactag taatcactat
    15121 tgttatttat gtaattggta tgtttggtga tagttattat ggttttttag aaaaaatacc
    15181 actattaaag aatttatatg ggtatatttt tgaagtatca gattatacac gaaatggtat
    15241 attttttgca cctgtatttt tcatacttgg tggaataata tctaataaat ctaattatat
    15301 gtcaactaaa aatagcttga ttggatttct tatatcattc ttacttatgt tatgtgaggg
    15361 aatgttttta cataaactag gggtacagcg tcatgatagc atgtatatta tgttattgcc
    15421 atgtatgtat ttcttattta cagccttaac atttttgaaa ggttctcgag ctaggttatt
    15481 gcgaacatca tcattaatta tttatattat acatcctatg atgattgttg ttattcgtat
    15541 gttttctaaa atattcggaa tacaaactgt cttggttgac aatagcctta ttcattatat
    15601 agtggtaagt atagcatctt ttatagcttc tataattatt atattgattc ttaaacctct
    15661 aaaaaaagac ttgaaaggag aacataaggt aaataccaat agagcttgga ttgaaattga
    15721 tttaaataat ttggaacata atgtaaagat gatacaaaaa gctatgccag aagattgtga
    15781 attgatggca gttgtaaaag ctaatgccta tggtcatagt gcttttgaag tagcgacttg
    15841 tgcaaaccaa ataggtgtaa aagcatttgc agtagcaaca atagatgagg ggattgattt
    15901 acgtcgatat ggaatactag gagaaatatt gattcttgga tatactaacc caataagggc
    15961 aaaggaactt cataaataca atcttacaca gagtgttatt gaccttaatc atgctatctc
    16021 tttaaacaaa cagaaataca acataaaatc acacattaaa attgatacag gtatgcacag
    16081 actgggtttt gatgttaatg atgtgaggtc aattttaaag gcatttaaat taaaatactt
    16141 agatatgtgt ggcatattta cacacttgtg tgtttcagat agtctgaaag atgaagatgt
    16201 agactttaca aataaacaaa ttgaatgttt ttataagtta attgattttc tgttaaaaaa
    16261 aggtataaaa attcctaaag tacatattca aagtagttat ggttttttta attatcccaa
    16321 tcttaagtgt aattatgtaa gaattggaat tgcattatac gggatattaa gttctccaaa
    16381 tgatacgact aaattgcaac ttgatttacg acctgtttta tccttaaaat cacaagtaat
    16441 tcttattaga aaaatacaaa aagatgatag ttttggatat ggaagagtct ttattgcaag
    16501 cagagatagc gtaattgcta tattatctat tggctatgcc gatggtatac ctagaaattt
    16561 atcctgtgga aaaagttacg taataataaa tggttgtaga gcagcaatag tgggtcgaat
    16621 ttgcatggac caacttgctg ttgatattac agatatacct aatgtagaag ttggtaatac
    16681 ggcaataata ataggaagag ataatttatc tgaattatct gcttctgaag ttgcaaataa
    16741 ctcatgtagt atatcgaatg aattattgag cagagtagga agaaggttaa atgttataaa
    16801 gaagtaactc aattaaatag taatatttgt tgtactgtgt aatttatttt actctgacta
    16861 gacattttta gtaatattat gtctaatata tattttttat aacttacatg acaatttaag
    16921 ttataaaaag gtgatgtttc agatttttat tttgaaacat cacctttttt gttataatat
    16981 ataataaaat aattatacct taactgtaaa cttatataga ttatttactt tttaatatta
    17041 gattcatttt ttgagattgg tatataataa acatatagag tatataataa tagttaaagt
    17101 taaaaaaggg gaaaagcaat atgcagtata tacaagaact acttgatttg actcaaaatt
    17161 attgggtgtt agccacaata atcggcttat taagtgcatt tatagaaagc tttataccag
    17221 ccttaccact tgttgctata gttacagcaa atgctgcaat acaaggatta tttattggat
    17281 gtttgttatc atggattggc tcaggacttg ggacaacttc attattctta ttaataagca
    17341 gatttaatga tagtaagctt tttaataagt tgagaaatag taaaactgaa aaagcaataa
    17401 gttggatgga taaacaggga tttaagttat tatttatagc gtatgcctgt ccatttatgc
    17461 ctggttgttt agttactatc gcatcagctt ttggtaaaaa agatttaaaa gattttgtgc
    17521 cagcaatgtt ggcaggaaaa tttgtaatgt ttatagttat aagttacgtg gcaagtgata
    17581 tagaaggttt tataacaagt cctttaaaga tagcatcatt tatattattg gtgtttttgt
    17641 catggaagat tggaaataga gtaaataaaa atcttgaaaa tcataattat gatttccatc
    17701 ataaaaaaca tcacaatgat aaagatgata aaatgattta gcttgtacga aataaaacgt
    17761 atcttaaaat gatttagtac taaattattt taagatacgt tttatttttt gtattaaggt
    17821 acaaaatttt tagttctttt gtaaactaaa atatataaaa cttatttata cttagttcaa
    17881 aatattgtta tttatttttt ctacaactat aataaaatat aaaattgata tccaatcaaa
    17941 aaaataacta tgaaatttaa tagcttagat atgaattttt ctgtttttga gttagttttt
    18001 ataggtagta aagaaatgtt tatggtaatg ggataaaaca attcaatacc ttctttagta
    18061 ataaggtcaa gacacaagtg agatagatat cctattataa acccactgga caaacatgag
    18121 aaaactatat tattgttagt gaagattgtc agagatttaa aaaaactata aataagtaat
    18181 aaggcaatta gactgtgagt aaaccctcta tgtctaaacc tacttccaaa taatttgtat
    18241 attaacataa attttttact tatgtaagaa cctctcatat ctatatctgg aaataaagaa
    18301 cctaaatatg caaaataaat ataaattata attaatacta ttttatatgc aatatcgtat
    18361 ttaactaggt atagattgtt tataaagggg agtatcaaaa gtgcgaatat ataacctccc
    18421 ctttcatgtg tttcttttat catataaaaa tcctcctgaa tcgtcactat aaacattata
    18481 ttggtaaatt tagactatct tttattatat tatatagcaa acataagttt ttttcaaaag
    18541 gattttaata tttaattaat cttgagattt taaatttttg taattttcta aagcaccatt
    18601 ccaatttact acattaaacc agttgtcaat gtattcattt cttctatttt gatattttaa
    18661 atagtaagca tgctcccaaa catcaagtcc tattatagga gttaggtttt tacttacagg
    18721 gctatcctga tttggagtag tcataataga taatttgcca tctttagtag ctacaagcca
    18781 agcccaacca gaaccaaaga catctaaagc agatttttgg aactcttgct taaatttttc
    18841 aaaagaacca aagtctctat caatagcttc ttttaaagat tcagaaggta tggttttttc
    18901 tggcgtcatt atatcaaaaa agaatttatg attataagct ccacctgcat tatttcttac
    18961 agttgtagca atatctttag gtaaagaatc taaattttgc aataattcac ataaagaata
    19021 attataaagc tcaggatatt tttcaagagc agcatttaat ttatcaacat aagcttgata
    19081 atgcttatca tgatgcagtt tcattgtttc tttatctata taaggttcaa gtgcatcata
    19141 tgcataaggt aatggtttaa ccttaaattt gttattttca ggtgtaaaag caaatgaagt
    19201 tatgcactgt gagattataa ataaagacat aataactggt attaatattt ttttcttcat
    19261 aaaaatcacc tcctaatata gtatttctag aaataaaaaa aatatgaaat attaacagat
    19321 aatttataag aatttaatag caatttcatt ttaaattgaa taagttttaa agccaaagac
    19381 aaaactatga aatatagaat aagactaaag gacagaaagg aattttgatt atatggacgg
    19441 caatatttca atttattata aaggtgatat atctaaaaaa gaagatatta aatttaaaat
    19501 aaaagaatct gtaaataata caagactttc tagtagaata gagtataagg atgaggaaaa
    19561 aacttttgat tttgaaagat ttaatgtaat tgatgctatt tatggaaata aaccatttga
    19621 aaaagatgga aaagttattg tttttaatgg aaatatatat aactatcatg aaataaaaga
    19681 agaattaatt aaaaaaggac acaattttac tacaaatgat gatattgaag tcttattggc
    19741 atcttatata gaatttggta agaattgtgt acataagatt aaaggaatgt tcaattttat
    19801 aatctatgat agagaaaatg aaagtatatt tggagcaaga gatttatttg ggataaaacc
    19861 actctattat ataaataagg aaaatgcaat aattttttcc tcagagtata agtttttact
    19921 tgaatatatg aaaaatttaa atataaatga aagaagctta caaagttatt tttcatttca
    19981 atatgtactt cctgaagata ctatgataca aggaataagg ctgataccag caggtcacta
    20041 ttttagggta gaaaatggta tactatcttt aaagagatat aataaacttg agtttagaag
    20101 tagtacaaaa tttttttata ctaaaaatca cttaggcaat agagatgtga acgaggatga
    20161 tataagaaat atagtagtag attcaatagt aactcatatg gaagaggaga aagaaattgg
    20221 aacatttttg tctggtggaa tagattcatc tattataact actgtggctt cacaaattaa
    20281 tccaaatata aaatcttttt ctgcaggctt tagtgtcaaa ggatatagtg aattagaagt
    20341 agcaaagaaa acagctgata agttaggtat agaaaatatc cagattaata taacacaaga
    20401 tgaatatata aaatcattac cgaatgtaat ttattctttg gatgacccta tagcagaccc
    20461 ttcagaagta ggtctttatt tcttaattaa agaggcaggt aaacatgtta aagtagcatt
    20521 gtctggagaa ggagcagatg aattatttgg aggatataat atatataaag agtatagtac
    20581 tatgaaatca gttgtcaatt cacctactta tattaaaagt atattaggaa gagtttcaga
    20641 acttatgcca aacataaaag gaagaaatta tctttataga gctacaacac cattagaaaa
    20701 aagatatata ggaaatgcaa aagtttttga aaacagcgaa gtaaaaagat tcttttttaa
    20761 gtataaggaa aagaatatat atgaatacct gttgtctaat ttgtatagag atgcacaaaa
    20821 aaataattac gattatataa gtaaaatgca acatatagat gtgaatacat ggcttcaagg
    20881 cgatatactt caaaaaatta gcaaactttc tacagctgag caggttgaac ttagagtacc
    20941 atttttatat aaagatgtat ttgatgtagc aaaaaattta agaatggagc aaaaaataaa
    21001 taagaataat acaaaagttt tacttagaga ggcgtttaga gaaatagtcc cagaacatgt
    21061 tgttcaaagg aaaaaattag gatttccaac tccaataaga gtttggttaa aagattctct
    21121 aggggatatt gtaaaggaaa ctatttataa ttcaaatgta gatgaattta tagataaaaa
    21181 atatgcaata aaacttttag atacgcatct taaaggtcat agagataatt cgagaaaaat
    21241 atggactata tttacatttt gtctatggca tcaattattt attgagcata aaaatgtcga
    21301 gtattaatac ttttttcaat attaatcaga gggcaaaagc atgcaatgtt ttcaccctcc
    21361 tgattattat ggaataggat aagtagaact ttgattgatt agtttactat ctcaataaaa
    21421 ttattggcaa atattttgac aagattactt tcaacagcta aatttaagtt ttgtccatca
    21481 tcatatccag cggaaatcaa tcctagtaag tttccaaaca tatctaaaag agctcctcct
    21541 gaactaccac tagagattgg agctgtaaat tgtatcattt gaaccgtttc aaaatcacga
    21601 aaagctgaaa taattccatc tgaaactgta ttaaaaagac ctaaagggct tccaattgca
    21661 actatttttt gacctctcac aggtttttta ctgactttaa caggtattgg cttgcatttt
    21721 ctattaacct ttatcacagc taaatcataa tctgaatggt attttataat ttggtaagat
    21781 gtataaactt tatcatcatt ttcaaatcta actaaaaatg aatacccatc attgactaca
    21841 tgaagattcg taagtatata accttcatta tttattacta ctccagaacc gctcttaaac
    21901 ggtttatttt cgttattgca cacaacaatc ataacaacag aagctgataa ctttgcaagg
    21961 tcttcataag aaagttctac ttcctcatct tgtacaattt ttttagatgt tagatttgtg
    22021 ttattaagtg tatttttaga gttaggttta ttcgtagtag gagaagtctt attggtattt
    22081 tcactgtaat ttatatttgt aatatgcctt tcaaaaggta tttctaatat atttggagtc
    22141 ttcctagaaa gttcaaatag agtagaaaat tttgaacatt caggatataa atcaagaata
    22201 tctttatttg gatttatata taaaatatca cacccaagtt gtgataaaaa atataagaaa
    22261 aaaagttctt gccttttaat atttccaaag tatactatct ttggtgaaat agtagtcttc
    22321 accttgttat aaaataattt aggtagaaaa tagtccatcc aacacaaaag tttaactgca
    22381 aagtttttga tgatagagga tgtacaattt ggcatacttg tggaaaataa atctaaagta
    22441 tttttaaatg caatcttttt agtccaattt aaggtgtcat tttctatttt acatggaaag
    22501 tcgatattat ataaatccca actgttaaac ttagaactat cataaatatt aaaaatttta
    22561 agcatcttat ctgtttgttc tttgtcaaaa tttattttta gattgtttaa tctaatatag
    22621 ggaattgtag gattattaaa aaaataagta tctatatttt taattttttc tatgtattta
    22681 tcaattgaat catcaacacc tatatatctt gtaaagtaga cttctttaat atatgaagaa
    22741 cccttatttt tataggtagt atcagatata aattcaatta gaggttcagt agactttttt
    22801 attgaaatct ttaatggttt aatagtacaa atcatatttt ttctcctata ataagatgat
    22861 ataatctatt ataaaacaac ttaaaacaat atagtacaat aattttgtag tttcaaaata
    22921 aaatatgtac ttgtttatat aaatgatata taattacata ttaaacatag cttttataca
    22981 tttaataaag tatgtaaaat ctatgtaaaa agtaagaaat ataaacattt ttttaattat
    23041 attttaaaaa tatcaagcaa gttttcttca caaatgaata aaaaatgtta taatatgatt
    23101 aaagaaaaag ataaataatt agcaagattt gtttagctaa aattaacaag ggagcgataa
    23161 attatgggta taacattatc aaaaggccaa aaggttgatt taactaaagg aaatccaggt
    23221 cttaaaaata ttttagtagg tttaggatgg gatactaaca agtatgatgg tggatttgac
    23281 tttgatttag atacagcagc atttttaaca ggagcaagtg gaagtgttac taatgatgga
    23341 gattttgtat tttacaacaa tttaaaacat acatctggag cagttgagca tctaggagat
    23401 aacagaactg gcgaaggtga tggagatgat gaacaaatag ttgttgattt atctaagatt
    23461 cctggagaaa taagcaagat ttcttttact gttacaattc atgatgcaac agaaagaaga
    23521 caaaattttg gacaagtaag taattcttat ataagaatag tagataaaga cacaaatgaa
    23581 gagttaataa agtatgaatt aggagaagat tttagtatag aaacagctat agtagtagct
    23641 gaaatatata agcacaatgg agaatggaag tttaatgcac taggttctgg ctttgaaggt
    23701 ggtcttgctg cattatgtgg aaactttgga ataaacttat aatttcaact ataacaataa
    23761 actaaagggg gatattttcc atggctatag tattaaaaaa aggacaaaaa atcgatttaa
    23821 ctaagggaaa tccaggtctt aaaaatataa aacttggact aggatgggat acaaattcat
    23881 ttgatagtgg ctatgattat gatttagatg taagtgtttt catggttgga gaatctcaaa
    23941 gagttgaaaa agatgaagat tttatattct acaataactt aaaacatcca tctggagcag
    24001 ttgaacactt aggtgataat agaactggtg aaggtgatgg agatgatgag gaaatattag
    24061 tgaatttaga gacaatgcca aagcatatac aaaagatagc tgtaacagtt acaatatatg
    24121 aggctgctga aagaagacaa aattttggac aagtaagcaa ctcatatata agagtattaa
    24181 attctgaaaa tgatgaagaa atattaagat atgacttagg tgaagaattt agtatagaaa
    24241 ctgcaattac agtatgtgag atatataagt ataatggaga atggaagttt agtgcagttg
    24301 gagcaggatt tgaaggtggc ttagaagcac tttgtagaaa ctatggctta aatgtttaat
    24361 ttaagcaata taatatatta aatataaata acctatacaa ataatgtata ggttatatta
    24421 tataaaattt tgtggaggtt tagtatgtcg attaatttat caaaaggtga taaaatagat
    24481 ttaaaaaaat ctaacccagg actgagtaac atacttgtag gtctaggatg ggactctgtg
    24541 caacaatctg gaggaggttt ttttaaatcg ttatttggtg gaggacaagc tgatatagat
    24601 tgtgatgcat ctgtatttat gcttaatcaa gaaggtaaat taagtggaat aaaagattta
    24661 atttattttg gaaatctaaa aagtgcctgt aaatcagttt tgcatacagg agacaatcta
    24721 actggtgagg gtacaggtga tgatgaacaa atcctagtta atttggataa agttccatca
    24781 aatattcata aattattgtt tgtagtaaat atatacaatt gtgtagatag aaaacaacac
    24841 tttggaatga tagaaaatgc ctatataaga gttgaagacc aaggtaataa gaaagagata
    24901 gcaaaataca atctatctga taattattct gaaaaaacta ctcttatagt aggagctata
    24961 tatagaaaag atggaagttg gcaatttaaa gcaattggtg aaggaacaaa agatgcgggt
    25021 cttaaagaag taatgcaaaa tttagacagg attgagtgtg catatggaat ttaataatga
    25081 tatcaaatat tataatgtag ataaagaaga agttacaaaa gacttatctg ttaatcctga
    25141 aaaaggttta tcagaatcag aagtcaagac tagaagagaa aaatatggtt tgaatgaatt
    25201 tactccaaaa gaagagggta gtttttggga tgatttaaag gaaagtttaa gtgaaccaat
    25261 gatagttatc ttgattattg cagcagtagt aagtgcagta attggggaaa ctcatgatgc
    25321 aatagggatt gtaggtgcta tagcaatagg tatagcaata ggtatgatta cagaaggtcg
    25381 ttctaagaaa gcagcagagg ctctttctaa gttgactgaa aacatagaag taaaagtatt
    25441 gagagatggg gaagtacatc aaatttctaa gagtgaattg gtaccaggtg atatagtata
    25501 tattgaaact ggtgacatgg tacctgctga tggtagactt atagaaagta taaaccttaa
    25561 aattagagaa gatatgttaa caggagaatc tgatgacgta tctaaaaagt gtgatgtagt
    25621 agtttctatg gaagagatag aatctaaagg agcagtagta gtacaggagc caatacctgc
    25681 aaaacagata aatatggtgt ttggtggtac acttgtagct tatggtagag gagctctagt
    25741 tgtaacttca attggagata atagtgaaat gggtaaaatt gctcaaaact tatcagaaac
    25801 agatgagcaa actccacttc agataaaact tggaaattta ggtggtttga tagccaaagt
    25861 ttctagtgca gtagcaggat tactatttat atttatggtt ttccaaatga taagtaagaa
    25921 tgtattaaat gtagatatgt ctggaatatt accgttttta gaatcaatag acccagttaa
    25981 aacagcattt acagtatgtg ttgcgttgat agttgcagca gtgccagaag gtcttccaac
    26041 tatgataaat atgacacttg caataacaat gcaaaaaatg gctaaaataa atgcattagt
    26101 taccaaaaaa gaagcttgtg aaacgatagg ttctgtttct gtaatatgtt cagataaaac
    26161 tggtacatta actgaaaata gaatgactgt agaagttgca tatgttgatg gcagatatat
    26221 tgaaagttca gaagaggaaa taaatagtta ttttgaagaa aactgtatga taaactctac
    26281 agctgatgta gagcacaatg atggtgatat taagtattta ggaagtgcta ctgaatgtgc
    26341 attgctttta tattacaaaa acgtagatta tagacaagtt agaaaaaatt ctaatatagt
    26401 agctcaaaat ccttttactt cagattcaaa gagaatgact tctgttatag gtcgagataa
    26461 tcatcatgtg ctattatcaa aaggagcacc agaagtatta cttgaattat gttcacatat
    26521 tcaaagagga aaagacatag ttccattaac tgaaaatata aaacatgaga ttttagaaga
    26581 aataaagaaa ttgcaaataa aatcaatgag aactttaggg tttgcatata aagaaatctc
    26641 acaagctgaa gaagaagcag cagtaactgc tgaaagtgat gctatgaatg taagtgcaat
    26701 ggaaaacaat ttagtattta gtggttttgt tggtataaga gacccactta gaaaagatgt
    26761 tatagagtct gtaaatacag caaataaagc tggtgtttct gtaaaaatgc ttacaggaga
    26821 taatataaat acagcaagag ctataggtga agagttaggc ctattaaaaa ataatatgag
    26881 agtggttgaa gcttcatata tagatacttt agatgatgaa gaattaaaac aagagataca
    26941 aagtatttca atcgtggcaa gaagtaaacc agatagtaaa atgagaatag tttcagctct
    27001 gcaaaagagc ggagatgttg ttgcagtaac aggtgatggt ataaatgatg ctccagcact
    27061 ttcaaaagca gatgttggta tagcaatggg tatatctggt acagaggtat ctaaaaacgc
    27121 agcagacata attttaactg atgatagttt tagtaccatt gtaaaaggta ttaaatgggg
    27181 tagaggaatt tatgataact tccaaagatt cgttcaattc caattaactg taaatgtaat
    27241 tgcattttta gtggctataa tatctcaagt tatgggacaa gagatgccat ttactacaat
    27301 acaattatta tgggtaaaca tcattatgga tggaccacca gcactagcat taggtttaga
    27361 gccagttagg gaccatgtgt taaacagaaa acctgttgat agaaaagcca atataatttc
    27421 taagtcaatg gtgtatacaa tagtattaaa tgcattttat ataacagcaa tactaatgtt
    27481 acaatctaca tttaatttct taggagttga tgaagcgcat aagggaacag taatgtttgc
    27541 gttatttgct tttagtgcac tgtttaatgc atttaactgt agagagttta atgcagatag
    27601 tatcttccct aattttacac ataacaaatt agcactacaa ataatagtgt tgacaggaat
    27661 tgcacaagta atatttactc agctattcca agatttcttt aactctgtag caatggattt
    27721 tatgacatgg gtaaaagttt tatgtgtagc agcaagtgta attgtggtta atgaagttgt
    27781 aaaatgtata ataagactgg taaaaccaaa aaagactgct taaaacaaat tttttagaag
    27841 aaaacagact gactcaaaat atgaattttg ggtcagttct gaataaacat ttaaattggg
    27901 aggatagtta tgggtgactc aaaggtattt gaaaaaatat tctccagcca atcagataga
    27961 ggaaattaca ctccttcaaa gggatatcta agttatttta tttcgtatat tggtttagaa
    28021 gatgaagttt tgtacaatct cgagatattt aaaacaaaac aaaatattga tagcaaaaaa
    28081 gatattgctt tatttactga tgttatagca aatcctagtg attttgatat tataaattat
    28141 tttaaaagtg gacttcaaaa atatagaact agcatggaag atgtagatat aaatattttg
    28201 ggatttgaag agattgatta taaaataaag caagcaatgg atagagtgtt aaaagaagag
    28261 gaaaaagaat ttactaatga tagagttaaa caaaatttta tagtgaaaat aatggcatgg
    28321 ataaaaattt atatcggtgc tttagatata aataaaaatg aagctccaaa agtaatattt
    28381 tatggagata taaaaaaaca tgaagtttat ttactcttaa tattatactt ggcaggtttt
    28441 gatgttttat atcttaatcc aaattcaaag agtaatatag atattttaaa atcagaaaga
    28501 tacaatatag agtttgaaga agccaacata atagaagaaa aaatttcttt tgaagaaagg
    28561 gttattttag gggaaaaaat agataaaagt tctgtaaaaa aagcttttac tgttggggca
    28621 gaagcatcaa agcgtataag tgaggaattg ttaaatgatg caggatttat aaaatcttgg
    28681 caactccaag atagaaaaat aaaaaatcta cttctaagtt caactgttga tgaaatatca
    28741 atatactgga atcaaccact taaattaaga ccaggattta agtttaatga tgctattgta
    28801 gaagctccaa attttctttc taagattaat ggaatatata acgataagaa tgaatatatt
    28861 aagtttttag atttattaag agattcggaa agttcaacat ttattgaatt taatggagat
    28921 gtagatagat tttcaaaagc ttttacaaga gaagctttta gtttatcctt tttattggat
    28981 tcaaaaggtg caatagataa aaactctgtt ttaaataata aagattattc catatcaaca
    29041 ctagcattga atcaacaaat tatgatttta gagaaagtgg aagagttgtt agaaggaagt
    29101 atgtttttga atggtttaag tggagaagac aagataaaag gtctatttac agtacttcac
    29161 atggataaga aatttgttca tatgatgaac aatttcgatt actcacttat aaacccaaaa
    29221 ttgattatat acatgtacaa gagtattgta tttgataaag aaatagtttt tcttatgctt
    29281 ttacttagta agattggatt tgatattatc atattatgtc caggaggaga aaacaatata
    29341 gagaatgtta taaataacca attgattgat gtacacagat tggataaaat ggtatatgac
    29401 cttaaattaa attcgcttga aaatgatata cctttactta aaaaaatttt tggaaagagg
    29461 agacgatttt agatggggat aaatataaat agtttttcaa aagataagaa ctctgtagag
    29521 gttactgaga atttgcctac agcagttcaa gaagaaaact tcgatataat ggaatataca
    29581 aataataaaa aaaatgaact gagaaagtct aaggaagttg aggcattgac atctcttata
    29641 gaagtagaaa atccagatac gattttacag tttggtagga aagcatctga aggagtagca
    29701 agagtttctg atagcctttt aaatacaata aaattaaata gaaatgaaga aaattctaaa
    29761 atgctggttc atttaactaa aataatggat aagtttgatt tagacgattt tcaagaaact
    29821 aaggaaccaa atttcgttca aaaacttttc aaaaaagcta ataatgccat tgaaatgatg
    29881 tttcaaaagt atgaaacatt gggaggagaa gttgagaaaa ttcaaattga gcttgagcaa
    29941 tacgaaagag atatagcact ttctaataag caaataggtg ctatgttaaa tgaaaacttt
    30001 gagttttata atgaacttca aaaatatatt gttgctggag aaatggcaat agaagaaatg
    30061 gataatgaaa tactaccttt ctttaaacaa aaatcagaga ctagtggaga tcaaatggat
    30121 gttgtcaatt atcaagaact attaaaagtt tatgatatgt taaatcaaag agtctatgat
    30181 ttaaggatag cagaaaatat agctatccaa acaataccta tgcttagagg tatgcaacat
    30241 aataattatg gtttaattag aaaaataaat agtgcatttg tcgtaacttt accagtattt
    30301 aaacaatgtt tatctcaagc tatattactt aaaaagcaag aattacaagc aaaatctcta
    30361 aaagcacttg atgacaaaac taatgaatta ttgcttagaa atgcacaaaa tgtaagtact
    30421 caaagtgcac aaatagctag aatggctgga acaagttctg ttcaaataga aacattggaa
    30481 aaaatgtaca atactatcaa atctggtata gatgaaacta tgagaataga agaaaacaat
    30541 agagctttaa ttaaggataa tacaaagaga ttggaagaat taaatacgac tataatttat
    30601 aataagtagt attatattaa gggctaattt gtataaagtt ggccctttct atgtatatga
    30661 ggaggctaga cattgaaata tttcgattat atctgcaaag atgatttaga gagaattttt
    30721 ttgaaggagc cagaagattt tagtgctaag actgaaaaag atgtgttgaa atatgcactg
    30781 ggcgcttttt tatacgttcc agctactcaa tataatatga tttataagag tattataggg
    30841 gatgttaagg gagttagacc attagctatt tgtttagaag atgctgttgg agtaaatgga
    30901 gagttagagg ctatagagaa tcttagatta attttaaaaa atattagcaa tgaaagtata
    30961 actaataaag atggaatacc actaattttt gtaagaataa aagatgtgga gcaactgttg
    31021 agaattaaag agattataat taaaaatagt cattttataa caggaatatt aattcctaaa
    31081 gcaaattcgg agttaattga aaattgtata gaagctttag attctatgaa tcttcaagat
    31141 atgtatgtta ttccgatagt agagacaaaa gaatttatat ataatgaaaa aaaagaactt
    31201 agttttacta atttgtacaa tgctatttta agacataagt caagaatatt aagcattaga
    31261 gtaggcataa ctgatatatt agggatgtat ggtataagga gagataaaaa cttctctata
    31321 tataataatt tgatttgttc ttcatttata ttagatataa tcacatatct tcaaagacca
    31381 gagttagata tacctataag tggtggagta tcagaatttt ttgatatgac gaataaggat
    31441 ataagaaata agtatataga agaaatatta ttggataaat atcatggatt aattggaaag
    31501 accgtaattc atccaatgca aattcaaatt gtacaagcgc tatctacagt atcatatgaa
    31561 gattttgctg atgctttgga tatactggat agtacagaca gtaagtatgg tgtgagcaag
    31621 ggtgtattag gagagagaat gaatgaaaca aatccacatt ttttatgggc taaaaaaaca
    31681 ttgattctat ctaaaatata tggtgtatta aataaaggag tagactatga agaattgctt
    31741 aaattttaat tgtgagattg acattattga taatccattg aatttagaac taaatgattt
    31801 tttagacgta ggaataagaa ataatagcaa aagacgtttt ttgtttatct caaagaaact
    31861 cggtaaacat ttagcatgta agccaagtga gatggataat ttgggaaaat taatggctac
    31921 aatttataat gaaaaaaata aggaagaatc aaatggtact gttatagctt ttgctgaaac
    31981 tggtacagct gttgggcata gtttttttga ttacttatgt ggagattatg aatttattca
    32041 tactactaga gagcagtttg acgagttaga aaatctggaa tttttagagg aacattccca
    32101 tgcaacagac cagaatttat actttggtat gcttaacaac tttaaatttg gagatgagat
    32161 aatattggtt gatgatgaga ttaccacagc caatacatgc atgaatataa ttaggaaaat
    32221 tcaaagcatt tatcctaaaa agaaatacac tatatgctca atactcaatt gggtaagtga
    32281 tgaaaattta agtaaagtta ctcgtttaga aagagagcta gattgtaaga tagattttgt
    32341 atacttattt aaaggaaact ttaaatttta tatagatgaa gatattattt caaattacaa
    32401 cgataattat agtattacta aattagagac tatttcaagt gataaagtaa atttagtagt
    32461 aaatcatatt tatgttgatt tagaagatta tattggaaat aagaaatatg taaaatatac
    32521 aggaagattt ggaattaata gaagagaaca atttagatta aaagaaatcg ttaagagaga
    32581 aagtaaaaaa ataactatgg ccagtaaaga atctttaaaa aatgaaaaag aaaagattct
    32641 atttttaggt actgaagaat ttatgtatat acctatgctg tttgcaaaag aaaaagaaaa
    32701 taaatatgat atatactatc attcaacaac aagaagtcca attattaata ttgataagaa
    32761 taattatcct attaaaagta aatttatgct aaagagtttt tataatatag atattcaaaa
    32821 ttatatttac aatatagata aacataattt ttctaagtgt tatttatttg tagagctgaa
    32881 taaaagtgaa aattcttatc tagaatttat tgatataatt aaaaaaacaa gtataaaaga
    32941 attaactata gtatgttgta gctaaagtgt tttaacagga taaaatatat cacgatttta
    33001 agataggagt tgagtcatat gagagatttt agcacgtatt ctaaagatga tgttattttt
    33061 ttattaaaag atatatcaaa tttaattgaa gaagaaggaa ataaagaaag agaattaaaa
    33121 atacaaaatg gtagacatta ttcagaaatg ataccaatag agtatgaagt tagtgaagat
    33181 tatctgaatt tatatcatga gaaactaaga gaaaataaag ataaacttgc attttctata
    33241 ggagttatgt gtgaaaaaat aattaaaaaa aatggaaatg atgtagtact agtttcactt
    33301 gctagagcag gaacacctat tggtattttg gcaaaaagat atataagaag caagtataat
    33361 ataagtttac ctcactatac aatctctata ataagagata agggaataga catgaatgca
    33421 attgaatata tagtaaagaa tcatccgagc tcaaaaattc aatttttaga tggatggact
    33481 ggtaagggaa ctatatcaaa agaacttgaa aaagcatgtg atgaacttaa tgttaaattt
    33541 aaaaaatatt ttgactcaac acttgctgta ttagctgacc ctgctggata ttcaggactt
    33601 tatggaatta gagaagattt cttaatacca agtgcatgtt taaattcaac cgtttctggt
    33661 ttagtaagta gaactgtact aagagaggat ttaattggta aaaatgactt tcatggagct
    33721 aagttttata gacatttaaa agataaagat gaatctatga actatatcga aactatagaa
    33781 gaatgtttta aaaatcaatt taagaatata tctgatgaag ttgaaaactg ggaaagtgac
    33841 attataacaa gagatggata ctttgatgtt ttaaatataa aagaaaagta taacataaca
    33901 gatattaact ttatcaaacc aggtgtaggg gaaacgacaa gggttttact tagaagagtt
    33961 ccgtataaga ttcttgttaa agacttaaat gataaatctt tagaccatat attcatatta
    34021 gctaaggaga aaaatgttga agttgaacaa atggatttaa aagcctataa atgttgtgga
    34081 ataataaaaa atatgaaaga tatttaggaa atatattaga caggagaata ttgaatgatt
    34141 gttttttcag atttagacag gagtatcatt tactccaata aatttttaaa tgctgatagt
    34201 aaatatacta atatagaaat ttatagggaa aaagaaatat cttatatatc tttagatact
    34261 ataaatttaa taaagcaaat acaatattat ggtatgttta tacctacaac tactagaaca
    34321 gtggagcaat ttaagcgtat agaatttaat aaatatggga tttattttcc ttggagtata
    34381 acttcaaatg gaggagttat acttaaagat aatgaaatat tgaaatcatg gagtgaaaaa
    34441 atagataaac taaaatctga ttatgagccc attgaaagta tgattcataa atttaaagac
    34501 tatttgaatg tagatggaat aactaatttt aaagttgcag aagatacatt tttttatata
    34561 gttgttgatt tatcaagatt taatttggat tcaatcaaag aatatacaaa tatattggaa
    34621 tcaaaaaatt ggaagtttta tgtgagtggc agaaaaatat attttatacc aaaagaaatt
    34681 tcaaaagaaa atgctataaa atacttgact aaagaattag gaatagaata tttttatgca
    34741 gttggagatt ctattatgga ctatggtatg cttaatatct caaataaatc ctatgtattg
    34801 aagcatggtg atataaataa aaatgaaata gaaaattcat ttatatcaag ttctttcaat
    34861 ggaatgagtg gaactgaaga gatactatct aacattttag acgagaactg cctacttaac
    34921 atataatgac tgatatataa ataataaact agaaaaatct gaaataaatt atagtaaagt
    34981 ttatcatact aatttatttc agatttttat caatttatat ttaagtaaga actcaatatg
    35041 ttaaaatgaa ggtggagtca ttgcccagac tgagatacat acctcatcat cataattttc
    35101 atacacatgt ggtatagtac tgttaaaata aaaactatct ccttcctcca atagatattc
    35161 cttatttcca agtgttatct tcattttacc ttttattata tagccacatt cttctccttc
    35221 gtgtgtcaca aattcactgt ttttttgtgt atgaccatct agcgtaacaa gtataaattc
    35281 tatttttttc ccagataaat taggaacaag aagttcataa aattttgtag aatcattagt
    35341 tactattttt tttcgattat ttttctttac tactatatca gagtcctctc ttccaatctc
    35401 ttcaaaaaaa taacctatat ttatatcaag agcttttgat attttccaca ttgctacaat
    35461 agatggaaca accatattac gctcaatttg acttattaag cctgtactta aatctgaatt
    35521 ttttgctaaa gtagagatac ttatattttt agattttcta agagatctaa ttttgcttcc
    35581 aacatctata ttcattagat acaaaccccc tcagacatga ttataacata taatttataa
    35641 agattaataa aataaaaaaa aagttttaca atacaagtat tttttatgct atattgaatt
    35701 ttctgaaaag atgcattata atagtattaa tattcaatat attgaatatt gataacgata
    35761 ttttaattta atgaaatgga ggtaattaag atgaagagtt ttgtaagtcc tgataatatt
    35821 tgggtattat gggcagttgt aactggatgg gcagcattta gtatttattt ggaacaaaag
    35881 tacaattggg catcaaaagt gtcgggagca ataatagctc ttgtaggagc aatgttatta
    35941 tcaaatctaa atattatacc agtagagtct gttgtatatg accaagtatg gggatatgtt
    36001 gttcctttag ccatagcttt attgttatat caatgtaaca ttaaaaagat atggaaagaa
    36061 agtggaagat tgctcattat atttcttgta ggttctgtag gaactgtact tggagctatg
    36121 attggatttt tagcattaaa aaatgtagta ccagatttaa atattgttgc agctatgatg
    36181 acaggttctt atataggtgg aaatgtaaac tttgctgcta tgtcaggggc gtttgatgcg
    36241 ccaggagaat tagtatcagc aacagttgta gcagataatc ttttaatggc attatatttc
    36301 tttgtcttaa tagctatacc atcaattgga ttttttagaa aacattttaa acatccacat
    36361 gtagatgaaa tggaaagtat aggtataact gaaggagaaa cagtagcagc aaattattgg
    36421 ggaagaaaag aaatatcttt aaaagatata gcaatagcca ttggtagcgc ctttataatt
    36481 gtcgcagtat ctgtagaact atcaacttgg tttaaagcta taataccatt atcaaatcct
    36541 tttttagcaa tgttaaatac tttatttgga aatcaatatc taataataac tactttgact
    36601 atgttgtttg ccacatttat gcctaatttc tttggtgata taaaaggagc acaggaattg
    36661 ggtacatttt taatatatat attctttgtt gtagtaggtg ttcctgcttc aataacatta
    36721 ataattcaaa aatcaccatt actactttta tactgcggaa taatggtact aataaacatg
    36781 ctagttacat ttatagttgc aaaaatattt aagtttagtt tagaagaagc tattcttgca
    36841 tctaatgcaa atattggtgg tcctacaaca gcagcagcta tggcaatatc aaaaggatgg
    36901 tctaagctag tagggccaat acttatagtt ggaacatttg gatacatagt cggtaattat
    36961 tttggtcttt tagtaggaaa tattttgata taattttacg tgaaaaaaag ttgtttttta
    37021 gaaagatttt tatttaattg gaggttgtta tgattttcga tggacattca gatacttggg
    37081 cagatgtaac aataagaagg cttaacgggg aaaaagatgt atttagaaga atccatcttg
    37141 ataattttaa aagagcaaat atagaaggag ctatttttgt aatatggata gacccaccat
    37201 atgataaaga gcctgaaaaa agagttaaac aaattataag ctctataaaa gatgaaattg
    37261 aagatatgaa agatgttgta aacatagtaa aaaaatattc agatatagat aaaggtataa
    37321 gtgaaaacaa aataaatatt ttaattggtc ttgaaggatt aagccatatt ggaagaaata
    37381 tagatttact agattatttt tataaagaag ttggtgcaag acatgcatca ttaacttgga
    37441 atgaacagaa tgaacttgct acaggatgtt taggggatga aaatcgtggt ttaactgaaa
    37501 taggtaaaaa agcagtcaat aaaatggaaa aattgggcat gatagtagat gtgtcacatt
    37561 taaatgaaaa atcattttgg gatttaatgt ctgttgcaag taagccagtt atagcttctc
    37621 attctaatgc taggagttta tgtgatgcta aaagaaatct tacagataat caattaaaag
    37681 aaatttcaaa tacaggtgga ttagttggaa taaatagttt tagaggattt actaatagta
    37741 atcctaagaa gcaagattta atacatttgg ttgaccatat agactatatg gtaaatctta
    37801 taggtataga acatgtatct tttggttttg attttagtga ttacttaggg aaagagacta
    37861 taggttcttt tactggaaat gctagtgtgc caagtgtaga tggtcttgaa agcacacata
    37921 cagcacagaa catagtagta gagcttgaaa atagaggtta taaaaagaaa gatatagagt
    37981 taatctctag tggtaatttt tataggctct ttaaagatat actaaaataa tataataaag
    38041 aacttaaatt tataaaatag tatatttata caaataatat ctacatattt aaattggcgt
    38101 tcataattta ttaaattaaa ttttaatgtt actagaagtt tcaattctag taacattttt
    38161 tatgttaaaa acagatatag atatgtattg acaatgataa ttattattga taaactataa
    38221 aagtgaaaat gataataatt ttcattttaa ttaataaaag atttttaagg agggagaatg
    38281 aagaaaaggt atttagtcat aggaataata attttgtctt atttttcttt atttatagga
    38341 gctgaagata taaatataat gcatatattt gcaaaaaatc agcataaact tatgatattt
    38401 atcatgagta gaataccaag attaataagt atattgattg ctggtgtagg aatgagcata
    38461 gcaggtctta taatgcaaca gataagtaag aataaatttg tatcacctac aacaggtgct
    38521 acaatagatg cagcacaatt tgggatagta atatgtatgc ttttagtacc aactgcctct
    38581 atttttacaa agacaataat agcatttgta ttttctcttg tagggacttt tacgtttatg
    38641 aaaataatag ggaagttaca atttaaaaat ataatctttg taccattagt aggtataatg
    38701 tttggaaata taataggttc tatgacggat tttatcgcgt ataaatacga tttgagtcaa
    38761 aatgttagtt cttggatgca aggtgacttt tctatgatac ttaaaggaaa ttatgagata
    38821 ttatatatca caatacccct tataatatta gcatatattt atgcaaataa atttactgtt
    38881 gttggtatgg gtatggattt tgcgacaaat ttaggtctta gttacaaaag aattgtcaat
    38941 ataggtctga taatagtagc acttgttaca gtatgtgtag tcgttacagc tggtaatatc
    39001 ccttttatag gtctaatagt tccaaatatc gtatccttgt acatgggtga taatataaga
    39061 gcaagcatat ggtatactgg tttacttgga gcgatatttg tacttatatg tgatattttt
    39121 ggaagaatta ttatttatcc atatgaaata tctatagggc ttacagttgg agttatagga
    39181 agtatactat tcctttattt aatattaagg aggaacgtaa atgaagctta gtctcaatca
    39241 aaaaatttac tttgtaagta tattactatt catatcaata gcactttttg taatttatgg
    39301 aatagacatg aatcacttag aatatgcatt atcacaaaga ataccaaaaa tatttgctat
    39361 ggtattaggt ggaggatgta tagcatttac tactgtagtg tttcaaacaa ttacaaacaa
    39421 tcaaatatta acaccaagtg tactaggttt agattcattg tatgtaatga ttcaaactat
    39481 aatagtattc atatttggtt caagcagtag tctgattata aatgaaaatt ataattttat
    39541 tattaatgta gctatgatga ttggagcttc attgttatta tataagacgt tatttgaaaa
    39601 aaataaaaat aatattttct ttttaatcct agtaggaatg atatttggaa ccttatttaa
    39661 aagtgcaaca acatttattc aggtaatgat agacccgaat gaatttttag ctttacaaac
    39721 tagtataatg gcaagtttaa acaatataaa cactaatgta ttgttaatag catttataat
    39781 aattattgcc ataatacctt ttatatatga tgaaataaag tatttagatg tattgtcgtt
    39841 aggtaaagaa caagcaatta atttaggtgt agattttgat aaagcagtta aaaaaatgat
    39901 aatattaata gcaattttag tctcaatatc aacagcactt attggaccta tgacattttt
    39961 aggactttta cttgctaata taacaagaga aatttttaaa acatataagc atacatactt
    40021 gatttcgggt tctatgttga tagggatgat tacattgata ataggtcaat tttttataca
    40081 acatgtattt aagtttgata caactttaag tgtagtaata aattttatag gaggaattta
    40141 ctttattcaa ttactattaa agggggcaaa tagatgatag agataaaaaa tatatttaaa
    40201 agatataaga ataaaaatgt tgtagatgat gtctctttta gtatagaaaa gggaaagata
    40261 acatctttta taggaccaaa tggagctgga aaaagtacag tactatcaat agttacaaga
    40321 cttataggag gtgatggtgg agaagtaata atagaaggaa aaagcttgac taattatagc
    40381 aataaagaat tagcaaaaaa aatagctatt cttaaacaat ctaacaatat aactttaaag
    40441 cttacaataa gagaattagt tggatttgga agatttccat atagtgaagg taatctaact
    40501 aaagaggatg aaaattatat tgatgaagca atagaatata tgaagttgac tgatatacaa
    40561 cataagtatt tagatgaatt gagtggaggt caaagacaaa gagcttatat agcaatggta
    40621 atagctcaag atacagagta catattactt gatgaaccac tgaacaattt ggatatgaac
    40681 cattccgtac agatgatgaa ggtacttaga agtctttgtg atgagttaga taagactata
    40741 gtattagtaa tgcatgatat aaattttgca tcatgttatt cagataatat agttgcatta
    40801 aaaaatggaa aagttgaaaa agtaggtcgt acagatgaga tagtaaatga aaaagtttta
    40861 gaagatattt atgagatgaa ttttaacatt aagaatataa atggaaatcg aatctgtatt
    40921 tatttttaaa atattgtata tgaaaataaa cttgctatat aattttttat aaagttttta
    40981 aattaaaata aagattaata ggagagagga aaatattgat atgaataaaa aagcagcaat
    41041 agtagcagca gtagctatta taggattagt gacagtgttt gcattaggtg gaagtaaaaa
    41101 gaatgaatca aaaacatcag aaaattcaaa taataccata aagataactc ataacttggg
    41161 agaaacagat gtaaaattaa atcctaaaaa agtagtagta tttgattatt cggctttaga
    41221 tacaatggat gcattaggtg tagctgagaa tttagtagga cttccaaaag caagtttacc
    41281 agcatcttta gaaaaatata aggatgacaa gtatgcagat ttaggaggat taaaagaacc
    41341 agatttagaa ggtataaagt cggcaaatcc agatttaatt ataataaatg gaagacaaga
    41401 agatttttat gagcaattgt caaaaatagc accaacaata agtacaagta aagatgataa
    41461 gaagtactta gaatctgtta aaaataatat agacaaaata gcaaaaatat ttggagtaga
    41521 agaaaaagca aatcaagaat ttagtaaaat tgaaaagaaa atagaaactt tgaataaaaa
    41581 agtaacagat aaaaatttaa atgctttaac tataatggta aatgaaggta atttaagtgt
    41641 atttggtgaa gaatcaagat ttagtatatt atacaatagt tttggatttg aaaataaaga
    41701 taaaaatata aaagaatcaa gtcatggaca aaatattact tttgaatata tagctaaaca
    41761 aaatccagaa gttatgttcg ttatagatag aggaatagct actggcagtg atgtaaaaga
    41821 aagctcaact gcaaaatctg ttttgaacaa tgatataata aaatctatgg atgcatataa
    41881 gaatgataat ataatatatt tagattcacc aacatggtat gtaaatgatg gtggtctaac
    41941 atctttaaat aaaatgatag atgatgcttc aaaagcggta aattaataat ccgatataac
    42001 caaaatgtat tttttataaa atgattatca aataaactag gagtttttaa gaactcctag
    42061 tttatttttt gcataaaaaa gatatagttt aaacaataat attatctata ataagataaa
    42121 tctattcaaa atctaataaa atatgaagtt atactagaca aaaagaat
//
LOCUS       DAEORZ010000017        41592 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEORZ010000017 DAEORZ010000000
VERSION     DAEORZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41592
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.017"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(574..825)
                     /locus_tag="KRH39_000819"
     CDS             complement(574..825)
                     /locus_tag="KRH39_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510742.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(1071..1226)
                     /locus_tag="KRH39_000820"
     CDS             complement(1071..1226)
                     /locus_tag="KRH39_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBG8510743.1"
                     /translation="MTLEVKIEVKIIKDVVILNITHWKKETSLLTVKIARSQGDLSYV
                     KYFANES"
     gene            1898..2122
                     /locus_tag="KRH39_000821"
     CDS             1898..2122
                     /locus_tag="KRH39_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8510744.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRD
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     gene            2808..3278
                     /locus_tag="KRH39_000822"
     CDS             2808..3278
                     /locus_tag="KRH39_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG8510745.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIY"
     gene            complement(3987..5141)
                     /gene="nagA"
                     /locus_tag="KRH39_000823"
     CDS             complement(3987..5141)
                     /gene="nagA"
                     /locus_tag="KRH39_000823"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG8510746.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            complement(5224..5949)
                     /locus_tag="KRH39_000824"
     CDS             complement(5224..5949)
                     /locus_tag="KRH39_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8510747.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            6216..7145
                     /gene="pfkB"
                     /locus_tag="KRH39_000825"
     CDS             6216..7145
                     /gene="pfkB"
                     /locus_tag="KRH39_000825"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG8510748.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLVEIDKYQDLVEVKKLS"
     gene            complement(7274..8191)
                     /locus_tag="KRH39_000826"
     CDS             complement(7274..8191)
                     /locus_tag="KRH39_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG8510749.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGNIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            8662..9831
                     /locus_tag="KRH39_000827"
     CDS             8662..9831
                     /locus_tag="KRH39_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG8510750.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            9880..10731
                     /locus_tag="KRH39_000828"
     CDS             9880..10731
                     /locus_tag="KRH39_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG8510751.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            11057..12958
                     /locus_tag="KRH39_000829"
     CDS             11057..12958
                     /locus_tag="KRH39_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8510752.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVAGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMVNKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            12961..14004
                     /gene="ypdE"
                     /locus_tag="KRH39_000830"
     CDS             12961..14004
                     /gene="ypdE"
                     /locus_tag="KRH39_000830"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG8510753.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKALAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            14019..14324
                     /locus_tag="KRH39_000831"
     CDS             14019..14324
                     /locus_tag="KRH39_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG8510754.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            14421..14756
                     /locus_tag="KRH39_000832"
     CDS             14421..14756
                     /locus_tag="KRH39_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8510755.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            14788..16110
                     /locus_tag="KRH39_000833"
     CDS             14788..16110
                     /locus_tag="KRH39_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8510756.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            16120..17190
                     /locus_tag="KRH39_000834"
     CDS             16120..17190
                     /locus_tag="KRH39_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG8510757.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            17195..18382
                     /locus_tag="KRH39_000835"
     CDS             17195..18382
                     /locus_tag="KRH39_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG8510758.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(18579..19451)
                     /locus_tag="KRH39_000836"
     CDS             complement(18579..19451)
                     /locus_tag="KRH39_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBG8510759.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNVLWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(19845..20036)
                     /locus_tag="KRH39_000837"
     CDS             complement(19845..20036)
                     /locus_tag="KRH39_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510760.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            20256..21302
                     /gene="cobT"
                     /locus_tag="KRH39_000838"
     CDS             20256..21302
                     /gene="cobT"
                     /locus_tag="KRH39_000838"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBG8510761.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            21334..21894
                     /gene="cobU"
                     /locus_tag="KRH39_000839"
     CDS             21334..21894
                     /gene="cobU"
                     /locus_tag="KRH39_000839"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBG8510762.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            21904..22677
                     /gene="cobS"
                     /locus_tag="KRH39_000840"
     CDS             21904..22677
                     /gene="cobS"
                     /locus_tag="KRH39_000840"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBG8510763.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            22697..23311
                     /locus_tag="KRH39_000841"
     CDS             22697..23311
                     /locus_tag="KRH39_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG8510764.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      23378..23557
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            23687..25186
                     /locus_tag="KRH39_000842"
     CDS             23687..25186
                     /locus_tag="KRH39_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBG8510765.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            25225..26601
                     /locus_tag="KRH39_000843"
     CDS             25225..26601
                     /locus_tag="KRH39_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBG8510766.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNK"
     gene            26602..27573
                     /locus_tag="KRH39_000844"
     CDS             26602..27573
                     /locus_tag="KRH39_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG8510767.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            27605..28675
                     /locus_tag="KRH39_000845"
     CDS             27605..28675
                     /locus_tag="KRH39_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG8510768.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTSLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNIQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYVL
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            28677..29564
                     /locus_tag="KRH39_000846"
     CDS             28677..29564
                     /locus_tag="KRH39_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG8510769.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKYMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            29552..30184
                     /locus_tag="KRH39_000847"
     CDS             29552..30184
                     /locus_tag="KRH39_000847"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBG8510770.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            30193..31419
                     /locus_tag="KRH39_000848"
     CDS             30193..31419
                     /locus_tag="KRH39_000848"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBG8510771.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDDGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            31416..32024
                     /locus_tag="KRH39_000849"
     CDS             31416..32024
                     /locus_tag="KRH39_000849"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBG8510772.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            32014..32589
                     /locus_tag="KRH39_000850"
     CDS             32014..32589
                     /locus_tag="KRH39_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBG8510773.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            32582..33334
                     /locus_tag="KRH39_000851"
     CDS             32582..33334
                     /locus_tag="KRH39_000851"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBG8510774.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            33318..34439
                     /gene="cbiG"
                     /locus_tag="KRH39_000852"
     CDS             33318..34439
                     /gene="cbiG"
                     /locus_tag="KRH39_000852"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBG8510775.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSIIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNAYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNTDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            34436..35161
                     /gene="cobJ"
                     /locus_tag="KRH39_000853"
     CDS             34436..35161
                     /gene="cobJ"
                     /locus_tag="KRH39_000853"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBG8510776.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            35158..35910
                     /gene="cobK"
                     /locus_tag="KRH39_000854"
     CDS             35158..35910
                     /gene="cobK"
                     /locus_tag="KRH39_000854"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBG8510777.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            35929..37407
                     /locus_tag="KRH39_000855"
     CDS             35929..37407
                     /locus_tag="KRH39_000855"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBG8510778.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFNGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            37437..38342
                     /gene="hemC"
                     /locus_tag="KRH39_000856"
     CDS             37437..38342
                     /gene="hemC"
                     /locus_tag="KRH39_000856"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBG8510779.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            38332..39831
                     /gene="cobA"
                     /locus_tag="KRH39_000857"
     CDS             38332..39831
                     /gene="cobA"
                     /locus_tag="KRH39_000857"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBG8510780.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELVPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            39848..40813
                     /gene="hemB"
                     /locus_tag="KRH39_000858"
     CDS             39848..40813
                     /gene="hemB"
                     /locus_tag="KRH39_000858"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBG8510781.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            40966..41544
                     /locus_tag="KRH39_000859"
     CDS             40966..41544
                     /locus_tag="KRH39_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBG8510782.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNVLVTLDLEELKKFDI"
BASE COUNT        16449 a         4460 c         7093 g        13590 t
ORIGIN      
        1 attattcatt tttaatagct ctttttagat gtcagaatta ttatattttg aaatttatta
       61 aatttttgca ttaaaaaaac tagaaattaa tttaagtctt gaattactta taactttcta
      121 cgtatctacc tttatattct tactctgatt ttatatctaa tatcaactct tcatcaaagc
      181 tattaaatat acctctaatt gagtcaatta tttcacttaa ttcaccttta atttttatat
      241 taacttcaac catttcaaat ctcatctttt tatcttataa tttctataag tttttttatt
      301 tattgtgttg cttactttga ccagcacttt tttatttaat agtctaaatg atatttttta
      361 ccagttttaa tatccttaaa tactatattt actaaaaact tataatttct tttaatacat
      421 agttaataca ttcttattac taataacaat actcaacaat tttatcgcct tttttcaaat
      481 atgatttaac ttttttattc ctaattattt aaaaatagtt gtccaatttt cttctatgag
      541 aaagtgctaa gtattagcaa cttctctatt ctcttaactt tctactgtct caactttctt
      601 catagctata ttaatagcta cttctttagc tacttcattg aactcttccc atcttctcct
      661 attttcttca tcagatatac gtggatatac tatataaact tctgtgtttt gattagctat
      721 aattttttca tcatattcta tatttttttc tttatcatat ttgtatggtt taaattgttg
      781 tagtattttc ataacatcac cccttgttat aaaatatgaa ctcataaact tgtcttatac
      841 ttatttaatt gttacaattt ttacttaaaa tcactttata ttctatattt taaagccaaa
      901 tcctttacaa aagtaacata ttcttctata agtttcttat cctcttatat aatatctagt
      961 ttgtttaact tatctctttt aaacttacat acgccttctt ctcccattcg tcttttatta
     1021 attgttaacc ttgtttcaag agacacacca aaactcttgt taagaagctc ttagctctca
     1081 ttagcaaaat attttacata acttaaatca ccttgacttc tagctatttt aactgttaat
     1141 agacttgttt cttttttcca atgtgttata tttaatataa caacatcttt tattatttta
     1201 acttctattt ttacctctaa tgtcatatta ttgcttgatt aatttgtatc cttaaatttt
     1261 ctattaatac atcttctata caggttagtt tctctcgctt aatcttgaaa tatcttttct
     1321 ctaaattgtc agattgttct caagttttat cagtatctaa tactttgtag tgtatatttg
     1381 aacatctttc ggtccaaagg tataattgac tagaatgttt tgaaatcaac tgactattaa
     1441 tagattagtt cttaaattct ttcaaatact cgaattgtaa taaaacataa tttacattct
     1501 ctttatttct attgaagtta gtttatatat tgtttacatc attttcatat atatctgcta
     1561 gttgctatgt atttaaaatt ctcttattat ttctttatat tacttgtaaa ttattcatcc
     1621 ttatcatcct cttaattaaa ttttctatat cataatcaag tatcttagat agtttatagg
     1681 taatatatca actctaattg aatattaccc tttcctgtac ttaaaataag ttgatgcatt
     1741 tttaaaacct aactcttttg caacactttg taagtataaa ttttatgtat gtcttctttt
     1801 ttataaaatc atagtgtatc tttttcatat attcacatta atttctatat ttcaataaca
     1861 atatttccaa agcagaaact tttcgagata attatgtatg gatgtaaatc aaaggattaa
     1921 atatattagg gaaaatatga atagttctca aaaagaattg cttgaaaaaa caaatataaa
     1981 tacaagtgtt atgaatagaa tagagtctgg tgaaagacca acaagagacg atgggttaat
     2041 aatatttgct aaaatttttg gtttatatac tgattatatt cttggtttat cagatacaga
     2101 aaaactaaat attggcaatt aaaactgaat taattgtatt atatgtgtga tgaatataaa
     2161 tagagaattt tttgaaaagc ttgaaataac ttagaaaaaa taagaggttc actcaagaac
     2221 aagttgctaa agattaaaaa acaacaaagg tatctactaa aagatatgaa aatagtttaa
     2281 gagagttgag tctggaaata tttatgtgta ttctttaaat aaaaatttat tttttaagtg
     2341 ataattaaat ttttacagac agggctatgg ttttaaaaat catagcctta ttttgatatt
     2401 ttttaaaaac acattgcaac ccagttgttc caaatattat atcatttact tttaaaatat
     2461 cgtaaactgc atactatata ttcattataa tataatttat ctaactagaa cctcagttta
     2521 ctaattaaat tagatgcata gaataacttt tttaaacatt gaaaataaat taaactctaa
     2581 acacatttaa atataaattt atatatcatt ttgacttggt taaacaattt catacttatc
     2641 gatataaaaa taggttataa tataaatgtt cattatctta taacctattt tatataattt
     2701 attcctaaaa catgtgaatg ttttttatga tagtttgaag aaattataaa atcactttat
     2761 aattgaatat tatatataaa ttaatttaaa tatagaaagg aaaatatatg tacaacaata
     2821 tcaaaaatat tttaaaagaa aatcatataa cgtttgaaga atatgaccat gaacctattt
     2881 tatcatttga aaaagctaaa gaaataagag aaaaattaca atatactgga gtagaaagta
     2941 aaagtttatt tataaaagat agatctggaa attactatat atttgtaacc acagaaggaa
     3001 aaaaattaga tccaaaaact ataaaaaatg ttttaggtgg aaaagtatct atatgttcaa
     3061 aagaagaatt aacacaaaaa acacaatgtt tgcctggatg tgtatctcct tttggatatg
     3121 agaaaaatat ctctcttgta atagatcaag atatatttaa ccatgataaa ataatctttt
     3181 caccagggat accagaaaag acatttatta ttacagctag tggatttaaa aaagtaataa
     3241 acaatataaa caatacaact tataaatata tatattaatg aattttaatc ataattgtaa
     3301 attagagaaa ataacattag aaatcatgct taactatagt gtaaataaat tatattcaac
     3361 acaaaagatt gatactatta ttacaatagt tactgttaag ctcaaattca atgtattatg
     3421 agttattatt tttaaatttt aattacaaaa gcagggtagt tttaactact ctgctttata
     3481 tttaacataa aatatataaa atcaacaaaa tgattttagt ctatctatta gatattcttc
     3541 gctctcaatg cttaaaactt ttagatatat acaataagtg ctttcatttg taaaaatttc
     3601 tatagtatta tcttgtttat ttacacctac aatatcttta aaattgatat gatttttagt
     3661 atttttacat ttgaaaatat atctgttatc aatcaaaatc tcatcattag taattgaaat
     3721 atagggttta tctcatgctt tattaaaaat ttatatgttt gctcaagata aaaacttaca
     3781 aataataata gaaaagttac tttaaatgta catattatcg aaaatattat cacaaaaata
     3841 cttataatat atatttgttt aaaataacct ctatcgcaat agtttattaa cgatatggaa
     3901 ataaaagtgt taaatcagat tttggagaaa taactttaaa tatttccaga ctctttttag
     3961 acagcctcca taaccttata ttgaacctat ttattaactt tgtacttaca gataccattt
     4021 atataagttt cgtgtatatt aaagtcatta tctgttacta taaagtctgc atccatacct
     4081 tgatatatag agccacagac attatcaata ttacaactta aagcagggac atatgtagcc
     4141 atttttattg catcttctaa agttgatatg ttccaatcaa ctatatttct cacagccttg
     4201 tccatagtta atacacttcc agctaaactc ccactattta atcttgcaac actatttttt
     4261 acatttacat taaacttacc aagagtatat tttccatcct ccatagcacc agctctcata
     4321 caatctgtaa ttaaagctat gttattacta gacttcgcat ccatcattat tttaacagct
     4381 attgggctta catgatgacc atcacaaatt aattcagcat aagtatcttt tagtaccata
     4441 gcagctccaa ccattccagg ttctctatga tttaaaggac tcatcccatt atatgtatgt
     4501 acaaacactt tagcacctga attaacgact tgtttaactt gctcaaatgt agctgaacta
     4561 tgaccaagag aaactgatat attattttta gttgcatatc ttgttaattc tattgaacct
     4621 ttacgttctg gagcaattgc tatttttctt attaaatttt tcgatttttt ttgccataat
     4681 tttaatatat tgatgtcagg gtctttaaaa tatttttcat tttgagcgcc cttatatttt
     4741 tctgtaaaaa atggaccttc taaataaatc ccttgtattt ttgcaccctc aactcgattg
     4801 aagctattgc ttatattttc tacagaatca cttagtgtat ctacagaatc agttaaagtt
     4861 gtaggtagga aagatgttac tccatatttt aataagcctt ttgaaattgt gtttattgct
     4921 tcaaatgtat tgtccatgac atctgcacct aaaagaccgt gaatgtgagt atcaactaag
     4981 ccaggagcaa tatatttacc tgaaaaatct ataacattat cagtaacatt aggtctttct
     5041 tgttgaaatt caccgaattt gccattaatt atactaaggt aacctttttc tttgattcca
     5101 tgtttcataa aaaatttatc tgcataaata aaacttttca aaataacctc cttataatta
     5161 tactatcatt gtggcattga cgcttataat atcattctat tttcttgaaa gctatttaac
     5221 ttactatctt acatgtctta ctctatatac aaatttatcg cttctagcga cactcaaagt
     5281 aaattcaata actatattgt catcattata tgtggtacga agtaatttca gacaaggtga
     5341 atctcctggt acttccaata acttagcttc cttatcagag attattccag ctgaaaattc
     5401 ttcatcagca actttaataa tctcgccata atcttctcta aatatttcat ataaaggttt
     5461 ttgttcgatt ttttcttttg tcaatcctat aaattctttc ataggaagat atgttctttc
     5521 aagcatcata ggaaggtcat cagcaagtct taaacgcatt atttttatca ttttttcacc
     5581 aggagttata cctaattttt cagagaaata ctttgtactt tctactacct caaatgataa
     5641 tacctttgta cctggctttt ttccttgtgt tctcatataa tctgtaaagc tataactttc
     5701 cattaagttt tgagaatttt gacttagtgc agcaacaaaa gttccttttc catgtctttt
     5761 gtatatatat cccatatgct ctaactcagc catagctagt ctaactgtgg ttctacttac
     5821 tccatattca tcacaaattt ttctttcgga aagcattcta tcgtttggtt ccatctcatt
     5881 ttcaatctta gttcttaata tctcaacaag ctggtcatat attggtcttt tatcatttct
     5941 ttttatcata aaaatatagt cctttctaag tggttattac cacaatttga ttatagaata
     6001 tagaattatt tatgtcaatg aataaagttt aatcaaaatt caataagtaa ggctattaat
     6061 ttattataaa attatggtac attgacaaaa caagtaaaat aatttatatt caaattggta
     6121 ataaccactt tggatatatt atatgaggta ttttttattt tacagattaa tttattgttt
     6181 agaaaagtaa aaattaataa tgaggagaat ctagtatgat tacagtaata acattcaacc
     6241 catctataga tagaatgtat agagtgaata atataaacat aggtgaggtg caaagggtag
     6301 taagtaagaa tgcaactgct ggaggtaaag gattaaacgt agctaaggta tgtaaaattt
     6361 taaaagaaaa tcctttagct atgggatttc taggcggatt taatggagaa tttataaaaa
     6421 atgaacttag aaaactagat atacaaaata aatttacaaa aatagagcag gaaactagaa
     6481 attgcttaaa cataattgag gacaataaag ttagtacaga atttttagaa aaaggaccaa
     6541 tagtagaaaa aagcgattta gagagatttg aaaatgatat agaggaagta ctaaaaaata
     6601 ctaaaatact ggtagcttct ggtagttatt gtgagaatat gccgatagac tattatgaaa
     6661 agataggaaa tatatgtaga gcaaataata taaagtttat attagataca tcaggagaag
     6721 cattaaaaat agctttaaaa tcaaaaccat atttaataaa gccaaatata gatgaaataa
     6781 gacatttatt aggaatgaat atagaatcaa gagaagaaat tatcttgtca ggaaaaaaac
     6841 ttattgaaat gggtgcagag aatgtatgta tatctttagg aaaagatggt atggtgtatt
     6901 taaacgaaga taatgtatat gatgtaaaag taccagttgt aaagtgtata aatactgtag
     6961 gtagtggaga tagcacagtg gctggattta gtgtaggtat tttaaggggt tataaaatag
     7021 aaaatttact taaatttgca aatgcttgtg gtataagtaa tgctttaaat atggaaactg
     7081 gatttgtaga tttggtagaa attgataaat atcaagattt agttgaagta aaaaagttaa
     7141 gttaaataca tttaatggga atataataaa tttaatgttg ttgtaataga ttttaatttt
     7201 aatataaaag atatagtctt atataaaaat aggttagaaa aattcatttt taacctattt
     7261 ttataataaa tatctatttt actgaattta taattttctc tagtaatctt tgagccttga
     7321 tacactcttc tccattaata gctggtttta tattattttc tatacaatca ataaaatgat
     7381 ttgatatatc ctcaaaaccc ttttgtttta atatgtttac ccatgcacca ctttgcttta
     7441 aagttaggtt tccaccttct tctatttcca gtacattaag attcttaact ctttgaatat
     7501 taccctttga aagaatttca acttgttcta actttgttcc agaatctcta tgcatagagg
     7561 tgtttataga gaaattttta cctttaagtt tatgacttac aaatataaga ttcttattgt
     7621 ctgttaaaaa taaatcctct ccactaattt caacatcttc atttgctaac cataaagcag
     7681 tgtcaataac atgtatatag tcatctataa gagtactgtc gaatttaaca tttcttaagg
     7741 aatttaatcc atgtttacat atattgatag atacaatttc tgtagcatta tttttaattt
     7801 ctttgtacat aggacagaat ctacggttga atccaaccat taaatttaag ttttttttcg
     7861 tacttaattc gataagttct tctccttgac ttactgttga agcaagaggt ttgtctacat
     7921 atacatgaac acctaaattt aataatattt ttataatttc gtagtgtgtt tctgtagagc
     7981 tatgtagaaa aatacaatca cattttttag ctagggattc aatagaatcg aatggcatga
     8041 ttctataatc actacagact ttttcccttt taactttatt tggagtaaaa gctcctacaa
     8101 actcaaatct ctcagactta gttaatatag gtaaatatgc cttttgagct atggagccaa
     8161 gacctatcat tcccattttt atatttttca taatactttc tcctaaggtt tttatattta
     8221 cagaattgat tataattcag acacataaat ttgtaaactt tgtatcaaaa aataaattta
     8281 aagcttatat aagtaaatta gcgaaataag aaatattgat aaagtaattt tgtaggatat
     8341 ccataaaatt gattgatata aatattttat gtattgacaa aaaattcaaa ttaatttata
     8401 tttggattag tggtaattac cactttggtc tgaacaagtt tttatcgtaa aataaaaata
     8461 ctaaagtata tagagtatga ttttattaaa tttttaaaga taaactataa aattaattaa
     8521 aatctatagt gtatatttta tagtgagttt aagtagtact aaaaaatcta agcaaaaaat
     8581 tataaaagag tatatttttt tatagtttaa agtggttata acctcattac caaatgtaaa
     8641 cgttacaagt atgaaaggag aatgaaaatg tttaaattag aagaacaaaa actaaaagat
     8701 ttaggtgcaa taataacaac taatgaaata aaacaacagc ctgaattatg gttagagact
     8761 tatgaaatat ataaatctaa caaagaaaaa ttaagtagat tcatagatac aataagtaat
     8821 aatcatgggc agtttagagt tatatttaca ggtgctggaa cttcagctta cataggaaat
     8881 tcaatacttc cttaccttaa gaataaaaat gacataagaa aatacatatt tgaagcaata
     8941 cctactacgg atatagtttc aaacccatat gattacttaa aaaaagatat accaacatta
     9001 ttaatttcat ttgcaagaag tggaaatagt cctgaaagtt tggctgcttt aaatttaggg
     9061 aataagattg tagataattt ttatcattta gctataactt gtaatccaga aggagaacta
     9121 gctaagatga ctaaaaacga cgaaaataat tacttgttat taatgccatc aaaatcaaat
     9181 gatgaaggct ttgctatgac aggaagtttt tcgtgtatga tgttatctgc tatgttaata
     9241 ttcgatagtt tagaagatga tgtagaaaag tcatatataa atgcaataat tgaaatggga
     9301 agaaatgtta tagatagaaa agatgaaata catgagttaa taaataaaga ttttgataga
     9361 gttgtatatt taggttcagg aggattaggt ggtttgacac aagaagcaca actaaaactt
     9421 ttagaactta cagcaggaaa aatatctaca gtgtatgatt ctcctatggg gtttagacac
     9481 ggaccaaaat catttattga tgaaaataca cttgtatttg aatttgtatc taactgttta
     9541 tatacaagaa aatatgattt agatgtttta gaggagataa aaagggataa aattgctaaa
     9601 ttcacttgtg cagtgtcagt agaaaatgaa aataacttct caggaacaaa gtttgagttt
     9661 aaagaaaagt ataataaatt accagatgtg tatcttgcaa tgccatacat attgtttgca
     9721 caaactatag cattgtttgt atcggtaaag gttggaaata agccagatac tccatcagct
     9781 acaggaacag taaatcgagt agtaaaaggt gtaacaatat atgaatatta aaagataaga
     9841 ttaaaatatt ataactaaat aatttgggag ggtatcaatg tggcattagt aacaacaaca
     9901 tcaatgctta aaaaggcaca agatgaaggg tatgcagtag gagcatttaa tgtagaaaat
     9961 atggaaatgg taattgctgt tatagaagct gctgaggagt taaattctcc agtaatatta
    10021 caaactactc catctactgt aaaatatgca ggtcttgatt attatctagc aaatgcaaag
    10081 gttgctgcag aaagagtaaa tatacctgta gcaatgcatc tggaccatgg ttcaagtttt
    10141 gaccttgcaa tgaaagcttt aagaagtgga tatacatcaa taatgataga tggttctcat
    10201 gaaagctttg aaaataatat atctatatct aaatcagtag taaaagcatg tagtccttct
    10261 cttataccag tggaagcaga acttgggaaa gttggtggaa aagaagatga tttagatggc
    10321 gggaatagta atgattatac aaatccaaaa gaggcaaaag agtttgtcga tagaacagga
    10381 atatcttcat tggctattgc tataggtact gcacatggat tgtataaggg tgaacctaaa
    10441 atagatttag ataggttatc agaaattaaa aatgtagttt caataccttt agtattgcat
    10501 ggaggttcag gaatacatga tgatattata aaagaagcta taaaaagagg tattgcaaaa
    10561 gttaactatg caactgaatt aagaattgca tatagtaaag gggttaaaaa agttttagat
    10621 gaagattctg aggttataga ccctaaaaag tatggattag cagggcttca atcagtaaag
    10681 gattttgtaa aagaaaagat taaagtgtgt gggtctgtaa atagagtata aaaataaaat
    10741 atcctaaatt tattttagat aagtttagga tatttgttat atgaaaaatt tatattaatt
    10801 atatgtaaat ggtgtatttt tttcatttaa ttgatgaaaa atatcagctt ttattggtaa
    10861 agtattacat atataattaa aatatactaa aaattgaact atatatttta ttattataaa
    10921 atatttatat taaattgtgg aattaaaata tactaggata taaaattctt aaataaaaag
    10981 aaaaggttgt ttttataaaa aatatttttt ataccacttt aaaaaacaca ataataacac
    11041 tgagggaggt ggggatatga gagaacgcat tattcttgaa acattaaaaa atagtagtgg
    11101 ttatctaaac atagattatt ttgcagataa gttgggggta tctactcgaa ctatcagaaa
    11161 tgaaataaaa aagatagaaa ctataaaaga atgtaatggt tttaaattag aatataagtc
    11221 taaattagga tatattctaa atattaaaga ccaagataaa ttcgagcatt atctaagaaa
    11281 cttaccctca gatttagttg aaaatccaga acaacggttg gagtctataa tagtagagct
    11341 tttagtaaat gaaggttata aaactataga acagttaagt aaaaaatttc tagtaagttc
    11401 atcccaaata aaaaatgatt taaaaaagat agatgaaaag ataaaggata cagaattaaa
    11461 actagagcga aaggctcatt atggaattaa aatagaaggt tctataaaag aaattcaaag
    11521 tatattggtt gatagttatt ttagagggaa tagaaatata acagagtaca ggaataagtt
    11581 tatagataat ttaaaattag ctaatattag aagtacaata aaaaatgtac ttaatgaaca
    11641 tgatttagaa gcaaatttaa cagaacttga ggaaatatta gcacaaatta ttatattgta
    11701 tataagagtt agtatgagag tagcgggaaa tgctagcgat ttaaaattat atacagaaga
    11761 tttaattata gataaattat tagataaaat ttttagagat aaaaaatata accttaatta
    11821 tgatgaaaag tattatttaa aggagcttat aaaactaaag actaaagata agaaagccac
    11881 tataaagaat attgataaga ataaacttca agatattatg ttcgaatttt ttagagagat
    11941 agataaaaaa tataactcta attttttaga agataaagag ttctttaact tattttactt
    12001 acatattgca tgtttaatag aacgtataaa gaagaaccac aagataataa atcctttttc
    12061 agttaaaata agtcaacagt atccaactgt ttttaattta tctattcaat tttcaaaaat
    12121 catagaaaat gagtatcata taaaaataag tcaagatgaa atagggttta tagctactca
    12181 cattgctgtt ccttttgaaa agagagaaga agctaatttt aataaaaaat ataaaatagc
    12241 aataatatgc tcttctggtg gaggtagtgc atttttgata aatttaagac ttaaagaaat
    12301 ttttccaaat gctgagataa gaaatttttc attactagaa gaaaaagctg taatagaatt
    12361 tgcaccagat ttgatttttt caataacaaa tcttttattt gaaataaatg ctccagttat
    12421 acttataaat gaaattttag acgaattgga ttatttagat attaaagaaa gtgttagatt
    12481 tgcagaccat ataggaaata tatcaagccc aaaacaatat atattaggtc tatttgataa
    12541 gaatcacttt aggtgtataa aagataaagc taagtataag gatatccttg atagtatgtc
    12601 aaaatgtata gttgacgagg gagcttgttc tccaacctat cccaaagatg tatgggaaag
    12661 agaaagctat ttgagtacta tttatacaaa tggggtggct atacctcatc caatcgagat
    12721 gacaggaaac aaaaatataa tatcagtagc tttgatacaa actgatatta tttatgaaaa
    12781 tagggtgcct aaaattattt tcatgatttc attaataaag ggaaatttag aacttcataa
    12841 gcagatatct aaatatttaa gtaagattat ggtaaataag gatatggtag acatgctaaa
    12901 taagtcacaa tcttatgaag agtttatgta taagttaaaa atctatctgg gaggttagac
    12961 atgaatatag agttttttaa gaggttgtca gaagcagatg gtatagcatc taatgaagaa
    13021 gaggttagac atgttttatt ggaagaacta aaagcttata gtgataaaat tatctgtgat
    13081 gggctaggga gtatcatctt ttctaaaata aaagatgaga gtgcaccaaa cgtaatgatt
    13141 tgtgctcata tggatgaagt tggatttatg gtgagaagta tagacaaact tggtatgatt
    13201 cacttaataa caataggagg agtaaaagct cttgcacagt ttgtacaaaa agttagaatt
    13261 acaacaaaag aagggaaaaa aatccccgct gtcataaatg ctacatataa taatggtaag
    13321 gctgaaaata tatatgcaga tattggagca tatacagaag aagatgtata taacctaggg
    13381 attaatgttg gggatatggt aacatataca acaagctttg aggaatttac tttaccagac
    13441 cgattagtgg gaaaggcttt tgatgatagg ataggatgtt ttgtgatggg agaagtttta
    13501 aaagaactta ggaaagaaaa tctaaattgt aatattcact ttgcggctac aagtagtgaa
    13561 gaggttggaa taaggggagc aaagacatca acacagttaa taaatccaga tatagtattt
    13621 gtaatagatg tagcatgtgc taaaaatgag tttgtgagag actatacaaa tcagaaacaa
    13681 attgataaag gtgtaatgct aatgcataga gataggactc tagtccctag tagaaaaatg
    13741 atagattatg taatggaaat tgctgataaa aataatatac cacttcaaca tgatatgttt
    13801 gaatcaggtg ggactgatgg aggagaagca catctcgtaa atgaagggaa accttgtgta
    13861 gtaacttgtg taccagttag atatggtcat tgtgcatttt caatagtaag taataaagat
    13921 ttagagaaca ttataagatt gtatacacag ttaattttaa attttgatga agaacaatat
    13981 aaacatataa gaaattttat ctaaaggaga ataagaaaat gtcaacagaa gtacaaataa
    14041 tggaacttat atcaactgca ggagagagta aatcgaaagc ttttgaggct ttaaaaaaag
    14101 tgaagtcaaa agattttaaa ggagcaaaat tgttaataga ggaggctcgt aaaatagata
    14161 ttgaagctca caggatacaa acagaattaa taactcaaga tttaaatgaa gatgttgaaa
    14221 gtccagcaat gagtctttta atggtacatg ctcaagacca ctatatgact tctcagctag
    14281 caaaagactt gatagaggct ttgatatatt gtctcgataa gtaattgtaa tgtagaaata
    14341 gattatttat gtaaaaattc ttttaaaagt ttagatgctt ttaagagata ataaaaatac
    14401 aaactttagg aggaattatt atgaaaatat tattatgttg tgcaggtgga ttttctacta
    14461 atatgttaat gcaaaatatg aaaaaggtgg taaaagagag tgctaaactc aatgaaaaag
    14521 attttgagtt tacagctata ccagcagatt caatagaaag tgaagttgat gattgggata
    14581 tagtacttat aggtcctcaa atagctcaca aaatagactt cttaaaagga atattagagc
    14641 caaaaaataa gccttttgca gtaatagata aagatgtata tggaaaaatg gatggagcta
    14701 cagttatgaa gttggcatta ataacttata agaaacatca attagctcaa ggataataaa
    14761 aataatgagg gaaaagggag attagatatg ttttcaaaat ttgaagcatt tatgaataga
    14821 gtatttatgc ctttagctca taaggttgac aatcaacgtc accttggttc tataaaggct
    14881 ggtatggttg ctatgacacc attcacaata ttaggaagta tatttgcaat acttccagca
    14941 cttccaaata tgcttggaga aaataatcca ataagcaaat tcatacttaa caattcagca
    15001 atattggatt taccagtaag tctttccata ggtttaatag gattatatgc ttgtatgtgt
    15061 attgctttta atctggggaa ctactataaa ttatatatac caggttgtgt tgcactatca
    15121 actttttctt ttttattcct agtagcctca tttacaccag atggagcttt agaaatagga
    15181 aatttaggag ctaaagggct atttacaggt atgatagcag ggcttggttc tgtggagtta
    15241 tataattttt gtaataagaa gaatatcaca ataaaaatgc cagaaaatgt acctgatttt
    15301 gtatctaggt cttttgaatt aatacctact actataatag tttgtggaac ttttatagtt
    15361 gctcgtttta tttcattaaa agtatttgga gaattacctc cacagatact tactcaattt
    15421 ttatctccat tagtaggaag tatggataac ccatggtcag ttcttgcact taactttgct
    15481 atatgtacta tattcttttt tggaatacat tcatcagtat ttagtcctat aactagacct
    15541 ataatgatta catttatagc tgaaaacata gcagctatgc aagctggaga aacaatacct
    15601 catttcttta cagcaggagc ttcaagtgca ttctttggat ttacaggatg tggaattagt
    15661 ataggttgtg taattgcatg tatgttatca aaaaatgctt cctataagaa gattggacgt
    15721 gtatcattat tccctgcatt atttgggata aatgaaccaa tattatttgg agcaccgata
    15781 atattaaatc caataatgtt tataccacat gtatttggtg gagctataat aggtacacta
    15841 ccaatgtttt ttatgcactg gggatggtta gcaaagccag tatttgaccc tccatatgta
    15901 ggggtattct tagaaggatt tttaactaat ggagattggc attctatatt agcaaatgca
    15961 ttgcaattag tattagcagt tttattatat tggccattct ttaaaataat ggaacgtcaa
    16021 caaaataata aaaaacaaaa agaagaaact aagaatatat tctctaaaga agaagaagac
    16081 ttattggatg atttaggatt ggatttctaa tattttgtta tgaataatac aatggagaga
    16141 ttagtatcct taatctataa aagaggtgtt gacgctcttt tgattaaagc aaaaaatact
    16201 aaaagatata taggagcttt atcaggtagt ggtgtatatg tgcttgttac taaggataaa
    16261 aattatcaaa ttctagatgg cagatatatt gatgaagcag ataaaaaaac gactggtttc
    16321 ataaaaagga ttgtatctca aggttcttat ataccaacta taattgagtt attaaaagaa
    16381 ttaaatataa aaaagttagc aatagaaaac caagctatga gtatacaaga gtatctttca
    16441 ctaaaaaatg aaggttttga aatcaattta ataacaaatg aattatggaa gctaagggct
    16501 ataaaatcta aagaagaaat tgaacttatt aaaaaggctt gtgaaattac agatgaagta
    16561 tttgatgagg ttatttctga aattaaaaaa gatatgactg agctagaagt atcagctcta
    16621 attcagtatc atgcattaaa aaaaggtgca agtggaatgt cttttgaaac aatagtggta
    16681 tcaggagaaa gaggtgcaat gccacatgga agacctacaa acaaaaaatt gaaagtgaat
    16741 gaagctataa caatagattt tggtgttgtt tatcaagggt atcaatcaga catgactcgt
    16801 acaatatcta taggtaaacc tcctaaaatt attaaagaga tatatgatgt agtattggaa
    16861 gcacaattat ctgcaataga gtcaataaag gaaggaacta gggcaagtga tgttgacaaa
    16921 gtggctagaa aaattataga taaacatgga tttggcgaat attttaatca tggactgggt
    16981 catggtatag gtcttggaga tggtgaagtt cccactttaa atccaaatag tgaagatata
    17041 ttagtggaag gaatggtaat gtcttgtgag ccaggtattt atattccaaa tgtaggggga
    17101 gttagaatag aagatgatat tgttataata gatggaaaag gtgttccact caataagaca
    17161 tccaaagagt ttataatatt aggagagtaa aattatgaaa tataatttta ataaagtatt
    17221 aaatcgtaaa gaaggaaatt gtagaaaatg gagcaaccat gtgattaaag aaaaatttgg
    17281 cttaaatgaa gatgcaattc ctatggattt agctgatatc gattttgagt gtgctcctgc
    17341 tataaaagaa tctatcttaa aaagagcatc agtaggagat tatagttata cttttatagg
    17401 agatgacttc tatgatgcta ttataaattg gaataaaaga agatttgatg tatatataga
    17461 aaaggattgg ataaaattaa cttttggtac tgtttcaacc cttcattata tagtccaagc
    17521 atatactaaa gaaggagagg gtgtattgat aaatacacct gcttatgacc cttttgcaga
    17581 ggctgtagta aataataata gaaagttgtg ttgtagctca ttaaaattag ataacaatag
    17641 atattattta gatttagagg atatggaaaa tcaaatgaaa tacgagaata taagattgct
    17701 tatattctgt agtccacaaa atccatcagg tcgtatatgg acaaaagaag aactatatca
    17761 agtgtcagag ttatgtttaa agtataatgt aattttggta agtgatgaaa ttcatagaga
    17821 tataatattt aaagattata gatttgtatc tttgtggaat gctcatccag aaatttataa
    17881 aaatagtata atctgtgtat ctcctaataa aggcttcaac ttaggtggat taaaaacatc
    17941 atatgtatta ataagagatg taaaaattag ggagacttta ttagaaaggc taaaaagtaa
    18001 ttcaataact tctccaaatg tatttgcaat accagcaata gttgcagcat ataatgaaag
    18061 cgaagaatgg ctagatgcta tgactagtta tgtagaggag aattttgaaa tagtttatga
    18121 tttctttgaa actaatatac caaaagctaa agtgatgaag tcagactctt cttttttagc
    18181 ctggatagat gtaagagaag tatttagaaa tgaagaagaa tctaaagaat tttttagaca
    18241 tgcaaactta actatggtag ttggaagtta ttttgtgaaa gatggagatg gatttattcg
    18301 tattaatgtg gggtgtccta gagaaacatt aaatgaggct ctaaatagaa tcaaaaaaac
    18361 gtatataagt atgtattgtt aaatcataaa aaaagtatct atggtttata taaaaacaca
    18421 ttatttagtt atcaaatatt actggaattt tgtattccag taatattttt tgattaagga
    18481 atattattga ggaatattaa ataatattat ttcataccct tatattaact tatataaaaa
    18541 tattttatta ttttgggaac gggttagcta acctttatct agtgtatatt tatattgtaa
    18601 gaagcagtaa aaacttttcc tttactaagt ttattaataa acttcttatc tttataaatt
    18661 ttgttagaat ttatactatc agcaagacca taccaaggtt ctatacataa aaaaggagca
    18721 gtagaattag tttcactata ataaggagtc caaataccaa ctagagggaa atcttccata
    18781 gatatagtta catatttact accatcatta ttacatatag acacttcatc tatgttggtg
    18841 tatatcaaag catcattctt gaatacttct ggatttaact gtaagttttt taattttcca
    18901 ataggtatta tatcatctgt aaaagaacca tttaaattga ttttttctac atcattttta
    18961 cttttaaact caaggtagtg tttagaaaaa tcattttgtt cgtaaaaagg aaggttgaat
    19021 gcaggatgtc cacctataga aaagaatata tctttagaat cagtattttt tacaatccat
    19081 tctacctcaa tactattatc atttaatgta tagtttatga ttagctcaaa agaatatgga
    19141 tacttcttta aagtagattc attatctgtt aatttataag ttacaaatgt atctccttta
    19201 tctacaatct caaagtccat atctctagca aacccatgtt gagtcatgct atataaattt
    19261 tcttctatta ttgtttcatt atcaaaaagt ttacctacta tagggaacaa tataggtgat
    19321 tgtctacccc aatatttttt attaccatcc caaagaacgt ttttgtcgta ttttttagaa
    19381 aatattttag ttagttcagc accaaaactg cttgattcga taattaaatt ttcattttgt
    19441 aatatattca tttaaaatct cctttattat attttatgag tatattatac tataaagtat
    19501 aaagattatt aaattttatt tgatattata tttgaaaggt agattttagt atattttgaa
    19561 aattttgttt tgaaaatttg ggattattta agaaattttg tattataaat agaaggaaat
    19621 agataaattg tatagaataa aatatattaa gataagttaa taatgttaaa tgggaagtga
    19681 gtttatggaa aaaccaaata caaaagttgt taactttcaa gagtataaag acagaaaaaa
    19741 ccaaagagaa gtaaatagag acgaactttt aaagcttata aaaaaaattg tgacttcaaa
    19801 atagggagtt gttaaactcc ctattttaat gggtttcata ataattaaga cattctaagt
    19861 ccatctatat aatctttgaa ttgtgagaaa tctgattcac tacttaattc tatggtttct
    19921 gaaccttccg tataaaaatt cttaagatag atagtataat tattatcaac acaatcatga
    19981 taaatacatc tatatccatc ttcatacaaa ctttttaatt tagaaaaatc atccacaata
    20041 tcatctcctt aaagttcttt taaatatatt tttacaaaaa gcatctaatt gtatgcaaat
    20101 tgacaatgta aaataaagga aatgaacata aaatgtagaa taatttttat atgtatgtaa
    20161 aaataaaaag aaaaagttga aatttttaga aaataagagt attttttatc taaaaagaga
    20221 gtttaaaaaa taaaaaaatg ttgggaggat aaaaaatgag tttattagaa agtatatcaa
    20281 aaaacatata ttccctagat aactcttcta ttgaaaaaac aaagcagaga ttggataggc
    20341 ttatacatcc aactggaagc ttaggaaaga tagaagatat ttgcatgcaa ttagcaggaa
    20401 tatttggcaa tgaaaatttt gacacaagta aaaaggttat aattgctttt gcgggagacc
    20461 atggtgtata tgaagaaggc gtagcacctg accctcaaaa tataacaaaa ttacagtttc
    20521 caaatttttc gaaaggtcta tgtggagttg gagtaataag taaatttgta ggggcagatg
    20581 ttgtagctgt agatgtagga ataaactgtg atgaaaaact ggatggggta ttagactaca
    20641 agataagaaa agggacatca aatatggcaa aaggtccagc tatgagtaaa caagaagcaa
    20701 ttagatgttt agagataggt attgaaatag cagagcagtg tatagaaaaa gattataagg
    20761 ttattggtat tggggagatg ggtatagcaa atactactcc aagtacagca ataatatctg
    20821 taatttcagg gtgtgacccg ttagaagtta ctggtatagg tgcaggtctt aagaaagaaa
    20881 gactaaagca taaggctgaa gtaattagaa aggctattga aattaataat ccaaatccaa
    20941 ctgatggagt agatatttta tctaaagtag gaggatttga aataggctca atggcaggtg
    21001 ttatattagg ttgtagtgct aataggattc cagtagtaat tgatgggttt atttcttatg
    21061 cagcagcatt aattgcatat aagataaatc caaaaactag agaatatatg atagcatctc
    21121 atttatcagc agaatcagga actaaaagag cattagacat attaaaatta gaccctctat
    21181 taaatatgga tatgagatta ggtgagggaa gtggagctgc tttagcattt gatataatag
    21241 aagcttcaaa ctatacttat aaaaatatgg caacttttga tgaaattgac atgggaagat
    21301 aaatataaaa atttaatatg taggagtgtt ttgatgagta aaattattct agtaacagga
    21361 ggagctaggt cagggaagag taactttgca gaaaaactat gttttgacag aaataataat
    21421 acagcttata tagcaacctc tattccattt gatgatgaaa tgaaagatag agtaaaaaaa
    21481 catcaagaga atagacctag aaactggaat acttatgaaa tatatgaaga tatatattcc
    21541 ataattaagg agatatataa agaacatgaa actgttatac tagattgtgt aacattatta
    21601 gtaaataatc taatgtttga acacaattta aatatagaag aatctagtca agaagaaata
    21661 aacaaattag aacaatacat aaaagaacaa gttgtcaaac ttattgaaga aatagaaaaa
    21721 acaaatctat actttgtatt tgtaacgaat gagttaggta tgggaattgt tcctgggaat
    21781 aaacttagta gaatttatgc tgatattgta ggtcgtataa atcaatatat agcttcgaaa
    21841 agtaatgaag tttattttgt agttagtggc atagctatga agataaagga gtagtatgca
    21901 attatgaaaa gatttatttt aatactacag tttttgacaa ggatacctat aaaattaaat
    21961 gtaggatttg atgatgaatt ttataagtca atagtatatt ttccacttgt aggttttgtc
    22021 ataggtatat tatcctattt aataggatgg atttctatgc ttttatttga accatttata
    22081 gcttctataa taataactct agctggtgtc ttaattactg gaggacttca tatagatgga
    22141 ttaggagata catttgatgc aatatatagt tatagagata aagaaaaaat gcttgaaata
    22201 atgaaagatt caagattagg tacaaattca cttctagcaa ttatgtttgt acttttatta
    22261 aaggttggat tcgtttacga tataataagt aataattcat tgtgggttat aatttttatg
    22321 ccaatgattg caaggttagg agtaatgtta ttgacatata aaactgtgac accaagagaa
    22381 aaaggaatgg gaaatttatt tataggaaaa ttaactacaa gtatgttaat aactgctata
    22441 atatacacat tattgatagt tgctcttatt actaaattta tatttttatt acctaatata
    22501 gtgttgatta aagtattagg ttcgattata gtggtttttg tatttataat attattcaaa
    22561 aaacatattt ataaaaaaat tgatggagta acaggagata tattaggctg tggaatagaa
    22621 ctttcagagc ttgtatattt aatttacata tatttgttaa tttttatgtt tttttaaagt
    22681 aaaaagtggt gataatatga taagattaat attaataaga catgcactga caaatgataa
    22741 taaaaaagga agattatcag gccatataaa tagttgtatt agtgaagagg gcaaactaca
    22801 aataaataaa attaccagat atctgagtaa tgaaaacata gataaggttt atacaacacc
    22861 atctacacgt actaaagata cagtagaaaa aatatcaaaa ttgaaattat tagaaataga
    22921 agaaaaagaa gctttaagag aaattagttt tggagacttt gaaggaagaa cttttgaaga
    22981 aattaaaatc aaatatccaa atgagtttga aaaaatgata aaagaaggta acaactatag
    23041 atatccaaat ggagaaagtt tgatagactc ttataaaaga gtggcaaaag aaattgataa
    23101 tataatttta gaaaatgata gtaatttaga tacaaagaca atacttatat gttctcatgc
    23161 aggaacaata agaaatataa taacacattt gatttctggt agttataagt atcactggaa
    23221 ctttaaaatt gataatgcat ctattacagt tttagaaatt gatggtggat ttgctgtaat
    23281 agataaaatg aattttacag acttcatata atttaatcaa aaccaaacat tacaatctgt
    23341 gtttgtaata tatctgtaaa attagcaatg aaaatagaat attagattaa aattaggttc
    23401 ttaattagat gttaaaaggg aaaaaggtta gatgcctttg cagcccccgc tactgtgaaa
    23461 ccaacgaaac tataaatacc actgtcagtt tgatgggaag gttatagaag taggatgaag
    23521 ttaagccagg atacctgcct aatttaattt aaaacaagag gtcttcgggg tgaaagattt
    23581 ttaattaata tatataaaag tcctatcttt agaagtatta ggactttttt taatttgttt
    23641 tttagtaaat tttaacaagg agggatggaa agtggggaaa aaaataatgc tacaaggaac
    23701 agcttcaaat gtaggtaaaa gtgtcttagt agcaggtttg tgtagaatat ttaagcaaga
    23761 tggttatact gttgctcctt ttaagtcaca aaatatggct ttaaattctt ttataactaa
    23821 agaaggatta gaaatgggaa gagcacaagt atttcaagca gaggctgcga aaatagagcc
    23881 aatagctgat atgaatccag ttttattaaa accttctgga aataataaaa gtcaagttat
    23941 tgtaagaggt aaagttgtag gagaaatgcc ttcttctaag tatcatgatt ataaattgga
    24001 gttagtagat gtgttgaagg aaacatttaa tagcttaaat tcaaggtatg atattgtagt
    24061 tatggaaggg gcaggaagta atgcagagat aaacttaaaa gatagagata tctctaatat
    24121 gggtatggca gaaatagctg atgcacctgt aataatagtc ggagatattg atagaggagg
    24181 cgtatttgcc tcattagcag gaacaatgct tttattaaat gaagatgaga aaagaagagt
    24241 caaaggtgta ataatcaata aatttagagg aaaaaaagag cttttacaag atggtgtaaa
    24301 gatgctagaa gacatcataa aagtacctgt tataggagta gtaccttata cagatataaa
    24361 aatagaagat gaagacagtg taactacgag atttaagaaa aaaatgaata aaagtgatat
    24421 acacatagaa ataattagaa ccccacatat gtcaaatttt acagatttta atatatttga
    24481 gacacaagaa gatgtaagta ttagatatgt agactatgga gaatctttag gaaatccaga
    24541 tatattaata atacctggaa ctaaaagtac tatagacgat ttaacatata taagaaaaag
    24601 tggtcttgaa tcgcaaatca aaaatttaca tagtcaaggt aaattgatat ttgggatatg
    24661 tggtggatac caaatgttag gtaaaaaatt aaaagaccct tatcatgttg aaagtgaaat
    24721 tgaagaagtt gatggaatag gtcttttaga tacagaaaca acgtttgaga aagaaaagac
    24781 tacaacacaa gtagaagcta ctattgttga atgtatcagt gaatacatga ataaccttaa
    24841 gggaaaaaaa gtaaaaggtt atgaaattca tatgggaatt acaaagcgag gtaatgatgt
    24901 aagaagttta gatttaataa ctaaaaaact tgataaagaa gtgaattata ctgagggaag
    24961 tgtaaataaa gaggggaatg taattgggac ttatcttcat gggatatttg atgaaataag
    25021 ctttacaaga gcaattttaa acaatataag aaagaaaaaa ggtttaggag aattagaaag
    25081 caaagtaaac tcttttaatg aatttaagga taaagagtat gataagttgg ctgatttttt
    25141 aagagaacat ttagatatag agaaaatata tgagattatg aaataatgat gacttttatt
    25201 tataatttag gtaggaggtg tattatgaaa aaaattttaa ttgcgggtac taatagtgga
    25261 gtaggtaaaa caacaatatc attaggaata atgcaagctt tggttaagag aaatatgaaa
    25321 gtacagccat ataaagtagg gccagactat atagaccctt catatcacac ttttataact
    25381 ggaagacatt ctagaaattt agattcttat atgttagatg atgaaaaaat aaagtatata
    25441 gtaaataagt cctcaaaaga tgcagatatt tcagtaatag aaggtgttat gggcttatat
    25501 gatggctttg gaatagattt agataattgt acaagttcac atacttcaaa ggtattaaag
    25561 tcacctgtta tattagttat aaatggtaaa tctatggcag catctagtgc tgctatggta
    25621 cttggatata aggaactaga taaagatgta aatataaagg gtgttatagt aaataatgta
    25681 aagactaata gccattatca aataattaag agttctatag aaaaatattg taatgttgaa
    25741 gtattggggt attttcctcc aaataacaaa ttctctttgg aatctcgaca tctaggatta
    25801 gttcctagtg tagaaatgaa atcactaagg gaaaaatttt ataccttagc agatgaaatt
    25861 gaacactata tagatattga cagaataatt gaaatatcag aaagcgatga atttgagagt
    25921 gattttgatt ttcaaacttt aatagaaaat aataaaataa aagaacatgt taataataaa
    25981 tcaatagcta tagcatatga taaagcattc aatttttatt atagagaaaa tatagagctt
    26041 ttagaggaac ttggtttaga aataaactat tttagccctc ttgaagatgt atcagtgcca
    26101 aatgcagatt atatttatat tggaggaggt tttccagaaa tatttggaaa agaattagaa
    26161 gcaaatgagt caatgagaac ttccatatta gaagcacata acaataatat acctatttat
    26221 gcagaatgtg gaggactgat gtacctaggt gagaaactat tgaatcaaga agaacaagaa
    26281 tttaatatgg ttggaatatt tagtggaatt agtaagatga catcttcttt aaagagattt
    26341 gggtactgta ttggtgtagc caaggaaaat actcttttat ctaaaaaagg ggaatctatt
    26401 aaagggcatg agtttcatca ttcaatattt gaaagtgatg aaaaatgtgt atattcaatg
    26461 agaaaagaaa gagatggaaa tatagtagaa gagtgggatg gtggttatag taaaggaaac
    26521 actttggcta catatcttca tactcatttt tataataact taaattgtat agaaaatttt
    26581 ttgaaaaaag gaaataagta aatgagtatt ttatctattt atataggata tgtgacagac
    26641 ttaattgtag gagacccata ttcttttcct catccagtga gatttattgg taaacttata
    26701 aattttactc aaggtataat aagaaaggtc tctaaaaatg ataggcagtt aaaatttggg
    26761 ggatttatat tatggtttat aaccgttgga ataacatatt taacaactta tgctatagtg
    26821 aagttattta gttttaatat attattgagt attgtagtga atagttttat aatttataca
    26881 acactagcca caaaatgttt aaaagacgaa gcattaaaaa tttataatgt attaaaaact
    26941 gaagatatag aaaaatctag gattcagcta tcttatatag taggtaggga tactactaat
    27001 ttaagtgaac ctgaaataat aagagcaact gtagaaactg ttgcagaaaa tactgtagat
    27061 ggtataattg cgcctatgtt ttatgcattt attggaggag caccacttgc tatggcctat
    27121 aaagctataa atacattgga ctcaacagtt ggatataaaa ataaaaagta taaagatatt
    27181 ggttttgcat ctgctaagat tgatgatata gccaattata taccagctag aatatctgtc
    27241 attcttatga caattggaag tttcttttta aattataact ataggaactg ttttaaaatc
    27301 tctattagag atagaaagaa tcataaaagt ccaaattgtg ctttttcaga aggtgcagta
    27361 tctggtgctc taggaataca actaggaggt accaacgttt attttgggga aaaagtatat
    27421 aagccaacaa taggggataa gcttagggag atagataaag aagatataat taaaactaat
    27481 aaaattatgt atgcatcttc atttgtatct atgttggtat ttacactaat atatgtgtta
    27541 tgtaggatga ttttaattaa cttaattata taaattattg tttgttaact taagggggac
    27601 atagatgaag gatttagggc atggtgccaa tgtagatgaa atggcaagat tatatgggaa
    27661 aaacccaaaa gaaataattg attttagttc aaacataaat ccaaatgtat taccaaattt
    27721 agagagatac attttaaaag gcttagaaga gtgtaggaat tatccagata taaattatac
    27781 aagcttgaga gaaaatatat caaaatatat agatataaat ccagacttta taatacctgg
    27841 caatggtgca acagaagtga tttatttgct tatgaagagt attaaaaaaa aattggctat
    27901 aataaatccg actttttcag agtatagaag aagtgctgag ttaaataatt tagatattat
    27961 agatttagag ttagatttag aaaataactt taaacttaat atagatataa taaaagaaaa
    28021 tataaaaaga tttgatagtt tatttatatg taatcctaat aatccgagtg gtaatatcca
    28081 agatttaaag gaacttgttc atttactaga taaacataat aaggtactaa taattgatga
    28141 aactttcatg gaatttgttg aagatgaaag taaatatagt ttggttaaat atatagaatc
    28201 aaataaaaat atatttataa ttaaagctgt gactaagttt tttggtatgc ctggtttaag
    28261 gctaggatat ggtcttacaa gcaacactga aattatgaac aaaatttatg aacacaaaga
    28321 gccatggact atcaattctt ttgcagatat actatccaat tttatttttg aagataaaga
    28381 gtatattaaa aatagtaaag aatattacat agaagaaaga aaatatgtgt tacaagagtt
    28441 gagaaacata agaaatataa aggtctatga tacagatgct aattttatct tgataagaat
    28501 atataaaaaa acaactaaag aattgaaaaa agatttgttt aagcaaggaa acatattagt
    28561 tagagatgcg tctaatttta taggattaga tgatagtttt ataagagtgg ctataaaatc
    28621 tcatgaagat aacaaaattc ttatagaaaa tatgaaaaat ttattgggtg attaaaatga
    28681 aatcttatgg aatatgccca gcatcttgtg gagaatttgt acaaggtata attgatgatg
    28741 aagaatattt atgttcatat gcaatagata tgtattctaa agtgtatatt gaagaaaaat
    28801 tagtagatat aaatttaggg agatataagt caaggttagc aattgaaaag gtatttgaaa
    28861 aatttaattt acctaaaaaa tatactaaaa atatttcttt aaatataaat agtaaaatac
    28921 ctgttggaaa aggaatggca agttcaactg ctgatatagg agctactata aaggcaacat
    28981 tatcattgat agataaagat ttaagtagcg aggagatttc taaattagca gcagaaatag
    29041 aaccaacaga ttctatattt atagataaaa atagtatatt taatcctcta aatggaactg
    29101 ttataaaata cctaggaaat ctgaccaatg ctaaagttgt aatacttgaa ccaaacaaag
    29161 tactagatac tatgaaaatt agattgaggc aagattataa taaattaaaa gtagagaata
    29221 aagaagtaat aaaaaaatca tttgctcttt tagaagaagg attgaaaaaa aataatttat
    29281 ctctagttgg agaggcttgt actttgagta gcttagcaaa tgagaatata gaaaaaaaag
    29341 agtatttaaa tgaaataata aaaatatcaa aaaaatatgg agcatatgga gtaaatattg
    29401 ctcacagtgg aactgtagta ggtattttaa ttgataagta tatgaatgat aaaaagatga
    29461 tagatgcctt atgtgaatct aacataaatt ctgtctataa taaaatttat acacaaaaca
    29521 taattaatgg aggaattaag ggggaaatag aatggaatac ataaagaatc ctatgaaaat
    29581 agaagaaaaa agttttgaat tgatacaaga aataatagac gaaattagac cagattataa
    29641 atttaagaat aatatcgaag aaaaaataat aaaaagagct atacatacta ctgctgattt
    29701 tgattactta gatatattaa aaatatcaga agaagcagtt gatagtataa tagatgcatt
    29761 aaaaaataat gcaagtattt atactgatac aaatatggct cttagtggta taaataaaag
    29821 aaagttagaa tcattaggat gtaagtataa atgtttagtg gcagatgatg aaactgtaaa
    29881 attagctaaa gaaaaaggaa ttactcgttc tatggcagca gtagaaattg cagctaaaga
    29941 agaaggaaga aaaatattcg ttttaggtaa tgctccaaca gctttatata aagttatgga
    30001 gatgaaatca gaaggtagat tagatttaga tgcagttata ggagttcctg taggttttgt
    30061 aggagcacaa gaatcaaaag atgaagttga aaaaacagat ataccatata taatttctaa
    30121 aggtagaaaa ggtggcagta atttagcagc agcaatagta aatgctatat tatatactat
    30181 gtaggtatct atatggaaga atatgtatat atagatggaa aaaaatatag aagaggatat
    30241 actacaggtt catgtgcaac aggtgcatct aaggctgctg tatatatgtt gattacaaaa
    30301 aatagaatca atactataaa tatagatact cctaagggga taccattact actaaaagtt
    30361 gataatataa atatttctga tacatttgtg gaatgttcca taaaaaaaga tggtggagat
    30421 gatatagatg caactcatac catggatatt tatgcaagag ctgaaattgt agctaaaaat
    30481 gataagaaca aagggtatct tactctaaag gatatagata gtttaagcac caatagtgag
    30541 tgtaaaagtg agctctataa gtttattagg gtttatggcg gaactggtat tggtgtggtt
    30601 acaaaaaaag gtcttagcgt ggatgttggt aaacctgcta taaatccgac tccacttaaa
    30661 atgataaatc atgaaataag aaagcttata ggagataatt ttgaatctat acttggaaat
    30721 gataaagttt taaaaataac aatctttgct ccacaaggag aaactgtagc aaaaaaaaca
    30781 tttaatccaa gattaggtat agttggagga atatctataa taggtacaac tgggattgtt
    30841 gaacccatga gtgatgatgg ttggaagaag tctctatcta tagaacttca gatgaaaaaa
    30901 gaacaaggct tagataaaat aatattggtt ccaggaaatc atggagaaca gtttataaga
    30961 gaaaaattaa acttagatat taagtatgta gttagggtaa gtaattttat aggctatatg
    31021 attaaagaag ctcaaagaat aggatataaa aaaatcttaa tggcaggtca tataggtaaa
    31081 tttataaaag tatctgctgg tatttttaat actcatagta aggtagcaga tgctagaagt
    31141 gagatattag tggcaaactt agctttaatg ggagctagat acgaattttt aaataaaata
    31201 aatcaatgcg taactactga agaagctgta gagctaataa ataatagtga atatagagaa
    31261 gtatataata tattaagtaa taaatgtagg gagagagtaa agcaatactt gaatgaagat
    31321 tcagatgata ttgatgtgga agtaatcata ttttcaatgg ataagtcttt attaggtaaa
    31381 tcggataata cagatgattt agtggaggtc tttatatgat aaacataata ggtctaggac
    31441 ctggtaactt agactatata acaaaaaaag gtgaaaattt aatttccact tctgatgtat
    31501 taattggtgg taaaagaaat ttagagtcta taaaaaattt tgaaggagaa aaaatagttc
    31561 tggattctaa tttaagagaa attatagagt acataaataa caataaagaa aagcaaatat
    31621 caataatagc atctggggac ccactaatat atgggatagg tagatatcta tctaaaaata
    31681 ttgataataa gatgttaaac atggtttctg ggattagttc attacagtac atattttcaa
    31741 aaatatatgt agatatgaat gatgtatata ttacaagtag tcatggtaaa gtaccagatt
    31801 ttgattacat attatcacat aaaaaagtat gtatggttac agattcaaag ataggaccaa
    31861 aacaaataag tagagaaata atagatagaa atctaaataa aattattgtt gtaggagaaa
    31921 atctgtcata tgacaatgaa aaaataacaa tagcaaaacc agaagagata ataagaatgg
    31981 acaattttga tatgaatgtt gtggtgattt tagatgagga atagtgaatt tataacaggt
    32041 aaagttccaa ttactaagga agaggttaga gcaatatcaa taagtaagtt aaatttagta
    32101 aatgctaaaa acctcataga cataggggct ggtacaggaa gtgtaagtgt tgaagctgca
    32161 tataattatc ctaatttaaa ggttatatct atagagaaaa atgatgatgc tatagagctt
    32221 atagagaaaa ataaaaaaaa gttcaactta aaaaatatag aagtgataaa aggatatgct
    32281 ccaattaatc tagggttaaa tgttaaagtt gagtctattt ttcttggggg aacaggaaat
    32341 aatctggaag aaataataga atggtctaaa aaaaatctcg ttacaggagg aagacttgta
    32401 gctaatttta taataataga tacatttaat caaactttaa aactgcttaa aaagcatgga
    32461 tttaaagaaa tagatgtatg tgttttgaat gtttctaaac tagagaaact aggaaaaggt
    32521 gaatatttta aaccactaaa tcctatatat ataatatctt gtgaaaaagg ggaaaatgat
    32581 gatgaataaa gtacattttg taggagcagg acctggagat aaagagctga taacattaaa
    32641 aggatacaaa ttattaagta atgcagatgt agtgatatat gcaggttcac ttgttaatcc
    32701 tgaacttttg gaatactgta aagaagattg ccaaatacat aatagtgcac atatggattt
    32761 acaagagata atagatgtta tgagagaagg tatagaaaat aataaatctg ttgtaagact
    32821 gcaaacaggt gatttttcta tatatggttc aattagagaa caagtggaag atttaaataa
    32881 attgaatata gattatgact gtactccagg agtaagttct tttttagggg cagcatcatc
    32941 tttaggggtt gaatatacag ttccagaaat ttctcaaagt gtaattataa caagaatgga
    33001 aggcagaact ccggttccag agaaagaatc aatacaatca tatgctaagc atcagacatc
    33061 tatggtaata tttttatctg ttcaagaaat tgagaaagtt gtgtctaaat tactggaagg
    33121 tggatatcca aaagatactc ctatagcagt tatatacaaa gcaacttggg cagatgaaaa
    33181 aatagttaaa ggaactttaa gtgatatagc tgtaaaagtt aaagaaaaca acataaataa
    33241 gactgcttta ataatggttg ggagattttt aggagaagag tataataatt caaagttata
    33301 tgataaggat ttcaaacatg aatatagagg gtaatattgc tataatctgt attacagaaa
    33361 atggaaaaaa attagctcta aagataaatt ctctagtaaa taactgtgtt atatatcaag
    33421 taaaaaataa aaaaagtaat ttagttgttg aagaaacatg tgtgcataca gttcaaaaga
    33481 agttaagtga ttttgtagga gaaatattta gcaaatttga ttacatagta ttcataatgg
    33541 ctacaggtat agttgtacga agtatagcac catacataat aagtaagttt agtgaccctg
    33601 caattttggt aatggatgaa aaaggcaaaa acgtaataag tttattgagt ggtcatattg
    33661 gaggggcaaa cagcttgaca cagtacataa gtagtattat tgatgctaat ccagtaataa
    33721 caacagctac tgatataaat caaaaagctt ccttagatat gatagcaaaa aaactaaatg
    33781 cacatattga taattttaga gaaagtgtaa aagaagtaaa ttctatgcta gtgaataata
    33841 aatgtgttgg attatatgtt gatgaaggat atcaagttga tactagggga tttaaactta
    33901 tagaaagtaa ggatgagtta agagatagtt tttcaaacgc atatatagaa aaattagttg
    33961 tagtaagcaa taaaataaat tgtattgaag atattgagaa tttaaaccta gataataaat
    34021 ttgaacattg ctatagtacc caaaaaataa taaaggtaat tccaagagat atagtgatag
    34081 gaattggttg tagaagaaac acagatagtg aactgatgta taattcactt gaagagtttc
    34141 ttaataaaca aaatacagat attagagcaa taaacacagt tggaagtata gaactgaaaa
    34201 aagacgaaaa agcaataatt gaattatcaa aaatattgaa ggtgccattt aaaacatttt
    34261 cagtagatga aatatctaag gttgataatc tttttgataa atctgatttt gttaaaaaaa
    34321 atgtgggcgt gtattcagta gcagaacctg tagcatatct tttgagcaaa ggtaatttgt
    34381 tagttgaaaa acataaatat aaaggaataa ctttttcagt ggggagaata aaaatatgat
    34441 atacgttgta ggaattggac caggaagtaa agatacaatg actttagaag caataaaagc
    34501 aatagaagat tctgaggcta ttgttggata taagacttat ataaaactaa tagaagaatt
    34561 tatacaaggt aaagaaataa tccaaaatgg tatgaggcaa gaagttgata gatgtaaaca
    34621 agcagtagag gaagcaaaaa aaggaaaaaa agtagcagtt gtaagcagtg gtgatgctgg
    34681 aatatatggg atggcaggtc ttattttaga attaatatct aaagaagatg aagatataaa
    34741 agtaaaggta attccaggtg ttactgcaag tattggtgca gcagctatac ttggagctcc
    34801 tattatgcat gatttctgtc atataagttt gagtgattta atgactccgt gggaagtaat
    34861 tgaaaaaaga ttgagattgg ccgctgaagc cgattttgta atttgtttgt ataatccaag
    34921 aagtaaggga agaagtgaac atctaagcaa agcatttaaa ataatgggtg agtttaaagg
    34981 tggagataca cctgttggag tggtaaaaga tgtaggtaga gaaaaagaag aaaagtttgt
    35041 atgtactttt gaaaatatgg actttgaaaa agtcgatatg actacaatgg ttataatagg
    35101 aaataagtct acctatatta atggtgaatt gatgataact ccaagaggtt atacagtatg
    35161 attttaattt taggaggaac ttcagatagt ttatctatat gtgataagat aaatgaactg
    35221 aaaaatcaac catatatcct ttcagttact acagattatg gaagggaact tgcacaacaa
    35281 catgcagaga atgtaatttt aggtaagctt ggaaaagaag atatgctaaa atttataaaa
    35341 gataataata tagtaaaaat aatagatgcc actcatcctt atgcagttga agtatccaaa
    35401 actgctatta cttgtgcaaa actattgaat atagattata taaggtttga gagaaaatct
    35461 ttaattgaag aaatagatta tgataataaa tttatagtag atacaattga agaagcctgt
    35521 gaaatagcaa ataaaatagg gacaaatata tttataggaa caggaagtaa aaatttgaac
    35581 agatttatag aggaaataag tgataaaaat ttagttgtta gagttcttcc aacgagtgaa
    35641 gtcatattaa gttgtgaaaa attaggttta agtgcagata atatagtagc tatgaaaggt
    35701 ccatttagcc agtatataaa tgaagaaaca tataaacatt acaatataga tttagtcata
    35761 acaaaagaaa gtggaatagc tggtgggttc ttagagaagg taaatgcatg taaaactttc
    35821 aatataccag taataattat aaaaagagaa tcgattaact acgaaaatgt atttaatgac
    35881 ataaataaaa ttaaagacat acttgtgtaa tttttaggag gagaatatat gaagaaagcc
    35941 ttattagtag tgagttttgg tacaagctat catgaaacga gagaaaagac aatagatata
    36001 tgtgaagata aaataaaaaa ttcgttaaaa agccatgatt tttttagagc ttatacatca
    36061 aatatgataa taaataaaat aaaaaaaagg gatggaatag aaatagataa tccaatacag
    36121 gctttagata aaatatatga acaaggatat gatgaagtaa taatccaaac acttcatatt
    36181 atatgtggcg aagaatttaa caaattaaaa gaacaagtag atggatattc ttctaaattc
    36241 aaaaagattg tattaggaag acctttgctt acacatattg aagattatca ggaagctgta
    36301 gaggcaatta agcatcaaat tcctcatatg gaaagtaatg aagcagtagt atttatggga
    36361 catggaacac ttcatgaatc acattcagcg tatccagcat tggaatacat gcttagggat
    36421 aatggaataa atgcttatgt tggtacagtt gaagggtatc cagaaataga acatgtaata
    36481 agaagattaa aagaaggaaa tataaaaact gtaaatttga tgccatttat gttagttgca
    36541 ggagaccatg ctataaatga tatggcagga gatgaagaag attcttggaa aacaatatta
    36601 gaagaaaatg gatttgacgt aagagttcat ttaaaaggac taggagaaaa ttcttatata
    36661 caagataaat ttgttcgtca tgctgtaaaa tgtgcagaga atgcgaagtt ctatggtata
    36721 ggagcagggc caggagatgg ttctttactt acgataaaag cggttaatac tttaaagaaa
    36781 ctggatattc tatacactcc agaatcaaaa aagggtggag atagtctagc tttatcaata
    36841 gttagtgaat atttacctga atcagtggaa ataaagtcta ggcatttccc aatgagtttt
    36901 aatgggaatg aaaaaagttt agcttgggat aatgtagcac aagaaattat atcagatgtt
    36961 aatgatggta aaaatgtagg atttgttaca ttaggagacc ctatgattta tagtacatat
    37021 gtatatataa tgaaaagaat attaggagag gtagaagtcg aaactatacc aggaatttca
    37081 tcattttcca atatagcatc taatcagaat tttccattag tcatggacaa agaagcttta
    37141 gtggtaattc cttgtactat ggaagatgat aagatagagt atgctttgca aaattataat
    37201 tcaatagtat tgatgaaagt atataaaaat tttaaagaaa taataaaaaa attagaaaag
    37261 tatgatttaa tagaacatgc tattttagta agtaattctt cccaagaaag cgaaaatgtg
    37321 tttacagatt taaaagaagc gcaccttgaa gataagatat cttatttttc tactatacta
    37381 gtaaataaag ataataaaat aaaataggtg aaagcaatat ttaaaggaga cttaatatga
    37441 atatagtggt tggaactagg ggtagtaatc tagcgcttat acaaacagaa tgggtaataa
    37501 atgagttaaa aaagaagtat ccagaaatta gttttgagat taaaatcata aagacaaaag
    37561 gagatttaat tcaaaatgtt tcactggata aaataggtga taaaggattg tttgtcaaag
    37621 aaatagagca acaattatta gatggaaaaa tagacatagc agtacatagt atgaaagata
    37681 tgccttctta tttagcagat ggactaaaat ttgctcatac gcctaagaga gaagacccaa
    37741 gagatgttct aatacttaga gaaggatata aaaatttaga tgatttgcct catggagcag
    37801 taataggaac aggaagtaaa agaaggaagt ttcaattatt gaaacaaaga cctgatttaa
    37861 atatagtaca agttagaggt aatgtggaaa ctaggatacg aaaaataaaa gatgaaaaca
    37921 tgcatggtat tgtgttggca gcatcaggaa taattagagc taatttacaa gataaaataa
    37981 gttcttattt acctgttgat gttgttattc cagcacctgc ccaaggagct ttagctatag
    38041 aaattagaag caatgatagt acgatagagg gaatagtaaa ttcactgaag gatgagaata
    38101 cagaaatcca aatattggct gaaagaggtt ttttagatgg tgtaaatgga agttgtcata
    38161 ttccaatggc agcttattgt gaaataatcc aagataaaat acatcttact ggcttatatg
    38221 gagattcaga aggaaaaaaa gtagtaatta agtctataga tggagatatt tcttctccta
    38281 gagagttagg tttaaagtta gctaaacttg tattgaagga gtatgagaat tatgaagggt
    38341 aaggtttact tagtaggggc aggaccaggt gattataaac tcatgacttt aaaaggttta
    38401 gagtgtataa gaaaatcgga tgtaatagta tatgatagat tagcaaatag tagttattta
    38461 agagaagcta agcctgattg tgaacttata tatgtaggca aggcatcaag taatcatatt
    38521 ttgccacaag aagatataaa tagaattata gcagaaaaag ctaaagaagg aaaaattgta
    38581 actcgtctta aaggaggaga cccttatgta tttggcagag ggggagaaga agcagagact
    38641 ttagtagatg aaggtataga atttgaagta gttccaggta taacttctgc tataggaggt
    38701 ttatgttatg cgggtattcc tataacacat agagaccatg cttcgtcatt tcatgtaata
    38761 acaggtcatt tgaagggtga tgatagtgga gaattaaact ggaatgctct tgcaaataac
    38821 aaaggtactc ttgtgttttt gatgggaatt tcaaacctag agaaaataag tgaaaactta
    38881 atgaaagaag gaaaagataa ggatacccct gttgctctta taagttgggc aactagatat
    38941 aatcaaagag ttgttacatc tacacttgaa aatgtttatg aaactgctat taaagaagaa
    39001 gtaaaaccgc caactcttat agtagttggg agtgttgtag gacttagaga aaagttgaac
    39061 ttctttgaaa gtaagccttt atttggaaaa agtattgctg taactaggtc tagaaatcaa
    39121 aatagtgtat tggtagaaaa aataatggat ttaggtggaa atcctataga aatacctact
    39181 attaaggtag aaaaaataca aaataatatt aacttagaaa atgaaataaa aaatataaat
    39241 aagtataatt atctaatact aactagtaaa aatgccgtag aaatcttctt tgaaaagata
    39301 tatgaaatga attttgattt aaggatatta tctaatttaa aggtatgtgc tataggttct
    39361 gctacatcaa atgagttgaa aaaaagagga attatagcgg atatagtacc aaaaaaattt
    39421 gtagcagagt cactgtatga agagttagtg cctattttaa atgatgacga taatatactt
    39481 atacctagag cagaaaatgc tagagattat ctggttgaca aacttaaggc tatttgtgaa
    39541 gtaacagaag tacatatata tagaacagta atagatgagg agaaaaaagg tgagatatta
    39601 gatattttaa attctaatga catagattat ataacattta ctagttcctc aacagtaaat
    39661 aatcttgttg aaattatagg aaaagaaaat attaaaatat tagaaaaaat aaaaacacta
    39721 tctataggtc ctataactac agagacaata aatagtttgg gaataaaatt gtacaaagaa
    39781 tctgctacat ctactataga ttcgttagta gatttaatta taaaaaatta attttggagg
    39841 cttgaatatg ttaagaagac caagaagact aagagcaagt aaagcaatca gaaatttggt
    39901 aagagaaact aaggtgaatg tagaagactt aatatatcca ctgtttgtag tagaggggga
    39961 aaatataaaa gaagaaatta aatctttacc agatgtctat catttatcaa tagacatgct
    40021 agaagacgaa ataaaagaaa taagtgagtt gggaattgaa catataatgt tatttggtgt
    40081 tccagatgaa aaagatgagt gtgcttcaca agcttttaat gataatggaa tagttcaaaa
    40141 ggcaattaga aaggtaaaag aaataaattc agatataaat gtaataacag atgtctgtat
    40201 gtgccaatat acaagtcatg gacattgtgg gattttaact gatactggtt atgttgataa
    40261 tgataaatca ttggagtatt tggctaaaat atctgtaagc catgctaaag caggagctga
    40321 tatggttgct ccatctgata tgatggatgg aagaatacaa gcaataagag aagctttaga
    40381 tgaaaatgga ttagaaaatg ttggaatcat ggcttatagt gttaagtatg cttcaaatta
    40441 ttatggacct tttagagagg cagctcattc agcaccatct tttggagata gaaaaacata
    40501 tcaaatggac ccagcaaata gcaatgaagc tataatagag actgaacttg atatattgga
    40561 aggtgcagat atacttatgg ttaaacctgc attatcatat ttagatatta taagaagagt
    40621 taaagataat tttgacttac ctctagcagc ttataatgta agtggagaat actctatgct
    40681 taagtttgct gtgaaggaag gtttattaaa tgaagatgta attcttgaat caattacatc
    40741 tataaaaaga gcaggagcag atataataat aacttatttt gctaaagaca ttgctaaact
    40801 gttgaaaaaa taaattctag tagtataaag aatatcaaaa agtataggaa ttgtaattaa
    40861 ctattataca gtagttttgt ttagacattt attgaagaat aatagaaact aatttgcaat
    40921 ttctatactt tatttatgta actaaataaa atttggaggt ggaagatgct atatcctatt
    40981 aatttaaaat tagatgagtt agatgtagtt ataattggtg gtggagaagt tgcatatagg
    41041 aaatgtaaaa attttttaga gtttaacaaa aatgttacta tagtttctaa acaaatttta
    41101 aataaatttt atgatttaaa aggaaatata aaaataataa aagatgatta taaagaaata
    41161 tatataaaag atgcttctgt aataattgct gccacaaata atagagagtt aaatatggaa
    41221 ataggattat attgtagagc aaaaaataag ctagtgaatg tagttgataa tatagaaata
    41281 tcgaacttta cggttccatc atatataaaa agaggagatt tacttataag tgtgtctact
    41341 gggggcaaga gtccttcatt atcatcaaag ataaaaaaag aaattgaaga gagatatact
    41401 gaagattatg aggagtattt aaatgtatta ggagatataa gaaaaaaggt tataaaaaaa
    41461 tacgaagata aaagtaaaag aaaagacgtg cttaatgtgt tggtaacact tgacctagaa
    41521 gaacttaaga agtttgatat ataaaaaata aaataaaaaa tgttaaaaag gtgttgacgg
    41581 attattttta ag
//
LOCUS       DAEORZ010000018        41532 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEORZ010000018 DAEORZ010000000
VERSION     DAEORZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.018"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            174..1604
                     /locus_tag="KRH39_000860"
     CDS             174..1604
                     /locus_tag="KRH39_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG8510783.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            1972..2550
                     /locus_tag="KRH39_000861"
     CDS             1972..2550
                     /locus_tag="KRH39_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG8510784.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            2580..2726
                     /locus_tag="KRH39_000862"
     CDS             2580..2726
                     /locus_tag="KRH39_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG8510785.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            3031..4410
                     /gene="scfB"
                     /locus_tag="KRH39_000863"
     CDS             3031..4410
                     /gene="scfB"
                     /locus_tag="KRH39_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBG8510786.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            4439..4936
                     /locus_tag="KRH39_000864"
     CDS             4439..4936
                     /locus_tag="KRH39_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBG8510787.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            5234..6898
                     /locus_tag="KRH39_000865"
     CDS             5234..6898
                     /locus_tag="KRH39_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBG8510788.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            6911..9361
                     /gene="recJ"
                     /locus_tag="KRH39_000866"
     CDS             6911..9361
                     /gene="recJ"
                     /locus_tag="KRH39_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG8510789.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            9374..9886
                     /locus_tag="KRH39_000867"
     CDS             9374..9886
                     /locus_tag="KRH39_000867"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBG8510790.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            9960..12167
                     /locus_tag="KRH39_000868"
     CDS             9960..12167
                     /locus_tag="KRH39_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBG8510791.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            12192..12641
                     /locus_tag="KRH39_000869"
     CDS             12192..12641
                     /locus_tag="KRH39_000869"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBG8510792.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            12656..13270
                     /locus_tag="KRH39_000870"
     CDS             12656..13270
                     /locus_tag="KRH39_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8510793.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            13361..14917
                     /locus_tag="KRH39_000871"
     CDS             13361..14917
                     /locus_tag="KRH39_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBG8510794.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            14907..16169
                     /locus_tag="KRH39_000872"
     CDS             14907..16169
                     /locus_tag="KRH39_000872"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBG8510795.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEKANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            16243..18030
                     /gene="aspS"
                     /locus_tag="KRH39_000873"
     CDS             16243..18030
                     /gene="aspS"
                     /locus_tag="KRH39_000873"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBG8510796.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            18489..19721
                     /locus_tag="KRH39_000874"
     CDS             18489..19721
                     /locus_tag="KRH39_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG8510797.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            19735..20541
                     /locus_tag="KRH39_000875"
     CDS             19735..20541
                     /locus_tag="KRH39_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBG8510798.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(20653..21522)
                     /locus_tag="KRH39_000876"
     CDS             complement(20653..21522)
                     /locus_tag="KRH39_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8510799.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            21894..23321
                     /locus_tag="KRH39_000877"
                     /pseudo
     CDS             21894..23321
                     /locus_tag="KRH39_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
     gene            complement(23398..23667)
                     /locus_tag="KRH39_000878"
     CDS             complement(23398..23667)
                     /locus_tag="KRH39_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510800.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMRGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(23670..25112)
                     /gene="nhaC"
                     /locus_tag="KRH39_000879"
     CDS             complement(23670..25112)
                     /gene="nhaC"
                     /locus_tag="KRH39_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG8510801.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(25137..26303)
                     /locus_tag="KRH39_000880"
     CDS             complement(25137..26303)
                     /locus_tag="KRH39_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG8510802.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKYYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(26528..28648)
                     /locus_tag="KRH39_000881"
     CDS             complement(26528..28648)
                     /locus_tag="KRH39_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8510803.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSNNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            28881..29282
                     /locus_tag="KRH39_000882"
     CDS             28881..29282
                     /locus_tag="KRH39_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510804.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            29325..29618
                     /locus_tag="KRH39_000883"
     CDS             29325..29618
                     /locus_tag="KRH39_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBG8510805.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            29633..30073
                     /locus_tag="KRH39_000884"
     CDS             29633..30073
                     /locus_tag="KRH39_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBG8510806.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(30165..30638)
                     /locus_tag="KRH39_000885"
     CDS             complement(30165..30638)
                     /locus_tag="KRH39_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG8510807.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(30638..31426)
                     /locus_tag="KRH39_000886"
     CDS             complement(30638..31426)
                     /locus_tag="KRH39_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG8510808.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            31754..32998
                     /locus_tag="KRH39_000887"
     CDS             31754..32998
                     /locus_tag="KRH39_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG8510809.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            33321..34418
                     /locus_tag="KRH39_000888"
     CDS             33321..34418
                     /locus_tag="KRH39_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG8510810.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            34409..35107
                     /locus_tag="KRH39_000889"
     CDS             34409..35107
                     /locus_tag="KRH39_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG8510811.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENNIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            35110..36174
                     /locus_tag="KRH39_000890"
     CDS             35110..36174
                     /locus_tag="KRH39_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG8510812.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            36357..37061
                     /locus_tag="KRH39_000891"
     CDS             36357..37061
                     /locus_tag="KRH39_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8510813.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            37054..38376
                     /locus_tag="KRH39_000892"
     CDS             37054..38376
                     /locus_tag="KRH39_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8510814.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQIYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            38563..39936
                     /locus_tag="KRH39_000893"
     CDS             38563..39936
                     /locus_tag="KRH39_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510815.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGSITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(40321..41259)
                     /gene="pdaA"
                     /locus_tag="KRH39_000894"
     CDS             complement(40321..41259)
                     /gene="pdaA"
                     /locus_tag="KRH39_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG8510816.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT        16000 a         4720 c         7071 g        13741 t
ORIGIN      
        1 aatgtccact attaacttta ttaccccaca tatataagat aaaattatta atataaaata
       61 taatctatcg acaatgaata gacttttaaa taaacttaat ataaaattaa ttaaataagt
      121 gcttaaaaac gatttacttt aatgatagaa tcatattaaa ggaggataat tttatggata
      181 taaagaataa aattaaatct atatttttac cagaaaaaaa tgactattta gatgatgaag
      241 aatttgagta tgatatagaa aataatgaag aagatttaaa ggaagataac aaagtacatt
      301 taaatgattt aagcagagta aaatatgatt acagagagtt aaaagacata gaaaaccaaa
      361 cagaagagat gtgtctgcta gcagtaaagc aggatggtac tataattgag tttgtaaagg
      421 acaagactta taaagtatgt atggaagcag taaaacagac atataaatct cttaagtata
      481 taacaaatca aaatgaggat atttgtatag aagcagtaaa acagaactac agagctttgt
      541 actacataaa taataagaca gaaaatgtac taattgaggc tataaaaaat gcatcaactt
      601 atgatgtcat ggaagtattt aaatttgtgg aagagcaaac agaagatgta tgtctagcat
      661 ttatagaaag ggcatctaaa aatgatgttg cagagatatt aaaaggaata aaagagcaaa
      721 ctccagccat atgtttagaa gctgtaaaaa aagatggaaa atctttagct tatgtaaagg
      781 aacaaagtaa tagtatatgt ttagaagctg taaaagaaaa ctacagtgca ctatcttgtg
      841 taaaagagca aactgaagaa atatgtatag aagcagtaaa acagaatgac tttgcattgt
      901 attatgtaaa tgaacagaca gaaaaaatat gtatggaagc agtaaaaaga agttatatgg
      961 ctctacagta tgtaaataag caaacagaag agatttgttt ggaagcagta agaattgatg
     1021 gaagagcatt acagtacgta aaagagcaaa cagaagaaat ttgcttggag tcagttagac
     1081 aaaatggaaa agtactgcaa tatgtaaaaa aacagactga aaatatatgt atagaagcag
     1141 taagaggttc atttgaagaa cttgaaataa aagaaatatt aagttatgta aagataccaa
     1201 cagaaagaat atttgtagaa gcagtaaagc aaaatggaaa aattcttaaa tatgtagaaa
     1261 atcagacaga attaatatgt ttagaggcag taagagaaaa ctataatgct ttagcttatg
     1321 taaaggaaca aacagaaaag atttgtttag aagcagttaa tcaaagttat gaagcgctta
     1381 aatatgtgaa agaacaaaca gaagaagttt gtctaaaagc agtaaaacaa gattatagaa
     1441 tgttgaaata tgtaaataat caaacagaaa aaatatgttt agaagcagta aagcaaaatt
     1501 atagagcttt agaatttgtt gataatcaaa cagaaaaagt ttgtcttgaa gcagtaaaac
     1561 agaatagaaa agcactacag tatgtaaaac aaaaacaaag ttaaaaggct aaaatatatg
     1621 gggaatatat gcaaataaat aaaatttgat attttgtaat aaataaaatg ggtatctcaa
     1681 aaggaacttt ttagtttgtg aggtactctt tttatatgaa atgaaatata tttttagctt
     1741 tttaacaatg gggaaaaggt ttgtttctat tttgacatag gctttttatg catataaagc
     1801 ttgataaaat tgttttatta taattgtttt gttatataaa taataagaag aaaattatat
     1861 aggtcttttt ttgaccagtt ttacattttt aacaactagt tttgtaaagt atattgaatt
     1921 ttctcaaaaa agaatgtact ctatatatgt aaaatttttt tggaggtaaa aatgtttaaa
     1981 agatatgctg aaaaaatgac atctgtttta atttgtaata atatgataga taataatgaa
     2041 tctaaagtct attcttatgg atttgaaata ttaatagctt tcatagttaa tataaccacc
     2101 atgctgttta taggtttttt atttggtaaa tttacatatg tattattttt tttaatgtgt
     2161 tattgtccaa taagacaatt ttcaggaggg tatcatgcag acaattattt tagatgctta
     2221 ttaaccttta tttttataat tttatctaca atcttaatta tagaaaatat aaatatagac
     2281 ttatttaaaa atattattat gattattgca agtgttagtt gggttggaat ttgtgttctg
     2341 tgcccaatag aacataggag taaccctata agtgataggg aaaaattggt ttataagaaa
     2401 actgctatat ttatatctac agtagtgtta ttgataacaa ttttaagtct ttctttgagt
     2461 atatttgtag attatttcac atatagcgca tttgccatgt tttggatttt tgtgatgtta
     2521 gtattaggta agttaaaagc taaagtataa gtaaaatatg tatgagggag agttaagtta
     2581 tgaaaaagtt tattgtaaga tttatgaagt ttgctagctc attggcactt agcactgcta
     2641 ttttgtcagc taatagtacc tgtccttgga ttattcatca gcctaaagta cccaaagaaa
     2701 tcagcaatct aaagaagaca aattaaatac aagcacttat ttatacaaaa ttatcaggaa
     2761 aaaagaaatt attatatttt aataagatta aaacaatata aaatcagata caaaatttat
     2821 tttatttatt ttataggagc ctaataccta tttggtaata tattagtgcg ccaatttagg
     2881 gggatatagt gtatttaatg aaatgactta cggagagtaa gtcatttttg tattgtatgt
     2941 attttatgta aacttatgat ataatgaagg gtaaagttta attaaaataa taaaaaactt
     3001 tagtatagat agtaatttag gaggtagggt atgagtatga tacataaatt ttctatgaat
     3061 ggatataata tcgttttaga tgtgaatggt ggagctgttc atgtgttaga tgatgttgca
     3121 tacgatttgt tagattttta taaagaaaaa agtaaagaag aaatattaga gatattaaaa
     3181 tcaaaatatc aagaagaaaa aataaatgaa gcatatgaag aaatattaaa tttggaaaaa
     3241 gagggtcttt tatatacaga agatacatat caatatcatc caagctttgt acatagagag
     3301 cctgtagtaa aagctttatg tttaaatgta gctcatgatt gtaacttaaa atgtaaatac
     3361 tgttttgcag ctcaaggtga ttttggagga gaaaaagaac ttatgagttt tgaagttggt
     3421 aaggcagcaa ttgactattt gattgctaat tcaggaagta gaaagaacct agagatagat
     3481 ttctttggtg gagagccttt gatgaacttt gaagttgtaa aacaactggt tgactatgga
     3541 agaagtgtag aaaaagatta taataaaaat ataagattta caataactac aaatggtgta
     3601 ctgctaaatg atgagattat agattatata aatgaaaata tgcataatgt agttttaagt
     3661 ttagatggaa gaaaagaagt taatgataat atgagaccaa ctttaaatga taaaggaagt
     3721 tatgatatta cattaccacg atttaagaaa cttgtggaaa aaagagcaaa agataagtat
     3781 tattatataa gaggtacttt tacaagagat aatttagatt tttcaaaaga tgttatgcat
     3841 tttgcagatt taggatttaa attaacctct gtagagccag tcgttggaga tgagagtaat
     3901 ccatatgcac ttagagaaga agatttacct aagatatttg aagagtacga aaaatttgct
     3961 gtagagtatg cagatagaca attacaagga gatggattta aatttttcca ttttatgata
     4021 gacctaaatc aaggtccatg tgtaataaag agaataactg gatgtggagc tggaaatgag
     4081 tatctttctg taactccaaa tggagatatc tatccatgtc atcaatttgt tggaaatgaa
     4141 gaatttaaaa tggcaaatat atttgatgaa gaaatagtac ttccagaaaa tttaaaaaat
     4201 atgtttagag aagctcatgt atatactaaa gaagagtgta aacaatgttg gaataagttt
     4261 tactgttcag gtggatgtca tgcaaatgcg ataaacttta ataatgacat atcaaaacca
     4321 tatgaattag gatgtgaaat gcaaagaaaa cgtacagagt gctctataat gatacaagct
     4381 aagttaatgt tagaaggagc aaccaactaa atttatattt taaaggagtt gtttatatat
     4441 gattagagat tatttagaag ataaaccttt aattgacgaa agtgtatttg tggcaaaatc
     4501 agcagatgta attggaaatg ttaaaatagg caaagattca agtatatggt acaatgcagt
     4561 agttaggggt gatgaaggac caataactat tggagaaaac actaatatac aagattgttc
     4621 tatagtgcat ggagatacag aaactataat tggtaacaat gtaacagttg ggcataggtc
     4681 tatagttcat ggttgtaaaa ttagtgataa tgtgttaatt ggtatgggtt caataatatt
     4741 ggataatgct gaaataggag aatatactct gataggagca ggtacactaa ttacatctaa
     4801 taagaagttt ccaccaggtg ttttgataat gggttctcct ggtaaggttg ttagagagct
     4861 tacagaagaa gataaaaaat atattgatga atcttatgaa tggtatttag aggcagcaca
     4921 aaatcaaaaa tattagttta aagagtggtt gtttcaaaac atatttaaat ctgttttgag
     4981 gcaaccacta atttatttta ttagattttg ttgatatttt agttttcaaa aagtatttga
     5041 ttaaaccaga tataaaatgt caaaaataaa aattaactga gttaaataat tttaataaaa
     5101 ttggtagtat atgtgataaa attaaatatt aaagaggata taggatatta gaatatcaat
     5161 tggtgaaata atgtcatata cgataaatta aggtgtaagt ttttgggtat acctatctta
     5221 ggagtgaaaa gatttgagaa taagttatag aaatttaaag cgatatagag aaattggata
     5281 tgttttaata aagtatggtt ttagttttat tgtagaaaga ctaaatatag aaggaattgc
     5341 ctataaaata ccactatttg acccaccaga agaaataaaa aatatgacaa ctggtgagag
     5401 aatgaaaaga gttttagaag aactaggacc tacatatata aaaataggac agatattgag
     5461 cacaagaaaa gacttgttag accaggatat aattgatgaa atatcaaaac tcagagatga
     5521 tgtagagaaa tttgattcaa atatagctat tgatatattt aaagaagaag tgggtttaag
     5581 tatagaagaa atattcttag aatttaaaga agaaccaata gcagcagctt caattggtca
     5641 agtttatgaa ggtgtactta aaacgggaga agaagtaatc gtaaaaattc aaagaccaaa
     5701 tatagagaaa ataataaaat ctgatttaga gattcttaga actattgcaa atactttaaa
     5761 agatttaaaa aaggatttta atttagattt agtacagatg attgaagaat ttcaaactca
     5821 attaatgagg gaacttgatt atacatttga agctataaat gcaactaaat tctctaggat
     5881 atttaaaaat agtgatgaag tatatatacc taaagtatat tcagaatata atactaagaa
     5941 aattttagta atggagaaag ttaatggtac aaagttaagt gatgtagaaa aaataaggag
     6001 actaggttat aacacaaaaa ctattgttga aataggcgtt aggtcatttt ttacacaggt
     6061 attatctcat ggattttttc atgcagaccc acatccaggt aatatattcg tagttgctaa
     6121 aaataagata gcatatatag attttggaat gattggaatt attgataata aaactttaaa
     6181 ccaattaaat gaaattgcat tagcaggtgt agaaaagaat gttgacaaga taatatatct
     6241 attgatagaa atggatgcat taaatggaga ggctgatatt aagggactta gacaggattt
     6301 actttatcta atacattatt actatgatat ttctatagaa aaaataaatg taacagatat
     6361 attaaatgaa ctatttaggt tttttagaca gtataaaata gtcatgcctg cacaatttgt
     6421 aactcttgca aagacagtta taacattaga aggaacaagt agaactttaa acacagattt
     6481 ttcttttggt tctatgggaa aagagtttat gaaacatcat tataagagta aatttaaccc
     6541 taaaaatgtt gttttaagtt caagacaaaa tgtagaagaa atattactgg acataaaaac
     6601 tattccaaag caattaaaag caattttaaa aaatatagaa agaaataata taaaaatgca
     6661 aattgaagat gttaaaatga cgaggttaga aaattgtata atagagctaa catctcaaat
     6721 atctttgagt ttagtgttgg catctattat agtaggttct tctcttataa ttgcatctcc
     6781 aaatatagaa aataatatat ggataaaatt tactgcaata gcgggtttct ttatatcctt
     6841 tataataggc ctttgcttag ttataagaag tattaggtca aaatacaaaa aagattaggg
     6901 ggaattaaat ttgaaaaaat ggatattgaa gtataaaggt aagatagagg aaagtgaatt
     6961 tagcaaaaaa cttaatattt ccccggaaat ctgccaaatt ttaaaaaata gagggatatg
     7021 tactgaaaaa gattcagaga tatttatgaa tccatcttta gattatttaa gagacccttt
     7081 tttaatgaaa gatatgcaaa aagctgttga tagaataaag ttagcagttg agaagaacga
     7141 aaaaatttgg atttacggag attatgatgt tgatggtgta tcatctacat ctatcttgtg
     7201 cttatatttt aagagtatag gttacaatgt tgattactat attcctaata gattagaaga
     7261 agggtatggt ataaatgaag atgctattaa gcttataagc tctaggggct gtgacttgat
     7321 tataagtgtt gactgtggaa ttacatcagt gtctgaggtc aatacagcga atgatttggg
     7381 catagatgtt ataattacag accatcatga atgtcaaagt gaaattccat cagcctttgc
     7441 agtaataaat ccaaaacaag aagattgtaa ttatccattt gattcactat gtggatgtgg
     7501 tgtagcattt aaaatgattc aagccttgac tccaaaagaa gaatttaaaa ctagtatgta
     7561 taattattta gaaatagtaa cactagcaac tatttgtgat attgttccac ttatagatga
     7621 aaatagaata attgtaaaaa atggtttaaa atcaatgaaa gaaggcaaaa atattggatt
     7681 aagggagctt ataaaagttt gtggtgtaga aagtgataag ataggttctt ctcatatagg
     7741 ttttgcaata ggtcctagga taaatgcttc aggtcgattg ggatattctt acttaggagt
     7801 agagctattt actactcaat cacaagaaga ggcagtagaa atagctagta tattggaaga
     7861 aaaaaataat gaaagacaga tgatagaagc taagatgtat catgaagctg aagaaatgct
     7921 aaagtctaat agcagataca atgatgacaa ggtgttggtt ttagctaaag agggttggca
     7981 acatggaata ataggaattg tagcatcaaa gttaactgaa aaatactaca aaccaacaat
     8041 tcttctaggt atagaaaatg gggaggctac tggttcagcg aggtcaataa aaggatttaa
     8101 tatatttgaa gctctcataa agtgcaaaga cttgatgact aagtttggtg gtcatgaaca
     8161 agcagcaggg ttatccttgg attcagacaa tgtagaaatt ttagcaaatg aaataaataa
     8221 atttgcagat tataatttaa ctgaagatga tatgatagag aatgtaaatg ttgagtttga
     8281 attacaagaa aatgtaataa atttaaatct tgtagaagaa ttacataagt tagaaccctt
     8341 tggattaaat aatcctaatc ctagatttat agttagaaat tatattttaa aagatttgaa
     8401 agttataggt aagaatcaac aacatctgaa attaagtata gaaaaagaga aaagctatga
     8461 atgtatagga tttaatatga gtcatttaaa atcaatgtat aaagtaggag acaaggtaga
     8521 tgtattattc caattagatg aaaataacta tatgggaaat aggaaagtac aatttttact
     8581 taaagatata cgattggctc gacctaaaag tgcaagtaat gacaaacttt cattgaaatt
     8641 aatgtcaaaa ataataccaa aagatactca aagcctgtat aatatatctg tgtctgattt
     8701 tgaattattt gatggaaata cagatataaa tatatttgat tattttgaaa aagatacact
     8761 aataatatct aattctataa atggatttta tagagcaatg tcagatatat cacttataga
     8821 tttggatttt aacataaatt acaatattat tgaagatgtt agcaaaaata ctgataaatt
     8881 agagcttata ttttctccta atattgataa aatagactta aaaagatata ataatattat
     8941 tctttatgat tatttataca ataaaggcga atattcgtat atatatgaga ataaaagaga
     9001 agaaagtgaa attataaaat attataataa aacagaccta ttatatttaa agaatgtagt
     9061 tagtaatata gttccatcaa gagatgaatt tataacaatt tataaacaag cactcataaa
     9121 aaaagaaatt gatttggata tggtaaatat aagggaaact tttaatgtaa ttccattaaa
     9181 gttttttacg atattaaatg tatttagaga attaaattta cttgacttta atttaaatta
     9241 tgaaaaaaat tctgtactca taagaatatt gccaaaacca cagaaaaaat tagacttaaa
     9301 tgagagttta atactcaata atctaaaaaa tttagaaaaa caatacaata gcagttatta
     9361 ggaggcgttt cccatggatt taaaaaactt tataaggaat attgatgatt tcccaaagcc
     9421 aggcatagat tttaaagatg tgacaactct atttaaagat ggagatgcat ttaaatatgc
     9481 agtggattct atagttgaag aattgaaaga taaagatgta gatttagtaa taggaccaga
     9541 agcaagagga tttttgatgg gtacacctgt agcatatgct ttaggagtag gatttgttcc
     9601 aattagaaaa cctggaaagt taccaggtga agttgagagt tatgagtatg gattagagta
     9661 tggtacagat actttagaga tacataagga tgcaattaaa aagggtcaaa aagtagcaat
     9721 agtagatgat ttattggcta ctggtggaac tatggaagcc gctgccaagc ttgtagaaaa
     9781 attaggtgga gaagtagttt ctatgcaatt tttgattgag ctaaaattct taaatgggag
     9841 agaaaagttg tctaactatg atgttaattc tcttataaaa tactagtact tttataatag
     9901 ttggtataac accaactatt ttcatatata ttaaaatttt atacaaaata ggtggttata
     9961 tgcatgataa agaattacaa gaaataattg aaaaaataaa agtttatgca cctaatgcaa
    10021 attttgaaat ggttgaaaaa gcctataatc ttgcaaaaaa agcgcatgaa ggacaatata
    10081 gaaaatctgg ggaaccatac tttattcatc cattagcagt tgcaaatatt ttaatagaga
    10141 tggagttaga tattgaaact ataacagcag gtttacttca tgatgttgta gaagatacag
    10201 aatataccta tgaagacata aaaaaagaat ttggcaaaga agtagcggat ttggttgatg
    10261 gagtaactaa acttggtcaa ataaaatatc gttcgaaaga agaaactcaa tctgaaaatt
    10321 taagaaaaat gtttttggca atggcgaagg atataagggt tatacttata aaacttgctg
    10381 atagattgca caacatgaga acattaaaat acatgcctcc agaaaaagca aagtataaag
    10441 caaaagagac acttgaaatt tatggtggta tagctcatag attaggaatt tccaagataa
    10501 agtgggaact agaagacaga gcattaagat ttatggaccc agaagggtat tatgatttag
    10561 ttagtagagt atcaatgaag agaagtcaaa gagaggatta tattcaagga attgttgaac
    10621 ttcttaaaga taagtttaaa gatttaagta taccatgtga tgtatatggt agaccaaagc
    10681 atttttacag tatatataga aagatgcaaa aaaaacataa gacttttgaa gagatttttg
    10741 atttaacagc agtaaggata ttggttgata atgtaaagga ttgctatgca gtacttggta
    10801 tagttcatac attatggaga cctattccag gaagatttaa agattatata gcaatgccaa
    10861 aacccaatat gtatcaatca ttacacacca ctgttgtagg tcctgatgga gaacctttag
    10921 agatacaaat aagaactcat gagatgcata atatagctga aaatggtata gcagcccatt
    10981 ggaaatataa agaaggtatc tctagcaaag agtcaaaggt tgaagaaaaa cttcaatggt
    11041 tgagacagat gatggagtgg gaaaaagatt taaaagaccc acaagagttt atggatgcac
    11101 ttaaagaaga tgtatttaat agtcaagttt atgtatttac gccaaaagga gacgtaattg
    11161 aattaccagc agaatcaaca cctatagatt ttgcctatag ggttcataca aatgtaggta
    11221 ataaatgtgt aggagctaaa attgatggaa gaatagttcc tattgattat aagcttcaaa
    11281 atggtaatat tgtcgaggta cttacatcat ctaattctaa tggaccaagt agggattggc
    11341 ttaatatagt gaagacccca aatgcaaaaa gtagaattag acaatttttc aagaaggaaa
    11401 gaagagaaga aaatgttgac cgtggaaata caatattaga aagagaattt aagaagcatg
    11461 atataccaca aaaagaccct cttgttgaaa aatatatggc attagttgct aagaaattta
    11521 atcagccaag tgttgaggac ttaattgcca ctgtgggtta tggtggtata atgtcatctc
    11581 aagtggtatc taaggtaaaa gacctatatt taaaagaggt taaaaaagct aaaaaggaac
    11641 aacagataca agaagaagat ttaaataaac acaatataag tgaaacagaa tacagtaaaa
    11701 agagaaagaa aaacgcatca caaggaatta tagtaaaagg gcttgacaat atcttagtaa
    11761 gatttgctaa gtgttgtaat cctcttccag gagatgaaat tgtaggatat attactaagg
    11821 gtaggggagt agccatacac agagctgatt gcccaaatgc aaacaataat ggtgatttct
    11881 tccaaaatag attggtggag gtttcatgga gttcagcagg aaaaggtaag tttgaagcgg
    11941 aaattcaaat aaaagctatt gacagaagag gaattgttaa tgaaataact catattgttg
    12001 caatggataa acttagttta aacggaataa atgcaagaaa aggtaaggat agtattgtaa
    12061 gtataaactt acttgtagag gttaatgaca cagaggaatt aaaaattcta atgaaaaaat
    12121 taaaagcaat tcctggtgta gaaagtattt atagaatgac aaattagagt taaaataata
    12181 gggaggaaac tatgagagca gtagtgcaaa gagtatcttc atctaaagtt acagtagatg
    12241 aaaacactat aggtcaaata aataaggggt tactagtatt gttaggagtg actcatgatg
    12301 ataaatcaag tgatgtagat tatatgattg ataaaatatt gaatctgaga atttttgaag
    12361 atgaaaatga caagatgaat ttatctctaa tggatatagg tggagagctt ttagtagtat
    12421 ctcaatttac tttatatgga gattgcagaa aaggaagaag acctggattt agtaatgcag
    12481 ctagaccaga acttgcaaat aacctttatg aagaatttgt aaaaaaagca aaagataaag
    12541 gtgtgacagt tggtacaggt caatttgcag ctcatatgat ggtagagtta actaatgatg
    12601 gtccagtaac aatactatta gactcaagta aatcttttta agggggaaaa ctttaatgat
    12661 tataaaaaaa tttgtagata gttattttgg agtaaatact tatgtattag gtgatgaaaa
    12721 gactaaaaaa tgtgcagtta tagacccagg tggaagttta gtagagacat taagttttgt
    12781 aaaagaaaat gaattaaata ttgaatatat tatccttact catgggcatg gagaccatat
    12841 aggatatgtt aaggatatta aagaaaaaac tggtgctaaa gtagttgcac atgttgatga
    12901 aaaagaatta ttaaatgaca aaaataaaaa tctaagttat acaatgagat gtggagcaca
    12961 agagtttgat gcagatatct atgtaaatga taaagacaag cttgaacttg gtgaattaaa
    13021 aatgactttc ttgcatactc caggacatac tcaaggatgt atgtgtataa gagttgaaga
    13081 tgaaatgttc acaggagata ctttgtttgc tggaagtatt ggtagaactg atttatatag
    13141 tggagacttt aaccaaatgg aaaaatcact taagaaatta tgtaaatatg aagatgctgt
    13201 tagagtatat cctggacatg gaccaaccag tactctaggg gttgaaaaga agacaaatcc
    13261 ttacatgtaa ttttatatta gtatttaaaa aagtataaaa tatttaataa ctatattagt
    13321 gagggggttc ttctactgtt aaatagaagg agttaagttg atgttgtatg tttttttaaa
    13381 aggacatgat tataagtatg aagtagcaga acttataaaa ttgtttacca gtgaatttaa
    13441 gtttaaagac tctaatgcta acaataatat agagtcaaag catttaataa atagattgat
    13501 ttatgagaat ggtgtattat tttcaagtac tgaatactat gaaaatggaa acttaaagta
    13561 cgagtcaata cagaatatag aaaatatgaa tcttgaattt ttagatggat ttgatagcag
    13621 caaaacgatt gaagaatttc ttaatagcct agatagtgaa agtaagagga actttaaaaa
    13681 gctgtgtaaa gaaaatatca aaaaaagcat gtttgtagtg cttaaacaag tatttaattc
    13741 ttatgtacca tggggaattt tgactggtat tagaccagtc aaaattgtcc acaatttaat
    13801 ggataaaaag ctagatgata atagcataag aaccattcta aaagataatt attttataat
    13861 agatgagaaa atagatttag ctcttgaaat agctaaaaga gagcgattat ttatgtatcc
    13921 tatagataaa aataaaattt ctctttatgt aagtattcca ttttgtccaa ctagatgtgt
    13981 atattgttca tttccttcaa attcattaaa acaatttgga catttaaaaa gagaatatgt
    14041 ttataaattg attgaagaaa ttaaggggtt tgccaaagtt attaaggaga ctaagaaaga
    14101 agttgaaact ctatatattg gtggtggaac tccaactaca cttgatgagg aagaattaga
    14161 tttgcttata aattcattgt tcaatgaatt agatttaagt aaaattaaag aatttactgt
    14221 agaagcaggt agacctgaca ctattacaga acaaaaacta agagtgttaa aaaaacacaa
    14281 tgttagtaga attagtataa atcctcaaac tatgaatgat gataccttgg taaaaattgg
    14341 aagagagcat agagtatctg atttagtaga ttgctttaat atggctcgta gaatgggatt
    14401 tgataatata aatatggata taattttagg tcttatagat gaagatttaa atatggtaaa
    14461 gaatacattg gaggagataa aaaaactttc tcccgaaagc cttacagttc atacattagc
    14521 cataaagaga gcatccaact taaatataaa tatggatagg tataaagaac atctaacaca
    14581 atatgaagaa atggtaaaaa tgatagactt atcaatgaaa tatgcaaaag atatgggatt
    14641 aaatccatat tatatgtaca gacaaaaaca tatgttggga aatttagaaa atataggtta
    14701 tgctaaagaa ggatatgagt gcatttataa tatacagatt atggaagaaa aacaaagtaa
    14761 ctatgcatta ggtgcaggtg ctatatcgaa atttgtttat gtagatgaag atagaattga
    14821 aagagtagaa aatgtaaaaa atgttgaaca atacattgaa agagtagatg aaatgataaa
    14881 aaggaagaaa gaagaggtat ataaaaatgt tgactaaagc tcctagagga actaaagaca
    14941 taactccaaa agaggcttac aaatggcgtt atgtagaaaa taaatttaga gaaatatgtg
    15001 ctttatatgg atatgaagaa atggtaacac ctatatttga acatacagaa cttttcaaga
    15061 gaagtgttgg agatactaca gatatagttc aaaaagaaat gtattctttt aaagataaag
    15121 gtgatagaga aattacccta aagccagaag gtacagcagg tgtagtaaga gcatttatag
    15181 aaaataaatt atatgcagat actcaaccaa caaaattatt ttatgtgaca ccttgtttta
    15241 gatatgaaag accacaagct ggtagacaaa gacaatttca ccaatttgga atagaagcac
    15301 ttggaagtga tactccttca atggatgctg aaataatcgc actagctgtt caatttttta
    15361 atgaagttgg attaaatgat ttggtagtga gtataaattc agttggttgt ccagtttgta
    15421 gaaaagagta caatgcttta ttaaaagagt atcttgactc aaaagctgat atattgtgtg
    15481 atacttgcaa tgagagaaga gaaaaaaatc caatgagggt tatagactgt aaaaatccta
    15541 catgtaaaga aaatatcaaa gatataccat ttattgcaga ccatttgtgt gatgattgta
    15601 aatctcactt tgataaactt caagaatact taaaggaaat gaatataaac tttgtaattg
    15661 ataaaacaat agttagaggc ttagattact atagaaaaac tgcttttgaa attatatcta
    15721 atgatatagg agctcaaagt actgtatgtg gtggaggaag atatgatggt cttgttgaac
    15781 aattaggagg accaaaaggt ataagtggaa ttgggtttgg actaggaatt gaaagacttt
    15841 tacttacact tgaaggtaat ggaattgaaa tagaaaaccc tcaatctacg gatatattta
    15901 tagtaactat aggtgaaaaa gcaaatacaa gaagctttaa attgttaaaa gacttgagac
    15961 aaaatcatat aagtgcagat aaagaccata tagagagaag tgttaaagca caatttaaat
    16021 attcagataa aataaattca aaatttacaa tagtaattgg agatgatgag cttaaaaatg
    16081 acactgcgac attaaaaaat atgaaaacat cagagcaaac tactgtaaaa ttaagtacat
    16141 tagttgaaga attaaagcaa aagctgtaaa ataagaaagt atgatgtttt gtgtattaga
    16201 taaatatata gcattaaaat taaataaaat ggaggtagta tcttggaaac tttaaagggt
    16261 ttaaagagaa ctcactactg tggagagttg agagaaaaaa acataaatga agaagtcgta
    16321 cttatgggat gggtacagaa aaaaagaaat ttaggtggtc ttgtatttgt tgatttaaga
    16381 gatacaagtg gattatgtca aatagttttt gatacagatg tagataaaga agcttttgaa
    16441 aaagctgaaa aattaggggc tgaatttgta atagctgtaa aaggaaaagt atgcgaaaga
    16501 caatctaaaa accctaatat gccaactggt gatatagaaa tatttgcaac tgaacttaga
    16561 cttttaaaca agtcagaaac tccgcctatt tatataaaag atgatgatga tgtttctgaa
    16621 gcactaagat taaaatatag atacttagat ttaagaaaac catctatgca aagaaattta
    16681 aaattaagac ataaagtaat gaatattaca agaaattact tatcaaacaa tagattttgc
    16741 gaaatagaaa caccattttt aatagctcca acaccagaag gggcaagaga ttatctagta
    16801 ccaagtagag ttaatccagg aaaattttac gcattaccac aatcaccaca attatataaa
    16861 caattattga tggtaagtgg tatggataga tatttccaaa tagttaaatg ctttagagat
    16921 gaagatttaa gagcagatag gcaaccagag tttacacaaa tagactgtga aatgtctttt
    16981 gttgagcaag aagatgttat gagtatgatt gaaggtttgc ttgaagcgat atttaaagaa
    17041 gttttagatg tagaattggc acttccactt cctaagatga cttatgcaga agctatgtct
    17101 aaatatggtt cagataaacc agatactaga tttggatatg agttaacaga tatatctgat
    17161 gttgtttgta actgtggatt taaagtgttt gctgatgcta cacaaccagg aaaaagtgtt
    17221 agaggtataa acgtaaaagg caaagctgac gactttacta gaaaacaaat atcatcacta
    17281 gaagaacatg ctaagacata tagagcaaaa ggtcttgctt ggatgaaagt aggacaagaa
    17341 ggagtaactt ctccaatagc aaaattcttt aatgaagaag aaatgaacgc tatacttaca
    17401 agaatgaatg ctgaagtagg agatttactt ttatttgtag ctgataaaaa ctcaatagta
    17461 tttgatgctt taggacaagt tagacttgaa gtagcaaata ggcttaatct attggataaa
    17521 aatgtatata acttactatg ggtaactgag ttcccagttt ttgaagaaga tgaagaaaca
    17581 ggcacattct cagcaatgca tcacccattt acatcaccta tggatgaaga tttagacaag
    17641 ttagaagaag gtgataagtc atctttaaga gcgaaagctt atgatattgt attgaatggt
    17701 tatgaaatag gtggaggaag tgttagaata tcaaattctg atgttcaatc aagaatgttt
    17761 aaagcacttg gttttacaga ggaaagagct aatgaaaagt ttggttattt attagaagca
    17821 tttaaatatg gtactcctcc tcatgctggt ctagcttttg gtcttgatag acttgttatg
    17881 ttacttgcag gaactgataa tataagagaa gttattgcat tccctaaaaa tcaaaatgct
    17941 gtttgcccta tgacaaatgc tcctacattg gcggaagatg agcaattaga agaattaagt
    18001 ataaaagttg atataaaaga taatgaataa tatgtagttg caagaaacta aaatgtaaaa
    18061 agtctggata taaagtatat ttatattcag acttttttta ttagaattat tttatatttt
    18121 ttctctatat attttcataa attatacttg tattctcaac caatataatt aaaatactat
    18181 gtctaaaaat tttacataaa cttggttgtt ttagaaacta cttctgtaat gagtacacga
    18241 ttttaattaa agaatatata acaaatttga aataagtcta atatatgact taatagatta
    18301 aattaaaata ataaatttgg actatttatc tatttataac ttaaattata tcaaattgat
    18361 aacaaactag atattatact tttttgtaga atagaattac aattatataa agaaaaattc
    18421 tattaatagt gaaaaaaaag tagaaaaaag tagaaattat tcttttataa agaaagggag
    18481 ggataatatt gcaagagaga gaagaattag ttttaaatcc agttccaaaa gaggaaagag
    18541 ttggatggtt agctccattg tttaatatgc ttggttctaa catagctata tctgaactaa
    18601 tggttggggg aactttaatt ttaggaatga ctctttctaa tatgattatt acatcaatta
    18661 tagggaactt aatattagtt gcaattatta tgatacaagg ttatataggt tacaaagaag
    18721 ggttaaatac atatgtatta gctaaggggg catttggaga gataggagga aagtatctta
    18781 tatctctatt acttggtata actagttttg gatggtttgg tgtacaagca ggggttgcag
    18841 gactatcagt acaaaagata ttcccaagtg taaacctaac attagttaca gttatacttg
    18901 gattatttat ggttgttttt gcactgtatg gctttaaagc tatggctaaa tttaattact
    18961 tagttatacc accacttatg atattaatgg tgtggggagt ttttaaagct ttttctactt
    19021 atggagttga agctatttat aattatactc ctcaaacaac tatgagtatg gttgaaggtc
    19081 ttaatattgt agtaggtctt gttatagttg gtgcaattat atcacctgac cagttaagat
    19141 atacgagacg agtaaaagac atttggatta tcagtttttt aggtctaggg attatttcat
    19201 tattccaaca agttgcagct ggtgtaatgt ctatgggagc acctacttgg gatataacag
    19261 aagtattggc taatttaggt tttggatggg tagcgtttgt tattttaata cttgcatctt
    19321 ggtctacaaa cgtatcaaat gcttattctg gtggattagc attaaaaact atttttccaa
    19381 atgtaaaaag aaatgttttg actttagttg caggtctaat aggtacaata atagcagcta
    19441 ctggtataat atttaaattt cagtcatttt tatctttctt gggaatcgca gttcctgcaa
    19501 tagcaggaat tatgtggtgt gagtattatt ttatacaagg aagaacttat aagcatagag
    19561 aaggaataaa ttggattgca gtaataagct ggttaatagg ttttgcagcg tcgtattata
    19621 gttcaaaaat aaatttctta atacctccga taaatggaat tgtagtgagt atggttattt
    19681 attatatatt aatgaattgt tttggaatca aagataaata aagggggcaa ttacatgggt
    19741 cgtaaggtga aaataggtat aattcaacaa cacagtgtac ttggaaatgt taaaaaaaat
    19801 atagaaaaag cagtagagat gatagatgac ttaggaaaac aaggggcaga tataatttgt
    19861 ttaccagaac tatttgctac aggatataat ttagagtctt taggtggagt taaaacatta
    19921 gaattaataa gagaacataa taagtacata gaggaatcaa tgtcagaagc tgctaaaaga
    19981 aataatgtat acctgatatc tccatatgga accttagaaa aagggtctac acatgtttat
    20041 aattcagcag ttatatttga tagaaaaggt aagattatgg gagaatactg taaaaatcat
    20101 ctgtggtcat tagaagcagt atactttaaa ggaggggaaa aagtagaggt atatgatgct
    20161 gattttggta gatttggtgt aatgatatgt tatgatgcag gttttccaga agtatccaga
    20221 gaattgacat taaaaggttc tgaaataata tttataccat ctgcatggag aatacaagat
    20281 gaagatatgt gggatttaaa tgtatctcaa agagctttag agaacactgt atatacagta
    20341 ggtgtaaatt tagtaagtaa tgactctaat ttaatactat ttggaaagag taaaatatgc
    20401 aatcctagag gtactgtaat aactcaatta gatacataca gggaaaaata tgtattatca
    20461 gagattgatt tagatgaaat aaaaaagtat agggaagata tcccctattt gaaagacaga
    20521 aaaagtagtt ataacatata attctatact ataatacaag ttcaatataa aatgataaaa
    20581 agaggatgta tcaatattaa gattaaattt attatacttt gatatatcct ctttaatatt
    20641 atcaactttg atttaaatat ttttaaaata gttgataaga aagtttttga acaacaacac
    20701 agctttagat tcatagtttt cgctaaacca tgaaaggttt aaatatcttt taaattcaat
    20761 atctttgata tgtaacaact taatctcagg gtcttgagat aatccccatg attttccaca
    20821 tataaatcca actccttgaa gtgattttat aagagcataa attgtatatg gtgaatcaga
    20881 ctcaaatgct attttgggtt tgaaattagc tgattcgcac aggatatcaa ttacctctct
    20941 ataattctca cccttcgtta taactataaa gttttcatct gaaacttcgc ttagatatac
    21001 ttcatccttt aatgataaag gatggtttat tgaaactcct aatagtattt cttcgcatgt
    21061 taaagttatg ctattttctg agtttagctt tgtaaatgaa gaagatatgt acaaatcaaa
    21121 atcacttttt ttataagatg atggcaaagt atgtgacaca ttaaaagtaa tattgggata
    21181 tagttttctg aaattactca aaagtgatgg taacacatta gccgctgcag gagataatat
    21241 ctttacttct cctgaatttt ccaaagaagt gtcttgtaac tccttttttg aactttctac
    21301 taaatctaaa atgctatcta ctctttttag aaacaatctt ccatactcat ttaaaacaat
    21361 atatcttcct tttctgtcaa ataactgaac tccaagttct ttttctaagg aagatatagt
    21421 tttgcttaaa gctggctgtg ctatatttaa cttgtgtgat gcttttgtta tgtgctccat
    21481 tctagcaaca gtttgaaaat acttaagatg caataaatcc atagtataaa atacacctct
    21541 tttaatatga cttgagttat atcaatttta taatcaactc cacattatac attataatat
    21601 aaagtttata taataaacta caatatatta aaaagttatt taaattgtta tttatatttt
    21661 gtacataatc taattaaaaa aagtaacttt aaatgattaa ctaaggaaat gttgttacaa
    21721 actttagtta tataaaaaat tcatttgatg agcaaagtac taatatcaag taagaataaa
    21781 agcttattag ataagaaaaa taatatttta taataatgat gttacttaaa ttattatgtt
    21841 atactatttt aaataggaaa aatttagata ctataatttt agggggagtc aagatgaaat
    21901 taactaaata aatatcatca atgctgtgct tatctctagt tttaagctta tcaccagtag
    21961 catatgcaaa tgaaataagt agaacaacag ttgagaatac tttagtaggt caggatagat
    22021 atcaaactgc tgttgaagta agtaaaaaag gatggagtag tgcaaatgag gctgtagttg
    22081 taaatagtga atctattgta gatgcattag ctgtaacacc atttgctaaa ttaaaaaatg
    22141 ctcctatatt attgacagaa aaaaataatc taaattcaca aactgagaaa gaattaaaaa
    22201 gattaggagt taaaaaagtt tatataattg gtctatctag ttctgtatca aatgatgtgc
    22261 aaaaacagtt agaatctaat aatttaacta ctgatagagt tggtggtagt gatagatacc
    22321 aaacagcttt gagtatagct aaaaaaatag aaggattaaa agatatatca gaaatagctg
    22381 tagtaaatgg gtacacaggt cttgcagatg cagttagtat agcttctgta gctgctacta
    22441 atggaatggt tatacttcca gtatctgatg catctggtat atctagcttt aaagatttta
    22501 tagaatctaa gaatataaat aaatcataca taataggctc tactaatgct gtatcagata
    22561 aaataaaaca aagtttacct aattcagaaa gaataggtgg agcagataga aatgaaacta
    22621 atggtaaagt aatagaaaag ttttatacgt ctaataaatt aaacaatgta tttgtggcta
    22681 aggatggaat gaaaaatcaa aatcaactta tagacgctct agcagtaggt gtactagcat
    22741 caaaagaaaa ttctcctgta ctaatcgtag gagaacaatt aagtgcaaca caaaagagtg
    22801 tattttcgaa taaaaatgct acctctataa ctcaagttgg tagtggagga aatgaaaatg
    22861 catttaaaga attaaaagat ttacaggcaa atagtaacaa taagagagta atgtatgtaa
    22921 ctaatacaga taaggtaaat ataagaaatg atgctacaat agaagcttct gttataggat
    22981 atctaaataa tggtgatgaa gtagaagttt tagatgtgtt aaaaactgga tgggtaaaaa
    23041 taaaatacaa tgaaggtatt ggatatgtta gtgggtctta tttgacaaat aataagcctg
    23101 ataatagtaa tgaaaatata aaaataaagt atgtaaaaga aaaagatggt ctaaatgtta
    23161 ggaaaggacc aagtacagaa gatgaaaaga taggtcacct ttcatatgga agcaaagtag
    23221 aaacaataga aatgtttgct actggatggg taaaaataaa gtataatggt ggatatggtt
    23281 atgtaagtaa tgattactta tctgatacac cagttattta attataagta acaataaaaa
    23341 aactgcttta atttgtaaag cagttttttt ataaaaattt aagtctaata actatttcta
    23401 ttttgatacc ttaatcctgg gatttattat tatggatatt atcaacatac aaacactcca
    23461 aatccagtgt ctaattccaa acttagatat taataaattc agtcctgcta tggtaaataa
    23521 gcagtattta attacaatta aaagtgtatt tatagaaagc cccctcatag aaagtatcct
    23581 aaatccaata atagacattc taagtcctat aaatgccaat aatatacctg caatatcctg
    23641 aattaagtaa tagtattcaa atttcatatt caatcaactc ttctctgagc agtatcaaat
    23701 atttctaact cttcaatttc atcatctgtg tatgtaagca tagtaaaacc agttacacca
    23761 taaataaatg aaattatagg tgtaatccaa cttaagaaac aatatggaat aaattctagt
    23821 ggacttacac caagagtctg agcacagaaa acagcatttg aattccatgg tattaatgga
    23881 ccaccaagtg tagctgtatc ttctataatt ctagatagat tttgtggttt taatttaaat
    23941 tctttgtata ttggagacat tagagttccg acaaatacag aagtgaacat cattgatgaa
    24001 ctaaatgcat ttgcaaagta gcttactata aaagtagacc caaccaattt agttcttgat
    24061 tttcctattt tagttataag aggttgaagc agtacttcta agaatccagt tttttggaga
    24121 ataccagcaa taacgaaaac tacaaatact agtaaaacgg tttcagccat actcattatt
    24181 ccaccacgat ttaatagttt gtcggcatag acaaatccag tatctatagt aaatcctgat
    24241 agcatgtaat ttaaaacatc acctattttt tctccttctt gaaatactgc tataataagt
    24301 cccatgatag ctgaacttag tatagatata atgggtggct tttgtagcaa taaaagtaat
    24361 aataaaaata ttattggaat cattgctact aaaccaatat gaaagtttga atttaataca
    24421 tccttgattt gatttatttg aacatagtct atagtattat ttgaatactt aaatccaatt
    24481 actgtataca gaactataca tattacgtat gcaggtcctg tggtataaaa catatgcttc
    24541 atatgtgtaa ttacatctgt cttacaaact gctgcagcta agtttgtaga atctgataga
    24601 ggtgacatct tatctccaaa gaaagcacca gaaatgactg caccagctgt taaaccaaga
    24661 ggaataccca tactagtacc aatactcatc attgctatac cagaagttcc aagagtcccc
    24721 caagatgtac cagtagcaac agaagttaat gaacatacga ttaatgttgt aagcagaaaa
    24781 tatttagggg ttattattgt tagtccacta tatactactg ttggtattgt accagcagct
    24841 atccaagctg ctattagtac accaacagaa agtataatta agtttgattg aaaggcatct
    24901 ttgccaactt caaatccaaa agcttctatt tcatcattag tataaccaag tttcatggct
    24961 gcaggaacta ctataagcca tgaaagtaaa aacattgtag ttatagaagc attaaataca
    25021 actataccaa ctgatgttag agctattatt gcaagcatta ctaacaagga atatctaaat
    25081 gaaggtattt ttctttctgt attcattttc atagtgcaaa ttctccttaa atatttttag
    25141 ttaattgcat tttttattct tataagagct tcttcaagca tacttcttgg acaagctaag
    25201 tttattcttt gatacccact tcctccaatg ccaaagctgt ttccttgatt tagagcaact
    25261 ttaccttttt gtacaagtat actttctagt tcctcatcac ttaatccaag tgcactaaag
    25321 tctacccata ataaataagt accttctggt tttctaactt taagttttgg catattttca
    25381 tttatatatt ttatagcaaa atctatatta gattctaaat attctaaaaa tgattctagc
    25441 caagattcac cattattata tgaagcttca gttgcaacta agctaaagca attatttctc
    25501 tttatatcaa ttctggtaaa ggcatcatct aacaatttat agtatttttc atcaggtagt
    25561 attacatatg atgattgaag accagctata ttaaaagttt tagttggagc catacaagta
    25621 attgtatttt tttcaaattc tttagaaata gatgccatag gaatatgctt atgttttttt
    25681 aatataatgt cactatgtat ttcatcagat atgattttta cattatgctt aagacaaata
    25741 tctcctagtt tttttaattc atcttttgtc catactctac caactggatt atgaggatta
    25801 caaagtatga ataattttac atctttaatc ttgttttcaa tgtcttcata atccattatg
    25861 tagtttccat tttctaattt ttgtaatggg cttataatta gttctctatt gttatttttt
    25921 acaacactat taaaagggct gtaaactggt tcttgtatca ttattttatc atttgctttt
    25981 gtgagttcat ttattaaaag acttatagct gggataacac ctggactata aattaaccac
    26041 tcacttttta ttttccagtt atgtctccta tctagccagt ttactataga ttcattgtaa
    26101 gaatctggtc ttgttgtgta gccataaatc tcttgttcta gtctattttt taaagaatca
    26161 attatacaag gtgcagcctt aaaatccatg tcagccaccc acataggaag taaatcatta
    26221 gttccatact ttttttccat ttctgaccat ttagatgaaa aattattgct tctatcaaca
    26281 atctcattaa aattataatt cattgtaaat acctccagta attttaattt tatgttaata
    26341 atattaagca aacaacgtgc caatattgat aataaaaaat atttattcac aagttaatat
    26401 ttaatattaa gtaatttcag agcatagata agataaaaat attaattata cttaaaactt
    26461 tgtggaaaag gttatttcta ataaattttt aaatatatga aaatgtaatt aaataggtta
    26521 aataggttta actgtttaac ttgttagacc tatttatatt ttttagaaat tctactccct
    26581 ttgatgttat aattgaacct ccacgaccaa caagtatgtc tacaagccca aagtcttgta
    26641 gttctaaaag catatttcta atctgttgtt ctgtcaaaaa tctattttcc tctaaagcta
    26701 ttttacataa gctttttcta cctattcttt cttttaaatc ataagccttt tttaaattat
    26761 ccaatataaa tatgtattca tctagtgacc ttttaaaatt atagtcgtat ttcatgatgt
    26821 ttttgtcttt gccaatgttg aattgatact tttttatatt attacttact ttattacaaa
    26881 cggttctact ataatttgaa tctatagtat ccaatatata ttcaggtaaa tcacatattt
    26941 ctataatatc tttttccaag taacagaaat attctcctaa gtttcttaat tctcttacat
    27001 ttccttccca tctatacatt ttaaagattt cttttacttc ctcacttaga ataaaattac
    27061 atggaatatc atattttaat gaaccaaaaa tttcaaatat atcagcaggt ctttccctaa
    27121 gtggatgtat tttaagggga agaacattca acctaaaata taaatctctt ctaaacatat
    27181 ttttagatac aagttctttt aaatttctat tagtagctgc aattattctt acatcaatgt
    27241 caatgacatt attagaacca atacgtcgta cttgtttttc ttgtatgact ctaagtaatt
    27301 tagattgaga atttaaatcc atttctccaa tttcgtctaa aaatatagtt ccattgttgg
    27361 ctaattcaaa taaaccaatt ttcccacctt ttttagcacc agtaaaagcc ccttcatcat
    27421 agccaaataa ttcactttgc agtagatttt cttgaaaagt actacaattt actgcgacaa
    27481 aaggaccatc ttttctccta gaagcactat gaatggattg agcaaataat tctttaccag
    27541 taccactttc accaattata aggatagatg aatttgattg agccatttta ttagcaattt
    27601 tttttgtatt aatagtttgt atactagaac ctaatatatc atcaaatgtg tatttactga
    27661 tattaccaga gtttactagt tgtgctctta atttagcttg ctttttttct aattgagaaa
    27721 actttgtcat ctttagtata aatccatcaa atacatttaa ttgtatatgt ttaatctcta
    27781 aatttatatc aatatgattt attttaatca atttctcaaa aaatggagtt ttattggtca
    27841 atatgctttg aaaattgatg tcttttatgc aatcaccaac aaatgagtca atcatttcat
    27901 ttgcatttat agacaaaatt tttctagcca tatgattata gaattgaatt attccatcgt
    27961 tatttgtgca tataattcca tcatcaatta tagatagtaa caaatcaaat tgtctttcaa
    28021 acttatttgc atctaacaat aactcacctg tactataact tgtaggaatt attttttcta
    28081 cgtatttttt tattaggtca tcttttatta gatgttttaa tttggttttt gtagcaattt
    28141 caacaataca acttaaatct attattctgt attttaaatc tataacttca caatttttaa
    28201 acttaggcaa gattttttcg ccaggagtta ggattacagc atctgtggga gtaaactcaa
    28261 tctctggata gcagggcagg atatttatat tgtctattcc taatctatgt attaaagaaa
    28321 ttgtttctat tgccatatct ttacttaagt tatatacata aataatttta tctttttcta
    28381 atttggatat catatcaata ctgttctttt caaatgtcaa cttgggaaca atgatttgag
    28441 catcatagtg agataatttt actatttcat catattttaa ataagcagat aggagtataa
    28501 gtttttcttt aaaaaacttt aaatttccct cttcaaatga gtataaattt atctttagat
    28561 tatcacctaa gaattgcctt atttgatttt cataaatttc tccagcgtct ttttttaaac
    28621 ttacaatagc taattccatt tgtttcattt taacctctca tacaaatttg acttatttta
    28681 gattacataa taattaaatt agattaaata atattattta attattatta taaatttatt
    28741 atagactaca cattgaagta aaagaagtag ctaaaattat tccttttatt tttgtttttt
    28801 gtatctattt ggtataatag tattagagcg tataataatt taggcaaaat tttttaagga
    28861 agactagagg agaatataaa atgaaaaaat gtctttactg taatagggat ataaagtcac
    28921 caaataataa attagctata aaatatgatg acaatatgaa tatatatcca tgtcgtgcag
    28981 aatgtaagga aaaaattgaa gaatatatag caaaaaaacc tacatataag tttataaata
    29041 tattagaggt tcttttaggt gtgggcggaa tattctgctt tttgagtaaa tggactattg
    29101 cagcccaagt tgtacttgga attgatgcac tggttatatt tttattccca ttggcaggat
    29161 ttaagatgaa tggaaagtta agtatggaac aaactaagaa tcaaagtagg tctattgcaa
    29221 tatcaatttt ggcatttata gtaataataa ctgtattata ttttaaacaa gttggtaagt
    29281 agagcataat tgtataagac atcattagag tatgaggtga gtttatggaa gaaagaaaat
    29341 tagcaccaaa caatgagagg gaagctctca taaaaagatt aaaaagaata gaaggtcaag
    29401 ttaagggtat acaaaagatg gtggaacaag aacgttattg tgttgatata ttggttcaaa
    29461 tatctgctat aaggtctgct ataaatagag taggcacaat tatcttagag aatcatataa
    29521 aaggatgtgt agcagaaagc attaaacatg atgatgctga taaaactgaa gagatgataa
    29581 aagagcttat ggatactata aataaattta caaaataagg aggcggttag ttatgaatca
    29641 acaaggattt attatggaaa ttgttgatga tagaactgca aagttaaaaa tgcaaagaca
    29701 ttcagcttgt gctgcttgtg gaaaatgtgc tactacaaat tcagcagagt caaaagaaat
    29761 attagtagaa gtagacaaca caattggtgc aaaagttggt gaccatgtag aagtgagtat
    29821 ggataatatg aatgtattaa aagcagctgt aatggcttat ataatacctc ttatagcact
    29881 tcttgtagga acaataggga cttattatat attgggggca attggaatga caactggagt
    29941 tgaagccata agtggattag ttggaatttg tcttacaata atctgttatt tatacttaaa
    30001 gaagaatgac aaaaaattta gagacagtag agaatttata ccagtaataa caagaatatt
    30061 aattgacctt taagataatt ataattaaaa aatagcaatg tactttttaa ataaatactc
    30121 aatacgtatt tgtagagttt aagtacattg ctatttttat atttttattt tgattttctg
    30181 taaggaataa agttttttct gattttcaaa agattcaatg ttcttacaaa agaagttaca
    30241 actaatacac caagtgctat tgcagcactt agaaatagcg catctgtacc ttttgacata
    30301 gccaaagaat aatttttctg aatcaaagac aacattgtat tatatagccc aattcctggt
    30361 attagaggaa gaatacctgg aattacgaac acaatagctg gttgctttag ttttcttgct
    30421 aaagtctcac tgcacgctga gaccaaagca gcagcgataa aacctgcaaa aactgcattt
    30481 gtagtaaaat taaataaata aacatataca atccaaccaa ttccgccagt tactccagct
    30541 ggaagaagta gatatagtgg agcattaaag aaaacagcaa atccagcagt cgcaacacct
    30601 gaaaataaaa aatgcaaaat catagacata tcagtcatta gaaaggacct cctattctat
    30661 accatgtatc aaggacaaaa ccaactccag aagcaattgc aataataatc atagcagcat
    30721 caaaagcacg agaaattcct gatattaaat tccctgatat taaatctcta atagctttta
    30781 taaaagcaac tcctgggagt agtggcatta tagcgccgac tataagcatt ttagggtcat
    30841 ctaaaatgcc tatttcaact agtagaactc ctgaaagtgc aattaaaaaa gaagatacta
    30901 aacaggaaaa tgcagatatt gcacttatct tcatcgtttt atcataagta attgcagcaa
    30961 agattgctat tataaacgta agtataaaat ttaatacata attccctcca agcaaacaag
    31021 caaaacaagc agatgcaatt cctgtacaag agtaaaataa ccataaagga taaggtttta
    31081 catcttggat ttcataaaga cgtgcaatgg cattatcaat atcatactct ttgtcactaa
    31141 caaattctcg agaaaaactg tttaataaag aaatcttatt taagtttata cctcttgatt
    31201 gaatcgtttt cataaacgaa aagccatcaa atttctcatc tgaaataatt attactgtag
    31261 gagttgtaaa aatatttaca tgataaaacc ctcttgacct gcatattcta aggacactgt
    31321 cttcaaccct agatgtttca gctccatttt ttatcatcag ttcccctaca aatagagcta
    31381 gttttagaat atttttctta tattcaagtt cctcattttg attcataaat acacctccaa
    31441 attaaaaaac gtaatattga aattatatca aatttttaag taatattcct taatatctat
    31501 ataaatatat tatctttttt ttgtttcaaa atagtcacat ttaaattata tccaattaaa
    31561 agaattagac aatattattt attatatttt aacttaaaaa taaagaaagt aactatgtta
    31621 aatatggaaa aatggcaaaa cacttgacta ttaattggta aaggactata ataataaatt
    31681 atcatattga taaaaacgaa aattatgaaa tgtctgtaaa aataaaattt aaataataac
    31741 taggaggagt aatatgtttg agaacttgaa aaaagcggac cctgagattt atgaaagtat
    31801 gaaaagagag ttaaaaagac aacaaagaaa tattgaatta atagcatctg aaaacatagt
    31861 atcagttcca gtaatggaaa ctatgggaag tcatcttaca aataagtatg cagaaggtta
    31921 ctcaggcaaa agatattatg gtggatgtga atacatagat gaagttgaaa ctcttgcaat
    31981 agatagaatc aaaaaaatat ttggagcaga acatgctaat gttcaacctc actctggagc
    32041 aaatgcaaat attggtgtat attttgctat gttaaaacca ggtgatgttg ttatgggaat
    32101 gaatttatct caaggtggac atttaactca tggtgctcca gttaatattt ctggtcaata
    32161 ttataaattt tatgaatatg gtatagataa agacagtgga cttatagatt ttgatgaagt
    32221 tagaaagata gcacatgaag taaaacctaa aatgatagtt gcaggagcaa gtgcatatcc
    32281 tagagaaata gattttaaaa agtttagaga gatagctgat gaggttggag cattgcttat
    32341 ggtagatatg gctcatatag caggtcttgt ggctgctggt cttcaccaaa atccatgtga
    32401 agtggctgat tttgttacaa caacaactca caaaacttta agaggacctc gtgggggtgt
    32461 tattctttgt aaagaaaaat atgctaaaga catagacaaa gcaatattcc ctggtattca
    32521 aggtggacct cttgaacata taattgcttc taaggcagtt tgctttaaag aagctttaag
    32581 tgatgagttt aaagaatatc aggtacaagt tgctaaaaat gcaaaagcct tagctgaaga
    32641 acttattaaa agagatttta aattgatttc tggaggtaca gataatcatt taatactatt
    32701 agatttgact aataaaaata taactggtaa agcagcagaa aagagattgg atgatgctta
    32761 tataacagct aataaaaata caattccatt tgacccaaat ggtgctcttg ttacaagtgg
    32821 aataagactt ggtactccag ctgtaactac tagaggtatg aaagaagaag atatggcaat
    32881 aatcgctgag gctatagacc tatgtttaac ttatgatgaa gaatctaagg ctagaacatt
    32941 ggttgttggt ttaactgaaa aatatccttt atatgaagaa tataatatta tggattaatt
    33001 ataaagaaca actaaataaa atacatttaa ataattacaa tgattgtgta tgtatttgaa
    33061 tatatttaga tataaaaaag gtgtctcaaa atgaactttt tagtttatga ggcacttttt
    33121 tgtatggaat cgaacatatt tgtattattt aaataataaa aaatatggtt atctctattt
    33181 ttagatggtc tctttgtatt ttttaattaa aactttaaga tttcttaaga attatactag
    33241 gtttttctaa agatttaaag tctatactta taagtatagt aatcatatga aaagaaaagc
    33301 tttaacgagg agggaatctt atgaaggtat taattcttac aggaaaattt ggtatgggtc
    33361 attactcagc atcaaattct ttaagtgaag atataaaagc aaaatttgat aattcagaaa
    33421 taataattaa agatatattt gagtacatta tgcctaacta ttcagataaa atgtacaaga
    33481 ctttttctat tcttgtaaat cggggaagta gtctttataa tttgttttat aaatgtgctg
    33541 aaaatggaaa gaaagatata aaatttactt tttcagatta ttttttaaat aaattagata
    33601 cattacttca tgaagtacaa ccaacagtag ttatttcaac ttttccattt tgttctcaat
    33661 tagtatcaag atataaggaa aaatataatt caaatcttcc tttaataaca tgcataactg
    33721 atataagtag tcactcagaa tggattagta aaaatacaga ctgttattta gtagctagta
    33781 aatctactaa ggaagaactt gtttttaaag gaatagatga atctaagatt aaagttaatg
    33841 gtattccagt aaaaaaagaa tttaaaagaa tagaacatgt aaatcattca actaaaaaaa
    33901 atattcttat aatgggtgga ggattaggat tactgcctaa atcagaacaa ttttacaaag
    33961 aattaaatag tttagaagga gtaaaaacta ctgtaataac tggtaataat aaaaaaatgt
    34021 actataaact atatggtaga tatgaaaata tagaggtagt aggttacacc aatgaagtat
    34081 ataaatatat gaaagattct gaccttatta tttctaagcc aggtggaata acactatttg
    34141 aaacaattta ttcagaactt ccaatattgg catttaatcc atttttacaa caagagatag
    34201 ataatgctag ttttatatta aataatgaaa ttgggagaat acttggtaaa aataaaaaat
    34261 actatgtaga tgagataaaa gatttaattt atgatgatgc tactcttaaa gaaatgagca
    34321 gtaacatgaa agaattaaaa aaacaatttg ataataatac cttagagaat attttatttt
    34381 cacttgatga acaaggagcg tgtagagaat gtatgtagta ggtttaatta tcgttgttgc
    34441 attgattttt ttagtccatt caattatacc tacatactat aataaactat taaataaaga
    34501 ggtattaaaa aatatggctg gagaaaatga aattgcactt acatttgatg atggtccaga
    34561 taagagatat acagaaaaat tactagatgt gttaaaagaa aataatatac aagctatgtt
    34621 ttttgtagta gcaaagaatg ctgaaaaaga gcctgaaatt ataaaaagaa tgttgagaga
    34681 aaatcatata gttggtttac attcattgga acacagaaat gcatggcttt atagttatag
    34741 ttatgttaaa aaagatttta ttgaaagtac aaatataatg aaaaatttag gtgtagatgt
    34801 aaactactat cgtccaccat ggggtcatac taatatattt tcaaattcat ttgttaaaaa
    34861 atataatcta aaaatgactt tgtgggatgt aatggcagaa gattgggaga aagatagtac
    34921 agtagacatt attataaaca agttaatgag tagaacaaaa gaaaattcta taatttgctt
    34981 acatgatgca ggtgaaaatt caggtggtgc agtaggtgca cctgagcgta ctattgaagc
    35041 tttaaagata gctattccaa aacttaaagc cagtggttta aagttcgtaa caccagaaag
    35101 gatgtagtta tgataagtaa aactgaaaag aaaaataaat gcctaggtag tgtagctttt
    35161 ttagttttac taatgggcat aacaggatat tttgttttta gagggcagtc agttgaatca
    35221 ttgataaaat cattaaaagg tgcaagccct atgtttatac taattggatt tgcaatgatg
    35281 tttatatatg ttgcctgtga aggtataaat atatacttag gtatgaaagc tttaaaccaa
    35341 aagactactc ttttaaaatg tatgggatat gcttttattg gattttattt tagttctatt
    35401 acgccttcag catcaggtgg tcaaccagca caagtttatt atatgaaaaa agacgatata
    35461 aatatatcat attcttcact tatactatta gtcattgttg ttattcacca agtagttata
    35521 ctggcatata gtggaataat gttcattatg gaacgtgagt ttatattaaa taatgtaagt
    35581 ggtatgaaca ttttactaat atatggtgta ataacaaatg ttgctcttgt cattggagta
    35641 attgcaatta tattttcaaa aaaacttgta aacaatttta taatatcaat aacaaattta
    35701 ttaggtaagt taagaataat aaaagatgtt gaaagttcaa gaaaagttat aatatctcaa
    35761 attgaagagt atgttaaagg tgctcaatat ataaagcaga atccaaaact tgtagtccaa
    35821 attttagtca taactattgt acaaataaca gctatgtttt tagttccttt ctttgtatac
    35881 aaagcttttc atctatcaac atatactgta tttgagatat tagcgataca atccttgctt
    35941 aatattgctg tttcttcatt accattgcca ggggctgttg gagcttctga aaatagtttt
    36001 atgacattat ttaagatatt tttccctggt cacttattag ttccggcaat gttacttagt
    36061 aggggaataa gtttttatgc atttgtagct ataagtggat tgatatgtat agtagtacat
    36121 gtaaaatctt ctgaaaaagt aaaagcagtg aaaaaagttg cttatgtaag ataatattat
    36181 atagattatt tttattaata tgtcaatttg tgtgtatatt ttaattaagc tttaaacatg
    36241 tttacaggag tagaaactat taaaacgtgt gacattgcta agcttttgct aataaagtat
    36301 aatattttat atagtttgaa atatttaaga aatatagcaa aaaaaggagc ttggtgatga
    36361 ataaagagat tccaaaaaaa attttagtag ctgatgataa tcctgagatt agggaaatcg
    36421 tagaaatact tttaactggt gaaggttatg aagttattat ggcaacgaat ggagaagaag
    36481 ctgtaaactt agtagatagt acgattgatt tgattatttt ggatgttgta atgcctgtaa
    36541 aaacaggatt tgttgcatgt aatgaaatta gagaaaaaac gacagcacca atattatttt
    36601 taactgcaaa aactcaagac tctgataaag ttattggatt ttcagcagga ggagatgact
    36661 atttatcaaa gcctttttcg tattctgaac taatctcaag ggttaagtcg ctacttagac
    36721 gttattatat ataccaggga aaggaaaagg aaagagagcc aaatatatta aaaacaaaag
    36781 agttatgtgt caatttagat actcaagatg ttacactaaa tggagaacct gtgtttttga
    36841 caactataga atatagtata ttaattctta tgttgaaaaa tagaaaaaaa gttttttcgt
    36901 ctgaaaattt atatgaaact atttgggatg agcagtattt ttatacggca aataatacaa
    36961 taatggttca tattagaaat ttaagaaaaa agttagaaaa agaccaaaaa aatccacaat
    37021 atataaaaac cgcatgggga aaggggtatt atattgatta acaaaattaa gccatctaag
    37081 ttgggagtta tactattgat atatgtagta atctcagctg cagtggcttt cattttaaat
    37141 ataccatttt actctgctgt tgaatctata gtaaaagatg tatcagaagg gaaagtatat
    37201 tttaaaagag aagtaaatca acttatggat aattttcaaa tttatgtgaa taacaatggt
    37261 ataagagcag atgatggaga tgatatagca aaatggaata gtgataattg gtttgtttct
    37321 atgaagatat ataaaggaaa taaggttttt tatgatacat tatactatcc taatgaagta
    37381 cctgataaga gtgaggaaaa tgagttttat agcagtccca tggctagaaa ctatgagata
    37441 caattttctg atattaaagc taatatagtt gtagtagctt tatttagatt tagatttcaa
    37501 aattttatag aagcaatttc tattacattt atggcactta catttattat aacattttta
    37561 ctgttgttta gtagaaagat aaagtaccta aggaaaatag aaatgggtat aaagatagta
    37621 gagagtggca gtttaaagta tagaatccca gtaaaaggta atgatgaatt atcatcactt
    37681 gcatctagta ttaatgagat gagtcataca ttggagaatc aacttataaa agaaagggag
    37741 atgaagaata aaaataaaga tatagtaact tcaatatcac atgatgttag aactccatta
    37801 acgtcagtaa tttgttattt agatttgatt aaagaccata aatacaatag tcctgagcaa
    37861 ttggaacaat acataaaaaa tgtaaggtct aaagcatatc aaataaaaga tttaacagat
    37921 gaccttttta cgcattttgt taattctgga gaggaagttt caaaagggta tgaaaaaata
    37981 aatgctaatg aatttatttt tcaattactt ttagatagtt catatacatt ggaggagaaa
    38041 ggatttcata taacaataga aaatgaactt tataaccaat atcatattta tgtagatgtt
    38101 acacaattta gaaggatatt tgataattta tgttctaata taataaaata tgctaggaaa
    38161 gataagccta tagtattttt aattaaagta gaggattcta aattactaat tatacaaaaa
    38221 aatagagtaa gtaatttttc taagaatgta gaaagttttg gaattggtat aaagaattgt
    38281 gagaaaatag ttgcatatca taatggaagc ataaatgtaa attcagatga taaagaattt
    38341 gctattgaaa tatctattcc agcaaaagaa tcataaaaat ttaaattata tattgtgtag
    38401 ttgctattat atattgtgta attgcttatg taaaattcta tgaagcagga agcatgtgac
    38461 tttagttatg ggatgttcaa tattgtgtac ctctaggcaa gtaacaagtt tacttacaag
    38521 accatataat attattatct taaaaaaagg ggacataata atatggtttt gacaggagca
    38581 cattatattt acattctatt tatggtaatt atattaatta ctatgattat gaaaaaggat
    38641 acaatagtac catgtatatt aggtgttttt tttatgggat tgttctttga aaaaaacatt
    38701 tttggttcta taacagcagt gtttaactct tttattatat ctttaaatga gttgggtcca
    38761 attatcttga ttatagctat tatggttgca ttatctaaag ccctagaagc caataatgct
    38821 atacaatata tggtgagacc attttcgaga gttataaaaa attctaatac agcatttttt
    38881 gtaactggat ttgttatgtt agttctttct tggtttttct ggccaactcc agctgttgcc
    38941 ttagttggag ctgtattttt acctgttgct atgagagctg ggctaccagc aattggtgtt
    39001 gcagtagcat taaatttatt tggtcatggt cttgccttat ctacagactt tgttattcaa
    39061 ggagctccaa gtataacagc tggtgcagca ggtgttgcag tatcatatgt tataaatgat
    39121 ggtatgattt tgttttgggt gatgggaata gtaaccatat ctatggcatt ttatacatta
    39181 aaaagagata ttaacaaagg aatgtttaga gaagaactaa aaggttttga aagtgaagaa
    39241 gtagaagagt ttaatggaaa atctaaaata gcaactattt tagtagcttt gggatttctt
    39301 gctgatataa ttgctatgta tgtatttgac ctaaaaggag gagatgcatc agctcttttg
    39361 ggtggaacag cagtattttt aataataatt ataaatgtta taaattttgg caaagatagt
    39421 cttgaaaatg tatgtgagaa tataattgat ggttttgtgt ttgggataaa aatatttgga
    39481 gcaattatac caatagcagc atttttctat atgggtgaag ttgcaccctt gacaggtgta
    39541 tttggaaaag tattagcacc aggttcacaa ggtttattat cagatatagg catagctctt
    39601 tctcaaacaa taccacttaa taaatttgcc gtatcaggta tagaaactgt agtgggagca
    39661 ataactgggc ttgatggttc tggattttct gggatttcat tagttggttc acttgcctct
    39721 gtatttggaa ctgctataaa tgcaagtgtt ggtgcattag ctgcattagg acaaatcagt
    39781 ggtatatggg taggtggagg ctgtctagtt ccttggggtc ttatttcagc agcggcaatt
    39841 tgtggagtta gtccaataga attggcaaag cgaaactttg tcccagttat aaccggattg
    39901 gttgtaacaa ccatagtagc aatgtttatt atttaataaa tcgaataaat ctttaattgt
    39961 ataaattaaa aatagatgtg tagctataat ttattataat tgttttacta attctaagcc
    40021 atacatctat ttttattgat atttttatta gtattcttta ataaaataat atttttcatt
    40081 atactataca taacatctaa atataacatc taaatataac atctaaaata ctgttatcta
    40141 tatttaatat cttgccaaat tttttgatgt tttattgccc tgtcccacca acatatataa
    40201 gctatcacaa gtttttacct atactatcac caaacaaaaa ttctaatttt aatttattat
    40261 tttataacct atattatatt aactatatta gtttgtagac tttagatgta attataaata
    40321 ttaatactcc aagagttcaa attcatatcc tagttttctt gcatttgata taaaatcatt
    40381 taaaatttct gtactagttt tggaaactgc atgtagtaac aagatagaac catcatgtag
    40441 attatccatt attttttgcg ttgcttcttc atgtgatggt tgtttatcag tatcccagtc
    40501 tctgtatgca aaactccaaa atacagtctt gtatcctaaa tttttagtca ttgccaagct
    40561 tttctctgag tattttccca taggaggtct aaaaaattta accatatctt tcccagttac
    40621 atctttatat aacttttcaa catcatataa ttcgtcatta aagtttttta gattgcttgt
    40681 ttttgttggc atagatggat gggttttact atgatttcca actatatgtc cctcagaaac
    40741 cattcttttt actaaatctt tattttcttt tatatatgga gctgtaacaa aaaaaactgc
    40801 tttaacctgg ttttgtttta gagtgtcgag tatttttgta gtgtatccat tttcataacc
    40861 ttcatcaaat gtaagatata gtgttttttg accatctttt gtaggtccat tgtatagggc
    40921 atcatagtca gaaaatttaa attcaatgtc cgtatttact tctggtgttt tatgcttatt
    40981 gtttggtata taaaaccaat caagagtagt gttatctaaa gaagatacat ctaatttatt
    41041 attatcatta ttagtagtgg cttttgatgg tttatctgtt ccgtctgaaa ctattacatt
    41101 ggaagtatct ttaccactat ctattttttc cttatcttct tgtaactgtg tttccttatt
    41161 ttgattttcg ttttgattgt tttgtgaatt tgaacatcca acaataaaaa aaatagaaat
    41221 agaaagtagt gttaaataat gcaatacttt tctaaacatt ttgaagctcc tttctaaatt
    41281 aataataatt gcatataata ataattatat gattaattac aattgcatat agtattatta
    41341 taattggaaa ttactattaa taatagttat atctttaaaa ataaagtcac aatagtatga
    41401 caaagggtga ataatcaata aatttaaaaa aaaatgaaaa aaatagttga ccatattatg
    41461 agaagatgtt attataataa atgtgctcaa caagaagtaa caaaataaca agcacaaaga
    41521 agttaattga at
//
LOCUS       DAEORZ010000019        40871 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEORZ010000019 DAEORZ010000000
VERSION     DAEORZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40871
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.019"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            165..1160
                     /gene="ilvC"
                     /locus_tag="KRH39_000895"
     CDS             165..1160
                     /gene="ilvC"
                     /locus_tag="KRH39_000895"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBG8510817.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VMIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            1213..2901
                     /gene="ilvB"
                     /locus_tag="KRH39_000896"
     CDS             1213..2901
                     /gene="ilvB"
                     /locus_tag="KRH39_000896"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBG8510818.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEVLEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWSAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            3112..3789
                     /locus_tag="KRH39_000897"
     CDS             3112..3789
                     /locus_tag="KRH39_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG8510819.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNKDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            3963..5999
                     /locus_tag="KRH39_000898"
     CDS             3963..5999
                     /locus_tag="KRH39_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBG8510820.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIKALKDDFDVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKDGGFYSAQDADSEGEEGKFYI
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNENCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDSRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIISFLNNYRLKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(6193..6759)
                     /locus_tag="KRH39_000899"
     CDS             complement(6193..6759)
                     /locus_tag="KRH39_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8510821.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVVPSPHDKRAINVRV
                     TDLGKKVMVTCNRTGINFMADVFHEFTKDELETLWNLLRKMYRFNGEEQDGFEEDVNF
                     MEYEEIDKIKSEALEEFAKRRNRVNKND"
     gene            7047..7982
                     /locus_tag="KRH39_000900"
     CDS             7047..7982
                     /locus_tag="KRH39_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HBG8510822.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINKEEVFEDIICWLNNKIEIL"
     gene            8446..9660
                     /locus_tag="KRH39_000901"
     CDS             8446..9660
                     /locus_tag="KRH39_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8510823.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEVDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHENLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKILKNKE"
     gene            complement(10080..10601)
                     /locus_tag="KRH39_000902"
     CDS             complement(10080..10601)
                     /locus_tag="KRH39_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8510824.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSSLQEEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            10957..11754
                     /locus_tag="KRH39_000903"
     CDS             10957..11754
                     /locus_tag="KRH39_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG8510825.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            11864..12454
                     /locus_tag="KRH39_000904"
     CDS             11864..12454
                     /locus_tag="KRH39_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBG8510826.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGARNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNIRADWSCFTDFNIVSQNLESYSYWIKKFCMTFNTDFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            12519..14216
                     /locus_tag="KRH39_000905"
     CDS             12519..14216
                     /locus_tag="KRH39_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG8510827.1"
                     /translation="MDYIKTYEEWIKGSYFDEDTKLELEKIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKNQII
                     RKDLVKNVGKSFKIVYTPLCGTGNIPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKSNNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            14430..15269
                     /locus_tag="KRH39_000906"
     CDS             14430..15269
                     /locus_tag="KRH39_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBG8510828.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFLKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLIGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            15469..15606
                     /locus_tag="KRH39_000907"
     CDS             15469..15606
                     /locus_tag="KRH39_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510829.1"
                     /translation="MVEASRNILNKNYSPNELMSVFSNNAVFSHVFLYAPVFLITNIL
                     E"
     gene            15787..17643
                     /locus_tag="KRH39_000908"
     CDS             15787..17643
                     /locus_tag="KRH39_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG8510830.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVIENKKVVTFELNVSINDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKEDGKINIFL
                     YVKDNIFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    17725..17965
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    18031..18266
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            18351..19568
                     /locus_tag="KRH39_000909"
     CDS             18351..19568
                     /locus_tag="KRH39_000909"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG8510831.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLER
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            19685..20167
                     /gene="cdeM"
                     /locus_tag="KRH39_000910"
     CDS             19685..20167
                     /gene="cdeM"
                     /locus_tag="KRH39_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBG8510832.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYERAAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYQGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            20432..21697
                     /gene="hisD"
                     /locus_tag="KRH39_000911"
     CDS             20432..21697
                     /gene="hisD"
                     /locus_tag="KRH39_000911"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBG8510833.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(21900..22652)
                     /locus_tag="KRH39_000912"
     CDS             complement(21900..22652)
                     /locus_tag="KRH39_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8510834.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQTRKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMSTFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFNY"
     gene            complement(22770..23039)
                     /locus_tag="KRH39_000913"
     CDS             complement(22770..23039)
                     /locus_tag="KRH39_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510835.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            23514..24608
                     /locus_tag="KRH39_000914"
     CDS             23514..24608
                     /locus_tag="KRH39_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG8510836.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISNITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            24610..25559
                     /locus_tag="KRH39_000915"
                     /pseudo
     CDS             24610..25559
                     /locus_tag="KRH39_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
     gene            25552..26736
                     /locus_tag="KRH39_000916"
     CDS             25552..26736
                     /locus_tag="KRH39_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510837.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISNYFESKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            26749..28245
                     /locus_tag="KRH39_000917"
     CDS             26749..28245
                     /locus_tag="KRH39_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG8510838.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            28268..29218
                     /locus_tag="KRH39_000918"
     CDS             28268..29218
                     /locus_tag="KRH39_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8510839.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            29239..30201
                     /locus_tag="KRH39_000919"
     CDS             29239..30201
                     /locus_tag="KRH39_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG8510840.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            30573..31205
                     /locus_tag="KRH39_000920"
     CDS             30573..31205
                     /locus_tag="KRH39_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG8510841.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            31616..31708
                     /gene="kdpF"
                     /locus_tag="KRH39_000921"
     CDS             31616..31708
                     /gene="kdpF"
                     /locus_tag="KRH39_000921"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBG8510842.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            31749..33431
                     /gene="kdpA"
                     /locus_tag="KRH39_000922"
     CDS             31749..33431
                     /gene="kdpA"
                     /locus_tag="KRH39_000922"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBG8510843.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            33456..35513
                     /gene="kdpB"
                     /locus_tag="KRH39_000923"
     CDS             33456..35513
                     /gene="kdpB"
                     /locus_tag="KRH39_000923"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBG8510844.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SALVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            35532..36158
                     /locus_tag="KRH39_000924"
     CDS             35532..36158
                     /locus_tag="KRH39_000924"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBG8510845.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEQFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            36395..37303
                     /gene="cysK"
                     /locus_tag="KRH39_000925"
     CDS             36395..37303
                     /gene="cysK"
                     /locus_tag="KRH39_000925"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBG8510846.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            37330..37920
                     /gene="cysE"
                     /locus_tag="KRH39_000926"
     CDS             37330..37920
                     /gene="cysE"
                     /locus_tag="KRH39_000926"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBG8510847.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            38080..38268
                     /locus_tag="KRH39_000927"
     CDS             38080..38268
                     /locus_tag="KRH39_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG8510848.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(38322..39191)
                     /locus_tag="KRH39_000928"
     CDS             complement(38322..39191)
                     /locus_tag="KRH39_000928"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBG8510849.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(39388..40632)
                     /locus_tag="KRH39_000929"
     CDS             complement(39388..40632)
                     /locus_tag="KRH39_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510850.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT        15876 a         4268 c         7246 g        13481 t
ORIGIN      
        1 ttagtttgtt gtagctaata aaaactgaaa attctaaaat tataattgta agattatata
       61 aatttagaat ttaaatctgt tgaataatat taaaacaatg ataatgagta tgaatttcta
      121 ataaaaataa atatcataaa aataagaaaa agtattggga ggaaatgaaa atggcaagaa
      181 tgtattatga aaaagatgtg gatttagagg ttttaaaaaa taaaaaggtt gcagttttag
      241 gttatggaag tcaaggacat gcacatgcac aaaatcttag agataacggt gtacatgtaa
      301 tgataggtct ttatgacgga agtaaatcag cacaaaaagc aaaagaagat ggatttgaag
      361 ttaagagtgt agcagaagct acaaaagaaa gtgatttgac aatgatgtta atgcctgatg
      421 aaaaacaaaa gaaagtttat gaagagagtg taaaggataa cttaaaagaa ggtcagacat
      481 tagcttttgc tcatggattc aatatacatt ataatcaagt acaaccacca gaatttgttg
      541 atgttgtaat ggtagctcct aaaggacctg gtcacttagt tagaaatgta tttacaaagg
      601 gaagtggagt tcctgcacta tttgcagtat atcaagacta cactaaaaaa gctacagaga
      661 cagttttagc atatgcaaaa gggataggag caacaagagc aggtgtttta gaaactactt
      721 ttaaagaaga aacagaaaca gatttatttg gagaacaatc agtactttgt ggtggaataa
      781 gtgaacttat aaaattggga tataaaacgc ttgtagatgc aggttatcaa aaagaagtag
      841 catattttga atgtttacat gaaatgaaac taatagtcga ccttatatat gaaggtggat
      901 ttgaaagaat gagatatagt ataagtgaca cagcagaata tggagattat gtatctggta
      961 aaagggtaat aactgatgct gcaaaacaag gtatgcaaaa tgttttagag gatatacaaa
     1021 atggaaaatt tgcaaaagcg tggattaaag aaaatgaaga aggacgtgaa aacttcctaa
     1081 aaactagaga agaagaatat aatacagaaa ttgccgaagt tggtagaaat ctaagaagta
     1141 tgatgtcatt cttaaaataa atatatatta aatggattag tttgagggaa agagtggtgt
     1201 atagtagtgc gtatgaatgg agcaaaggta attttagaat gtttaaagaa agagggaatt
     1261 gacacaatat ttggatatcc tggtggagct gtgatacctc tttatgatgc tctatacgat
     1321 tattcagatg attttaaaca tataaggaca tcccatgaac aaggtttagt tcatgctgca
     1381 gatgggtacg caagaagtac taatacagta ggagtttgtt ttacaacatc tggtccagga
     1441 gcaacaaatg ccataactgg aatagcaaca gcttttatgg attcttcacc tatggttgta
     1501 atttcagggc aggtgccaac tagtttatta ggaaaagatt cttttcaaga aattgacata
     1561 actggagcaa cattatctat gacaaaacat aattatttag ttagaaacac taaagaatta
     1621 gttcctacaa taaaagaagc ttttagagtt gcaaattcag gtagaaaagg acctgtactt
     1681 gtagatgttc caaaagattt atttttagca gaaatggatt ttagtggtga ggactatgat
     1741 ttatgtcaaa tagatgatta tatggattat aagagtgatt ttgatctaga tgatgaaact
     1801 aatattaagc ttttaaatga agcaatagat atcataaaag aatctaaaaa gcctgtaata
     1861 tatgcaggtg gaggtgttaa atcttcagac agtgaggaag ttcttgaaaa atttgcaact
     1921 aagatagata cacccgtact aaatacactt atggggcttg gaaatataga tagaaagaat
     1981 gaactatctc ttggaatggt tggaatgcat ggaagtagag aaagtaattt agcactttct
     2041 aattcagatt tagtgatagc gataggtgcg agatttagtg atagagtgat aagtaaaagt
     2101 tctgaatttg ctaaaaatgc aaaaataata cacatagaca tagacccatc tgaaataagt
     2161 aaaaatatag aatctaatgt atctttggtt ggagatgtaa agctagtcct tagtttatta
     2221 attgaaaggg ttgaaagtaa gaataatagc aattggaaag aagagataaa gacatttaga
     2281 aagagtgagg gtgtacaaac atatgaattt catccacaaa atatacttaa aaaaataaat
     2341 gaaaaatatg aaacattaaa gaaacccact gtggtagtaa ctgatgtcgg tcaacatcaa
     2401 atgtggtctg ctaaatactg gaattttaaa ggtaataaaa gttttataac atcagcaggg
     2461 ttaggaacta tgggatttgg tttaggtgca gctattggaa ctaaagttgg taatgtagat
     2521 aaaaatgtag ttttagtcac tggtgatgga agttttagaa tgaactgtaa tgaactagct
     2581 acagttgcaa attataatgt acctatgctt attttactac tcaataatag aacattagga
     2641 atggtaaggc aatggcaaaa gttattttcc aatcaaagat actctcaaac tgacattaat
     2701 gaaaatgtgg attatgttaa actcgtaaat gcatataata ttgatggata taaggtatcc
     2761 agtatggaag aattaggaaa ggcgctagat atgatagatt ttaataaacc tgtatttttg
     2821 caatgtgata tagataagga ttatgatgtt tatcctattg tagcacctaa tgatgcattg
     2881 gaaaatttga tatgtaacta aaacttatac tgaatattga taaaattttt tattaaattt
     2941 ttattggtat tcagtttttg ttgtttagag atagattggc attttttatt ttttcaaata
     3001 aatcaatgac tatataatta agaacttatt aaattttaat tatacaattt ggaatagtat
     3061 tacaacttaa gtaaataata attttgaata ataatttacg gaggtattca tatgtttaaa
     3121 cacgataaag cattattaaa agaagtaaaa gtagaaagac caaatcccca atatgctgtt
     3181 ttaatgcaag aacagttggg tggtgcaaat ggagagttaa aagcagctat gcaatacttg
     3241 tcccaaagtt ttagaataaa agaccctcaa ataaaagatt tatttcttga tatagcagca
     3301 gaagagctta gccatatgga aatggtagca caaactataa atttattaaa tggacatgat
     3361 gttgattata atgcagttaa tacaggcgaa atagaaacac atgtattaac tggtctatca
     3421 ccagttttaa taaattcatc aggagctcct tggactgcta attacgttac agttacaggg
     3481 gatttagtag ctgacttact ttcaaacata gcttctgaac aaagagctaa ggtagtttat
     3541 gagtacttgt atagacaaat agatgataaa tatgtaaaag aaacaataga tttcttactt
     3601 aatagagaag aagcacataa tgctctattt agagatgctt taaataaagt taaagataca
     3661 ggttcaaata aagattttgg agttacagaa gattctaaat tatatttcga tttgtctact
     3721 ccagggccaa atcacgatac taagatagat attaatcctc cttcttttga aaaaccttta
     3781 aaaaaataat ttgatatttt ttatgttgtt ttgttttagt agttataaaa attatttatt
     3841 agaagaatta attatatgaa aaattaaata tgtatagtta aaaatgggta tacacttatt
     3901 ataagtgtat atctatttgt ttattgcaaa acatataata aaataacaaa tgaggtggta
     3961 ttttggacag taatagaaaa cctaataatc taataaatga aaaatctcca tatcttttac
     4021 agcatgctta taatcctata aactggtata gttggaatga tgaggctttt aaaaaagcaa
     4081 aagaagagga taaaccaata tttttaagtg taggatattc aacatgtcat tggtgccatg
     4141 ttatggaaaa agaatctttt gaagatgaag aagttgctga aataatgaat agaaactttg
     4201 tggcaataaa agtagataaa gaggaaagac ctgatgtaga tagcgtgtat atgacagttt
     4261 gccaagctat gacaggtagt ggtggatggc caatgaccat aataatgaca ccagataaaa
     4321 aacctttttt tgcgggaaca tatttcccaa aatattcaag gtataataga cctggagtta
     4381 tagatttatt agagaatgta tctgaaaaat ggaatacaag cagggatata ttaataaaat
     4441 ctggagatga aataataaaa gctcttaagg atgattttga tgtaaaaaat actgagggtg
     4501 atttatcaaa agagatgttg agttcatcag ttagagtatt taaggctatt tatgacgaaa
     4561 agtatggtgg ttttggtaat gctcctaaat ttccaagtcc tcagaatctt atgtttttga
     4621 tgaaatatta tagtatagaa aaagataaag atgtgttaaa aatggtggaa aaaactttag
     4681 atggaatgta tagaggaggc ttgtttgatc atattggatt tgggttttca agatactcta
     4741 ctgataaaaa gtggttggct cctcattttg aaaaaatgct atatgataat gctatgctta
     4801 ctatagcttt tttagatgcg tataaaataa caaagaaaga gttatataaa gaaattgcta
     4861 taaaaactat agattatgta gttagagaga tgaaggataa agacggtggg ttttattcag
     4921 cacaggatgc tgatagtgaa ggtgaagaag gtaaatttta tatatttaat cctcttgaga
     4981 taattgaagt tttgggtgaa gaagatggaa ctttctttaa taattatttt gatataacta
     5041 gtagtggaaa ttttgaaggc aaaagtattc ctaatcttat aaaaaataaa gaatatgaga
     5101 gacataatga aaagatagct gatcttagta aaaaggtatt tgaatataga aaagaaagaa
     5161 catctttgca taaagatgac aagatactta catcatggaa tgcattgatg atagttgctt
     5221 taacaaaggc atatagtact ttaaaaaatg acatatattt agagtattca aataagtgtc
     5281 ttaactttat aaataataat cttgtaaatg agtcaggtag attattagct agatatagag
     5341 atggaagttc cgactattta gcatatttag atgattatgc ttttttaatc tgggcgtata
     5401 tagagcttta tgagtcaaca tttaatatga aatatttaga aaaggcttta aatttgaatg
     5461 aaaactgtat caaccttttt tgggattatg aaaaaagtgg attttatata tatggtaaag
     5521 atagtgaaaa tttaatagca agaccaaaag atttgtatga tggagctatt ccatcaggaa
     5581 attctgttca attatacaat cttataagac ttgcgaaaat tactggagat agtagacttg
     5641 aagaaatgtc atataaacaa ttaaaactat atgtggataa tgtaaagagt tctccaactg
     5701 gctacagttt ttatatgctt tcattgatgt ttgaacttta ttctactaag gaaattatat
     5761 gtatttttaa ggaagattct gatttaattg catttaaaga attgataagt gaaaatttta
     5821 taccaaatgc aactttttta gcaaagaaat acaatgaaga aaacactatt ataagttttt
     5881 taaataacta tagattgaag gatgataaga ctagttatta tgtgtgtcaa agtaatagtt
     5941 gtagccaacc aattaatgat ttacaaaaat taaaagatat gattttagga gtagaataac
     6001 cataatagtg aaaaaggaga tgctttaaaa taaaagtcta atcatctcct tttttgtata
     6061 aaaaaacaga aaaagagttc ctaagaacac ctaatctatg taatgattgt tttgttaaaa
     6121 aataaaaatg acattatagt tatataaaat attgtactat aaaaaagtgg tattattata
     6181 gatgctatag tattaatcgt ttttatttac cctatttctt ctttttgcaa attcttcaag
     6241 tgcttcagat ttaattttat ctatttcttc atattccata aaattaacat cttcttcaaa
     6301 tccatcttgt tcttcaccat taaaacgata catttttctt agtaaattcc ataaggtctc
     6361 caattcatct ttggtaaatt cgtgaaatac atctgccata aagtttattc cagtcctatt
     6421 acatgttacc ataacctttt ttcctaagtc tgtaactcta acattaattg ctctcttatc
     6481 atgaggactt ggaactacat caacatatcc atttttttca agattggcaa ctagtctatt
     6541 tatattttgt ttacttgtac ccatttttct tgcaatatta tttaatgttg tttcttcctc
     6601 tggtaaatga agaattgata aaatagtcat atactgtctt gaagtaagta taccaaagta
     6661 tttatctcct tctatttgta ttttgtttgt aagggtaaaa agagttgcat atgtttgatt
     6721 cattaaaaag agctctttta attcatttga ataatccatg aaatatatct cctttatttt
     6781 tagatgttgt tttgtcgaaa acagaatatc atgaattaat tctaatgtca atatggtgtc
     6841 aaaatatatt aaagtattac ttttttatgt aactattaat taattaaatt attgtataat
     6901 tttatgtaaa aacttctatg taccaatgat aatttctatg aggaattaat aattcttaaa
     6961 ttttttagaa agattttgac ttgaaaatac ttgtaatata atatataaat taatatactg
     7021 aataaaatca atctggaggg ataattatga agtgtacaaa ctttactttc aaaggagagg
     7081 aaggtttaga tatatataca tataaatggg aagatgaaaa tataaaaaaa ccaaaggcag
     7141 ttatccaaat tgcacatgga atggctgaaa ctgcacaaag gtatgagact tttgccaagg
     7201 tacttactaa aaatggttat atagtatata tcaatgacca tagaggtcat ggaaaaactg
     7261 caaagataat agaaaatgtt ggacatttag ctgaaaaaga aggatttaga tgtcttgtag
     7321 aggacatgta tactttaaca aatattataa agaaagaaaa tgaagatttg ccaatttatc
     7381 tatttggaca cagcatgggt tcatttgcaa gtcaaagata tataatggat tacagtaata
     7441 atttatctgg tcttatacta tgtggttcaa atggaaagca aggaatcatt ctaaatcttg
     7501 ctcatctgat aatcaatcgt gagattaaaa agtatggaag acgttctaaa agtaataaaa
     7561 taaataattt gatatttggt ggcgaaataa taagaagaaa tgaaaagact aagtttgact
     7621 ggttaagtag agataaagaa caagttgaaa aatatataaa tgacccattt tgtggagtag
     7681 tgtgtagttg tggctttttt tatgatttag ttcaagggtt aaaagaaatt gaggataaag
     7741 aaaacttaaa aaaagtgcca cttgatatac ctatttacat aatatctgga gataaagacc
     7801 ccataggaaa aaatggtaaa ggagtcttaa gattaaggga tagatatata aaattaggag
     7861 taaaggatgt aacttgcaaa ctttacaaag atggaaggca tgaattgttg aatgaaataa
     7921 ataaggaaga agtttttgag gacataattt gttggctaaa taataaaata gaaatattgt
     7981 aataaaaaaa tataatgata tatttgcttt aaatttattt atatataata tttgaaaagt
     8041 ggattatgta tatgcaaagt agaaaagaga gagcctagaa gtgatttttt tactataatg
     8101 ccatttattg aatagatgga tattattaca aacaaattat taacactcag tattgttatc
     8161 attgtatatg aaatttattg aaagtctaaa tatagtaagt tgtagtaaag aatagtaagt
     8221 tgtagtaaag agaaaatcta aatatttaaa tggctattta atgtgaatct attaattagg
     8281 ttaaacttaa ttaatagatt ttatatttta ggtcaatgaa agaagatttt agattaaaat
     8341 ataaccaatg gttaaattac agacattttg ttaactttcc ctcgatttac tagtaaatat
     8401 tttttttata tacttaaatt acaacagatg tactaggagg tatatatgca aattaaaata
     8461 ggtaaagtca tacagcgtct tagaaaagaa cgaaatttaa ctcaagaaca attagcaaag
     8521 tttataggtg tgtcaacacc agcagtatct aagtgggaga gtggaaattc ttacccagat
     8581 atagaattat tgcctttatt agcagacttt tttaatgtgt ctattgataa gcttttaaat
     8641 tacaagattg atttaagtga agaagaagta atgaaaattt ataaagaatt agaatcaggc
     8701 tttgctagaa ttgaagttga tttatcaaca gaagaaccaa aagaagaatt tagacaagac
     8761 ttagaatctg taaaaaagct ttccaatatg tatatagaga agtatcctaa aagctattta
     8821 ttgaagctaa ggatatgttc gctgtatcag atgtattcat ataaatttgg taagagtgaa
     8881 cttaatgaca gagtaaaaga aacaacgaat attcttgaag atatagtgag aaatacagat
     8941 gatatccaaa taaaagaaac agctcttatt atattatcaa acgcatattg tatgttggag
     9001 gattatgaaa aggcagagtt gtatttaaat atgatacata agtcaatagg ggatacaagt
     9061 gttaatttag ctatgatata tttaaagcaa aatagacttg aagaagcaga aatattgctt
     9121 caaaataaat tgtttagtaa tgtatttaat ataagcatgg attgtaaggg aataattaat
     9181 gtatacaaaa atcaatacaa ggaattaaag aaaaaattgg agaatagaaa ttttaataaa
     9241 aatgcaattg agagagaaat ggagtatata aaaaataagt tgctggggta tgcaaatctt
     9301 tctttagaaa taaaaaaaat gctcagtgaa gataaaggtg cattttttag tatgtatatg
     9361 gattatatgg aattatctct aattttttta ttttttaaca tgaaagaaga agcaaaaaaa
     9421 gccttatata gtctaaaaga aatattagaa aaatatccta tacatgaaaa tttggatgta
     9481 agtcaaatga gattttttga taaggtagag tctaaaaatt tatatacttt taatatatat
     9541 acaaacttgt taattgtact taatgatgat agttataatg agcttagaga agaaccaatt
     9601 tttaaggatg taattgaaaa gattttagat atggaaaaaa tattgaaaaa taaagaataa
     9661 gtaaaaattt aatataggat ttaaagatta atcaaattaa aagaaagtgt ttcaaaataa
     9721 aaatttgaat cacctttttt gtcggtctta aacagttatt tagaaaaata gaggtttcaa
     9781 aatagaaatc tgaattatct gtattattac acaaaaatag aaacctaaat ttataaaaat
     9841 agaataaagt ttgttatata aaatgtaatt ttgtctataa aatctcacct gttttcttaa
     9901 ctttaagcgt gaaaaagagg atgtatcatg aattttttag ttcatgatgc atccttcttt
     9961 gtatgaaatc caatgtatct ttattattta aacaatggaa aagagtctta tttatatttt
    10021 gagacatctc ttttatttaa ttatgaagtt ttatacagtt caataaatag gaattatatt
    10081 tagaaactca tgacattatt tcttagatag tgattaaatt tatcatattc cattttagat
    10141 aacttttctg ggtaagacat tacaatcagg ttatgcccat taaatttttc atggtatggt
    10201 ggatgaatgt gttcataatt atttgtctta tattttactc tttcatagaa cgccttgcga
    10261 taaattgaaa tctcatcaat tggaaggtca atttctaaaa taatagtttt tcctttctta
    10321 tttaaaagct ctaacgctct ttgtccatat ctattattgc gcatttctgg aaaaatgcag
    10381 aaatgttcta catagataaa gtcatctgtt tcccaacata aaattatgcc gacaaactgg
    10441 tttttatcat aaattaaatt gaattgatat tctttatttt tcaaaatttc ttcttgcaaa
    10501 gaagattttc tttgttcatg aaatgggaaa ctaattttat ataattccat agccttgtaa
    10561 tataatttat tttcactggt tcttaaccgt tcaaatttca ctttactcct cctaatcact
    10621 aaaattccaa tttatttatg agttcatttt accatagtca gtatttgcat acaatgtgtt
    10681 ttaagttgag tgtcatttct ttcttttatt tatgtttata tctcatagac taatgtataa
    10741 ttgcttaaga cataacttaa aactattata ttaagctttg ataatctaaa aacagtatat
    10801 ttatggactt ttctaggtct aaaattaatc ttaataattt tagaagttac aaaaaagtta
    10861 cataaatttg gaaagctata atatacatat ataatataag gttaaaactg taatgaataa
    10921 tattaattta aaatttgaac atggaaggag attattatgg agaaaaggga aaagaggata
    10981 ataatatcat ctttattaag tgtatcaatt ttaatgggtt tggtaagtat atattctata
    11041 ttaaataaag aagacataag tttaacagtt aaaggtcaag agcaaaaagt atcttccttt
    11101 aagaaaacag ttgaggaact tttggatgaa cagggtgtaa agtataattc tgaagataag
    11161 attaatccaa gtttagatac agaactaaaa gatgatatga aaataaaagt tgttaaggta
    11221 actaaaagta aaaaagaaga gattgaaaaa attccatttg atacaaagca tgtaaatgat
    11281 agtaatttgt taaaaggaaa atctaaagtt tatcaagaag gtcaagaggg agaaaaaaaa
    11341 ctagtctata atttaactta ccatgatgga aagttagtca aaaaagtctt atcaaaagaa
    11401 gtaatatcta aggagccaac tacaaaaatt ataaaatatg gaactaaaga aaaagtatta
    11461 atagcatcaa gaggagcaaa tataagagga ggcaaacata tgaaagtggt tgccactgca
    11521 tatgcaggag atacaataac atctactggt acaactccaa gatggggtgt tattgcagtt
    11581 gacccacgtg taataccata tggaacaaaa gtatatatac ctaagttggg tatgactttt
    11641 gtagcagaag attgtggagg tgcaattaaa ggtaatagaa tagatatttt tatgaacagt
    11701 gaaggaaaag cctcaaattg gggaaggaaa agtatagata tatatctaca ttaaattagt
    11761 gaatagaata actttacttg aaggacaaaa aagtataaaa tatgaattaa ctaaactgtt
    11821 agattattct taaaacataa aaatatatct aaggggggat aagatgaaaa tagtctgctt
    11881 aggagacagt ttaacatatg gatttggagt atcacgaagt aattcatgga caaacattgt
    11941 aaataaagag actcgattag aaatagtaaa taagggtata aatggtgata caacaagtgg
    12001 tatgttagct aggtttaatg aagatgttgt aaaaaactct cctgatatag tttttataat
    12061 gggtggcacc aatgatttta ttgctggtgc gagaaatgaa attattaatt ctaatataat
    12121 ggctatggtg catcagtcat attcaaaaaa tattatacct ataataggta taccattaaa
    12181 accagatatt ccaaatatta gagcagattg gagttgtttt actgatttta acatagtatc
    12241 tcaaaactta gaatcatata gttattggat aaaaaaattt tgtatgacat ttaatactga
    12301 tttcgtagat ttttattcag aatataataa aaatatggaa ctagaaggat ataaaaaatt
    12361 atattttgat gggttacatc caaccaagga aggtcataga ataatggcag acatttttat
    12421 taattcaatt aataagtata tagaagagtt atagattaca ttaaaatgct tgtttatgtg
    12481 tcagttgcaa taatacatac agtagagagg ggtttattat ggattatatt aaaacttatg
    12541 aagagtggat aaagggttca tattttgatg aagatacaaa gctagagttg gagaagataa
    12601 aaaataatga aaaagagata gaggatagat tttataagga tttagaattt gggactgcgg
    12661 gtcttagagg aataattgaa gcaggaacga atagaataaa taagtacaca gttagaagag
    12721 ctacttttgg attggcaaac tatatattag aaaacacaac aaaagaagaa acaagtagag
    12781 gagtagtaat agctcatgat aatagacaca agtctagaca attttgtata gaatctgcaa
    12841 atactttggc agcatgtggt ataaaagcat atatatttga tagtttgaga actactccag
    12901 aattatcttt tgctgttaga agcttaaatg caatcgctgg tatagtcata acagcaagcc
    12961 ataatcctcc agaatataat ggatataagg tatactggga agatggagca caagttatgc
    13021 cagagattgc aaatgcaata acagagaaag taaatagtat acatgattat agtacaatac
    13081 caaccttaac agaggccaat aaaaatttgg tggttttgtt ggatgaatca caagatacta
    13141 aatttataga agcagtaaaa aaccaaatta taagaaaaga tttagttaaa aatgtaggta
    13201 aaagctttaa gatagtctat acacctcttt gtggaacagg gaatattcca ataagaagag
    13261 cattgaaaga agttggtttt gaaaatataa ttgtagttcc agaagaagaa aatccagacc
    13321 caaattttgc agggcttgat tatccaaatc cagaagagaa aaaagcttta aatagaggta
    13381 tattactcgc aaaagaaaaa ggagcagacc ttgtaatagc aactgaccca gattgtgata
    13441 gagtaggtgt agcagtaaaa actactacag gtgagtatgc tttacttaca ggaaatcaaa
    13501 ttggtggtat gctgacacat tatataatag aaggattaaa agaaaataat aagttaaaag
    13561 aaaatcctac aataataaaa accatagtta cgtcagaatt tggagcagat atagctaaat
    13621 caaataatgt tgatgtatta aatgttctta caggctttaa atttattggt gaaaaaataa
    13681 aattatttga acaaaataaa aatagaagtt atgtatttgg atatgaagaa agttatggtt
    13741 acttagttgg gactcatgct agagataaag atggtgtagt ttcttcattg ttaatatctg
    13801 aaatggcagc attttactat tcaaaaggta tgagtttata tgatggttta atagaacttt
    13861 ataaaaagta tggatttttc aaagaacaga ctatatcttt gactttaaaa ggtatagaag
    13921 gtgtagaaaa gataaaagaa ataatatctt actttagaga aaatcaaatt gattatataa
    13981 atagtattaa agtagttgat aaaaaagatt ataaaaatgg tatagataat cttcctaaat
    14041 ctgatgtttt aaaatacttt ttggaagatg aatcttgggt agctataaga ccatctggaa
    14101 cagaaccaaa acttaaattt tacatagctg taaaaggagc tagtgatatt gaagcagata
    14161 aaaagattca agggttgaaa aaatatatag atgacatggt cgaaaaatta aaataaaata
    14221 ttattatttt ataagagaat tttgtaataa tatttattta gctgaaaatt ataatttact
    14281 ttcattgagt agaatatgga aatgttagta gaattattat tttcagcttt tatttgtgtt
    14341 atttttactt aaataattat attatagaat gaatctgagt ataattaata ttgtataatg
    14401 aaattaattt gatagaaaag gggatattta tgtttggtat agataagaaa ttgattgaat
    14461 atataattgc aaaatccaat tacagtgaat taagttcaaa gtcagcagaa gtatctttct
    14521 tcttaatgtt gtctatattt ccttttttga tatttacaat aagttctata gcttatatac
    14581 caattcttca tttaaataag tatatagctt tgtttagaaa tatgatgcca gaaggtgctt
    14641 ttgctgtgtt atcttcaatt atagtttctg caatagacaa tagaaacctg aaatttttag
    14701 cagtcagttt tgtacttact atgtggacat tttcaagagc ggtaaaagct cttattaaag
    14761 gaatgaatag agcttataaa gttaaagaaa ctaggtcttt tttaaaaatt ttatctatat
    14821 catttttatt tacaataatg cttttggtac ttattttttt atctatgata tttctagttt
    14881 atggagaaaa aataggttat tttattttta accttatagg attggatgaa atatttataa
    14941 aaatatggga tattttaaga tacactgtag gtataataac aataatagtg atatttacac
    15001 ttttatataa atatactcca aataaaaagt tgactattaa agaatctgcg ccaggtgcaa
    15061 tctttgctac ttttgcttgg tttttagtat cgtttttata ttcttattat acaaattact
    15121 atgctaacta tgaagtaatc tacggaagca tagccgaaat aattgtactt atgacctgga
    15181 tgtattttag tagttggtct atcgttattg gctatgaggt aaattcaaga ttgtatttta
    15241 gaaagataag acatgaaatg ttaaagtaaa gtattaaaat aaaagatagg atttaatatt
    15301 ataaatgtaa ttgaattaat aaagaaatat tgacatttaa gttaaaaagt ctagaatttt
    15361 tctggacttt ttttattttt agtataactt gacaaacttt attttatgag ataattttaa
    15421 tttataatca tctttgaaaa gatgactatt gataagtaag agataaaatt ggtagaagca
    15481 tcaagaaata tactaaataa aaattatagt cctaatgagt taatgtctgt attttcaaat
    15541 aatgcagtat ttagtcatgt gtttttatat gcgcctgtgt tcctaattac caatatatta
    15601 gaataagcta ctgtaattag atttagaaaa aagcgacata aataattata aacaaataat
    15661 aaattacata aaattaaatt tattcacaag tatatcttat aaaatatgtt ataattaata
    15721 gaaaattgtc atataattaa ttaagaagta ttattataaa taacattgaa aggtagtggt
    15781 tagaatatgg atacccataa taaatatgta aattttataa aaaatatacc tgtacctttt
    15841 ttatattgta gaattgtaaa gagacaagaa gatatagaat atcgagtaga atatataagt
    15901 aagggtatgg gcaaagtatt acaattagaa gaaggtattt gtgataaaaa tatactagat
    15961 gtattgcctg tatttaagtc taaaaaatat ttcaaagagt tgttctcaaa tgaagtggat
    16021 tgtataaaaa ggtacattcc gacacttaaa aactggataa atataaaaaa acagataata
    16081 ggggattcat acataatttt gtattttggt aaaattgtat ttgattatag acagataatc
    16141 gattcctttg ataaaaagga aaaagtagct tatattaaag acgaagaagg catatatata
    16201 gattgcagtg aaaatttaat accaatatta aataacaaca ttaaaacaac gaaagatatc
    16261 tttggtaaaa atgatataga agtatgggga gagaatacag gaaaattatt tagagatgat
    16321 tatagagaag gagtatctag taaaaaaaga tttttacaaa atctttttga atatgaagaa
    16381 acatttttta tggttgaaaa atattttttg tatgatgaag acgaactttt aggtactata
    16441 gggatagtag acaatataat atatagtggt tacagtaata gaaactataa ctcaaaagat
    16501 ttaatgaaga tgatagagca ctcaattcca gagaatatgt tttataaaga tgtgtatgga
    16561 aattatattg gatttaattc aggttttttg aatttagctt gtatgaataa agaagaatta
    16621 ttagggaaga atagttataa aatatcagaa gaagaagctt taatagataa gatatttgaa
    16681 agtgataagg gtgtaattga aaataaaaaa gtagttacat ttgagttaaa tgtgagtata
    16741 aatgatgaaa ataagtgtat tgagattaca aaaaggccgt tttttgatag ctatggaagt
    16801 gttattggaa taattggaac agcaagggat ataagtagaa gaaagagatt agaagaggaa
    16861 atggataaaa ctaggatgga attttttgca aatttatccc atgaacttag aacacctata
    16921 aatttaatat catcttcatt acaagtgata gaaaaaaaag aagcagattt aatagaatca
    16981 aatgatactt taaaaagaaa tttaggtata ataaaacaaa atggaaatag aatactgagg
    17041 ttagtaaata atgttataga ctttacaaaa atgcaatcag gatatttaga ttttaaacca
    17101 gaagaatctg atataattgc atttatagag gagatatgta tgtctgtagc tgatttcgca
    17161 agtcaaaata atatacaact tacatttgat acagaaatag aagaattttc aatgttattt
    17221 gattcagaaa aattggaaag aataatatta aatttacttt ctaacggaat taagtacaat
    17281 aaggaagatg gaaaaataaa catattttta tatgtgaaag ataatatatt taatatgaaa
    17341 atatctgata gtggtatagg tataccaaaa gaaaaaatag ataaaatatt taataggttt
    17401 gaacagattg acaatgaact ttcttacaga gttaaaggaa gtggcattgg actttcatta
    17461 gtgaaatctt tggttgagtt gcatgaggga agtatatctt taaaaagtca acttggtatt
    17521 gggagtgaat ttatagtttc attacctgtt agaagtaaaa ataatattga aaaatataat
    17581 cataaaagag aaatcagtaa tgaactaagt aaaaaattag aaatagaatt ttcggattta
    17641 taagtttaag tataaaaata aaattttaaa aaagtattga caattgattt agaagggttt
    17701 aaaataaatt acaacataat aaataaaaag gcaatgaata ggaagagtaa atctaattcg
    17761 attaccagag agaagagtcc attggctgga agacttttta aattagaggg attgaaaact
    17821 acctaggagc tactttctga agtcatctgc atcagtagat tttagtaaga atagtcgtta
    17881 gctgcgttaa agtgtaaagt gagttttcat gatatatgaa aactaaatta ggtggaaccg
    17941 cgggaataat actctcgtcc ttatgttata aggatgagag ttttttgtta tttaaaaata
    18001 taaacagtaa tcataataat tatagaaaat ataaaagcga tgaataggaa gagtaaattt
    18061 aattcgacta tcagagagga aagttcacag gctgaaagac tttctaagtt tgagaaattg
    18121 aaaactacct aggagctgtg gtctgaagtt actttaaaaa gttttagtaa gactaaccgt
    18181 taactgcgtt aaagtgtaaa gtggatttta tcaataaatg ataaaatcaa attgggtgga
    18241 accgcgagta tagactcgtc cctttatata gggatgagtc tatttttatt gcaataaaag
    18301 attgaaaata aaaaaaaatc ggaaaattat aaatttagga ggaaaataaa atggagaata
    18361 aagtgaaaat tggagtacta ggatatggag ttgtaggaag tggacttata gatataatag
    18421 ataataataa agaaaaaaga aatatagaaa tagttggaat tttagttaat aatttggaaa
    18481 gacataagga taaaaaatat tctaatataa taactaataa tatagatgat atatttaata
    18541 aagatataga tatacttgtt gaagtcatgg gtggtttaga accatcatta tcttatataa
    18601 aaaaagcatt aaacaataaa attcatgttg ttacagcaaa taaagatttg ttagctgaat
    18661 gtggagatga gcttgcaaaa ttagcaagtg aaaataaagt aagtattaaa tttgaagctt
    18721 cagttgcagg aggtatacca gtattaaaac caataataga atcactagaa ggaaataata
    18781 tagatagtat aaatgcaatt ttaaatggaa ctactaattt tatattatct aaaatgtatg
    18841 atgagaatct atcttatgat atggcattaa gacaagcaca agaattaggt tttgctgaag
    18901 caaatccaga gtcagatgta ttagggtatg atgctgctag aaaactatca atattatcaa
    18961 ctctagctta tgacaataga gtatattgga aagatgtgta tttagaagga attacagata
    19021 tagatgaaaa agatattgaa tacgcaaaaa aattaaattg taaaataaaa ttgattggtc
    19081 aaagtaagta tgaaaatgat aaagtaagtg cttttgttag acctgtttta gtagaaaaag
    19141 ataatatcct tgctagaata gacaatgagt tcaatgctgt catagtgaat ggagattcag
    19201 taggagaagt ttcttttgta ggtaaaggag ctggaagttt agcaacagga agtgcggttt
    19261 attcagatgt tatagatata atagataata gagtttcaag tatagattct tttactaaag
    19321 ataaaataca agttaataaa atagttagag aaaagtgtgg agcacttctt agatttaaaa
    19381 aatgtaataa agatgaaatt ctaaatatag ttgagaattg tttagtaaaa ttcgatattt
    19441 taaatgatga tgatgaactt gctattatgg tttatgctga ttctgaatat gaaataaaca
    19501 acagtttatg tctaataaaa gataaaggat actgcgaaaa aatgaataaa atgttgaaaa
    19561 taagttaaaa aagaaaaaat ataagtttat atgtaacata ttttgacgat tttaatacta
    19621 tatagtatga gatgaaacaa aacatctcat aaaaaaatta aaattaaagg gagagatatg
    19681 ttatatggaa aataaaaaat gttattcaga agattggtat gaaagaggag aatctacagc
    19741 taaatggttc caaaatgata gagaagaata tgaaagagca gcatacgatg aagatagaga
    19801 aagaagaggt tcaaactgtg gatgttcaga ttcaggagaa aatagaccta gaaactgtga
    19861 aagatttaga agagaagctg agataagaga aagagaagca agagaagcat tctgtgaatc
    19921 ttcagagaaa aagaaagaag cattagcata tgaatgtgaa gctagaaaat tatgggaaga
    19981 agcagaaaaa tactgggatg aatattcaaa atacaactat caaggaatcg aatatttagc
    20041 agaagctgct agattatttg atgaaggtat ggaatgtgaa gctagaagaa atggaaataa
    20101 tggaggaaac aataataatt gttgccataa atgccataaa tgtaattgta actgctgtag
    20161 aaaataaaaa aattcattga caaatcaata gttaaaatat atcatattat gtataataaa
    20221 gagaaaattc agaaaaaaat aatgattaga agaaatataa gtatatttat tagacaaata
    20281 gtgctcagtt agtgaggtga gtacaatatg attaaactcc catcctctag cttttgatag
    20341 agggtgggag tttttattta ttgatatatc ttaaatgagg ataatattgt aaaataaatg
    20401 agtatgatat tgtaaaatta ggaggtaaaa aatgaagagt attaaaacat caagtatgaa
    20461 agaaaaggta gatgaaaaga tattaagaga gcaagtaagt tctataatag aagatataag
    20521 aaataataag gatgttgcac ttaaaaaata taatgagaag tttgatagaa atacaagaga
    20581 tgaatttaga attactaagg aagaaataaa ggaagcttat aagcatgtag atgacgaatt
    20641 tataaataac ttaaagattg ctgccaaaaa tataagagaa tttgctaaag ctcaaaaact
    20701 aagttttgaa aacccttttg aaaaagaaat ttatccaggt gtaatcttag gtcaaactaa
    20761 tattccaata gagtcatgtc ttgcatatgt tccaggaggt cagtatccat tgttttcaac
    20821 agctcttatg cttataatac ctgcaaaagt agcaggagta aaaagaatag tggcatgttc
    20881 accaactatg aaaaatactg aaaagataaa tcctaagaca ttggtagcta tggatatagc
    20941 aggtgctgat gaaatatatg caactggagg agttcaagct atagcagcat ttacatatgg
    21001 aacagaaaag attaatccag ttgacataat tgtaggacca ggaaataagt ttgtaactga
    21061 agctaaaaga caatgttatg gacaagtagg gattgatttt gtcgctgggc caagtgaagt
    21121 tcttataata gcggatgaaa cttcaaatcc agtttatatt gcagctgatt tattagcaca
    21181 atgtgagcat gatttaaatg caagaggaat attattgaca aatagcttgg aagtagcaca
    21241 aaaagttgaa aaaaatatag aaagtatgct aaaagatttg ccaacaaagg acatagcata
    21301 tagttcatgg gaaaataatg gagagataat attggttgat gatatggaag aagccattaa
    21361 aataagtaat ttttatgcac cagaacattt agaagttgca gtaaatgagt ataatgatat
    21421 ttgtgataga ttaactaact atggttcttt atttattgga aatctttctg ctgaggtgtt
    21481 tggtgactat gtttcaggta ccaatcatac attaccaaca cttaaagcat caagatatac
    21541 tggtggtgtt tgggttggaa cttttatcaa aacttgtaca aagcagatat ttaatgaaaa
    21601 agctatacaa tcattagcac ctgtagctga aaagctggca aaagaagagg gattatatgc
    21661 acatgcaaag gctgctgagg ttaggtttaa aaagtagtaa aaaattttat tttggcagtc
    21721 gagacttagt attagactga gataaggaaa acgctgagaa cccgaataaa tactatattt
    21781 tgtgggcctc ggcgtttctt ttatatctgt atataataga ataatactat ctgtgtgttt
    21841 aaatactaac ttaatatttc ttttctaaat aatgtttatg aaatagttaa tatttttagt
    21901 taatagttga aaaaaacttt tgctaactgt gcaaattcat ctgcatttgt caatatagca
    21961 gggtgtcctc cctgactaaa aatgtgaata cttgcttttg aaataaaagt tgacatttgt
    22021 ttgtactctt cttcaagatt atgacgacac atttcgtctt ccttacttcc tgtaaataaa
    22081 attggcattt ctaatttaca caattcttta tgaaacaaag ggcgtttctc gtttgcacat
    22141 tgcaatagag cttttgtatc aagattaaca acattctccc aatcttttcc atgacaccat
    22201 tcatagaatt ttcgtgtttg aatatcttgc tttgattttt cccttccagt gattagatta
    22261 tctatgaagt tatcattaag agtacgtcca tcaaagctat ctgctataat tgcttcaact
    22321 aaatcgggac gttctaatcc agtattaaca gctgcccatg ctcctccact tgttccaatt
    22381 aagccaactt taccacaatt caaatgttca atcagtgcta ttgtctgtaa ggcttcatca
    22441 tgccatatgt caggagaaaa ttgagaaact ctatccgact gtccattacc taaaaaatca
    22501 attaagatac atctaaaatt ttctgcatat aatggcacaa gtgctttaaa catattagaa
    22561 gaaccagtat taccatgcag gaaaattatc ggtttacctt gtccatattc tttataaaaa
    22621 caagaattac cttgatactg aaaatatgac atatattttc ctccttttta tttgttaagt
    22681 taattataac aatataggta tgaatgaaaa acctcatgtt ctttcttaga ataaaaatgt
    22741 gccacgcata aaacgtgaca catttctaat tataaatttt gctgtttaag tattctttgt
    22801 atgcttttct ctgtcaaaaa gtatttctct gataactgct tgatactcat tccattttgg
    22861 aaatttatac agatttgaga attacgagaa gctaagactt gtttggtgtt tgtgttttca
    22921 ccccaatgct ttttgtgctc ttgctttttg ggaatataaa tcactttacc atctatatat
    22981 tcttgcactt gctcaatcag ttctttaggc agtaagtcaa tcgaattttg atagctcatt
    23041 ttgcacctcc taaataatta ttctttagga ttagcaaagg ctactgccac tcacaattaa
    23101 aaataaaatt ttttattatg tacaatagcc tttgctatgc acaagttcca attcccataa
    23161 actaaatact cctttctggt ttgaaaaatt catctgaaca ttgatttcgc ttttaagata
    23221 gagttcaatg acaaatttac atttttatta tatatattca gatagataat tgcaactgtc
    23281 tttgtatgag gatattttat tatagtcaat actataatat gtatttatat catcaaacta
    23341 atatgaacag ttgaaaggaa tatgaacaat aaaacttagt caggaagaat aattatgaag
    23401 cagacaattg agaaatttga ttttaaagtt ttattgaaaa tttattgaca aatttaactt
    23461 gtaaatatta tactaaactt aataaataaa gtttataaag taaggtgttg tctatgaaaa
    23521 atatagttgg aaaaccgcaa acgataaaaa aagtaaatga agatttaata aaaaatatta
    23581 taaaaaatga aggtcctata acaaaaccag aaatttcaaa cattactgaa ttgagtttag
    23641 ctacagttaa taagattgta gagcaattag ctttgaaaaa tgaagttaaa gtaagtggac
    23701 taagtgaatc tactggagga agaagagctc aattatttga aataaatgca aacttagaac
    23761 acataatagc tttatattat tatagaaatt gttgtatagg ggtagttgcc aatttattag
    23821 gtgaaataat ttatcaagaa gaatttaata taagaatgga tacgtatgaa aacgttaacc
    23881 aagatacttt ttttgttata gatactctaa taaaaaagga aaaaggaaac aatataaggg
    23941 ctattggaat tggtgtccca ggagttgtaa aaaaaggtgt agttaaaaat ataccaacta
    24001 taaaaagttg ggaaggtata aatttagaaa atttaataaa gagtaaatac aatactaaag
    24061 tgtttataga aaatgataca aatattacta ctattgggat ttatcaaaaa cagtataaag
    24121 acaaatataa aaatatggct cttatgtatt tagaagatgg tattggttca ggtatagtta
    24181 taaatggtga attgtattta ggttctacaa attttgctgg agagttaagt tatctaaata
    24241 tagaatttgg taagtataac tctaacaact taaatcttga agattatatt atgagtttaa
    24301 gaaaaagata tttagaaagt aaagataata aatttaagaa agaaatttta tctataatat
    24361 caaactttat aaggactctt gtatgtgtcc taaatccaca agctatgatt atacagtgta
    24421 gtatattaac caagagtgac atggagttta tagaaaaaga tgtaagcaat aatgttggag
    24481 aagaaaattt tcctaaatta ataaaagtag agtcattgag agaaggaagt ataaatggag
    24541 taattagtat gtgtttaaaa gaaactgact atcaatattc cttatctaat aaaaaaggag
    24601 gatattaatg tgaaatattt ggtttcaata gatggagggg gaacaaaaac taaattttgt
    24661 gtaagtgact tagatggaaa catacttaaa gaacatacca ctggttcaac taattataag
    24721 tcagttggaa ttaaaaaaac atatgaaaat attaataatg gatttaaaaa aatattaaaa
    24781 gatttatata tcgattatga cgatattgag tacactgtat ttggtatatc tggttgtgat
    24841 tctcctaatg attataagat tataatggat gaaatattaa aaattgggat taacaaagaa
    24901 aaaatatatc tggcaaatga tgcagtttta gctttttatg cacaagctga ttcccctggt
    24961 ttagtaattg tggctgggac aggttcaatt atactgggaa ttaaagaaga tggagaaata
    25021 tatagagttg gtggatgggg gtataatttt agtgatttgg gttctggata tgatataggg
    25081 agaaaattat taaagaaagt attgctttat tgtgatgaat gtcatgagta tagtgattta
    25141 tttagttgtg tcttggattt ctttggagca aatagttttg aacaacttgc atatatgatt
    25201 actgatataa atgataatgt tgaaatagca aacttagctt cattagtgat tgattgtgcc
    25261 gaacaaggtg ataaattggc tattgaaata ttaagagaat catcaactga actatctaaa
    25321 ttagctcaag taatattgag aaaaatatct aatccaatga aaatggataa aagcgaaata
    25381 aatatagttt tgtctggtgg tacattaaat aaaactgtat atgctcaaat gttaatgaat
    25441 aatttaaaaa cagaaaatac agataaaaat ttaaaattta tatctcaaga aaatcagcca
    25501 gtatatgggg gataagactt gctatggcat tagctaatag gagggtcaag catggataga
    25561 aaaaaggtaa tagtacaaat ttttactgga ggttttaagg ctaaagaaat aaagtttaat
    25621 gatataaaat gtaaactact agaattattg gactgtattg atatagaaaa ggttatcatg
    25681 ggatggagtg tagatgaaga attatatgta gaaactaaaa aactattaaa taaatataat
    25741 gttgaactat atctttggtt gccatgtttt tctgaaatag gattacttga aaaaagtaat
    25801 ttactaattg attatatggg gaaggaagtc aagggctatg ctttacaaga ggatgaaaat
    25861 tttgagtttt attgcccaaa taataaggaa aatataaaaa atataaagaa aatatatagt
    25921 acttatttta gtgatattaa atttgatgga atttttttag ataaaataag gtatggttct
    25981 ttttcgaata aactaaatgg tgtttttaat tgtttttgta aagattgttt aaatatatat
    26041 gaaaaaaata atattgataa agagtcgtta atgagagaaa tgaataatgt gtctaaggga
    26101 gttatagata atgaagatac tccatttggt ataacagaat acattaatgg aaaatacaaa
    26161 tttaaaaata aaatatggga agattttttt gaaacaaaaa atcaaaatat atacaaggca
    26221 ttattagaga taagtaatta ttttgaaagt aagggattga aaataggtat agatactttt
    26281 tctcctttta tttcttactt tacaggtcaa gatttagaaa aattacaaga agtagcagat
    26341 tttataaaac caatgatgta tagaactaca aaagctccag ctggtttacc atttgaattt
    26401 gaaaggttta ttgatgaatc catacataag aataaagaga ttggaaaaag aaatttctta
    26461 gatattttag gaattaagga gtatgaagga gatgtatacc ctatagaatt tgtactagag
    26521 gaaatcgatt ttatgacaag aaatcttaat aaagatatat atgtaggagt tgaaataaat
    26581 aaaaaggaaa ttatagctcc tgttacacct aagtacataa gtgaaaatat ttcaaatata
    26641 aataagacaa cagtaaaagg atatgtttta tcttgggatt tgttatcagc accaaaagaa
    26701 aatattgatg aaattataaa atggtttcgg aattagtgag gtgatatagt gagtaagata
    26761 ttggaaatgc aacaaatata taaaagtttt ggaaaggtag atgttttaaa agggataaat
    26821 tttgagttag aaaagggaga agtaagagct cttttgggag ctaatggagc aggaaaatct
    26881 acacttatta aaattttagg aggagttcat tcccaaacta aaggtaaagt aattttgact
    26941 ggtaatgaaa taatgtttaa aaatccacat cattctaaag aaaatggtat tagtataatt
    27001 tatcaagaat taagcttgat accaacgctt acagtacttg aaaatatttt tcttggtaga
    27061 gaaattttaa aaggaagtgt gtttttaaac aagaaagaca tgcttgagga atatatgaaa
    27121 atttgtgagg attttgattt taatatagac ccacatatag tagtttctaa tttaagtgta
    27181 gcaaaccaac aaatggttga gataatgaaa gctgtttcat gtaattctga aattatcata
    27241 atggatgaac ctacaacatc acttactaat aatgaaaaag atgggctatt taaaataata
    27301 agtagactaa agtctaaagg taaaacaata gtctatattt ctcacatatt agaggaagtt
    27361 tttaaagtat gtgatagtgc aagtataatg aaaaatggaa ttatgattgg aacttatgat
    27421 gtagaggatt taactaaatt aaagataact caacttatga caggaaatga taatcacaaa
    27481 cttattgaga agaaggatta tacatttgca aaatatgaag agccaccagt gctagaagta
    27541 aagaatataa gctggtctaa caaaataaaa aatgtttcat ttaaagttta tagaggcgaa
    27601 gttgtaggtc ttgcaggtct tgtaggttca agaagaactg aaattataaa tttgatattt
    27661 ggtatagata ataaagatag tggagacatt tttatcaatg gtaaaaaagt agacataact
    27721 tctcctaagg ttgctataaa aaacaaaata ggattgattc ctgaagatag aaaaaatcta
    27781 ggattaattt tagaacaagc tatatacaca aactcatcat cagtacaaat tgataaattc
    27841 aagaaattct tgtttataga tagaaataaa gaaattgagt tttctaaaaa aggtgttaag
    27901 gaattaggaa taaaagttag tgatgtaaag caagaagtaa aagagcttag tggtggaaat
    27961 caacaaaaag tagtagtttc taagtggtta gagaaagatt tagatttaat aatatatgat
    28021 gagccgacaa agggaataga tattgctgca aaagaagata ttttcaatac aatcaaagaa
    28081 ttttcaagta aaggaatagg agtaatattt atatcttctg atttagaaga agtaataaga
    28141 gtaagtgata gaattttagt aactagagaa ggttctattg tagatgattt aaaaaatgag
    28201 aatatacagg ttcaagatat aatgaacagt atatttaatg tataaaaaat gaaaatggag
    28261 gactgagatg aagaaagctt ttgagaagtt aaatttaaga gattatggag tattattagg
    28321 ttttttagct ttatgtatag ctataagtat tgcaactcca tcatttttag gtaaacaaaa
    28381 tatacttaat cttttacgac aatcttctat aattggaata atttcagcag gtatgacttt
    28441 tgttataata tctggaaact ttgatatatc agttggagca gtagcagctc ttgcaggtgc
    28501 tataacaatg aaatttgcga ctggtggaac taatttgttt gcatccatgc tcttaggtgt
    28561 agcgatttgt gcagtaatag gattaatcaa tggtatagta gttgcaaaga taaatgtacc
    28621 atcattaata gccactatgg ctatggtcac tattgtaaga ggattgctgt tgatgcttac
    28681 aggaggatat ccaattactg aaaatatacc aatacttgat tacattggaa atggatattt
    28741 gtttaatata ccaataccag tagtaatttt ctttatagtg gtgttagttt cgtttattgt
    28801 acttaataaa actaagtttg gtagatatgt ttattctgta ggagggaatc aagaagcaag
    28861 taaacttaat ggtataaatg tagattctca caaagtgaaa gtgtttatta ttaatgctgt
    28921 acttgcagga atagctggaa tcgtactaac aggaagactt gggactgcaa ctgcgattgc
    28981 aggtgaagga tatgatatgg atgcaattgc atctgtagta ataggaggta catcagtggc
    29041 aggaggttct ggttctgtac ttaaaactgt tataggagta ttacttatga gtgttataaa
    29101 caacagtttt aatcttcttg gaatagatgt atatttccag tacatattta aaggattaat
    29161 aattttagca gcagttggat ttgattctta tagcaagaaa aaacttgctt caagataaaa
    29221 aataaggggg atttttttat gaaaaaatta atgaaacatt tagcattgtt gctatcactt
    29281 gttatgatat ttggtttagt tggttgttca aatggtggtg acaaagaaaa aaatggagaa
    29341 aagaaaatag cagtattact accgggttca acaggttact ttgtagcaac taaacaaggt
    29401 atagatgcaa aatctaaaga attaggtgta agcgtggaat atgcagatgc tcaatgggat
    29461 gctagtaaac aattatcaca agctgaagat tttatggcta aaggggtaga catgattgca
    29521 ttatgtggag ttgattctgc tgtaagtgag agaatagtta aagctgctaa tgattcagac
    29581 gttcctatag tagcatttac aaattctata ggaagtaacc caactggaga atttaaaggt
    29641 ttagttacat atataggaca aaatgaggaa gaaacaggag cattaactgg aaaaattgct
    29701 aaaaacttat taggagaaac tggtgggaaa gctgtattaa tagaaggagt accaggaact
    29761 actccacaag tattaagaaa aaaaggtctt gaaaaagaat taaaagatag taatatagaa
    29821 atagtttata atcaaacttc aaattgggag aaagagcaag ctttaaaggt aacagaagat
    29881 ttaattcaaa agaaaactga ttttaatgtt ataatatgtc aagatgataa ttctgctaca
    29941 ggtgcaggtc aagctttaaa agatgctggt cttaaggata aagttaaagt cataggtttg
    30001 ggtggaagta aagatggatt aaaagctata actgatgggt taatagatgg tacaacttat
    30061 atgtctgctg tagaagaagg tggattagtc atagaaaagg catctaaatt ccttaaaggt
    30121 gaaaaaatag aacctgttac acaaattaaa caagttgaag ttaacaaaga taatatttca
    30181 gagttcaagg gtgaatggta aattgtcata gataataaat aagattttta gagttacttt
    30241 agaaactata taaagtaact ctttttttaa tttaatttag tgataaatta ttgaatagta
    30301 tttctaaaca aataaaaaat taatataaac aaagacttaa ttatactaaa ttataagtaa
    30361 aactaatata aacttatgga aaaaaggaaa acgtctaaag tattggaatt actatagtgt
    30421 aggtgtggta ttaaaaaaac ataaaaaaac ataaaaaaat tgttgaaaaa aatatagaaa
    30481 ttttgtataa ttaaagattt gaaaagtgcg gtatacaaag ccaattcttt tgtatataat
    30541 atatataaat tcagaaaaag gaagggatat gtatggccag gcggttattt ttagaaagga
    30601 ttggacttat atttttaggt tcagcaatat tagcatttgg agtttataat ttttattatt
    30661 taaacaacat aacagaaggt ggagtgttag gtattttact actgttaaaa aatttattca
    30721 atatacaacc agcaatagca aatgttgtaa tagatggttt actactttta gtaggatata
    30781 aatttttcgg taaaaaattc cttatatatt caatagtagc ttctataact ttttcagtgt
    30841 tatatgactt atttgaagct ataggacctc tagtaccaca atcacaaaat atgcttttat
    30901 caactatttt ggcaggtgta actgttggaa ctggtgtagg tatagtagta aaagcaggtt
    30961 gtgcatcagg aggagacgat gctttagcat tagtaatttc taagactaca tctttgaaca
    31021 tagggcaagt ttatttagca acagatgtta ttgtattgtt gttatcatta ttttatctat
    31081 cagcgtttga tatcttttat tctttgattg ctgttacaat tagtggcaag gtaattgatt
    31141 ttatttatta tcatggtaaa agtttggata tggatataag taatgatata gttccagaat
    31201 gttaatataa attaaataac tttagatttt attaggggtg gcagatttgc cacctttttt
    31261 gtgtctaaat ctttatagaa agtataatcc tatctaggtt tttgcaaatc tcaaatatag
    31321 aattatgttg aaatgactgg gaatacgatt gctagatgtg cattttaaaa acccaatttt
    31381 atatttatga aatactaatc attaatggat gtttcttaat cctatcttta tacctaaaat
    31441 gataagataa atgaagtagc aatacaaagc taaaatttgt aaaagagatt aaattaaatg
    31501 gaggagctaa aatgttggat atagttatga tagcattgtt attcatagga tttgtttcca
    31561 tgaagttact tgctgattgg tgtgggaaac aaactgaaaa aaaataatgt aatgtatgga
    31621 gggattacct atgtggtttc tggcagttgt aattataatg ttaataatat atttggtata
    31681 tgcgcttatt aatcctgaaa aattttaaac agtataaggc taataagtaa gttgaattgg
    31741 ggggaacaat ttttatggtg cagatagtaa tagtgttagc gatttttatg attttagtaa
    31801 tacctatggg caagtattta tatcatatag ctactaatca aaaaacattt ggagatagac
    31861 tatttgataa agtggacaat tttatatata aggtatgtag tatagataag aaaaaagaaa
    31921 tgaactggaa acagtatgca ttagctttat tatttacaaa tgcagttatg gtatttatag
    31981 gatatatcat acttagaact caaagtatgc atatttttaa tccaagtgga attaaaagta
    32041 tggaacaagg actatcattt aatacaataa taagttttat gacaaataca aatcttcaac
    32101 attattcagg tgaatcagga ctttcatact ttagtcagat gacagtgata atatatatga
    32161 tgtttacatc agctgcaaca ggatatgcgg ctgctatggc atttgtaaga ggtctagtcg
    32221 gtaaaaagaa aacattggga aatttttatg tagatttaat taggataaca acaagagtac
    32281 ttttaccagg agctttaatt attgggctta tattagttac acaaggagta ccacaaactt
    32341 ttgctgggac agaaacagtg actactatag aaggaaagct tcaagatata gctagaggtc
    32401 ctgtggcagc tcttgaatca ataaaacatt taggaacaaa tggtggtgga ttctttggtt
    32461 caaattcatc acacccattt gaaaatccaa caataatatc aaatatagta gaaatattat
    32521 ctatgatgat attaccagga gcttgtgttg ttgcatttgg tcacatgata aagaataaaa
    32581 aacaaggttg ggttgtgttt ggcgcaatgt caatcatatt tttaataggt ctagtagtgt
    32641 gctttaaagc agaaagtgca ggaaatccta ttttatcaca attagggctt aatcaaagta
    32701 tgggaagtat ggaaggtaag gaagttagat ttggtatagc acaatcatca ttattcacaa
    32761 cagtgactac ttcctttacg acaggtacag ttaataatat gcatgataca ctgactcctt
    32821 taggaggatt agtaccactt ttaaatatga tgctaaatgt agtatttggt ggtaaaggcg
    32881 taggtctgat gaatatgttg atgtacgcta taatagcagt attcttatgt ggattaatgg
    32941 taggaagaac accagagttt ttaactaaga aaatagaagg aaaagaaatg aagcttattg
    33001 ctttacttat tatattacat ccacttttaa tactaatgtt ctctggtctt tcagtagcaa
    33061 taccagcagg gcttgaagga atttcaaatc ctggattcca tggattatca caggtattgt
    33121 atgagtttgc atcttcagca gccaataatg gttcaggatt tgaaggtcta ggagataaca
    33181 ctatgttttg gaatataaca acaggtatag ttatgttctt tggaagatat gtgtctatta
    33241 tagtactact agcaatatca agtttgttag catctaaaaa agctgtaaat gaaagtatag
    33301 gaacacttag aacggataat tttacattta caatagtttt agtacttgtt gttttaattg
    33361 ttggtgcttt aacatttttc ccagccttag cgcttggacc tatttcggag catctagtgt
    33421 tgtggcacta aaaaattggg agggagtaaa gttttatgga aactaaaaaa acaaagttta
    33481 ttacaaaaga tattttcaaa acttccatta tagaagcttt taaaaaatta aatcctaaat
    33541 atatgatgaa aaatccagtt atgtttgttg tagaaattgg atttatagta acattgatac
    33601 ttacaatagt acctagtttg tttggtgatg aaggcaataa cttaagagct tataatggga
    33661 ttgtaacaat aatcttattt ataacagtcc tttttgcaaa ttttgctgaa gctgtagctg
    33721 agggaagagg taaagctcaa gcagatacac ttaagaaaac tagaaaagat acagtagcaa
    33781 gggttataga tacgttagga aatgaaaaaa tgataagtgc aagtgagcta aaacttggtg
    33841 atattgtttt agtaaatgct ggagaagtta ttccaaatga tggagaagtt atagagggta
    33901 tagcttctgt ggatgagtca gcaataactg gagaatctgc acttgtcatg agagaatcag
    33961 gtggagattt cgcatctgtt acaggtggta ctacagttgt aagtgactgg ttaaagataa
    34021 gaataacaac aaaaccagga gaatcgttct tagataaaat gatagctctt gtagaaggtg
    34081 cttcaagaca aaaaacgcca aatgagattg ctttaaatac tttgttagta gggttaacac
    34141 ttatatttat ggttgtttta atatctcttt atccaatggc aaactatgca ggtgtaaaga
    34201 ttccaatttc tactatgata gctcttttgg tatgtttaat accaactact ataggtggat
    34261 tgttgtcagc tattggtatt gcaggtatgg atagagtaac tagatttaat gtaattgcaa
    34321 tgtcaggaaa agcagtagaa gcttgtggag acgttgatac tatgattttg gataagacag
    34381 gtacgattac ttatgggaat agacttgcat cagaatttat agcagttgga aatgcagatt
    34441 caaaagattt aataaattat tctgttatgt gttcattaaa agatgataca ccagaaggaa
    34501 aatctatagt tgatttaggt atgaaattag gttcaacagg taagacaaaa gaggcagagg
    34561 aagctgagtt tgtagaattt tctgcacaaa ctagaatgag tggtattaac ttagctgatg
    34621 gtagagctat aagaaaaggt gcttatgata gcataataaa aagagtaaaa gaatctggtg
    34681 gtacagtgcc agaagattta gaagaaaatg taaatcgagt agctaaacta ggaggaacac
    34741 ctttagtagt gtgtgtagat aatgagatat atggaattat ctacttaaaa gatacagtaa
    34801 aatcagggct tgtagaaaga tttgctagac ttcgagagat aggaataaag acaataatgt
    34861 gtacagggga taatcccttg acagcagcta caattgcaag agaagctggg gtagatggat
    34921 ttattgcaga atgtaaacca gaagataaaa tagatgcaat aaaaaaagaa caattagagg
    34981 gtaaaatagt tgcaatgaca ggtgatggaa caaatgatgc tcctgcactt gctcaggcag
    35041 atgtaggtct tgcaatgaat agtggtacaa catctgcaaa agaagcagca aatatggtgg
    35101 atttagattc agacccaact aagatattgg aagttgttga aataggaaaa cagcttttaa
    35161 taactcgtgg ggctttgaca acatttagta tagcaaatga tgttgcaaaa tattttgcga
    35221 ttataccggc tatatttact cttgcaatac ctcaaatgga gatattaaat gtaatgaaac
    35281 tttctacgtc atttagtgcg atattatcag ctttaatatt taatgctata attatcccat
    35341 gtttaatacc tcttgcaatg aagggagtaa aatatagacc tatgaaatca caagctatgt
    35401 taatgagaaa tatgcttata tttggtcttg gtggagttat agtaccattt ttaggaataa
    35461 aattaattga tttattgatt acacctttag taatggttct taatttaggt taataaatgg
    35521 aggatataaa aatgaaaaca ttaaagccag cattacttgt aagtattgta ttgttagttg
    35581 tttgtggact tgtatatcca cttgttttga caggtgtaag ccaagttgca tttaaagata
    35641 aagctaatgg aagtatgata gaagtaaatg gtgtaaaagt aggttcagaa cttattggtc
    35701 aaagctttac agatgcgaga ttctttaagg gaagagtatc atcagtaaac tataatactt
    35761 atacaaaaga agatttagta ccagataaag atggaaatac atcttatgga ggtgtatcat
    35821 cagggtcttt taattatgga gctacaaatc cagaacttca tgatagagta caaaaagata
    35881 tagaacagtt tttaaaggat aatccaactg tcaaaagaga ggatatacca acagatttac
    35941 ttacagcatc aggttcagga cttgacccta atataagtcc agcctctgca aaaattcaga
    36001 taccagctat agcaaaagcg tctggaatat cagaaagtaa attacaaaaa attgtagatg
    36061 ataatacttc caaaaaactt tttggagttt taggtgaaga tagagttaat gtattaaagg
    36121 ttaatgtaga ggtcgcaaaa atattaggtc ttatatagta ttttgtggaa ttttagcaac
    36181 aaaatattac gcaagattta atttaaaggt taaattttaa agagtgaaaa tcgataatga
    36241 ttttcacttt tttatttgaa tattactaat atttggaata aagtgattgt aagtataaaa
    36301 tccaaaaaaa ggttgcatta tcaaaatgat aatactataa tatatttatc gttatcaatt
    36361 tgataataga tttggacata taggaggaaa tagtatgtta tataataacg cattagagtt
    36421 gataggaaac acacctgtag taaggctgaa taatttaggc tgtactaatg ggtgttctaa
    36481 catatatgta aaattagaaa aagtaaatcc agcaggaagt ataaaagata gagctgttta
    36541 tgaaatgtta gagggcttag agcaaagagg agaattaaaa aagggagatg tactagttga
    36601 agcaactagt ggaaatactg gtattgcatt atctatgata ggaaaattaa aaggatatga
    36661 agtagtaatc gtaatgcccg aaacaatgag cgttgagaga agagatttaa tgaaggctta
    36721 tggagcaaat ttggtgctta cagatggtaa agctggaatg gcaggttcca tagaaaaagc
    36781 aaatgaactt ttaaaagaaa atccaaacta taaaagccta aaacaatttg aaaataaaga
    36841 taatccaaat gcacattata aaacaacagc agttgaaatt atgaatgatg tcaaagattt
    36901 agatatattc gtatgtggag ttggaacagg tggtacttta ataggaactg caagatattt
    36961 aaaagaacaa aatccaaata taagagtaat tgcacttgaa ccaaaaaaat ctccagctat
    37021 atcagaaaac aagactggac ctcataaaat acaaggaata ggtgctaact ttgttcctga
    37081 aaattatgat tctagtgtag ttgatgaagt aatattagta gatgatgaag atgcatttga
    37141 tacagttaaa ttacttgcag caaaagaggg aattttagta gggatatctt ctggagcaaa
    37201 tatatttggt gctatagaga tggcaaaaaa gtatccagat aaaaagatag ttacagtagc
    37261 tccagatgga gttgataagt atatgtctat gggaatattt taataatttt atttgaggga
    37321 gtgaatactt tgtttaagaa aataaataaa gatattgaat atataatgaa gaatgatcca
    37381 gcagctaggt ctaagataga agttttttta ctttatccat cagttcatgc aatgataatg
    37441 cataggatgg ctcatgctct ttataagaag aagaagcttt ttacagcaag attaatatcc
    37501 cagatatcca gatttatgac tggcatagaa atacatcctg gtgctaaaat gggggaaggt
    37561 atattgatag accatggaat gggtgttgta attggagaga cagcggaagt agggaatagg
    37621 gtgacaatat atcaaggagc tactttagga gctacaggaa aagatactgg aaagagacat
    37681 ccaactgttg gtgatgatgt attaattggt gctggaacaa aaatactagg acctttaaat
    37741 ataggttcaa attcaaaaat tggtgcaaat tctgttgtcg ttaaggatgt acctaatggt
    37801 gcaacagttg ttggtatacc agcaaaaata gtaaaaataa gaaacctaga acctgtaaaa
    37861 aagaataaaa aagaagtgtc ctatgagtat gatgaattag ataatgtata ttatatatag
    37921 gatttatata actataaaat aaactttata aaaattattt taaaaggtat ttttttcaaa
    37981 tttgaggaca taataaaaaa gaactgaatg ttacagtttt ggttcatcaa tctttgataa
    38041 acaagatttt aaatttgtag cgtctaggag gattttatta tgaaagcaaa tgttaatcaa
    38101 gatacttgta taggttgtgg attatgtcca tcaatatgtc cagaagtatt tgatatgaag
    38161 gatgatggga aatcacatgt tatagttgat gaagtaccaa gtgatgctga agaatcagca
    38221 gcagaagcta gagaaagctg tccagttgat gcaatagatg tgcattaata aaataaatgc
    38281 ctataaaaat tgaattttca atttttatag gcatttgtct tttattcagt tagtatttca
    38341 acacctgttt ctgtaactaa aaccatacat tcccattgag cagataattt accatctttt
    38401 gtataaatag tccaattatt ttcttcatca acaaaaattt cgtgagttcc ttcatttacc
    38461 ataggttcta ttgtgaatat catcccagga accaatagca tctcagtccc ttttttagat
    38521 acataactta caaaagggtc ttcatgaaag cttaatccaa ctccatgacc acctatttct
    38581 ctaacaacag agtatccatt tttctttgca taagagttga tagcatcacc tatatctcct
    38641 aaaaatcccc atggtttggc aacttcaaga cctttttgga cacattcctt tgtaacttga
    38701 actaattttt ttgcattttc attaacatta cctatcataa acattcttga agcatctgaa
    38761 tagtatccat taaaaatagt agaaacatct acatttataa tatctccatc ttttaagata
    38821 atgtccttac ttggaatacc atgacaaact tcattattta ctgatgtaca tacactttta
    38881 ggaaatccat tatagtctag tggagcaggg atagctccat tttgtagtgt aaagtcatga
    38941 accaacttat ttatatcttc tgtagacatt ccaatttcga ttttatctga aatataatcc
    39001 aaaatttttg tgtttattac agcacttttt tttatttcct caatttgact ttcatttttt
    39061 ataatatcat gagttggtac aatatgacct aacttagcaa atgaatctat tttattatcc
    39121 atatccatat gacatttttt atatttttta gtacttccac accaacagct atcatttcta
    39181 tttaacttca taacttttca tccttctttt attatttatt taaattatgg gttacataaa
    39241 ctattttaaa acataaccat tataaatatt ataacataaa tactaagaaa tcctttatag
    39301 atagtgattt attcaaagta taaattatta tatggttatt gttgatgaaa agaggatagt
    39361 taaatgtatt attggatatt agcgcactta atagtttagt tcaagttttt caagaaaatc
    39421 atatgttaga ggacaatttt cctttaaagt gttttttgaa tcttcatcaa gataataatg
    39481 cacaaatgat tctgcaaaaa attcttctat aaacttgctt atatatgcat gtgcaacttg
    39541 cttagggtca taaagcttat atctttcctg agcaaatact tccataaatt cttcagaatt
    39601 tgatttttta tttaaaacat ttttatctat agcatgtgca gtttcatgta attctaaatt
    39661 ttttgatgaa tgtacatttg catagctagg gttactataa cctattttag ccactataga
    39721 attatttcta tatataccag gtacatcttt ccaagtcttg cctgatctta cccaacatgg
    39781 aggtaattga tattttaaat cactaaattc tggttcatca gttagatttg aatttattaa
    39841 ttttattttt atatcctttt ctttaagtgt ctgtaaatat ttaatattta tttttgcaag
    39901 tctgtttttc atattttcaa cttcatcttt atcataattc aagtcatcag gaagtataag
    39961 catttgttca attatatcta tactactgtc ttttatattt tcagattctt catcaaattt
    40021 atattgaaga gtattatttt catagatagt tatatttgta ttattttcta tagaataaga
    40081 gtgaatatcc tctaaatcta aaatattcat ttgttgtaaa atatgttttg tttcgccact
    40141 ccacactact aaagaaagtg aattaaaatt aattagtgga ttttcttttg cttttatatc
    40201 attgcttata tctacatcaa atacatattc aattgagtct tctatgttaa gactatctgt
    40261 attcttttta atatataaat acgcttgttt taagtctata taaatctcat ctaaagattc
    40321 gtcataaaat tctgataaat tattagtaaa gagttcttca cttaaaagtt catttttatc
    40381 agttatactt tcaattaaaa tattaatttc ttttatcata tcgtaaatac ttatttttct
    40441 atttgctttt tcactagata cattgcatgg cgcaagagca atgcaataaa aatctttttc
    40501 ttttttataa aaggctttaa gtgattttga taaaagtgta aagtctacac ctatctgtaa
    40561 gttgacctta agtgagtcat tttgtaataa ttcagtatta tttcttttag ctgtatttaa
    40621 attgttttcc ataatcattg cccccttttt attagggtat cataattttt ttatatgtgt
    40681 caatttgttt ttttgaaaat tataattttt ttgaaaattt tattttttat aatttgtgaa
    40741 ttttttatta tagaaataaa tatgttatta ttatgtcaaa atttagcatt agattcatgt
    40801 agagtatgtt ttatacatat attataatta attctttgaa gattgcatat tcaaaataca
    40861 caccattttt t
//
LOCUS       DAEORZ010000020        40177 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEORZ010000020 DAEORZ010000000
VERSION     DAEORZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40177
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.020"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(38..277)
                     /locus_tag="KRH39_000930"
     CDS             complement(38..277)
                     /locus_tag="KRH39_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8510851.1"
                     /translation="MNYNLIKVIFSLFKKKSGYTFTNFLNKVRVEKSKKYILKKDLSI
                     LDVAVLIGFNSQNYYSIIFKKFNNFTPIEYKNIYN"
     gene            548..739
                     /locus_tag="KRH39_000931"
     CDS             548..739
                     /locus_tag="KRH39_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510852.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(828..1640)
                     /locus_tag="KRH39_000932"
     CDS             complement(828..1640)
                     /locus_tag="KRH39_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBG8510853.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            2062..3213
                     /locus_tag="KRH39_000933"
     CDS             2062..3213
                     /locus_tag="KRH39_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8510854.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLESLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            3331..3969
                     /locus_tag="KRH39_000934"
     CDS             3331..3969
                     /locus_tag="KRH39_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG8510855.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            3970..4992
                     /locus_tag="KRH39_000935"
     CDS             3970..4992
                     /locus_tag="KRH39_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBG8510856.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLDTLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            4982..5602
                     /locus_tag="KRH39_000936"
     CDS             4982..5602
                     /locus_tag="KRH39_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510857.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKVGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(5726..6412)
                     /locus_tag="KRH39_000937"
     CDS             complement(5726..6412)
                     /locus_tag="KRH39_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBG8510858.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKEYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDILGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(6426..6971)
                     /locus_tag="KRH39_000938"
     CDS             complement(6426..6971)
                     /locus_tag="KRH39_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8510859.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            7199..8446
                     /locus_tag="KRH39_000939"
     CDS             7199..8446
                     /locus_tag="KRH39_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG8510860.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            8533..10527
                     /locus_tag="KRH39_000940"
     CDS             8533..10527
                     /locus_tag="KRH39_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG8510861.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNYCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            10527..11849
                     /locus_tag="KRH39_000941"
     CDS             10527..11849
                     /locus_tag="KRH39_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG8510862.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            12128..13414
                     /locus_tag="KRH39_000942"
     CDS             12128..13414
                     /locus_tag="KRH39_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG8510863.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            13500..14807
                     /gene="grdB"
                     /locus_tag="KRH39_000943"
     CDS             13500..14807
                     /gene="grdB"
                     /locus_tag="KRH39_000943"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:14547..14549,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG8510864.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            15085..15816
                     /locus_tag="KRH39_000944"
     CDS             15085..15816
                     /locus_tag="KRH39_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8510865.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            15818..17176
                     /locus_tag="KRH39_000945"
     CDS             15818..17176
                     /locus_tag="KRH39_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG8510866.1"
                     /translation="MINLFSSDLFLKYSFLFSFIVYRILMVIVQYYFLISFTNVNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            17215..17700
                     /locus_tag="KRH39_000946"
     CDS             17215..17700
                     /locus_tag="KRH39_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510867.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKSLQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(17963..18199)
                     /locus_tag="KRH39_000947"
     CDS             complement(17963..18199)
                     /locus_tag="KRH39_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510868.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTDKIQTAS"
     gene            complement(18354..18578)
                     /locus_tag="KRH39_000948"
     CDS             complement(18354..18578)
                     /locus_tag="KRH39_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG8510869.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(18675..19928)
                     /locus_tag="KRH39_000949"
     CDS             complement(18675..19928)
                     /locus_tag="KRH39_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBG8510870.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            20188..21177
                     /locus_tag="KRH39_000950"
     CDS             20188..21177
                     /locus_tag="KRH39_000950"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBG8510871.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            21249..21989
                     /locus_tag="KRH39_000951"
     CDS             21249..21989
                     /locus_tag="KRH39_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBG8510872.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            22205..23356
                     /locus_tag="KRH39_000952"
     CDS             22205..23356
                     /locus_tag="KRH39_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG8510873.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            23381..24136
                     /locus_tag="KRH39_000953"
     CDS             23381..24136
                     /locus_tag="KRH39_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBG8510874.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            24328..26715
                     /locus_tag="KRH39_000954"
     CDS             24328..26715
                     /locus_tag="KRH39_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBG8510875.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVIFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(26946..27179)
                     /locus_tag="KRH39_000955"
     CDS             complement(26946..27179)
                     /locus_tag="KRH39_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBG8510876.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            27469..28059
                     /locus_tag="KRH39_000956"
     CDS             27469..28059
                     /locus_tag="KRH39_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8510877.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            28173..29018
                     /locus_tag="KRH39_000957"
     CDS             28173..29018
                     /locus_tag="KRH39_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510878.1"
                     /translation="MIKVDDLSFSYTERDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            29015..29698
                     /locus_tag="KRH39_000958"
     CDS             29015..29698
                     /locus_tag="KRH39_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8510879.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            29695..30411
                     /locus_tag="KRH39_000959"
     CDS             29695..30411
                     /locus_tag="KRH39_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8510880.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            30508..30771
                     /locus_tag="KRH39_000960"
     CDS             30508..30771
                     /locus_tag="KRH39_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8510881.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            30743..31198
                     /gene="def"
                     /locus_tag="KRH39_000961"
     CDS             30743..31198
                     /gene="def"
                     /locus_tag="KRH39_000961"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG8510882.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIEQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            31250..31711
                     /locus_tag="KRH39_000962"
     CDS             31250..31711
                     /locus_tag="KRH39_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBG8510883.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            31761..32663
                     /locus_tag="KRH39_000963"
     CDS             31761..32663
                     /locus_tag="KRH39_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8510884.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            33033..33845
                     /locus_tag="KRH39_000964"
     CDS             33033..33845
                     /locus_tag="KRH39_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG8510885.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            34893..35654
                     /locus_tag="KRH39_000965"
     CDS             34893..35654
                     /locus_tag="KRH39_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG8510886.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            35719..36501
                     /locus_tag="KRH39_000966"
     CDS             35719..36501
                     /locus_tag="KRH39_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG8510887.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            36768..37187
                     /locus_tag="KRH39_000967"
     CDS             36768..37187
                     /locus_tag="KRH39_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBG8510888.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(37290..37556)
                     /locus_tag="KRH39_000968"
     CDS             complement(37290..37556)
                     /locus_tag="KRH39_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBG8510889.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            37783..38484
                     /locus_tag="KRH39_000969"
     CDS             37783..38484
                     /locus_tag="KRH39_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510890.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            38566..39174
                     /locus_tag="KRH39_000970"
     CDS             38566..39174
                     /locus_tag="KRH39_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG8510891.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            39498..39962
                     /locus_tag="KRH39_000971"
     CDS             39498..39962
                     /locus_tag="KRH39_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HBG8510892.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
BASE COUNT        16070 a         4100 c         6605 g        13402 t
ORIGIN      
        1 tatgtaaaaa aataacatcc ttttagaatg atatttttta gttataaata tttttatatt
       61 caattggtgt aaagttatta aactttttaa aaattatact atagtaattt tgactattga
      121 atccaattag tacagcaacg tctaaaatag acaagtcttt ttttaatata tactttttgc
      181 ttttttcaac tctaacttta tttaaaaaat tagtaaaagt atatccactt tttttcttaa
      241 acaaagaaaa aataactttt attaagttgt aattcattac atatattact tactgttata
      301 tcttctgagt aatttttgtg aatataattt atagttttct ttaagttttc tttacataaa
      361 actaagagtt ggtctatgtt tcattttatc aaggcatatt tattaaaaaa tcaatctcta
      421 tttgttaaaa taatttatga atgatgttag aaactgtaat atattggata taatataata
      481 aacagataaa ctctgcatta agaggatata tagtttatct taattatagg ggaagtgagg
      541 atatattatg gaaagattaa tggaactcat agaaagaaaa tttttaaagg atgtagaact
      601 tttagaatta gaagacatag aaaatgtaat agaatgcaaa atgacacagc catataatgc
      661 tcattataat gaatatttag taaaagtaaa aaatggagat gaagtagaag aatattttgt
      721 gtatttaaaa catagataaa cataaaagag gtttatattt atttgtatga ttataaaata
      781 aatacaagcc tctttttttg ataaatataa aattataaaa tttttattta tttaattttt
      841 tcaacaacat tagtcaatat tgaatctaaa tcatcactaa tttctgtaac tccattatga
      901 gtaatatcta taatttcttt agtcatagga agttctccta aaagctctaa gtctaaatca
      961 tctaaaaact tttcagtaga ttctccttca aataacttga tttttttaga acaatcagga
     1021 cattggatat aactcatatt ttcaataact ccaaggacat ttatgttcat ttttttagcc
     1081 atattaacag cttttgatac aatcatagat accaaatctt gaggtactga aaccataact
     1141 attccactta taggaataga ttgcataact gttaaggcaa catctccagt accaggaggc
     1201 atatctatta ataaatagtc taattcttcc caaagtacat cagtataaaa ttgctttaca
     1261 acccctccaa gtaaaggtcc tctccaaaca acaggttcat tttcatcatc tatcataagg
     1321 tttatagaca ttacttttat gttatttgag ttttctacag ggtaaatata agagccatca
     1381 gaataagctt ttacattttt tacacccata agtctaggaa tacttggtcc agttatatct
     1441 gagtctaata taccaacctt atatcccatt ttgtttagct tattagcaag taaagcagtt
     1501 acagttgatt ttccaactcc acccttccca ctcataacac caattatttt tccaaattta
     1561 ttgttagggt tattttcaat agaacaattt gattgactat tacaatttcc ctttgatgga
     1621 catgaattac aatctgccat atctattacc tccaaacttt aatatttatt agattatttg
     1681 taacctacat atactatttt agtacaattg aaccataaag tcattattta aaagcaactt
     1741 agaagatttt ctattgattt attcatagac tatagcaaaa agaatacaat aaaaaaatta
     1801 taacattctt gaaaaagtga ttatttcaat ttagttatag aacttagtca tataaatata
     1861 tagaaaatat tttaatatat tatacataac gggttatttt ataagtttaa ctataaatta
     1921 ggtttatatt ttataaaaat ttgctaaaaa aaacaattaa aaattgtaaa gaaaaaagat
     1981 ataattagaa ctgtagtaat aaatgtttta ttatagataa tagagtttta agtatcttaa
     2041 ataatatttg gaggaatatg aatggaatta agaccaagaa gtgcaaaatt agaagaattt
     2101 gataaagtaa tagaattaat aaactatgta tttagaatat caagaaatca taaaccaact
     2161 atgatggaag aatttcctct tttactaagt aaaaataata tagaaaatat gattataata
     2221 tcagaagatg ataaagtggt ttctgatgta aactatctaa ttcaggatgt atctattcaa
     2281 ggaaacagat tgaaggttgc agctataggt ggagtatgta ctcatccaga ttatgaaaag
     2341 agaggctatt catctaagat attggataaa gtagaagaaa aaatgtttta tgatggtgta
     2401 gatattgtaa ttatatcagg aacaagaagt ctatacagta gaagaaattg tagtttggta
     2461 aagagttttt ataaatatac aataaaacct gaagatgtaa aaatagcata tgaaatagtt
     2521 gaatttgatg aaactaattt tgaaaaagat aatgatttag ataaaatgat tgaactatat
     2581 aatcaaaata gtactagatt tataagaacg agagatgagt ttcaaaaact attgcatgca
     2641 gcaactattg cctggggacc aattggttac aagaaggtat ttataaagga aaataataat
     2701 ataataggat atttgataat aagaacaata aaaaaagaag attctactgt aggagaagtt
     2761 gctgaaatag ggttaaactc tgtaaatgtt gaaaatatat tgaaatatgt tgcaaataaa
     2821 tttggattag aatctttaaa ttataaagta catgtgaaaa atcttaaaga ccagttgaaa
     2881 tgtaatggga caaaatcttt ggattatcaa caaggaacta tgaaaataat aaattttact
     2941 aagttgtgtg atagtttaag aagttatttt agtcaatatg tagattttga attactaaaa
     3001 tacatggaat ttaaacaagt tgaaaataaa tatattatta aatacaaaga agaagaactt
     3061 gtaatagaaa atttggataa gcttaataaa ctgttttttg aaaaaaatga ggaacagtac
     3121 aatgaattta aacatctaaa aaatatatat gaatttgcta caaaggcatt tcctgtagat
     3181 tttccatgga ctgcaaattt aaattatcaa tagttttaga tgatattaaa taaaattgat
     3241 tgatacctag agttagtcct tgttaaataa gagaaattat tggatattga aaaagcaatt
     3301 ttgtaagtaa ttagaaagga tgagtattat atggacatac ttgatgatat agcagaatgt
     3361 atattaaata tgggtgtaga taatatagag aatttagtaa aaattgctat agataaaaat
     3421 attgatgtgg aagatattta tgaatatggt ttaaacaaag gtatgatagg agctttagat
     3481 aagtttgaga ataaggaata ctatctttca gaagttatag tatgcacaga tgcattaaat
     3541 aaaggtatta atcttttgaa gggaactgga aaagtaaaga aaaaatcaaa gggagtaatt
     3601 ctgatgtctg tagtagaagg agatactcat gagataggaa aaaatattgt aaaagtaatg
     3661 gtagaagcca ctggatataa ggttattgac ttaggtgtaa atagaaaaag tgaagatata
     3721 atagaagaag ctataaaaaa caatgtagat ataataggct tatcttcaat gatgacaact
     3781 acaatggaaa atatgaaatc ggttatagac gaacttaata tgattgagat agataaaaga
     3841 ccaaaagtaa taattggtgg aggacctgta agtatggagt ttgcagaaga aataggagca
     3901 gatggataca gttcaaatgc tccaaaagca gttaaattaa taaataaact tataggaggt
     3961 gaagtttaaa tgttttcaga tatgacttta cttcagtata taaatagtga agatagaggt
     4021 ttttttctgc ctgatatggg tacaaatgga ttgttcttaa taaataaaaa ggcatatgaa
     4081 gtatatgata gtgtagattt gcaattagaa ttagcaaaga ctatggacaa gtattttgaa
     4141 tcagatttta tatactcatt ctgtgatggg attacatttt gtgaaactct gggtttagat
     4201 acattaagac cagattatga ttttccaagt gtattgacac atactataac tgacagagat
     4261 aaattaagaa aattagatgt tccagaccct tatacgagtg gacgtatgcc actcaatata
     4321 gagagcttat cagttatatc aaaaagtatt aaaaaaccac tatatgaatc gatacaaggt
     4381 ccatttacac ttgcaggtca attagctgga gctactcaac ttttaagatg tataataact
     4441 gataaaaaat ttgtagaaga attattggaa tttacaactg aattggtaag aagatatgct
     4501 gtagctgcaa ataaagcagg agcaaaatat atttcaatag cagagccaac atcagtaacc
     4561 ctaagtaaag atagatttga tgaatttata gtaaaaaatt taaataaaat ctacaatgaa
     4621 cttgattgtt ggaaaggtat gcatatatgt ggagacacca gagaattatt agataatatg
     4681 ctaagttgta atatagatgc agtaagttta gaccaaattt tagactatga agaaattgct
     4741 ccaatgattc ctagagatat agttttgatt ggtaatttag acccaataaa acttcttgga
     4801 cgttctaagc cagataagat tagaagagag acactaaaat tgttaaagaa gatgagaaga
     4861 tatgataact ttttatgtga ttttggctgt aactgcttaa ataccactcc agtagaaaat
     4921 ttacaagcag ctataaaggc aggaagaatt agttataaag aattagatag gattgatatt
     4981 gatgaattat aatagtaata aaaataaata tcttttaaag gatataaatt tagatgatat
     5041 ttttatagat aagaatattg tgctaaagtt tttggggtat ggaaatagaa aagctccaga
     5101 tacaattagt aaaatcgttg aaagagaaat aagaaatata aatgatattt tagatatcaa
     5161 gatatatata agtgaagttg atattaataa aacacctaaa gaatgtaaaa aagcatttgc
     5221 tgtactatat acaatagggg ataaaattga ttttaagatg aatgactata tggaaaattg
     5281 caatatgatg gcagggctgg cattggataa ggtaggtata gtatgtttag attgtattaa
     5341 tgagaaaata aaaatgtatt taaaaggaaa gtatgatagt ctcaaaatat cgcatgaaat
     5401 atatccagga gataaagatt ttgaagtaga gagacaaaag gatatttata actatgtaaa
     5461 aaataaatat aatagtatag aaatagaaat taatgattat catcaactat cccccataaa
     5521 aagtgttgca atgcttattt taatggggga tgaagaaaat ttagaatcaa gatgtagtaa
     5581 gtgtcctaga aaatgctttt agttaatttt caagactaag atttgttttg tgtataaatt
     5641 gcaataattt ttttggtcat tttatgtata aactaaaaaa attattgcaa agtaatattc
     5701 tatttaattt gaacagactt acaaactata gtagtttctt taattcaata agtgtattta
     5761 tttcataagt tggtatatga ttagtataat ttatactgtt attggaattt aaccaacaag
     5821 tatctattcc tgaatttatt ccacctaaga tatcagaggt taagctatcc ccaattatta
     5881 aggcagaaga tttatcatga caattaaact ttttgagagc gtatttaaat atttcaggat
     5941 ttggttttga tattttcaac tcttcagata caaccattcc atcaatatat tcttttattt
     6001 ttgaattttc aagtcttttc ttttgaactg cagaaattcc atttgttatt ataacaatag
     6061 tatacttttt tttaagatat tgtaatatat cgattgcacc tggaattaaa aatgtacatt
     6121 cacctaaaaa atttaagtac attttgctaa gagtttcaga atcaacatcc aaagatattt
     6181 tatttgcaaa aagttcaaat ctaagtaatt taagttcatt caaagttata atattttttt
     6241 caaggtcgag ccaaagctta tcacttatat ttctatatga ctctatataa ttctcaaaat
     6301 taaattctaa atcaaactca gatagaagtt ttttaaaagc atgtgattca cttttcttaa
     6361 aatcaaataa agtatcatct gcatcaaaaa atataaattt atatgtcttc atggttttta
     6421 ctcctttaca agatgactgc aaaaattact ttactttcac tatttgagtc attttgccat
     6481 ttatgttttg ttcctaaagg aacttttaca ctatctcctt tggacaaagt aaattcatca
     6541 tcatccataa aaagtttaac ttctccctcc aaaacatatg caatttcatc tccattatga
     6601 gtaatcctat caacacaaga agaagtgttt ggagctaaat ccataattgc aaattctaaa
     6661 ttaccactag agtctggaat taaaatctca tatataactt ctttgcttcc aggtaaaata
     6721 actttttttc tattatcatg tcttactatc aagttttttt tatctacctc agatacaaaa
     6781 aatgtaaata aaggaacgtt tagagaagag gctatagatt ttaatgaatt tacagatgga
     6841 ttagcaagac ctcgttctat ttggctcaat aatgatggtg taacatcagc tagttttgct
     6901 aaatctcgaa tacttaagtt ttgttttttt cgaacttcag ctattttttc ccctaaattt
     6961 aaatcattca tataaatatc ctctcttttt aatttaaaaa atattaactt aaaataatca
     7021 agtaaaatga ttatgtagta tgaaattatt ataacatata gaattaggtt ttgaaaaaac
     7081 tatgttcaat ataattaaat aaattaaaat ataattgaca aagtggatac agaatactta
     7141 aaatatattt aaataaaatt aagcaaaaga tagttaattt atgataggag gggatattat
     7201 gcaaataacg acttttggag ctatatttgg tttattaata gcaattattt taattataaa
     7261 aaaatttcaa gctgtatata gtttaatgct aggagcattt attggaggat tagttggagg
     7321 agcaaatata acacaaactg tagattttat ggcaaatgga gctatgaata tatcaccatc
     7381 aattcttaga gctttggcat caggtgtatt agctggaagt ttaataaaaa caggggcagt
     7441 agataaaata tcagaacaga ttgtaaaaat atttggtgaa aagagggctt tattttcgat
     7501 tgccatttca actatggtac ttgctggagt aggcgtaaat ttggatgtat ctataataac
     7561 tgtagcacct ataggattat atataggtag aaaattaaat tattctaaat tgtcaatact
     7621 attagctatg ttgggagggg gtaaagcagg taatataata tcaccaaatc caaatactat
     7681 agcagtagca gataattttt ctgtcaatct ctctagtgtt atgatggcaa atataattcc
     7741 agcaattatt ggagttgtta ttacagttat tttagcaagt atattaataa ataaaggaaa
     7801 taaagtacaa tcatatgaaa ttttagagca aagagaagat ttaccaagtt tatttaaatc
     7861 attatgtggt ccaattatag ctatatttct attgttttta ggaaatgtat ctcctatagt
     7921 tatagaccca atgatagccc ttccaatagg aggaatcgta actctaattg tcacaggaaa
     7981 cttgaacaat tcaagagaat atttagcttt tggattatct aagatgcaag gggtatgcat
     8041 attattactt ggaacaggga ctatcgcagg aattatacag atgtctgaat tacaacagag
     8101 tacaataggt gctttacaat ttttaaatat gccacaattt ttattggcac cagtttcggg
     8161 tatacttatg tcattagcaa cagcatcatc aacagctggt gctactatag catcatctac
     8221 ttttcatgat gctataataa atggtgggct atcaccgatt tcaggtgcct caatagtaaa
     8281 tgctgggtct agtgtattcg agcaattacc acatggctca ctatttcata ctagtgcagg
     8341 tagtataaac atggatatag gtgaaagatt taagttaatt ccatatgaag cattgatagg
     8401 aatagtaatg actataattt caacatctat tcaattagtt ttataataaa tataaatatt
     8461 taatgaaaaa agtaggattt tgttattaaa tattgaattt attatagtaa aaaagaagat
     8521 tggagtttta aagtgaataa aaaaaagatt gttattatag ggattattta ttcattttta
     8581 gtagtatttt cacttacaaa tatgtatgta aatatggagt ataatctaaa tgtatttgaa
     8641 tatattaaga agtcacttcc atttacagaa gaagaaaaaa agtggttaga aaaacacaag
     8701 aatttgattt acagctctga ccaaagttca cctcctttga gatataaagg taaggaagat
     8761 ggacagtata agggaattat tgttgattta ataaattctc tgtcaattca aattggtaga
     8821 gatttttatt ttaaaccaaa taattggtgg aaagaatctt tcgtaaattc aatagatgat
     8881 agtataaagt tttttgactt gattccatca aaagaaaggg caaataaatt tatatttact
     8941 gacccaatat acactttgag tgcaaatata ttgaaagata aaaagtccca agatataaat
     9001 agttacatgg atttaaaggg gaaaacagta gcaattccag aaggtgatta ttctataaat
     9061 tttttaaagc aaaaaattca agatataaac attttgttga ctccagatat aaagactgga
     9121 gtcaaccatt taatgtctgg aaaagtagac gcagttgttg gtgatgaacc agtattgcga
     9181 tactatataa ataattatgg actttcaaat aaatacagtg ttttaagtaa tcctatatat
     9241 actaaaaaag cagttcttgc tgtacctaaa caatatgaag agctggttag tattttaaat
     9301 aaaggcattt ttaaacttca gaaaaatgga gtgtataaag atttaaagaa aaaatggtat
     9361 tctacgtata atgaagttga tgatatattg tatgagcgag gaattgtgcc tagtatttat
     9421 ctttttatag gtataatttt aatatcaatt tatgtttttt atagctatac atatttgtta
     9481 aagatagaaa tcaaaaggaa aacagaacag gtaatagaaa ataaaaaaac tttagaggca
     9541 acttttaata gtattacaga tataataatg cttgtagatg aaaataacaa tatagtagaa
     9601 tcgaacaaag tactatatga ctttatgggt gaaatgagtt ataagatagc agatttgatt
     9661 agcatgataa aaggtgttat tgaaaatacc ttctctgaaa atactaataa aactagtgaa
     9721 atagaaatac acaataaaat tttaaagata aacacttttc ctgtagagta taaaaaaaat
     9781 aatactgagt atatagtagt tcttattaaa gatataacta atgataaaat agtagaggcg
     9841 aaattactta gagaaaacaa aatgatatct ataggacaat tagcttctgg ggtagcacat
     9901 gagataagaa atcctttggg tataataaga aataattgct atctcttaaa agataacgtt
     9961 acaatggaag aagtaaatga ttgtgttaaa tcaatagaaa gtaatgtaga tagggcaagt
    10021 aatataataa ccaacttgtt aaatttcgca agaatatcag atgataattt agaacatata
    10081 aatataagaa attttattga aaatatagtg aaacttcaat ataaaatgtt acagttaaag
    10141 aatgttgaaa taaaaattga ctgtgaacat aatttagtat gctatataaa tggagaatcg
    10201 ttaaaacatg tatttataaa tttaatttct aattcaatag atgcaataca tcaagatgga
    10261 aaaattatta tatactgtta tgaaaaaaat tattgtttat ttatagattt taaagataat
    10321 ggtgaaggaa taaaagaaga tgccctaaag gacatattta atcccttcta tacaacaaaa
    10381 ccaattggtg agggaactgg tttaggttta tacattactt ataatgagat taaaaagaat
    10441 aatggagata taagtgttga aagtaaattg ggggttggaa cttgttttca tataaaaatt
    10501 ccattaaata aagaggtgac tatataatga aaatactatt agttgatgat gagctagaat
    10561 atggagtggt aatgaaaaaa attctacaaa agaagggcta tcttgtagat atagcactaa
    10621 gtggtgaaga agctataaat ataattaaaa aagataaaaa ttatgatttg gttttgtctg
    10681 atgttatgat gaaaaatatg gatggagtac agctattaga tagaattaaa tctattaata
    10741 gagatataga ggttatatta gttacaggat atggaagtat tgaaaatgca gttgatgcta
    10801 tgaaaagagg agctttatct tattttataa aaagtaatcc cattgaaaat ttattagaag
    10861 aagttgaaaa ggtaaaggca agtaaaatat ctgtttctgt acaaaaaaat aatctggaat
    10921 ttactttgga atctaaaaat agagatttta ataatatcat aaaaatagca aaaaaagctg
    10981 cttgtaaaga tgttaatata ttaatcttgg gggaatctgg tgtaggaaaa gatatattag
    11041 ctagatatat acatagcata agtccaagaa agaatgagat atttgtacct gtaaattgtt
    11101 gttctttttc agaaaatcta ttggagtctg agttgtttgg tcatgaaaaa ggttctttta
    11161 caggagcagt agatagtaga aaaggtagat ttgaactttc gaataaagga actctatttt
    11221 tggatgaaat aggagatata cctctaaatg ttcaggtaaa acttttgagg acacttgagg
    11281 acaaatctat agagaggata ggaagtaata aatctattaa agttgacttt aggttaattt
    11341 gtgctatgaa taaagaacca aaagtggaaa tatcaaatgg aaatataaga gaagattttt
    11401 tttatagaat aagtacaata acaataacta taccaccgct aagaaagaga agagaagact
    11461 taactactct tatagaattt ttcttaaata agtaccaaat tgaacatgat aaaaagatac
    11521 actcgattga taaggaagta aaggattttt tattaaatta caattatcca ggaaatataa
    11581 gagaattaaa aaatataata aatagacttg tagttttaag tgaagaaggc aatttatcaa
    11641 aagacaattt aaatttaata tcaaataatg tatacataga tgataaaata agtattagac
    11701 ctttaagaga aataagaaaa gagtttgaat gtgagtatat tgaaaaagtt ttatctttgt
    11761 gtggaaataa tatttcaaat acagctaaaa aattggagat tagtagaaga cagttaacca
    11821 ataaaatatc agagtataat attaagtgag aaaaaaactt ctcattaagt gagaaaaaaa
    11881 tttctcaaac tgtattaaaa gataaacaga aaaaatctgt ttatcttttt ttattataat
    11941 tttaaaaatt aatatttaca ataattattg ctaaattcaa tattggtgta aattaaaaat
    12001 gaaaatttct atgacattaa tacatagaat atatatttta aataattggc atagaaaatg
    12061 ctttatatat taataaacgt aataaatact tttaattgcg attaaatata ttaaggggga
    12121 ttttcatatg aagctagagc tagggaatat atttataaaa aatgttgaat ttggagaaaa
    12181 gacagaagta aaagatggag tactatatat tagtagcgaa gaaatagaaa agatagtatt
    12241 agaagatgaa aggattgtaa gtgtaaatgt tgaacttgca agacctggcg aatcaataag
    12301 aatagctcca gttaaagatg taatagagcc tagagttaaa attggagatg aaagcaaaat
    12361 atttcctgga attataaata aagtgaagac tgtaggaagt ggaagaaccc atgtactttt
    12421 aggagcttgt gttgtaactt gtggaaatat agttggattt caagaaggcg taattgatat
    12481 gagtggtcct actgctaaat atacaccttt ttcaaaaaca aataatatat gtattgttat
    12541 aaaagcaaag gatggtatag atactcatga ttatgaagaa gcagctagaa ttgcaggatt
    12601 aaaaataggt gcatatgtag gtgaggcagg aagagaagtt gagccagatg aagtcattgt
    12661 atatgagaca aagccattat taaaacaagt aaaagaatat ccagacctgc caacagtagc
    12721 atatgttcac atgttacaat cacaaggact tttacatgac acttattact atggggtgga
    12781 tgctaaacag atagtgccta catttatgta tccaactgaa ataatggatg gagctataat
    12841 atctggaaac tgtgtagcac catgtgataa ggttacaaca ttccatcatt taaataatcc
    12901 agttatacat gacttatata aaagacatgg aaaagattta aactttatag gagttatttt
    12961 gactaatgag aacgtattct tagtcgataa agagagaagc tctgatatgg tagctaaatt
    13021 aatagaattt ttaggagttg atggagttct agtgacagaa gaaggttatg ggaatccaga
    13081 tactgaccta atgatgaatt gtagaaaatg tagtgaagtt ggagcaaatg tagttttgat
    13141 aacggatgaa tttcctggaa aagatggcaa gagtcaatct atagcagacg ctactaagga
    13201 agcagatgca gttgtttctt gtggtcaagg aaatctagtg gttcatttcc cagcaatgga
    13261 aaaaattata ggaactttag attatgttga aatgatgata ggtgggtata aaggatgctt
    13321 aaatgaagac ggaagcatgg atgcagagct acaaattata atagcatcaa ctatagcaaa
    13381 tggctataat catttgacag caagatatta ttaaataagt atttgaatat aaagttctgt
    13441 tgatataatc attggtctaa ttgatgtaac aactataaag tgatttggag gattaagaga
    13501 tgagtaaaat cagagtggtt cattatataa acaatttctt tgctggtata ggtggagagg
    13561 aaaaagcaga tattccgcca gaaaaaaggg ctggtgtagt aggacctgga atagcatttc
    13621 agagtcaatt taatgatgat gcagagataa tctcaactgt aatttgcgga gatacgtatt
    13681 ttggtgaaaa tattgagagt gcaactcaag aacttttaaa aatgataaaa gaagaaagcc
    13741 ctgatttatt tattgcaggc ccagcattta atgcaggtag atatggagta gcttgtggga
    13801 ctatttgtaa ggcagttgaa gaagagttaa atattcctgt aattacaggc atgtataaag
    13861 aaaatccagg tgtagatatg tttaaattag atttgcatat agtgtctaca ggaaattcag
    13921 cagcaagtct tagaaaggta gttccaataa tgacaaaact aggtcttaaa ttggttagag
    13981 gtgaggaaat aggacctcct gaagaggaag gatatataat gagaggaata agaaaaaatt
    14041 tcttccatga acttagaggg tctgaaagag caattgatat gttggttaaa aagatgaaag
    14101 gggaaagctt tgaaactgag tatccaatgc ctgaatttga tagagttaca cctatgaaac
    14161 ctgttaaaga tttatctaag ataaaacttg ccctagtaac ttctggaggg atagttccaa
    14221 ttgataatcc agacaaaatt gaatcttcaa gtgctactaa atatggaatt tacgatttaa
    14281 cagatatgga ttctatgtca aataaagaat ttacaactat acatgggggg tatgacagag
    14341 catatgtcct tgaaaatcca aatttagttg taccactaga tgtagttaga gagcttgaaa
    14401 aagaaggtgt aattggagaa ttagtagatt attttataac tactacagga acaggaacga
    14461 gtgttggcaa ttctaagaga tttggagaag agttttctaa aaaattgtta gaagatgatg
    14521 tagatgctgt aattttaact tctacatgag gtacttgtac tcgttgtgga gcaacgatgg
    14581 taaaagagat tgagagagct ggtatagcag ttgttcatat atgtacagta gtaccaatat
    14641 cacttacaat aggagctaat agaattgttc cagcagtagg gataccatat ccacttggaa
    14701 atcctatgtt aggagaagag gaaagtaaaa aaattagaaa gaatatagtt ttaaaagctt
    14761 taaatgcact aacagaagat atagaagaac aaacagtatt tgaataataa aaatacaaca
    14821 caacactgaa ataaaaaaag atatcaaaaa ataatttttt aaaattattt tacgatatct
    14881 tttttttatt aggtaaattc gaaatttgca ttttttaaaa ttaaatttat aaaagtgata
    14941 aattagttta ttataatatt gatacacaaa ttcaaattag atatactatg aaaagcgaga
    15001 ttattacatt ttggaacaaa tattcaacat attagatcat attaattgaa cagaaaaaat
    15061 taactttata taataattag aataatgata agaaagaagg tgtatatatt gatttcaatt
    15121 gccatttgtg atgatgaatc ctatatgcta aatcatttaa aggaaaatat agaattatac
    15181 atgaagcaga agaatttatt atataatata gagcttttta ataaagtaga agatttacta
    15241 tgcaatcatg aaaagtttaa tattgtattt ttagatatac agatggaaga gattaacgga
    15301 atgcaggcag ctagaaaatt aaggtcatat ggtaatgagt gctttataat tttcgttaca
    15361 gttttaaatg aatttgtatt tgatgctttt gaagtagatg ctataaatta tctgataaaa
    15421 cctctatcta atgagaaact atataaaact ttggataaaa tagttacaaa acttgatgat
    15481 aatgaaagta attatatcac aatacaaaaa ggttattcat attggaaatt aaaacttgct
    15541 gatattttat attgtgaagt tataaaaaga aagatttatg ttcatttaaa gaaagagact
    15601 gtagattatt atgagactat tgaaaattta gagagaagtt taccaacaaa tttttttagg
    15661 tgtcatcgta gttatataat caatttacaa tatgtatatg gatatgaaaa tggtaatgca
    15721 gttatggaaa gtggagatag aatacctgtt tctagactac gtcaacaaat attttcaaaa
    15781 gttatgcttg agttcatgaa aaataggagg atataagatg attaatttat tttcttccga
    15841 tttattttta aagtacagtt ttttattttc ttttattgta tatagaattt taatggtaat
    15901 agtgcaatac tattttctaa tcagttttac aaatgttaat tcaaaatttt ggtgttttat
    15961 tttatattcg ataatttcaa tttttttaat cattataaat acatatttta atttatataa
    16021 tttattttct tcacagattc tattttttct tatgactttt ttatttgtaa agtacataat
    16081 aaaacaatct actgttttat ctatagtatc aactttgtta gtatatttaa ttgaacaatt
    16141 agtatatgga tttacagctc cattaaatta cttgatattt actagtgata taaatacaga
    16201 tatttataaa tttgatataa atatgggact atcttcttta attactatta taatagctgg
    16261 atttatatat tggtatagtt ctaaaaagtt taatattaag gtcatgaact ttgataaata
    16321 tatagcaatt ttgatgattc cattgttgct tataatatta tttatgcaat catttgaata
    16381 tagttcaaat ataaatatag atacaagtct tgggattgta aagctgctat taaatacttc
    16441 tataactgaa gaaattcaag cttttctttt ttctataatt ggaactatat gtgtattttt
    16501 ttctctattt acctttaaga aactaattca agctctcgaa gatgacaagg agagggcaat
    16561 tatgaatcaa cagattcatg ctcaaaaaaa ctatattgaa gaagcgaaat cacgattatc
    16621 acaaactata tcctttagac atgattttaa taatcattta gctattgtca atggattgtt
    16681 gaaaaaagac caaattttaa aagcacaaga ttatttaaat aagcttgaaa aagtaacaag
    16741 tgacctatca ttttcttgta atacacagaa tattgttatt gatgctcttt tcaacaataa
    16801 attaagtata gcaaaacaat taggcataga ggtagattgt gaagtaatta taccccaaag
    16861 cactaacatg aacgattttg acctatgtat agtgtttgct aatgctattg acaatgcaat
    16921 taaagcttgt agagttgtag ataaacaaaa taggtattta aagctttcca ctaagttaga
    16981 gggtggattt tttatgatag aaataaaaaa tagctataat ccaataaata attatacaag
    17041 tggctcaggt ataggtcttt taaatataaa agaaattgct aaaaaatatc aaggggctat
    17101 aagtatagat aagacatcag attattttca aataaatgta ttgctggtta tttcattaca
    17161 ttgcgctagt atttgataac aatttcattg aaaatagtat ttgtaagcta tattatgtac
    17221 ttgaatagag aaagtttgat atatagaaat gaggaggtta ctaaaatgaa agttggaaat
    17281 ataaatggtc aagcaccaat tacaaatggt ttagatagtt gtcaaagtga ggtacaaggc
    17341 ttagaacaaa agaaaaaatc attacaggat gaaatagaga aagtaagaag tgataaaagt
    17401 attgatggtg aaacagccaa attaaaaata gaagctttaa aaaaagaaat ggaagagatt
    17461 gaaaataaaa ttcaagagtt aaaaggaaat aaagcaaaaa aaacaaataa agaagaaaat
    17521 aatgtttcag aagtaaataa taatctatta gagagaaaaa aagaaccttc taataatgaa
    17581 aatgtaaaac aattatatga tgtttttgaa agaagcaatg tagacgaaca atgggataat
    17641 acttatcgtg ttctccaaga tgataaaaac ttaaaagtaa aatttaatag acctatatag
    17701 tttatttata aaaatagtta atattttaat tatcaccact ttttagaaga atacacactt
    17761 tattttaaag acccacatat ttatttacag ttagcacatt tatatgtggt tttataacta
    17821 ttatttacaa gtctatattg ataactttta catagtatat aatgtttaaa tacttattca
    17881 aatttatcta tacataaaca aaaaaagaga ttttaaaatc tctttttaga ttgttgactt
    17941 atggcttttt gatttaataa aatcaagaag ccgtttgaat tttatctgtt ggctgatacc
    18001 cttctgatat tgaaacacca tttttgtttc caaaatcgtt taaaattaag aacttattat
    18061 gaatgactat ttcattatga acaataagca gataacatga atcttcaatc aatgttttaa
    18121 cagtagcatt aaatgcttgc ttatacagtt ctatcgtttg tttgaactgt ccattaaagt
    18181 aaatatgagt caataacata actatatcta cacaaattaa tataaatttt ttatactgta
    18241 cattatctaa ctgttgatta tctgtattac aacatgattg aatttaaaaa gccatcaaca
    18301 catagaatgt tgatggcctg aaaatagatt taaaatctct tttttattat atattaatta
    18361 ttttttaaat ttatattttt tgcatcagat tttctaatag ctaagtcaaa ccctctaaat
    18421 ctaatcacta tagggtcatt aagaggagct atctttttca cttcaacttc agtgccatct
    18481 attagtccta aggctagaag tctcttcata agtttttcat ttccagctat attgtctata
    18541 attccctttt gtccaagttt taagttgtaa acagtcatta ttaacacctc ctaatgaaaa
    18601 tcattatcaa actttatatt tataatatat aacaaaaaga gcaaattgtc aatatataac
    18661 aaaaaataaa catactaagt tagtatgttt ataaaaaata gtatacatgg gatactaata
    18721 gcatctagca aaaaaccact tactaatggt acaactaaaa aggcttttgg agaatcacca
    18781 tatttttgtg tcaaagaact catatttgcc agagcgttcg gagtagctcc tattccatgt
    18841 ccaatcaaac cacttattat gatggcagaa tcaaaatctt ttccaagtac tctaaagcaa
    18901 ataaatattg aaaataggat tataaagatt acttgaaaag ttactattaa gagaatagga
    18961 ggtaataatg tagataattc aaataaatct atattcataa gtgataaagt taaaaaaatt
    19021 cccaatgaaa gttcttttaa aaaatctata agtttaaaat ttaattctag tatatttatc
    19081 ttatcattta aatttctaaa aataacagcc gaaaacatac aagttacaac agagggtaat
    19141 agtatattaa acgttatata gaagcatcta cttataattt ctcctgtatt tatacaaata
    19201 aatatgagaa gaacctgttc caaaaataca gatggtgtta ttgtttcgga gaagttatta
    19261 ttttttatcc tattcttatt tctaaaaaat ttacttccaa atctctttag atttctatta
    19321 tatctaggaa tcgacaagtc tatatttgat gatggtttta atttatattt atttattaga
    19381 aatttagcta caggacatcc caatatacca cccataataa gtcctattgt tgcagcagat
    19441 aatccaaccc caactgctcc ttctataccg agattttcta ttgtaagtcc aaatgcagca
    19501 gaataaccat gaccaccttc catagataca tttccacaca taagccctaa aagaggttca
    19561 agttttataa tctttgatat tgtaatagct aatatatttt gacaaaatgc caatactcca
    19621 cacaaaatcc aatatctaaa taatacacta ccacctttct ttataagatt tatgcttatt
    19681 tcaagtccta tagttgtaaa aaagaaggtt atgaaatttg gcattaaatt tccacttata
    19741 gatatatctg ctatattaaa atatttaaaa aataaattta gaaaacaaaa taatagacct
    19801 cctattactg gtgcaggtat acagaaatta tttaaaaagt taactttatt cttcaataaa
    19861 ttaccaatta aaaagaagaa tatagatagt gtcaaggttt gcactatatc aaaacttaag
    19921 gtaatcaaaa taagtcctcc tttgtaattc aaagttttaa ttatataact taaattctta
    19981 tgtattgaaa ttttattatc aaatgtatat taataaatgt atattaaaaa gaatcagtag
    20041 atataattag atagtcataa attaaattaa aatattttaa tgagatatga cttttttaga
    20101 attaaaaacc gtagtatatt gatataatag aaataagact taaaaatctg ttgatagtat
    20161 tatttataag tacagtagga ggataaaatg tttgaaaatt tacctaagat agatttacat
    20221 tgccacttag atggaagtgt tagagtagaa accatgcttg atatagctat taaagagaaa
    20281 atagatttac cttcaaataa tatggatgaa ataaaaaaat tagcaaaggt ttcttttaat
    20341 tgtacttcac tagatgaata tttagaaaaa tttgatttac ccttaaaagt aatgcaatct
    20401 aaggaaaatt taaaaagaat tacttttgaa cttttggaag atgcaagtag agaaaatgta
    20461 aaatatattg aaattagatt tgcaccatta cttcacaccc aaaaaggtat gagtgttaaa
    20521 aatataattg agggtataat tgaaggaatt agagaagctg aaagtattta tgatataaaa
    20581 ggcaatttaa tattaggttg tatgagaaca atgacctcaa aagaggcatt attagtaatt
    20641 gaagaaggga aatcatttgt aaataaaggt gttgtagcag tagacttgtg tggtccagag
    20701 aaagagggat tttgtaagga atataaagat gtttttaaat tagcaagaga atatggatat
    20761 aaagttacaa tacatgcagg agaagctgca agtggagaaa atgtattgga tgctataaat
    20821 attttaaagg cggataggat tggtcatggg gtaaaaatta aagaccataa aaaggcgtat
    20881 aatttagtta aagataaaaa aatattgctt gaattatgtc caactagtaa tgtacaaact
    20941 aagacagtag attcttatga agcacatcca ttttatactt tttataaaga taatttacat
    21001 gtaagtataa atactgataa tagaactgta tcagatataa atttaagttc agaactaaat
    21061 gttatatttg atacttttaa actaggatta gaagactata aaataattta tagaaatgca
    21121 gtagaagcta gttttgcaga taaagaaact aaagagtatt tgaatagttt aatttaatag
    21181 ataaaaatat ttaaacttta aattaagcta ttaaattgat aaaaatacat tgtattagga
    21241 gaaaaaatgt gaaagattat gaagatgttg tttatattaa actagtcaaa aaagaacttg
    21301 aagaatggaa gaaaaatata ttaaagaaac cttctatatt tgacaaagca tcaaagggta
    21361 tacagaataa atttaatgga gtattgccag ataaatatca tgaaataatt acttcttcta
    21421 taaaagtaat gacaaaaacc gtactgtttg gatataaata tgctacaaag ccaccactta
    21481 gaaatatgtc attagaagaa agagaaaaat tagttttaga aaaagctaaa acttacaaga
    21541 ctacagcaat gattgaaggg gcaggaacag gggcaggagg aatcctctta ggtttatctg
    21601 attttccttt gcttttaggt attaaagtga aatttttata tgatgtagct tcaatatatg
    21661 gatatgatgt taaagattat agagaaagga tttatatatt aaatatatta cagttggctt
    21721 tttctagtca acagcatata aacaaaactt ttaaattgat gaataactgg gatgaatatg
    21781 taaatagtct accagaagac atcaatgatt ttgattggag aacatttcaa caagagtata
    21841 gggattattt agatttagct aagtttttac aactaatgcc tggaatagga gcatttgtag
    21901 gattttatgt gaatggaaaa ttaattgata aattatgtga aactgctgtg tctgcttata
    21961 gattgaggat tcgagagctt aagttataag aaaaattata acataaaaaa ttagttatac
    22021 aatatatcta tatgtatcta attttgcatt gataaaaaca attatggata aaaggtaaag
    22081 catgatagaa tctaatgtag aggtttgtat tttaatggaa aaatttagtt tttattgctt
    22141 aagatacaaa ttgaattacg aagagattga attatgaaga gaagtaattg ggaggaatat
    22201 tttcatgaca aatttgccaa aacaatttga tagcttcaat gaagctagac aaaaaggatt
    22261 tataaatgca aaggaactta aggaaagtgg gaaaaagatg gttggagttt tctgtacatt
    22321 tactccagtt gaaattccaa tggcagctgg agcaactgta gtaggtgtat gcggcgttag
    22381 tgaagagcca atacctgatg cagaaagggt gcttccaaga aatctttgtc ctttaattaa
    22441 atcaagctat ggacatgcaa taacagatac ctgtccttat ttctattttt ctgatttgtt
    22501 gataggagaa accacttgtg atggtaagaa aaaaatgtat gaagaacttg caaaagttaa
    22561 gccaacatat gtaatgcatt tgccaaatac ttcaaaagga gaatttgcct ataaactttg
    22621 gaaagatgaa atgataagat taaaagaaga ggttgagaaa tctttaggtg taactataac
    22681 tgaagaagat ataagaactg ctataaaaga taaaaatgaa gaaagagaac ttttaaaaga
    22741 gttttatgca cttggtaaat tacaaccatc tgcacttaca ggccttgagt tacataatgt
    22801 tttatatcaa gcaggtttta aatttgaccg tgcagaactt aaacattctt taagaaaagt
    22861 tatagatgat atgaaagaaa gatatgaaaa aggtgaatgt cctgttcaaa aagataagcc
    22921 aagaatattg ataacaggtt ctcctattgg tggaataagc gaaaaaatag ttaaaacttt
    22981 agaagatgca ggggcatcag tcgttgcata tgaactttgt ggagctatta gatcaaacga
    23041 tttattggtt gatgaagaaa tagaagatgt atatgatgct ttaacacaaa aatatataaa
    23101 tataggttgt tcttgtatga tgaataatga caatcgtatt gaattattag atagaataat
    23161 agatgaatat aatgtagatg ctgtaataga tgtagtttta caagcatgtc atacatttaa
    23221 tatagaaagt tatagaataa gagaatttgt tacaaaagaa aaaaataagc catttatgtc
    23281 tcttgaaact gattattcaa aatcagatac tgaacaatta agaactagat ttgaagcgtt
    23341 tgtagagatg ttgtaggaat aaataaaagt aggaggaact atgtttagta taggagttga
    23401 ctcagggtca gtggcaacaa agggtgtgct atttgatggt gaaaaaatta ttaagaaaat
    23461 aattatacca actggatgga gccctaaaag tacatctaaa caggtctatg agttgctaag
    23521 tagtgaaatt gacaaaaaag atataaaaaa ggtggtaggt acaggttatg gaaggggagt
    23581 aatggatttt gctgataaga aagtaacaga aataacttgt catactagag gtatttactt
    23641 tttaaacaag aacataagga ctattttaga tgtaggtggt caagatagta aagttataaa
    23701 tttagataga gatggaaatg tatttaactt tataatgaat gataagtgtg ctgcaggaac
    23761 tggtcgattt ttagagatta cttctaacct tttaggaagc gatattgaaa gtatagatac
    23821 attagctaaa ggatatgaac cagttaatat atctagtatg tgtacagttt ttgctgagtc
    23881 tgaaattgta agtcttctag ctcaaaacat ttctactgga gaggtagcag cagggatact
    23941 taaatctatt gcaaataaat ccacctcaat gttagcaaga ggtgaggtaa tagatgaagt
    24001 tgcatttact ggagggcttg caaaaagtaa agaactagta aaaatgatag aagaaatact
    24061 gggtaaaaaa atttttattg cagaagatac tcaaataata ggagctttag gagctgctgt
    24121 aataggattt agataaaaac atataaaaag aaaatgagaa gctgaattta ttataaataa
    24181 attcagtttt ttattttatt ttatttattt ataaaggaaa actttggtat aattgattta
    24241 aatgattgta tatttgtaaa tacttgatat ttagtcatat ataacattat aattttaaaa
    24301 attagctcaa aataaggggg agaaagcgtg aaaaaattta cttcaaaaaa agtaacaagg
    24361 tttgttgcgc tgtttttaat atctatttta gtaatgtcaa caattcctgt atcagctgac
    24421 aatagcaata cattgaatca gataaaaaaa gcagaataca gtgagacgta taaacaatat
    24481 ctaaatgatg ctaaaaatgg acgtacagaa aaatataatg ggattatacc aaatccatat
    24541 cagttggaag gtactcaaat ccaaagtaaa ggaagaacag ctccaacatc ttatgaccct
    24601 agaaaattag gtcttatgac atcaataaaa aatcaagagg atttaggaat ctgttgggat
    24661 tttgctgcaa tggcaacctt agaaagtttc ttaaagttaa ataattatgg agactatgat
    24721 ttatcagaag aacacttaag atggtgggct tcagatggtg aatatggatg gtctgtaaat
    24781 gatatgaatg gtgctttaaa ttatgaagct atgggatatt taacatcttg gtcaggtcct
    24841 aaattagaaa aagatatccc atacaatggt agagtatcta aagcacaagg agcaaaaaaa
    24901 ccaactaata tgaatacagc acctactgtt tttaatgtga cagatgctgt gtgtgtaagc
    24961 aatgacataa attctacaaa aaatgctata ttacagtatg gagctgtaac atctggttat
    25021 tatgaagata ttaaatatca aagtgatgac caaaattcat attattgtcc aacaaaaagt
    25081 tttaatacaa atcatgctat ttcaattgta ggttgggatg ataactattc taaagataat
    25141 tttaatgcaa atattagacc atcaaaaaat ggtgcttggt taataaaaaa tagctgggga
    25201 gattataatt ctgagggtgg atatttctgg atatcttatg aagacaagac acttatgtct
    25261 gatatagata atttttcaat caaaggtggt aaaaaaccaa atgatgatga gaagatgtat
    25321 caacatgact atgcaagtat tgtaccttta atttcaaaaa agataacagc tgcaaatgta
    25381 tttgatttca atagaggcga tgaaactcta aaatctgtaa tattcttaac tgaaagtata
    25441 ggagctaagt atgaagtata ttatgctcca gtagtaaatg gtatacctca agaaaacaat
    25501 atgaaaaaat taaaagaagg tactgcccaa tattcaggat acataacagt tccaattgat
    25561 tcttttgata taccagaagg aaagggtgca attgtagtca gtatagaggg gaaaaatgga
    25621 gagtcaacta taggttctga gtcaaatgta ccaggatatg atacatttaa agcaaaagct
    25681 aatttaggtg aaagttatat catagataat actggaaaat tctttgatat aaatagagat
    25741 agtaacttct atccgtgtaa ttttactata aaagctgtta cagaaaagtc ttctggtgag
    25801 agtatcccaa atgagtcttt aattggtagt gatagatatg aaactgctat caaagttagt
    25861 caaaatggtt tcaattcttc agaaaatgta gttttagtaa atggcagttc aatagtagat
    25921 gcattggcag caactccttt tacatctgct ataaattcac caatattatt gacacaaaaa
    25981 gaagctttaa attcaaaaac taaagctgag atacagagat taggtgctaa gaaagtatac
    26041 ctaataggtg gagaaaattc tataagtaaa gaaattgaac aacaattaaa aagcttaaat
    26101 atatcgatag agagaatatc aggaagtgat agatacaaga caagtttatt attagctcaa
    26161 aagttaaatg gtataaaaaa tgtatctcaa atagcagttg taaatggagt gaaaggatta
    26221 gctgatgcaa ttagtgtagg cgctgcagca gcagaaaata acataccaat catacttgca
    26281 aatgaaaaga gcgaattgca aggagctgat gaatttctaa attcattaaa tatagagaaa
    26341 tcttatatta ttggaggaac agcatcccta tcaaacaatc tagaaagtaa gttaaaaaat
    26401 ccaactagat tatctggtag tagtagagac gaaactaatt caaagataat agataatttt
    26461 tataaaaaag atactttaaa aaatgcattt gtagttaaaa atggaatcaa gaatcaaaat
    26521 gatttaatag atggattggc agttggtcct ttaggagcaa aaactgggtc accagtaatt
    26581 ttagttggtg ataaattagc tgatagccaa aaagaagttc ttaaaaataa gactttagaa
    26641 aaagtaactc aagttggtgg aggagcaaat aaaaatgcat ttaaagagtt agtaaaatta
    26701 aaaagtgcta aatagagtac ttaagtatta acatatatta gtgtgtatta gtaacttatc
    26761 ttaataaaag ctgaaaataa ttacttcaaa ttattgataa ataagataat caatgattgt
    26821 aaagttttgt tttcagcttt aaatttatgt agtataagtt taaaaacata aaaaagggtt
    26881 actttatata gaaatataga aaatcttaca ttttatatat aaataaccca tatttaatta
    26941 actttttata gtgttctaat agatattatt gaacttaatg gtaaatataa tgtgtaagtt
    27001 tcatttaaag ttggattaga ttcatctata tatcttgagc aaagtgtaat tgataagcta
    27061 tctgcttcta taatttcacc atatagagga caactgctaa agtaagaatg tacttcaaca
    27121 tatttattta agttttcttg aacataatcg taaaaacggt tttttgtagt agtagccata
    27181 aaatcaactc ctttattttt tattaaagtt gaaactttta aaaagataaa tatgattact
    27241 tctattagaa actaagttaa aaaataagca aatagatatg aaaattagaa taatatatta
    27301 tttatatctt aattataaca ctagataata aaaaatgcta tataaaattc agaattttcg
    27361 aaagttttta agcttatttt taaatatact aataatacta ttgacattag atagaaaaag
    27421 tattatatat gactatataa attattttag tcaaactgga gggaaaagat gcctaaatca
    27481 tattctgata aagaaagaga gtatataata aaaagactta aagaagaagc aagattgtgt
    27541 atggagcaat atggtattag aaaaactact gttgatgagc ttgtaaaacg ggtaaaaata
    27601 ccaaaaggaa ctttttatct attttttcaa tcaaaagaat tgctcttttt tgaggtatta
    27661 agagatatac atgactctat acaaaaagaa atcttatatg agataaatag agtagatgaa
    27721 agtattacat gtgaacaatt gacagatata tttatgaaat tctataggat ggttgatagt
    27781 acttcaattt taaatcttat gataaatgga gaatttgaga tattggtgag aaagttacca
    27841 gattctataa ttgaagaaca ctttagacat gatgactttg aaattggaga gattatatct
    27901 tgcataccaa atgcaaaaaa taaagacata gaaagcttta gtggagcatt tagagcagtt
    27961 tttcttacca tgctatataa gcgcgaggtt ggaagtaatt gttttgaaga tgctttaaga
    28021 ttaatgataa acggattagt tatacaactt atggaataga atttaaattt tgtttgctaa
    28081 taacttttat taaaaattat taacaaacaa aaaaattata ttttattgac catatagtca
    28141 aaatagtcaa aaatttgaaa gggtgatatt gaatgattaa agttgatgat ttatcattta
    28201 gttatacaga gagagatttt ttacaaaata ttaactttgg agtaggaaaa ggggaaatac
    28261 taggattttt aggaccatca ggtgcaggaa aatctacatt acaaaagatt ctaattggaa
    28321 tgataactaa ttatggagga agtgtcattg taaatggagt agagagtaaa agacactcaa
    28381 ataaatttta tgaaaatata ggagttgatt ttgaatttcc tagtttatat gaaaagttga
    28441 ctgcaataga aaatttaaaa tattttgggt ctctatattc taaaaaacta ctatctattg
    28501 atgagttatt aaaatcagta ggactagaaa atgaagcaaa taaaagggta tctgagtatt
    28561 caaaaggtat gaaatctcga ttaaatttta taaaagcttt attacataat ccagatattt
    28621 tatttttaga tgaaccaaca agtggtcttg acccatcaaa tagcaaggtt atgaaagata
    28681 taattttatc agaaaaatct aaaggtaaaa ctattatttt aacaactcat aacatgttag
    28741 atgcaacaga gctttgtgac agagtagcgt tcattgtaaa tggtaaaata tctgctttag
    28801 atacaccaca taatcttatt atgtcaaaag gagctattaa agttagatat acttactttg
    28861 ataatggaga aaaaacttct gaatgttttc tgaacaatac tgctaatgat aaaaatttga
    28921 atatgcttat tgagaaaaat aaacttttat caatacatag tagtgaacca acattaaatg
    28981 atatttttat tgagataaca gggaggaatt tacaatgagg ctaaaaaatc ttattttagg
    29041 agatataaag tttcaattta agtatggttt ttatttttta tatctttttt taagtattat
    29101 atatatttgt attatcaatg tatttccaac ttttatgaga gaaaaaatag ctataattat
    29161 gatttattct gacccagcag caatgggatt gttttttatg ggagctattg tacttcttga
    29221 gaaaagtcaa agggttttaa attccttagc tgtatctcca gtaaaagtat ctgagtatat
    29281 tttatcaaag gtaatttcat taggagtaat atctagtatt gtagctatgt ttattgctat
    29341 tacattaaat ttagataata ttataatatc aactataggt accttttttt cttctattat
    29401 attttctttg cttggattga tacttgcaag taaagcatct agtttgaatc agtttattgt
    29461 tttaagtatt cctattgaga ttatatgctt tattccacca atactcaatg tacttcttga
    29521 cacaaaatca tatgcaaatt tgtacccttt taatatatgt atttctctta ttagtggaga
    29581 taaaaatttt ataatgatta atattttaat tttaatatca attataataa taatttattt
    29641 tataacttat tattttattt gtagcagttg gaaaaaggta ggtggagtaa aactatgata
    29701 aagattataa atgcgtttaa acaagagttt gaacaaatta agagagatgc tatgcttttt
    29761 atagtatgtg tttctccaat attatgtgga gtatttataa aatttggaat accattaata
    29821 cagaatatat cacttaacaa gttttattat caattaaatt tagagccgta tttcttgatg
    29881 tttgatttac ttttagcttt tattacacct tttatgttct tttttgcttc tacaatggtc
    29941 atattgggag aaattgatga cagcatttca agatatttaa tagtaactcc gcttggtaaa
    30001 actggttatc ttatttcacg atttggaata ccaggtatct tagcatttat tattactatg
    30061 attctactta tctttttttc tctaactaaa atttctttct tgttaaactt atcaatttct
    30121 ttattagttt tattacaagg aattattata tcaatattag tgatttcttt atcatcaaat
    30181 aaactagaag gaatggtaat tacgaaattc tcaggtgttt ttatgatggg tattttagca
    30241 ccatttttta ttttaaataa agcacaatat attctgttct tcttaccaac tttttggata
    30301 tcgaaggcat ttaaggaaga taattatgtc tatatgttta tttcaattgt tatatcatta
    30361 atatggattt tattattatt taaaaagttt agtaaaaaaa tagttaaata agaaaattta
    30421 atatataaat gtaaggttga cttgacagta atgttgaaaa atgttacatt taagatgtaa
    30481 agttaacctt acatgaatgg agcaaatatg aaaaataaaa taaagatatt aagagaaaaa
    30541 ttaggattga cacaagaaca actagggaga ttagttggaa catctagaca ggctataaat
    30601 gctatagaaa ctggtaaaaa tgaaccttca atatggttgg catatgatat ttcaagaatt
    30661 tttaatgaac caattgaaag tgtattttta tttgaagaaa gtgaaagaaa atcaagagca
    30721 cagataagta ggggagaata ttatggctat aagaaagata agaacatttg atgatgaaat
    30781 tttaagaaaa aagagtaagt atgtagaaaa cgtagataat aagataagag aaatattaaa
    30841 tgacatggca gagaccatgt acaatactcc aaatggagga ggattagctg catgccaagt
    30901 aggagtatta aagcgattag ttgttataga tttaggagaa ggacttatta agcttgttaa
    30961 tcctgaaatt attgaacaag aaggggaaca aattgttgtt gaaggatgtt taagttttcc
    31021 tgaagtatgg ggtaaattaa aaagaccaaa aaaagttaca gtgcaagctc taaacgaata
    31081 cggtgagaaa atagaaatta aaggttcagg ttttatggca aaatgtcttt gtcatgagat
    31141 agaccattta aatggtatag tttttacaga taaaattatt gaacatgtaa aattgtaaga
    31201 tagattggtt attgaattta taaaatattt tatagtaagg agttaaatta tgagattatg
    31261 gcataaggat ttaattgatg tattacctaa aaatcaatta gtaagtcaat ggagagagct
    31321 acttgctata aaaggttcta tagataaaaa aggtactcca aaccatctat tagtgaataa
    31381 agtattgaat tatagtatag atgaatttaa attttataca aaaattgtac atgatgaaat
    31441 gttaaaaaga aattataaac ctaatgaact taaatataca agtatattaa aatggaaaaa
    31501 tagaaatttt gcaaatgata tatctaatga acatagttta aatttagaaa atttatatga
    31561 tgattggcat aataaaatgt atttaaagca atgcttatat aatttagaag aaaaagcaac
    31621 atgtggagga ataccaataa atgaatggaa tatattactt tgtaaatata gtaaagatta
    31681 tgaattgtgg tctggaaata tcatgtttta attactatag attaacatta tagtattatt
    31741 gtatataaag aggtgattat ttgtataata aaataaaata tattccaata aattgtgaaa
    31801 tagcttgtaa taaattaaaa aggagtatgc catataaatg ggatttaaat atttatcgtg
    31861 gatgtgagca tggatgtaaa tattgttatg ctatttactc tcataagtac atcaactcca
    31921 ataattattt tgaagagatt tatgtaaaaa caaatatagt agaaaaactt gagagacaac
    31981 ttaaaagcag taaatggaag aaagaagtaa tcaatatagg aggagttaca gatagttatc
    32041 aaccaataga agctgactat aaaattatgc cagaaatatt aaacctgcta ataaaataca
    32101 agacaccagc tattatttct acaaagtcag atttaatttt aagagattac gatttaattg
    32161 ataagctatc taggattaca tatatcaatg tagcatctac aattaccaca gtaaatgaag
    32221 atacacaaag gcttattgag ccaaatggtg tggattcaat gagacgattt aaaatgttaa
    32281 aagaatttag aaaaactaac gcatcagttg gtctacatat tatgccaatt ataccatata
    32341 ttacagatga ttttgataat ataaattcgc tatttagaca tgcaaaagaa tgtaatgtac
    32401 attatgtttt acctgggact ttatacttaa ggggtataac tagaggtgtt ttttttgact
    32461 ttgttaaaaa agagtttcct gaattatttg ataaattatc aatactatat agtgcaggtt
    32521 cacttaatag ggagtataaa aatcaattgt ataaaatggt taatgaactt agatataagt
    32581 attctctatc aagcagttac tctaaaataa tgaaggaaaa gttaaaaaac tcaggagatg
    32641 tacagatatc tttttttgat tagttttata taaaaaaatt ttttttacta tgatgattaa
    32701 aatttattaa aattaaaatt tattgacaaa tataaaattt aaatatataa ttaattcata
    32761 aaataaaaat atgaaaggat gatgttgttc ggtgatattt gatatcataa aatcgttgat
    32821 ataagtattg ctggaaaaga gtgaataaaa tcaaatttaa taagcaaaca aagtaaagct
    32881 atagttttat tgttgtgctt aaaaatattt ggttctattt tactcttatt taaactcatc
    32941 tttttgaagt taaaccacag agtaaactct gtggtttttt tattttaaaa ataaatttta
    33001 aggagtggta tgttttgtta aacaaattat gtatgtattt agaaagacca gagctttata
    33061 aacaaagtga aattaatttt tgggatgatg agtatatttc aaagcaatta ttaaaagcac
    33121 acttagacac taattttgaa ggagcaagta gaaattttaa ctttatcgag gattctgtaa
    33181 attggatagt tacagttgca aatccagcta attatcctaa attacttgat ttaggttgtg
    33241 gtccaggttt atatgcagag aaatttgcac aaaaaggtta taaggtaact ggaattgatt
    33301 tctcaaaacg ttctattaac tatgcacaaa atagaaataa ggagactaat ttaaacataa
    33361 attacttgtt ccaaagttat ttaaatatga actataatga ggaatttgat ttggctactt
    33421 taatttattg tgattatgga gctttgtcaa ctgaaaatag aaggttatta atggaaaaaa
    33481 tttatgatag tttaaaacct ggaggaaaat taatattaga tgtatttaca ataaataaat
    33541 acaataattt tgaggaaatt aaatattggg aaataaatga agatggagga ttttggagta
    33601 atgaaaaata tatgtgtcta caagataatt gtaaatacaa tgattataat acattagaac
    33661 aaactcttgt aataaccgaa aaagatgaaa atgtatttta tgtttggaat cactattttt
    33721 caaaagaaag tttcttatta gaagttgaaa atattggatt taagtctgtt gagtttttta
    33781 gtaatgttaa gggtgaagca tattctgacg atagtatgac tatgggatta gttttacaaa
    33841 aataataatt tttttaaaat gaataaataa aaacttgaaa cctttaacaa taaaataaca
    33901 taataaaagc ttacatattg aaaaatggaa acttttatgt tatttttagt acattacaaa
    33961 tttgcttata taaaaatcaa tacttgaatt attattttta tgtgaaattt atctatagta
    34021 tatttttata aacaagtaat gttattaaaa ttaagtatgt agttaaaagt gtatagcaaa
    34081 aaggctgtta tattaatgtt agaggttcag ggaaaatata taaagcaaag tatttacaga
    34141 taatgtagat tgcacagcta taagttagat aatagaatta tgtaatcaag aatttacaac
    34201 aaagtataag ataaggataa tgctaaattg ccatacaggt gctagatgtg taaatggatt
    34261 taattaatac tattgataaa gtgcaacaag cacataaacc aattgatgag attattaaaa
    34321 ctataagaga tatagttgac ataattaaaa tattggaact tgtttataat tttaaatcaa
    34381 atcaataatg attaagatta aaataatatt aaggcatcaa tgttataaga gtacaatata
    34441 aggagtacta tatagtactc cttacttata catattaggc aataggaaat tttttcatag
    34501 ttaaattttc aagtattttt taggaaactt tataattgag aattaagggt atagtatgtg
    34561 atgaacttac tgaatttata attaggttat ataatggaat aatcatcaat agttttactt
    34621 tagggaaaat atatgcttaa ggaagtatag cttactaata ttattaaaat gtatatttaa
    34681 aatgataaaa aaagaattag acaagtattt agtaaattga ctatgcaaaa ttctatcaag
    34741 caggaaccct atgactttag tcatgggagg ttcagtaggt aacttgttga attaagttta
    34801 tattatttaa ataaactact taatttagat aatataaaaa gtcagaattt taagaataga
    34861 gctatataat cttagctatt aggagaatta ctatgatagg aatatatttt agtggaacag
    34921 gaaattcaag atactgtgta gaaaaatttt tacaagaata tgatataaca gcaaattctt
    34981 attctattga agataatgaa cttttacagc acatcaataa tcatgagaat attattttta
    35041 gttatccggt gcaatatagt aatgtgccaa aaatattgaa agattttata ataaatcatt
    35101 caactttgtg gaatggtaaa cgtgttttta ttattgcaac tatggctcta tttagtggag
    35161 atggctctgg tgtgttatct agaatattaa aaaaatacgg tgctataact gttggtggat
    35221 tacatgtgaa gatgccagat agtataggag atgaaaaggt attaaaacat tctttagagt
    35281 ataatagaaa gcttgtaata aaagcagaaa agaaagtaac taaagctgct aaaaaactga
    35341 aacatgggtc accaccaaaa gaagggttag gttttttgag tcatattgta gggttatttg
    35401 gacaacgatt gtattttatc aacaaaacaa atatgtatac agataaacta aaaattaata
    35461 ctgaggaatg tattgtatgt ggtaaatgtg ttaatttatg tccaatgaag aatctagtaa
    35521 ttaaaaattg tatgattgtt gcaaatgggc aatgtacaat gtgttatcgt tgtgtaaata
    35581 attgtgctaa aaaagcaatt acattgttag gtaaaaaagt tgttgaacag agtaatatta
    35641 aaaaatatat gtaagaaatt taaagtttta ttgaatgtaa attgatgtat attttatgtt
    35701 agaaagagag gttaaataat ggaatactac atagttgatt catttgcaac taaactattt
    35761 aaaggaaatc ctgctggagt ttgtgttctt gacagaagaa tacctttaga gttaatgcag
    35821 aaaatagcag aagaaaacaa tttaccagaa acagcatttg tagttaaagg taaaggaaat
    35881 tatgaattac gatggtttac tcctaaagca gaaattgatt tatgtggtca tgcaacttta
    35941 gcagcagctt atgtaatatc aaattttatt gatgttaatg taaaaaggat agatttcttt
    36001 actcaaagtg gaaagcttga agttacacga aatggaaatc tatatgaaat gatatttcca
    36061 gaaataatgc ctattgaaat agagttatca ccacaacaag ctaatttgat aggctgtgta
    36121 ccaagtgctg tttattcttc aagagattta atcttattgc taaacagtga acaagaagtt
    36181 ataaattata aacctaatta cgcacaattg cgtaaattga cagactggtt aggtataata
    36241 atcacagcac aaggaagtaa tacagatttt gtatcaagat atttttgtcc agaattagat
    36301 tcagaagacc ctgttactgg ttcatcacat tgtaatctta ttccatactg gtctgaaaag
    36361 ttgggaaaac acaaaatggt agctgctcaa ctttctaatc gtggtggtat aattcagtgt
    36421 gaagtgttga aggataatac tgtgaaaatt tctggtgaag cagtcttgtt tatgcaggga
    36481 acaataaaga ttgatattta aataagtata ataactaatt tattatgaaa aataattaat
    36541 tttcttagtg ttaacaataa taaagtagag ttaaaaaact tgatttcact taaatcaata
    36601 tgatagatgt tgatactaag attgacaagc atgattaaaa aagataatat ttaatctata
    36661 aagataaaaa cacagtctgg ttggtagtcc agacgcagca atagctgtca gtaacctgcc
    36721 tccttggttg tccgttcttt agaaagtaat ttaaggagga actagcaatg gacaaaatta
    36781 gtggaaaatt aacagtctta tttgaagaac cattttgggt aggtatattt gaaagacaag
    36841 atggtaaaaa atatgaagca tgtagggtag tttttggagc agaacctaaa gaagtagaag
    36901 tgtatgaatt tatacttgaa agatttttct cattagattt tggtagcatt aaattagaaa
    36961 aaaatgtgac caaagacaac attggataca aaagaatgca gagaaaagta aaaaaggaac
    37021 aagaaaaaga aactataggg actaaggctc aaaatgctct taagcttcaa tatgaagaaa
    37081 gaaaacagga tagaaaaaac ctagctaaaa atagaaaaga agaagaaaaa gaaagactat
    37141 ttaatttgaa acaagaaaag agaaaagcta aacataaggg tcattaattt taattaaata
    37201 tctttttaat aattgctaga aaaaggtgta taaaattttt aattttatac accttaattt
    37261 attgaaaaga ataaggtttt atatttcaat tactttaaaa tataaaaata tactaataaa
    37321 aattgtaaaa atagtataaa tggtatacca aatgtaaatt taatgtgttt agttttatgt
    37381 ctaaaagaat acattccaat tatagaacct atactacctc caattactgc tatagacata
    37441 agagtagctt cttttattct ccattcgttt ctaattgctc ttttcttatc tatgtacatt
    37501 gaaaagaaag caatagaatt aatcactata aaatatatta gaataaaatt tttcacagtc
    37561 ttgcctccat aaaaatatat tatacattat aaaacaagat atttgttttg gaaagttaaa
    37621 tttgtaatta gtacatataa aatatttgat aagaacaaat ataatattaa ttggaaaatt
    37681 taaaaaaatt aaacaattag caaatagggg gtatatttat aagacacaaa gtgataaaat
    37741 ctatttagaa aataatgtaa aataacagga ggataaagca aaatgaaatt tatagtagaa
    37801 aaagaagttt ttgataaatt agaaaatgta tgctttggtg tagtagttgc aaaaggaata
    37861 gataatacta aagaaataga aagaataagc aaccttttag atataagtat agatagagta
    37921 gaagattact tcaaagataa aaaagtaaag gaatcagaag aaattatacc atatagagag
    37981 gcatttagaa gtctaggcat gaacccaaat aaatttatga gttcgataga agcaatgaca
    38041 acaagggttg ctaagaataa gaaacttcct catataaatc ctattgtaga tttaggaaac
    38101 tctatttctt taaaatattt acttccaatg ggtgctcatg atatggattt tagaaatgat
    38161 gatgtatatg ttagattttc taaaaaaggg gataaatttg taccatttgg agaaactgat
    38221 gttgaattaa tggaagaagg agaattaatt tattcagttg gagacatggt aaaaactaga
    38281 agatggatat ggagacaagg agaagaagga aaaattacta atagctcaaa aaatatattt
    38341 tttcctatag atggttttac tgatgctaat ttagataagg taatgagtgc aagagaagaa
    38401 ttagcaaaat tgttaaagga aatatttaat tgtgaaatta aagtaggttt tgtagataaa
    38461 gataatcctg aaatggaaat ataataagtt gttgaatact tgttaagata ttatatttac
    38521 ttaatgatgt taaaaaggta ttaaaaaaga aaggtcgtag taaatttgaa aaaaactata
    38581 ataaagaatg taaatccaat gattgaaaat aagtttatag gactttttga aatagagtat
    38641 aaaaacaaat tagatgaaga taaggtttgg atggtagcat caagaaaaag tagtgagcaa
    38701 ttaaaaaata tttatttgaa gaatgaagaa gatagtgtag atgcagttgt aatagttggg
    38761 cttcataaaa gtagtaaaaa attaatttta ataagacaat ttagagtacc tattaatgga
    38821 tatatatacg agttaccagc aggtcttgtt gatgaaggag agtctataga tatttccgtt
    38881 gaaagagaac ttagagaaga aacaggactt actcttttag aaattaaaaa aaataaaagt
    38941 attgataaag tttatttatc tcctggaatg agtgatgaat ctgttgcctt tgtttattgt
    39001 atatgtgatg gtgatataac agatgaattt ttagaaccag atgaagagat tgaagcgctt
    39061 ttaatttcgc aagaagatgc aaaagagatt ttacaaagta atcataagat agacacaaaa
    39121 gcatttttga ttttacaaat gtttgtgagt ttaggagcta aattatttga atagatatat
    39181 agaaaagagg ttatatagaa gagtaaaata ataaagttta ttttacttgt gtaaattata
    39241 atctcttttt ttatacaata aagtataatt ttgtgtattt ttaaataagt gatttgactc
    39301 ttaaattttc ataatatgtt ataaaccaat ttaagatact acaataatta aaattccaaa
    39361 attatttaac atatttgcaa ttaaaatgag aaaaggtaaa ggctgaatga ttgaaaaatt
    39421 tgatgtaaaa tagtagctta gtttaatact atagtttgta gggttaaaaa ttagaaattg
    39481 taagggagat aaattctatg aaaaaattag ttattatttt aattagtatc attagtatgg
    39541 ttagcttgac agcttgtaaa aaaagcaatg aaagtaaata taataattta aacaacaaag
    39601 aatccggaca aacaaccatt gaaatggact tagataaaaa ttatgatact tcagacccat
    39661 ttgttaatgc acgtttgttt tgtgtgtcta atgatataga tgttttagac acagagattt
    39721 cttttaaaat ggatggagat agtggtattg tggaaataaa ggacaataaa acagatgaaa
    39781 ctttatggag taatacatgg catggaagcg ttgataatga tacattcaca atttctcttt
    39841 ctaatataca aaaagagaaa gagtatgcta tatgctttac tggaattaaa ataaatcatg
    39901 cagttgtaaa ggttactttt gaaagcaatt tagttaagga gaaagaaaaa ccctcaaaat
    39961 aaattccagc ttttctataa cttgatattc agtacataaa agcagttgat atttatgatt
    40021 gactgctttt tcttatcaaa aaggataggt tgtgcatttt tacttgaaat agtttatgca
    40081 catcaaaagt tggtgagcat gatattggaa atggtaagca tgaaatcttg ataacacaag
    40141 gaaataaatt taagttttca attaagtata tttccat
//
LOCUS       DAEORZ010000021        40034 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEORZ010000021 DAEORZ010000000
VERSION     DAEORZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40034
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.021"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            190..864
                     /locus_tag="KRH39_000972"
     CDS             190..864
                     /locus_tag="KRH39_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8510893.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIDNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            852..2012
                     /locus_tag="KRH39_000973"
     CDS             852..2012
                     /locus_tag="KRH39_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8510894.1"
                     /translation="MDKINNMSLKKTFFVLTLSSLIIASVLTTFSYILLNNLYNSIQD
                     KYLKTPINTGFITIVQNNVNQFSDYDKTLLNIISILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEEKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHDGSISIENNSTNSAKITAKFSTRN"
     gene            2229..2756
                     /locus_tag="KRH39_000974"
     CDS             2229..2756
                     /locus_tag="KRH39_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBG8510895.1"
                     /translation="MKSFANNITFSRIVFALLMVSSTFCSLPFWMCYFWCGISDILDG
                     FIARKLRQQSVIGAKLDSIADFIFAVTILVTVAKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGASIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            3041..3709
                     /locus_tag="KRH39_000975"
     CDS             3041..3709
                     /locus_tag="KRH39_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510896.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSIIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKEYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3712..6180
                     /locus_tag="KRH39_000976"
     CDS             3712..6180
                     /locus_tag="KRH39_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8510897.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITLGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGNLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTASFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTIAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTSISLI
                     FSLISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSILDKIDNM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSTLEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTLDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSDLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFAAIFGSIAGYVSAIIINIATIEKLAFSNIPITSILQATIISILAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6421..7845)
                     /locus_tag="KRH39_000977"
     CDS             complement(6421..7845)
                     /locus_tag="KRH39_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8510898.1"
                     /translation="MKNQSLIAQFRRTFIFIIIASIVATVITYVFAIYLYIYSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIIALFSPFIYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNRISYEICDSGSGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7864..8556)
                     /locus_tag="KRH39_000978"
     CDS             complement(7864..8556)
                     /locus_tag="KRH39_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8510899.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8608..9387)
                     /locus_tag="KRH39_000979"
     CDS             complement(8608..9387)
                     /locus_tag="KRH39_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG8510900.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELLVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            complement(9384..10154)
                     /locus_tag="KRH39_000980"
     CDS             complement(9384..10154)
                     /locus_tag="KRH39_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG8510901.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIFVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTVLEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10151..10855)
                     /locus_tag="KRH39_000981"
     CDS             complement(10151..10855)
                     /locus_tag="KRH39_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG8510902.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDSNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(11091..11603)
                     /locus_tag="KRH39_000982"
     CDS             complement(11091..11603)
                     /locus_tag="KRH39_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510903.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSVYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKIPSDKELKSIVFTIQNEGNAIK
                     LYDTQDETYSGCKIIVGMEFETGCVFVDGSDELNDEITAFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            11929..12186
                     /locus_tag="KRH39_000983"
     CDS             11929..12186
                     /locus_tag="KRH39_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510904.1"
                     /translation="MILNRTELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKGDYEQAEALINSKLR"
     gene            12329..12955
                     /locus_tag="KRH39_000984"
     CDS             12329..12955
                     /locus_tag="KRH39_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBG8510905.1"
                     /translation="MKINIKSTLNEFIKQNNLFDDNRIISYLPNITIDTDKIKTIMIN
                     EVVPTNPNDDFYSIDKDADYLKTTIPLFNNAGIVVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13205..13984)
                     /locus_tag="KRH39_000985"
     CDS             complement(13205..13984)
                     /locus_tag="KRH39_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG8510906.1"
                     /translation="MNSKIYYFSGTGNSLVVSKTLKEMLNESKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KKEKKLSAGFLINMPGNALITPPDVEEERLRNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTDRKKYHHPDITVKDIFLDK"
     gene            complement(14052..14513)
                     /locus_tag="KRH39_000986"
     CDS             complement(14052..14513)
                     /locus_tag="KRH39_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8510907.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNKIANVYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14489..15166)
                     /locus_tag="KRH39_000987"
     CDS             complement(14489..15166)
                     /locus_tag="KRH39_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8510908.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKNIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVFSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEAQGII"
     gene            15400..15786
                     /locus_tag="KRH39_000988"
     CDS             15400..15786
                     /locus_tag="KRH39_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8510909.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15789..17588
                     /locus_tag="KRH39_000989"
     CDS             15789..17588
                     /locus_tag="KRH39_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBG8510910.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLILPFVPLKYINLGNVFNYLYTFNQLGSNTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFTFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNRDILINYIMCFFQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSYIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHIESSALKFKSILIFTLVAL
                     IVFGNIPRLSIIADINEKYRPTNDKTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALFGLENGIITRDNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTKLKNIQTYLKQIGYGNHDVSGGISNFWLESSLKISPIEQVKLLESF
                     YTNKFKFEDKNIEAVKDSLLISKKDGVSLSGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(17941..20028)
                     /locus_tag="KRH39_000990"
     CDS             complement(17941..20028)
                     /locus_tag="KRH39_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBG8510911.1"
                     /translation="MIKVVYMRKNKKTRKMIIFGFLMCLLIVIIVFLAKFILDGLNKN
                     KPDEVFKQYMSFANKKQYEKMYDLLDEKSKSENKKEDFVLRNKKIYEGIDAHDISIKI
                     NDIKKNKNNDKVINYDSKMDTLAGEISFSNEVLLTRDRQKNYKIKWQSNVIFPELEED
                     NTVRVSKLKGKRGRILDRNGVMIAGQGLASMIGLVPGKMSDNIEDLKKLSTLLNVSVE
                     QIEKKLNASWVKENSMVPIKTIEKIKENTDGTVKEEDKELQESLLSIPGVKISNTEVR
                     VYPFGEKTGHLTGYVQNVNADILKEKEGKRYNDNSIIGKIGLENLLEDRIRGIDGYEI
                     IIADRYGDKKETLVTEPKVDGEDVKLTIDSKLQSKLYDQMKNDKGCVVVMNPKTGEVL
                     SLVSSPSYNPNEFILGMSEDRWNELNKNENKPMYNRFKARLCPGSSFKPVTAGIGITT
                     GKINPNENFGHSSLSWQKDSSWGSYKVTTLKDYGNTANMKNALIYSDNIYFAKAALKI
                     GEDVLAKELLKLGFDESMPFEFGLSSSKFGTDNKFETEIQLADTGYGQGKLLVNPVHT
                     ASIYSAFVNDGDMIKPHLEFKKAEFWKKGVFSKEAVKIIRDDLLQVVESPDGTGHSAM
                     IKGISIAGKTGTAEIKASKDDVTGTELGWFNAFTVDENSDKQYLIIAMIEDVKSRGGS
                     HYVIPIVKSVFED"
     gene            20389..20967
                     /locus_tag="KRH39_000991"
     CDS             20389..20967
                     /locus_tag="KRH39_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8510912.1"
                     /translation="MSTKGEQTKQFIIDTSIMLFSEKGYSNVTMKDICEACNLSRGGL
                     YRHFNSPKEIFISILNAHKYYSQNLLDKGIKENISAKHMLEYFFQQQKADINGKYCGF
                     DFAIHEFAFVEKDQRDYMNKRYSSAITMLSNLIDYGQSKSEFKKCDSKSVAIHIMFLF
                     DSMKTSASILKLTNKDIDNQINIIKDMVILYE"
     gene            20960..21619
                     /locus_tag="KRH39_000992"
     CDS             20960..21619
                     /locus_tag="KRH39_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HBG8510913.1"
                     /translation="MNKQKFHKIDLQNWERYTHFYYFSKMTNVGFNLTIEIDITNLLK
                     ETKKRNIKFYPAYLYTITTCLNQYRELKIAYDEDELGYWNTLTPLYAIFHDDNKTFSS
                     IWTEYDENFSIFYENYLADVKEYGKNHGFLAKPNPPRNCYTVSCLPWISFKSFTVHNH
                     GLENYFFPTIEAGKYYQKYEKILLPLSITVHHATTDGYHVSCFIDYIQKMCFNTKIWL
                     H"
     gene            complement(21831..23705)
                     /locus_tag="KRH39_000993"
     CDS             complement(21831..23705)
                     /locus_tag="KRH39_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8510914.1"
                     /translation="MNKYNKIANELIEIIGENNIISITHCATRLRVMVKDREIINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVV
                     FCFIATCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVT
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(23710..25374)
                     /locus_tag="KRH39_000994"
     CDS             complement(23710..25374)
                     /locus_tag="KRH39_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBG8510915.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIVSRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKAVRNQPGMEKMLYELKENTFKKYDCMTVAEAAGISYDQLGEFIGEDGFFSMV
                     FDFKYADLDVASGSEWFKRISWTISDLRKYILDSQMYVQQHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRNTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWSDKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDNVIAYRRQLDLVVIDCY
                     YNFSDKPVEIKTEEKYDIVFQNLDDINLLNKTVSLKPFQAVLLKVR"
     gene            complement(25388..26209)
                     /locus_tag="KRH39_000995"
     CDS             complement(25388..26209)
                     /locus_tag="KRH39_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG8510916.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKLQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIQRLQERLGITQKET
                     ICFGDGQNDIVMFQASDITIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(26308..27072)
                     /locus_tag="KRH39_000996"
     CDS             complement(26308..27072)
                     /locus_tag="KRH39_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8510917.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKKMKMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(27069..27818)
                     /locus_tag="KRH39_000997"
     CDS             complement(27069..27818)
                     /locus_tag="KRH39_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8510918.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYYAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(28100..29152)
                     /locus_tag="KRH39_000998"
     CDS             complement(28100..29152)
                     /locus_tag="KRH39_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8510919.1"
                     /translation="MNVINVFNSNLKRNINKRIVFLVTLLFPIIIVVLGVLANYISKP
                     SFNIGILDEYKNECILKESEKRTYENTIMALENTQGINTEIADFKTIKTDIITGKYSA
                     VICFNKNGFELYSIKDKNTNDTLKYLIETYKKDPVPVDISKLQENTLGVLQRIIAFIV
                     LFLMITSTVTASMIIKDKNSGTFTRVLYSPQNIRGYVLGNMLYNFTITYFQYIVSISI
                     IKLFNIDIGMDYIDLILMGVWISALATSFGTFVSSLFNKEMHANLFSTCISLILSLIG
                     GSFISVEKMPTMLQKISIISPTQWFISFVTYIEERDGSFFNMNYICILTFIIIILALL
                     AMKFTQRSKYSSNRNY"
     gene            complement(29152..29892)
                     /locus_tag="KRH39_000999"
     CDS             complement(29152..29892)
                     /locus_tag="KRH39_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8510920.1"
                     /translation="MRCFLTLFKLGIKRRSKDFFILFYNIVFPVIIIVLLGYLTSKSY
                     GKEFTSYNYYTIVTIPFCVLMGIISVSYVAQDEKISNTSYRYMISPISIVDLVISKLF
                     SCTTVFSIYNLITLVVLRGIFHMSFGGKFLVVIFFLMCESIAVVSMGLFLGLVCKNLD
                     MLRNIINIPIVIFGFLGGVFFPVSSLNPVISLIINISPLTWINRGIVACIYDNNLQLL
                     FNISCIFVAVSIFMTILTIKFFKKEVFI"
     gene            complement(29897..30655)
                     /locus_tag="KRH39_001000"
     CDS             complement(29897..30655)
                     /locus_tag="KRH39_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510921.1"
                     /translation="MSNVLLEIKNLSKKYNNKEVVSRESFKLLEGDILGFIGPNGAGK
                     STSINMLTTIVSPDSGSIYYKGKDIKNQQNIFKSSLGVVPQELAIYEDLSAYDNVKFF
                     CSLYGFKGNELKERTKYALEFVGLWESHKEFPSKFSGGMKRRLNIACAIAHSPQILIM
                     DEPTVGIDPQSRNKILEAIKGINNNGTTVIYTSHYMEEIETLCNRIVLIDKGVIIEDL
                     DKSLYKNKYLKFGCKTLEDIFLYLTGTDLRDLEE"
     gene            complement(30760..31482)
                     /locus_tag="KRH39_001001"
     CDS             complement(30760..31482)
                     /locus_tag="KRH39_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8510922.1"
                     /translation="MIRTLILYYLNIKPTHGYEIQKFLQVSGADRWTKIQSGSIYYAL
                     AKLEKDGGVRVLKEEKTGARIRKIYEITQSGKLELREEIQKELQMPIVPTGSNKFLLH
                     NILDVLPKDTLQKNLEKHIKYLIEQKKYWENWKEIKKIDKKSLATEKIAFDMTIDNLN
                     YQILWHEEILNNIDKYISVGCETQNIIKSIDFSNIEEDFLFTSDTTNELLEVQRLRDE
                     IINNPDKAIENVDKIILKLQNK"
     gene            complement(31583..32188)
                     /locus_tag="KRH39_001002"
     CDS             complement(31583..32188)
                     /locus_tag="KRH39_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8510923.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGENIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKVISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            32367..33005
                     /locus_tag="KRH39_001003"
     CDS             32367..33005
                     /locus_tag="KRH39_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8510924.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            33137..33799
                     /locus_tag="KRH39_001004"
     CDS             33137..33799
                     /locus_tag="KRH39_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8510925.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            33799..34980
                     /locus_tag="KRH39_001005"
     CDS             33799..34980
                     /locus_tag="KRH39_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8510926.1"
                     /translation="MKTPRNINITVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(35161..37821)
                     /locus_tag="KRH39_001006"
     CDS             complement(35161..37821)
                     /locus_tag="KRH39_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8510927.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYDMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(37818..38501)
                     /locus_tag="KRH39_001007"
     CDS             complement(37818..38501)
                     /locus_tag="KRH39_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510928.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTYGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(38845..39798)
                     /gene="blaCDD"
                     /locus_tag="KRH39_001008"
     CDS             complement(38845..39798)
                     /gene="blaCDD"
                     /locus_tag="KRH39_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG8510929.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYSIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKCVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEVAINIIKKYYSVRE"
BASE COUNT        14149 a         6444 c         4810 g        14631 t
ORIGIN      
        1 tcaaatatat attctattaa aatttctaat aatttcatcc ttgatttatt taatatataa
       61 aaatatacta actttaatga ttaagttttg aaatataact aagaatttga gaatttattg
      121 agaatttatt gagattttta tactaaagta tacatactat acaaacttaa tttagaaggg
      181 agcattctta tgaacaataa aatactcgtc gtagatgatg aggaaggtat aataacactc
      241 ttaaaagatt actttgaaat gaatggttat gaggtttaca ctgctcaaag tggaaaagac
      301 gcattaaaaa aaatatccaa aaatccagat attattctac ttgatataaa catgcctgaa
      361 atagatggaa ttgaagtttg taatagaatt agagattatg tatcatgccc tattctattt
      421 cttacagcta gaattgaaaa tactgacaag ataaatggtt ttcgtgctgg tgctgatgat
      481 tatattgtaa aaccttttga tttagacgaa cttggagcta gagtatctgc ccatttaaga
      541 cgtgaacaaa ggaagtcaaa tcaaactaaa attaaatttt ataatgattt aacgatagat
      601 tacgaaaaaa ggttaatttg tatagataat aatcagattt cactttcaaa aaaggaattt
      661 gatatagtag agcttctctc tatgaatcct ggacaagtat ttgataaaga aaaaatatat
      721 gatagagtct ggggatataa tagcaatgga aatagtgatg ttataatgga acacattaga
      781 aaaatacgaa tcaaactatc aaaacatact cttgaaaatt atatagaaac agtctgggga
      841 gttggttata aatggataaa ataaataata tgtcattaaa aaaaactttc tttgttctta
      901 ctttgtcttc actaattata gcatcagtac ttactacttt ctcatacatt ttgcttaata
      961 atctatataa ttctatacaa gataagtatt taaaaacacc tataaataca ggttttataa
     1021 ccatagttca aaacaatgtg aaccaattta gtgattatga taaaacgtta cttaatataa
     1081 tcagcatact tcagcttgta cttccactta tattttttat aggactttta ttattagctg
     1141 atataatttt ttacaaggta aaactaaaaa cacctataga aatactcaat aaaggtgcaa
     1201 tggaaatatc caacaacaat ttagactttt gtttagaata taataataat gatgaattag
     1261 gtaatctatg taatgctttt gaaaaaatga ggtctgaact aaataaaaac aacataaaga
     1321 tgtggactat gattaatgat agaaaacaac ttaatgctgc tttttcccat gatttaagaa
     1381 atcccctaac agttctaaaa ggttactcaa actatttaac aaaatacata ccaacaggta
     1441 agcttagtga tgagaagatt ttatcaacca cacaacttat gtctgagcat attaacagaa
     1501 ttgaatacta tgtagaaaat atgagtaatg ctcaacgatt agaagattta gttgtttcaa
     1561 aatctatgtc gaatataaac aagtttatag aaaacttaga tgaaaatata tctatcattg
     1621 ctaaacaaga agaaaaatct tttacactta aaagtcaaat aaataatata aatctatttt
     1681 ttgatgaaaa tatcattctt agagtcgtcg aaaatataat atcgaatgct tttagatatg
     1741 ctagaaataa tgtatccata cttatatatc ttgaacaaga actccttacc tttgtaattg
     1801 aagatgatgg tataggattt agtaaggaat ctctaaaatt agctttaaga cctttctata
     1861 gagataagac tttaaatgac agcaatgcac actttggtat gggtctttat atctcaaaaa
     1921 tattatgtga aaaacatgat ggctctattt ctattgaaaa taattctact aacagtgcta
     1981 aaattactgc taaattctca acaagaaatt aaaatgattt tgtaagcttt cactgttctt
     2041 atataagctt tgttagttta gaatttataa aatgtatttc aatttacaac caatatatat
     2101 actattttgt agaagttgta agagttgctt caattataat attttggcaa aaggcatttg
     2161 aaataataat agaaatatat atttaaatat gtagaatagt atgaaattag aattttggag
     2221 gtgattatat taaaagtttt gctaataata ttacattctc tcgtattgtg tttgcgttgc
     2281 taatggttag ttctacattc tgttcactgc ctttctggat gtgttatttt tggtgtggaa
     2341 ttagtgatat acttgatggt tttattgccc gaaaattgag acaacagagt gtaataggtg
     2401 caaagttaga tagtattgcc gattttattt ttgcagttac aattttggtt accgtcgcaa
     2461 aaaatattgc tcttcccatg tatctatggt ggtgtattgt tgcaatagca ttgctacggt
     2521 tgattagtta cagtattggt ttttttaagt atcgtacatt ttcatctctt catactttga
     2581 tgaataaaat cactggggct tctatctttg cttttccatt actctatatt tgctttggtc
     2641 ttgatgttgc aggaattatg atatgtatcg tggcttttct ttcttctttt gaagaaatag
     2701 caattatgat aaaatcaaaa gaattaaatc gtgatataaa aagtatattt atgtgacaac
     2761 ttagaatttg tgacaaaaat aatttatata ataatgagga agtaaacata aaaaattatc
     2821 cacaagttta tgtgtaaaat agttttaaag caatagagtt gtattgtaaa acctttagtg
     2881 cataaattac attttaaatt aaaaatgata ctgaaactgc ttatcagcac tgcgaattat
     2941 ccgtaaatag tgaagacttt ttagatttaa actaaaagtt gggaaaaaat tgagttttaa
     3001 aacttacact cttcttatat agaaaaggag agtgtttttt atgattataa aagcaaaaca
     3061 attatcaaaa atatatggtt ctaataacaa taaagttatc gcacttaata atgtaaattt
     3121 agaaataaac tcaggtgaat ttgtttctat aattggacct tctggaagtg gaaaaagcac
     3181 acttttacat attttaagtg gattagataa tcctacctct ggacaagtgt tacttgatga
     3241 taaagatata tataaacaca ctgaaaaaga actatctgca cttagaagaa aatcctttgg
     3301 attcgtcttt caacagttta atcttttacc tgttttgaca gcatctgaaa atatttcaat
     3361 gcctgtactt ctagataaaa aacaacctga taaagaatat ttaaatgaaa tctcatcatt
     3421 acttggaatt gcagatagac tcaatcatct tccccatgaa ctttcaggtg gtcagcaaca
     3481 aagagttgca attgctagag cacttattgc aaaaccagat ataatatttg ctgatgagcc
     3541 tacaggaaac ttggacagta aaagtggcag tgaagttatg aatcttctta taaaaacttc
     3601 aaaacagttt ggaaagactt tagtggtaat tactcatgat gatagaattg caaaacttgc
     3661 tgatagaaaa atttcaatta ttgatggtgt tcttatggag gtgaaataga aatgaagaat
     3721 tacctttcac tttcaataaa agaattaaaa agccagaaac ttatgactac attcatcatt
     3781 atagcaatag tactatcaac tattatgaca actgtggttg gacagtcaat tggtatccta
     3841 caaaatttaa gaattgaaca agctcgttct tttaacggtg atagacatgt atcctttcac
     3901 caacttacta aaaatcaagt tgatgacata aagaaagatg atagagttta tcaagctggt
     3961 acttcaataa ctcttggcag tagtaaaata aaggattctg gtatctctgt acttgtaaaa
     4021 gagtatgata aaacaggact ttccaactac cccaaattaa tgaaattaaa atctggaaat
     4081 ttgcctaaag ataaaaacga aatagcctta gatgaaaaca cactaaagct tatgggtata
     4141 aaaccaagac taggagtgac tatacctatg aatttagata tttcactttt aaatgatacc
     4201 ataccaccat ataattacac agctagcttt aaattgagtg gcattttaga ggatgattat
     4261 acaggatatg taagtggtat agtaaatggt attgtaggta aaggtacatc agaaaatcta
     4321 ttacctaaga gatatatttt atcctctttg gattttaaaa taaaacagca agaaaagttt
     4381 caagaaatag taaatcaatt agctaaaaaa ataaatcttt ctcacaattc aattcaatat
     4441 aattggattt acttaaatgc tcttaagata cagtttgaaa aagatgaaaa tagttctaat
     4501 agtgatggta tatctatgat tatattggta tctttatttg ttgccttatt ggttttactg
     4561 gcttctggac ttgtaatcta taatatttta aaaatctctg ttacaaagaa aattaaggag
     4621 tatggatgtt taagagcaat aggagctgaa ccaaaccaga tatataagat agttatttta
     4681 caaatcttaa ttttatgtac tattgccata cctattggag ctgtaattgg aattatatct
     4741 tctaaaggca taactggaat ggtcactaat atcttaaatc cagatatact tctagcaaat
     4801 gataataaag aaattactga gcttatacat aaaaacacaa cagcatatat gttcccatta
     4861 gtattaagca ctagtatatc tttgattttt tcacttattt cagcattacc atcagcaata
     4921 tatgcttcac atgtctctcc caaaattgct atggctggca gtactaccaa aatcaaaaga
     4981 aaaattaaac gagaaaaaac aataaaaaac tttgaaagac atctagcctg gctaaactta
     5041 aaaagaaata aaggaagaac tataataacc atactatcat tatttatgag tataacagta
     5101 ttcgttgcac tgagtgagtt ctctaatgta ttagatgtat ctaggtcagt aagtaattta
     5161 aaagaaggag atttttcctt aacaaatgaa atatctggtt ttgataagtc aatactagat
     5221 aaaatagata acatgaaaaa cgtcaatcgc acttctttta taaagtactc tgaatacaaa
     5281 cagggcgaaa tagatacaga tataaatttt gaaaatagtg gtgagatgtt aaaaatcatt
     5341 ggaattgatg aacaaacttt aaaagactta atgccttcta ttacagattc tacattagaa
     5401 gactttaaaa atggttctat ctgttttata aaaaatccaa ttgctatttc tacaccaggt
     5461 gtaaaaacta gacatacaaa tttaaaacct aaagatagta tcacaatcaa caaaaaacaa
     5521 ctagatatat atagtacatt agataaaatg ttctttttac aaggtaatgg ctggataaat
     5581 ggtgttgatg ttattgtata tgattctgtt tataacacct tgacaaataa aaacaagttc
     5641 aaccagataa atatttatgc taaagataaa tcagatttag aacaaataag attgtctatt
     5701 gaacagattt gtgaaaataa tcctggctct cattggatat catatattga atcagataag
     5761 caacttaaag agagttttaa acaaatagaa tttctagcat gggcagttat attatttgtg
     5821 ggattgattg gtactttaaa catcattaac acaactcata caaatataaa tactaggact
     5881 aatgagattg gagttaaaag agctattggt atgagtaata gtagcttata taagatgttt
     5941 ttatgggaag gtgtttatta tggaattttt gctgctatat ttggttcaat tgctgggtat
     6001 gtcagtgcta ttattataaa catagcaact atagaaaagc ttgctttttc taatatacca
     6061 attacttcaa ttttacaagc tactattatt tctatacttg catgtattat agctactctt
     6121 attccattaa gaaaagttaa aaaaatgaat ataattgact gtatagatat caatttataa
     6181 ctattttaca gataataata taaattaaat tatcaatgac tgtacccatt ttctttcaaa
     6241 tctaactaat aaacctttcc aactataata taagttttaa aaaaaggcct gtctcaaaat
     6301 agagataagc cttttttcat tgttgaaata atgaaaatat atttaacttc acaaaaaaag
     6361 agtgcctcac gaattaaaaa tttcgctttg agacactccc ctttaattta aaatatatag
     6421 ttaaatgcta tgccaaaatt tcacacatgc tccacctgat tttgaatttt ctattttaac
     6481 agaacctcca agctgttcca ctaattgttt gacaatgtaa agcccaagac cagaatgtcc
     6541 tcctttagtc tgcctagctt catctcctcg ataaaactta tcaagagcct tttttaaatc
     6601 tttagagcta aacccactac cagaatcaca aatctcataa gaaatacgat ttttttcatc
     6661 ctttacagta atactaatat taccacctga tggagtgtat tgcagactgt tggatatgat
     6721 attatcaaaa attcgtgtca acctatccac gtcgatttga attggggatt gtatattccc
     6781 ctgcattttc aatataagtt caacttcctt ttgatgagcc tgtaattcat agtcttgtac
     6841 cttttgcctc aaaaattcag gtaaatttat aggaacaaga tttaattgta tatttgactt
     6901 ttctaagtca gacgtatatt gcatcttttg gacaagagca gcactttttt ctgtattttc
     6961 tcttatcact gtaagatacc tatgaagttt ttcattgtca tctgtattat taccaataag
     7021 tgcatctgta tatcccaaaa taatagaaag aggagatttt aaatcatgtg ctagagcttc
     7081 caccatttca acacgttcct gctccatctt ccactgtgca gataaagagc cttttaattc
     7141 gtctttcatt tcagaaaagg cactacaaag tttaccgagt tcattatctg aataatagtc
     7201 gatttcaaaa tcaaggtcct tttcttttat cttcttggac gcatcaatca atagatgtaa
     7261 tggttgattg atattcttta caaagcgctt agaaagccat ctagtaaaac ccacgatata
     7321 tataaatgga gaaaatagag ctattataat aactgcaaat acaaatctcc ctctaatatt
     7381 tgcaaaggtg attttcactt ggtagaatag aataacagct ccctcaatct ttccattatc
     7441 ccctttgatt ggaacagtat ggatataacc acctttacga acagttttat tgataaaatt
     7501 attaaataat tcttcttttg ttttaaatag ttttttgggg ttagtaccat atagaatatt
     7561 gccattatta tccacaactt gatatagcat atcatcccca cgaatcgttc tttttaaacc
     7621 ttcttcattc gattgtgata acaaagctat gtttttttca ttgatgtatt tttcaattct
     7681 aggaacttgt cgctcataat agtttggtgg atagatatcc ttatttaagg aataaatata
     7741 caaataaata gcaaatacat aagtaatcac tgtggcaact atactggcta ttataataaa
     7801 aataaaagta cgtctaaatt gggcaataag ggattgattt ttcatttaat aacctctctc
     7861 tctttacatt ttattccact tatacccaat accccaaact gtggaaatgt attctttttc
     7921 tggtgtcaca gcagaaaact tagctcgtat tttttttact cgttcaacaa cagttgagga
     7981 atcaccttca gaatcacacc cccatacttt ttcatagatt tgttctcttg agaaaacttg
     8041 tcctgcatgg agtgctagta attcaacaat atcatactct atttttgtta aatcaatatt
     8101 cttaccatca attttaattg cacgctcctt catatataag cataacttcc caaaatacaa
     8161 agtagatctc ttgttctctt cattgatata ctgtgaacgc ttttccctac gcagattagc
     8221 ttcaatcctt gccataagtt cacgcagtcc aaatggcttt gttacataat catcccctcc
     8281 aaaggtcaac cctttgattt tgtcggtttc tgattgcttt gcacttaaaa atataattgg
     8341 gcataaaaca tcatctctaa ttgcctgaca tacctcaaat ccattcattt taggcatcat
     8401 aatatccagt ataataaggt caggctcctg ttttgataac tctatacctg cctgaccatc
     8461 ataagctatc aacacttcat gtccttttgc cttcaattca tcctcaagta gcattactag
     8521 gtccatttca tcatcaataa tcaatatttt actcaatata acaccacctt tctcttatgg
     8581 gaaggtaagt attaaaacaa tattcgtcta ctcataactt ttccttccct cccacatttt
     8641 aaaccaaata acgcctccta tcaagaatat aatcaaacct attgctacta ctagaagctc
     8701 tatactagct gtttgcatca ctttagagta aaatgcctca gttgaaatag ttccagcttc
     8761 tttaataaaa taaattcctg gaagcatacc tagttttact ggccatgtcc atggaacaaa
     8821 tgcccatact ttttcaccaa cgctagttaa tccaagtatt gctgccatca aaattccacc
     8881 gatactaata ccaatagatg cacccatacc ccatgcaaaa ctcacccaaa gatgaatagc
     8941 taatagtgga agtgtcccaa tcaccacaag tcctgctgct gacaggaata accatattgt
     9001 tgctccagat ggcactgcaa tactcattcc tatgcaaagt atcaatgtag caataaatgt
     9061 gcatatagtt aggcaaaaca gaagaaaaag aaattttccc agatataatc taacacgaga
     9121 aatacctaca cataaaaaac cattaaaatt tccagcaagc tcttcctcct gcactataaa
     9181 tcctgctaaa atacctactc caataggaat gatgaataca gtccatactg taaaaaatcc
     9241 ttcaaaagca aattcttgtg tacttccaga tttagttgtg agataaaaaa ccacacataa
     9301 tgaaaaaata ataggcatac aaagagtaag ccactggaca gctgtcctct ttgctcgaag
     9361 ccattctgat gataatagtt ttatcatttg tcatccctcc tattgaacca agctgcagta
     9421 agtgctgtta atactataaa tacaaccaaa gaaacaacaa tcccaatagg aatcactgaa
     9481 gtattcaaaa gtgggtctcc agcttctaat acagtaccgt taggatgtat gttgatgatt
     9541 gggcatacca ttctaactgc ccaactccaa ggtactgcaa tccaaaatgg ttttgtcgca
     9601 gcaaggacac caaatatcat gcctaaagct ccaactccca tacttaggaa catacctttc
     9661 catgtagcta gccatagctg gagtggaagt atagcaagag aaacaaccca gcatacaata
     9721 cttgccccta taatctgtcc aaaaggaact ggaccagctt tcaatattaa accagttata
     9781 attgttacga atattaaaac tagcgttgaa ataaaactgt agactgccat agctataact
     9841 ttattaatcc aaagtgtcat aggagacaca ttgtgtgtac gtaaagcacg ataatttcca
     9901 gctttttttt cctggaaagc taccaaagta gcaaacaatg caaatccaat aggcagaaat
     9961 aaaaatgacc accagttaaa aattaatgct aaaagatttt gccacgccca agaagttgtt
    10021 tgagataatg gattttgcat gagtgttgat tgcattataa atgcatatgc tgcaaaagac
    10081 actgggaaaa aaacaataag tttacccata aatgttcttt tatactttaa aagttctgat
    10141 tgtaaagcat tcatctgttt tgtcctcctt taagaataac atctgtaaaa aattcttcta
    10201 gattttcatt agagtcaggc attccttgaa atagtagtcg tcctccattt ataattccta
    10261 tattatctac aacttgagca acctctgata gaatatggct agaaagaatt acagttatac
    10321 ctttttttgg aaaagaagca atcaattcac gcaattcttg aataccaaaa gggtcaagac
    10381 cattagttgg ttcatctagt attaacaact ctgggtcact caaaagtgca atagctattc
    10441 ctaacctttg tttcataccc atagaaaatt gtgaagctct ctttttacct gtatttttca
    10501 aatttactat ttccaacact tcataaattt tatctttagg tattcccaat atttttgtat
    10561 gtactaacaa attttcctct gctgtaaggt ttccatacaa tgcaggggat tctattaaag
    10621 aacctatttt tgcaagagat tctcgtttcc aaggctttct atcaaaaata atacgtccat
    10681 ctgttgggcg aagaagacca acaagcattt taagtgttgt ggactttcct gctccatttg
    10741 gtcctaaaag tccatatata ctatttcgtt caatttgtag agaaacattg ttaactgcca
    10801 tttgttctcc atatcttttt gataagtttt ttgtttcaag aattaaatct ttcatattaa
    10861 tttctccctt cttgtaatac cttaaatcta ttactaagag aatactaaca tgaaaaaata
    10921 acaattctat aacacctact aattttaata aaaattaaaa taaaaaatag agttaccaat
    10981 tttattttgg caactctatt taagtttatg tttaacagtt atattagatt taagcttagt
    11041 ttcattatca cactaacttc aaatttactc tataattctt aaatcaaaca ctaatctatt
    11101 aatttatatt tctttagaca tctaatatag tttgctacta aataataatt atttatatca
    11161 tatttatcta atcctttaaa agcagttatt tcatcattaa gctcatcact accatctaca
    11221 aaaacacaac ctgtttcaaa ttccatacct acaataatct tacagccact ataagtttca
    11281 tcttgagtat cgtacaattt tattgcattt ccttcatttt gaattgtaaa tacaatagat
    11341 tttaattctt tatcactggg aatcttttgt gaaaaaggct tgtttaactt aatattattg
    11401 ataactacat gtttaaattc ctctgaagtt tcttctacta ttacatattt tcgttttaaa
    11461 aactctatta tttctaaaac agacttagaa tttactttta aaatactttt atatttttca
    11521 tatacagatt tccactgttc aattttttcc tgtggtactt cttggatatg tgtacttatt
    11581 ttttcttgag atattttttt caattaaact ctcctttcaa tacttaaata ttcttatgaa
    11641 tctcacatga ctaaagttat aagattccta cttaatagaa ttttgcatac ttaaatatgt
    11701 cttattaatt ctctgcagga tatctccgta gttcctatga ttcttacata aattacctat
    11761 tttaaattgt tattagttag attataaaaa ttatttattt tatcactaat ataatactct
    11821 taaaaacttt acaaatcaat ttaaatttgc taccttttat ttataacaac ttatagtaca
    11881 tttctcctat gcttttcaat atattacttt taaaaggagg ttttccttat gattttaaat
    11941 agaactgagc ttttccgagg atattcttta aatagattta acgagatatg caatatatta
    12001 tcagtaaata aaattaaata taagtataaa gtgaaaaata atgcttactc taaaaaacct
    12061 ttttttaatg aaataacact tggcaattta ggccaaaagg aagatttctc ttatgagtac
    12121 ttaatttatg ttcataaagg cgattatgaa caggcagaag ctctaataaa ttctaagttg
    12181 cgatagatta tttataaaac atgacattct gttgtcatgt tttatatgtt attatacttt
    12241 caaatatact actatatttt caaaattgtg tgctttaaaa ctaccttttt atagtaattt
    12301 aaaacttaaa actaaggaga cttgttatat gaaaataaat ataaaatcaa cattaaatga
    12361 atttattaaa cagaataatc tttttgatga caacagaata atatcctacc ttcctaatat
    12421 aacaatagac acagacaaaa taaaaactat aatgattaat gaagttgtcc caactaaccc
    12481 caatgacgat ttttatagta tagataaaga tgctgattat ttaaaaacta ctattccttt
    12541 atttaacaat gcaggaatag tagtatctaa cataaatgat atcctagata tgggtatcta
    12601 tataactaat gccgtaaaac ttcctaaatc agaatataca ataactcgtg atacaattaa
    12661 attacatatg cctattttag aagaagaaat taaattgttt aaaaacctag aggtagtaat
    12721 gttaatggga gatgttgcaa agaaatcttt taatatgatt actaaaaaac atataaaaaa
    12781 gaatgtcatc ccttccattt caacatataa acttcgtaat aatgaacttt attatgataa
    12841 tttaagagta tttccatcat atattatgac tggtggaaac atactaattg aaaaatcaaa
    12901 atttgaaatg gcatcagagg atataaaaaa aatgttcgag attataaacg gttgatattg
    12961 caaaataata aaaactaatt tagacaaata ataagtgcct caaaaacttc tatttatgag
    13021 gcacttttat tttatattag tgcaactata tactattaac ataatttgtt atttatattg
    13081 ttctttttaa tctctattcc atttagtcta ggtaacatta ctctttacaa gattacttta
    13141 tatttctctt atatctctaa catttttatt taataaaaca attattaata caagtcaatt
    13201 atatctattt atctaaaaat atatctttca cagtaatatc tggatggtga tatttcttcc
    13261 tatccgttaa aaaagatttt tttacattaa tagcctcttt tggacaccag tgaaaacaag
    13321 ctaggcatct ttcacactca ttaccccact ttggcttttt atcaactata cttatattct
    13381 ctacaggaca aaccctttta catgtcccac atccattaca tttattttct gcaaaaaatt
    13441 tagtagttgc aatttgcata ttcttaccta ctaaacgaag tacaacacta tctatatgtg
    13501 actttaaatt gttttttcct tctatatttc caatttccaa gttattgata cattttgcaa
    13561 tctcagctac cctattagtc tgatttctca atctttcttc ttcaacatct ggaggtgtta
    13621 tcaaagcatt tccaggcata ttaattaaaa aaccagcaga taattttttc tccttttttt
    13681 taagtaactt atctatagta aataaactat gtccagctac cccattacaa gtggcaatgg
    13741 catatatatg agtattaata ttaaaattta atttctctac aaaagacttt actatatcag
    13801 gaacatcagc aaaataaaca ggaaaaacaa ttaccaatat atcttcattt atgtctatat
    13861 tattttcttt tctatgtata gctaaaggta taacttcttt actttcattt aacatttctt
    13921 tcaaagtctt tgaaactact agcgaatttc ctgtaccaga aaaataataa atcttactat
    13981 tcattcttat ccctctttct atctttctgc atcatcaata gttaaaaagt ctcttaaaat
    14041 aggatttata tttactttcc aattctatcc aaaatatcag taaacaaagg caattcttca
    14101 tcagagatag atttaaatgt ctctgttgca tatacatttg caattttatt taatggttct
    14161 tctaattctc tacctaaatc tgttaggaat atttgaaact tacgtctatc tttaacatct
    14221 actttacgga caattaatcc cttatttttc ataacatcta agattcttgt cgtattagat
    14281 ttatccttac atgatttttc acataaatct atctgagaaa ttccatcttg ctcccataga
    14341 aaccttaata acgcataatg gtcagttgtt atatcatatc cattttgttt aaacatcttt
    14401 tttaaatcat cttttacttt caatgaagta cttactaact tagttgtagc ttcatatagt
    14461 ttctcaaaat ctttcatttt acctccattt aaataatccc ttgcgcctcc catctatcaa
    14521 ttattccctt atattgctca atgtctttaa aagccattgg ctttaaaaca gcatttatag
    14581 caataaaatt atagatttga gaaaacacag gtggattttt cttttcctct atgcaataaa
    14641 taaatttttt ggataccttt tgtatctttg attctacttt ttcagttata ggattttgat
    14701 taaatccagt ttttatagat aaactaccac aaacataacc tcccgaatct aagaccattg
    14761 attttataaa agaaattgct tttgaaccac gagcattttc atatgtagta actgctatac
    14821 atggcttacg atacaataat ctttcaagca tcatatgtgc tctatctgta aacactttga
    14881 aaagtccagt tacactactt ccataagttg gactaccaaa tataacacca tcactcttag
    14941 taattatatc atgaatatac tctactttat cattttgatt aatacacttc cccattttat
    15001 aacaatttaa acaaccaata caataggatg gattgacctc acttaaatca aaataattaa
    15061 tttttacatc tggtattaat ctttctatat ttttatacaa ataagtcaaa acttttgatg
    15121 tagctccatt ttttctaggg cttccattta taactgtaat tatcatttct atctctcctt
    15181 ttttagttgt tatatcaaca ataagtatat caactatatt tttagctgtc aatataaacc
    15241 ctttatttaa agctatactt acacagctta aataaaagtt ttatttaact acatgtgctt
    15301 atttaatcaa ctataaatga attattttta ataattgaca aactaaatta atgataatat
    15361 actttatgta ttacaaatgt aagtagaagg aggtatagca tgaaaaagtt accacatatt
    15421 tcagaagcag aataccaagt tatgaaaatt atttggaaat atgctcctat cagcactact
    15481 gaagtaattg aaaagttggt agaaacaagt acatggagtc ctaaaacaat tcaaacaatg
    15541 ttattgagat tagttaaaaa aggagcttta acatatgaaa aaaatagcag agtattcgtt
    15601 tatactccac ttgttaaaga agaagaatac gtagctacag aaagtagttc ttttttaaat
    15661 cgtttttata atggtactct taattctatg gtactaaact ttttagaaaa tgataaacta
    15721 tctgaagatg atattgagga actgagagaa atcttaaata aaaggactac taagggggat
    15781 aaataaatat gtatgcccca ttctttatac gatttttact cagtacatta accttatcaa
    15841 tattaattgt tgtaatttta cttgccaaaa aagtatttaa aaaacatatt tctgcaaagt
    15901 accaatacaa tattggattt atattgctta taatgttaat acttccattt gtccctctta
    15961 aatatattaa tcttggaaat gtatttaact atttatatac ttttaatcaa cttggaagca
    16021 atactaatag gacttctatg ataaaaaata gtccaaattc tataattaat aactcaaatg
    16081 cattaaacga tttttcagta tccctaaacc aagttaattt tgatagttta aatattttat
    16141 tactaatatc atggataatt ggaattttaa tcatgatagc tattactatt cattgcaaca
    16201 ttaaaataaa aaatatgaaa aaatcaatac aaattttaag agatgaggag atagttttta
    16261 catttgaaaa gtgtaaaaaa gatattggta taaataagga ttttatacta tgcaaatcac
    16321 ctattataaa tacacctatt acatttgggt actttaaacc acacattata ctaccagata
    16381 agtctatatc taaattatct ttaaaagata taaaatatat cttacttcat gaattacagc
    16441 attttaaaaa tagagatata ctgataaatt atataatgtg cttttttcaa atattatatt
    16501 ggtttaatcc tcttgtatgg tatgcattta aagaaatgcg tatagaccgt gaaattgcat
    16561 gtgatatttc agttttaaaa aagcttgata aagatagtta tatagaatat ggacaaactc
    16621 taataaactt tgtggataat atttcaagac catcaaatct tacacttaca tcagatattg
    16681 gtgggtcaaa aaaacaaata aaaaaacgat tattgaagat aatggatttt catattgaat
    16741 caagtgcttt aaaattcaaa agcattttaa tttttacatt ggtagcttta attgtatttg
    16801 gaaatattcc tagactatct attatagcag atatcaatga aaaatataga cctacaaatg
    16861 acaaaacggt gtatgaggac ttaagtcaat attttaaagg ttttgatgga agttttgtta
    16921 tgtatgattt aaaatccaaa caatatcata tttacaataa agaaaaaagt aaaacaagaa
    16981 tttctccaaa ttctacttat aaaatttata gcgccttatt tggattggaa aatgggataa
    17041 taacaagaga taattcacat atcccttggg atggaaaaca tcattctaat agttcttgga
    17101 atgaagacca agacttattt tctgctatgg aaaattccgt aaactggtac tttcagactc
    17161 tagataaaaa aacaaagcta aaaaacatac aaacttatct taagcaaatt ggatatggca
    17221 atcatgatgt ttcaggtggt atctcaaact tttggctaga atcttcatta aaaatatcac
    17281 ctattgaaca ggtaaaatta cttgaaagtt tttatacaaa taagtttaaa tttgaagata
    17341 agaatataga agctgtaaaa gattcattgt taatatcaaa aaaggatggt gtatcccttt
    17401 ctggtaaaac aggtactgcc aatataaatg gaaaagatgt aaatggttgg tttataggtt
    17461 atgtggaaaa aaatgaaaat acttatttct ttgctacaaa tattcaaagc aaagattatt
    17521 ctaatggtag ttcagcatct aagataacat tatccatatt aaatgacaaa aaaatctata
    17581 atccttaata tatttactgt acattctaaa aaattcacat aattttcata ttaattatcg
    17641 attaaattca tatacaaggc ttattatata aacatagaag atagtaaaac tagcttaaaa
    17701 acataatata ataccctata caaatagtgg caactctccc tcacatcctc aattgttgcc
    17761 actatttaat tttttgtatg aagttaaata tattttcatc atttcaacaa taaaaaagag
    17821 gtctatctct attttgaaac aacctcttta attattttag agtttactaa gaagtctaaa
    17881 aacaatgtat ctaaaatatt attctcaact gagtaagatt aatttccaaa ttgagtaaat
    17941 ttaatcttca aatactgatt taacaatagg aataacataa tgacttcccc cacgactctt
    18001 tacatcttca atcatagcaa ttattagata ttgtttatca ctattttcat ctacagtaaa
    18061 agcattaaac catccaagtt ctgtccctgt aacatcatct tttgatgctt taatttctgc
    18121 tgtaccagtt ttcccagcaa tgctaattcc tttaatcata gcactatgac ctgtaccatc
    18181 agggctttca acaacttgaa gtaagtcatc acgaataatt tttacagcct ctttagaaaa
    18241 cactcctttc ttccaaaatt cagccttttt aaattcaaga tggggtttta tcatgtctcc
    18301 atcatttaca aaagctgaat atattgatgc cgtatgtact ggatttacaa gaagcttacc
    18361 ttgtccatag ccagtatctg ctaattgtat ttcagtctca aatttattgt cagtgccaaa
    18421 ttttgaagaa gacaatccaa attcaaatgg catactttca tcaaatccaa gcttcaacaa
    18481 ctcttttgct aatacatctt cacctatttt taaggctgcc ttagcaaaat aaatattgtc
    18541 agaataaatc aaagcatttt tcatatttgc agtatttcca taatctttta acgtggttac
    18601 cttataactt ccccaactag aatctttttg ccaacttaga ctgctgtgtc caaagttttc
    18661 atttggatta atctttcctg ttgttatacc aataccagca gtaactggtt taaaagaaga
    18721 cccaggacat aatcgtgctt taaatctatt gtacattggt ttattttcat ttttattgag
    18781 ttcattccat ctatcttctg acattcccaa tataaactca tttggattgt acgacggact
    18841 acttacaagt gataaaacct ctcctgtttt aggattcata actactacac aacctttatc
    18901 atttttcatt tggtcatata gtttactctg tagttttgaa tctatagtta gctttacatc
    18961 ttctccatct acctttggct cagttactaa agtttctttc ttgtcaccat atctatcagc
    19021 tattataatc tcatatccat caataccacg tattctatct tccagtaaat tttctaaacc
    19081 aatcttacca attatactat tatcgttgta ccttttaccc tctttttcct ttagtatatc
    19141 tgcatttaca ttttgaacat aaccagttaa atgaccagtt ttttcaccaa atggatatac
    19201 tctaacctct gtattggata ttttaactcc aggaatacta agaagtgact cttgtagttc
    19261 cttatcttct tcttttacag taccatctgt attttctttt attttttcaa tagtttttat
    19321 aggtaccatt gaattttctt ttacccaaga tgcatttaat ttcttttcaa tttgctcaac
    19381 tgatacattc aataaagtag ataatttttt taagtcttct atattatcac tcatttttcc
    19441 aggaactaat cctatcatag atgcaagacc ttgaccagca atcattacac cgttcctatc
    19501 cagaatacgt cctctcttac ctttcagctt tgataccctt acagtattat cttcttcaag
    19561 ttctggaaat atgacgtttg actgccattt tattttataa tttttctgtc tatctctagt
    19621 taatagtact tcatttgaaa atgaaatttc acctgctaaa gtatccatct tagaatcata
    19681 gtttattact ttatcattat ttttattttt tttaatatca ttaatcttaa tacttatatc
    19741 gtgtgcatca attccttcat agattttttt atttcttaaa acaaaatctt cttttttatt
    19801 ttcagacttg ctcttttcat ctaataaatc atacattttc tcatattgtt ttttatttgc
    19861 aaaagacata tactgcttaa acacttcatc tggtttgttt ttattgagtc catctagtat
    19921 gaattttgct aaaaaaacaa taataactat aagcagacac attagaaaac caaaaattat
    19981 catttttctt gtttttttat tttttctcat ataaaccacc tttatcattt aatatattac
    20041 atacgtaagc tttaaggtta aaatactctt atagacatag ctaatctatg tttttattat
    20101 gtttttaagt atctcataaa aatatgtaag cagtataatt gatagtaatt tatatattac
    20161 acatgtaagt tttaaaagtc aataatatta tcattattta attatttttt acatcaactt
    20221 aaaaataaat cagttccatt atattctttt gtacccttca actattttat aaattaacct
    20281 tattctattg taattatttt atggtatata actttaactt gacaaaacga cacaagtgtc
    20341 tcataattaa aaatgacact tatgtcgtta aaaaggaggt taaaacatat gtcaacaaaa
    20401 ggagaacaaa ctaaacaatt tatcattgat acatcaataa tgctattttc tgaaaaagga
    20461 tatagtaatg tgacaatgaa agatatatgt gaagcatgta atttaagccg tggtggccta
    20521 tatagacact ttaattcacc aaaagaaatt tttattagta tattaaacgc tcataaatac
    20581 tactctcaaa atttgttgga taaaggtatc aaggaaaata tatcagctaa gcatatgcta
    20641 gaatattttt tccaacaaca aaaggctgac ataaatggca aatattgtgg atttgatttt
    20701 gcaatacacg aatttgcttt tgtagaaaaa gaccaacgtg attatatgaa taaaagatac
    20761 agttcagcta tcacaatgct ttcaaattta atcgattatg gacaatctaa aagtgaattt
    20821 aaaaaatgtg attctaaatc agttgcaata cacataatgt tcttatttga tagcatgaaa
    20881 acatctgctt ccattttaaa acttacaaat aaagacattg ataatcaaat aaatattata
    20941 aaggacatgg tgattttata tgaataaaca aaaattccat aaaattgatt tacaaaactg
    21001 ggaacgatat acacattttt actacttttc aaaaatgaca aatgtagggt ttaatttgac
    21061 tatagaaata gatattacta atttgcttaa ggaaacgaaa aaaagaaata ttaaatttta
    21121 tccagcgtat ttatatacaa taactacttg cttaaatcaa tatagagaac taaaaatcgc
    21181 atatgatgaa gatgaactcg gatattggaa cacgttaacg ccactttatg cgatttttca
    21241 tgatgataat aagactttct catcaatatg gacagaatat gatgaaaatt tctcaatatt
    21301 ctatgaaaat tatttagctg atgttaaaga atatggaaaa aatcatggtt ttctagcaaa
    21361 accaaatccg ccaagaaact gctatactgt ttcttgcctt ccatggatta gctttaagag
    21421 ttttactgta cataatcatg gtcttgaaaa ttattttttc cctacaattg aagcaggtaa
    21481 atactatcaa aaatatgaaa aaatacttct tcctctttct ataacagtgc atcacgcaac
    21541 tactgatgga taccatgtaa gttgttttat tgattacata caaaaaatgt gctttaatac
    21601 taagatatgg ctacactaaa aatttataca atataaaaag aactgtcgca tgagcaaatt
    21661 aaatttgcta tgagaaagct cttttttact taccaatagt taatatgaga tgaattattt
    21721 cttatacttt gaacttacca atagttaata tgaaatgaat tatttctcat actttgaaaa
    21781 tattgaattg attttgataa tcttctttta taatcattac gtgtcaccta ctactcaaca
    21841 catattcttt gacctatttt tacatccttg tttatatcca aaatatttac cttcttacca
    21901 tctggaaaca ataccattgt caaagtagac tttcctaatt cgtgtattct ctttaaatca
    21961 acattaacaa gaacatcacc ctgtttaacc atatctcctt gtttaacagt aacatcaaag
    22021 ccatctccat ttaatgcaac tgtatcaatt ccaacatgaa ttaatacttc tccattagtt
    22081 cctttaatcc caaatgcatg tccacttgaa aatactgttt ctaatttacc tgaaataggg
    22141 gaaacaattt ttccatctat aatatcaata gcaaaaccat cacctaacat ttttttagaa
    22201 aaggtttcat caggtacatt ttctaatgca acaatcttac ctttagctgg agacacaaag
    22261 ttttcattat tttcattaaa cactaattct tcgtcttcat cttcatttat agcttcttta
    22321 ggtactccaa ataaacatgt tgcaataaag cagaatacaa ctacaaatat tgatactaat
    22381 aagtatgtga ttaaattata tccatcatag atatacatta aataagaagg taaaactgct
    22441 aatccatatg aattagattg aatatgtaaa attgataata ttgctgctcc taaacctgaa
    22501 gatgcacaca taataatcaa aggctttaaa ttatatcgaa gctgaatccc gaataaaaca
    22561 ggttcactta taccaaaaag ttgagatagc attgctccaa ctgcagtttg tttaacttga
    22621 ttatttttag attttttcat aaatgctaga catgttccaa tattcgcaaa tccatacatt
    22681 gcacaaagag taataagtgc attaaatcct gtatttgcta atagtgatgt ttcaatcatt
    22741 acataagtat gatgtaagcc agtaataaca gctaatggat atgtaaatcc aataataaat
    22801 cctccaattc ctaaaggtaa accaatcaat aagctgatga tatcaaccat ttttaattct
    22861 attgtatgca ttataggccc tacacctaac aaaataacta acattgttat tagcataaca
    22921 aagaatggtg tgaaaataag gtctaataca ttaggcatat gtttacggaa ccatttttca
    22981 agattagctc caataaatgc tgtaacaata gctgttaaaa cacttccttg acagcctaca
    23041 ataggaataa atccaaatgc cataattgct tcaacttcat ttgatgggtc agcaacagaa
    23101 tatgcattag gaagtattgg ggaaactaac ataagtccaa taacaagtcc aataacaggt
    23161 gttcctccaa ataccttaaa tgctgaccat acaataattg ctggtaaaaa agcaaatgct
    23221 gtttctgtaa gaaccgaaac aagtgtctga atatacaatg gaatatttgc agaagacatt
    23281 ccaaataatc caagtacatt atcattaaac agacaaccct ttaatcccaa gaataatcct
    23341 gttgccgcaa taactggaat aataggaaca aatatatctg ctagagttct cataactttc
    23401 ttaaatccag tttcattatt tttaactaat tcatcttgtt ctttttttga taaagttttc
    23461 aatcccattt tttcaacttc tgcataaact ttattgacta ttcccgttcc taaaataatt
    23521 tgatactgtc cagaagtaaa gaaaacacct ttaacctcat taaccttttc cactttctta
    23581 tcattaataa tttctctatc tttaaccatc acccttaatc ttgtggcaca atgtgtaata
    23641 gaaataatat tattttcacc aataatttca ataagctcat ttgctatttt attatattta
    23701 ttcatttttc tacctcactt tcaataatac tgcttgaaat ggtttcaaac tcactgtttt
    23761 atttagtaga tttatatcat ctaaattttg gaatacaata tcatattttt cttctgtttt
    23821 tatttctaca ggtttatcag aaaaattata ataacaatca ataactacca aatctaattg
    23881 ccttctatac gcaataacat tgtcactttc tatatcaata ggttctattg aaccataaat
    23941 caaacaatca gaatactcac tatcttgtct caactttatc atctttttat aaaattctaa
    24001 aatactttct gaattgttaa tctgactctg tgcattaata tctttataat tttcaataac
    24061 ctttaaccat ggtgttgtat ctgaaaaacc tgcattttct ttatctgacc atggaaatgg
    24121 cgttcttgca ttatctcgac ttcttaaatt cacaaattct aacgcttctt cttctgagta
    24181 accttcatct attgaacgat agtattggtc aatgctagat aaatcattaa aatcatttat
    24241 ggtatttctt ttaaaatttg tcattcctaa ttcctgtccc tgataaataa atggtgttcc
    24301 tcttaaaaag aaatacattg ctcccaacat ttttactgca tcatcattcc tttcgtattg
    24361 caaaagatac tttgttgtag ctcttggttg gtcatgattt tcaataaaat tagctgccca
    24421 tccatgttgc tgaacataca tttgactatc taaaatgtat tttcttaaat ctgaaattgt
    24481 ccatgaaatt cttttaaacc actcactacc agaagcaaca tctaagtcag catatttaaa
    24541 gtcaaatacc attgaaaaaa aaccatcttc tccaataaat tcacctagct ggtcatagct
    24601 aatacctgct gcttcagcaa cagtcataca atcatatttt ttaaacgtat tttcttttaa
    24661 ttcataaagc attttttcca ttccaggctg atttctaact gcttttgtac atttaacaag
    24721 accatcaaca ccatcacttt ctaaactttg aaaacttaaa ttctttttga tgaatgtaat
    24781 agcatcaact ctaaatcctg ccactcccat ttgagaccag gtattaatca tttggtaaag
    24841 ttcacttctt aatattggat tttcccaatt aaggtcaggt tgtttttttc caaaagaatg
    24901 aaaataatat tcgtctctat tctctacctt ttcccaaaca cttccgccaa aaacagacct
    24961 ccagttattt ggcaaatcgt ttcctttttt aaaaatataa taatcatgat aaggactttc
    25021 tgggtttttt aatgcttctt gaaaccaagg atgttcatca ctcgtatgat taagaactaa
    25081 atctaatata atcttaattc ctctatcttt actttcttta attaactctt ttaaatcttc
    25141 catacttcca aattcacttg caatatccaa ataatcacta atatcatacc cattatcgtc
    25201 cataggtgat ttaaaaattg gacataacca caatgatgta atacctaaat cttttaaata
    25261 atctaaacga gaaacaattc cctgtaaatc tccaattcca tcattattac tatcttgaaa
    25321 gcttcttgga taaatttgat aaacgatttc tttttgccac catttctttt ccataaatta
    25381 ctccttttta tatgatattt cttcttttta attctttata aattccatta tcgaatatgt
    25441 cctcacatat agatgttgct atatctttaa gctgttgatg actatttttc attgcaattg
    25501 taatatctga tgcttgaaac atgacgatat cattttgacc atcaccaaaa catatggttt
    25561 ctttttgagt tatccctaat ctttcttgta atctttgaat tgctttgccc ttatgaaaat
    25621 ctttttgaat aatttcataa tactgactag aaatatccct ctgcgctagt tccattttat
    25681 cttgtagtat atctttcacc tcatcaaata ctttttcatt tgaccataaa caaattttat
    25741 gtatatcttg taacttatac tcttcaatgt tattttcata tgttattttt tcttggatat
    25801 gttgtttatt tatacaagaa ttagttcctt tcaactgatt cattgtttca aatatttctt
    25861 tggctttttg attcatgaaa actttttcct gactctcaat cgaaaaagca acttctctct
    25921 tttttaataa acaaacaact tcctttatca atctttgatt aaaagattga ttataaagta
    25981 attcaccatg atattgaatg taattacctc ctcctgcaat atatccatca acccctaatg
    26041 ataaaacatc atcttgtatt gtacccatac ttcttccagt acaaataaca acactgcaat
    26101 gattcttttg acataaacgt atagcatgct ttgcagattc tgggattcca tatacctcat
    26161 ctctaagcgt accatctata tcaagtatta ataatttgta caatttcatc ttcctttctt
    26221 actatcaaaa tcataatagc attcaaaaaa aaagatatca atattttgat atcttttaaa
    26281 aaatatgttc atttttagca caaattttca ttttcgggga atatgttcac tttctaaata
    26341 catcttgtat ttaatataaa gcagttcaat taaaataaaa taaggtgttg ttacatcgta
    26401 gtccagttgt ttatctacag aaatagatat agattgaata tataggtttt catcacataa
    26461 tcttgctaaa ggattactta tcatttttgt aatagaaacc atataaattc ctttcatttt
    26521 cattttttta gcaaagtcaa taattgtaga tgcatctcca tttaatgaaa taaagaaaat
    26581 catatcatcc gtttttataa aatgattaag tgcctgaatc atatcatagc cataaacatc
    26641 atatataatt ttccctgttg gtaagaaaat ccttttcatc tctttagcaa ctctttcctg
    26701 agaataacct tctccaaaga cgattatacg atttgccttt ttcattcttt caaacataat
    26761 cgaacaatcc ttcttctcta tatcatctat aaccttatta taacaatcca ttaattggtg
    26821 tttactttga tttttatatt ctacttctgt ttcatttaag cgtataataa ctcttaattc
    26881 actatatcca ggtaattgta gtttttgagc aaaacgagaa agtgcacttg tagatatatg
    26941 gtatttgtct gcaaattcat ctatagaaag tttaatacaa tcttctttat gattaagaat
    27001 acactcacag atatacttat catttgctgt aaatgaatta taatatttat tgaaaagtcc
    27061 ttcaattttc attcttttct ccttctatac tctatgtaat tcatatgaag caaatctaat
    27121 agagcataaa atgctcctac gacttcataa gatatagctt gtaatgtatc aagtttttgc
    27181 gtttcaacat acagattata ctcacaaagt gttgcaattg tattattatc aagcctagtt
    27241 aaagaaatcg tatgtatatg attgtttata aattttgcaa tttcaattcc tgctttcgtt
    27301 tctccagaca aagaaatgat aataaataaa tcactttttt gaaatgattc tctcataaat
    27361 tcaatttcgc ctatatcaaa taagtcaata acacatttac ctactgaaag aaatattctc
    27421 tttatttctt gagctaaact tttctgttca ttacctgtcc cataaatata aattgtttct
    27481 gcataatata ttattttaca aatatcttga aatgatattt tttgtaattc attcattaat
    27541 agtcgataat tctcgcaaac atcatgaaaa ttattatcat tattgacaaa ttctagttga
    27601 ttatttttaa gtaaatactt taaatcagag aatgaatcta atccaatttt tctacaaagc
    27661 cttaataaag tagctctaga aacattacat tcatttgcaa ttttttcaca cgaaaaatta
    27721 cggtattctc tttgacaaat caatatttgt ttaataataa ttttatcatt ttcatttaaa
    27781 ttatcataat tttggttaac taacgattct aatctcattt tttccactcc ctaaatatta
    27841 ttttaatata cattagttgc tttttctagg gaagagtctt aaaaatattt ctattaagac
    27901 aaataaaaaa gagctatctc agaacaaatt taatttgctc ataccacagt tctattataa
    27961 ataaaaaaat tttcacatta ttttttacac tatataattt ctatttttat aattaaaccc
    28021 aatttataat atatagaacc atatttatat ttttatctcc tgatttgttt taattacatc
    28081 tattcatttt ctatcttatt taataattcc tattacttga atatttactt ctttgagtaa
    28141 atttcatagc taacaaagct aaaattatta ttataaatgt caaaatacaa atataattca
    28201 tattaaaaaa gcttccatct ctttcttcta tataagtcac aaaactaata aaccattgtg
    28261 ttgggcttat aatactaatt ttttgtaaca ttgttggcat tttttcaact gaaataaatg
    28321 aaccaccaat aagtgataat ataagtgata tacatgtaga aaacaaattt gcatgcattt
    28381 ctttattgaa taaagaagat acaaatgttc caaaagaagt tgccaaagct gatatccata
    28441 ctcccattaa aattaaatct atataatcca taccaatatc aatattaaat aacttaatta
    28501 tagatattga aactatgtac tgaaaatacg ttattgtaaa gttgtataac atattaccta
    28561 gcacatatcc tcttatattt tgtggagaat atagcactct tgtaaatgta cctgagtttt
    28621 tatctttaat aatcattgat gctgttacag tagatgttat cattaaaaat aatacgataa
    28681 atgcaattat tctttgtaaa actcctaaag tattttcttg taatttactt atatctacag
    28741 gaactgggtc ttttttatat gtttctatta aatactttaa tgtatcattc gtatttttat
    28801 cttttataga atataactca aaaccattct tattaaaaca tatgacagct gaatacttgc
    28861 ctgttataat atcagtttta attgttttaa aatctgcaat ctctgtatta atcccttgag
    28921 tattttctaa agccattatt gtattttcat aggttctttt ttcagattcc tttagaatgc
    28981 attcgttttt gtactcatct aaaataccaa tattaaaaga tggtttactt atgtaatttg
    29041 ccaatactcc taaaactact ataattatag gaaataaaag tgtaactaaa aacactattc
    29101 tcttatttat atttcttttt aagttactat taaaaacatt tattacattc actatataaa
    29161 gacctccttt ttaaaaaatt tgattgtaag tattgtcata aatatgctta cagctacaaa
    29221 tatgcatgat atattaaaaa ggagttgtaa attattatca tagatacaag caactatgcc
    29281 tctattaatc catgttaatg gtgatatatt aatgattaat gaaataactg gattcaatga
    29341 actaactgga aagaacactc ctcctaagaa gccaaaaata actatcggta tattaatgat
    29401 attacgtaac atatctaaat ttttacaaac aagacccaaa aacaacccca tagatactac
    29461 tgcaatagat tcacacatta aaaagaaaat aacaactaaa aatttcccac caaaactcat
    29521 atgaaaaatt cctcttaaca ccactaaagt tatcaaattg tatatcgaaa atactgttgt
    29581 gcaagaaaaa agttttgaaa taactaaatc tactattgaa ataggagaaa tcatataacg
    29641 atatgatgtg ttagaaattt tttcatcttg tgctacatat gaaacactaa taattcccat
    29701 aagaacacaa aatggtattg taacaatagt ataataatta tatgatgtaa attccttacc
    29761 ataagacttt gatgtcaaat atcctaatag tactatgatt attactggaa aaacaatatt
    29821 gtaaaacaga ataaaaaaat ctttacttct tcgttttatt cctaatttaa acaatgttaa
    29881 aaaacatctc atctatttat tcctccaaat ctctcaaatc tgtacctgta agatatagaa
    29941 aaatatcttc taatgtctta caaccaaatt ttaaatattt atttttatat aaactcttgt
    30001 ctaaatcttc tataattact cctttatcta ttaaaacaat tctattacat aaagtttcaa
    30061 tttcctccat atagtggcta gtatatatga ctgttgtgcc attattatta atgcctttaa
    30121 ttgcttccaa aattttattt cttgattgag ggtctatacc tactgttggc tcatccatga
    30181 tgagaatctg tggactatgt gcaattgcac aagctatgtt tagtctacgt ttcattccac
    30241 cagaaaattt tgatggaaac tccttatgac tttcccaaag accaacaaat tccaaggcat
    30301 atttagttct ctctttaagt tcattccctt taaatccata taatgagcaa aaaaatttaa
    30361 cattatcata tgcacttaaa tcttcatata ttgctaattc ttgtggtaca actccaagtg
    30421 agcttttaaa aatattttgt tgattcttaa tatcttttcc tttatagtat atgcttccac
    30481 tatctggaga cacaatcgta gtaagcatat tgatagatgt gcttttccct gctccatttg
    30541 gtccaataaa acctaaaata tctccttcca ataacttaaa actttctctt gatacaactt
    30601 ccttattgtt atacttctta gataaatttt taatctctaa caatacatta ctcatttata
    30661 aaatcctcct aatatttaac caacattata tatccaatat tgtatatata gtttttttaa
    30721 aacacgagtg ttttactcgt gttattataa taaaatttat tacttatttt gtaatttcag
    30781 tattatctta tctacatttt ctatagcttt atctggatta ttaattattt catctctaag
    30841 tctttgaact tctaataact catttgtcgt atcagatgta aataaaaaat cttcttctat
    30901 atttgaaaag tctatagatt taattatatt ctgtgtctcg caaccaacag atatatactt
    30961 atcgatatta tttaatattt cttcatgcca caaaatttga taattcagat tatctatagt
    31021 catatcaaat gcaatttttt ctgttgcaag actcttttta tcaatttttt taatctcttt
    31081 ccaattttcc caatacttct tctgctctat aagatatttt atatgttttt caagattttt
    31141 ttgcaaagta tccttaggta atacatccaa aatattatgt aataggaatt tatttgaacc
    31201 agttggaact attggcatct gaagctcttt ttgtatttct tcccttaatt ctaatttacc
    31261 gctttgagta atctcataga ttttacgaat cctagctcct gttttttctt cttttaaaac
    31321 tctaactcct ccatcctttt ctaattttgc caatgcataa tatattgacc cagattgtat
    31381 tttagtccat ctatctgcac cagatacttg taaaaacttt tgaatctcat acccatgtgt
    31441 tggcttaata tttagataat ataaaattaa agtgcgtatc ataaaaattc accacctata
    31501 ctctatcttg gatattatat tatcaccaac attggatata gtcaatagat aacataggat
    31561 atattacaac ttattatttt tattatttaa tacaaggagt tccatgagac atacatatcc
    31621 attcatattt taaattatcc aattctctta tagatttcat catcttttta taatcccaat
    31681 tccatggatt aggataagga actatatttc cattcttatt tttgactaaa tcacctgcaa
    31741 ataatacatc ttcaaaaatc atgcagacat gccctggagt atgaccagga gtatggataa
    31801 tttttatatt cccaatctgc atattctctc cataaggttc aacatcctta atgagccttc
    31861 tggaaattac ctttccaata aacttcttaa acccaggtct atccttctct cctataataa
    31921 aaggtatatc ctctacatga gcccatactt ttgctcctgt tttttctttc aataatttaa
    31981 tattagatat atggtcaaca tcatgatgag ttaataaaat atgcttaata tctgtaagtt
    32041 taataccaag tgtttttaat tctttaatta tattttcccc cataaaagga agacctgtgt
    32101 ctataagaat aatttcttta tcaaacacaa taaaacaatt tgagccacga gtactatcta
    32161 acattaatac attttctttt atcttcataa ataaattccc cttatttggt attttaccta
    32221 tataattaat taataaagtt ataaatagtt ataatacttg atattaattt tattttttat
    32281 taaagtatac tataggtata caagtattat atttacataa gtataccatc agtatacttt
    32341 tttaccaata gaaaggaata atttatatgt cagaattatc aaaaagagat aaagaaaaaa
    32401 tacaaagaga aaatgagatt attgataagg cagaaaaatt attttgtctt aatggatttg
    32461 ataatacaac tatgaatgaa ttagcaaaag aagttgaata tactaaacgc accatataca
    32521 agtacttttc atgtaaagaa gatttattct ttgctgttgt cttaagagga tataaaagac
    32581 tttgggataa tgttaaaata gaaagtgcaa aaggcaaaac agggtttgaa aaaataaaac
    32641 tttcatactt tgcttttcat aaattttact ctgtagaacc ttctctatta tgccttatgg
    32701 gaatgattgg tattgtaaac tctgaaaaat cggatactga tatgctgttt aaagaaaagt
    32761 ttttttcttt taataaattt atgtttgatg aaatacaagg aatgtttgaa attggaaaaa
    32821 atgatagaag tattaggcat gatattgaaa tacctactct tatgtattca tcaatattta
    32881 cacttacagg attttttaat ttactatctg taacaggaaa atcatacctg aacaacttta
    32941 atatagatga ggaaaaattt attgaaacaa ctcttgctct tttaatagat tcaattaaag
    33001 cataaaccta agtttataaa ttaaagttta tatacttaaa tgataatttt ttctatctct
    33061 aatataaaaa atattaaaca taaaactaat aacttagtta atgaaataac taaatcactg
    33121 aaaggattaa aagtaaatgg ctaaaatact tcttttagaa gatgatgata caattacatt
    33181 tggaataaaa acttcccttg aaaagaaaaa tcacactgta tgttgttata atacgattgc
    33241 tgaagctaaa aaaaattttg ataaaactat tgatttaatt ctgcttgatt taaatctacc
    33301 tgatggtatt ggatacgagt tttgccaata tgttaaaagt atagaagata ctccagtgat
    33361 atttttgaca gtccgtgatg atgaaaaaga cattatacgt ggattagata tgggtgcaga
    33421 tgactatgtg gttaaaccgt ttttgctctc agtacttcaa tccagaatta atgcagttct
    33481 tagaagaact cattcaaata ctaacaatac tattacatgt ggaaatataa gcttgaataa
    33541 ggttgaagtc aaagcatatt tagataatgt tgaagtacct ttgacagcag gagaatataa
    33601 acttcttcta agtcttatgg aaaataaaaa tagagctatg actcgtacaa agttactaga
    33661 aatgctctgg gatgtagatg gtaatttcgt aaatgataac actcttactg ttacaataaa
    33721 gcgtcttaga gaaaaattaa aaaatccacc ttgtataaaa acattaagag gcataggata
    33781 tagaatggag gacagctagt gaaaactcca agaaatatca acattacagt tgtttttata
    33841 atattctgct taattgtaac ttttttattg agtgtaattc taacaactat aacttctttt
    33901 aatgaataca atgtactgtc taaggctctt ggtgcatcat atgagtacaa tcctaagtct
    33961 acagaaagta tactaaaatc actgaaaaat tctagtgata aggattttaa aacaggaaat
    34021 gaaattttat caaaacatgg atattctaaa agtacatttt ggagtaaaaa ctttttttat
    34081 tttctagtga tatcgtttgg tataatttca acaatggcta cagttgtata tataatcaaa
    34141 catagaacaa ataagcaaaa actgagtaga ataaacaatc ttactcaata cttagaagaa
    34201 gtgaatttag gaaaagagtc tgtactttta cgatgtgaag atgaattttc tcaccttgaa
    34261 gatgagatat ataaaacagt tggggaactt cgttgttcaa aagaatttgc tttaaaagaa
    34321 agacaaactc tttcagataa ccttgcagat attgctcatc aacttaagac tccaattact
    34381 tctatgtcac ttatggcaca attactttca gaaaattgtc cagatgaaga aatagattat
    34441 attaacagac ttacaaatca aatctcaaga cttgagagac tagtttcatc tttacttaca
    34501 ttatcaaaat tggatgcaag tactttaata tttgaaagca aacttataga tgtacattct
    34561 cttcttactt atgcaattga gcctatagaa ggttctttaa gagaaaaaca tcagacattt
    34621 actatcgagg cttctccttc ggtagaattt gaaatcgata ttaattggac tttagaagct
    34681 cttttaaata ttcttaaaaa ttgtagtgag catatagaag atggtggata tatagttgct
    34741 tattatagtc aaaatcctct atatgttgag attatcatag aagataatgg taaggggttt
    34801 gctcaggaag aacttccata tatttttaat agattttatc gtggtaaaaa tgctagtaag
    34861 gacagtattg gtatagggct tgctctatca aagtctatta tagaacgtca aaatggtact
    34921 ctacatgcag aaaattgcca taatggtggt gcaagattca taattaaatt ttacacataa
    34981 aatgctggat tataggtaaa atacctataa tccagctata tctatattct atgttatcta
    35041 ttgttttagt aatatacttc tatttacatt taaggtttta ttaaacaaac aaattaacta
    35101 tccctatact tgtaagtagt cttaaatcga aacctttaaa taagttcaaa tagattttgt
    35161 ttatattgtt tcatttttta tagattctac tatattttca ttcttcaact gcttgtatcc
    35221 tgtcatatat gatgctatta caataaaaag tatcaatata gtaaaaccta ttattgtaga
    35281 tataggtgca aatggtaaat actctataaa atatatttca tttatacgta gaaaagcata
    35341 cactattcct atttgcacag gaatactaag tagtaatggt gtaattccta agaataaacc
    35401 ttccaaaata agcatctttt ttatgccttc tggagataat ccaacagaac gcaacatagc
    35461 aaactcttgt cgtctacttc taatatttcc tgacactgta gcaagtacat ttgataatcc
    35521 aatcatagcc aataaacctg taataaagcc tactattaaa ttcatggtct ttattccact
    35581 agccttttgt ttttcacttt ctaaaacatc ctcaatacta taatctcctg aaccatagtc
    35641 tttattataa atggactcta atttttctct tactctagga atgtcctcac gattatttac
    35701 tttaaatact cctatcagcc tatttgaacg taacctactt acttcatcat aatttttaga
    35761 tatttcctgc accatagaca ttggcactat ttgcattaat gtatattttg aactagtatc
    35821 tactatttta ggaagtgtgc ttgtaatagc tcccactttc atgtcgtaag tataatcccc
    35881 tttatcttcg tcatatactt tttcatttaa tttaatgtta tctcctattg aaagatttaa
    35941 ataatctata tatactttat ttctacgtgt actacgatta atatcttcct ccttattata
    36001 tacaattgag gttggatttt ttgtatcata aaataacttt gggtctacag aaatttgcct
    36061 acaatattct gtaaaactct tatcatcaag cccaattata tttgaaatta tacgaaactt
    36121 gccatcacgt tctataggag agtatttttt agatgaaact atacttttaa atcctccatt
    36181 tttcgcaagt tcttttgatg catctttttc agtcagccaa gtggaagcag acattttttg
    36241 ataatatagt gaacttttta caccttctgt attatctaat ttttcaatga aagcagactc
    36301 tacaggttca ctattttcca aataaactgc tatatctctt tggtccttat aattatcact
    36361 tccataaaca gcttttgctc cttcttgatt tgcaattata tgcaaaaaac atgtaagtaa
    36421 taaaaaagat aatgttaaag atattgttgc agtacgataa gattttttac gagcacgtag
    36481 tgcgtttgat gcaagttctc cttctattcc aaatatcttt cctaatgaat atttatgcga
    36541 ctttttgatt ttaacactat ctccttgtcg tattgcttct ataggagata tctttgatac
    36601 ttttcgtgct ggtataagtg cagaaatcca gactgttact attgtaagta aaactgctgg
    36661 cagaaaagca ggaataccat aagtaaatac cacttctgga acatcagtat gataattaac
    36721 tgaattaaca taaactataa gcctattaca caataaccat cctaaaaata aacctattgg
    36781 taatggtata attgtcagta aaaagccttc aaacactaca gaccttttaa tttgttttgg
    36841 tgatgcacca acactagata atatccctaa ctgagtaagt ctagcatttg cagacagtga
    36901 aaacgcgttg taaatcacca ttacaaacac ggctactgta aaaattgcta ttgtaagata
    36961 cataatagga gttgaaaatt ttgatatact actcatagat gctttctgtt ctggtgaaaa
    37021 gactaacatc tttaaaagat aattactatt gtatttaaga ttgtattttc catattcatt
    37081 agtcttccat cccaatgatt tagcaagttt tggaagttcc ttatacgtgt ctcttatatt
    37141 tttaaatcgc aagtacacag ttatactatc attaggtttt atacttgttt tatcaagata
    37201 tccaagagct gtatatgcag gtattactga tgaagtagaa acatctacct ttcctactat
    37261 tgtcaatgta gcctctgaaa tttttttaaa actttcccct ttcgattttg gggctatggg
    37321 ttcaagaggc tttccattta ggattctatc tcctattggg agagtaattt tatcaccaat
    37381 ttttacactt ggattattct caaaatattg ttttgataat gcaatttcat tagcattatt
    37441 tggtcgacga ccctctatta tagtatcctt ttctggcata gaattccaat actctgatgt
    37501 agcatttcta taaattatat aatctctttt ctcatcatta attttgccta cataccattg
    37561 tccttttacc attacatcat caactgaaga aaaattttca attaatggta agtcacttcc
    37621 ataagtatag tcaaacaatt caccatgcca gttaccttct ttctcaattg aaagttttat
    37681 aaaatctgtc cacatggtat acaacagtcc acacaaagaa gacatcatgg ttgtagtaag
    37741 taatatagct aacattatag ctatactact acgtttattg cgtcgtacaa agtctaaggt
    37801 gtattcttga agaatattca tttcttcacc accttttcag agacaatcac tccatcttcc
    37861 atagttacaa tacgatttgc ctctagtgct attttctcat catgagtaat aagaagtatt
    37921 gtctgattaa atcgtttatt tgataagttt aacaagtcta caatttcttc tgaatttttt
    37981 ctatcaagat taccagttgg ctcatctgct aataaaatag ctggtctata gattaaactt
    38041 ctagctatag ataccctttg ttgttgtcca cctgaaagtt gacttggaag atggtttaat
    38101 ctatcagata atcccaatat agatacaatc tcagagaatc tatcctcatc cacctttctt
    38161 ttatcaagta acatgggtaa taatatattt tttctaacat caagggtagg tataagatta
    38221 taaaattgat aaataagacc tacttttcta cgtctaaaaa ctgcaagagc ttcttccttt
    38281 aaacttgata tttctgtatc ttcaacataa atctttccat atgtaggtct atctactccc
    38341 ccaagcagat gtagcaatgt agatttgcca gaaccagatg ctcctacaat ggatacaaaa
    38401 tcaccttttt gaactgataa atttacatta ttaagggctg tcactctggt ttgatttgaa
    38461 ccatatattt ttgtgagatt ttcacatttt aatatttcca tatacaatca tctcctttat
    38521 ggttaaattt tagcaaccta aaatgacatt atagtgacat gaatacaaat ataaattact
    38581 attaaaaata ttgtttagtt tttgcacatt atttaaaatt ataatataaa acaaaaacta
    38641 tgcaatatat tttatttata cttttaatat attacatagt ctttaaaagg tattaacttt
    38701 aaatctatag gtattcaatt taataatcaa tagcaagtat tttatgtact aaaggagttt
    38761 tgctctatat agactcaatt tagtaaaatt atttaataaa tattacagac tataggtaaa
    38821 taatatttac aaataaaatt aagtttattc ccttacacta taatatttct taattatatt
    38881 aattgcaact tcctttacct ttggccctgt aatttcttta ttggaatcat ctgatttaat
    38941 agcaaaataa taggtgtcac cttcacgctc tagcatacca acaaaccatg catccacaca
    39001 tttatttttt tcatcaaatc ctgttccagt ttttccatat acatttatgt ttttatcatt
    39061 tacatctatt ttcataatat ctcttaaaat attaatatgc tcttttttaa agtttgtatc
    39121 tccctcaaat atttttgcca ttgtttgcac ttgttctttt ggagatattt gtaatgaaga
    39181 ttctaaccaa aatccattta aatgtccctt tccatttttt aaatctcctt cccattcgct
    39241 aatatcgcaa ttcccatact ttaaatcatc taaaatgttt tggacagatt tagcatcaac
    39301 tttatctatc aattttttgt agtaccaaac acaagactcc ttaaatgcct cttctaaact
    39361 aagattttta ttccaatttt tatttggata ctctgtacca tcatatccca taactgattc
    39421 ttttgagttt attacccctt tttctaaacc aattaaagtt gaaactattt taaaagtaga
    39481 acatggtgac cttcttgttt caattaattc tttgttatat atactatatt ctttattttt
    39541 agtattataa aaaatagctc ctccactaat accttcaaaa caatcactat agtcaaccat
    39601 attaattttt tcttttttgt ttttattatt gttttcaatt ttggatttat ttaattcaga
    39661 cttatttaat tcagatttta tataattctt atcagttttt tgatttatat cgttatgttt
    39721 tttccctaca taatacatta ccataactac accaaccaat aagatagcta tccaaataaa
    39781 attctttttt cttttcataa tttatatcat ctcccatatt taaatttata acctactatt
    39841 aaaatagtag acattatttg taatatactc caatctaggt tctacttata agaaacaaga
    39901 ctaaatgaat agctgtatct cttatatttt cttcacctag tacaattatg ttgattaaac
    39961 ctattgcgaa gataattagg taactattaa taacatcctg tcctcctttt aatttgtgat
    40021 ataataattg attc
//
LOCUS       DAEORZ010000022        39454 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEORZ010000022 DAEORZ010000000
VERSION     DAEORZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.022"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            315..3861
                     /locus_tag="KRH39_001009"
                     /pseudo
     CDS             315..3861
                     /locus_tag="KRH39_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            4046..5980
                     /locus_tag="KRH39_001010"
     CDS             4046..5980
                     /locus_tag="KRH39_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBG8510930.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHRDKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVKKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6086..7081
                     /locus_tag="KRH39_001011"
     CDS             6086..7081
                     /locus_tag="KRH39_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8510931.1"
                     /translation="MKKYISIILLVVVTMVVLVGCSPGKDNSKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKDGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTIGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7053..7838
                     /locus_tag="KRH39_001012"
     CDS             7053..7838
                     /locus_tag="KRH39_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG8510932.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7826..8524
                     /locus_tag="KRH39_001013"
     CDS             7826..8524
                     /locus_tag="KRH39_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510933.1"
                     /translation="MEKIRLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8647..9120
                     /gene="ybaK"
                     /locus_tag="KRH39_001014"
     CDS             8647..9120
                     /gene="ybaK"
                     /locus_tag="KRH39_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBG8510934.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9317..10156
                     /locus_tag="KRH39_001015"
     CDS             9317..10156
                     /locus_tag="KRH39_001015"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBG8510935.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10169..10717
                     /locus_tag="KRH39_001016"
     CDS             10169..10717
                     /locus_tag="KRH39_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBG8510936.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10783..11964
                     /locus_tag="KRH39_001017"
     CDS             10783..11964
                     /locus_tag="KRH39_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBG8510937.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGSLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12259..12645
                     /locus_tag="KRH39_001018"
     CDS             12259..12645
                     /locus_tag="KRH39_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8510938.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12795..13058
                     /locus_tag="KRH39_001019"
     CDS             12795..13058
                     /locus_tag="KRH39_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG8510939.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13187..14029)
                     /locus_tag="KRH39_001020"
     CDS             complement(13187..14029)
                     /locus_tag="KRH39_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG8510940.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKAGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14324..15058
                     /locus_tag="KRH39_001021"
     CDS             14324..15058
                     /locus_tag="KRH39_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8510941.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            15068..16201
                     /gene="nagA"
                     /locus_tag="KRH39_001022"
     CDS             15068..16201
                     /gene="nagA"
                     /locus_tag="KRH39_001022"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG8510942.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGEVVYKNI"
     gene            16300..17049
                     /gene="nagB"
                     /locus_tag="KRH39_001023"
     CDS             16300..17049
                     /gene="nagB"
                     /locus_tag="KRH39_001023"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBG8510943.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17239..17496
                     /locus_tag="KRH39_001024"
     CDS             17239..17496
                     /locus_tag="KRH39_001024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510944.1"
                     /translation="MFSGILSSIIKLPIIGILPTGNIKNNTGISDSKKDGKQRRSNLG
                     ISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            18156..18860
                     /locus_tag="KRH39_001025"
     CDS             18156..18860
                     /locus_tag="KRH39_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8510945.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            18862..20349
                     /locus_tag="KRH39_001026"
     CDS             18862..20349
                     /locus_tag="KRH39_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8510946.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDANTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            20554..22167
                     /locus_tag="KRH39_001027"
     CDS             20554..22167
                     /locus_tag="KRH39_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG8510947.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(22530..23360)
                     /locus_tag="KRH39_001028"
     CDS             complement(22530..23360)
                     /locus_tag="KRH39_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8510948.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKTYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTIILKEHEEEYLVKTPIIYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPISIKPKGLYVTA
                     YHKGSYETIYKTYEKLLNFFNQNNLCMGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            23433..25160
                     /locus_tag="KRH39_001029"
     CDS             23433..25160
                     /locus_tag="KRH39_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510949.1"
                     /translation="MKKLIHFLKPYRVLIVIVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFVKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTVIII
                     VVMFLFMLSAFLVGKKLIKLFKMMQVKMDNMNRVLREVVTGVRVIRAFNRSHFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIIDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKYAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            25198..27060
                     /locus_tag="KRH39_001030"
     CDS             25198..27060
                     /locus_tag="KRH39_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510950.1"
                     /translation="MSEKRTDKQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQFIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRRAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKLDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFSSEYDEDVV"
     gene            27393..28082
                     /locus_tag="KRH39_001031"
     CDS             27393..28082
                     /locus_tag="KRH39_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBG8510951.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPTMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVATREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSASL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQVNKTTSTA
                     VSKYASSRLYI"
     gene            28171..28857
                     /locus_tag="KRH39_001032"
     CDS             28171..28857
                     /locus_tag="KRH39_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBG8510952.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            28874..30772
                     /locus_tag="KRH39_001033"
     CDS             28874..30772
                     /locus_tag="KRH39_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBG8510953.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKGVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLTSVA
                     NSNSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKVTKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKDFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSIVKT
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDENSSGIKGTEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNIDRNQGNPGGFGNRGVGSISKTTVYSKLILGGASMIIMSIMLVGVSRVK
                     RRRFIKSK"
     gene            31105..31794
                     /locus_tag="KRH39_001034"
     CDS             31105..31794
                     /locus_tag="KRH39_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510954.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLILYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVALQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            32230..32769
                     /locus_tag="KRH39_001035"
     CDS             32230..32769
                     /locus_tag="KRH39_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8510955.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            32788..33831
                     /locus_tag="KRH39_001036"
     CDS             32788..33831
                     /locus_tag="KRH39_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510956.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKESN"
     gene            33833..34669
                     /locus_tag="KRH39_001037"
     CDS             33833..34669
                     /locus_tag="KRH39_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8510957.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            34774..35448
                     /locus_tag="KRH39_001038"
     CDS             34774..35448
                     /locus_tag="KRH39_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8510958.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGTK"
     gene            35448..36500
                     /locus_tag="KRH39_001039"
     CDS             35448..36500
                     /locus_tag="KRH39_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG8510959.1"
                     /translation="MIKFKKVISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWDILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            36962..37810
                     /locus_tag="KRH39_001040"
     CDS             36962..37810
                     /locus_tag="KRH39_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8510960.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIAIPISSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            37800..>39454
                     /locus_tag="KRH39_001041"
     CDS             37800..>39454
                     /locus_tag="KRH39_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG8510961.1"
                     /translation="MMFKIFNKSLLKLVVILSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YDNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTNNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESDGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVEEIQIKNNGDEIIVNYDKDLDE
                     ITKSDARFKSISNISKILIFIVSISVLIFVIIFIYFKRIDKKK"
BASE COUNT        15570 a         4051 c         6887 g        12946 t
ORIGIN      
        1 tgattagata ggattttttt gttgtgaaat taattgaaga gtaattaatt agaaaaggaa
       61 tgaataataa tgaatatatt acattattac tttttgataa atcaaaaata aagttatagt
      121 ttttaatatt aattaagaat gatgaaatat gaataaagat tttacaaata aatcttaagc
      181 tatcttattt aattctaata caggaagaag gtaaatgtat attgaaaaaa tcatagaaat
      241 agaagtaaac aacttaaaca aatacaaagg tttgcaaaag aagtaatata attatataca
      301 atgaggatgt gagtatgatg aacagactaa agccaagaat tttaggtatt agtatcttgt
      361 ttattattat aattatttta acatgtggag ctgttttcaa gtttaaattt aaattagata
      421 ataagttaaa agaaaccaca catacatatt tgtctgagtt tacagaacac aatataatta
      481 atctcaagac aagacttagt ggtcaatttg acatgcttga atctattgct aacttcatag
      541 gaaacttgaa taatataaat gatgaaatgg tgattgattt aatggattct gatgtaaaac
      601 gaagttcttt aattagaatg actatctcta cattagatgg ggaatgttat tctaatgatg
      661 gaatcaccca taatgttcaa gatagagatt attttaaaaa agctaaaaat ggagaaaaga
      721 atatatcaga gcctttaaaa tctattgttg ataataaaga aataatagtt ttatctaccc
      781 cagtttataa aagtgggaaa atagttggtg ttttaagtgg aatttatgat tcaaaaaagt
      841 taaatgaatt attaggatta tctgcatttg atgaaaaagc agatgttttt gttgcaaaga
      901 aaaatggaga tataattgct caaaaaacaa ataaaaatag taagacgata tcatgtatat
      961 ttcagctttt tgacaaagat aattgtgatg aagttgattg tctaaaaact ataaaagaaa
     1021 atatgaaaaa tgataagtct gagtatttta attataactt agataatgaa aaatttatgt
     1081 taaactatca gccacttgga gttaatgatt ggtatgtttt ttctgttgtg ccaaataaaa
     1141 tagtgtctat tcagtcagag aatatatctt atgatgtata tatttttgca tgaaatacta
     1201 ctagttatat taatttttat aatatatata gcatgtatta taaataataa caccaaaatt
     1261 attataaatg aaaaagaaaa atttaaagta ctcactgata atatactagg aggggttaaa
     1321 acctgtttaa aagatgaata tatgactatg acatatgtaa gtcaggggtt tttagagctt
     1381 acaggttact cttgtgaaga tttaaataaa atatttaaaa atagatttaa agacatgatt
     1441 tataaagatg atttagacaa gtttaattgt attattctaa accaattaga aaaacaagat
     1501 aaaattgaag ttgaatatag aattgtaaga aaagatggaa gtataatttg gattttggag
     1561 agaagtaagt ctataaatga taataaagga agtgagtgta ttcagggagt tttaactgat
     1621 ataacagaat ttaagaaaat acaagaagag ttagattcaa aaagatttga aatacaggct
     1681 atcaatgata gtattgttgg tggaatagtg ataactgaga taaatgatga ttttgatagt
     1741 atatatttaa atgaaggtta tttaaatatg attggatata cacgcaaaca actgaaagat
     1801 gagctaaata ataaattaat caaacttatt tttaaagatg atagaacaga attaattaat
     1861 agtataaaca aacaactcaa attaagtgat acatttgcat ctgaacaaag agttgaaaaa
     1921 agggatggta ctattatatg gattttatta aaaggaagaa agacaattta tgataatgga
     1981 aaatctgtac tttgttgtgt tattgtagat attacttctg ctaaattatt agagcaagaa
     2041 cttaaaaaga gtgaagaacg tcatgaaatc attctaaagc agacgcaaaa tattataata
     2101 gaatgggata tagaaaaaga taaaatgaat atatcaccta attggtataa taagtttaaa
     2161 tatgacccta taactgaaaa cgcaacaaaa aatataatta aatcagtaga tatatatcat
     2221 gatgatatgg ataagtttat ttcattaatt aaacttactt cacaaggtag tgagtatgaa
     2281 gagtgtgaaa ttagacttag aactagagat actatttacg cttggtttag tataaaggca
     2341 acaactttat ttgataataa tggtaaacct tataaagtag taggaagttt gactgatatt
     2401 gatgaatcaa aacgagaagc tgaaaagttg aaagaaaggg cagaaaaaga ccctcttaca
     2461 gatttgtata ataaaaaagc atcacaatca cttatagaag agtacataac attggataaa
     2521 aaaagaaaag gtgctttaat gattattgat attgatgact ttaaaggaat aaatgataat
     2581 ttaggacatt tatatgggga tgctgtatta agtgaagttg caaatgacct aataaatcta
     2641 tttagagcaa ccgatatagt aggcagaatt ggtggagatg agtttattgt atttataaaa
     2701 gatatttcta aaacaagaga tattataaat aaagcagaag aattggtaaa aacatttgaa
     2761 aggtcttttt taggtaaagg acgaaattat aaaatatcat taagtgtagg aattgctaag
     2821 tttccacaag atggtatgga ctatatgaca ctatttagaa atgcggatat ggctctttac
     2881 tcagccaaag ggaaaggtaa gaattgttat acattgtatg ataaaaaaat tgataaagtc
     2941 caatatataa ctaatacaag aagtgcagga gatagtgata atgtagagat gtcttttaaa
     3001 gaacatatca caaattatat atttgatata ctatatgaat caagtgatat agaattggca
     3061 gtaaatttaa ttctaagtat tgtaggtaaa cactttaatg tgagtcgcat gtatatattt
     3121 gaaaattcag atgataatcg agtatgtaaa aatacatttg aatggtgtaa cgaaggtata
     3181 acctcggaaa ttaataatct taaaaatatt tgttacaagg atttaggcaa ctatctttta
     3241 aattttaatg cagatgggtt attttattgt aatgatattg aaaatttagg tagtgatgtg
     3301 aaaaaagtac ttaagccaca aggcatacgt tcaatattac aatgtaaaat tttagagggc
     3361 aatgaattta gagggttcat aggatttgat gaatgttcat ataatcgtat gtggactaat
     3421 gatgaagtga acactttgac atttatatct aaaacgctca gtatgttttt attaaaaatg
     3481 cgtattcaga gtaagctaga agagtcttta acaattcaaa aatctgtgat ggataatatg
     3541 gatatatggg catatgttgt tgacaggaat acctatgaat tattatttat caataaaaaa
     3601 atgcttgagt cagcaccaaa cactcatgta ggtgatttat gttataaagc gatatggaat
     3661 aatcaagata aaccttgtaa aaaatgccct atgatatatt taaaagatgg tgatgatact
     3721 tataaatcat ttatgcgtaa taaaaagttt gaggtacagg caaatataac tacaactact
     3781 ttgaaatggt ttgatggtaa agatgcatgt ttaatatttg gaagtgaaat aactagtatt
     3841 gaagctgaag aaaatactta aaatactgtg ttatatatat acttaaaatc gcaatttgaa
     3901 tttattagac ttaaaatgtc tatataaatc atttaagttg tggttttaag tattttttat
     3961 gatataatta agcgttaaga acaaaattaa aagatacttt tgtataaaaa aatatttgtt
     4021 aaatatatat tgagcaggtg ataatatgga aaataagttt ttaccaataa gcaaacaaga
     4081 tatgatagat agaggatggg aagaattaga ttttgttcta gttacaggag atgcttatgt
     4141 agaccatcat agttttggaa cagcaataat atctagagtt ttagaaaatg ctgggtataa
     4201 agttggaata atagctcaac cagactggag aactacagat gattttatga agctaggaaa
     4261 acctagactt gcatttctag ttaatgcagg aaatatggat tcaatggtca atcattattc
     4321 tgtaagtaaa aagcatagag acaaggatat gtattctcca ggtgggaaaa tgggttatag
     4381 accagataga gctactatag tatattgcaa taaaataaga gaagcataca gtgatgttgc
     4441 aatagtaata ggtggaattg aagcgagttt aagaagattt gcacactatg attattggag
     4501 tgataaggtt aaaaagtcta tgttaataga cagtggagca gatttactag tttatggcat
     4561 gagtgaaaag cagattgtag aagtagccaa tgcacttaat gatggatatg acccaaaata
     4621 tattagacat attgatggga catgctatat ttcagataca ttggaagaaa tatatgataa
     4681 atatatatta attccatcat ataaagaaat ttgtgaagat aaaatgaagt atgtagaagc
     4741 ttttaaaatt caatatgatg agcaagaccc atttagaggc aatataattg ttcaacctca
     4801 tggaagtaag tatctggttc aaaataagcc agaaaaacca cttagtagag aagaactaga
     4861 tgaagtatat ggattaccat atcagaagac ataccatcct gtgtatgaaa aatttggagg
     4921 aataccagct atagaagaag taaaatttag tatagtaagt tcaagaggct gttttggtag
     4981 ctgttcattt tgtgcaataa catttcatca aggtagagca gtgcaaagta gaagtgaaaa
     5041 atcaattatt gatgaagcaa taggaataac aaatttagat gattttaaag ggtatataca
     5101 tgatgttgga ggtccaacag ctaattttag aagaccagca tgtaaaaaac aaattactaa
     5161 aggtgcttgt aaaaatagac aatgcttatc tccatcacca tgtaaaaatt tagatgcaga
     5221 ccatagtgaa tacctacact tacttagagc tgtaagaaaa ttacctaaaa taaaaaaggt
     5281 gtttgtgcgc tctggaatta gatacgatta tgtaatggca gataagaata ataaatttct
     5341 tagagagttg atagagcatc atgtaagtgg tcagttaaag gtagctccag aacatatatc
     5401 agaagaagtt ttaaagtata tgcaaaaacc agcaggaaaa acttatgata aatttagaca
     5461 gaaatttttt gctataaatg aagaattagg taaaaagcaa tatttgatac cttatttaat
     5521 gtctagtcac cctggttcta ccttaaatag tgccatagag ttagcagagt atttaagaga
     5581 tactcactac caaccagagc aggttcagga tttttatcca actccaggga ctctatctac
     5641 aaccatgttt tatacaggta ttgacccgct tacaatgaag ccagtttatg ttcctaaatc
     5701 aaaacgtgac aaggcaatgc aaagggcttt attacaatat agagctccta gaaattatga
     5761 tttggtatac tctgcattag ttgaagcagg aagagaagac ttaattggct ttggacatag
     5821 atgccttata aagccaaaaa atgaaaagcc ttattttaat agaaataatt ctaaaaaaaa
     5881 tgtatctaaa ggaacaaata aaaacaaaaa aactaataca aataatagaa gtaataaaaa
     5941 tcaacaaaaa agttcaacta aaaaaaagaa aaaacattaa agtataaata aaaatactta
     6001 aaaaagaagg attattcgat aaattaacag aaattagtat aaataaatat aattatttta
     6061 atgacgttaa ctttgggagg aagagatgaa aaagtatatt tctataatat tattagtggt
     6121 ggtgactatg gttgttttgg taggatgttc accaggaaaa gataactcta aaaataagga
     6181 gctatcagtt gtcaaacaag tgaaagtagc tgttccagat ggactaccag cagtggcaat
     6241 agctaaactt gcaaatgaaa atccagagat taaagatgga tatgaaacag tgtatagcat
     6301 tgaaaagaca cctgaggcga tttccactag agtgatgaaa aaagatgcag atatagctat
     6361 agttccttca aacatggctg caatagcgta taataaaact tcatcatata atattgttgg
     6421 aactataggg atgggttcac tttatttggt gtcaacagaa aatataaaag attactctga
     6481 tttagttggt aaggaagttg gatgtactgg aaaaggactt actccagaca taactataaa
     6541 aagtcttttg ttacaaaagg atataaatta ttcagatata aaatttaatt atgtaaattc
     6601 agcaagtgaa ctggtaccat tattggctac tggtaaagtt aaaacaggta ttgttccaga
     6661 acctgcactt agtgctttga tgtctaaaaa tccagatata aaaattataa aaagtttaaa
     6721 tgactcatgg aaggaagtaa gtggttcaaa agacggttat cctcagtcta ctttaattgt
     6781 aaagtctgaa tttttaagag ataataaaga ttttgttgat tcttttgtag gtcaattatc
     6841 aaatagtata gattgggcaa acaaaaatcc tgaagaatta ggtgcatatt ctgaaaaaat
     6901 aaaaatatct actgaatcaa agataatagg aaaatctctt gaaagagcca atttaaaata
     6961 tattccagtt aaggatatga ttaaagatta taaaaattac tatgagaagt tagctaattt
     7021 tgatgataag acattaggag gcaaagtacc agatgaagca atatactttg ttgagaaata
     7081 aaaaaaataa agaacaattt atagataaaa tggaggtaac actttcttgt gttatcctct
     7141 tatttttatg gcaaattatt gctttaaaaa ttgataatga tattttttta ccaacagtag
     7201 gtcaagtatt taatagtata aaagagatta tgctaaattc tagtttctat atagacatac
     7261 tttactctat ggggagatgt ttttttagtt ttttactggc tatgattttt gctattatac
     7321 tttcaatagt gtcttattta aatagatttt ttagaaactt attaaaacca atcaatgctt
     7381 tgacacgttc aataccaact atgatattgg tagtacttgc acttatttgg tttgaaaagg
     7441 atagtacccc ttttatagtt ggatttacta tagtttttcc tatattgtat gataacgttt
     7501 tgggggctat attgaatata gacaagaact tattagaaat ggctaatgtg tataaaataa
     7561 ggtttataga taaggtgtta aaaatttatt taccagccat aaaatttcaa attatatcta
     7621 tactagtatc aacattctct ttaagtttga aagttgctat agcaggagag gtatatagtc
     7681 aacctacata tggtattgga actatgatac agactgaaaa aattaacttt aatacatctg
     7741 gaatatttgc atggataatt attgttcttt taatttctgc tattttgcaa atagctcaaa
     7801 agtttttagc aaggagagct tttttatgga aaagataagg ttgagtattg aaaatataaa
     7861 taaaagatat gatagtcgta ttatatttag agattttaat attgattttt atgtaaatga
     7921 ggtaaattgt atccttggaa aatctggttg tggaaaaact acactcttga acataataag
     7981 tgggattatt aaaaatgaca ccaacaatct aaatatcaaa gaaaatttaa atagagtagg
     8041 aaataagctt gaagctagtt acatatttca agatgataga cttatagact ggttaacagt
     8101 agaagaaaat ataaagatag ttgtaaataa atactataat aaaactcaat taaataaaat
     8161 ttgtgatgaa tacttggaat tggttggaat tagtgattat aaaaaattct atcctcagat
     8221 gcttagtgga ggtattagac aaagagtaaa tattgctaga gcttttatat atccatcaaa
     8281 aaacattata atggatgaac cttttaaatc tattgatgca aagaatacac agcttataat
     8341 ggataatttc agaaatatat taagaaaaga aaaaagaact gtgttatttg taactcataa
     8401 tattgaggaa gcattgtttt tagcagatag gatatttatt ttaggagact ctccaattag
     8461 aataaaaaaa atattaaaaa attctaagga attggaaaaa aatgaagttt tgaagttgat
     8521 atagagttat aataatactg tcgttttaaa atatatttgg ttaaaatcta taagactata
     8581 ttaaaatagt acagaatatt caaaactgtg tataatgttc gtattttata aaaaggaagt
     8641 gaatgtatgg ttaagactaa tgctatgaga atattagatt caaataagat tgattataaa
     8701 gtaatgtctt atgaagtgaa aagtgaacat gtggatggtg ttgaggtggc acatgatata
     8761 ggaagagatg taaatgaagt gtataagaca ttagtaactc aaggagttag taagaatata
     8821 tatgtatatg taataccagt acatgaaaac ttagacttaa aaaaggctgc aaaagtagct
     8881 aaagagaaaa gtgtagagat gattcatgta aaagatataa ataagcttac aggttatatt
     8941 agaggcggat gttctcctat aggtatgaaa aagctttata aaacttttgt aaatgaaagt
     9001 gcaaagaatt tagatacaat tattgtgagt gcaggaaaaa taggatatca aatagaacta
     9061 tctccatttg atttacaaag acttataaaa gtagaatttg tagatgtaat aaaaaaataa
     9121 gaattacata taaaatatag agcttatatt ctcaataata ggtgttgtaa tattaataaa
     9181 atgaataata attttaaaat aaatattcag ttacttatgt gtaattggta taaaaatatt
     9241 gagaaatttt aaaaacaatg gtaaagtgta agtataatca cgaatatttt ttataaagta
     9301 atggagggat gttcgcttga gaaaaataaa atctgaacaa atcgtcgagc aggtaaaaaa
     9361 actttgtata gaagcgagcc tatatttagg agaagatgtt ttaagttgta taaaagaaaa
     9421 agcgaaaagc gaaaagagtg aagttggaaa aaatatattg aatatattag ttgaaaatgc
     9481 tgaaatagct aaagaaaaaa atataccaat atgccaagat actggtatgg ctgtattttt
     9541 tgttgaaata ggacaagaag ttttaattga aggagacaca cttactgatg ctataaatga
     9601 gggagtaaga caagggtatg aagaaggcta cttaagaaaa tctgttgtaa gtccaattaa
     9661 cagagtaaat acaaaagata atacacctgc tgttattcat tatgatatgg taaaaggtga
     9721 taaaattaaa atagaatttg cagctaaggg atttggaagc gaaaatatga gtaagatgaa
     9781 aatgttaaag ccatcagatg gattggaagg aattaaaaaa ttcataatag atacagtttc
     9841 agaagctgga cctaacccat gccctcctat ggtaattgga gttggtatag gtgggactgt
     9901 ggacaaatgt gctcaaatag ctaaaaaggc actttttaga gaactaggtg aatttaataa
     9961 ggacgaaaat atagcaaaac ttgaaagtga actattaaca gctattaata aacttggtat
    10021 aggacctcaa ggcttaggtg gtactacaac agctttggga ttaaacatag agacttttcc
    10081 tacacacata gcaggtttac ctgttgttgt aaatataaac tgccatgcat caagacataa
    10141 gaaggtagta atttaggagg aaaataaaat gataaaaata actacacctg taaatgaaat
    10201 tgatatagca aaattaaatt gtggagatac aattagtttg agtggtatac tttatacagc
    10261 aagagatgct gctcataaaa gattgataga ttgtataaat aaaggtgaag aattaccatt
    10321 tgacgtatat ggtcaaggaa tatattatgt aggacctact cctacaaagc caggagaagt
    10381 tataggtgct gcaggtccaa cgacgagtta tagaatggat gatttgacta tacctctttt
    10441 agaaagaggt cttagattga tgataggcaa gggaaaaaga agtgatgaag ttatagaagg
    10501 tatgcaaaaa tacggagcag tttatcttgc tgcaatagga ggagctggag cgtatatttc
    10561 aaattcaata aaatcatgtg aaattattgc atatgaagat ttaggagctg aagctataag
    10621 aaagattgta gtagaagacc ttaagcttac agtagcaata gatagctatg gaaataatat
    10681 atatgagcaa ggtcgagcaa tttatgaatg taaataatgt ttagaaataa atatgaatat
    10741 caaaattaaa aatagttgtg tatattttaa ggaggaaaat tgatgtctaa ggattatgca
    10801 aaattggcat tagaaatgca tgaaatcaac aaagggaaag taagtgtaga aagcaaggta
    10861 gaaattaaaa ctaaggatga tttatcaaca gcatatacac caggggttgc tgaaccttgt
    10921 ttaaaaatac atgaaaatca agatgatgtt tatagataca cttctaaagg gaatctagta
    10981 gcagtagtta gtgatggaag tgctgtactt ggattaggta acattggtgc tgaagcatct
    11041 atacctgtta tggaaggtaa agctatatta tttaaacaat ttgcagatgt agacgctttt
    11101 ccaatatgtt taaagactaa tgatgtagat gagatagtta aatctgtaga gttgatggag
    11161 cctgtatttg gaggtataaa tttggaagac atatcttctc caagatgttt tgagatagaa
    11221 gagagattaa aaaaatcttt aagtatacct gtttttcatg atgaccaaca tgggacagca
    11281 ataatagttg cagcagccat aataaactca ataaagttaa ttgaaaataa aaaaatagaa
    11341 gatttagaaa tagttataaa cggagcagga gctgctggta tagccatagc aaagatacta
    11401 cttaatatga atgttaaaaa tataatctta tgtgatagaa gtggagcatt ggaagctagt
    11461 atagaaaatc taaactatgt tcaaaaagaa atgttaaaag taacaaatat aagaaatgaa
    11521 aaaggatctt taaaagatat tataaaagga aaagatgtat ttattggtgt ttcaggtcca
    11581 ggagctgtta caaaggaaat ggttgaaagt atgtctgaga agccaataat attggcaatg
    11641 gcaaatccta ctccagagat aatgccagaa gaagcaaaat taggtggagc atttataatg
    11701 ggaacaggtc gttcagactt ccctaatcaa gtcaataatg tactggcttt tcctggaata
    11761 tttagaggtg cacttgatgt taaggctaaa gagataaatg aagaaatgaa gatagctgct
    11821 gcatatgcaa ttgctaatac aataagtgat gaagaaatat gcccagagta tatattacca
    11881 gatgtattta ataagaatgt agtaaaaaat gttgctaagg cagttaaaga agcagcgata
    11941 aaaaccaaag taaatagaat ataaattttt atatgttcat atatccttat attatcgggt
    12001 atatgggcat ttttattata ggtttatact catatatcct tacttttata tacatatctt
    12061 tatgattaaa ggtgttagaa aatgtagtaa attttatact atcagactct atctttatta
    12121 ctttagtaga tacatggttg taaaggttta tagaaaaaaa tataggaaga cagattttta
    12181 ttgttgcgta tatatgagaa tgatataata tgaatattat aaattaataa atttaaattt
    12241 atggaggtac aagagatgat ggaatataaa gttaatgacc aagaacttaa tgcttcaatg
    12301 tttatcccat tcgtcaacag gatatggcca ggagattatg atatggataa aacacagact
    12361 gcattatcaa aaactttgaa tataactgct tatgatgata aagtgcttgt aggatgtctg
    12421 cgagttcttt cagatggtta ttattttgga acaattacag aattgcttgt tcttccagag
    12481 tatcagaaac aaggcattgg tagtaagctt ttccagcttg ctaaagagaa caccccaact
    12541 atgctatatt ttggtgcaca gccaggagta gaagagttct atgaaaaaaa tgggtgccaa
    12601 agaagtctgc aatcctacat aataaaaaag aaagatagtt attaaagttc cagctttcaa
    12661 taatcttaaa agtacaatat tttagtggaa tcatttcatt ttttattact aatatgacat
    12721 aaacatgtca tattagtatt gatatacttt taatatggtt aagagccaaa gaaaaatata
    12781 aggagtgatt taaaatggaa gaaactaaat tttgccaatg ctgtgcaatg ccaatgggac
    12841 aaactgatga attatatggg acaaataaag atggaagtaa aagtactgat tattgtagtt
    12901 attgttttaa agatggtgag tttacagctg atataagtat ggaagagatg atagaagttt
    12961 gtatccctca tatgcttcaa agtaataagg atatgactga agatgaagca agaacaatga
    13021 tgaacaattt ctttccaaca ttaaaacgct ggaaataatt ttatgcaaaa ttagttatat
    13081 gcaaaacatg aaatatggtg ttgagaataa ggggtggtgt gctttatagc ataccacctg
    13141 ttttgttact tggagttcag ttattttcct aaatatttta aatatactat tttagataag
    13201 ttccctcttc atcttgataa attaatcctt ttttcataag cactccaaga gctcgtttaa
    13261 aatttttctt acttgaatta aataatatag aaatatcttt agggtcagat ttatcattaa
    13321 atctcatata tccatcagaa ccttctaaat aacttaaaat cttattttct aaagtatcaa
    13381 gttcatcctt tctatttcct ctaggagaaa gaccaagttt tccatcttca tatattttta
    13441 tgacatgtaa attttcaaga acatcaccag cttttagttc agtaaagtac tcattatgaa
    13501 gtattacacc agcatattta ttgtctacac atatcattgc agaattatta gtttggaatc
    13561 catatacaac tccagttaca atatctccta cattgtaagt atggtctgtt aataaatatg
    13621 aatctatatc tgtagtagca gcaattcttt tgcttttgtc taaatatata taaaataaat
    13681 atttttcgtc tctaaatagt ggataagtct tagctttaaa aggaactaat atgtctttag
    13741 gaagtcccat atctataaaa gtaccaatgt ctgtattatc aactactttt aaatgtgcta
    13801 catccccaac cttagctaaa ggcggtatta aagttgcagc agttctgtca tcagaatctt
    13861 taaatatgaa agcatttact tcatcaccta tgtttatttc attttttccg attaatctat
    13921 tatgaagaag tatatcgtct tttgtgttat tagtttgtcc atccaaaaaa tatccaaatt
    13981 cagtttttct ttttactgta agtttattaa aatcacctat gtttatcaaa ttaaaatcat
    14041 cctctcattt tatatttata atttagccat aatatactta tgtagtagtt tgtcacagta
    14101 tctatattag tattagtgaa atttacaata aataatatag ttatttatgt atttttcaat
    14161 tctctagctt cttatatagt ataaactcca atttcttttt tgtctttagg ttttattaaa
    14221 atttttacaa aataagttaa aaagtattga aataaaattt cttttaaagt ataattggta
    14281 tataccaaaa ttggtatata ccatatcgaa aagtggtgac tatatgaaaa tagtaaataa
    14341 atctagtaat ataccattgc atactcaatt aagttcaata ataagagaga tgatagaaac
    14401 tggtgagctt aaagagggcg atgcaataat gccagaaaga gaactttgta acatacaaaa
    14461 tgtaagtcgt atgacagtaa ataaaaccat tgttggatta gttacagaag gacttcttta
    14521 tagggtacaa ggtaaaggga cattcgttgc aaagcaaaaa aagaagtatc agttttcaaa
    14581 tgtaaaaggt tttactgatg ttatgaaaga aaagggagta aacataaaaa cggatatatt
    14641 atcctttgag atggaactac cagatgattt agttaaaaga aagcttggaa taagtgataa
    14701 tactacaaat atatataaga ttgtcagact tagatatact gatggagagc catttggatt
    14761 agaaatagta tatctatcag aagaaatgtg taagggtcta acaaaaggga tattggataa
    14821 ttcttcatta tatagggttt taaatgaaaa atatggctat aagatacaaa aagctgaaca
    14881 ggtaatggag ccagtaatac tttcagatga agaaagtaag cttcttgaaa cagatgaagg
    14941 tgctttagct cttaaacttc atagaaattc ctataataga gaaggtagcc ctatagaata
    15001 taccatatca atatttagaa cagataaata tcaatatgaa atagtattga gtgagtaggt
    15061 gaataatatg aaatgtttaa ttaatggaaa gataatatta aaaaatcaaa tcttagaaaa
    15121 taaagtatta gtatttgatg aaaaaataat tgatatagct gatagtgtac caaaagattg
    15181 tgaagttata gatgctgatg gaaaatatat atctccaggg cttattgata ttcatataca
    15241 tggaaacatg ggaaaagata caatggattc gactgatgaa tctattgaaa caatttcaaa
    15301 atcaattatg agacatggag taacatcttt tcttccaact acaatgacta tggataaaga
    15361 acatgtttat gatgcacttg aagttattaa aaaagcccaa aatagaaagc ttgaaggggc
    15421 acaagtatta ggagcacatt tagaaggacc ttttataaat gagaattata agggagcaca
    15481 aaatgaaaaa tttataataa attcaaaata cgaatttata aaagaatata aagatgttat
    15541 aaaagttata acttatgctc cagaaaaaga tattgatttt gattttacta gagaaataaa
    15601 aagatgtaca gatatagttt tatctattgg tcatagtaat gcaaattatg accaagctaa
    15661 agaagctata aatttaggag taactaatgt aactcacatg ttcaatgcaa tgactggttt
    15721 aaaccataga gacccaggcg ttgttggtgc agcacttaca actaatgtgt acagtgagtt
    15781 aatagcagat acaatccata taaataaaga tttatttcaa tttatactaa ataataaagg
    15841 aaaagaaaga ctgattttaa taacagattc tatagaagct ggtggtcttg aagatggcaa
    15901 ttattctctt ggaggtcaga aagtcatagt taagggtaat gaagctagac ttgaaaatgg
    15961 agcattagct ggaagtgtgt tgtctttaaa taaaatggta tttaacttct tagataatac
    16021 aaaccttaaa gtgaatgaag caattaattt agcttcttta aatccagcaa catctttggg
    16081 aataaatgac aaaaaaggaa gcttagaaat aggtaaggat gcagatatag ctgtatttga
    16141 tgagaattta gattgtaaaa tgacactttg cttgggggaa gtagtatata aaaatatata
    16201 gtatacaaaa caaaccttaa agacagtaaa aacaatataa aaaataataa attaaactta
    16261 cattagtaaa agtagtagtt aaaatttagg aggaataaga tgagagtatt agtgtgtaaa
    16321 gattatgatg gaatgagtaa aaaagccgct gaaatgatag cagctcaaat agttttaaaa
    16381 ccaaattcaa tactgggact agctacagga agtacaccag taggtatgta tagagattta
    16441 gtaaaaaaat acaatgataa tatagtagat ttttcagatg tgatgagctt taatttagat
    16501 gaatattata agttaccaat atctaatgac caaagctatg attattttat gaaagagaat
    16561 ctatttaatc atgtaaacat aaagccagaa aatactcatc taccaaatgg aatggctgat
    16621 gatattgaaa aagagtgtat gaactatgaa gcttctatag atgcagctgg tggtatagat
    16681 gtacaagtac ttggaatagg tcgtaatgct catataggtt ttaatgaacc tgatactaaa
    16741 tttgcaaaaa gaactcatgt agttgaactt acagagtcta caatagaagc taatgcaaga
    16801 ttctttaaat caagagaaga tgttcctaaa aaagctgtaa gtatgggaat aggttcaata
    16861 ttgaagtcta agaaaatatt gcttttagct tcaggagaag aaaaagctga tgcagtatat
    16921 aatactgttt atggagatat aactccagaa gttcctggtt ctatacttca acttcataaa
    16981 gacactatag ttatagtaga tgaagcagca gcaagtaaat tgaatccaaa agattataaa
    17041 ttagtataag aatagaatgt agtttaagta aaaaatagct atcattgagt aatcaaattt
    17101 atttaatgat agctattttt atttaaattt tttacttctt tttatttaaa ttagttatta
    17161 ttgtttaaat tacttgctta ttacttttta cgaaaactcg ttatcaagaa gtatccaaat
    17221 aagatgttaa aaattattat gtttagtggg attctatcaa gcataataaa attacctata
    17281 attgggatat taccaactgg aaacataaag aataatacag gcatcagtga tagtaaaaaa
    17341 gatggaaaac aaagaagaag taatcttgga atatcaatat accaactacc atcctttttg
    17401 tattctctaa tcaataattc agttcctagt aataaaccaa taccaccaga ggaaattaaa
    17461 ttaataaaaa tgtatctttt aaagtcttgc atataaccag tgtgaaattg agtatagaaa
    17521 tcataattta atttaacggc taaaaaatac ataagcaata gaatgattaa aaataaaaga
    17581 tatctaagag cttttttcat tttaaattct cccttcagaa atactttatt cagttttatt
    17641 ttgttggaat ctgattttat catttagttt aagaaatcca taatattagt gaagaatgaa
    17701 ataagtatta tatatgaata aatattttta gaattaaggt gaattgtagt gcttaatata
    17761 actataatat taattgtttg aatattcaat aaaaagtttg aaaaatatca attttgatat
    17821 aaactgaata aatacagttg aatattaaat tattatttta tcaaaaatat gcaaagtact
    17881 acaccttcaa atatcaccta aagtaattaa aaggcaatta aagtgaagta gttcaagtat
    17941 ttctaaaata agtttaataa attctataat aatttatttt ttgccttaaa attgccttaa
    18001 aatctaaaca attttaaaac taatgtacta taaaaagctg gtataattta aatatagtaa
    18061 aaaataagat ttatcttatc tattgggatt taattattta atcaatatat ttataccatt
    18121 taatttaaat aaaaagtcta ttaaggagtg agcttatgag taagttaatt tatatagcag
    18181 atgatgaaga taatataaga aatttggtaa agacattctt aaaaaatgaa ggtcatgatg
    18241 ttatggattt taaaacgggt gatgaacttt tagaacaatt taatataaag gagtgtgact
    18301 tagtaatact agatataatg atgcctggct caagtggttt tgaagtatgt acaaaacttc
    18361 gtgagaaaag tacagtccca ataatcatgc ttactgcaag agatacagat atagattata
    18421 ttacagggat aaccttaggt agtgatgatt attttacaaa accatttagc ccaatgtctt
    18481 tggttatgag agtaaaatct atatttagaa ggatagagtt tgaaaagaaa caaaattatg
    18541 ataaatatag taactcgata gatatggaat taaaatttgg tgatgtaata ataaataaaa
    18601 agaataaaat cgttacaagt aaaaatgtaa atatagacct tacaccaaat gaatataacc
    18661 tattgactta tttgtttgaa aatatagata gagcagtttc cagagatgaa ttattaaaca
    18721 aaatatgggg atacgatata gaagttgaga caagggctgc tgatgataca gtgaagagat
    18781 tacgtaaaaa aattttaaat acaaatattt taatagaaac tgtatggggg tttggattta
    18841 ggttgaagga gaaaagttaa gatgaagaat ggaaatgaca agaatagaat tggaaagatt
    18901 aaatccaaat ttaaattaaa gttctcaaag aaaaatataa gcataaaaac taggattttg
    18961 ttgagtatac tttgtttgat aattatagta tttatgataa tattagtatc ttttaatgta
    19021 tttcttgata catatataaa aacaactgcg aatgaagaat tgacaaagtc tacagaaata
    19081 gtagaacata tggatagtaa ttttagacct attaagccac cacaagatga tggaaagctt
    19141 cctcccaaag tattatctaa ttttatgaga aatgtacaag ataaagttag gatggcagaa
    19201 acacaatcag atgctgatgc tatggtagtt gattctaagt ataacctgat atttccaaca
    19261 agagaggatg atttcttaaa aaatgttgat gagatggagc tgattaggga gtgtattcaa
    19321 aatgaaaaat tagatttaaa ttcagatgca aatacacgga tatctaccag aaataaggat
    19381 tattatattt caactgtaaa aattactagt atatatggtg aacaagacaa gtatcttata
    19441 ctttttatag acatatcaaa aactctaaat ctagcacaaa agataaatat tgtattaata
    19501 tctgtaatgt gttttgctgg aatattagca atatttacag ctgtgatttt atctgagaaa
    19561 attgctaaac cgataaaaga gttgtgtgag tttgcaaaaa aaatgggtca aggagatttc
    19621 aaaagaacct actttgactt ttcagataaa gaattagtag agctctcaac tgttatgaat
    19681 aagagtgctg agtatctgga taagtatgat aatgaacaaa aaatattttt tcaaaatgca
    19741 tctcatgaat taaggacacc acttatgtct attaaaggtt atgcagaagc tataaaatat
    19801 aatgttatag atagtaaaca tgcaagtgat ataattcttg aagaaagtga taggttaagt
    19861 gatatggttg aagatttatt atacatttct aaaatagata atataactaa agattttgaa
    19921 cttgtagaat gtgatttaag agaagtactt tcaaattgtg gagctaggca aaatgtaaga
    19981 gctataaata aggggataaa atttatttat gaatttgatg aagaaatggt tttatttgaa
    20041 tgtgatgaaa aaaacatttc caaagcattt atgaatctta tagaaaatgc tttgagatac
    20101 gctaagagtg aaataaaaat agtgtgtaag tataatcaaa aaaatatagt tgtgataata
    20161 gaagatgatg gaattggaat caagaaagaa gatttacctc atgtttttga aagattctat
    20221 aagggagtag gaggaaatca tggaattgga ttatcaatag ttaaatctat tgtaaataag
    20281 catggtgggc gtatatacgt agaaaatggc aaaaaaggag ctaaatttac tatattattt
    20341 aaattgtaaa aaaattagag aaaatataga gatgtttgaa gttgtttttt atatatatct
    20401 gttgtatact gttgaaaaaa gaacaatatt aagctgacat atattacgat agttgacatt
    20461 taaagtaaat gcttaaatat agatacgaaa ggaaaagtaa aatctgaagt gtatttaggt
    20521 ttaagtaagc ttcttatttt ataaaaaata tgtatgaaag aacagctgat ttttaatatt
    20581 acaaatttag taacggttgt atttgaagct tttgtcatcc atatgttttt atcagacttt
    20641 ttaggtcaga aagaagggta tataaaagtt gtaagatatg ctaagctggg ttttataata
    20701 tgtttaggat tctgtaattt aataacttta aaccctaaaa tcacaatgcc tttaattttc
    20761 atccttattt tttcaacatc atttttatat aaaggaaatt taaagacacg attgtttact
    20821 actgttttat taagtatatt ctttatactg tcagaaattg tagttacaag tatttttgtt
    20881 ttatttgtaa aagaaggctt tgaaataatg cttgaaaata atagtataag agtattggcg
    20941 acaattttat ctaaaattgt ctttttgttg acttgtaaaa ttatttgtct atttaaaaag
    21001 gatgtacatt tagatatgcc cataaagtat tggctccctt tatttttaat acctatattt
    21061 agtttatttc tgtctgtttc tatctttgat gtaagtaaat tttttagtct tgaaagttta
    21121 aaatttctaa gcttaatatc tagtgttggt attttatata ttaattttat tgtcttttat
    21181 ttatttaaat ttattataga taaaactaaa ctatctatga aatatgaact acttgaaaaa
    21241 gaaataattc ataaggaaga attaagactt agtaatgagt gttataaaat tattgttgaa
    21301 cagactgatt ctgtcgtttt cgaatggaat atcaaagaaa ataaatcatt tgtatcgcag
    21361 gcttggactg aaaaatttgg atataacaat gcatgtaaga atatttttaa agaaattaaa
    21421 gataaagact tagttcattc tgaagataaa gctatatttg aaggttttct tgaaagtatt
    21481 aaaaaaaaga atatgcacaa tcaagcagta tatcgtttaa aaaaatctaa tggtgaatat
    21541 atttggtgta gaacatcaat tacaagcatt tataatgatg aaaatgaact tttaagagtt
    21601 gtcggagtaa ttgtagatgt agatagtgat ataaaaaaat atgaagagtt aagaactaga
    21661 gcagaaagtg actcattgac caatatatac aataaaggta catttgaaaa attagtggaa
    21721 gaaactatag ttatgaatac aggtgataaa aaagatgcat tattcattat tgatttggat
    21781 gattttaaag aaataaatga taattttggt catccatttg gtgattttgt actaaaaaca
    21841 tttgctgata agatacaaac tagttttggt agtaaagact tagtcggaag aattggtgga
    21901 gatgaatttg ttgtatatat gcaagattat gttacagaag taaatttgca taaaaaagct
    21961 aaagaattaa atagagtact ttcggataat tacactgatt tgagttttag ttttgatgct
    22021 tctgtcagta taggtattgc aaggtatcct caagatggaa cttcattttt tgaattgttt
    22081 aaaaatgcag atagagcact ttatagcata aaagcatcag gtaaaaattc atattgttta
    22141 tttgaagaag aactatatgt acagtgaatt tgaatttaga ttttatgtga gagtaagatt
    22201 tatatatact tgtggctgtc tgatttataa aatatgttac tttgtatatt tttatatgtt
    22261 tcagtaaggt taattaacat aagcaaaggt gtaagtaaat ttatattagt ttaagtttac
    22321 ttacaccttt ataaaaatac aaataataga ttcatttaca ttcttaaatg attaaagctc
    22381 tttaccttct aaacatgcat aacatatcgt cataattcta gtaaataaga ctaaaatgcc
    22441 cattcctaag attacaattt catcatctat tactccaaat gtttttaaag taacttattt
    22501 cgctttgtat cataataatt tgtctctttt tacattttaa cttctgctga gatttgagtt
    22561 agatattcat tagaatctcg cacacttata tcatataaaa gatattcttc ataaacaaaa
    22621 tcccccatac ataaattatt ctgattaaaa aaatttaata acttttcata agttttgtaa
    22681 atagtttcat aacttccttt atgatatgca gttacataaa gtccctttgg tttgattgat
    22741 ataggatatt tcttgtactc tttattaact tttgtaaata aatatgaata tgattcaaag
    22801 tctttattaa ttatatcttc acctttaatg attgagccaa tcgtaccata atcactcaac
    22861 tcatagttgt ccataaagtt aatacattca tataaaaaat tttcttcctc atctcctaaa
    22921 aactgttctt tatatataat aggcgttttg actaagtact cttcttcatg ttcttttaaa
    22981 ataatagtat tcgcatctat attacaggca tttttagtaa atgaaatagt agattccatt
    23041 agagcttgaa tattatttag tttttcaatt tcatttttaa tatcaatagt tttcttttct
    23101 aaaagctcaa ttagtaaatt aggtgttctt ttgtctatat aagttttaat ttcttttaaa
    23161 ggcattttta cttccctaag tgaagaaatt ataccaaaaa tgctaagttg atggtatgag
    23221 tagtatctat atccattttc tttctttaat tctggtgaga aaagacctat atcgtcataa
    23281 tggaaaagtg tttttttatt gattccacaa atctttgcaa attctccagt tgtaaaataa
    23341 ttttttgaaa cttgactcat aattataatt cctccttgac tatcctgtta cagtatagtt
    23401 tatcataata ttttgtaaag gagtgtgatt gtatgaaaaa attaattcat tttttaaaac
    23461 catatagagt tttaattgtt atcgttttga tatttacgtt tttacaaaca cttggaactt
    23521 tgtatatacc aacacttaca gcaaatatag taaacaatgg tgtagtaaag ggagatatag
    23581 attatattgt aaaaacaggt ttaatgatga tgatagtagc tggtataaca gctttatcag
    23641 ctgtgttagt ttgtaaagta tcagcaaatc tttcatctgg attttgtaga gatattagag
    23701 aagcggtatt tgtaaaatca caagatttat caataaatga ttttaataat ataggtacag
    23761 catcaatgat tactaggagt actagtgaca ttactttaat aggtcagtct gtgtttatgt
    23821 ttattcaatt agtattacca gcaccaatta taactgtttc tggtctattc ttagcatatt
    23881 ctatagataa agcaatgaca gttattatta ttgtagtaat gtttctattc atgttatcag
    23941 catttttagt tgggaaaaag cttatcaagt tgtttaaaat gatgcaagta aaaatggaca
    24001 atatgaatag ggtgttacgt gaggtagtaa ctggagtaag agttataaga gcttttaata
    24061 gaagtcattt tgagaaaaag agatttgata gaactgcaat agattattct gaaacagcaa
    24121 tttcaataaa taaaatattt gcagtactta tgcctattgt aatgttgata atgaatctag
    24181 gtatagtaag tattatttgg tttggcggga tgagagtcag taatggaaat atggaaatag
    24241 gtcatataat ggcacttgtt gaatattgta tcttaatttt attctattta atcatgggag
    24301 taatggtatt tatgtacata cctagagcag gtgcatgtgc agatagagta aatcagatat
    24361 tagatataga acctgaaatt atagatggaa atggtcataa agatactgtc tcagagaggg
    24421 ggcatttagt atttaaaaat gtaacatttt catatgcaca atcagaagaa cctgttttaa
    24481 ataatataac atttgaagca aagtctggag aagtaactgc aattatagga agcactggct
    24541 caggcaaatc tactattgca aatataatac cacgtttttt tgaaattcaa tctggagaaa
    24601 tatctataaa tggtcaggat attaagaaga ttcctcaaaa agaacttaga gataagattg
    24661 ggtttgtacc acaaaaagct tttttattta gtgggacaat agaagaaaat ataaggtatg
    24721 gaaaagaaga tgcaagtatt gaagaagtaa agtatgctgc aagtatagct caagctgatg
    24781 aatttatttc tgatatggaa gataagtatg actcatttgt agcacaaggt ggaaataatt
    24841 tgtcaggtgg tcaaaaacag agaatatcca ttgcaagagc attagttaga aagcctgaag
    24901 tatatgtctt tgatgatagt ttttctgcac tagattttaa gacagataaa agacttagaa
    24961 aagcgttgaa gaatgaaatc aaagattcat cagctataat tatagcacag cgtataagca
    25021 caatcatgga tgctaatcaa attatagttt tgaatgatgg aaaaattgtc ggtattggaa
    25081 aacataaaga tttacttgaa aattgtgagg tttataagca aattgcagat tcacaactta
    25141 gtaaagaaga gcttgcatag gatgtagaat taatctgtgt atttggaggt atgtaatatg
    25201 agtgaaaaaa gaacagataa acaagatata gaggtactta gttttgaaga tggtatgtca
    25261 gcagaaaaag ctaaggatgc aaaaaaaact actaagagat tattaaaata tatggcaaaa
    25321 caaaagttta aaatattaat tatttttatt tcagtattga taagttcagc acttacagtt
    25381 ttggcgccta tggttatggg aaaagctatt gaccaactat ttaatggaat aaaaactgca
    25441 gtccaaacag gtacaaaatt cagtgtgaat tttagtacta tgggaggtat agtatcaata
    25501 cttctagggt tgtacttaat tagtgcagta tttatatata ttcaacagtt cattatgtca
    25561 ggagttgcac aaaatctagt tcttagcatg agaaaagatt taagtgataa actcaataaa
    25621 cttcctctta agtactatga ttctcataaa aagggggaaa cacttagtat agtaacaaat
    25681 gatttagaaa aagtagcaga tagtttacaa gaaggattaa tgcaacttat tacagcagta
    25741 gttacagtag ttggttctat tgtaatgatg ataagtataa gtgtaccact tacaatagta
    25801 tcagctataa cattacttgt ttcattgggt attactgttg taattgcaag aaaatcacaa
    25861 gaaagattct ctgaaaatca aaaggcacta ggagagttaa atagcaatat agaagagata
    25921 tttactggtc aaatagtggt taaagcattt agtaaagaaa aggatactat aagaaacttt
    25981 aaaatattaa atcaaaatct ttataatgca agtagaagag cacagttttc gtcatatgca
    26041 atatctccaa ttattagatt tataaaccta attggttatg ttattatagc agttgtaggt
    26101 ggtatatttg catcaactgg agcaatgact cttggtagta tacaagcgtt tattcaatat
    26161 gtaaatcaag catcagagcc tactacagaa atatcttata ttgtaaatat gcttcaagca
    26221 gcaatagcat ctgcagagag agtttttaca gttatggacg aggtagaaga aataaaggat
    26281 gaagaatctt caaaggttat tagcatgcct aaaggtaaag tacaatttga gcatgttaaa
    26341 tttggatata gtgatgattt tattcttatg aaagatatta atatcaatct aaatgcagga
    26401 gataagattg caattgttgg acccacagga gctggaaaga caactcttgt aaatttactt
    26461 atgagatttt atgaaataca aggtggaaga attactattg atggtgttaa tataaaagat
    26521 ttaaagcgtg gagaacttag aactatgttt ggtatggttc ttcaagatac atggctgttt
    26581 aatggaagta taaaagaaaa tattgcatat agtaagttag atgccacaat ggatgaaatt
    26641 gtaagagcag caaaatcagc aagagtagac cattttataa ggactttacc acaagggtat
    26701 gacactatat tgactgagga tgcttctaat ctatctcaag gtcaaaagca acttctaaca
    26761 attgccaggg caatactttc agacccatca gtattgattc ttgatgaagc aacatctagt
    26821 gtagatacta gaacagaggt agaaattcaa aaggcaatga ataacttgat gaagggaaga
    26881 actagttttg ttattgcaca tagattatca actattcgag atgctgattt aatattagta
    26941 atgaaagaag gcactattat tgaacaagga agccataagg aacttattga aaagaaagga
    27001 ttctatgaag aactttataa cagccaattt tcaagtgaat atgatgaaga tgttgtatga
    27061 ttatcaagtt ttaggtaact aaatgactga taaatattaa gttaaatata gtaacatata
    27121 aggttatatg gaataagagt atttttctat ttcatataac cttttttatt tgtcaaaata
    27181 agataaaatt tcctttctaa attagattga agaatgtatt tatcaatata aaagtaaaat
    27241 aacccttatt attattgaag taaatattgt ttgccacata tatgccataa agatgcactt
    27301 attttacgga gatttaacaa agtagtgtaa agtatactct aaatataaaa taaatcaaat
    27361 agagttaaat aaattgagag gtgatataaa tagtgaataa aaaattgaat tatagatttg
    27421 aaatgaagca caaaataact gaagcagatg tgctagcttt aaaatcaaga ctctatccaa
    27481 caatgaagaa agatgaaaat gcatctaaag atggaaaata tcttattagg agtttatatt
    27541 ttgatactcc tgaagataaa gcacttttgg ataaattaaa tggcgttgca acaagagaaa
    27601 agtttaggat aagattttat aataatgact gctcttatat aagactagag aaaaaaatca
    27661 aacattacaa catgacatca aagctatcag ctagtttaac caaaaaagaa gtaataaata
    27721 tactaaacaa tgatattgaa tttctaaaag agtcgacaaa ttgtttatta agagaatttt
    27781 atttgaaatt aaaatgtgaa agattagagg caaaaagcat agttgattac aatagagaag
    27841 cttttattta tccagttgga aatgtaagag ttacaatcga tagtgatatt aaaacttctg
    27901 taaattcggt agatttattt aataaagatt tgccaactgt ttcggtaatt gatgagaata
    27961 tgactgtatt ggaggttaaa tacgatgaat ttataccaga ttttataaag gatttaattc
    28021 aggttaataa gactacttct acagcagttt caaagtatgc ttcaagtaga ttatatattt
    28081 aagaataaag gctgtgattt atataaagtt atacataaat aaggttaaaa aacaaataaa
    28141 ttaaaaataa ataagttaaa aataggaagg atgattttaa ttatgacatt taatgatatt
    28201 tttaaatcaa gttttataga aaacgtatca ggtttttcat ttgtggacag tgctttagcg
    28261 ctaggttcag cttttttagt aggtttattt atatatatgg tctataaaaa gacttatatg
    28321 ggaattatgt attctcgacc attcaatgtg tcgcttgtag cacttacaat gcttacaact
    28381 tttgtaatat tggcggttac ttcaaatgta gttttatctt taggtatggt tggtgcttta
    28441 tctatagtaa gatttagaac agcaattaaa gacccaatgg atttagtatt tctattttgg
    28501 tcattgggtt caggtatagt tttaggagca ggtttgattc ccttagctgt aatgggttca
    28561 ataattatgg gattgattct aatattcttt tcaaacaaaa caatatctga aactccatat
    28621 atattgatgg taaattgtaa taatgaagat tcagaagata tagcaactga taaaattaaa
    28681 aaagtattta ctaaatatca aataaaatca aaaagtgtta caccagaaaa aggaatagaa
    28741 ttagtgtttg aagtaagaat gaaagatgga gagacaagtc ttataaatga cttgagtcaa
    28801 gtagatggag ttactaatgc agttcttgta agttataatg gtgactatgt agcatagaga
    28861 ggagtaatct tttatgaaag ataaaaaatt tacccttctt atctcgatta tgattatatt
    28921 tttatgtgct gtagttggag tttatagtac atctagcaac aaaggtgttg atttatatag
    28981 tgatgtatat attgaaaaat attttaacag agacaaggtt atggaagtta atatagagat
    29041 agatgaaagt gacttgaagg atatgaatga aaatgctata aaagaagaat ttaaggttgc
    29101 aaaagtaact gtagatggag atacatatgg aaatgtaggt ataagaacta aaggaaattc
    29161 aagtcttaca tctgtagcaa atagtaatag tgatagatac agctataaga ttaattttga
    29221 caagtataat actagtcaaa gtatggaagg gcttactcaa ttaaatctta ataactgcta
    29281 ctctgaccca tcttatatga gagagttttt aacatatagt atttgtgagg aaatgggatt
    29341 agcgactcca gaatttgcat atgctaaagt ctctataaat ggcgaatatc atggtttgta
    29401 tttggcagta gaaggattaa aagaatctta tcttgaaaat aattttggta atgtaactgg
    29461 agacttatat aaatcagatg aaggaagctc gttgcaatat aaaggagatg acccagaaag
    29521 ttactcaaat ttaatcgttg aaagtgataa aaagacagct gattggtcta aagtcacaaa
    29581 actattaaaa tctttggata caggtgaaga tattgaaaaa tatcttgatg tagattctgt
    29641 ccttaaaaat atagcaataa acacagcttt attaaacctt gatagctatc aagggagttt
    29701 tgcccataac tattatttat atgagcaaga tggagtattt tctatgttac catgggattt
    29761 taatatgtca tttggtggat ttagtggttt tggtggaggt agtcaatcta tagcaattga
    29821 tgaacctacg acaggtaatt tagaagacag acctctcata tcctcgttat taaaaaatga
    29881 gacatacaaa acaaaatacc ataaatatct ggaagagata gtaacaaaat acctagattc
    29941 agactactta gagaatatga caacaaaatt gcatgacatg atagcatcat atgtaaaaga
    30001 agacccaaca gcattttata cttatgaaga atttgaaaaa aatataacat cttcaattga
    30061 agactctagt gataataagg gatttggtaa taaagatttt gacaacaata actctaataa
    30121 cagtgattct aataataatt ctaatagtga aaataagcgt tctggaaatc aaagtgatga
    30181 aaaagaagtt aatgctgaat taacatcaag catagtcaaa actaatacag ataatgaaac
    30241 taaaaataaa actacaaatg atagtgaaag taagaataat acagataaag ataaaagtgg
    30301 aaatgataat aatcaaaagt tagaaggtcc tatgggtaaa ggaggtaagt caataccagg
    30361 agttttggaa gttgcagaag atatgagtaa aactataaaa tctcaattga gtggagaaac
    30421 ttcttcgaca aagcaaaact ctggtgatga aaattcaagt ggaattaaag gtactgaaaa
    30481 gtttgatgag gatatgagtg gtatgccaga accacctgaa ggaatggatg gtaaaatgcc
    30541 accaggaatg ggtaatatgg ataagggaga tatgaatggt aaaaatggca atatgaatat
    30601 agatagaaat caaggtaacc ctggaggttt tggcaataga ggagtaggct ctataagtaa
    30661 aacaacagta tactccaaat taattttagg tggagcttca atgataataa tgtcgattat
    30721 gttagtaggt gtatcaaggg taaagagaag aagatttata aagtcaaaat aagattaaaa
    30781 ttgaatataa ataattttaa tactgttaag tctaaatatt tacttagcag tattttttta
    30841 gaaaataatg tatttagtta ctaatataaa tgaaattgaa aacatttgaa ggatagaagt
    30901 tggttggata aatgtataaa ttacttcacc aaaaaataat gacaaaaatt gtaactaagt
    30961 tgtaataaaa tggtagttat ttagcataaa ataatgtatc atagaagtgt attataaaat
    31021 taataaaata ggatttttat aagaaaactg ctggcttttg atatttaaag tgagtagtta
    31081 aattatgttt aggaggggta gaatttgttg caaaaagtat tatggaaatt tttaaagcta
    31141 gtagtcggtt tatttatatg ttcagttggt atagtattga cgataaattg taaccttgga
    31201 ttatctccct gggatgtatt tcatcaagga ctttcaaatc atataggaat aactatagga
    31261 actgcttcta taattgtagg ctctatagta gttattgctg atgtcgttct tggagaaaat
    31321 gtaggatggg gaactgtttt taacatgtta ctaattgggt tttttatgga tttaatttta
    31381 tatagtaatt taattccaga ggctgatagt ttatttgtag gaataattat gttgatatta
    31441 ggattggttc ttttgtctgt tggaatggta ttttatatgg gaagtggttt gggaagtgga
    31501 cctagagatg gtttgatggt tgcacttcaa aagaaaacag gaaaatcact taaattaata
    31561 agaggtacta tagaagttgg agcattaata gtaggatttt tattaggtgg aaaagttggt
    31621 ataggaacta taataagtgc atttggactg gggtatttta cacaaatggt atttggctta
    31681 tttaaattag attgtagtaa gattaaacat agatttatag tagatgatat aaaatttatc
    31741 aaagtgtata tgagtggtga gaaaaaagct gctgttcaaa atatagttaa gtaatttaag
    31801 aataaattca acgaaatgtt aagtatttta aattaacatt ttgtttagat ggataaattg
    31861 aatattaaaa gaacattaaa atatgaggat tgctagaaca ctataaaata tagatttcta
    31921 gcaatttttt atttgttcaa tgttttttta gtattagaag tcttattctt tatttaatga
    31981 atgaattaat ttataattag agatactaaa ctttatagat attaatataa acacctttaa
    32041 actgtgtatt ttccaaagcc tttaaaatgc ttgtatttat tctaaataaa aaaataaaaa
    32101 aatgtcaaaa aaactcttgt attatatcca aattttatat ataattacct atgttgttta
    32161 gaaattttaa actcaatgtt tagtaaacaa aatgtttagt aaaataaaac agaaactagg
    32221 aggtgcaaca tggatatagg tgaaaagata aaaagattaa gaacagaaaa acaactgacc
    32281 caggaagaat tagctaatag atgtgaattg tcaaagggat ttatttctca gttagaaaat
    32341 aacttaacat caccatccat agctaccctt atagatatac ttgaaatact aggtacaaat
    32401 ttaagggaat tttttaatga aatagatgat gaaagaatca gttttacaaa agaagatatg
    32461 tttgaaactg aagatgaaga tttaaaatat aaattaaagt ggctaattcc aaattcacaa
    32521 aaaaatgaaa tggagccaat aataataaca ttgtatccag gaggacagta taaagaagaa
    32581 aagccacatg aaggtgaaga atttggatat gtattagcag gttcaatata tgtccatata
    32641 ggcgaaaaga aaaataaagt aaaaaaagga gagagttttt actttagacc gaaagcaaat
    32701 cactatatat caaacgaggg caaaactaca gcaaaagtga tttgggtaag tacgccacca
    32761 tcattttaga atagaggtgt ttaataattg gtagaaaata taatagaatt aagggatata
    32821 tctaaacatt atgaagaact tactgtttta gataatttca atttagatat aaagaaaaat
    32881 gagtttctta cattacttgg accaagtgga tgtggaaaaa ctacaacatt aaaaataata
    32941 gcaggatttg agtatgctga tgatggtaaa gttttatttg aaggtaagga gataaataat
    33001 ttgccaccat atgaaagaca agtaaacaca gtatttcaaa aatatgcttt atttccacac
    33061 atggatgttt atgaaaatgt agcttttgga ttaaaaatta aaaagactcc aaaagatgta
    33121 atagatgcta aagtaaaaga ggttttgaaa cttgttgcac tagaaggttt tgaaagacga
    33181 caagttgaat cattgagtgg aggacaacaa caaagaattg caatagcaag agctctagtc
    33241 aatgaaccaa aggtattatt actagatgaa ccacttggag cacttgattt aaagttaaga
    33301 caagagatgc aaatagaatt aaagagaatc caaaaaaaat taggaataac atttgttttt
    33361 gtaactcatg accaagaaga ggctcttact atgtcagata ctattgttgt aatgtataag
    33421 ggcaaaattc aacaaatggg aacaccacaa gatatataca atgaacctaa aaattcattt
    33481 gtagctaagt ttataggtga aagtaacata tttgatggaa ttatgataga agattataaa
    33541 gttaatttct gtaatagaga ttttgagtgt gttgataaag gattcgaaaa aaacgaaaat
    33601 atagaagtag taataagacc agaagatata aaaatggtaa agccagaaga aggaatgtta
    33661 aaggggaaag ttacatctac agtgtttaaa ggagtgcatt acgagataga attgaatgaa
    33721 aatggaagat tgtggatact tcataatact aaaaatgctg aggttgggac tgaactaggc
    33781 atggatatat atccagaaga tatccatata atgagaaaag agagtaacta agatgaaaag
    33841 aaaatctttt ttagcatatc catatgtagt ttggagtgcg atatttgtaa taattccttt
    33901 aatactagtg gtatttttta gttttacaaa agaatcaggt ggaggttatg cctttacact
    33961 tgagaattat aaagaagtaa ttgaccctat atatatgaaa gtttttgggc gttctattct
    34021 tttagcagga ggagctacat taatatgcct tatagttggt tatccagtgg catatataat
    34081 ttcaaaggct cgtgtaagta gaagaggttc attaatatta ttatttattc ttcctatgtg
    34141 gatgaacttt cttttaagaa catatgcatg ggttgctata ttaggaaaaa atggactttt
    34201 aaatacattt ttaggttggt ttggaataca accattagca attttgtata ctaattttgc
    34261 tatactttta ggtatggtgt ataacttttt accatttatg gttcttccaa tatacacagc
    34321 actatcaaag atggataatg atttgataaa cgctgctcat gacttgggtg caaataatat
    34381 gacagttttt agaaaaatta tatttccttt gagtttgcca ggtgtaatgt ctggaataac
    34441 tatggtgttt atgccagcag tcactacttt tgcaatatca agactgttag gtggaggtaa
    34501 aataatgctg gttggagatt tgattgaaca acagtttact gttgttggag attggaactt
    34561 tggttcagct atatcgatat ttatgatgat agtaatatta atatctatgt ctataatgtc
    34621 aaaatttgga gatgaatctg acaaagaagg cggtgggtta ctattttaaa attaaagaaa
    34681 ataacttcta atatatattt agcattagta ttcttatttt tgtatgcacc catatttgct
    34741 ttggtactat tttctttcaa tgattcaaaa tcaatggcaa gatggaatgg atttacatgg
    34801 aagtggtatg gtcaattact tcaaaatgag agtataatga gtgctttata ttatactatt
    34861 gtaatagcta tattagcgtc tgtaatatct acaatagtag ggactataag tgctatagga
    34921 atacataaga tgagaggtaa gagtaaaaaa ttaatactaa atgtaaacta tcttcctatt
    34981 ttaaatacag agatagttac agcggtagct cttatgagtc tgtttgtatt tgtaaaaatg
    35041 gaatttggat ttacaacaat gttattggca catataatgt tttgtttgcc atacgttata
    35101 ctttcagttt tgccaaaaat gaagcaatta cctgataata tagaagatgc agctatggac
    35161 ttgggagcaa cacctatata tgctcttaga aaagtaattt taccacaaat aaaaccaggt
    35221 atagtttctg gatttttaat tgcatttact atgtcaatag atgactttat aataagtttc
    35281 tttaatgctg gaaatggtgt aagcaatctt tctatagaaa tctatggaat ggctagaaga
    35341 ggtataaaac ctgaaataaa tgcattatca acaataatgt ttgcagtggt tctaggttta
    35401 cttttactag ctaataaaaa agaatctata gtaaggggga ctaaataatg attaagttta
    35461 aaaaagtaat atcccttata gctgtaggca tgatgacagt tactttattt acaggatgta
    35521 ataaagcaac agattctaca aaagttttaa atgtatataa tgttggagat tatatagatg
    35581 agtctcttat agataagttt gaaaaagaaa caggaataga tgttcaatat tcaacttatg
    35641 atactaatga aatgatgtat caaaaagtga agagtggtag tacacactat gatttagtat
    35701 ttccttcaga ctatatggta gagaaaatga aaaatgaagg tcttttggaa aaattagatt
    35761 ttaagaatat acctaatatg aagtatttag ataagagttt tttaaatcct gtatacgaca
    35821 aaactaatga atatagtgtt gcatatatgt ggggaactct tggaatattg tacaataaga
    35881 aagaagttaa agaccctatg gatagctggg atatactttg gaatccaaag tataagggaa
    35941 atataatgat gtttgactct gttagagata ctataggaat aactcttaaa aaattaggat
    36001 atagcatgaa tagtgtaaat ccaaaggaaa taaatgaagc taaaaatctt ttaatgaaac
    36061 aaaaggattt agtactggct tatgtaaatg atgaaggtaa ggatagattg cttggtggag
    36121 aagtggcaat gggtatgctg tattctggag atgcagttac actaatggag caaaatcctg
    36181 accttgatta tgctatacct aaagagggaa ctaataaatg ggtagatgct atgtgtgtac
    36241 ctacaacagc gcaaaataag aaagaagctg agctgtttat aaacttttta ttagacccag
    36301 agaatgcaaa agtaaatgct gagtacattg ggtattcaac acctaataca ggagcactaa
    36361 aattattaga cccagaaata actcagaatc ctgttgcata tccatctaaa gaagtacttg
    36421 ataaatgtga gacttttgaa gatattggtg aaaatataaa attatatgac aaggcttgga
    36481 tagagttaaa atcaaaatag agtcttgttg gtttaagtaa atttacttaa taaatactta
    36541 ataaaactgt tcttttttag aaaatttatt ttctaattat tgaatagttt tattttttgt
    36601 ttaagttata tttttgttta ggtcatgttt ttattttgag tttattttta tgagaattta
    36661 aagtctagta agtttcattg attactattt ttataataat gactataaag aggctgtttc
    36721 aaaattgaga taagcttctt tttaattgtt gaaatgatga gaacatgttt gatttcatag
    36781 aaaaaagagt gattcatgag ctaaaaagtt cactttgaga cagtattttt tattaaatta
    36841 tattcatata caatttaaat agtttattaa taaatgaaaa cttatttgta taatgatttt
    36901 aataataatt agaaaaaatg ggcaacttaa tagaataatt ggaggtgtaa atttataaaa
    36961 aatgaatagg ataaataaaa aaatagattt attaatatta gctttgctcg tttttatatt
    37021 tttaattgtt ggaatagtaa tactatcgat taggacagaa aataaactga atgtgttcat
    37081 aatgctagca ataatttttt ttataataat gttaacttat ttatcaaatt cagtagtggg
    37141 attgataaca agttcaataa ttatatttat gtatacatcc tatattttat ataacaatat
    37201 tactcacaat atggatgttg aatttatatc atatatgtgg ataatagcaa taccaatatc
    37261 ttcaattata atgggtaatc tgaacaaaag tataaatgaa ttgcaaaata caaataaaaa
    37321 actatctgaa caatataagg aattagtgac tattgatagt gaaactggac ttagaaattt
    37381 aaagattttt tataatgatg taaatatgga gataagtaaa tctattagac ataatactga
    37441 tttttctctt atgatagtaa aattaccata ctacggaaat ctacagacta tatttggtga
    37501 aaacaaaact aataaaatag taaaatatat tggttcaaat attatagaat gcacaagaaa
    37561 tgaagatata atctatagtt tacaaaaaga tatgataggg atactaatgc caaatacatc
    37621 tttggaaggt tcaaaagttg ttaaagacag aataaagaaa aggataaaag aattaaattt
    37681 agatttaaac aataggggaa aatatgtaaa tatagatgtc aaaattgcat ttttacaata
    37741 taagagtagt tttggagata gtataaattt taagaatata gttgaagagg aacttcaata
    37801 tgatgtttaa aatattcaat aaaagtttat taaaattagt agttatatta tcaatagtac
    37861 tgacaatatc aaattttagc ttatcttcat atgcatcaga aattacttcc agcacagata
    37921 aggtattaat catatatgat tctaagaaag aaacagctta taatagagat attttaaata
    37981 ttatgaggac tctattggga agatttagtt cagatataga gctattaaaa ttgagtaact
    38041 atgatggtga gataaataaa aattattaca gtcatatatt tattttagga atcaatgaaa
    38101 actcatatga taatgataaa aacactaaaa acttgattag ttccttaaat tcatataaag
    38161 gaactatatg ttggttagga tatggtatag aaaatctttt ggaacataaa aaatacaatt
    38221 tagactatgt aggaaaaaca aacaatatag ttagtgtaaa ttatagaggt aaatcatata
    38281 acttggatga acattatgtt tttaacattg tagaatcaaa agatacaaat aataaagtaa
    38341 taggaagtat taatgatacc ttaaataagt atccatatat aataaatgat aaaaatcttt
    38401 tttatgtatc aaaattagat ttagatggag ttttatttta tatattttgc gattcactta
    38461 acgatatttt taatattaag acatttgata aaggacgtat atttgtaaga atagaagatg
    38521 tacatgcttt tagagaacca aaaaatcttg tagagatagc tgattattta agtagtaaga
    38581 atataccatt tacgattgca ttaataccag cgtatgtaaa tcctaaaaat cataaagtaa
    38641 taactttatc agaaagccct gaaattgtca aagcaataaa atatatgcaa gataaaggtg
    38701 gaactgtgat attgcatgga tatactcatc aatataagaa agaagaagtt tctggagaag
    38761 gatatgaatt ttgggatggt aaaaaagatg aacccttaaa agaaaatatg aagatatttg
    38821 tcaaagatag agttttgaat ggtcttagag tatgtataga gaatggaatt tatcctcttg
    38881 catttgaagc accacattat gcaatggaat cagatggata taaagaatta aaaaaatatt
    38941 tttctactta tatggggcaa catcaaaata atgataaaaa atttagcaca aatacatatc
    39001 cttatattat tagggataca gaagagttta atatatttat acctgaaaat ttaggatata
    39061 tagacccaga agataagttt acttttcaaa atataaaaga aaatttagat aaattatcca
    39121 ttgtaagagg ttttagtgga gggtttttct ttcattcata tttgaatata gagtatctaa
    39181 aaaatacgat agaatattta gaaaaacaga atatagaatt tatgaattta agagatttta
    39241 ataattgggt aaaagttgag gaaatacaaa taaaaaataa tggagatgaa attatagtta
    39301 actatgataa ggacttagat gaaataacta aaagtgatgc tagatttaaa tccatatcca
    39361 atattagtaa aattttaata ttcatagtat ctataagtgt tttaatattt gtgattatat
    39421 ttatatattt taagagaata gataaaaaga aatt
//
LOCUS       DAEORZ010000023        39056 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEORZ010000023 DAEORZ010000000
VERSION     DAEORZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39056
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.023"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(13..1893)
                     /locus_tag="KRH39_001042"
     CDS             complement(13..1893)
                     /locus_tag="KRH39_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBG8510962.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISIKKSGSNLNADYKVFGKGTSDKPALK
                     IVTYNNKTVDYENINSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGLNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDSETEQFIKSNKVSNIYFIGGI
                     EKISNKAIEQVGKIANRDISKNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2103..8066)
                     /locus_tag="KRH39_001043"
     CDS             complement(2103..8066)
                     /locus_tag="KRH39_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBG8510963.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGN
                     LEVDLNFSLPIKNSESESITNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVRKYNVEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDKISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPDGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQ
                     EINKSGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLKTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDVVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMASKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSSSFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKAKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDSNTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNAIDVSNKKVVTVSNKVKGNIVRDK"
     regulatory      complement(8190..8275)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8447..9850)
                     /locus_tag="KRH39_001044"
     CDS             complement(8447..9850)
                     /locus_tag="KRH39_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBG8510964.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMENKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(10303..12198)
                     /locus_tag="KRH39_001045"
     CDS             complement(10303..12198)
                     /locus_tag="KRH39_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBG8510965.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKIDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTS
                     TVEVKRAKDSTNVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYTLTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            complement(12823..12966)
                     /gene="scfA"
                     /locus_tag="KRH39_001046"
     CDS             complement(12823..12966)
                     /gene="scfA"
                     /locus_tag="KRH39_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBG8510966.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(13032..13394)
                     /locus_tag="KRH39_001047"
     CDS             complement(13032..13394)
                     /locus_tag="KRH39_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBG8510967.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(13452..13745)
                     /gene="yajC"
                     /locus_tag="KRH39_001048"
     CDS             complement(13452..13745)
                     /gene="yajC"
                     /locus_tag="KRH39_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBG8510968.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(13879..15000)
                     /gene="tgt"
                     /locus_tag="KRH39_001049"
     CDS             complement(13879..15000)
                     /gene="tgt"
                     /locus_tag="KRH39_001049"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBG8510969.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITSIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(15079..15564)
                     /locus_tag="KRH39_001050"
     CDS             complement(15079..15564)
                     /locus_tag="KRH39_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510970.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(15576..16601)
                     /gene="queA"
                     /locus_tag="KRH39_001051"
     CDS             complement(15576..16601)
                     /gene="queA"
                     /locus_tag="KRH39_001051"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBG8510971.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGAKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKANGNNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(16919..17938)
                     /gene="ruvB"
                     /locus_tag="KRH39_001052"
     CDS             complement(16919..17938)
                     /gene="ruvB"
                     /locus_tag="KRH39_001052"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBG8510972.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(18045..18647)
                     /gene="ruvA"
                     /locus_tag="KRH39_001053"
     CDS             complement(18045..18647)
                     /gene="ruvA"
                     /locus_tag="KRH39_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBG8510973.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEST
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(18640..19149)
                     /gene="ruvC"
                     /locus_tag="KRH39_001054"
     CDS             complement(18640..19149)
                     /gene="ruvC"
                     /locus_tag="KRH39_001054"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBG8510974.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(19462..20091)
                     /locus_tag="KRH39_001055"
     CDS             complement(19462..20091)
                     /locus_tag="KRH39_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510975.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKDEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            20294..20617
                     /locus_tag="KRH39_001056"
     CDS             20294..20617
                     /locus_tag="KRH39_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG8510976.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            20885..21739
                     /locus_tag="KRH39_001057"
     CDS             20885..21739
                     /locus_tag="KRH39_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510977.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     VTVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(21925..23361)
                     /locus_tag="KRH39_001058"
     CDS             complement(21925..23361)
                     /locus_tag="KRH39_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBG8510978.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNELLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSGKLFNGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTKGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNVTDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKILGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(23433..24815)
                     /locus_tag="KRH39_001059"
     CDS             complement(23433..24815)
                     /locus_tag="KRH39_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG8510979.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(24951..25856)
                     /gene="garR"
                     /locus_tag="KRH39_001060"
     CDS             complement(24951..25856)
                     /gene="garR"
                     /locus_tag="KRH39_001060"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBG8510980.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTVLPNGTIVQDILFGMDGLTKTLKEGSIVVDMSSVTP
                     TESIFCANKLKNMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFYIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            26252..26917
                     /locus_tag="KRH39_001061"
     CDS             26252..26917
                     /locus_tag="KRH39_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8510981.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYKTTLIHMETPKYINLEDFC
                     NNK"
     gene            27025..27573
                     /locus_tag="KRH39_001062"
     CDS             27025..27573
                     /locus_tag="KRH39_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510982.1"
                     /translation="MIFLNKDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFDENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDSKVSEGKYTYGNKNCK"
     gene            27835..28143
                     /locus_tag="KRH39_001063"
     CDS             27835..28143
                     /locus_tag="KRH39_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510983.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(28305..30095)
                     /locus_tag="KRH39_001064"
     CDS             complement(28305..30095)
                     /locus_tag="KRH39_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510984.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(30095..31822)
                     /locus_tag="KRH39_001065"
     CDS             complement(30095..31822)
                     /locus_tag="KRH39_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510985.1"
                     /translation="MKLILSYLKNYKLLIVLNVLAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(32067..32630)
                     /locus_tag="KRH39_001066"
     CDS             complement(32067..32630)
                     /locus_tag="KRH39_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBG8510986.1"
                     /translation="MLEVVNMRKVVLYIATSLDGYIAEENGSVSFLDEISSEFKNEQE
                     DDYNTFYDTIDTIIMGNSTYVQIANELSPNEWYYKGKECYVYSKTINGATENVKYTNL
                     EPKKLIEQIGKEKEKKDIWIVGGGDIVKLFMKDNLIDDYYIYILPIILGRGISLFKSG
                     IDKINLNFKKASNIGELVKLEYSKKQK"
     gene            32928..34040
                     /locus_tag="KRH39_001067"
     CDS             32928..34040
                     /locus_tag="KRH39_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG8510987.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(34405..34914)
                     /locus_tag="KRH39_001068"
     CDS             complement(34405..34914)
                     /locus_tag="KRH39_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG8510988.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(34916..35563)
                     /locus_tag="KRH39_001069"
     CDS             complement(34916..35563)
                     /locus_tag="KRH39_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510989.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(35577..36749)
                     /locus_tag="KRH39_001070"
     CDS             complement(35577..36749)
                     /locus_tag="KRH39_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG8510990.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKEGLCAFNMETGLVDNSLCIGRKGRIGCNIAVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            37017..38393
                     /locus_tag="KRH39_001071"
     CDS             37017..38393
                     /locus_tag="KRH39_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8510991.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
BASE COUNT        12426 a         6813 c         4517 g        15300 t
ORIGIN      
        1 tttacttaat atttattttt caattaaact cttaatctta tctattacag atgaggctat
       61 tcctccacct acttcaaaca actttgatga atttttacct tttaatgacg attcttgtga
      121 tttatctaac gtatttgtag caagtataac tggtgaattg tttttagctg ctaatggtcc
      181 aactgctaaa gcatctataa gtttgtcttg atttgcaact acaactccat ctagtttaga
      241 ttgagagtag aatttatcta ttactttggc attcgtctct tgtctagttt gacctgccac
      301 cctatttttt gatatatctc tgtttgctat ttttccaact tgttcaatag ccttgttaga
      361 tattttttct attccaccaa taaagtatat atttgacacc ttatttgatt taataaattg
      421 ttcagtttca ctatctacac catctttttg tgtaagtact attggagttt tttctttacc
      481 agccagtgat gctattgata atgaatcagc ttctccattt cctgctccta tatacaactt
      541 gcttacatta ttcgtcttat ctatttcttt tgctatatta atagaggttt gatatcttgt
      601 atctccacct attctttgta ctgatatttt tgaatttaca gctttgatag cttcaacaac
      661 tgaatttggc attgagttgt ttccacctat cacaattact tttgatggat ttagcctttt
      721 caattcttct acagtttttt gaggaagctt ttcatttgat gataaaagaa tcggtgagtt
      781 ttttacacta gcaagtggtg tagctgtaag cccatccacc atacttttat cttctccatt
      841 tactattaca actgtatctg attttgtcca accactttga gatattttga tagctgtttc
      901 aaatctatca gcacctttta attttttgac tgtgacatta gatgtagaat cacttgaatt
      961 tcctcctcca gagcttccac cactagacga accgcctgaa ttacttcctc caccattatt
     1021 tccaccattg ccattatctg gtggtaattc gtccggtttt gataattcat tttcaactat
     1081 gtattctaaa ctttttggaa gtgtaacttc aacatatttg tttattgtag cttttcttcc
     1141 cttagttgtt ttttgtgcta atccaagttt taatattaca tttaaaccac tttcttgtgt
     1201 ttctttacta acaatagaat agtttatcct aatattgttt aataaatatt caagtgcttc
     1261 acttttctct accttatact tttcagggtc atttttacta gaaccagttc caccaattgg
     1321 ttttacttca ctctcattaa taagggtaaa tattaatcct tcatcagagt ttatattctc
     1381 ataatccact gttttattgt tatatgttac aatttttaag gctggtttat cgcttgttcc
     1441 ttttccaaaa actttatagt cggcatttaa gttactgcca gattttttta tagatattct
     1501 tcctatattc aatctatttc cgtctagtaa atcttcattt gatgtaacat atatatctaa
     1561 tgtagttgta ttacttgatt tgttgtattc tgctctacaa gtagcatttg catttccact
     1621 ttttatctga gattttatat tatcagacca agtaatcttt tctttctgct cattatctac
     1681 tacttcaacc tcaccttgta cttttatact ggcttgaaat gaatatacta tattttctac
     1741 tgagtccaca gatatatcta tagtatttac tttctcatac gtttttgttc cttgtaaaac
     1801 tggagctgca tatgcacatg tagcactacc agttacagct actgccatag ttgatactgc
     1861 tatagttctt ataaaagatt ttcttttact cattaacacc cgtccttatc tgtcattaac
     1921 taagtggata aatttactta tccacctagc ttttatattt aatttataaa ttaccctaaa
     1981 cttatagtta catttatata ctttactttt aatatctact attttaaaac tattctagta
     2041 cctaatcata ttagatatac catgttagta ttagtatttt tacgttttta gagttccacc
     2101 tactatttat ctctaacaat attacccttt actttattgc ttacagttac aaccttctta
     2161 ttgctgacat caatagcatt tcttgtagct tttctagctt tactattact atcagtaaga
     2221 cttattgtct ttaactcatc ataattctta gctcctacag gtacatacat tgtatcactt
     2281 acaaatcttt gacctactgg agtatctcct tgaaggtcat tgtatctata tagttcagcc
     2341 ttaaccttat cacttaaaga atcaaagttt tcttttggca tatagtaaac ccatgtacct
     2401 ttactgattt cacctaattc acctttttct ggaacttctg caaataaaag tgcttctcca
     2461 gatatagtct tattgttttt atctataagt agtggtaaat taaatgctgg atttccactg
     2521 tattcacctg taataactat tgaacctgct ggaatcttac ctgtttcatc ttcacttgtg
     2581 ttagagtcat cactttgata tacataatct tctgctaact ttccaattcc attagtacga
     2641 gtttggtctg cattattcat tgtgccaatt tctatattat cacctggagg tcctattata
     2701 tctaattctg caacttctat attagactta ttatttgaga ttagttttac atatctagct
     2761 tggtatgtcc aaagtttacc atctttatta aatgtaactc tagcttcatt atcttggtct
     2821 tttcctccac caactaaatc ttgtccatat tcaaaagttc cttgacttac atcttcccag
     2881 ttctttttat cattacttac ttgtatcttg taattagtta aaggattgta tgttgtgcta
     2941 gtattctttc taaagttaaa taatcctttt aatgagaact tcctagcttc tgtctctggt
     3001 tttgtatatt ttagtcctac tataggtaat gtttctttca tgtctactat tatatatgga
     3061 ttttcttcta catttatatc agggtctttt tgagggtctt tgttccactc tgctttactt
     3121 attatctttc ctttatatgt agtacttgca tcattatctt ttatattatc aatttcagga
     3181 ttttgtaatg gtccatgtga gtcattttca gttctaacat caccgtcaga aacagtattt
     3241 gttatgaatg accatgctga tttatttatt ttcccttcat ctgataattt tacagtacca
     3301 acttcaaatt cttctgttgg atttaggtga taatcatatg caactacctt atatgtaaat
     3361 gctctgttat taagaggaga tatattatca gtaaatttag ctttactatt tccttttgca
     3421 tccacaaatc caacagttct gtattttacc tgtgttccat tttcaccatc taaataattt
     3481 ccatcacttc ttattatttc gtatcctagt atcttatctt tatccttatc tacatctagg
     3541 tctatagata ttttattaga tttaactaga gaaccactct gtattccatc agaaaaacta
     3601 gaacttactt tagtatcttt tgccatacta gctatatttt catttaaaat ctttcttcta
     3661 gcttcgtcat ttacatattg tatctttctt gattcttttt cgtagttctt actagccatt
     3721 atactgttta attctggtgt aatttctact ccccatgcct taaagtaatc tcttaaatct
     3781 ttttcaacaa cacttgatgc cattgctact agcatttggt cttttggtaa actattaatt
     3841 gggtcactac ttgaaagtgc tctgtatttt cttgaaatct ttgcatagaa tgtgtcatta
     3901 cttaaatcag catctgagtt attctttaac attgaacccg taaaatcatc atcataagct
     3961 aaatgtaatt gccataacat tccaagcctt tcaaatacat tgcttggtat tcctaagcta
     4021 tttgaagtta catgctcata tatcttatcc attgttgaac cttcaagtct tgaatgtgtc
     4081 ttatcatcaa aggtctgagc aagtaaagca agtacattgt tactagtttc gctatatgtc
     4141 atctttccaa tatctgtaac atgtcctatc tcatgaccta ttccccatcc aaataagttt
     4201 cctcctgtag cttttccact atcatctatt tggaaaggta ctccattcat aagccctgga
     4261 actgaaccat attctactcc tacatgtaat ccagaagcat acataaatgc tccaataaac
     4321 attctttggt attttatgtt catacgagat tttggagcca tatcattttt agttaagtta
     4381 aattcattat catctacagt tccattacca tctaaatctt gtgttttaaa tactcctctt
     4441 tctgagtaaa ctaaatccat tatttgttcc catgcaagca tactattata taatctatct
     4501 acttgtgtat ttaaatcagc atttcctttt gatatccctt gatatacagc tgttgcagga
     4561 acatttaatg ttactttatc actttcaata tctgtagtgt ttaacacacc agttttttct
     4621 gaatatccat atgaattacc aacatttgtt cctggataat atggttctcc ttttacaaca
     4681 tctttatatt tagctaattc ttctatatat gcttttatct ttttcttaga ttcagaaact
     4741 cccgcaggac tcttatcatt tattgttcca tttagattaa gatgtggtat cttagtagct
     4801 cctgctacac gtacttttat atctcctcca cttggagtat cttcagcgta tcttatgtat
     4861 aaacttcctc cacattctat gtctaaacta tgaagtttgc ttaattgaat tacatttttt
     4921 ccagttttaa gatttatttc tccactctta tatgcaccag attctccata gtactgtgta
     4981 tatatcaact taggagtttt tccactttta cttccaacat atactgtgat ttcatcccct
     5041 gctctagcag ctactcctaa tgcctgccag tcattttgca tacctaaatt attggaattt
     5101 acagtatact gtccgttttt atctttatat tttcctgatt tatttatttc ttgatttaca
     5161 gtagttacat cagtaagtgt ggctctatca ttatataagt cttgagctaa ttttaattct
     5221 ctatcaataa ctgtcttata tggatgatat tctccactag ctttatctgg agtatttaat
     5281 ctatctttta attcagttat tttttcttga gttacatctt cttttaattc aacttgtagg
     5341 tcatccataa atagatttct tacatcatct tctatactgt catactcata aaacttaagt
     5401 tctgatatat tagacttata tgaataagat gaacaaacac ttacttgtat tttagtagca
     5461 gttattggtt cttttaatct aaccattaca tacttattat tatttacagg gtctgtcttt
     5521 gtttcaaaag gaacatcccc cagattgtgt aattgaccat tttcatccca atatcgtacc
     5581 tcaaagtcat ttattgtctt gtcagaaaca tcaaaacgag gtgtagccat aaaagtatcc
     5641 atagtatagg atttatcaaa agttattatt ggtcctctat tcgttccctc agctgcatta
     5701 tacgttggga atgtccaatg tgttgtaaag tccccatcta ctacattgaa tttatcaaat
     5761 ggatttttat cactatattc acttttgtca tatccatctg gataaacaac atctacaatg
     5821 tgatttgttg gtattccaaa tccaccattc tctttaggag tattaattaa tctatagtta
     5881 ggtgttgttg gtgcatccat gctttcagtt gtacctatat atgttttgga cattccacta
     5941 gttcccaagt ggttagtagc tgtcattcta acttcatatg ttgtattatc cttaagacct
     6001 tctatctttg aacttgtccc acgaataagc ttatcattgt attttatcca agacccatta
     6061 gagccttttt ctttatagta caagtcaaaa tctttagctt gtttatgatt tttccagctt
     6121 acatcaagag atttaaattt acctttaaca tttatacctt ctggtggttc tggttttgtg
     6181 tctggaacaa cttgaaattc taatatacta tctgggctaa attgtatagg tttatagttt
     6241 gaatctacat tttctggtac tccatctaag ttttctggtg ttgcatcttt ataaccacta
     6301 gaccaatctc catttaaaga ctgtatgcta actctatata gtccatatgc ttctatatct
     6361 ctaacttcta ttgtattttt actagtttgt aattttttag tagataatac ttttccctta
     6421 ctatcaattt tatctagtat aagttcgtat gctgatacat ttacttcatt attccaataa
     6481 aaagatactt tttcatttcc aacagcagta ctagttgata ttttatttat tttaggtatg
     6541 ttcatctctg gctttggtat ttctttatat acattgttta aaaattcaac ttttgatatt
     6601 tctgcaagat ttctatcgtt tgctgttgta gctgttattt ttatagttat cttttttact
     6661 gctacttgtt taccaagatt tattactata tcgctatttt gaacatctgc atatgtttct
     6721 aatgctgacc tactatatgt agatgttgta gaattatcat ctttactgat attaccctct
     6781 tcttttggag tatcttcatc tgaattaggt ttatccattg atgaattttc tttatcatta
     6841 tttttatctt gattggtatt agagtttgct tcttgtttat cgctattttc ctgttgttta
     6901 tcattatttt catctttatt atttgagtta ttatcagtct ggtttatatt ttcatcttta
     6961 ttatttactt cttcttgatt tacttcttct tgttcgttat ttacttcctc ttgttgctct
     7021 tcatctttta agtttttcat atattctttg ttttcataag gaatctgttg aacattacca
     7081 tctttgtcag taacctcaac tattcctttt gctggagcag taccttctgg agcttttata
     7141 ttgatgactt ccatattaag tgcttctgat aaagctatat ccattactgc tggtttttcc
     7201 tcagatattt tatcattttt tgaagatatt gtaactccat tattttctcc actaaataag
     7261 ttttctacat taccattaat atctacatca ctatttcctt ttaattcaac cttacttgta
     7321 tctactatag gattggtaga tactatactt tctaggcttt ttgcatcttc actttgtcct
     7381 aaatttttct gtacataatc taagtcaaca atatctacct ttccatctct atttaaatca
     7441 aattctcttt tatttgaatc tatattctct aaaaccttat tataatcact cttatctact
     7501 ttaccgtctc catttacatc acccattaag aataattcat aatttaaagg tttattttta
     7561 ttatctacac ttgctctatt gcttaataag actctttgtg aataacttga tatatcaatg
     7621 tctttaattt ctttgttctt aaatccagct ccagagactt ctacattgta ttttctaaca
     7681 ggtaaattat taaatacaat attataatat gatatattct catctttatt ttttatttca
     7741 cttctacttg cagataattt ttttatagta tattctatat tatctgtccc aacatttact
     7801 gttcctgaat ctttttctcc aagctttatt attccactat tgttactatc atcttttaat
     7861 cgtaactgca tattggttat actttctgac tcgctatttt ttattggcag tgaaaaattt
     7921 aaatctactt caagattccc ccttaacttt tctgtaactg atttttcaac tgcccttagc
     7981 ttcccctcta atataggttc tgcaaaaacg tttagtggtg cactacacat agatactgct
     8041 agtacacaag atattgcctt tttcatgaaa caacaatcct cccttaataa atatctaatt
     8101 tttcatgaat ttaaatacta taagtttcag acaataaaaa aagacactca aagagaatct
     8161 taattataaa ataaaaagac tttactcttt ttagcaactg gctgatatgg ctttaaaagc
     8221 tttaatccct atagttttgc gtcattagat ttccctaatt ttgccattta attttcacta
     8281 ctttcttacc cattttatac tatatttaaa ctttttaata caattttttt aaattaaatt
     8341 ataaaaaaat aacatttata ttaatttatt aatataaatg ttattttcca ttataattta
     8401 attatttata tagcttcctt acaagcataa ttttgtgtat attttcttat tctttatatc
     8461 ttccataaaa ttcatgtgta gcatatagat tcttatcccc tgttatagct ataccaaatc
     8521 ccattatttc atacccttta tgcaacatat tggtaccttc ttctggattt gtcttccact
     8581 cattaaaaat aaagtttcct atatcatctg catctctatt tgtatataca ggtttatctg
     8641 gaattttata tttaattata ttttcacctt gaactgctat atatccaggt ttaatctcac
     8701 tccaatctaa gtatttcaaa aaagtagaat atgaattttt tccatttatc gtatgagata
     8761 aagtcttctt attttccatt aaattagacc aatcatttgc tagaccctct aatctacttg
     8821 ctacagataa cgctcttact ccattctctt ttctatactg ctttgtaaga gcatatactt
     8881 taccctttat tctctcttta aatgctctat catttggatt gtaatataaa ccttgttgat
     8941 ttggtgaagc ttgatttata cattgcttga taacattctg atttatgcct cctagtacag
     9001 tcaatttgtc tgcaccactt aaaatactct tgttactgcc attttcaact aaaactacag
     9061 gagcattttt tgctattgta gaggcagcta aagcgtctgt taattgtaag cctttactta
     9121 gataaaattc tttactattt ttataaaact cttgtataac tatcttatta gtttcaaatc
     9181 tatctgtgcc acccaatcta atagaatttg tgctcttaac taattcgtcg ctgattattt
     9241 cactagaacc aagcgcataa ctctttaagc ctgtcgtgtc aaatcccaca ctatttccat
     9301 ctgtaagtat tataggtact ccatctctag cagctactgg tgatgcactt atcgcatctg
     9361 cttctcctgt aaatccattg gctataatta ctttatctat tggcttaatt tctttgattt
     9421 tttcagctat ttttatgctt gtatcaaatc tattttcacc aaatattctt tgtacaacct
     9481 tctttttgga tagtaactga ttctcaacat ttttacttat tgttgattct gaacctatta
     9541 tatatacagt ctttgctttg tttaatcttt ccatagttgt gtttggaatc tggttttgct
     9601 ttgtcatcaa aataggagca tttaaggctc cagctaaacc acttgcgctt agaccgtctg
     9661 ctaagctatt atctgaattg attagtattg ctgtattgta gttttgctta tctgctattt
     9721 ttgctgctgt ctcatatctg tcattccctt ttatttgctc aactgatgtc aacgcattta
     9781 ccataggagt atttaccatt actaaaaata gtgtaagtcc tatagctaat atctttttac
     9841 tattcttcat attattttct acccccaatg tacttttcac attttttctt agtactcata
     9901 cttattttta atcaaaagtt acagttttgt taccatattt tatatatgat accatagtta
     9961 tccatacaac tcaaacaata tacctaaagt ttttattctc tataaattca aaacattata
    10021 atttatttta tttaattaag ttataagttc aaaataattt atgttaataa gatgtttaat
    10081 cattataaga agtatctttt tataataatt tttgcaatat gagtttctca acattataat
    10141 caaaaaataa aataaggtat ctcaatctaa ttctaaatga gacaccttat ttttattttg
    10201 ttatttatat attttataat atctacacta tgtcttacat attaacacat tgtataatct
    10261 atattatcta aataaacttt taatattaat tcctatatct tattagttct tagtaaataa
    10321 gtttattttt tctgctattt tttcaatagc gtttttagct attccttcac ctatttgtac
    10381 taacttagta gcattcttaa gtcttaactc aactacttct tcttgtgatt tagaaagttc
    10441 ttctgtagtt aatactattg gagcttttga actagctgca agtggtgctg ctgtaagtgc
    10501 atcaactaat tgtccatttc cacctacata accatctttt gctacatatg ctttagttgc
    10561 acctgcatag tacttattga ttactttagc attagtatct tttctatttt ctccagctaa
    10621 tctttcaact ttattattta aatctatatc aactaatttg tcttcaactt cttttgatac
    10681 agaattttct ccacctacta tttctaattc ttttcctttt aataactttt cagcatttgc
    10741 atctaattcg ttacctgtaa ctattattgg agcaccaaat tgagcagctc tagctgatat
    10801 actcatagca tcagcttctc cgtttccacc tactacaaaa gcagtcttaa atgtatcttt
    10861 taattgtttt gctatttcta aagaagtttc aaatctatct tctccagaaa ctctctcaac
    10921 tttacttaca cctaatttag aaagtttttc ttcagtttct tttgatactt tagattctcc
    10981 acctactata tatattttct tagaagatag attacttcca agtactctta atatttcagc
    11041 ttctatttct gatggtagtg aatctttttt agataataat attggtgcat ttttttgaga
    11101 ggcaagtggt gctgatgcta atccatcaac tatagcatct tctccaacta aaattatagc
    11161 ttctgcttct ccatctttga atctctcttt actaacttct actgctgttg taaatcttga
    11221 atcaccagat aaaacttcta cttttaattc ttttccatct tttacatcag ttaatacttt
    11281 gtgtaagtct ctaactaact tatggttttt acttgaaaca cttattgttt ctggcttttc
    11341 atttgcctta cttaaaacta tatcaaaact tgatttttct ggcttatctt tatcatcact
    11401 atcttcttgt tcttgagtaa ggattgcttc attttcaaat gttaaagtat atccattttt
    11461 aactttatta actatttctc ttcctaatgt agttaatcta attccatcaa ataattcttc
    11521 tgctgatatg tcttgtaaat ctgcgttttt aactgttatc gtatgtaatt cttttgaatc
    11581 tatgtcacta taactctttt cataaccttc aaaagtacct tcttctgatg ttatcacttt
    11641 tgagaaatct aaatgttggt ctcctacatt taatgtaata acatttgttg aatcttttgc
    11701 tctttttact tcaactgtac ttgtagattt tatagttgct gttaatttgt tatcagaatc
    11761 ttctgctaaa gttgcattgt aagcattaac tgcatctact atttcttgag cagttttgta
    11821 gttttcaatt ttatagtcaa ctacttttcc acctaacact tgatgccctt tatcttgaat
    11881 agtaactttt aaactttgcc cttctgttaa agagtttatt ttattttgta attgagttgc
    11941 attagttaag tttacattgt ctatttttat atcgtaaact ctatcattta catttgctcc
    12001 tgcttttgta tcttcaaact taacttctaa tgcttttcta acctcttcga ttagtttagc
    12061 agaatctttc gcacttactg tgtaattttt atcaacattt tctgtcacag tattatctgc
    12121 gaatattggt gctgctgcac tcataactgt agcacctgcc attactattg ccatacttct
    12181 ctttttgttg cttaacatat ttatgtcctc cgaatattat tttttatttt ttatattcgt
    12241 atttcccaca cataaataat attatatata atattatttt tcaatatatt ttatgtgttt
    12301 acataattta ctaacacaaa cactaataat atcttcttga accccatacc cacttagttg
    12361 tctataatca tgctcaaaga tagaaatccc tattttatag tgattttcta ataaacaatc
    12421 atatactaac aaaagtattt aaactccatt tttatatact actatgcact aatttttgct
    12481 ttactcttat tacttaaata tatatcacta aattaatatc tatctccata tattaaaata
    12541 tataacatat tataaaaatc atcataaatt agagtttaaa attaaaaatt attctaatac
    12601 atgtaattga taaaaaataa aatataagag cagatacttt aaaataaatg ctcttatatg
    12661 attagatatt cttgcataat taaacgataa gaaattaaat ttgattttac ataaatagaa
    12721 tgcatatttt tatataataa ttgaaaataa aagaggatac ctcataatga taaaagaatc
    12781 actttgagat accctcttac aaacaactaa atcaataatt ttctatcttt cacactcttg
    12841 atttgcaact gtacaagaag ttttacaagc tgattgacaa gaagtttgac actctccaca
    12901 tccacctttt gcagcacttt gctttaaagt agcttgagat aaagttttta catgtttatt
    12961 ttccattttc aacgcctcca tttctaattg tttcctattg attatatcat aaaactactt
    13021 taatataaac tctatttaaa gttgacaccc aatacgcctc caatgcctcc tgctgctgat
    13081 attagcaata acttataaat catccctact tcaaatacaa acttttcttg tgctaaaaac
    13141 acagtcaaaa atatacatac tatgtacaaa agtcctacta aaagcccata cataagtcct
    13201 ttttctttca tgtgttttga agtgtaaaac cctcctatag ctgcagaaat agtagttata
    13261 aatgatgaga ccatagttat attatctcca ccaatatctg taaatgtcaa aaataagtta
    13321 tacactaata aaacagccaa agttattata tatgcatatc ctagaccttt aaaaatataa
    13381 ttagattttc ccataaaaaa acctcctctc aaattaatat atattcgaga aaaggctttt
    13441 aaattactaa tttagtcttc ttctatttct tcttttttag atttaacagt tcctatagcc
    13501 catttttcaa atggaacttt agttctattt ggtcctaatt ctaatattac aacatcatct
    13561 tcaactttag ctacattagc tactattcca cctattgtaa tgaccttgtc tcctacactt
    13621 aggctacttc tcatttcttt taattgtttg tcttttttct tttgaggtct aatcattagg
    13681 aaataaaaga ttgcgattac tactacccat agacctatac tcataattat ttgttgctgt
    13741 ggcattaatt tttcactcct ttgtaaatta tactcttttg taaatcataa taaatgttta
    13801 tataacaatt tactttctct atctttgagt actattgaat attatatatt aaaatgatta
    13861 taattaatag tgcaaaacct atatttcata tccatatttt gcaaaaaatt catttctaaa
    13921 atcaagtaat ctatcttcca ttatcgcttg tcttatttgt ttcattaaat ttaataaaaa
    13981 gtgaagatta tgagtagtta ttaatcttgc acccagtatt tcatttgctt ttataaggtg
    14041 tcttatataa gctcttgaat agtttttaca agcataacaa tcacattcag ggtcaagtgg
    14101 agtaaagtct tcagcataag ttgcatttct aactactact ttaccttggc tagtcattgc
    14161 agttccattt ctagcaatac gagtaggtag gacacaatca aacatgtcta ctcctcttat
    14221 aacaccttcc actaagtcat ctggactacc aactcccatt aggtatcttg gtttatcctt
    14281 tggcataagt ggagttgtat gctctagcac gtcatacata agtggttttg gttctccaac
    14341 actaagccca ccaatagcat atcctggtaa atcaatacta gttatttctt ttgctgattg
    14401 ttctcttaag tctttataca tcccaccttg tattatacca aataaagctt gtttgtcggt
    14461 attattatgg gcatctttac atctttttaa ccatcttgtt gttctttcta aagaattttt
    14521 tacatattct ctatctgatg ggtatggtgc acactcatca aacgccatca ttatgtctga
    14581 acccaatgca ttttgtattt ccatagcttt ttctggagtt agaaaatgtt ttgaaccatc
    14641 taagtgtgac ctaaattcta caccttcttc ttttatcttt cttaaaggcc ctaagctaaa
    14701 tacttggaac cctccactat ctgtaagtat tggtttatcc caattcataa acttatgaag
    14761 accaccagct ctttttataa gttcatgacc tggtctcata tataaatggt atgtgttact
    14821 aagtattatt tgtgaaccaa tctcttttaa ttcttctggt gtcatagatt ttacagttgc
    14881 ttgagttcca actggcatga atattggtgt ttcaataatc ccatgtggag tgtgtaatct
    14941 tcctaacctt gcaccactct gcttacaagt ctttatcaat tcgtatctta ctgcgtacat
    15001 aaagttctcc ttaatcatat ttatttctca ttatttctta agatatattt gtaataagct
    15061 attcatataa tttattgtct aaatagattt atcttcgtct tctttgttct gttgttcctt
    15121 aattgcttct tcatcatgaa tactcattat attaagagca ctcttgtctt ttgttactaa
    15181 aaactttttt atagcaaata gtcctgctat agctataact cctattaaaa catctatcat
    15241 accttcattt ttaggcagta gcaacatttt tctagctatt gcgtaaagta atgcctcaat
    15301 aaatgctcct ggtatatgaa gagatagcat tactacaagt tcaacaccta ttactaaaag
    15361 taatatttgt gctaaaaaat cattaagctg actgtattgt actggatttt gaaaatcaac
    15421 tatatatgct ccccacataa gtcttagaac atctatagtt cctaaaaata ctgctattag
    15481 aactactact gctaaaacac gttcaaatat aaaagcaatc tttaatatag ggttattctt
    15541 tttttgtttt tgtaaactat gcattcatta cctccctact ttataatcat agcatctcca
    15601 aaactgaaga atctatatct ttcttttact gcctcattat atgcatttaa aacattatcc
    15661 tttccacaaa tagcacttac taacattatt agtgtagatt ctggtaaatg aaaattagta
    15721 ataagtttat ctaagacttt aaatttataa cctggatata taaatatttc tgtccatcca
    15781 ctagtttctt tcatctttcc atcctcatta caagctgatt ctatagttct acaactagta
    15841 gtaccaacac atattacatt gtttccattt gccttcgctc tatttatttt gtcagctgct
    15901 tcttggctaa ccatataata ttctgaatgc atcttgtgat ttaaaacatc ttctactttt
    15961 actggtctaa aagtgcctaa accaacatgt agagttacaa aagctatatc tacacctttt
    16021 tcttttattt tatttaacaa ttcttctgta aaatgtaatc ctgctgtagg tgctgctgct
    16081 gaaccattat gctttgaata aacagtctgg tatctttctt tttcatctaa tctagcagtg
    16141 atatatggag gtagtggcat atttccaagt tcatccaaga tttcttcaaa aattccatca
    16201 taatggaatt ttataattct tgaaccttca tctgataaat ctacaacttc tccaattagc
    16261 tttccatttc caaaagaaaa tttagcccct acttttgctc tttttcctgg ttttactaga
    16321 gcctgccaag tatcttcctc tgttcttttt aataataaaa actcaatttt acctccagtt
    16381 tcaagcttct ctcctattaa tctagctggt ataactctag tattatttaa tactaagcaa
    16441 tctcctggat ttaaatattc tattatatct ctaaatactt tatgttcaat attacctgtc
    16501 tctttatcta ataccatcaa tcttgagcta gctctatctt ctataggaac ctgagctata
    16561 agttcctgtg gtaagtcaaa tttaaaatca cttgttttca atgttaaact tcctctctta
    16621 aaatttctcc agctacaata atatcaaaaa aacaacttat tttccacaat attaccataa
    16681 tctatcaaga aattgctatt tttaacagac accatatcca caatatatag atatttttcc
    16741 acgatataca cgtcttatcc acaatatata gatacaatac acacaatctt tatcgtgttt
    16801 atccacagta tctacagata taattaccat tttgtggata tgtttgaatt tttagccatt
    16861 ttagaaatat tttaatttaa tgactatttt attttataaa tactcgtata atctattttt
    16921 atttttcatt aggatatgga atttttaagt gttcatatgc taatggcatg gctactcttc
    16981 ctctaggccc tctatttata aatcctaatt gaagtaaata tggctcatat acatcttcta
    17041 ttgtatttct atcttctccg atagaagctg ccaatgtatc taaacccact gggcctcctc
    17101 taaatttttc tattatagtc ataagaagct tctcatctac aaaatcgaga cccaaactat
    17161 cgacacctaa aagttccaga gcatcttttg ccaccttatc agttattttt ccatcagctc
    17221 taacttgtgc aaaatctcta actcttttta gtaatctatt tgctatcctt ggagtccctc
    17281 ttgaacgttt tgcaagttct aatgctccat tagactgtat ctctgcatct aagatgcttg
    17341 aagacctaag tactattttt gaaagttcat caactgtata atagtccaat ttacaaataa
    17401 caccaaatct atctctaagt ggatttgtaa gcataccagc tcttgtagta gctccaataa
    17461 gtgtaaattt aggaaggtca agtcttatgc ttcttgcaga tggccctttt cctattatta
    17521 tatctaggca aaaatcctcc attgctggat ataaaacttc ttcaacactt ctattgattc
    17581 tatgtatctc atctataaat aaaacatcat tttcatttaa attcgtaagt atggctgcca
    17641 aatctccagc tctttctatt gcgggtcctg acgtaattct taaatttacc cccatctcat
    17701 tggctattat acttgctagt gtagtttttc caagacctgg tggtccatat aaaagcacat
    17761 ggtctaactg ctcatttctc gatttagctg cttctataaa tatactaagt tgttctttgg
    17821 atttttcttg acccaaataa tcttctaaag tttttggtct taaactgttt tctatatcta
    17881 tatcttccat cttcattgta gaagttatta tcctattttc atcttcaaaa ccttgcattt
    17941 atacccctcc tctcgtactt ttgaaaatct actttatata ttttcaaatt tacataaact
    18001 ccttaaatat tgttttaaaa tccaaatatt ataaaatcca aatattattt caaggattta
    18061 ctcattatat atgtaagtgc tttctttata atagcttctg tattcatacc atctttttta
    18121 catttttgaa ctgcctcttt tgattccgac aaagtatagc ctaaagccac aagtgcatct
    18181 acagactcat cttgtgatat caaggtaggt ttttgtgata ataacgttgg ttcaaattct
    18241 atgttatttt tgtctacctt atccttaagc tctaagacga ttctttctgc tgtcttttta
    18301 ccaacaccag gagctttgga taattttcca atgtcttcac ttaaaatata tgctcctaac
    18361 tgtgctggtg atgcaaatga tagtattgaa agcgccacct taggtcctat tttagatacg
    18421 gatgttagta gttcaaacat cttaagttct tccctactta caaaaccaca aagactcata
    18481 tcatcttctc ttacaattaa tttagtatat atttttgtgg attctcccac ttgtaaattc
    18541 attattgtat tggatgatac atttatttta tatcctatcc cattattttc aacaactata
    18601 ctgtctatat aaatttcttc tactgtacct tttatataac tatacatact taccacctat
    18661 attctttaga gttttttcaa gtttatttga atgagcatgg caaatagcca cagctaaagc
    18721 atctgcaaca tcatctggtt ttggaacttt ttttaaactt aaaaatgaag ttaccatctg
    18781 ttgaacttgt gccttatctg ctctcccata tccaactaca ccttgtttta cttgaagtgg
    18841 agtgtattca tatacaggct tcccattatg agcacatgcg agcatagtaa cgcctctagc
    18901 ttgtgcaact gttatagctg tttttacatt cttgttaaag aataattctt ccattccaac
    18961 ttcatctata ttgtaattct ttactattgt atcaattcct ttatatacaa gttccaatct
    19021 tctcgatata ttcatatggg caggtgttgt aactgctcca taatcgatta ctttaaactt
    19081 gctatttttg tattcaatta taccatatcc aactatagct atacctgggt caatccccaa
    19141 tattatcatt ttttaaaatt ccccctaaaa ataaaagaaa taccaaaatg tatttctcat
    19201 aaaaacttat gttctaaatc attatatcat aaataatatt aacaaatcat aaaatgctct
    19261 atgtaaaata taaaatcaaa taaaaaagtt atatcaaagc aagattttta cctcaatata
    19321 acttttatat ttagtacaaa taaattattg catttttata ggaattaaat gtgactacaa
    19381 ttttaatact aatctattta atattaaatt atttattatt aacttactta gtatttaact
    19441 tgaaatagta ttaatttact attaacttgc aaagtcttct aatcttgaat aggcctcttc
    19501 ttcagtatcc ataactacac cttttttgat ttcatcttga actgttttag gaagtgaatc
    19561 tactcttatt tttgtttttt ctactaatgt tttacttcca ctatcatcat atttatatag
    19621 tgctatattt ccattatcat tttcaatagt atatttacct tttatagtaa catccattgc
    19681 ttgtacatct ttattatcac tatatttaga ttctaccaat atgatttcat ctttatccat
    19741 actctttatt tttttagttg gatattcttt tttaaaatat gatactattt ctgacttcgt
    19801 cttatctaat aatgactctg gaactattcc tgccattgta agtgttctct tatagttggt
    19861 ttcattttcg ccttcacttt gaagccaaac ttcacaattt ttatttaatc caaaagcttc
    19921 ttttgacttt tcattcatat tcgcactagt acttacaact tcaccagttt gaactccatt
    19981 gtgttttaat cttgtcccta tataaactcc actacttaat acaaatactc caactagtat
    20041 taaggcagat attttccaag gaaataattc ttttttcttc ttttcaaaca ttgattacgc
    20101 ctcctttttc tattattttc tccaagctgt aagtttttat acaatttagg taaattttag
    20161 aagaaaatat atttatattt caaattactc taagaataga tattttctaa aaggtagttt
    20221 tctcttaata gttatattct tgtaattata tggacataat ttataataaa taatcgttta
    20281 ggaggtaata actttggaaa gaataaactg taatgtaatt aactgttcac ataacaacag
    20341 ccatatatgt tatgcagata aaatttttat aaatggtgaa aaagctcaca atgacagaca
    20401 tacttgttgt agttcatttt tagatgaatc tatttatagt aatttatcta gcaacgttaa
    20461 taatagaaat tgtgaagaat tagtttgcac tgtaaaaaat tgtaaataca ataaagatgg
    20521 tagcttatgt acacttgata atatattggt tgctcctgaa actgatagag taaaccttta
    20581 tattgaaaca tgttgctcta gtttcgaatg taaatagtca tagtataaat ataagggtgt
    20641 caaatgtaat taaactaaca tagtgtagag tatctacaac tattagtttg attatacttg
    20701 acacccttat ttattttaaa taaaatttca ttattataac catatttata atgttctaac
    20761 tgtaattaaa tttgataaat ttctataaat tggtataatc aatatgtaaa ttgaatttag
    20821 caaattaatc ataaaaataa aatatcgagt atatagtgtt atactaacta gaaatgaggt
    20881 gcttatgagt acattttata ttcaatttct ttattttgta atttatagct ttataggctg
    20941 gtgttgtgaa actacatatt gttcagtttt acaaaaacaa tttgtaaata gaggtttttt
    21001 aaatggtcca ttttgcccta tatatggatt tggagcatta gcaatagttg ctaccttgac
    21061 tccttttgta cacaatattc cgttgttatt tttgtttagt ataattataa ctagcataat
    21121 ggagtattgt actagcttta ttttagaaaa aatatttaat atgacttggt gggattattc
    21181 taaacataaa ttcaatattc acggaagagt ttgtctggaa aactcactta tgtttggtgt
    21241 attatcattg attgtaatgc ttatagtaca tcctattgtg gttgatttta ttaactctat
    21301 ctctaaaaat gtgctattta tatttgctat atctatagaa atttattttg ttttagattt
    21361 agtagttaca gtccatacaa ttttacaatt aaatggtaag ttaaaacaaa tcaatcttat
    21421 aattaaggaa ttaaaagata aaaaagaata ctacaaactt attacacaag aaactataga
    21481 aaacaaactt gaatctttag ttgaaaatag actggatact ttggaagata ggtttgaaat
    21541 acttaaagaa aaagaaaact atcgctatgc taaaaatcgt attgatgagc taaaaaataa
    21601 acttaatact ttactgtcta accacaaatt acttcataga cgtataatca aagcattccc
    21661 caacataagc tctaataagc atacagatat tttaaataaa atcaaagaaa atattaaatc
    21721 aattaaaaaa gataagtaat aaaataaaca cataagttaa ataataaaaa tactaataca
    21781 taagattaag gtttagaata ttttttgtaa aatactctaa accttaatta atatactatt
    21841 atttttaatc ttaatttata attttagttt attttacttt taactcaata aatttaagtg
    21901 tttaaacttc aatacactaa tatactaacc catcaatatc tcaactgatt ctctaagtgt
    21961 gctaatctct cccacatttc cagggaaaat tatatatggc atattaggaa atttgctttc
    22021 tttgcctgtc atccatactg gtataccagg ttttatctgt cccattactg tagctttttt
    22081 tactcttagt gctttagtac caacatcact agaagtaatt ccacccttag ctataataaa
    22141 atttggtctt acattaagca taccaattat actcgtaact gcatctgata tttctacaga
    22201 aatcataagc tgtttatctt tatcattcgt atccaggtca aaacgctctc gtctagtata
    22261 aacagcaaca gttttaccac tgcttatttt ttcctctaca acttttataa ctctttttac
    22321 ctcatttttt agaccatttt cttgaagaac taaatgttga ttaaattcta taaattctat
    22381 cggatattta caatttttaa gttcttctaa ctgttgggta gtcttattta catgtgaacc
    22441 aacaagtata attcctccat tagtattacc ttttgatact aactcatcct tagtaagcag
    22501 ttccttatca ctcactccgc caagtatttt tgttatggca gcagcgcttc taaacataaa
    22561 ttccttacca ctatttactg ctcgtataaa cgcaattgta aataccttta catctacata
    22621 gtcggttaca tttacaataa ttttattgaa tccttcagct tttttaagtt tttctgttat
    22681 agcttcaata tttagctctc taagttcttt tgacgaaata tatatcatat cactagattt
    22741 gtactttcct tttgtctttt cttcacacca ctcacctata tcagatgatt tatatccaaa
    22801 agatttatct tttgcaaatt cagtcattcc tgctggtaca agtatttctc cttctttaac
    22861 ataatgtaca ttatctatag taaacctacc accttcttta aaaaatggca ttattatttc
    22921 accattaaat aacttccctg ataatctctc tacttcgctt tttaataaat tagtttcaac
    22981 aggataatgt cctctcatag tagaatcact acgacttatg attataaaat ccttattaac
    23041 tttctttgat acatccacaa tattctttga aatttcttta tgtgcttttg tagtttgagc
    23101 aacagtaaat ccccttgaat tggtaagtat aaaaaacata ctatttttct cattaaaacc
    23161 ttgttccata ctatccttat cccaatcagt ataaacagaa atatcatgaa ctgtttgtac
    23221 acctgttggg tcatcatcta atacaacaat ttttctatct aaatgcttaa gctcatctgc
    23281 aagaagttca tttaatctat cctcatctac ttgtttaaaa gtattaagta tatctatact
    23341 aattggtttt acttctctca tttaaaacac ccctttatgt aatttgtata gtctaaatgt
    23401 aattttatat aatataaaca atagtttgtt atttatagac ctggaagtgg tataattcca
    23461 tttataacac taagcaataa aaccattata aatgctacaa ctccagcaat ggttcctcca
    23521 attgtccaag agtttaaagt atctttaact gataatccac taaatcttga aagtacccag
    23581 aatccagaat catttggaag ggagaaacca actcctccag cacatatagc aagccctact
    23641 aaaactggtg acattccagt tgccaaaatt gtaggtccta aaattgatga tgttgttact
    23701 aaggctacag tagcagaacc ttgagaaatt ctaagcaagg cacttaataa aaatccaagc
    23761 actacaactg atatactaag accagacata gtatcaacta agaagttacc tataccactt
    23821 gcatttataa cactgccaaa cgcaccacct gaacctgtta tcaataatat tagccctgct
    23881 gaatcagcag cttcaataac tacatctccc ataggtttat ttatgtattt ctttaaaaat
    23941 cctattgcca ccaatattcc aagaagtatt gctatgtttt tatctcctat aaatgcaagt
    24001 actccactca ttgtagaacc tttttctaag aacatactaa atacatttga tataagtata
    24061 agaagtattg ggaacaataa agaaaacata gaaattccaa aacctggtac tttagaagaa
    24121 tcatcatctt caatttttat gctatctttt ccttgttcta aatttttttc tctatcattc
    24181 tcatcaatag cataagctgg atatttttca aaacgttttc cataaatcca tccacctata
    24241 agagctgctg gaaaagcaac aattatagaa taaagtataa aactacctac atttgcattt
    24301 acatttgctg caactgccaa aggtcctggt gtgggtatta caagtgcatg acctactata
    24361 gtcccaactc ctaaggcaca tacaaaaacc attagagatt tcttagttac tctcgaaaca
    24421 tacttaatta taggcatcat tataacgaaa gctgcatcca taaaaactgg aattgaaatt
    24481 aaaaaacctg ctgtagttac tgctaaagca ctgttttttt ctccagtctt agctaacaac
    24541 ccctttgcaa tagattctgt tgctcttgat tctgaaagaa tatttccaaa tattattcca
    24601 agtccaatta caatacctat actccctaat gtatttccaa atccagtcga tattgtggtt
    24661 gagatttcat ttataggcat tctaaccata aagcctgtaa ttatagatgt tatcagtaat
    24721 gccaaaaacg gatttacttt aaatttaata atcgatacaa gtaaaattcc tatagatata
    24781 acaaaagcta tcaaaagtcc atatcctgta atcataatct ttaccccttt atcattctac
    24841 tttattattt atcaatctta tttttctttt actatgataa cttaacctcg ctgtatttat
    24901 ataatctaat cctaataata tgtatttaat tatattgata tatttgtata ttaattattg
    24961 tgttttttta tttcaacacc tgccaacttt tcaaaatact gtgctattcc actatggtca
    25021 tcttctaaat gtccatgtac ttttaatgtc tgcataattt ctaataattg acttgtcata
    25081 ggtagtggaa catctaaatt atgtgctgtt tgcattacat tcttaatatc cttcaaatta
    25141 atagatattt ttccacctgg tttaaaatca cgattcataa tcattggtat cttcgcatcc
    25201 aatattgtac taccagctag gcctcctctt attgccttat atactttttc tgggtctgca
    25261 cctgcttttg ctgcaagtac aaatgcctca gaaactgctg ctatcgtcaa attaactatt
    25321 acttggtttg taagttttgt aacacttcca ctaccgttat ctccaattaa aatagatgag
    25381 cttcccatta tataaaaaaa tggctctact ttcttataaa tttcttcctt accaccagcc
    25441 ataaaagcaa gtgtaccatc aatcgctttt ggctcccctc cacttactgg tgagtctata
    25501 aattctaagc ccatattttt taatttattt gcacaaaata tggattctgt aggagtaact
    25561 gaactcatat cgactactat acttccttct tttaaagttt ttgtcaatcc atccattcca
    25621 aataatatat cctgcacaat agttccattt ggaaggactg taaatacaat atcacattct
    25681 tgacccattt ctaaaacact tgcgtatttg gctccacatg atataagttc atccacggca
    25741 gatttattaa tatcatacac caaaagatta catccatcct ttaataaatt ttttgccatt
    25801 ggctttccca ttattcctaa tccaataaat cccaatttca ttttataacc ctccatatct
    25861 tcacattttt gaaaacgttt tttatgtttt agagtatact acataccagt ataccggtca
    25921 atataaaata taaaagtatt tattttaaca ataaatagta atttttaaag tcatttatta
    25981 tatttagtca tcctatataa gttttaatac atactattgt ttttaaatat aatatactac
    26041 ttttacagtt ttgtatttta actaatttca attaatttta attaatctgc ctttaatgtt
    26101 aatcatactt cacacttcaa taatttttta tattttttaa tattatctgc aatttaaatt
    26161 gcaaaagatt gaccttattt tcactatgtt gtatgcttaa aattatataa gtttttgtat
    26221 ttttatattg tttagggggg tattattaag tatggctaca tttaaaagat tacaagaaca
    26281 agcatatgac tacttaaaag aattgatact atctggagaa atgatagaaa atgaaatata
    26341 ttctgaaaca aaacttgcta gtgaaatagg tatctctaga acacctataa gagatgctct
    26401 tcaaagactt agtcaagatg gatttataga tataatccca agcaaaggat ttagaatcca
    26461 tcaaattaca gcaaatgaaa ttgtagagat attccaaata cgctctgcaa tagaagggtt
    26521 ttgtactttt ttaattacaa gtcaatataa ggaagctagg gcagtagaaa ctataagtaa
    26581 attaaaacat cttttagata aacaaaaaga tattttacta ggtgataaaa atttaaactc
    26641 atttgcagaa tacgatacat tatttcacac aactatagtt tcatatgctc aaaatactga
    26701 atttgacaaa atgtttaaca actatatgta cagaataaaa aaattagctc tcgattcatt
    26761 atcacatgaa ggaaggcttg aaacaacatt aaaagaacac acagatattt ttaataatat
    26821 tgcaaatgga tgtattgaag atatttacaa aactactctc atccatatgg aaactcctaa
    26881 atacattaat ttagaagatt tttgtaataa taagtgattt tagtattata ctttcattta
    26941 ttaaaaatat atctatctca aattaatttc ctaatttata aaaaatattc tataattata
    27001 ttgtaactaa tactattcta aggagtgatt tttttgaaca aagatgttgt aactaatgaa
    27061 actatcgaca aagattatgc tattgaagaa gttcgtatgg catgcaaaca ttttggtgac
    27121 ttatattttt atttttccaa agtgttattt gaagagtttg gtgaagataa aactagtgaa
    27181 atattaagaa aagttttatt tgaaaggtct gaagaaagag ctattgctat gagagaaaga
    27241 gctcatgaaa atggagatga attaattgca gacaatatta ttagcacaac tgatgttcca
    27301 tttctaggat gggttcctga atttgaagag ttacattgcc cttatggagc ttcctggctt
    27361 ttaagatttg atgaaaatcc ttggtttaaa aaattcgctt ctctttattg tgatgttaca
    27421 gatactactg tagctgaagt atttacagga gatacgtccc ataaaataac aaaaaatatt
    27481 ctatggggag ataaaagttg tgaaagaata tatttccatg atagcaaggt atctgaaggt
    27541 aaatatactt atggaaataa aaactgtaaa taaataattt aaattttcta tattcatata
    27601 ctatattttg tactttttca tataaatatt ataactagtc ttttatttta cttttgttta
    27661 acctaaagat aatttacttt taatctatat gaattatcat ataaataatc agaaagttat
    27721 ctttataaat tatagtttta gatttataat ttataaaata tttatgttct ttgtaaaatt
    27781 ttttagaagc ttctaatgtt atgctataaa actatcttga gaagggctga ttgaatgttt
    27841 aataaaatat atgaaaagaa agaatttata gtttttcaag ttaaaaaagg ttatgtagtt
    27901 tataatacta gaaagagttt tgaagaaggt catacacatt taaagcattt tgaagcagca
    27961 aagacagcta ttgatttagc aataaataaa aagattccta gaagcaaaga tggatattat
    28021 ctgactagtt taattcgcat aagtgatgat ggttattaca ttgataagtt aagcgaattg
    28081 ttatatgtta gagaacaaaa aggtaaaaaa gaaaagtact gtaattctgg ttatcagatg
    28141 taacaagact agttgtgaat tataagtacc aatcataagt acctttggtc ttaaaatcaa
    28201 agagtatcag taaatctata aaaaaacaac ttactgatac tctttttaat tatatgatta
    28261 atatttgaat ttgatacaaa attttataca aaagtttatg ttatctattc tgattctttg
    28321 tctgcaaatt ggctattata aagcttctca taaaatccac ccttttccat taattcatca
    28381 tgattcccct gctctataat acttccatta ttcataacta aaatcaagtc agcattgcga
    28441 attgtagaaa gtctatgtgc tataacaaaa ctagttctac cattcataag atttctcatt
    28501 gctttttgaa gcataagttc cagcctagta tcaacagaac tagtagcttc atcaagtatc
    28561 aatattgaag ggtctgatat aaaagctcta gctatggtta aaagctgttt ttctcctaaa
    28621 gatatattag atgcttcttc atttaagaac atattgtatc catctggaag agtggttata
    28681 aagtgatgaa cattggctac ttttgctgct tgtataattt cttctttagt agctccaaat
    28741 ctaccatatt ctatattttc aaaaatggtt ccattaaata accatgtatc ttgaagcacc
    28801 ataccaaaca ttgaacgcaa atctttacgt ttcatatctc ttatatctac tccatcaatt
    28861 ttaatagcac ctttctttac atcataaaac ctcatcaata ggtttattaa tgttgtttta
    28921 cctgctccgg taggtccaac aatagctacc atctgaccac tttttacttc tgcatttaaa
    28981 tcttctatta aagttgaatt ttctccgtat ccaaaattaa catgctcaaa tgtaacattt
    29041 ccttttacat tttctatctt aactggattt tcaacatcag gtatttcctc ttcttcgtct
    29101 aaaatttcaa atacacgttc tatagctgca aaagaagatt gtattactgt ggataactga
    29161 gtcatttgtg aaagtggctg atttatctgc catatataac gcacaaatgc ttgtaaattt
    29221 cctactgtta tccctccact tactgcatat atagaaccga caactgccac tgctgcaatt
    29281 ccaaagtaag ttactaatga aaccatagga ttcatcatac ttgatataaa ttgtgcctta
    29341 aatccatttt cacaaagttc ttgatttacc tttttaaatt cttttatgga atcttcttct
    29401 ttaccatata gttttatttc attaaatcca gtatacattt cttgtacttt tccatttaat
    29461 tttccaagag cattttgttg caatgaaaat ctactttgag atttttttac tacaaattta
    29521 gatacaaaat aactaattgg aattataaaa agagctataa gagccatata tatattaatt
    29581 gtaaacatca ttgaaagtgc caaaattatg actaaaattg agtttacaat ttgcataaaa
    29641 ctctgttgca atgcatttga tattgtatct acatcatttg atatacgact taatacatcc
    29701 ccaaaactat tactatcaaa ataactaata ggcaatcttc ttattttatt ttctacttca
    29761 tttcttaaat ctctcattgt gttttgaata gcattcgtaa gtagaaaact tgaagtatac
    29821 gtaaaaattg cattggcaaa atatatacat gctaaaatca ccaatatttt tgttacatag
    29881 tcaaaattta cggacgcccc tggtactcct tttgcaatac taattacatc tttagtcaat
    29941 tgtgttatta tcaatccttc agttttaggt gcaagtgcat tcatagtagc tgctgaaatt
    30001 atacatataa tagctataat aaaggaaaat ttatatttct ttacaaatgg aagaagtcta
    30061 ccaattgttt ttttaaaacc tttagacttt ttcattattc taattcctcc ttttttaact
    30121 gagatgttgc aatttcatag tatatttcac agttttttaa taattcttta tgagttccca
    30181 ttccgacaac ttgcccttca ttgagtacta taatcttatc agcatcaata atagaactaa
    30241 ttctctgggc aacaatcaat acaattgcat cttttgtttc cttcttcaat ttagcacgaa
    30301 gtattgcatc agttttaaag tcaagtgcag aaaaactatc atcaaaaatg tatatttcag
    30361 gtcttcttat aatagccctt gcaatagaaa gcctttgttt ttgacctcct gatacatttg
    30421 ctccaccttc actaatcaat tcatcaaact ttctaggttt ttttgatata aactcatatg
    30481 cttgtgcaac ttttgcggaa tattctaatt ctgactctcc tgccttatgt ttcccaaatc
    30541 taatattatt tgctatgcta ccagtaaaaa gcagtgattt ttgaggtatg aatcctatct
    30601 tttttcttaa agcttttaaa tcatattctc ttatatctac tccatttatc ttaatgctac
    30661 cttctgttac atcgtaaaat ctaggtatca agttaattag agtacttttc ccactaccag
    30721 tacttcctat aaaagcaact gtttcacctg tttttgcagt aaatgaaata tccttaagta
    30781 cagaagcttc tccatctgga taggtaaaag ttacattatc aaattctatg atacccttat
    30841 tttcagtatc cttaattcca ttttcaggat tttttatcaa tggttcttca tttaaaagtt
    30901 cttttattct atttgcagat acctctgctc ttggatacat aataaatacc attgaaaaaa
    30961 gcataattga aaacatggca tggaaaagat attccaaaaa tgcaaccaac tgtccaactt
    31021 gaagttctcc aatatttatc ttctcacttg aaatccaaaa tacagccaaa acagctatgt
    31081 ttaatagtaa aaagaatgct ggttgagtaa ttgacataag cttaaataat ttcttagaaa
    31141 cattagcata atatgtattt gttttttcaa atcgctcggt ttcataatca tcatttccaa
    31201 aagctctaat tactcttatt ccagttaaat tttctctcga tataaggttt aatttatcta
    31261 gtcctttttg ttgttttgaa gatattgcct gagatacttt tgctattatt gctactccaa
    31321 taattataaa aggtactgat atagccaata ctatagaaag ttccacactt gttctaatgg
    31381 tcattattat acttatgatg aacataactg gagttaataa ggcagtcctt agtagtatat
    31441 taacaaattg ttgaatttga aatgcatcat tcgttgttct tgtaatcatt gaagaaattc
    31501 caaatttatt gtattcagta tgtgaaaatt cttgtgattt ctcaaatata tcatttctta
    31561 tatctctggt tacacttgtt gataccttag cagaacaata acccagcaat atactaccta
    31621 caacccctat aatagaaata ataacaatca ctattcccat ttgttttatg taagctatat
    31681 tctgatttgc aataccattg tcaattattt tagctattat agttggaatc cccaactcga
    31741 ctaatgcaaa actaaatata gccagtacat ttaatactat taataactta taattcttta
    31801 aataacttaa aattaatttc atatttttct cctttcttaa tttttgagat agtgtaatca
    31861 tgtaataata ctaaatttat aaatgcttaa ttttataaaa aaaggagaaa agagttaacg
    31921 cactctcttc tccagcatat tctttttaat acactctccg acaagtcgct tatatgttaa
    31981 catatttgac aagtaatgtc aattcatata gccaaaagtc ttttaaatgc tccatttcat
    32041 gacattgtaa caaattggca tgtaatttat ttttgttttt tactgtattc caattttaca
    32101 agttctccaa tattactagc tttcttaaag ttcaaattaa tcttgtctat accagactta
    32161 aatagtgaaa tccctctccc caatattatt ggcaatatat atatataata atcatcaatt
    32221 aagttatctt tcataaataa ttttactata tcgcctcctc caactatcca aatatctttt
    32281 ttctccttct cttttccaat ttgctcaatt aattttttag gttctaaatt agtatacttt
    32341 acattctcag ttgccccatt aattgtcttt gagtatacat agcattcctt acccttatag
    32401 taccattcat ttgggcttaa ttcatttgca atctgtacat atgtggaatt tcccataatg
    32461 atagtatcaa ttgtatcgta aaaagtatta taatcatctt cctgctcatt cttaaattct
    32521 gaagatatct cgtccaaaaa acttacactg ccattctcct cagctatata accatctaaa
    32581 cttgtggcaa tataaagtac tacttttctc atgtttacta cctccaacat ttatagtgtg
    32641 attattctat gcaaacatca cttttttact cattcaataa taccctatta ataaatcctc
    32701 aaactaattt aaataaactt tgcataaaat taaaaatata ttatataaat aatttaaaac
    32761 cacaataact tattgaaaat tctaattatt acaaataaat attgctctct gttaacgcta
    32821 gttttataat ataattataa gctattaata ttataaagcc gatattacta atataataat
    32881 tataatactt atagttaatt tacttttgtt gaaaggtggt aaataacatg aaaatttcag
    32941 aagtcgctac tccaaatttt ttgctagatt tagaccagtt agaaaagaat atagataaaa
    33001 ttcaacaaat ttgtacaaat aataataaac aactttggcc tatgcttaag acacataaaa
    33061 gtacttacat agcgagactt caaaaaaatg caggtgctag tggttttcta gttggaacac
    33121 ttgatgaagg tgaagctttg ataaaagcag gttttgaaga tattatgttg gcttatcctt
    33181 acatgggaga ggtcaatata aatagaattg tgactatggc atcaaaaata aatttaattt
    33241 gtgctacaga taatatagaa tgtgctaagg cttatagtga agctttttct aaatcaaaaa
    33301 tagagtgcaa gctacttatt attgtagatt gtggtttaca ccgttttggg gttgaaccag
    33361 aaaaagtggt tgaacttgct actgaaattt ctaagctaga aaacatcaaa attgttggta
    33421 taagttcaca tccaggtcaa gtgtatggtt gtagttctcc agaaggtgtt ccagatgttt
    33481 gtgttcaaga agattcttct atggaaaaag catacaaagc tctaactgat aatggattta
    33541 atatagaaat tgtcgcttct ggtagtactc caacagttga atctgaagct gcttctaaaa
    33601 ctataactgc tgtaagacct ggaaactatg tatactatga tgctatacag ataggtttag
    33661 gttgtgctac tgaagatatg tgtgctctta ctgttgtgtg cactgtaatc tcaaaaaata
    33721 gcagtggata ctatttaatt gattgtggaa gtaagtgcct tggacttgat aaaggtgcac
    33781 atggaaatag tagtattgtt ggttatggta gggtatgtgg acatccagaa cttactattg
    33841 attcactttc agaagaagtt ggaaaaatta aagtaaatgg tgaaactgat ataaaaattg
    33901 gtgatagaat aagaattata cctaatcatt cttgttccac tgcaaacttg acaagtaatt
    33961 tattaggttt tagaggagat acaatagaaa aagtcataga agtagatatt cgtgaaaatt
    34021 caaaacaaat catcctttaa aaacagattt attctgatat taacatgaaa gtatattttt
    34081 cttaaaatta ttgttaataa ctaatgttaa tgttggataa ataaaaaaca taaattctag
    34141 aaatataaat ttaaaagaag aggctgtctc aaaatagaga taagcctctc tttcattgtt
    34201 gaaattatga aaatatactt gatttcatac aaaaagagta tctcatgaac taaaaagttc
    34261 actttgagat actctctttt ttttaattaa tatacacatt aattactatc ttagtttatt
    34321 actatatatc aattactatt cattaatcct tgtatatatt aattcttata tatcatttat
    34381 agttaggtta taattttatg ttatttacat taatcccata acagtgagta aaactcttac
    34441 tattaacatc cccactattg catcaaatat aggaactgcc aacagtaaca ttctatactt
    34501 agtactggca tttgctacaa gcactattct ggcataatga ccaactagag tacccattaa
    34561 catacatggc ataattaata tggttgcttg tggtgcagtg attagacctt gtgcaaataa
    34621 gtttgcagct gtagctgctc ctgctgcctt agcaaagaat gctgctatca aaactacaac
    34681 agattcccca ggtagtccaa aaatacccat tactggtcca aaaatatttc ctaaaatatc
    34741 aactaaacca gttaattgta aaaactgcac tattacataa cccattatca tggctggtaa
    34801 tatctgttca acacctatgt aaaaaccttt ttttgctccc cccataaata tctcaacagc
    34861 agttttctta ggtttactat ttacaattgc atttccagta ttttgttgac tcatactaca
    34921 cagcctcctt agccttattc tttttatttc ttacacctat gattaatcta actagattag
    34981 caccaagtat tttacagaat atctctataa ggattattgg acctaatgcc aataatgcta
    35041 ttggtaaaag tggagcttga gtatttattg tgttcagtat aacagctgaa ccagcatatt
    35101 gatatgctac aaatatagtt ctttcatcat ctgttatttc cccactttca aataattctt
    35161 tcgtcattat tgaggctaca tctgaactag taaatgaaga tacaaatgct attcctgcta
    35221 ttccaggtat accaagcaga ggtctaagta atggattaaa tatctttgat gctgctttca
    35281 ttcctcccat agattcaact acatctataa gacctacagc aaaacagaca gttggaatca
    35341 aagtcagagc aaataagaaa ccatcttttg ctcccgttcc tcctttaccc ataaagtcta
    35401 caccatctgc aatagtacca aatgaacctg tcagattagc aaaatctaat gctttaagga
    35461 agccttgact atctttaaac actcctgaaa ataatactat caatacaaat aaagataaat
    35521 accctttcca tgttacctta ttatctccag ctgttacatc catataaaat ccccctttat
    35581 acctcaaatt tatctaagtt taaaataact gtagaaatta atttcaccct ctcaaacata
    35641 gaatctacta ctgcatattc tcttgatgtg tggttccatt cccctcttac accaaatgaa
    35701 cagattgtag gaacgtttgc aatagttaaa taacttgcat ctgaactacc tccaagtctc
    35761 ttagcattta attctccaaa tccattttct tttgacactt catttacaaa tgtatgaaat
    35821 ctcataacat cttctgtagt ttcaaatggc atcatttcac ttataaaatt aacttctgta
    35881 gaagttcctt ctatgtaagt ttctttacat atctctctaa cttgttcttt aacattctcc
    35941 atttcttcta ctttttcaaa tctaaggtct acttttatac tacaatcttc tggaatagaa
    36001 ttttccactc ttccaccctt aatcacactt acacttactg tagttccaac atctaaatta
    36061 gtcaattttt gtatgcgtag tatcttatta gccatttctt ctatagcatt tcttccacct
    36121 tcaaaatcat tacctgcatg tgtttctaca ccttttacag ctatattaca tcctattctt
    36181 ccttttctac ctatacataa actattatct actaatccag tttccatatt aaaagcacaa
    36241 agaccttctt ttgcttccct caatataaca tctgcacctg ttgaatcctt atgtcctatc
    36301 tcttcatctc cagaaaatac tatttttatt ggtctttctt tgtatcctat tttatttaat
    36361 gctttaataa catataagga aattattata ccacccttca tatctaaaac acctgggcca
    36421 tatgcttttc cttctattat tttaaatgga ttctctccaa atgtacctgt ttcaaagaca
    36481 gtatccatgt gacctgaaaa aattattggc tttttatcta tattagaacc aagtgtacct
    36541 atcaatgtac taccattatc tccaacatct actaactcgc aatctaaacc ttctttttca
    36601 aactcaaact ttaatctctt agccaattta tttacgcttt ctttacaatg tgtatagctt
    36661 tccatattaa caatctcttt ccataatgaa actatttctt ctctattctc atcaacaaac
    36721 ttagaaattt catttaaata tgtattcata atcttctcct ttattttcaa ataaaatgta
    36781 tttctttcct aaaacctaag tttattttag gaaatcttaa aagactagtc cttaattaca
    36841 aaatagcaca attattattg ccagtcaaca tatttttcag tcttttttat attttctgat
    36901 ttttctttca attatccttt tgtaaaataa attgacgcgt tttatctact attataaaat
    36961 gaggttataa attgattgat tagttacaat actctcgaaa ttttaaagga ggttttatga
    37021 aatataaaat tggttttttg acaacatctt attttatgtc agataagttt aaacaagctc
    37081 ttaaagaatt aaatgatact tgtgaaatta catttattga gcttaaaggg gagaatcctt
    37141 ttaataattt acctaatata tactatgagc aagtagataa ttttgatgga tttgtatgca
    37201 gtggtatagt tccatatagt gaacttataa taaatataaa agacataaaa gttccattaa
    37261 attttttaaa gcttgatgaa agagattttt ataaatactt attcaaatta ttaaatagcc
    37321 aaaaagatat tgactttaca aaatcattta tggatttttt aagagaggac aacaattatt
    37381 atgatatata ttcattaata gactctaata aatgtccata tacaattaaa gattttaata
    37441 tccctcaacc tgtatatgac tttaaaaaat taaatgataa attattggaa atacatttag
    37501 acttattgcg agataataag attaaccttt cttttactag aaattacatc attagttgtg
    37561 aacttaataa acttggatat gattgtattt acatggttcc atctacagaa tcaatcctga
    37621 atacatttaa atctctggtg aatgaaataa cattacaaaa cttagacaaa aataaatctg
    37681 caacttgtat agttacagta aatagttcag agtacataaa tgaagatttg atatttaaaa
    37741 atgatgagat acaaaatcaa atatataaca ctatattaaa ttctctatca agacatggat
    37801 tttatggagt tcaggtcaaa aaaaatgata tgaaaataga gatacacact actaaagaaa
    37861 tgttaaacaa tatgaccaat aactatacag atttttttat ttcagaagat ttaaaagaaa
    37921 ttaaatatag cttaaatata ggttggggca ttggaaatac aaatattcat gcagagcaaa
    37981 atgctcgtca agctaatggt aaatctgctt ctttgaatgg aaactgtact tttatagtaa
    38041 atgatactaa tgatacaata ggccctttat attcaaataa aaattctaat aatattgaag
    38101 atagctcaat agccaataaa gttgcttctt ttatacctct atctaataca aatgtatcta
    38161 aaatcatgtg tatgattaat gatagaaatt caaataatgt gtcagcagaa atattagcag
    38221 actatatgaa cataactctt agaagtgcta atagaatatt gagtatttta tataaagcag
    38281 gaattgcaac tattgtaaat acaaaactcg ataatcaaag aggaaggcca aaaaaaatat
    38341 ataaagttga tttcttatct tttctaaata agatgcagaa attaaattct taatatttaa
    38401 ttttttaata aaagcaatgt attttctcta attttaaaga atcaaaatgt ttaaatttta
    38461 ttagaatata caacttttta ctttaaaata cctaaacctt ttatcagtga aataaaccat
    38521 aatatatcgt aacttattag aaaaacaaaa taatttcata tacaaataat aaaatagcca
    38581 ctacaatcca taatttgcag tggctacttt ttacactatc acttattact tatttttaaa
    38641 aattaattgt attttttaaa taatttatac acacctatta tcagtaaagc aataaataca
    38701 agtatatcta taaaaataat tattgtaaca tgtctggcaa tattgtaaat aaaaagtata
    38761 gagaagattt ctatagacaa cgctacaaaa cacataaaat tcgttaaata atctgcaata
    38821 gtatcactat aaaaagcgtg tatttgtaac catttccata taaatggacg taaaaggatt
    38881 ctgaatacaa cacaaatgac agggtaggca taaataaatt ttttatctac ataattagct
    38941 acaccattat tccattggat aggaatttga ggtggcaact ttggaaatac tgttattgca
    39001 atttaaacac tacctctcta ttctattaat ttataactat aatatagtct taaata
//
LOCUS       DAEORZ010000024        37753 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEORZ010000024 DAEORZ010000000
VERSION     DAEORZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.024"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..848)
                     /locus_tag="KRH39_001072"
     CDS             complement(<1..848)
                     /locus_tag="KRH39_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8510992.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENGKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLA"
     gene            967..2166
                     /locus_tag="KRH39_001073"
     CDS             967..2166
                     /locus_tag="KRH39_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBG8510993.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(2360..4915)
                     /locus_tag="KRH39_001074"
     CDS             complement(2360..4915)
                     /locus_tag="KRH39_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG8510994.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     VGLSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFSLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINAPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKS"
     gene            complement(4912..5598)
                     /locus_tag="KRH39_001075"
     CDS             complement(4912..5598)
                     /locus_tag="KRH39_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8510995.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            complement(5675..6583)
                     /locus_tag="KRH39_001076"
     CDS             complement(5675..6583)
                     /locus_tag="KRH39_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8510996.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(6585..7256)
                     /locus_tag="KRH39_001077"
     CDS             complement(6585..7256)
                     /locus_tag="KRH39_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8510997.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            7518..8582
                     /locus_tag="KRH39_001078"
     CDS             7518..8582
                     /locus_tag="KRH39_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBG8510998.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            8740..9426
                     /locus_tag="KRH39_001079"
     CDS             8740..9426
                     /locus_tag="KRH39_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8510999.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKGNYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKVKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            9416..10447
                     /locus_tag="KRH39_001080"
     CDS             9416..10447
                     /locus_tag="KRH39_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8511000.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITGDLKLIALLL
                     IYTMVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMIKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            10529..11194
                     /locus_tag="KRH39_001081"
     CDS             10529..11194
                     /locus_tag="KRH39_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8511001.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            11207..13624
                     /locus_tag="KRH39_001082"
     CDS             11207..13624
                     /locus_tag="KRH39_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8511002.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDELRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(13805..14596)
                     /locus_tag="KRH39_001083"
     CDS             complement(13805..14596)
                     /locus_tag="KRH39_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG8511003.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(14629..15216)
                     /locus_tag="KRH39_001084"
     CDS             complement(14629..15216)
                     /locus_tag="KRH39_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8511004.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKSDWNLSI"
     gene            complement(15393..16784)
                     /locus_tag="KRH39_001085"
     CDS             complement(15393..16784)
                     /locus_tag="KRH39_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBG8511005.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(16968..18272)
                     /locus_tag="KRH39_001086"
     CDS             complement(16968..18272)
                     /locus_tag="KRH39_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBG8511006.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNNYY"
     gene            complement(18629..19504)
                     /locus_tag="KRH39_001087"
     CDS             complement(18629..19504)
                     /locus_tag="KRH39_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBG8511007.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(19697..20383)
                     /locus_tag="KRH39_001088"
     CDS             complement(19697..20383)
                     /locus_tag="KRH39_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBG8511008.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(20432..20965)
                     /locus_tag="KRH39_001089"
     CDS             complement(20432..20965)
                     /locus_tag="KRH39_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG8511009.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLAILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(21009..21641)
                     /locus_tag="KRH39_001090"
     CDS             complement(21009..21641)
                     /locus_tag="KRH39_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBG8511010.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(21731..22810)
                     /locus_tag="KRH39_001091"
     CDS             complement(21731..22810)
                     /locus_tag="KRH39_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG8511011.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(22943..24289)
                     /gene="pepV"
                     /locus_tag="KRH39_001092"
     CDS             complement(22943..24289)
                     /gene="pepV"
                     /locus_tag="KRH39_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBG8511012.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(24358..25638)
                     /locus_tag="KRH39_001093"
     CDS             complement(24358..25638)
                     /locus_tag="KRH39_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBG8511013.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(25765..26025)
                     /gene="hfq"
                     /locus_tag="KRH39_001094"
     CDS             complement(25765..26025)
                     /gene="hfq"
                     /locus_tag="KRH39_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBG8511014.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(26139..27074)
                     /gene="miaA"
                     /locus_tag="KRH39_001095"
     CDS             complement(26139..27074)
                     /gene="miaA"
                     /locus_tag="KRH39_001095"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBG8511015.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDINAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(27112..29079)
                     /gene="mutL"
                     /locus_tag="KRH39_001096"
     CDS             complement(27112..29079)
                     /gene="mutL"
                     /locus_tag="KRH39_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBG8511016.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKKSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYHLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(29090..31933)
                     /gene="mutS"
                     /locus_tag="KRH39_001097"
     CDS             complement(29090..31933)
                     /gene="mutS"
                     /locus_tag="KRH39_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBG8511017.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(32093..32827)
                     /locus_tag="KRH39_001098"
     CDS             complement(32093..32827)
                     /locus_tag="KRH39_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8511018.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFIS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(32796..33809)
                     /locus_tag="KRH39_001099"
     CDS             complement(32796..33809)
                     /locus_tag="KRH39_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8511019.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(33831..34601)
                     /locus_tag="KRH39_001100"
     CDS             complement(33831..34601)
                     /locus_tag="KRH39_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8511020.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(34576..34866)
                     /locus_tag="KRH39_001101"
     CDS             complement(34576..34866)
                     /locus_tag="KRH39_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBG8511021.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(34997..35096)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      35716..35805
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            35858..36034
                     /locus_tag="KRH39_001102"
     CDS             35858..36034
                     /locus_tag="KRH39_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511022.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(36149..36505)
                     /locus_tag="KRH39_001103"
     CDS             complement(36149..36505)
                     /locus_tag="KRH39_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8511023.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLEIDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            36761..37673
                     /locus_tag="KRH39_001104"
                     /pseudo
     CDS             36761..37673
                     /locus_tag="KRH39_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816618.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
BASE COUNT        12815 a         5930 c         4365 g        14643 t
ORIGIN      
        1 gctaaggata aagatatatc ttttaaatgg cagtaaccta tatttctctt aggtattggc
       61 tcttttatat ctaatttaaa cagtttccca ttttccaaat aatctatgct aaattcttct
      121 ataacacaag acactcctaa atttatatac gcaaattcta aaagcaattc atgagaaccc
      181 aattctatat ccgggtttag ttttatacct tttgataaaa aatattcatt tacataaagt
      241 cttgaatttg ctttattttc tagcattatt aaaggcaatg tatttaattc ttctaatgat
      301 attttcctac ctttgtattc tatgtaatca tttgcacata caaatatatc atgtatttca
      361 atacactcaa caatatttag tgtatcatct tctattggca tgtttataat tgctatatct
      421 atattcccat ctttgagtaa agatataagt tctctactcg ttctatttat tgtagataca
      481 tgtatatgtg gatacttctt atgaaaaatt tctaaatatt ttaacaagaa aaaacgtgca
      541 gctgtatctc ctactcctat tttcagagaa ccgtattcta attttttaaa tttatttatt
      601 ctatcttctc ctgatgatat taactttaat gcagtagtga catgttcact tagaattttc
      661 ccctctggag ttaattttac tcccttattg tttctataaa atagtaaagt atctaattgt
      721 tcttctaatt gctttattga ctggcttaca gctggctgtg acataaacaa ttccttagca
      781 gcaagagaaa aactcttatt atttacaact gcattaaaaa ccttataaag ttcaagtttt
      841 atattcatat ataattactc cttatgtcaa atattagtat tatttatttt acttataatt
      901 atatttgaat tataatatac atggtactaa tatgacaata ttaattcttt aggaggattt
      961 tagataatgg atagattagt tggaactgtt tcaagagggg ttagagcacc tattataaga
     1021 caaggagatg acttagttaa aatagttgtt gattctgttt taaatgcgtc aaaaagtgaa
     1081 aactttgaag ttagggacaa agatgtaatt gccgttacag aagctgttgt agctagagca
     1141 cagggaaatt atgctcacgt tgacaatatt gcaaaagatg taaaagataa atttggagat
     1201 gatactgttg gggttatatt cccaatttta agtagaaatc gttttgctat atgtttaaaa
     1261 ggaattgcaa aaggatgtag aaaagtcgta ttgatgttaa gctacccttc tgatgaagtt
     1321 ggaaatcact taataagtat tgatgaactt gatgataagg gaataaatcc ttggtcagat
     1381 gttttaacag aagaaaaata cagagaacta tttggatata acaagcatac atttactggt
     1441 gtagattatg ttgattatta taagaattta atcaaggatt gtggtgcaga ggtagaaata
     1501 gtatttgcaa acaatccaaa aactattctt aactatacaa caagtatttt aacttgtgat
     1561 atacatacaa gacaaagaac aaagagaatt ttaagacaaa atggagctaa aaaagtatat
     1621 tcacttgatg atataatgac agcaagtgta gatggtagtg gttacaatga ccagtatgga
     1681 cttttaggtt caaacaaagc tacagaagaa acagtaaaat tatttcctat aaattgtgat
     1741 gaagttgtaa acaaaatcca aggtaatata aaagaaatca cagggaaaga tgtagaagta
     1801 atggtttatg gagatggagc ttttaaagac cctgtaggta aaatatggga attagctgac
     1861 ccagtagtat ctcctgcata tacaaaagga ttagaaggaa ctccaaacga agtaaaatta
     1921 aaatacttag cagataatga ttttgctgac ttatctggtg atgagttaaa agaagcaatt
     1981 tcaaaataca tagttgaaaa agataataaa tctgatgatt taacaggaaa catggtttct
     2041 caaggtacta ctccaagaag attaacagac cttataggtt ctttagcaga tttaacatct
     2101 ggaagtggag ataagggtac tcctattatt tacatacaag gatatttcga taactataca
     2161 aaatagtatg ttttaataag atttaaattt tttggattat atatattagc atgaaaattt
     2221 attaaaacct cttaatagat atgacttttc aagagttata tctattaaga ggtttattta
     2281 atgttcacag ataagatata tttaaataat ttaaacaaga ttgtcagaaa tttatataat
     2341 cttaaaaatt tatataactc taactcttat ttatttctga aataatatca ctcttcaaaa
     2401 tttgtcttac tggaataatt acagcaacta tactaatagt gatatttaaa ataatcatac
     2461 ctatgtatag aggtgcattt attttaagaa ttggatttat atatagatat acacgtttta
     2521 ccataaaata aatcataaat tgtccaataa cacttccaat taccattata atacttgcat
     2581 agaatccata taaaaaccct tctcttatca tcataagtga aaacttatta tctgtaatac
     2641 ccatagctct taaaattcca aactcatgtc ttcttgaaaa aatcaagtaa cttactgtat
     2701 tgattatatg gaataaactt attgcaagaa gtacaaatac aataccatat ataaataaga
     2761 gtttttgatt taagtaggta ttattttttt caatagcaat agtataatct gttacgatac
     2821 atctttttac atcacttaca acacttttta tgttctcaga taatcccaca ctattactat
     2881 cttctgtctt ttttatactt actgaattat aattttcgat atcaaagtta tttttcattt
     2941 gcccattcgt cataactata tccataccat aatcaccaat gaaatatatg ttatttgcca
     3001 tcacacgttt tacagtagca gcaactacaa attcttttgt tgtataatca ttcttatttt
     3061 cttctttaaa tttaacaatt tcatctgttg gtttcattgt ctttggcact ttaactttta
     3121 ttgtatctcc tggatttata tcaactgcat catacatacc agttccatcc ataggtagtc
     3181 taactataac cttattattt ttaaccatat catgggtatt aatctcccca tctataatat
     3241 aagaatttaa tccttctaac ataccattgt tataaccata aatatttgtt tttaaaagat
     3301 agccaccttc attttgcttt gtacaaatac catcaaaaaa agtctttatc ctgtaatctt
     3361 tttcaagagg tttaaagaaa tttttccaaa atagttgttc ttctttaatt tgcatagctc
     3421 caaataaata tcttactgga tagacttttg atattccctc aacattgttt aatcttgaaa
     3481 ttttatcttt tggtatacct acatcaaaat ctgttgtttg catagttatt tggtagtcag
     3541 aatttaaacc atcatcagct ttcattgtaa gttcattatt atttttagta agctgtatag
     3601 agtaactaga acataaaaat ataattccac caagtgataa tgaaaacaaa attccaataa
     3661 acattccctt tttttgagtc atgtatttat gagataatac ttttgtcatg ttagagtgtt
     3721 ttacactttc taatattctc tttttcttat tagtaatctt aaagtttttc atagattcta
     3781 tactagagta tttattcaac tgatgtacaa cacgtacagc aattaaaatt aacaacaata
     3841 agataaataa aaagcctaaa taaatagatt tttcacttat aaaaaatgct cctggaacaa
     3901 tatcactact caaaaatata tgtggaaatt gttcatatat tgtagacgct actccaatac
     3961 ctaagaagca acctatagga aatccaacaa taaaaagtaa aaataactct aaaaatagta
     4021 gaaataaaag ttgtttatta ttagcgccta atacttctaa aactccatat tgtgatattc
     4081 tctgaagtat tgaaacatgg aaaatactat atactgctgc tgcaccaaac actccaacaa
     4141 gcaatataat tgcatttgta tttagttcta aattttttaa gaactcaaat atattatatt
     4201 tttctatatt ttttggtggt tttactccaa attcaactga aagaggccag tttatataag
     4261 ttttttcttt tgatatgttg aattttttca taaatttata attttgcttt ttaatatttt
     4321 tattttcatc atattttaca taaactatat aagaatcctt ttctttaggc tgaaaataat
     4381 cgtttacaaa tccctccaga gcagtttctg ggtttgaaag acttgataaa ataccacata
     4441 acttatagat tttaccatct aaattgattt ctgttccaat ttctttgttt acatttaaat
     4501 tgtttagaga atatgtgtca agtactatct ctccatcttt actaggaaga gttcctttta
     4561 ataattttct acctgtcatg tctaaatatg aagaatcagc atttaaaaat tctataataa
     4621 aaggttcatc tatctgattt ttaggttttg taacgccaag tctatctaca gtgtattcct
     4681 ttgatttttt atttttttta attttattaa gttgttcctt atctacttta tagaaataat
     4741 gataatctcc actatcttcc cttatctttt caattctact tttgtctgca ctatgaacta
     4801 aagaaccaat tcccgtcaat aatgctactg atagtactat acttaaaact atagatatag
     4861 attgtttttt ataatgcttt atatattgaa ttgaaaattt tactagagac atcatagtac
     4921 ttcaccacca gtattcacca caactccatc ttcaatatgg ataattgagt cgcatacttg
     4981 tgctatattt tcattgtgtg ttaccataag tatcgtttga tggaattttg cactactttc
     5041 ttttaataaa catactactt ccattgtagt tttactatcg agatttcctg ttggttcatc
     5101 tgctaatata agcgcaggtt tatttgccaa agctctagca attgctaccc tctgttgttg
     5161 accaccagat agttcgcttg gaaattttaa acgcttatcc caaattccca atgtcttcat
     5221 cagcatttca atatattcat ggtctatatg gtttccttta tctaatgtaa caggaagagc
     5281 tatattatca taagtgttta atacgggcat taaactgtag ttttgaaata taaatcctat
     5341 atttcttctt ctaaatactg tcaaatcttt ttttcctatt ttagatatgt tctttccttt
     5401 aattattaca tcacctgaag ttggattatc taatccacct ataatattta acaatgtgct
     5461 ttttccagaa ccagaagttc caactatagc agtaaatgta tctcgttcaa tttctatact
     5521 tactccattt aaggctttta ctttagtctc accttttcca tagtgtttta ctaaattatt
     5581 cgtcactaat atactcattt aatttctcct ttcaatacag atatatgttc tataaaatat
     5641 tgtaagtgac gataattaca ctcatcctac aaaattagtt taaactctta caattctgta
     5701 agaagatact aaaacaacat ccacttccta gtttagattc tactataatg tttccatttt
     5761 ctttttctaa aattaatctt gtaagatata gaccaatccc tgtgccattt tggttttcca
     5821 catttttact tctgtaaaat cttttaaaaa tatcatttag ttctctacta tcaataccta
     5881 ttccctcatc tttaatagta atcttcgtat atagttccat ttttattaca gaaatagtaa
     5941 tcgtgctatc tgatggtgag tattttattg cattttctaa aacatttaca attgcttcta
     6001 tggtccactt tttattatga actaatttta tgtctgaaaa tggttcgaga tttatcctta
     6061 tatttttagc ttctgcttta ttaaagatag tactgatact ttgaataaga gtatctttaa
     6121 ttagtagttc ttctacttca aattctataa ctccatcctc taatttagtc attttaaata
     6181 aggattgtaa caaccagtca atcttaaaag tttcattttt catcttttgt aaaaactcct
     6241 tcttctgtaa tgttgaaata tttatattct ccagaagttc acaataaatg gttatatttg
     6301 aaagtggagt ttttaattga tgtgacatat ttgatattaa acctttaatc gcttcttttt
     6361 ctgactttga ctgttctacc tctatttcta tcatatcctt aatttttttt acttggtgag
     6421 aaatttttga cgctcttgta tccttcgtat ctggataagt aacttcttta ccttccaata
     6481 tttgatttaa catcaaattt aattcatcat atcttctttg atttatttta gtagatacta
     6541 taaatataac aactgtacat attataaagg ctaatataaa catattattc tacctcattc
     6601 cataaataac ccattccata agctgttttt atatattttg gattagatgg gtcatcctct
     6661 acttttactc taagtcttct tatatttaca gatacaatat tatcatcaac ataattttct
     6721 tcgctgtccc aaatatggtg taaaatttgt tcctttaata atatttgacc tttattttca
     6781 attagataac ttagaagctt atattctaca gagctaagtt ccaaacattc tttattttta
     6841 tatactttta gtaacttttg gtccaatttt attccattgg aatatattac tttattattc
     6901 gatttttttc ttattgctac tttgactctt gcctttaata cagataaaga aaatggtttt
     6961 gtaatataat catcaagccc tatttctaat ccttttactt catcaatttc tgagtctctt
     7021 gcagtaagca ttataattgg tatatctgac ttttccctta ttatttgaca tacatcaaat
     7081 ccatctccat ctggaagatt gcagtctaag attataaaat ctatattttt actgttaaat
     7141 atatttaatc cttcttgttt tgtataagcc ttaaatacat taaatccatc attctcaaat
     7201 gcataaaaaa gaccttcatt taagtttata tcatcttcaa taattaataa gttcaatttt
     7261 actctccttt agatttttta gataaataat tattttcttg cattatcata ctaaaaatat
     7321 tttaatttta tttatttcac taaaaagatt gtatttaaat ttgttttcaa aataaaaaca
     7381 tcataatcaa gcatatagtt atatatttcc caatttaatt acgcttaaat tgaaaatata
     7441 actttattct tatataatta aattatacaa caataaagtt atatttttta aatatatttt
     7501 tactaggggg aatagaattg aaaagggttg atgagagaaa tactatgttt gcaagagcaa
     7561 attacaaaaa agatagtact gcttataatg actattataa gaaaaatcct gacaaaaaag
     7621 aaattgatga tagtataaga aatagaccaa atttatgtag tgaaggaaca atgacttaca
     7681 atgagttaaa ctctccaatg gcaagtagtg cattcgattt tttatctgat atcaaatctt
     7741 tatgcgaagg aaaagtttct gatactaaag tagatgtaga tgcaaagtca atgacaaaaa
     7801 aaattaaggg tcttgcaaag cagtatggag cttctgtagt tggtataact aaattaaaag
     7861 actatcatat ttatacaaat aggggtagac atgaagaaaa ttacggtgaa gaaattaatc
     7921 taactcataa gtatgcaatt gtatttggat gtgagatgga taaagaaatg ataaatagag
     7981 ctccaatgat atgtgaagtt atagaaacat caaagtgcta tgtggatgca tctatcgtcg
     8041 gcatgatttt aagttactat ataagaaatt taggatatga tgctagaaat catatggatg
     8101 ctaattattt agtaatgcct gtttttatag ctcgtgatgc gggtttagga gatataggaa
     8161 gaaatgctat acttacgaat aaagattatg gctctagact tagattaggt gttgttacta
     8221 cagatattcc tcttttagaa gatgaatatg ttgattttgg actagaagat ttttgtaagg
     8281 tctgtaaaaa atgctctttt aactgccctt ctcattcgct ttctaatgat attaaaattg
     8341 gtgatgatgg aaaatataat tgggtaattg aacatgagac atgctatatt aaatggagat
     8401 atttaggtac agattgtggt atgtgtatat ctgcttgccc atttagccaa aatctcgaaa
     8461 ctataaagaa tactacttca tttaagggta ataatgagct tattcaaaaa gctttagatg
     8521 agtatacttc aaaatttggt aaaagaatat ttatacctgg aaatccatct tggttgaaat
     8581 aattttattt aataaagaat ctgtactaga atttatcata ggacaggttc tttattaaat
     8641 atataaattt ttaaatactt tttctcgttt ataacaattc agcaatgttt ttagtctata
     8701 attgacatta taaaatatta aactaaaagg agtaactgta tggaaaaaat attagtagta
     8761 gaagatgatt ctatattaaa taaaactcta tcatataacc taattgaaga tggatatata
     8821 attacttcaa aatttacagc taaatctgca ctgaagtcta tttttgaatg tgaatttgat
     8881 ttaataattt tggatattaa tttaccagat aaaagtgggt ttgaattatg taatgaaata
     8941 aagggaaatt acaatatacc aataattttt ttaactgcta atgatatgga atgtgatatg
     9001 attaaaggct atgaacttgg agctttggat tatataacaa aaccatttaa tatcaatata
     9061 tttaaacaga aagttaaagc ttttttaaat catcttacaa taaaaacaaa acaagattac
     9121 tatagggatg gatatcttga aattaacttt tctgagttat ctgccaatat aaatggaaat
     9181 caaattatat ttacaccatt agaatatcgt actttaaaac tgcttactga aaatcctaaa
     9241 agtattttaa caaggaaagt acttttagaa aaactctggg atatagatgc taactttgta
     9301 gatgaacata ctttaacatc tgttataagt aggattagaa gcaaaattga aaaagacaat
     9361 cttaagtata tcaaaacggt atatggtatg ggatatatgt ggttaggtga aagaaatgaa
     9421 ttttaaaaaa ttttctttta gttctattta tttaataatt gctgttggta taatttttac
     9481 agctatttta tttagtatct caatttatct aataactggt gatttaaaac taattgctct
     9541 attattaata tatacaatgg tactttgtgg atttatagca ttaataatat ttttacttca
     9601 taaaaaaata gttatatttt gttctaaaat atgtcacact ttagatagta tgatgaatag
     9661 tgaagacaat attgaaattg aattagaaga agaaactatt ttatcacgta tcaatcacag
     9721 attaattaga atgtatgaag ttattcaaga aaatcgcaat agtattgcta tagagaaagc
     9781 tgatttacaa gaattagttt ctgatatatc tcatcaaatt aaaactccta ttgcaaattt
     9841 aaaaatgatt aatactactc tactaaatca aacatttaaa acagaaattc aaaaggattt
     9901 tttaatggat atggaaaacc aattagataa attagatttt ttaatgcaat ctatgataaa
     9961 aacctccaga ctagagacag gtataatcac actatctaaa aagaaaaatt caatttatga
    10021 aacaattgct acagcactta gtggaatttt gtttgatgct gaaaaaaaga atattgaagt
    10081 tacagttgat tgtgacccaa atttatatat aattcatgac aagaaatgga cttctgaagc
    10141 tatttttaat atattagata atgctgtaaa atacacaaat tctaatggta agatacgtgt
    10201 agctacagaa tgttgggtta tgtatacaaa aatagatatt attgataatg gaaaaggaat
    10261 atcagaaagt catcaagcag aaatctttaa aagattttat cgtgaagaag atgtccatga
    10321 catagaaggt attggaatag gtctctatct ttcaagaaaa attatatctc ttcaaggtgg
    10381 ctatattaaa gttacatcag atattggaaa agggtcgact ttttctattt tcttacctaa
    10441 taaataaatt tttaaatatt tcatttgtaa tgattctgca acattttggt tttatactgt
    10501 atttacaaga tgttaaggag gtattattat gagtatttta caagtaatta atttaaagaa
    10561 atattatgga caaagttcaa acattacaaa agctcttgat ggtgtaactc tatctattga
    10621 aaaaggcgaa tttatttcta ttgtaggtac atctggtagt ggcaaatcca cattattaaa
    10681 tatgatggga ggattagata atcctacatc tggaaaggtt attataaagg gtaaggaatt
    10741 atctactatg actgatgaac agcttacaat ctttagacgc agaaatatag gttttatatt
    10801 tcaaaattat aatcttgttc ctttacttaa tgtatatgaa aatattatct taccaatagg
    10861 attggatgga gagaccatag ataaggattt tataaatgaa attataaata tgctttctct
    10921 tgaagaaaaa ttatatgata taccaagtaa tctttctggt ggtcaacagc aacgtgttgc
    10981 tatagctaga gcacttgtaa caaaaccagc tattatacta gctgatgaac caacaggtaa
    11041 ccttgatagt agaactagtt cagatgtact gagtttactt aaagtgacaa gtaaaaaatt
    11101 caaccaaaca attgtaatga ttacacacaa taatgaaatt gcacaactaa ctgatagaat
    11161 tattcgtatt gaagatggaa aaattgttaa ctaggaggtg tagtatatgt ttagaaataa
    11221 taatcgcaca tttataagaa aaatagcttt aaatgattta aaaattaata aacttaaaac
    11281 atatctttca ggtatcatta taatgatttc tacatgttta ttattaacag tgaccttagt
    11341 ttcatataat gcttctgtag atttagttaa tgcatctcca tatcatgcta tatataaatc
    11401 tgtagatgaa aaagcaaaaa atatactata taaagataaa aactttagtg gcgtaggaac
    11461 ttacaaatta attggaagta ataaaaagac agattgtatt atgtcgattg tttatgcaga
    11521 tgatacagca attaaactta tgaattttca accattgaaa ggcaaactac ctacaaataa
    11581 aaatgaaatt gctatttcag aaaaatattt acaggaattt ggtttagata aagatattgg
    11641 agatagcatt aaattaaatt attatgatga tattacgaat aaagaagttc aatgtgattt
    11701 tattattgtt ggatttctag aaaattatta tcaagataat gccaagcaat actatacagt
    11761 cgtttcaaat gattatttta agaatatagc ttctacacct ttacaaaata aaagtaatac
    11821 ttttgacgag ttaagacctg atactgtaga tgtattagtt aaactatcag aagaaaatac
    11881 agagaaagat tcagcttcaa ttaagacaca gttaaataaa attggtttat ctttaggtat
    11941 taagaaatat aatatatact taaatgataa ctatattgaa tcaaatttaa ttgatggtga
    12001 gcagattatt acagttttag tagttggaat agttgttctt ttttctagcg tttttgttat
    12061 atatagtatt ttttatattt cagttgttaa ttcagttcag atgtatgcta agttgaaatc
    12121 gttaggtatg acttcttttc agttaaaaaa gattatttct ctacaaggaa atattttgtc
    12181 aattattttt attccattag gggttatagc ttcttgtata attgcttaca taattcaacc
    12241 attagcatgg caaatgatag ctgatttatt tataatttta attttatcac tggtaatgtt
    12301 tttaactgtt agaatttctt tatttaaacc agctagaatt atttctaaaa tatctgctat
    12361 tgaagccatg caatatacag aaactaaatt tagaaaaaaa gcaaaaaatt ttagttatat
    12421 aaatataaaa aatctagcat taaagaatat cgagtcaaat agaaagaaaa atttgattgc
    12481 attaatatcg ctaagcatta gtggagtttt atttatttca atcgctaatc ttgctaattc
    12541 aatagatttc aaaaaacaac tatctcaaca atttgtctat aacgaagatt atataattag
    12601 cattaagagt gataacttat atgagcatat tacagaaatc caacaaaata atccactaac
    12661 agattctctt aagaatgaaa ttaaatctat tcatggagta aaaaaattaa ttgaatccaa
    12721 atcaattaaa agtaatatta tagaacctta catcaaagat agagacgaaa aacaatttat
    12781 tactctaatt aaaggtatta cgcctgaact ttcaaaatca cttgaagaat atattgtcag
    12841 tggaaaaata gactatacac atttagattc agatagttta attataaata aatatagaac
    12901 aaaatattat ggattaaatt taaaagttgg agatacagtt acatttaata tctttagtgg
    12961 aaatgatatt attaaaaaga aaatgaaaat tataggaatc attgacaatt ccaatacagg
    13021 aggtatgttt tttgcttcag acaaaacttt agaatctcta acaccatata atacaaattt
    13081 agatttttct attttaatag ataaaaagca ttctaaagaa gttgaagaaa agttgttaag
    13141 tataattcag aaaaattcaa atttaatgct ttattcatat gaagatgatt atagaatgat
    13201 tactagagca tttcaatata taattatagc ttcatacatt tttgttggtt taatttcttg
    13261 ctttggtata ctaaatatga ccaataccct aataaacagc gttttaatta gaaaaaaaga
    13321 gtttgcctta cttcaagccg ttggaatgac aagaaagcaa ctgcgaaata tgctttatag
    13381 agaaggtcta aacataagta tcaaagctat atgtacttca agtatactag gttattttgg
    13441 ttcaaatcta ctttgcactt ttataaaaga tgtaattaga ttagacttta ttaattttaa
    13501 attttctata tttactattt taattttttc attcgtatta attggtatac aagttttagt
    13561 gactgaatta cttgtaagaa atatagaaaa aaaatcactt acagaacgct tacgttctca
    13621 gtaagaatgt atataaaaaa tagccatttt ttatacaagt tttaatatga ttttttaaaa
    13681 gctataggga tagaaaattt tgactttaca taattttcta tccctatact tgtttttatt
    13741 ttatacagat acacttactt atatctccga gcaacatcac cagagacata aacaagcata
    13801 ttccttattt tctaaatatg tcaattgcat gacttgtaac ccctactaaa tcttcacgtt
    13861 cacaagtagc aaggtgatat tttaaatata atgcaaatgc atcatcatcc atagagtcta
    13921 ctgcttcacg catatacaaa gccaaaccat ctgatgcgac ataatgcagt ctagttacat
    13981 taaatgcaga catcaaatca tcaatgtttt ctttacgtac aagctcaaac aaatcctttg
    14041 gttcagactt agcagcaaat gtctgtggat caatcaaacc tttttcaata tattcagata
    14101 cattaatatt tcctctatgg aacccctcgt caatgagaca gccatcagat ataacatatg
    14161 cagcaaaaac cacaccacca ggttttgtta cacgaattgc ctcatgtaaa gcttgttgtt
    14221 tatcttcttt gttataaaga tgatatagtg gtcctaacag taacgtaata tcatatttat
    14281 tatctgggaa aacagataaa tccatagcat taccctgagt aatgcttata ttttcatttg
    14341 attgagtatt tttatggaaa acctctatat tatgttcgac taattccact gcgtcaacat
    14401 catatccctg acgtgccagt gtgtgagaat aacgaccagt tgcagcgcca atttcaagca
    14461 cacaatcacc tgattttatg tacttatcaa tgtaatgcat agtagtgaga aattccacca
    14521 ttccatgttt taatgcaagt cgactatctt catcataagt attataaaag tcaattaagt
    14581 attgttttgt ttccataatt tcacctcttc tatattagtt aataaacatc aaatagacaa
    14641 attccaatct gatttttctt gtcttataaa agctgttaat atatttttca acattttttc
    14701 agcactatat ctatctaatg ttaatttttt tgttgctgtt aagacagcta tgccatgagt
    14761 ataaagaaat aaatctaaaa atattacttt tgcacattcc aaatctattc caatgctttc
    14821 cgaaaaaagt cttgcctttt tttcattgtc aatttcttta taaaaatcat tcgcctcttt
    14881 catcatcaaa tctaaatcat ttataaataa caacttaaat aaatacgttt cttcttgagc
    14941 aaattcaatg tatgaaagcg gaagcagtaa ataagggctt acacttttag aattacgata
    15001 attaacaaca tactgattat aatattcata tgcaaaagca agaaattcat tttttaattc
    15061 attcatgttt tcataacaag taaaaattgg tcgagtggaa cactgtaatt tacttgcaat
    15121 actcctcgca tttacagctt caaatcccac ttctcttgta atatccaaga ctgtattcaa
    15181 aatcatttct tttgttatct ttactttagg tggcatatat gttcacctct ccattaaatt
    15241 tttataaaac acatgtgtta tataactata gtaacatatc aagaaattat gtcaagaaaa
    15301 agctttgcat ttttttgcaa ttcctaaaac ttataaataa aacatgagtt gcaattaatc
    15361 tattaataga taacagcaac tcagattttc ctttaaatac tcttcttttt gtctaatatt
    15421 tcatatacac aatcttctat tgaattaata tctttttctt ttcttttttt agaatcaatt
    15481 aaaatgaaat acagtataaa acctacagca agtccccatg ttccgccttt agcagcaatt
    15541 acacctccca taagtccaat caatcccttc tcaacacctg tattacatag ctccattgat
    15601 agctttgtac ataaatatcc ttgaactaaa agtgttactg acaatgctaa tggtataaga
    15661 ggtgtagcaa tctgcgcaat tggataaaaa cagataaaaa ttgctgaaac ccatactaat
    15721 gtacccatgc cactaaaaac agactgcatg gcttcctttc caaccttgta cctttgagca
    15781 acacttcctg taagtgtcgc agctaatgga ccgcacattg gtatatatgg agaaaagatt
    15841 gccataaaaa tatttcttat cccacttata acattagacc tatttgaatt gaaatctata
    15901 tattcatctt ttctattttc actttcagaa agtactaagt tttcacctgt tataaaatca
    15961 ccaaaggcaa ttatgtatat cataaaagcc attggtatcc cttgtataaa taacattaca
    16021 cttggaaaac ctattccaaa aggagatact gtatttatca gatttttaaa atctactatg
    16081 taaactaaat tgtcaaaact aaatctagga atttctattt ctttactaaa tatacctact
    16141 attaaactca ctaaaatagc tgatactaca ccatattctc ctaaatgaaa taaaaattta
    16201 ttcttaactt ttagagtatc aaatctttca gaaaacatta caaaacaagc aaccaaaatt
    16261 cctactgtta tagatatagg ataagaatca aatctccctt taggattaaa ctctgatatt
    16321 atagtcgata taccgacacc cattagtatt ccacctttaa tagaatttgg tatcttacta
    16381 actaactttc cagctacacc agttataccc attaaaataa atattattcc taaatctatt
    16441 tgtaaagctg ttaatgcttg tattctttca atgcccatgc tgaactttaa caaatatgca
    16501 gttgtaagag ttatagaagc tgtaatccag ccaggtacaa ctgggtctcc cataaaaaca
    16561 tgcaaacagt ataatattga gttaataata gccatactcc atgccacttc atagggtaat
    16621 cccaaaattt gtgtactaag gccaattgtg cctaaacttg tcatacttga tattatacct
    16681 tgaatagctt ctggtttctc aaatctgtaa tgtataaatg gaattcttat cttaaacatt
    16741 ccaaatttta tatgaggttg ttcccttcta gcgagtcttt ccataacttc actccacctt
    16801 tcaataactc ttataagttt acaactttat aattgtttat aaattatcaa tgtcattaaa
    16861 attagtatat aacatgcaaa ttatataaac aataaattaa atctataaaa ttgatagatt
    16921 ttgtgcatat atagaaagac cgcttcattt ttagttactt aaatcactta atagtagtta
    16981 tttaaatcac ttgataaagg ttttttataa aattcaatag ttctattcca tgattcaaac
    17041 tgtccttttg aatttccttc tacagttcca cctgaagaaa aaatccctcc actcatactc
    17101 atacataaag tctcctttag aggcataata tatggaattg gtaacggatg acccatatta
    17161 ttgtatgtta ataattctac gttatatgga taattatttt cttcaaagat ttctaatgcc
    17221 tttaaacaag cttcataact attccatatg ttatcttctt ttccagcaat cataagtata
    17281 tctgctttgg aattttctat ttttattcta gcctcttttc tattctctgc tcttttaaga
    17341 cttttcttat aagtactatt aaatccaaat ggaatacctt ttgatatatt ttttttctgt
    17401 tcttcaaaga atatatcatt gtctactttg atatatggaa atggctctcc tttatatgac
    17461 catgatgaag tatcatttcc actcatcaat ccattaagag cttggaaaca ataaatgtga
    17521 ggttgtgata ctacaacctt tttaatttgt tcatatcttg aagcaagtaa aaaggccaat
    17581 tctccaccct ttgatgtccc atgtacaaat atttctttag tactggtaat ttcattttca
    17641 ctaatccact caaatacttt ttcaaaatat tctaatggaa cctcttctaa tttatctggt
    17701 aacccttcta aagcaaagta tgcaactgtt agtacattga atcctctaga tgcaagtgga
    17761 gctgctaaaa ggtctagagc gtctaaattt ccatcagaac cacctagcat taaaattgtt
    17821 ttattattag aattttgttt ataaaataat tttcctataa actcatcaga tatgtatttt
    17881 gtggtaatat cttctgattt aaatattctc tttaattcaa cttcttttct ttcaatagaa
    17941 gtttcaaata tcatattcat aattattggt ttatcaatag atatattttc tgcaaaattt
    18001 ttaccttcag ttttagattt ttccagcgaa taaattaacc ccatacaatt atcagcctta
    18061 taagtacctt ctataggttc aactaaatct aaatctactt ctcctttttc atttgaacaa
    18121 aacactccat aagatgaaaa ttcttctccc ttacaccatg gaaattccat tttcacactt
    18181 attttaagtt tagtatttgg aattaagtct gaaaccataa tcttaagtct ttcatccaca
    18241 aatgattcta cattgtatac atttactttc ataatctatt tctccttata ataattattt
    18301 atgttttata taaaacagct ttatctaata caagataaat aaatttttaa attaagacac
    18361 catattgact atttcatttt atcaacatat ttcatctttg tcaacatatt gtctttttta
    18421 gtaatttaaa attatggtct atatgaaatc cagttgattt ttggctaatt aaagatagga
    18481 atgctattag gtttagaata tatatattca aaataaaaaa ccactcattt aaagaatggt
    18541 ttttaatata aactatttgt tattaataag gatatttttt tatttaaatt tatataatcg
    18601 aacatctaat atttaatgtt taataaaatt atgcatttgc aaaagtatat atggcattaa
    18661 ctcttgctac atataaattc tcatcactac atccaatata tctagcatat ttttcaatct
    18721 tttcttttgc attttcaaca tcattttcat cgctctccat taactcattt atataaatat
    18781 ccattaatga cgctttatat tctcttatat catagcctaa aaattcagtc aatctctcaa
    18841 tttccttact aaaatctcca tcttctttta ccaaaaattc gtatacatct tccctatatg
    18901 gatttaaagt tattatttgt tttactactt tgcattcgtc agccttccca ataaaaccat
    18961 ctttataatt attaaataag gcattagatt tttttataga ttcttcatca atataagaat
    19021 gtaccgcagt tatacgattt tctataaggg catctacaac agcataatga attttaaaac
    19081 aatcattgta taaataattg ctcattttct ttattgtcga ttctatttct aagaagctag
    19141 cgtgaccatt ttttttattt ttatttggta taataaaagt tttattcaaa ttattaaaaa
    19201 gtctcttaaa atctaaatat ttttcacaat aaacactttt aaaggtatcg atatctatag
    19261 ttataattcc aaaacttact atagtctcaa ttcctttctt aaaagactct tctatatatt
    19321 tttctcctaa acttaaacct ctatcagata tttgctttag attttgaaaa ctatttaaac
    19381 aatctctttc aaagttttta gatatttcta aagcatataa tttaaagatt ttccttatct
    19441 tattataatt attttttgta tcatcaaatc tgataatagc tccatttaca tcaaaagtaa
    19501 tcattgagtt ttaacctccc cattagtttc atcaataaaa taaatttaat ataaacttta
    19561 taatcataat attaaactta tttatactat aattattaat ttgatgataa aagctatttt
    19621 ccttctaaat aaaaaatttg tctaaagttt ctaaaaaatt tagacaaatc ttatgttatt
    19681 actctttaaa ttatttttaa attattccaa atacgccaaa taatattggt ataaatatag
    19741 ctggtaacaa attggctact tttatattcg ttaatttaag tacatttaga ccaagtccta
    19801 taattaataa acttccagct gctgtcattt ctgtgactac aggatcactt aaaaatccat
    19861 ttaaaaatga tgctcccaca catataatac cttcatacaa aaatacagat attgcagaaa
    19921 acattacacc tatacctaaa gaagcagtaa atataattga agctattcca tctataacag
    19981 attttacaaa taaagtatca tgacttcctg taagaccact ttctaatgaa ccaactactg
    20041 ccatggctcc tatacagaat aataaactag atgatataaa accctctgct atagaatctt
    20101 ttttatttcc tttagaaaat ttactttgta aaaaagcacc caacttattt acccatttgt
    20161 caatatctat taattctcct atcagagcac ctacagctac agatattatt gtcactaatg
    20221 tgttttttcc ttctagtgct cctgatatac caatgtacaa tacacataaa gcaattccat
    20281 tcattatagt atcgcttact ctttgaggta atcctccttt tattataaga cctactatac
    20341 atccacctgc aattgctaga ctatttgcta ttactccaaa cattttttct cccctcgatt
    20401 agctaattga aatttatcct attattaaaa cctaaacatc tatactttgt attggatatt
    20461 ttaaatattc caaaaattct tcaaaacctt ttctagatat atttacagtt ttagtattta
    20521 tgttaggatg aaaatttaag taatcttctt taattacatc tttatctatg taaagctcaa
    20581 cttttctttc tgtatcattt attaatgaaa aaggatttac acttccagga agaagtttaa
    20641 gtacattgta tagtctttca ggtgaagcaa acgataatct agaacttcct atcttatctt
    20701 taagtgagtt taaatcaact tgtttttctc ctttaactac aataagataa tacttatttc
    20761 ctttggcatt tcttaaaaat aaatttttac attgacaccc ctttgttatt ccatctaaaa
    20821 tggctaattg ctctgctgta tatacagctt catgctctac tacatcatat tcaacattta
    20881 atttatccaa aatatcatat attttagttt cattcatgct agattcattt tcatcttgtt
    20941 cttttttatt atttaaatca ctcatacttt tctccttaaa cacttcatca taatatcatt
    21001 gtgaggtatt attctactat agctattgct cttactggag ctccatcagc atcattatac
    21061 ttaaatggtg ctgctataaa tagaaatttt tctggaacat tttctaaatt tgtaagattt
    21121 tctatgatta tttttccttt ttcaaataaa atatgatgca cttcaaatac tgatgtgtca
    21181 tatctatcca ctgatagcat atctattcct atgcctttta tattagtatt agctatatag
    21241 tttgcagctt cttttgttaa tgtaggataa cctacatagt actgcttttt atcccaaaat
    21301 tttgaccaac ctgatttaaa tattataaaa tcactatttt ttatttcatc ttcatatttt
    21361 tttagatatt ctaattctat attctcattt cccttttcaa gttccaatac ataagctttc
    21421 ccaacaaaat tttctatatc caacttatct agggtctcac ctttagtata catatgtctt
    21481 ggtgaatcca tatgagttcc attgtgagaa aatacactaa ttaatgtttc ttgataacca
    21541 ttttcttcaa taatagctac tttcttgata tctggtctat taggctctgc atatacaggc
    21601 atgtcattat gtgtcacatg agttaaatca aaaaccttca tctttatata ccccctatgt
    21661 ttttattttc tattatatac ctaaattcat tttatttcta tcagatttta ttccattata
    21721 taccatacta ctatatattt tttataaacc tcaagggatt taccgaaaaa tcttggaaag
    21781 aattatcagt aaatataaca tcttcaccca aaaaacgttt ataccttcct tcttctttct
    21841 ttaaagtaat tctctgtttt tgtgaatgat tattaaaaac cattatctta agaggaggtt
    21901 tttttctatt agtctgcact ctttttaaca tgtattttat gtgcatatca gcttcaggaa
    21961 atgaatatcc acaaaatatt aaaaggtcag tatctcttaa agttttttct gtttcattcc
    22021 aaacattact taaaaatatg tttgacatat tcttaaaata tgttggtggc actataatag
    22081 gttctgttaa ctcaccacac tctaaacatt ttgtctctga actattttca ataagcttca
    22141 tgacaccacc ttcatgtggt gtaagagtta cactattaca aatggggcaa taaagccaat
    22201 tcaatgaccc atgtatctta tataatttga ttaatggttc ttttggtttt ctccaattta
    22261 actcctcttt aaaatttgta aactccacac cataatcaag cataattggc aaatcattct
    22321 tatctcttaa tccagtaact gtattgtcta tatgtatatc ataatttgca ctaataaaag
    22381 tagtatcaag aagcaaatca ttttccaaaa gattttcaac taaaagttta tgatatttat
    22441 tatctgttct aggtgtatta tgaattgctt cagccattag cagtattaaa tattgtctta
    22501 aaaaacgtat actatcactt ctgttattta gattttctag tccaaaattt ctaaatgctt
    22561 cttttctttg ttctgctaag tctaaaagac ctaaaacttc ttcaaaggta ggaaaatttg
    22621 cattatctat attatctttt actatatcaa tattgaacat ctgtttaaag aaaatataca
    22681 attctctatt cattttggta ttataatttg tgggatttag ttttttaaaa tattcgctaa
    22741 aaagttcatt ttgaataggt agattctctg ctgctgaagc tcctgctcct aaaaatattg
    22801 ctattttcat atagttcctc ctaatataaa tattactcct atttttatat ccattaaata
    22861 tccatatcat ttattttatg atacaacaat aaagctgtct tttactagaa aaaatcctat
    22921 tacaagacag ccttatatta tattaaatct ttaataattc gtaaatagaa agagcatata
    22981 tttgagtagc ttttataata tcctctacta ttaagtattc attagcttgg tgttctgttt
    23041 ctggttttcc tggaaaaata gctccaaatg caacacaatt atctaaagct cttgcgtatg
    23101 ttgctcctcc agatgaaagt ggtgttccat caagaccagt ttcttcttca tatacttttc
    23161 taagtgtctt aacaagaaga gtatcttctg gaacatagat tgaatccaag aaatcatatt
    23221 cttcataatt taaattgtat ttatctgtca ttttctttaa ctcttttaca aaatcatcct
    23281 ttttgtatgt tactggaatc cttacatcta ttcctgcaaa ttctttttcg ttatcaatag
    23341 ttactctacc tatatttact gttagtttac ctgatacatc atccttgcaa ttaggaatta
    23401 tattgtttcc attagcatct tcaccaataa cttctgctaa aaacttaatt atattagaat
    23461 ctatacctat attattaaga gctatacaaa gtctagccac tgcatttata ccagtatcac
    23521 ttgctgctga atggacactc tttccaatta tacagatttt attaccttca acagtatatt
    23581 caaaatttaa tttatcaagt tcttttttta atttatcaga gtattttcca atatagattg
    23641 ctttacctgg aactgcattt aaagcctttc caactgacaa ctctatatca cttttgctat
    23701 cacaagtcaa atgaacttgt aaaagacctt tctctgcatt tgttattggg aatctagaat
    23761 ctggagtaaa tccatagttt ggtatttctt cattattttc tttatattta tttatacatc
    23821 tccaaagatt ttcttcatca gccccaaaaa tgaatcttat cctcttatta aagtctacat
    23881 ttaaatccat aagagctttc actgcataaa ttgctgatat tgtaggtcct ttatcatctt
    23941 gtgttcctct tccatacaat ttaccatctt ctaaaacagc ttcaaatgga gggtaattcc
    24001 agctttctaa atcaccttct ggaaccacat caacgtgacc aagtatacct atcatttttt
    24061 caccttgtcc tatttctgca tatccataat atccatcttt gtatactgtc ttaaatccta
    24121 aagatttaca taagtctaat atacctctta aagctttatc tacattttca ccaaatggac
    24181 aattttcagt tgcttcagat ataacactag gtatttttac actttcttgt attgaagaaa
    24241 tcatttcatc ttgtagagaa tttacttttt ctttaatttg ctctttcata ttatcaccct
    24301 tcaattttca tcataatata ctaataaata ttttgacctt ttaaaaaaaa aatatgctta
    24361 ttttttcagt ttttttataa attcaatcgc ttttaaagtc cccatttttg aatggtcaaa
    24421 tgtaagtcca ccttggaaat aaacattata aggaggtctt attggagcat ctgcacttaa
    24481 ttctatagaa gaaccttgta taaatgctcc tgctgccatt atgacttcac tttcgtatcc
    24541 aggcatgtcc caaggaacag gttttacata agaatcgact ggtgcagctt cttgtatacc
    24601 ttcacaaaaa cttattactt catcagcatt attaagtcta atacattgaa taatatcact
    24661 tcttaagtca tcatattttg gtaatacatc atatcctaat ttttcaaaag ctcttgagca
    24721 aaagatagct cccattacag cctgagatac tatataaggc gccaagaaaa aaccctgaag
    24781 tacatttcta gttgttccaa aagtaagacc acattctttt cctattccag gagaagtcat
    24841 tctataagat ataagctcaa tcaaatcttt tctaccagct atatatccac ctgtcaatgc
    24901 aagcccacca ccaggatttt ttattaaaga gccagccata acatctgctc caacatcagt
    24961 aggctctttg gtatctaaaa actcaccata acaattatca accattacta tagcttctgg
    25021 tttaacagac tttattactt ctatagcttc ttttatatca ctaattgaaa gtgattttct
    25081 ccaagaatat ccttttgacc tttgtatcat aacgagttta gttctatcat ttattttgtt
    25141 tttaattcct tctaaatcta agtttccatc ctctaaaaaa tctacatcat catatgtaac
    25201 accatactct ttcaatgaac ctttttcttc tcttatgcct ataactccct ctaaagtatc
    25261 atatggtcta ccagtaacag ataaaatttc atctcctggt ctaactatac cttgtataca
    25321 taaagtaagt gcatgtgtac catttacaat tattggtctt acaagagcat cttctgtatt
    25381 aaatacttta gaataaattt cctctatttt ttcacgacca atatcattat atccatatcc
    25441 tgtagtccag ttaaagtgca tatcacttag tttagattct tgcatagctt ttaaaacttt
    25501 atattgatta tattctctta tttcttttat ttcttcaaac ttatctttta tttcttccat
    25561 tatttcttgc gatagtttaa aagtatcatc atctattcca tagtagtctt ttaataattc
    25621 ttttgtctca ttaagcattt tatcacctgt ctctttatta ttttatttaa acatgtaaag
    25681 gagactattt tactaaaaca aataaattat agtttataag ttagtataag tctcctctca
    25741 atcatcttaa attaaattaa ttatctatct gttgttatta ttattgttgt tttggttttg
    25801 atttgttaga ttaatatact ttccaggttg tatagttgat acagcatgtt tataaatcat
    25861 gttttgttgt ctattatccc cttctattaa tattatataa ctatcaaacc cctttacaag
    25921 ccctttaact tgcactccat taactaaata tatagtaaca ggtatccttt ctttccttgc
    25981 attatttaaa aacaaatctt gtaaatttaa aactgtattt ttcattagat ttgtaccctc
    26041 ccaagattaa attgaacttc taaaatattc ctaccttata ttctatataa gttatgacaa
    26101 attttcaata ttaagttgta tacttaaaaa tttatatatt attttataga atctttaata
    26161 tataagatta tttcattttt taattcgtct atattttcat attggtctaa atcaaaccat
    26221 tttgctgttt tatatctttt aaaccatgtt atctgacgtt ttgcatacct tctagaatct
    26281 cttttaatta tttcaatagc ttcttcgtat gtgtattctc catccagata ctttaatatc
    26341 tctttataac ctataccttg cattgatatc atatcttttt taaatcccat acttaaaagt
    26401 ttttttacct cttcaaccaa tccattttta agcataatat caactctcat atttattctt
    26461 tgatacaaat gttctctatc tctatttaga actattatta ttggttgata ttcttcatta
    26521 aattttaaat cacttgaaaa atcattcatt ttcttaccac ttagacaaac ttcaagagct
    26581 cttatgaccc ttttagtatt gtttttatgg attctattag cagattcttc atcaagttct
    26641 tttaacttat tatgcatgta atctattccc ttttcagcaa gctgtttttg aagttcttct
    26701 cttaattcat tattggcatc agattgtgca aaatccatat tatatattaa agagtttaaa
    26761 taaagtcctg tgcctccagt tactaaaaca catttttcct tttcatttat ttcatgtata
    26821 tatttttttg ctctttgttg aaactctgaa actgaaaatg gatagtcagg cttaacttca
    26881 tctataaggt aatgcttcac tccttgcatc tcttcctctg taacttttgc acttccaata
    26941 tccatatatt catatatttg catagaatca gctgaaatta tctctgcatt aatatctttt
    27001 gctaatttta ttgacaaatc tgtttttcca acagcagttg gacctgtaag tattataagt
    27061 ggtatttttt tcataacatt cctttccata tattttaaat atccaaatat tttacataat
    27121 tcttttaaac attttttcta tttctgtctt agatatttcg accattatag gtcttccatg
    27181 aggacatgta aatggattct cacacttttc tagctgttcc aataaacttt ttatttcaat
    27241 atcgtatatt ttatcatttg cttttatagc agacctacat gccatagatg caaacctctc
    27301 tccttttaaa tcataattac ttgtaatttc ttctatatta tcaattattt gaagtataaa
    27361 tttctcggtt tcagggactc caaatatagt aggaacacat cttaccatta tatgattatt
    27421 gccaaaaatt tctagctcaa aaccaaattt cataaataac tccaagttat tttcaatttg
    27481 aagcatatct acatttgata cttcaataac cactgggtct aaaagtattt gcatatttat
    27541 atcttgtcta tagaacttct ccatatatct ttcatatagt attctttcat gtgcagcatg
    27601 ttggtccaac aaatacatag aatcgtcttt gctcaaaatt atataagtat taaatacaac
    27661 tccaattaca gaatatccgt ataaagaaaa cttgcgtttt gaactacaca agtattcttc
    27721 ttcactgtcc ttaatactat ctcctaaatg ataatttcct tcatttttaa ttccatcaac
    27781 ttgaaattct tcttgtatat tatcatttgc actatcttca cttagataat taatgttttt
    27841 tacatcatct tctacacttg catttaggac ttcacttact gattgaaatt catttattgg
    27901 tttttcactt gataaagtaa caacttcaat gacttcatcc ttacctttag taatattact
    27961 ctttttagga gtagattcta ataaatcatt atttcttaat ttataatctg tacttttttc
    28021 tctactgtta atagcgattc taggttgtgt ttttttatca ctataagtag catattttcc
    28081 aataagattt gagtgtatta atttaacctt taaaaaatct ctaagttcta tatacacttc
    28141 ttgttctttt tcaaatttaa tttcaagttt attggggtgt atatttacat ctatacatga
    28201 tgggtctact tctatattta agaagcacac tgcatgttta cctattggaa ttatagactt
    28261 ataactttct gttattgcat ctatgattat tttacttttt acaaatcttt tatttatata
    28321 gatatgttgt aagtttttat ttgaacggta tatattgtta ttacctatat atccattcat
    28381 tttaaagtga ttgcacttaa actctacatc tattatattt tccgttattt cttttccata
    28441 tatacttcta atagtgttta caagttttcc atcccctgga gtatttagca tctgtttatt
    28501 gttatttgta tatttaaatt gaatgtttgg attacctatt gcaagtttat ttatcaaatc
    28561 acttatattt atagtttctg catgagtaga ttttaaaaat ttctgtcttg ctggtgtatt
    28621 aaagaaaata tcttttataa tgatggtagt tccattagta gaacctattg gttctttcga
    28681 tattattttt ccaccttcaa catatatttt agttcctatt atttcatctt tagttttagt
    28741 cgtcatttct aattttgata cagctgaaat agatgccaat gcttctcctc taaaaccaag
    28801 agagtataaa tcatacaaat catcaatttt tttaatttta ctagtagcat gtctcaaaaa
    28861 tgatttttca acttcacttg aaggtattcc tattccatta tctgtaatct taattagact
    28921 ttttcctcca tcaattatat ctatggaaat tttatttgca cctgcatcaa tggagttttc
    28981 aataagttct tttactacag aagaaggtct ttctacaact tcacctgctg ctatcttatt
    29041 tatagtcaaa tcatctagga tgttaattat atttttcatt tttacacctc taagatttta
    29101 tttcttttgc ttttctttga agattataaa gagagtttat agcatctaat ggagtcatat
    29161 ttaaaatatc cagatttaaa atttcttctg atagtatatc tctatttaca ctatcaaaag
    29221 agatttgagt taaggctact tcttttacag aatcctcttg attaattttc tctttatcat
    29281 cattttgctt cattaaagca acttcatcat ttaatgcttt aaatttagaa tttaatttat
    29341 cataatcctt ttttatatgt ttgtaatctt ttactataag ttcatgttct atttttagag
    29401 attcatattt attcttaaaa ttactttgat ttaccttaac taattcttcg tccaaattac
    29461 tattaattat attattgttt tctttatctc catttattgc aacactgttg taaacatgat
    29521 ttttttccaa gtctttaagt atatattttg ctctttctat gacttcgtct ggtaatttag
    29581 ctaatttagc aacatatatt ccataactct tgtctgctcc ctgaggaatt atttttctta
    29641 aaaatattat tccttcccca tcttctttga ctgctattga ataattttta acctctttaa
    29701 actcttcttc taaatcagta agttcatgat aatgtgttgc aaataatgtc ttacatctaa
    29761 tatttttttg aatatattcc actattgacc atgctaaact tatcccatca taggtactag
    29821 ttccacgacc tatttcatct aatataacta aacttctctc tgttgcattt tttaatataa
    29881 gggatacttc gttcatctct accataaaag tagattgacc ttgagataaa tcgtcactag
    29941 ctccaactct tgtaaatatt ctatctaata ttggaatatc agcagactct gctggtacaa
    30001 atgagcctat atgtgccata agagctatta tagcagtttg tctcatatat gttgatttac
    30061 ctgacatatt aggaccagtt attatgttaa taatgttttc tccacgattt aaataagtat
    30121 catttggaac gaaattttcc tctcctacta tattttcgac aacaggatgt ctaccatttc
    30181 ttatatcaag tttattattt tcatttattg ctggttttac atagttattt atatgtgcaa
    30241 ctgttgcaag ggacacaaaa acatctatat tagctatagt cttagccact ttttgtattc
    30301 tatctatgtt tttatatatt gtatctctta tctctacaaa tatctcgtat tctaaagatt
    30361 taatcttttc ctctgcatgt agtatctttt cttctatttc ctttaattct ggagttatat
    30421 atctttctgc attactaagt gtttgctttc ttatatatgt ctcatctagc tttgcctgtt
    30481 taaagttagc ttttgttatt tctatataat atccaaaaac tttattaaag cctattttta
    30541 atgatttaac tcctgttttt tctctttccc tgttttctat ttcctttaca agaaaggctc
    30601 cattcttaga aatctctctt agttccttaa gttcatcact aaaatctgat ttaattatgt
    30661 ttccatcttt tatggttatc gtaggttctt ctagaatagc ttcatctatt aaattgtata
    30721 tatcatctaa tttatccatt tctgaaacat actcttttag gatttttgca tttgataaat
    30781 ttatagtatc ttttaaatta ggtaactttt ctatcgaatt ttttaagtga attaactctt
    30841 ttggtgttac tctctcaaat gctatctttc cacatattct ttctatatcg tatatatttt
    30901 taagtatatc atttaggtct tctcttaata taaagtcatc ttttatttct tctattacat
    30961 ctaatctatt ttcaatttta gatttattta tcaaaggttc ttctacatat tttcttaaaa
    31021 gtctacctcc cattgctgta gaagttttat caagtacgtg taataatgaa ccttttttct
    31081 tattccctct tatagtttga gtaagttcta aattagttct agtaaacata tctaaaacca
    31141 tatattctga tgaattgtat atatttatat tattaatgtt agatgttatt tgtttttgag
    31201 tattgtatat ataattcaga agaattgata agcttgactt tattagacct ttatcatcaa
    31261 actttagttt ttgtagataa acatctggaa aatattcttt taatatattt atatccaaat
    31321 agttatcact aaaactttca tttatgtata tattacttac agtagctata tctctaagct
    31381 tttctataaa gtctaaatca tttataatta tttcagttgg atggattttt gcaatttctt
    31441 caattacctt atcttcattt aaacatgttg cattagtttc tcctgtactt atatcaacat
    31501 aagttagacc tatattagta ccatccttat acaaagataa taaataatta ttctttttat
    31561 tttcaagtaa atttccatct aaaactgtac caggagtgat gactcttata acctctcttc
    31621 taacaattcc ttttgctgta gatgggtctt ccatttgttc acctattgct actttatatc
    31681 cattttcaac taattttgat atatatgaat tggctgagtg gaaaggaaca ccacacatag
    31741 gagcacgttc ttctaaacca caagcttttc ctgtcaatgc tatttcaaga gcttttgaag
    31801 ctactaatgc atcttcaaaa aacatttcat aaaaatctcc aagtctaaaa aacagtatac
    31861 agtctttata tctatttttt acttcaagat actgtttcat cataggagtt aatttattca
    31921 tatctatttg cattatcaaa cctccttctt ttctaattaa atattatatc atagtagatg
    31981 attattatta acacaatata taaaattttg acatataact aaaaacaatc caaaatgtat
    32041 actcacattt tggattgcta agtttaatgc ttattaaata aaaatatttt ttttacaata
    32101 aagatattat ttctttttta agtttaatga tattttcaag gttatcttca tttaactttc
    32161 tactatctat aacaaattct ttctttacta ttgatggttt ttttgaaata acataaattc
    32221 tgtttgatag cattatagct tcgtcaatat catgagttat aagaagtatt gttgaattta
    32281 cttttttctt aacatctaaa agccattttt gcatggatgt tttagttaat gaatcaagtg
    32341 ccccaaaagg ctcatctaaa agcattatgt cattagaatt tataaaagtt ctcaagaagt
    32401 tagccctctg tctcattcct ccagaaagtt ctgaaggata tttatcttca tatccgctta
    32461 atccaaacac ctcaaaataa ggttttacca ttgacctagc tttactcttt ttttcttttt
    32521 ttaaaattag tggtagtgac acattatcta ttatagtttt atatggcaag agtaaatctt
    32581 tttgatacat ataactatac ttaccattta tattcacttt gccactatca taatctgtaa
    32641 gtttagttat aatattaaaa atagtacttt ttccacatcc acttggccct aatatagaaa
    32701 taaactcacc ttcataaaca tcaatactta tatcttttaa aacttcaata tctttataag
    32761 tttttgatac attttctata gatactttta cttttttatt gtggtaaaaa ttcatttgtg
    32821 taagcatcac ttgccttcat atccttatct attaatttat attcttttaa gaatgatgta
    32881 tagttgttcc atacactatc tttcatttct ccccatcgtg gtgcatcatc tttgtacttg
    32941 cttgctaaat attcttgact ctttaaagct agtttttcat ctacttcagg tgcatgtttt
    33001 actaaaattt ttgctgattc ttctggattt tttattgcat attcataacc ttttgttgta
    33061 gcttttaaga attttttagc aagctctgga ttatctttaa gcacagtttc attagaaatt
    33121 ataagtggtg tataataatc caatctctca tctaaatctt ttactggtat aaagtttaag
    33181 tcatatccaa ttaaatcagc tttaactgca tcccaacctt caaatatcca tgcaaaatct
    33241 acagttttca tagctgcaaa gaaatcatct tgaccagtat taactatctt taacttatta
    33301 aaatcagctc catttttctt cataactgct ttaaaaacag cttcttctga tggagagccc
    33361 caaccaccat aagtttttcc ttcaaaatct ttagctgttg ttatattctt ttcttttggt
    33421 gatgcaaacc cagaagtatt atgttgtata acagttgcta cagcttttat tggaagtgga
    33481 tcttcactag tctttgcata agtaacttgt tcttgatagc ttataccaaa gtcagctttt
    33541 cctgttgcta ctaaagtttc tgctccactt tcaggtggtt gtactatttc aacatccaaa
    33601 ccttcttctt tatagtaacc cttatcaagt gctacaaata atccagtatg atttgtattt
    33661 ggtgtccaat caagcaccat agttactttt ttagaagaag tatctttatc attgtttttg
    33721 tctttattgt tatttgaaga acatccagtt attgttgcca atgtaaaaac acttactaaa
    33781 cctaatattc ctaacttttt aaaattcata attgcctccc tcaaatacct ttaatctatt
    33841 aatctatgtc ttataacaac tttttttaca gcctgtacaa ttcctacaaa cattaaacta
    33901 aatataacta ctaatatagt agatgcaaat actttatcaa gtgcatatgc actttttgac
    33961 ctaagcatat aaacaccaag tcctttcgtt ccacctagcc attctgcaac agttgctgcc
    34021 attacagcat acgttgcaga tattttaagt ccagaaaaaa atttatccat tgccatagga
    34081 aatttaaggt gaatgaatgt attaacttta tttgagtcca tagtcttaaa taaatttaaa
    34141 taatctttat caatattctc aatgccatct acaaaactta ttaatatagg aaaaaaacaa
    34201 gtaagcatta ccatcaatac ttttggcatt gttccaaatc caaaccatat cataaagagt
    34261 ggtgctataa ttattgtagg tatcatctgt gtaaccaaca taataggata aagacatttt
    34321 tttattattg atacaaaatc catcaatatt ccaattacca atgatattat aattgcgact
    34381 ataaaaccaa gtattgcttc tcctatagta accacagcat gcatggataa attttgataa
    34441 tctttaaaaa acacattaat tatatctact ggtgtgggta gtatatactg aggaacttct
    34501 aaaactccta ccatagattg ccaaagaata agtataaaca aaaatgttgc tattggatat
    34561 atactatctt tgactttatc tatgatttct aagtttttca tctatagtca gacctccagc
    34621 tttttcatct actctagcta ctgttatgac tctttcacat ccaagattaa aagggatttt
    34681 atgtaccttt tttaataatt caaaaacttc atcaaaatca ccttcaacag tagtgctcat
    34741 tgctcctatt tcacatttaa gaccactttt ttcaatttca gcaatacaag catctactac
    34801 tttatacatt tcttcttcac ctttgtaagg tcttagtggc attactgcta tgtctgcgat
    34861 taccatttaa atcactcctt taaaaataat taatacctac taggtatatt ttgtttttta
    34921 ttaaaaaatt ttattactaa aaagatatta tatttaaata aaagaaaagt cttatccaaa
    34981 ataggataag acttattttt atcttaactg gtctccctac actggcatta tccaaacagg
    35041 ttcaaagggt cgagataaaa tctcctctca gcacattagc tccccgaaat atattatatc
    35101 ttttctactt gattatacta cacttatcat attttaacaa attttttatt tatattagat
    35161 attttatccg ttcaaaaata tctaatataa attgtacatt attacaccaa ataaattatc
    35221 ttaaaatggt aatttatcaa catcttccaa aactagatta actgaatttt ctagttttta
    35281 ttttgctatt tcaagcatat ttggatgtat atcaattcca tacaaatatt gtgctaaatt
    35341 tctgttttca ctaatctaat ttgcaatcgt tacagattac agattagaaa tagcttttag
    35401 actctactta tctttatcta caaaaaatcc ttcaataaat tcagtaataa tattcagtaa
    35461 tctaaaaata aacctctatc tagattttta agatacaagc cttttaataa gtaccttttg
    35521 tgataatata aaaattactc gtcattatca tcaaaatcct cagcaaccaa cattttagat
    35581 aagaatcaaa ctctataata taattatttt atccaattta tttttgagaa ataaaatatt
    35641 tttttaattt ttaaattttt taatatgcac cttgaaaaaa gaaacgtttt cctatattat
    35701 ggtaatagta gaataaaact aaataggcaa aactagagaa atttagtgac gcaaagctat
    35761 agggactaag acttatgaaa tgagttatgt cagccagttg ccaaagagat attttctttt
    35821 tgtttttatg aatgtttttt agggggatat atgatatatg tttatggata aaatggatag
    35881 atgtacacat atactaactg catatatatg cagttcacgc gattactgca attttataga
    35941 tacacaatta aatgatttta tactagaata tggagagaat gtggtagaat cttgtttgca
    36001 tcaagtgatg gtattggtaa gcagatataa ttaaagaaat tttattttct agaaaaccct
    36061 ttttagtttt atctacgttt tataattcat taaaaaacta attaaaataa gcttcattat
    36121 atatttttca tgaagtaaac agttgagttt atctcatata ctgtttaccc caattacata
    36181 attctaataa tatagattcc aatgatttac cacgtttagt agacaaatat tcaacttttg
    36241 gaggaatagt cggataagaa atcctcttaa taagtccatc tatttcaaga tactttaatt
    36301 cttgtgacag tatcttatga cttatatgaa caacttcttt ttttaattga ctgtaacgta
    36361 ttggctcttg ttttaaaata tgccataaaa taattgccct ccatttactt cctatcacag
    36421 tcatggtaaa accaattgaa caattaaaat ctttttgtct ttcttcaata tttggaaatt
    36481 cttcaaaatt attatctata tccatctcaa tcctctcttt caaaatagta agtatctaac
    36541 taaaaagtgc attcttgttt agcgtactaa ttcgtaatat aatcttgatt gtagtataga
    36601 gccttacata atactacttt tttagatgta tatgtttaaa ttttaatata gacatatgtt
    36661 attttattaa ttttatttaa acagataata ataaataata atttatctca agttttctat
    36721 gtagataaac taatctaatg tttagaaagg attatataat atgaataaaa aatttcgtct
    36781 ttatcaagtt gactcattac aaaagaaaaa tttacaggta atccagctgg tgtaattaca
    36841 aatgctgatg gcttatctga atcacaaatg caaaaaataa caagagaatt aaataattca
    36901 gaaactgctt ttattttcaa taacttggct aaaaatcatc aagaaaatta tgatgtacat
    36961 atcagatttt ttacactaac taatgagatt cctatatgtg gtcatgctac tattgctgcc
    37021 cattatgtta gagcagtaga aaaccaattc aataacaaaa cagtatttca aaaaactggt
    37081 gctggaatat taccagttga tattataaaa aaagataatg attatgaaat aattatgaca
    37141 caaggaaaaa ttgaagtatc tccaccatta caacaaacct atcaaaaaag attacttaat
    37201 gcatttggta ttttaaagga agatattcgt acagattgtc ctattgccat atcatctaca
    37261 ggacattcaa aagttatgat tggaataaag gaattatcaa cattaaatag tttatctcca
    37321 aatttatatg aattatcgaa aataagtcaa gaaataaaat gtaatggaca ttatattttt
    37381 acattacata ataaagaaac tctacaagtt catggaagaa tgtttgctcc tgcaattggt
    37441 atttcagaag accctgttac aggaaatgct aatggaccct taggtgcgta ccttgtacat
    37501 tataaagtaa tacctacacc caacacagag tttatatttg aagcagttca aggagaagca
    37561 ataaacgcaa aggcaagatg ttagtaaaag taattatttt agataataag ccagttgaag
    37621 taaaaataat tggaaaagct gtaattgcat tctcaacaaa catagaaatc taattataga
    37681 ttccttctaa gtaaattttt gaacttttta atactaattt aataacaaac taatttatat
    37741 acaatcaaat taa
//
LOCUS       DAEORZ010000025        37617 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEORZ010000025 DAEORZ010000000
VERSION     DAEORZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37617
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.025"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(143..325)
                     /locus_tag="KRH39_001105"
     CDS             complement(143..325)
                     /locus_tag="KRH39_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBG8511024.1"
                     /translation="MKKEKRDLKFNFHKGGSGSFTPRMSVPKKWADEMGITRENPNIV
                     ATFEGDRIIIEKAKNE"
     gene            complement(1143..1295)
                     /locus_tag="KRH39_001106"
     CDS             complement(1143..1295)
                     /locus_tag="KRH39_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511025.1"
                     /translation="MDNFLLNILAGVIASLIFYIISKLFRQVKNHSAQKSGWEFDLKI
                     KFKKSR"
     gene            1814..4042
                     /locus_tag="KRH39_001107"
     CDS             1814..4042
                     /locus_tag="KRH39_001107"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG8511026.1"
                     /translation="MKNVNKNNIDKLFKNDCDLDRAVGEIENYAKFERQETHKIKKYI
                     DEKKSGVYNAIARAGAGETVLLLAETGTGKSYSFINALKKMNTKALFILPNSANVEQA
                     MCEYDIPGAYDLMPAPKALKEGNLSVFTWDKTEQLLNIDLSDYIIVVDEIHQTYTDSY
                     REKAIKNLNNIVAKCKGRIDITATPTKLEFEIYDYIIEYIKEKNTKYDVKLYDNINLD
                     KMIEIINKSKNSAVLYNNKKDLKYINELTTKKGDIVYAKIKAKSELYKRIIQHSDMGD
                     YELLLNTTTILAGVNIKNKDITDIIIAGFKDPATIKQYVARFRELKRVRVHIFNNFND
                     TEASNKVYEVNNIININFKRANKLKALYNELNSKRATGFETVGIDLVPFSIDSNVYYS
                     TKNKCYEIDKLFIISNTYTRYYNSRTIASLKVLLEEYFKDVKIENIEEQDEETKQGIK
                     EYKKVLKEIKTATLKTLEENKKYLVGYEQIKRGSKDFNLMSYMNYNKMSENDYKQAYI
                     KYNIDYLVDKADCYSEIELFSKYVLEHNYSLELSWKLANMGNNKRGNIFKKINNLIYR
                     ELKEEYPNLINDNLIEVVAYNYIDKLFGIGTAYTEYNLQELSNDLAIILGDNWRFATK
                     KLGNVLKNIFIVKAKQHRNFEALENYSYYKNNKPNTDFIDSDRIRIKKIERYITIEDI
                     EKDLKLEKGDLTLRNAIKIRKKKLLDNLDEIEKAFLNKELDKELRIEREIELVEQLEI
                     CI"
     gene            4289..4870
                     /locus_tag="KRH39_001108"
     CDS             4289..4870
                     /locus_tag="KRH39_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511027.1"
                     /translation="MKKILLCVIIGMLGLTLVACSSTASGDKIDGIEIVAKETSAEQI
                     KGDCNQDGSYNENGEYFQMGLDTAKASDYEYKIVNVSVKNASDEAIKLFQTGWNATAK
                     DGYEFEHIRVTDKLENQQVPANYSFDAQVKILVEKKMNVKEIILKYNLKDYSNLTNAM
                     KDASSGATKEDIQKKYPELYDDNWIEFGTIKFE"
     gene            5127..5270
                     /locus_tag="KRH39_001109"
     CDS             5127..5270
                     /locus_tag="KRH39_001109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511028.1"
                     /translation="MKIEYINTEEYTEFIEFIEFIVSAKKTKMKEFIYIKKVLFELEK
                     EDR"
     gene            complement(5288..5656)
                     /locus_tag="KRH39_001110"
     CDS             complement(5288..5656)
                     /locus_tag="KRH39_001110"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8511029.1"
                     /translation="MKERNSNEGKIPGICERVAEIRSFENISQKAFGEKINLSQSQIA
                     SYEKGYRIPTDRAIDDICRVYKVNKDWLVNGIGKKFIDPLIDLDLDPEIEEMAKLYLK
                     LTPSKREHIKKLMQKSLDSE"
     gene            5829..5981
                     /locus_tag="KRH39_001111"
     CDS             5829..5981
                     /locus_tag="KRH39_001111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511030.1"
                     /translation="MEYNIPYDQKDFLEFLKLGETIMGQNPLKFQRLIGRMQGIVDCL
                     EDEEEQ"
     gene            6063..6488
                     /locus_tag="KRH39_001112"
     CDS             6063..6488
                     /locus_tag="KRH39_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511031.1"
                     /translation="MIKVILCSTIDEYMEYEDVIGAKDSYLMDVIIGGECSEIDAIET
                     LEILKNSKARIVSEAEKTCLKFGIGNQISKAIEQGIKEAPKLKKGIHCDNCGASVGSS
                     EELTSMPVLENYNNNQSVSDQLGSIDVCPSCLLEITDLL"
     gene            6567..6971
                     /locus_tag="KRH39_001113"
     CDS             6567..6971
                     /locus_tag="KRH39_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBG8511032.1"
                     /translation="MINLETLCNGETKEKIENGFMEIFKNIQDPNTPATSTRSLTVKV
                     TLKPGKNRSHVNTQIQVIPKLASVLPSETDIIVEKDFRTGEVNANEYGNQLPGQIKLG
                     DLESKETSVTEETEVKESEEDNIRKFKSLKDL"
     gene            6994..7707
                     /locus_tag="KRH39_001114"
     CDS             6994..7707
                     /locus_tag="KRH39_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511033.1"
                     /translation="MLTDLFNRVMEVGARKIETREINGVTYTTDRLIPVYPKTVEEIE
                     LNTLDGLIEYIKSEVDKDAKGNRLIVQIKSPKSVRLCSELLEGGKRNTYVDCKALLPH
                     INFDCSLDSEEFNIMLQSSFVDTEDKNLLLKVAGNVKETSVKTVQDDGVSQVVAMSTG
                     VASVEDVILPNRVSLKPYRTFTEVDQPESEFIFRVKEGITFKLIEADGGAWRLEAIKN
                     IKEYLEKKLEGIENIDIIA"
     gene            7786..7929
                     /locus_tag="KRH39_001115"
     CDS             7786..7929
                     /locus_tag="KRH39_001115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511034.1"
                     /translation="MGAIERGEYVGNVALELREENPHLSKIDLIELALKKIREESEQE
                     AGQ"
     gene            7926..8237
                     /locus_tag="KRH39_001116"
     CDS             7926..8237
                     /locus_tag="KRH39_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511035.1"
                     /translation="MKIGKYLIVSQEQVDRNIECLREENKVRDDKIKFYERIMSNNEE
                     LIQILEEQLKEYKSELDVYSLFIDCILNKETRKAIAIYNRTKSIRIKQKCLNSIPIKK
                     G"
     gene            8284..8532
                     /locus_tag="KRH39_001117"
     CDS             8284..8532
                     /locus_tag="KRH39_001117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511036.1"
                     /translation="MKRYKFYIDEVISLKHKVIIEIPNEMSAIDISESIESECNLISD
                     LAGCIEKFGCKQIDFAEDVHGKRDILVNTCKKIEDSKN"
     gene            8556..8705
                     /locus_tag="KRH39_001118"
     CDS             8556..8705
                     /locus_tag="KRH39_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511037.1"
                     /translation="MSEFVQCCCCERTINIEENNYVQYEKEALGLVFTLYFCLNCVDE
                     LSEME"
     gene            8736..8909
                     /locus_tag="KRH39_001119"
     CDS             8736..8909
                     /locus_tag="KRH39_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HBG8511038.1"
                     /translation="MIMKNVLKLLAMYCFCPECGSDELGEGEGSLIVDEYTFHRKCKC
                     GFDVIVDEREDKI"
     gene            9021..9425
                     /locus_tag="KRH39_001120"
     CDS             9021..9425
                     /locus_tag="KRH39_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG8511039.1"
                     /translation="MNNTTLIGRLTRDPELKYIPGSGTAVSTFTIAVDRDYIKKDGTK
                     ETDFIPIEVMGKLAEVCANNLDKGRLVAVEGSIRVEHYEKDGEKRTYTKVHANKIKFL
                     DYKKDDTGKAYMFEPGGLDPQGFQAIDDGIPF"
     gene            9444..9833
                     /locus_tag="KRH39_001121"
     CDS             9444..9833
                     /locus_tag="KRH39_001121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511040.1"
                     /translation="MNEKWKQYIENVLESRGYCINRMPYVYSYDFLVNSNIKINVEIS
                     NLYKNEENEYHYFELEKEYHSCDIYILIALDEVGSALKILVIPASNLMGQKQIIINNK
                     SKYDKFDSRFELIKVYDNFYNDLDCVM"
     gene            9845..10207
                     /locus_tag="KRH39_001122"
     CDS             9845..10207
                     /locus_tag="KRH39_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511041.1"
                     /translation="MFKKVEKKFSGSVNPNFMEDDLTFKNYLALYCCIKPVKIHGNKK
                     VLASSTKACSALKIREHQKKARKKVKLINAKTGEIKEMAIDEAENFLNVKNLYAVIWR
                     GRPTRTGWYVKDVGNEGD"
     gene            10194..10364
                     /locus_tag="KRH39_001123"
     CDS             10194..10364
                     /locus_tag="KRH39_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511042.1"
                     /translation="MKEIKYNVIYFNSETLKMDKRSFDALKDARAFKKEKEKKYKNVE
                     IIKKTIIEKLIM"
     gene            10376..10948
                     /locus_tag="KRH39_001124"
     CDS             10376..10948
                     /locus_tag="KRH39_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8511043.1"
                     /translation="MAKKKRICSWCGKSFFTESKNRFCCKSCERKYKKDKEKNASSCL
                     VEETKEYLIELGNKKRRVDILRKEIDFLKDKEFYREINFNELGFKIHSSPKGIDEMIM
                     NNEDKISFKESEIDFIEYRLRLMDIYISELTEEKQQIIKFMYFYDGASKLSNEEIASK
                     IKCSRSNVHYKHKEALKELAEMLFESGSLL"
     gene            11127..11735
                     /locus_tag="KRH39_001125"
     CDS             11127..11735
                     /locus_tag="KRH39_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG8511044.1"
                     /translation="MGSKKPANPVKDKRMVLNIQEYLKEKNIRDYVLFVLGIGTGYRA
                     GDLVKLQVRDVRNAIDEGYFLIMESKKEKTKNIRKKNKKPRKAPIVPNLERVLKSYIR
                     DKKDYEYMFPSRQKSVTPYIGVERVTVILKEAGRYFGLKHITAHSMRKTYAYTIYEES
                     GFDIIRVKEMLGHSSIEETKAYLGLNEEQYQEYSMFLNDLIG"
     gene            12200..12769
                     /locus_tag="KRH39_001126"
     CDS             12200..12769
                     /locus_tag="KRH39_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG8511045.1"
                     /translation="MIFGYCRVSSKVQIDNNSLEQQEQEIKRHYSDAKIFREQHTGST
                     THRPIFEEMVNQMRDGDKLVVTKLDRLARNATEGINLIQNLFDSGISVHVLNVGLLEN
                     TTLGKFFITTLLAVAEMERNLILERTFAGKEIAKQNPNFREGRPKKYTKMQMEHALEL
                     LKTNSYTQVEKMTGISKSTLQRAKRKQED"
     gene            12798..13505
                     /locus_tag="KRH39_001127"
     CDS             12798..13505
                     /locus_tag="KRH39_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511046.1"
                     /translation="MICISIKNSNREKAFEIYKEKNGRIKLIDISKMLEEKSCNISRW
                     KKIDRWDYRLGINKKVGAPAGNQNALGHEGGAPVNNQNARTHGFFSKLLPARTYEIVK
                     YIEKDGGNSLDILWNSIVVQYAKILDSFKITHVKNKKDHTTDIKKNGNKIKEYEIQHS
                     WDKVTNAMKAEATAFKALSKMIKDYEELLHKNWDLATEEQKSRIENIKARTNKLTGND
                     LEIEDIEEIEAEIYGSN"
     gene            13492..14820
                     /locus_tag="KRH39_001128"
     CDS             13492..14820
                     /locus_tag="KRH39_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG8511047.1"
                     /translation="MAVTKKKTIPFLFGDKHKEYIKKCAINTYNIAEGAVRAGKTVDN
                     VFAFAHEIKVSKDKIHLATGSTSANAKMNIGDANGFGLEYIFRGQCHWGKFKGNECLY
                     IKGISTKHKQKIVIFAGGAKADSYKKIRGNSYGMWIATEINLHHDNTIKEAFNRIIAS
                     TNRKIFWDLNPDNPNSFIYTEYIDKYEEKDKTGDLVGGYNYQHFTIHDNITVTEERKR
                     EILTQYDVGSIWYKRDILGQRCVAEGLIYRLFTNNREEFKIQEKINDLMEINIGVDFG
                     GTGSGHSFVATGITRGYRDVIALSSERHFGEIDPDKLGLLFVEFCLKIVNVYGSITCV
                     YCDSAEQVLIRGLRSTAKKNGLGWLKIVNAYKSAINDRINLVSRLMAQGRFKYADDCK
                     TLEVALCSAVWDSKEITKNVRLDDGSSDIDTLDAFEYSIEKNMNKFIKYE"
     gene            14835..16211
                     /locus_tag="KRH39_001129"
     CDS             14835..16211
                     /locus_tag="KRH39_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511048.1"
                     /translation="MFSKILTWIREVLKKMTSEAGSNLVSDILISSEMIKNIELWSDM
                     YQDSAYWVNNVNVFSMNLASSISSEIARLATIEMDSKITGSVRADFLNEQFKKVLKNI
                     RIQLEYACAKGSIIFKPYVLNGNIEIDYIHSDSFFPTSFDASGKITGVVFVDQKIKNN
                     KIFTRLESHNLLKEGCLISNKAYVSSNNNSLGREISLQDVEEWADIQEQITIKSDRLL
                     VGYFKVPIANTVDTKSPLGVSVYSRATNLIRHADEQYSRLLWEFEGSELAINASEDLF
                     TKDINGNAILPKGKERLYRKLEIDSLRTTETFFEPFSPSIRDVSLINGLNEQLRRIEF
                     SCSLAYGTLSDPNNVDKTAEEIKASKQRSYAFVSDVQKELRNALEDLVYAMDALTTTY
                     NLAPVGKYEQSFDFDDSLIVDNKAEQSIKLQEVASGILKPERYLMWRYGVTEEQAREM
                     MPDDLMGE"
     gene            16212..17435
                     /locus_tag="KRH39_001130"
     CDS             16212..17435
                     /locus_tag="KRH39_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor capsid protein"
                     /protein_id="HBG8511049.1"
                     /translation="MLKSERLQELSNYFIYLYEQLEDFIIGDFSRRVSKAGTVTDMAE
                     WQIIRAELFGMSEKALKKKISSVLNITLKEVDKLFEEIALESISADSALYEYAKLTPL
                     HLSQSEELRDYINAVKEQTKSEFKNITGSLGFCTTINGIRRNKKLTDAYRQALDLAQL
                     QISSGAVDYKTAIKIAIKTISKEGVKVVKYDTDWVNRLDVAVRRATLTGVSQVSQKIN
                     NKVIDDLGTDIVEVTAHAGARSVGTGIKNHKAWQGKWYSLSGKSKEYPSLKRVTGLGH
                     GDGLGGWNCTHQYFAVIPGASTPTYTREQLENIDPPSFGYKGRTYTHYQALQQQRKIE
                     TAIRQTKREIIAYKNAGLEEDFTSASIKLNRQKQEYKNFSRVAGLLKRNERHQVQDFD
                     KSISQKSVWSNKKNK"
     gene            17498..18064
                     /locus_tag="KRH39_001131"
     CDS             17498..18064
                     /locus_tag="KRH39_001131"
                     /inference="COORDINATES: protein motif:HMM:NF018515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG8511050.1"
                     /translation="MNKEFLSGLGLKDDIITQVLESHSSTLEKEKEKYQSLEIKVGGL
                     EQQLKDANKEIKGYKDMNIEDIQKKALEWEAKYNDDTKALKEQLENKDYENTLKEFLG
                     KYKFSSELSKMAVFADLKEKKLKRENGTLLGAEEYLNQLKEKDPGAFLSDDVPGTVIS
                     STKGLQDGKVLNKNEDANIALRSLLGRE"
     gene            18069..19259
                     /locus_tag="KRH39_001132"
     CDS             18069..19259
                     /locus_tag="KRH39_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBG8511051.1"
                     /translation="MTTKISRENAEAIIREQIVNNILQDTPKQSIFMNLAKKMPNMSS
                     NTTRMRVLDVLPTAYWVNGDIGYKQTTRQAWDNVYMHASELAVIVPIPEAVVDDAEFD
                     IIGEVTPRVVEAIGQRIDSAVIFGENRPSEWQNDIITLARQAGNNVSVGSNPNYYDLI
                     MSEGGVLNKVEECGYIATGVIASRGMKAKLRGLKSTDGTPIFSTSMQGTTNYALDGAP
                     MYFPDNGSFDSSVAQMIVGDFNQAVYSIRQDITVKILTEGVIQDPNTKEIVYNLAQQD
                     MIALRVVFRMGWALPNPATRLDEDRVGCSFAYLEPQTPVTTQTVTITVKDNAGTPAVV
                     ADAIVDVNGSRMKTNSTGKVVYNLRPGNYPMKIKKTGYSTLNDNIVVSNSAVTKDITL
                     MINK"
     gene            19271..19603
                     /locus_tag="KRH39_001133"
     CDS             19271..19603
                     /locus_tag="KRH39_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511052.1"
                     /translation="MKTYIDYEFYKSLGGKIPQGSFDRYAIRATKYVDYNTFNRIEEV
                     TEEIKLATCEVADLIYKSNLEGDKEIQSESVGSHSVTYSTSNKTIEQKAYDILKMYLD
                     SDLLYRGV"
     gene            19605..20039
                     /locus_tag="KRH39_001134"
     CDS             19605..20039
                     /locus_tag="KRH39_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004461219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511053.1"
                     /translation="MFFKDNITLYNACYNKEFGVNEYFKTYLVGVDWQGQQNINVTDK
                     GIISADSVKIIIPFLVASKNKEYITPKEFERLDNAEKEKYFTFNNNDKIVKGLIDFDI
                     DSKNGKTIKILENLYSDVININSIITHDFGSLSMRSWEIGGI"
     gene            20039..20407
                     /locus_tag="KRH39_001135"
     CDS             20039..20407
                     /locus_tag="KRH39_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBG8511054.1"
                     /translation="MSNNNSSEIKINIDNPDKILLKRHLNKNGDAQKLFTNEIRRTTD
                     PYVPFNTGMLKNNVITKVDEIVYKSPHAKKQYYKNKGFGKQGIARGGLRGKQWIPRSW
                     ADNGKNVIKTIARFVGGEVK"
     gene            20404..20838
                     /locus_tag="KRH39_001136"
     CDS             20404..20838
                     /locus_tag="KRH39_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol resistance protein"
                     /protein_id="HBG8511055.1"
                     /translation="MTIIESVRNFIRKCPHLDEFAKGINIEFLNDNVTSYSIETVPAD
                     SILKKFVNGDCIKQFVFIFASIESYGNDILQNIENSDFYEKFAKWIETQNNLGELPEL
                     DGLKESLKIEVTTPSYPFQTDIDKAQYQIQLRLKYFEKGEIN"
     gene            20838..21614
                     /locus_tag="KRH39_001137"
     CDS             20838..21614
                     /locus_tag="KRH39_001137"
                     /inference="COORDINATES: protein motif:HMM:NF037320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511056.1"
                     /translation="MAGVKQRRSIANYLKIEETFYLMGAGFTELNESPSAQTTSKRYV
                     NDKSATKGISGYDWQTAFTTDIIASEKAIEHICNIGKYQLTGSDAETEYIIVDLDEAL
                     SVSEGDYRARKFNIAIEVADFSDEDGEMTASGNLLGIGDPVAGKFNTKTKEFVEGNFE
                     YPPKIQELNITSAEGENIGDTKITVTPVITEGNSYKYKLGQNLALPGFEEVCGAEYII
                     WDGTSDITATKDDKILIVEVNSKNRVLKAGIATVTVKEEL"
     gene            21615..22001
                     /locus_tag="KRH39_001138"
     CDS             21615..22001
                     /locus_tag="KRH39_001138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511057.1"
                     /translation="MSIKFERKNSIMDMEILGEKFEVDFSRDEIAIVLAEVSDNMTNI
                     EKGLDIEEGTIKRATTEILEKLKEEARRGINIILNDDTACERMFKDNNSMALHEEVYD
                     FLVTQYSKYKEKRFEKYTPNRAQRRK"
     gene            22005..22619
                     /locus_tag="KRH39_001139"
     CDS             22005..22619
                     /locus_tag="KRH39_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage Gp15 family protein"
                     /protein_id="HBG8511058.1"
                     /translation="MNSNILIDVLPEHIKIDDKVYKINSDFRTSMIFEMMINDDELSN
                     EEKTNIALNLYYPRIPHDRNLAIEAIIWFYGCGKERKVNGENSRARNSSKKNNRIYDY
                     ELDSEYIYSAFLDQYGIDLQDIKCLHWWKFRALFKSLKEDNQIVKIIGYRSIDITKDM
                     SKEQKDFYKKMKKMYEIPKSQTEKEKINEIEKALLSDGDLSDIL"
     gene            22690..22893
                     /locus_tag="KRH39_001140"
     CDS             22690..22893
                     /locus_tag="KRH39_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBG8511059.1"
                     /translation="MGFNFKKSINLGCGLKLNIGKKSVGISAGTKGARVSVNSKGRKS
                     ATVGIPGTGISYTKTSTKKRKPK"
     gene            22984..25620
                     /locus_tag="KRH39_001141"
     CDS             22984..25620
                     /locus_tag="KRH39_001141"
                     /inference="COORDINATES: protein motif:HMM:NF021648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBG8511060.1"
                     /translation="MADGSIILKTLLDTAGIEKGLGGLNGVAKKGAKLAIAGVAGIGT
                     ALGATTVKAVKFTDEYTKAMNGFEASTGEANAKVNGFGEAMQNVYKHNFGENFEDIGN
                     AMSSIKQNLDGIDASNIEKVTESAIAMRDTFDIDINEGLLGVNSMMKQFGMTAEESYN
                     LMAQGAQNGLNQNGDLADQLAEYSVYYSDLGISAEEMFNMMANGAENGVYQLDYLNDA
                     VKEFGIRTKDGSAGTKQAFADLGLNADELTAKFAKGGKDAKEAFMEVTTALGNVEDDV
                     KRNQLGVQLFGTKWEDLGEDAVKALTDMDGKISGSVDALDKINEIKYNSFGEAIEGIK
                     RQLEVGVLLPLGEAVLPLLNEFANWFAKEGVPQIEKFSASIKDKIPAIATTFKVAFKV
                     LSVLLTAFVRNINIIIPALGLLGGAFAVFKAVDKVQKMVEAFKQFKAITQAVSVAQKA
                     LALVMSMNPFTIALVAIGLLVAGLVHLWNTNEGFRNAVITAWNNIKEVAMTVFGGICK
                     FFTEIIPNAFTSFVGFLGSAFSGLADIAGNIMAGLRDGFVNGWNSIVAFFTETIPTLI
                     GSIVGWFAELPGKIWEGLSAVVTKIGEWGTNLKNKAVEVGTNFVNTIVKFFTELPGKI
                     WNGLVIVVTKISEWGTTIKNKAVEIGTNFLNNIVKFFSQLPAKIWNCLKQVVTKIASW
                     GSSLASSAKKSATSLVNAFINTVKTLPGKFISIGKNIVTGLWKGISGNIKWLTGKVGG
                     FANSILKKAKSVLGIHSPSRVFRDIIGANIVKGVGVGIDVETPSLEKDMDNNLSSLAN
                     RLKATVDLESTKATQSIAVATNYTAEKETQKKNNNVNEDTNSILGGFNLNIENFNNNR
                     EQDMDDLMRKASLYLKRHGILV"
     gene            25633..26358
                     /locus_tag="KRH39_001142"
     CDS             25633..26358
                     /locus_tag="KRH39_001142"
                     /inference="COORDINATES: protein motif:HMM:NF017520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HBG8511061.1"
                     /translation="MFFIYKNRNSKEFGIKIKKINDLSTPKRSIEKKSVPGRNGDLVI
                     DVGNYENFTLSIECDLDSRDIDIENLVTELIDWLQKDFTYEKLYIENRNYYYEAYCSN
                     ALEIERMYKNFGEFVIEFDCKPFKRIVNYSDISIDTKDIDKFSVFNSYDISYPVIEIF
                     GNGDISLKINEQDLILKGVKDRIIIDSDVMNAYKIDAVTKKLVNENSKMFSEFPTLTH
                     GENKITWEGEVNVIEIKPNWVML"
     gene            26388..29285
                     /locus_tag="KRH39_001143"
     CDS             26388..29285
                     /locus_tag="KRH39_001143"
                     /inference="COORDINATES: protein motif:HMM:TIGR01665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG8511062.1"
                     /translation="MSKLDNIKGNLKLYDGNEIDFGHNGLGILKDATDAQVTEEINGI
                     FELEFTYYVDSFLFEKLNYGMIVKADASPGLRGQLFRIYYISKELMGTIKVKAQHISY
                     DLRENFIEKVEFEKCTCEQALNALFRGSTEQNGFVGHSNLTGEYSFSIECVNTYEAIQ
                     GTRGSVLDTFGNGAKIKRDNFNIYINDSIGESNNVLIAYKKNLVGLEAEYDIQDVVTM
                     IYPYAQVDQQSDDTTESVKITLPEKYIESENIRKYPTRKIAYVDFTGEDVTNVNQLRN
                     KAQKWFYNKKDLPSVNYKVDFIDLSQTENYKDYEILETVNMDDEVIVRDLRMQINATA
                     RVVKTNYDPIMKRYNSIEIGNLVNHFAGFKDRLNSIENTLGKVHDDLDDFEVDNGKFP
                     DTLPQTSTLKANGLFATVQLEWSYENKNYYTYELYASQLKDFVPDTITFNNRIYSGQA
                     SAFLHEVKPVQTWYYRVRAGNTHGRFTEFSDQVGASTRKIADGAEYFEEVAIGHALIR
                     DLDVDKVVAGRLKGTYIDAKNLSVTDGNGDITFLVDSFGNVSIKATNFTLKGKTLDDI
                     IGTEIDAITQLEIFNKLTNNGQAKGIYLVGNELFINASYIKSGTIEGTYINAKNLTVR
                     DNSGNVTFAVDSNGNVTIRAVSFSLQGQTIEEIATDISTPIAEREAESAVEAQTQRDI
                     FNKLTNNGQTQGIYLENGRIYINASYIQSGTIACDRITSSSSNPFILLFEGNGAKCAI
                     DASAQYGEGIGKAIRLKYNDNAYLYVSNDALRGYLNGVEIFEFGERNGKSYIHTGLNT
                     QHINPQEAGTYNCGSNNKAWDYLVCNNLNQLSRTTSSNTPYRMVNTRDNSSIVENCID
                     FVRNSVVHPNIFTPYRLKTINENNIEHRLQVDIESNLENPISRYIFKDVSEEAGEGVY
                     AQDITSHLAVLQLSLQKTLNDFENYKNETNNTIKELANRIEALEISTVNKN"
     gene            29297..31186
                     /locus_tag="KRH39_001144"
     CDS             29297..31186
                     /locus_tag="KRH39_001144"
                     /inference="COORDINATES: protein motif:HMM:NF013550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511063.1"
                     /translation="MEKCIEEKEPLANKDILNDIINVVKNLEDEIKKIDDAILKIPPT
                     SDLKGEKGEKGEKGDPGEDLNIKGSFDSIDELPADATPGDGYIIEGNLYVWSNNIWVN
                     TGNMKGEQGDKGDRGIKGDKGDKGDKGQNIKIKGVLNSIDELPTSANLGDVYVIDRVI
                     YIFNEKWLEAGNVKGEKGNPGDRGEKGEKGEKGNPGEPGSDGAKGGKGDPGEPGTDGT
                     KGGKGDPGEPGIDGTFDESKRFSTLITANKTIINATNEIKRNAGEANQFLKNGKGMLA
                     NALTDKNVPTLSTDSFEVIAENIGKIKETPDIPKDSEGVVIRKDSSDIIHKIIKLETN
                     VTEGEATPNELWTNTNNPNWIFYINVDNEDNIIYTTGNMVKKITTENVLVWETNELGA
                     SNLSTVCINTEGKILVASSNGYIGVFNNEGTMLKRIAGNTRQILTVNTDSENNVIYAT
                     FEKNIKKITYEEALIWTFTGHTGYIRGIDIDNKNNIISGSSDKTIRKITNTGEEVWKY
                     SGSTGEVSFVKIDYQGNIVNGNSDATGKITPDGIEIWQYKDAWRGTSILDVDYENNVY
                     IGGADGLLSKLTPSGTLTWKITWGNGIYAISLDSKENIIVGDSGNIIKKMKNTHTVEN
                     IAYFE"
     gene            31204..32586
                     /locus_tag="KRH39_001145"
     CDS             31204..32586
                     /locus_tag="KRH39_001145"
                     /inference="COORDINATES: protein motif:HMM:NF013550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HBG8511064.1"
                     /translation="MRDTIYIVDINNSLYQVATFKQFDNDIDYKIRLTENGVDSDLAG
                     YTVKAFFMDSKKRVFQRNCTIEGSIISTKLDNNILSCAGITTSEFVLYKNDMAVATYP
                     ITLNVVAGIDRDEAIKNIPDWEIIKDIVGIKDIINGKLKELEKAIENIPTKDELVGEK
                     GDKGDKGDIGKGLNIKGSFNSADELPMDAVLGDAYMVQGNLYVHNGTNWIDAGNIKGD
                     KGETGDKGDKGDKGDTGDMGQGIKIKGVLNSIDELPVDAMLGDAYIIENFLYIKGDEW
                     VNAGNTKGDKGEPGLKGDKGEKGDKGEPGTPGQPGAKGDKGEPGIPGQPGAKGDKGDP
                     GTPGRDGTFDETMEFSSLETTSKTLIPAINEVKTEIDLVKQSASSGKELVATAITGKN
                     IATESSDTFEVMAGNIRKIKEMPAIPEDSEGIVIRKDSPSVIHKITKIEIVTKDESSE
                     HIVEKKAYYI"
     gene            32607..32939
                     /locus_tag="KRH39_001146"
     CDS             32607..32939
                     /locus_tag="KRH39_001146"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511065.1"
                     /translation="MQKYLTGLEHKEGDIYKVNLVHYMPFDTKNGLGKTVEELELNGV
                     LVDEITEVEQKEGFSTTMYVNKKTKEITYEYVEIPLTTEQQILKSLKNLEQDNANINY
                     SLMMGGLI"
     gene            32939..33076
                     /locus_tag="KRH39_001147"
     CDS             32939..33076
                     /locus_tag="KRH39_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBG8511066.1"
                     /translation="MLTFDKIKYYYENQMWNEEMVKNAVLKEKITVKEYKEITGEDYK
                     A"
     gene            33153..33416
                     /locus_tag="KRH39_001148"
     CDS             33153..33416
                     /locus_tag="KRH39_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511067.1"
                     /translation="MEELITQLSGLGAVGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVEMGAMKTDVEEIKEDVTDIKDMLQKEGL"
     gene            33417..34223
                     /locus_tag="KRH39_001149"
     CDS             33417..34223
                     /locus_tag="KRH39_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8511068.1"
                     /translation="MKVAIVPGHTLTGKGTGATGYINEGTENRILMDLVVKWLKKGGA
                     TVYSGKVDKSNNYLAEQCQIANKQNVDVAVQIHFNADHTTLDKMGTETIYKTNNGKKY
                     ADRVNKKLATVFKNRGAKSDVRGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKTIDNKENGEGKIMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIK
                     DYVPGQSENLYVVGGGACEKIGSITKEKYTMIKGNDRFDTLYKALDFIDR"
     gene            complement(34292..34729)
                     /locus_tag="KRH39_001150"
     CDS             complement(34292..34729)
                     /locus_tag="KRH39_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511069.1"
                     /translation="MNAKLNKELYNLGFKIGSENVLSRNKILELNDAIYRYQDRNKAF
                     EIILKAFMKLDIPMPAEIIENLDNYEVIVNFVVGVYNGYIEQLNNFSNFISLSDASKK
                     YNKAESTLKQNIKNGKFVEGVDCRLFGKSWVFNIDSLEREYSK"
     gene            34902..35198
                     /locus_tag="KRH39_001151"
     CDS             34902..35198
                     /locus_tag="KRH39_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511070.1"
                     /translation="MKKITKKEIIEFVRDVVEEYRDWKLEKCGFYIKDNELNSFVSFE
                     GKGIDINVYKENYDEIIYIEDYIKDYKRKEYNLKEIDSIIYEDVNEMINNYNEK"
     gene            35239..35709
                     /locus_tag="KRH39_001152"
     CDS             35239..35709
                     /locus_tag="KRH39_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511071.1"
                     /translation="MNKQKARRFLRVIDMNMDKIEEEAIKAFKESCLIKEENDIKIFI
                     DFQGKVEAIAIQAYAKLLDDNKEINIFTSRQIENNLDDMFGEICYVNDYEEFVNWCED
                     KCENLNWDSYKKFNKENFEEIAERNIDDSTPVFLEELREGIESCKQELQNIVEN"
     gene            complement(35945..36226)
                     /locus_tag="KRH39_001153"
     CDS             complement(35945..36226)
                     /locus_tag="KRH39_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511072.1"
                     /translation="MNVYLQIGKDLSEKMTKSELNKFSQEVVNFLRYAGVDKDMKIKL
                     FQTIIEAVARKKITLPNDNKFTEGILQSSEFEMTENVGFILEGLSYRNN"
     gene            complement(36370..36705)
                     /locus_tag="KRH39_001154"
     CDS             complement(36370..36705)
                     /locus_tag="KRH39_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511073.1"
                     /translation="MPKFDEELLSNKEKPVFKKNGTIIQNNSTTTDNKTLNIYNLVNK
                     KSKKVTMSATLDEELVNKLKSFSIDMNSDVSKLLSDILTQILSDVTIKEENLNIYNER
                     NRRNKTKKK"
     gene            complement(36706..37518)
                     /locus_tag="KRH39_001155"
     CDS             complement(36706..37518)
                     /locus_tag="KRH39_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG8511074.1"
                     /translation="MANTKLLTYFNIKGGIYKTTTSIMTAYELAKDKDKKILLWDLDV
                     QANLTQYVYKVNHNDKTTLDILKNCSADDIIIKSFNKKYSNVDLIASDIQMTLLEENL
                     FNFKDDKEKILAKWYMENFDTFSKYDYIICDLSPHYDLIAKNVLFLADSIIVLIQDKN
                     VSSLRGAELFKQLWDADRALLDKEDNIKNFVLVGFEKKKTQVSEVFDSYLEGFSELRS
                     MMLKTYIRKNEFIEKALMKKLSLTDFLKDSKDHFSRQEFTNMLEELKVKGVL"
BASE COUNT        14933 a         4421 c         7229 g        11034 t
ORIGIN      
        1 tatattatat ttacaatatt aaaatattgt atatacaata ctaaaataat atttttataa
       61 tattatagta ttatttttat attatagtaa aattttaaaa agaaggctaa gaaaaaagca
      121 accttctttt atagaaaaaa agtcattcat tcttagcctt ttcaattatt atcctgtcgc
      181 cctcgaatgt tgccacaata ttagggtttt ctcttgttat gcccatttca tcagcccatt
      241 ttttaggaac actcattcta ggagtaaacg acccactccc acctttatga aagttaaact
      301 ttaaatctct tttttctttt ttcattcatt agctcctttt ttgctgtatt acaaagaaaa
      361 gtaataatac aacactaaaa actaaaccga gtatgttgct tgtactagag tttaatacta
      421 tagatactat atttaaaact atagttatta aaactaaaac aatgcaaatc ttataaaatt
      481 tatcactcat gtttttttag aaatgtgtta taatattaga aagaagtcac ctagagagaa
      541 gggctattct ctctaggctt atccttacaa ctcttttaaa attcttagaa ttgcaaggat
      601 tatgttaagc aataatagga tgaaggagaa aactttttta actatgttaa atttgttttc
      661 tcttttcttt ttacttcttt ctaatctccc cattcctgtg cctcctttct tttatttttt
      721 tgctttctca cctccttctc tataaatata ttataccatg acggggcgtc aaaagcaata
      781 gttttctcct attttttaaa caaattttgg taaaaagtaa agattttagt atattagaaa
      841 tgatgtaaaa ttgtatcttt taaagaaaat gtgatataat aaaaacaagg aaataatgat
      901 ttactttaca caagagtagc tatttccaat cagattaaaa ataacttatt ttcttaaacc
      961 actcttattg gcgtttgagt gttttttaat tttatcgttc ttaaaataaa ctttgacaaa
     1021 aataaaaaaa tagaatactg aaaatgatgt aaagttgtat cttttaaaga aaatgtgata
     1081 taataaaagc aagagaactg tattctagtc gtaagagtag agttttcgtc taaagtaaaa
     1141 aattatctag actttttgaa tttgattttt aaatcaaatt cccaaccact cttttgcgca
     1201 gagtggtttt ttacttgtct aaataattta ctaattatat agaatattaa actagctata
     1261 acaccagcta atatgttaag taaaaaatta tccatacttc atcacctccc ctcaaaattg
     1321 ttgggaggat aatcttttgt gtatagactc cactcttttt tgaacacaga ttgcttttct
     1381 cttgctaaaa ttattataac atatatttac atataaaaaa gacttagtaa gtgtactaag
     1441 tcttttaaaa tacagttttt tagaaaatat atttttaaaa tataatttat tttcaaaaag
     1501 agtatatttt tttataattt aaatgtgcta taattataat cataacaagc agaaaacttt
     1561 tttttaattg gtttaaaaaa atctacaaaa aataaacaag gaacatgtac ttgccgtcaa
     1621 acaaaccatg ttccttacac cggaggttag acctctctta aggataatat atccttgctt
     1681 ttctatactt atatttatat tataagacaa agtgtagaaa aacacaaggt ttttagggta
     1741 attttaatag taaaattatt acaaaaaatc aaaaaaagag cgtcagcctc caggtgaaat
     1801 tggaggggta aagatgaaga atgttaataa aaataatata gataaattat ttaaaaatga
     1861 ttgtgactta gatagagcag taggagaaat agaaaattac gcaaagttcg agagacaaga
     1921 aacgcataaa ataaaaaaat atatagatga aaaaaagagt ggagtatata atgcgatagc
     1981 gagagcagga gcaggagaga cggttctttt acttgctgaa acagggacag gaaaaagtta
     2041 tagctttata aatgcactta aaaaaatgaa tacaaaagca ctattcatat taccaaacag
     2101 tgcaaatgtc gagcaagcta tgtgtgaata tgacatacca ggggcatacg atttgatgcc
     2161 agcccctaag gcattgaaag aaggaaatct atcagttttt acttgggata aaacagaaca
     2221 attactaaac attgatttga gcgattacat aatcgttgtt gatgaaatac atcaaacgta
     2281 tacagattct tacagagaaa aagcgataaa aaatttaaat aatatagttg caaaatgcaa
     2341 aggaagaata gatataacag caactccaac caaattagag tttgaaatat atgactacat
     2401 aatagaatac ataaaagaaa aaaatactaa atatgatgta aaactttatg acaatataaa
     2461 cttagataaa atgatagaga ttatcaataa atcaaaaaat agtgcagttc tttataataa
     2521 taaaaaggat ttaaaatata taaatgaatt aacaacaaaa aaaggtgaca tagtttacgc
     2581 aaaaataaag gctaaaagtg aattgtacaa gagaataata cagcactctg atatgggaga
     2641 ttatgaactt ttactaaaca caacaacaat tttagcaggg gtaaatataa aaaataaaga
     2701 tataacagat ataataatcg caggttttaa agacccagca acgataaaac agtatgttgc
     2761 aagatttaga gaattaaaac gtgtaagggt tcatattttt aataatttta atgatacaga
     2821 ggcaagtaac aaggtttatg aagttaataa tataattaat attaatttta aaagagcaaa
     2881 taaattaaaa gcattatata acgagttaaa tagtaaaaga gcaacaggat ttgaaactgt
     2941 tgggatagac cttgttcctt ttagtataga ttctaatgta tactattcaa ctaaaaataa
     3001 atgttatgaa atagataagt tatttattat aagcaatact tacacaaggt attataacag
     3061 cagaacaata gcgagtttaa aagtactttt agaagaatat ttcaaagatg taaaaataga
     3121 aaacatagaa gaacaagatg aggaaacaaa gcaaggaata aaagaatata aaaaagtctt
     3181 aaaagaaatc aaaacagcaa cattaaaaac tttagaagaa aataaaaagt acctagtcgg
     3241 ctatgagcaa attaaaaggg gttctaagga ctttaacctc atgagctata tgaattataa
     3301 taagatgtct gaaaacgact acaagcaggc atacattaag tataatatag attatttagt
     3361 cgacaaagca gactgttaca gcgaaattga actattttct aaatatgtac tagaacataa
     3421 ctatagctta gaattatcgt ggaaacttgc taacatgggg aataataaaa gaggaaatat
     3481 atttaaaaaa ataaataatc ttatatacag agagttaaaa gaagaatacc cgaacctgat
     3541 aaatgataac ttgatagaag ttgttgcata taactatata gataaacttt ttggaattgg
     3601 aacagcttac acagaatata acttacaaga gttaagtaac gatttagcga taattttggg
     3661 ggataattgg agatttgcga caaaaaaatt agggaatgtt ctaaagaata tatttatagt
     3721 aaaagccaaa caacacagaa actttgaagc attagaaaac tattcatatt acaaaaataa
     3781 taagccgaat actgatttta tagacagtga cagaatacga ataaaaaaaa ttgaacgata
     3841 cataacaata gaggacatag aaaaggattt aaaactagag aaaggcgatt taactttaag
     3901 aaatgcaata aaaattagaa agaaaaaatt attagacaac ttagatgaaa ttgaaaaagc
     3961 atttttaaat aaagagttag ataaagaact tagaattgaa agagaaatag aattagtaga
     4021 gcaactagaa atttgcattt aagaacaaaa agtcaccgaa gtaggattta atatatatat
     4081 atatatataa attcctactt cggtgacttt ttgataatga gtcatcaata taattagcat
     4141 acaatattta aagaaatgct ataaaactag aaaacaaagg ctattgagta aattggatga
     4201 aatttaaaat aggaatgtat acaattaaaa gaggaaaatt cactttagtg tagaaatata
     4261 tataaaaaag caaatatagg gggatagaat gaaaaaaata ttattatgtg tcattattgg
     4321 aatgttggga ttaaccttag ttgcctgttc gtcaacagca agtggagata agattgatgg
     4381 aattgaaatt gttgcaaagg aaacatctgc ggaacaaatc aaaggagatt gtaaccagga
     4441 tgggagctat aatgaaaatg gagagtattt ccagatggga ttggatacag ctaaggcgtc
     4501 agattatgaa tataaaatag ttaatgtgtc agttaaaaat gctagcgacg aggctattaa
     4561 actgtttcaa acaggctgga acgctacagc taaagacggc tacgaatttg aacatataag
     4621 ggttacagat aagttggaaa atcaacaagt acctgcaaat tattcttttg atgcacaagt
     4681 taagatactt gttgaaaaga aaatgaatgt aaaagagata atacttaaat ataatttaaa
     4741 agactatagt aacttaacta atgctatgaa agacgcgagc tctggagcta ctaaagagga
     4801 tatacaaaag aagtatccag aattgtatga cgataattgg atagaatttg gaactataaa
     4861 atttgaataa aaaaggaaaa ggcttaatgt tttgcattag gtcttttaaa tatagtaaac
     4921 agcattgtta tgttttaatt tttgtggaat tattttccag tagaatgaat ggcgaaatag
     4981 tactataatc gtagtataag gtataatttt gtcataaatc gtacatatgt tcttgaaaat
     5041 aatacaaaag gggagtataa tatttgatag ttacatactt gatacaatta ttatcttatt
     5101 atttttttca cagggggatt gggtatatga aaatcgaata tattaacacg gaagaatata
     5161 ctgaatttat tgaatttatt gaatttattg ttagtgctaa aaaaactaaa atgaaagaat
     5221 ttatatacat aaaaaaagtt ctttttgaat tagaaaaaga agatagatag tatctatctt
     5281 ctttttttta ttcagagtct aacgattttt gcattaattt ttttatatgt tctcttttgc
     5341 ttggcgttaa ttttaaatat aattttgcca tttcctcaat ctctgggtct aaatctaagt
     5401 caattagtgg gtctataaat ttcttaccta ttccattcac tagccaatcc ttattcacct
     5461 tgtaaactct gcaaatatca tctattgccc tgtccgttgg tattctatac cctttttcgt
     5521 aagaagcaat ctgactttga cttaaattta ttttttcgcc aaaagccttt tgtgatatat
     5581 tttcaaaact tcttatttct gcaactcttt cgcatatgcc aggtatttta ccctcgttac
     5641 tatttctttc cttcaaagta aactcctcct tttgcgatat tataacatac ataatatcac
     5701 aaagcaatgt aatgcgattt aaaatattgc aaaaatatta tttttattat ttagtgataa
     5761 atagtattgc aaagtaataa aaagtgtatt ataatctttt tatagaatta aaaaaaggag
     5821 gggcaaatat ggaatataac ataccatatg accaaaaaga cttcttagaa tttcttaaat
     5881 taggtgagac tatcatgggg caaaatcctt tgaaatttca aagattaatc ggacgcatgc
     5941 aaggaatagt tgattgttta gaagatgaag aggagcaata aatgctaatt aaataaaagc
     6001 gtactttgac ggctacgcta aaccaattaa aaaaagtaaa aaataaataa ggaggctgga
     6061 aaatgataaa agttatttta tgtagtacta tcgacgaata tatggagtat gaggacgtaa
     6121 taggagcaaa ggacagctat ttaatggatg ttatcatagg tggcgaatgt tcagaaattg
     6181 atgctataga aactttagaa attttaaaaa atagtaaagc gagaattgta tcagaagcag
     6241 aaaaaacttg tttaaaattt ggtattggta atcaaatttc aaaggcgata gaacaaggta
     6301 taaaagaggc tccaaaacta aaaaagggga tacattgtga taattgtggg gcaagcgtag
     6361 gcagttcgga ggaattaact tcaatgccag ttttagaaaa ttataacaac aatcaatccg
     6421 tttctgacca actaggaagt atagacgtat gcccaagttg tttgctagaa ataacagact
     6481 tactgtaatt ccctgtttta gttgaaaaga aacaaaaagt aacagcgtaa gccgtcaata
     6541 aaaaaaagaa gggaggattt aaaaagatga ttaatttaga aacattgtgt aatggagaaa
     6601 caaaagaaaa gattgaaaat gggttcatgg agatatttaa aaacatacaa gaccctaata
     6661 caccagcgac atctacaagg tctttgactg taaaggttac actaaaacca ggcaaaaatc
     6721 gttctcatgt taatacacaa atacaagtta ttccaaagct cgcaagtgtc ttacctagtg
     6781 aaacagacat tattgtagaa aaggacttta ggactgggga agttaatgca aatgaatatg
     6841 gaaaccagtt gccggggcaa ataaaattag gcgacttgga gagtaaagaa acaagtgtaa
     6901 cagaagaaac agaagtaaaa gaaagtgaag aagataatat tcgtaaattt aagagtttaa
     6961 aagacttata aaaactaaaa aggagtgttt aatatgttaa cagatttatt taacagggta
     7021 atggaagttg gagcaagaaa aatagaaaca agagaaataa atggagttac atatacgact
     7081 gaccgtttaa ttccagtata tcccaaaaca gttgaggaaa ttgaattaaa tacattagac
     7141 ggtttaatag agtatataaa atcagaggtt gacaaagatg caaaaggcaa tagacttata
     7201 gttcaaatta aaagccctaa aagtgttaga ttatgttctg aactgctgga aggtggtaaa
     7261 aggaatactt atgttgattg caaagcgtta ttacctcata ttaattttga ttgctcgctt
     7321 gattcagagg aatttaatat tatgctacaa tcttcttttg tagatacgga agataaaaat
     7381 ttgcttttaa aagtagctgg gaatgtaaaa gaaactagcg taaaaactgt acaggatgat
     7441 ggagtatcac aagtggttgc aatgtctaca ggtgtagcaa gtgtagagga tgtaatcctg
     7501 cctaatagag ttagtttgaa gccttacaga acttttacag aagtagacca gccagagagc
     7561 gagtttattt tcagagtaaa agagggcatt acatttaagt taatcgaagc agatggtgga
     7621 gcatggagac ttgaagcaat aaagaatata aaagagtact tagagaagaa attagaaggt
     7681 attgaaaata tagatataat agcataaaaa aattgaagag gctggactaa ataaccagcc
     7741 tcttttaaaa aaggagtgtt tattttgtat aaagatataa caaaaatggg ggcaattgaa
     7801 cgtggggaat acgttggtaa tgttgctctt gaattaaggg aagaaaatcc tcatcttagt
     7861 aaaatagatt taattgagtt agctcttaag aaaataagag aggaaagtga acaggaggct
     7921 gggcaatgaa aataggtaaa tatttaatcg tttcccagga gcaggtggat agaaatattg
     7981 aatgtttgag agaagaaaac aaagtaaggg acgataaaat aaagttttat gagcgtatta
     8041 tgagtaacaa tgaagaattg attcaaattt tagaagaaca gttgaaagaa tataaaagtg
     8101 aattagatgt atatagcttg tttatagatt gtattttaaa taaagaaaca aggaaagcga
     8161 tagcgattta taacagaact aaaagcatta ggattaagca gaaatgtctt aattctatac
     8221 ctataaaaaa gggttaagtt aaataaataa tttccttgta ggaataagag aggtatttag
     8281 taaatgaaac gatataaatt ttatattgat gaagtgattt ctttgaaaca taaagttatt
     8341 attgaaattc ctaacgaaat gagtgctatt gatatttctg aaagtataga aagtgaatgt
     8401 aatttgataa gtgatttagc tggatgtata gaaaaatttg gttgcaagca aattgatttt
     8461 gcggaagatg tgcatggaaa aagagatata ttagtaaata cttgtaagaa aattgaggat
     8521 tctaaaaact agaaaaaata taagggagct taaatatgag tgaatttgtg cagtgttgtt
     8581 gctgtgaaag aacaataaat atagaagaaa ataactatgt acagtatgaa aaagaggctc
     8641 taggtttagt ttttacactg tatttctgct taaattgcgt agatgaatta agcgaaatgg
     8701 aataattttt aaattacgaa tggaggtata tagatatgat aatgaaaaat gttttaaaac
     8761 tattagcaat gtattgcttt tgcccagagt gtggaagtga tgaacttgga gaaggtgaag
     8821 gaagtttaat cgttgatgag tatacatttc atagaaagtg taaatgtgga tttgacgtga
     8881 tagtagatga aagggaggac aaaatataat gaatttttta ggaatgataa ttttgattat
     8941 atttgcattt tttattggtc gtatatatga atatagagtt aatttaagag aatgtgagaa
     9001 ttgtaaggga gaggttggaa atgaataaca ctacactaat tggtagacta acaagagacc
     9061 ccgaattgaa atatatacca ggctcgggaa cggctgtatc gacttttaca atagcagtag
     9121 atagagacta tattaaaaaa gatggcacta aggaaactga ttttatacct attgaggtga
     9181 tggggaagtt agcagaagta tgtgctaata atttagataa aggtagatta gtagcggttg
     9241 aagggtctat aagagttgaa cactatgaaa aagatggcga gaaaagaact tatacaaaag
     9301 ttcatgccaa taaaattaaa tttttagatt ataaaaagga tgatactgga aaggcatata
     9361 tgtttgagcc gggaggctta gacccacaag gcttccaggc tatagacgac ggtatacctt
     9421 tttaagaagg aagtggttga acattgaatg aaaagtggaa acaatatata gaaaatgttt
     9481 tagaatcgag aggttattgc ataaatagaa tgccatatgt atattcatat gattttcttg
     9541 taaatagcaa tattaagata aatgtagaaa tatctaatct ttataaaaat gaggaaaatg
     9601 aatatcacta ttttgaatta gaaaaagaat atcatagttg cgatatttat atccttattg
     9661 cacttgatga agtcggaagt gctttgaaaa tattagtaat accagctagc aatttaatgg
     9721 gacaaaagca aataattatt aataacaaaa gtaagtatga taaatttgat tcaaggtttg
     9781 aacttataaa ggtatatgat aatttttata atgaccttga ttgtgttatg tagaggtgtt
     9841 ttaggtgttt aaaaaagtag aaaaaaaatt tagtggttca gttaatccga atttcatgga
     9901 agatgaccta acttttaaaa attatttagc gttatattgt tgcataaaac cggttaaaat
     9961 tcatggaaat aaaaaggttt tggctagttc cactaaggca tgcagtgcac tgaaaattag
    10021 agaacatcag aaaaaagcgc gtaaaaaggt aaaacttata aatgcaaaaa caggtgaaat
    10081 aaaagaaatg gctatagatg aagcagaaaa ttttttaaat gttaaaaatt tatacgctgt
    10141 tatttggcga gggagaccaa ctcgaactgg ttggtatgta aaggatgttg gaaatgaagg
    10201 agattaaata taacgttata tatttcaatt cagaaacttt aaaaatggat aagagaagtt
    10261 ttgacgcttt aaaagatgct agagcattta aaaaggaaaa agagaaaaaa tacaaaaatg
    10321 ttgagattat taaaaagaca attatagaaa aattgataat gtaggaagtg aatttatggc
    10381 aaaaaagaaa aggatttgtt catggtgtgg taagtcgttt tttacagagt cgaaaaatag
    10441 attttgctgt aaatcatgcg aaagaaagta taaaaaagac aaagaaaaaa acgcaagttc
    10501 ttgcttagtt gaagaaacca aagaatattt aattgaacta ggaaataaga aaagaagagt
    10561 agatattcta agaaaagaga tagatttttt aaaagataaa gagttttata gagagataaa
    10621 tttcaatgaa ctagggttta aaatacattc tagcccgaag ggcatagatg aaatgattat
    10681 gaataatgag gataaaatat catttaagga aagtgaaatt gattttatag aatatagatt
    10741 gagactaatg gatatttaca taagtgaact tacagaagag aagcaacaaa ttataaaatt
    10801 tatgtatttt tatgatggcg caagtaaact ttctaatgaa gaaattgcct caaagattaa
    10861 atgtagtcgc agtaatgttc attataaaca caaggaagca ttgaaagaac ttgccgaaat
    10921 gctttttgaa agtggaagtc ttttgtagaa caaaaaaaag acggttttta agacaaagtt
    10981 agaacactct tttacgttta tatagtgtat aatattaata tcaagaagtt taaaattaaa
    11041 tagatataga tttgaagcct ggcaggaatg ttgggctttt tataatgcaa attaatattt
    11101 taagggggtt gagaaggtga atagaagtgg gtagtaagaa accagctaac ccagtcaaag
    11161 acaaacgtat ggtcttaaat atacaagaat acttaaaaga aaagaatatt agagattatg
    11221 ttctatttgt gctgggaata ggtactggct acagggcagg ggatttagtt aagctacagg
    11281 tgagggatgt tagaaatgca atagacgaag gttatttctt aataatggag agtaagaaag
    11341 aaaagactaa gaacataaga aagaaaaata aaaaacctag aaaagctcca atagttccta
    11401 atttagaaag agtactcaaa agctacatac gagataaaaa ggattatgaa tatatgttcc
    11461 catccaggca aaaatctgtt actccgtata taggtgttga gagggtaaca gtgatactga
    11521 aagaagcagg tagatatttt ggacttaagc atataactgc gcatagtatg cgtaaaacat
    11581 acgcttatac catatacgaa gaaagtggat ttgacattat aagagttaag gaaatgcttg
    11641 gacattctag tatagaagaa accaaggcat atctaggtct taatgaagaa caatatcaag
    11701 aatatagcat gtttttaaat gacctaatag ggtgattttt ttattttgtc tttttgaatg
    11761 ttggaaaaat aaagacctgt acattcaatg tatttttttt gctatatata aagtaggaaa
    11821 agtaaaaagt gaatgtctga ttctctaaga aaaccccata ttcgttaaaa taggcgttag
    11881 ccattataaa tactgggata gaagctattt aacattaaaa tggccatgac gaagcttaag
    11941 gacatgcgga caaaacttaa aggacatgga aaaatagaga atgagtttga tgcaataaaa
    12001 actacaaaat tcacaggcaa acaattcaat tacgcccttt tacaagagta atgacaatac
    12061 ttataagtac ttttgattca tttaaaaaaa ttcactcgta ttcataactg tacggtattg
    12121 actacgattt aattatgtag taaaatgaac atatagaaag tagtcataac taatggtcaa
    12181 aagtaagggg tgtattttta tgatatttgg gtattgtcgg gtaagtagta aagtacaaat
    12241 tgataataat agtttagaac agcaggagca ggaaataaaa agacattata gtgatgctaa
    12301 aatatttagg gaacaacata cagggtcaac cacgcacaga cctatttttg aggaaatggt
    12361 taatcaaatg cgtgatggtg ataaattagt tgtaactaaa ttggatagac ttgcaagaaa
    12421 tgcaacagaa ggcattaatc ttatacaaaa tttatttgat agtggtattt ctgttcatgt
    12481 gcttaatgtt gggcttttag aaaatactac tcttggcaag ttttttataa caaccttgct
    12541 tgcagttgca gaaatggaaa ggaatctaat tttagaacga acttttgcag gtaaggagat
    12601 agcaaagcaa aatcctaatt tccgggaagg tagacctaaa aaatatacta agatgcaaat
    12661 ggaacatgct ttagagttat taaaaactaa ttcttatacg caagtagaga aaatgacagg
    12721 gattagtaag agtacattgc aaagagcaaa gcgaaaacaa gaagactaga tacctagtct
    12781 ttttttattg aaaggagttg atttgtattt caataaaaaa ttccaatcga gaaaaagcgt
    12841 ttgagattta caaagagaaa aatgggagaa taaaattaat tgatatctct aaaatgttag
    12901 aagaaaaaag ttgcaatatt agtcgttgga aaaaaattga tagatgggat tatagacttg
    12961 gaataaataa gaaagttggc gcaccagccg gcaaccaaaa tgctttaggg catgaaggag
    13021 gcgcaccagt gaataatcaa aatgctagaa ctcatggatt tttctctaag cttttgcctg
    13081 ctagaactta cgagattgtt aaatatatcg agaaggatgg tggtaattct ttagacattc
    13141 tttggaacag tatagtagtt cagtatgcga aaatattaga ttcttttaag attacccatg
    13201 tcaagaataa aaaagaccat acaactgaca ttaagaaaaa tggtaataaa attaaagagt
    13261 atgaaattca acactcttgg gataaagtta caaatgctat gaaagcagag gcgacagcat
    13321 ttaaagcttt gtcaaaaatg ataaaagact atgaggaact acttcataaa aattgggatt
    13381 tagcaacaga agaacaaaaa agcagaatag aaaacataaa agcgagaaca aataagttga
    13441 ctggtaatga cttagaaata gaggatatag aagaaataga ggcagaaata tatggcagta
    13501 actaagaaga aaactatacc ttttttattt ggagataagc acaaagaata tattaaaaag
    13561 tgcgctatca atacatacaa tattgccgaa ggtgctgtaa gggcaggcaa gacggtagac
    13621 aatgtatttg cctttgctca tgagataaag gtatctaaag ataagataca tctagcaaca
    13681 gggtctacaa gtgcaaatgc aaaaatgaac attggagatg ctaatggttt tggacttgaa
    13741 tatatctttc gtggtcaatg tcattggggt aaatttaaag gcaatgaatg tttatatata
    13801 aaaggtattt caactaagca taagcaaaaa attgttattt tcgctggagg tgctaaggct
    13861 gatagttata agaaaataag gggtaactcg tatggtatgt ggatagcaac agagataaat
    13921 ctacatcatg acaatacaat aaaagaggct tttaatagaa ttatagcatc aactaatcgt
    13981 aaaatatttt gggatttgaa ccccgataat cctaattctt ttatttatac agaatatata
    14041 gacaaatatg aggaaaaaga taaaacagga gacttagttg gtggttataa ttatcagcat
    14101 tttactatac atgacaatat aacagttacg gaagaacgta aacgtgaaat tttaacacag
    14161 tatgacgttg ggtctatttg gtataaaaga gatatattag gtcagcgctg tgtagccgag
    14221 ggacttatat atagactctt tacaaataac agggaagaat ttaaaataca agaaaaaatc
    14281 aacgacttga tggaaataaa tataggcgta gactttgggg gaactggttc ggggcattcc
    14341 tttgtggcta cagggattac aagaggctac agggatgtta ttgcgctttc gagtgaaaga
    14401 catttcggag aaatagaccc ggataagttg ggtcttttat ttgtagaatt ttgtttgaaa
    14461 atagtaaatg tatatggctc tataacttgc gtctattgtg atagtgccga gcaagtgcta
    14521 attcgtggat tacgttctac agctaaaaaa aatggtcttg gctggttgaa aatagtaaat
    14581 gcttataaaa gtgcaataaa tgatagaatt aatcttgtaa gtagacttat ggcacaagga
    14641 cgatttaaat atgctgatga ttgcaagact ttagaagttg ctttatgtag tgccgtttgg
    14701 gattctaaag agattactaa aaatgtgagg cttgatgatg ggtctagtga tatagataca
    14761 ttagatgctt ttgaatattc aatagaaaag aatatgaata agtttattaa atatgaatag
    14821 tagggggtgc tttgttgttt tcaaaaatat tgacatggat aagggaagta ttaaagaaaa
    14881 tgacttccga ggcaggaagt aatttagttt ctgatatact tataagttca gaaatgatta
    14941 aaaacattga attgtggtct gatatgtacc aggatagcgc ctattgggtt aataatgtta
    15001 atgtttttag tatgaattta gcatctagta taagttctga aattgcaagg cttgcgacca
    15061 tagaaatgga ttcaaaaata actggaagtg ttagggctga ttttctcaat gaacagttca
    15121 aaaaggttct gaaaaatata aggatacagc tggagtatgc ttgcgctaaa ggtagtatta
    15181 tatttaaacc ttatgtcttg aatggaaata tagaaataga ttatatacat tcagactcat
    15241 ttttccctac atcttttgat gcttctggaa aaattactgg agttgtattt gtagaccaaa
    15301 aaattaaaaa caacaagata tttactagat tagaatctca taatttattg aaagaaggtt
    15361 gtttgattag taataaggct tatgtaagta gtaataataa tagcctcggc agggaaatta
    15421 gtttacaaga tgttgaagaa tgggcagaca tccaggagca gataactatt aaaagtgata
    15481 ggttattagt tggatatttt aaagtaccta tagctaatac ggttgatacg aaatccccct
    15541 taggtgtttc tgtttattct cgagcgacaa atttaattcg tcatgcagat gaacaatatt
    15601 caaggctttt atgggaattt gaaggttcag aacttgcaat aaatgcgtca gaggatttgt
    15661 ttactaaaga tattaatggc aatgctattt taccgaaagg caaagaaagg ctatatcgta
    15721 aattagagat agatagctta agaacgacag aaactttttt tgaaccattt tcaccatcaa
    15781 taagggatgt ttccttaatt aacggactca atgaacaatt aagaaggata gagtttagtt
    15841 gtagtttagc ttatgggaca ttatcagacc ctaataatgt cgataagaca gcagaggaaa
    15901 taaaagctag caaacaaagg agttatgcat ttgtttcaga tgtacaaaaa gagcttagaa
    15961 acgcattaga ggacttagtt tacgctatgg acgcattaac aactacttat aatttagccc
    16021 ctgttggaaa atatgaacaa agttttgatt ttgatgatag tttaattgtg gataataagg
    16081 cagagcagag cattaagttg caagaagtag caagtgggat attgaagccg gaacgctatt
    16141 taatgtggcg ctatggggtt acagaagaac aggctcgtga aatgatgcca gacgatttaa
    16201 tgggtgagta gatgctgaaa tctgaaagat tacaagagct gtctaattat tttatatatc
    16261 tttatgaaca actggaagat tttattatag gagatttttc aaggcgagtt agtaaggctg
    16321 ggactgttac agacatggca gagtggcaaa ttataagggc tgaattgttt ggaatgagtg
    16381 agaaggcttt gaaaaagaaa atttcgagcg ttttaaacat tactcttaag gaagtcgata
    16441 aactatttga agaaattgcg ctagagtcta taagtgctga tagtgcattg tatgagtatg
    16501 ctaaattaac acctttacac ttaagtcaat cagaagaatt aagagattat attaatgcag
    16561 taaaagaaca gactaaaagt gaatttaaaa atataactgg ttctctgggt ttttgcacta
    16621 ctataaatgg cattaggaga aataaaaagc taacagatgc atataggcag gcgttagacc
    16681 ttgcgcaact acaaatatca agtggtgcag ttgattataa aacagctatt aaaatagcta
    16741 ttaaaactat tagtaaagaa ggtgttaagg ttgtaaaata tgatactgat tgggtaaata
    16801 gattggatgt agctgttaga agggcgacac ttacaggcgt tagccaggta tcgcagaaaa
    16861 taaacaataa ggtcatagat gatttaggaa cagatattgt agaggtaaca gcacacgctg
    16921 gtgctaggag tgttggaaca ggaattaaaa atcataaggc gtggcaaggc aaatggtatt
    16981 cattaagtgg taaaagtaag gaatatccct ctttaaagcg tgttactgga cttggacatg
    17041 gggatggtct aggagggtgg aactgtacac atcaatactt tgctgttatt cctggagcat
    17101 ctacgccaac gtacacaagg gagcagttgg aaaatataga ccctccaagc tttggatata
    17161 aaggtaggac atatacgcat taccaggcat tgcaacagca aagaaaaatc gaaacagcta
    17221 taaggcagac taaaagggaa attattgcat ataaaaatgc tggattagag gaagatttta
    17281 caagtgctag tattaagtta aatagacaaa agcaagagta taagaatttt agcagggtgg
    17341 cgggcttatt aaaaaggaat gagagacacc aggtacaaga cttcgataag agcataagtc
    17401 aaaaatctgt atggtccaat aaaaagaata aataagttaa acaaagtctt taaaataaag
    17461 gcttattttt atgtttaaaa actaataagg aggaaatatg aataaggaat ttttaagtgg
    17521 tttagggtta aaggatgata ttataaccca ggtattagaa agtcattcta gtactttaga
    17581 aaaggaaaag gaaaaatatc aatccttaga aattaaggta ggggggttag aacagcagtt
    17641 aaaagacgct aataaggaaa taaaaggcta taaagacatg aacattgaag acatacagaa
    17701 aaaagcgcta gagtgggaag ctaaatataa tgatgataca aaagcgctta aggaacaatt
    17761 agaaaacaag gattatgaaa ataccttaaa ggaattttta ggaaaatata aattttcttc
    17821 tgaactttca aagatggcag tttttgcaga tttaaaagaa aaaaaattaa aaagggaaaa
    17881 tggaaccttg cttggagcag aagaatattt aaatcaacta aaagaaaaag acccaggtgc
    17941 ttttttaagt gatgatgttc cagggactgt catatcaagc acaaagggac tgcaagatgg
    18001 aaaagtttta aataaaaatg aagatgctaa tatagcgtta aggtcgctat taggaaggga
    18061 gtagtaaaat gacaacaaaa atatcaagag aaaatgcaga ggctataatc agagaacaga
    18121 tagttaataa tatattacaa gatacaccta aacaatcaat ttttatgaac ttggcaaaaa
    18181 aaatgcctaa tatgtcatca aatacaactc gaatgagagt tttagacgtt ttaccaacag
    18241 cttattgggt caatggtgat attggttaca aacaaacaac tagacaagcg tgggataatg
    18301 tatatatgca tgcttctgaa cttgctgtta ttgttccgat tcccgaggct gttgttgatg
    18361 atgccgagtt cgatataata ggagaagtta caccaagggt tgttgaagct attgggcaaa
    18421 ggattgatag tgctgtaatt tttggtgaaa atagaccgtc agaatggcaa aatgatataa
    18481 ttacgcttgc taggcaagcg ggtaataatg tttcggttgg ctctaaccct aactattatg
    18541 acttaataat gtcagaaggt ggagttttaa ataaagtgga ggaatgtgga tacatagcaa
    18601 ctggcgttat tgcatctagg ggaatgaagg ctaagttaag aggtttaaaa agtacagatg
    18661 gcacgccaat tttttcaact agtatgcaag gaacaacgaa ttatgcgcta gatggcgcac
    18721 ctatgtattt tccagataat gggtcttttg atagttctgt agctcaaatg attgttgggg
    18781 attttaatca agcagtttat tctattagac aagatataac agttaaaata cttacagagg
    18841 gagttattca agaccctaat acaaaagaaa tagtctacaa tttagcacaa caagatatga
    18901 ttgccttaag ggttgtattt agaatgggtt gggctttacc taatccagca actagacttg
    18961 acgaggatag ggtcggttgt tcttttgctt atttagagcc acaaacacca gtaacaactc
    19021 aaacagtaac tattacagta aaagacaatg ctggtacgcc agccgtcgta gctgatgcta
    19081 tagtagatgt aaatggttca agaatgaaaa ctaatagtac tggaaaagtt gtatataatt
    19141 taagacctgg aaactatcct atgaaaatta aaaagactgg atattcaact ttaaatgata
    19201 atatagttgt tagtaattca gctgtaacta aagatattac tttaatgatt aataaataag
    19261 gtggcacaaa atgaaaacat atattgatta tgaattttat aagagccttg ggggaaaaat
    19321 cccccaaggc tcttttgata gatacgcaat aagagcaact aagtatgtag attataatac
    19381 ttttaataga atagaagaag ttacagaaga aataaaacta gccacttgcg aggttgctga
    19441 cctcatatac aaaagcaatc tggaaggtga taaggaaatc cagtcagaaa gcgttggtag
    19501 tcattcagtg acttattcaa ctagcaataa aacgatagag caaaaagcat atgatatttt
    19561 aaaaatgtat ttagattcag atttgctgta tcgaggtgtt tagcatgttt tttaaagata
    19621 atataacttt atataatgct tgttataata aagaatttgg agtaaatgaa tattttaaaa
    19681 cttatttagt gggagttgac tggcaaggac aacagaatat aaatgtaaca gataaaggga
    19741 taataagtgc tgatagcgtt aaaataatta tccctttttt agttgcctca aagaataaag
    19801 aatatattac gcctaaagag tttgaaaggc ttgataatgc tgaaaaggaa aagtatttta
    19861 catttaataa taatgataag attgtaaaag gtcttattga ttttgatata gatagcaaaa
    19921 atggaaaaac aataaaaata cttgaaaatc tgtatagtga tgtcataaat ataaactcta
    19981 taattacaca tgattttgga agtttaagta tgagaagttg ggagataggg gggatttaat
    20041 gagtaacaat aatagctctg aaataaagat aaacattgat aatcctgata aaattctctt
    20101 aaaaaggcat ctaaataaaa atggagatgc gcaaaagtta tttacaaatg aaataagaag
    20161 aacaacagac ccatacgtgc cattcaatac aggaatgttg aaaaataatg taattacaaa
    20221 ggttgatgaa atagtatata agtctccaca cgctaaaaaa caatactata aaaataaagg
    20281 atttggaaag caaggaatag cacgtggagg tttgaggggt aagcagtgga taccccgctc
    20341 ctgggctgat aatgggaaga atgttataaa aacaattgct agatttgtag ggggtgaggt
    20401 taaatgacaa taatagaaag tgttagaaat tttataagaa aatgcccaca cttagatgaa
    20461 tttgcaaaag gtataaatat agagttttta aatgataatg taacctctta ttctatagaa
    20521 acagtcccgg cagattctat tttaaaaaag tttgtaaatg gtgattgcat aaaacaattt
    20581 gtatttatct ttgcaagcat agaaagttat ggaaatgaca tactccaaaa cattgaaaat
    20641 agtgactttt acgaaaaatt tgctaaatgg atagaaacgc aaaataatct tggggagttg
    20701 cccgaacttg atggattaaa agagagttta aaaatagaag taactacgcc aagctatcca
    20761 tttcaaacag atatagacaa agcacaatat cagatacagc taagattaaa atattttgaa
    20821 aaaggagaaa ttaattaatg gcaggagtta aacaaagaag aagtatagct aattacttaa
    20881 aaatcgaaga aacattttat ttaatgggtg ctgggtttac agagttaaat gaatccccga
    20941 gtgcgcagac gactagcaaa agatatgtaa atgataagtc agcaacgaaa ggaatttcgg
    21001 gctatgactg gcaaacagca tttacaacag acataattgc aagtgaaaag gctattgaac
    21061 atatttgtaa tattggtaag tatcagctaa caggttcaga cgcagaaaca gaatacatta
    21121 ttgtagattt agacgaggct ttgagtgttt cggaagggga ttacagagca aggaaattca
    21181 atatagcgat agaagtagca gacttttcag atgaagatgg ggaaatgaca gcatccggga
    21241 atttgttagg aataggtgac ccggtagcag gtaaatttaa tacaaaaact aaagaatttg
    21301 tagagggtaa ttttgaatat cctcctaaaa ttcaagaatt aaatattaca agcgcagaag
    21361 gtgaaaatat aggggataca aagataacag taactccagt gattacagaa ggtaatagtt
    21421 ataagtataa attaggtcaa aatctagcat tgccaggatt tgaggaagtt tgtggagctg
    21481 aatatattat ctgggatgga actagcgata ttacagcaac taaagacgat aaaattttaa
    21541 ttgtagaagt taattcaaaa aatagagtct taaaagcagg aattgcaaca gttacagtta
    21601 aggaggaatt gtaaatgtca ataaaatttg aaagaaaaaa tagtataatg gacatggaaa
    21661 tactagggga aaaattcgag gtggattttt cgagggatga aatagctata gtattagcag
    21721 aggtatcaga taatatgact aatatagaaa aagggcttga tatagaagaa ggaactataa
    21781 aacgagcaac aacagaaata ttagaaaaat taaaagaaga agcaagaaga ggaataaata
    21841 taatcttaaa tgatgataca gcatgcgaaa ggatgtttaa agataacaac agcatggcac
    21901 tccatgaaga ggtttacgac tttttagtaa cacaatattc taagtataaa gaaaagcgat
    21961 ttgagaagta tacacctaac agagcgcaaa gaagaaagta aaatatgaac tcaaatatat
    22021 taattgatgt attgccagag cacattaaaa ttgatgataa agtatataaa attaattcag
    22081 attttagaac ttcaatgata ttcgaaatga tgattaatga tgatgaatta agtaatgaag
    22141 aaaaaacgaa tattgcatta aatttatatt atcctagaat acctcatgat aggaatttag
    22201 cgattgaagc aataatttgg ttttatggat gtggtaaaga acgaaaagtt aatggtgaaa
    22261 attctagggc tagaaattca agcaagaaaa acaatagaat ttatgactat gaattagatt
    22321 ctgaatatat ttacagtgca tttttagacc aatacggaat tgatttgcaa gatattaaat
    22381 gcttacattg gtggaagttt agagcgttat ttaaatcttt aaaagaagat aatcagatag
    22441 taaaaatcat aggttatagg tctatagata taacaaaaga tatgtcaaaa gaacaaaaag
    22501 acttctataa aaaaatgaaa aaaatgtatg agatacctaa aagtcaaaca gaaaaagaaa
    22561 agataaatga gatagaaaaa gcgcttttga gtgatggaga tttaagcgat atattgtaaa
    22621 aacaggactc tacaagtata atatatataa aataagtata tgtattatac ttgaaggggg
    22681 gtaattttca tggggtttaa tttcaaaaaa agtataaatt taggttgcgg attaaagttg
    22741 aatatcggca aaaagagtgt cggtataagt gctgggacga aaggtgctcg agtatcagtc
    22801 aacagcaaag ggagaaagtc ggcaacagta ggaatacctg gaacgggtat atcttataca
    22861 aaaactagta ctaaaaaacg taagcctaaa tgattttcat ataaaaacaa tttaatatat
    22921 agataaaaag caattacctt tttaggtagt tgctttttta ttttactccg agggggtgag
    22981 gatttggcag atgggtctat tatacttaag accttattag atacagcagg gatagaaaaa
    23041 ggacttggtg gactcaatgg tgtagcgaaa aagggggcga agctagcaat agcaggagtt
    23101 gctggaatag gtacagcact aggagcaaca acagttaaag cagttaaatt tacagatgaa
    23161 tatactaagg caatgaatgg ctttgaagca tctacaggag aagcaaatgc aaaagtaaat
    23221 ggatttggcg aagccatgca aaatgtatat aaacataact tcggtgaaaa ttttgaggac
    23281 ataggaaacg ctatgtctag cattaagcaa aatttagatg gcattgatgc gtctaatata
    23341 gagaaggtaa cagaaagcgc aatagccatg cgtgatacat ttgatataga tataaacgaa
    23401 ggcttgcttg gagtcaattc tatgatgaaa caatttggaa tgacagcaga ggaaagctat
    23461 aatttaatgg cacaaggtgc tcaaaatgga ctcaatcaaa atggggactt agctgaccag
    23521 ttggcagagt attcggtata ctatagtgat ttaggaatat cggcggaaga aatgttcaac
    23581 atgatggcta atggagcaga aaatggagta tatcagctag attatttaaa tgatgcagtc
    23641 aaagaatttg gaatacgaac taaagacggt tcagctggta caaagcaagc ttttgcagat
    23701 ttagggctta atgcagatga attaacagct aagtttgcaa aaggtggcaa agatgcaaaa
    23761 gaggctttta tggaagttac aacagcgctt ggaaatgtcg aagatgatgt taagagaaac
    23821 caacttggtg tccagctttt tgggacaaaa tgggaagatt tgggcgagga cgcagttaag
    23881 gcacttactg atatggatgg gaaaatatct ggaagcgttg acgcactaga taagataaat
    23941 gagataaaat ataatagttt tggcgaagcc atagaaggca taaaaagaca actagaagtt
    24001 ggcgtattat tacctcttgg tgaagctgtc ttacctcttt taaacgaatt tgcgaactgg
    24061 ttcgctaagg agggagttcc tcagatagaa aagttttcag catccataaa agataagata
    24121 ccagcaatag caacaacttt taaggttgca tttaaagtgc tatcagtttt attgacggct
    24181 tttgtaagaa atattaatat aattattcca gcgctcggct tacttggtgg ggcttttgca
    24241 gtgtttaaag cagttgataa agttcagaaa atggtcgaag catttaagca atttaaagct
    24301 ataacgcaag ctgtaagtgt agcacaaaaa gctttagcgc tagttatgtc aatgaaccct
    24361 tttactatag ccttggttgc gattggatta ttagtagctg gacttgtaca tctttggaat
    24421 acaaatgaag gctttagaaa tgcagttata acagcgtgga ataatattaa agaagttgct
    24481 atgactgtat tcggtggtat atgtaagttt tttactgaaa ttattccaaa cgcatttacc
    24541 tctttcgttg gattcttggg gagtgctttc tcgggtcttg cagatatagc tgggaatata
    24601 atggcaggct taagagatgg atttgtaaat ggctggaact ctattgtagc tttttttact
    24661 gaaactattc caacactcat agggtctatt gttggatggt tcgctgaact tcctggcaag
    24721 atttgggaag gtctttcggc tgtagtaact aagattgggg aatggggtac taatttaaaa
    24781 aataaagctg ttgaagttgg aacaaacttt gttaatacta tagtaaaatt ttttactgaa
    24841 cttccaggga aaatttggaa tgggcttgta atagtagtaa ctaaaattag cgaatgggga
    24901 actactatta aaaataaggc tgtcgaaatt ggaacaaact ttttaaataa tatagttaag
    24961 tttttttctc aacttcctgc aaaaatttgg aattgtttaa aacaagttgt aacaaaaatt
    25021 gctagctggg gaagtagctt agcaagttca gcaaaaaagt cggcaacatc tttagtaaat
    25081 gcttttatta atacagttaa gaccctccca ggaaaattta taagcattgg gaaaaacata
    25141 gttactggac tatggaaagg aatatcgggc aatataaaat ggctaacggg gaaagttggt
    25201 ggatttgcaa atagtatttt aaagaaagct aaatcagtgc ttggaataca ttcgccgtct
    25261 agggttttta gggacatcat aggggctaat atagttaaag gtgttggcgt tggtatagac
    25321 gtagaaactc caagtcttga gaaagatatg gataataatt tatctagttt ggcaaatagg
    25381 ttaaaagcta cagtcgattt agagtctaca aaagcaacgc aaagtattgc agttgcaaca
    25441 aattatacag cagaaaaaga gacacaaaag aaaaataata atgtaaatga agatacaaat
    25501 agtatcttag gaggttttaa tttaaatata gaaaacttca ataataacag agagcaggac
    25561 atggacgact taatgcgaaa agcatcttta tatttaaagc gccatggtat cttagtgtag
    25621 ggggcgttta aattgttttt tatatataaa aatagaaata gcaaagaatt tggaataaag
    25681 attaaaaaaa taaatgattt aagcacgcct aagaggtcta tagaaaaaaa gagtgttcca
    25741 ggcagaaacg gcgaccttgt aattgatgtt ggaaattatg aaaattttac actatctata
    25801 gaatgtgact tagattcaag agatattgat atagaaaatt tagtaacaga gctgattgat
    25861 tggttgcaaa aagactttac atatgaaaag ttatatatag aaaatagaaa ttattattat
    25921 gaagcttatt gttccaatgc ccttgaaata gaacgaatgt acaaaaattt tggcgaattt
    25981 gttatagagt tcgattgtaa gccatttaag cgtatagtta attatagtga tatatcaata
    26041 gacactaaag atatagataa attttctgtt ttcaatagtt acgatatatc atatccagtt
    26101 attgagattt ttggaaatgg agatataagc ttaaaaataa acgaacagga tttaatttta
    26161 aaaggtgtaa aagatagaat tataattgat agtgatgtga tgaatgctta taaaattgat
    26221 gcagtaacta aaaagctggt gaatgaaaac agtaaaatgt ttagtgaatt tccaacccta
    26281 acacatggcg aaaataaaat aacctgggaa ggtgaggtaa atgttattga aataaaacct
    26341 aattgggtaa tgctgtaagg aatttaagta tttagaaagg ggattaaatg agtaaactag
    26401 ataacataaa aggtaatcta aaattgtatg atggaaatga aatagatttc gggcataatg
    26461 gactaggtat attgaaagat gctacagatg cacaagtaac agaagaaata aacggaatat
    26521 ttgagttaga gtttacttat tatgtagact cttttttatt tgaaaaatta aactatggaa
    26581 tgatagtcaa ggctgatgct tcgccaggtc ttagaggtca gttatttaga atttattata
    26641 tatctaaaga gttgatggga actattaaag taaaagcgca acatatatca tacgacttaa
    26701 gagaaaattt tattgaaaaa gtagagtttg aaaaatgtac ctgtgaacaa gctttaaatg
    26761 ccctttttag gggtagtaca gaacaaaatg gttttgttgg acattcaaat ttaacaggag
    26821 aatatagctt tagcatagag tgcgtaaaca catatgaagc tattcaaggt acacgaggga
    26881 gcgttctgga tacttttgga aatggcgcaa aaattaaaag agataacttt aatatatata
    26941 taaatgactc gataggagaa agcaacaatg tattaatagc atataaaaag aatttagttg
    27001 gattggaagc tgaatatgac atccaggatg tagtaactat gatataccct tatgcccagg
    27061 tcgaccaaca atcagacgat actaccgaaa gtgttaaaat tacgcttccc gaaaaatata
    27121 tcgaaagtga aaacataaga aaatacccaa caaggaaaat tgcatatgtt gattttactg
    27181 gagaagatgt aacaaatgta aatcagctaa ggaacaaggc tcaaaaatgg ttttataata
    27241 aaaaagactt accgagtgtc aattacaagg ttgattttat agatttatct caaactgaaa
    27301 attacaaaga ttatgagata ttagaaacag tcaacatgga tgatgaggta attgttagag
    27361 atttgaggat gcaaataaat gcaactgcca gggtcgtaaa aactaattat gaccctatta
    27421 tgaaaagata caattctata gaaattggaa atctggtaaa tcacttcgca ggcttcaagg
    27481 atagactaaa tagtatagaa aatacactag gaaaagtaca cgacgacctg gatgatttcg
    27541 aggtcgataa tgggaagttc ccggacacac ttcctcaaac atcaacgcta aaagcaaatg
    27601 gtttatttgc aacggttcaa ctagagtgga gttatgaaaa taaaaactac tatacttatg
    27661 aattgtacgc ttctcagcta aaagactttg tacccgatac aattactttt aataatagaa
    27721 tatattcggg tcaagcaagc gcctttttac atgaagtaaa gccagttcaa acctggtact
    27781 atagagttag agcaggaaat actcatggga gatttacaga gttttccgac caggtcggag
    27841 catctacaag aaaaattgca gatggagcag agtattttga agaagtggct atcggtcacg
    27901 cattaataag agacttagac gtagataagg ttgtagctgg aaggcttaaa ggtacttata
    27961 tagatgcaaa aaatctatct gttacagatg gaaacggaga tataactttt ttagtagata
    28021 gctttgggaa tgtatctata aaagcaacta acttcacatt aaaaggcaag acactagacg
    28081 atattatagg aactgaaata gatgcaatta cacagcttga aatatttaac aagctaacta
    28141 acaatgggca agcgaaaggt atatatctag tcgggaatga actatttata aatgcttcat
    28201 atattaaatc gggaacgatt gaagggacgt atataaatgc taaaaatcta actgttagag
    28261 ataatagtgg aaatgttact tttgcagtag attcaaatgg aaatgtaact ataagagcag
    28321 tctcttttag cttacagggt caaacaatcg aggaaattgc tacagatata tctactccaa
    28381 tcgcagaaag ggaagcagaa agcgcagtag aagctcaaac acaaagagat atatttaata
    28441 agttgaccaa taatggacag acacaaggaa tttatttaga aaatggaaga atatatataa
    28501 atgcttcata tatccagtcg gggacaatag cgtgcgatag gataacttca agttcaagca
    28561 atccatttat attacttttt gaaggaaatg gcgcaaaatg tgcaatagat gcttcagcac
    28621 agtatggaga aggaataggg aaagcaataa ggcttaaata taacgataat gcttatttgt
    28681 atgtttcaaa tgatgccctt agggggtatt taaacggagt tgaaatattt gaatttggag
    28741 agagaaacgg caaaagttat attcacacag ggctaaatac acaacatata aatccgcagg
    28801 aagcaggaac atataactgt ggttctaata acaaggcatg ggactattta gtatgtaata
    28861 atttaaatca attatcaaga acaacaagtt caaatacgcc atacaggatg gtaaatacta
    28921 gagataacag tagcatagtg gaaaattgta ttgactttgt aagaaatagt gtggtacatc
    28981 cgaatatttt tacaccttac agattaaaaa ctataaatga aaataatata gagcatagat
    29041 tacaagtaga tatagagtca aatttagaaa atccaataag caggtatata tttaaagatg
    29101 tatcagaaga agctggggaa ggggtgtacg cacaagatat aacttcacac ctggcagtac
    29161 ttcaattatc actacaaaag actttaaacg attttgaaaa ctataaaaac gaaactaata
    29221 atactataaa agagcttgca aatagaatag aagcactaga aattagtacg gttaataaga
    29281 attaggaggg gctaaagtgg aaaaatgcat tgaagaaaaa gaacctctag cgaataaaga
    29341 tatattaaat gacatcatta atgtagttaa aaatttagaa gatgaaataa aaaaaattga
    29401 tgatgcaata cttaaaattc caccaactag cgatttaaaa ggggaaaaag gtgagaaggg
    29461 tgagaagggt gaccctggcg aggatttaaa tataaaaggc tcttttgact ctatagatga
    29521 actaccagca gatgcaactc caggtgatgg atatataata gaagggaatt tatacgtttg
    29581 gagcaataat atttgggtca atactgggaa tatgaaagga gagcagggcg acaaaggcga
    29641 taggggaata aaaggagata aaggcgataa aggtgataag gggcaaaata taaaaataaa
    29701 aggtgtctta aactctattg atgaattacc aacaagcgca aatttaggag atgtatatgt
    29761 aatagataga gtaatatata tatttaatga aaaatggctt gaagctggaa atgtaaaagg
    29821 agaaaaaggc aaccctggag acagaggcga aaaaggggag aaaggggaaa aaggaaaccc
    29881 tggagaacct ggttcagatg gagcgaaggg ggggaaggga gaccctggag agcctggtac
    29941 ggatggaacg aaggggggga agggagaccc tggagagcct ggcatagatg gaacttttga
    30001 cgagtcaaag agatttagta cacttataac agcaaataaa acaattataa atgcaactaa
    30061 tgaaataaaa agaaatgcag gcgaagcaaa tcaattttta aaaaatggca agggaatgct
    30121 ggctaacgca ttaacagata aaaatgtccc aaccctttca acagattcct ttgaggttat
    30181 tgctgaaaat ataggaaaaa taaaagagac tcccgacatc cctaaggatt ccgagggggt
    30241 tgttataaga aaagatagtt cagatataat acacaaaata ataaaattag aaacaaatgt
    30301 aacagaagga gaagcaacac caaacgagtt gtggacaaat acaaacaacc ctaattggat
    30361 tttttatatt aatgtagaca atgaagataa tataatttac acgacaggca atatggtaaa
    30421 aaaaataaca acagaaaatg tattggtttg ggaaacgaat gaactgggag caagtaattt
    30481 aagcacagta tgtataaata cagaggggaa aattttagtt gcaagttcaa atggatatat
    30541 aggagtattt aataacgaag gaacaatgtt aaaacgaata gcaggaaata caagacaaat
    30601 acttacagtt aacactgatt ctgaaaataa cgttatatat gcaacgtttg aaaaaaatat
    30661 aaaaaaaatt acttatgagg aagctttaat ttggactttt acagggcata ccggatacat
    30721 aagaggcatt gacatagata ataaaaataa tattataagt ggaagttcag ataaaacaat
    30781 aagaaaaata acgaatactg gagaagaagt ttggaaatac agtggaagca caggcgaggt
    30841 gtcttttgta aaaatagact atcaaggaaa tattgttaat ggcaattcag atgcaacagg
    30901 caaaattacg cctgacggca ttgaaatatg gcaatataaa gatgcgtggc gaggcacttc
    30961 tattttggat gttgactatg aaaataatgt ttatattggt ggcgcagatg gactgctaag
    31021 taagttaaca cctagcggaa ctttaacatg gaaaattacc tggggaaatg gaatatatgc
    31081 aatttcttta gactcaaaag aaaatataat agttggagat agtggaaaca taataaaaaa
    31141 aatgaaaaat actcatacgg ttgagaatat agcttatttt gaataaaaaa agaaggtgat
    31201 atattgagag atacaattta tatagttgat ataaacaata gcttatatca agttgcgact
    31261 tttaaacagt ttgataatga tatagattac aaaataagac ttacagaaaa tggcgtagat
    31321 tctgatttag caggatatac agttaaagca ttttttatgg attctaaaaa aagagtcttt
    31381 caaagaaatt gtactattga agggtctata ataagtacga aattagataa caatatacta
    31441 tcttgcgcag gaattacaac atcagaattt gttttatata aaaatgatat ggcagtagca
    31501 acctacccaa ttacattgaa cgtagtagct ggaatagata gagacgaagc tataaaaaac
    31561 ataccagact gggagattat aaaagatata gttggaataa aagatattat aaacggaaaa
    31621 ttgaaagaac ttgaaaaagc tattgaaaac ataccaacta aggatgaatt agtaggagaa
    31681 aaaggagaca agggcgacaa aggcgatata ggaaaaggac ttaatataaa agggtctttt
    31741 aattcggctg atgaactacc aatggatgca gttttaggtg atgcttatat ggttcaaggt
    31801 aacttgtatg tccacaatgg tacaaattgg attgacgctg gaaatataaa gggagataaa
    31861 ggagaaacag gagacaaagg cgataaggga gataagggcg atactggaga catggggcaa
    31921 ggaattaaaa taaaaggtgt tttaaactct attgatgaac taccagtaga cgcaatgtta
    31981 ggtgatgctt atataataga aaacttttta tatatcaaag gcgacgaatg ggtcaacgct
    32041 ggaaatacta aaggagacaa gggagaacct ggactgaagg gagataaagg agaaaaaggg
    32101 gataagggag aacccggtac tccaggtcag ccaggagcaa agggggataa gggagaacct
    32161 ggcattccag gtcaacctgg agcaaaggga gataagggag acccaggaac acctgggaga
    32221 gatggaactt ttgacgaaac aatggaattt tcttcacttg aaacaactag caagacgcta
    32281 ataccagcta taaatgaagt aaaaacggaa attgacttag taaagcaatc tgccagtagt
    32341 ggaaaagaat tggtcgctac agctattact ggcaaaaata tagctacaga aagtagtgac
    32401 acattcgagg tcatggctgg gaatataaga aaaatcaaag aaatgccagc tatacccgag
    32461 gattcagaag ggattgttat aagaaaagat agtcctagcg tgattcataa aataactaaa
    32521 attgaaatag taacaaaaga tgagtcaagc gaacatatag ttgaaaaaaa agcatactat
    32581 atttaaaaaa ataaggagga attaaaatgc aaaaatattt aacaggatta gaacataaag
    32641 aaggagatat atataaagtt aatttagtac attatatgcc ctttgataca aaaaacggac
    32701 taggaaaaac agtagaagaa ttagaattaa atggtgtctt agttgatgaa ataacagagg
    32761 tagagcagaa agaaggtttt tcaactacaa tgtatgtaaa taaaaaaaca aaagaaatta
    32821 cttacgaata tgtagaaata ccgctaacaa cagaacaaca gatattaaaa tctttaaaga
    32881 atttagagca agataatgca aatataaact acagtttaat gatgggagga ctaatataat
    32941 gctaactttt gataaaataa aatattacta tgagaatcaa atgtggaatg aggaaatggt
    33001 taaaaatgca gtacttaaag aaaaaataac agtaaaagaa tataaagaaa ttacaggaga
    33061 ggactacaaa gcatagtctt tttttattgc actttttaag aaaattaatt aaaaaaataa
    33121 tttaaaaata aaaagctaag aaggagaaaa aaatggaaga actaataaca caattaagtg
    33181 gtttgggagc tgtagggata ttatgtgctt tactatttaa aaacaccatg caggagaaaa
    33241 aagaagatag agatatgtat aaaaagacag tagaaaattt catagaacta tctacacagc
    33301 aacaagagat aaataagaat atacttgttg aaatgggagc aatgaaaaca gacgtagaag
    33361 aaatcaaaga agatgtaaca gacataaaag acatgttaca gaaagaaggt ctttaaatga
    33421 aagtagcaat agtgccaggg catacactaa caggaaaagg aacaggagca acagggtata
    33481 taaatgaagg aacagaaaac aggatattga tggatttggt tgtcaaatgg cttaaaaagg
    33541 gtggagctac tgtatatagt ggtaaggtag ataagtctaa taactactta gcagagcaat
    33601 gtcaaatagc caataagcaa aatgtagatg tagctgtaca aatacatttt aacgctgacc
    33661 atacaacttt ggataaaatg ggtacagaaa caatttataa aactaataat gggaaaaaat
    33721 atgctgacag agttaataag aagttagcga cagtatttaa aaacagaggt gcaaaatcag
    33781 atgtcagagg gctttactgg cttagtcata caaaagctcc agcgatatta atagaagtgt
    33841 gtttcgtaga tagtaaagca gatacagact attatattag acataaagac atagtcgcta
    33901 agttaatagc agaaggtatt ttaaataaga caatagataa taaagaaaat ggcgagggaa
    33961 aaattatgta taaacataca atcgtttatg atggagaagt tgacaaaatc cctgcaactg
    34021 tagttggttg gggctataat gatgggaaaa tattaatatg tgacataaaa gattatgtac
    34081 caggtcagtc agagaattta tatgtcgttg gtggtggagc atgtgaaaag ataggttcta
    34141 ttactaaaga aaaatataca atgataaaag gtaatgatag atttgataca ctttacaaag
    34201 cattagattt tattgataga tagattaaaa ggtagtaact atctctagtt actacctctt
    34261 ttcatttttt taaaattagt aaataaatat cttacttgct gtattctctt tcaagagaat
    34321 ctatattaaa aacccatgac tttccgaaaa gtctacagtc aacaccctca acaaatttac
    34381 catttttaat gttctgcttc aaggtacttt cagccttatt gtacttttta gatgcgtcgc
    34441 ttaaagaaat aaaattacta aaattattta gttgctctat atatccatta taaactccca
    34501 cgacaaaatt aactataacc tcgtaattat ctaagttttc aataatttca gcaggcatag
    34561 gtatgtctaa cttcataaaa gcctttaaaa ttatttcgaa tgctttgttt ctatcttggt
    34621 atctgtaaat tgcatcattt aattctaaaa ttttatttct acttaataca ttctcacttc
    34681 cgattttaaa acctaaatta tatagctctt tatttaattt tgcattcata aaattcactc
    34741 cttgttttgt ttatataatt attatagtcg atacaaacaa aaaaagcaat aaaaatagtc
    34801 ttaaaaaata tttatagtct ttttagacta ttttttattg acaaaatagt cttttaagac
    34861 tataatataa ttaaagataa acaaaagggg gatttaaaaa aatgaaaaag ataactaaaa
    34921 aagaaataat agaatttgta agagatgtag tagaagaata ccgtgattgg aaattagaaa
    34981 aatgtggctt ctatataaag gataatgaat taaattcttt tgtaagcttt gaaggaaaag
    35041 gaattgacat aaatgtatat aaagaaaact acgacgaaat aatttacata gaagattata
    35101 taaaagacta caagcgtaag gaatataact taaaagaaat agattctatc atatacgaag
    35161 atgtaaatga aatgattaat aactataatg aaaaataaaa aaatgaaaat aaaataaaaa
    35221 ttactggagg aaattaaaat gaataaacaa aaagctagaa gatttttaag agttatagat
    35281 atgaatatgg ataaaataga agaagaagct ataaaagctt ttaaagaaag ttgtttaatc
    35341 aaagaggaaa atgatataaa aatttttatt gattttcaag gaaaagttga agcgatagca
    35401 atccaggctt atgctaaact tttagatgat aataaagaaa ttaatatttt cacatcaaga
    35461 caaatagaaa ataacttaga cgatatgttt ggagaaattt gttacgtaaa cgattatgaa
    35521 gaatttgtaa attggtgcga agataaatgc gaaaacttga attgggatag ttataaaaaa
    35581 ttcaataaag aaaatttcga agaaattgct gaaagaaata tagacgatag cacaccagtt
    35641 tttttagaag aattacgaga aggcattgaa agttgtaaac aagaattgca aaatatagtt
    35701 gaaaattaag tagaagagta ttattattaa tgtatatagt atcgctttta agattaacta
    35761 cgagggattt aaaatatttt ttaatttatt tagaaatgat attataataa gaattttcag
    35821 attaactaca agggatttaa atgtcaattt aatttataga gaaatgatat aatatcacat
    35881 tattttacat taactatgag gaaataaaaa cagtgcatcc aataaatgca ctgtttttta
    35941 atgtttagtt attacgataa cttaatcctt caagtataaa ccctacattt tccgtcattt
    36001 caaattcgct actttgcaat attccttccg taaatttgtt gtcatttggt aatgttattt
    36061 ttttccttgc cactgcttct atgattgttt ggaatagttt aattttcata tccttatcaa
    36121 ctccggcgta tcgcaaaaaa tttacaactt cttgtgaaaa cttatttaat tcagatttag
    36181 tcattttttc tgataaatct tttccaattt gcagataaac attcatcttt tccaactcct
    36241 ttttagatta tagttaaaac atattattta aacaatatca atatattgta ataataatat
    36301 taaaatatta cttaaatatt atatatacaa tatttaagta atatctaaac aatataaata
    36361 taatattatt tactttttct tagtcttatt tcttctattt ctctcattgt atatatttaa
    36421 attctcttct tttatagtga catcagataa aatttgagtt agtatatcgc ttaataattt
    36481 gcttacgtcg ctattcatat caatagaaaa agattttaat ttgtttacaa gttcttcatc
    36541 aagcgttgca ctcatagtta cctttttact tttcttatta actagattat atatgtttaa
    36601 tgtcttattg tctgtagttg tagaattatt ttgtatgatt gttccatttt ttttgaaaac
    36661 tggtttttct ttattagata aaagttcttc gtcgaatttt ggcatctata acactccttt
    36721 cacttttagt tcttctagca tattagtaaa ctcctgtctg ctgaaatggt ctttagaatc
    36781 ttttaaaaag tctgttaaag ataatttttt cattagagcc ttttctatga actcgttttt
    36841 tcttatgtat gtctttaaca tcatgctcct taattcacta aaaccttcca aataagaatc
    36901 gaatacttca cttacctgtg tttttttctt ttcaaaacca accaatacga aattttttat
    36961 attgtcctct ttatctagta aagctctgtc agcatcccaa agttgtttga ataattccgc
    37021 tccgcgaagg gatgacacat ttttatcttg tataagaaca attatgctat cagctaaaaa
    37081 aagtacattc ttagcgatta ggtcatagtg gggagataaa tcgcaaatga tataatcata
    37141 tttactaaaa gtatcaaaat tctccatgta ccacttagct aaaatttttt ctttatcatc
    37201 tttaaaatta aataaattct cttctagtaa agtcatctga atatcagaag ctattaaatc
    37261 aacattagaa tattttttat taaaagactt aatgataata tcatcagcac tgcaattttt
    37321 caaaatatca agagtagttt tatcgttatg atttacttta taaacatatt gtgttagatt
    37381 tgcttgtaca tctaagtccc ataagagtat ttttttatct ttatcttttg caagttcata
    37441 tgctgtcatt atagacgttg ttgtcttata tattcctcct tttatgttaa agtacgtaag
    37501 tagctttgta ttagccattt tctccacctc attattatat ttataatatt ataataatat
    37561 catagtattg tttttataat attgcaacaa tatattaata ttattaatta aaatatt
//
LOCUS       DAEORZ010000026        36748 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEORZ010000026 DAEORZ010000000
VERSION     DAEORZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.026"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..1046
                     /locus_tag="KRH39_001156"
     CDS             <1..1046
                     /locus_tag="KRH39_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8511075.1"
                     /translation="NKLREQGQIARYTFKDIKTKNKNMVKCVELAKKISKSDLSVLII
                     GESGTGKELLSQSIHNASNRSNQPFIAVNCAAVPDSLLESQLFGYEKGSFTGALREGK
                     KGLFELANNGTIFLDEIGDMPPLLQTKLLRVLQEKQVMPIGSHNVINIDVRVIAATNK
                     NLLKMIREGKFREDLYYRLNVLPIDVPSLRNRKEDIITLMNFFLKNNIRLSPEVITIL
                     EKYNWPGNIRELQNVASYVSLMCDSMVFRDDLPPYIRVECANNYEDGFGYTRKEFEEI
                     LRILYERRESSSGIGRGFIIEKLIQEGIEISESKVRKVLSYLSECGYISSKSGRGGSQ
                     ITSEGIKLYNNIN"
     gene            1397..1642
                     /locus_tag="KRH39_001157"
     CDS             1397..1642
                     /locus_tag="KRH39_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG8511076.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            1677..2402
                     /locus_tag="KRH39_001158"
     CDS             1677..2402
                     /locus_tag="KRH39_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG8511077.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNMDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCNKMEKLFKYFINICSNL"
     gene            2424..3737
                     /locus_tag="KRH39_001159"
     CDS             2424..3737
                     /locus_tag="KRH39_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG8511078.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKAFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYGENLKKIV"
     gene            3943..4482
                     /locus_tag="KRH39_001160"
     CDS             3943..4482
                     /locus_tag="KRH39_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8511079.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            4571..6556
                     /locus_tag="KRH39_001161"
     CDS             4571..6556
                     /locus_tag="KRH39_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBG8511080.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            6780..7748
                     /gene="spoIIIAA"
                     /locus_tag="KRH39_001162"
     CDS             6780..7748
                     /gene="spoIIIAA"
                     /locus_tag="KRH39_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBG8511081.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIDD
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            7738..8259
                     /locus_tag="KRH39_001163"
     CDS             7738..8259
                     /locus_tag="KRH39_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBG8511082.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            8276..8473
                     /gene="spoIIIAC"
                     /locus_tag="KRH39_001164"
     CDS             8276..8473
                     /gene="spoIIIAC"
                     /locus_tag="KRH39_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBG8511083.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            8489..8875
                     /gene="spoIIIAD"
                     /locus_tag="KRH39_001165"
     CDS             8489..8875
                     /gene="spoIIIAD"
                     /locus_tag="KRH39_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBG8511084.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            8960..10165
                     /gene="spoIIIAE"
                     /locus_tag="KRH39_001166"
     CDS             8960..10165
                     /gene="spoIIIAE"
                     /locus_tag="KRH39_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBG8511085.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMISLTLVGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            10225..10842
                     /locus_tag="KRH39_001167"
     CDS             10225..10842
                     /locus_tag="KRH39_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBG8511086.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGSYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNSSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            10854..11408
                     /locus_tag="KRH39_001168"
     CDS             10854..11408
                     /locus_tag="KRH39_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBG8511087.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            11435..12124
                     /locus_tag="KRH39_001169"
     CDS             11435..12124
                     /locus_tag="KRH39_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBG8511088.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDGDSSKTEDSSNVDKQDSKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKT
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            12234..12596
                     /locus_tag="KRH39_001170"
     CDS             12234..12596
                     /locus_tag="KRH39_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG8511089.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            12681..13187
                     /gene="nusB"
                     /locus_tag="KRH39_001171"
     CDS             12681..13187
                     /gene="nusB"
                     /locus_tag="KRH39_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBG8511090.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            13171..14241
                     /locus_tag="KRH39_001172"
     CDS             13171..14241
                     /locus_tag="KRH39_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBG8511091.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNRFISVHMSGGTTEILLTIKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            14238..15443
                     /locus_tag="KRH39_001173"
     CDS             14238..15443
                     /locus_tag="KRH39_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBG8511092.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            15445..15660
                     /gene="xseB"
                     /locus_tag="KRH39_001174"
     CDS             15445..15660
                     /gene="xseB"
                     /locus_tag="KRH39_001174"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBG8511093.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            15663..16550
                     /locus_tag="KRH39_001175"
     CDS             15663..16550
                     /locus_tag="KRH39_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBG8511094.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLNAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANFEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            16580..17017
                     /locus_tag="KRH39_001176"
     CDS             16580..17017
                     /locus_tag="KRH39_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBG8511095.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            17051..18916
                     /locus_tag="KRH39_001177"
     CDS             17051..18916
                     /locus_tag="KRH39_001177"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBG8511096.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANNNED
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEDMLKVLLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNADELYDFVGLSPKSIADKIRKLVIE"
     gene            19049..19861
                     /locus_tag="KRH39_001178"
     CDS             19049..19861
                     /locus_tag="KRH39_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBG8511097.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEIVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGEIYNNKIVEVVEASHNLDK"
     gene            19881..21560
                     /gene="recN"
                     /locus_tag="KRH39_001179"
     CDS             19881..21560
                     /gene="recN"
                     /locus_tag="KRH39_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBG8511098.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNIYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGESELEGFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLKSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            21776..22642
                     /locus_tag="KRH39_001180"
     CDS             21776..22642
                     /locus_tag="KRH39_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511099.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDIGNTINEEMSKSYITKSIDLKVKKIEKKECIIDIDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(22735..23226)
                     /locus_tag="KRH39_001181"
     CDS             complement(22735..23226)
                     /locus_tag="KRH39_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8511100.1"
                     /translation="MADKMVQNITLQFVEENDLENLENVKLLFTEYSKSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEYKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKIGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(23287..23727)
                     /locus_tag="KRH39_001182"
     CDS             complement(23287..23727)
                     /locus_tag="KRH39_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8511101.1"
                     /translation="MKLNVRIADINNWRDLVNLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNKY
                     NCDELYLSIFENNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            23888..24946
                     /locus_tag="KRH39_001183"
     CDS             23888..24946
                     /locus_tag="KRH39_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG8511102.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMGIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDIDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKQENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNIIENHNKKFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            25088..25912
                     /gene="spo0A"
                     /locus_tag="KRH39_001184"
     CDS             25088..25912
                     /gene="spo0A"
                     /locus_tag="KRH39_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBG8511103.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            26325..27446
                     /locus_tag="KRH39_001185"
     CDS             26325..27446
                     /locus_tag="KRH39_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511104.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHVDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            27464..28339
                     /locus_tag="KRH39_001186"
     CDS             27464..28339
                     /locus_tag="KRH39_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBG8511105.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIVLKENISNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDNTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            28363..29055
                     /locus_tag="KRH39_001187"
     CDS             28363..29055
                     /locus_tag="KRH39_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG8511106.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            29063..29887
                     /locus_tag="KRH39_001188"
     CDS             29063..29887
                     /locus_tag="KRH39_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG8511107.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            29892..30959
                     /locus_tag="KRH39_001189"
     CDS             29892..30959
                     /locus_tag="KRH39_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBG8511108.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            30961..31497
                     /locus_tag="KRH39_001190"
     CDS             30961..31497
                     /locus_tag="KRH39_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG8511109.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            31565..32170
                     /locus_tag="KRH39_001191"
     CDS             31565..32170
                     /locus_tag="KRH39_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBG8511110.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            32182..33096
                     /locus_tag="KRH39_001192"
     CDS             32182..33096
                     /locus_tag="KRH39_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG8511111.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            33138..34310
                     /locus_tag="KRH39_001193"
     CDS             33138..34310
                     /locus_tag="KRH39_001193"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBG8511112.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            34357..35169
                     /locus_tag="KRH39_001194"
     CDS             34357..35169
                     /locus_tag="KRH39_001194"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBG8511113.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            35373..36698
                     /locus_tag="KRH39_001195"
     CDS             35373..36698
                     /locus_tag="KRH39_001195"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBG8511114.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
BASE COUNT        14871 a         3660 c         6773 g        11444 t
ORIGIN      
        1 ctaataaatt aagagaacaa ggtcagatag caagatatac atttaaagat ataaaaacta
       61 aaaataaaaa tatggtaaag tgtgtagaat tggctaaaaa aatatctaaa tcagaccttt
      121 ctgtattaat aataggagaa agtggtactg gtaaagaatt attatcacaa tcaattcata
      181 atgcttccaa tagaagcaat caaccgttta tagcagttaa ttgtgcagca gttccagata
      241 gtctattaga aagtcaactg tttggatatg aaaaaggctc ttttacagga gctttaagag
      301 aaggaaaaaa gggtctattt gaacttgcta ataatggaac tatatttctg gatgaaattg
      361 gagatatgcc tccattgcta cagacaaagt tactaagggt attacaagaa aagcaggtta
      421 tgcctatagg ttcccataat gttataaaca tagatgttag ggtcatagca gcaacaaaca
      481 aaaacttact aaagatgatt agagaaggaa aatttagaga ggacctgtat tatagactaa
      541 atgtattgcc aatagatgta ccatcattga ggaatagaaa agaagatata ataacactta
      601 tgaatttctt tttgaaaaac aatataagac tttcgccaga agttataact attttagaaa
      661 aatataattg gccaggaaat ataagagagt tacaaaatgt tgcttcttat gtaagtctaa
      721 tgtgtgatag tatggttttt agagatgact taccaccata tataagggta gaatgtgcta
      781 acaattatga agatggattt ggttatacaa gaaaagaatt tgaggaaata ctgaggattc
      841 tatatgaaag aagagagtca tcctctggaa ttggaagagg gtttataatc gaaaagctta
      901 tacaggaagg tattgagatt tctgaaagca aagttagaaa ggtgctatct tatttaagtg
      961 aatgtggata catatcatct aaatctggta gaggaggaag tcaaataact tcagagggga
     1021 taaaacttta taataatata aattaagcta agtatttgat attgaaatca catattttga
     1081 atttagatat ttaaataata ataaactttt atatttatag agattaattt atattatgtc
     1141 agaaagaata ttgtaattta taattaaata ttaataggtt tttaaaatag gattagatag
     1201 gtaataggtt aagttagaat aacccattac ctatttttat ttgtaagaaa agtgtacata
     1261 gaattagaag taaaaaatac aactaataaa actattgaaa agactagata aataataaat
     1321 aaaaataatt atagattttg gcatacatat tgctaattat agagataaga cgacaaaata
     1381 attgggaggt ttaatgatga aatacaaaac aacagggact tgtgctacag aaattgaatt
     1441 tgaagtaaaa gaaaataaag ttacaaatgt aaactttata ggtggatgtg atggaaattt
     1501 aaaagggcta aaagtattag ttgaaggcat gaatatagaa gatgtaatac aaaagttaaa
     1561 aggaatagag tgtaaaacaa agccaacatc ttgcccagac caactgtctc tagcattgga
     1621 aaattatatg aatataaagt aatacataaa acaatcgatt agttaggagt ttaattatga
     1681 aaccaataat agggatttta ggaaatttaa taataatgga aaatggtatg tttccaggtc
     1741 ttgaaaggtc atatgtaaat aatgattata taaatgcagt gctaaaaggt ggcggtagtc
     1801 cagtaataat tcctgtaaat atggataaag aagttataaa aaaacagatt gaaatggttg
     1861 atggagtctt aatatctggg ggatgggata tcaatcctca actgtatgga gaagaaacaa
     1921 gagaagaaac gacatttata tatccagaag tggatgaatt tgatttaatt gcaataagta
     1981 tagcattaga acttaaaaag cctatattag gtgtatgtag gggactacaa attttaaatg
     2041 taagtttagg aggaacttta tatcaagata ataatcttat agaaggaagt tatataaaac
     2101 atacacaatc ttctaaaaga catgtagcta ctcataaagt tgatgttaaa gaaggaagta
     2161 ttttggaggg aattttagga aaacaattac ttacaaatag ttatcatcat caaagtgtca
     2221 atcagcttgg aaaaggatta aaagccatag catactcaaa agatggtata atagaagcta
     2281 ttgagaaaga ggatgaaaat tttgtagtag gagtacagtg gcatccagaa atgatggtag
     2341 attactgtaa taaaatggaa aaactattta aatattttat aaatatttgt tcaaatttat
     2401 aatatacctt ggggggattg aacatgaacg aaaagaacaa aatgggattg ataagtatta
     2461 tacttttagg tattaatgca gtagtaggag ctggagtttt tctattacca ggagatgcaa
     2521 tgaaagcatt tggtgtagca agtatatttg tatatatatt tgatatgttg ttagttttat
     2581 ctatggcatt ctgttttgct gaagttgcag gtaaattcaa taaaaatggt gctgcttatg
     2641 tatataccaa agaagctttt ggagattttt gtggttttga agtaggtctt atgaaatggg
     2701 taataggatg tatatcttgg ggagctttga tagtaggttt tcctacatca ttatcagcag
     2761 tatgggctcc agcaggtgaa ccacatatac aaaaaattat aattgtagcc atgatagttg
     2821 ggcttacaat tataaactta ttgggtgtat ctctatcaaa aattgtacaa aatgttataa
     2881 ctgtaggtaa actaattcca ttaatactat ttataggaat aggtatattt tttataaaag
     2941 gagttaattt tacttcttca acaatggtac ctccaggagc tggagcaaca gagtttggag
     3001 cagcagcact tttaatgttt tattcattta caggatttga atctatagca gttgcagcag
     3061 aagatatgga aaatccacaa aaaaatatac caatagcaat tataagtgta atagtaattg
     3121 catcaataat atatatatta aatcaagttg tatgtgttgg tatactagga gattcgttat
     3181 ctagtacatc tacaccagtg gcagatgctg caagaatatg ctttggaaat atgggagctg
     3241 gattggttac ctttggaaca ttggtttctg ttggtggaat ttgtatgtgt ggtgcttttg
     3301 taaatccaag aagctgtgta gctttagctg atgataaaat gttaccgaga atatttgcaa
     3361 gaaaagataa aaaaggtaca ccatatgtag cgataatagc tactatgtta atcactatac
     3421 caatagctct atctggaagt tttgctgaat tagcagcaat aagtgcagtt gctagattca
     3481 tacaatacat accaacatct ttatccgtat tagtgtttag aaagaaaaga ccagaattag
     3541 taggtacttt taaaactcct tttggagcag tgattcctct aattgctgta tgtgttggaa
     3601 catggttatt attccaagca tctatgcatc aattaattat gggattagga gctttggcaa
     3661 ttggagtacc attatacttt ataatgaagt cttataatag aaaagtctat ggtgaaaatt
     3721 taaagaaaat agtttaatta tgttataaca gtttattgag ttaaaattag acattggcag
     3781 aaaattaaat acaaataagt atctataagg ctatattaaa atattattaa ttttttattt
     3841 taatatagcc ttatattgtt atcaataaat gaaaaatatt gtaacaacag aagaacaata
     3901 gtattatgtt tatagacaaa aaacttatta ggaggtaaat ttatgccaac aataacatta
     3961 aaaaatggtg tagacgtact tattagagaa ggagtaaggg aagatgctca aagcattata
     4021 gatttttata atgaagtggg tggagaaact cattttctgt catttggaaa ggatgaatat
     4081 aaaatatctt tggaggaaca agaaaatgct atagaatcag caaaagcatc tgataattct
     4141 gttaaactga ttgcatttat agatggtgaa atagttggga ttgcaacgat tgattctaat
     4201 caaaaagcaa aaggaaagca tgttggagta ttaggaattg ttgtaaaaga aaaatattgg
     4261 ggaattggat tgggtaaaag gcttatgtta gaccttatag aatggtgtaa atcaaatgga
     4321 ataaccaaga agataactct tgtcactaat gaagaaaatt ataatgctat aggattatac
     4381 aaaaaagttg gatttgaagt agaaagcata ctaaagaaag aatgttatta taatggtgtt
     4441 tatactgatt taataggaat gtcactttta ttgggaatat aattagtgta cattttatct
     4501 cgaaaaaagt atattaagta tggtataatt aaaaaagaat ataacttaat ataattttgg
     4561 aggacgaatt atgaaaaatc tgtgtaaaat atcagatgat tatttaaaca gcaagcttaa
     4621 gtatttaaag ttattatcaa aacaatatcc aagtatatca aaagcaagta cagaaataat
     4681 aaatttagag gctatattaa atcttccaaa gggaacagag cattttataa cagatgtaca
     4741 tggtgaatat gaaccatttg tacatgtatt aaaaaatggt tctggtgtaa taaagagaaa
     4801 aatagaagaa ttgttttcaa atacaataag agacagcgaa aaaaaaatgt tagcaactct
     4861 tgtatattat cctgaacaaa agttagacct aataattaag caagaggaaa atatagatga
     4921 tttttataga ataaatattt atagacttat agaactttgt aaatatgctt ctagcaaata
     4981 tactaggtct aaagttagaa aactgttacc agaaaatttt aaatacataa tagaagaact
     5041 tttgcatgag catgttaaaa gtgaacataa agaagagtat tataaaagta tagttgaaac
     5101 aatagttgac ataggaatag ctaaggagtt tataattgct atttctacag ttatacaaaa
     5161 attggttgta gatagacttc atgtaatagg agatatttat gatagaggtc caagaccaga
     5221 tataattgtc gataagctta ttgaacatca ttgtgtagat attcaatggg gaaatcatga
     5281 tatattgtgg atgggtgcag cctcaggtga aaaaacttgt atagcaaatg cactgaggat
     5341 atcagctaga tatgcgaatt tagacattgt agaagacata tatggaataa atctgttgcc
     5401 tttggccact tttgctatag aaatgtataa agatgaccct tgtaaagaat ttattccaaa
     5461 ggttaatgac caaagtgtaa caacaacaga aaagtcttta atggcaaaaa tgcacaaagc
     5521 aataagtata attcaattta agcttgaagg tgaggttatc agacgaagac ctgaatttga
     5581 aatggaacat agactcttgc ttaatatgat aaactatgat gaaggtacga ttaatttaaa
     5641 aggtaaaaca tataaattaa aagatactta tttacctaca atagacaaaa aagacccata
     5701 taaattaact atggaagaaa gaaatgttat tgataaatta gtatcttcat ttagaggtag
     5761 tgaaaaatta caaaaacatg tttcattttt gttttctaaa ggaagtatat atttaaaggc
     5821 taactcaaat ttactgattc atggatgtgt accgttaaat gaagatggaa gctttatgtc
     5881 tatgaatata atgggaaaag agtataaagg taaggctctt atggataaga tggagtctct
     5941 agcaagagaa ggatttttct ttaaagataa ggcagaagaa aaactgtatg gcatggatat
     6001 tatgtggtat ttatggactg ggaaatgttc atcattattt ggtaaagatg atatgactac
     6061 atttgaaaga tactttatag ctgaaaaaga gactcataaa gaaaataaga acccatattt
     6121 taaacttaga gaaaatgaga tggcttgtaa aagattattt gaagaatttg atttagaact
     6181 tgatgaatct catataataa atggtcatgt tcctgtggag agtaaaaatg gagagagccc
     6241 aataaaagct aatggtaaaa ttcttgttat agatggtggt ttttctaggg cttaccaaaa
     6301 aactactggt atagctggat acactttgat atacaattct agaactttgc aattagtatc
     6361 gcatgaacca tttaattctg ctgaagaagc cattgcaaat gaaagtgata tcttatcgac
     6421 aactgttgta gtagagcata aggcaaagag aaaaatggtg agagatactg atgaaggaat
     6481 aaagatacaa gaggaaatag aagatttaaa actactttta atggcttata agaaagggct
     6541 aataaaagaa atgtagatgt tatagaggca cttcatgatt gaagtgtctt tttgttaata
     6601 cttattaaat atattataaa gattaataat agtagtttat attatactta ctaagtgaat
     6661 ccaagataaa tgggttcact ttttttattt ataaacatta ttaaaaaaat atagatgttt
     6721 tggttatatt ccttgtcctt tataaataga attatataaa caaaggagga ggacaagaga
     6781 tgaataaact ttctgatgaa ataataaact ctctttctac aactataaga gaaaaggtag
     6841 aaaaagtctc aaacaataat ctgaacatag aagaaattag gttacgctca caaaaaccat
     6901 tgatactaaa tgcaaactct aaagattatt tttacaatca aaaaactatg acacttgatt
     6961 taaatcaaca aaactcatat gtagtaacaa gagaagatgt agagcaaact tttcaaataa
     7021 tatgtaaata ttcaatacat tcatttatgg atgacataaa aaaaggattc ataactttga
     7081 gaggaggtca tagagtaggg ttagttggaa aagcaatagt agaagatgga caagtcaaaa
     7141 atataaagca tatatcatct ttaaatataa gggtatcaag ggaaattatt ggatgctcgg
     7201 ataagatttt aagtcatatc ataaagggaa aaaatcagat taataatact ttgataatat
     7261 caccacctca atgtggaaaa acaactttaa taagagatat agtaagaaat ttaagtaatg
     7321 gtaatgaaga ttatggattt aaaggtttaa aagtggcttt agtagatgag cgtaatgaga
     7381 tagcaggagc ttatctaggt gttccacaaa tggatgtggg aataaggact gatataatag
     7441 aaacttgccc aaaagacttg ggtattacaa tgcttttaag gtctatgtca ccaaatgtta
     7501 ttgtgacaga tgaaattgga agtgaaaaag aaataaaggc attatacaca gcattaaatg
     7561 gaggtatagg attaataact actgtgcatg gagattcaat agatgatata caaaatagaa
     7621 aagaattaaa tagacttttg gataaagagt tatttaaaaa ggttataatt ctttctgcaa
     7681 aaagaggagc aggaacgatt gaaaaaattt atgatttaga agaaaagaga tggtattttg
     7741 caaattaaaa taattattat agctttcttg ataggaagta gttatttgat aggtgaacaa
     7801 atatacaaaa cttatactcg tagacataaa caattaaacg atttaataag agttttagaa
     7861 attttgagga tggatttatc ttttggactt tatacattag aagaaatctt taatagaata
     7921 ggtggaaata aagagttttg tttttggaaa tttttctatc aaatatcaga aggacttcat
     7981 aatgaacaaa gtaaaacttt ggaaataata atatcagaaa atattgatgt attaagtaaa
     8041 gaaacttatc taggcaataa agaaattgag gaactgaaaa atctaatact tactttaggg
     8101 aaaagcgata tagagtcaca acaaagaatg atagatttat ctatagaaaa tttgaaaaaa
     8161 caaacctatg agacaaagga agatataaac aaaaaaggcg tattgtataa aaaattagta
     8221 acattcatag gtataggtat atgtattata ttgatttaat atggggaggt ttattatgga
     8281 aatatcattg atattaaagg ttgcaggtgt tggtattcta atatcagtat tgaatatgat
     8341 attggaaaaa acagatagaa aagattgggc aggtcttact acactagcag gtgtaataat
     8401 cgtattgggt atggtaataa cagaaataag tgacttgttc aatactgtaa gaacaatgtt
     8461 tcaactttac taaagaggag ggagtaattt ggaaataatg caattaatag gaattgcaat
     8521 catttcaact acactctgtt tagtaattaa aaaagataga ccagagatag caaatttcat
     8581 agcaataata actggagtaa taatactatt gtcagttatg tttaagctaa attttatagt
     8641 agatagtata caagatttgg ctaacaaggc taacatcccg actatgtata tatcattaat
     8701 aataaaatta attgggatag catacttaat ggaatttgcg atacaacttt gtaaagattg
     8761 tggggaagga aacattgctt caaaactaga atttggaggt aaaataatag ttatgtcaat
     8821 gtctttccct atacttttgt ccattgtaga aatggtggtg aatataattc catagatgtg
     8881 tatcttggaa tgactataag tatattagag agctttcgtt gtattgaggt ttatagagtg
     8941 aagagaggca tggttgctta tgaaaaaaag atttttacca atgattatag gttttttatt
     9001 tactttcttt tttgtaaata tctttgctat tatgatattt gctaatgaag taccaagtag
     9061 tgaagataaa gaatatggtg aaacaaaaaa tagcatagat aagtatatag atggtcaatt
     9121 agacaagcta gatataaatg agattcaaga ctatataaat aaagaaatag ttattaatga
     9181 cgttaattta aaatcatttg taaaagattt aattagtgga gaaaaaaata tcttagattt
     9241 attcaataaa gatggattga agatattgat gtttgatgaa tttaaagcaa gtttaaaggt
     9301 agtagcagta attttggtac tggctctgtt atcatctatt ttaaagagtc tggaaaactc
     9361 tttttcatca ggtgcagtaa gtcaaatagc aacatatata atatttatca caatgatatc
     9421 acttacactt gttggtttta aagatgtatt acagatttgc tatgatgcaa tagaccatac
     9481 tgtaggtctt atgcaagtta taatgccaat attaataaca tttttacttt taattggatt
     9541 cccaataacg tcaactactt tgaatcctat cttcatagga ggagtaacat tcataaatgt
     9601 attttttaaa aattttctat ttgtatctat tactgttgca tttgggattt taataataaa
     9661 caatttatca aaaaatataa ggctaaagag atttttctcc tttgtaaaac aaataaatta
     9721 tgtttctatt ggagctatgt ttactgttta tttaggtctt gtatccatac aaggattgta
     9781 tgtaaccagt tttgataaat tcagtgtaaa gactgctaaa tttgctatag gtaattttat
     9841 tcctgttgta ggtggatttg tatctgactc tgtggatatt ttattatctt catctcagct
     9901 tataaaaaat atatttggag gaatagggct tatcttactt gttggaattt gtttacttcc
     9961 agtaataaaa attttgtcag taatagtagt atacaaatta gcagcaataa ttgtagagcc
    10021 tgttggagag gatggaatat ctaatttttt aaatgaagtg gcaaatttaa tgataataat
    10081 gttagcatca gtgattgcta taacagtaat gttctttgta actgtagcta tcttgacctc
    10141 gataagtgtc gtaagtcaag ggtaatttat atatcagtga aaaataatta catttaatct
    10201 aatgttttta gagaggagga agctatgtta gagggcataa aggcatggat agttagtgtt
    10261 ttgataggag catttatagt aaatatagta gatatgattt taccaagttc taagataaaa
    10321 ccttatgtca acctagtttt aaactttatg tttgtattta ttgtaataac tccagtagta
    10381 ggattttttt caaaagatat gagtttagaa gatagaatat taaaatctat gggaagttat
    10441 aataaacagt atgtagatag tacaaatgct ttggcaaaag agactggaaa taatagtcta
    10501 tccaagggat atgaggatgg gctaaaagaa gtacttaaat taaaacttga tgaatatggt
    10561 tatgatttag aagatataga gcttaatgga gcaaatataa ataatataaa aataaaagaa
    10621 aagaataata gtgcaaaaag taatagcagt tccaatgaag aaaataaaaa taatagtact
    10681 aaaagagagg aagaaaacat aaactctaat gacaaagaaa attctaaaca agtcttcaaa
    10741 aaaggtacag agtatgggtt gaacctaaat gaagaaaaat taaaaaatga cttaataaaa
    10801 gtgcttgatg tttctataga agatatacaa attgataaat aggagttggg agaatgttga
    10861 aaaacttaaa tgacaaagat aagagaaagg tatattcact aataactata gcaggaatat
    10921 gtgtggtttc attagtatta ctatcttgtt ttcctagtag caaaagcgat aaacaagttg
    10981 gaaaaacaga gactaataaa aatgctgaaa agcaagtcac taaagaacaa gaaaaagatg
    11041 atttagagtc aaaattaaca gcaatactat ctaaaattga tggtgcagga gacgtcgatg
    11101 taatggttac atttgaatca agtgaagaaa tacaacctgc ttttaattca aataatacaa
    11161 cagaaacaac agaggaaaaa gatgcacaag gtggagaaag aacagttacg acttctagtg
    11221 aaaataaaac aatgataact tcaaattcaa gtgaccctgt agttataaaa actacagagc
    11281 caaagataaa gggagtgata gtggtagcaa gtggagcaag tgacccaagt gttaaagaga
    11341 cactttatag tgcagtacaa acatcattac aagtagctgg tcatcaagta gaaatatatt
    11401 ctaaataaat acatataacc ggggggataa atgaatgaag tttaattata agggaagagg
    11461 atttgtaata ataactttaa ctgcaatgtt agtggtagta ggaacagtga attatcaatt
    11521 aagtaagaaa tctttattag aaacatcaaa agagtttaag gcatatgaag aagcacagtt
    11581 acaaaaaaat actgatgatg gtgatagtag taagacagag gattctagta atgtagataa
    11641 acaagatagt aaggaaagtg ctgatattga tatagttgat agcaaagcta gtaaagtaaa
    11701 agaaaaggca actgagacaa gtaaggaaat aaaagctcaa ttatcatcag agaaaaatat
    11761 gaaaaaggca tcttatattt tagatatgaa aatgaataga gaaaaacaaa gaaatgaatt
    11821 agttcaagat ttaaatgaaa tgataaataa tccatctaca acagaagaat ctagaaaaga
    11881 agcttctaat atgaaattaa atatagttaa aactcaagaa aaagaacttc aaatagaaaa
    11941 tcttttgagt acaaaaggat atgaagaagc attagtttat ataagcgata acaaagttaa
    12001 tgtagttgta aacgaagcca aattagaaaa gaaagatgca gcaaaaatat ttgatttagt
    12061 tgcagaacaa gccaatgtaa agtatgaaaa tattaaactt acaaataata atagtaataa
    12121 gtaaagtata aacctcagaa ttaactgagg ttattttctt gcaaagtaga cttgtatttg
    12181 gtattattat acttataaaa atagttcctt aaaatcagga ggtaagtttt aatatggaag
    12241 ataataaatt tggacaagta aaaatatcta atgatgtaat agctacaata gctggtttag
    12301 cagcattaga agttgaaggc atagaaacta cagcaacatt aacagataag ttgttaaaaa
    12361 ataatggagt aaaaatacaa atcgaagaag aggatgttaa tttagatgta atggttacga
    12421 taaagtatgg aatgtcaata ccagatacag cttttaaagt tcaagaaaat gtaaaaaata
    12481 ctgttgagac aatgacagga ctgaaggttt ctcaagtaaa tatacacatt caaggaatca
    12541 gctttaagaa ggataaagtt gataaagaag aagcaaaagc aactaagaaa aattaatatt
    12601 agccctctga aaagtcagag ggttttttgc tggtaattga ttttatatgg ataattaaac
    12661 ttaaaggaga attttgattg atgaaaaaag atagggcaca aaagagtaca acaagagaat
    12721 atataatgaa acttatatac caaataaata ttaataaaga agattttgaa actttagaag
    12781 ataaagtaga taatttttta aaagataatt cagaacatat tataaataga tataaagagc
    12841 ttgcacttca atactcaaaa aatacaaatt taaagttaga agatactgaa attgaagatg
    12901 ttatagacaa aaaatatata aatacagtat gcaaagcatt gaaagaaaat catgacaaaa
    12961 tagatgagtt aataaataag catgctaaaa actggacagt agacagaatg ccaaaggtag
    13021 atgtttctat acttagattg tctgtgtgtg aaatacttta tttagacact cctaataaag
    13081 tatcaataaa tgaagcagta gaacttgcaa aaatatattg tgatgataag tctcctaaat
    13141 ttataaatgg tatattggga agtgttgttg atgaaattgg gaaataatat aataattgga
    13201 attgatacta gctgctatac tacttctata gcagctatct ctttagataa gaaagttata
    13261 ttcaatgaaa aaataatgtt agaagtaagg gataattcaa aaggtttaag gcaaagtgaa
    13321 gctgtattcc aacatatcaa taatttaggg attcttagtg ataggataaa atcttttaaa
    13381 gataagttta atgtagaagg agtttgctca tcaaaaaaac cgagacctgt tgaaaactca
    13441 tatatgcctg tattcaatgt aggtcataat tttggaaaat tattgtctag tatatatggt
    13501 tgtaggtttt atgagactac ccatcaagag aatcacattg aagcaagttt attaaatagt
    13561 aaattaaaaa ataataatag atttatttct gttcatatgt ctggtggtac tacagagata
    13621 cttttgacaa tcaaacaaga tagtcatcac aatgtatgtg atactaattt aggtaaaatt
    13681 gccaaaataa gtattaaaaa agatgataaa agtaagttat acaataattt tggatataat
    13741 atagatataa ttggaggaag taaagatatt agctttggtc aacttataga tagagtagga
    13801 attaagcttg gatataaatt tccttcagga aagtatttgg atgaaaatgc tttaaattgt
    13861 aatcttaaaa tagaaagtgg tctgaaaact tctgtaagag atgggtatat gaatttatct
    13921 gggttagaaa atcaggtaaa taagattata aatgataatg gagataatac taatcaaaaa
    13981 gaatacatat ctaaattagt attagattca gtagttagaa atatgtttaa atctttagta
    14041 tatttatgtg aaacttataa tgtaaatgaa gtagtatttg ctggtggagt ttctgcaagt
    14101 aagtatattc ttagagaatt aagcatgaag ttaagaaaaa agcatataga agcttatttt
    14161 acagaaccac aatattcaac tgataatgct gtcgggtgtg ctataatagg attgaataac
    14221 tttttaggag aaagagtatg aaactaagag ctttagatat aagtgaagca aactcatata
    14281 ttaaaagaat tttaataaac gacccaatac tttctaatct taaagttaaa ggagagattt
    14341 ctaattttaa ggttcatagt agtgggaatg tatatttatc acttaaagat gaaacatcaa
    14401 agttaaactg tgtaattttt aaaagtaatt ttaatcgcaa tttaaaatta gataatggtg
    14461 taaaaattat agctaatgga tatatatcag tctatgaaag agatggtgcg tatcaattat
    14521 acattaatga aattgaaata gagggaatag gaaatttaca catagagttt aatagattaa
    14581 aggaaaaact taataaagaa ggattatttg accctaaata caagatacct atacctaaaa
    14641 tgccaaactc aattggcgtt ataacatctc caacaggtgc tgttataaga gatataataa
    14701 atgtaataaa aagaagatat ccgaaagtta atataaagct ttatccagta acggttcaag
    14761 gagataaatc agctgaagaa atctgtgaag caattagatt tttcaatcat atgaagaatg
    14821 ttgatacact tatagttggt agaggtgggg gttcaataga agaactttgg tcttttaatg
    14881 atgaaatggt agcaagagag gtttttaatt ctcaaattcc gataatatca gcagttgggc
    14941 atgaaacgga ttttactatt tgtgactttg tgtctgatat gagggctcca acgccatcag
    15001 cagcagctga gattgctact ccatcattgg atgatataaa ctataaactt ggaaacataa
    15061 aaagcaggat gagtaaatct cttacaaatc aaatagagct agaccaatat aggttagaaa
    15121 cagtatttaa taagataaat aactatttag attcatacac aataaaagat aaagttatac
    15181 aattagacaa aatatatgat aaaataattt ttggaataga aaataattta aaattagaag
    15241 atgaaaaatt agtaaaaatt ggagcattac tccataattt aagtccatta gctactatgg
    15301 atagaggtta tagtataact caaaaaaatg gaaaagtaat aaatagtata aaaggattaa
    15361 aaataaagga tagcatagat atagtattaa aagatggaaa tttagaatgt atgatagata
    15421 aaattgaaaa taaagaggga tagtatgaat ttaacctacg aagaagctta taaaagatta
    15481 gaaagtattt taagtgaatt agaatctaaa aatgctagct tggatgaatc tttaagccta
    15541 tatgaagaag gtataagcct ttataaacat tgtaataagc ttttagatga tgctaaattg
    15601 aaaataagta aatttaatca attaggaata gaagaagatt ttaaaataga ggaggaataa
    15661 aattggaatt taaacaatgt ctaaaagaaa aagcaagttt tgtagaaaaa gtacttaaag
    15721 aatatatgcc taaagaagaa gggtatcaaa aaactgttat tgaagctatg aactatagtt
    15781 taagtgctgg aggtaaaaga ttaagaccaa tacttacatt ggaggcttgt aaaatagttg
    15841 gaggaaatga agatgaagct attccttttg ctattgcaat tgaaatgata catacatatt
    15901 cactaataca tgatgatttg ccagctcttg acaatgatga tttaagaagg ggaagaccaa
    15961 caaatcataa agtatatgga gaagctatgg ggattttagc aggagatgct ttgttaaact
    16021 atgcttttga agtaatgctt gcaggctcaa tcaataaaga aaatccagaa aagtatctaa
    16081 atgctataaa tgaaattgcc aaaggtgctg gtatatatgg aatgataggt ggtcaagtag
    16141 tagatgtaga aagcgaaaat aaacaaattg aaaaagaaaa attagattat atacatatga
    16201 ataaaactgc tgcaatgatg gtaggatgta tgagagcagg agctactata ggtggagcaa
    16261 attttgaaca aatggaagaa ataactaaat atgcaaaaaa tatcggatta tcattccaga
    16321 tagtagatga tatactagac atagtaggag atgaagctaa gttaggtaaa aaggttggaa
    16381 gtgatataga aaatcataag tcaacatatc catctctttt aggtttagat aaatctaaag
    16441 aaattgcaca taatttgatt gatgaagcaa agaaaagtat agaaaaatta tctgatgatg
    16501 tagatttttt aaagggattg gctgaatata taatagatag agaatactaa aatatacagc
    16561 taaaatcgag gagggaaaaa tggatttttt ttcggagatt tttaacaatg gggctttggg
    16621 aataagtctg attgcttgtt tcttagcaca atttataaaa atatttactg gtaaggaaaa
    16681 aagaattgag ttatcaagaa tacttatttc aggaggtatg cctagttccc atagttcttt
    16741 tgttacaagt ttagctacag tggtaggtat tgaaaaaggg tttaattcta ctgattttgc
    16801 tataattacc gtgcttgcct taattataat gtatgatgca gcaggtgtta gacgggctgt
    16861 tggtaaacaa gctactatat taaatcaaat ggtggcagat atacagcatg gaaagcatat
    16921 agaacagaag aaattaaaag aattaatagg tcatacacca ctagaagttt ggtttggagc
    16981 tttacttgga atagtaacag ctttaatatt gatgtaaatt gaaaatttac agaataaagg
    17041 tgaggataat atgtataaat atttagataa agtaaattct ccaaaggata taaaaaatat
    17101 gagtatagaa gaaatggatt tacttgcaaa ggatataaga aagttcttag taaaatctgt
    17161 ttcaaagaca ggaggacact tagcttctaa tctaggagtg gttgagttaa cattagcact
    17221 tcataaagta tttgatagcc ctaaagataa gattgtatgg gatgtagggc atcaatctta
    17281 tgttcataaa atagttactg gaagaaaaga ttgttttgta tctttgagac agtttaatgg
    17341 actaagtggt tttccaaagg agaatgaaag ccctcatgat atttttgata caggacatag
    17401 cagtacatct atttctatag ctacaggtat tgcctgcgct agggatataa aaaaagaaaa
    17461 ttatagtgtt atatctgtga taggagatgg ttctataaca ggaggtatgg cacttgaagc
    17521 tttaaatcaa ttagggtata tagataccaa tatgatagta atccttaatg ataatgagat
    17581 gtccatagat aaaaatgttg gaggaatgtc taaatatctg tcaagtatta taagaaactc
    17641 aacagttgag aaaatgacag atgaagttga taaaatttta aatgttacac aaacaggtga
    17701 aattttatct aagactgcac atagatttaa agataaacta atgtatagct tttctcctca
    17761 ggactgttca ttttttgatt cattaggtat aagatattat ggacctatag atggacataa
    17821 tactaaagaa ctaatagata tattgagaaa agctaaacac aaaaaagggc ctgttctttt
    17881 acatgtaatt acgaaaaaag gaaaagggta cagatttgca gaagaacaac ctgataaata
    17941 tcatggagta tcaaagtttg atataaagac aggtgttaca tctgcaaaag ttaagtctat
    18001 gtcaattagt gttggagaaa agcttgttga tatggctaat aataatgaag atatagtagc
    18061 aataacagct gcaatgccat ctggtacagg gctaaaccta tttgaaagtg catatccaaa
    18121 aagatattat gatgttggta tagctgaaca gcatgcaaca ggatttgcag caggtcttgc
    18181 aaaaaatggt atgaaacctt actttgctgt gtactcatca tttttgcaaa gggcatatga
    18241 ccaagttatt catgatgtat gcataacaaa aaaaccagtt acttttctta tagatagagc
    18301 tggattagtt ggaaatgatg gagaaactca tcatggtatg tttgatttaa gttacttaaa
    18361 ttctattcca aatattgtag taatggctcc aaaagataca agagagatgg agcttatgat
    18421 ggatttgtca ttaaaattag attgtccact agcaattaga tacccaagag gaagtagtta
    18481 ttacctagat aagggagaat atggagagat tgtactgggt aaatatgagg tattagatga
    18541 tggtcaagat acagtcatac tttgtattgg aagtatggta aaacatgcct tagaggctaa
    18601 agaaatatta tcaagagaag gaataaatcc gacaatagtt aatgctagat tcttaaagcc
    18661 aattgatgaa gatatgttaa aagtattatt aaaaaatcat aaaaatgtag ttactataga
    18721 ggataatatt gtaactggag gatttggaag tagaataaat aagtttatta tagataatga
    18781 atataacgta aatatactta atatagcaat accagaagaa tttgtaaagc atggcaatgc
    18841 agatgaatta tatgattttg taggattatc gccaaaaagt attgctgata aaataagaaa
    18901 attagttatt gagtaaaaat ctaatgtaga tattgtaaat taaaacttat ataaagtata
    18961 gaatttatca attagaataa atatcaaatg tgttacttca agctacttta gttatcatta
    19021 aagtagcttt taacagaaag gtaattgtat gaaaaaaaga atagatttat tattagtaga
    19081 acaagggtac tttgaaagta gagaaagagc caaaaaagca ataatggctg gattggtttt
    19141 tgttgacaat caaaggtgtg ataaggctgg tacagaagta aaagaagatt gtagtataga
    19201 agtaaaagga aatccaatac catatgtaag tagaggtggt ttaaagcttg aaaaagcaat
    19261 gaaaaacttt gatttgacta ttgatggaaa agtatgtatg gatatagggg catccacagg
    19321 tggttttaca gattgtatgc tcaaaaatgg agcaataaaa gtattttcta tagatgttgg
    19381 ttatggtcaa cttgcatgga aattgagaca agatgatagg gttgtgtgta tggaaagaac
    19441 caatataaga aatgtgacaa tagaagatac aaagcagttt gctgattttg cgtcaattga
    19501 tgtgtctttt atatcattaa agttagtact tccaaaggca aaggagttag tacgtcatga
    19561 tggggaaatt gtcgctttaa ttaaacctca atttgaagca ggaagagaaa aagtaggtaa
    19621 aaaaggagta gttagagaaa aatctacaca tatagaagtt ataaaaatga tttctgattt
    19681 ctcagtcgaa aatggatttg agattttagg gttagatttt tctcctatta aaggtcctga
    19741 aggtaatata gaatatctaa ttcatttgag aaatggaaat gaaggatatg aatttgatgg
    19801 tgagatatac aataacaaaa tagtagaagt tgttgaagct tctcataatt tagacaaata
    19861 ggttaaaagg gggcttagat ttgatcctcg aattatatat gaaaaattgt gccttagttg
    19921 aagaactaag gttgaatatt gataaaaact taaatatact tacaggtgaa actggttcag
    19981 gaaaatctat aataatagat gcacttggac tttgtttagg tgataaatat gataggtctt
    20041 ttttaagaaa aggaacagat aagggacttg ttgaagctgt atttttttca gacaatatat
    20101 atcttaaaaa aatattagaa gaaaatgata taagtatgga agatgataat ttattggtaa
    20161 taactaggtt gatatactca gatggtaaga gtactgctag agtaaatgga agaactgtta
    20221 aagtgtctct tcttaaagaa attgcatcta cacttataga tatacatggt caacatcaaa
    20281 atcaagcttt atttaataaa gatactcatt taaaatttct agaccttttt ggagaaagtg
    20341 aattggaagg gtttaaaatt gcttataaaa atgtgtacca taagtatagt gaagtaaaaa
    20401 aagctttaaa ttgcttgaca gaaaataaag atgagatgca aattcaacga gaaatagatt
    20461 tattaagatt tcaaataaat gaaattgaag ctgcaaatct aaataagaat gaatatgaag
    20521 atttattaaa acaaagagaa gtttatagaa atagtgaaaa gatatataat aatctaaatt
    20581 cgagttatag caagttacat aatggtgagt ataatgttat tgacctaata ggtcttgcat
    20641 ctaaagaact taatgacata tctaaatatg acagtgtttt aagtgaatat agtgatactg
    20701 tagaaagaat tatgtatgag ttacaagaca tatctggtga gataagaaat tacaaagata
    20761 atatagactt tgaaccttat gagctagagc agattgagct tagaatcgac gagattaata
    20821 acttaagaag aaaatatgga gacagtatag atgatatatt tgagtattat ggaaaaatta
    20881 aagatagatt agatgaaata ttaaatagag atgaaagagt tgaacagtta aaaagcaaac
    20941 tgatgaatat agaagaagat ttaaaaataa aagccagtaa attgactaaa gcaagaatag
    21001 aagtagcaac tactttagaa aaaatacttc ttgatgaatt aaaaagctta aatatgaaaa
    21061 atgtaatgtt caaggttaat tttgaaaaag gtccatttac tttagatgga gtagatgata
    21121 ttgaatttat gatatctttt aacttaggag aagatattaa gcctatatat aaagttgcat
    21181 caggtggtga gatgtctaga tttatgttag catttaaaac catattggct gatatagatg
    21241 atattgatac attagtgttt gatgagatag atactgggat aagtgggata gcagcgcaga
    21301 ttgttggtga gaaattgagt gatatagcaa agaaaaaaca gataatatgt ataactcatt
    21361 tacctcaaat tgctgcaaat gcagacactc attattgtat agagaaagat acttcaaata
    21421 ataggacgtt taccaatgtt agtaagttaa atgaatctca acgaaaaaat gaaattgcca
    21481 gacttatagc aggtaataat ataacagaaa aaactattga acatgcaagt gaaatcattg
    21541 aattggctaa aaaatgttag tgaaaaacaa gcattttaat aggtgtaaag aatttttata
    21601 gaaatgcttt acacctattt ttttatgatg aaattatttt aaaatatcta gtgcctatat
    21661 tgaagaaaag tatgtttgaa aatgtaatga aatttgtata aatttgtaac tttgaaattt
    21721 ctaaatgctt aagtataata ttatttatta aggtagtagt gaggggaggc ataaaatgaa
    21781 taaaaaagat actatgcact taatttttat aattgtaatt tttattttat tatggtatat
    21841 tgtggtagca gtaagtgaga ttaataaaaa tattgaaagt tgtagtaaca aattagatta
    21901 tttatcacaa gagattatga gcactaagtc ggatattggt aatactataa atgaagaaat
    21961 gtcaaaaagt tatataacaa aaagtattga cttaaaagta aaaaaaatcg agaaaaaaga
    22021 gtgtattatt gatatagatg ttcaattaag taaacttgga aaaaatggaa aagtattctt
    22081 tatgtataaa gaagatagtg ataaatggaa tgaaactgag atgacaaaac atggagaatt
    22141 atcatatgca tgtgaaataa agataaatac aggaagtgaa tatgactata aagtagttac
    22201 aagtggagct atatctgaaa gttctgatgt gcaaaaattg actaaatctg attatatgcc
    22261 tgaacaacct atatttaatt ctgggataag agataataga gaatacttta ttgaaatagt
    22321 agaaaatagt aatcttttca atcatgagag tgaaaagtca aaaaataaag cttatgataa
    22381 tattagatta gaaaaagtag atattattgt aaatgaagga aaaaaagaca ctatatacaa
    22441 agccaaatta gctgaaggct tagatgatgg caaaactaat aattcaaata gaaatcaagt
    22501 gaattatgaa gtaagttttc ctaagaggga tattgatgac ataatttata ttaaagctaa
    22561 acttacgtac aataatggag tagaatatac aaaggatata actgaagata taactatggg
    22621 attacaagat ttagaaaaat aaatacatat ttaaataaat attcaaatag atttgttcaa
    22681 taaaatagag tataaataaa actgtagtag ttttacttat actctattaa aaaattataa
    22741 ttttaattcc atatatctag ccccttcaat tggattataa gtataaggtt ctatatcata
    22801 aaaaccaaac ttttcataca atccttgtgc acttttcatg cttggaagag tatctaatct
    22861 catgtgtgta tatccaattt ttttagcttc ttcaatgatt tcttccaata gtatttttcc
    22921 tatttttaat cctctaaatt gatttcttac atataatctt tttaattcac atattttata
    22981 ttcaagtttt tttaaagcaa cacaacctgc caaattttca tcaacaaatg ccaatatcaa
    23041 tgagcctgaa ggttttttat attttcctgg aagtgttttt aattcattat taaagtcttg
    23101 gaaacataag tctatattta gagactttga atattcagta aataaaagtt ttacgttttc
    23161 aaggttttct aaatcgtttt cttcaacaaa ttgtaaagtt atattttgaa ccattttatc
    23221 cgccattata taaacatgtt tatataatga cttcacctcc ttgatatatg tatatttttc
    23281 ttaatatcaa ttagatttta ataccattac tttttcacca ccatagtcaa gctctccatt
    23341 aaactcaaat ccaaaatctt tataaagctt aatagccatt ttattgtttt cgaatatact
    23401 aagatatagt tcatcacagt tatatttatt tttaagatga tttacaagaa aatctaatga
    23461 tgcttttcca tatcctttac cttgatattt tgaatcaatc ataaatctat caagccaaac
    23521 tctatcatct tctaaacgac catacatagc aaatcctatt aaagaatttt catcatatat
    23581 tccaacaggt acccaagctg ttataaattt agattcagca atagaaagtg cattgctttc
    23641 tatataatct aactgacttt tatctacaga caaattaact aaatcacgcc aattatttat
    23701 gtctgcaatt cttacattta atttcaagat attaccgccc ttcaaacttt ttgttacaat
    23761 ataatactat catattatgt attaaaaatc acttattaag attttaaata aactttttga
    23821 atgaataaat tctcaggaaa aaggaattat aaatacataa taatacagca taggagtaga
    23881 tggatatatg aacaaaataa ataaaaattt caaaaatatt tggataatat taggagctgt
    23941 atcgattttt accatgggta taatttttaa taaaaattgt aatgttaata aagaaactca
    24001 aatagaagct tttacaatta agaatcatga taaaaaattt gtatatccta tgggaaatat
    24061 aattggagta aaagcaaata cagatggtgt attggtctta ggatatgagg aagaggatat
    24121 agactatatt ggaggcatac aaatcggtga taatatagta aaaatagata ataaaagaat
    24181 aaaaaatagt caagatgtat cagagatttt gaataagatt aaaaaaagta aagttgaagt
    24241 gacttttgag agaaaaaata agtataaaac agaagtaata gagactaaac aagaaaatgg
    24301 caagtataaa cttggattat gggtaagaga taaaatatct ggaattggaa ctatgacttt
    24361 ttacgaccct agtatggaaa agttcaaagc aatcggtcat gccataaaag attcggatac
    24421 taatgaacta ttaaagataa agcaaggata tatatataag ccagaacaat taaaaatagt
    24481 aaaagctagt aatgaaaaag taggtaaaat aaaaggagat tttaatgaca gcaatttaat
    24541 gggtaatttc tcaaataatt cagagctagg tataagtggt aatattatag aaaaccataa
    24601 taaaaaattt aatgtagcaa ataaagaaaa gcaattaata gaagttggaa gaccacaaga
    24661 tgtaaaaata ggagatgcag taattctttt tgaggataaa aacaaaaata taacaagcta
    24721 tgatataaaa atagaaagta tagtgtatga taaaggaaat tatagagata tggtaataaa
    24781 agtagtagat gacaagttat tggaatacac aggaggtatc gtacagggga tgagtggagc
    24841 tccaataata caaaataata aaattattgg tgcaataact catgttttta gagataatcc
    24901 gaaaaaagga tatggtattt ttatagatga aatgataaaa ttgtaggtga ggcattaaaa
    24961 aattttatta ttttatcaat tatctaggag gaatataatt ttggagtgtc gaatatgctt
    25021 tagagtagat aattaggaag caattgtgta aaaagtttag ttttctgtaa taagaagatg
    25081 ttttttaatg gggggatttt tagtggaaaa aatcaaaata gttttagcag atgacaataa
    25141 ggatttttgt caggtattaa aagagtattt gtctaatgaa gatgatatcg atatattagg
    25201 catagctaag gatggaattg aagcattaga cttagtaaaa aagacacaac cagacttatt
    25261 aatactagat gtaataatgc cacatctaga tggattaggt gtaattgaaa aattgaatac
    25321 tatggatata cctaaaatgc caaaaataat agtactatca gcagtaggtc aagataagat
    25381 aactcaaagc gcaataaatc taggagcaga ttactacata gtaaagccat ttgattttgt
    25441 tgtgtttata aacagaatta gagagctagt ttctaataga gttacacaag ttgagccaaa
    25501 acctagacca gttcaagaga ctcaaatgac tagaagtgat tttgttaaga atgtaggaaa
    25561 tatagagaca gaaatcacaa atataataca tgaaatagga gtaccagctc atataaaagg
    25621 atatttatat ttaagagaag caataaaaat ggttattgat aatgttgagc ttttaggtgc
    25681 agtaacgaaa gagttatatc caagtatagc taaaaaattc aatacaacac caagtagagt
    25741 tgaaagagca ataagacatg caatagaggt tgcgtggagt agaggaaaag ttgacacaat
    25801 aaatcaatta tttggatata cggtacacaa tactaaagga aaaccaacta attcagaatt
    25861 tatagcaatg attgcggata aattaagact agaacatagt atggttaaat aaacaagaca
    25921 taaaaagtaa ggctttttaa ttaaggcatt ggctataaat gcgtattaca agcagcgaaa
    25981 cggtataacc actagggtta taccgtttcg ctattttaat aaatataaaa attttcttta
    26041 ttatttgctt actatatcaa tataataatt ttattatact atggatatag tatgtgtctt
    26101 tataagttgt aaactggcag tggtttattt tttaatataa atattgactt tggtgtaggt
    26161 aaactttgta tttttaagca tattatggaa tatgttaaat aaaaaaatga tgaaatatag
    26221 tattgtaaat gccaaagatg cgaaacaaac ttaaaacatt tattttattg ttaagtaatg
    26281 ctataatata atgtgatttt aataatgata gtggaggttt aaatatgaga gtcgaggccc
    26341 ctataaaagt agatcgaaaa accaaaaaac ttgctaaaag agttgaaagt ggggaaatag
    26401 cagttataaa tcatgtagac atagatgaag ttgctgcaaa ctctttagta gaagctaaaa
    26461 taaaacttgt cataaatgcg gctccttcta taagtggtag gtatcccaat aaaggtccag
    26521 ggatattaac tgaaaataat atactaataa ttgataatgt tggagaagaa ttatttgaag
    26581 aattaaaaga aggagaaact atagaagttg tagatggaaa gatatataga aaaggaaaat
    26641 ttttaggtgc aggagaagtt ttagacaagt atgaggtttc acaccaaata aaggctgcct
    26701 atgagaattt agctgtagaa ttggatagat ttatagataa tactatagat tatgctaaaa
    26761 aagaaaaagg atttatactt ggagaagtag aaattccaaa agtgaaaact aattatgcta
    26821 ataaacatgt attgatagtt gtaagaggtc aagactacaa agaagattta agtactatgc
    26881 tttcttatat agaagaggta aagccagtat tggttggtgt tgatggagga gcagatgctt
    26941 taattgagtt tggatatact ccagatgtga ttgtaggtga tatggatagt gtaagtgatg
    27001 aagctttaaa aaaggctagt gaaatagtag tacatgctta tacagatgga agagcaccag
    27061 gtcttaagag ggttgaggaa ttaggcttag atgcagttgt atttcctgcg ccaggaacta
    27121 gtgaagatat agctatgctt atcgcttatg agtataaagc tgagttaata gtagcagttg
    27181 gtacacattc aaatatgatt gattttcttg aaaaaggaag aaaaggtatg gcaagtactt
    27241 ttttagttag actaaaaata ggttcaaagc ttatagatgc taaaggcgtt aatttgttat
    27301 acagaagtaa attaaaaata aagtacatat gggcattaat agcaacagca ttatttcctg
    27361 tgttggtagt tgcatcattg tcaccaggag ttcaacagtt catacaacta atgcaattaa
    27421 agtttagagt attattacag atgtaaattt taggaggata aatatgcata ttaatatgaa
    27481 atattatata gtcactatag gggctatatt tatagctttg ggtataggca tgttagtagg
    27541 atttaattta aataataatc aacaattaag tgaacaacaa gcaaacataa taaatgattt
    27601 ggatgataag tttaatatct taaaagaaaa aaatgataaa cttgacagtg acttagcaag
    27661 tgtaaataga gattatgaag aggcagttaa ttttataaat aaaaatgtag ataagatttt
    27721 agctggttcg ctgaatggta aaagtatagg aataatatcg gctaatgaaa atgacgatta
    27781 tacaaaaaat atagaagaca taataaataa atctaatgga agtattgcat ttaatatagt
    27841 attaaaagaa aatatatcaa atccagaaaa gttaaaggaa atgtcaacta aactaggtac
    27901 agatgtaaaa aatgcaaatg atgcagttaa ttatataatt gacactctta aaaaggaaga
    27961 tgcaagtgac attttaacat atcttcaaga actagatgta ataaagttta actttattgg
    28021 agatacatat ctaaaatatg attctgttgt aatagctggt ggaaatgatg caaaagacaa
    28081 tacaaaacag tttgaaaaaa tagaaaaatt tgtagtttca aaacttaaat cagagaataa
    28141 atatttagta gaagttcaaa atacaggagt taaaacttct tatgtagaac tatattctaa
    28201 aaacaaagtt gcaactatag acaatattga tgaaggtata ggaagtatat ctctagctat
    28261 tcttctacaa caaggtaata tagttggtaa ttttggtaga ttggatactg caacttcatt
    28321 gttgccatct attaaataga aataatttta aggagagaaa aaatggattc ttatataagt
    28381 ataataatac cagcttataa tgaagaaagt agaataaaat atacattaga aagcatattg
    28441 gacattaagc aaataaatga aataatagtt gtggatgatg gttcgattga caatactgct
    28501 aaagttgtat ctgaactaga aaatgaaaag attaaatttt ttaagttaga taaaaataga
    28561 ggtaaaggat atgctttaaa ttatggtatc aagttagcaa tgaaaaatgc agatattata
    28621 ggatttctgg atggagattt aggaagctct gctaaagaag ttgaaaaatt aatagtacct
    28681 atattaaatg gtgaggcgga tgttacaata gccaaatttc ctcctgcaaa gagaaaagga
    28741 ggtttgggat ttgtaaaagg tttagctaaa aattctgtgt ttgagatgac tggtgttgag
    28801 ttagatgcaa ctttatcagg tcaaagaata tttaaaagag aagttttaga aaagtttgat
    28861 gaaataccct ttggatatgg agtagaagtt ggaatgacca ttgatatatt aaagtatgga
    28921 tttaaaatta aagaagtatt agtaaatatg actcataatg aaacaggaag aaatttaaaa
    28981 ggttttattc atagagggaa acagtattat cacataaaaa aggttttagg acaaaaaaag
    29041 aaagaatgga ggtaatatct tagtgaaaga ttatatactt tataccattt tattattaac
    29101 tggattattt ggaacctatg ctgtaattcc actatttaaa aatttattga taaatggaaa
    29161 tgtacttaga ccaaactata aaaaagacat gataccagta agtatgggaa ttgtattttt
    29221 gcctatgatt gtaatcaatg gaataatact tgctttcttt actacagaat ttaaggattt
    29281 agcgtatata tttatgttta tgtttggtat gatatccatg ttttttgcag ggatactaga
    29341 tgatgtcatt ggaaatagag atgtaagtgg tttaaagggg cattttaaaa gtttatttaa
    29401 aggaagtctt acaacaggag gttttaaagc tttatttggt ggatttgtag ggcttatagt
    29461 ttctgttgct atatcaaaag acataattga tataatagta aatacactaa taatagctct
    29521 atcaactaat ttaatgaatc ttttggattt aagaccagga agggcaataa aagcatattt
    29581 attcataatg gtaattatat ttttaacatt gacaggattt gttcaagttc ttccattact
    29641 tatagtacca aatgtacttg catactttaa ttatgattta aaagcaaggg ctatgatggg
    29701 agatactggt tcaaacgtcc ttggaatatc tattggaatg ctcatttctt ttgggtatcc
    29761 atttaacatt agattaggat ggttaatatt tgtagttatt atgcatatat tcactgagaa
    29821 atactcatta actaagataa tagaaaaaaa taaactatta aattttatag ataaattggg
    29881 aaggtagtat tatgataagc aaaagagtgg ggattgttga atcaatagta agtcaaactg
    29941 aaactctaga tgatataaga gtaaatataa atggagaaat acaaaaggca tataattatc
    30001 caaaaatatc tggaactata aatataggtg atgaagtggt tttaaataca acagcagtag
    30061 aactgagctt aggtacaggt ggataccatt ttgtaataac taatttaaat aatatagaat
    30121 ctactttaac tgaaggtgga catataatga aacttagata tacgcctctt caaataaaag
    30181 tggattctgt tgaggaacaa gaaagtattt atcatgataa atttgcgaat tttaaaggat
    30241 tagaaggtct ttctgttgta gtagggactc ttcatagtat gttaactcca tttgcagctt
    30301 catttaaaag aaataatcca aataaaaaat tagtgtatat aatgactgat ggagcatctc
    30361 ttccaattta tttaagtaaa aatgtggata ctttaaaaga aaaaaaatta atagatagta
    30421 caataactat aggaaatgcg tttggtggag attatgaatg tataaatata tatactgcat
    30481 taattacagc aaaagaaata ctaaaagcgg atgcagtatt tgtcagtatg ggacctggaa
    30541 ttgctgggac aggaacaaaa tatggattta cagggattga acaaggctct attcttgatg
    30601 ctgtaaaaaa acttgaagga agagcaattg caattccaag aattagtttt gctgacaaac
    30661 gagaaagaca tcaaggtatt tcacatcata gtatgactgt atttaaagaa attgtaaatg
    30721 taaatgtaga cataccaata actatatatg atgatgaaaa attgaacttt ataaaagagc
    30781 aaatcaattt aaatggttta gataaaaagc ataatataat ttatatagat aataataaaa
    30841 cgaaagagga tttagactat tttaatctaa aagtaaagag tatgggaaga aattatgaac
    30901 aagatgaggc atttttcaaa gctgcaagca cagcagcata ttacttaatg gaggtttaag
    30961 atggtattag aagaaaaaac tattagtagt gatagggttt atacaggtaa agtaattact
    31021 ttaaaggttg atactgttga aattccaggg caaggatatc aaaaaaggga gttagtagaa
    31081 gttggtggag cagtaggaat agttgcaatc acagatgata ataaagttgt attagttaag
    31141 caatttagaa aacctataga aaagccaatc tttgaaattc cagctggtaa actagaaaaa
    31201 aatgaaagtc caaaagaatg tgctgaaaga gaattaaaag aagaaactgg gtatagtgca
    31261 aaaaatatta agctaataca taaatttttc acttcagcag gtttttctaa tgagataatg
    31321 tttgtatatt tagcaacagg tcttactcca ggagagaata atttagatgc agatgagttt
    31381 ttagatgtct atgaaattga gcttgaagaa gcatataaca tggtattaaa aaatgacgtt
    31441 gaagatgcaa aaacatctat tggattatta ttagtaaaag atatgtttaa aaattaatat
    31501 ttataaaatg aataaccctt tgtcctaaag catactattt tataaaggac aagggggaat
    31561 taaattgaga agaacatata gaaaaaatga ttttagagaa ttaaatagac aaataattat
    31621 tataggattg ttgtttatga tgtctattgt tgttgggtct tacataaata aaatattgcc
    31681 aggttctagt aacaatatat taaacaatat aaatcctgct gtagagtatt ataatctaaa
    31741 tataagcata aaagatacag tgatacaaaa tttaaaatca gatgcaatct ttatgggctc
    31801 aatagctcta ttaagtctat ttgtagtaac aattccagca gtgttggtag cttttgtatt
    31861 aaaagggatg tcaattggtt atacaataaa tagttgtata ttggcattaa aatttaaaag
    31921 tataaaaatg attttaataa tattatttaa aaatttgatt attattccat gtgcaatcat
    31981 attagcatta attagtttaa gttattttaa agaaatggtt tatgaattta aaaagaaaaa
    32041 tagaaaaaat atgcaatttt taattaagag gtatatacta aatattataa ttataatagc
    32101 actatcattg gggttgcaat taatattaaa tactgcaagt attggtatta ttaagttcct
    32161 agctaaataa aggagtttat aatggatatt atagagggat atatagatta cataaaagat
    32221 aaaaaaaaat tatcagagaa tacagtggca tcatacttta tggatataaa aaaatatatg
    32281 gaatatataa atcaaaaaag cattaaacta gtagatatag tagaaaatga tattataagt
    32341 tacttaataa agttggaaaa agataatgta tcaatagcta caatagcgag gatgatatcc
    32401 tctataaaat cttttcatga ttatttattt ttaaatcata tatgtacaaa taatcctgca
    32461 aaggatataa aaaaacctaa aataaaaaaa gaaaatataa atatacttac agaagaagaa
    32521 attgaaagac tattaaattt tccaaagtta acaacgccta aattgattag ggacaaggct
    32581 atttttgaag tattatatgg tactggtata aaagtatctg aattagtaga gatgaatata
    32641 gaggatattg acctagatat tgattatata tattgtaatt catgttgtaa aaatcaaaga
    32701 gtgataccac tttgtgatat tacaaaactt tacttagaaa agtatctaaa agaagcaaga
    32761 cctaaaatgg ctgtagaagg tgaaaaatca ttattcgtaa gttcattagg tcaaaggttt
    32821 acacgacaag gtctttggaa agtcataaaa aaatattcta atctagccaa tatagataaa
    32881 aatataaatc ctacaatgct tagacattca tttgctattc atttgttaaa tgaaggtgct
    32941 aacattgctg ttgtaagtaa aattttagga aatgttaatt tatctagttt gcaagtatat
    33001 ttaaatcata tagataaaaa cgtgagaaga gaaataaagg agaaacatcc tagaaatgat
    33061 gttgatgtag aattacaaaa agcagaagct aaatagacta tatagcaaat tttataattt
    33121 agaaggagca tatttatatg agcagagtaa tttggattgt aattgatagt gttgggatag
    33181 gagcattgcc tgatgctgaa aaatttggtg atagtaagga tgtaagtacc cttggaaata
    33241 tatttaagga atatccagat attcagattc ctaatatgag aaatttaggt ataggaaaca
    33301 tagatggtat agattttttt gaaagtataa aagaaccaat aggttgtttt ggaaagtgta
    33361 aggaaatgtc tcagggaaaa gatactacta caggtcattg ggaaatgact ggtataatag
    33421 ttgacaaacc atttaagaca tttgaacatg gattctctaa ggagataata gaagagtttg
    33481 agaaaaaaac tggaagaaaa gtagtaggaa ataaaccagc ttcaggaact gtaataatag
    33541 atgagtatgg agagcatcaa ataaaaactg gagatgtaat agtatataca tcagctgata
    33601 gtgtatttca gattgctgct aatgaagaag taattcccct agaagaactc tataatatgt
    33661 gcaaaatcgc tagggaaata atgatggggg ataatgcagt agctagagta attgctagac
    33721 cattcatagg taagaaaaaa ggtgagtttg tgaggacttc aaatagaaga gattattcac
    33781 tagacccatt tgaaccaaca gttcttgata atataaaaga atcaggactt gatgttttag
    33841 cagttggaaa aatagaagat atttttaatg gaaaaggaat tacagatgct atccacacta
    33901 aaagcaatat ggatggagtg gatgaaactt taaattatat gaaacaagat aataaaggtc
    33961 taatatattc aaatctcgtt gattttgatt ctaaatatgg tcataggaga gacccagaag
    34021 gatataagaa agctcttgaa gagtttgata gtagacttcc tgaaataatg gctaatatga
    34081 gagaagatga tattttaata atcaatgcag accatggaaa tgacccaaca tataaaggta
    34141 cagaccatac aagagaatat atacctgtta tgatttatgg caataagatt aaaaaaggtt
    34201 ttaatttagg agtaaaagat acttttgctg atattggagc aactgttgca gatatactaa
    34261 atgtaaagtt gcctaaacat gggtcaagct ttaaagaaga tttattttaa actataaaat
    34321 tataaaaact tagcttaata ggatggagag attggaatgt ttgaaaagat agcacaaagt
    34381 agtaaattta taaattctaa aagtaatata aaacctaaga ttggtttgat attaggttct
    34441 ggactaggtg atttagcaaa tgatatagaa gaccctgtca caattaaata tagtgaaatt
    34501 ccaaacttcc ctgtatcaac agtagcagga catgctggac aacttgtaat tggaaaactt
    34561 gaaggaaaag aagttattgc tatgcaaggt agatttcatt actatgaagg atattctcag
    34621 aaagaagcta cttttccagt aagggttatg aaagaattag gagtcgagat tcttatagta
    34681 actaacgctg ctggaggcgt aaataaagaa tttaaagcag gagatttgat gcttataaga
    34741 gaccatataa actttagtgg aagtaatcca ttagtaggaa aaaatgatga cagatttggt
    34801 gctagatttc cagatatgtc agatgcatat tcttctaaat atgtagatgt agttaaaaac
    34861 tgcgctaaag aatgtaatat agatgtcaaa gaaggcgttt atatgttttt tagtggtcca
    34921 aactatgaaa caccagcaga agtgagaatg gcacaaattt tgggagctga tgctgtaggt
    34981 atgtctactg ttccagaagt catagtagct tctcactcta atataggagt tattggtata
    35041 tcttgtataa caaatatggc tgctggcatc ttaaatcaac cactaagtca tgaggaagta
    35101 atagaaacta ctcaaaaagt taaatcagaa tttatgaatt tagttaaaac aacggttaaa
    35161 aatttataag ttaaagctta ttatagattt tttgaggaag tttataataa aatagttaat
    35221 gtttaatttg aaaagaatta ataaaaagct gtcttgatat agaaagttat ttctaataaa
    35281 ggacagtttt tttataatac tacaacattt gaaagtgttt taaagtttaa tatttattta
    35341 aattattata ggttagaatg gagatttcaa ttatgagaat gtatgatatt ataaaaaaaa
    35401 agagagataa cctagagctt agtaaaaaag agataaacta ctttatagaa aactattcaa
    35461 agggagatat acctgactat caagcttctg cactattaat gtcgatttat ttaaacaaga
    35521 tgaataaaca ggaaactgta catcttacag aagcaatgat gaactctgga gacatgataa
    35581 acctatctga gatagatgga ataaaggtag ataagcatag tactggtggt gttggagata
    35641 agactaccat agctcttata cctttggttg cttcatgtgg agcaccagtt gcaaagatgt
    35701 caggtagagg tcttggtcat actggtggta ctattgataa attggagtct ataccaggtt
    35761 tttcgacaga aatggagata agtaaattta ttgattctgt aaataagtct aaaatagcag
    35821 tttgtggtca aagtgcaaag gtagcaattg cagataagaa aatatatgct cttagagatg
    35881 ttacagcaac tgttgataat atatcattga tatcatcaag tgtaatgtct aagaagttag
    35941 cttcaggagc tgatgcaatt gtgctagatg taaaaactgg tgatggagca tttatgaaga
    36001 cattagaaga gtcttttgag ttagctaaat ctatggttga tataggtact ggtatgaata
    36061 gagatacaat agggattgta acagatatgg atgaaccttt aggttttgca gtgggaaact
    36121 ctttagaagt tatagaggca attgagacat taaaaggaaa tggacctaaa gaccttgttg
    36181 agctatgcga agtattagga gcatatatgt tggttttatc aaaggtagca catgattttg
    36241 aagatggtgt tgaaaaaata agagaatcaa taaaatctgg tactgctata caaaaactca
    36301 aagagtttat agagaatcaa ggtggtaata aagatgtagt agatgattat tctttattca
    36361 aaaaatcaaa gtataaatat ccagttaaat caactaaatc aggttatata agtaaaatta
    36421 aagctgaaga tgttggtgta tcggctatga ttcttggagc aggtagagaa actaaagatg
    36481 atattttaga tttatcagca gggataattt tagaaaagaa agtaggagat tatgtaaatg
    36541 aaggtgaagt tttagcttac atgtattata atgatgaaca aaagctttca ttggcaaagg
    36601 gaagatttat tgattcctac tctattgtag attcaaaaat agaaaaaaat aaattaatat
    36661 atggtattgt aactaaagaa gagattaaaa agttttagaa aattttagaa aagtgattat
    36721 aaaaggccta acatgtcaaa aataatgt
//
LOCUS       DAEORZ010000027        35872 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEORZ010000027 DAEORZ010000000
VERSION     DAEORZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.027"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..1229
                     /locus_tag="KRH39_001196"
     CDS             <1..1229
                     /locus_tag="KRH39_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8511115.1"
                     /translation="IISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDL
                     IWAYLKEDMNKEAEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKV
                     NTYLILSKNIYKSFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKS
                     SLNLRKISIFYGLIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYE
                     LRQKQESLRLLVDDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDI
                     DCFKSYNDNYGHLAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVE
                     EIDNIELYIKRVFKLLEFEGIEHIYSKIKNYITISAGVSIKRCHSRKDVEILIEDADK
                     NLYKSKKAGRNQYIII"
     gene            1237..3237
                     /locus_tag="KRH39_001197"
     CDS             1237..3237
                     /locus_tag="KRH39_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8511116.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            3499..4758
                     /locus_tag="KRH39_001198"
     CDS             3499..4758
                     /locus_tag="KRH39_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG8511117.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            4819..6606
                     /gene="iorA"
                     /locus_tag="KRH39_001199"
     CDS             4819..6606
                     /gene="iorA"
                     /locus_tag="KRH39_001199"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBG8511118.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            6608..7183
                     /locus_tag="KRH39_001200"
     CDS             6608..7183
                     /locus_tag="KRH39_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBG8511119.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            7236..8306
                     /gene="buk"
                     /locus_tag="KRH39_001201"
     CDS             7236..8306
                     /gene="buk"
                     /locus_tag="KRH39_001201"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG8511120.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            8524..9225
                     /locus_tag="KRH39_001202"
     CDS             8524..9225
                     /locus_tag="KRH39_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511121.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHANN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            9285..9791
                     /locus_tag="KRH39_001203"
     CDS             9285..9791
                     /locus_tag="KRH39_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBG8511122.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            9966..10988
                     /locus_tag="KRH39_001204"
     CDS             9966..10988
                     /locus_tag="KRH39_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG8511123.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVTTRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(11135..11443)
                     /locus_tag="KRH39_001205"
     CDS             complement(11135..11443)
                     /locus_tag="KRH39_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG8511124.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(11503..11931)
                     /locus_tag="KRH39_001206"
     CDS             complement(11503..11931)
                     /locus_tag="KRH39_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511125.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(12154..13611)
                     /locus_tag="KRH39_001207"
     CDS             complement(12154..13611)
                     /locus_tag="KRH39_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG8511126.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            14029..14943
                     /gene="nadA"
                     /locus_tag="KRH39_001208"
     CDS             14029..14943
                     /gene="nadA"
                     /locus_tag="KRH39_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBG8511127.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIIEDEIMEKALNSLLNMHKLAEG"
     gene            14956..16257
                     /locus_tag="KRH39_001209"
     CDS             14956..16257
                     /locus_tag="KRH39_001209"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBG8511128.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            16238..17074
                     /gene="nadC"
                     /locus_tag="KRH39_001210"
     CDS             16238..17074
                     /gene="nadC"
                     /locus_tag="KRH39_001210"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBG8511129.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            17330..17662
                     /locus_tag="KRH39_001211"
     CDS             17330..17662
                     /locus_tag="KRH39_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBG8511130.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            17824..18009
                     /locus_tag="KRH39_001212"
     CDS             17824..18009
                     /locus_tag="KRH39_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511131.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            18036..19121
                     /locus_tag="KRH39_001213"
     CDS             18036..19121
                     /locus_tag="KRH39_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG8511132.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            19114..19572
                     /locus_tag="KRH39_001214"
     CDS             19114..19572
                     /locus_tag="KRH39_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511133.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            19607..20698
                     /locus_tag="KRH39_001215"
     CDS             19607..20698
                     /locus_tag="KRH39_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8511134.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            20703..20927
                     /locus_tag="KRH39_001216"
     CDS             20703..20927
                     /locus_tag="KRH39_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511135.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            20937..21500
                     /locus_tag="KRH39_001217"
     CDS             20937..21500
                     /locus_tag="KRH39_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511136.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            21461..22090
                     /locus_tag="KRH39_001218"
     CDS             21461..22090
                     /locus_tag="KRH39_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8511137.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            22130..22267
                     /locus_tag="KRH39_001219"
     CDS             22130..22267
                     /locus_tag="KRH39_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8511138.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            22267..23019
                     /locus_tag="KRH39_001220"
     CDS             22267..23019
                     /locus_tag="KRH39_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8511139.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            23032..23517
                     /locus_tag="KRH39_001221"
     CDS             23032..23517
                     /locus_tag="KRH39_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511140.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            23524..23673
                     /locus_tag="KRH39_001222"
     CDS             23524..23673
                     /locus_tag="KRH39_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511141.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(23810..24631)
                     /gene="yidA"
                     /locus_tag="KRH39_001223"
     CDS             complement(23810..24631)
                     /gene="yidA"
                     /locus_tag="KRH39_001223"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBG8511142.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            25124..26818
                     /locus_tag="KRH39_001224"
     CDS             25124..26818
                     /locus_tag="KRH39_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBG8511143.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      27150..27260
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            27364..27735
                     /locus_tag="KRH39_001225"
     CDS             27364..27735
                     /locus_tag="KRH39_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBG8511144.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            27831..28778
                     /gene="trxB"
                     /locus_tag="KRH39_001226"
     CDS             27831..28778
                     /gene="trxB"
                     /locus_tag="KRH39_001226"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG8511145.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            28882..29199
                     /locus_tag="KRH39_001227"
     CDS             28882..29199
                     /locus_tag="KRH39_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBG8511146.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            29468..30754
                     /locus_tag="KRH39_001228"
     CDS             29468..30754
                     /locus_tag="KRH39_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG8511147.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPNLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            30866..31339
                     /locus_tag="KRH39_001229"
     CDS             30866..31339
                     /locus_tag="KRH39_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:30998..31000,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBG8511148.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            31371..32681
                     /gene="grdB"
                     /locus_tag="KRH39_001230"
     CDS             31371..32681
                     /gene="grdB"
                     /locus_tag="KRH39_001230"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:32418..32420,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG8511149.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            32937..34469
                     /locus_tag="KRH39_001231"
     CDS             32937..34469
                     /locus_tag="KRH39_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBG8511150.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            34480..35625
                     /locus_tag="KRH39_001232"
     CDS             34480..35625
                     /locus_tag="KRH39_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBG8511151.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVEILVKAGYTAK"
BASE COUNT        14038 a         3855 c         6632 g        11347 t
ORIGIN      
        1 gtataataag tttgtgttat ttagagagtg gaaataatgt agaagctata aaatacataa
       61 ataatgtatt agatgttgca cacagtaata actcaatatc agtaactgaa agagtgtcta
      121 taaaaataga ccttatatgg gcatatttaa aagaagatat gaataaagaa gcagaagtta
      181 ttttattaaa gactataaaa ttaatgaaag ttgttgaagg tgtatataag gattatttat
      241 attcatttat atttttatat tcagctgaaa ttgagttaaa gaaagataat tgtagttttg
      301 aaaaggtaaa cacctattta attttatcaa aaaatatata taaaagtttt ggagatgttt
      361 ttatatataa ttctaaacta gattacatat ataagttgta tggagattta tatataaaat
      421 ttggtaatat agatgaaggt ataagttgtt atataaaagg tttaaattta gttaaaaaga
      481 gttcattgaa tcttagaaaa atatctatat tttatgggct aatagcaaat ggatatgagc
      541 taaaaaaaga ttttaaactg gcaatagaat attataaaag tatggattgg tattctgata
      601 ggtgggaaaa aaataattcc tataaaacat ctcaagcaat tcacaaacaa tatgaactca
      661 gacaaaaaca agagagttta agattactag tggatgataa taagaaatta gaaaatgata
      721 ttttaaaaga tggacttact aaactatata atagaagata tttggaacag cagttggatt
      781 tatttaatgg aaccagagac aaaaatatgg ttgcaatact ggtcgatata gattgtttta
      841 aaagttataa tgataattat ggtcatctag caggggataa agttatagtt aaggtaactg
      901 atattataag aagtgtattt atagatatta ccgaacacat aatcagatat gggggagaag
      961 aaattttaat attagtagat tctatagaat caatcggtgt tgaagagatt gataatatag
     1021 aattatatat taaaagagtt ttcaaacttc ttgaatttga gggtattgaa catatatatt
     1081 ctaaaataaa aaactatata actataagtg ctggagtaag tataaaaagg tgtcatagta
     1141 gaaaagatgt tgaaatatta attgaagatg cagataagaa cctatataaa tcaaaaaaag
     1201 caggtagaaa tcaatacatt ataatttaaa attgaggtga ttttgttgcg aaaaaaaata
     1261 ggaattatag cctctgatat tgaattaaaa gaaagaatag aagaattata tagagaagat
     1321 gtagaaaatg gaactataat aatcgacata cttaacctag acttaatgga aaatcaaggt
     1381 cgtatacttg ttgaaaaagg tgcccaagct ataattggta gaggtggagg ttatagttta
     1441 gtaatagata cagtaaatgt acctgttata ccaatgaata tgaagtcaac tgacctttta
     1501 agagcaatag aaatagcaaa gaaatattct aaaaaagtag tattgattct tggagacaat
     1561 gaagtttctt ttgactatgt tggctggaga aatgtcataa gtactgaaat aactgaagaa
     1621 tggtttgaat ctaagtatga gataagaagt aaagttgtaa aatatataga ccaaaaagat
     1681 gaagttgtaa tcgtaggagg aggtttggca tgttcttttg caaggcaata tggaattgat
     1741 agtgtatttg caactgctag tgatgaatct ataagagaag ctgtagaata ttgtaaaaaa
     1801 ttattagata cattaggtga agaaaagttt aataatgaag tgttacgaaa tatacttgat
     1861 ggtataaaag atggagtaat tgctatagat agtaatggca gtataatttt gtacaatgaa
     1921 agtgcaaaaa acatgcttaa agtagagaga aaatgtgcac ttaataaata catcttagat
     1981 gtatttccta aaatggagtg gatgttagat tgtttacatg aaaaagagga cgtagaagat
     2041 agaaagataa gaaatataaa taatctaatt gttaatacta ggacaacatt gataaaagtt
     2101 gataatagta cttatggagt tttaggaata atacaagata taaccaagtt acaaaactta
     2161 gaaagaaaaa taagatttga cttgaatcaa aaaggtctct atgctagata tacttttgat
     2221 gattttttat ttaaagataa attaactaaa gaatttattg aagaagctaa aaaaattgga
     2281 aaaagtgatt atacaacttt actttatgga gaaagtgggt ctggtaaaga aattatagct
     2341 catagtatac acaatataag taaaagaaaa gataggcctt ttgttgccat aaattgtgct
     2401 actatagcag aaaatttact tgaaagtgaa ctttttggct atgaagaagg tgcatttact
     2461 ggtgctagaa agggtggaaa aagagggcta tttgaacttg ctcatggagg gactcttttt
     2521 ttagatgaaa taaatagtct atcctttaat attcagacaa aactattaag agtaatagag
     2581 gaacgtcaaa taatgagaat tggttctgat tatataatac ctctggatat aaggattata
     2641 gcagcaacga acgaatcttt aaccgaaaaa atagttatgg gtacatttag agcagactta
     2701 ttttatagat tgagtagttt agagataaat atacctcctt taagagacag aagagaagat
     2761 ataatacctt tgtttaacaa ttttgtaaat gaagtattaa aggatgatgg tctcaatgga
     2821 ataaacagta tagatgaaaa ttttgttttg actaaagatg aaatggataa gctatataat
     2881 tatagctggc ctggaaatgt aagagagtta aagacaatag ctcaaaaata tgttgtgaca
     2941 ggtaaaatta aattgagaca agatagagat tttaaaacta aaaaattact tcctaactct
     3001 gaagtagaca aatttaattc tgaaacgaca gcaagtgtag aagtccaaga tgaatctata
     3061 aatataagta agataaatga tggtaagata agtatagata taaaagaagt aaataagtat
     3121 gttgaagaaa aaataataag tatgcttttt gctcaaggac tttctaaaaa tgaagttgca
     3181 caagttcttg gaataagcag gacatcttta tggaagaaat acaataaaaa tatttaaata
     3241 taattacata taaattattt tgaagtaatg ttaatttttt aaaacaagtg ttaagaaaaa
     3301 ttaacattac ttttttgcaa atttttattt ttttagacag gcatatatat ttataagaaa
     3361 aatagttaaa acacttaatt taataatgaa aaaaataata attctaaaca aaatcaattt
     3421 tacataaagt gggatttatt ggcatagtaa ttgcttaata tataatgtaa gagtaaatac
     3481 aattttaggg gtgatggtat gagtcaaagt atggttggta agcatgcaat gtggccaaaa
     3541 gaaaatgatg ttatatttag catatctggc agagctcaag cagcagagaa ggcttttggt
     3601 atggataatg taataaatgc aacaataggc gctctaatgg atgattcagg aaaattaata
     3661 acaatgaaaa ctgtatatga agaatataaa gcattagata actgtgagat aggtgcatat
     3721 gcagctcttg aaggtcaacc agattactta gaagcagtta aaaaagtatt tttcagagat
     3781 tacttaccag aaggacatat aagagtatta gcttcaccag gaggaagtgg tgcaataaaa
     3841 ttagctgtat ggaactatac taatgaagga gatgaggtat taacatctga ttggttctgg
     3901 agtccatatg taagtatagc tgaagaggct aatagaaaag tagttaatta ccaacttttt
     3961 gatgaaaata gaaggtttaa ttttgagtcg tttaaagaaa aatttgtaaa tatagcagaa
     4021 aaacaaggta gagtctttac aattataaac acaccagcgc ataatccaac tggatatagt
     4081 gttgcagatg atgagtggga taaaatatta gacttatcaa aagaagtcgc aaaagataaa
     4141 gacaaaaaga taatattttt cgtagattca gcatatattg actttgcagg tgatgatgat
     4201 gtatgtagaa agttttttaa gaaattttct aatttgcctg aaaacgttct cgtattggtt
     4261 ggattcagta tgtcaaaagg atttacagct tatggtatga gaatgggggc tataatttgt
     4321 atatcttcaa gtgaagatgt agctgaagag ttccattact catgtgttca ttcatgtaga
     4381 gcaaattggt caaattgtaa tagaagtgct atggcagtat tatctaacat tgtaaatgac
     4441 cctaaaaaat tcaaagaata tgaggatgaa aaagaaatat acaaaaacat gcttactaga
     4501 agagcagatg tatttgtaaa agaagctgaa agagtaggat tagaaattct tccatatata
     4561 gcaggtttct ttgtaagtat accatgtgat aatccaaaag aagtatgtga agaattgact
     4621 aaacataatt tatttgcagt tccattaaaa atgggcttaa gatttgcagt ttgtgcagtt
     4681 tctgaagata aatgtaagaa agcaccaagt attataaaag aagcacttga aagtttagaa
     4741 gtaaaaataa ataattaatt tcattgaaca aattgggtgg actatgaatt taataattgt
     4801 agatggaggg aattttatat gaaacaattg atgacaggta atgaagcaat tgcacgtgga
     4861 gcctatgagg ctggtgttaa atatgcatct gcatatccag ggactccaag tacagaaata
     4921 ttagaaaata tagcaacata taaagatgca atagttgcag agtgggctcc aaacgaaaag
     4981 gtagcacttg aggctgctat agggggttca attgcaggag caagaacaat ggcttctatg
     5041 aaacatgttg gtttgaatgt tgcagctgat ccaatattta cttatgcata tacaggggta
     5101 aatggtggta tggtacttat tacagcagat gagccgggaa tgcattcatc tcaaaatgag
     5161 caagacaata ggatgtatgc aaaatttgca aagatacctt tatttgagcc atcaactagt
     5221 caagaagcta aagatatgat aaaagaagct tttgaagtta gtgaaaaata cgatacacca
     5281 gttttatata gagttacaac aagactttgt cattcaaaag gattagtgga gtgctatgat
     5341 agagaagaag ttgaaattaa agagtatgtt aaaaatgcta agaaaatggt aactgttcca
     5401 gcaaatgctc aaataagaag gggcgttgtt gaagaaagaa tggaaattct taaaaaattc
     5461 tcaaatgaaa ctgatttaaa ctactatgaa ataaatgata caaaaatagg tgttatagcc
     5521 tctggtatgt gctgtaattt tgccaaagaa gtttttggaa aaaatgcatc atatatgaaa
     5581 ctaggattta caaatccatt accttatgaa aaaataaaag aatttgcaga aaaagttgat
     5641 aaaatatatg ttatagaaga aaatgaccca tttatagaag aacaaataaa agcatatgga
     5701 atagattgta ttggaaaaga tgtaatacca ccatatggag aaatgacacc agatgtttta
     5761 agaaaagcta tatttggaaa aacaaatgat actatagaat acaaatctga attagtaaca
     5821 ccaagaccac caagtttctg tgcaggatgt ccacatagag gattcttcta tgaacttgga
     5881 aagagaaaaa atttaatagt tggtggagat ataggttgtt atactcttgg atttgctcct
     5941 ccatataatg gaatagatta tgttgtttgt atgggttctg cttttggaac tgcacatgga
     6001 gctcaaaaag ttcttaatat gaaggatgat aatgaaaaaa gattggtagg tgtacttgga
     6061 gactcaacat tcttccatac aggaataaat ggacttttag atgttgttta caatagaggt
     6121 aattctattt cggttatatt agataataga ataactggaa tgacaggtca ccaagaaaac
     6181 ccaggttcag gatatacttt acaaggtgct aaaactaaag aagttgatat agaaggatta
     6241 gttaaagctt gtggaataga acatgtaaga gttataaacc ctaacaattt aaaagaagta
     6301 aatgaagcac tagattgggc tttagctata gaagatgaac catcagtaat aataacaaga
     6361 tggccatgtg tacttaaaaa attctctaaa gaagatatag aagaatttaa taatccattt
     6421 aagactaagt gtaaagttga ccatgataaa tgtataggat gtaaattatg tttgaaaact
     6481 ggatgtccag ctctatcatt tgataaagag aataagttat caaatataga tagaaatcag
     6541 tgtgtaggat gtggagtatg tgcacaagtt tgtccaaaac aagcaatagt taaggaggaa
     6601 aaataatatg actaagagta tacttttggt aggtgtagga ggtcaaggaa ctatacttgc
     6661 tagtaaatta ttaactatgg gattgatgga agctggatat gatgtaaaga tgagtgaaat
     6721 acatggaatg agtcaaagag gaggttcagt ttcctcacaa gttagatatg gagattgtgt
     6781 tcattctcca gttatagaaa ttggtggagc agatatatta gtatcttttg aaaaaatgga
     6841 agctttaaga tggttcaatt atttaaaacc agaaggaaaa gctttagtaa ataatcatag
     6901 gatagattct atgactgttt taatcggagg agctgaatat caagaaagtg atatagatgc
     6961 agagttaagt agattaaatg caaaagttat aaatgcagct gataaagctg aagaactagg
     7021 aaattcaaaa ataatgaatg ttattttatt aggatgtcta gttaaatcta tgcaattaga
     7081 gtctattgat tgggaaaaaa ttataagtga aaatgttaaa ccaaaatttg ttgaattaaa
     7141 cataaaagct ttccatgaag gtatggaaat ggttagcaaa tagtttataa tagatataaa
     7201 tagactatag aaattaaggt gaaaggaaga aaattatgac ttacagaata ttagccataa
     7261 atccaggttc tacttctaca aaaatagcag tatatgatgg agaagaacaa attcttgtga
     7321 agacgataga ccatccagct gaagagattg caaaatataa tactatacaa gaccagtttg
     7381 aaatgcgtaa ggaagcagtt ttgaatattc ttaaagaaaa tagtatagac ttaaaatctc
     7441 ttagtgcaat agtaggaaga ggtggagttt taccaccagt aaaatcagga gcatatttag
     7501 taaatgaaga aatgattgat gtactaagac atagaccagt acttgaacac gcttccaatt
     7561 taggtgctgt tgtggcacat gcaatatcag aacctcttgg aatcaactca tatatttatg
     7621 attctgttgc agtagatgag cttatagatg tagcgagaat atctggactt tgtggaatgg
     7681 atagatcaag tgcagggcat gcattaaata ctagagcaat ggctttaaaa tatgctaagg
     7741 ataaaggaaa agattataag agcttaaact taatagtagc tcacattggt ggaggagtaa
     7801 gtatttatct tcatgaaaaa ggaagaatgg ttgatatgct atctgatgat gaaggaccat
     7861 tttctccaga aaggtcagga agagtacctg ctacaaaatt agtggctgcc tgttattcag
     7921 gtcaatattc agaaagagaa atgactaaaa agataagagg taaaggtggt atagtttcat
     7981 acctaaatac tgtagatgct agagaagttg aaaaaatgat agcagaagga aatgaagaag
     8041 caaaaattat ttatgaagca atggcttatc agttagcaaa aggtattgga gagttagcaa
     8101 ctgtagtaga tggaaaggta gatgctataa ttataacagg tggaattgca tattctgaaa
     8161 tgtttacttc aatggttaaa aagaaagttg agtttatagc accagtagaa attatggcag
     8221 gagaaaatga gttggaatca cttgcttttg gaactttaag agtactaaat ggagaagaag
     8281 aagctagaat ttatagtgaa aattaataag aaaaactaaa aatgtttata tactaaaaaa
     8341 gtggtatatc cgttttttat acacgaaagc ttagagtata ggtgaaaatg tataaaattt
     8401 ggattgccac tttttataat taaaatttta tcttaaccta ataaataatt ctgtaaaatt
     8461 tcgtatttat atatatattt tattggtatt tattgttatt attattatga ggagtgattt
     8521 ttgatgggaa aaataagttt aaaaagtatg ataagattat tttcaggttt ttttatattt
     8581 gcaatttctt ctgtgcttat gattaatgca catgtaggtc ttatgccttg ggatgtactt
     8641 caccaaggtt tatctataaa gctaggtata actataggtc aggcttctat aatggtagga
     8701 gttgtaattg ttatattgga tgcagttttt ggtgaaaata taggatgggg tactttactc
     8761 aacatgacat ttataggaat ttttattgat ttagtaatat tttctggagt gatacctcat
     8821 gcaaacaaca cttatatagg tgtgtttatg gttgttatag gaattatttt ggcagctata
     8881 gcaagttttc tatacttagg tgtttgttta ggaagtggac cgagagatgg gcttatgatt
     8941 gctcttcaga aaaaaactaa taaatctgta cgtcttgtta gaacaattct tgaaatacta
     9001 gcattagttg ttgggtggtt attaggaggt tctgtaggta ttggtacgct tgtaagtgct
     9061 ttaggtttag gatatgtatt acaaatagtg tttagaatat ttaaatttga cacaaaattg
     9121 ttgaaacata gatttattat agatgatata agagaatgga aggaaaagaa aagcaatgaa
     9181 cataaatgta aatcaagtat tgtaataaaa aatgagcaga attaattatg ctaaaatata
     9241 tttgtagttt atctaattta tagtaaagtc aaggagattt tgaaatggaa ttatgggatt
     9301 tatataatgc tgatggaatt aaaacaggaa atgttataaa aagaggtaat tctattgaag
     9361 aaggttatta ccatttggca gttgaagtgt ggattttaaa tagtaactct caaattttaa
     9421 ttcagaaaag gtctaaaagt aaaaagacat tgccaaatat gtggggaatg acaactggat
     9481 gtattgtttc tggggaagac agtttagaag gagcaattcg cgaagcaaag gaagaaatag
     9541 ggatagatat aactaaagat gagatgaaag tttttagaag tatgattcat gaagatactc
     9601 tttgggatgt atacttagtt aaaaaagaat atgatatatc caaagctatt ttacaggagg
     9661 aagaagtaag tgatataaag tgggtaagca cagatgaaat aagacaatta ctaaaagagg
     9721 gtttgttttt tgaatatcca gagatatacg aattgctata tgagatagat aataataaat
     9781 taaatatatg atgtagagca gatttcagta taagtaagat tatttaaaat gtatcttctc
     9841 taaattttaa tattgtttta tgaaaattaa taagttattt ttagtatttt tctacttgtt
     9901 ttttgagatt aaaataaaaa gggcagtcat tataaagtca taaaaatttg gggaggattt
     9961 aaactatgga agataacaaa ggattggcac gtgacagttc aaaatataaa attgtttcta
    10021 tatctgtcgt agtaattatt ctaattacat tttggtatat gttaaatatg gtgcttttaa
    10081 catttattat gacttttatt ttctataact tgcttgtaac cacaagaaaa cgaattaaga
    10141 agttttcttc tttaaatatt ccagattcat tgataattat agtgttatat gcactatttg
    10201 ctatattgtt agtattgatt agctatgcag ttgtaccaat aattatagtt caacttacag
    10261 agttgagtag ggtttttagt gattttgatg taaatcaatt tgctcaatct ttaggaccaa
    10321 aattgtatcc tattgtctca aagctggatt ttaataaata tatttcacaa gcagggctac
    10381 ttatagcatc aacagcaaca aaagttggaa gttttggagt aaatatatta ttggcatttt
    10441 tactgagttt actcctttta cttgaaaaaa atgaaattaa aaattttgga gataaacttt
    10501 ctgatagtaa gatttctttt atttataatt cgcttgtatt ttttggaaag agctttgtta
    10561 aaaattttgg tgaagtaatg aaagtacaag ttatgattgc ctttataaat tctgttgttt
    10621 ctatgatttt cttaggtttc atgggattcc ctcaaatatg ggctttagga tttatgattt
    10681 ttgtgttggg tcttattcca gtagcaggag ttatagtgtc tttgattcca ttaactgtaa
    10741 tagcatttaa tacaggagga ataacaaagg tttttggagt tttacttatg atttgtatag
    10801 tgcatgcagt agaaacatac atattaaacc ctaaattgat gtcgaataga actaaacttc
    10861 cagtttgttt tgtttttatt attttacttg taggagaaca ttatttaggt gtatggggat
    10921 tactaattgg agttccaata tttatgttcc ttatggatat tctgggtgtt aaatttactt
    10981 ctaggtaata tattaaaata ataatttatt tttatatata aattatgaat tgttttaaaa
    11041 aagaacctat aaatagacat cattaataaa gacatctatt tgataggttc aattttgctt
    11101 tgtataaata ctataaaact aaataaaaag aaatttattt catttcaaaa gtttcacaac
    11161 ttgcatttct ttcatttata tgaacttttt cagctttgca aagttcattt ttattgtatt
    11221 tacattttac agcttcacaa tgaatgttat ctgtatcaac ttggttagtt tcattagaac
    11281 gactagttat accagatgta tatttgtctt caaaagaagc acaataagta ttttctgtag
    11341 tagttgcttg cattccatct acttttatac taccagcata acaatatcca ctagtattat
    11401 aagcacaatt agtagcagca caatttaaat tattattttt cataaataac ctcctaagtt
    11461 ttatttaaac ttattatgtg aagttatcta aattttatac acttatttac aattacattt
    11521 atcacattta acaactacat ctttggaagt ttcccttcct aaatttttat taacatgata
    11581 cacatcaaca ttttcagttg atccacatat agaacattta aaattgtttt ctagtagggt
    11641 tctttcttta aataacttcc aatacataga attgatgtaa tcattataat ttaccttgag
    11701 gcatgtctca agaaagttta tgtatagatt gtctaaatct cctgtattta attctgtata
    11761 ttctggttca aaatattcac aacatcctct aaaatcgggc tttgtatctg aaataacaca
    11821 tctaatatca tatctatagt ctaggctagg ccccttaaat gaaagatgtg tgcaaaaaga
    11881 acaagatgtt tctggtagat ttttttcaag ttcatgtaat ttattattca taagtattcc
    11941 tccaaaaaat ttattttaag tatataaacg aatagattat tttaaaactt ttatttattt
    12001 atattagaga tttaggataa atattccaat aaggagtaaa tacttaagta tttattactg
    12061 tattaaataa aaaagaggca aatctaactt gagattgcct acttttttat cattttttaa
    12121 taaaatttat tctaagtatt attttattta gatttagtca gccaacttag tatctgcttt
    12181 gctttttaac tttctaccag agcgtacagt taaaatagca tataatacag taaatatggc
    12241 agctaaaaca tatgatatat ttatgtttaa gttaaatcca atttgagcat taaatatata
    12301 actgaatata acaaaggaat aaaacatacc aggaattaag cttataatat aattcttttc
    12361 ttttattata aggtaaactg taatcattgc aaatgcaaat atggcaatag tttgattact
    12421 ccaagagaag tatctccaca atatattaaa tccagaagca ctaagctttg caaatataag
    12481 gatggctatg acaggaataa atatacagat tgacaatata acacgatgtt ttttttcctt
    12541 ttggtcataa tgaaagtaat ctgcaagcat taatcttaaa gatcttaaag ctgtatctcc
    12601 agaagttatt ggtaaaacaa ttacacctat tatagctatg ataccaccaa tagaaccaag
    12661 taaatctctt gcaaccaaac ctattacatc aggagaacct actaattcct taggtattcc
    12721 tttattgtaa atacccatag cagctgcagc ccaaatcatt gcaattaaac cttctaaaat
    12781 catcatatta taaaaagtag tttttccttc tttttcattt gaaactgatc ttgctataag
    12841 agtagcttga gttgagtgga atccagaaac aataccacaa gcaactgtaa caaagaatgt
    12901 aggaataaga ggaataccat caggatgaat acctttccaa tttgcaagac ttaaattagc
    12961 taaatcataa ccttgtgtaa agattccaat tccaacacca gcagcagata aaagtagaat
    13021 agctccaaat attggatata cctttcctat gattttatca attggaaata gtgttgcaca
    13081 caagtaatat agaaagataa gtccataaac tatccagaca actggattat ttatagtcga
    13141 tttcatattt aaaatttgag taacaactaa atccccaggt gtataaataa aaactgcacc
    13201 aactaaaagc attaaaagac aaaggaatat attatagact tgaaatactt tattacctaa
    13261 aaatctactg ataagagaag gcatttgtgc tcctttttct cttatactaa tcattccact
    13321 catatagtca tgcaaagctc ctccaaatac acagccaata ggtattagta tgaatgctat
    13381 tggaccaaac aatatacctt ggataggtcc tagaattggt cctgtaccag ctatattaag
    13441 caactcaatt agactatttt tccatttttt cattgggaca tagtctactc catcactttg
    13501 agcaagtgca ggtgtttttc tgtcatctgg gccaaatact ttttcacaat aagcaccgta
    13561 taaagcacct cctattagta atatagccaa gccgattaag aatgttgtca taatatcacc
    13621 ccatttataa aattttttgg tctttgtata catattatct aaatgtgtag atttatattg
    13681 aatttaattc tggacaacct acaaattcta taaagtatta caactaatat aatttcagaa
    13741 ataaatacat atacataacc atgtacttag tatacaggta tttggtaata ttttcaatgt
    13801 tatagcaata aagaatataa acaaaattct gataatattt tacaaaaaaa ttaaaaaata
    13861 taaaaattag tttaaataga tggagaaaaa aggaaaatca attgagaata taaatgtcta
    13921 tatcttaaca atattaagag gtttaaaatt cttgaagaaa gaatagtcaa gtgatataat
    13981 gggtttacag gtgtatatac actagtacat atagaaagcg gtgcaattat ggataaagat
    14041 ttaacatatc aaatcaaaga gctaaagaaa gaaaaaaatg caataatact tgcccatttt
    14101 tatcaaccac cagaaataca agaattagca gatgcagttg gtgactcata ttatttaagt
    14161 gagattgcta gagattgtaa agaagaagtg gttgtatttt gtggagtaag gtttatggga
    14221 gaaagtgcta agatattatc acctgaaaaa actgtattga tgccagtaag taacgctggg
    14281 tgtgctatgg cagatatggt agatgaagaa ggcgtgataa aattaaaaca acagtatcca
    14341 aatgctttgg ttgtatgtta tataaactct acagctaaag ttaaggcaca ttgtgatgtt
    14401 tctgttacat cttctagtgc aattaaaata ctagaaaaca tagataataa agaaattata
    14461 ttccttccag ataaaaattt aggaggatat attgcagaac aatttccaga taaaaacttt
    14521 atattttggg acggatattg taaataccat aacaatataa gagctgaaga aataattgag
    14581 ttaaaagata agtataaaaa tgcagaagta ttagtacatc cagaatgtaa aaaagaaata
    14641 agagatttag gagattatgt aggtagtaca tcaggaataa taaaatatgc tactaatagt
    14701 aaaaacaaag actttataat agcaactgaa gaaggtatat tacatgaatt aaagaaaaat
    14761 aatcctaata aaaactttta tattcctggt ggaaaaatac tatgtacaga tatgaaaaag
    14821 acaacattag aaaatttata tagtacactt aaaaatatgg aaaatgaagt tattatagaa
    14881 gatgaaatca tggaaaaggc tttaaattca ctgcttaaca tgcacaaatt agcagaagga
    14941 tagagggata gacttatgaa tttagaacaa gatgttttaa tagttggaag tggagtttca
    15001 ggattatact gtgcattaaa tttagataaa agtttaaatg tgctagtagt atcgaaatca
    15061 acaattgaaa acaacaatac atatcttgca caaggtggaa tttcaactgc tagaaatatt
    15121 gatgatatag aatcctttgt agaagataca atgaaggctg ggcagtataa aaatagagtt
    15181 gaagcagttc aagtacttgc agatgaatct atagaaaatg tagggcagat agtagaatat
    15241 ggtatgcctt tagacaaaga aaatggagag atagattaca caagagaagg agcacatagt
    15301 gtaaatcgaa tagttcatag taaggataat actggagaag ttgtttttaa aactttatta
    15361 aaagaagcta aaactagaga aaatataact cttatagaag atgcctatct tttagatata
    15421 ttaaaagatg gcaataaatg tataggagct agaattttta aatctaaaaa agagattcat
    15481 gttttttcaa aaatagtagt tttagctaca ggtggaattg gtgggctttt taaaaattct
    15541 actaatcaaa gacatcttac aggtgatgga attgcaatag ctttaagaaa taatataaaa
    15601 atagaaaatt tagaatatat tcaaatacat cctacagcct tttatgaaga aaataatgaa
    15661 ggtaggagaa tgcttatatc tgaatcatta agaggagagg gagctaaact tttaaataaa
    15721 aataaagaaa gatttgtgga tgaacttctt cctagagatg tagtttcaaa agctattttt
    15781 gagcaaatgg aaaaagataa attaccttat gtgtatttag atgcaacaca tttagatagt
    15841 gaatatttaa ttaatagatt ttcttttatt tataatgaat gtttagctag aggaacagac
    15901 ataacgaaag aatgtataaa ggtatctcct gcacaacatt attttatggg cgggatacat
    15961 gtagatttgg attcaaaaac atctatggaa aatttatatg cagttggtga aattagttgt
    16021 acaggtgttc atggagccaa tagattggca agtaattcat tacttgaagg gcttgtattt
    16081 tcaaaaagag cagctaaaaa tataaactca gttatagata atgtaaaagt taagtttata
    16141 gatgtaccag atatggatat agatatagaa caggtaaaaa aagaaaataa aatattagtc
    16201 ataaaagaaa ttgaaagaac aagtgaggat tttggcgatg aattatttga ttattgatag
    16261 aatgatacag gatgctttga tagaagatgt tccaagtgaa gatataacaa ctaattctat
    16321 tgtggatgaa aattctaaat ctactgtaga tttaatttgt aaacaagatg gtataatagc
    16381 tggcttaggt gtatttaaga gagtttttga gatacttggt gatgtagatg taaaattata
    16441 taaaaatgat ggtgatagag taaagaatcg tgaaaagata gctgtattaa caggtagtac
    16501 tagaaactta cttgtaggag agcgtgtagc attgaattat ttacagagaa tgagtggtat
    16561 agctactatt acaagtaggt atgttgaaaa gcttgaaggt acaaatacaa aattattaga
    16621 tagtagaaaa acgataccta atcttagaat attagataag tattctgtta aagtaggtgg
    16681 aggatgcaat catagattta atttatctga tggaatattg cttaaagaca accatattgg
    16741 ggctgctggt agtgttaaga aggcagttga attagctaga aaaaatactt cttttgttag
    16801 aaagatagaa gtggaagtgg aaactttaga catggtaaaa gaagcaatag aagctaatgc
    16861 agatataata atgttggata atatgagttt ggaaatggca aaacaagcag tagatattat
    16921 aaatggaaga gccattgtag aattttccgg aaatgtaaat ttagatacta tagaagatat
    16981 aggaaaaatt ggtgtggatg ttgtttctgt aggagcgctt acacattctg ttaaagctct
    17041 cgatataagt atgaagaatt tgagaaatat ataaatttta tattgaaata aacttaatta
    17101 aaatagactc cttaaatttg ttttataaat ttaaggagtc tattttttat tcattaaaat
    17161 taataaaaat agaaaaaaat caatcaataa tttaataaaa atagataaat actatataca
    17221 cttttaataa tatagttaat atctatttta cagataaata agaataaact tataatataa
    17281 ttgtatctgt aaaattttgt aaaatataaa aagtataggg ggaataaaaa tgaaagtagc
    17341 ttatattttt agtagtacta atacacataa aattctagac aaaatgataa taccacaact
    17401 tgaacaagga actcatggag tagacgttct agggatgttt ttctttatgg ataatacctt
    17461 atttttatct aaaggtaatc ctgtaggcga gagactaagt aaattacatg aaaaaactga
    17521 tatgataata atggcttgtg accaatgtgc tatagaaaga ggtatagaga ataatttggt
    17581 tgatggagct acaattggat gtttcccaaa cctatatgct tgtttaggca gtgcaggtgt
    17641 ggaccaagtt ataacgttat aaattagtat aggatacaat ccatagaaaa atttattttc
    17701 tgtggattat tttaagtaaa agataaattg tttaaagtag gggatgaaca aatgtctaaa
    17761 attttagttg aatttataaa cacttgacca tcttgtgatg aacatggagt gtttataaaa
    17821 aaaatgtcag aaaagtatgc tgataaattg gaaataaaat tatatcaagc agggaaagat
    17881 ttttcgtata taaaaaaata tgggattata acaaaaggaa ctttaataat aaatcagaaa
    17941 aagaaatatg atagattaaa taaagataca attgaaaggg caatagtaga agctataaac
    18001 aataattaaa agtttaaaaa ttaggagtat caaaaatgaa tatattagaa attgttttaa
    18061 atggagttaa agatactata gtagcttcta tagttacact gaatggagca tcaggatggc
    18121 ttatgataag ttttatattg gcaggtgtac ttcacaatgt tttaactcca agaaggtttc
    18181 aaaaacaact tggaaataag aaattttctt ctttaattaa aagtactatc tcaggaatgt
    18241 ttttaccaat atgtagttgt ggagtaatac cacttggaat aagtatgtat tattcaggag
    18301 catatttagg accagtactt gcttttatga catcaactcc aataataaat ccaatagctg
    18361 tagttctttg tctaggtctt ttaggtccta aagttacaat catttatcta atagctggtt
    18421 ttttagtacc atttttagta ggatttttag gaaattgttt tggaaaagaa gaattatcac
    18481 ttcataatga ttcagaggaa gaagtaatag aattagaaga agaaaaaaga agttttatgg
    18541 aaaatatgat tgatggtctt aaatggtctt ttggtgaatt agcccttact attagtaaat
    18601 atgtagtaat gggtatggtt tttgcaggat ttataacaac tatttttcca aatagtataa
    18661 ttcaaaaata tttaggaaat ccaggtttgc tatctcttgg aagtatagca atacttgcat
    18721 gtgttatgta tgtatgtgct gtaggacata taccgtttat agcagccctt gttgcaagtg
    18781 gagcatctcc tggtatagcg ataacatttc taatagctgg agcagctaca aatctaccag
    18841 aacttataag tatgtttaaa cttattggaa aaagaactgt tattatatat tctgtaactt
    18901 tgacactgtc ttcaataata attggatata taacaaattt acttttagtg aataataact
    18961 cagtaaattt agatgcaaca aattcttcaa tacagttggc aaataaacta atgttaaata
    19021 tacctgatag ttttgagtat gtatgtacta ttttaattat tttatttgca tttaaatcaa
    19081 taataccaaa gatgaaagag gtcataggaa atcatgctta aaaagatagt tataatatcc
    19141 tgtatagtag ttgtgttaat cattttatca aaaatagttg atgataacat caaagaagat
    19201 gcttctattc caaatgtgaa taaagaaaca ttagaatatt ttagaaaaaa ttataaagaa
    19261 gacataataa cttgtgctga agaagactta aataatgatg gaaaaaaaga tttagtagtt
    19321 atatataaaa aaagcaataa ttcaaatgaa atggtagtcg tagtttctga taaaaatagc
    19381 cactatataa caaagccaat accagcacct atagagaatc aaactataac ctttaaaaat
    19441 attgatgata aagcaccaat agaagtaatt gtatcagggt ctaaaaatgg taatgtagga
    19501 tatgctattt acagagtaga gggtaaaaaa tttgttgatt tatttggcga agatatggac
    19561 aaatgttgct aaaaataaaa ataatacata aaggtgggaa atttatatga aattatctaa
    19621 gaagattcaa gcattaatct tatttggtgt aatgggtaca tctatattaa caggatgtag
    19681 tagtaaacca aaagaaaaag aagaagcaaa aactggaaaa gatgattata caataacagt
    19741 aggttattat aattgtgacc atatgactgc gggaccagta gcagaatcgg caggaatata
    19801 taaagattta ggtcttaatg taaaaactgt tggaaatggt aaagttcctg aagctatggc
    19861 agcaggaaaa atggatgcag gttacatagg aactaagggg ttagttggag caatacctaa
    19921 gggttcacca ataacaatag cagctaacaa ccatactggt ggttctgaat atttaatagt
    19981 ttcaaaagat ataaaagaac ctaaagattt aataggtaaa aaaattgcaa cagatatgag
    20041 tgatttctta tggacaagtg attatggtcc agaaacaggt ctaccaactg acccatcaaa
    20101 atatgaagtt gtaaatatgg attcagacaa agataaatat ttagctctta aaactgggaa
    20161 aattcaagct tttacatctt gtgacccttg gggttctgta gctgaaaatg acggtgcagg
    20221 aaaaataata gcatctactc aatataaaga aaaagctaat ggcaaagaat ataactgttg
    20281 ttcattctca ttaaacaaaa actttataaa agaacatcca gatttagcaa aaaaattggt
    20341 tctagcacat acaaaagcaa tagaatatat atatacaaat ccagctgaag cagctaaaat
    20401 atttgcaaaa tattataatg tagaagaaga agttgcactt agaactatct ataagaagac
    20461 tgttggagaa ggtagaactc ttacttggaa agttacaggt gaagaatata aaaataattt
    20521 acaaatgtat aaagatttaa aggctttaga tgatatacct aaatatgaag atacgattga
    20581 tacaagctta ttggactctt gtggagctga tgattttgac aaatttataa aagaaaaagt
    20641 taacccagag tttcctgaag gtatgagtta tgaagactgg aaagcaaagg tattataaat
    20701 aaatggagaa tatgaaaaat ataataatac tttttatcat tattttggga agtatattta
    20761 taatattttt taattttgaa gataaagaag aaataaaagg aaaattaaga ataggggtat
    20821 ctgatgatac ctctggtttt gtaataaatt atatggtaaa tagagactac tttgaaaata
    20881 taaaagtaag tgatataatg gatattttta caattaatga ttgctgagca agtacaatgc
    20941 agtgggctct gagttcagag gaaatagata tagcaatcat atgtaaagat gctgcaaaac
    21001 aatatgtaaa tgtaaatagt ggatttgaaa ttgttggtac tgtagttcaa aattctgata
    21061 tatttctatt aagtgataat aatcctgaaa agataggagt cattcaaaat agagattacc
    21121 agtcagaatt ggttaaaaaa tactacaaga atgtggaagt agctccctta ttagggactg
    21181 cactagcata tggattagaa agtaatttag tagatggtgt tgtaatagat tgcataaagt
    21241 caattgggtt agagggcaaa agaaaatcta caactaaatt aggagattat gatacctatg
    21301 tattagttgt aagtaaagag tttaaaaaaa gtaaagcata tagtgatttt ataacacttt
    21361 acaataaaag tgttgatgct ttaaagaata aagctacatt taagaaagca ttaaaagagt
    21421 acaggaatat aagaaatata gatatggagg aaataagcaa ttggaaatta aaatttctaa
    21481 agataaaagc gatagattaa ctaagaaaaa aactgatggt agtatggata ttttatataa
    21541 aataatacta ttagttatta tatttttagt ctggcatttt gcagcaaaag atatagggag
    21601 ttctttgtta cttccaatgc cagtagatgt aataaaaggt ttctttttct gtgtgacaga
    21661 tgcagagaca gtcactaatc tttttataac attgcagaga gtacttaaag gttttatgta
    21721 tgcattattg tttgggcttc caataggatt tattatggga ttttctaaaa cttttgaaag
    21781 ggttttatct ccagtagtag attctgtaag gcaagttcca ataatggcat gggttcctct
    21841 tactatagta tggtttggta taggagatgg tccaacaata tttttaatag ctttttcagg
    21901 ggttttccct ataattttaa atactattca aggagtaaga gcaatatcaa aggattatta
    21961 taatgcagct agaagtatgg gagcatcgcc aattgtgata tttacaaatg taattgttcc
    22021 cgcatctctt ccagatatac taactggttc aaggattgct ataagtacag gatggatgtc
    22081 tgttatctga gccgagttta tagctacgag tgccggtctc ggttactcaa tggtacaagc
    22141 tcaaacatta atgcatacag atgtattaat tgggcttatg atatttgcag ctttaatagg
    22201 atttataata gatagggtat taaagtttat aaataaaatc ctttgtagat ggaggtttgc
    22261 agattaatga agttaagtgt aagagatata aataagacat ttgtaaataa tagagtacat
    22321 acaaaggttt tagaagatat aagtatggat atagatgatg gacaatttgt ctgtctttta
    22381 ggaccatcag gttgtggaaa aacaacttta cttactataa taggtgggtt tcaaaaatca
    22441 gaacgtggag atgtttttat aaatgataaa agagttaaaa aaccgggcat agatagagct
    22501 tttatatttc aaaattatgc tttatttcct tggaaaacta taagaggtaa tgttctattt
    22561 cctatgaaac agcagaaaat accaaaagaa aaaagagaag aaatgctaga agaacttttg
    22621 gttatgtcag acttgaaagg aaaagaaaaa ctgtttccac atcaattatc tggaggaatg
    22681 aaacaaagag tggccatgat aagagcttta gcatgtaatc cagaggtatt actaatggat
    22741 gaaccattag gagctgtaga ttttcaaatg agacaaaatt tacaggaaga gctagaacga
    22801 atatggataa agaagaaaat aactgcatta atggtaactc atgatgttga tgaagctgta
    22861 tatatgagtg atagagttat agtaatgtcg agagataaag gaagaattat tgatgatata
    22921 aatatagata ttccaagacc tagaattaga ggaagccaaa aatatgaaga gtacaaaaac
    22981 aaattaactg atactttatc aaagtgttat gaagtttaaa gaggagtaga catgagtcaa
    23041 atagaaaaat acaatagttt ggataataca caacagaaaa taagaatctc tattatggat
    23101 atgataatag ataaaggttc tagtgttaca cttcaagaag tgactgaata tataagtaaa
    23161 aagttgaata tagaaaaaga atatatagaa aggacactac aatattttat ttataaaaat
    23221 ataatggttg ttgatggaaa tagtataaat tttatatatc ctgtatcagc attgccaaca
    23281 aatcataaag taactttgag agataataga agtttcagtg caatgtgtgc tatagatgct
    23341 ataggaacct cttgcacatt caaccaagat ataaaaataa actctatttg tagtgctaca
    23401 ggtaaggaaa tagaagtaat tataaaaaat gaaaagattg agtatgtgaa taatccaaac
    23461 ttaagagtgc ttcatataaa tcttgataag catctaaatt gggctgctag ttgctgaaat
    23521 ataatgaatt tcttctggac gaagagtgat tttgatgctt ggacaaacga agcgggatta
    23581 tctgatgatg aagatatata ttgtttagat attaatgagg caatagtaga atcatataaa
    23641 atatttaaac tcaaacaaaa ggtactatca tagatataca tatatgaaaa actaaaaatg
    23701 acctttagat aaaaatgata ggtaatatcc atactatata aattagtata gagtagggat
    23761 gtgaaaagta tttaaatcta aaggtatatt tttagttttt tttagatgat tagatagcct
    23821 tattaagtat atatttattt ataacctttg caactccatt ttcattatta gtatcagtaa
    23881 cataatctgc aatttccttt atactattac gtgcattccc catagccaca ccaagacctg
    23941 catactttat catatgttgg tcattttctt catcgccaac agcaatagca ttttctatag
    24001 gtatatttag attcgtacat aatgttttaa gagcagttcc tttattagta gttttaccta
    24061 aaaattctag ataaaaagga gcagacctta ctatagtata tttattgaat aaatttttag
    24121 gtagttgctg tatgatttct tccaatcttt ctggttcatc aactatcata attttacaga
    24181 ttttaataga ttcatcaatt ttatcttttt gtatatattt gacttctata ttatttaatt
    24241 tcgcttcatg aagtgtatat ttgccaggcg tttcatttgg tgtaattaaa tttgtcattg
    24301 tatttatgtg tattttagtc ttatattttt tacaaacgtt attatatata aaatcaaaat
    24361 catctaaact catttctaca tcatgtatga ttttctcact tttaacttct tgaataattg
    24421 ctccattaaa gcatataaca taatcacctt catctgttaa atttagttga tttagaagtt
    24481 cttttacacc aggaagaggt ctgcctgttg ttataactac ttttgcccca gccctttttg
    24541 cctcctgaat ggactcaaag acttcaggag taatcctttt ttgtgtattt aatattgttc
    24601 catcaatgtc tagtgcgatt aatttgtaca tatcaacctc caataaatta tatactcaaa
    24661 tttcctgtga aaacgttgac acagctagtg ttgaacaaag tttcagtggg aaatatggtt
    24721 tgttttgtat actattatat caagagataa gtaatttaga aagtttaata atattaattt
    24781 tttgataaaa atattactta tgtgagatga gaatgtaaaa actttgtaat caataattcc
    24841 aaaattctta attacaaata atggtatcgt ttgcattgtt aatataaata aatgtatatt
    24901 atatagttag aagtttgaat aaatagactg atggtaattt gttatattag ttagttttgt
    24961 gttataaaat attgtaattc cattaatcat atgttattat tcaacatgat tataatataa
    25021 agaatatttg atatatataa taatttataa aagagtatta catagtacat gtaaatctat
    25081 atttataaat acaataaagt aaagttgtaa aggagtgctt taaatgaagt tttcagagtt
    25141 taagtatgaa agacccaatt ataatagtat gaaaaaagag tttttgagtt gtgtagaaga
    25201 tataaataat tctcgtaatt ataaagagca acaaaaaaat atacataaga ttaatttatt
    25261 aagaaataaa atagaaacgt tgtcaaatat agcttcgata agatatagta cagacacatt
    25321 taataaattc tacaaagaag aaaaaaatta ttgggatgaa tatatgcctt tgtatgaaga
    25381 attaaattca tatttttata atgccattgt aaattcaaaa tttaaatatg atttgataaa
    25441 agagtttgga gagcaatttt tcacaatagt agagtattct ttaaaaagtt tttccaaaga
    25501 aataataagt gaattgcagg aagaaaataa attgtgttca gagtatacta gacttttagc
    25561 ttcagctgag ataatgtttg atgggaaaat aagaaattta tctggtatgg ggaaattcat
    25621 gtattctaag aatagaaaaa caagagaatt agcaaacaaa gcatattata atttctttga
    25681 agaaaatgaa actaaatttg atgacatatt tgataaattg gttaaactta gagataagat
    25741 gtctaaaaaa ttaggttttg aagattttgt agagcttggg tatgttagaa tgatgagaag
    25801 tgactacaga gaatatatga taaaaaatgt tagaaaacaa gtactaaaat atgttgttcc
    25861 aatggcaaat gagctttatg aaaaacaagc taaaagaatt ggtctagaat atttaagtta
    25921 catagatgaa ggcgtggaat ttttaacagg aaatgcaagt ttaaagggag attcaagata
    25981 cataattaaa aatggtaaaa ggatgtattc tgaactgtca aaagaaacaa atgaattttt
    26041 tgattttatg ttagaaaatg aacttatgga tttagaaacg aagaaaggaa aaggagctgg
    26101 agggtattgt acttatatac cagattataa gtcaccattc attttttcta attttaatca
    26161 aactgctgat gatatagatg tattaacaca tgaggcagga catgcatttc agttgtatat
    26221 gtctagatgg attgatatgc cagaaattaa ttttccaacg cttgatagtt gtgaaatcca
    26281 ttcaatgagt atggaattta taacatggcc gtggatggat ttatttttta aagaagatac
    26341 agataagtac aaattcactc acttatcatc ttcaattaaa tttatacctt atggagtaat
    26401 tgttgatgaa tttcaacact atatttacaa aaatcctaat gtagataagt caaaacgaaa
    26461 agaaatctgg agatttttag agaaaaaata cttacctcat agaaaatatg gtgataattc
    26521 ttttttagag agaggttgtt ggtggtttaa acaaggacat atatttaaaa atccgtttta
    26581 ttatatagat tatgtattag ctcaaatttg tgcgttacaa ttttggaaaa aaatgattca
    26641 agatagagat gcaggatgga aagattatat aaatatctgt aaagttggag gaacaaaatc
    26701 atttttagat attgtgagta tgggaaattt gtattctcca tttgatgatg gctgtattga
    26761 gtctattata ggtgatgtta aatcttggtt tgatgaaata aatgacagca aattataaaa
    26821 aattatgaga attttattgt ataaaagtga aaaattaaaa aatatataaa aagtgattat
    26881 ttgttgtgaa aatagtcttg tttttaacta aattgataaa ataaactaaa aaagatttgt
    26941 acttaaaaaa aaaagaaaat acaagaaata tgcaatatca acatttggaa aaaaaagtaa
    27001 aaatgtatac aaaatacata taaaaaaact attgcaaatt ttatagaatg tttatatact
    27061 taagtcaaca gagatagaaa aaagctattt atagaaaaca aaggataaat tgcttaattt
    27121 taaattttct gaaaaaaata ataatccgga tgaagagtat gggagactgc tttataaaaa
    27181 gcgaccgaag ggacaagttt aatcttactt acccaaaaga ttagatgaaa ttctcaggta
    27241 aaggtgactg tacttggacg gggctctgaa aatgatttta acagggaaga caaattggga
    27301 gatttgtttt tcctgttttt tatttatcaa gtaataaata aaagtttgaa agagagtaaa
    27361 aaaatgataa taattacgaa taatccaaaa gtaaaagaag aagttcaagg cagagaagtt
    27421 ttgtttaaag acacaactta tattggaatc ttagaagcaa gtagagattt gatacatgaa
    27481 ggttatgaac ttttatctca tccactctat gggagtgtaa agccaaatga aacaccttac
    27541 aggactgtta tactaaagaa aggaaatcgt ttggatataa actctttaac cttaattgaa
    27601 gaagcaatca tcactgcaag taaatttcag aataataaaa agactccaaa atggacagaa
    27661 agtgttcaag atgattttag agtaattgat tatgatatat tttataatac aatacaaaga
    27721 atgcagtatg aataaaaaaa tttgttaaaa agttattaaa acagcctaaa tattaaatct
    27781 aatttaatat taaataaaat gagaaattta attggggagg ataagtcaaa atggaaaatg
    27841 tgtatgactt agtaataatt ggttcaggac cagcagggct tgcagcaggt ctttatggag
    27901 caagagcaaa gttaaaaact ctaatcctag aaaaagacaa gactggtgga caaattgtaa
    27961 taacacatga aattgcaaat tatccagggt cagttccaaa tgcaacagga ccaagtttaa
    28021 tagctagaat ggttgaacag tgtaaagagt ttggagcaga aatgctaaga gacaacattg
    28081 ttgatactga gctagatgga gatataaaag ttttaaaagg ggaaaaggca gaatatagag
    28141 caaaagcagt aataatagga acaggagcaa caccaagaaa gattggttgt cctggagaaa
    28201 aagaacttac tggtaaaggg gtttcatatt gtgcaacttg tgatgcagat ttctttgaag
    28261 attttgaagt atttgttgta ggtggaggag acagtgcatt agaagaagca atgtatttaa
    28321 ctaaatttgc tagaaaagta acaattgtac atagaagaca aggatttaga tgtgctaaga
    28381 gtgttgaaga aaaagcaaaa gctaatccaa agatagagtt tttattagat acagtaattg
    28441 aagaaatcaa aggtgatgga atattagaat cagttgtatt taaaaataaa gttactggag
    28501 aaactcatga atactttgca gatgaagaag atggaacaat gggagtattt gtgttcgttg
    28561 gattagatgc tcaaactgat ttattcaaag gtaaagttga tatggatgaa aaaggttaca
    28621 taataactga tgaagatatg agaactaaca taccaggcgt atttgcagca ggagattgta
    28681 gatctaagac tctaagacag gttgtaactg caactaatga tggagcaatt gcatctatag
    28741 tagctgagaa atacattgat gaaaaatttg ggaactagaa tctgtaaatt aagaaatagt
    28801 gtctgtaaat ttaatgaata atatttaaaa ttaaaattaa tatttaaaat taaaataaaa
    28861 atttcaaggg ggaataagaa aatgttagat ttagataagg ctacatttga agaagaagtt
    28921 ttaaatgcag aaggttttgt atttgttgat ttttggagtg aaggttgtga gccttgtaaa
    28981 gctttaatgc cagatgttca taaattagct gagacttatg gggataaaat aaaattctgt
    29041 aagatggata caacaaaagc tagaagatta gctataaaac aaaaagtatt aggtcttcca
    29101 actatggcta tatacaaaga tggagaaaaa gtagacgaag ttactaagga tgatgctaca
    29161 gttcctaata tagaaaacat gattaaaaaa tatctttaat atgttgataa agtattaaca
    29221 ttaaaagaaa aaatctattt ttatctgaag ataagaagaa aaaggttttt atgtccaact
    29281 ttgttaaaaa aataatttta aataaaataa tttttttaca ataaatattt tttagtaata
    29341 aaataaagat ttaaggcata aaaacacaat aatatatctt taaaaaaata agataaattg
    29401 taaaatgata taattttgag aaaaactcaa atgcatatac acaaattaaa taacaggagg
    29461 tgcaagtatg cgtcttgaat tagggaaaat ctttataaaa gatattcaat ttggtgatgt
    29521 aacagaagta aaagatggag tattattcat caataagcaa gagatgctac aagaaattgg
    29581 tggagatgag catattaaat ctatagatat agaattagct cgtccaggtg aaagcgtaag
    29641 aataacacct gtaaaagatg ttattgagcc aagagtaaaa gttgaaggta atgggggaat
    29701 cttccctggt atcatgtcta aagttgacac tgtaggagaa ggaaaaactc atgctttaaa
    29761 aggtgtagct gttgtaacta ctggaaaaat agtaggtttc caagaaggta taatagacat
    29821 gactggagaa ggagctaagt atactccatt ttctaaatta aataatgtag tagtaatagc
    29881 tgaacctatt gatggtttaa aacaatacgc tcatgaaaaa gctgttagaa tgataggatt
    29941 caaagcagca atgtacttag gagaagtagc aagaaatctt actccagatg aagtttcagt
    30001 atatgaaact aaacctcttc ttgaatcaat aaaagaatat ccagagcttc caaaagtagg
    30061 atatgtttac atgcttcaga ctcaaggttt attacatgat acttatgtat atggagtaga
    30121 tgcaaaacaa atagtaccaa ctttattata tccaacagaa ttaatggacg gagcaattgt
    30181 aagtggtaac tgtgtttctg catgtgacaa aaacccaagt tatgttcata taaacaatgg
    30241 ggttgttgaa gacttatatg caagacatgg taaagatata aactttgtag gtgtaattat
    30301 aactaatgaa aatgtttatc tagcagataa agaaagatct tcaaactgga cttctaaatt
    30361 atgtaaatat ttaggattag atgcagttat agtttcacaa gaaggttttg gaaatcctga
    30421 tactgacctt ataatgaact gtaagaagat agaaatgcaa ggtgttaaaa cagttatagt
    30481 tacagatgaa tatgctggac gtgatggagg ttctcaatca cttgcagatg cagatgtaag
    30541 agctgatgct gttgtaacag gtggaaatgc caaccaagtt gtagttcttc ctaatttaga
    30601 taaagttatt ggacatttag aagtagtaga tgtaatagct ggtggttctg atggaagttt
    30661 aagagctgat ggaactattg aagttgaaat ccaagctata acaggtgcaa ctaatgaaac
    30721 aggatttggt catttaactg ctaaaggata ttaagaagta ttttattaaa cgattgagta
    30781 atgttaataa aacatattat agataattat aataagtgtt atagatatat aaaaatatat
    30841 tagaaatata ataaggagga ttattatgag tttacttagt aataaaaagg ttcttataat
    30901 aggtgaccgt gatggtatac caggacctgc gatagaagaa tgtgtaaaaa cagtagaagg
    30961 agcagaggtt gttttctcat ctacagaatg ctttgtctga acagctgctg gggctatgga
    31021 cttagaaaat caaaacagag ttaaagatgc tgctgataaa ttcggagctg aaaatgttgt
    31081 gattttacta ggtgctgctg aagccgaagc tgcaggtctt gcagccgaaa cagtaactgc
    31141 tggagatcca actttcgctg gaccacttgc tggagttgcc ttaggattaa gtgtttacca
    31201 cgttgttgag gaaccaataa aatcattatt tgatgaaagt gtatatgaag accaaataag
    31261 tatgatggaa atggttttag aagttgaaga aatagaagaa gaaatgtctg gtataagaga
    31321 agaattttgt aaattttaat gcctaaaagc aaaaggggtg aaactaagag atgggaaaac
    31381 taaaagcagt acattatatt aatcagttct ttgctggtat aggtggagaa gaaaaagctg
    31441 atactaagcc acatgtggca gaaactttac ctccaataag tttacaactt gataaattat
    31501 taggtgaaga tattgaaata gttggaacag tagtttgtgg agatagctat ttcaatgaga
    31561 atatagatag tgcaagtgaa gaagttttat caatggtaaa aggttttgaa ccacaacttt
    31621 tcatagctgg tcctgcattt aatgctggta gatatggggt tgctgctgga acaataacta
    31681 aagttgtaaa agatgctttg aatataccag ctttaacagg tatgtatgtt gaaaatccag
    31741 gagcagatat gttcaaaaag gatgtttatg tagtagaaac ttctgattct gcagcaggta
    31801 tgagaaaggc acttcctaag atagctaaac ttgcagtaaa acttgctaat ggagaagaaa
    31861 ttggaactcc taaagatgaa ggatacatag ctagaggaat aagagttaac tacttccatg
    31921 aagacagagg ttctaagaga gctgttgaca tgttagttaa gaaaataaaa ggtgaaccat
    31981 ttgaaactga atacccaatg ccaaactttg atagagttga cccaagtaaa gctgtaaaag
    32041 atttatcaaa atgtaaaata gctttagtta cttctggtgg tatagttcca aaaggaaatc
    32101 cagatagaat agagtcttct tcagcttcta aatatggaac ttattctata gcaggagtta
    32161 tggatttaac agaagagact tatgaaactg cacatggtgg atatgaccca gtgtatgcaa
    32221 atttagatgc agatagagta ttacctgttg atgttcttag agatttagaa aaagaaggtg
    32281 taattggaaa attacatgag actttctata caactgtagg taatggtaca tctgttgcaa
    32341 actctaaaaa atatgcttct gaaattggag cagcattagt ggctgatggt gtagatgcag
    32401 taatattaac ttctacatga ggtacttgta ctcgttgcgg tgcaacgatg gtaaaagaaa
    32461 tagaaaaaac tggacttcca gtagttcata tgtgtacagt agtacctatt tcattaacag
    32521 taggggctaa tagaatagtt ccaactatag caatacctca tccacttgga aatccagcgc
    32581 ttgaccctac agaagaaaaa gccctaagaa gaggccttgt agaaaaagca ttaaatgctt
    32641 taactacaga agtagatggt caaacagtat ttgaaaaata aaaataacac acacatatat
    32701 tatatagata aaaatataaa cacacactaa aaataaaaaa taactataca caaaaaaata
    32761 atatagattt catgaatgga taaattggag ttattcattc atgaaatctt aaaaaatgcc
    32821 aaaatacata aacaataata gaatcaaata tacaataaaa tacaatgatt agagctttta
    32881 ataagtttaa taagattaaa aaataagtgt tcataaaatt tatggaggtg cacaaaatga
    32941 cttatccagt tttaaaaggg gcaggctatg tacttattca tactccagat atgatagtgc
    33001 aaaatggaag tacttgtaca gttgaaagag caacaaatcc agattcagaa tttttaaaag
    33061 aagtaagtaa tcatataaga agttatgaag atgtagttaa ctacatgcca aatcaagttt
    33121 atataggaaa tagaagacca gaagaattaa gagatttacc aatgccatgg tgtgaacaaa
    33181 agattgaggg aactagaaat ggtaagtttg gagaaataat gcctcaagat gagttcatag
    33241 ctttaatgca aataagtgat gcatttgatt tagtaaaatt atcacaagaa tttattgatg
    33301 aagtaaagcc aaaaatcgaa aataactacc ctgaaattgc accatttgta ggtaaattaa
    33361 agggtgatga tatagaagaa ggtaaagaat tagtagcaac acacatagct gaaggattat
    33421 accatgatgg aaaatttgtt ggttatgtaa aaagagcaca tgatgtagat gttaacttaa
    33481 atgcccatac aatgtttgaa aatctagtag taaaagcatc tggagtttta tctgctattc
    33541 aaatgctaag acatagcaaa atagaccctg ctgaaataga ttatgttatt gagtgttcag
    33601 aagaagcttg cggagatata aaccaaagag gtggaggaaa ctttgctaaa tcaatagctg
    33661 agatagcagg acttcaaaat gcaactggtt ctgacacaag aggattttgt gcagcaccaa
    33721 ctcatgcatt aattcaagca gcagctttag taaaagctgg tatacataaa aatgtaatgg
    33781 tagtagcagg tggagcaagt gctaaacttg gaatgaatgc taaagaccat gttaaaaaag
    33841 gtcttcctgt acttgaagat gttgttggag gatttgcagt acttgtatct gaaaatgatg
    33901 gtgtaaatcc agttataaga actgatttaa caggtaagca tactgttgga actggttctt
    33961 caccacaagc agttatgaca gcacttataa cttcaggtct tgatagagct aatttaaaaa
    34021 taacagatgt tgatgtgtat tcagttgaaa tgcaaaaccc agatataact aagccagcag
    34081 gagcaggaga tgttccagaa gcaaactaca aaatgatagg ggcacttgct gttaaacgtg
    34141 gagacttaga aaagaaagaa ttgaaagatt ttgtttctaa caaaggatta ccaggatggg
    34201 caccaactca aggacatata ccatcaggag ctccttatat aggattttta atagatgatt
    34261 taactacagg aaatagaaat agagctatga tagttggaaa aggtagttta ttcttaggaa
    34321 gaatgactaa cttatttgat ggagtatctt ttatagctga aagaaataca ggtgttactg
    34381 aagagacttc aggaatatct aaggatgaaa ttaagaagat aatagctgaa tctatgaaaa
    34441 aactagcatt agatatgtta gaagaatagg ggtgaggata tgtcaaaaaa agtaatcgca
    34501 gatgtattct tagaagtagc taatgcaata gaaagcggcg aatttggcaa aaaagtaaaa
    34561 attggtgtta ctacacttgg aagtgaacat ggtgtagaaa acatggttaa tggtgcacaa
    34621 ttagcaaaat ctaatttatt tgatattgtg cttattggac caaaagtgga aactgacctt
    34681 gaagtagttg aagttaatga tgaaaaagaa atgcatgcaa aaatggaaga attattagat
    34741 tcaggatata ttgatgcttg tgttactatg cattataatt ttccaatagg tgtatctaca
    34801 gtaggtagag taataactcc agcaaaagga aaagaaatga tacttgcaac aacaacagga
    34861 acaagtgcta ctaatagaat agaggcgatg gtaagaaatg ccatttatgg tattgctaca
    34921 gctaaatcaa tgggtaacaa atgcccaaaa gttggaatac ttaatgttga tggagcaaga
    34981 caagttgaga agtgcttaaa agaattaaaa gataatggat atgacatgga gtttgcggat
    35041 tcaattagag cagatggtgg atgtgtaatg agaggtaatg atttacttgt aggtgcacct
    35101 gatgttatgg ttactgatac attatctgga aatatattta tgaaagtatt ttcttcatat
    35161 actacaggtg gagattatga agctcaaggc tttggatacg gacctggagt tggagaagac
    35221 tatgatagaa aggtacttat agtatcaaga gcatcaggtt ctccggtagt agcaaatgct
    35281 ttaaaatacg cttatgatgt tgtaaaggga gatataagta atgttgcaag aaatgaattt
    35341 gcaaaggtta aaaaagcaaa atttgatgac attatatcct ctcttactaa aaaagaagtt
    35401 aaagctgaaa aagtagaagt taaaatgcca gataaggaaa ttgttactcg tcaaatagct
    35461 ggtgttgata taatggattt agaagatgca gtatctgagt tatggaaaaa cggaatctat
    35521 gctgaaagtg gtatgggttg tacaggtcca atagttttag taaatgaagc taaaggagac
    35581 ttagcagttg aaattttagt taaagctgga tatactgcaa aataagtaca ttttttatat
    35641 gtaaataaga acatatagtt ataattttat tgattataat tatatgttct ttttatttta
    35701 tatcttgtca atatttatat attaacacaa tccatttatt tgattctcta gctaatttat
    35761 tatatttatt taattaaaat taagctttct aactaaataa aaactaatga agtattaaaa
    35821 aaaatgaaat taataaaata tgaaaaaatt ttaggaataa agtatctgtt ag
//
LOCUS       DAEORZ010000028        35231 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEORZ010000028 DAEORZ010000000
VERSION     DAEORZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.028"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(121..894)
                     /locus_tag="KRH39_001233"
     CDS             complement(121..894)
                     /locus_tag="KRH39_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBG8511152.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="KRH39_001234"
     CDS             complement(894..1280)
                     /locus_tag="KRH39_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8511153.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFYIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="KRH39_001235"
     CDS             complement(1305..1622)
                     /locus_tag="KRH39_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBG8511154.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="KRH39_001236"
     CDS             complement(1634..1894)
                     /locus_tag="KRH39_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBG8511155.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="KRH39_001237"
     CDS             complement(1894..2190)
                     /locus_tag="KRH39_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG8511156.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KRH39_001238"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KRH39_001238"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBG8511157.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KRH39_001239"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KRH39_001239"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBG8511158.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KRH39_001240"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KRH39_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBG8511159.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="KRH39_001241"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="KRH39_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBG8511160.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="KRH39_001242"
     CDS             complement(8083..9189)
                     /locus_tag="KRH39_001242"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBG8511161.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="KRH39_001243"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="KRH39_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBG8511162.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="KRH39_001244"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="KRH39_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBG8511163.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="KRH39_001245"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="KRH39_001245"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBG8511164.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="KRH39_001246"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="KRH39_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBG8511165.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="KRH39_001247"
     CDS             12283..12990
                     /locus_tag="KRH39_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG8511166.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="KRH39_001248"
     CDS             12959..13588
                     /locus_tag="KRH39_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBG8511167.1"
                     /translation="MRKSLKLIKSKSKEEAISKVIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="KRH39_001249"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="KRH39_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBG8511168.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15177..17069
                     /gene="mnmG"
                     /locus_tag="KRH39_001250"
     CDS             15177..17069
                     /gene="mnmG"
                     /locus_tag="KRH39_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBG8511169.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTNSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="KRH39_001251"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="KRH39_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBG8511170.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17944..18729
                     /gene="noc"
                     /locus_tag="KRH39_001252"
     CDS             17944..18729
                     /gene="noc"
                     /locus_tag="KRH39_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBG8511171.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18864..19637
                     /locus_tag="KRH39_001253"
     CDS             18864..19637
                     /locus_tag="KRH39_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG8511172.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENIDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19639..20502
                     /locus_tag="KRH39_001254"
     CDS             19639..20502
                     /locus_tag="KRH39_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBG8511173.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20535..21677
                     /locus_tag="KRH39_001255"
     CDS             20535..21677
                     /locus_tag="KRH39_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG8511174.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21811..22386)
                     /locus_tag="KRH39_001256"
     CDS             complement(21811..22386)
                     /locus_tag="KRH39_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBG8511175.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22646..23938
                     /locus_tag="KRH39_001257"
     CDS             22646..23938
                     /locus_tag="KRH39_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG8511176.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24043..24645
                     /gene="yedF"
                     /locus_tag="KRH39_001258"
     CDS             24043..24645
                     /gene="yedF"
                     /locus_tag="KRH39_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBG8511177.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24718..24981
                     /locus_tag="KRH39_001259"
     CDS             24718..24981
                     /locus_tag="KRH39_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBG8511178.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24996..25181
                     /locus_tag="KRH39_001260"
     CDS             24996..25181
                     /locus_tag="KRH39_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBG8511179.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25284..26480
                     /locus_tag="KRH39_001261"
     CDS             25284..26480
                     /locus_tag="KRH39_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG8511180.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26694..26972
                     /locus_tag="KRH39_001262"
     CDS             26694..26972
                     /locus_tag="KRH39_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBG8511181.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27009..27443
                     /gene="ssb"
                     /locus_tag="KRH39_001263"
     CDS             27009..27443
                     /gene="ssb"
                     /locus_tag="KRH39_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG8511182.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27462..27689
                     /locus_tag="KRH39_001264"
     CDS             27462..27689
                     /locus_tag="KRH39_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBG8511183.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27832..28152
                     /locus_tag="KRH39_001265"
     CDS             27832..28152
                     /locus_tag="KRH39_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG8511184.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28160..29116
                     /locus_tag="KRH39_001266"
     CDS             28160..29116
                     /locus_tag="KRH39_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBG8511185.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29127..31121
                     /locus_tag="KRH39_001267"
     CDS             29127..31121
                     /locus_tag="KRH39_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBG8511186.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31122..31571
                     /locus_tag="KRH39_001268"
     CDS             31122..31571
                     /locus_tag="KRH39_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBG8511187.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31590..32918
                     /gene="dnaB"
                     /locus_tag="KRH39_001269"
     CDS             31590..32918
                     /gene="dnaB"
                     /locus_tag="KRH39_001269"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBG8511188.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33123..33431
                     /locus_tag="KRH39_001270"
     CDS             33123..33431
                     /locus_tag="KRH39_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511189.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33513..34802
                     /locus_tag="KRH39_001271"
     CDS             33513..34802
                     /locus_tag="KRH39_001271"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBG8511190.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYIAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35134..35209
                     /locus_tag="KRH39_001272"
     tRNA            35134..35209
                     /locus_tag="KRH39_001272"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35167..35169,aa:Lys,seq:ttt)
BASE COUNT        13339 a         4533 c         5572 g        11787 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactttc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatataaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctactttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accgcttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctattgcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcgt gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tgtattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt tcaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtatt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtat ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata atttttgtat cattaattat
     8521 aaaaatagca tttttttcgt taaatccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aaccgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag gatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtatgaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctgagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctattg ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttactag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaagta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaagaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga cttttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac caaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagttat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa atctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa aaagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt ctggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atagtggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaat tcctatacag gagaagatgt tatagaaatt aattgccatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 aggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga cattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataagag atactgatga tattgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaagaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaacttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggc ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac aaatagctta gttgagcttg gtcttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggtg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt acgatttagt gataaaacat cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 ttgagaactt atttagtgca gggcaattta atggaacttc tggatacgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccatttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagagaa agaatttgaa agactaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttgga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaaaggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaagc tagacagaaa ttagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatctgg tgtttctcca gctgatatat cagttttact tatataccta gaacaaatca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgaatgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aaaaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaaaatgaag actataggga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaagtgat ggaaacacca gttaagtacc ctagaaaagc tggtaaacca
    17761 gccaaaaatc ctattaagta atgaaaaatt tattattggt aagcttaatt atttctcagg
    17821 aaataattaa gcttactgta aaaaaacaaa aataaaggaa ttattaaagt agatgcagaa
    17881 ataaatttgt atgtacatat agagaacaaa aataatttat atctaaaata agagaggtat
    17941 gttatggaag ataaaagagt tatggagata cctatagaag atgtagttcc aaatccatat
    18001 caaccaagaa aaatattttc acaagtttca ttggaggaat tgagcaattc tataaaggta
    18061 tatggtataa ttcaacctat aacagttaga gcaaaagatg gcaaatatga actaattgca
    18121 ggagaaagaa gattaagagc agctaaatta gcagagctta aaaccatacc agcaattata
    18181 aataatatga acgatgaatc ttctgctgtt ctagcgttat tagaaaattt gcaacgagaa
    18241 gacttaaatt tcattgaaga agcaattggt tatgaaaact taataaaaga acatgctttt
    18301 actcaacagc aattagcaga aaaattgggc aaaaatcaat ctactgtagc aaataagctt
    18361 agaatattaa agcttccaaa cgatataaaa atgaagctaa tagagaataa tttaacagaa
    18421 agacatgcta gagcatttct taaactacca agtgaagatt taatgcaaag tgtgttagac
    18481 aaagtcataa aaaatgaatt gactgttaaa aaaacagaaa aattgataca agatgtatta
    18541 gaagaaacta aagttcaaga agagcctgat aaaaaacaga atataaaggg tgctatgagt
    18601 ataagaattt acataaatac tataaagcag gcctttgatg ctatatcaaa tacaggaatt
    18661 gatgctaagt ataatgaaat agataaggga gattatatgg aagttgttgt aaaaatccct
    18721 aaaaaataga gattactgct taccagtaat ctttttttgt aatataagcc ttgtaatata
    18781 aactgattta tagacattat ttatacttaa aaaacaccta ttcaacattt aaaattaatt
    18841 taattcttaa aggggggaac atcatgggca aagttatagc tgtattcaat caaaagggtg
    18901 gagtcgggaa aactacaaca aatgtaaatt taagtgctag cttaggaact ttgggaaaga
    18961 aaatattagt attagattta gacccacaag gaaatacaac tagtggatat ggtattaata
    19021 aaaacgaagt agaaaataca atctatgaaa ttatgttaga tgggttacat attaaagaag
    19081 caataataag tactgaattt gagaatatag atgtagttcc atcagctact gaactatcag
    19141 gtgcagagat agagcttaca tctaaaacta atagagagta tattttaaaa aattccataa
    19201 aagcggtaat agatgaatat gattatatat ttttagattg tccaccctca ctggggatgc
    19261 ttacaataaa ttgtttaact gctgtagata gtgttttaat tccaatacaa tgtgaatatt
    19321 atgctttaga aggtgttagt caattaatgg agactataaa attagtaaaa tcaagattaa
    19381 atgctgatat agaaattcaa ggtgttgttt taagtatgtt tgatggaaga gctaatttat
    19441 caatacaagt agttgaagaa gttaagaaat actttaaagg tagtgtatat acaactttaa
    19501 ttccaagaaa tgtaagactt gcagaagctc caagtcatgg aaaacctgta atttattatg
    19561 ataaaagatg tagagggtct gtagcttatt tggagctagc agaagaattt atagatttag
    19621 aagaggagga atggtagtat ggagaataag tctaaaagaa gtaatagatt aggtagaggt
    19681 cttagtgcat tgataccaga aattaaagga gaaacttcag aaaaagaaat agtcaacata
    19741 gatatagaca aaatatatcc aaacgaagtt caacctagaa agcaatttga tgaagaaaag
    19801 ataaaagtat tgtctgactc tataaaaaat tatggtgttt tacagccaat agtggttaaa
    19861 atggatgaaa ataataaata tatgataata gctggagaaa gaagatttag agcatcaaaa
    19921 cttgcaaata aaaatcaaat acctgctata attaaagata tagatatgaa agatataatg
    19981 gagatagcct taattgaaaa cttacagagg gaagatttaa attcaataga agaggctcta
    20041 gcatataaga gtttaataga gcattacaat gtaactcagg aagaaatatc agaagctgtt
    20101 gggaagagta gacctcatat aactaataca ctaagacttt taaatcttgg acaagatgtt
    20161 atagaaatga tagacagtgg tagaattaca gcaggccatg gtaaggcttt attgagaatt
    20221 gcagataaag atttgcaact tcaaatagcg aaaaaaatag aagaagaaga actttctgta
    20281 agagaagtcg aaaatatagc taaaaaaata tctgaaaata aacaagaaga acctaaaaaa
    20341 tctaaaccta aggatgtatt tattttagat gttgaagata aacttagaaa tatatttgga
    20401 acaaaggtga atatttctaa aggaaagaaa aaaggtaaaa tagagataga atactataat
    20461 gatgatgatt taaatagtat tgtatcaatg cttcttgaat agaacaaaaa tataaaacag
    20521 agaggaagat tgttatgatt tatttagata atgctgcaac tacatatcca aaaccagaaa
    20581 gagtgtataa tgctgtttta gattgtatga aaaattattg tgcaaatcca ggtagagcag
    20641 gccataaact tgctatgaga gctgctaggg agatatatga tacaagagag aatatagcta
    20701 agttgttcaa tgtaagtaat cctatgaata tagtatttac atctaatgca acagattctt
    20761 taaatttagc gataaaaggt gtattacaag aaggagacca tgtaataaca acgtctatgg
    20821 agcataattc agtaataaga cctataaaag cattggaaaa aaggggtatt gaaaatactg
    20881 tagtaaaatg cgattatgag ggatttttag attatgaaga tttagaaaaa tcaataaaga
    20941 ataatactaa attgattgta acaacacatg cgtctaatgt atgtggaact ttaatagata
    21001 taaaaaaagt tggagaaata gctaaaaaac ataatatact atttttagta gatgcatcac
    21061 aaacagcagg tgtttatgat atagacgtaa atgaatgtaa tatagatatg ttggcaatgc
    21121 caggtcataa atgcttattt ggacctcaag gaacaggtat attatatgta agagaagggt
    21181 tgaacttaaa tatattaaaa gaaggtggaa caggtagtaa atcagaagaa atagttcaac
    21241 cagagttatt cccagataaa tatgaatcag gaactcataa tactccaggt atagcaggtc
    21301 ttaaccaagg tatattattt atatttgaaa gaggaatcaa caatataaga caacatgaag
    21361 aagaattatg tcaatatatg atagacaaat tagaggaagt accagatata aagatatatg
    21421 gaccaaagga cagtaaaaaa agagcatcag taatagctct aaatatagga gatatggatt
    21481 ctggagaggt tactttctta ttagatagtg attataatat agcgactaga tcaggaatac
    21541 attgttcacc actagcacat accactttag gtactttaaa acaaggtgca gtaaggttca
    21601 gtataggata tttcaacaca aaagatgaga tagataaagc agtggaagct ttgaaaaaaa
    21661 tttcaaaaaa taaatagcat gagatttaat aatatagaaa taaaaaaggg ggatatttca
    21721 aaataaaaat ttgaatcatc tccttttttg tacaaaaaag aaaaggggtc cctaagaacc
    21781 tctaatctat ttgataatag tttcaagaaa ttactttaag taatctcctt cttcaagaac
    21841 tatatgacca ctttcgtaat tagaaccatc aacagtaatt tttatttgtt caacattcat
    21901 attttttatg taagtacgag caactgaatc aagcattaaa gtttctgcat cactacctaa
    21961 gtttgacttt gtaaagtcag agctaacatc taatatacca gtttttacac catcaatgtc
    22021 ttttgtagta aatgaattta attttgcaga ttctggaaca actttaagtt tttgaagttc
    22081 ttcaaataaa gtgttctcat caattttatt taaatcaact ttattttcaa ttaaatctgt
    22141 cttatctaca tcaaaagagt aaatagtagc tgattgagtt ttagtttctt cttttggagt
    22201 aggtttagct ttgttattag gaacctcttt ttcagtattg ttttcttctt ttggttgctc
    22261 aacatttgta ttgctcttag tttctttttt agatgaatca cttccatttt gagcattagt
    22321 acaaccaact gcgaatatag atatagataa tatacttaac actaacatta ttttcttatt
    22381 tttcataaaa atttctcctc tcttaaaaac tattcttata tataattata gtcgttatga
    22441 gcaaatatat gaatacaaaa tagtaacaaa tttaaaaaat gatttattta aattaataaa
    22501 actcaaaata aactaaattt tagtcatttt tatgggaaat catttaatgt acagtgaaaa
    22561 tattgtaaaa taaaagtagt gtttttaaaa taaatataag ttataattat attataaaaa
    22621 aatgtcttca aaaggtggga atagtatgga aattttgagc ttaggagaaa agattaaaaa
    22681 attaagaaaa gaaaaaaaca tgacattaaa agaattagct ggagatagga taacagcagc
    22741 acaaataagc catatagaga gggataaatc tcatactagt tatgaactgt tggaatatct
    22801 aggcgataag ctagatgtaa gtgtagaata cttattagag accaaggaaa tgcaatcaaa
    22861 aaagataact gataatttaa tactacagag cgaaatttat ataaaagaaa atgaattaaa
    22921 caaggctgag gaccaaataa aagaagtttt agatatatgt aagagatacg atttaatgga
    22981 gaactatggc agatgtaatt ttttaatggc taatattaat ttgaaaaaat ctaattataa
    23041 tgatgctata gaaaactttg aaaaggcatt gtacttcttt ataaaaaata gtgatagtga
    23101 aaatatactt aagtgttatt taaacatagg gaaaatatat atgcaggaag acttttataa
    23161 aggtgctata agccacttta attttgcaga ggaaattttg tcagagaata aatttgagga
    23221 tgttaacata cacaaggaac tatatagtaa gatggcatat tgttacataa aactagataa
    23281 tccaaatatg tcattgaagt acatagaaaa gataaatgaa atagataata gaaatgatag
    23341 aaaagaagat gtcgatattt tggtgttaaa agcaaataat atgcttgaga ttggaaaata
    23401 tgaagaatca agagattact ttaaaaaagc actaaaaata cttgagagtg aggataataa
    23461 aactggatta gcaaatgtgt atttaacaat ttgtgatgtt tacagaaaaa taggtgaaac
    23521 agatagagta cttgaatatt cacaaaaggt atatgacata aagaaaaatg atgaagatga
    23581 atacatgatg tcaggattat ttaagataat agaagcatat atagataaag aagattatga
    23641 tttagctaga aaatattgta agatagcctt agcttcatca ataaaaaata aaaataaatt
    23701 caatgaatat aaagcattga gattctatgc taatatgtat aaaaatcaga atgaagtaac
    23761 tcttgcaatt gaatatctga ttaaatgcat aaaaatagta tcagatttag gcaacaatag
    23821 aatattggcc aatttatata tagacttagg tcaattatat tcaaatatat caaaagaaaa
    23881 agagttagaa tattatcaaa aaggtgtatt tatgtacaaa aatttagaaa taatgtaggg
    23941 taaataaacc tataagggta tagaaacaat ctataacaag caagataata tctatgataa
    24001 aatataaatt gtatgaatat ttttaaggag atgaatcaga caatgagtat aaaaatagat
    24061 gcgagagggc ttgcttgtcc taaaccagtt ataaatacaa aaagagaatt ggataaccta
    24121 gaagaaggtg ttgtagttac aatagttgat aatgaaacag ctaaggagaa tatattaaaa
    24181 cttgctaaat ctttaagttg tgaagcaaat attgttgacg aaaaagagaa ttttatatct
    24241 atagaaatta gaaaaggtaa aaatatacta gataaacaaa atgttgtaga taataaagaa
    24301 agtgaattag acaatacatg tatatttata tcttcagata agatgggact tggaaatgac
    24361 gaattaggaa aagtgttaat aaaaggattt atatatacat taacagaatc taaaccttat
    24421 cctaagtatg tacttttagt taatggagga gtaaaattaa gtgctgaaaa tgaagaaacc
    24481 atagaaaatc taaaaatact tgagaatatg ggtgtagaag ttttatcttg tggtacttgt
    24541 ttagattatt ataatttaaa agacaaatta caagtaggta cagttacaaa tatgtatact
    24601 atagttgaga cattaaagaa tgcatcaaat accatttcta tataatgtat gtatgtttaa
    24661 taatataaaa ctaacaataa agattaactc aaatagatag tcagaaagga ttaagtaatg
    24721 aatcaaatgt atatagtgtc atttaattcg acacatcatg cgataagatc agaaaagtta
    24781 tttggtgaaa atagtctaaa agtaatggct cttccaacac caagagaaat aacagcaagt
    24841 tgtggaatat caattaaatt ttcttttgaa gatatggaaa agataaaaac tattttggtt
    24901 gaaaataatg tagatataaa aggaatttat tgtataagta aattagaaaa tggttcaaaa
    24961 gaagtagaga agttagatta ggaggctaat tatttatgcc aatggattta aaaataggtg
    25021 atattgttga attaaaaaag caacatgcat gtgggtgtaa agagtttgaa atcataagaa
    25081 cgggaatgga tataaagata aaatgtacta aatgttcaag gctaataatg cttgatagag
    25141 agaccttaga aaagagagta aaaaagctaa taaaaaagtg ataaaaattt aacacttaaa
    25201 aaatgtcaaa taccgtatat taatatccaa aattttggta taataagaat tgaagaaaac
    25261 attttcatgg gggtgcttta attatggcag ttaaatatgc aaaaagaatg caaggattac
    25321 aaggatcaga aatacgtgag cttttaaaac ttactcaaca accacaaata atatcttttg
    25381 ctggtggaat gccggctcca gagttatttc cagttgagga aatgaaaaaa gtatcagttg
    25441 cagtgcttga agaaaatgga agatcagcta tgcaatatac tacaacagaa ggatatgaac
    25501 cattaagaga aaaaatagca gctagaatga atgataaaaa caaaactaat gtaaacaaag
    25561 atgatatatt agttacaagt ggttctcaac aaggtctaga ttttgcagga aaagtattca
    25621 ttgatgaggg tgatgtaatt ttatgtgaaa gtccatctta cataggagct ataaacgcat
    25681 ttaaatctta tcaacctaaa ttcatagatg ttccaacaga ttctgatgga atgataatgg
    25741 aagaattaga aaagatacta gaaacaactg atagaataaa aatgatttac gtaatacctg
    25801 acttccaaaa tccaacagga agaacttggc cacttgaaag acgtaaaaaa ttcatggaaa
    25861 tagtaaataa atttgaaata ccagtaatag aagataatcc atatggagat ttaagatttg
    25921 aaggagagac tttaccatct ttaaaatcaa tggatacaaa aggattagta atattcttag
    25981 gaactttctc taaaatattc tgcccaggat acagattagg atggacttgt gcttctccag
    26041 aaattttatc taagtttaac tttgcaaaac aaggtgcaga cttacaagca tcaactatat
    26101 ctcaaatgga agtaagcaaa tttatggata tgtatgacct agatgcacat gttgacaaaa
    26161 taaaagcagt ttatgttaaa cgtagagatg taatgcttaa gactatggaa gaagagttcc
    26221 cagaaggatt agtatttact catccagaag gtggattatt tacatgggta gagcttccaa
    26281 gtaatttaaa tgctaaagaa ttaatgccaa aatgtttaga taagaatgtt gcttatgttg
    26341 ctggaggagg attcttccct aatggtggta gagaaaatac tttcagactt aactattcaa
    26401 atatgccaga agaaaaaata atagaaggta taaaaaatat agcagctgta ttaaaagaag
    26461 ctatgggtgt agaagcttaa ttaaaaataa taacttatat ataatacaag ggatggttgt
    26521 tgccatccct tgtttgtttg aaaagtatat aattcacctt atactaaact ttgtccaaaa
    26581 tttaaattga cagaaataag aataaatgat aaaatgtgta ggtatgagat tttagtctca
    26641 tttctctgct ctaagagtgt agagccgtta agtccaaagg gaggtgaatt atagtgagaa
    26701 attatgaatt agtttatgtg gtaaagccaa actctgatga agaagtaaga gaggctatac
    26761 taaacaaagt taaggaagtt gttgcaactg acggagaaat agttaaagtt gatacttggg
    26821 gaactaaaaa attagcttat ccaatagcta agtttacaga aggtttctat gtattagtta
    26881 acttcaaatc agcagtagac gttcctaaag agatagacag aaacttaaag ataaatgaaa
    26941 acgtaataag acacatgata gttgttgctt aataactggt tttattataa aaaataggtg
    27001 gtgtttttat gaatcaagtt gtattagtgg gaaggttgac tagagaccct gaattaagat
    27061 acatcccagg tacaggaaca ccagtggcat cctttacaat agccattgat agagactatg
    27121 taaaaaaaga tggatctaaa gaaactgatt ttatacctgt tgaagtaatg ggtaaatctg
    27181 ctgaattttg tgctaactac ataactaaag gtagattagt agcacttcaa ggatctataa
    27241 gagtagacaa ttatcagaca caatcaggtg aaaagagaac ttttacaaaa gtaagtacta
    27301 gatcagtaca agctttggat agtaagaata aatcggaaaa ttcatataga gagagtgctc
    27361 cagcttttga gccaagcttt gaacctcaag gtttagaccc acaaggtttc caagctatag
    27421 atgatgacga tataccattt taaaaaggag ggaaaaaagt catgatgaat aagaaaagac
    27481 gtaagaaaaa aagagtttgt caattttgtg ctgacaaaaa cgctaaaata gattacaaaa
    27541 gtactcaaag attacaaaag tatataactg aaagaggaaa gatattacca agaagaatat
    27601 ctggaacttg tgctaagcat caaagagaat taacagttgc tataaaaaga gcgagaaata
    27661 tagcactttt accatataca ttagactaaa aaaatgagcc ttgtgctcat tttttattat
    27721 atgtatttat atatctaaat gaggcgagct ataaagctcg tttttttgtt tatatactta
    27781 aatatattat tatttatgat ataatattat atattaagga gggttgtaaa cgtgaaaata
    27841 gataaaggtt ctgagataac gagaaacata aagataatag aatggatgaa aacagaaata
    27901 ttaatgagtg taagtgattt gtttaattta ttgtttaaag gggtaaaacc attagatgaa
    27961 gcaattcaag atactttagc aaatataata atgataacat atctcttagc aaagagatta
    28021 ggaattagtt ttagagatgt ggattataaa gtcaaggaaa aaataaaaat tggtatagat
    28081 gaggaccata gcgttgaaag atggtatggg gatttatcta atttaaaaaa acatatggat
    28141 aatagggagt gatattaaat tgaataataa aattagacta tctaaggcaa tgtctataat
    28201 agttttagct ataataatcg ctttagtaat agcctatatt ccaatgctag gaatatttag
    28261 tatgttagca gctgttccat atgtaattat aggtgctatt actgatagaa agtattctct
    28321 tatatctatt ttggttacat tttgtgtact gattctattt gcagacattt cttatgcatt
    28381 aaatatatgt ataatgtatt ctttaccagg tatagtaata ggaaagatgc taaagagaag
    28441 ctttgaacaa gaggacagta ataaattcga accgatatat ggtggaacca taatatttgt
    28501 actttcaatg cttgtatatt tttttgtatt aaagacattt ttaaatgtga atctattaga
    28561 tgaagtttct aagatgatat cagaaattgt aaatattcaa aaaaatagtt tttctgcaac
    28621 tgaacttaaa gtgtttgata aaatgaagcc agatgagatt gtaagttatt ttacaaatat
    28681 gctaccaatg atgttatttt tacaagggtt gttatcagcg tttgtcacat attatttgag
    28741 tgtatttttt ataaaaagga ttacaaagat gaatataaga tttcctaagt ttgctgattt
    28801 ttatttaccg ggaaatgcta tactcacaac tttcttactt tatttattag tcttatttat
    28861 tcaaattatt ggttcaaaat tatatacaga attaataatg actaatttac agctagtttt
    28921 taatttaatg tttgtaattc aaggtatagc agtttgcatt tatttcttaa agaaatggat
    28981 aagacaggga ccaaataaaa ttatgttttt ttcagggata attttatgtt tatttggatt
    29041 tatgggaata tcttttgttg gtatggtaga tagtataatt gactttagaa aggtaagaag
    29101 ctataaatcc acttaggagg atagaaatga gtaataaaca aacctttaaa ttaaatatgc
    29161 cagaaataaa tttatatata attgttattg gtatttctag tataattttg ctttattaca
    29221 acttgtatgt agggtgttta tttttctgta tttttgtata tatggttttt cacaattgga
    29281 gaactactaa tattagacgt catgaatgga cagagtatat tcaaaattta tctttagata
    29341 tagatgaaac aactaaaaaa gccataataa atcttccaat accattatgt attttagaat
    29401 ttgatggaaa tatatcctgg tataatggaa agttttatga tatgattggt caaaaagatt
    29461 tacttgataa aaatatagag gatatagtta aaaatcttaa tttaagaaaa gtattaaatg
    29521 aaaataaaga gatgtacaca gaaataaact ataaagaaaa agaatataca ataatttata
    29581 atgttataaa aaatgatcaa gaaaaaaatc ctaaatattt gatgatatta tactggatag
    29641 acaagactga atatttaaaa gtaaaacaaa attatgatga tgaaaaaaat gcaatgatgc
    29701 taatacaagt agatggatat gatgaagtat taaaaagtgc agcagaagat aagagagctt
    29761 taattaatgt tgaagtagag aaaatacttt ctgctttaga attaaattca aatggtgcat
    29821 tgagaagaac ttcaaaagac aaattcttct tggtaatgca taaaaaagag ctaaaaaaat
    29881 tggaagctga gaagttttct attttagata caataagaca tattgattat ggaaataatc
    29941 ttcctgttac tataagtata ggaattggta tagatggaga tacattaaat gagaacctaa
    30001 aacttgctac gggtgctctc gatttagcac ttggtagagg tggtgaccag gctgttgtaa
    30061 aaacaaaaga taaatttgtg ttttatggag gaaaatctaa agctgtagaa aagaaaacca
    30121 aagtaaaatc cagattaata ggtcatgctt taagagaggt tatacaacaa agtgaccaag
    30181 tttatataat gggacataaa tatcctgata tggatgctat gggagcagca gttggtgttt
    30241 atgatatatg taaatcctgt aacaaaactg caaacatagt tttacaatct gtaaatgaat
    30301 ctatagaaat atttataaat aaaataaatg aaaataatta ctataaaaag ttgtttattg
    30361 gaaaagagga agctattgac aactgcacta aaaatacatt agttgtagta gttgatactc
    30421 atagaccaaa ttatactgaa tgtgaagaac tgttaaagct atcagagaag gtagtagtta
    30481 tagaccatca tagaagaggt gtagagttta taaatgatgc agtgctttta ttccatgaga
    30541 tatatgtatc ctcaacgtgt gaaatggtta cagagttagt tcagtatatg gatgaagatg
    30601 taacaataaa taaacttaca gctgaagggc ttttagcagg tataagtcta gatactaaaa
    30661 actttgcatt caaaacaggt gttagaactt ttgaagctgc ttcttacctt agaaaagttg
    30721 gagcagatac tatagaggta aagaaattct tcaattctga tgtaaaagat ttcataataa
    30781 aagctgaaat aattcaaagt actaaaataa ttaataatag aatatgttta gcatattcaa
    30841 gtactgagat agatagtata aatgttataa tagctcaaac tgctgatgag ttattaaata
    30901 tcaaagaagt agaagcttcc tttgtattag gtgaaaaaga tgatactata tttataagtg
    30961 cgaggtcttt aggacaaata aatgtacatg tacttatgga aaaattagga ggcgggggac
    31021 acatagatat agctggagca cagttaaaaa atgtttctct aaaagaagct tataagatgg
    31081 taaataaaat aatagaggag tatttggagg aggaagaata gatgaaagtt atattattaa
    31141 aagacgtaaa aggaactggt aaaaagggag aaatgaaaga agtaagtgat ggatatgcaa
    31201 gaaatttctt atttcctaaa aagatggctg tgcaagcaga tagtgtagca ataaaagaat
    31261 taaatgaaaa gaataaatct aaagaaataa aagcacaaaa agagtatgaa gaagcagttt
    31321 tattaggtaa gcaaatggaa gaaattaata tagaaatata ttcaaaagca ggagaaggtg
    31381 gaagattatt tggttcaata acttctaaag aaattgctga gcaattaaag aaacaaaaag
    31441 acatagatgt agataaaaga aaaatattat tagatgaacc aataagatct ttaggttcaa
    31501 cttttgtaga aataaaaata catcaaaaag taactactaa aataagagtg gatgtaaaag
    31561 aaaaacagta ataactagag gtgattaaga tggaagatat gacgagaatt cctcctcata
    31621 gtgtagaatc agaacaatca atattgggtt ctatacttct agataaagat gctataataa
    31681 cggttacaga aactataaag cctgatgatt tctataaaga ggctcacaaa ataatatatg
    31741 aatgtatgat aactttaagt aataaaggtg agcctataga cttgataaca ttaacagaag
    31801 aactaagaaa acaagggcat cttaatgata ttggaggaat cagttatatt acaagtcttt
    31861 ctactatagt accgacgact tcaaatgtaa aatactatgc tgatatagta aaagaaaagt
    31921 ctgtacttag aaagcttata aaagcttcta atgaaataat aaatctagga tatagtggag
    31981 caactaagat tgaagatgtt ttagaacaag ctgaaaagag tatatttgat atatcacagg
    32041 agaaaactag tgatgatttt aaatctataa acttagtttt gatggatgcc tatgatatga
    32101 tagaaaaatt atacactaac aagagtgatg tcactggtat aactactggt tttaaagatt
    32161 taaacaaaaa aataaatggt cttcaaagga ctgatttaat attaattgca gctagaccag
    32221 caatgggtaa aactgccttt tctttaaact tggttcaaaa tgcagcctta aaaggtgatg
    32281 cttctgtagc tgtatttagt ttagagatgt ctaaggaaca attagtacaa cgtatgttat
    32341 cttcacaatc tagtgtagaa ttaaaaaaaa taaagactgg aacacttaat gataatgatt
    32401 ggcctagaat tatagatgct atggcagtat tatcagatgc caaaatacac atagatgata
    32461 ctcctggtat aaaaatatca gaactaaggt caaagtgtag aaagttaaaa atagaaaaag
    32521 gactagattt agtgcttatt gattatcttc aacttatgga aggtgaaggt aataatgaaa
    32581 gcagacaaca agagatatct aagatatcaa ggtcactaaa aatattagca aaagaattaa
    32641 actgtcctgt agtagcacta tctcagttat cacgtgcacc agaacaaaga gcagaccata
    32701 gacctatgtt atctgattta agagagtctg gagcaataga acaggatgca gatatagtta
    32761 tgttcttata cagggacgaa tattatcatg ctgattctga gagtaaaaat ataggagaag
    32821 taataatagc aaaaaataga cacggtgaaa ctggttctgt ggaacttgtt tggcttggtg
    32881 aagttcaaag atttggggat aagttgagag acctataatc taacgaatat ccacagaacg
    32941 tcctttcttt ctacaggaaa aatgtggata catgtcacta aatatgtcca aacattgaaa
    33001 tttagctaaa aaactatcca cactatccac agatggcctg ttgataagtg tgaataagta
    33061 gataaagcaa gcttttggct tgctttatct gtgtataaat attaattatt aggaggaata
    33121 atatggaaaa aataagctta aaatatatat atcccaatat aattaaagta cttgatgaaa
    33181 taaacttatt tagagtaatt gacaataatt taagagaatc tattgttgtt tatgctaata
    33241 atgtggataa tcagtatcat ataaacatga ctaatacaaa ctttggaaac ataattaaca
    33301 tatgtaaatt agaaaaactt ttagatgtgg ataaatttat ggaaaaagtt ataaaatatg
    33361 aaaaagaaat aatagagaaa gaagagtttt caaaaataga agaatatatg ttaaatattg
    33421 gagaatacta aaaaagttga aaaaaatacg aatgtttgat agaataagta agtaaagtgt
    33481 tcgagagtat ataaaatata gaggtgaatt acatgaaaac agttgcaatt gttggttctc
    33541 aatggggaga tgaaggtaaa ggtaaagtta tagattatct tgctactcaa gcagatgtag
    33601 tagtaagagg acaaggtgga aataatgcag gacatacatt agttgtagaa ggtaaaaaat
    33661 acgcattaca tctgattcca tctggagttt taaatccaaa tacagtgaat ataatcggaa
    33721 atggaatagt gtttgaccct aaaggatttt tagaagaatt ggaaatgttt aaaacggata
    33781 atataagtac agaaaacata aaaataagtg atagagcaca tgtcatattc ccatatcata
    33841 aagaactaga tgcattgtca gaagaagcca gaggcgattt aaagataggt acaacaaaaa
    33901 aaggtattgg accttgttat atggataaaa cagagagatc tggtattaga atatgtgatt
    33961 taatggataa agataaattt gctataaaat taaaagctca aatagatgct aaaaatgaaa
    34021 tagttaaaaa tatatatgga aaagaagaat tatttgattt tgaaactata tataacgaat
    34081 atctaggata tgcagaacaa ataagaaaat atgtggcaga tacgtctgtt attgtatatg
    34141 atgcagtaag agcaggtaaa aaagtattgt ttgaaggagc acaagggact ttattagatt
    34201 tagatttagg aacttatcca tttgttacat cttctcatcc aacatctggt ggatttgcaa
    34261 ttggtgcagg tataggacca aatatgataa aagacgttgt tggtatagta aaagcatata
    34321 ctacaagagt tggagaagga ccgtttgtta cagagcaaat aaatgaaact ggagataaga
    34381 taagagaaca aggacatgaa tttggggtaa ctactggtag accaagaaga tgtgggtggt
    34441 ttgatgcagt tatagttaag tatgcagcta gagttaatgg cttgactagt atttcattta
    34501 tgttacttga tgtattaact ggatttgata agataaaagt ttgtacgtct tataaaatgg
    34561 gcgataaaat aataactgat ttcccagctt cattagatga tttagcaaaa tgtgaacctg
    34621 tatatgaaga attagatgga tggaatgaag atataactca aatagataac tttgatgacc
    34681 ttccagaaaa tgcaaagaaa tatatagcaa aaatagaaga actagttgga gtaagtgtag
    34741 atatggtttc tgttggtcca aatagagctc aaactataat aagaagaaat atatttgctt
    34801 aatattttat caattaaata atttaaatag taaatagttg aagaagagta tctcaatata
    34861 atgtttatag ttattagaga tgctctttta tgtttgactt acattgattt tttattaagt
    34921 taaaactact gatacacatt ctaactgaaa ttaaagttga atttgtgtag atacatatag
    34981 gttttttaaa aagaattttg ttgaaaataa atagctaaag atgattttaa ttatatgcta
    35041 aaaaaagtaa aaaaaactat tgactaaaaa ttgtatagac gctataatat aacttgtgag
    35101 tgaaagaaac gacgaaaaaa gtcgaaaaaa tgtggtctat tagctcagtc ggtagagcac
    35161 ctgactttta atcagggtgt cccgcgttcg agtcgcggat agatcaccaa aatatggctc
    35221 gttggtcaag a
//
LOCUS       DAEORZ010000029        34807 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEORZ010000029 DAEORZ010000000
VERSION     DAEORZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.029"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            39..776
                     /gene="cas6"
                     /locus_tag="KRH39_001273"
     CDS             39..776
                     /gene="cas6"
                     /locus_tag="KRH39_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG8511191.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRNPLEFIPLNFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            781..2256
                     /locus_tag="KRH39_001274"
     CDS             781..2256
                     /locus_tag="KRH39_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511192.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIVKNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     VNTKNLGKKMIDNWNLSNLKELLLDNISVLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGEEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2276..3289
                     /locus_tag="KRH39_001275"
     CDS             2276..3289
                     /locus_tag="KRH39_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HBG8511193.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIIFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYIGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            3305..4102
                     /gene="cas5"
                     /locus_tag="KRH39_001276"
     CDS             3305..4102
                     /gene="cas5"
                     /locus_tag="KRH39_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG8511194.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLKSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVMPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4129..6537
                     /gene="cas3"
                     /locus_tag="KRH39_001277"
     CDS             4129..6537
                     /gene="cas3"
                     /locus_tag="KRH39_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HBG8511195.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQKEIYDYIE
                     KGILEKEDFNLEDIEDYIKEILDVDFKFNEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDDMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKDAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            6978..7925
                     /gene="prmA"
                     /locus_tag="KRH39_001278"
     CDS             6978..7925
                     /gene="prmA"
                     /locus_tag="KRH39_001278"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBG8511196.1"
                     /translation="MNNWIEITIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            7944..8702
                     /locus_tag="KRH39_001279"
     CDS             7944..8702
                     /locus_tag="KRH39_001279"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBG8511197.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8704..10002
                     /gene="mtaB"
                     /locus_tag="KRH39_001280"
     CDS             8704..10002
                     /gene="mtaB"
                     /locus_tag="KRH39_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBG8511198.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKNINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10045..10395
                     /locus_tag="KRH39_001281"
     CDS             10045..10395
                     /locus_tag="KRH39_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBG8511199.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10558..10737
                     /locus_tag="KRH39_001282"
     CDS             10558..10737
                     /locus_tag="KRH39_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBG8511200.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10767..11210
                     /locus_tag="KRH39_001283"
     CDS             10767..11210
                     /locus_tag="KRH39_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBG8511201.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11321..11818
                     /locus_tag="KRH39_001284"
     CDS             11321..11818
                     /locus_tag="KRH39_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBG8511202.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12011..12574
                     /gene="raiA"
                     /locus_tag="KRH39_001285"
     CDS             12011..12574
                     /gene="raiA"
                     /locus_tag="KRH39_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG8511203.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            12787..13023
                     /locus_tag="KRH39_001286"
     CDS             12787..13023
                     /locus_tag="KRH39_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBG8511204.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13036..14379
                     /gene="yqfD"
                     /locus_tag="KRH39_001287"
     CDS             13036..14379
                     /gene="yqfD"
                     /locus_tag="KRH39_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBG8511205.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14318..15433
                     /locus_tag="KRH39_001288"
     CDS             14318..15433
                     /locus_tag="KRH39_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBG8511206.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            15490..15951
                     /gene="ybeY"
                     /locus_tag="KRH39_001289"
     CDS             15490..15951
                     /gene="ybeY"
                     /locus_tag="KRH39_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBG8511207.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            15957..16676
                     /locus_tag="KRH39_001290"
     CDS             15957..16676
                     /locus_tag="KRH39_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBG8511208.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            16795..17196
                     /locus_tag="KRH39_001291"
     CDS             16795..17196
                     /locus_tag="KRH39_001291"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG8511209.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17260..18153
                     /gene="era"
                     /locus_tag="KRH39_001292"
     CDS             17260..18153
                     /gene="era"
                     /locus_tag="KRH39_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBG8511210.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18165..19544
                     /gene="mgtE"
                     /locus_tag="KRH39_001293"
     CDS             18165..19544
                     /gene="mgtE"
                     /locus_tag="KRH39_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBG8511211.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19617..19772
                     /locus_tag="KRH39_001294"
     CDS             19617..19772
                     /locus_tag="KRH39_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBG8511212.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            19738..20511
                     /gene="recO"
                     /locus_tag="KRH39_001295"
     CDS             19738..20511
                     /gene="recO"
                     /locus_tag="KRH39_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBG8511213.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20504..21103
                     /locus_tag="KRH39_001296"
     CDS             20504..21103
                     /locus_tag="KRH39_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBG8511214.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKVKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21263..22141
                     /gene="glyQ"
                     /locus_tag="KRH39_001297"
     CDS             21263..22141
                     /gene="glyQ"
                     /locus_tag="KRH39_001297"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBG8511215.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22141..24207
                     /locus_tag="KRH39_001298"
     CDS             22141..24207
                     /locus_tag="KRH39_001298"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBG8511216.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKTMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFDKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKIKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(24373..25920)
                     /locus_tag="KRH39_001299"
     CDS             complement(24373..25920)
                     /locus_tag="KRH39_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBG8511217.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DTFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            26200..26967
                     /locus_tag="KRH39_001300"
     CDS             26200..26967
                     /locus_tag="KRH39_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG8511218.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            27250..27447
                     /locus_tag="KRH39_001301"
     CDS             27250..27447
                     /locus_tag="KRH39_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8511219.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            27476..28552
                     /gene="vorB"
                     /locus_tag="KRH39_001302"
     CDS             27476..28552
                     /gene="vorB"
                     /locus_tag="KRH39_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG8511220.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            28555..29274
                     /locus_tag="KRH39_001303"
     CDS             28555..29274
                     /locus_tag="KRH39_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG8511221.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            29287..29826
                     /locus_tag="KRH39_001304"
     CDS             29287..29826
                     /locus_tag="KRH39_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG8511222.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            29983..31041
                     /gene="buk"
                     /locus_tag="KRH39_001305"
     CDS             29983..31041
                     /gene="buk"
                     /locus_tag="KRH39_001305"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG8511223.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            31059..31952
                     /locus_tag="KRH39_001306"
     CDS             31059..31952
                     /locus_tag="KRH39_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG8511224.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANETVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            31982..33190
                     /locus_tag="KRH39_001307"
     CDS             31982..33190
                     /locus_tag="KRH39_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG8511225.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            33214..34473
                     /locus_tag="KRH39_001308"
     CDS             33214..34473
                     /locus_tag="KRH39_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8511226.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT        14061 a         3536 c         6428 g        10782 t
ORIGIN      
        1 agaataatga attatactag aaagtaggag gtgaaaatat gagcagaatt tcaattttta
       61 tggaatcatg taatggcaaa tttccaaatg aaaattcaat gttgtcagtt tcctttataa
      121 aaaatatttt aaatgcagaa aacaaagatt ttgctaaaaa catatttaac tatggggaag
      181 aaagaaaaaa caataagcaa ataaaggata taaatacagc catttacata ccaaatctta
      241 taagaagcaa aaatgagtta cttgttgatg gagatataat attcaatata agtttttata
      301 attattctat gttcagtaaa ttatacaatg gaattttaaa agtaaaggag ctagaatata
      361 aaggttatag atttaaaata aaaaatataa aaatacataa agaacacgag ataaaagaaa
      421 atggtgttat atttaaaact atgtctccta taatagtaaa gaataaagaa ggtaaatatt
      481 tagatgtaga agattcaaat tatatagaat gtttaaacta tatagctaat ctaactctgg
      541 agagtataag aggtagtggg cttagaaatc cattggaatt tatacctctt aacttcaaaa
      601 aaagagtgtt aaaagaaaag attcgtggat ttaaagtgag agaatattac tatataaatg
      661 cttatgcagg aacatttttt ttaaaaggag atatggaaga tttaaatgca ctgtataaaa
      721 tgggaattgg atatagaagg acggaaaatg caggaatggt tgatatatta aagtagcgag
      781 gtgagagaaa taaaagctaa attttataga tcaggtgata ttgtaagaga tattggcatt
      841 gtttactttt atgaattact aatggattta aaaaacgaac tagaaaaaaa tgattataag
      901 ctaaaattta cttgtgagtt aaatagaaat tatttatctt tagaatctgc agaatatata
      961 gaccctaagt atatatctga ttatatatta gaaaatcaac tttttaaggt atttaaaaat
     1021 gggaaaaagg aaaccttaga aaaaatattt agtaatatag ataatttaac atatgtaaat
     1081 tttattgaag aattagataa atcttctgca actgagaaac aaaaggaagg gattaaaaaa
     1141 gattttaaaa acagaagatt tccatatgtt agaaatagtg gtaagtatgg attaaacacg
     1201 agtttagaaa atatggaaac aaatgtaaaa agaatagtag atactgtaat taaatcaaat
     1261 atagagatag atgaaataga taaacttgat ttgacacaat atgaagaaaa agatagtgta
     1321 tgtaatgttt gcaatataaa taaaacaaca aaactagata ttgatttaag agaacgaaag
     1381 gattctaagt ataacttttt atttagaggt gcagaaaaat caggttttaa aagaagtgga
     1441 caagttgaaa gtaatatttg ttttgagtgt gaatttttca acttaatgtg tcttttatat
     1501 ataaatttaa aaagaccaat ggtttttgca tatacgaatg atttaagaga attagcattt
     1561 ttaaatcata agataatgct taaaagaaaa atgtattcag ataaatcatt ttataaaaaa
     1621 cttttgcatg aaaaaataag ttcaattaga ctatatagat ttgatattgt taagaataaa
     1681 ggtataattc taaagtttga ctctattata gagtataaag aattattaaa aaaaattgaa
     1741 cttatagata ttatagataa ttacaatttc tcaagggagc cagtaaacac taaaaattta
     1801 ggtaaaaaaa tgattgataa ttggaattta tcaaatttaa aagaattatt acttgataat
     1861 ataagtgttt taaagcaatc aactggaact tctcgagaaa tggattttgt atctagctca
     1921 tataatatcg gtttatatat aaaattgtgt tggattgtag atggagagga ggagtataaa
     1981 atgaaaaact atatggaaag taatttaata tattcaagag taggtaaaga cttatttaat
     2041 aaaatgacag atgaatccag aagaaaaaat ttttctatga aagtgattca actacttaag
     2101 tctaatgata gaactcagct atttcaaact ataatgcatg tgttagtttc taatggaatc
     2161 ttaataggag aagggtttgt agaaggtcta atgcagagtt ctgaattaga attaaatacg
     2221 aatgtaggtc tatttattca agaaatcatg aaataaaaaa gggggaaaat ttaaaatgaa
     2281 tgaaaaaaga gtaaaaggag taacattaac tatactgaca gaaagcccag ttccactttc
     2341 atatgaccaa gggtatggaa attatacacc aataaaaaaa gaacaatata gagaaaaaat
     2401 acatgcaaaa actagtatag ctacaataac atatgactta agaagaatgc tacatcaaga
     2461 atatggttgg aatttaagta atattatttt tgggaaaaaa ggaaatatat atccatctat
     2521 taagaaaaat gtaggatgtg tagatgaaaa tggattagag actgatgtat ttggttattt
     2581 gataccactt aaagataaag gttctataag taaagcttct ccacttagaa taataccttt
     2641 taggtcatta aatccatata taggttcaac acagctaata acaaatagag gttttttaag
     2701 tagcgaattt ggtagaaaat attatgatga gaaggaagaa aatgaagtac ctagagatga
     2761 aaactttcca acaacacaag cattagcaat agaagagact ttatcagatt actacgttta
     2821 tacaataaca ttggagcttg atagaatagg agtagtagaa gtagaagatg ggaagttact
     2881 tttaccagaa gaaagaaaat ttatgagtaa ggaattaaga gaaaaggcag taaaagatat
     2941 attagatgca attactgtgt ttacaagaaa cataaaacat tcatcagtac tattaaaacc
     3001 acttgcagta atgggtggag cttttgataa agttgtaccg tttttttggg atgatgtaga
     3061 ttataatgct gatagtggtg agataaattt agagggagtt atagaaacta tagaaagtta
     3121 tagccttaaa gaaagtaata caatacttgc tatcaatgac agattaaaaa tttctaataa
     3181 aaaggaatta aataagttta atttaagtaa ataccctgta aaagagatta aaaatctagc
     3241 agatagattg gaaattggag aagataatat gtggtattta aaagagtagg aggttttttg
     3301 aattatgaaa gtattacaat gtaaacttaa gcaaccaact gctcactata gagacccaaa
     3361 agtatttcaa aatgaatata taagtacttt aaatttacca tctaagacta caataatggg
     3421 gatgataacg tatttatgtg atagaagatt aaaatctgat atagatatag gaataattgg
     3481 aacacatcat catagagaac tagaattttc aagaggagag aacattgatt tttggaatga
     3541 atatacaaat atgagaaaag gcaaagataa agaaaagttt ttgcttcaag gaaactacta
     3601 tgattactat aaagaacata aagctcaaaa tagcatatta aactatgaag ttcttaaaga
     3661 agtagaactg actatattta tttcttgcaa ggatgatgaa gaacttgaat ttattaaaaa
     3721 aaagcttgaa agtccatgta aatatgcaaa tttaggaaga aaagaagact ttgtaatacc
     3781 aagtgaaaaa gggtgttttg tgaaagaagt tgagttaaaa gaggttatgc ctatgaatac
     3841 tcgtgatgct ataaaagaaa atataaaatt aaagaataca tatgtaagag ttgacttaag
     3901 agataaagag aatgtcgaga ctataataaa tcaaggtgta ttgattgcat tacctcataa
     3961 atataaagat ttagaagcaa atagagatga tagagtttat gagttttgtc attatatata
     4021 tgttgataat gatggtatat atcctaaaaa tattaaggta aatgtatgta ttgatacaaa
     4081 agaagttttt acatggcttt aaattatttt gaaagatagg tgtttatcat gaaaaatatc
     4141 atgcaaaaag aaatattagc taagtcatac aatttaagaa ctaatcaaca agaacaaaca
     4201 ttagttgaac atattaagga tttatttgaa gtattagaat ctatattaga actaaatcta
     4261 tattcagata aagatgtaga gatactaaaa atatgttgtg ctttgcacga tttaggaaaa
     4321 attaattcaa ttatgcaaca aaaaatggag attaataata aaatatcgta tagttgctca
     4381 gaagaagaga ggaaaaagtt agagtctgac aaaaaatctt taaaaaagat agcaagacat
     4441 aatatatttt ctggagcatt tttaaaagat atattagaaa aaatgaactt gtcagaagaa
     4501 gataagttat atatatataa aagtataatg ttacatcatg gaaactatga agattatatg
     4561 aggttaagta ttagtaaagt tcaaaaagaa atttatgatt atattgaaaa aggtatttta
     4621 gaaaaagaag attttaattt ggaagatata gaagattata taaaggagat tttagatgta
     4681 gattttaaat ttaatgaaga tgtattggat tatgattaca tagataaatt aagtgaaagt
     4741 atgtttatag acagtgatta taacaatggt caaatagatg attcagtttt aaattataga
     4801 aagtttaagt atattttata taaagggatg ctgaacttaa tagaccatag tgcatcaagt
     4861 agacagaaag gtataaaatt ttataacgat tttactgatg aagaaattga tgatatgatt
     4921 ttaaatgaga tatataagag tcagggtaat ttagaaaaaa ataatataga gttcaatact
     4981 atacaaaaaa ggttgagaga actttctgga agaaatgttc tgactgttgc atttacaggt
     5041 tcaggaaaaa cagctgcaga ttatagaaag acttttaaaa gaaaattctt tttagtgcca
     5101 aataaaatat cagcagaaag tttttataga aaaaatattt ttcaaaataa aaatgattta
     5161 gatacaagaa gtagtaatga ttacattgga ttacttcatg gagatataaa cttatattca
     5221 gagaatgaag ataatggaga acatgatttt gtattgacat taagggatat agatttgagt
     5281 ataaactttt gtaaaccttg tgtaattgca acagttgacc agttattact tagtatgttt
     5341 aaatttccgg gatatgaaaa aatattcgca gccgtaaaaa atgcatctat aactgttgat
     5401 gaagtacatt tattggaacc aaagatgttt ttgattttga tatattttat gcagtttgca
     5461 tgcaagtact tagatgttga ttttcactta atgacagcta cacttccaaa atcatataaa
     5521 gagcaaatga taaacaaggg aattattttt caagaagaaa gtaatgaaaa tgttactgat
     5581 aaaggagaaa tagtttttat tgagagcaac aaggaagaag agatttgtga aggtaaagat
     5641 gtaaaggttt cttttataaa ggaaaaagaa atcaagtcaa tagttgaaga tgctttagaa
     5701 aataaacaaa aaattcttat aataaagaat actattgatt cagtcaatag aacatatgag
     5761 tttttaaaag aaaatttatc tgataagtat ggtggtgtag atatagatgt tttacatagt
     5821 agatttaagt tcaaagataa aaaagagaaa tatagtaaaa tattaaatgg agagggtgat
     5881 atttggatat caactcaatc agtagaaata tccttggatt tggatttcaa tataataata
     5941 agtgatttag caactatgga tagcttgata caaagaatgg gtagatgtaa tagaaataat
     6001 aaatatgaat atggaaactt ttatattcta cctagtgaag ataaaattta tgataacaaa
     6061 cttaagaaca caactaaaaa catcttaaag gacatattaa aaactaactc tatttttaca
     6121 atgggaatta gaaaaactat tttggatgat tactatgata atagtgtagt caaaaaatat
     6181 tttgaagaga attttataag ttgtgataaa gaaattaaga atatttatgg tataaataaa
     6241 gaaatttttg atggattgga tttaattttt aattttgaac cttacaaaaa tattgttgat
     6301 agtaagaagg acgcagctaa aatttttaga gatgttgatg ttagttacaa aattattctg
     6361 gaagaagatt tttataagga agatagggag ttacaacaag attcgattca agtatcaggg
     6421 tttatattta atagattata ttattatgga ttaataagca aagtagaagg atacatggta
     6481 ctaaaatcta gtagtaagtt tgagtataat agtactgtag gcttaatttt gaagtgagca
     6541 tatgtttaaa tataatatac ctttagacat tgaaaataca aaaagtacta ctgtaagtcc
     6601 actgtaaaaa ctatttttta aaaggaaaaa taagttgtga gatagagtgt ttacaagtgt
     6661 atatagataa taaatttgag gtttaagatt aactaagtgg gtttcaggta catcgcgaaa
     6721 ataattataa gttggagatt ttgtatgttt aagattaact aagtggattt caaatattga
     6781 tatagtagat ataatagaag ttttattatt aggtttaaaa ttaactaatc ggatttttaa
     6841 atattctgac tattatataa ggatattatt agcttgaatt taaaaccaat aatataaaac
     6901 aacaaaatat tatatttatg atataataaa agttatggaa aaacaggaat attactatag
     6961 agaagagagg taaacttatg aataattgga tagaaataac tataaaaaca acaacagaag
     7021 cagttgaacc tataacgaat atactatatg aacaaggagc tggaggagct gtaatagaag
     7081 acccaaaaga ctttttattc caaaagaaaa atgaactaga ctgggattat gttgaagaag
     7141 aagtatttaa aaagaatgaa gaagatgatg ttcttataaa aacttatgtt tctgaagaaa
     7201 aaaatgtgat ggaatttgtc gaaataataa agcaaaaagt tttaggatta aaagattttg
     7261 gtatagatat aggtgaagga agtgtatcat tggaccaagt aaatgaagct gattgggcaa
     7321 atgcatggaa agcatactac aaaccaacaa aagtaggaca aagggtagta gtaaagccta
     7381 catgggaaga ttatgctatg caagatggtg atttaatcat agagcttgac cctggaatgg
     7441 catttggtac aggtacacat gagactacta gtatgtgcat tagagaatta gaaaagtatg
     7501 tgaataaaga ttctaaagta tttgatattg gctgtggtag tggaatcctt gcaatagcag
     7561 cagccaaatt aggagcaaaa gaagttgtag ctgtagattt agatgaagtg gctgtaaaag
     7621 ttgcaaaaga aaatgttctt gaaaataaag ttgaaaaaag tgtatctgta atgcatggta
     7681 atttaactga tgtaataaaa gataaagcgg atgtaatagt tgcaaatata atagctgata
     7741 taataaagat acttgcaaaa gatgttcaaa actttatgaa agaagatgct atattcatat
     7801 cttcaggtat aattttggat aaagtagaag aagttaaaga aagcttaatt gaaaatggat
     7861 ttgaaatagt tgaagttcaa aaattaggtg aatggtcagc aatagtttct aagcttaaaa
     7921 aataagggat atggtgacgt ataatggata gattttttgt tgaaaaaaat aatataaatc
     7981 tacaagataa aacttgcact atagaaggtg aagatgtaaa acatatttca aaagttttaa
     8041 gatgcaaatt aggtgagaaa cttgaaatat gtgacaagaa taataatgag tatatttgtg
     8101 agataatgaa tatagataag agtatagtta atttagaaat tttagaaaaa gttgatataa
     8161 atagagaatc agaacttaaa gtaaggctat accagggact tccaaaagct ccaaaaatgg
     8221 aaatgatact tcaaaaatta actgaagtgg gtgtagaaga aataatatta gttcaaacta
     8281 agagaagtgt tgttaaagtt gatgacaaaa aagaagataa aaaatttgaa cgttgggaaa
     8341 gaataattta tgaagcagca aagcaaagta aaagaggtaa gataccaaaa ttaagaggtg
     8401 ttttaagttt taaagaagct ttagaagata tgaaaaaaaa taatgtaaat atttgccctt
     8461 atgaaaatga aagaacagtt tcaataaaac atgcactaaa aaaatgtgat tctaatatag
     8521 atagtgttgg aatttttata ggtccagaag gtggattttc tgaagaagaa atagaacaaa
     8581 tacaaaaaaa taattgtaat gttgtatcat tggggcctag aatattaaga actgagacag
     8641 cttctgttgt agcatcaaca atagctttgt atgaactaag tgatttagga ggagaaaaat
     8701 aaattgaaaa aagtagcttt ttatacactt gggtgtaaag ttaatcaata tgaaacagaa
     8761 gctatgctag aactatttga gaaagatggt tatgaacaag tcaatagtga agagtatgct
     8821 gatgtttatg ttataaatac atgtacagtt actcatatga gtgatagaaa atctcgtcaa
     8881 tatataagac gagttaaaaa gaagaatcca gatgcaataa tagccgttgt aggatgttat
     8941 tctcaggtat ctccggaaga aattttggat atagaagaag taaatttagt catgggtaca
     9001 aatgatagaa gaaagattgt tgaagaaatt aaaaatataa attcaagtaa gaaagttagt
     9061 acagttgatg acataatgaa agttaaagct tttgaagaaa tagaaatcag ccaaactaat
     9121 ggaaagacta gagcatttat gaaaatacaa gatggatgtg acagattttg tacttattgt
     9181 atcattcctt atgcccgagg acgcgttaga agtagagata ttgatagtat agtcgatgaa
     9241 gtgaaaaaat tggctaataa tggctataaa gaggttgtat taacaggaat acatgttgcc
     9301 tcttatggaa aagatttaaa ggatagggat attaaacttc ttgatgtaat aaaacaaata
     9361 aaccagatag aaaaaataga aagaattagg cttagttctg ttgaaccaat tttatttaca
     9421 gatgaatttg taaatgaagt tttaaagatg gataaagttt gtcctcatta ccatttatca
     9481 cttcaaagtg gatgtgatga aactctaaaa agaatgaata gacgatacac aactttagag
     9541 tacaaaacta ttgtagatag attgagaagt aaaatgccag atgtggcaat aactacagat
     9601 gtaatagtag gttttcctgg agagacaaat gaagagttta aaaaaacgta cgagttttta
     9661 aaagaaatag agctttcaca aatgcatatt tttaaatatt caccaagaaa aggaactcca
     9721 gctgcaacta tggaaaatca agttgaccct caaatgaaac actttagaag tgaacaactt
     9781 ttaaatttaa gtaaagtaaa ttttaataaa tttgcaacta aatttatcgg ccgtgagctg
     9841 gatgttttat ttgaacaaaa tatagaaggc aataaatatg agggattaac ttcaaattat
     9901 ataagagttg ttgttgaaag tgacaaaaat atacaaggtc agatattaaa agtgaaaata
     9961 aatgatgtaa aagatgaata tgtagaagga attttgctat aacatgtaga aattataata
    10021 tttataagat aggaggtgga aagaatggat tgtatttttt gtaaaatagc gaacggagaa
    10081 ataccttcaa ctaaagttta tgaagatgat agagtcttag cttttaatga cttgaaccca
    10141 gttgctccat atcatatatt agttgtacct aaaaaacatt atgatagttt aattgatatt
    10201 cctgataaag agatggacat tgtgtcacat attcatgttg ttattaataa aatagctaaa
    10261 gagaaaggtt ttgaccaaac tggattcaga gtcataaata attgtggaag tgatggaggg
    10321 caagaagtta aacatttgca ctatcatatt ttagctggta aaaagcttcc taattacgaa
    10381 gcgggacaaa actaaactaa attattgaaa aattgagttc cttcttgata aaaatacttg
    10441 ataaaagtaa aaatttagtt tataataaac aaagtgttag gttgtaatgc atattatgca
    10501 aaaagaacat agttacagtc ccagcataat tgcgctattg gagggaggga aaaagaaatg
    10561 tcagaagtaa gagttagaga gaatgaaaca ttagacagcg ctttaagaag attcaagcgt
    10621 caatgtgcaa tgtctggcat catgtcagaa gtgagaaaaa gagagcacta tgataagcca
    10681 agcgttaagc gtaagaaaaa agctgaagca gcaagaagaa aaaacgctaa gaaatagtag
    10741 taaataagaa taaagaggtg aaggggatgt cccttaagca aaagttacaa gaggatttga
    10801 aatcttcgat gaaaaacaaa gatacagtga gaaaatctgt agtaacttta ataagagctt
    10861 ctataaagca gtatgaggtt gataataggg tcgagcttga tgaagatgga attatcgatg
    10921 taatagcaaa acaattaaag caacgtagag atgcattagt tgaatttgaa aaagctggaa
    10981 gagaagactt aataaaagaa acagaaggtg aaattgaagt tttaaaagag tacctacctc
    11041 aacaattaag cgaagaagag ttagaagaaa ttgtaaaatc tactatatcc gaagtagggg
    11101 caacttctat gaaggatatg ggaaaaataa tgtcagttat ccaaccaaaa gttaaaggaa
    11161 gagctgacgg aaaacttata aataagttgg taaaacaaaa cttacaatag attgatatag
    11221 cctagagaga aatctctagg ctttttatag tttttgggaa taacgagtca tatttgagca
    11281 tatctataat caaagaaaat tttgaaagaa ggggaaaaat ttgattatat cagtaaaaga
    11341 gattaggaat tttttgataa acatatttat accactaata ataggttact taagtagcat
    11401 cttaacaatg attatcagtg gaacagatat ttcaacctat tatttacaac tcacaaagcc
    11461 aagctttgca ccaccagctt ttatctttcc tatagtttgg actatattgt acatattgat
    11521 gggtatatct tcatacctaa tattaagaaa gggttacaac ttacctaaag taagagatgc
    11581 aattttttat tatggattac aacttgtatt aaactttatt tggagtatac tattttttgg
    11641 atttggactt agatttacag ctttgataga tataataata atgattttgg tttctataat
    11701 aatgatttcg aagttttcta aaatagataa gagatctgga tacataaatt tgatttattt
    11761 aatgtggctt gtttatgcag gatttttgaa ctattttata tggtttatta ataaatagaa
    11821 tgtaaagatt ttgtaaatta ccctgtactt ttaataatat cataacaaac gaaaaataaa
    11881 tagactatta aaaataatca ttttcatata caataatatt aatttaaata caataaattt
    11941 ataaaaattg tatttaaaga ttaacatatt aaagtattag aattggaaag gcttgaaggg
    12001 agaggttttt atgcaaataa tagtatctgg gagacaaatg aaattaactg acggaataaa
    12061 aggatacgta gatggaaaat taagtaggtt agaaaaatac cttgatcctg aatcagaagt
    12121 gaaagtaaca gtaagtgcga aaaaggatag acaaaaagtg gaggttacaa taattcctat
    12181 taacggtcaa ataataagag cagaagacgt agaagatgac ttatatgcag caattgatat
    12241 tgtttgtgac aaattaagta gacaagttgt caaatataaa actaaagtta aagacaaagt
    12301 tcaaaataat aagagcataa gatttgaaaa tcttgacttt atagataata gcagtgaatt
    12361 tgatgattat gattatgatg atgaagaaga tgaaaatata gtaatagaaa gaagaaagaa
    12421 atttaatgta aaacctatga gttctgagga ggcgatactt caaatggaac tagtaggaca
    12481 caatttctat atgtttagaa accaagataa ttttgaaata aacatagtgt ataagcgtaa
    12541 agctggtgga tatggtttaa tagaacaaga ttaataaaaa ttgtatacgt ttagatttta
    12601 tataagaaaa aataaaaagg taatcatgtt gattttttat caatatgatt acctttttct
    12661 tgtttaaata atatattaga tattaggtat ttattatata aaaattttaa aactaatctg
    12721 ttacaacatc aataaagtgc ataaatacct atttcaagca taaaattaat taagggggta
    12781 atgcttatgt tagaaatatc cactgattta caagtaaatc aaccagttat tactgttaca
    12841 agtaatacgt ttattagtat agaaaattat ctatcaatat tagaatatga agttgattta
    12901 ataagaataa agactaaagt taaaacaatt aaaatatctg gggataaact ttcacttaag
    12961 tatattactg atagtgagat aggtataaaa gggattatct acaatgttga gtatgttgat
    13021 taggaggaga tacacttgat aagttttata aggggatact atgttatagt tgtggaagga
    13081 gtaggactcg aacaattttt aaatcatctt ataagaaatg ggataagtgt ttacaatgta
    13141 actagaataa aaaatactaa aatggagttt catatagata gacaagatat aaaagaattt
    13201 aagaatgttt atagaggaag taagtttgat ataaaggtaa agcaaaaaac tggggtccct
    13261 tttattatca aaagagttta taagcacaaa ggtatgtgga tttgtgctct tgtttcttta
    13321 tttttattga tgtctacatc acagtttgtg acagatgtat atatacaatc tcctgaaggt
    13381 ataaaaaaag aagctcttag aaatgagctt tataaggttg gagtaagacc aggagtctat
    13441 aagaagagta ttgatagaaa agaagttaga gaccatatga tgtcaaaatt taatgatgtg
    13501 gcatatttat ctatcaatgt aaaaggaaca aatatatttg ttactgttac aaaaaaggca
    13561 gaatccctaa aatctacaga ccaatcaaat tattgtaatg taattgcttt aaaaaatggt
    13621 ataatagaaa aggtaatccc tagaagtggt aaatcagtta ttaaatctgg agacatagtc
    13681 caaaagggag atgtcttatt aaatggagct aataccaagt caataccaga agtatgggca
    13741 agtacttttt atgagtccac aaaaaaggca agttatgtag atactgtaaa taaaaaaact
    13801 ggagaaaaga aaaatatata tacactaagt ttttatgata aagagtttac aatgagaaaa
    13861 aatataaaat ataaagatta tgttgttgaa aataaagaaa aaaaactatc aataggaaac
    13921 tatacttttc caataaagat aaaaacgagt actttttatg aaacaaaaaa ggttgaagta
    13981 aaaaggaata aagaagagtt aaaaaaagaa ttatctgaaa aagctttaaa agagttagaa
    14041 tacataatcc cagcttcggc tagaataata gatgtaaaac ataactttaa ggttaataaa
    14101 aatatgttag aatatttaat aacagttcaa actagtgaaa atattgctaa aatttatcct
    14161 cttagtaagt cagaagcaga aaggtttata aaggaagaaa gtaaaccaga tgaaggtgaa
    14221 gaggaagttc cttccaatcc agaaaaaaga ccattaaatg atataagaaa tgagtttgat
    14281 gaggataata aggataataa agaccaaaac aattctgatg aaaataatag taatcaaaat
    14341 agtaataata gtcaaaataa taatagcaat aacaactaat aaaattaaac ttaaaatctt
    14401 aggaggacta attcaattga taatacaaaa gaaatttaca atagcagatg gtaactttga
    14461 aagagagtta tttggcaact ttgatgaaaa tgtaaaatta atagagaaga ctttaaatat
    14521 agatgtaata cttagagaag gcaatataat tttaattgga gaagaaaaaa atgtagattc
    14581 tgcattaaaa cttatgaatg aattacacca aactgtatca aatggtaagc atttagataa
    14641 gcagagtatt tcttattcat tatctctttt gcttgaagga agtgaacaaa aaataaaaga
    14701 acttgagggg acaatcgtca taactcaaag gggaaaagct gttcaaccta aaactttagg
    14761 tcaaaaagaa tatataaaac ttatagaaaa taatgatata acttttggtg ttggacctgc
    14821 tggaacagga aagacttatc ttgcggttgc tatggcagta aaagctttta aaagagatga
    14881 agttagtaga attatactta caagaccagc tgtggaagca ggagagagtc taggtttttt
    14941 accaggagat ttaaaggaca aagttgaccc atatttaaga ccactatatg atgccttatt
    15001 tgaaatgttg ggtgctgata aatttaataa gtatttagaa agaggtacta ttgaggttgc
    15061 accacttgca tttatgagag gtagaacact tgataattca tttataatat tggatgaagc
    15121 tcaaaatact acttctgaac aaatgaaaat gtttttaact agacttggat ttggctctaa
    15181 agctgtagtt acaggagatg taactcaaac agatttacca caaaataaga agagtggtct
    15241 aatacaagct acagagattc ttaaaggtgt tccaggtatt gggtctataa tgctaacaga
    15301 tagagatgtt gtaagacatg agttggtaca aagaataata agagcttatg agaagcacga
    15361 caaaagagaa gaatttaaaa aagaagaacg taaaaaagcg aaaatgcagg aaaagacttt
    15421 caaaagaaag taaaagtgta gtaatataga tatatagcat tattaatgaa taaaaagtag
    15481 gtgtagtgaa tggatttgat actagatgat agacaagata aattagaagt aagtgaggag
    15541 ttaattgaaa agataaagga tataatcata gaatgtttag attatgaagg atatgatgat
    15601 aattatgaag tgagtctttc ttttgtagat aataaagaga ttcatgaatt aaatagagaa
    15661 tatagaggag ttgatagagt gactgatgtt ttatcatttc ctcttttaag tgatgatttt
    15721 gaggatgttg agttagaaga agaatcccta ggtgacatag tagtatctct tgaaagagcc
    15781 ttagaacaga gtatagagta taaccatagc tttgaaagag aagtatgttt tttaatatgt
    15841 catagtatgt ttcatctttt aggttatgac cacgatacag atgaaaatac aaaagaaatg
    15901 agagaaaaag aagaacacat attaaataag ttaaacataa caagggagta gtttttatga
    15961 aaccagaaaa aactcgtcaa ggtataatta aagcatttaa tgcagctata gaagggatat
    16021 tgtatacatt taaatttgaa agaaatatga aaatacatta tttaggttca gttgctgttc
    16081 ttataataag tttgtttttt aacttttcaa aattagaaat gataatgtta cttatgtcca
    16141 tatgcttagt tgtagttgct gaaatgttta atacagctat agaaaaagct gttgatttag
    16201 taactgatga atatcatgtt cttgctaaga tagcaaaaga tgttgcagct ggaggcgttc
    16261 tagtggcagc actaaattca gtagtagttg gatatatttt gttttatgat aaattgacag
    16321 atatatctgg aatattaatt tataaaataa gagaatcaga attacatata accttgatat
    16381 gtatattatt ggttcttata gctgttgtag ttgtaaaagc attgacttct acaggtactc
    16441 ctctaaaagg aggaatgcca agtggtcatg ctgccttagc atttgctata gcaactgcaa
    16501 ttacacttat gactgaaaga gttgttgcct ctactcttgc atatataatg gctgtattag
    16561 ttgcccaaag tagaattgaa ggaaaaatac ataccttttg ggagactatt gctggagctt
    16621 tgttaggtgt gttgatagct atattagtat ttcagcttgg aatgttctat aattaaaata
    16681 aatgtacata aaatatttaa aataaaacaa atctgtgcta attaaatttt tatgatataa
    16741 tgtagagtat taaataaaaa taacttttaa ttattgtata caggaggagt tgttgtggat
    16801 aacatagaat tgttaaggtt ggcagaagat gccagacaac attcttatgc tccttactca
    16861 ggttttagag taggtgcagc tcttttaact aagagtggta aagtttatac tggatgtaat
    16921 atagaatgtg catcattagg tggaacaaat tgtgcagaaa gaacagctat ttttaaagca
    16981 atatcagagg gtgataagga tatctataaa atagctatag caagtgataa ctcagaaaat
    17041 aatgagcaaa catatccatg tggaatatgc aggcaggtaa ttatagagtt tggttctgat
    17101 ataaaaataa taacgggata tactaaagga gaggtgtttg agcatagtat aaaggatttg
    17161 ttgccaaact atttctctgg aaatgattta aaataaattt aaaagtataa ataaatttaa
    17221 gtaattaaaa caagaagata aaaagttagg agacagttaa tgttcaaatc aggttttgtc
    17281 agtatagtgg gtagaccaaa tgtaggaaaa tctactttaa tgaataatgt agttggagaa
    17341 aaaatagcta taatgagtga taagcctcaa actacaagaa atacaataca agcagtatat
    17401 acagatgaag aaatgcagat agtattctta gatacaccag gtattcacaa accaaaaaat
    17461 aaattaggtg aatttatggt aaaggcagca acagaagctt ttaaaaatgt agatttaata
    17521 ttatttgtag ttgatgattc taaaaaaata ggtcctggag atagaaagat aatagaggat
    17581 ttaagaagtg ttaagacacc aataatttta gttgtaaaca aaatagacca actagaccaa
    17641 aaagatgagc tatttgatat aatcaaaatg tatgatagag aaggcatttt taaagaaata
    17701 gtaccaatat cagcattaaa aggaaagaat acggatacct taataaaagt tatacaaaat
    17761 tatttagaag aagggccaaa gtattttcca gattatatga ttacagacca gccagaaaga
    17821 gtattaatag cagaacttat aagagaaaaa gttcttcact atcttaatga tgaaatacct
    17881 catggtgtag ctgttgaaat tgaaaaaatg aaagcgagaa atgataagga aatagtagat
    17941 gtatctgctg taatatactg tgaaagagat tcacataagg gaataataat aggaaaaaat
    18001 ggtaggaaac taaaaggaat aggtaagtca gcaagacaag atatagaatt gcttttaggt
    18061 tcacaaataa atcttcaatt atgggttaaa gtcaaagaaa attggagaaa tttacaaaat
    18121 tatataaata attttggata taatgataaa taaaggtggg ttatatggat aggaaactgg
    18181 atgcttccca agatttatta tatgaagtaa aatcattaat tgataataat aaagtattgg
    18241 agttaagaga attaatagag gaatatcata taattgatat attcgatata atggaaaatt
    18301 tagaggaaga catgaaaatt caattatttg aagtacttcc tttagatatg gcatcttcta
    18361 tattagaaga aggtagtgta gagtttttta ttagtatttt atctaaactg gatgtagaac
    18421 actcgaaaaa tatacttgaa cttatgtctc ttggagatat ggcagacaag ttgagtgaac
    18481 ttgaagaaga agaaagagaa cacataataa atcttttgaa tcaagagaat gccgattatg
    18541 taaaagagtt actattctat gatgaagatt ctgctggtgg aacaatgacc actggatata
    18601 tttctataaa taaagatatg acagctcttg aggctataga ccatatgaga gaagaagctg
    18661 aagaagctga aactatttat tatatatatg tggttgatga tgaagaaaaa ctagttggag
    18721 tattgtcatt aagagaatta ataatagcta gagacgcaaa tatagtagaa gatttgatga
    18781 gtgaaaatat aatctctgtt tatgtagatg aagatagaga agaagcagta agattagtat
    18841 ctaaatacaa tttaatagca attccagtag tagacagaca agaaaaatta aaaggaataa
    18901 taacagtaga tgatataatt gatgtaatgg aagaagaagc aactgaagat atgtataaat
    18961 ttgcaggaag ttcagaacat gaaagagaag ttgctgaaaa agaaaaccct acattgagag
    19021 agcaaataat atcagcactt agaggacgat taccttggtt aattattacc ttagttggag
    19081 ggttgttggc ttcgttaata ctttctaact tagactatat aatgaatcct gtatatgctc
    19141 cattagtatt ttttatacct gtagtaattg gtatgggtgg aaatattgga actcaatcat
    19201 catctgttac agtaataaca ttatctaata aggatttaaa ttttagcaat gtggttagag
    19261 aaggtattgt aggtattatt acaggtcttt tgtgtagtat aataacaggt attgtaattt
    19321 actttgttat gagaaagtta gacattgtat taattgtttc aatatcatta tttataaata
    19381 tggttttagg tgcaacaata ggtgcgttta tgcctgtttt attaaaaaaa atggatgcag
    19441 acccatctac agtttcatca ccaattatat ctacagctct tgatataact ggtatagcag
    19501 tttactttat aataacaacg gcattattgt caaaaattgt ttaataactt tatgtatgat
    19561 ttatcctctt cttactaaaa atataagtat aaatgtaaga agggggtttt ttaaaaatga
    19621 acaatatcag ttgggaagtt tttaaaaaaa caggtagtat agatgcatat ttatatttct
    19681 gtgattgcaa aaacatacat gaagaaattg aagaagtaag ggagaaaaaa gaagacgatg
    19741 ataatcctga acacccaggg gatagtactt aaagctataa ggtataagga aagtgatata
    19801 atacttacac tatttacaag aaagcttgga aaagtatctg caatagcaaa aggagctaaa
    19861 aaaaataaga gctctttgct ttcatcatca cagttatttt cttatagcaa ttttacctta
    19921 aaaaaacaag gtaatatgta caaggttact caaagtgaaa taataaaaag tttttataat
    19981 atatcctatg atatagaggc attttcttat gctacatata ttacaaaatt agttgaaaat
    20041 tctatacttg aaaatcagac taataatagg ctatttatat tacttgcgca gactctgtat
    20101 ttgtatacac aggataatac agacaatagg tttataacag cagcattcga gttaaaattt
    20161 ttagactata taggatttaa acctatagta aataaatgca ttaattgtga atgtaaaatt
    20221 ttacaaaatt ctgtgtttaa tatctacgaa ggtggtatac tatgtagtaa gtgtagtaaa
    20281 ctttttgata ataatataaa attagactta acaactataa gtcttatgga atatgtatta
    20341 agaaatgata ttttaacatg tagtaaagct aaagtatcaa aatatataac acatgaactt
    20401 gagaatattc tagacaagta tttgaaagtt tatgttgata atattaattt taaatcatta
    20461 cacgtattgc aaagtgtaaa aaataataag ggagtggata atgatgagta gtaatataac
    20521 tattgaaatg gttgattcag tctttgaaag agttccagga gcaacttatg cagaagcaaa
    20581 agaagcatta gtaatgtgca atggagatgt aattgaggca gtaatatatt tagaaaataa
    20641 aaaaaatact tgtaattgta aagtaaaatc tgctaaagaa actatggaag aagtatttgg
    20701 caaagatgga gaaaatataa agtttcaatt aaaagagctt cttagaaaaa gtagtgtagt
    20761 gagaataata gttgaaaaag atggaaaaac tatgatgaat attcctttaa cagttggagt
    20821 tgttggatta gcttttgtac cattagctac tttagttgga ttatcagctg ctgttataag
    20881 taaatatcgt ataaaagttc aaaatgaaga agatggagaa attgtagatt taggtgaatt
    20941 gaatgaagaa aaacttcacg ttttgaagaa tatgataact aatgcagcaa aagatgtgaa
    21001 ggatgtagta gttgacaaaa aagaagatga caaagatgta actgctgacc ttatgaaaga
    21061 agaatctgaa aagaataaag aagacgaaga cgaaaataaa tagatataaa gtgactttta
    21121 aaaaatgaag agaagataat tataagaatt atcactaaca ttgacaaatt ttatagaatt
    21181 tgatattcta aaagttgtag aagaactatt aaaatagcct ttcgtgtaga cgagaggctt
    21241 tattgttata ggagggattt ttatgaattt tcaagagatg atactggcgt tgcaaaaata
    21301 ctggtcaaag caaggttgta tcatgatgca gccttatgat attgaaaaag gtgcaggaac
    21361 tatgaatcct aatacttttt taagatcatt aggacctgag ccttggcaag tatgctatgt
    21421 agagccttca agaagaccag cagatggaag atatggtgaa aacccaaaca gactatatca
    21481 acatcatcaa ttccaagtta tactaaaacc ttctccagat aatatacaag aattatattt
    21541 ggaaagttta aaagagattg gaattgaccc aagtgagcat gacataagat ttgttgaaga
    21601 taactgggaa gctgctacag ttggtgcttg gggacttgga tgggaagttt ggctagatgg
    21661 tatggaaata actcaattta cgtattttca acaagtaggt aatattgaat gtgagcttga
    21721 gacaggagaa attacatatg gattagaaag attagctatg tacattcaag aagttgatag
    21781 tgtttatgat ttaaaatgga atgataaaat aacttatggt gaagtattta ataaagctga
    21841 gtatgaaaac tcaatgtacg catttgaatt atgtgatgca gatatgttgt ttaatctgtt
    21901 tgatatatat gaaaaagaag cattaagact tatggaaaat ggccttgtaa taccttcata
    21961 tgactatgtg ttaaaatgct ctcatgcatt taatacattg gatgcaagag gtgcaatagg
    22021 agttagtcaa agagcttcat ttataggtag agttagaaat atggcaaaga ctgttgcaga
    22081 aacttttgta aaacaaagag aagaaatggg attccctctt ttaaaggacg gtgacaaata
    22141 atgaaaaatt atcttttatt tgaaatcggt gttgaagaaa tgccatcaag atttgtaggc
    22201 agtacactag aacaattaaa aaacaatctt agtaaattat ttgatgaaaa tagaatagct
    22261 tttgataaaa taaatgcgta tgggacacca agaagactta ctttagtagt cgaaggattg
    22321 agtgatagac aaaatgattt agaagaggaa ataaagggtc ctgctaagaa aattgctgtt
    22381 gatgcagaaa ataatttaac aaaaccagca ttaggattta taaaaagcaa aggattaagc
    22441 gaatctgaca tatattttaa acaagttgga aaagaagaat atatatttgc gactataaag
    22501 caagatggaa aagaaacttc agaagtacta aaagacatat taccagagac tataaaatct
    22561 gtagtattcc caaaaactat gcgttgggga ggaaaaaatc ttaaatttgc aagacctata
    22621 agatggatag ttgcattgtt aaatgataaa gtattagagt ttgatttaga aggaataata
    22681 tcttctaatg taacaaaagg tcatagattt ttaggtgaaa gtacaataga agttgattct
    22741 atagaagatt attttgaaaa attagaaaaa aattatatag ttttagacca aaacaagaga
    22801 aaagaaatga taagaaaaca atgtatagaa gtagcaaatt ctttaggtgg agaagttgaa
    22861 tttgatgaag atttacttga agaggtaact tatttagttg aatatccaac tgctttttat
    22921 ggagaatttg atgtggatta tataaaacta ccaaaagaag ttgttataac tcctatgaaa
    22981 caacatcaaa gatattttcc agtacttaaa gaaggaaaac tgttacctaa ctttatagct
    23041 gttagaaatg gtgattctta cagaatagac aatgttaaag ctggaaatga aaaagtttta
    23101 gaagcaagac ttgcagatgc tttatttttc tatagagaag atactaagag aaacttagaa
    23161 agctacatag aaaaattaaa aactgttgta ttccaagtaa aactaggaac tatttacgac
    23221 aaaactttaa gacttgaaaa attaagtgct gatatacttg atagtttagg tttagtagat
    23281 gaaaaggcag atactataag ggctgctaaa ttatctaagg cagatttagt tactggaatg
    23341 gtaggagaat ttgatgaatt acaaggtttt atgggaaaag aatacgcaaa agttggagga
    23401 gagaatgatg ttgtaagtga agctattttt gaacattatc ttccaagatt tgcaggagat
    23461 atacttccta agactaatgc aggagttgcc ttatcaatag ctgataagtt ggattcaata
    23521 gcaggtttct ttgctatagg aatacaacca acaggttctc aagacccata tgctttaaga
    23581 agacaagctt taggtatact tagtatactt atggacagaa aagttgctgt ggatataaaa
    23641 gtacttattg aacatgcttt agacaattat tctgaattag attttgataa agaagaagtt
    23701 gttgagcaaa taatgaattt cttcaatgag agagtaaaaa atctatttaa agatttagga
    23761 ataagatatg atgttgttga tgctgtttta agttctgata taaatgatgt ttctgatatg
    23821 catatgagag ctttagagct taataattgg ctagaaaaag atgaattagt ggagatgctt
    23881 actgcattta atagagtttc tactctagct caaaaagcag aaagtgataa aattgatgaa
    23941 tctttattaa aagaagatgc tgaaattaaa ctttacaatg aatttaaaca agtaaagata
    24001 aaagttaatg aattattaaa ggataagaaa tatggtattg ctttagatga atttgcatct
    24061 ttaagaccag tagttgataa tttatttgat acagttatgg ttatggataa tgatgaagct
    24121 attaagaaca atagacttgc tttacttaaa caagtatatg atactatgct agaaatttgt
    24181 gatttatcta aaattgttta taaataaaat ttaaactatt tataagaaat aaggctgtct
    24241 caaaatagag atagtcttat ttttcattgt tgagatgatg aaaatatatt tgatttcata
    24301 gaaaaagagt gtctcataaa ctaaaaagtt cattttgaga cacccatgtt ttttgtaagt
    24361 aaatataata aactatattt tatacttatc agttattttg tttaaaatat tatcatttga
    24421 atttacccat tcataaaaat tgcctgatga tgcgtccgat attttataaa gcatttcagg
    24481 aatatcacta gctttaaaat ctccaagtga ttttcccaat ctagtcttac ctatttcaaa
    24541 actgcctcca acaaataatt caaatgtatc tgtagatata ccatcaacat tcttcttttt
    24601 accacataaa cctatgctac caacttgatg aacaccacat gaatttaaac atcctgatat
    24661 atataactta ggtgctttat ttaaaatttc ttcattattt tgtaatctaa aataatctat
    24721 gatttcatgt aacattttct gactattttg tatgcccatt tgacaaattg gaacacctat
    24781 gcaagatata ctttgttcta attgagaatt acaactaata gttttactta tttctaatat
    24841 tctttttgct tcatttccat ttaaatttaa aatatataat ccctctgtca ttcctagtct
    24901 aatcatagga ttttcaacat tatctaattc tttaagtaaa gtacttaaat cctttgttaa
    24961 caaaatacct ccaactggat gtatatatac agtgtataaa ccatcctgtt tttgaggtat
    25021 tagtctagaa tcttgtatat ctattttaac tccaggttta gagtaatcta tttcttcaat
    25081 attgagttca agactaccat tttctttttc taatttatag tattctttaa atttttctaa
    25141 gaatagttct tcaccaagtg attcgaccat atatctaacc ctagctctgt ttttattttc
    25201 ataatttcca taatccataa agaatttaat cataccttca acacaatata aggcatcttt
    25261 cggctttata agttcatcaa gttctaaagc aactttgggt tgtttaccta gacctccacc
    25321 taagaaaact ctgaaatatg gttcatcatt tttcaaagta gcaacaaatc ctaagtcttg
    25381 tatactacaa tgagcagtat ctgtgtaaca attagaataa gaaactttta attttctagg
    25441 taaatggtat gtagtagcat ttttgataaa atatttatca gtagcaactg catatggagt
    25501 aacatcaaag acttcactag ggtcaactcc agataatgga gataatccga catttctagg
    25561 ataattacca ccaccacctc tagtgaatat atcatttttt attccttctt ccatgatatc
    25621 aacaattgaa tttatagata aatcgtgcaa ttgtatagct tgtctagttg ttaagtgtat
    25681 cttatctaag ttgtgtttaa tagctatttc atagatttta ttaagttgat actgagataa
    25741 aactccagca gatactctaa gtcttatcat aaaagatttt ttgtctcttt gagcataaac
    25801 accatatcca ccagaaaatc ccttatattg cattttattt atttctccat ttaaaaattt
    25861 tgaagaatat tctttaaaat taggtagttc atttaacaat atctttttaa aattatccat
    25921 tatttcacct cattgttagt cttgtataaa aatatatcta ttgtataata gatatatata
    25981 ttattgtcta gtcaggataa aaatataaca gaataattct attaaaagta agaataataa
    26041 ggacatagtg agtattttgt ttttatttta caaattaagt ataatatttt tgttttataa
    26101 aatataatta agataaatag ataattttgt aattttaata tataatattt agtagagtgt
    26161 gggagtagca cttaatattt gcaggaggag tttttgtata tgggagaaat aataaatgca
    26221 ttagatagag ccctagacat aattttgctg ttatatcatg aaaagagaga aatgggaata
    26281 actgaaataa gtaaggctat gggagtatat aaaagtactg tacatagaac tttggttact
    26341 cttgaaaaca aaggttttgt tatacaaaat gcagagaatg gtaagtattg gctaggaata
    26401 aatttatatg caataggtat ggttgtggga gaaaaaatgt cattgactga aatagtaaaa
    26461 ccatatacga aaaaattaaa tcaagaattt aacgaagtag ttaatgtatc tatattagaa
    26521 gaaagagctc aagattctcc aagaagtatc ataatacata aagagtatgg ttcaaatcaa
    26581 ttactttcag taaatccatc cgttggttca agtagtgaat gttattgttc agcagttggt
    26641 aaatgtctta tggcatttaa tgattctata gattttgaaa aatatagaaa aacaccaata
    26701 cacaagtata cagaacatac aattgataat tgggatgata tgatgctttt tttagcaaaa
    26761 ataaaagaac aaggatatgc tatagatgat gaagaactag aacatggact aacttgtatt
    26821 ggagcaccta tcttagataa aaataataaa gctattgcag ccataagttt gtcaggtcca
    26881 actataagaa tgagagaagg ggactttgag tataagatta aaagagttat agaaactgca
    26941 aaaagtatat ctgaactttt tagatgattt ttaatctcga taataaaaat aggctatatt
    27001 taaattgtta tttgtaaata tcagtttaaa tgtagcctat ttttctttaa tctaaaatta
    27061 aatattctta aaatttaaaa tgttattgaa atatataagc gaatgtgtta tcttttaatt
    27121 aagcaataaa aatagaacaa ggttctatac tatagaacaa aattgatgca tatatagtat
    27181 ttatacaaaa tataaatatt atatatttaa aattaagtaa aaatattaca taaaggggag
    27241 atttgagaaa tggaaaaagt caagttgagt gttgaaagat gtaagggatg ctatctttgt
    27301 attgaagctt gtaaaaaaga tgcaataagt ctttctgact acacaaataa aaaaggcttt
    27361 gtaactatta aagtcgacga agaaaaatgt attcaatgtg gcgcttgtta tacaatgtgc
    27421 cctgatttag tatttgagat attataaatt atttttatta gaggggagaa aaggtatgca
    27481 aagtttatta attaaaggaa atgaagcttt agcagaaggt gctatacgtg gtggctgcag
    27541 attttatgca ggttatccaa taacaccaca atcagaaata ttagaaatga tggcatggag
    27601 acaaaaagaa gttggaggag tatttatcca aggtgaatca gaaatagcca gtgtaaatat
    27661 ggttatggga gcaagtgcac ttggagcaag agctatgact tcttcatctg gtccaggatt
    27721 ttcattaaaa caagaaggta ttgcatattg ggtatcagct ggtttgccag cagttgtagc
    27781 atgtgtgcaa agatatggta taggtgatgg atttatagga gctggtcaag ataattattg
    27841 gcaagcagta aaaggcggtg gaaatggaga ttatcattta atcgtgttag cacctaatag
    27901 tgttcaagaa aatatggata tggcatatga atcttttgaa ttggctgaaa aatatatgca
    27961 tccagtactt atactttctg atgctacaat aggtcaaatg gtagaacctt gtatagcacc
    28021 accaatgaaa gaatacgata tggataaagc tccatgggct gcaaaaggaa cacctgtagg
    28081 agaaaaaaat aaagtaataa ctgatataac atattttgaa gaccaagata catggcaaaa
    28141 agggtatata gaaaaaatga gaaaagttca agaagatgaa caaagatggg aagagataga
    28201 aatagaagat gctgaagtgg tatttgtggc atacggtatt tcatcaagaa tagcagaagg
    28261 tgcagttaga gatgctagag caaaaggaat gaaattaggt ctaataagac caaaaacatt
    28321 gtggccttac ccaagaaaag catttaagaa attaagtgat tcattgaagg gacttgtaac
    28381 agttgaaatg aacatgatgg cacagatgaa ggaagatgta attatagcat gtgataatag
    28441 atttccagta tattcagtag caacagctca atatatggca gaagtaaatc aattaataga
    28501 ttttgctcaa cagattatag atggaaaagc taagcaagag gaggtatttt aattatggct
    28561 aaaaaagcac cagcaataat gatgtgtgaa aatatgttct gtggaggctg tggacactct
    28621 atagtcgata gaattattgg agaagtatta acagaaatgg atatatgtca agatacaatt
    28681 atatgttcag atataggttg taatcatatg tttcaattta gtatgaatgt agatgttgtc
    28741 gttccacctc atggtaaaat gggagcagca atggcagcta tgaaaagagt tagaccagat
    28801 aaatatatat ttacaatagc tggagatggt ggagcttatg caatagggct tggagaaact
    28861 atgtcagctg ctactagaaa tgataatgta atatttcttg ttatgaacaa taatatgttt
    28921 gcaatgacag gagggcaaat ggctccaact actatagaag gtcaatatac agcttctact
    28981 ccagcgggta gaaattacgc agatcatggt tcaacatttg atgttgtaaa agttatggga
    29041 aatttagata tagcatatct agcaagagga acaataacta accctaaaga aataaataca
    29101 acaaaaagat acatcaaaaa agctctagaa aaacaaaaag aaaagaaagg tttttgctta
    29161 atagaagtgc ttgtaccatg tccaacaaac tggggattaa agccagtaga tgctatgaag
    29221 agaattgaaa atgaacttgt tccaaagtat ccacttggag aatttattga ttagggagga
    29281 aataatatgt tgaatgaaat tatatgtgct ggttttggag gacagggagt tcttacagca
    29341 ggaaaaatta taatgtatgt tgcatataag gcagaaaaga aaattacatg gttcccatct
    29401 tatggaaatg agatgagggg aggaagtgca aattgcaatg ttgttgtaag tgatgaaaaa
    29461 attgcaaatc cttgtgtaaa aaatccagat atactattgg gaatgaacag cgtatcagta
    29521 gataagtatg aaagtttaat gaaaaaaaat ggttatatgt ttgtcaatga atcactagta
    29581 agtaaggata gaaagtacag agaagacatt aatgttataa aagtacctgt tactgaaata
    29641 gcacaaaatt taggcaatga aaaagctgct aacatttgca tgttaggtgc tatggtaaag
    29701 agtttggatt tatttacaaa agaagaattt gaaagtggta tgtgtgaata ctttgaaaat
    29761 caaggcaaag gtaaattcaa ttctaaaaat gtagaagcat ttgaagctgg atacgactac
    29821 gaatgaatat tttaaatatt taaaataatt attgaaatac ttatttttat atgttagtat
    29881 ttaattaata aaatatagaa cgtcgttctg gacaatggga cgaagtcttt aggttggaat
    29941 acaagaacga cgttctgtat agtagaacta gggggtgtta aattgaaagt tttagtaata
    30001 aatcctggtg gtacatccac aaaaatttct gttttccaag atgaaaatga aattttgaaa
    30061 aaaaatataa cccatacaag agaagattta aaaaattttt ctaaagtatt tgatgaatat
    30121 gattatagaa aacaactgat agtagatatt ttaagtagtg agaatcatag tataaattct
    30181 tttgacgcag tagttggtcg tggtgggctt atgaaggcta taaaaggtgg tacctatact
    30241 gtgagtgaag aaatgattga ggatatgaga aatgaaataa atggggaaca tgcatcaaat
    30301 ttaggtgcat tattagctaa aactatagct gatgaaatag gagtacaatc atttgtagta
    30361 gacccagttt ctgtagatga atttgatgat gtatcgagga ttacaggaat atcagacata
    30421 gaaaaagcaa gttggttaca tgcattaaac cataaagctg tttcaagaaa aattgcacaa
    30481 aaactagggg gaaaatacga ggattataac tttattgtag ctcatttagg ttcaggaata
    30541 tcgattgtag cacataaaca aggcaaaatg attgatggaa gtggtggtag gtctgatggt
    30601 ccattttctc cagaaaggag tggtggatta cttgcatatc ctcttataga attgtgttac
    30661 tccggaaaat atactcatga tgaaatggtc gcaaaagtaa gttcgattgg gggaatgtac
    30721 gattatttag gaacaaagga catgatagaa attgaaaaca gaatcaagga gggtgatgaa
    30781 aaggcaaaac ttataatgga tgcatttatt tatcaagtag caaaagaaat ttctatgtat
    30841 ggagcttcac tttcagggaa tgtagataga gttatcttga caggagggat atctcactca
    30901 gaaatggtag tcaaaggagt agcagaaaaa gtttcatatc tagctccaat agagatagta
    30961 ccaggagaga tggaaatgga ggcacttgct ctaggggcac ttagagtact taatggagaa
    31021 gaaatagcaa aaacttatta atatttagga gaaagagtat gttaaaagat tttaataatt
    31081 tattagaaga ggctaaaaag cttgaacctg ttattgtaag tgttgctgtt gcagaagaca
    31141 aagaagtatt aaaggctgtg aaatcagcaa cagatttaaa cattataaag ccaattcttg
    31201 taggagacaa ggataaaata gaaagtatat caagagaaat tggattaaaa ggtcatgaaa
    31261 taataggttg tcaaaatccc gatgaatcag ttaaaaaagc tgtagagata gttaaaaata
    31321 aggaagctaa agttcttatg aagggattag taaatacaag tgtctatatg agagctatat
    31381 taaataaaga aacgggtctt agaacaggaa gattattaag tttgcttgca gtatatgaat
    31441 tacctcaata tcataaactt ctttattgta ctgatagtgg aataaatgta tcgccaaact
    31501 taaatcaaaa aaaagatata atgacaaatg cacttcttac tatgaaaagt ataggtatgg
    31561 aaaatcctaa aactgcgata cttactgcaa atgaaacggt agaccctaag atacaatcta
    31621 cagtggatgc caacctactt gtagagatgg aaaaatcggg agaaatacca aagtgtatag
    31681 tggaaggtcc aatagcattt gatgtagcat ttgatgcaca tgcagcagaa cataaaggta
    31741 ttaaaagtaa tattgctggt gatgttgatt tattagtatt tccaaatata gaagcaggga
    31801 atatattagg aaagtcatgg cttagattta ataaagctaa gtgggcaggt atagttttag
    31861 gagcaacaaa tccgataatt ttaggttcac gttctgacac agctgaaata aaaataaatt
    31921 caatagcatt agcgtgccta gcatcacaat aagaaattat tattaaggag gaaaaagtat
    31981 tatgaaatat gcaaaaagaa tggatatggt aaaagcttca gctataagag attctcaaaa
    32041 gaaaatagct cagaaggtag caagtggtgg aactgttata tcttttgctg cagggcttcc
    32101 tgacccaaat ttatttccaa tagaagagat aagtgaaata actcaaaaag tacttaatga
    32161 aaggggaaaa tttgccctag catatggacc tactaaaggt gaagatgagt tgttagatct
    32221 attagtaaaa agaatgaaag aagaagaaaa tattgataca aaagcagaaa atattataat
    32281 aactactggg tcacaacaag ggatagcatt aagtgctatg attttattag aaaagggaga
    32341 tgtagtagtt acagaaaatc caagttactt aggtgcaata aatgcattta gaccttatga
    32401 atgtgatttt ttaggagtag atacagatga agaaggaatg gtaacagatg atttagacaa
    32461 attattatct gaaaattcta acataaaggt gatatatgta ataccaacat ttcaaaaccc
    32521 tacaggaaaa acatggtctt tacaaagaag aaaagatttt atggaagtcg tgaataaata
    32581 tgatgtcata gtaatagagg ataatcctta tggagaaata agatttacac cagaacctct
    32641 acctacactt aaatctcttg atactaaaga taaagtattg taccttgggt cattttcaaa
    32701 ggtattatgt ccaggattta gagttgcttg gatgtgtggt aatgtagaca tcatagataa
    32761 ggcagaactt ttaaaacaag gtatagattt acagtcaaat caattttcgc aagtacaagt
    32821 aatagaattt ttaaagaaat acaacttaaa tgaacatata gaaaaaataa gagcagagta
    32881 taagaaaaga tgtctgctaa tgttagatgc tatgaagaaa catttcccag aggaagctaa
    32941 atatacagaa ccagatggag gaatgtttat atgggttgaa cttcctgaaa gtataaatgt
    33001 agatgattta ttagataaag ccattgatgc tggagttgct tatgtttctg gagagtcctt
    33061 ctttgcaaat aatggtccaa aaaatactat gagacttaac tatacaacaa tgtcagaaga
    33121 acaaatcgtt aaaggtgttg agatacttgc agaagttata aaaaaagaac tcaattatgc
    33181 ttgtgaataa atataaaaag tgagggttta aatatgggag aaaaagaaaa cggtataaag
    33241 tttggaaagt taatgtttca acttgttatt atagcaatat cttgggaatt agtttatatt
    33301 ataccattta tccaatatac attatatgat ccaatcctaa aagcattaga atgtacaaac
    33361 actcaactag gttttctact tacaatttat gggttaggta atatatttgg agctcctata
    33421 ggaggctggt tagcagatag atttgattat agaaagataa ttttaggttc tgtatttctt
    33481 aatggagtag tatcattttt atttgcattt aatatgaact atccatttgc agttgctact
    33541 tgggtaggtt gtgcaatcac atcattagtt atgaactatc cttctatggt aaaaatactt
    33601 agagttatag gaaaagataa tcaaggtaag gtttatggat ttaatgaagc tatggttgga
    33661 gtttctggag tagtaatggg agcaatattc ttatatattt atacatgctt tgcaactcct
    33721 acattgggta tgagatgggt aatgatatct ttaggaatat tatcaatagc aatgtgtcca
    33781 gtattatggt ttgtaattaa agatgtagat attaaagaag aaagagaaga agcaggtgaa
    33841 aaaatgtctg ctggtgattt tataacagta ctaaaatcac caaatacttg gctagtagga
    33901 ataagtatat tctgtgtata ttcattcaca gttacaatgt cttattttac accatatata
    33961 acatctgtac ttggaggttc tgtagcatta tctggtgcat tagcaataat aagacagcat
    34021 ggtctaaaat tatttggagc accatttgga ggatattgtg cagacaaatt aaaatctcct
    34081 acaaaagtac ttttaccaat atatgtattt ggtatagcag taatagtttt attcctagta
    34141 ttacctgcat caactccaat gactatattc atagctttaa catttgtagt aggtatatta
    34201 ggttacatgg gaaaaggtat atattatgca gtacaagatg aagtaaaagt tccagttaaa
    34261 tattcagcaa ctactatagg tatagctgca gctttaggat tttcaccaga tgtattccaa
    34321 tttgcattaa taggacactg gatagatact tatggcaata aaggttacac atatacattt
    34381 atattccaaa tagcaattct agttattgga atactatctt gtttatatat attaaaggtt
    34441 aaaaagagaa gagcaagtaa attagaagct taaatattca atttggtaat taagtatata
    34501 taaatacaat acttattttg atgaatgtga ttgtataaat aacataaaaa atatcaatag
    34561 attaagtttt ataataatgt ttagagaata aagtataaaa atcaaaatgt tatcaagttt
    34621 tctaggtttt aacataaagt tacccctgaa ggtgtaaaat tgagtggcaa ttattatcta
    34681 ttttcttacc tacataaacc tatttaattt aaattctaag agattttaaa aatagatggt
    34741 agttgtactc aattttaaac ctcataataa atacgaaaat ttaaaatcta actagatact
    34801 atttaat
//
LOCUS       DAEORZ010000030        33996 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEORZ010000030 DAEORZ010000000
VERSION     DAEORZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33996
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.030"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(237..806)
                     /gene="yfcE"
                     /locus_tag="KRH39_001309"
     CDS             complement(237..806)
                     /gene="yfcE"
                     /locus_tag="KRH39_001309"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBG8511227.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(831..1631)
                     /locus_tag="KRH39_001310"
     CDS             complement(831..1631)
                     /locus_tag="KRH39_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBG8511228.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            1766..2626
                     /locus_tag="KRH39_001311"
     CDS             1766..2626
                     /locus_tag="KRH39_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8511229.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            2626..3312
                     /locus_tag="KRH39_001312"
     CDS             2626..3312
                     /locus_tag="KRH39_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG8511230.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            3364..3906
                     /locus_tag="KRH39_001313"
     CDS             3364..3906
                     /locus_tag="KRH39_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511231.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            3985..4452
                     /locus_tag="KRH39_001314"
     CDS             3985..4452
                     /locus_tag="KRH39_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBG8511232.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            5161..6297
                     /locus_tag="KRH39_001315"
     CDS             5161..6297
                     /locus_tag="KRH39_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBG8511233.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            6356..7138
                     /locus_tag="KRH39_001316"
     CDS             6356..7138
                     /locus_tag="KRH39_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG8511234.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            7157..8167
                     /locus_tag="KRH39_001317"
     CDS             7157..8167
                     /locus_tag="KRH39_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG8511235.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            8186..8983
                     /locus_tag="KRH39_001318"
     CDS             8186..8983
                     /locus_tag="KRH39_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG8511236.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            9081..9926
                     /locus_tag="KRH39_001319"
     CDS             9081..9926
                     /locus_tag="KRH39_001319"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBG8511237.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            10013..11188
                     /locus_tag="KRH39_001320"
     CDS             10013..11188
                     /locus_tag="KRH39_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG8511238.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            11384..12286
                     /locus_tag="KRH39_001321"
     CDS             11384..12286
                     /locus_tag="KRH39_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG8511239.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEDLINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            12300..13304
                     /locus_tag="KRH39_001322"
     CDS             12300..13304
                     /locus_tag="KRH39_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG8511240.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(13448..13675)
                     /gene="acpP"
                     /locus_tag="KRH39_001323"
     CDS             complement(13448..13675)
                     /gene="acpP"
                     /locus_tag="KRH39_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG8511241.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            13847..14272
                     /locus_tag="KRH39_001324"
     CDS             13847..14272
                     /locus_tag="KRH39_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511242.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            14320..14526
                     /locus_tag="KRH39_001325"
     CDS             14320..14526
                     /locus_tag="KRH39_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511243.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            14708..14887
                     /locus_tag="KRH39_001326"
     CDS             14708..14887
                     /locus_tag="KRH39_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511244.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            14921..15121
                     /locus_tag="KRH39_001327"
     CDS             14921..15121
                     /locus_tag="KRH39_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511245.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            15374..16390
                     /gene="ccpA"
                     /locus_tag="KRH39_001328"
     CDS             15374..16390
                     /gene="ccpA"
                     /locus_tag="KRH39_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBG8511246.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(16513..16911)
                     /locus_tag="KRH39_001329"
     CDS             complement(16513..16911)
                     /locus_tag="KRH39_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511247.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            17123..18028
                     /locus_tag="KRH39_001330"
     CDS             17123..18028
                     /locus_tag="KRH39_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511248.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGETEDNEDELVEDKNKGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            18143..18323
                     /gene="ssrS"
                     /locus_tag="KRH39_001331"
     ncRNA           18143..18323
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KRH39_001331"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            18506..19723
                     /gene="cdeC"
                     /locus_tag="KRH39_001332"
     CDS             18506..19723
                     /gene="cdeC"
                     /locus_tag="KRH39_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBG8511249.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(19823..21145)
                     /locus_tag="KRH39_001333"
     CDS             complement(19823..21145)
                     /locus_tag="KRH39_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG8511250.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(21196..21990)
                     /locus_tag="KRH39_001334"
     CDS             complement(21196..21990)
                     /locus_tag="KRH39_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG8511251.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            22100..23545
                     /locus_tag="KRH39_001335"
     CDS             22100..23545
                     /locus_tag="KRH39_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG8511252.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            23574..23783
                     /locus_tag="KRH39_001336"
     CDS             23574..23783
                     /locus_tag="KRH39_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBG8511253.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            23803..25197
                     /locus_tag="KRH39_001337"
     CDS             23803..25197
                     /locus_tag="KRH39_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG8511254.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            25437..26189
                     /locus_tag="KRH39_001338"
     CDS             25437..26189
                     /locus_tag="KRH39_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8511255.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            26215..26628
                     /locus_tag="KRH39_001339"
     CDS             26215..26628
                     /locus_tag="KRH39_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8511256.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            26632..27375
                     /locus_tag="KRH39_001340"
     CDS             26632..27375
                     /locus_tag="KRH39_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBG8511257.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            27375..27845
                     /locus_tag="KRH39_001341"
     CDS             27375..27845
                     /locus_tag="KRH39_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8511258.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            27862..28620
                     /locus_tag="KRH39_001342"
     CDS             27862..28620
                     /locus_tag="KRH39_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8511259.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            28604..29446
                     /locus_tag="KRH39_001343"
     CDS             28604..29446
                     /locus_tag="KRH39_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG8511260.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(29606..30511)
                     /locus_tag="KRH39_001344"
     CDS             complement(29606..30511)
                     /locus_tag="KRH39_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8511261.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFIKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            30873..31607
                     /locus_tag="KRH39_001345"
     CDS             30873..31607
                     /locus_tag="KRH39_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511262.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVANKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            31818..32999
                     /locus_tag="KRH39_001346"
     CDS             31818..32999
                     /locus_tag="KRH39_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBG8511263.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            32983..33555
                     /gene="mobB"
                     /locus_tag="KRH39_001347"
     CDS             32983..33555
                     /gene="mobB"
                     /locus_tag="KRH39_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBG8511264.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            33674..33949
                     /locus_tag="KRH39_001348"
     CDS             33674..33949
                     /locus_tag="KRH39_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8511265.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
BASE COUNT        13388 a         3676 c         6009 g        10923 t
ORIGIN      
        1 aatatataat gaaataagta aactaaatta tactccaata taatatataa tagaaacaag
       61 taaactaagt tatatttcaa tatagcatat aataaaacaa gtaaaccaga ttctatttaa
      121 ttagaagttt aaggtattgt acttaaactt ctaattgaga tagaaataaa atggtttact
      181 tgtctttatt atgaatttaa taaattaaag cttattttat ttttattaaa aatttactaa
      241 tcattgatat ttattatatt tccagtattg atgtcaatta agtttatatc aagttcaagc
      301 tcatcctcat catcagtttt tatgatatct ataattgcga cagaatggct tccatctttt
      361 gggatagaag tacttcctgg atttattact attaaattat catccatgta tagttttttt
      421 acatgagtat gtcctaaaac taatatgtca ccacccattt tctttgcttt gtcaataatt
      481 tcttcttctg attctatata accatgattc aatataattc taatttcacc aaattggctc
      541 atgacataag gactctgaat aggatgttct ataaccatct ggtcaacatc tgcatcacag
      601 ttccctttta caatcacaat gttttctaat ttatttaatg tctcaataaa ttttttagga
      661 ttatatcctt ctggaatatc atttctaggt ccatgatata agacatctcc accatgtaat
      721 aaaacatcac aatcagataa tacatttaac gctttttcaa agtaaagtaa gctaccatga
      781 gtatcactca ttattcctat tttcatatta aaaatcctcc taagtacagt ctataaaaat
      841 ttttcagata ctctatgcca aaactcatag tttgacattc taagtaattt tatttttgca
      901 tcagatatac taacttttat attttcgatt tgtctaaatc tgtgctcaac tccatcaacg
      961 acaatcaata cagaatcttc aaatctatat tctggtgcaa tctttataac agattcattt
     1021 gaacatatta tacttgatgt aaagcaccta tatgcattgg tatttatcgg atgtaaaggt
     1081 gtaatctgca taagttcaag atttatatca acaatactac ctccagcaga atagttatag
     1141 gctgttgaac ctgtggaagt agaaataatc ataccatctc cactaaaatt ttgtatatgt
     1201 ttattatcaa ggcttaaatt taggtgtata gtcctagact tgtctcctct tataacaacc
     1261 tcatttacag caagcatatt agaaccacta gtagttgtat atacttccgc attaagaagt
     1321 gacatttctt gaataatata atcttttttt gtataagctt caataaagct atcaatctta
     1381 tctggtaaaa tatcaggaaa aaaccccaga tgacctgtat ttattcccat tatgggaatc
     1441 tcaggaaaat cgaaatctct aactgttctt aaaaaagagc catcaccacc aattgatata
     1501 ataagctctg tgtctggata aatatctgaa tacacttcaa agccagcatt gataagtttt
     1561 ctagttaaga gttctttagt ctcaagggac ttatttaatt ggtttgtatt tatagttata
     1621 attcttttca tatattcacc tcttcatact ttgtattata acatagtttt ataattctta
     1681 atttataaat ttataaatga cctttgtagt gaatgtgtta aaatatgtat aggtgggtat
     1741 aaaaaaatgc ataagaggtg aagatgtgtt tagcataagg aatatcacaa aaaaattagg
     1801 aaaattcaag ttaaataata taaacttaga gttaaaggaa ggggatattg ttgggattat
     1861 aggaccaaat ggttcgggaa aaacgacttt aataaaaatt ataatgggga ttattgatgc
     1921 agatgaaggt gaaattgaac tttgtaatga aactattgaa aattctccaa taagctttaa
     1981 aaacaatatt ggttttgtat atgattcttt acaattttat ccgcatttaa aggttaagga
     2041 atttagaaaa atagtttctt tattttataa gaattttgat agagaaaggt ttgatgaata
     2101 ccttaataag tttgatatag aagaaaatat gcatattgaa aatttatcta aaggtcaatc
     2161 tgaaaagctt atgttatcaa gtgcacttag tcacaatgct aagttattga ttttggatga
     2221 accaactgct gggatagacc caattgtaag aactgaaata atgcagtact tacaagattt
     2281 tgtgaaaaat ggaagtagta gtgtaataat ttctactcat aatactgata atttaattaa
     2341 aattgctgat tatcttgttt ttataaatag aggaaatcag atttttactg ttaagaaaga
     2401 attaatagaa caagaatata agataattag ggcaaataaa gcagagttag aagcaattaa
     2461 agaaagcata gttggtgtga aagagtataa atattataat gaggcacttg ttaaagttgg
     2521 agatagcttg aaagtaaaaa gcctattgat agaaattgat aagcataagg tcaaaaatcc
     2581 tactatagaa gaactaatgt attattatgt aaatgaggtg agatgatgtt caatttaata
     2641 agaaaagatt taataatagg gattagttca gatggtatcc gtaatttaaa gtacatattg
     2701 ctattttttg tgttttattt tttccttaat tcaatttcat attatacagt aagtattgtt
     2761 gttagttatt tgatatttat aaatacgttt gagtgtgatt atgaaaatga ttcaaggatt
     2821 tttataagga gtatgccagt tagtatagaa gatatagttt actcaaaata tttattaggt
     2881 gtaggattaa taatttctgt aacaataatt gtgtccttac taagtaaatt gacatcacta
     2941 gtgttcttta gaaatatggt tttaaatgat gtattttttt ctgtaaatat atttttagca
     3001 attttgacga ttttactgcc tttaattttt aagtttggat atggaaaaat gaaagtttgt
     3061 ggttttatag taagtatttt aatttacttt gtatatggaa gtcttttgaa gatgattagt
     3121 atgattgtat atcaagtaaa gcattttaat tatagtaaag ttggaggagt atatctttca
     3181 aattatgtaa ctgatatagc taatactaag tatataaatt tatatagtat gacattttta
     3241 acaataataa tttttataat atcaatgtat ttttctatta agatttcaaa aaagaataag
     3301 tttaattatt aaaattttga ttaatattat agcacaatag cacaaaatat aaggagacta
     3361 aaaatgaaga agtttataaa agtttcattt tttattttgg ggatttttac actattcata
     3421 gctataaata aattagtata tagagatatg gtaagtgtca aagattatga aataaatcat
     3481 ttagaggaaa ttgtttttga ttttgatagc aggagaagta gagatgttgg tactattgta
     3541 agatttaaaa taagcaataa tagtaaatat agttataaat taaacagtgc taaaatgaag
     3601 tttgaaaatt gtgtggaaaa taaaaaggga atgaataagt gttctgcata tgtaaatgta
     3661 gatttattta atgaaaaaaa ttctataact gaaagaactc tgaaatatgg aataaaacca
     3721 aataatcaag gttatgttga ttttgtaatt ccaaaaggac taaattttga ttataggtac
     3781 tttaatgaat ctggtatgag tgtagagtat aaaggagaat atattgtcaa tattccaatg
     3841 gcaaaaggac tttatctagt agttggaaaa aataatgata catggaatgc taatatcatg
     3901 gaataatttg aaaaataaat caattataga tataattaag aataatatga aatttataaa
     3961 agttcagact aggagggata tttaatgtca ataagtaatg taagagagta ttttaaacaa
     4021 tttggcaagg aagacagtat attagaattt gaacaatcta gtgctactgt ggaacttgca
     4081 gctgaagcag ctggggttat tccagcaaga atagctaaga cactctcatt taaaatagga
     4141 gatgatgcaa ttttaattgt gacagctggt gatgctaaga ttgataataa gaagtataag
     4201 gctgaattta attgtaaggc taaaatgttg acaccagaag aagtcttgga gtttactgga
     4261 catgctattg gtggagtctg tccatttggt ttaaaaaatt ctataaaagt ctatttagat
     4321 gattcaatga aaagatttga tacagtattt cctgcatgtg gcagtagtaa ctctgcaata
     4381 gaacttacat gtgaagaaat ggagaagttt tctaagtgtg aaaaatgggt agatgtttgt
     4441 aagaattggt aaaaaaatat agtaaactat agtaggaatt agtaaaaaac tacctctgta
     4501 tacaatctat acagaggtag tttttttgtg taaatattta taaatctaag aggaaaatct
     4561 aatttaaact aataatccaa aataaagtta aaaaattctg attttttttc gaaacaataa
     4621 aaaaatgaca aaaaagtgga aaaaatgtaa taaatattgt taaaaaaata attagaaatt
     4681 attgaaaaat tatcctttga gtgatactat tatagatgta agatatttaa aaataaaaaa
     4741 tagtcagata aaaatacgat tatttagctc tattaaaata atttttatac gataaaaatc
     4801 ttctgtacat ggctaaaaaa cagatgtaca gacaatattt aatgtttaat ataccctaat
     4861 ataaatatcg taaacaaatt attttatctg taaccagtgt gaaagaaatc ttattctaac
     4921 ttgaaatttc acaaaattcc aaaaagcgag atttttttta agtatagttg attttaaatt
     4981 aacaatttat gattaaatta atgtcataat tggttagtat aatcacatat aaatatagag
     5041 cgtagtttga ttaaggttat aaatttctcg acgtatattc aaatacctta ttatagatgt
     5101 tgccgagatt ttaattaata aatgaaaata aaatatttat aaaatactga ggaggctatt
     5161 atggatttaa attctaaaaa atatcagatg cttaaagagc tatatgtaag cttcgctgaa
     5221 aatgaagtta aacctttagc aacagaactt gatgaagaag aaagatttcc ttatgaaaca
     5281 gtggaaaaaa tggcaaaagc aggaatgatg ggtataccat atccaaaaga atatggtgga
     5341 gaaggtggag acactgtagg atatataatg gcagttgaag aattgtctag agtttgtggt
     5401 actacaggag ttatattatc agctcataca tctcttggct catggcctat atatcaatat
     5461 ggtaatgaag aacaaaaaca aaaattctta agaccactag caagtggaga aaaattagga
     5521 gcatttggtc ttactgagcc taatgctggt acagatgcgt ctggccaaca aacaactgct
     5581 gttttagacg gggatgaata catacttaat ggctcaaaaa tatttataac aaacgcaata
     5641 gctggtgaca tatatgtagt aatggcaatg actgataaat ctaaggggaa caaaggaata
     5701 tcagcattta tagttgaaaa aggaactcct gggtttagct ttggagttaa agaaaagaaa
     5761 atgggtataa gaggttcagc tacgagtgaa ttaatatttg aggattgcag aatacctaaa
     5821 gaaaatttac ttggaaaaga aggtcaagga tttaagatag caatgtctac tcttgatggt
     5881 ggtagaattg gtatagctgc acaagcttta ggtttagcac aaggtgctct tgatgaaact
     5941 gttaaatatg taaaagaaag agtacaattt ggtagaccat tatcaaaatt ccaaaataca
     6001 caattccaat tagctgatat ggaagttaag gtacaagcgg ctagacacct tgtatatcaa
     6061 gcagctataa ataaagactt aggaaaacct tatggagtag aagcagcaat ggcaaaatta
     6121 tttgcagctg aaacagctat ggaagttact acaaaagctg tacaacttca tggaggatat
     6181 ggatacactc gtgactatcc agtagaaaga atgatgagag atgctaagat aactgaaata
     6241 tatgaaggaa ctagtgaagt tcaaagaatg gttatttcag gaaaattatt aaaatagata
     6301 aatattaaaa tatacacaat aggtcgatat taaaatagat atggaggaag gatttatgaa
     6361 tatagtcgtt tgtataaaac aagttccaga tacaacagaa gttaaactag atcctaatac
     6421 aggtacttta attagagatg gagtaccaag tataataaac cctgatgata aagcaggttt
     6481 agaagaagct ataaaattaa aagaagaaat gggtgctcat gtaactgtta taacaatggg
     6541 acctcctcaa gcagatatgg ctttaaaaga agctttagca atgggtgcag atagaggtat
     6601 attattaaca gatagagcat ttgcaggtgc tgatacttgg gcaacttcat cagcattagc
     6661 aggagcatta aaaaatatag attttgatat tataatagct ggaagacagg cgatagatgg
     6721 agatactgca caagttggac ctcaaatagc tgaacattta aatcttccat caataacata
     6781 tgctgaagaa ataaaaactg aaggtgaata tgtattagta aaaagacaat ttgaagattg
     6841 ttgccatgac ttaaaagtta aaatgccatg ccttataaca actcttaaag atatgaacac
     6901 accaagatac atgaaagttg gaagaatata tgatgctttc gaaaatgatg tagtagaaac
     6961 atggactgta aaagatatag aagttgaccc ttctaattta ggtcttaaag gttctccaac
     7021 tagtgtattt aaatcattta caaaatcagt taaaccagct ggtacaatat acaatgaaga
     7081 tgcgaaaaca tcagctggaa ttatcataga taaattaaaa gagaagtata tcatataatt
     7141 taagggggga taaagaatgg gtaacgtttt agtagtaata gaacaaagag aaaatgtaat
     7201 tcaaactgtt tctttagaat tactaggaaa ggctacagaa atagcaaaag attatgatac
     7261 aaaagtttct gcattacttt taggtagtaa ggtagaaggt ttaatagata cattagcaca
     7321 ctatggtgca gatgaggtaa tagtagtaga tgatgaagct ttagcagtgt atacaactga
     7381 accatataca aaagcagctt atgaagcaat aaaagcagct gaccctatag ttgtattatt
     7441 tggtgcaact tcaataggta gagatttagc gcctagagtt tctgctagaa tacatacagg
     7501 tcttactgct gactgtacag gtcttgcagt agctgaagat acaaaattat tattaatgac
     7561 aagacctgcc tttggtggaa atataatggc aacaatagtt tgtaaagatt tcagacctca
     7621 aatgtctaca gttagaccag gggttatgaa gaaaaatgaa cctgatgaaa ctaaagaagc
     7681 tgtaattaac cgtttcaagg tagaatttaa tgatgctgat aaattagttc aagttgtaca
     7741 agtaataaaa gaagctaaaa aacaagttaa aatagaagat gctaagatat tagtttctgc
     7801 tggacgtgga atgggtggaa aagaaaactt agacatactt tatgaattag ctgaaattat
     7861 aggtggagaa gtttctggct ctcgtgccac tatagatgca ggttggttag ataaagcaag
     7921 acaagttggt caaactggta aaactgtaag accagacctt tatatagcat gtggtatatc
     7981 tggagcaata caacatatag ctggtatgga agatgctgag tttatagttg ctataaataa
     8041 aaatccagaa gctccaatat ttaaatatgc tgatgttggt atagttggag atgttcataa
     8101 agtgcttcca gaacttatca gtcagttaag tgttgcaaaa gaaaaaggtg aagttttagc
     8161 taactaattt cttggggagg gtattatgag tacaagtgat gttaaagttt atgagaatgt
     8221 agctgttgaa gtagatggaa atatatgtac agtgaaaatg aatagaccta aagcccttaa
     8281 tgcaataaat tcaaagactt tagaagaact ttatgaagta tttgtagata ttaataatga
     8341 tgaaactatt gatgttgtaa tattgacagg ggaaggaaag gcatttgtag ctggagcaga
     8401 tattgcatac atgaaagatt tagatgctgt agctgctaaa gattttagta tcttaggagc
     8461 aaaagctttt ggagaaatag aaaatagtaa aaaagtagtg atagctgctg taaacggatt
     8521 tgctttaggt ggaggatgtg aacttgcaat ggcatgtgat ataagaattg catctgctaa
     8581 agctaaattt ggtcagccag aagtaactct tggaataact ccaggatatg gaggaactca
     8641 aaggcttaca agattggttg gaatggcaaa agcaaaagaa ttaatcttta caggtcaagt
     8701 tataaaagct gatgaagctg aaaaaatagg gctagtaaat agagtcgttg agccagacat
     8761 tttaatagaa gaagttgaga aattagctaa gataatagct aaaaatgctc agcttgcagt
     8821 tagatactct aaagaagcaa tacaacttgg tgctcaaact gatataaata ctggaataga
     8881 tatagaatct aatttatttg gtctttgttt ttcaactaaa gaccaaaaag aaggaatgtc
     8941 agctttcgtt gaaaagagag aagctaactt tataaaaggg taaaaatcta attaaaaata
     9001 tatttgctgt taactagatt aaatagtatt ttaatctagt taacagaata taaatactaa
     9061 aataacagga ggttataact atgaaattag ctgtaatagg tagtggaact atgggaagtg
     9121 gtattgtaca aacttttgca agttgtggac atgatgtatg tttaaagagt agaactcaag
     9181 gtgctataga taaatgttta gctttattag ataaaaattt aactaagtta gttactaagg
     9241 gaaaaatgga tgaagctaca aaagcagaaa tattaagtca tgttagttca actactaact
     9301 atgaagattt aaaagatatg gatttaataa tagaagcatc tgtagaagac atgaatataa
     9361 agaaagatgt tttcaagtta ctagatgaat tatgtaaaga agatactatc ttggcaacaa
     9421 atacttcatc attatctata acagaaatag cttcttctac taagagacca gataaagtta
     9481 taggaatgca tttctttaat ccagttccta tgatgaaatt agttgaagtt ataagtggtc
     9541 agttaacatc aaaagttact tttgatacag tatttgaatt atctaagagt atcaataaag
     9601 taccagtaga tgtatctgaa tctcctggat ttgtagtaaa tagaatactt atacctatga
     9661 taaatgaagc tgttggtata tatgcagatg gtgttgcaag taaagaagaa atagatgaag
     9721 ctatgaaatt aggagcaaac catccaatgg gaccactagc attaggtgat ttaattggat
     9781 tagatgttgt tttagctata atgaacgttt tatatactga gtttggagat actaaatata
     9841 gacctcatcc acttttagct aaaatggtta gagctaatca attaggaaga aaaactaaga
     9901 taggattcta tgattataat aaataagaat ttgggaatta aaagtttaaa taaaattgtt
     9961 taaaaaacaa tttcggatat atgaaaaatc taatttaatg ggggtaatga atatgagaga
    10021 agtagtaatt gccagtgcag ctagaacagc agtaggaagt tttggaggag catttaaatc
    10081 agtttcagcg gtagagttag gggtaacagc agctaaagaa gctataaaaa gagctaacat
    10141 aactccagat atgatagatg aatctctttt agggggagta cttacagcag gtcttggaca
    10201 aaatatagca agacaaatag cattaggagc aggaatacca gtagaaaaac cagctatgac
    10261 tataaatata gtttgtggtt ctggattaag atctgtttca atggcatctc aacttatagc
    10321 attaggtgat gctgatataa tgttagttgg tggagctgaa aacatgagta tgtctcctta
    10381 tttagtacca agtgcgagat atggtgcaag aatgggtgat gctgcttttg ttgattcaat
    10441 gataaaagat ggattatcag acatatttaa taactatcac atgggtatta ctgctgaaaa
    10501 catagcagag caatggaata taactagaga agaacaagat gaattagctc ttgcaagtca
    10561 aaataaagct gaaaaagctc aagctgaagg aaaatttgat gaagaaatag ttcctgttgt
    10621 tataaaagga agaaaaggtg acactgtagt agataaagat gaatatatta agcctggcac
    10681 tacaatggag aaacttgcta agttaagacc tgcatttaaa aaagatggaa cagttactgc
    10741 tggtaatgca tcaggaataa atgatggtgc tgctatgcta gtagtaatgg ctaaagaaaa
    10801 agctgaagaa ctaggaatag agcctcttgc aactatagtt tcttatggaa cagctggtgt
    10861 tgaccctaaa ataatgggat atggaccagt tccagcaact aaaaaagctt tagaagctgc
    10921 taatatgact attgaagata tagatttagt tgaagctaat gaggcatttg ctgcccaatc
    10981 tgtagctgta ataagagact taaatataga tatgaacaaa gttaatgtta atggtggagc
    11041 aatagctata ggacatccaa taggatgctc aggagcaaga atacttacta cacttttata
    11101 tgaaatgaag agaagagatg ctaaaactgg tcttgctaca ctttgtatag gtggaggaat
    11161 gggaactact ttaatagtta agagatagtt ttagattata atatttacaa attaaatatt
    11221 taattatgaa tttaacctgt aagaataact atttgttatt cttacaggtt tttatagtta
    11281 aaaataatta ttatctatta aacaaaaata gtgtaaaata tataatatca tgattttgaa
    11341 gatataaaaa agtaggaaga tataaaagaa agtaggtaaa agtttgttta aaaaagagaa
    11401 tcaaagatat aatcttatat cgtatacaaa tgaagaagaa atgactttaa aagaagtttt
    11461 attggacaag ctaaatttct cagttaggtc attatcaaaa atgaagaggg aaaagagtgt
    11521 tttagtaaat ggagtatata aaaaaccgag tttaaaggtg tatagtggtg atttaataga
    11581 agtaaaaata gacgaggaaa aagcaaattt tgaacctcaa gatttaaatt tacagataat
    11641 ttatgatgat tttgatataa ttatggtgaa taaacctccg tttatggtag ttcacccaac
    11701 aaaaagccac tatgataaaa caatagcaaa tggtattagt tactatatag ataatcaaaa
    11761 agaaaatgta aaaataagat ttgtaaatag gttagatatg aatacttcag ggttagttat
    11821 agtagcaaaa aatgcttatg cacatcatac tttgtctaca gcaatgagtg aaaataaagt
    11881 tgaaaaaaag tatataacag tagttgatgg aattataaaa gaaaatgagg gaactataga
    11941 tgagccaatt tatagaccta cagaagattc aataaagagg ataatagatg aaagaggaca
    12001 gtcatctgtt actcactata aagtaataga aagactagaa aatgcaactg tattagaagt
    12061 aagcttagaa actggtagaa cacatcaaat aagagtacat atggctcata taggtcatgg
    12121 aataataggt gatgagttgt atggttatgt agatgaagat ctaataaata ggcaagcact
    12181 tcatgcatat aagctagagt ttgaacaacc aagaacaaaa gaaaaattga aatttaaagc
    12241 agatatacca gaggatatga aagagttaat atcgaaatta agataatggg gtgatatata
    12301 tgataataca taaatttata atacatgttt tggacaaaaa tagtgatgtt ccaatattaa
    12361 atgactttga aggcaaagtt aatcaagaag tggatggttt tttccagaaa gctataaaaa
    12421 gaattgcaaa agatgaggat ttaagaaaag gtgtttttaa agattataat gacaatttaa
    12481 ttaaaaactg ttgtgaacaa ataatatatg atgaaagtac ttttttgaaa aattctaaag
    12541 aaattgcatc ttacttgttt gacgttatga aaattaatgc tgcactagaa tcttgtgatt
    12601 tggcaatttg tttatacact attaaagatg aaaagagtgt agcaatactg aaattagatt
    12661 ataaaaagtt gtacactcat tcaattgaat ttgtggatga taaatttaat attcaaatgg
    12721 tttcaaatga aattggtatc ccagagactt taagacaaaa gcaaggagct ttgattagtc
    12781 taagtggtat aaatgatgag tttcatctaa ggcttttaga taaggatgct gaaaaagaag
    12841 ggtcagaatc taagtttgta acagaatttt taaatgctaa aaaaatagat gatgataagt
    12901 acaagaccaa ggtatttaaa aatacagctg aaaattggat aaccaatgct cttagtaatg
    12961 atataaagca agctgaagat gtaagaagta tattaaatta tacactgaag gaaaaacatg
    13021 aagttgatat aaatgacttt gttgataatt caattaaaga tgatgagtta aaggatagct
    13081 ttaaagagca tatggaagaa aaaggtcttg atgaaagttt tagtatagat aaaaaatggg
    13141 ttgagaaaaa gcttaaaaaa agaagtataa aaacggataa tggttttgac ataaaaggga
    13201 atctaactga ttttgaagac ccaatgaagt atactgtaaa acaaaatcaa gatggaacta
    13261 tagatataat aataaagaat gttacgtttt atgaagaaaa gtaatttata tacttagtat
    13321 ataaattaaa tataagagcg tgtattttat atattgtaaa taaatgtaat ctagtctgtg
    13381 cgaactctaa tataatttat tatgtagaag tttacacaga ctatttatat ttgtagacaa
    13441 aacgatacta tttgttttct tcgatatatt tacatatatc accaatacat ttaaagttct
    13501 cagcttcctc gtctggtatt tctattccaa attcatcttc aagggccatt ataatttcaa
    13561 cagcatctaa tgaatcagct tctaagtcgt ccattaaaga tgcttccata gttatctcat
    13621 ctaaattgtc tacccctaat tgttctgcta ttatttcttt tattttctca aacattttaa
    13681 gcctcctaaa gcagttttaa ttaattttaa tgttagttta atataatatc aagaattttt
    13741 caatatggaa tctataaaaa ataataaaat tttataaatg tagtaataaa gtattatttg
    13801 atgaataaac tttattgata acaaatataa cttaggagga aatgctatga aatatgtagg
    13861 actattacta acttcagtag gaatgttttt acttatagca gtaaattttt attataattc
    13921 aataacttta gatatgcaac gtatagagga ttatgtaatg gagaccaatt taatccttga
    13981 agatgtagct gaaaaggaga gttatgtatc taatgaaaaa gaagattaca tatcaaggtt
    14041 aatgcatgta aagaaaggaa tagaaaattc aaaaacatcc ttcttgatag aaagatataa
    14101 ggagtacaaa ataaaatcaa tagaaagttt aatctatact atatcagagg agaaaaaaga
    14161 ttatctggat gaagttgata gatacaataa acttggtgaa aaagaaataa ataaactaat
    14221 caataaaaac tttttagaag taacttatct gtctattact acatatattt aattaaagag
    14281 acaaaactta gtctcttaat gattcaagga ggattagtta tgacaaaatt agaagacaat
    14341 atggaacgtt tctttggagg atgcgaagga ttttttggga ataaaatgtt gataataata
    14401 gttatagtat tcttactatt atgcacagac attttagaag acttattatg cgatgacaat
    14461 atatggatat gggttatatt aatagtattg ttattattta acttcgatga tggttgttgt
    14521 tgctaaaaaa tattgattat taaataggta aaaccagtaa cttatacaag ttatggtttt
    14581 acctttttta gttcaaatta aaaaatttaa aaaaatagta aaaatacatt agcaaaatgg
    14641 aaaccaaatg catagtatat gttaggagcg aaaagctcaa caaaatatca acaggaggta
    14701 acgtataatg ttagatagat tctttggaga agaaggatgt tttggtggag gaggatggtg
    14761 gataatagta ctattcttcc tattcttagc ttttggagat agctgggcag aatttgacat
    14821 aatggcatgg ataccattcc taatagtttt attaatactt tgctgctgtg gtggatgctt
    14881 agaataagaa cacagttatt aaaaaaagga gggctttgtt atgttagata gattttttgg
    14941 agaagaagga tgctttggtg gaggaggatg gtggataata gtactattct tcctattctt
    15001 agcttttgaa gattgctgga gagacattga cataatgtca tggatacctt tcttaatatt
    15061 attgctaata acttgttcat gtggtggatt ctttgatggt gaagtaggtt gcggttgcta
    15121 atacttaaat cattagaata aaaaaatgct ggtattttat accagcattt ttttatttta
    15181 ttttttaata tgatttaaat taagaatgaa tattattaaa tttgttgaat atagggtata
    15241 atataaaata taatgtattt aaatgtactt gcttttatgg taaaataaaa acttatataa
    15301 atgtagttaa aataggaacc ttcaaaaatt tggttcagtt ttagataaat ttagtaaaat
    15361 aaggaggatg aagatgaaag gcaatataac gataaaagat gttgctaaac aagcaggagt
    15421 gtcaatatct actgtatcta gagttataaa tgattcaaaa cctgtaactg atgaagtcaa
    15481 acaaaaagtt ttagaggtta taaaagagac tggatatata ccaaatccac ttgctagaag
    15541 cttagtaaca aagaagagtc aattaatagg ggtaatagtt ccagaagttt cagattcttt
    15601 tgttaatgag gtgttaaatg ggatagaaga ggttgctaaa atgtatgact atgatattct
    15661 tttagcgaat acatactctg ataaggaaca agaacttaag agtataaatc tattgagagc
    15721 aaaacaagtg gaaggtatag ttatgatttc atggatagtt gaacaagaac atatcaacta
    15781 tatacaaaat tgtggaatac cagcgacata tataagtaaa actgctagaa attatgatat
    15841 atatacagta agtactagca acgaagaagc tacttttgat atgacagagc atcttataaa
    15901 gaaaggtcat gaaaagatag cttttataat gacgagtaaa gatgatactg ttttagaaat
    15961 ggaaagactt gctggttatg agaaagcact ttcaaataac aatatagaat tagacaagag
    16021 tttgattaag tatggtggaa ctgattatga gagtggatac aatagtatga aagaactatt
    16081 agatgatgga ataatacctc atgcggcttt tgtaacaggt gatgaggctg ccataggtgc
    16141 tataaatgct atatgtgatg ctggatataa ggttccagaa gacatatcag ttgcaggatt
    16201 taatgatgtt aagatagcta gaatgtatag acctaaactt actacagtat atcaacctct
    16261 atacgatatg ggagcagtag caataagaat ggttataaaa ttaataaata aggaattaat
    16321 tgaaaataag aaaatagaat taccttatag aattgttgat agagaaagtg tcacagaaag
    16381 aaaaaaataa aactattaaa atcaatcagg tagtgtctaa ggcactacct gattttttat
    16441 gtcaataata tcaaatttaa taatagatta actaaaaata attatctaat tacttaaata
    16501 gtttattttt atctattcat gagggttaga ctttttagtt ttcttaaata aacttttaga
    16561 ttttttttgt tgactacctg gtgtattttt tggtgcaggt gaatttgact tacctttagt
    16621 aggtttagcc ttcttagttt tagctggttt agtatctctt tggttttctg catctgagat
    16681 tttttccatt atattatcca gttttttgcg ttgattttca tctaaaaatg ctttgaattt
    16741 tgatactact tcatctctgc catctaggtt cttacctatt tttataagtt cctccataag
    16801 ttcattttct gattttcctt tgtaactatc agccatcttt tctattttat ccttgtcaat
    16861 atttttttgt ttagccattt tttctaatga ctctgtatct aattttttca taaaaaaacc
    16921 cctttctata aatatatatt gaattatatt catactattt atatatagaa ataatattga
    16981 agtggtgaat tgtgagtgat atataatata aataaaaatt cttatagata atatatatgc
    17041 actgagcttt aaaaatggaa cattaaaata tgaaaaacat actttaatat agggcaatta
    17101 tataatataa gggggaattg tcttgaataa cttactattt ttgattggag tgattgcatt
    17161 gagtaatggt aatatctcta tatttgatga taaaaataaa tcaaaaggaa ataatagagt
    17221 tagaaaaaac aaaaaaaatg caaatgataa actttcacag tctacacatg gcaatttaag
    17281 gtttgatgca aatgatataa aaagaggttt aaggctcatg gatttaagtg aagaagattt
    17341 agaaatggga atggaaataa taacaagaac taaaaaatac atgtcaagag atgaaagaaa
    17401 aatactaata aaaatagaaa gtgttttaga tttagttaga ggaataaaaa aattaaataa
    17461 tatagatatg gtagatacag aagatgaaac ggattttttc agaaaaatgg atagtgatga
    17521 taaaaagaat atgatgataa aggaaattat agatgtattc ccagaaaaaa gaaaaaattc
    17581 agttgaaaaa gctataggaa tgaaaaagaa gatagattta tttgcagaat tatttttacc
    17641 agatgacttt ggtgaaggtg gatttagttt gagctctttg gcaaatataa acaatcttgg
    17701 tagtatgaat aatctcaaat tgttaggaag tcttttaaga ggtgatgata gtaataatga
    17761 tgatgaagac aataatgatg agttaaatta tgaagacgaa gaattagatt gtgaagacaa
    17821 agaattggat gatgatgagt taacagatga aggtgaaacg gaggataatg aagatgaatt
    17881 agtagaagat aagaacaaag gctatgaaaa tatagaaaat gatgaagata tagtatacga
    17941 tgaggaatta tacaacatta atgagcaaga atccgaatac aattataatg aaaaaaatac
    18001 taaaactaga gctaagaaaa aacgataatt aactaaatac ataaagaaaa aaggaagaaa
    18061 aagtaatgat agaataagat tatctcaatt actatataaa accactttaa aaaattcaaa
    18121 atttattata taataataat atgaaatcct gaaacatacg ataaggcttt tttaaattca
    18181 acctacaaat gaaatacggg agaccgtgtc tggtaaatta taacatgcct agttagtttg
    18241 gacgttaaag gttactggca agtcacccac ctgggagtgc ctagggtgtg aatttactta
    18301 gccaccggtg tttcgggaca aaaaaagtgc tactatttca aatagtagta ctttttttat
    18361 taatatgcat ttttctacta aatacgccta attattgata aattcaaaaa gcaagtaaca
    18421 attaagatta taaaagcata gtattaatta ggcttcaaaa agccaaagac ttaataagaa
    18481 tcgcttacag ggagggcaat gaaaaatgca agattataaa aaaaataaaa gaagaatgat
    18541 gaatcagcca atgtctacaa tgaatgaaga agaagtgtat acagatgaaa taaattcaga
    18601 agacatgaga ggttttaaaa aatcacacca tcataatgga tgtaatactg ataataagtg
    18661 tgagtgccat gatgattgca atccatgcaa cccatgtaat ccatgtaaac ctaacccatg
    18721 caatccatgc aaacctaatc catgtgatga caattgtgga tgccatgaca attgtaaatg
    18781 tgattgtgaa ccatgtgaaa tggattcaga tgaatgtttt gaaaacaaat gtggaccaga
    18841 atgctgtaat cctatatctc caagaaactt ctctgtatca aatgcagtgc catttgcaat
    18901 agaggctaat agaatatttg atactatgca attccaaaca tttacagatg caacaggacc
    18961 aaatggagag ccattaactt ttgaaacaga agtagtagaa gtatttggtt cagttccaag
    19021 tgcaggtcaa gcaagtgtaa ctatagaaaa aatatgctta agtaatgatg gaatagttat
    19081 agacacagga atgacaactt tagaagattt cgatttagac ccattaggag atatagtagg
    19141 aagaaactgt gaaacaactt ttgaatttgc agtttgtgga gaaagaaacg ctgagtgctg
    19201 tagacaagga aaaggcaaat cagtagctta taaacaaaga ggattaactg tagcagttcg
    19261 taatttagta ctagagctaa gaggtagatg tggatgtaca gagttcgttg cattagcttt
    19321 cccagcagtt agagcaggag gtggatgtaa gagaagagtt gattatgtag aatttacttt
    19381 taacacactt tcagcaccaa tatgcttgcc agctgacgga agagctgtta ctttaagaca
    19441 agaatatcaa actaacttaa ctgtagattg tataggaaaa tctatattaa aattagaatg
    19501 caacgaatgt tgtgaacctt tctatgaatt aattatacca aatgatatag atttagtact
    19561 ttgcttacaa gaaacagtta gcacattaat aagtgaacaa atagtagttt tagcatcacc
    19621 aaatccaatc caaccaagac ttgttgatac tttctctaaa gtatgtgatt tttcgcaatg
    19681 tggacctaat catggaagtg gaaagccaag ttgccacaga tagataggta gatatataga
    19741 cagtttaaat tataaataaa aatcatctaa ggaaggctac caaagtgtat aatttacatt
    19801 ggtagccttc acgtttttat aactagcgta tagattttag tgatttaaaa agcactatag
    19861 tatgtaatag acaagtaaag attgatacta tcgtcattgt gtatatgtat gcataaatat
    19921 taagtcctgg aattggaaga aatacatata aagctattag ttgaattaac atgccagtaa
    19981 tagtatttat acttgaagct aattcttttc ttattgaatg taaaatgcca gatagagttt
    20041 gatttaggga cataaatagt ggaactaaaa acatagcttt tagatattct cctgcaagtg
    20101 tatttttaaa tactaatata cataaatctt ttccaaagaa ataaaaaaat attgatgata
    20161 gtatgcctac aaataaagta agaagtatgg agtatttgat ttttttaatt acactcttac
    20221 gctttcttag agccatttct tgtgaaatag ttggtattaa atttacaata agcgctgaac
    20281 caacagtaaa aggtaaaaat ataaaaggca ttaccatccc actgacaata ccatacatac
    20341 ttaaagattg ttgataagtc attcctgata atgctaatct tgatggtatc atcatagaac
    20401 ttatagattg caaaagtatg tttgacattc tattacaagt tatgggaata gacattttta
    20461 atgtttccat agaagaatta taaaaatctt ttatgcttat aatataccta ttgtcaaatg
    20521 atgaatctcg ccataagcaa ataagcataa atataatatt actcaactca ccaatcgata
    20581 tacctaatag agctatataa caattcatag atttattatt tatgtacata acaagtaaaa
    20641 aaacaaataa tattttacca atttgttcaa tgatttgtcc aatagcaggg atttttacat
    20701 tctttatgcc atagtaatat cctcttagta cgtttgaaag agttattata actatagcag
    20761 gacaaacagc tagtataaat agatttaatt tagcatcttt taaaaattta agtgataaaa
    20821 aagaactatt gaaagatgca actatagata taattagtgc tacgaaaaat gatatatata
    20881 aagttgatat aaataaagca ttagtattgt gtctatcgtt taaagctttt ctctttgcaa
    20941 ctagacaact aagtgcagta ggtatacctg ttgttgtaac agctaagcaa atcatcaaaa
    21001 agttaaaaat catatgatag atacctaaac ctgtttcacc tagtgctctt gataagaata
    21061 ttttatataa aaaaccaaat attcttacaa taaaattgga tataaataaa ataactgttg
    21121 atagtactaa ataaggtact ttcaaaaaaa tcacctcctt atcttttaaa tatattcaca
    21181 aggaggtgta aaaatttata attttataaa ttctggtttc aatttattaa atttagatac
    21241 atatttaaat cccatatttt ttagtaatga atatatgtag tcaaactcac atgcaacttg
    21301 acttatatga tgtgaatctg aacctgtggt tatgatttcc cctcccaagt ctttgtacat
    21361 ttgaagtata tacttagatg gggttaggtc tggaagatta tatctataac aagaggtatt
    21421 tatctcgata cctttgccat cataaatagc ttgtttaaga gtttcttcaa ttatatcaga
    21481 aaatagtcta tcatctaaaa tagtgtcata aggtgcatac cttttaatca aatctaaatg
    21541 acctaagaca gagtaatctt tataattctt aacaatatta tataaataaa gatagtaatt
    21601 ttcataaact tcatgttgag ttttatcttt aaaataatta ccaaggtata agtccattgt
    21661 atctatagca tggatagaac atattataaa gtccaatgga tattgatgtg cttccttttt
    21721 gtatgtatcc attaattgag tttgaactcc aaattcaatt ccttttttta ttgatatttt
    21781 atctttatat ttgctttgaa gagtctctaa actcttaaaa taatcttcgt agacaattga
    21841 aaaagaatca tcagcatata catcatagtc aacatggtct gtgaaacaaa tttcctccaa
    21901 accgagttca atagattttt ttaccatttc ttccattgta gattttccgt ctgtagagaa
    21961 acttgagtgc atgtgatagt cgtaaaacat aaatatttcc cctttttata aaaattttgg
    22021 tattatatat acatactaaa taatatcaaa tatacataaa agcatcaata aaatataaat
    22081 acaatgagcg gtgataaatt tggaaaaata cagaatagat ttaaatgaga aaaaatcaac
    22141 taaatatata cagatattta atcatataaa agaattgata ataaataaag agttagtaga
    22201 acatgaaaaa ttgcctccaa taagaaaact atcaaatttt ttacaagtaa ataacactac
    22261 tatagttaag gtatacgagc ttttagaaaa tgaaggatat gtatataaaa ttatagggag
    22321 tggtactttt gtatcaagtt taaattctta taaaagcggt atagataaca ggaaaaataa
    22381 gattattgtg caaagagaag atttaattca ttttgacaat gggaatccat ctaatgatat
    22441 gtttcctatc gattcattta aaaatgctgt aaatatggca ttatctaaag atggtagttc
    22501 aatatttgaa tatgatgaag gtcttggctc tgaagaatta agagaaaaaa tggtagagta
    22561 tctaagtgat ggaaatataa aaactactaa ggaaaatatt caaataatat caggtgctca
    22621 acaaggtata gatatagttt gtaaaggact tataagttat tcagatgttg tttttatgga
    22681 agaaccttct tacaatggag caattgaagt gtttaaaagt agaggtgcaa aaataatatc
    22741 tatacctatg ttagatgatg gaattgatat aggtatttta aaattaaagt tggaaaaaat
    22801 aaaaccaaga cttatataca ttatgcctaa ttttcaaaat ccaactggaa tatcttactc
    22861 aacatataaa aaaaagaaat tgatagaatt ggcagaagaa catgattttt atataataga
    22921 agatgatttt ataagtgatt ttgtttttga atcaaaggac aatagaacaa ttagaagtta
    22981 tgatgataaa aatagagtag tatatataaa aagcttttca aaaatactta tgccaggtct
    23041 taggatagga attgtagaaa tgccaaatga gttgttaaaa aaagttttat gggctaaata
    23101 ttcatcagat atatcaacac caggtttaat tcaaaaatca atgtattact atatgaataa
    23161 ttttgattgg aagaattatt tgagtcatat agaaaagata tatactgata agtataaatt
    23221 ggcaaaaaac ttgattaatg acaaattgag tggtaaatta aaagttagaa aatcttgtgg
    23281 aggaataaac ttttttttag aattaccaag aggatataca tctcaagcat ttacaaattt
    23341 tatgttaaac aaaggagtgt caatgcttcc agggacttat ttttttgaca atctagtgga
    23401 tgatagattc tttaggataa atatagctcg cccaagcatg gaagaactag aaaagggaat
    23461 atctataata agtgataata tagatgagtt tttttgtgaa tataaaaaca agttagatat
    23521 aaaaagcaat aaattatttt attaaataaa tagaggtttt ttaggaggaa aaaatgtttg
    23581 acaagaagaa attagatagg attaatgaat tagcaaagaa aaataaagag ggaatccttt
    23641 ctgctgatga aatcaaagaa agagaaattt taaggaaaga atatttagaa aactttagag
    23701 cccactttag atcaagacta gatagtgtca aagtagtttc accagaagaa tatgagcaat
    23761 acatgaaaaa caataaaaat tagaacttag taggaggaaa atatgaactt aaagtacgaa
    23821 tttaagaatg cttgggattt tgaaagaaaa gaaaatacta ttgaacaaat tatgcaatat
    23881 tctagcaatt atatggagtt tttaagtaag tcaaagactg aaagactttc agtaaaagaa
    23941 attataaaat tggctaaaga aaataactat atttcaatag atgaagctat ggaaaaagga
    24001 agcataagtt gtggtgataa gatttatgtt ataaataaag agaaggcagt agcacttttt
    24061 gtaataggta aaaattatat agaaaaaggt atgaagatta taggaagcca tatagattcg
    24121 ccaagattag atttaaaacc aaatccactg tatcaagaga gtaatctagg tttttttaag
    24181 actcattatt atggtggaat aaaaaaatac caatggacgg ctattccact tgctttacat
    24241 ggtatagtta tattaaatga tggaacaaaa gtggacatat ctattgggga agaagatagt
    24301 gacccagtat tttgtgtaac tgatttattg attcatttgg ctggagacca aatgcaaaag
    24361 aaactatcag aaggaattag tggagaagct ttaaatatat taattggaaa tatgccttta
    24421 gaagatgaag aaaaagaacc tataacagca aatatattaa agattttaaa tgagaaatac
    24481 aatatagttg aagaagattt attaagtgct gaaattgaag tagttccagc tggtaaggct
    24541 agagatttag ggcttgatag gtctatggtg ttgggatatg ggcatgatga tagggtatgt
    24601 tcatatgctg ctgtaaaagc tatactagaa actgaacaac cagaatttac ttcagttgca
    24661 ttatgtgtag ataaagaaga aattggttct aaagggaata caggtatgca ctcaaagttt
    24721 tttgaaaata ctgtggctga actgattgct ttagaaggag attattgtga tattaaagta
    24781 agaagagctt tagcaaatag taaggtatta tcagctgatg tttctgctgg atatgaccca
    24841 aattttggag aagcatatga aaagagaaat tcggcatata tgggaaatgg agtagtttta
    24901 actaagtata caggttcaag aggaaagagt ggatgtaatg atgctaatgc tgaatttatg
    24961 tcagaagtaa agagaatttt taataaagga aatgtagtat ggcaaactgc tgaacttgga
    25021 aaagtagacc aaggaggagg cggaactata gcccatatat tggcaaatca aggtgcagag
    25081 gttatagact gtggtgttgg agttttaaat atgcatgcac cacatgagat tgtctctaaa
    25141 gtagacatat atgaaatgta taagggatat aaggcatttt ttaatataaa tttataagta
    25201 aaaatataaa tgtaataagt gaataaaggc tgttgtataa acaatttaat ctgttgtaca
    25261 acagtcttat ttttatgtaa aaatagcaat ttgtcacact agagaaaata aaagaaaata
    25321 ctgtcaaatt aaaaattatt taaaaaaaaa ttgacaacga tttcatcttg ttataatatg
    25381 taataaagaa cttgttatat aatgttataa aaatgttaca tagggaggtg agcaaaatgg
    25441 atgaaaagag acaaaaaaaa gaaatggatt tagaagtaaa ttcctttgtt cctttatatc
    25501 aacaactata tgacaacata aaaaaacaaa tagcatctgg catatataaa ccaggagata
    25561 aacttccatc tgaaggagac ttatgtaaag aatttaatat aagtcgtata actgtgagaa
    25621 atgcacttaa tgaacttgta aaagaagata tattatgtaa aaaacgtggt aaagggactt
    25681 atgtaacaat accagagaga atagaagcaa catgtgcagg aaatagtttt actaattctt
    25741 gtcatcgtat taatgcaaaa ccaagtacca aaataatctc tgttttaata aaaaaagcag
    25801 ataagcaagt agctgaagct ttaagtattg aactagaaga gaaggtaata tgtattaaaa
    25861 gacttagatt aatagatgaa gtacctgtta tttttgaagt tgactatttt agaatagatt
    25921 acatgttttt attaaaagaa gaattagaag ataaatcttt aatggaggtt atatctaata
    25981 atataagcac attacctaaa cgagtagaaa atatatttga agtaaaacat tcaaacaaag
    26041 aatattctga ccatcttaaa tgtgcaagta atatgccact attgaaagta agacaatcgg
    26101 tgtatacaga aaataatgat gtcttatatt acaatgagca atttataaga agtgataagt
    26161 acaaatatgc agtatcagca gaaatataat ctataattta gtaggaggat gagtatgaaa
    26221 aagataatat tagcatctca tggagacttt tcaaaagggc ttttaaatgc agtaaaaatg
    26281 atagtaggag atttagcaga ttgtgtcagt tcgtatggtt tgtaccctgg acaaagtgct
    26341 tcagattttg ctttagaatt agaaaaaaca atatcagaag ataaagaatg tgaatatata
    26401 attttatctg acttatatgg agcaagtgta tgtacagcaa tgttaagatt aactaatcta
    26461 cataatgtta ggttattttc tggaatgaat ttgaatatgg tattagaact tcttacaagg
    26521 ttttctgatg aattaacagt tgaagatata gaccaattag tggaagaatc tagaagagga
    26581 atacaaagcg tgacattaca aattaaagaa gaagaagagg tattttagtt tatgaaaaaa
    26641 aagggaatat tatcagtatt taaaagtaaa aaaccaatta tagcaatgat acacttaaaa
    26701 ggagatactc cagaagatat ctttgaaaga gctaaaaaag agattacaat ctttgaagaa
    26761 aatggtgtag atggaataat gttagaaaat tactatggta attattatga tttagaaaga
    26821 atacttgaat atgtatcaaa agctaattta tcaataccat atggagtaaa ctgtttaaat
    26881 gttgatacta tgggatttga actggcaaca aaatataatg ctagttatat acaggttgat
    26941 tctgtagtag gtcatgttaa acctagagat gaagctactt tagaagagtt ttttaaatta
    27001 caacgttcaa aatgcccagc gtatttaatt ggaggcgtac gttttaaata tcagccagta
    27061 ttatcagaaa atgatgttga agaagattta aaaataggaa tgacaagatg tgatgctata
    27121 gcagttacag aaaatgctac aggtcaagaa acatcaatgg aaaaaataga actgtttcgt
    27181 aaaaatttag gagacttccc attggttata gctgctggag ttacattaga aaatgcaaaa
    27241 aaacagctta aattaggaga tatggcaata ataggaagtt actttaaaga taattacaaa
    27301 gattttgggg atgttagtgt agaacacgta aaaacattca tggatgaaat caaaaaaatt
    27361 agggaggaat tataatgatt aaattagtaa gagtagacca cagacttata catggacaag
    27421 tagcatttac atggacaaag tttttaagca cagactgtat attgatagca agtgatgact
    27481 tattaaaaga tgaattgaga atggcaggac ttaaaatggc taaaccatct aatgttaagt
    27541 tagtaatgaa aagcatagca gattctataa aagcacttaa ctcaggtgtt actgataaat
    27601 acaacttatt gatactttgt gaatctgtag aagatgttta tagacttgct aaagaggtaa
    27661 aagctattaa atctataaac cttggtggaa caaaatcaga tgataatcgt gaaaatatat
    27721 ctaaagcagt gcatgtatca aaagatgata taaagatgat taaagagtta gattcagaag
    27781 gcgtaaatgt atttgtacaa ttagttcctg atgatgatgc tacaaatgta atgaaattaa
    27841 tataatttta gggggatgaa aatggaattt acacaagtta ttttgataac tctaattgca
    27901 ttctttgcat atatgcatag ttttgtaggt tctacaatgc ataatagacc tatagttgtt
    27961 gcaccacttg ttggtttagc actaggtaat cttcatacag gtatagtaat aggttcaaca
    28021 ttagaattag tatttatggg tgcatttcct gttggggcaa gtaatcctcc tgattttgtg
    28081 tctggttcta ttattgccac agcgtttgta atattaacgg gtcaagatgt atcagcagca
    28141 gttgtacttg cagttcctat agcaactcta gtattgctta tagataactt cttaatgaca
    28201 gtagtactta catggggagc tcatattgct gaccgttatg cagaagaggg gaatattgaa
    28261 ggcgtagaac gtgtgcaatt attatttggt ataggaaata aattaatatt agcaataata
    28321 gtaggtatag gattttcatt aggtgttcca gtaatagaaa aaatactttc atttatacca
    28381 tcatatgtaa ctcatggaat ggatgttgca gcaggcgtga ttcctgcaat aggttttgcc
    28441 atgttagcta gaatgatgct taataagaaa acagtagcat ttttattatt aggatttata
    28501 ttggttgctt acctaaacat aacagtaaca ggagttgcat tgtttggatt agcaatagct
    28561 ttaatatatg ttaactttgt aggagagaag gaggtaatag tagatgacaa cgagttctaa
    28621 gaaattagag acaatttcac cagatagtaa aataacgagg aaagactttt ggaaatgttt
    28681 tagaaggtca ttgacattag attcatcttg gaactacgaa cgtatgcaaa atatagcata
    28741 tgcatacatg atggcaccaa taatccgtag attgtataaa gatgataaag aaaagaaatc
    28801 taaggcttta aaaagacatt tagaatttat gtcagttact cctcatataa gtactctttt
    28861 ggtaggtata tctggagcta tggaagaaga aaatgcaaag aataaagaat ttgatgctaa
    28921 tagtataaat gctgttaaat caagtttaat gggtccagtt tctgggatag gagattcatt
    28981 tttctgggga acattaaagc ttatagcagc tggggttggt atagctttag cttctcaggg
    29041 taacataatg ggaccgattt tattcctttt aataataaat gttcctcatt ttattatacg
    29101 ctatatatgt ctagataaag gatttaagta tggaactcaa ttctttaaag atgtgagtgg
    29161 ttcaagtata gtttcaaaag ttatggaagc agcttcaatg ttagggctta tggtaattgg
    29221 tggtatgact gcatctaatg taatgttaaa acttagtgtt aatgtaggta gtggagaatg
    29281 ggcagagcct attcaaactt acttagacca aataatgcca tgtatgcttc cagctatgat
    29341 attcggtatt atgtattggt tattaggaaa aaaggtaaag acaactacga tattaatttc
    29401 agttatgatt atttgtatag tattagctgc tattggagta gtataaataa aaaaatccca
    29461 agttcatttg aacgagggat tttttgacta aattaaataa aaaagaaata cggtttataa
    29521 taatttataa gcatttatgt gatagttata attagcttat taattagtta taaacctaaa
    29581 attaagtact actttattaa agttattata ttataatttc gaaatactca cataatctgt
    29641 ttagaaattt atcttctaat ggggaaaaag ttttcttttt agagtaaata aagaaaaatt
    29701 ttctagtaaa atcaacatcc tttattttat agcattttat tttacctgca ttgagataat
    29761 caatagctga aatgtaggat ataaaggata ctccaagacc taatctaacc atttccttaa
    29821 tagcctcatt tgattctaca tgagctatta catttaattt acttactgga aagttatttt
    29881 tactaagtgt gtttagtatt aagtttcttg tacctgaccc ttcttttctc ataataaaat
    29941 ttagataagc caattctcct atatcgatac aattattttt attatcaatt ttaaaatctg
    30001 aaggagtgat aagaacaagt tcatcatcta ataagtctag gtactctatt tgaggattat
    30061 tgattttaga accaactaat ccaaagctta ttcgttcatt taaaatttct gatatagcat
    30121 attgagaatc ataatggctt atggaaaatt ttacatctgg ataactcata gaaaaactct
    30181 ttataaaatc aggtaaaata taagtttcag gtatggaact acatgctata tctataatac
    30241 cttctatttt tcctgaatat tcttttatat cataaattgc acgtttgcaa ttattcaata
    30301 tatatatagc atgttcataa agaatttcac cagattttgt gagtgtaata actttgttgc
    30361 ttctatttat aaggacagtt tccaactctc tctctaaatt ctgtatgtgt gcacttacag
    30421 taggttgagt taaaaaaagt tctcttgcag cctttgaaaa actctgatgt tttgcaacag
    30481 caacaaatac ttcaagttgt ttaaaatcca ttatttaatc ctccttaaat tatctaaaac
    30541 tattataaca tatttgataa attaatagtc aaaggtgatt tttaaaatag aaaaccttta
    30601 aatattcatg gagtttttga ttgtttagga ataatttggt ataatgttgt aaggtactta
    30661 caataaaaat atcgtaaatt gaattttcaa aattcaactt taaagtgttt tttattacag
    30721 aaaaattaca attagcttct taatcttatt aaattcaaat aaatattata taattatttt
    30781 ataatataat aaatataatt attgaaaaaa gagataactg taaattgaga tatagataaa
    30841 tactataaaa tttaagaaag gaggattgat ttatgaagtt aaaaaggtca ttagtttgtg
    30901 taactatttt aggtattata cttgttggat gtcataaaga aaatactaaa gagaaaaatc
    30961 aagtagcaaa taaagcaaca cagcaaaaaa ctatgacaaa agttcaaaat gatgttaatg
    31021 aaattatgaa taaagattat aagtacatta taaagaatat gggtatacct tataatacat
    31081 tttattatat aaaacctaaa gttttaaaag aatcaaatac aatgcaagat ataaatacat
    31141 caagttatat gacattagtt tatccaaaat atactggaaa tgatgaacta gatggaagtg
    31201 ctttatatgt tgatattaac gaaaataaag ttgtcaatgt tgaaaccaat tctttctcat
    31261 cacaaggtat atcagtcatt gatgctgaat ctagtatagt gattgaaaaa agtgaccatg
    31321 aaaaatcggc tgtatcacta gaaaatttta gacatattga cctgggagag tatgttggtg
    31381 ttgaagattc cagaataaat gaaattgtag gagatgcgaa ttatgattta acagcatata
    31441 atcatgaagg tagtaaagta gttaagagtt atagattgaa agaagataac aaaatattaa
    31501 aaaaagaggt acttacaata agtattgtgg ataacaaaat aaaatctata aaaactattg
    31561 aaagtgataa aattgtaaaa ataataaaag gaactttatt agaataaaat attctaaagc
    31621 atatgatgct gtttcaagat agaaggaaat ttctagctgg gaacagtttt tttattagat
    31681 ttataaaatt tgaactgcac ttatagataa ctattagtta taattaaata taagataaaa
    31741 gtatttccaa cttttataat ctataatata ttacatagaa atatcttaaa taaaaaatat
    31801 attggggagt atttaaaatg atagaattag aagatgccat aaaaattatt gaaaaaaatg
    31861 ttttaccaat aaacagggtc aagatagtaa atcttataga tgcagtaaat aaggtagttg
    31921 cagaagatat atattcaaca attgatagtc ctccatttga taggtcaccc tatgatggtt
    31981 atgcatataa tgcaaattct gaaaatagaa atttaaaggt tatagggact ttgtatgcag
    32041 gagatgtatt taatagggta ttaaataaaa atgaagcagt gaagataatg actggtggaa
    32101 agataccaca gggtgcaaat tgtgtaataa aaaaagaaga tgtaacagtt ataggtgata
    32161 tgataacttt aaatataaaa ttaaaggaat acgataatta catatttaag ggtgaggata
    32221 taaaaaaggg gcaattgatt attaataaaa atcagaatat aggatatgat ggtattggag
    32281 tattagcaag tcttggaata tccaaagtaa ctgtttatga tgatttaaaa gttggaattt
    32341 tgaatacagg tacagaacta caagatataa atgagacttt agagaatggt aaaatatacg
    32401 atagtaatag atatacctta tattcaagat tattaaaact tggaatacaa gcagatacaa
    32461 tatgcaattg ttctgatgat atattggagt tagaaaaaaa ggtaaagaat atgttacaga
    32521 gtgtagattt tttaattacc acaggtggtg tttctgttgg agataaagat ttaattccag
    32581 atgtattcaa aaaaataggg gcaacagaat tattttggaa gataaacatt aaacctggtg
    32641 gagcttgtta tgtaggtaca ttgggagaaa agctattatt tggtctttct ggtaatccac
    32701 atgcagcaat agtggtattt gataatgtga tagttcctgt tattgagtat ttaacatata
    32761 agaagggaac acatcatata aaagctatat ttaaaggaga atttaataaa ctttcaaata
    32821 aagatagatt tgtaagtgga aaactatata caagtgaagg taaattgtat gtagagctaa
    32881 atgataagaa agatgcaaaa gctagattat ctgcaactct aagaagtaat tgtatgatta
    32941 agattaaaaa gaatgagaca ataaaagaaa atcaattagt ggatgttgtt attagatagt
    33001 tttatgaaag gacacaattt tatgtataat atattgtcag ttgtctcata ttcaggagta
    33061 ggtaaaacta cgttgattga aggcataata aaagaattaa ataaaaaagg gtatcgtgtt
    33121 ggagttatta aacatacctg tcatgatttt gatatggatg aggaaggaaa agatacttat
    33181 aaacatagaa aatctggggc gagaaaagtc tgtataatat cagataaacg agtagcatat
    33241 attgaagaac taaaagagaa gaatccatta tataaaatga ttcaattata tgaagatatg
    33301 gacttgatta tcatagaagg atataaaaat tatagattta aaagattgga agtaactaga
    33361 aaaggtaagt atgaggatat tttatctaat aagtctgatt tgataggtat tgtcagtgat
    33421 atagaatata aattgaatat tgaacagttt aatttaaatg attataaaaa tatatcaaag
    33481 tacatagagt cttgtataaa aaatgaaatt ttaagtataa gcaatgttga aataaaaaac
    33541 atattaaaag aataatttta agatgttctt cttatataaa atgatataat tatttatata
    33601 aataaagata taagaaattt atcaatttta ttttgagtta taaaatatta aagaaattgt
    33661 aggaggacaa tatatgaaaa aaatattagt agcctgtgga gcaggaatcg caacatcaac
    33721 aatagtttgt gacagagtag aaagattggt taaagaaaat aatatacaag cagaagttga
    33781 gcaatgtaaa attgctgaat gttcaacaaa acaagaaggt gctgatttaa tcgtatctac
    33841 tactatacta ccaacaactt atgatatacc tacaataaag gcaacaggat atataacagg
    33901 tataaataca acagcattag ataaaaagat attaaatgca ttgaagtagt tttgtattta
    33961 taaaaaaaat ttagtattaa atatagagtt gtatct
//
LOCUS       DAEORZ010000031        33112 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEORZ010000031 DAEORZ010000000
VERSION     DAEORZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33112
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.031"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..763
                     /locus_tag="KRH39_001349"
     CDS             <1..763
                     /locus_tag="KRH39_001349"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HBG8511266.1"
                     /translation="SITDLYEFTKIPVPEHLNDILDIELSDDTIYMITKVDTSKASIE
                     IVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFEIPQNKIVLGENK
                     ILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINITENNTDLIVTLNE
                     GLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSYQEIYELEEANIK
                     SAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            780..1133
                     /locus_tag="KRH39_001350"
     CDS             780..1133
                     /locus_tag="KRH39_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511267.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPSVIR
                     EQVRIRCKELEIPVE"
     gene            1396..1656
                     /locus_tag="KRH39_001351"
     CDS             1396..1656
                     /locus_tag="KRH39_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511268.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(1866..2321)
                     /locus_tag="KRH39_001352"
     CDS             complement(1866..2321)
                     /locus_tag="KRH39_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511269.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(2549..2968)
                     /locus_tag="KRH39_001353"
     CDS             complement(2549..2968)
                     /locus_tag="KRH39_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8511270.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLRKTEYNKNKDEKIDK"
     gene            3267..3476
                     /locus_tag="KRH39_001354"
     CDS             3267..3476
                     /locus_tag="KRH39_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8511271.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            3508..3717
                     /locus_tag="KRH39_001355"
     CDS             3508..3717
                     /locus_tag="KRH39_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511272.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(3934..4332)
                     /locus_tag="KRH39_001356"
     CDS             complement(3934..4332)
                     /locus_tag="KRH39_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8511273.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(4753..7245)
                     /locus_tag="KRH39_001357"
     CDS             complement(4753..7245)
                     /locus_tag="KRH39_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8511274.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGESKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTHTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKDLQSKLNNSINQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNLGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            complement(7259..7801)
                     /locus_tag="KRH39_001358"
     CDS             complement(7259..7801)
                     /locus_tag="KRH39_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8511275.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            8045..9559
                     /gene="glpK"
                     /locus_tag="KRH39_001359"
     CDS             8045..9559
                     /gene="glpK"
                     /locus_tag="KRH39_001359"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG8511276.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVDDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            complement(9673..10302)
                     /locus_tag="KRH39_001360"
     CDS             complement(9673..10302)
                     /locus_tag="KRH39_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8511277.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            10890..11681
                     /locus_tag="KRH39_001361"
     CDS             10890..11681
                     /locus_tag="KRH39_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBG8511278.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            11710..12930
                     /locus_tag="KRH39_001362"
     CDS             11710..12930
                     /locus_tag="KRH39_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBG8511279.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            12950..13645
                     /gene="pxpB"
                     /locus_tag="KRH39_001363"
     CDS             12950..13645
                     /gene="pxpB"
                     /locus_tag="KRH39_001363"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBG8511280.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            13675..14661
                     /locus_tag="KRH39_001364"
     CDS             13675..14661
                     /locus_tag="KRH39_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBG8511281.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRLGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(14880..15446)
                     /locus_tag="KRH39_001365"
     CDS             complement(14880..15446)
                     /locus_tag="KRH39_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8511282.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLIIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            15798..16865
                     /locus_tag="KRH39_001366"
     CDS             15798..16865
                     /locus_tag="KRH39_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBG8511283.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            16884..18044
                     /locus_tag="KRH39_001367"
     CDS             16884..18044
                     /locus_tag="KRH39_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG8511284.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            18057..20153
                     /locus_tag="KRH39_001368"
     CDS             18057..20153
                     /locus_tag="KRH39_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBG8511285.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLVEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDRY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(20312..20656)
                     /locus_tag="KRH39_001369"
     CDS             complement(20312..20656)
                     /locus_tag="KRH39_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG8511286.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            20948..21838
                     /locus_tag="KRH39_001370"
     CDS             20948..21838
                     /locus_tag="KRH39_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG8511287.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            22056..23342
                     /locus_tag="KRH39_001371"
     CDS             22056..23342
                     /locus_tag="KRH39_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG8511288.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(23431..23874)
                     /locus_tag="KRH39_001372"
     CDS             complement(23431..23874)
                     /locus_tag="KRH39_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBG8511289.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(23898..24692)
                     /locus_tag="KRH39_001373"
     CDS             complement(23898..24692)
                     /locus_tag="KRH39_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBG8511290.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(25258..25734)
                     /locus_tag="KRH39_001374"
     CDS             complement(25258..25734)
                     /locus_tag="KRH39_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG8511291.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDDPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(25835..27004)
                     /locus_tag="KRH39_001375"
     CDS             complement(25835..27004)
                     /locus_tag="KRH39_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8511292.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVILHVKNVLSF"
     gene            27167..28030
                     /locus_tag="KRH39_001376"
     CDS             27167..28030
                     /locus_tag="KRH39_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBG8511293.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            28101..28694
                     /locus_tag="KRH39_001377"
     CDS             28101..28694
                     /locus_tag="KRH39_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBG8511294.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(28945..29580)
                     /locus_tag="KRH39_001378"
     CDS             complement(28945..29580)
                     /locus_tag="KRH39_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG8511295.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(29660..30391)
                     /locus_tag="KRH39_001379"
     CDS             complement(29660..30391)
                     /locus_tag="KRH39_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG8511296.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            30722..32911
                     /locus_tag="KRH39_001380"
     CDS             30722..32911
                     /locus_tag="KRH39_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBG8511297.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT        12559 a         3888 c         5298 g        11367 t
ORIGIN      
        1 atcaattact gatttatatg aatttactaa aataccagtt cctgaacatc taaatgatat
       61 tttagatata gagctttcag atgatacaat atacatgata acaaaagtag atacaagtaa
      121 agcatctata gaaatagttg aaaaatcaat atctcaagtg agagttgtag tacaagaccc
      181 taataatgtt atagaaaaac ttgaaatgtt tataaatgat gaattaatat ctactaagac
      241 taatttggaa ataaatagca ttatatttga gataccacaa aataaaatag tattaggaga
      301 aaataagata ctgattaaag ccagtagtcc tacaggcgat ttatattcaa gtatgtttat
      361 atttaaatca gaaacagggc ttaaagtaaa aaaggattct attttaatga taaacaataa
      421 agtatattca atcataaaca ttactgaaaa taacactgat ttaatagtaa cattaaatga
      481 gggattaaag gatgacatga tggaaaacaa tcctatatat caattaataa ataaaactaa
      541 agttcaagta aaaataaata aatctgactt attcaaagac atgaaactag ttgaaatcaa
      601 aaaatcagac tcaagttacc aagaaatcta tgaattagaa gaagccaaca taaaaagtgc
      661 tcagcctaaa atcatagtag aaaaaggaga taaatggaca gctataaaac gtccatctat
      721 gatttttaga tatgatgctg aaaacaacga gccacaagct taaaatggag gtgtgaaaat
      781 tgtttaaatt cgataaaaat aaaatagaac aaatcaaaca aggtagaaaa gtagaaatgc
      841 agtataaaga tatctcagac ataagtatag gtcaagtaaa gcaagatgat gatataacaa
      901 ataattttat agcaaatgca gaaatatatg agatgttgtt aagtcaaagt tctgtcaatg
      961 aagcaagtaa tataagcact tttagtgtaa gaaaatctgg aggtgagagt ggaatggtag
     1021 aagtatatgt agctttaatt ttaagaggca aaaaaacaat agaagaagta ccatcagtaa
     1081 ttagagagca agttagaatt agatgtaaag aattagaaat accagttgaa tagtaaattt
     1141 agaataacta tgtattagtt atttttttta tgtaaagtac aaggtcttaa ctttaataag
     1201 taggccttgt acttattttt tgttatatta gaaattgtat atatatttat tatttattca
     1261 atctataaat tacacctaca atttaaagta cagaagatta aattgataat cttgaaaata
     1321 taatattgca tgatgtaaga atacaacaaa aattaaagct ataagtataa aaaatttaga
     1381 caataggagg ctataatgga taaattaata accgaattga gtagtctagg ggcaataggt
     1441 atactatgtg ctctattatt taaaaatact atgcaggaga aaaaagaaga tagagacatg
     1501 tataaaaaaa ctgtagaaaa ttttatagaa ttatctacac aacaacaaga aataaacaaa
     1561 aatatacttg ttcaaatggg tataatgaaa acagatgtag aggaaattaa ggaagatgtt
     1621 actgatataa aaggtatgtt acaaaacggt gtataacatg aaagtatcag tagtaccaga
     1681 ttatatattg ttagaaaaag ataaagtagt attgtagata gtaccctatt ctattgagaa
     1741 ggatttaata ttttaaaata ttaattaaaa aagtaataaa aataacatat aaaaattcaa
     1801 aaaggagttg agattaaatt tgattagaaa aaatcaattt taagacaact ccttttttta
     1861 ttaaattatt gtctattaac caaaatagct attttagcat ctggattata acttatctga
     1921 accatttgat ttttcttaac atgttcaagg tcttcaccac cataagctat ttgtaactta
     1981 actggtaact taccttgttt tataatagca acgtactctt ttttaccttt tttctctaaa
     2041 ctaatcaaat tgccaacata aggtttaaag ttctgatact ttttactaga atttcttatg
     2101 tagaagaaag caccaacagc aataactaaa tttatgccaa gtgtaaccca agaattgatt
     2161 ttaagcatag ctccagcgat tattatcacg aacattaaaa cgataggtaa tatagctttc
     2221 ttaagaagca atttacccat tatttcatta gcctttttct cagggccact catagttttt
     2281 gatctagcaa atgattgcgc gaatttgtct cttaagccca ttttatcctc ctaattttaa
     2341 taaatattta gttataataa cgagatatta cttgaaacta aaaatttact acatttatat
     2401 tatgtttgac ttttgtataa ataattacat tcaagtaaag caaaatatac taattatttt
     2461 atcataaaat tataaaaaag aaaataaatg aaataaaaat attagaacaa agaaatgatg
     2521 taaaatcgta tcaaaagcaa cataaaaatt atttatctat tttctcatct ttatttttgt
     2581 tatattcagt ttttcttaaa tctttctctt tttcatattc atgaagtttt aattcaatca
     2641 taccttctat tttggcttta tcataatcat ttaactttct aaagttgttt aaaagcttta
     2701 tttcattaga gtttatgcta ttaagtggat agtttgagga ggaatcgcaa attaaatctg
     2761 atttatgtga tagattatct ccatttaata gccagtctac tgaaacatta aatatctcag
     2821 ctatagattt taatatttca taatttggtt ttctaatgtt tctctcaaat ttacttaagt
     2881 tgtcacagcc caacatttct tctagttcat attgtttaag gttttttgct tttctcaaat
     2941 aaacaattct ttctcctaaa gtatccataa acactctcca ttcaattaat gtcaaaaaga
     3001 ctttttaaga tgtaaatagt ttcaaattaa aggtcaaaat gacataaaaa ccattgactc
     3061 aaggtcaaaa tgactttata attaacttaa tgatacgaat ttacatccta attttagcac
     3121 aaagtaatca aaaaatctta tttagtatta aataaattta tatacttaat atgtgtacat
     3181 attaaaaata tatactaaat agagggggtg cgtaaaccaa aataatataa aagtaaatat
     3241 aaatcactta gaaaggaagt tgataaatgg atgctcgaaa aaaatggata ccttttttgg
     3301 gagtgcaagt caagcaaaga cttattgaat taaatatgac tcaaagggaa ttagcgaaga
     3361 aaataggtgt taatgaaaac tatttgtcag ctattttaaa tggaagaaga acaggtaaaa
     3421 aatataaatc atcaatttat caattactta atatagaata ttcagaagat gattaataaa
     3481 tagtatataa agtaggtgaa tattcttgtg tgcaaattgg attcagatgg ggttatagag
     3541 tgttgtagag caattgatga ttttattaca gcactcagta atataaaaag cttaaatatg
     3601 gaaagactaa atactttaac taaatattct agtacatgtt caatccttct taaagagggg
     3661 aattatgaag gatgtacaat tgtgtataga aagatgttgg aagaattaaa aacatgagta
     3721 atgcatttct taggaacata aattatacat ggaaatgtat tatatttttc aaagtacgta
     3781 aactaaaata tggataagat aatctaaata ttataaatgt gcttgaaatt agactatact
     3841 tgtttttaaa taatccaata tccatatttt agtaatatac tacaaaaaaa gaaagttaat
     3901 agatgatgta aaatcgtatc aaattatgta tgtttaaacc attttatctt cattattatt
     3961 agaggaatgc ttttttaagt ctttatattc agatatctta agttcaagta ttccttctat
     4021 ttttatttta tcacgttcgt ttagttgtct gtatagattt aatatcgtca tttcatcatt
     4081 agtaacatgt aggtaatctt ctttatcttc ttttacacta ctattgacat ttaccttctc
     4141 tttaccatag agaagccagt cagtcgtaac attgaaataa tcagctattg acattagtat
     4201 atcacaatta ggttttctat ctcctgtttc atacttgcct aagttttcaa attttaaaat
     4261 atccataagt ttgcgctgag taagtttttt ggagtttctc aaataagcaa ttctttttcc
     4321 taaagtatcc acaaaataca ctcctttctt tttataagta atgtctaaat gacattcgag
     4381 gttaaaaata tataaaatta taatataaaa ctactaaatt aaagtctaaa tgacatttta
     4441 cttaaattaa tatgctcata atatgatttt aacatattat agttgaaaat atatggttta
     4501 ttttgatttg tatatataac aataggttta attgttataa aaatgtaaag gggtgtatga
     4561 atagattgta taaatttatt tcgataaact aagattgctt tttgattgtc tgtaaaagag
     4621 aaaaagatta agagaaaaat agtattatat tgtaatttat attaatcaat tacaaaaatt
     4681 ttatgaattt attctttagg ataaaatatt taagaataag ataaatttac aatataatac
     4741 tataatactc ttttatctag ttttattttc tttatagaac aataatatta taaatgctag
     4801 tagatttaca cagaatactg ttatatacat ctgtttaaat cctaagttta gagtagattg
     4861 tagtgtgttt tctatttttg ttctattatt ttctatggaa tttatataat ccaattttga
     4921 tttatcaaaa gcctttggta tttcagtttt tacctcttgt gtctttgcta aaactgcttg
     4981 gagtgatttt ttttgctgtt gcattgtatc aaggcttttt tccatgtctt ttttttggtt
     5041 tatagaatta tttaacttgc tttgtagatc tttcttttga gattccattg atcttagttt
     5101 tttacttaat tcgttctttt gagttttatt tttttgtaac tttgtattta agtcatctct
     5161 tgtctttttt agttgctgta tttgagcatt taaactttct ttttgttcat ttacatttgg
     5221 tttattctcg ctggaattgt tattcttatt ttctgtagaa tcattcttag tgtcgtctga
     5281 ttcagaatta ctggtattac tctggttttg ttcatttgag ttattttcgt tatcagtatg
     5341 agttttttct gtagtaggta ttttattata agcagttgta agttccttga tggcctcatc
     5401 ttgtttttca agaccttgtt ccataccttt tataccttta tcaacaccag caataccttt
     5461 ttttacacca tctattcctt gattaatacc agctctagct tttaccatat tgtcaatacc
     5521 agtttgaact cctttaatac cactatttag tttactttca ccgtcttcaa taccgtttat
     5581 tccactttgt gttccatcta ttcctttttg tacatttttc tgtattttag taattataga
     5641 tggaactttt tcatcaaaca ttgttgttga tatttctttt gttatattgg taatgttggt
     5701 tgtatctgct gactgaactt tattaagtaa ggattttggg agtttgccac ctgtcatgtc
     5761 catatttata tttgaagtat cattcatatt tgggatagag acatttttta aattttttgc
     5821 catttcaggg tctgatttta agtcatctat catgttttca agttcttcaa gttgaactat
     5881 tttaggagtt gagggctttc caactaagtc cataatatta tcttgcacag ataatcctgc
     5941 atgtgctata aatccaatca ttatagctgg agaaattgaa gtaccaattg agcgtatcaa
     6001 ggataatgtt gcaagtgctg aattagattc ttctaatctt gtatttgata acatcatata
     6061 gtttagagga gttcccatag taaatcccat tcctaatccc ataaatatta aacttacaca
     6121 tacactaaat acagtattgg tttttagagc aattaaaact aggtaaagag aacctatcat
     6181 agatacagaa aatcctagta atagtatttt ttttgcccca aatttgtcaa taagtcttcc
     6241 gcttaaagga gcagcaatac ctgcaaataa tcccaatata gctacaaaat atccaccaga
     6301 accagcattt atctttaatg cattttcagc atactgtgga acgaatacca tacccatcat
     6361 tccaatacca acaattaaag ataaaataag tactatcaat attctcggat ttaaaaaata
     6421 ttcaaagctt aaaatagggt ctttagctct attttcaata aatatgaaaa taggtataag
     6481 tacaataaat gtcaataagt atggataaac tgatatatct tgtatagaat ttttaaaatt
     6541 aaaaaagtct atattcataa gaccatataa tagtgatacg attatacata tcaacatcaa
     6601 tgtaccaagc ttatctattt tttctttaga ttcagactta ttgtttttaa tacagaaaac
     6661 tccaccaata agtatgatta aacaaattgg aagatttaca aaaaatagcc acttccaatt
     6721 ttgagttcca aagatactta gaattgtgct accaatgctt gaacctaaaa tattggcaat
     6781 accatatgtt gccccaacta aacctaaagc catgcctctt ttattttctg ggaaggtagt
     6841 tccaaattct gctgtagcaa ttggcatgat tccaccacca ccaattgctt gaataacacg
     6901 accaattaat aaaatataga aattggaaaa tagtgatgat aatccacata ttaatgaacc
     6961 actaccaaat agaaatatac taactagata cacatatttt cttcctattt tatctgctaa
     7021 ttttccagag ataggaatta cagaagcgta tgttaatgtg tatatggtaa tcatccaaat
     7081 tcccgttttt tcatttactc caaggctatt ttgtataatc gttcttgctg gtgatactat
     7141 tccagtatca atagctccca taaatatacc taatagataa agtgtaagta ctaaaaatat
     7201 ttttggatta ctgtccttgt tcttaatact agtattatga ttcaaggtaa acaccacctt
     7261 aatgtattaa tttttcaata tagggtttta aatcttcaaa ttctatatga agatatgttg
     7321 aatatgaaaa aaacattttt attatgatat tgcatactaa tttgtcatta ttaaaaaaac
     7381 tttctttttc taaagcttca tatactttat ttgaaagctg ttcatgaaag tcgtctttgt
     7441 gttttttcat ttcaaaaaat ctctctgttt caaaagtgtt taaaaagtca ttgtattgga
     7501 tattatgaac tttgtttata aaatttctta tttcagtaaa aataatgttt agttttttta
     7561 tgtaatcttc agtgttttta ttggagtatt caattcgcct tatcaataaa tcccattcag
     7621 attttgtgat atattctatt atttcttgtt ttgaattaaa gtaattatac actgtccctg
     7681 cacttatacc acatctttga gctattttcc ttatgctaat atttgagtaa tcttcttcta
     7741 aaatcatatc ccgagacact tttaatatat cttcttttac attttctaaa atctttggca
     7801 tattaccacc tcttttatta aaattattat gaacactgtt tattatactc ctttaaaatt
     7861 tattaatcaa tatgtaagct ataatgtata atattattat atagatatga tagaaaataa
     7921 gtgtcttctt actaaatatc tattatatta aatttgtact taagtatgat tatttattga
     7981 aatatgtgat attaaatttt tctttttaat aaattaaata ttatatgata aggagaatat
     8041 ggtgatggaa aagaagtatg taatggcatt agaccaagga acaaccagtt caagagctat
     8101 actatttaat aaaaaaggcg aaatagtgaa aattgcacaa aaagagttca atcaaattta
     8161 tccaaaagct ggatgggtag agcatgaccc tatggaaata tgggggtcac aaagtggggt
     8221 aatgagagaa gttatagaaa cagcaggtat aagaccagaa gaaattgctt caatagggat
     8281 aactaatcaa agagagacta cggttgtttg gagtaggtat actggtaaac ctatttataa
     8341 cgcaatagta tggcagtgta ggagaacgtc agaaatatgt gatgaactta aaaataaagg
     8401 attggaagaa tcaataaaag aaaaaacagg tcttttaata gatgcatact tttcagcaac
     8461 aaaagtaaaa tggatattag ataatgtaga aggagctagg gaaaaagcag aacaaggaga
     8521 gttattattt ggaactatag atacatggtt aatatggaat ttaactcgtg gaaaagtaca
     8581 tgtaacagat tactcaaatg catctagaac aatgatgtac aatataaatg atttagaatg
     8641 ggatgaggat atattacgag aattggacat accaataagc atgttgccat tagtaaaacc
     8701 ttctagctat gtttatggtc atacagatga gcgcatgtta tcaggagcta aaatacctat
     8761 tgcgggatgt gctggagacc aacaagcagc attatttggt caaaattgtg ttgaagaagg
     8821 tactgcaaaa aatacttatg gaacgggttg tttcttactt atgaatacag gcagtaacat
     8881 agttaaatct aagcatgggt tacttacaac aattgcttgg ggagttgatg gaaaagtaac
     8941 atatgcatta gaaggaagta tatttattgg aggagcatct gttcaatggc ttagagatga
     9001 attaaagata attgaatctg caaaagatag tgaaatgtat gcaaataggg tagatgatac
     9061 taatggtgta tatgtagtgc cagcatttac aggtcttgga gctccatatt gggatatgta
     9121 tgctaggggt tcaattttag gacttacaag aggagcaaaa aaagaacata tagtaagagc
     9181 cacattggaa tcaattgctt atcagacaaa ggatgtttta gaagcaatgc aaaacgattc
     9241 aaaattaaaa ctaaaatcat taaaagtgga tggtggagca agtaataata attttttaat
     9301 gcaatttcaa tcagatatat taaatgtaga tatagataga cccaaaatag tagaaactac
     9361 agctttagga gctgcttatt tagcagggtt gtctgttgga ttttatattg gaagaaatga
     9421 aattacttca aagtggtcag tagaaaaaga gtttaatcca aatatgagtg aagagaagag
     9481 atgtaaatta tataaaggat ggaaaaaagc tgtatcgaga gctttaagct gggaaaaaga
     9541 agatgaattg gatatttaaa aattaatcaa agttaaattt atttgaattt aaaaagagct
     9601 ttatgtaaaa tatgaagctc tttttttgtt gttaattttg catttttata taatcatact
     9661 aaattatata atttattttt cgttttcata taaaaacatt ggtatagaat tgtaaatgtg
     9721 ttggtcacca actactaaga gaatgtctcc ttctaaaaat tcaatatatg gtccaggaga
     9781 tattataagt tcttcacctc ttttaattgc aactattgta gcacctgtat tttgccaaaa
     9841 tttagtttgt gcagctgttt gaccaatata tttgcaatta gaatttatgg tgaactgaac
     9901 gggcactaaa ggattgatta tttcaagtct gttagaatag tctaaaattt tatttatagt
     9961 atctaatatt tcattttcta aattagattt agtatctatt aaattataaa gtgttgattt
    10021 atatgatgat atatttgtaa tatctctata tttttttata aaagaagaag ctttgtctgg
    10081 agatagtaca ataacacctt taccctttat gcttttaaca acgccaatgt cctctaaaat
    10141 tttgacagct cttcttatcg tttctggaga tacattgtat ttaccagcca aaacagagcg
    10201 tccaaataat actgaatcct cttgtatttc acccgtgtat attttatttg caatatccaa
    10261 cgctattttt tggtatattg gcatcgtata gtttttatcc attgtttttc ccccaataca
    10321 aaatttatgt taaaaaaata tataatctta acaatactat tgtataattt ataggtttat
    10381 atgtctacaa tatgtacaaa tattgtgcat gttttaaaat aatattttaa catgcattta
    10441 gtatacatga ttttattata cttaaaacta tataaagaat taattataaa aatataaaga
    10501 tattttactt acaaagaatg ataattatat agatgaaaga aacaaatgaa tgaagaaaaa
    10561 tgattctgtc ttgcatataa agtgattaaa aatgaaaaat actttaaaaa tgaaggttaa
    10621 agcccttatt ttaagtgaaa ataattaatt attaactaaa aaatggtaaa aagatttttt
    10681 gaaaaagaaa tacaaatgaa tgttcaatta aaataattgc aaaaatattc cttaaatggt
    10741 aaaattatat taattaaatg caatagaata gttgcaaaaa taaaattgaa tacaagtgat
    10801 aaaatatttc atgcaatata taaaataaac aaaatttaaa aattaaaact acttattcta
    10861 tcaaaatgtt agcgaggagg ggtcataaat tgtataaagt agatttaaat agtgatttag
    10921 gagaaagttt tggaacgtat aagattggtt tagatgaaga agttttaaaa tatatttcat
    10981 cagctaatat agcttgtggg tttcatgcag gtgaccctag tcatatggaa aaaacagtac
    11041 agctagctaa aaaaaatgga gtgaaaatag gagcacatcc aggtttccta gatttaatag
    11101 gatttggaag acgtgagatg aaaataacga agcaggaagc taaagattat acaaaatatc
    11161 aattaggtgc tttaatggca tttgcaagtt caaatggctg taatatacaa catgtaaaac
    11221 cacatggagc attatacaat atggcagcta aagataaaga gttagctatg gggatttgtg
    11281 aagctatata tgaagtcgat aaagatatta tactgttggg gctatataat agtgaaatga
    11341 taaattctgc taaagaaatt ggacttaggt ttgcaaatga agtatttgca gatagagctt
    11401 atgataataa tggttttttg gttccaagaa gcgtagaagg agcagttata catgatacaa
    11461 aacatgcaat agatagggtt gttaggatgg taaaagaagg aactgtagaa actttaacag
    11521 gtgaagttat acatatcaaa gcagattcta tatgtgtaca tggggacaat ccaaaagcaa
    11581 tagagtttgt caaagaaata agaaagcgtt ttgaattaga aggtatagaa gtttgtccat
    11641 tagaaaacat agaagtttgt tcatcagaga acatagtata agactgataa catgaggggg
    11701 agataaaata tgagtacaaa aaatattaca gataaaacta aaaataaaaa agatgttgga
    11761 gcacttatag gggctgcatt tataatggca acatcagcaa ttggaccagg gtttttaact
    11821 cagactgcac aatttaccca agattttgga ccgaattttt catttgtaat tttaattaca
    11881 acaatccttt ttataggggc tcaagtaaat gtatggagag taattggtgt atcaggactt
    11941 agaggtcaag atatagcaaa taaaataata cctggattag gatatttagt agctttttta
    12001 gtagctcttg gaggattagc ttttaatata ggaaatgttg gtggagctgc acttggtatg
    12061 aatgtaatgt ttaatatgaa tatgacatta ggaactgtat taagtggatt aatagccata
    12121 tttgtattta tgtcaaaaaa ttcaaattct ctagtggata agataactaa atttttagca
    12181 ttaggaatga taattatagt tggatatgta gcaatatcaa atcatcctcc tgttggagag
    12241 gcagtatcaa gaatggtaaa accagaaaat ccaaaaggat tgatattccc aataataact
    12301 ctacttggag gaagtgttgg aggttatata acttttgctg gaggacatag attaatagat
    12361 ggtggaataa caggagaaga aaatataaaa gaaataacta aatcttctct tttgggtata
    12421 ttagttgcca caatgatgag agttctttta tttttagcaa tactagcagt agtttcaaaa
    12481 ggtcttcaac ttgacccaga aaatccagct gcttcagctt ttaaatttag tgcaggagca
    12541 ataggatata aattctttgg acttgtatta tggtctgctg caattacatc agttatagga
    12601 gctgcttata catcagtttc tttcttaaag acactaaatc cttttataga taaatatgaa
    12661 aaatatttta taatagcatt tatagcaata tcaacactta taatggcatt tataggaaaa
    12721 ccagctactt tattaatttt agcaggagct ttaaatggat tgatacttcc aatcacatta
    12781 ggaattatgc ttatagcatc taaaagaaaa gatattgttg gagattacaa gcatcctaca
    12841 tggcttttaa tatttggatt aatagtagtt ttaatatctg catatacagg aataacttct
    12901 cttagcagcc taggtgcttt atttgcttaa tttagaagag gtgacattta tggaaactag
    12961 atacttgata tcaggggata aggcagtggt tgctgaattt ggaaatgaga tatcagaaga
    13021 tataaacaaa aaagtaattt catttatgag agcaatagaa atatcgaatc ttaaaggtat
    13081 tgtaacagaa atggtaccaa catataggtc acttatgata agttataatc cattagaaat
    13141 agattttgat agtttgattg agaatttaaa aaaaatagaa gataatttgg aaagtatagt
    13201 attaccaaaa cctaaaatac atgagattcc agtatgttat gatgaggttt ttggtataga
    13261 tattaaaaac gttgcaagct acaataatct cactgtagat gaagttataa aaatacacac
    13321 aagcagagag tacttgatat atatgctagg gtttactcct ggatttccat atctaggtgg
    13381 tatggatgaa agaatagcta cacctagatt agaagtgcca aggactaaaa tttatggtgg
    13441 gagtgttgga atagcaggtt cccagactgg tgtatatcct attgatagtc ctggtggatg
    13501 gcaaataatt ggtaggacac ctttaaaatt atatgatgaa aatagagaag aacagatttt
    13561 gcttagagct ggagatttta taaaatttgt tccaataaca ttagatgaat ttatagaaat
    13621 cgaaaaaaat aacttaagta tttaaatatc gttatagtac agggagtggt atatatggga
    13681 ttcaaagttg aattaggagg tttccaaaca ttaatacaag acagaggtag agttggctat
    13741 ggtcaatatg gtgtatctgg atgtggagca atggatgagt atgcacatag attaggaaat
    13801 atattagtag gaaattctga agatgaggct tctttagaag ttttaatgtt aggtcctaca
    13861 attacctttg atgaatatac gcaaatagca gtaactgggg gagatttagg ggctaaaata
    13921 aatggaaaag aaattcaaaa ctggaggtca tatcaaataa atccaggtga tgtattatca
    13981 tttagaggtg tgaaatcagg tgctagagcc tatgtgtcaa tagctggtgg aatagatgtt
    14041 ccacttgcaa tgggaagtaa gtcaacttat acaagagcta aaattggtgg atttgaaggt
    14101 agagcactta aaaaaggcga ttatataaat acttttattc aggaaaaaga ttttacaata
    14161 aataaaaaat taagttcaaa atatattcca acttatagtt cagaaatcgt actaagaatt
    14221 gtaaaaggtc ctcaatttga tgctttttct aacggtgaag tagagaaatt tttatcaaat
    14281 aaatacaaag ttacgaatga aatagataga atgggttgta gactagatgg agagagtata
    14341 aagcacctta atggagcaga tattatatct gatggtattt cttatggagc tatacaagta
    14401 cctggtcatg gaaaaccgat tataatgtta tcagatagac agactagtgg tggctatact
    14461 aagataggga acgttataag tgtagatttg tataagttag cacaagcaaa gccaaatgat
    14521 gtagtaaaat ttgaacttgt agatatatat gaagcacata gactattgag agagcaagaa
    14581 gataagattc aagatatcta caagagtatg aaaaatataa gagttgtaaa ggcaaaagta
    14641 ttaaatgata tagcagttta atttaatttt aacaaataca atttttatta taatttataa
    14701 tataacttac aaatagctat ttactattta ccataattaa gatggtttag attagtcaaa
    14761 tagctatttt tttagtttga gtaaatattt taaatttaaa taaatacttc aaatttaaaa
    14821 taaaagcaat atttaaatct attatattat ctacaaaaag taggtgtgtc atattgagtt
    14881 tatttagatt ttattggcca aattggaggt gatataactc catatccacg agctattgta
    14941 tctttacttg gattgatgat tttatgtggt acattagctt ttataaatgt tgagtcacct
    15001 ggatttaatc taactacctt ttcacccata caaatatcaa ccacaccact cgttaccacc
    15061 ataatttctt ctgatgcatg atatatataa tctttggcat cttcactttc tggtttaagt
    15121 tcaaactcta caaataaaat tgaaggtgta aactcagttg taggcattgg agatactatc
    15181 tcgtaaaaca tttcactctt tggaaatttc atcataactc tatcttcttt tctaatgatt
    15241 aaatcatcag tattattgtc atccataaac atatataaag gagtgtctaa aacttcagcg
    15301 atttttctca atgaagacaa ggatggttca atagcatctc tttctatttg agagatataa
    15361 cttatagata aatctgtcaa tttggaaaca tcacttaatg tttttttctt gctttttctt
    15421 atatttctca tcttttcacc tagcataaga acctccaaat aataaaatta atataaaata
    15481 ttcgcttaaa acaaagtaat tatagcacaa catagaagta gtggcaataa gaatacatag
    15541 tagattttga caattaaaat ttaattaaat ccaaataaaa gtctatgaaa atgaattatt
    15601 gttaaattaa tataaagaaa ataataataa aaaaatttaa tagaagagta ttaattgaat
    15661 taagattata acattatatt ttggaaaaga gtaaattttc agtaaattat cttgacatat
    15721 atattgaagt aataatataa tttaaagtat aaaattttaa aaattgaatt ataacttcaa
    15781 aaaataagga gggtaatatg aagataacag atatcaaatt tgaaaagctt agaattaaat
    15841 taaaaaaacc tgtagtagtt tcatttggag taattgagta tggagaaagt ataattttaa
    15901 aaattgaaac tgatgaaggg tattgtggat ttggcgaagc agcacctctt gcagcagtta
    15961 caggtgaagt tttggataat gttctttcag tattgcttat gtttaaaaaa gagttaatag
    16021 gcaaagaccc actagatatt gaaaccatac ataccatcat ggatggaatt attataggta
    16081 atacttctgc aaaagcagct attgacatag ctttatatga tattaaagga aaaattatga
    16141 atgcacctct ttataaagtt cttggaggat ttgatagtaa agtccaaacg gatattacta
    16201 taagtataga taagccagaa aaaatggcag tagaagcttt agaaagagta aaagaaggtt
    16261 ttaggatatt gaagcttaaa gcaggtataa atcctgaaga cgatattgaa gctgtaaagt
    16321 taattagaga agccgttgga gagagtataa gaattagaat tgatgcaaat caaggatgga
    16381 acgtaaatag cagtataaat actatcaaaa agttagaaga atttaatgtt gatgcaattg
    16441 aacaagcact tccacattgg gatttagatg gtactgctta cataagaaat aaaagtaaca
    16501 caaaaattat gatagatgaa tcattacatt caccaataga tgcaattaag gctataaaaa
    16561 agaatgcagt tgatacattt aatattaagc tgatgaaatc aagtggaata tatcctgcta
    16621 taaaaatcaa caacatagct gaggcaagtg gagtaaattg tatgttagga tgtatgctgg
    16681 aaacgagaat tggtataaca gcagcagcaa atttaatagc atctaaaaag aacataacag
    16741 aagctgacct tgatagtttt atgttttgtg aagaattaga aggtatatct ggaggatttg
    16801 taatggatgg agatattatg aatcttgtca ataaaccagg tttaggaata gaagtaaact
    16861 tatgataaat ttggaggtta aatatgaata cacttttttc aaatccaggt agtatacttg
    16921 ctgtaatgac atcaatgatt gcattgggtt tttacttaca aagatacaaa gccattaaaa
    16981 gcttaggacc agcacttaca ataatcataa tgggaataat actttcaaac ttaaaagtag
    17041 ttccagtaag tactgagtta tatggaacta tttcaacata tgctattcca gtttctatga
    17101 ctataatgtt gatgagtgtt gacttaaaag aaatgacaaa attatcaaga gaacctttaa
    17161 ttgcaatttt tgtagctgta ttaactgtaa gtatcatggc atttttattt ggattagtat
    17221 ttgcagaaaa aatatcagaa ggttggaagg ttgctggaat gtttgtaggt acatatactg
    17281 gtggaagtgc taatcttaca gctattggaa caggtcttaa tgtaagtaga caaactcttg
    17341 ctgcagcaaa tgcagcagac tatgtaattg gagtaccaac attgatattt atgtttgctc
    17401 taccagcaat attaaaaaat tcaaagaagt ttaaaaaact ttggccatat catgtagaag
    17461 aatcagaatt agaggattgt caaaatgaag aatttatgga gtcaaaagaa tggagtatta
    17521 aagatatagc ttggatgttg gctataggtt ttgttgttac agaagtttca acaatacttg
    17581 ctggatattt caattcaagt tttagtagtg cagcaagaat acttttagtt acaacaattt
    17641 caattataat tgctcaatta aagccagtaa agaaactaaa gggaaattta gatttaggat
    17701 tatttgttgc attattcttt ttatgtacaa taggtttttc agtagatata aaagagtttt
    17761 taggctcaac ttttactata acactttact gttttagcat aatttttgct tcatttgttt
    17821 tccatttagg cataactaga cttttaaaga taaagtatca atatgtaata ctttctatag
    17881 taggagcaat tgctgatggt cctacttcag ctttagtagc tgcttctgca aagtggaatt
    17941 cgcttgtaag tgtagcagtc gtaatgggag taattggtgg agtattagga aattatgcag
    18001 gtatatcagt ggcatatgca ataaagatgt ctctaggatt atagggggga gtttaattga
    18061 taaaatttaa attatacata tctggactat acagtggaaa tgtaatcttt gatggagatt
    18121 tattagttga aaagttgaat ccttttacaa ataaaataga aagtttaaaa ccaataagca
    18181 aagaagaaaa tacatattat ttaaatctta caaaaataga tttaaagtct ttatttaata
    18241 actttgatgt atatactaaa agcttagtaa acacagataa agacacagtt ataaataact
    18301 taggagaaac gcattctaaa cttaatgaat atatatgggt tcaaagaaac aaaaagttcc
    18361 cactagatat aattattgta gataataaaa tagtaggttt tatctgttta tctagagaaa
    18421 cttgtactat attaattatg gatggatatg aagagtatac tgtattaaaa gaatgggaaa
    18481 aaactcataa aaatgaagag atatattcta taagatttgg tggaaattat atgattgata
    18541 tgaaagatgg tataaaactt tctacagatg tatatctccc tgattttgta gattctgcca
    18601 aaaaagcacc gacaattctt atgagaactc catatggaaa agaaaatgat aaagaaatat
    18661 attataaata tgtacaaaga ggatatgctg ttgtaataca agatgttagg ggaagaaatg
    18721 aatcagaagg taagtgggaa cctatgattc atgagaggga agatggagat agtaccataa
    18781 attggatagt atcacaggaa tggtcaagtg gaatagttgg gatgcttgga gcatcatatc
    18841 ttggttatgt ccaatgggca gctgcatctt caggcaataa gcatcttaaa gctttagtta
    18901 gtatagtaac atcaggaagc ccttttattg atataccaag aaaaggagga gcttttgtat
    18961 ctggtatgct tgcatgggca tttatggtat caagaaataa agttgataga agcaaaatgg
    19021 taagagatga ttgggatgat gttttaaaca tacgaccaat agaaaatata cctgttgaag
    19081 ctcttggata tagaattgaa tttctggaag aatggttaaa gagagtagaa aaagatgagt
    19141 attgggattt gatggattgg catttacaaa aagataaaat aaatgtacct gcattggttg
    19201 tatctgggtg gtatgatgat aatagtatgg gaactacaga ggccttagat gttataaagg
    19261 actatgaaaa aggaaagaga aaggctattc taggtccatg gatgcataat tcaaatactt
    19321 taagagatat aaatggtatt tctttaggaa atagttcatt aagatatgat ttggattata
    19381 attatctatt atggtttgat agatacttaa aaggtataga aaataacata gatacgactg
    19441 ctccagtaga gtattactct gtaggttcta ataagtggaa gacagaagaa aattggccaa
    19501 taataaataa gatagataaa agtatgtatc taataagtga tggaaatgct aacacatctt
    19561 taggaaatgg tagattagta tttgataatg atttagaaga aaagtatgat agttatatat
    19621 ataatccaaa agacccatca gtacaactaa ttgatatgtc agagaatgaa gttggagttc
    19681 caaataacta caaagattta gaaaaaagaa gtgatatgtt gtgttataca tcagatgctt
    19741 ttagtgaaga atttactgta acaggtgata taaagttaga gttttttgca tcaagttctg
    19801 ccaaagatac tgactgggtt ataaaaatta tggacgtgga tttagatgga aattcaatta
    19861 aattagctga tggtatactt agtgctagat ttagaaatag tttttataag tcagagttta
    19921 tggaagaagg agagatttat aagtttacga ttataacttc aaaaatatca aacacgttta
    19981 aagttggtca taaaattaga ctagatataa cgtctagtgc taaaaacttt attttccaaa
    20041 atagcaacac gacagaggga tataatagta ttgaatatat agaagcaaag aatacgattt
    20101 atcatggagg taaatatcca tctaaattga tattgccaat agagaataag taaatttata
    20161 tagattaaat agtgaggagg tgtctcaaaa tgaatttttt agttcgtgag acactttctt
    20221 ttatacgaaa tcaaatatat tttcatcatt tcaacaatga aaaagaggct tatctctctg
    20281 ttttgagata gcctcctata agctaaattg attattttgt agctgcaaca acgctcattt
    20341 caactaacaa atgctctcta gcaagtctag cttcaacaca agctctagca ggttcaaatc
    20401 ctttttcaac ccaagcatcc caaacagagt tcatagcttc aaagtctttc atatctctta
    20461 aataaatagt aacagatagc aaatgttgct tatctgaacc atatttgttt aataaatctt
    20521 ctattttagc taaaacttca gttgtttgtt caattactcc tccttcagca tgagtttgtc
    20581 cacataaata aactgtattg ttatgaacta cagctctact cattcttcca gttccttcat
    20641 atctctttat ttccatattt taaatacctc catagttaaa aaatctatta aaaatctaaa
    20701 taataaatta aaaaaagtct atactatata aaattatatt atatattctg ataataatcc
    20761 ataaaaacat ataacttttt agctataatt attattgtta ttaattgaaa agatttatta
    20821 gttacttgac atagtttaca cttttaatta tagtataaat tggaatattt agataattat
    20881 tatttaatgt ataagctaag tttttaatta atatggcttt atacatttat atagaaaaga
    20941 ggtacgtatg agtagcacaa tcaaatcaaa tcgaataaaa ggtataatgt ttataatagc
    21001 atcagcgctt ggctttgcaa tgatgtctgc attcgtaaag ctagctggag atttaccaag
    21061 ttttcaaaaa gtgttcttta gaaatctggt atcagctata atagcttttt ggcttatcat
    21121 aaaacataaa ggaagtctaa ctggtaaaaa agaaaattta aaagtacttt tatataggtc
    21181 aatatttgga actctaggag ttatatttaa ctactatgca attgacaagt tgatgctttc
    21241 agatgctaat atgctaaata aaatgagtcc cttccttgta gtaatatttt gtgcaattgc
    21301 tctaaaagaa aaaataaatg taaagcagat aggagctatt ataattgcat ttataggagc
    21361 tttattcata ataaaaccaa catttagtgt agaagtaatt ccatatcttg gaggaattgc
    21421 aggagctgta tttgcagcaa tggcatatac ttgtttaaga gttcttggag atagagaaga
    21481 ttactataca attgtatttt tcttttcagt tttttcatta gttactgtag ggccaattgc
    21541 ttttgcagta tatgaaccaa tgacccttat gcaacttata tatctattac taggtggagt
    21601 atttgcaagt ctaggtcaat ttggaataac tctagcatat aaatatgcac ctgctaagga
    21661 aatctctata tttgactata gtaatataat cttttcagca attttaagta tattcttatt
    21721 caatgtctat ccagatatgt taagtgttgt tggatattta atagtatttt cggcagcatt
    21781 ttatatgttt ttatacaata agaagttaga taaattagac aaagaaaaga agaagtaaac
    21841 tagataaaag tgtttttata atttgtattg aatatttaag tctttgtatt aaaattaaag
    21901 aaaatttaat taaaaatgtt tggtatatta acttataaca gcagttaaaa ttgaaaaatt
    21961 ataactgctg tttcttttat taaaaatatc caaatgtctg taaaaataga ctataataga
    22021 gtagtataaa taatataaag gggataaatt aaagtatgga aaaagtgaaa gttgctgtga
    22081 ttggagcagg taatagagga acgtatgcat atgctccata tatttatgaa aattctgatg
    22141 tatgtgaaat agttgctgta gcagaaccta aaaaaggaag acgagaacta tttacacaaa
    22201 aatataacct agatagtaaa aatgtatttg aaactttaga agactttttt aaacatgaca
    22261 agatggcaga tgcagtaata atagctacaa atgatgatag acattatgat gtagcaaaac
    22321 tagctcttga aaaaggttat catgtactat tagaaaagcc aatgtctaat agtttagatg
    22381 gtttggtaca tatagatgac ttgtgtgata agtataaaga taagattttt atgatttgcc
    22441 atgtactgag atattctcca ttttataata aattaaaaga aattgtagaa agcaaaaaat
    22501 taggagagct agttagtata caatataatg aaaatatagg atattggcat tttgcacata
    22561 gctttactag agggaattgg agaaatagca atgaaaccag ccctttaatt ttagcaaaga
    22621 gttgtcatga catggatatt ttgttatatt tagtaggaag tagatgcaaa aaaatatctg
    22681 catttggaag cttaaagcat cttaataacc aaaatgctag tggagagatg gcacaaaatt
    22741 gtcttcagtg tttagttgaa aaaaaatgtc catattcagc aaagagaatt tatttagaaa
    22801 aagatagaag tataaataga gctgttcaca taaatccaac tgaagagaat ctattaaata
    22861 tacttaagac aagtccatat ggaagatgtg tttacagatg tgataataat gttgtagaca
    22921 atatggtaaa tatactacag tttgaaaatg gagtgacggc tacttttaat ttatgtgcat
    22981 ttactaaaga aaatggaaga actataaagt taatgtttag ccatggtgaa gttggtgggg
    23041 atttaaataa aaatgagata agaattaaag aatttggaaa aaacgaagaa atagttatga
    23101 atccatcaaa tcaacagaat atggtagagg aatatgatag aaatcttata gctgaattta
    23161 ttaaattagt ttcaaataaa gaactagaaa agggtagagt agcagcaaaa gaagctatac
    23221 aaagtcatgt aatggcattt gcagcagaat attctagggt cagtgatgaa gtagtgtata
    23281 tagaagaatt ttttgatagt gctaagcaaa tgactaagga gatagaagaa actatatttt
    23341 aattaataaa aatttattaa ataaaataaa gatagattaa ataatagaaa tacgaaaata
    23401 tatgttctat taaggaatct atctttttta ttaaggtttt ttcttatccc tatcaaaaaa
    23461 atccatacat tctgataaaa gataagaaag taaagcgaat aagatttctg tacttaaagg
    23521 aatcgttact gaatccataa aggtatcaat tatgttcata gctagaaaag ttagtataaa
    23581 atcaattata aattcgcaaa gtttatatat tgagtatggt aatttgttta catatctgaa
    23641 tatatctaaa atagtagtgc atagaaaatc aattggagtg gttatacaaa aatatattaa
    23701 aaagaaaatt aatacagctc caagagattt atattcaaat cctaagaatg ataagaagcc
    23761 aattcctata accgtgaaaa tacaaaaagt tataccaaaa aaaacagcaa aaaataaaaa
    23821 tacagaaata aattcgccta aaggaatcct tcttttatta cttatcttaa tcaactcctt
    23881 aatttatgat atgaaattta tataaataat aacattctta aaaattcata atagtcctct
    23941 gtttcaaata gtactgtttg agaatctatt tttttcatat ttggataaaa agaggcctta
    24001 aaagctttat aatgttctct aaattttatt tctatcttaa atttatctgg caattttatt
    24061 aagtgcctag ataaatctcc agataaagct tttttgacac ctttttcaat tttttttagt
    24121 gcaaggtttg gatggattga aattgaacca tttccaattc cttttgacac aggaacagtg
    24181 actatatttg gatttagttt ttttgcactc tcacaaagca tttcatctcc acttaaaaat
    24241 gctacaggaa cattataata tgcagcaata taagcattta tagtaaattc agatgctatt
    24301 tcatcattta ttttaaaata gtcataatca atatccatag tatgagaaag tggattacca
    24361 ttacaagtag ctccagagtg atagccggtg aatatacatg catcaaagct ttcatcaatt
    24421 cctgccatca taactagtgg gtctttggtc catcctctaa gtattttgac gttttcaggt
    24481 aaaatatttg gattaatatt tctaccactg tcatgtgcat ctttaacaag tatttcatgt
    24541 acaccagatt gagtagctcc atcacatgct gctgttatct ctttactcat ttgtgttcta
    24601 tagtgttgcg aaaattcaca ttcaagtttt gtttcatccc aatttacaat accacaagta
    24661 ccttctatat cagcacttaa atacaatttc ataatatata cccccttatg tttattattg
    24721 atgataataa cagataacag aaaatacata atcaataaag ggcataaaaa tattatgtct
    24781 tactattaac tattattaaa tctgtaactg aaaaataact aaattggtat aaaaaaatat
    24841 aacactatgt gtcatttatt tatatagttt tttatatctg atgtttatat aaaattgtaa
    24901 catataagtg gttacattta ctagtttaac atagttaaaa tagttaggta ttatttttaa
    24961 gttaaattga ttattataaa atcgcagaat aaaagaaaaa tttaatttat taaaatgcat
    25021 gaaatgtttt ttcctcaaca taatgctaaa ttaaactcaa aaagaaataa tagaaaaatc
    25081 ttatgtagct tagaaagaat gtgaatgatt taagggaaaa atagaaattt attatataat
    25141 attaggctgt attatataat acagcctaat attatatgta tatgtgacat aaaatcttgg
    25201 taagtgtaaa taaaataaat gtggaaaaca agttaatgaa atcaagaaaa aagtaattta
    25261 aaattcaatt tcatcttccc aattaaattc tcctgatttt tctacactct taggccagtg
    25321 actacaccat ttaggaacgg acggattttc aaccctatca acgcttggag tatatggctt
    25381 taagtcaata acaggactat tatcattagc atcaatatat ggtattttta ttatgccatt
    25441 ttcatggtcg atattgataa tttgtacagc agtcaatgct attggattgg gtcttatagg
    25501 tgaccttgtt gcaaatattc ctataacgtc tggtccactt ttataaggtt ttggggtttc
    25561 taaaatactt cttagttcag gatcatcaaa cccatcagcc caccaaaata catttaaatg
    25621 actgaaatcg tcgagttcat ttaatgcagg tacatacttt ttgtctaatt taataaagac
    25681 ctcttcgcct tgaatacata ttttacctat aggatttatt aatatttctt tcataatatt
    25741 acctccaact atttaattta tcatttgtaa ttttagggta taacataaca tcatgtgagg
    25801 gtcaatataa aataataaat aaaaatatta atagttagaa tgataataca ttttttacat
    25861 ggagtatgac accagtaata aggcaatctt cgtcaatatc aaaagtagag gtatgcaaag
    25921 gggaagtaat gtttttttca cgatttgcac aaccaaggtg gtaaaaagct ccttcacagt
    25981 gtcttagaaa atacgaaaag tcttcagccc caagagtggg cgagtcttta aggataatat
    26041 tttcttctcc aaacaattct acagcatttt gtttgacgta atctactaga tggtttgaat
    26101 tgataagagc tggataacct tcctcaattt caacagaaat acttcctcca aatgttttac
    26161 aagtaaaatc gcaaatttct ctaattcgtt tttttgaaaa ctctctagtt tcaggagtca
    26221 aggttcttaa agtacccctt ataaccactt tatcacaaac tatattttcc ttgattccac
    26281 cttcaatttt tcctagagaa ataaccacag aatcagttgg atttgtgttt cgactaacga
    26341 ttgtctgtag agctgtaata atatgagcag atatgacaat agcatcaata ccttgatgtg
    26401 gataagctcc atgacatttt gaaccatgta cagttaattg aagtgtatcg gtggaagcat
    26461 ttagagtgtt gtattttaat tcaattagat ttttgttgat attgggattt acatgaagtc
    26521 caaatatata ttttacattt gggttttcaa gacatccatc ttctatcata agttgagccc
    26581 caccaatagt ttcctcggca ggttgaaaaa agaattttac atttacattt aagtaatcct
    26641 ttaatttaaa tagtatatta caagtaccca gtaaaatagc agtgtgtgca tcatgaccac
    26701 atgcatgcat tttgccaata ttaatagatt tataagaata attaagttct tctgttatag
    26761 gtaaggcatc tatatcagct cttatagcaa ctgtttcaaa gtttggactc acgttaatgt
    26821 atgcagttat ccctgtatga ttcttatatt caatataatt tatacctatt tcatttaagt
    26881 atttaataat ttttttctta gtttgaaatt cttctaatcc aagttctgga gtcttatgta
    26941 aatctcttct tatatttata acccaatcat ttatctcatg acaaagagtt tttatattaa
    27001 gcataaaaca catcctaact atattaaaaa tttatttttc ttcattaatt atattatttt
    27061 atgtaaatca ttattatgta tcaagtacaa ataaaatatc ttataattat attagagata
    27121 tttagtatgg agaagaatat atcattttat aagaagaggt gtttaaatgt taggaataat
    27181 tgcgccatct gggccattaa gaaatacaag tttggaggaa ataaagttta atttagaaag
    27241 ttatggttat gaagttaagt tttctgaaag ttgtagttta aattataaag gttatttagc
    27301 tggtaatgat gatattagag caagggatat agaagatatg tttttagata aagatgtaga
    27361 tataattatg tgcttaagag gaggttatgg aacaacaaga atattggata aaataaatta
    27421 tgatataata aagcaaaatc caaaaccatt tataggtttt tctgatataa caggtttaaa
    27481 tttagctttc tataaaaatt gtggtctttt accatatcat ggaataatgg cagcaaatgt
    27541 aggtaaatgg gataaattta cctataaatc attggtaaat gctttagaat ttaaagatga
    27601 attatattta gaaaatccaa aagaagaaaa gatttatact gtatgtgaag gtaaagcaga
    27661 gggaataata atgggaggaa atttatcctt aataattgcc acaatgggaa caaaatatga
    27721 aatagatgca aaagataaaa ttttgtttat tgaggaaatt ggagaacctc aatataaatt
    27781 ggatagaatg ttaacacaac tatattcttc aggaaaactt gaggaatgta atggtataat
    27841 ttttggagat tttaaggatt gtattgaaga aaatgatttg atggaattac taatagaatt
    27901 tgcaaataag gtcaataaac cttctatata taatcttcag tcaggacatt gtatcccaat
    27961 gataactatt cctttgggca gaatgtgcga attagatgct acagaaaaaa tcgttaaatt
    28021 aaaaaaatag tgtgtatata aatataatag aaatatggta taatcttata caagtaaaaa
    28081 tttagctagg aggatgaaaa atgtctgatg atagaataat agactttaac gaactaaaga
    28141 acaaagttaa agattctgat gtagataaat ttgagcaata catatataac ttatattttt
    28201 ctgttatgga tggaaagatg tctatggctg agttttcaag aaaaatattc gattatatga
    28261 gagataacaa catatctcaa gaaaaattta tgaaaataca aaagcaattt atggagagat
    28321 atggtatgga cactgaagaa gtagaaaaac aacttagaaa ctttggaatc gacccttcta
    28381 cagcaggttt tatgagtaat aatacttcca gcaaagtatc tacagaagat ttagagtcat
    28441 ttaagaagag tgcaggattt tatgagaaat atggagaaaa aatccaacca aagagttgta
    28501 taacaacatt tataaaaaat gacttaaatg atattaatgt aataatcgac caagaaaaga
    28561 taatgctttg tagtgataga aaaataaatc ttatggattc agaactaaat gagtttttat
    28621 tagaatataa gaatatgttt aataaaaaaa taaaggtagt aatgtgcgaa actacgaata
    28681 agtatgatta ttaaaaaatg aatttaaata ctgaagtagt atcaattaat tgtactactt
    28741 aaaaataaaa atcgcaattt attattcctt ataaatttgt ataaggaata tctaaactgc
    28801 gattttttat attgtgaaga ttagtcataa tagatagtgt tttaaattgt aatcttctca
    28861 ctaaaactgt gtttttctat tagaaatttt attaaaaata aatatctgat aattgtatta
    28921 tatgggatgt aaagtattag aggtttaacg aatctctttt aatacattaa ctccattttg
    28981 aatcatactg ttgaaaaata ctaagtttaa aatctcagca gggtgaacgt taggaatatc
    29041 ataagtatct actaaattca taggtacaac tatatttttt tcaatattat gttgattaaa
    29101 atatgatttt agagttattg caaattgata tatacatata tctgtgcaat cgccaactat
    29161 aattatatta tctatattat caaaatccaa attttctaat gcaaaaaaac cattagtaga
    29221 atttttagga agaatttgta ggttttctat cgacttaagt tcatcaacca attcactttc
    29281 tacatcattt tccaaacagt gaacaggata actcaaaagt tctattgatt taggagtatg
    29341 agaatcagta aatgctatta ctctatttaa tttgtttgat attttttttg taaacatttc
    29401 aattggcttt attaaagact ctactctagg agagtataat gcaccttgtc ttgcaaagcc
    29461 attattaaca tcaattataa ataaagcagt tttactcaaa tcataatttt ctattttttc
    29521 tattggtaga ttatctaaat tactctttaa agcttcaagt tcatttaata agttatccat
    29581 agttatacct ccaagataaa tttaataaaa ttgtacatat taattatata aaagtcaagg
    29641 agtacaaact attgtataat tagctgtatt gttttgagtg taaaagattt gaagcaatat
    29701 ctggatagtt agtaaaaata ccatctacat tgagttttag acagtgtagt atatcatcct
    29761 cttcatttac agtatataca ttgacaccaa gtagattttt gtgacaaagt tcaattagag
    29821 attcatttac taaaactaga ggtggatgaa gataactatt acccagaaat ttagcaaaat
    29881 tgactacatt gtttataggt aaataacata ataatcctgt ttttatacta gggtctaatt
    29941 tatgaaagtt ttttaaacta ttgctgttga aactagatat aagaactcta tttttcatat
    30001 tatactttaa aataagctct aaaacgtctt tttctatatt gggatagttt attttgtcag
    30061 tttttagttc tatatttaaa ataaggtctg taggtgaaat tagaattaat acttcttcta
    30121 aagttggaat tttacattct ttattgcttt gatatccttc aaaagatgag tttagagttt
    30181 ttaattttct taaagtaaag tcttttacaa aaccatgtcc attaaaagtt ctatcaacct
    30241 tttcatcatg aataactaca atttttccat ccttagttct gtggacatca agttctattc
    30301 catatatatc catatccaaa cattttttaa aagctagaat agtattttct ggatactttc
    30361 cactaaaccc cctatgagca taaatattca tttatatggt tccctcctat aagtataact
    30421 tatcttataa aaatttaatt aatataatat atcatattcc aaaaggtatt gttatatatt
    30481 agttaaataa ctatttgtaa tttatatatt atataaatgt agaaaaataa aatcatatat
    30541 gtgttataat atattttaaa cattgtaaat attacgaatt tagtagtcat aataagtgta
    30601 attttatgca agctcatgtt tttagagctt gcatatgtat tattaatata tttatatatt
    30661 gtgaaaataa aattccaaaa gaattttttc tatagactta gtagtaaggg ggaattttga
    30721 tatgtatgaa gttaatttag aacgagctat gaattattgg atggatttgt tagatgatta
    30781 tacgaatata gcaaaaattc caaatgattt tttttataaa gattgttctg atgaagtagt
    30841 agatgaggtt tttaagatga atattgagaa acatgaaagg ttaattcaat attctaaagt
    30901 gaacaatatt agcatagact ctatcataga aactatatat ggccttatac ttcaaaagta
    30961 tatatatgaa gatgatgtag tttttggtaa aacaatgaat ataattccaa taagagtaag
    31021 agttagacaa agtgaaaaat ttatagatgt tgtaaggaag ctaaaaaggc agtggagcag
    31081 gtctttagag tatggatact atttgttatc agatatagag aataaaaatc atctaaatac
    31141 taagcttata aacacaatat ttatagctaa aagttttgat ttatcatatg attatgacat
    31201 agaaaaaaaa ggatatgatt tatctgtaat agtatatact aagagggcac ttaaaataaa
    31261 aataaagtat aaaccatcta tatataaaag agaaactatt agaagaattt taaatcaatt
    31321 tgattatata atagagcaaa taatagaaga taatgatatt ttaatagaag atttagaatt
    31381 tgtatgtaag gaagaagaaa aagtattatt ggaagacttt aataataatc aatatgaagt
    31441 accctatgat aaaagttatg ttgatttatt tagggagcaa gttaaggtaa cacctagtaa
    31501 tatagctgct agggatgaaa atagaaagtt aacatataaa gaattggatg aagtaactga
    31561 taaacttgca ggttatttaa atcatatagg agtggaaagt gaagacatag tagctgtaat
    31621 gttaccaaga gatattaaca taattattac agcaataggg attatgaaat cagggggagc
    31681 attttttcca atagacacaa gtaatccaga agaaagattg aattaccttc ttgaagatag
    31741 taatgcaaaa gtagtgataa ctacagatga actcaaaagt aaggttgtaa atgagaatac
    31801 catagtactc gatataaatg atgaagaaat gtttaagctt ggatatgaat taactgaaaa
    31861 aataacatca tcaaattgtg catatacgat atctacttca gggtcaactg gtaggccaaa
    31921 gaccatagct atagaacata agagtttagt aaatatgtgt tattatagcg taaaatctat
    31981 aagcgctaca gaaaatgata tatgtggtat atacttaagt ttcagttttg atgctgtaat
    32041 gaaacaacta ttcccttatt tgcttgtagg tgcatcaata gatattatgc ctgaagaagc
    32101 taaatttgat gaatatacag taaatgaata ctgtgaggaa aatgacataa ctatactagc
    32161 attgccaaca gcatttgcaa ggttatttat acaaaattgc aataataatt cacttagagt
    32221 tgtacagact ggaggagaaa gattaaaagg atatagaaag cgtaattatg aactatataa
    32281 tgagtatgga ccaaccgaat ttactgttgt atcgacttct tttcatgtgg acagagaata
    32341 tggaaaaata ccaataggaa aacctatatt taatacttat gcatatgtat tagataaaaa
    32401 gaacaaacta tgtccaattg gagcaccagg agagctatgt ctatcaggca ttcaaatatc
    32461 aagaggatat ttaaataaaa aagggctaac agagcaagta tttgtagaga atccttacaa
    32521 aacttgtgaa cataataagt taatgtatag aactggtgat ttagtaagat ggttagatga
    32581 tggaaattta gattatattg gaaggatgga caatcaagta aaaattgatg agtttaggat
    32641 agagctatat gagattgaaa atataataaa taatattaca gaaattaaaa gtgttgtttg
    32701 tatatcgaga acaaatgatg atggagatat gtatatatgt gcatattatg taattgatga
    32761 ggaagatagt ggtaagataa atgaaagaac tattagagaa tatttaaatg agcatctacc
    32821 tccatatatg attccaacaa taataatgag gatagataag ataccagtta caccaatcgg
    32881 aaaagtaaat aaaagagcat taccagaata attttttatt atgataggtg tcataaagtg
    32941 atttcagaca tttctttatg acacttattt ttataaaaga atcaatttgc ataaaaacaa
    33001 gaattttctg tatatattta ctatggtgaa aatttattta taagaatgat tattgtattt
    33061 tttatacttt tccgtgtttt aaagcatttt ttttacaaaa ttaattttac at
//
LOCUS       DAEORZ010000032        33095 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEORZ010000032 DAEORZ010000000
VERSION     DAEORZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33095
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.032"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(103..507)
                     /locus_tag="KRH39_001381"
     CDS             complement(103..507)
                     /locus_tag="KRH39_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG8511298.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNYVKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLKSNEELKSVKYPPIYLKYKPDK"
     gene            complement(697..1884)
                     /locus_tag="KRH39_001382"
     CDS             complement(697..1884)
                     /locus_tag="KRH39_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBG8511299.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNKVLYYSFNEFRDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPRLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQSFIEIIRMIFGIRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIESLGNIPNNF
                     KVKNSVEQITILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVANRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            complement(1916..2341)
                     /locus_tag="KRH39_001383"
     CDS             complement(1916..2341)
                     /locus_tag="KRH39_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8511300.1"
                     /translation="MINENLKSLRKIHQYTQEELAEKLNVSRQSIAKWESGESIPDIG
                     SCIKLAKLYKVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVPKEE"
     gene            complement(2768..4717)
                     /locus_tag="KRH39_001384"
     CDS             complement(2768..4717)
                     /locus_tag="KRH39_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG8511301.1"
                     /translation="MERKYQNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMIHGGHGWLINQFLSPYFNKRTDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGIEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPIATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVVPVLKSKKVL
                     IAGGGPGGLQAAITAVKRGHKVILCEKTNELGGILKGEQALPFKYEMYELGNTFGKIA
                     KDLGVEVRLNTTVTKEYVDNENVDALIIAVGSEPLVPPIEGLNGENVVIVNDYYLEKE
                     KVTDEVVVLGGGLAGCEAAIHLAQEGKTVHLVEMRAELAPDANIRHRPILLEEIERQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDTLLDCAPYVA
                     QIGDCVRASTITNAVYQGHHAALDI"
     gene            complement(4689..5987)
                     /locus_tag="KRH39_001385"
     CDS             complement(4689..5987)
                     /locus_tag="KRH39_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HBG8511302.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEYLNSLAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLIFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVTNW
                     IINKYGVTSAFKSIGLIFLIIVCVSSFFIKKCPADFTPEGWVAPEKKGKVRVIINKNW
                     KEMLSTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDAAIVVSILALFNTF
                     GRIVAGYISDKIGRINTLSGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYIGPAVMKNVYSVDGNYQRAFIIAIII
                     ALIGFILTFLYRYINKIQNLNIENLMKGDIKDGKKISKSM"
     gene            6239..6796
                     /locus_tag="KRH39_001386"
     CDS             6239..6796
                     /locus_tag="KRH39_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG8511303.1"
                     /translation="MRTLKYAILGLLNRNPMTGYDIVKEFNFQLAEFWNAKHSQIYPE
                     LKKLVNEKLIVYDIKISGDVLEKKLYTITEKGKDEFLKWLEKDEPMERTPKDIFRLRM
                     YFSNNLDIDTRLILLEHQLLQHQERITFLYSQMERYSEVPPLDSNDFGDYIVLDGAII
                     REQGAIKWLTNCINHHKNLDNTTLK"
     gene            7240..7989
                     /locus_tag="KRH39_001387"
     CDS             7240..7989
                     /locus_tag="KRH39_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG8511304.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISV
                     DKPFIFVCPTYAWRLPIVVTDFIKKTKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENKFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(8150..8515)
                     /locus_tag="KRH39_001388"
     CDS             complement(8150..8515)
                     /locus_tag="KRH39_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG8511305.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(8531..9307)
                     /locus_tag="KRH39_001389"
     CDS             complement(8531..9307)
                     /locus_tag="KRH39_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG8511306.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            complement(9754..11181)
                     /gene="ascB"
                     /locus_tag="KRH39_001390"
     CDS             complement(9754..11181)
                     /gene="ascB"
                     /locus_tag="KRH39_001390"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG8511307.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            complement(11242..13170)
                     /locus_tag="KRH39_001391"
     CDS             complement(11242..13170)
                     /locus_tag="KRH39_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8511308.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINSAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIV
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            complement(13315..14157)
                     /locus_tag="KRH39_001392"
     CDS             complement(13315..14157)
                     /locus_tag="KRH39_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG8511309.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            complement(14671..17172)
                     /locus_tag="KRH39_001393"
     CDS             complement(14671..17172)
                     /locus_tag="KRH39_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8511310.1"
                     /translation="MIFHEVYKIYSKNSLKNNKLLKISCIISIVFATAILLFTRVLTD
                     TYSSEVKRNMAITNGGDIKIQNIEYNKYYFTSEQIDKLEELKDKEAIDFQLGSSMNTN
                     ILSNGMIESTELTVVNNIKMQNTYLKLRNEKDIVLSQKTANKLNVNIGDKVFLKLHSS
                     VYDDTYFKVSDIIPKKFSLSTAQKEAEVGQEVVGESYVYLPNETKYNVAYINIINKEN
                     VKNIKNEIKNVFKNHFEVRTTDELEQSIIKRISMQLDSINLIGAISLLITGICLCSTF
                     IVFILNRIHDFSTFKALGIKKRSLSFLVFTELMSITIKGIIIGIILGIPITIFYINLQ
                     HINLFNISILSIIKNIFISIVIIIIETSIFSLIPISFVKKIPYEGINRDNSMLSSYKF
                     NFIPDIILVIFLTSISFTIYVKSFLGIFFILGLILLDLLVYSLIYALLKMVCLIKKII
                     KGKYSITFIYLNNDCKTNAFSSSLLTITIIFVLLLFMISEITFAMFSNYEVDNSIKNS
                     IVYQTTLEGKLITDKKLIENDIDYFQYYPIKIDSIESVNNISVNDYIEKIPYENRNDV
                     MNDLNDLSVDLFNTKQTILKSDLEYGNWFKENDKENSIIINNYLHRYLIDYKLGDTVK
                     LRINGKKIDFKVIGFLSPKSLNKGVDLGYININENISPKTLDLSKNQPVIYFFKEKLD
                     NTLLSKLLHNDKYANLEDYNDFAYSMKTYVDDQKSLLLNIIFAVSLSSILLILTSQII
                     IFIRRVLDYQIMYRVGMSKRKLIKIGLLEMIIVSIIQSILIAIFYELFRFLLISLLQR
                     AYNINYLLILIEFLVVLSINCFIFILAQRNIKLDL"
     gene            complement(17184..17843)
                     /locus_tag="KRH39_001394"
     CDS             complement(17184..17843)
                     /locus_tag="KRH39_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003410138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8511311.1"
                     /translation="MIIEVSNLSKVVDLLNTELTILRNIDLYIEEGEFVSIMGASGSG
                     KSSLISILSGLDKNYTGNVKVCGENLSKLTDDELAEYRNRKIGIVFQSFNLITSMSVE
                     ENIKLPLIFSDNKDYKIVDKMIKKVGLTSKTKNNIKQLSGGEKQRVAISRALVCSPNI
                     LFADEPTGSLDSTNSHAIMKLFKNINNEYGTTIVMVTHDIQMAEYSDRIISIKDGTII
                     K"
     gene            complement(18414..18566)
                     /locus_tag="KRH39_001395"
     CDS             complement(18414..18566)
                     /locus_tag="KRH39_001395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511312.1"
                     /translation="MEKKFDTYIPTTGRWSLGCTIGFIMYKIVHVKVISPLGWWLINN
                     MINRIV"
     gene            complement(18757..20160)
                     /locus_tag="KRH39_001396"
     CDS             complement(18757..20160)
                     /locus_tag="KRH39_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG8511313.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKEGIGKLRKCVK"
     gene            complement(20199..20870)
                     /gene="pgmB"
                     /locus_tag="KRH39_001397"
     CDS             complement(20199..20870)
                     /gene="pgmB"
                     /locus_tag="KRH39_001397"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG8511314.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLERGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINSANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLA"
     gene            complement(20872..23262)
                     /locus_tag="KRH39_001398"
     CDS             complement(20872..23262)
                     /locus_tag="KRH39_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG8511315.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGIISEHSRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMKKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            complement(23288..24736)
                     /locus_tag="KRH39_001399"
     CDS             complement(23288..24736)
                     /locus_tag="KRH39_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG8511316.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            complement(25141..26028)
                     /locus_tag="KRH39_001400"
     CDS             complement(25141..26028)
                     /locus_tag="KRH39_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8511317.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            complement(26128..26577)
                     /locus_tag="KRH39_001401"
     CDS             complement(26128..26577)
                     /locus_tag="KRH39_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8511318.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            complement(26567..28474)
                     /locus_tag="KRH39_001402"
     CDS             complement(26567..28474)
                     /locus_tag="KRH39_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8511319.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQKEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDELMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            complement(28742..30637)
                     /locus_tag="KRH39_001403"
     CDS             complement(28742..30637)
                     /locus_tag="KRH39_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8511320.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(30673..31551)
                     /locus_tag="KRH39_001404"
     CDS             complement(30673..31551)
                     /locus_tag="KRH39_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBG8511321.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILKAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVETLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(32094..32264)
                     /locus_tag="KRH39_001405"
     CDS             complement(32094..32264)
                     /locus_tag="KRH39_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511322.1"
                     /translation="MSALLYGVCNKPISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            complement(32661..32873)
                     /locus_tag="KRH39_001406"
     CDS             complement(32661..32873)
                     /locus_tag="KRH39_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8511323.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVKNLFEFIEEE"
BASE COUNT        10910 a         5649 c         3428 g        13108 t
ORIGIN      
        1 aattatatgt ttatatataa aatcttaaat ttaattttat ttaaacactc tagtgatatt
       61 ttcaaataaa ttttaataaa aatccttcta ttttgtcata aatcatttat ctggcttata
      121 tttaagatat attggtggat actttacact ttttaattcc tcattacttt ttaaaagttt
      181 attattattt tccatatcat aaatttgtaa cattacttta tttacatata gagcataata
      241 attatcacta taaaaatatt gatactcata tcgagggtca tctttgtaat acactttgta
      301 tagtattcct tcataacttg ccttcttggc tttttctttt gtaatacttt ttatattatc
      361 ttttgaaata tcttgcttta ccatatactt atcaatatat gtttcagcat ctttccatgg
      421 tttaacgtag tttttatata caacaaaccc tgttacactt ataataccta taattaatac
      481 tataaaaata actttatatt ttttcatatt aatcctcctt catttattat ttaaaacttg
      541 atataatgca ctaaaaaaag gatgttatct tttttattaa aatatagcat ccctcatatt
      601 taacatcaat atatatgccc caactggtag gttaaaagac cccactgtca gtactttgat
      661 taaattacat gtatatatat ttttttaaca aatctattat atgtcaactt gtctactatt
      721 acatataatc tgtaatatta catctgctgc tttttttgag cctcctgcat tataaaagct
      781 ttttgatagt ttagttgcat tttctttata ttcattgtca tttataactt gaaatactgc
      841 ttttttaata ctttctgact tatcctcttt taacataatt cctgctccca aatgaacaac
      901 tctattagct accattctct gctctgaatg ttgaggaaat aataccatgg gtactccata
      961 atataaactt tcatttacac tattcatgcc agaatgagta acaaacacat cagttttttg
     1021 aagtatagta atctgttcaa cactattttt aaccttaaaa ttatttggta tatttcccaa
     1081 actttcaata tcagtacttc taccaacaga cataatcaca tcaacatcag aatctttaaa
     1141 tgcatttata cagtttttat aaaagctaac atttttatta ttaattgtac caagcgaaat
     1201 atatattagc tttctttttc tctctttagg ttcaataatc aactttgata ctgatggacc
     1261 tacaaatgaa tacttactag agaaggtttc taccattggt tgaaattctt ttgatgtata
     1321 tacaatagta tccgtatcat tatcattaga aactatagaa ataaaatttt ttacctcata
     1381 cccttttctt tgtaaaagct ttattttttt attaatacgt cttataccaa aaatcatacg
     1441 tataatttct ataaagcttt gtttcatcaa ctttgctgta tgtttattaa atgcaaaagt
     1501 tgttgtagag catacatatg gaatattaag tttttgtgca aataactttc cccaaaaact
     1561 taatgaatct gatacaatgc aatctggatt aaaatcttta agttctctac aaactttttc
     1621 atctaaagaa atagtagtat ctactatcat ctcaattaat ctaggaaaat ccttaccaat
     1681 ttttttctca tctccaggaa gtaattctgg aaggtattta tcacaacaaa taaattttgc
     1741 tccagcacct tcaattttat ctctaaattc attaaaagaa taatacaata ctttatttcc
     1801 tctatccact aattcacgaa ctacttcaat agtaggatta gtatgaccat atgcaggaat
     1861 gctaaaaaat actattttac tcatatttca tacctccttt tataattttt taaaatcatt
     1921 cttctttagg tactactccc acatgaccta taaaatttat caaatcacgt tgatgaacaa
     1981 ttcctaatcc tctttcctca tctccaagta ccaataattt atctcctgca tttatattaa
     2041 atacttctct tgcttctttt ggaatgacaa tctgaccacg ttcaccaact actactgctc
     2101 caaagaaaaa ctttccttta ggaggtacta taatacctgt tttttcttcc gaatgattaa
     2161 ctaagtcatc tagtttgacc ttgtacaatt ttgcaagttt aatgcaacta cctatatcag
     2221 gaatagattc tccactttcc cattttgcta ttgattgtct agaaacgtta agtttttcag
     2281 ctaattcttc ttgtgtatac tggtggattt ttcttagact ttttaaattc tcattaatca
     2341 tatttttcat acctcctatg tttataatat tggatttatt atttaaattc tatcaactaa
     2401 tcttaacatt catatatata tttatatact ttttattaga taaatttatt gtttagataa
     2461 catacttcct taagttataa taaataaaag caagcacaaa tctatcaacc tttgctaaag
     2521 tatttaagtt agggtcattt acattgttaa atacttctat atcttaaaaa gttttactca
     2581 tcaatcaaat gaaaacttgc aataagaatt aaaattataa taacaaaggg gctgtctcaa
     2641 aataaagata atcttttttc attattgaaa tgataaaaat atatttgatt tcacgcaaaa
     2701 aagtgtctca tgaactaaaa agttcatttt gagacactcc ttttttataa taaaacaata
     2761 tttttaacta tatatctagc gcagcatgat gcccttgata tactgcattt gttattgtag
     2821 atgcacgaac acaatctcca atttgagcaa catatggagc acaatcaagt aaagtatcta
     2881 ccacttctct tcttggtttt tgtccaagtg cacatattac tgaagttcct ggaatattta
     2941 ttttattccc agatttattc atacatacta caccttctgt tgttacactt aaacccttat
     3001 gttctgtata tacatatata ccttgtctct ctatttcttc taataatatt ggacgatgcc
     3061 ttatatttgc atccggggca agttctgctc tcatctctac taagtgaaca gtcttccctt
     3121 cttgagccaa gtgaattgca gcctcacatc ctgctaatcc tccacctaaa actactactt
     3181 cgtcagttac tttttctttt tctaggtaat aatcattaac tattactaca ttttctccat
     3241 taagaccctc tattggtgga acaagaggtt cagaacctac tgctataatt aatgcatcaa
     3301 cattttcatt atcaacatat tcttttgtaa ctgttgtatt taaacgtact tcaacaccta
     3361 aatcttttgc tatttttcca aaagtatttc ctaattcata catttcatac ttaaatggaa
     3421 gtgcttgttc tccctttaaa attccaccta attcattagt cttttcacaa agtattactt
     3481 tatgcccacg ctttacagct gtaatggctg cttgcaatcc tcctggtccg ccacctgcta
     3541 ttaatacttt tttagatttt aatactggaa caacttcagt tccatctaat tcacgtccaa
     3601 taagtggatt aacagtgcaa cgtcttgtag aagtggtagc acgttctgcc atacaggtaa
     3661 agcatcttag gcatttcact attttttcat cttgattact cataactttt cttggtaatt
     3721 cgtggtcagc tagtaatgca cgagccatgt aaataacatc tgcttttcca ctagcaatta
     3781 tctcttccat catttctggg tcatttaatg ctcctatagt agcaattgga atatttacat
     3841 gttttttaat ttcttctgca agatatacat tacatccatg ctctaagaac atagaaggat
     3901 gtgttactgc aaaccctctt tggtatgtac ctgctgatac atgaagtaaa tctactcttg
     3961 attccaataa tttagcaatt tctattccat aatctaaatc ataccctccc tcaaataatt
     4021 cagaaccact cattctaaac tctattggaa atcctggacc tactgcttta cgaacactat
     4081 ctaaaacttc tttagcaaaa cgaactctgt tttctaaaga accaccatat ttgtcagttc
     4141 ttttattaaa gtaaggtgat aagaattgat tgattaacca gccatgtcca ccatgaatca
     4201 taatcatctc aaaccctgct ctcttagcca atgctgcaac atttccatat gactctacta
     4261 tttcagcaat tatttcttct gtaagttctt ttatctctaa tccatcagga cgaacactag
     4321 gacttacccc ccattgtgat aatcctcgct tcttatcttt gtcaactaaa taagttcctg
     4381 aatattgtcc tgaatgagat agttctacac ttggtattgc tccatgacga cgaattgcat
     4441 ctgctgtata agtaaaactt gctaaggaat caactatttt taagtctaga tgatatgcat
     4501 gtgaaccatc tgtttctgga tgaaccatac attcactaat tgtaactgca cccgctccac
     4561 cttttgctct taattcgtaa aaggctgttg attttgagcc aattgtacaa tctgctgtta
     4621 tgtctgttcc ccccataggt gctgaaaaca ttctgttttt gaatgttaca tttccaattt
     4681 taattggctt acatagattt tgatattttc tttccatctt ttatatctcc cttcattaaa
     4741 ttttctatat ttaaattttg tattttattt atatatctat ataaaaatgt taatataaaa
     4801 cctattaaag ctataattat agctataatg aaagcccttt gataattgcc atctacagaa
     4861 tatacatttt tcatcactgc aggtccaata tatcctgcaa aagcaaatcc aataaacatg
     4921 attccataat ttacactatt atttttaggt ccaaatttat ctactgtaaa tcctgggaaa
     4981 attcccataa atgaaccaaa acatatacct ataattatta ttccaatata gaatgttgct
     5041 acatctcctt ctcttgagaa taacaaacat aataaaccaa aaattgaaat aactaatgca
     5101 cctgataaag tatttattct accaatttta tctgatatat atcctgcaac aatacgaccg
     5161 aatgtattaa acaaggctaa aattgaaact actattgctg catctgaact tgtcatgcca
     5221 atcatatttg ttgctaaaga agatgctaat gacacacaca ttaatccata gaaagctcca
     5281 caagttaata ttataatcat tagataaaat ataggtgttg aaagcatttc tttccaattt
     5341 ttattaataa taactcttac ttttcccttt ttctctggtg ctacccatcc ttctggtgtg
     5401 aaatctgctg gacatttttt gataaagaaa gaagaaacac atacaattat aagaaatatt
     5461 aaacctattg atttaaatgc agaggttact ccatatttat taattatcca attagtaact
     5521 ggaggcatta ttactgaact aaacccataa gctgctgttg ttataccgcc aactaatcca
     5581 cttttatctg gaaaaaattt aattgaatta cttattgtac atccataaac catgccaacc
     5641 ccaagacctg ttattatccc atatgataaa atcaacatgg gcaatccttt tgaaaaacct
     5701 gccaatatca tacctatacc aaaaattaat cctcctacaa atacaacctt tttaggacca
     5761 aatttatcat ttatcattcc ccctgtaatc atagtaatag gtccaactga gtttgtaact
     5821 gtaaatacaa ttgctaaact agcagctgta aaagttgttc cagcaagact atttaaatat
     5881 tctgccatag gtgatgcaaa tacactccac gcatatattg aaccaataca taaattgatg
     5941 aaacaacttg caattaatat aatccatctt ttttgtgtta aattcattac tctcctcctc
     6001 gataaattta ttaaatagaa tttcttagtt aagattatca caattttttc tatatgtaaa
     6061 tatgaacatg taaatgttta catgttcata tttacataat ttcattttta tatatagttt
     6121 tatttatttt ttgttactca taattaaaaa gttcttatta aaacatagct atattaatta
     6181 attcaaacta aatatatcgt gttataataa gtatgtaatt aaagaaagag gtgtatttat
     6241 gcgaacttta aagtatgcaa ttttaggatt attaaataga aatcctatga ctggttatga
     6301 tatcgtaaaa gagttcaatt ttcaattagc tgagttttgg aatgcaaagc atagtcaaat
     6361 atatcctgaa ctaaaaaagt tggtaaatga aaagcttatt gtatatgata ttaaaattag
     6421 tggtgatgta ttagagaaaa agttatacac aatcacagaa aaaggtaaag atgagttctt
     6481 gaaatggctt gaaaaggatg agccaatgga aagaacccct aaggatattt ttcgtcttag
     6541 aatgtatttt tctaataatt tagatataga cactaggctt attttactag agcatcaatt
     6601 gcttcaacac caagaaagaa ttacgttctt gtacagtcaa atggagcgtt atagtgaagt
     6661 tcctcctctt gatagtaatg attttggtga ttatatagtt cttgatggag ctattatcag
     6721 ggaacaaggt gctataaaat ggctaacaaa ttgtattaac catcataaaa atttggataa
     6781 tactactttg aaataataac ttattgattt taatagtgta tttaattata tacattaaat
     6841 ttataaaaaa gactgtacac aactttgtct ttgtatacag tcttctatag ttgattattc
     6901 aaaatagata attataatat ttaaaacagt taatataaaa gttaaaactt tttattaagc
     6961 tattaatttt atcttcttat tgtccttcaa aattaccttt atatttacaa tctttcacat
     7021 aaaaattact atctcttctc atataaaaat ccagttgatg tcttgttact aaatttaatg
     7081 gtaatcaaat tctttaccaa taagtataaa aattactaat gtgttataat tttcttaact
     7141 actaaatttt acatacattt gaatagaata tatcaataaa ttactataat aaataaaaaa
     7201 ttaagagtta aaataaaaaa tagttaggga gatattatta tgattttata ttttagcggt
     7261 actggtaata gccgatatgt ggctcgtaaa attgcacaag aattaaatga tgaactgata
     7321 tcactaaatc aacttataaa agaagaaaaa acagatgaat taatatccgt agataaacca
     7381 ttcatattcg tatgtcctac ttatgcgtgg agattgccaa tagtggttac ggattttata
     7441 aaaaaaacaa aatttctagg aagcaacaga gtttactttg taatgacttg tggaggagat
     7501 acagcaaaat ccataaacta tattcaaaaa ctatgtaaat ataaggaatg gcaattaaaa
     7561 ggtatggcag aaattaaaat gccagagaat tatatagctt tattttctac aacagataaa
     7621 gaaacatcaa aacaaatgat taaagaagca gataaacaaa tatataggat tatttctgat
     7681 atacgcaatg aaaataaatt tgagactatt actcctagtg gcttaggagg tacaattaaa
     7741 agtggtataa taaatacagt tttttataag acaattatta gtgccaaagg atttcattat
     7801 actgacaaat gcattggctg tggaaaatgt gtagagctat gtcctttaaa taatattaat
     7861 ttaaaaaaca aaaaaccagt ttggaaaaat aactgcactc attgtatggc atgtatttgt
     7921 ggttgcccca ctgaagctat tgagtataaa aataaaactc aaaatagaga aagatactat
     7981 ttagaataat atattcttaa tatttaaacc attataaaat gaactatacc cattgtttta
     8041 tacagtttta catattttaa ctgtctcatg ggtatagttc aaaagatagt gtctcaaagt
     8101 gaacttttta attcctaaat cactcttttt ttattaaatc aagtatactt taatcacctt
     8161 ttcttatatc attttcacta atatgttgta ttccaacaaa tataagattt attagctctg
     8221 ttagtgactt ccattgtgat ttttttgaat caagataata ataatttttc gttccttctc
     8281 tacgcaaatt aacaatctct gcttctttta atatcttaag atgatgagat acagcaggac
     8341 gagaaagatg tgtttttttt gtgatttctc ccactcgtat accattaaaa tcactctcta
     8401 aaagagcaat taaaatcaat tgtctagtct catcacccat agcagagata gctttgcgac
     8461 attctttaaa tttagacgtt atttcttgca ttctttgttg acattctttt tccataaaat
     8521 ccaccctctc ttataagtat ctaacagttg ctacttcttt ctgtgcagta cgtctatatt
     8581 taacatctgg gtatccaatt actaatgttg taagaacatg attactacgt tttagaccta
     8641 aaagcttttt taattttggc gaattatttg ctacatctga aaaatatcca ctaaataaaa
     8701 cacctagacc ataagactct gccattaatg ccatatttga tgctgcaagt gaaccactaa
     8761 tcttatcttt tgtaattatc attattgcaa taggagcatg cttaaagaaa aagttatcgt
     8821 caatctcaac ttcttttgaa tatttaatag ctatatttgc aataggtttt atttttctaa
     8881 aaaatttcac tgcttcgttt tcataaattg actttttatt gtctaaaaca atatatgata
     8941 catcttgtga atttttagca ctaggggtat acctacctgc ctcaataatt tgctttataa
     9001 tttctgaaga aacttcttta tctttaaatt tacgtatact tcttctagat ttaattgcca
     9061 ttagtaattc atctgaatct agctttggct tatttgtaag ttctatagga ggttcatcaa
     9121 atcctgtcaa ggtaatagct tttgtgggac atattgctgc acaatgtcca cacattagac
     9181 aatcttgttt tttaattact gactttttat tttctataat tatattgttt actggacaat
     9241 catttttaca aagcccacat ccaatgcaaa gttctttgtc aacttctatt atatgttggt
     9301 atttcatatt cattcatcta cctttcgttt attagtttaa actattataa cacgtagaca
     9361 ttttttatat tcattggtaa agaatttgat taccattaaa tttttaattt ctctattaaa
     9421 taaatttttc atagaatttt aaaatatcat agtttataca taataaaaaa gacaagctta
     9481 aaacctaagc ttgccttttt catcttttat tacaaagtaa attgcactta cttcccatac
     9541 ttcttaacaa ttttattttc taaaatttta tttgtgaatc atactattgc atcaatacaa
     9601 tgtaaaataa ttctatattg attttctata gttaaaaaag ttaaaaatta tacttattat
     9661 taatttttat actcttctta tttaatacaa agttcttatt taacatagat ttcttgtcta
     9721 atatagattt cttatttaac atagatttct tgtctaatat aaatcttcac catttgaagc
     9781 aattactttc ttataccatt caaaactttt ctttttcatt cttcttagac ttccattacc
     9841 actattgtct ctatctacat atataaatcc ataacgcttt tccatttcac ctgtaccagc
     9901 acttactaaa tcaataggtg cccatgtagt ataacctaat aaatcgaccc catctataga
     9961 aactgcatca tgcatagctt ttatatgttc tcttagataa tcaattctgt aatcatcttc
    10021 tacataacca ttttcatcag ctatatcttt agcacctaat ccattttcta caataaatag
    10081 tggcttttca tatctatcat atacatgatt tagtgtaatt ctcaaaccta atgggtcaat
    10141 tggccatccc cattgactgt atgaaagata tggattttta gcagatgtaa atatgtttcc
    10201 ttcagccata tcatctgcat tttcatcggt tctaacacaa cggctattgt aatatgaaaa
    10261 tgaaataaaa tctactgtat atttattcaa taattcaata tcaccaggtt ccatttctgg
    10321 tgtaatacct ttttgctcca tatactttaa cgcatagtta ggatatttac ctttagattg
    10381 tacatccaca aagaagtaat tttctctatc taactttgta gcttcccata catcattagg
    10441 attacaacta taaggatata cacttccagc tgcaaacata catccaatta agttatttga
    10501 atcaacttca tgtgctattt tagttgcaat tgcactagct acaagctcat gatgggcagc
    10561 ttgatatttt atcttttcaa catcttcacc ttcttcaaag caaataccag cacccataaa
    10621 tggagcatgt aaaatcatat ttatttcatt gaaagttagc cagtacttca ctaatccttt
    10681 atatcttcta aagataacat tgcataattt ctcatagaaa cctatcattt ttcgattacg
    10741 ccatgcacca tactttttaa ctaaatgcat tggacaatca aagtgtgtaa tagtgactaa
    10801 aggctctatt ttatattttt tacactcctt aaatatattt tcataaaatt gaagtccctt
    10861 ttcatttggt gtttcttcat caccttttgg aaatattcga ctccatgcaa tgctcattct
    10921 atatacttta aatcccattt cagcaaataa ctttatatct tctttataat tatgatacat
    10981 atcaatacca gttttagctg gataataatg ctcatcatca aaatcaaaca tttttagttt
    11041 ccctgtaata actgctgttc tatcttttcc tgttggacat acatctacat ttgccaatcc
    11101 tctttcgtct tcattgtatc caccttcaca ttgattggcc gcagtagctc ctccccataa
    11161 aaaatcattc ttaaaactca tagtcaaagc ctcctaaaat tataaccaaa gcactagatt
    11221 atgctaatac ctcaatcttt actaaaataa gactgtaatt agattgtctt ctaattcaat
    11281 atttttactt tctgtttcta ctacatctaa aaattgtgat gaatttgtaa taacaattgg
    11341 agttacagta ctatacccca actcttttat tgcatcaaca tcaaagttta atatagtttg
    11401 accttttgta attctatcac cttgttttat acatgcctca aaacctcttc cctctaattg
    11461 aatagtattt aatccaatat gaataagcac ttcaacacct gtatcagaaa gaatccctat
    11521 agcatgtaga ctaggaaata aggttgtaac taccccatct actggagcaa ctacttttcc
    11581 atcactaggt attattgcca ccccttgacc tagtacacca gatgcaaatg ctgcatcctc
    11641 tatatcactt aattttaata cttcaccttt tataggactt gttactattt ctctttctaa
    11701 tttagtagac ttgacttctt ttatttcttc tttaccctta tttcttactt ttttagactc
    11761 ttctactttc tcatctttat ataaaatcat agttaataca aaagctacga ccattgatat
    11821 aactatacca gaaagagcta ctattaagtt acccattccc ccatctggtt caatcattgc
    11881 tggaagttca aatattccca ttcctccagt tataaatttt ctaagattaa agaatccata
    11941 gaaagcgcct ccaataccac tagcaataca acttattata aatggtttct ttaaaggcaa
    12001 taatattcca taaattgctg gctctgtaac tccaaatata ccagatataa agtttggtat
    12061 tgccatctct tttagttttt tatctttagt cttgaaaaat attgccaaca caactgccga
    12121 acttgcaaat gtacaggcaa agaatggcat cataacatta tcatatccta aagtcataac
    12181 attgtttata tatacaggaa taaatcccca gtgcatacca aaaataacaa gaatttgcca
    12241 agtaaatcct actatagcac ctgccaatag tggactaaaa tctcttattg aaattacaat
    12301 ttgtgaaata agtgtagaac caaatgttgc tatagggcct ataactataa gtgcaacagg
    12361 cactgttatt aataatgtaa gcattggcac aaagaaaaac ttaactaaat ttggaacaca
    12421 cttattaaat aatttttcac acctagaagc aaaatacact ataaatataa ttggtacgac
    12481 tgttccagta taatcaattg atacaattgg tagtccaaag aaatcaatat atactggtga
    12541 tgcaaacatt gttcctttaa ataaagtgta catcacatct gctcctgctg atatactgct
    12601 accttgtata gcaggataac atactgcagc tcctagtgct aatcccaaca ttggttttaa
    12661 tccaaacttc ttagctgaag tatatcctaa gaacaaagga aggaatgtaa ataatgcatc
    12721 tcctgctgaa tttattacca tataaccacc agatacatca ctatataatc ccattgctac
    12781 aaataatgca ttaaatcctt tcaacatacc acaagctgcc attattccta acactggctg
    12841 aaaaattcct gatattatat ctataccttt atctagtata ttacctgatt ttttagtttc
    12901 tgaacttgta cttgaatcat ctaaaccaat tatttcaact acatctttat agacagcttc
    12961 aacatgattt cctataacta cttgatattg tcctccactt ttcattacag taacaatacc
    13021 ttccatgttt tttaatacat tgtcatttgc tatactttca tcttttaaag taaaacgtaa
    13081 tcttgtaata cagtgtacta acccactaat attttctttt ccaccaacat tgtttactat
    13141 ctcttgtgct aattgtttgt attttcccat ttttctcctc catatttcat ttaaattaat
    13201 tttttgaaat ttgaaggttt ggccaactca atgttaccat ccaaccataa gtataatttt
    13261 gttagtatca ctcctttcaa tcattcttaa tatactactg aaaaattatt tcacttacat
    13321 atttgagtct ttaactactt tagctaaatg tattgttaaa tatataagct catcatctgt
    13381 taatatgtag ttatattgtt ttttaatgaa atttttaatt ttaactacac attcaaactc
    13441 tttattatat ttagatttaa taatgcttag taattctttg tctgtctcac cttcatgtgc
    13501 actatctaaa agcaatcttt gcgcaaaaaa ctttaaatgc attataaatc tataataaaa
    13561 cactgattct tcatgaaatt ctatcctaaa atgataccta actatgctaa gtacttcttg
    13621 tattaacttc gtaatatcag atgccaaagt atgaccttca cttaactgag cattcactat
    13681 atgaaaggct ataaatccag cctcatcttc tggtaatttt atacctgttt tgttttcaat
    13741 aatatctagt gctttcattc caatttcaaa ctcttcttta taaaatcttt taatttccca
    13801 aaggattgca ttttttacat ttattccttc tttcatcctt tgaattgcag aatatgtatg
    13861 gtcacttaag gatatgtaaa tactttcatt tagcttcttg tttaacttta tttgtgcgta
    13921 ttctataatt tcacttgata atttcatgtg ttctatagga atattattga gtaattcttg
    13981 tagtttgttt gatatttctt tatttgatat tctaaatact ttatttactt tagttttatc
    14041 tattagattg ccttttgatt tttgataacc aattccttta cccataacga taacttcttc
    14101 atcattatca tctaatgtga ttacaacatt attgtttaat atcttattaa ttatcatgat
    14161 tatcctcctt ttaagcataa aaaaagctta tccataataa tgggcatact ccttcctagg
    14221 atactgctat tattaatgaa taagcttagc ttgtacttaa acaattacta cctaattcca
    14281 attaatatga taaaacatca gacatatttt gtcaactttt tatttccata tacttcatca
    14341 atcttttgtg ttgctttttg tcagttataa taattattct tattaatatt aatttaatag
    14401 agtccatttt taatagtttt attataattt ttaaaatcaa caacattaaa tcctttattt
    14461 attagaagat tatatgttgt tatatgtttt aaattatatt ttctttctca cctctaaact
    14521 attctctatc tcttatttat ttctgtaata aaaaacgacc ttctatagta atttaagctc
    14581 tcacaatagg ttgtttttcg atttataaat tcaattatta acctcttcat ttgacttaaa
    14641 atctatacta gatttgtctc aataaaaaat ttataagtct aatttaatat ttctttgagc
    14701 caatataaat ataaaacaat ttatagacaa tactactaaa aattctatta aaatcaataa
    14761 ataattaata ttataagctc tctgcaacaa tgaaattagt aagaatctaa atagttcata
    14821 aaaaattgct ataagaatac tttggataat tgatacaatt atcatttcta ataaacctat
    14881 ttttatcaat tttcgtttac tcattcctac tctatacata atttgataat ctagtactct
    14941 ccttataaaa ataattattt gagatgttag tattagaagt atacttgaca atgacacagc
    15001 aaatataata ttcaaaagta atgacttttg atcatcaaca tatgttttca tagaataagc
    15061 aaaatcatta tagtcttcta aattagcata tttatcatta tgtaataatt tactaagtaa
    15121 agtgttatct aacttttctt taaaaaaata tattactggt tgatttttag ataaatcaag
    15181 ggttttaggt gaaatatttt catttatatt tatatatcct aaatctacac ctttattcaa
    15241 tgattttgga cttaaaaatc ctattacttt aaagtctatc ttctttccat taattcttaa
    15301 ctttacagta tcgcctagtt tataatctat taagtatcta tgaagataat tatttataat
    15361 tatactattt tctttatcat tttctttgaa ccagtttcca tactctaaat cactttttaa
    15421 tattgtctgt ttagtattga ataagtcaac acttaagtca tttaaatcat tcattacatc
    15481 gtttctattc tcatatggaa ttttttcaat ataatcatta acagaaatat tatttactga
    15541 ctctatacta tctattttaa ttggataata ttggaaataa tctatatcat tttcgataag
    15601 ttttttatct gtaattaatt ttccttctaa agttgtttga tatacaattg aattttttat
    15661 actattatca acttcataat tgctaaacat agcaaaggtt atttcagata tcataaacaa
    15721 caacaaaaca aatattattg ttattgttaa tagactagag ctaaaagcat tggttttaca
    15781 atcattatta agatatataa aagtaatcga gtattttccc ttaataattt ttttaattaa
    15841 acataccatt tttaataatg catatatcaa actgtatacc aataaatcta gtagtattaa
    15901 tcctaaaata aaaaaaatac ctagaaagga cttcacatat attgtaaaag aaatacttgt
    15961 taaaaatatt acaagaatta tatctggaat aaagttaaat ttatatgaag acaacatact
    16021 attatctcta tttattcctt cataaggtat ttttttaaca aaacttatag gaattaatga
    16081 gaatattgat gtttcaatta ttataataac tattgaaatg aaaatatttt tgataatact
    16141 caaaatagaa atattaaata gattaatatg ttgtaagtta atatagaata tagttatagg
    16201 aatccccaaa ataataccta ttataatccc ctttatagta attgacataa gttcagtaaa
    16261 tactaaaaaa gataaacttc tttttttaat tcctaaagcc ttaaaagttg aaaaatcatg
    16321 tattcgattt aaaataaata caataaatgt agaacataga caaattccag tgattaataa
    16381 tgaaatagct ccaattaaat ttattgaatc taattgcata gatattctct ttattattga
    16441 ctgttctagt tcatcagttg ttctaacttc aaaatggttt ttaaatacat tttttatttc
    16501 atttttaata tttttcacat tttctttgtt tattatattt atataagcta cattatattt
    16561 agtttcatta ggcaaatata cataactttc tccaactacc tcttggccaa cctctgcttc
    16621 tttttgagct gtacttaatg aaaacttttt tggtattata tcactaactt taaaatatgt
    16681 atcatcataa actgaagaat gtagttttaa aaacacttta tctcctatat ttacatttaa
    16741 tttattagct gttttttggg ataaaactat atctttttca tttcttaatt ttaaatatgt
    16801 attttgcatt tttatattat ttactacagt caattcagta ctttcaatca taccatttga
    16861 caatatattt gtattcatac tacttcccaa ctgaaaatca atagcttctt tatcttttaa
    16921 ttcttctaac ttatctattt gttcacttgt aaagtagtat ttattatatt ctatattttg
    16981 tatttttata tctccaccat ttgttatagc catatttctc ttaacttcac ttgaatatgt
    17041 atccgttaac actcttgtaa aaagtaatat tgctgtggca aatacaatag atattataca
    17101 agagattttt aataatttat tattcttcaa actattctta ctatatattt tataaacttc
    17161 atgaaatatc aatgtatcac cacctatttt ataatagttc catctttaat acttataatc
    17221 ctatcactat attctgccat ttgaatatca tgagtaacca ttactattgt agtaccatat
    17281 tcattgttta tattcttaaa caatttcatt atagcatgac tattagtact atctaatgat
    17341 ccagttggtt catcagcaaa gagtatattt ggagaacaaa ctagagctct tgaaatagca
    17401 actctttgct tctcaccacc tgataattgt ttaatattat tttttgtttt tgatgtcaac
    17461 ccaacctttt taatcatctt atctactatt ttataatctt tattatcaga aaaaatcaat
    17521 ggcaatttta tattttcttc aacagacatt gatgtaatta agttaaatga ttgaaataca
    17581 atccctatct ttctatttct atattcagca agttcatcat cagttaattt cgataaattc
    17641 tcaccacaaa ctttcacgtt tccagtataa tttttatcaa gaccacttaa tatacttatt
    17701 agagagcttt tcccactacc actagcaccc attatagaaa caaattctcc ttcttctatg
    17761 tataaatcta tattccttag tatagtaagt tctgtgttta acaagtctac aactttagat
    17821 aagtttgaaa cttcaattat catttttttc ataccattat ttcctttcat acaaaacttt
    17881 taagattgat tattaattaa aacagagtat ataggacaca aaatattaga gaaacttata
    17941 caagcaagct tttttgagaa tgatatattt tctaaaactg tacaaatctt aataggtcat
    18001 tatatttctt attatacata agattgcttg accaaaagtt agtaaaatta taaaaaataa
    18061 actttttcaa tgataaatct aaaaaatata atatcatcaa taattcggaa gtgggattta
    18121 atttaatact aagttttagc ttagatatac agaatctctc cccaaaattt atatatttat
    18181 aacactttat gttataaata tatgactctt tcaaataaat ttcaacagaa tattgcgaat
    18241 acttgatatc atcttataag tataatttat aaaaaatttc atgataaata tttaagaatt
    18301 acagatacta ttataaaact acttttttat tacattttat agaactctcc acataaaaaa
    18361 tgcttataac ttatgacata aaatgattct ttaataaaca ttacaataca aggctaaact
    18421 attctattaa tcatattatt tattaaccac catcctaatg gactaataac ttttacatgt
    18481 acaattttat acattataaa tcctatagta cacccaagag accatcttcc tgtagtagga
    18541 atataggtat caaatttctt ctccaaaata aaaaatatgc caaaccttaa caaaacatat
    18601 ttttatatat tttactaaag tttgactatt taagaatcgt attttttaaa gttttaaatt
    18661 gagcttatct taattattat ataactatta tttactaagt tttataggtt aatcactttt
    18721 attataaata tttatcatga atttttttat ctcatactat ttaacacact tccttaattt
    18781 acctatacct tccttaacat ttttttcaac tccaaaaaca tatgtcataa taaatggagc
    18841 aaccaatgct ataaatataa ctataagtcc atttactata tttgaaatcc ccttttcccc
    18901 tgataatcca agtactgcca ttatactatt ttgtggaaca tataaagtca catgagtaat
    18961 tcctgcaaat aaccctgcga ttcctgctcc tatagatgct aacatagcag gttttttaag
    19021 tttcaatgta actccataaa gagctggttc agttataccc atgatagctg atatccatgc
    19081 agctgatgct aactgtttca tcttactatc tttgcttctt aatgctacag ctaatgtcgc
    19141 gccaccttgc gctaaattag cacaaacttg agatattaat attagcgatt cttgtcctgt
    19201 agttgcaaat gctgatatga atataggagt caatgcatga tgcatccctg tcataacgat
    19261 aaatggcatt gcagctgcta ataaacaaac cattataaat cctaaccttc cttgtagtaa
    19321 atttattata ctagctaatc cctctcctaa gaaatttcct attggtccaa ttactattaa
    19381 tgctattggc gcacttatta ataatactaa agttggattt aaaattgttt ttaacatgct
    19441 aggtgttaat ttatcaatga atttttctat atatgacatc atccatactg ttaaaagtgc
    19501 agggataaca gatgctgcat atataacacc ttgaattggt actcctataa atgatatagg
    19561 gtccccactt gatacccatg cagagaaatt tggatgtaaa aacacaccta ctactataac
    19621 tgctattgat gtattaacat taaatttttt agctgcattt attgcaagta ttataggcat
    19681 aaagtaaaat gctacatctc ctattatatc taatactcta tatgtacttg aatttgatga
    19741 tagccaatta aaagtggtac aaagagataa tataactttt atcatcccag atgctgtaag
    19801 ggctggtatc ataggtgtca tacaaccagt tattgtatct attatattat ttaaaatact
    19861 atttttttta tttttatttt ttatatcgct tttaatatct cctgtggtat ttatcccctt
    19921 atttattatt gcattaaata caacacctac ctcattacct attatgacct gatattgagt
    19981 tgaagttgag tttactccaa ctaccttatc tatagatttt aatttatctg cattgacttt
    20041 cttttcatct tttaagatga accttaatcg tgtcatacaa tgagttaagt caattatatt
    20101 atcttttcct ccaagtccat caattatcaa ttgagctaca tcttcatatt tattcttcat
    20161 accttatcct ctcttttaaa ataattttta atatagtttt aagccaaatt aatcttttta
    20221 ctccaagctt gtaatagtgt attgtaattt aataagctag tatcttctac aatatggtca
    20281 cattttccta gtacttcttt tttccccaca ccaattgatg tcatattggc agaatttata
    20341 gctttaatac ccgaataaga atcttctatt cctacacaat catattgttt taaatttaga
    20401 atcttgctcc cttcaataaa aatatcagga tatggttttc ccctttctaa taaggatgga
    20461 tttactatac aatcaaattc atcttttatt cccaaagatt ctaatatcct aggtgcattc
    20521 ttactagcag atgctattcc caatttcaca ttattttttt taagttcttt tataagctca
    20581 tttatccctg gtaatatatc tttatatgtt aatgagttta ttaactttat ataaatgtta
    20641 tttttttctt cacacacctt taacttttcc tctttagtat aactatctac tttattagca
    20701 gataacaata ttttatctaa tgattcttcc ctgcttatgc cttttaattt ttcattaaaa
    20761 gtttcatcta tttctatatc tagcgatttt gctatctcat gccaagcttc aaaatgatac
    20821 ttagcagtat ctgttataac tccatcaaga tcaaataaga ctgctttcat attaattact
    20881 ccctttaaaa caattattaa cttctattat gttctttgat aattttattt ctttattatc
    20941 atgtaatatg gttatatttc tacctttgat taacttataa taagatttat attcttctat
    21001 atttacagaa actaaattat ctctaaataa tatattaaat ttaatatttt tccagtgttt
    21061 aggtatcctt ggttcaaaac ttaatttatc attttttatt ttcattcctg caaatccata
    21121 tactaaactc atccatgttc cacccatatt agcagcatgt attccatcac ttgtattttt
    21181 ttgtaggtta ttaatatcca ttaatgctgt gtccataaaa tagttataag ctttttcatg
    21241 atttccaatt tcacttgcca tcattccaaa tattgacctt gacaaagatg aatcatgagt
    21301 agttattttt tcatagtatt caaaatctct cttcttttgt tctaagctaa actcatcagg
    21361 gaaaagcaac gctgaaagta cagtatctgc ttgtttattt acttgatatc tatatattgt
    21421 taatggatga taatgaagaa gtaatggata tttttctttt ggtgtatttt taaaatccca
    21481 tactgatttt tctaaaaatg aatcgtcttg tttgcttatt ttcttctctt catcatatgg
    21541 taaatacata ttttgagcgg catttatcca gtttttcact tcatattctt gtaatttaac
    21601 ttttctaaat acttcatttg aaaataaatt atgcttaaat ttatctacca tctgtactgc
    21661 atatattaaa ttatatttag ccattaaatt agtgtagtaa ttattgttta ctattgctgt
    21721 atattcatct ggtcctgtta ctccatttat acagaattta ttattttttg attctatata
    21781 atctccaaat tcaacccaaa atcttgctgt ttctataaga atttctaagc cattttgcat
    21841 cataaattct tcatcattag ttgcttcaaa atacgttcta ataccataag ctatatctgc
    21901 atttatatga aactgtgctg ttcctgctgg ataatatgca ctgcattcat taccatttat
    21961 agttctccat gaatataagc aacctttatt tattccaagt tctctagctc tttctcttga
    22021 tgcatctaag attgagtatc tataatctaa taaagctcta gcaatggatg gttgagtata
    22081 tataaaaaat ggtaatatat acatttctgt atcccagaaa taatgcccct catagccatc
    22141 acctgtaaga ccttttgctg atatattagt ctttccatct cttcctgctg attgataaat
    22201 atgaaataaa ttggttttta tccccagttg aagagtttta tcgccttcaa tctctacatc
    22261 agaggttgtc caaaactcat tcattttctt tctctgaata tttttgactt tatcaaatcc
    22321 aatattacat gcttctctta aaaccaattc taatgatttt aaatgtgctt ctgattctaa
    22381 acattttaac gactccagac attctaatga ttttaaacgt tcttcattta gatttaaatc
    22441 agaatatact ctactatagc ctacaacaat ttctaaattt aacctttcat cttttttggc
    22501 atagacttct atttctcgct caatacattc atctgaaaca ttataggtct catttgtaat
    22561 atttccacaa gtaatatatg aatcaaaccc acaccataac cctaatctac ttctttctgt
    22621 aactaaattc ataaaatact tattgttata tttttttaca ttaattttaa attgcttctt
    22681 atttatagat gccactcttg ggtcatctac tttttcacaa ttaaacttct tcatactatt
    22741 ttccatttta tttttaaatt ttatttttcc attaaaattc aatggtgtta ttgtatatga
    22801 aatcatcatt aattcttcta tatcttgtga tactaatctt tctatagata ctttaacttt
    22861 tctaccacga gagttttccc atataaatga acgtttaaga atcccttcat ttaaatctag
    22921 tatcctagaa tgttcactta taataccttt tgataaatca aaataatctc cttcaataga
    22981 gaaatttact atatgagcat taggtaactt acagatagtc tgattttgtt ttgcatagcc
    23041 ataagcgctt tctccatatg taattggaga ttcttcataa aatccattta catatgtacc
    23101 catattatct acatactcat tattaaaatc agactcttca atattccctc taactcctaa
    23161 atgtccattc gctagtgtaa acagcgtttc attggatttt attgtatcct tactaaaccc
    23221 ttgctctata agttggtcct tagtagtaaa tattaaactc ataataacac tcctttctta
    23281 aaatgaatta tgcatgagtt tccatatcat taaataaaat ttctccatct gtttctatta
    23341 cttttttata ccaaaaagca ctttgttttt tagttctttt aaatgagcct cttccatagt
    23401 catcagaatc aacatgaatt atgccatatc gttttgtcat ctttccttca cttacactgg
    23461 ttacatctat acatccccac ataagatatc ctataagctc tattccatct tcatttactc
    23521 caagaatcat atttttaata tgttctttca tgtaatctat acgataatca tcaattattt
    23581 ttccatcttc atttacaata tcttgagtac ccagtccatt ttctactata aataaaggta
    23641 actgatacct ttcatataac tgattcatag ttattcttaa ccctacaggg tcgttttgcc
    23701 atacactatt tgattttgga agatatggat tttttatggt ttctggtccg tttgcctttg
    23761 atttttctaa accttcagct ctagctgaac tacaagcact agaataatag ctaaaagata
    23821 caaaatctac agtattatcc ttaagtgttg ataaatcttc ctcttttatt attaatttta
    23881 tttgatttga ttttaaataa taattcataa agcttggata cacaccccta acttgaacat
    23941 cagaaaaaaa caaattatgc ctttcatgct ctaaagttgc aaatacatct tctggtttac
    24001 atgtgtatgg atatgttttt ccagcagcta acatacaccc aaacttaaat tctttattta
    24061 tataccttcc tatttctata gtttttgcat tagctaataa ttgatggtgt gctgcttgat
    24121 attttttctg aagttgattt tcatcagcta taaaagttaa cccagcacct atatatggaa
    24181 tatgcattat catattcatt tcgttaaaag gaatccagta tttgacatca tctttatatc
    24241 ttttcatcaa aatttcagca tattttacat ataaattaac cactcttcta tcagcccacc
    24301 cagaatattt ttcaactaga gaataaggta tatcaaagtg gcttatcgtt attaatggct
    24361 ctatattata tttttttaat tcttttatta tattatcata aagttgtaat cccttttcat
    24421 ttggttcact ttcttctcct gttgggaata ttcttgacca agatatagaa aatctaaaac
    24481 atttaaaacc tatttctgct aacattgcta tatcttcttt gtaattttca caaaatttaa
    24541 tccctatatg acttggatag tattgattat tttcatttaa aatattatcc tcatctaaac
    24601 atacatcttt taatctctta tcatttaatg gtagtttatc aaactgagat aaaccttttc
    24661 ctccatctaa ataaaatcct tcactttgat tggctgaaat tgcccctccc cataaaaaat
    24721 ctttccctaa tttcatgcat ctacctcttt cacagtataa agttttataa tttctatctc
    24781 atatttagtt ataagatttt tttcatacta taccttttaa ataattcaat ttaaaaatcc
    24841 aattatattt tttactcact ataattaata ttgtctattt tcaatacttt catattaaat
    24901 atcaatatta gatatctatg tatataatta tatatatagc aaatacattt ttatatcctc
    24961 attattgtta tcttattctc atatattgct gtatatttat atatataaaa ggagttattt
    25021 cgaaatagag gttgtattga gaactaaaat ttagacttta taaatgaatg gaattttcat
    25081 tgttgaaata ctgaaaatat atttgatttc atataaaaga aagtgcctca tgaactaagt
    25141 tcattttgag acactccctt ttttttccaa gctattatat ctttttttaa attctaatgg
    25201 agagactcca attttttctt taaatactct gtaaaaagtt tttgtacttc caaaaccaac
    25261 ttcttcaacc aaatctacaa tggatatatt gttattaagt aatatccact ttgctttttc
    25321 taatctatat tcatttaaaa aagttataaa tgtttttcct gtattttttt tgaaaaattt
    25381 agtaaaataa tatacactaa atcctacttc ataagacaca tcttctaatt ttataggttg
    25441 tttataattt ttttctacat atttgaatat attgttcaat ctctctaata tctctttaga
    25501 gtttatttca tattctactg tattttcttc aatttctcta ggaatctgtc tatataatat
    25561 aggaattaaa ttatacattt taccttttat tgcataacta tatccttcta ttttgtatag
    25621 gtcttcattg tagatatcta tcaatatatc gaaaactgac tttcttattt ttgctgacca
    25681 atctctacta cattttttta tactactgaa taaattaatt aagctttctt tctcctcatt
    25741 taataaaatt aaatcattaa aaaaagatat atcaaattga aatactaatc tttcacttcc
    25801 cggtgatggc agtatataat gaatatctcc tccttttatt atcactatct cccctttttt
    25861 taattgcata ggcttgtcat ttataaatag atttactaca ccttctgtta taagtaatat
    25921 ttctatttcc ttatgccagt gtggtgttgt taatacttct ccttcatttg atagactacg
    25981 aaaaggaaat tcatgtatta atttaggcat ttctaaatat attgccatat atatcctctc
    26041 ctctctagtg tctaaaaatt aatttatact atttaaaaga atacacattt tattttaaaa
    26101 acctcaaatt tataatttat acaataccta cacttcataa aatatatcta atatttcttt
    26161 ttttgtatgt gcatttaaaa gtctttcacc tatattttta ccacctaaaa tgtaagctat
    26221 tttagatatt aatatcatat cttcattgcc tccagattgt ggcatagcaa acataattat
    26281 tacctttaca ggtaagccat ctatacttgg ccaatctaca ccttttttag atttacctat
    26341 tactatggat gccttcttta catattttgt ttttccatgt ggcatggcca tttgataacc
    26401 tatactagta gagcctattt tttctctatg ttttaatgcc tttataaact tactttttaa
    26461 agttatactt cctgtatcgt ataatttatc tactaattcc tgtattatgc cttcttttgt
    26521 agttgatttc aaatctaaaa taatgctttc ttctgttaat atttctctaa tttccatttg
    26581 ttttctccaa tagattattc ttaaataata ttttaatatc ctctttgctc ttgcaatcta
    26641 ttatatagtt tatatagttc ttttgcctat ataaatttga tatttccatt aaaatattct
    26701 ttaaaaaact ttgattacta atttctaaat aaatatcaat cgttctagta acattattga
    26761 tattcattat ccctttattt aatttacttt gcttatgtat atatatattt aaatcttttc
    26821 ctaatgaaat agattcatat ttactcccta aattcacacc tattaaactt tccatctgag
    26881 ctacatcaaa attaaaatat atattatctt ctaaatacat tcctaataag tttgtatcta
    26941 agttcatact ttttttaggt ttagaaagta aagcattttt tatattatta atatcatttt
    27001 ccattaatag tactgataca actatatgta atttatcttt caaatctaag tttacagtag
    27061 atattacaaa gtctacatca tctaaatttc gcttactcaa ctgatgcata ggaactatat
    27121 caactatagt ccattcagga aattctcttt ttaatctggc tgcaaggagc tgagatgttc
    27181 catatcctgt atgacaaact actattactc ttttactact catatttctt tctattgccg
    27241 cttgaaaata tgtggcaaga tatgaaactt catctatact aatattcttc agattatcat
    27301 actctgtcat aatatttata gtcatcatac ataatcccag cacatctgaa aatcgctctt
    27361 gtatctctcc taacaacggg ttttttattt ggatatcata tcttaatctg tttaacatag
    27421 gccttacatg agatgctaga cttttctgta gtaagtcatc atttttgaga tttatattta
    27481 aaaatcgtga catattctct atcattatag atgaaacttt ttcactttca cttaatctat
    27541 ctgattcatc attgtaacta ctattatttt cgctactaaa tccagatgat accaaaaaaa
    27601 tatatatgta cataagttca tctttcgtta tttttactct gtatctattt tctattttat
    27661 ctataagtgt agtgacattt ttatatacta actcgtccaa attatctaca gaaatttctc
    27721 tttctttctg agattctatt tgattacctt cttcgattct ctttaaacat ataagtatat
    27781 gggtaattaa atttatatag tagggctgat ttatagtata tcctaaattg tattctaatt
    27841 ctgtaattat agtttctaca aatataatag aatcaatatc aaataaattt atcaatgcac
    27901 taaatgtagc agaatctaat cttgttaact cagatatatc atgttgttca taattttcat
    27961 ctaataaatc atccatcatt ttggcaatcg atttccttat atccacttct ttacctataa
    28021 tcttagttcc ttctaaagtt ttattaagtt ttaaattgta ttcttttatc catgcctcta
    28081 tatatttaaa atcatttact atagaagttt tacttacata atatttatct gctaactgat
    28141 ttattgaagt tatattattt gaatttagca ataaatactt taaaatctcc attcttctct
    28201 gctctactga taaagattta ttttccttcg attgtatatt taaactatta attaagtcta
    28261 tgttcttctt tgctaaatct tctattaata ttccatagcc tctttttata tcaattttta
    28321 tattaaaagt ttctaaatat ttctcaatca tcttcaaatc tttttgtaaa gttttatcag
    28381 atacatttaa tttatcttta aagtatttta taggtctata tttattttcg tttagtatca
    28441 atttaagaaa ttcaatttgc ctcgacacaa gcatacttag ccctccctac ctatgtttat
    28501 atatttctat gtttatatta agtactattt taacatattt actaactatg taaaattact
    28561 aagatacttc tgtatcataa aagaatttat gatacacctt actaaattga tataatagat
    28621 aatataattt caaaataaaa attaaggata tttagaagta gttaactgat tctaaatatc
    28681 cttattatta ttctatatac ttgttgttat tccattaatt atttttacta ttatttataa
    28741 tttaagcacc aagtaattca acttcactat tttcactcct tatagtaggc tttgttaccc
    28801 ctataatcaa tgctgttatt actgtaccaa tcaatataca agctatatat gcaactggtg
    28861 cactacatgc aaatggtatt acaaatattc ccccatgtgg tgccattaaa gttatatcta
    28921 aactcataca tattgcccca cctatacaag aaccaattat atttgctgga atcattctta
    28981 atgggtctgc agctgcaaac ggtattgcgc cttctgttat gaaagatgcc cccattatag
    29041 cagttggtag agatgacttt ctttcttctt tagtaaactt atttttgaat attaacatag
    29101 ctaatgatat tgctaatgga ggtaccattc ctcctaccat taatgctgct gatggttcaa
    29161 atatattact tgaaaacata gccaatccaa aagctgaagc tgttttatta ataggtcctc
    29221 ccatatctga tgccatcatc cctgctaata ttgcacctaa taatacttta ttagttccag
    29281 acatactttg taaccaagat tgtaaagctt catttaacca tgatgctggc gtattaacta
    29341 caaagaacat tatagctcca ctcgtaaatg caatcaaaag tggacatatc aatacagcat
    29401 ttactccttt taatacatct ggtaaatttt ctgttatctt cattactcct aaagctatat
    29461 atcctgttgc aaaaccagct accataccac ctataaaacc tgaacctcca ataactgcca
    29521 taaaaccagc tatcatagca ggagcaaatc cttgtcttcc tgctattgat ttagctataa
    29581 aaccagcaag tatcggtata aataacttaa atgctgaatc gcctccaagt tgtccaaaga
    29641 atccagctat agggttgtat gtttcatgag ttgggtctga tgcaaatata ccaaacataa
    29701 aagctatggc tataccaatt ccacctatca ctacaaatgg aatcatttca gatacaccac
    29761 tcattaagtg cttataaaca ccctttttct ctgacccttg aagcatttct tttgaatctt
    29821 ctaaagtctt tccacctggt ttaatttctt tagccattcc attttcagcg tttataatta
    29881 aatttttagc taatgaaaca ttcttagcat ctactacttt cttttgtagc actttctttc
    29941 ctataaatct actcagttga acttctttat cacaagctat tattacgtat tcagctcttt
    30001 ttatatcttc tttagtcaac ttattttcta cacctatagc tccatgagtt tctattttaa
    30061 cttcatatcc aagttcttgc cctgcttcct ctaatgcttt ttttgccata aaagtatgag
    30121 ctatacctgt tggacatcct gttatcccaa ctataagacc tttattttga ttattactaa
    30181 aatctcctga ttctgcagct ttattattaa atatacttaa tatttcgtat ggagtttctg
    30241 cacttagtag atgcttaaga aaattttctt ccattagata tgtagctagt ttagatataa
    30301 tcttaagatg ttcatctgaa cctgattctg gaactgctat cataaaaaat gacctaagtt
    30361 tttgaccatc attatctgta atctctctat ctgttcttaa atacatcatt acaggttgat
    30421 ttactacatt tgacttagca tgaggtatag ctattaaatt acccatatta gttgatgatt
    30481 ctttttcacg tttattaata tcagctataa acttttcttt attatttata taatttccat
    30541 ggtacaaatg atttgccata taattaatta catctttttt tgtttgtaat tgtttatcta
    30601 gtacaataca atcgcttcgt agtattttac ctatcataaa ttctcccctc cttttaattt
    30661 gttacctcat aattaatttt tatttttata aaagcacttt aacttatcta tagcattgaa
    30721 tagattcatt ttaaactcta taacttctct tgcagctttt atacatggtg gatatatagt
    30781 atctggctca tataagcttg ggttttgttc taatagttct ctgcacttct tataatatgc
    30841 cgacttaaca tcactcgaaa tatttatttt acaaactcct agtttaactg cttgtgctat
    30901 ttcttcatct ggatttgatg aaccaccatg aagaactaaa ggtatatcta ctacttctct
    30961 tatttctttt aataaatcta gtttaagctc tggtttaaaa ccttttggat atataccatg
    31021 tgctgtacca attgcaatag ctagtgtttc tactcctgtt ttttcaacaa aatccttagc
    31081 taattttgaa tctgtatata ttatttcatc agtaccacct tcacttgatg ttcctgttgt
    31141 tcctattgtt ccaagttctc cttctacaga cacatttaat ccttttgcta tttctactat
    31201 ttccctagat atagctacat tctcttcata tggtaaatgt gagccatcaa tcattactga
    31261 tgtaaatcca catcttattg cctttaatat ttgttcttta ttttccccat gatctaagtg
    31321 tattaccata ggtactttac tcttatttgc ttcttctatg caactagcta caaagctatc
    31381 tcctagaaaa cttagctcat ttgggtgtat tgcaagtatt actggtgcat ttttttcgtt
    31441 agcactctgt actactgctt taagtatttg cccacttcct atattaaatg ctggtacagc
    31501 aaattgattt tcttgtgcca cttttagcat ttccttcata tttattaaca tattgtacct
    31561 cccgatattt taaattttag gttgaatttg cttcttgtta attttgttgt tgtttatatt
    31621 taaataatat cagggtaatg aagctcagaa aataacaatc gttttcctta gtgaaaagga
    31681 acttttatta tacactctaa gttccttttc actaaataat ttttagaagt ctgcatcata
    31741 gttaattata tttctcatgt acatcttctc tttttaagtg ttttatgctt tagccttcct
    31801 agattttata ataaacaatt aagatacatt aactataaat ttaaagatac tcatgcaata
    31861 gttaatgcta tattagagtc aaaaaaatta aaaagatatt agctatttgg ccaatacctt
    31921 ttcttggatt ccaatagtat atacgattta tagaaatctt actcacgatt atctaatgtt
    31981 ttacttccat aaactttgca taaaacacca aagtgacacc ctccaaattc atctctagct
    32041 aataaagtga tttggttttc taagcaccaa tttataaatt agcctttgtt atcttatgta
    32101 aatctttttc tatctaattt aaaaattatt cctaatgcta tatagcatat accaattact
    32161 aatacaccat aagccataga tactcctatc ttaaattctt gcatattaat tacaatagaa
    32221 actgaaatag gtttattaca aacaccataa agcaaggctg acattgtata ttatccaatt
    32281 gtatgaatga atactaatat agctcctttt aattaaatga tgctatataa atcatattta
    32341 tcccaatttt taatctaacc tatttatatg tatattcctt tttaataaaa aaagatattg
    32401 actaaatagt caatatcttt tagttgattt aaatacccac aattttaatc taatttttaa
    32461 gtgtttcctt aacataatct tataaatatt aaaatttatc tacctataaa atttctccat
    32521 taaattttat tacattttat cttaaaacaa ttataatctg accttaatca catgttatat
    32581 tatataaata tttttaacaa acttaatgat taaataacta tattttatta tccatcttat
    32641 aaatgtttat aaattttcca ctattcttct tcaataaatt caaacaaatt ttttactgta
    32701 gtatcgaata ctttagcgat atccatagct aacttcaaag aaggattata tttcccattt
    32761 tctaagtgta caatagtctc acgtcttacc ccaaccaatt tagcaagttc attttgcgtc
    32821 ataaggagtt tttctctgta ttctttaatt tttgttttca tcaatattgc cacttagtta
    32881 catatccccc ttttatcaaa gtatatatac aacactaatt tagataaaga ctgtataaat
    32941 aaaactgcaa tgaggattat acctgtatct aggtttgata tatttatatt acttgtataa
    33001 ggtgttgcaa agaaaataga tattattact atagaatata tcattatttt tattgcaatc
    33061 ttattaacct ttgatagtat aaattcagat gattc
//
LOCUS       DAEORZ010000033        31296 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEORZ010000033 DAEORZ010000000
VERSION     DAEORZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.033"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            218..763
                     /locus_tag="KRH39_001407"
     CDS             218..763
                     /locus_tag="KRH39_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBG8511324.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            795..1931
                     /locus_tag="KRH39_001408"
     CDS             795..1931
                     /locus_tag="KRH39_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBG8511325.1"
                     /translation="MEEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            1935..3326
                     /locus_tag="KRH39_001409"
     CDS             1935..3326
                     /locus_tag="KRH39_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG8511326.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLRVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            3402..4277
                     /gene="dpsA"
                     /locus_tag="KRH39_001410"
     CDS             3402..4277
                     /gene="dpsA"
                     /locus_tag="KRH39_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBG8511327.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCIVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINAILCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            4280..4870
                     /locus_tag="KRH39_001411"
     CDS             4280..4870
                     /locus_tag="KRH39_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBG8511328.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(5077..5277)
                     /locus_tag="KRH39_001412"
     CDS             complement(5077..5277)
                     /locus_tag="KRH39_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511329.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            5781..8435
                     /gene="adhE"
                     /locus_tag="KRH39_001413"
                     /gene_synonym="adhC"
     CDS             5781..8435
                     /gene="adhE"
                     /locus_tag="KRH39_001413"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG8511330.1"
                     /translation="MAHKEQVIIENKKQNEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            8711..10930
                     /locus_tag="KRH39_001414"
     CDS             8711..10930
                     /locus_tag="KRH39_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8511331.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSFVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            11184..11846
                     /locus_tag="KRH39_001415"
     CDS             11184..11846
                     /locus_tag="KRH39_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBG8511332.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            12217..13626
                     /locus_tag="KRH39_001416"
     CDS             12217..13626
                     /locus_tag="KRH39_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBG8511333.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLASSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            13741..14016
                     /locus_tag="KRH39_001417"
     CDS             13741..14016
                     /locus_tag="KRH39_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBG8511334.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            14646..14957
                     /locus_tag="KRH39_001418"
     CDS             14646..14957
                     /locus_tag="KRH39_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511335.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            14959..16884
                     /locus_tag="KRH39_001419"
     CDS             14959..16884
                     /locus_tag="KRH39_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBG8511336.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            16887..17378
                     /locus_tag="KRH39_001420"
     CDS             16887..17378
                     /locus_tag="KRH39_001420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBG8511337.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            17430..17993
                     /locus_tag="KRH39_001421"
     CDS             17430..17993
                     /locus_tag="KRH39_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBG8511338.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            18008..18985
                     /locus_tag="KRH39_001422"
     CDS             18008..18985
                     /locus_tag="KRH39_001422"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBG8511339.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            18972..19295
                     /locus_tag="KRH39_001423"
     CDS             18972..19295
                     /locus_tag="KRH39_001423"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBG8511340.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            19412..21190
                     /locus_tag="KRH39_001424"
     CDS             19412..21190
                     /locus_tag="KRH39_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBG8511341.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            21187..22560
                     /locus_tag="KRH39_001425"
     CDS             21187..22560
                     /locus_tag="KRH39_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBG8511342.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            22566..23234
                     /locus_tag="KRH39_001426"
     CDS             22566..23234
                     /locus_tag="KRH39_001426"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBG8511343.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            23512..24561
                     /locus_tag="KRH39_001427"
     CDS             23512..24561
                     /locus_tag="KRH39_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG8511344.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            24657..25646
                     /locus_tag="KRH39_001428"
     CDS             24657..25646
                     /locus_tag="KRH39_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8511345.1"
                     /translation="MSYLKINNVFKSYDQKKVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            25651..27369
                     /locus_tag="KRH39_001429"
     CDS             25651..27369
                     /locus_tag="KRH39_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG8511346.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(27451..28605)
                     /locus_tag="KRH39_001430"
     CDS             complement(27451..28605)
                     /locus_tag="KRH39_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8511347.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKRIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            29104..30465
                     /locus_tag="KRH39_001431"
     CDS             29104..30465
                     /locus_tag="KRH39_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8511348.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVAIGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALDSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAIYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            30556..31260
                     /locus_tag="KRH39_001432"
     CDS             30556..31260
                     /locus_tag="KRH39_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8511349.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
BASE COUNT        12008 a         3640 c         5541 g        10107 t
ORIGIN      
        1 gtctaaatag agctgttttt aagcaaagtt ttataaatga ctattaaaat ataaagtaac
       61 agtataaagt ttacttataa attaaatgta aacctaaatg attataatag ttgacaaaaa
      121 aatgtcttta ttttatacta aaacaaagct ttaatagaag ctttgtatat gtacatataa
      181 actaaatgaa ataagacata tgaagggaat tataaaaatg aaaaatacta aagtaaatat
      241 tcaagattta actaaaatgt caatatgtgt agcattattg tgtatatcat cttacatata
      301 tataccattg ccatttactc cagcaggagt aacagcacaa acaataatga taaatttaat
      361 agctttgatt ttaacaccaa gacaagcatt tagtacagtt ggtgtatata taatgatagg
      421 tttaattggg attccagtat ttggtggagg tacatctggt attggaaagt tgttaagccc
      481 aacaggtggt ttttattttg ggtttttgtt tgcagcacta attattagtt tattaaaagg
      541 acgaaacaat gatattaaga gatatatact aataacaatt tttgttggaa tgcctattat
      601 atattttatg ggaacagtat ttatgtgcta ttttaatcaa atgacagtag aagcagcact
      661 ttttgcagca gtagtaccat ttttaattgg agatacaata aagtcggtta ttgcatcttt
      721 tttaggcact aaactgaaca atgtactcag aagaaatcta tgataaatct ataatataag
      781 gaagggtgta atgtatggag gaagttttta tattaggagg attgagaagt catataggtc
      841 taaaaaatgg aatttttcaa tttgttcaac ctgaattact aggagcaagt gttttaaaag
      901 atttaattaa aaagtatgaa attgataaaa ttgatgagat tatatgtgga aatgcagttg
      961 ggacaggagg gaatatagct agattaatga ctttaacagc aggagtgtca aatgaagtac
     1021 ctgcttttac tgtggatatg cagtgtgctt ctgctatgat gagtatagac atagcttttt
     1081 caaaggttaa atctggtcag tgtgatttaa ttattgcagg tggttttgaa agtagttctc
     1141 tacaaccaat gcgcacatat cataagaatg ataagcgata taatacaaat aatccaaatt
     1201 atacagttgc tcaattttct cctgatgata atagccaaaa tagtatgctc gaaggtgcgg
     1261 aaagagtagc agagttatat caaattgaga aagcagactt ggatttttgg gtaaaggaaa
     1321 gtcataaaag agcaaaagaa gcaagagagg aaaaaatact tgaagatatt atatctccta
     1381 taaataatag tacctatgat gaaggtatac gtgataagat gagtcaaagg ttattagata
     1441 ggatgccatc aattttagga aaagaaacta ttacaaatgc agcaaattca tgtcttatta
     1501 atgatggagc ttcatttatt ataatatgtt ctaagaagta tttagaacat gtaaaaaaga
     1561 agcctaaagc aaaaattatt aatacatgca caataggaac agatgcaacc ttaagtccaa
     1621 catcagcgat acaagcaatg gataaacttt tagagataga aagtcttaat tatttagatg
     1681 tatctgctgt tgaatttaat gaagcatttg cagttataga tgtgttgttt caaagaaaat
     1741 atccagagtt aatagataga tataatatat ttggtggagc tttagcgtat ggacatccat
     1801 atggtgcttc aggtgcaatt attgcattac atcttttaaa agcattagaa aagactaagg
     1861 gtagatatgg aatttgctct atagctgcag ctggtggtct aggttcagct ttactgcttg
     1921 aaagggtgta gattatgaac tattatgaac ttttaagaat aaaaaaagag aggcataaaa
     1981 ataaaaattt tttaattata gatggagaaa aatatactta tgaaaatata ttgagttata
     2041 gtgaggaact aggaaaacaa atttctttag gtgaaaacat cccaatttta atatattcta
     2101 aaaatataat gtttcagttg attagttttt ttgctattaa ttattcaaaa aatattccta
     2161 ttatttgtca ttataatctt tctaaaaaag tattagatga tattttatta aaaaataata
     2221 tttcaatcat tatatctgat gaatctatgg acgttattga tttatatgat gataacaata
     2281 atgaagtagc aaaaaaagtt aatttttcta tttttaatcc taaatttaat atatatatgt
     2341 atcaatataa gaagcttata aattatttag ataaaagtat ttgtatggga gctttatctt
     2401 caggttctac aagtgttcca aaggtattat atagaacata tgagagttgg gctggatttt
     2461 ttcctataca aaatggtatt tttaatatct ctggggaatc aatcttgttt gttaatggaa
     2521 cacttagttt tacagggaat ctaaattcta taatgtcagt attatatgaa ggaggtagta
     2581 ttgtaataag ttcaggtctt aattgtaaat catggataag gacaataaaa caatataatg
     2641 taactaacat ataccttata cctacaaaac ttcaactttt ggttaaacac ttaaaagaac
     2701 cagtactcag agttgtaagt atttttacag ggtctcaatt attatttgaa gatactgcaa
     2761 gaaatctaaa aaaatatatg ccaaagtctg aaattatttt atactatggg gcaagtgaat
     2821 taaattatat tacatattta tgctatgatg aactaattga aaaacctcta agtgtaggca
     2881 gaccatttcc aggaatagat atatatatta gagaaggaaa aatttttgtg aatacagaat
     2941 atgcagtata taatgcaacc aaaccttatt ctgtcaatga tattggatat tatgataatg
     3001 atggatactt aatatttgaa ggaagaagtg atgacattat aaatattgga ggatttaaag
     3061 ttagttctac taagattgaa aatgaagtta aaaaaattcc tcaaattgaa gatgcaattg
     3121 tattgccgta ctcagacact ataagaggaa atcaaattgt attatttgta ataactatag
     3181 acaaaattac gaagaaagat ttgttgatta aaatgaaaga taatctaatg aaaaatgaaa
     3241 tcccaaagaa aattatcttt ttaaattcat ttccatatac ctcttctgaa aagatagata
     3301 gattagcatt attaaaaatg ttataagtat aatatttagt aattttagtg aaccaatgta
     3361 ttgataaaaa catattgtat atagtaaagg aggcttacaa tatgtcaaat caatacgata
     3421 taactgttat tgggggagac ctgcgacaag tgtacatggt aaaaaaacta attaataaag
     3481 ggtattctgt agtcacttat gggatatcaa atgaaattat aaatggaatc gttggaaaat
     3541 caacttcact agctgaagca ctatccaaaa gttccattat tttatgtcca attccattta
     3601 caaagaatca agttaatatt gaaaatacgg atgattgtct tgatgctact attgataact
     3661 tgctaattaa tttatctcct aatcaaatat tatttggagg taacatacca actaaagttt
     3721 gtaaattttg cgagcaacac tctattcaga tttatgattt tatgaaaatg aatgatgttg
     3781 ctattttaaa tgcagttgct acagctgaag gggctatagc agaagctatt aaaagaagta
     3841 taatcaatat acatcaaagt aactgcatag tattaggtta tggaagatgt gcacaaatac
     3901 ttgcaaaaaa attatacgct ttggatgcaa atgtatgtgt tggcgcaaga aataatgatg
     3961 caagaagtac ggctaatgca tttggatatt ccagtatttc tttaagtcaa cttgatgaaa
     4021 aacttttaca atttgattat atttttaaca ccataccaga gtgtatttta acgaaagaaa
     4081 ggttagaaaa agtatcacaa gaagtaacta ttatagatat agcttcttct cctggaggag
     4141 tagattattc tgttgttaaa gaagcaggga ttaatgcgat tctttgcctt gggcttcctg
     4201 gtaaatattc accaaagaca tctggtgaaa ttcttgttaa cgctatagaa aatattataa
     4261 atgaaagaag ggattagaaa tgagacttga aggacttaca attggagtag gatttactgg
     4321 ttcgttttgt acttatgata aaatatttat agagttagag aatttggtta aagaaggagc
     4381 taatgtacac actatatttt cagatgtatc acaaaatatc gattgtagat ttggaaattc
     4441 tgaagagttt atgaaaaaag catatgaact tacaggaaat aaacctatag ttacgattga
     4501 agaggcagag ccatttggac ctaaaggaat tgctgatatt ataataatag ctccttgtac
     4561 tggaaatact gctgcaaaat tagcaaatgg tattacagat tcaccagtat taatggcagc
     4621 aaaaggtcat cttcgtaatg ataaaccact agtaatatca atatcaacga atgatgctct
     4681 aagctttaat ttcaaaaata ttggcattct ccttaattct aaaaatatat attttgtacc
     4741 ttttggtcaa gataattgca aagcaaagcc taattctatg attgcacata ctgagctaat
     4801 aattccaact atagaacttg ctctagagaa caaacagcta cagcctgtta taaaaagtcc
     4861 acatcaatag aattggttct tataatatta tgtattagat aaaatactag caaagaggct
     4921 gtctcaaaat agagagaagc ctctttttct tgttgaaatg atgaaaatat atttaatttc
     4981 atacaaaaaa atgtctcatg gactaaaaag tctattttga gctaatctcc gtaatttttg
     5041 gtatattaaa attatttact taatccaatt tttaaattag aatttatatc gttttgactc
     5101 ttcaaatatc aatctaactg ttgttgatac aaaagaagag gacataatta aaacaaatac
     5161 cgatagtcct ctaataaaca aattatctac ataataagtt actattccaa tacctccaat
     5221 cgttaaaatt acacttataa tcaaagaaaa aatgctaatt gtttttaatt ttttcatgct
     5281 tatcctcctt cttataaact tcaaatttat ctatataata ttaaatatat tttatttcat
     5341 agtatataaa aattatgaat atatattttt ttaattatac tatatgttgt aaatgtttgt
     5401 acatttaact atatttactt taacttgtta attaagtttt aattttcatt ttcaaggatt
     5461 tttattttta aacttgatag agtatcatat taaaaattct ttttataaag ttttatttgc
     5521 aaaaaataca aaaatataaa cgttaaaaaa ataacgttat ccttaagttt ttagatgatt
     5581 ttattagtga tttgcgtaaa aaataaagtg atatctagtt aaattaagta tttgtaaagc
     5641 tagatattat tagtagaaat gatgttatat tagataaaaa ataaaatgtt aaaaaaataa
     5701 cttgaaaata gaaaaagtaa atgctataat gttaataaat taacataata aataaaaaac
     5761 tatctcagga ggttgttaaa atggctcata aagaacaagt aataatagaa aataaaaaac
     5821 aaaatgaagt tatgtcagtt ggtttagata ctgtaaataa cgttgaaagc ttagttaaaa
     5881 aactagcaaa aatcagagaa gctcaaaaaa tatttgctac atatagtcaa gagcaggtag
     5941 ataaaatttt tttagcagca tctttggcag ctaataagca aagagttcct ctagctataa
     6001 tggcacaaaa agaaactggt atgggaattg ctgaagataa ggttataaag aatcattatg
     6061 cctcagagta tatatataat gcatataaag aaactaaaac ttgtggtgtt atagagaaag
     6121 acgaagcatt tggtatgact aaaattgctg agccaatagg tgttattgca gcagttgtac
     6181 ctacaactaa tccaacttct actgctatct ttaaagcact tttagcttta aaaactagaa
     6241 atggaataat tttctctcca catccaagag caaaaaattc tactatagag gctgcaaaag
     6301 ttgttcttga ggctgctgtt ttagctggag ctcctgaagg tataatagga tggatagatg
     6361 agccatcttt agaattaact acaactgtta tgaaagaagt ggatttgacc cttgctacag
     6421 gaggtcctgg aatggttaaa tcagcgtatt catctggaaa accagctatt ggagttggtg
     6481 caggtaatac tccagcaata attgatgata gtgcagatat aaaaactgct gtaaactcaa
     6541 ttcttgtatc taagactttt gacaatggta tgatatgtgc ttctgaacaa tcggttatag
     6601 tattagaaaa tatatacaat gaagtcaaaa aagaatttaa agagcgtggt gcttatttat
     6661 tagataaaga tgaaacagaa aaaataagaa atataatttt agtaaatgga ggattaaatt
     6721 ctaaaatagt tgggcaaaca gcttgtacta tagcaaaact tgcaggcttt gaagtcccag
     6781 tagatactaa ggttttaata ggtgaagtag aatcagttga aatagaagaa gcttttgctc
     6841 atgagaaact ttcaccagta cttgcaatgt acaaggcaag tgattttgat gatgcagtta
     6901 gaaaagcaga aaaattagta gaagatggtg gatttgggca tacatcttca ttatatattg
     6961 atgatgtaaa tcaaagagaa aaacttgata agtttacttc agccatgaaa acttgtagaa
     7021 ttttaattaa tactccttct tctcaaggtg gtatagggga cttatataat tttaaactag
     7081 ctccttctct tactttagga tgtgggtctt ggggtggaaa ttcagtatct gaaaatgtag
     7141 gagttaagca tttacttaat attaaaacag tagctgagag gagagaaaat atgctttggt
     7201 ttagagcacc agaaaaagtt tatttcaaaa aaggttgttt aggagtagca ttaaaagaac
     7261 ttaaagatgt aatgaataag aagagagctt ttattgtaac agatacattc ctttacaata
     7321 atggatatac taaagcagtt actgatttat tagatgaaat gaatattaag cacactacat
     7381 tctttgaagt agaaccagac ccaactttgg aatgtgctaa gataggcgct aaagctatga
     7441 gagaatttaa tccagatgta ataatatcta taggtggagg aagtgcaatg gatgcaggaa
     7501 agataatgtg gacattgtat gagcatccag atgtagattt ccaagaccta gctatgagat
     7561 ttatggatat aagaaaaagg gtatatacat tccctaaaat gggtgaaaaa gctaattttg
     7621 tagctatacc aacatcagca ggtactggtt cagaagtaac accatttgct gttataactg
     7681 accaagatac aggagtaaaa tatccattag cagattatga actgatgcca aatatggcta
     7741 tagttgattc tgatatgatg atgaatatgc caaaaagctt aacatctgct tctggtatag
     7801 atgctttaac tcatgcatta gaagcatatg tatctatgct tgcaaccgat tatacaaatg
     7861 gattagcttt acaagctatt aagagtatat ttgaatatct tccacgtgca tatgataatg
     7921 gtgcaaaaga cccagaagct agagaaaaaa tggcaaatgc ttctacaatg gcaggtatgg
     7981 cttttgcaaa tgcatttttg ggtgtttgtc actctatggc tcataaatta ggtgctttcc
     8041 accatgtacc acatggtgtt gcaaatgctc ttttgataac agaagttatg aaatttaatt
     8101 cttcagatgc accaaagaaa atgggagcat tctctcaata taaatatcca gaagcattaa
     8161 agagatatgc tgaaatagct tcatttttag gattaaaagg aaattctgat gaagagaaat
     8221 tccaaagttt attggtagct attgaagatt taaaacttaa agttggtatt cctaaatcta
     8281 taaaagagtt tggcgttgaa gaatctaagt ttatggattc aatagatgaa atggtaattc
     8341 aagcatttga tgaccaatgt acaggagcaa atccaagata tccacttatg aatgaaataa
     8401 aagatatgta tttaaattca tattatggaa gataagtttg ctataaaata gataaaataa
     8461 aaactcttat acataaaaca aagttttatt gtataagagt ttttttgttt gagaaaatgg
     8521 atagttttta ttctttaatt taaaattcaa ttaaatagtg ttattataat aataaaataa
     8581 tgtttttatg gttattatgt tgattttatg tgtaacattt aagaatagtt atatacaata
     8641 taaatgatga attggtataa aaaggatata ttatctatat gaagtattga ctaattaggg
     8701 gaggagtcga atgacaggga gaaataaaag ttttacatta attacaattc tttttttaac
     8761 atcattattt ttgacaatgg ggtatatcta tatagaagat acaaaacatc ttcttatgtc
     8821 agaggcaaaa atacatataa aagaagttgc agtacaaggc tctcaactag cacaaagaca
     8881 aattgaaaaa gatttagatg ttttaaatat tttttctaac tattatgctt caaatccaga
     8941 tatacctaat gaggaaaaaa tgaaaaatct tttagatgaa atggaaaatc aaaaatttta
     9001 cactatggca atagttgata taaatggtaa tgctgaaaat accaaaggtg ataagttttc
     9061 agtgaaagat agagaatttt tcaaaaactc aattaaaggt aagaagtatg tttcaagtcc
     9121 atatgttgat gaagtaaata agtctataaa gaagattgct atttcagtac cattattaaa
     9181 taatgataaa gtagttggag ttttatattg tacttacaat atcaatactc tgatgaaact
     9241 tattaatata tctttttatg aaaataatag tatatcttat gttgtaaaaa ataatggtac
     9301 aattatcctt catccacaag gggatagcct atcaaaaaat atttataaat tgttaaaaca
     9361 ggataatgat attcaagaag taaatagatt aaaaaaagaa ttgcaagaaa ataaaacagg
     9421 tgcaactgta ttaaatatgt tggaagaaag aagatattta ggatatgcca ctatggataa
     9481 tgggaatagt gaaaataatt atataaaaga ctggaattta attttttcaa ttccagaaac
     9541 tgttgtattt agtaattcaa aacaaataat caacagagct gtatatgcag tgttatctat
     9601 tgtattaata tttattgcaa ttatttttta tataatttat ataaaaaaat ctaatgaaaa
     9661 agaaatatta agtttggctt atgaagataa agttacatat ataggaaatc aaaataaatt
     9721 ttatagagaa tgtagtaagt atcttttaga taaaccatct ttaaattata ttatagtata
     9781 ttttgatatt aataacttta agatgattaa tgatactttt ggatatgaat ttggtgacaa
     9841 tttattaatt acaatagcaa aagctttaaa agaagaatta acagaaggtg aggtatatgc
     9901 acgactttca agtgattatt ttgcaatttt ctgtgattat aaaaatggaa gaaataaaat
     9961 tataagaaaa cttgataata tacgaagtaa tattgaaagt aatttgagta tagtctttga
    10021 aatttctctg tgtgtgggta tttattttgt tgaagaaggt gaagtagata ttcaaaaggc
    10081 tgtaaataaa gcaaatatgg ctaggtctgt tgctaaaggt aaaaatataa attatgctat
    10141 ttataatgaa gatgttagga ataagcttag tgaggaatca atgattttag atgatataaa
    10201 aatagcattg gtaaaaaatc aatttgaagt gtattatcaa cctaagtttt catttgtaac
    10261 tggagaaatg atagggagtg aagcactaat aagatggaat catccagaac atggttttat
    10321 aagtcccgca gtatttattc caatagcaga gaaaagtaag ttgattttaa aaattgggag
    10381 atttgtattt gaaagggttt gcaatgattt atatgaatgg aaaaagcaag gtaaaaagat
    10441 agttccagtt tcagtaaatt tatctagggt cgagttgtat caaccagata ttgtaaaatt
    10501 tatcaataaa acaataaaaa tgtataattt aagttcagat ttcattgaaa tagagataac
    10561 agaaactgtg gctattaatg agttaaatat tttaaagaat gttttaaatg agttgaggac
    10621 gtatggattt tctatttcta tggatgactt tggtacagga tattcttcta ttagttgttt
    10681 aagagatatg ccaattgata tattgaagct agataagtct tttcttggtg gcattgaaca
    10741 tgatgagaga agtcgcaata tagcaaaatc aatagtgtct ctagcaaaat cactagattt
    10801 agttgtaatt atagaaggtg ttgagagtaa agaacaagct gagttaatga aacaatttgg
    10861 atgtgattta gttcaaggat tttattttgc acgaccaatg ccagctaaga attttttaga
    10921 tttactttaa aacaaaattt gatttttata taaagcacta ttatgaatgc ttgattttaa
    10981 taaatcattg ctaaataatt agtgtttttt tattttatca atgtattgtc taatataaag
    11041 caaaaaataa ttgtatttta taaaaattat attaaaaata aatttatttt gctaataaac
    11101 atgttattaa caaaagaata gtactataat gtatagatga taaaaacttt aataaaaatt
    11161 tagtgtatag gagttgttta agtatgatag atataagtat tgatgacaaa cttaaggaaa
    11221 gatggcctaa tgctaagctg ggttgcattc aagcaaaagt agcagtaaat aaaagctcag
    11281 aaaaattaat taatgaaata aatgagtttt gtgatactat aaatcaaagt ctaaaagttg
    11341 aagacattac gaaactagat aaaattaaag atgcaagaaa tgcttataaa gagcttggaa
    11401 aaaatccaag tagatataga acatcttcag aagcattggt tagaagaatt attcaaggga
    11461 agggtcttta tacaataaat aatatagtag aaataaacaa tttaatatca ataaaatcat
    11521 tatatccagt aggtacatat aatgtaagca aattacattc accagtatgt tttactgtag
    11581 gagatgaagg agaacagtat aagggtattg ggaaagaatt aataaatata gaaaatcttc
    11641 ctatattgtc tgattcagtg ggtaaatttg ggagtccaac tagtgattct gaaagagcaa
    11701 tgataacaga agatgcaaat gaaatattaa tttgtatatt ttcttttagt caagaatctg
    11761 atattgaaga atattttgga tatgctaagg aattacttaa agaatatgca gatggaaaag
    11821 atatagaaac taaaataatt aaatagaatc tatataacaa cgttatgtta agctaaaatt
    11881 caatataaaa ataagttaag ataaagaaac taacacaaaa ttagtttctt ttttaaaata
    11941 gtagtttagt gttacaaaaa tagatagata gtcccacgat tattaaaagt atggtaatat
    12001 attaatatag cagagattgt gtttaaggga ttttgaaagg ggggatttat aataatcatt
    12061 ttatctaaaa gataaaatgg ttttatagaa ataactatat atttaaaaat ataaataatg
    12121 atacagtaat ttgggtatat aaaataaggc atacatggtt ttgtttaaaa accaataagg
    12181 tacataggta attacaatgt aaggaaggat ttaattatga ttgatggaaa agaggtaaaa
    12241 caagaaagtg gacaaaaaaa tctaaaattt aagatagcta tagtagtagg tgttttgttt
    12301 gtagcttata taggaatggc aatctttttt agaaatcatt tttactttgg aacgactatt
    12361 aatggcgtaa aggcttcagg aaaaacagtt gagcaagtaa atgatttgtt agcatctagt
    12421 actaattcct atactttaaa cttagaggaa agaaacaata agaaagaaca aataaaagca
    12481 aaagatattg atttaaaatt tacaccagat gataaagttc aaaagataaa agatagtcag
    12541 agcgctattg gatggattgg tggaatattt aaatctaaag agtataataa tatgataact
    12601 ctttcatata ataaagattt actagaaaaa aggtttaatg ctttatcttg ttttgatagt
    12661 aaaaatgttg tagaacctaa gagtgcttat cctaagtatg atagcactaa aaacgcttat
    12721 gttatagtac cagaggtact tggaaacaaa gtgaaaaaag atgattttta taagttagta
    12781 caaaactcaa ttaattctgg gaatacttct atttctctgg atgaaagtaa agtttatgaa
    12841 gcaccaaaca acacaaagga ttcagaaaag ttgaaacaag ctattaagac attagataag
    12901 tatgttggtg tagtcgttac atatgatttt agtgaccgta aggaaacttt agatggttct
    12961 gaaattaata aatggttaaa agtagactca gaaaagaatt atgacataac ttttgataat
    13021 gatgctatgg tagaatatac aagaggttta agtaaaaaat acagtacttt tggtgactca
    13081 agaccattta aaactgccac aggtaaagat attacaattt caggtggaat ttatggttgg
    13141 ttgatagata aaaagaaaga agctgaagca ttagttgatg ttgtaaaagc tggtaaaaat
    13201 gttacaagag aacctattta catacaaaaa gctgtatctc gtaataaaga tgatattgga
    13261 aatacatatg tagagattag tctttctggc caacatatgt ggttctttaa aaatggagaa
    13321 atgcttgtat caacagacgt tgtaacaggt gatttagcta gaggatttgc tacaccatca
    13381 ggtatatatc ctcttaatta taaggctaga aatgtttcat taactggtca aggttacagt
    13441 tcacctgtaa gttattggct tccatttaat gggaacatag gcattcatga tgctacttgg
    13501 agaaattctt ttggtggaag tatatataag tcttcaggtt cacatggatg tgtaaataca
    13561 cctttttcta aggcaaagaa aatatatgaa aatatagaac caggtacacc aattatttta
    13621 tactagtaaa tgtgttgcta acagttaagc gaagtcatct tagtttattt aggatggctt
    13681 cctttttata taaagcttat tgaagtttac agaaagacta cttaaaagga gaaatacata
    13741 atgaatgaaa aattaactaa aattttagaa gaagctaaga atgatgatga atttagaaag
    13801 gaacttataa aaacaagaga acaacgtgat gttatggatt cattttgtaa ggttgttaca
    13861 tctagaggtt atgatatgac agtaggggaa cttttttcta tgggagaaga atatacatcc
    13921 aatctactga aaagtgtaaa tggaggagca gcatatccta ttgaagattg gagtgattgg
    13981 tacgaggatt tttttataat gttgtcgaat atatagagta actaatgata ttatctactt
    14041 aagattaata cattgcctta aaataaaact attagaacta ggtgtaagtt ttatcaatct
    14101 ttaaaaatat tgatttattt ctaactagat ataaaaaaat tagattaaaa ataaggtgac
    14161 aaaaatcgat atagcttgat gatttataaa agttagataa aatagtgcga gatatttcta
    14221 aaaattcaaa aaaaacaata aacttcaatg gttagaggtt gaaaaagaat ataaaaaact
    14281 tttatatgta ataaattaaa ttaaaattat aaaaaaaata aaataaaaat ttaaaatgtc
    14341 gacataacgt taattttaaa acaaaataac gctattgaca aactatatcg ctttaaatta
    14401 taattattga aaataatggt atttaaaaac catactcatg tcatagtcta aaagtaagtc
    14461 aaaaaaggat gtaaatctag gcaagtacta ttctaaaata tgttttatta agaaataaac
    14521 gttgaaaaat attaattatt atataaaaat gctgtttata ctttaacaat gaggtagtaa
    14581 tctaattaga ataaaaaaat ctaattggtt atatacatag taaaaaggta agagaggtga
    14641 tttggatggc ttttgaaata ataaaaagca ttgttgaggc agagcagaca gcagacagta
    14701 tcaaagtaaa agctgttact gatgcagagt ctatcagagc tgatgctgta aacaaatgtg
    14761 aaagcatatt tgctgatgta aaaaaacaag caaagcttat ggaagaaact cttattgaga
    14821 aggcagtcac cgacagtaga gcagaggttg ataaaatctt agctaatgct aaaagtgaat
    14881 gtctgaaaat tgaaaaaact gctgaagaaa gaaaaagtaa ggctattgaa gcggttattg
    14941 gaaaggtagt gagataatat ggcaatagtt aatatgaaca aaatatctat tgttggctta
    15001 gaagctcaaa aaagccagat tttaaagcta cttatgaacc gtgggtttgt tcagattgat
    15061 gatagtgcct ttcttactga ggaggaggaa ttaaaaaatt atcttgtaaa agatagtgaa
    15121 gaatctgaag ttataatgct agagcagaaa atgtctcatg taaaccaagc aattaatatt
    15181 gtttcttcac tagtgaaaca aaaaaaatcc ctacttgcac caaaaagaga gtttaacaaa
    15241 attgatagtg aaaaagaagg gcaagtttat gctgaagctc tggagttaaa taatttgcac
    15301 aaggagatag gtacaataag gtctaacata aacttactta acaacaataa aagtatgtta
    15361 gaaccatggg tgaactttga tattcctatt gaaaatatgg aaactaagta tacaaaaact
    15421 atacttggaa ctgtgcccat aagcgcaaac attgatgctt taagagtaaa attagaagat
    15481 gaaataccag aaagtgttat tgggattgtc aattctgaca agttaatgaa ctatgtttat
    15541 ttaattactt taaaagaaac ttttgatgat gttttagaag ttttaaaaga atttaatttt
    15601 tcagtggttt cattaccaaa tgaagaaggt acaccgttac aagctatcaa gaaatacaat
    15661 gaaatgataa ttagacagga aaacgtttct aaggacaaaa cagaggaaat taaaagacat
    15721 gcagatagta tacacatgct tgaaaaccta tatgattttt acactataga aagagaccaa
    15781 aaaaagatta tagagagact tgtcaaaaca aaaacaacat tctgtcttaa tggttggtta
    15841 ccaagcaaaa gggcagatga attaattaaa gaaattacag aaaagtttga ttgttgtgtt
    15901 cagactgaag aaggaagtaa agaagaaggc ttcccaattc tgcttgaaaa caataaatta
    15961 gtaacacctt ttgaaagtgt aactaacatg tacagctatc caagtacaaa agatattgac
    16021 ccaaatacaa ttttaacttt cttctttgtt gtattctttg gaatgatgct aagtgacgcc
    16081 gcatatgggt taataattgc agtagtttgt ggatttgttg tatataaatt aaagatacaa
    16141 aaaggtgaag gaaatttaat taaacttatt ggtatctgtg gagtttcaac tactgtatgg
    16201 ggtcttatat tcgggggtat tttgggggat ttaattccta taaaagcatt aattaatcct
    16261 ttagaggatg ttatgacact tatgggaatg tcgttactgt tcggtattat ccatatatat
    16321 gtaggtttgg gaataaaagc atatacatta ataagaagtg gaaaggtaat agatgcaatc
    16381 tttgacatag gtttttggta tttgtgtata acaggtgtat gtctacttat aatacctttt
    16441 gttgcagggg acataggtgt attttcagaa gtaggaaaat atttagcaat tataggtgct
    16501 ataggtcttg tacttactca aggaagaagc tttaaaggta ttcctgttaa gctttttaaa
    16561 ggattttcaa gtctctatgg tataacaagt tattttgctg atatattatc ttatacaaga
    16621 attatggcgc tttgtttaac aacaggagtt atagcacaag ttatcaacct tttaggagct
    16681 atagctggtc cgatacttgc agttgtaatt ggtgttgtag gacacacaat aaaccttctt
    16741 ataaatgcac taggtgcata tgttcataca agtaggttac agtatgtaga atttttcaat
    16801 aagttttatg aaggcggagg agttcctttt gttccgttta agtataaaac aaaatataca
    16861 agcattaata aaaaggagat gtaaatatgg aatttttaaa cgcgttagga aatggtcaat
    16921 ttttggcaat atcaggagct gctatagcag cattatttgc aggtatgggt tctgctaaag
    16981 gtacttcaat agcaggtcaa gccgcagcag gtgttgtaac agaagaccct agtaaattcg
    17041 gtcaacttct agttttacaa cttcttcctg gtacacaagg tctgtatgga tttatcatag
    17101 ctttccttat tttatcaaaa gcagggatta ttggtggaga tgcaatacca acatcagctc
    17161 aaggtttaca acttcttatg gcaggacttc caattggttt agttggttta gtatcaggta
    17221 tagcacaagg taaagcagca gcagcaggag ttggaatagt tgcaaaaaga cctgaagaac
    17281 ttggtaaagc tataacattt gcagcaatag ttgaaacata tgctctttta ggactacttg
    17341 tttctttctt agcttacaat ggaattgcaa ttggttaatt ataaattata attcataagt
    17401 aaaaaaaatg aaaaggagga agcactccta tgggtaatga acaaaaaatg attgacagaa
    17461 ttatagctga tgccaaacaa gaagcacaag aaatattgga taaagctaaa agtgaagcag
    17521 atttaaaagt aaattcagct aatgaaaaag ctgaaaagga aatggcttcc tatacaaagc
    17581 ttgcagaggc agaagcagaa aaagcggctt ctaaggaaat atcaggagct tatatggagg
    17641 ctaaaaagca aatattatca aaaaaacaag agattttaga agaggtaatt ttagaagcta
    17701 aaaacaaact tttaaatctt aaagataatg aatatgaaga gattatacta aacatgattg
    17761 aaaaatcaaa ttgtacagat gattcagaga tagtattatc taaaaaagat aaaaaaacat
    17821 taaaagatgt gctatcaaaa aaaggcatta aagtgtctga tgaaactaga gatattactg
    17881 gaggtttcat agttaaaaaa ggtgacattg agtacaatta ttcatttgaa gccataattg
    17941 cagtagagca tgaatatatt gaacaaattg cagcagaaat tttgtttaat taaggagggg
    18001 ttaaaaaatg gcagaagaaa aaacttatcc ctatgccgtt gcacgaataa gagtgcttga
    18061 aaaacagctt cttaacagac aaatgtttat tcaaatggca gaagccaaaa gccctgaaga
    18121 ctctttaaga attatagctg aggcaggtta tgctgataca tcaaataata atatacataa
    18181 ttttgagaat attttgactc aagaattatc aaagacatat gaaattctta aaagtctagc
    18241 tcctgaagaa aaatttgttg atgtatttct ttataaaaat gactatcaca atttaaaagt
    18301 gctgattaaa gaagaaattt ctggtgtaga tggtgagaaa tatttaattg atggaggtac
    18361 aattcctctt attaagctta gagaatcttt agcaaatcgt agttttagtg atttacctaa
    18421 gataatgtca tcagctatat tagaggcatt tgatgtttat aataagacac aaaatggcca
    18481 gatggttgat attgttctag ataaagcaac ctttaccagt atgaaagaaa ctgctaaaga
    18541 aagccaaaac aaatttgtaa tagattatgt tatgaaagtt tgtgatttaa caaatctaaa
    18601 gagttttata agagtcaaaa atatgaagaa agattttgat atgtttatga atgtgtttgt
    18661 ttcaggtggc tcacttgata aggaaaaatt cttagaagct ttttcctcag atactccagc
    18721 atcatgtttt aaatctactt cttactctga tgtctgtaaa aatggcatgg atagtggttt
    18781 tactgtcttt gaaaaacttt gtgatgacta tcttatggaa tatgttagag gagcaaaatt
    18841 taagcctctt acattagaac cactaattgc atatttatat gctaaagaat cagagataaa
    18901 aacaattaga attattttga ctagtaagct taataatata gacgcagata ctataaaaga
    18961 aaggctaagg gatgcttatg tataaggtag gagtagttgg agataaagat agcatcatgg
    19021 gatttttagc tcttggaata gatatcttcc cagcctatga tagtgacgaa attaaaaaaa
    19081 gtatacataa gttggttgaa gatgaatatg cgataattta tattacagaa caggcatcat
    19141 tgcttgcaaa agaatcaata gcaaaataca aagattacca acttcctgcc attatagtta
    19201 ttccaggtat tggaggaagt atgggattag gtatgaatga agtaagagaa tctagtaaac
    19261 gtgctatagg tgcagatata ttatttaaag aatagatgaa agttgaataa aagacaatct
    19321 caaattttat tggtgctaaa tatatgtgct aataaaatat aggagggaat atttaaattt
    19381 tgataaagta tatgattgca ggtgagagaa tatgaaaaca ggcacaattg taaaggtgtc
    19441 aggaccgctt gttgtagcgg agggcatgag agatgcgaat atgtttgacg tcgtaagagt
    19501 ttcagataaa catcttatag gcgagattat agaaatgcat ggagataaag cttctataca
    19561 ggtttatgag gaaacttccg gtctaggacc aggggaagaa gtagtttctc ttggaatgcc
    19621 tatgagtgtt gaacttggac caggtcttat ttcaactatt tatgatggta ttcaacgtcc
    19681 acttgaaaaa atgtatgata tttcaggtac taatataact aaaggtgttg aagtatcttc
    19741 acttgataga acaactaagt gggaatttaa acctaaaaaa tctgttggtg ataaggttgt
    19801 agcaggagat ataattggtg gcgttcaaga aacagcagta gtagaatgta aaattatggt
    19861 tccttatgga gttaagggta ctataaaaga aatatattct ggtgaattta ctgttgaaga
    19921 tacagtttgt gttattacag atgaaaaagg taatgatata cctgtaacta tgatgcaaaa
    19981 atggccagtt agaaaagaaa gaccatatag agaaaagaaa acaccagatt cacttcttgt
    20041 tacaggtcaa agagttatag atacattttt cccaatcaca aaaggtgggg tagcagctat
    20101 accgggacct tttggaagtg gaaaaactgt tacacagcat cagcttgcta aatgggcaga
    20161 tgcagatata gttgtatata taggctgtgg cgagcgtgga aatgaaatga cagatgttct
    20221 tcttgaattt cctgaattaa aagacccaag aacaggcgag tcacttatgc aaagaactgt
    20281 gcttatagca aatacatcag atatgccggt tgctgcacgt gaagcttcta tatacactgg
    20341 tattacaata gctgaatatt ttagagatat gggatatagt gttgcactta tggcagactc
    20401 tacatcaaga tgggctgagg ctcttagaga gatgagtggt cgtttagagg agatgcctgg
    20461 tgaagaaggt tatcctgcat acttaggttc acgtcttgct caattctatg agagagcagg
    20521 aaaggtaaat tgtctaggta tggatgaaag agaaggaaca cttacagcaa ttggtgcagt
    20581 ttctcctcct ggtggtgata catcagaacc tgtcagccaa gcaactcttc gtatagtaaa
    20641 agtattctgg ggtcttgatg catctttagc atataaacgt catttcccag caattaactg
    20701 gttgactagt tattcactct atcaggaaaa agttgatgta tgggcagata aaaatgtaaa
    20761 tgataatttc tcagctcaaa gagctcaagc tatgaaactt ttacaagttg aatctgaact
    20821 tcaagaaata gttcaattgg ttggtgttga ttcactttca gatggagata gattgatact
    20881 agaggttact cgttctataa gagaagactt ccttcatcaa aactctttcc atgaagttga
    20941 tacttataca tcacttcata aacaatatag aatgatggga cttatattaa aattctataa
    21001 gtcagcacaa gatgctatca agcaaaatgt tactataaac gatattatca agctttcagt
    21061 tcttgaaaag attggtcgtg ctaagtatgt agaagaaggc gatatagatg cagcttacga
    21121 ttcaattgaa gatgaaatag acaacgagct tgcagacctt attaaaaaga ggggggctga
    21181 agaactatga taaaggaata taagtcaata caagaggtag ccggtcctct tatgttaata
    21241 aacggcgtag aaggtgtaaa atttgatgaa cttggagaaa ttgaactttc aaatggagaa
    21301 attcgccgtt gcaaagttct agaggtaaat ggagatactg cacttgttca gcttttcgaa
    21361 agctcaacag gtataaacct tgctgaaagt aaagttcgtt ttttaggaaa gagtcttgac
    21421 tttggagttt caccagatat attaggtcgt gtttttagtg gaatgggtag acctattgat
    21481 ggaggtccag aaattatacc agataaaaga cttgatataa atggagctcc aataaatcca
    21541 gcagcacgtg attatccggc cgagtttata caaactggag tctctgctat agatggactt
    21601 aatacattgg ttagagggca aaaacttcct atattctcag ggtctggttt gccacatgcc
    21661 aatcttgcag ttcagattgc aagacaggca aaagtacgtg gaactgattc taagttcgcc
    21721 gttgtatttg ctgcagttgg tataactttt gaagatgctg aattttttat aagcgatttt
    21781 aaagcaactg gtgcaatcga ccgttctgta gtgtttgtaa accttgcaaa tgacccagct
    21841 gtagagcgtg tatctacacc tagaatggca cttacagcag cagaatacct tgctttcgag
    21901 caagatatgc atgtacttgt tataataacg gatattacaa actatgcaga ggcattgcgt
    21961 gaagtttcag cagctaaaaa agaagttcca ggacgtcgtg gatatccagg atatctttat
    22021 actgaccttg ctactatgta tgaaagagca ggaaaaatga aagggcatga agggtctata
    22081 actatgattc caatacttac aatgcctgag gatgataaga cacatccaat tcctgacctt
    22141 acaggataca ttacagaagg gcaaattata cttagccgtg aactttataa aaaagatata
    22201 cagcctccta tagatgtttt accatcactt tctcgtctaa aagataaagg tattggtaaa
    22261 ggtaagactc gtgaagacca tgcagataca atgaaccagc tttttgcagc ttattcaaga
    22321 ggtaaggatg caaaagaact tatggctata cttggtgaat ctgcactttc tgagattgat
    22381 ttaatgtatg ctaagtttgc agatgaattt gagaaagaat atgtttcaca aggatttaga
    22441 actgatagaa ctattgagca gacattagaa atcggatgga agcttcttag tatgttacca
    22501 aaaagtgaac ttaaacgtat ccgtgatgaa tattttgaaa aatatatgcc taaggagtga
    22561 tatctgtggc tagattaaat ataaatccaa cacgtatgga aatgactaga cttaagaaac
    22621 tccttaaaac tgctacaaga ggtcataagc ttcttaaaga taaacttgat gaacttatga
    22681 aacaatttct tgagattgta agagaaaata agcgattgcg tgaagaagca gaaaatgctc
    22741 ttgatactgc ttataaaaac tttataatcg caagagcagt tatgagtcag gagtatctgg
    22801 gttcagcact tatgatgcca aagcaatcag tttcagtaga tgtttctacg agaaatataa
    22861 tgagtgttga tgttcctgtg tttgacttta aaactgaaaa caatcaaagt gatatttatc
    22921 cttatggtct tgcttttaca tcaggtgaat tagattctgc tatggaagct ttctcagatg
    22981 ctatgcaacc acttttaaga cttgcagaaa gtgaaaaaag tgcacagctt cttgcacaag
    23041 agatagaaaa gacacgtaga cgtgttaatg ctcttgaaaa tgtaatgata ccaaattaca
    23101 ttgagactat taagtatata gctatgaagc ttgaagaaaa tgagagagca agtactacta
    23161 gacttatgaa ggttaaggat atggtgctta aaaaagcact tgaagaaaag aaaaaaaatg
    23221 atttggttgt ataattaaat acttataaaa ataagtctaa cattctactt tatagaggtt
    23281 agacttattt tttatgttta aattaaaatg tagatactat aaaaattaat atattgttta
    23341 aaaacagact gtatttaact aaaaataata tatttaacaa aaatttctaa aaattaacga
    23401 actgttaatt tacacttaac tttatattaa caaattaggt gtattatata aacataagca
    23461 aaaacaataa aaaacaaata aaaaaaggaa aaaacacaaa ggggggaaga tatgaaagaa
    23521 tttatattta cttgtctgtg cattgtagtg attacattta tgacaagttg catatcaaca
    23581 acaccacaaa ctactcaagg aaaaggaaat aataaactta caatatatgc agcactagag
    23641 gaagagcaga tagcttatta tatggaaggt tttagagaag agtatcccaa tatagatgta
    23701 gagatagtta tggattctca tggtgtaata gcttcaaaac tattagcaga aaaagataat
    23761 ccacaagcag atattatatg gggattatca gcaatgaaca tgatagagct aaaaaaagat
    23821 ggtgcacttg aagcatataa gcctaaaaat tatgataaag ttgataaaaa atttaaagat
    23881 actgaagatg atgtgtcttg ggtaggaatg agtgtcccag agacagcaat agtagttaat
    23941 tataaggaac ttaagaaact aggtatagat attccaaaaa gttatgaaga tttaataaaa
    24001 ccagaatata aaggacttat aacaatgcca aatccagcat catctggaac gggttatctt
    24061 acagtatcag ggcttattca aatgatgggt gaaaaagatg cttacaatta catggataaa
    24121 ttacataaaa atatagccac atatacacac tcgggttcaa aacctgcgaa gttagctggc
    24181 tcaggagagt atccaatagg tattacactt ggatatagag ctacacaaca attatctagt
    24241 ggagaaccta tagaggttgt atttccaaag gaaggttcag gttgggactt agaagcaaat
    24301 gccttagtta aaaaagataa tataaaaaaa gaagcaaagc tatttttaga ttgggcaata
    24361 agtgattcag ctatggatag atatgcacat gaagtagcag ttacttccat aaagacagat
    24421 aatccagttc caaaaggtta ttctaaacaa ccatttgaac agcttataaa agaagttgat
    24481 ttaaactctg ctgccaaaaa cagaagtaca gtacttaaaa cttgggaatc aaaatacggt
    24541 tcaaaatctg aaagtaagta aagtttaaat taataattta gatgaaagaa ttttagaaga
    24601 aaaagtagga tataaaattt aaaataaaaa aataactata taaggagaga taagatatga
    24661 gttacttaaa aataaataat gtgtttaaaa gttatgacca aaaaaaggtt ttaaataata
    24721 ttagtttgga tattgaagaa ggggaatttc tatgtttact cggacctagt ggatgtggta
    24781 aaactacatt acttagaata atagcaggcc ttgaagatgt aaatagtgga actataattc
    24841 tccaggataa agatattaca aatttagagc catcaaagag aggttttggt atagtatttc
    24901 aatcatatgc attatttccg aatatgactg cttataacaa tatagctttt ccactaaaag
    24961 agagaaaagt ttcaaaagaa aaaatagata ataaagttaa ggaagtactt gaaacagttg
    25021 gattgactaa tgaagctcat aaatacccta aagcattatc aggtggacaa caacagagga
    25081 ttgcaatagc aagagcatta gctcttgaac caaagttttt acttttagat gaaccaatgt
    25141 ccgcattaga tgcaaaggtt agacacaagc ttagaatgga cattaaaaga ttacaaaaag
    25201 aacttaatat aacgacaata atggtaacac atgaccaaga agaagctata actatggcag
    25261 ataaaatagc tattctaaat ggaggagata taatgcaaat agggactcca gaagaaatat
    25321 accaaaatcc tcaaaaccta tttacagctc aatttatagg agatacaaac tgttttgata
    25381 atggagattc tatattaact gtaagaccag aatatgttca aatagagaaa tcgacaaaag
    25441 aaaactacca agggataata tcaaatatag agtttagagg gaatttattg agagttgaaa
    25501 taaaagataa attaaatgaa aatttcataa taagtgatgt ttctataaaa gagtgggtta
    25561 atttaaattt agtagaaggt gaccttgtaa aaataagtat agacgaaaaa tactatttaa
    25621 agtatccaaa agttaaagga gcataagcgt atgaataata atgctataag ttctaaaagt
    25681 ataagtctta aatcaaaaat gaatttagat ataaaaaaat gtatgcttac agtattagtt
    25741 atgtctttag taatattttt attagcacct ataatatatc tatttgtaca ggctttttta
    25801 gataaagatg gagtctttgt aggattacaa aactttcaag aatacttggc ttctgaatct
    25861 ctattaaatt ctctaaaaaa tacagtattt gtatcagtta tatcaactgt aatatcacta
    25921 acacttgcat tagtatatgc atatgcatta actagaacaa agattaaatt taaaggtttt
    25981 tttaaatcat tagctatgtt tcctatgttt gctccaacaa tgatgcatgc aataggtcta
    26041 gtatatttat ttggaaatca aggtttggta actaatttac ttggagtgca tgttccaata
    26101 tatggagcag tgggaataat attggcacaa attatatatg tatttcctca atcatttcaa
    26161 atactttata tatctctaag ttcaaccgat tatagactat atgaagctag taatactatg
    26221 gggataagca agataagaga atttataagc ataacaatcc caagtataaa atactcttta
    26281 ataagttctg cattcgtatc gtttacatta tgttttacag attttggagc tccaaaggtg
    26341 ataggaggag agtttaatgt attatcaata gatgtatata agcaaattat agggcaacag
    26401 aatatgccaa tgggagcaac tgtatcaata ttacttttaa tacctgcaat aatatcgttt
    26461 atagtaacta aaaaaattga aaaaaatcaa aattcatata taacttcaaa gtcaattcca
    26521 tataaggtta aaactaataa gttaagagat agtattttag gaggggttgt aggaattatt
    26581 gcatttggta taatagctgt aatgttaact gtaatcttat cttcattaat atcagtttgg
    26641 ccatatgatt taagactgac actggaacat ttttctatga aaaacagtat tgatgggatt
    26701 aaaccattta taaattcaat aaaaatgtca ctattgacag ctattctagg gacgagcttt
    26761 gttttcttat ttgcatattt gaatgaaaaa acagaaaaag ctccatattt aaaatcattt
    26821 gggtattttt taagtacatt gcctatggca ttaccaggat tagttttagg tatagcttat
    26881 gtattatttt ttaataaatt agagtttaat tttatggata caatatacat taagaatttg
    26941 tttaatggat tatatggaac attacttata atggttatat gtaatatagt tcatttcttt
    27001 tcagtagcat ttataacagc tactactgct attaagaaag tagataaaga attggatatg
    27061 gtagctaaat caatgggaat aaacagtgca tcaattctta agaacgtaac tataccactt
    27121 tgtttgccag cagttttaga gaactttatg tactttttcc taaactccat ggtgacaata
    27181 tcagcactag tttttatata tactgcaagt tcaaaagtag ctgcaatatc aattgtacaa
    27241 ctagatgata aggggagtac tgcacaggca gcagcactag ctgtgttaat attagtcaca
    27301 aatatattag tgaaagtaat atatgaattt ataaagaatc gtttagaaaa aagaacaaac
    27361 tttaattaaa taatttatct tataaaaaca caaatttaaa aaagtctatt cttgtgattt
    27421 attatttaaa gaatagactt tttaaattta ttattgatat tgtttattaa ttttcctttt
    27481 gttatcatac atttgagaat ctgcacgacg atatgtatca aataaagaaa catctacttt
    27541 ttcatcaaaa atagcatatc caatagacat tgataatttt aattcattag ttgagttttc
    27601 ttcaactatt tttaaattaa tattatttat aatattattt atttctttgt gagataaatt
    27661 ttttagaata attatgtatt catcacctcc aatacgataa actatatatt ttttatttat
    27721 tacatattgt ataattttac ttgcttgctt aatatataag tctcctgtct gatggccaaa
    27781 tttatcatta atctttttta aaccgtctaa atctattgat atgatagata ttagattctt
    27841 tagatttgaa ttgtttaagt tattcatatc tatttcaaaa gcatttctat tttttaggcc
    27901 tgttaaataa tctgtattag caaaatcagt atatgtagga ttagatattc ttttaaatag
    27961 aatcactgca atcaatgctg atattagaca agttaataat gctattattg gtgtaactat
    28021 acttataaat ttaaatgtat tgtattgatg ttttgcttca aattcaattc ctattactcc
    28081 aacaacctta ccaacatcat tgtgaatagg aaaatacgat ataaatatgt atccccaaga
    28141 tgttgattta atctttttag gtaacaatat atgattatct aatgctttgt taaggtctcc
    28201 ccaaatctct ttttctatca aatcaccagc atttctaaaa tcactcctag atgaatttaa
    28261 accatcaaca acatatacta gtgatccatc ttttgttttt ttagcagtat atagatacat
    28321 gactccagta gtatttttta cagattctaa aaggatttta gaacttttat aagacttttt
    28381 tgattggtct tctttagaat taatttcagt aaaggtattt ttatcaatat ttctttctac
    28441 atattcatag atacgttttg acctctcact taaagagtat atcatgctct tataagttag
    28501 tgtataatta aaaaaatata cacatgagga agatattatt acaataattg cagtaagtat
    28561 tgaaacctgc atatcaactc tattgaattt tttatttatt ttcatagctt aaatgtcttg
    28621 tgaaaagaac ctcccacaat ctccccataa ttaatatata tctataaatt tcaccataat
    28681 atattcatat attatcaaat ttgtgtggaa attacaatta gtaaacatgc ttaaagcatt
    28741 caaatatagt ttttttatta ttatagtata ctattttatg tataaataaa attatatata
    28801 aatcattgat attggttgtt tattaatgta agataccgta taaaatattc taattggtta
    28861 cgatatgtat acttaattga agttaagtta agggttagaa tatgtattga tgaaatggct
    28921 agatttatat tcataagtat taattttatc atagataggt aaaatactgt atcttaaaca
    28981 taagtatgat tgagatgcaa tcctgagata tgataatata aattgaagga tagtgaactc
    29041 aatcaaataa aatgattaat ctagctatat aagcaaataa atttaatata aaagaggtgg
    29101 aaaatgaaag acagacttaa tttaacagaa ggaagaatta cagaaaaatt acttaagcta
    29161 tcacttccaa taatggggac ttcatttata cagatggggt ataacatgat agatatgata
    29221 tgggttggta aagccggaag taaagcagta gctgcagttg gaacagctgg tttttttcca
    29281 tggcttgcaa tggcatttat tatgatttca aaagtaggtg gagagataaa agtagcacaa
    29341 agtattggtg caaataatat aagcactact aaatcatata tgaagtcagc aatagaaata
    29401 aatattatac tagcaataat atatacaatt tctttaatag tacttaataa acaattgata
    29461 ggttttttta gattaggaga cttagaagtt ataaccatgt ctagacagta tttgataata
    29521 gtagctatag ggatggtatt ttattttatt aatccagtat ttactgcaat tttcaatggt
    29581 cttggaaata gtaaaactcc ttttcgtatt aatacagtag gattaataac taatattata
    29641 cttgacccta tattaatttt tggatgggga tttgctccaa aacttggtgt tgcaggagct
    29701 gctattgcaa ctgtatttgc tcagatagta gttactacat gttttatata tataatagtt
    29761 aaaagtaaag aagaatattt taaattaaga ctatttaaag aaatagagac taaatattat
    29821 aaagttctgt ataaattggg attacctata gcaatacaaa gtggtatgtt tacagtattc
    29881 tcaatgctat taggagtaat agtagcatca tgggggcctg tggctgttgc agtacaaaaa
    29941 gtaggctcac aaatagaagc aatttcctgg atgacggctg aagggctatc agttgcatta
    30001 gacagttttg ttggacaaaa ttatggtgcc aagaagtatt ctagaataaa taaaggttgc
    30061 aaaatagcta taactgtatc ttctgtactt ggtataataa ctactttagt attagtattt
    30121 gcaggagaga gtatattttc tatttttata gatgaaagtg aggcaataga aaaaggagca
    30181 atttatctta aaatattagg ctattctcaa ttttttatgt gtttagaaat aataacaaca
    30241 ggagctttca aaggattagg gagaacctat ataccttcta ttataattac tatactgact
    30301 ggtgcaagag taccattggc ttactttata tctagaccag aaatgttggg tctaaatggt
    30361 gtatggtgga gtataacctt atcaagtata ttaaaaggta tattaatgat aagtctattt
    30421 atatctttat taaaactcgg aaagttgtac aagatttctg aataaaattt aaattatctt
    30481 aaatgtatag acattttctt tgtttgtata taaaattatc ttattatata aaagatttaa
    30541 taaggagtga attaaatgaa agagccaatt tataaagtta tagaaaatca tgttagagag
    30601 ttaataaatt cagacagttt gaaagaaggg gatttaatcc cttctgaaaa acagctgagt
    30661 gaagaattta atgtaacgag aatgactgta aggtctgcac ttaataatct agttaaagaa
    30721 ggctatataa ctagacaaag aggtgtaggt agtatagtta ttgcaaataa tatatatgat
    30781 aatatatcgt ctgtaagtgg atttactaaa gagatggaaa gtaaaggcta taaagtatct
    30841 aatatattgg tatctcttga aatagttcag gcagatgaaa atttatcaag taagttgaat
    30901 ataagtttag aagaaaatgt ttgggaaata aaaagagtaa gattagccaa tgatgcaaga
    30961 gtttcatata tgattactta tatgccagtt aagttatttc caaatttaaa taagactcat
    31021 tgtgaaaatt ctctttataa ttttgttgaa gaagtgtgtg gatacaagat agccatgtca
    31081 gagagagaag tacaagctgt aatatcgaat gaagagtgta tggataatct aaaattagaa
    31141 gaaccagagc cacttttata tataagccaa atttgtaagt tacaaaatag tgagattttt
    31201 gagtactcac acacatatca ctatggatat acattaactt tgaatgctgt tgtcgaataa
    31261 aatttaaata aaatgaaaaa gttttcttga aatgta
//
LOCUS       DAEORZ010000034        30674 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEORZ010000034 DAEORZ010000000
VERSION     DAEORZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.034"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(95..182)
                     /locus_tag="KRH39_001433"
     tRNA            complement(95..182)
                     /locus_tag="KRH39_001433"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(146..148),aa:Leu,seq:caa)
     gene            334..2982
                     /gene="polA"
                     /locus_tag="KRH39_001434"
     CDS             334..2982
                     /gene="polA"
                     /locus_tag="KRH39_001434"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBG8511350.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            2991..3593
                     /locus_tag="KRH39_001435"
     CDS             2991..3593
                     /locus_tag="KRH39_001435"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBG8511351.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            3586..4140
                     /locus_tag="KRH39_001436"
     CDS             3586..4140
                     /locus_tag="KRH39_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG8511352.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            4140..5855
                     /locus_tag="KRH39_001437"
     CDS             4140..5855
                     /locus_tag="KRH39_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8511353.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            5928..6140
                     /locus_tag="KRH39_001438"
     CDS             5928..6140
                     /locus_tag="KRH39_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBG8511354.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            6341..6940
                     /locus_tag="KRH39_001439"
     CDS             6341..6940
                     /locus_tag="KRH39_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511355.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            7168..7539
                     /locus_tag="KRH39_001440"
     CDS             7168..7539
                     /locus_tag="KRH39_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG8511356.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            7671..8945
                     /locus_tag="KRH39_001441"
     CDS             7671..8945
                     /locus_tag="KRH39_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG8511357.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGASSQNNSTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVTLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            9056..10000
                     /locus_tag="KRH39_001442"
     CDS             9056..10000
                     /locus_tag="KRH39_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511358.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            10216..11553
                     /gene="rsxC"
                     /locus_tag="KRH39_001443"
     CDS             10216..11553
                     /gene="rsxC"
                     /locus_tag="KRH39_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBG8511359.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            11576..12553
                     /locus_tag="KRH39_001444"
     CDS             11576..12553
                     /locus_tag="KRH39_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBG8511360.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            12553..13122
                     /locus_tag="KRH39_001445"
     CDS             12553..13122
                     /locus_tag="KRH39_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBG8511361.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            13135..13728
                     /locus_tag="KRH39_001446"
     CDS             13135..13728
                     /locus_tag="KRH39_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBG8511362.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            13743..14318
                     /gene="rsxA"
                     /locus_tag="KRH39_001447"
     CDS             13743..14318
                     /gene="rsxA"
                     /locus_tag="KRH39_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBG8511363.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            14334..15305
                     /locus_tag="KRH39_001448"
     CDS             14334..15305
                     /locus_tag="KRH39_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBG8511364.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDVTKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            15525..16109
                     /gene="maf"
                     /locus_tag="KRH39_001449"
     CDS             15525..16109
                     /gene="maf"
                     /locus_tag="KRH39_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBG8511365.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            16154..16813
                     /gene="radC"
                     /locus_tag="KRH39_001450"
     CDS             16154..16813
                     /gene="radC"
                     /locus_tag="KRH39_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG8511366.1"
                     /translation="MAPEERPREKMLVKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            16934..17944
                     /locus_tag="KRH39_001451"
     CDS             16934..17944
                     /locus_tag="KRH39_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG8511367.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            17954..18847
                     /gene="mreC"
                     /locus_tag="KRH39_001452"
     CDS             17954..18847
                     /gene="mreC"
                     /locus_tag="KRH39_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBG8511368.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            18862..19350
                     /gene="mreD"
                     /locus_tag="KRH39_001453"
     CDS             18862..19350
                     /gene="mreD"
                     /locus_tag="KRH39_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBG8511369.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            19362..22340
                     /locus_tag="KRH39_001454"
     CDS             19362..22340
                     /locus_tag="KRH39_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBG8511370.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYLAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            22438..23121
                     /locus_tag="KRH39_001455"
     CDS             22438..23121
                     /locus_tag="KRH39_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBG8511371.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            23145..23942
                     /gene="minD"
                     /locus_tag="KRH39_001456"
     CDS             23145..23942
                     /gene="minD"
                     /locus_tag="KRH39_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBG8511372.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            23960..24244
                     /gene="minE"
                     /locus_tag="KRH39_001457"
     CDS             23960..24244
                     /gene="minE"
                     /locus_tag="KRH39_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBG8511373.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            24329..25459
                     /gene="rodA"
                     /locus_tag="KRH39_001458"
     CDS             24329..25459
                     /gene="rodA"
                     /locus_tag="KRH39_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBG8511374.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            25500..25913
                     /locus_tag="KRH39_001459"
     CDS             25500..25913
                     /locus_tag="KRH39_001459"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBG8511375.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            26398..26727
                     /locus_tag="KRH39_001460"
     CDS             26398..26727
                     /locus_tag="KRH39_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8511376.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            26731..27522
                     /locus_tag="KRH39_001461"
     CDS             26731..27522
                     /locus_tag="KRH39_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511377.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            28087..29169
                     /locus_tag="KRH39_001462"
     CDS             28087..29169
                     /locus_tag="KRH39_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG8511378.1"
                     /translation="MKKIRKLATKLLIAMMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSEPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(29381..30574)
                     /locus_tag="KRH39_001463"
     CDS             complement(29381..30574)
                     /locus_tag="KRH39_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG8511379.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
BASE COUNT        12057 a         3455 c         5815 g         9347 t
ORIGIN      
        1 aaaagacaca gtaatttcaa aaaataactt aaaatttaaa tcaaaaataa aaaaactcct
       61 aatctattga ataaatagct ttaggagttt ttaatggtgc ccgaggccgg actcgaaccg
      121 gcacggagta ttagtccggt ggattttgag tccactgcgt ctaccaattc caccactcgg
      181 gcatgtcatg tatattatta tagcaaaaaa atatattgtt tgcaacatat ttaattttaa
      241 tatactatta ttattatggg aatattaaat aacagtatat tggtatataa tatatatgtt
      301 acaaaaatag ataattttta ggagtgatat aatttggaaa aaaaattaat tataatagat
      361 ggaaattcaa taataaatag agctttttat gctcttccag aaatgaataa taaagaaggg
      421 ctaaagacca atgcaatata tggatttaca acaatgctat ttaaaatgat agatatatat
      481 aaaccaacac atataagtgt agcatttgac agaaaagcac ctacatttag acatttagaa
      541 tataaagagt ataaggctgg aagaaaggga atgccagatg aactggcaga acaattacag
      601 ccattaaaag accttttaga taaatttaga ataaatagat tggaaataga tggctatgag
      661 gcagatgata ttataggtac tgtttctaaa aaagcagaag aaaatggata taaggtatac
      721 atagtaactg gagacaaaga tgctattcaa cttgcttcag acaatacaac tactcttata
      781 acaaaaaaag gagttggaga agttgaagaa tacaacttta attctgtaat tgaaaaatac
      841 gagatgactc caacacaatt tatcgattta aagggtctta tgggagacaa gtctgataat
      901 atacctggag tacctggaat aggtgaaaaa acaggtataa agcttataaa agagttttct
      961 agtatagaga atctaataga gaatacagat aagcttaaag gtagtgtaaa aaagaaaata
     1021 gaggaaaaca aagaaatagc tattcaaagt aaaagacttg caacaattat aagagatgtg
     1081 ccaatagaag tggatttaga tagtatgatt tttggcgatt atgataaaga tgaactttta
     1141 gataaattta gatactttgg atttacaagt ctcttatcaa gattagttga tttggtcgat
     1201 tcagatgaca cagaacacgt agaagaaaaa atagaaatat taaaattaaa agatatagaa
     1261 aaatttatag atgaagtaaa caaaaaagga caagttattt taaaaactgt tagaggacaa
     1321 ggtaatatac ttgaaaaaaa tataatatat atttttataa gtgtagatgg agaaaaaata
     1381 tattatgtag attcaaatga agtgtcaaag ttagatagta tattttcaaa taatgaaatt
     1441 aaaaagtatg gatataattt aaaagatgat tatatatctt taaaaccata taatataaac
     1501 ttagaaaata tgtattttga tataacaata gctgagtact taatagattc aacttcatct
     1561 acctcatatg attgtagtgc aatagctatg aagtatttat caaaaaaggt taaatcaaaa
     1621 gaagaattat taggaaaggg tgttaaatct aaaaactatg aggatttaga atttgaggat
     1681 ttagcagagt atatgggtca aataatatgc acagttaaag aaacagttcc tatgatggaa
     1741 agaagtctaa tggatatgag tatggatggt cttttttatc atgtagagat gcctttagta
     1801 gaagtcttag gtcatatgga gtatgaaggt ataaaagtag acaaaagtat attggatgaa
     1861 ttgagtgttg aatttaaaga aataatagct acattagaaa aggaaattta tgaattatca
     1921 ggagagccat tcaatataaa ttcaccaaaa caattgggtg ttatattatt tgaaaaatta
     1981 gagcttccaa taattaaaaa aactaaaact ggatattcaa ctaatgcaga tgttttagaa
     2041 aaattaagag ataagcatcc aataatagat aagataactg agtatagaca aatagtaaag
     2101 ttaaattcta cttacgttga aggactttta aatataataa atccagaaag taatagaata
     2161 cattcatcat ttaatcaaac tataacgact acaggaagaa tttcatctac agaaccaaat
     2221 cttcaaaata tacctataaa aatggaaatg ggtagaaaaa taagaaaagt gtttattgca
     2281 gatgataact gtaagttggt tgatgccgat tactcacaag tagagctaag agttcttgca
     2341 catatgagtc aggatgaaaa tatgatagaa gcatttaaac atcatgaaga tattcatacc
     2401 aaaacagctt cacaagtttt taatgttcct atggaagatg ttactagtga attgagaggt
     2461 gcagctaaag ctgtaaactt tggtattata tatggtaaaa gtgactttgg tctagctgat
     2521 gatttaaata tacctgttcc aaaggcgaaa gaatatatcg aaagttattt tgctaaatac
     2581 cataaaataa aagagtttat ggacacaaca gtagaaaagg ctagtgagga tggatatgct
     2641 gtaacaattt taaacagaag aagatatata ccagaaataa agtcaagcaa ttttatggaa
     2701 agaaatagag gaaagagatt tgcaatgaat gcaccaatac aaggaagtgc agcagatata
     2761 ataaaaattg ctatgataaa tgttcataaa aaattggaag agaacaattt gaaatcaaaa
     2821 cttatactac aagttcacga cgagttgata gttgaagcaa ttgatgatga aatagatata
     2881 gttaagaaaa tagtaaaaga agaaatggaa aatgctgtaa atatggatgt tcacttagat
     2941 gtagatttaa atgtaggtag ttcttggtat gagacaaagt aggtgatttt atgttgatat
     3001 taggacttac aggaggaata ggctgtggaa aaagcagcct atcaaatata tttagaaatc
     3061 ttaacattcc tatagtggat gctgatatta tatccaggaa gatatttgag gataagttgt
     3121 tattggaaaa agtatttgtc cattttgggc aaagtataaa aaatgatgat ggaactctaa
     3181 atagaaaagc tcttgggaaa atagttttta gtgatgaaga aaaacttaag gaattaaata
     3241 acttgactca tccacgaata agagaaaaaa taataagtga aatagaaaaa ttgagaaaaa
     3301 aaggcgaaaa tatagtagtg ttagatgcgg caatattggt agaaagtggt tttttagata
     3361 tggttgacaa gctattagta gttacttgta aacaagaagt acaaattagt agaatacaaa
     3421 agagagataa ttgcagtgag caggaagcac ttagtaggat aaattcacag atgagtcagg
     3481 aagaaaaatc caaatatggt gattatataa tagataattc gggaacaata actgaattag
     3541 aaagtaaagc acataaattt attgagtaca tgaaggagaa ttggcgtgaa tagtaagaaa
     3601 gtattgattc tatctatttt tataatctta tttggggcac tattaatgga aagcaaagta
     3661 atacataaat ttttatatcc taaaaaatat tcagagtatg tagaaaagta ttcgaaagaa
     3721 tttaatttag atgaaaatat agtttacagt gttattaaag ccgaaagtaa gtttaatagt
     3781 tctgctgttt caaaaaagga agcaaaagga ttaatgcaaa tattagacat aactagagat
     3841 tggggagcag aggaactaaa tttaaaaaat gtggatattt tcgacccaga gactaatata
     3901 agacttggct gttggtattt aagtaagtta tacaaagaat ttggtaaatt agatttagtg
     3961 atagctgcat ataatggtgg ttcaggtaat gtgaaaaaat ggttagaaaa taatgaatat
     4021 agtaaagatg gcgaaaatct acatgatata ccttttaagc aaacttcaaa atatgtagaa
     4081 aaagtaaaaa ataattacga acattataat aagatatatg gcaagaaagg aaaaaactaa
     4141 tgaagagaat aaaagtctta acagtcgttt tagctataac tcttatggta gctggttgta
     4201 gtaatactaa aacaaaacaa agtagtagtg attcaagttt acaaagtcaa agttctatag
     4261 atgctaagta tataaactta acaatggtaa ctccaaaaac tataaatcct ataactaata
     4321 ctgataagtc tgttggttat ataatgaatt tagtttatga tagtttattt actatagatg
     4381 agaactacaa tgtaattcct caattagtta aggagtataa tatagctcag gatggaatga
     4441 gtatagatat aaagctaaaa gatgcaaagt ggcatgatgg aaaaaatgtt acttcaaatg
     4501 atgtaaacta tactattggt ttaattcaaa aaagtgcaga tagtccatat aatgaattta
     4561 ctaaaaatat agcttctgtg aatattaaat cagataaaga ttttacaata aaatttaaag
     4621 ctagatatgc tttttcaatt gatagtttaa tttttccaat tgtatctcaa aatcatttag
     4681 attcaaaaga tgtaaatgac aagaataata atatgatagg aaatgggaaa tataaaatag
     4741 aatcatatac agaaagagaa ggaatggttt tatctgtaaa caaagattac tatgaagaag
     4801 ttccaaaaac catgaagaac ataaaagtag gtatggtgcc taatgaagat gcaaggacat
     4861 ctatggttat ggctcttgat agtgatataa ccaatgttac attaaatgat ttaagtaagt
     4921 ttcaagaaaa agagtttaat ataacaaaat atcaaggaag agattatgag tgtgtattat
     4981 ttaattataa taatcctttt ttcaaagatg ttaattttag aaaagcaatt gcacactcta
     5041 tagataaaga tagaattatt agtgaaggtt atatggatga tgctactcca gttaattttc
     5101 cacttaattc aaaatcaaaa tactataata gtgaaatgaa ggatttagag tttaataaag
     5161 ataaggcgat agagtgtctt gctaaggtag aatatgcaaa tgtaaactca gtaaatcaaa
     5221 atgacaataa agtaaaaaat cagaaaaaat cagctaaaaa gaactcaaag aagcttacgc
     5281 cagaagaaga agcaaaagct aaaaaagcag aagaagatag aatcaaaaaa gaagctgaag
     5341 agaaaaaaaa tagagaaaaa gaggaagtaa agaaaagtct ttctgaaatg aacttaaaga
     5401 taatagtaaa cagagataat agtgagagaa taaaaactgc aaacataata aatgaaaact
     5461 taaaagctat aggaataaat agtacagtaa atcagctgtc agataaggat atggaaaatg
     5521 ctcttaattc taaaaattat gatttagcat tagttggatg gaaactatca attataccag
     5581 atgcaagtag tattatagcg agtagtggat atactgatga taagttaaat ggatatatgt
     5641 ctgcacttac aagttctacg tctgaaattg aaacaaaaaa agcatacaaa gatgtacaga
     5701 catatataaa agataatgca acatttataa gtttagctgt gagaaacaac tatattgtaa
     5761 gtaatagtag attaaaaggt aaaattactc caaatgattt tgatgtatat gagggaatat
     5821 ctaatttaga tataaaatcg aatgaatcaa attaaatgat taatttctat ttaaaaagta
     5881 taaactatat gatagaatac ccttataggg tataaggagg taattaaatg aagaagaaac
     5941 tattagtaga aggtatgagt tgtggtcatt gtgtaaacca tcttaaaact gcattaacag
     6001 aagatataga tggtattgaa gttttagatg tagatttaga aaataagtgt gcatctgtag
     6061 atatgaaaga tgatgtatct attgaaaaat taaaagaagt gatagcagaa ttaggatttg
     6121 aactaaaggg aattgaatag aaattgataa actaaaaaaa gacctctatt gggggagtaa
     6181 tagaggtcta aaaatggggg agattgagta tgtttgattt tacattacta ttatctcccc
     6241 ttttaaatta atatatacat aaaaagtaaa aaaattttgt aatgactcac atttcttgag
     6301 ctttttgaat atactataaa tagctaaggg agggagtatc atggaatcta ggggatataa
     6361 agaggattta aaaaaaatga tacttgatat ggcccacaaa gaacttgaga gttatgtatc
     6421 acaaaaagga gcaaaaaaat actttgaagg taacttggat aagattatta ataataatat
     6481 aaaaaaattc aatcaaagta ttggtcttaa tagagtatat ttaaaaatgt taaaaaagtg
     6541 tgcatatcaa gaaagtgtaa cttcaatatc aaggactata aagaaagaag cattatttaa
     6601 atcaaaaaaa gagctatttg ctttttcaga gtacttaaaa cttgatatta acaagcgact
     6661 ttcatataat caaattttaa gaaaaatatc aagatatata tactacaata gaagttcata
     6721 tgctcaaaaa tacgttgttt tcaaaagagg agatgaagaa tatcttttag aaccagagaa
     6781 aataaaagat gaattaatat caagctatag gtcaaagact agagaagata tgaggtcaat
     6841 agcaaaactt ttggatatag aagtagaaga tgattataat gctgaagata ttagaaaaaa
     6901 agtaataaat tatattatta aagaaaaact taaaagttag agttttaaag aataagttct
     6961 gccaatttgc atgattaagt ataacttggg tataaatttt ttatgcattt gaaagagctt
     7021 attctttttt tgacatattc ttttaaataa tttaccatga tttggtaatt ggtcatcatc
     7081 attcaaattt ttaacttaat atgatttaaa taaatttata ataatgatat aatataaaaa
     7141 aaataaaaat tggagagtga tttcaaaatg aagtttaatt tttgtcataa caatttcaat
     7201 gttactaatt tagaaaaaag tttagatttt tataaaaaag ctttagggct taaagaggta
     7261 aagcgcaagg aagcagaaga tggaagtttt atattagttt atttaggaga tggcattact
     7321 agtcatacat tagaattaac ttggcttaga gattgggata gaccatataa tttaggagac
     7381 aatgaatttc atctggcttt agaagttgag gagtttgatg aggcaaaaaa acttcataaa
     7441 gatttagatt gcatatgttt tgaaaatgaa agtatgggaa tttattttat agcggaccca
     7501 gataattatt ggattgaaat acttcctaaa aatcactaat tttaaggata tgtttataat
     7561 ttctttacaa ttaataagaa aataagttat cattaaattg tagggactaa aaatggataa
     7621 taatgttatg aatttatgac tataaaaatt tgttgggggg agtgatagta gtgaaaaaag
     7681 ctatagcagc tttaggaatt ggggcagtag cagtatctgt tagctccata aatgcaagtg
     7741 ctcttgaaaa gggaacagta acagcaagtg ccttgaatat aagaagtgga ccaagttctg
     7801 attgcgataa ggtagcaaaa ttatacaagg gaaaaactgt agagatttta gaaaagtcta
     7861 atggatggta taaagtaagg gtatcgagtt ctgttgttgg atggggaagt gcaaaataca
     7921 tatcaacaag tggttcgtct gaaggtgcat caagtcaaaa taattcaact tcaagtggga
     7981 caactattag tggaaatggt aaagtaaatg tgagttctag actgaatgta agaagtggtg
     8041 caggtactaa ctattcatta gttggtaaag caaataatgg agatgtagta acgttactag
     8101 aacaaagcaa tggatggtat aaaataaaac tatcaaatgg agttactgga tgggctagta
     8161 gccaatatat ctctaaaaca tcagaggatg ttggaacgaa taattctagt aattcaaata
     8221 gtactaataa ttctgataaa aaaccatcta gcgaagaatc aattgaaggc aagaatggta
     8281 aggtaacatc tgctgtgagt ttaaatgtaa gaagtggtcc tgggacaagc tattctataa
     8341 taggaaaatt aaatggtgga gatgttgttg aattaaaagc taaaagtaat ggttggtata
     8401 aggtaaagtt atcgagtgga acaattggat gggtgagtgc cagttatatt tctgagacta
     8461 atgaggatac aaaagaaaaa ccaaattcat catctaacca aaattctcaa tctaatagta
     8521 actcaaagcc atcatttact ggaaattcag ataaatctac agcaaaaggt tcaacaatcg
     8581 tagattttgc atatactctt attggaatac catatcaatg gggagcttca ggacctgata
     8641 aatttgactg ttctggattt acacagtatg tttttaaaca ttctgtaggc gtttctattc
     8701 ctagagtttc tagagaacag gctaattttg gaagtgcaat ttctatggga aattatgcac
     8761 caggagattt agtttatttt gacactgatg gtgatggaac aactaatcat gtaggtatat
     8821 atgtaggaaa tagtaaattt atccattgta gtggtactca gactaatcct aataaggtta
     8881 aagtggataa tttaactagt tcatattggt ctaaagtatt acttggtgca agaagatttg
     8941 tttagtaagt acaatttctt attaaaaaat tagaagaaat aattatttat attcataaat
     9001 tgtgactctt aaaaatatac tttattaata atataatttt gggagggaca aactaatgaa
     9061 tagaagaaaa acgatataca gaggatttaa taataataga cataagattg atttacgtaa
     9121 atatgttata acgattgctt gcttgtgtct aattggctat tattcttata caaaaataaa
     9181 agatagtaag atattggagt atgtatcggc taaaatacca tttctaaata attcatcaga
     9241 tataacttat aaagatatat ctgatgaatt aaattcaata aaaaaaggga aaaaatctaa
     9301 atcacaaacc aattcagatg ataaacaaga aactaatcca gaaaaagcgg taaataacac
     9361 taaagagcca gaagaagtca agttagctac tatagaaggc tgggatatgt acactattca
     9421 agttgctgca atagataata atgatgattt gaaaaaaata cagacatcat tagttaataa
     9481 tgacatacct ttttcagtta tggagaagga tggagtaaaa aaaatacaaa cctattcctc
     9541 ttttgatgaa aatgatgtta gaaagcagat aagcagtgta agaaaggtgt ttccagatgc
     9601 atttttatca cacttagatg cacctatgtt gtctttagag tatacaagta actacgctta
     9661 tatagagagt atatctaaag aattaaataa attaataaca aactttaaag aagaatcaag
     9721 tttttggtcg aatgctgaaa ataatgtaga tatggaaaaa tataatacta ttttgacaaa
     9781 tagaaaggca atatcacaaa atatatcaaa agaagctgaa aagattgact attctgaaat
     9841 gcgtttattt aaagataatt taatagagta tgtaaagaat gtgaatgaaa aaattgatac
     9901 agcttcaaaa gcagcaaatg aagaaaaata tagtgtaagt aaaagtctgc ttttatcatc
     9961 catgcaggag tactctatgt ttataaattc tataaaataa aatacagaaa atttggactt
    10021 tagaaagcta tatgaaaagg attgtttaat taagcaatcc ttttcctcgg tttgacttaa
    10081 tatagaggta ttgatttata aaataaatta taaaaatacg gatatttttt taacaattgt
    10141 taagattttt taatatatat agtatactta tagaaggcag aaaaaatgtt tttttataga
    10201 taagggggta aaacaatgaa actcttaact tttaagggag ggatacatcc tccatataga
    10261 aaagagtact ctaacacaaa ggcacttgaa aaagctcaag ctccaaagat agtttacatt
    10321 cctcttcagc aacatatagg ggcgccagct aaacctattg ttgaagttgg tgatgaagta
    10381 aagtttgggc aaaaaatagg tgaacaacaa ggttttgttt catgtaacgt tcattcttca
    10441 gtatcaggta aagttattgc tattgaacaa catgaagtac caggaggttc agctcaatgt
    10501 gtagttatcg aaaatgactt taaggaagaa ttacatgaga gtgttcaacc aaaaggtcag
    10561 ttagaggatt tgagcaaaga agatatagta ggtatcataa aagaggcagg aatagtaggt
    10621 atgggtggag ctacattccc taaccatgta aaagtatctc ctccaccaga tagcaaggca
    10681 gaagtagtta tattaaatgg tgcagaatgt gaaccatact taactgcaga ccacagatta
    10741 atggttgaaa atccagaaga tgttgtgttt ggtttaagag cattaatgaa ggtattagat
    10801 gtcaaaaaag gatttatagg aattgaaacg aataaaccag atgctataga agcaattcaa
    10861 aatgtagcta aagactatag cgaaatcgaa gttgttggcc ttcaagtgaa atatccacaa
    10921 ggagcagaga aacaacttat ttatgcgtgt acaggcaaag aagttccatc tggtggatta
    10981 ccaatagcag caggtgcagt tgttgacaac gttgccacag cagcacagat agctaagtct
    11041 attaaaacag gtatgccttt ggttgaaaga atcactacaa taacaggtag ttgcatcaaa
    11101 gagccaaaga acctaataac aaaagttgga acattagttt cagaaataat agaacaatgt
    11161 ggtggattta aagaagataa gaagattggt aaggttataa tgggtggacc tatgatggga
    11221 atagctcaat atactactga aatagctact aacaaaggtt cttcaggaat tttatgtttg
    11281 gatgaggaag aatcacgtac tccagatatt caaaactgtt taagatgtgg aagatgtaca
    11341 gatgtatgtc catcattctt acaaccactt ttcataagtg catattcttt aaaagatgac
    11401 tatgacacag ctgaatatca tagagcaatg gattgtatag aatgtgggtc ttgctcattt
    11461 atttgtccag caagaagacc attacttcaa tctataagat cagcaaagag agaaatagga
    11521 gcaaagagaa gaaagcaagc tgctcagaaa taaagaaagt taagggggaa aaacgatgga
    11581 aaataagttg atagtatcat cttctcctca tgtgagaagt aatgaagata cttcatatat
    11641 aatgaaacaa gttattatag cactccttcc agcagcagta gcaggagtat acttctttag
    11701 acttaatgct ttgagtgcta tgtttttttg tatacttggt acagtaggta ctgaattttt
    11761 atatcaaaaa tttacgaagc aaaaaagtac tataggagat ttttcagctg ttgtaacagg
    11821 attattatta gcatttaacg taccagcatc acttccttgg tggatgtgtc tagtaggagg
    11881 aatatttgca atattagtag ttaaaatggt atttggtgga attggatgta actttgtcaa
    11941 tccagcactt gctgcaagag catttttatt agcatcattc ccagtagcaa tgactgcttg
    12001 gactcaacca ggtgttaact ggataggtaa aaatttagat gcagttacta cagcaactcc
    12061 attaagcttt ttgaaaaatg gagcagcagg actggcagac ctttctagca atggaattag
    12121 tcttgctgac atgatgatag ggaatattgg tggatgtata ggtgagacat cagcaatatt
    12181 attattatta ggtggagtat acctaatgta taaaggcata ataaattatg ttataccagt
    12241 attttacata gcaactgtat ttatattaac attcctttta ggtggattta atataacttt
    12301 cgcaatatat caactgtttg ctggaggact tatgttaggt ggattcttca tgcttacgga
    12361 ttatacaact tcgcctatga ctaaaaaagg tcaaataata tacgctgtat tagctggtct
    12421 tataacaact gttataagaa tgtatggtgg atatccagaa ggtgtatctt actcaatact
    12481 attagtgaac tgtcttgctc cacttataga taagtttgtt agaaatagag tatttgggga
    12541 ggtggctaaa taatgaacag tatggtgaga ttaggtggta ctttacttgc tataagtgca
    12601 atagctgcat tagctcttgg agctactaat caggttacag ctccagtaat agaacaaaga
    12661 aatatacaag caaataatga acttagaaaa gcagtccttc cagaagcaaa agaatttaaa
    12721 gaaatgaagg aagatactta caaaggcctt ggagatggtc ttatagctga agtgtatgaa
    12781 ggtttagatg gttctgaagt ggttggatat actttaaaag ctaaaccaag tggatatggt
    12841 ggagaaattg aagttatggt aggtatatca tctgaaggtc aagttacagg tgttgacatt
    12901 ggtaatatgt cagaaacagc aggtcttgga gcaaaagcta aggatgatgc atttaagggt
    12961 caatacaaag gaaaaactgc tgagcctctt gaagtagcaa aaggaagtac aacagcagat
    13021 aatcaaatat tagcaatatc aggtgcaact attacatcta cagcagttac tacaggagta
    13081 aatgctgcta tagatgtttt caatagtgct cttaataaat aaggagggtt aattatgaat
    13141 ttagctaaag tatttaaaaa tgggctaata gatgagaacc caacatttgt tcaagttata
    13201 ggtatgtgtc caacactagc cgttacaact tcagcaataa atggaattgg tatgggtctt
    13261 tcaacagcag cagttttaat atgtgcaaat ttagttatat ctttaataag aaaaattaca
    13321 ccagataaga taagaatacc tatatttata gtagttatag ctacatttgt tacaatagta
    13381 ggtatgcttt taaaagcata tgttcctgct cttgataagg ctcttggtat atatatacca
    13441 ttaatagttg ttaactgttt aatattagct cgtgcagaaa gttttgcttt caaaacagga
    13501 gctatgcctt caatagttga tggtgtaggt caaggtcttg gatttactgt tgcacttaca
    13561 ataataggtg cagtaagaga attacttgga aatggtagtt tatttggaat gacattattt
    13621 ggagcatctt tccaaccagt attaatattt atattaccac caggtgcatt cttaacatta
    13681 ggattcttat ttgcaggatt taataaatta agaagtaaaa aagcatagtg gaggtgtaat
    13741 aaatgaattt aatactttta tttttaagta tcgttcttgt taataacgtt ataacatctc
    13801 aattcttggg tatatgtcct ttcttgggag tttctaagaa ggttgataca gcagtaggga
    13861 tgggagttgc agttacgttc gttttaactc tagcttcaat tataacttat tttatacaga
    13921 ttttattagt aaaaactgga actggattct tacaaacaat agcttttata ttggttatag
    13981 cttctatagt tcagttcgtt gaaatggtaa ttcaaaagat gagtccatct ttatatcaag
    14041 cattaggagt ttatttacct cttataacta caaactgtgc agttcttggt atagctctag
    14101 ttaatgttca aaaaagttat aatttagtag aaacaataat aaacggattt ggagccggag
    14161 ctggatttac tttagcaata gtaatattcg caggtataag agaaagacta gaattagctg
    14221 atataccaga agcatttaaa ggtttcccta taacgcttat atcagcaggt ttaatgtcaa
    14281 tagctttctt aggatttaca ggacttataa agctataaaa aagaggggtg aaaatggtga
    14341 tacttacagc tgtattagtt ttaggaatta tgggcttaat cttcgggata gtactagatt
    14401 ttgcatctaa aaagtttgct gtagaagtag acgagagagt ggaagctata ctaggagtac
    14461 ttccaggagc caactgtgga ggttgtggat ttccaggttg tggaggtctt gcaaatgcga
    14521 tagttgaagg caatgcacca gttaatggtt gccctgtagg tggagcagat gttggtgcaa
    14581 aagttggaga aataatggga ataagtgctg aagcaggtga gaaacaagta gcaaaagtta
    14641 tttgtaaagg aacttgttca agtgcaaaag ataaatatga gtatgaagga atatcagatt
    14701 gtagagcagc aaatgtttta aactcaggtg ctaagatgtg taaatttgga tgtttaggtc
    14761 ttggaacatg taaagatgct tgtaaatttg atgctatttc tatagttgat ggaatagcag
    14821 taatagatga agaaaagtgt gtaaattgtg gaaaatgtaa agaagtttgt cctaagggta
    14881 taataatcac aaaaccagaa agtcaagaag tagttgtaga gtgtaatagc aaagagtttg
    14941 gtaaagctgt taaggaaaaa tgtactgctg gatgtattgg ctgtggaatg tgtgtaaaag
    15001 cttgtaaatt tgatgcaata atatttgaag acaaaatagc taagatagac cctaataagt
    15061 gtgtaggttg tatgcaatgt gtagcaaaat gccctacaaa agtaatatca ggtgacgtaa
    15121 ctaagaaaaa gaaagttact attgaccaag aactttgtgt tggatgtaca gtgtgcaaaa
    15181 aacaatgtaa gtttgatgca atagagggag aattaaaaga aaaacataag gtagatgcag
    15241 ataaatgtgt aggatgccat ttatgtatgg aaaaatgtcc aaagaaagct attaaaattt
    15301 tgtaatatgg acaaaataag gatatggaat aatttccata tctctaatga gtacataaaa
    15361 cagaggtatg gaataatttc catacctcta ttttatgtat atttattcta atggtaataa
    15421 atataacaat agtgtaacaa aaaaggttcg acatttctgt tgaatttttt acaatatagt
    15481 gatagactaa ataggtacaa attacgtaca aatgaggtga ataaatgaat ataatcttgg
    15541 catcagcctc tccaagaagg aaagaaattc tagagaatac gaatgtaaga tttgatataa
    15601 ttaaaaatga aattgatgag attatattgg aaggtgaaac accaaaacat ttagttatga
    15661 gattagcttt tgaaaaaagt atgtctgtag catctgaaca taatgaagat gttgttattg
    15721 gagctgatac agttgttgtt ttagacaatg ctattttagg aaaaccaaaa gatgagtctt
    15781 gtgcaagaga tatgttaaaa agactatctg gaagagagca tcaagtcata acaggtatta
    15841 gcttaataaa cttgtgcgaa gataaaaagg taattgacta tgtaatcagc aatgtgaaat
    15901 ttaaaacgtt atcagagcag gatattgagg attatctgaa aactaatgag tcatttgaca
    15961 aagcaggagc ctatggtata caaggttatg gagctttgtt agttgaagaa attagaggag
    16021 attattttaa tatagttggt cttccaattt caaggttagg cgacctatta aaaaaatatt
    16081 ttagtataaa tcttttttat ggagtgtgat ttatctgaaa aagtcattta attcaaccat
    16141 taaggtaaaa gaaatggcac cagaggaaag acccagagaa aagatgcttg tcaaaggcgt
    16201 aaaaagctta tcaaatgctg agttgttggc tatactttta agaactggca acaagaataa
    16261 aaatgctatt gaattagcca actatataat aaatagagat attcagggta ttagacattt
    16321 agaagatatg acaatagagg agctgtgtaa tatagatgga attgggttat caaaatcaac
    16381 ccaaattaaa gcagctttag aactaggctc tagagtagct agttttaaac ctataaagta
    16441 taaaataagg aatccttggg atatacagag gtattatatg gatagcctaa ggtatttaaa
    16501 aaaagaggtt tttaaagcgg ttcttttaaa cacaaaaaat gagataatat ctgatgtaga
    16561 tgtatctata gggactttaa gctcgtcatt agtccatcca agagaggttt ttaaagaagc
    16621 tataagaaga agtgcaagta agataatagt gatgcataat cacccttcag gaagtgtaga
    16681 accatcaaga gaagataaaa atatcacatc aagacttatt aaatgtggag aaataattgg
    16741 aatagaaata atagaccata taattattgg agatggatta tactttagct ttaaagaaaa
    16801 tatgataatt tgactttata tgtgataaaa taataggaga atgttttaaa catgattctt
    16861 aatagtagga aggagtaaaa tcatggctaa ggaaaaaaag aaagaaaaaa aaggtttttt
    16921 tagttttaat aagatgacaa aggatatggg aatagattta gggactgcaa atacattagt
    16981 ttacataaaa ggacaaggta ttgttgttag agaaccatca gttgtcgcaa taagagatga
    17041 tagtaaagag gtattagcag taggtgaaga agccaaaaaa atgataggta gaacaccagg
    17101 aaatatagta gctataagac ctatgaaaga tggagtaata gctgattttg acataactca
    17161 atcaatgatt agctatttta tacaaaaagc ggctgataaa aaaggtgttg taagtccaag
    17221 aatagctata tgtgttcctt ttggagttac agaagtagaa aagagagcta tagaagaggc
    17281 tgcaagacaa gcaggagcta aagatgcatt ccttatagaa gagccaatgg cagcggctat
    17341 aggggctgga ttaaaagttg aagaaccaga aggtaatatg gtagttgata ttggtggagg
    17401 tacttcagaa atagctgtaa tatctttagg aggaatcgtt actgctaaat ctataagaat
    17461 aggtggagac gaatttgacg agtctatagt ggcttatgta aaaaaagaat ataatcttat
    17521 gataggtgaa agaactgctg aaaatgtaaa aattaacata ggttctacat ttaaagatga
    17581 tgaagaaata aatatgcaaa taagaggtag agatttaatc tcaggattac ctaaaacaat
    17641 agaaatatgc tctacagaag ttagagaagc attaaaagaa cctgtaagtt caatagtaga
    17701 tgcaataaaa tctacactag aaagaactcc accagaatta gcctcagata taatggaaaa
    17761 cggaataatg ctaacaggag gaggcgcttt gcttagaggt cttgataagc ttattacaca
    17821 ggaaactggt atgtctgtac aaatagcaga aacacctctt gattgtgttg cacttggaac
    17881 aggtaaatct gttgaagacc aagaaatctt tgaaaaagta ttaatgatga atactaagaa
    17941 ttagaagttg gtgatggcct tgagatttga caagaacaaa aatgagaaaa aaaggattaa
    18001 tgttaaagtg atagcaacgg gcgtagttgc tatcacttta attggaattg tgggtatatc
    18061 aattggtaaa ttttctagtg gtagtccagt aaatttagga gtagcttcag atgctattac
    18121 aagtgtaggt aagggaataa atgatggatt ttcttttatc aaaaatggtt ttaaagatgt
    18181 agcaaatttt aaagataatt ctaaaaaggt aaaaaaatta gaagaagaaa atgaaaaact
    18241 taaaaaaaat atgatagctt taaatgccaa actagataaa acagaatcgc tagaagagtt
    18301 aaaaaagaca ctgaactttg tacaagaaga gtataaggcg acaagtattt cagcaagtgt
    18361 tgttggtaaa aatgatggaa actggtatga gagttttgtc ataggtgcag gaaaaaattc
    18421 aggtgtaaaa aaagaaagta tagttatgaa tggtagtgga ttagttggca tagtttatga
    18481 ggtttccaac aattatagta aggcaatttc actattggat tctaaggcat ctgtaagctt
    18541 taaactagca aaggatgcca atgctaaagg tacaattact caaaatacta ctttagataa
    18601 taaagatagt tataatagta aaggatattt gcaaggatat atgtttgact catcatataa
    18661 tgtaattcaa ggagatataa taacaacatc aggtcttgga ttcttcccag atggtatacc
    18721 aattggcgaa gttgaaaaag tggttgatga taaagataag tcattaaaat atgtagttgt
    18781 aaaaccttat gttgatttta agaatataaa tgacgttgta gtcatagaac caagaaatat
    18841 aggataaagg ggataaaaac aatgaaaaaa gttttacttt gtctgttggg gattgtgctt
    18901 atgactatag aaaatagtat aacaaattat atagatattt ttggcataag ctttaattta
    18961 gttcttatat atgtaaccat aatctctctt tatttagatg aattagaagg tgggattata
    19021 gcagcaataa tagggcttat taaagacata accgttggtg gaatttttgg tgtaaatgca
    19081 ttaattttgt ttattattgc atatgcaatt ggatacatga gagatcagct atataaaggt
    19141 agttatatta ttactttcgt tttaattttt ataggcagtt taattgattc aattgtcaat
    19201 ataggtacat ctagtataat ttatcaaagc tacaacatat caacactttt tgtaaaaggt
    19261 ctactagtag caccaatagt gaatagttta attggtttag taatatatcg cttatctaaa
    19321 agagcagttt taaaacttaa agaagattag ttggtgattt catggaagaa aataaacaag
    19381 ttgatagact aaaaataatt aagatagtca tgggcgtgat atttttagct attttagtaa
    19441 aaataatcta tatgactaca tttaaatatg aatattacaa tgagttagct gagaataaaa
    19501 catataaaaa gttggcaata gaagctccaa gaggagaaat aaaagataga tatggaagac
    19561 tgttggcagg aaataaaaac ttatttactg ttcaagtttc aggtaacgat attaataaga
    19621 aagatgcaaa taagcattca agagcaaatg aaatatcatt aaaacttata aatttgctgg
    19681 agagaaatgg tgaagaatat gtagatgagt ttcctatata tgtagaaaat ggaaagtact
    19741 attatacata tgacagagat attagggaat ataaatcaga aaatggtatt ccaaatgatt
    19801 ataatgctaa ggaaagtttt tattacttgg tggacaaact tatatcagct ggaatattat
    19861 ctcaagaaga taaaagattg gatgcgacaa gattacaggc aaaactaaat gaaaatggat
    19921 attatccacc tattttagtt agtaaatgga tgttcacagc agaaagagat aagagagatt
    19981 ggttggctag ttataagata aaagaaacta aactgagtgc caaagaagcg tttgaaaaag
    20041 taagaaatag tgatgcatta gaaattgata aaagtttgag tgatgaagat gcaagaaaaa
    20101 taatggtcgt aagggatttg ataaaatcaa aaggatattc tcaatataat ccagtaacta
    20161 ttgctaagga tgtaggagaa actacgattg ctcaaattga agaaagtgct atggaccttg
    20221 ttggtgtttc aatagcagta gaaccagtaa gatattatcc taatgggtca ttagcttctc
    20281 atatgttagg atatgtagga aaaatgccat ccactcaaat agaatcatac cttcaaaaag
    20341 gttatgaaac tggagacatg gtaggtcttg caggagtaga aaagagtaat gaaagtagat
    20401 taagaggaac tgatggttat aaaatggtta aagttgatgc attgggtaga atcagtaaag
    20461 aaattgaatc taaaaaacct aaatcaggag atactgtgta tcttacacta gataaagatt
    20521 tacaagaagt ttcagacaat gcattaaaac agataatcga agttgcttct aaaggaggta
    20581 cttttaaaag taaatttggg gacaaaccaa ttagtgcata tgcaggaaaa gcacagtcag
    20641 cagctcttat agctattgat gttaaaaatg gagaagtatt agcttcatca agttatccta
    20701 attatgaccc aaataaattt gcaaaaggta tttctacaga ggattataaa gcacttcaac
    20761 caaaaaatcc aaatgactta ctagcaggaa gtccactttt aaacttggtt acacaagggg
    20821 aatttcagcc aggttcttca tttaagatgg taacttcaat ggcagcgtta gaaaatggtc
    20881 tggatccaaa ctttactata aatgaccctg gggttataat gttaggtaaa aaatcttttg
    20941 gtgactatgt atggaaccat ggtagaggaa accatggtat gacaaactta tataaagcaa
    21001 tacaagagtc ttgtaatata tatatggcaa caattggtac aggtaaaaca tggccagatg
    21061 gaaaaagcat tggtatagat atgaatgcaa ataaaatact tgagtatgca aaattatttg
    21121 ggcttgacca aaatacaggg cttcaagatg aagtagaaga aagagcaggt aaagttccta
    21181 gcacagaaga taaattgaaa tcaacacaag ctcttctaaa gtcaaattta gaaagagaaa
    21241 tggcaaatga ttttgttgat ataactagag aaaaaaatcc taaagaatat gagaaaagaa
    21301 tcaatgagat tgtaagctgg gcagcagaaa agaaaactcc tggtagagtt gaaacaatga
    21361 ataggctaaa aaaattggat gttaaagaag atagaattga agaagtagca gatttagcag
    21421 tatttagtta ttttaacttt gcaaaatgga gtactgcaga tacatttaac ttagctatag
    21481 gtcaaggtga aaatgcttat acaccagctc aaatatcaag atatttagct gctatagcaa
    21541 atggaggtaa tttagtagaa ttatctgtag ttgatagagc tgtttcaagt gattattcat
    21601 cagttaaaat taatgaccag aagaaagtcg aaaaaatacc ttttaaaaat ccagataact
    21661 taaaagaatt aacaaaaggt atgaaattgg ttgcaagaca aggtacagcg aagagtgctt
    21721 ttgctgattt tccaatagat gtagcagcaa aaactggtac agctgagaaa agtgggaaaa
    21781 tacctacaga taacgagtat gaatatttaa aaagtcatat gtcatcttat aatgtcaacc
    21841 taaatgatgc gattaaacta gctgataaga tgaaagcaga aaaagaaaaa gagctttcat
    21901 tagctaagga aaaggaaata aaaaagaaat tagaaaataa agatttgaaa gacgaagaaa
    21961 gaaagaaatt agaggaagaa ttagaagatg gagtaaaagt tagattagag gacactgata
    22021 aggtgaattc ttcatatcta agaaaagcta taaaagaatt aaatccaaaa ataacagatg
    22081 accaaataga tagatttaaa caagattatg gttcttttac gtggactgtt gcttttgctc
    22141 cagcagatga cccagaaata gctgttgtat gtgtaattcc tcaaggtgat tcaagtgtat
    22201 tctcactttt accaactaga gaagttattg gaacttatat gggattagag ccaactaata
    22261 gtaaaaatga caacaagacg aatgatgtta acaattcttc tgacgaaaat ataaatttcg
    22321 agtcacaaat taatagatag ctcaatctat gtataaactt tagctttata tagaaaaaat
    22381 aaaagggttt aaagcgtatt tgaagaatat aaatgatata tcactctgga ggttgttatg
    22441 tctttaagag aaatttgtag tcaagaacta gtagagttca agggtaataa aagagggata
    22501 atagtaaata taaaaagaga agctccattt gaggaaatac aggaaaaaat aataaacaaa
    22561 ttggaagcat atgttggctt ttttaatggg gctaaaatta gtaaaattaa tagtgattgt
    22621 ttaactgaca tggagattct agaacttaaa gaaggaatta cttctagatt tgatgtggaa
    22681 tttgttgaag atcaaaaaat tgaagaaaat agtaattttc caacaaaata tgtcaatacc
    22741 ctaagatctg gtgaaaatat tgaatttgaa ggtgatgtag ttatattgaa tgatatgaaa
    22801 cctggttcaa aagtcctttc aaaatcaaat acagttgtta tgggagatat aaatgctggt
    22861 gctaaggttg tagcaggagg taatgttttt gtaatgggta aaatagaagg ttttgtacat
    22921 gcaggtgcag aaggcaatga atttgcatat gttgttgctg gaaatcttaa tcctaaaata
    22981 ttacaaatag cagataatat tgcagaagct ccagatgatg aagaaaatta tgaaagtgaa
    23041 tcagaaatta gcccagaaat agcctttgta agtaatggaa gaattgtaat cgaaagctat
    23101 ttgtcaaagt tagacaaata gaaagaaaaa tagggggtag tattatgagc gaagttatag
    23161 ttataacatc tggtaaaggt ggggttggaa aaactactac cgcagcaaac ttaggaactg
    23221 cgctaagcct agaaaataaa aaaacagtag ttgtggatgc agatattgga cttagaaact
    23281 tagatgtagt aatgggtctt gaaaatcgaa tagtttatga catagtagat gtagttgaag
    23341 gaacttgtag gcttaagcaa gctctaataa aagacaaaag atttgataat ctatatttat
    23401 taccagcagc acaaactaga gataaaaatg ctgtctcagt tgagcagatg attgacctgt
    23461 gtgagaaact aaaagaatct ttcgagtata taataataga ttgtccagca ggtattgagc
    23521 aaggctttaa aaatgcagta gctggggcag atagagctat tgtagttact aatccagaaa
    23581 tatcagcagt aagagatgct gatagaataa taggtctatt agaagcaaat gaaataaaag
    23641 agataagatt agttataaat agaattagaa atgatatggt taagcgtgga gacatgatgg
    23701 ataaacaaga tataatagaa atattagcaa tagatttgtt aggtcttgtt cctgatgatg
    23761 aaagcataat tatatcaaca aataaaggag agccagctat acttgattct aagtcacttg
    23821 ctggtcaagc atacaaaaat atcgcaaaaa ggatactaaa tgaagaagtt cctctacttg
    23881 atcttgaagt tgaagatgga ttctttggta gacttaaaaa aatgtttagc atggctaagt
    23941 agcaaaggag gatacatctg tgttagattt atttagagtt ttttctaacg aggctaaaac
    24001 tagtaaatct gttgctaaag agaggttaaa gctagtttta gttcatgata gagtagattg
    24061 ttcaccacaa cttttagaga tgataaaaac agacattttg aaagtaatag ctaattatgc
    24121 agaaatagaa gatgatggtc ttgaaataaa aatgtctaaa tgcagaggtg agcatgatga
    24181 caaacctgta tctgcattag ttgctaatat accactaaag aatataaaag atagatgtat
    24241 gtaattagtg cttaaaaggg ggatatcctt attggtatat gcccctttta tttaattata
    24301 tgtttaaaga tagaaagacg gtgattcttt gaaaaaaatc aatattaatt ataagtcgac
    24361 aattaagttg ataaaacagt tagactggaa acttatagtt acagtattag ctatctttat
    24421 ttttgggctt gttatactta gtagtgcaac acatgctaat tcaacaggtt catataatca
    24481 attgattaaa caaggattag cttttgtact tggtatagga atgattatag tcatattatt
    24541 ttttgactat aaccttctag gcaggtatta taaagcgctt tatataataa gtcttatttt
    24601 gttagccata gttttattac ctggaattgg gacagtaaaa ggtggagcta ggtcgtggat
    24661 taacttagga ccacttgact tgcagacatc tgaaatagtc aaattaacat ttgtactaag
    24721 ttatgcaaag atacttgaat ccaagaagga taaattaaat acgttaaagg aagttatgcc
    24781 tgttgtagtt tattctcttc catttatcgg attattgatt gcacaaccag acttgggaac
    24841 aggaattgta ttttgttgta tgatatttgc aatgctgttc acagcaggtc ttagctcaaa
    24901 gttgataaaa agaggaataa tcatactatt agtgtcaatg ccactgatgt atttaatgat
    24961 ggcagaccat caaaaggtaa gaatagaagc ttttctaaat ccagaagatg taacactaaa
    25021 aggaaactat caagttatgc aatctctgat tgcaattggt tctggtggtg taactggtaa
    25081 aggactttac aatggaagtc aaaatcaaga agatttttta cctgtacaag atagtgattt
    25141 tatatttgct gttgtaggtg aagaacttgg tgtaatagga atggcagtgt tgataatctt
    25201 atttatgata ttcctattaa ggcttttagc aattgcaaga gatgcaaaag atttttatgg
    25261 aaccctcata gtggttggtg ttatgggaat gtttggttac cagataatcc aaaatatagg
    25321 tatgacggtt gcgttaatac cagtaactgg agtaacatta ccttttgtaa gttatggagg
    25381 aagttcgtta ttgacatctt tagcaaactt aggattagta ctaaatgtgt gtatgagaag
    25441 aaagaaaata aatttctaaa tttaaataca tctaaacata aattgaggag attagatata
    25501 tgaatatagc attagtagca catgaccaaa tgaaaaacac tatggtcgga ttttgtatag
    25561 gttatgaatc aattttaaaa aagtatggac tatatgcaac tggaactaca ggaaaaagaa
    25621 taatggatga aactgaatta aatataaata gattagcatc tggtccttta ggtggagacc
    25681 aacaaatagg ttctttaata gtaacacaag aaatagattt agttattttt ttaagagacc
    25741 cattgacatc tcaggctcat gaaacagata tacaagcgtt aataagactt tgtgatgttt
    25801 atcatgttcc aatagctaca aatctagctt cagcagaaat atttataaaa gctcttgata
    25861 gaggagaact atcatggaga gaagttagaa aaagcaaaag tcaacgtatt taaaaaatat
    25921 taatattaca gtctaaagaa acagatatat aagtgtttaa tgccctagtc tttctgttgg
    25981 aataaaccta gtacctgtag aaatcttatg aaagatttaa atgatgaaga gctaaatttg
    26041 agattaaata attaggaaat agattataga tagagtagta gaatttaaac tattactcta
    26101 ttttaataat atgaaataga atcaagtcta ttattatata tatttaaact tataggtaat
    26161 actatttcaa ccaagtaaat attgttatga caacattaat aaaacaaaaa tttattttta
    26221 aaatttgata aatagctgta tattagtatt tatagattaa atattaatat acagctattt
    26281 attattttta agtctattgt agaatataaa ccttgcatta tagagtacta tgtattatta
    26341 tagtaaatat aagtactata cattatagag tagtaggtga aaaggcggtg gtacataatg
    26401 cagttaaata aagaagtgtt aaaaggttat atcgatatat taattgtttc tatattagaa
    26461 aagaaagatt gttatggata tgaaattgca aagcaagtaa gagaaagaag tgagttcgaa
    26521 ttaaaagaag gaacaatgta tcttgcactt aaaagaatgg aatctaaaaa tttaattaaa
    26581 tcttattata gcaatgaaca aagttcaggt ggaagaagaa aatattataa tttaactaat
    26641 gaaggtaaag attttctaga aataaagaaa caagaatgga gatttattaa aaaggttatg
    26701 aatcaatttt tgggagaggt ggattaattt atgaaactaa tagatgaata tttagataaa
    26761 ctatataaaa aatgtgacaa taaaagtact atagaattaa aacaggaaat gagatgccat
    26821 cttatagaat ctgcaaatga atttaaatta gaaggattag atgaagaaga agcatgtaaa
    26881 aaagcaattg aaagatttga tgatggtgat gaaatgcagt atgaattatg taatataata
    26941 aaagaactat ctttatcatt ggataggcat aaatcaattg taatggggtt taaaaaagta
    27001 ttaggttaca taagtataat tgcattttta atttctggat ttatgtggta ttacaataat
    27061 agtttacaac ataatatgta taatttagga aaagaacttg atggagaaat aaagcaattg
    27121 gcagaaagac atgatatgac aaagattggt gaatataagt tagaacttga aaaaatactt
    27181 gataaagata agtatagtaa agtcaaagct cttagactat atgtaataga tatgaaagat
    27241 ggaaacacta atttaagttc ttctggattg aatgctaata tggtatatga gagggaagca
    27301 gattataata acataagtaa ttttatacag catttaggat ataatggaaa agatttttta
    27361 gacaagaatg ggaatatagt aaatccagat attttcttag aatatttctt ttattttgaa
    27421 tcggagatgt taattccagt agcatttgca tttggattat tatgtataat agcgtatttt
    27481 atattaagat ttaaaatttc tttaataaaa aataataatt aaataattca ccaaataatc
    27541 ttaatttgta gagtaaataa gatgtttcaa aataaaattt gaatcatctt attttttatt
    27601 gtttgttata ctcttttctt ttatatttct ccatagtgga ttgtatctat acataatcac
    27661 atattttgat ataattatac tttccaaaaa atgtattgaa tatacaaatt ataatctgta
    27721 taatgttgtt aggggagaat gtatgaagtg tatacaattt taattttaaa tgtattattg
    27781 acaattctcc tcactctcgc cattaccaat aattggaaaa ttgacatacc tgttacagac
    27841 caacattttt gaatatatta attattatca ttttattgtg atgatatcaa aaatgcaggt
    27901 ttgtgagttg gattgtatac gaactatata ggaaatatag aacagatttt ccaagaaatc
    27961 taaatattct atgaccacgt gaattgctat gcatacatgt tgataatcca tattttaaat
    28021 ctaaaatgaa cttaattaat ggtagggaat tgaaagtaaa aatatattat aaaggagaat
    28081 gctaatatga aaaaaatcag gaaattagct actaaactat tgattgcaat gatggtttta
    28141 acctcatgta tgagtgtaac agtttatggt atgacagcta aagatgttac tgcaaagaca
    28201 ccgaaaagtt atgtagctgg aaccaacagt atatatggtc caaagttatc acaagctcaa
    28261 cttaattcgg tagcacaagc aacagcagac tttatgaaca agaaaattac aaagaacatg
    28321 accacagatg ctaagattag ggttgcttat aatcacatta aaaacaacac aacatatgtt
    28381 aactggaacg cagcagaggg agccaatact gcttatacat tggttactaa aaaaggtgct
    28441 tgttctggaa tgtgtaggag cttgaaagct ttatgtgatg ctatgggaat tgaatgttat
    28501 tatgtacata gtacaacaaa tgaccatcag tggaatctta ttcgttttga ggatggacgt
    28561 ttgtatcatg tagatattga tgccaacaaa tcagcaggag cagacatcat atacaagagt
    28621 cttactcacc cattaccatt tgacaagaaa gcatatccag ctgttggcga agtagtaaag
    28681 acacagaatc caacgccaac tgttaagaaa cctacgccag ctaagccttc tgagccagtt
    28741 ggacctggga atcacactat gatagcagat caacaagggg caatacttga tgaagtttat
    28801 ggaaataatc tacgtccaaa aattgaaatg tatgagggag ttccgtgtag tttaatcaac
    28861 tcatcaataa atacaattaa gtttacaagc aaaaattcaa atttagaccc atcaaagcat
    28921 acagttactg taatagggtt tatgaataca ccatatggta tggttgcatt agctccagat
    28981 ggtgtacctg atgagtatcc ttttgaatat gataagccat tttctttaga ttttactggt
    29041 aagaaccttt ctgcagcgga attatcaaaa aagtgtaaag aaaataatgt aattattaaa
    29101 gtggaaattg gtgaaacggc atctaattct ggaagaggga tggtagcata cgctagatat
    29161 gtgaattaat acaaatatac tagtgtacaa atagaacata ttataaataa tgcaccctcg
    29221 cacaaagggt gcatttatca ttatatcagt cattttagaa aataaaaaag gagttgaact
    29281 taaaattaga ttagaaaaat taattttgag acaactccat tttaatttat ttttaaatat
    29341 aaaatcattt attttaaata atattatgtg tgattaatgg ctatttaact aattttgcaa
    29401 attctgcacc aaatgccata cactctttta atgtctcttc actagggaag aattttttct
    29461 taagagattc tacaggcatt ttgaatgaca tagatttaag taaagtctca gccatagtta
    29521 taccttcacc actccaacca aatgaaccaa atactcctgc tatcttgcct tggttagcca
    29581 ttgggtctat aacagagaat aaatcccaca taggtttaac catagttttg tttatagttg
    29641 gagagcctag taatattact tttgacacta ctaatgtatc gtgcatttct gttaatgtca
    29701 tatcttctaa atcgtataat tcagcctttg caccttcttt atcaagtcct tcttttatct
    29761 ttttagccat ctctaaagta ttactataag ctgataagta gaatatagaa acttgatttt
    29821 gattagttgt attaacagct tctgtagacc actcaacata tcttttaaca gcagccattg
    29881 ggtctttagt aagcattgga ccatgagaag tcaatatagt atcaaattct atgtttagtc
    29941 ctaccacctt atctacagca cttaaaacat gtttagcaaa tggcttaact atacaatcat
    30001 agtagtgttt agctgatttt agataatctt cactattcac tacctctgca tcaacacttg
    30061 caaagtgaca accaaatgca tcacaagtta aaagagtctt gtcctcctca atgtaagtaa
    30121 acattgtatc aacccaatgt aagaatggag cagttataaa tctaagattt cttttaccaa
    30181 tatttaaaat ttcaccatct tttattacat gacagttaaa aggtctattt atttgaccat
    30241 ctaagtatag tttagcagct ttagtacaat aaacttctat attaggattt atatctaata
    30301 agtattttaa actacctgca tggtctggtt ctgtgtgatg aattacaaca tagtcaattt
    30361 tagctatatc agtaacctct gataaattac ttaaaaactc atctttaaaa tttgccttta
    30421 cagtgtcaaa taaaaccgtt ttttcgtcct tgattaaata agagttgtaa gttgtcccaa
    30481 actcagtttc cattattatg tcgaatactt ttaaaccttc atctactact ccagtaaagt
    30541 agatatcttt ttttacttca aaaactttac tcatttttaa ccctcctaaa atttatcatt
    30601 atataagtaa cataccccat tttgaggtga atgaaacaaa agatgtcatg ataagtcatg
    30661 taaaacaata gttt
//
LOCUS       DAEORZ010000035        30025 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEORZ010000035 DAEORZ010000000
VERSION     DAEORZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.035"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            33..371
                     /locus_tag="KRH39_001464"
     CDS             33..371
                     /locus_tag="KRH39_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBG8511380.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(498..1787)
                     /locus_tag="KRH39_001465"
     CDS             complement(498..1787)
                     /locus_tag="KRH39_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG8511381.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            1867..2571
                     /locus_tag="KRH39_001466"
     CDS             1867..2571
                     /locus_tag="KRH39_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBG8511382.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQRNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2663..3709
                     /gene="selD"
                     /locus_tag="KRH39_001467"
     CDS             2663..3709
                     /gene="selD"
                     /locus_tag="KRH39_001467"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2711..2713,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBG8511383.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3727..5139
                     /locus_tag="KRH39_001468"
     CDS             3727..5139
                     /locus_tag="KRH39_001468"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBG8511384.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVIAITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            5253..7136
                     /gene="selB"
                     /locus_tag="KRH39_001469"
     CDS             5253..7136
                     /gene="selB"
                     /locus_tag="KRH39_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBG8511385.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7241..9979
                     /locus_tag="KRH39_001470"
     CDS             7241..9979
                     /locus_tag="KRH39_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBG8511386.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(10098..11042)
                     /gene="manA"
                     /locus_tag="KRH39_001471"
     CDS             complement(10098..11042)
                     /gene="manA"
                     /locus_tag="KRH39_001471"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBG8511387.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            11215..12576
                     /locus_tag="KRH39_001472"
     CDS             11215..12576
                     /locus_tag="KRH39_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG8511388.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLNINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TLEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            12664..14553
                     /locus_tag="KRH39_001473"
     CDS             12664..14553
                     /locus_tag="KRH39_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8511389.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISTVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14568..15023
                     /locus_tag="KRH39_001474"
     CDS             14568..15023
                     /locus_tag="KRH39_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8511390.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            15082..15399
                     /locus_tag="KRH39_001475"
     CDS             15082..15399
                     /locus_tag="KRH39_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG8511391.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            15424..16686
                     /locus_tag="KRH39_001476"
     CDS             15424..16686
                     /locus_tag="KRH39_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG8511392.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16791..17855
                     /locus_tag="KRH39_001477"
     CDS             16791..17855
                     /locus_tag="KRH39_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG8511393.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARNIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            17909..19087
                     /locus_tag="KRH39_001478"
     CDS             17909..19087
                     /locus_tag="KRH39_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG8511394.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVVCNTLDVVNDRYYINFDKLEIQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIEKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDIFRNKLKQNSITSPNVF
                     GIIGLITAYNECREWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(19278..20336)
                     /locus_tag="KRH39_001479"
     CDS             complement(19278..20336)
                     /locus_tag="KRH39_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBG8511395.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            20403..20774
                     /locus_tag="KRH39_001480"
     CDS             20403..20774
                     /locus_tag="KRH39_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBG8511396.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            20811..21485
                     /locus_tag="KRH39_001481"
     CDS             20811..21485
                     /locus_tag="KRH39_001481"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBG8511397.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            21734..22573
                     /locus_tag="KRH39_001482"
     CDS             21734..22573
                     /locus_tag="KRH39_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG8511398.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            22817..22913
                     /locus_tag="KRH39_001483"
     tRNA            22817..22913
                     /locus_tag="KRH39_001483"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22851..22853,aa:Sec,seq:tca)
     gene            23064..23771
                     /locus_tag="KRH39_001484"
     CDS             23064..23771
                     /locus_tag="KRH39_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBG8511399.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(23913..24749)
                     /locus_tag="KRH39_001485"
     CDS             complement(23913..24749)
                     /locus_tag="KRH39_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8511400.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            24937..25842
                     /locus_tag="KRH39_001486"
     CDS             24937..25842
                     /locus_tag="KRH39_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBG8511401.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DEKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            25851..27035
                     /locus_tag="KRH39_001487"
     CDS             25851..27035
                     /locus_tag="KRH39_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8511402.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            27150..28451
                     /locus_tag="KRH39_001488"
     CDS             27150..28451
                     /locus_tag="KRH39_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG8511403.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIESPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            28405..29448
                     /gene="holA"
                     /locus_tag="KRH39_001489"
     CDS             28405..29448
                     /gene="holA"
                     /locus_tag="KRH39_001489"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBG8511404.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(29635..29901)
                     /gene="rpsT"
                     /locus_tag="KRH39_001490"
     CDS             complement(29635..29901)
                     /gene="rpsT"
                     /locus_tag="KRH39_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBG8511405.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT        11961 a         3108 c         5032 g         9924 t
ORIGIN      
        1 aaatcaatat caaataaatg ggaggaaatc taatgaactt attagtgatt ggtggtcatg
       61 aaagaatgga aaaagattat atggttatgg ccaagaaaaa aggttataag acaaaggtat
      121 atactaccat gtcatctaaa ttaaataatt ctataggaag accagatgca gttgtcatac
      181 ttacatcaac tgtttcacat aagatgtcta gaacggttga gtctcaagct aaaaaacaag
      241 atatattaat cgtaaggcat aaaaatagta gtaaggtagc atttaatgaa tgtctagata
      301 tggttgatga atgtctggga aattgttcaa aatgtaagct taaagacaaa caaaaacaaa
      361 aaaatatata ggtataaaaa atacataaac ataaaattaa aagtactgag ataatagtta
      421 taattattat ctcagtactt ttagatttat aaggataagt atagtataaa ttttttatta
      481 taatttccaa ctacttgtta ctttttcata ttttttttgc ttctagcttt ttttcttttt
      541 ttcttcttta tatttgaagc tattataaat aaaactaaaa gcactagaat acctgcaata
      601 atcttaacaa aagtcattaa aacattattt tcagtcaaaa atggaagact gctatcaaga
      661 ttattttttg caattaagtc tatactgttt tctagtttac cattattata tacatttaaa
      721 gtaccaacct tagctccttt ttttataggc aaatcaattt tatttaactc tgttttggta
      781 tcataattgc cttgagattc atctttttct aagaatatcc tataactatt ttttggttca
      841 taaatcaatt catgttgttt tgtaaacaat acttttttag tatctgtgta tttttcttta
      901 tcaacaatag tagcacactt aaagttatca aatccataat caatcagaat acgtgagtct
      961 aaatataaat cagcattagt tgaattaaat acagcaacga taactcttct gccatctttt
     1021 acagcactcg ataaaagaca tttaccagca gcatcagtat atccagtttt tataccatca
     1081 actatatcat attttatatt tataggttga cctttgtatg taatcttatc atgtgatgtc
     1141 aaaaacttat tagtgtttac aaagtatctc tcataaggat atgcttttgt agcttcaaat
     1201 ttaagtgact tagtttttac aatttgtcta aaagtatcat tattcatagc ttctctagct
     1261 atgagagcca tatcatgagc tgttgtgtag tgatttgggt ctggtaatcc atgaggatta
     1321 ttaaaatgag tattagtagc tccaattttt tttgcctcag agttcatgag gtcaataaat
     1381 ttttgtacat tgccaccacc aacatatcta gctaatacaa aagcagcatc attagcagaa
     1441 tgaaccaaaa gagcatctag taactgcttt acagtaaaag attccccatt ttttagatac
     1501 atactagagc catctatttg aggtaaatct ttaatttcta ttacttgatt taaatcttta
     1561 acctccttta gaactaaaca agctgtccat actttagttg ttgaagctgg ataaagctta
     1621 gaatttccat ccttattata tagtattttg ccagactcat agtccattaa aactgcatgt
     1681 ttagcaacta tagctggttc attatctgca aaagagatat tagagcttcc taaaaatgaa
     1741 gtaaatatca ataaacagat taataaaagt gataaatttc ttttcattgt gagcctccaa
     1801 attttaaatt catcttttag tatagcatat atttgtcaat aattaaaaga aaggagtgat
     1861 taatatatga aaaaaaaagt aaaatttatt tcgttttttg ttttatttat tttaataata
     1921 tcatcaataa tcgtttataa atcgcttatt ttcaaagatg atatttatgt tgtaagtaaa
     1981 aaaaatttac ctaatgaaaa aacacatgtt gtagagaatg aaattaaagg tgaagataat
     2041 aatcacaata aagtaactaa agacatagat aaaaattttc aaaataataa aataactata
     2101 tacataagtg gagcagtaaa tagacctgga attgtaacta tagagtcaga caagagactt
     2161 tatgatgctg tagaattact aggaggaacg acaaaagaag cagatttaaa tggagtcaat
     2221 ctatcagtaa gattagaaga tgaacaacat tacataatac ccaaaatagg tgaagcaaca
     2281 tctgttacaa gtaatgatga ttccaataaa ccaaatcaga ggaatgagtc aagaggtgag
     2341 cctaaaaata aatccaataa aaatatatct aacgaatcaa aagtaaatat aaatgtagcg
     2401 actatagaag agttggattc tctacctggg gttggagaag ctacagccaa taaaatttta
     2461 caacacagag aagaaaatgg acagtttagt tcaattgagg aaataaaaaa tgtaaatggt
     2521 attggagata aaaagtatga aaatataaaa gatttaattt gtgtagacta aaatataata
     2581 caaaacttac atatagatat gttattataa atatgtatgt atatgtgtgt acatgtggaa
     2641 atttagtaaa ggaggcactt ttatgaaaaa agaacaaaaa aaacttacag agatgacaac
     2701 agcaggtggt tgagccgcta aaatagggcc agaggttctg gcatcagttt tatctcaatt
     2761 gcctaaaaat gataatatag aaaatttatt agtaggacta gatacagcag atgatgctgc
     2821 agtttataaa ttaaatgatg atatggcact tatccaaact ttagacttct ttacaccaat
     2881 ggtagatgac ccatacgtat ttggtcaaat tgctgcatca aattcactat cagatgtata
     2941 tgctatggga ggcaaacctt tggttgctat gaatattgtt tgttttccat catgccatga
     3001 tatggatgta cttgcagaaa tattaaaagg tgggtttgat aaagtgaaag aaagtggagc
     3061 acttttggtt ggagggcata ctgtagatga caaagagcct aaatatggtc tttcagtttc
     3121 tgggattgta agtccaaata aagttttatc aaatgctact gcaaaacctg gtgataagtt
     3181 aattataaca aaaccaattg gagtgggtgt attaaataca gccatgaaag aaggtatggt
     3241 agaacaacat atagcagata aagtaataga aataatgata catttaaata aatatgcagc
     3301 aatgagtttt gagaaatttg atgtaaattc ggttacagat ataactggtt ttggtctact
     3361 tggtcatact ttagaaatgg ccaaagcttc tgaagtaagt attgaaattg aatcaaaaca
     3421 tgtaccaatt attgaaggtg caatagaaat ggcacaaatg ggaattatac ctgctggtat
     3481 gtataaaaat atgcattatg tatctaaaga tgtagaagtt gtaggaaata tagaagttgc
     3541 agttcaagat attttatacg acccacaaac atcaggaggg ttgttaattt cagtaaaaga
     3601 agaacttgca gaggaacttg taaaagatat gaaattaaat ggagcaatag aagctaaaat
     3661 tataggttct gtagttccaa aaggggaaaa atatattaca gttttataaa atattataga
     3721 gaaagagtga ttgttttgag caaaagagaa ttgtttgcaa tgttgccttc agttgatgaa
     3781 gtattaagtg ataatagaat tgtagaaata ataaatgaat atcctagaag tttagtttta
     3841 gaatctgtga gagaagtaat agatttaaaa agacaattta tattaagatt aaaagaagat
     3901 gcatcaaact ctgtaacaat agagtttggt gaaataatag aatctgcaat agagagagtt
     3961 aagttaaatt actcattatc tttaaaaaaa gttataaatg caactggtac agttattcat
     4021 acaaatttag ggaggtcttt acttagtgaa gatataaaag atgaattatg gtgtgctgct
     4081 tcaaggtatt caaacttaga atacgatttg gataatggag aaagaggttc aagatactct
     4141 catttaacta gtacaataaa aagacttact ggagcagaag atgtactagt tgtaaataat
     4201 aatgcagcgg cagtacttct tgtacttagc acaatggcta aaggaggaga agcaattgta
     4261 tctcgtggag aattagttga agtaggtggt tcatttagga ttccaagtat aatggctctt
     4321 agtggtgcag aattagttga agttggttca actaataaaa ctcatttgaa agattacaag
     4381 gaggccatta cagaagatac aaatgtattg atgaaggttc atacaagtaa ttatagaata
     4441 atgggattca cagagagtgt ttctatagaa gaattggtta atttaggtaa aaaatataaa
     4501 ctgcctgtaa tagaagattt aggaagtgga gtttttatag atttatcaaa atacggttta
     4561 tcttatgaac caactgtatt ggattctatt cgacaaggtg cagatgtagt aacttttagt
     4621 ggagataaga tgcttggagg tccacaagca ggtattatag ttgggaaaaa agagtatata
     4681 gaaaaaatga agaaaaatca acttactaga gcacttagag ttgataagct tacaatatgt
     4741 gctcttgaag caacattgag aatgtattta gatgagacaa aagctataga aaatatacct
     4801 actttaaaaa tgttgacata taaaattgaa gaattagaag taaaagcaaa taaactattt
     4861 gaaaaaataa ctgctttaaa cttaaatgca aacataaata tagaagatgg attttcacag
     4921 gtgggaggag ggtctatgcc tcttgaaact ataagtacaa aggtaatagc aataactcct
     4981 gaacatatga atgtttcatc tttagaaaaa aaactaagac ttagtgaagc acatataata
     5041 gccagagtgt atgataataa atatgtacta gatgtaagaa ctatatttga tgatgaattt
     5101 gatgtaatag ttgaggaatt gagaaaagca tttaattaaa taaatttgag ttaataaatg
     5161 ttaaatttag aagtataagt atgaagtttt aattaaaaaa tagctacaca cacttgtgac
     5221 tttagtcata gcaggtttag agggggcaaa aaatgaaaaa tgtagtaatt ggaacagcag
     5281 gtcatattga ccacggaaaa actactctta taaaagcatt gactggaaga gagactgata
     5341 cattagatga agaaaaaaag agaggtattt caataaactt aggatttact tattttgatt
     5401 taccaaacaa tagaagagct ggaattgttg atgtcccagg acacgaaaaa tttataaaaa
     5461 acatgttagc tggagcatca ggaattgata tagttttact tgttgtagca gcagatgaag
     5521 gagttatgcc acagacagtt gagcatttag atattctaag ctttttaaat atcaaaaatg
     5581 gaatagtggt tctaactaag tctgatactg tagatgatga atttagagag ctagtaaaag
     5641 aagatatttg tgaaaaaata aaaggaactt ttcttgaaga tgctgaaata attgaagttg
     5701 attctatatc taaaagaggt atagacactc ttattgaaaa aatagataaa atgagtgatg
     5761 atatagaaga taaaaatgaa aattctccag caagacttaa tatagacaga gtattttcaa
     5821 taaaagggtt tggtactgta ataacaggca ctttaataga aggaaagata agtatagaag
     5881 atgatttggt tatatatcca caggtattaa aaacaaaaat aagaagtata caagttcatg
     5941 gagaaaataa agatacagct tatgcaggtc aaagaacagc aatcaatata tcaaatgtaa
     6001 aagtggatga tataaaaaga ggagatgttt tagctgcacc aaactcattg gaggaatcaa
     6061 tgatgttaga tgtaaaatta tcattggtaa atcatttaga taaaggatta aagcactggg
     6121 atagattaag attgtatcat ggaactagag agatactttg tagagcagtt ccactagata
     6181 aagaattaat tgaaagtgga gaaagtggct atgttcagtt gagacttgaa gaaagcatag
     6241 tatctaagaa aggtgatacg tttgtagtga gacgttattc tccaatggaa actataggtg
     6301 gaggtgtaat aattgaccca tctcctaaaa aacataagaa atttgatgaa aaagttatag
     6361 aagcattaaa aataaaagaa aaaggcgaac taaaagatat aatagaagag tacttaaaaa
     6421 ggaatttaaa aaattatcca aatataaaag aaataatgag ctatagtggt gctcatgaag
     6481 aagatgtaaa aagagcgctt gaaacactta tatcagaaga taaagtattt attataggaa
     6541 atatgtatat gcatataaat caatacaata aattaaaaga aaatacaata aagcttttaa
     6601 gtgagtacca taagaagtat aggcttagaa aagggatact aaaagaagaa gtaaggtcta
     6661 agatagagag taattttaag actagggaga tggatatttt attagaaaaa ttgtctacag
     6721 aaaatgctat aaaaatagaa aataatatag tatctctatt agattttgag gttattttaa
     6781 atgataagca aaaagaaata gcaaaaaaaa tagaaaaaag attaaaatca tgtggagttt
     6841 cttctatact gacaatagat gaagtttctg aaggaaatca taactatgct gaagtattgg
     6901 agtcaatgat aggtaataaa gtagagaaat tagatgattt gtatataatg gacaaagata
     6961 tttatgaaaa tgcaaagaac attttgataa attatataaa agaaaataaa gaaataacat
     7021 taggagaata cagagatttg attgattcta gcagaaagaa ttgcatgatt atattggaaa
     7081 acttcgatag aaacaaaata actaaaagag tagaaaacaa aagaattttg ttttagcaga
     7141 aaatgtctgt ttttgcttta aggtataaag aaggaattat aatgtcgagt atcgtataat
     7201 taattagtat aaaatattaa ttaagaggaa gtgtaaagta ttgaagaata taatacctga
     7261 tgtaaaacca gatataaaag aaagagaaga catgagcctt ttatttataa taatgggctc
     7321 agtactttta ttatttatta taaaagatat agagaaatat gaaactttat tacatgccat
     7381 atatacgata tttttagtag ctacaggagt aattatattt aatactacaa aatttaaagt
     7441 agattcattt tctacatttt taggattaat ttttatagct actggagtat tggagtgtat
     7501 atatttattt aattccttgg ggattaaaac taagtctata atggagatta acatcacaat
     7561 atcagcaata acagatttat ttcctatact gggtgtatac ttaagtttta aatttgttaa
     7621 ggataacaaa caaatttatt caagtgtaat tttatttata gcaactgcta cattaacaat
     7681 aacatcttta ttcataatat caggaatttg tgactatctg ggaaatggta aatcacaata
     7741 tgctttagga gttataatat ctatattcat aataattatt tctatcatta gtggaattga
     7801 attaaattca agttcaaata aaggtaaatg ggaatatgat gaaaaaaaga tttttaacag
     7861 aataatagta ataataatct tatcaagagt tccaaatcta ttgcatatag ttattgataa
     7921 tagaaatgta gagaggatat tatcacaaat aataataaat gtagccttat attatttata
     7981 taattacata gtatctaaga atattaagaa aacagcactg gaattgcatg atacgaatga
     8041 agaattaaca aaaaaaacgg aaagtttaaa agaaaagaat aaaaaattaa tctatgagac
     8101 agaaaagata gaggaactta agggaatact tatgaaaaga gaatctaggc tacaatctac
     8161 attagatgta gcagtaaata gcatagtagt atttagcaaa gatggagaaa ttacatatgc
     8221 aaataagagc tttagaaata cttttggaga ctataaagaa caagataaat tgactgataa
     8281 aataaaaaac ttcaatgatt tgatagaaag cattcattat gtgtttgttc atgagaagaa
     8341 tgcagagaaa cttattagta caagcgataa taaagtttat caagctatat ttgcaccttt
     8401 aattattgct tcacaaacag aaggagtatt gtgtgtacta gaagataaga ctaagaaaaa
     8461 ggagtacgaa cgcaaactta ttgaagcaaa taagagaagt aaagattttt tagaaagtat
     8521 tggagatggc atagtagtgc ttgaaggtaa taacaaaata tatgttaata atgcatgtag
     8581 ggaaattttt aaaaataatt tagagagtat tgatttttca ttagtatgta aaagtgaaga
     8641 aagtatagaa aaaagatatg taatagatgg tgaagtaaag tatgtagaga tgagtttttc
     8701 tcagtatact aatgctggta ctaataaaac tattattgta atacgtgata caacctctcg
     8761 aaaaatatct caaataaaac ttgaggaaag tcaatcatca tattcaagat ttatagatat
     8821 attacctgat ggcatatgct tattaaaaga agatttaagt ataaactatg caaataagtc
     8881 gtttttagat atgctagctt ttacaaacat agatgatata aaagattcta atattaaact
     8941 attaatgaat gcgaatactg aagaaaaaat gaaatttacg gataatatgg ctaaagtttt
     9001 aaatgaaaat agaagtatac ttttactgga atatgaattg ataaatagtt atgatgatat
     9061 agtagaagta gaattaagtg cattaccttt tgctatatac aatactagat acataatgct
     9121 tattataaaa gatttagtac ataagaaata ttccgagcaa gcagaaaaag aactgcttga
     9181 aagatttaaa acagataaaa tcaaaacgga gttttttgca aatatgtctc atgaattaaa
     9241 aaccccacta aatgttatat ctagttcaaa tcaactggta gattctttct atagaaatga
     9301 aaagataaaa gattacaata acaatattaa atctcatgtg gatttagtta gacaaagttc
     9361 ttataggctt caaagattaa taaataacat tatagattta acaaagatgg agtctgggtt
     9421 ctatacacta aaacttgcga aatacaatat tgtatctgta atagaagact tatttatgaa
     9481 tatagaagag tatgcactta gaaaagacat aaaaattctt tttgatacag accttgaaga
     9541 aattaatgtt tatatagaca aggttgaaat tgagagaatt atgttgaatc tattgtcaaa
     9601 ctgtattaag tttactgata atggaggctg gatatatgta agtattcatt ataaaataga
     9661 taaagtaatc ataagtgtaa aagacactgg agttggaata ccacaagata aattagaatt
     9721 gatttttgaa gaattttcac aagttgataa gactttatcc agaaatactg aaggaagtgg
     9781 aatagggctt gctatagtta agaatctagt aagtcttcat ggtggagata tagaagtagt
     9841 aagtgaagtg aataaaggca cagagttctt aatttcactt ccaatgaagg gatttagtag
     9901 tgaacattac acagaagata agagaatata taatattcaa gaaaaaataa aaatagaatt
     9961 ttctgatata tactattaaa aatgattatg agcaaaaata aaataaaaat actatttgtt
    10021 taaatattta agttgtaaga aataggctat cataaggtaa attattatga tagcctattt
    10081 ttagctaggt tttatatcta tatgtgagaa catataatct ctaaattacc ttcaaaacta
    10141 aaatctccaa agtcgtttgg tagtataaaa tgcataccct tttttaaatt gtattctaca
    10201 gaattgtgta taagttttcc atttccatcc aacacactgt ataaagaaaa aggtgtattg
    10261 tgtgtaaaat tacattttcc aaaaacatct aatttataaa cactaaaaaa ttcgtttgac
    10321 acaaatgttg tgcatttaaa gtctgatgtt gatacgattt tatagtcagt atcgaaattt
    10381 atatgaggaa cattcgtaac atctattgat ttttgaacat gaagttcacg cttattacct
    10441 gaattgtcag ttctatcata atcatagact ctataagtag tatctgagtt ttgttgagtt
    10501 tctaaaatca atgttccttt gcatatagca tgtatagtac cacttggaac atagaaaaag
    10561 tctccctttt ttatattaac ctttctaagt agtttgtccc attcattatt atttaccata
    10621 tttacaaatt ctttatgaga ttttgcatta tgacctatta taatttctgc gtcatcagaa
    10681 caatctatta cataccaaca ctctgtctta cctaactctc cattttcatt ttttttagca
    10741 tattcatcat ttggatgaac ttgaactgat aaattatcat ttgcatccaa tatttttgta
    10801 agtaaaggaa acttatcacc aggtgtgttt ccaaataatt ctctattttt attccacaat
    10861 tcagatagtt ttttaccttt atattttcca ttttctatta agcaatctcc atttttatga
    10921 gagcttatag cccaacattc accagttgtt ggcgaatcta tttcataatt aaatttatct
    10981 ttaagtgcag taccacccca tattctatcc ataaaaatag gttttaaaaa taatggttcc
    11041 ataatcctgt tcctcctatg atattaaatt tatcctacaa ataattatat ataaatagat
    11101 tataaaatca atagtaaaaa agttttgtcc cttgaaatga cataaagtat aaggagttat
    11161 taaataagat aaactatgat atataatata aagttctaaa ttggagtgat aattatggag
    11221 ttgtttaagg cagaaagcaa aaaatataaa aaagaatttt taaaattatt ggagcaatgg
    11281 gttagtatac cttcatttta tgatagaaaa actgtatcta aagacatgcc ttttggaaaa
    11341 ggagtatatg atgcattaaa ctggtttgaa aatctaggta gagaaaacaa tttcaaagta
    11401 aaaaatatag ataatcatgc tgttcaaatt gaatatggaa atggtaaaga gtatgtagat
    11461 atatttggac attgtgatgt tgtaaatcca ggtgaaggat gggatagtga accatttaaa
    11521 ctaaatatta taggtgataa attagttgct agaggtgtat ctgataataa agggcctatg
    11581 atagtgaatt ttttagcact aaaaatgata aaggatttaa atattaattt aaaaagaaag
    11641 gttagattaa tagctggggg aaatgaagag agtggattta aatgtataaa gcattattat
    11701 agcaaagagc catatggtgt atgtggattt actccagatg ctaaatttcc tgtattaaat
    11761 ggagaaaaag gtggagcaat aataaagtta atttcaaata tagacgataa atctttatat
    11821 ataagtggtg gaatagagtt taatacaata ccagataaag tttatataaa gaatgtagaa
    11881 aaattgggta aagataatat atgttttgat attaacaata tatcgataaa ttatgataat
    11941 ggcaattata ttgtgtatgg aaaaggtggt cattcatcta aacccgaaaa atcaataaat
    12001 cctatattag ctacaattaa acttttgtca gaaaatattg atgaaaaatg gacaaaagat
    12061 ttatataagt tgataaatca agataatata aatggaaact tatttggttt aaatattgag
    12121 gggaaatgtg ggattttatc aatggtgcca acaataataa atatagtaga tggaaaatta
    12181 gaagtagttt taagtgttag atatccagaa atattaaccc ttgaagatat aataaaaaaa
    12241 tttaatttgt atatggagca aaataatatt aataaatttg agcttattgg ggaaaattta
    12301 aagcaagcaa attatataga tagaaattca aaacttgtaa gaagtttgca tgatatatat
    12361 ataaagtatt ctggagattt aaaaaatgat gttagagtaa ctagtgcagg aagttatgct
    12421 tctgaaatga ataattcagt aatatttggt tgtgaatttc ctgatggtag ctttggcaat
    12481 gtccatagtg cgaatgaatt tgctagctta gatagattta taactgcaat aggtatatat
    12541 gcagaggcaa taattacatt atgtaataaa atataaatac aaaatgtccc ttatatagac
    12601 atattatata tacaattaaa attttacttg atatataatt gaaatcaaag agaaggtgat
    12661 acaatgataa ctaagaaaca tgcaataata gtaaagagtt taagcaataa agatggatat
    12721 atgacatcaa acgaattagc tatcaaattg gatgtgtcta ctaaaactat taaaagatat
    12781 atagcagatt taaatagtgt tttaagcaaa tatgatttgg taatagcttc ttctagaggt
    12841 ataggataca agttaagcgg ttctaaaaac aatatagcca gagctgttaa agaagcaaat
    12901 aaatatattg atggtttttt agatgactct gaagagtcaa gaatgtcaaa tataatatgt
    12961 atgcttatta atagaaatta tatgagtata gaagcaatgg cagaagaatt aaatctaagt
    13021 atagccgcta taaataaatt gtctagtaag cttaaaagaa aacttgaaaa gtatgactta
    13081 gttataaaat ctaaacctta ctatggaagc tatatacatg gtgaagaaat taatataagg
    13141 caattaatta ctgactatgc aataaaatta gatgaaaaaa gtaaagtgaa ggtgttttta
    13201 gatgacatta gtgaaaacga tatacactgt atagaaagta ttttagaaaa gcacttaaga
    13261 gaaaaagata caataatttc agataaagat tttaatcttt tgctttcaaa aataatcgtt
    13321 tctgtattta ggagtaagag aggtcatagt aacaatataa acttaatgga tacaagttat
    13381 agatttcata actactactt tatagaaaat ctaatgaaag aaatatctga taaaattgga
    13441 tttaaactta ttgaagatga agtaatttat atttcaaatt attctggtgt aatagcctat
    13501 aaaggtacac aaggaagaaa aaacacaagt gaaattgagg aacgaataag tactgttata
    13561 agtagtgcac ttcaagatat attccttatt tcaggtagtg attatacaaa agatgatgag
    13621 tttatggttg caatctctga ccatataaag agatttttaa atagagctag agctaatgta
    13681 aaatcaaata atcctttact tcatcaaata aaagagaagt ttcctatagc ttttaattta
    13741 gccgtgtttt tatcaaataa actagaaact gagtttaatt taaaacttga tgaagatgag
    13801 cttggatata tagctatcca ctttgctgct tcaaatgaaa gaatgaaaaa aaatactagt
    13861 aagaaaattt gtatagtttg tcattatggg ataggaacag gacaacttat ttctgaaaag
    13921 ctaaaacaga atataagtga tttaagtgtt gtgggtgttt atccagttag atatctcgat
    13981 atggctataa gtcaagatgt tgatttgata gtatctaccg ttgaattaaa aggatatgaa
    14041 aaacctgtct tatatataga aaatatattt gatgatagct taattgaaaa cgtaaacaaa
    14101 gccttttatg aaaaggaaga aagaagaaag ataataagca acatgtttga tgaaaaagct
    14161 tttttcagta taaaggctaa tactaaagaa gaggtcataa tttcactttc aaataaacta
    14221 aaagaaagag attttataga ggaaagttct ataaaaagta taatagatag agaaaatatt
    14281 tcatcaactg aaataggaaa tctagtagca ataccccaca caatagtgaa gggagataaa
    14341 aaatctataa taggagttgg aatacttgaa aatccaataa tttgggataa gcaagaggtt
    14401 cagctagtat ttatggtatt ttttaatact aaagaaaaac ataatttctc gatatttaaa
    14461 tacctttata attttataaa agatgaaggc ggagtaagag gaattattaa aatatgtgac
    14521 tttaataaat taatggcact gataggtaat taaggaggaa attaataatg ggaacaaaca
    14581 tatttaacaa agaatatgta tttttaaatg ttgatgctaa gtcaaaggtt gaagttttaa
    14641 aatttatatc aaaaaaagct aaagacttaa acttggcaga agatgagagc ttagtatatg
    14701 aaggtttaat ggctagagaa gaacaattta ctacaaattt aggagaatct atagcaatac
    14761 ctcacactaa aaatgatgca attgaaaatc cagcagtagt agtattgaag tttaatgaag
    14821 atgttgtttg gaatgaaggt gaggataagg ttaaattagc aataagctta cttatgccag
    14881 gaaaaagtaa agagaatatt catcttaaat tattatcatc tctttcaaga aaattaataa
    14941 ataaagaatt taaagattct cttttaaaaa gtgataatgt tgaagaaata tctaacttaa
    15001 taaatgaagc tttaggttta taattaaaca ctaaggttat aaaataaaaa aataattaac
    15061 taaaaatagg aggaatttga aatgaataaa aaattagtag cattatgtgc ttgtccaatg
    15121 ggactagctc acactttcat ggcagcagaa gcaatagagc aagcagcaaa ggcattaggt
    15181 tatgaagcaa aagttgaaac tcaaggtgct gatggtgtac aaaatgagtt aacaagagat
    15241 gatatattag gagcaactat gataatacat gcagtagcaa taacaccaga aggtatggaa
    15301 agatttgatg gttgtgaagt ttatgaagtt gaattacaag aagctataaa aaatgcagaa
    15361 ggtgttataa aagaaataga agaagattta ggaatataat aaatttattg tggaggaaat
    15421 agaatggcaa ttaagaaaaa agttataaac agttcaggtg cttctacaac aggaggaaat
    15481 aatgtaggaa aatctactcc atctagtcca actcaatcaa agggaaatgg aaaatggaaa
    15541 gaaataagta aacatataat gacaggtata tcatatatga taccagtttt agtaatgggt
    15601 ggacttatag gggccttatc tcaattaata ccatatgcta tattaggact tgacccatct
    15661 gtaggtatag ttgatgctat gaactcagga gaatttacag ggttcaagct tagtctttta
    15721 aatatagcac aattaatgtc aaatttcgga tttactttgt ttggatttgc gataccactg
    15781 tttgcagcat tttgtgcaaa ctctataggt ggaaaaacag ctttaattgc aggatttata
    15841 ggtggatata tagctaataa acctgtaggt gtaccacaat tcgttgatgg acaatggact
    15901 gaagttgttc cagttgcatc aggattttta ggtgcaatac taatagcatt tataatagga
    15961 tattttgtaa aatggttaaa taaatctata aaagtttctc ataactggtt agcttttaaa
    16021 acaacatttt taataccatt aatagcgtca ttagcttgta tggtattgat gatatttata
    16081 ataactccat ttggtggatt aattaatgaa agtatgaaaa acttcttaac agcagcagga
    16141 gcagctggag aatatgtata tgcaacagct ttggcagcag ctacagcatt cgacttagga
    16201 ggaccaataa ataaagcagc aggattcgtt gcacttggtt taactacaga gaatgtactt
    16261 ccaataacag caagaacaat agctatagtt gttcctccaa ttggattagg attaacaaca
    16321 ttattagata aaagattagt aggaagaaga gtatatgata gacaattcta tcaagcagga
    16381 aaaacttcta tattcttatc atttatgggt atatctgaag gagcaatacc atttgcactt
    16441 gagagaccag gttttgttat acctttaaac atagttggaa gtgtaatagg agctattaca
    16501 ggtataattc taggagcaat tcaatggttc ccagagtcag ctatatgggc atggcctcta
    16561 gtagataact tatttggata tataataggt atagcagttg gtgctatatt catagcagta
    16621 ggaaatatat tctatagaaa taaattaata aaagatggta aacttgttgt agattatatc
    16681 gactagtatt taatttttac tatctataaa taactttggc tggtaatatg gttttaatca
    16741 atattaccag ccttacctat atgattaatt aaaggcggtg gagataagat atggataaga
    16801 tagaaaaagt aagagagtat tttagagaat atgatataga tggatttctt ataaatagtc
    16861 ctacaaacaa gttttatgtt ggaaatttat ttagctcttc aggttatgtt tttataacga
    16921 aagaatcaca atatataata gtagatttta gatattttga agaaattaaa agaaaatcta
    16981 gtttgtttaa tgtagtatta atggacaaaa ctagaactca ttttgatata attaatgata
    17041 tttgtagaga gcagaatata aaggaaattg gatttgaggg aaatgaagta tcttttgatt
    17101 tatatagaag tatgagtaat aagttaagtg caaccttaaa atcagttgat ttatctacat
    17161 taagggaaac aaaaaatgaa gacgaaataa aatatataaa aaaagcttgt gaaatagttg
    17221 atgctacatt ttatcatata gtagacttta taaaggttgg tatgactgaa aagcaagttg
    17281 aaaatgaaat tgttagaatt ataaaagatt taggtggtca aaaagaatct tttgatacaa
    17341 tagttgcatc tgggcttaga ggagcattac ctcatggaaa agcaagtgaa aaagttatag
    17401 agtatggaga ttttgttact tttgactttg gtgctaagta taataattat tgttcagata
    17461 ttaccagaac catttgtatg ggtactataa ataaagagtt agaagaaata tataatatag
    17521 taagaaaagc taatgaagag tgtataaggg ttttaaggcc gggaatgact actggagaga
    17581 tagataaagt tgcaagaaac ataattggtt catatggata tgccaataat tttggtcata
    17641 atctgggtca tggtgtagga attatggttc atgaatatcc tgcattagct cctgaatcaa
    17701 atgaagtatt aaaagaagga atgattgtaa ctatagagcc tggtatatat gttccaagtc
    17761 ttggaggcgt aagaatagaa gatgatgtac ttataacaca agatggatgt atgagattga
    17821 ctacatctac taaggattta atagttgtaa gctaaaaata aattagataa atattttata
    17881 acaataagaa aagaagaggt aaatttaaat gtataatttt gatgataaac cagatagagt
    17941 aagtgaaaag tgtagaaaat gggatttaaa tattataaga gataaatttg gagatattag
    18001 agaagatttt ataccaatgt ggattgctga tatggatttt aaaataccaa cagaaataga
    18061 aaacaaattt attgaagctg taagaagagg tgtatttgga tatacatact gttatgatga
    18121 attttatgat tcagttattg gttggcaaaa aaatatgcat gaggtaaatg tagaaaaaga
    18181 atggattaca ttaacttatg gaactgtatc aacacttcat tatgtagtac aagctttttg
    18241 taagaaaggt gatagtataa tattaaatac accagtttat gacccttttg aatcatcagc
    18301 aaaaaaacaa ggtgtaaatg tagtttgtaa tacactagat gttgtaaatg atagatatta
    18361 tataaatttt gataaattgg aaattcaaat taaggaaaat aaacctaaat taatgatgtt
    18421 ttgtacacca cataatcctt ctggaaggat atggacaatt gaggaaatga caagagtagc
    18481 aactatatgt aaagaaaata atgttatttt ggttgcagat gaagtacatg cagagcatat
    18541 tcattatggt aaatttaatt caatacttaa gattgaaaaa gagcttttag aaaatgttat
    18601 tctattgaca tcaccaaata aaggatttaa tcttggtgga ttaaagactt cttattcaat
    18661 agttataaat aaggatataa gagatatatt tagaaataaa ctaaagcaaa attctataac
    18721 ttctccaaat gtgtttggaa taataggatt gataactgct tataatgaat gtcgtgagtg
    18781 gttaagtggt gtaaatgaat atataaaatc aaattatgaa cttttagaaa cttgggtaaa
    18841 taaatataat aagataaaat tgatgaagat ggaatcatct tacttagctt ggatggatat
    18901 aagtggatta ggtattagtg caagtgagtt tacagataaa ttggctatag atacaggagt
    18961 attattagaa gatgggagtc attttgtaaa agatggtgag aagtatgtta gaataaattt
    19021 aggaactcaa aaggaaaatg taattgaagc tttaaataga atggatttat ttttaaagtc
    19081 tttataaaaa tttggttgaa ttagtatttt atataaattt aaactattaa tatggtatat
    19141 acataagtag aatttgtttt agattactta agtatatacc atattttata taaaaattaa
    19201 atttgcatta gtcatgtgat aactatttaa attcatatgt attttgtatg tataatagat
    19261 ataattatta tttaaactta ttgtttattt aatttttcaa tagaaaaaga tttatctgta
    19321 agtttaaaat tatcttttat acccttagtt aagtatactt tcttatcctt agttatgaat
    19381 atagcatcta cattatcaat tttattaatt aaattcatac ctttttcaag accaagtcca
    19441 aatgtagaag tggataaggc atcacaatta atagatttat cagagattat tgtaacacta
    19501 cttaaattgt tttcaaaagg ataccctgta aatggattta acatatgatg atatattttt
    19561 ccgtcttttt cgataaatct ttcataaatt ccagaagtaa caacagattt atttgtagtt
    19621 tctatgtttc ctatggattc accattttta ctagtggggt cttgaatacc tattttaaaa
    19681 ggtttatcat ttttaccatt ttctaaagta aatatatttc ctcctaagtt tactaaacct
    19741 ttttttacgt cctctgattt taaataggct acaattttat cagcagcata tcctttagct
    19801 atggcaccta aatcaatttt catattttct ttggttaatt ttatagattt atttttttca
    19861 tccaatatga tgttcttata atcaataaga ggcagaagtt tttgtatctc agaatcactt
    19921 ggaactttag ctttatctgt tccaatagcc cataaatttg ataatggacc aattgttatg
    19981 tcaaaatatc catctgataa tttggaatat tgtatagctt cttttacgac gaaaaatgta
    20041 tcatcagaaa ctctgacaaa ttttttacca gcattatcat ttattttaga aacatcactt
    20101 cctggtatat gagtgctcat tttgttgtca atatatctta aaattgaatc acattcattt
    20161 aagattttat cacttttgga ttcttttaca ttaaatacag taacttcgtt tactgtacca
    20221 aggtagtagc tagttttact atatttagta ttctggcttg aactcctaaa aaatgaaaat
    20281 aaaattaatg ctaaaatcaa tatcacaaaa ataaatgtga tttttttctt attcatggta
    20341 tattcctcct atgaaattgt gttattattt tattataaca taatttcata ggaggacaaa
    20401 aaatgaagaa aaaagacatt cttcttattt taggcatttt ggttgttatt acagcatgtt
    20461 atgggataat aaatgttata aattctaaaa atgctggaaa tattgagatt tatgtagata
    20521 ataaattgta taaaactgtt tcaataaatg ccaaagaaga atttaaaata gagaatagag
    20581 gggggtataa catagtaaag attcatgata aaggggttga gatagtagat gcttcatgcc
    20641 ctgataaggt ttgcgtacat actggattta taaacaagcc aagtcaaagc atagtttgta
    20701 tacctaatag agtgagcata aaaataaata caaatgaaaa gaatgataat caagaagata
    20761 taatttctaa ataaaataat aaaaataatt gaaaggaatt ggtgataatt atggttaact
    20821 taggaaacga ttgggatgaa ttattgaaag aagaatttga aaaagattac tacttaaact
    20881 taagaaaatt tcttatagaa gaatataaaa cacgccaaat tttcccaaat atgcacaata
    20941 tatatgaagc tttaaaacac acttcataca aagacacaaa agtcttaata ttaggacaag
    21001 acccttatca tggagataat caagctcatg gtttagcttt ttcagttcaa ccacaagtga
    21061 aaactccacc atctttatta aatatgtata aagaattaaa agatgattta ggttgtttta
    21121 tacctaataa tggatattta atgccttggg cagaccaagg agtgttactt ttaaatactg
    21181 ctcttactgt tagagcgcat gaagctaatt ctcataagaa caaaggatgg gaaattttca
    21241 ctgatagagt aatatctatt ttaagtgagc gtgaagaccc agttattttt gtactatggg
    21301 gaagtaatgc acgtaaaaaa gtagagttga ttgatacaag taaacattat attttggaag
    21361 cacctcatcc aagcccattg tcagcaagta aaggcttttt tggatgtaaa cacttctcta
    21421 agataaatga aattttgaaa aagctaggaa aagaacctat taattggcag atagaaaata
    21481 tataaaagta aaaggtaaca aaatgataac aaaacaacaa taaacaatga cattgttgta
    21541 actaatagaa atatgcttaa atagttgaaa atactatgta tgcatatttc tatttttttg
    21601 tcaagttgac aactcttgaa aaaatagtta caatttagtt acacaaaaaa cacaaataaa
    21661 aaaagaaact tacaaagcag aattttatga agcaaaatat atagtgtaaa aaatcttagg
    21721 gaggaaattt tatatgaatt ttaaaaaatt gagtaatgct aaagtctcgg taatggcagt
    21781 tgttttatca gttggaatat tatcgggata ctctgcgctt aataaacaag taacattagt
    21841 agtaagggga gaagaaagaa ctatatcaac atttagttct aatgtaggag accttttaga
    21901 atctcaaaac attagctatg atgcaaatga tatagtaact ttagatgaaa atacaaagtt
    21961 atctaatgga gataaaatag aagtaattga tgtaaaggaa aaaacagttg tagaaactaa
    22021 agacattcca tttgaagtta cagttgttga agatggctct ttattaaagg gtgattctaa
    22081 ggttaaagaa gaaggaaagt ctggtaaaag tgaattaata tataaagaaa cttatcacaa
    22141 tggaaagaaa gtagaaaagg aatttgtgaa aaaggttgtt aaagctgagc cagtaaataa
    22201 ggttgtaagc aaagggacaa aggtagaaat gcaagtagcc tcatcaaggg gagaaagttc
    22261 aagaagaatg gcttcatcat catctagtag cagtagtagt aacaatggta aaaatatgag
    22321 agtggtagca actgcatata ctggtcacag tataactgca acaggaacaa aacctaagtg
    22381 gggaacaata gctgtagacc ctaaggttat accatatgga actaaagttt atatacctca
    22441 atttgacatg gtgtttactg cagaagactg tggtggagca ataaaaggaa ataaaattga
    22501 tatctacatg aatgattcaa aaacagttta taactgggga agaaaaacta tagatatacg
    22561 tatattaaaa tagttaaata atttatattt ttactggaaa ttatattttg attatataga
    22621 gaagaaactc tttatagatt ctaaaaatat aatttccagt tttttatttt ggatatgtaa
    22681 taaaacaggt aaattttaac catgtataga taaaagctat ttaaattata atttactatc
    22741 atataaatct ataaaaattg ttgcaaagag tcaaaatata tgatattata gatttcgttg
    22801 agaaggattt cattatggga gtggatgggt gccggtgtgc cctctagtct tcaaaactag
    22861 tatgaggggt taagagcctc ttaggtgggt tcgattccca cgcattcccg ccaatatgta
    22921 aaaggtatct tttaattaaa agataccttt ttaaatttat tttaagataa tttattttta
    22981 ataatatata atgttttgta ttatataatg taaattttct aatactatta tatatttaga
    23041 taaaaatcta ggagtgtgga aagatggaaa ataattttac aacgagaact agttttctag
    23101 ttggagatga tgggataaaa aaacttaata attcaaatat aatagtattt ggagttggag
    23161 gagttggtag ttttactgtt gaagctttag caagagctgg agtaggcaat attactattg
    23221 tagattttga tgatgtagat ataaccaata taaatagaca aataccagcc ctacattcaa
    23281 ctgtaggaag atacaaagtt gatgtcatgg aagaaagaat attagatata aatcctaata
    23341 tcaatataaa aaaaataaga agtctttata ataaagatac tagtgacgaa atactaactg
    23401 aaagatatga ttatgttgtg gatgcgatag acatggtaag ttccaaaata catctaattg
    23461 aaacttgtga aaaaaaagga ttgaaaataa taagttctat gggtatggga aataaattag
    23521 acccaactaa aatagtcgtt acagatatac acaaaacaag tacttgtcct ctagctaaag
    23581 ttatgaggaa agaacttagg gatagaggta tcaaaaagtt aaaagtagta tattctactg
    23641 aacaaccaat agaacttaag aagaaagtaa tgaatggtag aaaagtaaca cctggtagtg
    23701 tatcatttgt cccatctgta ggaggtttaa taatcgcatc tgtaatagta aatgaactat
    23761 taggacaata agttttaaat attttaattt ataaatttta tatacaaatt tgaaggctag
    23821 aagcatgaat acaaagcaat tttatattca tacttttagc ctagttattt tatattacct
    23881 tattttatgt agttgtagtt ttatttttta gattatgtat ctatttcata attatatttt
    23941 gtaaatataa tttctccatc actataaaaa acaatatctc ctattctgtc tgttctataa
    24001 attgatacat ggttttgttt tagattatta ataacctccc tatgtggatg accatattga
    24061 tttttatatc cacatgatat tgcagctata tctggactaa ttttttttat aaattctaaa
    24121 gatgaagaag aattacttcc atgatgacct atttttataa aattaatatt atttaattta
    24181 aaagaatgta aaatttcttt ttcattttct tcttcagcat cacccataaa taaaaaagaa
    24241 ttatcattaa aagttaattt gaaaactata gaatttaaat tagattcttc ttgtatatat
    24301 gatggactta atacatatat atttatattg ttatctatgt ttaaaacatc atttttatag
    24361 agatgctgaa tagataagtt tttatccgta caagaattta ttagattttg ataagcctca
    24421 ctatcagttg attgttcagg catatatatg gagtttacat taaatttttt tataatatta
    24481 tcaagacttc ctatgtggtc tgaatctgga tgagttgcta ttataatatc aatagttttt
    24541 attctttttt gtctaagata actaataatt atattttcgc tatcttcatc acctccatca
    24601 attaatatat tcttatttgt gggtgtttga actaaaatac tatcaccttg tccaacatca
    24661 atcatatgta tagatagtag agattttttg tcacagccga ttaaaagtga tattataata
    24721 agtattgata aaaaaatttt atttctcaat aaaaaatcac tccattcatt acatgtttat
    24781 ataaatttat gatgggtaat ataagatata tatgacttat tatattttat attttaaaca
    24841 atataaatga aattaatacc ataataaaat tggagttttt tatatataca aaataaaatt
    24901 tttaaaatta agcaataaaa aaggtaggag gattttatgt ttttcaaaga tattgaacta
    24961 aattcaaaaa aagagttaga cccatatttt gatttagtag attatgaagc ttgtgagtac
    25021 tgttttagta ctttatatat gtggcaacat gtttataaga caggatatta tataggtgaa
    25081 gattttgccg ttttagttgg ggaatacgaa ggagatagct tctcaatatt accacttgct
    25141 aaaaaagata aacttccaga agttgtagat tttgtccttg aatatttttc taaaaataat
    25201 aaaaaaatat acttaagagg tattactact gaagtagtag agtttttaaa agaaaaatat
    25261 ccaggtagat ttgagtatat agaagaaaga gatttatttg actatattta tgatgcagaa
    25321 agtttaagaa ctctagctgg taaaaagaat caaaagaaga gaaaccatat aaactatttc
    25381 ttaaaagagt atgcaggtag atatgaagct aaattattag ataaagaaaa ctttgatgag
    25441 tgtttagtac taatgaagga atgggaaagt aataaggaag aaaataatga atttgatgaa
    25501 agtatggatg atgaattaat aggtatcaaa aagatattta accattatga catattaaaa
    25561 gataaggtaa aagtgtttgg tgtttatgta gatgaaaaat tagaagcttt tagtatagga
    25621 gagcttttaa atccaaatat ggcattgatt catatagaaa aagctaatcc cgatataaga
    25681 ggtctgtatc catttataaa tcaacaattt ttggtaagtg aatttaagga cgttgagttt
    25741 gtaaatagag aagaagattt aggtattgaa ggattaagaa aagctaaact ttcttatcat
    25801 ccttgcagat ttgttgaaaa gtacagtgtt agggaggctt aatagactat atggagatta
    25861 ggtatgctaa agaagaagag atagaaagta taaaagagat ttggagttat tgttttaacg
    25921 atacggagag ttttatgaag tactatttta atgataaata taaatctgaa aatactgtag
    25981 tagctttaga tgaaggaaaa attatttctt ctcttcagct aaatcagtat aagttattac
    26041 taaatagcaa ggtatataat acatcatatg tagtaggagt ttctacactt ccagagggaa
    26101 gaggagctgg atatatgaac aaagtaatga aatttacttt aaacgaattg tataaaaaag
    26161 ggcaattagt ttctatattg atgcctattg actatagact ttatagaaga tttggatatg
    26221 agcattgtta cgaccagatt gaatatacta ttaacacaga tgatttaaaa aattttaagt
    26281 caagtggaaa aatgataaag tctaatttat cacaaattga tgatttgata caaatagata
    26341 gaactttttt aaatgaggtt aatggtaatg tgttaaaaga tgaacattat tatgaaaact
    26401 tatttaaaga gattcaaagt gaagatggat ttttatacat tcatgaagga aatgaaaaag
    26461 atggatatat agtttatttt cttcaggaag ataaaatgtt tgtcagagag ttattttata
    26521 aaaatataga tgcccttaaa tcaatgctta aatttatata taatcacaat actcaatgta
    26581 agatagttac tatatctact cctacaatag ataagataag atttatatta gacaacccta
    26641 aagatagtga tataaaaata aaacctttta tgatgggaag agttataaat gtaaagaagt
    26701 ttattgaaga tatagatatt gagaatgata ttaatagctc atttaattta ttaatagaag
    26761 acaagtttat agatgaaaat aatggtctat ttaaaatttc tatacagaat aagaaggtat
    26821 ctgtagagca gttagataaa aaaggtgcag aaaagcctca agaagatttt gatattaaat
    26881 tagatataaa taccttaaca cagttatcat tttcttatat agatgtaaat gaagctattt
    26941 tcttaaatga tataaaagat gtttcagaag aaacactaga aacacttaat tgtatttttt
    27001 ctaaaaaaaa taactatata aatgaatata tataggtaga gtacgttaaa agattttaaa
    27061 aatcttttga cgtatttttt tgtttagata acaatttatc ttgtttaaaa attttctatt
    27121 tgaaaataat ctaaataata tacttttata tgggagtgat tataattcaa ataagaagac
    27181 cattattaat tatattaatg tttgtggtag ctatatcatt tatcattaca aataacacaa
    27241 aaaacgacga agaacttaat gataagataa taactataca aggagttgtc aaaggaaaaa
    27301 ttgaaaagaa aagatacaat cagtacaagg taggggtttt tctaataaat gattatacaa
    27361 aaaataaaaa tttgaagatt ggtcagaaag ttaatattac aggtaaattc aaaagtttag
    27421 ataaaatgaa gtatgatgat tttgattatg gaaggtatat aaaaagtact ggatataaag
    27481 gtatagtgta tataaataac tataaaatta ttgataaaaa taaactttac tctttaatag
    27541 gggaaataaa gttttatatt agtaaaactt ataggtactt atataagaaa aactcagatt
    27601 ttataaattc tatactatta gcagaagttg aaaatttgac tgaggaacaa aaagaaattt
    27661 ttactagaac tggaactagt catgtaatat ctatatcagg tcttcataca ggaatattat
    27721 gtgtaattat atcctttcta ttaagaggta taaataaatt gtataaatta ttaatactgt
    27781 cgatttttat aactttatat tgtataatgg taggtgcgtc tccatccata attaggtcta
    27841 tagcatttgt tatgattttt tatttatcaa tatttattga tagaaagaaa gatggaatat
    27901 cggcattgtc tctaattggg ataattttaa ttatgaataa cccctatgtt atatataatg
    27961 taagttttca attgtctttt ttagctacac tttctatatt aatattctat aataaaataa
    28021 atagtattat aaaattaagt atggtatcat tgacaatatc atcaaatata ttaactttac
    28081 ctataattta ttatacattt aaaggtatac cattgctttc aattatagga aatttaatta
    28141 tagtaccatt tgtgggagta ataatgtatt taagtattgc aagtttaatc gtatttaaag
    28201 taagtgtagt aatagctaaa attatatctt tttttaatag tacattgata gaaagcatat
    28261 tttttttact agaaaaaatc agcaatttaa gttttgcata cattaatata gaaagtccta
    28321 aattttatat tgttgttatt tattacatag gagtgttttt ttacatattt tacatagaag
    28381 gaaaagagat aaaggagcaa gagaatgaat tacaaggata ttataaggaa tgtaaaagag
    28441 aaaaactttg aaaaaatgta tcttttttat ggaagagagt attatttaat tgagaatgca
    28501 ataaaagcat ttaaagatag tctaaacgag ggaatgcttg attttaattt agatataata
    28561 gatgggaagg aaatagtctt aaatcagctt ataagctcta tagaaacact tccatttatg
    28621 gatgatagga agatagtaat aataaaagac tttgaactat taaaaggaaa gaaaaagaat
    28681 tttacagata gtgatgagaa atacttaata gagcatttag acaatattcc agacaccaca
    28741 actatagttt ttgttgtata tggagatgta gacaaaagaa aatcattagt taagaaaata
    28801 ggaaacaatg gaattgtttt tgattgtgat aagctttctg atatggattt gtttaaatgg
    28861 ataaaaaaat cttttgcttt aaatgatgtt ataatagata attctcaaat catgtatttt
    28921 attgaacaag aaggatacag agataagagt agtgaaaaga cattatctga tttagaaaat
    28981 gaaataaata aaataagttc atttgttggt aaagggaata atgtaaccaa tgatgtaatt
    29041 gataaattat ctcaaaaaaa agttgagaat gatatattca aattaatcga ttatattgga
    29101 gaacaaaatg cttctaatgc aatgaaaata ttaaatgata tgatacaaga aggcgaatct
    29161 gtattaggta tattttctat gatagcaaga caatttaaaa taattatgca agtaagacag
    29221 ttacaacttg atggatattc aacaaaactt atagctgata aattaaaaat gcatcaattt
    29281 gttgtaggaa aagcattaaa gcaaactaaa aatttttctg atgatataat tgtggaaata
    29341 ttaaactata tattagaaag tgattataaa ataaagacag gtcttataag agatacttta
    29401 gcagttgaaa tgttggttag tagatactgt aaaagagaag caatttaaaa aatctatata
    29461 ttaataaaaa ataaatagca agatatgtta tagttcagag tagatgtaag ctaaattaag
    29521 tttgatatac aatcaactat aatatgtcta actatttatt ttttattgta taaatttaca
    29581 aaataaaaaa cccctacata ggggttatta tatataagaa atataaactg gtaattaagc
    29641 gttcataccg tttaattttt gagctaattt agctactttt ctagctgcag cattcttatg
    29701 tatagttcct ttagaagcta cttggtatat tcttttttga gcgtattgga acttagcaac
    29761 agcatcctct ctgtttccag ctgcaacagc atcttcgaat cttcttattg cagtttttat
    29821 ttgagatttt ctaactctat ttaaagcagt tttcttttcg ataacactta ttcttttctt
    29881 agctgatttt atatttgcca ccgtgttcac ctccctgtat ttttttatca tattcatcgt
    29941 caacatatta gattttaaca gaaataaatc taaaaatcaa ggattattat ttatatattt
    30001 taataatgtt ttttattttg atttt
//
LOCUS       DAEORZ010000036        29588 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEORZ010000036 DAEORZ010000000
VERSION     DAEORZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29588
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.036"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(81..1157)
                     /locus_tag="KRH39_001491"
     CDS             complement(81..1157)
                     /locus_tag="KRH39_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG8511406.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(1530..2045)
                     /locus_tag="KRH39_001492"
     CDS             complement(1530..2045)
                     /locus_tag="KRH39_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBG8511407.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(2408..3478)
                     /gene="leuB"
                     /locus_tag="KRH39_001493"
     CDS             complement(2408..3478)
                     /gene="leuB"
                     /locus_tag="KRH39_001493"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBG8511408.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDEIGEPLPNKTIENCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(3485..3976)
                     /gene="leuD"
                     /locus_tag="KRH39_001494"
     CDS             complement(3485..3976)
                     /gene="leuD"
                     /locus_tag="KRH39_001494"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBG8511409.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(3991..5268)
                     /gene="leuC"
                     /locus_tag="KRH39_001495"
     CDS             complement(3991..5268)
                     /gene="leuC"
                     /locus_tag="KRH39_001495"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBG8511410.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(5225..6967)
                     /gene="leuA"
                     /locus_tag="KRH39_001496"
     CDS             complement(5225..6967)
                     /gene="leuA"
                     /locus_tag="KRH39_001496"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBG8511411.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(7091..7337)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7485..7931)
                     /locus_tag="KRH39_001497"
     CDS             complement(7485..7931)
                     /locus_tag="KRH39_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511412.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTNESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(7952..8671)
                     /locus_tag="KRH39_001498"
     CDS             complement(7952..8671)
                     /locus_tag="KRH39_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG8511413.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(8789..10063)
                     /locus_tag="KRH39_001499"
     CDS             complement(8789..10063)
                     /locus_tag="KRH39_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBG8511414.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(10263..11630)
                     /locus_tag="KRH39_001500"
     CDS             complement(10263..11630)
                     /locus_tag="KRH39_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8511415.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(11880..12344)
                     /locus_tag="KRH39_001501"
     CDS             complement(11880..12344)
                     /locus_tag="KRH39_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBG8511416.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(12349..12978)
                     /locus_tag="KRH39_001502"
     CDS             complement(12349..12978)
                     /locus_tag="KRH39_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG8511417.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNKFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(13012..13899)
                     /locus_tag="KRH39_001503"
     CDS             complement(13012..13899)
                     /locus_tag="KRH39_001503"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBG8511418.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(13928..15166)
                     /locus_tag="KRH39_001504"
     CDS             complement(13928..15166)
                     /locus_tag="KRH39_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511419.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(15550..16692)
                     /locus_tag="KRH39_001505"
     CDS             complement(15550..16692)
                     /locus_tag="KRH39_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG8511420.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(16833..17699)
                     /locus_tag="KRH39_001506"
     CDS             complement(16833..17699)
                     /locus_tag="KRH39_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG8511421.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(18033..19580)
                     /locus_tag="KRH39_001507"
     CDS             complement(18033..19580)
                     /locus_tag="KRH39_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG8511422.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMRHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(19573..20709)
                     /locus_tag="KRH39_001508"
     CDS             complement(19573..20709)
                     /locus_tag="KRH39_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8511423.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(20972..22069)
                     /gene="dinB"
                     /locus_tag="KRH39_001509"
     CDS             complement(20972..22069)
                     /gene="dinB"
                     /locus_tag="KRH39_001509"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBG8511424.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(22459..23964)
                     /locus_tag="KRH39_001510"
     CDS             complement(22459..23964)
                     /locus_tag="KRH39_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBG8511425.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            complement(24225..24575)
                     /locus_tag="KRH39_001511"
     CDS             complement(24225..24575)
                     /locus_tag="KRH39_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511426.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(24798..24989)
                     /locus_tag="KRH39_001512"
     CDS             complement(24798..24989)
                     /locus_tag="KRH39_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511427.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(25107..26021)
                     /locus_tag="KRH39_001513"
     CDS             complement(25107..26021)
                     /locus_tag="KRH39_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8511428.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNDVSQNNIQSSTKNTAFKTTILSTGKSDCILVEIGNKVIMIDTGEDKNGKQIVDRL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEE
                     RLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGVLKIL
                     ERFNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            complement(26155..27651)
                     /locus_tag="KRH39_001514"
     CDS             complement(26155..27651)
                     /locus_tag="KRH39_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG8511429.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            complement(27931..29421)
                     /locus_tag="KRH39_001515"
     CDS             complement(27931..29421)
                     /locus_tag="KRH39_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG8511430.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
BASE COUNT         9613 a         5374 c         3077 g        11524 t
ORIGIN      
        1 aaatttaata tgtaattttt ataaaatatt atttattata tattctaaat ttatcttaaa
       61 acatacatat ttttacaata ttatttatta aaacctaata tatcatcaat attatctaat
      121 aattcttgta catcttcgac agtatagtca cccatatgtg aaattctaaa agttttttct
      181 tttaaatctc catagccatt tgctatttgg aatcctcgtt cttgtaattt tgaatttaaa
      241 tttgatacac taataccttg agtattttct ataacagtaa gtgtattgga taagtgattt
      301 tcattggtaa aaatttggaa gtgtttttta gcccagtttc taactaagtt tgccatgtct
      361 tcatgtctgt tgaatctgtt atctaggcct tcatctaata tattatctaa ttggaaatct
      421 aaagcgaaca tgtgagaaag agatggtgtt gaagggtatt ggtagttttt cttgattaag
      481 tattcataca ttgctaacaa atcaagatat actcctctaa aaggtacttg cttagctcta
      541 tcgattgctt tttgagaaaa tgtgcaaata gacattcctg gtggtaatcc cagtgctttt
      601 tgtactgatg ttatacaaat atctattcca atcttatcaa cctcgacttt tattccaccg
      661 gctgaactta cagtatctac acagtagatt acatcttcat actttttaac aacttctcca
      721 atttcttcta ttggatttct tattccagtt gaagtttcat tgtgagttac agttattaaa
      781 tcgtattctc cggtagaaag caccttatca accatttctg gcgttgttgc ctctcctaat
      841 tcaactttaa atatatccgc tgggacatta ttagccactg ccattttgta ccatctatca
      901 ccaaaggatc cacaggaaaa tactgctgcc tttttggatg tacaggaacg aatggagcct
      961 tccattaaac cagttccgga tgacgtggat aaaagtatcg tgttgtttgt atagaatagt
     1021 ttttgcatct tttcacttat actcttttgg agcatagatg catcttttcc tctatgtcct
     1081 atcattggtg tagccatctt ttgcaagact tcttctttaa catcaatagg accaggaata
     1141 aataatttct tactcattat aattcccccc tttttagttt gtaatatgga tagcgttttt
     1201 actgtcctta tttttaataa ctataatatc aagctgaatt aactatgtca acataaaatt
     1261 tattttttat atataattta ttttatatat tttatgaaaa cattcatttt ctaccttttc
     1321 ataaatctga attttctgaa ttattctctc aaaaaaaata tactttttat ctatataaat
     1381 taattttttt agtatgtaaa tttgatgcta ttacgataaa tctttttagt ataactaatt
     1441 tatttttatc ttgtattttt attgtatttt tgaattttat tttttctaaa tataaaattt
     1501 atgttttaaa atgtaaaatt tttatttaat caaagttcaa tatattccac tttttctttc
     1561 gattcttcaa aaaaccattc ttcattagaa aaatcacctt ctgcaatttg tccaataggc
     1621 attttcatat actttccagt tgctgtcact gctatatctc catttggtag aattatttct
     1681 cctgtacctt caaataattt tctgttttct ttagttattc ttccaacaac ttttatagta
     1741 gcatctgttg gtactggttt cttatatttc aactctaaag atactgttac tccccatatt
     1801 tggtcatcat ttatacttac agctcttcca atagtctcat ccaatattgc agcagacata
     1861 cctccatgaa ctctcccagg ataactttga tgccaatcct ttgtattaca aattgataca
     1921 agctctccat tttcaagttc ataaaaatct gctttaagtc ctagctcatt ttgtgttcca
     1981 caaactaaac aagatttact actattttgc ttcttcaaaa ctttatactt tgccaaaaaa
     2041 tccatctcct aaccgacatt agtagtcgct taatattaaa taattctaaa atatattata
     2101 taatattaat aaaatccctg caaacttcta ataataaaaa taggctactt caatatgata
     2161 agtttaattt aaagtattat tttccaaaaa ataactttat caaaatagaa actcaaagta
     2221 gatataagcc tctttttcgt tattaaaata gtaaaaatat attttactta atataaaaaa
     2281 gagtgtctca ttaactaata aagtttattt tgagacaccc ctcataaaac aaattatact
     2341 acaatagatt tttactaatt ctacaagtta tatattcaaa ttatattatt caaattaaac
     2401 tataattcta aatttcattt actattaatt cgcccattgc tcttgttcct acaacatttc
     2461 ccacaccatt atagatatca atagttctat aacctttatt gagtacactt tgtacagcat
     2521 cttcaataca cttagcttct tcttctaagt taaaactatg tcttaacatc atgcttacag
     2581 atagaatagt cgcaattgga tttacaatat cttttccagc tatatcaggt gcacttccat
     2641 gtattggttc atacatacca aaactatctt ctctaagtga tgctgatggt aacataccta
     2701 tagaacccgt aatcatagat gcttcatcag atattatatc tccaaatata ttattagtca
     2761 ctattacatc aaattggcta gggtctttta caacttgcat agcagcatta tcaacataca
     2821 tatgtgacag ttctacatct tcaaactctt tagctaagtc ttccattata cttctccaaa
     2881 gtcttgaact ctcaagcaca tttgccttat ctacacttat aagtttttta tttctatttc
     2941 tggctatttc aaaagctctc ttaccaattc ttcttatttc attttcgtta tatatttcta
     3001 catcataagc attttcgata ccatcaatga tttttcttcc acgttctcca aaataaatgc
     3061 cccctgttaa ttctcttaca acaacaaaat caatgccttt ctcaactata tccgttctaa
     3121 gtggtgaagc ttcttttata ctttcataca ttgtagctgg tcttaaattt gcaaataaac
     3181 ccaatgactc tcttaatttt aaaagaccag actctggtct tttatctccc ttacatttat
     3241 cccattttgg tcctccaaca gcaccaagca atacagcttc gttttttcga caattttcaa
     3301 tagttttatt aggtagaggt tctccaattt catcaatcgc gcatcctcca gctaatacat
     3361 attcacaatc gaatctatga ttaaatttac aacaaatttt atttagaacc tttatacctt
     3421 catctataat ttccggtcca acaccatcac cttttatgac tgctatatta caattcatac
     3481 tccactaccc cctatttgct tttattgagt taattaagcc ttcatttaat ataatatttt
     3541 gcataaactc aggaaatggt tctgctttat attcttttcc ttttgttata ttttttatca
     3601 cacctgtaga aaaatctact tctatattat ccccttcatc tatgttattg gcagcttctt
     3661 cacattcaag tataggaagc cctatattta tagagtttct aaaaaatatt ctagcaaaag
     3721 tagaagcaat cacacaggat actccacttt cttttattac aattggtgca tgttctcttg
     3781 atgaaccaca accaaagttt ttagttgcaa ctataatatc tccttttttt accttagaaa
     3841 taaatttatc atcaatatcc tccatacaat gcgttgctag ttctttataa tcagctatat
     3901 ttaaatatct agctggtatt attacatctg tatctatatt gtcaccgaat ttaaaaacac
     3961 ttccattggc aatcataaat tttacctccc ttaaattatt tctgatggtt ttgatatttt
     4021 tccagttata gctgatgctg ctgctacagc tgggcttgca agatacacct ctgaatcaac
     4081 atgccccatt cttcctacga aattcctatt tgttgttgaa atggattttt ctcctgctgc
     4141 aagtataccc atatgtcccc ctagacatgg accacaagtt ggtgtagaaa ctgctgctcc
     4201 tgcctctata aaagtggtaa tatacccttt ttctatagct tcaagatata ctttttgagt
     4261 tccaggaaat atcataactc taactccatc tgctactttt ttgcctttca ttatctcagc
     4321 tactacttca aggtctgata ttcttccatt tgtacaagag cctattacaa cttggtctat
     4381 ctttatatcc ttatctatct catctactgt tctagtattt tcaggaagat gaggaaaggc
     4441 tactgtttct tttagttcag ctaaatctat ttcgtatact tcatcataga ctgcatcttc
     4501 atctgcttca tatatattta catcttttgt catagttgaa cacttatgtg aatttatata
     4561 ttccatagtc ttatcgtcta ctggaaaaat tccatttttt gctcctgctt ctattgccat
     4621 attacaaata gtaaatctat catccataga taagtattct actccatctc cacaaaattc
     4681 cattgacttg taaagtgctc catcaacacc tatctcccct attatatgaa gtattatatc
     4741 tttaccactt atccatttat ttggtttatt tttcaaaaca aattttattg ctccaggaac
     4801 cttaaaccaa cattttcctg tagccatgcc tgctcccata tcagttgaac ctacaccagt
     4861 agaaaatgct cctaatgctc catatgtgca agtatgagag tcagcaccta taattacatc
     4921 tccagcagtt actatacctt tttcaggaag aagaacatgc tctattccca tatctcctac
     4981 atcaaaaaaa tttacaactc cttttgattt tgcgaaactt cttgtaaatt tacattgttc
     5041 tgcactcttt atatctttat taggtgtaaa gtggtctaaa accatcacta ctttagtctt
     5101 atcataaaca tcttcaattc ctaatttttc aaattctcta atggctactg gtgatgttat
     5161 atcattacct aaaactacat ctaaatctgc ttcgattaat tgccctttct taacttcatt
     5221 taatttagca tgtttggcta atattttctg cgtcatagtc atccccattt tttatttcct
     5281 cctcaatata acttttattt attgcattca taagtgcatt aatagctgct gtatttatgt
     5341 tttccgatat acttacacca aaatagactt cattattgtg ctctattcct acataagcta
     5401 cccctcttga atgtgaccct tcctctaatg catgttccca gtaatatcta aatttataat
     5461 ctttcatgcc acattgcttt agtgcattat taaatgaatc tacaggtcca tttcctatac
     5521 caacaatcct ttgttcattt cccatataac ttattcttgc agtcatctca atcatgttag
     5581 tatcattaga atcatcattt tcatagttta attcatccat tgattttatt ttatacttct
     5641 taactttata agggctctca acttcaatat attctttctt aaacaaatta aatattgctt
     5701 cactactaag ttcttcccca attctatcag attccatttt gactacattt ccaaaatcag
     5761 aatgcatatt cttaggtatc ataaatccat aatctgtttc cattatataa acagctcctc
     5821 cttttccaga ttggctattt atccttataa tttctttgta ttctcttcca atatcatgag
     5881 ggtctattgg taaatatgga acttcccagt aatcattatc tctgtttttc attgctatca
     5941 tgccttttct aatagcatct tgatgtgaac ctgaaaaagc acaatgtaca agattacctg
     6001 catatggatg tctatcatgt accataagtt ttgtacagtc ttcatatata cctattatct
     6061 tatctatatt gctaaaatct agctctgggt ctattccttg tgtataaaga ttaagtccca
     6121 catttactat atccatattc ccagttcttt caccatttcc aaatagtgtt ccttcaagtc
     6181 tatcggcacc agccaataat ccaagttcac ttgcagccgt acaagttcct ctatcattgt
     6241 gagtatgcaa acttagtata atagaatccc tacaacttat atttttacaa aaccattcta
     6301 tttggtctgc atatacgttt ggtgttgcca tttccactgt tgatggaaga tttattatta
     6361 cttttttttg aggagttggt ttccaaacat ctatcactgc ctcacatatt tcaagtgcat
     6421 aatccatctc tgttcctgta aaactctctg gtgaatattc aaaagtaaat tctgtttctg
     6481 gatatttttt tacttcttca ttaaataatt ttgctccttc aactgctata tctattattt
     6541 cttgtttact catattaaaa accacatctc tttgaagaac agatgtagag ttatataaat
     6601 gtataattgc ttttttacat ccccttattg attcaaatgt tttttctata agatgagccc
     6661 tagattgtgt aagtacttgt atagttacat catctggaat catattctca tctattaatt
     6721 ttcttaaaaa tgtatactct gtatttgatg ctgatggaaa tcctacctct atttctttaa
     6781 atcctgtatc aataagcatt ttgaacattc tgactttttc atttacactc ataggtgttg
     6841 gcagtgactg atttccgtct cttaaatcga cactacacca aattggagcc ttaaatattt
     6901 cattatctgg ccatttacga tttactattt gcattttatc atatttttta tactttccac
     6961 acttcatgtt atttccccct ttttctgttt tttgtattca gtatacctca tatcgacgca
     7021 atcctgcaca attcataagg ataaatgtca attttatcat tagaataaaa aaaacctcat
     7081 ctctaataaa attagagacg aggttttgct cgcggtacca ctctatttag ctttctaaaa
     7141 agctccctta gtaatagcca atacaataat tataatgact atatttccta taacgtggaa
     7201 ttactccgat tattctaaga tttaaatctt agaaaacagc tcctagacaa gttcagaact
     7261 atcaactact aaatcacacc atcctttagc tctctgaaag tttcattgtt cttactactt
     7321 ctattcatca cttttttaat catttaaatt ttctaaatat tttgatttaa tagttattat
     7381 atacacatta aaatgtattg tcaacacaaa aaataattat ctcaattttt tatttttgat
     7441 aagcaattct ataaaacatg ctaactattg aaaacagtat gatattatgc cttaaacttt
     7501 ttcatctttt catctatgat attcaataat tttttttcat cttccaaact caatgccctt
     7561 aatctaattt tataattaga ttcgttagtg tatatattta atatattttt tactacttct
     7621 attttttcta ttatcttata attaaacttt tgactttgtt tagattttat actgaatata
     7681 cattgattat ttattaatat tgaatgatta tcgagagtaa tatatacttt tccctcatat
     7741 ttatttgaaa aatcacgacc ttgtttaaat agaaaaactg ctgttattat acataaaata
     7801 tataatattg tcgttgaata tataaaaatt gtagctaaaa ttataagtgc taaaaccata
     7861 tatatagtca ttcctttttt aaaataacca ttgtcataat aattcatata gataaactta
     7921 tttttactca aattttttca ctcccaagat tctaaaaatt tatatcaaaa aattctataa
     7981 tttcatcttc atgataattt atgactaatt catctggaaa atctactgct tctaaaactt
     8041 tttctgcatt ttcaaattct cctatagaat aacatatatg agagtcagta ccaagaatta
     8101 ctcttgcacc atatgtttta cacaatgtca acatatgact tacattttcc caagtacccg
     8161 ttctatgaga gtttgaactt aatgatgaat tatttatttc taaaaggata tttttatctt
     8221 tagctttttt tactataggt tcataatcta aaggatacct actgtcatct ggatgtccaa
     8281 ttatttttac cttaggttta tccataactt ttaaaatagc atttgtattt tcttcttttg
     8341 taccagaagc tatacaaggt ctatgcaaac ttgcgattat ataatccaaa tgttgcaaca
     8401 tatcttcttg aacatctaaa ttaccattat aatccattat atttccttct attcccctaa
     8461 gaatctttac accttgcact tcacgtggaa gcaaatgcaa attataaaaa tagaatggat
     8521 gtggtcctcc tggcatatta ggtccgtggt ctgatattcc taagtatttt atattcttct
     8581 tactcgcctc ttcgacattt tccttgaaag tactatgtgc atgtccactt attagagtgt
     8641 gacaatgtac atccataata gactttttca ttaaatttcc tcctaatatt ttacataaat
     8701 tcgtgaaaat agctaaaggg ctatttcaaa actaaagctg tcataataga cagtgtaacg
     8761 ttttaaaaca gccccatttt caattatttt acacttctaa atctttaaat ttatctttaa
     8821 ataattctcc aatacttcct aaagattcct ctacttctag gtattctgtt atatcctctt
     8881 ctggctctct agttgcttct tttattgaaa ccaacattct tttagtcttt ggtttaaaat
     8941 caagaatctt aactttaacc tcatctccaa tattaacaac attagtaact ttagccactc
     9001 tagtatcact caattcagat attggtaaat acgcatctac accttcttta atttgaacaa
     9061 aagccccttt ttctattatt ctaagaactt tagcattgac aacatcatca atttgtactt
     9121 cttttgctat taaatcccaa gggtcattat ttatgtcttt taatgccaag gcaatttttt
     9181 tagtctcttt atttacatcc aacacataaa cttctacttc ttgtccctct gaaacaacat
     9241 cttcaacact ttcaactctt gtccaagcta aattatttat atgtgctaat ccttcaatcc
     9301 caccaatatc cacaaatgca ccataaggca ttatctttgt caccttacca tctctttttt
     9361 gtccaacttc aagagaatta aataagtctt ctttagctgc tttgatacga gctttttctt
     9421 cttctctttc ttgcttaatt cttgctcttt cagcttcttt ttctgcctta atttgagctc
     9481 ttctttcttc tctctcttta tttatacgct cttgtaatac atctctatga gatgctacaa
     9541 gtctattttt tctaacatcc aattctttta tataaacttc taagttaaga ccaacaaatt
     9601 tagatgtatc tgtaataaat tttgtatcaa gttgagaaat tggtataaaa aatgtatatg
     9661 tattgtaatt agcaaataca cctttatcta tattcttttc aacatttaca gttattattc
     9721 tatgttcatc ataagccttt tgtaactcgg caaaatcatt tcttttgtca agttgtaatt
     9781 tagataaagt tattgtacca tctttttgag atacttttgt tattacagca gttatttcat
     9841 cacctatgtg gtatatatct tctatatatt gatttttttc tatgttcaat tcacttatag
     9901 gtattattcc atcaaaccca tagtctaatc ctaattgtac ttcatcattt ctcactgcag
     9961 ttatttttcc agttgtcaat tctccaactt tcactttgct gaatacttgt tcttgctggt
    10021 caagtaattc ttgcattgtt aaatcatttt ccattataaa catcctttcc tcttattgat
    10081 tatcatatct gtaaatatta taccattaat attgcaaata cttacatttt tcttactata
    10141 atttttaaaa taaattcaac ttcatatatt tatacattat agaaaatatc ttattaaatt
    10201 aaaatacaaa ttctaatttt tatctaataa aatattttca taaaaaattt atattgtata
    10261 tcttattctt ttattgcttt aggttttgat agaaaacatt tataaaatat aaagaaagaa
    10321 attgctgtta tagtagcagc tgttatatct gcaactggct ctgctaaaat tactgcaaaa
    10381 agtttttcat taaagaatgt tggaagtata aatattaaag gtattaaaag tacaatcttt
    10441 ctaagtagag ctaaaaccaa agatattgtt gcttttccaa gtgctaaaaa tgtctgttga
    10501 caagctattt gaatcccaaa cataaaagca ccagcaaaat atatttttat actccaacta
    10561 gtcatactaa gtaactgcgg attcttatta aatatactaa caaaaaatgc aggaaatatc
    10621 attaataaaa gccataaaat agtagaatat gtaaaacatg acaataaaca caacttaaat
    10681 gtctttttta ctctatcaag ttgacctgaa ccataattat aacttattat tggttgtgct
    10741 ccttgtgtaa cccccatatt aggaagcata agaatttgca ttatactgct cattatggcc
    10801 attgaaccta ctgctaaatc tcctccatac atttgtaatt tactatttaa ggctataagt
    10861 actaaacttt ctgtagattg cattataaat ggagagattc ccaagccaag cactggtaag
    10921 atgatacttg cttttggtat catatacttc ttttttattt taagtatact tttctttcca
    10981 aagagaaaca taagtaccca aatagcagaa acgccttgtg caattatagt agctaatgca
    11041 gctcctttta cacccatatt aaagccaaat ataaaaatcg ggtcaagtat tatgtttatt
    11101 aaagcaccta tagcaactgt catcattcca gttgtagcaa acccttgggt atttataaaa
    11161 gtattcatac ccattgaaat ctgaacaaac actgtaccta ttaaataaat atttaaatag
    11221 tccattgaat atccaattgt agattcactt gctccaaaca tccaaagtaa aggctctttt
    11281 ccaatcttaa aacctatagt caaaagtatt gctaaaatca ccagtacaga aaaactgtta
    11341 ctcattattt tctctgcctc atcattgttt tgctcaccca tttttatagc agctaatgga
    11401 cctcctccca taccgattaa ataactacat gcagttatta taattattat tggaaatgcc
    11461 actccaacac cagccatagc aacttctcca ttttccattc taccaataaa aatcctatcg
    11521 actatgttgt ataaaacatt tacaatttgt gcgataatag caggtgtagc aagtttaaat
    11581 aataatttac ccacactttc actacctaag ttagtgtttg aattttccat tttaaaacct
    11641 cctcaatcca ataattaaaa tgtatcacta aaattttatt ctatataaaa atttcatttt
    11701 attagcagac aaatttagag tactttccct acaagtaata ctatactata tttcttttgc
    11761 catagtaaac attttagaac taactgaagt taaatacttt atctcatttt ttatttatta
    11821 ttaaaaattt ttcacacata ctcataaaaa aactatccaa gtatattact tagatagttt
    11881 tatattatat gtaaagaaag agctgaaact ataacccctg ataacaaatt gccaagaaat
    11941 actcccataa aagcatcttt tattctcatt tttaatatgg aagctatagc agatgcagtc
    12001 catgttcctg tagttggcag tggtatacct acaaatagta ttataccaat tatcgaaaca
    12061 ctttttaatt tcttcattct tgagtttatt tttctatcaa ttacatcagc aatcccttta
    12121 aatagtttct tccctcttaa aaattgtaat acatgtctga atgtcaatat caagggaatc
    12181 gataccaatg tagaacctat tacactaaat acataaaccc atataggatt taagcccatt
    12241 gcaataccta taggaatggc tcccctcaat tctgttacag gaaccataga taaaattaat
    12301 atgtgtaaat atcccaaact attccctcct attctaagtg acattaagtt atgaatttat
    12361 ttgctctata acctcaccat cctttttact aactaaaaat gcatttttag tcaatgaaag
    12421 cattggtaaa tcactcttag agtctgaata tgcatatgaa ttttcataat caatctctat
    12481 cccaagttta cctaatacag cctttattct ttttactttt tcaattccct tacagttttc
    12541 tccaatgaat ttatttttat atcttgaatt ttcataaaaa aactccgtgc ctataacctc
    12601 gtctgcaaga ccataatact taaaatattt catatatgca tatggagatg cagttatcat
    12661 aattataaca ttatcttctt ctttattttt gtaaataacc tttttcatag attcgttaat
    12721 ttttttcatt atgcaatcat caaaaaactt ttttaaatct tcttcactaa aatctactat
    12781 tggataaaag attgcctctt ttgccttctt caaaggtatc attctaatta aatacataaa
    12841 tagaataaaa ataatgtatg gtaactttat aatagtttta aaggttttat tttttaaaga
    12901 gtaaataaaa aaatctatca tgctatcagt atttataaca gtataatcta catcaagtaa
    12961 taatacactt tttttcataa gtatttcccc atcctatcac taaaaatatc actaaaaaat
    13021 caatattatt aaacatgtaa ttatccataa gattacgttt atttttaaag cattatcttt
    13081 taggacaata tcagtaggat tattttcatt tgtagtgtta attatataat tatatctaag
    13141 tacaccataa actacaaata ttgttgtaaa tatcatatgt ggcttttctg gattacttgt
    13201 agaatacaat gcataacata caattgtaga acttaatata actatcatca tttgatttag
    13261 attttctatg tcataatctt ccaatatttt tctatgttca actgcatcat ctcttagagt
    13321 ttctatttct tttttacgtt ttccaagagc taagtacaga gataagaaaa atgtacataa
    13381 aattatccaa tttgataaac taacacttat ggctacacat cctgcataaa ccctaagtat
    13441 aaaaccaaga gatattgaaa acacatctaa tagtattata ttctttagtt taagagaata
    13501 taatatattg tttatcaaat agagactaag aattatagaa agattaaaac taacaactgt
    13561 tgataaacag actgaaatta aagctaatac tacaaatagt atattagcac tttttttgct
    13621 tattcttcca cttgccactg gtctattaca ttttttagga tgttttttat ctttatccat
    13681 gtctatagca tcattcagtg catatactga tgatgacatt aggcaaaaac acacaaacgc
    13741 aataaacgta tttaaaaata aatttatatt tagtatgttg tttgaaaaaa ttatagctga
    13801 aaatacaaag aaattcttta tatattgctt tattctcatt aactttatat aatctgaaat
    13861 tttagtaaga agccttatac ttgtattggt ctgtttcata aacttactac cctttcaata
    13921 ttatttttta aacaggatat gattttatcc ctatgtttaa aatttctata aatccattga
    13981 cattactgat tactatagtg accaataaca gcgatagaaa tatattatac atattctttt
    14041 cattttttct aagcgctttc gtaaagtaag ccaataaaat tataactgca aaactaaaat
    14101 ttatagccat aatacttgaa ttgactagat tatatcctac tataccatgt aaagttagat
    14161 tgaatataat tattcccata catagcttag gaactctttt ttctgaattt tcaataactg
    14221 ataaaacaca aaaacataaa gctaataatg taagaattac tggtattgta gcaacttctc
    14281 cattttgcaa aaataaccct tttttaatac ttgctccagg aaaataaaaa accgaataca
    14341 ataatctctc tataatcatt gcaatctttt ccaaaattgg ttctccactt ataaatttcg
    14401 tatcatactg tattacattt ccaaggttca taaaactctc ataagacttc gttgaaacta
    14461 cacaaatata tgccattagg ataaatgtaa ttaaatgttt taacattttt cctattttta
    14521 atttaaactc aaatatcata ttaaaaaaat acaaatatat attactaata gttattccaa
    14581 atagaggaat agctgctaat tctactaaat aagaattttt tcctttcatc ttatttgcaa
    14641 taactatgaa aaatattaat gaaaattggc tatatataaa tctctctaca tttaaagtca
    14701 taaataactg tggaaaagaa aatatcatta tcatagtaag taaaattaaa gttaatctct
    14761 gcatttttat ctttttaata tgattaaaga ctaaagttat tgacatagca ctagttatta
    14821 tttgtagaca aattagtcca aacccatagg cactattaaa atctgttgtc ccaggatttg
    14881 ctattgaagt acaaaatagg tatattggat atgcaaaaat tgatataatt gctgaaaaca
    14941 atatgtgctt actattgtct tgacttatag caaatacatt taaattgaat aaaactcctg
    15001 tatctgtatc taacaataca tcataagtat tgtttaaatc ataaaatata gtagtcttag
    15061 aaaaaaatat cttagttatt attaagtaag ttattacaac taatataaac aaaaacatct
    15121 cgtttttata ttttaatatt ttactcatca atatatcacc atccattcat tatataggat
    15181 ttgctgacac ctacaattta ttccttatat ttaatagcca tcttcataat gttcttactt
    15241 gttttattgt aattgaataa ctctttattt acaaccacaa tatttacttt attgatgtta
    15301 taatcagtca agtattgttt atataacata gattagtgtt gtataaaatg agaatttgta
    15361 agcaatatta atgtaaaata tttgaaaaac tttatataaa aatatacaat gttgcttatg
    15421 gaattaaact ataacaaatt aaagcataat ttaaggagac aaaattataa tctgtctcct
    15481 taaatattaa aactatattt taaaacatta ctaaatacta tacaaatttc aaatatagta
    15541 taaaattaat tagactttaa cactattagc tccatcttct tcccattttt taatttcttt
    15601 taatctatct attataggta aatgctgagt acattcaact tcacattttc cacaagatat
    15661 acattcttta gcttttgcct tactattcat tgtgtaattc caatgtccta agccataaac
    15721 taactttgtc atttcttcat cactcttttt aaacatctgc ttttcattat aaaaaagcat
    15781 ataggctggg gtattaatac cttttggaca tactttacaa taaccacaac ctgtacatat
    15841 ttcgttcata ttcttattta atctagtctc aatatcatgt atatcctcat ctgaataaat
    15901 tctattttcg tctgcaattt tacatgcatc atctatatct tgttttttat taaaccctat
    15961 caaagatata cttatttgtg aaattcctat gttgaatctt aaagctgatt ctatagtact
    16021 ttcgccatct tttgatagaa aagaaagttc tttattatac tttggaatag ttccaccact
    16081 taatggattc attgccacaa caccatatcc attatcaaca gcatatttgg caccttttag
    16141 cctatatgga aagtttaata tatttattcc catagttaca ccttcaaact tattttcact
    16201 tagaatatct ataacctcat ctcctggttg atgagatgaa aatactatat ggtctatcaa
    16261 tccttcttct ttacatttca ataatccttc atattgacct ccaggtctca ttgctagttc
    16321 gtaatgttcc atctttctaa tattccaaac atgataaaaa tttattttag atacgcctaa
    16381 tcgttctatt gattttttta cagcctctat agctttttct gatgtatcac atgcttttgg
    16441 cttttcttta gttgatacat aaaaatcggt cttaccttcc tttatcattt gtctaaaggc
    16501 tagtccaaat atatcttcac ttctgtcatc acaataccct ggtgctgtat caaagtaatt
    16561 aatacctttt tcatacgcat atttgactaa atcagcattt tcttgattac ttttttctaa
    16621 atcaaacctt aatcctccat agccaactac tgaaaccatt ttccctgtct ttccatattc
    16681 aacgtatctc attgtaaatt cctccttaga tatattaaga cttaatttct ttatagaaaa
    16741 tattatacca taaataattt ttataaatta tttatggtat aatatttttt ttataaatta
    16801 tattttttta taacttgtat tttataacta agttaaaaac tataaaaagg ttcaatatgg
    16861 actattactt gagcattttc attaatttct tcttgtattt gtttctcaat tttatgtgtt
    16921 aaatcatgag attgctctac gcttataaat ggatctacca ttacatgcat gtcaatataa
    16981 atagaacttt tgctacctct acttctgata ttgtgaacat cccttacctc attaaattca
    17041 aacacaattt cttttatata atcctcatct acagccactc tatcgaccaa aattccaata
    17101 gatggcctaa atattccata agccgaataa attatgaatg cagaaataat aataggaact
    17161 atagattcta ttattactgg aaatcctaac ttaacaccaa ctaaagtaac caatacacct
    17221 agagacacaa atatatcact tcttgtgtgc aatgaatccg aaatcaaaac ataactattt
    17281 agtttagtcc caattctata ttcatacatg catacaaaaa tatttataaa aagtgtaata
    17341 attaaagcaa ttaaactttc tatagttata gcaggtacaa ctggattagc tattcttaac
    17401 actgcactaa gaattatctt aattgctata acaaacaaca taccacttat aaataaactg
    17461 gttataattt caaacttttt atgtccataa ggatgatttt tatcttttgg tttagatgca
    17521 aagaaaatac ctataagccc cactatatta gatgctccat ctgacaaaga atgaaatccg
    17581 tctgctgtca tactatagct ttttatttga ttaccaataa tgatttttaa taatgctact
    17641 gcaaaattag caaataaaat tatccacaat acttgcttca ctttcttata gttatccata
    17701 acaaattccc cctcccttcg attcaataaa gaaagctatc tatcaatatc aatcaaccag
    17761 acaataaaat cgtatataag cacatttact atatttatat tcaaaattgg ttgtacttgt
    17821 tcattctatt tgaaacttaa ttataaataa aaatcgtagg tatggttttg gtatacataa
    17881 aaataagaga ctgtctcaaa ataaagataa tcttcttttt tattgttgaa atgataaaat
    17941 atatttgatt tcatataaaa aagagtgcct catgaactaa aaagttcatt ttgagacact
    18001 cccttaaatt tttgatataa attattaatt ttttatacta agccttcttt aactaagaat
    18061 gaatgagcaa cttcttctgg tgatttacca agttcatcca cttgatagtt aagttctctc
    18121 atagtttctt catttatttt tcctgaaagc atattcataa ctttttctaa ctcaggatat
    18181 ttcttcaaag tatcttctct tactaatggt gctgcataat atggtacaaa gaaattttta
    18241 tcgtcctcta atatcttaag attaaacttt ttaataagac catcagttaa aaatgcatct
    18301 ataacctgag attcattatt atctaaagct gtatatctta aacttccatt cattgctttt
    18361 acatctttga attttaaacc ataatctcta gaaagcccta caagaccatc ttctctattt
    18421 tcaaactcca aagttggtga gaatgtaaat tctttaccat atttagtcaa atcagaaata
    18481 gtatttattc catacttttc agcagtttca ggcatcatgg ctaatgtgta agtattatta
    18541 aatccaagag ggtcaagtaa agttaactga ttttcttttt ccatagtatc ttttacaaca
    18601 ttatatgctt catcagcatc tttaatcgga gcatgtctca ttacatttac caaaagtgta
    18661 cctgtatagt caacatacat gtctaactca ccagatttaa ttgcattaaa tgcaacagat
    18721 gaaccaccta gatttaaatt cttcttaact ttaagattcg tatttttttc tactaattca
    18781 gcataaacat tgccaagtat aagttgttct gtataatctt tagaaccaat agttattgta
    18841 tcttttttac ttgaaaaact agaactaaaa acaacgaaca acattgaaac aacaagcact
    18901 acagatatta ctttaagtgc tatataattc ttcttaggtg cttttggatt taatcctttt
    18961 ggagtaactg caacttcaat tttactaaat aaataatcaa ctaacagtgc aattatacat
    19021 gatgggattg cccctgctaa aatcatatta ttatttacag tttgaacccc tgaaaatact
    19081 aaatatccca aaccaccagc acctataaat gctgcaagag tcatcaaacc aacagctgta
    19141 actgcagata ttctaactcc acccattatt ataggaagcg ctaaaggaaa ttgtatttta
    19201 aataatgtct gatttcttgt aagaccaata cctttagctg attctaatac tactgggtca
    19261 atacttgtta tacctatata cgtatttttt acaattggta gtaatgaata aaccactacc
    19321 ataaatatgg ctggtttact tcctatacca agtataggta caagaagacc caaaagtgcc
    19381 atagacggaa ctgcttggac taagtttaca aatccaagta caaactttct aagagatgag
    19441 attcttgaaa taattattcc aagaggaaca ccaactatta tagctattaa aatagatgta
    19501 acagttagac tcatatgttg tattaaaagc tctataatct tatctttttg taatagtata
    19561 aagttaaaaa aattagccaa gaacactcac ctccatttct aagaattgtt cacttaaaac
    19621 agaaagtaaa ctacttctag taataagccc taccagttta ttttcatcag aaataaccgg
    19681 aatatatcca accgaattac gattcattac atttagtatt tctactaaat tatcaccttc
    19741 atttacatgc aaaggctcac ttgacataat atcagcaagt aatattgact tatcatcaat
    19801 atctttcata tcttttacag taacaatacc ttttaatgtt tttgctctat ctataattag
    19861 taagctatca accttactcg ttctcataat ttctattcct tgagttacag ttctagtaga
    19921 attaacagca ataggatttt ttatcataat atctcttgct tttatatatt ctggattatc
    19981 ccaaactcta tcttctccga taaaatcttt tacaaagtca tttgcaggct ttctaagtat
    20041 attttcggga gtatcatatt gtgctattct tccatccttc ataatacaaa ttttatcagc
    20101 tatctttaaa gcctcatcca tatcatgagt tacaaatatt atagtttttt tcaattcatc
    20161 ttgaagagaa aataattgtt cttgtaaaga tgttctcgtt attgggtcta aagcactaaa
    20221 aggctcatcc atcaatatta tttcagcatc agtagcaatg gctcttgcaa caccgattct
    20281 ttgttgctgt ccaccactaa gttctgaagg atacttattc aaaaattcat cactatcaag
    20341 tcctaccatc tcaagtagtt gcaacgttct tttttcaatt tcttcaacgg atttttcctt
    20401 ttttaggcgt ggaattagct ctatattttc ttttattgta agatgaggga ataggcctgt
    20461 gttctgtata acatatccta tttttcttct aagctctatt tcattttcct gagaaattgg
    20521 tttgccatta atatatatct caccagatgt aggttttatt aatttgttta taagcttcaa
    20581 agtagtagtc tttccacaac cacttgaacc aatcaaaact accaatgtac ctgtttctac
    20641 aactagagaa atatcatcta atataacatt atttcctatt ttttttgtta catttcttat
    20701 ctctatcata atttatcatc ccctttttcg aacaagcaac cttcttcaat attataagtt
    20761 gtttttataa ttatatcata ctattttatt tatgtaaaca aagtacgatt tttaggtcac
    20821 tatttgtctg aatattgcta caaatataaa gtatttatat atagtatata atatataatg
    20881 tcaaaaaaca aaaaatataa aaatattcat aaaataacac tagcctcaat tatatagtat
    20941 taaagctagt gttatttcat ttaaacaaaa ctcacatttg gatactatcc atgtattcaa
    21001 ataatgtaat atctttataa gtttcttttt tatcacttag gtttgatatt gtaactccca
    21061 atagcctaat ttgttttcca ttaacctcaa catttctcaa caaattatct acaccacttc
    21121 ttatatcatt atattcgtca atatagtgtt ctaaactaag gcttctagtt atctttgtaa
    21181 agtcctcata tttaattttt aatgttaatg tttttccaaa cttctcagag ttttttaatc
    21241 tatgacatac ttcttcacaa agttcatcaa gaatgttgcg tgtttcttct tcatcaatat
    21301 ccaaattatg acttaatgta gtctcagccc caacagattt tctaacccta tttggttcca
    21361 ctggtctatc gtctattcct ctagcaaatt tatataattc ataacctctt tttttgaata
    21421 tattttcaag ttcaaataaa tttaaacacc ttaaatcata tccagttttt atacctaaat
    21481 ttttaagagt atttgaagta acttttccaa caccaaaaaa tttattcaca ggtaatttat
    21541 ctaaaaagtc ctgggcattt tcttcggtta ttacagttaa cccatttggc tttcttaagt
    21601 ctgatgcaat cttagcaaga aacttattgt atgacacacc agctgaagaa gtaagaccaa
    21661 cttctctaaa tatatctttt tttatttgct ttgctatctc aattgatgaa tcaatatttt
    21721 ttttattttt tgttacatct aagtaagctt catctaaaga taatggttca actaaatccg
    21781 tgtatctata aaatatatct cttatttgct ctgacacact cttataaaca tcaaatcgag
    21841 gtcttacaaa aactgcatat ggacacctat tgtaagctac agctgatggc atagctgaat
    21901 ggataccaaa ttttctagct tcataagaac acgttgcaac cacacctcgg ctttgtggat
    21961 ttccaccgac aataacaggt cttcctttta attttttatt atctctttgt tctatagatg
    22021 cataaaaagc gtccatatct atatgaatta tttttctata atgtttcatt attatcacct
    22081 tttaaagtat aaatagatta attttattta tattactttt taattatacc aaacttatgt
    22141 ttctttttgg tattttttca ttattttctt tatatattca tattttccat aaataaaacc
    22201 atctataatc cattaacttc aatataattt ataaaaaatc cttaaaaaca cttgcaaaaa
    22261 ctatataaaa gctttacaat aggtattcca aggaactaaa tttaaaattc cttatggata
    22321 acagtatata tatttaattt atgggatgtt ttaaaaacaa aagactgtct tatgcaagac
    22381 agcctactga ttttattata attgtaatgg actgctcaaa ctattacttt aaaacagccc
    22441 atattttttt atataatttt aactatagta aagattttgt gaaggatatt ctgggtcaca
    22501 agttacatct ataccaagct ttctaaatgt ttgttcatca tctctattta atatagtagt
    22561 agaatgagct tgacaccctt ttaataatgc taacttttcc atagcaaatt gtgcagttgg
    22621 attagttaca gcacaaatac taagtgcagt taacacctct tggcaactaa gtgaaacatt
    22681 tctactagct aaagtctttg tttttaagtt tatgataggt tctaatatta caggtgatat
    22741 taagtgcata tcatcagata tattggctaa atgtttaatg gcatttaata ctactgctgc
    22801 agttccattc attaaatcag aacccttacc agttaatatt gtaccatctt ctaattctaa
    22861 tgcaactaca ggacaagtat cattcttgtc agcactttct ttcaatttat tactgtagtt
    22921 tctagcaggt aatacaacat ttctatcttc tggctttaag ttaagctctt ccataatcat
    22981 tttcattcta tcataagcac ctttatcaac ttgtcccttt ttatactcac aggcagtttt
    23041 aaagtatctt cttattattt cttctataga agcttttctt attacttcat catctattat
    23101 tccaaagcca actctattaa cacccatatc agttggagat tggaaaattg attcttttcc
    23161 agttatcttt tctattattt ttttaagcac tggaaataat tccaaatctc tattgtagtt
    23221 tactgacatt tcaccatacg tttcaaggtg gaatgaatct atcatattca catcttttaa
    23281 atcaactgta gctgcttcat aagctatatt caaaggatgt tttacaggta cattccaaac
    23341 tgggaatgtc tcaaacttag aatatccaac tacattacct cttttatttt catgatacaa
    23401 ctgacttaga caagtaccaa gttttccact attaggtcct ggtgctgtga ctactactat
    23461 tggctttgta gtttctatat attcatttgc tccatatccc tcatcactaa ctatagtctc
    23521 aacatctgac ggatatccct ttgttggata atgcttataa actttaattc ctctacgttc
    23581 taatttattt ataaatacag ttgtagcagg ttgtccttca aatctagtaa ttactacact
    23641 atttacatct agttcataac ctcttaaatc atctattaat cttaaaactt ccatatcata
    23701 agtgatacca aagtctcctc ttattttgtt tctttctata tctcctgcat agacacaaat
    23761 tacaacttca actttttctt tcatatgttg taatactttt atctttgcat tttcatcaaa
    23821 tcctggaagt actctttttg catgaaggtc tcccatcaat tttcctccaa actctaaata
    23881 aagtttgtca aaattattta ctctttctaa gatatattta gactgctctt ctaaatattt
    23941 tttgtggtca aatcctactt tcatcttaac acctcactaa attaaatttc ttgttgataa
    24001 cagtatattt atatatattt ttttagtaaa acatcttata aaaaaatgtt aatacttttt
    24061 cactcaaagt aatattatac aattaagatt aatttttttc aagatagttt tatggtaaat
    24121 aagatatacc tatataacta ataaaataag aatattatat ttttatatat aagtataata
    24181 ttctcacctt agcattaatt tatttcaata ttttcatata tcttttattc taaattcaat
    24241 acttttctaa tatcttctat accttgataa acttcttgtt tagaaaaatt catagcttct
    24301 aattcttcat cggtcttttc cataagcata gtataaaatt ctattgctat atactgcata
    24361 ttttcactat ttacactctt agctttatca aataaaattt cttctgcttc gtttacattt
    24421 ccctgaaaaa ctaatctttt taaagttata agtataactt cttcttctgt attatacact
    24481 aaatcttttt cttcatattc actgtatttt tttcccaata aaagtcttgc aaccataagt
    24541 tttatctcat tgaaaatctt atccactata gccattttta catctcctat aaataaattt
    24601 tattttcttt ttaattatat cagactttat cgtaaaaatt attatatgta aatgaaataa
    24661 ttcaaaaata aattttaaca ataaaaaaaa gaaggtagcg ggggatttcc gactaccttc
    24721 aaacactaga ttgtgattgg tttggtattt tatttatacc caccataagc tttattaaat
    24781 catctaaatt gattttttta gcatccacat cctccatcaa aaacgtcacc acattcacaa
    24841 agtattaata ataatattaa gaaaggtatc catgcacata agtccatttc agcccaacaa
    24901 tcttggaatg ctaagaataa gaagaataat attattatcc accaagcaca tccaccaaat
    24961 ccgccttcac caaaaaatct atccaacata atatgttacc tcctagtcat tttgtatgtg
    25021 gagtttcagc tcctaaaata tactatgttt aaggggtaat atttgctaat gattttttta
    25081 aattttttta gatattttta aaaaaattat tgattcacac tcaatgtctt accatcacta
    25141 ttgataacta cctctccatt agaagtcaaa aatgttttta tattaaatct ttccaatatt
    25201 tttaaaacac cttcctctgg ttcttttttc tctgaacatg taataacagc atattggggt
    25261 gaaatagact ctaaaaatgt ttcagttaat ttgtcatatc ttccatgatg aggcatttta
    25321 acaaaatcat attttaaagt atttccattt ataaactctg acaatctttc ttcctctgca
    25381 tctcctgcaa ataaaaactt atgtgctcca tagcttatat tagtgattat tgaataatca
    25441 tttgaactct cgtacttgct ttttgaagcc ggatgtatgt ttatttctgc actattgatt
    25501 acaatattca tattatcttt tagtagcact ggttctatat cagctttttt aagtgagtca
    25561 atatattcat catattgctt actatctttc ttataatttg cttgtataat attctttact
    25621 ttaacactag ataacacact atctactcca cctatatggt ccttatctaa atgagttaaa
    25681 attaaatagt caagtgtatt tattcctttt tcttttaacc tatccactat ttgttttcca
    25741 tttttatctt ctcctgtatc aatcattatg actttgttac ctatctcaac caatatacag
    25801 tcagactttc cagtacttaa tatagtagtc ttaaatgcag tattttttgt gctactctga
    25861 atattatttt gactcacatc atttttgtca ctttttaaat aagattcact tgttgatttt
    25921 atcttactat tagtagttat atttgttaca agcaataata ttactattat agataatgta
    25981 gtaactaaaa acaggatatc ttttaatact attttcctca tccaaaagct cctataaatt
    26041 ttatttatta catatttata gtatagcaac cttaactctg taaaatacac atataaaaag
    26101 ggcaaatttt aggcatattt gtggcataat aaaaaaatga ctgtaccaaa acaattatct
    26161 ttctaaattt acatcttcaa attctttaat cttatcatta gataaaacac tctttttagt
    26221 agattgtcca ttttttctat caaaagtatt tttagctttt acacctgata aaaacttacc
    26281 tgctccatta atacaacctt cttcacacat cattccttct ataaaattac cttctaatct
    26341 tcccaattta gccattgtca ttgtcttttt aatttgtctt ggaccagata cttttatagg
    26401 ttcaaaatca aaactaagcc ccttttcagc aatatagttc tgtactgatt tggcaagtcc
    26461 accagatgct ccaaatcccc ttccaaaaat cgaaccatca tttatatctt catttgaaca
    26521 ttttgataaa tctatattaa atgcttcaaa taatgccatt agttcttcaa aagtaagtac
    26581 ataatctact gaatctttta aacttttctc tgttgcctca ctctttttag ctgtacatgg
    26641 ccctataaat acaactttag catcagaatc tatttttttt actaatcttc ctgttgctac
    26701 cataggagac actgtagatg atattctatc tttttcatct gggaacatct tctctatata
    26761 cttaaaaaat cccggacaac atgaatttgt catgtaatta tctcctgatt ttattctttc
    26821 aataagttca ttgctttcat gaactgtaac tgcatctgct ccacaagaag cttctatcat
    26881 atctacaaat cccagtgatt ttatagcatt ttttaactga ccatatgtaa tattactatc
    26941 tatttgacct gttatagctg gagctactat tgcatacata ttttctttct ttgcaagttt
    27001 ttttgcaaca ggtgctatca aactcttatc agatattgct ccaaaaggac atgctgacat
    27061 acatgctcca caatttatac aattatcttc atgtatcatt gctttcatag tgtttctatt
    27121 aaaatccaaa gctcctgttg gacaaacgct tttacaaggt cttacaactt ctgatatagc
    27181 atcatattga catgaacttt tgcacattcc acaagcctta cataaatcat gatttatata
    27241 tgctctccca tttatataag aaattgcccc aaatttacat gcttccttac atctatgagc
    27301 caaacaccct ctacaaagct ctgtaactgt aaattcattt attggacatc tatcacatgc
    27361 tgcttcaact atatatataa tttgctcatc tgtgtccaaa ctaactgaat ctggatttat
    27421 atctccatat ttactatttg gagttaaact taatgcaagt tgcgcccttt caagaactac
    27481 ttctctctct ttataaacac aacatctata tctcgccttt tctcctttaa ttatttcata
    27541 tggaattttt tctatatttt cttttgttaa attttcttca atagatagtc ttgctactgc
    27601 tgttaaaact tcatgtttta acctttttag ttgcgtttca aattcaaaca taagtaatcc
    27661 tccctataaa tcacaccttt ttgctatatt tctactatta tgtatattgt attttttatt
    27721 tatttctata aattattata acataggcat atactttgtt aagttatata caaaatatta
    27781 tatatgtaat ttttatttta agtgtatata atttttattt tagtcgttca ataattaaat
    27841 atataaataa aaatagagtt gtccaaaagt taaacctcaa cttagaacaa ctctattaac
    27901 aatatgtatt tttgttgctt tgtattatct ttatttttct aaatttatat tactaaaatc
    27961 ttttatcttt gtatttgcta agacactttt cttagttgat tgagaatttt ttctatcaaa
    28021 agtggcttta acctttgcac ctgataagaa tttaccagaa ccatttatac aaccaccttc
    28081 acacatcatt ccttctataa aattaccttc tagctttcct aaattagcca tagtcatagt
    28141 tttcttgatt tctcttgaac cagaaatctt tacaggttca aaattaacac caataccttt
    28201 ttcagcaaga taattttcta tagcttttgt aagaccacca gcagctccaa aacctcttcc
    28261 aaaaattgat gcatcatcta tatcttcatt tgaacactta gccaagtcta tatcaaatgc
    28321 ctcaaataat gccattattt cttcaaaagt aagtacataa tcaacagctc cttttaattc
    28381 tttcttagct gcctcactct ttttggctgt acatggtcct atgaatacaa ctttagcatc
    28441 tggttcatga ctttttataa atctaccact agctatcata ggagacactg tagaagatat
    28501 ttttcctaca tgatttgggt atgttttttc tatataacca acaaatcctg gacaacaaga
    28561 atttgtcata aaagcttctc ccttttccat tctttcagca aattctaagc tttcatgaac
    28621 tgtaactgca tctgctccac aagctgcttc taatacatca acaaatccta gtgattttat
    28681 agcatttttt atttgaccat atgtaacatt tgcttcaact tgacctgtta tagctggagc
    28741 taccaccgca tacattttct ctttttgaac taactttcta gctacaggag ctatcaaact
    28801 cttatcagat attgctccaa atggacatgc tgacatacat gctccacagt ttaaacattt
    28861 atcctcatgt atcatagctt tcatattttc tctatcaaat cctaaggcat tagttggaca
    28921 aacactctta catggtctaa caacctctga gatggcatca tattgacaag cttttttaca
    28981 cattccacaa gccttacata attcatgatt tatataggcc attcctccga catgtgttat
    29041 agctccaaat ttacaagctt ctttacatct atgtgctaga catcctctac aaatatctgt
    29101 tactgtaaat tcatttattg gacaactgtc acatgcagct tccaaaatat atataatttg
    29161 ttcatcttct tctatatcca ttgtatctaa atctatctta tcttgacctg gttgtaaccc
    29221 catacttaca tttactctct cagatattat agctctttct ttataaacac agcatctgta
    29281 tgtaggtttc tcacctttta ttatttcata ctgaatatta tccattgctt ctctggtaag
    29341 gttatcttct tttccaagtc tagctattgc agttaaaaca tcatgtctta atttgtgtat
    29401 ttgtgtttca aattgaaaca ttttaatacc acccccgtaa atgattttat acgataccat
    29461 tataacacaa tatgtccttt tattaatata tacataaaat attatatgtt taaattttat
    29521 tttatcgaat atatatagtg tttttttgtt ttattatata atattattga tatttctgaa
    29581 atttcact
//
LOCUS       DAEORZ010000037        29545 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEORZ010000037 DAEORZ010000000
VERSION     DAEORZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.037"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            419..2431
                     /locus_tag="KRH39_001516"
     CDS             419..2431
                     /locus_tag="KRH39_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8511431.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNALIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNNKVKDILDINEDIYGKYIEDIFVDSLKVLLNEKDILDK
                     VVIFNRKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTTSPKMLECIDLAKKISKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYKWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSVNKNPSLKLKYNKNDILNILEILVLNKESDVGIGRGLILKTLLDKNLQ
                     ITEGKIKKIFEYLKKEELIICGSGRYGSKITQKGEDFYNKLKYKGL"
     gene            2800..4020
                     /locus_tag="KRH39_001517"
     CDS             2800..4020
                     /locus_tag="KRH39_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8511432.1"
                     /translation="MQQDLKLKYQTKELEKDFVIESCNSIKPWLINVRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKENADKTICIRADMDALPIEEEN
                     DIPYKSIYSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKLLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIETKYDTLNASVDTIKICVKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKISKIVEDTASAFGCVGTLHVSDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRANPSLGGEDFSFYTEHCKGAFFHLGCKNDEKGLIAPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            4057..5406
                     /locus_tag="KRH39_001518"
     CDS             4057..5406
                     /locus_tag="KRH39_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG8511433.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     IALITTIFICAIIYILIQVVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            5463..6083
                     /locus_tag="KRH39_001519"
                     /pseudo
     CDS             5463..6083
                     /locus_tag="KRH39_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(6191..8524)
                     /locus_tag="KRH39_001520"
     CDS             complement(6191..8524)
                     /locus_tag="KRH39_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG8511434.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSSLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEATHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(8521..9204)
                     /locus_tag="KRH39_001521"
     CDS             complement(8521..9204)
                     /locus_tag="KRH39_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8511435.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            complement(9275..10516)
                     /locus_tag="KRH39_001522"
     CDS             complement(9275..10516)
                     /locus_tag="KRH39_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8511436.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            complement(10527..11213)
                     /locus_tag="KRH39_001523"
     CDS             complement(10527..11213)
                     /locus_tag="KRH39_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8511437.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKILLTVR
                     RMGYKWNVIK"
     gene            complement(11451..11795)
                     /locus_tag="KRH39_001524"
     CDS             complement(11451..11795)
                     /locus_tag="KRH39_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511438.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKQLTVTGNEYTIINY
                     YMSNFSQYIIFAILLALMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
     gene            complement(11990..12253)
                     /locus_tag="KRH39_001525"
     CDS             complement(11990..12253)
                     /locus_tag="KRH39_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG8511439.1"
                     /translation="MEETRFCQCCGMPMGCTNELYGTNKDGSKSDDYCSYCFKDGEFT
                     ADISMEEMIEICIPPMVQSNKGMTEDEARSMMNNFFPTLKRWK"
     gene            12446..14074
                     /locus_tag="KRH39_001526"
     CDS             12446..14074
                     /locus_tag="KRH39_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HBG8511440.1"
                     /translation="MPKQNTIQIDYTIYGDNYQFKLPLNIDYIIPKDDSVRLLSQIID
                     EMNLEKLYKTYSRIRGNKVTPRQLLKIIIYANMNCIYSSREIEKACKRDINFMYLLGG
                     ASPPDHSTTARFRSIHFLPISKTLIAQFTNFLGDIKEISMDTLFIDGTKIESSANRYT
                     FVWKKAINKNIAKMMSNISDFILKCEIDFGIKVIYKDKIKIYHLKKLLKKLKKISKEL
                     NITFAHGKGKKKSLVQKSIEKLNNYVDRLKKYTKDLYIMGERNSYSKTDNDATFMRMK
                     EDHMKNGQLKPAYNIQFGVDSEYIVWVSSGHQPTDTTTLIPFLKAIEDYTKFKYPKIV
                     TDSGYESEENYTFLECNNQLSFIKPANYKISKTRKYKNNIGHIDNMDYDEKNDYYICK
                     NNKKLLANKIINRKSKTGYKSEITYYTCEECHDCQYKSNCIKRSNSKISLENRTKNLQ
                     VSKLFNEKRKENLNRIMSPEGCELRANRSIQAEGAFAQVKQNMKFRRFLSRGKENVLS
                     ECIIVAIAHNISKLHNKIVKNKRGIYLHHLKEVF"
     gene            complement(14587..17094)
                     /locus_tag="KRH39_001527"
     CDS             complement(14587..17094)
                     /locus_tag="KRH39_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8511441.1"
                     /translation="MKLNYVVKKYIMGNKKNTLLIIVSIVISTVLFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSGEYV
                     IGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNNE
                     KVDIKQNSFINKFMIKLFKDYGNIASKNLQRNKKRTNLSIASMVIIFFLMATIYTKST
                     SNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKW
                     FNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLIN
                     GKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCG
                     IVTTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITE
                     KTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREF
                     ALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSIIIKPFESINLINWKFI
                     GAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
     gene            complement(17103..17765)
                     /locus_tag="KRH39_001528"
     CDS             complement(17103..17765)
                     /locus_tag="KRH39_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8511442.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTSGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            complement(19234..19701)
                     /locus_tag="KRH39_001529"
     CDS             complement(19234..19701)
                     /locus_tag="KRH39_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG8511443.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDYFDTFVERLSTMRDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
     gene            complement(20158..21096)
                     /locus_tag="KRH39_001530"
     CDS             complement(20158..21096)
                     /locus_tag="KRH39_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG8511444.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILIAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIIV
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     LFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSNK"
     gene            complement(21412..22362)
                     /gene="hpdA"
                     /locus_tag="KRH39_001531"
     CDS             complement(21412..22362)
                     /gene="hpdA"
                     /locus_tag="KRH39_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBG8511445.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSVENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(22355..22612)
                     /gene="hpdC"
                     /locus_tag="KRH39_001532"
     CDS             complement(22355..22612)
                     /gene="hpdC"
                     /locus_tag="KRH39_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBG8511446.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(22616..25324)
                     /gene="hpdB"
                     /locus_tag="KRH39_001533"
     CDS             complement(22616..25324)
                     /gene="hpdB"
                     /locus_tag="KRH39_001533"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBG8511447.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            complement(25621..26637)
                     /gene="tsaD"
                     /locus_tag="KRH39_001534"
     CDS             complement(25621..26637)
                     /gene="tsaD"
                     /locus_tag="KRH39_001534"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBG8511448.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREILSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(26643..27119)
                     /gene="rimI"
                     /locus_tag="KRH39_001535"
     CDS             complement(26643..27119)
                     /gene="rimI"
                     /locus_tag="KRH39_001535"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBG8511449.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(27109..27825)
                     /gene="tsaB"
                     /locus_tag="KRH39_001536"
     CDS             complement(27109..27825)
                     /gene="tsaB"
                     /locus_tag="KRH39_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBG8511450.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIGLDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(27825..28277)
                     /gene="tsaE"
                     /locus_tag="KRH39_001537"
     CDS             complement(27825..28277)
                     /gene="tsaE"
                     /locus_tag="KRH39_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBG8511451.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(28416..29456)
                     /locus_tag="KRH39_001538"
     CDS             complement(28416..29456)
                     /locus_tag="KRH39_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG8511452.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
BASE COUNT        10311 a         4652 c         3308 g        11274 t
ORIGIN      
        1 taatttatgt tatttttaat aaaagataga attacaacta aattaaactt gacaaatcca
       61 ttctactcac gcagaataaa cttatacata tatattttca tatataattt attttaatat
      121 aattctatat ttataaaatt atattatcaa tagaatttat taaaataaat ttttttgggt
      181 gtatattata atttaatcga atagaaatga aaaatataca tacagtttaa gttttaagca
      241 acaattaggg ggatttggtg acaagatttt taattataag aaagaaagtg tcttaatgca
      301 acttttagaa agtcattata gacactttct tttttatttg ataaatttaa attatcaaaa
      361 ttagattgct ttatacaact attttgttgg aaactattta ttatataagg agaaataaat
      421 gaaaaaatca gtagctctgg taaatgatag tagaaaagat ttaatagact ttttagaaaa
      481 taatttgaaa ttagtttttg gagattctat aaatattaat agatacttca taaatgaaat
      541 aaatgataat gatattataa atgatgatgt gatattagta atgtcagtag aaagattaga
      601 caaaattata aataatatac tagataagaa aaaagtgatt gtggtaagaa gaacttttag
      661 ggaagataaa atttataatc tactatcttt accacagggt acaaatgctc taatcgtaaa
      721 tgattctgat gaaactactc tagagactat aagtttattt tataaaattg gagtgactaa
      781 tattagacca attccatata tgaatgataa taactataaa aatataaaaa tagctataac
      841 tccaggagtt ccagaaaaag tacctagctt tatatcagat atatttgatt taggtcacag
      901 atatatagat atctctacat ttatagaaat aataaattta ttacaaattg attcaaagga
      961 aatacaatct aatctagtta aatactcaga agaaataata agtttagata ctggtataaa
     1021 agataaatat aaggaattat ttttaaaaat agaagaatta gatacaatat taaatttatc
     1081 aaaagatggc atattattta cttcaaaaga tggtgaaata aacacataca ataataaggt
     1141 aaaagacatt ttagacataa atgaagatat atatggaaaa tatattgagg atatatttgt
     1201 agatagctta aaagttttat tgaatgaaaa agatatacta gacaaagttg tcatattcaa
     1261 taggaaatat ataaatgtaa ataaaaaaaa catatacaat agagatgaaa aaatgggaac
     1321 atattatagt ttacaagaaa taacatacat aaaaaaatta gaacaaaatc ttactaaaaa
     1381 gttaagagag aaaggacaaa tagcaaaata tacatttaaa gatataaaaa caactagccc
     1441 caaaatgctt gagtgtatag atttagctaa gaagatttct aaatctgact taagcatact
     1501 tatcagaggc gaaagtggaa ctggcaagga actcatagct cagtctatac ataacaactc
     1561 aaatagaaaa aatcaaccct ttatagcagt aaattgtgca gctgttcctg aaaatttatt
     1621 agaaagtcag ctattcggat atgacaaagg aacttttact ggaggtctaa aagatggaaa
     1681 acaaggatta tttgaacttg ctaataatgg aacaatattc ttagatgaaa taggcgatat
     1741 gcctttagaa ttacaaacaa aactacttag agttcttcaa gagaaacaga taatgcctgt
     1801 tggttctcac aatgtgatta atatagatgt tagaatcata tctgctacaa ataagaattt
     1861 agaacaaatg attgataata gccaatttag agaggattta tattatcgat taaatacaat
     1921 tccaataaat atacctcctc taagagaaag aaaagaagac atattaatta ttatggaaga
     1981 tttaattaat aagaaattag taatcacacc agaagcaaaa aaacttatac aaaactataa
     2041 gtggaaaggt aatataaggg aacttcaaaa tgttacatcc tatttaaaca ttatgtgtga
     2101 agatattgta ttagagaaag atttacctcc aaacttaagg tcttcagtta acaaaaatcc
     2161 atcattaaaa ttaaaatata ataagaatga tatattgaat atattagaga ttctagtttt
     2221 aaataaggaa tcagatgttg gtattggtag aggattaatt ttaaaaacac tactcgataa
     2281 gaaccttcaa attacagaag gaaaaattaa aaaaatattt gaatatctaa aaaaagaaga
     2341 acttataatt tgtggttcag gaagatatgg aagtaaaata actcaaaaag gagaagattt
     2401 ttacaacaaa ttaaaatata aaggtctata aatttataat ctattaaaca aagaaatcta
     2461 ttcaggagct aaatactcct tcaataggtt tttttatttt tcaagtaccc caaaattata
     2521 acttattttg tacataaata ataggaatta ataggaatag gatatttttt tatttcctat
     2581 ttcttttata taagctaagc atataagata aattttctcc aaaataatat tttttactga
     2641 aaattgcaac tataaaatcg ttttttacat tatatatatg gataaatgat tctgtttatt
     2701 caatattctt ataaaaagta aaaatcttca tctaaaatta tggcataata attgcttatt
     2761 atattaacca aacaataaaa agcaattgga ggaataaaaa tgcaacagga tttgaaatta
     2821 aaatatcaaa ccaaagaatt agaaaaagat tttgtaatag aaagctgtaa ttctataaaa
     2881 ccttggctta taaatgtaag aagggaatta cataaaattc cagaattagc ccttgaagaa
     2941 aatcttacta aacagaaggt catttcttat ttaaaagaaa taggaattga ttatatggaa
     3001 tttacaaaac acaatggaat tatggcatat attttaaaag aaaatgcaga taaaactatt
     3061 tgtataagag cagatatgga tgcccttcct attgaagaag aaaacgatat accttacaaa
     3121 tcaatatatt ctggaaaaat gcacgcttgt ggccatgatg cacatacaac catgttactt
     3181 ggagcatgta aagttttaca ttcaataaaa gataaactta atgtaaatgt aaagttatta
     3241 tttcaaccag cggaagaagg ttttggagga gcaaaattct tagttgaaga tggatgttta
     3301 gaaaatccta aggtcgatta tatattcgga cttcatgtta tgccacatat cgaaacagga
     3361 tttatcgaaa caaaatatga tacattaaat gctagtgtcg atactataaa aatttgtgtc
     3421 aaaggtaaaa gagctcatgg tgcatatcct gaaaatggta ttgatgcaat agttacagct
     3481 tcacaaattg ttacatcatt acaaactata ataagtagaa acttagaacc aaataatgct
     3541 gctgttttga ctatagggaa aatatatggt ggtgatgcac acaatgtaat atgtgaagat
     3601 gtaaaattag aaggcacctt aagaacctta aatagcaaaa caagaaactt tatgattgat
     3661 aagatatcta aaattgttga ggatactgca tctgcatttg gctgtgttgg aactttgcat
     3721 gtaagtgatg aaaattatcc tgctgttata aatgaaaaag aattagtaga cacagttata
     3781 tcaagtacta aagaattact tggagaggaa aaatttatat tgagagcaaa cccttctttg
     3841 ggtggtgaag atttttcttt ttacacagaa cattgcaaag gggcgttctt ccatttagga
     3901 tgtaaaaatg atgaaaaggg attaattgca cctcttcata cctctagctt taatatagat
     3961 gaagattgtt taccaatagg tgttatgatg catgtaatga atactttgta ttttaattag
     4021 tacagtaatt aataaaagaa taggagataa attaatatgg aaaaagctaa gaaattaggt
     4081 ctatttagta tgatattatt agggattaac agcataatag gttcaggtat attcttacta
     4141 ccaggtaaag tttataatct agcaggtcaa aacagtatgt ttatatacat ttttgctaca
     4201 ctcttagttc tatcaatact tttatgtttt gcagaagttg gaagcatgtt tgataagaat
     4261 ggaggagcat atctatactc caaaaaagct tttggagatt ttataggatt tgaagttggt
     4321 acaatgtctt gggttattag aattatatca tggtctactc tagcagtagg atttgcaaca
     4381 gctttaggtt cattttggcc agaatctgcc actgaataca aaggttatat agctgcaata
     4441 ctcgtaacac tactttcaat aaatagctta tttggaatta aaagcacaaa gataatgaat
     4501 aatgtaataa ctatagctaa attagtacca ttaatagttt ttataatagt tggaatattc
     4561 tttataaagt ttgtaaatat tgttccttca ggaaatgtag ttaactctag tatgggtcca
     4621 gccattatat tagtatttta cgcatttacc ggctttgaat catttattgt ggcaagtgga
     4681 gaaatggaaa atcctaaaaa aaatcttcca attgcattaa taactacaat atttatatgt
     4741 gcaataattt atatattaat tcaagttgta tgtatgggta tacttggaga tagactattt
     4801 gaaaacagta ttccaattgc agatgcttca agtgtatttt taggcaatta tggtaaggtt
     4861 tttatatctg tagcaacatt aatatctata tttggtataa atataggttc atcaatagta
     4921 actcctaaat gtggttcttc tctagcagaa gaaggttctc ttcctgcatt tataggaaaa
     4981 acaaataaat atggtgctcc atatgtagca ataataatat ctttaatttg ttgtatacca
     5041 cttgtgctaa ctggaagttt tgaacaactt gcagtaatga gcgtaattgc aagatttgct
     5101 cagtacatac caacttgttt atcagtaata gttttaagaa aaagaacaga tgttaaggcc
     5161 tcttttaaga ttccatttgg tcctgttata ccaatggtag ctattttagg tagcttatgg
     5221 ttgttacaac aagcttgggc agaagatatt tcaaaaccta taactcaaaa tagagtttta
     5281 ataggattag gggcaatgtt gttaatagct cctctatata tatttatgaa aaaaaataaa
     5341 gaaatcgagc ataaaaataa atcagaggat tctaaattat atatttcgag agaaaaaatt
     5401 caataatatt ttacaaacta tttaaaaatt atttttctaa tactatagaa ataattagaa
     5461 atggagatgt tgcatatcta aaagtagttg ataacacgga tattggtact tttatagata
     5521 tgggacttac aaaagacata ctagttcctt ttaaagctaa gacttatcca ttgtttagag
     5581 atgaaaaata tttattctat atatacttgg ataaaagcgg aagaatagct gctactacag
     5641 acatagattc atatttagaa acagaccata cttacaatgt aggagatgtt gtaaatggag
     5701 ttgtatatgg attccaaacc aacaactctg ctatgatatg tgttgacaat aaatatgctg
     5761 gtgtaatcct tcataatgaa tactttacag aattaaaagc tggggatgtt cttgaaaatt
     5821 tacatgttat aaaaatatac gatgatggta aattaggact ttctcctaga ggaaacagaa
     5881 aagatgaact tgatacctta gaaaataaga ttttaagcta tttagaaggt tcagatggat
     5941 atatgagatt caatgataaa tctgatccta aagatattta tatcttattt aattcaagta
     6001 agaaaaattt taaacgtgct cttggagtac ttatgaagaa agacttaatt tatcaagatg
     6061 aagatggaac ttatctaaag taaattatat aacacaaaaa tcgaagtaaa ttatgaaaga
     6121 tattttctat ttcatattta cttcgatttt ttatttaaat tactacctct atttaaatta
     6181 ctacttatat ttattgtgca tttactacct ctattatgtc taattcacaa atctttttaa
     6241 ttggttttct tactgcaagc aaagttatta ttatcatagt tactacaatg ataaacagta
     6301 aatcaaaagg tatttgccag tctatatgga attttaatgt tactaaggat acaaatatat
     6361 atctatgtaa tattaatcca agtacactcc ccaataaaca gccacttaca gcatatgtac
     6421 ttgattctac tattaccatt tttcttaatt gcttattgga catgcctatt gcacgcataa
     6481 taccatagta atttattcta cttgtaacgc ttatattcat actgtttatg atattaaaca
     6541 tacttataat cgcaattata aatacaaacc catatacaaa aactgccata gtgtaaaatg
     6601 aatttttagc ctctaagtta ccttgtcgct tatctcgaac ctctaaatta tcatctagaa
     6661 catttcttat attttggatt aaatcttcag gagcatcttc atcaacttga gtatcaatta
     6721 ttatatagtc ttttatatct gttatactag taaaagtatt ttcagatgtt ataatatttc
     6781 caatatattt gtctttaaca tcaaatgaaa ttgaagaaag tatacctgct acctctatct
     6841 ctttcttaga atttccattt ttaatatata tcttatctcc aatattaaac ccatgacctt
     6901 cttctaccaa tccgatattt tctgactcta atttatcttc attgatttcc ccttttacta
     6961 gatacttttt ggcccagtca aattgatttt tttcataaga aattagctga gatgttgcct
     7021 ccttattatt gtatgtagcc tctacatccg tcatcattct actatatact ttttttattc
     7081 ctttgatatt acttatttca ttaatatcgt ctttagtaag aacagactta aagtcagcgt
     7141 caacaataga tatatgagta gcttctggtt ttataggttt aagtgcctga tacataaagt
     7201 ctataaatac actaaaacct aaaaacaata caatactaat agcaaatgaa gaagtcataa
     7261 gtataaaaga tttttttctt gaaaaggcat gatttattcc catagaaaca tcaattttag
     7321 aacttcctgc gttagcaatt ttacttgctt ttccactagt taaaatttct ttgaacatta
     7381 gattaccacg tatagcactt agtggagaca ccccagaagc tttttttgct ggaacaatag
     7441 ctgataatat tactgttaaa aatcctacta ttattccaaa cacaatacca ataaagctaa
     7501 cctttaaaat tggcatcgaa ccaaataact ctgtattcac atatttgaga aatatagagg
     7561 atataatggt tacaatacat cctaataaca atcctagtgg aatagccttt acacttagaa
     7621 ttaaactttc taaaacaaca aattttttga cttggagttt tgatgctccc aagcaacgta
     7681 atagtccaaa aaactttatt ctctctatta cacccatatt aaagctatta tatatcatga
     7741 taatacttgc tattaacaca agtataaaaa gtcctcctgc tgttaagtaa agtggtttag
     7801 aataatcaga gtcaccttga cctataatag aaagtaattt ttcattttta ccgatttgtt
     7861 tatcacttaa attgaaggta ttcttaatat cagaagtagc ttttctaata ttaaccttgt
     7921 ctttaaatgt aatataataa taatcatatg tttctgattt tttgatagct tgtgtaccta
     7981 atattgaaag cattaatcca tgcttatctg aggctcttaa gcttgataat gatgaatatt
     8041 cacctactat cttataattt ttatttctac catctgctaa ttttattttg ataaaatctc
     8101 ctacttttag attaaagctt ttaactgcat ctacatctat taaagtctca ttctccacaa
     8161 caggataacg accttttatt atttctagac ctaattgttt tgctaaatct tcatctaaac
     8221 cttgtatcaa aatctccttt gatttaaact ctccattatc aatgacttgt cctctaccat
     8281 agtgagctat atctactcta ctacttattt ctgatatagc ttcttcatta agatttttaa
     8341 gtactatgtg ccaatttcca tcatctttta taaattgtac tttttgacat tgtatagcca
     8401 tgtctgctag tccaaaaata gcagtaacta aacatactgc aatgacaata cataaaattg
     8461 tagttctatt ttttttctta tgtacctttg catattgtgg tactagttct aaatagcttc
     8521 tcattctcta ttcaccccta ggtctttaag cattccatca gaaacctgta gtatcctatc
     8581 tgctgtagaa gctatatttg tattatgagt tatcataata atagtttggt tatatttttc
     8641 tgctgtcact tttaaaagtg aaattacatc actgctattt ttactgtcaa gatttcctgt
     8701 tggctcatct gccaatataa ttgatggctt tgtaatcaat gctcttccaa ttgcaaccct
     8761 ttgttgctga ccaccagata attgatttgg aagatgactt tttctatctt taagacctaa
     8821 aattgtcaat acttcatcta gataaatagt gtctggtttt ttgtaatcta gtagtaaagg
     8881 aaatattata ttttgttcca cagttaattc gggtataaga ttatatgatt gaaagataaa
     8941 acctatatta cgacgtcgaa aaatagtctg tttttcttct ttcattgaac atatttcttt
     9001 accatcaaca aatatttttc ctgaggttgg agtatcaaga cctcctaaaa gatttaataa
     9061 tgtactctta cctgagccag attctccaac tacagtaaca aactctccct ttgaaactgt
     9121 aaatgataca tcttttaatg cctctacttt tgtatatcca tttccatatg ttttacataa
     9181 attattaacc acaagaatat ccatttgctc tatctccttt ctgatgatta atataagaat
     9241 aattcataca ccttactgta agatgtatcc tatcttacat attagtaagc tttaaaaaac
     9301 ttagagtaaa tgtacttcct tgtaaaaaat tactttctac agttatggtt ccattgtgct
     9361 gttctatgat agatttaact agtggcaacc ctaatccaag gccttgatta ctttttgaaa
     9421 acttactcct ataaaatctt ttaaatatat gatgtatatc ttcaatatga attccatttc
     9481 cggtatcact cactgaaata atagttgtta taggtgtttc aacccattct atcattattt
     9541 tatcgttctc ttttgtatta tctaatgcat tttttataag gttgcttaaa gcttctgata
     9601 tccattcata atcgcaaaac aagtttgtat tttctagacc tcttatagag atactctttc
     9661 cttctttttc tgctctagtt tcaaaactat ataaaatttt agtaatcaac ttttttacgt
     9721 taacactagc tttgttaaga attattgtct ttgaatctat ctttgtaatc tttaacaagt
     9781 tttgtatcaa tgattctata cgctctaatt cattttgact cttctgcaca aaattctcaa
     9841 caatttcact attatgactc tcttcctgca atatttgatt atacatcatc aatgctgtaa
     9901 gtggagtttt tagttgatgt gatatatctg aaattgtatt tttaagaaat tctttattct
     9961 gcttttcttt caatatatgt gtgtttagag atgttgacat agcatctatt gaaccaaata
    10021 actttgataa actaccttct tcataagtat ttaaccgtac atttatattt ccattcataa
    10081 atgaatcaat tttataacta gcttcttcta tcttgttctg ttgcctaaaa aaatacacat
    10141 acaatataaa aataatcata gtaaagaaca caaataaata caaaaacata gtttttatat
    10201 atttaccttg aaacaatttc acacttggca taaaactatc atctgtacca aagtcatatc
    10261 tagctgtatt taagatttct tttcctttta aagtatattt atcttcatta gtagtaaata
    10321 ctgctggtat ttttgattta tcaacaccca tactatttaa gtatccagct atattataat
    10381 catgttctaa cagtgcactt ttataatcat tagttatatt tataactact atttgaccta
    10441 ctactataaa catcaataaa acggatataa tcaccaaaaa aaatattttt atgtctttat
    10501 tagaaaatac cttcatatta acaccctcac tttataacat tccatttgta acccattctt
    10561 ctaactgtta atagaatttt tggattttca ggattatctt caatcttatt tcttaacctt
    10621 cttacatata ctgacagagt attatcatct acaaaactac catttccatc ccataactta
    10681 tccaatatat tttttcttgt aagtacaata tttttatttt tcataagtaa gcataataat
    10741 ttatattctg ctgtagtaag ctcaagctca atttcatttt taaaaacacg attttcaagt
    10801 aatttaacag taatattatt tgattttagt tcagttacat tttcacttgt aaaattataa
    10861 cgtctaagta aagcttttat tcttgaaatt aactcattta atttaaaagg ttttgttatg
    10921 taatcatctc cacccatatc aagtcccatc actacattta cttcttcatc agaagcagtt
    10981 aaaaatatga ttggtacatt agaagcttct cttacccttt tacaaatatc aaaaccatca
    11041 ccatcaggta aggatacatc caaaagcagt aaatcataat ttttttcttt ataaatttgc
    11101 aatgcttctt ctactgttct tgctacatca acactaaatc cattcttttg tatagaatat
    11161 tctaatccat atatcaaact taaatcatct tcaaccaaca atatactact catctcaatc
    11221 acttccattt ttttattttt tactttatat atattatata cctaacttat tatattttta
    11281 ctcttttaaa aatacaatac cttgaaatca atgatttaaa tattttaaaa ttatttttat
    11341 atgattttat atagttagtt taatactttg aaatcttaat aataaaaaaa gcttaaaata
    11401 cactaagtat tctaagcttt attcaatcac attttatata tttatattat ttattttttt
    11461 aaaatactat tttcaatatt gcctctatta aacatttcat tatcattatc taaagagata
    11521 tcacttatat ttctaacagt tgttgaatag ttaccactaa gttctatctc actgaaatgc
    11581 atatgcacta tcttttgtat tacaaatccc attaatgcaa gtaaaatagc aaatattata
    11641 tactgactaa agttactcat ataataattt ataatggtat attcatttcc tgtgactgtt
    11701 aactgttttg tagatagcat agtatatata tattcagtac aatttataaa tacatatata
    11761 ccataaatag ccaacactac agataaacaa tacattgtga ttgaaatctt tgatattttt
    11821 ttaaattgct tcactccaaa acctttttta aacttctcaa taaaatcttt cattttaact
    11881 cctccaatac attatttaat aaaatataat aatattagta tatagagtta atgtgtttat
    11941 agtattaatt tttcatgaat tttatatgag ttattaattt tatataagct tatttccagc
    12001 gttttaatgt tggaaagaaa ttattcatca tacttcttgc ttcatcttca gtcatacctt
    12061 tattactttg aaccataggg ggtatacaaa tttctatcat ctcttccata cttatatcag
    12121 cagtaaattc accatcttta aaacaatagc tacagtaatc atcgctttta cttccatctt
    12181 tatttgttcc atacaattca ttagtacatc ccattggcat accacaacat tgacaaaatc
    12241 tagtttcttc catatataat cactccttgt atatttttct ttggctccta accatattta
    12301 aattatagca agagtaatat gacatgatta tgtcatatta aaaataaaaa ggggctgtcg
    12361 cactagaaaa tagtgctacg cccttttttg tataaaaaat aaatcccaag ttcaaaagag
    12421 cctgggattc ttgtataata attgtatgcc aaaacaaaat actatacaaa ttgattatac
    12481 tatttatggt gataattatc aatttaagtt accactaaat attgattaca taattccaaa
    12541 agatgattct gtaaggttat taagtcaaat tattgatgag atgaatttag aaaaattata
    12601 taagacttat tctcgaatta gaggaaataa agtaacccct agacaacttt tgaaaattat
    12661 aatatatgca aatatgaact gtatttattc ttcaagagaa atagagaaag catgcaaaag
    12721 agatattaac tttatgtatc ttctaggcgg agcctcacct ccagaccatt caacaactgc
    12781 aagatttcgt agtattcatt ttttaccaat aagcaaaact ttgattgcac agtttacaaa
    12841 ctttcttgga gatatcaagg agatttcaat ggatactcta tttattgatg gcacaaaaat
    12901 agagtcatct gcaaatagat atacttttgt ttggaaaaaa gctataaata aaaatatagc
    12961 taaaatgatg agtaatatat ctgatttcat attaaaatgt gaaattgatt ttggtattaa
    13021 agtaatttat aaagataaaa tcaaaatcta ccatttgaag aaacttttaa agaaactaaa
    13081 aaaaatatca aaagaattaa acataacatt tgctcatgga aaaggaaaga aaaaatctct
    13141 ggttcaaaaa tctatagaga aattaaataa ttatgttgat agattgaaaa agtatactaa
    13201 agacctttat ataatgggtg aacgaaatag ttattctaaa acagataatg acgcaacttt
    13261 tatgagaatg aaagaagacc atatgaaaaa tggacaactg aaaccagcat ataatataca
    13321 atttggtgta gattcagaat atatagtttg ggtaagttca ggacatcaac ctactgatac
    13381 aacaacatta ataccatttt taaaagctat tgaggactat acaaaattta aatatcctaa
    13441 aatagttact gattctggat atgaaagcga ggaaaactat acatttttag agtgtaataa
    13501 tcaattatct ttcataaaac ctgcaaatta taaaattagt aaaactagaa aatataaaaa
    13561 taatattgga catattgata acatggatta tgatgaaaaa aatgattatt atatttgtaa
    13621 aaacaacaaa aaactacttg ctaataaaat aataaatagg aagtcaaaaa caggttataa
    13681 aagcgaaata acttattaca cttgtgaaga atgtcatgat tgtcaatata aaagtaattg
    13741 cattaagaga agtaatagta agatttctct tgaaaatagg actaaaaact tacaagtatc
    13801 taagttgttt aatgaaaaaa gaaaagaaaa cttaaataga attatgagcc cagaggggtg
    13861 tgaacttaga gcgaatagaa gtatacaagc agaaggagct ttcgctcaag taaaacaaaa
    13921 tatgaaattt agaagatttt taagccgagg taaagaaaat gttctatcag agtgtattat
    13981 cgtagcgata gctcataaca tttctaaact acacaataaa atagtaaaaa ataaacgtgg
    14041 aatatatctc catcatttaa aagaagtatt ctagatttat tatttataat caaatattat
    14101 ctaaaaaaat ggtattttta tacctttctt tgtcatgcat aattttactt agaacataaa
    14161 ctttatataa agcaattaaa aatatgccta ttaagatact taaaaaggag ctctcactga
    14221 acaaatttaa tttgctcatg cgacagctcc atttttttca agtaactgtt aaatatttta
    14281 atataactta ctatctttac aacacttaat atttgttatt tatcataaac aaggttattg
    14341 tattatcaat aatttttagg ttacacttat tattagtctt gtgttaaaac gacagtagac
    14401 ttaagagtag agttttatta agccaatatt tatatttgct atacattgta cccctattac
    14461 accatacata taacttggat aacttatttt atttttattt ctaaattatt taaaatcaat
    14521 atctacacca ttatcgataa ttgcaattaa attcatataa aacctcttta atttataggc
    14581 tgttcactat aaagtcttta tttcttccat aaaattttcg ttttgaattt tattcaatgt
    14641 ttttagtgta gttatcatga ttatagatac taaaataaat actattagga taatagcccc
    14701 tataaatttc caatttatta aattgatact ttcaaaaggt tttattatta tacttcttac
    14761 aattaaaata ggtattatag atatacaaaa gcttactata atatatatat aactttccaa
    14821 tttaacaatt ttatttattt catgttttga tattcctatt cctctaaata gtgcaaattc
    14881 cctacttctg ctattaatac tagtaactat agtattgcaa cagttaaata aagataatat
    14941 aactataatt ccagttacta ataccataat tagtgttttt tgattatcaa ttttttcata
    15001 ttcagctgtt tcagctttgt atggaattaa aattccttta tttgtttttt ctgttatttt
    15061 gtttaactca ctttcaacat atttatcatt agctagttta cttgtccata tgtcaaatct
    15121 ttcataagat gatattcctg taaatttatt gaattgattt gttgatatta ctccaccaaa
    15181 ttcagctccc acttgtgagg ttactggtaa tgtagttaca attccacata ctataaattc
    15241 ttgagatatt gttttggtaa aattgtttgt ttttgaatct ataatatcaa agtatacttt
    15301 tattctatct ccttttttta tattaagtga atcactggat ttctttgtta tatacagata
    15361 tggttgttta ctaaatttat ctatattttc ctttccattt atcaagaaat cctctagtat
    15421 atctgtatct tctattccta aaaattcaaa agaattatta tataccttaa ttccatcttt
    15481 tacttctgat ctagcttcaa tattatcttt attttttttc caataatcag agttcttatt
    15541 tatactttta tcatcaattt ttatattaaa ccacttatgc ctatatgagt ttacttttgt
    15601 tacaccatca attgccttta tctcttgtag tacattttta tcataacctt tattatttga
    15661 acgtatagta cttatatcaa tattatgtaa taaataatct ccttgtatcc aatattttaa
    15721 tacaccatca cttaaaaagt tactagtaga ctttgtgtat attgttgcca ttaagaaaaa
    15781 tataattacc attgatgcta tagataaatt tgtacgtttc ttatttcttt gtagattttt
    15841 tgatgctata ttaccatagt ctttgaaaag ttttatcata aatttattta taaaggaatt
    15901 ttgttttata tccacttttt cattatttct tcttatacaa atcattggat cagttttaac
    15961 tgataacata agaggttttg aaactgatat tattatagtt cctaaactta ataatataga
    16021 aattatgtat gtgtttatat agttctggtt ttttatagaa tttatatagt tgacattgta
    16081 tattactcct ataacatact cccctgataa actagctatt atcccaccta aaattagacc
    16141 tatagaaata cttataatag agtatataga tacttctaaa atcattattt taaaaatatc
    16201 atttggatta aaccctaaag ccctaaatat cccaaaatcc tttattctat ttgatactaa
    16261 tatataaaaa atattataaa ttaataatat agttgctagt gctagtacta tattaactat
    16321 tatataaggt atttttaaat tcatactatc agatatagct attaataatt cattattaaa
    16381 ataaatgtta ttctcattca aatttccatt tttaataagt ttttgtgatt gtctctgtat
    16441 atttttttcc tttttaaaag taaacataac ttgatcatat ttatttttag taggaatatt
    16501 cttgatagca caatcgttac ttataaggcc aattgatgtt ccttgagctt ttaaaatagg
    16561 attactattt aagataccag ttattttaaa ttctttttcc ccagtatata aatcttcttg
    16621 atctatccct tgtcttctat aatctaatgt tatccttttt cctatagggt tttttatctt
    16681 tttttgttta atataccatg aatctatagc gatttcattt tcagctactg gtaatttacc
    16741 tttttctagt gtgtaaatat catttagttc ttcagcaact ttatcctgag atagaatttg
    16801 tagtgtttga cctttaccaa tatcactaat tcctaaaagc attgattttc caactttatc
    16861 tatacttgtg ttattttcta tatattttat ttcttcgtct tttggattga tatattgtgc
    16921 atgttttaat gtaaattcat ttttagcttg atttatcatt aagtttgtcg catcttcact
    16981 tattatattc ataattaaaa acaacactgt tgaaattaca atacttacaa ttattaataa
    17041 tgtatttttc ttatttccca ttatatattt ttttacaaca taattaagtt tcataaatct
    17101 acctactctc ttataaatcc atcttctatt tctataactc tatcagcata ttttgttata
    17161 tctatattat gtgttatcat tattattgtc tgattatatt ttttttgaga ttcttttaat
    17221 aaacttaata catctgactc tgtctttgta tctaaatttc cagtaggttc atcagcaaat
    17281 acaattgatg gtttatttac aagagccctt gctattgcta ctctttgttg ttgcccacct
    17341 gataattgat ttggcaaata cattcttcta tcttttattc ctagtaactc catcaattca
    17401 ttaatataag cttcatcttt attttttata tttatagttg gtagttttat attttcttca
    17461 acagttaaaa ctggaatcaa attatatgat tgaaatatta atcccaactt ttctttacgt
    17521 aatgcagatc ttttcttttc atcaagagat gatatattta tattatcaat aatcacatca
    17581 ccacttgttg gagtttcaag tcctgccatt atgtgtagta aagtactttt cccacttcca
    17641 cttttaccta ctatcacaac aaattctcca ctatttattt ttagatttat ttgacttaac
    17701 gctttcacct ctgtttccga tttaccatat attttattta ggttttttat ctctaaaata
    17761 ctcataattt actccttgtc ttacatgtta tttacactta tcaattttat agtttaacac
    17821 ttgtatatga atctataagc ttatctttat aacatctttt ttgttgtgtg tcttttattt
    17881 cctattcaaa tccaaaatca gctaaacagt ttttattttg tccatttacg tctaatattt
    17941 ctacttttta ctatatatac aatctaatat gttacctata ttataatttt ttagacctgc
    18001 agcgaatgta cacaaataaa tcttctatcg tatttagtgt aacattcact ctacacctta
    18061 aatattcaaa tattgagttc attttacgtg cagttttaat agctttatta aataatccta
    18121 tattattttt ataaattcga tttttactat ggattttgca atctctatct atacttattg
    18181 atatgtagcc acagaaacta tttttataat tttcaaaaaa ttcttctgtc ccttacttcc
    18241 atataattcc attaattatc atgccatatc ttataattga attttatttc ttatatctct
    18301 tttaccaacc ttcttattta ggaaaagttc ttcaccatga aattgtacag tatataccta
    18361 tttctttgat tctataatta atctttttat ttagtaacct attttctaca tcataactta
    18421 tatttcctgt ttcctcagtt tcatatttat aacaatagtt acaatctaaa ttgctaattt
    18481 tagttaccaa tactgtgctt tacatgcttt gcctctaaac tacttatgtt aatattttct
    18541 taattttttc acacctatct tcaaatataa aatctttatc taaaagatta ttgtattcag
    18601 ctagacatga ccaacaaaat gatttaacta aatattcttt acattcatta ttacataact
    18661 ttaagttgaa aaactcatca tatccaaaat tcgttgatac ccatattatt aatataaatt
    18721 acaatgtctt tttgaatatc taaaaaaata cttttataac ttattttata tatcatacac
    18781 tatattattg ctttcataat ctataaaggt ttcatatgat acaaacttct aagatgggat
    18841 ttttggtctt taacaacgat tataataata tttatttata tttctgattt aaacattatt
    18901 ttacttgaaa tgtttgttaa ataaatgaat taaaactaaa ctttcattat atttttaatt
    18961 tattcaatta aattttagca tagttatata aaatagtata tataattata caagataaga
    19021 gtaaatataa ctatctttta tatattaata cttttttcta aatctaaact ttagtataaa
    19081 tatataagaa atatcataat atttttaaaa actcaaataa agctaaacaa atattatatt
    19141 ctttatttta gaaaaataaa aactgtaagc aaatttgttt tcacacaagt ttacttacag
    19201 ccttcacctc aactttattt taatataatc catctattct aaaactgatt caatatattc
    19261 gacagttaca tccattcttt tagctacctc atctattgaa agaccactat ctaaaaaccg
    19321 tctacacaca gatgacatag tttctccaac ctcaataata tgcccatcta aatcataaaa
    19381 tctaattgct ctttgacccc acctatgctc tatagctaga tgaacataat ctatatctct
    19441 cattgtactc aatctttcaa caaaagtatc aaaataatct tcctcaaaat acagctcagc
    19501 atcatttcca ttgaacttaa cttcactgtc actcttatta ataaactcta accaaagctt
    19561 tttttcttgt aatgatatat tcccttctaa tacaacattt tctccaaaat cacagattac
    19621 ctttaacccc aaaacatcat aataaaactt ttttgatttc tcaatatctt caactactat
    19681 taaaacattt aaaaacttca tattacctcc ttaatataca catactttat ttataatata
    19741 taagtagaat taaactacac ttatttcaat aagatatgat atgaataaaa aaccataaat
    19801 cttattgata tagttaaatc cattttatta tataatatac cttttaatct tgtaaaaaac
    19861 agacatttat catagttgtt aataaaatag aactctctaa gaatcatttg tataatacat
    19921 ggtaaatctt gtttgatgga gtaaatacag aaatgctaaa tctaatagca taggaattta
    19981 tcatgatata ggtaagatat aagaaattag caatagaaat ataagtatta gttaaaagtg
    20041 aaaatgtcaa aatagcccat aaaataaggc tattttgaca tataaatacg cttaaaagaa
    20101 aatcaaaaac ttgctcaata tttataccaa tcaactgatt tttgatatta ttcacactta
    20161 cttattacta acaaaatcat acttagaatc tctatagtaa taccatacaa atataaagct
    20221 aagtagatta acaaaagtag tcaaaattcc accttcaata ccaaacagtc ctccatttaa
    20281 tagattacca tgagtgattt gtgtagttat aatccctgga acttgaagtc cacttacagg
    20341 cataccatat ataatcccct gaaatacatt ccatgcaaaa tgataaccta tacacatcca
    20401 tatattcgac gattttataa acatatatga aaaaagaatc ccaacaatta taatattaat
    20461 tacagataaa aaagtaacat caggattcca tatatgaatc aacccaaaaa taacagatgg
    20521 aacaatcatt ataaaataaa tatttttaca tcttcgtaaa actgccataa aaaaaccacg
    20581 atttaaaatt tcttctgcaa aagcaattaa aataaagaca agtatccaag aaactgttaa
    20641 aggtgaaatt ttgggtaccc atgaagtaac atgaccacca ccaattgtta ttgttataat
    20701 aaagattact gtacagttaa atattcctaa caacattcct acatatcctt cttttttctt
    20761 aattctataa gaatgtaacc ccatttcact aataggattt ttcataataa atctccatac
    20821 aattatagga ataataatca taagtgaatc tgttagaatt tgaaatacaa tctgtaaccc
    20881 aacatcattt aaccaattag catattctga aaaatagttt gtttttaaat taatgtcacc
    20941 tgtagcaacc aggatttttc ttattatttc cacaagtatc atgcttaaat aatactgaag
    21001 tatgtaaaag gtagccataa cagctataat aatccaccct gaacgtactt ttttgtcttt
    21061 attcttaata atcaagttgc tccatgattt gttcatatag atgtcttcct cctaactatt
    21121 atttttagtt gtttctaaaa tatattctat taaatttttt catagtagta ttttaccaca
    21181 aaacttctgc ttcaatgaag taagcgtaat atattctcta actgtaattt aaactataaa
    21241 aaatatataa agtatattta aagcaatatt tatggacata aagcaatata aacaatatat
    21301 aaaacatata aaactttact taattatttc tacataaaaa ccacataaaa aaggatgtaa
    21361 ctctagcttt tagtgtaaca tccttttttt aattaaaact tgtatttaat tttagaaagc
    21421 tgtctcatga ccaacataac aagctatacc attatccaaa aatatatctt gtaattcttc
    21481 aagatgacct tcatcaacat cacccttatc agaatactca tattccttgc ctaattgaat
    21541 ccattttgat tctcctaatc tgtgaaatgg taatagatta atttctacca aattattttt
    21601 gtgcataaaa gatataatat cacttatatt ttcaacacta tcattaaacc cagagataac
    21661 tggtactcta agtactaatc ttccattcca atcagaattt gcaaggtttg atatattttc
    21721 taatattaaa tcattataca cacctgtttg ttctttgtgt ttttctctat ccatatgctt
    21781 tatatcaata aatgcaaaat caatatcgtt aaatatttta ttaaaaactt catttgatac
    21841 acatgcactc gtttcaatgg cagtgtgtat atttacctca tgacacttta ataatacttc
    21901 atgtaaaaat tcatgctgaa gtagtggctc acctccacta aatgttactc ctccattact
    21961 tctccaattg ttagaatcac gtttaattac ctgcacaagt tcatctacag tgtaaggttt
    22021 tgcacataat ttaaacgcat tataataaca tgagttgacg cattcaaaac tttcacagtc
    22081 cttacagatg ttccaatcta taactggctt attatcaaga ttaaagctca atgcaccatt
    22141 tttacactta ccatgacata cagtacatcc attttcatat tgacaagata attcactaaa
    22201 catcatatgt ggtcttacag tccaactttc tggatttgca caccatttac aactcagtgg
    22261 acatccattt aaaaatacag ttgttctaca acctggacca tcatgaactg aaaagctttg
    22321 tacatcaaaa atcatgcctt ctaattgctt ttgactactc attaaacaca tgtccttcac
    22381 aagtaattgc attcaatgtt gaatataccc agtcttcttt ctctaggcca acacatttgc
    22441 ctataccttc atcattagct tcaacaaaat ttttacaatt tttacatttt ggcattactt
    22501 ttatctctgg acatgctgca tcatctaaat taatcatttg ttttgataat ctacaaattc
    22561 ctttggttgc atctactgca caaaaattca tacaatcact atgctttctc atattttaca
    22621 ccccttcata ctctgttcta gcaattactt catcttgtat tggtttacct aattcacacc
    22681 aatattgagt aaacccagca acacgtacca ttaattgacg ataattgtca ggattttttt
    22741 gtgcatcttt taatgtttct gaatctacaa cattatattg aatatggaac ccaccttttc
    22801 ttaaataact tcttgttaaa tctaataatt ttttagttcc ttgttgtcct tttatagtag
    22861 ttggatgaag ttttaaattc atctgagaat tttgaactac tgcttggtcc caaatagttg
    22921 cagattcaaa taatgcatat actccatttt tatctgttcc tgcatatgca gagactgaac
    22981 catctgaata tgttgttcca cttagtctac catctggagt agccaaggta gcagctccct
    23041 gtggcccatg tgtagaaaca gatatttggc aagggtacat cttcttagca tataaagatt
    23101 gtgcttcttc acatactttt gataaccata cctcatagtt tttaagaata ttatctgcat
    23161 acaaatcatt atttccatat ttaggtgcat caagacaggc cttataaata gcatcatatt
    23221 taccagtctt atctactttt acttggtctg ccatagaata atttcctact tctaatgcat
    23281 ctttaaatcc aaaattattt aatatggcat ccttcatttc ctctattgta aatgctttgt
    23341 catcatatac aagtttctta agtgctgcaa atgaattgac catagtaaca gtaccacaag
    23401 tttctacgtt taatgttgca ttataacgat atcccatatt tcctatatgt tgacctttgt
    23461 ctaaacagtc tggctttagt agtgaattta ttatagaagt atcaaatttt ctccatatat
    23521 ctaactcaat gttatttgct ctttctaaaa catttattgc ttgtttataa taatctttaa
    23581 atacttctat gacttcttca taagtatcta atggcttatt atgaggctca aatacttgta
    23641 tattcatacg atagtcttta ccattcataa gaacaagttc taaaattttt ggattagcta
    23701 taaagtgaac tccagagcca gcagattgac ctgctccacc tgcaatagag tatgtttttc
    23761 cgtttaaagt taactgtttc caacaccctg gagacgtttc caaacatcca cctaatgcaa
    23821 atgctcttgc ttcttcaaca gtcatacctt catcagcaaa ttgtttcatc ataaatgttg
    23881 ttccatttga gttgtttacc catgctggat aaccagaacc aagctttgta cattcagcag
    23941 ctttcattaa gaaatcttct ggaagctttt catcatatag catagttaaa ctaggttgag
    24001 gagttctaca tctcatactt gcttctaata ataactcttc tagttcatta gctcctgtag
    24061 aaccatcttc ttttaatcca cctatagata aagtattaaa tgtattgcct gatagtactc
    24121 ctccatttac gcccgctgaa gcaaaacagt caatacaagt gatttttatt ctataaagct
    24181 ctaataattc gataacctct tcttttgtaa ttagtccttt ttctatatct tgttcatacc
    24241 aaggatataa aatttgacca aaacgtccta tagacatacc agatatagca tcctcattta
    24301 ctgatgctat atgaattgta taagtcaact gaagagcttc cctaaatgta cgaggttgtt
    24361 tatgtgcaat atgttctaat atctccacta aatctgagta ttctttttta gcttctaaat
    24421 ctgtctctat actttctaaa tattttgcat gttttgcata atttaaaatc caagtttgta
    24481 atccttcaat aacttgaata acagcctcat aaaaatatag acggtccata ccaataagcc
    24541 catctccaga tgcatttcct gcaactgcct ttttacattc ttttgccatt tctataattc
    24601 catctaatcc ataatttaaa ggataaaaat aatttataac ttctctacct tgaggaagtg
    24661 tataccctga atcaaacatg catataagtg tactcattag gttttctttt aaatcataat
    24721 ctggcatcat tttttcatag tgaaaaccta aatcttcaac agactttcca acccattcct
    24781 ttgccatttt tacaagtaca ggtacttctt cttttctcat tccgaattta ccagctattg
    24841 aaacgacatt tccaaaactt tcagtaacat ttcctccacc agacccaaat tttgtaagtt
    24901 catcagctgt gttactggct aatttctttg cctcttctct catttgttct ccttgagcaa
    24961 caaagaaact ttcacttacc catggcattg gaaaagaacc tctataatgg cgtgtctttt
    25021 gcattactat tttctcacca ggtattatat ttggtgtcat atgtgaaaaa gcagctttaa
    25081 gagctttagc tcttctaact actggtatat caccatctga ttttctatat tctctattat
    25141 accaatatgg aaattccata tcagcagtag ataatgtatt ataatataca tctaataagt
    25201 cttctgctct tttacttgct tttttttcat taaattcact taaattttct ttattttgaa
    25261 aatttgattt aatatttttt gcttccaaaa tacttcttat tttgtcttct ttactttgac
    25321 tcatttcttc ccctccttaa tctttctata attagtataa aactttttaa gaatgaaaaa
    25381 aaagggtata ttacccactt tttaaaataa tctttctata gaaaaaatta aacttgaagc
    25441 aaatctaagc caaagacaat ctgtatatcc taaaatttgt tgcaattcat tggaatataa
    25501 agattataaa tagttttact tcaagtttta atatatacta tatttatttt tattatattt
    25561 aatttcactt ctacttactt ttttgaaaca atacttttat atttacaaat tcatctaact
    25621 ctactgattt atctttaaat ttggaactgc atttaaactc atatcatgcg ttttcccatt
    25681 tataaaatta taatatcctg cacaccctat catagctgca ttatctgtgc ataaaattag
    25741 aggaggatac ttaacagtaa taccattgtc tttagcaagc tctgttattt tagctctaag
    25801 ccccgaatta gaagcaaccc caccagaaag agttatgata ttgtatcctt tgtcttttac
    25861 tgcttttagt gctttagtag ataaaacctc tactacagcc tcttggaaag atgctgctac
    25921 atcttcaact actatttctt catttttcat tctttttcca tttagataat ttaatacaga
    25981 agattttaat ccactaaaac taaaatcata gctatcttct tctaaatagg ctcttggaaa
    26041 ttctatagca tgtttatttc cattcttggc cagattatca ataataggac ctcctggata
    26101 gcctaaattc atagctcttg aaatcttatc aaaagcttct ccagatgcat catctcttgt
    26161 ttttcctaaa atttcatact tgccataatc ttttacttca accaagtgag tatgacctcc
    26221 agatactatt aatgttataa aaggaggttt taaatcctta tgctctatat agtttgcact
    26281 tagatgccct tctatatgat ttactcctac aagtggaata ttcaaagtat atgcaagtgc
    26341 tttagcataa gatagaccaa ccaataaagc acctactagc ccaggtccat atgtaactgc
    26401 gatatggtct atatcattaa aacctatatt tgctttgtct aaagcttctt gaacaactat
    26461 atcaatattt tccacatgtt ttcttgaggc cacttctgga actacaccac caaatttttt
    26521 atgtgtctcg atttgagtag agattatgtt tgaaagtatt tctctaccat tttttaaaac
    26581 tgatgcagct gtttcgtcac aactactctc tatagctaat gttattatat cactcataaa
    26641 ctctacacct cttttaattg gttccacatt ataattgcat cttctttatt atcactgtag
    26701 tattcttttc ttattccacc cattttaaat ccatattttc tatataaatt ttgtgctatt
    26761 ttatttgatg ctctaacttc taatgtcata gatgcaatat tattatcttt acataaatca
    26821 actatgcctt ttatcaactt gtcccctatt tttttccctc tatagtcact atgaacagcc
    26881 acattattta tatgcccttc atcaagtata agccaaattc ccacatatcc gacaattttt
    26941 ccatttaact tagctactat ataccttgct acttcattag acaactcttt tctaaaagac
    27001 tcttttgacc aataatcttc aaaacaattt ttttctactt caaaaacttc atctatatct
    27061 tctgtagtca tttcttctat ttttatatca tctactatta gttttgtatt atcttccatt
    27121 atttagcctc ttcatttttt catcatattg tacttcagct tgagattttc tgatatacat
    27181 aggatttatc gtataacaag tatgtacatc tatattttta ttatatttat ttaatgctaa
    27241 agagcataag cttcctgcgt tagatacatt attagctggt gaaggtattt ttatattttt
    27301 tatattctct aacttgtctt tatatttgta tacagcttct ccaactatta taaattcttc
    27361 attagttgat acaatttcat ctactaaagc atcaaactct acaacatcaa caccatctaa
    27421 tcctatcatc tctccatttt caaacttgta tttagctgaa taaacttgat ttttctgcgc
    27481 atctaatata caacatatct ttctatcaca gaaatttatg ttatttgcta gagattccaa
    27541 agaatttact gctattattg gtatattatt tacatgggcc atagctttta ctgtagccat
    27601 acctattcta agtcctgtaa acgaacctgg acctatacat acagctaata aatccatatc
    27661 ttttatactt aaatcactca tactcaacat attttctatc attggcataa gcttttgtga
    27721 atgagttttt ttgttgttta ctgtaaactc acatattaag ttatcatctt caacaactgc
    27781 tacacttgct gccatagaag acgtatccat acctagtatt ttcattattt aatcaactcc
    27841 tcaactatct ctttataaaa ctctcctttt ggagttaata tcatttctct tccttcatct
    27901 ttatatctca attctatatt caaatattct tttggaagta tatcttctat tagattagcc
    27961 cattctatta tacagattcc atttgaattt acatattcat cataaccaat atcatacatt
    28021 tcatcactac tacctattct ataaacatca aaatgataca aaggtatttt tccttcatat
    28081 tcatttatta tagtaaaagt aggacttgtt atataatctt ctatccctaa tgaatctgcc
    28141 aaactttgtg tcattgtagt tttaccagct cctaaatctc ctactaaaca gataacactg
    28201 ccttctttta aaagtttacc taatttatat cctatttctc tagttttatt ttcattttct
    28261 aaatatattt ttgccatcaa ttcatctcca ttttgatata atcgtcatgt gaatttatat
    28321 cactgcttaa ttaattatag tataagccta gattattata aagtacttgt ctttatcttt
    28381 ttttatcttt cactattgat ggctgtatct acaccttata tacaatcatc aatagcaatt
    28441 tttacatact cttttgcttt atggttttgc ttcaatgctt ctttacatat tctctcagat
    28501 tgctctttta catacttaag agacttatag ttctctttta ctgcttcaat acacattttt
    28561 tctgtttgat tttttatata cataagcatt tttccatttt gtcttacaat ctctatacat
    28621 atatcttctg tatacttatc tatgtaactt agtacatctt taatattata ttttttattt
    28681 aaatatctta tagcatttat acatatttct tctgtcttat tgttgacaaa ttgtatactt
    28741 cttccatctt gtttgacagc ttctatacat attttatcag tttgtttatt tacatattgt
    28801 aatgccatac cattttgtct tacggattct atacacattt tttccgtttg attttttaca
    28861 tattttaatg ctttataatt ttgcttaatg gcctctatac ataattcttc actctgttct
    28921 ctaacgtatg caagacacct gccatctttt ttaattgctt ctaaacatat ccttcttgtt
    28981 tgagtctcta aagcctttag tatacttaca acatctatat tggaagaatg acttattgct
    29041 tttataataa gcccttctgt tttatttcgt acatacttaa gtgcattaca atcttttttt
    29101 atagcttcaa gacacaagta ttctgtttgt tcttttacat attcaagtgc tttataatta
    29161 tactttattg cttctaaaca taattcttcg gtttgatttt ttacatactc taatgccttg
    29221 taattctgcc ttattgctaa aatacacata ccatcagttt gattttctat attttttaat
    29281 tcacgataat cgtattttac cctttccata ttttcaatat attcattagt gatatacttt
    29341 aaagaatctc ctaattttgt atcataaata ccaatatgaa tatctttcca attactatta
    29401 aaataatacc ctttatcatc taaaaataat ttttttattt ttttagataa atgcatgaat
    29461 atacacccct atcattatta aattagttat tattaaaagt ttgattatta atctttatag
    29521 ttttttaata ataactttat ttttt
//
LOCUS       DAEORZ010000038        29474 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEORZ010000038 DAEORZ010000000
VERSION     DAEORZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.038"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            63..977
                     /gene="murB"
                     /locus_tag="KRH39_001539"
     CDS             63..977
                     /gene="murB"
                     /locus_tag="KRH39_001539"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBG8511453.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            981..1694
                     /locus_tag="KRH39_001540"
     CDS             981..1694
                     /locus_tag="KRH39_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG8511454.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRYFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            1725..2582
                     /gene="rapZ"
                     /locus_tag="KRH39_001541"
     CDS             1725..2582
                     /gene="rapZ"
                     /locus_tag="KRH39_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBG8511455.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            2594..3700
                     /locus_tag="KRH39_001542"
     CDS             2594..3700
                     /locus_tag="KRH39_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBG8511456.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVEHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            3769..4194
                     /locus_tag="KRH39_001543"
     CDS             3769..4194
                     /locus_tag="KRH39_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG8511457.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            4211..5158
                     /gene="whiA"
                     /locus_tag="KRH39_001544"
     CDS             4211..5158
                     /gene="whiA"
                     /locus_tag="KRH39_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBG8511458.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            5315..8884
                     /locus_tag="KRH39_001545"
     CDS             5315..8884
                     /locus_tag="KRH39_001545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBG8511459.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNSIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            9093..10052
                     /gene="pfkA"
                     /locus_tag="KRH39_001546"
     CDS             9093..10052
                     /gene="pfkA"
                     /locus_tag="KRH39_001546"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG8511460.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            10104..11864
                     /gene="pyk"
                     /locus_tag="KRH39_001547"
     CDS             10104..11864
                     /gene="pyk"
                     /locus_tag="KRH39_001547"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBG8511461.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGDACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            12039..13397
                     /gene="rlmD"
                     /locus_tag="KRH39_001548"
     CDS             12039..13397
                     /gene="rlmD"
                     /locus_tag="KRH39_001548"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG8511462.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIQDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKNTNAILGRENIVIHGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVEIVAKII
                     KQ"
     gene            complement(13863..14360)
                     /locus_tag="KRH39_001549"
     CDS             complement(13863..14360)
                     /locus_tag="KRH39_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511463.1"
                     /translation="MKKRKIVIITLLLIGVLLFGGILYNLFLVKTANISMLKESWNLD
                     IPIPNKEIEVFDTQDSINGDGQSYFIQEFSEKNFKKVFNLKGGIVVSKDNINEIEKYI
                     DKFKRDSVSINKSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYS
                     LIWNQ"
     gene            complement(14687..14863)
                     /locus_tag="KRH39_001550"
     CDS             complement(14687..14863)
                     /locus_tag="KRH39_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511464.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(14917..15013)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            15809..16210
                     /locus_tag="KRH39_001551"
     CDS             15809..16210
                     /locus_tag="KRH39_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBG8511465.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGYLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLSQPPFPLERIIEKLEKEETE"
     gene            16207..17274
                     /gene="rsgA"
                     /locus_tag="KRH39_001552"
     CDS             16207..17274
                     /gene="rsgA"
                     /locus_tag="KRH39_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG8511466.1"
                     /translation="MNGMRQINLQDYGLNRNFITQSMGYHDLYLARIISQEKNFYKAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            17810..18316
                     /locus_tag="KRH39_001553"
     CDS             17810..18316
                     /locus_tag="KRH39_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511467.1"
                     /translation="MKISSQYRSQYSFRHESNVNNTRINESIVKKNEIVGKDTYLSNI
                     MKQKQELNDRIRDLKDRQEIYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNRIIED
                     KNKSTMLDENSTYLNTNDEKESLIAKEFNEKVEEEILNDEKLEELEQKRIIKRILIKK
                     RRYLRTYP"
     gene            18346..18507
                     /locus_tag="KRH39_001554"
     CDS             18346..18507
                     /locus_tag="KRH39_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511468.1"
                     /translation="MLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIFDKTDE
                     QKKHINVLV"
     gene            18551..19099
                     /locus_tag="KRH39_001555"
     CDS             18551..19099
                     /locus_tag="KRH39_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511469.1"
                     /translation="MEGLGFYAAALAGGSYQRIGFGKLNPIEVIADGDWISYKQAQDT
                     LTVIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLLDKRGVCGSFA
                     SSFHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGTLDFDYCKLK
                     DATREMTTYYDAKALSILGFNQ"
     gene            complement(19411..20367)
                     /locus_tag="KRH39_001556"
     CDS             complement(19411..20367)
                     /locus_tag="KRH39_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG8511470.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRITDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSKILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            21070..22065
                     /locus_tag="KRH39_001557"
     CDS             21070..22065
                     /locus_tag="KRH39_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8511471.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            22043..22906
                     /locus_tag="KRH39_001558"
     CDS             22043..22906
                     /locus_tag="KRH39_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG8511472.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTIIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            22903..23697
                     /locus_tag="KRH39_001559"
     CDS             22903..23697
                     /locus_tag="KRH39_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG8511473.1"
                     /translation="MIKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            23982..25361
                     /locus_tag="KRH39_001560"
     CDS             23982..25361
                     /locus_tag="KRH39_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8511474.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIC
                     KYKNRYKYM"
     gene            25811..26524
                     /locus_tag="KRH39_001561"
     CDS             25811..26524
                     /locus_tag="KRH39_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8511475.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLTNDGFKNIIKAYNGLDAI
                     SLCKITCPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIVFRVKAQLRRQQYQSIVPSDSEVIKVGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(26802..28340)
                     /locus_tag="KRH39_001562"
     CDS             complement(26802..28340)
                     /locus_tag="KRH39_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG8511476.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGAGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINKDTTISLYLHTAFEKLYKIEKEMN
                     LLYQKSAISGNEQHLIKASDYQEQLIANNFYYIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSDYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEEIFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            29222..>29474
                     /locus_tag="KRH39_001563"
     CDS             29222..>29474
                     /locus_tag="KRH39_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HBG8511477.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTI"
BASE COUNT        11233 a         3275 c         5068 g         9898 t
ORIGIN      
        1 atactaatgt aatttaaata ataatgacag ctaaagttca gtataatttg ggaggaaata
       61 caatgaataa tcaagatata tatataaatt tattaaatat attaagtaaa gaggatataa
      121 aagtcgatga acctatgaaa aaacatattt catttagggt aggaggacca gcagatatat
      181 tagttagacc tagaactgaa gaacaattaa aaaatgtact taagttggta aaagaagaat
      241 ctattccgta tttaataatt ggaaatggtt caaacatttt aattaaagat ggtggaataa
      301 gaggagtggt aatagaatta gctgataatt tcaattctta tgagataaat gatactagga
      361 tgactgctca gtcaggagct ttattatcag ttttagggaa agctctccaa aagcaagaat
      421 taaaagggtt tgagtttgca tcaggaatac ctggcacatt gggaggagca ttagccatga
      481 atgctggagc atatggtggt gaaatgaagg acatagtgaa gtcagtaaga ctgatggata
      541 tggaaggcaa tatatttgaa ttatcaaatg aacagatgga atttgggtat agaaaaagta
      601 taatatcgaa aaacggatat atagctttat cagcagaatt agaattacaa gaaggaaatt
      661 atgatgaaat aaaaagttta atggatgatt tagcgacaag aagaataact aaacagccat
      721 taaattttgc tagtgcagga agtacattta agagaccaac aggatatttt gcaggtaaat
      781 taatagaaga aactggattg agaggtctta ctttaagagg agcacaagta tctgagaagc
      841 attgtggatt tgtagtcaat caaggagaag caagtgctaa agatattttg gatttaatat
      901 atgttataaa aagtgctgta tatgccaagt ttggggttat gttggaagaa gaagtcaaaa
      961 tacttggaga ggattaacaa atgaaaatac tggctgatta tcatacacat acattatata
     1021 gtcatggcaa agggactatt gaggataatg taaaggtagc catttctaaa ggaataaaaa
     1081 caataggaat atctgaccat agttataaac atattgcata tggtgtaaag aaaaaagata
     1141 ttctcaaaat gagagaagaa gtagattatt taaatgataa gtacactgat ataaatatac
     1201 ttttaggtat ggagtgtaac atattagatg ataaaggaaa tatagatatg gatgataaat
     1261 ttattagata ttttgattat attatggctg gatatcactt tggttctact ccaacatcat
     1321 taagaagctt attaaatcat tgtaataact atatttttaa aactgaaaaa gcaaaagaat
     1381 ataatacaaa agctgtaata aatgcgataa gtaataatga tatttttata attactcatc
     1441 caggagataa aggcgatgtg tatatagaag aagtagcaaa gacagcaaaa aagacaaata
     1501 ctagacttga gataaatagt agccattctt ttttgaatgt aaatcaatta aataaaataa
     1561 aagggctaga aaataaattt ataataggct cagatgcaca ttgtccagaa agagttggtg
     1621 attttgaact agctatgaag actatatctg actctaatct ggatatatcg ttagtagaaa
     1681 acattataat ataataaaca taagataaaa gggaggagtt agcaatgaaa tttgttatag
     1741 ttacaggtct ttcaggatct ggaaaaagtg agacaatgag agctttagag gatatgggat
     1801 tttattgtgt agataatcta cctcctgctt tgataactaa atttgcagaa ttatgttatc
     1861 aaccaaattc aagtattgat aaagttgctc tagggataga tataagaggg agaaagtttt
     1921 ttgaagcgct tcatgaaagc ttaaattatt tagaaaaaga aaattatgaa tatgaaatgg
     1981 tatatttgga ttgtaatgat gatgtattgt taaagagata taaaatgaca agaagaaatc
     2041 atccattggc aaaagatatg cagattccag aaggtataaa aatggaaaga aagataatgg
     2101 aacctttaaa ggagttatct acatgtataa ttgatacaac taacatgaaa cctaaagacc
     2161 ttaaggaaga aataaagaaa atatattctt ctggtgaaga taatccaaac ctaactattt
     2221 ctgtagtatc ttttggattt aaacatggta tacttgctga tgcagattta gtatttgatg
     2281 taagatttct tccaaatccc tattatgttg aagagttaag agctaaaact ggtgatgata
     2341 aagaagttag agattatgtt atgaattcta aaatcagtga agaattttat gtgaaacttt
     2401 tagatatgat tcatttttta gtacctcaat atatagagga aggaaaacaa catctagtaa
     2461 ttggtgttgg atgcacaggt ggaagacata ggtcagttac tataacaaat cttatagcgg
     2521 aggatttatc taataaggga tatagagttg taaagaaaca tagagactct atgttgcgat
     2581 aatggaggca aacatggcta aattacttat tattgtagga attttaggtt ggattgcatt
     2641 aattgtatcg ttatttatat ataaaaaagc aaaaaatgaa aaagtaaaac tatttcaaag
     2701 gtctattgtt gaaaatagag aaccaaatgt ggttgtgata ggtggaggta ctggtcaatc
     2761 ggtattttta agaggcctta aacacactac gcaaaatata actgcaattg taactgttgc
     2821 tgatgatggt ggaggttcag gtgtattgag agaagactta ggaatgctcc caccgggaga
     2881 cataagaaac tgtttattag cacttgcaaa tatagagcca acaatgaatg aagttatgca
     2941 atataggttt acagaagggc ttttaaaagg tcagagtttt ggaaatttgt ttttggctgc
     3001 tatgaatggg ttgtatggaa actttgaaaa agctgtatat aaattaagtg aaatttttgc
     3061 tataacagga agagttttac cagtaacttt ggaagatgta aatttagttg ccaaattaga
     3121 aaatggcaat ataataaatg gagaatctag tatacctgaa gagtcaaaaa ttcaaaaaag
     3181 ctctattgat aaaatttttt taaatccaaa agatgtaaaa ccattaaagg atgttattgc
     3241 atctatttat gatgctgaca ttataattat gggtcctgga agtttatata caagtataat
     3301 acccaattta cttgtagagg ggattgtaga cgctattaaa tcgtctgtag ctccaaaagt
     3361 atatatagca aatataatga ctcaaccagg tgagacagaa gggtacaatg tattagagca
     3421 tgttaatgct atagtagaac acactgatga gaatttaata gattatgtaa tcgcaaataa
     3481 tgagatattg ccagaagaaa tgcttgactt atataaacaa gatggagcag agcaagtctt
     3541 gttagataaa aagcaaaaag ataaattaaa agagatgggt ataaaaactg tagagaagaa
     3601 tttaattgaa ataaaaaata attatataag acatgatgca aagtatatat ctaacatagt
     3661 tattgagtta gccctaaatc ataattacaa caaaagttag taaaaaagga ttttaaaaaa
     3721 ttgtgtagaa atccttatca ttatgttagt tttaaggagt gaacatcgat gagagaagag
     3781 gttagtgctg gtggtgtagt cctgtttggc aatacgattc ttttactaag gaagttcaat
     3841 ggagattggg tgttacccaa gggaaaagta gaagaagggg aaaataacca agaggcagca
     3901 ttacgtgaag taagtgaaga aactggagta aaagcagata tattgaaata tttaggtgaa
     3961 atacactata cattcaaaga aaactgggat gaaaatagag cagttcataa aacagttttt
     4021 tggtatttga tgcaagcaaa gaatatggat actattccac aaaaagagga aggctttatt
     4081 gatgctaaat ttatccacct agatagagta gtagacttag ctaggtatga tgatgaaaag
     4141 gagataataa aggttgcctt acaagaaata aaaaaaaggt taaagaaaaa ttagttaata
     4201 ggtgatagat atgtcttttt caactgaaac caaaaatgaa ctagcaagag ttatatctgg
     4261 gaatgaatgt tgtaatatag ctgaattgtc agctctagtt aaatcagggg gaagtataca
     4321 aattgttgga tataagaagc taaatctaaa gataacaaca gagttaaatt caatagcccg
     4381 taaagtattt aaattattaa aaaagaattt taatatcaat actacaatct cagtaaataa
     4441 gaatcaaatg ctaaagagaa ataacagtta tgtattaatg gtaacaagcg aaatgggttc
     4501 agaaatgtta ttaaaaaagt taggtatact tgaagataaa gaaggatttt ttactataaa
     4561 caaaatttca gagaacttaa ttaagcatga aggatgtaag agagctttca taagaggagc
     4621 ttttcttggt ggaggttcta taagcgaccc agaaaagaac tatcatatgg aatttgtaac
     4681 taataatgag gagtttgcag attctctaaa agaacttata aactctttag gttttaatag
     4741 taaaattgtg gctagaaaaa ataatcacgt tgtttatatc aaagagagtg aacaaatatc
     4801 agatttgttg agtattatag gaggacacca tgctctttta agtcttcaaa atactaaaat
     4861 agtaaaagaa atgagaaata atgtaaacag gatagtaaat tgtgagacag ctaacctttc
     4921 taaaacagta aatgcagcag taagacaagt agaaaatatt aagctcatac aagagactat
     4981 aggaattagc agtttgccag aaaatttaca agagatagct aagattagaa tcgagtatga
     5041 ggatatgact ctcaaagaac ttggagagat gttagaacca ccaataggaa aatctggagt
     5101 caatcataga cttagaaaaa tagaagaaat tgcaacagat ttgaaaaaaa aatcatagca
     5161 ataaattgag gggacaatct cagaataagc attaatttat aacatataat ggtatatgtt
     5221 gaagttaatt cctattttga gataaaagtc tctgtttttg ttttagtaca tatatttgtg
     5281 gtaaaattaa aattataaag caggaggtga tttaatgaac aaagattttg ctcatcttca
     5341 tgtacatact gagtatagtt tacttgatgg gttttcgagg gtgaaaaaac ttataaaaag
     5401 ggctaaggaa ctaaacatga gctcaatagc tataactgac catggttgta tgtttggtgt
     5461 tatagatttc tacaaaacag ctataaaaga aggaataaag ccaattatag ggtgtgaagt
     5521 ctatacagct gcacgtggat tgagagataa agacccaaac tatgacaaat atcaaggaca
     5581 tttagttctg ttagctaaga atatggaagg atataaaaat ttaataaaaa tagtttcaac
     5641 ttcatacgta gaaggttttt actataagcc tagagttgac atggaagaac ttaaaaaaca
     5701 taacaaaggg attatagcac tttctgcatg ccttgcagga gatgtagcac gtgctttaat
     5761 gaatagaaat tatgaaaaag caaaacaatt tgctcttgac tacagagaca tatttggaga
     5821 agaaaatttc tttttggaga ttcaagatca caatttacct gagcaaaaag aagtaaactc
     5881 tggtttagta aaattatcaa aagagactgg tataccacta gttgctacta atgatgttca
     5941 ctatgtaaat aaagaagatt cgaaaataca tgatgtactt atgtgtattc aaatgggaaa
     6001 aacattaaat gacccaaata gaatgagatt tggtagtgat gagttttatt taaaatcaag
     6061 agaagaaatg gaagaattat ttccatatgc attagaggct attgacaata ctgtgaaaat
     6121 agctgatatg tgtaatgtgg agtttgattt taatactata catcttccta agtatgatgt
     6181 tccagaagga tatacaccag aaacttattt aagggagttg tgttttaatg gattaaaaga
     6241 gagatatgat aatcctagca atgagatact agagagatta aattatgaat tagatgtaat
     6301 tgaaaaaatg ggttatgtag agtatttttt aattgtttgg gattttataa atttttctaa
     6361 agaaaataat attatagtag gaccaggtag aggtagtgcg gctggttcga tagtagcata
     6421 tacacttaaa ataactgata tagacccaat aaagtactct ctattatttg agcgtttttt
     6481 aaatccagaa cgtatatcca tgccagatat agatatagat ttttgttatg agagaagaga
     6541 agaagttata gattatgtaa agagaaaata tggtgatgac catgtagccc agataataac
     6601 ttttggaaca atgggtgcaa aagctgcaat aagagatgtt ggaagggtat tggatatagg
     6661 ctacaataaa gttgacaagg tagccaagga aataccattt gctttaggaa tgacaataga
     6721 taaggcccta gatacaaatc ctaatcttaa agaattgtat gaccaagacc cggaaacaaa
     6781 agaaataata aatatatcaa aacagataga gggaatgcta agacatgctt ctactcatgc
     6841 agcaggagtt gttatatcaa aaaatcccgt agatgaatat gtacctttgt acaaacatca
     6901 agatgcaatc acaacacagt ttactatgac aacacttgaa gaacttgggc ttttaaagat
     6961 ggattttctt gggctaagaa ctcttacagt tataagagat actcttgatt taatagaaaa
     7021 aaatagagtt ataaaaaatt acacagaaaa aatagacttt tcaaagatgg attatgacga
     7081 cccgaaagta tatgaaatgc tttcatcagg aaatacacta ggggtgtttc aattggagag
     7141 tgctggcatg agaagtttta tgaaacagct aaaaccagac aattttgaag atattgtagc
     7201 gggaatatcc ctatttagac caggtccaat ggattctata cctgcatata taaaaaataa
     7261 aagcaaccca aaagatgtaa catatctaca tgaaaaatta aagcctataa tggaagttac
     7321 atatggttgt ttggtatatc aagagcaggt tatgcaggtc gttagagatt taggtggtta
     7381 tagttacgga cgaagtgacc tagttagaag agccatgagt aagaaaaaga tggacgtgat
     7441 ggaagaagaa agacaatatt ttattcatgg taaatttgat gaagatggta atatagaaat
     7501 tgcagggtgt atcagaaatg gtgtagaaga ggaaatagct aataaaatat ttgatgatat
     7561 gatagacttc gctagatatg catttaataa atcacatgca gctgcatatg gggttttagc
     7621 atatgagaca gcttatctaa aaacatatta tccagttgaa tttatggcag cattgataac
     7681 cagtgtaatg ggaaatactg ataaggtagt tgagtatata agagaatgta aagcactaga
     7741 aatagatgta ttacctccag atataaacaa aagtttttca aagttttcag ttgaaggcga
     7801 caatataaga tttggtctag cagctgtaaa aaatgttggt gtaaatataa taaacaatat
     7861 aatcgctgaa agagaatcta atggattatt tgtagattta gtagatttag tcaaaagatt
     7921 agaccaaaaa gatacaaata agagagtaat agaaagtcta ataaaatgtg gagcttttga
     7981 taatataagt gaaaatagag ctagtttgat ggctggttat gaaagtttat tagaaagtgt
     8041 atctatggat agaaagaaaa atgttcaagg tcaaatttct ttatttgatg cttttggaga
     8101 tagtgaggaa gatagtgatt ttcaacaaat atacagttta cctaaaaggc aagagtacga
     8161 agaaagagag agacttaacc ttgaaaaaga agtcttggga atgtatgtaa gtggacatcc
     8221 tttatcacaa tttgaaaaag aactacaaga aaaaacatct atggataatg gaaaattaaa
     8281 ttcattaaaa gaggaagaag agtcatacct acaaatgaat gaaacagatg ttataatggg
     8341 tggaatgatt gtaactaaaa caattaaaac aacaaaacga aatgaaatta tggcatttat
     8401 tgaattagaa gatttgtatg gtgctataga aataatagtt tttcctcaat tattacaaaa
     8461 gtataatgtc attttgaatg aagataatat aatatatgta aaaggaacat taagtataaa
     8521 agaaggcgaa aatgcaaaac ttatagctag agagataaaa gatataaatg atgatactga
     8581 ctttgcagat agaaactaca attcaggaag aactagaaat gtagaaaata ttaagccaga
     8641 aagtggtaaa aaattgtatt tgaaaataga tagtatgtct gatactgata caactaatag
     8701 tattgtagaa atagccaaac aatatccagg taatgacagt atatacttgt accctatgga
     8761 tgaaaatata aatggtaaac gtaaagcata taaaatgacc ggggcattga cttgtatatg
     8821 tgatgaattt ataaataact tagaagagtt acttcctaaa gataacataa aagtaaaagc
     8881 ttgatttttt atataaaaat ctaaatatat tataataata tgaaaaatat tgtccgtttt
     8941 gtgatataat atcacaaaat ggacatttaa ggacatttaa tttttttagt cttgatggga
     9001 ttaaaaagtg atatagggac aaaaaaagtc cattagaata caataacaat gattataata
     9061 aaaaattgac ataaaatcca aggaggtaaa aaatgaaaac tataggatta ctaacaagtg
     9121 gaggagatgc tccaggaatg aatgctgcga ttagagcggt tgtaagatca gcaatatatt
     9181 atggatgcaa agtatatgga atcaatagag gatataaggg attactagaa gaagatttaa
     9241 ctgaaatgaa cttatcttct gttggagata ttatacacag gggaggaact atattaaaat
     9301 cttccagatg tgaagaattt aaaacagaag aaggtagatt aaaagcagta aaaattctta
     9361 aaaagtataa aattgattgc ttagttgtaa ttggtggaga tggttcattt gcaggagctc
     9421 aaaaacttag tgacttagga ttcccagcta taggcatacc aggaactata gataacgatt
     9481 tagcatatac agattatact ataggatttg atactgcaat gaatactatt atagatgcta
     9541 taggtaagat aagagataca tcatcatctc atgaaagagt taacatagta gaagttatgg
     9601 gaagacactg tggagattta gccttatatg caggtcttgc aggaggagcg gaaactataa
     9661 tagtaccaga agtagaaatt acagtagatg aagttgctct aagattaaag actactcaaa
     9721 aaagaggtaa gagacatagt ataatcgttt tagcagaagg agtcggaagt gctagtgatt
     9781 tagaaaagga acttaaaaaa gagtcaggag cagatttaag agtcactgta ttggggcatg
     9841 tccaaagagg aggaagccca actgtatctg atagaatact agcaagtaga cttggagtaa
     9901 gagcagtaga attattatta gatggaaaat cagctagagt tgtaggaata aaagaaaaca
     9961 aaataattga tttagaaata agtgaagctt tagctcaaaa gaaagtattt gataaagaag
    10021 cttatgagat ggcaaaaata ttatctatat aagttttagt attaaaaata aatctgtgga
    10081 ataatatctc aggaggagca aatatgttaa ataatgtaaa gaagacaaaa attgtttgta
    10141 cactaggtcc agcatcacaa agtgaggaag ttttaactca acttatgcaa aatggtctaa
    10201 atgtgtgtag atttaacttt tctcacggtt cgcatgaaga acataaagaa agaattgata
    10261 tggcaaagaa agttagagaa aaattaaata agccagttgc tatattatta gatactaaag
    10321 gtccagaaat tagaacaggt aactttgaag acccagaggt atttttagaa gaagggcaaa
    10381 agttcactat aactatgaaa gatattatgg ggactaaaga aatgtgtaca gtaagctata
    10441 aaggtcttgc tgaagacgtt aaatcaggag attctatact aatagatgat ggtttagttg
    10501 gcttaagagt aaaagaaata aatggagaag atatagtttg tgttgttgaa aattcaggaa
    10561 tagttaaaaa tcataaagga gtaaatgtac ctggtgttaa gataaattta ccagcaataa
    10621 ctcctaaaga tataagtgat atagagtttg gaataagtca aggaatagat tatatagctg
    10681 cttcttttgt aagaaaggca tctgatgtac tagcaataag agaagtatta gagaacaata
    10741 atgcaactga tatacaaata atatctaaaa tagaaaacca agaaggtgta gagaacctag
    10801 atgaaatatt aaaagtttct gatggtataa tggtggctag aggtgactta ggtgttgaga
    10861 taccaactga agagatgcca atagttcaaa aaatgatgat aaaaaaatgt aatgaattag
    10921 caaagccagt tgttactgcc actcaaatgc ttgattctat gataagaaat ccaagaccaa
    10981 caagagcaga agttacagac gttgccaatg ctatatatga tggaacagat gcgataatgt
    11041 tatctggaga aactgctgct ggaaaatatc ctgtagaggc agtaaaaatg atggctacaa
    11101 tagcaaaaag aacagaagag actttagatt atgatagatt attaaaggaa aatggtacta
    11161 acaatgttac tgtaacagat gcaataagcc atgcaacttg tacaacagca gtagatttaa
    11221 atgcatcagc tataataaca tctacttctt caggatatac tgctagaatg gtatctaaat
    11281 ttagaccaaa atctccaata atagctacta caaataatga aaaaactatg aataaattag
    11341 ctcttacatg gggagtatat ccagttaagt cttctgttgc aggaaataca gatgaagtta
    11401 tagaaaaggc aattgaggca gctagacaag ctgattatat agataatgga gaattagtag
    11461 ttataactgc tggagtacca gtaggagtaa gtggaactac taatttaata aaggttcatg
    11521 taataagcga agaaatcgtg caaggtatag gtgtaggtac tcaaacagta gaaggtaaag
    11581 ttcgtataat aaagaatgga gatgcttgtg tagactttaa tgaaggagat atacttgttt
    11641 ctactgctac tgatgctgaa atgaacaatt atatagaaaa agcaggagct gtagtaactg
    11701 agaatggtgg aatgacatca catacagcta ttgtaggtat aaatttagat ataccagtta
    11761 ttgtgtctgc aactgatata acaaatctag taaaagatgg agaagttgta actgtggatg
    11821 catcaagagg tgtggtatat agaggttcaa ctagggtttt ataagaaaaa taataaattt
    11881 atttactaag gggctttata agctcctttt tttttggtaa aataaaatat tgtagcttag
    11941 taaaaaatat agtttaatta atagcaatat tttaatataa atttaaagaa aaaagtttgt
    12001 aaagatttaa atataataga ttgattggag ggtgatttat gctttcgaaa aacgagatgt
    12061 atattgtaga tatagtagac attgggcaag gcggagtagg agtaggaaag tacaatggta
    12121 ttacagtttt tgtagagggc ggattaatac aggataaagt taaagttagg attaataaag
    12181 taaagaaaaa ttatgctgta ggagatattg ttgagataat agaaaagtct ccattcaggg
    12241 ttaaaagagt gtgtagtgac aatcttaggg attgtggagg gtgtcagata caagatttag
    12301 attataataa acaacttgaa atgaaaacaa atgaagttaa acaagtactt tcgagaatag
    12361 gtaagttaga ggatgtagaa gttcatgaca ctataggaat ggataatcca tatagatata
    12421 gaaataaagc acaatttcca atacaaaaga ataatggtat tccaattatt ggcttttata
    12481 aaaaaaagac tcatgatgta atatcgacag aaaaatgcgt aatacaacat gagataaatg
    12541 ataaaattgt taaaataata aaaacatata taagggccta taaagtaagt atatatgatg
    12601 aaaagactca cacagggttg attaggcatt tagtaacaaa aataggtttt acgactaaag
    12661 aagtaatggt tgtattagta gcgaatggaa acaaacttcc atatcttaaa gaattagctt
    12721 cagtgcttaa ggaaaatata cctggattta agactttggt tgtaaatgta aatcaaaaaa
    12781 atacgaatgc tattctggga agagaaaata tagttataca tggagatgga aaaataaacg
    12841 attatatagg tgacttagtg tttgagattt caccattatc atttttccaa gtaaaccctg
    12901 ttcaaactaa agtattgtat gataaggctc ttgaatatgc caatttaaat gatactgata
    12961 ctgtttttga tatttactgt gggataggta caatatcttt atttttggca caaaaggcta
    13021 aaaaggttta tggaatagaa attatagatg atgccataaa agatgctaag ataaatgcta
    13081 aattaaacaa tatacataat gctgaatttt atgttggaaa ggcagaggaa attgtaccta
    13141 agatgtatag agagggtaag actgccaatg tagtagttgt agacccacct agaaagggat
    13201 gtgacgaaaa ggtattagag actattgttt caatgaatcc ggaaagaatt gtctatgttt
    13261 cttgtaatcc ttctacttta gcaagggact tagattatct aaatgttcac ggatttaaat
    13321 gtgttgaggc acaacctgta gatatgttcc ctcattctac acatgtggaa attgtagcta
    13381 agataatcaa acaatagtaa tgtttatata gattatatac taataatata ttgtatttgc
    13441 caccgatttg ccactgtaaa gtaattttgg tggcaaattt agtctacaat gttatcaaaa
    13501 atctctaaag ttttattttt aattctatag aacttattat aattgtaatt ttaaaagaaa
    13561 tcgtcaatct acagttgcca gattttatac aactcatttt tttcatagaa taatttacat
    13621 aatctatagt tgaatattat ttaatatttt tatattataa ttcactagta aatataggca
    13681 ttacagatgt tgataaacat gtgaaagcac atatccactt ataattatta taaaatcacc
    13741 tcttcatttg ttgtatgttt ttataacaaa tgaagaggtg atttccagac aatttaaaag
    13801 gtttttgtat attgactaaa atttgttttt attatttata attcttaata atacatatgt
    13861 acctactgat tccatattaa actgtataat ttttgttcat ctttttttat aattaaaact
    13921 acatagtttt cataatttct cttatatata tacttatcat cttttttaac ttctaactta
    13981 tattttttaa aatcttcttc aattttattt ttattacttt tattaatgct aacagaatct
    14041 cttttaaatt tatcaatata tttttctatt tcattaatat tatctttgct tacaactata
    14101 ccacctttta aattaaaaac ttttttgaaa tttttttcag aaaattcttg aataaaataa
    14161 ctttgtccat ctccattaat actatcttga gtatcaaata cttcaatttc tttgttaggt
    14221 attggtatat ccaaattcca agattccttt agcattgaaa tattagctgt ttttactaaa
    14281 aaaagattat ataggatacc tccgaataac aaaacaccta ttaatagtaa tgtgattatt
    14341 acaatttttc tttttttcat ttttcgttca caaccttttg aaaaaacatg atttaattca
    14401 tcatttgaag gtatgtcgtc aagaataatt ttgttaatct aatccttcaa acttaaaaat
    14461 catttatgtt tacagaattt cattttaaga gatgtttatt ttcatataga taagagaatt
    14521 taagttctca tatttattct ttgatacaaa tttacatctt tattatatgt attttaaata
    14581 aaaaataaaa ttatgaatac tagaaatcta tcttaattag ctttctaaca taagactatt
    14641 taaagtagat aaaactaaaa agggttctct agaaaaccaa atctatttaa ttatacttac
    14701 ttactaatac catcacctga tgcaaacaag attctactac attttctccg tactctagta
    14761 taaaatcatc tagttgtgta tctataaaat cacaataatc atatgaacta ccaatataag
    14821 cagtcaacat atgtgtacat ctatccattt tatctctaaa catatatatt attatccccc
    14881 taaaaaactt tcataaaaac aaaaagaata tctctttttg gcaactggct gacataactc
    14941 atagtttatt ttataagcgt tagtccctat agctttgcgt cactaaattt ctctagtttt
    15001 gccgatttag ttttattcta caattacaat aatacaatag aattggttat tattcaatat
    15061 acttatttga aaattaaatg aatttataaa tatgaacgtt attttatatc aaaaaaatag
    15121 gctatgtatg tagaatgtat ggaaaaactt gattgtaggt ttcgtattaa aagacctttg
    15181 ctattaattt aagttagata aattgagcga taagcataaa atgagaaaac aaataatagc
    15241 agttaatttt tattattaat actaggtaaa cattaaattt taaaatacca ctagatttta
    15301 tagaataaga aaatgagaca aacataaata tatgaatata ttaaattata cttgaacata
    15361 cttatatttt atggtaaaat ttcaataata attattagta aaggagtgtt ttgcctgatg
    15421 tcagagttat atataatggt aaccaacaaa taaatagttg gagacgcata tgggttctgt
    15481 ttaatcagaa tttttaagtg tatttttgat acatttgatt gttgtgtctt actctgcctt
    15541 tatagataac taacattaga caacaaataa taatattaca tattactttg ttaaattgtg
    15601 gtgtagtggg ctttaatctg ctacactttt attaatttta cacagtatta ttttgaatat
    15661 aattatgttg aaatagaaga tattaaattt tctagtccac aggagttttc tgtttttgtg
    15721 taggtaaata tgtataccac ataaaaaggc agatataaat acctgtggac tttttttgta
    15781 gaaaatacaa gatacggagg aaataaaaat gaattataaa aatagaaaac gagcttatta
    15841 tcaggaaaat tcctgtatgg ataactttat ttgcaaaaat tgtggttacc ttgtagttcc
    15901 tgaaggagct ggtacacaac atagaaatca ttgtccacat tgtttacata gtcttcatgt
    15961 agatatcatt cctggtgacc gtgctgcaga ttgtgatgct aatatggaac ctattggagt
    16021 gtgggtgaga aaaaatggtg agtgggcaat tattcatcgt tgtacacgct gtggacatct
    16081 tagttctaat cgtattgcag ctgatgataa ttcagtgaaa ttaatgtcaa ttgcattaaa
    16141 accactgtca cagccccctt tcccactaga aagaatcatt gaaaagttgg aaaaggagga
    16201 aacagaatga atggtatgag acaaatcaat ttacaagact atggcctaaa caggaatttt
    16261 attactcaaa gtatgggata tcatgattta taccttgctc gaattatctc gcaggaaaag
    16321 aatttttata aagctgtatg tgtaaatggt gtactgtttg cagaagtttc aggaaaacta
    16381 cgttttgagg ctactataca atctgatttt cccacagttg gtgatttcgt tatgttggat
    16441 cgttgcaata ataaaaatgg taatgctatt attcatcatg tgcttcatag aaaaagcttg
    16501 tttgtgcgta aggctgctgg aaaggtaaat gaggaacagg tggttgcagc taatattgac
    16561 attgtttttt tgtgcatgtc actgaataag gattttaaca ttcatagatt ggagaggtat
    16621 ctgtctttaa catgggaaag tggagctatc ccagtagtag tacttactaa atcagactta
    16681 tgtgacaatg tgcatcgtaa attacaagta gttaattcta tagccttggg tgtagatgta
    16741 gttgttacct catctataga gccagatggt tataaacaga tttctaaata tattaaacca
    16801 ggctgtactg ttgccttact tggttcttca ggagttggaa aatcttcatt aattaattgt
    16861 ctgctgggtg gagaattttt aaatacaaag gaaattcgca tagatgatga taaaggaaga
    16921 catgccacga ctagacgaga gctattttta atttcaggtg gtggtattgt tattgacaca
    16981 ccaggtatga gagagttggg aatgtgggat gtttcagaag gtttagataa aagttttgca
    17041 gatatagaag cttatttcgg aaaatgtcat tttcgtaatt gtactcacac ctcagaacca
    17101 ggatgtgcta tttgggaagc tattgctcat caagaacttt cgcctaaaag atgggaatcc
    17161 tatcgtaggc ttaaaactga agcagtattt gcagacaata aaagcgaata cttagcaaaa
    17221 aaagaaaaga aatttaaaaa tatctcaaaa ataaataaga agattagaag ataagtgtag
    17281 ttttttttca ttatatgagt ctacatacta ttaatatatt aaatttgcca tcaatttgtt
    17341 actttaatgt gattttgatg gcaaatttgg tttgtggtat tatttaaggc aatttattaa
    17401 tttttttaat aaagtttgat tttttatata atgatatata catatgataa cgtatacata
    17461 tttgttatga taggctcttt tataaagcgg tcttatgtaa gataagaaaa ggcatattta
    17521 tactaataca taatagagta tatgcttatg tatgatagag gtgtttctta aaagtaaaac
    17581 tgtaatatta ctaatttata aagtaaaaat acatgaatta taaaatataa ataagatatt
    17641 tataaaaatc attgttttaa tttataataa aaattttttg ttaaaaataa tgctcgaatc
    17701 tataaaacta ttaactaata tttgtaaatg tatacttttg atgatagaat ataattaagt
    17761 taagcttgtt ttttataaaa cataaattta ttttagagag gtaaaataaa tgaaaatatc
    17821 tagtcaatat agaagtcaat attcatttag acatgaaagt aatgtaaata atacaagaat
    17881 aaatgaaagt atagttaaga aaaatgaaat tgtaggaaaa gacacttatt tatctaatat
    17941 tatgaaacaa aagcaagaac ttaatgatag aattagagat ttaaaagata gacaagagat
    18001 ttatactaaa aaaataaatg aagcaattaa gaacttatat aaatcagaaa taagagaaac
    18061 aactaataac ttttctaata tagaaatagg tattaaaaat agaattatag aagataaaaa
    18121 taaaagtaca atgttagatg aaaattcaac ttatctaaat acaaatgatg aaaaagaatc
    18181 tttaatcgct aaagaattta atgaaaaagt tgaagaagaa atattaaatg atgaaaaatt
    18241 agaagagtta gaacaaaaaa ggattataaa gaggattcta ataaaaaaga gacggtatct
    18301 gaggacttat ccttagtagg taaaactcgt gaagagcttg aaaatatgct taaaaatttt
    18361 ataaatttaa cacaagaaga aataatgaaa cttgagtcga gaatagaaaa gttagataaa
    18421 gatgctgaag aatacaaaca aaattcaaag actaatatat ttgataaaac agatgaacaa
    18481 aaaaaacata taaatgtact ggtttaaaca atatgtttta aaaaaataag tttattatat
    18541 aaataagggt atagaaggtc taggatttta tgcggcagca ttagcgggtg gctcatatca
    18601 gagaataggc tttggaaaac ttaaccctat agaagtaata gccgatggag attggatttc
    18661 ttacaaacaa gctcaagata cacttacagt aatcaggaat tttttaaata gttttgactg
    18721 gagaaatgca tctgagatgg aaagagctaa tagagctgca aaattggtaa ccgaagcaaa
    18781 atatgcagac tctaaatatt gtaacatagt ctatggtaat ctgcttgata aacgtggtgt
    18841 atgtggaagt tttgcaagta gctttcattt attaacaagg ttaatgggaa tggattcact
    18901 atctatatta aatccatcat taaatcatgc ttggaactat atacaaattg atgggaaatg
    18961 gtataggttt gatgggtctg aaatatcagc ttttggtgga acattagact ttgattactg
    19021 taaattaaag gatgcaacga gagagatgac tacttactat gatgctaagg cattgtctat
    19081 attaggattt aatcaataaa agcaaaaaag gctctttata aagccttttt aagtgttttt
    19141 taagtgaaat tttttatatc acatcagttt taatcaaaaa atataacatt ttggacttac
    19201 ttttaaaaat aaaattatta aaatataata ttaatcatgt taataatgtt tttagtttat
    19261 tatataatag tggttatgtt gactattgta gttttattaa gaatagttgg tttatataaa
    19321 tatatgcaaa gataacttgt tgtttacttt atttattagt atatagtaaa agatactcta
    19381 ataaatttgc ttattttaat atcttttgca ctaaaaccaa ctaaagtttc ctttattatt
    19441 tcgttttgaa taatacaaag cataatccga ttgtttgtat aaagactgga aagtatcaga
    19501 gttattttct aatttagata tacctataga acaagaatta ctctctaggc ataattctaa
    19561 aactttcttt gctttatttt caacattctc ttttgattga ttactatatt caaaaacagc
    19621 aaattcatct ccaccaagtc ttccaataat agcatcattt acgaagactt tagtcaaaat
    19681 ctttgaaaaa tgtattagta tttcatctcc tcgaacatga ccaaagatat catttacctg
    19741 tttaaaatta tctaaatcaa agatgtaaaa gtatccatgt gaaacagttt taagcatttc
    19801 ttttatgtgt acttcaaaaa aatgtctatt gtatagtcca gtcataaggt ctttttctga
    19861 ttttagttta aattcatttg ccattgctag tgcttcttgt gctttttcag ttattgtttt
    19921 agggtaatac tcatctttca tttggtcaaa gtttggaata gaatggtgaa catggataag
    19981 tttaatcata tcatctgtaa ttctgtatac catagtaaat cgagtatcca tttctacata
    20041 gatatctttt tctaggtaat ctttttctat tacccaaaga gtaccatata ctataaaagc
    20101 ttcgtctgta actttggtac atgaatacca ctcatctata atctcaaagt ctatttgctc
    20161 tgattctgtt tggtcttttt caagacctat aataaattct tctaatgttt cgtaaaattc
    20221 atgttttcct gttccaatta atgcaaggtt gtcatctaat aaggaaataa taaatttaag
    20281 gttttcttct tctggattta aaatataaat atgccaaatt ttttttgata aatcccttaa
    20341 ttgtttttgt attgtattat tttccataga ttaccccctt aagatttaca gtttatattt
    20401 taatgtatta taagttgaac tcttattaca atattattat aatatagaac taaagaaatt
    20461 acatcctgta taggtaaaag tatatctttt acaataaaaa tattacagta aaataactcg
    20521 ttttaatata catattatat ttttaattta taaatgaata ttataaatga aatttaaatt
    20581 caaccctgaa gaaataagct aaatgtatta gtatataaat ccatacttga acataaataa
    20641 cctttatgat atgatatcaa taataatatt atgagaggag tgccttgatg tcggagttat
    20701 ataatggtaa ccaacaatta aatagttgga gacacatata ttctgtataa tcagaaattt
    20761 taagtgtatt tttaatacat ttaatttttg tgtcttattc tgcctttata gataactgac
    20821 atatgacaac aaatagtatt taaaatatat attactttgt taaattatgg tgtagtaggc
    20881 tttaatctgc tacactttta ttaattttgt atagtattat tttgataact attttgaaat
    20941 agagagcatt aatttttcta gtccacagga gttttctgtt tttgtgctgt taaatataag
    21001 cacagaaaaa ggcagatata tacctgtgga ctttttttat ataaattatg aaatatggag
    21061 gtagttaaaa tgagtataat tgaacttaat gaactgacta aatcttatag aagatttgag
    21121 aaaaaggcag gatttatggg gagtgtaaag tctttattta ataggaaata tacagaaaaa
    21181 acagctatat cgtcatttga tttaaagata gaagaaggag aatttgttgg attaataggt
    21241 caaaatggag ctggcaaaac tactcttatt aagatgttaa ctggtattat tgcaccgtct
    21301 tctggagaaa tatctgtaat gggttattac cctaacaaat tagaaaataa ttttaaaaag
    21361 caatatgctg ttgttatggg ccaaaaaagt caactattct ttgaattgac accagcagat
    21421 acgtttcttt tatttaaaga attatatgat atatctaatg aagaatataa aaaaaacttg
    21481 gattacttta tcgaattatt tgaggtatct gactatttaa atgttcaagt taggacgctt
    21541 tctttaggag aaagaatgaa aatggaacta atagtagccc ttctacataa tccaaaaata
    21601 ttatttttgg atgaaccaac tataggctta gatgctatag cccaaaaaca aattaggtat
    21661 ttcctaaaag aagtaaatga aacaaaagga actactatta tacttacttc tcattacatg
    21721 gaagatatta agagtctctg tagtagaagt attgttgtag aaaatagccg taaaatatat
    21781 gatggttcta cagagaaact atttaatcat tttcaaaaat ataaaaaaat aacagttagt
    21841 ttttccaatg aaactaacat tgtatttcca aaagattgta aagttgttga acaaacaaat
    21901 cataagatat cagtaattgt gccaaaagaa agaggaaatg aaacgattaa atggattttt
    21961 gataaatatg atgttaaaga tgtaatgatt gaagaagaag atataggtag tattgtagag
    22021 aggatatata agaaaggaag tgatgagtat tatgagcaac tataatgaag caagttcaaa
    22081 tgattcaatg ttttataaat ttaaacagag tattagaagg tattatgaag tagccaaaat
    22141 taactttaaa gtgcaactta tctggagatt tgatgtggtg gttaatatgg tgtttacgat
    22201 tgtaaaaatt ttatttgctt atattttatg gaaagcaatt tttggtgaaa ataaaatagt
    22261 ctctggtttt acatttaact ctatgttgtc ttactatata ataagctcat ttttagcagg
    22321 tattgatatg tcaaagagta ccagtgaaga gatatgtaat cgtgtaaaat tgggcacctt
    22381 ttcaaagtac atgattattc caattaatgt tcaaaattat tttattgcta aaaattttgg
    22441 aatgacatgt ttttattttg tatttaatat gatttctgca tttgtatgga tttttatatt
    22501 taaaatagat tttgccttta catcaaatcc agttcaaata ttaatttcaa ttttgatgat
    22561 ttttctaggg tttattttta tgatgaaact ccacttcttt attgggattt tgtcatttaa
    22621 atttcttgag attagttttt ttagaatgat aattgaaaat attgttgaat ttattacagg
    22681 aacaataata ccacttgtac tcttaccaga aactatagta acagttatga aggtatttcc
    22741 attttattat acaacatatt tgccatctat gttactaata ggtagaaaca gcaatgaggc
    22801 aatacaagga ttaattattc tagcattatg ggttttattt ttttctgtag taaataaagt
    22861 tatgtataaa agaatgagaa cattatttga tggggtgggt atatgattaa aaaaaatttg
    22921 aaaataatat ttatgttatt aaaaatgaaa ttatcaaaga tgatggtatt tagatttgat
    22981 ttttttggag cattttttgt agatagttca ttatttatat tacaacttct aatgtttaat
    23041 agtatatatt ctcatgtaga ctcaataggg ggatggcaac aaggagagat gttaatattt
    23101 ataggaactt tctcattaat aaatgcgata aatatgacga ttttcttttt tggtatttat
    23161 gatattccac gaaaaatcca ggaaggagaa ttagactact atattacaaa accattagac
    23221 ccactattta ggttaacatt tgaaaatatt aatattggtt catcaccatt aattctagca
    23281 agtatactta ttgttttata tggaatatct gagttaaact cagagataac tttagttatg
    23341 ataataatat atattttcat ggtgttattg atgacattac ttttttatga tgtatgtatc
    23401 atacttagaa cagtttcatt ttttgttata tcatcttctg cgataatgag attagaagat
    23461 aatttattgc caatgaacat gaaaattcct ggtgtaatat atgaaggtgt ttttaaagta
    23521 ttattctatc taatcttacc atatggaatt atgtcgacaa ttccgactca aattttggct
    23581 gggacaataa caattaatgg attaatatac tcaattttaa ttgtattttt gtttacattg
    23641 tttatgtgga gattttggaa gttaggtatg agacattata aaagtgcaag tagttaataa
    23701 attggtaaaa atagaaaatg tataattata tataaatacg tttcatatga cactaaacta
    23761 tgtaaacaag tatttgcact aaaggcactg aaagctataa aatgattaaa gtaaggattt
    23821 aaaagttact ttagtagaaa tataattgtg taaaaaagca agattaatta aaactatatc
    23881 cgaataatta gatataattt ttatgcagaa aggaggtctg taaatgtcta attgtttgga
    23941 atatatttta aaaataatta atgtacagaa aaataaacaa aatgagtgat gtatattatt
    24001 cagaaaatca tgaacaagat aatctttatg agcaagataa tttactggca caaagattta
    24061 atttatttac tctattgaag tttgcatttc ctactatttt tatgatgatt tttatggggt
    24121 tgtatacaat cgtcgatact atatttgtat caagatttat taatacaaat gctctatcag
    24181 caattaatat tgtatgtcca attattaatc ttatagttgg aattggaact atgcttgcca
    24241 ctggaggaag tgctattgta gcacgtaaga tgggaaatgg tgatattgag ggagcaaata
    24301 aagctttctc tttaatcgta ttatcaggag ttgtaattgg agtaataatt actggcatag
    24361 gtattgtatt tatggattat atcattatat ggttaggagc aagtgaagtt ttaattaaat
    24421 attgtcgttc atatcttttt attatcttgg tttttgcttc tgcgaatata ctacaagtat
    24481 tatttcaatg tttatttgta acagctggaa agcctcaatt tggattatgg ctagtgttag
    24541 gtgctgggtg tgctaatata attcttgact atgtgttcat tgttctgtta caaatgggta
    24601 ttagtggagc agcacttgca actggagtag gatacctaat tcctaccatt tcaggtatgg
    24661 ttttcttttc aaaaaataaa gggttactta aattttgtat acccagacta gataaaagaa
    24721 tattatttga agcttgtttt aatggttcat ctgaaatggt gggtcaacta tcaacagcag
    24781 ttactacttt tttatttaat gctatgatga tgaaattaat tggtgaagat ggagttgcag
    24841 ctattacaat aattatttat tctcaatttc ttttaacttc gctttatata ggtttctcaa
    24901 tgggtgttgc accaataata agttataact atggaagtga aaacaatacg cagttaaatc
    24961 atgtttttaa gatttgtata ttatttattt cagtagcatc tttattggta tttatctttt
    25021 ctatatcatg ttcaccaatc attgtagaga ttttttctca aagaggaagc cgagtttatg
    25081 aaattgcaag gtctggattt tatatatttt cttttagttt tattttttgt gggtataata
    25141 tttttacttc gtctatgttt acagcattat caaatggaaa ggtgtctgca atattatctt
    25201 ttctacgtac atttggattt ataactatag gtttattgat attgccaaat atattaaata
    25261 taaatgggat atggttagca gtaccaagtg ctgagatttt aactttattt ttaaccgttt
    25321 tttgtatatg taagtataag aatagatata aatatatgta gatattaaat aaatagttta
    25381 ggtagatatt ttatgtttta ataagtaatt caattagtgg tagatagata ctattgaaaa
    25441 ttaaagttat aattgatata aagaattaat aaaaataaca tataaaagtt taaaagggga
    25501 gttgaactta aagtttgatt agaaaaagtc aattttaaga caactccttt tattttacta
    25561 ttgtattaat ctgtatctgt aagtgtatta gtctaatatt ataaactttg gatgaaatta
    25621 attttctata ggttttaatg tatcatacac tttattttaa agaaactcat cttaattttc
    25681 ataatgattg aatttgtata ttagagatgt tactattagt aatgtaaaag caagagtagt
    25741 gaatataaaa aaacaagata gtgatactgc aagattagaa tataataaat ttttatggag
    25801 agatttcaat atggatacat attttaataa aaaaatatta cttgttgatg atgaaaaaga
    25861 tatagtggat ttaattgaag aagtacttac aaatgatggt tttaaaaaca ttattaaagc
    25921 ttataatggt ttagatgcta tttcgctgtg taagataaca tgtccagatg ttgtcattct
    25981 tgatataatg ttaccagata tagatggtat agaagtgtgt aaaaaaattc gagagttttc
    26041 atactgttct attctttttc tctcatcaaa aaatgatgat attgacaaaa tactaggtct
    26101 tagtagtggg ggagatgact atataacaaa gccattcagc ccaagggaaa ttgtttttcg
    26161 agttaaagca caattacgta gacaacagta tcaaagtatt gtaccatcag atagtgaagt
    26221 tattaaggta ggagatatta ctattgatat agaaggaaac cgtgtatata aagatagaaa
    26281 tgagattgag ttaactggaa gggagtatca tcttttaagc tacatggctc aaaatgtaaa
    26341 taaaattata ggaaaagaaa gactctatga acaagtatgg ggagtatata gtagtatttg
    26401 tgacaataca attatggtgc atatacgaca tataagagaa aaaattgaag ataacccctc
    26461 aaatcctaaa atacttataa cggttaaagg actaggatat aaacttgtga atagaatcga
    26521 ttagaataga ttttaatatc aattaagatg tctcaaattg aattttttag ttaatgaggc
    26581 attcttttat gtgtgaaatc aaatgtattt tcgttatctc aataatgaag aagaggtcta
    26641 tctctatttt gagatagcct cattaagttt atatcctggt aatgtacttt atgttctaat
    26701 aaatatttat ttttttagtt tatttgcagt gaaacattaa ctagaacatt aaaatttgca
    26761 ttgaaatacc aattaaataa tattgtatat tgtgtataaa cttatactaa ttgtttttct
    26821 atattaaata ctttatcaat ccaaccttta taaaattttt cttcatggga aactaggatt
    26881 atacttcctc taaactgttt caaagcattt tgtaaagcat cctttgtctc tgcatcaaag
    26941 tgattggttg gttcatctag aataagaaaa ttacaaggag ataacatcat acaacataat
    27001 ttaaccttag attgttcacc accacttaaa gtagatacac ttcttgaaac atgctcttcc
    27061 ttcacaccac atctggctag atgacgtcta atctcttttg tatttaattt tggatattta
    27121 tctgctacta tttgaagtgg ggttttatca ggattttccc attttaaatc ttgctcataa
    27181 taaccaattt taacttgttc tgaaaactgg aaatctccag atatacgagg aatatctttg
    27241 actaatgtct ttaataaggt agatttaccg attccattaa aaccagttat aactattttt
    27301 tgacctccat caacagaaaa atttaatttt ggaagtaaag aatagtagta tccaacttca
    27361 agatttgtta tagtaagagc attttgagcc gatatctcaa tttctgaaaa ttgaatattt
    27421 ggttttcctg taaaactagg aggagctatt ctttctattt tatctaattg tttttgtctt
    27481 cctcttgcaa tttttgaatt tactccagct ttatttttac gtataaatat ctcttctttt
    27541 tctattttct tttgttgtgc ttgatatctt ctaatataat cttctcttaa atgtgatttt
    27601 tgtttaagga actctgaata ttttccatga tatttcttaa tcatgccaaa ttctatatca
    27661 agaatacctg tagaaatttt ctctaaaaag tcaaaatcgt gggaaactac aataaatgct
    27721 ccattaaatg tatttaaata atctgagagc cattcaacat gttctttatc taaaaagttt
    27781 gttggttcgt ctaataataa aacatcatgg ttagaaagta gtaattttgc taaaattacc
    27841 tttgcccttt gcccaccact aagctctcca acaacacgat tcattcctat tgaatctaat
    27901 ccaagaccat ttgctatttt atttatttca ttatctatat aataaaaatt atttgctatt
    27961 agttgttcct gataatctga tgctttgatt agatgttgct catttccaga tatagcactt
    28021 ttttgataga gtaaattcat ttccttttct attttataaa gtttttcaaa tgcagtgtgt
    28081 aaatagagtg atattgttgt atctttattg atttctgcat attggtctaa atgtcctata
    28141 ttgatattag gttgccactt gatttctcct gaatctggaa caacttctcc caataaaatt
    28201 ttaattaatg tacttttgcc tgcaccattt tgaccaacta cacctatatg ttcacctttg
    28261 aataaatcaa agttagcttt ctcatacagt gatttatcca taaaacattg tgataaatta
    28321 gttactttta ataaactcat aattcaaact ccttttcttt tattaaaaaa tttatggaaa
    28381 taaaaaagca gaggaatggc attgtaaaat gccgacctct gcttttggaa aaacaaaagg
    28441 tcatagacaa gcattgttgt ctatgaccag aaaatctata ttatctatcg gcaaataggt
    28501 aattttaaat aagcatagcc aaaaaactat actaaaccta tatttgccga tgaagataga
    28561 aagctttaag ctttcagatt aacctctaca caacgcttca tcgtatacat ttgtgtagag
    28621 ttaagttttc taacaagttt aactaattct atataaatca taattattca catcctaatc
    28681 ttagtattaa ttctagttta tctatattta aattgtactt ttgtactttg tggattatct
    28741 tatttattta aaatatgttt tattaattat agcttaagtt atgttatcat aatacttagt
    28801 tatagtcaat aagaaagtta tatttactat atattatatt aatttagcaa atctaccatt
    28861 acaaagttat ttgaagattc gctaccaata aagttttata gtctgtaaga aaattaagat
    28921 tatgagtttt ataattttga tatagggata ctattttcat aaatattata atatgtagaa
    28981 gtatatgtct agtattgttg aattttcaat gatatattaa ggttctaata tattatacaa
    29041 aataaaaaca ttaattcaac ttaagtaatt ttaagtatca tatgtgtttt gattaaatag
    29101 acctaaaatt ttaagggtaa ataacatatt aacattaaat tttattgaaa tgattaaaat
    29161 tatttactta atgtaaatta ttagctaaaa ttgtactaaa taattaagga ggtgagtagc
    29221 tttggatgag atagatttaa aaataattaa ttctctaaaa aagaatagta gggcttcaac
    29281 ctctgaaata agtagacaaa ttaatttatc agtaccagca gtctcagagc gtataaggaa
    29341 aattgagcag tcaaatttaa ttcaaaagta cactattcaa attaatcggg aaaaaacaaa
    29401 atataagctt ttggctttta tatttatcaa tactgattta tctaaaagta tgaaagaatt
    29461 taagaaaact atat
//
LOCUS       DAEORZ010000039        28597 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEORZ010000039 DAEORZ010000000
VERSION     DAEORZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.039"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            245..1096
                     /locus_tag="KRH39_001564"
     CDS             245..1096
                     /locus_tag="KRH39_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG8511478.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            1100..1570
                     /locus_tag="KRH39_001565"
     CDS             1100..1570
                     /locus_tag="KRH39_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511479.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            1583..2893
                     /locus_tag="KRH39_001566"
     CDS             1583..2893
                     /locus_tag="KRH39_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8511480.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            3105..3851
                     /locus_tag="KRH39_001567"
     CDS             3105..3851
                     /locus_tag="KRH39_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8511481.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            4213..5754
                     /locus_tag="KRH39_001568"
     CDS             4213..5754
                     /locus_tag="KRH39_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG8511482.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            5789..6895
                     /locus_tag="KRH39_001569"
     CDS             5789..6895
                     /locus_tag="KRH39_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG8511483.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFQGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(7509..8795)
                     /locus_tag="KRH39_001570"
     CDS             complement(7509..8795)
                     /locus_tag="KRH39_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG8511484.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(8918..12016)
                     /locus_tag="KRH39_001571"
     CDS             complement(8918..12016)
                     /locus_tag="KRH39_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG8511485.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKYARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKYLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            12741..12899
                     /locus_tag="KRH39_001572"
     CDS             12741..12899
                     /locus_tag="KRH39_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511486.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(13186..13890)
                     /locus_tag="KRH39_001573"
     CDS             complement(13186..13890)
                     /locus_tag="KRH39_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8511487.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(13881..15488)
                     /locus_tag="KRH39_001574"
     CDS             complement(13881..15488)
                     /locus_tag="KRH39_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBG8511488.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(15742..16920)
                     /locus_tag="KRH39_001575"
     CDS             complement(15742..16920)
                     /locus_tag="KRH39_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511489.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(17146..17640)
                     /locus_tag="KRH39_001576"
     CDS             complement(17146..17640)
                     /locus_tag="KRH39_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG8511490.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(17641..18402)
                     /locus_tag="KRH39_001577"
     CDS             complement(17641..18402)
                     /locus_tag="KRH39_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG8511491.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            19124..21958
                     /locus_tag="KRH39_001578"
     CDS             19124..21958
                     /locus_tag="KRH39_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8511492.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFSETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYSSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            22001..23245
                     /locus_tag="KRH39_001579"
     CDS             22001..23245
                     /locus_tag="KRH39_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG8511493.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(23514..24188)
                     /locus_tag="KRH39_001580"
     CDS             complement(23514..24188)
                     /locus_tag="KRH39_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8511494.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLEIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDNKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMGLINKLGIENQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKKADRIITIKDGK
                     IDEKNY"
     gene            complement(24423..26972)
                     /locus_tag="KRH39_001581"
     CDS             complement(24423..26972)
                     /locus_tag="KRH39_001581"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8511495.1"
                     /translation="MINIKLAMSYLKKQKGKTLSLILSIGIAVILVFSLSVIKESQGQ
                     SDINHAYKNSGDYHAFYEDVDSKIVKKLNSEEDIKEFNNVLKFGEIVSKDNGSSINLC
                     SYNKNFLDSTRYKLIEGREPRNSNELVIEQKVLDKMKLDVKLNQTIDFQIVNEYVNKN
                     NENSIYSKDKQFKIVGILSKTDEYYEGLNKYKVRAFTYSKDGKDIVPYELITHMGTLK
                     LNTKDLDTQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQIFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELIINTTAIIESIIMSIIAVILASVVPVYKAGNLSIIDAIKKTDK
                     LNYIPKFLLLKSHKKVIGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIDDVTDIESNI
                     YLKGYLRNADLEKGYLDELKRTEINNTNEPTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TKGDYKNILLVNNFYSRIELSNNTQIIKSPKLGDLIDIKTPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQVIFNEKDLKELTGMNYYNKAFVKIKKGTDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAVGMSKKNVKRMLIKENIIYGVLSMLVAGIISSFSYYKMVSETNKLNKQI
                     YGISNSIKFEIPIYEILIFGLISVFVCIIAVYFSNKMINKLSIVSGIKENE"
     gene            complement(27513..>28597)
                     /locus_tag="KRH39_001582"
     CDS             complement(27513..>28597)
                     /locus_tag="KRH39_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8511496.1"
                     /translation="YIVDKWKSMFISLLYWMILIGFDIIGLSIVGTLNSIQNMTTLLD
                     NNLLKLELIMISKSLLIALIPLLKVIRLKIKINKKDCIYSSIPIISNIIGVIVIFEFI
                     FKDKNIDSTENLIILIISIVFLLSNISLISIIGRIIKDNNLRIEHEITKEKMNMQYKY
                     YLNLQEYQLKIRKLYHDMSNHVICIQNIYGKNEFADKYIKDINKQIKECNAMFNTQSM
                     ILDVILSEKKSICDMNNIDFLVDINFLECNFIEMPDVCSIFSNIIDNAIEACNKIKDT
                     SILKKIKLRGTVINRLFVIKCENTKINEVVLKNNKLITSKKDSFLHGIGISSVKGSVE
                     KYNGNVEVYSDKNKFTITIYIPLARN"
BASE COUNT         9967 a         4438 c         3515 g        10677 t
ORIGIN      
        1 tatttaaaca attttttcat aataaagcta tattacaata tattcaaaat atgtaatatt
       61 ttagatattt ttcgttgaca atgttaaatt tttgttttat ccttaattaa ggacatgtta
      121 ttgacatttc tgaaacttat ttgaaatagt tttttcattt tatttaggaa aacgtattat
      181 acttaagaaa acgtattttc tcatttcaga taagtaaaat atttattctt ttggggagga
      241 aatcatggaa attaataaaa aacattttat tcaagtattt ttaacttgct taatcttggt
      301 agtaatatgt gaatttatag ggcaaagaac tatagctatt ggaaaagcct ctatagtact
      361 gttccctatg ctttatgcag ttataattgg acttataata acaccagatt tattaggtaa
      421 aaaaattaaa gcgcttaaaa aagtggttgg agaaaaagaa ataaatattg ctggtgaagt
      481 tgttggaatt gcactagtaa tgcttggtat aaaatatggt actacagttg gaccaaacat
      541 tgacaaaata attcaagctg gaccagcatt tatcgctcaa gaatttggtc atatacttgc
      601 accaatcgtt gcattacctc ttgcattaat gtttggaatg aaaagagaag ctgttggagc
      661 tactgcaagt ataagtcgtg aaccttcttt aggagttata tcagaaaaat atgggataaa
      721 ctcaccagaa ggtagtggtg tacttggtac atatttaatg ggaacagttc ttggtactat
      781 atactttagt gtactaggtt caatatctat ttatagtggt cttcacccat atgctttggg
      841 aatggcctgt ggtgttggta gtggaagtat gatgacagca gctgctggtt cattatctac
      901 catggtacca cctgaaatgg cagatacaat acttgcctat gctgcaacaa gcaatatgat
      961 gtctagtata acaggaatta tattcttagt ttttgtatcc ttaccattta ctaattttat
     1021 gtataagatt ttagaaccaa aattttcgag aaataaaaaa actaaagaaa ttaaatctac
     1081 agaaggagag tgctaataaa tgtttgctaa tttaaattta aagaaaagtt catatcaatt
     1141 attggttatg tctgttattg gagttataat tcttatagga caaagaatta gcgttggtac
     1201 atctatagtt acagctcttc ctggtatggt gatgctaata ttagctgcta tggcagctat
     1261 gattataaaa gatttatttc ctaaatcaat ctttccagct tttggatttg caactataat
     1321 cggcctttta cttagtatgc catatagtcc aacatctgaa gtattcttaa ctaacactaa
     1381 taatattaac tttatggcta taactactcc actactagct tttgcaggaa tttctgttgg
     1441 aaacaaaata gaagctttaa aagaaatgtc atggaaaata gttataattt cacttattgt
     1501 atttacaact atattctttg catgtgcgag tatagctcat atagttttaa gtattcaagg
     1561 taaaatatag gaggaattta ttatgaataa agaacaatta aaatctctag taataaaaac
     1621 tatagatgaa aatagagaaa aaatattaaa aataggtcaa ggaatataca aaaatccaga
     1681 atatggatat aaagaattta aaactacaga agctgtttca aacttttttg aacaagaatt
     1741 aaatttatct gtagaaaaaa atatagcagt tacaggatgc aaagcagatg caaattctag
     1801 caagaaaggt cctcatatat ctatacttgg agaattggat ggtatatcat gtaaagaaca
     1861 taaagatgct aatgaaatag gtgcttctca tacttgtgga cataatattc aaatagctgg
     1921 aatgttaggt gctgctgttg gtttagtaaa aagtggcgtt ttagaacatt tagatggtaa
     1981 agtatctttt atggctactc ctgctgaaga gtttattgag ttggaataca gagagcaatt
     2041 aaaaaaagaa ggaaaaataa cttattttgg tggaaaacaa gaattaataa aaagaggtta
     2101 ttttgatgat atagacatat ctatgatgtt tcatgcttct gatttaggag aagataaggc
     2161 tctagttggt cctgagagta atggatttgt tggaaaaaag gttaaattta taggtaaaga
     2221 atctcatgct ggttctgctc cacatgatgg aataaatgct ttaaatgcag caatgcttgc
     2281 tataaacaac gtgaatgcac ttagagaaac ttttaaagag tctgaaagag ttagattcca
     2341 ccctatcatc acaaaaggtg gagatatagt aaatgttgtt cctgctgatg ttcatatgga
     2401 atcatatgtt agagctagaa ctataaatgg tatgattgat gctaatgaaa gaataaataa
     2461 ggctttgatg gctggtggaa tggcagttgg agctgatgtt gagataactg aaattcctgg
     2521 atatcttcca atattacgat acaaaagtct tgataattta tttaaaaaca atcttgaaga
     2581 tttaggtcta aaaggaaaag ttattgatgg tggagatttt acaggctctt ttgattttgg
     2641 agatgtgtct catataatgc ctactcttca ccctatgatt ggtggtgtaa aaggagctct
     2701 tcatacaaga gattttgaaa tagttgatga agatttagct tacataattc ctgctaaagc
     2761 aatggcactt acagttgtag accttctttt tgataatgct aaggatgcta aagatatatt
     2821 agataccttt actccagtta tgactaaaga agaatatcta acattcttag aaaaacacga
     2881 taaaacttta taagtctatt tttataatag ggtggtatct actgtaaaag ttataccact
     2941 ctttgttttt gtaacagtat acttcttttc ttttagtttt tagattaaac taaaatatat
     3001 tatcctcctt aaaactttta gagatacgtt gaaatatcag tagaactatg ctatattctt
     3061 tctaattaat ataaatttat attctaattc aaggtggtga aaaaatgttt aatgcaggaa
     3121 atcgaattat aaatacatgg ttgtacccta ttcaagatgg ttttgtcttg attgatactg
     3181 gatatgaaaa tggttttaaa catttcaaaa aacgaatttc taaattcaac ataaaaatca
     3241 aagatattcg atacatattt ctaactcatg ctcatgatga ccatgttggt tttctaaatc
     3301 agattctaga cgaatctcct aatacaaaga ttgttatgca tgataaagct ttagaaattc
     3361 tatataaagg tcaaaactct tttattggtg gttgtaccag ccgtactgcc tttctctttt
     3421 gccaaattat gaaactattt ggaaaaggtg agcatagttt ttctccactt aaacaagaat
     3481 ttgaaaaaag gtgtattctt gtgtctgaca agaatcgtaa agaaattgaa aaagaactaa
     3541 atggaaaaat tatagataca ccaggacata ctgctgattc aatttcacta ctaattgatg
     3601 atggtgtttt attttgtgga gattctgcaa tgaatggatt tccaagtact cacaaaatta
     3661 ccatatttgc agaaagcaaa ctagaattta ttcaatcttg gaaaacaatc atcaatacaa
     3721 aacctaaaat gatttatcct ggacatggaa aaccatttga atacagtaaa ttagaacaaa
     3781 atctaatcta tgtaaataaa ataaatttat atcctctaca taattcaaaa gatattcaca
     3841 ggtttttgta gaaaatacaa agtaaattta tttgattaat taaaaaatct aacactttac
     3901 tacgctactt attttaataa cttaatcttc cactagcttt tcataaaatt ttgttttaaa
     3961 attaatttta aagcgcatat gttatttaaa tacattagat ataattcgtc attaatctac
     4021 ttaaagcata tttaactaac accactcaac cagaaaattc agaaaactgc attgacacag
     4081 gaaaaagttt attttatact ttttttagga catattgtgg acacgtctta aatcgacaag
     4141 cagaatttaa attgtaagat taaccacaat acttattttt taattatatt tttaaacatt
     4201 agggaggata ttatgaatga taacgtagtt aagaagaaaa acttttttga caggacatta
     4261 aacagaatag aaactgttgg taataaacta ccagacccag ttacaatatt tttagggtta
     4321 tgtgtacttt tacttatact atctagtcta gttggttcta tggggatatc agtagttcat
     4381 cctggtactg gagaaactat aaccgctgta aatttattga ctgtagaaca gcttcaaatt
     4441 cttttaggaa acatagtcag caatttccaa gggtttgctc ctttgggtct agttctagta
     4501 actatgatag gtgctggtgt atgtgacaaa acaggactta tgacagcaac aatcaaagca
     4561 tcagtaagca agattcctga aacaagagta actcttgttg taatgactat aggtatgctt
     4621 gccaatatag cttcagatgc aggtacaata ttatttccac cattagctgc acttgtatat
     4681 cttggtgttg gtagacatcc acttataggt ttattttctg gatatgcagc agtctgtcta
     4741 ggatttgccg caaacattat gataagtgtc aatgatatac ttgctgcaag ttttacagtt
     4801 ccagcagctc aaatgattga ttctggattt caagccaatg caacaatgaa cttatttttt
     4861 atgatagcat caacttttgt tctaatactt ttggcaacat gggtaacaga aaaaattgta
     4921 gctcctagat ttggtaaata tgaaggaaat gcagaactag atgttgatgg aagtcttagt
     4981 gatgtagaaa agaaagggct taaaaaagct ggtataagta tattaatata tgtggctatt
     5041 atagtagctc ttagtgtaat cggtaaaaaa ccattcttag ctgacccaga aacaggagat
     5101 ttactatcaa acaatgctcc tcttatgaaa ggtatggtac ctataatagc actagcattt
     5161 ttcatacctg gtgttgtata cggaaaaact ataggtaaaa taaagaagga taaggatgta
     5221 gtctctataa tggctagtgc aatgagtgat atgggtggat atataatact agcatttgct
     5281 gcttcacaat ttttgcaatt attcacaaat agtaatttag gtctgatact agctgtaaag
     5341 ggcggagaat ttttaaaatc agcaggaata aatggacctt tattgttgat aggatttatt
     5401 atattatcat gttttataaa tttatttata ggtagtgcat ctgcaaagtg ggctattctt
     5461 gcacctatat ttgtaccaat gtttatgatg gttggataca atccagcact tactcagatg
     5521 gcttatcgta taggtgattg tataacaaat ccacttagcc cattatttcc gtacttccct
     5581 attatactag cattcacaag aaaatacgat aaagatgctg gtatgggtac tgtaattgct
     5641 aatatgatac catactctat atcattctta atagtgtgga ctatactatt aatattgttt
     5701 atggtattta atattccact aggacctgga atacttcctt catatagcat gtaatttatt
     5761 taaatctttc taaaaggaga aataatttat gttagataaa aatagactta taaataattt
     5821 tatggatatg gttagaattg atagcccatc taatcaagaa ttagaaatgt ctaagtggtt
     5881 ggtaaactac ttaaaagaaa gaaatataga cgccattata gatgatgcag gtgaaaaata
     5941 tggtggtaat acaggtaatg tcattgccta tataaaaggt gaagaaggaa gtagaccact
     6001 ttgtttatgt gcacacatgg accaagttca accttgttta ggtgtaaaac caatactaga
     6061 tggtaatgta gttagaagtg atggaactac tacacttggt gcagatgata aagctggaat
     6121 agctgctata cttgaagctt tagaacatgt tattacagaa aaaataccac atagagatat
     6181 ttatctttgt tttacaatct gtgaggaagc cggtatgcat ggtgtcaaaa actttaatcc
     6241 tgacaacttg ccttgtaaag atatggtgat tttagattct ggtggagcta ttggttcgat
     6301 agcatacaaa gctcctgctc aacaatctat aaaaatctct tttcaaggaa agaaagcaca
     6361 tgctggtata gaacctgaaa aagggttaaa tgctatatta gtagcctctc atgcaataag
     6421 caacatgcac ataggcagaa tagattcatt gactacttca aatataggta aaatagaagg
     6481 tggtggagct acaaacatag taactgataa ggttacattg actgctgaaa ttaggtctca
     6541 tatacctgaa actttagagt atgagcttaa tcatatggaa aaatgttgta aggacgctgc
     6601 atctaaattt aatactactt atacttttga acataatatg tcttacccta gttttgaact
     6661 tagtagagat agtcatgttt tcaagttaag tgaagaagca ataagacaag ttggtgttgt
     6721 tccaaatcca atggtaattg gtggtggaag tgacgcaaat atattagcta atcttggata
     6781 taattgtgct atattaagtc taggtatgta tgatgtacac acagttaatg aatatgtaaa
     6841 tatagacgaa ttatatgata ctgccaaaat agtttatcat atgattaaat tataaatatc
     6901 ataatagaac tctttaaatt ataaaaataa aaacctatgt atcttcaact ttataagtta
     6961 tatattagag ttgttttaca taggttttag tattttatat aataatttgt tttaacattt
     7021 ttcataataa gctttgatat tttatatagt aatttgttct aacatttttc ataataagct
     7081 tcaatatttt gcataataat ttattctagc atttttaata ataggctcta gtattttata
     7141 ttaataattt attctagtat ttttaataac aagctctagt attttacata atcatttgtt
     7201 ctagcatttt atattaataa tttattctag catttttaat aacaagcttt agtattttta
     7261 taataatttg ttctagcatt tttaataaca agctctagta ttttacataa tcatttgttc
     7321 tagcattttt aataacaagc tctagtattt tatattaata atttattcta gtatttttaa
     7381 taacaagctc tagtatttta tataataatt tgttctaatt tttttcataa taagctttaa
     7441 tactttatat agtaatttgt tgtagaattt tatacaatat aatgatttat acagttttat
     7501 aaaatatctt aaaacagtat ttcagtaata ttttttggtc ttcctccacc aatactagtc
     7561 tcttttgcac atattcgacc taaacctgca tttactattt tatttaatat acgtcttgca
     7621 cttctttcac ttacatttag atactctgct aactcttttg aatcataaat cttacttttt
     7681 ctattctcat ttatagcaat aatttttgca actgactcac aactcattcc agtcttttta
     7741 gatatttcga ttaccttttc atcagatagc atcaaagaat aatttaattc attttcggaa
     7801 gctaaaggtc ctcttatatt ttcatcttca tctaccaaat atataccaat ttcctttgaa
     7861 tctatggagt gtcttaatgc tttatgtgca ttattttcag cctgatacgc agttactcca
     7921 gtgccaattc ctatactaag agaaaagccc atgtctttta tctctctctt tagattgaat
     7981 agcttatcat aattatactc attatcagct acccctttat gaacaaatac aacataatca
     8041 tttcttccaa gtaaaaataa ggaaccttga atacttctta catagtttac tacaagctta
     8101 tccatatctg atttttttat catgtcagaa taatagttat ctatcttctc tttataatct
     8161 attaaactca atatttcaac agcaatttga gaaaatcttg ccttacttag agcatattca
     8221 ctctttacct tgtcataact ttctttgatt aattgtatag tagcttgtaa acgaaataca
     8281 ggatagcctc tttcttttaa ttcattatat actgcaccaa aaccagttaa cattatatcc
     8341 gtcttatttt cttcaaataa ttttatatgc cattctacaa gttcagactc tcctacttcc
     8401 aaagaaaatg gattacaaaa tacactcgtt gctttaatat caaattcttt aagtgcatct
     8461 tccacaacat atctatcaac tacatctata ctgaatttat ctaattttat atttgcactt
     8521 tttattgccc aaaatgcctt cattatactc gtcccacttc tcggtacaaa tgaatgtggc
     8581 ttacttatct catacttaga ttttataaat tcataaactg cacatcctgt aaatataata
     8641 gcatcacatt cgtcttcaca tttagaaata acttctattg tatctactac tttttctctt
     8701 acatatattt tagtctttaa atcactgtct atctgtttta aatcatcctt tatcttaaac
     8761 ccagagtctg aaggacctat aatacccact atcataaact tcgctcctaa tccataaata
     8821 ttatactttt taaaaattat atcctcacta ttttctactt ctatttaatt attttgaact
     8881 actccatcct acctacattt agaatggaat aattagctta tttttgataa agtaacttct
     8941 caaaatcagc acttggaata ggcttacaat aataataacc ttgaattacc acacaatcta
     9001 aactcactaa aaattcagct tgttcttttg tctctacacc ttctgccact acatccatat
     9061 ctaaatcttt tgccatggct atagtgtgct ttaaaactat tttttctctg tctgtgattc
     9121 cttcctcatt aaagaaacca caatctatct ttaatgtatc aatattaata ttttttaaca
     9181 tatttaatga agaatatgct gaaccaaagt catccatatt taaaacaata ccatccattc
     9241 taaggtcggc tattatctta gtaaataatt ttatattttt aataaataaa ctttctgtaa
     9301 gctctaattc taaaaaagat tttggtatac tatatttatt tattaaactt aaaacaattt
     9361 ctttaaaatg tggattatgc aaatgtaatc ttgatacatt tacagagata ggaactggct
     9421 cttcacctaa atctatccat tttctaatca aagaacatac ttgttcccat acatacatat
     9481 ctaatctgat aataaaccca ttcttctcaa atagtgaaat aaatttaatt ggaggtatta
     9541 tacctttttc tggatgaacc catctaacca atgcttctgc accaacaatt ttatttgcat
     9601 taagattata cttaggttgt aaatatacat ggaattgccc attttcaagt gcgtattcca
     9661 tttccctttc catctgtttt tcttcaagtg catcatttct tatagtatca tcataaaata
     9721 tatagttatt gatactttgt ttctttactt ttttgtgagc atatcctgcc atttcacacg
     9781 catttgtaat tggaatatga ttatccaaaa ttctataaac cccaaaacat ggtactaaca
     9841 tatattcaaa tttttttcta tttaagctat ttttaatctt ataaattacc ttatgaatat
     9901 ctctatcaga gtaactctca taaaaaatgc aaaatacatc tgcatacata cgacaacata
     9961 taccattaga accaatgttt ttttgaatca actctgcaac atatattaaa gctttatctc
    10021 ctcctctaac tccataaatg tcattaatta ccttgaactt atctatatcc atcattataa
    10081 ttgcataacg cttgtcagga tatcttttaa gcattaaact tatttgttca caaaaacttt
    10141 cataattgta tagtcctgtt aatgtatctt tttggttttt atgtagttgc ataagataat
    10201 ctatattttt tatagcaact aaaatatgtg tttgtttctc acctaactta tttttactac
    10261 taatctttaa catataaatt aatgcccaat agtacttttt atctattcct aaacgtctat
    10321 actcaaaatt tagttctaaa tcgtcttgct tgctattatt aagactatca agagatttta
    10381 gaaggtggtt atcattaaat aatattatat catcaggatg aattaaatat ttttttatgt
    10441 atgaaaaagt tttttcaaga ttttccttta taggaaaact aaaaatattg ctctctgatt
    10501 gataaattgg agtaattaaa ttcgtatcaa tgcaaataga taaaattata tcatatgtat
    10561 tataagctaa tttttcataa gctttagaat acccttttat cattaaatca tcaaaataaa
    10621 ccttatcaac taactcttta tgtttaaata aaatattatt cgtcgaatca gctaagtcac
    10681 taattacttt catttcatta tttctaagtt tgttcttatt ataatacctc ttcttatctt
    10741 ggtacattct ctcatcagca actttaatta gcttatgtat atcaatatta tcgtcactcc
    10801 aaatactgcc cactgctaca ctatatatca tctcatcctt aaattcttca cttaaccaac
    10861 tcactaattc ttcaaatagt tgttttgata tatcttcaca taatacaaca aactcatctc
    10921 ctccaatacg gtaaacatta taagaatgaa agtatttttt tattattaca catgctgaca
    10981 caatagcaga atcgccatta gcatgtccaa agttatcatt aatatctttt aacccattta
    11041 tatctacaaa tacaactcct atggatttaa cttttccttt ttgaaaatta tctatatact
    11101 taatatattt atttctatta tatgcaccag tcaagaagtc atagtagctc atataattta
    11161 atttattttc tacttttctt tttttcatct cagttaataa aaaataagat agggatgtca
    11221 aaagagaggt atctccaaca gctttatgtg gattatcaac tccaataaac cctactactt
    11281 tatcatcttg ttttagtgaa gatgtcatta aactttcaat tttttgaggt tttaaaattt
    11341 catattctat tggctgttct tcttttatat tttctatatc atcaattatt acaaaacctt
    11401 tttgttcgaa taataaaaac caattcttta attcattaat atcaagattt gttagtgcct
    11461 gtttttgttc atcaacacct tcattgcacc attcatatgt attagttact ttttttctat
    11521 cttctgatac ttcaaaaaca tatgctctat cagctttatg aaatttacca atattggtta
    11581 atacaagatt tacagcatca gaaaaattag attctgttat aagatgttta atacatccaa
    11641 ctaataactc ctttatctct aatttttctg ctattgactt acttatatct tccttttctg
    11701 taatatcaag agctatttct aatcgtgcat atttgccctc ccatttaatt aacttgtcct
    11761 ttaatataaa atgtctattt gttcgagtat tcgtattttc ccatatgtaa tattcatctt
    11821 cttttaaaat agagtttgta caaaattcgc agggagaatc tttcccctgt aatgctttat
    11881 aacattttaa atgggatata tcattaagtc ccattgcctt tttcccaatt tcattaatat
    11941 ataaaagttc atatgtatct aaagaagaaa catatacaat ttcatccaac tcatctatta
    12001 aatttattac atccatttat atatatctcc ttttattcat tttccagatt gaatttttaa
    12061 agcttttaaa attttgctat atatttggca atgatattat acttctttat gttttaaaat
    12121 agtaccttaa tgaggttcta ttttttagta aatagaatta ttattgtgtc aatcgtacaa
    12181 tagagttttg taatacctaa tttaatatat ttataattaa ttatctcatt atgtgtttat
    12241 aattaattct catataaatg acaaatctcc acctcaatta agtattccaa tcatatatga
    12301 agataatact tcattcattt aaaaatataa aactattata tattaaaatt tcttcaataa
    12361 taaattatct agattgttac tttgatatat tatataatat cacaaaggct gatataaaaa
    12421 gacaaaaagg gcaatttttt tatatcaaaa aaaatgttaa attgatatta tccatatata
    12481 aattgatatt atccatatag gtgctatatt taacaattct acttacttcc cttacactta
    12541 ttagcatata gttattaaga gtttgttgca aaacaacact tagcacactt taaaaagtgt
    12601 ggtctaataa gtaatcaacg aagaagacat aataaagcat aaaatatgat atactttttc
    12661 acgccatcaa aactgaggat tttattatta atatatgcta aaatatgctt aataaattaa
    12721 attttaatgg gagaatagtt atgaaaatat ttagtttctt gttgtttgtg ataggaatat
    12781 gtttatgtat tgctggattt ataggacatt acccagtatt tgttggtaca acattgagtt
    12841 ttcttgcaat tgcaatgata gctgtaagac aagtcaaaac taagaaaaaa atgaaatagg
    12901 caaatcctaa ctttacaagt taatattaca caagtaatta gtctaaaatt ggatactttt
    12961 tctattacag aaatacatct tccactttgt attttcttaa aaaaataaaa tagctatgta
    13021 aattcaagat tagatagatt aactctctat ccatttcatt gtaaaatata catagctatt
    13081 tgtattcgat ttaaaatact tagattttct tacataacaa ataatttcta tctgattatt
    13141 actcatattc atttagtaat cactcagcaa ctaatcattt agtatctatt catccgccat
    13201 acggtaacct accccaacct cagtcaaaat atattctggc tgagcaggat tcttttcaat
    13261 tttccttctt atacttgcca taaaaactct aagcgactga ttttcacttt catagtaatt
    13321 tccccaaact ttatctatta taaacttatg ggttaagact cttcctgaat acttagacat
    13381 caactcaata atcttatatt cgattggagt taaatgtatt tcttcaccat taattgacac
    13441 aatatgattt tcataatcta tctttaggtt tttaacttca aaagactttg gagcatcatt
    13501 atttatatta tcctgttgag ctttatttct aagagccaca cgtactctag ccaaaagctc
    13561 tgttacacta aaaggtttag ttaaataatc gtctgctcct ccatctaaag cttccacctt
    13621 atccctctca tgctctctcg ctgaaacaac aattatttta ttagtctttg cacatgccct
    13681 aactcgtcct attacctcaa tcccatcaat atctggtagt cctaaatcca aaattatcaa
    13741 atctgggtca agagaatgta taagtgatat agctgttcct ccattatctg tctctacaca
    13801 cctataattt tgagtttcaa gagatacttt tataaattta caaattggct tatcatcttc
    13861 tacaattaga actaatggct ttatttccac ttgtctttcc cctcctcatc atacataggt
    13921 atgctaaatc taaatgtagc tcctccagaa ggattattaa aggcgacaat ttctccacca
    13981 tgagcttcta ctatagattt acatatctcc aatccaagac caatacctct tctttcaaga
    14041 tttctagact tattcttagt ataaaaccta ttaaaaatat tcccgatatc ttcatttggt
    14101 attccttttc cattgtctgc aacttcaaat agtacttgat ttgactcttt ttttacagtt
    14161 agttctatct tagaattttt aggtgtatgc cttatagcat tatcaattag atttactaac
    14221 acttgttcta ttaataatac atccacatga acgattatta tctcatccgg aatatttgtt
    14281 tttatgtctc tactattggc aaactttttg actcttaata tagattcagc tattatttca
    14341 tcaacaacct ctggtcgttt ttcaatatcc aattttcctt catctattct ggtcatacta
    14401 agtatattct caacagaatg agttagccaa ctcgtatctt catatatatt tttcaataat
    14461 tctactctag tttctttatc tatcacatca tcattttcaa gtattgttga agatgaaccc
    14521 aatatacttg taagtggagt tcttaaatca tgagaaattg acctaagcaa gtttcctctt
    14581 aatctctctc tctctgcctc aagatttgct tttttatttt tctcaaataa attttctcta
    14641 tcaattgcca acgcaacctg tgttgaaact gcttctaata gtatcttttc attttctgta
    14701 ataatatcta tcttaaaaca cgcaacacct ataacaccaa gaacatttga ttcttgacct
    14761 tttataggga aataatatgc actgctgttt ttacaatatt ttgtcccaat tccaactggg
    14821 ttacctgtcc taaaagcttc ttttatagct tccttttcta atactgaatg gaatatattc
    14881 tcacttccat cattgttaca aatataaata cttggcttac ctagattatt attctcatct
    14941 gcaatagtca ctattacagt tctatttaat atatttacta aattagaccc acaaaattca
    15001 ataatttgat ttttattttt tgctttaagc aacgttttgt tattctcata gagcaatctt
    15061 atcatttttt ctctcttatg agaaagtctc acttctcttt ttatcctaga agttaaagta
    15121 cttgttataa ttgctgccat tagcataata caaaaggtca taggataatc ttttctataa
    15181 gctaaaaaag tatataatgg atgcgtaaag aaaaaattaa atgatagcac tcctattact
    15241 gaagaaagta ttccaaaaaa atatccatag gttatacttg tagcaaatag tacacctaat
    15301 atgtatacta gtataatatt agattcatta aaacctacct tttcaaaaac tactgatatt
    15361 ccagtagcaa ttgtcataac caatatagtt tttatgaagt ccttaataat tatcttttta
    15421 ctgagatagc catcttcatc ataatcatac tcttttaagt cttcatgttg tagtcgttcc
    15481 tttttcattt actacaattc ctccttattg ttactgttta aattggctct attttatata
    15541 atacaatttt atcataataa attcaatcaa tacatttaat taatgtactt aaattactaa
    15601 aaggtgctgt cttaaaatga aaataaatct ctatttcaaa acagcaccta tatatttaaa
    15661 ttactaattt agtttttaca tacttaaatc aatagtttta ttcaatttca taaattataa
    15721 tacttcaatt atagaacatt tctatccaac aagcataaat ataaagtggg cacatatacc
    15781 tgcacataaa ccacctatag tatgcgaaag tatcgcctta ccagctaatg gtcttgcatc
    15841 taaagcatcc atcataccta tgtgtgtact taaatatcca ctccaacaca tacccattgc
    15901 tgtgaatact gctatatcat taggagttat agcgcctgat tttataaact ctggtactaa
    15961 agatattgct gctccaacag ctcctaatgc agttacaggg aatgctattg cctctggtga
    16021 tgtgaatcca aataatggtt ctattatgaa gctaatctta tcaccaatag caggtaataa
    16081 ttttatacct tcatatgcag ctcctgtgta aacagcttga cctgtagcag ggtctgtaga
    16141 tggtccaaaa gttaataaca taactaatgt acaaactact aaaactcctg gtattatagc
    16201 catacccatt tcaacaccta acttaccacc ttcaagtata gcatccaatg ttctttggaa
    16261 tacattacca tctcttattt ctctaaactc ttcaagtttt gcacctttat catttatttg
    16321 cttttcagta tcatttttag ggtcataatt ataatacttt ttagtaaaag tcaacataat
    16381 tctaacacta ataatactac ctattattgc cgcaacatta cctattatag ctggtagtac
    16441 atattcttta ccttgagcta tcataaaagt tgttacaata agtcccatac caaaggctgt
    16501 tcctaaatta cataaagcag gtacttgata cttcttaaaa tattgtgtaa atgttttgtc
    16561 tttagcaaat ggtataatag ctggattatc agataaatat gttgcaacta ctcctgctat
    16621 actagcacca ggtaagcccc atataggtct cataagaccc ttaaatatct tattaactaa
    16681 agatattact ccaaactctg aaagcaatgc actaagtgca cctgcaagaa ctgccatagc
    16741 cattattaaa aatactgtgt ctaataaaag cgcatgtgct gtactcatta ttactttaaa
    16801 catcattcct gcacccatga tgcttcctac atatccaaat cctaccgcca atagtacaat
    16861 aaatacgaat gtttccatac ttactgcctt gactcttctc ttatccgaca gcttttccat
    16921 cttgaaatct tccccctttt aattttaaat taaggttcct aacattggta caaatttttt
    16981 ggtacaaact gagttctaaa acttacactc gtaatgcaag ttgtcagttt tttgaaataa
    17041 aaaaaaatta cgtttataga ataagaatat cctctttaat ttggatactc ttattctatt
    17101 ttatgttatt atattatcat ccttttgttt aaaagtcaat tttttctatt tttgttgttt
    17161 aattttattt atactctttt tatatttttc attttctttt atctttgtaa gtactccata
    17221 taagttgact tttctaaata cagtagttat aaataccaat cctaatgcaa ttgaacttgc
    17281 acacgataat gtactcaccc catattctac agcttttata taattattgg tcaaaacaga
    17341 atacatagta ttatatattc cataaccagg tactaatatt attaaacagc aaacacttaa
    17401 tgtagttgct ggagtcatat aaattcttgc acataactca caatatatcg caaaacaaac
    17461 tgtagctata aaaaaacata agttttctgt tactcccata tttaaaagaa atttatatga
    17521 aaaccaagct atacttccac ctattcctgc tagtattaag ttttttcctt tcatattaaa
    17581 tagcactcca aagcttaagg cagtaaaaaa agctgctatt acttctataa acaaatgcac
    17641 ttatatcccc cctaacttga agtacaatcc aagtaccaag aacattccta ctgctataga
    17701 tacacctaca aaaaatactt ctgctaattt tgcaagacca gaaatcaact gtccatctaa
    17761 caaatctctt aatgcattag tcaaagcaag cccgggaact aagagcatta ttgctcctgc
    17821 tattaaatgg tctaacgtct ttatgaatcc gagctttaaa attaaaattg aacaagaagc
    17881 tataataact gctccaattg catttacaaa aaagtcatta agggaagatt tacttaaata
    17941 cataataaca aacttagtca gaatcccaac agtaaatgca gctataaatt cttccacatt
    18001 tcctccaaat aatatcgaaa aagttgctgc tgcaatacct gcaaaaaata ttgttattaa
    18061 atccgagtaa tagttatctt cacttatttc atctaaaatt ttctcacata attcaatatc
    18121 taaattattt atacttattg ctcttgataa tgagttaact tcatgaacca agtttaggtc
    18181 tactcttctt gagcttattc ttttaattgc tgaatgtaat ttcccatcta caaatacaga
    18241 tgccattaca gctgttggag ttgcaaatac atctacatgg tctattccaa aagattgaca
    18301 tattctagct atcgtttctt caactctata ggtttctcct ccactttgca acatagcttt
    18361 acctgcattt gaggaaaagt ttaaaattct atctatatcc acaaaacata tctcccattc
    18421 ttttatagta ttattcatat tttaatacaa gatattattt atttaaacta ttttaaataa
    18481 ttcatatcca atatttacat tttatgaaaa aacttgtttt ttatgcttgt caaaatcaaa
    18541 ttttaagctt gtttacacca taaaatggct gaattaatat ttgacaaaca ctttcaacca
    18601 tttatataac cttctaaatt gtataacttt ttactacaaa taaaattata tctcttttaa
    18661 ttctttctct tgagtattta gcttatagta acttcatatt tatatatgta caatataaaa
    18721 cattattttt atgatacaaa atataatttg taaatgtata tatgtattaa ctgtgtatat
    18781 ataaattatt cttaacctta tcagtatttt catatatctt catctatacc gaccatatat
    18841 taattacatt gaccatatat taataaatga tattacatag acttaagtat attaaaaaac
    18901 ttaaatttag ttatttaaca acataatact ctaatttaat ctataaaaat ccatacctaa
    18961 tataatgcaa ctcttatttt aacaccatct attacacaaa acaacatatt aaaaagtaaa
    19021 atacaaaata tccactataa accttatttt aatgctgaat atatattata atatatttaa
    19081 tgtagcttaa atatagttta aacataaaga aaggaaacgg tatatgctaa gaaaagataa
    19141 agttctatta tctctacata aattatgtga aaatatgact ttaaaagacc ttcaatcaga
    19201 taaaacagga ttcactacaa tagaaattgc taaccattca tctcttgata gaagtaatac
    19261 cagtaaagaa ttaaataacc tatacaatga aaaaaaagtt ctgaaaatat ctggtaagcc
    19321 aattttattt ctacatgcta gtattataga aaatttaatt ggccgtactt tgaacagtga
    19381 tgaattttct ataaataatt gtaatgagct acttaaatca agtaaaaatg actcagaaga
    19441 tattttttca acattacttg gacatgattc tagtcttaaa ctacctatag aaaaagctaa
    19501 atctgctata ctctatcctc caaaaggtct taatacgtta ctcataggac ctactggtgt
    19561 tggaaaaagt acattttccg aaacaatgta caaatatgct gtagaaagta aaatcttttc
    19621 ttctgaaagc aaattcgtag tatttaactg tgctgaatat gcagaaaatg ctaacttatt
    19681 attaagtaat ttatttggtt atgtcaaagg ttcttttact ggtgctaata aggataaatt
    19741 cggcatagtt gcacaggctg atggaggcat attattctta gatgaaatac acagattacc
    19801 accagaaggt caagagatgc tttttctttt gatggacaaa ggcatataca gacgattagg
    19861 agaatctgac attgtgcata aagctaatgt ctttattgtg tgtgccacaa cagaagatat
    19921 acattcttct ctgcttggga cttttttaag aagaatccct gttacaatca acctacctgc
    19981 tttacaggat aggtctttaa aagaaagatt atctcttatt aaatacttct ttattataga
    20041 atcaaaatac accaatgcaa ctataaaagt tcacaaagat gtaattaaat cattactcct
    20101 ttatgattgt tttggtaata taggtcagct taaatctgac atacaactta tgagtgcaag
    20161 agccttctta aattacaaaa ctggacttaa ggataatata gaaatagatt tatcccttgt
    20221 tcctgattac atctatattg gtcttttaaa attgaatgaa aatcgtagta atctaaataa
    20281 attatcagaa cttgattata tgctttttta tgaatttaat ggtaataact tagaagttga
    20341 atatacaaaa gaactatcta aatacaatat agatacatca aacaatggat ttaaatactc
    20401 tacagagatt ccttatctgg aaaatgatat aaggagtatg tttataaatt acatggaaaa
    20461 gtactctaca aaattacttt cgatatcttc agtaactcat tctgattata caagtaatga
    20521 gatttttaaa gtaatcaatc caaagattta ctatgctgtt gaacattctt taaaagaggc
    20581 tgaaaaaata tcgagaaaca aatacacaaa acaaacttat attgctcttt caatgcatat
    20641 aagtgcactt atagaaaata taaatcaaga cacatataat cctaagtaca atttatataa
    20701 tgataaaatt ttcttagata aagatttaaa tatagctaaa atagttttaa gtataattga
    20761 aaatgactta caaacatctt tacctaaaga tgaagtaaaa tttatatcca tgtttctaaa
    20821 tagcatgtat cttgaaccaa acaatcaatc tgaaagtata ggagtcataa tacttgctca
    20881 tggaaattca acagcaagca gtatgtgtga tgtagcaaat tcattgcttg gtacaaacca
    20941 ttgtaatgca attgacatgc ctcttgatgt aaaagtaagt accatacttg ataaaactat
    21001 agagttggta aaagaatgtg acgaaggtaa gggtgtactt cttttaacag atatgggctc
    21061 tcttatagct tttggagaac ttatttcaga aaaaacaaaa atcaaaactc gctcaattga
    21121 aatggtatct actccaatag ttttagaagc agtaagaaaa agtacactac cagaaatgac
    21181 tttggatgaa ttagctaatt ccttaagctc tttaaatcca tatattggac gagcagcaat
    21241 cgattcaaca gaaataaact atcctgaaaa gtcaagagag tatgtaataa taactacttg
    21301 tataacagga tatggttcag ctatgaaagt agcagacttt ataaattctt cacttaaaaa
    21361 tattagtaaa tacaatatta atttagttcc tcataatata gaaagtttta aaatgtataa
    21421 gaataaattt agtaatacta aaattctggc agttgttgga agtgtagatt ttaatgtagc
    21481 caacacccct tttatacaca tagaggaact actaaatgga tatggcctag atatgctaaa
    21541 caaaataata gaaggtacat tcaatgccaa tagtattaca aatataaaag aaacaaatct
    21601 atcatacagc agtttaataa taaactttat gacccaaaac ttagagcttt tggactctgt
    21661 taaattattc aactatgtca attcttcctt atttacaata atagaattaa tagatgaaaa
    21721 cttaaagagc aaatttaagc taggatatat actacattgc tcttttatga tagaaagatg
    21781 ttttaaaaat acaacttttc catataataa cattactgaa ttaattagta aaaatcatga
    21841 actatatagt ttaatacgtg aagcctttaa gccaacagaa gcttatttta atattttgat
    21901 accagacgaa gaaattgctt atataataga catgattaat gagtatctaa ataaataaaa
    21961 tttatagcac ttattttgtg catattaatt aatatatgaa atggaggacg agattttgaa
    22021 aggaaaaata aaacagttaa caatacttgc attaattttt atatttataa ctcctgtatt
    22081 tgcatttgca gatactccac cagtaccaaa ttcttcaagg gcagcactac ttatagacca
    22141 ggaaactaaa agaatattat ttgaaaaaaa cattgatgaa aaaatgccat tagcgagttt
    22201 aagtaaaatg atgacctttt tattggcaat agaagcagtt gataaaaatc aagttaaaga
    22261 gactgatatg gtgaaaatag acaagtctac agcttcagta ggaggttcta cttgtaaact
    22321 taaagatggt gatgaaatat ctctaggaga gcttatgcag ggtctaatgc tagtatctgg
    22381 aaatgatgct gctatagcca tagctaaaca tattggtaaa actgaaaaga attttgtaaa
    22441 tatgatgaac aagaaagcag aagaaatagg aatgattgat acgtattatt ttaatccaaa
    22501 tgggcttcct atatatactg acccagaaca taaagaacct cctattgaaa atatgtcaac
    22561 tgctcacgat atagttacac ttggaaagta tatgtacgac cattatgaaa atcaagttac
    22621 aagaataacc actatgcaag tatataatga tactaaaaag gattttacac attataacac
    22681 aaatcctctg cttgtttcag tacctggtgt tgatggaata aagacaggat atacagataa
    22741 tgctggatat tgccttgcat tttctatgat ggtacctaaa gatgctaaaa acgaaagaaa
    22801 tcatagattg ataggtgttg tacttggtga tggtaacaag aaaaatagaa tttcttcttc
    22861 tgctacttta ttgaaatatg gtaaagataa tttccattct aaaaagatag cacataaggg
    22921 tgatattata gagactcctt gtgttgatgg tattgatgat tttaagataa cagttaaggt
    22981 tgataaagat ttatatggtg ttgtttcaga taacgagaat atcaatccta aggtagtgtt
    23041 taaaaatatg aattatccta tccataaggg agatattgtt ggtgttgcaa agtattataa
    23101 tgattctggt aaatttgttg gaagtgttga tgtaaaaagt gaaagtaata taggatgtat
    23161 tcctttgaag gataagatta agattaaggt tgctaagata aataaaaaac ttgagattaa
    23221 aaattctgtt tgttttaaag catagtaaag catattatta aaagggtatg ttaaatttat
    23281 aaagactcca ctttgtggag tttttttgtt ttttacaaaa aattaaaata gtggcaacaa
    23341 ttgaggatgt gagggagagt tgccactatt ttgtataggg tattatatta tgtttttaag
    23401 ctagttttac tatcttctat atttatataa taactcttgt gtgtgaattt aattgataat
    23461 taatatgaaa attatgtgaa attttgattt ccttactagg tattcttcaa atattaatag
    23521 tttttttcat ctatttttcc atcttttata gttataattc tatcagcttt ctttgcaata
    23581 ttatcattat gagttatcat aactaatgtt tgtttgtact ctttaacaca ccattcaagc
    23641 atattcataa cttcatttgt agtcttacta tctaagttac cagtaggttc atctgcaaat
    23701 attactgacg gtctattggc taaagctcta gctattgcaa ctctttgttg ttgacctcct
    23761 gatagttcat taggatattt ctttatttga ttttcaatcc ccaacttatt aattaatccc
    23821 ataacatatt ctttgtcttc tttcttccca tcaagcaata caggaagtac tatattatca
    23881 tatacattta taactggtat taaattgtag ctttgaaata tgaaaccaaa ttcctgtctt
    23941 cttatttcag acagcttatt atcatttaat gtatataaat ccacatcatc taagtacaca
    24001 ttccctaagg ttggcttgtc taagcctgca acacagtgaa gtagtgtact tttaccagaa
    24061 ccacttgcgc ctacaatagc cgtaaattta tgtggctcta tctctaaatc aataccatca
    24121 agagccctaa cttcattctc atattttcca tatatttttt ttaaatcttt taccttaagt
    24181 attttcataa cgtatctcca ttcccattta aaaaatatat attcttagat taaattttcg
    24241 taaataagtt ctactaccat atctaaattc tttaattgat ataactatac atctttgtag
    24301 gcaataccaa gattaaatgc tctatttaaa atagtaaaaa aattatatgt atcataattc
    24361 ccaatatatg taaagttatt aagtaaagaa tattcccttc tggtatattt aaattagcaa
    24421 ttctattcat tttcttttat gccacttact atactaagtt tatttatcat tttattagaa
    24481 aaataaactg caatgataca aacgaaaaca gatattaatc caaatattaa aatttcatat
    24541 attggaattt caaatttaat agaattactt ataccatata tctgtttgtt aagtttgtta
    24601 gtttcagaaa ccattttata ataactgaaa ctagagatga ttccagccac aagcatgctt
    24661 aatactccat atatgatatt ttcttttatt aacattcttt ttacattttt cttagacatc
    24721 cctactgctc tgaatgtaca tatctcactc aatctcacaa ttatatttga tctaactata
    24781 aaaataatat ttatagacga tattaataaa acgccaaata cacaaatcat tgctaactta
    24841 tatgaggact tattataatg gtctccaaaa tgattacgta catatcttcc ctctatatct
    24901 gaaaacccac ttttttctat tatattattt atttctttta ttactttttt atctgttccc
    24961 ttttttatct tcacaaaagc tttattataa taattcatcc ctgttagttc ttttaaatcc
    25021 ttttcattaa atatcacttg aattccgcct gattgagatt ctccatcttg ctctgatata
    25081 tagctatttt tcattattcc agctattctt acttttgtat ttatatattt atactctcca
    25141 tctttatata caggagtttt tatatctatt aagtctccta gtttaggtga ttttataatt
    25201 tgagtattat ttgacaattc aattctagaa taaaaattat ttaccaataa aatattttta
    25261 taatcacctt ttgtataact atagacattt tctcctttat ctatatactt atctatatta
    25321 tttaatagct ctttattgta accccgaatt aaaactgtag gctcattagt attatttatt
    25381 tcagttcttt ttagctcatc caaataacct ttttctaagt cagcatttct tagatatcct
    25441 tttaaataaa tattagattc aatatctgtt acatcgtcaa tagcttttat ttcatttaat
    25501 aaagaattat cgtacttaga aaatgtataa tttgcattta ttggtgaata tcccaaataa
    25561 atatcaaaat tgccatatga tttacttgtt atattagctg agtctgactc ttttccatat
    25621 atgaattttg tagaagttag tctgcctata aataaaactc cacatagtga tatcgtaata
    25681 ataattatta ttgatgtaac cttattccta aatatatttt taaaagctat ctcatttata
    25741 attccaataa cttttttatg tgattttaac aataaaaact taggaatata atttaactta
    25801 tctgtctttt ttatagcatc aattatagat aaatttccag ctttatatac aggtactaca
    25861 gaggctaata taactgctat aattgacatg atgatagatt caattatagc agttgtattt
    25921 atgattaatt ctgcactttt atcgtatact atcataagac ctaagtaaga aaaaactata
    25981 cctccaagta aaccaattac tatacccacg gataaaacta caatactctg aataaaaaat
    26041 atctgtctaa tatttctctt gctagctcca acagctctaa gtaaaccaat ttgcttagtc
    26101 atatctagta ttattatgtt aaacatattg tatattacta atgttgcaca taataaaggt
    26161 agtaagcttt gctttattga ataagttgtt ttttttgaaa tatttttcat atacatagac
    26221 atacttactt ctgtatttgg ataaaattga ttacttgtta acttatattt tctccctaga
    26281 ttcactactt tttgagtatc taaatctttt gtattaagtt ttaatgtacc catatgagtt
    26341 attaattcat atggaacaat atctttccca tcttttgaat atgtaaaagc tcttacctta
    26401 tatttattta aaccttcata gtattcatcg gttttactta aaataccaac tatcttaaat
    26461 tgcttatctt tactataaat agaattttca ttatttttat ttacatactc attaacaatt
    26521 tgaaaatcaa tagtttgatt taatttaaca tctagtttca ttttatctaa aactttttgc
    26581 tctattacta attcattaga atttctaggc tctctacctt caattaattt atatctagta
    26641 gaatctaaaa aatttttatt gtaagagcac aaatttatag atgacccatt atctttactt
    26701 acaatttctc caaatttaag cacattatta aattctttta tatcttcttc actattaagt
    26761 ttttttacta tcttactatc tacatcttcg taaaaagcat gataatctcc tgagtttttg
    26821 tatgcatgat ttatatctga ttgaccttga gattctttaa taacgcttaa tgaaaaaact
    26881 agtataacag caatccctat actaagtatc aatgataaag tttttccttt ttgttttttt
    26941 aaatacgaca ttgccagttt aatattaatc ataattaaaa ttctccttat ttatatttac
    27001 aagaaaaact ctatgatcta aaatagtaga ttatagagtc tcattaaaaa ctaactaatt
    27061 tattgtgcta gttaaattta attattaaaa atactagagc ctaaaaaaga atacttcatt
    27121 cctaaaatca tatctagtac ttttactatt taaatagatt gactagtctt ttttaatatg
    27181 ctactgtttc tttttaaatt tctcctgcat attcctacat acaatctatt tataaaatta
    27241 ttttataaat tataaatttt ctgttttttt aggattgatt actatattaa tctcactatt
    27301 tatcaactac ttaattgcta ttcttatatc actcatcata aatttctact aaacatacat
    27361 tttaaatcca tataaatata aatcacattt tttgttctat tattttattg agtactatca
    27421 tctctaaatc ttctatgctt aaaagatatc attttctatt tcatttttca ataccattgc
    27481 tccaactggg cactttaggg ctccaactag aattagttcc tagctaatgg tatataaata
    27541 gtaattgtaa acttattttt atctgaatat acttccacat ttccattata tttttctact
    27601 gaacctttca cactacttat tcctattcca tgtaaaaatg aatctttctt acttgtaata
    27661 agtttattat tttttaatac aacctcattt atctttgtat tttcacattt tattacaaat
    27721 aatctattta taacagttcc tcttagttta atctttttta gtatacttgt atccttaatc
    27781 ttattacaag cttctatcgc attatctatt atatttgaaa atatactaca tacatctggc
    27841 atctctataa aattacattc taaaaaattt atatctacta agaaatctat attattcata
    27901 tcacaaatag attttttttc acttaaaata acatctaaaa tcatactctg tgtattaaac
    27961 atagcattac attcttttat ttgcttattt atatctttaa tatatttatc tgcaaactca
    28021 tttttaccat aaatattctg aatacaaata acatgattac tcatatcatg atatagtttt
    28081 cttattttca actgatattc ctgtaaattt aaataatatt tatactgcat attcattttt
    28141 tcttttgtaa tctcatgttc tattcttaaa ttattatctt ttattattct tcctataata
    28201 cttattaatg aaatatttga tagtaaaaat acaattgata ttatgagtat tattaaattt
    28261 tctgtggaat caatattctt gtctttaaaa ataaattcaa atataactat cacaccaata
    28321 atatttgata ttattggaat agatgaatat atgcaatcct tcttgtttat ttttattttt
    28381 aatctgataa ctttaagtaa tggtattaat gctattagta atgatttaga tatcattatt
    28441 aattctaatt tcagcaaatt attatctaat aaagtagtca tattttgtat agagttaagt
    28501 gttcctacta tactcaatcc aataatatca aatccaatta atatcatcca atataacaaa
    28561 cttataaaca tacttttcca tttatctact atataat
//
LOCUS       DAEORZ010000040        28496 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEORZ010000040 DAEORZ010000000
VERSION     DAEORZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.040"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            18..668
                     /locus_tag="KRH39_001583"
     CDS             18..668
                     /locus_tag="KRH39_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBG8511497.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKGIVKKMGNTYEILNYDELHRLAFEVKEFLEDE"
     gene            complement(808..2184)
                     /locus_tag="KRH39_001584"
     CDS             complement(808..2184)
                     /locus_tag="KRH39_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8511498.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2200..4113)
                     /locus_tag="KRH39_001585"
     CDS             complement(2200..4113)
                     /locus_tag="KRH39_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG8511499.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDESGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4249..4881
                     /locus_tag="KRH39_001586"
     CDS             4249..4881
                     /locus_tag="KRH39_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBG8511500.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5020..5559
                     /locus_tag="KRH39_001587"
     CDS             5020..5559
                     /locus_tag="KRH39_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG8511501.1"
                     /translation="MIFVEELWKKAIEFHGHECPGLAIGFKAALAARKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(5736..5906)
                     /locus_tag="KRH39_001588"
     CDS             complement(5736..5906)
                     /locus_tag="KRH39_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8511502.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            6190..7257
                     /locus_tag="KRH39_001589"
     CDS             6190..7257
                     /locus_tag="KRH39_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBG8511503.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKEITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLENVKHVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSVANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPASITSSSSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            7427..9328
                     /gene="cooS"
                     /locus_tag="KRH39_001590"
     CDS             7427..9328
                     /gene="cooS"
                     /locus_tag="KRH39_001590"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG8511504.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAKMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            9321..9794
                     /locus_tag="KRH39_001591"
     CDS             9321..9794
                     /locus_tag="KRH39_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG8511505.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            9769..10995
                     /locus_tag="KRH39_001592"
     CDS             9769..10995
                     /locus_tag="KRH39_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG8511506.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENIYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLKISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVK
                     GVAFSNGEILDADMVIVATGVKPNADFLDETGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFIFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSV"
     gene            complement(11134..12321)
                     /locus_tag="KRH39_001593"
     CDS             complement(11134..12321)
                     /locus_tag="KRH39_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG8511507.1"
                     /translation="MNITKNFLKDYLERFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILNK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            12630..13454
                     /locus_tag="KRH39_001594"
     CDS             12630..13454
                     /locus_tag="KRH39_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8511508.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVRETIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            13583..14848
                     /gene="gluD"
                     /locus_tag="KRH39_001595"
     CDS             13583..14848
                     /gene="gluD"
                     /locus_tag="KRH39_001595"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBG8511509.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            15107..15697
                     /locus_tag="KRH39_001596"
                     /pseudo
     CDS             15107..15697
                     /locus_tag="KRH39_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
     gene            16028..17161
                     /locus_tag="KRH39_001597"
     CDS             16028..17161
                     /locus_tag="KRH39_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG8511510.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTIGNSIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDIDDEEIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGVEYYDSLSND"
     gene            17934..18956
                     /locus_tag="KRH39_001598"
     CDS             17934..18956
                     /locus_tag="KRH39_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG8511511.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSINQNVDSTNTNQQSNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            19253..20173
                     /gene="pyrB"
                     /locus_tag="KRH39_001599"
     CDS             19253..20173
                     /gene="pyrB"
                     /locus_tag="KRH39_001599"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBG8511512.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKVKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGISED"
     gene            20212..20904
                     /locus_tag="KRH39_001600"
     CDS             20212..20904
                     /locus_tag="KRH39_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBG8511513.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILENEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNEGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            20897..21799
                     /locus_tag="KRH39_001601"
     CDS             20897..21799
                     /locus_tag="KRH39_001601"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG8511514.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDNELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVHEVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            21884..22468
                     /locus_tag="KRH39_001602"
     CDS             21884..22468
                     /locus_tag="KRH39_001602"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBG8511515.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            22683..23609
                     /locus_tag="KRH39_001603"
     CDS             22683..23609
                     /locus_tag="KRH39_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HBG8511516.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGVIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYILSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVENSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(23661..24551)
                     /locus_tag="KRH39_001604"
     CDS             complement(23661..24551)
                     /locus_tag="KRH39_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8511517.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            24739..25770
                     /locus_tag="KRH39_001605"
     CDS             24739..25770
                     /locus_tag="KRH39_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBG8511518.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            26064..26939
                     /locus_tag="KRH39_001606"
     CDS             26064..26939
                     /locus_tag="KRH39_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HBG8511519.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDKVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            26957..28447
                     /gene="cls"
                     /locus_tag="KRH39_001607"
     CDS             26957..28447
                     /gene="cls"
                     /locus_tag="KRH39_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG8511520.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNIGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
BASE COUNT        11062 a         3176 c         4862 g         9396 t
ORIGIN      
        1 taaaggaggt aactttgatg aaagcagaaa aaacaagtat atatccaatt attaaaaata
       61 atttaaacaa aatatcaatt gttgaatata aaaagggtca aagatttgta gcatcagata
      121 aagacttaca agaagtgttc tttataatag agggagttgc attagtggag tgtactacta
      181 gaggaggaaa taaatttctt gttgacattg tacctgaaaa tgaatttata ggcaaaatca
      241 gttatatata tgaacataat ttgaaatgtg atatatttgc aaagaccaat ataaaattat
      301 ttcgatttga aaaaaatgta tttgaagaat tttatctcaa accagatttt attgcactat
      361 ttcacagaaa atgtacaaga agaatatatg aattatataa aactagaatg gtaagagaac
      421 tattttcttg cacagaagtt attgcctatt gtatatcaaa taaacaagaa aataatgttt
      481 gcaatataaa agctattcgt aatttcagtg aaatctattc aattagtaga aaaagtagat
      541 accattctct gttaaagtta actgaaaaag ggatagtaaa aaaaatgggt aatacatatg
      601 aaattttaaa ctatgatgaa ttacatagat tggcatttga agtaaaagag tttctggaag
      661 atgagtaaag attgttactt aatcaaaata aggtataata atttctgtaa aaaatagaaa
      721 tagtctcact gaaaatcaaa aatactttag ttattttcaa tgagactgtt tagaaacatt
      781 gtataattac aatgtatagt ataattatta gtcaatttcc tttactatat taatagccca
      841 ttttttagat aatgctctag gaacgccaaa tataaactta accacttctt cactataagt
      901 gagtaaaacg agtacaaata taggtgcacc tttaaatgca gctataaaag ttagaggaat
      961 agaagctaac cacatacatc ctaattctaa aaatagagca tatttagtat ctcctccact
     1021 tcttaaaata ccaattataa ccaatgtatt aaaagattta agagccatta gtattcccat
     1081 tataaagaat atcttagtta tatcagaagc taagctatca gaaacactaa acatttttaa
     1141 taataatggt atatttaaaa ttaaaataat tccaagtatt aaaccagctg aaaatacaag
     1201 tatagaaaac tttttagcat attcaatggc tcttttaata tggtcagctc caagttcatt
     1261 tccaagcata atagatgcac cagatgcaat acatacagct gtcattatga agaaattacc
     1321 tgtactggtt gctatctggc tagctgctat agcagaagta ccagctcttg catatgcaac
     1381 agaatacagg acagttccaa ttgcccatac agagtcattt acaaatatag gaaagctttt
     1441 tgatgaaaaa gattttataa aaattttatc aatagctttt agatttttta gaccaaactt
     1501 taatatatag tcttttttat aaaggtaaac ataagtaatc attaaaatta attcgcatat
     1561 tctagcaata acagtagcta gagcagcacc ttttacaccc aatgcaggaa gaccgaagtt
     1621 accaaatata aatccataat ttaaaataac atttgttaca agtgcaaatg cactacaaat
     1681 catacctaac ttaggatttc taacacctct tgaaccagta ctaaatacag tacttacagc
     1741 aattaatgga taactaaata ctgcgatact aaagtaatct atacaaagtt ttactacctc
     1801 agaatcatac gaaaatatgt gtataattgg ctttggtgtt agtaaagcag gaataacaaa
     1861 tacaaaactt aaaacaattg ccaatacaca agtaagacct gtgacttttc ttatattact
     1921 cacatccttc tttccataaa actgtgaaat aaaaactcca gcacctcctg tgataccaga
     1981 taaggacata ttaaaaagaa agaaaaattg gtttgcaact ccaactgaag ctacagatgt
     2041 ttcccccaaa ctacttatca taattgtatc tattacattt acagaagtag aaattaagtt
     2101 ctgtatgata ataggtatac ataaagatag aagtatttta taaaatcttt tattttcgtc
     2161 ttttaaagaa aatagttctt gcaaataatc ctctcctttt tacataaatt cttcccactc
     2221 atcatataga gaggcaaggt tattttctaa attaattttt tcttggctaa cttctttagc
     2281 tttatctgga ttagagtata cttcttcttg acataaaagt atatctaatt tttcaatttc
     2341 aatctctaac ttttcaattt ctttttctat attttgtctt tttattctat ttttcttttc
     2401 tatttctctt tgttctctct cttttctttt ttcatctttt atttgagttc tagttttttc
     2461 ttctttttct tctataatac tcatctcttg gatttgcctt tttttatcta tataataatc
     2521 ataatttccc agatattcag taattccact ttcatctaaa actaaaattt tatcaacaac
     2581 agtatttaga aagtatctat catgtgatat tgtaaatata gtcccatcat aattagttaa
     2641 tgcctcttca aggacttcct tagaatcaat atctaggtga ttagtaggct catctaataa
     2701 caataagttt gcatttgata gtataagttt aagtatggca actctagctc tttctccacc
     2761 actcaatgta gaaatcttct taaaaacttc ttcatctaca aataaaaatg cccctaacat
     2821 atttctcaaa gtagtttgag taagatgagt attattttcc caaatctcat caattattgt
     2881 gttatctaaa ttaagagttt tttgttcctg atggaagtag gaaacatgta cattagttcc
     2941 aaatttaatg ttgccactaa gaggttgtag ttcattagtt attattttaa ataatgtaga
     3001 tttaccaaca ccatttgcac ctataagtgc aactttttcc cctctatata tatctaaatc
     3061 caagtcttta aaaagtattc tttctccata acccatagat acgtctctaa tttgaagaac
     3121 atcattacca ctctggacag aaggcgtaaa ttgtatacga gcttttttcc tataagcttc
     3181 tggtttatct aaaacgtcta ccttgtctaa tgctttttct ttacttcttg cacgctttag
     3241 gtgtttttct ctaccataag cttttagtct ttcaattgat tcttcttgct tttttatttc
     3301 tttttgttgg tcttcaaatt tcttaagctc tagttctttt tctattttag ataactcaac
     3361 aaatttagag tagttaccat tatatacttt aagttttttg ttgtgaactt caaaaattct
     3421 atttacggat tggtctaaga aatatctatc atgagatatt aacatgacag tccctttata
     3481 ttgttttaaa aatacttcta accattctat tgcttcagag tctaagtggt tagtaggttc
     3541 atcaagtaac aatagagttg gtttcttaag aagtaatttt cctaaaagaa ctctagtttt
     3601 ttcaccacca gaaagtatgc ttattggctt atccatatca ttgtcactaa atcctaatcc
     3661 ttttagaact cctttggctt cagatttata tccatatcca tttaaatctg aaaataattc
     3721 tagtttatgt gaatattcat ccatgatttt ttctataatt ggagagtttg tttttgtact
     3781 ttcttcagat attttaacct ccaattctct taaataggat tccatttcta tcaaattttt
     3841 aaaaacttct agtacttctt ccaaaatagt attatccgaa taaaaattgg tattttgttc
     3901 taaatatccg atttcacaat ctttagaagt gtaaatatct ccactatcat atccataaat
     3961 tcctgaaatt attttaaata aagtagtttt tccagtacca ttaacgccga ttataccaat
     4021 tttatcgccc tcatttactg taaaactaat attttctaat atagaatcta taccaaaact
     4081 tttatttaag ttattacatg acaaaacaat cataaatttc tacctccttg aactatctaa
     4141 aattataatg ttttgatata atataatcaa ggttatatgt attagttttg aaaaagaaat
     4201 attttattgt gaatcaaaca aaaaagagga cacttttagg aggtgaagat gttgggaaat
     4261 aaaaatatat caatggcagt tataagaagg ctcccaaaat atcatagata tcttggagac
     4321 ttattagata gggatataca aagaatatct tctaaagaat tgagtgatat aatagggttt
     4381 accgcttctc aaataagaca agatttaaac aactttggtg gatttggaca acaaggatat
     4441 ggttataatg tagaagctct tcatactgag ataggtaaaa ttcttgggtt ggatcgacca
     4501 tacaacgcag ttcttgtagg agcaggtaac ttaggacaag ctatagccaa ttatgcagga
     4561 tttagaaaag ctggattcga gataaaagct ttatttgatg caaatcctag aatgataggt
     4621 ttaaagataa gagagtttga agtattagat tcagatactt tagaagactt tataaaaaac
     4681 aataatatag atattgctgt attatgtata cctaaaaatg gagcacaaga agttattaat
     4741 agagttgtaa aagctggaat taaaggtgta tggaattttg cacctttaga tttagaagtt
     4801 ccgaaaggtg ttatagttga aaatgtaaac ttaacagaaa gtttatttac tttatcgtat
     4861 ttaatgaaag aaggaaagta gacaacttaa acatattaat aatgagtagt tgtgaagagt
     4921 attgagaaag tcagaagatt taatattaaa tcctctggct ttttgaaata ttaggataaa
     4981 aatttaaata atgtatggaa ataaacaata ttaaggagtg tgatttttgt ggaggaatta
     5041 tggaaaaagg caattgaatt tcatggtcat gaatgtccag gcttagctat aggatttaaa
     5101 gcggcacttg cagcaagaaa atatttagga tgtaagcagt caggagatga ggaaattgta
     5161 tgtatttctg aaaatgatgc ttgtggaata gatggtatac aagtagtgct aagttgcaca
     5221 atgggtaagg gaaatttact acttaggatg actgggaaat cagtatatca tttctttaat
     5281 agagaaaatg gaaaatctat aagaatatat gtaaagtcaa agccatttga gatggataga
     5341 gaaggataca aaagatactt attagaaagt gattttgaag agatatttga gattacagat
     5401 actaagttaa gacttcctga aaaagcaaga atatttaaat cacttaaatg tgaagtgtgt
     5461 ggtgaatttg catctgagca atatataagg attgaagaag gaaagaaagt atgtcttgat
     5521 tgctttgata aatatgaaag attttatgag gagtattaaa attataaatt agattaaagt
     5581 taaaagtatt agatatgtgc taacagtatt ttaagatgaa ttaggtagca actataataa
     5641 aatatataaa taatgttact tgaatcatac ataattaaaa agaggctgat tagtatttag
     5701 aaaataaaat cagtctcttt atgaataacg aattattatt ctggttgagg tgcatctaca
     5761 ggacaagctc ctgcacaaga accacagtct atacaagaac cagcatctat aacatatgcg
     5821 tcgtctccag ctgatataca gctaacaggg cattcagcct cacaagcacc acagcttata
     5881 caagcgtcag taattttata tgccattttg aaatcctccc ttttatatgt attgtaattc
     5941 aataacatta taacaaatat tattgaatat aacaattaaa ttaattctac aacaaaaact
     6001 ttattatgat attcttgttt taaagtttta caataaagtt taatctgctt atatttttat
     6061 ttattttaaa gaagtttatt ttaaaagtaa aattaaattt aaaaagttat gtattgatta
     6121 tatttaaaaa tgttatactc taaaaagtat attttattta aaaatttatt aatacaaagg
     6181 agatttacta tgaaaaataa attagtttac atatgtatta tgagtctatt gctatttgta
     6241 gtaggatgtt ctaataatcc aaataaggaa agtaccatta aaaaagactc taagaataac
     6301 caaagccaag ttaaagaaga aaaaagagaa cctgtgaaaa aagagataac tataagtttc
     6361 gctggtgatg taactatggg gaactataaa ggttcatcat attatggcag ttttgaccat
     6421 gaatttaaaa gacaaggaga agattacaat tattttcttg aaaatgtaaa gcatgtattt
     6481 gaaaaagatg acttaacagt ggttaattta gaaggtccac ttactacagc gtcaaatgca
     6541 aaagaaaaaa aatttgcatt taaaggaaag cctggatatg taaatatttt aaaatctgga
     6601 agtgtagagg cagtaagtgt tgcaaataat cattcagagg attattttga agaaggaatg
     6661 aaagatacac agtttatatt ggacgagaat aaaataaatt attttggatt agggaaagat
     6721 gctgttgtag atgtcaaagg aattaaagta ggtctattag ggtataatgg attatcaact
     6781 gaatataatg aaaaaaactt aaaacaaatg gaagatgata ttaagtcctt aaaaaagaaa
     6841 tctgatgttg ttgtagtgta ctttcattgg ggaatagaat tagattatta tccagataaa
     6901 aaacaaaaag attttgctca ttatgctata gacaagggag cagatttagt tatggggtct
     6961 cacccacatg tgattcaagg aatagaaaaa tataaaaata agtatatagc ttatagtcta
     7021 gggaattttt gctttggagg aaataagaat ccttctgata cagatagtta tatatatcaa
     7081 caaaccttta ctttttcaga taataaatta acttcaatta aagagccaaa tataatacca
     7141 gcttcaataa catctagtag ctccagaaat aattatcaac caaaagtatt ggatggaagc
     7201 gagaaggata gagttctaaa taaacttgaa aaaataagca aagatttaaa taaataaaat
     7261 gtaaaaaaaa ataaatatat atatttattt taataattta tattttgttt agggtatggg
     7321 aaatcgtggt attaaataag tagattttaa tagttaaatt atagtcaatt ataataaagc
     7381 tatttaatct acttttttaa tataaaaata taagggggaa tttgtaatgg cttgtaacac
     7441 ttgtaaaatg tgcgaaagtg cagacaagaa attagaaagt tttgtagctt caaaggatgt
     7501 tgaaacagca tttcatagaa cagaagatca gaaagttaag tgtggttttg gacttcaggg
     7561 ggtttgttgt agactctgtt caaatggacc gtgtagagta actcctaaat caccaagagg
     7621 tatatgtgga gcagatgcag acactatagt agcaagaaac tttttaagag cagtagcatc
     7681 aggagcagct tgttatttac atgtggtaga aaatacagct aagaatttaa aaaatgttgg
     7741 aataactaaa ggtgttgtta aaggagaaaa aacattaaat caattagcaa agatgtttgg
     7801 aatagaaagt gatgaaaagt atgataaatg tataaaggtt gcagacaaag ttatcaacga
     7861 tttgtataaa tcaagagatg ataaaatgga attagtagaa aaaatagctt atgcacctag
     7921 agtgaaaaag tggaaagagt taggtataat gcctggtggt gcaaagtcag aagtttttga
     7981 cgcaatagta aaatcatcaa caaatttaaa cagtgaccca gtagacatgc tagttaattg
     8041 tttgaactta ggtatatcaa caggattata tggtcttaca ctaactaatc ttttaaatga
     8101 tgttatgttg ggagaaccag ttataagaat ggctcctgta ggatttaatg taatagaccc
     8161 agactacata aatataatga taacaggtca tcaacattca acatttgcaa atttccaaga
     8221 tagattaaaa gatgaagatg ttataaaatt agctcaatct gttggcgcaa aaggatttaa
     8281 gttagtagga tgtacatgtg ttggtcaaga tttacaatta agaggagaac attatcaaga
     8341 agtatttgct ggacatgcag gtaacaattt tacaagtgag gctgttttat caactggagc
     8401 aatagatata gttctttcag aatttaactg tactatacca gggcttgaac caatagcaga
     8461 taaatataaa gtaaaaatgg tttgtttaga tgatgttgct aaaaaagcga atgcagaata
     8521 cataggttta gacagaagta aattagatga acttagtaac actttaatag aaaaagcatt
     8581 ggagtcttat aaggagagaa gaggaagtat tgaaatagat ataccaaaag accatggatt
     8641 tgaacaatct ttgactggtg taagtgaaaa gaacttgaaa gcgtttttag gagattcatg
     8701 gaaacctctt attaatttaa ttgcagaagg aaaaattaaa ggtgttgcag ctgttgtagg
     8761 atgttcaaat atgacagcag gaggacatga tgtaaatact gttgaactta ctaaagaatt
     8821 gattaaaaaa gatataatag tactatctgc aggatgttct actggaggat tagaaaatgt
     8881 tggtcttatg tcacctggag cagaagaatt ggcaggagaa aacttaaaag aagtatgtaa
     8941 gactttagga ataccaccag tattaaactt tggaccatgt ctagctatag gaagactaga
     9001 aatagtagca acagaattag cagctgaact tggaattgac ctaccacaat taccattagt
     9061 gctttcagct ccacaatggt tggaagaaca agcattagct gatggagctt ttggtcttgc
     9121 ccttggctta cctcttcatt tagctttacc tccatttata acaggaggaa aattggtaac
     9181 agaagtatta acagaaaaat taaaagactt aacaggagga catgttatag tgaatccaga
     9241 ccctaaatct tcagctaatc aattagaaga aataataata gatagaagaa aaaatttagg
     9301 gttaaaggat gtggaatgca atgcatagaa tttttataaa taaagattta tgtacaggat
     9361 gtaagagctg tgtactagca tgtatgctaa aacacaataa agattatgac atgtacactc
     9421 ttgatttaga aaatatagat aatgatagta gagggcatat agaattagat agtaaacata
     9481 ataatccagt tccaattctt tgtagacact gtgatgaacc tgaatgtgta ttagcatgta
     9541 tgagtggtgc aatgcataaa gatagtgaaa gtggaatcgt ttcatatgat gaagagaaat
     9601 gtggttcatg ttatatgtgt gtaatgtctt gtccatatgg attattaaag ccagatgata
     9661 gaagcaagca aaacatactt aaatgtgatt tatgtaaaga tgaggagtat cctagatgtg
     9721 tagctaattg cccaagtgga gcaattgaat tacagaagga ggaaaatgat gaattatgta
     9781 gtgttagggg ctagtgcagc tggtataaat gcagtaaaaa cattaagaga attagataaa
     9841 gactcaaata tagtagtaat ttctaaagat gaaaacatat actcaagatg tatgttgcat
     9901 catgttatat ctgaacacag aactttaaaa caaataaatt ttgttgatga agattttatg
     9961 gagcaaaata atgtaaaatg gattgcaggt aagactgtta aaggtataga cataaataaa
    10021 aaagtagtcc agacagaaga cattactgta aattatgata aacttttaat agctacaggt
    10081 gcatcttctg ctattcctcc tataaaaaat ttaagagatg gaaactttgt atattctgtg
    10141 agaaacatag acgatattta taaaataaaa gaaaaagcac aaaattctaa aaatgtagtt
    10201 ataataggtg ctggtttagt tggtatagat gcactagtgg gattatttaa atatgagcag
    10261 ttaaagattt ctgtagcttt tatggaaaag tacattttag atagacaatt ggatgaatat
    10321 acagcatcag tgtatgaaaa taagttcaaa gaaaaaggtg taaaatttta cccaagtgca
    10381 tcaatacaag aaattgtatt ggataacagt aaaaatgtta aaggtgttgc attttcaaat
    10441 ggtgaaattt tagatgcaga catggtaatt gtagctacag gggttaaacc aaatgctgac
    10501 tttttagatg aaacaggaat agaatatgat agaggtataa taatagatga tatgtgtcaa
    10561 acaacacaaa aagacatata tgcagcagga gatgttgtag gtaaaaatgc tatttggcct
    10621 ctggcagtaa agcaaggaat tgtagctgca tataatatgg ttggaaaaga taagaagata
    10681 gaagatgaat ttgcttttaa aaatagtatg aatttcatgg atataccaac tatttctata
    10741 ggaatgaata cacctgtaga tgatagctat aaagtattaa ccagacatgg aattaatgat
    10801 tataaaaagt ttatttttaa agataatgta atctatggag cagttataca aggagatatt
    10861 tcatatgtag gtgtattaac ttatttaata aaaaataaag ttgaaatata tgatttagaa
    10921 aataggatat ttgatgtatg ttatgcagac ttttttaata taaaagaaaa tggagaattt
    10981 tgttatagcg tatagtatat acatggagta aggtttataa atacagtaaa tcaataaata
    11041 gagttattct aaaataagaa aaatgacaaa tcttatttag aaatagctct attttttgta
    11101 attaagttta aattatattc aatttgtaaa agattatttt gaacaattat acatatactc
    11161 tctagttaga ggtaaatcat tttttatgcc tttagtgaaa agtatttgat gtatatctat
    11221 aataccagtg ttgaaagcag cagcacaaga acaaaggtat aaatcccaca ttcttataaa
    11281 cttatcatca aacatttctt taatctttgg aagattctca gagaagtttt tttgccagta
    11341 tagtaaggtt tttacataat gtaatcttag actttctacg tctatagtat gatatttgta
    11401 atcactacaa atatgtatca tttctcttag gctaggaata actcctcctg ggaaaatata
    11461 tttttttatc caagggtctc cataagattc ctgcaatcca cttatatagt gaagtaaaaa
    11521 taatccacca tcttttaaaa cagcatttac gtttttaatg aaaagttcat agttttctct
    11581 accaacatgt tcaagcatcc caacgcttac aactctatca aacataaggt ctgatttttt
    11641 taattctcta tagtccataa gtcttacttc aaggtagtct tgaaggttct cttctttaat
    11701 tcttgcgtta aattccttaa attgctcttc gctaagcgta attccaacgc cgtgaatttt
    11761 atattttttt gcagcttcta ttagtaaaaa tccccaacca caaccaatat cgagaaggct
    11821 cataccttcc tctaaattta gtttatttaa tatgtggtga actttattga cttgagcatc
    11881 ataaagagta tcattatcat ttctaaagta tgcacaagaa taactaagtg tcttatctaa
    11941 ccataaacta taaaaatcgt taccaatgtc atagtgggca tgtatttctt tcttttgatg
    12001 tctcattgaa tttgatgagt gcaatagatg ttttacagct gaattatcaa tattgaattt
    12061 gtctatttgt tttaaaagta gatttaaagt taagaataaa tctccctcaa cttcaatgtc
    12121 tctatttatg taggcttctc caagtgctaa agatgtactt tttaatagtt cagacttgct
    12181 aatatcatta tttactataa ctttgaactg aggggagccc tctccaatta gaaattcttc
    12241 ctcatcttta agcctaacta gaaatggtgt ttcaccaaat ctctctaaat agtcttttaa
    12301 aaaatttttt gtaatattca tattaccagc tccttctaat gtaaatccag taagtttccc
    12361 tgcttaaaat atattatagt ccttttttta aaaaagtaaa attttttcaa taaatttttt
    12421 atttagagga aattttttat ttagaggaaa ttttttattt agaggaaatt tattttttat
    12481 aaagaaattt catgttgtag tatatttttt gcctataaat tttggtactg gatataatat
    12541 taaactgtta atgatataat aaatttaatc tataaagtaa ataaaatttt gtaataaata
    12601 gttaaagcta ttttataagg agatatatga tggaagtata tgctttggtt gggtcaagtg
    12661 gcacagggaa gagctttaag gctttagagt tcgcatatga aaatgatata gaatatataa
    12721 tcgatgatgg gattttaata tacaaaaaca aggttttagc tgggatatca gcaaaacaag
    12781 caaatactac tatagaagca gttaaaaggg ctatttttta taatctggag catagacaag
    12841 aagttagaga aacaattaaa aaagaaaata taagaaaaat tcttattatt gggacatcaa
    12901 aaaaaatggt taatcaaatt gtagagagat tgtcaattgg aagggtctgt aagtttataa
    12961 atatacaaga tataagtact aaatatgaaa tagaaatagc aaaacaagct agaaaagaag
    13021 gaaatcatgt aattcctgtt ccagcagttg agataaaaag tatggcaagt ggtcttagta
    13081 taaattcttt gaagagactt tttagaaaag gaaataatag gaatatggta gttttagaaa
    13141 aaacaattat aagaccaact tttagttata taggaaagtt ttatattagt gctgatgtaa
    13201 ttaaacagat tattgaatat gaagtataca actttggaag cattgataga ataaataaaa
    13261 tcaatataga gaattgcaat aattttatga atatttttgt aagtataaat atcaatgatt
    13321 tagaaactat aaaaaaaatt gaagatatac agcagacaat aaaaaagtct attgagaaaa
    13381 tgactttagt taatgtacaa aaaatagata ttaacattca taaaattaaa aaaataaact
    13441 tcctccaatg ttaaaaaata aactgagaaa atgatatact aatttgcaat aaagtgtgct
    13501 ataattaaac tgtaaattta ggaaatggtt ttttctaaat ggagtataca taattataac
    13561 gtatttataa gggggctttc aaatgtcagg aaaagatgta aatgtcttcg agatggcgca
    13621 atctcaagta aaaaatgcat gtgataaatt aggtatggaa ccagcagttt atgaattatt
    13681 aaaagaacct atgagagtta tagaggtttc aattccagta aaaatggatg atggttctat
    13741 aaaaactttt aaaggattta gatcacaaca taatgatgca gtagggccaa caaaaggtgg
    13801 aataagattc catcaaaatg tttcaagaga cgaagtaaaa gctttatcta tatggatgac
    13861 tttcaagtgt tctgtaacag gtataccata tggtggaggt aaaggtggaa taatagtaga
    13921 tccatcaact ttatctcaag gtgaattaga aagattaagt agaggataca tagatggaat
    13981 atataaatta ataggtgaga aagttgacgt tcctgcacca gacgtaaata ctaatggtca
    14041 aataatgtct tggatggttg atgagtataa taaattaact ggacaaagtt ctataggtgt
    14101 tataactggt aagccagttg aatttggtgg ttctttagga agaacagctg ctactggatt
    14161 tggtgttgct gttactgcaa gagaagctgc tgctaaatta ggaatagaca tgaaaaaagc
    14221 taaaatagct gttcaaggta taggaaacgt tggttcttat acagttctta actgtgaaaa
    14281 acttggtggt actgttgtag ctatggctga atggtgtaaa tcagaaggtt cttatgctat
    14341 atacaatgaa aatggtttag atggtcaagc tatgttagat tatatgaaag aacatggtaa
    14401 cttattaaac ttcccaggag ctaagagaat atctttagaa gagttctggg cttcagatgt
    14461 tgatatagta ataccagctg cattagaaaa ctctataact aaagaagttg ctgaatctat
    14521 aaaagctaaa ttagtttgtg aggctgctaa tggaccaact actccagagg ctgatgaagt
    14581 atttgctgaa agaggaatag ttcttactcc agatatatta actaatgctg gtggagttac
    14641 agtttcttac ttcgagtggg tacaaaactt atatggatac tactggtcag aagaagaagt
    14701 agagcaaaaa gaagaaatag ctatggttaa agcatttgaa tctatttgga aaatcaaaga
    14761 agagtacaat gttacaatga gagaagctgc ttacatgcat tcaattaaga aagttgctga
    14821 agctatgaaa ttaagaggat ggtactagga tttactctta ataaataata ggatataatt
    14881 atttaagata taaaggggct gtacatagta cagcccttaa attatgtagc ttacaatgaa
    14941 gtgagctatt tttattttaa cttataaata acttgataaa ttatgtaatt tagaatgcat
    15001 aaataagatg ggtgaaaatt aatggttgat ttaattttga atggattttt taccaccaca
    15061 tgattacaaa ttagaagagt tagattggat ttttagacat gaacttatgc attttaaaag
    15121 caaagacctt catttaaagt atctagtatt atttttaaaa atagtatatt ggtttaatcc
    15181 atttatatat ataatggaaa aatatatgga tttggattgt gaaccttatt gtgatgagag
    15241 agttttaaaa aattgtactg tacaacaaaa gcaagaatat gctctaacta ttgcaaattc
    15301 aatgaaaaga ggtacaaatt tttaaaataa atttgtagct gtattaaata aacaatctga
    15361 tattagaaaa agagtaatga atatgttcag tgaaaaacat aaaaatggca tcttaatggc
    15421 tttgattata agcttgtttt cttctattac atttttaaaa tttgattcaa ctagtattat
    15481 tgaagagaat gatttcttag agaggtcaag atatacgttt gaaggaaggc aagaagacat
    15541 gtttgtaaca atagatttta catatgcaga tgcacctata aaacttagaa aagagcatga
    15601 agaaaactgt agagccaatg aaatagtacc aaaagattca ggttcaatag aaattattcc
    15661 tagattttat aaagatttta tagatttaaa aaaataagtt agacttaata ttgatgttga
    15721 tattatgaat tttttatatt taagcaattg aaaattttat tatggattca attttatatg
    15781 tgtttaagta tgaaatttat agatagttga aataattaaa tatgattgat gcataggaat
    15841 cattactaag ctataattaa ttataaggga gtttaaataa attatgagtt acacaattat
    15901 aaaatgataa aaaatagtta tctgttacaa aaaatgtatc tttttaatta ataaagataa
    15961 tatgaaaact taaaaggtaa ataaacttaa atataaaata ctcttaaata agataggaga
    16021 aaaatgtatg tctaacttta ttctaaatgg gatttttaga acagttatag taagtagtct
    16081 aagcattctt ctgatattga ttttcaagaa aaatgtattt aaaagattta gcaaaaaatt
    16141 taattattat atatggatga tagttgttat taaattattt ttaccattta catattatac
    16201 tttcacaata aatatcttga gaagtaaaaa acatattaat attaataaca taaatttaga
    16261 gggatttaat aatgtttcaa ctataggtaa tagtattata ttgtacactt ggattataac
    16321 tgtagtagtg tatttaattt acactatatt taaatatata aaacttaaaa atttaataaa
    16381 tgacttatct tatgatattg atgatgaaga gattgtaaat ctttataaaa atatattaga
    16441 agaatttaat ataacaaaag atataaaact caaatactct tatgaagttg aaacaccagc
    16501 attttttaat tcatgtgtgc ttttgccacc acatgagtat aaattgaaag agttagattg
    16561 gatttttaga catgagctta tgcattttaa gagtagagac ctttatttaa aatatgtaat
    16621 cctgttttta aaaacagtat attggtttaa tccatttata tatataatgg ataaacatat
    16681 agatttagat tgtgaacttt attgtgatga aagagtttta aaaaatcgaa attcagaaga
    16741 aaaaaaggat tatgctttaa caattctaaa tgcaatgaga aaaggttcaa atacttcaaa
    16801 taaatttata gctggccttc ataagcaatc tgatattaaa aagagagtta tgaatatgtt
    16861 taatgaaaag tacaagaatg gtatattaat ggcattaact ttatgtttat tatcttctat
    16921 tacttttttg aaggttaatt caactagtat tgttaatctt aatgtattac ctaaaggatt
    16981 tgaagactta cgaactgtta gcagtatacg tttttcagtt ataggattta catatgcaga
    17041 tgctcctaaa aatatgaggg aagaatataa aataaattgt gaatcattag gtttgatacc
    17101 taaagactct gataaaatag aagttggtgt agaatattat gacagtctat cgaatgatta
    17161 aataaatact attttaacag ataccaatta taagttatat aattttaaaa aaacagataa
    17221 attcaggtgt aaaatacaat tgggaaaagt gtatgtcgaa cttcatttaa aatggaattt
    17281 tcaaagttgt tatactaagt agtttaagca ttcttcttat attaatcttt gataataatg
    17341 tatttaaaaa agttagtaaa aaatttaact attatatatt aatgatagtt actattgata
    17401 tattatctaa aaaaattaag tatgaaaaga ttactgctag gacaatcttt tcatacttaa
    17461 tttttgaata tttatattct ccaaaatttt catcttttta acttagaatt attagaattt
    17521 gagaatcact attaggaaat aggctttgat ttatgcaggg ttaaaaattt cacagaatat
    17581 ctaactttat actaaatcca tcaaaaaata tataccacat aaaagcgcat aaaaaaacac
    17641 ataaaacaat atttatgcgc actttaacct cgaatggtac aaaattcttt acttttacag
    17701 acccatatta tataattaag taaataatgt atatttaggt gacatattgg ataagctaat
    17761 ttatttattt tattaatata ataatactat aaaagttata taaagtggtt tacaaaaaag
    17821 ttacagatag agcttttatt aatgtattgt gtattagtat tttatcctta ggtacacttc
    17881 acctacaaaa tagaatcata agataattat gggaaaattt taggaggttt atcgtgaaga
    17941 aaaagatatt aataccagta tttgcatcag ttatggcatt atcagtaagt tcaattgtaa
    18001 atgcagatga agtaaatgat tcatctcaaa ataaggatga taaaacaaat actgaattaa
    18061 atttaggtga atataaagaa gtaaaatata aagttgcaaa aataaaagat ggagtagcta
    18121 taaagataag agaagaaggt caagttcaaa atatagctta ttctggagat gaatttactg
    18181 ttttaggaac tcaaggagaa tgggttaagg ttaaagttga agatggtgaa ggttggttag
    18241 ctactagata tgtagatata tctgaaggtg tagggtatac taacgctgat aaagtaaact
    18301 taagaaaaga taagagtgaa agttcagaag taattgaaga gctggaaaaa ggaagttctt
    18361 tattggtttt ggaagataat ggagattggt taaaagttaa agatggggaa acagaaggat
    18421 atgtaaaatc atcttatata tctgataaag ctcctgtaat agaagagcca caagtgaacc
    18481 caagtattaa ccaaaatgta gattctacaa atactaatca acaaagcaat gcaaatacta
    18541 atcaacaaaa taataattca aatgtgccaa cagctaactc taatgcagtt caagctgtac
    18601 ttaacttggc atattctaag caaggttgcc catatgtttg gggagcagaa ggaccaaaca
    18661 cttttgactg ttctggattt acacaatatg tgtatagaaa tgcagtagga aagaatatac
    18721 caagaacatc taaagcacaa tctaaatatg gacaaactgt aagtaaagca aatttacaac
    18781 caggagactt agtattcttc acaactaatg gttctggttc tgttagtcat gttggaatat
    18841 atgtaggtgg gggtaatatg atacactcac catcaacagg aaaaacagtt agtgtaacta
    18901 gtataaattc atcatactat acagcaagat ttgttacagc aaaaagaata ttataatcgg
    18961 tttgtaatat gacaaattga taaatacata aatatatata aacgctctat attatattta
    19021 atttaatcat ggaaacaaaa taatgattat attttaaatg tactatagag cgtttttgta
    19081 tcactttaaa tctaatcaag attcaagtta atatgactgt taaataggag ccttttcata
    19141 tgatgattat ttgtgcaatc ccttgacagg atagggtaaa ggtggtaagc tactaacagt
    19201 acaaaaatac ttttaatttt agttcagaga ggctaaaaaa ggaggacgaa agatgttaaa
    19261 atcaagaaat ttaatccaac cagaagactt ttcaatagaa gaaatagatg aaatattaga
    19321 attagctcaa aagataattg acaatccgtc aaaatattct cgtatatgtg aaggaaaact
    19381 attagctaca ttattttatg aaccatcaac aagaacaaga ttaagctttg aatctgctat
    19441 gaacagatta ggaggacgtg tagtaggctt ttcagagcca aattcatcct ctgcatcaaa
    19501 aggtgaaact ctaggagata caatgaggat agtatctggt tatgttgata taatagctat
    19561 gagacatcct caatcaggtg cagcttcaga agcagctaga tatacggaag tgccatttat
    19621 aaatgcagga gatggcaaaa accagcatcc aactcaaaca cttacagact tacttactat
    19681 aaaatcatta aaaggaactt tagaaagcca tacaatagga ttatgtggtg atttaaaata
    19741 tggaagaact gtgcattctt tggttaaagc tatggcaaga tataaaaata caaagtttgt
    19801 atttatagcg cctgaagagt taaaaatgcc agattatata aaagaagcta taaagggaca
    19861 tgcatattat gaaactaata atttggatga tgttatagga agcttggatg ttttatatat
    19921 gactagagtt caacaagaaa gatttgaaga taaatcagag tatgaaagac ttaaaaacta
    19981 ttatatttta aataaagtta aattagaaaa ggcgtctaaa gatatgttag taatgcatcc
    20041 attaccaaga gtaaatgaaa tagacataga tgtagatagt gatgatagag ctgtatattt
    20101 taaacaagct aaatatggaa tgtatgttag aatggcttta ataataaaac ttttaggtat
    20161 aagtgaagac taatagttat catataatag taaaacaaag gagattttgg gatgtataaa
    20221 attctggaaa atatatatat aggcgaagat atgtatagaa tgaaagtaaa aggtaatttt
    20281 gaaggtaaaa tgggacaatt ctatatgcta agagcatggg atacttatcc agtactttca
    20341 agacctataa gtatacatga tatagatgaa gaagggataa cttttcttta taaagtagta
    20401 ggagaaggaa ctcaaatttt gagtaaccta aaggtaaatg atactataaa attagaaggc
    20461 ccttatggta atggatatgc taaggttgat ggaaaggtag ctctagtagg aggaggaatc
    20521 ggtgtagctc cactatattt ggttgctaaa aatataaaaa attgtgacgc atatcttggg
    20581 tttagagaag atgttatatt agaaaatgaa tataagcaag tatgtaataa agtctataca
    20641 acagtaggaa atacttttgt aacagatata attgatgttg agaaatatga ttatatatta
    20701 acatgtggtc caactccaat gatggaaaaa ttagttaaga tggtagaagg tactaaaacg
    20761 agaattatgg tatctcttga aaaccatatg gcttgtggag taggtgcctg tttagtgtgt
    20821 acgtgtaaaa ctaatgaagg aaataaaaag acttgtaaag atggtccagt gttctgggga
    20881 gaggacgtga tttttaatgg gtaatttaag tgtgaaattt ggaagtgtag aatttaaaaa
    20941 tccagtaata atggcgtctg gaacttttgg atttggaaaa gaatataatg agatttatga
    21001 tatacaaaaa cttggtggta taagtagcaa aggtcttact ttaaataaaa aacctggtaa
    21061 caatggtatg agagttcatg agacaccatc tggaatgatg aatagtgttg ggcttgaaaa
    21121 ccctggagtt caaggattta tagataatga actacctttt tttagtaaac tagacttagt
    21181 cagaatagct aatgttggag gtgggacatt agaagactac ttattagggg ttcaaatgtt
    21241 aaatgataaa cctatagaca ttattgaatt aaatatatct tgcccaaatg tcaaagctgg
    21301 tggaatggct tttggtataa aaaatgaagt agcaagagaa gttgttcatg aggtaagaaa
    21361 cataacgaaa ctgcctttag taataaaatt atcacctaat gcagaagaca ttgtaggaat
    21421 ggcaaaagtt tgtgaagaag aaggcgctga tggagtttca ttagttaata cttttaaagc
    21481 aatggcaatt gatataaaaa atagaagacc agtatttgaa aatgtatatg ctgggttatc
    21541 tggaccagct ataaaaccta tagctttaag aatggtacat gaagtgtgta aaaatgtaaa
    21601 tatacctgta atgggaatgg gcggaataac taaagctaca gatgctatag aatttataat
    21661 ggcaggagct acttgtatcc aagttggaac tgcaaacttt atgaatccta gaataggtat
    21721 tgaaataata gatggaatta atgaatttat ggatagagaa ggaataaaaa gtttagacga
    21781 aataagagga attatataag gcagaataaa tagtatttta aagataaaaa atatagatta
    21841 aataaacaat aattttataa cattaacatg gaggaaaaat aatatgagta aagtagatgt
    21901 agtagatata ttaaagaaaa gtgatgcatt gttggaggga cattttttat tatcttcagg
    21961 aaaacacagt aatagatacg tacaatgtgc aaaagtatta agattcccag aatatgctgc
    22021 tgaggtatta agtacagttg ttgaacaaat aaaagactta gatatagact tagtagtagg
    22081 accagctatg ggcggagtaa tagtttctta tgagttagga agacaattag gaaaagaagc
    22141 tgtatttact gagagaaaag acaatacaat ggagttaaga agaggatttg aagttaaaaa
    22201 aggagcaaag ataataattg ctgaagacgt tgtaactact ggtaaatcaa ctatggagac
    22261 aaaaagagta ttagaagcct taggtggaga agttgtaggt gttgcatgta tagcagatag
    22321 aactaatcat gatataggta tgcctatata tagtgctata aaacttgata ttcaagttta
    22381 tgaatctgat gagtgtcctt tatgtaagga aggaaaatta ccagttgtta agcctggaag
    22441 tagagagttc aaggaattag gaatgtaata aaaacttaat tattcatagt gttacttaaa
    22501 aaaggtaaat tttttagagt aaaaacttgt aatataacat ataacttaaa aataaaaagc
    22561 agttagcctt ataaatgaag gttagctgtt tttttgttaa aaatatattt acaaaataag
    22621 agatagtagc agtacaatat aagtataagg taaaaagtag tatatattag ggaggggtaa
    22681 gtatgaaaaa tcaattaaat gaaaatcata aaaaggttgt attttttgga gtaggcgctg
    22741 taggagctac ttttgcagaa caatttttta actctaaata tgattttaaa attctttgtg
    22801 ataatgaaag aaagaaaaga tatttagaag aaggatttat aataaatgga aaaaggtatg
    22861 attttgatta tgtaactaaa gatgagtata agcaagaggc tgattttata attataggtt
    22921 taaaatataa taatttaaaa gaaaatataa aagaattaga tggattagtt ggaaaaaata
    22981 cagttataat gtctctgcta aatggagttg atagcgaaga gataatagga gaaagatttg
    23041 gaattgaaaa aatggtatat tcatatgtta ccaatataga tgcaaagaaa atcaataata
    23101 atattataca cactactaat ggggtaattg tatttggtaa caaagataat agtgaagata
    23161 gaaaaactaa tataataacc gaagtctttg atgatgtaaa tatagaatat attttatcaa
    23221 aagatattca acgagatatg tggtggaagt acatggttaa tattggtgta aatcaaactt
    23281 cagctatact tggtgcacct tatggagttt ttcagagttc tgagcattta agagaattag
    23341 caaaatctgc aatgagggaa gttgttgcta tagcacaagc aaaagacata tctcttacag
    23401 aagatgatgt ggaaaattca ttacatagaa tactagaaca ttcaaaagaa ggaagaacat
    23461 caatgcttca agatgtggaa gctcatagac ttacagaagt agatatgttt tctaagaata
    23521 tctgcaaact tggaaaaaaa tataacatac ctactcctat aaatcagact ttcttttata
    23581 tgataaaagt aattgaaagt agattttaaa gggtctttgt aagaaatatt gataattatt
    23641 gattttaaat tttattaatg ttatatacta tttaagttac taaatatttt taactcttca
    23701 atgaaaatag ttgctgcttt tgataaagtt atatcttttg gaaatatata tgagaaatgt
    23761 ctgttgtagc tgcttccaag ctctatgaca gacagctcat tgtttagaac aggtaagctt
    23821 gtcactagat tagagactat tgtaattcct aaattatttc taacagattc tttgacagca
    23881 taattacttc caaaaaccat catacttttt ggaataattt ctttaacact taaaaacata
    23941 tctaaataat ctctggttcc agaaccatcc tccctgacta tccatttttg gttttgaagt
    24001 ttatcaaaac taaaatcttt taataaatga ctcttgtatg gaagtgctaa aaccatcttg
    24061 tcttcaaaaa aatattcttg aataaatgaa ggggaagagc aagtgccttc aataagacca
    24121 atatctaaaa tataatcttt aacatgagaa gatacaattg atgtattttt tataaaaact
    24181 tctacatcta tgtcaggata ctttttagaa aataatgcta gaaaatttgg tagaatatac
    24241 tctccaatag ttaaacttgc accaatcttt aagtgaccag ttatagcatc tgaaacttgg
    24301 cttacatcat gataagtaat atttaaaaga tttaatattt ctttagctct tttgtacaat
    24361 atttgtccac tttctgttat aaaaatggtt ttttgtttaa tagacctgtt tataagcaca
    24421 actttaaaat aattttctag attttttatg tgtttactta cacttggttg tgatagattt
    24481 aagtattccc cagctttagt aaagtttttg tattcaacaa cagctacaaa tgtttttaat
    24541 tcttcaaaca attttttgtc tcctttacct aatgaaattt tacatttttt atatactttc
    24601 tacagataat tatactatat ataataacca ttaaaaacat taatcattac gatattatat
    24661 atttatttta cttatggtta ttacaattat atactattgc tatgaagaaa actatattaa
    24721 ttataaggta ggtatattat gttaaagaaa aaaagcagtt tgacattaaa aactataagg
    24781 gaaatattac caggactgtt tgtatcagta ttagtaggtt atattagtat atttatttct
    24841 attttaattc ctaaggtagg ggcagcatca atttctatat ttttaggtat gtttgtagga
    24901 aacctatttt taaatcaaaa ggtatttcaa aaaggttata aattttcaga gacagatttg
    24961 ttatcatatt caatagtact attaggagca acactcagtg tatctacctt aatagactta
    25021 aaagtttcag gaattttatt tataatattg caaatgacaa taacaataat agctgcactt
    25081 tatataggta aaaaactagg atttgaagaa aactttagat ttctaatggc aagtggaaat
    25141 gcagtatgtg gttcttctgc tataggagca acagttccag tagttaatgc gacagataaa
    25201 gaaaagggaa ttgcagtaac tattgtaaat gttactggta tattccttat gtttttactt
    25261 ccaataatat ctcaattttt atatagccat gaacttgtaa aaacgtcagc aatgataggt
    25321 ggaactttgc aatcaattgg tcaggttgta gcaagtggag caattgttgg agaaaatgta
    25381 aaagatttgg ctactatttt taagatagta agagtaatat ttctagttgt tgttgtatta
    25441 gtatttggac atatgaaaaa tcagtctaat agggaaatcc ttgaagaaga aaaggatgag
    25501 ataaaaaaga aaaaagtaac tataccatgg tatgtaattg gattctttat aacttgtgca
    25561 cttttttcaa tgaatattat accaaaagaa gtatctgtgt tgtgcaaaga aataagtaac
    25621 aaactagaaa taatagcttt agcagcaatt ggattaaagg ttaatgtaaa agatttagtg
    25681 aaacaaggga aagaagtttc tttatatggt ttatttgtag gtacagtaca agttgtatca
    25741 gctgtagttt taataaaaat atttatatag cataaaaata gtatttgtaa tattaatttg
    25801 attaattaaa ataatatgat actaataatt tataatataa ataaaaaatg aatttcgata
    25861 agtttaagtg ggtataaact tatcgaaatt cattttttat ttaaggtaaa aatttaaagt
    25921 aaaatattat tttacataat aataacctta taaattttga aattacttat ctttgtgtta
    25981 aaataagtaa aatgcacata ttagagctaa gtttttaagc aaataaacaa aatgaaaata
    26041 taaatattaa ggagatttgg tatatgaatg tttatgaaaa aggtaatgga gttatactag
    26101 aaggagtaac agattttgac ccagtacata tatttgagtg tggtcaatgt tttagatggc
    26161 acaaacaaga agatggctca tacactggag ttgcaaaggg gagaatatta aatgtcaaaa
    26221 aagagaatga taaaatatat ttaaataata ctaatttaaa ggagtttaac agcatttggt
    26281 ataattactt tgatttagga actgattata cagaaataaa gaataagcta aaaaatatgg
    26341 atgaatattt aaataaggct actgaatttg gatggggaat aagaatatta agacaagatg
    26401 gatgggaaat gcttatatca tttataattt cctctaataa taggattcct atgatacaaa
    26461 gagctataga aaatttatca agaaaatttg gtaagtatat aggtgaatat gaaggtaatg
    26521 aatactatgc attcccaaca ccagaagaac taaataaggc ctcacaagag gaaataagag
    26581 catgtcaaac aggttttaga gataagtaca taaaaagtac tacacaggca gttatagaga
    26641 ataatgataa ggtatctgaa tatacaaatt taagtacaga ggattgtaga aaagagcttt
    26701 taaaatttaa tggagtagga ccaaaagtat gtgattgtat tgcattattt ggtatgcaaa
    26761 aatatgattc ttttccagtt gatgtttggg ttaagagagt tatgcaagaa ttttatatag
    26821 atgaagacat gagtttacct aaaatgagaa cttatggaat cgataaattt aaagaaatgt
    26881 ctggatttgc tcaacagtac ttattttact atgcaagaga actcggtata ggaaaataaa
    26941 ttaaggggga gaaattatgg gagtaatagg aacaatattt ctgttctatt taattatatc
    27001 atatctagct ggagccataa tttctgtgat tatattactt gaaaatagag accctgcaaa
    27061 aacaatgtct tggttattga tgtttattat atttccaggt gtaggtttaa tgatatatgc
    27121 tatttcaggt agaaatatta gaaaaagaaa attatttaag actcaaaaac tagctaataa
    27181 tataaaagaa aagaaactat ttgatacatt agaaaagata acagaaatag ttgaattaga
    27241 aaaagaatct ataaaacaaa ataagctttt aagagatgaa gaggatggta gttatagaaa
    27301 aagggtaata aatatgttat taaagactgg tatgtttccc tttactaaaa ataataaagt
    27361 ggatgtgttt gtagatggaa atgaaaagtt taaaagactt atagaagata taagagaagc
    27421 taaagaccat attcatttag agtactttat catcaaagat agtgaaatag gtagggtgtt
    27481 aaaagaagaa cttataaaaa aggctaaaga aggcattaag ataagaattt tatatgatga
    27541 tgtaggatgc tggagatttt ggtttaatag aaaatttttc cgagaaatga gggaagtcgg
    27601 tatagaaatt gcagcatttt tacctacaaa atttcctata ataggaggga aattaaacta
    27661 tagaaatcac agaaaaattg ttgttattga tggaataatt ggatatacag gtggaataaa
    27721 tataggagat gaatatttag gcaaaaatga taagtttgga tattggagag atactcatat
    27781 taggattaag ggtatttctg tatacatgct tcaaatgaca tttctgattg attggtatta
    27841 tacgacaaaa gaagtattag taactaaaaa ttattttcca agtgtaggga atattggtga
    27901 aagtatgata caagttgttg cgagtggtcc agacagtgat tgggaagata ttcattatgc
    27961 ttatttttct gcaatatgtc aggctagaaa aaatgtttat atagaaactc catattttat
    28021 tccagatgaa agtctactaa aagctataaa gagtgctgct cttagtggag ttgatgtgag
    28081 aataatattt cctaaaattg cagaccacaa gatagtaaat atagcatctt attcatattt
    28141 tgaagaaata ttaagagcag gtggtaaagt ttatttatat aataaaggat ttatacattc
    28201 taaagtcgta ataatagatg ataaaattgc ttctgcaggt acagctaata tggatttaag
    28261 aagttttatg cttaactttg aggtaaatgc atttatctat gatgaagaag ttattagggt
    28321 aatgacagat gatttctttg aggatttaag tcactgtgaa gaacttaatt tggaggtatt
    28381 taagaataga aatatcatac aaaaaatcaa agaatctgta gcaagattat tttcaccaat
    28441 attataaaaa attattaaaa aaggtattga aaattatgtg tgattggtat attata
//
LOCUS       DAEORZ010000041        27805 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEORZ010000041 DAEORZ010000000
VERSION     DAEORZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.041"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(69..857)
                     /locus_tag="KRH39_001608"
     CDS             complement(69..857)
                     /locus_tag="KRH39_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG8511521.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            1225..2181
                     /locus_tag="KRH39_001609"
     CDS             1225..2181
                     /locus_tag="KRH39_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG8511522.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            2466..3431
                     /locus_tag="KRH39_001610"
     CDS             2466..3431
                     /locus_tag="KRH39_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG8511523.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLN
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(3629..5740)
                     /locus_tag="KRH39_001611"
     CDS             complement(3629..5740)
                     /locus_tag="KRH39_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBG8511524.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(6042..6749)
                     /locus_tag="KRH39_001612"
     CDS             complement(6042..6749)
                     /locus_tag="KRH39_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG8511525.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            complement(6948..8861)
                     /locus_tag="KRH39_001613"
     CDS             complement(6948..8861)
                     /locus_tag="KRH39_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG8511526.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            complement(8945..10036)
                     /locus_tag="KRH39_001614"
     CDS             complement(8945..10036)
                     /locus_tag="KRH39_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG8511527.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            complement(10042..11409)
                     /locus_tag="KRH39_001615"
     CDS             complement(10042..11409)
                     /locus_tag="KRH39_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBG8511528.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            complement(11363..12115)
                     /locus_tag="KRH39_001616"
     CDS             complement(11363..12115)
                     /locus_tag="KRH39_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG8511529.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            complement(12163..13071)
                     /locus_tag="KRH39_001617"
     CDS             complement(12163..13071)
                     /locus_tag="KRH39_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG8511530.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            complement(13093..13842)
                     /locus_tag="KRH39_001618"
     CDS             complement(13093..13842)
                     /locus_tag="KRH39_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG8511531.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            complement(13871..15046)
                     /locus_tag="KRH39_001619"
     CDS             complement(13871..15046)
                     /locus_tag="KRH39_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBG8511532.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            complement(15061..15900)
                     /locus_tag="KRH39_001620"
     CDS             complement(15061..15900)
                     /locus_tag="KRH39_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG8511533.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            complement(15903..17060)
                     /locus_tag="KRH39_001621"
     CDS             complement(15903..17060)
                     /locus_tag="KRH39_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBG8511534.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            complement(17076..17798)
                     /locus_tag="KRH39_001622"
     CDS             complement(17076..17798)
                     /locus_tag="KRH39_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG8511535.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWVKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            complement(17810..18883)
                     /locus_tag="KRH39_001623"
     CDS             complement(17810..18883)
                     /locus_tag="KRH39_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBG8511536.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            complement(18905..20611)
                     /locus_tag="KRH39_001624"
     CDS             complement(18905..20611)
                     /locus_tag="KRH39_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG8511537.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLSKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            complement(20657..22213)
                     /gene="murJ"
                     /locus_tag="KRH39_001625"
     CDS             complement(20657..22213)
                     /gene="murJ"
                     /locus_tag="KRH39_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG8511538.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            complement(22328..23383)
                     /locus_tag="KRH39_001626"
     CDS             complement(22328..23383)
                     /locus_tag="KRH39_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBG8511539.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            complement(23478..24164)
                     /locus_tag="KRH39_001627"
     CDS             complement(23478..24164)
                     /locus_tag="KRH39_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBG8511540.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            complement(24384..26408)
                     /locus_tag="KRH39_001628"
     CDS             complement(24384..26408)
                     /locus_tag="KRH39_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8511541.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKLIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAHHNTNLSEKTAGT
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            complement(26662..27774)
                     /locus_tag="KRH39_001629"
     CDS             complement(26662..27774)
                     /locus_tag="KRH39_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBG8511542.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKIKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGNTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKEDI
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
BASE COUNT         8769 a         4983 c         3023 g        11030 t
ORIGIN      
        1 tatttatcaa aattacattg atattttttt gatttcttca aaataatctt tttcatctat
       61 acatatgact atccaatttt tataacctct tttatcttat ctacaacgcc attttcagta
      121 tttctttttg ctctaaatct agcaatttgt ttcaaatgtt catttgcatt ttccatggca
      181 taactatgat aggcactttt catcatttcc acatcattta actggtcacc aaataccata
      241 gtttcttcat attttatatc taacattttt tgtacttttt ttatggcact ccctttattt
      301 gcaccttttg cagttaaatc aagccactcc actccagaaa tacatacttg agctctatca
      361 gaaaactttt caaagtagat attattatta tgttctgcac ctttgaagtc aaatacagct
      421 atcttcaata tgtctccttc tactttcgtc aaatcttcta caattttaaa ctttgcataa
      481 taagtactga cctcttgtat aaacgcttca tctttgcttt ctatataagc gtattttttc
      541 ccactcatta caattttttt cccttttata cttcttgttg tctctatgat ttcatttact
      601 aattctttat ttaaaggatt tgataatatt tcttttcctt tatatactac tagtgttcca
      661 ttttctgata taaacatcat atcatctttt atatcttcaa atttttttat aagattttga
      721 tactgcctgc cactagcagc agaaaacatt atccctctct ctcttaattt tttaaatact
      781 ttataaaact ctggatttat ttcgctcttt tcatctaaca aagttccatc taaatctgtt
      841 gctattaatt ttatcataca tttcctcccc caaattatta atttataatt attttttatt
      901 aatttttatc ggtttgaatt ttcaattctt attatacact aaaaatttta aattacaatt
      961 tgcacctttc ataagatgct ataactacat tcaggaaaat atttttttca atatacacat
     1021 tagtttataa aaatttaagt cttcatacaa ttatattcca ataaaatcaa taataactac
     1081 acactttctt ctataaaatg ctataaaaat aattatatta tgacaaactt atgctaattt
     1141 attgtgtttt ttattgctta tgtaacaaat ttttaatata attaactatg tgtaattaaa
     1201 aaatttagaa agtaggggat attattgtca ggactcaaaa agttcgttat atttctagcc
     1261 cttttagtta tagcatttcc tgttagtata tatggatatg tttttactaa attaaaaact
     1321 atacaagatg atagtataaa tacagaggcg cttagtagta atgactataa aaacgaagaa
     1381 aatatcacaa acatattatt gttaggtaca gatgcaagac caggagaaga agtttctcgt
     1441 tctgatgcca tgatgatact tactattgat aataaaaata aaagtcttaa gctaacttca
     1501 ttaaatagag atacttatgt taatatacca ggacgtggag aacaaaaatt aacacatgcc
     1561 tacgtatatg gacaagctaa cttattaata gaaactatag aagaaaactt ccaacttgat
     1621 atacaaaact acgccgtagt tgactttttc tcttttatgg atatagttga tgctttaggt
     1681 ggagttactg ttactgttga taagagtgaa ataagagaat taaataaatt tattcctgaa
     1741 acttataaat ggaataaaaa tgacaataaa ggttctctta aatatataag aaattctggt
     1801 gagcaaaaat taaatggata tcagacactt gcattttctc gtataagaaa aaatgatagt
     1861 acagatgaaa gagatagaag acaaagaagt gttatacaga gtttaataaa tggtgttaag
     1921 gatttaccag tcactaaata tcctaatcta gttaacacaa tacttccgta tgtaaaaact
     1981 aatatgaatc ctaatgaaat aatatcttta ggaaaagaac ttttaagtat aggaaattta
     2041 aatttgaaat ctatggagtt tcctctttct actgaaaatg gtagaaaaat aggtaatgca
     2101 ggctatgtaa taccttttga ggaatatgaa ctagatgcaa tgcatgattt catatttaaa
     2161 gatattatgg ttgaagatta gaaattccaa aacaaaccaa taatttgatg ataaatacat
     2221 acctcttaaa tgcaaattac tttgtattta tggggtgtgt ttttatttta aaatataaat
     2281 tacttttaaa aaatatatta tatatacttt taactatata tataggtcga taaattttct
     2341 tataaattat atcattgtct tttttttgta actgaaacaa gtattgaata tatctcaata
     2401 ctttaatata attaagtgta tgctattaaa aaatatacag gaaaaagaag aaagaagggg
     2461 tacttttgtc aaaattaaag aaatttgtta tactactggc tttcttggta gtaatattcc
     2521 ctataagtgt ctatggatat ttttattata aattatctgc tattcatgac tcgagcataa
     2581 gtagtgattt attgaataac aatgaccata aaaatgaaga tgggataatt aacatactac
     2641 ttatgggaac agatgctaga ccaaatgaag attcttcaag gtcagatgct atgatgatac
     2701 ttactattga taacaaacac aatgatatca aacttacatc attagctcgt gatagctatg
     2761 tagacatccc tggtcatggt aaacaaaaac ttactcatgc ttatgcttat ggacaagctg
     2821 atttattaat acaaacaatt gaagagaatt ttaatattga tattcaaaat tatgcttgtg
     2881 ttaatttcga atcatttatg tatataattg atgctattgg tggcgttgag gttactgttg
     2941 aaaaaggaga aatacgagaa ctaaataaat ttattcctga aacttataaa tggaataaaa
     3001 gtgatgataa aggtagcata caatacatta gaaactctgg aaagcagact ctaaatggat
     3061 atcaggcact atcttttgca cgtatcagac acaacgatac agcctttgct agagatggca
     3121 gacagagaca gattatacaa gctataatca agaaaacgga aactttacct gttactaaat
     3181 atccaggttt attagatgca gttcttccat atgttaaaac taacatgaag cctaatgcaa
     3241 tactttcttt aggagctcaa gttttaaaaa tgggcgactt aaatattaag caatttgaat
     3301 ttcctattga tgatgaaatt catagtacag gtggtatata tggaaaagct ggatgggttc
     3361 ttagatttga cccagatact ttagacatat tacacgattt tatatttaat gacatagagt
     3421 ttaaacaata aaaaattcaa cataaagttt attaaaaagt ataagattaa ttactaaaaa
     3481 agaactatat cgcataatta ttttcaagtt gctgtgtaca cttgattaca aaatgcatat
     3541 agttcttttt tattttctaa tttatcatga taattttcat tttaatctac atttataaaa
     3601 tactctgtat tttaaattaa cttttttatt acttaactaa gtttaagaaa gtgtttaatg
     3661 tgttttgatt tataccatct ccagcttcgt atactagagt agttttctta ggctctaaag
     3721 cagttttctg agcagatgtt acactatttc caagcataac tactggtgat tttaatttag
     3781 ctgctaaagg acctgatgtt aatgcatcta ccaattgatt acttttactt acaaacattg
     3841 atgaatattc agcatttgga tagaattttt gtattacttt tccatttgtt tcttctctat
     3901 tttctccagc tattctattt ttagatacat catttgtaat gaccttatcc agcttattta
     3961 ttactgaatc agatatactt agattaccac ctataaaata agcatctttt acttttaaat
     4021 ctgatatcca attgtaaact tcatcactta cattatcttt tgatactaat actattggtt
     4081 gcttttcttc tcctgctttt gaagctatag aaagagaatc tgcttcacct gtaccacttg
     4141 ttatatatag tttttctact ggattagttt ttactaattc ttttgctatc attaaagatg
     4201 tactatatct atctgaacca ccaactctat ttattgatac atttgacaat gtatctttta
     4261 tctcactctc aactttatct ccaatagagt tatttcctcc aattaaaata actttacttg
     4321 gattaagtct ttttaactca ttcttagtgg atgtaggtat attatcctta tttaccaata
     4381 gtataggagc atcatttgat gtagcaagtg gtgttgatgt tataccatca actatagagt
     4441 acccatttac taatactact gtatctgaat ttgtagccca acctttttta cttattgcta
     4501 ctgctgtgtc atatctttcg tcgcctctta atttctccac tttaacatta gatggtggag
     4561 taactggttc ttcactacta gaatataaac tctttaaatc cttttgtata gaagtactat
     4621 ttgctatatc tttagctgag aaatacatac taccttttat ttcactatac tttctattta
     4681 atgttacttg ttgtactatt tcttttgcta tattttgtcc accataactc gattgacctt
     4741 gcttatatat accttgtcct atgtataagt ctacatttgt tccttttact tcattagccc
     4801 accatgccac taatttagaa taatctgctg ctttaagacc aattggccag taaagttgtg
     4861 gaactacata gtcaatcaat ccttgtttta tccatgctct tgtatctgca tatgtaccta
     4921 cataactttc attacctgat gtatctgaac ctgttgggtc actactctta tttctccata
     4981 tacctgctgg gcttacacca aatacaacat taggctttat actctttata gacgctttta
     5041 catctctaag tagagtattt acattttctc ttctccaatc atccttattt tgtccttttc
     5101 catatttttg gtatgttgca gtatcattga aacttactcc tggatagaag taatcatcaa
     5161 aatgaactcc atctatatcg taattttgaa ctacttctgc tatactatcc actatgtact
     5221 ttctaacttc tggtaaacct gggtcgtagt aatacttatt tccatgttta actacccagc
     5281 ttggattttt ctttgctgga tggtttgctg gcaatttatc tatgctctca tctgccatag
     5341 ttattctata tggattaaac catgcatgaa attccattcc tcttttatgt gcttcttcta
     5401 ttaaaaatgg taatgggtca tatccagggt ctttaccttg tgtaccggta agatattcag
     5461 accatgggtt tatatttgat ttatataaag catctgattt tggtcttact tgaaccactg
     5521 ctgtatttat tcctacactt tttaatttgt ccaataaatc tgtgtattcc tttttttgct
     5581 tggcttcatt attcttagtc ttaggccagt ctaaattata aactgtagat atccaagcag
     5641 ctctcatttc cttatcattt gatgaatcag caaatgaact tacactacac atagtaagtg
     5701 tcattatcaa tgataatact aatatactta tttttttcat ttcatccccc ttaatttttt
     5761 aaatttattt tttctacaat atacttctta ttaagtatat ctaaaattca tattatctgt
     5821 caaatattga ataatatagt taaaatttat atttgatatt tgattataat atgaaataat
     5881 ttattataaa aaaaacagca aagaaattta tcttctttgc tgtttttata acgttcatca
     5941 ctattttaat attactaaac tcttaaatct ctatgtaaaa cattaaatgt aattatatat
     6001 ttaatgttaa tattcaatcc tatgtaaatc aatatctatt attattgtaa aacacgtgta
     6061 gcagttataa gtcttcttgc ataattagtt cctggagcca tgctagtaac ttttactaca
     6121 tctcctgttt gtggtgaatg tatgaattta gaatctccta catacaatcc aacgtgattt
     6181 attgaaccac ctgaaccaaa gaaaactaaa tctcctggtt ttaattctgc tcttgatact
     6241 ttctttccta ctgttgcttg tgatcttgat actcttggta aattaactcc tgctccattt
     6301 ttatagacat aagaagtgaa cccagaacaa tcaaaagaat ttggtccagt tgctccccat
     6361 ttatatggct taccttgttg cttaaatgct aagtttaaaa cattttgtac ctttgcatct
     6421 acatttccac cttgaatagt taaataatct ccatttaccc atcctgtaac attgtcaaca
     6481 ctttttattt taatccatcc atttgaatgt aattctaaaa cttcaacttt gtctcctgtg
     6541 tttatctttc ctactactgc atcatttgca ctagctccac ttcttacatt taaaaaatca
     6601 cactggtcta ctgttgcact cataggtgaa ttagttggct ctgctgcact agccgttatt
     6661 gctcctgctt ctactgtgac aactcctgct attaacacca tagctataaa ttttttcaat
     6721 atatgttttt tatttttaat attcatcata atctcaaata cctaatcctt tcgaaaattt
     6781 aacattttgt tccttcgttt ccaattccta attaacattc tatcttacat caaaataaat
     6841 tgcaaccttt tttgtaatct ttttgtaatc tttttgtaat cttttgtttc tttttagtat
     6901 atatttgttt ctttttgctc cccctggtta caaattagaa atatattcta cttttctcta
     6961 gttgttgcaa tttctttgat attagcatct tctggtttaa tataagttgg cactaattcc
     7021 ctcataatac catctatttt ttcaacctct tcattgttag taatttctct taaaagtttt
     7081 aagtattttg ttattttttc tctatcaaca tctattggtc taccaataaa aatcttttta
     7141 tgttctgtat ccaatagacc ttcttctgac ataagtagtt cttcatacaa cttttctcct
     7201 ggtctaagac cagaaaattc aatttttata tcaacatctg gctcaaatcc actgaattta
     7261 attaagtttt ttgctaaatc caatattctt acaggttctc ccatatcaag tacaaatatt
     7321 tctccacctt tagccatagc cccagcttgt atgactaagc ctactgcttc tggtattgtc
     7381 ataaaatatc taataatatc tggatgagta actgtcactg gtccgccatc ttcaatttgc
     7441 ttcttaaaaa gtggaattac actaccatta ctaccaagca catttccaaa tctaacagca
     7501 acaaattcag tctggctttc agcattcata gtctgtataa tcatttcagc agctctttta
     7561 gtagctccca ttatgttagt agggtttaca gccttatctg tagatataag gacaaatttc
     7621 ttagctctat attttgaaga caagccagct atgtttaaag ttccaaatat attattcttt
     7681 atagcttcgc cagggctcga ttccataaga ggaacatgtt tatgtgctgc tgcatgaaat
     7741 accacttctg gtttatattt attaaatatt tcatccattc ttttttcttc tcttattgat
     7801 gctattactg tctttaaatc taatttattt ttatattttc taagtaattc ttgttgtatt
     7861 gagtaagcat tattttcata attatcaact ataataagct ctttaggatt aaatcctgca
     7921 atctgtctac aaagctctga accaattgag cctcctcctc cagttattag gattactttt
     7981 ccctcaatgt aattacttat ctctcttaaa tttgttttta taggctctct acccaacaag
     8041 tcttctatct ctacttctct aatctgtttt atatcaactt ttccatctat aatttcgtaa
     8101 attcctggta ttgtctttat tttacagtta gtgtttttac atatatcaat aatctctttt
     8161 tttctacgct tctctatgtt tgctattgaa aagattatct catctacatt gtacttttct
     8221 accacatctt ttatatctga agttgttcct acaattttca cattatggat tattcttcca
     8281 attttatttt tatcatcatc aattatagcc actgctactt ttttaagttc tggatttctt
     8341 tttagttcct gtataaccat ttctcctgca tctcctgcac caattataag gatatttgaa
     8401 acctttcctc tagaatatgt cttgttcatg actattctta tagctctata ggctattctt
     8461 gcgccacttg taaacattat aataaatatt gtatttaata cataaaacat tataggataa
     8521 tcaaacccta ataatttatt tattatataa atcggaatta aataagctaa agttgccaca
     8581 aaaatagata taatttcttc ttctcctgca tatctccata atgtgttgta gcacttaaac
     8641 aatctaaatg atactatatg aagtactata tatatacacg ctgaaattat tatatcatta
     8701 gtggtaaatg catttaaatt acctgtcaag cttaaagaca ttcgaaacgc tactagtacg
     8761 ctacaaatat ctagaattaa tagtgttaaa tgtctaaatt tatatttttt aaaaagccta
     8821 ttaaaaaagc tttctttctc taaatctata ctttctcgca taactagaag ccctccttta
     8881 aacagcaaaa gctacgcacc taagcatagc caacaacatc tatcttttgc tgtatttttt
     8941 cattttattt tttatattta tcaaatatag aatcataaat tttttctata tcattaaagt
     9001 ttaaattaac ctcataattt tctctaacaa attgacgccc tttcttaccc atattaagtc
     9061 taagttcatc attttccatt agttttatta tagcattttt tatttcttct ggttgttttg
     9121 gttctacaat taagccagtt tcattttccc atacagattc tggatgtcct cctacatttg
     9181 taactataac aggtacttca catgcttctg attctatagc tgccactcca aatccctctc
     9241 ttaaagaagg aaatacagtt acatcaaaag aattaaaagt cttgcttaca ttttctggag
     9301 atattcttcc taaaaactga acatcatttt ctatacccaa ttctttagct aaatttatta
     9361 ggttgtccat ctgacttcca gagccaccta ttttaagaat tattttctta tctttatatt
     9421 catctttaag catcttaaat gcttgtatta aatattctat tccatatttt ttttctaaac
     9481 tctttataat tcctattaca aaagcgtctt ctttttctac ttccataggc ttaaacctat
     9541 ccatatctac tccaaaaaag gttacatcta catgtttagc tgtatattta ttagcctctt
     9601 tagccatatc tctactattt gaaaagatat agtctgcttt tctaaagtta tgttttataa
     9661 tttgtttttg tataaaacca ttattaggga aatcataaat atcagtaccc caaacagaaa
     9721 taatatatgg atgataccca agtagacttc ctataaatcc ataactactt gcatactgtg
     9781 catgcaatat atctggcttt atttcattta ctagcttctt tatctgcttt attgaaccta
     9841 agtatcccgt ttttttagaa atattttcat tttttaattc ttctacatta gaaccaaaat
     9901 tataaatttt tattccttcc atgtgacctc catttaaaga gattacatga atgtcatatc
     9961 ctttattttt aaaaaaacta caccatttta aagtatgagg atttgaccca tcagctaaat
    10021 aacaaacagt cttcataata tttaccttcc tatcgaataa tactcaattc ctaactcttc
    10081 catatcttta attttataac aatttcttcc atcaaggact aaaggatttt tcatcttttc
    10141 cttatactta tgtatatcat aatttaatac atctttccat tctgtcatta taaaacatat
    10201 ctctgcatct tctatcgcat catctacaga atcagcataa agtatagagt caccaaatat
    10261 tttcttagta ttatttacac caattgggtc gtatacagta actcttgccc ctctttcgag
    10321 caaatatttt atatttggta tagatggtgc ttctcttata tcatcagtat ctggcttaaa
    10381 agtcaaacct aaaacagcaa ccttcatatc ttgaaagctc tctactaact tagatgcctt
    10441 tctaactaat ttaagttttt gactttcatt aacttctatt gcagctttta ctgtttttaa
    10501 ttcataacca ttatgttcag ataaccagtg aagagctttt gtgtcttttg gaaagcaaga
    10561 accaccatat cctattcccg cctttaaaaa cttacttcct attctatcat cataactcat
    10621 tcctaaagct acatcttcta tgttagctcc tgctatttca caaagatttg caatatcatt
    10681 cataaatgat attttaagag ctaagaagtt atttgatgcg tattttatca tctcagcact
    10741 atttctattt gtaactatta taggttgatt aaaaggctta tagatttcct ctaatatttc
    10801 tctagcccat tcactttcta cacctataac gattcttgaa gcatgtagag tatcttttac
    10861 tgctgttcct tgtgataaaa actctggatt agatgcaagc tctactttta catcattttt
    10921 taggtttcta tttatataat cttctatctc ttcatttgta ccaattggaa ctgtagattt
    10981 tattactact agagtatctt ttgtcacagt ttcagctatt tgctttgcaa ctgcctttat
    11041 ataatctagg ttggctgaac catcacttct ctcaggtgta cctacagcta ttaatattac
    11101 atctacatct tcataagctt ttttatagtc tgtagtgtaa tctattcttc ctctgttgta
    11161 atttttaacc ataagttctt ctaggccttc ttcatatata ggtgctattc caagtttcat
    11221 tgatttaact ttttgttcat ctacatcaac acagactaca ttttcattgc ctacctctgc
    11281 tagacaaaca cctgtaacca aacctacata tcccgtccca gctatagcta ttttcatttt
    11341 tttacctcca agtatataaa acttatactc tttttttaat ggcattaaaa acatagaaac
    11401 aaaatacata tagacttttt ggtaaactta aattttcaac atttctataa gtattccaag
    11461 ttcgttttaa tgcttttatc ttgttacttg atatagaatt tccaactaat ctgtaattca
    11521 ctaaatcttc ttgaatccca tatgcatatt tttccttttt aagtaactgt aaccaggttg
    11581 cagtatcttg tcctcttctt accaaaggca tgctgaactc tcctacaatc tccttatcaa
    11641 tgaccactgt agaacatcct attatagtat ttctaagtaa tccttcatag tcaattttct
    11701 taggagctct tgcaacctta ttcgtttttg acccatcttg tctcatatat ctataagatg
    11761 taaaagaaaa acctacattt tcttttaaca tatattctat ctgaatctct aattttctac
    11821 tatcccataa atcatcacta tctacaaatg caataaatcg accttccgca ttcttaatac
    11881 caacatttct agatactgca gctccagaat ttttttcaag ctttatatat tttattctag
    11941 catcttcttt tcgaaaactt tttataattt cacttgaatt gtccttagaa caatcatcca
    12001 ctaataaaag ctgccaattt ttataagttt gattttggat tgactttatc gtctctgata
    12061 aaaactcttc tgaattataa acaggagtaa ttatcgatac taaaggctca ttcatatttt
    12121 tactctcctt atatagcaac tagcagtact ataccaacaa gtttacttag atactaattt
    12181 tctttgtcca atatatttgt aatatcctaa tgggaataat gctaaataca acaatttatc
    12241 agatactttt cttaattcac caaaataatg actatcagac aacaatgagt atctggctaa
    12301 atttatgtaa ttttttatcc acatatatgg atatctactt aatggatatg tattttgatt
    12361 tattaaatat aaaaattcta atgctctacc tcttaatgca tttttactta atatattctt
    12421 agtcaaacca tcatccaaat aatctcttat tatcataggt ttatttacac atataacttt
    12481 atagtattta gacatttcat tccatactac actttctggt aaaaatctca cttcttttct
    12541 ttctggaaaa ggaaatttta taagctcatc agttacaaat actatgcttt tatctccctt
    12601 aacgccatac ttaaaatata ggtctgcaaa gtgacatatt ttcttatctt caggcatttc
    12661 tgtccctatc aaatctccac ttggatatac acaaagtcca acaactccac taaatccttc
    12721 tttattctct aaagaatccc actcatcatt agcaatttca agagaatctt taattaaacc
    12781 atcatcacta tctacaatga aaaatagctc tccatctgca ttttttacac ctagatttat
    12841 tgcagtatgt tttcctccat tttctttgta tatatatctt atatttaatt tattttctga
    12901 tataaatttt tcaactaact ctttagtact gtcgctactt ccatcatcaa caatcaacca
    12961 ttcaaaatca ttgaaatcat aagtttgagc ttttaaatca ttgtacaaat tttcaagtaa
    13021 atgagctctg ttatacgtcg gagtaaatac agtaaattta tatttataca ttaattaaat
    13081 cttcctctct gtctaacttt ttttgattcc gtttattcca taattaataa agtagtatat
    13141 agctttgtat aaaggtattt tttctatatt tctatatata ttccaagtcc acatagcagc
    13201 ttttatttta ttaccactta gggaattgct agattttcta tataatgcta gaacttcatt
    13261 tattccatga gcaatatgcc cttttttcaa tatcgataac cataaaacat aatcttcatg
    13321 tcttacacca ctcattctta tttcaaaatc caatttagac ttatcaatca caacagtaag
    13381 acatcctaag atatttcctt ttaataatct tctataatct acattaggtg gaacttttat
    13441 tactttgttt aattttttat cattttcatc catcagttca tatgaagtaa atgaaattac
    13501 atagtcattt tctaacataa aatttacttg tttttctaac ttactactat tccattggtc
    13561 atcactatct aaaaaagcta taaattgtcc tgttgcctta cttagtgcta aatttctagc
    13621 attagagaca cccttattag tctcagtctt tatacatttt attctactat cctgttgcat
    13681 ataagatttg actatattag gactattatc tgttgagcaa tcatcaataa ttaacatttc
    13741 ccattcttga taagtttggt ttaatactga ttttatggtt gcttcaataa atttttcaga
    13801 attatacatg ggagtaatta tagatactaa attcttcttc atattacctt cctcatcatt
    13861 tataaaatat ttatttaatt ttacttaata cacaatcata tatcctcttg cagttcttat
    13921 tatcattata tctgaaaaac ttatttcttt catttttttg gttatccata acctcaaaat
    13981 tattttcaat aatatcaata agtttattca cggcttcatc attattataa gccaaagacc
    14041 caaacatttc actttttaaa tctatatatg aatcaacttt ttccatatat tcatccaaat
    14101 cgaattggta aaacaagata ggtctattca tatacaaaaa gtctatggct acacttgaat
    14161 aatcagttat atttgcagct gattttctga tttcatttgc tagagttacc cctttatcta
    14221 aaaacacaat tcgttttcct gttatatttt cttttatatc atctataaac tcatgcataa
    14281 gatgatgcat ataaattttt acatttatat ctttctcatc taatatttta tttaatttgt
    14341 catcatttaa aaaattcatt atatttttaa aataatctgt atctgtaaat tttcctgaac
    14401 taaattttat ccaatctctc catgtaggag tatataatat atctcgagtt tgtgcaactt
    14461 catttgttgg taaattgtca tatcttgcat ttccaattat ataaactgag tcatctaaca
    14521 ttccccaatc atttaccatg acatttcttt caaattcaga acttgctgta actatattat
    14581 agcttttata taggttatct ataatatttt tgaacttttt gttcataggt tttctttttt
    14641 taaaacttat agtcccatga tttaaaaaca cttttatcgt ttttttatga aaataattaa
    14701 gaacaggtac agcaaaattg tatggtgcta tatctgctga tggtgcatgt gaaaatacta
    14761 tagcttttga attgtaataa tacagatagt tttctacact tcctcttatt aattttttgc
    14821 ctggaatttt atcaaatact ttactgtctt tatttactac ccaataagtt tctacatcat
    14881 tatgttcttt tagcatatac tcataaaaaa actttgaatt atcattgtat atatcccctg
    14941 aatgtccacc tataagccag atatctctac cattaaactt atgcttatta aatggatata
    15001 taaaaaaagc caaaagcata tttattaaaa atttaacctt gtccataaac accatcctca
    15061 ttataacttg tactgtctaa atccattaat tcttcctttt atagtggcac tatgataatc
    15121 tctattggaa ctcaatataa gacctcctat atttcttaca agttttgata ccataacttt
    15181 tgcaaactta taattttttc tatagactat ttcctttttg caaagcgctc caaagcctct
    15241 tccatagtta taatcttttt gatacttttc tttagattta gaatgttttt ttgcaggatg
    15301 gtaaattata tcatttccaa agtattttcc tttaaaacct aaactcaata agtttaaaac
    15361 ataatctatc tcttcaccag caccaaactc tccaccaaca cccaatttct catcaaatct
    15421 tgtatagtct ttaccttcaa aatcaataaa aaaagttatg gatgttatag tatccaaaac
    15481 gttggaactt gtgatatcac aagttccatc atacattttc ttaaatgcat caactttatt
    15541 tgaatccata gttttgcaac tgtatatttt ataatcttta tttttattaa agaaatttat
    15601 aactttttct aatgtatcat tttcgtagac acaatcatca tcagggaaac ctataatttg
    15661 ccctacagca ttgtctatgc ctacatttct gttataactc aatccttttt tagagctttt
    15721 tatatacttt atatctaact tatcaatgta tttatcaact atttctttaa ctttgctatt
    15781 atcattttgg tctactacta taagttcaaa gtttttgtaa gtttgatttt ctaagctgtc
    15841 cataagtaag tatatatcat catatctatt taatgttggc attattaatg atattaacat
    15901 tactacctcc tacttttcat cttccacaaa tctatttccc ttttatcatt taatatcact
    15961 tcttttatta gtaatactac tgcaaaacta aatgaagctt ctaataataa attttctgca
    16021 aaagagtata gtaaaaatat aaataaagct actaaatatg cttttttatc tttttctata
    16081 aagctttcta ttcctttata catcaaatac ataaataata tacaagcaac tactcctagt
    16141 acactaatta tatatacata agaactatct aatggattag ttgcttttat agcaggacta
    16201 tatgaatttc caaaaggttt tagaaagttc cccttttcag ccaagtatac atgccagaac
    16261 ttaggtctac ttgctaataa tctatttaat aaagtgcttt tatcaaatac tgtaccaata
    16321 attacactaa ataaagtaag tattattggt aatgtcttta ggagcattgg tagtcctttt
    16381 atttttttta tatctatcca tcttattatt tctacaaata ttaatcctgc taatatagtg
    16441 aaaaatccag ttctactata tgtggtttga taaacaaaaa atgcagaacc aaataaaatt
    16501 attctatccc atttattata atctttatat cttaagaata tatatgctgc atatatagta
    16561 accatatgat aaaatacact atttggatta ccaagtccaa aatccattct tgtaacaagc
    16621 atatctattt tttctatata tctatagtgg acttcctgat taaatatcaa tgaaggaaat
    16681 aaattaaata ataatatagc taccattaaa aaacatgctg atattattaa ataattttta
    16741 actataaatt tatcatctat ttgcttagct gatacagcta ataaaacgat taataatata
    16801 gctccttccc tatttaataa ataaagtaag acacaaactc caactagtac catttctgtt
    16861 atactttttc tcttatcaaa taaaaaaagt agcattagta ttcctattga agaataaaat
    16921 aacgctgatt taatcatccc tatatccata tttgggtcaa taaatgatat catatacttt
    16981 ctaagtaata tgataaacaa aacaaatata taaagatatt ttctaatcat aatatttcta
    17041 tcatcattta aagtcttcat tttactttct cccttttagc ttcgtaaagt attacttgca
    17101 aatattttac aacacttcca tatcttattt acacctgtta agtgtttgct ttctaagaaa
    17161 taatttctct ttttaaccca actttttccc caccaatgta tcccaaaagt gttttctgtt
    17221 atacattctg gtgtaaagtc ttctttaaat ccaaaaggat aaaaatattc tttgtcgtaa
    17281 acacgtattc cttcttcata attatttggg tcaatcttct tataattctt ttccataaga
    17341 tgagtcatta tctttggtat tgtaaacaat gaagaagtaa gtacttcttt ttcataatac
    17401 tccaataagt cttttaaaaa aggatgtcct ttttctacac ctattatagc accatttata
    17461 tactctctat catcttcata cccacagatt agcctattat tttctaaaag aggagttata
    17521 tcctttaata tttgcatatc tgtatccata taaactccac cttgttcata gagtactttt
    17581 attcttgtat aatcagatat gaatgcccaa agttttcttt tataacattc ttccaaaaac
    17641 ttatttcctt ttatttcttt ctctatatca aagttttttt cattccactc tactatctca
    17701 tattctggaa gcttttcttt ccaagaattt atacatatat tttctatatt tccttttgga
    17761 ccaccaaacc atacataatg tattttttta ggaatcaaat gaacacctcc tacatatatt
    17821 cttgtctatc ttcttctttt aactgctcta ctattttttt gacatcttga gctttatctt
    17881 ttctacaaac cataactgca tcatttgtag atactactat caaatcacta atacctattg
    17941 tcgatattaa tttgtcatct gaatatataa ttgaattttt agtatctata gtaatatttt
    18001 cacctctttt gatattacct tcatcatcag ttggatatat tgcacctaga gaatcccaag
    18061 aaccaacatc attccatcca aaatcaccag gaactactat aacatcatta cttctttcca
    18121 taataccata atctatagag attgattgta tagttgggta tatttctttt attttttcca
    18181 tttcttcttt tgttcctaag tatttactta tatcttctaa tttttcatat acttttggca
    18241 agtatctctt aaaatcttct agtattttag ataccttcca tacgaacatt ccactattcc
    18301 atacatactt tttagagttt acatattctt tagcaatttc atagtttggt ttttctacaa
    18361 actccactac ctcatatgca acatcttcta tagtattttc tctattaaaa tttatgtatc
    18421 catatcctgt tgatggaaat gtaggagtta tcccaatagt tactagttta tcattatttt
    18481 ctgctatata gatagctttt tccaaaatag atttaaattc tttttcatct tttatataat
    18541 ggtcagctgg atatacacac ataacaccat caccatattt tttcattatg ttaaatgctg
    18601 caaagcctat agctgctgct gtatttctgg cacatggctc tggcaatata ttactatcca
    18661 aacatttatc ttttactata tcctttaaag cttccaattg cttctcattg gttactataa
    18721 ataaatcatc tttttttgct agagaatcta ttctgtttat agtttcgttt attaaagcat
    18781 cttctccact taaatttata agctgtttag gtacttcttg tctactaagt ggccagaaac
    18841 gagttcctcc tcctccggcc attatgacat tgtatacttt cattgtttat ctccccttag
    18901 ctttttatat agaatccact acatttaata tataagatat taaattgttc aacttaaatt
    18961 ctacatcttc ttgattgtct gaatttgctc caaagtaaaa tttaatttta ggttctgtac
    19021 ctgaaggtct tatagctatc caagaaccat cttcaagtaa aaatttaagt acatctgact
    19081 taggtaaatc atcaacacca tctttatagt ctaaaatttt atctactttt acatcagcca
    19141 cattatctat tttaacactt ctaaagtaaa gcatcatctc ttttattttt tttattccat
    19201 ctatgccttt taaagttact gactttaagt cctctttgta gtatccaaat ttattatatg
    19261 tatctatcaa agcttcatac aagttcatac cttttgaaga atagtatgct gccatttcac
    19321 atatcataag tgaagataca acaccatcct tatctctagc atgagttcca attaagtaac
    19381 catagctttc ctcatatccc attataaaac ttctatcatt gctttcttca aacgctttta
    19441 ttttttctcc tataaattta aatcctgtta aagtatttaa acaatctaca ttataagcct
    19501 ttgctatttt tgcacctagt tctgatgtaa ctatagtttt tattattgta ggattgtttt
    19561 tagaaagttt attttcttca actaaactct ctaatatata tctaactaaa agtgaaccga
    19621 cttgatttcc atttaagact acatattcac cacttgaatc ttttacaacg atacctactc
    19681 tatcacaatc agggtctgtt cctattatca aatcagtgcc attattcttt gccatttcaa
    19741 tagctatatt aaacactgac ttttcttcag gattaggata ctttactgtt gaaaaattag
    19801 aatctggcaa ttcttgctcc ttaactacca ttacattttc aaagccacat tcatttaaga
    19861 cttttcttac tggcttattt cctgtaccat gaataggtgt atatattatt tttaattttt
    19921 tcccgtattc atctattata ttttgtctta aaacttgctt cttaacagct tttataaact
    19981 catcatctac ttcattatct aatatagtta ttaaattttt agataaagct tcttcaaaat
    20041 ctatactttt aatagtactg tagtcatcaa tcttattaac ttctgctatt atatcatttg
    20101 ccatatctat acagatttga ccaccatctg agtcatatac tttatatcca ttgtattctt
    20161 taggattatg acttgctgtt attactattc ccatagcaca ctttaaatat ctaacagcaa
    20221 aagaaagttc tggtgtaggc tttaattctt caaaaatata agcctttaca ccataagcac
    20281 ttaaagtctt tgctacttct atacaaaact ccctagacat gtatctacta tcatgtgcta
    20341 taactattcc cttttgctta ccttcttctc cttgagtttt catgatataa tttaaaacgc
    20401 ccagtgtcgc acgtctaaca gtataaatat tgattctatt agttcccgct cctattatac
    20461 ctcttaaacc acctgttcca aactctaaat ttttataaaa tctatcttgt atttcttttt
    20521 catcatcttt tatacttaat agctcatttt tcgtttgttc atcaaaataa ggagaattta
    20581 accacatctc ataattattc ttataatcca tattttcatc ctttctgact ctaatttaaa
    20641 atttcaaggt aattatctat cttctaagta gtttttgttt tccttttctt aaaaattcaa
    20701 aagctaaatc catctcttct accttaaata aaactatcaa tacagtatat acagaagctc
    20761 cacctaatac tccagctcca actgatataa tttctttaat tgttcctgaa cccataaatg
    20821 catatagatt attatatatc aataaagtag ctactgccat cacaccagat gctaccaaac
    20881 tttttagacc agtctttata attttatccc ctccaaaata tccatttttc tttttaagag
    20941 atataaataa taatataact gtaattatgt ttgatgtact agttgatata gcaatacctg
    21001 catgacccaa tggtcttatc aatattaaat taagaactat atttattatt aaagctatac
    21061 ttccatttat cattggcgtt tttgtatctg aaagagagta aaatgcccta tataagacat
    21121 ctcttactcc acaagccaat aaacctagtg aatataatct aagcgctata gatgtaaggt
    21181 ctgttgataa cgcatcaaat ttaccccttt ggaataatat tctaacaaga ggctctgcta
    21241 atataatagc tcctatagat ataggaagca ccaccaatat aatacaattt gaagatttta
    21301 caatagtgct tataaatcct tctttattat ctttaccaga catttttgct agtttaggat
    21361 atataactgt tattatagat gtaacaaata atgccataac aaactcattt agcctatttg
    21421 catagttaag agcagacaac tttccatcac ccaaagtggt tgccaataat cggtcaacaa
    21481 atatatttaa ctgtccaaca gctactccta ccaacatagg caatactagg ttgactaact
    21541 cttttataga ctcatccttt aaatttatat atggcttata cttgtaggac ttcttaaacg
    21601 caaatggcag ttgaaacaat agctgactcg ccatagccaa taatgctcct gctggcagta
    21661 tccatacatt tttacctgca cttagtgcta ttgctgctat tattataata ttatatggga
    21721 ttcctataag cccaggaatt acaaaactgt cattcacctg taaaaacgaa ctaaatatct
    21781 tacttccact caggaataaa actcctgaaa tcattatttt agtaaataat attgctgctt
    21841 ggaacttagc agggtcgttt ctaaatcctg ctgcaaatac tgtaacaaat tgctctgtaa
    21901 ataaaagccc aataatagct attactatag ccattatata gcatatattt aaaacattat
    21961 tagtaaattt taaggcacct tcatctccga gtcttttctt tgtctcaaag tacataggta
    22021 tataagttgt agcaactgca gagcctatga ctgctatgat taaaccaggt atatttaatg
    22081 ttaatacaaa tacgtctgca tacatacctg tcccatagaa atttgcaagt acaagttctc
    22141 taaggaaacc taatactttt gaaaacatag tggctgccat aatccacaat gctgctttag
    22201 ctgtttttga catcttcttt ctccttatct aatttaaact ctaaaagtag aacttttaaa
    22261 actgcttata aacattattc taattaagta aatttaacct atgaaaagct tccttataaa
    22321 tcaaaaacta tttcgactca tatctaagtg ccctaaatgt aatcaatagt aataatagta
    22381 ttaatactat acaagataag aatgttagat taaatatgct cttaaataga ctataatcac
    22441 ctactgtata cccaacttca actacatcat ttatattgct atctttcaag aatttatctt
    22501 gtgcacgact taagttatca ctacaaatca gtattggcat attttctttt gctgatacta
    22561 ctcccaaaga aatatattcg tttacatcag atctactacc aaagtctaat ctagcagtat
    22621 agattttctt tatatctttt tcatcataaa acttctttat taaatcaaca tttgtacttg
    22681 acttatttat ttgctttatt ccttctatat tttttaagtt agaatcaatc tctgcaataa
    22741 actcttttga atcacctaat gcatatattt ttttaagatt cttgcctttt aagaaatcaa
    22801 tttgttcctc aaacccttca tctttagccc atattattgg cattccactt ttagcggcat
    22861 aagaatatac agatatggcg ttctctaatc ctgttgttga gctaagtaaa aatgcttcat
    22921 caaatcctac tgttctatac atttctctag ctatctttaa agatgtatca tatctgtctt
    22981 tccctcttat tctttctact ttcattccca ttttttctat attacgttca gcatctttag
    23041 atactgcctt taaaccacct actatcgtaa ccttctctgg acctagttct tttagataat
    23101 ttcttgttac tctaccaaga tttttactct tagttacaac tattggagca ttttttgcat
    23161 atgctaaagg tgttgcactt atactatcta caattgactc ttcatttaca agaattactt
    23221 ctttagaatc cttaaatgct cttttattta attctgtcga aatctctggt atactccctt
    23281 ctatcaactc tcttttggga tttgcatctg caaatactcc tatcgtacta gaaatagaga
    23341 ttaacaaaac taacgataat atagtagaaa tttttttaat cataagttct cattcctttt
    23401 cttttttgta ttttttgaca ataaaaaagc tctttttaaa gagctttttc attgattagt
    23461 aatctaatat tttaaaatta tctagctccg tctcctgtca atactacttt aacagtttta
    23521 aagagtatct taaagtcaag taatatactc ctatttttta tatagtaaat atcttcttta
    23581 agtttttctt caggagtgat ttcgtatcca ccatttactt gtgcccaacc tgtaagccca
    23641 ggttttatag cgagtctgtt gataaatcca ggtatttcct cattaaattg aacagtaaag
    23701 ttcggtctct ctggtctagg acctattaag cccatgtccc cctttaatat attaaacagc
    23761 tgtggaatct catcaattct agttttccta ataaactttc caaccttagt tattctagga
    23821 tcatcttttt cagcccattg tgcgccaaat ttttctgcgt ctgttctcat agatctcaat
    23881 ttatatatat agaatcttct cccgcatttt cctagtcttt cttgcttata agttattggt
    23941 cctttgtcct ctattttaat aagaattcca aatattgcta ttaaaggtaa acctatcaca
    24001 agtcctatta tagataaaac taaatccata attctttggt acaagtcgaa taaaactgtt
    24061 ccttttacta tgctatagtc aatctcactt gcaatttctt tatctgcccc tggatagctt
    24121 ttaattttag gtatttcaca ctcatcatta gtatatccag acatagttac ctccatattg
    24181 gaataaaaat ttttttaatt cttaatttca acttatttct atcttatttt tgacttattt
    24241 ctatattata ctcaataatg agcagattat caatacaaga aacatcattg taaacaattt
    24301 gattctttac aaaaatatat catctcttgt tatattttac attataacag gagatgatat
    24361 gttttgcttt aatattttct attttatttt actgtatctt ctattcctat tccaatttga
    24421 gttcccattg tttttacttt ttctgcagta tttaacttat ccatatcgct cttattgtca
    24481 ataaatgcac attcaacaag tattgctgga taattattat ttcttaacac atataggtaa
    24541 tcttttccat tatcaagtgt tcttgtcttg attcctctat ttttcatgtt aaatttttct
    24601 agtattcttt ttaagatatt tgatgctgca gtcttagttg ttccaccatt tttatctttt
    24661 actttgtaat aaatttccac accattacca gctccattag acgcattgta atgtatactt
    24721 gtaaataaat ctggctttat agtatttgat aatgctgttc tttctcctaa agccattgtt
    24781 ttatctgtat ctctagtcat aacaacattt ataccttttg aacgtaaata ttcagtagtt
    24841 gctaatgctg tattaagagt atatttcttt tcttgtgctc caccatttaa tccacttgtt
    24901 gcccctgaat cagaaccccc atgacctggg tctatcacta cagtttttcc tgctgctgaa
    24961 cctgaattat ctggttctgt tggtgcattg tgttttgata aactagatgc tatagaattt
    25021 ataacactat ctttcattcc atcaccaatt tgatatactg ttcctgctgt tttttctgat
    25081 aaatttgtat tgtggtgtgc agaaacataa gtctttggag ttataagtat tggagctttt
    25141 aatttagctg ctaaaggtcc tgctgttatt gaatcaacta ttatgtcaga ttttgcaact
    25201 aacattgcac tcaactcttt atctggatag aaagtcttaa ttacatttgc atttgtttca
    25261 tgtctatctg ctccagaaac tctatttttt gaagtaccat tttttgctat tttagatatt
    25321 tggtctataa gttttgatga taaactttga ctaccaccta tgtaataagc ttcttctaaa
    25381 ccttgtgaag ataaccaatt atatgtacct tgtggtacag aatctttatt agctaatatt
    25441 ataggttgtt ggtcttcacc agcttttgat gaaatattaa gagcatcata ttcaccagca
    25501 tatccatttg ctatgtatat tttattaaca tcatgatact tatctatttc ttttgctata
    25561 agtaaagatg tttcatgtct atcaactcca ccaatacgag tcacatttac atttacagca
    25621 gatttaattt gagacactgc cttttgtgaa actgaaccat catcacctat aattattaca
    25681 tcacttggat ttagacgttt taactctgct tttgtacttt ctggtatatc atctttattt
    25741 gcaagtaata ttggtgcgtc atatgtagat gcaagtggag tagctgtaat tccatctgct
    25801 gctaattctc cattaactat tacaacctta ctagacccat ctttccaacc ctgtttactt
    25861 atctcaacag cagtttcaaa tctagtagct ccagcaagtt tttttactgg tgcagaagtt
    25921 tttctcatat attcatttac tgcttttgtt gaatttgcat cacttattat aaactcttgt
    25981 gtaacatcat aatccttaag atttaatccc atagactcta agtcttcttt gtttaaaact
    26041 ttctgttcat ctttaccatt tatttttatg ctttgaagtt cccatacatt taagtattct
    26101 gggtctttgt tatagtttat tacaccttca tatgaatctg tcttttcatt gtaatttaaa
    26161 gtagcttcaa gtggcatatc ataacacata aaatttaatg ttattgaatc agcatttaat
    26221 ttttctttaa attttacact tgcattagat ttacttccta acactacact ttttctttgt
    26281 aaatttatat taatatctga attagattct gtatttgcag tagtattttc agccgctaaa
    26341 gctacgtttg gtgccaccat tgctacagac aacattcctg ttgccaataa ttttgttgtt
    26401 tttttcatca tatcccccct aattttttgg ttctttaact ctttaaatct tttttaatac
    26461 atcttaagct tgttttatat tacataaatt caatattatg taaatctttt ttcattatag
    26521 cacaaaaaat tacaaattgt aaactttttg taatctttta tggcatggtt tttaaatttt
    26581 atcgaggaga aattttgatt atatttgata gtttccgtat atttgaacaa atcaaagact
    26641 ttatcatcta aaaatattgc actaaatttt aataaatatt tttttcttgt acattataga
    26701 cattactata acccagaata ggacatacaa taaagaaaat ataagagaat cccaagttgt
    26761 acctgcccaa ggtgttataa gttcatatgt agtccactct accaaggtag ttggataatc
    26821 gactttgttc gtaagttttg gaacattcca aaaaacatga cttaatattt caaggcacat
    26881 atatgtaaaa atagggctac ttccaagtgc tattattggg gtaaatattt tacttttatt
    26941 tttgatgtcg catataaaat aaaatattga cagtaatata ccataactgc cagccattaa
    27001 aagtacaaat gagctagacc ataatctttt attaaatgga aaatactgat taaagataaa
    27061 agcacctatt agcaggatta tgctcataac cagtatttta aagaattttt tatattctcc
    27121 tatgtcttct tttaaaagta cacatcccat agtgcatccc aacatacctg aagaaattgc
    27181 tactatacta gttaaaaaac catctggttc aaattccggc ttgtacaaat gtcctttgaa
    27241 gaaatgtaaa tccactaact gtgcaagaga accttctagc tcaaatccat aaggttttgc
    27301 taatatgtag tacccaacta ttataaatgt agatatacat aagaaagtaa ttattgtagc
    27361 agtatttcca acatttaatt tttttagcag taaatatact aagctagtca caaaatagac
    27421 taatcccata cgttgaagta ctccaagtat tcttacagtg tctaaatcag gatttcctag
    27481 ataatttaag aaaaatccaa ataatatcaa taagatactt ctcttgaata tactaagtat
    27541 tatactcagt gtggatttat tattttttat cttactattt attgatattg gaattgtaac
    27601 acctaaagat ataacaaaaa aaggaaatgc aaaatctgca agtgttacac catgccatac
    27661 agcatgtctt aattgaggat acattctcat ccaagtccca ggattattgc agactatcat
    27721 gagggctatt gatagccctc ttattatatc tattgattta atcctactat tcataagttt
    27781 agaatctaca gcattatttg aaatt
//
LOCUS       DAEORZ010000042        26871 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEORZ010000042 DAEORZ010000000
VERSION     DAEORZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26871
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.042"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(300..806)
                     /locus_tag="KRH39_001630"
     CDS             complement(300..806)
                     /locus_tag="KRH39_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBG8511543.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTIAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            complement(1162..2190)
                     /locus_tag="KRH39_001631"
     CDS             complement(1162..2190)
                     /locus_tag="KRH39_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG8511544.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEYEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            complement(2238..3098)
                     /locus_tag="KRH39_001632"
     CDS             complement(2238..3098)
                     /locus_tag="KRH39_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HBG8511545.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDIEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            3367..3576
                     /locus_tag="KRH39_001633"
     CDS             3367..3576
                     /locus_tag="KRH39_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG8511546.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            3576..3842
                     /locus_tag="KRH39_001634"
     CDS             3576..3842
                     /locus_tag="KRH39_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG8511547.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            3871..4443
                     /locus_tag="KRH39_001635"
     CDS             3871..4443
                     /locus_tag="KRH39_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG8511548.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(4677..5867)
                     /locus_tag="KRH39_001636"
     CDS             complement(4677..5867)
                     /locus_tag="KRH39_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG8511549.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(6127..6558)
                     /gene="dut"
                     /locus_tag="KRH39_001637"
     CDS             complement(6127..6558)
                     /gene="dut"
                     /locus_tag="KRH39_001637"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBG8511550.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            6671..8899
                     /locus_tag="KRH39_001638"
     CDS             6671..8899
                     /locus_tag="KRH39_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBG8511551.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(9010..9495)
                     /locus_tag="KRH39_001639"
     CDS             complement(9010..9495)
                     /locus_tag="KRH39_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8511552.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            9747..10970
                     /locus_tag="KRH39_001640"
     CDS             9747..10970
                     /locus_tag="KRH39_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG8511553.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            10974..14108
                     /locus_tag="KRH39_001641"
     CDS             10974..14108
                     /locus_tag="KRH39_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBG8511554.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            14142..15440
                     /locus_tag="KRH39_001642"
     CDS             14142..15440
                     /locus_tag="KRH39_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBG8511555.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQEEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(15729..15974)
                     /locus_tag="KRH39_001643"
     CDS             complement(15729..15974)
                     /locus_tag="KRH39_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG8511556.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(16188..18815)
                     /locus_tag="KRH39_001644"
     CDS             complement(16188..18815)
                     /locus_tag="KRH39_001644"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG8511557.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVVVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(18876..19718)
                     /locus_tag="KRH39_001645"
     CDS             complement(18876..19718)
                     /locus_tag="KRH39_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBG8511558.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(19725..20363)
                     /locus_tag="KRH39_001646"
     CDS             complement(19725..20363)
                     /locus_tag="KRH39_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8511559.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(20799..22091)
                     /locus_tag="KRH39_001647"
     CDS             complement(20799..22091)
                     /locus_tag="KRH39_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG8511560.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(22128..22484)
                     /locus_tag="KRH39_001648"
     CDS             complement(22128..22484)
                     /locus_tag="KRH39_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG8511561.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(22519..22878)
                     /locus_tag="KRH39_001649"
     CDS             complement(22519..22878)
                     /locus_tag="KRH39_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511562.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(22947..24920)
                     /locus_tag="KRH39_001650"
     CDS             complement(22947..24920)
                     /locus_tag="KRH39_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG8511563.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSINEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            complement(25142..26155)
                     /locus_tag="KRH39_001651"
     CDS             complement(25142..26155)
                     /locus_tag="KRH39_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG8511564.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            complement(26168..26713)
                     /locus_tag="KRH39_001652"
     CDS             complement(26168..26713)
                     /locus_tag="KRH39_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG8511565.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
BASE COUNT         9216 a         4437 c         3370 g         9848 t
ORIGIN      
        1 tctatattaa aataaatcaa gtacaaatgc cataaattaa gtgtttctag ttagcataaa
       61 aatacttata aagtatactc aagtatttca aataaatact tttattcaaa taaatctcta
      121 cttatataag tgcttatata aataaaaaga tgaactatag taaatatagt tcatcttttt
      181 tataatagta tttatttaaa attaatattt atttagttac tagcatctac ttagatatca
      241 gataagaaag cacagtcatt tacttataat aagttctttt catatatttt ttattataat
      301 tattctttta caagaacttt tataatttct gaaatgtaca tagtatggta gtctttctct
      361 ggataccatt ttttatcatt ttctttagca tcaaagtttt ctggtggcat attaccatga
      421 tatagctttt tacatactat aatcatatta gcttcttcaa aagctaccgt atcatccaca
      481 aaatatggtg taagtcctgc tttttctatt ttatttatat ctctaccaga aatagtgcca
      541 caaatattta aagcttctct atacgtttca tcaaagaagg caattgtaaa tgtatcatta
      601 ctatctacaa attcttttgt atatctctgt ggtcttatgt aagttgtaac aacattttta
      661 ccccaataga ctccaacacc accccagcta gcagtcattg tgttaaactt ttctgaatca
      721 ccagctgtta taagtaacca ttctttgcca atcttagtaa atggattaaa ctgtaattcc
      781 tcaattttta cttctctaaa acccatgact tcatcctacc cttcattttt taattatata
      841 aattaattgt taaaaatttg aatatatgta ctcttataat actgactttt gattgtccta
      901 cataattcat gtaaattata atacaacaaa tcaattactt tcgcaataag tagttttcat
      961 attttataag tgtaaaatat agataaaata tattaattat aaagtatata atatattttt
     1021 aaacctaaag caatatacaa taaaaaaatg tagaaagtaa gtatctctct acattttttt
     1081 attgtatatt atttttatat taattcagtt ttataaaaaa ttgttataat tgtcaagttc
     1141 tactctatta tttataattt cttatatctt attaacaaca tttaagttat tacatttatt
     1201 tatatatatt ttttcattat ctaaatcttc atcaaacatt tttattatat tttttatctc
     1261 atacttgtac tcatattcat tcattctata aagcattaaa tcttctagta catctttcat
     1321 gtcgaagtac acatcttcta ataaataaaa atagccttcc tctatatctt tcaatttatc
     1381 tatagtatcc atttttttac ttatgtcttt cataatttct aaattttgta tatttacatc
     1441 tatgattgtt aataagtaca ttctcacagt ttttattatt tcaacagaaa aatctacaat
     1501 attataattt ccttcttttt tatataatga ctccttaaat ttgttaaaac aattatctaa
     1561 attagaattt atatcgttaa atggtggtct tattatgtca cgtattatta aaagattttg
     1621 tatgtctaca ttatttgtga ttgcctcatc caaaataaat aaattatatg catcatttaa
     1681 tgggtcatgg gcatctccac taaactctaa gttaacaaat tcaagtaaat ttttcaatga
     1741 tatatagttc atacatctca taccacaatt tatatatttt tcttttatat ctacaaataa
     1801 atctctaatg ttatttacaa accttggatg gttatttttt ttagcacttc tcatatctgt
     1861 gttattgaaa catgtcaaat ccaaattacc aaaagtatat attcctttaa tatcagaaaa
     1921 tatgccaagc cattttttaa actttctcat gacctcttca taacttggtg cactcttcaa
     1981 ttcttcagaa gatatatgtg ttaattcttg tatatgagga tacaactcct cattacttac
     2041 tggtcttatt aaagatttaa atttatcaat attttttgtt ttttcatcat acataactgc
     2101 tccgattgca attatatcag aattaaatac tgctcttttt gatttagaat ttggcatatt
     2161 catttcaaaa tctatatata ctcttttcaa tataaacacc ctttccaatt gtttatagtg
     2221 attcttttta actattttta ttcaaaattt tctaagaaag aatgtttctc cccatctttt
     2281 tgccaaccaa gtactgaatc aagatttaca ttttctgccg ccttaaatat agatttttct
     2341 acatttttaa aatttgattc taaactcttt attaagtctt ttatctcata tcttttatta
     2401 cctgtttttt tagcagcaac catacatgca caatttaatg gccatatacc actattttga
     2461 ataaatctta taatatgttc ttctcttata taataaagtg gtcttataat ttttatacct
     2521 tcaaaattag tagaattaag tttaggaagc attgttttaa aatttccagc acacaataag
     2581 ttaagcattg ttgtctctat tacatcgtca taatgatgac ctaatgcaag tttgttacac
     2641 cctaattctt ctgcctttga atataatgct cctctcctca ttcttgcaca catatagcat
     2701 ggataatctt ttgctatttc atctgctatc tcaaatatcc ttgaatcaaa taaatgtatt
     2761 ggtatattta gatactcaca attatctata agtaattgtc ttatatttgc atgatatcca
     2821 gggtccatag caataaattc aatatcaaaa ttaacttgtc catgtttttt taactcttga
     2881 aacattttag ccataagaat actgtctttt ccaccagaaa tcgctacagc tatcttatca
     2941 ccttcttcaa ccaatttata atctcttatt gccttcataa acttagacca taaatttttt
     3001 ctatattttt ttattatact tttttctata tctcttactg gaagtctttc ctcaaaagga
     3061 actataattt cacagccttt tcctgatagt tcactcatct aatcacctcg tatatttatg
     3121 ttaataatat tttacaaaaa agtctacctt catccacaaa atactataat ttttacttta
     3181 attttactaa ataatttata gtttacaata acacataaac ttataataac ataaataacc
     3241 tctttctttt attatctttt ttatttttct catttaatat tttattatta gtaacgattt
     3301 ttaaatgatt gaatattata cactagatag agttacctat ctatttacaa caaggaggtt
     3361 ttcatcttgg aaaataagac tagagatatc aaaaaaaatt gtggtccatc tttggctaga
     3421 ccatacatta ataatcaaat tttcacagaa atgtactgcc tttctgatgc attaaaattt
     3481 ggaactatat ttccagaact aaacttacta gagtctgaaa tgtacaacaa agaattatat
     3541 gctaagccaa aaaaatatag aggaggtaga agataatgaa agactcatct agagctgaac
     3601 ttatgagaaa agttcaggaa acatcttttg catgtgttga tatgaactta tatttagaca
     3661 atcaccctga tgataaaaat gctatcaaca cttataattc attatgtaat caatttgcgc
     3721 aagctagata tgcttacgaa aataaatacg gtcctttaac taactttggg tatgctccaa
     3781 gtagatgccc ttggcaatgg gctgaccaac cttggccttg ggacagagaa tttaattatt
     3841 agacatcaag tattggagga ttgaaaattc atgtggatat atcagaaaac tatacaacac
     3901 ccagttaata taaaaacttg tgaccctaga atggctaaat ttcttataac tcaatttggt
     3961 gggccaaatg gagaacttgc tgcatcttta agatatttaa gccaaagata tacaatgcct
     4021 actggtaata tgcgtgcact tttaacagat attggtacag aagaactagc tcacgttgag
     4081 cttatatgta ctatggttta tcagttaact tctgatgcaa gcccagaaga gttaaaagct
     4141 gcaggtcttg gttcaaacta tgctcaaaat ggatatggaa tttatccaac agattcaaat
     4201 ggtgttccat ttgatgtaag acctatagca gttatgtcaa atcccgtaac cgatttacat
     4261 gaggatatgg cagctgaaca aaaagcactt gcaacttatt atcaacttat aaacctaaca
     4321 gatgacgttg atgttataga tgtattaaaa ttcttaggtc aaagagaaat aattcactat
     4381 caaagatttg gtgaagcttt aatggatgct tacgagttag aagaatctca aaaaatgttc
     4441 taaaattttt atttgtataa ctattattat ttgataattt ataatttact gttgtgttaa
     4501 aatttataaa aaatataaat ataatttatt ttctataaac agagaaaata aataaaaagc
     4561 aggtatcaat aaaatagtaa catgtattat actatacatt actatcaata ttgtacctgc
     4621 tttcatatta taagcaataa agaaaaatct atttattagg ttttataata aaacaattat
     4681 aaaactcttc ttgcatttac atatctactt gaatagtagc tactatttat attagatatt
     4741 gttacttttt ttgatgaacc tgaagatgca tgtatcatat ctccatttcc tatgtatatt
     4801 ccaacatgac ttacagaacc attatttgaa ccttgtgtat caaagaatac taaatctcca
     4861 cttctaaggt ctcctctatt tacatatgtt ccatacttac tttggtctct agatactctt
     4921 ggtatactaa ctcctacaga ttttttcata acgtactgtg tgaatccaga acagtcaaaa
     4981 ctatttggac cttctgctcc ccatacatat ggttttccaa gtagtgtctt tgcaaaactt
     5041 attacactat cagctccttg acttggagaa ttagtcgaat aagatggaat tgaagtatca
     5101 acagaacctt tttcagcaag gtattgactt gacacatatc cagtttgatt gccataactt
     5161 acttttgtcc atcctccact ttcagaaata cttccaacct cagtaccata acttaatgta
     5221 gctattttag aatgacttgt acctggtcct gttctaacgt tcaatgactt tgctgttaca
     5281 acctttgtac cctttacaag gtcatttgaa ctgttttcag cattttcatt tgtagagtca
     5341 tttacactag ttcctaaata tttacttgaa acataaccta ctctaccatt agaacttatt
     5401 tttgaccatc catttgactc agatatgtat ccgacttcag tcccatatcc tagtgttgat
     5461 attttagaac tacttgtact tggtcctgtt ctaaaattta atccttttgc tgttacaact
     5521 tttgtcgact taactgaagt atcactactt tcattgctac ttccaaggct atttattgct
     5581 acataatttc catgcacata acctgtgttt ccattatatt taacttttac ccaagaacct
     5641 tcttttccta cgtactcaac tttatcacct ttgtaaagtt tccccattga ttcatggttt
     5701 gtacctggtc cttttctaaa atttaccgca ttccctgtta ttgttctgct ttctatactc
     5761 acactctcta cttcagactc attagcatat gctaaattcc ccatagtagg cattattatc
     5821 gcagttgcca ttatactggc tgctattctc ttttgattaa aattcataac tttccactca
     5881 tctcctcttt tctactatct ttatttcact tcaaaatata aaagaaataa ttgacactat
     5941 caaaatatca attatttctt ttatattaat attattacaa atttgtaact atttgtctat
     6001 agtaatattt gcttaccata aattaccatg tataaatttt aacctttaat agcctgcaat
     6061 ttagtaatat agccatcttt tacctttttt aaaaatggct atatttttta acttttttgt
     6121 aactttttaa aatccagttg agccaaaccc accttttcct ctttcggagt cacttaattc
     6181 ttttacctct tctatgttaa tatcatagat aggttttact atcatttgag caattttcat
     6241 atgcttttca accttaaaat catttttacc atgatttatt aatataattt taagctctcc
     6301 tctgtatcct tcgtcaatag ttcctggtgt atttaataca gtaacagaat gctttaaggc
     6361 aagcccactt ctaggtctta cctgcgcttc tgtcatagta ggaagctcta tacatatccc
     6421 agttttgata agtttagtct ctccaggagg tattacaacc tcttctatag aatacaaatc
     6481 cataccagca tcacctttgt gagcaaaatt aggtattata gcatcatcat ttaatttttt
     6541 tacttttaaa ttgtacatct tatatctcct ttcaactttc tataaatatt ataacttaaa
     6601 tttcgacctc ttgaagtttt ttattttttt gtggtaatat aattattagt gttttaaagg
     6661 aggatttttt atgaaacttg tacttgctga gaaaccatct gtggcaaaaa ctatagcatc
     6721 atttttaggt gcaaagacca gacaagatgg ttactttgaa ggaaatgatt atataattac
     6781 ttatgcagtt ggacacttag tatcattata tgatatgaag gattatgata aagataaata
     6841 ctctggctct tggaaaatga acaatttccc atttgttcct gaggataagt ttaaatttaa
     6901 gattgatagt tctaaaacta aacaattcaa cactgtaaaa aagcttttga atagaaaaga
     6961 tgtagaatat gtaataaatg ccactgacaa tgaccgagaa ggagaactta tagcattttt
     7021 aatattttta ctggcaaaaa ataaaaaacc agtaaaaaga atcttggtaa atgaatggac
     7081 accagaagat attacaagag gtataaaaaa cctaaaagat gaagatgaaa tgagaaatct
     7141 tcaagctgct ggatatacta ggcttattac tgactggttg attggaataa atttcacttc
     7201 tgttgctaca ttgaaatatg gaaatgggaa actattaaac ataggtaggg taattcttcc
     7261 aacagttaaa ttagtctatg atagagatat ggaaatacta aactttgttc ctaaaactta
     7321 ttatgaaata gaagggcatt ttaaagctga agctggtgaa tataaaggta aatatgttaa
     7381 gggtaaagaa agtaaatttg atacacttga agatgcaaat aaaattatag cttcaattac
     7441 atctgaaact ggtaaaatac tagataaaaa agttactatg tcaaaggaat atgcaccaaa
     7501 actttttagt ttgacatcac ttcaaggata tataacttct aagtattcca attttacatc
     7561 tgataaggtt ttaaatgtct gtcaatctct ctatgagggt aatggtaagg gtggatacat
     7621 aacttaccct agaactgatt ccattttctt agaagaaagt ttggcttcaa aagcttctca
     7681 aactttagat aaattaaagg ttggtcttga atatgaaaat aaaattaaat tttctaaaac
     7741 taaaagagtt tttgattctt ctaaagtaga ctcccatagt gctataattc caacttatat
     7801 aattccaaaa agtttatcta aggatgaaca attagtttat gatgccataa aagatagatt
     7861 tgtagcaaac tttatgcctc ctgctgaata tgagaataca gaaattaaga ctgaagtaga
     7921 taattgtact ttcttaacaa aaggtaaggt gcttaaatca aaaggatacc ttgaggtata
     7981 taataaggaa gaaaaaaacg atttacttcc acttgtaaat aaagatgatg ttgtagatgt
     8041 gttagaaata aaaccattaa ctaaacaaac tactccacct aaaccatata ctgaggacac
     8101 acttttaaag gctatgaaaa attgtggtaa aaatgtgcct gaagaagata caacagtgct
     8161 atctggatat tcgattggaa catcagccac acgtgctgat gtgttaaaga agataagtca
     8221 agtgggctat gtaaagaaaa aaggtaaatc ttattctata acagaccttg ggaaaaattt
     8281 agttgaaatt tttcctgtta aagacttatt tgatgtagat tatactggaa aacttgaaaa
     8341 aagtttaagc gatattcaaa aaggaaagta tacaagaaaa gaatatctta ctaatatcat
     8401 tagttttata tatcaaaatg tcaatattat aaaacaagat gcttcaaaaa atataaacac
     8461 agaagcatat actttcgatt caaaaactaa aaaatttact aaaacaaaag aaaaaaaaca
     8521 gaaaaaagat gtaactaata ctgaaaaaga agttgctata tcaaaggatt ctaataacaa
     8581 aaaagaagtt aaaaatggtg atgaaaatca atctcttggc aaatgcccca tttgtcaagg
     8641 tgatgtacta gaatttgaca aaggctttgc ttgtaaaaat cataaagaat gtaaatttgt
     8701 tatttggaaa aatgataagt atttaagtct ttataaaaag aaagttaaca aaacaatggt
     8761 caaaaatatt ttaaaaaaag gtgaaactaa agtaaaatct ttaactgcta aaaacggtaa
     8821 taaatttgat gctacattaa aatatgataa aaatccagat actggctatt tcaattggaa
     8881 aatagagttt gacaactaaa tattcataag aaaagaaata gcagtagtat aataatctat
     8941 attatattac tgctatttct tttcttatga agttttaact tttttaatag tgacttttac
     9001 aatcacactc taacctaatt tctttactat ttctgataac tttaatgtat gttcatctaa
     9061 taaagttagt tcctcttcag aaagattaga taattttttt actagagatt ctttgagaat
     9121 agcttttact gcatcaaata atttaaatgc tttatctgtt aattcaaccc taagaatcct
     9181 tctatctttt gggtcaggaa atttattgac taaaccatgt tccactaatc tgtcaataat
     9241 cggagtcata tttgatttta aaattcctaa attagaagct atttgagaaa ttgatgatga
     9301 atttgatgtc ataagataaa gaattacctt agcatgtgat ggagccattg gacattcttc
     9361 aaaataagct ttcattatat cagagttatt ttgaaacttt tttaacaaat cgctttcttt
     9421 aaatatatta ttttcaagca caaataaaaa atctaaaaaa gagtccgcta ttttaagtat
     9481 attgttgtta tccatatttg taacatccct tcaatagata atataataaa atattgttgt
     9541 ttaatatagt ataaattata aactattaat aaatttaaaa caagaaaaag ttattgactc
     9601 ataataatta taaatttata atcaaattag agaattataa attgccaata aagggaagtt
     9661 ttttaattag tctaaaacta aacttaaatt ataagacact ttaaattcta caacaagcta
     9721 gtgtgcaata tataatagga ggagatatga aaagatattt aatagttttc ttaatgtgta
     9781 gttcactgtt tttagttgga tgtggaaaaa cagaagaatc tgaacctgag aaaccaatag
     9841 cagtttctgt tcaaaaagca ataggtggag aaattgaaaa cacaaattct ttctcaggaa
     9901 ctacaaaggt taaagatgaa acagctgtta ctgcacagac tgttggcaca gttcaagaag
     9961 tgtatgtaaa acttgggcaa aatgtaagaa aaggagatga attattgagt ataagcagtc
    10021 cagagctaga aaatagtgta aagcaaagta aagcctcact agatttagca aaagcaagtt
    10081 attcaagtgc cactggtggt tcattagagg cacaagtaaa tcaggcaaaa acagctcttg
    10141 acaatgcaaa gattcaatat gaggagtctc aaagaaatta tgacaataat aagattcttt
    10201 atgaacagga agtcattagt ttagaccaat ttaaaaaaat agagttttct ttagagcaaa
    10261 caaaacaaca gttagattct gctcaaagag catacgatac agcaacttca aaaagtatac
    10321 ctcaagctaa agcacttgct aaaaaacagc ttgaccaagc tcaagtatca tataatttag
    10381 caatgagtaa tttagacaaa ttaacattaa catctccagt agatggtaca ataacagcta
    10441 aaaactttga ttcaaaggaa atgataacac aatcacaacc agcttttatt atttctaatc
    10501 ctaatatact agaagttgat ttaaatgtag cagaatcaga tataggtaaa tttaagaaag
    10561 atggaaatgt agatgttata attgaagacc aaaggatttt aggaaaaata gattatgtac
    10621 cttcagtagt tgaccctcag acatctttat atcctgttaa agtacttgta aataatacaa
    10681 acaacaaatt taaggcaggt atgtctgctc aagtaaactt aagtgttgaa aaagaaaatg
    10741 gtgctgttac agtacctaaa aaagcaatat ttgaagaaaa tggtaaaaaa tatgtataca
    10801 tagcaacaaa agacaatata gcaaagaaac acttggttca aactggaatt gtaacagagg
    10861 ataaaataga aattaaaagt ggtgtaagtg ataaagatac agtgattatt ggagggataa
    10921 gtcttatttc tgacggaact aagatatttc ctgtagaaaa ggagaagtag actatgaatt
    10981 taacacagac ttccgttaaa cgtcctctca caatcataat ggtcttttta gtattaatag
    11041 tttttggtgg tattggatat aaaaaaatgt caataaatct aatgcctgat atagaaatac
    11101 cagttgtaat ggtcatgact acttggacag gtgctggtcc tcaagacgtt gatgaacaag
    11161 ttagtcaaaa agttgatgaa agtttatctg ccgtttcaaa tgtaaaatct actatatcaa
    11221 gttcacaaga aagtgtttct atggtagttg ctcagtttga atttggaact aatttggatg
    11281 aaataatgaa tgatgttagg tctaaagttg atgcattaca gaccagcctc ccagatgatg
    11341 cagctaaacc aacagtttta aagctggata tgaatgcaca ggctataggt caacttgtca
    11401 tatctggagg aaatgaaaat agttcacaag ccttaagaaa atacgctgaa gatgtaattc
    11461 agcctaaaat agaatccata gatggtgtta cttcagcaga ccttaaaggt ggagaaaaag
    11521 cacaggttaa tgtaatagct gacccagctg ttctttcaaa ttatggagtt agtttatcca
    11581 ctataaaagg tgtcttatcc tctagtaata aaacattccc ttatggttca attactcaag
    11641 gtgaggataa aatagtttta agagcgattg ataaattaga atccctagat gatataaaac
    11701 agattcaaat tcctgtaaaa ggaggaaata cagttagatt agataatctt tgtagtgtag
    11761 attatggata tgcagacaaa gatagtattt atagatacaa tggaaaagac agtttggtaa
    11821 tagatatttc taaacaacaa gatgccaaca ctgtaaaagt tatgcagagt gcatacaaat
    11881 acgtagatga attgaataag gaaaatcctc aatttaaaat taaaattgct aatgatacaa
    11941 gttcatacat aagtgaatca ataaacagtg taatgagcaa cttgcttata agtgctgtta
    12001 tcgcatttat tgtaatattt gctttcctaa aaagtgtaag agcttcacta gttgttgcag
    12061 ttgcaatacc aatttctata ataggtgcta tagcaattct ctactttact ggtgaaagtt
    12121 taaatcttat tactgcaagt tcattggtaa tttcagttgg tatggttgtt gataatgcta
    12181 cagttgtaat tgaaaatata tttaaatata ggaaaagtgg tagattgagt cttgatgatt
    12241 gtgctattga gggaacaaga actgtaacaa atgcaatact tgcatcaaca ttgaccacaa
    12301 tagcaatatt cttacctatc atgtttacag aaggtatagc taaaataact tttggggcat
    12361 taggtaaaac actaatagct tcactagcat tctcctttat aggtgcaatt acactcgttc
    12421 ctagtgtatt tgcaaaatta agcagaggaa aaaatagtca aaagatgcaa gaaaaagata
    12481 gtcctatatt tgataaggta agtgaagcat ataaaaaatt attaagtgta tgtttgagac
    12541 atcaacgctt agttgtaata tcaagtttac ttatttttgt agcttcctta tttggaatgg
    12601 gatttatagg tatggacttt atgcctgcag cagacaaggg tgaattatct atttctatag
    12661 aacttccaaa aggtcttagc ctagaatcca atgattacta tgtatctatg actgagaaaa
    12721 aagtctcaga tattccagaa attaaaactt taataacaac tttgacatca gattcaaata
    12781 atagtaaagc aagtatagca attgaattag aatctcaaaa aaatagaaaa agaactactg
    12841 atgaaataga aaaagagata attgatagag tggctactgt accagattgt aaaataaatg
    12901 taagtcaaaa cagtagtgtt atgggtggag ctggaggagc agacttcaca ttagaaatga
    12961 aaggtcctga tttagatgta ttaaaaatac tgggaaaaga agctgaaaaa gaatttagct
    13021 ctataaaagg atttagaaat gttgaaactt ctcttacaga tacaacacaa gaagctcaat
    13081 ttactataga taaacataag gctcaagaat atggtgtaaa taccgctgaa attgcaggca
    13141 tgttacgtac agcaataaat ggtgactccg taaccactgc tactattgat gattacaaag
    13201 tggatgtaaa cctaaaattt aaagcagata ctattgatag cttagaagat ataaagcaac
    13261 taaaagtaaa ctctgcaact ggaaaagaag ttcctcttgg tgccttttca gatataaaaa
    13321 tggcagatgg tctacaatct atatctagga ctgatggaga tttttctgta aatataactg
    13381 ctacacttga caatttagat gctggtactg ctacaaaact agcactagaa aaaacgaata
    13441 aaatggtaat tcctaaagat tactctatag gtgttggtgg tgaaacagaa atgatgaatg
    13501 aatctatggc aagtcttgga ttttcaatgg tcatagctat agcattagtt tatatggtaa
    13561 tggtagctca gtttgaatca tttagtaaac catttataat catgttctgt gttccatttg
    13621 cattcgttgg agttgtgctt tcgcttctag ttagtggtca aagtttaagt actgtcggta
    13681 tgctaggtat aatcttactt atcggtattg ttgttaataa tggtatagtt cttattgact
    13741 acatagagca acttagaaaa acaaatcgta acaatgattt aattggtttg gtttcaattg
    13801 gttgtgcagc aagattaaga ccagtcttaa tgactacagc aacaacaatt ttaggtatgt
    13861 tgccaacagc actatctctt ggtgaaggtg gagaaactat gcaaccacta gccatagtta
    13921 taatgggtgg tctaactgta tctacactta taactttggt gcttatacct actatatact
    13981 taatatttga taaatgggaa aataagtttt cagacaaaat gcctaaaata tttggtaaga
    14041 taacagataa aataggaagt ataaaacgta aaataataaa gaaaccaaaa ttaaaagcta
    14101 aagtttaaat agaatggagg gtgattgtat tgaataaaaa aatgagcaaa atagttgcaa
    14161 ttggctgtgg tgttggtata ataactgcca atttaatccc agtttacgca gataataata
    14221 cttcttctat aaacaaagtt gataattcta ttttaagtga ctcaaataaa agaacatctg
    14281 aaaaggaaat tttgactgct agagaagctg ttagtgctgc acttaaaaat agtgaaaaat
    14341 taaaaatgaa atcagaagaa ataaaaatgt taaaagaaaa gttagaagta caagaggagt
    14401 ttgatagttt tactggaagt gacaactcgt ttccatacga ccaaattgga cttcttaaaa
    14461 atcaaagtga acaggctaaa ggctttatgg aggaccaaat agcaaatgat attactaata
    14521 aatttaacga tttagtttca agagaaaatg agctagataa gataaaaaac aatcttgaaa
    14581 taaaaactaa agaaattaaa gatatgaaat taaaaaagga tttagggtta gtaacatctt
    14641 tagaaactga atcagctgaa cttgaacttc aaacactaca aaatactcaa aaagctaaac
    14701 ttcaagaact aaaaaacaat caagactact ttaagttgtt gactaatata gatttaaata
    14761 attatcaatt ggataaagaa tatagatttg aatcttttag agtaggtggc tcagttgatt
    14821 catatatgga aggtaaggtt aatgaatatt taaaatacga ccaattgatt ttagaacgta
    14881 cagaagaaag ctttaatgat aaagatgaga acaaagcaga tttaccagat agacctaacc
    14941 ttacaagacc agttgctcct acaaaaccag agcaaggtaa cctaactgat gatgaataca
    15001 aggttcttat ggataagtat gaaaaagatt atgatagata taaaaaagaa gttgaagcat
    15061 acaatcttga aagacagact tatgctgctg gccttactac ttatgcaaat tatcttcaac
    15121 aaaaatttaa tactgaaaat ggtctagtaa cattagaaga cagtaaaaaa gctcttaaaa
    15181 aagggttaat tgatagctat gctcaattac ttgctttaga agatacaatt cagattacta
    15241 aaaaacaact tgatttatca gaaaaacaat taaaaaatac taaattaaga tatgatttag
    15301 gattaattac tctaactgat tataaaaaac aagtggtaag taatgaagat gctaaaaata
    15361 gctatgacac tcttattgtg aattataact cactaaaaaa tggtatagaa aaaccttgga
    15421 tacttaatac tggaaaataa ttctggtaaa tagaaagatt actttaacat acgtttgatt
    15481 aaaaaatagt tatgcatact cgtggcttta gtcatggaag attcagaagg caaataatag
    15541 aaatagatta gtaaaaatat atttacttat aaaactgtat acttttatag aggctatctc
    15601 aaaatagaga taagcctctt ttttattgtt taaattataa aaatatattt gattccatac
    15661 aaaaagagtg ccttctgaac taaaaagttc attttgagac accctcaaaa gcttaactct
    15721 aaatttaatt attttttatc aaatgtttca tctattttac atctcaccat gttaacaaat
    15781 tcagaaatat tatatcctct aagtttgcat ccacatttag gacaataatc cagtttccta
    15841 gataccaaag ccctacaact aggttctgga catcttattt ttagactctc atctgctact
    15901 ttaaatccac atcgtgggca tatactacct ggacgtgatg atacttcctt tccacattta
    15961 ggacactttg tcaactttat cccctccagt aaactcaatt taattgatag ttgaaatttc
    16021 tctctacaat aatatatgca atataattaa atacgtttca cttacaacta tataagttaa
    16081 atttttataa taaaaatccc ctatattcaa tttacaaata cttaatttga tacattgaac
    16141 aataggggat tttattttta tttagttaca tattttgcag tctattatta tctaggattt
    16201 tttatagatg cttgagctgc tgcaagtcta gcaattggca ctctaaatgg tgaacaagat
    16261 acatagttaa gtcctacact ataacagaac tctactgaag atggttctcc tccatgctct
    16321 ccacatattc ctagttttaa ttctggttta acttctctac ctaatttagc tcccatctta
    16381 actaacttac caacaccatt ttggtctagt acttggaatg ggtctttttc taatatttct
    16441 tcatctacat attgacctaa gaatttacca gcatcatctc ttgaatatcc aaatgccatt
    16501 tgagttaagt cattagtacc aaaactgaag aagtctgctt cttgtgctat ttcatcagca
    16561 gttaaacaag ctctaggtat ttctatcata gtacctacag tataaggaac ttctatacct
    16621 tcttctttta ttatagcgtc aacagtttct actatcattt ttcttattat ttttaactca
    16681 tttacttctc caactaatgg aaccattatc tctggagaaa ctttaacacc tgctttgatt
    16741 gcttctatag caccttgtat tatagcttta gattgcatta cacatatttc aggataagtt
    16801 attgcaagtc tacaacctct gtgtccaagc attgggttaa actcatctaa atcaactatt
    16861 ctcttcttaa tatcacttac ttttattccc atagattttg aaagttcttc tatagtctca
    16921 tcatcatgag gcaaaaattc atgtagtggt gggtctagta atcttatatt aactggtttt
    16981 ccatccataa ctttaaataa ctctacaaaa tcctctcttt gcattggtaa tattttttca
    17041 agagcttttt ctctttgttc tactgtatta gataatatca tttctctaac tgctggcaat
    17101 ctagcttcat caaagaacat atgctcagtt ctacatagac ctataccttc tgcaccaaac
    17161 tctatagctg cacgagcatc tctaggatta tctgcattag ttctaaccat catacatttg
    17221 tgtttgtcta cccagttcat taatttttca aaatttccag ttaaagatac ttctgttttc
    17281 ttaacatctc ctaggtacac atttcctgta gaaccatcta tagaaatgta ttctccttct
    17341 tttataacta catcatcaag tgctcttatt tctttattaa actcatcaac ttttatttct
    17401 ccacatccag caacacaaca tttacccata cctctagcaa caactgctgc atgtgacgtc
    17461 attccacctc tagctgtaag gattccttct gctttaacca ttccttctat atcttctgga
    17521 gatgtctcta gtctaactaa aactactttt tctccttttt catttgcttt tactacatca
    17581 tcagcattga aataaacttt accacttgct gcacctggtg atgctggtaa accttttgct
    17641 ataaccttag cttcttttaa tgccttatct tcaaattttg gatgtaataa ttggtctaat
    17701 tgattaggct caattctcat gatagcttct ttttcatcaa ttattccagc ttcaactaaa
    17761 tcaaccacta catttattgc tgctgctgct gttctcttac catttctagt ttgtagtata
    17821 tataatcttt cattttctat agtaaactct atatcttgca tatctttata atgaccttca
    17881 agtatatgag taatttttac aaattcatcg aatacagctg gcattacatc tgcaagagtt
    17941 gatatatttt gaggagttct tatacctgca acaacgtctt caccttgtgc attcattaaa
    18001 aattcaccaa ataatttatt ttctcctgta gctggatttc tagtaaatgc aacacctgta
    18061 ccactagttt ctcccatatt accaaatacc attgattgaa tatttactgc tgttcctaag
    18121 ttatgagcta tatcattcaa tttacggtat acgattgcac gaggattatt ccatgatcta
    18181 aatacagcct ctattgcaag cattaattgc tcttttgggt cttgtgggaa atcatttttt
    18241 atttcttttt tataaattgc tttaaattct tttactattt cttttaaatc atcagttgta
    18301 agttctgtat caactgtgta tccctttgct tcttttactc tgtctaatac attttcaaat
    18361 ttatattttg gaacttccat agcaacatca gaaaacattt gtatgaatct tctgtaacta
    18421 tcatatgcaa atctttcatt ttgtgtagcc tctgctaagc caacaacact attgtcatta
    18481 agacctaggt taagaattgt atccatcatt cctggcatag agaatactgc accagaacga
    18541 actgatacta ataatggatt cttattgcat cctaatgttt tatttaagtc tttttctaaa
    18601 gtagctaaat gctcttctat ttcttttatt atctctgctc ttatactttc attgttaaca
    18661 taataatcat tacatgcttc tgttgttata gtgaatccag gagggacagg taagcctatc
    18721 ttagtcattt ctgctaaatt ggcaccttta cctcctagta aagatttcat gtctttgctt
    18781 ccttcaccaa aactataaac atacttagtt tccattgtta aatcccccat taatcttttt
    18841 attttaattt atatcatttt tattaataga cattattaaa tttctatttc tttgtatatc
    18901 tttagaccat tttctttcat tatgtctaaa attatacctg ctgtttcttc tattgcctta
    18961 tttgaaacgt ttattactgg gcatccaatt cttttcatta tctcttctga ataatctaac
    19021 tcctgaagta ttctttctaa attagcgtag tttgctttac tggacaatcc taaagctttt
    19081 aatctttggg ttcttatctc attcagcttt tcaggtgaat tagtaagacc tattattttt
    19141 ttagtatcta tctcaaacac ttctttagga attggtattt ctggaactaa tggaacatta
    19201 gctactttta tatttttatt agctaaatac atactcaaag gagttttaga tgttcttgat
    19261 attcccacta aaattatgtc tgcttgaaga acacctctag ggtcttttcc atcatcatat
    19321 ttcactgcaa attctatagc ttcaactctc ttaaagtaag attcatctaa ttttctaatt
    19381 ataccagctt ctcttttagg ttttcttcct gttctcttgc ttatttctct tagtatatct
    19441 gtcattaaat ctatacaact aagaccttct ctttcgcaat atctttcaac aatactgcat
    19501 aattcttcat caactaatgt atatacaatt aaagcgtttt cagcttttgc attctccaaa
    19561 gtttcaagta gtactccttt attaatcatg tatggaaatc tttttatttc ataagtttca
    19621 ttcacataaa actgagacat acatgccttt gcaacttgtt gtgcagtctc ccctactgaa
    19681 tctgatactg catatataat taggtttttc accagcatac ctccttaatt gtcaactatt
    19741 tctaagaata gcttagttat gttggttttt gatattctcc caacaactct catatgattt
    19801 ttgtcttctc ccttatattc aacaatcgga atcgaatcta cttcatgttc tattattttt
    19861 cttgaagcta ataaaacact tgctccttta gttaaagtga ctacatttgg agttcttgtc
    19921 attatcatac ctattggcat tttatttata tcagaacctc ctatagttgc ttttagcaag
    19981 tcttttctgg aaacaactcc acataactct tcattctcat ctataataac tatactccct
    20041 acatcagata aaaacatagt gactattaca tcatatatac tttcatcctt cttagctagt
    20101 acaggcatgc tcattatatc ctctactgtt ttttctttta cacttttacc aattaaattt
    20161 atttcactaa ctcctgaata aaaatatcca acttttggtc ttgcgtctaa tattcctgtc
    20221 attgttaata tagctaaatc agatctaagt gtagcacgag tcacattcaa gctggaggca
    20281 atactttcac tcgttattgg ttcattttcc ttaactatat ctataatttt tagttgtctt
    20341 tcattaagct gaataataat caccttcttt cattgtgcta tactttttat cttttcgcat
    20401 cttatatagt atattatata actatatata ccattctgta aacagatttt ttattgaata
    20461 cgatatattt tttactaagt tctctcgctt aaagatattt attatatatt aatgggggat
    20521 atttctttaa ttaatagaac tatattagta ttacagttaa tattataata cataattggt
    20581 cattttaatt cattttatat taaaattttt aataatatta tttcaatgtg aatataaaat
    20641 tgcagaacta attcaaaata atttttacga ctacaaataa cctataatat acttttaaag
    20701 aaagtatcct aacaagaata tataaaaatt taaaagggtt gatttaaaaa taaccatcaa
    20761 ccctttttac taatatctat ttatattacc atcaaatctt acccaaatat tttttcttgc
    20821 aattgtctta aatgatataa agtagtatct ttttctatct ctaccatatc gtaagttata
    20881 acttcacctt ttttaatatc ttttacaact ttagtctttt tacttatcaa accaataggt
    20941 acagcattca tagcctttgc tacatcgtat ttttctatag taccatagca tgtatatgct
    21001 ccatagccat ccataaactc accttttttc aaatctttct tagctatagt cataacttct
    21061 gctactagtc cagctttagg tactattgta gccttatggt ctaagactgc catagctgct
    21121 gataaaggag tctctaaact acataaatga tatggtctat ataatatgta attaggtcca
    21181 tcacccatac ttaaatattt tagttcatca ttaactgctt ttaatttatg ggctactact
    21241 acgaaaactc caggtgctat accatttaca aaatcaacta caccataatt atctaatact
    21301 ccaccttcgc tttttaatgc atatttctta ggtacttcat taactgtagc ttcaatacca
    21361 tgaccacctc ttacatcagg cacaaatcca gttgcattac acattaaagc catttctacc
    21421 attgttttag ttccttcttt aaaagaagct atcatatgag gtgaagctcc ttttctttct
    21481 gcttcttctc taactgtatc aggattacaa tctaaatcta atttattatt tttaccttta
    21541 cctatcactc taacttcaaa ccccattgca tctgcaaaat caaataattc cattactgca
    21601 cctggctcat ctcctgcaga acctgtaaat attactccag catcatctgc taatttttta
    21661 agtattggtc caacaacaca atcagtttca gcatttaaca taactatatg ttttttattt
    21721 aaaatagagt ttaatgctat ctctgcacca attgcaatac ctccagttgc atctataact
    21781 acatctattg acttagtttt acaagccaca agaaaatcat tacatgtcaa tacttttcct
    21841 tcttgtaatt ttatttctgc atccttttca tttgttacat tatctataat ttcttcatct
    21901 attcctgcat cagtaaaagc tttatgagcc aactccaaat ttatatctac aacaatagaa
    21961 gcttcaatac ctttcatatt tgccatttgg cttaccattc catttcccat atgcccagct
    22021 cctataagag ctacttttat ttttttacct tcctcttcca attttgcaag ttttctgttt
    22081 aaatctaaca taccaattcc tcctgttata ataattttat tttcaaatta agaaatagtt
    22141 attaaatctc caatagttat atctggattt cctatttcca catgtatttc tcctggtaaa
    22201 tttacttcac attttccatc aaatttgatt gtacaatgcc caatttctct aagagtcttc
    22261 tgagcaatat caccaactga aactatacaa taatctctat tccctatagt aagtgtatct
    22321 cctatttcaa catcactttt taacgttgct attgaatgaa ttacagatat atcctttaag
    22381 tcgtcatcat taatggtttc atcatatata ataagacaat tgctttcatc cattaactct
    22441 ttaactaaac tgcctatccc acttatctta acttcatatt tcatgacatt cctcctgata
    22501 atttatattt atattgagtt aattaaaaga aacatttatt ttttctttta taaattttat
    22561 agcttgtaca atactctctt catctttaaa tttttgctct gcactatata tatctaatcc
    22621 ttctatttct tcaatctctt taaatttagc cattactgta cgtcctttta aaatttctcc
    22681 aaactttata aacccatctg aatctgaaga tataatggct atacttcctt tagaaaaata
    22741 gcttttcttt ttgcctgtag acactatatc accctttttt ttagctctat ttaaagtttt
    22801 aaaatacaat ttattctgct tcattataaa tatagtattt aaaatataag caacaaataa
    22861 aatcactaat aatttcatat ttatacacct tccaaaaatc acattatttt taaatctgta
    22921 taacaactat aaatgctgta caatagttat ataaggtttt taagtaacac ctttttaaat
    22981 tctaaaaaag ttctacattt acttatctta ttcgttaatt ttacatcttg gcctatcttt
    23041 aaaacttctt cataaatttt tactaataag tctgagtcta tcttattctc acatggaaac
    23101 attgtcataa gaattatttt tatacttcta tttgcatatt ctattggttc ttctaaaatt
    23161 ccaactgcca ttaaaaactt atctgatttt tcatttatag aatgaggaaa taataaacct
    23221 ttatcaaata ctgtgggaga tttttcttct ctattgacta tattttttct aaacttatca
    23281 tcaatgcatc ctaaatccat aagatggtcc atcatttttt ctaatgaagc aagtatagtc
    23341 ttttcattta atatcataaa tttatcttca tctaacaaat tatttattag taaatttgta
    23401 gaattagtag tgtcaatatt cccttctctt aggcacaata ctgtttttaa ttgttctctt
    23461 agtttgtttt catcaaatat tgtatttatt tttaatacta ttgtatttat atttgatgtg
    23521 tctaaatcaa tagttgtaaa aactatatca tattcatcta agttaatatt tctcaaatta
    23581 aaacttgaaa gagtatcaat tttaggatta tttcctatca gtttttctac tcttattttc
    23641 aatagttttg ctgttcctag acctgtacta catataattg ctattttttt tattgaatag
    23701 aatctactgc tatttctttc taaatatcca ctgaaatgga ttgctatata tccaatctca
    23761 tcttctgaaa cttctaagtt atactttttc tctattactt ttcctgctat ttgtgctaat
    23821 ttaaatggaa gtatataatt ttgcttaatc tcttctaaca aaacattttt taactttata
    23881 ttaaataata gcctgtttaa agtaaaacta agatgatatc ctagacctac tcttaattca
    23941 tcctcattta taaaaatacc catatcgaca tatatttgat taaatatatt ttccatcaac
    24001 tcatcaatgt ttttactaat tctactacta tttaaagcac attctgatgc tggtgcattt
    24061 ccactaacta acggtatagt taaataaata atttcttcat catttaaagt acattcaaac
    24121 attcctgata ttatatgttt tatttcccta gcaataaagt attcatcata tgatttaagt
    24181 aaatcaaact ttacatcata atcttttact ttcttttctt cttttactct atagactaaa
    24241 actactatgt agttaataac ttctcttaac atttcatctg taacataaaa attatttttt
    24301 ttaaataaag tcaatagttt ttcttctaaa tttatgattt cttctttttt gattccttta
    24361 aaatactttg aattactgaa atctttcctt gagtttttat acaactgatt taatataaac
    24421 agtctaatat caagttcatt tccatttaat ttcatacctg tattctgttt tttaatcaag
    24481 tttaaattat atgaagccaa aactttttct acatactgga aatcatttac taaagtagtt
    24541 cttcctacac acatctcatc agctaaacta tcaagtgtaa taaatccatc tagttctatc
    24601 aagtaattta gtataaactc tactctatct tcttttgaat ttaatgaaaa tacatttttt
    24661 ctattcatat caataatctt attgagtatc tgctcatctt ttatttctag tttatagcct
    24721 tgacccttta tatatttgat tactgctata tcattaccta aatctttatt caattgtttt
    24781 atatacgtcc ttacacttct agttgtgaca ttcaattcat ttgctattga gttaatactc
    24841 acaaactctt ttttactaca cataatcaat atcttaaata tcttgcggtc aatagaagga
    24901 attttctcag ttacactcat tttgtatctc ctccttgatt tcattctagt acaaatttga
    24961 aaccaaatta atacataaaa tttcacttta ataaaggaaa atttagtatt aatctaactc
    25021 gataattaca gctaattaat agtataaaag ctagtctttc attaacaaaa aatcttgact
    25081 agcttaaatt tatatttaca attttattaa ttaattccaa gccttatatt gacattaaat
    25141 attatctcaa tgcaaatact gcaactccat atgcaataat aacagctatt ggaccagtta
    25201 ttaaacgaga gtaaaataaa gctggaacac catactctac agtttctggt tctgcttctc
    25261 ccaaacttaa tcctactggt acaaaatcac atccagcttg cccatttatt gcaaataaag
    25321 ctggaagtgc taaatatgct ggtattgcac caagaccaat ttgtgtacct acaagagtcc
    25381 ctactacttg agctataact gcaccaggtc ctagtattgg tgataagaat ggaagtgtgc
    25441 aaattactga tataatcaat aatccaccaa tattacctgc aagtggagat ataactcttg
    25501 caatccagtc tcccagtcca gatgctaaaa taattcccat aagcatactt acaaatgcca
    25561 taaatggtat tacgttattc attaccattt ggatagtatc tcgacctgca tcataaaatt
    25621 ttgatactac ttgccctgcc ccttgaccaa tagaaagtat tatgttcttt ttagcatatt
    25681 caccttgtgc ttttgactta acctcatcat aattatcagg ctttctaata gaactcttat
    25741 tttcgctttt attttcttga gatttttgtg gcatatcttc cccatctaca acacttatac
    25801 agttaggatt aacatctgat acataatatt cttctgttat aaattttgct aaaggacctg
    25861 tttttccaac tggatttaca tttattgtag gtattttttt ctttggataa actccacatc
    25921 tagcagttcc accacaatca ataataacta ctgctacttc ttcatcagaa actccagttt
    25981 taaatccatc tacaatttca caacccagta gttcttttat ttttaatgtt acttcatgga
    26041 taccaccagc agtcattgac agtatcttat ttctattccc ttcttttttt atatacaaag
    26101 gacctcccca gcctgaagtt cccttttcta tttttaatat tttattctcc tccattttat
    26161 ctacctccta agccttattt gttgctctat tttttcttgt tagaaaagca tacatttttt
    26221 ctgttactat acctcttata aaaattacaa ttagaccaac tataaaatac cacaatgcca
    26281 atgtagtagt ttctttacct aaagtagtca aacctgcaga tattcccaac catacaaaaa
    26341 gttctcctgc atttgcatgt ggaaaaagac ctgtaattgg atgtacaaat gaaactgctg
    26401 catcataaaa tcctgctttg tgctcttctt ttaaaaatct accaaatgta tagcacatag
    26461 gatttcccaa gaaaaatact gatactaatg gaagtagaat atatcttgta aaagagttct
    26521 ttcctagctt agaagcccaa ttattaactc tttcctcacc aattatctta atcaaagagt
    26581 taatagctac cattaataca attagcgttg gaagagtacc tgaaacaaga cctataaatt
    26641 gttctccccc tttctgaaac aaaccgataa accactctgc acctttactt agacctgtaa
    26701 taacgttttc cataaaatta taacctccct ataatatctt taaattttta attttactgc
    26761 ttaccataaa ttttacaaat tacatctttt ttctgtacta tatgctaata ttgtaaatgt
    26821 ttttttatta atatcatttt aatataatat ttttgataaa taaatacatc t
//
LOCUS       DAEORZ010000043        26290 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEORZ010000043 DAEORZ010000000
VERSION     DAEORZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.043"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            79..1290
                     /locus_tag="KRH39_001653"
     CDS             79..1290
                     /locus_tag="KRH39_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBG8511566.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            1589..3241
                     /locus_tag="KRH39_001654"
     CDS             1589..3241
                     /locus_tag="KRH39_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG8511567.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKAPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(3444..5231)
                     /locus_tag="KRH39_001655"
     CDS             complement(3444..5231)
                     /locus_tag="KRH39_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBG8511568.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSEQILH"
     gene            5527..6561
                     /gene="ltaE"
                     /locus_tag="KRH39_001656"
     CDS             5527..6561
                     /gene="ltaE"
                     /locus_tag="KRH39_001656"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBG8511569.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALNLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            6848..7678
                     /locus_tag="KRH39_001657"
     CDS             6848..7678
                     /locus_tag="KRH39_001657"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBG8511570.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            7682..8563
                     /locus_tag="KRH39_001658"
     CDS             7682..8563
                     /locus_tag="KRH39_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBG8511571.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            8593..9210
                     /gene="gmk"
                     /locus_tag="KRH39_001659"
     CDS             8593..9210
                     /gene="gmk"
                     /locus_tag="KRH39_001659"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBG8511572.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            9213..9479
                     /locus_tag="KRH39_001660"
     CDS             9213..9479
                     /locus_tag="KRH39_001660"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBG8511573.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            9484..10683
                     /gene="coaBC"
                     /locus_tag="KRH39_001661"
     CDS             9484..10683
                     /gene="coaBC"
                     /locus_tag="KRH39_001661"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBG8511574.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            10922..13447
                     /gene="priA"
                     /locus_tag="KRH39_001662"
     CDS             10922..13447
                     /gene="priA"
                     /locus_tag="KRH39_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBG8511575.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFIKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENIEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPDIEVVDMKDELDKGNRSIFSL
                     KLQKEIHYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            13465..13905
                     /gene="def"
                     /locus_tag="KRH39_001663"
     CDS             13465..13905
                     /gene="def"
                     /locus_tag="KRH39_001663"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG8511576.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            13920..14849
                     /locus_tag="KRH39_001664"
     CDS             13920..14849
                     /locus_tag="KRH39_001664"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBG8511577.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            14859..15611
                     /locus_tag="KRH39_001665"
     CDS             14859..15611
                     /locus_tag="KRH39_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBG8511578.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            15680..16381
                     /locus_tag="KRH39_001666"
     CDS             15680..16381
                     /locus_tag="KRH39_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBG8511579.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            16443..17768
                     /gene="rsmB"
                     /locus_tag="KRH39_001667"
     CDS             16443..17768
                     /gene="rsmB"
                     /locus_tag="KRH39_001667"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBG8511580.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            17776..18807
                     /gene="rlmN"
                     /locus_tag="KRH39_001668"
     CDS             17776..18807
                     /gene="rlmN"
                     /locus_tag="KRH39_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBG8511581.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGMVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            18827..19579
                     /locus_tag="KRH39_001669"
     CDS             18827..19579
                     /locus_tag="KRH39_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBG8511582.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            19596..21611
                     /gene="pknB"
                     /locus_tag="KRH39_001670"
     CDS             19596..21611
                     /gene="pknB"
                     /locus_tag="KRH39_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBG8511583.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            21719..22456
                     /locus_tag="KRH39_001671"
     CDS             21719..22456
                     /locus_tag="KRH39_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG8511584.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            22554..23438
                     /gene="rsgA"
                     /locus_tag="KRH39_001672"
     CDS             22554..23438
                     /gene="rsgA"
                     /locus_tag="KRH39_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG8511585.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            23443..24114
                     /locus_tag="KRH39_001673"
     CDS             23443..24114
                     /locus_tag="KRH39_001673"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG8511586.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            24123..24764
                     /locus_tag="KRH39_001674"
     CDS             24123..24764
                     /locus_tag="KRH39_001674"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBG8511587.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            24895..25524
                     /locus_tag="KRH39_001675"
     CDS             24895..25524
                     /locus_tag="KRH39_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG8511588.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            25579..26235
                     /locus_tag="KRH39_001676"
     CDS             25579..26235
                     /locus_tag="KRH39_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG8511589.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT        10452 a         2687 c         4912 g         8239 t
ORIGIN      
        1 atctagggaa tgataattaa ttaaaagata ttaattaaac agaggattta tttatatata
       61 ataattttgg aggtttttat gaaaaaaacg ataatgtctt tacaacatct attagccatg
      121 tttggagcga cagtattagt tcccattttg acaggtttta atccgtcagt tgcaattttc
      181 tgtgctggtg ttggtacatt gatatttcat ttttgtacag aaggaaaagt acctgccttt
      241 ttaggttcaa gttttgcgtt tataccagtt atactagcag ctaaagaggc gtatggtgga
      301 gatttagcat atgctcaagg tggtataata gtagcaggat taatttatat tataatgtcg
      361 attatagtaa aagtagttgg tgtaaataaa attaaattat attttcctgc acaagttaca
      421 ggagctatga tagtagttat aggattaaat ttattgccaa cagcttttag tatggcttca
      481 gccaattttg tgatagcatt tataacttta gcaatagcaa ttttaactaa taagtttggt
      541 agaggattta taaaacaatt aggaatatta atagcagttt tttctgggta tataatctgt
      601 ttgatattaa gattagtaga tataactact attacagaag caagtttatt tgcaatacct
      661 aactttacag ttcctaagtt cagtttagga gcaattgtga ttatatctcc agttgtatta
      721 gctgtattta tggaacatat aggagatatg actactaatg gtgctgtagt aggtaaaaac
      781 tttatagaaa atcccggatt aaatagaaca ttacttgggg atggatttgc gactatagtt
      841 gcagggtgtt taggaggacc agccaataca acttatggag aaaatacagc agtacttgct
      901 ataactaaaa actatgaccc ttcaatactt agacgtacag caatatttgc tatattgctt
      961 gcatgtgtag gcaagtttgg tggatttctt caaagtatac caggttcagt tatgggtgga
     1021 attagtataa tgctattttc gatgataact tatgtaggat taaaaactat aagggacagt
     1081 tcttgtgtag aaagtaaaat aaatatatta atcatagcag ttatattatt aataggtctt
     1141 ggaacaacat atttatctaa taaaggtatt tctgttggaa tccctataac aagtacagta
     1201 aagataactg gtcttagttt agctgcaatt gttggaattg tattaaacag aattttaaat
     1261 aatcaggact ttaaagttga agaagaataa atttaaagaa taagttgtat attgaataac
     1321 taaaattagt taattttcta tagacatttg taattaatgt tgatataatg attaaaaata
     1381 aatgaaagaa agggcaaggt aactaggaca atgagaatat aatctatttt ttataattta
     1441 aagtattgta atttttgaaa agcctatttt ggtggcttta tttgcaatgc ttaaattaag
     1501 gagagtagat tatagtctat acgtttattt gttatgccta tttatagaat ctctccttat
     1561 atttaagtgt gttaaggagg gattttttat gttgttagta aaagtagaga atttaaagaa
     1621 atattatgca gataaattaa ttttggatat agataaactt gaaatattag aaaatgacaa
     1681 aataggtcta gttggttcaa atggtcaagg aaaaacaaca ttattaaaag caatattggg
     1741 agaaatagaa attgatgaag gatatactta ccttacagaa agctattctt atataagtca
     1801 gagtgaaaat aatattgaaa catgtagtca tagtaaggag aagagtcttt taaatgctcc
     1861 agataaattt gaagaacact tatcaggagg ggaaaaggtt aagttaaaaa tagcagatgc
     1921 gctgagtaat aagaaaaata tcataatagc tgatgagcca acttcaaact tagataaaaa
     1981 aagcattggg gttttggaag atatgtttaa aaggcatgag ggagcattat tattaatatc
     2041 tcatgataga cgttttctgg atgagttatg tacaactata ttagaactag aagatggaaa
     2101 actaaaagct tacaaaggca attacactga ttatttaatg caaaaagatg aagaagtaaa
     2161 aagagctgat tttgagtatc aagaatatgt taaagagaaa aaaaggcttg aaaaagctct
     2221 tttatataaa aaagctttaa gtgatggtat aagaaaagca ccaaaaagaa tgggtaattc
     2281 agaagctagg ttgcacaaaa tgggtggtca gactaataag aaaaagttgg actcaaatgt
     2341 gaaggctata aaaagcagaa ttgataaact tgaggtaaag aataaaccta aagtttctaa
     2401 agaaatgaac attaagatgc aagatggtat ggagataatt agcaaaaatc tagtagaggt
     2461 aaaagatatg actctaaagt tagaaaataa gctcctgtta gataatgttt cctttaagat
     2521 aaaaagaggt aaaaagatag cattgttagg tgataatggg tgtggaaaaa gtactctgat
     2581 aaaagaaata ttggctgata aaaatgacaa tataaaaata aataacaagg taaaagtagg
     2641 ttattttgac caaaatcaaa gtttattaga tgaggaaaag agtgttctat acaatactaa
     2701 agttaatagt tcatttgatg aatcttttat aaggataaac ttaagcctat ttgggtttaa
     2761 aggtgatgat gtctacaaaa aagtaaaagt acttagtggt ggagaaaagg taaaaatagc
     2821 actatgtaaa ataatattgg aagataataa ttttttagta tttgatgaac caacaaatta
     2881 cttagatata aaatctatgg aagcactgga aaaggcactg ataaatactg ataaaactat
     2941 gcttatagtg tctcatgata gagtatttgt atcacatatt tgtaactata ttatagagat
     3001 aaaagatgct aaaataaggg agtttgattg taattatgac gaatatacaa ttagcagaaa
     3061 taaaaaaact cctagtagag aaaaccaaat caaaaaagaa aatcttctag tattagaaaa
     3121 tagacttaca actgttatat ctatgttatc tatagaaaaa gataatctaa aaaaagaatt
     3181 gtatgaatca gaatataatg aattactaaa acaaatcaat aaattaaaaa atagctttta
     3241 gtgttaactt tttaattaca tctatataag aagactgaaa atatttgttg ggaatataag
     3301 tatagttatt aaaacagaaa agtattttgt tagaaaatta aaagtaagct aattagaaag
     3361 tggccttatt attgtacttt ataagaaggc cactattttt atttagaatg ataatgtgta
     3421 gttgtctaac atttattttg tatttagtgc agaatttgtt cagatttaac cttaagcttg
     3481 gctactgttt cttcttcagg agtaacataa atagtactgt tagtttcata aataaccata
     3541 cctggtttag aaccactagg ttttttcaca ttttttctct tagtatagtc tacaggcacc
     3601 tgagaagaaa gttttgactt actaaagaat gctgctagca tagctccttc aaatacagta
     3661 ttatcaggga cttccttacc agcacacttt attattacat gagaaccagg aatattcttt
     3721 gtatgcatcc ataagtcatc attatcagca agtcttagtg taagatagtc attttgctta
     3781 ttgtttttac caaccaatat tttaaatcca tcagaagaaa caaattcatg aggttttgta
     3841 gatgggatag tatccttttt actgtttttc ttttgagtct tgatgtatcc aactttagca
     3901 agttcttctt ttatatcttg taattcagct aaattttcac agttttcaat acttaaaatt
     3961 atgttttcta agtaatctat ttcttcctta tttaaggaaa tttgatgaga tatctcgact
     4021 ttagcatgtt tcattttgtt atacttttta aagtattttt gagcattttc agatggtgtc
     4081 aagtttttat ttaattctat tgttacatca ttacaatttt catcatagaa gttagcaact
     4141 tcaatacttt ccataccttt ttctaccata taaatatatg aagtaatcaa ttctcctttt
     4201 atcttataaa tgtctgcatt ttcggattct gataattctt cttcttgttt tttaagttta
     4261 ttataaagtc tatcaagctt aacagatata ctcttcttta aatcagatga acgttgatta
     4321 attctatctt taatatcctt agttctatag aaatcctcca gaatacgaga catactatcc
     4381 ttatttatat atgataaatc gctaaataat gtaaggttta tacaactgaa atcaactacc
     4441 ttatccacct ttttatctat aataatgcaa ggagagtatt tgttagaatt tatatcattg
     4501 aataaattac aaaatacttt gtgtaaagaa tcaaattgtt catcagatat atcctttatg
     4561 atagcatttt gatttacacc tgctctgtag caaatttctt tagctataat agggcttata
     4621 cctagaaatt tagaatatat agacttaaag ataggtcctt ccaattcctc taggttttta
     4681 ataaacaaat ccttagatat atcatcaaga gggttgagtt tattttgttc tggtggtaat
     4741 gaataatcat gaccaggtaa aacttgacga actctactta tgctaaaagg gactcttttt
     4801 atagaatcta taattttatt ttcttcacca tgagttatta ttatgttgct atgtcttccc
     4861 ataatttcaa tcataatatt tttaactgtc ttttctttta attcatctaa agattcaaca
     4921 cttattttta ttattctttc aaaacctatt tgagaaattt ctactatatt tccaccttgt
     4981 atgtattttc taagtaacat acaaaacata ggcgctttta gagggttttc tttttgataa
     5041 ttacttgtaa gataaactct aggatttgag gcacttgcac ttaaaaccaa tctaaaattt
     5101 tctttattat ttcttatatt aaaaattacc tcatcatctt caggttgatg tatcttatct
     5161 atttttccac ctgtaagttt tgatgataat tcatcgacta ttgaatgtat aactaatcca
     5221 tctaatgcca tttatagcac ccctttcttt gaccaaaatg agtcagtaaa ttaattatac
     5281 acataatata cgctttgtga ctagggggaa tttaaacaat agagattaat atttaaattg
     5341 tatgattatt tatatttatt agagaggaaa ctttattttt aaaattaata atttaaagta
     5401 gattaaatat aaagtaaata tagtataatt attgtgtaga gtataagcta tgaaagtata
     5461 tgaactttac agaaaaaatc aattttaagg ttctatagta tataaatatg aaagaaggtt
     5521 gaaaaaatga gatttatcga tttaagaagt gatactgtta ctatgcctac tgacgaaatg
     5581 agagaagcta tggcaaaggc tcctgttgga gattcaatct ttagagatga ccctactgta
     5641 aataaacttg aagaattagc agcagctaaa gttggaaaag aagatgctat tttccttcca
     5701 agtggaactt ttgggaatca attagctcta tttactcatt gcctaagagg gcaagaaata
     5761 attattggta aaggttatca cattgtgact catgaggttg gggcacctgc tgtaatagct
     5821 ggagttcagc ttagaactat agatgaagac gaaagtggag ctctaaatcc tgaacttgtt
     5881 gaaaaggcaa ttagaagaga tgacatacat gaaccacaga cagggcttat atgtgtagaa
     5941 aatgcatatt caggtggaac agttgtgagt cttgataatt taagagaaat taaaaaaata
     6001 gctgagaaac ataacttacc agtacattta gatggagcaa gattatttaa cgcagcatta
     6061 aatttgggtg tagaagctaa agaaatagca aagtgttgtg atagtgttat gttctgtgtg
     6121 tcaaaaggtt tagctgcacc aatgggttct atacttgctg gaagtaaaga atttatagca
     6181 aaagctagaa gaaagcaaaa agttatgggt gggggaatga gacaagtagg tatagttgca
     6241 gcagctggta taattgccat tgaaaaaatg actttaagat tgaatgaaga ccatgaaaat
     6301 gcaaaatatt tagcttgtga attaaataaa attgatggca ttgaagtaaa taatgttaat
     6361 ccagatataa gtatggtatt ttttaagatg agtgaagata ttataaaaga ggatgtttta
     6421 ataaaagaat tttttgaaag aaatataaaa attaataaaa tggaaaatgg tgaatataga
     6481 tttgtaactc atgtagatat tactaaggaa gatttagact atgttttaaa aacacttaaa
     6541 gaattaatag gtgtatgtta atagatatct actagaagga ttatagctag ttaccagatg
     6601 tttaattaaa aaattcatag acagtttaaa taaaacatac ataaaattca tatagaaata
     6661 ttaatatata aacatagaaa gaactagctt aaaaacataa tataattacc ctaattgtaa
     6721 tggcaactct ccctcacatc ctcaaattat agttgtcatt acaaaacttt tcccataaat
     6781 tataagttta tgggaatttt tttatgttat aatagtttaa gcgttaattt acgaaggagc
     6841 gatacttatg aaattttgga aactacatgg aataggaaat gattttatag caatagatgg
     6901 aagattcgac cagataaatc catctgatta tactgcttta gcaaaaaaag tatgccatag
     6961 aagattttct gtaggagcgg atggattgtt agttgtgaaa gattcaaaag ttgcagatgt
     7021 agaaatggta tattataatt ctgatggttc aagagcagct atgtgtggaa atggccttag
     7081 atgtttttgt aaatttgttt atgataattg tatagttaaa aaagaaagtt ttacagtaga
     7141 tacattagat ggtataaaag atataaaagt aaatcttacc aatagagaaa taaactctat
     7201 aagagtcaat atgggaaaag gaagttttat agctaaggac gttcctgtac ttacaaataa
     7261 agaacgtttt gtgcaagaaa gtataaaggt tttagatagg gagcttaaag ttacttctat
     7321 gttgatgggg gttcctcata cagtagttat agtagatgaa ttagatgtag atgatgtttg
     7381 taaatatgga aaagagattg aaaataataa aatatttcct gaaaaaacga atgtaaattt
     7441 tgtaaaggta gaagataagt ataatataca tgtttatact tgggaaagag gttgtggata
     7501 tactttaggt tgtggtactg gtatgactgc atcagcaata gtgtgcaatt tacttaatat
     7561 ggtagaaagt agtgtaaatg taacgtctca aggtggaaca gttaaaatag atgtaggaga
     7621 tttctcttat atgacaggtc cagcagtaaa aatatgtgaa ggtattttgg aggtttaatt
     7681 aatggctata agtatgactg gttttggaag aggagaatac aaggatgata attattactt
     7741 tttagtagag tgtaaaacga taaatcataa gtattcagat ataaacatac gtcttccaag
     7801 aaaaatttca tttttagagg ataaagttag aaatttagta aaaaattatg taaaaagagg
     7861 aagagtagat ttatacataa agtttgactt attaggcaaa gaagatgtaa acttaaattt
     7921 tgatgaggga ttagcatctc agtatataga catactaaaa gaaataaaaa ataaatttga
     7981 tattatagat gatattagcg ttatgaatgt tgcaaagttt cctgatattg tgaaaataga
     8041 agaaaaggaa gaagatgaag atttattgtg gtcaatgttg aatcaagcag ttgaagatgc
     8101 attaatcaaa ttaagagaaa tgagaagtga agaaggtaaa aaattagctg aagatattgc
     8161 catgagatgt gatttattaa aaaatcatat agaagagatt gaaaaatatt caagtagtgt
     8221 tgtagaagat tatagagaaa aattgaattt gagaataagt gaattactgg atgacccaag
     8281 tataatagat gagaatagat tagcacaaga agttgcaata tatgcagata aaagcagtat
     8341 aacagaagaa atagttagat ttaaaagtca tatagggcaa cttaaaaata ctatatttaa
     8401 agacgactct ataggtagaa aaatagattt cttaatacaa gagatgaata gagaaactaa
     8461 cactataggt tcgaagtcat ctgatataaa tataactaat ttagtagtag aagtaaaaag
     8521 cgaattggaa aaaataagag aacaaataca aaatatagag taagaataca atattagaag
     8581 gggaatgctt aaatgtttgt aaaaaaagga ttattgctag ttgtatcagg accatcaggt
     8641 gcaggaaaag gtactatttg caaagaacta cttaaagaaa atgatacaat aaaactttct
     8701 gtatcagcta ctacaagaaa acccagaact ggagaagttg atggagtaaa ttatttcttc
     8761 ataagtaagg aaaagtttga agagatgata gaaaaaggtg agtttttgga gtatgctcaa
     8821 atttatgata atttttatgg aactccaaaa gcggcaataa tggaatgttt agaaaaagga
     8881 caagatgtat tactggagat tgaaatgcaa ggagcaaaac aaataaaaga agtttgccca
     8941 gagggagtat ttatatttgt actaccacca tcacttaaag aattaaaaaa tagaattgtt
     9001 ggaagaggta cagaaacaga agctgaaata gagaagagat ttagctgtgc atatgaagaa
     9061 attaaaatga ttaaggatta tgattatttt atatttaatg aagatgtaaa aacatctgct
     9121 aaagaaatag aaggtataat atcatctgaa aagaataaag ttagcagata taaaaatatt
     9181 ataatagaga aatttaagga ggaattataa ttatgttaaa accatctatc aatgaggtac
     9241 tagaaaaaat agacaacaga tactatttag tagggactgt ttcaaagaga gcaagaaaat
     9301 taattgatgg agaagaacca tatgtttcaa ataaaactaa ggaaaagcct gtatgtgtag
     9361 caactaaaga agttgcaagt ggaaaaataa catatagatt attgacagaa gaggaaatag
     9421 agatagaaga agctagacat catgctgagc aacaccaaca aatttcagaa gaggagtaag
     9481 gtcatgctaa aagataaaac tgttgtaatt ggcataagtg gcggtatagc tgcatataag
     9541 gcttgtgatg ttgtaagtaa gttaaaaaaa ctaaatgcaa atatccatgt tataatgaca
     9601 aaatcagcaa cagaatttgt taaaccactt acatttcaat cgctgagtca aaattatgta
     9661 gtagaagata tgtttgaaga accaaaaact tgggaagttg aacatatatc attagcaaaa
     9721 aaagcagatt tattcttaat agtgccagca agtgcaaatg ttataggcaa gattgcaaat
     9781 ggtatagctg acgatatgtt gacaactact gttatggcaa caaaagcaca ggttcttata
     9841 gcaccagcta tgaatactaa tatgtatgaa aatccaattg ttcaaaaaaa tatacaaact
     9901 ttaaagagct ttggatataa atttattgaa ccggaaagtg gaaggcttgc atgtggagat
     9961 attggaacag gtaaactagc taatccagat ataatagtag aggaagtttt gactaatctt
    10021 actaaagagc aagatttaaa aggtcaaagt atgatagtta cagctggtcc aactgtagag
    10081 agtatagacc cagtaagata tttaactaat aggtcttctg gtaaaatggg atattctata
    10141 gcaagagaag ctatagctag aggtgctgat gtaacattaa tatcaggtcc tacaaatctt
    10201 tctattccac aaaatcttaa aaaatttgtt caaatagagt cagcacaaga tttgtatgaa
    10261 gcagtagtat caaatttaag tgaaaataaa atagtaattc aaagtgcagc agttgcagat
    10321 tataaaccaa aaacttattc tgacaaaaaa attaaaaaga agaatgatga tttatcaata
    10381 gaactaagta gaaattatga tattgcttat gaaataggaa aaataaaaga agataagata
    10441 ctaattggat ttgcagctga aactaatgac cttatagaac atgccaaagg aaaaataaaa
    10501 aagaaaaatt tagattttat tgtggcaaat gatttaacta aagaaggtgc tggttttaga
    10561 gctgatacta atattgtaaa aataatagat gtagagggaa atattactga gtatcctaag
    10621 atgaaaaaag aagaagtagc caatgttatt ttagataaag ttaaagcgtt gcttgagaaa
    10681 taagaccttt tttaaatatg atatttgtgt attttatcac agctaaagtg gctgaaagct
    10741 gcaatttaat tttagcgagt aaaaactcgc aaatgtcagt taaattgtag ttttcagcat
    10801 ttattttgtc ataaagtata aaatataatt aatagttaat tattaaaata aagttaatat
    10861 taataacttg aaattgctta taaattctga gattttaata tcttgtaaag tatattaaaa
    10921 tatgaacaat atattaaatt taagtagagg ttttataatg aaaaaatatg caaaggtaat
    10981 agttagaagt aatactatat atacagataa tttatttaca tatcaaattc ctgtattttt
    11041 atctgatgta ataaaaattg ggcacagaat attagtacca tttggaaaag gtaataaacc
    11101 aacagaagcc tttgtatttc aatttacaga tagcttagat gaaaaaataa aaataaaaga
    11161 aattattgat atattagatg aaaatccgat atttataaaa gaagatttag agttggttta
    11221 ttggatgaaa aatagatatt tgtgtactta tatagaatgt ataaatttga tatatccaaa
    11281 gggatacaaa ttaaataatt ataaagtagt cctattaggt gaaagtttaa gtggattaaa
    11341 tgatattgaa ttgaaggaaa aaatatcaac tttaagtgat aaaaatagag aaatagtaga
    11401 gaaagtgatt gatagtaaag gtaaaattaa ggttgataag ttaaaatata taccaaactt
    11461 aaatagttct ttgtacacaa tgaataaaaa tggaattata aaattatgtt gggaatataa
    11521 aaatcacaaa aatgaaaaga aggtatgtta tatatctttg agtttagaaa gtgataagat
    11581 tgacgattat atagagcaaa ataaaataaa tgtgggaagt aaacaaaaag agatattaag
    11641 ttttcttaaa aataatgaaa atatagagat aaatgattta ttggatttat taaatgcttc
    11701 aaaacaaagt ataaattctc taagtaagaa aaagctaata accttagaat ttaaggatta
    11761 ttatagagag ccaaaaagta tttacaaatc cgtactaaaa agtataaaat taaacaatga
    11821 gcaacaagaa gcagttgatg agataaagtc gaatatgttt gtagatgata aaaaaccata
    11881 cttaattcat ggagtaactg gaagtggaaa aactgaagtt tacatggaaa taatagaatt
    11941 tgctctaaat caaggcttag acagtatatt cttagtacct gagatagcat taactcctca
    12001 aacaatagat aggctaaaga gtaggtttgg agatttggtt ggagtgttcc acagtaaact
    12061 atctgaagga gaaaaacatg atgtttacaa agctgtcaaa gcaggtaaag taagagtgtt
    12121 aattggagct aggtcagctt tatttgcacc ttttaattcc ttaggcttaa taataattga
    12181 tgaatgtcat gagtcatcat ataagtctga gaagaatcca aaatttaatg ctatagaagt
    12241 tgctagattt atggcattaa aaaataatat aactcttatt ttaggttcag caacgccttc
    12301 aattgaagag tattataggg caaaatctgg agaatacaaa cttataaata taaaaagtag
    12361 agcaaatgat aagcctcttc cagacattga agttgtagac atgaaagatg agttagataa
    12421 aggaaataga agtatattta gtttgaaatt acaaaaagaa atacattatg cgattgaaga
    12481 aaataatcaa gtaatactat ttttaaacag aagaggttat gcaaattttg tatcttgtag
    12541 aaagtgtgga tatgtgtttc aatgtgaaaa ttgtgatata tctttaactt atcataagaa
    12601 aagcaatact ggaagatgcc actattgtgg atatgagaaa gaaattccaa aagagtgtcc
    12661 tgaatgtaaa agtacttatg ttaagccatt tggagttgga actcaaaaaa ttgaagagga
    12721 attaaaatat atttttccag atataaaaac tttgagaatg gataaagata caacttcaaa
    12781 aaaaggtgct ttagatgaga tactaaataa atttaaagat aaagaagcag atgtacttat
    12841 tggaacacag atgttaagta aagggctgga ttttgagaat gtaactttag taggtatttt
    12901 gtctgcagac atgatattaa attttccaga ctttaaaagt tttgaaacta catttcaatt
    12961 gataactcaa gtctctggaa gagcagggag agctgataaa gagggaaaag tagtacttca
    13021 gacttatgat actgaacact atgctattaa gcatgctatt gaatatgatt atgagggttt
    13081 ttatgaagat gagataaaaa taagaaaagc atttggatat tctcctttta ataatatgtt
    13141 aagtgtagtt gttagtggtg aagatgaaag attagtgata aaaaatataa aaaatatgca
    13201 tgcatcattg atttatttat tagaaaaaag gggtataaat gatttaggat ttatattagg
    13261 tccaaatcca tgttcaatat caaaaataaa tcaaaattat agatggcaaa tactctttaa
    13321 agatgaaaat attgaaatta atctcttaaa gggtataata aaatatatat gtataacaaa
    13381 acgagatttg atatttgata agaatatcaa tgtatctata gatattaatc caaacagtgt
    13441 attataaatt taggaggaat tataatggct ttaagacaaa tagttcaaat aggagaacca
    13501 gtattaagaa aaaaatcaaa aaaggttgaa aaaattgatg aaaagataat acaattatta
    13561 gatgatatgg cagaaactat gtatgatgca gatggtgttg gactggctgc acctcaagtt
    13621 ggaatattaa aaagagtagt agtaatagat attggagaag agcttataga acttataaat
    13681 cctgaaataa tagaaacatc tggggagcaa atagatgagg aaggatgttt aagtgttgtt
    13741 ggagaagcgg gaaatgttag aagaccaaat tatgtaaagg tcagagcttt aaatagaaat
    13801 ggtgaaacaa tagagttaga gggagaagaa cttttagcta gagcattttg tcatgagata
    13861 gaccacttag atggaatttt atttgttgac aaaatagaaa agtagtagga gttgatttga
    13921 tgaaaatagt atttatggga actcctgata tagcagttcc atgtttacaa aaaataatag
    13981 atgagaagta tgaaatattg ggtgttgtaa ctcaaccaga taaaccaaaa ggaagaggta
    14041 aaaaactagg tatgagtcca gtaaaagaac tggcaattga aaataatata cctgtatatc
    14101 aaccagtaaa ggctagagat aaagaattta tagataaaat taaatcttta aatccagatg
    14161 taatagtagt tgtagctttt ggacagatac ttccaaaaga aatattagag attcctaaac
    14221 ttggatgtat aaatgttcat gtttctttac ttccaaaata tagaggtgcg gcacctataa
    14281 attgggtaat aataaatggt gaagaaaaga ctggtgttac aactatgtat atggatgaag
    14341 gattagatac tggagatatg atactaaaga cagaagtaaa tcttgatgaa aatataacag
    14401 caggagaact tcatgataaa atgatgaata taggagcaga aactttaaaa gaaacattaa
    14461 gattaattga agaaggaaat gctccaagag aagtacaaaa tcatgaagaa ttttcctatg
    14521 cgccaataat gaataaatca ttaggtaaca tagatttttc aaaaagtgcc agagaaatac
    14581 acaatttagt tagaggagta aatccatggc caagtgctta tactacctat aatgatgtca
    14641 taatgaaagt ttggaaaact aaagtgttag atgaaaaaag cacaaaagat gtaggtacaa
    14701 taattgatgt aagcaaagat gggataaaag taagtactat agataatgta cttttaattg
    14761 aagaaattca gatgccgaat aaaaagagaa tgttggttgg tgaatatata aaagggaata
    14821 ctatagaaac tggtttagta ttgggttagg tgaaaagtat gacagatata aagaagtatt
    14881 taacaagtgt tgggattttg attttaatat taggaatttt gataggagtt gttaattttt
    14941 ttataataag tttaactcaa atattctcct tgatgataaa tattataata atatcactaa
    15001 tacttataat actatgttca acagcagtta cttatagagt aattaaagga aagtatgtta
    15061 actcaaatat aatgaaaata aattttaata ttgtcagtgg attatttcct attatatctt
    15121 tcattgcatc atcatttggt atgtcaaaaa gtgatattag aagaatatat ataaagttaa
    15181 ataatgaata tatatacagt aataaatata attttaatcc agaggatata ataattttaa
    15241 tacctcattg tattcaagag aatagttgta aattaaaagt tactaatgat atagataatt
    15301 gtaaagaatg tggaagatgc aacataggag aattaattaa gctaaaggaa aaaactaatg
    15361 taaaaatatt tgtagctaca ggaggaaccc tagctagaaa aattataatg gacactaaac
    15421 ctaaagctgt tgtagctgtt gcatgtgaaa gagatttgac atctggaata caagatatta
    15481 aaaaaattcc tgttttaggt gtatttaaca aaagacctaa tggaccttgt gtaaatacta
    15541 atgtagacat gatggatatt gaaaaagcta taggcttttt aacaggaaaa aatatattag
    15601 tttgtaatta aactgttttt aagttaagtg aatattaaga ggatgtataa taaaaatata
    15661 ttttaggaga gtgttattta tgtaccctgt atatggcggt ttttggggat ttgacccaac
    15721 aatggttgta ttaattccag ctatattact tactatatat gcacaattta aagtgagttc
    15781 aacaacaaat aagtatttaa gagtaaatac acgtcgagga tatactggag agcaaacagc
    15841 aagaagagta cttgattcaa atggtttgta tgatgtaaag atagaaatgg ttagagggca
    15901 tctaagtgac cattatgacc caagaagaaa ggctgtaagg ttgtcagaag atgtatatta
    15961 tggaacatct ataacttctg ttgcagtagc tgcacatgag tgtggtcatg ccatacaaca
    16021 tgctaaaggt tatgcacctc ttcaaataag aagtagttta gtaccagtag tgaattttgc
    16081 ttcaagtata tcttggtttt taatattttt aggatttatt atggctggtc catttttaaa
    16141 aattggtata ctattgtttt cggcttcagt gttattccaa ataataacat tgcctgtaga
    16201 atttaatgct tcaagtagag caatcgttca acttgggaat ttagggatta tagatgaaag
    16261 tgagtcaaga caaagtagaa gagtactttc agcagcagct ttaacatatg ttgcagcagc
    16321 attggtttca atacttcaat tacttagatt gttactaata gctcaaagaa gaaatgacta
    16381 aaagtaaagt gggttgttta gaaacaaccc ttttttatgt ttaataaaag ggagaaattt
    16441 atatggatgc aagagaagtt ggatttaaag tattatgtga tatagaaaaa aatgacaatt
    16501 attcaaatat tgcaataaat aagcatttta aaaatttaga aataagtgat atggatagag
    16561 gacttgcaac agagttaatt tatggagtaa tagaaaataa atattattta gattatataa
    16621 tcaataaact ttcaaaaata aaggtaaaaa aaatgtcaac ttatgttaaa atctttttaa
    16681 gaatgggaac atatcagatt ttatttttag atagtatatc agattatgca gcagttaatg
    16741 aaactgtaaa attatctaag aaatatgata aaaaatcttc tggtttcata aatgctattc
    16801 ttagaaatga aataagagct aaagagacta taatggacat aactgaggaa gatagtgtaa
    16861 aatacttatc tataaagtat tcctataact cttggattat aaaaaactgg atagataaat
    16921 ttggacaaga atttgctgaa gatttgttgg aagcaaataa tgaaaagcct agtatctata
    16981 taagaacgaa tacacttaaa ataagtagag aagaacttat tgaaaaatta agtgaaatgg
    17041 gtattgtatg tttaaaagtt cctatggtag aagaagctat taaagtcgaa aaaatgaaaa
    17101 atatagaaaa aaatgaatta tttaaggctg gtttatttac aattcaagat ataagttcta
    17161 tgatagtagg gaaagtaatt aatccaaaag aaggttcatt tgttttggat gtatgtagtg
    17221 ctcctggagg gaaatctact cacttagcta ctttaatgaa caatactggt caagtagtag
    17281 ctagagatat ttttgagcat aaactaaagc ttataaactc tactgtaaat agattggggc
    17341 ttaaaaatgt gtttgtagaa agatttgatg cttctgagat tgatgaaaat agtataagta
    17401 aatttgatta tgttttagca gatgttcctt gctctggttt tggtataata agacgtaaac
    17461 cagaaattaa gtataagaaa gaagaagagt taaaagacat cacttctata caaaagaaaa
    17521 ttttagaaaa tgcatctaaa tatgtcaaag ttggtggaac tttagtatat agtacatgta
    17581 caattcaaga tgtggagaat ataaatatag ttacttcttt tgttgaagaa aatcacaact
    17641 ttgaatttgt tccaattgat acagtaaatg tagacttaga caatcaagat aaaggatatt
    17701 taaagatata tcctaatatt catggtatag atggtttttt catagcaaaa ttaaagagaa
    17761 taaggtagtg agagaatgga aaagaaaaag atagtattaa aaaattttac tgaagatgaa
    17821 cttaaagaat ttatgaaaac tatagatgaa aagcccttta gaggaagtca aatattctct
    17881 tggatatata agggtgcaaa aacttttgat gatatgaaca atataccaaa aagtttgaga
    17941 aataaattag aagaggtttc atgtataggg catatagata tagaattaaa gttagaatct
    18001 aaggtagata atacaaaaaa atatttgttt ttattagatg atggaaatat aatagaaact
    18061 gtgatgatgg attatgatag tagagttact gtctgtgttt ctaaccaagt aggttgtagg
    18121 atgggatgta atttttgtgc atctacaatg gatggattaa ttagaaactt agagccatgg
    18181 gaaattttag accaagttat taaaattcaa gaagatactg gtaagagggt atcaaatctt
    18241 gtattgatgg ggagtggtga gccacttgat aactttgaaa atacaaaaca atttttaaaa
    18301 ataattaacg aaaaaaatgg tcttaatata gggtatagac atataactct ttcaacttgt
    18361 ggaatggtac ctaaaatgta tgagttggca gacttagaaa tagctataaa tttagcttta
    18421 tcacttcatt caccatatga tgaagaacga agaaaaatta tgcctgtagc aaatgcttat
    18481 tctattgaag aaatattgaa tgcatgtaga tattatataa aaaagacaaa tagaagggtt
    18541 acatttgaat attctcttat aaaaggggta aatgactcag aaaaagaagc aaaggcatta
    18601 gctaagctcc taaaaggtat gctatgtcat gtaaatttaa ttccaataaa taaagttgaa
    18661 gaaagagaat acgaaaaacc agataaagca tttatttata aatttaggga tagtttggag
    18721 aagaacaata tacctgccac agttaggatg tctatgggtt ctgatattag tggagcttgt
    18781 gggcaattga gaagaaaata caagtaattt gtaaggagga agtcgtatgg tttatagttg
    18841 tgcctcccat ataggaaaaa taagaaaaaa caatgaagac tattgtgagg gagaagttat
    18901 agatacagaa catggtccaa taggaatatt tgccatagct gatggaatgg gtggacataa
    18961 aaaaggtgaa gtcgccagta aattagcagt agaaaatata attgattttc taaaggaaaa
    19021 cttattacaa catgataatg taaaaataga ctatatagat gatatattaa aacaggctta
    19081 taataatgta aactcaattg tgcacaaaaa atctatggaa gatatagaat ttgaaggaat
    19141 gggaactaca ttagttacgg ctatagtgta taataatgtc ttatatgtgg caaatgttgg
    19201 agatagcaga tgttatttgt taacagatga aaaatttgat aaaataacta tagaccattc
    19261 cgtggttgaa gaacttatga tggcccatgt cattacagaa gaagaggcaa gaagacatcc
    19321 acagaggaat agaataacta gagcaattgg aacagatgat atggttgttg tagatatttt
    19381 taagaaagaa atcaaaaaaa gtgatataat acttcttgcg acagatggat taactgggtt
    19441 tattgatgat gaagatataa gagatttaat attagattat gaatatgaga gaactagtaa
    19501 tataagtgaa gaattgataa gtatggccaa tgatgtttct ggtaaagata atgtttcggt
    19561 tatagtgata aaagtataat taggacggtg atattgtggg agatacaatt ttaggaaatc
    19621 gatatgaaat catcaggaag attggtgatg gaggaatggc ctttgtatat gaggctaaag
    19681 acagattatt aaacaggact gttgcactta aagtacttag acctgaattt gtagatgatg
    19741 atgaatttct aacaaagttt aaaagagagg cagaagcagt agcaagtctt tcacatccaa
    19801 atatagtaaa tgtttatgat gttggtgaag atggaaaagt tcactacatt gtaatggagt
    19861 ttgtagatgg gaagaatcta aaagagatta tacaagatga aggtatatta gatgaatata
    19921 ctgcattaga tataacaaaa caaatagcta tggcacttag tgcagcgcac aaaaagggga
    19981 ttatacatag agatataaaa cctcataata ttctcatatc aaatgaagga agagtagtaa
    20041 aagtggctga ttttggtata gccaaagctg tttcaaattc aacaatgact aatattggta
    20101 gtataatagg ttcagtgcat tatttttcac cagagcaagc taaaggtaaa tttgtaacta
    20161 ataatgctga cctgtattct ctaggtatag ttttatatga aatgttgata ggaaaagttc
    20221 catttagggg agatagtcca atttctatag cacttcaaca cattaatgat gatatagact
    20281 ttacatcaga agaaaaagtt aggattcctc aaagtgtaag aactacaata aaaaagctta
    20341 cagaaaaatc tagtgcagat agatatcaaa ctgctgaaga attgatagaa gatatagagt
    20401 atatagaaaa aaatattgat ttagatttta taaaagaata tgatgatttt gcaactaaga
    20461 aaattgatga aaaagaaatt aataaagttg taaatccaac acttgcaaaa ccagctcctg
    20521 aaaaagtggt taagcctgtt gaagtagctg atttggatga tgatgaagat tactatgatg
    20581 atttttatga agaagatgat gatgaagatg aagaagagga agagataatg agagctaaaa
    20641 agaatcagag acctaagagt actccaagta agagaactaa gaaaaagaag aaaaaacagg
    20701 aaagcccaaa atctagaaga agattaaaag taattgcggc tgttttgata ctgatattgt
    20761 gtgctcaagt attcttagca tataagtttt tatttgctgg agggtttggc aataaatctt
    20821 tgactgttcc taatcttgta aatatgacat tagaagaagc tcaaagtgct gttgaaaaag
    20881 aaggattata cctgagtgta aaaagtgaag agtataatag tgaagttgat gaaaattgta
    20941 taatatcaca gacacctgaa ggtggaagta caaatgtcaa aaagggtgat actataaatg
    21001 ttgtcgtaag taaaggttca agtcaggcat ctgttcctaa tgtagtggga cttactttga
    21061 gtaatgcaaa acaacttatt gaagaaaata acttaaaagt gggaactgtt aagtatgaat
    21121 acagctcaat atataaagaa ggtacagttt taagtcagag tccgggtgct ggctcatcaa
    21181 gagctcagga aggtgatgag gtaaacctat atgtaagcaa aggttcagaa aaatcaaata
    21241 cacagactcc aacagtacca gataaaaaac caacaacacc agagaataat acaccaacag
    21301 aacctggttc aaactcagga aacagtggtg gaaattcagg aggctcaaat tcaggaaata
    21361 gtggtggaaa ctcaggcaat aatggaagta actcaggagg ttcaaactca ggaaatagtg
    21421 gtggaaactc aggaggctca aactcaggag ataatggagg aagttcagga ggctcaaact
    21481 caggagacaa tggaggaagt tcaggaggtt caaactcagg agacaatgga gatagttcag
    21541 gaggctcaaa ctcaggagat aatggaggaa cttcatcagg aactggcgca aacattggta
    21601 aaacagaata aatattaata aaataagagc tgtcctttta tagaaaatat tttctttaaa
    21661 gggcagtttg ttttgtataa aatactgaat tggttataat ataaggagaa aagtatttat
    21721 gaagaagaaa acagttttaa ttacaggagg agctagaggg attggtaaag ctatgtctaa
    21781 agcttttgca aaagaaggtt acaatgtatt agtaaatttt aataagtcag aaaatgaagc
    21841 taaagagttg tacactatat taaatgaaaa aaatttttct gtaaaacttt ttaaggctga
    21901 tatttcaaat agagaagatg tagaggatat ggtagattat tgtataaaag aatttggagg
    21961 attggatgta cttgtaaaca atgctggtgt aagtcaagat aagttgttta cagatataac
    22021 tgatgaagat tgggataata tgatgaatat taatttaaaa ggaagctttt attgttcaca
    22081 agttgcttta aaatacatga tatcagaaaa aaaaggaaat ataattaata tatcttcaat
    22141 ctggggtata tcaggtgcgt cttgtgaagt gcattattct ataacaaaag caggcataat
    22201 aggtatgaca aaggcattag ctaaagaagt aggaccatca aatattagag taaatagcat
    22261 agctccaggt gtcattaata ctgacatgtt atctggatat aatgaggaag atatagacgc
    22321 tcttgtggaa gaaacccctc taatgaggct aggaactcca gaagatatag caaattgtgc
    22381 tattttcttg gcatcagaca aatcaaattt tatcactggt caggtaatta gtccaaacgg
    22441 aggatttgtt atttaaaaaa aatgtggtat tatataaaaa taggaaagaa taacacgata
    22501 tactactcat aaacagtatg tgtttatata aattttataa agggaggcat ttgatgctag
    22561 aaggaaaaat tataaagggg attagtggat tttattatgt agatacttat aatggtattt
    22621 atgaatgtaa ggctagaggg atatttagaa agcagaaaat aacacctttg gttggtgata
    22681 gagtcaaaat aagcattgtt gatgaaaatg aaaaaaaggg tatcttagaa gaaatcgaca
    22741 gtagagatac agaacttata agacctccta ttgcaaatgt agataaagca ttaatagtat
    22801 tcgcaataaa aaatcctaaa ccaaacttat ctcttttaga tagatttata gttttggcag
    22861 aaaaagaaaa tttagaaact gtaataatcc ttacaaaagc agatttagat gataatgata
    22921 cattagaaac tgttaaaaat atatatgaac ttagtggcta taaagtcatt cctgtaagca
    22981 atattactaa gttaaatata gataaagtaa aagaagaatt aaaagaaaat gtagtagtgt
    23041 ttgcaggacc atctggagtt ggaaaatcaa gcttattaaa tgaaatagat gaaaatttta
    23101 aacttcaaac aggagttgta agtgataaaa taaaaagggg aaaacatact actcgtcacg
    23161 ctgaactttt aaaattagaa tttggtggaa tggttgcaga tacaccaggt tttagttccc
    23221 ttgcccttga agatatagag gaagttgaac ttaaagatta ttttatagaa tttgataaat
    23281 ttaatgattg taagtttggt tcaaagtgta ttcatgaaaa tgaacctaat tgtgctataa
    23341 aagaagctgt tacaaatgga gaaatatcta aagaaagata tgatagttat atacagctgc
    23401 tacatgaaat aagacaaaat aatagtagga ggtattagaa acatgattaa attagcacca
    23461 tcaatattat cagcagattt tgcaaaatta ttagaagatg taagaaaagt agaaagtgca
    23521 ggatgtgaat atcttcatat cgatgtaatg gatggacact ttgtaccaaa cattacatta
    23581 ggaccacttg tagtcaaaag tttaaagaaa gaaaatataa atatggtatt tgatgctcat
    23641 cttatgatag aaaatccaga ccagtatatt gaagaatttg taaaagcagg atgtgatata
    23701 ataactgttc accaggaggc ttgtgttcat ctacatagaa ctatacaaaa tataaaatca
    23761 catggaataa aagctggtgt agtacttaat ccagctacac cagtagatac gattaaacat
    23821 gtactacctg atttagatat ggtattatta atgtctgtta accctggatt tggaggacaa
    23881 tcatttattc cttgtgtttt agataagatt aaagagttaa aagctattat agatagtcaa
    23941 ggattaaaca ttgatataga agttgatggt ggtataagcc ctaaaaacgt agccgaagtt
    24001 gtacaagcag gagctaatgt aatagtagca ggttctgcaa tatttggaag tgatgacatt
    24061 caagaaacag ttaatttatt tagaaagaat gcttctcttg aagaattagt ttaaatttaa
    24121 taatgaaaat ctgtattgtt ttaaatggcg aaattgaaga ttacgaagtt acaagagata
    24181 ttataattaa ggaatgttat gactgcataa tatgtgcaga tggaggggca aatcacactt
    24241 ataaaatgga aataatgcca gattatatat taggagattt agattcagta gaagaagaaa
    24301 agataaactt ctataaaaat aaaggtgtta aatttgaaaa atttccatct aaaaaagatg
    24361 agactgatac agagctttgt ctttttttag ctaagacact taaagcaaat catattgatt
    24421 tttttggtgc tttaggggga aggattgacc acactcttgc aaatataaag ctattatatt
    24481 atttaaaaga agatggtata tattctagga tactttctga taaagaagag atgtatatag
    24541 ttgaaaatga ggaaatttca ttgtatggaa acccagggga cactatttct gtaatagcta
    24601 taaatggtga tgctaagggt gtaacactta ctggcttaga ataccctctc gataattatt
    24661 atatgaaata ctctgtacct attggaatat caaatgttat gttgagtaat tcttgtaaga
    24721 ttaaagtaga gcaaggctgt gtacttgtag ttagaaattt ataaaatttg attttgacaa
    24781 ttggttttat attgtagagc agatacaatc aagtaaaata tatttatgat tgtatctgct
    24841 ttttagtata taattataaa tatataataa actttgacat aagggggatt ttttgtggta
    24901 gatagtatta tttttgattt agatggaaca ttgtgggatt caactgaaga agtatgcaaa
    24961 gtctggcagg atgttttaag tgagcataag gaaatagaat taagtgtcac aaaagattta
    25021 tttagaagtc ttatggggct ttcttttgag gaaatagcaa atcgtttatt tccaaattta
    25081 agtgaagaag aaagaatgaa tttattgaat gaatgttctt tgaaagaatg tgactattta
    25141 tctgaaaatg gagctaaatt atttgatgat atagaagata ctcttaaatt actatctaaa
    25201 aaatataaat tatttatagt aagtaattgt caagctggat atatagaatc ttttttaaaa
    25261 gcacataaac tagaacagta ttttatagat tttgaatgtc ctggtaatac tggtttgcat
    25321 aaaggagaaa ataataaact tataattgaa agaaataaat taataaatcc aatctatgtt
    25381 ggtgatacgc aaggtgatgc aaatagtgct aagtttgtag gtataccttt tatatatgcc
    25441 aaatatgggt ttgggaatgt agatgtatat gattatttta tagattcatt taaagatttg
    25501 ttagaacatg atatattaaa ataagatgag tttaaaaatt atattaaaat aataaaaaag
    25561 ttatgggggg atttaaaaat ggaattacaa gatactattt ttaaaagaca aagtgtaaga
    25621 aagtttaaaa atcaagatgt atcagatgaa gatattttaa aaatgataaa agcagcaggt
    25681 gcagcaccat caggcaagaa tattcaaaac tggcattttg ttgttataaa acgccgtgat
    25741 ttaatggaaa agatagcaga tgtgataact aaaaagcaac aagaaatact tgtagaaatg
    25801 gataaagtat cagtggataa ggctaataga tttagaaaat ttgttaaaaa ttttacacta
    25861 ttttacttaa aagctccagt tttagtatta gtttttacaa aggtatataa tccatcagga
    25921 tattatgaat tagagcttat agatgcacct aaggagacta tagataaatt atttataaga
    25981 aatccaggta tgcagagtct aggtgctgca attgaaaact ttacactatc agcaattgaa
    26041 cttggatatg gttcttgttg gttgacaagt caaaactatg cagcagatga gatagaagct
    26101 gttttagaag cagaaactgg ttttgaaaag ggagagtatt ttttaggtgc aatgttagct
    26161 cttggagtgc cagaggacaa tttgaagagt ccatctaaaa aaccagtaga agaaatatgt
    26221 acatttataa aataaaagtc aaggacattt taaaatcgaa gtaaattaaa aacaaaataa
    26281 attaaaaata
//
LOCUS       DAEORZ010000044        26002 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEORZ010000044 DAEORZ010000000
VERSION     DAEORZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26002
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.044"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(179..784)
                     /locus_tag="KRH39_001677"
     CDS             complement(179..784)
                     /locus_tag="KRH39_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG8511590.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(909..1026)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1086..2252)
                     /locus_tag="KRH39_001678"
     CDS             complement(1086..2252)
                     /locus_tag="KRH39_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8511591.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(2400..4541)
                     /locus_tag="KRH39_001679"
     CDS             complement(2400..4541)
                     /locus_tag="KRH39_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBG8511592.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNISEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(4685..5792)
                     /gene="prfB"
                     /locus_tag="KRH39_001680"
     CDS             complement(join(4685..5716,5718..5792))
                     /gene="prfB"
                     /locus_tag="KRH39_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBG8511593.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(5807..8482)
                     /gene="secA"
                     /locus_tag="KRH39_001681"
     CDS             complement(5807..8482)
                     /gene="secA"
                     /locus_tag="KRH39_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG8511594.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(8614..9165)
                     /gene="raiA"
                     /locus_tag="KRH39_001682"
     CDS             complement(8614..9165)
                     /gene="raiA"
                     /locus_tag="KRH39_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG8511595.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(9718..10392)
                     /locus_tag="KRH39_001683"
     CDS             complement(9718..10392)
                     /locus_tag="KRH39_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBG8511596.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(10395..11447)
                     /locus_tag="KRH39_001684"
     CDS             complement(10395..11447)
                     /locus_tag="KRH39_001684"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBG8511597.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(11496..12455)
                     /locus_tag="KRH39_001685"
     CDS             complement(11496..12455)
                     /locus_tag="KRH39_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBG8511598.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(12551..13069)
                     /locus_tag="KRH39_001686"
     CDS             complement(12551..13069)
                     /locus_tag="KRH39_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBG8511599.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            complement(13080..14414)
                     /gene="celB"
                     /locus_tag="KRH39_001687"
     CDS             complement(13080..14414)
                     /gene="celB"
                     /locus_tag="KRH39_001687"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBG8511600.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(14453..14779)
                     /locus_tag="KRH39_001688"
     CDS             complement(14453..14779)
                     /locus_tag="KRH39_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8511601.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(14802..15110)
                     /locus_tag="KRH39_001689"
     CDS             complement(14802..15110)
                     /locus_tag="KRH39_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG8511602.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(15251..17164)
                     /locus_tag="KRH39_001690"
     CDS             complement(15251..17164)
                     /locus_tag="KRH39_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8511603.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(17496..18278)
                     /locus_tag="KRH39_001691"
     CDS             complement(17496..18278)
                     /locus_tag="KRH39_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBG8511604.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(18281..20500)
                     /locus_tag="KRH39_001692"
     CDS             complement(18281..20500)
                     /locus_tag="KRH39_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBG8511605.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMILVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(20764..21861)
                     /locus_tag="KRH39_001693"
     CDS             complement(20764..21861)
                     /locus_tag="KRH39_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511606.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(22177..23370)
                     /locus_tag="KRH39_001694"
     CDS             complement(22177..23370)
                     /locus_tag="KRH39_001694"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBG8511607.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(23507..23610)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            23799..24308
                     /gene="yyaC"
                     /locus_tag="KRH39_001695"
     CDS             23799..24308
                     /gene="yyaC"
                     /locus_tag="KRH39_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBG8511608.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(24419..24847)
                     /gene="fabZ"
                     /locus_tag="KRH39_001696"
     CDS             complement(24419..24847)
                     /gene="fabZ"
                     /locus_tag="KRH39_001696"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBG8511609.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(24863..25882)
                     /locus_tag="KRH39_001697"
     CDS             complement(24863..25882)
                     /locus_tag="KRH39_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG8511610.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
BASE COUNT         7788 a         4893 c         2743 g        10578 t
ORIGIN      
        1 ttttaattct tcctttattt taaaatttaa atttgtctaa atatttctta ttttatatac
       61 attttttgat aaattaataa agccataata tatttaatat atcaatcaca atataggact
      121 gaagttataa ataaattatg gctattcaat acatttaatt ttatattttc ttgtctaatt
      181 atttctttat tatatttccc atttttttat aaagtggcaa tgttactaaa gatattataa
      241 cagccttcag cacattaaat ggagctatca tccaaattac aaaagtgaat aaatcatgaa
      301 cttgaggatt tatagctgaa cctaaaccta ttatagcatc tagtcccata agttgaccat
      361 aaagtggcaa cattacaaaa taatttacta atgaaccaat tatagtcata gatattgttc
      421 ctaatacaaa accaatcagt acacctttta aatctccact tctcttatat gaataacaaa
      481 gtatcattac ataagaacca ccaattaata tatttgcaaa ttctcctatt cctccagttg
      541 ttgttgctgt tattgcttgt aacacatttt ttactacaac tattccaaat ccaactaatg
      601 gtcctagaga cattcctccg aatattgctg gaatatctga taagtcaatt tttaagaaat
      661 ctggaaatat acctggtatt ggtgcttgaa taaacattaa taaataagat attgcagata
      721 aaattgacat tactactaaa gttcttgttg aaatcatatt gcctcttttt gctgtgtttt
      781 gcataattat tttcccccta atcaatatgt ttatattttt taggaaaaac aaaaaactcg
      841 aagaccctat cttcgagttt ttatggatat agtttaaaca aactaaaata aacttattta
      901 tcttccataa atatcttctc tcatccagac tttactgtcg gctttggaat ctcaccaaat
      961 caaccaccta agtagttcgc gggctatacc gccggtaggg aatttcaccc tgccccgaag
     1021 atcttctttg attttttaat tttccttaca ctcttatatt agttaatttt ttaataattg
     1081 tcaagttatt ttttctcata cactactttg ccatttatta tagtgtaaag tacattactt
     1141 tgaatctcta gtggagaatt atcccaaatt actaggtctg catctttacc aacttctata
     1201 gagcctactc tatcttctat tcctaaagtc ttagctggat ttattgtaat tgattctatt
     1261 gctttttctt ctttcatacc atgttttact gcaatacctg cacatattgg aagatattga
     1321 actggtatta ctggatggtc tgtcattata catacatcaa gacctgcatt tgaaagtatt
     1381 cctgctgtat taaatgtcaa atttctaagc tcaaatttag acctttctga caaagatggc
     1441 cctactatta caggaaaccc ttcttccact aattcatcta caataagatg accttctgta
     1501 caatggtcta atgtaagctt taaattaaat tcttttgcaa ttcttattgc tgtaaacata
     1561 tcatctgctc tatgagcatg tgccttaaaa ggaatttctc ttcttaaaac tggtattaaa
     1621 ctttccattt ttatatcata ttcaggtttt tcacagtctt catcatcatg actctcatac
     1681 atatcaatat cctctaaata ttcttctgct ttttttaggt tttctctaag taaagcagct
     1741 atagccattc tagtttgagg agatttatca tcctgaccat aacagctctt tggattttct
     1801 ccaaaagcaa ttttagatgc aactggattt tttattacca ttttatcaat tctttttcca
     1861 catgtcttta tagctataca ttgaccaccc ataacatttg cacttccagg ggttgtacaa
     1921 acagaagtta ttccaccttc aaaagcttct ttaaaagttc tatccattgg attaattcca
     1981 tctataggat ttaattgtgg tgttattggg tcagtctctt catttccatc tgctccttca
     2041 aaccctatac catcttccca taagcctaag tgagtatgag catctataaa tccaggaaac
     2101 acaagtttat tactagcatc tattacctct acatctaaag gagctatcaa atcttctcca
     2161 atttctatta tttttccttc atctataagt atatctccat gaattatccc atttgttatg
     2221 gtatttattt taccattttt tataaaaatc attcattttc ctcctgattt tcaagactat
     2281 cgttcttatt ttaatatatt ttcttactat atacttttac aaatttttaa catttataca
     2341 ctcgagtaat ctcatttaca aatatagaat ctaagtacta taaccataca taaacttatc
     2401 tattttttca ttgataaggc aactcttttt ttgtttaagt caattccaat gacttttaca
     2461 tctactatat cacctactgt aacaatactc atagggtctt tcacaaaact attactcatt
     2521 tctgatttat gaactaatcc gtcatttttt attccaatat ctacaaatgc accaaagtct
     2581 acaacatttc ttattgtacc ttttaaagtc ataccctctt gtatatcttc aatttttaag
     2641 acatcagttc taagtatagg ttttatacct tcttctcttg ggtctcttcc tggttttttg
     2701 atttcagcta taatatcttt taatgtaact tgtcctactt ccagtttttc acttaattcc
     2761 ctcaagccaa tttcttttat tttttcatct atttcactta tattcttatt ctttacatca
     2821 tctaaagaat atccaattat ctcaatcatt tttttacata tatcataact ttcaggatgc
     2881 acacctgtat tatctagtgg atttttaccc tctaagattc tcataaatcc agcacattga
     2941 gtaaatgctt gaggtcctag tctttttact tttttcaatt gtgctcttga cgtaaaatct
     3001 ccattttctt ctctatatgc tattatattt tttgctatag ctttacttat tcctgcaaca
     3061 tgttctagta gggagtatga tgcagtattt aaatctactc caacactatt tacagaatct
     3121 tcaacaactc catctagtac ttcttctaat ctctttttgt ttaaatcatg ctgatattga
     3181 ccaacaccaa tacttttagg gtctattttt acaagctctg ctaatgggtc ttgtaatctt
     3241 ctagctatag atatagctcc tcttattgat acatttatat ctggatgttc ctcatttgca
     3301 agttcagatg ctgaataaac agatgctcct gcttcactaa ctatcacata ctgcacttcg
     3361 ctatctattt cttttatcat ttctgataca aaagtttctg attctctaga tgcagtacca
     3421 tttccaattg aaattatatc tatatcgtat ttttcaatca atccttttaa aactttcttt
     3481 gctccctcta catcattttg agggtctgtt ggatatactg ttgtataatc tagtagttta
     3541 ccatttttat ctactacagc tattttacat ccagttctaa atgcagggtc aaatcccata
     3601 actactttgt ctttaactgg tggttgaagc aataaacttt taacattctt tccaaataca
     3661 cttatagctc tttcttgagc tatttctgtt aaatgatttc ttatttctct ttctattgat
     3721 gggaaaatta atcttttata agaatcttct attgaactta cgatttcttc tttatttttg
     3781 aaatttttat catttacata ttcgtttatt atgtaattta aaactttatc attatttatt
     3841 tccaacttaa cctttaggaa gctttctttt tctcctctgt ttattgccaa aactctatgt
     3901 ggagccatac tttttactgc ttcgctataa tcataataca tatcataaac cgatttctca
     3961 tcagtagcac ttttgctaac tatcatacct tctcttaaag ctaattctct tatatacttt
     4021 ctaatcttag catcatctga gactagttct gcaattatat ctctagcacc ttttagagca
     4081 tcttcaatag aaagaacttc tttctcttca tcaagataat tctttgcctc tatttctata
     4141 ttatctaaat tattctctag tattgacaat gctaaatttt ctaatccttt ttcttttgct
     4201 attgttgctc tagttctttt cttttgttta tatggagcat atatatcttc tacttcttgt
     4261 aatgtttttg ctttttctat attttgtgtt atttcatctg ttaatttccc ttgttcatct
     4321 attagtctaa ctacatcatc ttttctactt tgaagatttc tcaaatacat taatttttca
     4381 taaaattctc ttagtgtaac atcactcatt tcgccagtca tttcttttct atatctagct
     4441 ataaatggta ttgtatttcc ttcatcaata agttttaatg tattatttat ttgttcatct
     4501 ctaagattaa actctttttt taagatttga tttatatcca atttattcac tcctaatgtt
     4561 agtttttcat aagtataaca aaacagaacg aaggctgagg gttatcgtcc tcagcctaag
     4621 tgctgtaagt gcatattaag cactaacctt cttatacata tatcttaatt acagtgcata
     4681 tttattaaac tattttattc atttttaagt attcattaat aaacaagtct atatttccat
     4741 tcattacact atctacattg ccaaactcag catttgttct atggtcttta accaatttat
     4801 atggttgaaa aacatatgac ctaatttgac taccccaagt tatttgtgag tatttgcctt
     4861 gtatatcttc tattttttcc ttttgctcta actcttttaa ttctatcaat ttcgccatta
     4921 aaagtctcat agctctgtcc ttatttagat gctgagacct ttcattttgg cactgaacta
     4981 ctacaccagt agggatatgt gttattctaa ctgctgaatc agtcgtattt acatgttgcc
     5041 ctccagctcc agaagctcta tatgtgtcta tctttaaatc tgatggatta atttctactt
     5101 ctatattttc atctaactct ggtgttacat ctatagatgc aaatgaagta tgtctttttc
     5161 ctgatgggtc aaatggagat attctcacta atctatgaac tcctttttca ctctttaaat
     5221 acccataagc atttgttcct tctacaagaa ttgtagctgt ctttatccct gcttcagggt
     5281 ctgatattat atctaaaagc ttaactttat aacccttact ttctgaccat cttatataca
     5341 ttctcaaaag catttgagcc caatcttgag catctaagcc accagtacca gcatttatag
     5401 ataatatagc attattttta tcatactctc catttagaag agtctttatt ttcatttcag
     5461 ataatttttc ttccattgat tctatggact tttctatatc tctttctaca gaatcatcat
     5521 cttcttctaa acctatttca attaatactt ctatatcttc taataagctt tttaagcttt
     5581 catattcttc taatgtctct tttaaagatt tattttcttg aagtactctt tgagcgactt
     5641 cattatcatt ccaaaagtct tgttgattca tcttttcttc attaatttgt atatttttca
     5701 tcaatgaagc tatgtcaaag agaaaccctc aattcttcta aatttgaagc catctgctcc
     5761 atactatgtc tatactcttg taactttaac atttaatcct ccgagattat cttccacaac
     5821 agtttttata ttttttacca cttccacaag ggcataagtc atttcttcca accttttcat
     5881 cttttttaac agttttactt gttggaagtg ttccatctga tggagaagat agattttcaa
     5941 catctacaac tgcttttctt tccactggag tttctattgt tatattaaat aaatatctta
     6001 cagtatcttc tttgatatgt ttattcattt catcaaacat atcaaaacct tccattttat
     6061 aagcaataac tgggtcttgc tgacctacag cacgaagacc aataccttgg cgtaattggt
     6121 ccatagcatc tatatggtct atccaatggt tatcaacagc ttgaagtaag attactctct
     6181 caacttctct cattctctca tagccaactt gttcttcttt tgaagtatat atcttcatag
     6241 ctatttcata aactttttct gttatttcaa ctggattaag tttttctatt tctggtattt
     6301 ctatgctacc ttttggcaag aacagattat acatatgttc tttgaatcct tcttcatcaa
     6361 atcctttatc ttgtgtataa agagtaactg cttcttctat tatagaatga gtcattgatt
     6421 gtatttgttc ttgtaaatct tcaccttcta atacacgttt tctctctgca tatattattt
     6481 ctctttgttt attcataaca tcatcatatt gaagtacatg ctttcttatt ccaaagtttt
     6541 taccttcgac tttcttttgt gctccttcaa tagacttaga taatattcta tgttctatcg
     6601 gcatgtcttc ttcaagacct attttatcta caataccaga tattctatca ctaccaaaca
     6661 atctcataag gtcatcatca agacctatat agaatcttga actccctggg tcaccttgac
     6721 gtccagcacg acctctcaac tggttatcta ttcttcgaga ttcgtgtctt tctgtaccta
     6781 ttatagcaag tccacctgct tctacaactt gtttttgttc ttcttgtgtt tgttgcttaa
     6841 acttttcaaa tagtttttca tactcttctc tagcttcaaa tagaatttca ttacctttta
     6901 caggtatacc ttctattgga gtatctactc tatttactat ttcttcttta aatccatttc
     6961 ttctcatttc tctcttagtt aaaaagtctg gatttccacc tagaactata tctgtaccac
     7021 gaccagccat attagtagct attgtaacag ctccaagtct accagcttgt gctattattt
     7081 ctgcctcttt atcatgatgc ttagcattta atacctcgtg ctttatgccc tttttcttta
     7141 gtatttgtga taataattct gatttttcta ttgatactgt acctacaagt attggttgat
     7201 tgactttgtg cctttcaatt atttcttgtg ctacagcatt aaatttacct atttcacttt
     7261 tgtatacaca gtctggtaag tcttccctta tcataagctt attagtaggt acttggaaaa
     7321 catccatttt gtagattgct ttaaactctt cttcttctgt ttttgctgta ccagtcatac
     7381 cagaaagttt tttgtacatt ctaaagtaat tttggaatgt tacagtagct agtgttttag
     7441 attctctttg tattttaaga ccttctttag cctctattgc ttgatgtaaa ccttctgaat
     7501 atcttctacc aaacataagt ctacctgtga actcatctac tattactatt tctccatctt
     7561 tagcaacata atcaacatct ttcttcatta ttacatgtgc tcttaaagct tgatttatat
     7621 gatggtataa ttctgtatga gatatatctg ttatattatc aacattaaaa tacacttctg
     7681 ctttttgtat acccgatgct gttagagata cagccttatc tttttcttct aattcataat
     7741 catctggttt taaggttaaa acaaatgtgt ttgcatctga atataagtga gttgatttgt
     7801 ctccaggtcc agatatgata agtggtgttc tagcctcatc tattagtata gagtcaacct
     7861 catccactat agcataattt aatcctcttt gaactctttg ctctttgtgt attaccatat
     7921 tgtcctttag atagtcaaat ccatattcat tatttgtacc ataagttata tcacaatcat
     7981 attgttcttt tctaactttt ggattttgac cgtgtataat aacgcctaca ctcattccta
     8041 aaaactcata gatttttgcc atttggtctc tatcacgttt tgcaaggtaa tcatttactg
     8101 ttacaacatg tacacctttt ccagtaagcg cattcaaata aacaggagca gttgcaacca
     8161 aagttttacc ttcacctgtt ttcatttctg ctattcttcc ttgatgtagt actatacctc
     8221 caatcatttg aactctgtag tgtctaagtc ctaacactct cttagaaact tctctaacta
     8281 ctgcaaaagc ttctggaagt atatcatcta tactttcacc atttgctaat ctttctttaa
     8341 atatatttgt catattttta agttccgagt ctgccataga ttcaaattta ggttctaatg
     8401 aatctattat atcaacagtt ttattgaact tttttaattc ttttttatct gccatgttga
     8461 ataaattatc cataaagctc atattttaca tctctcctcg ttctttattg tactattatt
     8521 cattttacca taaaaactat acttttaata ttaatttaaa aagctatcga taatttattt
     8581 atcgatagct aatatgatta aatctaaatt gctttattca tgttctatta taccatatcc
     8641 accattatta cgcttataaa ctactgctat ttcatctgaa tctatgttct tgaacatata
     8701 aaaatcatgt cctattaatt ccatctgtaa tacagcttct tcttcactca ttggcttaac
     8761 actaaatttc ttacgtcttt gaattactaa ttcttgattt tcatccccat aatcctcttg
     8821 ttcatcagct agatttatct ctgcatcttg gaatcttata ctcttgttgt ctttatgttt
     8881 atcttgtaat ctctttttat atttttttaa ttgtttactt aatttatcat atacaacatc
     8941 tatagctgtg tataaatttt cctgcatatc ttctgctcta attataggac catttacagt
     9001 agctattgtt acttctattt tttgtcttgc ttttctcgca cttactgtaa cttttacatc
     9061 agtgtctgga tgaatgtaat tatctaactt acttagttta gtttcgattt tattttttat
     9121 tgcatcagtt aattcgattt gttttccaga tataattatg ttcataaata catctccctt
     9181 caattatgcc agtataagaa aatttatact ttaataatat attctacacg aactctcata
     9241 atcctctttt tattatttac ttttatgatt ttttctattc aattgataga attatttatc
     9301 atttttagtt acattgatac ttatgtggat taatattatt acaaacacct gtggataatg
     9361 tggataaaac tgttaataac tcttgttttt gtttatttaa agctattttt actcctattt
     9421 ctatccacaa aaatctccat taattgttta ttaatatgtg aattcatgtc ataagatggt
     9481 aattttagga aattatgtgt attatatgcc aatatacctt tataattttt taataaatat
     9541 aaaccctcta aaacctccat aatctatctt ttctttcatt atttatattt aaaactactt
     9601 aaaagttata aagcatactc aacaataatt tcatatttaa tacctattaa cattcatata
     9661 cccaaattat aattaactta ctctctttat ttaaactaag tattttatat tcatatacta
     9721 tcttgaaatt atatttacga gttgtttaag cttttgtcta tctatatctt caaaaggact
     9781 gttatttatt ctaatcgctg taccaaaatg aaaattactt gccttagtca attcctttat
     9841 cttttctata ttattaaaat taagacctcc acctaaaaga atttttatgt gattagaact
     9901 aagcatcata ttctttatca tttgtatgtt atcaactata cttcctttcc ctcctgatgt
     9961 taatatattc gtaattttat cataatcctt caaaatttta acactatcta ttgtatttga
    10021 ttcatctatg gctctatgaa atgtaacatc aagattatca caacatttca ataaaacatt
    10081 tagatttttc tcatctatat tattgttttt atccaataca ccaaatacaa ctccattagc
    10141 accaatctct ttaaccacac ctatatcttt ttgcattata cttatatctt ctttactgta
    10201 tacaaaactt ttcgcatgat gtcttatcat tacgttcaca ggtatcttta cactgttaac
    10261 aacactttct ataagaccaa aacttggtgt caatccacct tcagttaaag cactaactaa
    10321 ctctattcta tctgcaccac aatcttctat aattttagcg tcttctactg acattcctat
    10381 tatttctaac ataactactc ctttgctaat tcatatattg catttgcaaa tatctttgca
    10441 tttaatatta tatcttctat atccatgtat tcatcaggat tatgagctat tcctttttgt
    10501 cctggaaatg aaggtccaaa cggaactata tttggcatta cttttgcata cgtacctcca
    10561 ttagttgtta cgggagttcc atctaatgat gtaacccttt cataagctag ctgaagcttt
    10621 ttaacaagat tactctcttt atcaaaatat acaggattaa aattagaaat tacctctata
    10681 tcgatactat ttggaaaact agcttttatt tcatctaata catctttcaa tttataacta
    10741 aaatcgtatt ttacctttat atctaatata gtatcactat tttctatatt cagctcttta
    10801 tatgtaacaa gcacattttc agacatattt tctataaaac ctaggtatac atcttcttca
    10861 taattgattt tactttttat tctaatcttt gccatgcctt tttcaccata tacaacagga
    10921 tacttacaat ctggtgtaaa tcccataata ggtgcttttt ctttttctaa ataatatggt
    10981 atatcttcaa atccactttc ttcatttgta ccaaatatta ttctaactct cttatctaat
    11041 tttaagttta attctttaat agcatataat ccatataagg cagacattat aggtccttta
    11101 ttgtctagta ctcctcgtcc ataaattctt ccattagttt cttctccctt ataaggctga
    11161 tgcttccaac catctccttc atgtacaaca tctacatgtc caattacaca aacataatca
    11221 tcactatctc catgctctgc ataaccaata taattatcca agtttctaac cttaaatccc
    11281 aatttttctg atatttctaa tgctttatta agtgcctccc ctactttttc gccaaaaggg
    11341 aagccatttt ctgactcccc tttgacacta ggtattttta caatatctat aatatcactt
    11401 aataaatctt cttttaactc atctatttta ttacttatga tattcataaa aatctcctta
    11461 ttataaaact ctattttaat tatgtaacca ctactttact cacttacttc tattggtgct
    11521 tttattctag cctcatatgc ttccatccac tcatttgata taaccttatg tatagtcttt
    11581 ccatctactc tatttgaaat atatacagta ggagattctt ctttagttac tactgaaaaa
    11641 gtaaaatcag ctatatttgt agctactccc cattcacctt tattattcat ggcaactaat
    11701 gataaatcac cagcttttcc ccttcttttg ataagctgtt tatcaagttc atttactgca
    11761 atatcacatg cttcttgtgg atgcttccct tctttcataa gtctaactat ctcataagat
    11821 atacatccct tcattaaatc ttctcccaaa ccagtagctg tagctcctcc tatactacta
    11881 tcaacataga atcctgaacc agatagtgca gaatctccta ttctacctct tttcttcata
    11941 aacaatccac ttgttgatgt agctgcacac atttttccag ttttgtctaa agatatcata
    12001 ccaacagtat catgaccagc atatggactt aatcctttgt ctaaagtctc tttttttctc
    12061 tttttataat gtaacttcgc tctatcagtc aacatatttt ttctttcaaa tccattttta
    12121 tgagcataat cttcagcacc tattccaact aaaaaattat ttaccttctc ataacttaac
    12181 ttcctagcta tgctgacagg attagaataa tctcttatac cagcaactgc gccaatatct
    12241 aatgtgttac catccataaa tgcagcgtca agttctacct cacaattttc atttggaagt
    12301 ccaccatatc caaccgattt atagaatgga tagtcttcaa ctcttcttac agcaagctct
    12361 acagcatcac ctgcttccat gcctttttct aaatgttctg atgcctcagt tattccttct
    12421 agggccattc tccatgtcgc tatcattccc cacatgttaa aattcccctc cttaaattat
    12481 atagatttta ctaatgtatg cttattataa atagtataat atttaaactt gctaataatt
    12541 aatgctctta ttaataacaa aattcttctt tcatcaaagt tatatcttct ttatttgcta
    12601 ttgcttttgc caatttacat atgttattta acgaatcatt aagaccaatt ccaccttttt
    12661 ttggagtctt aactatacat atgtcatttt tatattcgtt tatcacatct atattttctt
    12721 ctgacatagc tgcaaaagct ggtatatctg ttttatcatt tatattttta gacagtattg
    12781 ctgacatctt agaaaagttt tcgtaattaa agcttggtcc acagattact acatcagggt
    12841 ttaatttttt taccatagct atcatcttac tcgttacttc ttcttgatta tttacaaaaa
    12901 attcatcccc acaaaataag gtagctatta ccttagattc atttaaaaat tgctccatca
    12961 ttacacctgg tcctaatggg cttgattttc cagcaggagg tatattactt ttctcttttc
    13021 ctccagctcc agcttgtatt tggtccaata tcataactat tttcttcata atcttcctcc
    13081 tataatacta aatcatcaaa agatatatca tcatcttcct cttcaacagt ttcattttct
    13141 tctctcaagt attgcttatc tagcattttt ataaatggta tatacataaa tattcctagt
    13201 ataagaagta ctaattgtaa gactgcacct tgccatcctg ttgttaaaaa accagagaat
    13261 ataattggag ttgtccacgg taactgaact ccatttgtaa gaggcactaa tcctgctgac
    13321 atacagaagt aagctattat tatatttatt gttggaacta ttgcaaatgg tatcaatagt
    13381 attgggttaa gcattattgg tagaccaaat atcaatggct catttatacc aaatattcct
    13441 ggtaaaattg atagttttcc cattgacttt atacgtttac ttttacatat taataacata
    13501 gctatgacta atgatagtgt actacctgct ccaccaaatg tcgcaaacat atcttggaat
    13561 tgacctgtta ttatgtttgg tatttttgaa ccagcttgaa aagcagctaa gttttcagct
    13621 gatagaattt ttaatattgg attatacaca gccccaacaa cactacttcc atttattcca
    13681 aagaaccaga ataaatgtaa gaatatataa gcgattgcca tagcacctaa actatttcct
    13741 aattttaata atggcatttg taaaaactta aatataaatt catgtatact tccatatggt
    13801 gtagcttcaa aagctatctt aactaaaaag aaaactacca taactattgc acttggtatt
    13861 aatgctgcaa atgattttga tacagttgga ggaactccat ctggcatttt caatgtccat
    13921 ccttttttta caactgcagc aaataatttt acagaggtta ttgctgttat catacctaca
    13981 aacattccct tagaacccat ccaacctagt ggtatacctg ttaccatgta aactgattct
    14041 gttccttctg gtgtatatgg tattacaaat ggtgttatta taaaaaaggc aactattgca
    14101 actgctgctg atgctattgg gtctacatct atttgctttg cataagaata tccaatccct
    14161 aaaactgcaa gtagagtcat tacttcaaat gttgctacag ttggttgttg caattttaca
    14221 gcccaatctg gaccaaaaat ttgacctaca aattcattcc atccttttat aggaaaattt
    14281 gctactaata aaaataatga acctattatt aataatggtg atgatactaa aaaaccatct
    14341 ctaattgcaa ttaataattt atttttacct attttttcag ccaatggcat aagtattctt
    14401 tccaatctat tcatctatcc accctcattt cctattattt tttactataa ttctacgctt
    14461 gtttagattt tattagcttt atagcaagtt ttaatacttt ttccccattc atagttccat
    14521 aatcaacttc attgattata tctataagtt ttccttgagc ttcaaacttt ggttttaact
    14581 cctttagcat gtatttaact tgtggtccta ataatataca atctggattt ttttcttcaa
    14641 ctattttgtc aatttctgct atcggaaatg cctctacttc tattggtaaa ttgtgttcat
    14701 ctgctacctt ctgcatttta tctgcaagta aacttgttga cataccaaag ctacaaaata
    14761 aatatacttt tcttttcatt ttttaaatcc cccttaattt tttattttag tttttgaatt
    14821 tcttttctta acataatcat ttcttttata aggtttctct cactcataca agtcattaaa
    14881 tggtcttgag agtgaatcaa taaaatagat atatctgatt tatctccccc agcttccttt
    14941 tgtataagtt cagtttgtac tttatgtgct tttaaaattt cttcatttgc attttcaatt
    15001 aattcatctg cttttttaaa atcgttttcc tttgcatagt caagtgcctc ataaagcata
    15061 gattttgcat ttccagcatg aattataatc tttaaaacta tttcttccat tttttcctcc
    15121 taagttgctt ctctactttt agattattta taccttacaa acatataata tcttataaaa
    15181 acaaaaaaac atgcacaact ttttccatca catggaaaat tttgtgctta tccatagttc
    15241 actatgaatt ttatataaga atatctttta ccttttcaat gtcacattta cttagaatat
    15301 ctagcatatt ttcatcctct gttaaattag atatttcttt aaataattct ctaaatactt
    15361 ttctatcatc atctttttta tatgctaaca aaagtataat ctttatatcc tttccatttc
    15421 taatttttat tggtgttttt aaccttgcaa aacataaaac agattgtttt atatatttca
    15481 tgtctccatg agggaataat attccttttc caacattggt tggatatgac ttttctcttg
    15541 ttataataga ttgtaaatac tctttttctg cataatgttt ttcaattaat aaattagtta
    15601 aatatgttat agcttcataa atatcatcat gttctaactc tcttattaaa taatcagaca
    15661 tcattaatct gttcataagt ttatatgaag tgtctttttc ttttattttg tttgtgaact
    15721 ctttaagttt attctcatcc tcatcagtaa ataatggtgt tgttataaaa attggttttt
    15781 ggatattttc atactcttga aatgcaatta taaaatctat agtcttatta aagttatctc
    15841 tttttaaatc ttcctctctt aatgtttcaa taatttttat gttatcaaat ctcttttcta
    15901 tctttacttt taatagcata cttactccaa aactaaatgg acatacaata cttgcctttt
    15961 tatttgattc ccttttgctg tattttcttt ctagagaggt ttgaaagtga agtgtaagat
    16021 aaccaatttc atcttccgtt atttgttttc cttttaattc aatttctatt gaacttgata
    16081 ttactgtaaa taaaaaacaa aatttagttt ttatgttatc taaaagtgga ttttctaagt
    16141 aaacattgct tttcatttga tgcattgtaa cattcaaatg acatattaat tgttcataaa
    16201 gtaatatatc attattaaaa tttattcctg attcttctga tactaaattt ataatttttt
    16261 ttgtataact tgctaattct tcatttagat ataaagattt cttaatatta ttactattta
    16321 tttgtttgtt catacccaat attaaagatt ctataaacaa tttttcttca taatttaata
    16381 ctatggataa attagatttc aattcatctt ctatatccaa tataagcatt tttagcttaa
    16441 gctctttctt agtatttgat acttttaaag aatttcccag ccttattcgt attatattta
    16501 taagcaaaaa tagcatcaat tgcttgaatg atatatctgt aagtcttata tccatatcct
    16561 cctctatagt aagcattatg ttttttacaa ttattatatc tactatatgg aagtactcta
    16621 tttcatctat gtttaatctg tatttatcta tatatttaaa taagaatgat actaacatta
    16681 ttcttatgtc tttctcatcc ccttcaatat atataccact gcctttttta gtcgagatat
    16741 ttaaattata attatttaat gctactgata atttacttat ttcttctctg acaatatttt
    16801 tatttatgta caacttatta gataaatcat ttaaagtaac ttttttatta ttacaaagta
    16861 gtaaatttaa tatctcaact tgcttatgaa gctcactatt tactctttta acttcacctc
    16921 gcaacttctg atttaccagt attttgtctg ttccatctcc actaaattta atcccaatat
    16981 taggttttct tttgatataa gcattacaaa aagtagataa ccattcatca atatatttgc
    17041 aatcatttct tattgtcctt tctgaacact caaaatcatt ggcaatctta ttaacattta
    17101 taaactcttc ttttaatagc aatgttgtta ataaattttc ttgtctttta ttcaaacata
    17161 tcacctcttt atgactcaac ctaaatttat tatatataaa aaagagctat acataaaggt
    17221 atgctagctc tcttttaata tgatatttag ctgacctggt ttttgacccc acactcgcct
    17281 actggagggt tcgcttgggt tcgccgcact actactctct ctcgatttta ttatcggtag
    17341 gtcttacttc taagccgcac catgatcatt aacccaattt ttagtgttaa cttacgtgga
    17401 tccctttgcc tgcgactggc ttggactttc aaccacagac ctaaatatca cttattcatt
    17461 ttaatatttt aaactaaaat tgtcaatata taagattatt ccataatata attatcattt
    17521 gcttttgtaa gtagagtcaa tacaaaaacc ttattagcac ctgataactt taaaagttta
    17581 gaaatttcgt ttgttgttag accagtagta aatatatcat ctattaaaag tatattttta
    17641 ttatttatta atttaacatt ttctttaaca acaaatacat tttttaattc ttcttttcgt
    17701 tctttcttat tcaatttata tagcattctt gtatattttt ttctacttat gcaatctaat
    17761 aatggaatat ttactatttt acttaaatta aatgctattt tttgtgcctg attaaaacct
    17821 cttttattta gccttttttt atgaagtgga acaaataata tataatcaaa ttttatgttt
    17881 tctaaatata atttttcttt cattatttga gctatatact tagccataaa ggttttttgg
    17941 ttatacttca gccctaacac catctttttt gtcacatcat tgtattcaat acaagatatt
    18001 gatttatcaa aataaaagtc tttattaaaa caataactac actcttcaat aaattctttt
    18061 tcaattgaat gacgtattat tggttttcca cacttcatgc aaccatcttg tataaaattc
    18121 atttctttaa aacaactttt acatattgaa taagtatttg ttttctttat ggatttatca
    18181 caaattatgc aacttatatt ctcaggatat ataaaatcca aacatttatt aatcatattt
    18241 ttaaaattct taataaaatt atcattgcat attttcatat ttattctatc aacaaccctt
    18301 cttgtttaaa tttatttagc ttatatccta aatttgaata cctttgatta actctattat
    18361 tttttatcat atattcaaga tatttcacat ctccaactaa tacaactaat ttttttgctc
    18421 ttgttactgc tgtatataat aaattacggc taagtaacat aggaggagcc cacgctattg
    18481 gcaatactac aactggaaat tcacttccct ggcttttatg tattgttgta caaaaactat
    18541 ggtcaatttc atctaattca tcatagaggt atgatacaat tttagtctgg tcaaataata
    18601 tataaatagt tttcttatct ttatctatgt gataaacata tcctatatct ccattataga
    18661 taccttctcc actttctttt tggtcttctg tttcccattt ttttgtataa ttatttttta
    18721 cttgcattac cttatcgcca actctaaata atcttttaga aaaactttct tcaactttaa
    18781 atttttcttt tttgttaaga tacttctgta actctatatt gagattagta actcccaatt
    18841 caccttttct cattgaagat aaaacttgta tatcttttaa tttatcaact ttataaaatt
    18901 taggtaatct ttcattaacc aatcctatta tttcatttaa aatttcttca ttagttgatt
    18961 ttcttataaa aaagaaatct ttaccttttg tatttagata aagtggttct ccattgttta
    19021 tcttatgggc atttacaatt atcatacttt cttgcgcttg tctaaatatt tcatttaatt
    19081 taactacatt tataatatta gaattaatca tatccttaag tacatttcca gcacccacag
    19141 aaggtaattg gtcactatct ccaactaaaa ttactcttgt tcctaacttt atagctctaa
    19201 gtaaattgta cattaagata atgtctacca ttgatacctc atcaacaatt attacatctg
    19261 aatttattgg gtcttcttca tccttaaaaa aagttaaatc atcatctgtt gaaaatccca
    19321 tttctagcaa cctatgtata gtttttgctt ctttattaga agtttcactc attctctttg
    19381 ctgctcttcc cgttggagct gccaaagtca cacttttacc attattttca aatatatcta
    19441 tgatagcatt aatggtagtt gttttaccag ttcctggtcc accagttata attaatactc
    19501 cactattaac agattctttg actgctaaaa tttgtttttc tgctaactta attttcttat
    19561 cttcttctaa aacattaatc tcgttatcta tatctatttt taagtcttca aattcatact
    19621 gtgataattt tactatttga ctacatactc cattttcagc taaatagtat ggcataaggt
    19681 atataagatt ttcattgttt actttctcta tatgtatttt ttgattataa actagtatca
    19741 ttacacactc ttttattatt tctccattta gctccaataa tttttccgaa tcttgtatta
    19801 gaacatgctc tggtaaatat gtatgcccat tacttaaaga tttatttaaa gtgtataaga
    19861 ttccctgaca aattctatct ttagagtttt tatctatacc aattttactt gctatactat
    19921 cagctacttt aaatccaatt cctcttatat cttcagcaag ttggtatggg tttttattta
    19981 taacttctat tgcactgctt ttatattttt tatatatttt taggcagtaa tttggtgtta
    20041 ttccaaaagg agatagctgt attattatat ttctcaattc tctgtcttct tcataacttt
    20101 ttactatttg ttttactttt ttactcccta taccttctac ttcttgtagt ttttctggtg
    20161 aattttgtat cacctctaat gtatctacac caaacttatc aattattctt tttgccattt
    20221 tttcaccaat accatgtatc attccagaag ataagtaaac ataaattcct tctaaggatg
    20281 atggtgttac tggcataaaa ctattcactt caaattgagt cccataaatc ttatgattta
    20341 cccactttcc ttcaacttct atgctttctc caattgccaa agtaggcata catcctacaa
    20401 ccacaatttc atcattttca tttaccaggt gagctattgt ataaccatta tcttcgtttt
    20461 taaagactat ttcacttatc attccttcta atttttccat ttttatctcc taaaaatact
    20521 aataatcgct ccatttaaat gttagtataa ttttatatat taaaaccatg ataatcgtag
    20581 aaaagtttta tatttttata cttaacgtaa tttccaacta ttatacattt tatcagaaac
    20641 aaaaaaaatc tgcatccata aaatatggta ccatgaaata caggtaccat atttaaatag
    20701 ctattttatt ttaattactc tatatttaga gtataaatta aatttcataa gtatattaca
    20761 gtactaactt atttcagatt tattctcctt gtacccctca gttttagtaa ttcttctata
    20821 aagcatacat agtaaaatta aaattcccat gtaacctgta tatggatata gtgtacctac
    20881 taacttatca aatggcagta aacctcctat acaagctaat atacttacca ctattgttat
    20941 aattttaaac ttagggtggt catcttctac aaatcggttt gttactgacc ataataatgg
    21001 tacagctgta gtgtatattc ctagtaaaag tactactgag aataaaatac ctattattgg
    21061 agatatctta tcagctaagt aaagtgctgg tatctctttt gtgtatatat ttcctatgtc
    21121 tgaaagtaat gccaaattca tcattatagc tgctgccata agtgctactc cacctactat
    21181 accaccccat acagcatctt tcttatttac tgcagaagct cccattcctg tcaagaatgt
    21241 tattactatt attacattgt aacaagtata aagtactccc gagaaatatc catttgatgt
    21301 agcagtttta atatttaaac tatcaaccat atttccagca tttgataaag catcaatatt
    21361 gctaaataaa ctaattgctc ctactaaaag tgtaaatatt attattatag gtcctatatt
    21421 acctagtatt ttagaaagcc ttgtcaaacc taatgaaaca gttattaggg aaacgattgc
    21481 cataccaatc ctacctacat atggatttaa tccatagtac tctgataatg tagctcctgc
    21541 tcctgcaatc attattacta atgttccaaa taaaaacaat ggaccaaacc attcaaaaaa
    21601 tgttccaaga tattttccac aatatacttg ataaattttt attggctcct taagtttcaa
    21661 ttctcttccc ttatctataa cttctgctcc aaaccatgaa aatagtacca tagagattat
    21721 tccacctata atacccgcat atccataaaa cgtaaaaaat tgcagtattt cttgaccagt
    21781 tgcaaatcca gaacctatta cagtagcaac ataagctcca gcaaaactta taaccgtttt
    21841 aatatttact ttgcctgaca tacactactc ccccccaatt aaaattattt ttaaagtgta
    21901 agtttattaa gattatatta gactcattat tgtgatattt caatatattt ttaattattt
    21961 aacattagtt aacacattct taaccatcct ttaacatata tcccataata tgtataaatt
    22021 tagtagagta ttatataaaa tgtattgacc agattaaata ctgtttattc attatatgat
    22081 gataaattaa catttattaa aaatatttaa ctcataataa aaaagctgtt agaaagtaat
    22141 cgtaaaatta cattctaaca gctttttagt taaaaattat tcacctaaag catcttttct
    22201 taactgctca accttgtcag ttctttccca aggtaaatct atatcagttc taccaaagtg
    22261 accataagca gcaacctttt tataaagagg ctttcttaaa tctaagtctc ttattatcgc
    22321 tcccggtctt aaatcaaagt gtttatttac taattcaaca agtttttctt ctgaaacctt
    22381 gcctgtccca aaagtatcta taaatataga taatggtcta gctataccta tagcatatgc
    22441 aagttctatc tcacatttgt ctgcaagacc agccgcaaca atattttttg caacatatct
    22501 agctgcataa gcagcagatc tatcaacttt tgtagggtct tttcctgaga aagctcctcc
    22561 accatgtcta gaatatccac cataagtatc tattattatt tttcttcctg taagacctgt
    22621 atctccttgt ggtcctccta taacgaatct tcctgttgga tttatataaa ttttagtctc
    22681 ttcatcaagt aattctgctg gtataacttc cttaataaca tgattaatta aatcttctct
    22741 tattttatca tttgatactg tttcacaatg ttgagctgat ataagaactg tatgtactct
    22801 tacagcttta cttccttcat attcaacagt aacttgagtt tttccatctg gtcttaaata
    22861 atcaactaaa cctgtttttc taacttctgt aagtcttctt gataatttat gtgccaaaga
    22921 tatcggaagt ggcattaatt ctggagtctc attacatgca aatccaaaca ttataccttg
    22981 gtctccagct ccaacttttt ctatttcttc ttctatctct tctccagtct tactttctag
    23041 accttcatca actcccatag ctatatctcc agattgttca tcaatagaag ttataactgc
    23101 gcaagtatca caatcaaatc catattttgc tcttgtatat ccaatttctc ttactgtttc
    23161 tcttactaat ttaggaatgt ctacataagc agatgtactt atttctcctg ctactaaaac
    23221 taaaccagta gtaactgtag tttcacaagc tactctagat tgtgggtctt tttctaataa
    23281 tgcatctaat attgaatctg atatttggtc acatatctta tcaggatgtc cttctgtaac
    23341 tgattctgac gtaaataaat gtcttgccat ttttattccc ccttatttat tcttaatttt
    23401 tattataggt tttaccttat ttttaatttt agaaatttac tcaaaataaa aaaacctctc
    23461 ctttataaga agaggttaat aatcgtaatt acatactaat taactgacct catctttcaa
    23521 gacttttgtc ttgtaggaat tagcaccttt tcccatttgg gtggttgctg ggcttcacag
    23581 ggcctatccc tcagcctctc ttgataaggt aatcaagatt taagttttgt tttactttac
    23641 tagcttacta tttttttaaa attttgtcaa tatttttagt catatttttt ctattccttt
    23701 ttttgtaaga caaataaaat tttctatcta tctgaaattt aaagttaggt ctttttaatg
    23761 ctttttattc atatattttt ttgaaggggg tgttcctatt gtatttagct aaagtaaact
    23821 acagcgatga tgatgttata cagatgctaa gttcagttct aaaaactata attaatgaaa
    23881 atacgattgt tgtttgcata ggaacagaca gggctatagg tgatacttta ggccctttag
    23941 ttggaacaat acttaagaac agtaacttta aataccccgt ttacggtaca ctagataacc
    24001 ctattcacgc cttaaatata tatgagtctt tagatactat aaaaaataca catatacaag
    24061 gtaacttctt ggctatagat gcttgcttgg ggtcgcagag taacataggt aatatacaga
    24121 ttagagaagg acctatcctg cccggaaaag gtgttggtaa aaaactacct caaataggaa
    24181 attattctat agtaggaatt gtagataaaa ttgatgaaaa taataaactt tcatttaata
    24241 atatacgcct tagctttata ttagatttag ctgaaacaat agccttggct ttattagtgt
    24301 ccacctaatc taaatataaa ctaaatgata aaattcttta tataaaaagg tagatatttt
    24361 agagtatctg attaaatact ccaaattatc taccttttta cttttatatt tcaagttttt
    24421 atttttctac taaagcaaac attaattcac ctttacagac aagttcttca cctacatatg
    24481 ccttagcatc tgccttagct atattttttc taagagttgt catttcaact tccatagtta
    24541 atgtatctcc tggtcttact tctctcttga ttctaacttt atctatacct gcaaatacac
    24601 ctagttttcc tttgttttcc tctacagaca tcattgccac tccacatact tgtgctagag
    24661 cttctactat tagtactcct ggcatcaaag gatattctgg aaagtgacct tggaaaaatg
    24721 gctcattaac tgttacattc tttataccaa cagctctttt cccaacttct agctctgtaa
    24781 tcttgtctac taataaaaat ggatatctat gtggtattaa tttttttatt tcatctatat
    24841 ttaacattct aaactctcct aattattttc ttttgaaaga cccaccagtt tcaagtaagt
    24901 ctattgaacc taaagctatt cctgtacctt ttgcaacaca agataatggt tcatctgcta
    24961 ttgtaacagt tattcctgtc ctctgttcta ttattttatc taatccatat aataatgaac
    25021 ctcctccagt cattattata ccgacatctc ctatatctgc tgctaactca ggaggcgttt
    25081 tttccaaaac agcatgagta gcaactacta tttgctcaac acattctcta agtgcttcca
    25141 acatttctgt tgaatttata gttatagaac taggaagtcc tgtaattaaa tttcttcctg
    25201 ttatcttcat atatttttct tcttctctct taaatgcaga acctatttca aacttgattt
    25261 tctctgctgt tctttctcct ataagaagat tgtgttgttt tttcatatat ttaactatag
    25321 catcatcaaa tctatctcca ccaaccttta tagattcact tacaacagct cctcctagag
    25381 atattacagc tatatctaca gtacctccac caatatctat aatcatatta ccattaggct
    25441 gtgatatgtc tacaccagct cctatggcag ctgctattgg ttcttctatg atatatactt
    25501 ctctagctcc agcttgtctc gtagcatctt ctactgctct tttttccact tctgtgacac
    25561 ctgttggtac acataccata attcttggca taaaaaatct tccaaatccc tttttatcaa
    25621 ctatcttatc tataaaagat tttaacatct tctcagttac atcataatct gaaattacac
    25681 catctcttaa aggtcttata gcgactatat tacctggtgt tctccctatc atctttttag
    25741 cttcttctcc cacagctaaa acagaatctg ttcttttgtc tatcgccaca acagatggtt
    25801 cttctaaaac gattccctta ccactaacat aaacaagcac atttgcagta cctaaatcta
    25861 tgcctatatc agctccagcc attttttcaa ctcctttatt tataatatta gattatatta
    25921 taagcctatt ttaatactcc tataataaag catatacaaa aatctcgctt taatcaagag
    25981 aaaataaatc ttttgagagt at
//
LOCUS       DAEORZ010000045        25603 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEORZ010000045 DAEORZ010000000
VERSION     DAEORZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.045"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            109..300
                     /locus_tag="KRH39_001698"
     CDS             109..300
                     /locus_tag="KRH39_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBG8511611.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            455..1864
                     /locus_tag="KRH39_001699"
     CDS             455..1864
                     /locus_tag="KRH39_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG8511612.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            1995..2684
                     /locus_tag="KRH39_001700"
     CDS             1995..2684
                     /locus_tag="KRH39_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBG8511613.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCILDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2687..3622
                     /locus_tag="KRH39_001701"
     CDS             2687..3622
                     /locus_tag="KRH39_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBG8511614.1"
                     /translation="MYFENIIGQNFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3619..4512
                     /locus_tag="KRH39_001702"
     CDS             3619..4512
                     /locus_tag="KRH39_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBG8511615.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4590..5336
                     /locus_tag="KRH39_001703"
     CDS             4590..5336
                     /locus_tag="KRH39_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBG8511616.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLELKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5337..6170
                     /gene="rsmI"
                     /locus_tag="KRH39_001704"
     CDS             5337..6170
                     /gene="rsmI"
                     /locus_tag="KRH39_001704"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBG8511617.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6193..6897
                     /locus_tag="KRH39_001705"
     CDS             6193..6897
                     /locus_tag="KRH39_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBG8511618.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGINLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYTDIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7085..7513)
                     /locus_tag="KRH39_001706"
     CDS             complement(7085..7513)
                     /locus_tag="KRH39_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG8511619.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7550..8980)
                     /locus_tag="KRH39_001707"
     CDS             complement(7550..8980)
                     /locus_tag="KRH39_001707"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBG8511620.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9112..9696
                     /locus_tag="KRH39_001708"
     CDS             9112..9696
                     /locus_tag="KRH39_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBG8511621.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9712..10233
                     /locus_tag="KRH39_001709"
     CDS             9712..10233
                     /locus_tag="KRH39_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBG8511622.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANIMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10299..10545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10629..12566
                     /gene="metG"
                     /locus_tag="KRH39_001710"
     CDS             10629..12566
                     /gene="metG"
                     /locus_tag="KRH39_001710"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBG8511623.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12596..13366
                     /locus_tag="KRH39_001711"
     CDS             12596..13366
                     /locus_tag="KRH39_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBG8511624.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13704..14135
                     /gene="phnG"
                     /locus_tag="KRH39_001712"
     CDS             13704..14135
                     /gene="phnG"
                     /locus_tag="KRH39_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBG8511625.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14150..14725
                     /gene="phnH"
                     /locus_tag="KRH39_001713"
     CDS             14150..14725
                     /gene="phnH"
                     /locus_tag="KRH39_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBG8511626.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14728..15813
                     /locus_tag="KRH39_001714"
     CDS             14728..15813
                     /locus_tag="KRH39_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBG8511627.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15833..16702
                     /locus_tag="KRH39_001715"
     CDS             15833..16702
                     /locus_tag="KRH39_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBG8511628.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16695..17561
                     /locus_tag="KRH39_001716"
     CDS             16695..17561
                     /locus_tag="KRH39_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8511629.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17602..18315
                     /locus_tag="KRH39_001717"
     CDS             17602..18315
                     /locus_tag="KRH39_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8511630.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18316..19485
                     /locus_tag="KRH39_001718"
     CDS             18316..19485
                     /locus_tag="KRH39_001718"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBG8511631.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19489..20325
                     /locus_tag="KRH39_001719"
     CDS             19489..20325
                     /locus_tag="KRH39_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG8511632.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20326..21321
                     /locus_tag="KRH39_001720"
     CDS             20326..21321
                     /locus_tag="KRH39_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8511633.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYSSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21339..23027
                     /locus_tag="KRH39_001721"
     CDS             21339..23027
                     /locus_tag="KRH39_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG8511634.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAVFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23039..24106
                     /locus_tag="KRH39_001722"
     CDS             23039..24106
                     /locus_tag="KRH39_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG8511635.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     LKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24543..25538
                     /locus_tag="KRH39_001723"
     CDS             24543..25538
                     /locus_tag="KRH39_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8511636.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHDGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSDIGKKVRDILEVVELSGYDKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIKSLHRKLGITTIMVTHDQEEALCLADKMVVMNNGEVIQIGTP
                     KEVYKSPKTPFVADFIGTINFIDDDVNMIAIRPEDIKVEFNRDFKEIDVKVGEILDIE
                     FRGFNYRITVEYCSKQIKLDVVSKVVEQMKLCVGSKINFKIPKERTIQYKSEGCA"
BASE COUNT        10235 a         2841 c         4589 g         7938 t
ORIGIN      
        1 ttttttgtca aaaaaaacaa aaaatacgta tttctttttt catttcggga aaactaaaca
       61 caggaattat ggactttata aaaatttaat tcttgaggag tgatataagt gaaaaaagtt
      121 gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat tctaggtaca
      181 gacttaatag gtagtatctt tggtggaaca tatgaaatgg taagtagaat aatatacttc
      241 attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa
      301 tttatgcttt tatgttaaat acctcattct aaatgtttag aatgaggtat ttaattttat
      361 aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa gttttaaaaa
      421 gaagtattat gtaaaaaagg agtactatta atatatgttt attattaatg aattaaataa
      481 gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaaagg gacaggttta
      541 taaaaagtta ggatataaag atatactaaa aaatttgtat aaaatggata caactgagat
      601 attaggaaca gataatttac actttccgga aggaattata aaaaattcac aaaaaaaagc
      661 atcaagagta tttaaaagtg attatacttg ctatttagta aatggtagta gctgtggaat
      721 acaatctgca ataatgtcag tatgtagtcc taaaagtaaa ataataacaa atagagactg
      781 tcatcagtct gttataaatg gatgtatttt aggagatata gatatagagt atgtgccttg
      841 tgagataagt gaagagacaa atgtcttaaa agggatatct ataagtaatg tgattgatat
      901 aatagataaa aacttggatg caaaagcagt actgcttact tatcctacat attatggaat
      961 gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaagatgg tagtgatagt
     1021 agatgaagca catggtgcgc atttagggct tagtgagagg cttcctaaaa cagctcttga
     1081 gcaaggcgca gatatagttg tacagagtac acataaaact cttccagcat ttacacaatc
     1141 gtcaatgata catatacagg gagaccgagt tgatatagat aaaatattga acatgttaag
     1201 aattacagaa tcttcaagcc cttcatattt attattatct tctctggaat tagcagtaga
     1261 tatatatgaa aataaaggaa aagaattgat ggaagagctt ttaaataata ttataacttt
     1321 caaaaataaa gttgattcaa atattaatat atacgacaca aatgataaaa caaaaatatt
     1381 tatatcatct aaaaacatgg gtttaacagg ttatgaacta gaaaatattt tgagaaataa
     1441 atataacata caagtggagt tatctaatta ttatggagtt cttttaatat gtacaatagg
     1501 aaatactgct gaggatttct ctagtttaga aacagcatta aacaatgttg taaaggaaga
     1561 ctttaaattt gaaaagttag aaaataaaaa ataccctgtt gttattccaa agaaaatatt
     1621 aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg acagcatagg
     1681 taaagtttgt ggtgaatgta tagttccata cccacctgga ataagtgtaa tatctcctgg
     1741 tgaagtaata agtaaagaaa taatagacta tctaatgttt tgtcattcta aaggcatgat
     1801 tgtgagtgga ttgaaagatg tagatttaaa ttttatacaa gtaatagatt tagaagacat
     1861 ttaaaaatta tgatataaaa gtagatagaa gtagatagtt taaaattgtg aagagaaata
     1921 aatagactgc ttatatagag ctatctaata aaagtactaa aaaagtaaaa agtgtttgta
     1981 tggaggatat gaaaatgaaa ggtaagttaa taattataga gagtggttca gatgcaagtg
     2041 gaaaggctac tcaaacagaa atattatatg aaagattaaa aaaagaaggc tatatgatta
     2101 aaaaggtaga atatccaaat tatgaatctc catcatctgc cttggtgaaa atgtacttaa
     2161 aaggagactt tggtaaaaat gcatcagatg tagacccata tatagcatca acttttttta
     2221 cagctgatag gtatgcatca tttaaaaccg attgggagga gttttacaat gaaggtggaa
     2281 taattatcgc agatagatat acaacctcta atatggtaca tcaggcctct aaaatggata
     2341 tttgtgatag agaagagtat ttaaattggt tagctgatta tgaatttaat ttatttaaga
     2401 taccacaacc tgattgtgtt atatttttag atgtgcctat tgaatttagt aaaaagctta
     2461 tggagaatag aaaaaataaa attacagggg aagaaaaaaa ggatattcat gaaagtgaca
     2521 tagagtattt agagaaatct tacaacaatg ctttatatat agcagataaa tatgattgga
     2581 aaaaaataaa ttgtatttta gataacaaat taagaagtat tgaaagtata cataatgaaa
     2641 tttataaaat agttcttgat tcaataaaac ctatggagag ataaatatgt attttgaaaa
     2701 tattataggg caaaattttg caaagaaata tttaacaaat tctataaaga aaaataaact
     2761 taataatgcg tatatgtttg aaggaatgga tggaatagga aaaaagaaat ttgcagatga
     2821 attatcaaaa ctattacttg attatgaaaa tctcgaaaat agtccagact atgtactcat
     2881 aaaaccagat ggaaatagta taaaaatagc tcaaatcaga aatttacaat cagatatagt
     2941 aataagacct cataaagact ataaaatata tataatcaat aatgctgaaa aaatgactgt
     3001 ggaagctcaa aatgcacttt taaagacgct agaggaacct ccaaactatg ctataataat
     3061 cttagtcaca aacaacaagg aatctttatt agaaactata aaatctagat gtgatataat
     3121 aaagttctca cctataccaa ttgaagactt aaaacgttat ttgataaata caggtataga
     3181 agaagaaaga gctcaattat tggctatttt ttcgagagga agtattgaga atgctctaaa
     3241 tctatctcag tcttctgagt tttcaatgat gagagaagat atacaacagt atattcagat
     3301 aatgttagat aaaaacatag tggagatttt aaatatacca aataatatgg aaaaatatag
     3361 aggtaagatt atagctttat tagatatgat gataaactat tttcgagata tcatcctatt
     3421 aaaagaaaat gtaaacaaga acatgttaat aaatgtagat aagctagttt tcattcaaaa
     3481 tatgagtgga aaaattagtt attctcaatt gtccaagatt attgatataa tagaagatgc
     3541 aaagagtaaa ataaaaagca attgcaattt caatataagc atacaagtta tgtctttaaa
     3601 tatatacgag gtgattaaat gataaagata gtaggtataa ggtttaaaag tgctgggaaa
     3661 atatattatt ttgaccctgt tgattttaat atagagcaag atatggatgt agttgtagaa
     3721 actgcaagag gcttggagta tgggaaagta gtcgttggtc caaaagatat ggatgaaagc
     3781 gaattagttt ctcctttgaa gccaattata agaatagcta cagaagaaga taaaaaagta
     3841 tatctagaaa ataaggaaaa ggcaaaagaa acttttgagt tgtgccaaca aaaaataaaa
     3901 gaacatgagt taacaatgtt tttaatagat tgtgagtata cgtttgatag gaataagttg
     3961 attttttact ttacagcaga aggaagaata gattttagag aattggtaaa agacttagct
     4021 gctatattta aaactagaat agaacttaga caaataggtg tcagagatga agctaaatct
     4081 ataggaggtc tagggccttg tggtagaaaa ctttgctgtt catcttggtt aggtgatttt
     4141 caaccagttt caataaaaat ggcaaaagac caaagtctat ctctaaatcc aacaaaaata
     4201 tctgggatat gtgggagatt attctgttgc ttaaaatatg agcatgatgt atatagtgaa
     4261 gcaatagaaa aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg aaaaggaaaa
     4321 gttgtagaag tgaatccttt attagaacaa attaaagtgg aatttcaaga caagacagta
     4381 aagatataca caagagaaga tataaaaata cttagagagc ctaaaaaatg tgatggctgt
     4441 ggtggtaaat gtaaggataa tgatggatta gatgaagcca cactaagaga gctaaagaaa
     4501 ttagaagatt aaatttaagg tagggctttt cctacctttt ttaaagcaat taatgcttaa
     4561 agttattaat atttgaggag aatttatata tgcaattaaa agattcagaa agaatagatg
     4621 atttacaatt aaaaggcctt aaaattatac aagatactaa tggtttttgt tttggtatag
     4681 atgcagtttt attagccaat tttactaaaa taaaaaaaga tgccaaagta gtagatttgg
     4741 gtactggaac tggaattata cctattttga tagctggaaa aagtgaagct aaaaaaatta
     4801 ttggcgtgga aatacaagaa gatgtatatg aaatggcaac tcgttctata aaattaaatg
     4861 gattagaaga aagagttgag attataaatg aagatataaa atacatagac aaagtcttag
     4921 acataaatgg ttaccatgta gtcacatcaa atcctccata tatgcatatt gatggaataa
     4981 aaaatccaaa tgataaaaaa gcaatatcaa gacatgaagt aaggtgcaat ttggaagatg
     5041 taattagagc tgcatccaga ttggttatgc ctagaggaaa attcttcatg atacatagac
     5101 caacaagatt ggttgatata ataactttag ggagaaaata taaattagaa ctaaaggtta
     5161 ttcaatttgt gcatccaaga cctcaaaaag caccgaactt agttttggtt caatttgtaa
     5221 aagatggaag gccagagctt aaaatattgg acccattgta tgtttatgga gaagatggga
     5281 attatacaaa agaattaaaa gcaatatata ataataaaga tataggagag ttgtaaatga
     5341 gcgggaaatt gtacatatgt ccaacaccaa taggaaacct agaagatata acatatagaa
     5401 ctttaagaac attaaaagaa gtagacttga ttgcagcaga agatacaaga catagtataa
     5461 aacttttaaa tcattttgaa atctcaaaac cactaactag ttatcatgaa cataataagg
     5521 attcaaaggg agattatttg ataaataaac taattgatgg tgaaaatata gctctgataa
     5581 gtgatgcagg tatgcctggt atatctgacc caggagaaga gataataaaa caagctatac
     5641 aaaataacat agagatagaa gtattgccag gtgcaacagc ctttgtaacg gctttagttg
     5701 gctctggtat ggacacacat agatttgttt ttgagggctt tttagataga gataaaaaag
     5761 ttaggaaagc acaattggaa gaattaaagg aagaaagtag aactataatt ttctatgaat
     5821 caccacatag attgaaggat acactaaaag atatgcttaa aattcttggt aataggaata
     5881 tttctataaa tagagaactt acaaaaaaat atcaagaaat aataagagaa aatatagaaa
     5941 ctaccataaa aatatttgag gaaaaagaag taagaggaga gtttgtatta atagtagagg
     6001 gttttcatgg tgagaagtca aagaaagaaa attatgatgg gcttacagat agggaatatg
     6061 ttttaaaact aattgaaagt ggtatgaaca aaaaagatgc aataaaaata gtttgtaaag
     6121 atagaaagtt aaagaaagat gtggtttata aacaagtttt agatttataa aacctgaatc
     6181 tgaggtgatt ttatggaaat agggaaaatt ataatagaga gtttaaagag ggaagataca
     6241 cataaaatat ttatagaatt agataataag catgaattag aaaaaataat tcctaaaata
     6301 agctgtatga agtctgtagg tgaatgtaaa tatcatgtag taaattgttt tgaacattct
     6361 ataaatgcat taaaagaatt agaaatagta ttaaatgata aggatttttt tccaatacat
     6421 ctaagaaaac atgtgtcaaa ctatttaaac acatatattg aagaaggaat aaataagtta
     6481 catgtattaa agctgggaac atttttacat gatataggta agcctgacag tatgacctta
     6541 gatgaaaccg gaagagttca tttcacaaat catgaaaaaa taggtgctca aataattgac
     6601 aatatgggaa taaatttaga gttatcaaca gagacatata gacttataag taaatatgta
     6661 agatatcata tgatactttt gtctctatat aagaaaaatg atttgagtag gaaagaatta
     6721 attaatgtat ttaacttagt agatgaggac actataggtg taataatgct tgggtataca
     6781 gatatagtgt ccacagtaaa attattgggt aagagtggag aagtcagtgt gttaaagaca
     6841 tatatggaat atattttaac aaattacttg tataagagta attatacaca tgtataaatt
     6901 tagttaatat aattgagttt tagatgatta attaaagttt gaaatatacg gatttcaaaa
     6961 acataaaaga agttgtcttt gttgtaaaat tatgaaagac aacttttttt atatcaaaaa
     7021 ttgaaatttt attaaggata cttaatggtc tattaattaa taaagcatta gtatttaatt
     7081 tatattacaa tattgatttt gcttgtattg gcgttgatag tataacagag gtcttagtag
     7141 aaccaatttt ctttatagta tcaatcacat tttctagctc gtacatatct tttacaatta
     7201 cttttaaaag taagcaatca tcacctgtta tatggtgaca ttctacaatc ctagggtcct
     7261 ttgcagctga ctcaataaat tctgtatatc cattgcttgg aagagaaata tgaataaatg
     7321 cctttataac tctgcctaat gaatctgggt tgacaatagc tttatatcct tctataacac
     7381 cagactcttc taatcttttg actctttctg aaactgcagg agaagttaaa ccaactattt
     7441 ttcctaagtc cttcatagaa attctcccat catcctgtaa aatttctatg attctgtaat
     7501 ctgtaacatc cataaataaa cagtccccct taaataaaaa taattaatgc tataaaactt
     7561 ttttagaatc tatcatttgt gtttttaaat accaaagatt tctagataaa tcaaccttat
     7621 aaatgtgtgg ttttgaaagt cttttgtatt gttcccagat agtacttaat tcatattgta
     7681 catgattttt tatttcaaga acagcttttt tattatattt gcatctgcct tttatataca
     7741 atggtttaag aagctcttta actttgtaat ttgtaaatac cttcgttttc catgtataag
     7801 taggatggaa gatttctaaa ggtttagact catctataac ttcatcatgt aacataatca
     7861 aatctgcctc tgccttattg tcttcgttat atattcttat aactttcttg taaccaggat
     7921 tatttatttt ttctggattc tcagaaatct ttatcttagg ttcaagaact cctttttcac
     7981 aagatgcagc taatttataa actcctccaa gagaaggtga atcgaaagat gtaatgagct
     8041 ttgtacctac tccccatgaa tttatagtag ctccttctgc tttaagtgat gttatggtat
     8101 attcgtctag gtcattagat gcagtgatgc tgatatctga aaagccagca ttatctaatt
     8161 gttttttagc ttctttagat aaataagcta aatctccaga gtcaagtctt atacccatag
     8221 gtttgtatcc tttttcagaa atatttttga atacttttat tgcattagga actccactgt
     8281 ttaatacatc ataagtatct acaagtagta ggcatttatc tggataaaca tcagcatatg
     8341 cttggaatgc ttcaagttca ttatcaaatt tttgtaccca actgtgagct tgtgtaccaa
     8401 ttataggtat atcaaacatt ttccctgcta gtacgtttgc agttccagca caaccaccaa
     8461 ctacggcagc cctagcccca tatatccctg catctggacc ttgtgcacga cgtaatccaa
     8521 attcaaatac agggtctcct tgagctgcaa agcaaactct agatgcttta gtagcaataa
     8581 gagactgaaa attaactatt gttaacaatg ctgtttcaat aagttgagct tgatataaag
     8641 gtgccttaac agtaattaat ggctcattag ggaacatgat agttccttcc tcaacagcat
     8701 atatatctcc tgtgaattta aattctttta aaaattttaa gaacttgtca gaaaataaat
     8761 ttaagttttt taagtactct aaatcttcat ctgaaaaatg aagattatct atgtattcaa
     8821 caacctgatc aataccacaa actatagtat accctccatc acaagcgttt tttctaaaaa
     8881 acatatcaaa tacaacaatg tcttcatgaa tattcttttc aaaatagcca tttaacatag
     8941 taagctgata taaatcagta agaagtgtta gatttctcat tatttctctc cttaattatt
     9001 attaaatatt aaaaattgta aaaactccca cacttatact aaaacataat atagtacaac
     9061 tttcaagtat aaagtattac ctaactatca taaaattatt taaagaggag gatgattata
     9121 ataggaaaaa tatggtttta tatggtatcg ataggaataa taggaagctt gatttctaaa
     9181 aatatgggtg aattaaataa agttatatta agtgaaacat caaaaggtat agagtttgca
     9241 ataagtctag ctggtatcat ggcactctgg atggggataa tgaatatcgc aaaagaatca
     9301 ggtttaattg aaaagatagg tcaaaaacta aatcctataa tgagaagatt atttccttct
     9361 attccaaaag gtcataaagc aatgtcttat atagttatga atattgcttt aaatatgttg
     9421 ggtgctggaa atggtgcaac agcatttggt ttaaaagcaa tgagtgagct tcaaactttg
     9481 aataacaaga aagatacagc cactaatgat atgattatgt ttatggtaat caatatatca
     9541 tctatacaaa taattccatt tactataatc aaattaagga tggatatggg ggctcaaaat
     9601 ccaagtgaaa taatttttac gactttattt gcaaccataa tatctacagc agtagcaata
     9661 ataacatgta aattttttca aagaagatat agataaaggg gatgtagata catggagata
     9721 ttttcaaact tacttatacc aataataatt ctgtacatag tagtttatgg taaatataaa
     9781 aaaatagatg tgtatgatag ttttgtaaaa ggagcaattg atggattaaa agcagcatgg
     9841 gatatcctac catatataat aggtatattt ttggcaatag gaatattcaa aactggtaaa
     9901 ggtcttgata tgttagaatg gctatttaca ccaatagcaa atattatgag tataccaaaa
     9961 gaattaatag gtctaataag tgtaaaacct ttatctggaa gtggtgcttt agggatgtac
    10021 agtgaacttg caaatagagt tggaatagga agtttagtag aaaaaatggg tgctacgatt
    10081 gtaggttctt ctgagactat tttttacaca atggcaatat actatggaag tctgaagata
    10141 aaaaatacta gacatacatt atcatgtgct atgattagcc atgttgcagg tgtcatagca
    10201 gctgttttta tttgctatgt aatatttgta taatttgttg acaatatggg aaaaatttta
    10261 taatatagtg attatataga aacaaatagt aatacaaatt acaagctatg aaaagaagag
    10321 taaataaagg ttttttctat cagagagcta ggttaggtga aagctagtgt caaaagcttt
    10381 attgaagatg gtcttggagc tttttatctg agcaagtgtg ctagggtaaa acggttggac
    10441 acgttatagt ctactaaggt atctataaaa tgtttctaca tttcatgtga aacataacta
    10501 ttatagatga attagggtgg taccgtgaat aatctcgccc ctatgttaac tcataggagg
    10561 cgatttttta ttatttaaat gaaagaatca tatgattacg aacgataaaa aattaggagg
    10621 taaataacat gagcaaaccg agtttttatg taacaacacc aatatactat ccaagtggag
    10681 gattacacat aggacatact tattcaacag tagctgcaga tacaatagct agattcaagc
    10741 gtttctgtgg atatgatgta aagttcttaa caggaacaga tgagcatggt gaaaaaattc
    10801 aaaagaaagc catagaacaa ggaatgtcag agatagagta tcttgatgga atgataaaag
    10861 atataaaggc cttatggaat actatggata tatcttatga tgattttata agaactacag
    10921 aaaaaagaca tacagacata atacaaaaga tatttactaa actatatgag caaggtgata
    10981 tatataaagg tgaatatgaa ggtagatact gtactccttg tgagagtttc tggacagaat
    11041 ctcaattact agaaggaaat aaatgccctg actgtggaag agaaacatat ttagtaaaag
    11101 aagaatctta tttctttaga ttatctaaat atgaagacag attaaaagag ttatttaaag
    11161 atgatagttt ctgttttcct gctgctagaa aaaatgaaat ggtggcaaac tttttagaca
    11221 aaggattaga agatttaagc gtaactagaa cgacttttga ctggggtata aaagttcctt
    11281 ttgatgaaaa acatgtaatt tatgtgtggg tagatgcttt atgtaactat ataacagcat
    11341 taggttatat gactgataat gatgaagaat ttaaaaaata ttggcctgcc aatgttcaaa
    11401 tagtaggtaa agaaatagtg agattccata caataatatg gccagcgctt ttaatggctt
    11461 taggtctaga agttccaaaa caagtatttg gacatggatg gatactattt gctgatgata
    11521 aaatgagtaa atcaaagggt aatgtggttt atccagaacc tataatagaa agatatggaa
    11581 tagacacact taagtacttc ttattaagag agtttgcatt tgggcaagat ggaagttata
    11641 ctcatagaaa ttttgtaacg agaataaatt atgatttagc aaatgattta ggaaacttaa
    11701 taagtagaac tgtagctatg gttgaaaaat acaataatgg tataatacca acagcaaaag
    11761 tttcaactaa ttttgatgct gacttaaaag aacaagctgt ttcaactaga gaaaactttg
    11821 aagctgaaat ggataaaatg caattccatg aagcattaga gagtgtatgg aaattagtta
    11881 gaagaactaa caaatatata gatgaaacta tgccttgggc actagctaaa gatgaaacga
    11941 agaaagatga attagacaca gttttatata atttatgtga atctataaga ataatcgcaa
    12001 cactaataaa tcctataatg aatgagactg ctaataaaat atatgaacat ataggaataa
    12061 aaggtcaaga tgatataact acatgggaaa gtacaaaaac atttggtctt attggagaaa
    12121 atgtaaaagt atttaaaggt gaacctttat tcccaagatt agatgtagaa aaagagatag
    12181 aagaattaac taaaatgttc tctgggaaac ctccagtaga agaaaaacct ttagagcata
    12241 aagaagaaat tactattgat gatttagaca aaatagagct tagagtaggg aaaataataa
    12301 gctgtgaaaa acatccaaaa gcaaataaat tattagtatc acaagttaag attggaccag
    12361 aaacaagaca aatagtatca ggaatagctg aatactataa gccagaggat ttagtaggaa
    12421 aagaggttac agtggtatgt aacttaaaac cagttaaatt aagaggtgta gaatctcaag
    12481 gtatgatatt agcagcaggt gatgatggag acccatatgt tttaccattt acacaagggg
    12541 ctaaagatgg atgcgaggtt cgttagtagg taattaaata gtaaggagat taaagatgtt
    12601 atttgactca catgcacatt taaatgatga aagttttgat gaagatagag atgaacttat
    12661 tggttcatta aaagataaag gtgtagattt ggttgtaaat ccaggagcag atatagagac
    12721 gtcgataaca gcaattgaac ttgcgaaaaa atacgacttt atatattcag cagttggggt
    12781 acatccacat gatgtatcaa aactagatga tacagcaata gaaactctaa gaaaacttgc
    12841 aactgagaat gaaaaagttg tagctattgg agagattgga ttagattatt attatgatta
    12901 ttctccaaga gaagaacaaa aagagtggtt taagaagcaa attgaattag ctaatgagct
    12961 aaaacttcca ataataatac atgatagaga tgcacatgga gatacttttg agataataaa
    13021 gaatactaaa aatcctgaaa taggatgtgt acttcattgt tatagtggga atgtagagtt
    13081 agctagagaa tatgttaaga tgggttgtta tatatcaatt ccaggcacgg ttacttttaa
    13141 gaataataaa aaaactagag aagtagtaag agaaatacct ctagaaagat tattcataga
    13201 gactgattca ccatatatgt caccagagcc acatagagga aagagaaaca atccttccca
    13261 agtatctttt gttgctgata agatagccca ggaaaaaggg atatcttatg aagaagtatg
    13321 tagagttaca aaagaaaatg caaagaaatt ttttaatata aaatagacaa attaaatctt
    13381 ataatgatat atataataag tcattataag tataggatga gagtgcctca aaatgagttt
    13441 tttagttcat gaggcactct tttttttatg aaatcaaata tattttcatc atttcaacaa
    13501 tgaaaaagaa gtttatcata ttttgagata gtttgttttt atttatttaa ttcatatttt
    13561 aaaatatatt ttcttaacat tgttaaaaaa gaatttacaa gatattcatt agaattaact
    13621 caactctaat attcacttaa cttaagttgt ttattatatt attaaagaga agtaaacgaa
    13681 aggtagatta aggaggttga attttgtata cagttgatag tactaagata ttgataaata
    13741 tgaacttaga gaaactagaa aagatatact caatgataaa agatacatca agtatttcta
    13801 taattaaaga acctaacttg gctacagtaa tggtaagagc taatgaaagt gtaaaaaaca
    13861 caacatttaa tttaggagag atattagtta ctgaatgtag tgtaaaggta gatgaaagct
    13921 tgggttacgg aatagtttca gaaaacaata ataagaaagc catttattta gcagttatag
    13981 atgcagtttt acatagcaat aacagtaaat ttgatgagtt aaagaattac ataaataaaa
    14041 gtgtatatga agagaactta aggtatgaac aagagattat tgatgaattt agcttgatta
    14101 ataaaacgaa agttcaattc aatgctatgg attaaatagg aggagaatca tggtaaatta
    14161 tacacaagaa atatatagaa agatgctaga tagtgcttca tttcctggga aattaaataa
    14221 tatagataat ataaatatag aaaataatac aaaactattg aatggagcaa ttggtatagc
    14281 aatcacttta ttagaccaag aagttacctt ctatatagaa aattatactc aaaaagatat
    14341 aaaaaatata agagcactta caatgtcaaa tcaaatagat tatagaaaat cagattatat
    14401 atttttagat attaattcag aagtggatat attacagatt aaaataggtt cttttgaata
    14461 tcctgatgaa agtgcaacta tagtacatca agtaaatgat ttatcaacgt actctcaaac
    14521 taagtatata aagcttgatt tgagtggtcc tggtataaaa tctaaaaata gtatatatat
    14581 aaatggaatt agagaagaat ttatagaaag attatctagc ataaataaag attatccaat
    14641 aggaatagat ttgattttag tagataaaaa aggagaaatt gcatttatac caaggtcatc
    14701 aaaattaagt tgggaggtat tatagaaatg gcttatgtag cagttaaagg tggagaagaa
    14761 gccatattac aggctaaaaa tatacttgag ttttataggg taaaaggaga tagcttgcct
    14821 atagatgtaa agcagattga ggaacaaatg tcttatgctg tagacaaagt aatgtctgaa
    14881 ggaagtttat acaacaagga actatcagca ttggctataa aacaagctca aggtgatata
    14941 ttagaagcct ctttctatgt aagagcttta aggtcaacaa tgcctagaat tacatattca
    15001 gaacccataa atacagaaga aatgagaata aaaagaagaa tatcagctac ttttaaagat
    15061 ataccaggag gtcagtatct tggggcaact agagactatg aaaatagaat attgaatgta
    15121 gaattattag aagaagatga aaaaaagaat agacatttta gagatagttt tataaatgaa
    15181 ttagaaacag tagatgaaat ccctgaattt acaaaaatat ctaatatatt tagaaaagaa
    15241 aatttattac aagaagaagt ttgtgaaaac aaattggaag aagttattga tgatattaca
    15301 agaaatagtt taagatttcc agtttctagg tcagcaaagt tacaggcatt aactagaggt
    15361 gaaagtggag tagtgtcagg ttttgcttat gctattgtaa gaggttttgg tgatgaacat
    15421 ccatatataa gtgaacttag aactggtaat gtatttataa aagtaaaaca tcccatctat
    15481 gaagatgaag aaataataat aggggatatg ctggttactg aatgtgaaat agtttcaaaa
    15541 gcatcacaaa ataagactga aaattctaaa actaattcgg aagataaaaa taagatgtct
    15601 ttaggatatg gtctttgtat ggggaataat gagaataagg ctatatctat ggccatacta
    15661 gacaaatctt tggagccaaa tagtgaaaaa ctagcacaag atgaagaatt tgtattgctt
    15721 catatagatg gaatagattc attaggattt atatctcact ttaagcttcc tcactatgta
    15781 gattttaaag cggatgtaga aagaataaaa tagtttgagg aggtaagttt aggtgatata
    15841 taactatggt tttttagacg aaaacacaaa aaaagaaata agaagaaaga tattaaaagc
    15901 aatatcaata cctggttatc aagttccctt tggttcaaga gagttgccta tagctaaggg
    15961 atggggtact ggaggtttac aattaacact atcattaatt ggtaaaaatg atgtagtaaa
    16021 agtaattgac caaggaagtg atgattctac aaatgcttgt aatataagga gctttattgc
    16081 aagtgtaagt aatgtagaaa ccacaaaaga tacattagaa gctacattga ttcaaacgag
    16141 acatagaata ccagaagaaa aacttaaatc taatcaaata ctaatatttc aagtaccaat
    16201 accagaaacc cttagaatag tagaacctag tgaagtagaa acaagaagaa tgcattcaga
    16261 agaagattat agtaggatgt gggtatatct ttatgaagat atagttagat ttaatgatat
    16321 atctatagca gttgaatatc catgtaaagt aaatgataga tatttaatga atccatcacc
    16381 tatacctaga tttgatataa aaaaattaaa tatgtcagac aatttattct tgtttggtgc
    16441 aggtagagaa aaaagaattt atgctattcc tccatataca aaggtagaac ctttagagtt
    16501 tgaggattat aagtttgaag aggaaaagtt tgaaggaaaa tactgtagtt tatgtaacag
    16561 tacaaataca tttttagatg aagtatatga cagtgacact aatgaaaagt actatagttg
    16621 ctcagataca agttattgtg aaaaagttag attaaaaaat aacagtatag atgtgacaat
    16681 aggaggtact tggaatgagt aaaataaatg actctaaaag attagaaaat atagacaagc
    16741 aagtattaat aggaagaaat atcaatctta tttatggaga tgggtgtgaa aagtgttttg
    16801 aaagcacagg atatgaaagc aataatatat gtaagtattg tggttctata gttgcatgta
    16861 ataatgtaaa tttagaactt tatgaaggag aagtccttgg aattgtaggt gaatctggtt
    16921 caggaaaaag cactttatta aaaatactgt ttttccaaga acatgcaaat agtggagaag
    16981 tatatatatc ccattacaat aaagacacaa atatactaga gctttcagac caaaaaaaga
    17041 gatacataaa gaatcatttt atgggtattg tgtatcaaaa tccccacctt ggtttaaatt
    17101 tagactttag tagtggtggt aatatagcag aaaaattatt gatggcaaat ttatataatg
    17161 ttgaaaaaat aagagataga ggcaaagaac ttttaataaa aactaatata cctgtagaaa
    17221 gaatagacca taaaccaaaa tactttagtg ggggaatgca acaacgagtt caaatagcaa
    17281 aagctctttc aaataaccct ccaatattgc tattagatga ggttacaaca ggtttagatg
    17341 tttctgtgca agcggaagta ttggatttaa taagagaaat ccaaagagaa cttaagattt
    17401 ctatgatagt tgtatcccat gactttgatg ttataaagat gcttgctgat aggacaattg
    17461 taatgaaaaa tggaagtgta attgagtctg gtcttacaga ccaaataatg gaggacccac
    17521 aacacccata tactcagcaa cttataaatt cattgctata aatatcgttt gtaaataagc
    17581 aaaatggggg tttttaataa aatgaaagaa actatacttg aagtaaataa ttttagtaaa
    17641 gactttgagt tacatgcatt gaacaagact ataacggcat gtagtaatat aagttttgaa
    17701 gtttcaaaag gtgagttttt aggaataata ggtaaatctg gagcaggtaa gtctaccata
    17761 ttaaaaagta tctataaaac atatatacca acaactggag aaataatttt taattcagaa
    17821 atatatggta atgtagacct ggctaaaatt ggagaccgag aaatgataaa tcttagaaag
    17881 aaagaaatag gatatgtatc acagttttta aagacacttc cacgaataac tgcaattgaa
    17941 ctggtagtac attctttaat tgagagtggg tttgaaaaag attgttcgta tgatatggca
    18001 aaagatatac ttaaccaatt tgaaataaag gaaaatttat gggatgcgta tccaaataat
    18061 ttttctggtg gagaaaaatt aagattaaat ttagctcaag ctatggttaa aaagcctaga
    18121 cttttgttac ttgatgaacc aactgcatca cttgataatc aatctaaaat atatgtaaaa
    18181 gaaaaaatgt tggagcttaa aagtcaagga actacaatga taggaatttt tcatgatata
    18241 gagtttatgg aaactgttat agataagacc tttactatga caaaagggac tataagtgaa
    18301 agtggggtag cctagatgat aaaactaatt cataatgcaa atattgtatt agaagatatg
    18361 attttagaaa atggtcattt aatcatagag ggagatacaa taaaaaaagt atcaaatgat
    18421 aagattgatt acgcatttga ctttaatgag atagatgaaa taatagatgc aaaaaactta
    18481 tatgtaatac ctggtattat agatattcac agtgatgcca ttgaaaagga aatagagccg
    18541 agaccaagta cattattacc atttaatatg gcattttatg aactagataa aaaactacct
    18601 gcaaatggaa taactacagt ttatcattct atatctttag gggatggagt aggagttaga
    18661 agtattgata actctttaaa gatgattaaa aatattgatt catataaaaa tatagatagt
    18721 aagagcataa atcataaagt tcatttaaga tatgaagtgt tgtattatga aggtttagaa
    18781 aaggttttag aactattaga tgaaaataaa atagactact tatcaataat ggaccattct
    18841 ccaggacaag gtcagtatac aaatccaaca ttttataaag agtatgctac taaagtgtgg
    18901 ggagttacag aaaattatgt agatacatgg ttagatgatt tagtaaatct gcatgataat
    18961 ttagattgga ataaaatagc taatattata ggtatagcca aaactaaaaa tataaatgtt
    19021 gcatcacatg atgatgatac attagaaaaa atggatttca taaaaaatat aggcataaat
    19081 gtatctgaat ttcctataac tcttgaagtt gcaaaacatt caaagaaatt gggtgttcta
    19141 acttgtttag gtgcaccgaa tattgtaaga ggtaaatctc ataataacaa tttaaaagca
    19201 atggatgcta tattggaaga ttgttgtgac attgtgtgtt cggattatct tccatcagct
    19261 atgataaagt ctatgtgtat agttgcagaa agaataaata atttaaataa agcagtcagt
    19321 ttatttacct caaatcctgc taaagcagtt ggtatatatg atgaaagagg tagtataaaa
    19381 gaaaataaaa aagcagattt agttttagta gatattgata gtgaatatcc aaaggttgta
    19441 aatactattg taaatggaaa aacagtttat aaaagggagg tataaagatt gctaacagag
    19501 ttacattgtc atacaagtat atcagataat aattttacta ctgaagaaat aataaaaaaa
    19561 gcagcagaaa agaatataaa tatcttagca ataacaaatc atgacacatt atctggattg
    19621 gatgaagctc ttgtgcttgg agagcattat ggaataacaa taataccagg tatagaaatt
    19681 tcagcctatg attataaaaa tagaaaaaga gttcatattc ttggatataa catagattta
    19741 aacagtcaag aaataaaaaa tttgtgcaat ccaatggtat gtgacagaca taaagcatct
    19801 gttaagatgg taaataaaat tatagattta ggatacaaaa tatctctgga agatgtaaag
    19861 aaatattcat caaagactgg aatatttaaa caacatataa tgaaagctct tatagataaa
    19921 ggatatacag ataatatata ttcaaatcta tataaagaat tgtttcatag aggaaacggc
    19981 aaggtatatg tatctttaaa atatgtggat tacagagatg caataaaagc tataaaaaat
    20041 tctggtggaa tttgtgtgtt agctcacccg ggtcaaatgg ataattttag cgctattgaa
    20101 gagatggtaa aagtaggatt agatggaata gaagtatatc atccatctca caacaaagaa
    20161 ctagaaaaag agtcgctttc ttatgctaag aagtataatc tagtggtcac aggaggttca
    20221 gattaccacg gattttattc tcaacataat tatgaacttg gaagcaagag cttaaataaa
    20281 gaagatttat ttaagtttag gttagcttta ggaggggtat tataaatgtc ttttttacaa
    20341 gtaaaaaatg taggtaaaag ttatggacaa gttaaggtgt taaaagacat atctattgat
    20401 attgaaaaag gagagtttat ttgtttacta ggtccaagtg gttgtggaaa gagtacacta
    20461 cttagaataa ttgcagggct tgaggataaa catggtggaa aaataataat aaatgataag
    20521 gatatgacta attcaccacc tgaaagtaga aattttggta tagtttttca atcatatgca
    20581 ttatttccaa atatgaatgt ttacaaaaac atagcctttg gactagaaaa taaaaacata
    20641 agtaaatcta atattgataa aaaagttaaa gaagttctag aagttgtaga attaagtgga
    20701 tatgagaaaa aatatccatc acaattatca ggtggacaac aacaaagggt tgcattagct
    20761 cgtgcgattg ctttagagcc agacttttta cttcttgatg aacctttatc tgcattagat
    20821 gcaaaagtaa gacttaaact tagggagcag attagaagtt tacatagaaa actaggcatt
    20881 acaactataa tggtaacgca tgaccaagaa gaagctcttt gtttagctga taagatggtt
    20941 gttatgaata gaggagaaat aatacaagtt ggaacgccaa aggaggtata taaaaatcca
    21001 gaaacacctt ttgtagctga ttttatagga acaataaatt ttatagatga tggtattaat
    21061 aagatagcta ttagaccaga agatataaaa gtagagtcta atagagattc taaagataag
    21121 gatataaaag tgggagaaat tttagatatc gaatttagag gatttaacta tagaattaca
    21181 gttgagtata gttctaagca aatgaaatta gatgtagttt ccaaagttgc agaacaaatg
    21241 aaactgtgca taggttctaa aataaacttt aagataccta aagaaggaat tgtacaatat
    21301 aaatcagagg gatgtgctta atagctaggg gtgaataaat gataacatca aaaaataaaa
    21361 gaagaataag tgatagcaca atacaaaaat atttcttgat tttttcggta attgtactaa
    21421 tagtatttat attatttcca ttagtatcat tattaaaaaa tgcatttatg gatacaagtg
    21481 gaaattttat aggaatttca aactttagta aatatgttga aaatccaagt cttatagcat
    21541 catttaaaaa ttcaattttt gtatctacag tctcatcttt aatatcacta tgtttagcat
    21601 ttatatattc atacgcaata acaagaacaa acataaagtt taaagatttt tttaaaacta
    21661 tgggaatgtt tccacttttt gcaccaacta tgttatatgg tatttcactt atataccttt
    21721 ttggaaataa gggaatcttt acaaccatgg gtctggaagt accactatat ggtccattag
    21781 gaataattat ttctcagatt ctatttacat ttccacaagc ttttttaata ttatctgtag
    21841 cactttcgat ggctgattat agactttatg aagcagcaga tagcttgggt gctagtgaat
    21901 ttaagaaatt ttgcaaaata acaattcctg gtataaagta ctcattactt agttcattct
    21961 ttgtatcctt tatacttgca tttacagatt ttggagcagc taaggtagtc ggaggaaact
    22021 acaatgtact ggctactgac atatataaac aagtagttgg acaatttaac ataccaatgg
    22081 gagctactgt ttctatggta atgttaattc ctgtactaat agcatttaca ttagataaga
    22141 tggcaagtaa gaaacaaggt atgaccataa ctgcaaagtc tatgccatat aagattaagg
    22201 aaaataagtt tagagataga gtatttttaa tagtatgtag ttttataaca ttgctaattt
    22261 ttattttaat attcgtttca gttataggtt cagttataaa gttatggcca tataatttaa
    22321 gctttacaat ggaacactat acatttaaag gtattttggg aagtggaata aaaacatact
    22381 taagcagttt gaaaatatca actttaacag cagtatttgg aactatattt gtatttttta
    22441 gtgcttatat gatagaaaag gtggataagt ttcccaaatt aagacaaaca gcttaccttt
    22501 tatcaatgat accaatggcc ttacctggct tagttttagg tatatcatat ataacttttt
    22561 ttaactcagc aagtaatcca ttaaattttt tatatggaag tacagcaata ttagttatag
    22621 taaatgtagt tcatttttat tcagtttgtt ttataacctc aaattcatct ttaaaaatgt
    22681 tggataaaga gtatgaatta gttgcaaaat ccataaatat tccattctat aaagtatttt
    22741 ttaatataac agttccaatg tctataacat caatacttga aatagtagta tattattttg
    22801 taaattcaat ggttactgtt tcagcattaa tatttttata tacacctcaa actcagactg
    22861 catcaatatc aatactaaag ttagatgaga taggatatat aggaccatca gctgctatgg
    22921 cagtattagt gcttttaaca aatataatag taagactttt gtacgaattt gtaacaaaga
    22981 aattaaaaaa taacacacaa aaatggcagc aaaaagatgt tgcatagggg aggtaaaaat
    23041 gaaatttaag aagctggttt ctttaggagt attatcaaca cttttattaa gtgtaataac
    23101 aggatgtagt actgattcat caggttcaga ttcaaagtct gatagtaaga caaaagggga
    23161 tttaacagtt tacacagcaa ttgaagaaga tagtatagag ccatatttag ctacgtttaa
    23221 agagaaatat ccagatatta aattaaatat tgttagggca tcaacaggag atataacagc
    23281 aagattatta gcagaaaagg ataatcctca agcagatgtt gtatggggag ttgcagccac
    23341 aagcttattg gttgcagatg accaggggat gttagaacca tatgctccaa aaggttctga
    23401 agaaatagag tctgcattta aagatgataa agaagttcct tcttgggttg ggatagatgc
    23461 ttggatgaca ggtataacag taaacactaa agaattaagt gataagaaca tcccagttcc
    23521 tagttcttac gaagatttaa taaagccaga atataaaggt cttatatcta tgcctaatcc
    23581 atcttcttct ggtacagggt acttaacagt atcagccctt atacaaatta tgggggaaga
    23641 aaaagcatgg cagtatatgg ataagttaca tgagaatatt ggtgtataca ctcaatctgg
    23701 ttcagctcca gctacatcag cagcttcagg agaatatcct attggtatat cttttggata
    23761 tagaggtata aagttaaaag aggaaggcta tccagtagaa gtagtatttc caaaagaagg
    23821 ttctggatgg gatatagaag caaatgctct tgtgaaaaaa gataatctaa aagaagcatc
    23881 aaaagtattt ttagattggg ctataagtaa agatgcaatg aacgaatact ctaagaacta
    23941 tgcagttact actatttcta caggaaatcc aataccagaa ggattcccta aaaagccatt
    24001 agagcaaatg atagataatg atttaaaatc agcagcaaaa aatagagaag atatactaaa
    24061 taaatggata tcaaaatatg atggtaagac agaaaaagag agttaagtaa aactatacaa
    24121 atggagtaaa aagaaagaat ttttttaagt agattttatt ataataatat aagttgagaa
    24181 taatataggt caagaaaagt gaatattcta taaaaatgta tagagtaagt ataccattca
    24241 gtttgaatgg tatatttttt atttttagag attaatttag aaatagatat actacagttt
    24301 aaaatagtat attttaattt cattattaaa gtatagatat tatatataaa gtaaataaat
    24361 tattgagcga ttattaataa gatatgtata aattttaatt tgggaacttg tgttatatat
    24421 catgaaaaaa gagaaaaaat aaaaggtaaa ttgtttcttg ataacttgaa attggaagtg
    24481 agaatattta actagagaag aattatttaa gtttaagtca gctttaggag gaggaattat
    24541 aaatgtcttt tttacaagta aaaaatgtag gtaaaagtta tggacaagtt aaggtgctaa
    24601 aagacatatc tattgatatt gaaaaagggg agtttatttg cttattaggt ccaagtggtt
    24661 gtggtaaaag tacattgctt agaataattg caggacttga ggataaacat gatggaaaaa
    24721 taataataaa tgataaagat atgactaact caccacctga aagtagaaac tttggtattg
    24781 ttttccaatc atatgcacta tttccaaata tgaatgtata taaaaatata gcctttggat
    24841 tagaaaataa aaatataagt aaatctgata taggtaagaa ggttagagac attctagaag
    24901 ttgtagaatt aagtggatat gataaaaaat atccatcaca attatcaggt ggacaacaac
    24961 aaagggttgc actagctcgt gcgattgctt tagagccaga ctttttgctt cttgatgaac
    25021 ctttatctgc attagatgca aaagtaagac ttaaacttag ggaacagatt aaaagtttac
    25081 atagaaaact aggcatcaca actataatgg taacacatga ccaagaggaa gctctttgtt
    25141 tagctgataa aatggttgtt atgaataatg gagaggtaat acagattgga actcctaaag
    25201 aagtctataa aagtccaaaa acaccttttg tagctgattt tataggaaca ataaatttta
    25261 tagatgatga tgtgaatatg atagcaatta gaccagaaga tataaaggta gaatttaata
    25321 gagactttaa agaaattgat gtaaaagtag gagagatttt ggatatagaa tttagaggat
    25381 tcaactatag aattacagtt gaatattgtt ctaagcaaat aaagttggac gtagtttcaa
    25441 aggtagtgga acaaatgaaa ctatgtgtag gttctaaaat aaacttcaag atacctaaag
    25501 aaagaaccat acaatataaa tcagagggat gtgcttaata gctagaggtg gataaatgat
    25561 aacaataaaa aataaaagaa gaataagtga tagtaagata caa
//
LOCUS       DAEORZ010000046        25164 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEORZ010000046 DAEORZ010000000
VERSION     DAEORZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25164
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.046"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(149..1027)
                     /locus_tag="KRH39_001724"
     CDS             complement(149..1027)
                     /locus_tag="KRH39_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG8511637.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(1044..1361)
                     /gene="trxA"
                     /locus_tag="KRH39_001725"
     CDS             complement(1044..1361)
                     /gene="trxA"
                     /locus_tag="KRH39_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG8511638.1"
                     /translation="MAKVINTSEFRSSVEGSKGIVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1511..2743)
                     /locus_tag="KRH39_001726"
     CDS             complement(1511..2743)
                     /locus_tag="KRH39_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8511639.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKNPKDLVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKKFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKSGDEIVLELREDIEDEV
                     ILLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIEINVT
                     SDIDIGTTFELSIPKKLK"
     gene            complement(2733..3404)
                     /locus_tag="KRH39_001727"
     CDS             complement(2733..3404)
                     /locus_tag="KRH39_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8511640.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FLLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3420..4256)
                     /locus_tag="KRH39_001728"
     CDS             complement(3420..4256)
                     /locus_tag="KRH39_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511641.1"
                     /translation="MNKIVKKSISLMIILTIFIFTLTACEKDEQPDSVDIQAEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKSVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4282..4956)
                     /locus_tag="KRH39_001729"
     CDS             complement(4282..4956)
                     /locus_tag="KRH39_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG8511642.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDAGLKNAKPKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4970..5698)
                     /locus_tag="KRH39_001730"
     CDS             complement(4970..5698)
                     /locus_tag="KRH39_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG8511643.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKTLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5825..6514)
                     /locus_tag="KRH39_001731"
     CDS             complement(5825..6514)
                     /locus_tag="KRH39_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8511644.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNYFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6522..7085)
                     /locus_tag="KRH39_001732"
     CDS             complement(6522..7085)
                     /locus_tag="KRH39_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG8511645.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPI
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(7087..8055)
                     /locus_tag="KRH39_001733"
     CDS             complement(7087..8055)
                     /locus_tag="KRH39_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBG8511646.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8156..8199)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8564..9193
                     /locus_tag="KRH39_001734"
     CDS             8564..9193
                     /locus_tag="KRH39_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8511647.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            9168..9572
                     /locus_tag="KRH39_001735"
     CDS             9168..9572
                     /locus_tag="KRH39_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBG8511648.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9575..10051
                     /locus_tag="KRH39_001736"
     CDS             9575..10051
                     /locus_tag="KRH39_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HBG8511649.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            10598..11263
                     /locus_tag="KRH39_001737"
     CDS             10598..11263
                     /locus_tag="KRH39_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBG8511650.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            11285..12217
                     /locus_tag="KRH39_001738"
     CDS             11285..12217
                     /locus_tag="KRH39_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBG8511651.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKSATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            12238..12879
                     /gene="pcp"
                     /locus_tag="KRH39_001739"
     CDS             12238..12879
                     /gene="pcp"
                     /locus_tag="KRH39_001739"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBG8511652.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(12999..13331)
                     /locus_tag="KRH39_001740"
     CDS             complement(12999..13331)
                     /locus_tag="KRH39_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8511653.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            13596..14216
                     /locus_tag="KRH39_001741"
     CDS             13596..14216
                     /locus_tag="KRH39_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8511654.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(14541..15365)
                     /locus_tag="KRH39_001742"
     CDS             complement(14541..15365)
                     /locus_tag="KRH39_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBG8511655.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(15376..16779)
                     /locus_tag="KRH39_001743"
     CDS             complement(15376..16779)
                     /locus_tag="KRH39_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8511656.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDDGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(16783..17346)
                     /locus_tag="KRH39_001744"
                     /pseudo
     CDS             complement(16783..17346)
                     /locus_tag="KRH39_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(17451..18491)
                     /locus_tag="KRH39_001745"
     CDS             complement(17451..18491)
                     /locus_tag="KRH39_001745"
                     /inference="COORDINATES: protein motif:HMM:NF016362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HBG8511657.1"
                     /translation="MSYKINFDNLTDTEFENFCFDLLKGLKCEDLNWRKGTGKSSSPA
                     DGGRDIECTYIKMDYLLGETNIEKWFVECKHYIKGVPADKLLGALTSAMSMDINRCVI
                     IVSNFLSNPAKDMLNNFIEKNRPKFKISIWERPKLEVLTKNMDYLLRKYNIMYKDDIL
                     DYMNPYHIEYIKFPYLNKLSSFLEPFNKLDKNTKNEIVNLLSILYLGEYLETRPSVFS
                     NYYDMYNELLKKFIKVSDITSEHVVVNSITSIILNFFLSSGNPSSVDKEINDSERSLL
                     NIEKDKEKIIKRAKENVGDIEVEEEEYINGIKLIKELTQNRILNLERWSKERYSIYNE
                     FCNVVVRNLIYS"
     gene            complement(18572..20029)
                     /locus_tag="KRH39_001746"
     CDS             complement(18572..20029)
                     /locus_tag="KRH39_001746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511658.1"
                     /translation="MSGQSALRGYVYQGLALIIESLEDKNWTHIALEFPTEQDKVDIA
                     LFKDSTLIKVIQVKSSINLFNKEDIKKWIRELIDDESSSKEYKLYLIGQCRNNANIFI
                     KSVDKYNNRIIDEESKKSLNEFTSILENHKVSIDVIPFNKKYILGYIGDKLNKYLSEK
                     DIILDPKKIDLAIDSCLGKILLLGTSSSPLTREELDDIMINFVMNSFEEKSNKISNKK
                     YKNYIIFMIIPLILNLSQTLNFVNSNYIYITISLVLLLIVHYTFKISDNEFKKIIKPE
                     NMCNYTFNETPYIRVNVSLDNLMNEQIINIENISDNDITEIKGNINFFNNNCRVNTVR
                     FNDFNIGRNRIEVINCINNKLGKEYYKRIFWNEVELNIEYIKTPDINVGFDKYTLYRN
                     KRTYYNELNYYNYYYIGKFRIPYEITWLRDNFIPKIKSYIRFSSKNREIKKLVVILFS
                     TIAIAVLIIGSSIGLSKIVYFYIELLKNILVYFSH"
     gene            complement(20198..20899)
                     /locus_tag="KRH39_001747"
     CDS             complement(20198..20899)
                     /locus_tag="KRH39_001747"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511659.1"
                     /translation="MNIKNLGCDFEKLLRQYNEASELIKTLKLYGELLLFGGAVREYM
                     DNKFNHLPRDFDIVIKKNNEYVNLDEILYKFQYKKNRFGGYKIRVNQIEFDIWEIENT
                     WAFKENKVQINFGDYEKKLQDTVFLNIDSIVYNLNTQDYYNKKYENAMENKSLDVVLE
                     ENPYIELNLLRAILFKKKYNMDFSDKLSRILSDFVDNNNNYLKLMEEVQIKHYRTFKI
                     ESLDLDKEINSIIYY"
     gene            complement(20920..22398)
                     /gene="dgt"
                     /locus_tag="KRH39_001748"
     CDS             complement(20920..22398)
                     /gene="dgt"
                     /locus_tag="KRH39_001748"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HBG8511660.1"
                     /translation="MEQRLDWKKLLCGERVRASSTPINQNLNINDEGYNHRNEFDKDY
                     DRIVYSSSVRRLQDKAQVFPLQENDFTRTRLTHSLEASALARSFGYAIGEWLLSEGEF
                     ENYKQVRELSALLQVSALIHDLGNPPFGHHGEEVIRNWFKEWLKIEKNGDGLNEQQRA
                     DFENFEGNAQTLRIVSKLQLLNDEYGVNFTYATLATIMKYPWKSNSEKGKAESKFGYF
                     SAEEKLVEDVFDKVGLKEGIRHPVTYLLEASDDIAYLLADVEDAVKKGILPWDKEYKK
                     IKKALKENEKEEESKILYEKMFKSLDEQIERNNKNEIPESNLANIQNFKVLAQGMMFN
                     QVVNMFKENYEDIMLGKYGIENLLKKSKVSELIKEVKRLAKEYCFVNKEVLTLELVGD
                     RVINGLLDTFVDSLVGNVGEINHKEKEGKLYYLISDNFKYICCLDEEKNFNRKKDELM
                     RQIDFKEVKKYNKLLLITDFISGMTDSYAVNLYKELMGVKLP"
     gene            complement(22842..23653)
                     /locus_tag="KRH39_001749"
                     /pseudo
     CDS             complement(22842..23653)
                     /locus_tag="KRH39_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(23655..24052)
                     /locus_tag="KRH39_001750"
                     /pseudo
     CDS             complement(23655..24052)
                     /locus_tag="KRH39_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620182.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
     gene            complement(24224..24463)
                     /locus_tag="KRH39_001751"
     CDS             complement(24224..24463)
                     /locus_tag="KRH39_001751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511661.1"
                     /translation="MEIGRRIIFDQDGEIIAIYGETEGDVIPRKGISKIDYIDIPFNS
                     IPDNCYIEKIDTINNVPVIKRLKIELTEEEKEYKS"
     gene            complement(24478..25146)
                     /locus_tag="KRH39_001752"
     CDS             complement(24478..25146)
                     /locus_tag="KRH39_001752"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511662.1"
                     /translation="MLEKLNENASLSELITTFGDVKNELQISKNNIASALGSPFAGTD
                     KLDVTKTKIETLKNTFATNLTNKNVSTAGSESMQSLIDKVSSIKNIIKTAKSGNSLLR
                     LQKAYIENYNSEQVWVWGAFTVDSLAFKPNLVFAQAGNSRNKYTIVSFDSSMELKSNL
                     IFCTNSQSIPSTRYSIYHVKNREMDVDNGSSVGHFSGGFRLPIPNEDFDSSATISWFA
                     LGYK"
BASE COUNT         8494 a         4013 c         2616 g        10041 t
ORIGIN      
        1 actttgaatt tttttaataa aagtaattac atatagattt taaaatactt ttattaataa
       61 tttaaaaata aaaaactgtc ttgaaataat aaaattattt caagacagtt ttttataaat
      121 atctttaaaa agttaatcaa tataaatatt aagccctctt taatttatct aaatagtaaa
      181 cagcatttaa agctgctatt tgcccttgac cagctgcttt tatataagaa tagggttttc
      241 caacacaatc tccagcagca aagcatccat ctatactagt cttcatatta ttgtcaactt
      301 taatatgaat gccatcaatc tctattccag gaaccaatgc acttggagcc gtactatctt
      361 ttataacgaa tactccatca acttcaatgt ggttttcttt aaaagatact ttgtttacaa
      421 gtttatctcc atctatctgt actggtctgt catgtataac ttcaatagaa tcatctaggt
      481 tatctgaact tctcataaga ttatcttttt tgtacatagg tataaaataa gtctttgaag
      541 tgagttcact taaaaaattt gcttcttctt tagattcttc attatatcct ataactgcta
      601 cctttttatt tctataaagt ggtgcatcac atgtagcaca atagcctaca cctcttccta
      661 gaaattcttc ttcacccttg attggtctag tatactctac cccagttgca aggattacag
      721 tagtagcttc atacatatca tttccagaca ttattgtaaa gtattctccc atagcataaa
      781 tattatttat tcttttattt tcaattgaaa tatccatact atctatatgt gatttaaact
      841 tgtcttttaa ttcatcacca cttatatcat aaaagcctaa ataattatct attgaagggg
      901 ctttaacaag cttattactt aaattatcat taccaaacat gataattgtt ttatttctta
      961 tttttgcatt tatagcagca gacaatccag ctggaccact gccgattata gctatatcat
     1021 atctcataat actcacccat tttttataaa tatgatttta cttgcatttc aattttgttc
     1081 tttggcatga atccaattaa agtttctact ggttttccat ctttaaatat aatcatagtt
     1141 ggtactgtag atacattgaa ttgttgagct atttctaagc tctcatctat atcaaccttt
     1201 acaaatctag ctttatcttt catctcttca cctaattctg caaatacagg tcccaacatg
     1261 ttacaaggtc cacaccaagt tgcaaagaaa tcaactacaa ctattccctt actaccttca
     1321 acactacttc taaattcaga tgtatttata acttttgcca tttttaaatc ctcctattaa
     1381 actttattat ccctatataa ttatactatg aaatttataa atataaattt acttttataa
     1441 ggtcttttta tattttatac taattagtat acccattctt catttctaaa aacaattttt
     1501 tatattttta ttattttaac tttttcggta ttgacaactc aaatgtagtt ccaatatcta
     1561 tatcagaagt tacatttatc tcaatagata agtttaagct aatcatacga gctattgaaa
     1621 gtcctattcc agaaccttct aaatctttat ttcttacatt ttcgcttcta aaaaatctat
     1681 caaatataag aggtatttct tcttttttta tgcctatacc agtatcactt ataagtaaaa
     1741 ttacctcatc ttctatatct tctcttaatt ctaatactat ctcatcaccc gattttgtat
     1801 atttaaaagc attatcaatg aaaatcaata taagctgtct taatttattt ttatctgtta
     1861 ttataacttt attcttcagt ttagaattaa acacgaattt tttttcctga aactcagcaa
     1921 tgtcaatata gtcttctgat atttcttcca ataacttttc taagtctatt tcttctaaat
     1981 taactttaac aatagagtcc tctttagtaa gagacaataa atcagttatc atttttttaa
     2041 gtcttctagt ttccttcatt gcagtagcta ttgtttcaac ttcatcattt actgtacttt
     2101 ctggtgattt taacatactt tctaattttg atgaaactat tgttattgga gttcttaatt
     2161 catgagaagc atcttgtata aacttagctt gatttttcca tgctgtttct attggaatta
     2221 aagcttttct agtaagatac acagccacaa agtaagtaat tattacagaa attaatattc
     2281 caattataag aacagatgta agctgtctta aagaattcat ctcggaatct atatttctaa
     2341 ttatttgtat ttggtatcta cctacatcta catttaattc tctaaaagta tatccatttt
     2401 ctgtgtattt aaaaaacgaa ttttttttat ctagtcttct atctggaagc aactcatcaa
     2461 aatattcatt ttgagtatag taacttatcc ttccatcttc atatacatac accaaatctt
     2521 tagggttttt gagtcttatt gggttcaaaa atgaagttct tttaaattga gaaactatat
     2581 attcaagttc atcatttatc tcactatcaa ttccactata tgtaagtccc ctaaaatatg
     2641 agtatataaa tatagagaag attattaaaa aactacctac tacaataata tacattttta
     2701 ttaaatgatt tttagtactg gtaaatacat tcttatttat catcgaatat atatcctacc
     2761 ttacgtttag ttttaatata tttatcatat ccatatttac ttagcttttt tcttagatta
     2821 cttacatata cttccactat ttcaatagtt gcatctgaat caattcccca tattctatca
     2881 taaatctgct cttttaataa aattgaacct ttatttaata gaaaatactc aagcaagtta
     2941 aactgtttgt tttgaagttt tatttcttct ttttcaatat acactctttt ttctagtgta
     3001 tctaaatata aagatttaaa ttctaatccg tttctttctt ttatctttcc attagtcctt
     3061 aatatagcgt atacccttgc tactaactct tccatataaa aaggttttgt caaataatca
     3121 ttagctccta tagtaaatgc ttctactttg tcatctaatg tttcttttgc tgtcaatata
     3181 agtacaggtg tatctatatt attaactctc attttcttta ggatatcaaa tccatttata
     3241 ttaggaagca ttaagtctaa aataactaaa tcatatatat tttgatttac taagtataaa
     3301 gcttcttctc catcttcaca cttttcagtt tcaaaatgct tgctaagttc ttctactaca
     3361 ctttctaaaa gtatcttatt atcttcaact attaaaacct tcataagtat cccctacctt
     3421 tatttgtcta tcttaacttt atgagatttt aagtcaatat ccacattggc tttttctttt
     3481 atatcatcat acgtatatcc cactatttca acactttttg cctttgttga gcacaatata
     3541 tctatatctg tctcttctga aggctttaaa atcttatatg atggcacacg atttactgtt
     3601 aaatattctc cattttcatt aacttctgct gtcttaagaa taatatttcc taaatctttt
     3661 gatgatgaat tttttacttt tacttgatac gtaattccct catttgattc atttactttt
     3721 ttaagttctg atgtagatag cacctcatac tgactactat tttctagctt aatattatta
     3781 tttcttatat taatagtatt ttcttttaaa ttaacataca caatcttacc ctctgcctca
     3841 tattcatatg cataaacttc tacactcttt acaaaagttt tatgcccaca ttctatgtaa
     3901 gcaactttcc caggcattaa agtcatgtcc aacaaagcct tactatctga agatataatt
     3961 tttttattct catcatattc attatatatc aattcaacat tggatatatt aaatgttgaa
     4021 ttattttcta cctttgttga tgtatctact ttatcacctt catctatatt tataatttct
     4081 gattttccta tattcaatac ttttgcattt ccctcatcta tttttatctc atttttatct
     4141 tctgcttgaa tatcaactga atcaggctgt tcatcttttt cacatgctgt caacgtaaaa
     4201 ataaatattg ttaatataat cattagagaa attgattttt ttactatttt attcatatta
     4261 agcacctcat atagtagtat tttatagtag atttttatac tttttcataa attctttcaa
     4321 tgcctgtgta agtatttgat gtttttcaaa attcgaatta ctacataatc tttcaaaatc
     4381 attccatatt tctttatcta tacgtattct tgttgttttt aattcatcta tgtgttcatc
     4441 atctatatgt acatcttttt tattagatac ttttttaggc tttatattgt aatcttttac
     4501 ttctaaatac caatcatata cttcacaaag cttatctaaa tcttcttgag ttatatttat
     4561 ttttctatct ttcttaggct taacattttg ttttggcttt gcatttttta aacctgcatc
     4621 attaaactca atttgttcaa cacttttact taaagccttt tcattagaaa tctttttttt
     4681 ggtgcttact ttttttctat tacttatagt attcttttta cttttttcat actctttagc
     4741 tttttcttct tccaagtttc tattatctaa acttttatta taatttaatt cgtcttttaa
     4801 tatgtattta cctttttctg acttatatcc ttttttattt aaaaaagttc ttaaagtact
     4861 ttgactaatc tttagatttt gagctatctc tgcaaattta aatcctttgc tttgaagttc
     4921 taaaaatttg tcaattctgt cttcattgtt attcattaat attcctccgt caacatcttc
     4981 ttgtctttct tttcatactt cttattatat tcttcttttc taaatctact ctatttgatt
     5041 caattatttt ttgtagagtt ttattataag tgaaatcatc taaattatta ttttttatat
     5101 agagaaaagt ttcctcttca catttaataa aacaaattga tagcaaccat gccataccca
     5161 ttttaacata atattcttca ctttgtatat tattacagta ttcaaatatt tctttaatat
     5221 gtttatcatc aacataatat attaaaaaca taactaatgc aaatctaatt tcccatggat
     5281 tctttgaata aacatatttt ttaagtatat catacatatt ttctttatat tttttcatag
     5341 actttaaatc agaacaaaat ccatcgcaaa ctgaccaatt atcgatttta ggtataaatc
     5401 tttttacata gtataaagtt tcttcaaaat tactatttat attgccaatt accatacctt
     5461 gtagcctaac ttcttcatag tatgtatcct ctgctacttc taaaaattcc ttccaatttc
     5521 cttggcttat ttcttttgat aactttctta aattaggaac tctaatacct attatatttt
     5581 ctgttccagg tataagcttt ttattaaaat ctctatattt tatatcttgt atagacatca
     5641 tatattctaa aagttcatta taatccaatt tattccattt tcccctatca aacatcattt
     5701 aattgctcct taattccttt tttcttattg taacataaaa ctctacaaaa aaagcttcta
     5761 actattagaa gctttttcaa aaacatagga aacttttcaa ttttaaattt aaactaaatt
     5821 aatgctaatc tcctacacca aagtctgtat tattgtataa aacttctata tttggatttt
     5881 catccaagaa tttatactta ctaacaaacc atttcactaa ctcttcatct ctttttatac
     5941 tagaagaatt atttataaaa acattcactg tcgctttttc aaaagtattt aggataatct
     6001 ctatatcttt atctatcatc tcttttgttt gtccttttat tccaaccata atacaaggtg
     6061 aatcaaaata ttcttttaca tcttgtggag tcttaaaatt tgcattttta tttaaaatat
     6121 tatttctaaa ataattgtca aaggtttcaa ctccaatttt aaataccaca ggaatttcaa
     6181 aatactctct catttcctta agcctatttt tgtaagacca atgactttct aaaaataatt
     6241 tttttatatt tttctctttt ataatacact ttattttttc taaagtatcc ttaggtaatt
     6301 caaaacagct tccagaattt atgacttcaa gaactccata tttcccagtt acgtttttta
     6361 aaacctttag atttaattta ttcatctctt cttctaaatt agaattatca tctatataat
     6421 cacaaaaaga acactttccc catatacaag gaaatccttt aagcagaaca atttctcttt
     6481 gatttttatt tgttatctca ctatatctat ccattttcta tctattttcc tccagtcaat
     6541 gataccttta aagtttttgg aactgcattt aatcctaaat tcactaaaac aactgctaac
     6601 agcttatctc cataatcagt aaatacttgg gttacaaaca cactaaaaac atctcccagt
     6661 cctaacactt tcaagatttg cactatatat gatgaacctg aagaggtcat acctccaaat
     6721 aaaaatgctg aaattattga acttattata gatgttggaa tggcaaataa aagtactcct
     6781 attggagttt ttaaacccct taaaaaattc tttttaaatg ctattccagc aagtaatccg
     6841 gttgatattt gaactggtgc aaaatagaga gaatacacat caaacgtaat tccacttatt
     6901 aaacttccaa acacacctgt aacaacagcg tatttaggtc ctaaaagtga agctatcatt
     6961 atagttccta ttgaatctaa gtatacaggt aatcttaatc ccatagctat aaaagcgcct
     7021 attatattta tagaaatacc aaaagctata agtgtaatag tttttacaca tatttttctt
     7081 ttcacactat atcactcctt ccacagagtc tataatgtct tcataacctt taaaaagcct
     7141 ctttaaaaac atgtacataa atttcttctc atcaacttca tttagaacaa ttgcattagg
     7201 atttttttta tagaaattga aagaatccac tatagactga cccatagcta ttccatcttc
     7261 tacaacttct acatatgatt caaatccttt acatatactt ctatctataa agtatgctac
     7321 tgctagaggg tcatttatca cacagccaat tattccctcc tgttcccaat ggaaatcaat
     7381 ataaaatcta gttatttcag ttatatactt tgccatcttc ttatcaagtc tatttataaa
     7441 ctcaataata ttaggagtaa gtacaatttt tctagttaca tctaaaccta ccatgtggat
     7501 ttttttagat aaattcttgt aaacataatc tgctccatgt gggtctaccc aataattaaa
     7561 ctctgctact ggagagcaat ttccatgaat cctaaatgct cctcccatag atacaaactc
     7621 atcgagattt tcaaatgctt tcttatcttt aataagtgct ttagctatat ttgtaagtgg
     7681 tgcaagtgct attattgata ctttttcatg attatgtaaa gtatttatta taaaatccac
     7741 tgctccattt tttgctttag ctcctacaac cttttgataa aagttttctc caataccatc
     7801 ttctccatgt gtatcttgag ctgttacaag ttttcttttt agtggtgctt cttctcctat
     7861 atatactgga atatttagtg aagaacacat ttgaagtgtt tttagtgcat tttctgcacc
     7921 tatatttgct ggaacatttc cacaacatgt ggtaattcca attacttcta gctctggtga
     7981 gtttaaagcc agaagaattg ccaaagaatc atcaattcct gggtcacaat caattattac
     8041 ttttctcttt tccatttttt atcctccttg atttgtggtc gaggagaact acacatcttt
     8101 tttccacttt caaaaaggtg tgtctaaggg tattaattat atactgccac tatataattc
     8161 cttagttttt tatacttgga gttctcgaac aagtacagat gtaaaatcat atttttaggc
     8221 tacttactat agactcttaa aaactataga ctgttaaaaa atttttaaac atctgattta
     8281 atagctataa aataaatttt atagcataaa agaatttatt atattttaat gtatcttatt
     8341 aaagtttatt tagaattatg aaccaacttt aataagatgt ggcgcattta aaccgattat
     8401 tattatacaa taatatctta aatattacaa ttaataacat taatttatta tccataaaaa
     8461 tatattaact tatattattt aataatttat tgacagtatt aaaattaaat tatacaataa
     8521 cagtgtaaat taacttaaca ccgttaagca aagaggtgat tatatgagta tcaaagaaag
     8581 acgtaaaaat gaaaaagaag aaatgaagaa aaagattatg gatgctagca tcgaaattat
     8641 aaaccaacat ggatatgaaa atttatcaat aagaaaaatt gctacaaaaa tagaatattc
     8701 tccaactaca atttatcttt actacaaaga caaagcagaa attatcagcg atatgaccaa
     8761 taaactttat aacacagtag aaagtaatgc tattgacatt atgaataatt gctcatcact
     8821 accaatagat aaacaaatca tagaaattat gactagcttt ataaaaactc tttctagtga
     8881 accagagatg gcaaaatcta tcatgcatag tagaatgaat attatatttg catctgaaaa
     8941 tacaaacaat acgcctagca ataatggtat aaaaatgctt gataaattcc tttccattgg
     9001 aattgaacaa ggaatattca aaaaaaatat agataaaagc tcatggatgc ttataagtgc
     9061 attgttagga ttcgttttat gtgttgttga aaataaacta tattctttac ataatttttc
     9121 tcaacttata gaaaatttct tagatatttt agtgggagga ctttacaatg aaaattcgca
     9181 acaaggctac taataaaaag aaaaaatatg taaaaatatc tattgctcta ttctttgtat
     9241 ttatatgcat tattattgta attgtaagca acttaaagcc agaaaacttg gtagtaaaag
     9301 atatagatat taataatgtt aaaaatggca tttatactgg aagtgctgat aataacttgg
     9361 taaaagctac tgtgtctgtt gaagttaata atggtaaaat ccaaaatatt aatatcttaa
     9421 aacacgacca tcttttaggt aaacctgctg aaaaaattac aacaagtatt attaaacaac
     9481 agtctttaga tgtggatgct ataacttctg caacttatag cagtaatact atacgaaaag
     9541 ctgtagaaaa tgctttaaga aagggagaat aattatgaat actattatta tatattcaag
     9601 taaatatggt tgtacaaaag actgtgcaaa tatactaaaa aataaactat cagataatgt
     9661 cactattgtt gatattaata ataataataa taaaattgag ttatcaaaat ttgataaaat
     9721 tattattgga agttctatat atgttggttc agtttcaaaa aaaatacaag tgctatgtaa
     9781 tgataatgta gagttattaa acaaaaaaca agttggcatt ttcctttgct gtggcttttc
     9841 tgaacaagca gataaatatt taaaatcaaa ttttccatca tccttattag aaagtgcaaa
     9901 tgctattgga atttttggta gtgaagctcg attagaaaaa atgaaatttt tagataaact
     9961 tattatgaag gctgtttcaa aaggtaatta tgacagtttt agaatatcac aggataatat
    10021 agataatttt ttaataaatc ttaatagcta aaataaagct aattatttct atgataaatt
    10081 cttttattta taagtaatat tgctcaaaat agtctttaaa ctttatcaaa attttatttt
    10141 tcttagtgaa atctaatgaa actatcactt aatatatgct ttattaatca taacaaaaag
    10201 agagtgtcta atgaactaaa aaattcatta gacactctct ttttgtatga aatcaaatat
    10261 atttttatca tttcaaaagt gaaaaataca cttgtctcta tgtttggcac attctctttt
    10321 tataatataa tatctaattt aaaacctatt tcaaatattg acatatactt aaactataga
    10381 cttacaaaat caattcatat tagcttaaaa tataatcttt gttattcaaa ttcctataca
    10441 caaccattat ttactttgat actttaaaac atcttcccta ttttttatta aattttataa
    10501 taatagtcct attaacgcaa atactataaa aagagtttaa ctttatccat taaataaatt
    10561 taaattaaaa catatagaaa tggaaggaga acaatttatg ataaaactaa tcggtatatt
    10621 gatagttgta attggattta tacttaaaat tgacacatta tttactgtat tattagcagg
    10681 agtggcaaca ggaattgttg caggattaga ttttaatcaa atttttacaa ttttaggaga
    10741 ttcttttgtc tcaaacagag gtgtatcatt attcatatta acattgccag taatcggtgt
    10801 attagaaaga tatggtttaa aacaaagagc tgtttcttta atagaaaaat taaaaagact
    10861 tacaacagga aaagtactta ctatatacat gatagctaga caaatagctg gtgctctatc
    10921 aataagaatg agtggacatc ctcaatttgt aagaccactt gttaatccta tggcacaagc
    10981 tgctggttta agtaatagtg atgagctaaa agaatcggat gaagaagcca taaaggcatt
    11041 atctgctgca tcagaaaact atggaaattt ttatggtcaa aacttatttg caggtagtag
    11101 tggagtatta cttatagcat ctactctgac tcaatttgga tacaatgtaa ctggacttaa
    11161 tattgtaaag gctagtacta ttatggcagt aatagcactt gtagtggcta caatccagtt
    11221 taccttatat gataaaaaat taaacaaaag tcataaaaaa tagttttatt taggaggaat
    11281 aaaaatgcaa aattttataa acatatcatt agaaatcttt tacgcactaa tgggattttt
    11341 aatgattgtt atagcatata agtcttttac taccataaat aacaataaga aatatggtac
    11401 atcattattt tggatactta tatcactgcc attcatattt ggtagattga ttccagccaa
    11461 tataatagga ataatattaa tattatctag tttattaaca ctttctaaac aagttgtatt
    11521 tgcaaagtat gaagaaccag atgaaaattt tggaaaagaa caagcagaca aacttaaaaa
    11581 taaaatattt ataccatcat taatattagc atttgctgca gtagtagtag ctatgagcct
    11641 ttctaatttt gataattctt ctcaatttgc aataggtgtg ggttcaataa ttgcattaat
    11701 atcagccctt ataataacta aggcaaaatc tgctacatca gttcaagatg gctcaagatt
    11761 acttcaacaa atgggacctg ctagtatgtt accacaactt ttagttgcac tgggagcttt
    11821 atttactcaa gccggagtcg gtgaagttat ttcaaccatg atatctggtg tagtccctgc
    11881 tgatagcaga ttatttggtg ttatagctta tgtattaggt atggttatat ttaccatgat
    11941 aatgggaaat gcatttgccg cattttcagt cataacggca ggaattggta ttccttttgt
    12001 attgtctcaa ggtggaaatc ctgctataat tggagcatta gcattaacag caggatattg
    12061 tggaactcta cttactccaa tggctgctaa ttttaatata gttccagcag ccttgctaga
    12121 atgtaaaaat gattatattg ttataaaata tcaagctcca gttgctttgg tattaattat
    12181 agcccacata ttagtaatgt actttttagg attttaatat aattaggagg taaaataatg
    12241 aaaattttgt taacaggatt tgacccattt ggtggagaac caataaaccc agctcaagaa
    12301 gcagttgaaa gggtcaataa taatataaac ggtgcagaaa tcataaagat aactatacca
    12361 acagttatga caaaatctgt tgaagctata gataaagcta ttcaagagca caatccagat
    12421 atagtaatat cagttggaca agctggaggt agatttgaca ttacacctga gagagttgct
    12481 ataaatatag atgattttag aataaaagat aatgagggta accaggttat agatactatt
    12541 ataaaagaag atggtgaacc tgcatatttt tcaaaattgc ctgtaaaagc tatggtaaaa
    12601 catatgaatg aaaataaaat tccagctagt gtttcaaaca ctgcaggaac atttgtttgc
    12661 aatcatgtga tgtatggaat tctttatatg atagacaaga aatatcctaa tataaggggt
    12721 ggatttatac acatcccata tacaacttca caagtaatag ataaaaaaaa tacaccattc
    12781 atgtctttag aagaaatagt aaaaggctta gaattagcta tagaagcttg tattatatat
    12841 aaagaagatg taaaagaaat tggtggtgaa atttcataat aaatttttta gtatttttat
    12901 aaattaaaaa tactaactat taatttaaat atagactgtg agtagattat tcaaacaatt
    12961 ttactcacag tctttttatg gtatctacag atacaaaatt aaggtctatt cctctctccc
    13021 cactcacaca ttgcatctaa aattggaatc agagattttc ccctttctga tagactatat
    13081 tctaccttag gaggtatttg aggatattca gtacgaataa tcaagttatc tgcttctaat
    13141 tcttttaatg ataaactcaa agttttatag gagatagtat ttatataacg ttttaatgca
    13201 ttatatctta caacttcaaa ttctacaaga cagtaaagaa ttatcatctt atatttacca
    13261 ctaatcagtg aaagtgtata actaaatcct gtttcactta aatctgtatt tgatatacag
    13321 tctatactca tatattacac tctccttttg gttagtattg aacaaaaatg tgcgtacttg
    13381 tttttagaat aatttataat aaaattatag tgtaagaata attgaaagtc aattttataa
    13441 taggaggtgt atttactaaa aaaaagttat tttaaatagg attctaagat tagacatttc
    13501 tatataaatt gataatattc atgaaaatcc tattttataa aaaataatat ctcttagaaa
    13561 atctattaaa taaaattcat tggaggaaat ttataatgaa agttttattg ataaacggta
    13621 gcccaaacca atatggatgt acttatacag cactaaatga gattactaaa gtattgagta
    13681 aacacgatat taaaacagaa atactgtatt taggtaaaga aacaatccca ggatgtatta
    13741 gttgcgcatc ctgttttgaa actggaaaat gtattcgaaa tgataaagtt aatgaattga
    13801 ttgaagatct ggataatatt gatggtatta ttattggttc tcctgtttac ttttctagtg
    13861 caactggtca attaacttct tttcttgacc gtctattttt catagctgga agtcgcatgg
    13921 ctacaaaatt aggtgcttca gttgtgtcat gtagacgtgg tggtgcaagt gcaacatttg
    13981 accaactaaa taaatatttt tcaatcagca atatgcctat tgtatcatca cagtattgga
    14041 atcaggtaca tggctttact ccagaggatg ttatgaagga tgaagaagga ttacagacta
    14101 tgcgaactct aggagaaaac atggcatggc ttcttaagtg tatatcagca ggtaaaaaag
    14161 ctggaattaa agaaccacaa tatgaagaaa caattatgac taattttata aaataaataa
    14221 aaaatgcact aatattaatt aaaacaaaac actaatttat aaataaaata agcataaatg
    14281 taggataaaa agcactaaaa cagaggtatt gaattttata tcaaacctct gttttattat
    14341 tacattaata accaaaccat gtataacatc atatatttct aaataaatta taatattata
    14401 ttctaatgtt attgaaatta caataactga atttctgctc ataaatttaa atattctaca
    14461 aatctcatct aaataacaat tttaatagac ctaaaacaat cctcatattg ttcattttgt
    14521 attaaaaata tcacttacaa tcaaaaacca tttattaagt atattcctaa gatatacaat
    14581 atatatacat ggattggata gtataaataa aataaatact tcatactctt ccctttttct
    14641 ccattgtaaa gtaacattaa aggtagagaa gcaatcatca tccattggta attttgtaaa
    14701 atagaatttt ttaaactata atctccattt gacattgttg taaatataaa tatacacatt
    14761 aaaacataac aaatactcaa actgaacttt ttacctctac acaaataaaa aataatccca
    14821 agtataatcc aaattggtcc accttctact aaaaatggta ctggtaccaa aatcataaat
    14881 aaagcaataa ctctgttagt gactttaaaa agtaaagcta ctgtaaatat tcctaaaatt
    14941 ataggaatga ttatcatcaa taatcctata ataaaatatt taatttcttt ctcttttcta
    15001 aatttcttta tgaactctat tgcttgtaaa taaatcgcta ttaaaaataa tgttgaaaaa
    15061 atgttattta taattatagc tccttctgga tgtgaaaata cattattaat tatttgattt
    15121 cctatagcca taataacaga ccaaatatac aatctactta tatatttttt tctgttacta
    15181 gtgtgataaa atccttcaac agccatgaat ataaacagtg gtgctgatat tctgcctacc
    15241 caatgaaacc aaattggtat ttgcattgtt tgtggcataa atgctgctat atggtcaaat
    15301 gtcataaata ttaatgctaa aatcttaata gtaaacccac ttataccttt tttaaaaaaa
    15361 tccatatcat atctcctaat tttgttttat ctctataata atttttgttc ctattccaat
    15421 ttcactactt acataaattt tacctttatg aatctctatt atttctttag aaatagccat
    15481 accaagacct gaacctttat gcatctcacc tgtattagta cctctataat attttttaaa
    15541 aatatgtttc aaatcacttt tgcttattcc aattccatca tcttttatta taatttctat
    15601 attatctttt tttacaattt ctacacttat caaagttcct tcgctattat gaactattga
    15661 attaaatata agattggtta tagcccttct gaataagatt gaatctacat atacttcaat
    15721 taaatcttca ttactttcaa attcaatatt cctattttta tatctattat catttaaaat
    15781 atcaattatt atatctctga ctaaactgac taaatttatt tttttcttgt ccagaactat
    15841 agtattattt tttaatcgag tagataaatt taaatcatca actaaatctt ttatatactt
    15901 agacttatta tatataattt ctgtatactc ttgtatttca tcttcttcaa actcataatc
    15961 tttgtcatta attatctctg catacccttg tatagatgct agaggtgtct ttatatcatg
    16021 tgttatattt gccagccatt cctctctcaa ctcttcattt tcttttctct caacttcatt
    16081 tactcttaaa gtatcagcta acatatttat atttttaaaa acttcttcat atatacctct
    16141 gtccttaaga tataaactat aatttccatt agccatagtc tcaatactcc atagtacgct
    16201 tgaaattggt tttgtaagtt ttctactaaa taaatatcct acacctaaag ctaccacgct
    16261 atcaattact agtgttataa tgataacttt atttaaagtt tttttaatgt tattttgaga
    16321 ataggttaaa attattcttg atatatctct actaggtata cctactatta tgtttagatg
    16381 ttttttgttt aaatattttt ctaatacaaa attaacatac tttgtttcta tatacttgta
    16441 attattcacc atttgaaagg gtgtatattt tttgggagta tcctttggca catttactcc
    16501 ataaacttcc tctcctaaat catttaaaac ctgtatccag gaatttgatt tttctaattt
    16561 ttcagctcca atcttagata gtttaaattc tccatctttt tcatataaat ctttttcaaa
    16621 agaacgtgca aattcttcag gattatttcc agaatctaca actttaaaaa aactattagt
    16681 tgaaatcaca tacaatatag atatgatatt tataattact actgatatag ctacaaacac
    16741 aattgtaaat ataaaatttc tagtaatctt ccacttcata ttctaatctt cctttactgc
    16801 taatttatat ccaagacctt ttattgtcag aatatattca ggctttgaag gattatcctc
    16861 taatttttcc ctaatttttc ttatatgaac cattatagta ttgtcaaatc caacataatc
    16921 ttctccccac actttatccc atagtttttc tttacttata atttgatttt gattatgtgc
    16981 aaataaagat aggattttta gttcctttgc agacagtcct atagattttc catcttttat
    17041 aagttctgcc ctactttcat ttaatatata tgggccaaat gatataattt tttcttcatt
    17101 attatttaga tttttacttt cgttttgagc atcactaaaa taacttaatc ttctcaaatg
    17161 cgctttaact ctaaaagcca cttccttagg gctaaaaggt tttgtaacat aatcatctcc
    17221 tccaatcgct agtccaagta acttgtctaa ttcttcggtt ttagctgata aaaatattat
    17281 tggagaattg gaggtttttc ttatttcttt acaaatatca taaccttcgc catctggtta
    17341 aagtatttta gtacaatttt taattgtata taagagaact catactcact caagtaatag
    17401 aaagagatat atataattca aattatatat atctctcttt ttattactca ttaactataa
    17461 atcaaatttc ttacaactac attacaaaat tcattatata ttgaataacg ctctttactc
    17521 cagcgttcta aattcaaaat tctattttgg gttaattctt taataagctt tatcccattt
    17581 atgtattctt cttcttccac ttcgatatct cctacatttt cttttgcacg tttaattatt
    17641 ttttctttgt ctttttctat gttaagtaaa cttctttctg aatcatttat ttctttgtct
    17701 acagaagaag gatttccaga cgataaaaaa aagttgagaa ttattgaagt tattgaattt
    17761 acaactacat gctctgaggt aatatctgaa actttaataa attttttcaa taattcatta
    17821 tacatgtcat aatagttaga aaacacactt ggtcttgttt ccaaatactc tcctaaataa
    17881 agtatagata aaagatttac aatttcattt tttgtatttt tatccaattt attaaatggt
    17941 tctaaaaatg aacttaattt gtttaaatat ggaaatttta tatattctat atgatatgga
    18001 ttcatataat ctaaaatatc atctttatac atgatattat atttccttaa caggtaatcc
    18061 atatttttag tcaaaacttc aagtttaggt cgttcccaaa tagaaatctt aaatttaggt
    18121 ctattttttt ctataaagtt attaagcata tcttttgcag gatttgataa gaaattggag
    18181 actattataa cacagcgatt aatatccata ctcatagctg atgtaagtgc acctagtaac
    18241 ttatctgctg gaacaccttt tatgtaatgt ttacattcta caaaccattt ttcaatatta
    18301 gtttctccaa gaagataatc cattttgata taagtacact ctatatctct tcctccatca
    18361 gctgggctag aagattttcc tgttcctttt ctccaattta aatcctcaca ctttaatcct
    18421 ttaagcaaat caaaacaaaa attttcaaat tctgtatctg ttagattgtc aaaattaatt
    18481 ttataagaca taattcatgc tcctttattt tagataatat tgaaatcaga ttattctata
    18541 ttagttatat ttacttgagt tttaataata attaatgtga aaaatatact agaatgtttt
    18601 ttaaaagttc aatatagaaa tatacaattt tacttaagcc tatacttgag cctataatta
    18661 atactgctat agcaatagtt gaaaatagta taactaccaa ttttttaatc tctctatttt
    18721 tagaggaaaa tctaatataa cttttgattt ttggaataaa attatctctt agccaagtta
    18781 tttcatacgg tatcctaaat ttacctatat aataataatt ataataattc aattcattgt
    18841 aatatgttct cttatttcta tataaagtat atttgtcaaa accaacattt atatctggag
    18901 tctttatata ttcaatatta agttcaactt cattccaaaa gattctttta taatattctt
    18961 ttcccaattt attattaata caattaatta cttctattct atttcttcct atattaaaat
    19021 cattaaatct gactgtatta actctacaat tattattaaa aaaatttata tttcctttta
    19081 tctcagtaat atcattgtca ctaatatttt caatgtttat tatttgttca ttcattaaat
    19141 tatctaaact tacatttact cttatatatg gagtctcatt aaatgtatag ttacacatgt
    19201 tctctggttt tattattttt ttaaattcat tatcactaat cttaaaagta taatgtacaa
    19261 taagcaaaag cacaagactt attgtaatat atatgtaatt actattaaca aaattgagtg
    19321 tttgtgataa gttcaagatt aatggaatta tcataaatat aatataattt ttatattttt
    19381 tattacttat tttattagat ttttcctcaa atgaattcat aacaaaatta atcattatat
    19441 cgtctaactc ttctctcgtt aaaggagaac tacttgttcc taataataat atcttaccta
    19501 agcatgaatc tattgctaaa tctatctttt tagggtcaag tattatatct ttttctgata
    19561 aatatttatt taatttatct ccaatatatc ccaaaatata ttttttattg aatggtataa
    19621 catctattga taccttgtga ttttctagta tagaagtaaa ttcattcagt gattttttac
    19681 tttcttcatc aattattcta ttattatatt tatctactga cttaataaat atatttgcat
    19741 tattcctaca ttgaccaata aggtataatt tatattcttt acttgaagat tcatcatcta
    19801 taagctctct aatccatttt ttaatatctt ctttgttaaa taaattgata gatgatttta
    19861 cttgtataac ttttattagt gttgaatctt taaataaagc aatatccact ttatcttgct
    19921 ctgttggaaa ttctaaagct atatgagtcc aatttttgtc ttctaatgac tctattatta
    19981 aagctaaacc ttgataaaca taacctctta acgcactttg cccagacata aattttacct
    20041 ttctttttat attatctaaa ttaataataa cactttaata aataaattgg tatattatag
    20101 aattcaaaaa aataaggtga tagttttttt actattacct tattttttat gtttatagat
    20161 tatctatata ttcgtttctc aatttatttt atcttattta ataatatatt atcgaattta
    20221 tttctttatc taaatccaaa gattcaattt taaaagttct ataatgttta atttgcactt
    20281 cttccattaa tttaagataa ttgttattat tatctacgaa atcagataat attctactta
    20341 atttatccga aaaatccata ttatattttt tcttaaataa tatagctctt agtaaattaa
    20401 gttctatata tggattttct tccaatacaa catctaaact tttattttcc atggcatttt
    20461 catacttttt attataataa tcttgtgtat ttaagttgta tactatagaa tcaatattta
    20521 aaaacactgt atcctgtaat ttcttttcat aatctccaaa atttatttga accttatttt
    20581 ctttaaatgc ccaagtgttt tctatttccc aaatatcaaa ttctatttga tttactcgaa
    20641 ttttatagcc accaaatcta ttttttttat attgaaattt atacaaaatt tcatctaaat
    20701 ttacatattc attatttttc tttattacta tatcaaaatc tcttggcaaa tgattaaatt
    20761 tattatccat atattctcgt acagcaccac caaataataa taactctcca tacagtttta
    20821 aagtttttat taattcagat gcttcattat attgtcttaa taatttttca aagtcacatc
    20881 ctaaattttt tatattcatg gtagcacctc ctaataattt tatggtaatt taactcccat
    20941 taactcttta tatagattca ctgcatatga atcagtcata cctgatataa aatcggttat
    21001 aagtaataat ttattatact tttttacctc tttaaagtca atctgtctca ttaactcatc
    21061 tttttttcta ttaaagttct tttcttcatc taggcaacat atatatttaa aattatcaga
    21121 aattaaataa tataattttc cttctttttc tttatgattt atttctccaa catttcctac
    21181 taatgaatca acaaatgtat ctaataatcc atttattact ctatcaccca ctagctctaa
    21241 agttaaaact tctttattaa caaaacaata ttcttttgca agacgtttaa cctcttttat
    21301 caattcactt actttacttt tcttaagcaa gttttctata ccatattttc caagcattat
    21361 atcttcataa ttttctttaa acatatttac tacttgattg aacatcattc cttgagctaa
    21421 aactttaaaa ttttgaatat tagctaaatt agattctggt atttcattct tattatttct
    21481 ttctatttgt tcatctaagc ttttaaacat tttttcatat aatattttac tttcttcttc
    21541 tttttcattt tcttttaaag ccttttttat ctttttatat tctttatccc aaggaagtat
    21601 accttttttt acagcatctt ctacatctgc taataaatat gcaatatcat ctgatgcctc
    21661 aagtaaatac gttacaggat gtctaatacc ttccttcaat cccactttat caaaaacatc
    21721 ttcaaccagt ttttcctcag ccgaaaaata tccaaacttc gattctgctt tccctttttc
    21781 tgagtttgat ttccatggat atttcattat agttgctaat gtagcatatg taaaattaac
    21841 accatattca tcatttaata attgtaattt agatacaatc cttaaagttt gagcattacc
    21901 ttcaaagttc tcaaaatcag ctctttgttg ctcatttaga ccatcaccat ttttttctat
    21961 ttttaaccat tctttaaacc aattccttat tacttcttct ccatgatgcc caaagggagg
    22021 attccctaaa tcatgtatta aagcacttac ttgtaaaaga gctgataact ctctaacctg
    22081 tttataattt tcaaattcac cttcacttaa taaccattct cctatagcat atccaaatga
    22141 cctcgctaac gctgatgctt ctaaagaatg tgttaatcta gttctagtaa aatcattttc
    22201 ttgtaatgga aatacctgtg ctttatcttg taatcttctt actgatgatg agtatactat
    22261 tctatcatag tctttgtcaa attcatttct atgattatac ccctcatcat ttatgtttaa
    22321 gttttgatta attggtgtag acgatgccct aacacgttca ccacataaaa gttttttcca
    22381 atctaatctt tgttccataa aatatccccc tagtttatag ttattaatta aattatactt
    22441 ataaaggaaa tattttacaa ctttcgtgca aatttttaat acttataaag atattaaaat
    22501 attgtatagt tttacatttc tttgttccaa tatagaattc tgttttttca ataattaata
    22561 ccaattttat tttattaaat cgagatattc ttcaaattat aattaatata tgatacttat
    22621 tatatattaa tttaatgtca aaatagtaca taataagtag ataaatttta tttaaaatgt
    22681 aaagaaagtg taaatttata atctatttat ctgtagaatc caaagtttta taggaagtac
    22741 taaatctttc attgccattt atcataagta tattattttt cttaatttat agaacctatc
    22801 ctattatatt ctccactttt gcaactaaca tgattaatct tttatctttc tataaataac
    22861 atagctttct taattgtttt ccatacatca ccagcatata tttgagtaaa cttttctcct
    22921 gtagttttag ttatctccac cattttttac tagctccacc acctacaaca tatagatttt
    22981 gtgttttgtg tggcttatag tcttttatat ctgtaactag ataactttca tttgctcttt
    23041 tataatataa gcctaacagg tcagctgcta ctttatcatc tccagagtag actatagtat
    23101 gtttatatct attataatta tcttcttccg ctgaactaac atgtttagtt attgcttcta
    23161 ctattgcagt agctaactta tcagctccta atactaaata tttattcgca tcttcactat
    23221 ctacaaagca tacttctatt agcatacttt tagcttttgt tttctttact acatataatc
    23281 cacttccatc ttttacacct ctatttgtga atcccagctt agatatattt ttacaaacgt
    23341 ctacagcatc ttgatactgc ttacctttat atgtgtaaac ttcacatcct cgccctcttc
    23401 ctgcattgaa gtgtattgat atgaaccaat ctaagtcttg tctattcgct tgtgcaacta
    23461 ttttagatag acactcactt tgtgtagatg ccttatctat aatagtacaa tcaactacat
    23521 tattgcctag tttttttagt ttatctatta ctttgtatcc tacatttctt gtctctaaag
    23581 actcgcttat tattcctact gctccactcc ctgctcctgt ctttgtatgt ccacaattta
    23641 tccctatttt catcttattt attccccttt ctaagttgtt ctagtgcttc ttttattcta
    23701 gctggtactg gtaaacctat tgctgcacaa ttttctgtta tacttaatgc ttcgtttgct
    23761 atataaaaat aacaaactaa tgttttaaat atccaatctc cattatttat taatctgtct
    23821 aataatgctg ctactgcaag aattaccagt attccacttt tttttgcaat gcctttaaat
    23881 cctacattac tagaaagttt tttaagtaca aaccctttaa ttattccaaa tatatagtct
    23941 acataagtgt taacagaata gctagtacct cctcccaagc tccaaatagc catgtaaaat
    24001 acgtccctat tagagcagta attgtattta attttccatc atatatactc atattctctc
    24061 cttattcatt tataaataaa aaaaacaaac atagttttat ttgtttgctc ttttataaca
    24121 tttttctatt ttaaattttg atattcttac aattggttca tttttattca ttatagaata
    24181 ccacatccaa gctctttatt tacagttaat aataattgat tttctaactc ttgtattctt
    24241 tttcttcttc tgttaattct atttttagtc tttttataac tggtacatta ttaattgtat
    24301 ctatcttttc tatataacaa ttatctggta ttgagttaaa aggaatatca atataatcta
    24361 tttttgatat tccttttctt ggtataacat ctccttccgt ttctccatat atcgcaatta
    24421 tttctccgtc ttggtcaaag attattcttc ttcctatttc catttttatt ctccttttta
    24481 tttatatcct agtgcaaacc aactaattgt agcacttgag tcaaaatctt cgttaggtat
    24541 tggtaaccta aatccaccac taaaatgtcc aactgaagac ccattatcaa catccatttc
    24601 tcgatttttt acatgataaa tagaatatct agtgctagga attgattggc tatttgtaca
    24661 aaaaattagg ttagatttta attccatact agaatcgaaa gaaactattg tatatttatt
    24721 acgtgaattt ccagcttgcg caaaaactaa attaggctta aatgctaaag aatctacagt
    24781 aaaagcaccc catacccaaa cctgttcaga attataattt tctatatatg ccttttgtaa
    24841 acgaagcaaa ctgtttccgc tttttgctgt ttttattata ttttttatgc ttgacacttt
    24901 atctattaaa ctttgcatac tctcacttcc agcagtagaa acatttttat ttgtcaaatt
    24961 tgttgcaaaa gtatttttta acgtttctat ttttgttttt gttacatcta gtttatctgt
    25021 tccagcaaaa ggactaccta atgcacttgc tatattattt ttactaattt gtaattcatt
    25081 tttcacatcc ccaaaagttg ttataagttc acttaaactg gcattttcat ttaacttttc
    25141 taacataaaa tatcaccttt ctac
//
LOCUS       DAEORZ010000047        24723 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEORZ010000047 DAEORZ010000000
VERSION     DAEORZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.047"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            506..1198
                     /locus_tag="KRH39_001753"
     CDS             506..1198
                     /locus_tag="KRH39_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8511663.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            1394..3454
                     /locus_tag="KRH39_001754"
     CDS             1394..3454
                     /locus_tag="KRH39_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8511664.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3660..3983)
                     /locus_tag="KRH39_001755"
     CDS             complement(3660..3983)
                     /locus_tag="KRH39_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBG8511665.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(3976..4677)
                     /locus_tag="KRH39_001756"
     CDS             complement(3976..4677)
                     /locus_tag="KRH39_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBG8511666.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(4955..6340)
                     /locus_tag="KRH39_001757"
     CDS             complement(4955..6340)
                     /locus_tag="KRH39_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG8511667.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIGFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(6448..7686)
                     /locus_tag="KRH39_001758"
     CDS             complement(6448..7686)
                     /locus_tag="KRH39_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG8511668.1"
                     /translation="MELFIEVIKFNIYISPLILFFILFTKKISKYTFKFNYFICLLIV
                     VRMMFISKINLNILNINNTTINIYKHNIDNYIPYDQGITLDIFMYIWILGIIAISFYY
                     LRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTR
                     CKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIYWFNPILYLLKKYFH
                     ELCELSCDEIVIDKCTTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKKNIILKKRL
                     NSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGT
                     HIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFK
                     KTIPLYDKEGIKIIGRFEIN"
     gene            complement(7699..8067)
                     /locus_tag="KRH39_001759"
     CDS             complement(7699..8067)
                     /locus_tag="KRH39_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8511669.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
     gene            complement(8595..9017)
                     /locus_tag="KRH39_001760"
     CDS             complement(8595..9017)
                     /locus_tag="KRH39_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBG8511670.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            9495..12398
                     /locus_tag="KRH39_001761"
     CDS             9495..12398
                     /locus_tag="KRH39_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8511671.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEAEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            12431..12877
                     /locus_tag="KRH39_001762"
     CDS             12431..12877
                     /locus_tag="KRH39_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8511672.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            13000..13302
                     /locus_tag="KRH39_001763"
     CDS             13000..13302
                     /locus_tag="KRH39_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8511673.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            13483..14889
                     /locus_tag="KRH39_001764"
     CDS             13483..14889
                     /locus_tag="KRH39_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG8511674.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPDNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            15233..16291
                     /locus_tag="KRH39_001765"
     CDS             15233..16291
                     /locus_tag="KRH39_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG8511675.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMEKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            16390..17076
                     /gene="araD"
                     /locus_tag="KRH39_001766"
     CDS             16390..17076
                     /gene="araD"
                     /locus_tag="KRH39_001766"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBG8511676.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            17094..17741
                     /locus_tag="KRH39_001767"
     CDS             17094..17741
                     /locus_tag="KRH39_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG8511677.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(17914..19281)
                     /locus_tag="KRH39_001768"
     CDS             complement(17914..19281)
                     /locus_tag="KRH39_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBG8511678.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(19471..19559)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            19987..22107
                     /locus_tag="KRH39_001769"
     CDS             19987..22107
                     /locus_tag="KRH39_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBG8511679.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLKSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            22211..23464
                     /locus_tag="KRH39_001770"
     CDS             22211..23464
                     /locus_tag="KRH39_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBG8511680.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKNYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(23618..24679)
                     /gene="ytvI"
                     /locus_tag="KRH39_001771"
     CDS             complement(23618..24679)
                     /gene="ytvI"
                     /locus_tag="KRH39_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG8511681.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
BASE COUNT         9664 a         2811 c         3896 g         8352 t
ORIGIN      
        1 gtttgtattt tagttatata aattttaata gaatatgtac agtatatagt agatagaatg
       61 tataaaaata gaatttaaat aagtagctat tattgcagtg aaatgaatgt ttctaagtat
      121 atttgggtgg aagaatggtg gaagaaaatt agtgaaatgt aattcttcca ccattcttcc
      181 acccaaaaaa taaaaactgt tcaataagaa ttaatataca tcaattcagc tgaatgcaat
      241 caaaatactt aaatgaaagt aatctgaaaa caacataatt caaatcaatt tattcaaata
      301 aacctcaatt tatcttaaat cttaagaaat atttaagaat taggataata ttttcttaag
      361 atttatattt tattatatag ttataaggga aagttacaaa aatgtaagcc aaagtaatat
      421 aacaatttag tcattgttga gaaagggagt gaaagatatt gtataatata gataagtatt
      481 attatacaat agaggaagtg aaaatatgta caatatatta gtagtagacg atgataaaga
      541 aattgttgat tcgatagaaa tatatcttaa atcagaagga tttaaaatat ataaagcata
      601 tgatggaatg aaagctttgg aaataataac agagaatgac attcatctaa tactaatgga
      661 tataatgatg cctaaattag atggaatcaa agcaactgta aaaatacgag aagaaagaaa
      721 tattccaata attttaattt cagcaaaaag tgaagataca gataaaataa taggattaaa
      781 tattggagct gatgattata taacaaagcc atttaatcta ttagagctta tagctagggt
      841 taaatcaaac ttaagacgat atgtaacttt aggtaattat gaagatacat gtaaagaaat
      901 tctaaaaagt ggagctttgg agttaaatac actaacaaaa gaagttaaaa ttgatggtga
      961 aaacataaaa atgactccaa ttgagtataa aataattgaa cttcttttag aaaataaagg
     1021 acgagttttc tcaatagatg agatatacga aaaagtatgg aatgaggaaa gttttaattc
     1081 agaaaataca gtagcagtac atatcagacg aataagagaa aagattgaaa taaatccaaa
     1141 agaaccaaga tttttaaagg tggtgtgggg agttggttat aagattgaaa aattttagtg
     1201 gaaaaattat aaaagaaaag agtttaatat ttaattgcat tttgatttta gtcattttta
     1261 catttacaca aatgttagga attttaatat gttttaattt tttctctgag agtttaattt
     1321 actctatggg gattatgctt ttcttaaact ttattttata taaaatactg acaatgtaga
     1381 tgaggtgggc aacttggaga aatatagagt attaaagtta gtttcattaa tcataatatc
     1441 tatctcattt ccattaacta tattgttggt gacaatagga cttgctgagc ctaaatacaa
     1501 tatacaaaaa acttctacaa agagtgggat ttttgatata tctgatgatt tttatgatag
     1561 taatatattt gacgaaggaa caatggcaag tctattaccc aagtttaagc tatctgtagg
     1621 tggtaaatta actagtaaag aaaaaggtaa attccaacaa gaaggtaaaa gtatagattt
     1681 aagtagtagt gattctgagt atgataaaat catgattaat gaacaagctt ctgaagaata
     1741 tttaagttat ctaaaaaata taaaagtgta tgttattaat acatacacag gagtatttta
     1801 tacaaatacg tcatatgcta gtttatatga ttttaaacaa aaaactaaga gctattgtaa
     1861 tgtagagata ataaataaga taggaaagag ctcatatatt aaaacaataa atggagaaaa
     1921 atttgaactg aataatctga gtctggaact aaatgaagta gatgaaggtt ttgaggcata
     1981 tgtatctttt ccagaagagc caactataga agatggaata gtatatacta attttcaaat
     2041 atttaaacaa gctacagaaa aagtaagatt attttttgta gctagtttaa gtttagcaat
     2101 tatatgttta ttatctataa ttttatatat caaaataaaa ggtgaagttt taaaaagaaa
     2161 taatgtattt ttactaatat acagtaagat acctatggaa tttaatgtaa tattagtatc
     2221 tatttcaatt gcaattttaa ctatatatgc tcttgctgta tcttatcata ttgatgtaat
     2281 tgcattagtt tctttatctt tactgatagt atcaaataat atatttttta taaatgtgca
     2341 attgagttat ttagaaaata aaatgtgtat atttaagaca agtattatag taagagtgat
     2401 tttatggtta ataaaaactg taagggaggt cagtaacgct ataaagaaag tatctttagc
     2461 aaggaaagtt attgttatag tgataatatc tttagcttta atcatggtaa ttagcactcg
     2521 aagtataggt atgggattac tgttttctat atgtattttt ttaggattta caatatatat
     2581 aactaaaaga ctttcatatc ttagttacgt aatagaaggt acagaaagaa taaaaaaggg
     2641 agaattagat tataagataa aaattactgg gaatgataat tttacgtctt tagctgaaaa
     2701 tataaataat attggagaag gtcttgatag agctatagaa gagcaagtaa aaagtgaaag
     2761 aatgaagtct gaccttataa caaatgtatc tcatgatttg aaaactccac ttacttctat
     2821 aataaattat gtagatttaa ttaaaaaaga agaatcaata caaccagagt atataaatga
     2881 ttatataaat gtacttgagt caaagtcaaa aagactaaaa ttattgatag aagatttatt
     2941 tgaagcgagt agagtaagta gtggcaatat agaattacaa attagcaaga ttgatttagt
     3001 gcaactatta agacaaagta taggtgaact tgaagaaaaa ttatctaaaa ataatttata
     3061 tttaaaatta aatgttccaa atgataaggt gtatatatgg gcagatggaa gaagaatgta
     3121 tagagtattt gaaaatcttt tatctaatat agcaaaatat tccttagaag aaactagagt
     3181 ctatattgat gtatatgact atggtgaaaa tgtaaaggta actatgaaaa atatatcttc
     3241 ttatgaatta aattttgacc caagtgagat tatggaaaga tttaaaagag ctgatgaatc
     3301 aaggaatact gaaggaagtg gattagggct tgctattgca agagatttag tagctttaca
     3361 aggtggaaaa ttttatatag aaatagatgg agatttattt aaagcaaatt tagagtttca
     3421 aaaatatgaa gaagaaataa caaatatgga atgagatagt atttaagtat atatattttt
     3481 tttactgtcg aaagctttta ttataatata ttagaatatt tacagtagag aatgaagagt
     3541 aaaaacagag taactttaag tcactctgtt ttttatttaa aatcaataat ataacaactt
     3601 aaattaaaat tgtacacata gttttaaaat aacttataca ataaaacatt atccaaatat
     3661 cacaagataa ttccaaataa atatatagat atcactcctg taataataga aataccaata
     3721 gatttaaact tatatgcaac aagaagtaca atcataaaag atataatttt agggttagct
     3781 atagataaat atagactatc tccattaaaa aatacatctt tagcagttaa ggaagtaagt
     3841 attgctacag gtataaattt catgattttt ttcaaaaaat caggcatctc tttttttgaa
     3901 aaaattaaca taggaagcac tcttggtaga tatgtaacaa tgtacattcc aattataata
     3961 aaaaatatat aattattatt cataagattc ctccttttta cagtaagatt cttctataaa
     4021 gaaaccaatt aaagatgcta aaattgatgc aactattatt actaaattgc ttttgaataa
     4081 tgcatataaa ataacagaaa taatgattga tgatataaat actacaatat aaatcttatt
     4141 ttttatttga gaaactatta aagctataaa catagaagtc aatatgaagc cagaaataga
     4201 actgtctata gatatacttt ctcctaaaaa tgctccaata aaattagcta aaactgatga
     4261 aattaagcag aagaaattaa gataaatagc atttctatca gaccaatttc cagcagtaaa
     4321 tttttcataa ttaactgcaa aagtttcatc tgttatagta tgacaaaatg gcaatataaa
     4381 ttttggactt ttatttttta tgtacatgtt caaattagaa ctcattagaa aatgtcttaa
     4441 gttaactata aatgtagtca aaacaataga tgttacactt gcaccagtag aaatcattga
     4501 agctgcaatg aattgagaac ttcctccaaa tactaaagtt accattagtg ctatttgaaa
     4561 aggtgaaaga ccagattttt gtaatagtat tcctcctact atacctatag gtatataacc
     4621 taagaatata ggaattgaag caataaagct ttcttttaat tgttgagttc tttccacaat
     4681 aaatcactcc ttttgtatta aatgtattaa agtattcgta aaacagaaaa atagattaat
     4741 tgtataattg caattataat taaaaataaa tttggattaa tatataaaat actatacaac
     4801 aaatagataa tcttaaacgg ttattaaatt ttatctattt gttattatga tgtaataata
     4861 catattaatc tctataagtt aattactatc tggtgtaatt ttattttaac aacaaaaaaa
     4921 aatacacaca attaaaatat tgtgtgcaat acaattattt tttctcttct aatagtcttt
     4981 ttaatacgct caaaccagct ttaagttctt ctgtactttc tgtagcacat acagctattc
     5041 ttattgcaga aattggtttt tcttttccaa cagcaaaacg ttcagaagca tagacctgta
     5101 caccttcttt aagtgcttgt atctcaaatt tttctgccat tattccatca ggtagaatca
     5161 accatctaaa tatacattct tcatttccca aaagattata tccagataaa tattgattaa
     5221 taatcttgtt tcttcttctt gcagatttac ggcgtgcatt aattaattta tcggcttcac
     5281 ctgatgtaat catgcgataa gcaatttctg ttaaaaaata agattgagat agattaatgt
     5341 tgtataatgc gcttgataat gcctctcgat actgtttagg tgtagagata tatgctagcc
     5401 gtaaactagg tgcaataatt tttgataaac tcgtaatata aattgtttga ttgggcagat
     5461 aggaagccac tggatttaaa tgtgtttcat taagtaaact atgaatagca tcctcaataa
     5521 caattaaatt gtatttagat gcaatatttg caatcatttt tcgtccattt tgtgacatta
     5581 tatgtgtagt tggattttga taatctggta ttatgtatag acctttaata ttttcatttt
     5641 tacaagcata taatagtcct tcttcactaa tctcattatc ttcttgttta attggaatta
     5701 gttgaatccc aagcatttta gctgctgtct ttattccagg ataagttaat gggtctacac
     5761 ctattctatc tccatgttga aacaaaccag ctaaaatagc aacaattgca ttttgtccac
     5821 cacttgcagg aagtaagcta tctgatgtag tttcaagccc agctttataa attaatttgg
     5881 caatagcttc tttttgccat ttagcaccat ctctttgtcc atattggaat atggttttaa
     5941 aacctatttc cttaattatg tgtttaaaca aagtattcat ttcttctagc gtgaaatctg
     6001 gattcattgt gcccatttca ataaatgtaa ttttattatt actggacatc aagaatagat
     6061 ttgaacgtgt atcataagat acaaaagttc cacttcctgt aactccactt attagacctt
     6121 ttttttcaca aattttaaaa gctctagaga tagtactcac attaacatct aagaaatctg
     6181 ccaattcacg ttggggtggt aatttagtac ctggtaataa aaatccattg ttgatatctt
     6241 gttctagttg ttctgcaagc gccttatata atatttgccc ttttgaaggt ctctttggtt
     6301 tccagttcat aggataattt tcaaaagaat taattggcat aagttttcac ctcacattat
     6361 taatttgatt ttttctttat ctaaaacaat aaattctaaa gatacaaata aaaactgata
     6421 ggaaataatt ctatcagttt ttgtctttta attgatttca aatcttccta taatttttat
     6481 accttcttta tcataaagag ggattgtttt tttaaatata ttaaaatttc tttttcttgt
     6541 atataaaaca gcttcttctg gtgtctttgg ttgattatca caatcgtaca aatctctttt
     6601 ttttacgtag ccgacttttt tacctctacc tattgtttgt ataagttctg gttcaatgga
     6661 atttccattt gagtctatta tatgtgtacc ataggtttct ccatttttat tataattaat
     6721 cttaccaata gtatattcta catcttcatt ttgtttaaaa ttggagttga tagaaaatat
     6781 tgaccctatt attataaagg ttaataaaaa cttaagacct aaattaattt tctttttatc
     6841 ttgattaaac atagaattta acctcttttt taaaataata ttttttttat ttagaaactg
     6901 agatacgaat gatgaatact tcaaagattt ttgatacttt attgtatcta atattatata
     6961 tgcatactct ttaatttcac tcgtggtaca cttgtcaatc acaatttcat cacaagataa
     7021 ttcacataat tcatgaaaat atttttttaa taagtataaa attggattga accaatatat
     7081 gatacaaatt atactaatta gaagcttaaa taagttatct tttcttttat aatgaattaa
     7141 ttcatgttta aagatgaatc ttaactcttt attattgtac tttttttctg gaattattat
     7201 tttacagcgt gttaaaccta taatggcagg agaataaata cctttaacct tatatatctg
     7261 aagtttttta tttatactta gttcttttgc ttgttcatct aagatactag taatatctat
     7321 ttctaaaatt tcttttttaa aattttctat gtaattataa aatttaaaat ttgttcttaa
     7381 atagtaaaaa gagattgcta taattcctaa tatccagatg tacataaata tatctaaagt
     7441 aatgccctgg tcatatggta tataattatc gatattatgc ttatagatat taatagtagt
     7501 attattgata tttaatatat ttaaatttat tttagatata aacatcattc tgactactat
     7561 taaaagacat ataaaataat taaacttaaa agtatatttg cttatctttt tagtaaataa
     7621 aatgaaaaaa agaattagtg gacttatgta aatattaaat ttaataactt ctataaataa
     7681 ttccataaaa tacgtctcct attttctttt ttttagccat tgttcaagtt cttttatgtc
     7741 atcatctgat atttcatttc cacaacctaa tgatgatata aagctatgaa tagaattttt
     7801 atgtataagc tctaaaaaat cctttgtttc aaattttaga tattcctcat gtttaacttt
     7861 aatagtgtat aaagtatttc taccatcttt ttcaaattct aaaaaacctt tatcacttaa
     7921 tctagaaagt actttaccag tagtaccttt tgtccatcca tgagaaattt ccatataatt
     7981 agctacttct ctggctggta ttttatctat gcctttatcc catatgaaat tcataactaa
     8041 caactcggat tttggtattt ttttcaatat aatcactcac ctttaacttg attatattag
     8101 acaaatgtct aatagtcaat ataatatatc atcatgtata aataatcttt atattatttt
     8161 atgcttgaga actaagtagt atagatttat agataaaaaa gatattgaga gttttatgga
     8221 agaggggaat atattttcta ttagagataa gaatatgaca tatatttttt attgatttgt
     8281 acaaaaatac tatatagtga ataaattaat atcttttcta aattagaaga ggagaatctt
     8341 attagataaa gattatcctc tttattgaaa gtttaaggga tattagagat tattaatttt
     8401 tctctctttt tttaactaaa agcaataata cataaaatta ttattgctaa tgatactctt
     8461 aatgtggtat atagtcatag tgtcacatgt ttagcaatag ttttttcagt aattattgta
     8521 acgaatagta ggactcataa aatacttaga aatatgtttt tagataaatt ttaaatatat
     8581 aatctaacct atatctataa attaacacta ttattctcca attccatttc tagttttttt
     8641 ataaatttat taaattcttc tggttgcctt tttccaaaca atactcttac aagagctgaa
     8701 ttgatttttc caaatatatt cctcttgctc tcactttcaa taaaaataag ttttgtattt
     8761 tctaagtcaa tatgtactaa ttcatatcta gtaatagtac tttctctttt attgcttgtt
     8821 tttatctcat atactctatt tttttcgaaa ttagttattt caaccttaaa tcttttatta
     8881 tttttttgct gtacaaatgt tcctactgct tttttctcat taaaattagg aaaatttagt
     8941 cttgcagatt ttgtaaatat attaaatatg tcttttgcag aatattttaa taaaacttca
     9001 gttttaaatg tattcatatc gttttccctt ctgtatataa atttaatatt tataagttaa
     9061 tttttaagtt aaaatagtat aatatgccat attaaatcat gttaaaacta tttatatagc
     9121 taaaatctta cctatataag tatgtatact atcatataaa aataaaatct taaaatagaa
     9181 agggttaaaa acacagttta ttataatttt tattgttgag tagatacgta ctattatata
     9241 tgattgagca agtttttcaa tcataaattt ataaagtatt ttgcattaaa atttaaacaa
     9301 agagttaaat tatttaagat tatgaataac taatacattt agtaaattac taaatataca
     9361 aaatgtaaat acttatcaaa atgtataaat acttatgaca tctagttact tacagggttt
     9421 aagtagaaaa aaatatcaaa gtaagtatgg catgatagtt gcttatatat ttatcaagaa
     9481 aggagttatt aagcatggag ttgagagata aacttgtaaa catcataaat acagaaaata
     9541 aaaaagatcc attaacagat gttcaaatag caaagttttt aagtacaact agagagaaca
     9601 taactaattt gagaaaagaa cttaatataa gtaattcaag gcaaagaaga tatccttatt
     9661 taaaatcagc aatttcggca atattacaaa agaataagaa tatttctata tcagaaatta
     9721 caagggaatt gatgacagaa ggctttaata tatcgagaag agttgtggag gaacttttac
     9781 ctaaagaaag tttagaaatg gatgtagtag aggaagctga agaagaatcg aatgacccat
     9841 ttgaaactct gataggtaat aaagggagtc ttagaaatgc tgtagagcag gctaaatctg
     9901 ctatattgta tccaccaaaa gggttaccaa cgttgattat tggggaaagt ggagttggta
     9961 agagtttatt ttctagacat atgtatgagt ttgcaaagca aaaaaagata gtaaaagaga
    10021 gctccaattt tgttgtattt aactgtgctg attatagtga caatccacaa ttattattat
    10081 ctttgttatt tggatataaa aaaggtgcat ttacaggtgc tgaatatgat acaccaggtc
    10141 ttgtagaaga agctgatgaa ggagttttat ttttggatga aatacataga ttgcctccaa
    10201 aaggtcaaga aatactattt tctatcttag ataggggaaa gtttaggaga ttaggtgaaa
    10261 ctaatgctga aagaaaagta agtattatgt tgattggggc aactactgaa aatgtagaga
    10321 ctaatttatt attaacattc agaagaagga taccaatgct aataactttg ccaccattac
    10381 atgatagatt actaaaagag aaaatagacc ttatatacaa tatctttcaa caagagtgta
    10441 atagaataaa tgcaaaaata tttgtagata aaaatgtgat tgaaatactt gcactaaaaa
    10501 agtttagtgg aaatatagga caattacaaa atatgattca ggttctttgt gcaagagctt
    10561 ttatgaagtt tataaattcc aaagatgaag atagtgaatc tatagtagta gtagatataa
    10621 atgaagtttt aaagcttaaa gattctttta aagatgctgc ctttcaagag attgaatata
    10681 cagagataag aaaatatttg aaaaatgcta tatttatacc atttaattta gaagaatcat
    10741 cagtaaaagg aagtctttta agtgatgaat atatattacc agaggacata tacaaaacaa
    10801 ttgaaaagaa atattatgac ctaaaatccc tagacattga agatatagat atagagaata
    10861 ttttatggac ttttatttta aatagatttg ctgacttaaa gtttaatctt gatagtaatg
    10921 aagaaatttt ttcaatgagt gatttaaata gttttgtaag tgatagttta gttaaactgg
    10981 taaaagaact tatgaatgat atagttgaaa aaaatatgaa tttagaagta aataaaaata
    11041 tgttcaaata tctagcaata cacttagaag aagctataaa aagaattaag ctaaatcaaa
    11101 agataataaa tgtaaattta gaaaaaataa agattgactt ttcaaaagaa tatgaaatta
    11161 gtaaaagttt tgcaataaaa attgaggaat caattggtat taagatttca gatgatgaaa
    11221 ttggatttat aacgctctac ataaagtctg ctctaaaaaa tgaagtgaaa aaagataaag
    11281 tagggttaat aataatttct catggacgta tagctacaga gactgtaaat gtagttaaag
    11341 aacttttagg tgtaaagttt cctgtggcta tagatatgcc tttagatgaa aaaccaataa
    11401 atatatacaa taaggcagtg gaattgtcaa aaataataga ccaagggaaa ggcatattgt
    11461 ttttggtgga tattggttca cttacaaaca taggtcaaat agtaaataag agaaccaata
    11521 taagtactaa aactattgat agagtagacc tcctaatggc actagaagca actagaaaag
    11581 tttctatggg agaggaagaa cttgatgaaa tatttttttc tttgctaaaa gataggatgg
    11641 gatataacta tgaattagaa aaacacatag ataaatcaaa tgcaattata acattatgtc
    11701 ttactggaga aggaactgct aagtatataa gtaaaacgtt ggaagaaaag tatgataaca
    11761 ctaaatgtta tcaaatgagt gcattggatg aaaatttatt ttgtaaaatt gaaaatctaa
    11821 aagagacaaa caatatatta gctattgttg ggacaataaa ccctaagata ccaggtataa
    11881 attttatacc ctataataaa gaacttttca aaaatttgga tatctatcta tcaaaatcaa
    11941 aacaacaaga tgacagacca attaaatcgt atgaaagaat gcttgatgag gatttagtga
    12001 tttttgaacc agatatatat tttaaaaagg atttattaga atatgtatgt tcaattctta
    12061 taaataaaga atatgtagaa aaagattact tagattctgt tttacataga gaagaaatgc
    12121 ttccaacata ttctaaagga gctataggtg tgcctcatgg agattcttct actgtaaata
    12181 gtacaaggtt tgtttttgtt aaactaaaga atcctattga ttggggtgta ggtaatgtaa
    12241 atttcatatt tatgccagtt tttcaggcta atgataaaga gattgttaaa aatgttcttg
    12301 agatactaaa agatgcagaa tttatgaaca atgccaataa gtgttttgat aaagatgctt
    12361 ttagaaggat tatatttgat aaatttaaac atctgtagcc atagcaaaaa aattgagagg
    12421 agttagagta atgaatgtag atttaatatc attgggttta gaatctaatg attatgaaag
    12481 tgttattgaa gaattaggaa gtataatgtg taaaaaagaa tatgtaaaag aaacatacat
    12541 aaatgctgtc ttggaaagag aaaggacatt accaacaggt ctagatattg gagaaatgtg
    12601 tgttgctatt ccacatacag attcaaagca tgtaaatgag tctaatgtag ctgtagggat
    12661 attgaaaaat cctgtaaagt ttaattcaat gatagaccca aaagatagac ttgatgtaga
    12721 acttgtattt ttattagcag taaagaaccc agattcacaa gtaaaactgt taaaagattt
    12781 aatgtcagtt tttcaaaata taaagttatt aaaaaatata aaaaatgcat caacaaaaga
    12841 agaagtagct aaattattgg actttataga aatttaatgt aggagaattt tataaataaa
    12901 aaattaaaat ttaaacaatc aaagtaagta ttattttttt aataggttta atcttatata
    12961 aaataaaaat ttagagtgaa aaattaggag gaaattatta tgaaacaatt taatgtatta
    13021 tcagtttgtg gttcagggac agttacatca tctatggtag caggtaaatt aaaagaagta
    13081 ctaggtaata aggggatttc tattactaca acagaagcta gaccaacaga agcactaaat
    13141 ttagcacaat caggaagatt tgactttata acatttacga gtccacttca aacaggcgat
    13201 tatggaatac caactataaa tgcatttgct tgcctaactg gtatagggga agaggcattc
    13261 tttgaagaag ttttgactgt tattgaaggg atagataagt aagttttgga gacgtgaatt
    13321 tatagtattt aaatcaatga tttagatact ataataagct aatattaact aaatatttgt
    13381 ggaaaaaaca ttttatatgt tgtcataaat taaaaaatta taaaaatagt cacttaagta
    13441 ttaaattaag cttataaata tttataagta ggagggaaaa agatgttaga atttatgaaa
    13501 tctataatcg atacatttgg ttctgcaatt atagttccaa ttataatatt cataatagca
    13561 aagatattta aagtaactac taagaaatcg tttctttctg ctgtgtatgc aggagttgct
    13621 ttacaaggtt ttacactgat tttaaattca tttacaccaa taataacacc agttatcaat
    13681 aggatggttg agagtacagg agtaaatcta ccagtttttg atgttggttg gcaagcaaca
    13741 tcattggttg cattttcaac aagtgcaggt atgatatatt taggtcttgg tatattatta
    13801 caaacaattt tatttttgat aaaatggaca gacgtgtttc aaccatcaga tttatggaat
    13861 aactattctt atatggtatg gggagcaatg gttataggag taacaggaaa ttttccttta
    13921 ggtatagcat gtatggtttt attaaaccta tatagtttat taatatcaga gttagttgca
    13981 aagagatggt ctagttacta tagatatcct aattgtacaa taatagctat gcataatgtt
    14041 gaagcttctg tgtttgcagt atttgcagac cctatataca ataaattggg attaaataaa
    14101 ataaaattaa atccaaaaga attagagaag aaattagggt ttttaggtga accaattact
    14161 ttaggtcttt ttttaggtat gtttataggt atattaggta atatgacgag aataaatact
    14221 atggaagctt ggggagaaat aatgaaagta ggtatatcta caagtgcagt gatggcaatt
    14281 ttccctaaag tagcaagtat gtttgctcaa gcatttgcac ctataacaga agcagctaga
    14341 aagattatgc aaaaagctgg aaaccgtgag tggtacatag cagtaaatga tgctgttgga
    14401 tatggtgaac ctgcaacttt aatatcaggt cttatattaa ttccaataat gcttgtaata
    14461 gctatggtac ttcctgataa taaggtatta ccagttgtgg atttattggc aattccatat
    14521 atggttcaag gtttagttgc catacataat ggtaatatac caaaagtatt agtttcaggt
    14581 ataatttggt ttggattagg attatatgtt tgtacatcta cagcaccatt atttacagat
    14641 atggctacaa atataggtgt agctatacct gcaggagcaa tgttaataac aagctttaat
    14701 atactaggaa aacctttgat gggattagtt ttctttgcat ttctaagtgc aaatccaatt
    14761 tatataggtc tttcagttgt aatttacttt gttctatggg cattgtttag aaagaacaaa
    14821 acaagtattc ttgattattt agaaaaacaa gcacttaaaa atgtagaaga agagcctgta
    14881 gcagtttaag tttataaaaa tgaaataaga ataattaaag gagtttataa ttgattttat
    14941 ataagtatca gataatacct gagaaatatt taaatgaaat atagacttct ttttatttga
    15001 taatatttgt aattataatg cattgtattt ttatagttga gttttctgta taacctgcta
    15061 tttaaaacat actcttttat atttatacag taatgtggtt taattttata aaatgtaatc
    15121 tctactggta aaagactttg tatataaggc atttgcctga ataaaccatt tatcttttgt
    15181 atggcataga tattgctata tagaagtata tagtatgaaa ggagatattt atatgggaga
    15241 taaaatgaga gcatcagtat tgtataatgt aggtgatgtc agatatgaaa tggtagatat
    15301 accagaaata acagatacgc aagtgcttgt aaatgtaaaa tatgttggga tatgtggttc
    15361 agatttacct aggtctatgg taagtggttt gagtggaaat acaaaatatc cattgatttt
    15421 agggcatgaa ttttctggtg aagttgttaa aattggagaa aaagtaaagc atattaatgt
    15481 aggagataga gtagctgttg caccattagt accttgtgga aaatgtgatt attgtaatga
    15541 aggtaatttt ggcttgtgtg atgattacaa tataattgga acaagagtaa atggagcgtt
    15601 tgcagaatat gtaagagttc cagaagaaca tattttaaaa ctaccagata cattggatta
    15661 tgaaactgct gccggaattg aaccagcaac tatagcttat catgggattt caaagtctaa
    15721 tattagagta ggagatagtg tagttgtgtt aggatgtggt ccaataggtc agtttgttat
    15781 acaatgggca aaggtgtttg gtgcatctaa gattatagca gttgatattt ttgatgaaaa
    15841 attagaactt tctaaacttc taggagcaaa ctatatactt aactcaaaag aagtaaatgt
    15901 aatcaaagaa ataaaaaaaa taacaaatgg tggtgctgat gttgtaatag aaactgctgg
    15961 tagccgattt acacaagaac aatctctatt tgtagcaaaa aagagaggaa atatagtgtt
    16021 tgtaggtata tcacacacag aattaccact tagtgcagac gctactgagt gtatattacg
    16081 tggagaactt accttgaaag gttcttggaa ttcatataca agcccttatc ctggaagagc
    16141 atggactgca acattggatt ttatggaaaa aggtgacatt atttttaaac ccatgatttc
    16201 agataaaata ggtcttaatg aagtgggtga ctttttaagt aaaatgagca agagagaaat
    16261 taatttcaat aaaatcctag tagaaatata aaaatttaat aaaaaattct aaaagcaatt
    16321 aaattttata actatgacat taaataaaat aaaaataatt ttcttaaata aaatatagga
    16381 ggaagaaaag tgttagaaaa tttaaaagaa gaagtattaa aagcaaactt agaattacct
    16441 aagaaaaagc taattactta tacttgggga aatgtaagtg ggatagatag agaaaaagga
    16501 ttagtggtga taaagccaag tggtgtagag tatagtaata tgactgtaga agatatggtt
    16561 gtagttgatt tagagggaaa tatagttgat ggcaaattaa gaccttcttc agataccccg
    16621 actcatctag ttttatataa agagtttgaa gaattggggg gaatagtaca tacacattca
    16681 agttgggcaa caatttgggc tcaaatagga aaatctatac catcttgtgg tactactcat
    16741 gcagattatt tttatggaag tataccatgt accagaaaaa tgactagaga agaaatttgc
    16801 aatgaatacg aaaaggaaac tggaaatgta atcattgaag aatttatggg aagaaatcca
    16861 attcattgtc caggaataat tgtaaatgac cacggacctt ttacatgggg aaaagatgca
    16921 aatgaggctg tacacaatgc agtagtttta gaagaagtcg caaagatggc ttattataca
    16981 gagttaatga gtcctgataa tattatggat aaggttctta tgaataagca tttttcaaga
    17041 aaacatggaa aaaatgcata ctatggtcaa aaatgattat ctggaggtga attatgctag
    17101 actctttaaa aggtaaagtt gtggaaatag caattaaagc agagcattat ggtttatgta
    17161 aagaaaagac tggtaatttt agtattagag ataaggatac tggatatata cttataaccc
    17221 catcaggtgt aggaagagaa gaattaaaag ttgaacatat ttgtgtattg gatttggatg
    17281 gaaatgtaat agaagttgag aaaggtatta agccaagtag tgaggtattg atgcatattg
    17341 aaatatacaa aacaagagaa aatataaatg ccatatcaca tactcattct ttatatgcaa
    17401 cagcattttc agtagcaaag aaaccaatta taccaattgt atatgaatca gttaattatg
    17461 gcggttatgt agatattgct tcatacgaaa gaccaggaac tttaaaatta gctaagagtg
    17521 tttcaaaatt actgatagat acagatgcat gcttattaga aagacatggg ttgacaactg
    17581 taggtaatga tttagacgaa gcattattaa aatcaagata tgtggaagag gtagcagaaa
    17641 tatactatag gtctttagta ttaaatcaat ttaaagaact tgaagtagta gatgttgatg
    17701 agtttaaatc ttggaaatac ccagaagaaa taaagtttta atctaaataa ttttcattga
    17761 agtggttaat taaaatatat gtattattta tatcaattac tatgttttat tatgttaatt
    17821 accgtatttt gcaaatatat ttattacatt ttttcaaaaa aatatagaat ccgaaagcca
    17881 tataaaattg acttgggatt ctatactttt tatttatgca tttttcttta tctgattttc
    17941 tggagaattt atatgttcaa aatatttttt agtttcagat actattatag gtgataatgc
    18001 aagtaaagct ataaggttag gtattgccat aagcccattt acaatatcag caattatcca
    18061 aacaacttct aactttaaga aagcacctaa taacaccata gccacaaata cagttctata
    18121 cccattcatg ccttttacac caaacagaaa ttcaaaacat ctttctccat aataactcca
    18181 tcccaaaatt gtagtaaatg caaataatgt taaactaact gtaagtaata ttggtccaaa
    18241 tgtaggaatt gctccgttaa aagctgcttg tgtcataaca gctccattta ggtctgaaca
    18301 ccacactcct gaaataacaa gagagaatcc agtaagagta cagattataa tagtatctat
    18361 aaaagtacct gtcatagata taagaccttg ttccgctggc cattcagttt tagcagcagc
    18421 agcagctatt ggagcactac ctagaccaga ctcatttgaa aatacaccac gagcgatacc
    18481 atttctaatt gccagtgaca tagttgctcc taaaaaacca ccagtagcag cagtaggtgt
    18541 aaatgcactt ttaattatta acataaaagt ttcagggatt ttattccaaa aagatattaa
    18601 aatgattcca cagattacaa catatataac tgccataaaa ggtactattt tagtagctac
    18661 attagatata cttttaagtc ctccaaatac aaccaaagct acaaatactg ctaatacaac
    18721 ccctgtaatt ctagggtcta tcccgaaact attatttatt gcatctgtta ttgaatttac
    18781 ttgagtaaaa gtaccgattc ctaaaagtgc aactagtata ccacttatag cgaaaaatat
    18841 tgcaagaggt ctccattttt ctcccatacc atttactatg taatacattg gcccaccaga
    18901 cacttggcca tttttatctt tagttctgta ttttattgca agaacacctt ctgaatactt
    18961 agtagccatt ccaaagaatg ctgctatcca catccaaaaa agtgcaccag gtccaccagc
    19021 tttgatagct gtagcaacac caacaatatt accagtacca actgtagcag ctagagcagt
    19081 acataaggct gcaaaacttg aaacatcacc actacctttg ttttcagcag agaatattaa
    19141 cttaagagct cttggcagtt tagttatttg aacaactcca agcttaacag taagtagtat
    19201 accagtacca actaatagta ccagtagggg tggtccccaa ataaaagcat caactttgtt
    19261 taaaatatca attagattca taaaaacact ccctcatttt ttttgcttta cataatcaat
    19321 agaattttta atgaagtaat tgacaggaaa taagtataat ttattaataa tttctagtgt
    19381 aataaaaaaa gctaaatcta aaaaagattc agctctggat gtacactaat tagcaaccaa
    19441 aataaaagta ttagtaaata ctgtttttgg tagataatcc ctgtcctttt gcctgagaga
    19501 ttgagcataa aagccttgcc ccttcggcgc ccataatggg tctctccaga gttccgtaaa
    19561 ttttacagtc ctacttttaa aagtacctga gagtgttact ccttcggtgt gctatatagc
    19621 aactcttcta taaaatctta accggatatt gatttgtcat agcttattgt attaataata
    19681 ttaatataag tttttatgtt ttgtcaacta aaaaattaaa aaacaaagaa aaaatataaa
    19741 aatgtaagcc tatttacgaa ttataaactt agaaagatga gtaatatacg tgaaaatgaa
    19801 ttttgtaata cacatacaaa tgaagtgtaa aataagtttt aaataaaaaa ataataaaat
    19861 cttaaataat taattagata tgattaaatt atggtaatat tatagttacg atattaagaa
    19921 aagttctatt tagttagatt ttaagcgtaa tatgagatta tgtatagaga aaggatgtat
    19981 ttttagatgg gaaaaatatt agtgctagca gaaaaacctt ctgttggaag agatatagca
    20041 aaagttttag ggtcaaaaaa tgaaaaaaat ggttatatag aggggccaaa gtatgtgata
    20101 acttgggcac taggtcattt ggttacactt gctgaccccg aaagttatgg agaaagatat
    20161 aaatcatgga gcttagaaga cctgccaata cttcccaagc accttaagac tgttgtaata
    20221 aaaaagagtg gaaagcagtt taatacagtt aaatcgcaaa tgaatagaaa tgatatagat
    20281 gaaatagtta tagcaacaga tgcaggtcgt gaaggagaac ttgtagcaag gtggataata
    20341 gaaaaatcac aggtaaaaaa acctataaaa cgtctttgga tttcttcaag tacagacaaa
    20401 gcaataaaag acggatttgc aaaattaaaa agtggaaaag aatatgaaaa tttgtattac
    20461 tcagcaatag cacgagcaga agctgattgg attattggaa taaatgcaac cagagctttg
    20521 acaacaaagt acaatgcaca gctttcttgt ggaagagtac aaactcctac tcttgcaatg
    20581 ttgcttaaaa gagaagaaga aataagaaat ttcaaaccta aagagtatta tggattagaa
    20641 ttaattgcta caaagggaaa ttcagatata aaatttattt ggaatgataa aaacaataat
    20701 tcttcaactt ttagtaaaga aaagatagaa tccacattaa aaaaagtaaa aggtgtagac
    20761 ataaaaatat ctgaagtaaa caagtcatat aagaaaaaat attcacctgc actctatgat
    20821 ttaacagaat tacaaagaga tgcaaataaa atgtttggat tttcagcaaa agaaacttta
    20881 tctataatgc aaaaactgta tgaacatcat aaagtactta cttatcctag aactgattct
    20941 agatatttaa catcagatat tgttggaaca ttaaaagata gaataaaagc tgtaaatatt
    21001 agtgattatt caaagatttg taatagatta ttaaaaagca atattatagc taataaatcg
    21061 tttgttgaca attcaaaggt aacagaccat catgcaataa taccaacaga acagaaggta
    21121 ttcatgagtg attttagtga taaagagcga aaaatatata atcttgtagt aaaaagattt
    21181 ttagcagtac tatctcctcc ttttgaatat gagcagacaa ctataagtgc aactgtagaa
    21241 ggagaaatat ttaaggctaa gggaaacaaa gtaataaatt taggttggaa agaaagttat
    21301 aaagatattg aagatgaaga tgagtatgaa agtataccaa atgttttaga aaaagatgta
    21361 cttaaggtca agggattaaa gataacttca ggaaaaacaa atccaccacc atacttaaat
    21421 gagggaacat tattgactgc tatggaaaag aatggtctag ggacagttgc tacaagagca
    21481 gatataatag aaaaattatt caattcattt ttaatggaaa agaaaaataa agatatatat
    21541 ataacatcaa aaggaagaca attattagat ttagttccag tagacttaaa gacaccagaa
    21601 cttacagctt catgggaaaa gaaattattg gatatttcaa agggaaaact tagtaaaaat
    21661 acattcataa atgacatgaa agattattca aaagatattg tactagaagt aaaaaatagt
    21721 gataataaat ttaaacatga taacttaact aaaaatagat gtccagaatg tggaaaattt
    21781 atgttagagg taaatggtaa aaaaggtaag atgttgattt gtgaagatag agaatgtaat
    21841 actagaaaaa ctatatcaca agtaaccaat tcaagatgtc ctaactgtca taaaaaatta
    21901 gaacttcgtg gagaaggtga ggggagaatc ttcacttgta gttgtggata cagagaaaag
    21961 ttgtcttctt tcaacaagag aaagcaagaa gaaaaacaga aggcatcaaa aaaagatgtt
    22021 aatcaatatt taaaaaatca aaataaaaat caagagacat ttaataatcc atttgcagaa
    22081 gcattagcta agctaaaaaa caaataattt taaagttatt attatacaca attataactt
    22141 taactgtata ttagttatat gtactgatat aatatagtgt atagtttagg catgaaagta
    22201 gggctgttaa atggatttaa gtggcatatt taaatactat tgtaaagagt gtgaaaatac
    22261 atggaataat tcgagtgttg aattatttga gaatatagaa acgtatagta aagattcaca
    22321 aaaaaagagg gaaaaagaat tagataaatt gctaaataca atatcagttc atttagagag
    22381 gtatccaagt gatgctgtat tgagaaaaat gtgggtaaaa aagggcgagg ttttcttaca
    22441 aaagacattg gaaaaagaaa atatttttaa gttagaaaaa atggatgtag aggatagaaa
    22501 aaaattttta gatataacaa aacagtttat tagagatgct agaaaatttg atgatgattt
    22561 acctataggt gatattatgc aagctatgag gaatgtatgg atttcaaatg cattacaatt
    22621 attatttggt aaagaagtat attattcaaa agctaacttt gcatatagta tgttatatcc
    22681 atatactgat aattatttag acaatacaaa tatagataaa aatgataaga ttttatttaa
    22741 taactggtta gaaaaaaggc tcctgggaga acacattaaa tctaagaatt atcatgaaag
    22801 taaagtatct caaatgatag attatattga aagtgtatac cctagagaaa agtttacaga
    22861 agtttatgaa tcgttattat taatatttaa aagtcaagta aatagtttaa aacaacatgg
    22921 taaggaaaat catttgtgta aagaagattt attatccatt tctattgaaa aaggaggttc
    22981 atccgtttta gtagatggat atttaataag tggattgatg acaaaggaag aaatagagtt
    23041 ttgtatagga tatggatttt tattacaaat atctgatgat ttacaagata taaaagaaga
    23101 tttaaaatac aaccataaaa ctattattac agagatgtca aaagagggta ctttagataa
    23161 agttgtaaat aaactaataa attttactat tgagttaata gatagtttta aaattaataa
    23221 taaaaataaa tctgtaataa ctatgataaa gaatgattgc ttaatgttaa ttttattttc
    23281 tgtagtttat aatgctgaat ttttttctgt aggatatata aaagaagtag agaaatttat
    23341 tccatataca atagattatt cattagagat tgaagaaaaa ataaaagaaa aatttaaaaa
    23401 tatagatgtt ttaaataatg aaaatgaata taaagaaatg attgatatta tttgtgcaga
    23461 gtagaaaaaa agggagtgtc ttagaataga ctttttagtt tatgaggcac tctttttgta
    23521 taaaatcaaa tgtattttca tcattttaat aattaaaaag agcttatccc tatttgagaa
    23581 agcccttttt aatttaaata aaaatgtcaa ggttaaacta tttaaatgca gtaataatcc
    23641 cattatcatg taagtttttt atgattgtag ctgcgatagg gaatccaaat aatccagcta
    23701 ttccaaatag ttgagctcca acaaacatac acattaatgt tattaaagga tataggccaa
    23761 tttggtgtcc tactaccttt ggttctaata tttgtctaac aacggttata attatgtata
    23821 gtattaaaag tcctattgct aaattaatat ttccattaat gagtgagata atcatccaag
    23881 gtgtcaaaat acttcctgta cccaagactg gcaaaatatc aacaagtgct atgatgacag
    23941 atatagtaaa tgcattttca acctttagga tagtaagtcc aataagtagt tctatgaatg
    24001 tcactgatag tagtattgca taggctttta gtagttttaa aagagcatta atactgctat
    24061 tttttatatt tattatcata aattgacttt tttctggaaa ttgttttaag ataaagttta
    24121 ctattttttg atagtcaaaa gtaaagaaaa aagaagcaat taaagtaata ataagtttta
    24181 acataaatgc aggtgtttta cttgcaatac tagcaatagc attgagtgca tttgttgaaa
    24241 tagaagctac taaagacatc atagattgac ttatattatc ccaaccaagt attaatgcta
    24301 ggttaacatt aggtgtacta gcatctatct ttgaaaataa agtatttagc gttggttgta
    24361 tacttgattt atatatttca ggaagtctaa aaaatacatc acttacactt gcaaatatct
    24421 tcacaccaaa gctaactaat aaaaatatac acaacccata aaaaattaaa agtattacta
    24481 tagaaataag tgtacgattt aaatttgtat tatttgtaat aagtcttata ataggtctta
    24541 agatggatgc aatgataaaa ctgattacaa aaggcattaa taatggcaat aagaatttta
    24601 atacaatata tacaatactt gttattaaaa ctacatatgt aaatttaatt ataaaattct
    24661 tttgcttttg aatatccata atataaaacc tccaatgtat ttgatattat tctaaatatg
    24721 gtc
//
LOCUS       DAEORZ010000048        24281 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEORZ010000048 DAEORZ010000000
VERSION     DAEORZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.048"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            229..546
                     /gene="flgB"
                     /locus_tag="KRH39_001772"
     CDS             229..546
                     /gene="flgB"
                     /locus_tag="KRH39_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBG8511682.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMTTLQYNALISFANGKIAMTKSII
                     SGR"
     gene            554..958
                     /gene="flgC"
                     /locus_tag="KRH39_001773"
     CDS             554..958
                     /gene="flgC"
                     /locus_tag="KRH39_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBG8511683.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKKVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            968..1282
                     /gene="fliE"
                     /locus_tag="KRH39_001774"
     CDS             968..1282
                     /gene="fliE"
                     /locus_tag="KRH39_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBG8511684.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNKEEKKNFEDVIIDTID
                     KVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNKV
                     QI"
     gene            1307..2857
                     /gene="fliF"
                     /locus_tag="KRH39_001775"
     CDS             1307..2857
                     /gene="fliF"
                     /locus_tag="KRH39_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBG8511685.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTSGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVNENNQPINSGISVDKNKASEQALNKQLEKSILEILEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2870..3940
                     /gene="fliG"
                     /locus_tag="KRH39_001776"
     CDS             2870..3940
                     /gene="fliG"
                     /locus_tag="KRH39_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBG8511686.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSMSGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAAEALREEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3933..4664
                     /locus_tag="KRH39_001777"
     CDS             3933..4664
                     /locus_tag="KRH39_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBG8511687.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKINAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            4668..5987
                     /gene="fliI"
                     /locus_tag="KRH39_001778"
     CDS             4668..5987
                     /gene="fliI"
                     /locus_tag="KRH39_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBG8511688.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRGIFE"
     gene            6019..6465
                     /gene="fliJ"
                     /locus_tag="KRH39_001779"
     CDS             6019..6465
                     /gene="fliJ"
                     /locus_tag="KRH39_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBG8511689.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSIGKNNDSVQNQKITINYLLALNNSIMDLSEKLDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6493..7698
                     /locus_tag="KRH39_001780"
     CDS             6493..7698
                     /locus_tag="KRH39_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBG8511690.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNDKFEDNLYKTTDHKKV
                     KDRRETSKQNVSSDRPENSDNECHLDEDKSNHLEDENTYEKDCSNNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            7716..8324
                     /locus_tag="KRH39_001781"
     CDS             7716..8324
                     /locus_tag="KRH39_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBG8511691.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            8351..9334
                     /locus_tag="KRH39_001782"
     CDS             8351..9334
                     /locus_tag="KRH39_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBG8511692.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQIYAFQNPAGLDADGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9347..9550
                     /locus_tag="KRH39_001783"
     CDS             9347..9550
                     /locus_tag="KRH39_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBG8511693.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9550..10368
                     /locus_tag="KRH39_001784"
     CDS             9550..10368
                     /locus_tag="KRH39_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBG8511694.1"
                     /translation="MKKTDSLTFIGLILSIVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLMKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMEIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIVLNPIGYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNERLETMKSDKNTERAMKDGA"
     gene            10358..11053
                     /locus_tag="KRH39_001785"
     CDS             10358..11053
                     /locus_tag="KRH39_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG8511695.1"
                     /translation="MARKKNKKKDEINPDAWLATYSDTITLILTFFVLLYATSVVDME
                     KFKDIADALKGQFSGTSVLGTGSSTSGKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11065..11508
                     /locus_tag="KRH39_001786"
     CDS             11065..11508
                     /locus_tag="KRH39_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBG8511696.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLNNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11523..11897
                     /locus_tag="KRH39_001787"
     CDS             11523..11897
                     /locus_tag="KRH39_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliO/MopB family protein"
                     /protein_id="HBG8511697.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMNKIKDLTKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11898..12563
                     /gene="fliP"
                     /locus_tag="KRH39_001788"
     CDS             11898..12563
                     /gene="fliP"
                     /locus_tag="KRH39_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBG8511698.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKKFLLKQTRQADLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12576..12845
                     /gene="fliQ"
                     /locus_tag="KRH39_001789"
     CDS             12576..12845
                     /gene="fliQ"
                     /locus_tag="KRH39_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBG8511699.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            12857..14671
                     /locus_tag="KRH39_001790"
     CDS             12857..14671
                     /locus_tag="KRH39_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBG8511700.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     VPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDIAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            14680..16755
                     /gene="flhA"
                     /locus_tag="KRH39_001791"
     CDS             14680..16755
                     /gene="flhA"
                     /locus_tag="KRH39_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBG8511701.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMIIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTVGDGLVSQIPALLISTSAGIIVTRASTDENFGDLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELISNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16730..17746
                     /gene="flhF"
                     /locus_tag="KRH39_001792"
     CDS             16730..17746
                     /gene="flhF"
                     /locus_tag="KRH39_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBG8511702.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKLIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17743..18621
                     /locus_tag="KRH39_001793"
     CDS             17743..18621
                     /locus_tag="KRH39_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBG8511703.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18635..19336
                     /locus_tag="KRH39_001794"
     CDS             18635..19336
                     /locus_tag="KRH39_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBG8511704.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19352..19774
                     /locus_tag="KRH39_001795"
     CDS             19352..19774
                     /locus_tag="KRH39_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG8511705.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            19759..19944
                     /locus_tag="KRH39_001796"
     CDS             19759..19944
                     /locus_tag="KRH39_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG8511706.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19985..20758
                     /gene="flgG"
                     /locus_tag="KRH39_001797"
     CDS             19985..20758
                     /gene="flgG"
                     /locus_tag="KRH39_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG8511707.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20779..21540
                     /gene="flgG"
                     /locus_tag="KRH39_001798"
     CDS             20779..21540
                     /gene="flgG"
                     /locus_tag="KRH39_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG8511708.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21563..22444
                     /locus_tag="KRH39_001799"
     CDS             21563..22444
                     /locus_tag="KRH39_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG8511709.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22447..22821
                     /locus_tag="KRH39_001800"
     CDS             22447..22821
                     /locus_tag="KRH39_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG8511710.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22853..24256
                     /locus_tag="KRH39_001801"
     CDS             22853..24256
                     /locus_tag="KRH39_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG8511711.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNNVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVKN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
BASE COUNT        10042 a         2590 c         4571 g         7078 t
ORIGIN      
        1 agcaactttt cgaagaaata tttaaataca tattaaaatt tttttatatt ttcatgggac
       61 ttcattgaaa aaatattagt tttcttacta aaatgataca ttattgtatt aatgtaatgg
      121 aaatgcgatt aatttaaatg aataaagaag tcattttttg taatatagca acttcatttt
      181 tttattaata agcaaaataa ataaaatata ttttgaggag gagtgagtgt gaatacatat
      241 gacattataa aaatgggatt agatgcaact aatttaagaa gtcagacaat agcaaataat
      301 atagcaaata taaatactga aggatataag agacattatg taacttttga agaaacatta
      361 aatgatgcta tgcccaaaat agaagaaaaa acagataatt ctaaaggatt aagagtggat
      421 ggcaataatg tagatataga agaagaaaaa gtaaaccaag caatgacaac attacaatat
      481 aatgctttga taagttttgc aaatggaaaa atagcaatga ctaaatcaat aataagtggg
      541 aggtaattaa aaggtgagtg tgttcagtgg aatgagaata tctgctagtg gattaacagc
      601 agaaagcttg aaaatggata tcatatcgtc caatgtagct aatataaatg ccacaagagg
      661 tccaaatgga gaagcatata ggagaaaagt tgtttcattt gaagaaaact atgataataa
      721 attaggtatg ctaggagtaa aagttacatc tgttgaagat gataaatcac ctcttaaaaa
      781 agtatatgac ccatctcatc cagatgcaaa gccaaatgga tatgtagaat atccaaatgt
      841 aaatatattg gtagaaatga cagatttaat aacagcttca cgagcatatg attctaacgt
      901 agatacattg aatgctcaga agtctatgat atcaaaagct ttggaaatag gaaagtaggg
      961 ataaattatg ccaattacac aaattaacaa tttaatgcaa ggtgcaaagt tagataattt
     1021 gattggagaa acaaaaccat taactcttaa taaagaagaa aaaaagaatt ttgaagatgt
     1081 aataatagat actatagata aagttaattt tcaacaagtg acagcagatg atatgaaaga
     1141 aaaatttata aaaggcgaag atgtttctat gcatgaagtt atgttaatag gtcaagaagc
     1201 acagctatct atgcaatatt taatagaagt tagaaataag attcatgatg catatcaaga
     1261 aatgaataag gtacaaattt agccttttga acaggagtaa taatcaatga tagccagttt
     1321 taaaaataag attaaagagc aagttgctaa atctaggaca aagataagtt ctctaaaaaa
     1381 gggaactaaa atagcattag gtataggggt tattgctata tgtttagcgc tagtatttac
     1441 agcaatatct gcgagcaaaa ataagtatgc agtcttattt tcagaactag atgttcaaga
     1501 tgctgcagat atatcagcag aattagagtc taaaaagata gatactaagg taaagggaag
     1561 tacgatttat gtacctagaa cagaagttga taagttaagg ctggagttag caccaagtct
     1621 tacaagtggt tctaaaggat ttgcactaat ggatgatagt tcatcaatag ggcttactga
     1681 tgaagagttt cagattaaga agcaaagaat gatacaaggt gaaatagaaa aaacaataaa
     1741 gagttttcca caagtggaag acactagagt acatataaca tttggagagt catcagtatt
     1801 tgagaaggag gctgttccag ggagtgcagc agtttatttg atgctaaaac cagggacgac
     1861 tttagaaaaa aaccaaataa agtctgttat ggcacttata tcaggaagcg ttacaaatat
     1921 accagagaaa aatgttcagg ttattgataa caaaatgaat ttattatctg aaggtatttt
     1981 ggaggatgaa aatgttaatg aaaataatca accaataaat agtggcatta gtgttgataa
     2041 aaataaggca tctgaacaag cattgaataa acaactagaa aagtctattt tagaaatact
     2101 agaaccaata tttggtaagg gaaaggtgaa agctacagta aatgctgatt taagttttga
     2161 tacagctgaa aaaactgaga taaaaattga ccctgataaa gttgcaataa aagagacaag
     2221 gtcgaaaaat tcatctaaat ctgctacaaa taatgttcaa ggtgtagatg ctaacatgaa
     2281 taaccaagga aataacaatg gtactaatac agaagatagt ttagatgaga gttttgagta
     2341 tgaaacaggt aagattgaaa ctcatactat agttgcgcct ggagaactta atagattgac
     2401 agcttctgtt gcaatagatg gaaaaatagc taaaggtgtt caagctgatg tagaggatat
     2461 tgttaataat gctataggta tggatggagc gaggggagat actcttagcg ttgtttcaat
     2521 ggtatttgac ccagaaggta agaaagaggc taaacaagag ctggaagaga ttaaaaaaga
     2581 aggatttaaa aagaacataa taaaagcagt tattggagta gtggcagtag tagcaattgg
     2641 agctattgga tacttgatat ataagaaaag aagtgaacga gaagatatgg attatgaaga
     2701 tgaagattat atttctaaat ctgattctgt tgatagaaaa gataatcctc tattaggaga
     2761 tgacccagaa ctgacattag aggaagctgt taaaatgtat gcagaagaaa agccagacca
     2821 agtaacagag ataataaaaa cttggttaaa tgagtaaggg ggatagaaag tgtccacgga
     2881 aattacaaat gttaatgcgt ttgacttaag tgatataaat gagagtgata agaaagagaa
     2941 gatagaaggt gagggtttaa aaaaagctgc tgtattatta atgacattag gaccaaatgt
     3001 ttctagtgat ataataaaaa gcttgcctga taaacaagtt caaaaaatag gtttggaaat
     3061 tgcaaatatt tcttcaatag cttcaaaaga aagaagagaa atcttaaaag agtttgtaga
     3121 aataaatagg gcaaaagatt atgttataga aggtgggctt gaatatgcaa gagagttgtt
     3181 aagcaatgct ttaggaaatg ccaaagcaaa caaattactt gaaggtatat caataaatac
     3241 aagcacaaag ccttttaaac atattataaa actagatgta aagaaaatat tagaaatact
     3301 taggaatgaa agtgcacaaa ctatagccgt tttacttgca catatggagc cagaaaatgg
     3361 agcagaagtt ttatcatact tagattttga tttacaaaat gaagtagcta tgaggataag
     3421 ctcaatgggt tcaatatctc cagaagtaat aaaagtaatt gaaagttcat tagaagataa
     3481 attacactca atgagtggtg atgattttaa ggattatgga ggagtgtcta ccttagttca
     3541 aatattgatt aattctgata gaaaaacaga aaagaatatt ttgggaacta ttgaagaaca
     3601 agacccggat ttagcagctc aaattaaatc tcatatattt gtctttgaag atattgtcaa
     3661 gttagatgat ataagtatac aaaaagtgtt aaaagaagtg actatgaaag atttagtata
     3721 tgccttaaaa ggtgtggatg atgagatttc agatgctata tatagaaatc aatcttctag
     3781 ggcagcagag gcacttagag aagaaatgga aatggttggt gtcataaaag ttacacaggt
     3841 tgaggctgct caacaaaaga tagcaaatat agtaagaaaa cttgaaagag aaggtgccat
     3901 aactattgta aggaattttg gtggtgactt tgttgaataa taagattatt aaaagtaaaa
     3961 aagcattata taaaggagtg ctagatttaa ccataataga taaagttaga gtgggagaat
     4021 caaatgtaaa taaagaaaat gattgtagat ttagggatga ggtaataaaa gaagctatag
     4081 aatctgctga gataaaaaag aatgagatat tagaagaagc aaaaataaat gcacaaaata
     4141 tagaaaagca agcatatgaa aaagggtata gtcaaggtca aaaaaatgga tatgaggatg
     4201 gatataaaga gtcatatgat gaatataccc aaaaagctaa agatgaagca gattttataa
     4261 aaaatcaggc agacttaata ctattacaag ctaatgagaa gatgactgag tacataaaag
     4321 aaaaaaataa agagattgta agacttgcta ttaatatggc cagttctgtt ttaaaaaagc
     4381 atttcgaaga tgaagaatct atgagtgatt tgttaaagca aattattcaa gattatgaag
     4441 gaagtagttc atttatagta agatgtaatt ctttctataa agataatata gagaaagagt
     4501 ttgaattatt caaagaggaa acatcactaa aatcaagtgt atttgttatt gcagatgata
     4561 gtatagataa aggcaatgct attatccaaa aggaaaatgg aagaataata gttggtattg
     4621 attgtgctat ggaaaaacta aaagaagaat tgcttggtga ataaagtatg tataaaactg
     4681 atatatctat agattttgac aaaataaata agaaaataga aaatttagaa ctcactatat
     4741 cagaaggtag agtaaagaag ataataggac ttactgtaga agttgaagga ataaaagctt
     4801 ttgttgggga gttatgtgtt atatacaatc aagtcaataa accagtgaat tgtgaagtag
     4861 tgggttttaa agataaagaa gtcatattaa tggcgttggg agagttaaca ttgatagctc
     4921 ctggttgtaa agttatttct aaggggatac ctttaagtgt tatgtgtagc gataatttac
     4981 taggcaaagt acttgatggt cttggaaatc ctatagataa ctcagatgct gttttaggag
     5041 atagatacaa cttgaacaat gaacctcctg atccaatgaa gagaaaaaag atacgaaata
     5101 taatggaaac aggtgtaaga gcaatagatg catttactac ttgtggtgaa ggacagagaa
     5161 tagggatatt tgcaggaagt ggagtaggta aaagtacgac tctaggtatg attgctagga
     5221 atgcaaaagc agatgtaaat gtaattgccc ttattggaga aagaggaaga gaagtattag
     5281 attttattga taaagattta ggtgaagagg gaatgaaaaa atctgttgtt gtatgtgcaa
     5341 catcagataa agctcctttg gttaggctaa aaggtgctct tactgctact gcaatagcag
     5401 agtattttag agaccagggc aaaaaagtaa tacttatgat ggactcggta actagatttg
     5461 ctatggcaca aagagaagtt ggtgtagcaa taggagagcc accagctcaa aaaggatata
     5521 caccatctgt gtttgctatt ttaccaaaac ttatggagag aacagggaca tcagataaag
     5581 gttctataac tgcattttat acagtattag ttgatggaga tgactttaat gagccaatag
     5641 cagatacaac aagagggata cttgatggtc atatagtttt atctagagac ttggcaaata
     5701 agaatcatta tccttctata gatgtattga acagtttaag cagacttatg aatgaaattg
     5761 cttcaaaaga agacataaaa atagcatctt ttgcgagaga tatgcttgct gagtatagag
     5821 aagcagaaga tttgataaat ataggtgcat atgcttcagg tacaaacaaa aaaatagatg
     5881 aagcaatcta ctatcatgag catattatta attttttaaa acaaggaata aatgaaaaga
     5941 gctcttttaa tgaaactata agtagtttaa gaggaatctt tgaataaaca atttaaaaaa
     6001 atatttgaat aggttgtgat ggaattggat aaaaatttta agtttagatt acagaaagtt
     6061 ttagacttaa aaataaaaga tgaagaagag ataaaaatgg aatttgctaa gattcaacaa
     6121 aaaaaaatag atatagaaac taatttagaa aacttagaaa gtaattacag caaatattct
     6181 ataggtaaaa ataatgatag tgttcaaaat caaaaaataa ctattaacta tttgctagct
     6241 ttaaacaact ctataatgga tttatcagaa aaactggata agtcgacaaa tgaacttgaa
     6301 aaagctagaa aacagcttat aagcaaacag atagagagaa agtcattaga aaaattaaaa
     6361 gaaaaaaaat atggacagta ttataaagaa gaacagttaa aagaacaaag cactaatgat
     6421 gagtttgcaa gcatgagtta tttgagaaat agacaagttc tataaaatag taggaagtga
     6481 ggatattatt gtatgaagat acaagcagat tcttataata ttattaaaga tattgggaat
     6541 agaaaaaact catctaagaa taaaaatata aaaaatgata aatttgaaga taatctatat
     6601 aagacaacag accataaaaa ggttaaagat agaagagaaa caagtaagca aaatgttagc
     6661 tcagatagac cggaaaatag tgataatgag tgtcatttag atgaagataa aagtaatcac
     6721 ttagaagatg aaaatacata tgaaaaagat tgtagtaata ataatgaagc tttgcagcaa
     6781 ttactgaatc ttttaaaaga gaattataaa aaagataaag atataaacac tatattgaat
     6841 aatataaaaa aactaagtat atcagatgaa ttaaaacaag aacttaagct taataaaaat
     6901 atattaaata agattgcaaa tcaaaattca gagctaaata aagctaatat aaataactct
     6961 aatgatatag aaaatttaga catagttaaa ttaagagaac ttttggagga taatgctaat
     7021 aaagatattg aagtaattac taaaactaca gaaaatgata ctaaaaatat agatttgtat
     7081 agttttgata gcaatagaat ggataatctt aactcaaata aacagaaaga tgatagctct
     7141 gatattttgg aaaaactagc aggggtaaat ggaaataaga gtgggaattt tggacaagta
     7201 ataaataaaa atgttgatat gaataaaatt aaaaatgata atgtcaattt aaaacagata
     7261 gaatcaaatg taatggatga ctctataaaa gctattaagc atatgaaaac aaatgatata
     7321 caagaattga caattaaatt aagaccaaaa gaattaggag atatgaatat acaattgtta
     7381 aaagatggtg aaagtatgag agctgttgtg actgttttca ataaagatgt ctttgactct
     7441 ataaataaaa acatagcaga tttaaagcaa catcttgaac ttactaatgt aaatataaaa
     7501 gatgtatcag tgcaaatgca cagcgataat acaaatacat ctgatacctt tgataaagca
     7561 tttgaacaac agaatagaca aaataaccaa gaaaacatga gtaagaagaa tacaaatgat
     7621 aagcataata atgtagtcat agaagatgac attaaagaaa atactattga tgatgataga
     7681 gtagatttac tagcttagta agggggaagt attgcatgta cggaatagca acggctttgg
     7741 caggaactca gggaaactat aggtcaaata acttaagcca acttgaaaga aatattaatg
     7801 atattgccaa taaacaagtt agaagcaaag gaaatcagac agtaacagac aatgggactc
     7861 caattatagg acaaggtgat gatgctgata aagacttgtt tatgaagata cttgtggcac
     7921 agatgggtaa tcaagaccca atgaatccgc aagacccaac agaatatgtg tcacaattag
     7981 cacagtttgc aactttagaa aagatgtcag ctatgaatga gaagctagat gtacttttag
     8041 ccttaagtaa ttccactcta ataaactctg ctttgagtat ggcaacatca ttaattggta
     8101 aagaagtaga agtatctggg gaagagaaag aagatgaaaa cattgtagga gtagtaaagt
     8161 ccacatatat aaaagatggg gaagtacact tagaattaga agtagatgga agtgacaaaa
     8221 ctgtaggtgt taaatatgaa aaattactta aaataggaga ggtagatgaa aattcaggag
     8281 aagaaaatga ttcttcagaa cacgaatctc cagaagaagc ataatattaa atgagaaaag
     8341 aggtaaattc atgataaaag caatgtattc aggtgtaagt ggaatgaaag caaatcaaac
     8401 aaaattagat gtaataggta acaatgtagc caacgctgga actacttctt ttaagaaaag
     8461 ttcagctaga ataacagatt cattttatca aactgtacta tatgcaagtg ctccaacagc
     8521 tgcattagga ggaacaaact taggacaagt aggtgtaggt agtaagatat caagtataaa
     8581 taaagatatg gtacaaggaa atgtacaacc aactaataga ccatctgatt taatgataga
     8641 tggagatgga tatcttccag tagtaagaca aggaacagtt atgtatacga gagatggttc
     8701 ttttaattta gatacaggat ttaaagcagc tgatggtgct ttgaagaata ttaaaggtgg
     8761 aagacttgta aatggtgatg gatatatgtt acaaggtatg atttatgatg gggaatataa
     8821 agctgatact ggagaatttg ttaaaaaggg tgatgctaaa ggaaaagagc ctattgtaat
     8881 accacttcaa agtataagtc ctaaggatgg aacagttcaa aatgtactag agtataatat
     8941 atcaaaggat ggaacagtag aatttttatt gagtgatggt caaagaactc aatttgtcta
     9001 tgatatacct gatggtgcta aggtaggaga tacacctgct ggaatagata aattaaaagg
     9061 tacaatacaa aaagtacaaa tatatgcatt ccaaaatcca gcaggcctgg atgctgatgg
     9121 aggaaacttg tttaaaccat cagcaaactc aggagaccca caaatagcag gagctacagg
     9181 cacaataata caaggggcaa tagaagcttc caatgtagat atagcagaag aatttacaga
     9241 aatgataata gcaagtaggt ctttccaagc aaacagtaaa ataatatcga catcagacga
     9301 gatacttcaa gaaataataa atcttaagag atagtaggta catattatga taagagtaac
     9361 ggatttaaac ggaagaaaat acatattaaa ttcagacctt atagtgagga ttgattctgt
     9421 accagaaagt aggattctcc ttacaacagg agaaaaaaat cttgtaaaag agtcagtaga
     9481 tgagatagta gataaagtaa taaagtataa aagaaaaata aatgtaggtt atattaggag
     9541 cgaaaaataa tgaaaaaaac agattcactg acatttattg gactaatatt atctatagta
     9601 ttagttttag ttggtgccgc aaaaggaagt agttcagggt taaagaattt ttttgacgtc
     9661 tcatcaatat taatcacagt gcttggttca tttggagctc taatgattac atttactata
     9721 gatgatataa aacttatgaa aaatgcatta caatactcat ttaagacaat gagtgtatca
     9781 aaattagatt tactagaaca atttaaaaca ctttctaaaa aagctagaaa agaaggtctt
     9841 ttgtcaatag aaagtgatgt aatggaaata gaagatccat ttatgaagaa aggattagag
     9901 ttatgtattg atggattgga aatcaatgag ataaggtcta tcttagaatt agagatagat
     9961 tctgttgaag atacaaataa cagatactct aaactattta aaacttgggg aacctttgca
    10021 cctgcatttg ggatgttggg gacactaata ggtctaatac aaatgctagc tgaccttaca
    10081 tctccagact taatagcatc tggtatggga aaggcattaa ttacaacgtt ttatggttct
    10141 ctacttgcca atattgtttt gaatccaatt ggatataata tagacgagaa gactgaaaaa
    10201 gaaatttatg taaaagaaat gatgttggaa ggtataataa gtatacaatc tggagagagc
    10261 agtatagtag ttgaggaacg tttagccaca tacctttcaa ataatgagag gttagaaact
    10321 atgaaatccg ataaaaatac agaaagagca atgaaagatg gcgcgtaaaa aaaacaagaa
    10381 aaaggatgag ataaatccag atgcatggtt ggcaacttat tcagatacaa taacactaat
    10441 tcttacattt ttcgtattat tgtatgcaac atcagttgta gatatggaaa aatttaaaga
    10501 catagcagat gcgttaaaag gtcagttttc tgggacaagt gtacttggta caggttcaag
    10561 tacaagtggg aaatctccaa tagacagtgt tttagtggat aaaaatgata aatcaaattt
    10621 agaacaggat ttaaaagata aggttaataa ttctaactta caaaatagtg taacagttaa
    10681 agatgatagt agagggattt tattagagtt agatgattca attttatttg actctgggat
    10741 ttctgagtta aaagtagata gtaaagctac tcttaataaa gtatatgaca tgataaagat
    10801 gatgaaaaat aagatagtta tagaaggcca tactgataat gtacctgtaa aatcaagtac
    10861 acatgaatcc aattgggatt tatcttctag tagagcagta agtgttgtaa gatactttgt
    10921 agaagaaaag ggcatggacc ctaaactatt ttcggcaaca ggatgtggag agtatcaacc
    10981 tttggtttca aatgaaacag caaagggcag ggctgagaat agaagagtta atatacttat
    11041 aactattgac taggaggaag aggtttgttc aaaaataaaa aaattattat aattattttg
    11101 atactgatta ttttctcagc gggagcaggt ttttttatat taggagaagg tggaaataaa
    11161 caaattgaca agattgcagg tttgtttatt ccaacaggaa aaggtaaaga tgaagtaaaa
    11221 actgaaaatc ttgacttaga agagatggta ttgaagctag ctgatgaagg tgtaagatat
    11281 ttaaaagtat caataactgt atcttatgat tcttcatata aagatataga aaaaaatacg
    11341 cctttaatta gggataatat tataaattgt tttatggcaa aaagagcaga tgattttact
    11401 gctgataatt taaataatat aaaagaagaa attaaaaata cattaaatat acaactaggt
    11461 gaaaatgtaa ttcaaaatat atatttcaca aatatagtag tgcaataaaa ggatggtaag
    11521 acatgatact ttatttatta aaaatattaa tatgtatccc aataatttta atattgatag
    11581 tcttatctct aaaaattagt caaggaactt tattaaaaaa atataatgat aaatacataa
    11641 aaatactaga tattgtaaat ataagtaaaa acaatagcat aataatttta aaaataggtg
    11701 aagaaggatg cattatggca tcttcttcct ctagcatgaa taaaataaaa gatttgacca
    11761 aagaagaaat attaaaaata gaaaacgaca ttaaagaaag aaattttaaa attcaagatt
    11821 ttaaaatgga aagaattaaa aagaaatatg atttcagtag atatattgga aaatttacaa
    11881 aaaaggaaga taagtaaatg gaactattat caaaattacc tttggctgat gtaccatata
    11941 caagttcaat gcaattgttt ttatttctaa ctgcattaat gtttttacca tttgtaatgt
    12001 tcatgatgac aagctttgtc agaatagtta taagtttatc atttttgaaa tcagcattag
    12061 gagcacagca agcaattcca agccaaattt tggtagggct tgcaatagta ttgacaatat
    12121 ttataatgag accagtatta aatgagataa acgaaaaggc gttacaacca tacatgaaag
    12181 aagaagttac tatggaagaa gctatgaaag aagcagaagg acctattaaa aagtttttat
    12241 taaagcaaac cagacaggca gatttagatt tatttgtaga acaggctggt ttaaaagaaa
    12301 agaaattaac tcgtgaaaat ataccactat ctgttgtagt tccagcattt gctataagcg
    12361 aactaaaaac tgcttttcaa ataggttttt taatatatat accatttttg ataatagacc
    12421 ttgttgtggc aagtgttttg atgtctatgg gtatgtttat gttgcctcca gtcatgatat
    12481 cattaccatt taagttatta ttatttgtaa tggtggatgg atggaacttg atagtaaaaa
    12541 cattgatatt gggatttgga taggagaaaa atagtatgag tgaatttatg ataatgagtg
    12601 ttcttaaaaa tggcatgttt acaggagcta tgattgcagc tccagtactc attgtttctc
    12661 ttgtagttgg gcttttaata agtgtgtttc aagctacaac tcaaatccag gagcaaactt
    12721 tgacttttgt accaaaacta atagctattc ttttagtagt agctattggt ggaagctgga
    12781 tgttacatac tcttattagc tttacttctg agatattcaa tatgatatct cttataacta
    12841 aataagtagg atacatatga tttttgtatt tattagaata gtagcattct ttggaacact
    12901 aagaatacta tttccagctg gtacacctgc tttatttaag tcactctttg caatcattat
    12961 atcaatattg atttcaagta ctatgcaaat tgaatatgca acaaatatag acaacattgt
    13021 attgtttacc ttatatggta taaatgagac cataactggg attatgcttg gatatataac
    13081 caacctatgt ttttatagta tcagaatggc gggttctatg atggaccaac agatgggatt
    13141 atcaatgatt aatatgtttg acccaaattc aatgactcag acaactttaa ttgataatct
    13201 tatgaactgg acagcactta tgatattttt tagcatggat ggtcatcatg ttttaattag
    13261 aggtataagg tatagttttg agttaattcc aataggaaaa ccatttgtag ataataacat
    13321 agattatata ataaacatat ttgtgcagtg ctttttaact ggattcaaaa tagcaatacc
    13381 tattgtactt tgtctgctta tggcagattt tattttaggg cttatatcaa gaagtgtacc
    13441 tcaattaaat gtaatgatag ttggtatgcc attaaaaata ttggttggta ttgcattatt
    13501 tatcatatca gtacctttaa ttgtcaatca aataagtcat ttattaagtc aaataccaaa
    13561 gatgtatgag ggaacttttg cactagctcc tatgtttttt atgggttcag cagataaaac
    13621 agaagaagct actccaaaga aaaagggaga acaaagaaag aaaggtaata tagcaaagag
    13681 tagagaatta cccgttgcaa tgactctact tgcattcacc ttattagtac ctacattatt
    13741 ttcatatgta gtagatacac ttaaatcttc gcttaattat tttttgagtc tagattttta
    13801 tatgaacata aattattcaa acctagagaa attagttata gcaggattaa tggatttttt
    13861 taagatattt ttaccaatag ctattccctt tttagtttta ggcataatag caaacctatt
    13921 tcaggtggga atactgttta ctggtgaaac tttaaaacca aatttatcaa aattaaatcc
    13981 agttagtgga ttcaaaaaca tgttttctat gagaagtttg tcaaccttaa taaaggatat
    14041 agcgattata tctattttgg cttatatagg atatacattc ttccaagata attatttaga
    14101 tatattaaag ctaggtaaca tatacctacc aactttaatg tacacagtta aagatttagt
    14161 atattcaata ttgagtaaga tttgtgtagc tatgatagct atagcagttg cagattatgt
    14221 ttatcaaaga tacagtcata aaaagcaact gagaatgaca aaacaagaag ttaaagatga
    14281 atataaaaac tcagaaggag acccagaagt aaaagctaag ataaaacaaa aacaaagaca
    14341 aatttcgtct caaaggacaa tgcaagcagt accaagtgct actgtaatag taaccaatcc
    14401 aacccactta tctatagcag tgagatatga aaaagggaaa gaccaagctc cagtagtggt
    14461 ggcaaaaggt gcagattatt tagcttttaa aataagggaa attgcaaagg gaaatgacat
    14521 ccctataata gaaaacaaac ctattgctag gttgttatat aagcaagtag aaatagacca
    14581 agaaattcca gaggacatgt atcaagcttt tgcagagata ttagtggcag tatataaaat
    14641 caaaaataga tacaaagtcc ccaaaaggta ggataagtaa tgaataagtt taatttaaag
    14701 gaaaatatgg acgtaatagt ggcatttggt gttgtaggga ttgttcttat gataatagtg
    14761 ccattaccta catttatatt agatatttta ctggcaatta acataagttt atctgtgcta
    14821 attttactaa tgacattatt tacaacaaat gtacttgatt tatctatatt tccaacagtg
    14881 ttacttgtga caacactatt tagattggga ttaaatcttt catcaactag attgatttta
    14941 acacagggat atgcaggtga agttatagag gcatttggaa gttttgttgt aggtggaaat
    15001 tacgtagtag ggataattat attcttaatt atattaattg ttcaatttgt tgttataacc
    15061 aatggttctg gaagagtatc cgaagtaaca gctagattta cactagatgc tatgccaggt
    15121 aagcagatga gtatagatgc agatttaaat gcaggagtta tagatgacaa aactgctaga
    15181 aaaagaagaa aagagcttca acaagaagca gatttttatg gttctatgga tggagcaaat
    15241 aagtttgtaa agggagatgc catagcaggt cttatagtaa cggcaataaa cataataggt
    15301 ggaattgtaa taggagctgt aatgctgaat atgactctta tgggtgcagc acagacatat
    15361 gtaagactta ctgtaggaga tggtcttgta agtcagatac ctgctctatt aatatctaca
    15421 tcagcaggta taatcgtgac aagagcttct actgatgaaa actttggtga cttagtaggt
    15481 aaacagttaa ccgcaatacc aaaagcagta atgatgactg gttgtgttct aatcgtactt
    15541 ggtatgatgc ctggattacc taaaccagca tttttttcac taggtatagg agctatagca
    15601 gcaggttact ttttaggaag agaaaagaaa gatactttag aagacttgga gtttgaaaaa
    15661 cctgatatgg taaacccaga taaaaatgaa gtagaagatg taacaaactt aataaatgta
    15721 gaatcaatag aagtagaaat tggatatggt ttaatatctt tagctgatga atcagcagga
    15781 ggagatttac ttcagagaat agcatcaata agaagacaat gtgcaataga catgggtata
    15841 gtcgtacaac ccataagaat aagagataac ttacagttaa atccaaacca atacaatata
    15901 aaaataaaag gaaatgtagt tggtgggtat gagataatgc ctaatatggt tttatgtatg
    15961 gatcctatga atcaaggatt tagtatattt ggaataaaga caattgaacc tacttttgga
    16021 atagaggcac tttggataga aagtgacaag gtagaagatg cagagttaaa gggttataca
    16081 gttgtagatt catccacaat acttataact cacttactag atataataaa atcaaatgca
    16141 cacgaattac tgggaagaca agaagttaag actattatag atgcagcaag agaaaattat
    16201 agtgctgtag tagatgaatt gataccagat ttgatgacac ttggtgaaat tcaaaaagta
    16261 cttaaaaatc tgttaagaga aaaagtaaat ataaaggata gagttacaat acttgaaacg
    16321 cttgcagacc agtctagaaa tacaaaagat atagagcttc ttactgaata tgtaagaata
    16381 gccttggcta gaagtatttg taataattta gttgatgatg ataagacaat tgtagtggct
    16441 acattgtctc cagaaactga agagttgata tctaataatt tacagaggtc tgtaaatgga
    16501 acttatccag cagttgaccc agaaaataca aataaaatat ttgaaagtat acaagaagta
    16561 atgagcaatg tatatttcaa tcataatata ccagtaatag tagtatcacc aaagataaga
    16621 gctccattta gaaaattagt tgaaattgta tatcctaatt tgactgtatt atcattaaat
    16681 gaaataccta acgatattaa aatcaaagca gaaggtgtgg tgagcatata tgatgacgaa
    16741 aaagtataca gctaacacaa ttcaggatgc tatgaatcta gccaaaatgg aattgggtga
    16801 caatattaca ttaatagata aaaaagaagt aagaaagtct ggaatacaag gtatcttttc
    16861 aaagaaagat attgaactaa ctattggatg ggaaaagaga gaaaacgtag aacaaaaaga
    16921 tttaaaacga gagatagaac aattaaagtt aattattaac aatatggggt ttgataataa
    16981 gaatgataat gatatagata aaatatgtaa aaatctacta agtttagaat tgaatgaaga
    17041 aattgtagaa tctataagaa ttgatttgca agaaatgaag ttcaatggaa tcgatacaag
    17101 taaaaattta gtagaaattt taaagaaaaa gattaaaata gaaaatcaag caataaatgg
    17161 taaaattgct ctagtcggac caccaggagt aggtaaaacg actacaatag caaagttagc
    17221 cgcaaaatta gtttttgaag aaaataaaaa agtaggagta ataactatag atacatacag
    17281 gattggtgct gtagaacagt tgaagatata tactgatata atgaatattc cctttaaagg
    17341 ggtaataagt cctgatgaaa tggagttggc acttgacgag atgaaagatt gtgatgttgt
    17401 attaatagat acaacaggta gaggctacaa aaactctatg caaatactag aaatcaaaaa
    17461 tcttatagat aaagctgaaa ctgataatat acatttagtt ttgaattgta caacaagaga
    17521 aagtgataca aaagcaatta tagattcata tagaaatgtg aattttaaaa gcttaataat
    17581 tacaaagcta gatgaaacaa taacatatgg ctcaatattt aacataatga attatgcaca
    17641 aaaaccaata tcatatatta caacaggaca aaatgttcct gatgatatta taaaacctaa
    17701 tgaagataag attataaggt tactattagg agttgaaagt atatgataga ccaagctcaa
    17761 attttgagaa aaatggcaat ggaaaaaaga ggacttgatg agtttatagt agagaatgag
    17821 aatgtaccta agataattac aatagcttct ggtaaaggcg gtgttggaaa gagtaatctc
    17881 gctacaaatt tgtctatatg cttgaccaaa cttaataaaa aagttcttat attagatgca
    17941 gacataggaa tgtctaatat agatataata atgggggtaa atgtaaaagg gactataatt
    18001 gatgtaatta atggcgaaaa aaatatagaa gatataattt cacagactaa gtatggtgta
    18061 aatataatat ctggaggttc tgcccttaat catatagaag actttacaga agctcaaaga
    18121 aataagttta tacatagcat agaacaaatt cacgatgtgg attttataat aattgataca
    18181 ggagctggta tgagcaaaag cctattgtca tttatatact gtagtactga atttttctta
    18241 ataacaactc cagaaccgac atcattgact gacgcatata gtttactaaa agctataagt
    18301 aattttggca taaagagaag tgcaaatata ataatcaata gggtaattga tttagaagaa
    18361 gcaaaatcta catacaaaag aataaaaatg gttgtggaca aatttctaaa ccttaatcta
    18421 gaattgtatg gatatataat agatgacaaa aaagtaagtt tatgtgtaaa aaaacagata
    18481 ccattcattt tagaatatcc tacaagtatt gcatcaaaat gtattatagc catagcaaag
    18541 agaatggtaa gtcagtcaag agaagatatt gttgataata aaagttttct aagaaagatg
    18601 tttagtatat ttaaaagcta aggaggcgta gttaatgaat agagaagaat taataaaaga
    18661 gaatatgcct cttgtaaaga gtatagcaag aaaatttttt ataccaggga aaggttttga
    18721 atatcaagac ttagtaaata gtggagtcat tggattgatt gatgcaataa ataaatttga
    18781 tgctgaaaaa ggggcaaagt tttctagtta ctcttatata aaaataaaat ctgctatatt
    18841 agatgaaatt agaaatcaaa gtccaatttc taaacataat ctaactaaag taaataagta
    18901 taatagagtg gtagaaaagt tgcaatctaa attattaaga gagccaactt catatgaaat
    18961 tgcaaaagaa ttaaaggttt cagaaaaaga acttcatgat atcgaaagta atatagatat
    19021 gttaaatatt gtatcattaa actatgtaat ctttgaagat acaaatgaaa ctgtacaaga
    19081 tgtaataagt gatagagaag aggaagctcc agaaaatata atagaagaag aagaaaagtt
    19141 agagattcta tccaaagcaa ttagtaattt aaatgaaaga gaaaaattaa tactttcact
    19201 gtactattat gaagatttaa atctaaagga gatagggaaa gtgctaggtg tttcagaatc
    19261 aagagtttca caactccaca gaaaatcaat aagaaactta agaaacaaga taaaagaact
    19321 taaatattct atatagatgg tgattgataa gatgatacaa attatattaa tagtagtgtc
    19381 tattttaata atagtaacta tagttataaa tttacataag gaatcaaaaa ataaagataa
    19441 cagatacttt gataaaatat ttgagcaaga atatgaagca aatgatagat taataattgc
    19501 agaaaaaaga agattctttt tagacaaagt acttgcagaa aaaagaatta atagtcatat
    19561 aaaagtttat gaaaattata acctaataaa ggctaatgaa gaaagaaagg aaaacatatt
    19621 tatagaatca aaaaaggaag taagacaaga aaatgagtta tctcttaaag tgaaacatct
    19681 tataagttca ggttatgatg atgtggaaat atgtaagatg ctagatattg gaaaggggga
    19741 actgttatta ataaggagtt gttacaaaat ataaggatat tattatgtga taaaaaagta
    19801 ttcttaggaa taggtattgg tatattaata tcaactttat gtataatgct ttttagtaat
    19861 aatgatatga gtaaggcaga gatagaaaaa aaggccagag cgttaggcat gaaatataca
    19921 agtgagatga aagttttaga atagatgata ttgtttagac atttacaaaa aaggaggtca
    19981 taatgtgata agaggattat atacagcagt ttctgctatg ataactaatc aacaaaagca
    20041 aaatgttgtt gtcaataacc tagctaatat ggatacaaat ggatataaaa gtaaacaact
    20101 tttgactaaa agttttgatg aattaaagtt ggagggttac aataactacg caaatggaac
    20161 taaacaaaag cagattatag gcgatataag tccaggtatt tctatggatg aaactatcac
    20221 aaattttaat caaggaccta taaaaactac tgataataaa atggatgtag ctattcaagg
    20281 taagggattt tttctagtta gtgatgcagc tggaaatcaa ttttacacta gaaatgggaa
    20341 cttcagaaca gacaatcaag gtgaccttat aacaagtgaa gggtattatg tacttgggac
    20401 gaatactgct actggtgctg taggtcatat aaatgtagga aatcaaaaat ttgaagtggc
    20461 aaaagataat acaatatcac tagacggaaa tgctatgtat aaatttaata ttgtagattt
    20521 taataactac aataacttaa aaaaagaagg agataactta tactctggag gaggagctat
    20581 gaaagtaaat aatgcactta caatacaaag tgcgatagag tcctctaatg tagatatggt
    20641 atctgaaatt aataatatga tgacaatatc aagagaatat gaggcaaatc aaaagataat
    20701 tcaggctatg gattctaaat tagcaaaaat agcaagtgag attggttcag taagataggt
    20761 aataggaggt gaatttttat gtacacaatg ttttatacta gtaaaacagc tttatcagca
    20821 aatcaaagta aactagatat aatatctaac aatatagcaa attctgattc aacaggatat
    20881 aaaaaaataa atatggaata ttctgattta gtggatgaag tactaaatag accatcatac
    20941 cctacaaatg gtaaagatat atcgactgga acaggagcta aggcaacagc tcccatgaga
    21001 aattatgaac aaggtgcatt ggctcctaca gatagtaaga gtaatttggc gatagatgga
    21061 gaaggattct ttagagtgat aagaaaagat ggtacatatg catatacaag aaatggtgga
    21121 tttaatgtag atgctttagg taagattgta gatgataatg gaaatatatt agatgtccaa
    21181 tttgatgcag gatataacta taataataca aatatagata gtacaaacct gacagtttca
    21241 agaaatggag agctattcgt agataataaa aaaataggaa aaattaactt atatcaacct
    21301 ataggaactc aaaattttat atctgaagga gatagtttat ttgtcgcaac tgatgcagca
    21361 cagattaaac aagttgagaa agtcgatata gttcaaggct atagagagag gtcaaatgtt
    21421 tctttacaag aagaatttgt tgacttaata gccactcaaa gagcatttca aatgaactca
    21481 aagggaataa agacagcaga tgaaatgtgg caaatagcga ataatttaag agctaaataa
    21541 ttatgtaagg tgaggaatat tcatgaatga tatgaaagag tacgattttg gcaaacctca
    21601 gagttattca agagaaaatt taacttgttt aaatttttta ttagctgaat tttgtaaaaa
    21661 atataaaaac tatataattt atgaacttaa gtgtaattct agtatggttg ttgatagtat
    21721 agaccaagta aattatcaga cttttttaga tagagtaact tcttcgtgcg tagtagtaca
    21781 aaatgcaatg gaacctgtta tgaaaaactt ttcttttaga atagatagaa gtgtagctga
    21841 tatgtggatt gatataactt ctggaggaac tggagttgta aaggataatg atagagagct
    21901 tacagagctt gataaaaaag tgcttttaca ccttatggat gatttagtaa gaaatatgca
    21961 cttatttgag ggttttgaaa atatacaagt attggataca catacacata ttaatttgcc
    22021 acaattatgt tctccaactg caccagtatg cgttgtagat ttaaaagtgc taaatgataa
    22081 tgagtatatt ggaagtgttt ctctttgctt tccatacaat ggtttagagg ctctgttaga
    22141 aagtatatct gttatagaat tttttgataa tgatgtagga gatgattcag aggaatttac
    22201 ccaaaaaatc tataatagtg tatctaatat agaattatca gttattgcag agataggaaa
    22261 agtatcaatt aatgttgagg atttacttaa actagaagtt ggagatgtaa tagtaacaaa
    22321 taaaaaaata aatgattata tagatatatt tgtagaaaat tcaaaatcat acacagcaac
    22381 accaggattt atatcaagta aaaaaggtgt aaaaataaat gatgctgttg gaaaagaggt
    22441 gtagttatgg acaatagtaa tcttatttca gatgaagaag caaagatgtt attaaatgat
    22501 aaagttgaaa gtgaagttga tggagatgat gagttagcct tagatgtctc tgaagttaat
    22561 cgaaataata tacaaaggat acttgacctt aagttagaat tatctgtagt tataggaaga
    22621 actaagatga ctctaagaga tattttaaat ttacataaag gttctcttat tgaactggat
    22681 actttagcag accaagatgt ggaaatacta atagataata aggttttggc ttatggaaaa
    22741 gtagtggtgg tagatttaaa ttttggtgta aagataacaa gtatagtaag tgaagaagac
    22801 atggtaaaat ctcttgttta gagataacat caattaatac ttaaggcatg aggtggtact
    22861 tattttaaac ttaaatgata taaaaaaaga tatactaaaa ctcaataaaa aagactcatg
    22921 ttctctatat aatgaatcta ttaataatat aaataataat tgtatatcaa ctggattaaa
    22981 atttttaaaa caagcaagag atttaaaccc agatgatgtt gatatattaa atctcacagg
    23041 tcttgtaaac ttactaaaat gtaattttga tgatgctgta gaaagtttct ataagagttc
    23101 actttgtgaa aaaactgctc tgtgtgaaaa gtatataaac atgcttacat ctgaggagtt
    23161 tttaattttc tttgaaaagt acaatcaact tataaggtta ataaataaag gaatgtataa
    23221 agaagctata caagggttta aactcataac tgaccaatac tgtgatttaa tagaacctta
    23281 tgagttactt gccttactgt atgacaagga agaagaatat ataaaatttg atgaatgttt
    23341 agaagtattg aagattattg ataaggaaaa ttatttatta aatcaagaaa ataacacatt
    23401 agatatgtat aatagtgcta atatacaaac taaaagtaat ataaaagtag atgattataa
    23461 gaatgagttt ttacataaaa gtagtagtaa tcataaaatt aatgattata ataatgtaac
    23521 taaagaaaag agttcaagca aaaaccagaa aagttcagat tctaataaga aaaaaagtat
    23581 taaaaaaaat aagtacctat atatagccat aatattagga atattattag taggacaaag
    23641 tatatatagt tcttataaga tgaataattt aactgataaa atatctaaaa ataatgaaac
    23701 taaaaatgaa gagtcaatag attctaactt gactaagaaa gaaaataata tagaagaaaa
    23761 tgaagatatg agtaaggctt ctaatataaa taagagtaat aaaaacatta ataaagagga
    23821 tgatgtaaaa tctaaaaaaa atactgtaaa aaatgaagat gataaagatt ctgacttatt
    23881 tacagaggat gaattgatgt ctaaggcgaa tactttaaaa tcagaaaaga aaactaaatc
    23941 ttctatacaa ctatataaga aagttgcaga tataggtgaa aataaaggga atacatctga
    24001 agcaacttat caagttgcta ttctcagtga aaagttaaaa gactataaaa ctgctgaaga
    24061 atattataaa atgtatgtag aaaattactc agaaaaagat gcttattttg atgaatctta
    24121 ttataattta ggtatgatgt actataacaa tggtgattta aaaaattcta aattgacatt
    24181 aaagaagttg gttaataagg taccaaatag tatgtataat aattcaaaag taaaagaaat
    24241 tttaaaagaa gaatagttaa attagataag aaaaataaat t
//
LOCUS       DAEORZ010000049        23372 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEORZ010000049 DAEORZ010000000
VERSION     DAEORZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.049"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(543..1184)
                     /locus_tag="KRH39_001802"
     CDS             complement(543..1184)
                     /locus_tag="KRH39_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG8511712.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQVSDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            complement(1427..2230)
                     /locus_tag="KRH39_001803"
     CDS             complement(1427..2230)
                     /locus_tag="KRH39_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG8511713.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDVHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            complement(2386..3561)
                     /gene="thiI"
                     /locus_tag="KRH39_001804"
     CDS             complement(2386..3561)
                     /gene="thiI"
                     /locus_tag="KRH39_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBG8511714.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            complement(3607..4758)
                     /locus_tag="KRH39_001805"
     CDS             complement(3607..4758)
                     /locus_tag="KRH39_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBG8511715.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            complement(5406..6128)
                     /locus_tag="KRH39_001806"
     CDS             complement(5406..6128)
                     /locus_tag="KRH39_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG8511716.1"
                     /translation="MITIKNLSKSFGDLNVLNNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(6187..6864)
                     /locus_tag="KRH39_001807"
     CDS             complement(6187..6864)
                     /locus_tag="KRH39_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG8511717.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(6892..7707)
                     /locus_tag="KRH39_001808"
     CDS             complement(6892..7707)
                     /locus_tag="KRH39_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG8511718.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(7933..8143)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8736..10802)
                     /locus_tag="KRH39_001809"
     CDS             complement(8736..10802)
                     /locus_tag="KRH39_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG8511719.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(10879..13908)
                     /locus_tag="KRH39_001810"
     CDS             complement(10879..13908)
                     /locus_tag="KRH39_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8511720.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRAGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSDDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(13961..15421)
                     /locus_tag="KRH39_001811"
     CDS             complement(13961..15421)
                     /locus_tag="KRH39_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBG8511721.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIDSSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFEIFKAELENAIKK"
     gene            complement(15513..15887)
                     /locus_tag="KRH39_001812"
     CDS             complement(15513..15887)
                     /locus_tag="KRH39_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511722.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKVNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(16063..16803)
                     /locus_tag="KRH39_001813"
     CDS             complement(16063..16803)
                     /locus_tag="KRH39_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG8511723.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(16824..17612)
                     /locus_tag="KRH39_001814"
     CDS             complement(16824..17612)
                     /locus_tag="KRH39_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBG8511724.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(17700..17790)
                     /locus_tag="KRH39_001815"
     tRNA            complement(17700..17790)
                     /locus_tag="KRH39_001815"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17754..17756),aa:Ser,seq:gct)
     gene            18049..18813
                     /locus_tag="KRH39_001816"
     CDS             18049..18813
                     /locus_tag="KRH39_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG8511725.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(19094..20209)
                     /locus_tag="KRH39_001817"
     CDS             complement(19094..20209)
                     /locus_tag="KRH39_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG8511726.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(20250..21776)
                     /gene="glpK"
                     /locus_tag="KRH39_001818"
     CDS             complement(20250..21776)
                     /gene="glpK"
                     /locus_tag="KRH39_001818"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG8511727.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            22069..23166
                     /locus_tag="KRH39_001819"
     CDS             22069..23166
                     /locus_tag="KRH39_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG8511728.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
BASE COUNT         7504 a         4201 c         2423 g         9244 t
ORIGIN      
        1 aaaatttaga tatttttcta aaaattatct atgttaatag ttaaatttat gaacattttt
       61 tgtttttatg ttaggatatt tcttagaaat gaaatacttc ttaatttgat ttgatatgta
      121 tatttttata gctattttat tataaaaaaa aagaaccctt ttataactaa gggttacttt
      181 ttttctctat tttttcttat tatatcacta ttttttccct atttcaatat tttgacattt
      241 tttccattat actgaaaaga tatcttaaat ttttaacttg gataaatttt aaagcatata
      301 cttaaaatta acatacttgt tttctatttt attacactta atccttattt ttatcattct
      361 acaaaacaaa aataaaaaag aataagataa taaataaaat acttatcacc ttattcttta
      421 tttaattatt ttacaacgaa aaatctaaac tactttttaa atagcttcct cttccatcaa
      481 tttatttgta tattactggt ttaattcttc aagtaactga ttggttcttg caatacttta
      541 tcctaaactc tggaaacttt gtaaactacc tctctagcat ttggtaaatt tccatctgga
      601 atacctacag caataccaaa tggtacagaa tacccttctg gtatatcaag tagttctctg
      661 tattttttat ttgtatcaaa cttcatcttt attgactctg attcttttac atcttcttta
      721 tccaatacac tcacaagacc ttcaatatcc ccttgttcaa caggtgtaaa taaacctcta
      781 accattccta ttatacaact tccaagccct agtgcttgtg cagctgttgt tatattccct
      841 gtaagtattc ctatatcata tggagcccat gaactacttt catcataaga aataaataaa
      901 actgttggag caccataaaa taacttaaag tttttattta tctcaacatt actcacctca
      961 tgaatcctag ataaagtatc atcactaatc tcttttaaaa gtttctggtc ttgcacaaca
     1021 gttatgtgcc aaggctgttt attacatcct gaaggtgctg caaatgcaca gtgtaataat
     1081 gtgtttactt gctcatcact aacttgttct gtcgtaaatt ttcgtatact tctacgactt
     1141 tgtattaaat ttattgtttg gttgtcttga aaattattat tcatatctat tccccttttt
     1201 atgtatttct tagtacaaat actaaatctt cagaaaatta atcttatagt taaatttctt
     1261 tacaatatta ttataattat ttttagctta tatgtgaaga cttaaaaata taaacacttt
     1321 taatatatct caattatata tataaatgaa aatctggtat tcgtcatcta tagaaactgc
     1381 tcttttctat agtttaatca ataccagatt aataaaacat gatatattaa tttaaaggac
     1441 cccatccctc tttagaatga atctctgttt ttacagactt aacactatct gtgtcaacat
     1501 cccatgacct acctgcaaca gaaataccat ttttaccttt ataccatcta ccttcatcat
     1561 aatcgtaaac tagggaagta taccaattac ctccagtctt attatttgca aatacaacta
     1621 cagtagcatc agatttagta ggattatatg catatggttg tatatatagt gtatccttac
     1681 ttaatccata ttttgcctta aattcatctg gaattgtagg ccacccattt ttatatttat
     1741 tgtctaataa atattttcta aaatcatcat tgccctttga agctcctggt attatagaag
     1801 ggtcttgtgc aaatgatgca atcaaatctt tcatactttg atgcacatcc ctagccattt
     1861 caacaccgtc tggatgtttt gtacaagtaa catacacttt tgcaatgctt tctattccag
     1921 ttattccatc ataaccatca tcaaacgttg ctgaatatat tccacctgac ttacactttg
     1981 gttctgtttc aaagtactta ccatctttgt tttctaatac ctcttttatg acttcattct
     2041 tgtctttact tgattcctca gccattgcaa taacaatttg agtctttata ttttctctat
     2101 tagaaagaca tgtaactgct ttactttttt ctatatttct aaataacgct ggaacagcta
     2161 ctataactaa aattcctatt atagatataa ctaccaacaa ttcaattaat gtaaaaccct
     2221 ttttattcat aataatcatt cccattaaat aaaattatag tacttatttt taaatactac
     2281 atattaataa attgtctaca taaatccttt caacagaact actaatatac ttacagaact
     2341 actaatatac tcaatattca atttagtaat taaataactc gtatgctaaa actcaatatc
     2401 ctctacctct attttgtcaa tagcatcctg tactaatttt tcaatgtcaa gtgctgtttc
     2461 agatttttct attttctcta atctaggttt agtgacgggc tttctaggag aaaatacact
     2521 acaacaatct tcttcaggca ctatagatgt ttcaaaagtc cctatttttt tggctatatc
     2581 aactatatct gatttatcca ttgctataag tggtctaaac actggtaagt ctacaacggc
     2641 attagtacaa gtaagccctt gtatagtttg agatgcaacc tgcccaatac tttcacctgt
     2701 aattaaagca tcacaatgtc ttttttcaga aagtctctgt gctatcctca tcataaatct
     2761 tcttgaaaga atagtagctt cttcttcatt acaattctcg ccaattgctt tttgtatttc
     2821 taagatatta actttatgaa gccttactct accacagtat tttgccagta ttttagctaa
     2881 atccttaact ttttcctgtg acctttcact tgtaaatgga taactatgaa agtgaactgc
     2941 ctctacttcc atacctcttt ttgcaaccat ccatgtagct actgggctat ctatacctcc
     3001 tgataaaaga gacatagctc taccattcgt tccaagtggc aatccaccat atccaggtat
     3061 agtatcacta tagaccatag tatgaaattc tctgtattcg cactttattt taacttctgg
     3121 atttcttaca tcaacattaa tcctatctcc tacctttgat aataagtatc cacctatatc
     3181 catactcatt tcttgagaag tcaatccgaa agacttatct cctcttcttg actctacctt
     3241 aaaagtttta tatccagcct ctattttttc ttctaacatt tttaaagcta tttcttttaa
     3301 tgtatcataa tcttttttag ctcttactcc agggcacaca cccactatcc caaatacttt
     3361 tctaacttct tcaattactt cttcataatc atagtcttct aaatcaacat atattcttcc
     3421 atactcttta tatacattaa atcttccaat tggttttaac atatttctta tatttcttat
     3481 aagcctattc tcaaatatat atctattctt tccttttact ccaatttctc catactttac
     3541 tattaatata ttatacacca tttcacctct taaactaatt tattatcact atttatacag
     3601 tattctttat tttcttttca ttattgacct taagtcacaa attgattctt tcaaaacttt
     3661 cacagcttct aacatttctt cttctgtatt catatcagat aaactaaatc ttattgttcc
     3721 atttatttct tcatttgtaa gacctatagc atttaatacg taactacctt tctttttaga
     3781 agaacatgca gagccagttg atacataaat acctttttgc tctaagtaat gaagtaaaac
     3841 ttctcctttt gtccctctaa atgatacatt taatatatga catactccat cttctggaga
     3901 attgattttt atatcttcta tattatcaat tatttcattt tttaatatat tttttaagtt
     3961 atctactttc gaaataacag catctaaatc tttgtttagt attctaacag cttctccaag
     4021 accatatatt ccaggtacat tttctgttcc cgacctaatt ccaatttcct gaccaccacc
     4081 tgttaacata ggcttaaggc gattattttc ttttatatac ataaatccaa ttccctttgg
     4141 accatgaaat ttatgtgcac ttacactcat aaaatctata ttgtatttag atggtctaaa
     4201 gttaattttt ccatatgact gaatagcatc aacatggaaa taaatttttt cttttaaacc
     4261 cttcaaatac tttccaactt cacctattgg ctgtatagtt cctatttcat tgttaacatg
     4321 catcatgctt actaagcatg tagttgactt tatcgcattt tttaaatcct ctatatttat
     4381 tttcccatct tttccgacct ctaaataagt tacttcaaaa ccatcttctt ctaaatcttt
     4441 taatgtattt aaaacagaag gatgttctat gttagtagat attatgtgat tttttctttt
     4501 tttatttaaa ctagtaatac ctcttattat agtattatta cactctgtac cacctgatgt
     4561 aaaataaatc tccttttctt tcgctcctag agatttagct atatcttgtc ttacttcttt
     4621 tatagctttt tcaacttcta caccttttct atggacagaa gaaggatttc catattccgt
     4681 attaagtgca taaaccattt tatctataac ttcttgataa ggttttgtag ttgcgctatt
     4741 gtctaagtat atttccaact taattacacc tacctttatt taaaatcttc aactctaact
     4801 attataccac taattatagt attttttttg ttaatcttta ttaaaaagac aatcttaaat
     4861 tataaaatat tatttcatga aaagctagtt ttttcacttt attacaaatt tttatagtaa
     4921 aatataatat acttatacta taccctacaa aaatatatta taaaatacaa tatatgataa
     4981 aataatatat tatataaaat aaatatatta tattatacat atacttattt ttactataaa
     5041 agttaaattt ataagcttac ttgttataaa aataactttt ataatttatt tcaaaaaaaa
     5101 tattgatttt tgtgtcgcat tttgttataa taaattaagg ttatccccct ggtaacccta
     5161 gttagttaat atatctattc ccaataccct ttcataagga tatattttat atttattaaa
     5221 aatcggatta gtaacggaaa ttaattcggt ggaaaaaaag agcttacccc gagctctttt
     5281 tttttctgca aaaaatcgta aaaataaagt taatattaat ataactttaa ttttaaaatt
     5341 aatattaacc atcactaaaa aaggatgtat tgaatttcta atatcaataa catccttttt
     5401 atatattata gcacttttgc taaaaacgct tttgttcttt catgttttgg atttccgaaa
     5461 acttcttctg gagtattatc ttcaagtata cttcctccat ctataaaaat aactctatct
     5521 gcaacttcct tagcaaatcc catctcatga gttacaattg ccattgtcat accttcttta
     5581 gcaagttctt ttataacatc caaaacttcg tttaccattt ctgggtcaag agcagatgtt
     5641 ggctcatcaa acaacatcat atctggttcc atagcaagtg ctctagctat tgctattctt
     5701 tgcttttgtc ctccagataa ttgtgatgga tatgaatctt ttttatctaa aagaccaact
     5761 ctactcaaca aaatttctgc ttttttttct gcttcttcct tagtcatctt ttttaattta
     5821 attggtgcta atgttatatt atctaaaata gtcttatgtg ggaataaatt aaagttctga
     5881 aacaccatac ctatattttg tctaacctca tcaatattag tttttttatc gaccaaattt
     5941 tttccttcaa atattatttc tccacctgta ggtatttcta gtaaattcat acatctaaga
     6001 agtgtacttt taccagaacc acttggacct actataacca ttatttcgcc tttagcgatt
     6061 tctaagtcta tattatttaa tacatttaaa tctccaaaag atttacttaa attttttatt
     6121 gtaatcatat tatcactttc ctttttacta ttctatgttt tccctaaatg ctaaacatgc
     6181 tctcatctag ttagacacag atagtttctt ttctaataat ccaactaatc ttgatagagt
     6241 aaatgttata acaaagtaaa ttgctgctgc tactattaat ggctctaatg ctctaaatgc
     6301 tgcatttttt actatatcag cagtacgcat tatctcaaca actcctatta cagatattat
     6361 tgaagattct tttacaagtg taataaactc atttgctaat gaaggtaata tattttttat
     6421 agcttgaggt attattatat atctcatagt tgaccaataa tttaatccta atgatctact
     6481 tgcttccatt tgtccattat caactgattg tatacctgat ctaagtatct ctgccacgta
     6541 tgcaccagag tttactgata atgcaaatgc actagctata aattcacttg gtataccaaa
     6601 caacataggg aatcttattc ctaattgagg taaaccaaac catactatat atatctgtac
     6661 taataatggt gtccctctta aaacctcaat atagattgat gatattgctc ttaatacttt
     6721 gctctttgat atttttgcca tacagataat tatacctatt ataaatccaa agcataatgc
     6781 cacaagagaa attgatactg tcacacctgt tccttccaaa aaagaagtcc caaatctact
     6841 taaaaaacta aaatctaaac tcataatttc atctcccaca taactttctt actaatttga
     6901 agcagcttca gtagatgctt catttaaaaa ttcttctatc ttcttgccat cttgtaattc
     6961 ttttatcttt ttgtttaata attctataaa atctttgtta ttgtcagatt ttttgattgc
     7021 tgctgccata ccttctgtta catctttccc aacttcagta tttgccattt ttattccatc
     7081 atatttttta acatttatta aagaaactgc ttcattagta acaactgcat ctatgtttcc
     7141 attttttaat tctgtaatta aatcaggtat tgctttcaaa gattttgtag ttgtcatttt
     7201 taaagtgtta actacatatt cttcttggat acttcctgct tgaactccaa ctctaagttt
     7261 ctttaaatca tcttcagttt ttactttgtc tatatctgca tctttaacta taactgcatt
     7321 tctacttaaa taatataatt ctgaaaaatc aacactcttt tctctctctg gtgttggaga
     7381 catacctgca agaacaatat caacttggtc tgcatttaaa gcacctatta atccatcaaa
     7441 tgacatatcc ttaatttcaa cctttacacc aagctctttt gcaaattctt cagccaacat
     7501 tatatcgaat ccttttattg aatcttttcc atctactacc ttatgaaatt caaatggagg
     7561 aaattctgca ctagtaccaa caactaattt tccattgtct tttatttgtt ctaactttgt
     7621 tttttctcct ccaccactac atcctactag cgataatgtc aatcctaata ctaatcctaa
     7681 acttaataat tttttaaata ctttcatttt aactacccct ctcttttaat tataatttcc
     7741 ctcagatttt aatacttaaa tgtttcgagt tacaaaaaaa gtctctcagt ctttaatgac
     7801 taagagacga ctttaccgcg ttaccactct tattgataaa tacttatatt actttcaaaa
     7861 tttgtgactg ttagctttta agctgtatgg atatttttat tttaaaatca aaaaagtccc
     7921 ttagctttac agctaagaga cgaattatat ccgcgttacc actctttttg ataagtaagt
     7981 acttatcctc tcaatccctt aaggcgggta aaccaattat catactaaag tttctgataa
     8041 tctcctccag agctctcttc acatagactt cattgctagg cttacaccaa cccctaactc
     8101 gctgtaaatc catatatgct actttctcta tcattggatt tgtttcctta aattgttaat
     8161 acaattgtac ttccttataa tatttttgtc aataagtttt ttattttttt aataattttt
     8221 acttgtaaat tatctaaata ctactgttta tatttattga tattatttgt gtaattataa
     8281 atagtatatt tcatattttt agctatttct taaataatac taaataatat ctttcacatt
     8341 gttgcttttt cttaaacaat actaaataat atctttcaca ttgttgcttt tcttaaatta
     8401 cactaaataa tatctttcac actgttgctt tttctttaat aatactaaat agtatctttc
     8461 acactgttgc cctttcttta ataatactaa ataatatctt tcacattgtt gctttttctt
     8521 aaattacact aaataatatc tttcacattg ttgcttttct ttaataatac taaataatat
     8581 tttccacatt gttgcttttt ctaaacaact ataaataata tattaaaccc ttatttcttt
     8641 tttctaataa gttaaataat acattttaca catatctatt tgtcttgcac ataataaata
     8701 atacattata tttctatcta aatttagatt ctaaattaaa tagtataaat tatattcttc
     8761 aataaacaag ttttcaaatc caagattcgc tctctatctt taaactttat tgtaaacata
     8821 attccatctt ttttaattat ttctccttca tgaaattttt tatggtatac cttatcccct
     8881 acaccaatac tgtcaatttc ttttttcgtt ggagatttta tatcattaat aaataatgat
     8941 ttatcaactt tcttaccata cttatttaaa gtagagctta tacataactt ttcttcagct
     9001 cttgtaatag caacatacat aagtcttctt tcttcttcta attgttcttc ttttttctct
     9061 tccttacaaa tatcatatga tttttcatga ggtattgttc cttcatttac acctataata
     9121 taaacatttc taaattctaa tccttttgca ctatgcatag ttgtaaatat gacaccctca
     9181 gtctgcttat tcttgttatt ttccgttaac tcagtcttta ctctatcaat atgctctaaa
     9241 aactctgtaa cagtattaaa attagttgct gaactctcaa gttcatttaa tatctctaca
     9301 agtccattag tctttatctt tctgttgcta caataatcta aaacatatct atcataatca
     9361 agactagttc taatataaga tattgcattt ttaggattta aagtatttaa ataacttaaa
     9421 tctatttcca aatcatttat tgttttaact tgtttaggat gaagattaca cttatttatt
     9481 aatgctgtta taaagtcttt ttcatcctta accatattta tactgtcttt ggatatatat
     9541 ctaaaaggct tgtttataat tctcaaccaa tctttatttg attttggatt aatacctatt
     9601 cttaaatagg ctagtatatc ttgacttgcc caatggtcat atattgtaat tactgagtct
     9661 tttactacaa atggaatcct catatccata aatacatcta ctaatgctct tgactgtata
     9721 ttagttctat aaattactgc aaaatctgaa tactctacat aatctttttg tatttcgtca
     9781 attatttccc tggcaatata taaggcttct tcctctgaat catgcggaga aatataggta
     9841 acacttcctc catccccctg actacactta atgatttttt catatctatt tttatttttg
     9901 tcgataagtc tatttgcagt atgcactatc tcacttttag acctgtaatt tatatctaat
     9961 attatttttt tagtattatt aaagtattgt tcaaattcaa gcaaaaaatc tggtctagca
    10021 cctctaaatc catatatact ttggtcttca tctccaactg caaatatatt gtttaatggg
    10081 ctacaaatta actttaaaac ttcaaactgt actttattta tatcttgaaa ttcatctatc
    10141 aatatatatt tatacacatt tctaaccatt tctagtgcag acttattgtt caaaagtaaa
    10201 taataagttc ttataagcat atcatcaaaa tctattttat taactttaga tttttgttct
    10261 tcgtataagt tgtatacttt taaaaactca tcttttgtta atacttctga attaaattca
    10321 tttttatcca taagttcatt ttttacatag gaaatttcat ttataacttg tcctatattc
    10381 tcatcatcat cagcattctc tatatttaaa ctttttagga ttgccttgat tgtcattcgc
    10441 tttgactttt catcaaatat actgtctaag ttgtatcttt caaagtatct tagaatttta
    10501 aaaaatacag agtgaaaagt accataggtt accttattaa gtcttatatc atcacttaaa
    10561 ttcaaagctc tatttttcat ttctattgaa gatgccttgg taaaacttat tgccaagatt
    10621 cttgttggtg ctatgttttt gtttacaacc atattggcta ttctatatgt aattactctg
    10681 gtcttacctg aaccaggccc tgcaagtatc atgcaaggcc catctatatg ctcaactgct
    10741 tctaattgat tttcatttaa ctttcttata tctatatttg ttgtgattat tagctccacc
    10801 accttgattt ataattatat tattttcttt ttaattataa actaaaagtt cataaaaata
    10861 aatttaattt ttcctctatt aaaccacatc attcctattt tcatgacata tcttgagttc
    10921 ttttttaatt ttgtcgctta tatcttcagg tataaatgat ggtttactaa tatagtagcc
    10981 ttgcatataa tctactccaa gattaattag tgtttccatt tcaccttttg tttctacacc
    11041 ttctgctatc actttaatat ttctttcgtt agaatatgag ataagatttt ttaatatttt
    11101 ctgtctgttt tcatctttat caatacctct tacaatagac atatcaatct ttacatagtc
    11161 tggcattata tataatagta cagcatctcc gttgtaacct gtcccaaagt catctaaagc
    11221 aatttctata ttccaatggt ctatatatgc tttctttttc tcaataaaat cttcattaat
    11281 cttatcattc tctgttattt ctagtactac tctatttaaa tattcaccat ataaatcttc
    11341 aatttcctca aaatctactt ttgacaaatt attattagga atagaattta agaacaattt
    11401 gcaatcgcca aatttttctt tgtatttttt aaatgattta agtgctttaa accatgtaat
    11461 acgctcaatt tgatataatt tagactgtga acgagctaat ctaatgacat ctattggcga
    11521 actaaaagtt tttattttag gtctcataag tgcttcatat gcaaatactt tacctttaca
    11581 atctacaata ggctgaaatg catactcaac taattgacca tcaattaact tgtttaattc
    11641 ttctctatta ctcaataaga atgaatcctt attatatata ttaatatcaa attcactaac
    11701 ttcacccttg attgtatttt taatttgata catagcaaaa tctgcgtatc tcattaattc
    11761 atcatagttg gttgaatcat cagggtacca ggaaattcct gctgatgccc ttattttaaa
    11821 ttctgttcca tcatgaagtt ttaataaggt attattcatc tgtttcttaa cagaattaat
    11881 aatatctctt atactatctt tactttcata tccatatata aatacataaa actcatcatc
    11941 tgaaagtcta gatactatac cattccatga tataaacttc ttaagtatgt ctgcagcaca
    12001 cctaatatat tcatcaccag catcatgtcc atatgtatca ttaatatatt ttagattatc
    12061 taaatcccac ataataaatg ctgctatctt aagttcatct ttattcttaa atttttcttg
    12121 tatctgtgaa tgaaaagctc tcctatttaa aagattcgtt aaaatatcaa aatctctttc
    12181 atattctatc ttccttttct caataaattc ttttgttaca tcaacaacaa caccaagaac
    12241 ctttagctca tcttcaaaga tattcattct tatccactta agttttttat ctttagattc
    12301 aaacttatat atatatgtat tttctgtttc ataactttct tgcaaatatt tatctagtcc
    12361 ccttaatttt ttcaggaact tatttttttc catatatcca tattcttcaa atttgatttc
    12421 agtagtaaac acgctaaaaa attcttttgt acaaaaaaca ttctctgcgt ttttgtataa
    12481 ctcaaaagct ccaattggca tatctaccat ttcaataatc tgtgataatt ttgacgaaga
    12541 atctgcaaca tttgcactga gcaactctat agctgaagat aattcatcta tttcagatat
    12601 atttatctta tcaagattta ctggttttga tggattactg tttctaactt tgttggctaa
    12661 aagaacaatt ggttttgtaa ataatcttga tgcaatataa atacctatta gacctattaa
    12721 taaagaaatt gctatagata ttataactaa tttctctacc ctttttgaaa atgacaaaag
    12781 acttttttct tcaataatac ccaaaagagc ccaacgttca ttttcaaatg gagtattgga
    12841 gttatataaa tccaagtatt gtacactagc ataaatgttg ccattaccac attcattatc
    12901 tttattttta atcttatata tatttttata cgattccttc tgctctaaat tagaatgtaa
    12961 accatcccaa aatttttctt ttaatgagta tccatttgaa attacatttt caaaggccat
    13021 atcattattt ctatctacac ctaaaatata attacccttt ttattcccat ttatctctga
    13081 gtatggaagt aattctctta aataatttac acttaaatct attccaagta ctccatatac
    13141 agttccatct tcataaataa ggggaactgt atatgtcata agcctatcac ctgcatttgt
    13201 caataaaaac ttacttcccc agtatcctaa attagaactt gatacatttg gattatcaat
    13261 tgcagctcta aatggtttat aaaaaaaatc atctccatct tgctcatttt cacttttaaa
    13321 tgaaaactta ggttcccaat aactgtccat agatattcca aaacttcttg ctatatctga
    13381 tgaacctctc tcaattaaca aatcagaatt atcatttgga ttaaacttag ggtctaaatc
    13441 ccttatatac aatcctgccc tacttatttc acctttacta tctattgact tatcgttaat
    13501 atttttgtca cttaggatta taaaagctcc tgtaacagaa ttttttctca aaagatatat
    13561 taaatcattt gatatgcttt ttataatatc tttgttaatt tcttcattgt tgcctacatc
    13621 ctcaattaag gcattttttt catttaatac tttttttaca gaggaattaa ttgtagcttc
    13681 cgtttcactt gtatttgacc atctttgtaa catttcattt tgtatataat ttttacgatt
    13741 aattactctt tcatttaaga tgtcaaacga attatcattt aatttcttaa tagttcctcc
    13801 ccataaaatt gtggcacaaa ataaacttgt ttgaattagc ataacaataa ttaggggaaa
    13861 tatcatcttt ttcattattg aattgttttt tcttagattg ttttccatac ccattccacc
    13921 tatacctttg tgctcgtcaa cacactaact gccaattcat ctattttttt atagcatttt
    13981 caagctcagc cttaaaaatt tcaaaccaag ttttaaaatt tttatcatta ttaaattgtg
    14041 ctactgcatc cttcttagaa gttccacttt ttactaattc atttatttta tttgaatctt
    14101 ctttagcttt atcaagcatt gaatattcaa gtatttgtct agctttagtt ccaccatcaa
    14161 atgctttatt agtatataaa ttacaactgt taatctgttc aatagatgag tctattgaac
    14221 cttcaacttt atcagataaa ctcaaattat ctttcttcat aacttctttt ataacatcca
    14281 aatcattcgt ttttttcttt actggtaagt atccagaagc aatagagaat tttatattag
    14341 gttctttatc tgtaaaccat tttaaaaatt ctactgatgc atattcttct tctttagtag
    14401 attttgttac agccattcct gcaccttgtt gtacagcata ttttccttct cctttaaaac
    14461 gtggtgcttg aaaaacagat gtttcaatat tataactctc attatcattt agcattactc
    14521 tatctggaaa gtatgctgct cctgaactag aacctacaag agctaatata tcacctgttt
    14581 ttgcatcatc agaacgaaac ttaccataag aagcaaaata tccatttata tatggtacat
    14641 agaaattatc ccatagtttt ttcataactt tttcatcaat atttagtgta actttaccat
    14701 ctttaacaga aaaaatttct actcctagtt gtttacttcc tataataata taatttgcta
    14761 aagcatctct tccaaaaaat gctttaccat cattaggctc tttcgttaaa ctatctgtcc
    14821 attcataata tttttttgat gtctctacta atccttcaat tgtttttata tcatttatat
    14881 ttgaatttgt agcttttgca aatttatccc aatcagtttt atttaacatc aaaatttcag
    14941 ttgattttgc aattgggaaa agtttaaact catccttaga atttattctg ccttcttcta
    15001 catattcgtc tatgtattca tttaattctt ctttacttat gtacttatcc atatctacta
    15061 gtaaccctaa tttatccatt tcatatgcag tatctgagta tgctgcaaat atatttggta
    15121 catcttcaga accaactttt ttatttgcag aatctaaaac tttctctgat aaatcaacta
    15181 cattaccttg actgaatgct tcaacaataa ttcctttttc agcacctaca gtgtcattaa
    15241 attccttaac aagcttatca aaagccattt tctgctgtcc attataataa tgccaaattt
    15301 gtattgatgt cggattttct gggtctaact tataattatc ttccttcttt aaactacatc
    15361 ctgtaagtaa taaaacaatc attgatgtga taattaattt tgcaaactta cacctattca
    15421 taaaatctac tccttcaaat tataaaattt ataatagcat taaaactgta acttcactat
    15481 ttatattaat tatagaacgt ttattgatat atttaaacag gatttacaac atatttaaaa
    15541 gcaattaaga aacatactat tacaaatata gaaaataaag cttttacata atttttattt
    15601 ctaaatgcgc caaatgacac caatccttgt tgaatagcta ctgtaattac taaaagtatc
    15661 attattatat taagttgaac cagtttaaat aagtaaagca atgaaaacac tactagaagt
    15721 acacataata caatactaga tttattaata atatcatcaa tcttatctac cttcttagat
    15781 tgcttattaa ctttatttaa tttttcttta tttaaattct caactttttt attatacctt
    15841 tctcttgttt tatctttata tttagtattt ttatttttat ttcccattta ttttccatcc
    15901 tctataaatt ttttaattga gcttatacat tatttaaata ttatatattt aagataccat
    15961 atattcctac aaaaaggtaa cttactatgt atctattttt gattttcgca taaaaaagcc
    16021 atctccttta gaattaattc tgtattgaga cagctaattt aactatttat cctttgaaac
    16081 attttctaca ttatcatatt tactatcaac taaaactata tcaactcttc tattttttga
    16141 cctaccttct tcagatgaat tttctgctat tggtctaaac tcaccatatc ctacagcaga
    16201 taatttatct ggtgctatac ctgcattgtc aataagaagt tgaacaacat tggttgctct
    16261 cataactgat aaatcccaat ttgacttaaa ctctgaattt tttatagaca tattgtctgt
    16321 atggccttct actctcatgt aactattcat ttcatttaac atcttaccta tttgaataat
    16381 cttatctcta gaattatcct tcacaatcgc tttccctgta tcaaatagta ttgtatcttt
    16441 aaggcttatt actaatcctc tatcttgaac tcttaatgat actgaattag ccataccatt
    16501 ttcgtttaaa tacttctgga tttcttcacc aactttttta aacttagcat tttgtaaagt
    16561 atctaaatca ttacttcctg gttcaactac tggttctatc ttgctattac caccttcaat
    16621 aactccagaa gaaccaccaa aagcagaatt tagagattga gataattgtt tatatttctc
    16681 cgcatcaaca ttactcatag aatacattat tacaaagaaa atcataagta atgttatgag
    16741 gtctgaataa gttaaaagcc atcgctcatt gttttcttct ttttcatctt catcatacaa
    16801 cataaacatt ccatcctttt atattattca gctctaccac caatttcttc aagttcaatt
    16861 agttcttttg aatttaaaaa tatttttaat ttttctgtca ttaaattaga attatcaccc
    16921 tcaagtatgg ataatattgc ttctatcacc aaaagtcttt cacttacttc ttgttgattt
    16981 atagccttaa gttttgatgc tattggtaac catattaaat tggcagaacc aacaccatac
    17041 aaagtagcca aaaatgcaac agaaatttga ggtcctaatg catctggatt acttgaaaga
    17101 ttacctaaaa tatgtactaa tcccataact gtacctatta tccccagtgt aggtgcatat
    17161 ccacctgctg aatcaaaaat tgctattcct tctttatgtc gttttgatgt tatctgtatc
    17221 tgtgattcca atatatttct tatagtaact gcatccactc catctaccat aagttcaagc
    17281 ccttttctaa taaaagggtc taattcatca ttctcactta tttctttgtc aatactaaga
    17341 agcccctctt tcctagcctt tgttgcaatt tctttgaaat agattatatt ttctatgtta
    17401 ttaggctttt tatttttaaa agctaataac attatcttta tagacttttt aaacacaggg
    17461 aatggagtcg aagctccaac agctcctata gttccaccaa acacaacaag tgctgatgaa
    17521 aatacaaata atgctccaac atgtccccca tcaataacaa aagcaactac caaagatgct
    17581 atccccacta tgaaaaaaat taaagttgtc atctattccc tcctttcttt agttatacat
    17641 atctaatcat ttatgacatt ataaaaagac tatctttaat aaaaatagcc tttaataatt
    17701 ggcggagaag gagggattcg aaccctcgca ccggttaccc agcctaatcc cttagcaggg
    17761 gatcctcttg agccacttga gtacttctcc atatctctat actagtaaat tatataataa
    17821 aatcatattt tagtcaattg ttttataaaa tttgagttat tttgaattat taaacatcta
    17881 tttttacttt tttgcttaaa tgtaagtttt aaatatggta aaattatctt gcataacact
    17941 tataatatta tttgataaat atacatacat taacaaatat acctaaactc tgacttaatt
    18001 gtaagttatg ccaaaactaa aataatacta gtccaaggag gatattttat gttaccacag
    18061 gaaagatatg agaagatttt aagtattctc aaatcagata atataattaa aataaatgac
    18121 atagttcaaa tgtttgatat atcaatagaa actgcacgaa gagatttagc ccatatggag
    18181 agtatcggtc ttttaaaaag agtttatggt ggtgctatac cagttatagc aagtagcgta
    18241 gaacttaact atgttaaaag aaaaaaaata aactcaaacg ataaaaaaca gattgctatt
    18301 aaatgtagtg agtttataaa tacaaatgat actattttta tggatactgg aactactgta
    18361 cttgaagttt caaaaaattt gaaagataaa aagaatctaa ctgtaataac caactcaatt
    18421 ttagttgcaa acgaacttat gaatactaat ataaaagtat atgtgcttgg gggctatctt
    18481 agagaaactg aaggttcttt ttctggtccg cttacactgt cagctattga acaatttaat
    18541 gttgataaag ctattttagg cgctggaggt atcactcttg aaaatggaat ctctgactat
    18601 cacttagaag aagcacttgt tagaaaaaaa atgattgaaa ggtctagaaa atctatagta
    18661 gttgtcgaac ctcctaagtt tggtataaat tcatttgcat cagtagctcc tatagaacat
    18721 atagatacta ttattacctc tgctagtata gaccataata taataataga gttttctcaa
    18781 aaaggaatac ctttagtcat agctgataat tagcttatat gattgatttt tagttccttg
    18841 atgtaataaa ttaaaattaa ggagccaaat aattatttat ttagctccat ttatgtctaa
    18901 actatacttt atattttaat tatgtaacta ctttcagtta gcttatcatt tttagttatt
    18961 tgattgccat tttatttaat ttataagttt tagttattcg attatttttt aattgtctgt
    19021 ctttttaatt tatttttagt tatttaaact cctactttaa aataatttaa atttgactta
    19081 agtattgttt attctacact gcatctttct tcattttaat actttgttct tcttccaagg
    19141 ttaaatcttt tgtcataagt atagcaagtg caattgccaa tactcctcca accaattttc
    19201 caattacaac aggtgtaatc atctctggaa ctactcctgc tgtaaatcct aaatggtccc
    19261 ccaaagctgc tgtagcacaa accagccaag cagtatttac tactttcccc tttggactca
    19321 tatctttcat cattttatat actggaatac tattggctaa agtaaaaact aatcctgctg
    19381 cactagtggc atccatacct aattttccac ccactattgt aagtggttta ttaagaactt
    19441 taactaataa atgtaatatt ggaaaagtac ctaataacac gattgcaata cttccaatta
    19501 cttgaatacc ttctcttata ggtgtcatgc cttttataag tactatacct gttatttctt
    19561 gaaatgctgc tgctcccaaa cctatcgtta tcaatataga tataaatttt ccaaaaacta
    19621 atgacccatt tatcataagc gtagaattaa attttaaacc tactgccaat attactgcca
    19681 atattatcac tggtatcata tttataatta ccattgataa gttaaatcct gctattaatc
    19741 ctcctataat acctccaaaa ggtattgtta tcaagcctat cagtaaccct tttgcaaaga
    19801 atggtctgtc ttcatattct ataagaccta gtcctactgg aattgaaaat actaatgtac
    19861 atccaagcat ggatgacaca accaatcctg caagcattcc tgcctcttta ttgtgtgcaa
    19921 gttccatagc caatggatac cctcccatat cattcgctaa aatagttgca aacatagatg
    19981 ggtcagctcc acagagattg aagattggta ttattatagg acctagtaca tctgaaatta
    20041 ctggagctaa acaaactata ccaaccattc ctaaagccaa tggacccatt gaattaaacc
    20101 cttcttcaaa ctgctcgcca agtccaaatt tatttccaat tattctatct aacgctccta
    20161 aaagcactcc cacagccatt atgtataata taattttatc aaagggcatt tttaattcct
    20221 cctatacatg taaaatactc tattgtaact taattagcac acaccttttt ggtatcgttt
    20281 tcccaattca tagctctttt tactgcttgg ttccatccaa gtaatatttc ttctcttttt
    20341 tgagtagtca tatttggaat atattttttg cttattgtaa attcacttct aatttcatca
    20401 atactgttcc agacaccaca agaaagacct gccatatatc ctactccaag tactgtcgtc
    20461 tcaggatttt ctgcaatttc aattggtata cctaatatgt cggattgaaa ttgcattgta
    20521 aagttatttt tagtaattcc accatctact ctcaacattt ttattttttt ccctgaggct
    20581 aagttcatag aatcaacaac atctttgact tgaagtgcaa cagactcaag agtcgctctt
    20641 actatatgct cttttttagt acttcctgtt attccaacca ttgtacctct agcatattgg
    20701 tcccaataag gtgctgctat acctgcaaat gctggtacaa aatatacacc tccagtactt
    20761 gatatggaat ttgccatgtc atcagtttct gaataatttt ttataactcc aatgccatct
    20821 ctaagccatt gtattgcagc acctgctata tacactccac catcataagc atatgtaact
    20881 ttatctccta ttttccaagc aacagttgtc aaaataccat tacctgctat atttagttcc
    20941 tcacctatat tcatatatag gaaacatcct gtaccataag tatttttaac cattcctggt
    21001 tcaaaacatc cattgccaaa taaagctgct ggttggtcta ccattgaagc agtaattttt
    21061 atagaaaccc cacaaaatgc ctccttatct gtagttccaa aatcaccact cgtaggcatt
    21121 atatctgcta aaatacttct aggtactcct ataatgtcaa gcaactctct atcccaatcc
    21181 atatctctta tattaaaaag cattgttctt gctgctgtag atacatcagt taaaaaagat
    21241 tttcctcctg tcattttcca tattaaccaa ctatctaatg ttcctgcaag tacttctcca
    21301 tttttaattt tttcttttac accttttaca ttatctatta cccatttaat ttttgtacct
    21361 gagaaatatg ggtctataat taacccagtc ttatctatta tcttattttc taaacctggt
    21421 atattcttta aattttccac ataatcataa gttctcctgc actgccacac aatagcattg
    21481 taaagtggtt tacctgtatt tttatcccat aacattactg tttcaccttg attatctata
    21541 ccaattcctt ttatatcact ttcattgatt ccattttcaa aagcctgttg taatacatta
    21601 ttactcacag tttttgtatt attccaaatt tctatagggt catgttcaac atatccagat
    21661 tgtggatata tttgagtatg ttccatataa tcgctaaaaa caaactctga tttttcgttt
    21721 atcaatactg ctcttgttcc tgttgttcct tggtcaattg ctaatacata tttcacttta
    21781 aatgtccccc tttattttca gaattaataa attctttttt attttttgaa tatataatct
    21841 attcaagtta tttatatttt atatcatcat gtggatttat gcaatattca ttacaatatt
    21901 ttttgttatt tttaattata gtgattaaaa aagtattttt attgaaatat tgttgcaata
    21961 tttattattt tattctataa tctagttaaa ataacacata acattttaat atttacacat
    22021 aatcagtcat tcaaaaattt aaatttaact ttttaaggag gctttacaat gaaaaaaaca
    22081 tatctattca gtcctggacc agtaatggtt acagataaag taagaaattc acttcttcac
    22141 tatgatatat gccatagagg aaatgagttt atggatttat tcaaagatac acaacaaaaa
    22201 atcaataaac tatataatgc aacttctaat tactactcat taattgtatc tggctctgga
    22261 acatctgtaa atgaatgtgt actatcttct atctttgaca aagaagatgc agttctgcta
    22321 gttagtaatg gtgtttttgg agaaaggcta gaagaaatat tatctactta taatgtaaaa
    22381 acatataaac ctaattttga atgggcagaa tacccggacc tagacatttt agaaaaatac
    22441 ataattgaaa attctgatat aaaagtaatt gctatggtgt tccatgaaac tagttcagga
    22501 atgataaatc cagtccctga actaggaaaa cttgctaaga agtataacaa aatatttttt
    22561 gtagatgcaa taagtgcctc agcaggtgaa tatattgatg tggaagagtt taatatagat
    22621 ataataacag gtgttggtgg caaagctctt ggagcatttc caggttctgc atacctttgt
    22681 gctaaagaga gtattttaca aaatattaaa gaaaatcaat gtaagaatgt gtatcttaat
    22741 ctttataaac actataaagt tgctaaatca tcttctcaaa caccaaatac acctaatgtt
    22801 actttaatat ttgcccttaa tgaagcttta actgaatttt tggaagatga cagtaaaata
    22861 gaaagataca aagaatgttc tgctatttta aggaatggta tggaagaatt gggtctaaca
    22921 tttttacttc cagataagta tatgtctaat acagtaacat ctgtatttct acctaaagaa
    22981 atcaatgtaa atgagtttat attagggcta gaaagggaaa gtggctatgt tgtctatcca
    23041 ggaaaaggca aattcctaga tgctaatata ttccaagtag caaatatggg agaaatatat
    23101 ccagaagatt gttataaatt cttagatatc ttaaaatcaa aattggagat aaaaaaagtt
    23161 atataacagt ttgtttgtaa aatcctacaa tatatcagta taaattaaat agactgattt
    23221 ttttaaattt tcctataata aattatattt tatatttttg tataggggtg aactataata
    23281 tgtaaagttc acccctattt caaatctttt tccttcaata taatcttttt cttttaattt
    23341 attttatatt tttgacgcaa tcgtttttta tt
//
LOCUS       DAEORZ010000050        23058 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEORZ010000050 DAEORZ010000000
VERSION     DAEORZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.050"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            34..234
                     /locus_tag="KRH39_001820"
     CDS             34..234
                     /locus_tag="KRH39_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG8511729.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     repeat_region   597..760
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=599..626
                     /rpt_unit_seq="tttacataccacatagttgatataaaac"
     gene            982..1164
                     /locus_tag="KRH39_001821"
     CDS             982..1164
                     /locus_tag="KRH39_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBG8511730.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIIF"
     gene            1235..2080
                     /locus_tag="KRH39_001822"
     CDS             1235..2080
                     /locus_tag="KRH39_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG8511731.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(2304..2468)
                     /locus_tag="KRH39_001823"
     CDS             complement(2304..2468)
                     /locus_tag="KRH39_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511732.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(3010..3342)
                     /locus_tag="KRH39_001824"
     CDS             complement(3010..3342)
                     /locus_tag="KRH39_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBG8511733.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(3586..4227)
                     /locus_tag="KRH39_001825"
     CDS             complement(3586..4227)
                     /locus_tag="KRH39_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBG8511734.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYDRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKQNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(4208..4501)
                     /locus_tag="KRH39_001826"
     CDS             complement(4208..4501)
                     /locus_tag="KRH39_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG8511735.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFIEKGEKDNEKSDK"
     gene            4892..4942
                     /locus_tag="KRH39_001827"
     CDS             4892..4942
                     /locus_tag="KRH39_001827"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511736.1"
                     /translation="MTEYIYYYNNKRIKKN"
     gene            complement(5468..6220)
                     /locus_tag="KRH39_001828"
     CDS             complement(5468..6220)
                     /locus_tag="KRH39_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG8511737.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VAMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVIKLVYQTLV"
     gene            complement(6279..7934)
                     /locus_tag="KRH39_001829"
     CDS             complement(6279..7934)
                     /locus_tag="KRH39_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8511738.1"
                     /translation="MEYFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     YQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIYIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(9207..10285)
                     /locus_tag="KRH39_001830"
                     /pseudo
     CDS             complement(9207..10285)
                     /locus_tag="KRH39_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(10369..11025)
                     /locus_tag="KRH39_001831"
     CDS             complement(10369..11025)
                     /locus_tag="KRH39_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8511739.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            11347..11553
                     /locus_tag="KRH39_001832"
     CDS             11347..11553
                     /locus_tag="KRH39_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511740.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(11706..12134)
                     /locus_tag="KRH39_001833"
     CDS             complement(11706..12134)
                     /locus_tag="KRH39_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG8511741.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGDMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(12610..13548)
                     /locus_tag="KRH39_001834"
     CDS             complement(12610..13548)
                     /locus_tag="KRH39_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG8511742.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
     gene            14434..14793
                     /locus_tag="KRH39_001835"
     CDS             14434..14793
                     /locus_tag="KRH39_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8511743.1"
                     /translation="MRKKKDTHSFDFRPLGLAIREAREKAGLSRNDLGDKVFYGERHI
                     ADIENIGKHPSFHLFHDLVTMFNISVDEYFYPAKNVEKSTVSRQIDSSLDLLSDDELK
                     IIQGTIDGILNSRENKK"
     gene            complement(14891..15102)
                     /locus_tag="KRH39_001836"
                     /pseudo
     CDS             complement(14891..15102)
                     /locus_tag="KRH39_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15215..16537)
                     /locus_tag="KRH39_001837"
     CDS             complement(15215..16537)
                     /locus_tag="KRH39_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8511744.1"
                     /translation="MNQKNENYWLLDAPVTKAIWHMAIPMMLGMSINIIYNITDTFFI
                     GRLNDTAALAAISLLLPFTTILMAIGNLFGTGGSTLFSRLLGSENTDRTKQCSATTLW
                     LSFLFGLLTAIISIIFSNYIIRLLGADSNTFAYVKQYLIFYGMGAPFIIANFTLEQLI
                     RGDGKSVESMIGMMISIGANIILDPILMFGLQLGIRGAAIATVIGNAFAVIYYIVCIQ
                     RADNQLSALPKYFRLEKQMLKEIFLVGLSAMLLDILLIVSSLMFNYYALKYGDYVLAG
                     FGISQKLVQIVDLIGMGLYMGVIPLIATAYGARNELRVKEIIKKTALYLALVITCLFT
                     ILFTCRNFIVHCFSNDSDVIRIGAYILTVQLCSSFFAAGAGLLTGIFQAKGEGTPAVV
                     MSVMRGIMLIPAIIFGNYLFKMNGVIFSLLVAEAISCITGLVLYKLKK"
     gene            complement(16626..17279)
                     /locus_tag="KRH39_001838"
     CDS             complement(16626..17279)
                     /locus_tag="KRH39_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8511745.1"
                     /translation="MNNIYSDIYEKLSTLQWIMKRHQMFCQAESGPFADTSRGQGRIL
                     AMLKIQPEIATKELAYLLGIRQQSLNELLNKMEKNGYVERKPSENDKRVMIVHLTEKG
                     KQVQQPKTDYQNIFNCLLPEELLQMSKYLDRIIEAFQLQNGNALEENNMIDWMAQARE
                     RMGDEHFEQLMSMRERAFGHMRPPKDIPGAERFSENYNGYVPDRDGFQPRNFKPDIK"
     gene            complement(17416..17827)
                     /locus_tag="KRH39_001839"
                     /pseudo
     CDS             complement(17416..17827)
                     /locus_tag="KRH39_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(17826..18916)
                     /locus_tag="KRH39_001840"
                     /pseudo
     CDS             complement(17826..18916)
                     /locus_tag="KRH39_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
     gene            complement(19464..20276)
                     /locus_tag="KRH39_001841"
     CDS             complement(19464..20276)
                     /locus_tag="KRH39_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8511746.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFGRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
     gene            complement(20302..20931)
                     /locus_tag="KRH39_001842"
     CDS             complement(20302..20931)
                     /locus_tag="KRH39_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8511747.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVNCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEISCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            complement(21275..22930)
                     /gene="ilvD"
                     /locus_tag="KRH39_001843"
     CDS             complement(21275..22930)
                     /gene="ilvD"
                     /locus_tag="KRH39_001843"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBG8511748.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRMAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNYEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
BASE COUNT         7605 a         4085 c         2842 g         8526 t
ORIGIN      
        1 atatcttgat ataggttaat aggagtattt ttaatgaata atggaatagt gaaatggttt
       61 aataatgaaa aaggattcgg ttttatatca atggaaggta gagatgatgt gtttgctcat
      121 ttctcagcta tacaaacttc aggatttaaa tcattagaag aaggtcaaca agtaagcttt
      181 gatatagtta aaggtgctag aggtcctcaa gcagaaaata taactatatt ataatatata
      241 gatttttcgg gaaagactct ttttagagtc tttttttatt gttaatatat gtaagataat
      301 cattgatgaa gtaaataagg tatattgagt aatgttcttg atagtttata tgctaatgaa
      361 tttcaaaaaa ataagcactc ctataaaaag agtgctctta atgtaaatat aaaccttcat
      421 tggtccagcc taataccccg tattctccaa gttctaactc ttttttatat acattatcac
      481 tttcagccat atctaacaca atcatttctt ctttagaatc aatttgtaaa atatgaggac
      541 tgtgtgttgt gacaattatt tgtgtttttg ggagaattgt ttttaatgta ataaaaattt
      601 tacataccac atagttgata taaaacaaaa acacaaatat ctactttgca agctttgcta
      661 tcctttacat accacatagt tgatataaaa ctcaatactt tgatgcagtt tttaactaaa
      721 ttttcacttt acataccaca tagttgatat aaaaccccaa aataaactta gcatctccaa
      781 tgtttacaca tatacaatgc tcttaaaatt gcagtgaacg cgcaatagtg caattgataa
      841 tacttattat atattctcaa tgccttatat ttcaactgct aaacctcaat ttgtcacaaa
      901 tatcgctcac tgcaaaattt ctatattttt attatatcat aacatataat taatattttc
      961 aattaatttt tacatttaat tatgtttaaa ttctataata atgcaacttt aatgtttctt
     1021 tctggaacta atataaaaac tatttcagaa agattaggtc atgcagatat aaaaataact
     1081 atgaatagct actctcagtt tttagaagag atggacagag aagcatttga aaatttgagc
     1141 aaaatactat ttattatatt ttaaagaaga tgacttattc aatatttgat gctagaatat
     1201 aattaataaa aatttaatta taggtgattg tattatgaat attattaaaa gcataacttt
     1261 actcttaaca ttattaattg tgatattaat gacaggctgt tccccaagag aaagtgctag
     1321 tgaagttgca aaaaaatata ttgataagat gaactctggt gactatgaaa cagcatatag
     1381 tttattaagt aaagatagta aaaaaaaaat tagtatagaa aatcttgaag aatttcaaaa
     1441 tattttagtt agtacaaaaa gaataattag ttataaaata ggtaaagaaa aaatcataaa
     1501 aaaatataaa catgatggaa aagaatataa aaatgttgtt aaattagaag aaacttttaa
     1561 tataaaaaac cttctttata aaaaagatga ttctctaaaa gtaaatagat attttgtagt
     1621 tgaaaataac aaatacaagc tattattgta tgaaagtttc aaaaaagaat ttggtattaa
     1681 tagtatggac cttgctcaac ttaagcttaa tgattttgga ctaaaatact ttaaagaagt
     1741 tgaccttata ctaaaaaaag ctattgctat taatcctact gattcaaagc tttattatgc
     1801 ccaagcatgt aattatatga attttgatgg atttcctggt tattcagata caacatataa
     1861 tgctttagat tcaattaata aatctttaaa gtatttagat aaaaatgatt ctgactacaa
     1921 aaaaaatgca agtaatatat ataacttaaa aggagttatt cttatgtttc acaaaagatt
     1981 tgatgaagca caacaatatt taaataaagc cttatacttt aacaaagata atgaagaccc
     2041 aaaatataat ctaaatacaa taaaaaaaca tctaaactag atatttaatt tttgaattta
     2101 taaaatattt ttttatgtat aattcagcac tgaatcataa tctctactaa actaattaca
     2161 agtttaaatt ataaagactt atttctatat aaatattact ctataacaat aagtttctta
     2221 tttaaatttt aaaatattat ctaaaaatca taaaaaagaa ggtataaaga gctaccctca
     2281 agctcaatat accttataaa tatctaaact tcatttttta ctaatttttc tatatctttg
     2341 ataatatgtt ctttagattt ttttccagct atggcatcaa aaattgcaac agcctttttc
     2401 tctccaaact tttctgctac atcattaaag tctaaaaatc taacactttc ttttggttta
     2461 ccaaacatat aacacctctc atacattttt atatatttat tataacttct atagacttat
     2521 ttttcaccaa taaacgttag acaaattagt ccctttattt ttttaaacta gctttacctt
     2581 caaaattctt aacaaaaaca gtttattcta ctctataaat aaaaatagac aatttaattt
     2641 gtaggaaact gataatagaa tacagtaaat aaaactttgt ttaatctatc tgaaaaagtt
     2701 atctattaaa actgctatga atttataaag aatattctag ttcataaaaa tacaaatatg
     2761 taaatataat caccttaata ttcaatttat ttaaaataaa aaatgtataa aagatttaat
     2821 aatgacaata tactattctc aatacaatct atgatgtaaa taatttaaaa ctactatatt
     2881 atttaaaaat ttatatttat atatgcaatt ctaaaaactt aataagaagt gaacttagaa
     2941 aataaagctt aatttcaaag acttaaatag actattatta ttagtctaaa taaactacgt
     3001 ctatcaaaat tatatattaa attcttctaa aatttttatt gcaaatcttg cgtttggata
     3061 tgtaatagac ccgccaccaa ttacacctat ttttatagct tctataattt catctttact
     3121 tgctccagaa ttaatacaac cctcaatatg ataacaaata cactcatcac atcggcttaa
     3181 aattgaaata gctagtccca ttaattcctt attttttttg cttatagccc cttctgaata
     3241 agtcttatca tcaagacttc caaatactct aaaaaactca tctgtttcgc ttaatttttt
     3301 attgtaaaat cccctatatt ctttcatttc tctagtattc atcttctacc tcctatgttt
     3361 ttcttataag aatatcacac cgtacttata ttttcaatat taaatacaaa ttttcatttt
     3421 atgttcatta gcctaattta tattattttt tgaaatctca ataagttatc atataagtaa
     3481 gattttttca gaactaaaat agaaatctta gtaatttctt tattacctat ctcagttctg
     3541 aaatatatta ttcttatcca aacattatta tagtgattaa ttatattagt tatttttacc
     3601 tgaaatttta tacattaaat aagaatatat aaatggtagt attacaatta caaagattac
     3661 aaagatatta taaaaaggaa tattaataaa tgcattaata attaataata ttccaccaat
     3721 aagccaaatc catccagcta aatggtgtgt cttattccaa ttatcttcat cattcagagt
     3781 ccatggcaaa attattccta ctgagtaatt ttgtttacat ttaggaagat agtttccaaa
     3841 taaaataact attattccta aaattaatcc tgtataaaaa ttagattcca aactaactcc
     3901 tgttgaatat aaaaccatca ttatttgtat aattactgaa agtatagcaa ctccccattt
     3961 tgataaattt tgtataacat ttgaaaaatt agctttctta ggctcattct caagtctaaa
     4021 ccatgtatac aaatggatac atagcattaa caatggtaca ccaaaagctg ctataacctt
     4081 tggagcataa ttatctggat ttccataatt atcaaaatga attgcaactt catttggtaa
     4141 tctatcataa aaatatattg atacaataaa tggtataaaa catattaaag tagataaaat
     4201 aaattgttta tttatcgctt ttttcattat ctttttctcc cttctcaata aattgtgaaa
     4261 accaaagcat aacctcttcg aaaactgaaa tatttatttc gtagaaaata aagttcttat
     4321 actttgtttc aaatatgagt cctgcctttt ttagttgaga tagatgataa gaaattgtag
     4381 cattcgtcat attaaatttt tcactaattt ctcctgctga cattttattc tccctcaaca
     4441 taaccaatat ttctcttctt atagggtctg aaagagcttt gaatgtttct ggaaacccca
     4501 acaccatcac ctcgctattt agaatttcat ctaaatagaa tataacataa catccatttt
     4561 atttcaataa ctatttagaa atttttctaa atagttattt ctaaatattt attgaaaatt
     4621 ttcatattaa gattctcaat ttacttaagt cgcttttatt taatcttact ttatctaatt
     4681 tttttaactt aacaaaagtg tattgcacca taattattaa agcaaaaacc taatgactga
     4741 ggtacttata tataacaata cttttaattt taaattaaaa gtagctaaac ccaatttaaa
     4801 aagtagttaa gtcctattta aatagaaaag aatgaaacga gaaatatact gtataaaaat
     4861 acataaaaac atctactaat tagatacaac tataacagaa tatatttatt attataataa
     4921 caaaagaata aaaaaaaatt aatttagtat aaccttgtta aatatagatt tgaatatgaa
     4981 tttaaagtta tgtaaaatta aaaatacagg aatgaaaatt ccattcctgt ataaaatcta
     5041 acttttgagt ctcagtacaa tcttattttg agacaatctt tttggtttta tattttctta
     5101 tatttaaaat ataacgcata tcttatgcaa catatccata tttttcttta tattctttca
     5161 tcgcatcatt ccaagcgtct tgtactgtta cacctctttt attagcaatt ttttgtgcca
     5221 gttcaattat cttcatagct cttcttgata tattcataaa cattcctcct acaaattacc
     5281 atatatttca tatatataaa tcaaggttat atttttatga tatataatcc ttctattgta
     5341 agattacagt ttatatgtgt attatatatg aactatatta gaagataatt agaattttta
     5401 taaagctata tgattagatt aaatataaaa gctttacaat tacttataaa tttaaaaagt
     5461 ctataagcta aactaatgtt tgatatacca attttatgac cataaataat gccattacta
     5521 aaaatattgg ttttatcatc tttgaacctt tctttatagc aactttagca cctacataag
     5581 ctcccattat catcactaat gcaaacacta tagcaatttt ataatctact tgaccattta
     5641 tcatgaataa taacaatgaa gttatattac ttgttaagtt taatattttt gtatttgcag
     5701 aagcatgtaa aaaatcatat ccatatattt ttataaatcc aaaagtcaaa aaagttcctg
     5761 tacctggacc aaaaaaacca tcataaaaac ccattataag agccataagc atccctaatc
     5821 ttaaattttt cttattaaca gcttcaaaat tatcttcttg acctaaattt ttagaaataa
     5881 aggtgtaaat agccactatt aaaatcatag ccacaaccaa aacatttaat atctcttcac
     5941 ttattgataa tacacatctc acacctagaa cactaccaat aatagtaaat ggtactaatt
     6001 tttttaaaag gttgttatta atttttccag actttgcata tctataggca ctacttatac
     6061 atcctgctga tgctgcaaat ttatttgtac caatggcaag atgtactggc actccaattg
     6121 ccataagaac aggcatgctt ataagtcctc ctcctcctgc tatagaatct acaaaagcag
     6181 ctaaaaaccc ccctatacat aaaaaaataa ctgaaaacat aaaattcctc catcattctc
     6241 tttcaatttt tcttacaata tatattatat aatcttattt aatacttatt atccagcata
     6301 ttctttctat attcatttgg agttatacta ttaaacttct taaatatttg tgaaaaataa
     6361 ctactgttat taaaacctac tttcttacaa atatcactaa tactcatgct tctatctaac
     6421 agatattttt tagattcttc tatcctaaga agatttatat actcagttat tgaaacttta
     6481 tttctttcat taaaaataga actcaaataa tttggactta taaaaaaaac atctgatata
     6541 ctattcaaat ttatattttc tttataatat ttttttatat attcgtttat attttcaatt
     6601 agatttcctt tatcttttct ccaaaatgct ctattcttat caatacactc tttggaaaat
     6661 tcaaataata cattccataa atcttcatca ctgtttacaa gtgatatttt tttattatat
     6721 ttatttctta aataagatat aagttttaaa tcctctaaat ctctaagcac tgttctagac
     6781 aatacaaata tcatttcctg aatagtatct ctaacatcat tatatcttaa tttgtttgta
     6841 tctattagat tataaacacc ttctaaattc atatttttaa tttctttttc aatataaagt
     6901 tctaattcaa agggatattc ttttgaagaa ttattgaaca tatctatcgg attaataata
     6961 tactcttctt ctaataatct actatttgct tttttaagtg cctcttcaag tcttatattt
     7021 tcttttctac tcttcaatat ctcctcatct tttatactaa gttgattttc aaaaaaattc
     7081 ctcttagcta tttcctgaat aaattcagac atcattaata aattttcttt taatacatat
     7141 aatctacttt taggatttaa tggaaattca ttgtaaattt tgtttatgta ttttatatca
     7201 ataccagaac tacttgataa ttcagataaa tttttatcta tataaataga cttatatact
     7261 ataaaattac cacattctat atatccaact atattttctt ctaatactat aggtatctct
     7321 aaactggaaa caccatactc acaatgatac actttttcaa agttatcatt atttatgctt
     7381 ttaacaagtt ttttagtaca cggttttata tttttacaaa tatcgcaaaa tttattttct
     7441 ttcttagtct caaatatttc tttatacttt aagtcatata cagatattct aagcttcaat
     7501 atattagata tttgatttgc aagattttca attatgttta cattaactaa ctcctgtata
     7561 ttgttttcag aaaatactat aggtatttgt ctcaaactcg aaaatttatg aggaacataa
     7621 gtaattaata ttactaaatc aattttacca gtgtttataa tttcatgttc tgaatatggt
     7681 gggcaataaa ctatagatgt ttcctttata ttgacctttt taccatcaat tatttgtatt
     7741 ccttctccct gaatcacata taaaagctgt tcttcactta aatgaaaatg cttttcttgg
     7801 tagtttcccg gaaaaaatct tacaattcca gcactaagtc tattgaattg tgtttttgat
     7861 ggttcgtgta tccacataac ctctccccaa tcaaattttt gtacttcatt atttgactca
     7921 ctaaaatact ccataagctc acctcttagt ttttgtctaa atattttttc ttcccttctt
     7981 atattaaaat aatatcatca tcattaactt tcattactat aaaatcatca tactctttta
     8041 atactcctgt aattttagtt gcacttagtt tactacaatt cagtttttta atatattgat
     8101 aaattctata attacttttt tattttgtaa aatcagtgtt ttcccaaaat aattatatct
     8161 ataaatgaaa gtatttttac tgcttacatc aatatatgtt atcataattt tcctttttat
     8221 tgcatatata aataactgta tataacattg tccaatacta tatagaaata catctaagaa
     8281 agaatattat taaattgttc caattagtat tatttttctt atagaaataa aaaaactgtt
     8341 ggagtctttt agaaaaatta ttttttacat accttttttg cttagatagt ctatcttcac
     8401 agctattttg tatatacact cttctagttc atgattttat tttaatatct tgcttatatt
     8461 gctgaaataa ttgaattctt ttatctatca tattgtctct tatatcacaa acatattgtt
     8521 tcttatatca caaaaatgaa agcattctta tatcttcaat atataaacct gcttttttta
     8581 atcttgtaat aaatatagga tactctatat tctcttccta gaaataacta ataccactta
     8641 tttttctatc atatagaaag taataaatct ttttcttaat aaaattcaat agtaagtgga
     8701 ataaaatttt atatgaataa gaacattata aaaatacttt atacaatagt caataaaaga
     8761 tattagcttc aaattgtgtt gaagttgtat tatttaaagt ggaaaagcaa ataaaaaaga
     8821 aataatggta taagtagaaa aatatactta aaacaaggcg aagcctctct tgtatctaaa
     8881 tcaagtagag aattaaatct aaaaaagaaa ataaatataa aagatgactc ctgtacatta
     8941 caagaatcat cttatggttt cataatcact ccatggtttt taactgtctg atataaaggt
     9001 ctcagttcac tcatagcatg accacccttt ccgattgctg accccgtcac ttatggacaa
     9061 aatgtccata agtgatagtc ctctcttaaa accctctaaa tagatatatc tcaagcacct
     9121 cattgaaacc agttgctgga aacaaactga agcgttctgg aatagtaaaa gaaattgcca
     9181 ccatatccac gttactgtcc tagttgttac tttgctaact ttggagcgat ttcctctaac
     9241 aatatgcagt caaattctgc ctttttgcta agaaatggca gatggtctgc atctggtata
     9301 aaaaaatctg tttgtctggc gccactattt catcaaaaaa tttcttcgct ggaagatagg
     9361 ggctattata gtcattttct cccatgatgt agtatacagg aacctgatag tcaactccat
     9421 aatttcttat gtcagcttct tccactaaaa accgcaatac atcaccttga ttgtcaatcg
     9481 catttggatt gacaatggtt gtatttttta actcgctcaa tgaataatac ggggttgcca
     9541 gtatactgat tatattgccc aaatccatat ttatagaaag cccatacttg ctttggtatg
     9601 ttcttactgt cataagagcc gccaaatagc ttggaccata ttttgctggc ggatatggtg
     9661 caacagcttc cagcgccttg atatcttttt gctttcctgc agctttcacc atttcaagtg
     9721 tcttttcata gcccacgcgc tcattgtcca ccatattaac ggtctgcgca atcccaatgt
     9781 acgcgctgta atcctccgga taggtttgca cagccatagt tcccagcacc gttccccagc
     9841 tatgacctat aaggataatt ttctccttgt tgaattgttg cttcaaatat gctgtcacct
     9901 catggacgtc ttgcagaaca cgctccgcgc tcatggtctg caaaactgct gccggatcgt
     9961 tcgcaaagta tgttttgcct acgtttcgct gatcccagtt caccacagta aaatcatttt
    10021 cccactcata ctggtacaga tgtaaaaatg gcatcattga attaccagga cccccatgaa
    10081 cccaaaggat gatggggttg tctgcgtttt gtccccggat atataacgcc tgctgtatgc
    10141 cgccgatctc caccatttcc atgcggtcga ttccaggagc ttctatttta tggcttgtac
    10201 gtataatcgt gggggctatc agacgcaaac cggcaaacgc cagagcaaaa acagcaacta
    10261 gtatgcaaag tgtgattaat atcattttaa gtaccttctt tcatgaattt ttttagccat
    10321 agatatctcc tctaacatta aaaaaaatat gttgctattt tagttttgtt actctacctg
    10381 tcccattggt ttcctttcat aaaaaatatt aattgattct ataatctcat cttccaggat
    10441 atcaactcct atttgtctca taagaaactg taagaattga aaccgtttac gaagtgtagg
    10501 aaagtccccc tgaaatacat ctgtatcaca ccaaaagtta aagagcagca aaattacctc
    10561 tgcgcattca tctgggaact ctgtgatgat gcttctatcc tctatgcctt ctcgtaaaac
    10621 atttgctaat attggtgcta gttttttcaa attatcctgt gtaaatgcca gtatgatatg
    10681 cggactttct atcgcgcttg taatcatgtt actagattct tttatcacct catccgttaa
    10741 atgccgccta actaaacttc taagcttctc ttttgctgta agtccatgca tttcaccgag
    10801 ccattgattt actgtctcta ttatctgttc aaagcgcctt tccatgacca cattaaaaat
    10861 atcttctttt gaactgaagt gatgaaaaat tccgcccttt gacattcccg aagcatcaac
    10921 aatattttgc atacttgttt tgtcatatcc tttttcagca aacaactttg cagaaatggt
    10981 aatgatattt tcaatagctt gctttggatt gttctttcta gtcattatat aactccttct
    11041 tattaaaaac cgactgttgg tttttattat ataagctatt cctgcttctg tcaagtaaca
    11101 ttgtgtttat ggcaaaacaa taaggtttgg tggttagaca ggtattaatt agactaatgc
    11161 gactttcccc tagatggaac ccttggtaaa tttgcaatct ttaccggctt tgattatatc
    11221 tgtaagcaag gcaggtatgg ccacacctgc cctgtttgcc cctcggatat tcattcctat
    11281 ccctcctctc cttagccatt acaccaaaac gccgctcatt gtctccaatg gcggcgtttt
    11341 aaagatatga tttctcatgc tgctgaaata tgcaaaaaat ataagatgcc ctttattatt
    11401 tttcctacaa tagaaaagaa aagtattgaa gaatgggtta atattggagc aaatatgctt
    11461 tgtttggcac aagatactac ctttatcact tatgttatca atcaattatt atctttttat
    11521 aatgagattg taccaattca aaaacaacat taaattataa atctaatttt atattaaaag
    11581 agactaatat tttatctatc tctattaagg atattttctt atgtatcata atattagtct
    11641 ctcttattat tatatactgc tatcatcatt agttgctatt tatactattt ttataatata
    11701 caaatttatt cagctaacgt tttgcctaat tctatacact catcttttgc ttcttcattt
    11761 gggtcttcca ttgctgtaat tccttctcca ccaataattg tagcaccagc attttgcata
    11821 cgttcttccc aatctctcat ccattcacaa tttccccatc catatgaacc aaataatcca
    11881 atctgtttac ctgataccat agattctaat tctgaaacaa atggctccat atccccctct
    11941 tctagttgct ctgctcccat agatgggcaa cctaatgcaa atttatcttc ttcttttaaa
    12001 tcacttggtt tcagtaagct cacatctaaa acttctggtg tcttaccttt taacttcaca
    12061 ccttcagcca caaaatttgc cattttttca gtatttcctg ttccactcca ataaacaata
    12121 tatattttac tcatttttac ctcctgtagt attttttaat taatcaatag ttagttatag
    12181 ctaactaaca tctaaaaaac aaaagatttc tttatctgaa atctctttat ttttctaaat
    12241 atagaatatc acaattgatt ttgaaattca atacttattg ataatattta tcatttatac
    12301 tttatattca ttttataata tcaaaacact gtatcgtatt tctaatcttt catagaaaaa
    12361 tttctaactt atagtacttt tgcatctctt ataaatcttt tatatgtata agtacataga
    12421 aaaacacacc ttttccagat gtgctatcta taatatcgta tatttatttt tcaattctaa
    12481 ctagctaata tgtttataaa ttttatcact taataccttt atacattgat gtaaaatctt
    12541 acacctaagt aatttttatt atttgtctcg actctatata atatagctat tttatcatca
    12601 aatcaaaaat tatcttttgc tattattctt gtatttataa agatttaaat ataagtatcc
    12661 atttccttgt tccgttctag gtattccaag cgctctcgta cattttatta attgtgcata
    12721 cagttcagct tcatctaaat ctctttcaaa ctcctctctt gcccattctt taattagtgc
    12781 taatgagcct gatacgtatg gtgcactcat actagtacca ctcataatag caagtttatt
    12841 atccatataa gtcgatataa tatttcttcc tggagccacc aaatctattg tagtatttga
    12901 attactaaac ttttcaacca aatagttttc atttattgca cctacttcta ttacctcagc
    12961 ataactggct ggataactat actcacttgt actagaatca ccatctccat tattacctgc
    13021 tgcacacact acagaaatat tattttttac tgcttgcatg acagcatttt ttaaattctt
    13081 atcatctttg tttcccccaa gagacataga tataatatca accttgttat ttacagcaaa
    13141 gttaatagca ttaattatac tttgatatgt tccggtacca tctttattta atgcttttgc
    13201 tattaataat ttacaatctg gagctactcc cataacttca ttattataat tagatgcagc
    13261 tataataccc gccacatgag ttccatgacc attaaaatcc tcatatatat ttttatttcc
    13321 attactgtca tcactaaaat ttgcaccacc aattattttt ccttttaaaa gaggatgaga
    13381 tatatcacaa cctgtatcta ttataccaac tacaatattt ttaccagtat aaccttcatc
    13441 ccacattccc ctagcattca tttgttttat tccatatggg aatttatttt ttgcacctct
    13501 tagattttca tttatttcat atggaattag tttcattctt ttattcatca tatatcctcc
    13561 taaaaatttt attgcttata aaaatcatga attttatttt ataataggtt tataaatcaa
    13621 attaaaacct atatctactt atttatattt atgatatatt ttttattttt aggagtccta
    13681 ttttattgta tataaaataa tatttagata actatattaa aaaatacatg tttgcttata
    13741 tacaaaactt actaaaatgt ctttaatatt tttgtataca aacattatat ttttattata
    13801 aaaatacact aaatttctga ttatcttttc ataagttggt aaatgccgat atatcctgta
    13861 tttttcaagt tcttcatcaa gcacacggat ttccgtactg cacacatcga atgtcgtata
    13921 gtcactttcg taatcatccc agacaatcaa tttctcaata acaatttctg gaagctcaca
    13981 aaagggtact tcctttgcct gtcatctggc taattccttg cggtagttac ggacactgcc
    14041 tttcacaacc tttttgagct tacagtccaa ctgacactgt atcgtctttt ggaagtctga
    14101 tggtttcaaa atcatacccc ctttctctat gaagtgaaag gtatgtatcc ctctttctgc
    14161 accatatctg tacagcaggg tgtgatttgt tgcagaattg aaaaactttt tgaaaatatg
    14221 aaaacagcct atctgcacta ataacaaata gactgtattt aaaacatctg atatatgagt
    14281 ttttaattca tgctcaaggg ctgatgataa actaaaaaaa aggaataaat tcggttgtcg
    14341 gtgccggctg cctgttttac ttagaatcac aaattgctcc ttgagcagag caaacctgtg
    14401 aaaatagtat aaactcatgc taggtgatta aaaatgagaa aaaagaaaga tacgcacagc
    14461 tttgattttc gtcctttagg acttgcaatc agagaagcca gagagaaagc agggctttcc
    14521 agaaatgatt taggcgataa agtcttttac ggagaacgtc atatcgcaga tattgaaaat
    14581 atcgggaaac accctagctt tcatttattc catgatttag ttaccatgtt caatatatct
    14641 gttgacgagt atttctatcc agcgaagaat gtggaaaaaa gcacagttag ccgtcagata
    14701 gattcgtcac ttgatttatt gagtgatgac gaattaaaaa tcattcaagg tacgattgat
    14761 ggtatcttga acagcagaga gaataagaag tgattcgctt cttattttct ctgtttttct
    14821 gtgcagattc tttaacgctt atttgttgat atactccgca ggcaacaaat agtcgtaaat
    14881 caaacttttg tttttttgta atcttgtaaa tatattcttc tggtgtagga cgtttattgc
    14941 caaaatactt tttgaaattt gcctgtacct ctggggctgg actgttcatg tcttcatcaa
    15001 tagtggcttc tatattagcc cgaatatcag ctaatgatac atctccagct ctatcaccag
    15061 ttttcaacat ttttttatat ttcttttttt aagcccaatc atatttttcc agttcctacc
    15121 aatctatact cattctatag aaatctctta atttattgtt gatgattttt ataaccatca
    15181 ctaactttgt taacaagtct gatggagttc agcattactt tttcaattta tataatacaa
    15241 gccctgtaat gcatgaaata gcttcggcta caagcagaga aaaaataact ccattcattt
    15301 taaagagata attgccgaag ataattgctg gtatgagcat aatacctctc ataacggaca
    15361 taacaacagc cggagttcct tctccttttg cctgaaaaat tcccgttaga agacccgctc
    15421 ctgccgcaaa gaaagaagaa cagagttgaa cggttaagat gtacgcacct atacgaatta
    15481 catctgaatc atttgaaaaa caatgaacaa taaagtttct gcatgtaaat agaatagtaa
    15541 acaaacatgt aattactagt gctaaataga gagcagtctt tttaatgatt tctttcacac
    15601 gaagttcatt tcttgcacca taggcagtag caataagtgg aattactccc atgtaaagtc
    15661 ccatgccgat tagatcgaca atctgcacaa gtttttgaga aatcccaaat ccggcaagca
    15721 cataatctcc atattttagt gcatagtaat taaacataag gcttgaaaca atcaaaagaa
    15781 tatctaacaa cattgcagat aatccaacta aaaaaatttc ttttagcatt tgtttttcaa
    15841 gcctgaaata ttttggaaga gcagataact gattgtctgc tcgttgtata cagacaatat
    15901 aatagataac agcgaaagca tttccgatta ctgtagcaat ggctgcccca cgaataccaa
    15961 gctgtaatcc aaacatcaga attgggtcaa gaatgatatt agcaccaatg cttatcatca
    16021 ttccaatcat agattctaca gatttaccgt cacctctaat taactgttct agcgtaaaat
    16081 tggcaataat aaacggagca cccattccat aaaaaataag atactgtttg acataggcaa
    16141 aagtattact atcagcaccg agaagtcgga taatatagtt actgaaaatg atggatatta
    16201 ttgcagtcaa tagtccaaac aggaaagata gccataatgt tgtagctgaa cattgttttg
    16261 tcctgtccgt attttcactt cctaacagtc gtgaaaacaa agtgcttcca cctgttccaa
    16321 acaaattacc aattgccatt aaaatggtcg tgaaaggtaa cagcagtgag attgcggcaa
    16381 gagccgctgt gtcatttaat ctgcctatga agaatgtatc tgtaatattg taaataatgt
    16441 ttattgacat tccaagcatc attggaatcg ccatatgcca tatagctttt gtgacaggtg
    16501 cgtctaaaag ccagtaattt tcatttttct ggttcatttt gttcgtctcc ttttcaaaaa
    16561 ataagcccta tggagttcaa aaaatctcca tagggcttat tgctgtaatt ttacagtgcc
    16621 ttttgttatt tgatatctgg tttaaagttt cttggctgaa atccgtctct atctggaaca
    16681 tatccgttat agttttcaga aaagcgttca gctcccggta tatctttggg tggtctcata
    16741 tgtccaaaag ctctttcacg catagacatt aactgttcaa aatgctcatc gcccatacgt
    16801 tctcttgcct gagccatcca gtcaatcata ttgttttctt ctaaagcatt tccattttgc
    16861 aattgaaaag cttcaataat acgatctaaa tatttcgaca tttgtagcaa ttcttctgga
    16921 agtagacaat taaagatatt ttgatagtct gttttcggtt gctgaacttg ctttcccttt
    16981 tctgtcaaat gaacaatcat aacgcgttta tcattttcag atggttttcg ttctacatag
    17041 ccatttttct ccattttatt aagcaactca tttagggatt gttggcgtat tcccaataaa
    17101 tatgccaact cttttgttgc aatttctggc tgaattttta acatagcaag aattcttcct
    17161 tgtcctcgtg atgtatcagc aaatggacca gactctgcct gacaaaacat ttgatggcgt
    17221 ttcataatcc attgcaaggt tgatagtttc tcatagatat cagaataaat attattcata
    17281 gtattacctc cttttcaaca ggtaccttta acaaataata tacaggtacc ttcctaaaaa
    17341 gtcaataggg tacatgtata tttttaaaac ttagtatatg tatttgaaat tgccatagct
    17401 ggtctgctgt aatatttatc ctacaatttt ccaattatcg tccttgtgaa gcacaagctc
    17461 atactgtgag atttgtgtca ttttcgactt gttatccaga tatttcacgg acacactgac
    17521 cttgagatta tcgccatctt tggtaaagac aggatttacc agttccgaaa atacatagtc
    17581 gccagaaaca ggagcaagca caccgtcttt gacataatag gcaagttctt tttctgtagc
    17641 tgtcagatag agtttaaaga atgtttccaa gaaagtagta gcgtctttga gcgtatcgga
    17701 gctgacgctg acatctgctc cctgtgcttt cggttcatac tttgatttct gtaccaccgg
    17761 agcaagggta ggattctggg taatgaccca ttgcaccatc tgtatacaca tgaacggtca
    17821 cggtgtaatt ttctgtgact gccagtttcc aagccatcaa gtgtgacttt gtccacgatc
    17881 ttgatgtctt ttccggcaac aatcattttt tcgcctgttt tcttgtcggt agctgtggta
    17941 tggattgtga tttctttttc atattcatca gtaagtgtcc ctaaatctac gttcactttt
    18001 tttctggata cgaccacctc aaaagctgga atcagcttaa atccggcgtt ggaatcacac
    18061 tttaattcct caatcacata ggtaatgcac ctttactgtc gtctggttca gaagtaccaa
    18121 accatacacc atcctcactg gattttccag catttgtatt taccttacgg aaagcccaac
    18181 ttgaagcagt agagaactga ccgtttttat ctgtgactac aatatgggat tcgccaaatg
    18241 ttgccccctc aaggaataat tgacgttgcc atctgtagag ggtggaattg gaagaagtct
    18301 ttttgacctt ttcacttccc acattcaact tcgcccagaa ctgtccaatg tcctgtcctt
    18361 cgccagtgta gatgtaaccg ccacattcat agcatccctt atttgctttc acaaatactt
    18421 tcgcaccagc gtaaacttta ttctgtaccg cctgtgagat ttcattatag gaagctctaa
    18481 cgttatcaca ttgccagccg agctgttcac tcaatctctg ccagacaaca cactgttcca
    18541 ataagtaacc ctgcttgtaa ttcaagtttg tatgagaagc agtatattgc ttgatgtact
    18601 ctaaggaaag agcaatgtcc gcaatctgat cgctactcat atgggagctt gcgtcatacc
    18661 ttgttttata tccatttttg aaatttttat tgatgtccac gcaataggca gtttcgccct
    18721 caactttcat gtgtccctca tttaatgtgg atttgataga accatcattc ataacatgtt
    18781 caatgtagcc gacacgttct gctgattctg tccagtattg attgccagaa gcatgaacca
    18841 ccgtcacagg caatgcggta agaatggttg caatggcgag cgtccctgtc agcaatcgct
    18901 ttactagctt attcatattt tctctttctc ctttcgattt tgggtatgaa aaaaagacgc
    18961 tctgaaaaga acgtctggaa atagaaaaga aacgccaaaa gtgtacgttc catagtctat
    19021 ataagtattc aatttttact gatgtgccat gccatatctt tgcatatctg ggaaataaga
    19081 cgtatcaagg ctcattcatt agtagtaaat tcgtagtaat atcacgattt atttcctatt
    19141 gaatatgctt gtagatacag tgtttatggg gataataaga tttttagggc gttttttata
    19201 taaaaatctc ataaaaattt tgattatatt ttcataagtt aacaaatgtg ggtataatat
    19261 gaatttacaa aattcctctg tttgtataag tgctttacca atacactaat atataatcac
    19321 attgaagcta tatttgtatt tatagtagat aatgtatcaa aaattggtta tgaatagtaa
    19381 aaataccagt taagcatatt ttcatgtttc tatttttaac attaaatttc caatcttcat
    19441 atgtatattt ttaacaaagg tgtttactta tttaaaaact ctaaaactct ttcattaaaa
    19501 tcctgtgctt cttcatatgt agcgtgtccc aatccctcat agataaatat ttcgcttttt
    19561 ttaatttttt ctgctaaatg aaaagaagca ttatttccaa caattttatc attagcacct
    19621 cctataatta aggttggaca tgttatttta tttaattcgg aaaaggcatt atgttctatg
    19681 caagaggtcg cttgaataat aaatcgttta aaatcctttg gtttacctac ttttcctaaa
    19741 aaaggaataa ataattggta tttttttaaa tatctttcag aataggattt ctttgctgtg
    19801 tcaatcatta aatcattgta attctgcttc tttgccatat caatccaact acaaataaca
    19861 ttttggatag tatcattttg ctttgaagaa gtaactgcca acacaagttt ctcaactaac
    19921 tcaggataat caattgccag atattgagct atcattccac cttgagaaac acccattact
    19981 tctgctttca taattccaag ttttttcatt atatctgctt ggtctttagc catatccctt
    20041 gtggaatatt tttctgtaat ttgatttttt ctaccaaaca cataaacctt ataatctttt
    20101 gcaaattgct tatatctaaa agcaaaagca atcgcctgta tttttccatg taaaggaaat
    20161 aaaccatctc ctaatcctgg taaaataatt agtgtcttct taccactacc aaattcaata
    20221 taattcactg tattacttcc tatcatcatt tgacaacttt ttgcattata aaacatagtc
    20281 tattcctcac ttttcctatt atcattgttt aaataaatct ttaaattttt gaataccgta
    20341 ctcatcaatt agaggaatat ccattttttt taacatgaat aataaaaaat ccaacctatt
    20401 acatacttca ctgtattcac gtttaaataa tacagggtca agtaaaatat caatactaaa
    20461 aacgagcatt tcagctaatt cctctggata ttcacaagaa atttctttat tttcaacacc
    20521 ttgtttaatt atcttagcaa tattgggtgc aactacattg ttgagattac gaacttcctc
    20581 tattaaagcg aatgggattt tttctaacca gctacaattt accatttttc tacgtgtgta
    20641 atcattcgca ctatgaacaa ccaattctat tattttttca tttgctgtaa gggtgtcatc
    20701 agcaacaaga tgctctaaaa aattattcat ttgtgagtaa agttcttttg tgacacttct
    20761 cagtatatct tctttagttt taaagtgatg atacattgca cctctagata gaccagataa
    20821 attgataata tcttgtgtag atgttgaatc atatccttta gacgaaaata gtgaaaatgc
    20881 tgattggata attttttctt tactattttt acttttttct tctctattca tatttccctc
    20941 gtaaaaacag actgtctgtt tgttttttat tatacatttt aatatttatt atgtcaagaa
    21001 ttaactgact ttcaatttct tcatagttta gatggaacga aaagcattcc acatcaaact
    21061 tttaaaaagt tgtattccct caacaaggtc taaaagatac atctctatgc tgtactcgta
    21121 ttccttataa tagagatgta ttgttttcaa agataatcaa gattttgctg tgtttttcat
    21181 acaaaataaa aaggatacct cattagtgct ataacaccta agatatccaa ttaatatttt
    21241 cttaactttt tctaatttac ataaatatag tctactattt taaaactgct cctgtatttg
    21301 ctgatgttac cagtttagca tatctactta agtatccatg ctttattttc ggttctaaag
    21361 gtttaaattt taattttcta ttttctattt caacttcatc tacttttaac tctaattttt
    21421 tgtctggtat gtttatagaa attatatctc cttcttcaac taacccgatt aaaccacctt
    21481 ccatagcttc tggagaaata tggcctatag atgctcctct agttgctcct gaaaaacgac
    21541 catcagtaag aagtgctaca tgcttatcaa gtcccattcc tgcaactgct gatgtaggag
    21601 atagcatttc tttcattcct ggaccaccct ttggaccttc atatcttata actataacat
    21661 cacctttgtt tattttttta ccaaaaatag catttacagc ttcttcttct gaattaaaaa
    21721 ctcttgctgg tccttcatga actaacattt cttctgcaac tgctgacttt tttacaacag
    21781 ctccatttag agcaagattt cctttcaata ttgcaagccc accttggtta ctatatggat
    21841 tctttaatgt atgtattact tcttcatttt ctatttcata atttcttata ttttccccta
    21901 tagtcttgcc tgttacggtt ttgcaatcta aatttattcc atttatcttt gaaagttcgt
    21961 tcattatagc tggtattcct cctgccatat gtaaatcttc aatatgatgt tttccacttg
    22021 gacttaattt tgttaaacaa ggagtttttt cacttatttc atcaaaaaaa tctaagttaa
    22081 gctctattcc tgattcataa gctattgcag gtaagtgaag tactgtattt gtagaaccag
    22141 ccattgccat atcaacagct atagcatttt taaaagcatc tattgttaaa atatctctag
    22201 gttttatgtc gttcttaagt aactccataa catacatacc tgcatatttt gccatccttt
    22261 ttctttctcc agaatgtgac gcagcagtac cattatatgg tatacccatg cctaaagctt
    22321 ctgttaaaca attcatgcta tttgctgtga aaagaccaga acatgaacca catccaggac
    22381 aagagttcat ggctaacgct tctaattctt cttcacttac tgttccatct ttacatgctc
    22441 caacaccctc cattgcagag ttaaaatcat atacttttcc atttttttta cctggaagca
    22501 taggccctcc actcacaaca atacttggta tattaagtct tgcagcagcc attaacatac
    22561 ctggcactat cttgtcacaa ttaggtataa gtaccaaagc atcaaaacca tgtgccatag
    22621 ccatagcttc tattgaatct gctattaatt ctcttgaagc cagggaataa ttcattccaa
    22681 catgccccat agctatacca tcacataccc ctatagcagg aaattctaat ggcaaaccac
    22741 cactcattct tactccattt tttgcagctt ctgctatttc atcaagatgc agatgaccag
    22801 gaatcacctc attttgtgca tttactatac ctattaatgg tctttcaatt tcttctttag
    22861 taagacccat tccatacatt aacgctctct ttggagctcc ttctattcct ttttttatat
    22921 cacttctcat aatattacct ctttcttact tttttaaatt ttcataatat tattataata
    22981 tatttttcta ttaattaatt acgaaaattg taaaaaaata ttagcatata ctaatgaaaa
    23041 tttattacga ttttttat
//
LOCUS       DAEORZ010000051        22756 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEORZ010000051 DAEORZ010000000
VERSION     DAEORZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.051"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            289..942
                     /locus_tag="KRH39_001844"
     CDS             289..942
                     /locus_tag="KRH39_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG8511749.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            1083..2354
                     /gene="brnQ"
                     /locus_tag="KRH39_001845"
     CDS             1083..2354
                     /gene="brnQ"
                     /locus_tag="KRH39_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG8511750.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2556..3743
                     /locus_tag="KRH39_001846"
     CDS             2556..3743
                     /locus_tag="KRH39_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBG8511751.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSLAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(3973..5997)
                     /locus_tag="KRH39_001847"
     CDS             complement(3973..5997)
                     /locus_tag="KRH39_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8511752.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            6387..7055
                     /locus_tag="KRH39_001848"
     CDS             6387..7055
                     /locus_tag="KRH39_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511753.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            7057..7542
                     /locus_tag="KRH39_001849"
     CDS             7057..7542
                     /locus_tag="KRH39_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG8511754.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            7603..8724
                     /locus_tag="KRH39_001850"
     CDS             7603..8724
                     /locus_tag="KRH39_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBG8511755.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTVVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            9005..10195
                     /locus_tag="KRH39_001851"
     CDS             9005..10195
                     /locus_tag="KRH39_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG8511756.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEISRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    10329..10584
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10643..11644
                     /locus_tag="KRH39_001852"
     CDS             10643..11644
                     /locus_tag="KRH39_001852"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBG8511757.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            11748..12137
                     /locus_tag="KRH39_001853"
     CDS             11748..12137
                     /locus_tag="KRH39_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBG8511758.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            12298..13506
                     /locus_tag="KRH39_001854"
     CDS             12298..13506
                     /locus_tag="KRH39_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG8511759.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(13592..14668)
                     /locus_tag="KRH39_001855"
     CDS             complement(13592..14668)
                     /locus_tag="KRH39_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG8511760.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            14897..15424
                     /gene="hpt"
                     /locus_tag="KRH39_001856"
     CDS             14897..15424
                     /gene="hpt"
                     /locus_tag="KRH39_001856"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG8511761.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            15513..16445
                     /locus_tag="KRH39_001857"
     CDS             15513..16445
                     /locus_tag="KRH39_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBG8511762.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVCIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            16507..17733
                     /locus_tag="KRH39_001858"
     CDS             16507..17733
                     /locus_tag="KRH39_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG8511763.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKIIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            17817..18038
                     /locus_tag="KRH39_001859"
     CDS             17817..18038
                     /locus_tag="KRH39_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG8511764.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            18227..18397
                     /locus_tag="KRH39_001860"
     CDS             18227..18397
                     /locus_tag="KRH39_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511765.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(18490..18951)
                     /locus_tag="KRH39_001861"
     CDS             complement(18490..18951)
                     /locus_tag="KRH39_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511766.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKV"
     gene            19134..19601
                     /locus_tag="KRH39_001862"
     CDS             19134..19601
                     /locus_tag="KRH39_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511767.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            19644..21407
                     /locus_tag="KRH39_001863"
     CDS             19644..21407
                     /locus_tag="KRH39_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBG8511768.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            21415..22023
                     /locus_tag="KRH39_001864"
     CDS             21415..22023
                     /locus_tag="KRH39_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8511769.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            22095..22664
                     /locus_tag="KRH39_001865"
     CDS             22095..22664
                     /locus_tag="KRH39_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG8511770.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         8658 a         2399 c         3884 g         7815 t
ORIGIN      
        1 ataataaaat tattcgtttt tttgtcgatt ttataaaaaa ttaaggttat ttattgaaaa
       61 aaattacttt ttccaataat tgaatatgta tgagtataat gtcaattatt ctaacttgtt
      121 ttatactatt aatggaagtt tattataaat gagtttgtga tataattata aaagtactga
      181 atacatggat atttcgtaaa aactttttaa tatattaaat atgtatatta agttaatata
      241 ttaaaaaaaa taatatcaaa aataatataa tttatagtgg aggggaaaat gactaaaatt
      301 attaaagtgc aaaaacctaa atttacagga gaattagaaa ttattgaaaa tcttgaagat
      361 atacttgaag atatatttaa tgatgaaaaa atatttaata aaattataga aaatgaagtt
      421 ataagtgatt tagattctgt aagagttagc tttgaatctt gtatatttag aaatgtgtct
      481 tttgaaaatt gtaatttaaa aaagattgat ttacttgatg tggtatttga aaactgcaat
      541 ttatcaaata cattttttga tggaggaagt atatacagag ttgaatttaa aaatagtaaa
      601 ttgataggag ttagatttga tgattgtata cttaaaaacg ttttgttcaa ggatgtttta
      661 ggtaagtatt ctaacttttc attctctaaa tttaagatag tttctatctt agattcaaat
      721 tttgaaaatt ctgtatttca agaagtaaaa ggtgacaatc tagtatttga gtatacaaat
      781 ttaaaaaaag caaactttaa tggaactaaa ctttctggca tagattttac tactaataat
      841 attgagggaa ttgagattgg tatagatgat attagaggag caatttttga tgtgtcacaa
      901 gcaattgatt taacgaaatt gatgggaatt atcataaaat gaaaaaaata ttcataaaat
      961 aaattgggaa aacgttaaca atgtgtggat ttttaccaga ttgacatttt tctgaaaata
     1021 aagtaagatt atcttaatat tcaaaatatt ttcaaagggg gtaatttagt gtgagtaagc
     1081 agatgagaga tatttttata gttggttttg ctttattttc tatgttcttt ggtgcaggaa
     1141 atcttatgtt tcctccattt ttagggatgg aatccggcaa agactggtta attcctctta
     1201 ttggatttgt tgttgcagat gctggtatag caatgcttgt tattatagca acagcaagat
     1261 gtaagggaag catggatgat gtacttagga gagctggtaa agggttagca agagtaatga
     1321 gtgtagctgc tctagcatgt ttagcacttc ttgtaatacc aagaacttgc gcaacaacat
     1381 atgaaatggg tattatgcct atatttggag accatggtcc agtagctaaa gctgtatttt
     1441 caataatctt ctttgggcta actttagcat ttactattag gtcttctaag gttatagaca
     1501 taataggtaa gtatttaact ccagcgttat tattagtatt atttgtttta atagtaaagg
     1561 gtataatttc tccagcagga cctatgagtc ctgagcatat gatagataaa aatctatttg
     1621 gtgaaggtat atcacaaggt tatcaaacaa tggacgctct aggtgcagca tctatggcaa
     1681 ctataatgtt gatgtctata atagctaaag gatatacatc agataaagac caaataggaa
     1741 tgactattaa agctggtttt gtagcctgtg tatttctagc agtagtatat ggtggtctta
     1801 cttatttagg tgcaacagtt tcaactttat atgatactag tatacctcaa gcatctttat
     1861 tagttgaaat aactagtata ttgttatatg gtcaagtagg aaaggtaatt ttaggtgtaa
     1921 ttgtggcact tgcctgtctg actacttcta caggtttaac agcatcaata tcaacttatt
     1981 ttgaaggaat aagtaaggcg agttataaac aactggttat aggtatatgt gtagctagta
     2041 tgcttatatc aaatttaggt gttgattcta taatagccat atcagttcca atcctacaaa
     2101 ctatatatcc tgttttatta gcaatagttg ttatggagtt ggctggtaaa catataaaga
     2161 atgataatgc ttttaaaggt gcagcatatg ttacattagt gattagttta ttatcagcta
     2221 taaatggaat gacaggagct gttccattta tacaaagctt gccacttgca ggtttgggct
     2281 ttaactgggt aataccagct atattgggtg gaatagttgg taactttata aaaagcaaca
     2341 aacaagtagc ataataattt gtaattaatt taattttata atatattaat ttttagtact
     2401 atgggtaggt ttaatctaaa cctaatctac ccatagtact ttttagattg gattataata
     2461 gcatgtatat aattttttat aatataattt ttctttgaag tatgataaaa ttagtaatag
     2521 tgataagaat gtacaaattt aggaggtttt aattaatgct tagaaaaata acagcattta
     2581 tgctatccat atgtttaatt attggactta caggttgtag tttacttgta ggtaagaaag
     2641 ataaagagaa aattatagat gattttaaag ttgcagtggt aactcaacca ttatctgaaa
     2701 ataaagtaca atacaatatg gtggaagaga tggcaaaaga atatgaggaa gagaataaaa
     2761 ttgataaaga caaagatggt caaactaaag ttaaacaaac tataaagcat gttgtattgc
     2821 cagagaattt tacttctaat atagatagtg ctattaataa gatagtaaag ttggctgacg
     2881 ataaagaagt tcaggctatt gtagtttcaa ctgaccaagc agggctttta cctgctttac
     2941 aaaaggtaaa ggaaaagcgt ccagaaataa taactatttc tgcccctatg ggagatgata
     3001 aaaaccaatt aagccaattt gttgatgtga atctaggggt tagtgcagag gaaagaggaa
     3061 aagtattagc tgaacgttca aaagaaatgg gagcaaaagc ttttattcat tatgcctcta
     3121 cagatgattt gaaagatgtt aatatagcca aaagattaga aatgattaaa gagacttgca
     3181 aaaatatagg attgccattt gtacaagtta atactccaaa tataaatact gaagaagaca
     3241 aaaacaaagt taagcagttt ttaaatgaag atattgaaaa acaagttaaa aaatatggaa
     3301 aagatataaa tgtatttggt gtaaatgagt atatggatga agttatattg actaaggctt
     3361 tagaattaaa gtatatagtt gcagagcaaa gcaatccatc accaatacaa acatatccta
     3421 gtgttatggg tctaaaaata tctgaaaaag atgctcaaaa ttatgataaa ataaacgaca
     3481 tgataagtga aaaggctaag gcttttggta tgtcaaatag acttggtgga tatcctatgc
     3541 caatggatgc ttttctacca tcattagcaa tttatcttgc tactgaaatg gtgaaacaag
     3601 atttaacaca agaagatgta tgtgacccag attatttaga ggcgtttaca gaattaagat
     3661 ttggaatcgg ttcagaattt actcctttaa cagaggtact ttataattat caatcagtaa
     3721 ttttaagcca attaatttat taatattaag gaagtgtctc aaaatgaact ttttagttta
     3781 tgaggcactc tttttctatt aaatcaaata tatttttatc tttttaacaa ttaaaaagaa
     3841 gcttatccat atgttgagat agcttcgttt ttaatataaa taagttttat tattttataa
     3901 aaaatttata gaaaaataaa aaattataat tatatataaa ttttgataaa gtaaagatga
     3961 attactatag gtttaaatta tattattttc tattattttt atgcctttct cagttattga
     4021 tgagcctttt cttcccttac tagaaattaa gtatccattg cgtgtcaaat attctagcat
     4081 atttcttact tcgtattctg tgacattata ttccataaat ttcattaact ttgtcctccc
     4141 cattggtttc atgtgcaatg attttaggat agaaatgcat atgtttttgt catattggtt
     4201 taaatcattg aatatggaca tattacttga catatgatta ttatttaatt ttgaagatat
     4261 gttttgaggt aaatcattta ttgtaagata attggaaccc atgtaagcca tatattcaac
     4321 acaatttctc agttctctaa tattccccat ccaagtgtat gaatttaaag cttctaaaag
     4381 actcttatcg agttttatgt ttttataggg aagttctttt aaaaatgatt ctaaaagaag
     4441 aggtatatct tctaatctct ttctaagagg aggtaaatct aaagtgaaca tacttatcct
     4501 ataaaataaa tccatcctaa atttattttc tataattaat ttctctaaat ctttatttgt
     4561 ggcagctaat attctcacat caattggaat aatacttgta ccaccaattt ttctaatttc
     4621 tttttcttgt aagactctta aaagtttaac ctgtaaatca tgtggcatat ctcctatctc
     4681 atctaaaaat atagtgccag tatgagctaa ttcgaataat cctttttttc cacctttttt
     4741 tgctccagta aaagaaccat cttcatatcc aaatagttca ctttcaagta attcacttgg
     4801 taatgaagca caatttattg ctacaaaagg tttatttttt ctatttgaat gattgtgtat
     4861 tgactgagta aaggcttctt tacctgttcc tgtttcacct gttattaata taggattatc
     4921 tgtaagagcc attttttttg cctttttaat acaatctttt ataatagaac tatttccaat
     4981 aatattatta aatttatatt tagctacata acctcttttg acagaatcac ttcttatttt
     5041 ttgttctaat atttctatat tgtttacatc ttttaatgta attaagcaac cctctatatt
     5101 tttataaaga taaaatggtt ttttagaaag tatgaatttt ttatctattt cgtctatgta
     5161 tataatcata ttatctatat ttaagttctt tgtaatttta tctaagaaac tctttggcat
     5221 atattccatt aaggaaggtg attttataag tttttcatct agttccaaca tattaagtaa
     5281 agacttattt acaaaagtaa ccttattaaa ggtattgaaa aatattagac catcacctat
     5341 ttcatcaatg gtcatgtaaa gtatagtttt taatttagat atattatcta atgatgtatt
     5401 taagaaatca tgacttagtg tctccttgga caatttgtat aatttttcta taaatttctc
     5461 attttttagt tttaatacta ctaataatga catatatgta tctaatgaga tttttctcca
     5521 acctaaatca acaatatttt ttatgtattt tggtataaat ctaatctgac ctggtgttat
     5581 agctgtatct aaatcatata tttcatcaca atctggatat gtcggtacaa aatctatatg
     5641 atttatacca attttatata gataagtagc catctcaaat gctgaagttt tattggttgt
     5701 cataagtaac gattttgtac ctggaggcag tttcatcatt ttttcatatc caagttttga
     5761 aagtgctttc gttccatgaa taatttcaat atcttcctta aaatgtttta ttaaaaaatt
     5821 actagttaaa ggtgatagag aaagcactac atctccctct aaattttctt ttattccatc
     5881 aagtatattt tgtgtttcta tgattatttc gtctccaaaa aagtttgtta attcttcttt
     5941 gtagtggtca ctattcaatt tatcatatgt tataaggatt aacttctttt tatccatttt
     6001 atttctccta acaaaatagt atttattttt aaagtattta aagtataagg caagttatat
     6061 tatacaacaa atagtagtat aaatttatat atttgattaa ataataggtt tttttaggat
     6121 tttatcctat aaaaacctat tttgaaatta tttttgcaag ataaggaaaa tagcttttta
     6181 tattgtaatg tatattttaa ataattaata aaaaaatatt atatttttaa acttgaaaag
     6241 taagtaatta taagctattt aatcactgga ataaatttac aataaaaaaa taaatacaaa
     6301 ggctttatca ttaaggtatt ggcataagtt ttgcttatta aacaagtata ctaataaaat
     6361 tcatttaaac aggagggaga atttatatga gtaaattaga atcaacaaca gaaaaagttg
     6421 aggtaaaatg gtatgggtat gtagcattaa tattgggagc attatttttt tcaggaatat
     6481 ttaaagatgc accaggacca ttaaaagtat tggattttaa caatgtatta ggttcttttg
     6541 gaacattggg aacaattaat gatggtgtag gtacattagc tgcaaacttt agaggtgatg
     6601 gaggtactgg ccctcgagat ggatggctat atgcattgac attaatacca tcagttatgt
     6661 ttgcacttgg agtagttaga gttatagacc acttagatgg aatgaaggca gctcaaaagc
     6721 ttcttagtcc actattaaag ccattattag ggttacctgg atttgcagga ctaactttga
     6781 tagctagttt acagtctaca gatgcagctg catctatgac taaagaactt aaagatgatg
     6841 ggtatataga tgaaaaacaa aaagctgtat tttgtgcatt tcaattttca ggtgcgtctg
     6901 caattacgaa tttctttgct tctggagcag cactatttcc atttatagga gatgtaccaa
     6961 tatttatacc acttgcttta atattaatta tgaagtttgt tggggcgaac ttacttagat
     7021 tatatttaaa taaatttgac aagggggaag cttaacatga gtgaggtagc aaaaaaagaa
     7081 gtaaaagtta aaaaaggaaa tgtgatagat gcgtttattg gaggagctag aaatggattc
     7141 caaataagta caaattcaat ggctccaaat gtcatcttcg gttttgctat aatttctgtg
     7201 ttcaacttaa caggtctttt ggatgtaata gggacaatat ttacacctgt aatgagtatc
     7261 tttggacttc caggtgttgc agcaacagct ataatgacaa tatttatatc aatgggagga
     7321 gcatctggag taatcgcagg tttatttaca gcaggtcaaa taagtcaagc acatgttgca
     7381 attctattgc caatgttatt gctttcagga gcgcagatac aatttatggg gagactttta
     7441 ggaacagctg agttaaaaac taaatattat ccacatatgt ttgttatagc aactttaaat
     7501 ggttgtattg caatgcttat aatgaattat ttcgtagtat aggtaattat aataaaaatt
     7561 taatataaat ggaaagttta aattttaagg aggagctaga ttatgggaac taacaaagat
     7621 tatattgagt atgtacataa agtgtatgat tatctacaca ctataccaga aaaaggattt
     7681 gaagaatata agacatcaga gtttgtttta aaagagcttg aaaaaatggg gtttgaaaat
     7741 attaataaag gagttgcagg aactggtatt atagtaacta tagattctaa aaaagaagga
     7801 ccagtatttg ctataagagc agatatggat gcattggaat ttatcataga tggagaaaag
     7861 aaaatgatac atgcttgtgg tcatgatggt catacttcta tgcttttaac agctgcaaag
     7921 ttaataaaag agagaaattt agtaaaaaga ggtaagttaa aaatactttt tcaaccaggt
     7981 gaagaaaaat tatatggagc attaagagta attgattctg gtttgttgga tgatgtagaa
     8041 gaaatggtag ggatgcattt aagacctgga caagattgta ggttgggaaa ggcgatacct
     8101 gcaatgtacc attgtgctag ctgtataatg gaatttacga ttaaaggtat cacttgtcat
     8161 ggttctaaac cacatttagg agtaaatgca gtagatgctg tctgtttagc agtgaatgca
     8221 ataaatgcaa taagagtaaa tccagctata ccaaattcta taaaagtaac aaatatatca
     8281 gttaagggag aaacttataa taatatacca gatgaagcat ttatggcttt agatataagg
     8341 gcagaaagta atgatataat agaagaatta aaagaaaaag caatacatgc tgtaaaaact
     8401 gttgtaagtt ctataaatgc agaagctgta ctaatttcca aagatggagt accagcagca
     8461 gaatttgatg aagaacttgt aaacttggct gaagaaacta taaaagatgt attgggagct
     8521 gatgcatcta taggtatatt taaaaattct ggtggagaag acttccatta ttatacacaa
     8581 aaactaaaat gtaaaactac atatattgga ttaggagctg atgctacacc aggatttcat
     8641 aatccaaatg taacatttga tacgaaagct ttagaatatg gtgtagatat atggtgtaga
     8701 ttggttgaaa agagattggg ataacatcca aactaataat tctacattta aaattataat
     8761 tatagaagtt atagaaaaag attgcttatg ttaattagaa aataattaat atgagcaatc
     8821 ttattttgat ataatgaaac tgattttgga atattgaaaa aatatgatgg aaaagtaatt
     8881 atattttaaa tctattggac aaaaaagtga tattattata atattaagaa ttgtaaaata
     8941 ataccaaagt gcatctagta atagacttaa caattataaa aattttattc ttaggaggga
     9001 aaaaatgagt aagtataatt ttgacagagt aatagataga gtaggaacag attgtgtaaa
     9061 atgggatttt agaactaatt gttcaacaaa agcacaaaaa gatggtttgc ctttttggat
     9121 tgctgatatg gattttgaat gtgcagaacc aataatagaa gcattacgta aaaaagttga
     9181 acataaaata tttggatata gttcaaatga tagtgataaa tactttaatg cagtttgtga
     9241 ttggtataaa aggagattta actgggaaat aagtagaaaa gatattattt ttagtcctgg
     9301 tgttgtacct gctgttgcta ttttagtaag aatattaact aattctaatg aaggtgtaat
     9361 agtccaaaaa ccagtgtatt acccatttga agctaagata aagagcaata atagggacgt
     9421 tgtaaacaat cctctgatat atgagaatgg tacttatagg atggattatg atgatttaga
     9481 agaaaaagct aagtgtagca acaataaagt actaatactt tgtagtcctc acaaccctgt
     9541 tggaagagtt tggagagaag atgaattaaa aaaggttgta gaaatatgta aaaaatatga
     9601 cctatggata atagctgatg agatacattc tgatttaatt agaaaaggat ttaaacatac
     9661 tcctttacag tcattatgcc cagagtacaa ggataaaata gttacatgta tagctccaag
     9721 taaaacattt aatctggcag gaatgcagtt gtctaatata atagtgaata atgatgaact
     9781 aaaaaagaaa tatcaagaag aggttacagc tgttggagtt gcaacttcac caaatccctt
     9841 tgctattgta gctacaatag cagcatataa tgaaagtgaa gactggctta atgaattaaa
     9901 tgattactta gataataata tacaatttat tgatgaatac ttaaaagaaa acctaccaaa
     9961 agtaaaactt gtttatccag aaggaacata tctagcatgg ttagatttta ctgcttatgg
    10021 attaaatgaa gtagaacttg aagatttaat gtttaaaaaa gctaatgtat tatttgatga
    10081 aggttatata tttggaaaag aaggaatagg atttgaaaga ataaatgtag cttgtccaca
    10141 gtctttatta aaagaatgta tggacagatt aaagactata tttgaaaatt tataaagtat
    10201 aggtaaaaat ttataaagtg tttaaaaata aaaaaataat aaacttgaca aattttaact
    10261 aatcacttat catactatta tgaataaata gaaaacataa gagagctaaa ttttattgaa
    10321 ttaaactctt aaagactttg aaaggaagag tagttataga atgtaattaa agcgagttgg
    10381 tgatggtgtg agaccaatat gaagtctata gtgaagaaca tcctggagtt gctaaccgaa
    10441 attttaaaag agtaggctta gtcggaacca aaccgttatc atattggggt gtatcgagta
    10501 atatctgtac attgtgagct gggtttgaat ttatttcaaa ctaatcaggg tggtaccgcg
    10561 gaaaagcaac ctttcgtccc ttttatatag ggatgaaggg tttttttaat ggaaaaatat
    10621 ataaatatag ggagtgaatg atatgagcac aataatacca aaggattata aaagtagttt
    10681 gaacgtaatt gatacacaaa tagctataaa aaaattaaaa gatttttttg aaatgagact
    10741 aagtaatgaa ttaaatctta taagagtttc ttctccgtta tttgtattgc cagaaacagg
    10801 tgcaaatgat aatttaaatg gagaaaaggc tgtaagcttt gatataccat ttatgaataa
    10861 aaatgcagaa attgttcaat cacttgcaaa atggaaaaga ttagccctta aaaaatatgg
    10921 gtttgaagtt ggtagtggtt tatatacaga tatgaatgca ataagaaaaa atgaagaatt
    10981 agacaatata cattctcttt atgtagacca atgggattgg gaattggtta tagataaaag
    11041 tagtagaaat gaaaagactc taaaagatgt tgtaaaaaga ttgtatggag tatttaaaga
    11101 tactgaaatt tttgtatgta gtatgtatga aggtataaaa gaaatattac cagaagaaat
    11161 aacttttata acatcacaag agttagaaga tatgtatcca gaattatcac caaaggaaag
    11221 agaagataaa attgtaaaag agaagaaagc agtatttata actcaaatag gaaaaacatt
    11281 aatatcagga gaaaaacatg atggaagaag tcctgattat gatgattggg aattgaatgg
    11341 ggatatatta ttttacaatc ctgttttaga ttcagcatta gaattatctt caatgggaat
    11401 tagagttgat gaagaatctc ttgatagaca attgaaactt gctggatgtg aagagagaaa
    11461 agagtttgac tatcataaga tgcttttaaa tggcgaactt ccgtatacaa ttggtggagg
    11521 aattggtcag tcaagaatat gtatgtattt cttacaaaaa gctcatatag gagaagtaca
    11581 agttggagta tggccacagg atatgattca aaattgtagt agtgcaggaa ttgaattatt
    11641 ataatttaaa tataaactaa ttgataaaat ttatatctaa tatgttcatc aaattaatta
    11701 tatcggttat aatgtagaat aacctataat tataagaggt gataaatatg gagtttttaa
    11761 aaataatcat taatatagtt ttagatattt taaaaaaaat tttggtcaga tttaaaaatg
    11821 ctaaatttgg tttggttttt gtatttgatt tacttaaact acctgatttt atgacagata
    11881 aaagaatcaa tatagttgat aaaataaagg taatttctgt attaattttt actatatcct
    11941 actttgtatc tggggttgat ataattccag agatgattgc aggggcattt ggttttatag
    12001 atgatgctat agttttaatt tggagtatag gtatagtgaa tgaagaaatt aataagtata
    12061 gagttatcgc aaaaaaagat aaacattcaa atataattga aaatgtagaa ttttctataa
    12121 aagatgaaga agaataacaa tttatatatg ttattctttt ttttattaac aaaaaatggt
    12181 taaattgttg acggagaggt aaaactctat tatcataata agtggatatc caatatttaa
    12241 ctaagtattc aatgtgcaag aatgcttaat ttaaaaaagt tatggagggt aaatattatg
    12301 aagaaaaaaa taggtttagt gcccaaacta atcatcggta tcatagtcgg tattttaatc
    12361 ggaagttatg caccagaaat aatagtacaa atattagtaa cagttagtac tttattctca
    12421 gcattcctaa aatttgttat accatttata attataggtt ttgtaacagc aggtatagct
    12481 gatttagcaa caggtgcagg aaagttattg gggataacta cgggaatagc atatggttct
    12541 accttaatag caggattatt atcatttgca gtatctacat taatcttccc aaattttatt
    12601 gatgcaagtg ttgcaagtca aattggagac ccagaggcag gaatgttagc tccaatattc
    12661 acaattccat tagagcctat ggtagatgta acagctgcaa tcgtatttgc atttgtaatg
    12721 ggacttggaa tatcagcttt aagaaatcat ggaaaaggag agactttatt taatttcttc
    12781 caagaatttc aaggaatcgt aacgaaaacg ttgtcaacag tgattatacc tttattacct
    12841 ttatacatag caggaacttt tgcaaatata actatagcag gggaagtatg gacaatatta
    12901 ggggtattct ggaaagtata cttagtagta atacctttac attttgtata tatggcatgt
    12961 caattcacag cagcaggtgt attttcagga aaaaatccag taagaatgtt aaaaaatcaa
    13021 gtacctggat atttaacagc tataggaact caatcttcag cagcaactat accagttaat
    13081 gttggttgtg cagaaaaaaa tggagtaagt aagcaaataa gagaatttgt tgtacctctt
    13141 tgtgcaacaa tacatttatc aggtagtatg atatcaataa caggatttac aactgcagtt
    13201 ttaatgatga atggtatgcc acatggaata ggtgtattat tcccttatat tgcaatgtta
    13261 ggtatagcaa tggttgcagc accaggagca ccaggtgggg ctataatgag tgcacttcct
    13321 ttcctaccaa tggttggaat accatcagat ggtgggcttg caagtttaat gatagcttta
    13381 tacttaactc aagacagttt tggtacagca tgtaatgtat caggagataa tgcaatagca
    13441 gtagtagtag ataaaataaa tgatggaatg agtaaaaaag gaaataaaga agagcaaagt
    13501 gcttagtttt ttaataatca aaaatgagtt atctcaaata gagatagctc attttttatg
    13561 gtataaaaaa ttataaaata aagtgatttt tttatttaaa aattgcttta agagatgata
    13621 ttaataattt attttcatta ggttttaata tacatactct aaagaaatac tcatctagac
    13681 cttcaaatga acaacaatct cttattatta ttttttgtgg aagcagttgc tctcgaagag
    13741 atttagcagt caattccttt gtcttgattt tacacaatat gaaattacct ttagtattgt
    13801 atatatctaa gctttttata ttcttaagct ctttaagcag gtaatctctt tcagtgttca
    13861 ttaaggaaat agtatttgat ataaaatcta aatcactaaa catgatttca cccattttag
    13921 aagctattat gtttatattc cataaatcta gatttttatt tatttcattt ttgacattag
    13981 tgttagatat aagaccatat ccaagcctta ttccaggcgt tgaaaaaaat tttgatgtac
    14041 ctcttataac gaataaatta gaataatcat caactaattg agtacaagag tatgtatctg
    14101 tatcagtaaa ctctacataa gtctcatcaa tcattaaaaa tgagtctgta ttttttaaaa
    14161 tttctctcac ttctacttta gtaaatgcaa atccagttgg attactagga ttacagatta
    14221 ctaccaaatc ataatcttta gcattgatag tttttataag attttctaaa tttatatgaa
    14281 aattatcttc ttcttttgca aagtattttt ctatagaaca attaatttta gataactctt
    14341 tttcatactc tgaataggct ggagagagta aaagagcctg tttagggtta attgtatgta
    14401 taaatgatga aattagttct gtagctccac tgccaagtac tatattatct atagaacatt
    14461 tgcagtaatt ggatatagaa gtttttaagt ctatgtaatc agggtcagga tacatagata
    14521 ccatatctat attgtttaca atgtattgtt tggctaaact tgatgtacca aaaggattta
    14581 tatttgagct aaaatccata aattcttcct ttgaaaagcc atacttacta gataaactat
    14641 ataaattagc tccatggttt gtgctcataa aagtacctcc aaaaataaaa ttatataatc
    14701 gataaaaata actgattaat atatgtacat atagattata atacaaagga atcgttattc
    14761 caaattttta atgtaaaaag caaaaaagta tggataagct gttaatttag gtttaaaaat
    14821 gatgaaaaag attttaattg atattttggt atgctataat atataagtta atagaaggaa
    14881 agcgggagga tacaaaatgt acaaagttac aggaaaaatg ttgacagaag aacaaataaa
    14941 agaaaaagtt tatgaattag gaaaaaagat agaagaagac tttaaaggcg aagatttact
    15001 agtggtaggt atacttaaag gagctagtgt atttgtttca gatttaataa gatgtataga
    15061 tttggatgta aatatagatt ttatgagtgt tacaagttat ggaaactcaa cagagtcttc
    15121 tggaacagtt aaaatattaa aagatttaga tgtagatatt gaaggaaaga atgtgttgat
    15181 tgtagaggat ataatagatt ctggtttaac attaagtaat ctagttgcag ctttaaaaac
    15241 tagaaatcca aaatctttaa aactatgtac tctacttgac aaacctcaga gaagaaaagc
    15301 taatatacct gtagactatg ttggatttgt tatagaagac aaatttattg ttggttatgg
    15361 aatagactat gctgaaaagt atagaaattt accttatata ggtatagttg aggatgtaga
    15421 atagatttaa atgtgaatca aaagcactaa tttctaaggg agttagtgct ttttagttaa
    15481 ttatataaaa ttttagtaaa ggagacatct atatggatat tcagttaaat atattattat
    15541 ttttccaaaa tttaagaaat ccaattttga actttatatt tcttgtcttt acaataagca
    15601 cagaagcacc gttattaata ttaattacaa caattatata ctggtgtata aataaaaaat
    15661 gtggacagaa aatactgttt gctataattg gaaattttgt tgtaaattta ggcatcaaag
    15721 agtttgtaaa agctcccaga ccaataggaa taaaaggtct tgaatcattg agagtttcta
    15781 ctgctggtgg ttattctttt ccaagtgctc atactcaaac tgcaacaaca ttttgggttt
    15841 gtattatgac tatatttaaa aagagatatc ttcatttaat aggtgcagtt atggttttag
    15901 gagtaggatt gtctaggctg tatttagctg ttcattggcc gatagatgtg atagcgggtt
    15961 ggatattagg aatatttttt acagttgtat ttgtaaaaat atttgactat attgatgata
    16021 ataaaaatta tattttactt gtagttttac ttataccatt tataatagtg gctatatttc
    16081 taaatagccc tgaatatttt gaaaaatttg gtataatagt aggatttgta tttggatata
    16141 tggttgagga tagatttgtt aaatttaata cagataataa taacaaaaaa ataaatttta
    16201 gcaataaaaa taaggggagt aaaaatataa ttttttcaag aatatgtagg tttttggttg
    16261 ggatagtaac aataggaatg ttatatattg gcattaagtt gtttatttca atgttaatag
    16321 tcactttaaa tataagtgac tctactatga gtataatttt tatgaatttt attaagaata
    16381 cagtagttgt tttttatgga atagcaggag ctccagcatt atttaaactt ttaaaattaa
    16441 attgatttta aggtatagag tcaatagttt aaattaaaaa aattaagggt agaaaagagt
    16501 gattatttga agaaggtagt aataataggt ggtggaccag cagggatgat agccgcatca
    16561 actgcatgtg agaaaggcta tgatgtaaca ttaattgaga aaaatcataa attaggaaaa
    16621 aaacttgcaa taacaggaaa aggtagatgt aatattacta acgcttgtga aatagaggaa
    16681 ttaatagaaa atgtacctac aaatggaaaa tttctttata gtgcttttta tacttttaca
    16741 aatgatgatg taataagtat gtttaataat cttggagtaa agactaaaac agaaagagga
    16801 aaaagggttt ttccagagag tgataaagca tttgatattg taaatgctct tgaaagacaa
    16861 cttaaaagta agaaagtaaa tatattatta aattcgaaag tagaaaaaat aatatctaaa
    16921 aataataaaa ttgaaaaagt aattttaaac gataaaaaag aaattaagtg tgattcagtt
    16981 gtagtagcaa ctggtggatt aagttatcca cttactggtt ctactggtga tggatataaa
    17041 tttgcaattt cacaggggca cacaattata gatacaaaac catctttaat tggtatagaa
    17101 gtacaagaaa gttttacaaa agatttagaa aaattatcat tgagaaatgt tgaaataagg
    17161 gtgtttaata gcaaacaaaa gaaagtttat agtgattttg gagaacttga atttactaga
    17221 tttggtttag atggacctat aatcaagtct gcaagttgta gaatgaaaga tacaaggaaa
    17281 gaaaattaca ctatattatt agatttaaaa cctgcattag atgaggaaaa attggataaa
    17341 agagttcaaa aagattttca gaagtataca aataaaaaat ttgaaaaagc attagatgat
    17401 ttacttccaa aaaaaataat acctataata ataaacttaa gtgaaattaa tccaaatacg
    17461 gttgttcacc aaatttctag agagcagaga aaaaacttag tccatttatt aaaaaattta
    17521 aagtttacag taaagagata cagacctatt gaagaagcta taataacttc tggaggtgta
    17581 aaagtaaacg aaataaattc aagcacaatg gaatcaaaat tagtagaagg tttatttttt
    17641 gctggagaag taatagatat agatgcatat acaggtggat tcaatttgca aatagctttt
    17701 tctactggat atttggcagg ttttaattgc taaaaattac ctgaattaaa aatgtttaaa
    17761 atcacataat attatcagca acacaaaata aattaaacag aaggaaggta atcaacatgg
    17821 caagtaacaa taacaacaac agaacagtag taccaggagc aaaagaggct ttaaaccaaa
    17881 tgaaattaga aatagctaat gaacttggta tgagtaatta tcaacaagtt gataaaggaa
    17941 accttactgc aagagaaaat ggttatgtag gtggatacat gactaaaaaa ttagttgaaa
    18001 tggctgaaca acaaatggct ggaaaaagca acagatagtt ataaataatt atttataact
    18061 attggctatt agtttagcta gagctatctc aaatatagaa agtaatttct aaattgagat
    18121 agcttttttt tattaaatta ttaaatagat atatttttat gtaataaatt ttatgtattt
    18181 ggataaaata atacaaatga atatgttaca taaggagtga tgtaaaatga gtagtgtaaa
    18241 ttttgaggat ttaaaaaaca aatttattaa tagtgactta gatgaaaaaa taagaatata
    18301 tacaacaaca gaaggtttat cagtagaaca atttaaagag ctactaaaat attatccaat
    18361 acaacattta tctaaactag aaaaagcact tggatagcag tatttaatcc aaaattaaaa
    18421 aggcaattta tttaatatta aaaatatttt ttattaaata aattgccttt ttataatagg
    18481 tttaaattat tatacttttg atatataatt gtagattctc tcatttccaa gttcaataga
    18541 cccagtatta ataagagtaa ctattccttc tatatctcta gcagttaagt atttatatgg
    18601 aattaataaa ttataaccag tattacttag taagtttttt aattgggtta tttgagagat
    18661 attgaaacat tttgcagttt tagtagattt ttttcttaag ttagataaat tttcattttg
    18721 aatatttatt aaagtttcta tatagtttct ataagatatc ttaaagtttg tctcattgac
    18781 tattttttca ttaagtatac tactgataaa atcttgagac aatatattat ctgcaaattt
    18841 atctgctgtt aattttccag atgttatgcc tttttgatat tttctaagaa tttttttagt
    18901 atagcagtat attttatttc tcgtttctaa atcgagttgt tctatttgca caaaaccact
    18961 tccttctaat tataaaaata tttattaaaa atctatactt aactaataaa gcttcaataa
    19021 cttatttaca ataaatacta aatcataaaa tatttgttat tttttattat taccaagtat
    19081 aaaaaatatt ataattgtaa aacttacaaa tatatgtaaa atatttagga gtgatgaaaa
    19141 tgttaaatgg gataataaag cttttttatt ctgaaattgt aaaaacaaag attaataact
    19201 tagattcaag tggatattct ttaagagatg aatgtaaaga aatcagaaat ataactctaa
    19261 agatagatag tgtatttttt aagcttttaa agttagcttt tggttcaata ttgcttatgg
    19321 tagcttttaa tttaaacatg gttttaggta taggagtgtt tttagttggt gtagtttata
    19381 tactatataa aagaaatatg gaagaacaag taaaagagca tataaataat gtgaaaaata
    19441 atatcgacat ctctaaaata acttttatgg atgaaaaagg gagaacaggt ctaaatgcat
    19501 taattacatt acttcttata ggattattga caggatttaa ctggatatta gttgctagtt
    19561 ttgtcgttgt ttttgttttt actataaaga atgtatgtta aaatactaat aaatagttta
    19621 atatataggg ggagaaattg agtatgacta atggcagtta taaaatgaag aatttagtgg
    19681 aaatatccgc tatggtgact gaatctacag atttttttaa tataaaagat gacataatag
    19741 aaaaaatgtt agaagttgtt caccctacaa aggcatgtgt gaatctattt tataaaaatg
    19801 attctaaata tgcctactta gtatgttcgc aaaccttaga atatgtacct caaatttttc
    19861 caataaatag tcttaaaggt gcaaaaattg attttgacac atatccagag tatatacatg
    19921 aggctgtaaa tgaaaaaaag ataatatatg tggaaaatgt atttgaagat tctagagcag
    19981 aaggtgaacg agatttagca aaagctgaag gttatattgg gagaatagtt tttcctttta
    20041 taataaataa cgtagttgtt ggatttatga cttgtttttt aaaagaggat gattatataa
    20101 cagaacaaga tatagatttt atatcatctg ttgcatcact cttaagttta tcaatagaaa
    20161 taactaataa aaataataat gtacaaattt taattgataa actaagaggc tcaataagtg
    20221 ccataaatga agctactaaa aaactatacc ttaataagag tattgatggc tttttggaaa
    20281 atttaagtaa acaagcgtgt aatattacta atagtaaaga agctttaata attataaata
    20341 gtcatcataa taaggatgct atagttagtt gttataatgt tgagcaaaaa aagaaaacaa
    20401 atttatatcc tatgattgat atgtttttaa agaaagagtc attggggggg tatttaaata
    20461 atttaaaacc atgctcaaaa aagggaataa atttggaaag ttatatatat tacaaacttc
    20521 aggataaaaa tgagactata ggatgtatag tttgtgcaaa tagcaaaaac tatactagtg
    20581 atgatttaaa tattcttagt atattgtcca aacaagtttc tgttggtatg caattatatg
    20641 aacataatga aaaggaagta aaacacaaag ttcttgaaaa tgagttgaac atattaaata
    20701 aacaacaaca gcttattatg gataaaggta aaatggattg taatgatgag aaagaacttt
    20761 atttttacca taaaccagca agggtagtag gtggagattt ttattatgct acaaaagtgg
    20821 atgatgaaca tatagtttat attgttgctg atgtaatggg acatggaatg gtatcaaatt
    20881 atgttgttgc aatgataaaa ggtgctttta aagtattatg taatcaatac aatacaccaa
    20941 gagaaataat gacaaattta aataggatgt tatatgatga atttgacaag atgggagtat
    21001 ttacaacttg tttgataggt attatagata ctaaagaaaa tatgattact gtgtctaatg
    21061 caggtcacta tagtcctgta gttgtaaaga aagatggcac aatagaaaat aatttaaatt
    21121 gtaaaaaggg catacctatt ggaataatgg aagatgctac ttatgaaaat aatactcttt
    21181 caatggaaga ttatgcaatg gtatgtatgt acactgatgg aattttagag ataaaaaatt
    21241 catcaaaaga agagtatggt ataaaccgat tagaaaactt tctaaaagaa aattttagat
    21301 taaatcaaca gttaatagta gaaaatctaa aattggattt aaagaacttc tcatcaaaag
    21361 ataattatga tgatgatatt ttaatagtta tgttaaagga taggtagatt gattatggat
    21421 tcaaacgata aaaaaggact taaaagtcca gagattataa taataaatgg aagtccaaga
    21481 attaataaaa attgcagttt tatagcaaat gaagtaaaag ctatgctaga agagtgtgga
    21541 ttaagttgta aaatatttaa tatctacaat atggatattg aatattgcac agcatgtgga
    21601 ttttgtgaaa aaacagggta ttgcaagttt aatgatgata tgaagccatt atataaaatg
    21661 tttgatgatt caaaaggaac tatagtaata agtcctgtac attttgattc aatatcagct
    21721 aaattaaaga ctgttgttga tagaactcaa gctatatatg caagtaaata tgtattaaat
    21781 aaaccttcaa ttgatagaag taagaaacgg ataggaatgt atattgcagt gggagggagt
    21841 aaatcatatg attcacagtt taaaggtgga cagattgtta tggatttttt ctttaagagt
    21901 ataaatacta aacttttaca caatatatat ttaaacaata cagatgatgt atcttatata
    21961 gataatcaag aatttaaaga tataatatat aaaaatttaa agatatatat tgattcaata
    22021 taatttgatt ttgtaggtaa tattatgtat aattgtattg caataattta aataattaaa
    22081 aggagggaat ttcaatgaaa ttaccaatag cattatctaa taaacatgtt catttgagtc
    22141 aagctgatat agacgtatta tttggggcag gtcatgagtt aactaaattt aaaccattat
    22201 ctcaaccagg gcaatatgcc tgtgaagaaa aagtggattt agtaggacca aaaggaactt
    22261 taaaaggagt tagagtacta ggaccagcta gagctcatac tcaagttgaa atatctttag
    22321 cagatggttt cccattagga gtaaaagcac caataaaaga atctggtgat atagctggaa
    22381 ctccaggtgt taaattagta ggacctgctg gagaagttga aatgaaagaa ggagttatag
    22441 ttgcttctag acatatacat atgagcaatg aagatgctgc taaatttggt gtaactgata
    22501 agcaaatagt aaaagttaaa acatctggac caagagcatt agtatttgaa aatgtattag
    22561 taagagctag tgacagcttt aatctagaaa tgcatgttga tatggaagag ggaaatgcag
    22621 caggcgttaa aaatggagat ttagtagaat taatagttga ttaatattat aaatagactt
    22681 tatgctagta gatattttat ttgctagcat atttttaaat gtaataaatt tctataggat
    22741 aaatttaatc gattag
//
LOCUS       DAEORZ010000052        22608 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEORZ010000052 DAEORZ010000000
VERSION     DAEORZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.052"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            14..835
                     /locus_tag="KRH39_001866"
     CDS             14..835
                     /locus_tag="KRH39_001866"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBG8511771.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(911..2299)
                     /locus_tag="KRH39_001867"
     CDS             complement(911..2299)
                     /locus_tag="KRH39_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBG8511772.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLFLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMGEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVRNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(2434..3192)
                     /locus_tag="KRH39_001868"
     CDS             complement(2434..3192)
                     /locus_tag="KRH39_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG8511773.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(3194..3910)
                     /locus_tag="KRH39_001869"
     CDS             complement(3194..3910)
                     /locus_tag="KRH39_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG8511774.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLTNMFFT
                     IVGVAVFSTTEIWYIFPYSIPSRLMAVVLHILPNGLPVPEGSELLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(3912..4619)
                     /locus_tag="KRH39_001870"
     CDS             complement(3912..4619)
                     /locus_tag="KRH39_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG8511775.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGE"
     gene            complement(4735..5271)
                     /locus_tag="KRH39_001871"
     CDS             complement(4735..5271)
                     /locus_tag="KRH39_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG8511776.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(5373..6443)
                     /locus_tag="KRH39_001872"
     CDS             complement(5373..6443)
                     /locus_tag="KRH39_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG8511777.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSNMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(6436..7542)
                     /locus_tag="KRH39_001873"
     CDS             complement(6436..7542)
                     /locus_tag="KRH39_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBG8511778.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFTIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDLNKDINEDLNKDINENSYNNDYYNREEES
                     IKETDEYNDDSINFEAIYKDLEDIENKKGEDKHE"
     gene            complement(7529..7861)
                     /locus_tag="KRH39_001874"
     CDS             complement(7529..7861)
                     /locus_tag="KRH39_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG8511779.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            8060..9181
                     /locus_tag="KRH39_001875"
     CDS             8060..9181
                     /locus_tag="KRH39_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBG8511780.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(9309..11207)
                     /locus_tag="KRH39_001876"
     CDS             complement(9309..11207)
                     /locus_tag="KRH39_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBG8511781.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            11522..12298
                     /locus_tag="KRH39_001877"
     CDS             11522..12298
                     /locus_tag="KRH39_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG8511782.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(12390..13142)
                     /gene="xth"
                     /locus_tag="KRH39_001878"
     CDS             complement(12390..13142)
                     /gene="xth"
                     /locus_tag="KRH39_001878"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBG8511783.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADVFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEVLGSDHCPVELEIK"
     gene            complement(13295..14245)
                     /gene="galT"
                     /locus_tag="KRH39_001879"
     CDS             complement(13295..14245)
                     /gene="galT"
                     /locus_tag="KRH39_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBG8511784.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAYATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESNKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLNRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEELTKKLKID"
     gene            14630..15826
                     /locus_tag="KRH39_001880"
     CDS             14630..15826
                     /locus_tag="KRH39_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG8511785.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(15944..17278)
                     /locus_tag="KRH39_001881"
     CDS             complement(15944..17278)
                     /locus_tag="KRH39_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG8511786.1"
                     /translation="MKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVVEE
                     RQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKIPL
                     QFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALKRV
                     FPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDLVP
                     KIKALVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKVAK
                     SNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAHNK
                     QQIAIVITENNGAINNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAYEK
                     LAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIEVNKDYWPTLYKEN"
     gene            complement(17417..18169)
                     /locus_tag="KRH39_001882"
     CDS             complement(17417..18169)
                     /locus_tag="KRH39_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8511787.1"
                     /translation="MNLIDKLDINQKNLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(18317..19894)
                     /locus_tag="KRH39_001883"
     CDS             complement(18317..19894)
                     /locus_tag="KRH39_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG8511788.1"
                     /translation="MKQKMKGFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     IPFLANNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HASVNYMLGCRGILAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFA
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFISPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     MLGLVYTLVYYNVFKILILKFNLQTPGREEIVDVDFDLEPLSNKVEIAQSGETTNRAL
                     SIIKYLGGKDNIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDRDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            complement(20298..20750)
                     /locus_tag="KRH39_001884"
     CDS             complement(20298..20750)
                     /locus_tag="KRH39_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8511789.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITQE
                     GDKIAADFMDKLSDGLGEKLSKLTEEDRRKYLEAINTLQYILNKEEFR"
     gene            complement(21061..22506)
                     /locus_tag="KRH39_001885"
     CDS             complement(21061..22506)
                     /locus_tag="KRH39_001885"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBG8511790.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINKRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
BASE COUNT         7629 a         3715 c         2560 g         8704 t
ORIGIN      
        1 tggagggttt tttatgcaaa aaaaagtcgc agcaattaat gacttatctg gaataggaaa
       61 atgctcatta tcggttgcta tacctatact ttcagcatta aaggttcaat gttgtccatt
      121 tccaacggcc atactttcaa gtcaaactgg ttatcctgaa tttacttttt tagactttac
      181 tgatgaaatg gtaaaatatt caaatgtttg gaaaaattta aaagtcaatt ttgatagtat
      241 atatagtggt tttttgggtt caaagcatca aattgagata gtggctaatt ttataaacga
      301 ttatcccaat gcttttatcg tagttgaccc tgtgatgggt gataatggag ttatgtatcc
      361 aatattcaca gaagaaatga gacaggaaat taaagaatta gttaaacatt cagatttaac
      421 tactcctaat ttaacagaag cttgtttctt aactggaaat gattatacca aaagcgatta
      481 taatagagat gagcttatat atattgcaaa atctgtatct gatttgggac ctagcaaagt
      541 cgttattact ggtatattag aagatgataa tatattaaat ttggcttacg atagagataa
      601 tgaccatgta ttctttacat cagtcaaata taataattgt tcttacagtg gaactggaga
      661 tatattcaca tctatacttt gtggtatgtt agtaaataaa catgatttgg gagtagcggt
      721 taatactgca acagatttta tatataaaac tataaattac acatctcaat ttgatactga
      781 tagaaatgat ggagttatgt ttgaaaactt tttatcagat ttaaccaata tataaaatca
      841 aaaaaactat ccattttata aatacctata aatcataaaa tttaggtatt tacaaaatgg
      901 atattattta ctaaactatt ggtatttcaa ttgttaccat agcaccacca gttttcacag
      961 aattttcaat acataaagtc ccattatgtt tttgtacaat attatttaca atatagagtc
     1021 ccattccata atgagattta gaatttctac tcaaatctcc tgtataaaac tgttctgtag
     1081 cttttgacaa atctgcttta gaaaaaccac ttccacaatc tgtaactaca aattttaagt
     1141 atttattacg tactgataca cttatgtata gtttactctg gcttggtgaa taatctatag
     1201 catttgaaat tatattcata atagctctga ataacagttc tctatctata gttatatttt
     1261 taggcaaatt atcttgttta taaaactcca catctaagtt tttagtacta gctaatgctt
     1321 ctatttgcac taagacatct tttataaact cacccatact gattgcttct ttatttaata
     1381 tatagccatt ctctgttttt gatatatcta ttaaagtatt aatatatttt tctatttgag
     1441 tgacaccctt tgagatatat tccatgtatt ctaataactc aggattttca tttatttcaa
     1501 ttaataagtc agtatttcca tgtataattg ttagtggtgt ttttatatca tgtgccagtg
     1561 cagatatctg ttcctttcta ttttcttcta gcatccattg tttttctaaa gaatctttta
     1621 acgccaactt cattttatcc atagaaaaaa ggacattatt aatttcataa attttactag
     1681 attctacaac aaaatctaaa tcctgttgtt ctattttttc tgtagtattt tttaaaagct
     1741 ccatttcaat attaaatttc ttgccaaata cttttgataa tataacaatt tcaataaaaa
     1801 atattatact aaaaataatc atacccaatg tctctggttt tggtaaatat gctcttaaaa
     1861 ttggagaact atactctgat actattttgt attttattat aaaaacttca tctttcttaa
     1921 aaaactttat ataactttct gaatatgcat gcctttcttc aatatctctc attaaattcc
     1981 atacttcttt tgattctttt ttattaaagg tacctgaaat taaattccca tctaaagtat
     2041 aaaccccata ctcatatata cttggaatta tatcttcagt tactttttca cttgaaataa
     2101 tctgttcttt atatttatta aattgttttt ctgcataatt agctggtaaa attacacctg
     2161 atgataataa tacagaaaat gataacatta aaaagagtac aattgaaatt gtcataataa
     2221 agaataaaga taaatatttt ataaaaatag ttcttaactt ttcaactttt ttgttgttag
     2281 attttttacc tccaagcatt tgtaccctgt cctccaatag tttaattaat tcataattaa
     2341 tacccaacca gttttttatt tgattatttt atatcttttt attgctaagt tatattttaa
     2401 cataataata taaataaaca gtcaattttt actctattct tctgattttt taccttccca
     2461 tttagaaaac caaatacata aaattacaaa tgctaaaatt gttacaaaac cacaaattaa
     2521 acctgttgat tgatatgctt ttattgattc aacctttata tattcaaccg ttttagaact
     2581 tgaaaaagac gaccaaatag ttataaatct aacaccccat gcacaaggaa aatactgcca
     2641 aataccagaa cccaatccag ttaataataa tgctgaaagt aacatttcaa ctatacctac
     2701 tccaattgat gttccttttc cgaaccttaa acttaagaat aaatgtaata tgtattcaaa
     2761 taaattacac ccaattaaaa ttagaattga aatcatataa aagcttgatg taaagtaagc
     2821 cttagatata ccaaatttaa aacctaatac agacactaat gttgctacaa aaccaagaca
     2881 tactaagatt atatatttac ttataagagt aagatatttt ggattagatg ttgttaacat
     2941 atgtttataa ttccctgcat aatattcttg ttcaacaaac attgaacaca caacacttga
     3001 caataaagga aaagctattg ctataacttg taaatatgct actatcttta gtatattatc
     3061 agctcctgaa accttataat accataaaaa caatcccatc cccattaaag caatgcaaat
     3121 gtgcattagt attataaaat ttctctttaa ttttattaaa tcagaacaaa atattcttat
     3181 taactgtctc atattacttc acctctctat tattgaacca aacccttgta gctaaagcaa
     3241 ataatacaaa taaaacaata gatattaata ctcctggcaa aataacagca ttatttaaaa
     3301 gttcacttcc ctctggtaca ggaagaccat ttggcagaat atgcaataca acagccatta
     3361 atcttgaagg aatcgaatat ggaaatatat accatatttc tgtagttgag aaaacagcaa
     3421 ctccaacaat agtaaagaac atatttgtta gaactgtaac aaatatacca aattttgaag
     3481 ctaaaaacat acaaagagga atctgccaca taaatagtaa aataattaat atagttgcaa
     3541 tgatattttg acctattgaa atagtttctt tatagattat cccaacaaaa actacaccta
     3601 ttgcagaaat aattgatgct ataaaacaac aaataataca gtaaatcatt tttccagtcc
     3661 aaacgtcttt catttcaatt ggcaatggta aaataccacg atatttactt ttttcatctt
     3721 ttaatactat taaagaagat attaatgtta atgctcctgg aagaaatgtc gtataccacc
     3781 agttaaaagc agcattttgc cctccaccta gtccacacaa aagtatacaa aacactggaa
     3841 ctatccatat caacttcatt ccaaaagttc ctttcatctt ttgaaattcc actctaataa
     3901 atctttccat attactcccc tctctttcta ttttcagcta caactttcat aaaaagttct
     3961 tctaagtttt gttcttttga tacttctcct tgataaccca ttactccatt agaaattatt
     4021 cctatttcat ctacaacttg ttcaacttca gacaagatat ggcttgataa aataactgta
     4081 attccttgtt ttggaaaaga acgaatcaat tctctaagtt cttcaatacc aatagggtca
     4141 agaccatttg taggctcatc taagattaaa agttttgggt gatttaataa tgcaatagca
     4201 ataccaaggc gttgcttcat gcctagagag aattgacctg aacgcttttt aaaggtatct
     4261 cttaaatcta cagtctctaa aacttcttca atgcgagagt caggaagacc aagtaaagta
     4321 gtatggactt ttaaattttc tcgtgctgtt aaattctcat atatagcagg agactcaata
     4381 agagaaccaa tatttgataa atcctttcta gtccatctat gtccctcaaa cattattttt
     4441 cctgaagttg gatttaaaat cccagttatc atttttaata aagtagattt cccagctcca
     4501 tttggtccta ataatccata tatagaatta gatttaattg ctagtgaaat gttatcaact
     4561 gccatctgct ccttaaattt tttagaaact ccttttacct gcaaaatata attactcata
     4621 attttcatcc tttcttttgt caaacttatt tacaagaatc attctaaatc ataaatataa
     4681 ggattttata agaattaaaa aacaaaaaaa gatgtgttaa aaacacatct ttttttatct
     4741 taattctatt aattttccat tgtagtatat ctctacttct tcaatccaaa tgctattatc
     4801 ttcttcttgg atgacatttt taataatatt atttatagta tatattatat tttgttcatt
     4861 caaataatca tctttccaag aaactctact ttttttatct ggttctatca agtttatttt
     4921 aacaagatta tctttttcct taacttctac ttccatgtct aaaccattaa aatactcatt
     4981 tgataatgta tctaagatta atcgcagttt atccttaagt gaactttctt catcaatctg
     5041 taagtacttt actattttct catcctttga aattatctct ataggtattt tagtagtttt
     5101 tttagaatta atcattttac tagttgtttc ttcttttgca tcttcatcct tctttacaac
     5161 tgctgattct ttagaatcaa ctagctcaac tgcactgatg ctataatcca tacatgccgt
     5221 actcaataaa gtagtagcaa ttattaaaaa acttactgct attttattca taagtatccc
     5281 tctcctaatt ctctttcgat acttattata ccatagtata gattatattt ttatactttt
     5341 aaatcaattt ataaattatt atagtaaatt atttacaatt ctaagctatt tgaatttatt
     5401 tttatagtat attctttctc taaattcgac agatttttat tcaaaagccc ttttatttcc
     5461 ctcgtatttc tatacttgtt gctatgttct tcactatgtt cataatcata tgaatcattg
     5521 tcatattctt catcattatt aaattcttca attgcttctt ttgactttat atcacaatta
     5581 aatttatact tatttactgg tatatcaagt cttaccttag aatttggaac ttctatgtcc
     5641 aggaacttag caattggcat attagagtac aagtaaaatt cactttcata atttgaattt
     5701 ctacctattt tctcttttat atttacattt tcaggtatat actcttctat attattgata
     5761 aatacatctt cattctctga agatatatac aatgactctg attctatgtt tacattttta
     5821 acacctaaaa ttgattttgt tgacaagtga agattgttat tagatattaa gtttaggttt
     5881 tctaatctat ttactttgtt ctctgttata gctctactaa aacctccata agaagtttta
     5941 tatgttatag tatttgaagc tacattatcg tatacaaata agtgtgagct tatacttgat
     6001 gcatttatgt ctacattatt tggtacatat attataattt ctctataatc atgtgaaaac
     6061 atactattta tagatgtatt taataattgg tcgaaatttt taatcttttt aattttattt
     6121 tcatattttc tttcaccttt tacaactaaa gtcttatctt ttaaagttac attgtatttg
     6181 taaaactcat tatttcctct tgttgttaca cgcacatctt cacttgaact tttctttatt
     6241 gtaacgttta tattgtcaac agctatatct aacttgtcaa tatttacttt ttcttgataa
     6301 agtttatttt cttttttaaa ttcagcttcc ttttctaaac caaagtttat taggcttggc
     6361 ataactaaaa tccctgacca tgttgtccct attataccta ttaccaataa aactgcagca
     6421 aatatagcaa gttttttatt catgtttatc ttctcctttc ttattttcta tatcttcaag
     6481 gtccttatat atcgcctcaa aatttatact atcatcatta tattcatcag tttcttttat
     6541 gctttcttct tctctattgt agtaatcatt attatatgaa ttttcattaa tgtctttatt
     6601 caaatcttca ttaatgtctt tattcaaatc ttcattaatg tctttattca aatcttcatc
     6661 atttaaagta gctttttttc ttttttccat cttttcattt gcctttatat aaatattttt
     6721 tctctttatc caatgtatat aattatttag taattgttta cctagtttta tggtaaataa
     6781 taaaatttgc catgcaagta tctcaagccc tacaaatgct atggttgcga aaatacaaaa
     6841 catcataaca tctggaacca ttgctataaa tttaatcaca aatggaatta atataacaaa
     6901 agcaattaat gagcatacta atgcaatagt agcagataca atagcacata caaacgcaaa
     6961 tgctggaaca attagaaata aagataacac tactaataac ccctttataa cttgtctgga
     7021 taatcctcct acgccttctt ctatacttgg tgtaagttta tcatttatat tttcttttga
     7081 tttattgtac ttttccttag cttcatcttt caagatagag taatcctttt ttacatttga
     7141 tttaaatcta ctaaatttac catttaatat gtctgtattc attatttttc catctatatt
     7201 ttcttttgat ttactatact tttcttttgc ttcatctttt aagatagagt aacctttttt
     7261 catatctgat ttaaatttac taaacttatc attcaaaaca tctgtattta ttattttttc
     7321 ctcttcaata tttttttctt tagtttgtaa gataagttct tttgctatag attttggtga
     7381 gccaagtgat gctattatct ccatatcact ctttccctct ataagaccat ctacgaaata
     7441 ctcttcataa tctctcaata tatcattaac ttcgctctca gaaaaatctt tttttagata
     7501 atcctttaaa atttcaagaa actcagtttt attcaagttc attacctccc tcatcttcac
     7561 ttattaaaat ctcaacagcc tttacaaatt ctctccactc ttccatcaga ttggataaat
     7621 tctcttttcc tagtgaagtt attctatagt attttctagc aggaccttca ttagattcta
     7681 gtatatatgt ttcgaaatac aaatcttttg taagtcttct tagaatagga tatattgttc
     7741 cttcatttac atctattact ttagagatgt tatgtactat ctcatatcca tacatatctt
     7801 tcttagatat gagagcaagt acacaaattt ctaaaacacc ctttctaaat tgtgtgttca
     7861 tatactccat cctttcttta aatacacagt attgtgtttt acaaggtact atgttaaaac
     7921 ttcttatatc ctcataataa cacagtacta tgttctgcac aatagttatt tttaaatatt
     7981 cttgttgatt aaatccttct ataaatgtat aatactctaa tcagatatat tttatgaaag
     8041 tggtgataaa ttggctaaaa tgaaagatga taaaacaata ttaatcctaa ctgctcaatt
     8101 tggtgctgga catataagtg ctgcaaaagc agtcaaagaa tgtattattg aaaaatatag
     8161 taactataat gttgttatac aaaactttat aaatgcaagt attcccatga tgaataagcc
     8221 tatggtaaaa ctttatgaaa acaatacaaa atatactcct ggattatata attactatta
     8281 ttactttaag aaatcgtttg attcaagaca tgatttatct cataaattat atacacctaa
     8341 actttctgag tatattgctg atataaatcc agatttgatt atctctacat ttccactagc
     8401 tgctgcttgt gtaaataact ttaagataaa aaatcctgat ataaatatac ctacacttac
     8461 agttataaca gatgttgtag atagtatgga atgggttttt gaaaatacag atttgtattt
     8521 tgttccttct cctgaaataa aaaatagatt ttttcagaaa ggaataaatc ctgactccat
     8581 aaaggttact ggagttcctg ttgataagag atttcaaatt gaaagtaaag aactatgttg
     8641 tgataaatat agactattac ttctaggtgg aggaagaggt ctatttgata tagatgagga
     8701 ttttatgcat tggatagatg aatttattga agaacatagt aattctatag aaattacaat
     8761 agttactggt aaaaacaaaa aattatatga caatctaact cataaaaaac ctctaaaaaa
     8821 tattaaagtt cttggatttg taaacgacat gtataatcta ataagagaat gtgacctaat
     8881 gctaactaag cctggaggag caactatatt tgaagctatt caatcacaaa ctccagtact
     8941 cgtaaaaatg ccaaaagttg gacaagaaat tgaaaacgct aaatttataa tagataaggg
     9001 tcttggcatg atttatagtg atgacctaga ccttaaaaac atattttata aattagtatc
     9061 aaatgaattt gattctataa taaactttat gaaaaaaaat ttagaagaat ttaaaacagt
     9121 cattcatcca gagaaaattg ctgattatat atcagaatta atagataatc attacagtta
     9181 aattgtacta ttaaaaaatt tcaaacataa aattaaaggc aatcaagtat gcttttacac
     9241 ttgagaattt actcatagtg tttaataata ctcgattgcc tttatcatat caaaatcaaa
     9301 ttgttatatt agtatatatt tttcttgtac tgcatataca gttgttgtat ttcttccata
     9361 cttctcttta attctatttg taaatctgaa gcagtctggt aatctttatt ttctatagct
     9421 tctctcactc tagtgaatag tgattctcta gttaaagtat ccttcataat attgaatttt
     9481 aaatccgata ttgagttcca aactacttca tcaagcgcat ccatattttc ttttgtgtgt
     9541 atcttgacaa tacttcttat tttttgaatc ccagcctcta ttattctagt ttttaatttc
     9601 ttttgccact tatctatagc tccaatctta aatgattcta ttatactatc agtaaataca
     9661 tcaccttgtt ttaaagattt taattttgaa gcatatattt ctaagttttt catattttca
     9721 taaactgtag ctggtggtat tccaaatctt gcatttcttt cttccaaact ataatgttca
     9781 aatacatcat tttcatctct atatgctcta tctttttcta aatagaaact ttcttcacct
     9841 acatttttag ataactcttt ttcaagttct tttgtactaa gaccactctt agctgctgct
     9901 tttataccat ccaacattgt ttgataacat cctgctatta ctaaatacgt gttagataga
     9961 gggcttggtg accttaactc aaatcttaca gtctttggat ttttcatatc tcttattaat
    10021 ccaacaagca ctgacctatt tcttgatgga atctcataac tatgccctaa agatgtaact
    10081 atgcatacag gagcttcaaa gccaggaact aatctattaa atccatcatt agaagctgtt
    10141 acaattgggt ttaatacttc gtaattgtaa agaagtccca taagagcccc atatcccagt
    10201 tcacttagat aatcttcttt taaatctttt ggagcaaata aattttttat acttccatcc
    10261 tttaacttag cacttactcc tacatgtgta tgtccaccac ttcctgctac tccatgaatt
    10321 ggctttgctt taaatgtaac ttctagtcca tgtgatgtaa atacatcctc tataacatct
    10381 cttactaata attcattgtc tgctgcttgt aatggtgtag aaaacttcca agaaacctct
    10441 aattgctcca tagcatggtt tgttcttcca tctatactaa tagaactttg tattcctccg
    10501 acttctttgt gagccatttc agggtttaca cctagttttt gaagtataat taaacttttc
    10561 tccaaacaag ttcttataat accatgagtt cttttccagt attgctcttt taggctttgt
    10621 gatacatata atttctctag gtcagcttta tcctcaggag tattaaccca gaactcaagc
    10681 tctgtagctg ctgttaacat tatttcctct atatcatcta ctgaatctat tcctatgtta
    10741 tttattacat gtggatattc tctaaatatt tcatacattg actttttaaa gtacttatct
    10801 gctttttgaa gtactcctct agagcatact tttttattat catgtattaa aaatgcaggt
    10861 atctttaatg ttccaacagg tagacctact tcttcgtcta tatattccat attgtaatct
    10921 acataccaat gacaactttt atctggcatt aaatctactt ttgcattatt taatgtagct
    10981 atgttataaa gctcaacact tgaaccatca gtctgtatag ctgattccaa aaatttatct
    11041 atatcctcta aaaataattc tacaggaatc ttttcatctg ttgcatttcc tcctaaatca
    11101 acccccatta aggaaacaaa tcttatattt ttattgcttt ttaggatttc tcttaattct
    11161 tcattagtgt gtttatcttt ctctattacg tataatagac tatccatttt tccactctcc
    11221 cataattttt atgtatattt tattttgtta aatacaagat aaatttcatt ttccttacat
    11281 ttatgaattt ttcttgcaaa tcattcgtgt tttattcgtg tttttattta tattattata
    11341 tatcaagtac aaaaagttcg caatattttt tattggaaaa aatattatac atacaatagt
    11401 ttaacatttt tacaaatatg gggtatacta aaataaaagt atttttattt gaaaatttaa
    11461 ttttatttat aatataatct ataataaaag aaataataaa aaaatttagg agataaactt
    11521 tatggaatac ataattttcg atttggaatt taaccaagga tttgataaaa aactcaataa
    11581 aactgtatcc aatgaaaagt gtccttttga aattatacaa ataggagcaa ttaaattgga
    11641 ttctaaattc aatataatag atacgtttaa tagctatgta aaacctacaa tttataaaga
    11701 catacatccc tttattagtc gtatgactaa tattaaaaat tctgatttta atgattcgcc
    11761 tactttccct gaggtctaca acaattttat aaaatttata tcatcacaag accctatatt
    11821 atgtgtttgg ggagctggag atttaaagga attatacagg aatatcaact accacaagct
    11881 accatcaaat tccttaccta aatcttatat taatatacaa caacatgcat ctaaatattt
    11941 caataatcca gcaggaaaaa gcattggctt acaaaatgct atttctatat tggaacttga
    12001 tgaaaaaatg tcttatcaca atgctttaaa tgatgcatat tatactgcta aagtttttat
    12061 taagatatac aatccaagta tagtcccaga tatatatctt tatacgagca tcaaacctaa
    12121 aactataaga tattctaata aaaaaagagt tgattatgat aagttatttg atgaatttag
    12181 aaaaatttta aatcgtgaat taactaaaga cgagaaaaaa atcattaact tagcttataa
    12241 tatggggaaa acaaatcaat ttacattaga aaatgttaag caaagaaaaa acaaatagag
    12301 tttatttaga taaaaaatag gcatgtagat taaaaaaata gttaacttta aatttatatc
    12361 tacagcccaa tttttatcgt aattaatttt tattttatct ctaattcaac tggacaatgg
    12421 tctgaaccta atacttcagt gtgtatatcc gcacttacca atctatcttc taattttttg
    12481 gatgcacaaa aataatctat tctccaccct gcattatttt ttcttgcatt aaatctatat
    12541 gaccaccaac tatagacacc ttctttatca ggattaaaat atctatatgt gtctataaat
    12601 ccactgtcta ataatttaga aaacttctct ctttcttctt tagtaaaacc tgcatttttc
    12661 atattatttt taggattttt taaatctatt tctttatgtg caacgttcat atctccacac
    12721 acaattacag gcttatgatt atctagtttg gttaaataat ctataaagtc gtcttcccat
    12781 ctagttctat attctagtct ctttaactca gattgagaat ttggcgtata tactgtaaca
    12841 aagtaaaaat cttcaaactc tagagttatc actctaccct ctttgtcatg ttcttctata
    12901 tttattccat acattacttt taaaggctct ttttttgtaa atatagcagt acctgaataa
    12961 ccctttctct cagcataatt ccaatattga aaatatcctg gtaaatctaa ctctatttgc
    13021 ccttcttgca atttagtttc ttgtaaacaa aatacatctg catctacttc tttgaaaaag
    13081 tctaaaaatc ctttcccaac acaagctctt attccattaa cattccatga tataaatttc
    13141 atacttatac tcctcatcta aacattgata cactccccat atctaaagct gggggctttt
    13201 caacaagttt ataaattctt aatttacttt tattttaaca atactataat ttcatctaaa
    13261 attactattt acttaatacc taaaaatata tgttttaatc aattttcaac ttttttgtta
    13321 actcttcagg gctcattgaa gaaacataca tatttgttcc aagttcaaat cctccaaaat
    13381 tatattttct tgggattatg tgcatatgaa cattaaaata cgattttgtt tcaatctttg
    13441 ttggatgagt gtgaatacat aagttaaatg gcatatatcc atttatattg tataacttct
    13501 taaacaactt ttcaaatatt gtagaaagtt cctgtatatt attatcattt aattcaccaa
    13561 actctatttt atctttaaat attattctta cttctccact atacttggtt gcatacggta
    13621 taagtaccaa aaaagcttct ccattataaa caactcttct atttaaactt atttcatttt
    13681 ctattaaatc atcatacaaa gaacttttat tgctgtcata atactccttt gaaaccaaca
    13741 gttcattcgt aatttcaggt ggaataaaag acatagacaa tatttgagca tgtggatgca
    13801 ttaaagatgc tcctgccttt ctcaagaagt ttttgaagat actaacatac tctactttat
    13861 cgtctttcga caaatctcta aatctatttc tatacataaa aaaaacatct tcaaattctt
    13921 tttgactcat gttataaaaa ctacgattat gtttattgct ctctatcatt acttcatgaa
    13981 caccatatac atctcttgcc atatcatcaa ttataggaaa tttattgtag acagatttta
    14041 caagccatcc ttcttcactt tctatttcaa ataatgcatc agttgcataa gcttcatttc
    14101 ctttgcaaaa tgggcattct tcatcatatt cattagtttc ttcatcttca tttgggatat
    14161 ctgctgtatc taaaggtctt tttaacctat ccgtagcaaa tataacaaca tcattggtaa
    14221 ttggatctat tcttaattct ttcataatta acacctctta gttaatagcc tttaatataa
    14281 ttatatgata tttatcgtat ctgttaaata aatttataat attttttata attttgcata
    14341 tttattatta tatattttgt ctatagctga aagattataa ctaaaaaatc actatcagta
    14401 tatcagaata ttatcttact ataaattaat ttatgaatac ataatttagt agcataaaca
    14461 tgggtatttt actataattt gttaaaagtt tattttaaag ctatattaat tttttttgat
    14521 gatttttacc cataagtatg ttattattat cttgtaaaga tgtcttgtat tcaaacatac
    14581 aatttacata atacaatata cgtatttaaa ttaaataggg ggtattaaaa tgaattattc
    14641 aaacagagtt agtgcaatgc aagcctctcc aatcagaaaa cttgttccat ttgcacaagc
    14701 agcaaaagat aaaggaatca aagtatatca cttaaacata ggacaaccag acataaaaac
    14761 tcctaaagga ttttttgatg ctgttaaaaa ctttgatagc gaagtcttag aatatgctac
    14821 atcagaaggt attccagaat tattagaagc acttcaaaat tactataaaa cttacgatat
    14881 gaattttgaa aaagatgaat tattagtaac taatggtggt agtgaagctt tacttttcac
    14941 tatgatggca gtttgtgacc caggagataa tctattagtt ccagagccat tctatacaaa
    15001 ctacaatgga tttggtcaat ctgtaaacgt agaagttaat gctgttacaa caaaggcaga
    15061 aaacggtttc caccttccat ctaaagaaga aatattgtct aaagtagatg ataaaactaa
    15121 ggctataatt ctttctaacc caggaaatcc aactggtgca atttatacaa aagaagaact
    15181 taatatccta gctgaaatag ctaaagaaaa agatttatgg ataatagcag acgaagttta
    15241 tagagaattt gtttatgatg ggcttgagta tacaagctgt ggtaacttag agggtgtaca
    15301 agatagagtt ataataatag acagtgtttc taagagatac agtgcttgtg gtgctagaat
    15361 tggttctatc gcttgtaaaa ataaaggtct tatcgctcaa atacttaaat tatgtcaagg
    15421 aagactttgt gttcctacat tagaacaaat aggagctgtt gagttataca aaactcctgt
    15481 ttcttacttc aaagaagtta atgaagagta caaaaaaaga agagatgtat tatacaatga
    15541 attaatgaag gttgaaggtg ttatatgtaa aaaaccaact ggtgctttct atatagttgc
    15601 taaattacca gttgaaaatg cagaagattt cacaatttgg atgttaaaag aatttaacaa
    15661 agataatgaa acagttatgg tttgccctgc tgaaggattc tatgctactc caggtttagg
    15721 acgtgatgaa ataagattag cttacatcct aaatgaaaaa gaccttcata gagctgctac
    15781 attattaaaa gaaggtttag agcaatatgt agctttacaa aaataatttt ttaaacttaa
    15841 tttatataaa tttaatgata taaaatgtcc ccttttcaga tattatttga aaatgggaca
    15901 tttcttagtt aatatattaa ttaagactta tacctgactc tttttaattt tctttatata
    15961 gtgttggcca gtaatcttta ttcacttcaa ttaaagcatc taaaacttgt cttgccttag
    16021 ttggagaaat tatagttcta tttaatgtta aagataaaat agctttttca taagattgtt
    16081 caaaatacgc atcaactgct aacttttcat aagctaattg accttctaac agtcccttat
    16141 aaaatgttgg tatattacct acatgtaaag gtctcggtcc attctttgta agaatcgcag
    16201 ctacttctac cattgcatca tcaggtaagt tattgatagc cccattattt tctgttataa
    16261 ctatagctat ttgctgtttg ttatgagcta ttgcttctgc aacttcaact ataaattccc
    16321 catgagcatc attatgcact aaattagaac ctatagatgt tcctttttct ataacagatt
    16381 tacattcttc aaatacacgc ttttctcttc cattcataac ttctcttgct ctagtacaat
    16441 ttatatttga tttagctact tttttatcag gatatagata atactgtaca tatgtattag
    16501 gtaagtaatc tggtacatct tccaacatat ctttaaccat tccataagta tctagccaag
    16561 atttatctct ttgttctgca tcaacaggtt ggaatccaga gtttgctact aaagctttta
    16621 tcttaggtac caaatcttca ccatttttat catacaaatg tgtaaaccat ccgaagtgat
    16681 ttaaaccaaa gtatacaggc tcaaatgttc ttgaatccat atctaacaat cttccataag
    16741 accttaacaa gttaactggt tggtcacaca tattaagcaa tcttttatca tctggaaata
    16801 ctctttttaa tgcttctgca acaatagcag caggatttgt ataatttaat atccatgctt
    16861 taggagagta ttttcttaca gtcttaacca tttctatcat atcacctata gaccttaacc
    16921 cataagcaaa tccaccagct ccacaagttt cttgaccaac caatccaaat tgtagaggaa
    16981 tcttttcatc taattctcgc atcttaagac caccagttct catttgaaca aatacaaaat
    17041 ctacatctat aaatgcttca tccgggtttg tggtatattc aagtgttgct ttaggatatt
    17101 cttctttaaa taatattttt ccaaattccc ctattaatct ttgacgttct tctactacat
    17161 ctaacatagt tactttattt agaggaaatc tatcactcat tttacacaaa gactttaaaa
    17221 gtcctggagt ccaagtactg cctcctccaa ctatagctat attgtatctt tttttcattg
    17281 tcatactaat cacccctgtt ttttattaac taacttgtaa gatgattata atatataaag
    17341 ttttctatat gaatactttt aggtaatagt taaaaatagt tcaattttag tatttttttc
    17401 aaaattgtaa tttttttcat ctattgtatt tttcaatgta tttatgaaac atatattcca
    17461 taagagaaag tgctggcact ctagatgtat agttaacact attaaagtta tattgtggag
    17521 aagatacaaa taattttata tcagctatat ttgataatga gtttacactt atatttgtca
    17581 aagaaattac tttaacttga tttgtttttg ctatattaca tgccttgata acctgagcag
    17641 tctctccaga catagataca gcaattacaa ggtcatcttt agtaatattt gatgcagcca
    17701 aatacatgag agttgagtca tcatagtatt ttacatttac acctatagct gaaaaacgtt
    17761 gagacatttc agtacatact aatctagaag acccaacacc aaaaaaatat atatgcttag
    17821 aattatgtat ttcatttaaa cacatatcta tattctctac atttattaaa ttctgtgtat
    17881 ccataacatc agtaaaaata tcactttgac ttttagtaaa ctccttattt ctttcatttc
    17941 tatttgaata tgaaaaaact aaaaagtttt taaattcact atatccagaa aagccgagtt
    18001 tctgacaaaa ccttactatg tatgcagtcg aattaaaagt ttcatttgct atatctttaa
    18061 tctttttttg atttattttt tcagaatttt ttagaaaata gtttaaaaga gatatttcat
    18121 ttgtagataa atttttttga tttatatcta atttgtctat taagttcatt tatacctcca
    18181 taataatgat ttgtagttaa acaacttcct catatttatt atataaatat acactataca
    18241 gcatttatct tatgtttatt atactacaaa aaagacaata caaattataa attagtattg
    18301 ccttttaata tttactttaa atagacattg caaaagaaac ttcgtctctt acttgagata
    18361 cagatggtcc ataaattatt tggatatctc tatcagatac ttttataact cccattgcac
    18421 ctgtatattt tttaaaagca ttttcatcta ctttactcat atctttaact actactctta
    18481 agcgagttat acagtttgtt atatcttcta tattgtcctt accacctaaa tatttaatta
    18541 tggataatgc tctattcgta gtttctccac tttgagctat ttctacttta tttgataaag
    18601 gttctaaatc aaaatctaca tccactattt cctctcgccc tggagtctgc aagttaaatt
    18661 taagtatcaa aattttaaat acattataat aaactagtgt ataaactaaa ccaagcatta
    18721 ttgaataaat ccactttgta ttaggttgca taactccaaa tactactaaa tctattatac
    18781 caccttgagt atttcctatt gctactccaa atatatgcat catcataaat gatatacctg
    18841 ataaaaatgc atgtattaca aataatattg gcgatataaa taaaaaagaa aattccaatg
    18901 gctctgtaat tcccgttaca aatgttgcca aagcaccagc tagtaatatc cctttaacct
    18961 tcgacctatt ttcaggcttt gcagttttat acatggcata tgctgctgca ggaagaccaa
    19021 ataccattat aggtattttc ccttgagcta aaaatctagt tgcttctgtt gaaaaactga
    19081 ttgtttcagg gtcacttagt tgcgctaaga atatgttcaa tgctccaaat acttcttgcc
    19141 ctcctacaac taaagaacct cctacttcag taaatctaaa catagcattt aatatatgat
    19201 gtagtccaaa tggtattaaa agtctttcaa gaaatccaaa tgcaaatgtt cctaaatatc
    19261 ctaaactagc tataccaata cctacactct gaataattgt atttacatat ggccatatat
    19321 atggtattat tatccccaaa atagagaata ctactgttgt tattattgga acaaacctag
    19381 ttccaccaaa aaatcctaaa taatctggca gttttatatt gtagtattta ttatgtaatg
    19441 atgctgttat tataccaact acaattccac caaatactcc taattctaat gtttgtatgc
    19501 ctaaagttac agcttgaccc atttctctca tcatgtcagg ttcagcaaga atacctctac
    19561 accctaacat ataatttaca ctagcatgta atactaaata accaacaaaa ccagaaaatg
    19621 cagaaactgc tttttcttct ttagcaagac caacagatat tgatatagca aagaatattg
    19681 ctaagtttcc aaaaagtact cctgttatag cttttgtaaa acccaatatt ccttgaatga
    19741 aattatttgc taaaaaaggt atatcactaa ctatattagg atttgaaaat gctgaactta
    19801 ttcctaaaaa tatacctgct gcagatatta atgctattgg caataaaaat gattttccta
    19861 actgcaaaaa gaaccctttc attttttgtt tcatttctat ccctccattt ttaaattttt
    19921 aaaacgtttt ctatgtcata attatatatt tttattttgt ttttgaaact ttataatttg
    19981 ttttaaaata tttttacata aatagtaact ttttcaaaac tgtaaatttt ttcaaacaaa
    20041 gacatagtaa cgttgtaaca tatataaatt taaaaagaga ttagaaaata aaatcattga
    20101 atataaaata gaaaaagata gatttcgcaa atagatatga gtaggtactg acaaatactg
    20161 aaatttataa gaaattataa aaaagtacat cgaaaaaatt taagatgtac tttttagatt
    20221 gtatttagat ttataattat atagctaaat gtataacaaa gaattaatct taaaactatt
    20281 aaaatttttg tatttttcta tctaaattct tctttgttca atatatattg aagagtattt
    20341 attgcctcta aatattttct tctatcttcc tcagtcaatt tagaaagctt ttctcctaaa
    20401 ccatcactca acttatccat aaaatcagca gctatcttat ctccctcctg agtaatatca
    20461 attagatatt ttcttctatc aactaaatct ctttttcttg ttacatactt cttatcagtt
    20521 aaatcatcaa ccatactagt taaactgcct ttttctatat ttaatttaga gcataaatca
    20581 gtcatactta ttactccata attttttatg aaaactaatg ctcttaattg tgttttattt
    20641 acattataat ctggtgcaaa tttttttagg tcttctaagt acaggctaga ataaatctta
    20701 ggaaatgttt ttactaaaaa acttatggta tcttttacta tatagttcat ataatcacct
    20761 cggttgtttt aaaatttatt aaattatcta aataacttta tttcagttta cattaaaagt
    20821 attgcaagta caatagtttt tgctgaatgt ttaaatttaa ttagtaatct tatcattttt
    20881 ttatttttat ttatattatt cttattaaat actatttata tagctatttt attaaattta
    20941 tggtatagta agtaaagaag aactcttcac agcggtgaag agaagacgct agcccattaa
    21001 aatggctatc ttatttataa ataagatagc cgtttttaat tatattttat ctgtacaatt
    21061 ctatactctt aggtctacat ctacttttcc taagtcttcc ttataatctt ttatagcagg
    21121 ttctacaact tcatttacaa attctacaac ttgttctgga gctctaccta taaagttttt
    21181 tgggtccatt atagatagta tttcttcctt actcatttta aatgaatcat cattcattat
    21241 aagttcaatt aagttattat ccttaccttc atgctttact ctataggcag ctttcataga
    21301 atattctctt attatttcat gtaactcctg tctatctcca cctcttttta cagcttccat
    21361 aagtattgtt tctgtagcca taaaaggaag ttcttcattt acacgtttat ttatcatatt
    21421 ttcatatact accaagccat cagttacatt tatccctatt tcaagtatag catctgctgc
    21481 cataaatgct tgaggtattg ccaatctctt attggctgag tcatctaatg ttctctctaa
    21541 ccattgagta gcttgtacca ttgcagggct tatagattca gagattatat acttagataa
    21601 agatgctatt ctctcacttc tcattggatt cctcttataa gccattgctg atgaacctat
    21661 ttgatttttt tcaaatggct cttctaattc ttttaaactt tgtaaaagtc ttatatcatt
    21721 agtcatctta tgcatacttt gagctatgcc tgatagtatg cttattactt gataatctaa
    21781 ctttctagta tatgtctgtc cacttactgc ataggaagaa tggtacccca tctttttaca
    21841 aattttctta tctaactctt ttactttttc gtggtctcct tcaaataagc ttaaaaaact
    21901 tgcttgagta ccagtagtac ctttaactcc tcttaacttc atattgtcta ttctatagtt
    21961 taaatcttcc aaatcaagaa ttaaatcttg agcccataaa caagctcttt ttcctactgt
    22021 tgtaagttga gcagcttgga agtgtgtaaa tccaagagct ggcatatctt tgtacttaag
    22081 tgcaaattct tttaaattgt ttattaaatt tacaagcttt actcttagaa gttttaaagc
    22141 ttcgttcatt tgtataacat ctgtattatc tccaacatat gcagatgttg ctcctaaatg
    22201 tattattcct ttggcttttt cacattgtaa accataagct tttacgtgag acattacatc
    22261 atgtcttact tctttttcta tttttctagc ttcatcaaaa tttatattat ctatattagc
    22321 tcttagctca tctaattgtt cttcagttat attgattcct aactcatgtt caccttctgc
    22381 aagtgcaacc cataaccttc tccatgtaga gaatttaaat tgtggagaaa atatataact
    22441 catgtccttg ctacaatatc tgtctgtaag tgggtttgag taagtagtat atttattatc
    22501 tatcattata ttagcacctt ccttaaatct tttgtcgtgt gattactgtg tacacattag
    22561 tacataaata attatatata attttacgaa ctataacaag actttttt
//
LOCUS       DAEORZ010000053        22231 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEORZ010000053 DAEORZ010000000
VERSION     DAEORZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.053"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            81..155
                     /locus_tag="KRH39_001886"
     tRNA            81..155
                     /locus_tag="KRH39_001886"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:113..115,aa:Thr,seq:tgt)
     gene            complement(305..982)
                     /gene="srtB"
                     /locus_tag="KRH39_001887"
     CDS             complement(305..982)
                     /gene="srtB"
                     /locus_tag="KRH39_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBG8511791.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            1114..1448
                     /gene="rnpB"
                     /locus_tag="KRH39_001888"
     ncRNA           1114..1448
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KRH39_001888"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1726..4269
                     /locus_tag="KRH39_001889"
     CDS             1726..4269
                     /locus_tag="KRH39_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBG8511792.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESVSQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENNEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGVKEEIDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERNVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            4392..6383
                     /locus_tag="KRH39_001890"
     CDS             4392..6383
                     /locus_tag="KRH39_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511793.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            6373..6693
                     /locus_tag="KRH39_001891"
     CDS             6373..6693
                     /locus_tag="KRH39_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511794.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            6891..7760
                     /gene="galU"
                     /locus_tag="KRH39_001892"
     CDS             6891..7760
                     /gene="galU"
                     /locus_tag="KRH39_001892"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG8511795.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            7784..8797
                     /gene="galE"
                     /locus_tag="KRH39_001893"
     CDS             7784..8797
                     /gene="galE"
                     /locus_tag="KRH39_001893"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBG8511796.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            8909..10843
                     /locus_tag="KRH39_001894"
     CDS             8909..10843
                     /locus_tag="KRH39_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBG8511797.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            10998..11861
                     /locus_tag="KRH39_001895"
     CDS             10998..11861
                     /locus_tag="KRH39_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBG8511798.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(12069..12950)
                     /locus_tag="KRH39_001896"
     CDS             complement(12069..12950)
                     /locus_tag="KRH39_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8511799.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            13363..14211
                     /locus_tag="KRH39_001897"
     CDS             13363..14211
                     /locus_tag="KRH39_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBG8511800.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMLAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            14290..16212
                     /locus_tag="KRH39_001898"
     CDS             14290..16212
                     /locus_tag="KRH39_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG8511801.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            16294..17157
                     /gene="aroE"
                     /locus_tag="KRH39_001899"
     CDS             16294..17157
                     /gene="aroE"
                     /locus_tag="KRH39_001899"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG8511802.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TIDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKNVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            17357..18574
                     /locus_tag="KRH39_001900"
     CDS             17357..18574
                     /locus_tag="KRH39_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8511803.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            18810..20675
                     /locus_tag="KRH39_001901"
     CDS             18810..20675
                     /locus_tag="KRH39_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBG8511804.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(20853..22217)
                     /locus_tag="KRH39_001902"
     CDS             complement(20853..22217)
                     /locus_tag="KRH39_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8511805.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
BASE COUNT         8839 a         2422 c         3897 g         7073 t
ORIGIN      
        1 cattcattaa gatatatgtg tagtttttag agtgttaact tttaaaaatt agttttacta
       61 atttacaata aaataggtgt gctggtgtgg ctcaacggta gagcagctga cttgtaatca
      121 gcaggttgta ggttcgattc ctatcaccag ctccagtaaa gactatgttt ttagcatagt
      181 ctttttttat tgtttgatgt atttgttatt ttgtattaaa tacttttagt ggtctaattt
      241 ataaattata ggtataaaag atgacacagt ttaaaattat tgttttaaac tgtgtttgtg
      301 agttttaaat caatctacca tgaatcacca ttctggcatc atcaaattca tatgtacatg
      361 tagaaagtgt aactatttta tcattgctat ttaccttaat aggagattta tacaatgatt
      421 tagaagtaat atcatttatg taattttgat aatcagattc attgttaaaa tttgttttta
      481 gataatcata atcagattct acaatataag cagaaaaaac atcatataaa aattcttttc
      541 cattcaaggt tatttttatt ttattatttt ttttaaagaa gtcagcatct ttaaacttat
      601 ttaggttgtt gaacatagtt ttatttttca tattatgtcc atatattatg atatttttgt
      661 catcaataga tttatttcta taatccataa acagtgtacc agaaatagaa tcttttttat
      721 aaaaatcttt gtcaagatag taagaattat ctttagattg tacaactgga taatttatat
      781 ttgtattctc aacagaaagc caaaatttaa aatcactgtt aatctttgat aaatcctctt
      841 ttttatattc ttttttttga agttctgagg atattttagt atcatgattg tatttggtaa
      901 gttttgagta tatattaaat ccactgtaaa gtattactaa tactagtata atgttaatta
      961 ctattctata taattttttc aatatatcac ctcgagttaa atgtagttta tatatctata
     1021 ttacactatt tttgaataaa tttaaatttt tagtataata ttactttgac atgtaaaaga
     1081 atttgtgtta acataactaa gtaaaataaa tattaagtaa gccagataat cgcatgtaat
     1141 cttacatgag gaaagtcgga gctccataga gcagggtgct aggtaacgcc tagtgggagc
     1201 gatcctaagg atagtgcaac agaaataaac cgccactatt gtggtaagga tggaaaggcg
     1261 aggtaagagc tcaccagcag ctaggcgact agttggctat gtaaacccca cctggagcaa
     1321 gaccaagata ggaataaaga gtgctgctcg tactctccgg taggtaggtc gcttgagcct
     1381 attggtgaca ataggcctag aaagatgatt atctaataca gaactccgct tatagacttg
     1441 cttaattatt tcgatagtca aaaacatcag tttaaactga tgttttttta tttgtaaatt
     1501 tgtctaatct acttttaaaa ttttgaggaa tattgtaata taagttagta cagatattaa
     1561 aaatggtata atataagata gatataaaaa gaaaaagtaa aatgatgtaa ataatatgat
     1621 atgatataag atgtagcaaa tttagtaaat aattatttag atatcataat caaattagca
     1681 actttgcaca atgtaaatat tacaataaaa taattgattt aaggagtgaa ggttttgagt
     1741 gaaaataata taataagaaa cactgtttta aatgtcacag caagtttttt aaaacaagag
     1801 tctaaaataa atgaaaagct agatggtgtt ttggaaaaga aatttgaaaa agtagaattt
     1861 aatgaagcaa aatatgcaga gcttttaaaa tttaatatat tgttttataa gacattagca
     1921 agaaatacag aaccacttat tggcaaatgg atagtagata agtacatacc tgaaatagat
     1981 gaattagaaa aagatttaga attaactaca gcaaaatgta gaaagtatgt aaataaggct
     2041 atgaaagagg gattagactc tctaaaagcc aatgatttaa atagtttttt agcttatgat
     2101 aaaatggaac tatcagagag aagaagacgt ttagaaaagg attataaggt tttaaaccta
     2161 tataaagacc ttcttaatat atctcttaga aaaataagtt tagaaaagaa agaatgtgga
     2221 gatttatttc ttaaaaatca agcagatgcg aaaagagaat taaaaagaga aattatattc
     2281 tgtgtaaaca aaatattagc tagcaataaa gatgtggtac ctaataatgt ggaagagaat
     2341 aacgaacaag acaaagtaga aataaaagca actgaaattg aggatttgca attaggtaaa
     2401 gataattcag aaaatgacat gctactaaat gaaattaaaa attcaaaagt aaaacatgaa
     2461 tcaaaatcaa atgatataga atcgaagttt atagataagt taaatagctt aaataatgaa
     2521 agtgtatctc aaacaataca caatgaatat aagaagttat gtggattaga agaacttcat
     2581 tctgttgaag gatatggttt tggcaaagaa attattaaag attttgcttg tgcaacggtt
     2641 gtattagaat ttttaaagag aagaaataga gaccttatag aaggtgcaat gagactgact
     2701 ataattggag aatttggtcc tgaaaatttt aaggaatttg tggactatgt aattaaaaat
     2761 aaaacagaaa ttagtgaaga tacatggaat aaagcacaga atctaataaa agataactat
     2821 agtgaattag aaaataatga aattgcatca aaaagaacta gaagaaataa agatatcgat
     2881 attgaagaat acatttacat gataaaaaat gcagataaag atatttgctt tagaagtagt
     2941 atttcaatag aagatgatgc tgaagaaggg gttaaagaag aaatagatag taataatcaa
     3001 gatataggtg acgttgtaga agataaagat actacagata aagagtacga cagcaataaa
     3061 gaagatataa ttgaaccaga aaataaaaag agtaaaaaga aggctaaatt atttggattt
     3121 attaaaaaag acaatgaaga agttgaacaa gaagaagaga atttaaatga tatttctcca
     3181 gatataattt tagataaacc agttgaaaat catcaagtga aatcagaaga aatagagcaa
     3241 aatgaattaa aagaaataaa acaagaagaa ccaagtcaac atatagaaga agaacgtaat
     3301 gtaaaaatag aaaaaccaat aaataataac ttagatgaaa aagtatcttc aaataatgaa
     3361 tcgaagcttg aaaaagaaag taaaaactta gaagataaaa aagcaaaaga aataaaagaa
     3421 gaaaaattag aaaatgaaaa atcagtagta atacctatta aaaagaaaga aaatagtaat
     3481 aaaaaatcta aaaattcttc taaggacaaa tatagagaaa acaagaaaga aatgaaaaat
     3541 tatgtatcag ataaagaaga ttctttagat gatgaagagg ttgtttctaa gaagagtaga
     3601 ttaaaagaaa ctattatagc agtagttata gtggttattg ttggtgtagg atattttata
     3661 acagtaggca acaataagaa aaatgataaa gagaatattc caaagtcatc aactcaacaa
     3721 caagctaaca ataaattaac tgaagaagag aaaaaagcac aagctgaaaa agagaaaaaa
     3781 gaagcagaag agaaggcaaa agctgaaaaa gaggctcaag aaaaagcaca agctgaaaag
     3841 aaagcaaaag aaatggaagc atataaagat ggtaaaggtg tatattacac tgtttatgca
     3901 ggttcactta aagttgaaaa aacagcaaaa gaaacagcaa aagaatatga ggctaaaggt
     3961 atttcttcaa caataattca agaaaatgga tactataaaa taaaaatagg tgattattct
     4021 caatatggag aagcacaaga aaaatgtaat gaactagcta agaagtctat tgatacatat
     4081 atagctatgt atgataagta ctatgattat aagttagaag aattaaaaga atcagctcct
     4141 tctttgagtg ctgaagaact taaacaaaaa tatgaagatt taagaagtga gttaaagaac
     4201 aaatctggat atcgtgaata tgttaaacat ttagataaac tttatgaaga aatagttgaa
     4261 ggtgcttaga gcattataaa aaaggagaaa ctatgtctca gaataaaaag tattaaaaaa
     4321 aatattattt gttttgagac attttattca cctttataaa atacttataa ttctgtttag
     4381 gtggtgaagg tatgttgaaa ttaggagaaa aaatcatata tgagctttca gatgggtttg
     4441 agcttagtgg cataagtaga tactcaaaag aatataaaaa gcttcatata acaaatctag
     4501 ggaatataat aatcagcaaa tcagatgaag ttaatgagga tacaggtgta aaatatatat
     4561 atagtttttc agacaataaa gaaaaattag aggcagtttt agaagattct caaataattg
     4621 tctatggaaa taacttgcct ttttacataa aaagtgaaaa ttcaagtaaa aatgttacag
     4681 caaatttgac tatgaaaata actttggatg aatatgattt aatctgcaaa agtcaaagag
     4741 aatttatatt ctatctaaag gataatatga ctatattaag tttagatgac aataagtttt
     4801 atatgggtgg aattaataag gataatgaga agtttatatt tatcagtggg aaaaatagat
     4861 ttgaaataaa ttttgatgat atagaaagat acattttaga agataaaaga gtgtcattga
     4921 agggatactt tcatatggaa agagagggga ttatagtaag gtctgtatct atatttaata
     4981 acaatataga tagtgtagtc ccatctgact taaatgagag agttaaagat aaccaaaaga
     5041 taggaaatct tcctaaagat tgtgaaatag tattctgcaa gatatcagga aatatagatg
     5101 gatttgatta taaaaacaca aacatgcttt tagtaaaata tcaagaccaa ttaattttta
     5161 taaataagaa atctaagaag actattgtaa agtcagcgaa agataattgt tctaagttaa
     5221 atttaggaga agatataata ttatatgata ataaaaatgt atttaaccta catataaatg
     5281 ataaaaatag agaaattatg caaatagatg acttaaaaga tatagaaaat gaaatagtgg
     5341 gatatacatt aaaacatgct ccatttttta ttcaagaaga tttcgatagc ttgactattc
     5401 taaagtcttt tcaaaaagaa atcatatcta taaaaaatag tgatataaaa gacattgtaa
     5461 ttaataaaga acttgaaaac gaaaattcta attttgtaga aacggaaata aaatttaata
     5521 atcaaaaagt acttttaaac ttaagtaaaa atatggttca aaaacttatg caagatgtat
     5581 ttatatatgc aaagcaacct ctacttaaag aaaattctat tgaagtgata tataagaatt
     5641 ggtctaaagc tatgaatgat atgataatat ttaatttctt tggaaatatt tactatatga
     5701 aatctgaatt tgataagatt ttagaaaaag agttaaatga tgaaataaga atagaagtta
     5761 taaattcact ttataagcaa atacaagaac aaagaaacaa tttagattta ttgtcagcat
     5821 atatgccaag aatattagaa aatcaagaaa tagatttgtt tgaaaagtat aatactaaac
     5881 ttgatgtaca agtgtttaag cagattaaaa atctattgtc tgacttatca tataatataa
     5941 gcagttattt aaatgaagtt gaaaaaagtt tagacaatat catctttgta atttcaggag
     6001 aagataagaa gaaatacaat tatagaatgt taaaagaaag tgaaagtgca agtcttgatg
     6061 tatttttaaa acaagccata tcaagattaa accatttagt agaaaatatg tatccttatt
     6121 atgtagatga aactagtagg gaaatgttta agttatttga gttattgtgg aaaaactata
     6181 gaaatattga tgatgatagt ataaaagaaa tattattcga gagaataaca aatacatatg
     6241 tatttaaaca attgacatta aataatagta caaaagaaag aagaaaagac ataattgaaa
     6301 aaatatataa ctctgttgat tatggaacca ataaactaga tgaaaatatg tttttcacag
     6361 gaggaataaa gtatgtcaag taaatacaat gtttggactt ttaactatga gttcttagga
     6421 ttggactcag gggatgaaaa aaataatgaa gtttattacg agattgattt agatgaaatt
     6481 ttaaatgaaa actatataga tgaagatata gatagtggtt tagattctga tggaaatagt
     6541 attttaggag aattaatatc agaaatggat atagatgagg atgtcagtgt tgatatgaca
     6601 tatcaagaac tagaagttga cttagaagat ttagattctt atctagatga agatatagat
     6661 agtgatataa aaggcatctt agatgaaatt tagtacctgt ataagttttg gataaaatat
     6721 aaacaataca atattaactt ataaaactaa aggtagatga aatttcatct accttatatt
     6781 atcttatagc attaaatatt gggataatat agagtttgaa gaaaaagtat aaatatggta
     6841 tattaatttg gtacaataga aaatttacga agaagggtga aaaaatgcag atgacagtta
     6901 aaaaagcaat aataccagca gcagggctag gaactagatt tttaccagca acaaaatcac
     6961 agcccaaaga aatgcttcct atagtagata aaccaacttt acagtatata atagaagaag
     7021 ctatagaatc gggaattgaa gaaattctta taataacagg tagaaataaa aaatctatag
     7081 aagatcattt tgataaatca gttgaattag agttagaact agagcaaaaa ggtaagacag
     7141 agatgttaga aatggtaaga gacatatcaa atatggtaaa tattcattat ataagacaaa
     7201 aagagccaaa aggtcttgga catgcaattt actgtgcaaa aagttttata ggtgatgaac
     7261 cttttgccgt attattaggt gatgatatag tagattcaga tgttccgtgc ttaaaacaat
     7321 taatagatac ttataatgag tataaaacaa ctgtattagg tgttcaaaag atagctaaag
     7381 aagatactaa taaatatggt atattggatg taaaacatat cgaagataga gtatataaag
     7441 ttaaagatat ggttgaaaaa cctgcaattg aagaagctcc atctgatata gctatacttg
     7501 gaagatatat aataactcct gcaatattta gcatattaga gaaacagaca cctggtaaag
     7561 gtggagagat acaactgaca gatgcacttc aaactcttgg aaaacaagag gctatttatg
     7621 cttataactt tgaaggtaga agatatgatg tgggagataa gattggtttt ttagaggcaa
     7681 caatagactt tgctctaaag agagaaaatt taaaagatga tttaatggac tatatgagaa
     7741 aaaaagtaag tgaaaaataa atattagaaa agaggtaatt acaatggcag ttttagtagc
     7801 tggaggagca ggatatatag gaagccatac agcaatagaa cttttagaat caggttatga
     7861 agttgttata gttgataact taagtaatag caactcaata gtggttgata gaatcaagga
     7921 attatctaaa aaaccagtaa aattttataa tattgatatc agaaataaag atgaaatgca
     7981 tgttgtattt aaagaaaata atattgaatc aattatacac tttgcagctt taaaagcagt
     8041 tggtgaatca gtagaaaaac caattgaata ttacagcaat aatctaataa gtaccttaaa
     8101 tttatttgaa ttaatgagag aatatggtgt taaaaagttt gtatttagtt cttcagctac
     8161 tgtatatgga gacccacata cttgtcctat attagaggat tttccacttt ctgtaaccaa
     8221 tccatatgga agaactaaac ttatgataga gcagatgtta gtagatatat caaaagcaga
     8281 taagtcattg gatatagctc ttttaagata ctttaatcca gtcggtgctc ataaaagtgg
     8341 tagaataggt gaagaaccaa atggagttcc gagcaatcta atgccttata taacaaaaat
     8401 agcagtaggt aaattgaagg aattgagtgt ttatggtaat gactatccaa cacatgatgg
     8461 aactggagtt agagactata tacatgtatt agatttagct gctggtcatg taaaagcatt
     8521 acaaaaatta gaagaaaatc caggacttgt tgtttataat ttaggaactg gaaaggggta
     8581 tagtgttctt gatttagtaa aagcattttc taaagcaagt ggaaaagaaa taccatataa
     8641 gattgtagga agaagggctg gagatgtagc catgtgttat gcagattctt caaaggcaga
     8701 aaaagagctt ggatggaaag ctaagtatga acttgaagag atgtgtgaag attcatggag
     8761 atggcaaagt atgaatccta atggatatga agagtagaat acatacttat atgtataaat
     8821 gtaacaaatt tgtaaatata aggtattggt aattttatgt tataattaga cacatgtagt
     8881 aatgtttcta aaagaggggg aagttatagt gaaaaaaagt ctaaaaaagt atttagtact
     8941 agcactgact ttagttttag tatttgcatg tggcgtacca gaatcaagcg cagccagtaa
     9001 acatgtgatt atagtaaatt caagaaaaaa tactctagga tattttgtaa ataataaact
     9061 tgtaaaagaa tttagagttg caacaggtaa aaaggggtca gaaacaccaa cagggaaaac
     9121 aaaagtagtt aacaagataa aaaatagacc atattataaa ggtaatatac ctggaggaag
     9181 tccaagaaat ccattaggag atagatggat gggattagca ttaaaaggaa cttatggaga
     9241 tacatatgga atacatggaa ataataatga gagttctata ggaaagcata tttctggtgg
     9301 atgtataaga atgcacaata aagatgttag atggttattt gaccaagttc ctgtaggttc
     9361 agatgtaatt atagattata gtaacgacag ctatgtaaag atagcggcaa aatataagat
     9421 aaatctaaat caaacaggat ggaaaacaga aaatggtaag aagtattatg taaaatctga
     9481 tggaacatac caaaagaata gttggctaaa agtaaatgga aaaatgtatt actttgatgc
     9541 ttcaggagta atgcaaacag gatggaaaac aattaataac aaaaaatatt atttaggaac
     9601 tgatggagca agagtatctg gatggaaagt aatagacgga aaaacttatt actttaactc
     9661 agatggagta atgcaaacag gatggcaaga aaagaatgga aagaaatact atctaggatc
     9721 tgatggttta gcagtaacag gatggcaaga aatagatggt aataagtatt actttgacaa
     9781 aacaggaata atgcagacag gatggcaaca aatagatggt aagtcttact atttagacaa
     9841 agatggaaag atgctaactg gttctcaaaa aatagatggg aaagattata cttttaatga
     9901 agatggaact ataaatccta catgggatac aattataggt gcaaatagat ttgatacagc
     9961 taaaaagata agttcagttg gaaactggaa tgctgatagt tcagatactg taatattagt
    10021 aaatggtaat gcaatagctg atggtataac agcaactcct ttagcaagta gctatgactc
    10081 aacaatacta cttactaata cagctaattt accaactgaa actgttgaag aaatgaagtt
    10141 attagcacct aaaacagtaa ttttgatagg tggagaaaat gcaataagtt ctaaattaga
    10201 gcaagaaata aaaactactt tcaacacaga aacaaagaga atagcaggtc aagatagata
    10261 tcaaactgct acaagaatag ctgaagaact tggaaatcga gaagaaataa aaactgcata
    10321 tatggtttct ggaaatgggg aagcagatgc tttatctgta gcttctaaag ctggggaaga
    10381 aaaacaacct ataatattag tcaataaaga tggaattaca gaagaatctt ataaatggct
    10441 aacagaaaga aaattagaaa atgcatactt tataggtgga ccaagtgcaa ttaacgattc
    10501 agtaatagca aaaatgaatg atataactac tgaagatatt tcaggaaata gaatatatgg
    10561 agatagtaga gtagatacta atgccaaagt aatagaaaaa ttctatggag atactgattt
    10621 acaggctgta ttagttagta aatcagatgc tttagtagat gctttatcag ctggaccatt
    10681 agctgtaaaa ttacattcac caatagtttt aatggataat tcaggattat catcagagca
    10741 acaaagagta tttgcaaata aaaaagttga aactccttat caaataggag gcggagtatc
    10801 ttatatagta atggataaat taatggatat tcttgcaaaa tagtaaaaat atatattgaa
    10861 aaataattat aattgagatg tgaaattcaa tcaataaaag cttgattgtt ttacatctca
    10921 attttttagt ataaaattaa gtttaagtta tataaaaatc ttggaatatt tttaaaactc
    10981 atataaaatg aatatagatg ctatcatcat atgttaagag ggtgagtaag attataaaga
    11041 aaaaatatat aatgtttatt gatgaaagtg gaagagctga tataaatcat gaagacccat
    11101 ttacattgac aggagtaatt tttgaatata aatatgcagt aagtcaaggc gattatgtat
    11161 gttctttgag gaaagaaatg gatgcattta aagagtattg ttttggaacc agtaacatag
    11221 gaatacattt gacagatata tcaagaaaat caagagcatt tgagaagttt gatgattcac
    11281 agattaagtt attctatgat gagttgcctt tatttctatc tagattagag tgtactataa
    11341 tatcagtaac agtagataaa gctagattaa agaaatatta tgcaccttca aaggaaccgt
    11401 atgttgtggc ttttatacat gttcttcaga atttttattc atttgtaaat aatgaaactg
    11461 tagaatctgc acgaatagtt atagagtcaa gggatgatgt ctcaaactta aaagtacaaa
    11521 aggcgttttt tgatgtattt aataatggaa caatatactt agatatagat gaagaattaa
    11581 gagataaggt taagggtttt ataattgcca aaaaagaaga tgctgactat agagaaggtt
    11641 tagaaatagc agatatttta tgtaatcctc ttagcagagc aagaagaggg ttaatagaag
    11701 ctaatccaaa gtgtatgcaa tatggagaaa aaaacaaaat attcaaatct gtcagaaaca
    11761 aaatatatac tccaacgagt gtgaatgata taagaaattg gggatttaaa gaagttccta
    11821 ttgtagagcc tgttggacca tggcttgaaa aaatgcgtta gatacaattg acaaatttta
    11881 aaaaatctga tataatgtaa gtaacctagt tattcaggaa ctgaatccag ttgctgttgt
    11941 ataacgataa ttactgagac tcggatgccg actgtcagtt aggaatctat acttttaaaa
    12001 gtatagattt ttttatttta taaaaagaat aaaagaggat atttaaaaat aaccccttta
    12061 ttaataaatt ataattgagc gtttttaatg acaaagtttt taaagttttt aattgcagga
    12121 ggcaaatatc tatcttttat aaatgctaag taaaagtttc tttcccaact tgggtgaata
    12181 atctgaatta cttttacttt tgtaaagcta agcatattca tatttgggac tacagcaatt
    12241 ccgaaatttt ttgcaactaa tccagcaatt acttggtctt cctctacttc atagactata
    12301 ttaggctgtt gatttatttt tttaaacata tcatcaatta tatatctaag accacttctc
    12361 tgattaaata ctatttgagg ataaggtatg gtttctttta aatctatagt atcctttgca
    12421 gctagtggat gactatatgg tacaattagt actaaatctt gtttggcaac aggtataaat
    12481 tcaattccct tttctttttc aagtttcgaa caaaatgcaa tatcatattt tctttcttta
    12541 agaccttgaa taatgtcagt agttacacca gtatgaaatt taaaatctat tgatttagca
    12601 ggattagatt ttaaaaattt gtgcactatg tctgggataa aatcagtacc aagtgttcta
    12661 agaaaagcta agtctatttg tccttcacca gtaccagtta tttttaatga ttttacactt
    12721 gaatctaaaa tttctaatga tttttccaca tcttttaaaa atatttttcc atacttagta
    12781 aggactatat ttctaccttc tttttcaaat agagccaccc caagttcatt ttctagtaaa
    12841 gaaatagcat gacttaagct tggttgtgtt atagataaat tttctgccgc ttttgtatag
    12901 tgttctaaat gtgccaatgt aacaaaatag cgtaagtgat ataaattcaa tttttgttca
    12961 tcctttcata gttattttta tttatatatt tgtagaagtt aacctttaag atatttttac
    13021 atttttatta tatatgatta tagagtaaga ataaaagctt ttaaaaaaat ttatcgtttt
    13081 ttgtctgaat tataagataa atatatttat acgacctaat agaaaaatac aagaattatc
    13141 ttataaataa aatctataaa caaaatctat gaattaatag gaaattatca atttgttatt
    13201 agtaaagtct cgtgatacaa taaatttata aaatctagat aaacagaaat caaaaaatac
    13261 ttttattttt aaggtaaaat aatactacta gtaggataaa taaaaatata aaatcaaaaa
    13321 tactattaat tataaagaaa ataaaggggg caaatttaag ctatgaataa tcaaaaaaat
    13381 cttaatattc caattacaat tagttcttat acattgggaa cagaagtttc attccgtgat
    13441 agagttcgta ttgcaaaaga agctgggttt gatggtattg gattgcgtgc tgaaaattat
    13501 attgatgcaa aaaatgcagg tgttacagat gaagaaatgc ttgcaattct taatgaatat
    13561 gatatgaaag ttacagaagt agaatatata actcaatggg gaagagaaga agatagaacg
    13621 gaagctcaaa gagaaaaaga gcaaaatgtt tatcatatgg ctcacttatt taatgttaaa
    13681 catataaatt gtggattatt agagatgata ccagaagaac aaattataac tgcattggga
    13741 gaattatgtg acagagcagg tgaacttatt ataggtcttg agtttatgcc atatagtggt
    13801 gtaccagatt tagctactgc atggcgtgtt gtgaaaggat gtaatcgtga aaatgcaatg
    13861 cttatattag atacttggca ctgggctaga gcaaagcaga cagcagagga tcttgcacct
    13921 gttccagcag ataagatagt atcaattcaa gtgtgtgatg ttttagaaac tccttatgaa
    13981 aaacttaggg atgaatcact acatgaccgt ttggctccag gtgaaggata tggaaacaca
    14041 gaggaatttg tgaaaataat aaaagaacac aatatatcac caagagtcat aggggcagaa
    14101 gtaatatctg attctctaat atctaaaggg ttatccactg ctgctgaaac tgtatttaat
    14161 gcaactaaaa aagttttgga taaagcatgg tctgaagtat ctccaaaata aacttatata
    14221 atataaaaat atacagagta ttataatata aaacttttaa atataaaact tgaaaggaag
    14281 gaaaacctta tgaagattag ttctatgttt acacctataa gaattggttc aatgacagtt
    14341 ccaaatcgtt ttgtagttcc ccctatggga aacaattttg caaacacaga tggtacttta
    14401 agtgaaactt ctaaggctta ttatcttgag cgtgctttag gtggttttgg attaataact
    14461 atagaatcta gtgttgtaga caaaaaagca aaaggaggtc ctcgtaagcc ttgtttatat
    14521 gatgatagca caatagatag ttttaaaaat gtaatagatg cttgtcatga tgcaggttcg
    14581 aaggtatcta tacaattaca acatgctggt tctgaaggaa atgaaaaagt agcaggacat
    14641 ccattaaaag ctgcttctgc aatacctgca tcaaatggtc gtaatacacc actagcaata
    14701 acaacagaag aaatatatga acttatagaa tcttatggag atgctgcatt acgtgcacaa
    14761 aaagctggtg cagatgctgt tgaagttcac tgcgctcatg gatatctggt cagtagtttt
    14821 atatcacaaa gaacaaataa gcgtgtagat gagtttggtg gatgttttga aaatagaatg
    14881 agattacctc gtcttatcat tgaaaatata cgtaaaaaag taggaaattc tctagcaata
    14941 ttatgtcgta taaacagcac tgatgatgtt ccaggaggaa ttgatgtaca cgatagttca
    15001 gtaatagcag catatctaga agattgtgga atagatggac ttcatgtttc acgtagtata
    15061 catatacatg atgaatatat gtgggctcct acgactttac atgctggatt cagtgcagaa
    15121 ttagttactg aaataaaaaa agctgttagt ataccagtaa taacagttgg acgttataca
    15181 gagccacagt ttgctgagtt aatggtcaga caaggaagat gtgatttagt agcattcggt
    15241 agacaaagct tggctgaccc agaaatgcct aataaagcta aaaatggaaa actagacgaa
    15301 atgataccat gtattgcatg cttacaagga tgtgtaccaa atatgttcca aggcaaacct
    15361 atagcctgtc ttgcaaatcc tatattagga catgaagcag agcttaagcc agctgaaatt
    15421 agcaaagaag ttcttgttgt tggtggaggt gttggaggta tgcttgcagc atgggtatgt
    15481 gctaaaagag gacataatgt aactttagtt gaaaaatctg aagttcttgg tgggcaaatg
    15541 cgtttagctg catatcctcc aggaaaagga gatattacta acttagttag aagttatatt
    15601 tcaaaatgta atcaatatgg tgtaaaaata tgtactaata cagaagctac agtagaactt
    15661 ataaaggaga aatcacctga tgtagttatt atagctactg gagcaactcc acttgtactt
    15721 cctataccag gaatcaatga ttcaggatta attcatgctg tagaccttct tgatggtaaa
    15781 aaatcttgtg ggaaaaaagt acttgttgtt ggaggtggaa tggttggatg tgaagtggca
    15841 gccttccttg gagaacaaga acatgaggtt actgttattg agcttagaga agaagttggt
    15901 gcagatgtaa tttcagaaca ccgtaaattc cttatgaatg attttgaaaa ttataatata
    15961 cataccatta ctggtgcaaa agtttctaaa ttttatgatg atggtgtcga ctatttactt
    16021 acagatggca cagagcatga tttaaaaggt tttgattcag tagtacttgc tatgggttct
    16081 cgtaattacg acccattaag tgaagttata aaagaagttg taaaagaaac ttatattgtt
    16141 ggtgatgcag taaaagctcg tagggcatta gatgcaacaa aagaagcttt tgaagtagca
    16201 ttaaatatat aaataataaa taaatttttc tttgtaaata actgaatagg ttatttgcaa
    16261 agaaaatgat aatattggga ggagattata gttatggaaa tttcaggtag aacaggatta
    16321 tttgcattaa taggtacacc ggttggtcac tctaaatcac cagtgatgta taattatagt
    16381 tttaagaaat tagatttaga ttatagatat ttagcctttg atatcacaat agataaggta
    16441 aaagaggcac ttttagcaat aaaaactttt aatataaaag gagccaatgt tactatgcca
    16501 tgtaaatctg ctgttacaga atatatggat gaactttctc cagcagctcg tataattgga
    16561 gcatgtaata caattgtaaa tgacaatgga aagttagttg gtcatataac agatggagta
    16621 ggctatgttc gtaatttaaa agaaaatggg gtagaagtta aaggtaaaaa gattacaatt
    16681 atgggtgctg gtggtgcagc aactgctata caagtacaat gtgctttaga tggagcaaga
    16741 gaaatatcta tctttaatcc taaggatgat ttttataaaa gagcagagca aacagttgag
    16801 aatataaaaa aaaatgtacc agaatgtgtt gttaatctat atgatttaga agatacaaac
    16861 aaactttatg aggagataga atcaagtgat atattgacaa atgcaactct tattggtatg
    16921 aagccttatg acaatgaaac aaatataaaa gatactagtg ttttaagaaa agatttagta
    16981 gtaacagatg tcgtttataa tcctaagaaa acaaaaatga ttgaagatgc tgaagctaat
    17041 ggatgtaagg ctattggtgg attaggaatg ttattatacc aaggagcaga agcttttaat
    17101 ctatatactg gattagaaat gcctgtagaa gaagttaatg aattatgctt taaatagata
    17161 agagtaatat cttaaacaat gaaaaaacat acgtatttca atatttttaa taataaatta
    17221 caaattgtca tagtgggttt tgcctcatag tccagttaaa tttgaagctt taactgacta
    17281 tactttaaat ccattatggg gatgagttta ttgtacacaa aaatgttaat aaattaacaa
    17341 gaatgggaga aaaattatga acaataaaaa atattatcct actgccatag cgttgtattt
    17401 ttcatatttt ttacttggta ttggaatttc aattttagga caatacaaac cagaattttc
    17461 atcaatgtgg ggggctaaaa cattatcaga tggaacattg gatgtaagta ttgttttagc
    17521 tgttattgct gcactaggat tagggagact tatatcttat ccatttgcag gacctatttc
    17581 agataagtat ggaagaaaag taagtggtct tataggaaac tttctacatg caattttctt
    17641 tgttggaatt gtattttcac caaattttta tatagcatat gtatttgcaa taattggagg
    17701 ggcagcaaat tcattcttag atacctgtgt aactccttct tgtatggaga tatttgctag
    17761 tttaggaaca atagcaaata tgtttactaa atttacaata gctttagcac aatttttgct
    17821 tccttttata attggatttg ttgcagcaaa ttcaatatca tttaaagtta tttttatagt
    17881 aacagcgatt cttattgtag tagatgcctt tttgatagct gtgttaccat ttccaccagc
    17941 taataatgtt attgataaca aaggcaaaac agttaaatca gagaagatga aatttacacc
    18001 aacaagtata gctcttgttt gtattggatt tacatgtaca tctacatttg tattatggtt
    18061 aaattgtaat caggaattgg gtaaattgta tgggatggct gaccctacaa aaattcaatc
    18121 cttttactca atgggagtaa tatgtgcagt attaataact tctttactta ttaaaaaata
    18181 tattaaacca ataagaatat tagttatata tcctataata gctttactta tgctattagt
    18241 tgtatatttt gttcaaactc caactatttg tatgttaggg ggatttgtaa taggttattc
    18301 agcagcagga ggtgttcttc agttaacaac gtcaactgca aatgaaatgt ttccaactaa
    18361 taaaggaaaa ataacatcta tagttatgat tgcatctagt atagcaaact atgttatttt
    18421 aaatatagca ggtattatta caaaatcagg gggagttaat ggaccaaaat atgttgtatt
    18481 gtttaatgtg gcaataacat ttgttggaat tttacttgca ttgtttgtaa acatgagata
    18541 tgaaaaagaa aaagtgtatg attatgatgt ctaatattag tataatatat gcatagagaa
    18601 gtctaaatct aggatagatt tttattaaaa gtaattaaaa ttaataagga tattatttac
    18661 agttataaga tagggtattt acataattaa taataaggat attactagaa tttcaatttc
    18721 tagtaatatt tttttgcgta taaatgtaaa cttgacgaaa taagtagtat aatcaatata
    18781 aattacatca tgtaaaggag gataatttgg tggagaatgt ttttaagcga ttgcaggaat
    18841 ttaatggtta tgatggatac aaagaatcat ttgaaatgaa ttacctatgt atatatgaaa
    18901 gtataccgtt aagagaacaa gtagagcttg caaataatct agtagatgaa attttaaata
    18961 tgtataaaag tgaatctaat gaaatttatt tattggagga tagtaattct aagtctttaa
    19021 tttgttattt cgaaattttt atgaaaaaga taaatacttt ggtaaaagaa atgattattg
    19081 atgagaaatg gttatataaa ttaactaaag aattaatata taagagtaaa aaagttgagt
    19141 atgtaaaact agggttagtt ttaagtgaga aatatctaaa tgtagaaaac ttaagagaag
    19201 ttgtagatac attttctaaa tctggagaat acgtatttta tctgagtaat actattaaaa
    19261 aacttgaatt ttacaataca tatttattta atttatctaa aaaggctaca ggttcaataa
    19321 aagtatttgc tattgttaat atggaaaatt tagattctaa aataaattcc tatctaattg
    19381 aagatggtta taaggataca aagtacgaac gattattgat gaactatatt atttcaattg
    19441 tagatttgaa tgaatatctt gaaaaacgag atttagataa agaaaagata aataatttag
    19501 cacgtcttat atgcaattac ttgctaagtg tagaatttaa atatataggt aataaattag
    19561 aattagtgaa tcgattttta cctactgtag taaattatgg gactaacttt gaaagtttat
    19621 attcaatttt cctaatagct ataaacgtct taaaagatga aaatatagag tgcaataaga
    19681 ttgaatttga aaaagaaata aatgatatct tgctcagtga aaaatggaaa aatatatatt
    19741 ttgaagcatt aagagatgct agtggaaaaa cagaagatat tataaaaatg tcagaaattt
    19801 atgatgtaaa cttatctttt gatgacttgt tgccatatct aaatagagat attagagatt
    19861 ttgaggtata ttggcatata tctaaaaagg gaacgacaag ttctagatta aaattattaa
    19921 atttttttga agagacgttt aaaatagatg atttaattgg aaaaatgaaa gatatagaaa
    19981 aagataaatt gactcaagaa tattatgatg atatgttatt ttttattgtg cttaaaggaa
    20041 gtaaatcact atatcctgaa ggaaagaata tatctttaaa aggaatattt ggaaatataa
    20101 atgaagtaag aaaagaatcc ataaacatct taaaaagata cagagagaaa ctatctcttg
    20161 aagaattaaa aattgttaaa gaagcgtatg aaaaagaaaa aaatgttata ttgaaggatg
    20221 aattaagaag agtattatat gaaagtaata atttaaaaaa agaatttgta aatattgaaa
    20281 aaataaaagt agatgaacat ggtaaggata tctatttgac ttcaatagct gttgctggtt
    20341 caaggtttag aaatagagaa tatcttgaaa aagaattgga gaaaagtaag atatactatt
    20401 taactagaga gaaagataat ctatatgatg aaaaagctat taaaatagtt ggtgaaactg
    20461 gatatgtaat aggctatgtc ccaagaaaag aaaactatat tttgagcaat ttattagatg
    20521 gaggaaaact gctgtattgt agagtaactg aatataattt atatgaagat tgtatctacg
    20581 caaatgtgta cttgagttat aaagatgtaa ttgaaacagt tgaaaactct ttaaagatgg
    20641 ttttagataa gagtagaata aaacttataa actaagacgt ttatatattg cttagatgat
    20701 gtaatcttaa ctgattggtt attttttcta gaattatggt gtattttatg agcaatgcta
    20761 atgaatataa aataaaaaaa caattgtttt gtatatatga tgtatataaa tatatttata
    20821 tattcatatt tacaaaacaa ttgaaattta tgttatttta taattttaaa tttagatgga
    20881 attttaactt ctgctttatc ttttatttta gaagcttgtt ttttaacttt atttaataat
    20941 tcagattcat ctacaactaa tacttttctt tccttcatta accatttacc atcacaaata
    21001 gtagagtcta cattttcact agtcatagaa taaactatat ttccaattgg attatgcatt
    21061 ggtaaacaat gaattgtatc tggatttaga ataatcatat cagctttttt tcctacttca
    21121 agactaccaa tttctttttc tagtaaagca cattttgctc catttatagt agccatttct
    21181 aagacttctt cagctggtac gacagttggg tttaaagttc ttcctttatg tattagtgat
    21241 gtaagataca tgtccctcat aaggtccatt ctattatttg aaggagctcc atcagtacca
    21301 attgaaacag gtattccttt ttctaacatt tcaggaattg atgcaaatcc tagaaccact
    21361 ttcatagctg aacctgggtc atgagaaact tttacatcat gtaatctaaa taaatcaatt
    21421 tccttatgag taagccaaac tgtatgagca gctaaaagat ttggtcctaa tacacctagt
    21481 ttatcaaggt attcaactgt tccaactcca ttgttttgct ttacaaatga aatttcatca
    21541 gctatttcag caatatgcat atgtatacct gtattgtatt tatcagctag ttctttagtt
    21601 ctaacaagta attcttcagt tgtattaaaa atagttctta atgcaaacca tattttaatt
    21661 ctctcattag atgtattatt aaacttatgg aataagtcta attgagtatt gatttcctca
    21721 tcagtagttt tttgccatgc ctcaggaaga ccatctcctg tatccataac tgatttagct
    21781 aaacaggctc taagtccagt tttatcaaca gcctcaacca tagcatctac atattgacca
    21841 ccagattcta aaaatgtagt gacaccagat tttatcaatt caacacaaca tgcagtagat
    21901 gaaatcaagg aatcttcata attgaagcta ctttcgtatg gccaaattct ctctctaagc
    21961 caagttaaaa gtacaacatc atctgcaaga ccacgtccta attgttgaga taagtgaaca
    22021 tgcgtattga ctagccctgg cattagtatt tttcctttag catcatatat ctctgcatct
    22081 ttatctacaa tattaatatc aaacttacca acctctttta taatattatt ttcaataagt
    22141 atgcttccat tttcatatac ctctctatct gcattcatag taattagata tgcatttttt
    22201 ataagtaatt gtgacataaa caccactcct t
//
LOCUS       DAEORZ010000054        22123 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEORZ010000054 DAEORZ010000000
VERSION     DAEORZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22123
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.054"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            445..1515
                     /locus_tag="KRH39_001903"
     CDS             445..1515
                     /locus_tag="KRH39_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8511806.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1499..2368
                     /locus_tag="KRH39_001904"
     CDS             1499..2368
                     /locus_tag="KRH39_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG8511807.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2412..3272
                     /locus_tag="KRH39_001905"
     CDS             2412..3272
                     /locus_tag="KRH39_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG8511808.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3303..4607
                     /locus_tag="KRH39_001906"
     CDS             3303..4607
                     /locus_tag="KRH39_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8511809.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4670..5932
                     /locus_tag="KRH39_001907"
     CDS             4670..5932
                     /locus_tag="KRH39_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBG8511810.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6119..8089
                     /gene="uvrB"
                     /locus_tag="KRH39_001908"
     CDS             6119..8089
                     /gene="uvrB"
                     /locus_tag="KRH39_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBG8511811.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8108..10933
                     /gene="uvrA"
                     /locus_tag="KRH39_001909"
     CDS             8108..10933
                     /gene="uvrA"
                     /locus_tag="KRH39_001909"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG8511812.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11192..13009
                     /gene="uvrC"
                     /locus_tag="KRH39_001910"
     CDS             11192..13009
                     /gene="uvrC"
                     /locus_tag="KRH39_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBG8511813.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13009..13938
                     /locus_tag="KRH39_001911"
     CDS             13009..13938
                     /locus_tag="KRH39_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBG8511814.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14152..15948
                     /locus_tag="KRH39_001912"
     CDS             14152..15948
                     /locus_tag="KRH39_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBG8511815.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIEEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDIEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16088..17869)
                     /locus_tag="KRH39_001913"
     CDS             complement(16088..17869)
                     /locus_tag="KRH39_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG8511816.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17892..19778)
                     /locus_tag="KRH39_001914"
     CDS             complement(17892..19778)
                     /locus_tag="KRH39_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBG8511817.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19795..20292)
                     /locus_tag="KRH39_001915"
     CDS             complement(19795..20292)
                     /locus_tag="KRH39_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBG8511818.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20610..22091)
                     /gene="cls"
                     /locus_tag="KRH39_001916"
     CDS             complement(20610..22091)
                     /gene="cls"
                     /locus_tag="KRH39_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG8511819.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8510 a         2970 c         3698 g         6945 t
ORIGIN      
        1 gggtaaattt ataaaaatta aaaaattttt attttttatg tttaaacctt gtaaatttgg
       61 gacataatat aattgtaaaa cttaatatag caaaaataaa ttactcaatc tcccccactt
      121 aaagacctct atcccccata gaggtctttt ttactttcaa atcaaattat ataaaaaata
      181 aatagtttta tatgactctt cataaattaa aattacattg tgatagcaaa ccatgaatat
      241 gaaaaatata gaaaattata gctaacataa gctgtatatc atcttaacct cagtatattt
      301 aacaaaaaat gtttataatt taacctttat tttaataagt ttaccatata ttcactttgt
      361 cttaatatat cctttacaaa ttaagagtat tattaatcta taagtaaatc gtagaattaa
      421 attaaatatt ttgggaggat aaaaatggca gatataagac tagaaaatgt gacaaaatct
      481 tataataaaa aaaatacagt tatagaaaat ttaaatctta ctatagaaga tggttctttt
      541 acagtattgg taggcccaag tggatgtggt aaatctacta cgttaagaat gattgcaggt
      601 cttgaagata taacttcagg taggttgaag attgaagaaa atgatgttac aaaaacggat
      661 gctagtaaaa gggatatagc tatggtattt caaaattatg cattatatcc acatatgact
      721 gttagagaaa atatagaatt tggtcttaaa aacaaaaaag tagaaaaaga aaaaagagaa
      781 aaacttataa cagaagttat tgatgtagta ggattgagag aatatttaaa tgtgaaacca
      841 ggcaacttat ctggaggtca aagacaaaga gtggcacttg ctagagctat ggttaaaaat
      901 ccaaaggtat ttttaatgga tgaaccattg tcaaatttag atgctaaatt aagaaatcaa
      961 atgagaacag aattaataca attacatcaa aaactaaagt ctacatttgt ttatgtaact
     1021 catgaccagg ttgaggcaat gtcaatggca gataagatag ttataatgaa caaaggaaag
     1081 ataatgcaaa taggttcccc aaagaagata tatcaaaatc ctaaaaattt atttgtagct
     1141 caatttatag ggagtccttg tatgaacata ctagatataa aaaatgatat taaatttgga
     1201 ttcaggccag aaaaagcaac actagagagt ataaacatag atgggaaaaa tgatgatgta
     1261 tttagcctat ttggaaaagt tgtgactaaa gaagtgttgg gaaatgaaac tatatattgt
     1321 ttatctgttc ttaatagaga gataagagtc aaaacggaaa atgatatatt tgatattgga
     1381 agaactttaa gaataagtgt attagaaaaa gatttatatt attttgataa aaatggagaa
     1441 agaatagaag atgtaaaagt atgtaaaagt atgtattcta aaattaaagg agatatttat
     1501 gaaaaagcaa tttaataaag catatttata tataacacct gctattatag gaactttatt
     1561 gtttatagtc tatccaataa ttaagacgat atctctaagc ttcaaatctg gtagtcttct
     1621 tagttcaacc tttgaaaatg taggcttaaa caattataca gaacttttaa agaatccaga
     1681 gtttatacaa actataacga acactgcaat ttatactttt tttatggtga ctatatctat
     1741 atcactagcc atagtacttg ctgtttggct aaataaaaat tctataatac acaatttaac
     1801 gcaaagtata atctttacac cacatgtaat ttcactagta tctgtagctg tattgtggct
     1861 ttggattatg gagccacaat atggcttttt aaactggata ctatcgacat tacatttacc
     1921 aacatcaaag tggctttcaa gtgaaagttc agctttaatg tcactaattc taataggaat
     1981 atggaagact cttggatata atatcttaat agtgttatca ggtcttaaat ccataccaaa
     2041 ctatatatat gaagcatcta aattagataa tgcaaacaaa ataacgaatt tctttaaagt
     2101 gacattgcca ctattatcac caacaatatt ttttcttatg ataacaacaa caactgcatc
     2161 ttttcaagta tttgatgaga taagtataat gacacaaggt gggccagtgg gttcaactaa
     2221 catgttatct tattatatat atgagtctgg atttatatac tatgacatag ggcaagcgag
     2281 tgcagcgagt atgtttttgt tagctatagt tatgattgct acatatgcta actttagact
     2341 cttatcaaag aaagttcatt atcaataaag aaaaagtaga tattataagg tttatccaaa
     2401 aggaggagac aatggatatt atacaaccta tcaaaaaaga caaagttgat ataaaaaaaa
     2461 tagcatcaaa atcaatagaa atattaattc tattactttt atctgcaata tttatattcc
     2521 cttttatatg gatgctatct acatccttta aaaatccaca agaaatgatg caattaccgc
     2581 ccactattat accaaatgat atagtgttag aaaattatag tacagcatgg cattcaggtc
     2641 cattttttag atatttgcta aacagtgtat ttgtaacagg aagtgtttta attgtacaat
     2701 ttttagttat ggttccagca gcatatgcat tttctaagat taaatttaaa ggtgaaaaaa
     2761 tgctatttgg gcttttgctt gtaggactta tgattccacc acaagtaact tttctaccag
     2821 tctatatgat gatgagtaag tttggattta taaatactta tgttccactt ataattccat
     2881 ttatgacatc ttcatttggg atatttctgc ttagacagaa ttttatgcag atttcagatg
     2941 aaattattga agcagctaaa ctagataatg cttctgactt aaaaataatg tttagaataa
     3001 tggttcctat ggcaaaacca gcagtaataa catttatatt atttaatttt atataccatt
     3061 ggaataatta tttctggccc ttagtaatga caaatacaga tgcaataaga actttaccaa
     3121 taggagttgc gctccttaaa tcttcagaag gaattactgc atggaatgta ataatggcag
     3181 gaaatataat tctaatttta ccaataattc ttgtttatat atttgcgaat aaaaaagtaa
     3241 aagaagcttt tatgtattca ggaataaaat aaaaaattaa agactaaatt tggaggatga
     3301 aaatgaagct taaaagaaaa ttagcaacag gattagcaat aacattatta ttaggttctt
     3361 tagttgggtg ttcaaattct aatgatgcaa atgcatctaa aggagaaaag actgatagca
     3421 atattacgaa gatagatttc tgggctcctt taggtggaac taatggagaa actgctcaaa
     3481 aaatggttga ccaatttaat agtgaacata aggatgtaca agtaaatatg ttaaaacaga
     3541 aagattacta tgaaaatgca acaaaactac aggcagcctt aacatctaaa gaccaaccag
     3601 atgtaacatt attagaaata actcaaacag gtacattcgc atcagcaggt gctttagtag
     3661 atatgagtaa atattttgat aaaacatatc aagaaagatt cttttctggt cttttaacaa
     3721 attcatatta tgaggataag tttgttggta tgccatttaa tagaagtaca ccaatattat
     3781 acataaataa agatatggca actaaagcag gtcttgaccc ttcaggtcca aaatcatggg
     3841 aagagcttaa ggattatgct agtaaaatga caaataaatc agaagatact tatggttttg
     3901 aaacaccaat agatatttgg ttctatgaag ctatggtaat gcaaagtgga ggagaaataa
     3961 ctaatggtaa aaaagtagca tttaataatg aggcaggaca agcaccagtt aagttctggc
     4021 aagatatgat gaaagctgga ataatgaaaa tgcctcctgg agaagattat aatgcctggg
     4081 acgtagcaaa acaagatttt gtaaatggta aagtaggtat gatatttaca tcaacagcag
     4141 atttagcagg acttatgcaa caaacagaag gtaagtttga aatatcaaca gcattcttac
     4201 caaaaaatca aaaatacgca actcctacag gtggagctaa tctagttatg cttgaaggtg
     4261 gaacagataa agaaaaagaa gcaagtgcag aatttataga atggatgaca caaacagata
     4321 aaatcgttca gtttagttca tcaacaggat acttaccaac aactgaagat gcaacaaact
     4381 cagaaaagtt acaaacatta tataaagaaa aacctcaata taaggttgca actgaccaat
     4441 tacaatatgc tgttgcactt ccaatgttaa atggatacaa ggaagcaact gataagctaa
     4501 tggatgagat taaaaagggg cttactgatt taaatgcaaa tccaaaagat gtagtaagca
     4561 aaggaacttc tgcaatgcaa gcagtaatag ataaaacaaa taaataatta tatagacttg
     4621 taagttgatt aaaagtattc aataatatat aaatgaagag gtaatgtaaa tgacaaaggt
     4681 tatagtatta tcagtagatt ctttatttga aaaagacttg gaatttgtaa aaaatctacc
     4741 aaactttaaa tctatactag aaaattgctc catagtaaaa aatataaatt gtgtttatcc
     4801 cactctgaca tatccgtgtc atactagtat ggttacagga gtatatccta aaaaacatgg
     4861 tatatatcat aatgaaaaat ttgacccaaa taaagaaaat aaagactggt actggtactc
     4921 aaaggacata aaagcaaaaa ctattataga tgtagcaaaa gaaaataatc taactacctc
     4981 tagtgtatta tggcctgtaa tgggagcaaa tccaaatata gattataata ttgcagaaat
     5041 atgggctcct agtagagaag atgacccaag agaaactttt gaacgaagtt ccagtaaggt
     5101 tataatggac ggtatttaca atagacattg tcattatatc gattggaaat tagaacccaa
     5161 catggattta tttggagtaa actgttcagt agatataata aaggaatata aaccagattt
     5221 aatgctaata catttagcta cattggacca tacacgacac aattatggct tatttaatga
     5281 cgaagtagat aaagcattaa aaatgaatga taaatggcta ggagatatta ttcaggctac
     5341 aaaggatgca ggtacataca aagatacaaa ttttataata cttggggacc atggtcatct
     5401 tagagttgat aaagttataa atccaaatgt cttgcttaaa tcaaatggat ttataaaagt
     5461 tgaaaatgga gaagttaagg attttgatgc ctatgtaaat tcttctggaa tatcagctca
     5521 aataatagta aataacttag ataggttaac agagttaaga gaactgcttg aagaatttaa
     5581 agaagaatta ttcatagaga atatttttac caaagaggaa gcatctaacc ttggtcttga
     5641 aggtgatttt gaaatggtaa ttgaaggtct tgaaggaatt tcatttggaa atgatttcga
     5701 aggaagtatt ataaaagatt cagatataaa agattacaag tttgctgttg cgacacatgg
     5761 tcatttacct actaaaggta atagacctcc atttatagct tttggaccaa atattaaagg
     5821 tggtgtggtt atagaagaag gagacctgag agcacatgca gcaactatat taaaaatgtt
     5881 taatttaaaa ttagatggag ttgaaaaaga agcatttgat tttattatgt aagttgaata
     5941 aagtatttat tttccatgaa ttatagactt ctattgcaaa gatagaagtc tttttttatg
     6001 ttttagattt aagtaaaatt taaatgggta aaatatttaa aataacttaa acaattttat
     6061 aaaggtatga tataataata catgtcagga actaatgttc gaaaaaagga gtgatactat
     6121 ggattttaaa ataaaatcag acttcaaacc tacaggtgac caaccagaag ctattaaatc
     6181 aatagtggat tctataaata gaaatgagaa attttcgaca ttattaggtg taacgggttc
     6241 aggaaaaaca ttcacaatgg caaatatcat tcaacaagtt aagaaaccta ccctaataat
     6301 ggctcataat aaaactctag cagcacaact ttacagtgag tttaaagaat ttttcccaga
     6361 caatgcagtt gaatactttg taagttacta tgattattat caaccagaag catatgtagc
     6421 tcacagtgac acttatatag aaaaagatgc aagcataaat gatgaaatag ataaacttcg
     6481 acattctgct acagcatcta ttttagagag aagagatact ataataatat catctgtatc
     6541 ttgtatatat ggacttggtg acccaaaaga ctataaagaa cttatgttat ctataagacc
     6601 aggtatgcaa agagacagag atgatgtaat aaaaaggtta atagagatac aatacgaaag
     6661 aaatgacata aactttacac gaggtacctt tagagttaga ggagatatac tagaaatatt
     6721 cccagctagt aatgatgaaa aagcaataag aatagaattt tttggtgatg aggtagatag
     6781 aataacagaa attgattatg taacagggaa gattgttggt actagaaatc atgttgttat
     6841 attcccagca tctcactatg taacaacgcc agaaagaatt gaaaaggcaa ttgtagaaat
     6901 agaaaacgag cttcaagaac aaattaaatt tttcaaggaa aatgataggc tattagaagc
     6961 acaaagaatt gaacaaagaa ctaagtatga tatagaaatg ttaaaggaaa taggattttg
     7021 tcaggggata gagaactatt ctagacatat tacaggaagg tcagaaggag aaagaccata
     7081 tactttaatg gatttcttcc cagatgatta tttgataatt gtagatgagg cacatgtcac
     7141 tataccacaa gtaagaggta tgtatgcagg agacaggtct agaaaaacat cacttataga
     7201 gaatggtttt agattacctt ctgcacttga caatagacca ttaaattttc aagaatttga
     7261 aggaaatata aatcaaatgt tgtttgtaag tgctacacca gggccttatg aaattcaaca
     7321 ctcagagaca atagcagaac agataataag accaactggc cttttagacc caattgtaga
     7381 agtaagacct ataaataatc aaatagatga tttggtagga gaaataacta aaactataga
     7441 aaaaaatgaa agagtgctta taaccacttt aactaaaaaa atgagtgagg acttgactaa
     7501 ctatcttaaa gaaataggga taaaagttaa atatttacac tcagatatag ttacattaga
     7561 aagaacagaa ataataagag atttaagatt agggaagttt gatgtccttg taggaataaa
     7621 cttacttaga gaaggattgg atataccaga agtttcactt atagcaattt tagatgcaga
     7681 caaagaaggt ttcttacgtt ccgaaacagc tttaatacag actattggac gtgcagctag
     7741 aaatgaaaat ggaagagtaa ttatgtatgc agacaggata actgactcaa tgcaaaatgc
     7801 tattgatgaa acaaagagaa gaagagatat acaaaattta tataatgaag agcacaatat
     7861 cattcctaaa actatacaga aaaatataag agatagtata gaagctacta aggttgcaga
     7921 ggaagaagtt gtatatggaa tatctgatac agatgataaa gacgaaataa gagctaatat
     7981 agataaatta aaatcagaga tgatggaagc tgctcaaaac ttacaatttg aaagagcagc
     8041 agaacttaga gataaagtga aacaattaga agaaaaattg gaaaaataga aaggtaaaaa
     8101 caacattatg gaagataaaa ttataataag aggagcaaaa gaacataatt taaaaaatat
     8161 agatttagaa ttaccaagag ataaatttat tgtttttaca ggtttgtcag ggtctgggaa
     8221 gtcttctttg gcatttgata caatatatgc agaaggacag agaagatatg tagaaagtct
     8281 atctgcttat gcaagacaat ttttaggtca gatggaaaag ccaaatgtag aatatataga
     8341 gggtttatca ccagctatct caattgacca gaagactacc tcaaaaaacc cacgttctac
     8401 ggttggaaca gtaacagaaa tatatgatta tttgagacta ttatttgcaa gagtaggaga
     8461 tgtgtattgc cctacatgtg gtgaaaaaat aagtcagatg actattcaag aaatagttga
     8521 taaaatgcta gattttccag atagaacgaa attacaaatt ctttcaccaa ttgttagagg
     8581 acaaaaaggt acacataaaa aagtctttga taatatcaaa aaagaaggat ttgtaagagt
     8641 tagagttaat ggtgaaaact atgaagttag tgatgatata atcttaaata aaaatcaaaa
     8701 acataatata gaagttgtag tggacagaat agtaattaaa gatggaatag aatcaagact
     8761 agcagattca attgaaactg cagttaagct atctgatggt cttgtaatag cccaaataat
     8821 agatggagaa gaaattcttt tttcaactaa atttgcatgc ccagaacatg gaattggcat
     8881 agaagaatta tctccaagaa tgttttcatt taatgctccc tttggtgctt gtgatgtttg
     8941 taatggtctt ggggaaagta aagaagttga cccagaatta gtagtaccaa acaaagattt
     9001 atcaataaaa caaggtgcag tggcagcatg gggttcaaca ggtgttaatg atgacactta
     9061 ttacagtaaa atgataaaaa gtttagcaga gcattttaat gtatctttga caacaccgtt
     9121 taaagattta ccagatgatt ttgtggagga attactttat ggtaaagata atataattgt
     9181 agagtttact tatgagtcaa aatttggagg aactagaaat tataaatcat actttgaagg
     9241 tgtaatagta aacttagaga gaagatatag agaaactaat tcagattaca tgagagataa
     9301 gattgaagaa tacatggcag agagaccatg tccaaagtgt aaagggatga gacttaaaaa
     9361 ggaagtttta tctgttttag ttgatggaaa aaatataatg gaagttacaa acttatctgt
     9421 aaatgaattg attgacttta tggaaaatat aaatcttaca gaaaaacaga gatttatagc
     9481 tcatgagatt ataaaagaga taaaaggaag agctatgttc ttaagagatg taggtcttga
     9541 ttatttaaat ttatcaagaa aagctggaac tttatcagga ggagaagcgc aacgtataag
     9601 attagctact caaataggtt ctgcattggt tggagtttta tatgtacttg atgagccaag
     9661 tatagggctt caccaaagag ataatgatag gcttataact actcttagac atctaacaga
     9721 tttaggaaat accttaatcg ttgtagagca tgatgaagac acaatgagag aagctgatta
     9781 tgttatagac attggtccag gtgcaggtat acatggtggt gaaatagttg cacagggtac
     9841 tttagatgaa ataattgaaa atgagaactc aataacgggt caatatttaa gtggtaaaaa
     9901 acaaatattg cttcctgaga ctactagaga aggaaatgga agttttatag aaatagttaa
     9961 agcatcagaa aataatctga aaaatattga tgtaaagttc ccattaggta agtttacatg
    10021 cataacaggt gtttcaggtt ctggaaaaag tacattaata aacgatatac tttacaaagg
    10081 tgttgcaagt aaagtgaata gacttaagca aagagctggt aagcataagg agataaaagg
    10141 aattgaaagt atagataaag ttataaatat agaccaaagt cctattggaa gaacacctcg
    10201 ttcaaatcct gctacttata caggtgtatt tgattttata agagatattt ttgcatctac
    10261 taatgaagct aaagcaagag gttataaaaa aggtagattt agttttaatg taaaaggtgg
    10321 tagatgtgaa gcttgtaagg gtgatggaat aataaaaata gaaatgcatt tcttaccaga
    10381 tgtatatgtt ccttgtgaaa tttgtaaagg tgaaagatac aatagagaaa cattgcaagt
    10441 aaaatacaaa gataaaacta tatctgatat attagatatg aatgtggaag aagctgttga
    10501 gtttttcgaa aatataccaa atataaaaag aaaattagaa actttaatgg atgtaggact
    10561 ttcatatatt aaattaggac agccttcaac tcaattatca ggaggagaag cacaacgtat
    10621 aaaattagct gctgaactta gtaaaagacc aacaggaaag actttgtata tattagatga
    10681 acctactact ggacttcata tggcagatgt agacaagcta atccaagtat tacaaagact
    10741 agcagataca ggaaatacaa ttatagtcat agaacataat ttagatgtta taaagacttg
    10801 tgattatgta atagacttag gaccagaagg cggagataag ggtggaacta tagttgcaac
    10861 tggaacacca aaagaagtat ctaaagttga tggttcttat acaggtcaat tcttaaagaa
    10921 atatttttct taagttatat aaattgtgaa aaattttaga gcagatatac gataaactgt
    10981 tttgttataa gtaaattagt caaatataag ttgtaaatag taatacaaag taaagtccat
    11041 attactcttc atggttaaca ttgttttcaa atcaattata gaagggaata tgggctttaa
    11101 cttgtttaaa gcactggaaa ttgtataact ttttaaaatt catattttag ggtataattt
    11161 tagtataaaa tttaatgttt tggagggcga gatgtttgat atacaagagc atttaaaaaa
    11221 attaccttct gagcccggag tatatttaat gaaagacaaa tacgaccata taatatatgt
    11281 gggaaaagcc atatctctaa aaaatagggt aaggcagtat tttcaatctt caaaaaacca
    11341 tacatctaaa gtaaaatcta tggttaagaa tatatataag tttgagtata ttataacgga
    11401 ttcagagtta gaggcattaa tacttgaatg taatttaata aaaagataca gaccaaagta
    11461 taatgtagtg cttagagatg ataaaactta tccatatata aaagtaacta cgaatgaaga
    11521 ttatccaaga atcttaaaag ttagaagagt tcttaaagat aaggcaaagt attttgggcc
    11581 ctataccaat ataactgctg tgaatgatac cttggagctt ataagtagta cgtatccaat
    11641 aagaagctgt aaaatagata tagataaagc aataaagaat aagacaagac cttgtttaaa
    11701 tctacacata aataaatgtt taggaccttg tacaggaaat gtttctaaag aagagtatgg
    11761 aaaaatgata gaagagatta taatgtgttt atctggtaaa gaagaaaaat tgatggaact
    11821 tttaaaggaa aaaatgaatg agagttctat gaactttaga tttgaagagg ctgctgtata
    11881 tagggataag ataaagagcc ttgaagagat gatacagaaa caaaaaatag atgctactgt
    11941 aagtgactta aaccaagatg tggttgcaat ggcaagagct cataatgaag cttgtgtcca
    12001 ggtatttttt ataagaaatg gtaagattgt aggtagggaa cattttatat tagaaggggt
    12061 aatggatagt ccaagagctt ctatattaag ttcatttgta aaacaatttt ataatgaaca
    12121 agagtatata cctaaagaat taattataga agatgagata gaagatagtt ctatattaga
    12181 agaatggctg tcatctaaaa aggggcaaaa agtaacaata agagttcctc aaaagggaga
    12241 gaagaaaagc ttagttgaaa tggttaggaa aaatgctgta gaatatcttg aaaaattttc
    12301 tgatatgaat aaaagaaaat atgaaaaaag tattggagca ttggaagaat taaagcaaat
    12361 attaaatcta gaaaagttgc caataagaat agaggcttat gatatatcaa atatacaagg
    12421 tgtagattcg attggttcta tggtagtgta tacgaatgct aaaaaagata agaaggaata
    12481 taggagatat aaaataaaga cagttattgg acctaatgat tatgattcta tggctgaaat
    12541 tgtagataga agattaaaac atggaaattt acctgacttg atattgttgg atggtggaaa
    12601 aggacaagtt agtgcagtaa aaaaagtact agaactcaat gatgtagata tacctctttg
    12661 gggaatgtac aaagatgata agcatagaac taaaggattg atatgcaaag aaaaagaaat
    12721 agaactagat aaaacaacta atttatatag atttattgct agtatacaag aagaagttca
    12781 taattatgca ataacatatc atagaagtct aagaaataaa gctttgacaa aatctatctt
    12841 agatgatata caaggaatag gtgaaaaaag aaagaaatca ttattaaatc actttaaaga
    12901 tgtagatgct ataaaaaaag ctactatgga agaactttta gaagtagatg gaatgaataa
    12961 aagcattgca gataatgttt ataatttttt tagaaaggaa gaaaattaat ggaaactagc
    13021 aataaagtat caataagaga gataataaaa gagttgaagt taaaagtagt atacatgcct
    13081 gatgatgtag attattatgt ctactctcaa gatataaata gaccaggatt acagtttgca
    13141 ggatattttg attattttgc atatgaaaga atacaaatag ttggaaagac agaatataat
    13201 ttttttggat taatggatag tcatattcga gaagaagtac tagatagatt tttctcatat
    13261 gaaataccag cgttaataat ttctagagac ttggaaatca agccagatgt aattgaaaag
    13321 gctaaaaaat ataaaagaat acttttgagc acaaatagaa ataccactag acttataaat
    13381 agactgtcaa actatttgga taataagtta gcaccacaca caacaattca cggggtgcta
    13441 gtagatattt atggtatagg tgtactgata aaaggtgaaa gtagtatagg aaagtcggaa
    13501 acagcattag aactgattca aaggggtaat agattagttg cagatgatgc tgtagaaata
    13561 agaaaattag atgaaagtat gcttgtgggt cagtcacctg aactaataag acattttctt
    13621 gagataagag gaataggaat aatagatgtt agaagtttat atggagtagg ggcaatcaaa
    13681 aattctaaaa tgatagattt agtagttcat ttagaagctt gggatgaaac taagtattat
    13741 gatagactag gactggataa agaatatgaa gaaatacttg gtgttaatgt agagaaatta
    13801 gttgtacctg ttaaacctgg aagaaatact gctatgatat tagaagtagc agcaatgaat
    13861 tttagacaaa gaggaatggg atacgatgca gctcaagaat ttactaaaaa attatcaaaa
    13921 ttaatcgata ctaagtagta ctgaataata gtgaactaca catcatttaa ttaaatattg
    13981 attaggatta tatcctacac cttataaatt aaaatcaagt tgtatatata tgatgtttat
    14041 taataagata tattatataa ggataattta aagtacagtg ttagattata ataatactag
    14101 attaatttta tatttgagtt tcattatata acaagaaagt tggaattttt tatgggaata
    14161 gaatatttta gtaaactttt agaaagtagt actgaaaaag aattgctctt aagtaaaaag
    14221 tcaaataaca taagtacagc aagattaata tcttttttga tagtgatagc agggcttgct
    14281 attggatttt acaataaaaa tatagtaggt atttttgttg gagttttatc aataataata
    14341 tttatagcac tcttagttat tcatagtaaa gtaaaagaag aagaaacata ttttaaaagc
    14401 aagagcgaag tattaaataa gtatattaag agatttggag atggttggaa ggaatttaaa
    14461 atagatggaa aagaatactt aaaagatgaa aactcgcaag caaaagattt agacttattt
    14521 ggaagagcat cattgtatca atatatctgt gtagctaata catcttatgg gaaaaatttt
    14581 ttagccaaat atttgtggaa tgaaaatcca gatgagaatg taatcttaga aagacagaga
    14641 gctataaaag aacttttatc aaaagatgat ttttctattc atattcaaac actcagtaat
    14701 ataattggta aagagcaaaa aaacaattca gatagtagta tagaatcgtt tatagaatat
    14761 ggagaaaata agaatgtgta tataccaaaa tggatgcata tttttacttg gggtttacca
    14821 actgcaacaa tcttatcttt tatattttgt atgttaggat ttttacctgt tttacctgtt
    14881 tttttacttt ttataatcca gttagggttt agtggttttg gatatccaag acttatgcaa
    14941 actcttgctc cattattttc atttagtaga agtatacaag tatatgaaaa aatgtttgag
    15001 gttttagaaa aagagacgtt tgagagtgca tatttgaaag aattacaaga taaactttct
    15061 aagggaagtg gagtttcaag aggtgttaaa caattaaatt caataggaaa tgctgtaaac
    15121 ctaaggtata atcagatact atatataatt gcctgtggtg ttttgatgtg gaactatcat
    15181 tgtgcagaag ctttggagcg atggaaaggt atatatggaa atcaaattag agattggttt
    15241 gaatctattg gagaatttga agctctaata agtttaacag ttgtttctca tgtaaaggaa
    15301 aatacatgtt ttccagtaat aaaatatgaa gatactccaa gattgaaagt tgaagaggta
    15361 taccacccat taatagaaga aaaaagtgtc atagcaaatt cgatacaatt aaattctcag
    15421 acttgtatta tcacaggttc aaatatgtct ggaaagacaa catttttaag aagtatcggt
    15481 gtaaacttag tgctggcata tgcaggtgct ccagtatgcg ctaagagttt tgatgcgacc
    15541 tgtatggcta tatttacttc tatgagaata caagatgatg taagtcaagg aatatcaact
    15601 ttctatgctg agattcttag aataaaatca atgattcaat atagtttaaa agaactgcca
    15661 atgttggttc tagtagatga aatttttaag ggaactaact ctgctgatag aattattgga
    15721 gcaagtgagg cagtaaagaa attatcaaaa ccctgggtta taagtatggt gacaacacat
    15781 gactttgagc tatgtgacct ttcaggtagt ggagatattg aagttgtaaa ctatcatttc
    15841 tcagagtact atgtagacga taaaattcat tttgactata agataaaaga tggaagatgt
    15901 aaaactacaa atgcaaaaca acttatgaga atggcaggta ttctttaatt aatagatagg
    15961 ttaattaaag aataagtcta tttagaaata taatttaatt tgtgaaatta ataaggattg
    16021 tcttaaatta cgtaaaagta ttttaagaca atcctttttt aagaataaat tttatacttt
    16081 gttatgacta cttagcttct tcacaagcac attcttgtgg acactctcca tagacacaac
    16141 tcttatcaaa gtaatgagta tgaaggtatt tatgagctac atcactattt ggttctccaa
    16201 agaagtcttt ataaatagct tgtaaatcag gattctcatg agatttacga atagccttat
    16261 ttctatctat ttcatataac gcatttgcac gtctatcaac tatttcagta ttaccatggt
    16321 gataaggttg accagcacct ccaacacatc caccaggaca tgccataact tctatgatat
    16381 ggtatttaca ttctccactt ctgaccttat ccataatctt tctagcgttt ttaagtgtat
    16441 ttacaataca cacattaact gtagttccat caacatctat acttgcttcc ctaattcctt
    16501 taagacctct aacattagtg aagtttacat tatctaaagt cttattagtt atcttctcat
    16561 atgaagtacg tagggcagct tctaaaactc caccacttgc tccaaatata gatgcagcac
    16621 ctgtagattt tcctaaagga ttatcaaaat cttggtcttg aagattaggt aaatcgatag
    16681 cagcttcttt tatcatctta gctaactctc tagtagttat agaaaggtca acatctagta
    16741 ttccagattc acttaattct tctcttgaag cttcatattt tttagctaca caaggcatta
    16801 cagatacaac ataaagttct tcaggattga ttcctaatat tttaggtgca tagtagtttt
    16861 tagctataga accaaacata ccttgaggag acttacagct agatgctaag tttaacttgt
    16921 ctggataatt gtgctctaag aagtttaccc atgcaggaca acaacttgtt aatataggaa
    16981 gattttcacc tttttggatt ctttctataa attcagtagc ttcttccatt atagtaaaat
    17041 ctgcaccaaa gtttgtatca aatacatgtt caaatccaag tgcttttaaa gcagctacca
    17101 tttttcctgt agagattgac cctggttcta atccaaactc ttcaccaata gcaactctta
    17161 cagcaggagc aacttgcact actatagtct tttctttttt atttaaaaca tcccaaagtt
    17221 taggtacatt atctacttct gttaatgctc cagttggaca gacagatata cattgaccac
    17281 agaatgtaca ctctgtttca accatatcag agttaaagaa tgtactaact aaagtattaa
    17341 atccacgatt aactccagat aatgctccta ctgtttgtat gtcattacat acagtctcac
    17401 atcttctaca taatatacac ttagaatggt ctttaactaa agatttagta gaagtatctt
    17461 tacctgcaaa agatttagaa ccttgatatc ttatttttct tactcctaaa tcagcagcta
    17521 tagtctgtaa ttcacaatct ccattttttt cacagacaaa acaatcttgt ggatggtctg
    17581 ataacaataa ttcaacaata gttctacgag catttaatgc tttagcacta ttagtctgaa
    17641 ctttcatacc ttcctttata actgttccac aagctggaac taatcctctc tctgtctcaa
    17701 ccatacaaac acgacaagaa gcacaagtat caagcttatc tatttcattc atatgtaaat
    17761 gacaaaggtt gtgtattttt atatttatta atttcgcagc atctaagata gaagttccac
    17821 taggtgctga aacgtgtttg ccattaatag ttaaattaac taaactcatt gttattcctc
    17881 ctccaaataa gttatttctt gattatagcg ttaaatgtac aattatctat acaagcacca
    17941 cattttagac acttagaagt atctattgta tgttgttcct taacagaacc tgttatacaa
    18001 ccagcaggac acactctaga acattttgta caacccttac agtcatctgt tataaagtaa
    18061 gaaagtaagt tctgacattt accagcagga catttcttat ctacaacatg agctatatac
    18121 tcatctttga aatattgaag agtacttaaa acagggtttg gagcagcttt accaagacca
    18181 cataatgaag cagtttgtat agtttcagca aggttttcta ggtcatctaa atcttgtaga
    18241 gtaccattac cttctgtaat tttagttaat atttctaata atcttgtagt acctatacga
    18301 caaggtgtac atttaccaca agattcttca acagtaaact caaggaagaa cttggctata
    18361 tctaccatac agtcagattc atcaagaaca agcataccac cagagcccat cattgaaccg
    18421 atagaactta atgaaccaaa gtctatagga gtgtctaaat gttcagttgg tatacatcca
    18481 ccagatggtc cacctgtttg aacagcctta aatttcttac cttcagggat tcccccacca
    18541 atttcaaata caatatctct taaagtagta cccattggaa cttcaacaag tcctacattt
    18601 tctacttttc caccaagagc aaagacttta gtaccactag aatcttcagt acctaagttg
    18661 gcaaaccaat ctccaccctt aagtataatt gcaggtatgt tagcaaaagt ctcaacgtta
    18721 tttaagcaag taggagattt ccatagacca ctcttagaag aactataagt tttcattcta
    18781 ggttcaccac gtctaccttc aattgaatgc attagcgctg taccttcacc acaaacgaaa
    18841 gcacctgcac catattttaa ttctaattta aagttaaatc cagtgcctaa gatattttcg
    18901 ccaagtaaac cgtatttttc agcttgagct atggctactt ttaatctttc tatagatttt
    18961 ggatattctg ctcttatgta tatgtatcct gtatcagaac ctatagcgta tccacatatt
    19021 gccatagctt ctagtacact atgagggtca ccttcaagta cagatctatc cataaatgca
    19081 cctgggtcac cttcatcagc attacataca acaaactttt tatctgttgg accagctgga
    19141 ttttttgaag cagcttccca tttagagcca gtagggaaac ctgcaccacc tctaccacga
    19201 aggccagaag tttttacctc tgcaataact tcttgaggag taagagaagt taaacatttg
    19261 cctagagcta aataaccatc attggcaata tattcttcta tactatctgg gtctataaga
    19321 ccacaatttt ttaaagctat acgtaactgt tttttgtaga aagacatttc ttcttgttct
    19381 tcaacttttt tacttaaaga ttcttcctca tataataatt cctcgacaac tgtatttcta
    19441 attaagtggc tttgaactat cttttcagca tcagatggct caacttttac gtagaaaacg
    19501 ttatcaggat aaacttttac aataggacct tgagcacaga aaccaaaaca acctgtcaaa
    19561 cgaacactaa ctttatcttg gattcctgat ttctttattt cattttctaa ttcactaact
    19621 atttctagac tgtttgaaga tgtacatcca gtatcacaac aaacaagtaa ttctcttctt
    19681 aatacatttt ccttagaata attttttctt aagctaagat taggtttcag ctcatcagct
    19741 aaaactttta attcatcaaa tgaatttact ttacgcatta tttattcacc cctcttaaca
    19801 attcaattct aattctctat aagtatctaa tatttcgctt acttggtcag gtttcacttt
    19861 accataaact ttaccattta cagtaacaac tggagctaaa ccacatgccc ccaaacatct
    19921 taaaccttct aaagaaaatt taaaatcact agtagtttct ccaactttta tgccaagctg
    19981 tttttcaaaa gcgcttaaga ttttgtcagc accttttaca aaacatacag ttcctagaca
    20041 gacactgatt ttatacttac ctacaggttc tgtagtaaaa taagaataga atgtaacaac
    20101 cccatatacc tttgcaggag ctactccaag ttttctagca acatgaagtt gaacttcttt
    20161 tggaagatat ccaaatatac cttgggcttc atgtagaact tgtattaacg caccttcctt
    20221 tgttgcaaga gagtcaataa atatatctag ttcgtcaaat aattgcttgt tgtgtgaaat
    20281 aaaatcgcac ataacaaatc ccccttttct ttttaaagtt attatttttc ataaaataat
    20341 taaccatgag ttgaccccaa cattatgtgg gtgaacaaaa ccaaaacaat aaagaaagaa
    20401 taatatacaa caatttcttt atttataaaa acaatgcaaa aaagttacct attaaaaata
    20461 aaaactatac tagtctatta taatataaaa taattcagaa tagttaaaaa aaataaaaaa
    20521 aatgagctaa attcaataaa aaaaaaataa tacatataaa aaactttaaa aaataaacca
    20581 aaatgtaaaa tttttataaa ataaaagatt tataaaactg ctgataatag tcttgaaatt
    20641 gattccttga tttttatata agtagaccta gaattatata tatctaatgt tatttcctta
    20701 gaatttaaaa tatcattttc aaatataatt ctttgttcca atgctttttt tgaagagtac
    20761 ataaatgcat taacttcgaa attaagctca aaacttctga tatccatatt agctgttcct
    20821 atagagcata tagagtcatc tataactatt gtcttagcat gtaaaaatgc attttgacca
    20881 taagtataga ttttagctcc aaactttaaa agttctccag catatgaata tgatgcccag
    20941 tatacaaagg gatggtcagg tttagaagga atcataactc tcacatcaac accagataaa
    21001 caagctattt ttaaagtatc tataaaagtt gagtcaagta ttaaataagg actctgtatg
    21061 tatatatact ttctagcttt ctgaatcatt ttaaggtaac catatttaat ctcatcaagt
    21121 tctgatatat ctggaccact tgatactatc tgaatcccaa tattttcaga aggatgatta
    21181 gaatttgtat cttcttctat aaagtacttt tctaaatcta agtcttcttt agttgtatat
    21241 ctccaatcaa ggatgaatct catatttagg tcaattacgc aatcaccagt aagcttagta
    21301 tgtgtatctc tccatagacc aaattttgga tttctaccta aatattcatc accaacatta
    21361 tatccaccta caaatcctac cttaccatca atgacaacaa tttttcggtg atttctatag
    21421 tttaagttaa agtttacaat tttcaagaat gatgggaaga agcttccaac tttaacacca
    21481 ctttctttaa gtctggaaag tgtactattt tttagtgttc taccaccaac tgcatcaaat
    21541 aaaagtctta cttcaacacc ttctttagct ttgtctataa gaatgtctat tatttcagtt
    21601 ccgattttgt catctttgaa aatgtaaaat tgtatgttta tatatttttt agctttttta
    21661 agttcctcaa gaagactgtt aaaaaattga ctagattcag cataaatcca cacatcgtta
    21721 ttattggtat aatgagcatt atttgaatta gccaatgcat aaatcatatc cttatgttga
    21781 tgtatatctg aatcaatttc agatgtagat tggagtttaa cctgagtatc tagtatatta
    21841 cttttgatta tcttatcatc cttttctttc aatctaaaca tattgttttt tgaaatattt
    21901 ctgccaaaag ctatataaag tatgaatcca agagcaggaa tcaatactaa tataagaagc
    21961 catgttaaag ttgtatttat gtttcttttt tctctaaaga taaggtttag tataaccatg
    22021 aaattaatta tatatattgt agtaactgct atttcatata ttgacatttc gaaaaatgat
    22081 atatcaaaca taaaattccc tccttcaaat atttattaaa ata
//
LOCUS       DAEORZ010000055        21993 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEORZ010000055 DAEORZ010000000
VERSION     DAEORZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21993
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.055"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            386..697
                     /gene="rpsJ"
                     /locus_tag="KRH39_001917"
     CDS             386..697
                     /gene="rpsJ"
                     /locus_tag="KRH39_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBG8511820.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            789..1418
                     /gene="rplC"
                     /locus_tag="KRH39_001918"
     CDS             789..1418
                     /gene="rplC"
                     /locus_tag="KRH39_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBG8511821.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1448..2071
                     /gene="rplD"
                     /locus_tag="KRH39_001919"
     CDS             1448..2071
                     /gene="rplD"
                     /locus_tag="KRH39_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBG8511822.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2071..2361
                     /gene="rplW"
                     /locus_tag="KRH39_001920"
     CDS             2071..2361
                     /gene="rplW"
                     /locus_tag="KRH39_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBG8511823.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTASFKKAVVKLTADSKEIEFFQGM"
     gene            2391..3221
                     /gene="rplB"
                     /locus_tag="KRH39_001921"
     CDS             2391..3221
                     /gene="rplB"
                     /locus_tag="KRH39_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBG8511824.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3256..3537
                     /gene="rpsS"
                     /locus_tag="KRH39_001922"
     CDS             3256..3537
                     /gene="rpsS"
                     /locus_tag="KRH39_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBG8511825.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3569..3904
                     /gene="rplV"
                     /locus_tag="KRH39_001923"
     CDS             3569..3904
                     /gene="rplV"
                     /locus_tag="KRH39_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBG8511826.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3927..4742
                     /gene="rpsC"
                     /locus_tag="KRH39_001924"
     CDS             3927..4742
                     /gene="rpsC"
                     /locus_tag="KRH39_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBG8511827.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4778..5209
                     /gene="rplP"
                     /locus_tag="KRH39_001925"
     CDS             4778..5209
                     /gene="rplP"
                     /locus_tag="KRH39_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBG8511828.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5211..5414
                     /gene="rpmC"
                     /locus_tag="KRH39_001926"
     CDS             5211..5414
                     /gene="rpmC"
                     /locus_tag="KRH39_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBG8511829.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5438..5692
                     /gene="rpsQ"
                     /locus_tag="KRH39_001927"
     CDS             5438..5692
                     /gene="rpsQ"
                     /locus_tag="KRH39_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBG8511830.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5718..6086
                     /gene="rplN"
                     /locus_tag="KRH39_001928"
     CDS             5718..6086
                     /gene="rplN"
                     /locus_tag="KRH39_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBG8511831.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6110..6415
                     /gene="rplX"
                     /locus_tag="KRH39_001929"
     CDS             6110..6415
                     /gene="rplX"
                     /locus_tag="KRH39_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBG8511832.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6445..6987
                     /gene="rplE"
                     /locus_tag="KRH39_001930"
     CDS             6445..6987
                     /gene="rplE"
                     /locus_tag="KRH39_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBG8511833.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            7005..7190
                     /locus_tag="KRH39_001931"
     CDS             7005..7190
                     /locus_tag="KRH39_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBG8511834.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7223..7621
                     /gene="rpsH"
                     /locus_tag="KRH39_001932"
     CDS             7223..7621
                     /gene="rpsH"
                     /locus_tag="KRH39_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBG8511835.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7652..8194
                     /gene="rplF"
                     /locus_tag="KRH39_001933"
     CDS             7652..8194
                     /gene="rplF"
                     /locus_tag="KRH39_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBG8511836.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8230..8598
                     /gene="rplR"
                     /locus_tag="KRH39_001934"
     CDS             8230..8598
                     /gene="rplR"
                     /locus_tag="KRH39_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBG8511837.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8619..9128
                     /gene="rpsE"
                     /locus_tag="KRH39_001935"
     CDS             8619..9128
                     /gene="rpsE"
                     /locus_tag="KRH39_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBG8511838.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9143..9328
                     /gene="rpmD"
                     /locus_tag="KRH39_001936"
     CDS             9143..9328
                     /gene="rpmD"
                     /locus_tag="KRH39_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBG8511839.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9361..9804
                     /gene="rplO"
                     /locus_tag="KRH39_001937"
     CDS             9361..9804
                     /gene="rplO"
                     /locus_tag="KRH39_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBG8511840.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9848..11116
                     /gene="secY"
                     /locus_tag="KRH39_001938"
     CDS             9848..11116
                     /gene="secY"
                     /locus_tag="KRH39_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBG8511841.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11133..11783
                     /locus_tag="KRH39_001939"
     CDS             11133..11783
                     /locus_tag="KRH39_001939"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBG8511842.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11784..12530
                     /gene="map"
                     /locus_tag="KRH39_001940"
     CDS             11784..12530
                     /gene="map"
                     /locus_tag="KRH39_001940"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBG8511843.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGVSTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12552..12821
                     /locus_tag="KRH39_001941"
     CDS             12552..12821
                     /locus_tag="KRH39_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBG8511844.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12859..13077
                     /gene="infA"
                     /locus_tag="KRH39_001942"
     CDS             12859..13077
                     /gene="infA"
                     /locus_tag="KRH39_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBG8511845.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13113..13226
                     /gene="rpmJ"
                     /locus_tag="KRH39_001943"
     CDS             13113..13226
                     /gene="rpmJ"
                     /locus_tag="KRH39_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBG8511846.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13379..13750
                     /gene="rpsM"
                     /locus_tag="KRH39_001944"
     CDS             13379..13750
                     /gene="rpsM"
                     /locus_tag="KRH39_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBG8511847.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13786..14184
                     /gene="rpsK"
                     /locus_tag="KRH39_001945"
     CDS             13786..14184
                     /gene="rpsK"
                     /locus_tag="KRH39_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBG8511848.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14216..14839
                     /gene="rpsD"
                     /locus_tag="KRH39_001946"
     CDS             14216..14839
                     /gene="rpsD"
                     /locus_tag="KRH39_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBG8511849.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14917..15864
                     /locus_tag="KRH39_001947"
     CDS             14917..15864
                     /locus_tag="KRH39_001947"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBG8511850.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15885..16226
                     /gene="rplQ"
                     /locus_tag="KRH39_001948"
     CDS             15885..16226
                     /gene="rplQ"
                     /locus_tag="KRH39_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBG8511851.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16346..17179
                     /locus_tag="KRH39_001949"
     CDS             16346..17179
                     /locus_tag="KRH39_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG8511852.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17167..18033
                     /locus_tag="KRH39_001950"
     CDS             17167..18033
                     /locus_tag="KRH39_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG8511853.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18027..18830
                     /locus_tag="KRH39_001951"
     CDS             18027..18830
                     /locus_tag="KRH39_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBG8511854.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18862..19593
                     /gene="truA"
                     /locus_tag="KRH39_001952"
     CDS             18862..19593
                     /gene="truA"
                     /locus_tag="KRH39_001952"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG8511855.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19713..20144
                     /gene="rplM"
                     /locus_tag="KRH39_001953"
     CDS             19713..20144
                     /gene="rplM"
                     /locus_tag="KRH39_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBG8511856.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYREMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20173..20565
                     /gene="rpsI"
                     /locus_tag="KRH39_001954"
     CDS             20173..20565
                     /gene="rpsI"
                     /locus_tag="KRH39_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBG8511857.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            21023..21775
                     /gene="cwlD"
                     /locus_tag="KRH39_001955"
     CDS             21023..21775
                     /gene="cwlD"
                     /locus_tag="KRH39_001955"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBG8511858.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT         8835 a         2565 c         4508 g         6085 t
ORIGIN      
        1 caataataga gtaattcttt actaaactaa tggatttaat ataaaaaaag aggggggtaa
       61 ctcctctctt ttttgttttt tgttgacaag tagcgctaaa gcatatatac taaactagtg
      121 ccttgagaca gattaacaat caagtttaag gttatgatgt tgtaatagaa aataaaattg
      181 aaaaaaacag aaaaaaaact tgaaaaaatg taacagatga tataaaatat aatagtgtgc
      241 ttgagagatt aagaaaataa taattaaatc ctagtgaaat acgaactgat atcgatttaa
      301 aagttacaac acacccggag cagtgtatcg gtattggttg tacgcaactt gaaaattaaa
      361 cacgtgataa aggagggaaa acaaaatggc taagaatgaa aaaataagaa taagattaaa
      421 atcatatgat cacaaattat tagatttttc agcaggtaaa atagttgaaa ctgctaaaaa
      481 ggctggttca caagtttcag gacctgtgcc tctaccaaca gaaaaacaag ttgtaactat
      541 attaagagct gtacataagt acaaagactc tagagaacaa tttgaaataa gaactcataa
      601 aagactaatc gacatagcta atccaacacc taagacagtt gactcattaa tgagattaga
      661 cttaccagca ggtgtagata tagaaataaa attataagaa cccctaagat attatcctat
      721 agatgattat cttagaatga ttactctaaa agatgtaatc cgctgtaaga attataggag
      781 gtgctaatat gaaaggaata ttaggcaaaa aagttggtat gactcaaata ttcactgata
      841 aaggtgtagt tatacctgta acagctgttg aagcagggcc tatggtcgtt actcaaataa
      901 aaacagttga taaagatgga tacaatgcta tacaaatagg ttttgaagat gctaaagaaa
      961 aagctttaaa caaacctaaa aaaggacatt tagctgctgc taatgtttta aagaaacatt
     1021 taaaagaatt tagagtagat tctgtagaag gatacacagt aggacaagaa ataaaagctg
     1081 atgtatttga agcaggtgca aaaatagatg ttactggaat aagtaaaggt aagggattcc
     1141 aaggtccaat aaaaagacat ggacaaagta gaggtcctga aactcacggt tctagatacc
     1201 acagaagacc aggttcaatg ggagcttgtt cttacccagg tagagtattt aaaaacaaaa
     1261 aattagctgg acacatggga agcgtaaagg taacagttca aaacttagag gttgttaaag
     1321 tagatgctga caagaacctt atattagtta aaggagctat accaggagct aaaggttcag
     1381 tagtaactat aaaagaagct ataaaggttt ctaaataatg actaacctag agaaaggagg
     1441 aataacaatg ccaaaattaa atgtactaaa tgttagtgga caaaacgttg gagaaataga
     1501 attatcagat tctatatttg gcgtagaagt taatggacat gttttatacg aagttgtaaa
     1561 aaatcaatta gcaaataaga gacaaggaac tcaatctgct aaaactagag cagaagttag
     1621 aggtggcgga agaaaacctt ggaaacaaaa aggaacaggt agagcaagac aaggttctac
     1681 aagatctgta caatgggtag gtggaggagt tgcttttgca cctaaaccaa gaagctacaa
     1741 atatacatta cctaagaagg taagaagatt agctatgaag agtgctttat cttcaaaagt
     1801 tcaaaatagt gaagttatag tattagatgc attaaacatg gatgctccta agactaaaga
     1861 atttgctcaa atattaaaca atataaatgc tgctaaaaaa gctttagtag ttatagctga
     1921 caaaaacgat aacgtaataa aatcagctag aaatatagaa ggtgttcaaa ctgcattagt
     1981 taatactatg aatgtatatg atatattaaa atacgattca tttataataa ctacagatgc
     2041 tgttaaaaaa gtggaggagg tgtacgcata atgactaatc cacatgatgt aataataaga
     2101 ccagttgtaa ctgagcacag tatggctgaa atgggtgaaa aaaaatacac ttttgttgtg
     2161 gctaaagatg cgaacaaaac tgaaataaaa aaagcagtag aaaaagtatt cggagttagt
     2221 gttgataaag taaatacttt aaactacgat ggaaaagtta aaagaatggg tagaactcaa
     2281 ggaagaacag caagctttaa gaaagcagta gttaaattaa ctgctgatag taaagaaata
     2341 gaattcttcc aaggaatgta gtattataac aaacgaagga gggaaaacag atggctataa
     2401 aaaagtttag accaacttct cctgccttaa gacaaatgac agttttagtt tctgatgaaa
     2461 taacatgtaa tcaaccagaa aaatctcttt tagttaactt aaagaaaaat gctggtagaa
     2521 acgtacatgg tagaataact gttcgtcata gaggtggagg acaaaaaaga aaatatagaa
     2581 taatagattt taaaagagat aaagatggta tacctgctaa ggttgctact atagaatatg
     2641 atccaaacag aactgcaaat atcgcattat taaactatgc tgatggagaa aaaagatata
     2701 tattagctcc agttggaatt aatgttggag atactatatt atcaggatta ggtgctgata
     2761 taaaaccagg aaactgttta gcattaaaag atatgccagt tggtactatc attcataata
     2821 tagaattaaa gccaggaaaa ggagctcaat tagttagatc tgctggagtt tctgcacaat
     2881 taatggctaa agaaggaaaa aatgctttat taagattacc atcaggtgag atgagattag
     2941 taagcataaa ctgtaaagct actataggtc aagttggaaa tatagagcat ggtaacgtag
     3001 ttatcggtaa agcaggtaga aaaagacata tgggaataag acctactgta agaggttctg
     3061 taatgaaccc taatgaccat ccacacggtg gaggggaagg tagatctcca ataggtagac
     3121 catctcctgt tactccatgg ggtaaaccag ctcttggata caaaactaga aagaaaaata
     3181 aagcttcaaa taaactaata gtatcaagaa gaactaagta gtaaatttat ttttgctcgg
     3241 aaaggaggaa tataaatgtc aagatcaact aaaaaagggc cttttgtcca tgcaagactt
     3301 ttaaagaaga tagaagctat gaatgctagt ggaaataaag aagttataaa aacttggtca
     3361 agatcttcaa ctgtatttcc acaaatggta gaaaacacta tagctgttca tgatggaaga
     3421 aaacatgttc cagtatatat aacagaagat atggttggac ataaattagg tgagttcgtt
     3481 cctactagaa ctttcaaagg acataaggat gacgaaaaat ctaataagag aaaataatta
     3541 aattatgact aagaaaggag gaataacaat ggaagcaaaa gctactgcta aatacgtacg
     3601 tgtatcacca agaaaagcag gccaaatatg tgaccttgtt agaggaaaga atgttgatga
     3661 agcattagca atattaaagt ttactccaag aggagcagct tcaataatag ctaaggttgt
     3721 aaaatcagct aaagctaacg cagaaaacaa tcacgaaatg gatactgaaa aattatatat
     3781 agcatcaata gttgctaatc aaggaccaac aatgaagaga ttcatgccta gagctatggg
     3841 tcgtgcaact acaataagaa aaagaacttc tcatatagag gttgttgtta aagagaaaaa
     3901 ataattaata gataggaggg aaagtaatgg gtcaaaaggt taatccacac ggcttaaggg
     3961 tcggtgttat aaaagattgg gactcaagat ggttcgctac tgataaaaaa gagttcggaa
     4021 acttattatt agaagaccat aatatacgta aattcttaaa gaaaagatta tactcagctg
     4081 gagttgctaa gatagaaata gaaagatcag ctaacaaaat aaaaatggac ttacacgttg
     4141 ctaagcctgg tgtagttata ggaagagctg gtgcaggaat cgaagcatta aaagctgaat
     4201 tagaaaaaat gacaaagaaa actataatag ttaatatagt agaagtaaga agtacagata
     4261 aaaatgctca attagtagct gaaaatatag ctttagctat agagagaagg gttgctttca
     4321 gaagagcaat gaaacaagct atacaaagag caatgaaatc tggtgctaaa gggataaaag
     4381 tttctgcttc aggaagatta ggtggagctg aaatggctag aactgaaggt tatagtgaag
     4441 gaaatgtgcc actacaaact ttaagagcag atatagatta tggtttcgct gaagctgata
     4501 caacttatgg aaaaatcggt ataaaagttt ggatatgtaa tggtgaagtt ttaccaacta
     4561 gagacggtgt aaatccaaga gaagaaagca gaaagagcga tagaagagac aacaagagag
     4621 ataatagaag aaacgataga agaggaaatg acagaagagg aaatgataac agaggaaact
     4681 acagaggaca aagacctcaa ggtggatcaa gacctcaaag aactgaaaac aaaggaaatt
     4741 aataatagtt aggttcaagg aaggaggaaa aatactcatg ttaatgccaa aaagagtaaa
     4801 gcgtcgtaga gttcatagag gaagcatggc tgggcaagct cataagggta ataaagttac
     4861 ttatggtgag tttggattag tagcattaga agcttcttgg ataacttcta atcaaataga
     4921 agctgccaga atcgcgatga ctagatatat aaaaagaggg ggaaaggttt ggataaaaat
     4981 attccctcat aaaccagtaa caagaaaacc agcagagact cgtatgggtg cagggaaagg
     5041 ttctccagaa tattgggtag ctgtagttaa gccaggaaga gttatgtttg aattagcagg
     5101 tgtttctgaa gataaagcaa gagaagctat gagacttgct gcacataaat taccaatcaa
     5161 atgtaaattt gttaaaaaag aagatttaga agtaaagggt ggtgaatagg atgaaagcta
     5221 aagaattaag agatttaaca agcgaagagc taatgaacaa attaaacgac ttcaaaagtg
     5281 aattatttag cttaagattc caattagcta ctggtcaatt agaaaataca gctagaataa
     5341 agtttgttaa gaaggatata gcaaaagtta aaactgttct tgctgaaaga aagttatacg
     5401 aaactagagc ttaatttgga aaggaggctt tttaaacatg gaaagaggaa gaagaaaagt
     5461 tagaataggc cgtgttgtta gtgataagat ggataaaaca atagtagtag ctgttgaaga
     5521 attcgtacgt catccactat ataataaacg tgttaagaga actaagaaat tcaaggctca
     5581 tgatgaaaag aatatatgta acatcggaga tagagttaaa ataatggaaa ctagaccatt
     5641 atctaaagat aagagattca gactagttga agttgttgag aaagttaagt agtttttgaa
     5701 gaaggaggga ttacgatatg atacaacaag aatcacgtct aagagttgct gataactcag
     5761 gagctaaaga acttttatgt atacgtgttc taggcggaag taaaagaaga tatggtaaca
     5821 taggcgacgt tatagttgct actgttaaaa gtgcaacacc tggtggagtt gtaaaaaaag
     5881 gtaaggttgt taaagctgtt atagtaagaa gcaaacaagg cgtaagacgt aatgacggaa
     5941 gctatatatc ttttgatgaa aatgctgctg ttataataaa ggacgataaa actccagtag
     6001 gaactcgtat attcgggcct gttgctagag agttaagaga caatgaattc atgaaaatag
     6061 tttctcttgc tccagaagta ctataattaa ggaggtgcaa taggacatga tgcgtgttaa
     6121 aaaaggtgac actgttgtag ttatagcagg taaagataaa ggtaaaaaag gttcagtttt
     6181 aaaggtatat cctaaaacta gcaaagtgtt agttgaaggt gtaaatgtaa taactaaaca
     6241 tcaaaaacca agtgctatga accaacaagg tggaataata aataaagaag ccccaataca
     6301 catatctaat gtaatgccat ttgatcctga aacaggaaaa ggcgttagag taagatatga
     6361 agtaaaagat gggaacaaag taagagtatc agcaaagagc ggaaaagaat tataataaat
     6421 attgcttgaa aggagggacc ttaaatggct tctagattac aagaaaaata catgaaagaa
     6481 gttgctcctg ctttaatgga gaaatttgga tacaaaaacg taatggagat acctaagtta
     6541 aataaaatag ttattaacat gggtataggt gacgctagag aaaatccaaa aggattagaa
     6601 aaaggcgttg aagaattaga aatgatatca ggacaaaagc ctgttataac taaagctaga
     6661 aaatctgttg ctaacttcaa attaagagaa ggaatgccaa taggaacaaa agttacatta
     6721 agagctgaca aaatgttcta ctttatggac aaattagttt cagtttcttt accaagagtt
     6781 agagacttta gaggagttaa ccctaatgct tttgatggta gaggaaatta tgctttagga
     6841 gttaaagaac aattaatatt ccctgaaata gaatatgata aaatagataa agtaagagga
     6901 atggatataa tatttgttac tacagcaaaa actgacgaag aagctcgtga attattaaaa
     6961 ttattaggaa tgccattttc taagtaagca aaggagggat tcccgtggct agaaaagcga
     7021 tggttgttaa gcaacaaaga aagcaaaagt acgctactag agaatacact agatgtacta
     7081 tatgcggaag accgcattca gttttgaaaa aatttggtat atgccgtata tgctttagag
     7141 aattagctta taaaggtcaa atacctggtg ttagaaaagc aagctggtag aagcttgatt
     7201 agaatggaag gagggcaaca aaatgacaat gacagatcca attgcagata tgcttactcg
     7261 tataagaaat gctaatgttg ttaagcatga aactgtagat gttccagctt ctaatatgaa
     7321 gaaagaatta gctagaatct tattagaaga aggtttcata agaggttatg atgttataga
     7381 agatggaaaa caaggtatca taagaataca attaaagtac ggacaagaag gcgaaagagt
     7441 tataacaggt cttaagaaaa tatctaagcc tggaatgaga gtttacgctg ctaaccatga
     7501 gataccaaaa gtattaaacg gattaggaat atcagttata tctacttcaa aaggaatatt
     7561 aactgacaag caagctagaa aagaaaatgt tggtggagaa gtaatctgct acgtttggta
     7621 attaatatga acaatgtaag gaggtgcgac catgtcaaga ataggtgtta agccaataat
     7681 aataccagca ggtgttgaag ttactatagc tgaaggaaat ttagttacag taaaaggtcc
     7741 aaaaggaact ttaactaaac aattaagtgc tgaattaaac ataaaaaaag aagaaaatac
     7801 aataatggtt gaaagaccaa ctgataataa aaaacataga tctttacacg gactaactag
     7861 aactttatta gataatatgg ttgtaggtgt aaatactggt ttcgaaaaga aattagaatt
     7921 aaaaggtgtt ggatatagag ctcaaaaaca aggaaagaaa ttagttatga acttaggttt
     7981 ctctcatcca gttgagatgg aagatccaga aggaataact gttgaagctc caaaccaaac
     8041 agagttaata gtaaaaggta tagacaagca attagtaggg aattatgctg ctaagataag
     8101 agcttggaga aagccagagc catacaaagg taaaggtata aaatacgttg atgaagtaat
     8161 cagacgtaaa gaaggaaaaa ctggtaaaaa ataataccaa aacttaaacg ctagaaagga
     8221 gtgagctagg tgttaaaaaa ggctgataaa aatgctaata gacttcaaag acataagaga
     8281 gttcgtagaa aaataagcgg tacttctcaa agaccaagat tatgtgtatt tagaagtgct
     8341 aacaatatat atgctcaaat aatagatgac acaaaaagag taactctagt tgctgcatct
     8401 tctttagaag cagaagttaa gagtgctgtc aatcatacag gaaacaagga agcagctaag
     8461 aaagttggag aattagttgc taaaaaagct gttgaaaaag gaatcactga agttgtattt
     8521 gacagaggtg gatacttata tcatggaaga attcaagaat tagctgaagg tgctagagaa
     8581 gctggtctta agttctaata cgaaggaggg aaaaatacat gctacgtcgt aagccgatag
     8641 atgcaggaca acttgactta caagaaaaag ttgttgaagt aagacgtgtt actaaggttg
     8701 taaaaggtgg tagaaatttc agatttgcag cattagtagt tgttggagat gaaaacggac
     8761 acgttggtat aggtgctggg aaagcaatgg aagtaccaga cgctataaaa aaagcagttg
     8821 aagatgctaa gaaaaatcta atagtagtgc caatagttgg gactacaatt cctcacgaag
     8881 tacgcggaca ctttggtgct ggaaatatat taataatgcc tgctgtagaa ggtacaggag
     8941 taatagctgg gggacctgct agagccgtac ttgagctagc tggactaaaa gacgttagag
     9001 ctaaatcttt agggtctaac aatccaagaa atatggtaaa tgctacaata gaaggactta
     9061 attctctaaa aacagttgaa gatatagcta aacttagagg taaaaaagtt gaagaacttc
     9121 tagggtaagg aggtagtgta aaatggctaa attacaaata aagttagtta gaagtgttat
     9181 aggaactact cctaaccaaa aaaagaatgt tgaagcgtta ggattaagaa aaagagaaca
     9241 agttgttgta aaagaagaca atgctcaaac aagaggtatg ataaataaag taagtcactt
     9301 attagaagta actgaaatag ctgaataatt tttataaagt agtgcaagga ggtgcaatcc
     9361 atgaagttac atgagttaaa acctgctgaa ggtgcagtaa gagctaagag aagattaggt
     9421 agaggtactg caactggaca aggtaaaact gcaggacgtg gacaaaaagg tcaatggtct
     9481 cgttctggtg gtggagtaag agtaggattt gaaggtggac aaatgccact agctagaaga
     9541 cttcctaaga gaggtttcaa taacatattt aagaaagttt acacagaagt taatgttgaa
     9601 gttttaaaca gatttgaaaa tggaacagaa ataactgcag aattattaaa atctactaaa
     9661 actataagca aaataggtaa agacggaatt aaaatcttag gtgaaggtaa tttagagaaa
     9721 gctttaactg ttaaagctgc taaatttaca gcttcagctc aagaaaaaat agaaaaagct
     9781 ggtggaaaag cagagttagt ataataacct gctaatctcc tagccagtac aggcggggtg
     9841 aattagcgtg ctgtcaaaat taaaacaagc ttggaagatt aaagcagtaa gaaaaaaagt
     9901 aatgtataca cttatgatga tcgtgatatt cagaataggt actactatac cagttccagg
     9961 catagacact agtattatac aaaagatggt aggcggcaat agtctacttt ctctgtataa
    10021 tatgtttact ggtggagcat ttagtaactt ttcactattt gctttaggta taagtccata
    10081 tatcactgca tcaatcataa tacaacttct aacagtaggt tttgaaagtt tagcagaact
    10141 tcaaaaatct ggtgaagaag gaaagaaaaa aatcaataag tacactaaat atacagcact
    10201 agctctagca gttgtacaag ctttgggaat tacattagga attgtaagaa gtgctttaat
    10261 atcaaatagt gtgtttttta tcacaactgt tgtaataaca ttagtttcag caagtatgtt
    10321 agtaatgtgg attggggaca aaattactga aaaaggaatc ggtaatggta gttcagtaat
    10381 aatatttgca ggtataatat caagaatacc tacagatgtt attaaaatat cgcaacaagt
    10441 taaatctggc gaagttgcac cttgggttat agtgattcta gctgtagtta tattacttac
    10501 agtaacagga gttacattta tacaagaagc aactagaaag atacctgtac aatatgctaa
    10561 aagagttgtt ggaagaaaaa tgtatggagg acaaagttct catataccaa tgaaagttaa
    10621 ccagtcagga gttatgccta taatttttgc tagttcactt ttagctttcc ctcaaacaat
    10681 agctatgttt atgggtccaa atgctcaggc ttttgttcaa aaatacttaa gtatggcaac
    10741 agaacaagga ttttggacat atagatcaat tgagattcta cttataattt tcttctcata
    10801 tttttataca actgtatcat ttaatacaga agacatatct aaaaatatga aaaataatgg
    10861 tggatttata ccaggtataa gaccaggtga gcctactatg gattatttaa atagaatttt
    10921 aactagatta actttagctg gagctacttt cttagcaata attgctatgg tgccagctct
    10981 tacaacacat tatatgaaag taaatatgag tctagctggg acttcattgc taatcgttgt
    11041 tggagttgct ttggagctta agagacagtt agagtcaaac ttagttatga gaagctatca
    11101 aggtttctta aaataaaatg gagatggata atatgagaat aatattactt ggacctccag
    11161 gtgctggtaa aggtactcag gcggcaggaa tagtagaaaa atacaatata cctcatatat
    11221 caacaggaga tatattcaga aagaatataa aagagggaac agaacttgga aaaaaagcta
    11281 aagaatacat ggaccaaggt ttattagtac cagatgagtt aactgtaggt ttagttactg
    11341 atagaatatc tcaagaagat tgtaaaaatg gatttatgtt agatggattt ccaagaaatg
    11401 tagcacaagg agaacattta gatatcttct taaaaaatgc tggtatatca ctagataaag
    11461 ttgtcaatat tgaagttgat aagagtatat tagtgtctag agcagttggt agaagaatat
    11521 gtaagtcttg tggagctact taccatgttg agtttaatcc tcctaaagta gaaggtgtat
    11581 gtgatgtatg ccaaggagaa ttatatcaaa gagctgatga taatgaagaa actgtatcta
    11641 agagaataca agtttatcta gatgaaacta agcctttagt agattattat agcaaacaag
    11701 gtataatagc agatataaaa ggtgatcaag caatagataa agtatttgaa gatattgtcg
    11761 cagccttagg aagtggaaaa taaatgatta tcttgaaatc aaaaaaagaa atagaacttt
    11821 taagagaagc aggtaaaata gttgctgaca ctcatgaagt tttaagaaaa gctatttctc
    11881 cgggagtatc tactttagaa ttagataaaa tagctgaaga gaatataaga aaatacaatg
    11941 cagaaccttc ttttaaagga tatggaggtt ttccaggaag tatatgtgct tctatcaata
    12001 gagaggtagt acatggtatt ccaggagaaa ctatattaca agaaggtgac attgtaagtt
    12061 tggatatagg agcctattat aaaggatatc atggtgattc tgcgaaaact cacggagtag
    12121 gcatgatatc tgaagaagat aggaaattga tagaagtaac tagagaaagc ttctatgaag
    12181 gaataaaatt tgctaaatta ggatatagac tttcagatat ctcacacgca gtacaaacac
    12241 acgttgagaa acatggtttc tcggtagtta gagatttagt gggtcatgga gttggagcaa
    12301 atcttcatga agacccacaa gtacctaatt atggactccc aggaaaagga ccaagactta
    12361 gagaaggaat ggttattgct atagagccaa tggttaatgc aggtcgttat catgtaaaga
    12421 ctctatcaga tgggtggacg actgttacaa tagatggaaa aaaatcagct cattatgagc
    12481 atacgatagc aattactgaa catgaacctt tgatattgac aaagttgtaa tttaaaaagt
    12541 aggtgaaaaa agtgctatca gataatttaa gtataggtca agttgttaaa gtttcattag
    12601 gacgagataa aggaaattta ttctttgtag tcaaaataat taataatgaa tatgtattaa
    12661 tagctgatgg aaaaaagaga aaacttgaca aacctaagtt aaagaaagtg aagcacttaa
    12721 aaaaatataa ttttattaat gatgaagtta gaaaaagagt agtatctgga caagagatta
    12781 cagattcttt tttaagagct gaacttacta agttaaatta gataatttaa cttattgaaa
    12841 tggagggctg gttagttaat ggccaaaaaa gatgttatag aattagaagg tacagtttca
    12901 gaagctttac ctaatgctat gttcaaagtt aaactagaaa atggacatga gatattatgc
    12961 cacatttctg gaaagctaag aatgaacttc ataagaattc ttgaaggtga caaggtgaat
    13021 gttgaacttt ctccatatga ccttacaaga ggaagaatta cttggcgtaa gaagtagact
    13081 atattaatat ttagtctaag gagggattaa taatgaaggt tagaccatca gtaaaaccaa
    13141 tatgtgaaaa atgtaaagta ataaaaagaa aaggaaaagt aatggttatc tgtgaaaatc
    13201 caaagcataa gcaaaagcaa ggataagaaa ttattactag cattttgtta aattttatag
    13261 tataatacta tattgtgatg ttaacaaaag aacgttaaaa tcgttgaaca atctggcgta
    13321 ggattggcat atgatgtgaa gttttaaaca ctaaaaacta tgtgtaggag gtgcgatcat
    13381 ggcaagaata gctggggtag atttacctag agaaaaaaga gcggagatag gcttaactta
    13441 tatatatggt ataggtaaag caactgctaa cgaaatatta gctaaggctg agataaatcc
    13501 agatactaga ataaaagatt tatcagaaga tcaagttaat gagttaagaa aagttataga
    13561 tgacgatttc ttagttgaag gtgacttaag aagagaaatt gctttaaaca taaagagatt
    13621 aagagatata aaatgttata gaggaataag acacgctaaa ggtcttccat taagaggaca
    13681 aagaactaag actaatgcta gaactagaaa aggtcctaga aaaacagtat ctcgtaaaaa
    13741 gaaaaaataa ataaataata actagatgaa ggagggaaaa ggaaaatggc taaaccaaaa
    13801 aagaaagtta cacgtattag aagaagagaa cgtaaaaata tagaacgtgg tcatgctcat
    13861 atacaatcaa ctttcaataa tacaataata actttaactg acgttcacgg aaatgctata
    13921 tcttgggcaa gttctggaca attaggattc aaaggatcaa gaaaatcaac tccatttgca
    13981 tctcaaatgg ctgctgaaac agctgcaaaa gctgcgatgg aacacggact aaaaagtgtt
    14041 gaggtattcg taaaggggcc aggttcagga agagaagctg caataagagc tttacaagca
    14101 actggactag aagtaactat gataaaagac gttactccaa tcccacataa cggatgcaga
    14161 ccaccaaaga gaagaagagt gtaattaata agagtacaat aggaggtgta gataaatggc
    14221 aagatataca ggtgcatcat gtagacaatg ccgtagagag ggaatgaaat tattccttaa
    14281 aggtgataga tgttatacag acaaatgtgc tatagtaaaa agaaactatg ctccaggtca
    14341 acatggccaa ggaagaaaga aagtttctaa ctatggatta caattaagag aaaaacaaaa
    14401 agttaaaaga atatatggag ttttagagac tcaatttaga aatttatatg aacgtgctga
    14461 aaatatgcct ggtaaagcag gtgagaactt attaagttta ttagagagaa gattagataa
    14521 tgtagtttac agaatgggac tagcatcttc tagaaaagaa gctagacaat tagtgactca
    14581 tggtcatttc actttaaatg gaaataaagt agacatacct tcattaatag ttaaggttgg
    14641 agatgttata gaagttaaag agaaatcaag atcttctgca aaattcaaaa atttagtaga
    14701 agttaactca agaatagctc ctaagtggtt agaagctaat gtagaaggaa tgacagcaaa
    14761 agtagttggt gttccaacaa gagaagatat agatcttgag atagcagagc acttaatcat
    14821 agaactttac tctaaataat aagcacactt ttaaaaatta ttttaaaatt ttgtttaccc
    14881 tcagtggatt actgaatttt aaggagggtt ttgtccatga tagaaataga aaagccaaaa
    14941 gtagatatag ttgagcttag cgaagactat agatatggta agtttgttat agagcctcta
    15001 gaaagaggat atggaataac tataggtaac gcattaagaa gaatattatt atcatcatta
    15061 ccaggtgtag cagtgaatgc tataaaaata gatggagttc ttcatgaatt ttcaacaata
    15121 cctggtgtta aagaagatgt tactgagata atattaactt taaaagagct ttcagcaact
    15181 atagatggtg aaggaagtag aacacttaaa atagaagctc aaggaccatg ctctatcaca
    15241 ggtgcagata taatctgtcc tccagatgtt gaaatattaa gtaaagattt agcaatagct
    15301 acattagatg ataatgctaa acttaatatg gagatatttg tagataaagg tagaggttat
    15361 gtttctgctg aagaaaataa aacagagaat gttccaatag gtgttttacc tgtggattca
    15421 atatatactc ctgttgagaa ggttagttat catgtggaaa acacaagagt tggtcaaaaa
    15481 acagattatg ataaattagt actagaagtt tggactaatg gtagtataaa tcctcaagag
    15541 ggaatatcat tagctgcaaa ggtattagtt gagcatttaa acttatttat agacttaact
    15601 gaacatgtaa gtagtgttga aatcatggta gaaaaagaag aagatcaaaa agaaaaagtt
    15661 cttgaaatga ctatagaaga attagattta tcagttagat catataactg tttaaagaga
    15721 gcgggaatta atacagttga agaattagct aataaatctg aggatgatat gatgaaggtt
    15781 agaaacctag gtaagaagtc attagaagag gtaatacaaa aactagaaga acttggattg
    15841 ggattaaaac caagtgaaga atagcgagca aaggagggat aagaatggct aagtaccgta
    15901 aattaggacg tgaaacagct cacagaaacc ttatgttaag aaacttagta acttgcttac
    15961 taagaagtgg aagaatagaa actacagtga ctagagcgaa agaaactcgt agaatggctg
    16021 aaaagatgat aactcttgct aagagaggag atcttcatgc tagaagacaa gttttagctt
    16081 atgttatgga tgaaacagta gttaataact tattcacaga tttagctcca aaatatgctg
    16141 agagaaatgg tggatacact agaataataa aaatagggcc aagaaaaggc gatgctgctg
    16201 aaatggcttt tatagaatta gtatagtaag gaaaggatta ggcttgatgg cttaatcttt
    16261 tttttatttt tttgactaag gtatgatata ataatgtgta aaatgatttt tataagttat
    16321 tgcttactaa aaggagtatg agttaatgga taatatagta aaggtaaata atatttcgtt
    16381 tgagtatatt acagatgaag caaaacttaa agcaatagat aatttaagcc tagatgttaa
    16441 aaaaggagaa tttgttgcga taataggaca taatggttct ggtaagtcta ctttatctaa
    16501 aaacttaaat gctattctta tgcctactga aggaaatatt cttattgatg atatggatac
    16561 taaagaagaa gaaagattat gggatataag acaaactgca ggcatggtat ttcaaaatcc
    16621 agataatcaa atagttgcta ctatagtaga agaagacgtt gcatttggac ctgagaactt
    16681 aggtatagaa ccaaaagaaa taagacgaat tgtagaggag tctttaaaaa gtgtaggtat
    16741 gtatgattta agagatagac agcctcattt gttatcagga ggtcaaaagc aaagagttgc
    16801 aatagcagga attattgcta tgaggcctaa atgtataata tttgatgaag caacagcaat
    16861 gttagatcca tctggaagaa aagaagttat gaaaacaata aaaagactta ataaggaaga
    16921 aaatataact gttatacata taacgcactt tatggaagaa gcagtagaag ctgatagagt
    16981 tgtagttatg gaaaaaggaa agaaaatctt agagggaact cctagagaag tattcagtaa
    17041 aataaagatg ttaaaggaaa tcggtttaga tgtaccttgt atgacagagt tatctagttt
    17101 attaatagaa gaaggtataa atattagtag tgatatatta actgtggatg agatggtgat
    17161 ggaattatgt caattatagt aaagaattta acacatatat ataatgaagg gatgcctttt
    17221 gcaagtaagg cacttgatga tgtttctttt gagattaaag atagagattt tgttggtctt
    17281 ataggtcata caggctcagg aaaatctaca ttaatacaac atttaaatgg attactaaag
    17341 ccttcctcag gagaaatatt tataaatgat tttaatataa cagataaaaa cttgaattta
    17401 actgagatta gaaaaagagt aggggttgta tttcaatatc cagaatacca attgtttgaa
    17461 gaaacgattg ataaagatat agcttttgga ccctctaatt taggcttaga ggagtctgag
    17521 atacataaca gggtaaaagc atcaatggaa gctgtaggac ttgattatga gggatttaaa
    17581 gacaaatctc cttttgaatt atctggggga caaaagcgta gagtagcaat tgctggagtt
    17641 atagccatga acccagaagt tctcatattg gatgaaccaa ctgctggtct tgaccctggt
    17701 ggaagagatg aaatttttaa cttaataaaa gacttacatg aaaaaaagaa tatgactata
    17761 atactatcat cacatagtat ggatgatatg gcaaagttag caaaaacttt aattgttatg
    17821 aatcatggta gcgtagaatt tatgggaact cctagagaag tatttaaatc aaatgctagt
    17881 aaattaaaag atataggatt agatattcca caagttttag agcttgcatt gaaattgaga
    17941 gaaaaaggct ttgacatcag tgaggatata ttgactttag aagaagcaaa acaggaaata
    18001 ttaaaagttg tgagaggacg aggattatgt taaaagatat aactataggg caatactacc
    18061 caacaagttc tgctattcat aaattagatc caaggataaa gcttgtagcg acaattgtat
    18121 ttatggtatc tatatttgta gtaaataaat tttggcctta tatagtagtt ttattatgtt
    18181 tattagctat gataaaattg gctaatatac cagtcaaata tattgttaag ggtgttaaac
    18241 ctcttaaatg gattattttg tttacatttc ttataaatat ttttttctta ccaggtgatg
    18301 aaatatggtc atttggattt ttagctatta caaaacaggg tctaagacaa gcaattttta
    18361 tggcaataag attaatattt ttagtagttg gtacatcatt gcttacttta acaacttcac
    18421 caatagaatt aactgatgga atagagagat tgttgaatcc atttaagaaa attggtttgc
    18481 cagttcatga acttgcaatg atgatgacaa ttgctttacg ttttattcct acattgttag
    18541 atgagacaga caaaataatg aaggctcaga tgtctagagg tgcagatttt gaaagtaaga
    18601 atcttataaa tagagcaaag aatttagtac cacttttggt acctttattt gtaagtgcat
    18661 ttagaagagc tgatgaattg gctatggcca tggaggccag atgttacaga ggtggacata
    18721 ataggactaa gatgagagag tctgttatat caagaaggga ttatatggca tgtgtatttc
    18781 aggttgttta tctgggagca attatagcta ctagatttat agcaatttaa ttaataagtt
    18841 atagatataa tgaggtaaag tatgagaaat ataaaaatta aaatccaata caatggtaag
    18901 aattattgtg gatggcaaaa gcaacctgat tctttaggga tacaaggtac aattgaaagg
    18961 gctatatatg atataacaaa agaagaaacc agtcttatag ggtctggtag aactgattca
    19021 ggagttcatg ctattggtca gattgcaaat tttaaaatta attcaggtat aagtatagaa
    19081 agtataccaa tggcactaaa tgccaagttg ccaaaagaca tatcagttat agaagcatgt
    19141 gaagtaaatg atgattttca ttccagatac agtgcaaaag gtaaaacata taaatattta
    19201 gtatataata gcaagtttag aaatccaata cttagtgaga tatcatatca agtaaaatat
    19261 gaacttgatt ttgataaaat gtgttcagaa gcaaaaagtt tattaggaac acatgatttt
    19321 aaaggtttta tgagttctgg ttcatctgta aaagatacag ttagaacgat atatgatatt
    19381 gatataagta aaaaagatga cttgattact tttgagatta gtggtaatgg ttttttatat
    19441 aacatggtga ggattattgt tggaacactt gttgatatgg gtcgtggcag aattaacgaa
    19501 ccatttttag atataataca atcaaaaact aggtcaagat gtggacatac agctccagca
    19561 caaggattat ttctcaaaaa agttcattat taacttgaca caccaggcta catgtattaa
    19621 aatatatgag aatgtgttaa taagtccact tgccccggac ttgacataaa cggtaaaaaa
    19681 attttatgaa aagtagttaa ggagggacag ttatgaaaag ttatatagct aagccagctg
    19741 atgtacaaag aaaatggtac ttagttgatg ctgaaggaaa aacattaggt cgtttagcaa
    19801 cagaaattgc gacagtattg agaggtaaac ataaaccaac atttactcct cacgttgatg
    19861 gtggagattt tgttgttgta gtaaatgcag aaaaaatagt tttaagtggt aagaaattag
    19921 atcaaaaata ttacagatac catactggtt atgtaggtgg attaaaagaa atatcttaca
    19981 gagaaatgat ggataagaaa ccagaagaag ttatatcaca tgctgtaagc ggtatgttac
    20041 ctaaaaataa attaagaagc agaatgatga ctagattaag agtatttgca ggtgctgagc
    20101 acactcatgc agctcaaaat ccagaagttt taaactttaa ataagattaa taggtgaaag
    20161 gaggagttta taatggctaa cgttcaatat tatggaactg gaagaagaaa aagttctgtt
    20221 gctagagtaa gactagttgc aggtgaagga aatatattag taaatggaag agcattagaa
    20281 aattatttta attatgagac attaataaga gatgttaaac aaccattagt tttaactggt
    20341 aatgaaaata aatatgatgt tatagtaaaa gttgaaggtg gaggattcac tggacaagct
    20401 ggagctataa gacatggaat atctagagct ttattaaaag ctgatttaga tttaagacct
    20461 gctttaaaga aagaaggttt cttaactaga gatgcaagaa tgaaggaaag aaagaaatac
    20521 ggattaaaag cagcaagaag agctccacaa ttctcaaaaa gataattact tatttttttg
    20581 gaaaagcaaa atcctttatt tactgttatt cagaaagtat tggtatttta aaaatgttgg
    20641 gttaagttaa ttattgatat ataataaaat aataagatat cttattagga tgtttttcat
    20701 taagaagaac atccttttta ttttgactat ttaataaaaa catatgctaa taacatttaa
    20761 attttatttt tataatattg taatttaaaa gtttatataa ggttatatat tttaagatat
    20821 tatttaacaa tataaattct gaatatacgt tatcaaagtt tataatataa actaagtata
    20881 atttattgta tagtattgaa tgttagataa acatagtgaa ttaaataggg gcagaaatat
    20941 aatattatta cataataata tattaatacc aataaataaa gatgtatcaa tattaaaaaa
    21001 atttaaatgt ttgtactaat atatggaata ttatagtgtt tttataagta tacatataca
    21061 gggtgatgtt gtgagaaagt acataaaaca tataattttt agttttgcaa tgatatgtct
    21121 agtagtagta tccatatttg aaattaaaaa tatttctgaa gatgttatca agtatatgcc
    21181 agtaacaaac aaaactataa ttttagatgc tggtcatggg ggcattgatc caggtgcatt
    21241 aaataaggat aagagtacat ctgaaaagga tattaaccta gcaataacac ttaagcttag
    21301 agagcttata gaatcaagtg gtggccttgt aatattaact cgagaagatg acagtagcct
    21361 ttataaagaa gaaaataata aaactacaag acaaaaatat aatgagaatt tgaaaaatag
    21421 aaaagaaata atatctaatt ctaatgccaa tatgtttgtc tctatacatc ttaatgcatt
    21481 tgaacaatca aaatactatg gagctcaaac tttttatcct aaagacaaac aagacagtaa
    21541 agaattatcg aagtgtattc aagaagaact taaaagagta gtagataaga caaacaacag
    21601 agaagtcaaa ccaagggatg atatatatct tttgaaagaa aataatattc catcagtact
    21661 gatagaatgt ggttttttat caaatgaaaa agagtgtaaa ctcttaactg atgaaacata
    21721 tcaagaaaaa atagcatggg caatctacat aggaatacaa aaatatttaa gttgaaataa
    21781 ctataaaaat aggtctaaat atgtaaatag cttgtattaa agcagttaaa atgcattaat
    21841 ataggctatt tttattttga caaaaaaata tttaaaataa aagttaaaaa gttgttgact
    21901 tagaataata tagatgatat tatatatgag tgcccaaaag gagcaccaaa ataagacaaa
    21961 agaactttga aaattaaaca gtaggttaat tta
//
LOCUS       DAEORZ010000056        21551 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEORZ010000056 DAEORZ010000000
VERSION     DAEORZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.056"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..4470)
                     /locus_tag="KRH39_001956"
     CDS             complement(<1..4470)
                     /locus_tag="KRH39_001956"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511859.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGTLKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKSIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVIFSPYCTMVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDVFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSTYVNGLSNALISQYT
                     EIGVSNVTDAYSAYNATFIVADEKKVYATGINTNYLLGTGNGDGSGVNLSLLNTWYYI
                     NVSGESYNRISCINDITKINNIIIYEYMAIFCTNFGSFITGYHGTLWTKPSGSNYKVQ
                     HQGISYAGWVGGDVYNYYPTRCTQSSYSTNFAYLYYGESSSNLKNYNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNISSGPITSDKAILLYKALLYLSSNTLYSFGNISESA
                     KGLDVSDLQGGYNATNYKKVMKNIKNIFIPSYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSISVNRFTDNSKHVFAIKNDNTCYAVGLNNFGQLG
                     IGDNVNRNVFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYICNDVSIIVKNDNTCYVCGFVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNVIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDEYYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNGKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTFT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIITTFTKVLTNVKEIK"
     gene            complement(4487..5470)
                     /locus_tag="KRH39_001957"
     CDS             complement(4487..5470)
                     /locus_tag="KRH39_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG8511860.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKVD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(5471..6169)
                     /locus_tag="KRH39_001958"
     CDS             complement(5471..6169)
                     /locus_tag="KRH39_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG8511861.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGSYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(6166..7218)
                     /locus_tag="KRH39_001959"
     CDS             complement(6166..7218)
                     /locus_tag="KRH39_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG8511862.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTVI
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEINISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(7211..7639)
                     /locus_tag="KRH39_001960"
     CDS             complement(7211..7639)
                     /locus_tag="KRH39_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG8511863.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(7639..7965)
                     /locus_tag="KRH39_001961"
     CDS             complement(7639..7965)
                     /locus_tag="KRH39_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG8511864.1"
                     /translation="MANPINEFIGIIREEGKHHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(7981..9510)
                     /locus_tag="KRH39_001962"
     CDS             complement(7981..9510)
                     /locus_tag="KRH39_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG8511865.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(9792..10214)
                     /locus_tag="KRH39_001963"
     CDS             complement(9792..10214)
                     /locus_tag="KRH39_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511866.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(10214..12613)
                     /locus_tag="KRH39_001964"
                     /pseudo
     CDS             complement(10214..12613)
                     /locus_tag="KRH39_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12613..12780)
                     /locus_tag="KRH39_001965"
     CDS             complement(12613..12780)
                     /locus_tag="KRH39_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511867.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(12807..13253)
                     /locus_tag="KRH39_001966"
     CDS             complement(12807..13253)
                     /locus_tag="KRH39_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG8511868.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(13379..13807)
                     /locus_tag="KRH39_001967"
     CDS             complement(13379..13807)
                     /locus_tag="KRH39_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG8511869.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(13821..14885)
                     /locus_tag="KRH39_001968"
     CDS             complement(13821..14885)
                     /locus_tag="KRH39_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG8511870.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGESDIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(14890..15333)
                     /locus_tag="KRH39_001969"
     CDS             complement(14890..15333)
                     /locus_tag="KRH39_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511871.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTLTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(15582..16022)
                     /locus_tag="KRH39_001970"
     CDS             complement(15582..16022)
                     /locus_tag="KRH39_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511872.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(16127..16324)
                     /locus_tag="KRH39_001971"
     CDS             complement(16127..16324)
                     /locus_tag="KRH39_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8511873.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIEL"
     gene            16493..16813
                     /locus_tag="KRH39_001972"
     CDS             16493..16813
                     /locus_tag="KRH39_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG8511874.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            16895..17401
                     /locus_tag="KRH39_001973"
     CDS             16895..17401
                     /locus_tag="KRH39_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511875.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWGCEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            17565..17981
                     /locus_tag="KRH39_001974"
     CDS             17565..17981
                     /locus_tag="KRH39_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511876.1"
                     /translation="MYKEKLFDNYFKFLALLFWPIMWYKWIVISNGTLENMLFTIYAI
                     IAIVFIILYSVFMIKYKDITQIDFFYRISTLLAFIFTLFSFLIYPKSLFFLYLKIIFT
                     GIYLYYSIVKTLKFKDDEGVVGIMSSLLLIVITLFY"
     gene            18102..18857
                     /locus_tag="KRH39_001975"
     CDS             18102..18857
                     /locus_tag="KRH39_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG8511877.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(19000..19428)
                     /locus_tag="KRH39_001976"
     CDS             complement(19000..19428)
                     /locus_tag="KRH39_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG8511878.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(19849..20049)
                     /locus_tag="KRH39_001977"
     CDS             complement(19849..20049)
                     /locus_tag="KRH39_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG8511879.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(20232..21401)
                     /locus_tag="KRH39_001978"
     CDS             complement(20232..21401)
                     /locus_tag="KRH39_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBG8511880.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSEDIRNYIRQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
BASE COUNT         6825 a         3817 c         2244 g         8665 t
ORIGIN      
        1 cttaatttct ttaacattag ttaatacttt agtaaaagtt gttattattt tatctttttc
       61 actttctcct agtccaaaca atccatttgt attataccct tgagcgtaca actctccctc
      121 attagttaaa acaagtgtca ttgagttata tatacttata tctttaacag aaggtaatcc
      181 agtaaccttt tgtaactctc ttacatcatt attgtgacca actcccaatt ggtactcagt
      241 attctttcca tatgaccata gagtattatc tgtcttaata atataggagt agttatctcc
      301 acaagcaact tttttgacat ctgatatatt tacttcagta aaagttaata attctatatc
      361 ttcaagagtt tgattaattt ctttatatga attacttcct attccatata atttgccatc
      421 cttagcaaca gccaatgaat gtgtttttga tatatcaata tcagatacct caaatggcaa
      481 tattattgaa gtatacttaa aattatcttt aaatatgttt ttataattta tatacttatt
      541 atttgttttt atatacttcc cattaaacat tactagcttt tcatttttct tattaaaaac
      601 tattttatct gatgtgttat ctgttttaaa atatgaatgt agggaacctt caagagcttc
      661 atttttatca ttatcatatc tatggtgaat acctaaatga ttatatctgt tatctcctgt
      721 tgaagaataa aattttccat atatatcttt aataaatata gtattgtaac tagaaaatac
      781 agtatcaaca tctttcttta tagatacttt ataatatgga actgacatat ccctataata
      841 ttcatcagat atgccataag tatctctacc tgatgatatc aaattgttat tattatctat
      901 ataaagtgta ttattagctg ttacaaaaac atctttaaca tcttccacac tatttgtatt
      961 attattattt gctatacttc catatccact aagacctcca acaaatagtt tattgtcatt
     1021 tgttaaaata gctgaagtat atccattaac tactattttt tttgcattta taatattatt
     1081 aattactcgt atccccttta tatcattagt ctctgttgaa aagaataccc tctctttttt
     1141 ccctgtagta taaataacat tatcatttgt tataaaaaat gtagcttcac ttcctgccgt
     1201 cacaacagat tttacattct caatatttat ttttgtaaat gtacttatac ttccttcagt
     1261 aaatccaaaa tatccattta caaatccaca tacataacat gtattatcat ttttaacaat
     1321 tattgataca tcattacata tatacacatc tctaactcca tttataggta tacgtgtaaa
     1381 tatattccta cttgtagtat cacccaatcc taactgtcct tttccattat tacctgctcc
     1441 atataaaagg ccatcattag ttaataatag agatgtgttt ccatatacag ctacatattt
     1501 tacattttca acatttattt tagtaaatac atttctattt acattatctc ctattcctaa
     1561 ctgaccaaaa ttatttaaac caacagcata acaggtatta tcatttttta tcgcaaatac
     1621 atgtttacta ttgtcagtaa atctatttac acttatagat tttatattat caatatttat
     1681 tttagtaaat acctttctat ttatctcatc tcccactcct aattgaccta catcatttaa
     1741 accaacactg taaagcatat tattattagt taataaatat gtagactttg aatggctata
     1801 tatttcttgt atattagaag atatttcact actgctattc tttttattgt aatttatctt
     1861 attatttaaa tttaaactac tattaactga tataccatgc gtacccttag agttatatcc
     1921 acagataaat aagctcttat cagttaatat tgcaaatcta gttttatctc tacttaaatc
     1981 ataagaaggt ataaatatat tttttatatt tttcattacc tttttatagt tagttgcatt
     2041 atatccacct tgtaagtctg atacatctag accttttgca ctttcagata tattcccaaa
     2101 actatatagt gtattagaag atagatataa taatgcttta tataaaagta tggctttgtc
     2161 agaggtaata ggacctgaac ttatattaga tgctgcaata ttattagatg attgattgtt
     2221 aagataattc cttccatatt gatgtatata agatgaacct ccagaaataa gtaaattgtc
     2281 tggattatag ttttttaaat tacttgatga ttccccatag tataaataag caaaatttgt
     2341 agaataagaa gattgtgtac atcttgtagg ataataatta tatacatcgc caccaaccca
     2401 tcctgcatat gaaattcctt gatgctggac tttataattt gaaccacttg gttttgtcca
     2461 taaagtacca tgatatccag ttataaaaga accaaagttt gtacaaaata tggccatata
     2521 ttcatatata ataatattat taattttagt aatatcattt atacaagaaa ttctattata
     2581 actttctcct gatacattta tataatacca agtatttaat aaacttaaat ttacaccact
     2641 cccatcacca ttaccagtac ctaataagta atttgtattt ataccagttg catatacctt
     2701 tttttcatca gccacaataa atgttgcgtt gtatgcacta tatgcatctg ttacattact
     2761 aacaccaatc tcagtatatt gacttattaa tgcattacta agtccattaa cataagttga
     2821 actctttcca gaagtatata tttgcttatt attagttaca attaatgaaa accagtttga
     2881 taaatcagta tttataacaa ctttttttac attatctata gatattttct taaatacatc
     2941 tgaattagaa ggaagaacac ctgctattga gttactattt ggtccagaat aataacaagt
     3001 atcatcattt ttaatgtaga atattcctcc agaaatattt tttgatgcta ctatagactt
     3061 tacattagag atacttaatt ttgtcaattc atttaattga cttggtaagt cagaacccat
     3121 tcctttgtat ctattatttc ctagaccata tacatctcca ttccctaaaa taaccatagt
     3181 acaataagga ctaaaaatga catctcttac agaatgtata tctagtggaa gttcaatatc
     3241 ctttatttcc atttcttctt ttaataaagt taatttatcc tcagatttaa ttaaatttat
     3301 atagttacta taactatatt tttcactatc cattttctta aattctacaa tgtgcatatc
     3361 atcaaaaaac gaacctaatt gtccataagt attatctcct gtacaatatg aatcattatt
     3421 tgaatcaatt agtatactat gtgataaacc aatgaaaata ctcttagcta caatattttt
     3481 taaatctata aatgaattat ctatattctc agagtaattt gctataccca actggtatat
     3541 atcattattt ccagcaacct tcaaagtacc tttgttatct ataagcatag tgttaccatg
     3601 accacatgca atagttttta tatttacttc ttctatctta gtaaatagtg atgattgaag
     3661 tgcatttcca aaacattttt cctttccaca accatatata tcattattat acgtcattaa
     3721 cacagtgtgt tcagaaccac aagctatagt cttaacatca ttaatagaaa ctaaactaaa
     3781 taaatctcta ttattcttat ctcctaaacc tagttgacct tgattattaa aaccagtaga
     3841 ataacattta ttgtcattct ttattataaa agctgattta ttatatgaaa atacagcctt
     3901 tacattgtca acatctactt ttgtaaatgt atttacattt gcagatgctc cattagttcc
     3961 tagacctaat tgaccataca aattatttcc agcaacatat aaagttccat cattgtataa
     4021 aagatatgtt gattgaagtc cacatgcaat atcctttacg ccttttattt ctggaacctt
     4081 ctcaaatgta gaaactgttt tccctacttt ttctaatccc aatcccattt gaccatctgt
     4141 atttatacct gcgacaagta attctccatc tgaagtaagt ataactgcat gagattctcc
     4201 acatgcaact tttttaatat tagtagatat atttggtatt ttcgtaaata attttcttgt
     4261 tgtattatct tctcccaatc caagttgaaa gttactatta tctccacatc catacaagat
     4321 attattattt tttagaccaa atgtatagtt acaacatcca gtaaaaaaat taataaagtc
     4381 ataattatca tacccttcat caaattcatc aactaccata tttttatcgc caaagaaatt
     4441 acctctttga agtagtttag ttcttttcat tataaaaacc tcctaattat cttatttcat
     4501 acgaatattt atttaaagtt atatctgtct gaccaattga atatgcccta ttagattttg
     4561 tttcaacaaa gttattatcc atttcatgta tatatctaac tctgtttgca ttaaatggta
     4621 gcaccacatt cttaaatgta acactaccaa gttctccagt tatacacttt ctatttaaat
     4681 ctaacttaag gccttctata gctattatgt ttttagcatt agttaaggta tcataccaat
     4741 atccagcttc atctacatct atttctgact ctaattctaa gtatattaag atatctaata
     4801 ctttttcaga taatttattg taactatttt ttaatacaga tagttcagtt tgtatttgac
     4861 ctcctattac atcatcaata gatatatttt taattttttc tactacctca tttatagctc
     4921 ctgataagtt tttagcttct gtagataaga gttcttctcc aatttttact tttatttctt
     4981 ttatattttt atcaacctta tcatctaaat cttgaatatc cttttgagtt acaaaaacca
     5041 acgtcgggtc tacttctaat gtaacatttg aagtattgga taactgtaaa atcattttta
     5101 caaccaaatc tttaatactg ccatcttcag cacgtggctt atacgtctct gggtacttac
     5161 ctatagctat tatattattc tcattatcca gaacagcagc ttctcttatc ataaatccac
     5221 caactgttcc tggtaaaata gtctctataa ctatccaatt aggatttttt tcatcaattc
     5281 ttagagaatt tatatttcct tcccaaacta cattctttaa agcagtttgt tcctctgttg
     5341 gctcataata ttctccatta ctatctccaa cttgaaactt ggttaatcca acaagctctc
     5401 ctagcgcagt tgcatttgct attttttctc taccaatttt ggttaatatt atataaaatt
     5461 tttcttccaa ttataccacc tccgatttag gataaactac tacattttct acacctgtat
     5521 catttgcaat agctatatta atcttacaat tattttcaat gcatttaatg cttaaaggat
     5581 aaacctttat ttcttctcta cttaacattc cagctccaca gtttacaaca gcactgcatt
     5641 ttataattgg ttcaaagtat ggatgaactt ttacctcttc actacatacc atacctccac
     5701 cacaatagac catactttta tctagtatta ttggttctaa atagtgtaat aagtgagcag
     5761 gttttactct ttcaatcata tcgctacaat ctaaaagagt tttatagtca cagttatttg
     5821 ctataaaact caatacgaat gtaaattcat cactataaac ttttatatct gtttctgatt
     5881 ttgtatatgc ctcacatata ctttttataa cttcaatagt actagtacct ctacttctca
     5941 ttttagcttt tatattgcta cgtcttgtgt caaaatcaag ttcttttttt tcaatgcaga
     6001 gtatatcttc ccacatatct aatccccatg ttgcagtgtc aacaaataat tggttaaagg
     6061 tatcatcaat atcttcttta agaatattta attctgtatc gtatgaacct tgtatctcct
     6121 ctacaatgta atttctatca aatgatggta gtttatcaat tagcttcatt gattttccac
     6181 ctcactaaaa ttaacagttg ttacactagg tattttatct tcattaatag ttatattatc
     6241 tgtacttcca tttataagta ggttacttaa atcgtgtaca ccagtagtat ttataagtat
     6301 tcccattact tttgtataga ttatctctcc tctaatatct ctaaagtatg tatttatact
     6361 ttctaggaaa gattctttga cattgtctaa tgtatatcca tcttctagtt tcattactgc
     6421 acttatattt atttctattg gtaatggcgt cacaactgtt atagttggcc caataggctt
     6481 ctcttcatct atatgttgct gacacctttc aatcgtttct gtatcaacag cttgattatt
     6541 tttcccaaat atcaagacct taactgttcc cggaccatcc catcttggat aaaccttaac
     6601 attatagact ccatctactt ccaaagccca ttcttcatag tgagctttat ttccacttgt
     6661 agcttgattt ctttggattt tataaaatct ttcctttagc tcttcatctg tctctatatc
     6721 tgtaccacct tcaaaacttc ttgtgttagt aatctttgtt actccagata tattatcaac
     6781 tagtttaaat tcacaatttg taggtaaatt atatttctta ccaacttcca gagcttgaac
     6841 tgggctattg tcaccttctt cactaccaat agttacatct tttattacta caaatagtaa
     6901 gtctctatat gatattactg tgccattatt tattacagtt cctttctctc caataaattc
     6961 aacttctcca tttgactctg tacctaactt tctatataca ccaaattcat tgactctttt
     7021 atcgagaaac tggttatatg tatcttgtat aaaagccatt ttatgtatct ttgaaagttc
     7081 tagatatatc ttcgaaagtt ctagattatt tccagatacc atgttgttta gaaaagaacc
     7141 ttcaccctta taaatatcaa gattaatatt ttcaagagtt ctatttttta ttacttcata
     7201 tgtctggtca ctatacattt atttccacct ccccatagat agtggatact tttacatttg
     7261 cactcaatat gtctctgtta aagttagcac tttttacgtt tacttctaat atatatggat
     7321 ttattagaag ggcctctttt atgaatctac tagcttcact ttctgtaaga ccttttgtat
     7381 atttttgtcc tattagttct gaaagctctg tcccatattc taaagagtat atctcatgtt
     7441 catatctatt tgtctttata cacttgtata tccaaacttt taaggcttct tttttttcaa
     7501 ttattttaaa gtcacctttt tctaaaatag gttcatcttt ttcaaaatcc catgccactt
     7561 cacgaaagat tggcaattct tctgttttag gtaagatata atcctctggg acacctataa
     7621 aaggaaatat tgtactcatt ataagctcac caacttactt acaacagcaa atttttcgcc
     7681 tattctaaac attattacca tatccccaga ttcaaagtta tctataaaag gattttttac
     7741 ttcatgctgg tgttcttgat ttgtttctgt gtcaaatgtt tctagctgtc tatcaatcat
     7801 ccaactatct atcaaaatat cttctttttc taatatgatg ttatttgtct ctatttttaa
     7861 atctggtaat ttacttttaa tttttccaat aaaaaaagaa ggttgattat gatgctttcc
     7921 ttcttctctt attattccta taaattcatt tattggatta gccattatat caccatcctt
     7981 ttataatact ctcctagctg tattaaaatc tcttctacta cttaacttac ttattttaac
     8041 tacatcacct ttttgtggag catgtaaaaa ttctccatta cctatatata atccaacatg
     8101 gctgactgga ttatgaaaaa atactaaatc tcctgcttga agattttttt gttctacttt
     8161 cttgcctttt ttagattgtt gatttgacgt tcttggcaaa ctaacattaa cttttttaaa
     8221 acaatagacc attaaaccag aacagtcaaa actacttggt ccatttccac cccatttata
     8281 aggtttacct agatgttttt ttgcttctga tattactatt ttagctttat cagatacatt
     8341 accagaataa ccaccaataa taatttttcc ttttcttctt ccaaatttac tagcttcttc
     8401 aacatttcca aataaaatat ctatatggta tgttccattc ttttctatag ttattgcagg
     8461 tcctacgtca tttactttat agactccatc acgaccagaa actcctgtaa ttacctgtat
     8521 ttcatctcca tagtttaata aaggatgttt atttaaaaac tcttttgtat aataagtttg
     8581 ctcatattta ccaaccatag gagcagcgca agttttttta gatgggtcaa gttttttctt
     8641 tctacaatct gtatctccac cttcttcttt tctaggacag taagctgtaa attctgctgt
     8701 aaactctttt cctcctgcat aatcttctcc ccctaaatta ctttcttcct taggttcatc
     8761 ctgtcctgct gacttttcat ccataagatt ttgaaaatta agttcaagtt caatttgata
     8821 ttctccattt tgccaagtat gtttgtctgt atctatataa aatagtccta caagctttgt
     8881 ataagaatct ttaactttta ctcctctacc agttatacaa cttacatctc cataaccttt
     8941 aagagaacag cttttttcta tcccattaaa ctcgctatca atatctacat cttgattttc
     9001 ttgttgctga attacttttt gcattattac atttacttcc ttaaaaattt cattatctat
     9061 tttttcgcta atcttgcttc catactggtc taccactatt actttatttt ttacattttc
     9121 catactctcc gaaaaggtgg tatttataat attaaatccc tcttcaaaca taacacttaa
     9181 tgtaacagtt cctttttcaa taacattaaa cttatctaaa ttagcctcta tcatatactt
     9241 tttctttgtc tttttacttg cttctgtata tgcactcatt attgtgtcat aaccatttac
     9301 tcctataaac atctttgtat acttgacatt ggtctttgct attgttccaa ctgaaagcct
     9361 attttcagca aatacttgct ttgctatgtc ttcaactaac ttatctttaa aattgtatga
     9421 cacttcactt tgtgtaagta aaaatcccat atctttagat acaaaactaa tttcattact
     9481 gcttgaatct ttagacctat taattatcat tcctctaaag agttctttct catctacata
     9541 gaaacagact gtactagcta ttggtatatc gatttgttgg aaatttacat caaatgatga
     9601 ttgtattatt gaaaactcta gtgtccttga tggagattta taatcacctg accatgatac
     9661 cttgtctact atgtcagtta tgtcatatat acttccgttt tttatgtgta cccaaatctt
     9721 aataataaaa aatcacctgc cttttcattt aattctatca gcagttattt ataacttatt
     9781 atctataata gttattgttt tggagttact tttggtatct ttattctttt atactctttt
     9841 aaatctaatg taaaatatac atctcgagtc ccatctcttt cttcataact aaacccttca
     9901 attatgactt ccatatttat atttgtttcc gtaattgtaa atcttaatat aaaaccttcc
     9961 ttcatccatt tttctatctt atttacacaa tcatatggtg atggaaaacc tgtatagtca
    10021 cagaaagtgt agtctttatt tggaaaaaaa cttgatattg aaatgctttt aagagctaca
    10081 ccaccaaaag ttgctatttc acctattttg agtatactag aagagtttac agcagctttt
    10141 ccattaatac taaaggaaga tggaaatact ggaaatctaa atctatcttc tgcttgtcta
    10201 agccacattt ccactataat acacctccca attgtgccat ttttatactt gaaactaatt
    10261 ttgatgtaac tttttctata tcagcttctt ctctaattac tattgtatca gctaacttgg
    10321 ctatacttat atttccacca ttttttccac ttctatattg atttgcttct tatttagtta
    10381 aaaccatttc accctcatga agccttgttg ggtaatcatt ataaggaaca tagtttaatc
    10441 caaaggcgtt acttccagat ttgcttgtac ctgcattcca atcatcaatt atagatctaa
    10501 ctgtctcaat aattggattt ttattaattt tttcaactaa attttgccac catttacaaa
    10561 catcttttat aatattgcat atatcattaa atagattaaa aagacctcct aaaactggtt
    10621 ctacaataat ccaagcatcc tctaaaagtg gtcctattgt tgcccaaact gtctcccaga
    10681 taacacctag tgtctctata aagttattta tttgaggggc atattcacct aaaaaagtaa
    10741 aaattcttgt gataatagga ctaacagctt cgactacagg tccgattagt tggaatgctg
    10801 tagttactat tgtaccaaat atacttatta atccttgtgc aattggtgat ttattgttta
    10861 tactatctag taaattattt actaagttta ctactggaag taatgaatct attaatgctg
    10921 taaaatcgat attttgtaat agtgtacttc ctgcgttaac aattgctttt ggtgcattcc
    10981 ctatcattgt tgctgcatga ttttcaaaac gttcttgctt attttcaacc atcattttac
    11041 gcttttcagc ctcattagaa attcgatatt gagtagaatc agaaaactct tttccatttt
    11101 ttacacttct agattgcatc tggtcatatt tgttgccaat aattatctta ccattcatta
    11161 cattgcctat tttaccagcc attctttcag ataatccagc taaattgcct aatccacttt
    11221 ctataaaagt ttttattttc tttagtcctt gacctaaaat atcatcaaaa ggtttaacaa
    11281 tactcttcaa accattgtta atgcttgttt gagctcctga tattatacct ggtattgtat
    11341 tactcatttc tttagctaac cccatctgct ttacacttat accttccaat gcttcatcaa
    11401 aagttttata attttctcct accagagtat taagagattc catgtttcct tttgctgcac
    11461 ttaagaaagc tgctgataca tcttgttcac tacttgtatt catatttaaa gctctaacat
    11521 ctaccatagc ttgagttata tctttttctt ttttgccacc agtaatatta ctaatcattt
    11581 tagctttagt tcccattgca acaacatctt cagggtcaaa aggcgtatca tttgccattc
    11641 ttactaaact gccataataa tcatcaactt ctctttgatt aacttttcca gtatttcttt
    11701 tcattacact ttctagaaca actttctgat tttgttcctg accaagtaaa tcaacaccct
    11761 ttttcacagc gacacctata gtagcaccat ttgtcaaatc agatatcttc ccttttatcg
    11821 aaccaagtat tccacttgcc atatccttta cagctattgc accactccat accttacctg
    11881 caaataatct caatttggga aatactttac ttataatgcc acttgccata tcttttatag
    11941 ctattacacc actccaaaaa gtatttttaa acaacattaa cttaggcaaa attctaccca
    12001 tagcactact caccatatct ttaagattaa tacttccttg ataaggacgt ttctctactt
    12061 gttttaattt tcccaagata ctacttataa ctctcttagt catatctttt gcttttatta
    12121 caatcttcca aaccttgcca ccaatctttt ttaaatttcc aagtaattta tttttagttt
    12181 tttctatttc atcactttta gctttaaata ttaatataat cataatattt gaattaaact
    12241 catttttctt ttgaatcaac ttattaaaat aatcaataat catagctata gttattgcta
    12301 gtctcataat acttccattt aaacttccaa tcactcctga aacagcattt atgctagaac
    12361 ttaattttgt catagttgaa cttacactac tagcagtagt ttgaactttt cttaacgaag
    12421 aatttaaact gtttacccta ctacttgcac ttccaataga attttttatg ctactagcca
    12481 ttacattagt ggaaagtgaa acagccacag ttgctctcct aaaattatca gttactgata
    12541 taatttgatt catttttggt gtgtaattat ctgtaagata catatttata ccttcttctc
    12601 tcacatttcc cattacatac cacctcctgt caaaggacac actccatatc catgtttcat
    12661 tttttcaact tcttcctcaa tttctaaggc gaaaaaagct tcaataagct ttaattcgcc
    12721 tttatccatt gcataaaaaa gggacggtct tatacctttt ttcttccaat aatagtacat
    12781 tattgtagtc ctggtatccg tccctattag ttttttacct cttctatcac tgcattttta
    12841 ccataaccca taacatcttg aagtattcta tataagttgg taatttcacc tggtaataat
    12901 aattttcttg ctaattcttt tggtgttgga gccttaaatt tatccattag ctccttattt
    12961 ttaaataaaa gctttccatc caaatcacaa acaccttcta taattgtaag catctgcatc
    13021 tccggtaagt ctatatccgt tttcatattt tcatcaattt taagacaagt attttgtatt
    13081 tcttcatctt tacttattgt taaagctcta cacattactt taaattcctg cccaaatata
    13141 gttgataatc tcttaagttc aactatagta ctcggtcttt ctaagttttc tgtatctgaa
    13201 tttaaaagta aatctactat gtttatattt tttgataatc cattttcact cattattatt
    13261 tgctccttta tcaatcaaat tcaatcaact aactttttta cttattaact atctgaattt
    13321 attaagtata tatttactaa atatcagtat aaaaccaaat ataaaactaa atataaaact
    13381 aaattatatc aagaaaatca tactcagtaa atgtgaaagg tgcttctatc tctcctttta
    13441 cgccaacctc ccaatcagct aaagttaaat catcaaatgc tatattcttt actgctattc
    13501 tttctgctcc ataagaatct gggtcattta atttagatat agcaacaaat ctaggttcag
    13561 aacctctctt tatcttttct ccaattaact tgtgcattct tgaactaacg tggtatagag
    13621 ttattgagcc ttttccttta tatcccatat actttgtatc agtacccatt tgacctgcta
    13681 ttataatatc ttcttttgta aattccatct ttgcttgaaa tttctttact tctgctactt
    13741 tgtttccatc aagccaaagt tctccccaag taccactcat tacatttcta gcttccatat
    13801 ttgccatatt aataatcctc ctatatttct attgataaat ctatatcttc catagcatca
    13861 agtactttta tttttgcttt taaaaatact ttagaacctg tgttagcttc ttttatttct
    13921 tgtaatgtca tataagataa atctactcct tttgatttta aatacgattt ttgtgcttca
    13981 aaatctattt caacagtaga atcagattct ataagtgctg atttttctaa ttcttctaaa
    14041 taacttttta tagctactat caataaacat ttgttgtcat aactgttagt aacctttcct
    14101 atatagtcat ctattatcac ctttcttatg tcactatgta taatatctaa agtgtcaact
    14161 atttttattt tctggaacat ttctcctttt tctgctgtta attcagttaa agaatttact
    14221 cctctagcaa ttcttatagc tcctgcttcc ttaataagta taagctctcc tttattaacc
    14281 cttgattctg catcaacttt agtcatctta ggtatatcga ctacatcact aagcttagta
    14341 taagttactg attgacttaa aggtgtacct gctataagcc cagccaccct acttgtaaac
    14401 tcatcaacac tgtatttctt ttctccaact aacacatctt ctgtagtaaa attaattatc
    14461 ccttcatgat ttcctacaac ttttcctaat acagctttaa ccttaacctt atctatatct
    14521 ctaagtttaa ttatccaatt ttttatagca gtcttatcag cttctactgc ttttggcata
    14581 catagataat taaattcctt agtctctaaa aaatctaatg cagtttgaat atctgattct
    14641 ccttctatta cataaactaa taacttattt ggagtgttaa catttcccat caaagctaaa
    14701 tttatatatt ctttattttc agcagataaa tcaactggta tatcctcttt ttcatgtatt
    14761 tcattaagac ctagtgcctt agcatcttta agcaccattg caattattcc tctagctgaa
    14821 cgttctttaa cagttgtagc tagctcctta aatgatatgt tgatacttgg taatcctata
    14881 gccatactat cactctccta aatttaaatt gatttcttcc atattttcat atactacatc
    14941 ttcttcatat acactatcca gatagtttag tgtaagtgta aagttcaata cataaattga
    15001 ctcttctttt ttaatacttt gttctagctt tgttatattt aattttctgt ctgttacctt
    15061 tatatttctg ttaaatagct tatttaattc atctgaaact tcatacattc taatactctt
    15121 tgactgtggt tttggtaagt attttataga gataacaata tctttattat tcgtatttaa
    15181 cattataggt gtacaaataa ctgatagtat acttacaaaa aaacaagacc cttcagagcc
    15241 ttgcacactt tcatcatcaa taaaaatact ttcagtaaag ttttttttga gaatctcaat
    15301 aattgtttct aatatttcct tgtatttaag caaataatca ccacactatc actaaatatt
    15361 cttgcttaat ctgataatct tactataaat gatataagtc atttttaaat atatatctca
    15421 tttattcctg tcaagtatct tctatatttt taggtaatat tattcttttc aatctatcac
    15481 ctccttcaat atggatttct ttcacaataa cattatctca catattttat tgcctattgt
    15541 tgcaatggtg tatataaagt gttgtaaaat tgtcctattt tttataatat ctcactcagt
    15601 ttaaatatca tctttttctt taaattgtaa caagatgttc tatctaagtg tagtttcata
    15661 gaaatatatg tcatattgtt ttttgtctta ctattataaa aaagattaaa aaattccatt
    15721 tctatatcat ttagacatgt tagagcattt tctatcttct ctttttctat ttctatatct
    15781 tctttttcca tttttaattt tcttaatctt tcctctcttt ttaatacctc attttccaca
    15841 gaagaactta tgttatatgt gttactagtc ttttctgtat ataccattgc tccacatcca
    15901 gagtattcat tctcacatct ttttatgtcc aagttaatat tctttatctt tatatccaaa
    15961 tacttataat tatgtaatct tttatctgtt tctttaaata atatatcttt tttgttttcc
    16021 atagcacttc tcctttttta tataattatt attagcttca aatttacaat aatctcttta
    16081 gtgcacaatt ttttactaat ctcacaactt tcaagaacat attgttctat aactctataa
    16141 aaaaaacttc ttctatattt tgttctaaaa tactagaaat cttcttagcc ataagaagtc
    16201 caggattttt tatacctctt tctatttgag atatatagtc atttgtaatt ccagcctttc
    16261 tggcaagttg aatttgtgtc atacctttgc attttctata atatttcaag ttatttttaa
    16321 acattgtttt atacctccat tactattaag aatattttgt tcttccatat ttagattata
    16381 tagaattatt tgttctttat caatagattt tagaaaaaaa tattctttta tattttatta
    16441 gttttatttt ttagaacaat ttgttataat ctatacataa ggtggtgtta taatgtttgc
    16501 caaaagatta agagaactta gaaaggaatt tggattgact caaagagaac ttggagaaaa
    16561 agtaggcgtt tcccaaagag tcttagggta ctatgaaaca gaaaacagat ttcctgatga
    16621 acacatttta aataaattgg ctgatgtatt taatgtatca gttgattacc ttcttggaag
    16681 aaccttagtt aaggaaaata ttgatacagt agctgcacat agaaaaaatc cacatgaaga
    16741 gttacctgaa gaagcacaag aacaacttaa tgattatata gaatttttac taaataaata
    16801 taaaaaaaaa taaaccatat gatgagcagt ctatttttct gctcttttaa tataacttaa
    16861 aggcaaacat acattctatt ttagggggat ttttatgaat aatttggata agctttttga
    16921 attagcttct caagaagaga taataattca ttataccact tatattgcag gtgatttaga
    16981 aggactatat ataaacaaac atggtattaa aattatatca ttactcagta atttaaaaca
    17041 aaactcaaaa aaattgacat ccatcttagc tgaagaactt gggcatcatt tcactagcct
    17101 tggatactat gtatcctctt ataatgatta ttatacaaaa attattatag ataaatgcga
    17161 aaataaagcc ttaaaatggg gttgtgagtt cttaattact gaagaagata taataaacat
    17221 aattaattct ggcatcactt gcgtttatga aatggcggac atacttaatg tggatattac
    17281 tttctttcaa aaaagattag aatttttatc attaaaaaaa cagtctttac aacttggaaa
    17341 taataaatat ttaatattaa ctaatttgcc gtatttctac atatttgatc caatttcata
    17401 ataaaaatag tagtaatttt aactggctta acagtaaaat ctatatattt attttaacaa
    17461 attttggttt ctataaattc aatattaata tataataatg atatcatatt atttaaaatt
    17521 ctaaaaacaa atatatgctt ttgttaagaa aggttgtttg atgaatgtat aaagaaaagt
    17581 tgtttgataa ttattttaag ttcttagcac tattattttg gccaattatg tggtataagt
    17641 ggatagtaat ttcaaatggt actcttgaaa atatgctctt taccatatat gcaatcatcg
    17701 caattgtttt catcatttta tattcagtgt ttatgataaa atataaagac attacacaaa
    17761 tagatttctt ttacaggata agtactctac ttgcttttat atttacactt tttagctttt
    17821 taatataccc aaaaagttta tttttcttgt atttaaagat tatttttact ggaatatacc
    17881 tttattattc aatagtaaaa acccttaaat ttaaagatga tgaaggtgta gtgggtatta
    17941 tgagctcact tttattaatt gtaataactt tgttctacta aatttaattc tatttgactt
    18001 aatatggctt taaatattag acatctacat tattttctga tattatttaa gatggtagtc
    18061 ttatattaat aatgaaatac actatgaagg agttaactat tatggaaaaa aaagttttag
    18121 caacagttgg agaaaaagag ataactaata ttgatattga aaatgcatta aaatcattag
    18181 acccttatca agctatgcaa tttaaaactg aagaaggaaa aaaacactta ttaaatgatt
    18241 tagtgaatca agaattattc ttcttagatg ctaaagaaga aaaattagat gaagaagaaa
    18301 tatttaaatt agaaatgaaa aagatagaag aaaatgtatt aaagcaattt gctataaata
    18361 aagttttatc tagtgttaat gtaacagaag atgaaaaagt aaaattcttt gaagctaata
    18421 agagctcttt ctctaaacca gaaagtgcaa ctgctaaaca tatattagta gatagtgatg
    18481 aaaaagctaa agaaatactt gctcaaataa aatcagaaga aatatctttt gaagatgcag
    18541 ctcttaaaca ttcttcttgc ccatctaaag atatgggtgg tgatttagga acattcggta
    18601 gaggacaaat ggttcctgaa tttgaagaag ctgtgttctc tatggctaaa ggtgaagtaa
    18661 gtgaaccagt taaaactcaa tttggatacc atataataaa attagaagac cttcaagaaa
    18721 gtacagagtc tacatttgat gaagttaagg ctgaagttga aaaaagttta ttatatcaaa
    18781 aacaaaatga agtttatgga aataagataa atgctcttaa tgctaagtac ggaaaccttg
    18841 taaaatacaa cgattaatca taaggtcata tataaaaaag ctgtcttttc acaatattta
    18901 ttagaaaaga cagcttttta tattaaagtt taaatattca gtttcatatt atcattcttt
    18961 tattgtaaag aactcagctt aaaagtttaa ataagatttt taattatcat tagctaaatt
    19021 tttatatcca cgtattatct cataacagta acctattaca tctcttcttt ttattatccc
    19081 aataaatgtg tcttggtcat ctataacagg aatgaaattt tgatttaaag attttataac
    19141 taaatcttca atatctgcgt tgatggaaac aggagaatta tccatttttc tatttatatc
    19201 tgtcactgga acatcttcca atccctttaa gtccagattt aaatcatttt ttaatgtcca
    19261 taaaaaatca ccttctgtta tagttccaac atatttacca tccttactta ttattggaat
    19321 tgcagagtat ttgtgatatt ccattttttc tagggcttgc cttatagtgt agtcttcata
    19381 tatatatgct acttcacttt ttggtgttag aaagaataat atgttcatta cactacccac
    19441 cgttactgaa aacttataaa tattactcaa tatttataat gttacttttt tgcagtaact
    19501 ttctcctttc ttattttcta agtattctta atactatacg ctgtaacaag cgtttttcct
    19561 ttgctcattt taatcttatt atgctttatt aatattttta tatggtctat cagtatcact
    19621 tattctcttt ttagaaaatt tactaaaaat taattgactt aaacttagcc ataataattt
    19681 tactctacac tattaatttt aacatattta tctcattatt aatgatttta ttttaataat
    19741 tttaatttat tttgctatta attttattta caaaaactga aaaaaccgct aaatgattag
    19801 cggcttatct ctcatattac tagaacacta atatatatga atttttaatt ataatctaac
    19861 tacgttagca gcttgaggac ctttagctcc atctactact tcgaactcaa cgctttgtcc
    19921 ttcttctaat gttttgtatc catctccagt tatagctgag aaatgtacga aaacatctcc
    19981 ttcaccttct acagatatga atccaaatcc tttttcgttg ttaaaccatt taactacacc
    20041 agttttcatt aataaatcct cctaaaaaaa cacaaatatt tagctactct tttctgtaac
    20101 cttattacta ctctatcata tatagctatg tttgtcaatt attattattt ttatacatta
    20161 ataatcaatt tttttacttt ataattttaa atgattatta tcttttacac tttatttttc
    20221 tttataattt tttaataatt tatttttatt atatggaatc ttatggactt atcactccaa
    20281 cctaaatccc atggcaccaa cattctatca gtattatgac aagttaccaa aggataccct
    20341 cttgagtcta atcctgtcac tgtcgaaaca tgtgttattc tgcctttctt ctcataacca
    20401 acaaaatccc ctggacgtaa attataagct tctttgtata cttcttcata ggaaccttta
    20461 gcaattaatg aacctcttcc actatatagt gcataatttt taaaagcttg tgcatttacc
    20521 catgcttttg tacctgctct ctcgctataa ttccaaattg agttcttttt gaaccttcca
    20581 ctttcaaaca ttatctgtga tgcaaagttt gcacaatctc caccttctgg attataatcc
    20641 ctatagtttg gattaaattt cataccatgt tcttcatcag ctgcggctcc acagtattta
    20701 tgtgcataat ctatagcctt tttttgttca tctgtaagct gtaactctac accatcttgt
    20761 tgcctaatat agtttcttat atcctcagat tttatatttt ctaaatttaa agagtctgca
    20821 aaagggtctg tgtaccactc tttagttatg acatattgat tatctataat ttttacattt
    20881 agataatgat atgtacctat tctaaaggca ttttctttgt ccttttgatt ttcatatgaa
    20941 tacttatatt cagtcgatac tgaacagatt ataccataga ggtctttttc tttttctttt
    21001 atttttttaa tcttaaccct tgtctttatg tctttaaatt ttacaccttg cttactagac
    21061 cagttttcta aatatttcat cttttttact tcattttcat aagcccacag accaaatttt
    21121 ttatccgtct cataaagacc ttttaaaata ttttcatttt tctctaatat tgccttattt
    21181 ctgtagtcaa ataagttttc aagcaatagt ttatattggt cttttacatc atcaccttca
    21241 acatttgcag attccatgac tttttcttcc atatttttca tacttaaatc attgattaaa
    21301 aatacacctg ctgtagtagc taaagacaaa aatacaaatg ccattatacc tttgatgtac
    21361 ttattcttat ttttcagtat tttcaaagct acacctccca aatagtacac cttacaaatc
    21421 aaattacaaa gaatattctg tcacatatag taattttcat atttaataat tctctatttt
    21481 attatgtcca atattaaaaa ttaattgtat caatacactg ttttttttac aattttttat
    21541 tttataatct a
//
LOCUS       DAEORZ010000057        21459 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEORZ010000057 DAEORZ010000000
VERSION     DAEORZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21459
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.057"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            394..915
                     /locus_tag="KRH39_001979"
     CDS             394..915
                     /locus_tag="KRH39_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8511881.1"
                     /translation="MDIAKLILNPARLRILQYIRLHSSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            949..1725
                     /locus_tag="KRH39_001980"
     CDS             949..1725
                     /locus_tag="KRH39_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8511882.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            2068..3021
                     /locus_tag="KRH39_001981"
     CDS             2068..3021
                     /locus_tag="KRH39_001981"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBG8511883.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            3039..4058
                     /locus_tag="KRH39_001982"
     CDS             3039..4058
                     /locus_tag="KRH39_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBG8511884.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSRENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            4089..4340
                     /locus_tag="KRH39_001983"
     CDS             4089..4340
                     /locus_tag="KRH39_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511885.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4622..6427
                     /gene="lepA"
                     /locus_tag="KRH39_001984"
     CDS             4622..6427
                     /gene="lepA"
                     /locus_tag="KRH39_001984"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBG8511886.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6576..7913
                     /locus_tag="KRH39_001985"
     CDS             6576..7913
                     /locus_tag="KRH39_001985"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBG8511887.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVGLFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            8146..9459
                     /locus_tag="KRH39_001986"
     CDS             8146..9459
                     /locus_tag="KRH39_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG8511888.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9631..10806
                     /locus_tag="KRH39_001987"
     CDS             9631..10806
                     /locus_tag="KRH39_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBG8511889.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10909..11928
                     /gene="hrcA"
                     /locus_tag="KRH39_001988"
     CDS             10909..11928
                     /gene="hrcA"
                     /locus_tag="KRH39_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBG8511890.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11958..12578
                     /gene="grpE"
                     /locus_tag="KRH39_001989"
     CDS             11958..12578
                     /gene="grpE"
                     /locus_tag="KRH39_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBG8511891.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12670..14517
                     /gene="dnaK"
                     /locus_tag="KRH39_001990"
     CDS             12670..14517
                     /gene="dnaK"
                     /locus_tag="KRH39_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBG8511892.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14728..15882
                     /gene="dnaJ"
                     /locus_tag="KRH39_001991"
     CDS             14728..15882
                     /gene="dnaJ"
                     /locus_tag="KRH39_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBG8511893.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEIPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16848..17783
                     /locus_tag="KRH39_001992"
     CDS             16848..17783
                     /locus_tag="KRH39_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG8511894.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EGLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17819..19192
                     /locus_tag="KRH39_001993"
     CDS             17819..19192
                     /locus_tag="KRH39_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8511895.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19380..20147)
                     /locus_tag="KRH39_001994"
     CDS             complement(19380..20147)
                     /locus_tag="KRH39_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8511896.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(20177..21286)
                     /gene="ugpC"
                     /locus_tag="KRH39_001995"
     CDS             complement(20177..21286)
                     /gene="ugpC"
                     /locus_tag="KRH39_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBG8511897.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNNIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT         8401 a         2411 c         3763 g         6884 t
ORIGIN      
        1 atataaatta atattttatt tttgcaacaa atattttgag ataatacaaa ctattaacat
       61 acattttgat agtatttcca ataattataa gataatacaa gaaattactc atttttgtaa
      121 atttaatgaa aaattataaa aatataaatt tatatactta attaagataa tttttccaat
      181 aatcaagata atttttttca ttagtatgta tgatagatgc atcaaacaat ataactaata
      241 tgttagaagg ttatatgtaa ttaatgtcag tgtttatata gaatagcatt tgtagcattt
      301 attatagttg taattaccat ttatatcata gacttgaata gtctaaaaaa atagactata
      361 atatttttat agactaaaga aagggctgat aagatggaca tagcaaaact aattttaaat
      421 cctgcaaggc ttcgtatttt acaatatatt cgacttcata gcagtgttcg cacatctgat
      481 attgtaaagt atttaaatga tattcctcgt gcaacagttt atcaccatgt aaaaatttta
      541 gaagagaaca atatgattga agtaattaaa gaaaatcgtg tgcgtggtac tattgaaaag
      601 gtttatgcac taaaggaata tacaacatct atggaaggag agaccttcgt tgcactttct
      661 acagcatttt atgtaggtct gatgcaggaa atgaatgaat actttagcag aaaagagcaa
      721 gaccataaaa aggataatgt atttttctct tcagcattac tatatatgag tgataatgaa
      781 tatgaaaatc ttcttaaaag tatagctgat ttattgaaac catatataga acaaaaacct
      841 aaatcaaatc taaaacttag aaaactttca attatatctt ctccacctgt agctgataat
      901 gataaaacaa aataacatgc aacccatttt tataaaagga ggttatatat gaaatttcat
      961 gagtttggaa ataaaaacag accacatatt atgttgattc atgggggagg gaatgcatgg
     1021 tggaactatt tgcgacaagc agaagttttg tctaacaaat atcatgtgat acttcctaca
     1081 ttggatggtc atggagaaga atatgaaaca acatacattt ctacagagga tacagcagat
     1141 aagttgatat catatataga tgaaaattgc agtggacatc ttttcgcttt gtgtggtgta
     1201 tcacttggtg gtcaaattgt catggaactt ttatctcgaa aacctaatat tactaaaaaa
     1261 gcaattattg atggtagtat ttgctaccca agacctctta tggcaaggtt ttgtattgct
     1321 ggtattcgat tttttggagg acttttgttt agtgagaagg cttgtcgctt tcaaattgct
     1381 atgatgccaa aattgttacc tgaaaatatg caatatccag atgaaataaa agcgtattat
     1441 atgacagata tgccacatgt aagaaaagaa acattctata acatgtatcg tacttacatg
     1501 atgaactata cacttaaaga gaatgttaaa aaaaccacag cacaagtaat gtactggtat
     1561 ggagaaaaag aaatgaagtg tgtcaaaaaa tctgcaaaac tgtttcagtc ttatgtgcct
     1621 tcttgtgaga tttatgaagc taagagctat aatcatggat atttatcact ttatcttcca
     1681 aatgaatggt tggaaattgc agaaccattt ttccaaaagg aataaaggtg ttaggtgatt
     1741 attgtataat tattgctgtt ttttaatata gtaaataaat taggtataaa tataaaaaag
     1801 ttattttagt taatatatta gtgaaagctt aataggaaaa ttataaatag attaaaggta
     1861 gcgcctatat aaatagttgc tacctttttc tgtttgtaaa taaatgatag ataagaaggt
     1921 ttactgacaa atcactgact aaacccaatt ttcttacatt gaaagcctta attttacttg
     1981 ttttaaataa atttacaatt tcttgttata aaatactaaa ttttgttaac aattataatt
     2041 agcatttaat ttatactgga ggtttatatg attagtgtaa gaacagattt agctttagaa
     2101 gctagtgaaa tgtgtgaaaa gtctcaagaa ggaagtagta ttcctggtgt aaaaatagaa
     2161 actaaagaat tagaaaattg cattgtaact aaagttgaag taatagacag gcaaggcgca
     2221 gagataatga ataaagatat aggaaagtat ataactctgg aaagtaatct tatgaagttt
     2281 gatgatgatg agtcaagaga agaaatgata agttatttaa aagaggagtt agtagatata
     2341 tttggccaag acaaaaataa aaaaaccctg gtaattggtc ttggaaatag aaatattact
     2401 tctgatgcgt taggaccaaa gagtgtatca aaaacacttg taactagaca tctttttaaa
     2461 aattataata aagactatga tgatgatttc actgaggtat cagctttaag cccaggagtt
     2521 atgggtgtta ctggaataga aactagtgaa atagtaaaat cattggttga gaaggttaag
     2581 ccagatagag tagtagcaat agatgcattg gcttcaagaa aaatggagag agtaaactct
     2641 actatacaga tatcaacagc aggaatatca cctggtggag gtgttggcaa tactagaaaa
     2701 tctctaacta aagaaacttt aggcgtggat gttattgcaa taggcgttcc aacagtggtt
     2761 gatgcagcaa ctcttacaat agatgtctta gatatggcaa tagacaattt aatagctcaa
     2821 tcagaagaaa cagaaagttt ttatgaaatg ttgaaaaaac ttaaagaaga agaaaaatat
     2881 catctaataa aagattcgtt agacccatat gacaagaact taatagtaac accaaaggat
     2941 atagatgata caatcgaaaa tctatctata ataataagtg aaggcttaaa taggtcttta
     3001 catccaggta gattggtata aaataaggag gagcaattat gtttaaaaaa tgcattaaag
     3061 tagtaacctt gacttttata ttagtatgca ttttaccagg aaaatcatta gctttgaatc
     3121 aagatgattt tttaaagttt ttagtaaatt catcttatcc agaagctaaa gtcgaaggaa
     3181 atgatacaga aaataaaaag aataataaaa ataaagaaac aagtagggaa aataaggaag
     3241 aaagtaaaga aggaaacact aaatctaaag atgcaagtaa agttgataat aaaaaagaga
     3301 gtgaaaaaga atatataaaa ctttatgttg gtaaagaaaa tgttccagat attgagtcaa
     3361 aaaattcaga cacaactgaa actaacacaa catctagttc agattataag gatgatttaa
     3421 gagtaaccaa agaaaatcca agaattttga tatatcatac tcatggatgt gagacttatt
     3481 caaattcacc tgatggaaat tatcattcaa gagataagaa aaattcagta atggaagtag
     3541 gaagtgcatt aactagtgct ttagatagca aaggatgggg tgtagtccat actactaaat
     3601 accatgacta tccatcttat aataactctt atgcaagtag cttaaaaaca atacaaagta
     3661 tattacctaa atataactca gtggatatag cgatagactt acatagagat gctagggatt
     3721 taactaatcc agccactaag gaaaaagacc atttaaaata tacaactatg ataaatggag
     3781 agagagtatc aaagttcttc tttgttgtag gaggtaaaaa tacaaataga aaacaactta
     3841 gagctttagc agaagatata actgcttttg ctgagaaaaa gtatcctggt ctagtatctc
     3901 caatagtaga aaaagactat gctagattca atcagtttgc agttaagaat catatgttag
     3961 tagaaatagg gaataacgca acaagtgtag aagaatctaa ggctactaca aagtatttag
     4021 ccgagatttt ggatgaatat tttaaacaaa aaaattagag ctttaagtta taattaggag
     4081 catgctaaat gagccgatta gaaaaaaata tagaaagtaa aagtaaacta agaaaataca
     4141 agtatattat gagaatctta tttttgatta ctatgattat atgtacatca gtttgtgtat
     4201 ttatagtgga cagtaatgca aaaagtatgc taggaatgaa acaatatgat tttccaagta
     4261 ttaaatttga tataacaaaa tttgattttg acataccaaa ttttgatata catgaaatca
     4321 taaaaaaatt taataagtag taataagtga ataaggttta ttgattaaaa taagccttat
     4381 tttagttata tatttttaat gtataaaata aaaattcatt aactgtattt tataggattt
     4441 actttaagtt tacgatatta tataatattt agtgttagac ttgataatcc acatataatg
     4501 tggataattt tgtttagtgc caattagttt atattcaaaa attttaaatt tggtatataa
     4561 agagttttta gataatgtaa aataataact ataagaataa tcaagaggag ggaattttaa
     4621 ggtggacaat aaacaaagta gaactagaaa ttttagtata attgcacata tagaccatgg
     4681 aaagtctacc ttagctgata gattaataca acaaactggg cttgttagtg aaagagacat
     4741 gaaaagtcaa ttacttgaca atatggacct tgaaagagaa agaggaatca ctataaagct
     4801 tcaaaacata agattaatgt ataaagcgaa ggatggaaat gaatattatt taaatcttat
     4861 agatacacct ggacatgtag actttaacta tgaggtatca agaagtttag ctgcatgtga
     4921 aggagcacta ttagtagttg atgcagctca aggtgttgag gcacaaactt tagccaatgt
     4981 ttatcttgct atagaccaag atttagaaat attaccaata ataaataaaa tagatttacc
     5041 aagtgcaaga ccagaggaag taaaaaatga aatagaagat ttaatagggc ttgattcaag
     5101 tgaagctcct cttatttcag ctaaaactgg tttaaacata gaagatgttt tagaagatat
     5161 tgtaaaaaat gttcctccac ctaaaggaga taatgaagct ccattaaagg cattaatatt
     5221 cgattcttac tatgatgctt acaaaggtgt tgtagcttat gttagagtat ttgaaggaac
     5281 tgttaaaaag ggtatgacaa taaaaatgat gaataccaat aaaaagtttg aggtaactga
     5341 agttggtgtc atggctccag gtcaaactga acttagtgaa ttatcagcag gtgatgttgg
     5401 ttatattgct gctagtataa aagatataag aagttgtcgt gttggtgata caataacaga
     5461 ttcaaataat cctacagaag aacctttacc aggatacaaa aaagctactc caatggtata
     5521 ttgtggtata tatccaggag aaggagaaaa gtatgagaat gttagagatg ctcttgaaaa
     5581 gcttcaagta aatgatgctg cacttgaata tgaagctgag acttcagcag cactaggatt
     5641 tggttttaga tgtggtttct taggtctttt acatatggag ataatgcaag aaagacttga
     5701 gagagaattt aaccttgata taattactac agctccatct gttatatata gagttacaaa
     5761 aatggatgga gaggttgtaa tgatacaaaa tcctgcaaac ttaccagaac catctgagat
     5821 aaagatgata gaagagccta tagtaaaagg agatataata gttcctaaag actatgtggg
     5881 tgttgttatg gagctttgcc aggaaagacg tggaaatatg ttaaatatgg aatatataga
     5941 tgatagaaga gtaatgcttc actatgacct tcctctcaat gaagttgttt acgatttctt
     6001 tgatgcttta aaatcaagaa ctagaggtta tggttctctt gattatgagg ttaaaggtta
     6061 tgtagcatct acacttgtaa aacttgatat attaatcaat aaagagcaag tggatgcact
     6121 tagttttatt gtccatgaga cacgtgcttt ccctagagga aaggcaatgt gtgagaaatt
     6181 aaaaggagaa attccaagac atcagtttgc tataccaata caagctgctg ttggtaataa
     6241 ggttattgca agagaaacaa taagcgctct aagaaaagat gttcttgcta aatgttatgg
     6301 aggagatata tctcgtaaga agaaacttct tgaaaaacaa aaagaaggta agaagcgtat
     6361 gagacaaatt ggttctgttg aagttcctca aaaagcattt atgtctgtgc ttaaattaga
     6421 tgagtaaatc cttataaatt tagataaaga tatttaaaag ctagagtgat tttagaatta
     6481 tttctaaatt agtctagctt ttttatggta tattttaagt aaactcatag agaatataag
     6541 taagaatatt aaagaagaat aaaaaggtga tatttatgat taatatagaa attcctaaaa
     6601 aagtagacta tataattaaa gaattagaaa agaatggtta tgaagcttat atagttggag
     6661 gatgcgttag agattgttta ttagaaagag ttccaaacga ttgggatata acaactagtg
     6721 ctagaccaga agtggtagta gaattatttg aaaagacaat tccaactggt atacaacatg
     6781 ggactgtaac agttatgatt gaacatgaac catttgaagt gacaacttat agaattgatg
     6841 gaaattatag tgatggtaga catccagatt caatagaatt tacaaataac atagttaagg
     6901 acttgtcaag aagagatttt actatcaatt caattgcata caattcaaag acaggattag
     6961 tcgacccatt taatggctat gaagatatac aaaataagta tatacgttgt gttggaaatc
     7021 cagtagatag atttgaagaa gatgcactta gaatgcttag agcagttaga ttttcagcac
     7081 aactcaattt taaaatagct gaaggtacta agcaaagtat tcataaaaaa gcagatttga
     7141 taaaaaatgt ttctattgaa agaatccaga cagagtttaa taaaatatta gtatctgatt
     7201 catctaaatt aaacttatta aaatcaacag gtttgttgaa atttataatt cctgaaatat
     7261 gtgaattaga agatgttact caacacaatc catatcacat ctatgatgta caaaaacata
     7321 ctttaatagc tacagaagta attgaagatg aactgtattt aaaacttaca atgttgtttc
     7381 atgatttagg taaaaaagta actaaaacaa ctgataaaaa tggtgttgac catttttata
     7441 ctcatagtag agaatctgta aagattgcaa agaagatttt gaaaagactt aagtatgata
     7501 attatactat taataaagtt ttaatattaa tacaatatca tgattataga atagaaccta
     7561 aaaggaagat aattaaaaag ttattaaata aacttgaaga tgttggatta tttgaagatt
     7621 taataaaagt taattgggct gatacacttg ccaaaaatcc aaagtatgca aaacaaaaga
     7681 tattaaatct tattgagtgt gaaaaagaat ttaaacatat aataaatcaa aaggagtgct
     7741 ttaatcttaa agatttggct attaatggaa aagatttgat atctataggt ataaaaccag
     7801 gtaaagatat aggacatatt ttaaataaga tgcttgaaat agtcataaat aatccagagt
     7861 taaatgaaaa agaaatacta aaagaaaaag cattaaatat atatacattt tagattcaaa
     7921 tataaaaaat ttaattactt taaaaaacat agaataaaat acttatttag ataaaataaa
     7981 taatttttag agaatatttt agaagttacg attgtatgat ttttaaatgc ataggcattt
     8041 actctaggac tatagactaa tataaaaaaa aactttggga atactaaaac cataaattta
     8101 taaaattaat attttatata ctgtaaaaca gtgaggaggg acagtatgca tactcaacta
     8161 caaaagacta ttggtctttc agcagctctt tctactgttg taggtatggt tataggttcg
     8221 ggagtttttt ttaagccaca agcaatatac actacaacta atggagctcc aggtctaggt
     8281 attatagcgt ggcttttagg tggttttata acaataactg ctgggcttac agctacagaa
     8341 atttcagctg ctataccaaa aactggtggt atgatgattt atatcgaaga aatatatgga
     8401 gaaaagttag gttttttaac tggatggatg caaactgtat tatttttccc aggtacatca
     8461 gcggcattag gagtaatatt tgctcaacaa gcatctgaac ttttaggaat gagcccaaat
     8521 aatatggcaa atgtacttcc tatagcaatt ggagttatac tatttttagc gttattaaat
     8581 ataataggtt catccttagg aggaaaagtt cagacagtag ccactattgg aaagatgata
     8641 cctttaattt taataatagt ctttggattt attaaaggtc aaagtagtga agtattaaat
     8701 ccatttgtag gagatggtgt aaatgcatca aatgcacttg gacaagtatt aatagctact
     8761 ttgtttgcat atgatggttg gataaatgtt ggagctatat caggagaaat gaagtctcca
     8821 gagaaagatt taccaagagc aatagttggt ggtttatcat tagttatggc agtttatatt
     8881 ataataaata tagcttatct ttgggtagta ccagcaagtg aattagcaac agttacttcc
     8941 ccagctacat tagtagcaac aagattgttt ggaaatatag gtggaaaagt aataactgtg
     9001 ggtatattaa tatcagtatt tgggacattg aatggatatt tattaacagg ttcaaggata
     9061 ccttatacat tagctgaaat gggaacttta ccagcatcta aaactctttt aaaagtaaat
     9121 tctggaggtt ctcctgtaaa ttcaatacta cttataactg tgttagcctg tgtatatgca
     9181 ttatctggtc aatttaatct tttgacagac ttaacaatat tttcaatatg ggtattttat
     9241 gtattgactt ttataggtgt tatgaggctt agaagacaaa agccagattt acacagacca
     9301 tataaagtcc ctttatatcc aataatacca attatagcta tattaggtgg actgtttgta
     9361 ataataaatc aaatattgac ttcaacagtc atatctttag gcggaatatt tataacacta
     9421 ttaggtttac cagtttatta ttatatgaaa aaaagataac tacttaattt aagttagata
     9481 aatttgggta aaggtgaaaa ttaactttta tccaaatttt ttatactgtt atacaatata
     9541 agtattatat attgcttgat gtgctatttt gtttgattta taatttagta attgatgttt
     9601 taagataatt taagaaatga ggagtgaaag atgctaggac tttatgtaca tattccattt
     9661 tgtgtgaaaa aatgtaagta ttgtgatttt aattcttata aaatggacat agattcaaaa
     9721 aaaagatata tagaagactt aaaaatagaa atggaattat atagtaataa attatataaa
     9781 gataataaat ataaaaataa agaatgctgt agtctaaata aaaatgacaa aataacgagt
     9841 atatttgtag ggggaggtac acctagtata ctaacctctg atgaaataag agaagtattt
     9901 ataagtataa aagaaatgtt tgatatagat gagaatgcag aaataactat agagtgtaat
     9961 cctgggacat taacattaga aaaattaaaa actatgaaag agattggcat aaatagactt
    10021 agtatagggc tacaagctat tcaagaaaag catttgaact ttataggaag aatacatact
    10081 tatgaagaat ttgagaaaaa ctataaagat gcattaagtg taggttttaa aaatattaat
    10141 gtagatttaa tgtacagtct tccaaatcaa actctatgtg attggaagga aactcttgaa
    10201 aaagttgtac acttaaatcc aacacatata tcagcttatt cccttatact agaagaagga
    10261 actgaactat acaatatgta tgaaagtaat aaatttgaac ttatagatga aaatgtagat
    10321 atagaaatgt atgagtatac aataaactac ttaaagtcaa aaggatataa tcaatatgaa
    10381 atatcaaact actcaaaaga aggctataat tgtgagcata atattttata ttgggaatgt
    10441 gaacattata tagggattgg agcaggagct tctggctata ttaatgagaa cagatacaat
    10501 aatgtagaat ctttagaaga ttatcattta agtttggtaa aaagagaaaa accaatacaa
    10561 gaaaatgaaa ttctttctga gaaagatatg atagaagaaa agatatttat ggggcttaga
    10621 atgaataaag gcataaaatt tgaagatttt aaaaaaaaat ttggaataga ttttagagag
    10681 aagtataata agcaaataga aatgctatta gctagaaagc ttataaatca gagctttgaa
    10741 ggtatacaat taactcaaaa aggaagagaa atatctaata gtgtatttat agaatttatg
    10801 gaataatttt taaggtgttg acaaaataaa tttataatgg tatataatat atataatctt
    10861 tttagcactc gaacttagtg agtgctaaca gctaggaggt ataataatat ggaattaaat
    10921 gaaagaaaat taaatatcct caaagctata gttaaagatt atatagaaac agctgaagct
    10981 ataggttcta gaacaatatc taaaagacat gatttaggtg ttagtgctgc taccataaga
    11041 aatgaaatgg ctgaccttga agaattaggg tatttaattc agcctcatac ttctgcgggt
    11101 agagttccat ctgaaaaagg ctataagtta tatgtaaact ctcttatgag taaaagcgaa
    11161 ttggatgata atgataagat tcttattgaa caatgtatga atcacaatat aaatcatata
    11221 aaagaattga ttcatgaaac ttctaagcta ttatcgcaac taacaaacta tacaactgta
    11281 gcagttacta agagtttaat aaatcaaagt gttataaagc acatacagtt ggttgctatg
    11341 aatgacaata atattttact tatagttgta acagataaag gggacttaaa aaaagctaac
    11401 cttacaacta atgtatattt agatcaatct aagttaaatt tgatttctga taatcttaca
    11461 aggaagctct taggtaaaag tataacagat ttggatgata atctaattgc ttttattaaa
    11521 tacgaaataa gtgagtattc tgggcttata gatgagcttt taaatgcttt gaactcaaat
    11581 atgaaagaag aagatttttc tctatcatta aatggagcta ctaacatatt tagttatcca
    11641 gaatttaatg atgtattaaa agcaaagtca tttttaaata tgttggaaaa aaaggaaaca
    11701 atagctgaca taattaaatc aaagggaata caaaaagaca atctaaatat aataattggt
    11761 agtgataatg attgtgaact ggcacaggat tgtagtatag ttacagcaac ttataatgta
    11821 gatagagact tggttgggcg aataagcttt ataggcccta ccagaatgga ttatgctaga
    11881 atttactcta taataaatta tatgagttta ttaattaata gaaagtaaag aaattttaaa
    11941 ttgcgaggtg taaagtcttg gataaaaaaa atgagcaaca ggaagtaaga gaagaaaatg
    12001 atacttctat taaccaagaa tcggaaactc aagtagagtt agaagaggaa gttgttaatg
    12061 aagaatgtga aacatcaagt gaaaaaactg atgaaaaaga agttgatgat gaaaatgtaa
    12121 cagatataaa ttctaagtta gcagaaaaaa aattgcagga tgagttggat gaattaaatg
    12181 ataaatatca aagacttcaa gccgaatatg caaactacag aagaagaact caacaagaga
    12241 aagagacaat aggcgtattt gcaaatgaaa aaataataac tgaattgata ccagttatag
    12301 atagtatgga aagagcctta gatgcatgtg aagataagga agatactatg tataagggta
    12361 taagccttgt acataaacaa ttaatagaca ctttagtaaa atttggagta gaagaaatag
    12421 aagcagaatc taaggaattt gaccctaatc tacatctagc agttatgcaa gaaagtgtag
    12481 atggagttga agcaaatcaa atagtaatgg tacttcaaaa aggatataag ctaggaacta
    12541 aagttgtaag accttcaatg gttaaagttt cttgctaagt ataaattaaa ggttaaatta
    12601 tttaacctaa tataaaatat taaaataagt aaaacttaat tatataatta ctcgaggagg
    12661 attttagtca tgggaaaaat aataggaata gatttaggaa caacaaattc atgtgttgca
    12721 gtgttagaag gtggagaagc acaaataata gcaaatagtg aaggtatgag aactactcca
    12781 tcagtagtag catttacaaa agatggtgaa agaatagttg gagaacctgc aaaaagacaa
    12841 gcagttacaa atgcagataa aacaataact tctataaaaa ctcatatggg aactgattat
    12901 aaagtaaata tagatggaaa atcatatact ccacaagaaa tatcagcaat aatattacaa
    12961 aaattaaaat cagatgcaga aagttactta ggacaaacag taacagaagc agttataaca
    13021 gtaccagctt actttacaga tgctcaaaga caagcaacta aagatgctgg tagaatagca
    13081 gggcttgatg ttaagagaat aataaatgag ccaacagcag cagctcttgc ttatggtatg
    13141 gataaattag accaagaaaa gaaaatatta gtatttgact taggtggagg aacttttgac
    13201 gtatctatac ttgaaatagg agatggaact tttgaagttt tagctacagc tggtaacaat
    13261 agattaggtg gagatgactt tgaccaaata gtaatagatt atttagcaga ggaatttaaa
    13321 aaagctgaag gtgtagattt aagaaatgat aaaatggctc ttcaaagatt aaaagaagca
    13381 gcagaaaagg ctaagaaaga attatcatca acaatgagtt caaacataaa cttacctttc
    13441 ataactgcta cagcagaagg accaaaacac ttaaatatag atttatcaag agctaaattt
    13501 gaagaattaa ctagaggttt agttgaaaaa actatggaac caactaagac agctttacaa
    13561 gatgcaggac tttctacagg tgatatagat gatgtattat tagtaggtgg ttctacaaga
    13621 ataccagcag tacaagaagc tgttaagaag tttataggaa aagaacctca taaaggtata
    13681 aacccagatg agtgtgttgc agcaggtgct tctatacaag ctggagtttt agcaggggat
    13741 gttaaagact tattattact agatgttact ccattatctc ttggtataga aactatgggt
    13801 aatgtaatga ctaagataat agaaagaaat actacaatac caactaagaa gtctcaaata
    13861 ttctcaactg ctgctgataa ccaaacagct gttgatatac atgttcttca aggtgaaaga
    13921 agtatggctt atgacaatac aactttagga agattccaat taacagatat accaccagca
    13981 caaagaggaa tacctcaaat agaagttact tttgatatag atgcaaatgg tatagttaat
    14041 gtttcagcta aagatttagg tacaggaaaa gagcaaaaaa taactataac atcaaacact
    14101 aatttatcag aagcagaaat agagcaaaag ataaaagaag cagaaatgaa tgcagaagct
    14161 gacaaacaaa agaaagaaaa aatagaagca ttcaatcaag ctgaatctac tatatatcaa
    14221 actgaaaaaa ctttaaatga acttggagat aaaataagtt caggtgaaaa agaggatata
    14281 gaaaaagcta tagcagactt aaaagctgta aaagataatc aagatgctac agcagaagaa
    14341 cttaagaaag ctacagatga agtaatgact aagttccaaa aagtatctca agaaatgtac
    14401 caaaaagcag ctcaagaaca acaagcggca caaggtgcag aacaagcaca agacaatgga
    14461 cctaaagatg acaatgtagt agatgctgac tttaaagaag tagatgaaga taaataagct
    14521 atataatatg taacactaaa tgataattgg aacatatatg atttgtgaaa atcatataga
    14581 tgttaaagat atatggacaa tatacatcat aaagaatata atatatcata gattatataa
    14641 cttaaagtag aatataagaa ttaatgatag cttgtagttt attattctac aagctatcgt
    14701 tttgtaaaat gaaggtggtg tcaatttttg agtacaaaaa gggattatta tgaagtctta
    14761 ggtatcagta aaggtgcaga agctcaagag ataaaaaaag cttatagaaa attagcaatg
    14821 aagtaccatc cagatagaaa tcctggagat aaagaggcgg aagaaaaatt taaagagata
    14881 aatgaagctt atgaagttct atctgatgat acaaagagaa aaacttatga ccagtttgga
    14941 catgatgggc taaatggtca aggcggtttt ggtggtcaag gaggatttgg cggtcaaggc
    15001 tttggtggtt ttgaagatat gttcggagat atatttggtg acatgtttgg aggaagcttc
    15061 ggaggtggaa gagcaagaag aagagggcct caacgtggtg cagatataag acagagtgtt
    15121 actatatctt ttgaagaagc tgcatttggt aaaaagatgt ctataaaagt aaatagaagt
    15181 gaagagtgtg aagaatgtaa tggaactgga gcaaaaccag gaacatcaaa gaaaacttgt
    15241 tcaacttgta atggaactgg tcaagttaga actgttcaaa gaactccatt tggtaatata
    15301 gcaagttcaa gaccatgtag tgcttgtaat ggaactggag aagtgattga atctccatgt
    15361 agtaaatgtc atggaactgg aaatacaaga aaagttaaga ctatagaagt agatatacct
    15421 gctggtatag atgatggtca aatgataaaa ctttctggtc aaggtgaggt aggagaaaaa
    15481 ggtgctccaa gaggagactt atatattgta gttaatgtta agtcacatcc actatttact
    15541 agagatggaa atgatattta ttttgaaata ccaattacat ttgttcaagc aactttaggt
    15601 gatgagatag aagtacctac tttggatgga aaagttaaat atagtgtgcc agaggggaca
    15661 caaactggaa ctgtatttag acttaaagag aaaggaatcc ctagaattag aggtaattca
    15721 agaggagacc aatatgttaa ggttgtagtt gaaattccta aaaaattaaa cgataagcaa
    15781 aaagaactat tgagagaatt tgcgaaggag tgtgggtcga acgtccacga aaagaagaaa
    15841 accttcggac aaaagataga agatatgttt aagaaaaagt agttaaagta gccaatatat
    15901 aatcatattt ttgaaatctt agttatttat aagattgtca aaggagatat tatgattttt
    15961 atattggcta tttttgtata aaaaataaat gtgcataaaa taaaactgaa tataaataat
    16021 tttctgaaaa atacttgtcc taaaatatat aatatgctat aatataatca aataaaagaa
    16081 aggggtgatt ccagtgggaa cggatgtggc actgccaaat gatgaagaaa actttattgt
    16141 aatatacaaa ataaaattag atacttttag tgctagatta agtactggaa aaactcatga
    16201 gctgggataa ttattgtaat tacctagact caaactttta gtagtaaaat agtaatatta
    16261 tatagacaag gtatctaatt tttaaatctt ttgtatgaat taaaattttt ataaagggta
    16321 tatctgatta gaatgtaatt agggaaacct agttgtaaat tacttttagt atatacatgt
    16381 gtaaagaaat atgtatatgg tattggttat tgtgctagat gtgtatgtaa aggaatatgt
    16441 gtctagttgg gtatatgtaa agaaatatac ttataatcta tgaaaaattt ttagtataaa
    16501 ttgctaaaac attgagattg ttttttatag aaagttagtt tataccaact aaaaatgtgc
    16561 ttaagatttg tttgatgttt aattataaat aaggttgtag ataaaaaaat aagtagagac
    16621 aaaggtttaa aatacttgct ttaaatatag tttgttttga acacttgcca aattaaaata
    16681 attaataaaa tagacatctt ctcatagtaa aatatactgt gagaggatgt ttttgctttg
    16741 ttcataaact tttatgaaaa atttgcatag tgtcacattt taatatacta tatatataat
    16801 aaaatataga taaaattaaa tgattcaaaa gagaaaggtg aaaagagatg tattatatag
    16861 gtgtagacat aggaggaaca ggtatacaag caggagtagt tgataactat gggaaaataa
    16921 tttttaggtc agagtgtaaa acagtaattg aaaaaggatt tgaaggaata cttaatgaca
    16981 taaaaataat gatttataag ttgttggaag ataataaact tactatgagt gacataaaat
    17041 ctattggatt tggtgttcca ggttttataa ataaagaagg attagtaact tgtgtaaatt
    17101 taaaatggaa taaaaaagct tttaataaag agttaaaaag aagatttcca gatgttgaaa
    17161 ttcatggaga aaatgatgca acagtagcag cacttggaga agcaaagttt ggaagtatga
    17221 aaggtgctaa tgttggagta ttatatactt taggaactgg tgtaggtgga ggaatagtaa
    17281 ttaatcaaaa agtcttttct ggagcacatg gattgggttc agaaataggt catcaaatta
    17341 taggagaaaa ctattttaat tgtaattgtg gaaataatgg ttgtgtagaa actttttgtt
    17401 cagcaactgc aattataaag tattctcaaa aactgataga agaaggagaa aagagtagga
    17461 ttttagattt ggcagaggga aatttagaaa atgtaaatgc aaagatggtg tttgatgcat
    17521 atagagaaaa tgatttagtg gcaattaaag taataaatag atttaaagag tatttagcaa
    17581 aaacttttgc aaacacaata aattctttag acccagaaat tatatctata ggaggaggca
    17641 tttctaaatc tagcgatata atacttgatg gaatagaagg tttggtaaga aaatttgtat
    17701 tatacaaaac agaagatata gctacaatta catgtgcaac tttaggttca gacgcaggaa
    17761 ttataggagc agctttttta taatgtaaat gaactgctta ttggaggcgt gatgatatat
    17821 gggtaaaaca aatgctagaa gaacgctttt tttgatagca ataggttcag gaactatgct
    17881 gaatcctctt aattcttcta tgatttcact tgctttacat agtatacaaa atgattttca
    17941 tattagtttt acaactgtat cttggattgt ttcagcattt tacctagcta gtgctattgc
    18001 acaaccagtt tcaggtaaga taggagattt aataggtaga aaggtcttat ttttgagtgg
    18061 tttgatgctt gtaattttat cagcatccat agtaccactt gttcaatcat tctttatatt
    18121 gatttttata agagtaattc aagcaattgg aacaagtacc ctatatccat ctggagttgc
    18181 tcttatacaa aataatataa aagaaagaca atcttctgca ttagcagttc taaccatttt
    18241 tgcttcaact acagcaggat taggtccgac acttggagga ttattgttgg acttaggagg
    18301 atggcatgct atatttcttg taaatatacc tgttgttttg gtttctatat gcttagggta
    18361 ttttttattc ccaaaagaag taaaagaaaa gaaatctata aaagaaactt taaaaaatgt
    18421 tttgtcaaga ttagatatta ttggcatact tctattttca attgcaatga tttttatatt
    18481 actatttttg ctatctatga aggagtcttt taacttggaa caacttgtat ttggtataat
    18541 actgatgtgt ctttttattt ggcatgaatt gagaacaaaa ttgccattta tagatattaa
    18601 gctttttgta agtaatccta aattgtcaaa agtttacttg caatttatta tattaaattt
    18661 ctttaattat attttgtttt ttggtcttcc tagttatttt caagatgcct tacattatag
    18721 tgcaaagtcg actggattat ttatgctatt tatgtctggt ataggtatat ttatatctcc
    18781 attaactgga aaatggatag ataaatctgg aactagattt cctcttgtta catcatcaat
    18841 ctttatgttt attagtgcag tacttctttc agtagttttt atccatatac ctgtaatagg
    18901 aaagggtatt atattatcat tagcaggtat tagttatgga gttggtaatg tagccctgca
    18961 atcctctatg tttgaagaaa gtcctcgtga cagtataggg acagcttctg gtttgtttca
    19021 aactagtcgt tatttaggtt ctattttagc aactgtttta cttggaatgg tatttgaagt
    19081 aaatataaca tcagaacaat ttcagatatt aggttatgtt atggtggtct taggtacaat
    19141 aagtttttta ttaaatttta tattaaagaa agaattaaga aaagtagaat agtggattta
    19201 tggatttaat aagtctaata actgataagt ttaaaaatat tgtttgtaaa acataactgg
    19261 tttgatattt attaattcta agttttgaag catagagtac tcgataagta tacaatattt
    19321 tgaattaaaa ttaagcagaa atttgaacta gtctttcaaa tatctgctta atttagtagt
    19381 taaatttttt tatagagaaa ataagatttg acacttagat aaaaaatcat tgcattttca
    19441 aaattcttta aatcaaaccc aagtatctca ttaatctttt ccaatctata tactagcgtg
    19501 tttctatgta aaaacatact agtagatgtc ttagaaatat ttaaatcatt atttataaat
    19561 tttattcctg tatatagaag ttcttcgtca atagatttaa tcttattaaa atctattaat
    19621 ttattgagta tattatcctc attaattaaa tctaaagttt tatatctaat aaggtcaaac
    19681 aaatttaaaa acttattttc agcgttgtct tttataatat gaggtagtaa atcagaacat
    19741 aactgtaatc taaaatcaaa atcagcttta ctatctaatt caccaagata aaaaatataa
    19801 gtactttgat aagtttcagt ttcaattata ttttgtgtag ttacattaat atctaagtta
    19861 tatttagaat ttactataaa aaaaccattg tgttgtatta tatttatatc ttcaaataaa
    19921 ttataaaaaa tattttttag atattcaaag tctatatgag attggtaaat taaataaaag
    19981 ttgcctttta aattatcaag attattttta tctagaaaat caataacata aatagcatta
    20041 ttgaaagtaa ctaaatggct actagcatct aacttttggt tttcatatgg atagatattt
    20101 atttttttat taagctgtct ttctaaaaaa tcttttaact cttccataaa gtctccttaa
    20161 aaataagatt atattcttat cttatagcta aagtagtttc tttgtcaaat atgtgtactc
    20221 tactattgtc aactgaaagt gttaagtcat caccaacatt aattctgttt ttactgtcaa
    20281 atctaattgt tatcgtattt tcgcctagtt ttaagtgagc ataagtttct gctcccataa
    20341 gttcttttat ttcaattctt gatttaaaac aagtatctgg attagattta ataaaagtat
    20401 cttctacatg aatatcttct ggtctgatac ctataacaac ttcattatta atataattgt
    20461 cttttattaa atcatagtgt ttttctttat tcaatttaag cttaatatgt tcattttgtg
    20521 cataaatttc atcattttct tctattaata ttacatcaat aaaattcatc tgtggtgctc
    20581 caataaatcc tgcaacaaat atattagctg gataatcgta tacatcttgt ggagttgcaa
    20641 tctgctgaac tactccatcc ttcattacta cgattctatc agccatagtc atagcttcaa
    20701 cttggtcgtg tgttacatag ataaatgtag ttccaagctg ttggtgtagc ttagttatct
    20761 ctgttctcat agcagttcta agttttgcat ctaagttgga tagaggttca tccattaaga
    20821 ataccttagg atttctaact attgcacgtc ctaaggcaac cctttgtctt tgtcctccag
    20881 ataatgtttt aggtttttta tttaaaagag gtagtaagtc taatatttta gcagcttctt
    20941 taactttttt gtctatttca tctttaggca attttctaag tttaagtgca aatgccatat
    21001 tttcgtaaac agataagtgg ggatataaag catagttttg aaaaaccata gctatatctc
    21061 tatcttttgg ttctatatta ttgacaagtt tatctccaat gtatagttct ccttcagaga
    21121 tttcttcaag accagcaatc attctaagtg tagtcgattt accacaacca gatggtccta
    21181 ctaaaacgat aaattcttta tctttaatgt ctatatttat attttttact gcgttaaatc
    21241 catttgagta aagttttgat atatttttta aaattacttc tgacataata tcctccttga
    21301 tatttaaatt ttaattatat agtatcaaat ttttttatta tattaaatgt gtaaaatgac
    21361 taaatgtatt tgtttaatat gtatatttag tttttagcaa agaataaaac taacagtaaa
    21421 atatatttaa aaaattaaaa aacaaaatta aatttatta
//
LOCUS       DAEORZ010000058        21068 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEORZ010000058 DAEORZ010000000
VERSION     DAEORZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.058"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            154..1578
                     /locus_tag="KRH39_001996"
     CDS             154..1578
                     /locus_tag="KRH39_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBG8511898.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1613..3742
                     /locus_tag="KRH39_001997"
     CDS             1613..3742
                     /locus_tag="KRH39_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG8511899.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNEKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQTQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            3772..4194
                     /locus_tag="KRH39_001998"
     CDS             3772..4194
                     /locus_tag="KRH39_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8511900.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4213..5364
                     /locus_tag="KRH39_001999"
     CDS             4213..5364
                     /locus_tag="KRH39_001999"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG8511901.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEGILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5470..5571
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5754..6326
                     /locus_tag="KRH39_002000"
     CDS             5754..6326
                     /locus_tag="KRH39_002000"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBG8511902.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6390..7040
                     /gene="fsa"
                     /locus_tag="KRH39_002001"
     CDS             6390..7040
                     /gene="fsa"
                     /locus_tag="KRH39_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG8511903.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7192..10116
                     /locus_tag="KRH39_002002"
     CDS             7192..10116
                     /locus_tag="KRH39_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8511904.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            10705..11163
                     /locus_tag="KRH39_002003"
     CDS             10705..11163
                     /locus_tag="KRH39_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8511905.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            11224..11499
                     /locus_tag="KRH39_002004"
     CDS             11224..11499
                     /locus_tag="KRH39_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8511906.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            11558..12832
                     /locus_tag="KRH39_002005"
     CDS             11558..12832
                     /locus_tag="KRH39_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG8511907.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            12962..14014
                     /locus_tag="KRH39_002006"
     CDS             12962..14014
                     /locus_tag="KRH39_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG8511908.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            14014..15045
                     /locus_tag="KRH39_002007"
     CDS             14014..15045
                     /locus_tag="KRH39_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBG8511909.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            15137..15949
                     /locus_tag="KRH39_002008"
     CDS             15137..15949
                     /locus_tag="KRH39_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG8511910.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            15952..16872
                     /locus_tag="KRH39_002009"
     CDS             15952..16872
                     /locus_tag="KRH39_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG8511911.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            17047..17493
                     /gene="rpiB"
                     /locus_tag="KRH39_002010"
     CDS             17047..17493
                     /gene="rpiB"
                     /locus_tag="KRH39_002010"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG8511912.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            17523..18188
                     /locus_tag="KRH39_002011"
     CDS             17523..18188
                     /locus_tag="KRH39_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG8511913.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            18298..19800
                     /locus_tag="KRH39_002012"
     CDS             18298..19800
                     /locus_tag="KRH39_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBG8511914.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(20112..>21068)
                     /locus_tag="KRH39_002013"
     CDS             complement(20112..>21068)
                     /locus_tag="KRH39_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8511915.1"
                     /translation="LVLIFFMYIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYIN
                     NISKNIKNIENGNLKYKLEVIGDDEISTLAKSINNITGGLETAVDANIKNERMKAELI
                     TNVSHDLKTPLTSILSYVDMLKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKL
                     SSGDVEFNITKTDIRELLIQSIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVF
                     ENLICNILKYSMPNTRVYIDMFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISR
                     KTDGSGLGLSIAQNIIELENAHMEIIIDADLFKVMLTFRRVN"
BASE COUNT         8524 a         2217 c         3919 g         6408 t
ORIGIN      
        1 attaaacaaa aactaaaatg acaaatgtca ttatctaaag tgacatagaa acggtttctt
       61 aaagttgtta ctataaaata taaactctat aataaaagta taacaaaagg gaatcatatc
      121 gatatagaat ataattcaag aggaggtaaa attatgagta gttctaatca atcgacaata
      181 aagacatcta tacagcgagt agggaaattt ttaagtggta tggtaatgcc aaatatagga
      241 gcttttatag catggggtct tataacagca ttatttatac caacaggatg gatgcctaat
      301 gaaaaattga gtactatagg tgacccaatg ataaaatacc ttttaccact attgatagct
      361 tatactggtg gtaaagcaat agcaggacaa agaggtggcg taataggtgc agcagcagct
      421 atgggagtaa ttgttggtgc agatatacca atgtttatcg gagcaatgat aatgggacca
      481 tttgcaggat gggtaattaa gaagtttgat aaatttgtag atggaaagat accaacagga
      541 tttgaaatgt tagtaaataa cttttcaata ggtataatag gtatgcttct tgcaatacta
      601 ggattttatg caattggacc agcaatagta gcagggacag ctcttataga atctggagtt
      661 caatttatag ttagcaaaag tttattgcct ctagtttcag tatttataga acctggaaaa
      721 gtattattct taaacaatgc aataaatcat ggtatattag ggcctatagg tatagctgag
      781 gcaaaagaag caggtaaatc tataatgttc ttactagaaa gtaaccctgg accaggttta
      841 ggagttctat tagcatattg gatgtttagt agaggaagtg taaaacaatc agcaccagga
      901 gctgtaataa tacatttctt tggtggtatc catgaaattt acttcccata tattttaatg
      961 aatccagttt taatattggc tacaatggca ggtggagcag caggaatatt gactttttca
     1021 atattaggag ctgggcttgt agcagcacca tcaccaggaa gtatatttgc acttatggca
     1081 ctagctccaa agggaggatt acttccagta ttagcaggag ttgcagtggc aacagttgta
     1141 tcattcttag tggcagcacc atttgttaag agagcttcag caaatcaaag tgaagaagac
     1201 agtacttctt tagaagaagc taaagcaaaa atgagtgata tgaaatctgc aagtaagaac
     1261 tctgagaaaa atattgagga aaaacagctt gaagtaaatg agattaagaa gattgtattt
     1321 gcatgtgatg caggaatggg ttcgagtgca atgggagctt ccagatttaa aaatagaata
     1381 aaaaacttag acttaaatat agaaattaca aattcatctg ttgataatct tccagatgat
     1441 acacaaatag tagtaactca caatacatta gttgaaagag ttgctaagaa taattcaagt
     1501 gttgagatag tttcaataaa caatttttta aatgacccaa atttagatac attatttaaa
     1561 agattagaat ctaaataaaa ataaaaagta ccactaatta ggagtggata atttgaaaaa
     1621 aaagaagatt acatctaggc aaaaaaagat aatactaatg atagtagaaa attcaaagaa
     1681 aaacattcca ataactattt ctgagattgc agggacttta gagttaagtt caagaactgt
     1741 acttagagat atgtctggaa ttgaaaaatg gtttgatgaa aatgatttta actttgtaaa
     1801 aaaaccagga gtgggattga ttttagagga aaatatagaa aatcaaaact ttataattga
     1861 gttgttagaa gaagaaaaaa tagaaaaaga atacagcaag gaagaaagaa atttaattat
     1921 attatcaaag ctactagttt ctaacgagcc agtaaaatcc tattattttg caaaaatatt
     1981 aaaggtatct gaaggggtac tgaataatga ttttgcctta gcaagtaaat ggttggaaag
     2041 atttgatatt gagcttgtta gaaaaccagg gcttggtgtc tacttaaaag gtcaagaaaa
     2101 aaactttaga gaggcatatg taaatcttat atacgattca ttcaatgaaa aagaaatact
     2161 agatatggtc agaaatataa gtgaaaatat acaaacagat aaagcaatag aaattttaag
     2221 tgaaaataga cttttaaatt taatggacag gtgcataata agaaaagttg aagctacatt
     2281 aacaaagaag ctttcagatt tagatgtaaa tttagctgat agtgcatata tagggcttgt
     2341 agttcatatt tcattagcct tacagaggat aaaaaatggt gaaaatataa ctatggataa
     2401 agagtttcta aaagaattat ctataacaga agagtttaaa ttagctaaag aaatcgtaaa
     2461 aggtatggaa acagatttta acatggatat accagtagat gaggttggat atataactat
     2521 gcatataaga ggtgcaaagc aaaggtcatc ttcaaaccat aaggctttaa acttagatga
     2581 tatagaaata atggaaatta caaataagat gatagatttg gcggaagatg aatttaaaat
     2641 aagtttaaaa aatgacgaga ggttatttaa agacctagca aatcacttag ggccttctat
     2701 aaataggttg aatatgggat tagaaataag aaatcctctt ttagatgaaa taaaaagcaa
     2761 gtattcatat gcctatgatg gagttgaaaa gatttctaga attataaaag ataaactaaa
     2821 tataaattca atacctgaat cagaaatagg atatattgca atgcactttg catcagccat
     2881 agaaaaaaat cttatgatga atactaatat aaatattgtt gtggcatgtc ccactggaat
     2941 aggtacttca agattcttat caactaagat tgaaaataaa tttcctaatt taaatatact
     3001 tgaaaccata tcagctataa acatagatga agagtattta aaggaaaaag atgttgattt
     3061 aatagtgtct acagttgagt taaatacaag tttaaattat atatgtgtag gtccatttat
     3121 gagtttagat gatgaacaga taatcaagga aaaaataaaa tctatagctc aaaataaatt
     3181 aatcaattta aatgagaaaa atgatactaa gagtaagaat aaagtatatg aacagataac
     3241 agaaagtatg aatataggaa aagatattct tcaattttta gaggaaatta gatttgaaaa
     3301 atttgaaagt aaagatttaa gtgagcttat tgaagactca tcaaaaatat ttgcaaagtc
     3361 tagtgaagat ataatctcta taaaagaatc gttaaaagaa agattaaaaa tttcaattcc
     3421 atatatagaa gaatcaaaaa ttctattgtt acattgtatg agtgaaagaa tagatattat
     3481 gaagttatca ataataaaac tagagaatag aatcgtgcta gattcaaatg aagaaatcga
     3541 caatgtagtt tttatgttac ttcctaaaaa ttcaccaagt tatcaaacac aaataatgag
     3601 tgaaataagt ggttcattaa tagacaatat aatttttact aataagataa ataaattctc
     3661 tatagaagag atggcattag aggtaaaaga tatagttttt aatttttata caaacagatt
     3721 aaaagcattt atagataaat aaaattaata ttaaaaagga aggttgatac tatgagtaaa
     3781 gtattgaatg agaataatat atttttagga ttagatagtg tttcaaagga agaagctata
     3841 actttagcag gaagaaaatt agttgaaaat ggatatgtaa aagaagaata tataccagca
     3901 atgttagaaa gagaaaaggt aatgactaca tatatgggaa tgggagttgc tattccacat
     3961 ggtgtaaatg aagcaaaaaa agaaatatta tcttctggaa tagttatatt acaattccct
     4021 aatggtatag attttgatgg agaaaaagca tatttattaa taggaatagc tggtgttgga
     4081 gatgaacatt tagaaatact atctaatata gcagtagttt tagatgatga cttaacagaa
     4141 agactaaaaa attcaaatga caaacaagct tttatggaag cttttgccaa ctaatcttag
     4201 gagggattta aaatgaaaaa ggcaattcag tttggagcag gaaatattgg aagaggtttt
     4261 attggaggtt tgttagttaa gtcgggttat catgtagtat ttgctgatgt aaatgagggg
     4321 atattaaact caataaacaa agataaaaag tatactatac acataagaga tgtagaatgt
     4381 atagatgaag taattgataa tataagtgcg gtttcatcta taaaagaaga aataatagat
     4441 gagattgtac aggctgaaat aataactaca gcagttggac ctttagtttt gactaaaata
     4501 gcatctacaa ttgcaaaagg tataaaagct agaaaagaaa agggattaac aagtaatcta
     4561 aatataatag catgtgaaaa tgcaatatac gcaagttctt ctttaaaaga agaggtactt
     4621 aagtatttaa ataaggaaga agtagaatat ttagagatgt atgttggttt tccaaactgt
     4681 tcagttgata gaatagttcc tcctggtaaa aacgaaaatc ctcttgatgt aacagttgaa
     4741 aatttttatg agtggaatgt tgaaaaacaa ggatttaaag gggaaatacc aactatagtt
     4801 ggaatgaatt tagctgataa tcttatggct tatatagaac gtaaattgtt tacactgaat
     4861 acaggtcatg ctataacagc ttatatagga tatttaaaag gatataagac tatagaagag
     4921 agtataaaag ataaatttat ctgtgatata gtcaaaagtg caatggtaga aagtggagaa
     4981 ggattgatta aaaaatacaa ttttgactca gaagtgcact ataaatatat tgataaaatt
     5041 ttaaatagat ttaaaaatcc atatcttaat gatgatgtat taagagtcgg aagggaacca
     5101 ttaagaaaac taagtgataa agatagattg attaaacctt taatgacagc aaaatcatat
     5161 ggattatcag ttgataattt gattcttgga ataggtgcag cgcttcatta taataatagt
     5221 gaagatactc aaagtgtaga attacaagag ttgataaagt ctattggggt aaaaaaagcc
     5281 gttgctaaaa tagctaatat aagcaatgac gaagaacttt taaataatat cgaaaaaagt
     5341 tatatattta tgaaaaattt ataaatttac ttgaaaaaaa gtagttctga taaagtaact
     5401 ataatatatt attctacaca gtttgaatta tttgttgaat aatagagaat tataatatat
     5461 aattaaaagg aataaattaa tatatttacc tgtatatgtt tgataatatg gttcaaatgt
     5521 ttctactaag ctaccattaa tagcttgact acaggagtga agaagatata gtttatactt
     5581 attttaaatt atatattaag ttaattactt tgaattttaa gctcctgtct atgatataga
     5641 caggagcttt tttagtattt taatataaaa tacttacaag aatagatagc aaaattagaa
     5701 caaatataat ttttttatta atataatttt agaaagggct aggtggaagt tcaatgaagg
     5761 ctttacaaga aaagatattg agagaaggca gtgtttcagg aaatgatata ctaaaagttg
     5821 atagtttttt aaaccatcaa attgatgtag cttttttaaa tgaaatagga agagaattta
     5881 aagagagatt taaaggagaa aaggtggata agatatttac aatagaggct tcaggaatag
     5941 ctatagcatc aattgtttca cagtattttg ataatgcacc tgtagtattt gcaaaaaaat
     6001 cagaatctaa aaatttggat aaagatgtat atgaaactaa tgtatattca tttactaagg
     6061 caagagagta ttcagttaaa gtttctaaaa agtacataaa caaaggcgaa aatatattga
     6121 tagtggatga ttttttggca aatggaagag ctgctcttgg acttaaagat ttaattgagc
     6181 aagcagaagc taacttagtt ggagtaggaa ttgtcattga aaaaggattt caagctggag
     6241 gagcactttt aaaggctaat gatgtaagat tagagtcttt ggcagtagtt gaatctatag
     6301 ataatggaac tgtaaaattt aggtaattta tgatattata tacttgtgtt tagcacatat
     6361 tttaactatg taattatgga ggatttttta tgagattttt tatagacaca gccaatattg
     6421 aagaaataaa agaagcaaat gatttaggtg taatctgtgg tgtaactact aatccgtctc
     6481 taatagctaa agaaggtaga gattttatag aggtagttaa agaaataagt gaaatagtag
     6541 atggaccaat aagtgcagaa gtaataagtt tagaacataa aggtatgata gaggaagcag
     6601 aaaaattatc taagatacat aaaaatatag ttataaagat acctatgaca gcagaaggtc
     6661 taaaagcggt gaaagtacta tcaagtaaag gtataaagac taatgtaacg cttatattct
     6721 ctgctggaca agctctttta gctgctagag ctggagctac atatgtaagt ccttttgttg
     6781 gtagattgga tgatatatca caaaatggtc ttgatttaat tgaagaaatc gttgatatat
     6841 ttagtgtaaa tagtattgag gctcaaataa tagttgctag tgttagaaat ccaatacatg
     6901 ttttacaagc tgctagaatg ggagcagata tagcaacagt tcctcttaaa gttataaacc
     6961 aaatgattaa gcatcctttg acagacaaag gaatagatag ctttatgaag gattgggaag
     7021 gtgcttcttt aaaattataa ttataaacta cgctttataa gtatattaaa gaggttatta
     7081 tggaaattct aagtaataag gctaggctat tttgcctagt cttattttgg tgtataatat
     7141 attgaaacat attattgaaa tagattaatt ttttaagtga ggaggcgaaa aatggataaa
     7201 atattaagag agatacaaag agaagataaa aaaaatccat atactgacca agaacttgct
     7261 gaaatgttaa atgtagctag gtctgaagtg atatcagtta ggaaaaaaaa taatatttta
     7321 gattcgagag aaagaagaaa aaaaatatta ataaaagata tatcaaaaat tgtaagtgaa
     7381 aatcctcaaa tgtctgaaag aaaaataact gaaatattaa tagaaaatgg atataatata
     7441 tcgagaagtg cagtttcaaa attattaaaa gaggaaaatt taaatcagtc aaagattggg
     7501 attaaggata aagacccagc atcatcagga aagtatgtga aggtgagtaa agttgtagaa
     7561 gaagatggat ttgaagaatt gataggtata aagggaagtt tgaaagaaaa ggtaaatcta
     7621 gctaagtcag caataatgta tcctcctaat ggccttcata ctataatata tggagaaaca
     7681 ggagtaggta aaagtgaatt agctacatgt atgtataaat atgctataaa aaacaacatt
     7741 aaagaagaga acagcccatt tatagtgttt aactgtgcag actatgcaga aaatcctaat
     7801 ctattgatag ctcaactctt tggggtagta aaaggtgcat atacaggagc agatgcaaat
     7861 agagaaggtc ttgtagaaca agctaataat ggaatattgt ttttagatga aattcataga
     7921 ttgcctgctg ctgggcaaga aatcttattt tcattaatag atagaggaga atttagaagg
     7981 ttaggagaaa gtagttctaa tagaaaggca aatgttctca taataagtgc tacaacagaa
     8041 aatccagatt ctaatttact tcaaacattt agaagacgta taccaatggt aataaattta
     8101 cctaatatga gtgaaagacc aaaatctgaa agatatgaaa taataataaa gttttttgaa
     8161 agagaagcaa agcgagtaaa caagaatttt ataataacta aagaagttat gtgtgcactt
     8221 atgaattata agtgtactgg aaatataggt cagttaaaga gtgatataca agtaacttgt
     8281 gctagagctt ttagtaaatc gatattttct agcgataaag taattataga tttagatagt
     8341 ttaagggatt atataaaaag tggatattat gattgtaatt gtaaaaatga aaagtattct
     8401 gagttttcag ttgaggatat atatatagat attagtgaat tggtgagtgg aaaaaataga
     8461 attttatctg ataatgagat aagcgagata tataattatg cagaaaaaga gcttaaggtt
     8521 ttggaaagta agtgcttttc agaagaagag ttaaaaaatg catttataga aaagttagat
     8581 aagaagttta atgctattaa aaacaataaa aatttatctg atagaagaag tcgaatagac
     8641 tggggaggac aattaaaaga gtctacactg gagataatgg ataaggtaat tgtatttatt
     8701 aaggagcagt ttaagcatgt aaatcaaggt ctgtacttgg ctctagcaat tcatattgag
     8761 catgctatag gtaggataaa agatggaaaa actataataa atccgagttt agataaaata
     8821 aaagcatcaa tgccaatgga atatgagttg tcaagatata taatgggtat agtagaagat
     8881 ttgacacaag taacatttcc agaagatgag cttggatatt tggcttatta tataaataaa
     8941 ttttgttata atgaggaaag tataaaagat aaagttaaag tagtaatagt tacgcatggt
     9001 aaagttggaa ttgaaatgtc taaagttgta aatcatattt taggtataga atgtacacta
     9061 ggtatagaaa tagcacttac agattcacca tcagagggga tagagcatgt attagaagag
     9121 cttcaaaaga tagaagctag aaaaggtatt ctagttctaa ttgatatggg ttctttggta
     9181 attcttggtg atgaggttga aaaaaggctt ggaattagat gtaaaactgt aaatagagta
     9241 gatacattat tggcaatgga agcaggaaaa cttgcaacca ttgaagggaa aagtttagat
     9301 ggtataatag ctgatttaaa gaaaaataag aattatgcta tgataaatac aaataaattt
     9361 agttacagaa aaaacgagta tggaaagaaa aatgtaatca ttactttatg tttgagtggt
     9421 gtaggtacag ctttaaattt aaaagagcat atagaaaaac aaattaagga atatgacact
     9481 ttaattgaag taaagccagt tgctttttta aataataatt tagaagaaga gttaaatgat
     9541 attgaagctg aatataatat tattgctatc tgtggaacta ttgatataga ttataaaaac
     9601 gtgcctttta tttcttataa tgaggtactt ggtaaaaatg gtattaataa agtattagag
     9661 catttaaata gtaaacaacc agctttaaat attgaaaacc agttgaataa tttaatacat
     9721 gaagatttga ttttatacaa ttttgaagga atttcaaagg agtatataat tgatactctt
     9781 gtttcaaaac ttgaagaagg tggatatgtc gactcaaagt atatattaag tgtatataaa
     9841 agagaagcaa tgggaagtgt tgtaatggcc tctaaggtag ccgtgccaca tggacttcca
     9901 gaaaacgtta taaagcctgc aatagcaatt gcgaggttga ataaacctat agtatgggac
     9961 aataaattta tggtggattt ggtggtacta ctggcactta aagaaaataa taagaaagaa
    10021 atacgaagct tattttcaaa gattaatgac cagcatactt tagaagtttt attaaatact
    10081 gaagataaaa atgaaataaa agaactttta agctaattgt tagtaccgag aagaaagaca
    10141 aaaaaatttg ctttagtata aaatttaaaa ctactcaatt gctaagtagt taattactct
    10201 atataaggct agttctatga aatttattta tatgatttta tagaattagc ttttttcttg
    10261 gtaaataaaa tatatgaatt tatttatgta aaaagtaaat tttattggac aattctatga
    10321 gaatttttcc tatatttctt ataaaatata gaatatgtaa aaaatatata aaaataaatt
    10381 aaattcataa taaataattt tataaaatag ttgttaaaca taaaaaacca tttgtaaaaa
    10441 acaaaaaaat catttctcta acaaaaaaaa ccacaaaaaa tataaaaaag tatggctagt
    10501 taagtgatta acaagtaaaa attaaaataa aaatcaaaaa aaatttatca atgaaagtca
    10561 gctaaaatcc tggaagagag tattattttt gggaaaacat tttcttttat ttcacaaaaa
    10621 aagccacttt ttggcacaca acatgcttta tataaagtca agaaaaacta aggtataaga
    10681 caaaggcaat taggaggtgt aaatatggag tttataaatt ttgtaagtga taaagttata
    10741 gaccttaaaa tggattgtaa aacgaaagaa gatttcttta aagaaataca taataaagtt
    10801 tttgatttag gctttgtaaa agaagaattt ggagaaaaaa tattagcaag agaaaatgta
    10861 tttccaacag gtttaaattt aggtgattat ggagtagcaa taccacatac agatgcagaa
    10921 tatattaaag agcaatttat atcagtctgt acttttaatg aaccagtagt atttagttct
    10981 atggaagacc aagatgaaaa agtacctgta aatttggctt tcgtattagg acttaatcaa
    11041 ccacacagtc aattatctgt attaacagaa ttaatgggaa ttatgcaaaa taaagagtta
    11101 gttgataagt tgatgagttc tactgataaa gaagaagttt taaagacact aaagtcttta
    11161 taaaaataaa aaagtactaa aaataaaaaa tataaaaata aaaattatta ggaggaaata
    11221 aaaatgaaaa aaatattagt agcatgtgga gcaggaatag caacatcaac agtagtttgt
    11281 gacaaggtag agagattagt taaggaaaat aatatacaag cagaagttat acaatgcaaa
    11341 atagctgaat gtgcaacaaa gcaagaagga gcagatttaa tagtatcaac aactatacta
    11401 ccaacaactt ataatatacc agcaataaaa gcaacagcat atataactgg agtaaataca
    11461 gcagcattag ataaaaaaat attagatgct ttaaaataat cctatcatag atacattgaa
    11521 ataatatttt ataaaacaga aaaaggagga tttataaatg gataaattat tagcagtagt
    11581 acaatacatt ttaaatatgg gaccgacagt aatactacca ataacaattg tagtactagg
    11641 aatgatattt agaattaagt ttacaaaagc tttaaagtca ggtcttacaa ttggtatagg
    11701 atttgtgggt ataaacttag ttgtaaacct attaacatca acactagggc cagcggctca
    11761 aggaatggtt gaaagattcg gacttaattt aacagtaata gatgttggtt ggccaacaac
    11821 agcatcagca acatgggctt caccagttgc accaatatta ataccagttt gtttattagt
    11881 aaacttacta ttattatact tcaataaaac aaagatatta aacgttgata tatggaatta
    11941 ttggcacttt atagtagcag gtgcagttgg atatatagta acaggaagtt atgtttgggc
    12001 tatattctgt gcaatactaa tggagttatt agtactgttt attgcagaaa agacagcaaa
    12061 aaatgttcag gagttttatg atttagaagg tatagcatta ccaactggtt caacagcttc
    12121 atttgctcca cttggatatt tagtaggtaa atttgttgaa aaaataccag gaataaataa
    12181 aatacaagca gacccagact ctatacaaaa aagatttggt atatttggag aaccaatgat
    12241 gatggggatt atactaggta taatacttgg agcacttgcg ggatatgatg tagcaggaat
    12301 attcaatgta ggtatatcaa tgggtgcagt aatgttactt atgcctagaa tggttagaat
    12361 attaatggaa ggtttaattc caatttcaga atcaataaga gatttcttac aagcaaaata
    12421 tggagacaga gaactttata taggtctgga tgcagcagta gcaactggac atccagcagt
    12481 tatatcaaca gctcttatac ttgttcctat aactttgttc ttagcagtta tactaccagg
    12541 aaacaaagtt ttaccatttg gagacttagc gacaatacca ttttatatgg catttatagt
    12601 gtgttttaga aaaggtaata tagttcaatc agttataaca ggaacaatag taatagctat
    12661 atcactttat atggctacaa actttgcaga tgttcataca caattattac aacaagcaaa
    12721 tatgatgcca gaaggaacaa caatggtcac aagtatagat acaggtggaa acttgctaaa
    12781 atggggaata ttgaaattat ctcaattagt ttcttcattt ggaatattct aatatgtagt
    12841 taatagagat tactcttttt tagtaaagta tgcgaaaatt taaaatttag cagctactaa
    12901 aaaagagtaa tattttttat aaatttttaa aaatacaaaa tacaagagga ggagatttat
    12961 cgtgaaagca gcagtattac atggcacaaa tgatatgaga tttgaagata tagaaataaa
    13021 accatgtgaa agtgatgaag taaaaataaa agttatggca gcaggaatat gtgggtcaga
    13081 tcctccaaga gtattaaaac attggaaata tccagttcca gctataccag gacatgagtt
    13141 ttctggagta attgctgaag ttggtaagga tgtaaaaaat gtaaaagttg gagatagagt
    13201 agttgcaatt ccttttatac cttgtaatga atgtgaatat tgtaaaagag gattattttc
    13261 attgtgtgat gaccatggaa tgttaggagc taaaagtttt ggagcttttg cagagtatgt
    13321 aaatataaaa gctacaaatg tacttccaat aggagatatg gactttgaag atgctgcaat
    13381 gatagagcca ctagcagttg ctatgcatgg agtacttaat ataggtgttc aagttggaga
    13441 cactgtagcc gtaatgggta gtggaacaat ggggcagttg gtaatacaag gacttaaaat
    13501 agcaggggca ggaacaatta tagcagttga tatatctgat aataaattaa gagaatcaaa
    13561 ggaattaggg gctgatataa taattaatgc taaagatatt aacccagtag aaaaaataaa
    13621 agaacttact ggtggaaaag gtgttgacat agctcttgaa tgtgcaggtt caaaaataac
    13681 tcaagaacaa tgtttactta taactaaaaa gaaaagtaag ataggatttt tagggatagc
    13741 atattcagat ataactttat cagaagaagc atttgaaaat atatttagaa aagaactaga
    13801 attaaaaggt ttttggaact cttattctgc accatttcca ggacaagaat ggactaaggg
    13861 aatcaatcta gttaatgaag gaaagataaa attaaaagaa atggtatctc atagattctc
    13921 acttgaagat acttacaaag catttgaaat gataagagat agaaaagaag aatttaacaa
    13981 aatattaatt ttaccacaag gggttgaaaa ataatgaaag cagtagttaa aactaaacca
    14041 ggctatgata atgtagaagt tttagaagta gaagaaccga aagctacagg agacaaggtt
    14101 aaaataaaag tagaatatag tggtatatgt ggttcagata tacattcatt taaaggtgaa
    14161 tatgcaaata taaaagcccc agtaacatta ggtcatgaat tttctggaat tgttgtagaa
    14221 gtaggagaag atgttaaaaa tataaaagta ggagatagag ttacatcaga gactactttt
    14281 gcaacttgtg agaaatgtat atattgtgaa actaaagatt ataacttatg cccaactaga
    14341 aaaggtattg gtactcaagt aaatggaagt tttgctgagt atgtattatc aagagaagaa
    14401 agcatacatg tacttcctga aaatgtatct ttattatcag cagcccttac agagccacta
    14461 gcttgttgtg ttcatgcagc tcttgaaaag actactatag aaagttctga tacagtttta
    14521 ataataggac caggaccaat tggtttatta ttagctcaag ttgttaaatc tcaaggtgct
    14581 acagttataa tgtctggtgt tacaaaagat aaggaaagat tagaaatagc taaaaatctt
    14641 ggagtagata gaactgtaaa tgtaatggaa gaaagtttgg cagatgtagt gaatgaaatg
    14701 actgatggaa atggagtaaa caaaggattt gattgttctg ggtttatgcc agcagttaac
    14761 gaagccttaa gactgcttag aaaaaaaggt actttcgtac aagttggaat ttttgctaaa
    14821 aaattaaatg aaatggacca agaagcaata atccaaagag agttacaata tataggaagt
    14881 agatctcaaa agccaagttc ttggattaca gcattagatt taatggcaag tgggaaagta
    14941 aatacagaag ctttagttac tagaactgtt ggtttagacg aatttaaaga aggtattaca
    15001 gctttaatga acggggaaga aataaaagta gcagtaaaat cttaaaaaaa catttgtatt
    15061 attttaaaaa aaattgtaaa atctactgta ggataaaaaa atgtaaggtc ttaaaaaata
    15121 acaggaggaa gaaagtatga gcactcttag agatcattct aataatatta gaaaaaacat
    15181 aataaaaatg gttgcagaag ctaaaagcgg acatccaggt ggttcattat caatagctga
    15241 tatgttgaca gtattatatt ttgataagat gaacgtatgt gcagaaaatc caaaaatggc
    15301 tgatagagat agatttgttc tatcaaaagg tcatgcagca ccagctttat atgctacatt
    15361 agcagaaaaa ggatttttcc cagaagaaga attaataaca ctaagaaaat ttggttctaa
    15421 gttacaaggt cacccagaca tgaaaaaagt agcaggaatc gatatgtcta ctggttctct
    15481 tggtcaaggg ctatctgcag caaatggtat ggcacttgca ggaaaattag ataacaaaga
    15541 ctatacagta tatactatat taggtgatgg agaaatccaa gaaggtcaaa tatgggaagc
    15601 tgcaatgaca gcatcacatt acaaattaga taatttaatt gcatttgttg acttaaatgg
    15661 attacaaata gatggttcta atgaagaagt aatgaatgta aatccagtag atgacaaatt
    15721 caaatctttt ggatggaatg taatagtaat aaatggacat tcttttgaag aaataggaaa
    15781 tgcagttgat gaagctaaaa aagttacagg aaaaccaact gtaataatag ctaaaacagt
    15841 aaaaggtaaa ggtgtttcat ttatggaaaa taatgctgca tggcatggaa cagctccaaa
    15901 tgcagaacaa gcagagcaag cattgaaaga tttagaagga ggtctatagg aatgggaata
    15961 gcaactagag aagcttacgg acaagtgtta aaagaacttg cagaaaataa agatatagta
    16021 gtattagatg cagatttagg aaaagctaca aaaagtatat catttaaaga agttgcccca
    16081 gatagatttt ttgacatggg gatagcagaa ggtgacatga ttggtacagc agcaggtcta
    16141 gctacttgtg gaaaaatacc ttttgcgagt acttttgcaa tatttgcagc aggaagaggc
    16201 tatgagcaaa taagaaactc tgtagcatat ccaaatttaa atgtaaaaat agcagctact
    16261 catgcaggtg taacagtagg tgaagatggt ggtagccatc aagctataga ggatatatct
    16321 ctaatgagag gtataccaaa tatggttgta ttaaatccag cagatgcttt agaagcaaga
    16381 caagctattc ttgcatctat tgattataat ggacctgttt atataagatt aggtagagct
    16441 gcaactcctg atgtaaacag tgaaaactat aaatttgaaa taggaaaagg aactgtttta
    16501 agagaaggtt ctgacataac agtaatagct acaggaataa tggtagcaaa agccttagaa
    16561 gcagcagaag aacttgctaa agaaggtata aatgtagaag ttgttaatat atctacaata
    16621 aaaccattag atgaagcatt aataaaagaa actgctaaga aaactggaaa agtagttaca
    16681 gcagaggagc acagtataat aggtggttta ggttcagctg tatgtgaagc tttagcagaa
    16741 actaaagatg tagtagttag aagaattggt gttaaagatg tatttggtca atcaggtaca
    16801 ccagctgatt tattaaaaca ctatggttta actacagaag atatagtaaa aaatataaaa
    16861 gagttgttat agatataaat atatctaaat aacaaattga tttgacataa gtcaattaaa
    16921 gctgtcttaa aaatagaaag aaatttctac aatgggacag ctttattttt acaatacttt
    16981 atttttatga taattataaa atagtctgaa agtaatcaaa aaaattataa aaagaggtgc
    17041 tatttaatga aaatatctat tggatgtgac catggtggtt atgaattaaa ggaatatata
    17101 aaggaaaatt taataaataa aggtatagaa gttgaagatg tgggtacaaa ttcaaaagaa
    17161 agtgtagatt ttccaatata tgcaaaaaag gtaagtcaaa atgttcaaag taaaaaatct
    17221 gatttaggca tactttgttg tggaacagga ataggtatgt ctattgcagc aaataagttt
    17281 aaaggaatca gagcagctgt tgtttctgac tgtttttcag ctaaggcaac tagagaacac
    17341 aataatacaa atgtactttg tttaggagaa agagttattg ggaaaggatt agcaatgaga
    17401 attgttgaag aatggttaaa ttcaagttat aatggagaaa gacatgcaag aagattacaa
    17461 atgattgatg atatagaaaa agaaaattta taaatatata atttataaca agggggagcg
    17521 ttatgtctat tatttgtgct tcaataatgt gtgcagacca aatgaagtta aaagaggaat
    17581 tagaagctct tgaagaagca ggtgtaaaat tacttcattg tgatgttatg gatggaatat
    17641 ttgttaaaaa cttggctatg ggaccagagt tattaaaatc aataagtgaa aatacaacaa
    17701 tacctttaga catacactta gccacagaaa ctccagataa atatattgat atgatgtctt
    17761 atataaagcc aaaatatatt tcttttcatg tagaatcaag tacaaatgta aaagctgata
    17821 tacaaaagtt aagaaattat ggtatagggc cagtgttagc aataagccct caaacaagtg
    17881 tggataaaat agaagaatat ataagtttag ttgagggtat cttagtaatg acagtaaatc
    17941 cagggtttgc aggtcaaaag tttaatcttt ctgtattgga taagttggat aaacttacag
    18001 agatactaaa agattatgat aatcctccat ttatagaggt tgatggaaat ataaataagg
    18061 atactattaa acttatgaat ggtaaaaaag tagatatata tgttgttggt acttcagctt
    18121 tatttaatga taagcctcca atttcatata aagataaaat agaagaacta aaagaaagta
    18181 taaagtaacc atgtaataaa attataatat gtttctgttt aatagtaaaa atttaggtgg
    18241 aaacaagaaa ttttataata tttaataaaa attttggttg agtaaggaga attttttatg
    18301 ggagacctat ataaactaca aaatggtact gatattagag gaatagcgta tgaaaatgat
    18361 tctaaagaag ttaacctaac tgtagaagaa gtaaaaaaaa tagctaaagc gtttcatatt
    18421 tggttaaaag aaaagactaa gaaagataaa gtaactgttg ccataggaac tgattctagg
    18481 ataacaggaa gccaatttag aagtactgtt attgaaactt taactaatga cgattgtaat
    18541 gtaattgatt gtgagattgc cacaactcca gctatgttta tgacaactat tatggatgga
    18601 tacaattctg atggggcgat tatgattaca gcaagtcact taccttatta ctataatggt
    18661 ttaaagtttt tcacagaaag tggaggttta gaaaaaactg atataaaaga gatgttggat
    18721 atagctgtta aaaatgatag tgtagactat gaggaagtaa ataaaaaagg tgaagttata
    18781 gtaaaaaatt taatagaaga ttattctaat ttattgatag ataaaattag aaaaggtgta
    18841 aattcaagta aaaattatga aaagcctttt tctgggctta agatattggt tgatgcagga
    18901 aatggggctg gtggattctt tgctgaaaaa gttttacaca tactgggagc agatacgaca
    18961 ggtagtcagt ttttgaaccc agatggtatg ttcccaaatc atatacctaa tcccgaaaac
    19021 aaagaagcaa tggaaagcat atgtaaagct gtgttggata ataagagcga cctaggaata
    19081 atctttgata cagatgtaga cagagctgct attgtaggta aaaatggcaa accaataaat
    19141 aaaaatgctc ttatagctgt tatatcatca atagtattag aagaacatcc aaaaacagct
    19201 atagtaacag attcgattac ttctgaagga cttgctaaat ttataaatga attacaagga
    19261 agacatcata gatttaaaag aggatataaa aatgtaataa atgaagctat aagattgaac
    19321 agtgaaggtg aagagtgcca tttagctata gaaacgtctg gacatgctgc tttaaaagaa
    19381 aattatttct tagatgatgg ggcatattta atagctaaaa ttttaataaa agtagcaaag
    19441 ctatccttgg aaggaaagac tatagaagaa ttgatagaaa accttgaaga agctcaagaa
    19501 gagaaagaag ttagaatagg cattaataaa gaagatttta gaccatacgc agaggatata
    19561 ttaaaagatt tagattctca tgttaagagt attgatggat ggtctgtagc accaaataat
    19621 tttgaaggca taagagttaa ttgtgatgaa tcaaatggag atggatggtt tttacttaga
    19681 atatcactac atgaaccatt attagcttta aatattgaat caaataaaat tggcggagca
    19741 gaacaaattt attctaagtt aaaacttttc ttagaaaaat atgatttaaa aggtctttag
    19801 ggtaaataga aagaatatat ttatattgaa gaacaaatgt aaatttgtac ctaagattac
    19861 aaatattaca aaattgtaat cttaggtaac agagatgtaa ctgattatgg aaacactatg
    19921 tgatattata gatacagaga gatgaaatat gtgatgaatt atagagaaat ataatttaaa
    19981 atgtattaat atcacaccaa aaaggtataa tcaaaaaata tgtaaatata aaccccaaca
    20041 tttagaaaaa gacctctaag gaggtttttt tcttgtttaa ataacttaaa atatattttt
    20101 atattaatat atcaattaac tcttctaaaa gtaagcatta ccttaaataa atcagcatct
    20161 ataattattt ccatgtgtgc attttctaat tcaattatat tttgagcaat tgataaacca
    20221 agaccagagc catctgtttt tctagatata tcaccccttc taaatctttc tattaactct
    20281 tctggcttta gatttagttt atcatttgaa atatttttaa acgttaatat tatattttct
    20341 tcctttataa acatatctat atagacccta gtgttaggca tagagtattt tagtatattg
    20401 caaattagat tctcaaaaac tctccaagtt tttttaccat ctatcatggt aaaaacagca
    20461 tattcaggtg tttctattat aaaatctaaa ctgctatttt gaattttgtc ttcaaattct
    20521 acaatacttt gaattaaaag ttctcttata tcggtttttg ttatattaaa ttctacatct
    20581 ccactactta gcttagatgc ttcaaatatg tcatctatta ataattttaa tctttgtgat
    20641 tttttatcta aaatatttat atagtcattt atagtctctc tgtcaaagtc attatttttt
    20701 aacatatcaa catagcttaa tatagatgta agtggagttt ttaagtcatg agatacgttt
    20761 gtgataagct ctgccttcat cctttcattt tttatattag catctacagc agtttcaaga
    20821 ccacctgtta tattatttat acttttggct aaagtagata tttcatcatc tcctataact
    20881 tctaatttat actttaaatt gccattttct atatttttta tattttttga tatgttattt
    20941 atgtaattaa acttaacaga taaaaagttg tatatataaa aatgaaaaac tgtgtttaca
    21001 agtgtaaata aaattactat agctattgcc tcagcataat atatatacat aaagaatatc
    21061 aaaacaag
//
LOCUS       DAEORZ010000059        20970 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEORZ010000059 DAEORZ010000000
VERSION     DAEORZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.059"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(290..520)
                     /locus_tag="KRH39_002014"
     CDS             complement(290..520)
                     /locus_tag="KRH39_002014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511916.1"
                     /translation="MKKYTKDLHIMSERNSYSKTDNDATFMRMKEDHMKNGQLKPAYN
                     IQFGVDSEYIVLVSSGHQPTDTITLIPFLKSI"
     gene            complement(1185..2504)
                     /locus_tag="KRH39_002015"
     CDS             complement(1185..2504)
                     /locus_tag="KRH39_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG8511917.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(2545..3291)
                     /locus_tag="KRH39_002016"
     CDS             complement(2545..3291)
                     /locus_tag="KRH39_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG8511918.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(3535..5544)
                     /locus_tag="KRH39_002017"
     CDS             complement(3535..5544)
                     /locus_tag="KRH39_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8511919.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGEASLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEAMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(5982..7415)
                     /locus_tag="KRH39_002018"
     CDS             complement(5982..7415)
                     /locus_tag="KRH39_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG8511920.1"
                     /translation="MSFKKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(7431..8855)
                     /gene="ascB"
                     /locus_tag="KRH39_002019"
     CDS             complement(7431..8855)
                     /gene="ascB"
                     /locus_tag="KRH39_002019"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG8511921.1"
                     /translation="MALRKDFLWGGATAANQCEGGYKEDGRGLANVDVTPQGKDRFPV
                     LAGKMKMFECDDEHYYPAHEAIDMYHNYKEDIKLFAEMGFKCYRMSIAWTRIFPTGVE
                     DVPNEEGLKFYEDLFDECLKYGIEPVVTICHFDVPMYLVKKYGAWRNRKLVDLYVKYC
                     EVIFTRFKGKVKYWLTFNEINMILHLPFIGAGLYIEEGENEEQIKYQAAHHQLVASAL
                     VTKLAHNINQEFKIGCMLAAGNTYPNTCNPLDVWKSIEKDRENYFFIDVQSRGEYPTY
                     ALKELERKGINIVMEEDDKKILKENTVDFISFSYYSSRLTSADPKVNILTEGNVFASL
                     KNPYLDASEWGWQIDPLGLRITMNSLYDRYQKPLFIVENGLGAVDNPDENGNINDDYR
                     IDYLRAHIKAMIDAVELDGVDLWGYTPWGCIDLVSASTGEMKKRYGFIYVDKDNDGNG
                     TLKRSKKKSFAWYKKVIESNGEEL"
     gene            complement(8942..10858)
                     /locus_tag="KRH39_002020"
     CDS             complement(8942..10858)
                     /locus_tag="KRH39_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8511922.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQELITVLA"
     gene            complement(10932..11792)
                     /locus_tag="KRH39_002021"
     CDS             complement(10932..11792)
                     /locus_tag="KRH39_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG8511923.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(12143..13330)
                     /locus_tag="KRH39_002022"
     CDS             complement(12143..13330)
                     /locus_tag="KRH39_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG8511924.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFDNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(13344..14735)
                     /locus_tag="KRH39_002023"
     CDS             complement(13344..14735)
                     /locus_tag="KRH39_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBG8511925.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(15330..17030)
                     /locus_tag="KRH39_002024"
     CDS             complement(15330..17030)
                     /locus_tag="KRH39_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG8511926.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(17002..18405)
                     /locus_tag="KRH39_002025"
     CDS             complement(17002..18405)
                     /locus_tag="KRH39_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG8511927.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEVELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(18670..19161)
                     /locus_tag="KRH39_002026"
     CDS             complement(18670..19161)
                     /locus_tag="KRH39_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8511928.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEADLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(19187..19999)
                     /locus_tag="KRH39_002027"
     CDS             complement(19187..19999)
                     /locus_tag="KRH39_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG8511929.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(20012..>20970)
                     /locus_tag="KRH39_002028"
     CDS             complement(20012..>20970)
                     /locus_tag="KRH39_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG8511930.1"
                     /translation="VEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTAKVLALTF
                     GLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVISRGSEKNT
                     IFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANNFTEIMSEKSM
                     ELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGGVTHIPHG
                     EALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIESFLKRVGL
                     KTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
BASE COUNT         6535 a         4001 c         2172 g         8262 t
ORIGIN      
        1 tttaaaacag ccttttaaat tactttactt aaatattttt ttgtttaact tatttttata
       61 agtaataaac atataaacat cacttatgta aattaatgat ttttaaaata aagatatttt
      121 tattttttat ataaacagta cagaataaaa aaatatttgt gttttccctt ttactattct
      181 attttaaaaa tattttattt attttatatt tctctaaaaa tgtatagttt tcatcacttt
      241 catacccaga atcatccact attttaggat atttaaactt tgtatggaac taaatagatt
      301 ttaaaaatgg tattaatgtt atagtatcag tgggttgatg tcctgaactt accaaaacta
      361 tatattctga atctacacca aattgtatat tatatgctgg tttcaattgc ccatttttca
      421 tatgatcttc tttcattctc ataaaagttg cgtcattatc tgttttagaa taactatttc
      481 gttcactcat tatatgaagg tctttagtat attttttcaa tctatcaata caatcattta
      541 attgctctat agatttgttc tttccctttc catgaacaaa tgttatattt gattcttttg
      601 atatttttta accttcttta aaaatttcct taaatgatgg attttaattt tatctttata
      661 aattatttta ataccaaaat caatctcaca ttttaatatg aagtcagata tattattcat
      721 cattttagcc atatttttat ttatagcttt tttcaaacaa aagtatatct attagcagat
      781 gactctattt ctcttgaaga ataaatacag ttaatgtttg tatatattat aattttcaaa
      841 agttgtctag gggttactct atttcctcta attcgagcat aagtcttata taatttttct
      901 aaattcatct actcaataat ttgacttaat aaccttacag aatcatcttt tggaatcatg
      961 taatcaatac ttagtggtaa tttaaattga taattatcgc cataagtagt ataattaaac
     1021 tatataccat tttgttttaa cacacaatta ttatacaaga ctcccaaact cttttgaact
     1081 tgggattctt gtataataaa gggtgtagca ctattttcta gtgatacagc cccatttccc
     1141 taaattcaaa gtaatattta tagaatctct atagattaca ataattattt gacttctaca
     1201 tcattatatt tctttgaata tactttagtc atatagaaat atataggaac tacaattatc
     1261 atcccaccta atccccaaac tatcttcata ggttcatcaa tacctgcttt tatcagtaat
     1321 aatatactta taataactgc aaatattggt ataattgctc ctccaggtat tctaaaattt
     1381 acatctttac cagcatactt ctttctcaat attagtacag ctagacatgt aggaatatat
     1441 tgaataaatc tagatacaac acttatttga gataatgttg tgaatgaacc cgaccatgct
     1501 attagtaaag ttcctatgac agatactata attgctatat aaggagctcc atttttatta
     1561 gtttttctta tagacttagg cattaatcct tgttcaacca aagcagcagc agaccttggt
     1621 gtaaatatgg atgatgcaat atttatacct ccaacagata ctatagttcc tattgtaatt
     1681 atatacccgc ctataggacc taacatctta atggcagcat ctgcaacagg agtagctgac
     1741 cctgctaagc cattacccaa cacccctata cttattccca gtataagcat atatattact
     1801 gatactacaa acattaccat taccaatgct ttaggtacat ttttcttagg gttctccata
     1861 tcttttgctg caacagctaa agattcaaat cctgtaaaag cataaaataa agttatagat
     1921 actgctacaa aagctgatga cgcactttga cctttttgta ttattacaaa tggggtaaaa
     1981 ttagaaccct ttataaagaa taatcctaca gctataaata aaactattgg taataatttt
     2041 cctatagtca tgatattatt taagtgctta gattgtttca ttcccataaa atttataatc
     2101 ccaagcccta caactaatat accaattatt atatttttaa taagtggttc tgctgcttgt
     2161 ggccatacag atgatagtgt tacagcaaag aaatttgcca tagtagccca agcaatcata
     2221 ctaactatcc acttcataaa tccaacttca aatccaacaa aatctccaaa tgcctctttt
     2281 gcataaagat agggccctcc atcagtatca aaccaactac ctgcttcagc aaaacataaa
     2341 gcaatagaaa cagctaaaag catacaaaaa actaatacta atatacttgc tggacctata
     2401 tctgtataag catcaccagg taacccgaat atacctgacc ctattattgc attgattcct
     2461 aatagtacaa tactccaaaa acctaactta atgccttttt ccattgttat tccccccaaa
     2521 gccaacttaa tatatttata ttttttattt agatgcttct ttaactattg ccataaatat
     2581 ttttttcata ttatcataat ctctagtcat catttctgga tgccattgta tccctattac
     2641 aaactcactt ccttcctttt ctatagcttc aattaaaccg tctttagatg tagctgaaac
     2701 aatatatccc ggtgctactt tgttgacaac taagtgatga aaactattaa ctaaaacttc
     2761 tttttctcct agtatttcat ataattttgt cccctccttt atctgtactg tatgtgtagg
     2821 tacattagat agatgttgct gattgtgttt aatataagca ccttcaatta aagataaatc
     2881 ttgatacaaa ctacctcctt cagttacatt tataatttgt tctcctctac atatacctaa
     2941 aactggtttt ttcatatcta aagcatgttt taataatttc atatcaaaac tatctctctt
     3001 tggtgatatt gcccccaact tattatgagg ttcttctttc catatgagtg gatttacatc
     3061 ttgtccccca gatagtataa gcgcatctat attagatacc tgctccttta taatctcatc
     3121 atcatataca atcggtacaa tatatggtat agctttacac ataactactg attgtatata
     3181 atcattattt acataagccc tttcatagcc aggaaacatt cctccttcat caattagtaa
     3241 attacctgat attcctatta ttggatattt ctttttagcc aatttcttca ccccttaact
     3301 tatttgtgaa caataaaaaa tgctacttct cttaaagagc aagtagcata ccaagatttt
     3361 atttatttga acattataat ttattttaga aatacttttt ttgaatacga gtaatataaa
     3421 taactttatt tacttatatt ttattttaat cctgtttagt ttgacttttt taaattgttt
     3481 taagtacaac taataggtaa aataggcatt tagattttcc tttttatcaa cctattaaat
     3541 actgtttaat atattttttc cccttctatt taattcactt ccctttctac cagattcaca
     3601 tattatcaaa tccagctctt ttaacatatt aagtattccc cttacctcac cttctgttat
     3661 attgtagcct aattcattta ttgaattaac aataccattt ctacctatat ttttattaag
     3721 attattggca ttttttaaac actccattac taactttact ttctctatag atgttctatc
     3781 ctttaatata cttatttctt tactaaaatc ttcagttata tttaataagt tatgaggtaa
     3841 atcatttatt aaaataacat cctcagaact cataatgtta atatacatag cagtattttt
     3901 taattctctt atattacctg gccattcata tgcattaata atgtcttgaa catcttgact
     3961 aattgctctc ttccttttca taaaatgctt taacatagct tctatatctt cttttcgctc
     4021 tcttaaaggt ggtatattta taggcaatac atttatccta tagtataaat ctgctctaaa
     4081 tctatctcct tttatcattt ctaataaatt tttatttgta gccgcaatta ctctaacatc
     4141 tatatctatt acattttctc ctccaaccct cattacttga ttctcttgta atactctaag
     4201 taatttgctt tgtaagtata taggcatgtc tcctatttca tccaaaaata tagttccatt
     4261 gttagcctgt tcaaataatc cttttttacc tccttttaaa gctcctgtaa aagcaccttc
     4321 ttcatatcca aaaagttcac tttcaagtaa attttctggc atagctgcac agtttatcgc
     4381 tataaatggc tgactatgtc ttggtgatgc attatgaata gactgagcca tgagttcttt
     4441 tcccgttcca ctttctcccg ttataagaat tgtataatca gattcagcta ttttcatacc
     4501 taatttctta gttctttcca tcttcgttga aactgttttt atatcatcaa aagtatatct
     4561 agcaacttgt cccttttctc taagcttctt tgataggttt tgctccaatt ttcttatgta
     4621 agttatttct tgaatacaaa aataatatcc agtaaccctt ccaaaactct ctatattata
     4681 cttatttacg tttaagtact tattattaaa ttttataact tcatcatgtg ccttactcaa
     4741 actaaaaatc tttttaatat tctcattctc taattcatct atatttttcc ctataatatt
     4801 actttgaata tctagtatat cccctaaact attattgcaa ataattatgt ttcccttatc
     4861 tgataccatt gccataccta agtttgataa atttattatg gaatttagtt catctgcttt
     4921 tatagtaagt tctttgtatt tagtatttat tcctgaatat aaactaatta tttcatctgt
     4981 atacttaatt agcctttcag taatttcttt atcatccagt ttgagttttg ctattatttg
     5041 aataaacgtt gaactatcaa tatacctatg gcctaaatca ataacttctt ctatatattt
     5101 tggaactaaa cttgcctctc ctggcgttat agctatctta atatttctat attcttcatt
     5161 gggattgtat ggtataaaat tcagatgatt tattcctata ttgtataaat tagatattgt
     5221 ttccaataca gtatgcttat tatcgtttac tactaataca tctattcctt ctggcaatga
     5281 aaataactta tatatatcct tttgctttat gctcctattt atagttatta tctttgagac
     5341 atcttctaca tattttttta tttttaatac tctatcatct atcattacca atattatgtc
     5401 atcttttatt aattccttct ctgttatttt atttaaataa taatttttta tcattacttt
     5461 atctttaaat atcatttcta gatttttatt taagaaacat tctaatttag acaacccatc
     5521 agttactact gctattgttt tcatttgatt actcctttta aaatatactc tactctttta
     5581 taatatacat ttgattttca ctttaataac acaaaaaatt gtacatttag tatactaaga
     5641 tattccttta ttagttttat gtccataaat atcatagttg caatatctat catatttctt
     5701 aaacttctat aaagcaaaaa ttgtgccaaa ttaataattt tcttaatttt attctaaagt
     5761 tcctcaatag tatttacaga cttagcactt atagcttata agtctttata ttattaatct
     5821 atttttatag gttaattgta caaatcatat atcactttta cccattaaat gcattataaa
     5881 aaagatggaa taacgattcg tcactccacc ttttttgtaa taaaaaattg tatatataaa
     5941 cactatttac tactcttcta agtacaaaca gtatctacat actataacct ttcaccatta
     6001 gattctatta cttgtttata ccagtcaaaa ctctttttct taatcctaga caagtctcca
     6061 cttccatcat catacttatc cacgtatata aacccatatc tcttggccat ctcaccagta
     6121 gatgcactta ctaaatctat acatccccat ggagtatatc caaataaatc aacaccatct
     6181 tctacagctt ccttcatttg ctctatatgt gcttttaaat actctatacg atagtcatca
     6241 tttatcactc cattagattc ttttttatca taagctccta aaccattttc aactaccatc
     6301 ataggtattc tatatctatc ataaatttca tttagtgcat atctaagccc ttcagggtct
     6361 atttgccatc cccattcact agacttaaga tatggatttt tcactccacc taatatgttg
     6421 ccacttgtat tatcttcttt agaatcacta cttacacatg tagacatata ataactaaat
     6481 gtataaaaat ctacacaacc ttcttttaaa atttctaaat ctcctttttc catttttatt
     6541 tcaatattat tttctccaaa atatctattc atataagttg gatattcgcc acgcacttgt
     6601 acatctccac agaaataatt cattactcta ctatctcttt gagttttaac tgcatcatct
     6661 ggattacaag tataaggata cattgttgca aatatttgca tacatccaac ttgataattt
     6721 gaatctattt catgagctaa cttaacagcc ttagcacttg ctacaaattg atgatgtaac
     6781 ccttgaaaac gaagttgagg tatatctact tggtttataa aatctgtagt cccttcattt
     6841 aatattcctt gtgataagta gcctcccata ggcattgtag ctgaatttat ttcattaaat
     6901 gtcagccaat attttacttt tcctttatat cttttaaata ttgtttcaca gtaattcata
     6961 tacaagtcta ttacatctct tgaaacccat ccattgtatt tctctgttat tgcaaatggt
     7021 atttcatagt gagatattgt tattaatggt tctatattat attttaaaca ttcatctatc
     7081 acattttcat aaaatttaag tcctgcttca tttggttctt tctccattcc agttggaaat
     7141 attctagtcc atgctattga aaatctaaat accttaaatc ccatttctgc aaataaagct
     7201 atatcttctt tgtatctgtg ataaaaatct attgcttcat gacttggata aaatgttcct
     7261 ttttctataa ctctcgttat tcttttactt actttttgag aacctccagt acacatatct
     7321 gaacaagaag gacctttccc atctaaattc catgctcctt cacattgatt tgctgctgtt
     7381 gctccacccc ataaaaaatc ttttttaaat gacataccta tatcctccta ttataattct
     7441 tcaccatttg attctattac ttttttatac caggcaaaac tcttcttttt acttcgtttt
     7501 aaagttccat ttccatcatt atctttatcc acatatataa atccatagcg cttcttcatt
     7561 tctccagtac ttgcacttac taagtcaata catccccatg gtgtgtaccc ccataaatca
     7621 acaccatcta attcaacagc atctatcatg gcttttatat gagcacgtaa ataatcaatt
     7681 ctataatcat catttatatt tccattttca tctggattat ctactgctcc tagtccattt
     7741 tcaactataa ataatggttt ttgataacgg tcatataaag agttcatagt tattctaagc
     7801 cctagtgggt ctatttgcca tccccactca cttgcatcta gatatggatt tttaagactt
     7861 gcaaaaacat ttccttctgt taaaatattt acttttgggt ctgcacttgt aagtcttgat
     7921 gagtaatatg agaacgaaat aaaatctact gtgttctctt taagtatctt tttatcatct
     7981 tcttccatca ctatatttat tccttttctc tccaattctt ttaatgcata tgttggatat
     8041 tctccacgag actgtacatc tataaaaaag tagttttccc tatctttttc tatagatttc
     8101 catacatcta gtgggttaca agtattagga taagtatttc ctgctgctaa catacaccct
     8161 attttaaatt cttgatttat gttatgtgct agtttagtaa ctaacgcact cgcaactaat
     8221 tgatgatgtg cagcttggta ttttatttgt tcttcatttt ctccctcttc aatatagaga
     8281 cctgcaccta taaatggtaa atgaagtatc atgtttattt cattaaatgt tagccaatac
     8341 tttaccttac ctttaaacct tgtaaaaata acttcacagt attttacata taagtctact
     8401 agcttacgat ttctccatgc tccatatttt tttaccaaat acatgggaac atcaaaatga
     8461 cagattgtta ctactggttc tattccatac tttaaacact catcaaataa atcttcatag
     8521 aattttagtc cctcttcatt tggaacatcc tcaactcctg ttggaaatat cctagtccat
     8581 gctattgaca tacgatagca tttaaatccc atctcagcaa ataattttat atcttcttta
     8641 taattgtgat acatatctat tgcttcatga gctgggtaat agtgttcatc atcacattca
     8701 aacattttca ttttcccagc caaaactgga aatctatctt ttccttgcgg tgtaacgtca
     8761 acattagcta gacctctacc atcttcttta tatccacctt cacattgatt tgctgctgtt
     8821 gctccacccc ataaaaaatc ttttctaagt gccataattc tacctccaat aatttattta
     8881 tagtaacttt attttaaaat aaaaatacat atatataatt cttaattaat agaactctct
     8941 tttacgctaa aacagttatc aattcctgac tattatttac atgcttttgt gtattttcta
     9001 caatatccac ataatcagaa taattagtaa caactactgg tgtttctaag caatatcctt
     9061 cttttgaaat actttctatg tcaaaagtta ttaatgtctg acctttttta atcttatccc
     9121 cttgattaac aaaagatttg aaatatttac cttccaactg aatagtattt aaccctatat
     9181 gtatcaatag ttcacaacca ttatcagata taattcctat tgcatgtttt gttggaaata
     9241 aagtcattac agtcccatca aatggtgcca ctacttttcc ttctttaggt gttattgcta
     9301 caccttttcc caatatacct gttgaaaacg ctgcatcttg tatctgctcc aaagccttta
     9361 cttcaccttt tattgggcta tatactactt ctcttttgtt tttttctata acgccttgcc
     9421 cattatcatc tttagaaatt tctactatag catcatcttt ccaaaaaaat aatgttaaaa
     9481 caaatcctac tacaatagct actattattg ctattatagt tgcataaaca cctttcatat
     9541 ctccagttgt tggatttata taattaggta taccaaatat tccaagtcca cccattacat
     9601 aactttttac attcattatc cccattacag ctcctgccac agcaccacct acacaactat
     9661 atatgaatgg tgtttttctt ggtaaagtta ttccatatat tgcaggctct gttacaccac
     9721 atatccctga aataactgca ggtatacaca gctcttttat cttcttatct tttaatttaa
     9781 agtacattgc tgctactact gctgtttgtg caaaagatgg tacaaatgct cccactaata
     9841 ttgtgtcaaa ccccatagtc ataaggtttg acatagctat tggtactaat ccccaatgta
     9901 gaccaaatat tacaagcact tgccacaata aaccagttaa tgcaccacat aaaattggtg
     9961 aaaagttaaa taaagctaaa aatccatttc caactatatt agaagcatag gtagctatag
    10021 gccctataac cataaatccc aatggtaaag aaactaataa aactgccatt ggtacaaaga
    10081 aattctttac tacatctggt attattttag caaacacttt ctccaactta gctgctaata
    10141 atactattaa aattactggt actactgttg aagtgtagtt cattgctatt actggtaatc
    10201 ctaagaaagt tatatatact ggtgattcaa atattgttcc agtaaataga ttgtataaag
    10261 gttgacctgt agatgctaga gtatctaatt gaatacctga atagcataaa actgcaccta
    10321 taagcattcc aataaatggt tttaatttga atttcttagc agctgtatat ccaagaatta
    10381 ttggcatata ctggaacatt gcatctccaa ttgcatttaa cataatatat gcaccatcag
    10441 tttctccata caaacccaat gctgtaaata ctgcattaaa tccttttatc ataccagcag
    10501 cacacagaac tcctaatatt ggttgaaaaa ctcctgatat tatgtctata aacttattaa
    10561 gtaatccttt tggcgcatct gagtctgaat tatctattgt atctcctcct aatccagcca
    10621 aatcgcacac aacttcataa acatctggaa catgattacc aataactact tggtattgac
    10681 ctgcactttt cattacagta actataccat ccattttttt tagcacatca tcatttgcta
    10741 ttgactcatc ttttaattta aaacgtagcc tcgttataca gtgagataaa ctgctaatat
    10801 tatcctttcc tcctacattc gcaatgattt cttttgatag ttgttcatac tttctcattt
    10861 tttctcctcc attttctttt tgaaatatga aggtttggcc aacttaatgt caccacccaa
    10921 tatatataaa cctaattatt aggatttata tctgacttaa tacttttact taccactctt
    10981 tcaacgtgaa tagttaagta taatttttcc tcatctgtta aatcgtagtt gtatttttta
    11041 tgtataaatt catccaactt ttctatgaat ttataagcat ttttatattt aacttttatt
    11101 acatttaata aatcatcttc attattttca tgtaaattat tactaacaat tcgttctgca
    11161 aaatacttta agtgagtaat aaatctatga taataaactg aatcttcatc aaatactatt
    11221 ctgtaatggt atttaaccat atttgaaatt tcttgtatta tttgtgtaat ctcatatata
    11281 gtttttatac tttgatccat ttgtgcatta actatatgca gtgctataaa tccagcttca
    11341 tcttctgata gtctaatttc aaattttttt tctatataat ccaatgcctc taaccctatt
    11401 ttaaattcac tcttataaaa tctttttata tcccaaagta atacattttt tacacttact
    11461 ccttcacgag ctctttctat tgcagtatac atatggtctg ttaaagaaat ataaatacta
    11521 tcatttaatc ttttccctag tttttcttta gcatataaaa ttatttcatc tgataattcc
    11581 atatatctta taggaatatc tgctattagt tctttaaatt tatcagatat atttgggtct
    11641 gaaagtttga atatcttatc aattttatcc tttgatatat gctctcctgt acgtttttta
    11701 taagctaaac ctctccccat aactactatt tccttatgat tttcatcagt tgtaataatt
    11761 acattattat tgagtatttt ttcaataatc attgtgatgc cccctttatt agcattaagt
    11821 taaaataaat cttttttaat tatttagaat atttaaaatg taatttatta cataaaaaaa
    11881 aacctattta ttacacatta aatagactcc catgagtcct gagtaataaa taggtttagc
    11941 ttgtatttaa acaatcacta tccgtttgta ttttatttta attattattt attatatgtt
    12001 agcaaattta ttttttagtg tcaacttttt ctcaatattt taattttaaa taaattatat
    12061 aaattgaatc ttcaaagtac tatttttatc aataaacaaa ataaaaaagg tattactttc
    12121 tttaagtaat acccatttat ttttaattta agtggttttt cttaatctca tcaagtagtc
    12181 catcttctaa ataaatttct aatgcacatc ctaccattgc ctttactgct ttgtgaagtt
    12241 tatcataagc ttcctcagaa tttgcataat ttagaaattc tttttcatga acatatacca
    12301 ttggacttat atctaaggct atttctgtat acatagtagg gcaaacttga ctgacattac
    12361 ctatatctgt agaacctact ggaccatctt taccttctaa tatatctgtt atacctgcct
    12421 caatcaaatt attctttgtt atcttttgaa gtactttgtt atttattatg ttgtcaaaag
    12481 aattttcaaa gtatctataa gaaagcttag ctcctgtcat taactctgca ccttttgcac
    12541 agtttataac ttttttagtt acttcatcta aataacttct ttcagcagct cttacgaaaa
    12601 acttacaggc tgctttttct ggaactatat ttggagcctc tcctccattt gaaactatac
    12661 catgtattct aacatctgat tttacatgtt gtcttagtgc atttatccca gcaaaagtaa
    12721 gttgtactgc atctagtgca ttgactccca tatgagggtg actagctgca tgtgctgata
    12781 ccccttcaaa actaaactct aatgaatcta ttgccaaagc cttacagttt atattattaa
    12841 aagcttctat atgcatttga taacttacat caacatcatc aaaagctcct gccttaacta
    12901 aatcacattt tcctcctgtt gtttcttcag ctggtgttcc tattagtaca atttttcctt
    12961 taaaattttc ttttaactta cttaatacta tacaagctcc tagtgtagat gctgcaatcc
    13021 agttatgtcc acaagcatga gctggttctt tattttctcc atatccagga agtgcatcat
    13081 actcagctaa aaatgctatg gtaggtccat cattatctcc taattcagct ctaaaagcag
    13141 tctccatatt acaatatgga taaactgtat caaatccata ttccttcatt ttttctacta
    13201 agtattttga tgatatgtat tcttcttccc ctaattcagg attgtcaaat ataaaattac
    13261 aaacttcttc aatttctttt ccaagttcat tctctatctc ataagctctc ttctttatac
    13321 tctctatcat aatttacctc ctactagaat cctagatata atcctattgt caaaaatgcc
    13381 attacaagaa ttgttaatac taccattaaa ggaattacga atctaaccca ctttgaccaa
    13441 ggaatattgg ctactgctag tgctcccatt aaaacacctg atgtaggtgt tatcaagtta
    13501 gttacaccta taccaaattg ataagctgta actatcattt gtcttgatac atctactaag
    13561 tcgccaagtg gtgcaagtac aggtattgta agtgatgcaa gccctgatgc tgatggtatt
    13621 aaaaatgcta ttaatccttc tattaagaaa gttaaattta taaatatagt ttttggtaat
    13681 ccatttaata aatttgctgc agaatttaaa attgtatcta ttataaatcc attttctgct
    13741 attattacga ttccacgtgc aaatgctata cacaatcctg ctgtaattaa atctcctgct
    13801 ccacttataa atgaattaac tatctcatct tgtttaagcc ctgatgctat tcctgatatt
    13861 atacctatag ctgtaaatat cattgcaatt tcagttatgt accattctag tcttaaaact
    13921 ccatatatca taatcgccat acctattgtg aaaatggcta ttaccatggc ttctcttttt
    13981 gtaaattcct ttatatttgt atcactatta accattaatt cttgatttag tgatatatcg
    14041 tgtactaaag attttttagg gtctttttta actttattgg catacatcat aacaaatcct
    14101 atagatatag ccatatatat tataaacata acaactctaa actctattcc agaacctggt
    14161 actatttgtg ataatgcttg tgctattcct acagagaaag gattcgtagt agctgctcca
    14221 aatccagctg ttgctcctat aaatactgtg gccactgctg tcaatgaatc atatcccata
    14281 ctggtcataa gtggaataaa tatcatataa aaaggtagtg tctcttcact cattccaaac
    14341 gtcgtcccac ctagtccaaa tagaatcatt gaaattggta ttattaaaaa ttgaaacttc
    14401 ttcattttat ttacagtatg agctataaca gcttctatag ctcctgtttt attaactatc
    14461 ccaaaagctc ctccaactat taaaacaaat ccaactactt cagctgcatc tataaatccg
    14521 tttataggtg ctgtaaatac atcagtaatt ccttgtggat ttgatggtgc tttttcataa
    14581 gttccagcta caacaactga ccttccgtct atatctactc tttcaaaatt tccacttggc
    14641 actatccaag taagaattgc cattaacact attagcaaaa atattattgt aagtgtctgt
    14701 ggcatagtaa actttttctt ttttaaactg ctcattttta atctccccac tttttaaatt
    14761 aaatatttta ttattgcatc cctttatcct attttgtata gatgtaaaaa agctcaacat
    14821 ggatacatgt tgagctaaat gtgctaaata caacatacct gcatcttccc tttatcttgt
    14881 ttaagatagc tctccatcaa aattcgggat aattttgatg acagtactgt acctattaaa
    14941 tacagtccca gcactagctt cggacactag cacttcggca cttactcctt ttatattgcc
    15001 tcattggttt ccaacctctt caatattaat cttagtaaat gcaacctcta cctcaatctg
    15061 tttgttagat gaggtaattc ttattaattt ttattaattc aaagtaattg tagcataata
    15121 tgtcgagatt gtaaatattt tcagtataat aattcttact ttatgatttc attccatttt
    15181 tcacaaataa ttttttctcc atataattta aatactgtag tatattttaa atacacacaa
    15241 actttccaca aattttaaat tattataata cctttataat aaaaaataaa agctatgtaa
    15301 acttatcaat ttacacagct ttatatacat taatctactc caagtacagc tttaagcttt
    15361 gatgctattg taataactcg tggaccaaat ataacttgaa ctgagttttt ctttttaaca
    15421 actccacttg cacccaattc atttacccag aagccatctt catagaatcc atcaattgaa
    15481 tttagctcta ccctaagtct agttgcacag tttgtaacat tttttatatt attacttcct
    15541 cctaaacctt ctattataga atgtacaagc tttgtatctt tatcatctac ttcctcttct
    15601 actgcattgt tgtatgctgt tttaccagca tctacagatt gtctttctct atattctttc
    15661 ttactataaa gcttaacttc ttctgagctt cttcctggtg tttttatatc aaacttcttg
    15721 attgcccatg taaatattat ataagtaact ataaaaaata ttggtatcaa aaataataat
    15781 ggtaacgcat ccactttttg tggttgtaat aaattaggaa acatatttct aaatgattct
    15841 cccattatag aaacattaaa catttctgta aatacatacg tcaatcctgc cagtggtaca
    15901 tatgctacaa aatacaataa tggtgcaaca aataggaatg tatactctat tggttccgtt
    15961 acccctacca acatggctgt tactattgct ggtatcaata tacttattgc ttttttctta
    16021 ttagcattat cagccaactt atacatagct aaagctgctg ctggtaatat tgcaaaatgt
    16081 attaataaac gacctgttgt gaagttacga gttatataag atagtgtatc tgggtctccc
    16141 atttgagcta atcttatatt attaacaccc tcgtaaattg ctccacctac ttccattgtt
    16201 ccacctactc ttgtatattc tatagggaat gcaattaagt gatgtatacc aaatggtaat
    16261 aatgctttat ccattgctcc aaaagtaaat gttcctaata atccactaga acctatccaa
    16321 ttaccaagtt tagataatgc caaccctact ggtggccata taaagtacat tgctgtaccc
    16381 aatactattg atgctataaa catcataatc attgaaaatc ttggacctga gaaaaaagat
    16441 aatgcactag gaagttctat gtcaacaaat ttattatgta ctgcactagc tacaaatcca
    16501 gctattatag ctccaaatat actacaatca tatgaaaaga ttcctaagaa agaaccatat
    16561 aaagaacttc gactatatgc ttctgcctct cctaatccag atgccataaa cgcttctgct
    16621 gtaactgtat ctgaatttat accatttgct gctaacaatg tacttataac tgtgtgcatt
    16681 cctataaata aaacaattcc tcctaatgca ccccatcctt tttccttttt agataatcca
    16741 aaggctatac ctgtaacaaa gaatattggt aagtatctca ttaccataaa tcctaaatcg
    16801 tttattactt taaagaatac atttataaat gacccttctt ttattagtcc gctaagccct
    16861 aatgcagaaa cgttttccat atttgcaaaa gctgacccta atcctataaa tactcctgct
    16921 acaacaagta atataacagg tatcatcatt gctccagaaa gtacttgtaa tttatccttc
    16981 atagtattat ttcctccatt tttattgaaa tgccaggcta tataatgcat atatatatac
    17041 ttctattgct ttgtataatg tatctatact tatattttca tttgcctgat gtgaatttgg
    17101 attatccccc tctattgtgg gtccaaatgc aactccattt tttaacactc ttgcataaga
    17161 tgcagctcct tttgtaaaaa gttcgacttc ttttccaaaa ccttgtttat atgcatttga
    17221 taatttattt attaattcac tatctggttt tacatacagt aattttaaat ccttatatat
    17281 atcaactatt aaattttctt tttttccaat ttcattaatt ctattagtta taaactccca
    17341 acttataccc ttaggatatc taaagtctat tttcatattc aattcatttt tctctaagaa
    17401 gatactcatt atacatattg tagaaactcc catatctaca tcttctttat acatccctaa
    17461 tttttttcca tgattatcat ctgtaaagta tgaatttaaa atatccctta ttataacgcc
    17521 tttatcattt agttttttaa ttttctctaa atcttttgct aaactaaatg cacagttata
    17581 tgacctgtgt ggatttctcg ataaagccct ttctcctgta tatcttacta atactttacc
    17641 ttcatctaat tcttctatct ctgtataata agctaatgat tctactaaat ctttcttatc
    17701 attagtttta aaaacagcct caattttgtc acatacaaat ccatcttctt tgtttgactt
    17761 tatatcaact aaattgtgat ttctaaattt atctttatct attttctttc ttagattgaa
    17821 gtataaaata cctttttctc catttactat tgggaagttt ccatctggag aaaatgcaaa
    17881 ttttggctgt tcattgacac taaagtaatg ctccatacat tcccaagtcg tctcttcagc
    17941 tccacccact atcaatctaa tctttctctt cggcttttca ttaagttctc ttaagaaaag
    18001 taatgcatat aaaatcccta tcaaaggtcc tttgttgtca ttaactcctc taccataaag
    18061 atagttatct ttttgacata ctttaaaagg tttactcttc caaagttctt tattatatat
    18121 tggcaccaca tctatatgat taagcacccc tactacttct tctccttctc catattctat
    18181 atgaattgca tatccatcaa agtcttttac aacaaaatct ttgtcttttg ctatttgaat
    18241 aagcttatcg aaggcatttc ttatttctat cccaaatggt tgatttatat ctgttgtact
    18301 ttcatctcta attgaaggta tttcaactag acttctaata tcatttatga tattttcttg
    18361 attggatttt aaataatcaa taattagttc tttgtttact atcatcttaa ttatttatca
    18421 cccctttata ttaattttac aaagcatttc agttcatatc tacattataa attttgaata
    18481 ttattttttc aatatttcaa atacttctct aagttaccat aatgatacgg taaacaatca
    18541 acagattatg acaaaatcat actataccta ttactaatat tttaacttat ctattacgtc
    18601 ttttttaatg taagtttatt ccaaaaaata cataaaaaaa gatgtagcct tagctacatc
    18661 ttcttatatc tattcaactt ccattattat gctattaata tctacatttc caggttctat
    18721 cctatctttt actgttctct taacagaatc agcatcatcc attataacta caggagttat
    18781 tatatttaaa tcatttgacg ttataaaatc taaatctgct tccattatta aatctttagc
    18841 ttttacctta tctcctgcct ttaccttaac atcaaacggc tttccttcta gtgacattgt
    18901 ctctaaacct acatgtatta atatatttgt attatcatta ctcttaatca ttattgcatg
    18961 ctttgtatca aatacattga ctatttcacc atcaacagga gaatataact taccttcact
    19021 tggtattatt gctactccat ctcccatcat cttttgggca aaaactgcat ctggaacatc
    19081 ttctaagttc actaaatttc ccttaaaagg gctatatatt ttctcaatct tattttcttt
    19141 tttcttaaat atattaaaca taacacactc tccacattat aaaattttat gataatattt
    19201 ttcttaaagc ttttgctaca ccatcattta cattagtatc agttacaaaa tctgccatag
    19261 attttacttc atctgtagca tttcccatag caataccaag acctgcaaat ttaatcatgg
    19321 atatatcatt ttcattatca cctatacaaa taacttcttt gctatctata cctaatatat
    19381 cacaaaattt tttaactgca ataccttttg aagtaccttt actcataact tcaaagttta
    19441 tctttccaga acttactact tctaaatctt cttgtttttt aaattcttcc tttgcttctc
    19501 tcaaagaatc tacattttct gagaaaagta tacattttaa aatttcatta tcagcttttt
    19561 ctaataaagt ttcacaactt gcattttcta ttatttcaat tctgtcttct tctttatttt
    19621 tactatttat taatctatat ggatgttctt cacctatttc attgttggat ataactattt
    19681 tacagccttt aaaatgtttg tttaaattat acttttcacc tattgtatag attttcttta
    19741 gagcttcttt gctcaaaacc ttcttatact catatcccag taatttaaaa tatgtcccat
    19801 tagttgatat aatatatata tcatccccta aaacacttgt aaaatatttc atagcattat
    19861 aaggtcttcc cgtaactaag gctaccttga tacccttttc catgacttct tttagtgctt
    19921 taatattttc ttctgatact tcaaatcctt tccccatcaa agtaccatcc atatcagtac
    19981 aaattaattt atatcgcatt tgtatcacat cctagtaaga tttttctaat atttcaacaa
    20041 ggtcttcttt ctttccaaaa ggtaaaaatc ctaaaactgg acagcttgtt atttgttcta
    20101 attgttcctt tgttacatta aactcagaca tttttgtctt aagtcccact ctctttaaaa
    20161 aactttctat ttcatttcct aaagttttag ctgcttcatc atcttccact ttttctaatt
    20221 cttggtttag cattctcgct actttggcaa atttctctat attatcctta tagaactttt
    20281 ctgcaaattt tgggaacact atagccaaag cctcaccatg aggaatatgt gtaactcctc
    20341 ctattatttc acttagtgga tgtggtgctg ctgctccagc atttgccagt gaacttcctc
    20401 ctaatgtatc tgctactgct aactttgttc tgtattctat attattttta tcttccatta
    20461 ctttaggtag atagttaatg actaattcca tagatttttc agacattatc tctgtaaaat
    20521 tattagcatt tggatttata taactttcaa atgcatgggt aaaagcatca aatgctgttg
    20581 atgcagtaat tctctctggc agagttaaca taagctctgg gtcaactata cattcttttg
    20641 agaaacaatt ttgatgaaat attgtatttt tctcgcttcc tctcgatatt actgctgctt
    20701 gtgtaacttg gcttcctgtt ccagaagtag ttgatacagc tattaatggt aattcttttt
    20761 cacttacctt atcgtatatt ttaaatggat ttgtatatgt actaaacata taatcccaat
    20821 ctattttttc taaaccaaaa gtcaatgcta atacttttgc agtatctata cttgaacctc
    20881 ctccaacagc taaaacaacg tctacagatt cttttttagc taattcaaac ccttcgttta
    20941 ttaattctac agttgggttt ggctctactc
//
LOCUS       DAEORZ010000060        20854 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEORZ010000060 DAEORZ010000000
VERSION     DAEORZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.060"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            60..881
                     /locus_tag="KRH39_002029"
     CDS             60..881
                     /locus_tag="KRH39_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBG8511931.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRYMMEEIVKVHFDKVEEHLLEQVMTAFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGITIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            902..2098
                     /locus_tag="KRH39_002030"
     CDS             902..2098
                     /locus_tag="KRH39_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG8511932.1"
                     /translation="MFIEFFYLLRSRRLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKTDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKVYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKIIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKIKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            2265..5663
                     /locus_tag="KRH39_002031"
     CDS             2265..5663
                     /locus_tag="KRH39_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG8511933.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNENINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IIGEGEHILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEVRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGLGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKLIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDVVHTPEFEEELAELGMSQSFFKISDSLGVLFIN
                     NTDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDGFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGSSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEEEEELSLELWLNRPDKADVIIVSPTGEKSKR
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINSSLWQSSSRG
                     PTIENRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNAGYGLLDVRGMFDVLR"
     gene            5673..7346
                     /locus_tag="KRH39_002032"
     CDS             5673..7346
                     /locus_tag="KRH39_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG8511934.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPIDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVDNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAISTND
                     GGLSQDNIGTGTLVSGILVGNGRVNSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSTGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEASHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIGLRDIKPGVWILRLTSELI
                     IGGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLDIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            7619..7813
                     /locus_tag="KRH39_002033"
     CDS             7619..7813
                     /locus_tag="KRH39_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBG8511935.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    7982..8215
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8292..9689
                     /gene="asnS"
                     /locus_tag="KRH39_002034"
     CDS             8292..9689
                     /gene="asnS"
                     /locus_tag="KRH39_002034"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBG8511936.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFVRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(9862..11036)
                     /locus_tag="KRH39_002035"
                     /pseudo
     CDS             complement(9862..11036)
                     /locus_tag="KRH39_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            11455..12819
                     /locus_tag="KRH39_002036"
     CDS             11455..12819
                     /locus_tag="KRH39_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG8511937.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEITSLKPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYILVLVILLVLTISDSSYLNELDMSSYSNRLNLFTILPQIASYMWAFANGISVTIIN
                     STISMFTLSSSSLFGDTKLMFYAMGALSMLIILLNGYKLRFKYNTDSIKPIIVFSIYY
                     AFLMGMLALFSTFILDSNINFFNTTSYGATLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            12841..13332
                     /locus_tag="KRH39_002037"
     CDS             12841..13332
                     /locus_tag="KRH39_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG8511938.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIVCGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            13607..14278
                     /locus_tag="KRH39_002038"
     CDS             13607..14278
                     /locus_tag="KRH39_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBG8511939.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            14363..15229
                     /locus_tag="KRH39_002039"
     CDS             14363..15229
                     /locus_tag="KRH39_002039"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBG8511940.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEVAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            15262..16764
                     /locus_tag="KRH39_002040"
     CDS             15262..16764
                     /locus_tag="KRH39_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG8511941.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            16894..17346
                     /locus_tag="KRH39_002041"
     CDS             16894..17346
                     /locus_tag="KRH39_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBG8511942.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            17506..18309
                     /locus_tag="KRH39_002042"
     CDS             17506..18309
                     /locus_tag="KRH39_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511943.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            18367..19254
                     /locus_tag="KRH39_002043"
     CDS             18367..19254
                     /locus_tag="KRH39_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG8511944.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            19273..20121
                     /locus_tag="KRH39_002044"
     CDS             19273..20121
                     /locus_tag="KRH39_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8511945.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            20184..>20854
                     /locus_tag="KRH39_002045"
     CDS             20184..>20854
                     /locus_tag="KRH39_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG8511946.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQDDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELS"
BASE COUNT         8189 a         2217 c         3743 g         6705 t
ORIGIN      
        1 aaaaatagta ataaagtcta tatacattta gaaaatactg tttaaaagga gtagaattta
       61 tgataaaaaa agagatatct aattttagag gaagtagtga ttatgtagta tcaccagaat
      121 tgatggcatc agttaatgtt gctattgctc ttgaaaagcc acttttaatt aagggtgaac
      181 ctggaacagg taaaactatg ctagcacaag caatttcaaa tgaattaaaa aaagatttag
      241 taatttggaa tataaaatca actacaaaag cacaagaagg gctttatgta tatgatactg
      301 ttcaaagact ttatgatagc caatttggag gagaaggtgt agatgatatt agcaagtaca
      361 taaaatatgg taaactagga gaggcattta gctcaaatca acaagtaatt cttttaatag
      421 atgaaataga taaggcagat ttagaatttc caaatgactt gctttgggaa ttggataaaa
      481 tggaatttta cataaatgag acaaaagaaa ctgtaagagc taaacaaaga ccaatagtta
      541 ttataacttc aaatgcggaa aaagaacttc cagatgcttt tttaagaaga tgtatatttc
      601 attatataga atttcctgat agatacatga tggaggaaat agtcaaagtg cattttgata
      661 aagtagaaga acacctattg gaacaagtaa tgactgcttt ttattggatt agaagtttaa
      721 aggatataca aaaaaagcct agtacatcag agttaataga ctggatacaa gcattaactt
      781 tgagtggaat cactattgaa aaaatagaaa aagaagttcc ttttgcagga atactactta
      841 agaataatga ggatattgaa tctatgcaaa gacacttata gttaatagga gctggataat
      901 tatgtttatt gaattttttt atttattgag gtcaagaaga ttaaatgtat ctttaaatga
      961 atggatgact ttaattgaag cattggataa aggactgtgc tactcaaact tttcaaattt
     1021 ttactatctt tgtaggatga ttttaataaa aagtgaaagt gattttgata agtttgatgc
     1081 agtgtttttg gagtatttta aaggtataga acaccaagaa gaaattccag aagagattat
     1141 gaattggctt aaaaaaacag atatagattt agaagagttt gaaagattag aacaaaatcc
     1201 aaatataaat cttgataaat taagagccaa attggaggaa cgaataaaag aacaagactc
     1261 tcctcataat ggtggtaatt attggatagg cacaggaggc acttccgagt taggacattc
     1321 aggaaaaggg caaactggta tacgtatagg tggaaattct acttatggta gagctgttct
     1381 tgaggttgca ggagaaagaa aatacagaga ttttagagat gatgaagttt taaatatgcg
     1441 tcaatttcaa gttgcactaa agagacttcg tcaattttct actagaatag atgctccaaa
     1501 aacagagctt gatttggata agactattga agagacttgt aataatgcag gatatttaaa
     1561 gttatttttt gagaagccaa gaaaaaatac agtgaagctt ttattgctta tggattcagg
     1621 tggttcaatg agagggtata gcactttatg taatacatta tttcagtctg taagtaaatc
     1681 aaatcatttt aaagatgtaa aggtatatta ttttcataac tgtatttatg atagattatt
     1741 tacaacacct gaatgttggt taagtaaatc tattaatact gaatggatat taaaaaatat
     1801 agataaaaac tataaaatta taattgttgg agatgcttct atgtcgcctt ctgagttact
     1861 tcatattggt ggtaactatc gtggtccata taattacaca cctggtatag agtggcttaa
     1921 aaggtttaag ataaagtaca gtaaaattgt atggatgaat ccagaactta gagatggatg
     1981 ggattcaatt aattattggt accaaactca aagaatgata caaagtgaat ttaatatgtt
     2041 tccactgact gtaaaaggtc ttgaaaaagc attgaaaaat ttaatggttt caagataaag
     2101 taaatataaa tgatggtatg atttattcaa aattgtacca tcattattta tttttttaat
     2161 atatctatta taaattgtat aactgaaact taaaaaatta tatagtctaa tacattttag
     2221 acttacacaa aacatataca ttaataataa ttttagaagt cgaggtgatt ataataaatt
     2281 atgaattaat tgtaaaatac aatggagata tattgagact agaagaagaa ttaggtgtat
     2341 cagtagagat acttaattca tcatatgcaa taataacatc aagtaatgaa gaagatgtaa
     2401 atacactttt aacttatcca gaaattgaat ttatagaaaa gccttttata ttacagactc
     2461 aggatgtgca aagtttttca agtacaggaa taacggggtt taaaaataga actggtttaa
     2521 ctggaaaagg aactataatt ggtataattg attcgggaat agattatact ctacctgtgt
     2581 ttagagatag tgatgggagg tctaagatat tatattattg ggaccaatct attcaaggaa
     2641 atccaccaga aggttttaga gaaggaacat tatatactaa tgaaaatata aataatgcaa
     2701 tagatggaag tatgtatata cctatttcaa caactagttt acatgggaca catgttgcag
     2761 gtatttgtgc aactattgca agtgatgcca gaataatagt ggttagagtt ggaaatatac
     2821 agaccgatat tttttcgaga agtacagagt ttatgagggc aattaaattt attttagata
     2881 gggcattaga attaagaatg cctgttacat taaatataag ttatggaagt aatgaaggtt
     2941 cacatagagg aacgtcttta tttgaacaat atatagatga tatgtgttta ttttggaaaa
     3001 acaatatagt tgtagcagct ggaaataatg cagataaagg aggtcataaa aggattaggc
     3061 ttcaaaataa tattacagaa gaagttgagt ttataattgg agaaggagag catatattaa
     3121 acataaatat atggccagat tttgttgatg actttagtgt acatttagta aacccatcaa
     3181 ataatcaaac acaagcaata tctctaactt caggtgaggt aaggaatact ttgggtgaaa
     3241 ctagaataac tggttatttt taccctatag caccgtattc tcttacaaga agagttactc
     3301 tacaattaag ttcaaataca cagataactc ctgggttatg gaaaattgta tttgaaccaa
     3361 tagatatagt aactggtaat gtaaatatat atttgccaac ttctgaagga ctaaatagaa
     3421 atactagatt tctgattcct actcaagagc ttactgtaac agttcctgga acagcaagta
     3481 gagttataac agtaggtagt tttaattcaa gaacagatat agtatctata ttttctggtg
     3541 aaggagatac acaattaggt gtctttaaac ctgatttatt agctccaggt gaagatatag
     3601 tatctttttt accaggaggt acaagtggag cattaactgg aactagcatg gctacgcctc
     3661 atgttacagg ggtttgttca ttgtttatgg aatggggaat tgtaaatgga aatgatttat
     3721 tcttgtattc acagaaatta agggcattac ttctaaaggg cgcaaggaga ttaagtaatc
     3781 aatcttatcc aaataattca tcaggacttg gttttttaaa tttatcagat atagatttgt
     3841 atactctatc taatataaat caagatttag aaacagagga tattggatat agaagtatta
     3901 ataagtcatt taaagatgag gaaaatagtt ataagcttat agatggttat aatatgcaaa
     3961 tacataatga cttagagaat gaaatttata taagtaaaaa tgcaagtaga caatcaggaa
     4021 tactatcagg aatagatgta gtccatactc ccgaatttga agaagaacta gctgaattag
     4081 gtatgagtca aagttttttt aagataagtg attcattagg tgtactattt ataaataata
     4141 cagactatag tagcatacaa cgagttttac aacttccttc aatcataaga actgtatcga
     4201 ctacaaagat gacattactt ggagaaatta atcgaggtac ttttggaggt gtagttgcaa
     4261 cagaagaaat gggcgtaaat ttctttaaaa acaatcctaa tattaatata acaggaagag
     4321 ggactctaat atcaattgca gatacaggaa tagactatct acacccagat tttatttatc
     4381 cagatggtac ttcaaaaata gtatatttat gggaccaaac aaaagaagga actccacctg
     4441 atggttttta tattgggaca gaatatacac gagaagatat aaatcgagct atcgcagaaa
     4501 atgaccctag tctatctcag gatgaagttg gtcaaggaac tatgttgagt ggtatatgtg
     4561 cagggcttgg aaatgtaaat agtgaatatg caggtatagc agaagattct gagctaataa
     4621 taataaaact cggcaagata gatggttttt ataatagtgc aatgttgttt gctgcatctc
     4681 agtatgcata taaaaaagct tttgaacttg gaagaccact agttataaat atgtctttag
     4741 gtagcagtag cttggcaggt ttaaccaata ggagcaatag tgaaaaagca ttctttacta
     4801 gagggttatg tataacagca ggagcaggaa atgagggcaa tacacagaca catacatctg
     4861 gaataatacc atatgttgga ggttctgttg aagttgagtt agaactaaat gaagaagagg
     4921 aagaattatc attagaactt tggttaaata gaccagataa agctgatgta ataatagtat
     4981 ctcccactgg tgaaaaaagt aaacgtgttg gaatttcaaa ttataataaa gtaacaggtt
     5041 tatttgattt agaagggact gaatattcta taacgtatat atacccaaca actttttctg
     5101 gacagcaatt tactaatgtc actttaaaaa atgcaaaaag gggtgtatgg aagattcgat
     5161 tagtaggagt ttatattata acaggaagat ataacttata ccttccaaat agagaacttt
     5221 taaaaagtgg aactagattt agagaagtag acccttttta cactataaat tatcctgcta
     5281 ttcaagatga tttaataact ataggagcct ataatactat taatagtagt ttatggcaaa
     5341 gttcatcaag aggaccaact attgaaaaca ggttaaaacc agatatagta gcaccaggtg
     5401 ttaatataat agctgcttat cctgggaaca cgtatgcaac tataactggt acagcagcag
     5461 caagtgcaca tgcagctggt gctgccgcaa tgtattttca atatactttt gttgatggta
     5521 gatatccaaa ccaagcatat gtgcaaaaaa taaaaacgtt tatgcaagct ggtgctagaa
     5581 aagattcaaa cacagtatat ccaaatacaa atgctggata tgggcttttg gatgttagag
     5641 gaatgtttga tgttttaaga taggagggat ttatggaaaa atcttattgt ataatttatc
     5701 aaggtgatat agaatctgcc ctacaagaaa atggaataaa taggtatatg gttttaaata
     5761 gtcagcttgc agtaatatat gtacctatag attttgatga aactatttta aataatataa
     5821 tacaagttgc ttggtgggaa gaatctgaac caatgagtag tctaattgaa ataactaata
     5881 atgtagacaa tggggaaacc attacaacag cagctgaaac tgattatatt tatgaaaatc
     5941 catataatga tataacagga agaggaattt tattagcagt tatagattct ggtatagatt
     6001 atttgcatcc tgattttata aatgatgatg gaactagtaa agttttatat ttatgggatc
     6061 aagaagcaaa tacaaatcca cctccagaag gttttatttt tggaagtgaa tttacaagaa
     6121 gtgagttaaa tatagccata agtacaaatg atggaggttt gagtcaagat aatataggta
     6181 ctggaacttt agtttctggt atattagttg gaaatggaag ggtaaattcg cagtatagag
     6241 gtattactac agaatcagac ttaatagtag ttaaattaaa atcatataca gatacttatt
     6301 atgctggaag gataaattac agtgtttcag attttttagc agctataacg tatgttacta
     6361 atatagcaag aacagaaaat aaaccactga taataaattt gactattgga gttaagtcaa
     6421 gtgccgtagc aactacttca atactagata cttttaatat attgagtagt actggtgtgg
     6481 tagtagtgag tggtgcagga aatcaaggga atacagatat ccattattct ggcagattta
     6541 gcagtgtagg agaagttcaa gatgtaataa tacaagatgg agatgattat gcacttgata
     6601 taacactaaa tacgaatggt ccagataaag taggagcaca gataatatct ccttcaggtg
     6661 aagctagtca tgatataagg tattctccag acttttatat atatagagga aaatttaact
     6721 tagaaaatac tacttatgca atgagattta tttatccata tattacttct ggaaaagaaa
     6781 atctggagat aggattaaga gatataaaac ctggagtatg gattttaaga ttgacttcag
     6841 aacttataat aggtggagaa tatgacatat acttgccaaa taaaaatttg atagctccag
     6901 atacaaggtt tttagaccct gattcagtag ctacaataac tatgtatgct gctagtgatg
     6961 atgtaattac agttggtaca tttaataata agactgatag tatgtggatt ggttcttcga
     7021 aagggccaat tagaggaaga ggaattaagc cagatatagt agcttcaggt gtagatataa
     7081 tatccacata taaaaatgga acatataata caggaacagg aacaggagtg agcagttcta
     7141 tagtcacagg tgttttagcc ttattgatgg aatacttaga aaaacaggat aatgtaccga
     7201 gattatcatt gtttacacaa gttttaaaaa catatttaat attgggtgct acaaaacttg
     7261 atatatatac atatccaaat gtatcgcagg gatatgggat tttaaattta aaaaatacaa
     7321 ttcaacaaat agcaaatact ctatagataa gaatctatgt aaaattataa ttgattaata
     7381 gttcaatttt atcaaatatt ctaggtagag taaaaaatgg cataaaaaat aaatatgaaa
     7441 attaaaaaaa cagttttata ttttgaatac acaaagtaaa tattttgtat ataaataggt
     7501 ataaattaag tattgaaaag atatgatgct aatttaaaaa tcattataat ttcgacttat
     7561 gaattttatt tggaaaattg ctaaaactat tacaaaataa ataatagagg tgatagtttt
     7621 ggaaagtaaa aaggcaagag cattaaaagg taaagataat aaaagactta aaagttcact
     7681 tttagttgga aatgagtcaa cagcagcatg ggctgatgtt gaaaaattaa aacctcatag
     7741 taaagtttca ataccctcaa ttaagggtgt ggaagaagca aaagactggg ttgacaatgg
     7801 aagtaaatta taaaattttg gaaattgaag taataaagaa gtaataaaga taagtattta
     7861 ttgtaattat gcaagctaga attattatct tttaaatagc aatcagtatc aattatataa
     7921 atttattgac taaaaattta taaattggta taattttgtt aattagcaga aataataaaa
     7981 gataaatact gtgaagagaa gagtagatat aaaatgtagt tctagcgagt tgaggatggt
     8041 gaaagctcaa tacgaggttt atattgaaga acaccttgga gtttctaacc gaaagcttat
     8101 ttagtaagta gacttagacg gaaggcaccg ttataggctt taagcattgc ttaactaaga
     8161 ggtcattatg acaactaggg tggtaccgcg agagtaatag tctttcgtcc ctttatatag
     8221 ggatgaaggg ctttttttat tgaaattata tttaaaagaa attaataaca attatactag
     8281 gaggatttaa aatgcaaaaa gaatttataa cagtaaaaga attgtacaga gaaaaagaac
     8341 agtacatagg gaagactgta aaagtagctg gatggataag aacatctaga gtatctaaaa
     8401 attttggatt tatagagcta aatgatggaa gcttctttaa aaatatgcag atagtatttg
     8461 atgagaaagt agaaaatttt aaagaggtag gaaaattagc aataagctca tctatacttg
     8521 tagagggaga gttagtatct acagaagggg ctaaacaacc agtcgaaata catgcaaaaa
     8581 gcatagttgt agaaggggaa tcagatagtt cgtatccact acaaaagaag agacatacgc
     8641 ttgaatattt aagaacgata gcacatttaa gaccaagaag taatactttt tcagctgtat
     8701 ttagagtaag atcaatagct gcctatgcaa tacacaaatt tttccaagaa agaaactttg
     8761 tatatgcaca ttctccaatc ataacaggta gtgactgtga aggtgcagga gaaatgttta
     8821 gaataacaac tatggattta aatgatattc caaaaacaga agatggcaaa atagactata
     8881 ctcaagattt ctttggaaaa gaggctaatt taacagttag tggtcaatta aatgccgaga
     8941 taatggcact ttcttttaga aacgtttata cttttggacc tacatttaga gctgaaaact
     9001 cttatacagg aagacatgct tcagaatttt ggatgataga gccagaaata gtttttgcag
     9061 accttgaaga taacatggaa cttgcagaag atatgataaa atatgttata agctatgttt
     9121 tagaaaatgc tcctgaagaa atggaatttt ttaacagttt tatagataaa ggattgttag
     9181 aaagattaaa taagatagta aactctgagt ttgttagaat aacatatact aaagcagtgg
     9241 aattattaat tgaatctggt catgaatttg agtatcctgt tgagtgggga tgtgaccttc
     9301 aaactgagca tgaaagatat ataacagagc aaatatataa ttgtccagtg tttgttacag
     9361 attatccaaa agatataaaa gcgttctata tgagaatgaa tgaagatggt aaaactgtta
     9421 gagcaatgga cttgttagtt cctggagttg gagaaatagt aggaggttct caaagagaag
     9481 aaagagaaga tgtattgtta gatagaatta atgaaatggg attaagagaa gaagattatt
     9541 ggtggtattt agaacttaga aagtttggta ctgctactca ctctggattt ggtcttggat
     9601 ttgaaagaat aattatgtat atgacaggta tgtctaacat aagagacgtt atacctttcc
     9661 caagaactcc taaaaatgct gaattttaat aattatatat aataagactt atataacttt
     9721 aagtataaac actaaaatga cttgcacatt tatctatttt agagatatgc aagttatttt
     9781 tattgtacta taaatatata atttgattac ttttattttg cgatataata cttataggtt
     9841 tatgtaatag tatgtaactt tttaataaaa tgcaacacaa ttacgtccct gttcttttgc
     9901 agtatataga gccttatcac aattacttat aaactcattt aatgttattg tcttttcaat
     9961 tttctttgta taaactccaa tactaacagt tacacatttt aaaggaacat catcacgctc
    10021 aaaattgcag gactctataa tttttcgtaa acattctgat ttaagtttag catctgtctc
    10081 atcacagtta aaaatacata taaaaaattc cttaccacca tatcgtatag catgttcatt
    10141 ttcagaactt gtaaaatcta aaattacttt ggcaatattt tgtagacaat agtctccctt
    10201 ttgatgtcca taattatcat taaattgttt gaaataatct atatctatca tcattccagt
    10261 tataggttct tgatttgcat ggctgattaa ttttttttgt agaatatctt caaggtatct
    10321 acggttgttc attttagtaa ggtcatctgt cataactttg tcgttaagta aattatttat
    10381 atgagatatt tcttcgtatt gttgactaat taaaagtttg tcatagtaag taaatacacg
    10441 atttctatat agcattgtag aaacaaaaat ggcaagggat gttataaaaa atgtgttgac
    10501 aatattacta aactcattgc ctggtatagt ttgaatccca ggtaagaata aattaagaaa
    10561 caaaaagcat cctccaaaaa ctattactga ttctccaaat tttaatactg agaaagctgc
    10621 agctgctaac ataatgtaac taaatacact taggtcattt ccacctaact ggtcaagtag
    10681 agtaattaca caaccccata ggcagaaaaa agttacataa attatccata aatttatata
    10741 aaacgtacaa caatcctttt tttattccaa agataagaaa atacaaacat aaagacagaa
    10801 gatacacaaa atagtgttac atacatataa aaataccatt ggcgtcttgg tgtggcaaaa
    10861 gggcctcctt ctctagctga gattgatatc atcataatta tttgagatat tactattatt
    10921 atgcaaatga ttttacccgt atgataaaga taattgcaga gttctttttc aacattttct
    10981 ttgtgttttt tgtctagagg aaatccaaaa aattttttta ttttttctaa gttcatataa
    11041 gcctccatta aaaataaagc ataaaaataa gtattccttt ctgtccttag atacttggaa
    11101 tacttcgttt ataatttaat tatatcaaaa tatattgtgt gtaacaatct aaaattgtga
    11161 tagataaaat ttattagcta atgttgaata actttattga ttaatacttt attataatta
    11221 agatttacag atagtttgct actgaaaata aagaatagaa taaatttaaa ttaagcataa
    11281 taaaatataa ataataaagt tcgcattaac aaactattta aatatcaaga cttgttaaaa
    11341 aacaacagca ataaacaaat aaaatagata aatatatcca attaaggtat aattaagttt
    11401 tatatgatat agttatattt gataatttac aatagtaaaa cttggaggga aaaaatgggt
    11461 gaaaagaaaa taaataatat gaattattca cataataatt attccaaaaa gacaggtgaa
    11521 ccaactagaa aaataaaaaa tagccatata actcttaaaa gtaaagaaga aaaaatttgt
    11581 tctagttgca ataaagaaaa taatactaag gataattact gtaaattttg tggaaatgaa
    11641 ctttatgaaa taacatcatt aaaaccactt gaaacaaaac tagacttgaa atctaaaata
    11701 aaagagcttt catatcatgc aaataaaaga ggtgtatttt tgacaacatt tacaaccata
    11761 tttatattat ttattattgc attgatattt aaagctatta taactattca atttaatgat
    11821 attagttatt taataaatcc agcacatata atcctagctc tcaacttggg tcaaattagt
    11881 gtatctatgt ccaccatgat gggttctggg tttataaatg ccaatatagg tttgttgata
    11941 ttacttataa tccctatttt ggtattatta atatcaaacc taatttttat gagaaaaaga
    12001 tgtagagatt caaaaactgt attagcaaat tctctaggag ttggaatttt ttatggatta
    12061 gttttagcta ttttatctat ttttactaat gtaaaaacta gttcacatag tatgttagaa
    12121 tatggatatg cattagaata tagttatgaa ttttttagtg ttcttttaaa tggatttgta
    12181 ttagggttta tatgtacata cataactact tataaaaaaa gttatgaaaa ggaaaatatg
    12241 tatttatctc ttttttcaaa ttctataaga acatttatat taggttatat tttagtatta
    12301 gtaatactat tggtactaac tatatctgat agtagttact taaatgaact agatatgagt
    12361 agttattcaa atagattaaa tttatttact atactgccac aaatagctag ttatatgtgg
    12421 gcttttgcaa atggaatttc agtaaccata ataaattcaa caatatcaat gtttacttta
    12481 agttcttcat ctttatttgg agatactaaa ttaatgtttt atgcaatggg agcattgtct
    12541 atgttaataa tattattaaa tggatacaag ctaagattta agtataatac agatagtata
    12601 aaacctataa tagtatttag tatatattat gcatttttga tgggaatgtt ggctttattt
    12661 agcacattta tactagatag taatattaat ttctttaata caactagtta tggagcaact
    12721 ttaataatgc aatttaaagt cttacaagct attgtaattt catttgtata tagttttgtg
    12781 atatctttaa taggatataa gctaaactca gctgattaaa tatgattgga gggaacttct
    12841 gtggaaggaa atactaataa taaagtagat gataaatttt ctaaggttaa gaaaaagttt
    12901 ggtagtggaa aatttaaagt tgaattatat aaagaaaaat caaaattaaa tagagagatt
    12961 gatgatattc aagaaaagaa agcaaagata ttcttggaaa tgggaatact gacatatcaa
    13021 aaaattagag aagattctat taatgataat agttttgata aattctgtga tgagttacta
    13081 gaacttgata agttaattta tgaaaaaaat atggaaatta atgaaatgga aatggcagaa
    13141 agtaatgtaa cttgtgaatg tggttatgtt ggaaatgtaa atgataaata ttgtgctgaa
    13201 tgtggtaaaa aatttgaatt agaaaatgaa tgtgatgatt ttatagtttg tggttattgt
    13261 gaaagtgaga tagatacaga ggctgaattt tgtccatgct gtggaagaaa aattataatg
    13321 gatttagaat aatattggga aacctatgta tagtatacat gggttttttt attgaaatta
    13381 tttaaatgga ctgaatataa tagtggttat ataaaatgaa aataaacacc aaaaaaatga
    13441 aataaaattt cttttaagta taaaaaacaa gctaatatat agaataaatc agtaaaaata
    13501 tagagttgag aaaaaatatg aaaaaatatt gcaaagaaaa agttttcatg ttattattaa
    13561 tacataaatc aagaaaataa atttcagaaa tgaggtaatt aaaaaaatgc tagataaggt
    13621 aaagggaaga ttaatagtat catgccaagc tttagaaaat gaacctcttc atagtccttt
    13681 tataatgggg agaatggcta aggctgctat ggaaggtgga gctgttggaa ttagagctca
    13741 aggagttgaa gatattattg agataaaaaa agttactgga cttcctgtta taggtattat
    13801 aaagagaaac tatgaagatt cagatatata tataacgcca actaaaaaag aagttgatga
    13861 acttttaaca acagggtgtg aaatgatagc tttagatgct actaatagag ttagaccaaa
    13921 taatgaagac ttaaaagaac ttataaagta tataaaagaa aacggtgttc tagtaatggc
    13981 agatatttca aattatgatg aggctatcaa agcacaagag tacggtgtag attgcgtttc
    14041 aactacacta tccggatata ctccatatac taaaacatta gaaggcccag attttgtatt
    14101 aatggaaaga ttagttaaag atttagaaat acctgtaata gcagaaggaa aggtaaatac
    14161 tcctcaagat ttaaaaaagg tttttgaact aggagttcat tcatcagttg taggttcagc
    14221 aataacaaga ccacaattaa taacagaaaa atttgttaaa gcaattgaaa ttaattaaaa
    14281 attaatttat aaattaaaaa attattatat ttaactatac ttgaaaatta aaattggagg
    14341 taaagatatg agaacaacag atatgaaggg aatttattca gcattattag tttcatttga
    14401 taaggaagga aatataaatg aaaaagggtt aagacaaata attagacata atatagatgt
    14461 ttgtaaagta gatggtcttt atgtaggagg aagtacaggg gaaaacttca tgctttcaac
    14521 tgatgagaag aaaagaatat ttgaagtggc taaagatgaa gtgaaagaag aaataaaatt
    14581 aatagctcaa gttggttcag ttaatttaaa agaagctgta gagcttgcta aatttacaac
    14641 tgatttagga tatgatgcaa taagtgctgt tacacctttt tactataaat ttgattttga
    14701 agaaataaaa cactattata atacaataat aaattctgta gataatagat taataatata
    14761 ctctatacca tttttaactg gtgttgatat gagtttagac cagtttggag aattatttga
    14821 aaatgaaaaa ataataggtg ttaagtttac agcagcagat ttctacttat tagagagaat
    14881 gagaaaaaca ttcccaaata aattaatatt tgcaggattt gatgaaatga tgttaccagc
    14941 tacagtttta ggtgtagatg gggcaattgg ttctacattc aatgtaaacg gtgtcagagc
    15001 tagacaaata ttcgaattaa ctaagaatga aaaaatatca gaagcacttg aagtacaaca
    15061 tgtgactaat gatttaataa cagatatatt aggaaatggt ttatatcaaa caattaaatt
    15121 actacttgaa gaacaaggtg ttgaggctgg atactgtaga caacctatga aagaagcaac
    15181 tgatgaaatg aaatcaagag caaaagaaat atacagaaag tatttttaat aaaatacttt
    15241 tagtatggga ggattagtca aatggtagga tttacttgga tagattttgc aatactagtg
    15301 gtttacttat tggcagtttt atttgcagga ttgttatttt caaaaaagga aatgaaggga
    15361 aaagagttct tcaaaggtga tggaactatt ccgtggtggg ttacatcagt atcaatattt
    15421 gccacattat taagtccaat atcattttta tcattagcag gaaattctta tggtggtact
    15481 tggattttgt ggtttgcaca attaggtatg tttatagcaa tacctttgac aataaaattt
    15541 ttcttacctc tatatagtag gttagaaata gatacagcat atgaatactt agaaatgaga
    15601 tacgaaagca aaggtcttag agtacttgga gctttgatgt ttataatata tcaaatagga
    15661 cgtatgtcta tcataatgta tcttccatca atagttctgg ctggtcttac aggaatacct
    15721 gtaaatgtac tgataatagt tatgggtgta atagcaataa tttactcata tacaggtgga
    15781 ttaaaggctg tcttgtggac cgattttata caaggaatgg tattaatagt aggtgttaca
    15841 gggacattgt tctatttaat agcaagtata aatggtggtt ttggaacagt aatggatact
    15901 cttacaactg gacacaagtt cttagcaagt aatgaagtta tgtttgataa aaatatacta
    15961 tctacaagtg catttataat atttgttgga gctggtctta atactttttc atcatatgta
    16021 tcgagtcaag atatagttca aaggtttact acaacaactg atataaagca acttaataaa
    16081 atgacatatg gaaatggtgt tttatcaatg ggacttgcaa cagtatttta tctaattgga
    16141 acttgtttat ttatatacta tactcaaaac ccagatttag ctcaaacagt tcaacaagac
    16201 caagtatttg catcatatat agcttatgaa ttaccagtgg gtataacagg aatactactt
    16261 gcagcgatat atgcagcttc tcaatcaact ttatcgacag ggcttaattc agttgctaca
    16321 agttggactt tagatataca aactatcata acaaaagata taagtatgga aaagcaaact
    16381 aagatagcac aatatatatc attaggtgta ggtatacttt ctatagctgt tgcaatagta
    16441 ttagcaaatg gagaaataaa gtcagcatat gaatggttta atagttttat ggggctagta
    16501 ctaggtgttt tagcaggaat atttgtactt ggtgcatttt gtaaaaaagc gactaaaatg
    16561 ggagcatatg ttggattcat agtatcagca attttagttg tttatttaaa ataccgtatg
    16621 ccagaagtaa cttcttgggc atattctcta ataacaatca cgacttctgt agtggtaggt
    16681 acaatagtta gtgcaataga agctaaagtt aaagcaaaag tatctttacc aaaagctgag
    16741 actactgttt attatgaaaa ataatattta aatgataaat tgtcttatag agtgattaga
    16801 taaataattt agttactcta taagattgta taatatactt tgtatataaa agtataaaaa
    16861 tagactttat aattatatag ggagatagtg cttatgatat ttggaaatat aaatcatagt
    16921 aagacatatt caacattgca tgaagattta cttagatgtt ttgaatatgc taaagataat
    16981 tcattaatag attatgaaaa aggtacttat acaatagatg gtgatgatat ttttgtgaat
    17041 attgtagagt ataaaacttg tgaaaaagaa gatagattct gggaagctca taagaagtat
    17101 atagatgttc atttgatgct tgatggatgt gaacgaatag atataaattt tatagaaaat
    17161 ttagaacaaa aaatatatga agaaaagggc gatttcttgc ctcttgatgg taaaaataat
    17221 ggatacatgg aacttagaaa aggtgatttt ttagtttgtt atccggaaga tgctcatatg
    17281 acaggaataa aagttttaga aaaagaaaat atcaaaaaag ctatttttaa ggttttggtt
    17341 agataatgat aatttatata accactaggg ctgaaaatag tgatttaact ttcagcgatt
    17401 agtgagactt cgtaaatttt agttagatta ctattttcag ccttagtttt ttatgagaaa
    17461 attgaaaata attaaaagct taaaattttg taaaggagtt aacttatgga aaatatagat
    17521 gtattcttat taataggaca aagcaatgct agaggattgg gaaatccaaa agagagcatc
    17581 attccaaatg aaaattgttt tgaatatttg agtactgatg aaattataaa tatgagatgt
    17641 gagctagaga cttcagaagg tgatggtact atagcacctg cattttcaaa tgaatggaat
    17701 aagcttactg gaaataaagt ttgttttatt cacaatgcaa aagatggttc aagaataaaa
    17761 aactggaatc atgataataa ctggtttcta aatgacacta tagaaaaatt taatacaggc
    17821 tgtactacct taagtaaaca ttataatata gaaaataaat acgttatttg gattcaaggt
    17881 gaaagtgatg caaaatatgg tagtgatgta ttgtactata aagaaagtct aaaaaaaatt
    17941 gcgtataggc ttaaagaaga atgtatgatt gataaaatgt ttgtaagttt aactggttat
    18001 tggcttggag aagatgaata ttttattaga acaagacgaa ttgcagcagc acaggagtcg
    18061 gcttgtagtg aatgtgatat attatgtgta ggaagcaaaa ttgctatgca gtttcatgac
    18121 agaggattaa ctattgatga tgttcattac actcaagaag gtcttaatat gttaggagag
    18181 gacttatgta aaaatattta taagtatcat ataaccaaag aaaaaagtgt attaagtgat
    18241 actatagact tatcagaagc aaggaaatat atctgtgaat tggaaaagat aagtaaaaga
    18301 tttgtgtaat ataaagtttt aaatagagtt gttaatttat aaaaataatt tgtatggaga
    18361 tatgttatga aaaattacgt ttgtatagat gttggaggta cttcaataaa atatggtttt
    18421 attagagaag atggatttat aattgataaa tcaagtcttg atactgaagc aaaggaaaaa
    18481 ggtggagaag gaatattagc caaaataaaa gatatagtaa aaaaatatat agaggaaaat
    18541 gaaataagtg gtatatgtat atcaactgca ggtatggttg acccagttga aggtaaaata
    18601 ctatttgcat tagaagaatt aattccaaac tataagggca tgcaattaaa aaaagaagta
    18661 gaaaaagagt ttaatataaa atgtgaagtt gaaaatgatg ttaattgtgc tggacttggt
    18721 gaaatgtggc ttggagcagg tagaggtgca acatcttcta tatgtttaac aataggtact
    18781 ggaattggtg gatgtattat aataaataat aaactgataa atgggtttag taatagtgct
    18841 ggtgaagtag gatatatgaa tgtaaatgga agttcttttc aagaattggc atctactagc
    18901 agtctaatta aaaaagtagc aaaaataaaa aatttgaatg aaaatgactt aaatggaaaa
    18961 ataatttttg atttagcaaa gaataatgat gaagattgtt taaaagaact cgataatatg
    19021 ataaaatcat tagcagttgg aatcgcaaat atatgttatg taataaatcc agaagtggta
    19081 attttaggtg gtgggattat ggcacaagaa aagttcctaa aaccaaaaat agacaaagca
    19141 ttaaaggagg ttcttataga gagagtttac aaaaatacta atatagaatt tgcaaagaga
    19201 caaaatgatg caggaatgct tggagcatta tataattttt taaacaaaat ataaagaata
    19261 gaagggtttt taatgagtat tttagaacaa ttggaatcac caacttttaa agtcacaaaa
    19321 tcagataaat tattaatagc ttatataaaa gaaaatatag atgatgtatt ttataaacca
    19381 atatcacaaa tagctaaaga gagcaatatt ggtgaagcaa ctataactag gtttgtaaaa
    19441 aaaatgaatt ttaatggatt acaggatttt aaagttactt tagcacaaga aatatcaact
    19501 atcaataaaa aaaatattat aaataaaaat atacaaaatg atgagccagc tttggataca
    19561 gcaaaaaaac ttttaagttc aaatgtaacc actttagaga acacagtaga gataatcaat
    19621 agtaaggatg tacatgactg cgcaaggctt ataataaatg caaagaaagt ttacttcata
    19681 ggtattggat attctggtat aatagcacag gattcaaact ataaatttat gagaataggt
    19741 cttaattgtg taagttttga cagtagtcat acaatgataa tgatgtcttc cataatggaa
    19801 gaaggagatt taataattgc aatttcacat tctggtgaaa cagaggagat tataaaaact
    19861 gtaaagcttg caagagccaa taatgctaaa attatatcaa ttactgaaaa taaaaattct
    19921 gaattaaagg atatttcaga tgtacactta tcttatgtat cgggagaaac agtactggaa
    19981 accggttcaa tatcttctaa attagcacaa tttttcatta ttgatttagt atatacacag
    20041 gtagtaaaag aactttctaa tgaagctatt gaaaggaaaa ttaaaactac aaatgctata
    20101 aaattgttta aaaatgaata aaataagaaa tttgtaatgt tttatagagc tctcttaaaa
    20161 atttaggaat ggagagatat tacatgtcta aattaaaaga ttataatatt gataaagtaa
    20221 taatttttaa taatttaagt actaaaacga agaatcaact taaagaaaaa gttaaactaa
    20281 aaagattaca aaaaaaagaa attttatttt atgaaaaaga tagcttagac agtgtttata
    20341 tacttttaga aggaaaggta tctattttta agataagtga aaatggagag agaaaagtaa
    20401 ttttcatatt aaatagtggg gaagtcataa atgatattac acttgacgct tctaaaaact
    20461 ctactgttgg ctgtgaagca tttgaaaatt caattgtagc ttattatagt attgaagaat
    20521 ttttagaaat tatgcaagat gattttcaac ttacaaagga tattatttct tatatggaaa
    20581 gaagaataag acgtttatat agacagttga aaaattctat atctataaaa gtagataaaa
    20641 aacttgcagc aaaattatat aggttaagta gagaatttgg agtttgctgt ggagaatgga
    20701 ctttactaaa tgtaaatatt acagttacat atttagctga tatgcttggc tgtaaaaggg
    20761 agactgtttc cagaatgata aaggtattgc aaaaagaaga acttattaaa attgacaata
    20821 aaggttttta tgttaaagaa actgaattat caag
//
LOCUS       DAEORZ010000061        20846 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEORZ010000061 DAEORZ010000000
VERSION     DAEORZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20846
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.061"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(736..1125)
                     /locus_tag="KRH39_002046"
     CDS             complement(736..1125)
                     /locus_tag="KRH39_002046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511947.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLERDAKGY
                     KIVNKNGFFNEFDFYEAVNICLNISIEDAIKSYNILVKSLVMLDKRLGKRRLKNIYED
                     IEKENNLVKKFYNIRVESENMILKKNA"
     gene            1221..1870
                     /locus_tag="KRH39_002047"
                     /pseudo
     CDS             1221..1870
                     /locus_tag="KRH39_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
     gene            2412..3767
                     /locus_tag="KRH39_002048"
     CDS             2412..3767
                     /locus_tag="KRH39_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8511948.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLYSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(3885..4553)
                     /locus_tag="KRH39_002049"
     CDS             complement(3885..4553)
                     /locus_tag="KRH39_002049"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBG8511949.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREELVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(4620..5048)
                     /locus_tag="KRH39_002050"
     CDS             complement(4620..5048)
                     /locus_tag="KRH39_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG8511950.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            5155..5748
                     /locus_tag="KRH39_002051"
     CDS             5155..5748
                     /locus_tag="KRH39_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBG8511951.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            5868..6803
                     /locus_tag="KRH39_002052"
     CDS             5868..6803
                     /locus_tag="KRH39_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBG8511952.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHIIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(7074..8018)
                     /locus_tag="KRH39_002053"
     CDS             complement(7074..8018)
                     /locus_tag="KRH39_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG8511953.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKRIMYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKICYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            8336..9766
                     /locus_tag="KRH39_002054"
     CDS             8336..9766
                     /locus_tag="KRH39_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8511954.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            9966..10550
                     /locus_tag="KRH39_002055"
     CDS             9966..10550
                     /locus_tag="KRH39_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBG8511955.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(10654..11433)
                     /locus_tag="KRH39_002056"
     CDS             complement(10654..11433)
                     /locus_tag="KRH39_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG8511956.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEI
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(11491..12747)
                     /locus_tag="KRH39_002057"
     CDS             complement(11491..12747)
                     /locus_tag="KRH39_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBG8511957.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPDGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            13005..13904
                     /locus_tag="KRH39_002058"
     CDS             13005..13904
                     /locus_tag="KRH39_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBG8511958.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSEFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            13928..15376
                     /gene="ddlR"
                     /locus_tag="KRH39_002059"
     CDS             13928..15376
                     /gene="ddlR"
                     /locus_tag="KRH39_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBG8511959.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            15435..16241
                     /locus_tag="KRH39_002060"
     CDS             15435..16241
                     /locus_tag="KRH39_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG8511960.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDESISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            16267..18186
                     /locus_tag="KRH39_002061"
     CDS             16267..18186
                     /locus_tag="KRH39_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG8511961.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(18428..18775)
                     /locus_tag="KRH39_002062"
     CDS             complement(18428..18775)
                     /locus_tag="KRH39_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBG8511962.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(18762..20690)
                     /locus_tag="KRH39_002063"
     CDS             complement(18762..20690)
                     /locus_tag="KRH39_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBG8511963.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
BASE COUNT         7751 a         2986 c         2709 g         7400 t
ORIGIN      
        1 aaaaaaaaat aaagattagt attaaaatgg taattatagg tatatttgct gtaattattg
       61 cttttgtatt atccagatta ttcgttgagt atattgatga attattaagg tctaattggc
      121 aatatcagct atttgaaagt gagagttcat atatagtatt tgccaattgt attatgttct
      181 cgaatatatt attagaaata tttttaatat atatttgtag aaaatttaga aaaatctaat
      241 atggtcaacg tatttcgaat cgaatacttc atgattattt agtctattgt gctagttaaa
      301 gtttaattcc catataaagt aaattgattt tagtaaaaca aatcacccct aagggttagt
      361 ttatggataa gtcatccaaa tattaactct ttaatttttg ctatagattc atctttgctc
      421 ataactttac ttataaaaaa tgaaatatta taagctagaa cttttattct aattctagtc
      481 ataaatccta gcatagagtt acttttttta tattataaat aaaactatac aacaaagata
      541 atatttagta aaaataactt tcattttaac taaacttaaa attcaattac tctctaaatt
      601 gacgtttagg aattagttca aaataaaaaa gagtgtatat attgaaatta ctacatttca
      661 atatatatac tctttttaaa tttctcagtt ttccatggaa ctttacttta tttatttctg
      721 tattagttgt attttttatg catttttctt taaaatcata ttttctgatt caactcttat
      781 attataaaat ttctttacta aattattctc tttttctata tcttcatata tattttttaa
      841 tcgcctttta cctaatcttt tatctaacat tactaatgat ttaactaata tattatatga
      901 ttttatcgca tcttctattg agatatttaa acatatattt actgcttcat aaaaatcaaa
      961 ttcattaaaa aatccatttt tatttacaat cttatatccc tttgcatctc tttctaaata
     1021 ctcttcaaaa ctttcatcat ctaatctaaa tcctatatca ctatgaaaaa taggttttgc
     1081 atttggatat agttctattg ttttatcaaa agtttcgaat accaagttat tattattcga
     1141 ttttccaata gtccactata aacaataata agtttgttgc tccaaataat gtaacacttg
     1201 tagaaagatg ttacagatat ataaaagata gagaatgtag tttttctcct aatactatta
     1261 gaaatagaaa aaatatgatt aaaaatcaaa ttgaagtttt ctttaaagat atgaaattaa
     1321 tagatataac tccttctatt ttacagtctt atataaataa catatacaat gagcacatgt
     1381 taaattcaac aaaaaaccaa gtcgatttta taaagtcagt attgaaagaa tcttatagat
     1441 taaaagaaat atcagaaaat atttgtaatt ttgttacaac cccaatcaaa aaaaatagtt
     1501 caacttctaa gctttataca aaacaaaaag ctcaattatt attagaaaaa tcaaaaaata
     1561 ttcctatagg tataccagtt tttttgatgc taactcttgg attacgtttt ggtgaagctg
     1621 tatcacttat atggtcagat gtagaccttg ataaaaaatc tcacatgtaa atcaaacatt
     1681 agtttatgta tacaataaaa taatcttcaa agaccctaaa acccctatat ccaaaagaaa
     1741 attatttgct tctgatgaac ttataagttt gcttaaaggg gaaaaattaa aacaaaataa
     1801 gttaaaatta cagggtatat tgaaaaatga atttgatttg atatgcttaa ataaaagatt
     1861 caacccgtga gttcaaccaa cttttgttgt tcattttaaa aaactattaa gagataataa
     1921 tcctagagat ataaaagtac atgaattaat acatgcaaat gcgactttga tattactttc
     1981 aggtactaac ataaaagcta tttataaaat attagtacat gcagatataa aaatagctat
     2041 gaatatatat tccatattct cacttattag atgaaatgaa taaagaaaca tctgaaaata
     2101 taagcaaaat actattcaaa taatgttcag ttttatgtca attaaatgtc actcaaagtg
     2161 ctttttttcc atttgtcagt taaatgtcac ttaaagtacc tttttcctat ttgtcagtta
     2221 aatgtcagta atatataaat aagatatatt tataagaccc ttaaaatcac tattatttaa
     2281 taaaatttat aattatatac tatttgttgt tgacattggt attataccat agtttatcat
     2341 ttatattaga aataaaatta ttaattaata gtttcgtaat tttataatag atttacaggg
     2401 agtgataata aatggataca aaaatatcta taaacgaaaa catgagtcta ggtaaaagat
     2461 tttttaaata cctagcacca tctgtagttg caatgtgggt tttctcacta tatacaatgg
     2521 ttgatggaat atttgtaagt aaaggagttg gagaattagc tttagctgct gtcaatatat
     2581 caatgccttt tattaatttt atatttgcag tttctctatt atattcaact ggtgcatcta
     2641 ccataattgc aatctatctg gggaaaaaag atattaaatc tgctaacgaa gtcttttcat
     2701 ttaacttagt atcaataatt atattatcaa taattatatt agctataact ttttttaatt
     2761 tagatagatt agcactattt ttaggagcta cagaatctac aattggtatg gttaaagatt
     2821 atttaggaat aataatattt tttaacggat tctttatagt gtcttattct ttagaagtta
     2881 taataaaaac tgatggcttt ccaatattag ctactgttgg tgtaataata tctgctctta
     2941 caaacatcat acttgattac ttgtttgtaa ttgaatttgg atggggtgta aaaggtgctg
     3001 gtattgcaac tggtttgtct caagtctttt ctactatttt cttcttaata cattttttaa
     3061 gaaaaaactc tactttgaat ttttcaaaat ttagaattga ttttaagaca cttagaaaaa
     3121 ttgtatttat aggttttcct gattcaacta ctgaattaag ttgtggtata gttgttttac
     3181 tttttaactt aagtcttact aaatatattg gagaaaatgc tcttatttat tatagtgtca
     3241 taaattatat aaatacatta gtacttatga ctatgatggg tataactcaa ggtatgcaac
     3301 cacttactag cttttattat ggtgcaggaa atatagataa tgtaaaaaaa ttattaaaaa
     3361 tggggataaa agccacaata atagcatcag ttgctgtatt tgcaatttgt atggcttttt
     3421 caggaccaat agtctcttta tttatacatc cagaagaaac aatgctattc aatgaaggag
     3481 ttcgtgtatt taaaatattc tctatatctt tcttattggt aggaattaat gtaataattt
     3541 ctggattctt tgtatcagta gaaaaaccat caatttctac tgttatatct cttggtagag
     3601 ggcttgtaat agttgtttta agtctaattt caatgatact aatatttggt ggacaaggaa
     3661 tatggatgac tactattgtt tctgagttta tatgtcttat tttatcatta gtttttctaa
     3721 aaaagaattt ttctacatta gactctaatc taaataaggt tgcttagatg ataagagtga
     3781 caatatatga aaatatctaa ataaaaaata cctattatac aatcttgcaa atattaaacc
     3841 tttgcaaaaa ttaggattat ataataggta ttttatttat gtaattattt tatttctaaa
     3901 tatccatagc catcttttat taaagttcct tttacttcat attctacagg ttttttaaat
     3961 attggaccat ctacgacaaa tgtatgatac tctccatgtt ctccacatgg gtctgcacca
     4021 gcatttttta tcttctccac aagttctcta gttaattttt ttcctaaaaa ttcctctcct
     4081 aagtgcttta agtttacttt tgttacaaca ctacaaaatc cactgtctat aaattcatat
     4141 actaactcct ctctactctc ttgccataat ggtaaatcta ctttcatacc aactgcttca
     4201 catctatcag tatcccattt tctatgactt tctatatcta tatctccata tgcacatata
     4261 gttgctccca tgtttttagc ttttattaaa gccctctcaa agctctcttc atatccttct
     4321 acaccacact cagcaactaa taaaggtaat cctatattgc tacttacttg cttaagcata
     4381 ttatattcta aattatgtgt ccatgacttt ccatgttctt cacttatagt tgtcaagagt
     4441 gccacaggct tatatccttt tttcaacatt cgatttaatg ctaatatact atccttacca
     4501 ccactaaatg acattacaaa tttttcatca gccaatttta acacctcttt catctaactt
     4561 tgttttatta acataattat accaaatttt tgagttgcat agcaaattat attaaattgt
     4621 taattcacta taaagaatgt ccctaatatt aaaagtataa cccctatgta tttgtataaa
     4681 tgcattgttt cttgtcccaa aattacgcta tcaataaaaa atccaactaa aacttgggat
     4741 acaagagtta atataagtgt atttgatact cctaaatttg gaattgcctt tactgtaaaa
     4801 tatattatta gacctccaaa tactcctcct aataacaact tataattaaa accatttata
     4861 gatactagac actttaagtc acctgtaact attatacttg ccaaaaaaaa tattgctcct
     4921 acagttaagc ttataaaggt ggccactaat gctgttgtac tttttcctag ctctccattt
     4981 ataaaagctt caagagctgt agaaccacct gctaataatg caaatattat tgcccataat
     5041 tttgtcatgg acaaacctcc ttctatataa ttctattaat agaagtttat gatacaacct
     5101 attaattaat tactaagtat agtttaattt ttatacacta ggctttattt ggatatgaat
     5161 ttctgttata attgttctat cgattttgga ggtgatttta tgtacacaaa ttttaatatc
     5221 gattttaata actttaataa aaaagaaaat gctattaaat ttatgttaat gggagtatta
     5281 ctcattatat taggattatt atgccttaca tttaagacat taggtataaa attaatttct
     5341 tggacttttg gtattgcact tttattcttt gcatatttaa atttaaaaaa tattaatgaa
     5401 ctaaaaagat atgctactaa agaagaaatt aaaccctcta ttaatatcca atgggttcta
     5461 ataattgcat gtatattact ttttgtattt cctcaaaaaa tacaaagcat attttcatta
     5521 tttttaggat tttatttaat ctttaatcaa ctagtagcac ttgtaaatag taagaataat
     5581 ccttattcta aatttacaac ttggaatata gttaagattc tatttggtat atgccttata
     5641 ttatctccac tttttttatc tagatttata gtatcgataa tgtctttttt cataatatta
     5701 tttggactag ttctattttt ctctggaaat acagcgagaa aatattaatt attttctcgc
     5761 tgtattttta tattacttaa aatagaattt gtttaagtta tctatttgtg gtaatatgtt
     5821 ttttgtacaa gctcagcgta caattttatt agaaataggt gatttttttg gataaattta
     5881 aatatgcttt tgataataaa agatatcata cttggaatta ctatcttaga aatacttttg
     5941 gagaaaaagt ttttaaggta tctataaatg caggatttac ttgcccaaac attgatggaa
     6001 gtcttggtta tggcggatgt acttattgta gcaaagaagg ctcaggtgat tttgctggaa
     6061 accctaaaga taatcttatc agtcaatttt ataatataaa agaaatgatg ttaaaaaaat
     6121 ggcctcatgc aaagtatatt ggatactttc aagcctatac aaatacgtat gctcctcttg
     6181 aagttttaaa agaaaaatat gagactatat tagaacttga agatgtcata ggtctttcaa
     6241 tatccacaag acctgactgt ttgccagatg atgttgtaga atacttgtct gaactaaata
     6301 aaagaaccaa tttatgggtt gaacttgggc ttcaaacaat tcatgattct acatctaaaa
     6361 ttataaatag aggtcatgat tataaaacat ttgttgaagg tgttgaaaaa ttaaagtcta
     6421 aaaatataaa ggttgtcgta cacattataa atggtcttcc tggtgaagat tataatatga
     6481 tgatggaaac agcaaaggca gttggtaaaa tgggtgtaga cggtataaaa atccatctat
     6541 tgcatattat aaaagatact cctatggaaa agatgttaca aaatggtatg ttaactctta
     6601 tggaacaaga tgaatatatc aatcttgtct gtgaccaatt agaaatactt cctgaaacta
     6661 tgataatcca tagactcact ggtgatggta agagggatga actagtaggt cctatatgga
     6721 gcctaaaaaa atgggaaatt ttaaatcaaa ttgatgatac tttaaaggct agaaattctt
     6781 atcagggctg taagtttgtt taatacaaaa taataactat agaaatggtt atataaatat
     6841 agtcctttct atagttattt agtatttaca aaacaaaata ttaatttgta catttataca
     6901 atatataata aaaaatagat agtctaaact gtatctactt atattttatt tgtactgtcc
     6961 actttattct ataaaattta taaatagtta ttttttaata ccaaatataa tttggtataa
     7021 tttttgttgg actctaaaat gtataccaat aaatcctttt taagtatata attctaaata
     7081 ctttcagttt tttcaatctg ctttaacttg ttggacttat aaaatattct tatttcatca
     7141 taagaaagat taagtgctac tactgcagat attaacatca acatcactcc aaataacttc
     7201 cctaaggaaa aactttctcc taatatagtg caaccaatta ttactgaacc aactaattct
     7261 actgatgcca caacacctgc aactgacaac tctacacctt tagatatacc tgctgcataa
     7321 aaaatatatg caagtgcagc tggaacaatg cctaacatta gcatacaaga taaaacatct
     7381 aaatccttag cataatttaa gatttcccaa ggtcttgaag aaggaatcat aaatatagct
     7441 ccaaataaga aactgtatac taatattgtt gaacttgaaa attcctttaa agcattttta
     7501 ctaatagttg gcataagtgc atatgctatt gctgataata ccccaagtaa aagtccaaaa
     7561 gcgtttaaac cttgaaaatc caaacgtcca ccagtcactg ccataattgc accaataaaa
     7621 caaagaataa gtgaaaataa tttgcttcta gtaatatttt ctttataaca tatttttgaa
     7681 aatattgcta aaaacagtgg tgatgtatat agtaaaactg ctgcaattga tacacccgca
     7741 atgtcaattg ctttaaaata acataaatta aacattgcct gacatataat acctattatt
     7801 actgaataca taattctttt tttgcttatc tttaagattt gtggtgtcct tatagatgaa
     7861 tatacgccta aaactataaa tccaaaaaac aatcttgtaa atgctacttg ttctgatgtc
     7921 agtccatttc ccattaaaaa ctttccaaat aatccaagtg tagcccataa taaccctgct
     7981 attgctataa atacataccc tttacttttt tctcccattt taaattccct tctattttta
     8041 tttagtcaat ttacactctt ctttttaagc aaaaactatg ccatcttaaa tcaatatata
     8101 tcgactctaa aaccctagtc tttccaataa tactaataaa ttttcaattt ttttatattt
     8161 atttttcgct tactttaagt tgatttagac ttattttaat tgtaaaataa gttatatata
     8221 acttatttaa agttaaaata gactgaaact tatatataaa tatcaaattt taagtatatt
     8281 aatcaattaa acctatttta atatatcatt aaaaatttag gaggaaaatg taaaaatgaa
     8341 tttagatttg gaattttatc aaaaaatatt agaagcttcc catgatgaaa tatgtgtttc
     8401 tgatgataaa ggaataataa tatattgtaa taaagcattt gaagaaaatt atggtctaaa
     8461 aaaagaagat attttaggaa aaaatgtttc ttttttagaa gactctggat attcaacaaa
     8521 aagccccata ccaattgtat taaaaacaaa gactaaattt tcactggaac aagatactca
     8581 aacagggaaa aagcttatta taactgctac ccctatattt gatgaaaatg gaaacttaga
     8641 atttactgtt gaaaattgta gagatataac tgaacttaat aatataaaaa ataaacttga
     8701 agacacaaaa aaacaagtaa aaaaatataa atctgaagta gaaacactat acagaactgc
     8761 acttagaatt gaggatactg taattatgga cggaatagtt atgagaccaa tcataaacac
     8821 agtaaatcat gtttctaaaa cggatgtaag tgtactttta ttgggtgaat ctggaacagg
     8881 aaaaagctca cttgctagat atatacacca caacagcaat agatctaatg gcccatttat
     8941 aacaataaat tgtgcaacta tatctccaca acttcttgaa tctgaacttt ttggatatac
     9001 ttcaggtgct tttacaggtg caagtactaa aggtaaagtt gggctcgtag aattagctaa
     9061 tggaggaact ctttttttag atgaaatagg agatatacca caaaatctac aggcaaaatt
     9121 tttgcaatta atacaggata gaacttttac tccagttggg agcttaaaaa ataaaaaggt
     9181 cgatataaga attatatctg ctacaaatgt tgatttagtt tcaaaagtaa aagaaaaaaa
     9241 gtttagagaa gatttatact atagattaaa tgttatagaa ataaaattgc ctcctcttag
     9301 agaaagaagg gataatctag tggaaattat aaaatattat tttaataggt actcaagtga
     9361 cttcaaccta aataaaacta tttctaaaga agctatggat gctattgcaa attatagatt
     9421 cccaggcaat ataagagaat tacaaaatat aattcaaaaa atcttgctta cttgtacaga
     9481 taatcatata acaatagatg acttaccaaa cattcttaca aaaaatatac atattaccaa
     9541 taatggaaat aaaacacata tatcacaaat taataaggtt attatatctg actcaaaatc
     9601 tactaattat aaaaataaaa attttgacac tttaattaaa gagtatgaaa aaaatataat
     9661 tttggatgct tatgaaaaat ttggtagttc ttataaagta gccaaacacc ttgaaatctc
     9721 acagtctaaa gccaataggt taattagaaa gtatactaat acttaatttt aaatttgata
     9781 aataaaaact taacactatt tacatattgt aaatttggcc atttactgtt attatgaata
     9841 tggttgaaat tatttaaata aatactgttg acttagtttc atatatttac cattaaactt
     9901 ttatcataaa tatcatatca taataatata tttaataagt ctaataaact atacgaggtg
     9961 taaatatgtt aatgaatacc cattttatta tggcaaaatc aatacttgat aatatagatg
    10021 aaaataaaac attctttata agcgaaaaaa attttatata tggcaatata aagcctgatg
    10081 ctttttctaa atataaatta aaaaaacatt atttagatga gtcatttaat atgattgttt
    10141 ctaaaataaa ctatctatgc agtcttaata tagatagtct ttctaaatac ttttcagtaa
    10201 gtagacttag tcaggaacta ggagtcatat gtcatttttt atgtgatttt ttctgtgtag
    10261 ctcacagtga acgttgggag tttaagcata gcttaaataa acatgttact tatgaaagag
    10321 aacttggagc atttgcaaaa gatgttgatt tatctaaaat taaatgtggt actataaata
    10381 gtagttttga gaagtttttt actaaacttt atagtgatta caaaaagaaa actgattata
    10441 tgaatgattt aaaattttca gcttatactt gtaatagtgt cataaattat atacttgact
    10501 ctattttatc taatacaata aaatcatatc atatagcaaa ttgcggttaa cttaagaatt
    10561 tttaaactta tataaactta taattgaata taattaaaat gactgctcta tagaatataa
    10621 ttttatactt ctctaggaca gtcatttttt atactaacta tttatctcca ttataacatc
    10681 tatggcactt tttacactat ctactacata atttgcactt tgctttacat cttcccttgc
    10741 cttagacatt gcaaaactat atttaaatcc ttctaacatg caaatatcat ttggagaatc
    10801 cccaatagta gcaacttcat ctggacttac atttaacgtt tttattacat gctctatact
    10861 tcctcttttt gacacacttt taggaactat atttaaagta tatgttccag aaaaatatat
    10921 ttctaatact tcactaaact cactttttag ataaatttct attctggata actcttccaa
    10981 attttcagaa catacgccta tatttcctat aagattatca aaactacttc ttttttcaaa
    11041 atctgggtca actataaagt cactagaata atgtctaaaa gcagtggtat cactacccac
    11101 aattacatca cccttatatt taaaataaag ttgtgcatca cttgactcaa atctatcaat
    11161 gacttttttt actaaattat catctatcgt tcttttaaaa attacattat aatctttatc
    11221 atatataaat gaaccatttt cacatacata atatccattc atatctaaac tatctttgat
    11281 aatattatgt gcttgtttaa aaattctacc tgtagcaaca ttaaactgta ctcccttttt
    11341 ttctatttct tcaattgcta ctatatcttc ttttgttatg gttccatttt catatagagt
    11401 tccatctaaa tcacaaaata tatgttttat cataaattaa aagcctccaa aaacaatttt
    11461 atattatact aatactttaa tcaaaataac ttaaagcttt tctaattgta ttctcaattc
    11521 attaagcaaa tctctagtag ctcttaatct tatctttctt ctatccccat taaatatatt
    11581 tttctttact attgtttttc catttatata tattgcagta taaaccagtc ccacaggctt
    11641 ctctttggtt cctccatcag gtccagctac tccagttgta gatataccta tattagcttt
    11701 aagtccttta gcaacgccct cagccatttc tatagctgtt tgctcactta ctgctccaaa
    11761 ctcttccaaa gtttctcttt ttactcctaa tcttttcatt ttagaatcat tagaatatgt
    11821 tacacaacct tcaagaaata cagaagatat tcctggatag tttataagag atgaagaaac
    11881 caaacctcca gtacaagatt cagccactgc tattgttaga tttttttcta caagtagctt
    11941 agaaacagca tcttctaaag cagtctcccc ttcagatatg gaaatatctc cttcagtata
    12001 tacatattta ccaactctac tttttatttt ttcttctact ggttttatta atttaaaagc
    12061 ttctctttca ttttcagcct ttgcagttat tcttattgta acttctaatt cttttgcata
    12121 taaagcaact gttggattag tctgctcatt aattatatcg agaatttcta attctagatt
    12181 tgactcacct attccatata atcttaaagt cttagatact agcatttcat ttgtgaattg
    12241 ttgtaagtaa ggtttgacac tctcattaaa catagctttc atttctcttg gaggtccagg
    12301 caatactatt ataaatttac catctttttt taaaattgcc cctggagctg taccattatt
    12361 attttttagt actatagcat cttttggaaa gtacacttgt ttcatattat tttcggccat
    12421 ttttttccct gttttaacaa agaaagattc caaaacctct agtgaaggtt tatgtagttc
    12481 taatttctga ccaaagtatt ctgcagcagt ttcttttgtc atatcatcac cagttggccc
    12541 aagaccacct gtagtaatta caaaatcact tctctttaag ctttcatcaa aacattctaa
    12601 aagtctttgt ttgttatctc caacagtact ctgatggtat acttctattc ccaatgatgc
    12661 aagttcttta gctaaaaatt gagaatttgt atttactata tctcccaata atatttctgt
    12721 tcctacactt attatttcag ctttcaaaat ttatcactcc ttgatttata gccattaaga
    12781 ctagtcttaa gttcataaca ataattttag caaaaataat aacataaata aagttaaaaa
    12841 atcatttaaa catatcttta aactagatta ttattctaaa accacacata atataaactt
    12901 cgtatatagt ggtactttca tcctcaactg ttaattcata taattaagta tgtaaataaa
    12961 aagtttactt taaaataata aaaattaaaa taggagtgta taatatgaaa atagcagtta
    13021 taatgggtgg aatatcatca gagagagaag tttctttaaa ttcaggaaaa gagatatata
    13081 ataaccttga taaaaataaa tatgaagttg taaaagtaat aatagatgat aaaaaagata
    13141 tatttacaaa gataccagaa gatatagatt ttgcaatact tgctttacat ggaaaatttg
    13201 gtgaagatgg atgtatacaa tcaatacttg agactatgga tataccatac tctggatgtg
    13261 gacctctttg cagtgggatg tgtatggata aaaatataac taaaaaaatg cttagagatt
    13321 caaacctacc tactgctcca tgggttttag taaaatcagt tgatgaaata gactatgatg
    13381 aaatagataa tataggttat ccagtattta taaaaccaaa ctcaggaggt tcaagtgtag
    13441 ccacattctt tattcattct aaggatgaag ttgaagaagc agttagaaaa ggtctggaag
    13501 ttgatgaatt tgtaatgatt gaaaaatata taccaggtgg agaatatact tcattcatat
    13561 taaatggaga agtgttccct acaatatcta taaaatctga ttcagaattc tttgattatg
    13621 aagctaaata ctcagttgaa aaaggtgcaa aggaagaagt tgtttattta gatgaagaac
    13681 ttcaaaaaag agtaaacgaa atttctgaga cttgttggaa aatttttaat tgcaaagcat
    13741 atgtaagagt tgatatgatt ataagtgaag gtattccata tgttcttgaa ctaaatactc
    13801 ttcctggaat gactcaaact agtttaattc ctagaagtgc tgctgctaga ggtattaagt
    13861 acagtgaact attagataaa cttatagaat actctttaaa ttagatgaaa taatttgagg
    13921 tggtaatttg atattttcaa atctaaaatt aaatgataat gaaccaattt atatacaact
    13981 taaaaattat ataagtgata tgatatcaaa aggactaatt ccagacaata gtaagctccc
    14041 ttctacaagg gagcttagtc agcttttaca agttagccga aattctgtag ttttaactta
    14101 tgaagaatta aaatcagaag gattaatcta ctctatttca ggaaaaggta catttgttaa
    14161 atcaaagaat aagtcttcaa atactacatg gtcattaaac tgggattgtt tagagaatac
    14221 atactctaaa aaagccaatg aattggacat tataaaaagt gaaattcctt ggagctctga
    14281 cctgatttcc tttaaaagta tttctccaga tggagattta ttcgacatgg aagaattaaa
    14341 aaaatccttt ttaaatagaa tttcactaga aggacataaa ttactcaact atggatatgc
    14401 acaaggttat aagccactta ttgactattt actagaatat atgacaaaca agggtgctga
    14461 tataagtaat aaagatatac taataacaaa tggattcaca gaaggctttg atattattat
    14521 gtcatctttt acccaagaaa aagactacat aatatgtgaa aatcctactc ataatactgc
    14581 tataaaaatt atgaaatctc ataacctcaa tatcttaggt gtagatataa atgaagatgg
    14641 tttggacttc aatatgctag aagacaattt agttaagtat aaaaataaaa taaagtttgc
    14701 atacattaca ccctcttatc acaatcctac aggaattgta atgactcctg aaaataggta
    14761 caaattctat aatcttatga aaaaatacaa tatagcaatt atagaagatg gatttaatga
    14821 agaattactc tacaatagtt ctcatatatt tcctatctgt tccctagata atatgaataa
    14881 tggggtcgta tatattggta gtttttcaaa aattttattt cctggaatga gaattggatg
    14941 gatatttgca gataaaaaag taatcaataa gctagaaagt gtaaaaaggt gcagaaacat
    15001 acatgtatcc tttttagacc aaggaatttt atttgattat ttaagtaatg gaggttttga
    15061 aaaatacatt aaaaaaatac gtaaattcta tggagacaaa tttaactttg catataagtg
    15121 tataaaaaaa tatataaaaa cagaatatat cttgggtgat ggtggtttgc atatatttat
    15181 aaaactaaaa aatatagata ctagagtttt attagaaaaa tgttatgaaa aaaatgttat
    15241 atttatgcct ggagacttat tttttacaga taactctggt tttgatactt taaggctcgg
    15301 attttctagg ttgtcttttg aagacattga aattggaata aaaataattg gagaaactat
    15361 aaatgaaatg ttctaggtta actaaatatt gttagcctag tttattttta tgaggtataa
    15421 tatatatagt ttatatgaat ctaatactgg gggataatat gagtaaaaag tcaaaacgaa
    15481 gaaaaattaa taaattaaaa ttaacgattg taataactgt attatttctt gctttattat
    15541 ctatatatga gatactgttc tctaatccca acaaaaaagg taatataaag agtcaagaaa
    15601 ctatgcaaaa taaaaataat acaacagatg attctaaaaa taatcaaagc tctaccaaaa
    15661 agcaggaaga taataatatc aagcctactt tttcatattc ttcaatacca gataatataa
    15721 aaaataaaat gattggtaaa tctatgccta ctgatgaatc tataagcttt gatagtctat
    15781 cttatttaaa acttacatat tatggatttg atgaaaaaac tcatcaagga gaaatgatag
    15841 taaacagtga actagctcca gaagtagtgg atatttttaa ggaattatac gaaaaaaaat
    15901 atccaattga aaaaattaag ttaatagatg actatgatgc tgtagatgaa aaatctatgt
    15961 ctgataataa tacctcatca ttttgttata gaactatagc tggtacaaat gtagtttcaa
    16021 atcatggtaa aggacgtgct atagatataa acccattaca gaatcctcaa gtttctggaa
    16081 atgatgttac acctaaagta agtacagttt atgctgatag gtctagcaca aaatttggaa
    16141 tgataaaaaa aggtgatgat tgttataatg cttttgtaag cagaggttgg agttggggtg
    16201 gctattggaa aaacccagat tatcaacatt ttgaaaaata atagattaga aataaaggtg
    16261 aattatatga atttaatgac attggaaaat ataagtaaaa gctattctga aaaaaaatta
    16321 ctagaaaata tatctcttgg aataaatgaa ggggaaaaaa taggacttat tggtgtaaat
    16381 ggtactggta aatctacctt actcaaaatt attgcaggag ttgaagaaag tgagactggt
    16441 aatataacaa aaactaatgg tattaggata gaatatctac ctcaaaatcc aacttacaat
    16501 gaaaattcaa ctgtacttga acaagtattt aatggaactt ctgaagaatt aaagctactt
    16561 ggtgaatatc aagaaatcct agaaaaactt aattcaaact ttacagagga attaaataaa
    16621 aaattattat cagtacacga aaaaatagat gccttaaatc tttgggattt agaaagtgaa
    16681 gctaagtcag tacttacaaa gcttggtata aatgacttta atcaaaaaat agcagaactt
    16741 tctggtgggc aaagaaagag agtctctctg gcatcagccc ttattacacc ttgtgagttt
    16801 ttgatactag atgagcctac aaaccatcta gacaatgata ctatagattg gcttgaggaa
    16861 tatcttaatt ccagaaaagg ttcattgctt atgataactc atgatagata ctttttagac
    16921 cgtgttacaa atagaattat tgaactggac aagggtagat tattcagtta tgatgggaat
    16981 tattcaatat ttttggaaaa gaaaatggaa agactttcgc tagaagcaag tattgaagat
    17041 aaaagacaaa atttaataag aacagagctt gcgtgggtaa gacgaggagc tcaggctcgt
    17101 agcactaaac aaaaagctcg tttacaaaga tttgatgaac ttataaatag agattctttt
    17161 aaacctgatg aaaatctcga tatatctgtt gcatcaagta gacttggaaa taaaataata
    17221 gaaatacaca atatatctaa atcatttgaa gaaaatactg ttatagataa tttagaatac
    17281 actttaactc gtacagatag aattggtata ataggcaaaa atggtatggg taaatctacc
    17341 cttataaata tacttaatgg agaaatagag cctgatagtg gatatatttc aattggagaa
    17401 acggttaaaa taggttgttt ttctcaagac gactctcata tgaatattga tatgagggct
    17461 atagactatg taaaagaagc tagtgactat atagaaactt cagatggtac taggattaca
    17521 gcttctcaaa tgtgtgaaag attccttttt aatggtacta tgcaatatac tttgataggt
    17581 aaattatctg gtggtgaaag aagaagacta catcttctta aaatccttat gacagctcct
    17641 aatgtgcttc ttttagatga acccacaaat gatttagata ttgaaacgct taaaatatta
    17701 gaagaatacc tagacgaatt taaaggtgta gtagttacag tatcacatga tagatatttc
    17761 ttagatagaa tttgtaataa aatatttgca tatgaaggta atggaaaaat tcatatctat
    17821 acaggtaatt acagtgatta tcttatttat agagagattc aaggaattga atttgaagaa
    17881 tctaaaaagg aatcagttaa aaaagatgac tctactaccc taaaaaaaga aaaacctaaa
    17941 aatgataaaa agcttaagtt ctcatataat gaacaaaggg agtttgaaaa tattgactct
    18001 gatatagaaa aactcgaatc taaaattgca acacttgatg aaagtactgc aaaattttca
    18061 actgatttta ctaaactcca agaaatttta gatgagaaat ctaaactaga aaaagaactt
    18121 gaatacaaat atgaaagatg ggaatattta aataatctag ctgatgaaat agctaataat
    18181 aagtgatatt aaaataatct taagtttaat aagttttgct ttaaatctgt aatttataaa
    18241 tattttaaag gaagtgtctc aaaatgaact ttttagtttt atgagacact cttttttatg
    18301 agaaatcaaa tatattttca tcatttcaac aatacaaaag aggcttatct ctattttgag
    18361 acagcctctt tcataattac cttctaattt atatgctcta atatatctat aataattctt
    18421 attatatcta tatatccagt ctttcaatat agtgacaatt aacttcaata tgatttaaaa
    18481 atactaacaa cctttcataa agtggatgag attcttctcc atatttagct tctaggttct
    18541 tgcctatttc tctaacagtc ctttgaccat caatttgtaa aaaaacaaaa cttgaatatt
    18601 catctagttc tatttttttg tatttaggta ttctagcttt taactttcta aaaaactttt
    18661 gaattttatg gtcctgtttt tcaagtatag taacaacact attttcagct acttcatatt
    18721 caagtccatc agtaatttta aatacaatat ctaaaacatc attactcttg ctcattattt
    18781 tttactttag ataaaacaat aggtaatgct gtaagcacaa ctaaaactac taataaagca
    18841 attgccattg aatttgttgc agcaaatcca cttggtacct ttggagttac aactccagtt
    18901 acttgtaata ttatacctat taaaccaatg atagaaccac cagcaacaag accagaagat
    18961 aaacttatac cattagaaac tttaacttct ttttcttttt cagttttaga tactaattca
    19021 acaaataaac gaattaatgc tccaactaat attatagaag ttgttgcaat tggtaaatag
    19081 aatcctatag ctatagtcat tattggtaaa tttaatgcat atagaactat tgccataaat
    19141 actccaacaa taatcataac ccaaggtaaa ctacctgaca taataccaga agttaatgtt
    19201 gacattaagt ttgcttgtgg taatgcaaat tgtacattat caccagttgc tctaagttgg
    19261 tcagaaagta ataaaattac tcctacaaca actataacac caactatact tgcaattgta
    19321 aagtaacgtt gcatttcgtt tttgctacca ccaataatat aagttacttt ttgagattga
    19381 gtataacctc cagctatagc aatagcaaca actataaatg aaccaaataa aagtaatgat
    19441 ttattagctt ctaaatcagt ccatcccatt ataacaaaca ctaatgttac aataactaga
    19501 gaagctatag tcataccaga aactggaaga tttgatgttc cgattgttcc tgttaaacga
    19561 ccagctacta tgacaaataa taaagataat aaaagagata tgatagctcc aataattgcc
    19621 ataacaatgt taccagaaat taagaatgct gctaaaaatc caataactac tccacctaat
    19681 aaaagtatca tttgtataga tgaaccttct tctccttctc cagcacttga tttagcttta
    19741 agtgtttctt ttatagaagc gattatagtt ggtataagtt ttattgcacc aataatacct
    19801 ccacaaagca tcataccagc acctatatat tttacataac tactagatat tgctccaaca
    19861 tccatttggt taagaggcat tgcaggatta ttccatacca tagctccatc tttagccata
    19921 tctgtaaagt atcctattaa aggtgctata ccaaagtttg ataaaatact tcctgcaaac
    19981 attgttaaag aaacttcaag ccctactata aatccaattc ctaaaagtag aggatttact
    20041 tcaatatcaa atttccattt gtagaatttg cttcctacaa gactcattac attgtttgct
    20101 acatttaaga atgaaccagt taatacagta ataaatccac ttattacaaa tccaattccc
    20161 atgtacttaa ttgaatctcc accagcctct gaagcaacaa gtgtttcaga tatagccatt
    20221 gattctgggt acataagttt accatgttct tcaactatta gatagttgtg aactatagca
    20281 gcaattccta taccaaataa aactccacca actccaacag ctaagccttc aaagaatgta
    20341 atttggcttc caattaatat aactgctggt aatacgaaaa taaatccact tgcaacagat
    20401 tctccaccac ttgacatacc ttggattaag tttttaccaa gaataccctt tgaacgagca
    20461 aatgctccaa caaaagcaga acctattata gctcctggaa tacctgcggc aactgtaaga
    20521 ccggctttca ttccagagta agttgtagat gcggcaaaaa tggcagctaa aataataccg
    20581 ataatcagta cagctacatt tccaccggtt ctggacttat ctgtgatata tggaacgtaa
    20641 tcttttccac taacctcacc ataagcaccc ttaggtaatt ttttgttcat aaaattttca
    20701 ccccttttca atttgtatat attataacat atatacatat aaaacttaac tttttattat
    20761 tttacaaata gcgagtcaaa actactaaaa tttaacccta tattattaat ttctactttt
    20821 agatattttt atgctaattt tctttt
//
LOCUS       DAEORZ010000062        20843 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEORZ010000062 DAEORZ010000000
VERSION     DAEORZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20843
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.062"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            322..3861
                     /gene="nifJ"
                     /locus_tag="KRH39_002064"
     CDS             322..3861
                     /gene="nifJ"
                     /locus_tag="KRH39_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBG8511964.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(4253..5179)
                     /locus_tag="KRH39_002065"
     CDS             complement(4253..5179)
                     /locus_tag="KRH39_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBG8511965.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPNNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(5674..7008)
                     /locus_tag="KRH39_002066"
     CDS             complement(5674..7008)
                     /locus_tag="KRH39_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG8511966.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            complement(7070..7846)
                     /locus_tag="KRH39_002067"
     CDS             complement(7070..7846)
                     /locus_tag="KRH39_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG8511967.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            complement(7951..8616)
                     /locus_tag="KRH39_002068"
     CDS             complement(7951..8616)
                     /locus_tag="KRH39_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBG8511968.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            complement(8616..9272)
                     /locus_tag="KRH39_002069"
     CDS             complement(8616..9272)
                     /locus_tag="KRH39_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBG8511969.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            complement(9281..10459)
                     /locus_tag="KRH39_002070"
     CDS             complement(9281..10459)
                     /locus_tag="KRH39_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG8511970.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGNITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLSKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            10814..11716
                     /locus_tag="KRH39_002071"
     CDS             10814..11716
                     /locus_tag="KRH39_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8511971.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            complement(11932..12744)
                     /locus_tag="KRH39_002072"
     CDS             complement(11932..12744)
                     /locus_tag="KRH39_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8511972.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(12747..13706)
                     /locus_tag="KRH39_002073"
     CDS             complement(12747..13706)
                     /locus_tag="KRH39_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8511973.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            complement(13812..15371)
                     /locus_tag="KRH39_002074"
     CDS             complement(13812..15371)
                     /locus_tag="KRH39_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8511974.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSNGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            15590..16567
                     /locus_tag="KRH39_002075"
     CDS             15590..16567
                     /locus_tag="KRH39_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8511975.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            16568..17539
                     /locus_tag="KRH39_002076"
     CDS             16568..17539
                     /locus_tag="KRH39_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBG8511976.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     QTLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            17691..19733
                     /locus_tag="KRH39_002077"
     CDS             17691..19733
                     /locus_tag="KRH39_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBG8511977.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQKGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSVFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            19994..20836
                     /locus_tag="KRH39_002078"
     CDS             19994..20836
                     /locus_tag="KRH39_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG8511978.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
BASE COUNT         7453 a         2924 c         3264 g         7202 t
ORIGIN      
        1 agataattat tttacttttt gtactaatat aaacaaatag ataaaaaagt ttgaaaagtc
       61 taacttaatt tattatatat tgtatacaaa attatgaaaa aaacttaaca tatttaattt
      121 taaaaaaaat ctacttaaat atgtaattaa tttacaattt tcaaaggaat tttatctaat
      181 ctgtagtatt atattagaag atagagtatt tgcgcgacta tatgtgtgtt tatagttttg
      241 ttgtgtaaat tatatgtaaa tataaaaaaa tagacttgtt ataaacaagt acaattgtag
      301 ctatcaagga ggatacatat tatggctaag tttatgaaaa cacttgatgg aaatacagct
      361 gcagctcatg ttgcctatgc gtttacagat gtagcagcta tatatccaat cacaccatct
      421 tcaactatgg ctgaagtagt tgatgagtgg gcatcacaag gaagaaaaaa tatatttgga
      481 caaacagtta atgttgttga aatgcagtca gaagcaggtg catcaggaac attccacggt
      541 tctttacaag gaggagcttt aacttctact tttacagcat ctcaaggttt attattaatg
      601 atacctaaca tgtataaagt tgcaggagag ttattaccag gagtattcca tgtaagtgct
      661 cgtgctttag catctcaagc gttatcaata tttggggatc accaagacgt tatggctgct
      721 agacaaactg gatgtgtatt attagcttct ggttcagttc aagaagtagc tgatatagca
      781 cctgttgcac atttagctgc aatagaaggt agattaccat ttatacattt ctttgatgga
      841 ttcagaactt cacatgaaat ccaaaaagtt gaattactag aaaatgaaga ttatgcaagt
      901 ttattaaact ttgaagcagt tcaagcattt agagataatg ctttatctcc aaatcatcca
      961 gttgctcgtg gtactgctca aaacccagat atatatttcc aaactagaga agcttctaat
     1021 aaatactatc aaaatatagt aggtatagtt gaaaaataca tggaaaaaat gagtaattta
     1081 acaggtagaa aacatagttt atttgattac tatggagcag aagatgctaa atacgtatta
     1141 atagctatgg gttcagtaac agaaacaata gaagaaacta tagattactt aaatgctaag
     1201 ggagaaaaat atggtttagt taaagttcat ttatatagac cattctcaat gaaacatttc
     1261 ttagatgcta tgccatctac tgttgaaaga atatgtgtac ttgatagaac taaagagcca
     1321 ggttcaactg gtgaaccatt atacttagat gtacgtgatg tattctatgg aaaagaaaat
     1381 gctcctatga taataggtgg aagatacgga ttaggttcaa aagatactac tccttcagat
     1441 ataaaaactg tttttgataa cttagtttca gaacaaccta aaaatggatt tacagtaggt
     1501 atagttgatg atgtaactaa tacttcttta actccatctg agccaataaa aatagcttct
     1561 aaaggaacta taagatgtaa gttctgggga ttaggttctg acggtactgt tggagctaat
     1621 aaacaagcta tcaaaataat cggtgaccat actgaaaaat atgctcaagc ttactttgat
     1681 tatgactcta aaaaatctgg tggtataact atgtctcact taagatttgg tgatactcca
     1741 ataagagcta cttatttaat agatgaagct gattatattg cctgtcataa acaatcatat
     1801 gtttatcaat atgatttatt aaaaggtctt aaaaaaggtg gtacatttgt acttaacact
     1861 atatgggacc aagcaggact tgaagaacat ttaccagctc atatgaaaca atatatagct
     1921 aaaaatgata taaaattcta tacagttaac gctgttaaac taggacaaga aataggtctt
     1981 ggaaacagaa taaacatgat aatgcaatct gcattcttta aattagctga aataatacca
     2041 gaagaagatg ctgttaaata cttaaaagat tctatagtta aagcttatgg taaaaaaggt
     2101 gaaaaaatag ttaacatgaa ctatgctgct gttgatgcag gaataaatgc tttagttaaa
     2161 gttgaagttc cagcttcttg ggaaaatgct gttgatgcag ataaaggaga agttaaagaa
     2221 ccagaattta taaaaaatat actaagacca atgactgcac aagaaggaaa caacttacca
     2281 gtaagtacat tcaatggtat agaagatgga acattccctt gtggtactgc agcttatgaa
     2341 aaacgtggta tagctgttga tgttcctgaa tggataatag ataactgtat tcaatgtaac
     2401 caatgttctt ttatatgtcc tcattcatgt ataagaccag tattagttac tgaagaagaa
     2461 ttagctaatg ctccagaagg atttgaagct aaaaaagcat taggtaaagg attagaagga
     2521 ttaaaatata gaatgcaact tagtccactt gactgtacag gatgtggaaa ctgtgctgac
     2581 atatgtccag ctaaagaaaa agctttagtt atgaagccaa tagatactca acttgataca
     2641 gagttagata attgggcatt tgcagtaaat cctgatgagg tagctccaaa aggtgatgtt
     2701 atgcctgcaa acactgttaa aggaagccaa tttagacaac cattaatgga gttttcagga
     2761 gcatgtgctg gatgtggaga aactgcatac attaaagtag ttacacaatt atatggagat
     2821 agaatgatga tagctaatgc tactggatgt tcttctatat ggggagcttc tgcaccatca
     2881 attccttata cttgtaacca tgaaggtaaa ggtccatctt gggcaaactc tttattcgag
     2941 gataatgctg aatatggatt tggtatgtac acagctgtta agcaaataag aaataaaata
     3001 gtagatgcta tgacagagtt agtttctatg gatatctgtg aagatgctaa agcagtattt
     3061 acagagtggt tagattctag aaatgatgga gaagcttcta aagtagcaag tgctaaggtt
     3121 gttgaattat tagaaaaacc agcttgtgat tgtacagatg aaaaagctaa agaattagta
     3181 aaagctataa aagatagaaa agattatcta gttaagagat ctcaatggat actaggtgga
     3241 gacggttggg catatgatat cggttacggt ggattagacc atgttcttgc atctggtgaa
     3301 gatgtaaatg tacttgtatt tgatacagaa gtatattcaa atacaggagg tcaagcttct
     3361 aaagctactc cagctgctgc tatggctaag tttgctgctt caggtaaaaa atctagaaag
     3421 aaagatttag gaatgatggc tatgacatat ggaaacgtat atgtagctca agttgctatg
     3481 ggtgctgata aaaaccaatt tatcaaagcc gttatagaag ctgaaaaaca tgatgggcca
     3541 tctttaataa tagcttatgc tccatgtata aaccatggat taaaagaagg tatgggaaga
     3601 actgtagcta acgaagcaca agctgttgct tgcggatact ggcatttata cagattcaat
     3661 ccagaagtaa aagctgaagg taaaaaccca ttcactttag attcaaaaga accaactgca
     3721 agcttcaaag atttcatact tgcacaagta agatattctg ctatagctaa acaattccca
     3781 gaggaagctg atggattatt tgctaaagct gaagctgatg ctaaagaaag atatgaagga
     3841 tacaaaaaat tagctgaata atattaaagt attataatgc ttatataaat tgataaaaga
     3901 gagtgtctca aagtgagctt tttagttcat gaggcactct ttttgtatga aatcaaatat
     3961 attttaatca tttcaacaat gaaaaaggac ttatctctat tttgagacag ccttttttta
     4021 tgaagatatt aagattttgt ttttacctat taaatatctt ttttaagttt tccattttca
     4081 tcttgccaat actttttata tattgttcta cacactaaaa ttaagagaac aaataatatt
     4141 tatagtatgt atatttggat ttgataattt taaaatagat acaaaagagt atctcatttg
     4201 tgttttttat cacttagaga tactcttttg atatgtatgg aaaaagagta ttttatccta
     4261 caaagatttg tgtccaataa attgaaccac cacttttttg tgctactcca acacctattt
     4321 tactgaaatt tggattcatt atattttttc tgtgaccttc agaattcatc catgcgttta
     4381 caacttcttt aggagttttt tgacccatag ctatattttc acctgctgtc ttatagctta
     4441 ttccaaattt tttcaacatg tcaaatggag aaccataagt tggagaattg tgtgaaaaat
     4501 agttgttatc aatcatatct tgagattttt ttgtagcaac atttgatata gaagaatcaa
     4561 gagtaagagg atttaatcca gctttagctc tttctatatt tactaagtca acaacttctt
     4621 tttggtaagc tgaaaaactt tcacttgtag agcctgagtt gttattatca tcaggtttgt
     4681 tagtagagtc tgaattattg ttgtcttcag gtttattaga ctcaggttta ttgttatcag
     4741 gtttatcagt agagcctgaa ttattgttgt cttcaggttt attagactca ggtttattat
     4801 tattaggttt attagtagag cctgaattat tgttgtcttc aggtttatta gactcaggtt
     4861 tattgttatc aggcttatta gtagagtctg aattattgtt gtcctcaggt ttattagtgt
     4921 tgttgcaatt cttgtcatta tgattcttat ccttacaaga gttatcttta tcaggcttaa
     4981 gttctggttt tacagaacaa tttggttttg cagaaaactt gtatttagta ttactatata
     5041 ctaaactttt gttacttata tttacattat tttcaagtga tgaagcactt gcagtaacag
     5101 cacttgaagc tgttgctaaa gttaacactc ctaaagctaa tatagttttt aattttgttg
     5161 gtatatttct tctcatcatt aaacacctct taatctaaaa attctaaaaa tgttactgat
     5221 ttgtaacctt tagtgtaggg ggttacccgt gaatctatac taacataaga ttaagacttt
     5281 ttttactgta aagatatgta aaaaagatat atatggaata aataggtata tttcagtgtg
     5341 tttgacaatt tgtattaaaa gcctgtatag tatggaatta cttagttaga gaaaataata
     5401 tttttataat aattccataa cgtaaattta tagttatgat aaattttttt tatttggtta
     5461 aatttattca gtagatgctt tcgtattagc aacaaattat aattatttaa atctttaaga
     5521 aataaaaagg gtctgtctca aaatagagat aaggcctttt ttattgttga aatgataggc
     5581 atatgttatt tcatataaaa agagtgtctc attaagtaaa aagttcactt tgagacacca
     5641 cctttcatat tatgaatttt aatttgtttt atattaatac attagtaatc ctaatgtaat
     5701 tacaataatt tctgctatta gtgaaccaac tgcaactaca atgaaagttt ctttaaatcc
     5761 agttttatgt gttaacttag ataagcttaa gaatgtaaga aaaacaccac tttgtggaac
     5821 tatagttagt atatttgaag ctattgtagc aacacggtga attaattctg gatttagacc
     5881 agtacttaaa taataatctg caaagtttgg cattactatt cctaatgcac cagaagagga
     5941 ccctgttatt gctgacattg ttgaagtcaa aacagtaaga cttataagtg gactacctgg
     6001 tattgacatt ataagtttgc taaatacatc aaaaccaggt gcagccatta ttactgctcc
     6061 aaatgcaact gcagaagatg tagcaaatat aggacctata gaaccactag cacctatgct
     6121 caatgtctgt atcttagatg atagaaattt atggaataaa aaagcagata aaagaattgc
     6181 aactattaaa gctatgtaaa ttacattttt tttcataaat tctgaaccaa aagtacttcc
     6241 agttatagct ataattacga gtgctaatag aggaaaaata cttgcaaaaa atcctggtaa
     6301 ttctctatct aaagattctt cttctaattc tgaagtgtaa gttgcataag tttcaccttt
     6361 tctaatactt ttgtttagag catatttcat atagacaagt ccaaacgtag cacaacctat
     6421 actacctaca atacttggta tagtagctgc agtaagagac gtatttagat attgaatagg
     6481 tataacattt tgaatggctg gtgttccagg aattatagtc atagtaattg ttgcaatacc
     6541 aagccaaagt ggtatctgaa taaggttcca agaaatatca agttttttaa atagagttct
     6601 tgcaagtgga atgactgcaa acataactac aaataagctt ataccaccat acgttaatat
     6661 aaaacttact ataaatatag caactaaaac tctataagga tgattagagc caaatttatt
     6721 taaaataaaa tcagctattg atacagtagc tccactttct tccataaatt tagcaagtat
     6781 tgagcctaat aagaatattg caaaataact cattatatag tttccaagtg cacccatata
     6841 attgtttttg tcagcaccta gcattgatgt tacaacatcc attttattta aaataacaac
     6901 tattaaagtt gctattggag cagctatagt tatatgaatt tctttaactg aaaaatatat
     6961 aatagctatg ataccaataa ttataccaat tggcgcgata atactcataa atatccctcc
     7021 ttagtagaag gacctgaaga atactttctt caagccctaa atataataac tattgtgcag
     7081 tatatcctcc atctagtaag caagcttgtc ctgtaatacc cttagcatta tcacttgcta
     7141 agaacatagc atagtcggca atttcttgaa catctaatag gcgtttttgt ggtacaagtg
     7201 ggtataaaac ttcctctaag acattttcca atggtatatt acgagttttt gaaaggtcat
     7261 tgaactgacc tctgacaagt ggagtatcta catatccagg gcataaagca ttaactgtta
     7321 taccagaatc ggcagcttca agagcagcaa cttttgttag tccaataagt ccatgttttg
     7381 ctgagttata agcagccttt ccagcaaatc ctattacacc attgattgag gccatattta
     7441 ttatacgacc aaagccttgt tttttcatta taggaaatgc ttttttagta gctataaaag
     7501 gtgctgtcag cattattttt atcataaatt caaatttatc agttgggaaa tcttctatca
     7561 tagaaacatg ttgaagtcca gcattgttta ttaatatatc taatctgcca tacttttcaa
     7621 cagttttgtc tatagcgtgg ttaatttctt cttccttagt tacatcacac tttacactca
     7681 tgcaatcata accttttttt tgtaactctg ttatggtatt atttaaagct tcttgattta
     7741 tatcagagaa cattacttta gcaccatttt ttaagaacga ttcagctatt tgtttaccta
     7801 ttccacttgc agctcctgtt acgaatacaa ctttatcttt aaccattttc atttcctcca
     7861 attttatttt atttttatat taatatttta agcaacacaa atttatatgt aatacgtcta
     7921 agagattttt attttctagt tttaattatt ttaaacaaca gatatttttc catttaatga
     7981 atctgacaat attaaaggag cttcagtagc agaaataaca tcatctaatg tgaaagctgg
     8041 atttatttct ttaagtaaaa ttccatctga tgttacttcc ataactccca tttcagtaac
     8101 tatcaaatct acagcatttg ttgcagtaag tggaagagta caattattta atatttttgc
     8161 agaacctttt gaagtatgtt ccattgctac aataactttt ttagctccag ttactaggtc
     8221 catagctcca cccatacctg gaaccatctt acctggaatc atatagtttg ctatattgcc
     8281 atatttgtca acctgaagtg cgccaagaac agttatatca acatgacctc ctctgattat
     8341 tccaaaagaa gtagcactat caaagaaaca tcctccagga agtatagtaa ctggttgacc
     8401 tcctgcattt acaatagttt catcacttga atttccatct tcggctgaac ctaaacctaa
     8461 aaagccattt tcagattgta agataacgtt tacattatcc ggaatataat ttgctacttt
     8521 ggttggtaga cctattccaa ggtttacaac ggctccgtct tttaattctt tagatactct
     8581 atttgcaata tattcttgca tttctaattt atccattata aattacctcc ttcaacgatg
     8641 taattcacaa aaatacctgg tgtattaact tcatttgggt caatatcacc aatttcaacg
     8701 atttcatctg cttcaactat tgtaattttg gcagcagatg ccattaaatt attaaaattg
     8761 ttcatagagc ctttataaac tagattacct gctttatcga cctttgatgc aaatagaaga
     8821 gcaacatctg cataaattgg tttttcaaga aggtattctt tgctatcgac aacaattttt
     8881 tgctttccct cctcaacaag agtaccaata ccagtcggtg tgagtatacc tcctagacca
     8941 taaccagctg cacgtatttg ttcagcaaga gttccctgag gaactaactc aacatctgtt
     9001 tcattgttgt tcatttgtct tccagtttct ttatttaatc ctacatgaga agcaattatc
     9061 tttttaaatt gcttatttac aaccattttg ccaactcctt tgtcaataaa gccagtatca
     9121 ttgcaaatta gtgtcaaatc ttttatattt ttactacata aataatcaat taaattttct
     9181 ggtgaaccat tggccatgaa tccaccaacc attattgtat ctccatcttt tacatgagat
     9241 agagcttcat caatgctaac tattttattc atatttcctc ctatcgtgaa accaccattg
     9301 atattccttg accaccacca atacaaagtg ttgcaagtcc agtttcaact ttacgttttt
     9361 gcatttcata aattaaggta acaagtattc tagctccact tgctcctatt ggatgtccta
     9421 atgctattgc accaccatta acatttaact ttgatgaatc tatttgaagt tcatttttaa
     9481 cagctaatgc ttgggcagca aacgcttcat ttgcttctat taagtctata tcattgatgc
     9541 taagtccagc ttttttcagt gcttttctag ttgcagggat tggaccagta cccatgactt
     9601 caggttcaac accagctgaa gcataggatt ttattttagc aagtggtcta atacctagtt
     9661 cgtctgcttt ttgttggctc attagtatta acatagccgc accatcatta atacctgagg
     9721 catttccagc tgttacagta ccatcttttt tgaatgcagg tttaagttta gataaacctt
     9781 caaatgtcat acctaattta ggatattcat ctttatcaat agtttctatt tttcctcgac
     9841 gtattaaaac atctactgga acgatttctt ctttaaagcg gttgtttttt atagcattct
     9901 cagccttatt ttgactttct aatgccaatt tatcttgcat ttctctagta aattcaaatt
     9961 tagttgcaat attttctgca gttattccca tatgatactg attgaaagca tcaataaggc
    10021 catctgttag catactatct accatggtta tgttacccat tttactacca aagcgagcag
    10081 ttggaacgat ataaggagct tgactcatat tttcagtacc acctgcaatt accacatcat
    10141 tttcaccaga tgtgattgat tgggcagcta gctggacact tttaagccct gaaccacata
    10201 atttatttac tgtgtagctt ggaacagaat ttggtatacc tgcatttata gcaatttgtc
    10261 tagcaacatt ctgtccaagc cctgtttgaa gaacatttcc aattattact tcatctattt
    10321 cagataaatt aagtcctaca cgtgatatcg cttctttaac agcgatagtt ccaagttgaa
    10381 cagctgaagt atttttaaat acacctccaa aacttcctat aggtgttctg actgctgata
    10441 cgattactac atctttcata tctaatagtt acctttcttg aatgataatt aaaaattttc
    10501 tgaataaaga aattagggaa cactataata aagtactttt ttaatacaag cttgttttta
    10561 agtatttcaa ttacttttat tcctaatgct ttaggttttc ctgtttctaa ttatagatta
    10621 cggcgaaaaa agcattttgt ctaagtctaa ttttttatat agtgtataag aaaaatttat
    10681 aggataaaaa tttatagtag atttagaaaa atattttgtt aaaatattaa tgaaattatg
    10741 ttgattttcc tataaaatat cattagaaga aaaaatataa tctaaattat acttttgggg
    10801 aagggggttt aaaatggata taaaacagtt gaaatatttt gttgaaatag tgaaatctgg
    10861 ttttaattta tcaatagcat ctaagacact tcacatatct caaccagcat taagtcaaat
    10921 tataaaaaat tttgaagaaa gtgaagatgt atatttattt gaaaggtata aaggcaggtt
    10981 aaatgggtta acaccagcag gagaaagatt ttatgtaaat gcagaacata tcataaatga
    11041 atacaaaaat atgatggaag atttaagaga agattcagtt caatttaaag gaaaagttag
    11101 aataggaata cctccactta tacttggaat agcattttca gatgtagttg cacagttagt
    11161 tgcaaataat ccagatatag aatttgatat agtagagaag ggtgcatatg acttaaatag
    11221 aatgcttatc ttacaggaat tagattatgc agtattgtta catccacata aaattgataa
    11281 aagtgtagta actgaacatg tgcttcaaga agatgaactt acagcatttt taaatataaa
    11341 tcatccactt gctaagaagg ataagataga ttggaacgat ttgaatgagc agttacttgc
    11401 aatatttaat cctactttta tgatacatca taagctaatg caaaaattta ctgatgaaaa
    11461 tgttaagtta aaaagatata taatgtctgg ttcatgggat tttttgttgc tatcaaccac
    11521 aaattctgat tttataacaa ttctaccatc accagttcat gatttttttg aaaatagcaa
    11581 aattatagaa aagcctttta atcatcctat tacatggaaa gttatattat gtagacctaa
    11641 aaaagaacgt tatagccatg tagaccaaca tgtatttaaa tttattatag atttctttag
    11701 taaaaagaac tcttaaaaat gttattaaaa taacaaataa ataaatcaat ttttattata
    11761 ataaactatt agtttttcat ctatatgttt gagagcaata ataggcttta cataacatgg
    11821 tagagtagta tttaaagtta taatatacaa aatatgtatt tttttaaacg aaaaataaaa
    11881 acagtgtata tctttagata atcactgttt ttattttcta tataaaattt gttaagtatt
    11941 actagaaaat tttttgctta ttatagtcaa actcatgact gttaaaaata taagtaatcc
    12001 tggaaataca gctaaatata acttgtcaaa aatatgtctt tgagcatctt gaagcatatt
    12061 accccatgat gcaagaggca attggattcc aagacctaaa aaactaagag cagattcagt
    12121 taagatagca ttagcaatat ttaacgaacc agcaactaca atagtctctt ttaagtttaa
    12181 aactatatgt cttaatagta tatttctagt cttgatacca gtagctttag cagctagaca
    12241 gtattcattt tcctttattt ttaatgtttc gccacgtaca attcttgaaa cttccatcca
    12301 acttaagaat ccaataatta gtataatagt ggaaacttta ggtgttagat aagtatttaa
    12361 tacaatcaaa actaagaaac ttggtatact cataagcata tccactaatc tcatcaagaa
    12421 ttggtccagc ttacctccaa tatatccact aataacacct acagcagtac caaatactac
    12481 agaaataagc attgataaaa taccaacact aagtgaaatc cttccaccat aaagagctct
    12541 agagaagtaa tctctaccaa gttcgtctgt accaaagata tgctttaagc taggttcttg
    12601 aagtttagaa gtagtatcaa ttgcattaga gtctatattt aatataaaag aagctataat
    12661 taatatgatt attatgctaa gagtaataca tgcaaaagca aaggatttat ctttttttaa
    12721 tttttttaag atacttgtaa acacaactag aatcctcctt tgctagattt tattttaggg
    12781 tcaattaata agtatagtat atcagcaact aaattaccta aaattaaaaa tagtgctgat
    12841 aacatagtta ttgccataat tacagtataa tctcttgaaa aaattgaatc aatcattaat
    12901 cttcccatac ctggccaaga gaaaactact tctgtaacaa atgcaccagt gactaatttt
    12961 tgtatactca tgcctaggat tgtaaccaat gatgtcagtg aatttttcaa gacatgttta
    13021 aatagtatga ctttttctga taatcctttt gctcttgctg taataacata ttgttttttt
    13081 aattgttcat ttacactagc ttctacataa tttgtaaaaa tagcagtatt atatagacct
    13141 aaagttatta caggcaaaac taaatgtttt actaaatctc caatactatc atttccaata
    13201 gtatgcatac ctcctgatgg gaaaatgtta agtttaactg aaaaaattga aatgagtatc
    13261 atacctatcc aaaaagaagg tatagaaaca cctatataat taaaaacaga tgatacttta
    13321 ctagttattg aatttttatt tacagctaaa aatattccaa gaggtatagc taccaatatt
    13381 gaaaatatca aagaagtacc cataagaata aatgtatttc ccattttttc cataatttgt
    13441 tctgtaacgg gcatatggtt tatataagaa atacctaagt ttccacttaa agtgtttttc
    13501 aaccagttta aatatcttat atatactggt ttatcaagtc ccatattaac ccttatattt
    13561 tcaatatcct gtgaagacat taaaggattt atatatgcat ctgctgggtc accaggagct
    13621 aattctaaaa gtccaaatga taatatagat attaagatta acataggtat taaaactttt
    13681 actctattca atattaattt taacataagt aaattctcct aattaaaaga gatgtctcgt
    13741 tttaaaataa taaaacaata ttatttttag acatctcctt ttctaattaa taatataaaa
    13801 ttttacatca attattctac atacagttta gaccaatctt caaataatat tgcaggagat
    13861 ggcttagcat cttttattcc ttttagattt ttttgagctg ccattaaatt ttgttcataa
    13921 tctatagtgt aaataggcgc atccttagat atatcctgtt gtatttgctt atatatttct
    13981 tcacgttttt ccttgtttaa ctcagtaaat cctttttccc ataaagcatc tatttcagca
    14041 ttagaatatt tagtttggtt gcaatattga ccagtttcaa acatacctct atatgggttt
    14101 ggttctaatc cccaagcata accatttaca tacatatcac attcagtgct attactaaat
    14161 agtatattga agaatgcctt actttcataa ggaactattt cagcttctat accaattttt
    14221 ttcaactctt gttgagctac taaggcatag tttttatgcc caaatctacc tgtgttataa
    14281 cctattttta atttatcaat tttaacacca cttttatcca ataagtcttt agccttattt
    14341 gtatcttggt catatccttc aacaccttct tctgtataga agtctgcttc tggaactaat
    14401 atagatttag caggtacaga accttctttt ccatatgctg attctatcat ttcatttcta
    14461 tttaaggcat atgataaggc ttgtcttact tctttgttag ccatgtttga tatattttgg
    14521 ttaaatacaa tgtagtttaa tctttcttca ctataagtat aagtttgtaa gtttgagttt
    14581 gattttgcct tttcatatcc ttcagcagat gtcttcataa gtgaaatttc gccattattt
    14641 aaagctgctt cttgagatgc ttcattagga attattttta gcgctataga atctgcctta
    14701 ggttctccac caaaataatc tgcattcttt tcaaatacta tgctttcacc ttttttccat
    14761 tccttaaact tgaatgcacc agaaccaact ggattattat ttttttctga cttagctata
    14821 tttgattctc cttcaaacac atgttttggt attggagaaa ttttagatat accatataag
    14881 aaagattcac ttgcagttgg taaagtaatc ttaactgtta aatcatcaat tttttctact
    14941 ttaactggtt tctcacccac caataagtat tgtcttgatg gtgaattttg tttttcgtct
    15001 aaaactgtat taaatgtaaa taagacatct tcagcagtta ttggctttcc atcatgccat
    15061 tttaaattgg attttaattt tatagttaat tccttactat ctttaaaatc tactttttct
    15121 gctaaataat ttactaaatt tcctttttca taagtataaa gaggagcata aactatattt
    15181 gatgcaagag agtcttcttt aacgtttgca aaagctccat tgattatatc tgggtcactt
    15241 gccataggta ttacaacagt tccaccagat ttgcttccat tagacttgtc tttatcattg
    15301 gaagcacagg ctgtaaatgt aagcattaat gatgaaacta agattaatga tgccaatttt
    15361 ttaaatttca tattcttata aaacctccat aaaataatat ttaacacata ttttttattg
    15421 tataatagta atcattatta aatcaatata tatagaaatt aactattata ttattataat
    15481 taatagaaaa aaataataaa atgtaagaaa atgttgtaat ttaaattttt ataaaataca
    15541 atcaaaaggt gtatgttatc ttaagtaatt ttgctgagag gagaaaataa tgagtaaagc
    15601 actaattgag gttaaagatt taaaagttta ttttcataca gataaaggga ttgtaaagag
    15661 tgtaaatgaa gtaagtttta atataaatga aggtgaaact attggaatag ttggagaatc
    15721 aggatgtggt aagtctgtaa cagccatgtc tttgatgaag ttacttccaa catcaaaaat
    15781 agaaggtgga gaaataatct ttcgaggcaa agacatttta aagatgaatg aagatgaact
    15841 tatgggaata agagggaatg agatatcaat gatttttcaa gaaccaatga catctttaaa
    15901 cccagcgttt actgttggaa gtcaaatcat tgaaggtatt atgatacatc aagatttatc
    15961 aaaggaagaa gctaagaaaa aggtaatcga tatgataaaa ctggtagaaa taccaagagc
    16021 tgaagaaata tataattcat atccacatga attatcaggt ggtatgagac aacgaataat
    16081 gatagctatg gctttatcat gtaatccaaa attacttata gctgatgaac caacaacagc
    16141 tttagatgtt actattcaag ctcagatttt agatataatg aaaaatataa aagaaaaatt
    16201 aaacacatct ataatgatga ttacacatga tttgggagta gttgcagaaa tgtgtgacaa
    16261 agtattagtg atgtatgctg gtaaaattat agaagtggca gaggttgttg aattatttaa
    16321 aaatccaaaa catccttata ctattggact tctaaaatca aagcctgctc ttggaaaaaa
    16381 caaagataaa agattgtatt cgattccagg acaagtacca aatccaattg gaatgccaga
    16441 ttcatgttat tttagtgata gatgtgaaaa agtatgtgat aaatgtagaa ctcagatacc
    16501 tcctttaata gaattgaata gtggtcatag tattgcatgt tggttatata aaaaggagga
    16561 gatataattg aaaaaagaag agagtttatt aaaagtagaa ggtcttaaaa agtactttga
    16621 agttaaatct ggattatttg ataaggaaat taaatgtgta aaggcagtag atggaatttc
    16681 ttttgattta aaaaaaggac aaactttggc tatagttgga gaatcgggat gtggaaaatc
    16741 aactgcagga agaaccattc ttagattaat tgaaaaaact gaaggtaaag ttgaatttaa
    16801 tggtgtagat atatataagt tagagaagga agaacttaga aagttaagac ccaaaatgca
    16861 aatgatattt caagacccat atagttcatt aaatccaaga ttaaatgtgg aacaaataat
    16921 atccgaggca gttatagaac ataatttagt gccaaagaat gaaataaaag ataatatatt
    16981 aaatgttgta gaaagttgtg gtctttctaa gtaccattta aatagaaaac caggtgagtt
    17041 ttctggaggt caaagacaga gaataggtat agcaagagca ttagcattaa atccagaatt
    17101 tatagtttgt gatgaacctg tttctgcact agatgtttct attcaatctc aaattataaa
    17161 tttacttatg gatttacagg ataaatataa actttcatat ttatttatat ctcatgattt
    17221 gagtgtagta aatcatattg cacatagagt ttgtgttatg tatctaggtt ctataataga
    17281 aattgcaact aaaggagaac tttacttaca ttctcaacat ccatatacaa aagcacttat
    17341 gagcgcagta ccaatacaag accctacaat taagaaaaat agaattatat taaaaggaga
    17401 cataccatct ccaacaaatc caccaagtgg atgtaagttt catacaagat gcccttattg
    17461 taaggaaata tgcaaaaaag agattccagt tttaaaagaa atatcaagaa atcattttgt
    17521 tgcatgtcat ctagtataga tatttcaaaa attaaatttg atgtatataa taagaataat
    17581 agaataaact gaaaattttt ttgattaaga tgggatttca tgctgttaat attaagttag
    17641 ttgatatata agtttaatct ataatattat tattgaaaag agaggtagtt atgtcaattt
    17701 tagtaacatt gttgggatta ttggtttgta caacgatttc aactgtattt agcaaaaagt
    17761 ggagtaatat tcctcttgca atatatcaaa ttgtactagg gattatattg tctattttgc
    17821 catttaaatt ctcattttca tttaatccag aaatatttgt aatatgtatc atagctccat
    17881 tactattcag tgaaggacaa aatgtatcga gaaaagagct attagagctt agaaaaccaa
    17941 ttttgcttct tgcatttggt ttagttttaa taaccgtgtt tgcaggtgga atctttatac
    18001 attttcttat tcctgggatg ccattatctg tttcatttgc tttggcagca gtaatatctc
    18061 caactgattt agtagcagta aaatcaatta cacaaggatt aaattttcct aaaaatatga
    18121 tgagtatact tgaaggtgaa tctttattaa atgatgctgc tggagtagta gcatttaagg
    18181 ttgcagtact tgccacagtt acaggagttt tttcaataga agaggcaggg attcaattta
    18241 tgattacagc ttttggagga ataatagtag gaagtatttt aggatacata attataaaaa
    18301 taagacttag tttacacaag tggaatttgg aagaaattcc aatggtaata gttatacaaa
    18361 taatgactcc actttttgta tattttgtag cagaagaaat aggagtatct ggaatcctag
    18421 ctgttgtaat ggcagggata gctcatggaa ttgaaaaaga gcatttacaa aatacaacta
    18481 ctaaattaag gataatatca gacaatactt ggtatgtttt ggagtatgta ttgaatggat
    18541 ttgtctttac actgcttggt tttttgttac caagtatata tacaggactt agtagtaaaa
    18601 atgaaaatat ggcattggaa ttgactttta tttctacttt aattgtactt attttatttg
    18661 ttataagatt tatatgggta tatttatggc ataattcttt tataaaacct aaaaagaatc
    18721 ctttaaataa tttttttgta ggttttttag gttttaagga tgaagaagtt gtaaaagaga
    18781 gtatttctaa atgtaaatac tcactaatag ttgctacttg tggtgttcat ggtacattta
    18841 cactagcaac tgccttatct ataccttttt atttggctga taaagatgta tttccaatga
    18901 gagatactgt tttatttata tcatcagaag ttattcttat aagtttagta ttggcaacag
    18961 ttttactgcc aagattatta aaaaataacg tacaagagtc agaaccttta ttatctgata
    19021 aagaggcata taaactaatt ttgaaagaag ccattttaaa acttagtaat gaaaaagcac
    19081 aagaagtaca accagttata catgatttaa atgaacagtt ggtagactca gaaaaaggaa
    19141 tactaaatac tccagataat aaacttataa gtatgatgat tgcagaagca gaccaaaagg
    19201 gattattagc tgttttgaaa ctattagaag atggtaaaat ttctgataga gcctttaaat
    19261 tgtatagatt ttatattaca aaatcaagaa aatctgttca aaaatctgta tttaaaattg
    19321 taaaattaaa aataagcatg tggattataa atagaaaagt aaagaaagaa gtaaaagaaa
    19381 aaattacaaa aatagtatct gagaataaag aaataatcaa agagtttcac catgcatatg
    19441 cactttctac taaaactgct atatcattta taaataaaaa tactgatgaa gataacagac
    19501 atgaggcctt aatagcaatc agattctata atcgttattt gaattggatt actagaaaga
    19561 ttcaaaatga tgataagttt gaggcaagag tagaatacta cagagtaatg gcgatacaaa
    19621 gtcaaagaga cagtgttcaa caattagttg aaactaagca aatatcaaga gaagtggcta
    19681 aagagttatt ggaaaatatt ctctatgatg aaatgatgat gatagaatca taaatataat
    19741 ggaataaaat ttgatttttg atgttaaaat taatactaaa aaataggaaa tagttttcaa
    19801 taatttactt gacatatata tttatttata atagaataaa accataaatt gaatacaata
    19861 tataagcgtt aacaagctgg attgttactg gtaagcaggc aatacctagg ttaaggtata
    19921 caggtgaatt gtatcttaat ttaggtattt ttttatgaat aagggaaatt gtattttggg
    19981 agagatattg attatgatta ttcaacaaat ttataataat aatgttgtat tagttttaga
    20041 tgaaaatata aaaaaagaat taatattaac aggatgtgga attgggtttc aaaaaaagaa
    20101 aggtcaagaa atagataaaa gtaaaattga aagaactttt gtaatacaag atgaaagctt
    20161 tttagataaa attagtaaac tagcaagcca ggttgatgag aaattttttg aggttagtac
    20221 tgaaataatt gcttatgcag aggagaattt gaatactaaa ctttatgaat atatttatgt
    20281 agcacttact gaccatattg catttgcaat taaaagatat catgaaaaca taactataaa
    20341 gaatgattta ctacatgaga taaaaagaat acataaaaaa gaatatgaga ttgggaaatg
    20401 ggctgtagat tatataaata aagaatttga tgtgaaattt ccagtagatg aagctggttt
    20461 tatagcaatg catatagtaa attcaaacta taagggaagt tcaaaggaat cactgttaat
    20521 tactaaaatt gtaaaagaca tactaaatat tataagatat tattatagag ttgaatttaa
    20581 agaagatgat ataaattatg ataggcttct gactcattta aaattctttg caaaacgatt
    20641 agtaaaaaaa gaaaaaatca atgatacaaa taatgaaatt atagatataa ttaaagtaaa
    20701 gtatgaaaag gattatgatt gtgcatataa aataaaaact catgtagaaa agaattatga
    20761 ttactatgta agtcaagatg aactacttta tttaacatta catatcaaaa gagttatatc
    20821 tgtactgaat ttataataaa gag
//
LOCUS       DAEORZ010000063        20735 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEORZ010000063 DAEORZ010000000
VERSION     DAEORZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.063"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            68..3016
                     /locus_tag="KRH39_002079"
     CDS             68..3016
                     /locus_tag="KRH39_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8511979.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            2994..3458
                     /locus_tag="KRH39_002080"
     CDS             2994..3458
                     /locus_tag="KRH39_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8511980.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3571..3873
                     /locus_tag="KRH39_002081"
     CDS             3571..3873
                     /locus_tag="KRH39_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8511981.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            3935..5299
                     /locus_tag="KRH39_002082"
     CDS             3935..5299
                     /locus_tag="KRH39_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG8511982.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            5334..5807
                     /locus_tag="KRH39_002083"
     CDS             5334..5807
                     /locus_tag="KRH39_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511983.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YVKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            5979..6629
                     /locus_tag="KRH39_002084"
     CDS             5979..6629
                     /locus_tag="KRH39_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG8511984.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKYREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6803..7735
                     /locus_tag="KRH39_002085"
     CDS             6803..7735
                     /locus_tag="KRH39_002085"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG8511985.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKNRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            8009..8722
                     /gene="ispD"
                     /locus_tag="KRH39_002086"
     CDS             8009..8722
                     /gene="ispD"
                     /locus_tag="KRH39_002086"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBG8511986.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8719..9204
                     /locus_tag="KRH39_002087"
     CDS             8719..9204
                     /locus_tag="KRH39_002087"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBG8511987.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9299..9507
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9571..11286
                     /locus_tag="KRH39_002088"
     CDS             9571..11286
                     /locus_tag="KRH39_002088"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG8511988.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11408..12853
                     /locus_tag="KRH39_002089"
     CDS             11408..12853
                     /locus_tag="KRH39_002089"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG8511989.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13293..14774
                     /locus_tag="KRH39_002090"
     CDS             13293..14774
                     /locus_tag="KRH39_002090"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBG8511990.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            14995..15150
                     /locus_tag="KRH39_002091"
     CDS             14995..15150
                     /locus_tag="KRH39_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8511991.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15295..16692
                     /locus_tag="KRH39_002092"
     CDS             15295..16692
                     /locus_tag="KRH39_002092"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBG8511992.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            16677..17090
                     /locus_tag="KRH39_002093"
     CDS             16677..17090
                     /locus_tag="KRH39_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBG8511993.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17196..17954
                     /locus_tag="KRH39_002094"
     CDS             17196..17954
                     /locus_tag="KRH39_002094"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBG8511994.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLKEVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            17967..18704
                     /gene="rlmB"
                     /locus_tag="KRH39_002095"
     CDS             17967..18704
                     /gene="rlmB"
                     /locus_tag="KRH39_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBG8511995.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVTKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            18707..19234
                     /locus_tag="KRH39_002096"
     CDS             18707..19234
                     /locus_tag="KRH39_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBG8511996.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            19299..19946
                     /gene="sigH"
                     /locus_tag="KRH39_002097"
     CDS             19299..19946
                     /gene="sigH"
                     /locus_tag="KRH39_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBG8511997.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            20020..>20735
                     /locus_tag="KRH39_002098"
     CDS             20020..>20735
                     /locus_tag="KRH39_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG8511998.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVE
                     DVFSITGRGTVATGRVERGVL"
BASE COUNT         8583 a         2168 c         3891 g         6093 t
ORIGIN      
        1 aagtacaaaa aaaacaatat atggcatata agttgctata taaataataa ggagggaaca
       61 attataaatg aggcaaagaa taaaaaactt aattgaaaat gaagataaga aaaatccttt
      121 aacagatgaa gcgatttcaa gtaggttaaa tattcgaaga gaagatgtta cctttttgag
      181 aaatgagtta aaaatagaag attcaagaca aagaagaaaa gttgttttaa ttaaagctat
      241 aaaagatatt ttaaaagaag aaaaaactat aaataaaaat gatataacaa gaagattaaa
      301 cagcattggg tttacagtat caagatttac agtcatacaa tacttaaaag agattcaaga
      361 ttctggtgac ctaaaacttg ataaagagag tgatgataaa aaagaagaaa taatcaataa
      421 tagagaaaat aaagatatag catttgataa actcataggt agtagtggta gcttaagtac
      481 aattataaaa ctagccaaag cagcaattct ttatccacct catggtcttc atactattat
      541 tatggggcca actggtgttg gaaagagtga gttagcagag tgtatgtata aatttgcagt
      601 agaatcatcc agattcccta agaatgcacc atttatagtt tttaatgcag cagattattc
      661 agagaatcca cagcttttgc tatcacagtt atttggacac gttaaaggtg cttttacagg
      721 agctgatgag aacaaagaag gattagtatc aaaggcagat ggaggaatat tgtttataga
      781 tgaaatacat agattacctc atgaaggtca agaaatacta tttcaactga ttgataaagg
      841 tatggtcaga aagttaggag aaacaagact aacacataaa atagatgtaa tgattatttc
      901 agcaacttct gagccaatag attctcattt acttaataca tttaaaagac gtatacctgt
      961 aacaattgaa ataccagaat tagttgcaag accattaaat gagaggtttg acattataaa
     1021 taatttcttc ttaatagaag ctcaaagaat gggagcaaac atacatataa agtcagatgt
     1081 attaaaagct cttatgcttt acgattgtat aggtaatgta ggtcaactta gaagtgatat
     1141 tcaagtagct tgtgctaggg gactattaaa ccaacttaca aacaaactaa aagaagtaaa
     1201 tataagcata tcagatgtac ctggatatgt aaaacaaggt cttataaaaa tcagaaattg
     1261 tagaggaaaa atagaaaatt atgtagatgg ggatttaatt atagactcaa atcttcaagg
     1321 tgagatagaa aagaaaaatg aagatatata tacttttcca gatgaaatat acaaacttac
     1381 tgaaagaaga catattgatt tattggaaca aggtttagat tttgatgtta taaatagaat
     1441 cattggagga gaattagaaa gttgcattca aaaatacata aaacaagtta aaaaagtttc
     1501 tattagtaac aatcttccag atataagtcc aattgttgga aaatatatcg tagagctaac
     1561 ttatgaagta atcaaaatag catcacaata cctaagttca ctagaaccga atctacattt
     1621 atgtttagca gttcacctaa aagcagcttt tgagagatta aaagaaggaa aagtaatatc
     1681 aaatgctcat ttgaaagaaa taataggaaa ttatccagta gaatattctg tagcagaaaa
     1741 ggtagctcaa tatttaaagg aaagatataa cataaactta ccaaaagaag aaataggtat
     1801 aatagcaata tattttaaaa tgagtagtag aagagatatg tctgaacaaa aaaagatagg
     1861 tgtacttctt atggctcacg gaagagtagc atcttctgta tgtgaggtta caaataaact
     1921 attaggaatt aatcacgcaa ggtatttaga tatgccattt gagaagaaac cggaagaaat
     1981 gattgaagaa gctactacaa taataaaaga tattgatgaa ggtagaggtg tgattatatt
     2041 agtagatatg ggttcacttt catattttgg agatattatt tctggaaaga ctggaattaa
     2101 tataaggaca atatctagat ttgatacatt gctttctata gaggttatac gaagagcagt
     2161 tttaccagat ttaacaatag acgagatagt tgatgaagtt aaaggtatag agccacaagt
     2221 aaaagaaaag gttagtaaat gtaataatac aaaagtagat tttagaaaac ctataatttt
     2281 aacattatgt attactggat tagggggagc ttgtaagata aaaaaaatcc tagaggaaaa
     2341 tattaaaagt ataactgaaa aggttgatat agaacctata ggtatgattg aatatccaag
     2401 tcttgattta gagataaaac aaataagcaa tgataaaaat gtaatcgcca tagttggaac
     2461 aattgaccct cagatagcaa atatcccttt ttttagtcta gtagacataa atgataaagc
     2521 aaagttaaat caaataaata acctaattga tacaaatcaa attggtgaaa caaataaaaa
     2581 atatgaatta tcagatttaa tacataaaga agatatattt gtggacttac aggttaaatc
     2641 aaaagaagaa gttctaaaat atatgagtaa aaaacttaga gagaaaggtt atgtgacaaa
     2701 aaatttctat aaaaaagtag tggaaagaga taacttattt ccaactgaat tatcagatga
     2761 aatagcaata cctcacacag atggaataga tgtaattagg ccagctataa gtatagccat
     2821 cttaaagaag cctattgtgt ggagtaaaag taaagtaaaa ataatacttt tattagctgt
     2881 agaccaaaag tgttttaatc cattaagcac tctactctca tttatagaaa cagatgaatt
     2941 taagaagata aaaaacatta aagatagaga ttttattagg gagatgatat tagatggagt
     3001 caatgaaaat aattagtagc aatttaattt ttaaaaatat tgaagtgtct aacaatgaag
     3061 atgcattaaa gtttttggga caacgtttgt ttgacgaaca gtatgtaaaa gaaagctaca
     3121 tacaagctgt tgtagcaaga gaaaaaaagt atgctacagg gttaccaaca gaaatatacg
     3181 gagtggcaat accacataca gatatagtac atgtaaatga gccaggaata gcaattggaa
     3241 tactgaataa acctgtaaaa tttataatga tgggtacaga cgatactgaa attgatgtaa
     3301 aagttgtatt tatgttagca gtgaaagagc cacaagaaca attgcaacta ttagaaaaac
     3361 ttatgactat atttcaagat aagaatatac taaataatat tgttgactta agtgaagaaa
     3421 gtgtgagtga tttattaaac tctaaattga aaaattaaac atgtaaagta tattaaaaat
     3481 gtaaaattaa acatgcaaag tatgttaaaa aatataaaaa gattattaaa gatttctaac
     3541 tatagatatc tttaaaataa aataatttca atgggggtga ctaatgtggc taaaaagata
     3601 ttagttgcat gtggaacagg agtatgtact tcaacaattg ctataaacaa attaaagaaa
     3661 gcacttcaag acattggtaa attggatatg gttaatatta cacaatgtaa agtagtagaa
     3721 gttgcatcaa aagcacctga ttatgattta ataatttgta ctacacaagt atctagtagt
     3781 ataaaaacac cagtgataaa tggattacct tttttaactg gtgtcggaat ggataaatta
     3841 atcaatgatg tattagaaga gttagaatta taattaaaaa aattaaggaa catggtctct
     3901 aaaatcataa gttttagaga cttaaaatta aataatggag ggtctttata tggaaatatt
     3961 aaatttcatt gttggtctag gtgcacaagt aatgatgcca ataataatat gtatttttgg
     4021 tttgatatta ggtacaaaac taggaaaatc tttaagagct ggtttaactg tgggagttgg
     4081 gtttataggt cttaacacta ttatagcttt gctaacagat aatttaggtc cagctacaca
     4141 acaaatggtt aagaatttag ggctaagttt atcaataata gatgtaggat ggccagcagc
     4201 atcagctata gcttttgcat caacagtagg ggctttaatt attccaatag gtcttgtggt
     4261 aaatatagtt atgctaatta ctaatactac tcaaactgtt gatgtagaca tatgggatta
     4321 ctggcatttt gcttttacag gagctttagt ggcaggggct actcaaagtg taatgtgggg
     4381 agtatttgca gcagtagcaa atatggtaat tgtattggta atggcagatt taactgctcc
     4441 aggtatagag gaatatttag gtatgccagg tatatctctt cctcatggat ttacacaggc
     4501 atttgttcca attgctatag tagtaaataa attattagac ctaataccag gcataaataa
     4561 gattgagata aatgctgata cattgcagaa aaaatttgga ttatttggag aacctttaat
     4621 aatggggtct gtaataggtg taataattgg tattgctgcg aaatatgata taaaaggaat
     4681 attacaatta ggtgtaacta tgggagctgt attaatatta attccaaaga tggcagcatt
     4741 attaatggaa ggattattac cagtatcaga agctgcacag gaatttattg agaagagatt
     4801 taaaaacaga ggcaagattt atataggctt agattcagca gtaggaatag gtcatcctgt
     4861 tacattatca gttgcactag tgttggttcc tttgactata cttatagcag ctatattgcc
     4921 aggaaataaa gttttaccat ttgcagattt agcagtaatt ccatttgcac ttgtattaat
     4981 cgtaccaatt acaaagggaa atgtgtttag aacattaata ataggaataa ttataattac
     5041 ttctggatta ttaatcgcaa ctaacttagc tccattattt acccaaatgg ctttaaatgc
     5101 tagttttaaa atgcctgaag gagcaactat gatttcaagt atttgtgatg gtgcgaatcc
     5161 attaagttgg gtatttgtaa aagttatgaa ctataaagtt attggaggag tagtttttgg
     5221 agttatagct ttaggaatgg ctatttacaa taggaataga ataataaatg aaaacaaaaa
     5281 gctatctata gaagaataag gttaaaacaa ttttattaag gagtgtatag gttatgaaaa
     5341 aatataaata tgattctaaa agatacttac tcagtgttac aataccagga ttttttatga
     5401 ttttaatatt gatttatgca ctgtttaata actatacaaa ttttaatttg aatatatata
     5461 gtttgttaat aattgtctgt acttatggta tattcaatac ttttatatca tcaagtaatc
     5521 ctaaaaaaat tattattgat agcaaatgta tacattttac ctcatttatg actacacata
     5581 agtatgaaat taaagatata gaaaagataa gaataagaga attttataat aaaaaaattt
     5641 atgtaaaaat taatgatgga aaccttttta aaggtaggta ttggataaga acaaatatgt
     5701 ttaataatag tatagcatta tatagctatt tcattgagtt ggaagagtgc ttatatccag
     5761 attcactaaa atttagaaat aaaaaatttg agaataagaa catataaaaa taaaaaattg
     5821 aattgaagcc tagcttaatc ttttttgata agatataagt ttgaacttta tactaaattt
     5881 ttagaatttt caatcaagac aataacataa tagctaagca attagaacac aattaagatg
     5941 tataaggcat gtatatgatg aatgggagga tttaattaat gttattagag aaattaagaa
     6001 aagaagtatt acaagcatct ttagaccttt taaactataa tttagtgaca ttaacaggag
     6061 gaaatgtaag tgggagagat gaacaaactg gctatatagc aatcacccca agtggaatgg
     6121 attatagaaa tcttacacca agtgatattg ttatagtaga tgtagatgga aatattatag
     6181 atggaaaatg gaaagcatca gtggatttat cagaccactt gtatatatat aagtatagag
     6241 aagacataaa tagtataatt catacacatt ctacatactc aagttgtttt gctattttaa
     6301 atgaacctat agaatgtgca agtacaactc ttgcaaatga agtagggggt tcagtaccag
     6361 ttgcaaaatt ttcaccacca acatctaaaa agatgggaaa atgtgttatt gaagcaattg
     6421 gtgacaaaag agcatgtctt ttagcaaatc atggcgtaat tgcagttggt ccatctgtag
     6481 gacatgctct tacagccgct gtaatgttag aagattcagc aaaagtatat tacttagcaa
     6541 aatcaattgg cacaccagtg ctactgcctg atgaagaaat tcaaagagcg agagatgtat
     6601 tttttaatgt ctatggtcag gataaataaa ttagataaaa ttttaaaagt aatatgaaaa
     6661 tatagatatt caatgatatt aaggagattt ataatgatat aataagttta atagttaaga
     6721 agtaaaatat gaaatgtaat agtatataaa ttcatttaag actaaaaaga ataagataaa
     6781 ataaaaagga gtctgtattt ctatgtatta cttattaaac aaaagcacaa atccttactt
     6841 taatttggca ttagaagaat atctattttt aaatgataaa tataatgatg atattataat
     6901 catatggaga aatgaagaat ccatttttat aggtaaaaat caaaatccat atcaggaagt
     6961 atatcatgat gttattgaaa agggagaaat tcctatttta agaagaatat ctggaggagg
     7021 aactgtttat catgacttag gtaatatcaa tatgtcattc atacaaaaaa ataggcaact
     7081 tcatgagata gattttttgg agcacactaa gtttatgcaa aagatgcttt caaccttagg
     7141 actagatgta tctataactg aaagaaaaga tttattttta aatgggaaaa aaatttcagg
     7201 ttctgcacag tctataaaaa gaaaaaattc tctttatcat ggaactcttc tttacgattc
     7261 agatttaaat aaattaacta aatatttaaa ttcaaataaa gcaactgaat caaatgcaac
     7321 taaatctgtt tcaagtaaag ttacaaatat taagttttta ctcgaaaaag atataaatta
     7381 ctttttagat tattgtgttg aatatttaaa atctaatata aagaatataa aagaacttga
     7441 attggatgaa gaagatatta aaagtgtata caatatagga gaagataaat ataaaaagat
     7501 agagtggaca tatggtaaaa cgccgaaatt tcaagtagtg ctaccattta ataatgaaag
     7561 taaaataaat atacatgtta acagatggaa agtatcaaaa ttttttattt cttatgatga
     7621 taatgtaatt aatatggaca agttgttaga tttgaatttc tttaaagaag aaataaaaga
     7681 gagtatttta gaaaattatc cacattacat agatttaatc aatttaatat tttaatataa
     7741 aacctctaat ttgataaaat tgtcttatat caaactagag gtttttaata ttcttgcata
     7801 ttaacaacca ttacaaaatt gtaaaattaa aaacaacact attgaaaaat caatatatat
     7861 tgtaataaat atatgtgttt ttcattagca aatagacatt aactatttat acaactgaaa
     7921 atttaatata taattaatca gatggttaaa ataaaaatat taaaagtttt aataaatgat
     7981 ggttaaaaat aaaatacgag gtgataccat gtatagtgtt ataattgttg cagcgggaag
     8041 cggaaggaga atgaacttag atataaacaa acagttcata aaattaagag aaaaagaaat
     8101 tattgctcat acaattcaag ttttttatga aaatataaat atagatgaaa tagtggtatg
     8161 tataaaaaaa gaagaagaag atttttttaa agaaaatata ataaataagt ataactttaa
     8221 aaatataaaa atagcatatg gcggaaaaga aagacaggac tcaatatata atggtttaaa
     8281 aaaattagat gaaaattgtg atatagtact tatccatgat ggagcaagac catttgtcga
     8341 ccatagaata atcaatgaat ctataaaggt agctaaagaa aaaaaggctg tggttgtagg
     8401 tgttccagta agtgatacta taaagatagt gtcagatgga acagtacaag aaactccaga
     8461 aagaaatttg ctgtgggcag cacaaacccc tcaaaccttt gaatataatc tcataataga
     8521 tgcttatgaa caagcatata agaacaatta ttatggtact gatgattcaa tgttggtaga
     8581 aaatataggt caaagtgtca ctatggtaat gggttcttat gaaaacataa aaataactag
     8641 tccagaagat ttaaatatag cagaacaaat attaaatatg gaaaaaagag atgaagttaa
     8701 cagtagaagg agaattatat gagaatagga ttaggatatg atgtacacaa attaacagag
     8761 gatagaaaac ttataatcgg tggtgtagag ataccacacg ataaaggttt gttgggtcat
     8821 tctgatgctg atgtattgat acatgcaata atggattcta ttttaggtgc tctagcatta
     8881 ggagatatag ggaagcattt cccagataca gatgaagaat ataagggagc agatagtatg
     8941 aaattattag aacatgtgta taatctaata acaagtaaag gatataaaat tggtaatata
     9001 gacagtacta taattgctca aagccctaag atggctccat atattgagag tatgagaagt
     9061 aatatatcaa aagttttaaa cacagacatt gataatataa acataaaggc aacaacagaa
     9121 gaaggtcttg gttttacagg agcaaaacag ggaatagctt cacaaagtat ttgtttatta
     9181 ttgttgacaa gtcagaataa ttaatatatt attagtaata agtttaatgg atatcaagta
     9241 ataggcctat atagaatatc tataatgtat gaatttaagt gaaattaata taaatataaa
     9301 attagctatg ataaagaaat agtaaaatga tgaaatcttc acagagagaa aacggtggtg
     9361 agagttttca agaggaagtc tttgaacctg tcttttagct taaaggtata aaaatcttta
     9421 cggtaggaac cgttatattc ccaaaagaga gctattcata tagctaacta gagtggtacc
     9481 gcggaaatta aacctttcgt ctctatactt gtatagagat gagaggtttt ttatattttc
     9541 aggaagttaa tattcaggag gaatctatta atgaaaatgt caaaaatgtt tatgccgaca
     9601 ttaaaagaaa tcccagcaga tgctgagatt acaagccatc aattaatggt taggtcaggc
     9661 atgataaaaa aaatgacttc aggtgtttat aaccaattac caatggggtt aagagtgttc
     9721 aaaaaaattg aacaaataat tagagaagaa ttaaataaaa aagattgtca agaaatactt
     9781 tgtgcagcct tacttccttc tgaattatgg aaagagtctg gaaggtggac tgctatgggt
     9841 gaagaaatgt ttagactaaa agacagaaca gaaagagagt actgtcttgg gccaacacat
     9901 gaagaagcat tcactgatat aataagacaa gaaattactt catataaaca attgccacta
     9961 aacttgtacc aaatacaagt aaaatataga gacgagagaa gaccaagatt tggtgttatg
    10021 agaactaaga cttttacaat gaaggatgca tatagttttg atgtagatga taaaggttta
    10081 gataagtcat atcaagatat gtttgatgca tatgtaagta tatttgatag atgtggattg
    10141 gagaatagcc ctgtacaggc agattcagga gctataggag gttctacatc agccgaattt
    10201 atggttaagt cagaagttgg tgaagatgaa gttgtatttt gtagtggatg tgattatgca
    10261 gccaatgtag aaagagcaga atcttgtaat ctagcatcac aaaaagaaga aatgaaagaa
    10321 ttagaagagg ttcatacacc aggagcagct acaataaaag agttagaaga atttttaaaa
    10381 acttctccag ataagtttgc taaaacttta gtatatgagg cagatggaaa aacagttgta
    10441 gtcgttgtaa gaggagatag agaagttaat gaaatcaaag tatctaatgc cataggttct
    10501 gttatagaat ttgctcttgc tacagatgat gtagtgagaa aagtaactaa tgcagaagta
    10561 ggatttgcag gtccaatagg tataaatgct gactatgtat ttatagataa agaaattgtt
    10621 gaacaaagaa acatagttgt tggggctaat aaaactgagt accatataaa aaatgctaac
    10681 tatggaagag attttgaagg aatagttgga gattttagaa atgttcaaga aggcgacaag
    10741 tgtatagtat gtggtaagcc acttgaaata gctagaggtg ttgaagtagg acatatattt
    10801 aaactaggaa ctaagtactc agaatctatg aatgcaaact tcatagacaa agatggaaaa
    10861 tcaaagccta tagtaatggg atgttatgga ataggtgttg aaagaacagc agcagctata
    10921 attgaacaac ataatgatga gaaaggtata atatggcctt tgtcagtagc tccataccat
    10981 gttgtgatag ttccagcaaa tatgaaaaat gaagaacaaa tatcaatagc tgaaaatata
    11041 tataatgatt tgcaagctat gggtgtagaa gttttattag atgacagaga tgaaagaata
    11101 ggtgtcaaat tcaatgattc agaattaata ggaataccta tgaggattac agtaggtaaa
    11161 aatattaatg aaggtaaagt agaatttaaa cttagacata aagaagataa agagataatt
    11221 gacatagaag aaataaatga aaaagtaaag gcagagttta tcaggaataa tgttaggtta
    11281 ggataataac ttaaataaga tattgtatag ataatcaaag ttgtacttta tagacaccat
    11341 tttatctata tataaggata taaaatggtg tttttttata aaacaaataa atgaggaggt
    11401 aattaaaatg gcaaaaaatg aaaaacaatt tgttgaagaa ataacaaaga tggaagatga
    11461 ttttccacaa tggtacacag atgtaataac taagactgat ttagttgatt atgcaccagt
    11521 aaaaggtttt atggttgtaa agccatatgg atatgcatta tgggaaaaga tgcaagaatt
    11581 tatggataaa aaatttaaag aaactggaca taaaaactgt tattttccat tattaatacc
    11641 agagagttta ttaaacaaag aagcagaaca cgttgaaggt tttgctccag aagttgcatg
    11701 ggtaacacat ggaggaaata aaaaattaga agaaagatta tgtgtaagac ctacttcaga
    11761 gactataata tgtacaatgt atgcgaaatg gttaaaatca tatagagaat taccatatct
    11821 ttataatcaa tggtgctcag ttgttagatg ggaaaaatca actagacctt tcttaagaac
    11881 atctgagttt ttatggcaag aaggtcatac gttacatgaa acagcagaag aagcacaaga
    11941 agaaacaata caacaattag aagtatataa agcgctatgt gaagaattac ttgcaatgcc
    12001 agttgtagct ggtcaaaaaa gtgaaagtga aaaatttgca ggtggtgaaa gaacatatac
    12061 tatagaagct atgatgcatg atggtaaagc attacaatca ggtacaagtc acttcctagg
    12121 tcaacacttt actaaggctt ttgatattac ttttgcagat agagaaggaa atttagctaa
    12181 tccatatcat acatcttggg gagcatctac aagacttata ggtggactta taatgactca
    12241 tagtgataac agaggtttag tattaccacc aagagtagct ccaattcaag ttgttatagt
    12301 tcctatagct gctaaaaaag gaaatgtaat ggagactgtt gataaaatat atgctgattt
    12361 aaaagctaag ggagtagctg tagaagttga tgatagagat aactatacaa ctggatggaa
    12421 atttaatgag tgggaaatga aaggtgttcc agtaagagtt gaaattggac caaaagatat
    12481 agaaaataat caagctatgg tatttagaag agatacttta gaaaaggatt ctatgccttt
    12541 agaaggatta gctgatgcta tatgtgattt atttgatgtt atacacaatg atatgtttga
    12601 aaaagcaaga aaacatagag aagacaatac atctatagtt gaaaatatgg atgagtttag
    12661 aaaagcttta gaagaaaagc caggattcat aaagactatg tggtgtggag atgcagaatg
    12721 tgaagctaag attaaggaag aaacaggagc aactataaga tgcttacctt ttgaacaaga
    12781 aaacttaggt cacaagtgtg tgtactgtgg aaaagaagca gatagtatgg tagtaatggc
    12841 aaaagcttac taaaatacat ttaaataaaa actggggtag aaagtattta atactttatc
    12901 ccagtttttt tgttttttat gagtaataaa atgtatttgt aactatgtca taagataagt
    12961 catagatact tatattaaaa agaatataat agagaactat ttttaataat tacatattaa
    13021 tgaaagcaaa atatttttgt tatatagatt ttcaataaag caaacaataa atataaatga
    13081 atattagtaa ggagtgcttt taatttttag aaaataaatg acttaaaaga aagatttatg
    13141 aaattaaaac taggattttc tgatataata aacatgtgta tttttaaata aaaaggatat
    13201 tattattaga tttgattaaa aataaacttg caaaacaatg tactagttta atattatagt
    13261 tatattgaat aaaatatcag gaggaagtaa aaatgaatgt aagagtaagg tttgcaccaa
    13321 gtccaacagg atttgtacat ataggtagtt tgagaactgc tttatataac tatttatttg
    13381 caaaaaaaat gggcggagaa tatatattaa gagtggaaga tacagaccaa agtagattag
    13441 tagaaggtgc tatagagaat atgcttaatg ctatgaaatg ggctggggtc aaccatagtg
    13501 aaggtgttat attagatgat agtggaaaag tagtacaaaa aggagaatat ggtccatata
    13561 ttcaatctca aagattagat atataccaag aatatataaa gcaactttta gatagtggaa
    13621 aagcttatta ctgtttctgt acaaaagaaa gactggatga agtaagagat gctcaaagag
    13681 cagcaggaga aacagctaaa tatgatggtc attgtaagaa tctttctaaa gaagaagtag
    13741 aagcgaatat aaaagcagga ataccatatg ttataagatt aagattgcca gagaatcata
    13801 caatcaagtt tacagactta gttagaggag atatggaatt taatactaat gacttagatg
    13861 accaagtatt aatgaaaaca gatggatttc caacttatca ttttgcagta gttgttgatg
    13921 actatttgat gaaaattaca catgttataa gaggagaaga atgggtatca tctactccaa
    13981 aacatgttta tttatatgaa gcttttggat gggaagcacc tgtatttgtg catttaccaa
    14041 atatacttaa taaagaaaag aaaaaattaa gtaaaagaca aggtgatgta gctgttgaag
    14101 actttaagaa aaaaggatat ttaccagagg gattagtaaa ctatgtagca ttagttggtt
    14161 ggtctcctga agataatcaa gaattgttta caatggagga attagaaaaa gccttctctg
    14221 tagaaagagt atctaaaagt ggtggagtat ttgatactga aaaattaaat tgggttaatc
    14281 agcattatat aaaagatggt gatgatgctt atctaacaga cttagcaata ccatttttaa
    14341 tagaagatgg atttataaca gaagaagaag ctactaataa atatgatttc ttaaagagta
    14401 tgatatcagt acttaaagag aaattacaat atgtaaaaga agttactgaa catgcaagta
    14461 tattttttgg agataaaata gaggtagaaa ctgaagaggg aagagagttc ttaagattag
    14521 aacatatacc tactttaata gatgctttga gagaaaaaat agaaaaaaca gaggtattaa
    14581 atgctgagtt tgttcaagct atgcttaaag aaatacaaaa agagtacaaa ataaaaggga
    14641 aaaatttatt tatgggctca agaataattt tgacagggca aatgcatggt ccagatttac
    14701 caaaagtaat ggaagtttta ggaaaagaaa cttgcttaaa tagaatagct tatgtaaaaa
    14761 ataatatatt atagtcataa aagtccgcta atttagaagc ggactttttt tgtgttagta
    14821 tttggtgaaa atatctgctt aagaaaatga aggtatttaa ggtaataaat ttaatatata
    14881 aaaagcaaag caaaaatata aataaaatat gaataataaa ctaataaaat attaagcata
    14941 gaagaaggga atctattatt ttagtttgat ttaattatta agggggtaat tactatgtat
    15001 agtaatattg atgatgttaa aaaggaatta aaggagctat gcttagaata tgttacaatt
    15061 ttagaaaaat taaaagatga aaaaatgatt acagaagaaa cttttgaaaa gtgctcttct
    15121 caaaaaaaaa tatttttaga agaacaatag aggatggtaa tagtattatt attatgtagt
    15181 ttgaaagata gtacgaaaaa tgatagtaat actattattt attgcaattt tcaactataa
    15241 tataatatat aatgttaaga agataattta taaaaataca gtgaggtgat gagtgtgaaa
    15301 gtttataata cattgactag aacaaaagaa gaatttgttc cattagaaga aggaaaggta
    15361 aaaatgtatg tgtgtggtcc aacagtttat aactatatac acataggtaa tgcaagacca
    15421 tttataatat ttgatactct aagaaggtat ttagagtata gaggatatga tgtaacttat
    15481 gtacaaaact ttacagatgt tgatgataag attataaata gaagtcatga ggagggaata
    15541 tctcctgaag aagttgcggc taagtatata aaagaatatt ttgtagactg tgatgggcta
    15601 ggaataaaaa gagctactgt tcatcctcag gttacagata atatacaaca gattatagag
    15661 tttataaaag agttggaaga taagggatat gcatatgcag taaatggtga tgtatatttt
    15721 gatacaaata agtttgaagg ttatggaaaa ctttcaggtc aaaaacaaga agatttagag
    15781 gcaggggcta gaattgaggt taatgaccaa aaaagacatc ctatggactt tgttctttgg
    15841 aaggcaaaaa aagaaggtga gccaggatgg gatagccctt ggggagaagg tagaccagga
    15901 tggcatatag aatgttctgt tatgtctaag agatatttag gtgaaacaat tgatatacat
    15961 gcaggtgggc aagatttaac attcccacat catgaaaatg aaatagctca aagtgaagct
    16021 agaagtggaa aaacattctc aaaatactgg atgcataatg gatatataaa tataaatgat
    16081 gaaaagatga gtaaatcaaa aggtaatttc tttacagtaa gagatatatc aaaattatat
    16141 gatttagaga tagtaagatt tttcatgtta tcagcacatt atagaaatcc agtaaacttt
    16201 agtgatgaga tgttaaatca agctaaagca ggtcttgaaa gactatataa tactaaagaa
    16261 aaattagaat ttacgctaag taatttagta gaatctccat taacagaaaa agaagtagag
    16321 cttgttaaag aattagatga ctttagacaa aaattcatag atgctatgga tgatgatgtt
    16381 aatacagcag acgcagtaag tgtaatattt gaattagcta agttaataaa ttctaatgta
    16441 gatgaaaatt cttctttgga gtttgctaaa aaatgcttag atgaatttaa tgaacttaca
    16501 ggagtattaa atattgtaaa taagaaaaag gatactgtat tagataaaga cattgaagaa
    16561 ttgatacaaa aaagaactga tgcaaagaaa aataaagagt ttcaattagc tgatgatatt
    16621 agacaacaat tactagatat gggaattgta ttagaagaca caagacaagg tgttaagtgg
    16681 aaaaggatat aaaaatggaa aaaactgaat tagttactat gtcaccatta gttttagcat
    16741 acttaggtga tacagtgtat gagacatata taagagaata tttaattaga cagaatacac
    16801 aaagaaaagt taatgatttg cataaactag ctataaagta tgtaaaagct aaagctcagg
    16861 caactataat tcatgaaatt gaaattgagt taactgaaga agagagtaaa atttataaaa
    16921 ggggaagaaa tcaaaagtca aatacttctc ctaagaatgc agatattata gactacaaac
    16981 atgcaactgg gtttgaggca ttagtgggat atttgtattt aaataatgaa atagagagac
    17041 ttcaatatat tatcaataaa ggaatcaaaa ttattgaaag agatatgtaa atattaaaga
    17101 gtgttgtctt aataataagt aataaggcaa cacttttttt ataactaatg tataataaat
    17161 atatagaaat tagtaatgag aaaggataaa aagttatgaa agtaaaatta atatcacata
    17221 cacctgaacc agaaaaagta atagctatgg cagctaagct atgttattca ccagttggga
    17281 cagacgaaat agaaaaagac ttaacagatg aaagtataga gaaattttta aatatgttat
    17341 taagtatagg tcatgggtca atattagaac atgcttcatt tacattttca atagaaggta
    17401 tttcaagggc ttgttcacat caaattgtta gacatcgtat agctagtttt tcacaacaaa
    17461 gtcaaagata tgttaaatta gaacaatttg agtacataat tcctccagaa atagaaaaaa
    17521 tagaaaaagc aaaagagtta tttatagact ccatgaaaaa agaccaagag aattatgata
    17581 agttagttga aatattgttt gaaaatcatt ataatgacct aataaaaaac ggtaaaaatg
    17641 agaaaacagc taaaagacaa gctgaaaaga aagcaataga agatgctaga tacgtatttc
    17701 caaatgcatg tgaaacaaaa atggtattta ctataaatgc tagaagtttg tttaatttct
    17761 ttgaacatag atgctgtgaa agagcacagt gggaaataag aaatttggct gtggagatgt
    17821 taaaagaggt taaaaaggtt gctcctatat tatttaaaaa aactggtcca agttgtgtaa
    17881 atggaagttg tccagaagga actatgactt gtggagatat agttcaagtt agagaaaaat
    17941 ttaaggcctt gtaggaggtg aataatttgg cgagtataga aggaagaaat ccggtaatag
    18001 aagcaataaa aagtgacaga gaaatagata aaatattaat tgcaaattca gctaaagaag
    18061 gttcaattaa gaaaataata ggaatggcta aagataagaa tataattatt cagtatgttg
    18121 ataaacataa gctagatgaa gtaagtacaa gtcattcaca tcaaggtgta atagcttatg
    18181 caagtgagta taagtattat gaattagatg agctaataga tttagcaaag aataaagatg
    18241 aagacccatt tttcattata ttagatgaga taacagaccc acataatttg ggttcaataa
    18301 taaggacagc agatgcagta ggagcacatg gagttatcat tccaaaaaga aggtctgtac
    18361 acataactcc tgtagttacc aaagcatctg ctggagcagt agaatatatg ccagtttgca
    18421 aggtaaccaa tatagttaat acaataaaaa gactcaaaga agaagggctg tggattgctg
    18481 cagctgatat ggatggagaa actttttata aacaaaatct tactggtcct cttggtgtag
    18541 taattggaag tgaaggtttt ggaatatcta gattggttaa acaaaactgt gactttatag
    18601 taaaaatgcc aatgataggt aatgttacat ctttaaatgc atctgtagca ggtggaatac
    18661 ttttatatga aatattcaga caaagactgg ataaaagtaa ataggcatga aaaaaaatat
    18721 aaatcattac ttaataatag atggctacaa tattataaat gcgtgggaca atttgaaaga
    18781 actggctaaa gaggacttag aagattctcg agaaaagctt attgatgaca ttatagaatt
    18841 ttctgagttt atgggttata agactataat agtttttgat gcttataatg ttaaaaattc
    18901 tcgagaaaaa gttgaaaaaa gaaaacacat aacaatagtt tatacaagag aacaccaaac
    18961 agcagatagt tatatagaga aatttataac ttctttatca aagtatgatg atgtaaaagt
    19021 agccactaat gactatgcag aacagcaaat gattttagga aaaggtgcta ctagaatgtc
    19081 tgcaagagaa ttaaagttag agttagatcg ttccaagaat aaaatgaagg aaaaaaatat
    19141 cggtttgagg aaaaaaattc agcgaaattg gttggaggaa aggttagata aagaaacatt
    19201 gtcgaaactt gagaacattc gtagaaagcg ttgaaattac taggttcttt agactataat
    19261 atacctataa atttgtttta tatatggggg agatttacat gttggtagca aaagaaaaaa
    19321 gttatgagtt agtggacaat tgtcagcagg atgagtataa cattgtatta agagcaagtg
    19381 aaggggataa aatagcacta gagtacatta ttactaaata cagaaatttt gtaaaggcaa
    19441 aagcaaagtc atatttttta attggagcag ataaagaaga tataatacaa gaaggaatga
    19501 taggccttta taaggcagtt agagattttg atggaagtaa gaccaattca tttaaatgtt
    19561 ttgcggagat atgtataaca agacaaataa taactgctat aaaaactgca acaagacaaa
    19621 aacatatacc tttaaattca tatgtttctt tgaataaacc aatatatgat gaagagtcag
    19681 acagaacact tttagatatt atagctacaa gtatagtaac agatccagaa gagcttataa
    19741 ttagtaaaga agagttaaaa aatatagaat caaaaatgaa cgaattgtta agtgacttgg
    19801 aattagaagt actagaatta tatttaaatg gaaagtcata ccagtttata gcggacaagc
    19861 ttaaaagaga tgtaaagtca atagataacg ctttacaaag ggttaagaga aaattagaaa
    19921 aacatcttga aaacagaaat gattaatagt aaaatactta ccataagctt attgtgagca
    19981 aaattaattt atatcgaaag ataagggagg aaattcaaaa tggctaaagc taaatacgaa
    20041 agaacaaaac ctcatgttaa tatagggaca ataggacacg tagaccacgg taaaactaca
    20101 ttaacagcag caataacaaa aacattatat gacagatatc aattaggaga agcagtagat
    20161 ttcgcaaaca tagataaagc tccagaagaa agagaaagag gaatcacaat atcaacagca
    20221 cacgttgagt atgaaacacc aaatagacac tatgcacacg ttgactgccc aggacatgct
    20281 gactacgtta agaacatgat aacaggagca gcacaaatgg acggagcaat attagtttgt
    20341 tcagcaacag atggaccaat gccacaaaca agagagcata tactattatc aagacaagtt
    20401 ggtgtaccat atatagtagt attcttaaac aaatgtgaca tggtagatga tgaagaatta
    20461 ttagagttag tagaaatgga agtaagagat ttattaacag aatatgattt cccaggagat
    20521 gacactccaa tagtaagagg ttcagcatta atggcattag aagatccaaa gagtgagtgg
    20581 ggagataaga tagtagaatt attcgagcaa atagatgagt atataccagc tccagagaga
    20641 gatacagata aaccattctt aatgccagta gaggacgtat tctcaatcac aggaagagga
    20701 acagttgcaa caggaagagt ggaaagagga gtact
//
LOCUS       DAEORZ010000064        20579 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEORZ010000064 DAEORZ010000000
VERSION     DAEORZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20579
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.064"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(20..667)
                     /locus_tag="KRH39_002099"
     CDS             complement(20..667)
                     /locus_tag="KRH39_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8511999.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLCDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            complement(660..1823)
                     /locus_tag="KRH39_002100"
     CDS             complement(660..1823)
                     /locus_tag="KRH39_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8512000.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILISYFLELILIFILCNYTKTFNSIYFVPLILDISFLIKEKYK
                     YILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLR
                     ISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLL
                     KDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNI
                     NVKLTISKNTWNLSRNQSIALYRLIQESLSNSSRHGKATEIRIFITFNTSNLIITISD
                     NGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            2007..2351
                     /locus_tag="KRH39_002101"
     CDS             2007..2351
                     /locus_tag="KRH39_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBG8512001.1"
                     /translation="MNNKEKGDFGEKVAINYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            2382..3983
                     /locus_tag="KRH39_002102"
     CDS             2382..3983
                     /locus_tag="KRH39_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBG8512002.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKVNNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFSKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            4002..5102
                     /gene="dprA"
                     /locus_tag="KRH39_002103"
     CDS             4002..5102
                     /gene="dprA"
                     /locus_tag="KRH39_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBG8512003.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            5135..7222
                     /gene="topA"
                     /locus_tag="KRH39_002104"
     CDS             5135..7222
                     /gene="topA"
                     /locus_tag="KRH39_002104"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBG8512004.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            7387..8172
                     /gene="codY"
                     /locus_tag="KRH39_002105"
     CDS             7387..8172
                     /gene="codY"
                     /locus_tag="KRH39_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBG8512005.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDAQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            8279..9544
                     /locus_tag="KRH39_002106"
     CDS             8279..9544
                     /locus_tag="KRH39_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBG8512006.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRSSTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            9575..10345
                     /locus_tag="KRH39_002107"
     CDS             9575..10345
                     /locus_tag="KRH39_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8512007.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYRNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNEKVKKIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            10454..10900
                     /locus_tag="KRH39_002108"
     CDS             10454..10900
                     /locus_tag="KRH39_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG8512008.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            10918..12111
                     /gene="nifS"
                     /locus_tag="KRH39_002109"
     CDS             10918..12111
                     /gene="nifS"
                     /locus_tag="KRH39_002109"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBG8512009.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            12165..12605
                     /gene="nifU"
                     /locus_tag="KRH39_002110"
     CDS             12165..12605
                     /gene="nifU"
                     /locus_tag="KRH39_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBG8512010.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGRTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            12898..13977
                     /gene="mnmA"
                     /locus_tag="KRH39_002111"
     CDS             12898..13977
                     /gene="mnmA"
                     /locus_tag="KRH39_002111"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBG8512011.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDDVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    14119..14357
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            14425..17064
                     /gene="alaS"
                     /locus_tag="KRH39_002112"
     CDS             14425..17064
                     /gene="alaS"
                     /locus_tag="KRH39_002112"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBG8512012.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAKEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDGETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            17089..17346
                     /locus_tag="KRH39_002113"
     CDS             17089..17346
                     /locus_tag="KRH39_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBG8512013.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            17356..19527
                     /locus_tag="KRH39_002114"
     CDS             17356..19527
                     /locus_tag="KRH39_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512014.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYALVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            19733..20158
                     /gene="ruvX"
                     /locus_tag="KRH39_002115"
     CDS             19733..20158
                     /gene="ruvX"
                     /locus_tag="KRH39_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBG8512015.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            20236..20523
                     /locus_tag="KRH39_002116"
     CDS             20236..20523
                     /locus_tag="KRH39_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBG8512016.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
BASE COUNT         8359 a         2157 c         3755 g         6308 t
ORIGIN      
        1 tcactttata tttttagatt tattctatta caattttatt tttgaaagca aagattgcta
       61 gttgagttct gtcacgaagt tctagtttag ctaaaatatt agttatgtta ttctttattg
      121 taccttcaga taagaatagt tcttgactta tttctttatt attaagtcca tttgcaatta
      181 gtctaattat agatatttct ttttctgtta aattgtacat gtcacaaaga tgctcttgtt
      241 taaccgtttt ttcagaggtc atgatttttt cagctacact tttatcaagc acaatattgc
      301 caaaaaatga agaacgaatc ccttcatgca atacttcatc agagctatct tttaaaaggt
      361 atcctgaagc accaaacttc atagcatctt ttatatattc atcatcatta aaagttgtta
      421 aaattaataa tgttatagaa ttattatatt cctttataag ccttgtacct agaaccccat
      481 cacatacttt cattcgtata tccataagaa ctatgtctac atcattcatt ttacaaaatt
      541 ctaaagcttc atatccatta gaagcatctc ctacaatttc taaatcatta taagatgata
      601 atataatttt aagacctttt ctaattaatt tttcgtcatc tactataaga actttaattt
      661 tattcaaaaa aatcaccccc tgaaaattta ggaaaagaag ctctaattgt gaaaccatta
      721 ttagaattat caaattcaac tttaccattt aattcatata ttctctcatt gatactatta
      781 agtccatttc ctttttttat attgccacat cctataccat tatcactaat agtaattata
      841 agatttgaag tattgaatgt aataaaaatt ctgatttcag ttgcctttcc atgcctagat
      901 gagttggaaa gggattcctg tatgagtcta tataaagcta tagattgatt tctggataag
      961 ttccaagtat tttttgaaat agttaattta acattaatat ttgtaagctt aataaagctt
     1021 ttaactaatt ctttgatttt aaataaactt tggtaatttt catattcaac aggttttaac
     1081 tcagatatag cccttcttac atcttgaaaa ctttcagtta caaattctct aagttcatgt
     1141 actaagtcat aaattagagg tttgtctttt aagagttttt ctatagcatt taattgtatt
     1201 attgttgtag aaagagaatg tcctacacta tcatgtattt ctcttgatat tcgatttctt
     1261 tcttttaaaa ttgctaattc ttctatagaa cttgcataag tttccaaatc agcattaact
     1321 ttttttaatt catctttaga aattctaagc ttatcataaa gattttgact gtataatttg
     1381 gataaattct cattttctat atatatagaa agtacagtta taattagcaa tatagagctt
     1441 gactccagtg ccaaatatat atttttatct aaactaataa taagagaaga tattataaca
     1501 aaagagaaca atatgtactt atatttttcc tttattaaaa atgatatatc taatattaaa
     1561 ggaacaaagt aaatcgaatt aaaagttttt gtataattac aaagtataaa aattaatata
     1621 agttctaaaa agtaagaaat aagtataaat ttattctcat ttgataaaga aaaaaatcta
     1681 acttgattat ttattataat tagaaataat attatcatag ttatgaccgg atacaatgta
     1741 ttttcaacat ttttcaaata gcttacaact aatataaata aagataaata tcgtataaaa
     1801 gcaactaatt tttctctgtt cattaaaggc actcctccaa aaaaataaac ttttcctatg
     1861 aaaactagaa taatatcata aaagaataaa gtcaatagaa gtatttatta atattgatgg
     1921 aaataaaatc aatccaaaaa cttgaatagt aaaattgtat actgaccata atttaaaagt
     1981 gaaaatttaa tgtattggag tgaataatga ataataaaga aaaaggtgat tttggagaaa
     2041 aagtagcaat taattatttg ctatcaaaag gagcaaagat actagaaaaa aattacagat
     2101 taaagatagg ggaaatagat ataatagcaa aaatggaaga tgaaattata tttgtggagg
     2161 tgaaatcaag gtctaacata aagtttggct acccttgtga atctgtaagc ttcaaaaaaa
     2221 gaaaaaagat aattggagtt gcaaattatt atattataaa aaataactta aataacaccc
     2281 ccataagatt tgatgttata gaggtatatt tattagaaaa aagaataaat cacataatga
     2341 atgcttttta aaaaagataa tgtttttgga aaggattaaa tatgttaagt attattaatt
     2401 caagcaactt agtaggaatc gacagctttt tagttaaggt tgaagtagat gtaagcaatg
     2461 gaatacctag ttttaatatt gttggtcttc caggcaaaga aattaaagaa gcaagagaaa
     2521 gagtaaagtc agcaattctt aatagtggat ataaatttcc aagcacgaga atagttgtaa
     2581 atttatcacc agcagatata aaaaaagaag gagctttttt agatttatct ataagtatag
     2641 gccttttaag agagttaata aaaaaagatg aaaattacat aagagaaagt atgtttatag
     2701 gagaattatc attagatggg aaaataagaa aagtaagagg tatattgccc ataattatgg
     2761 gtgcaaaaac tcagaacata aaacgtattt ttataccaat tgagaacata aaagaaagtc
     2821 tatttgtaga tgaaattgat ataattccta ttaaaagttt aaaagaatgt gtcgattttt
     2881 taaacgaaga gataaaagtc gataaagtaa ggataatgtc ttttttggat gataagtcaa
     2941 gaaaagagaa cgaagaatta gaaaaagata atagttatat agattgcaaa tatactaagg
     3001 ttaataatga agaaagtaaa tatgatgaag actttaaaga tgtaaaagga aattattttg
     3061 taaagaggag tgcagaaatt gctgctgctg gaaatcataa tatgtttatg attggacctc
     3121 caggctcagg aaaaactatg attgcaaaaa gagtaaggac aattttacca gatataagtg
     3181 tagaagaaat gatagaagta agtaaggtct atagtatatt gggtatgata aatgaaacta
     3241 aaggaataat tgataaaaga ccttttagag cacctcatca tacaactaca aagcaatctt
     3301 taattggagg aggaatggat gcaagacctg gagaaattgc tttagcacat agaggtatct
     3361 tatttttaga tgagatagcc gaatttgata gaaaaatact tgagacttta agacaaccta
     3421 tagaagatgg atatgtaaat atatccagag taaagtacag tgcaaaatat ccttgtagag
     3481 tactgttagt tgcagctatg aatccatgtc catgtggtta ttatatgtca gagacagagt
     3541 gtagatgtag aagtaacgaa attgatagat acataaataa aatatcaggt ccattgttag
     3601 ataggtttga tatttttgta gaagttaatt ctataaaata ttcagatttt aattctttaa
     3661 aacaagaaga aagctctcaa aaaataaaga gaagagtaga aaatgctaga aaaatacaga
     3721 taaatagatt taagaaagat aatataaaaa ataatagtga aattaaagct tacaatttat
     3781 ttaaatattg taaactggaa aaagaagctt caaaaacggc tgagatgata tttagtaaat
     3841 acaacctaag cagtagaagt tatacaaaac ttttaaaaat ggcaagaaca atagcagatt
     3901 tagaagaaag agatttgata aattcacagt gtattataga agctttttca tttagaaagg
     3961 cttattatag ttattttaaa tagttttttg aggtggattt tatggaaaaa agagatactt
     4021 atttatggtt aaaatcaatt ggtggaataa caacaaaaac aattgaaata atagaaaatg
     4081 aaatagtaaa tattgaagat atatttgatt tttctgaaaa agaaatatat aatttaaaaa
     4141 atataagctt gaatattaga aaaaatatag taaaatatag aggtcacgct tatttagaaa
     4201 atataaaaga actactttac aaaaaagcga ttaaatatat ttgtaaatat gataaagaat
     4261 atcctgaaaa tttaaagaat atatacaacg ctccaaagtt attattctac aaaggtgaca
     4321 taggcttagt taataataat tttaatatag ctatagtagg ttcaagaaaa cctacagcct
     4381 atggaattaa ttgtgcaaaa acaataagtt gtcaactatc ccaatatgga gttaatatag
     4441 taagtggatt agctatagga atagatgcat actcgcacat aggttgtatg agtggaaaat
     4501 ctaaaactat agcagtactt ggttcaggag tagacaatcc tcttccaaaa caaaatttac
     4561 atttatcaaa taaaatatta gaaaatgggg gattattatt atcagagtat aatattaact
     4621 cgacagtagc cccgtaccat ttttctaata gaaatagaat tatcagtggt ttaagtgatg
     4681 gagtagttgt tgtagaagca gcaataaaaa gtggtgcact aatcacagta gattttgctc
     4741 ttgaacatgg aaaaaatgtt tttgcaatac ctggtaatat aaattctcaa atgagtagag
     4801 gttgtcataa aattataaaa gaaggtgcaa aattaattga aaatatagat gatattttaa
     4861 atgaatataa catatttaat attattgata aaaaaataaa tcaaaaatat gataatataa
     4921 gtctaaatgc caagtctaag caaataattg aagctataaa aagagaagga aacttgcata
     4981 tagatagtat ttgtgattat actggtatag aaataaagta tgtaaattca ataatcaatg
     5041 aattagtact aaatgagtta gtagtagaaa tgaataataa aacgtacagt ttaaatgtat
     5101 aaattaagat taggtagtgg gggtgtatgg ttttatggcc aaaacattag tcatcgtaga
     5161 atcgcctgca aaagctaaaa ctatagaaaa atttttaggg aaaagtcatt acacagttaa
     5221 agcatcagtt ggtcatgtca gagatttacc taaaagtaaa ttaggtgtag atatagaaaa
     5281 taattttgaa cctcaatata taaatataag aggaaaaggc gatgtaataa aagaattaaa
     5341 aaaagaagct aaaaaatcta aacaagtata tctagcaact gaccctgata gagaaggtga
     5401 agctatatca tggcatttag ctcatatact aaatttagat gaatctgagg attgtagaat
     5461 agagtttaat gagataacta aggatgcaat taaaaaagca attaaacatc ctagaaatat
     5521 tgatataaat ttggttgatg cacaacaggc aagaagagtg ctagacagac ttttaggtta
     5581 tcaaataagt cctatattat ggcaaaaggt tagaaaaggt cttagtgcag gtagagtgca
     5641 atctgttaca acaaagttaa tttgtgacag agaaaaagaa ataaaggcat ttattccaaa
     5701 agaatactgg acaatagatg ttgaggctaa gacagtaaaa gacaaagata taactttgaa
     5761 attttatggt aaaaatgatg aaaagattga attagaaaat gaagaagctg taaatgaaat
     5821 actaaaagaa atggaaggta aagatttaaa ggttgagaag atagaatcta aatcaagacg
     5881 taaatcagct ccaaaaccat ttacaacaag tatgcttcaa caagaaggag caaataagtt
     5941 attttttact actaaaaaaa caatgatgat agcacaagaa ttatatgaag gtatcgacat
     6001 agaaggtgaa ggtactgttg gtcttatatc atatataaga actgactcta aaagaatatc
     6061 tgatgaagca aaagaaaaag ctaagggcta tatcgtaaac aatttaggag aaaactacta
     6121 taaaaacact ggtgataaaa aagaaaaaaa ggatactaaa aaggttcaag atgcccatga
     6181 ggcaataaga ccaacctcag ttgatagaac tcctgagagt ataaaagcct cattgagcaa
     6241 agaccagtac aagttataca atttaatttg gagaagattt gtagctagtc aaatggaaga
     6301 ttcagtgttt gatatactaa atgtagaatg taaaataggc gatattgtat ttaaggcaac
     6361 tggttcaaaa atgaaatttg atggttatac caaggtatat aatttttcag aaagagaaga
     6421 caaaatttta ccaagtatag aaaaaggaga tatattaaat atagaaaata tattgccaga
     6481 ccaacatttt acacaaccac cagcaagata tacagaagct agtttagtaa aaacactaga
     6541 agaactaggt ataggtagac caagtacgta tgctccaaca atagctacta tactgaatag
     6601 agagtatgtt gaaaagcaag gaacaagtct atgtccaaca gaacttggaa taatagttac
     6661 tgatattttg gaaaataatt tccataaatt tatagatgta gattttactg ctgatatgga
     6721 aagtaaactt gatgttatag aagaaggcaa tacagattgg aaaaagattg tatcagaatc
     6781 atatgcacca cttaaagagg ctattgaaga agctcttgaa aatatagaaa aagtaaatat
     6841 ggatgaagaa actgatgaga tttgcgagaa ctgtgggtct aatatggtta tcaaatatgg
     6901 tagatttgga aagtttatgg cttgtaaaaa ctatcccgat tgtaaaacaa ctagaccaat
     6961 tatgaataaa attggtgtta aatgtccaaa atgtgaagaa ggagatatta tacttagaaa
     7021 atctaaaaga ggtaaagctt tttatgggtg ttctaattat ccaaactgtg actttgtagc
     7081 ttggaataag ccaacaggag agttttgtga gaagtgtggt tcatatatgg ttgaaaaagt
     7141 gactaaatct gaaacaaaaa cagtgtgttc taataaagaa tgtaataaag agacaaaaga
     7201 gcataatgac ggaaaagagt gaaaattgaa aaaaatcaca taattctctt gaaaaaggtt
     7261 ccaatctatg ataatctttc aagtgtaaac aactaataga aaaataaatt ttcattaaat
     7321 gtgaaaataa tattagaaaa tataaaaggt tattaaaagg cctaaatttc atgaggagat
     7381 gattaaatgg caagtgaagt gttacaaaaa acaaggaaaa taaataaaac attacaaaca
     7441 agtggtggaa gtagtgtctc ttttgattta ctggccggag cattgggaga tgttttaagt
     7501 tctaatgttt atgtagtaag tgcaaaaggt aaagtgctag gtcttcattt aaatgatgct
     7561 caagacagtt cagttataga agatgagtat actaagcaaa agaaattttc agatgaatat
     7621 actcaaaatg tgttaaaaat cgatgaaaca ttagaaaatt taaatggtga gaagatatta
     7681 gaaatctttc ctgaagaaca tggaagatta caaaaatata ctacagtagt tccaatatta
     7741 ggaagcggtc aaagattagg aacattggta ctttcaagat attcaaattc attcaatgat
     7801 gatgatttag taatagctga atacagtgca actgttgttg gtcttgaaat attaagagca
     7861 ataggtgaag aattagaaga agaaatgaga aagaaagctg tagttcaaat ggcaataggc
     7921 actctgtcct actccgagct tgaagcagtt gaacatattt ttgctgaatt ggatggaaaa
     7981 gaaggtctac ttgtagcaag taagatagct gatagagttg gtataactag gtctgtaata
     8041 gtaaatgcac ttagaaaatt tgagagtgca ggtgtgatag aatcaagatc attaggtatg
     8101 aaaggtactc atataagaat acttaatgac aaacttacag atgaattaaa aaaattaaaa
     8161 aacaatcaat aatctatatt ttataggttt agattagaaa aggtatctat ttcaaataga
     8221 tacctttttt agacgaatta ttaaggtaat gataagtata ttaaaaagga gtgagaaaat
     8281 gcctttagca gataaaataa gaccaaaaac aatgaatgat gtaataggtc aaagtcatat
     8341 aataggtaat ggaaaaatat tgtctaagat attacaatct aattttttac caaatatgat
     8401 attttttggt cctcctggtg ttggtaaaac tacagtagcg gaaataatag cagaaagaag
     8461 caataaaagc ttctataaaa taaatgctac taattcatct ttagaagata taaaaaaggt
     8521 gatatcagag cttggaagta taaataatgt aaatggagtc ctattatata tagatgaaat
     8581 acaaagtttt aataaaaaac agcaacaatc aatattagaa tttatggaaa atgggagtat
     8641 aactttgatt gctagcacta cagaaaatcc atatcattat gtatataaag ctcttttaag
     8701 taggtctact gtatttgaat ttaaaccttt agaaaaactt gatatagaaa aaggactgaa
     8761 aagagctgtt gaagtattaa atgaagatag ctatatggat attgagtgta ataatgatgc
     8821 tattgaagat atagcaattc tttctgatgg agatatgaga agagccttaa atattttgga
     8881 agtggtagtc tactcaacta aaccaaataa ggataatata acttatatag attcagatgt
     8941 aataaggtca tctacattta ataaaattat aaactatgac aaaaatggtg atagtcatta
     9001 tgatatttta agtgcatttc aaaaatctat aagaggaagt gacccacagg catctataca
     9061 ctatctagca agacttataa aaggtgggga tttgataagt atatgtaggc gacttttagt
     9121 aatagctagt gaagatatag gtttagctta tccaaatgct attgtaattg taaaagcatg
     9181 tgtagactca gctatgcaac ttggatttcc agaagcgaaa ataccattag cagaagctac
     9241 tatccttttg gctacatctc caaaatctaa ttctgcatgt atagcaataa tgaaagctat
     9301 ggatgaactg gataaagagt ttgttaagga tataccaaat agcataaagg atgctcacta
     9361 ttctggttct aaagaaatag gtaggggttg tggatacaaa tatcctcata attttaaaaa
     9421 tcattatgta aagcaacagt atttaccaga tagcataaaa gatagtattt attacatacc
     9481 acaagaaaat aaaactgaga aaaatataaa aaaatattta gaatatctaa atagtgattt
     9541 ttagactgaa ctttatgtat cgggggtaga tattatgatt ataaaactta actcaactta
     9601 tcacagtaga gttatgaaat acttaaaaaa agaacctgag tacaatttgt ttataatagg
     9661 ggatattgaa agatatggat atagaaataa ttttttaaat atatgggcag atgtaggtga
     9721 acatggtgaa ataaaggcaa tattacttaa atattttgaa tttatgatgt tttattctga
     9781 tggagagtat gatgtagaag gtttttataa tctacttcgt aatactaatt acgaagaaat
     9841 atctgggaaa atatgtgctg tagatgcttt agcaaagaga ttaggactta ataatttaaa
     9901 ggtagttgat ttttgcaaac ttcaaactaa aaagtttttg atagataata attgtaatga
     9961 aaaggttaaa aaaattaggc ttggaaatct aaaaaagaca gtgaaacttt atgacttaat
    10021 agatgaattt cacagtacaa ctttagagaa tttaaaaaat ggactaagga caggtagagg
    10081 atattgtata gagattaaca aacaggtggt gtcaatggca aagagtactt cagaaaatag
    10141 aactcatgca atgataattg gagtaggaac acatcctaaa tatagagcta aaggacttgc
    10201 cacaaaatgc ctaattaaac tatgttcaga gttactgaga gaaaacaaaa ttccttgttt
    10261 gttttatgat aatgaagagg ctggaaagat atataaaaaa ctaggatttg aaaacattgg
    10321 aaaatgggga atttattcaa aataaaaaat taaaattaat cttgacaaaa ttagtagggt
    10381 ataataaaat tatcttgaaa gtaattccta ctaaaacagt atgaattaga tttctatata
    10441 gaggtgataa tatatgaagt tgtctactaa aggtagatat ggactaaaag caatgtttga
    10501 attagcatta aatcaagaca atggaccagt atcattaaaa tttattgcta agaagcaaaa
    10561 aatatcagat cagtacttag aacaaatatt ttcatcttta aaaaaatcgg gtctagtaaa
    10621 aagtgtgaga ggtgctcaag gaggttattt gctttcaaag aatgctgaag acataacagt
    10681 aggagatata cttgtagtat tagaaggacc agttgcatta tctgactgtg tactagatga
    10741 agatgtatgt gaaaactcaa acatgtgtgt aacaaaaata gtttgggaaa agatgaaaaa
    10801 gggtatagaa gatgttatag attcaattac tcttaaagat atgataaatg actataacaa
    10861 aaataaatta gaaaatgata taacaaatat taaaaaatag atttggagat gaatgtaatg
    10921 gaaaaaagaa gattgtatat ggattattct gcaacaacac ctattaaaaa agaagtattg
    10981 gatgcaatga tgccatacct aacagattat tttggtaatg catctagttt tcacacattc
    11041 ggaagagaag caaaagatgc tttggataaa gcaagagaac aagtagcagc ccttataaat
    11101 gctgaaccta gtgaaatata ttttacagca ggtggttcag aaagtgataa ctggacacta
    11161 gagggtgtag cttatgctaa taaaaacaaa ggtaatcata taataacttc aaaaatagag
    11221 caccatgcaa tacttcatac atgtgaatac ttagcaaaac atcatggatt tgaaataact
    11281 tatttagatg ttgatagcga aggaaaagta gacttgaaac aactagaaga ttcaataaaa
    11341 gatactacaa tacttataag cataatgttt gcaaataatg aaataggaac tatacagcct
    11401 attaaagaaa tatccgaaat agctaaaaaa cataagatat tattccacac tgatgcagta
    11461 caagctacag gtaatattcc agtagatgtt aaagagttag gaatagattt aatgagtatg
    11521 tcttcacata aaatatatgg accaaaaggt gtaggagctt tatacataag aaaaggtgta
    11581 agacttcata actttgtcca tggtggagca caagaaaaaa gcaaaagagc tggtacagaa
    11641 aatatccctg caatagttgg atatggaaaa gcagcagaat tagctaagga aaatatgcaa
    11701 aatcatgtag aaactttaac tcgtcttaga aataagttaa tagatggtgt gttagaaaga
    11761 ataccttata cgagagtaaa tggtagctta gaaaatagat taccaggaaa tgcaaacttt
    11821 gcattccaat ttatagaagg tgaaggaata cttttattat tagatatgtt aggaattgct
    11881 ggttcaagtg gttctgcatg tacttctggt tcattagacc cttcacatgt acttcttgca
    11941 ataggtttac cacatgaaat agcacatggt tcattaagac ttacagttgg agattttaca
    12001 acagatgatg atatagatta tatactagag aacttaccaa aagtaataga aagacttaga
    12061 agcatgtcac cactttatga tgatgcaaag aaacaaggtt tagtaaaata gacagaaaga
    12121 taaagcataa aaaatagaca taaattgagt aggaggaaaa gataatgcaa tatagtgata
    12181 aagttatgga acattttatg aatccaagaa atatgggaga aattgataat gcaagtggtg
    12241 taggtgaagt tggaaaccct acatgtggag atataatgaa gatattttta gacatagatg
    12301 gagatgtaat aaaggatgtt aagtttaaaa catttggctg tggttcagct atagcaagtt
    12361 cttctatggc aactgaaatg ataaaaggaa gaactataaa agatgcattg gagcttacaa
    12421 ataaagctgt tgcagaagct ttggatggtt taccaccagt aaagatgcat tgttcagttt
    12481 tagctgaaca agctgtaaaa gcagctctta tagattatgc tcaaaagaac aatatccata
    12541 taccagaatt ggatggatat gttatagatg atgctcatga ccatgatgtt gaagaagaag
    12601 aataaaaata agtgattgtg tgtgttatga gaagtttaca atgattaaaa agaagtacta
    12661 atgatataat taggacataa aaaatagctg tttttaaaat ggctattttt tattgcctaa
    12721 attaagtatt ttaacttgat ttagaggtta attagttaac aaaataatcc ataagattca
    12781 aataataaag atgattattt ttttagaaaa tagaacaagc tatattttaa ataagtaaaa
    12841 tgcttgctct atatggtata ataataagta atgcaaaaga tgaggtgaaa tttgtttatg
    12901 aataaaaaag taatgatagg tatgagtgga ggagttgata gctctgtagc tgcatatctt
    12961 ttaaaacaac aaggatatga tgtaattggt gttactatga agttgtggca agatgatgac
    13021 gatgttgaaa ttgaaggtgg ttgttgctct ttatctgctg ttgaagatgc tagaagggta
    13081 gcaaataaaa taggtatacc attttatgtt ttaaatttta gagaagtgtt taaagaaaaa
    13141 gtaatagatt attttataga tgaatatttg gaaggaaaaa ctcctaatcc ctgtatagct
    13201 tgtaataaac atataaaatt tgatgatttt tataagaagg ctagacaaat aggttgtgat
    13261 tacgtagcta caggtcacta tgcaaagata gaaaaggatg aaagtacagg aagatatttg
    13321 ctaaaaaaat ctgttacaga taaaaaagac caaacttatg cactttataa tcttacacaa
    13381 gagcaactag agcatacttt attaccaata ggtgactatg aaaaagatag agtaagagaa
    13441 attgctaagg aaatgggtat ggcagtacac aataagcctg atagtcaaga aatttgtttt
    13501 gttaaagata atgactatgc aaattatgta aaaaaacatt ctaaaaagcg tattgaagaa
    13561 ggtttttttg tagatactaa aggcaatata cttggaaaac ataagggtat attatattat
    13621 actataggtc aaagaaaagg gcttggaatt acttttggaa aaccaatgtt tgtaatagac
    13681 ataaatccaa taaataatac catagttctt ggagataatg aagatttatt taaaaaggaa
    13741 cttattgcta aagatgttaa ctttatatca atagatacgt tagaagaacc attaagagtt
    13801 caagccaaaa taagatattc agcaaagcca tcaccagcta ctattcatag agtaggagaa
    13861 gatactataa aaatagtttt tgatgaagct caaagagcta ttactaaagg tcaatctgta
    13921 gtaatgtatg atggagatat tgtagtaggt ggaggtataa tcgagaaaag tttataaaaa
    13981 catctaaaaa attaaaaaaa taaacaataa agtatattaa gagtaaataa agtttataaa
    14041 ataaatttat tgtatatgaa aatatagtat tgattaattt tgtaaaattt agtaatataa
    14101 ttagtatatt attaaaaaac aaatacagtg ataaggaata gtaaaatatt tgatgtctta
    14161 cagagagagg gaaatgctgg aagcccttag aatatgatat ttgaagcagc cttttagtag
    14221 caatcttgaa caatttatct tttataaaga agatttaagt agagattgac ggtagttacg
    14281 ttatagctaa ggttttccga agatatcata ctttgtatga taattagggt ggtaccgcga
    14341 ttgtcctcgc ccctataaat ttatttatag ggacgaggtt ttttatttaa acgaaaaaat
    14401 aaaatttatt aggaggaaca tgatatggaa aagatgggat taaatgaaat aagaagtaag
    14461 tttttagaat tttttgagtc aaaaggtcat tatgtagcaa atagttattc tttagtacca
    14521 aataatgata agagtttgtt acttataaac tcaggtatgg cacctttgaa aaactacttt
    14581 tcaggtgtag aagtacctcc aagtgtgaga atgtgtacat ctcaaaaatg tataagaaca
    14641 ggagatatag aaaatgtagg tataactgca agacatgcta cattctttga aatgatgggt
    14701 aacttctcat ttggagatta ctttaaaaga gagtcaatca aatggggatg ggaatttgtt
    14761 acagaatggc taaatatacc agaagataag atatgggtga ctgtatatga agaagatgat
    14821 gattcttatg atatatgggc aaaagaaatg aatttcccag aagagagaat ggttagactt
    14881 ggaaaagatg ataatttttg ggagataggt acaggtcctt gtggtccttg ctcagaaata
    14941 tattttgata gaggagaaga atatggatgt gacaatccag actgtaaacc aggctgtgat
    15001 tgtgataggt atttagaatt ttggaaccat gtatttactc aatttgatag agatgaagaa
    15061 ggaaattata gtctattaga aaacaagaat atagatacag gtatgggtct tgaaagaatg
    15121 ggatgtatta tgcaaggcgt agacactatc tttgaagttg atacaattaa atctatacta
    15181 gaagcagttg agaagttaac aggagtaaaa tatggagaga atccaaaaaa tgatatatca
    15241 ataagaataa taactgacca cataagagct gtaacattct tagttagtga tggagtgctt
    15301 ccatcaaatg aaggcagagg atatgttctt agaagacttc ttagacgtgc tgctcgtcat
    15361 ggtaagttat taggagtaaa agaattattt ttacagaaac taatagatga agttataaaa
    15421 gtaaatgata aagcatatcc agttttagtt gaaaaagaaa gttatattaa aaaagtagtt
    15481 ggaatagaag aagaaaaatt caatgaaact atagaccaag gaacagaaat attgaactct
    15541 tatatagaag tattaaaaaa tgaaggaaag actgtcctaa gtggtcaaga agcttttaaa
    15601 ctatatgata cttatggctt ccctatagac ttaactaaag aaatattaga agaagagcac
    15661 ctatctgtag atgaagaagc ttttaatgaa gaaatggaaa agcaaaaaga aagagctaga
    15721 aatgctagag gaaatatgga tggagaaagt tggaaggaag accctctatc aaaattagaa
    15781 tctacagttg acagtacatt taatgggtat agtgaaattt atggtgaagg aactattgaa
    15841 gctatagtta aagatgatga gctagttcaa agtgcaaaag aaggagataa ggtatctata
    15901 gtactagata acactacttt ctatcctgaa ggtggaggac aagttggaga ctgtggattg
    15961 ataactaatg aaaacttagt tttagaagta ttaaatacta aaaaaggtgc taataatagt
    16021 ataaaacata taggtataat aaaatcagga agaataagta atggggataa agtaaagaca
    16081 ctagtagatg gagaaactag aatgtcagct gcaagaaatc acagtgctac acatttactt
    16141 cataaggcat taagagaagt attaggagaa catgttaatc aagcaggctc tttagtaact
    16201 ccagaaagac ttagatttga tattactcac tttgaagcga tatcaaatga agaattaaaa
    16261 gtaatagaag aaaaagtaaa taatgttatt ttatcatctt tagacataaa atgtgatatt
    16321 atgaatataa aggaagctaa agaaaaagga gctacagcac tatttggtga aaaatatggt
    16381 gatgaggtaa gagtagtttc tatgggagat tattctacag aactttgtgg aggaactcac
    16441 ttgacaaata cttctcaagt aggaatgttc aaaatattat ctgaaggtgg agtagcagca
    16501 ggtgtaagaa gaatagaagc aataacagga aaagctgttt atgaatacct aaaagaaaga
    16561 gatggaatta tctctgaagt ttgtgtaaac ttaaaatcta aagaagataa tttaattcaa
    16621 agaataagtt ctttattaga agaaaataaa aacttatcta aagaattgca tgatatgaaa
    16681 gctaagatga gtttacaatc agttgattct atttttgatt ctaaagttga agtaaatgga
    16741 gttaatttga taactaataa atttgaaggt atggatatgg atacactaag agaaacagca
    16801 gataatctaa gagataagct tggttctggt gtagtagtac ttgcgaatgt agtagatgat
    16861 aaagttaact ttgtagttac tgccactaaa gatgtattag ataagggaat acattcaggt
    16921 aatattgtta gagaagttgc aaaaatagct ggaggaaaag gtggaggaag acctaatatg
    16981 gcacaagcag gagctagtga tgtatctaag gttgaccaag ctttaagcta tgcaagtgag
    17041 gttattaaaa cacaagttaa ataacattca ctcaaggagg tttttagttt ggaaaaagat
    17101 ttagactaca ctatgaaatt tgaaggaata ccagaagata ggatgagtgt aggagatact
    17161 attgattttg tatataaagc attagttgaa aaaggatata accctataaa tcaaattata
    17221 gggtatctcc tttctggtga ttctagctat ataactagtc ataaaaatgc aagagctata
    17281 attaaaaaat ttgaaagaga tgagatactt gaagaagtaa tcacgcacta tctgaataga
    17341 aagtaggtca aataaatgag aaaaaagatt atgtcgttgt ttgcaatagt cattatgatt
    17401 tttgtatcaa ctgctagcta tggttttgct gaagaaaaag gcaaggtcat atttattgat
    17461 atgaatagga ccaatcttgg aagtatgatg aatattccaa tcttgaaaaa tgagatagaa
    17521 aagagaggct atgtagctct catgaatata agaggggatc aaggtactga tgatagaagg
    17581 tcttatgcaa gtatgggtgc aggaacaaga gctagtgtaa ctactgaaga ctatatcaat
    17641 tttcaaaaag tagaccaaga tactgctaaa gcttttgaag tcgcaacagg tcaaaaagct
    17701 aaagaaataa atgatttagg cattaataga tctataaatg aaaatcttga gtatggagaa
    17761 tatggagcaa atttagggtc attaggaaaa actttatcag aaaatggttt aaagacatct
    17821 gtaattggaa atgctgatat aattgaaaat aatcaattag ttaaaaacag aaacttatgt
    17881 ttagtagcta tggaccaata tggaaggatt gattcaggta atattgataa tattaataag
    17941 aaagattcta aaatgccatt tggaatatct acagattatg ataaattact agatgaaact
    18001 aaaaaatcct ataaaaatag tgatgctata tttgtagaac ttggagatac atatagatta
    18061 gatatgtata gattaaatct aaatgataat acttatgata caatgaagaa aaatattgta
    18121 aaaaatataa ataaatatct tgaacaagta ttttctatgg ttggagaaaa tgacactgtt
    18181 tatatagcaa gtgcatttcc aagtgaccta gattataaga ataagagaag actttctcca
    18241 ataataaaat ttagtggaga agggaaaggt ctactggaat ctgccacaac aagaaaggct
    18301 ggaattgttg ccaatttaga tgttggagta gatatactag gtacatttgg tcttgaaaat
    18361 aaagagatgg taggtagaag ttatgcttta gtaaataaag aggacaatat tagctatcta
    18421 aaatcacttt atgaaaggat agtgtctata tcttcaataa ggtcaactat tataaatggc
    18481 tttgtaggtg taatatctgc ttcttgggta attgccatgt ttgcgatttt ctttagaaat
    18541 tatatgccac ataaggagcg tgtattcaaa gtacttaagg agtttataaa attaggcata
    18601 ataatgccac tatcatttat gttagctcct ttgatgaatt ttaaaactcc aatttcaata
    18661 tctttgggaa tatttgcaac tacgctaata ttatacttgt taggtagaaa attatttaag
    18721 gacaatgata taaagcacat gggatttttt gcaacactta ctattttagt catagttata
    18781 gattctgtat ttggaacata cttaatgcaa aataacataa tgagttatga tgctgtcata
    18841 ggagctaggt actatggtgt aggaaatgaa tatgaaggtg taactatagc aagtgcagta
    18901 tttggcttag cagtattatt acattataag aaggtatcta aattacttgt tgtaattttt
    18961 tcattaataa tacttataac tagtgcatat ccttcaatgg gtgcaaatgt tggtggagct
    19021 atatcagaat gtgtagcata tttgttgttt ataatgctta tatatgacat taaaatagat
    19081 tttaagaaag ctgtattatt agctgtttca gctgtagttg tcgtttttgc atttgcagct
    19141 ttagatatac tttcaggtag tgaatctcat ttaggtatgt ttgtacagag aattttccta
    19201 aatggaccag gagagatatt acaaacattt ggaagaaaaa ttcaaatgaa tatgcaacta
    19261 gcacagacaa gtgtatgggt aaatatttta cttgtaggaa ttggagtaat tgcagtgctt
    19321 atatttaaac catctagaca ctttagaaag ataatgaata attttccaat cttgtttaaa
    19381 gggtttattg catctatggt tggttgtata attactcttt tagttaatga ttctggaata
    19441 gttgctgcat ctacagcatc tatatatata cttataccac taataataat aagtataaat
    19501 atgataatat ttgaaaaaaa taactaaaaa aatttttact gttcatatag gatttaaata
    19561 aaaaatgcaa tatgattatt aatgtaaact ggatgcttac ataaaaaatc atattgcaat
    19621 ttttatgatt gattatgtaa aattctatga agcagtaagc ctgtgacctt agttatgaga
    19681 ggttcagaat atgtatttaa tctatagaat ataaatagat aaaggaggaa ctatgttaga
    19741 tggaagaata atgggtcttg atgttggtga taaaactatt ggagttgctg tgagtgattt
    19801 gatgggatta actgcacaag gtgtaaaaac aataaaaaga gttggcaaga aaaaagatat
    19861 tgaagagcta aaagcaataa taaaagaaaa acaagtaaat aaaattgtat caggattacc
    19921 aaaaaatatg aatggaactt taggtccaca aggagaaaaa gtaatcaagt tttgtgaatt
    19981 ggtaaaagca gaaacaggta ttgatgtaga gttttgggat gagaggcttt ctacagtagc
    20041 ggcagagagg tctttattag aagcagatgt tagtagacaa aaaagaaaaa aggttataga
    20101 tatgttggca gcagtaataa ttttgcaagg atatttagat tttaaaataa attcataaaa
    20161 gaactataaa attgaatact tatgatatat aatataaata ataaaaataa aaataaattt
    20221 gaggtgaatg acattatgga agaaaatata ataaatttaa tagacgaaaa tggcgttgaa
    20281 agccaatttg aaataatatt aacattagaa gcagaaggaa aagagtatgc aatattaatg
    20341 cctttagatg atgaagaagc tgaagaagct ttaatcttta gaattgatga ggatgaagaa
    20401 ggtgaaatct taataccttt ggaaagtgat gaagagtatg aaactgttgt agctgtgtat
    20461 actgctataa tggaagaaga aggtttaaat tacgacgaag atgaatcaaa tggacttaac
    20521 tagataagta attaagtttg tatataaaaa tcagatattt attatatttg ctttaatta
//
LOCUS       DAEORZ010000065        20300 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEORZ010000065 DAEORZ010000000
VERSION     DAEORZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.065"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..1542
                     /locus_tag="KRH39_002117"
     CDS             <1..1542
                     /locus_tag="KRH39_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8512017.1"
                     /translation="YLRKTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGYVS
                     IDGIISTPLATTYNAPILLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQI
                     KSTVNASQTRLNGSNRYETSLLIAKEIDKNHDVEKIYITNANGGEVDALTIAAKAGQD
                     KQPIILTDKDSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRV
                     YGADRHETNANVIKKFYTDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYV
                     SAYHKDNLEAKSANKVYKIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPG
                     HGGSAPGNSSGGMIEKDYNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNS
                     LKPDLFTSIHYNGSTNKQGHGVEVFYKLKDKNGGITKTVATNILNRILEKFKLTNRGI
                     KTRVLPSDSTKDYLYVLRSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGI
                     EDSLK"
     gene            1784..3697
                     /locus_tag="KRH39_002118"
     CDS             1784..3697
                     /locus_tag="KRH39_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512018.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDGISTKIVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            3690..5909
                     /locus_tag="KRH39_002119"
     CDS             3690..5909
                     /locus_tag="KRH39_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG8512019.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            6018..6848
                     /locus_tag="KRH39_002120"
     CDS             6018..6848
                     /locus_tag="KRH39_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBG8512020.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            6838..10305
                     /gene="addB"
                     /locus_tag="KRH39_002121"
     CDS             6838..10305
                     /gene="addB"
                     /locus_tag="KRH39_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBG8512021.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            10305..14132
                     /gene="addA"
                     /locus_tag="KRH39_002122"
     CDS             10305..14132
                     /gene="addA"
                     /locus_tag="KRH39_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBG8512022.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKSDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            14178..15389
                     /locus_tag="KRH39_002123"
     CDS             14178..15389
                     /locus_tag="KRH39_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBG8512023.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            15376..18903
                     /locus_tag="KRH39_002124"
     CDS             15376..18903
                     /locus_tag="KRH39_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8512024.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEIPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            19034..20287
                     /locus_tag="KRH39_002125"
     CDS             19034..20287
                     /locus_tag="KRH39_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG8512025.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
BASE COUNT         8797 a         1901 c         3620 g         5982 t
ORIGIN      
        1 tatttgagaa aaacttcttc acctattaca gaacttacag gaagtgatag atatgaaaca
       61 gcagttaaaa taagtaaaga gggttggaaa aatggttcag ataaagtagt tataataaat
      121 gggtatgtaa gtatagatgg cattatatca actccactgg caaccacata taatgctcca
      181 atacttttgg ttgaaaaaaa caatgtgcct aatagtgtaa aatcagaatt aaagcgccta
      241 aaccctaaag atataattat aattggagat gagaatgcta tttctaaaac tactgctaat
      301 caaattaaat caactgtaaa tgctagtcaa acacgtttaa atggttctaa tagatatgag
      361 acatctttat tgatagcaaa ggaaatagat aaaaatcatg atgtggaaaa aatatacata
      421 acaaatgcta atggcggaga agtggatgca cttactatag cagcaaaagc aggtcaagac
      481 aagcaaccaa ttatattaac tgataaagat agtattacag acaacacata taaatggtta
      541 aagagtgagg atttacaaaa tgcttatttt ataggtggtc ctcaaatgat atcaacaaat
      601 gttataaata aggtaaatgg aataactaag gatagtgtta ctaataatag agtatacgga
      661 gcagatagac acgaaacaaa tgcaaacgta ataaaaaaat tctatacaga tgatgagtta
      721 gaggctgttt tagtagctaa atcagatgta cttgttgatg ctttagcagc aggtccattg
      781 gctgcgaact taaaatctcc aatacttata acaccaaaga cgtatgtatc tgcataccat
      841 aaagataatt tagaagctaa atcagctaat aaagtataca aaataggagg aggattgact
      901 tctaaggtaa tgagctctat agcatcatca ttatctaaac acaatacgac tccaacagaa
      961 ccaggaaata gtgggggcaa gacagttatg attgacccag ggcatggtgg ttcagcacct
     1021 ggaaattcat ctggaggaat gattgaaaaa gattacaatt taaatacttc acttgcaaca
     1081 actgaatatt tacgttcaaa gggattcaat gtaataatga caagagacac agataagact
     1141 ttatctcttg gaaatagaac tgctctatct aattcattga aaccagattt atttacaagt
     1201 atacattata atggctcaac taataaacaa ggtcatggtg tagaagtatt ttataagctt
     1261 aaagataaaa atggagggat tactaaaact gtagctacca atatattaaa tagaatttta
     1321 gagaaattta aacttacaaa tagaggtata aaaacaagag tacttcctag tgattctaca
     1381 aaagattatt tatacgtttt aagaagtaat gatatgccag ctgtacttgt agaatgtgca
     1441 tttttggata atgaaaatga tatgagttta ataaactcat ctgcaaaagt aaaagaaatg
     1501 ggtacacaaa taggtaaagg aatagaagat tcattaaaat aaatggaaat aaaaatattt
     1561 aaaaacttaa tgtaaattta tagttgtaaa tttatactaa agtcaaaaaa tgaaatataa
     1621 gtgtatcaat agtaagactg tatgtacatt tgcacatgtg aatgcaagta tatatacagt
     1681 ctttttttgt agttaaatgc aataatttga atgtgttttg taaaatacac aatatgatat
     1741 aattagataa tatacactta atttaattga ggaaggtgat tatatggaat atttatttga
     1801 aaaaagtagt ttaaaagaat atggaaatga agctttagat aaatatagag aattattaga
     1861 taaaactggt aataattcaa ggtgtattct tttgttagta ccaaacaata acacaaagat
     1921 acgatatgaa agggctctaa gattaaatta tagtgaagaa ctgaaaataa caacatatat
     1981 aagttttgtg aagaaagaat tagttaaata ctggccacta ataatagaaa aatgtgatgg
     2041 aataagtaca aagattgtat ctccaacatt tatatcaaat agtttaagtg aatatttaat
     2101 agtgcaaaaa gttaaggaaa aaaggataca agaaggatat tttgaagata ttacaggaac
     2161 caataaatcc atagcaaata gtataatgac taacataaat aagtctgcat ttaatttaat
     2221 tgattttgat tttattggag aaaaaatata ttcatcaaag aaaaataaag atagtatata
     2281 tagattttca tacactcaaa tggatgaaat tatatcatat tatataaatt ccttactatc
     2341 aaattcaatt ttagataatg cattaagcat atatttatac aatcaatacc tacttaatga
     2401 tgatttttat ttaaaaaact tatttacaga agtgagatat ataattgtgg atagccttga
     2461 aagctcaagt aatgcagaag ttgattttat aacagaagca ttggataata cattacaaag
     2521 ttatatatat tttgattata gtagggatta ttctgtattt aataatgtgg atatagacta
     2581 tataaatgag aaaataatat caaaaattaa gcctaaagaa gaaaatatta aaaagaagga
     2641 gattagaata gaagatttat acttattacc tgcaaatata gaattaaatc aatcaagtca
     2701 attatataat gaaatgttag atttaataag tgaaaaagtt atatctctta tagaagaaga
     2761 tgtatctcca agagatatag caattatatc tcctataaac aatagtatat tggagcatca
     2821 aattagagat tctttgatag aaaaaaatat agatgttttt agtactaaga aggataaaaa
     2881 agctgtggat tatccatatg ggaatgcatt ggtagtggct acatgtatat tttatggata
     2941 tttagatttt ataaaagatg atgaatactt aagcttttta gaaactcttt tagaaataaa
     3001 taggataaaa gcatttaaga tttttaatga aacaagacat ttaagtattg ataatataga
     3061 tgaacatagt cagtatcgtg atattttaca gtatattgaa gaaaaaaaga agagtgatat
     3121 aaaaatacat gaatttttaa ctcaatttta catagataaa atgttaaatt taaaagaagg
     3181 taaaaagaat gttgggttat gtaaaaagat aatagctgaa agtgagtctt ttagtgaaag
     3241 tttaagtcta cttgggatga aaaaagaaaa gatatttgta gaagctttaa aaagtacaat
     3301 aaacgattat tactctgttg tcgatataga agagttgaaa tccaaagaca aaatagtaat
     3361 aactactcct tattcatata tatcatcaaa tatagataga agtatacaaa tatgggtaga
     3421 tataggaagt aatgcttgga atatgaaaat agaaaaagat ataagtaatt taattgtact
     3481 tagaaaatct tttgaagaaa aaaagattta tacaaatgaa atggaagaat attataaaaa
     3541 atactatcta tacaatacag tatataattt gttattaaat gcaaagaaaa tctatgcatt
     3601 taaaagtgaa tatgcgatta atggttatat ccaagagagt atattatata gtatactttt
     3661 aaaaatctca cataaaggag ataaaaatta tgattaatat aaattataga gaagaccaga
     3721 taccaataat aaattacgat aatggtacta tggcagtacc agcagtacca ggtgctggaa
     3781 aaacatttat aataacaaat ttagtagcta aactattact tgaacagaaa cataagggtg
     3841 gtaaaatatt aattcttaca tatatgaata gtgctgtaaa taattttaaa ggtagaataa
     3901 gaaagattct tgaagagaat aaaatagatg atactaattc gtatgaagta atgactatac
     3961 acagtttggc tgtaaaaata ataaaggaaa agccagaagt tgtaatgcta agtgaggatt
     4021 ttaacatagc tgacgatttg caaaaaagta ttatattaaa tgaatgtata agtagattta
     4081 gatttgaagg tggagaaaga gcttttagat ggtttttaaa ggagcaaaaa gatgaacgct
     4141 ggaaagaaat aactcttgaa gcctgggaaa gaggtttttt tgaatttgca ggaaatgcaa
     4201 taagtgaact taagtataag ggaatttctc cagataaact tgaagaaata ttgtcaagag
     4261 gacacaaagg aatgcttaaa attattttac caatatataa attgtatgat agaaaattaa
     4321 agcaaaatgg attgctagat tatgatgata tattaatttt agcagaaaaa actttaagct
     4381 tagacgaagg gcttagaaag aagtttcaaa ctagatacaa atatatattt gaagatgagt
     4441 gtcaggactc aaatgaaatt caaggtaata taataaaaat tatatctagc gaaaataaaa
     4501 acctagttag agttggagat ataaatcaga gtataacagg aacattttca tcatcagacc
     4561 ctaaattttt taaagaattt ataaatagtg cagatttctg ttataggatg gatatgtcaa
     4621 atcgaagttc aaaagatata cttgatttag caaacactct tgtcaagtat gttacaaagg
     4681 aatttagaca gaaagaatgt agacatgctc ttgaagatat gcaaataaaa actgtgccaa
     4741 atggaatggg atataaagaa aaccctaatc cagaacatta taatataaat gtaaagtggt
     4801 atgagagttg gaaaattgaa atagagcaaa ctgctagata cgttaagggt ataaaaaata
     4861 aatatccaga taaaagtata ggtatattgg ttccatttaa tgaacaagtg acacaagttg
     4921 ctaaagaact tagagagaat aatctaacct ttgaagaatt aggaccaaat tctttgaata
     4981 aaaggaaggt tataaacaac atagcatata ttattgattt tatgttgcat tgtgatgatg
     5041 tagaaaagtt aatagttgta cttaataaag tttttataga tactgataat gaagaagaaa
     5101 taaagtattt tttagaagaa ttaaaaaact attctaccga agaaattatt tataattttg
     5161 aatttagtaa gttagataat gatttaatag aatttaaaga agaaagtgat atatacttaa
     5221 gttttaaaag aggaatagat attataaaac agatattaga atatccaata ataaggttag
     5281 acctcttagt tttgtttatt gggaaaaaac ttaacttaga aaaagaagat aaagctgtac
     5341 tggattatat atctttttat ataaagtatc tagtatcaga aaatataaat atggatttag
     5401 aagatgtata taatttattg tttgatgtaa agaacagggt gtttaatcat ataatagatg
     5461 tggtatatga gataaatgga tatgaaccag agcctggtag tataacgcta tgtaactatc
     5521 ataaatcaaa ggggatggaa tgggattgtg tgtttttact aggtcttgtt gaatataatt
     5581 tccctgacaa tattaatcaa aagtttcaga gtgataaatg gtatctaaaa gaaaaatata
     5641 aaaaccctat ggcaattatt aaatcagaag ttgaagctat attaaaagga agtatttcaa
     5701 cagattatgc tcataaaacg aagattgatt ctataaatga aaagattaga cttttatatg
     5761 tagggattac tagagcaaag gaaatgttag tactttcagg aagtgcttat agagatgaaa
     5821 gtgatatagg gaataagaga aaagagcaaa aaccatgtat atatttaagc agacttaatc
     5881 aacatattat tgaaaaaagg tcaaattaat atataataag ttattaaaag attgcttgaa
     5941 atatatctaa aaggggataa aacaatatgt acagtaagta actactaaaa aataaagtta
     6001 agagagaagt gatatgagtg aacgataaat taaaatattt ttcttatagt caaaattcaa
     6061 ttaacacata taaatcatgt ccattgaaat ttaaatataa atatattgat agaataaatt
     6121 ggaaaaatga tgatgttggt agtagagagt attatgaaac tttaaaaaca ggaagagatt
     6181 ttcatttgat ttgtgaaaga tattttagca atatacctct tggaatctat tttaatgagg
     6241 atgataaaaa tagtaaaaag tttttaaagt ggatggaaaa tataaaaaaa gttgttccta
     6301 ttgaaaaagg aaaaacgtat ttgccagaat atgaagtaag gatgacatta aatggggata
     6361 taatacaggc aaagtatgat ttagttgtaa ttggaaataa caatatagaa atatgggatt
     6421 ggaaaaccga gtctaaaaag attgactata aacacgtaga aaatagaata caaactattg
     6481 tttatatgtt tttagcaaaa gaagtaatac ctaaagtttt aaaaatggac ataaatgtta
     6541 aagatataaa tatgagatat tatcaacctg aatttgatga cttaccaatt accattttat
     6601 atgatgaaga aaaacatgaa gcaaatagaa ataaaattca aaattatata agtatgataa
     6661 aaaacactaa ttatgaagaa catacatacg aagacaatct atacaatgat atagaaaagg
     6721 tatataataa agaagaacgt atgtgctata gaaataaaaa gcattgcaag tattgtgaat
     6781 ttaataagtt atgtaatggc aaagaaatag attatagtat tttggaggcg gaaatttatg
     6841 ggacttaggt ttgtattagg tagaagtgga agtggaaaat ccacctatat actagatgag
     6901 attaaaaaag aagctcaaaa aaatgaaacg acatcaatta tacttttagt tccagaacag
     6961 tacacatttg aggctgaaaa tagagtaagt aagctattct taggaaaaga aaaagataag
     7021 tatctaagag ttagggtact tagctttaag acactaagta atatagtttt ttctcaagta
     7081 ggaggattga cagatgtaaa tataaattct agtggaaaag caatgatggt atatagagct
     7141 attgaagatg taagtgaaga attaaatgtg ttttcaaaat caaaatcgca gtctggtttt
     7201 gtaagttcaa ttacagatat gatatcagaa atgaaacaat ataatataag tcctgaaatg
     7261 ttagaaaata tatctggaga attggataat gaaactttaa gtttaaaatt aaaagatata
     7321 agtaaaatat ataattcttt tgaagggaaa ttgcatgaaa attatgtaga tgctcaagat
     7381 atgttaactt cattggcaag taaaattgaa ttaagcagtt atttagatgg agcttgtgtt
     7441 tatatagatg aatttacagg ttttacacca aatcagtaca atgtcataaa gagtatatta
     7501 aataagtcta aaagtgttaa tatctctttg actgtagatg atatcaacta tattgggtac
     7561 agtaaatctg atatgttttc tagaacgaaa tttacttatt caaaattgac tcaattgtgc
     7621 aacgaagaag gcataaaaat attgccacaa gtaaatttaa acacaggtgt tataaaaaga
     7681 tttgaaaaag ttaaggaatt acagcactta gaaagatttt ataatgctta tccatataaa
     7741 atatattcaa atccaactga aaatataaag ataaaagagt ttaacaattt atattcagag
     7801 gtggaagaga tagccagaga aattgtacat ttagttagag ataagaatgt aagatataga
     7861 gatataacca ttgctacaag agatttaaat agatatgatt ttttagtaca ctctatattt
     7921 aatgaatata atataccaaa ctttatagat aagaagagag aggcaaaaag taatccaata
     7981 gttattttaa ttatttcagc tttagaaatg aagaatagac gatatgggta tgaaactatg
     8041 tttaggtatt taaaaagtgg acttatagga attgataatg atgatataaa tttactagaa
     8101 aattatgtgt tggctaatgg gattaaaggt aaaaaatggt ttgatgaaaa atgggattat
     8161 agaataaccc aaagtctttc aggtcaagaa agtgaatttg aactggaatt aaaagagaaa
     8221 ataaatgaaa taaaaaatag agtattagag cctatagtaa ttttacaaga aaaattaaga
     8281 ggaaaaaata gagtaaaaga aatctgtaga tatatatatg aatttttatt agatataaat
     8341 atgccagaaa ctatagaaag tttaatagta aattttaagg ataaagggga acttgatgtt
     8401 gcaaatcaat actcacaagt ttgggacata gtagtggaca tattagacca aatggtggaa
     8461 cttatgggtg atgaaataat ttctttagaa aaatttataa aattaataac cttaggtttt
     8521 gatgaatatg aattaggact ggttcctcct agtatagacc aggtacttgt aagtagtgtg
     8581 gatagaatga aaaatccaga cacgaaatat ttatacctag ttggaactac tgatggtgtg
     8641 tttccgctga ttacaaagga tagtggaata ttgagtgata atgataggga aagtcttgga
     8701 aacaaaggaa tcgaagttga tattgatagt aagactagga gttttgaaga acagtttctt
     8761 gtatataaag ctcttacttc gacatctaag aatttaacca taacatatcc aatatcagac
     8821 catgaaggta aaacgcttag accatcaata attatatcaa gacttaagaa gatatttcca
     8881 aatatagaaa ataaaagtta tttagtggaa gaaaataaaa gcactgataa agatatactt
     8941 aaaaaaataa cggttaaatc ccctacattt aatgaactta taaacgtaat aaaaaattat
     9001 gacagtgatg gttacaatac agaagagatt aatagtattt ggttagatat atataggtac
     9061 tatttaaaag atgaaatcta tagttctata acaaaaaaag ttataaaagg tcttagttat
     9121 actaatcaag ttcataagat tgaagagaaa aaaataaggt cattgtataa aagtaattct
     9181 cttagcatat ctaggcttga aaaatatgct gaatgtccat ttgcttattt tatacaatat
     9241 ggtctaaaag caaaaaagcg taaagagtat tcatttacac caccagattt aggaactttt
     9301 attcataata ttttagatag gttttcaaaa gaacttttac aagataattt aacttggaga
     9361 gatattgatg aaaaatatat agaattaaag ataggtataa tagttgatga aataatttta
     9421 aagataccag gatatatatt aaatagttct gagaggtata aataccttgc atatagatta
     9481 aagaatatgt tgacaactgc tataactata attagtcagc aaataaaaca aggttcattt
     9541 gaaccaatag attatgaagt taaatttggc gacaatggaa aatatccacc tataaaaatg
     9601 gtgttagaaa atggacaaga agtaagttta attggacaaa tagatagagt tgatgaattt
     9661 gaagaaggag aaaataaata tataagaata atagattata agtcaggcaa taagtccata
     9721 agtctaacag aaatatatta tggtctacaa ttacaattac ttgtgtattt agatgcaatc
     9781 cttgaaagtg caaaggatga agatataaat ataaatccag ctgcaatact atattgtaga
     9841 ataaataatc caatagcaaa atttaatgaa gataaagatg atgaagaaat acaagaagca
     9901 atattaaaag aacttagaat gaaggggctt gtagtaaaag actctcacat agttaaagag
     9961 atggataaat cattaataga tggagaaagg aaaaactctt tagtaatacc agtaggtctt
    10021 actaaggatg gaaatgtagg taaaagtact tcagcaataa gctatgagga tttcaaactg
    10081 cttagaaaat atgtaaggca tgctataaag gatttatgtg aagaaatgtt aagtggagaa
    10141 atacgtatag ctccatataa acataaagat ggaacatcat gtgacttttg tgattactct
    10201 gctatatgtc agtttgattc aactataaaa gataacaagt ataaaaattt aaataataag
    10261 agtaatgaag agataataaa aatgatgaaa ggagatgtta attagtgagt tctcctaaat
    10321 ggacaaaaga gcaattagaa gttatagaaa gtagagaatg caatttattg gttgctgctg
    10381 ctgctggttc aggaaaaaca gccgtgctag ttgagcgtat aattcaaatg ataacaagta
    10441 gagaaaatcc aattgatata gataagttat tagttgttac atttactaat gctgctgcgt
    10501 cagaaatgag agagaggata ggggatgcta taggaaaagc cttagatgaa aacccagaaa
    10561 ataaacattt acaaaaccaa cttgttttat taaataaatc tagtattaca acaatacact
    10621 cattctgttt ggatgttata aaatcaaact ttcatagaat aaacttagac cctaatttca
    10681 gaataggaga ccaaactgaa tgtgctattt tgaaacaaga agcaatagag gaagtatttg
    10741 aagatttata tgaagaaaga gatgaggggt ttttaaattt agttgaaagt tatgcagaaa
    10801 gaggaggaga taaagaagta caagatatta tcttgggaat atattcattt gcaatggctt
    10861 ctcctgaacc taaaaaatgg ttgattgact cagcagaacg atttaatatt gatgaaaatt
    10921 ttgatttttc acagtctatt tgggctagag ctatattaga tactgtaaaa atagagatta
    10981 atggtctttg tctaaatatg gaaagggcat taaaagaagt ggaatcaata gaagaattag
    11041 aaacttttgc agaaaagtta tccgttgaat ataaaaaaat tgctgacatt tcacaagcat
    11101 gtaataaatc ttgggatgag gcatataaaa agatggcaag tatgtccttt gaaaattatg
    11161 taaaaggagt aaaaagaatt tcaaaagatg ctccttcgta tataaaagaa tcaaaagaaa
    11221 aggcaaaaac tataagagat aagactaaga agtctttaga aagtatagta agtgctacat
    11281 tcaataaaga taatgattct attagagaag aaataaaata tctttataac atagttaaac
    11341 caatatctag tgttgtactg agatttgaag aggaatatag caataagaaa agagaaaaag
    11401 gaataataga ttttaacgat atagagcatt ttgcattaaa tatacttaca gatgtagatg
    11461 aaaaaggtaa tattgtacct tctgatatag cagttggata tagaaacaag ttttatgaaa
    11521 tatttataga tgaatatcag gatagtaatc tagtgcagga agtactgctt aaagctgttg
    11581 caaatacaga aactccaaat agatttatgg ttggtgatgt aaaacagagt atatatagat
    11641 ttagacaagc aaagccagaa ttatttttgc aaaaatataa taactataat gataaaaagg
    11701 gaagctccca tagaaaaata atgctttaca aaaactttag aagtagagaa gaagttgtag
    11761 atgctgtaaa ttatatattt gaaaatataa tgaatgagaa tataggagaa attgaatata
    11821 cagaaaaaga aagactaaat ttaggtgcaa attttaatgt ggatacagac gagaaatcta
    11881 ttattggtgg agcaacggag atacacctaa tacaaaaaga taataaactt gatgatgata
    11941 ttataaatga taaagacgat agaattaata acaaagaaaa tgaaatagag gaagaagaaa
    12001 aattagataa tatacaactt gaggctagaa tggttggaaa cattatcaaa gatttaatga
    12061 aagtcaatga agatggaaaa attcaaaagg tctatgataa gggaatagat ggatatagac
    12121 ctgtggaatt tagagatata gttattcttt taagagctac atctgcatgg gctccagtgt
    12181 ttgcagatga attgatgaat atggatatac caacgtatgc tgatgttgga gtggggtatt
    12241 ttgatacgat tgaaataaaa acaatattgt cattacttca aataatagat aatccaatgc
    12301 aagatatacc tcttatatct gtattaaaat cacctatatt tggatttaca ccagaggatt
    12361 tgattgatat tagagttcag agtaaagata aaatttttta tgaggtttta aaaagtacag
    12421 cagagtatga tggatttaca gattctcaaa atgaaaatga gtctgagttt ataccaagtg
    12481 aagaatgcat aaacaaaagt aaagattttt taattaagct aaaggaattt aaagaaaagt
    12541 caatgtacat gagtacagat gaatttatat ggtacttgta cacaaggact ggatactatg
    12601 cctatgttgg agcattgcca ggtggaagtc aaagacaagc taatttaaaa gtattgtttg
    12661 agagagcaaa acaatttgaa gaaaccagtc ttaaaggaat attcaacttt gtaaatttta
    12721 tagagaaatt aaaaaaatct agttctgata tgggaagtgc aaaaacactt ggtgagaatg
    12781 caaatgttgt tagaataatg agtattcata aaagtaaagg tctagaattt ccagtagtca
    12841 tatgctctgc aatgggtaaa aatttcaata ctcaagattt taaaaagagt attttatatc
    12901 atcataattt agggtatgga cctcaatttg tagactatga gagaaggatt tcttttccaa
    12961 gtatagccaa agaagcatta aaaagcaaaa taaatataga aaatttatca gaagagatga
    13021 gagttttata tgttgcattt acaagagcta aagaaaaatt gattataact ggttctacta
    13081 gaaatataca agatagtata aagagatggt caaatggaat tgaaagttta gatacaatat
    13141 cacaatacga gatattaaaa ggtaaaaatt ttttagattg gataatgcct tgtgtattaa
    13201 gacataggga tttatctaat ctactagaag aagtaggatt agacgcagtt tttaatgttg
    13261 aacataattc aaaatggtat ggaaagttgt ggaataagag tgatatttta gtggaaaaga
    13321 agagtgatga agaaaaagaa agtattgaag aaattcttga aaaaatagat gtagataatc
    13381 ctgatagtga ttattacagt gaaatagaag aaaaattaaa ctatatatat ccatatgaat
    13441 ttagtacaag aaaacctgct actatatctg ttacagaaat aaaaaagata caaaataatt
    13501 atgaagaaga gttaataaat actatatttg aacaaaaagt aatattaaaa aagcctctat
    13561 ttattcaaaa tgaagaagaa agagaaaaaa taagtggaac agaaagaggt acaatagtcc
    13621 atttagttat ggaggtttta gacttaaaaa atgtaagtag tgtaaatgat ataaaatcac
    13681 aaataagagg ttttgtttca aaaggtataa taacagaaaa acaagctagt atagtaaatc
    13741 catataaaat atataaattt tttgcctcta acataggaaa aagaatgttg aatgctgaaa
    13801 ttataaatag agaaaaatca atttatgcac aagtaaatat gaaagacata tatatttatg
    13861 aaaagcttat aaacaatgac gataaaaaac tttatgataa tgaaagtgtc atgttaagag
    13921 gtatagttga tgcttatttt gaagaagaca atcaaatagt tttggtggat tataaaacag
    13981 attttgtaaa tgaggaaaat ataaatcaaa taatagagaa atataaaaaa cagttggatt
    14041 tatatgcaga tattattgaa actttaactg gtaagtcagt aaaagagaaa tgtatatatt
    14101 tatttggagt tgatgaagct gtttgttatt agtttttata tataaattaa acctaaaaga
    14161 tagaggaagg ggaaaaaatg agatttattc atacatcaga ttggcatcta gggaagtccc
    14221 tagaaggcca ttctagaatt gaggaacaag caaagttttg tgaggaattt ataaaaatag
    14281 tagaatcaaa tgaaatagat atggttataa ttgctggaga tgtatatgat acttctaatc
    14341 caccagcaca agcagaaaaa ctattttatc agactgtatc aagacttgcc aataatggtc
    14401 agaggtgtgt attaataatt tcaggaaatc atgataatcc agagagactc tctgcaataa
    14461 cacctctagc acatgaacag gggatactta tatatggtta tccattaagt gctacgatag
    14521 aagctaagta taaagggttt gaaataacat atgcaactca gggatgtaca aaactgaata
    14581 taaatggaga aaatattgtg gttgctacat taccttatcc aagtgaaaaa agattaaatg
    14641 aagttttttc aagtgaagat gagtttgaga aacaaaaaaa ttattctgaa aaggtaggag
    14701 atatatttag aagtttagaa gaaaatttta gaagtgatac tataaatata gccgtatcac
    14761 atatatttgt aataggtgga gagagtactg aatcagaaag acctatacaa ttaggtggaa
    14821 gttttttagt tgaaagaaaa gatttgccag aaaaggctca atatactgca ctaggtcatt
    14881 tacataaaca acaaaaagct tccgaacgtt taaatgcata ctattctggt tctccacttc
    14941 aatatagcaa ggatgaaaga gcgtatacca aaggggcata catagtggat ataaaagctg
    15001 gtgaaaaacc aataattgaa gatgtatatt ttaataatta caaacctata gaagtattta
    15061 aatgtaatgg aatagaagaa gcactagata tatgtgagca aaatcaagat agagagattt
    15121 ggtcatattt tgaaataaat actgatgaga taatatcaca gaatgaaatt aaaaaaatga
    15181 aggaactatt aaaagacata atagaaataa aaccaataat aacatcttgt tatgaacaag
    15241 aaagtgtaga tataaaagaa aagtctatgg cagagttgtt tagagagttc tattctttta
    15301 gtaaaggtgt agagccaaaa ggagaactta tggatttatt tctagatata ataagtgaag
    15361 aaggtgaatc agcagatgag accaattaga ttagaactta cagggcttaa tagctatatt
    15421 gataaacaag ttatagactt tgaaaaatta attgaaagag gtttgtttgg gatatttgga
    15481 actactggaa gtggaaaatc tacaatctta gatgcaataa ctattgctat gtatggaaat
    15541 atatccagaa atacaaaaga atatataaac agtgtgtgcg ataaggcaat tatatcatac
    15601 gagtttgaga taggaagcaa aaacactaga agaagataca ttgtagatag aaccattgct
    15661 agaagcaaaa caggaacaaa gacctctaat gctagattaa tagaagtatt gaatgacaat
    15721 acccaaaatg ttttagctga taaagttgtg gaagtaaatg aaaaagtagc gcaggtcgta
    15781 ggattaacgg caaatgattt tactaggtca gtagttttac cacaagacaa atttaatgag
    15841 tttctaagat tatcacgtgc agatagaaga gacatgttag agagaatttt taatcttgag
    15901 aaatatggaa gaagtcttgg cgaaaaagtg aaaaagagaa aaaatataca gttacaaaat
    15961 ttaaaagatt tgaaatcaaa attaagtcaa tatgatggaa ttacagagga agtatataac
    16021 aatataaacc aagaattaat agagttaaaa aatttggaaa aagataagaa taatgcttta
    16081 gacctagctc aaaaatctta tgaagaaagt aaaacagtat atgaagaaca attaaagtta
    16141 gaaaaaaatg aacttagaaa aaatgagctt gatttaaaaa atagtgagat aaaagaaaag
    16201 attgctcttg tagaaaatga tagtaacgct aaaaaggtag acccatacat aagttcagtt
    16261 caaaatctag aaaaaaagat agatgaagat agcttcgcag taagtaattt agaaaagaaa
    16321 ttagcaatat taaatcagga gcttgaagtt acaaaaaata gatatgaaaa aataaacaaa
    16381 attaagaatg aagaaatccc taaattgagt gaagaaaaaa taagattgca acaagcaata
    16441 aaattagaag aagagttagt tttattagat agagagttaa aagagctaaa agaaagtgga
    16501 attaatttaa ataagactaa agttgaatta gaaaaagtaa agcaggtgtc agagtccaga
    16561 aaagatgcag ttactaaatc tataaaagaa gttgaaggaa agattgataa agtaaatata
    16621 agtgcagaat taaaacaaaa aatatttcta gcatatgaat acgagaaaga ttacaacaac
    16681 gtattggaag aaaaaaatca aaagctaaat aaactagaag aaatactcaa agagacagaa
    16741 aatataaatt taaaagttag atatattgat aaagataaga atgatgtaaa tcgtaattta
    16801 gaaaatttat cacttcactt ggatgttctt cttaaaaaat gtcctggtaa gagtgctgat
    16861 ttacttttaa aatctgaata tgttactgaa ttaagaaata aagcaaataa tactaaagaa
    16921 aatgaaatta agaaaagcag tatacaagat gaattaaaga taatacttga aagtaagttt
    16981 aatactgaaa gggaagtaaa tttactaaat gaaaaacttg aaaataatag aaagaacaga
    17041 gatgacttag aaaaagaatt agaagaacta aagtatttaa atttagcctc tgaacttaga
    17101 cgtgaattaa aagaaaatat gccttgtcca gtttgtgggt caaaacatca tgaaaatcat
    17161 gatataacta agtatgatga aaatatatct tttgtcaaag aaaaactgga gaaattagaa
    17221 aaagaaaaaa taagtataag aaacaatata gaggaattaa atgctaaagt aagtgggtat
    17281 ttatctatag aaaaaatgaa gactaaagaa cttgaagatg taaaaggaaa actagggggg
    17341 attccatcta gtcaattatt aaaaaaactt gatgaagaac aaagaaaact tgcattgtta
    17401 aaatctaata ttcaggagtg ggagaaagaa aaagagagta ctgaaaataa agtaacatta
    17461 gctaaagaag aaaaaaataa aattgaaaaa gaagaattaa aaataagaga gagtctgaat
    17521 aattataaga aattaacaag agatttaaac atagaaatag aaggtttaga aaataaatgt
    17581 aaaaaactta aacaggaata cctaggatta aagacgatta ctaaagtctc taatctattg
    17641 tcaaaagtaa aagaaataag ggagaatgaa aaagaattag aattactaag tactgggcat
    17701 tcaaatttat taaaaaatag agactcacta gatattaaaa taagagaaaa tgagagtaaa
    17761 ctacatgaaa tagagataga attaataaag gctagagaat tatatgtaga aaagaaattg
    17821 agtagagata ataagtataa agaagttatt agcataacga aaggtgattt agctaaaaat
    17881 cttttacata atgttgaaga aaatatatgt aagatattag aacaagaaga atcaagtaag
    17941 aaaaaattgg aagagcaaag attagaatat gaaaaaaaca ttgctgaaaa gcacaatata
    18001 gatggtagac ttaaaactgc taaggaacaa tataaagacc aaaaatcaac actaaataaa
    18061 ctattggcag aaaataaatt tgaaagtata tatgcagtaa aaagagcttt attagatact
    18121 gatgcagtaa aaaaattgat tgaagaaata ctagagcatg aagaagaaca aaagttatta
    18181 tcatttaaaa taaaatcatc aaaagaaaaa ttaaatggaa gaagcataaa gaaggagtac
    18241 tttgaacagt taaaagatga aatttataat ttgaaagtag aaataggaaa tatatccaaa
    18301 gatattggag ctaatcaaaa tcagttaata acattaaaag actctttaga taagataaat
    18361 gattttaata aacaacttaa ggtagttgaa cacaatgtgg acttattgga agaattagat
    18421 aaatgtgtac aaggaaataa atttgtagaa tatgtttcta caaaccaatt aaaatatata
    18481 gcgttggaag catcaaaacg attggatgga ataacaaaag gaagatatgc acttgaaatt
    18541 gactcaacac taaactttgt aatgagagat aactttaatg gaggagaaag aagaagcatt
    18601 gatactctat ctggtggaga gacattcttg acatctctat cattagcact tgctctttca
    18661 tctcaaatac aattaaaggg aagtgcacca ttagaatttt tctttttaga tgaaggtttt
    18721 ggttcattag atagtgaact tttagaaatt gttatagaat ccttggaaag attgcatagt
    18781 aataatctaa gtgtgggtat aataagtcat gtcgaggaac ttaaaaatag agttcctgtt
    18841 aagctattag tttcatctag tgaggctgga ataggttcaa aagtaaaaat tgaatatagt
    18901 tagaaaatat atgttaattg aatatattat ctaatatttc caaataatat gtaattgtga
    18961 tataataaaa actaatatta aagttattta taattaatat taatataaaa acatgtaagt
    19021 aggagtgtgg taagtgttgg aatcgcccaa taatttgatt cagattattt ttttaatagt
    19081 attacttata gggtctgcat ttttctcagc atctgaaacg gccttaatgt cgttgagtaa
    19141 aatcagaatc agatatatgc aagatgaagg agttaaagga gctaagttag taagttcatt
    19201 aatagaaaat ccaaataagc ttttaagttc tatattggtt ggaaataacg tcgtaaatat
    19261 agcagcaaca tccatatcaa catctttgtt tataggatta atgggagaaa aaggagtggc
    19321 acttgctaca gctgttatga cagtattagt attaatattt ggtgagatta ctccaaaaac
    19381 aatagcagca aataattcag aaaaggtttc tctattagta tctaaaccta taaaagcaat
    19441 tatatttata ttaagaccaa tagtatggat atttaatata attacaaata ttatatttaa
    19501 attatttggt ataacaaata aaggagctaa atcattcata actgaagaag agctaaaaac
    19561 tatggtaaat gtaagtcatg aagaaggcgt ccttgaaatg gaagagagag aaatcataaa
    19621 taatgttttt gaatttggag atatgcaagc taaaaatgca atggtacaaa gaatagatat
    19681 ggtagctata gatatggaag atagttatga tgagataata caggtattta aaactgaaaa
    19741 attaagtaga atgcctgtgt atgaagaaac tatagatgat attgtaggaa tacttaatat
    19801 aaaagatatt atatttttat ctgatgaaga aatagagtca tttgatatta aaaattatat
    19861 gagagaacct tttttcacgt atgagttcaa aaagataaca caacttcttg aagagatgaa
    19921 acttgaaaaa agtcagatgg ctatagttgt agatgagtat ggtggaacat ctggactttt
    19981 aactatagaa gatttagtag aagtcattgt tggagatata gaggatgaat atgatgaaga
    20041 agaggatgaa atacaagtta taaaagaaga tgagtatatt gtagatggaa gtactaaaat
    20101 tggtgatgtt aatgaactta ttggtgtaaa cttggaatct gaggagtttg attccatagg
    20161 aggttttata attggacacc taagcagatt gccagaagaa aatgaagtaa ttgaagttga
    20221 taatataaga ttttgtatag aaagtataga aaaaaataga attaaaaaaa taagaatata
    20281 tacataatgt aataatgttc
//
LOCUS       DAEORZ010000066        20080 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEORZ010000066 DAEORZ010000000
VERSION     DAEORZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.066"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(134..937)
                     /gene="proC"
                     /locus_tag="KRH39_002126"
     CDS             complement(134..937)
                     /gene="proC"
                     /locus_tag="KRH39_002126"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG8512026.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            complement(1262..3631)
                     /locus_tag="KRH39_002127"
     CDS             complement(1262..3631)
                     /locus_tag="KRH39_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBG8512027.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRSFKSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            complement(3636..4544)
                     /locus_tag="KRH39_002128"
     CDS             complement(3636..4544)
                     /locus_tag="KRH39_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG8512028.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            complement(5286..6029)
                     /locus_tag="KRH39_002129"
     CDS             complement(5286..6029)
                     /locus_tag="KRH39_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG8512029.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHKWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNSNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYKNI"
     gene            6195..8762
                     /locus_tag="KRH39_002130"
     CDS             6195..8762
                     /locus_tag="KRH39_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8512030.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERINIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            8992..9396
                     /locus_tag="KRH39_002131"
     CDS             8992..9396
                     /locus_tag="KRH39_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8512031.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            9420..9917
                     /locus_tag="KRH39_002132"
     CDS             9420..9917
                     /locus_tag="KRH39_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8512032.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNHNINRY
                     KGEFI"
     gene            9920..10321
                     /locus_tag="KRH39_002133"
     CDS             9920..10321
                     /locus_tag="KRH39_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBG8512033.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            10339..10827
                     /locus_tag="KRH39_002134"
     CDS             10339..10827
                     /locus_tag="KRH39_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8512034.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            10854..11609
                     /locus_tag="KRH39_002135"
     CDS             10854..11609
                     /locus_tag="KRH39_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8512035.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            11609..12472
                     /locus_tag="KRH39_002136"
     CDS             11609..12472
                     /locus_tag="KRH39_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG8512036.1"
                     /translation="MQGIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            12519..12998
                     /locus_tag="KRH39_002137"
     CDS             12519..12998
                     /locus_tag="KRH39_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBG8512037.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGVNPDNIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLEFAQKCAHDDEVCELVFTRK"
     gene            13063..14370
                     /locus_tag="KRH39_002138"
     CDS             13063..14370
                     /locus_tag="KRH39_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG8512038.1"
                     /translation="MDINRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEVGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMECIKYYLY
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPYHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIGVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            14680..14820
                     /locus_tag="KRH39_002139"
     CDS             14680..14820
                     /locus_tag="KRH39_002139"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBG8512039.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDIYPDSGWGFMTWDEN
                     GE"
     gene            15330..16424
                     /locus_tag="KRH39_002140"
     CDS             15330..16424
                     /locus_tag="KRH39_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8512040.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            16429..16629
                     /locus_tag="KRH39_002141"
     CDS             16429..16629
                     /locus_tag="KRH39_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBG8512041.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            16629..17546
                     /locus_tag="KRH39_002142"
     CDS             16629..17546
                     /locus_tag="KRH39_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512042.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLRNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            17543..18301
                     /locus_tag="KRH39_002143"
     CDS             17543..18301
                     /locus_tag="KRH39_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512043.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLIIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTIYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            18549..19622
                     /locus_tag="KRH39_002144"
     CDS             18549..19622
                     /locus_tag="KRH39_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG8512044.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDKAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGSVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            19692..19955
                     /locus_tag="KRH39_002145"
     CDS             19692..19955
                     /locus_tag="KRH39_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512045.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
BASE COUNT         7320 a         2509 c         3334 g         6917 t
ORIGIN      
        1 tcactcaatc attaaattcc tttttatagt aaattactct tattttactt ataccaaata
       61 catttatgta tagatataaa taagcactaa aaaaacaatt caagaaacca tatagtaaac
      121 ttaattacat ctgttatttg ctcatatctt tagacttatc tatacaagca acaatagatt
      181 ttataactgc tgctctaaag ccttcttctt ctaatacctt aacagcttct atagtagttc
      241 ctgctggaga acaaaccata tcttttaatt cacctggatg ttttccagtt tcaagaacca
      301 tcttagctga ccccattact gcttgagatg caaatttata agcttgttgt cttggcattc
      361 cagctaatac tgctgcatct gccatagctt ctataaacat aaatacatat gcaggagaag
      421 aaccactaat acctgtaact gtatccatta aatattctga tactatttct gcctcaccaa
      481 aagattcaaa cagagcctta atcatattta actcttcatc tgttacatta ctatttttac
      541 aaagagctga cattccttcg ccaacaaggg ctggagtatt aggcattact cttactattt
      601 ttttatctga acctataaaa ctttccagac tttctatagt tttacctgct gctatagata
      661 ctattatttt atttgcatct attaattctt taagtggagt taaagccttt tcgcatatat
      721 taggtttaag tgctactatt accacatcag aatttgtaac agctaccttt gaatcagttg
      781 tagtatttac tccaaattct tcttttactt tatctaaggc aacttggctt aaatctgata
      841 cagttatatc ttctgccttt actaatcctg actgtactat gccacctatc attgcacttc
      901 ccatattccc tgaaccaata aatcctaaag ttttcatatc aaatctcctt ttttctatta
      961 gttttatttt aaagtagcta aattgacttt attttaactg cttaaaatta tcttattttt
     1021 ataaaatata accttattat aactataata aattattctt ttgtatgtat tacttactaa
     1081 tatgtgtatg atttactaaa tataaattta acgatttttt cattaaactt aacgagctaa
     1141 cttcctattt agaaagttag ctcataatta atctaaaatt ttaaaaatgg aatcaaacca
     1201 tttatacttt attatatcag ttacgatttt atatagtaag aagttttatt tacaagcaaa
     1261 cctagaatgt ttgctcagtt cttcctatta tctcatcttg tagtgttcta cttaaattat
     1321 tgaaatggtc actatatcct gcaactctaa ctattaagtc tttataatcc tgaggattct
     1381 tttgagcctc taacagtaca tttttatcaa atacattaaa ttgtatatga tgaccatcca
     1441 tattaaagta tgctcttact agattagcca tattatctaa ccctttttct ccttgaacta
     1501 ctgatggtgc aaatctttga tttaataatg ttccaccagt ttttatgtgg tccatcttag
     1561 cacaagattt tataactgct gtaggtccat tagtatctcc acctttttca ggtgaaattc
     1621 cctctgaaac tggttttgca caaagtctac catttggact tgctcccata atttctccaa
     1681 agtatatatg acaagttgtt ggtaacatat ctactctgta ttctccacca caaacagttg
     1741 gtctaccagt tacctcattg taatatgcat tgaatatctc ttgcattatt tcatcagcat
     1801 aatcatcatc atttccatat ttaggagtct tatttgaaac taaattaagt atagcttcat
     1861 atccttcaaa attagcatca agtgctttaa gcaatgtatc catatcgaat tttttcttat
     1921 caaatacatt atattttatt gctgttaaac tatctgttat agttcctata ccaacacctt
     1981 gtatatatct agtgttgtat cttgctccac ctctttggta atccttgcct ttttctatac
     2041 aatcatcaac tattgtagac ataagtggac aaggcatgtg ttttgcacat atattttcta
     2101 tcacagcatt tccttctatc tttatgtcaa tcatataatg taattgcttt ttaaatgctt
     2161 caaatagttc ctcataagat ttaaaacttc tagggtcccc agtttcaata cctatttgtt
     2221 ttttactaat tgggtcatat ccattattta atgttaattc aagtattttt ggaatgttca
     2281 tatatccagt taaaacatat gcttctttac caaaacatcc agtttcaaca caaccactag
     2341 ttccaccaag tcttgcatct tctatagttt ttccagcttc cataagctct tgaactattg
     2401 cttcagtatt ataaaatgct ggttgacccc atccttttct tgatatttca caagctctct
     2461 ttaagaattt ttgaggagtc tttttactta tttgaacatt tgaactaggt tgtatcaatt
     2521 tcatttcatc cataacatca agtattatat aactaacttc attaacacca tcttgaccat
     2581 ctggatttat tccaccagtg tttatatttg caaaatctgt atatgtgcta ctttctttta
     2641 atgttatacc aacttttggt ggtgctggct gattgttaaa tttaacccat aaacattcaa
     2701 gtaattcttg tgctctatct ctatctagta ttccattttc tacatctctt tcatagaaag
     2761 gatttagatg ttgatcaagt cttcctggag taaaagcatc ccatatatta agttcagtag
     2821 taacccctat atgaacaaac caatacattt gtatagcttg atgataagtt tctggtttgt
     2881 gagctggtac aacatcacaa gtttcagcaa ttagtaataa atcttttttc ttagcttcat
     2941 ctgtttcttc ttctgccatt tgtcttgctt tctcagcata tcttttacct aatataacca
     3001 ttgcatcaca agctattgcc atagcttcta agtcagcttt tttattatat gcatctaaat
     3061 cattaagaaa atctagctct tttaatcttg cttctatgtc tttctttaaa tctaaaaatc
     3121 cttttttata tattgtatct ccacaaactg tatgtcctgg ggctctttgt tccataaact
     3181 ctgtaaacat acctgcttca tatgctgcta accactcagg tgtcattgca tttattatct
     3241 tatctcttgt ttgtctcttt ttccaaaaag gaattatttc ttctttatgt atttttctag
     3301 cttcttcaga tacgctaaaa tttattatat ctctattgtg cattacttct aaatcttcca
     3361 ttgtatgaca acaaatttct ggatatgttg gtgctccatt aggagaatca cctttttcac
     3421 ctactattaa ttctccatca tttatgctaa gagttctatt ctcaatataa tgcttaaatg
     3481 ataaagcacg aagtacagga atctctacac ttccctcata ttttttataa gcttctgtca
     3541 taagtactgc tctttcaata gatatgtgtg gttctgcatt aatactttct tctcttaatt
     3601 ttttagttct ctcaaaagtt cctcttgcca ttttattacc ctccaatctt agtattgaaa
     3661 ttacttgctt caaacagtct ttttatttcc tctaattttt cttctgatgg tctttgtagt
     3721 tcttctcctt catacttttt ataaatttta tcatacttgt gtttaccaat attatgatat
     3781 ggaagaagac ttactgcttg tatattcaaa ggttttaaaa actctatcat ttttttaacc
     3841 tctaaatttt catcatctac atttactcct acaattaatg gtattcttat atttatgtta
     3901 gcacctaatt cacttaaaat cttaacattt ttcagtatta agtcatttga ttttcctgta
     3961 aattttatgt gcttatcttc atcaataagc ttaatatcat ataaaaacaa atctgcacat
     4021 ttagcaactc tttcataatt ttctgacttt gcatagccac atgtatcaat tgctacatgg
     4081 attccctttg atttgcacat gtttattact ccacaaatga agtccatatc ttgagccatt
     4141 acttctccac ctgatagtgt tactccacca cctgattctt catagaaaat tctatccttc
     4201 tctatctctt taaccaagtc tcttacagaa tactcttgac caacaatctc tcttgcattg
     4261 tttacacaat agtctaagca agtttcacaa aactcacact tatcttggtc taggcatatt
     4321 tttgtttcac ctttgtaaat tgccttatga gggcatatat ttatacatgc ttcacatttt
     4381 gaacatcttt cttcattata caaaacttgt tttgtatatg tttgactctc tggattatga
     4441 caccaaacac attcaagtgg acacccttta aaaaatactg tacttcttat ccctggtcca
     4501 tcatgtatgc tacatttttg taagtttata actaatggat tcatgatttt tcacccctaa
     4561 ataatatcca ttccattttt tcatttttat gtaatttatc ttcatagaat atacctatga
     4621 aaagcatttc tacaattaca taatactata tttgttatta ttttgtctat atatttttag
     4681 taatattaaa aaattttatt ttattatgat atttatattg tttttgtaaa ttaaatttaa
     4741 atatttagag ataagctatt atagaatttg cttagtttaa gttaaatttt aaaatattca
     4801 aaaaggggct agagtctgga aatttttatt taaagaatac acataaaaat ttctagactc
     4861 tcatcattta aaagaagtat tctaaattta ttatttataa ttaaatatta tctgaaaaaa
     4921 tggtattttt atacctttgt ttgttatgca taattttact tagaacataa gctttatata
     4981 aaataattaa aaatatacct aattaaaata agtaaagaaa gagctatcat agaacaaatt
     5041 taatttgctc atgtgacagc tcctttttag atgatgcttt tttagattta tactataagg
     5101 tacataaaac taagcttatt aaaaatgtta taatttctat tgctatcata gaccatatca
     5161 atccatcaag ttcataaaag taattaagct ttattaaata gcttctttat tataatttct
     5221 ttatttcata taatataaat agtattaaaa tttttatggg agttaaagta agcatgtatt
     5281 attacttaga tatttttata atcaacatct agtaatttac ctttttggtg aaaatgatta
     5341 ataagttttt taggataatt cattattaat tcatctggaa aattaatttc ctttaagata
     5401 ttttcagaat actcatagtt acctatgtca aaacaaatgt gagcatctga agttagtatg
     5461 attttagcac catatttttt gcaaagtgta gcaatatact tacagttatc cgagctacca
     5521 tttcttgaca cgcctttaat agaacaatta tttatttcta ttaagatatt cttttctata
     5581 gctaatttaa tgattaattc atagtcgatt gggtagtttg agtttccaag gtgtcctaat
     5641 atttcaatat tatcatgctt atttattgca tttattaaag ctttagtatt attctctaat
     5701 gtatttggag aaaacacagc ttcgtgaaat gacaagataa gataatctaa ccgaggaatt
     5761 acaaaaggct ctaggtctat gtttccttta atatctagta tatttgcttc acagcctctt
     5821 aaaattataa tgttatttat gattcttggg atattttgaa tgttgtgaaa gtaccatttg
     5881 tggggagaac caggcatctt aggaccatgt tcagatgtac ctagtatctt aattccattt
     5941 tctttacaat aattaatatt ttctagtaaa gtgctatagc catgaccact gactatagag
     6001 tgggtgtgta aatctgataa aaaattcata taaattcctc ctctataaaa gatgagggta
     6061 acgtaattta tattataaaa cgaataggaa agaaaatcaa tagttacata attagacaaa
     6121 aatattaaaa tattgtagaa ttaagcttaa taataaatgt ttaacagtat tactgcttat
     6181 aggggaggtt aactatgaca caaaagaagc tatcaaggaa agaaaaagtt ttaatttgct
     6241 tgaaaaggtt aacagaagaa cacttgaact ttaataatgg cattagattt ggatttgatg
     6301 cagaatatat agctactgaa gtaggtataa atagaagtaa tgctagtaag gaattaaatc
     6361 aattaataaa agaaagtatt attataaaga taaagggtaa gcctgttcag tacttatata
     6421 aagaaagtat tgagagtatt ttaggaaaga aactaaataa agggatattt gataatatta
     6481 actctataaa cgaatatagt gaaaatgatt ctatcaaaga tgagtatatt ttagagagta
     6541 taaaaaaaga gaacaatatt tttaatttag taggatgtga tggaagttta aggtcccaaa
     6601 tagaacaggc taaagcagca gtaatatatc cacctaaggg gttgagtaca ttaataatag
     6661 gacctacagg agttggtaaa tctatatttg cagaatatat gtataagtat agtttaacta
     6721 taaaagaaga tagtgataat gcaccattta tcatatttaa ctgtgctgat tattctgata
     6781 atcagcaatt attattagcg cagctttttg gatatgtaaa aggagcattt actggagctg
     6841 ataaagataa gtatggtatt gtacatgaag ctaataatgg agtattgttt ttagatgaag
     6901 ttcatagatt atctgcagag ggacaagaaa tgctatttct gcttatggat aaagggatat
     6961 atagaaggtt aggagaggct aataaggtac acgagtgtag agttatgata attgcagcca
     7021 ctacagaaga cccagaaaca gcaatgttgg aaacgttttt aagaagaata cctgtaacta
     7081 taaagattcc gtctttagag gaaagaggct ttcaagagag aatgaagctt atatgttatt
     7141 tttttaaaga agagagtgtt aggattggtg ttaagttaaa ggtttcaaaa gaagttatta
     7201 aggcatttat tttatataag tgtaagggaa acataggcca attaaaaagc gatatacaat
     7261 taatttgtgc aagagctttt ttagattata tgacttataa aagggaatgt gtatatgtaa
     7321 ggctctctct tctaccaaat aatattagag aatcattata tggaaataat agaaaagagg
     7381 agtttgtaca aaatttcagt tttattggaa aagatgatat tattttttta ccagaagaaa
     7441 aggattttga aagtgaaaat ttattaatag aaaataaaaa atatgattta gatttttatg
     7501 gaatgataaa agaaacttgg aataaacttc aacaagaagg tattaaagag tcacaaatta
     7561 gaagtgttat agatggagac atacagaagt attcctataa tttaatgaat acttttatat
     7621 ataatagtag taatcaaaca gcttataaca acattgttaa tgattcaatt gcaactactg
     7681 taaaatatat tttacaaaag catgataaat ggagcaaaag agaaggatta gataaattaa
     7741 taaaagctat agctttacat attcagaatt taattgaaag aataaatatt ggaaatatag
     7801 taaagcatcc taataaagat gaaattatga aggaaagagc atatgaatat aacatagcaa
     7861 aagaaatatt atctaacatg tcattaatgt atggagtaga gtttccagaa gatgaagcaa
     7921 tttttttagc aacatttctt tatttatcgt atgtgggatt aaatgaggag agtatagcaa
     7981 tattagtcat aatgcatggt gaatctacag catctagcat ggtgaatgta gccaatactt
     8041 tactggattg taaccatgca gtggcaatta atatgggatt agaagataga gttcaggata
     8101 ttctagtaca agctgtagaa atagccaata aaattgataa aggaaaagga attttaattt
     8161 taactgatat ggggtctatt ttaacatttg ctaaagttat tcgtgatgct acaggaaaag
     8221 aagtaaaagc aatagatatg gtaagtacac ctattgtaat agaagcaact agaaaggctc
     8281 taactccaga aatgacatta gagaaattat attttaatat tatagaatct ataaagaaac
     8341 attatggaga tatggaagtt acatatgctg aaaaagataa tggtggtaga tattttgata
     8401 gattactaat tgataatatt agtaaaactt tgactttttt aaatggggaa aaggcttatt
     8461 ttatattaaa agaagtaatt aatagaatta gtgaacacta ttctttagta attcaagatg
     8521 aacttttagt aaagtttata tttcattgtt catgtatgat tgaaagagta ataataaaag
     8581 agtctttagt ttataaatca tatgaagaaa gaattagtag aaataaagaa ttatatttag
     8641 ttataaaaga aagttttaag ctagtagaag agacattcga tataattatt tcagatatgg
     8701 aatatgcaaa tatattagat atatttgaat cacaatatga tacagataaa ttcaaaagtt
     8761 gatacaaaat ttaaaaatag ccaaaaggct atcttttttt tacttttttt gatacagatt
     8821 tgatacaggt tgaaaaaatc ttttgataca tatcgaaata ataagttttt aaaaaaaatt
     8881 gagtaaaaag ttttgattaa atcctgtaaa atagccattt gttattgata cacataagtt
     8941 ggcacggcaa ttgcttatat aaaaagtgac aagaaaagtg aggtgaaatt ggtgatagga
     9001 ataatagtta tgactcatgg gagttttagt gaagaaatta taaaaagctg tgaacttatt
     9061 gcaggaccag cagaaagaac agcagcaatt aaactaaata ggaatgataa tattgaagac
     9121 ttaaataaaa attttgtaga aaagttaaat gaattagatg aaggtgacgg agttttagta
     9181 ttagctgacc tattaggagg ttcgccaagt aatgttgcaa gtttaaacct taaaaaaggt
     9241 ggcaagtttc atgcacttac tggagtaaat cttccaatgc tacttgaagc tttaattaat
     9301 agagagggaa aaagtttaga agagttagca gaagcatgta ttgaagcagg acaaactggt
     9361 ataaacaata ttaataaagt tcttagctca atgtaataga aaaaggagtt tgattaaaaa
     9421 tgtctttagt aaaatattgc agatgtgatg atagattaat acatggtcaa gtgatatata
     9481 aatgggttaa gcatctaggt gtaaaaaaga tagtagtagt agatgatgaa acaaccaatg
     9541 atgttattgc taaaggtttg ataaaaatgg cagcaccaaa gaatattgat ttatcaatat
     9601 taactgtaag tgaatcaagg agatattttt ataataatca agctgatgat aacgtttttg
     9661 tattaattaa aaatttagat actgcaaata gaatgataga agaagggatt aatatcaaaa
     9721 aacttattgt tggaagaata cctacaggaa taggaaaaaa gaaaatatct caaaatgtat
     9781 acattaataa aaaagagttt cttttaatag atgaatttat taaaaaaaat attaatgtat
     9841 caattcaaat ggtacctgat gaagaagaag tagaattaaa ccacaatatt aatcgctata
     9901 aaggagagtt tatttagcaa tgaataaata taaggtaatc atactgtgcc atggaacttg
     9961 gggaaaagta ttagtagaag aaacaaataa aaactttgga ttgtcaaatc aatatgaagt
    10021 tttatcacta agttctgaaa aagatgtctc tgtatttatg aaagaggttg aagaagtagt
    10081 agataaagaa gatgtgaaaa ttataatatc agatttatat ggaggatcta catcaagtgt
    10141 tgcaatagct gttgctataa gaagaggaat agatgcaata aatggactta gcttacagac
    10201 aattttaatc gtagatgaag aattaagcaa agaagataaa ttacatacat taccagaaag
    10261 aatcgtcaaa aaaaataata acctttgtgt ggatttaatt aaagaattta aaaaaatata
    10321 aattatgagg tgaatataat ggcaaaaata acattagtaa gagtagacca cagattaatt
    10381 catggacaag ttataacaaa atgggtaaaa atcgctcaag ctcaaaaaat aataatagtt
    10441 gatgacttct taggtcaaga tgaatttatg gctgacatat ataaaatggc agcgccatca
    10501 ggagtagaag tagttatttt aacagcagaa gatgcaggac aagcattcca aaacaacact
    10561 ttaggagata aaaatatatt tattctattt aagaatgttg atatggcaaa taaggcttat
    10621 aaagctggac ttaaatatga aaagattcaa ttaggaggta ttccaaatga agcaggaaag
    10681 aaaatggtgt ttacagctgt ttcattagga aatgaggatg tagaacaact taatgaactt
    10741 aatgaaaatg gagttgaaat agttttacaa gttataccag aggaatcatc tatgacgtat
    10801 gaaaatgcgt taaagaaatt taaataaaaa atataaatta ggaggaatta taaatggaat
    10861 ctgttttgat gttggcaatt gtaacaggat tatggtattg gtttgcagca ggtcttgcag
    10921 ggtataccct tttctcaaca ctaaagtctc ctttatttat aggttttagc cttggattac
    10981 tttggggtga tgtaacaact ggtatgattg ttggtgcaag tatagaaatg gtttatctag
    11041 gaatggtcgc agcaggggga aatattccat cagataaatg tctagctgct cttattgcaa
    11101 taccagtagc acttcaaact ggtgttaatg cagaagttgc agtatctatt gcggtacctt
    11161 taggtgttat aggagtatta gttaataact taagaagaac tggtaatgct gtattagttc
    11221 ataaggcaga taagtatgct gaagaaggta atacaaaagg catctggaga tgtgcaacac
    11281 tttattcttt aatatttggg tttgttttaa gatttccaat agtttttgta tgtaactttt
    11341 ttggagcaga tttagttcaa tcattattag atgttatacc acaatggtta atgaatggat
    11401 taacagtaat gggcggtata ttacctgcat taggttttgc aacaacaata tttactatag
    11461 gtaaaaataa attcttacca atgtttatta tagggttttt tatggttcaa tattttgaaa
    11521 tatcaattac tgcagctgca atatttggtg tttgtatagc tcttttaatt acctttatga
    11581 aagaagacaa aagggtaggt gaagtataat gcaaggaata aaaaataaag aagtcaaaaa
    11641 tggacaaggt ccaactgaag ttaacaagac aagagtactt acaaagaaag atgtaacaaa
    11701 aacataccta agatggtggt ggacagcaga attatctaac tcttttgaaa gaatgcaagc
    11761 attagcagtt tgtgcaagtt tcactccggc tttagaaaag ttatataaga gaaaagaaga
    11821 tttagtagat gcgttaaaga gacacttaca attttttaat actcaagcta tatggggagg
    11881 attaattcac ggtacagttt tagctatgga agaagagaaa gctactgaag gaaaaatccc
    11941 aggagaagtt atttcaggtg ttaagaatgg tcttatggga cctttggctg gaattggaga
    12001 tactttagat tttggtacat ttcaaacaat attcttagct ttaggtgctt catttggagc
    12061 agaaggaagt gttataggtg catttttccc aataatgttt agtatattac tattttgcga
    12121 aggttattac ttattccatt taggatattc tttaggtaga gattcaatta agaaaattct
    12181 atcaggtggt attgtcaata agataattga tggtgcttca attctaggaa tgtttatgat
    12241 gggagcatta tctgcaacta cagttaagct ttcaactccg ctatcatttg atattggtgg
    12301 taaagcaatt gttgttcaag atacattaaa tatgattgct ccaggtttat taccacttgg
    12361 tgttgtattc ttcgtatatt ggggaatgaa atacaagaag tggacaataa ctaagttatt
    12421 agtaatttta gttgtattag cattggtagg gtcattcatt ggaatattct aattcacagt
    12481 gattaaaata tagaatttaa ttttaggagg agaaagaaat gtcagaaaaa atgtatacag
    12541 aaaaggaata tttagaagga attagaggag caacaggaga aagagcagta tggttctatc
    12601 ttcttatgaa agaagctgaa aaattaggag ttaatccaga taatatatgt aaagaagcaa
    12661 tatatggatt tggtaaaatg agaggtcaaa aatataacgt agcagatact ccaggaaaaa
    12721 tggcagaaat gttatataac agtaaaggac aaaaagtgtt tgaaatggaa ttagtagaaa
    12781 atacagatga gaatggagtt ttaaaattcc atcactgtcc attagatgcc gcttggaaag
    12841 aatatggatt aactaaagaa gaaagaaaag aaatttgtag attagcttgt tacggcgatt
    12901 atggaagagt tgactgtgca caaggcgtta agttagagtt tgctcaaaaa tgtgctcatg
    12961 atgatgaagt ttgtgaatta gtgtttacaa gaaaataatt tttaatgcta aaagcctgtt
    13021 ctttaaggac aggctttaaa aagatagaaa gtaggttgat ttatggatat aaacagaagt
    13081 gtagataata gtttaaatag aactatagaa tttctaaaag aattgataaa aattgattca
    13141 caacaaggtg aacctatttc acaatgtcct tttggtatag gacctaaaaa atctttagat
    13201 aaaactttag attactgtgc tagtttaggt tttagtgtta aaaatattga taattatatt
    13261 ggatatgcag aagttggaga gggagaggaa ttaataggga ttccaatgca tttagatata
    13321 gttccaccag gagaggggtg gagtgtagac ccattttcgg gagcagtaat tgacaatatt
    13381 atttatggaa gaggtgtcat agataataag ggggctgtat ctatgcttat acatgtattg
    13441 aaaaacatag aggatatgta tcctacaatt aataaaagaa taagattaat ttttggtaca
    13501 aatgaggaga caggtatgga atgtatcaaa tattacttat ataaaggtga agaaattcca
    13561 agtatgggat ttactccaga tgctatgtat ccagtggtta atggagaaaa aggaagggtt
    13621 catataagga tagaaaaaga aattaaaatt gacaaaagta aaccttacat aatagtaagg
    13681 ggaggaacta aagaaaatgt tgttccgtat cattgtactg caaaaattat taatggaatt
    13741 atttcagagt taactactaa aggagtagct gtacatgcaa gtaatcctga aaagggagaa
    13801 aatgctatat ctaaaatggt aataaagata gttgaagata atatggattt tcaacataga
    13861 gaagatatag aattagtttc aaaatacctt tgtagtgatt attatggaga tgcattgggg
    13921 attaatcaat atgatgaggt attcaaaaac actaccttaa atttaggaat attaaaagtg
    13981 aatgaggaaa aaatcgtatg tgagttagat ataaggtatg gtaaaaatat agttttaaac
    14041 aatataatag atagattcaa aaaggttttt tgtaatggtt ggaaaatagg ggttattgct
    14101 cataaagatt tacattatgt ggatgagagt aacttagtac ttaaaaaatt attagaagcc
    14161 tatgaagaag taacagatga aaatggatat acaattgcta tgggtggtgg cacatatgca
    14221 agttggttta aagatatggt ggcgtttgga cctaagttct tagcgtataa aactggtggt
    14281 catggagtag atgaaagagt accaataaat catatacgaa agaatatgga gatatatact
    14341 ttggctttaa taaaactatt agaattgtaa aagaagaaga gactttatgt gcaaatttac
    14401 agatatctta aaaagagaga taaaacgagt tcaaccttat ttaaatgttc aacaccatca
    14461 aatggggctg tctcaaaata gagataagcc tcttacttat tgttgaaatg atgaaaatat
    14521 attttacttc atacaaaaaa gagtgcctta tgaagtaaaa agttaatttt gagacaccct
    14581 catttgatga tgttgaagag aaaataagta tgaaattatt aaaaaagatg attttataaa
    14641 agttaaggtt gaaaagaaaa gttaattaaa tggaattaga taaatggaga aggatgtata
    14701 ctatctaata aaataacaaa agagtgttat aaagttggtt atatgattag agaaaagtct
    14761 atcgatattt atccagatag tggatgggga tttatgactt gggatgaaaa tggagaataa
    14821 taaaaattat agtttataca gtttaataag atatatgtac aagcagttaa tctaaatgat
    14881 tgactgcttt tcttttatag atttttatat aatagacaaa taccaataca tgatagttgc
    14941 ctgttataat tagaggaata aatacgaata ataactttat aaaaggatta aatggaaaat
    15001 cgaagagtta tattcagtat tttaaaaaga atatgaaatg agaaatgaaa ataaggtgtt
    15061 tatattaaaa gaggttttac acaaaatatt taaaaacgtg ataaataaaa actgtattta
    15121 gatagattaa ctattagttt tatataatat ggattttatt tacaaagttt acatatttta
    15181 caaaggagat taaagataaa tttaaatata tattttatat acttgtttat ttgtttctct
    15241 gtggttgatt ttttcgtcaa aagtcaactt caaatcgctt gttagcttta catgtttaag
    15301 gatataatta tacataagga gggattgaca tgaatgaatt aaacatcgca aaaacgctga
    15361 ttttaaagcg taaagaaaaa ggaattacac aagatgaact tgcaaattat ataggcgttt
    15421 ctaaagcttc tgtatctaaa tgggaaactg gacaaagcta tcctgacata acctttttac
    15481 cgcaactcgc gacatatttc aacattaccg ttgatgagtt aatatgttat gaacctcaaa
    15541 tgatgaagga agatattaat aaactgtata ataaactgtg caaagacttt acggttaaac
    15601 catttgatga agtcatggat gaaataagag aaatcataaa aaaatattat tcatgttttc
    15661 ctttgatttt tcggatgggt cttttaatag ttaatcacta tgatattgta gatgaaaaaa
    15721 agcgtgaact attaattagt gaagcattag aaatatttat tcgtatacag gaaacttgta
    15781 atgacataga tatttgccgt caagcaaaaa gtatggaggc aacttgttat atattgttaa
    15841 atcaacccat acaagtgatt gatttattgc aaaacagcaa ttttcccatg ataaatgaat
    15901 ctatacttct tgcacagggg caaatgatga atggccaaat ggatgaagca agagaaacat
    15961 ttcaacttgg tgcatatcag aaccttatat cattagtcca aaatctagtg ggtattttgc
    16021 aaaatgcaga taaattacaa atgaaggaaa tagaaagacg tattctagca atttcagata
    16081 tatttgagct tgatacatta tctcctgcta ttatgctttc ttcatattta acacaggcac
    16141 aaattaattt aatacatggt gataatgaag gagctattaa atcattacga aaatatgtag
    16201 atttagcaac acgtgatata tacccaatta taatacatgg ggatgacttt tttaataaac
    16261 ttgacagatg gatttctgaa attggaacag gtattacacg tgatgataca atcgtaaaag
    16321 caggtattgt tgctgctatt aaaaataatc caatgttttc tgtattaagt gaaaacaaag
    16381 aatacaaatt tcttatagaa aaattatctt tattggagga ataagattat ggaaaatttg
    16441 atggaatact taccactttt ggttcctgtt attattttag atttaatact tataattact
    16501 gcacttgttc atgtgttgag acatccaaat tataaaattg gaaataaggc aatatggatt
    16561 attgtagtac tgtttattag tctaatagga ccaattttgt attttacaat aggaagaggt
    16621 gaagaatgat gaatattgtc tcaataaatg gattatcaaa aggttttggt aatcgaaaga
    16681 taatagataa tttaaatttt actgtaccag aagggtcagt ctttgggttt gtaggtaaga
    16741 atggtgcagg taagactaca acaatgaaaa tggtacttgg gttacttaaa ccagatagtg
    16801 gaactataga tgtttgtggt gagaaagtga cctatgggaa aacaagttct aatcgtcatg
    16861 taggttatct gcctgatgtt ccagagtttt acaattatat gagaccactt gaatatcttt
    16921 ctctttgtgg tgaaattacg ggactttcaa aaaaagaaat acagataagg agtgaagagt
    16981 tgttatcact tgtgggtctt agaaatgaaa agagacgaat aggtgggttt tctcgtggaa
    17041 tgaagcaacg acttggtata gctcaagctt tgctttcacg ccccaaatta cttatttgtg
    17101 atgaaccaac aagtgcactt gaccctgttg gtcgaaaaga aatattagat attatgctta
    17161 aaatcaaaga ctcaacaact gtaatatttt caacacatat actatcagat gttgaaagaa
    17221 tctgtgacca tgttgctata ttaaacaaag gaagcattgc ccttagtggt acactttcag
    17281 aaataaaaag tatgcacgga aaggatagac ttttactgga gtttgcaagt aatgatgaaa
    17341 ttcaaaagtt taagtctagt gatggtatta aatccttact aaaagattca gaagaaacta
    17401 atatggaaat tgttttgcat ggtaaagata taaaagcaat acaaaaaaca gttatatcta
    17461 cactcgcaga aatgaatctt tgtccagtaa agatggaact aatagaatta tcacttgaaa
    17521 atttattttt ggaggtagta aagtgagaga atacatagca tttacaaaaa aagaatttaa
    17581 agagaattta agaaattata aattatttag tttaattatt ttatttctaa tatttggtat
    17641 aagtagtcct atttctgcaa aatttatgcc agacttgatt gctcattttg caccgacttt
    17701 aaaagttact gctgcaccaa ctgctttaga ttcttggaca caatttttta gcaatatctc
    17761 tggtcttggt atgagtttaa ctctaattat tttttgtaat attttatcaa atgaatattc
    17821 taaagggaca cttattatta tgctgactaa aggtttatca cgttcatcta tagtattgtc
    17881 taaattctct gttacggtga ttattatgac aattggtttt tggttgagtt ttttatttgc
    17941 ttatggctat acaatatatt tttggccaac tgcaaatctt aatcatatta tattttcagc
    18001 atttaattta tggcttatag gaattatgta tattagtgta ttaattcttg ggtgtgtact
    18061 atttagacca gcatttgcca gtgttttact tgtattggtg gcaactcttt tattgagttt
    18121 aatttctatt cctaaacaga ttgcacctta tactcctaat ttcttgatgt caaaaaatat
    18181 agacttgatt tcagggaaag tggcaatgcc agaatttatt attccaatta ttataacagt
    18241 tcttatttct gtagtatgtt tactagcagc tgttatttta tttaataaaa aaccagttta
    18301 ataatacctt tatcacaagg taaagtaatc ttatgttgtt aaaaactaca cctaaaaatc
    18361 aaaaagactt ggacttaagt ttcaagtctt tttgattttt aatatataga attaagtgta
    18421 gaaaataata caaataatat tatttgtatc aaataaatat aagaatattt aattatgtta
    18481 aaatatacta aaaattactt taaaagtgat aaaattagta taagtaaata ttttaaaggg
    18541 gtgtagatat ggaaaagtca aaagtatatt tttgtgattt atactcaaat tcgcaaaata
    18601 aaaacgtacc aaacaatgta agaagattat ttgataaagc aggattcaaa gatttaatag
    18661 aaaagaatga ccaagttgca attaaacttc actttggaga aaagggaaat actacatata
    18721 tgagtcctgt ggcagttaga caagtggttg ataaggttaa agactgcgaa ggaaagccat
    18781 ttttaacaga tacaaataca ctgtatacag ggagtagaac aaattctgta gaccatctta
    18841 caactgctat agaaaatgga tttgcttatg cagttgtaaa tgcaccagta ataattgcag
    18901 atggattata tagtagaaat tatgagaatg taaaaataga caaaaaacat tttgaaagtg
    18961 taaaaatagg tggagaaatt tataattctt ctgcaatgat agtaatgagt cattttaaag
    19021 gtcatgaagc agcaggtttt ggaggagcat taaaaaactt agctatggga tgtgcaagtg
    19081 cagcaggaaa acaaatgcaa cattctgatg ttactccagt agtaaaggaa aagaagtgtg
    19141 taggttgtgg aaagtgtgtt aattcttgtc ctacaaaagc tataagtata gtggataaaa
    19201 aagcagttat agattctgat gtatgttatg gatgtggaga atgtccaact gtctgcccaa
    19261 ctagagcagt aactatacaa tgggaaagtg actcagatgt gtttgtcgaa aagatggcag
    19321 aatacgctta tggatcagta tcaaataaaa aagacaaggt agggtatata acttttgtta
    19381 tgaatgtaac tccactttgt gattgtgtac cttggtcagg tagaccaatt gcccatgata
    19441 taggaatatt agcatcaaca gaccctgtag ctattgaaca agcatgctat gatttgattt
    19501 gtaaggaaat gggtcatgat gttttcaaac atgaacatcc tcatgtaaat ggaactagaa
    19561 taatagacta tgcatgtgaa atgggtatgg gaagtaaaga atatgaatta ataaaattat
    19621 aagaatagta gcacttaaat gcatataatt attgtaagag aaatctaaaa gtatcaaagg
    19681 agtatgcatt tatgaaaatt tactatgaga aggaaaacag caagaaggct aatgtaaaca
    19741 atattccaag tgtatttgcg tttaattttc taagaaactt tttgactagc aaagagtatg
    19801 aggaaactag aaaagaatat tttataaata atctaacaaa aagtcaggtt aatcaagcca
    19861 tgaaggattt aaaatggcta tttagagaat atgaaggctt agaggttatt acaatggaaa
    19921 gcttggatgg agttaagaca aggatagttt tataaaccag taagaattat attctaataa
    19981 aaaatactgt tttaataata atatgtataa tccatatact aatattattt tgaagcagta
    20041 tttttttgtg tatagtaaac ttgtgtctaa aataaagttg
//
LOCUS       DAEORZ010000067        20058 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEORZ010000067 DAEORZ010000000
VERSION     DAEORZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.067"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            77..1261
                     /locus_tag="KRH39_002146"
     CDS             77..1261
                     /locus_tag="KRH39_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG8512046.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGSLNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVELVVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGLNQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEHPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            1368..1556
                     /locus_tag="KRH39_002147"
     CDS             1368..1556
                     /locus_tag="KRH39_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG8512047.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            1706..2356
                     /locus_tag="KRH39_002148"
     CDS             1706..2356
                     /locus_tag="KRH39_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG8512048.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENIKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            2459..3397
                     /locus_tag="KRH39_002149"
     CDS             2459..3397
                     /locus_tag="KRH39_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG8512049.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            3423..4274
                     /locus_tag="KRH39_002150"
     CDS             3423..4274
                     /locus_tag="KRH39_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG8512050.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGTL
                     IKSDTIESKSERIKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(4559..5293)
                     /locus_tag="KRH39_002151"
     CDS             complement(4559..5293)
                     /locus_tag="KRH39_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG8512051.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGIDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            5419..6135
                     /locus_tag="KRH39_002152"
     CDS             5419..6135
                     /locus_tag="KRH39_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG8512052.1"
                     /translation="MKKFKLIGAIILVVFISVFISKERLLKKDKDPIVVAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            6269..6961
                     /locus_tag="KRH39_002153"
     CDS             6269..6961
                     /locus_tag="KRH39_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8512053.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKN
                     ENIHLMVLDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFNGANNEDKDVIEVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            6951..8132
                     /locus_tag="KRH39_002154"
     CDS             6951..8132
                     /locus_tag="KRH39_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG8512054.1"
                     /translation="MKNKSLLNHMFRPILDLIISCGLTSMLILTSMYIIKLLYFNSEI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHNGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            8414..8869
                     /locus_tag="KRH39_002155"
     CDS             8414..8869
                     /locus_tag="KRH39_002155"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBG8512055.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAVECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            8859..9584
                     /locus_tag="KRH39_002156"
     CDS             8859..9584
                     /locus_tag="KRH39_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBG8512056.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNIVELEGTNLTLESKDIYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            9556..11937
                     /locus_tag="KRH39_002157"
     CDS             9556..11937
                     /locus_tag="KRH39_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBG8512057.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDECVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKVLNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNITLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(12142..13371)
                     /locus_tag="KRH39_002158"
     CDS             complement(12142..13371)
                     /locus_tag="KRH39_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG8512058.1"
                     /translation="MNLNRNLEKYAELAIKVGINIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            13972..14886
                     /locus_tag="KRH39_002159"
     CDS             13972..14886
                     /locus_tag="KRH39_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512059.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEDLFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEEIQEENVILEKEESQDE"
     gene            14910..15542
                     /locus_tag="KRH39_002160"
     CDS             14910..15542
                     /locus_tag="KRH39_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBG8512060.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLDASQTISKCSDEVMCNPIGQALFLNEARVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKEE"
     gene            15918..16634
                     /gene="pyrF"
                     /locus_tag="KRH39_002161"
     CDS             15918..16634
                     /gene="pyrF"
                     /locus_tag="KRH39_002161"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBG8512061.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            16660..17703
                     /gene="carA"
                     /locus_tag="KRH39_002162"
     CDS             16660..17703
                     /gene="carA"
                     /locus_tag="KRH39_002162"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG8512062.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKETIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            17756..>20058
                     /gene="carB"
                     /locus_tag="KRH39_002163"
     CDS             17756..>20058
                     /gene="carB"
                     /locus_tag="KRH39_002163"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBG8512063.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDI"
BASE COUNT         8151 a         2107 c         3582 g         6218 t
ORIGIN      
        1 ttttattttt aggcttattt tctttatgta agaaaagcta ttagagaaat tatattgtaa
       61 ttaggatgtg gaataaatga aaaatttact tataagaaat gggacaataa tatctccaac
      121 agatggtatc aaatttgaaa aaagagatat cttggtttta catgggaaaa tatatgctat
      181 tggattgaac cttaaagaga aaataagaaa agaaaaatta cttacaaata aagagttcga
      241 gataataaat gccgataata tgtatatttc cccaggattt atagatgttc acacccattg
      301 ttataagaga aaattaccta caggtatgga ttctgattca attggaattg aaaaaggttc
      361 tacagttata tttgatgcag gtacagctgg ttctttaaac tactatgatt ttaaaaaaaa
      421 gtatatggat gagtgtaaaa cagaagtata ttctttttta aatgttacaa atgagggact
      481 aaatatattg aatgaatcaa cttctttaga tgttataaat tttgacaagg ttgagttagt
      541 tgtagaaaaa aatatagaaa agataaaggg cataaaagta aaagcgagta aatttcaatc
      601 taatgaaagt ggtatagata taattaagaa atcaaagcaa gttgctaaaa aattaaattt
      661 acccttagtg gtatatatag gagagtatcc atcatatata gatgaagtaa tgaatatttt
      721 gggaaaagga gatgtggtta caccagtata tggaaatagt acaaatggaa tccttaaaga
      781 atctggcaca ttaagaaaat cagtaataaa tgcaaagaac agaggagtat tgtttgatgt
      841 atgtcatgga ttgaaccaat ttgattttaa agtttttaaa aaagcaataa agcaaggttt
      901 agagccagac ctaataagta ctgatatgca tataaaaaac atgaaagacg taaactattc
      961 tcttttaaat gttataaata aaattatgga acttgggata tccttagaaa agtgtataga
     1021 aaaagtaact gaacacccaa caaagatttt cgatttacat ggaaataaag gcagaataaa
     1081 gattggtgga gaaggtgatt ttacaatatt cacaatggag gagtgtaatg atattataaa
     1141 agactacaat aataatgaac ttgttttaaa taaaaaatta agattgcaat atactgtaaa
     1201 atcatggatg aaaagcagtg aagtttatag acatggatac gaaaatacta taataaacta
     1261 aacaatagtg ataaaataat aaatatatgt ttaaattggt atatattgga taaatatcta
     1321 aggaaacaac aattaaaaaa taataatttt aggggaggta ttttagtatg aaagcatttg
     1381 ttgataagga tgtatgtgta gcttgtggag catgtatagg aacatgtcca gacgttttta
     1441 gtatgagcga tgaagggcat tcagtagcaa ttgaaggaga aataccagca gacttacaag
     1501 atttatgtaa agaagccgaa gaaggatgtc cagtttcagc tataacagtt aaatagagct
     1561 gtttaaattt atttttaaaa taaatatata agatttaatt gggtacttaa gctatagtat
     1621 tagaaatttc tattactata gctttttaaa atatataaac ttaaaatata agatattact
     1681 ttttacaaat atggaggact gaaatatgaa aaaaaattat gaaatagtat tattcgattt
     1741 agatggaaca cttacagacc ctaaagaagg aataacaaag tctattcagt attctttaaa
     1801 tagctttggc ataaaagaag atttggaaaa cttagaccaa tttataggac caccattaca
     1861 tgatactttt aaggagtatt ataaatttga agataaaaaa gctaaagaag ctgttgaaaa
     1921 atatagagaa tattttgctg ataaagggat atttgaaaat aaaatctatg agaatataaa
     1981 agagatatta gaaatgctct ataaaaatgg taaaatatta ttagtagcaa cttctaagcc
     2041 aacagttttt gcagaaacta tacttagata ttttgatata gatagatatt ttaaatatat
     2101 agcaggtagc aatcttgatg gtacaagagt gaataaaaat gaagttatac aatatgtact
     2161 agacctatgt aatgtaaaag ataaagataa agttattatg gttggagata gaaagtatga
     2221 cataattgga gccaaaaaaa taggtataga ttctattgga gtgttgtatg ggtatggttc
     2281 ttttgaagaa cttagtgaaa gtgaacctac ttatattgtc gaaaatgtag agtctataaa
     2341 agatattttg ttataaatat cttttataga caaaaatatt acaaaatggt gtcgcatatt
     2401 taaaaatatg atattatagt taaggaagtc atttaaaaaa gaggaggatg cacttaaaat
     2461 gaaatctatt ttaattatgg gtggaagcga cttcataggt tctgcacttg ctaagcgctt
     2521 gattaaatgt gggtatcaaa ttgatatact tacaaatggt aaaaaggaaa tagattataa
     2581 tggttttaaa gaacatctaa tttgtgatag aaaagttcga aaagatatgg aaaacattat
     2641 cacaggaaga aaatatgatt acatatatga tatgacagca tatacaaaag aagatgtaag
     2701 taatctgatt gattttataa gtatggataa cttaaaaaag tatatagtat tgtcagcagg
     2761 agcagtatat aaagatagtg gaagaaatat aaaagaagaa aatgagaaag gtgaaaatga
     2821 aaactgggga aaatatgggt taaataaaaa agaggctgaa gattttatta taaatagccc
     2881 tataccatat ataattatta gaccaactta tatatatggt gaaaataata atttatatag
     2941 agagtattat ttctttgaaa aaatagaaaa aaatgaaaag ataccagtac caaaaggaaa
     3001 acaagttagt aatcaattta tatatattgg ggatttagta aaggtattgg aaagcataat
     3061 gaaaaatcct catgtaagag aggcctataa tgtgacaaat ccacagttaa tttcttggga
     3121 tgatcttata tatacatgtg gagaaattat tggcaaagaa cctataataa aatatgtaga
     3181 tatggaaaaa gtagagttta gagaaagaac gtatttccca tttagaaata tagactttaa
     3241 tcttgatata aataagctta ttgaacatgg tctttatata ccaaatgtgt tactaaaaga
     3301 aggtcttaca gcaacatata aatggttttc agcaaataag ccaaaaatgc atgacagaaa
     3361 aatgaacaaa gttgagtctg taatgcaaat agtttaaaaa aataaataag taggtagata
     3421 ttttgaactc aaaaattaag tattttttaa aattttttat cttaatattt attttgattt
     3481 ttatatactc agttgcgata gagccgagtc tattaattgt gaaaaaatat aatataagtg
     3541 aaaatggaac attgataaaa agtgatacaa ttgagagtaa atctgaaagg ataaaagtag
     3601 ttcaaatttc agatactcaa ataggtagtt tttattcaac taagaatctc aaaaaagtag
     3661 ctaataagat taacactttg aatccagata taatagtatt tactggtgat ttaattgatt
     3721 actccaataa aaatccaagt gtagatgaaa ttacaactat tttatcaagt atgaatgcta
     3781 ggttgggaaa attttcagta tttggcaatc atgattatat gtataaactt cctaggtatt
     3841 ataggcagat tatgaaaaat tccaacttca atcttttagt aaatgaaaat aaaaaaataa
     3901 aattaaaaga tgataaatat attaatatat taggtgttga tgaaatatta aatggaaatc
     3961 ctaatataaa atatctggag agtcaaatag ataataagaa ttttaattta ttgttagcac
     4021 atgaaccaga tttagtagat atgcttagta aagatacaat gaatttagtt ctatctgggc
     4081 atagtcatgg tggtcaaata agattaccta ttaagggagc attagttact cctccatatg
     4141 gaagaaaata tactaaaggg ttttatgata taaatggaaa tcatttatat gtgagttcgg
     4201 gtcttggaag tacaaagtta ccgtttagat tttttaatat accagagata atagaattta
     4261 atattataat ctaacataaa atatttagaa aatataattt tgctttttat aatatgtaaa
     4321 tgtatatact gtaaaaatgg gttaaaccag tagattgaat gaattaatag attaagttaa
     4381 tatataaaat acttaagtta taattaatgt attagataaa gtaaatacta ttaagaacta
     4441 ttaaaatata gattaaagat aataacatat aataaataaa aaagtaagat tgtgtagatt
     4501 atataaagat tatttacaat taatacaatc ttgctttttt atttaaaaat tttaattatt
     4561 atttaaaatt ttttatcttc tctttatccc atataaacaa tttctcagaa ttatttaaag
     4621 ctccaaatac atttctttta aaatcaggca tctctttatt taaattagca ataagatttt
     4681 ttatgtactc tctcctagta tgaccatttg cagggcatgg atttgtgata ataggatagt
     4741 tagaatcttt tgaaattttc ttagtcatat actcttctat gtaaatcata ggtcttataa
     4801 ctgttaaatc ctgtctatct aggtatgtct ttggggaaaa acaatttact ctaccctcat
     4861 aaaacataga cattaaaaat gtctcaattg catcatcttt atgatgacca agagctactt
     4921 tattacatcc aagtttttta gcattatcgt tcaaagcacc tcttcttaaa ttagcacata
     4981 atgcacaagg attcttttct tgtcttatat caaatacaat ttcttttatg tcagtttgaa
     5041 attcataaaa aggaacattt atttctttac acaatttatc tagtggagaa ttatcaatac
     5101 ctccggtatt taatgttatg gctatcaatt caaagttttg tggagaaaat ctttgataac
     5161 ttttaagtat atgaagtaga gttaaactat cttttccacc agaaagtcca accgcaattt
     5221 tatcattttc ttgtatcata tcaaaatctt gtatagcctt acgagcttta cttagaagtt
     5281 tttgtataga cattttatca ccatccttta aaaatattaa atttaagatt tatttaagat
     5341 ttacttataa aatacttaag tcttatactc taaaatcaac attatagaca aacataacaa
     5401 aaaggagaat atgtaattat gaaaaaattt aaattaatag gagccattat tttagtagtg
     5461 tttataagtg tatttatatc taaagagaga ttacttaaga aagataaaga tccaatagta
     5521 gttgctcaac atgattcaga acaagatgtt gaggcaaatt ctcaaaataa aaatcactca
     5581 aatagtatat caaatatatt attggtaaac aagacaaatg gaattagcaa aaactatact
     5641 cctgaaaata ttactaaggt aaacatacct tttgttgaag aagcaacaga ggaagaaaaa
     5701 caaatggcag gagaacctgc aaaggcagtt gaagatttag ttaagcaagc taattcagaa
     5761 ggaatacagt ttttagggtc ttcagcatac agatcttatg atactcaact ggatacttat
     5821 acaagaaggg taaaatctca ggggcgagaa aaggcagatg catatgtagc aaagcctggt
     5881 tacagtgagc atcaaactgg actttgtatt gatttaacaa atccagaaag atggtttgtt
     5941 ggaagtacaa aagaagctaa atggttggca gaaaatgctc acaagtttgg atttataata
     6001 agatatcctg aaggtaaaga agatataaca ggtacagcat atgaaccatg gcatattaga
     6061 tatgttggta aggatgctgc cgaggaaatt tattcaaaag gattaacttt agaagaatat
     6121 ttacaaaata aataatataa atgtgtaaat aatttgtatt atattggaaa tttagaaatg
     6181 aataaattta tagattacac taactaaaat atagtataat tttatatgag taaagttata
     6241 tacaaagatg aagttaggat gtgtaaaaat gaacaataca gttcttgttg tggatgatga
     6301 aaaggaaata agagacctta tagagatata tcttttaaat agtggatata atgtaattaa
     6361 agctgaagat ggaaaagagg ctctagatat tttaaaaaat gaaaatattc atttaatggt
     6421 attggatata atgatgccta aaatggatgg gatagaagtt tgtagaaaag ttagagaaag
     6481 tttgaatata ccaatactta tgttaagtgc aaaatcagaa gacatggata aaatacaagg
     6541 gataatgact ggtgcagatg attatttgac aaaaccattt aacccacttg aacttactgt
     6601 gagagtgaaa tcactgatta gaagagcata ttattttaat ggtgctaata atgaagataa
     6661 agatgtcata gaagttggag tagtaattat agataaggaa aaacatagtg taacagtagc
     6721 tggaaatgaa ataattttga catcaagaga atttgatata ttatatttac tagcaaacaa
     6781 taaaggcaga gtatatagta cagaagaaat atttgaaaag gtatggaaag agaaatatta
     6841 ccaatctaat aatactgtaa tggttcatat gagcagaata agggataaaa ttgaaaaata
     6901 tacagatgga gaaaaaataa ttcatactgt atggggagta ggatataaaa ttgaaaaata
     6961 aaagtttatt aaatcatatg tttcgaccta tattagatct tataataagc tgtggtttaa
     7021 cttcaatgct tattttaaca agtatgtata taataaaatt attatatttc aatagtgaga
     7081 tatctgtaat taatcaactt gtatatcagt taagagattt cagaagtctt ataggagaga
     7141 gtttatttta tccattttta tttacaatca catcattagc aatttattcc atgatatcgt
     7201 acaagagaaa taagcaaatt gtagatttta taagaaagac taatagaaaa aatcaaaaat
     7261 tagaaatcat taaaagtgct gtaaatttaa tggatgaagg taacttagaa aaaagtatag
     7321 atattgagta tgacttagat atattaaagg ataaagaaaa ggatgatatt gatgaattag
     7381 cacataaaat caataatata gtccatcaac ttagaaatat aactattgaa gagagacaag
     7441 ctcagcagac aaaaacagat ttaataacaa atgtgtcaca tgacttaagg acacctttga
     7501 catccataat gggttattta ggagtgatag aagaaggtaa gtataaagat gaagttcaga
     7561 tgatgtatta tgttgatatt gcatatgaaa aatcgaaaaa tttaaatgtt cttataaatg
     7621 atttgtttga attgactaaa atgcaaaata atacaataaa attaaacaaa atagaaataa
     7681 atttagtaga gttattaagt caagtagtat ctcaatttga aatttatttt aaacaagaat
     7741 ttatggttag tagaattaat tttaatgaag aaaaattaat tgtaaaagct gatccaaata
     7801 aattggtaag agcatttgaa aatcttataa ctaatgctgt gaaatatgga aaagatggtc
     7861 attatgtaga tatagtcaca gagaaaaagg aagatatggc ggttgtaaaa gtgataaatt
     7921 atggagaacc aattcctgtg ttagatttac caaatatatt tgatagattt tatagagttg
     7981 aaaaatctag aaatagaaat gttggaggtt ctggattggg tcttgcgatt acaaaaaata
     8041 ttgtaaattt acataatgga gaaattactg tttcaagcga taaatatcaa actgtatttg
     8101 aggtacaact gccaataata caatcagctt agttataaaa taaaagtacg attatgtgat
     8161 ataaatatta taacaataaa gtgtttacaa aaataaaaaa gtaatatata atttaagtca
     8221 gtttaataag atacttatca agagttaggg gagagagtta gctcagtgat cctataccaa
     8281 ccttttatca ggtggtcctg ctaacaacga tgagatgact tgaaataata tactcttctt
     8341 atcaataata gataggaaga gtttttttta gaattaatgt aacaaatttt aaagtataat
     8401 ggaggattaa ataatggaaa aagtagaaag ttttaagcta gaccatacaa aagtaaaagc
     8461 tccttttgta agaaagtgta gtgtacttga tggagtaaag ggagataaag taactaaatt
     8521 tgatttgaga tttttacagc caaatgtaga aagttttgga actgctgcta tgcacggttt
     8581 agagcatctt ttagctactt atttaagaga tacattagat ggtgtaatag acttatctcc
     8641 aatgggatgt agaacaggat tttatttaat actatgggga gatgtagatg ccaaaacagt
     8701 taagattggt ttagaagaag cattaaaaaa agttcttgaa tcagataaaa tgccagcagc
     8761 tacagctgta gaatgtggaa actacagaga cttatcttta tttggggcaa aggaatatgc
     8821 aaaagatgtt ttagataaag gattttcttt aaatatatat ggagaataat ttaattggaa
     8881 gagaagatat aagcataaat caaagtgaag ttttaagata tttacaatac agaggtcaag
     8941 agatagataa agaccttaaa tatactattg atgaatgtgt agctataact aaaaataaaa
     9001 taaatcctag atatttatct aggatttatc ctattagaat aaataaagat aatattgtag
     9061 aattagaggg aacaaactta accttagaga gtaaagatat atatgaactt ttaaaagaat
     9121 gtaatgagtg tgtaattatg gcagctacta tagggataga tatagaaaaa gaaataagac
     9181 gacactctta ttcaaattta acaaaaggac taataattga ttcatgtgga acaacagcaa
     9241 tagaagaagt gtgtgacatt gttcaaaata atttagaaaa ggttttttta aagcaagata
     9301 aatttataac tatgcgatat agcccaggat atggagattt accaatagaa aaaaatatag
     9361 atatattaaa cgttttgaat gctcaaaaac aaataggtct aacaattact tctaatggaa
     9421 ttatgatacc gagaaagtca gtagtggcta ttataggtat ttctaataca agaataaata
     9481 aatctaagaa agaaaagtcg tgtgaaaatt gtaaaaacta tagaaactgt acatatagaa
     9541 aaggagcgaa ctcttgtgga aataagacaa tatctgaaaa ataatatatt aatatttgat
     9601 ggtgcaatgg gaacaatgct tcaacaaaaa gggttaaagt taggtgaaaa tccagaagta
     9661 tttggtcttc aaaatccaga taaacttata gaaatacata cagcatactt agaggcaggt
     9721 tcaaatgtaa ttcttacaaa tacttttgga tgtaatgaac taaaattaga tagtaagtat
     9781 actgtagaag aagtgattga caatgctgtt ttggtggcta gaaaagctat agaaaatgta
     9841 gataatacta aaccaagata tgtagcttta gatattggtc ctataggaga aatgctagag
     9901 cctatgggta ctttaagttt tgataaggca tatgaaatat ttaaaagaca agtactacaa
     9961 ggcgttaagt ctggagttga tgtgatagta atagagacta tgatggattt atatgaagct
    10021 aaagtagctg tgttagcagc taaagaaaat tctgatttac ctattttctg tactatgaca
    10081 tttgatgaag gtggaagaag ttttactgga tgtatgccag aatgtatggt ggctactata
    10141 gaaggtctag gtgtagatgc aataggagtt aattgttcat taggacccaa acaactttta
    10201 ccaatagttg aaaaaatagc atcaagagct acagtccctg ttatggttca agcaaatgca
    10261 ggtcttccaa atatagttga tggagaggct atatatgatg ttgatgcaaa agaatttttt
    10321 gaaggggtta aaaagttcgt tgaggttggt gctactataa taggtggatg ttgtggtacg
    10381 aatccaagtt ttataaaaga aatatctgag aatataaatt ctgttacaaa gggatgtatc
    10441 gagaaaattg atgaatgtgt agtctgttca ccatcaaaat ttgtggaagt acaatcgcct
    10501 acagttgttg gagaaagatt gaatcccaca ggtagaaaat ctctacaaga agcattaaaa
    10561 aatgaaaatg ttgattatgc tataaattta ggcttagaac aagtaaatgc tggagcacaa
    10621 atacttggag tgaatgttgg tcttccagag attgatgaaa aaaaactaat gccaaaatta
    10681 ataagagaga ttcaagcagt agtagataca cctttacaag ttgattcttc caatgtagag
    10741 gccttagagc aaggacttag atattataat ggtagaacaa tagtaaactc tgtaaatggc
    10801 aaagaggagt ccttagaaag catattacca atagttaaaa agtatggttc atgtgtggtt
    10861 ggacttacac ttgatgaaaa ggggatacct aaaaaggctg aagaaagatt tgaaatagca
    10921 aaaaggatag ttaatagagc agtttcttat gggataaaac caaaagacat atttatagat
    10981 tgtttatcat taactgtttc agcacaacaa gaagaagcaa tagaaactat aaaagctata
    11041 acaatggtaa agacactagg agtaaaaact atattaggtg tttcaaatat ttcatttggt
    11101 cttccaaata ggaaagcact taatgcttct tttctaacat tagctttagg ggcagggctt
    11161 gatttagcaa ttataaatcc aaatgaatat agtatgatgg aagctatcaa ttcttttaaa
    11221 gtattaaata acacagataa aggatgtata aactatataa atcaatatag taatataaat
    11281 aattcaaaaa aatcggattc atctacaaag attgacaaag atttgccctt ggatatttta
    11341 gttgaaaggg gattaaaaga tgaagcaaaa aatataacac tgaatctttt aaaagagaag
    11401 gacgaaaatt atatattaga tgagatttta attcctgctc ttgataaagt aggaaaaaga
    11461 tatgatagtg gagatatatt tttaccacaa ttgattcaat cagcagaaac ggtaaaagtt
    11521 tcattaaata caataaagga aactttatta agtaaaagta gcaataatgt atcgaaagga
    11581 aaaataatag ttgctacagt aaaaggtgat atccatgata ttgggaaaaa tatagtaaaa
    11641 ataatgctag aaaattatgg atatgaggtt atagatttag gtaaagatgt ccctattgaa
    11701 gaagtagtag aagttgctaa aaagagaaat ataaaattag taggattaag tgcacttatg
    11761 actacgactg ttcaaagtat gaaagataca attcaggcac tgagagataa tgagataaat
    11821 gcaaaggtat ttgtaggagg tgcagttttg acagaagaat acgctgaaga aatgggtgct
    11881 gattattatt caaaggatgc aaagtctgct gttgaaatag caaagttaaa tttttagcat
    11941 gtaaattaaa ataaaggttt tactaaatag gatattttat atataatata aatataagtt
    12001 ttagatactt atgtttaaaa caataggaat taatctataa taatgagatt aattaatata
    12061 aaaaatattt atcatcaaca ttaaaaaggg ctgggaagtc tggtataact tccatcagcc
    12121 ctttttagtt aaaattatat gttaaaaagc ccaattacca tttttaaaaa tctgtatttg
    12181 ctttccatca taagtctcac caataatatc catatcagca gaaccaatca taaaatctac
    12241 atgtgttaaa ctgtcattta caccatgttc atctaattct tcatctttta aattgtttcc
    12301 accttccaga caagtcttat aagcagagcc aattgcaaag tggcaagaag cattttcatc
    12361 atatagagta ttataaaaaa ttatatctgt attggatatt ggtgagttgt atggaacaag
    12421 agcgacttct cctaaataat gagagccttc atccgtttca ataagttttt ctagtgtatc
    12481 tagtcccttt tcagcagaga aatctattat tttaccatct ttaaatgtta agaagaaatt
    12541 gtcaattata tttccaccat aaacaagagg ttttgtacta taaacagttc catttacttt
    12601 aaacttatgt ggcattccaa aaatctcttc agttggaata ttcggattaa aacttattcc
    12661 attagggtct ttggatgctc cacttagcca aacatgaccc tttggcaagt ctagagtcaa
    12721 atcagttttt gaggatttat attttaaagt tttaaagtta tttttattta gaaagtccat
    12781 tttagattta agattttcat tatgctcttc ccatgctttt atagggtctt gaccatctac
    12841 tcttgaacat ttaaatatag catcccacag tttttctatt gctttatctt ctgatagatt
    12901 tggaaacact ttttttgccc aatcttttgt tggcacagag actatactcc atttacattt
    12961 atcagttaga gtataagaac gccattcttt taaagctttt ccggatgcct tctgaaaatt
    13021 tgcaactctt tcagggtcaa catcttttaa taaatctgga ttttgggcat agactgataa
    13081 aaatgctcca ccttcttttg ctatatctac atattgttct gctgtccatt tggggaactc
    13141 atgaaatgct tcatctggag catataagta tttaataaga gtactttctt catcagacca
    13201 ctctatataa acatttttag caccacattt atatgcttct ttaagtgcat ttcttgcaaa
    13261 atcagcacat tctattggtg actttataag tagaatttga cctggctgta tgttaattcc
    13321 aacttttatt gctaattcag cgtatttttc taaatttcta tttaaattca tatttatcct
    13381 cctatatttt tgatattgaa taacttataa agtattataa cattacttat gtttttaata
    13441 ttgaacaact tataaaatat tataacttta cttagatttt gggtcatatt caaaatattt
    13501 atactttata gattgagaaa taaaaaaaat attttaatta aaacagatta tgaggataac
    13561 aaacttattt aagcagtatg ttttttgctg ataatagtat taaagtagaa ataaaaatac
    13621 ttttttagtg aaaagctata caagaatacc gaaaaaaact tataatataa taatgagata
    13681 gatatttata aatgaaaaaa aatttttctg gcaaagctta attaaaaagt taatagctag
    13741 tgttgttatt taaattagca tagaaaatca aataaaaaaa ttatgctaat gtatagacat
    13801 taataaggta gaatttaagc cttttgaatg ctatattaaa ctagagtaga tattcttatg
    13861 aatttcaaaa tagggcagga ggaaaaagga aataaaacta taaaaaatat aaagtattta
    13921 tataaattta tagagtttat acgtcagaag atttcagtta ggagggcata aatggagctt
    13981 tatgaagcca ttttttatag aaaatctgta agaaattttt ccagtaagaa gataaaaaag
    14041 ccattgatgg aagaaataaa aagaagttgt aaaaatttag aatacttaaa tgaagattta
    14101 aatattaaag cacatgttgt tgatagaggt catataatac attttttgat gggtaaggaa
    14161 tgtaaagtaa aggcacctca ttatatagta gttacatcag ataaaggtga ggactattta
    14221 cagaatatag gatttgctac agaaggtgta gttttgcgcc ttacaacatt gggaatagct
    14281 acttgctggt tggaaggaaa cttaaagaga gaagatatct tggaatttgt agatattggg
    14341 aataagaatt ttgaagaaga tgaggaagat gatttagatt atctagctgg aataaaaaca
    14401 gaagaagatg atgaaaacaa aatagaaaat ccttatataa taatagcatt tggatatcca
    14461 gcaaaaaatg aagacttgtt taggactaga tatgctagac ctgatagaaa gccagtaaaa
    14521 gatatgataa ataagataaa taggaataga aagtggaaag atatcttaga attgacaaga
    14581 agagctccat ctgtaaaaaa ttctcaaccg tggtatttcc ataaggatga acgaggtctt
    14641 catttatttg aaaagagacc taagaaacac tgtgaagata tgaataaggt tagcttaggt
    14701 gtagccttaa gacattttga tatagcttgt ataaaaaata aaatagatgt ttcatatgaa
    14761 aaacttccta taaggaataa aataggaaaa tcatatttta taactgtagt cgagcatgtg
    14821 aaaccagaag aagaaatcca agaagaaaat gtaattctag aaaaagagga atctcaagat
    14881 gaataaaatt ttggaagggg atattggata tgtataagtg tggaaaatgt aatcttagag
    14941 gctgttctaa tggaaatatg gataaattaa tgaagatatg tccaactaga gaaattgaac
    15001 tacaacaaaa agcaaagcaa ctttatgaag aagaagagaa tagaaaaata gcttataatg
    15061 ctggattagt tgaagcagag gggtattgtc aatatactag actaaaagag attatagagt
    15121 ttgctaaaaa gtgtggttat aaaaagattg gcatagcatt ttgtattggg ctaaaaaggg
    15181 aaatgcaaac agtacaaaaa atatttgaac atcatggatt tgaagtattt tctgttatat
    15241 gtaaaaatgg agcaatgcct aagagtacaa ttggactaga tgcatctcaa acaatatcaa
    15301 agtgttcaga tgaggtaatg tgcaatccaa taggacaagc tttattttta aatgaagcta
    15361 gggttgacct taatgtaatt cttggattat gtgttggtca cgatacttta gccatgaaat
    15421 atatggaagc tcctataaca actttagttg tcaaagatag agtaactggg cataatccag
    15481 ttgctgcaat atataatgca gaatcatatt ttgagaaaaa attgtttgat aaagaagaat
    15541 aattcaaaga cttattgaca taaaaagcaa ttagtggttt aattataaaa aagataaaac
    15601 tttaaattat cctagtgata cataaagtta tataaataaa gaaaaatatt acagctaaat
    15661 tccttaaagt agtccagaga gactaaaggg ttatagatag tgttagtata taacctctag
    15721 taaatcttct agaggttttt gatgagtata aaataaaaat ttaaatataa taaagtcttt
    15781 taaactagtc cagagagact gggaaagata tctcatagct aatgaataga gttatgtata
    15841 gataaaatcc ttgtgcctct tttggtgtga ggattttttt atgattattt aggctttatt
    15901 aaaaaggaga tgtaaaaatg ataaatggta aagaaaagtt gatagttgca attgatactg
    15961 acgaatttga taaagctaaa gaactaatag acagattaga agatagtgta gacatattta
    16021 aggtggggct agagcaatac gttgccacaa aaggtaaaac aatagattat cttaaagaaa
    16081 aaggaaaaaa aatattttta gaccttaaat tccatgatat accaaatact atgaaaagtg
    16141 cagtgagagc tgcagtgaga gataatgttt ggttaatgac aatacatgta tcagatatgg
    16201 aaggaatgag acaatgtgca ctaattgcta gggaagaagc agaaagatta aatatagaaa
    16261 agccattagt agttggagta acggttctaa cttcattaag caaccaagat ttacaggaca
    16321 taggatgtaa tatgacaaca gaagagttag ctataaagag agctaaactg gcaaaagaat
    16381 caggaataga tggggttgtt tgttcagcac aagaagtaga taaaatagta gaagcatgtg
    16441 gaactgattt tataacagtt tgtccaggaa taagacctaa atcagcagag gttggagacc
    16501 aaaaaagagt tgtaactcca tcagatgcaa taaaaaaagg tgctcattat ttagttgttg
    16561 gaagacctat aacaaaagca gaaaatccaa gagaatcagc tattaatatt gtaagagaaa
    16621 tagaaaatgc ttagataaaa tatttgggag gtagcttaca tgaaggggaa attaatttta
    16681 gaagatggaa cagtatttga aggaaaagca tttggatatc taaaggaaac tattggagaa
    16741 gttgtattta atacttcaat ggctgggtat ggagaactat tgacagatgc aacttattat
    16801 ggacaaatag ttactatgac ttatccactt ataggaaact atggaataaa tttagaagat
    16861 gttgaatcaa ataaggtgca agtaaaagga cttattgtaa gagaaaaaag tgataaccca
    16921 aataatttta gatgtgaaat ggatatagac acatacctaa agcaaaataa ggtaatagga
    16981 cttgaaggaa tagatacaag agcattaact aaaattatta gaaacaatgg aactaagaga
    17041 ggaattataa ctttagaaaa tgtatcatta caagatgttc agtctaaatt agaagagcat
    17101 tgtaacaaag attcagtaaa aaatgttaca agaaaagaaa ttgaaaatat aaaaggtact
    17161 ggtgctaagg ttgtagttat agactttggt gttaaaaata gtgtactaga gtctcttaaa
    17221 gcttgtaact gtgatataac tatagtacca gcaatgacta cagtagaaga aattttaaat
    17281 ataaatcctg acttaatctt attatctaat ggaccaggaa atccagagga tataaatgat
    17341 atagttgaaa atataaaaaa attaataggc aaaaagccat tgacaggaat aggtttagga
    17401 catcaaattt tagcattggc attaggagga aagacttcaa agcttgtatt tggacataga
    17461 ggaggcaatc acccagttaa atgtttagaa actggaaagg tatttataac ttctcaaaat
    17521 catggatata atgtttcaga aatgccagaa aatacagagg taactcacat aaatttaaat
    17581 gacaatacta tagaaggaat gagacataag gaattaccaa tatatagtgt tcaatacatt
    17641 ccagaataca caacaaaaga agatttagat tgtatattta ataaattctt agcattaata
    17701 tagatttagt attatttaca gatagtaaaa accatttaga taaggagaaa aatttatgcc
    17761 taaattagat agtataaaga aaactttagt attagggtca ggaccaataa taataggtca
    17821 agctgcagag tttgactatt caggaacaca agcttgccaa tctttgaaag aggaagggat
    17881 tgaagttgta ttaataaata gtaatccagc tactataatg actgataaag aagtggcaga
    17941 taaaatatat atagaaccat taacaataga atttatagaa aaaataatag aaaaagaaag
    18001 accagacagt ttacttgcag gtatgggtgg acaaacaggg ctaaacctag cagttgaact
    18061 ttatgaaaaa ggtatactag acaaatatgg agtaaaaata ataggtactt cagtagaatc
    18121 tatcaaaaaa ggtgaagata gagatatatt tagagaagta atgaaagaaa taaatcagcc
    18181 tgtaatagtt agtgatatag ttacagattt acaagcaggt cttgattatg cacttacaat
    18241 tggttatcca gtagttgtaa gaccagcata cacactaggt ggaactggtg gtggtatagc
    18301 tgataatgag gaagaattaa gagaaatact ttcacatggt ctacaattaa gtcctgttgg
    18361 acaagtatta attgaaaaga gtataaaagg ttggaaagaa atagaatatg aggtaataag
    18421 agattcaaag ggaaattgta tagttgtatg taatatggaa aatatagacc cagtcggagt
    18481 tcatacagga gacagtatag ttgttgctcc aactcaaact ttaagtaaca aagaatgtgc
    18541 aatgcttaaa aaagcatctt tagatatatt aaatgcagta gaagttcaag gtggttgtaa
    18601 cgttcaattt gcacttaacc cacatagttt tgaatatgca gttatagaga taaatccaag
    18661 agttagtagg tcatcagctt tagcatcaaa agctacagga tacccaatag ctaaagtttc
    18721 gtcaaaaata gctttaggtt atactttaga tgagatagaa aatgcagtaa ctaaaaaaac
    18781 ttatgcttgt tttgaaccaa ctatagatta tgtggtggca aaaataccaa agtggccttt
    18841 tgataaattt aaaaaagcaa atagaaaatt aggaactaag atgatggcaa ctggtgaaat
    18901 tatgagtata ggaagtaact ttgaggctgc tattcttaaa gggataagat cacttgaaac
    18961 aggaaaatat tcattagttc acacaccatc tgaggagaga agtattgagg aattaaagaa
    19021 aagagttgta gtacctgatg atgagagact atttgactta gctgaaatga taagaagagg
    19081 ctataaagta gaaatgatag aacaaattac aggtatagat aaatggttta taaataaatt
    19141 taagtggata gtagagcaag aagaaaaatt aaaagttatg aaaattgaag atttaactaa
    19201 agactatctt cttgaattaa agaaaaaggg attctctgat aaaggtatat ctgacttaat
    19261 gaagataagt ccagaaaaat tatatgaatt gagaagtcta tacaatatca aaccagcata
    19321 taaaatggtt gatacttgtg caggtgagat agatgctata tctccttact attattcaac
    19381 ttatgagcaa tatgatgagg tagttgttag tgataagaaa aaggtgatag tattaggttc
    19441 tggtccaata agaataggtc aaggaataga atttgattat tgttcagttc actgtgtaaa
    19501 atctttaaga aaaatggaca tagaaactat aatcataaac aataaccctg aaactgtaag
    19561 tacagatttt gatacatcag ataagctgta ttttgaacca ttaacagaag aagaagtttt
    19621 aagtataata gaaaaagaaa agccagaagg tgtaatacta cagtttggtg gacaaacagc
    19681 tataaaattg gctaagtttt tacatgaaaa aaatatacct atactgggaa cagattttga
    19741 tgatatagat gcagcagaag atagagaaaa atttgatgat ttactagaga gactagatat
    19801 aaatagacca aaaggaaagg gtgtatggtc acttaacgaa ggtattgaag ttgctaatga
    19861 attaggttat ccagtactag ttagaccatc ttatgtactt ggtggtcaag gtatggaaat
    19921 aacttataac gaagaaaaat tatctcaata cctacaagat gcgtttgata gagaccataa
    19981 aaatccagtg cttatagaca aatatctaac tggtagagaa atagaagtag atgctatatg
    20041 tgataaagaa gatatatt
//
LOCUS       DAEORZ010000068        19644 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEORZ010000068 DAEORZ010000000
VERSION     DAEORZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.068"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            55..360
                     /locus_tag="KRH39_002164"
                     /pseudo
     CDS             55..360
                     /locus_tag="KRH39_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            373..1278
                     /gene="truB"
                     /locus_tag="KRH39_002165"
     CDS             373..1278
                     /gene="truB"
                     /locus_tag="KRH39_002165"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBG8512064.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            1409..2338
                     /locus_tag="KRH39_002166"
     CDS             1409..2338
                     /locus_tag="KRH39_002166"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBG8512065.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            2442..2699
                     /gene="rpsO"
                     /locus_tag="KRH39_002167"
     CDS             2442..2699
                     /gene="rpsO"
                     /locus_tag="KRH39_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBG8512066.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            2939..3784
                     /locus_tag="KRH39_002168"
     CDS             2939..3784
                     /locus_tag="KRH39_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG8512067.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            3909..6020
                     /gene="pnp"
                     /locus_tag="KRH39_002169"
     CDS             3909..6020
                     /gene="pnp"
                     /locus_tag="KRH39_002169"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBG8512068.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            6286..7029
                     /locus_tag="KRH39_002170"
     CDS             6286..7029
                     /locus_tag="KRH39_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG8512069.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            7187..8434
                     /locus_tag="KRH39_002171"
     CDS             7187..8434
                     /locus_tag="KRH39_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG8512070.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGI
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            8469..8714
                     /locus_tag="KRH39_002172"
     CDS             8469..8714
                     /locus_tag="KRH39_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG8512071.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            8724..9947
                     /gene="dapG"
                     /locus_tag="KRH39_002173"
     CDS             8724..9947
                     /gene="dapG"
                     /locus_tag="KRH39_002173"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG8512072.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            10060..10794
                     /locus_tag="KRH39_002174"
     CDS             10060..10794
                     /locus_tag="KRH39_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBG8512073.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            10917..13328
                     /locus_tag="KRH39_002175"
     CDS             10917..13328
                     /locus_tag="KRH39_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBG8512074.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            13331..14389
                     /gene="mnmH"
                     /locus_tag="KRH39_002176"
     CDS             13331..14389
                     /gene="mnmH"
                     /locus_tag="KRH39_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBG8512075.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQSAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            14383..15717
                     /gene="rimO"
                     /locus_tag="KRH39_002177"
     CDS             14383..15717
                     /gene="rimO"
                     /locus_tag="KRH39_002177"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBG8512076.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            15704..16246
                     /gene="pgsA"
                     /locus_tag="KRH39_002178"
     CDS             15704..16246
                     /gene="pgsA"
                     /locus_tag="KRH39_002178"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBG8512077.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLVMLFNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFKNGLILNIGGVLILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            16534..17580
                     /gene="recA"
                     /locus_tag="KRH39_002179"
     CDS             16534..17580
                     /gene="recA"
                     /locus_tag="KRH39_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBG8512078.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTNEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            17756..19297
                     /gene="rny"
                     /locus_tag="KRH39_002180"
     CDS             17756..19297
                     /gene="rny"
                     /locus_tag="KRH39_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBG8512079.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         8024 a         2001 c         3808 g         5811 t
ORIGIN      
        1 tatgcaaaat tctgtgaagc aggaaccctg tgactttagt catgggaggt tcagtcaaag
       61 gtatctagta tagttttaac tcaagacatg atgaatagaa ataatattag ctttaatgat
      121 gttgatggaa taacaacata tacaagagac ataaaaggta tagaagttgg aattttattt
      181 aaagagaaaa aacaaaatga aattaaagtg agttttaggt caaaaaatta tgttgatgta
      241 agtgaaatag ccaagttatt tggtggtggt ggtcatgtaa gggctgctgg atgtactatt
      301 agagatagta ttgataatgc aaagaaaatg gtactagagg cagtgttaaa atcaatttaa
      361 ggtgacagta aaatgaataa gattataagt atattgaaac ccacaggtat gacatctcat
      421 gatgttgtaa gtagagtgag aaagatttta aatataaaaa aagttggtca tacaggtact
      481 cttgacccag atgcttcagg tgttttacca atctgtatag ggaaagctac aaaagtatgt
      541 gaagttattt taaacaaaga taaatcgtat atatgtgagc ttactttagg tataagtaca
      601 gatacttatg atgcttcagg tgaaatttta aaaaaagttg atgattttaa atttagtaat
      661 gaagacatag aaagagcttt tgacacacaa agaggagaga taaatcaact tcctccaatt
      721 tattcagctt taaaggtaaa tggaaagaga atgtgtgatt tagtaagaag tggtagacaa
      781 tctgaaatta ctttaaaaac aagaagagta aatataaaag acataaaaat actaagtata
      841 aaaggtaata aagtaatgtt ttatgttgag tgttctaaag ggacttatgt gagaagtatt
      901 tgccatgata taggagaata tctaggttgt ggagctcata tgtcgttttt gaatagaaca
      961 tcttctggta aatttgactt agataattca ataacattag aagaattaga attattttat
     1021 gaaaataaaa ctttagacaa atacttatat gatatagatt atgtccttga ttcgtttaat
     1081 tatgttgtat taaaccctaa tgctataaaa tattatagta atggtggctc aatagatgat
     1141 aaaaggtttt taaaaaataa tttcgataaa gatgatgaat ttgtaagagt atacagtaca
     1201 gataatttct taggattagg aaaattatca aaacacaata atacaataag tgttaaaagt
     1261 gataagatgt tcatataacc aatgtattta aaaaaacaac aaaagagata gtactataat
     1321 aacttctata gtctatctct tttacttatg ctataatagt ttgtgaataa gttaagagta
     1381 aattatattt tagcggggaa catttattat ggtgattata aaatctataa aagaaatagc
     1441 aaacataaaa gaaagtgtca ttaccatagg aaattttgat ggtgtacaca aaggacatca
     1501 agttcttata ggaaaaacag ttgaacatgc aaaaaaagaa aatatacaga gtatcgtatt
     1561 tacatttgca aatcatcctg taaattactt tagaccaaac tcaacaaaaa atattataag
     1621 caatgatgat aaaataaaat tattagaaga tttgggaata gatatagttg taatcatacc
     1681 atttgatgag tatatgacta aaatacctgc aaaggatttc gtagatgaga tactagtaaa
     1741 aaaattaaaa gctaaaaaga tagtaattgg acatgacttt acatttgcaa gggcaaaaga
     1801 aggcaatgtt aatctattaa aaagcttaga acatgaattt ggttttgagg tagaagtaat
     1861 aaaacccatc aaaatcaatg atgtaagagt gagtagtact tatattagaa gtttagtatc
     1921 ccagggtgat atggctaatg tcaaggaata tttgggtaga aattacaagt tagaaggttg
     1981 tgttatacat tctaaacacc ttggaagaac cataggattc ccaacggcaa atattgacct
     2041 taagaataat atgctcgttc caaagagagg tatttatgct tctattgtac atattggaga
     2101 tgagacttat tttggtgcta caaatgtagg ctataaccca acagttaatg gaaaaacttt
     2161 gtctattgaa acaaatattt tagagtttga tagagatatt tatggggaaa atataattgt
     2221 tgaatttttg gaaagaataa gagatgaaag aaagtttaat tcaatagatg acttaaaaaa
     2281 tcaattgtat aatgatacaa attatgtgta tgaaaactat gtttgcaaaa acaagtaatg
     2341 tatgataaaa tgattaagga aaataaccat tactcggcat ttgaatactc caatcaatgc
     2401 ttagtaatag gcgtttctaa aaaaatacgg aggtaatcta aatgataaac aaatcagaaa
     2461 taataaaaga atatggaaga gctgaagggg atactggttc tccagaagta caaatagctt
     2521 tattaacagc tagaataaac gagttaaatg gacatttaaa aactcataag aaagaccacc
     2581 attcaagaag aggactatta aaaatggttg gtagaagaag aaacttatta gcttacttaa
     2641 aggaaaaaga tcttgaagga tatagagcat taatagctaa attaggatta agaaagtaat
     2701 ttcattcttt ataaaaaagc aggtatagtg tatacctgct tttttatagt taattcatta
     2761 taaagtaaaa tagttaataa taaaagtagt ttatatgtta taaaaaatta taaaatgaat
     2821 aatattgggt aagaatatta aaatagtata caaatacata atcaatttaa aaggaaagac
     2881 aggataaata tagaaatttt ataacaggat aattagttgg aataaggagg atattatttt
     2941 gaaaataaag ttagtatgcg atagtttgtg cgatattcca gatgaaatat cagaaaaaga
     3001 ttatatagag atagttccac ttacagttat ttttaatgat agggaatata tagaaggagt
     3061 agatataaag aaagaagagt tttataaaaa agtaaaagaa atcaagcaaa tacctaaaac
     3121 ttcacaagct acatatatgg agtttaaaga agtgtttgat aagttcataa cagaaggtta
     3181 tcatataatc tgtatgacag gggctgcaaa cgcatcagga acttttcaaa gtgctatgat
     3241 agctaaaaat gatgtaaatg aaaatgaaaa aatacatata tttgatacta gaaatttatc
     3301 tctaggtagt ggacaatatg ttataaaggc atgtgaactt cttgaagaag gactgggatt
     3361 tgaagaaatt atagatgaat tagaaaatac tcgcagtagt gtaagattgc tttttgcacc
     3421 atatacactt gattttttaa agcagagtgg aagagttcca gttgcaactg ctttgatagg
     3481 aaatatgtta aatataaagc ctatattttt ctttgataat ggggaagcta agttggtaaa
     3541 taaggttaga ggaataaaga atatagcaag taaattggtc gatataattt tagaaatgaa
     3601 tgaagggtct ttagaaggaa aaatcgtcac aataggttgt ggagataatt tacacgattg
     3661 tgaaatatta aaagatgaag taaataaaag aataaaagca agaagagtat tatttactcg
     3721 aggaggagtt tctatttgct ctcatacagg gccagatata ttggctataa gttgttctaa
     3781 ttagtagtaa tataaggttt ttgcatatat atgtgaagat aattttatat tgttaattta
     3841 tagtattttt gataatatag taaatagagt gatatacgtg ttttttaata agaggaggac
     3901 aagcattaat gtttgaacat aaaatattta aaatggattt tgcaggaaga gaactttctg
     3961 tagaaatagg aaagatttgt gaaatggcta gtggtagttg tatagttcga tacagtgata
     4021 gtatggtaat ggtaaataca actaaatcag ccaaacctag agacggaata gacttttttc
     4081 ctttaagtgt ggattatgaa gaaaaattat actctgtagg taagatacct ggaggttttt
     4141 taaagagaga aggcaagcct tcagagaaag ctatacttac atcaagattg atagatagac
     4201 caataagacc tttattccca aaaggattta gaaatgatgt tcaggtagtt gcaacagttc
     4261 tatcagtaga ccaagattgt acaccagata tagtagcaat gataggttct tcaatagccc
     4321 tttctatatc agatatacca tttaatggac caacaggttc agtatgtgtt ggccttgtag
     4381 atggtgcatt tgttgtaaat ccaaacgcag agcaaagaga aaaaagttca atgcatctag
     4441 ttgtttcagg tacaaaggaa gcaataatga tggttgaagc cggagctgat gaagttccag
     4501 acgaggtaat gcttgatgct attttatttg ctcaccaaga aataaaaaaa atagttgagt
     4561 ttatagaagg tatagttgca gaagttggaa aagaaaaaat gcctgttgaa ttataccatg
     4621 caggagaaga aataactcaa ttggttcgcg aatttgcaac tgataagatg aaaaaagcag
     4681 ttcagacttt tgaaaagtta gagagaatgg aaaatatgga tagagttaaa gaagaaactt
     4741 tagctcactt tgaagaaact ttagaagatt ttgaagattt tgttggagat atagaagaag
     4801 tattacaaga tataataaaa gaagaagtaa gaaaacttat agtacatgaa aatgtaagac
     4861 cagataatag aaaacttgaa gaaataagac ctatatggtg tgaaactggc atgataccta
     4921 gagctcatgg ttcagccata tttactagag gacaaactca agtacttaat gttgctactt
     4981 taggtgcttt aggagatgtt caaaaactag atgggttaga tgaagaagaa aacaagagat
     5041 atatgcatca ttataatttc cctgcatata gtgttggaga agctagacca tcaagaggac
     5101 caggaagaag agaaatagga catggtgctt tagcagaaag agctttatta ccagtaatac
     5161 catctcaaga agaatttcct tatgctatca gattagtttc tgaagtatta agttcaaatg
     5221 gttctacatc tcaagcaagt gtatgtggct caacattatc tttacttgat gcaggtgtac
     5281 ctataaaaga tatggttgca ggtatagcaa tgggacttat aaagcatgat ggtaaggttg
     5341 cagtgctttc agatatacaa ggtatggaag accatttagg cgatatggac tttaaagtag
     5401 ctggaacaga gtatggtata acagcaatac aaatggatat aaaaattgat ggtatagata
     5461 aagaaattct acaacgtgct ttaaaacaag ctaaagaggg tagaatccat atactaggag
     5521 agatgagaaa aactatatct cagccaaaac cagaactttc accatatgca cctaagattg
     5581 ttaaaatgca aataaatcct gacaagataa aagatgtaat aggacctggt ggaaagataa
     5641 taactaagat aatagacgaa actggagtta agatagatat tgaacaaact ggggaagtat
     5701 ttatatcagg tattgagatt gatatgatta agaaagctca agaacttata aataatatag
     5761 tagttgaacc agaagtagga aaaacttata aaggtaaggt aagtagaata atgaactttg
     5821 gagcatttgt tgagatactt ccaggaaaag aaggattact tcatatatct catatagctc
     5881 atgaaagagt tgccaaagtt gaagatgtat tgaatatagg ggatgaagtt gaagttaaag
     5941 ttacagagat tgatgaaaaa ggaagagtta atttatcaag aaaagttctt ttaccaaaac
     6001 cagaacataa aaataaataa cttattatat aattaaaagc aaagtcttag gattttgctt
     6061 ttttaaatac aaaaaagtag ataattttaa aatgatagtt atggatggct tatagttctt
     6121 tttctgtcag gctttactct tataaagaag ctatttaaaa gctgattaat agagagcttt
     6181 attgatatat atagaatcac ttaagttata tttagtgcat aaacagagct tatttttaat
     6241 agtattaaat aagaaacaat ttgtacataa ttagggggag ttttaatgat aatgatggta
     6301 cttaataaga gtaagctcaa aaaaatcata atactaattt tagtagtgtt attagtatta
     6361 tcaggagggg taatggtatt aaagttattt aacaacaagc cagtatttaa tttatttaat
     6421 ggtgtagatt taccatatga aagaggtaca aaggataaaa gtggatatgt tgcattaacc
     6481 tgtaatatag accttggatg ggaaacagag tacgtagaat ccattttaga aacacttaaa
     6541 aaagaaaatg taaagattac atttaatgta actggaaaat gggcagaaaa gaataaggat
     6601 gaattattaa agataaaaaa acaaggacat gaaataggta atcatggata caaacattta
     6661 gattattcta cattatcata tgaagacaat tatgagcaaa tagagacctc taaaaagata
     6721 attgaagaaa ttataggtga aaaaactaaa ttttttcaag caccagcagg ttcttttggt
     6781 ccagaaacag taaaggctgc taaagcatta gggtatactt ctatcaaatg ggatgccgat
     6841 actatagatt ggaaatacaa agaccaacca gaggtaatca ttgatagaat gaagaaaaaa
     6901 gacattaaag atagtagtat aattttaatg catccaacca acgcaacgac aaaatgtata
     6961 gatgatatta tagctattgt aagagaaaaa ggactaaaac caggaaaact tagtgatgta
     7021 tttaagtaga caaagtataa atatttggtt aagattgtat aagttgtagt tgaattaatt
     7081 aaaagactga cttataatgt aatacatata aaaaataaat ataattaaaa catagtgttt
     7141 tcaataatcc aatatgaaac aaagaataaa ttgttaggag aatgtaatgt ataagacaaa
     7201 aatactggaa aatgggctta caataattgg tgaagaaata ccatatctaa agtcaataac
     7261 attaggtatt tggataaatg caggctctag gatagaagaa gcacaggtga gtggaatttc
     7321 tcattttata gagcatatga tgtttaaagg aaccaaaaac agaacatcaa aagagattgc
     7381 tagttcaata gataatttgg gtggtcaaat aaatgcattt acaagtaaag aatgtacatg
     7441 ttattatgta aaactaatag atgagcatat agatacagga attgatgtat tgagtgatat
     7501 gatactaaat tctaaatttg ataaaaatga tatagataag gaaagactta taatattgga
     7561 agaacttaag atgtatgaag actctccaga tgacttatct tatgatttat tagtggaaaa
     7621 tatatatgct aatgatggat taggaatgaa tataattggg acaaaagaat ctctatacaa
     7681 tataactaga gaatccatgt tagaatactt aaataagtat tatattccaa ataacgctgt
     7741 tatttctata gctggaaact ttaactttga tgatatggtg gagaaaatca aatcaaaatt
     7801 tggacactgg gaaaagaaaa atttaagtat agacataagt gaggctaagt ttaatccttg
     7861 ctttatatca aaaaataaag atactgaaca ggtgaactta gctatgtgcc taaaaggtat
     7921 tccttttgaa aatgatgaag aagtctattc aatggcagtg gtaaataata ttttcggtgg
     7981 aagtataagc tcaaggcttt ttcagaaaat aagggaagaa aaaggtttag tatattcaat
     8041 atactcatca cagacattat ataggaaatg tggtgaatta ggtatatttg caagcatgag
     8101 tacagaaaat ttacaagatg tatataattt aataaagaaa gaaattgaaa atataagaga
     8161 aaactatctt acagaaaaag aaatatctga aagcaaggaa cagttaaaag gaaattatat
     8221 attggattta gaaagtacta gcagtagaat gatgtctact ggtaaatcta tgttacttag
     8281 taaaaaagta aaaactacag atgaaatact tgaatgtata aataatgtta atattaattc
     8341 aataaagaag gttgttgata aggtattcaa tatcgaaaat attggtacat gtatagttgg
     8401 aagagatgta gaaaagattc tacacttgga ttaatattga aaaaagtaaa tctctggagg
     8461 tataaaatat gaatttatct gaaatagcag gtaaggaaat agttaacttg gtcacaggag
     8521 aaagattggg ggtcattgga gaatgtgacc ttatactaga tgaaactaca ggcggtatat
     8581 tagcgcttct aattccaaaa gaaaaaggat tttttgccag aagaaaagat aagtcatttt
     8641 tagaagtacc atggagaaat gtaaaaaaaa taggtaatga catgataatt atagaatatg
     8701 agggatatat ttaggaggaa aaaatgagta ttttagtgca aaagtttggt gggacatcag
     8761 tagaatcata tgaaaaaatg aatgaagtat gtaagatagt aaaagcatac aaaaaaaatg
     8821 atgaagaatt acaattagta ttagtagttt cagcaatggg aagaaaagga gctccatatg
     8881 ctacagatac acttataaat ttgtgtagtg cagtaaatga tgagccaagt aaaagagaac
     8941 ttgatttaat aatgtcttgt ggagaaataa tatcaggaac aattcttgct aatttattga
     9001 atgcacaggg gatagattct gttttcttta ctggtcagca agcaggtata attacaagtg
     9061 atgaatattc taatgcaaag ataaagtata taaatcctaa aaaggtaaaa agagctttag
     9121 atgatggtaa ggttgtagtt atagctgggt tccagggtgt aacagatgat ggagaaataa
     9181 ctactcttgg aagaggcgga agcgatacat cagcagttgc tattggtaaa gctttagaat
     9241 gtgaaacagt tgaaatttac actgatgtgg atggtataat gactgcagac cctagagtag
     9301 aaccaaacgc taaagtattg agttttatag actatgagga agtattccaa atggctgaca
     9361 aaggtgcaaa agtaatacat ccaagagcag tacagttggc taaaagtgga aatataactt
     9421 tagccataaa gaatacaatg aatccaactt ttgaaggtac aaaaataggt tctttatgta
     9481 gacacttaga agacaatata gaatatgaac aagaaagaga ttttaaggtt gctgttgcaa
     9541 ataaagatag cgtagcacaa gttaagatta agtcagcaga agaagtgttt acagaagtat
     9601 taaatgagat agaaaagaaa cttataacta tagatatgat aaatttcttt atatcagaaa
     9661 aagcatttgt tgtagaagat gctgatataa aatcattaaa ggaaatacta gataaatttg
     9721 aattagatta tgaagtaaat agagactgtg ctaaagtaac attgatatgt tcaagaattg
     9781 ctgatgaaat gtctggtatc atgtcaaagg tagttagagg attatcaaaa gcaggggtat
     9841 ctcttttaca aacatcagac tcaaatatga ctatatcttg tttagttagt gaagaagata
     9901 tgcatacagc agtacatgct atacaccaac aattttattt aaaataaact gtataataca
     9961 attatttttt aatcaaattt aagaatttca tatatatctt aatatatttg atttatttgg
    10021 taaaactaaa ttaaatataa ttttgatagg agttttttta tgaaagatta tagagaagat
    10081 aatgaaagat ttgataaaga taattgtagt gaagaatcta gttgcgaaga gagagaatgt
    10141 ataaagcaat ttggtaccaa tgaaatgcca cctcaacctc ctaaagatat tcaatgtata
    10201 actataattg gagaaattga gggacatttt ataggaaatc ctcaaaaaaa agctacaaag
    10261 tacgagcata taattccaat gctatactca attgaggaaa gcaatgatgt taaaggagtg
    10321 ttagttgttt taaacactgt tggaggagat attgaagcag gtttagctat tgcagaactt
    10381 ttaaacagta catctaaaaa agtagttact cttgttctag gaggaagtca tagtataggt
    10441 gtccctcttg ctacagcagg agattactca tttatagcac caacagcaac catgattata
    10501 catccagtgc gaactactgg tcttgtgatt gggataaatg agacttttga gtattttaaa
    10561 aagatgcaag atagaataat tcaatttata ataagaacat caaatatcaa aaaagatgta
    10621 cttgaaaaac ttatgcatga aaaagatgaa cttgtaagtg atgttggtag tgtattaata
    10681 ggaaaagaag ctgtggatta tggtcttata gatgaagttg gaggactaaa agaagcactt
    10741 aaaaaattaa gagagcttat aaaagaatct gaggaagaaa aaaataatga ttaaaatttg
    10801 tttaaataat aaaatttatg atataatgat gtaataaagt aggagtgtta gtgttaaggc
    10861 attagcactt tttgctgtga aggggtaaaa cttgctaaaa ataaggagtg atattaatgg
    10921 ccacgaaaaa gaagaaaaaa aagaaggtaa ataccttaag ttttaatgca gaatatcata
    10981 atttaataac aatatttata ggtgtatttt tattatatag tttaaattct aattctatgg
    11041 gatggatacc tgtgttaatg caaaatcttt ttaagggact atttggaggg ctttcaatag
    11101 caataccatt tatagtaatt ataacaggac ttttaggatt ttttgatgga aatgaatata
    11161 tatataggct aagaaagact aaactatact acataattat tttatttata tttgtatttt
    11221 atggattatt aaatgcagga actcttcctg tagatagccc actaaaaggt aacatgtttg
    11281 atgatgttat gaaattgggt gtaagtggac aaggtagtgg tttgatagca acaactattg
    11341 cgtattatat gagtaaaata tttggtatag caggtggatg gttaataagt atcttcgcac
    11401 ttatactttc tgtaatgttt atatttaata tttctataaa agatttggtg tcaaatgcaa
    11461 aatcaaaatc taaagcatct aaagacagca atttaacgtt taaagataaa attgcaaata
    11521 tgaaaaaatc tgcaatagat atgatgactg atgaagtaga tgatactact ataaataaaa
    11581 aaccaggttt ctttaaaggg ttaatgtcaa aaggtcttaa taatgatgat gaagaagatg
    11641 aatattttga agaagaaaat agtgatggtg tagatgataa aactataaaa atagttggat
    11701 ttaataaggc agaagatgag tatttagaaa tattagaagg aactcaaagt atgccagagt
    11761 tagatgtttt aaaagaatta caaaaagcta ccaatgaaaa tccagttgta gatactaaac
    11821 cagaaaaaaa agttgatata gctaaaccta atttaaatat agaaaaaact caacctatga
    11881 gtattgtagc agagccagtt aatgaggatt attcaaacta taaaaagcct agcattgagc
    11941 ttttaaataa agtaaataag aagtctgatg aaaatggtaa aaagaaagtg ttgaaaaatg
    12001 cttctctttt agaaaagaca ctatcagatt ttggagtgga agcaaaaatt aatcaagtta
    12061 cagtcggacc tactataaca agatatgaaa tacaaccaag tccaggagtc aaggtaagta
    12121 aaattgtaaa tttaactgat gatatagctt taagtttagc agctaaaagt ataagaattg
    12181 aagccccaat tcctggtaaa agtgcaattg gaatagaagt tccaaatgaa gaagctcaaa
    12241 tggttggtgt gagagaagtt ctagaaagtg aagaatttaa taattttaac tctcctcttg
    12301 caatgggact tggaaaagac gtagcaggaa aaattataat tggagatata ggtaagatgc
    12361 cacacttact aatagctggt tctacaggtt caggaaagag tgtttgtgta aatacattaa
    12421 taagtagtat tttatataag gcaaatcctg atgaagtaaa attactatta atagacccaa
    12481 aagtagttga acttgcaaat tataatggaa taccacatct attgatacca gtagttactg
    12541 accctaaaaa agctgcaaat gctttaaatt gggcagttac agagatgaat agaaggtaca
    12601 aattatttgc agatgctcaa gtaaaagatg taacaagtta taatgagaaa gcggaagaaa
    12661 aacttcctaa aatagtaata ataatagatg agttagcaga tttaatgatg gcaagtgcaa
    12721 atgatgtaga agattatatt tgtagattag ctcaaatggc aagagctgct ggaatgcact
    12781 tgattgtagc aactcaaaga ccatctgttg acgttataac aggggtaata aaggctaata
    12841 taccatctag aatagcattt gcagtatcat ctcaaactga ttcaagaaca atccttgata
    12901 tgggtggtgc agagaagctt ctaggtaaag gtgatatgtt attctatcca cttggtgcag
    12961 caaaaccagt tagacttcaa ggggcattca tatcagagag tgagtcggaa aaagtaatcg
    13021 attttgtaaa aagtcaggtt aaagatggaa taaaatatga agaagatata attgaaacta
    13081 tatctaaggt taatacttca aagggtagtg atgaggatga gtttttgagt gaagcgatag
    13141 aatttgtcgt ggaaagtggt caagcatctg cctctatgct acagagaaga tttaaaatag
    13201 gattcaatag agcagctaga cttatagatt ctatggaaga aagaggaata attggagcaa
    13261 gtgagggaag taaaccgaga aaggtattaa taagcaaaca ggatttgcag aatttagaag
    13321 gtgaatagaa ttgtcagtta taaaaataga aaaagcactt aagctagata aagtaatatt
    13381 tgtagatgtg agaactgaag gagaatatga agaagaccat atattgaatg caatcaatat
    13441 gcctcttttt aaaaataatg aacataatga agttggaaca atttataaga tgcagggaaa
    13501 acatgaagca atacaaaaag ggtttgacta tgtatcgtat aaattaaagg atatatattt
    13561 acaatctgca gaacttgcat taaattatga taatattgtc atatattgtg caagaggcgg
    13621 aatgagaagt ggaagtatag taaatttatt atcatcatta ggtgtaaatg tatatcaact
    13681 tgaaggtgga tataaggctt atagaaattt tgttttagag tatttaaggc atgtaatgga
    13741 tacaaaaaac tttgtatctg ttcatggatt gactggtaca ggaaagactg atttgttaca
    13801 tttattagat gaaaaaggaa tagacgtact ggatttagaa ggcatagcta aaaacagtgg
    13861 ttctaccttt ggttttataa cttttgataa aaagccacca tctcaaaaga actttgaaac
    13921 aaaactattt gaaagtatat ttttttcgaa agagaattat atatttgtcg agagtgaaag
    13981 caaacgagtt ggaagtgtta cagttccaaa tgaaattttc gaggcaatga cacgagaagg
    14041 acatcatata ttattagaat gtgatattga aaatagagtc gatagacttt gtagagatta
    14101 tatttatgat aaagatgaag gtaatatatt gattttaaaa gaatgtataa acaagtttag
    14161 aaaaagattg ggtaataaag tagtagatga ttatattgat ttattagaat caggtgaata
    14221 taaagaactt gtaaaaagat atcttttaga atactacgac ccactttata tgcattcagt
    14281 agagaaatat aagtatagta agattataaa ttttaatgat acacaaaaag ctttagaaga
    14341 tgttatagac ttatatgaga gtatattgga aggagaaagt gaatgttaaa aatagcattg
    14401 gaatcattag gatgctcaaa aaatttagta gatgcagaaa taatgatggg aattttaaat
    14461 aacaaaggct ataaattaat tggagatttt gaagaagctg atgtaattat agtaaataca
    14521 tgtgggttta tagagtcagc taaacaagaa tctatagata cgataataaa ctttgcagag
    14581 ttaaaaaaaa ctggcaattt aaagttgttg atagtaacag ggtgtctagc ccaaagatat
    14641 tctgaagaat taaaaactga aattcctgaa atagatgcta tagtaggaac aggaagctat
    14701 caaaatatag ataaaatact taaagaatta agtgaaatgc atcaaattgt aagccttaat
    14761 gatatagagt ttgtatttaa tgaagattta ccaagatata tatctacacc tagctatatg
    14821 gcctatttaa aaattggaga aggatgctcc aataattgta cttattgtat aataccaaaa
    14881 ctaagaggta aatatagaag tagaaaattt gaagatataa ttaaagaagc taagaaatta
    14941 gctgaatctg gagtaaaaga acttgtggtt atagcacagg atacgacaaa gtatggtttt
    15001 gatttatatg gaaaagaaag attatcagaa cttttagaag aacttgcaaa aatagatgga
    15061 tttaagtgga taagggttat gtattcatat ccagaatcta taacagaaga attgatacaa
    15121 gttataaaga aatacgataa tatatgtagt tactttgata tgccaataca gcatgcaagt
    15181 aacaatattt taaaattaat gaatagaaaa actactaaag aagatatatt aaataaaatc
    15241 aatctgataa gaagtaatat accagatgct atacttagaa ctacgataat agtagggttt
    15301 cctggtgaaa cagaggatga cttcaaacaa cttgtggatt ttgttgaaga ggttaaattt
    15361 gatagacttg gtgcatttgc atattcaaga gaagaagata caccagcaga tagattgcca
    15421 aaccatatag atgaagaagt aaaaatacaa agaagagata ctttaatgat gattcaacaa
    15481 aaaatatctg aagaacttaa tgacaaaaag ataggaaaaa cttatgaggt gttaatagaa
    15541 gaacagatag aagataatgt atatacagga agaacacaag gtgatgcgga agaaattgac
    15601 agtatagtgt atgtgaaaag tgtagacaat cttgaagtag gagaatttgt aagtgttcaa
    15661 ataaatgatg caatggaata cgatttaatg ggagatgttt tatatgaact tgcctaacaa
    15721 gttaactttg tttagaatat ttttaatacc agtatttgtt ttagttatgt tgttcaatgt
    15781 accaaataaa tttttaatag cttgtattat ttttataata gcttcaatta ctgatgcttt
    15841 agatggaaaa atagctagaa aatacaatct agtcactgac tttggaaagt ttatggaccc
    15901 attagcagat aagctattgg ttatttcagc acttacatgt atgatagaag accatcttgt
    15961 aagtagttgg atggtaatta taatagtggc tagagagctt acagttagca tattaagagc
    16021 tatagctgct gctgatggaa aagtaatcgc agctggaaac agtggaaaac ttaaaacgat
    16081 aactcaaatg gtatcgatag tatttttatt attaggtgct cagtttaaaa atggattaat
    16141 attaaatata ggtggagttc ttattcttat agctacgcta cttacattgt attctggatg
    16201 ggaatatcta tataaaaata aagaactttt tatgtcttct aagtaaaaaa ttatgcttta
    16261 ataaataaaa actaatgatt aataaataaa aaataacaac ttaaaatttt aaaaatgtaa
    16321 aaaagggtat actattatag tatacctctt ttcattttaa gaatattttc ttttgtgtat
    16381 ataattatta ataatggttt ataaataaaa tgtaatgaat aaaaatataa tcattagtag
    16441 cgtttttaaa ttgactaggt ttgatttatg ttatataatt aatatataac aagagaacaa
    16501 atgtttgtag ataaatgaag ggatggtata gagatgagtg tagatcaaga aaaattaaaa
    16561 gcgttgaatg aagctttagg taaaattgaa aaagattttg gtaaaggttc agtaatgaaa
    16621 ttgggagaag caacatctat gtctatagat gttatatcaa caggagcgat tggtttagac
    16681 atagctattg gtataggagg tctacctaga gggagaatag ttgaagtata tggtccagaa
    16741 tcttctggta agactactgt tgcgcttagt tgtgtagcat cagctcaaaa agatggagga
    16801 atagctgcat ttatagatgc agaacatgca cttgacccag tatatgcaaa agctttgggt
    16861 gtggatgttg acaacctaat aatatctcaa ccagatacag gtgaacaggc tttagagata
    16921 gcagaggcat tgataagaag tggagcgata gatataatag taatagactc agtagcagca
    16981 ttagttccaa aggctgaaat agatggagat atgggtgatt ctcatgtagg tttacaagct
    17041 agacttatgt cacaagcact tagaaagtta actggttcaa ttaaaaaatc aaattgtgtt
    17101 gctatattta taaaccagtt aagagagaaa gtaggaataa tgtttggaaa cccagaaact
    17161 actactggag gacgtgcact aaaattctat tcatcagtta gattggatgt tagaaaaata
    17221 gacacaataa aacaaggtga taaagttata ggtagtagaa ctagagttaa agttgttaaa
    17281 aacaaagtag caccaccatt taagcaagct gaatttgata taatgtatgg agaagggatt
    17341 tcaaaaattg gagacctttt agatatagct gctgatgtag atatagttaa aaaatcaggt
    17401 tcatggtata gttacaatga tactaaactt ggacaaggaa gagaaaatgt taaaaaattc
    17461 ttggaggata atttagattt aacgaatgaa atagatgaga aagttagagc attttacaat
    17521 ttaaatgaag aacatgaaga atcaggtact tcagtatcaa aagaaattgt agaagaataa
    17581 gtaaaaatac aaaaaaataa ctagtaatta aagacatgcc acgctcccta ttagcttgac
    17641 acaatgttga aaaaattata aaattaagtg aggataaaat atctaaattt aattttaatt
    17701 attaggaata ttttttaatc atagtgatga cattttggga ttattaacga aggaggtgga
    17761 tgtcatagat agtgtagtaa tgattgtaat aggtgcagct gttggtatta tagctggata
    17821 ttttgttaga aaaaatatat ctgaagcaaa aattggacaa gctgaaaact tggccaaaga
    17881 aataatagat aaagcacacc acgactcaga aacagtacaa aaagaaaagt tactagaagc
    17941 taaagaagag atccataagt ggagaacaga agctgaaaga gaaaacagag aaagaagaac
    18001 agaagtacaa aagtatgaaa aaagagtagt acaaaaagaa gaagtcttag atagaaaatt
    18061 acaaaattta gaatctaaag aagtaaacct aagtgaaaaa ttaaaggtag tggaaaagaa
    18121 agaagaagaa gttgaagtaa taaaaactca acagttagaa aaattagaaa gtatatcagg
    18181 aattacttct gataaggcca aagaaatcat cttaaccaat gcagagagag atgtaagacg
    18241 tgaaatgtct ataatgataa aagagataga aagtcaagct aaagaagaag ctgataagaa
    18301 atcaagagaa attataggat atgcaattca aaaatgtgca gcagatcacg tagccgaaac
    18361 tactgtaaca gtagtaagct tgcctaatga tgaaatgaaa ggtagaataa taggtaggga
    18421 aggtagaaat ataaggactt tagaaacctt aacaggtata gaccttataa tagatgacac
    18481 tccagaagcc gtaattttat caggatttga tccaataaga agagaaattg caagaattgc
    18541 tctagaaaaa ttaatagcag atggaagaat acatccagct agaattgaag aaatggttga
    18601 aaaggcgaga aaagaagttg acaatattat aaaagaatat ggtgaacaag ctgcatttga
    18661 aactagtgta catgggattc atccggaatt aataagatta ttaggtagac ttaactatag
    18721 aactagttat ggacaaaatg tacttaaaca ttctatagaa gttgctcata tagctggtat
    18781 aatggctgct gaaataggag cagatattag attagctaag agggcaggtt tattacatga
    18841 tataggtaaa gctgtagacc atgaaatgga aggtacacat gttgaaatag gtatggattt
    18901 acttagacgt tataaagagt ctaaagaagt tatacatgcc atgagtactc atcatggaga
    18961 ttacgaacct caaacagtag aggctgtatt agttacagca gcagatgcaa tatcagctgc
    19021 tagacctggt gcgagaagag aaactttaga agcatatata aagaggcttg aaaaattaga
    19081 agaaatagct aattcatatg aaggtgtaga aaaatcattt gctatacaag ctggtagaga
    19141 aataagaata atggtaaaac cagaagctat aaatgatgaa gaaatacatc tattggctag
    19201 agatatgact aagaaaatag aggatgaact tgaatatcct ggacaaataa aagtaagcat
    19261 aataagagaa acaagagcta tagaatatgc gaagtaaaag aaataatatc taaataataa
    19321 gaaaatattt ttagaaattt aaatttgcca caagatatga taatatgtct tgtggctttt
    19381 aaattgttta agtttaattt tatataatga aaaaaaatac acttttacat acaagtatta
    19441 tggtttgcat tattattact cttggttttg tggctatctc tacaattgga tatcaatcta
    19501 ataccaatat ttttaaaatc aataccttac tacaaaataa atagtaaata ctacagtaaa
    19561 atctaaaaat aggatataat agtattaata taagaagatt ataaataaaa cctgctaatg
    19621 aaaatagatt gtttttagca ggtt
//
LOCUS       DAEORZ010000069        19383 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEORZ010000069 DAEORZ010000000
VERSION     DAEORZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.069"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(53..1246)
                     /locus_tag="KRH39_002181"
     CDS             complement(53..1246)
                     /locus_tag="KRH39_002181"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBG8512080.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINVENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(1269..1934)
                     /locus_tag="KRH39_002182"
     CDS             complement(1269..1934)
                     /locus_tag="KRH39_002182"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBG8512081.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKSNTILLTKNVGDFVNLESDVIGKYVNNFMVNNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(1968..3080)
                     /gene="ribD"
                     /locus_tag="KRH39_002183"
     CDS             complement(1968..3080)
                     /gene="ribD"
                     /locus_tag="KRH39_002183"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBG8512082.1"
                     /translation="MSFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCIIVKDSNV
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEEEARDLNEAFFYYIKNKKPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESKKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(3212..3333)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3522..6005
                     /gene="recQ"
                     /locus_tag="KRH39_002184"
     CDS             3522..6005
                     /gene="recQ"
                     /locus_tag="KRH39_002184"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBG8512083.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFLELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFINTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDKLYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDLKNINSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMVLANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      6094..6205
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6347..7648
                     /gene="thiC"
                     /locus_tag="KRH39_002185"
     CDS             6347..7648
                     /gene="thiC"
                     /locus_tag="KRH39_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBG8512084.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            7702..7896
                     /gene="thiS"
                     /locus_tag="KRH39_002186"
     CDS             7702..7896
                     /gene="thiS"
                     /locus_tag="KRH39_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBG8512085.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            7889..8692
                     /gene="thiF"
                     /locus_tag="KRH39_002187"
     CDS             7889..8692
                     /gene="thiF"
                     /locus_tag="KRH39_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBG8512086.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            8755..9525
                     /locus_tag="KRH39_002188"
     CDS             8755..9525
                     /locus_tag="KRH39_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBG8512087.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            9542..10645
                     /gene="thiH"
                     /locus_tag="KRH39_002189"
     CDS             9542..10645
                     /gene="thiH"
                     /locus_tag="KRH39_002189"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBG8512088.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            10646..11335
                     /locus_tag="KRH39_002190"
     CDS             10646..11335
                     /locus_tag="KRH39_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG8512089.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMKSKDIK
                     KTISNYNEKLN"
     gene            11523..12917
                     /gene="yfcC"
                     /locus_tag="KRH39_002191"
     CDS             11523..12917
                     /gene="yfcC"
                     /locus_tag="KRH39_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBG8512090.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            13434..14801
                     /locus_tag="KRH39_002192"
     CDS             13434..14801
                     /locus_tag="KRH39_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBG8512091.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            14826..15434
                     /locus_tag="KRH39_002193"
     CDS             14826..15434
                     /locus_tag="KRH39_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBG8512092.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            15482..15853
                     /locus_tag="KRH39_002194"
     CDS             15482..15853
                     /locus_tag="KRH39_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBG8512093.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            16032..16370
                     /locus_tag="KRH39_002195"
     CDS             16032..16370
                     /locus_tag="KRH39_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBG8512094.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            16836..17339
                     /locus_tag="KRH39_002196"
     CDS             16836..17339
                     /locus_tag="KRH39_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBG8512095.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            17386..18345
                     /gene="moaA"
                     /locus_tag="KRH39_002197"
     CDS             17386..18345
                     /gene="moaA"
                     /locus_tag="KRH39_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBG8512096.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            18349..18819
                     /gene="moaC"
                     /locus_tag="KRH39_002198"
     CDS             18349..18819
                     /gene="moaC"
                     /locus_tag="KRH39_002198"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBG8512097.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            18855..19286
                     /locus_tag="KRH39_002199"
     CDS             18855..19286
                     /locus_tag="KRH39_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBG8512098.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         7583 a         2084 c         3338 g         6378 t
ORIGIN      
        1 taattttaat tataaaaaac tacttagtag aaaaatatca tcatataatc acttatataa
       61 tatctaaaag atgtcccatt ttatcttttt tagtttttaa atatagctta ttaaagtcat
      121 taatttcttc attaataggg attctatttt cgatttttat tccatatttc tctaactgat
      181 ttattttatc agggttatta cttaaaagat ttatactttt tatgtttaaa ttttttaata
      241 tttgagcagc catatagaaa tctctcatat cttcttcaaa tccaagcatt aaatttgctt
      301 caacagtatc ataaccttct tcttgaagtt tataagcctt aattttattt aaaagaccaa
      361 tacctctacc ttcttgcttc atataaatta aaactccact tccattttcc acaattttct
      421 tcatagaaga ttctagctgt aatccacaat cacattttag agagtggaac acatctcctg
      481 ttaaacattc agaatgaagt ctaactagag tattttctac atttatattt ccataagtaa
      541 gggcaatgtg ttcttcattt gaataagtat ctctatatcc tatgatttca aagtttccat
      601 attttgttgg aaggaaagca ctagatactt tttctatagt agtttcattt atttttcggt
      661 attctataag gtcttttata gtgattattt ttaaattatg cttttctttg aaaatcatta
      721 aatcatcaac tcttgccatt gtaccatcat ctttcattat ttcacagata agaccaatct
      781 ctttaaaacc tgcaagtcta gcaaggtcta ctgaagcttc tgtatgtcca tccctgacaa
      841 gaactccacc agtttttgct atcaaaggaa agatgtgacc aggtgttttg aaatctttac
      901 ttgtagaatt ttcatcacag aacaatttta cagtttcagc tctttcatat gcagatatac
      961 cagttagagt atctgcccca tctatagtaa cagtaaaggc tgtttgaaat gtatctgtat
     1021 tttctctaac cataagaggt atattaagac ttttaaattt tttttcagtg gcaggtagac
     1081 aaattagacc tctaccatat gtagccataa aatttattga ttcaggagta gccatttcag
     1141 ctgccatcaa taaatctcct tcattctctc tactttcatc atcaaccacg attaccattt
     1201 ttccattttt tatatcttca atagcttctt caatagtatt aaacatgtaa ttcactcctt
     1261 tattattttt aaaacccatt tttaaataaa aaatctttat ctatatttga tttatggtta
     1321 ctactattag aatttaattc cttgtagtta ttaaccataa aattattaac atactttcca
     1381 attacatcac tttctaaatt aacgaaatct ccaacattct ttgtcaagag aatagtgtta
     1441 cttttagtat gaggtataat agaaacctta aatatttcat catctaaata tgatatagtt
     1501 aaacttacac catcaatagc aacagaacct ttgagtatca ttgatgataa aagtttttta
     1561 tcaggtcgta tgcttactaa aactgcattt tcatcttttt caaatttaca gatagttcct
     1621 ttaccatcaa catgacctgt tactatatga ccaccaaatc ttgaagaaag actcatagct
     1681 ctttcaagat ttactttgtc atttgcattt aataaaccta aatttgtgga acgaattgtc
     1741 tccatcataa catcggctgt aaattcattt tttccgaggt ttgaaacagt tagacaaact
     1801 ccatttacgg ctatactatc tccaagattt gtgccatcta atattttatt tgatagtatg
     1861 gttacttttc cactcttacc attagcagta atttttctaa gtattccaac ttcttctact
     1921 atgccagtaa acatcctaac ctcctatatg catgtattaa caaaaattta atttaaaaca
     1981 tctgattcta taagaatatc cccatctatc aaagttacat tatttattgt taactgtgtt
     2041 gaatctgaca aatgatttat tccttctcca gaaacgaatg taggtgcatc tttaccacct
     2101 ataatcttgg gtgatagaaa taatttgact ttatcaacta acttatttct aaagagactg
     2161 tcattgagag tagcaccacc ttcaactaga acagaatcaa tgtttaactc tcctaattta
     2221 tacattacaa attccaaatc tactaagtta ttttttgaag gagaaattat tgtttcaaca
     2281 ttattttctt ttaacataga taatttttta ctttccttgc aacatgtgaa aactatagtt
     2341 ttactggttt tatctttaac caaattacta ttaagtggca actttaggtg tgtatctatt
     2401 actattcttg tgggatgaga cactttttct tgattgagcc tacaagttag taaaggatta
     2461 tcgttcaaaa ctgtatttat gccaaccatt atactttggt atttatttct gtatttatga
     2521 gttagatacc ttgatgattc attagatatc cattttgatt ctagtgattt tgtagcaatt
     2581 ttaccatcta gacttactgc tgattttaca atacaaagag gttttttgtt ttttatataa
     2641 taaaaaaatg cctcatttaa atctcttgct tcctcttcta atattcccac agtaacatca
     2701 atattgttat cttttaattt ttttactcca ttaccacaaa caagtggatt tgggtctaat
     2761 gtagatatta caactctctt aatcttattt tgtataattt tatctacaca aggaggcgtt
     2821 tttccataat gagaacatgg ttctaagttt acataaagag tagagtcttt taaactttgt
     2881 ttagcactgt ttatagcatt tacttcagca tgattagagc caaatttctc atgatatcct
     2941 ttgcctatta cattactatc tttaacaatt atacatccaa ctaatggatt tggatttaca
     3001 aagccttctc catttttagc caattcaata gcttttttca tataataaat atctttttct
     3061 ttttgattca caaaactcac aatatgtaca cctcctgttt aattggataa tttttaaaca
     3121 aaaaaccctg agaattaact cagggtttag aataatctac aataaatgtt taataagtgt
     3181 agaacaaaat tgctctacaa aaacactcat taaatatctt cttccatcca gactttactg
     3241 tcggctttgg aatttaacca aatcaaccaa gttttacctt ggctcgcggg ctataccgcc
     3301 gattgggaat ttcaccctac cccgaagatt aagtattaaa tttatattta gaatgtatca
     3361 taaattattt tactttgcaa actatttttt taaaaaatat atgttaatat ataatataga
     3421 atgtctataa actttgaaat accacgattg tgaatataaa aataaaaatt tgaacataat
     3481 caggaaagaa gtctaattaa tttaaattag gagagtaata tatgaataaa aaacttttag
     3541 aaatactatc aaaatactat ggatatacaa gttttagaaa aggccaggaa tctataataa
     3601 attctatctt aagcgagaaa gatgtgttag ctattatgcc aacaggaggg ggaaaatcaa
     3661 tatgttatca aattccagct ttgattttag atggaatgac aatagtaatt tctccactta
     3721 tatcacttat gaaagaccaa gtagatgctt taaagactat gggaattagt gctgctttta
     3781 taaatagttc attaagtagc aaagaattta atgaaatatt aaataatatt agaaaaaata
     3841 attataaaat actctatatt gctccagaga gattagatgc acaagagttt cttgagctaa
     3901 taaataataa caatgtaagt caagttgcga tagatgaagc acattgtgta tctcaatggg
     3961 ggcatgattt taggttgagt tatagaagga tttctgattt tataaaaaat ttgcctaaaa
     4021 gacctatagt tacagcattt actgcaacag cttcagaaga ggtaagaact gacataataa
     4081 atttactgtg tttagaaaat ccagattatt atataactgg atttgataga gagaatttat
     4141 caataaatat agtaaaatca tctagtaaaa ataagtatat tttagattat attcaaaatc
     4201 ataaaaatga gtctggaatt atatatgttg cgactagaaa ggaagttgaa aatatatata
     4261 atggtttatc taagagaaat atatctgtgt caaaatatca tgcaggttta tctaaaaatg
     4321 aaagaagtaa aaatcaagaa gattttataa atgataatgt ggatataatg gtagcaacaa
     4381 atgcatttgg aatgggtatt gataaaccta atataagatg ggttatacat tacaatatgc
     4441 ctcaaagtat agaaaattac tatcaagaaa taggtagagc tggtagggat ggagaaaaaa
     4501 gtgagtgtgt acttttattt tctccaggag atgtacatat acaaaaatat ttaattgata
     4561 ttggagttga aaatcctgaa agaaaattat ttcagcataa gaagcttcaa tacatgattg
     4621 acttagttta cagtaattca tgctatagaa agacaatatt gagttatttt ggagaaaatt
     4681 atatagacaa ttgcaataat tgtagcaatt gtttagtaga gggtgaaata gtagataaaa
     4741 ctatagatgc acaaaaagtc atttcatgtg tagctagaat gaaaagaagt tatggtgtta
     4801 ctactattgt agatgtactt agaggttcta agaataaaaa gattttacag cttggattaa
     4861 atactttgtc tacatataac attatgagag attattctag tgaagactta aagaatttta
     4921 taaatacatt ggtttctcat ggttttttag atttagttga gactctaggc aatggaaata
     4981 gaggtagttt tcctactata agattaaatg agatgtctat gaaagtttta aaaggtgaag
     5041 tgaaagtaga gtttaaagaa attgttatga caaaatcatt ggaagtagag gataagttat
     5101 ataatgcttt aagagaactt agacattcaa ttgcaagtga agaaaggata gctccatata
     5161 tggtatttgg agatggtaca cttagagcca tgtctagtag ttatccaatt aataaagaag
     5221 aaatgctaga tatctctgga gtaggagaaa ttaaatatca aaaatatggt agagattttg
     5281 aagcagtaat taaagaatat gtcgaaaaaa acaatataaa taaaaaaata gatgaaaaaa
     5341 atgtcgattt aaagaacatc aatagtgatt tcttagaggt taatacagat aaagttttat
     5401 atgaaaaact gttagtcatt agagaagaat atgcaacaaa ggaaaaatta cctgcatata
     5461 tggttttagc caataaggct ttgaaagaaa taagtggaag atatccactt gatgaagaac
     5521 aattaaaaga tatttctggc ataggtggag taaaatttga aaaatatgga gctagtattt
     5581 tggcagaggt taataaatat gtaaaagaaa ataatattaa tgttatttgg gagaaaaagg
     5641 gcaaaaggaa gttaatatta gatggagaaa gtagaaaaaa taatgaaata gcattagatt
     5701 tactaaatca aggaaaagat atacaaagta tcagtcaaga aatagaagtt tcactgtcaa
     5761 ccattattgg atatgtgcat gactatgtaa aggatggaca tagtatatca tttgaattag
     5821 gactagaaaa tttttataat gaagataaaa agaagatgat tttagaagtt tgcgaaaaag
     5881 ttggctatga taatttaaat aacataaaaa gaaaacttcc agattcaata aagtatgaaa
     5941 atataagagc tgtgatactt gactcttatc ttgaaaactt aaaaaataat attatagcag
     6001 agtaattttt aacttaataa aaaataaaat ttgtattatt gtgttgaact aataattttg
     6061 ctgtgttata ctgaatttgt aaaaataatg aaaaattaga gctaggggcg ctagaattgg
     6121 ctagctgaga gataaagccg taacttacaa accctaataa cctgatcaag ataatgcttg
     6181 cgaagggaag ctttttaata taattttatt atataaatag gcaaattatt taatgttagg
     6241 ggaaatttgt ctaagtgtat atacgattat aagagtgctt tccaaacgag gaaaagcact
     6301 ttttttattt tcaaaaacta taactttaat cttggaggat tgtaatatga actatacaac
     6361 acaaatggat gctgctagaa aaggcataat aactaaagaa atggaaatcg tttctcaaaa
     6421 agaacaggta gatgtaaatg aattaagaga attaatagca aatggacaag ttgttatacc
     6481 agctaataaa aatcataaat ctttaagtgc agaaggtgta ggtaaaaatt taagaacaaa
     6541 aataaatgta aatctaggaa tttcaagaga ttgtaaggat atagaaaaag aacttgaaaa
     6601 agttagagta gcaatagaca tgaaagctga agctataatg gatttaagta attatggcaa
     6661 aactagagaa tttagagaaa aagttgttga aatgtcacct gcaatgatag gttcagtacc
     6721 tatgtatgat gcagtaggat atttggaaaa agaattaaaa gatataacag aagaagagtt
     6781 tttaaatgta ataagacaac atgctattga tggagtagac tttattacta tacatgcagg
     6841 tcttacaaga agtgtttgcc aaaaaataaa aaatcatgag agattgactc atatagtatc
     6901 tcgtggaggg tctttattgt ttgcatggat ggaattaaat aataaggaaa atcctattta
     6961 tactaatttt gataaaatat tggatatatg tgaagaatat gatgttacat tgagtttagg
     7021 agatgcttgt agaccaggtt gtataaaaga ttctactgat ggtgttcaaa ttcaagaact
     7081 tgtagtatta ggtgaactta ctaaaagagc atgggaaaga aatgtacaag taatgataga
     7141 aggtcctgga cacatggcta ttgatgaaat agaagcaaat gtagtattag aaaagagatt
     7201 atgccatgga gcaccttttt atgttcttgg accattggtt acagatatag caccaggtta
     7261 tgaccatata acaagtgcaa taggaggagc tttagcatgt gccaaaggag tagatttttt
     7321 atgttatgta actccagcag aacatttaag attgcctaat ttagatgata tgaaagaggg
     7381 tataatagct gcaaagatag ctgctcatgc aggagatata gctaaaaatg ttaaaggtgc
     7441 tcgtgaatgg gataataaga tgagtaaagc tagagctgat ttagactggt gtgagatgtt
     7501 tagacttgca atagaccctg aaaaagctaa gagatacaga gatgaatcaa ctcctactca
     7561 tgaggatagt tgtactatgt gtggaaaaat gtgctcaatg agaacagtta agaaaatatt
     7621 aaacaatgag gaacttaatc taatataatt tttggctagt atgactggaa taaaaataag
     7681 ctgaaaagag gaggtgattt agtgaaggtt aatgggaagg aaattgaatt tgaaaaagat
     7741 ttgactgtta tagatttatt aaataaatat aatttaaaaa gtgatagagt tgtagttgaa
     7801 gtgaatttag agataattga agaatctaac tacaatacat atgtattaaa agacgaggac
     7861 attgtagagc ttattagctt tattggaggt ggttaaaata aaaatatttg taaatgaatt
     7921 attaacagat gttgaggaaa atacaactgc ttataaagtt agagatagct ataacaataa
     7981 atgtgatgta ttggtgttaa atggatatcc aattaaaaca gatatgcccc tttgtgaaaa
     8041 tgataaatta acacttatac aaaaaggagt taagccatca cttgatgaat tggaaagctt
     8101 gttgattgct agacatactc caaatataca taataaatta aaaaaaggga aagttgctat
     8161 attgggattg gggggacttg gttccaatat agctatctct ttagctagaa taggagtggg
     8221 agaactttta cttatagatt atgatgttgt agaaccatct aatttaaata gacagcagta
     8281 ttttatagat gatataggaa aactaaaaac tgatgccatg atagaaaata taaaaaaaat
     8341 aaatccattt ataaaattaa ataaacgaga tattttttta aacaagaaca atatggatac
     8401 tattaaggat tcagatttaa taattgaggc ctttgatgat gcttcatgta aagcacaggt
     8461 atgtaattat gtattaataa atttaaaaga taaatattta atagcatcat ctggtatggc
     8521 aggttactat gattcaaata taataactac taagaagata aaagacaggt tttatatatg
     8581 tggggatttt gtgaatgaag ctaaatttgg tgaagggctt atggctccaa gggttgccat
     8641 ctgcgcaaat catatggcaa atttggcaac gcaaatactt atagatatgt aaatattaaa
     8701 attagatata aaaaagattg aatattaaaa aaatttaacg tggagggaaa taatatggat
     8761 aaattggtac ttggtggaca tgaatttaat agcagattat tggttggaac aggtaaatat
     8821 ggttcaaata atatattgcc tgaggtaata aaagaaagtg gaagtgaaat aataacaatg
     8881 gcacttagaa gagtggattt agacaataaa caagaaaaca tattaacgta tattccaaaa
     8941 gagatgacaa tacttccaaa tacatcagga gctactaatg cagaagaagc tgtaagaata
     9001 gctagaatat ctagaaaaat gggatgtgga gattttataa aaatagaagt aatatcagat
     9061 actagatatt tacttcctga taatgaagaa actataaaag ctacaaagat acttgctgat
     9121 gaggggttta tagttcttcc atatatgaca ccagaccttt atgctggaag aagacttata
     9181 gaagctaatg cagcggcagt tatgccactt ggagctccaa ttggttcaaa tagaggtctt
     9241 caaatgaaag aaatgataag aataatgata gatgagttgg atataccaat aatagtagat
     9301 gcaggtattg gaaaaccatc tcaagctatg gaggctatgg aaatgggagc tgatgcagta
     9361 cttgtcaaca ctgctatagc ttcagcagga gaccctgtac aaatggctag agcatttaaa
     9421 ttagctgtag aaggtggaag agaagcatat attgcaaaaa caggaaatgt gtcagaattt
     9481 gcaaatgcat cttctccttt aacaggtttt ttaggcaatt tatagaaagt ggtgattggt
     9541 gatgagtttt tatgatgtaa tagaaaaata tagggatttt gattttgatg gatatttaaa
     9601 taatgttact gataatgatg ttttaagaag tttatcaaaa gacaagcttg aagattttga
     9661 tatattaaat ttgctttcta aaactgcagt aaaacattta gaagatatgg cacaaaaagc
     9721 acataaatta agtgttcaat actttggtaa aacagtatgt ttatatacac caatgtatat
     9781 agcaaattat tgtgttaatc aatgtgttta ttgtagctat aatataaaga gtggaataaa
     9841 aagaaaaaaa ttaactatgg atgaaattag agaagaggga gaagcaattt caaaagaagg
     9901 tttcaaacat ctacttgttt tgactggaga aagtagtttt cactcaagtg tagaatatat
     9961 aggtgaggct atagaaatat taagagagaa atttccctct ataggtatag aagtttatcc
    10021 aatggaagta gaagaatata agtacattgt agataaaggt gttgaaggat taacagtata
    10081 tcaagaaact tatgacgagg aaatatataa gagagtacat ataaaaggcc caaaatcaaa
    10141 ttataaattt agactagatg ctcctgaaag aggggcaaaa gcaggtatga ggactttatc
    10201 aataggtgca cttttagggc taaatgattt tagaaaagaa acttttttta caatattgca
    10261 tgggaaatac ttaaaaacga aatatcctca tattgagctt tcatattcaa caccaagaat
    10321 gagacctttt aaagggtgtt ttgaggaatt ggtagatata agtgatactg atttagttca
    10381 agctatggta tgtatgagat tatttgaccc tcatgcagct ataaatatat ccactagaga
    10441 aaatttagaa atgagaagtc atataatacc tttaggagta actaaattga gtgcaggtgt
    10501 atctactgat gtaggaggtc attctcaaga tgaacatgat actgctcaat ttaagataaa
    10561 tgatgaaagt actgttaaag atgtggagaa aatgctaaac tcaattggtt atcagcatgt
    10621 gtttaaagat tgggaaagat tttaaatgta tctgataaca aacagaaaat tatgtagtga
    10681 ggaacgatat ttagaagtta ttaaagagtc tatattgagt ggtgtagaaa atataataat
    10741 aagagaaaag gatttagaat atcaagagct aagaaaatta tatatgaaaa taaaaactaa
    10801 aatcaattgt atagattttc aagagcaaat atcagatgaa agtttgaaaa ctaatataaa
    10861 tcaaaaagag tgtagaaata aattcaaggt taattttata ataaatagta acattgaatt
    10921 ttttgaaaaa gtggattgtc aaggtatcca tcttcctttt aaattattct tgaatttaat
    10981 tgaaaataag tataatttta atgaaaataa aatactagga ttatctttac ataaagtaga
    11041 ggaagtagat tatttagaaa aattaataag aaatcaaaat ataaaaatag attacataac
    11101 attatcacat atatatgaaa ctaaatgtaa agaaggatta aatccaaaag gaattgagct
    11161 tttaaaagaa gctaaaaaga ttactgatat taaaataatt gcacttgggg gaattttacc
    11221 atctaatgtc aaggaaacat taaaatactg tgatgatttt gctataatgt caactataat
    11281 gaagtctaaa gatattaaaa aaaccatatc aaattataat gaaaagttga attagtaaat
    11341 atagtatatt gaatttttaa cttaaataaa aataaaaaag ttatctttta aattttaaaa
    11401 ggtagctttt ttattttata tctttgctaa taaaaaattg taaaaaggga aaactttagt
    11461 cagagagatt aaacaatatc ctagaaagat ttaaaattta gtaaagtatg gaggacatta
    11521 taatgactga cagtaaaaag aaacgattta ttataccaca cacttttaca ataatatttt
    11581 tactaatagt cttgatggca atattgactt ggatagtacc aagtggtgaa tttagtagag
    11641 aagatgtaga aggtagaatg gtagttgtac cagggactta tcaaagtgta gaaagtaatc
    11701 ctcaaggatt tgcagatgtt tttaaagcac caatagaagg ctttatcgat tcagctgaag
    11761 ttgtaggttt tgttttaata gttggaggtg cttttggtat tgtaaatagg actggagcta
    11821 tagaagcagg aatagcttcg gttatctcta agtttaaggg aaaagaactt cttataattc
    11881 caatatcaat gattttattt ggacttggag gtacaacatt tgggatggca gaagagacat
    11941 taccatttta tatgatattt gttccactca tgacatcctt gggatatgat tctctaacag
    12001 caatagcaat tgtatttata ggagcagcag caggagccgc cgcttcaaca ataaatccat
    12061 tttcagttgg tattgcacag gcactagctc aacttgctcc tggttcagga gtaacttaca
    12121 gagtagttat ttttatagca tatatactaa taagtatagc atttgttatg tactatgcta
    12181 agaaggtaaa ggaaaatcct gaaagttcaa tagtatatga aatagataaa aataataaat
    12241 caatacttaa tcaaacttca acagaaataa aagaatttac ttttaaagaa gtatctgttc
    12301 ttgctatatt tggaataggg atggcaataa tgatttgggg tgttttaagt caaggatggt
    12361 acattccaga aatatcaatg tgttttgctg caataggtgt gctttctgga ttggttggta
    12421 gattatctca aattgaaatc tcagaaagtt ttatagatgg agcaaaagat ttagcatctg
    12481 cagctcttgc tatagctttg gctcgtggaa tcgtaataat tgctcaaaat ggatttataa
    12541 tagatactat attaaattcc gcagcagcct ttctaggtgg gcttccaaag gttatatttg
    12601 tatatttatc atttttcttg gaagctgcat tggcattttt aattccatca tcttcaggtc
    12661 ttgcttcact tacagtacct gtacttgctc ctcttggtga tttggttaat atatcggctc
    12721 aagtgatagt aaccgcttat caatttggtg tgggtcttac aaactttata actccaactt
    12781 caggagtact gatgggagca cttgcagtag cccatattcc attttcaaaa tgggtaagat
    12841 ttataatgcc tatcttgttg attttgacag ctgtatctct agtcttttta acaataggta
    12901 tttatatagg gttttaatta agtgtagttt tatttaagat atttagtaat ggtataaaat
    12961 gataatatgt tttcaataaa ttgccactaa tttaattagt ggtaatttgt tttttgtatt
    13021 taaaaataaa tatatttact tgtattttga attaatatct acttagacat tgttttaatt
    13081 gttatatttt catttaatta agcattgatt tatcattttt tatatttcat ataacaatgc
    13141 aaaaaataat ttttatatag taaatgacaa aagtttacaa taaatgtata ttttcttact
    13201 cattccttaa aattactcat ttagtttaat aactctcatt tcatatgtaa agttggtaaa
    13261 aaaattaaaa ttaaaagtaa aaaactaaat aaattaatgt taatttgaaa tatatatgaa
    13321 ggtttttgga gaatacttac ttatgatagg aaaaaatgtt aaaataatag tgtaatcaaa
    13381 tataaaggac tgaaataatt aacagttatt aaatattagg agtgtaaaaa tttatgggat
    13441 taaaagaatt tgattttatt gaagagtcta ttgatatgtt gaggacaatg tcaccaacat
    13501 tagaaactat atcagatgaa atagaagagt attttgaaaa tatcttggat gaaaagaatc
    13561 aagaatatat aaatgtaacc tctagaataa aatcagaatc aagtttaaga gaaaagataa
    13621 ttagaaatag atatttaaaa aaatatggag aagcaagtaa tcttattcat aatgtttctg
    13681 acttaatagg cctaagaatt gagtgtagat ttattgagga tgaaaacaaa atatatagac
    13741 ttttgagaag atattttaat aaaacagatg acaaaataaa ctattataat aaagagaata
    13801 agaatattaa attaaaacta tctgagagac aacccaataa gcaaaaaaat ggatttgaaa
    13861 tatataaaat agatggagtt tttgcgtatt tagatagaga agtaaaattt gaacttcaaa
    13921 ttaaatctct tgtaaatgtg ttttggagtg aaatagaaca taaaataatt tataaaaaca
    13981 atacatattt acttgctgat aaatttataa aggatatgat ggactctata aagaataatc
    14041 ttactatgat agataatcag cttttaagta tttataaaaa ctttcattca gggaagtctt
    14101 ttaatatgaa agtaagtaaa aaagaaattg aaaaattatt tgcgaaacta gtctatgacg
    14161 ctttttctga aaagatgaat aaaagtatag gttttgttgt agattttaaa aaaccatgtg
    14221 agactatatt gaactattct tttaataaac aagatataag tgatgaagtt ttaggtaatt
    14281 ttatgcttga tgagtttgca agattaaatg aaattgtaaa caaggatata gattttaatg
    14341 agcaaataga atttgaaaga gaaccaacat ttgatgataa gttttgtaaa gacctaggaa
    14401 atcattttag aagtagatta aacacagaat ttccatggaa cctatttttt agaatattat
    14461 ttgaaataga gccatataac aataccaaag actttgaaaa ctttgtagag tttataaaag
    14521 aaaatgtgct actagaagaa aatagggagc aattattatg ccaatttgaa gaagatagca
    14581 aacttataat tgaagatata tatgattgta tatttaatag tataagtgag attgatagtg
    14641 ttgaaatttt atataactat aatcttgaaa aaataaactt tacatcaagt gaaatcataa
    14701 attgtatatg tagagaatat gagtgttatg gtgaatactt agaagaaaaa gaaaagctta
    14761 tgaatacatt taaagaaaag attattcaaa tctttgagta ataggtcaat gctgaagtgg
    14821 cgctaatgcg ccatttttca aatatatatg aattaagtaa aattattttg gaggaggcga
    14881 tttgtataga gaaaacaggt gtaattttgg cagggggaag aaattctaga atgggtagag
    14941 ataaagcctt tttagagtta catgataagc tttttattga aattgcaata gaagcattta
    15001 aaaactttga tgagttgata ataatatcta ataatgaaga attatattct aaatacgata
    15061 ttaaagttta taatgatata gtaaaagatg ttggtccaat tggaggaata tatactgctc
    15121 ttgaatatgc aaagtatgat atagttacaa ttgcatgtga tatgccatat ctaaataatc
    15181 aaatagtcga aagaatagct aataaaatga atgataaaag tgtgatttca gtgacaaatg
    15241 gaaagttaca accattgtgt tctggatata aaaagagtat aataaataaa gtatctttat
    15301 gtataaggga aaatgattta aagctcagaa gctttataga taaaatagat aaaagttata
    15361 tatactttga tgaagaagat ttatttttaa atgtaaacac tgttgatgag tatgaaaaat
    15421 taactaaagt ttaattagct atggttgtaa aatagagtag gatttatatg gaggagagtt
    15481 tatgaattta cttaaatgcg attcttgggc agtaatatta aataatagcg ataaagaaag
    15541 taaagcatat aaaatattag atgaacttaa aaggaatatg tacaaagttg ttgctataga
    15601 tgaagagaaa aaacctattg aaggaataga tgtgtatgaa tgtttaaaag atgttccaca
    15661 caatattgat gttgtagcta ttattgataa acaatctaag atggatgtga ttttagaaga
    15721 agtagagctt ttggatatac aaaatatgtg gtttgaaaaa ggaagcttta gtgaaatgat
    15781 gattaaaaag actaaagatt taaaattaaa tatagaatat aatttaagtt tatatgatga
    15841 gttgactaga taaaattata tatcttaata ataaaaatat aattagttaa taaaacatat
    15901 tattttatag tatagattgg ttaataaaag tacagattgg ttaatgaaac attaaattac
    15961 aatatttgtt aaaaaaattg tgagttacat gatataatgt attaaatagg atactaagga
    16021 gttgatatat tatgttaata gtaactacag aaaaagtaga aggtaaaaag atatcaaagg
    16081 ttttaggatt agtgagagga agtacaataa gagcaaaaca tgttggaaaa gatataggag
    16141 caagttttaa gaatcttgtt ggaggagaac ttactggata taatgaaatg ctcactgaag
    16201 caagacaaat tgccataggt agaatggttg aagatgcaga agctaaaggt gcaaacgcag
    16261 taatagcatt tagactgtct tcagcttcag ttatgcaagg ggcagcagaa atgcttgctt
    16321 atggaacagc agttgtttta gaagatgata atagtattct tgaaaaataa aatataaata
    16381 gttaaaaaga ggcatctttt aaaaatttta tggatgcctt tttttatttg aaaaaactaa
    16441 tttgagaaga aattttaact attatgacaa taaaattagg gtgatattat ataaataagt
    16501 aaaattatat tatataatgt atggtataga ctttaaaaat tttatttgta gagaaaaata
    16561 tggacaatgt tataataatt atagaaatta aaagtactta cattcattat gtatgtgaca
    16621 tgtataatta gttttatatt taatacagtt ttgaagtaat atcttcattt tgagaacgtt
    16681 ggaataataa aaaatgtatt attataggtg atataaataa aaaataaata taatatgtta
    16741 tattgctgat agaaaagaga ataaaagaaa ataggtttta ttaaaagaga tttttattta
    16801 taggacttaa tcaatgtata gaggggtgag aacatatgtt taatgttgca attataacac
    16861 ttagtgataa aggatatgaa ggaaaaagag aagatataac aggaaaaaag ttaactgaat
    16921 ttgttgaaaa tacaggagct tataaagtta ctgaatatgt acttataaaa gatgataagg
    16981 aaatgctaaa ggaaaatatt ataagacttt gtaatagtaa taaaatagat ttaattctta
    17041 caaatggagg tactggtttt tcaaaaagag atataacccc agaagcaact aaggaagtgt
    17101 tggaaaaaga aataccaggt ctgagcgaat atatgagaat gaaatccaca gaaattacta
    17161 aaaaggccat actaagtagg ggtgtaagtg gaataagaaa taattctata ataataaatt
    17221 tacctggaag tcctaaaggt gcagttgaaa atctaagttt tataattgat gttttagacc
    17281 atggcataga agttttaaga ggagaagcta cagaatgtgc cactaaaaaa agtgaatagt
    17341 atataatcta gatagataaa aaatagacaa agaggtagat aaagtatgat tgataagtat
    17401 ggaagaaaaa tagactacct tcgtatttca cttacagata agtgtaattt aaggtgtgtt
    17461 tattgtatgc caccagatgt aaaatttgat aaaaattata taaatgaaaa tctaagcttt
    17521 gaagactaca agtttattat aaaggctatg gctgaacaag gtataacaaa aattagattt
    17581 actggaggag aacctctttt atatcctaaa ttaaatgagc ttataaaatt tacaaaagaa
    17641 gaatgtggaa taaataacat aggaatgacc actaatgcta ttgggctttc tgatagaatc
    17701 tttgagcttg aaaaatgtgg cttaaataaa gttaatataa gtttagattc tttgaaggaa
    17761 tataagtata aatccgtaac aagaggagga aacttaaaag atgtcttaaa atcgattgaa
    17821 tcttgtttaa acttaggtat aaaagtaaag ttaaattgcg ttgctataag tgggtttaat
    17881 gatgatgagc ttaaagattt tatgttttta acagttaaat caccaattga tgtaagattt
    17941 atagagctta tgccattagg tgaggctaat agagtttata agaagggtta ttttaatgta
    18001 aaagaaatga ttgaaaaaat atatggttta tataaagtca aaaatgatga aaaaagtaca
    18061 gcagaatatt atatgtttaa aggagctaaa ggaagaatag gaattataac tccattaagc
    18121 tgttcatttt gtaacacttg taatcgtatt agacttactt ctagtggtgc aataaaatta
    18181 tgtctacatt caaaagaaga aatagatata aaaccatttt tacataaacc attgttattt
    18241 agagaatcta tgaaagatat aataaaacaa aaaccagaaa aacatcattt aatagaaaat
    18301 aaatgtagtg atacaaatag gcgtatgtat gaaattggag gataagtaat gttaacacat
    18361 ataaatgaaa aagggtatgc caagatggtg gacataagtg aaaaagatga tactaaaaga
    18421 acggccgtag ctacagctac aattactctt agtaaggaag cattagaaaa agtaaaaaat
    18481 ggtcagataa aaaaaggaga cgtattgtct gtagctcaag ttgcaggtat aatgggagct
    18541 aaaaatacat cttcaaacat accaatgtgc catcagatta aattagttgg atgtgattta
    18601 gaatttgatg tagatgattc tatgtctgct attaagatat acgcaacctg taaatctctt
    18661 gggaaaactg gagtagaaat ggaagcttta aatgcatgtt ctttagcagc acttacaatt
    18721 tatgatatgt gtaaagcagt tgataaaaaa atggttataa gtaatatcca tcttgtagag
    18781 aagactggtg gaaaatcagg agattttaag ttcgagtaaa ttaataatat attgggttaa
    18841 aatttggagg aaatatggcg aaggtagtgt caataaatat aagtgagaaa aaaggtgtaa
    18901 ttaaagttcc tgtacaatgt gcagagctaa aaataaatca tgggatagtt ggagatgcac
    18961 atgcaggaaa ctggcatcgt cagataagct tacttggaaa agaaagcata gataaaatga
    19021 aaatgaaagg ctttgaccaa ttaaaatatg gagattttgc tgaaaatgta actacagaag
    19081 ggatagaagt tttttccctg cctgttggta caaagttaag aatagggcaa tgtgaagttg
    19141 aagtaactca aatagggaaa aaatgtcaca atggatgcga aattaagaaa ttgacaggtg
    19201 actgtgtgat gccacgagaa ggaatttttg taaaggttct aaaagaagga attataaatg
    19261 tgaatgattc aatagatgta atctaatgaa gtcactatta aaatataatt ataagtaaat
    19321 actattaaga tttaaatata tattttaaga gagaattaat tttaataaaa tattttagtt
    19381 ata
//
LOCUS       DAEORZ010000070        19352 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEORZ010000070 DAEORZ010000000
VERSION     DAEORZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.070"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(383..1375)
                     /locus_tag="KRH39_002200"
     CDS             complement(383..1375)
                     /locus_tag="KRH39_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8512099.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(1384..2442)
                     /locus_tag="KRH39_002201"
     CDS             complement(1384..2442)
                     /locus_tag="KRH39_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG8512100.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            2567..4030
                     /locus_tag="KRH39_002202"
     CDS             2567..4030
                     /locus_tag="KRH39_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBG8512101.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            4132..4929
                     /locus_tag="KRH39_002203"
     CDS             4132..4929
                     /locus_tag="KRH39_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8512102.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            4942..7044
                     /locus_tag="KRH39_002204"
     CDS             4942..7044
                     /locus_tag="KRH39_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBG8512103.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            7112..7591
                     /gene="rlmH"
                     /locus_tag="KRH39_002205"
     CDS             7112..7591
                     /gene="rlmH"
                     /locus_tag="KRH39_002205"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBG8512104.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSRMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            7745..9163
                     /locus_tag="KRH39_002206"
     CDS             7745..9163
                     /locus_tag="KRH39_002206"
                     /inference="COORDINATES: protein motif:HMM:NF012376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG8512105.1"
                     /translation="MSKQLNWVHLSDIHFTYKDYCTNTMRDLLLKYLKSLSSDKIFDF
                     IVITGDIVFQGAKYDNDLINFINKILSALNLTKSELFIVPGNHDLKRDKMTSLALENL
                     AREKGLKASIDKELEDGFKKRQRAYFTFLKNVTDNSYTNTKLHTVKSLDKCNLIHLNT
                     AWSCGNRYEEGNLRIGLSRLTEVLKEVEGSEKINIAIGHHTIECFHESERDKIINRFV
                     DQDVDIYLAGHVHKPKYHFEANNNRVIPMFVCGSGMVQDFGTNNFIVGSIDLETKQGK
                     ITYHSWNNDLEKWDVDSNVGRQVCKGDQKFDIDRLKKKDNISNILEKEYIDTDLDENE
                     FKEFLIEFHSQFKYNGSQEFNQIEVDIKEKFVNMKCINSVEKEFLKFSAKFPIVNQIM
                     NNPLYLDGEAKLIIPSVIVTEYQKVFDDYPTGTKIIEAMVDNMYNQYKNMLKYPKVKL
                     CIYLKIMVYWSIQECDIFNDFK"
     gene            9176..9415
                     /locus_tag="KRH39_002207"
     CDS             9176..9415
                     /locus_tag="KRH39_002207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512106.1"
                     /translation="MNYFKLTKHQVIDENIAYTAMYLYKLIDREMYLDELFQKYVNSK
                     GTQLSSAIEKTLLLSLTFLFGLGKINIIESMIVRN"
     gene            9418..11187
                     /locus_tag="KRH39_002208"
     CDS             9418..11187
                     /locus_tag="KRH39_002208"
                     /inference="COORDINATES: protein motif:HMM:NF021594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HBG8512107.1"
                     /translation="MILKELIIINLIQKKVLKNYVFKDAGINLILGLKRCENDESNGV
                     GKSDMVDSIRYILGRSRPSQFDNNILKEKDIFIVLKIQLEKEKTVYLGRRILEENKGY
                     VLENKGLLLDISSWELKESDSYKLYIQDIIYKNDFKNENPSISSVMEYVIRDEKNGFN
                     EIKLSDRKAYESYRILDFLCELPYNLERNIKTLKDQQRDFNKQLAVIKSMEDEILDLK
                     VRKKRLEEEISELGRIIKDVDISSKFDVDGDKYKKYKKELNKVQGNIFELEFILKQYR
                     QNILDLEEKVDKIRLVEGLEDFYDQIIGYFPEQLKKNYEEMKSFYEFMLDNRGSYFRD
                     KIKEIEIELEYLYKEKLSLKSIIIETSKILKNTELVDDINNLSENLIDKNKELAEINI
                     KIETYDKKTEINKNINKLKTEILRLNQENEKLVELYGDHKEFLEETFLKLVGIAYSEG
                     EGFLKFEYDNKTTLNATTGRIKINCKIVDEKSHGRLYMKINLFDLTWFLGRIKNNSDI
                     QFLIHDGSYCKPDPMVKQRVIDYIERCLQEYNRGQYFITANVDELTQDTIDNLRKSKS
                     IVAELDREDDSKNRFFGFKYQNM"
     gene            11288..11620
                     /locus_tag="KRH39_002209"
     CDS             11288..11620
                     /locus_tag="KRH39_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512108.1"
                     /translation="MIINIWKKQDLDLVKELPTEVISAIEDTIEILDENYGTERTAED
                     LGGYVAVVDKAGIEELKQHQLKGILHEYIDEIEGTDYISVLFLCSSDFSIVVISKKEL
                     KDLIEKID"
     gene            11643..11762
                     /locus_tag="KRH39_002210"
     CDS             11643..11762
                     /locus_tag="KRH39_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512109.1"
                     /translation="MKRKYLQKECGEILGIELVDHIIVGDNDTYFSFKENCMM"
     gene            11894..12187
                     /locus_tag="KRH39_002211"
     CDS             11894..12187
                     /locus_tag="KRH39_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512110.1"
                     /translation="MFKTENRYMTRKVAEEIPLEINILLWNLIDKLNVEKGYLQIFEL
                     NPIGEGIVEIIHKQEVPEYKSSLYIHNEEIKENKKLYAIDSLEYSTLMFSEEY"
     gene            12388..12975
                     /locus_tag="KRH39_002212"
     CDS             12388..12975
                     /locus_tag="KRH39_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512111.1"
                     /translation="MINNKSEIINKLFFDELKELVDKYNNIDDETITVIERIDNEIED
                     KYIKEYILKDTNKIDEIIAEYKNNLDIDKIIFFAWYNLNIEETSIDRISNYYNELISQ
                     KYTENDNYLIYQNKDDLKEYTRNELDYMLSTDFHIDRLFDKDTIIDFWLNNTTKEELI
                     KEMMLDDDVEDILDLSPEYAFTLTDGSEYVFSSKE"
     gene            13237..13467
                     /locus_tag="KRH39_002213"
     CDS             13237..13467
                     /locus_tag="KRH39_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HBG8512112.1"
                     /translation="MSEILGEEIVRIIHKHYKGQQINLPMKLYSNEYVEKYIIENFSK
                     KSVREMARDLGYSDKWVGRLIKGISLKEEKAR"
     gene            13493..13705
                     /locus_tag="KRH39_002214"
     CDS             13493..13705
                     /locus_tag="KRH39_002214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512113.1"
                     /translation="MSIFDKISKICNEEKKRAIRAHEQSKNSGRRSQVEDTYNHFMKT
                     LEKTSGIKKEDIISMKVNSVERKKRY"
     gene            13775..14074
                     /locus_tag="KRH39_002215"
     CDS             13775..14074
                     /locus_tag="KRH39_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512114.1"
                     /translation="MSKSISETKYCIVLKQAKIRDGNVGYGIEKIFVKSLNQEEIRFV
                     YFKDTFRKEQQLIVRPLDLTEDDLIKLIKESIKKNIFSKEFVSNLRGILNTKEVE"
     gene            14346..15269
                     /locus_tag="KRH39_002216"
     CDS             14346..15269
                     /locus_tag="KRH39_002216"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512115.1"
                     /translation="MYKDTITLAQLAREVYANTDLGKAGVKMSKTTKDNIIEKLRAIS
                     NSLSIDIEKYHIKNTYKIPRIIADILYIYLTVDSSKGSFISKINTKQYEKITKKEKIE
                     FLDKVITTLENKYKDDENYINIQHDIEHIKNTVMFEIEFSHGEREVIEEIKREITDKI
                     DLCFKEYTDIKPDDGLIRVNDEIYTKSIKFERIISDEKGRLDVLSEYDKIIFIRHLKE
                     RIDQSIEEWKELIRLANEIKNDEIDNIVEFDEYVQNNKIIIEGRELLRKSIDEYKKEA
                     IQKYKNKKIEYNKDMEQILKDIKKEIDSKYK"
     gene            15397..17160
                     /locus_tag="KRH39_002217"
     CDS             15397..17160
                     /locus_tag="KRH39_002217"
                     /inference="COORDINATES: protein
                     motif:HMM:NF017826.1,HMM:NF037377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HBG8512116.1"
                     /translation="MAIKHRKKLGEGIIAKEPKPPDKEVRHELVENNSCKFNQEIEKI
                     KPEIDEKVEKNKYETKNYFSDGQCLYAKSSAKPGNIKVGRHIWAKENHYNIDDKTEKI
                     LLEFMTVRKMEAELLNRHDCLVERNLMKFQDKGVDIVKSNGKHVVECIRECEDSIIKK
                     NIHSKLGFMEYEGKIIYKLYEAIGLNSTYVGKYDIKPKGSYSEYIKMIKNHVIGNVEL
                     EFAMVSGLSAILLGYIGEELGLDSTIIHLVGNSTTGKSTALKLAISSFGYPEVKHNGL
                     YGTYNGTNNALIKKLGGLNGVPYALDEISMSESNNFTKFVYSLANGADKERLNKDSEL
                     MEKESWLTTILSNGEKSITRSSNNNAGVQVRVIEAENFTWTKSPEHAEAINHVILNNY
                     GHLAIEFAQYLLDKNRNEVIRNYKQEKINLYDELRKRNVYDDMSMRRCSKFAIIIQTA
                     FIIEELLDIELDIEGMVDMIVSIERESIKYRNFHKVVMDFIKQYVDRYRNKFDGKSKS
                     NSQEILGKITEKEDYTELQMEQLSFELMIKEGGYEDRKIVLKELKKAGFLDCEKDRLT
                     RSRKNSVGVDTEYIVIKLPKK"
     gene            17252..17440
                     /locus_tag="KRH39_002218"
     CDS             17252..17440
                     /locus_tag="KRH39_002218"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512117.1"
                     /translation="MNYELIEKEMLELSIKEEQEYYDYITLNAYKAKALDLILKEINS
                     CRENINKILDEYCRTSSF"
     gene            17687..17863
                     /locus_tag="KRH39_002219"
     CDS             17687..17863
                     /locus_tag="KRH39_002219"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512118.1"
                     /translation="MRSVSIKNDLNGNMVEMTLKINNEKYKLNLLELDKNYYTEEELN
                     RINDILTEILNWNR"
     gene            18056..>19352
                     /locus_tag="KRH39_002220"
     CDS             18056..>19352
                     /locus_tag="KRH39_002220"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012464.1,HMM:NF019149.1,HMM:NF024800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG8512119.1"
                     /translation="MSKRVAIYCRVSTVEQAEEGYSIDSQYQNIKEYCEREGHEIYKL
                     YEDRGISGKNISNRPGIKQLLEDAKNNKFDLVLSWKLNRLSRKLLDILNIVEFLDKHN
                     VAFRSLTESFETETPSGKLQLNIMGAIGEFERETIADNVRMSLIAKSKEGLWCGGVVL
                     GYDTVEIPNEGKKRKNTKLQINEKEASTVRRIFELYSQGYGYKAVVNRINKEGYKTKR
                     NNEFAVATVKEILKNPIYIGKIRYNVRQDWSKKRRNNINQNPIISDGQHEPIIDMETW
                     NKVQVILKERSKKHNKIYDSEFPLTGILKCPVCGAGMTISRSTSKRKDGTKKVLEYYS
                     CGNWKNKGTAVCRSNSIRVEVADEYVLNKIMTVINNKSILKRVIDNINRNKSSKLQPT
                     IDELGRITNEINKLNSKKNKNIELFEDGILDKDELSTRVK"
BASE COUNT         8169 a         1860 c         3248 g         6075 t
ORIGIN      
        1 aaagtacata aattcaaatt aatataattt tctatgttac aggatacttt taagtatctt
       61 tttttaattt cattttttat tatattaaaa atgaaattta acatacaaca aaattttgaa
      121 ttacttgcaa tgtgatagtt ttaaatataa aatttagaat cattctagta tagtattatg
      181 taatatagca aatttaattt atagtatcaa taaatctaga ctactgagaa tgtaaatgat
      241 ttgttaggtt aatatacatt agatatgtaa atagaaaaat aaaaagtatg gtttaattta
      301 tgtggcataa gttttaaaca gagaagcaag tttgctctta cctctctatt taaaaataaa
      361 aaaatgtttt ttaagggcta ttttatttga atctttccca tctcaaatca tctccagtaa
      421 attctaaaat cctaaactta tctaagattc tagacagagt tctttgagtg taagtatttc
      481 ctatttgaga cggtgttaag ttggttgaaa taatgatttt tttaccagca acaagtcttg
      541 tatttacaat attgaagatt tctccactag taaaagaatt attaagttca gttccaaggt
      601 catctattat taataaatca caatcaaaca agtttttata attatcttca cttatttggt
      661 tatttgtgtc tcttctaaat ttataatctt caagaatatc taaaattcta aaagaagttt
      721 gatagattac aacattgcct ttatctaaca attcctttgc aatacaattg cacatatagg
      781 tctttcctag tccagtagaa ccataaaaca gtagattttc actattatct ttcttaaaat
      841 catgaacaaa attttcgcag atacttaaat tatttagcat attttctcta ggagatattt
      901 ccccatcaga gcctttctta ggagaaaata tattcaagtt aaaattcgaa aaattttcct
      961 gacttaaaat cctatctaaa ttagacattt tataagcttc atttacaatt tcttgtttga
     1021 ggcaagaaca tttttctcca ttaggtaaaa atcctttatc tttacaagaa atacattgaa
     1081 actttatttc caagtaatca agaggtatgt tactttcagc taaaagtctt tctttcttta
     1141 cttttaaaga ctctatattc tctttagtct tctttacaat ttcatcttta gatttgggat
     1201 ttaaaagtac tatcttagct aaactgagac ctattttaga aatttcatca tctatagatt
     1261 ttatctcagg gattctatta tatacctcat tttttctatg ttctaataag agttcattat
     1321 catctcttct cttagcatat ttagcaagta tttttcttat tttatcctca ttcatttaat
     1381 cacctactta aacttttctt tttgactttt tttaataatc tcatcgagct catcagaggt
     1441 atactgagtg aatgtttcgt tgaaattatg aaacttagtt tttttgtaag tattgttgtt
     1501 ttgagtagta ttaatctgct tcttattaat attctcctta actatacgtt cttcctcttt
     1561 ttgctttaaa tcatttaaat tttttatatt tttttcattc caatttttaa ttataccatt
     1621 tatataagat atacttgggt ttgaaatatt cttagactta gaacaagcat ttattatcaa
     1681 atctatatcc atattaaatt tatcaaacca ggtgtccata agttcttttt ctgattttga
     1741 tggttgtctt gaaaatccca attcattgaa aatcgtttta tataaaatat atcgttcact
     1801 tcttaccaaa aagctttctt caacatcttt tggtgtatat aaatttgaat cataccaatt
     1861 tctaataatg ccttcaatat atttaactgg ctttgatgta ccagttttat ctttgacgta
     1921 ttcatatgcg caaagtatca tatcggggct catattgtat ttattcatta tatccataat
     1981 actcatcttt tcacttgggt cgagatatcg cccaactatt ttatttatag aattaaacat
     2041 ttttctaata cttggatttt cagaagtaga tacaacctta tctgtattag attttataga
     2101 tgaattattg cttaatatat tctcaatata gaaatttttt aaatctaaaa attctattga
     2161 ataattaaaa ttatcatatt ctccattgtc atgtattttt ataatcttct tttcttccca
     2221 gaatttccaa gcagataaca catcagacaa tggtatattt aggtttttgg ctattgaatt
     2281 attgtcgaac ttaggattag aagtaatatc acatgcttgt ctatatccta aaagataaac
     2341 ttttacgtat aacccatccg ccattggcat aaatatatct ataaagatat tgggaatagt
     2401 gatttcacct aaatctattt cattgctttc tttaaaaaac attttatcac ctcagattaa
     2461 attatatcat tttttatatt atatttgtct gtaaagaaat aaaaggtcac aagattagca
     2521 tgtaaatatt ttctaaaagt attcattttg aaaaggagga gatattttgg ttataaaatt
     2581 caaccaaaaa gtaagaagaa cattatcagt agcaattcta tttatggtat taataatagg
     2641 ttgctttata gaaaaggatg acagtttaga aacatacaaa gaaaaaacta attcaaaaat
     2701 aaatcagtat aatatggatg ttatatttga tgatgagact aagaggttaa tgtgcaatca
     2761 gaatgttgac tacataaata atacaaaatc aaacatagat aaaatatatt tccatatata
     2821 tccgaatgct ttttccaaga aagattttgc tccttttgaa aaaagtgaaa tgacaagggc
     2881 ttatcctaat ggatttaacg aaggatatat agatataaag aatatattaa ataataatag
     2941 taaaatggag tataaaatta aaggagacaa aaatgacata ttagaaatag aattagggaa
     3001 agagttaaaa ccaaatgaaa gaatgtcatt agatataaaa tataacgtaa aaataccaaa
     3061 ttcaattgga agatttggat atggagaaaa tactataaat gttactaatt ggtttcctat
     3121 agcttgtgta aatgatgaaa ggggatggaa tttaaagagt tatgaaacta ttggtgaccc
     3181 attttatagt gagactagtg attttcatgt gaaactattg attccaaata aatataaaat
     3241 tgctcataca ggaaaactta ttgatgataa acatgataat aagaaaatgc tatatgaaat
     3301 agaagctgaa aacgttagag attttgcatt tatactaagt agcaaatttg atgtaaacaa
     3361 agtagattca aagggagtag ctatcaatac atataattta aataaaaagc tttctaaaaa
     3421 agcaactgaa gtggcaaaag attcaataaa tatatttagt gaattatttg gaaagtatcc
     3481 ttacaaagaa ttttctgttg tagcaagtga cttttttata ggggggatgg aatatcctat
     3541 gcttgttatg atagaccaaa gtttatataa tgaaaaaaat gaatttttac tagaatatgt
     3601 tattgcccat gagacagccc atcaatggtg gtattctgct gttggaaatg atgaaataag
     3661 tgaaccctgg ttagatgaag cacttactga atattctaca atagtttatt ttgaacaaaa
     3721 gtatggtaaa gatatggcaa acaagcttat aaaaactatg gagatacaga caaaaagtta
     3781 tttgagcgaa aatatattta agccagcaaa tgagtataaa aattctactg aatacagctt
     3841 gaatgtttat acaaaaggcg caatagcatt taatgaaata agaaaagaag ttggagataa
     3901 agtattcttt gagactttaa atgagtacta taataaatat aagcataaaa atgctaatgg
     3961 aagagctttt gtggaactat ggaatagtaa aggtgtagat ataaataaga ttataagtga
     4021 atgtaagtaa atagtgaatg taagtaaatt ttgtgcagag tattaaattt tatttaatac
     4081 tctgtttttt tggtataata taatttataa ggcagaaaaa gaggtgaagg aatgctaaag
     4141 tattgttcag tagggagtgg tagtagtggt aattgccact atatagggta taaaaatact
     4201 aatatactag tagatgcagg attaagtggc aagagaataa ctacaggtct taaagacatt
     4261 ggtgtagatg cagataaatt aaaaggaata tttataacac atgagcatgt tgaccatata
     4321 aaaggagcag gtatattatc aagaaagttt gatataccag tatttgctaa tataaaaaca
     4381 tggtgttcta tgaaggacaa attaggtgac atcaaagata aaaatatgaa agtatttgaa
     4441 aatgataaaa cctactcatt aggtgatatc atagtgagac ctttttcaat agaacatgat
     4501 gcgtctgacc cagttggata taatttttat acagagagtg atgaaaaaat gtctatagct
     4561 actgatatag gatgtataac tcaaaatata aaaaaacatc tatataaatc taaattagta
     4621 gtattggaat caaattatga ccctaatatg cttatgatgg gttcatatac atatgcttta
     4681 aagaaaagag taatgtcaag tactggtcat ttatccaatg aagatgcagc taatttctgc
     4741 gttgaactta ttaatgaagg tacagaatct atattattag cacatttgag taaagaaaat
     4801 aattttcctg agttagcgta tgagacttca aaaggagtat tggcatcaaa tgatatagta
     4861 gttggacaag atgtaaaact agatgtacta tcacgaaatg atgtttctaa tgtttatgag
     4921 atgaagtaga ggtgaataac tttgctaaag agaaattttg aaaatgttga ggataataaa
     4981 attttatact ctataaagca gggcatgatt ttagcgatac ctgctattat gactggttca
     5041 acggctcttg taattttgaa tcttccaata aaggcttatc aagaatattt atcaggcttg
     5101 tttaatggag aggttacaaa tattcttaac tttataaatg attctacttt aggaattatt
     5161 tcattaataa tattattaac aataagctat agttatggaa aaatatatgg gagcaagtat
     5221 acagtacttg ttcctatagt tgcaatgtgc tcttttttag tattttcaca tggtaatgag
     5281 cttaattctt atatagaaat atttaagaca aagtggttat ttacatctat aattgtttct
     5341 atgacatctt ctgtattgtt tgtaaaactt acagaatctt ttgttacaag tgtaaaattt
     5401 catacagaag gggctgatgc agactttaat atggtagttt cggctatagt tcctttcata
     5461 atagtagtat ttttgttttc tatttttaga gtgataatga taagtctaat tggcagttca
     5521 aattttcaag atatatttta taatttattt tcaaatgttt ttaataaaat gggaacaaac
     5581 cttatgagtg ctctgttgtt tattttttta atgcacttta tgtggttttt tgggattcat
     5641 ggtagtaatg tattagatac cgtcgcaaaa aatttatttg aaaatagtat ggcaattaac
     5701 ataaatttag ttaataataa tcaattacca acagaaattt ttactaagac cttttttgat
     5761 acaatggttt tattgggagg atgtggctct ttactatgtt tagtaattgc aatttttctt
     5821 agtgaaaaac gtataaatgt aagaaagtta gctaaaattg cttcaatacc agctcttttt
     5881 aatatcaatg agatgctagt tttcgggata ccaattgttt ttaactatat tatgtttgta
     5941 ccttttgtaa ttactcctat aatcttaacc atcacaagtt atgtggctat gtctacaggt
     6001 attgtaccat gtacagtttc agctgtagaa tggacctcac ctatattttt aagtggatat
     6061 atgtctacag gttcaataag aggtagtttg ttgcaagtat ttaatttatg tgttggtgtt
     6121 atgatttata ttccgtttat aaaaatgtcg caaaatagat atacatatat gtttaaaaat
     6181 aatatagaaa atttaacaaa cttattaaaa aggtatgaat taacaggtga acagcctaat
     6241 cttctaaacc ataatggtaa aataggttca atttcaaaaa tgttggcatc agacttgaag
     6301 tttgctatga aaagaggaga aatagagctt ttttatcaac cacaagtcaa ttataatgga
     6361 aatgttgtgg gagcagaggg attacttaga tggaaacatt caataggagg gttcatttat
     6421 ccaccactag ttataatttt ggctaaagaa gaagggttct tagatgagct tggagagtat
     6481 attattaata aagcctgtat tgatttaaag aagagtgaaa agctattgag aaatcctgtt
     6541 aagttctctg taaatatatc tccagaccaa cttgatgacc ctaagttacc agagcaaata
     6601 aaaaatatca ttagtagtaa tgatataaat cctaatatgt taggtataga aataacagag
     6661 caaattgcat tatcaggttc acaaataatt attgaacgta tcaatgcgat acataacttg
     6721 ggaataaagt taattatgga tgattttggg atggggcata gctctcttat atatcttcaa
     6781 aataataact ttgatatagt taaattggat ggttcattag ttaaagagat acttacaaat
     6841 aaaagaagta gtgaaattat aatgactatt gtaaatcttt caaaaaattt agattttgat
     6901 atagttgttg agtatgttga aaatatagac caaagggata agttatatgg tttaggatgt
     6961 gaaatatatc aaggatatta ttatagcaaa gcaatacctt ttgaagagtt tgtagaatat
     7021 gcaaatcaag aaagagagat ataattttat aagagcttgt aaattctagt aatgcataga
     7081 tttgtaataa aattaagttt aggagaaata tatgaatata agtatagtag tagtaggtaa
     7141 aattaaagaa aaatatttaa aattaggtat tgatgagttc aagaaaaggc tttctaagta
     7201 ttgtaagtta gaaattatag agttggatga tgaaaaagct ccagagaatt tgagtgttaa
     7261 ggaaatggaa attataaaag acaaagaagg aaagaaaatt ttagggaaga taaagcataa
     7321 tagttatgtt atagccttgg ctattgatgg taagggttta tcctctgaag aacttgctaa
     7381 gactatgagt gatttagcag ttagaggtaa tagtagtctc tgttttataa taggaggttc
     7441 tctagggctt tctgatgagg ttttaggaag ggctgattat aggttatcct tttctaggat
     7501 gacttttcct catcagatga tgagattgat attgttagaa cagatttata gggcttatag
     7561 gataaataat ggagaaccgt atcacaagta acggagcgtg tttttataat ttaaaactta
     7621 tattctaagt agaatataag tttttttagt tattaaaaaa gaaagtttat ttaggatttt
     7681 aaatataaat tatgtaagat ataataatgt agtaataatg aaaattaaat cggaggaata
     7741 aaatatgagt aagcagttaa attgggtaca cttatcagat atacatttta catataaaga
     7801 ttattgtaca aatacaatga gagatttact attaaaatat ttaaaatcat taagtagtga
     7861 taaaatattt gattttatag taataacggg tgatatagtc tttcaaggtg caaaatatga
     7921 caatgattta attaatttca taaacaaaat attaagtgca ttaaatctta caaagagcga
     7981 attatttata gtaccaggta atcatgattt aaaaagagat aagatgacat cattagcttt
     8041 agaaaattta gcacgagaaa aaggattaaa ggcaagtata gataaggagc tagaagatgg
     8101 atttaaaaaa agacaacgag cttattttac ttttttaaaa aatgtaaccg acaatagtta
     8161 tacaaataca aaattacata ctgttaaatc tctagacaaa tgtaacctaa ttcatttgaa
     8221 cacagcatgg tcttgtggta atagatatga agaaggaaat ttaagaattg gactaagtag
     8281 acttacagaa gttttaaaag aggtagaagg tagtgaaaaa ataaatattg caataggaca
     8341 tcatactatt gaatgttttc atgaaagtga aagagataaa ataataaata ggtttgtaga
     8401 ccaagatgtt gatatatatt tagcggggca tgtacataaa ccaaagtatc attttgaagc
     8461 gaataataat agagtaattc ctatgtttgt atgtggatca ggaatggttc aggattttgg
     8521 cacgaataat tttatagtag gtagcataga tttagaaaca aagcaaggta aaataacata
     8581 ccattcttgg aataatgatt tagaaaaatg ggatgtagat tctaatgttg ggagacaggt
     8641 gtgtaaagga gatcaaaaat ttgatataga cagattaaaa aaaaaagata atatatcaaa
     8701 catattagaa aaagaatata tagatacgga cttagatgaa aatgaattta aggaatttct
     8761 tatagagttt catagccaat ttaaatacaa tggaagtcaa gaatttaacc agattgaagt
     8821 agatataaag gaaaaatttg taaatatgaa atgtattaat agcgttgaaa aggaatttct
     8881 aaaatttagc gctaaatttc caatagtaaa ccaaataatg aataatcctt tatatttaga
     8941 tggagaagct aaattaataa tacctagtgt tattgtaaca gaatatcaaa aagtatttga
     9001 tgactatccg acaggtacaa aaataataga agctatggta gataatatgt ataatcagta
     9061 taagaatatg ctgaaatatc caaaagtaaa gttatgtata tatttaaaaa ttatggttta
     9121 ttggagtata caagaatgtg atatatttaa tgattttaag taacggaggt gttatatgaa
     9181 ttactttaaa ttaacaaagc atcaagtaat agatgaaaat attgcatata cagcaatgta
     9241 tttatacaaa ttaattgata gagaaatgta tctcgatgaa ttatttcaaa agtacgtaaa
     9301 tagcaaagga actcagctta gttctgcgat agagaaaact ttgctactat cattaacatt
     9361 tttatttgga ttagggaaaa taaatattat agaaagtatg atagtgagga attaagtatg
     9421 atattaaaag agctcattat aataaatctt atacaaaaaa aggtattaaa gaattatgta
     9481 tttaaagatg ctggaataaa ccttatttta ggtttaaaga gatgtgaaaa tgatgaatca
     9541 aatggagttg gaaaaagtga tatggtagat agtattaggt atatacttgg gagaagtaga
     9601 ccatctcaat ttgataataa tatattaaag gaaaaagata tatttattgt acttaaaatt
     9661 cagttggaaa aagaaaaaac tgtgtattta ggtagacgaa tattagaaga gaataaagga
     9721 tatgtactag aaaataaagg gttattatta gatataagct catgggagct aaaagaaagt
     9781 gattcataca agttgtatat ccaagatata atttataaaa atgattttaa aaatgaaaac
     9841 ccatctatat catctgtaat ggaatatgta attcgagatg aaaaaaacgg ctttaatgag
     9901 ataaagttat cagatagaaa ggcatatgaa tcatatagaa tattagattt tctttgtgaa
     9961 ctaccatata acttagagag aaatataaag actttaaaag atcaacaaag agattttaac
    10021 aaacaactag cggtaattaa aagcatggaa gatgagattc tggacttaaa agtaagaaag
    10081 aaaagactag aagaagaaat cagtgaacta ggaagaataa ttaaggatgt agatatatca
    10141 tctaaatttg atgtagatgg agataagtat aaaaaatata aaaaagaact caataaagtt
    10201 caaggaaaca tatttgagtt agagtttata ttgaagcagt atcgtcaaaa tatattagat
    10261 ttagaagaaa aagtagataa aatacgttta gtagaaggtt tagaagattt ttatgatcaa
    10321 attataggtt attttcctga acaattaaag aaaaattatg aagaaatgaa atcattctat
    10381 gaatttatgt tggataatag agggtcttac tttagggata agataaaaga aatagaaata
    10441 gagttagagt atctatataa agaaaaatta agtttaaaat ctataataat agaaactagt
    10501 aaaattttaa aaaatacaga acttgtagat gatataaaca acttaagtga aaatcttatt
    10561 gataagaaca aagagttagc agaaataaat attaaaattg agacttatga taaaaaaact
    10621 gaaattaata aaaatataaa taaattgaaa acagaaatat tgaggttaaa tcaagaaaat
    10681 gaaaaattag tagaacttta tggagatcat aaagaattct tagaagagac atttttaaaa
    10741 ttagttggaa ttgcatattc tgaaggagaa ggatttttaa aatttgaata tgataataaa
    10801 acaacattga atgctactac tggaagaata aaaataaatt gtaaaatagt agatgaaaaa
    10861 tcgcatggac gtttatatat gaagataaac ttatttgatt taacatggtt tttagggaga
    10921 ataaaaaata attcagatat tcaattttta atacatgatg ggtcatactg taagccagac
    10981 ccaatggtaa aacaaagagt tatagattat atagaaagat gtttacaaga atataataga
    11041 ggtcagtatt ttataacagc taatgttgat gagttaacac aagatactat agataattta
    11101 agaaagagta aaagtatagt agcagaactt gatagagagg atgatagtaa gaatagattt
    11161 tttggattta agtatcaaaa tatgtaataa tatatatttt aaaatatata ttataattat
    11221 gataaagcta gatgagataa aatatttcat ctagcttatt ttgtttttaa ataaggagga
    11281 aaataaaatg ataataaaca tttggaaaaa acaagaccta gatttagtaa aggaactacc
    11341 aacagaagta atatctgcaa tagaagatac tatagaaata ctagatgaaa actacggtac
    11401 agaaagaaca gcagaagact taggtggata cgtagctgta gtagataaag ctggaataga
    11461 agaattaaaa caacatcaac taaaaggaat tttacatgag tacatagatg agatagaagg
    11521 aactgattac atttcagttc tatttttgtg ttcaagtgat ttttcaatag ttgttatttc
    11581 taaaaaagaa ttaaaagatc taatcgaaaa aatagattag tagaaggata tgattacaat
    11641 ggataaaaag aaaatatctg caaaaagagt gtggagaaat acttggaata gaattagtag
    11701 accacataat agtgggagat aatgatacat actttagttt caaagaaaat tgcatgatgt
    11761 agaaagtctt agctaaaaaa aatatgctag gactttttct gtgttccaaa aagatctaat
    11821 tgtaatttag actaaaaccc cgaaaccccg ttaaatttta aataaagaat aaaaataata
    11881 ggagacaaga aacatgttta aaacagaaaa tagatacatg actagaaaag tagcagaaga
    11941 aataccttta gaaataaata tactgttgtg gaatttgata gataaactaa atgtagaaaa
    12001 aggttactta caaatttttg aattaaatcc tataggagaa ggtatagttg aaataattca
    12061 taagcaagaa gtaccagaat acaaaagtag cctatacata cacaatgaag aaattaaaga
    12121 aaataagaag ctatacgcta tagatagttt agaatacagt actttgatgt tttcagagga
    12181 atactaattt tacacagaag taagagcatt tcaatgttag acaatgaaga taaaaaataa
    12241 aattaaaatt ctctttaata aaaccccgaa accccgtaga aaaataataa aagttaaaaa
    12301 agtaataatt aagatctaat ctaaattaaa tcccgaaacc ccgtaaaaat agaaagataa
    12361 ttaaaaaatt gaaaggtaga aatgactatg ataaataata aatctgaaat aataaataaa
    12421 ttattctttg atgaattaaa ggaattagta gataaataca ataacataga tgatgaaact
    12481 ataactgtaa tagaaagaat agataatgag atagaagata aatacataaa ggaatacatt
    12541 ttaaaagata ctaataagat agatgaaata atagctgaat ataaaaataa tttagatata
    12601 gataagataa tattctttgc ttggtacaat ttaaacatag aagaaacaag catagatagg
    12661 ataagtaatt actacaatga attaatttct caaaaatata ctgagaatga taattactta
    12721 atttaccaaa ataaagatga tttaaaagag tatacaagga atgaattaga ttacatgttg
    12781 agtacagatt ttcacatcga caggctgttt gataaggaca caatcataga tttctggcta
    12841 aataatacaa ctaaagaaga attaataaaa gaaatgatgt tagatgatga tgtagaggat
    12901 atactagacc tttcaccaga gtatgcattt acattaacag atggaagtga atatgttttt
    12961 tcaagtaagg aataaaaaat aataaaaata actttaaaat aacggggttt cggggtttta
    13021 attaattagt taagaaaata aagagtaaaa taattaattt aactaaaaaa caacggggtt
    13081 tcgggatttt acacttagac aagaaataaa ataaggaatt tttcaagaaa taatttcaaa
    13141 gaaactttct gtaagaatag ttattattaa ttagagaatg aaaggaggtg aatgatgaaa
    13201 atagaaaaga gtgattttaa aggaatttac gcagaaatgt cagaaattct aggtgaagaa
    13261 atagtaagaa taatacataa gcattataaa ggtcagcaga ttaatctacc aatgaaacta
    13321 tactctaatg aatatgtaga aaaatacata attgagaatt ttagtaaaaa gagtgtaaga
    13381 gaaatggcta gagatctagg atattctgat aaatgggttg gtagattaat caaaggaata
    13441 agtttaaaag aagaaaaggc aagataaaaa atagaaaagg atggataata acatgagtat
    13501 atttgataaa atttcaaaaa tttgtaatga agaaaagaag agagcaataa gagctcatga
    13561 acaatcaaag aattcaggaa gaagaagtca agttgaagat acatataacc atttcatgaa
    13621 gacacttgaa aagacaagtg gaatcaaaaa agaagatata atcagtatga aagtaaatag
    13681 tgtagaaaga aaaaagagat attaattaaa taaaggatta cataagaaag gaatagtatg
    13741 ttgctattcc tttttgtttt taaggagaag aaaaatgagt aaatcaataa gtgaaactaa
    13801 gtactgtata gtattaaagc aagcaaaaat aagagatgga aatgtagggt atggaataga
    13861 gaagattttt gtaaagtcat taaaccaaga agaaataagg tttgtatact ttaaagatac
    13921 ttttagaaaa gaacagcaac taatagtaag acctttagat ttaacagaag atgatttaat
    13981 taagttaatt aaggaatcca taaagaaaaa tatattttca aaggagtttg taagtaatct
    14041 tagagggatt cttaatacta aggaggtaga ataacatgac actagagtta tttgtagcag
    14101 gtattaaatt tggagtgtca atttttggta tagcaatgaa acagagaaaa gaatatacaa
    14161 agtaaaggag atatgataga atgaaagaat ttacgttagg ttcagtaata ggattaggag
    14221 tgtgtatagt aattgaattg atgatctcta gtaaatctat gttagtataa aatataatat
    14281 aataagataa ggttaattaa attttagcct tatctttttt tgaataataa tataggggtg
    14341 attaaatgta taaagataca ataacattag cacagttagc acgagaagta tatgctaata
    14401 cagatttagg aaaagctgga gtgaagatga gtaaaactac taaagataat attatagaaa
    14461 aattaagagc tattagtaat tcattaagta tagatattga aaagtatcat attaaaaata
    14521 catataaaat accgagaata atagcggata tattatatat atatcttaca gtagatagtt
    14581 ctaaaggtag ctttataagt aaaataaata cgaaacagta tgagaaaata actaaaaagg
    14641 aaaaaattga atttctagat aaagtaataa caactttgga aaataaatat aaagatgatg
    14701 aaaattatat aaatatacaa catgatattg aacatattaa gaatacagtt atgtttgaaa
    14761 tagaattttc tcatggagaa agagaagtaa ttgaagagat aaaaagagaa attacagata
    14821 agatagattt atgtttcaaa gaatacacag acataaaacc tgatgatgga ttaattaggg
    14881 ttaatgatga gatatatact aagagtataa aatttgagag aataataagt gatgaaaaag
    14941 gcagattaga tgtattatca gaatatgata aaataatatt cattagacat ttaaaagaaa
    15001 gaatagatca atcaatagaa gagtggaaag aattaataag attagcaaat gaaataaaaa
    15061 atgatgaaat agataatata gttgaatttg atgaatatgt tcaaaataac aaaattatta
    15121 tagaaggaag agaattactt agaaaaagta tagatgagta taaaaaggaa gcaatacaaa
    15181 agtataaaaa taaaaaaata gaatataaca aagatatgga acaaattcta aaagatataa
    15241 agaaagaaat agatagtaaa tataaataat taaaaagata agtattttta cctaagcaag
    15301 aaaagggggg ttaaaatact tatctttttt ttagggttcg actaaagaat atatataaaa
    15361 taaaataaat atatattcga aaggagatga gtaaaaatgg caattaaaca cagaaaaaag
    15421 ctaggggaag gaataatagc aaaagaacct aaaccaccag ataaagaagt aagacatgag
    15481 ttagtagaaa acaattcatg taaatttaat caagaaatag aaaaaataaa acctgaaata
    15541 gatgaaaaag tagaaaaaaa taaatatgaa actaaaaact actttagtga tggacaatgt
    15601 ctatatgcta aatcatcagc taaaccagga aatataaagg ttggaagaca tatttgggct
    15661 aaagaaaatc attacaatat agatgataaa acagaaaaga ttctgttaga gttcatgaca
    15721 gtaagaaaaa tggaagcaga attacttaat cgacatgact gtttagtgga acgtaatcta
    15781 atgaagttcc aagataaagg tgttgatata gtaaaaagta atggaaaaca tgtagttgag
    15841 tgtataagag aatgtgagga ctctattatt aagaagaata ttcattccaa gttaggattc
    15901 atggagtatg aaggaaagat aatatacaaa ctatatgaag ccataggact aaattcaaca
    15961 tacgtaggta aatacgatat taaaccaaaa ggttcatata gtgaatatat taagatgata
    16021 aaaaatcatg tcataggaaa tgtagagtta gaatttgcaa tggtaagtgg tctttcagct
    16081 attcttttag gatacatagg agaagaatta ggacttgatt ctacaataat acatttagtt
    16141 ggaaattcaa ccacagggaa atctacagca ttaaagttag caatatcaag ttttggctat
    16201 ccagaagtta aacataatgg actgtatgga acatataacg gaactaacaa tgctttaata
    16261 aaaaagctag gtggtttaaa tggtgtaccg tatgcattag atgaaatatc tatgtcagaa
    16321 agcaataact ttactaaatt tgtatatagt ttagctaatg gagctgataa agaacgttta
    16381 aataaagact ctgaactaat ggaaaaggaa tcttggttaa caacaatact atctaatggt
    16441 gaaaagtcta taactagatc ttctaataat aatgcgggag tacaagtgag agtaatagaa
    16501 gcagaaaact ttacttggac taaaagtcca gaacatgcag aagctataaa tcatgttatt
    16561 ttaaataact atggacatct agcaatagaa tttgcacagt atttattaga taaaaataga
    16621 aatgaagtaa ttagaaatta taagcaagag aaaatcaatc tatacgatga acttcgcaaa
    16681 agaaatgtct atgatgatat gtcaatgaga agatgtagca agtttgctat tataatacaa
    16741 actgcattta tcatagaaga gttattagat atagaactag atatagaagg gatggtagac
    16801 atgatagtta gcatagaaag agaatcaata aagtatagaa acttccacaa agtagtaatg
    16861 gacttcataa aacagtatgt agataggtat agaaacaagt ttgatggaaa atcaaaatct
    16921 aactcacaag aaatattagg aaaaattaca gagaaggaag actatacaga acttcaaatg
    16981 gagcaacttt cttttgaact aatgataaaa gaaggaggat acgaggatag aaagatagtt
    17041 ctaaaagaac taaaaaaagc aggatttcta gactgtgaaa aagatagatt aacgagatct
    17101 agaaaaaata gtgtaggggt tgataccgag tatatagtaa taaaactacc taaaaaatag
    17161 gtaacataaa taatgtaaaa taaatcagct actaggataa ataaaagtta atttcctagt
    17221 agtataaaat aataaaggag tgtgtttagt tatgaattat gaattaatag agaaagagat
    17281 gctagagtta tcaataaaag aggagcaaga atattatgac tatattactc taaatgcata
    17341 taaagcaaaa gcattagatc tcatacttaa agaaataaat agttgtagag aaaatataaa
    17401 taagattcta gatgaatact gtagaactag ctctttttag agttagttct aaggtgaact
    17461 taaaatatat attataaatt tagttcaata aatacaggag gagacatgga tacagttact
    17521 ttagaacaag taaaagcaca agaaatacta gaaatactaa gtaatataat acaaaggtca
    17581 gtagcaaaaa aagaagagat tgagaagtaa agtagttatc atatattaaa taaaaatata
    17641 gaactatctt gtatttttaa atctaaatat gtaggagatg attaaaatga gaagtgtatc
    17701 aattaaaaat gatttaaatg gtaatatggt tgaaatgact ttaaagataa ataatgaaaa
    17761 gtataaattg aatcttttag aactagacaa aaattattat acagaggagg aacttaatag
    17821 aataaatgat atattaactg aaatattaaa ttggaataga taaccatttt aaattactca
    17881 aaaataggaa atgtaataaa atgaaactac atttcctttc taatacctag aatagtctta
    17941 ggtagtaatt gtgagtaagt caaaatttaa attaagtaaa tataaatagc aagagatact
    18001 aagtgataga ataaaaaatc catagtaaaa aaagaggaca aaggagataa agtaaatgag
    18061 taaaagagta gcaatatatt gcagagtatc aactgtagaa caggctgaag aaggatattc
    18121 aattgatagt caatatcaaa atataaaaga gtattgtgaa agagaaggtc atgaaatata
    18181 caaactatat gaagatagag gtataagtgg taaaaatata tctaatagac caggaataaa
    18241 gcaactacta gaagatgcga aaaataataa atttgattta gttttaagtt ggaaattgaa
    18301 tagactatca agaaaactat tagatattct aaatatagta gaatttttag ataaacataa
    18361 tgtagcattt agatctttaa ctgaaagttt tgaaacagaa actccttcag gaaaacttca
    18421 attaaatata atgggagcaa taggtgagtt tgaaagggag actatagctg ataatgttcg
    18481 tatgtcatta attgccaaaa gcaaagaagg tctttggtgt ggcggtgtag tattaggata
    18541 tgatacagtt gaaatcccaa atgaaggaaa aaaaagaaaa aacacaaagt tacaaataaa
    18601 cgaaaaagag gcaagtacag taagaagaat atttgaatta tatagtcaag gatatggata
    18661 caaagcagtt gttaacagaa taaataaaga aggttacaag accaaaagaa acaatgaatt
    18721 tgcagtagca acagtaaagg aaatattaaa aaatccgata tacataggaa aaataagata
    18781 caatgtaaga caagattgga gcaagaaaag aagaaacaat ataaatcaaa atccaataat
    18841 ctcagatgga cagcatgaac caataataga tatggaaaca tggaataagg tacaagtaat
    18901 actaaaagaa agaagcaaga aacacaataa aatctatgat agtgaatttc ctttaactgg
    18961 aatacttaaa tgtcctgtat gtggcgcagg aatgacaata agtagatcaa ctagtaaaag
    19021 gaaagatgga actaagaagg tcttagaata ctattcttgt ggaaattgga aaaataaagg
    19081 cacagcagta tgtagatcta attcaataag agtagaagtt gcagatgaat atgtattaaa
    19141 taaaataatg acagtgataa ataataaatc tatactaaag agggtaatag ataatataaa
    19201 taggaataag tctagcaaat tacaacctac tatagatgaa ttagggagaa taacaaatga
    19261 aataaataag ttaaatagta agaaaaataa aaatatagag ctatttgagg atggaatact
    19321 agataaagat gaactttcaa cgagagttaa gt
//
LOCUS       DAEORZ010000071        19343 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEORZ010000071 DAEORZ010000000
VERSION     DAEORZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.071"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            157..999
                     /locus_tag="KRH39_002221"
     CDS             157..999
                     /locus_tag="KRH39_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG8512120.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            1014..1478
                     /locus_tag="KRH39_002222"
     CDS             1014..1478
                     /locus_tag="KRH39_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512121.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            1465..2637
                     /locus_tag="KRH39_002223"
     CDS             1465..2637
                     /locus_tag="KRH39_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG8512122.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIVLKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            2681..3616
                     /locus_tag="KRH39_002224"
     CDS             2681..3616
                     /locus_tag="KRH39_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBG8512123.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(3890..6583)
                     /locus_tag="KRH39_002225"
     CDS             complement(3890..6583)
                     /locus_tag="KRH39_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG8512124.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVEPEVAYVVTDMLESVITEGTGGVAALPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            6797..7495
                     /gene="yunB"
                     /locus_tag="KRH39_002226"
     CDS             6797..7495
                     /gene="yunB"
                     /locus_tag="KRH39_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBG8512125.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(7707..8714)
                     /locus_tag="KRH39_002227"
     CDS             complement(7707..8714)
                     /locus_tag="KRH39_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG8512126.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            8942..9826
                     /locus_tag="KRH39_002228"
     CDS             8942..9826
                     /locus_tag="KRH39_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8512127.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            10028..11578
                     /locus_tag="KRH39_002229"
     CDS             10028..11578
                     /locus_tag="KRH39_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG8512128.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            11607..12254
                     /locus_tag="KRH39_002230"
     CDS             11607..12254
                     /locus_tag="KRH39_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBG8512129.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKLFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(12396..12656)
                     /locus_tag="KRH39_002231"
     CDS             complement(12396..12656)
                     /locus_tag="KRH39_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBG8512130.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMSKFQDLVKITSKK"
     gene            12873..14156
                     /gene="hflX"
                     /locus_tag="KRH39_002232"
     CDS             12873..14156
                     /gene="hflX"
                     /locus_tag="KRH39_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG8512131.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            14164..14841
                     /locus_tag="KRH39_002233"
     CDS             14164..14841
                     /locus_tag="KRH39_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBG8512132.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            14927..15370
                     /locus_tag="KRH39_002234"
     CDS             14927..15370
                     /locus_tag="KRH39_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG8512133.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            15675..16319
                     /locus_tag="KRH39_002235"
     CDS             15675..16319
                     /locus_tag="KRH39_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBG8512134.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(16405..16782)
                     /locus_tag="KRH39_002236"
     CDS             complement(16405..16782)
                     /locus_tag="KRH39_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG8512135.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(16782..17168)
                     /locus_tag="KRH39_002237"
     CDS             complement(16782..17168)
                     /locus_tag="KRH39_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG8512136.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            17347..18102
                     /gene="nadE"
                     /locus_tag="KRH39_002238"
     CDS             17347..18102
                     /gene="nadE"
                     /locus_tag="KRH39_002238"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBG8512137.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            18185..18907
                     /locus_tag="KRH39_002239"
     CDS             18185..18907
                     /locus_tag="KRH39_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG8512138.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVTE"
BASE COUNT         7297 a         2325 c         3340 g         6381 t
ORIGIN      
        1 atatcatgaa tctataatat ttttttgatt cataactaaa agtatataaa aatgatgaat
       61 aaatatatat tatacattat caatgatagg ttatataata aaatcactaa caattatatt
      121 ataagaaaat tataaaaatt acaaggaggt atagaaatga aagaaaaagc agggattaga
      181 aatgccataa tagcagcctt tataatcaca atattggcag aattaattgg acctatttca
      241 tttaaagtta tgggaattaa tgtaacttta cttagtatac tatgggctat attcatagga
      301 atggctgttt ctcctcattt attagggaga gttataccag ccttaaaaaa gtttataggg
      361 gataatgaaa taaatgtatc tccatattta ttatctttga cactatatcc attagggata
      421 atgtttggaa ttaatgcagg acctaaagtt ggtatattac ttcaagcagg accagcactt
      481 ttattacaag aatttggtca tatgggtaca atgttgatag cattacctgt tggagtagca
      541 ttgggtcttg gaagaagtgc attaggtggt actttttcat tatgtcgtga cacagcacta
      601 ggaattattg gagataagta tggattaaat tcaccagaag gtataggaac actaggaact
      661 tacataagtg gtagtatatt tggaacatta ttattttcat ttttagcacc agtgggtgta
      721 cttttaggat ttcatccata tgctttagca atggcatcag gaatgggtag tggttcaatg
      781 atgggagctg caacagcatc attaatgaat acggttccac aaatgtcaga gcagatttta
      841 gcatattcag ctactagtgg attgattaca gctgttactg gaatatatat agaattattc
      901 ttatctttac cagtagccaa ctactattat agcaaagtag ctccagttat tgaaagattt
      961 agaggaagaa aaacaagaag aacaagtaat aaattgtaaa ggaggatata tatatggaaa
     1021 aaacttttaa gactattatg gagcagataa aaatagtatt gtttatatat attattattt
     1081 gctttggaca aatgatagca cttaagactc aggctcaaga tttgttgatt ggaagtgtat
     1141 ctggattttt gttggtgata attgctatta taataaagaa ttttgttaaa aaacctaatt
     1201 taccaggttt tgcatgggct acattagttg cattcgtttt atcgcttcca atatctccag
     1261 tgggggatat catagcaaca aatgtaggga aaatcaattt tatgtctaca gtaacaccat
     1321 tattagcttt tgcaggaatc tctgtaggta accaattaga tatattaaag actttgagtt
     1381 ggaagttagt agttatatca tttatagtta tgacatctac ttactttggt tctgctttta
     1441 tatctcaaac agttttaaga ttaaatggaa tgctttaagg aggtagtaaa tatgaatgaa
     1501 ttaaaagcaa aaattaaaga ggaaataaag ttattatctg aagagaaaaa gacatcattt
     1561 gaaaaagttt ctgactattt attttcaaat ccagaattag catttgagga gtataagtca
     1621 caaaaagcat tatgtgattt gttagaagaa aatgaattta atgtaactaa aggtgtagga
     1681 ggattagaaa catcttttga agctgtgtat tcaaatggaa ctaatgggaa aacagttgct
     1741 tttttagctg aatatgatgc acttccagga atgggacatg catgtgggca caatatcata
     1801 gggacaagtt ctgttggtgc aggtattgta ttaaaagaaa tcatgaaaaa acataatata
     1861 gaagggactg taaaggtttt tggaacacct gcagaagaaa gagtaggtgg aaaaataact
     1921 atgattaaag aaggtgtatt taataatgta gatgctgcat taattttaca tccaagtgat
     1981 gcttcaatgc ctgatgatat atcatttgct caagttaatt taaaatttga ttttactggt
     2041 aaggcatcac atgctgctgc ttttccttgg gaagggaaaa gtgccttaag tggagtaata
     2101 gcactattta acagtgtaaa tagcatgaga ttacatctta aagattatgc aagagtacat
     2161 ggaattataa ctgatggagg tagcatacat aatataattc ctgaaaaatc tacagctata
     2221 tttaatgtaa gagctttaag tatagaatat ttaaatgaga tttgtgaaat gcttaaaaat
     2281 tgtgctaaag gagctgcaat ttctactgga actaatgtag aaatagttca attagatgaa
     2341 atatacaaag agattaagaa tgattctgaa ttagtgaata tagttagaca aaattttgaa
     2401 gttttgggtg aagactatgt tgaaagagat ttaagtcaag gtataggttc aactgataca
     2461 ggaaatttaa ctcatgagat acctgcaata caagcatata taaaattaaa agaaaatact
     2521 gcaactcata cagatgaatt tgctgttgca gctggtggtg aagaaggcaa agtagcactt
     2581 ataaaagcaa ttaaggtgct agcaatgtgt ggagtagata ttttgtatag taaataatat
     2641 ataattttaa aatatattaa gaaaggaaat cagattagac atgatattaa aacaagtaat
     2701 agaggtttat gacattttag ataaagcaaa tgctaatggg gaagaagtaa aaacttactt
     2761 acaaggttat ggtgaagtag atgtaactgt aaaagaattg tcttcaagta aaggttcaac
     2821 agatttagta aagcttacaa ttcctggaaa aaatggaaaa ttaaaaggtg gagatgctcc
     2881 tactcttggt attttaggta gactaggagg gattggagca agaccagaag ttataggttt
     2941 tgtctcagat ggtgatggag cattggtagc aattgcagtg gctgctaaac ttttggatat
     3001 gcaacgaaaa ggggatatat tagatggtga tgttgtaatt tcaactcata tttgcccaga
     3061 tgctccaact aaagaacact atcctactcc ttttatggat tctccaattg atatgatgac
     3121 tatgaatgaa aatgaagttg atagtagttg tgatgcaata ctttctatag atactactaa
     3181 aggaaataga gtaatcaata ctagaggatt tgcaatatcg ccaactgtta aagaaggcta
     3241 catattaaaa actagtgata accttttaga tattatgcag actgtaacag ggaaatctcc
     3301 atttgtattc cctttgtcta ttcaagatat aacaccatat ggaaataatc tgtatcactt
     3361 aaatagtatt ttacaaccag cagtagctac agatgcacca atagtaggag tagcaattac
     3421 aacagagatg cctgttgctg gatgtgctac aggtgcaact aattttagtg atttagattc
     3481 agctgggaga tttgcaattg aagttgccaa gctatttggt agaaataagt gtgatttcta
     3541 tgataaagaa gagtgggaaa tgcttattaa gagatatggc aaattaaata gatttcaaac
     3601 atttggtata cagtaattag aaaaatatta agtttatata atttgtgata taactattta
     3661 agaagatata aacaattagg aataaaagta agaataaagc acttagttaa aaactttaaa
     3721 aataaaatat ttatttgttt atataaaaag gaggattctg gttattggga ttctcttttt
     3781 tatgaataca aataatatat tgtaattata aaaaaaggag cgtttcaaag tgattttttt
     3841 aatcaccttg agacaactcc ttatattttt atttaaattc tattttttat taagttgctg
     3901 gagttccacc accatcagga ggagtagtag agccaccacc attatttcca ccattatcag
     3961 gaggagtagt agagccacca ccattgtttc cgccattatc aggaggagta gtagagccac
     4021 caccattatt tccgccatta tcaggaggag tagtagagcc accaccattg tttccgccat
     4081 tatcaggagg tgtagaaccg ccaccattat cacctgtgtt attattgtta ttattatttt
     4141 cttctggttg gttattgtct ggtttttttg tatcttcaga tgatggttga gaacttactt
     4201 tagtaggagc agtaccttca ataaatgctg ctttagagcc agaagattgc tttcctccat
     4261 cctctaaatt aatttttgta aaatatacat ttttaggaac ttcaaattca gtaaccgtta
     4321 aattagcatg tattttttcc ataatttttt cccatagttt tgcagtacta gtacttccat
     4381 gtggtacttc tctacgtttt atagtgttac cagaatcatc ttttctacca gcatcatctc
     4441 caatataagt tgcaccaaca taataaggag tgtaaccgac aaaccaagca tcatatgcac
     4501 tatttgtagt acctgtttta cctgctacag gcattccctt tggaagtgca gcaacaccac
     4561 cagtaccttc agtaattaca ctttctaaca tatctgtaac gacataagct acttcaggtt
     4621 ctacaacttt atgttcctca ggagcatttt taactaataa ttgaccatca aaggtagtta
     4681 tagtagtaaa aatagaaggt tctacataaa taccgtgatt tgccaatgta ccataagcag
     4741 cagccatttg aagtggagta ataccattcg ccataccacc tagtgtcaat gatgggaatt
     4801 ttctatcact tgcttcagat gaacctgagt gattattctt taaagttgtt ataccaaagt
     4861 tctttaagta atccatcatt atatctacac attcatcata agagtctcca agcatttcag
     4921 cagttttgac agctatcgtg tttgaagatt tagcaagtgc tttacgtaaa ctcatagaac
     4981 catgaccagc agtagtagtt cttggattcc atttattatt ttcttcaaac ttatatcctc
     5041 ctcgagaatc acttagagca gttgcttgtg ttatttttag agtatcaata gctggcgtat
     5101 atattgctaa aggttttata gaagaacctg gctgatgagg gttagtagca cgatttaaag
     5161 tttttctacc acttatattt cttcctccag ctagtgctct tatttcacca tttttataat
     5221 ctaagattac cattgcagct tgcggttggc taactccatc aggaccgacc atatgtcctg
     5281 ggaaattgct atttctatca aattcttctt ctaaagcatc ttgcatttta gggtctatag
     5341 ttgtgtttac aattagacca ccattgtaga ataagttttg agcttcatca tttgtatgtc
     5401 cttgttccat taaagcattt ataacttcac tttcgataag gtcttctaca gatgaagaaa
     5461 ctttatctga tgattgaggc aattttattt ctattttagc tttgattgct tcatcatatt
     5521 cactttgttt tatatatttc aattctaaca ttttctttaa aacaatttct tgtctttttt
     5581 tagcttcagg attacttaca gcctttctaa caactaatgt acctgctttt aattgtctat
     5641 aagtatcatt agatataagc tcccaagatt ttattttttc aatcatatca aagtccactt
     5701 gagtagggtc atctaaatca tcatcagttg tgttaacaaa aaataataat ttattctcta
     5761 aatcctcctt agtttcattt ccctctaact tagaagtttt ataggctgaa aatcttgatg
     5821 gatttttagt tgaaccagca agaagtgctg actctgctag agtaaggtca gctgcagact
     5881 tatcaaaata accacgtgca ccagcctcag cacctgctag acctctacca acgaagaagt
     5941 tatttaaata agtttctagt attttatctt tacttactgt tttactcatc tcatgtgcat
     6001 agtaaatatc ctttatttta cgaggtatag tttgctgttc agttgttaac agcattttgg
     6061 atacctgcat aggaatagta ctaccacctt gcttagtacc tgtaagggtc tttaatactg
     6121 acctaaataa acctttaata tcgacaccct tatgctcata aaaacgctca tcttctattg
     6181 caattacagc atgttgaaga taaggggaaa tcttatctaa tggaacgggt gtctttttat
     6241 ttacacttgg agctgtagat aatgtttttc cattagcgta tttaatagta gttgtctggt
     6301 tcgtctgttt atctaaaaca gcttctgtaa ctggagttac atctcttagt gacgcaaata
     6361 ctagaccagc agtacctgct gtaccaacaa ctagtaaaac taaaaatact atcccaaata
     6421 ctcttagttt tttaaattta tcgctttttt tagatttttt cttgtttttt gttgaacgag
     6481 tcttgttagc tgaagttcta ttaattggtt tatttgtatt accagatgag ctaacttttt
     6541 ttctcctgat tttatttcca tttttatcat tatcgttatt cataagattt taacctcctt
     6601 acttaatgca atattataac acaaatagga gtagttttgt aattaataga aaaaatgaat
     6661 ttgctataat attctgtaca ttgtatatgt ataaacgtaa tatattaaaa agtctatatt
     6721 tttattttat ttatttttga caaatattat aaaaattatg ccaaagctac atataaataa
     6781 ataggagggg ataattttga atagaatagt agatagaaaa acaaaaaatg aatttagaaa
     6841 aatcatagct atttttttag taattctttt cttgtctgta tttataggca gttttatata
     6901 tatagataaa actttaagac cgacaataac agttttagca gagactaaag ctcttgaact
     6961 agcaaatagg tcgataaata aagctgttgc tgagatggta gaaggaaaaa taaattatga
     7021 agatttaatg gatatacaac ttgataataa tggtaaaata actatgatac aagcaaacac
     7081 tataatgatg aatgaaattg catctgcaat agctttagaa atacaagatg aattaaaaaa
     7141 ggataagaca gcaagttctt acatacctat aggaacagca ttaggaagtc ctatattagc
     7201 taaatacggt cctaaacttg aagtatctat tgaacctata ggaacagtat cagtaaactt
     7261 taaaacggaa tttgagtctt caggtataaa tcaaactaga catagaatat atttagaagc
     7321 tcaaacgcaa gttaaagtag taataccact tataacttca acaaaacaaa ttaaagctca
     7381 aatacctatt tgtgaaacta taatagtagg tgatgttcca gaaagttatg taaatattcc
     7441 agaaaaaaat ctaggaaatg ttttacctaa cactggaaag aataccaaca agtaatataa
     7501 agttgtatat ttttaaataa attcaaaata atagtttata aataaaattt ttggaaataa
     7561 tattaatata atattaataa aaatttatat tttatactat gtaaaatatc taatatattt
     7621 ggtctgtagg ttaaataaaa gtacctttag accaaatata ttattaataa cttaataaca
     7681 tcttaaaact tatttattta tattatttac aacatccatt taataaatac accataacct
     7741 acagctgggt cgttttcaat tgcatgtgac actgctccga ttattttatt atcttgaact
     7801 attggagtac cactcatacc ttgtacaata ccaccagttt gagctaaaag ctttttatct
     7861 gtaattttaa ttttaaaact tttactttct ggatgttttt ggttttctat atctataatt
     7921 tcaatattat attttttaca cttattaaag ttatcttgta aaattatttg tgcttttcct
     7981 aatttaactt catctaaaga tgctacttta tattttttta acttggaaat atcaaagtta
     8041 ttaatttgac ctcttattcc aaaatcagta ttttctgtaa agctaccaat aatattttct
     8101 cgttttgcat ttatacatcc aacactacct ttataagatt tttctatatt aaggtgctta
     8161 gttgtagaaa ttgaacctgt tttaataggt atttttcttg cattaccaac acttattgga
     8221 tgacctacag ctccaaactc tgatgtatct ggattgatat acgttaaagt agcaaatcca
     8281 gaaagtaaat tgttaaaact aattttttct aatatgtgtt tggtagtttt cattgttata
     8341 agttgtccac ctctatttaa taaaatagat acttgttttt cttctggtaa agcatttaag
     8401 gtattggcaa agtcactata tccatcaact tcagcattat ttacctttac tatagcatca
     8461 cccaatctaa atggagatgc ttctccagga tttctaacta tgatattttt taattctgcg
     8521 tcaatttgaa gtacgtttcc aattggtatt aaatattcag gttcattttt ttgtaaattt
     8581 tgtgcataaa ttaaattatt tgagaaaaaa tataataaaa ataaaaatgt taaaacaatt
     8641 gtaaattttg acttagctaa gttggttttt ttacttaaag tattattttt aaaatgaaaa
     8701 tttttaagtt gcataaaaca cctctttctt attaaattat tttaataata gaattgaagt
     8761 tttcttgctg cttctatatt atgttgtgca agaatcatgt tttgatgctt ggaaaactag
     8821 ctcaataaca ccttagtttt cataaaaata aataaaataa atagtgtata tgataaaata
     8881 aatacatatt catataaata aaaataaaca ataactgtct agcataagaa aggagcaaaa
     8941 aatgaaaaaa tatagattgt tagttacttg tataattgcc atgggaatca taattatagg
     9001 aacatcaaat ccagttagca atttttacaa gactacaaat ttgattagtt caggaaatat
     9061 cacaaatgaa aaagcagata aagatgaaaa ttcaaacaaa aataataaag ataaaaatga
     9121 agacaaatct aattctaaaa gtaataaaga taagtcatca gaggacaaat cgtcagataa
     9181 taaaaatgaa attcaaaaaa ataaaaaact tttaatttgt atagacccag ggcatcaagg
     9241 aaaaggagat agcaatttag aaccagtagc tccaggttct tcctctaaaa aagcgagagt
     9301 atcttcagga acagagggta ttgctacaaa aaagccagaa tatgttttaa acttagaagc
     9361 atctcttgtt ttaaagagta tattagaatc taaaggttat aatgtaataa tgactagaga
     9421 gacacatgat gtaaatataa gtaattcaga aagagcaata cttgctaatg ataaaaaagc
     9481 agatatggtt gtaagaatac atgcagatag tttaaataat tcttcaaaaa ctggagcatc
     9541 tatattgatt cctgaaaaag atggaaaata tacagctcct atatatgaag aaagtaataa
     9601 atgtgctgaa tttatcaaac agaatatgga acaatcaggt atacaaatca atggaatcgt
     9661 tcaaagaggt gatttgactg gattcaactg gtcaaaagta ccagctgtat tagtagaaat
     9721 gggatttatg agtaactata atgaagacca aatgatgtca aatccagact accaaagaaa
     9781 aatgatgcag tgtatagctg atggattgga tgcgtatttt aaataagata tgaatagaga
     9841 aaaagttagg ttggtattat tttcctaatt tttatctcta ttttttattg tacataacac
     9901 aaagaagaag tttattttag gttaaattgt cgtaaatata actaaaaata ttgggaatac
     9961 ttgatttaag tttaaaaaga atatattata gtatagaatg tgtaaaattt ttttgagagg
    10021 tgttgaaatg ctagtagtag aaaatgtgag tcatggattt ggtgcgagaa caatattaga
    10081 aaatgtatcc tttagactta gaaaaggtga acatattgca ctagttggtg ctaatggaga
    10141 aggaaaatcg agttttttaa atataataac aaagaagctt atgccagatg ctggaaatat
    10201 aaaatggtct tcaagagcta cagtaggtta cttagaccaa catacggtat taagcaaggg
    10261 aaaaactata agagaagtgc ttagagaggc ttttaagcat atgtttgact tagagcaaga
    10321 aatgattgct atgtatgaca aaatgggaga agctagtgat gatgaaatga gtaagcttct
    10381 tgaagaaaca gcagagatac aaactatact tgaaaatagt ggtttttata tgatagatgc
    10441 aaagatacaa gaagttgcaa atggtcttgg tcttggagaa attggtcttg ataaagatgt
    10501 aactgactta agtggagggc aaagaacaaa agtacttctt actaaacttc tacttgaaaa
    10561 tccaactatt cttatattgg atgagcctac aaactattta gacgaagagc atataacatg
    10621 gcttactaag taccttcaag agtatgagaa tagttttgtc ttagtttctc atgatattga
    10681 atttataaat aatacatgta atgtaatata tcatatggaa aatggagagt taaatagata
    10741 taaaggaaat tatgatgaat ttgtaagact taatgatata aagaaacgtc aagaagaaca
    10801 agcttatgat aaacaagttg aagaaagaaa aagactagag gattttgtag ctagaaataa
    10861 agctagagta gcaactagag gaatggctaa cagtagacaa aagcaacttg ataagatgga
    10921 aatattagaa agaccaaaag aaaaaataaa acctactttt gcttttaaag atgctagagc
    10981 agctagtaaa ataatttttg aaacagaaaa tttagtttta gggtatgatg aagcattgac
    11041 aaaaccactt aatttccatt tagaaagagg aaaaaagatt gctttaaaag gaatgaatgg
    11101 tattggaaaa tctactctat taaaaacttt acttggaata ataaaaccat ttgaaggaaa
    11161 tgttaaatta ggtgactatt tggaagttgg atattttgaa caagagagtt caagagaaaa
    11221 tagtaataca cctatggatg aagtttggag tgaatttcca gggcttacaa actttgaagt
    11281 tagacaggct cttgcaaaat gtggacttac aaatgaacat ataacgagtc aaatgagagt
    11341 attaagtggt ggagaagctg ctaaggttag actttgcaaa gtaatgttaa aaaatataaa
    11401 tttcttagta ttagatgagc ctacaaatca tttagatgtt gaggcaaaag atgaattgaa
    11461 gaaggctatc aaagagttta agggaacagt tcttttagta tgtcatgagc cagaatttta
    11521 ttcagaaatt gttgatgatg tatggaatat agaagatttt acaactaaaa tcgtgtagtg
    11581 atttaactta ttgctggggg gatttcatga aaaagtttct aaatgtactt ttttcagttt
    11641 gtattagtat tgttataata gtaggtgtta ttaattttac tgttggattt aaacaacttt
    11701 attattttga cattgattac ttaaatatat cagagttatc tggtttatct aaagatgata
    11761 taaaattgaa ctacgattat ctaattgatt ataacttaaa taagaatgtg tctgagttca
    11821 aattaccaac attaaaatct tctccacaag ggaagataca ttttgaagaa gtcaggaata
    11881 tatttcaaaa tataaataaa ttagctaaat tattattggt agtttcttta gtaggaatta
    11941 tattgagtgt aaaaaataaa aatataaaaa tcttgaaaac tacatcaata acacttataa
    12001 ttatgccatt attattaaca gttccaattt tattaaattt tgaaaagtta tttattattt
    12061 ttcataaatt attatttaga aatgattatt ggatatttaa tcccgattta gacccagtta
    12121 taaatatatt gccagaggaa tttttttttc actctggaat gatgatttta atattaattt
    12181 tgttagtaag tatagtactg tttgtaatgt ataaattatg taaatcttta tcaataaaaa
    12241 tatttcaatg ttaaatagag ggatattttt atttccaaaa taagataata aaaaaggatt
    12301 agattaaaag ttatacaata aaaatagctg tgcaataata tttagactat gaaagtagtt
    12361 aaaaaatatt tatgcacagc tatttataat agaatttact ttttactagt gattttaact
    12421 aaatcttgaa atttactcat aatgtaattg taattatctt ttttatgagg taacatacaa
    12481 ctactgcaat ctttgatacc tttatctgta tacttaaagt ttccaccaca gttttctcct
    12541 aaagcataca aaggacaata gcaaaataga cagttaaact cttctggttt attagtttta
    12601 tgacaaggga aaaattcgca atctttgtga ttaaaaaact tataattttc gcccatattt
    12661 tcctcctaat taaataaaat ttaaattttt tatactatat aagattttgg tgataatata
    12721 attatactaa ataattttat gaatagtcga taatatagta ttattaatca tagtattaat
    12781 ggtataatta gtcaacagaa aaataatgta aaaataatgt aagtgtattt tgtaattaga
    12841 atatgtttta taacaaaaaa tgaggtgatt aagtgaaaga aattcaagaa aaagcacttc
    12901 ttgtaggtct gaatttgact acaatagtga aaaaaaatga tgatatagac actaatgaat
    12961 ctatggaaga attaaaagaa ctaacaaaag ctgctggggc agaagttgta ggtagcttaa
    13021 ttcaaaataa acactctgta gatgcagctt attatatagg aaaaggtaaa gtagaagaaa
    13081 taagagccta tagtgactct ttagatgcta ctttagtaat ttttaatgat gaattatctg
    13141 gtgctcaaat cagaaatata gagaatgttg ttggaagaaa agtaatagat agaactactt
    13201 taattttaga tatatttgct caaagggcac ttagcaaaga aggtaaatta caagttgaat
    13261 tagcacaatt aaaatataga ttgccacgac tttatggaat gggtggtgag atgagtagaa
    13321 caggtgcagg gattggtact agaggtcctg gagaacaaaa actagaaata gataagagac
    13381 atatacttaa taaagcagca gatataagac gtgaacttaa agaagtaaaa aagaatagag
    13441 agacacagag agttaagcgt ttaaagtcaa atataccgat tgtagcactt gttgggtata
    13501 caaatgcagg aaaatctacc ctgttgaacg aattaataaa gacacacaag gattatgaac
    13561 aagaaaaaga agtttttgta aaagatatgc tttttgcaac tttagatgta actttgagaa
    13621 aggctctttt gccgaataaa aaagaatttt tagtagtaga tactgttgga tttgtaagta
    13681 aacttcccca tgatttagta gaagcgttta aggcaacatt ggaagaagta cagtatgcag
    13741 atttaatctt acatgtaatt gatgcaacaa atactagtta tgaattgcaa aaaagtacga
    13801 ctgaaggtgt attaaaagaa cttggtgtaa atgataaaaa acatatactt gtatataata
    13861 aagtggacaa gctagaattg gatatatatc ctaaaagtca agaagatatt gtgtatattt
    13921 ctgcaaaaca aggcataaat atggataagt tattaaatat gatagaaatt gctttaatgg
    13981 aaaatactta ttcagtaagt ttgatgttac cttatgagag aggtgatata tttagtagaa
    14041 taaaagataa atataatgtg gagaattttg agtatgggga aagtggtata acgctagatg
    14101 tagatttaga tgaagaagat tttaatatct atagagagta catactagaa aaataggttg
    14161 aatatggata taaactggga aaatatagat aatcttgaag attattttat aacctatctt
    14221 ctttataaag aatctaaaac agtatctcaa attagtaaaa ttagaaatat ttcaagtact
    14281 gaagcaagtg agcaacttat acaagcaaag ttaaaaataa aggaaatgca aaaagatgat
    14341 tttgaggcat ctaaggatat tttggataaa tttctagaac tagataaaat taagagactt
    14401 gattttatgg atagcttaga tgacgaaaaa atggtatact ttaagagaaa ggttttcaaa
    14461 agaatccttg tagaaaaaaa tgctgaagat ttaattgttt taatttgggc gacaggtgag
    14521 ctaaaagatg ataggttttt gaaattgctt catcagctca caaaccacag acactcagat
    14581 attagaagaa taacttattc agcaatcaga aagattgaat caccaagtag tagagaagta
    14641 ctgcaaaaag gtctctacga caaaaatgca cagactaggc agtactgtgc aaaagctctt
    14701 tcaaaattgg gagatgaaaa cagtttaaag attttacagc agttgaaaga caagaataag
    14761 aattttgaaa aagaatatgt gcttagagct tatgacgaag ccattaagtc attggaaaat
    14821 gcaaaaaatt tgaaaaagta attttttatt aaagcaattt gtttacttaa tgatgtattt
    14881 ctaaagttta acaaggtaat aaataaatta atgggagggg tttcaaatga tagtaagacg
    14941 ttgtgcaggt ggagtagtgt tttatgctaa taaagtactt attgtaaaaa atgatagggg
    15001 tgaatggaca cttccaaaag gaaagatact aggaggggga cttccatatg agagtgctgt
    15061 tcaaagagtt aaagtagaaa ctggcataga tgctaaaatg atagatgtgg ctggcgatac
    15121 tatgtatgaa tttttctcaa gaagtagaca acaagaagtt tgtaatgcaa taatgtggta
    15181 tgttatggaa gcttgtaaca ctgattgtgt acttgctcct gaatttcaag aaggtggatt
    15241 ttataaagta aaggatgctt tagaaatgtt atcacatcat aaagagcaag ctttagtaga
    15301 agtatcttat aaaaagttta aggaattaaa gaaattagtt tcagagggcg atgatgttcc
    15361 agcaaattaa ttgtgaattt agaggattct gaggaaggga tagccccttc cttttttaat
    15421 tgaagcagtt ttaagcgtaa attatatagt ctctttaaac tatgatttat caatttagag
    15481 atttagtagt ttatatttaa aatcagtaag atatatttaa aataaaaatt aaaaagtata
    15541 caattttatt atataattgt cttatgatgt tgattaaaga attattaaaa gtccatttat
    15601 ttttaaatag aataatttta taaatatata tttgatgtgt atattagaaa atgattaaaa
    15661 taggaggaaa tacaatgagc aattacagga cattacatga atttggaacg gatgaaataa
    15721 cgattgaaaa gtctgttttt attggatatg ctaagcctat aaagagtgaa gaagaagcat
    15781 tagagtttat aaatgaaata aagaaaaagc ataaagatgc aactcacaat gtgtgggctt
    15841 atactgtagg taaaaatatg aatatacaaa gatatagtga tgatggagaa ccacaaggaa
    15901 cagctgggat acctacatta gaggttataa aaaaagaaga tttgagagat gtagcggttg
    15961 ttgttactag gtattttgga ggaacaaaat taggtgcagg tggtctagtt agagcttata
    16021 caaaaggtgc taaactggga cttgaagcag gtaagataat ttataaggta atgtatcaag
    16081 aagttaaggt taagatagat tatactcaac ttggaaaagt gcaaaatgag ttaatgaatt
    16141 tggggtattt tataaaagat actgtatatg aggataatgt tgaaatagta gtatattcaa
    16201 gattagaaga tgtagagaaa ttatcagaaa aaatgattga tataacaagt ggaacaggaa
    16261 aaatagtttt aggagaggaa ttttatttgt ctgaacaaaa tggagaaatt ttaatataat
    16321 ctaaaaggtt attagaatag ttaatacctc aaatagccaa ttagtatttt taagtgaaaa
    16381 gccattaaat tgatttaatc taaatcaatt taatggcttt ttatcggatt ttgcacagaa
    16441 tccttttttt acttcaaata aaccgactaa aagtaaaaat atcccaaata ttttttttaa
    16501 tatatctgaa gacagataat tagctaatag tgaaccaaat acagctccta aaaccccaaa
    16561 cacagctata ggtataacca atttaaaaat tacattttta ttttttatat gtatagcaag
    16621 tgctataagt gtcataggga tagatgaaag taaatttacg ctttgtgcta ttttagtgtc
    16681 tatgccagca agtaaaacga gtgcaggtat aaggattgtt cctccaccca ttcccattcc
    16741 tccaataatt cctgcaaaaa atccaattag agcaaataac attaaaacac catccttaat
    16801 gctgcaatta tcataattat tccaaaagaa attctaagaa attttccagt aaccttattt
    16861 agtaatttag ctcctataat accaccaaga atacttccaa ctgcaacttt aattgttaag
    16921 ttaacatcat aagtaccttt agatacatat aaaacagaac ttgctgtcgt taagaatatt
    16981 attatagaaa gagcagttgc atgagattta tgttcttcta ccttaacgat gtcatttaat
    17041 attggaacta aaagagtacc tcctccagaa ccaaaaactc catttataaa tcctgtaaaa
    17101 actccaatta tcgtattttt taagttaaaa tcaaagtatt ttttctttaa atcatcagtt
    17161 tttttcataa aaatcacctt ctgaaatagt tttaccaaaa taggcaaatt atatttatat
    17221 aaaataagat taaatataat ctttcatgtt tgtttaattt tgcataaaaa aaaacatatt
    17281 aaaacaacat attaatatat ggtataatat attaaaacaa agtagcaaat ttgagaggag
    17341 ataacaatgt caaatataaa aatacaaata gacaaaactg tagaatggct tataaataag
    17401 gttaatgagg caaatgcaaa aggattaatt gttggtgtat ctggaggaat tgattcggca
    17461 gtagttgcta atttaattaa aaaggcattt ccagaaaact ctatgggagt tataatgagc
    17521 ataaaaagta atcctcaaga tagagaagat gcacttaagg ttatagaggg atgtgatata
    17581 gaataccttg atttagacct tatagaacca caaagtgcta tattagacat ggtagtagga
    17641 aatttaaaag ataaacattt atatagagaa gaatatttaa agatgacaga tgcaaactta
    17701 agagcaagag taagaatgag tacaatttat acaattgcta acaatctagg atatctggtt
    17761 gttggtacag acaatgctgc agaaatacac acaggatatt ttactaaatt tggtgatggt
    17821 ggagttgata ttttacctat tgcaaattta actaaaggag aggtgtatga atgggcaaaa
    17881 gagcttggtg ttcatgaaga tttaataaac aaagctcctt cagcaggtct ttgggaaggt
    17941 caaacagatg aagatgaaat gggaactact tacaatatga tagatgctgt gttagaaggt
    18001 agattggatg aagttcctaa aagagaccaa gagataattg aaagacttca tagattaagt
    18061 gaacacaaga gaaaaacgcc tgcacaacct cctaagttct aatttactat ttataagatt
    18121 tgtgataaaa tttacaagtg atgaaaatta gaataattaa ttgtacgtat taaggagtgg
    18181 aaatatggga cgtataggta atataattaa tagaaaaggt aaacaagatg ctcaaagagc
    18241 aaagatattt actaaacatg ctagagcaat agcagttgca gctaaagaag gtggagcaga
    18301 cccagagtac aatgcagcgt taaaaacagc tatagaaaaa gctaaggcag ataatatgcc
    18361 aaatgataat atagatagag ctatcgctaa aggtgctgga gcaggtgctg gagaagacta
    18421 tgaaactata gtttatgaag gatatggacc tggtggagtt gctgttatag tagaaacttt
    18481 aactgacaac aaaaacagaa ctgctggtaa cgtaagatac tattttgaca aaaacggagg
    18541 aaacttagga actagtggat gtgtgtcttt tatgtttgat aaaaaaggac aaatattagt
    18601 tggtttagga gatggagttt cagaagaaga attgatggat gttgcattag aagcaggagc
    18661 tgaagatttc ataacagaag aagatggtta tgaaataata actacaccag aagatttttc
    18721 tagtgttcgt gatgagttaa aagctaaagg atatgagttt atatctgctg atgtgaagat
    18781 gatacctcaa actacaactg tattgactga agaatctcat ttaaaaatga tgaataaatt
    18841 agttgatatg cttgaagaag atgatgatgt tcaagatata tatcataact gggaagtaac
    18901 tgaataatgc tttaaagtca gtaatcatat atgatataat tcataacgat tatgatataa
    18961 ataattatag tttttgttga agatgtatga ataaatatta tatacattta atcagtatat
    19021 acctcttttt atagaaaata taggactttc tataaaaaga ggtatatttt ttgtgtaatt
    19081 aaattaacta aatcggaaga aaaatattta agtgcttttt aaagtttgtt taaaagaaaa
    19141 ctacatagga ataaatataa taaatttata gaagttattt gtggaaagtg gaaaaatacc
    19201 tatgtacata ataatagtat tatatatttt aagtaaaaaa ttttaggtga ttattttgaa
    19261 agggataaaa tatatgttga aaagaattat tatatttgac ttatattata aaaataaaaa
    19321 tagaatctaa acttagaaat aag
//
LOCUS       DAEORZ010000072        19200 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEORZ010000072 DAEORZ010000000
VERSION     DAEORZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.072"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(43..1422)
                     /locus_tag="KRH39_002240"
     CDS             complement(43..1422)
                     /locus_tag="KRH39_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG8512139.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(1415..2095)
                     /locus_tag="KRH39_002241"
     CDS             complement(1415..2095)
                     /locus_tag="KRH39_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG8512140.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(2486..3565)
                     /locus_tag="KRH39_002242"
     CDS             complement(2486..3565)
                     /locus_tag="KRH39_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8512141.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      3853..3968
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4143..5513
                     /locus_tag="KRH39_002243"
     CDS             4143..5513
                     /locus_tag="KRH39_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8512142.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(5778..6389)
                     /locus_tag="KRH39_002244"
     CDS             complement(5778..6389)
                     /locus_tag="KRH39_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBG8512143.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(6379..8742)
                     /gene="lon"
                     /locus_tag="KRH39_002245"
     CDS             complement(6379..8742)
                     /gene="lon"
                     /locus_tag="KRH39_002245"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBG8512144.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIAPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            8922..9434
                     /locus_tag="KRH39_002246"
     CDS             8922..9434
                     /locus_tag="KRH39_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG8512145.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            9422..9949
                     /locus_tag="KRH39_002247"
     CDS             9422..9949
                     /locus_tag="KRH39_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG8512146.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(10020..11270)
                     /gene="clpX"
                     /locus_tag="KRH39_002248"
     CDS             complement(10020..11270)
                     /gene="clpX"
                     /locus_tag="KRH39_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBG8512147.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(11289..11873)
                     /gene="clpP"
                     /locus_tag="KRH39_002249"
     CDS             complement(11289..11873)
                     /gene="clpP"
                     /locus_tag="KRH39_002249"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG8512148.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(12023..13309)
                     /locus_tag="KRH39_002250"
     CDS             complement(12023..13309)
                     /locus_tag="KRH39_002250"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBG8512149.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(13503..13973)
                     /locus_tag="KRH39_002251"
     CDS             complement(13503..13973)
                     /locus_tag="KRH39_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG8512150.1"
                     /translation="MKIGILSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(13973..15322)
                     /gene="rph"
                     /locus_tag="KRH39_002252"
     CDS             complement(13973..15322)
                     /gene="rph"
                     /locus_tag="KRH39_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBG8512151.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNVHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(15482..17506)
                     /gene="ligA"
                     /locus_tag="KRH39_002253"
     CDS             complement(15482..17506)
                     /gene="ligA"
                     /locus_tag="KRH39_002253"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBG8512152.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(17692..18630)
                     /locus_tag="KRH39_002254"
     CDS             complement(17692..18630)
                     /locus_tag="KRH39_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBG8512153.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(18732..19154)
                     /locus_tag="KRH39_002255"
     CDS             complement(18732..19154)
                     /locus_tag="KRH39_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBG8512154.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
BASE COUNT         5580 a         3532 c         2091 g         7997 t
ORIGIN      
        1 ataaagacaa ctgaccaaac gaagtttaac tatacaattc agttaaatca tttcatttac
       61 tactaaacac tcttctaatg tcgttatttt ttcatttatt agtcttttac aactatcttc
      121 aaatgttatc attccattat tttgtgccac atctttaata gtactagaat cctccatatt
      181 tcttatacat gtctttatat catcattaat ttctaagatt tcataaattg ctgttcttcc
      241 aaagtatcct ttatcattac atttttcaca ccctacagct accttagtat ttacatctcc
      301 actaaaatta tcttttatca taatctcatg gctacaatga ttgcagactt ttctaaccaa
      361 cttttgagat ataacaccta taagagaggc atttaaaaga tatggtggta tttgcatttc
      421 tagtaatcta gctatggaag atactgcatc attagtatgt aaagtactta taactaaatg
      481 acctgttata gctgctctta ctgctatctt agctgtttct gcatctctta tttctccgac
      541 cattattata tctgggtctt gtcttaatat cgctctaaga cctacatcaa agctcaaccc
      601 tacttttgaa tttacttgaa tctgacttat gccatccatc ttatactcta caggattttc
      661 tatagtcata atatttttat ttattccttt taaatcattt aataaggaat atactgttgt
      721 agtctttcca ctacctgtag gacctgtcac taataatatt cctgctcttt tatttattat
      781 acgatttata gattttatag cttcctctga aaaaccaagt tctaatttgt cttttaaaaa
      841 agtatctcta ttaagtatcc tcaaaactat tttttcacca tagacagttg gtattgatga
      901 aactctaatg tcaattaaat ttccacctaa ctttatatcc accctaccat cctgaggaag
      961 ccttttttct gttatatcca ttgatgcctt taattttata accgtagaca atgatggata
     1021 gacatcaact aaaaactttg atacttcctt tagttctcca tctaccctca ttctcactat
     1081 taaatgttct ttaaatggtt ctatatgtat atcacttgcg ttttctttta tagctttttc
     1141 taaaagattt tcaaataatt tttttgcata atcctcatca agttcaaagc tattataata
     1201 tttttcaatc ttttcactta taagttcttt tttctccttc ttcaatataa tttctttacc
     1261 tgtagctaat ctcaagtcct gtagagcata aatatcctca tcttctattt ctacaactaa
     1321 attatcatct tgaaactcaa ctggaaatac attatatttt tttgcaagtt tttctggtat
     1381 taacattggc aaactgcttt cctctaatat aatcttaatc accctttata tactttaatt
     1441 tattaggtat attcattttt ttcatagaga tattttcttc agtttttatg cttattttca
     1501 ttggataatt ggtttgtatg ctatctaaat atttactacc tttatctaca taattagata
     1561 gtttctcctt atctccaatg cactttattt catatggttg tgctacagga actccattta
     1621 tatttatatg gcttcctgca agtacaactt ctgaatattg attaattctt tgaccattta
     1681 tttctatgta ttttcctccg tttactttta attcatttat aacttttaca agaatcttat
     1741 tatagtctat actattagct atattccctg ttttttcatc tatagcatct atacttaata
     1801 ttatcccact accctttaca tctgaatatg atagactatc tctaagtgtt tctatttctg
     1861 atatttcttc agaatttttt tgattttgtt tcattgaatt aatttcttta tctaacttgt
     1921 ctttctcttt ggttaattct tttatagatt ttcttgtcat ttttatttct tttcttattg
     1981 acttgtcttt gcttgtataa actgtttctt cattgctata atctctaaat atggttccaa
     2041 caagtatacc taaaatagta cttgctagta ttataaacac atattgtttt ttcatactgt
     2101 attgctcctt acaaatttat ttttatatga taattttctt taattaaata ttaaacattt
     2161 acatatgctt attatatcaa agttatatat taaaattcat ctttagtata taatatcata
     2221 cattgaactc ctatcactaa tttaccaata tcaagtatgc atttttagat gttcttaaaa
     2281 ttatataaac tttcaataaa cattgttgtg atattataaa aaaacataaa ttaggaaagg
     2341 aaataataat tatgaaaaac aaataatggc tttggagatt attaatatca ttttttagca
     2401 aaaaaagtaa aatggcatag cctcaaagct acaccaattt actaaaaatt atattattac
     2461 taacgttaat tttaaacata tgcgtttata agttttcctc atattttaaa attcttttaa
     2521 ttaacttatc tcttaatttc cctacttcat caaatctcat attaggacta tagtaacttt
     2581 ctataatatc atgtttagac ttggctctct taagatttag tactgcccaa caaattaaat
     2641 catctaaaac tttttcatca aatttaatat cttcttcata tttaagtagt ttttctctat
     2701 ttaaaaactg atttaaatca attgtatctt cttctttaaa agtcgtactt atagttatgc
     2761 ctatatctaa atcagttaat agtacagttt caattttctc tggaataagt ggataatgat
     2821 aaacttctac atttaaacct ttttcaatag ctttcttata tactttgttt aataatgtag
     2881 ttttcccagt tcctatttct ccatctaaat aatagacttt ctttacatct tttaaaatgt
     2941 tttctgtata atctaaacac cctacaggag ttatagctga accaaataaa tgcttttctc
     3001 tttttagatt tcctgtgctt ttaactccat caaataacct ttttataaat tcttctgtga
     3061 ccaaatttac ttttccataa tccatacaat cagaatattt ttcttctatg tctctaaaga
     3121 ttggttctgc tgattttaaa aacttaaatg ctcttttaaa tgaattacta atatctttat
     3181 cataaactga tatttcttgt ttattctttt ctaattcttc tgtattccag taatcaccta
     3241 aattaattat ctcatccaaa gctccaggta gctttgggtc catagtatgt ggggcagttg
     3301 catccataag taccacgcct aatttcttta taactaaacc atccaacgaa cttgggtcag
     3361 aaggacaatg atataaatca atatcatatc ctctatgcaa aaactcttta taaatttttt
     3421 tcataagtga ggattttcct actccaggtc caccctttaa acaaaaaact ctttttacat
     3481 tttcaggaat tatatagtca aaaaaagaat atgagccttg tgatgtattt ccacccggga
     3541 atgccttctt tacctttcct tccatattag cgcctcctca taatttacaa tatctcttaa
     3601 ttaagatatt catattataa gaattttatt acagctttct gattttgata aatttaaaac
     3661 ttaccatcta atactaataa caggttaatt ttatctaatt attatagatt ctcatactgt
     3721 tccttattct atctataaat acaatttaag tgtccatatt gaaatatttg tattgtaata
     3781 cagctggata ttacttaaat ccaattgttt ccattataat tttatgttaa aataatatta
     3841 caaaatacat ctgtttttct tcataaacgg gtgaaattcc ctatcggcgg taaaagcccg
     3901 cgagccttat ggcataattt ggtcatattc caaagccgac agtaaaatct ggatggtaga
     3961 agaaaatagt atatgagtac ctttatgtaa ttttacatga gtaatctata caaatccttc
     4021 aactaccgta tttattcatg aaattagaca cattcaaggt acctaatata caggtgcttt
     4081 ttttgttgtt tattttacaa ttatatcgta cttataaaat ctattaagat tggagtgtta
     4141 tcatgaaaca aaaatggata gtattgatta tcatctgtat tggtgtattt atgtctactc
     4201 ttgatggaag tatactaaat atcgcaaatc ctacaattgc tgctgacttt aaaattaaca
     4261 tgtcacaaat tcagtgggtt gtaacagctt atatgcttgt tgtaacagct actatgttat
     4321 ttttcggaaa acttggcgat aaagtaggtt caaatcgtct atacacatta ggatttttta
     4381 tatttacaat aggttctttt ctgtgtagta tgtcaaacaa tttatcaact ttaatttctt
     4441 caagaatctt tcaggcagtt ggagcaagta ttttaatggc tactgggctt ggtatagttt
     4501 ccaatgcatt tccagcaaat gaaaaaggaa aagctatagg aatcactgga gctgtagttg
     4561 gtataggaaa tatgagtggt cctgtaattg gaggtataat tttagaacat tttggatggc
     4621 cttctatttt tattatcaat atacctattg gtattatagc agtctttctt ggcattaaat
     4681 ttctgcctaa acctgttttg gatgaacaaa acaagagctt tgatattcca ggtcttctac
     4741 ttttcgcatc ttgcactacg cttatattac ttgcaatgaa tgagaaaggt aatacaagat
     4801 tgtatttagg tataacagca cttataatct tcttattgtt ggcattaaga gaagtaaaat
     4861 ttgaacaaag ctttattgac ctacctctat ttaaaaatag aaactttact gtaggcaata
     4921 tcattggagt agcttgttat tttcctcaaa tggcagtatc ttttctactt ccattttatt
     4981 tagaacaatt aaaaaattta tctcctatga tggctggtta tgtaatgact gtacatccac
     5041 ttattatggt acttatagca cctattgcag gttcactttc tgataaacac ggtgcaaaaa
     5101 acattctaac agcatctttt tcatttatga ccatctcact agttggaatg gcacttttaa
     5161 aagcagattc tccattgtat cttttaattg tatgtttagt tatttttgga cttgggctag
     5221 gagcatttag ttcaccaaac aatagtagta ttttagcaga tgttcctcct caaaaacagg
     5281 gatacggagg cagttttctt gcaactattc gtaatttatc atttgctctt ggaacagcat
     5341 tttttagtag tttttttgca caaagtctta catacaatca aaagtttaaa agtcacacat
     5401 cagcctatgt tatagctagc aatcagtctt attggattgc agcatcagta tgttttatag
     5461 ggcttatact aactgttttc tttatgagaa aaacagataa atctatttct taaatgctat
     5521 ttattgctga ctacataagt caataatttt ttagttgaac taatttatac taattttccc
     5581 ataccttact aaaatttatt agtatttagc aaaattactg atatttattg tttataaaca
     5641 ataaggatgc caaaaaaagt tcattaaaac tattttaagg catcctatat tttgctttaa
     5701 tattaatata attaaaacta actattatta catttattat tcaaataaat cttatgtcaa
     5761 tatttaacac tttaaattta atctactgtt atttcatatc catgttgatt gtattgattt
     5821 acaatctctt cccataattc ttcaacacca gtctttttta gtgaagaaac aggaattact
     5881 ttttcactac tttccatttg caattttttt cttattatac ttatgtgttt ttgatattgt
     5941 cctctagata ttttatctgc cttagttgca ataactacac agttatatcc aaagtgcttt
     6001 atccattcat acataagctt atcatctgct gtaggctcat gtctaatatc tacaagtagg
     6061 aatatagaac ataactcttg tctactttct agatatcttt ccattatacc tccccacttt
     6121 tctttttcag acttagctat ctttgcatat ccatagcctg gaaggtctac aaaataaaat
     6181 tcattattta ttagataaaa atttatagtt ctagtttttc ctggtgtttg actggttcta
     6241 gcgaagtttc ttctgttaag caatgtattt attattgatg attttccaac atttgaccta
     6301 ccagccaatg ctatctctgg gattccttct gctggatact gtgatttatt cacagcactc
     6361 atagttatct ctgaacttct aattttcatt attttcacca ctctttaata aagcttgttc
     6421 taatacttca tccatatgtt ctacaagcac aaactccatt ttttctttta cattttctgg
     6481 tatttcatct aaatcagctt cacattcttt aggaattaat acttttgtta taccagctct
     6541 atgagctgct aataactttt ctttaactcc tcctactgct aagactctac ctcttaaagt
     6601 tatctctcct gtcattgcta tatttccagg cacaggtctt ttagtaagtg ctgagattac
     6661 tgcaagagcc atagttatac cagctgatgg tccatctttt ggaacagcac cttctggaat
     6721 gtgtatatga atatcattcg tcttataaaa ttcaggggct atatcaaact tgtccactat
     6781 tgaccttata taagagatac ctgtcttcgc agattccttc ataacatctc ctaattttcc
     6841 agttaataca atttctcctt tacctttaag tacgtttact tctacttcaa gtgtaactcc
     6901 accaacttct gtccatgcaa gaccatttac taatcctatt tgagggttaa cttctgcaag
     6961 ttgatactta aacatgtctt ttccaagata tgtctctagg tcagacttat ttataacaac
     7021 ctcttcaaga gttgggtctt caacgtattt tttggcaacc tttctacaaa tcttaccaat
     7081 agttctttct agtgttctta caccagcttc tcttgtataa tggttgatta tacttcttaa
     7141 agtttcatca tctattttta taaagttttc ttttaaagca tgttctttaa tttgttttgg
     7201 aagtaggtat ttttttgcta tatttaattt ttcttcttct atatatccag atacttctat
     7261 tacttccatt ctatctaata atggtcttgg tatattactt agactatttg ctgttgtaac
     7321 aaatagtatt tttgacaaat caaaaggtat ttctaagtaa tggtccacaa aatccttatt
     7381 ttgctctggg tctaatactt ctagcattgc tgaagctggg tcacctttat aatctgctgc
     7441 catcttatct atctcatcaa ataagaatac aggatttttt gtttgggctt cttttacccc
     7501 attaattatt cttcctggaa ttgaacctac ataagttctt ctatgacctc ttatttctgc
     7561 ttcatctcta actccaccca aagatattct tacaaatttt ctatttaatg ctctggcgat
     7621 tgattttact atagatgtct ttcctgttcc tggaggacca actagacata ttataggccc
     7681 tttaagtgac ttagcaagag ttcttatagc tagatactct agtattctct ccttaacttt
     7741 ttctaatcca taatggtctt catctaatat atcttttgcc ttagttatat ctaatttatc
     7801 ttttgtctct ttattccatg gcaatgagaa tatagtatct aaatagtttc tacttacaga
     7861 tacatctgga gacatagaag atatttttga aaatttatct atctcttttt ctattttctc
     7921 tttagttgtc ttaggtgctt ttattttttt aagcttttct ctatactcat ctgcttcaga
     7981 gtttatatct tcttcttcac ctaattcttt ctgtatagct tttaattgtt ctctaagata
     8041 atattctttt tgaaccttat tcatctgttt ttttactctt aaagttattt tcttttctat
     8101 ttttaatata tctatttcct ctaataatat agaataaata agttcaagtc tttttctaat
     8161 atcaaattct tctaatattt cttgtttttg gcttgatttt agatatatgt ttgcagctat
     8221 cgtatctata aatctatcta catcttctat atcagctaaa gatattaata tttctggtga
     8281 taccctattt ccaatattta tatactcttc aaaggcatca aatacatttc ttacaaatgc
     8341 ttcaatttca acttctgttt cactatccaa attatcacta tcaaatacta tttcttctat
     8401 tactgctctg aaatacccat cttcttgttc tattttcttt actctacccc ttgatacacc
     8461 ttcaaccaaa acacgaactg tatctcctgg taattttatc atttgcttaa ctttacagat
     8521 tgttcccaca tgataaaaat cctcttctcc tggctcatct acctctgctt ctttctgaga
     8581 agttaaaaat ataagttctt catccatcat agcttggtct aaagctttaa gagatatctc
     8641 tctacctata tcaaaattta atatcatata tggaaatata gctaatcctc ttagaggaat
     8701 taatggtaat tcatgatcta ttttagtata attttgttcc atcaacatta ctccttttct
     8761 agtctaattg ctttgctttt aatattatat atttgaacaa gttaattttc aacattaatg
     8821 ataaattctt agaatactat catactatat tatcttttta ttatgatata atattaatat
     8881 tagtatactt agatgttatt tatcttttgg aggtttctat tatgttgaaa ttgtttttaa
     8941 cctttttaaa aattggtgcg tttacatttg gtggtggata cgctatggtt cctcttatac
     9001 aagatgaact tgtaaacaaa caaaaacttt tagagcagga agaatttatt gattatttat
     9061 ccatagctca aagttatcct ggagtactgg cagttaatat atctgtatta ttaggatata
     9121 aactttatgg tataccagga gttcttatat gtaccttagg ctctgcttta ccatcatttt
     9181 gtatagtctt agtcttatcg tatctatact ttaaaaattc atcatctaaa attttagatg
     9241 gattttttaa aggagttgcg cctgttgtaa tagctcttat attgtattct tttacgagca
     9301 tgttcaagaa actaccaaag tctaaaacta acatactact attaattata gcagttttat
     9361 ctgttggtgt attcaatata agccctattt atttaataat tggaggaggt gtatatagtc
     9421 tatgtcagaa ataatactac tcttcttaat attccttaaa ataggggcat ttagttttgg
     9481 tggcggttat gccatgctac catttatcca gcaagaattt atatctaaat atcactttat
     9541 aactaatcaa gagtttttgg atttacttgc tatatcacaa tctacaccag gtcctgtagc
     9601 aataaatagt gctacatttg taggatttaa gactgctggc ttctttggtt ctctagctgc
     9661 aactattggt gttacaattt ttgcattaat aggtctaact attatatcaa agttattttc
     9721 taaatttaaa aataataaaa aggtagaaaa cttattgaca gttcttagac caattactct
     9781 aggattcata ttatcagcag ctttttcatc tgccaaagaa gttaattggg atttatatgg
     9841 aataattgca tttatagcat cattttatct tttatataaa ggaaaaattg gttccattgc
     9901 aattgtattc gtctttggta ttctaggtat attactcaca tttttataat aatgatacct
     9961 attttatatt tagcatatta acataaaaaa aggagctttt aaagctcctt ttcatcatct
    10021 tatgcacttt cctcttgatc cttaagtact atgataggat ttactgagtc ttcgttcaca
    10081 gatttctctg ttactataac cttctttata ttatctctag atggtacttc aaacatagtt
    10141 tccatcatta cgctttcaac tatacttcta agtcctctag ctcctgtatt tctttctatg
    10201 gcctttttag cgattgcacg aagagcacct tcttcaaatt ctagctcaac atcatctaac
    10261 tccaataact ttttatactg ttttactaga gcatttttag gttcctgtaa tatctgcatt
    10321 aatgcatctt catccaacaa ctctaaagta gctaaaactg gtattcttcc tataaactct
    10381 ggtattatac catattttaa taaatcctct ggaagtactt tttcatatag cttccctaaa
    10441 tctaattctt ttttactttc tattttagct ccaaaaccta gtgttttatc tccaccacgt
    10501 ttttgaatta tcttctctaa tccatcaaaa gctccaccta atataaataa tacattagta
    10561 gtatctatct ttaaaaattc ttgatgagga tgttttcttc ctccttgtgg tggaacattt
    10621 gcaacagtac cttctaatat ttttagaagt gcttgttgaa caccttcacc actaacatct
    10681 ctagttatag atgggttttc agattttctt gcaatcttat ctatctcatc tatatatatt
    10741 atacctcttt cagctttttc tatatcaaaa tcagcagctt gaataagttt taaaagtata
    10801 ttttcaacgt cttctccaac atatccagct tcagtcaatg atgtagcatc tgccattgca
    10861 aaaggtacat ttagagttct tgctagagtt tgagcaagta atgtctttcc agaacctgta
    10921 ggtcctaata aaagtatatt actcttttga atttctatat ccttactact agatttctta
    10981 ctgtatattc tcttataatg attataaacc gcaactgata aagctttttt agctttttct
    11041 tgacctatta cataatcatt tagaatctcc atcatttcct ttggttttgg taaagatgta
    11101 gtatcctcat ctatagtatc ttctatctct tcttgaataa tttcgtcaca aagttcaaca
    11161 cactcgtcac aaatatatac atttggacct gcaattaatc ttcttacttg atcttgattt
    11221 ttaccgcaga atgaacactt taattgtcgt ttttcttcat atttagacat attaacacct
    11281 ctctctatct acggtctctt tgtaaatact tcatctatta aaccatattc tttggcttct
    11341 agtgcactca tgaagttatc acgttcagta tccattttaa ttttttctaa tggctgacca
    11401 gttctttcag ataatatctc atttaaagtt tcttttattt ttaaaattct ctttgcatga
    11461 atttctatat ctgttgcttg accttgagct ccacctaatg gttgatgtat cattatttca
    11521 ctatttggta gtgctagtct ttttcctttt gctcctgctg ccaacaagaa tgctcccata
    11581 gaagcagcca tacctataca tatagtactt acatctggtt tgatgtattg catagtatca
    11641 tatatagcca ttccagaagt tatacttcct cctggagaat ttatatataa atgtatatct
    11701 ttgtctgggt cttcagcttc taaaaataat agctgtgcta ctataagccc tgctgtggca
    11761 tcgttaactt gatctcctaa gaagattatt ctgtctttta atagtctaga aaaaatatca
    11821 taagatcttt ctcctcttcc tgtttgttca acaactacag gtactaatgc catggtttca
    11881 tacccccttt attctatata atttacagta gcttttttta agctactgta aattattata
    11941 cgcatttaac taaattatat ttgcacatca ttatatacta aacatttcat aacgtcaact
    12001 atattagtta gcgtttattt tattaagcta ttgttgcatt atcaactaat aaatctattg
    12061 tttttcttat ttttatttga ccttttatat cttctttgtc ggcatctctt aaagaagctt
    12121 ctattttttc tacttccata ttgtaagcag aagccatttt ttctaactca gccttaaatt
    12181 cttcttctgt tgcttcaatt ccttcagctt tagttattgc ttctaatact aaagaagact
    12241 taactaattt tttagcatct tcttttctct cttctcttaa ttcttccata gttctaccag
    12301 tcatctctag taattgttgt aatccaaatc cttgatattg taattggtag ttaagttcat
    12361 ttaacatatt atctatttga tgttgtatca taacttctgg tatttctatt tctgtatttt
    12421 cagcaacttt ttctactact gaatttctag tttctgcatc tgctctattt ttagcttctt
    12481 cttctaactt agctcttaca tctgctttta attcatctaa agaatcaaat tcactagtat
    12541 ctttagcaaa ttcatcatca agtgcagata attcttttac ttttacatca tttattttta
    12601 cattaaatac aactggtttt ccagctaaat cttgtgagtg atattcttct gggaaagtaa
    12661 cattaacttc tacttcttca ccagcttttt tacctactaa ttgttcttca aatcctggta
    12721 taaatgtgtt tgaacctata actaagttat aattttcacc tttaccacca tcaaaagcta
    12781 caccattttc aaaaccttcg aagtctatta tagctgtatc tccatcttct aaagctttgt
    12841 cttctacact tactaatctg gcattttttt caaccatttc ttgtagttta gcatcaacat
    12901 tttcgtcact tacagtattc tcaaccttag ctatttctat acccttgtag tttcctaatt
    12961 caaactctgg tttaacttct acattaagaa ccataaccaa cccattgtct ttacttattt
    13021 cttctatatc taaatctgga ttgtctattg ggtctatgtc taattccttg atagcttctg
    13081 gataaacttc tgggaataat atttctatag catcattgta gaatatacct tttccgtatt
    13141 gagtctctat aacaattctt ggagctttac cttttctaaa tccaggtata ttgtatttat
    13201 ttctgctttt attataagcc ttatttactg ctgcttcaaa tttattgtta tctactgtta
    13261 tttttaaagt aactttatta ccctcttttt taattagttc tgctttcatc cttctaatcc
    13321 tcctagtgat atttatgtaa tacccgataa cggtattata attcattcta tttataattc
    13381 aaccacttaa ttatatcaca taagtttttt tatttacaag tgttccatta atatcgttgt
    13441 acttatttga taaaatttaa ttttttgagc gttttttgcc atttttttag ataaatacac
    13501 ctttatatgc gtatttcttc aatttttatg tcatcttctt ctatatccat aatgacaaaa
    13561 cttttatacg atactcctct tggaagtgaa gtgcttccag gatttatgta aagtacacca
    13621 tctttaattt ctctaaatgg ggtatgggta tggccaaata taactatatc agcatcaact
    13681 tcagttgcct ttttttctaa catattagtt ccatatttta caccatactt atctccatgg
    13741 cataagaata tggttttatt tgccacttca aagacaactt cttcctcaac agcatcaaag
    13801 tcacaattac ctttaactgc aataatacct acattagtca tgctatgaat atatcttgaa
    13861 tcagtaaagt tatctcctgc atgaattatt aggtcacatt ctttcaaata tggtattgtt
    13921 ttttccatat ttttaatcat catatgtgtg tcgcttaata tgcctatttt cattacttca
    13981 ctaccctttt tataaactct tgtttcataa gttttagtgc gtttgctcta tgactgattg
    14041 aattttttat aacactaggt atttcaccaa aagtcttatc atatcctttt actataaata
    14101 agctatcata tccaaaacca tttttacctt tttcttcaaa tcctatcatt ccttcacaaa
    14161 ttcctcttac tacaaattct tttccatcag gaaaaactac tgctattgca gagacgaatc
    14221 ttgcatttct tttgctcatt ggtacatttt gcatagcttt taacagtttt tttctatttt
    14281 cttcatctgt tgcatgttct cctgcatatc tagctgaata aacaccaggt tttttgccta
    14341 aagcatctac ttctaagcca gaatcatctg atattgctat aagtttagtc ttttttgcta
    14401 ttgttctagc ttttatcaac gcattatgct caaatgtttt tccatcttct actatctcta
    14461 tacctgctaa atcaacatct tttaaagagt acactttata ttcaaaatct tttaagattt
    14521 cacctatttc ttctagctta tgaacattat tagtagcaat cactacttca ttaccataat
    14581 ccatacccaa tatctcatct gatatttcac ctaaagcttc tctttgaagc cttattaatt
    14641 ctttattccc tttttcacct aactctaaca ataaatttaa atctgctctc gaaaaaggtt
    14701 tctcttctcc tgtcccctgt acttctacaa actcacactt atcagtcatt attatattca
    14761 tatctacctg tgctttagaa tcttcttcat aacataaatc tagcatgtgt attccattta
    14821 caataccaac actaattgca gatacaaaat ttgttattgg catctctttt attgtctttg
    14881 ctttatataa tttatatatt gcttctgcaa ctgctacaaa tgcccctgtt atagatgcag
    14941 ttctagttcc accatctgcc tgaatcacat cacaatctac ccaaatagtt ctttctccta
    15001 atttattcaa gtctacaaca gaccttattg ctcttcctat taacctctgt atttcttgtg
    15061 ttcttccatc aatttttccc ctagatgcat ctcttatctt tctttgttgt gttgctcttg
    15121 gtagcataga atattcagca tttatccatc cagtaccact atttcttaaa aaaggaggaa
    15181 ctttttcttc cactgatgca gtgcatataa cctttgtctc tcccatttct ataagaacag
    15241 aaccttctgc atactttgta taatctctag ttatctttac aggtcttatc tgttcattac
    15301 ttctatcata acttctggtc attttttata tcctttctca tattttttat tttttataaa
    15361 tttattttta taatttttaa ttttgtcaaa tattaattat tgtcattttt cgtaatctta
    15421 ttagcttact gaactattct atcattaatt atagtagttt tctactaaat taagcgactt
    15481 tttaactctc taatacagct tttacttcat ctttcgatga taactttaat aattctaaaa
    15541 atctctcttc atctataact ttaattccta aatcatttgc tttagtaagt ttagaaccag
    15601 cttcttcacc tgccaaaaca aatgttgtcg atttactaac actagatgta gcttttccac
    15661 ctcttttttc tatcatatct tttgcatcat ttctttttaa tgtaggaagt gtaccagtca
    15721 atacgatttt catcccttca aatatttttt ctactttttc acctgtatta tccaatgatt
    15781 ttgtatttac tcctacattt tttaacttgt ttataacgtt catatttttg tcttccttaa
    15841 aaaactcgac aacgctgttt gccattatac ttccaaattc ctctaattct tctagttgct
    15901 cactagttgc agaaattact tcatctaaat ccttaaagtt atatgcaagt atcttagctg
    15961 ctttaactcc tataaattta atacctaatc catttataag tctccataaa tcattttctt
    16021 ttgatttttc aattgaattt attaaattgt cagcagattt ttcgcccatt ctctctaggt
    16081 cgactacatc ttcctttttt atataatata aatcagatac atcttgaata attttttcat
    16141 ttaatagcag tgtaattata gattctccca atccatctat attcattgca tcccttgaca
    16201 caaagtgtat aattcccctt cttatttggg ctgggcaaga catattgata cattttacag
    16261 cagcctctcc ttctaatctt attgttggct ctgaacaaac tggacacttt tctggcattt
    16321 taaaatctat ttcatctcca gtacgttctt cttttataac ttctaaaact tgaggtatta
    16381 tgtcccctgc tttttgaact aatactgtat catttatttt tatatctttt tcccttatat
    16441 aatcttcatt gtgaagtgta gctctactta cagtagtacc tgcaagtctg actggttcaa
    16501 gaattgctga aggtgttatt gtaccagttc tcccaacttc tactattata tctactattt
    16561 tagttttctt cttttctgct gggaatttat atgcaattgc ccatctagga cttttggctg
    16621 tatatcccat ttcgtctctt tgttttatat tgtttacttt tattaccata ccatctattt
    16681 cgtagtctaa attacccctg ttatctgtcc agtattcaat atgctcaata acttcatcta
    16741 tacttttaca aactttataa ctctgactta ctgaaaatcc tagtttttct aagtactcta
    16801 gtgattcact gtgactttct aaatcgtatt cttctatatt ttccatatta aatacaaata
    16861 tatctaatgg tctctttgct gttagttttg ggtctaattg tctcaatgaa ccagctgcaa
    16921 gattccttgg attagcaaat aatggctgtt cttgttcttc ttgttgttca tttacttttc
    16981 taaaattatc ttttgatata tatacttctc ctctaactat tagttctttt ttatcattta
    17041 tcttaagagg tatgcttttt actgtcatta agttttgaga aatatcttct cctacaactc
    17101 catctcctct agttgctcct ttttcaaact ctccattgtt ataagttaga cctacagaaa
    17161 gaccatctat tttaaattca acaacatatt caacattttc tcccacatat tcttgaactc
    17221 tcttatcaaa atctctcaaa tcttgctctg aaaaagcatt tgataaactc aacataggag
    17281 ttttatgtac aatttgattg aacttatcta atggcttacc tcctacccta tttgttggag
    17341 aatcaattct ctttagttct ggatgttctt cttctaaaga aattaactcc ttcattaact
    17401 tatcgtattc ataatctgat atttctgggc tatcttgatt ataatacctt tcattatggt
    17461 agtttattgt ctctataagt ttattaattt catcttttac gctcactttt tgcacctctt
    17521 catattttta aaattaaatt attttgaaat ttccctataa ttaattatct cctatttata
    17581 tattttttag tatatcactt ttttggcttt aaatttatta caaaaaaaac tcttattagt
    17641 atctttaata cgactaataa gagtttttat ttttatcaaa attttttatt tttaatagcc
    17701 tcttgatatg tctacaacat tatctacagg tttattctcc attatatagt gtttcaaatt
    17761 attataaacc atattaaatg ttctctcttt gtttttatct gacacccaag aattatgagg
    17821 agttactatg acattttcgc actcccacaa cttattgtcc ttgtttaaag gttcactttc
    17881 aaatacgtct aatgctaccc ctctaaattt ctctacacat tcttctaaat ctttctgatt
    17941 aactgtattt cctctaccta catttataaa tacagaacca tctttcataa gtttaaacat
    18001 atctttgttt actattccat ctgtactttt tgtagaaggc attgctacaa ctactatatc
    18061 acattcttca aatacaatat tcatatcctc agtagcaaaa cacttatcaa agtatttttt
    18121 actacttcca tcagtattga caccccatat ttctacacca aaagctttta atctttttgc
    18181 tgcttctgaa gatatagtac ctgttcctaa aaatccaact ctttgtccat acaattctga
    18241 tacagaaaaa tccatcttcc attctttatt ttgttgttgt ttaaaaaatt ttgcactatt
    18301 tttatatatt tctaaaatat acaatacaat actttcacca ataggtacac tatatccgcc
    18361 tctattatta gctatcttaa tatttctctt caatacctct tcttttggaa gttggtctat
    18421 acctacacta gttgtctgta tatattgtaa atttttcatc ttagaaatat ctaatctctc
    18481 aaatggattg tatgtaacta gtacatctgc tgtatttaca tcctcattat tttcaatact
    18541 atcttcagag taatgtatga catcatagcc taattctctt attttatcaa atttttcttt
    18601 tccataattt attgtaaata aaactttcat ctcaaatctc ctccatctac ccacatttaa
    18661 aattttatat tctaaaatat aatatgatac tcaatgtaca ttagttaaaa ttactataag
    18721 atatcttaaa tttacttatg ttcaattgtt tttcctaaag acatagcatt acgttcttga
    18781 actaaatttt ctattaaatt attcaatgtc caatcaatag atgtctctgt taccatagct
    18841 gtaaaaggtc tatttattct cacttttttt agcccttcta aaaatgcatt tacttgtgtg
    18901 cttggagtgt tattaaatat tacagactta ctcttaatcc cattttcaca ttcatttgat
    18961 atatttccat ctaaaatgtc tcttattata ttctctccat ttttatcact taagatgata
    19021 tggtctctta ttccagcaaa gttacttacg tttttaatta atgataattc ttttttatta
    19081 aagtttacaa gcataataca cttcctgttg tgtattttat ctaggttatt attatttatt
    19141 ttttcaaatg acattttatc catcctttca tttatttata tactttaatt taccccatac
//
LOCUS       DAEORZ010000073        19137 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEORZ010000073 DAEORZ010000000
VERSION     DAEORZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19137
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.073"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            228..1421
                     /gene="nifV"
                     /locus_tag="KRH39_002256"
     CDS             228..1421
                     /gene="nifV"
                     /locus_tag="KRH39_002256"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBG8512155.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1446..3371
                     /locus_tag="KRH39_002257"
     CDS             1446..3371
                     /locus_tag="KRH39_002257"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBG8512156.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3393..4388
                     /locus_tag="KRH39_002258"
     CDS             3393..4388
                     /locus_tag="KRH39_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBG8512157.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4714..5187
                     /locus_tag="KRH39_002259"
     CDS             4714..5187
                     /locus_tag="KRH39_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8512158.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5221..5715
                     /locus_tag="KRH39_002260"
     CDS             5221..5715
                     /locus_tag="KRH39_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8512159.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5794..6333
                     /locus_tag="KRH39_002261"
     CDS             5794..6333
                     /locus_tag="KRH39_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG8512160.1"
                     /translation="MKGMIYMIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSK
                     GLRTWEFIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAA
                     SYIQLQAHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEE
                     KKAYSDSDLDYKKVHFNHF"
     gene            6522..6833
                     /locus_tag="KRH39_002262"
     CDS             6522..6833
                     /locus_tag="KRH39_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG8512161.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6861..7547
                     /locus_tag="KRH39_002263"
     CDS             6861..7547
                     /locus_tag="KRH39_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG8512162.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEAIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            7779..8663
                     /locus_tag="KRH39_002264"
     CDS             7779..8663
                     /locus_tag="KRH39_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG8512163.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            8762..9937
                     /locus_tag="KRH39_002265"
     CDS             8762..9937
                     /locus_tag="KRH39_002265"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBG8512164.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            10065..10919
                     /locus_tag="KRH39_002266"
     CDS             10065..10919
                     /locus_tag="KRH39_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512165.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELICIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            10963..12198
                     /locus_tag="KRH39_002267"
     CDS             10963..12198
                     /locus_tag="KRH39_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBG8512166.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            12299..13240
                     /locus_tag="KRH39_002268"
     CDS             12299..13240
                     /locus_tag="KRH39_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBG8512167.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(13459..13812)
                     /locus_tag="KRH39_002269"
     CDS             complement(13459..13812)
                     /locus_tag="KRH39_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG8512168.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            14108..15019
                     /locus_tag="KRH39_002270"
     CDS             14108..15019
                     /locus_tag="KRH39_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBG8512169.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIIPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEILEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFTSDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            15279..15713
                     /locus_tag="KRH39_002271"
     CDS             15279..15713
                     /locus_tag="KRH39_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512170.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            15736..17271
                     /locus_tag="KRH39_002272"
     CDS             15736..17271
                     /locus_tag="KRH39_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512171.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            17355..18722
                     /locus_tag="KRH39_002273"
     CDS             17355..18722
                     /locus_tag="KRH39_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8512172.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            18757..18981
                     /locus_tag="KRH39_002274"
     CDS             18757..18981
                     /locus_tag="KRH39_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBG8512173.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
BASE COUNT         7643 a         1814 c         3604 g         6076 t
ORIGIN      
        1 cataataact ttcattttta agaagttttc acaaaaaaga aaaaatatag tgcaataaat
       61 gatacaaagt aatataatag attacaagat atgaaaagct aaaaaaataa attgcaaatg
      121 aaattaaaat gaataaaaaa taaaaaaata atcataagtt agaggtatca atgaaaaata
      181 agacttaatg tttttaaaat taaaagtata ctaagggggg cattaatatg tgcgttatat
      241 caaaagatag agcaaaggaa ataaaaatag tagatactac tttaagagat ggagagcaaa
      301 cagctggggt tgtatttgca aatagagaaa aaataatgat tgctgaaatg ttaagtgacc
      361 taggtgttga ccaaattgaa gttggtatac ctactatggg tggagatgaa aaaaatgtaa
      421 taaagcacat atgtagtaga aatttaaaat cagatataat ggcttggaat agagcagtaa
      481 taaaagatgt tgaagaatct atttcttgtg gtgttgatgc agtagctata tcaatatcag
      541 tatctgacat tcatatagaa aataaattga gaacttctag aggctgggtt ttagaaaata
      601 tggctaaaac agttgaattt gcaaagaaaa atggattata tgtttcagta aatggagaag
      661 atgcttcaag agcagatata gacttcttaa ctgaatttat aaatgttgga aaacaagcag
      721 gggcagatag atttagatac tgtgatacag taggagttat gaatcctttt tctataaaga
      781 atgcaataga aactctttat gaaagaacta attttgatat agaaatgcat acacataatg
      841 actttgggat ggcaactgca aatgcacttg caggaatagc agcaggagca aattatgttg
      901 gtgttacagt aaatggatta ggtgaaagag caggaaatgc agcattagaa gaagttttaa
      961 tggcacttaa gtgtgtttat aaatgtgatt taaataatat agatactaga aaatttagag
     1021 gaatatgtga gtatgtagca caggcatcag gaagaatact tcctacatgg aaaccagtag
     1081 ttggggataa tatgtttata catgaatctg gtatacatgc agatggagct ttaaaagacc
     1141 cacataacta tgaaccattt gacccaagtg aagtaaatct tgaaagaaaa atagtaatag
     1201 gtaaacattc aggaagagct gcagttgtaa ataagttgag tgaatatgaa atgtatattt
     1261 ctccagaaaa cgctaccaag ctattaaatg ctataagagc tacatctata agattaaaaa
     1321 gaagtttaat ggataaagaa attcttcaat tatattgtga tatactagca catgagaaag
     1381 gcacaacaga agaagaagca gttagaggtt catatattta agatataatt gtttaggagg
     1441 aaaatatggg agataatata gtttataaaa taattaaaaa acatatagta gatggagaag
     1501 ctgttgctgg aagttcaatt ggtataaaaa tagaccaaac tttaacacaa gattctactg
     1561 gaacaatgac atatcttcaa ctagaagcta tgggaataga taaagtaaag actaaaagaa
     1621 gtgtggcatt tgtagaccat aatatgttac aacaaggatt tgaaaatgca gacgaccata
     1681 agtatataca aacagtggca gataaatatg gagtttattt ctctaagcca ggaaatggta
     1741 tttgtcatca agtattttta gaaagatttt caacaccagg agatacactt ttaggttctg
     1801 atagccacac acctactgct ggaggtgtgg gcatgatggc tattggagct ggtgggttag
     1861 atgttgcttt agccatggca ggaggagcat actatataaa agcaccaaaa gtctgtaagg
     1921 tgaatctagt tggaaaatta aataatatgg tatcatcaaa agatataata ttagaagtgt
     1981 taagaaaaca aactgtaaaa ggtggagtag gtaaagttta tgaatatggt ggagaaggtg
     2041 tcaaaagcct atctgttcca caaagagcta caataacaaa tatgggggct gaacttggag
     2101 caactacttc aatcttccca agtgatgaaa aaacactaga attttttaag agtcaaggaa
     2161 gagaagacgc atgggtagag ctaaaaccag atgcagatgc agtttatgat gaagagatta
     2221 ctataaactt ggatgaatta aaaccattag ctgcaaaacc tcatagccca gacaatgtag
     2281 atgaagttga aaatataggc aagattaaaa tagaccaagt ggcaatagga agttgtacaa
     2341 attcttctta tgaagactta atgaaagtag cacaaatcct gaaaggaaat aaagtacata
     2401 aggatgtgag tttggtaata gcaccaggtt caagacaagt tatggagatg attgctagaa
     2461 atggggcttt ggcagatata ataagtgctg gagctagaat actagaaaat tcttgtggtc
     2521 catgtattgg tatgggtcaa tctccaggta ctgactctgt gtctttaaga actttcaata
     2581 gaaatttcta tggtagaagt gggactttgt cagcacaagt atatttagta agtccagaag
     2641 tggcagcagt aagtgcaatc aagggagtgc ttacagaccc aagagaattt gatattaaat
     2701 ttacaaattt agatgtaaat gagtttttga tagatgatag catgataatt aaacctgctg
     2761 atgtaggaag tgatgtagaa gtagttagag gacctaatat aaaacctttc cctcttaata
     2821 cagagctaag tcagtctata ggtggaaaag ttatattaaa gacagaagat aatataacta
     2881 ctgaccatat aatgccatca aatgctaagt tacttccttt tagaagtaat ataccgtatc
     2941 ttgcaaacta ttgtttcaat actgtagata cagaatttcc tcagagagca aaagacaata
     3001 atggtggatt tatagttggt ggagataatt atggtcaagg ttcaagtaga gaacatgcag
     3061 ctttagcacc attatatcta ggtgttaaag gggtaatagt taagagcttt gcaagaatcc
     3121 acaaggctaa cttgataaat agtggtataa ttccaatgga gttttgtgat gagaaagatt
     3181 atgaaaatat atctctttta gataatctgg agataccaaa tattttagac aatctaggta
     3241 gtggaatttt agaagttaag aatacaacta aaggaacttc ttttaaggtc aaagtagaat
     3301 tgtcagcaaa agaagttgat gtattaaaag ctggtggaaa attaaactac actaaaaatc
     3361 aagctaatta atatgaaaag agggaatatt gaatgtataa agtgacactt ataccaggag
     3421 atggaatagg accagaagtt gcgaaagcta tgaaaaaggt agtagaagct actggagtag
     3481 aaatagaatg ggaagaagtt aatgcaggag aggcagttat tgaagagtat ggtactccac
     3541 ttcctgaata tattatagat agtataaaaa agaacaaaat agctataaaa ggcccaatta
     3601 ctactcctgt aggtaaagga tttaggagtg taaatgtaac tttaagacag gcattagatt
     3661 tgtatgtcaa tttaagacct attaaaagtt ttaaggggat aaaatcaaga tatgaagatg
     3721 ttgatttagt ggttgtaaga gaaaatacag aagatttata tgctggaata gaacataaaa
     3781 taggtgatta tgcagctgaa agtataaaga taataacaag aagtgcaagt gaaagaatcg
     3841 ttgattttgc atgtaactat gttaaagata ataagagaaa aaaagttaca gctatacata
     3901 aggcaaatat aatgaaaatg tctgatggat tgtttttaga tgtgtttaga gaagttgcat
     3961 caaaacatgg tgtagaatat gatgatttga ttgtagatgc agcagctatg aacttagttt
     4021 taaatccaga aaattatgat gtaatggtaa tgccaaatct ttatggagat atactatcag
     4081 atttaggtgc tgggcttgtt ggaggtttag gtataattcc aagtgcaaat attggtaaag
     4141 attgtgcgat atttgaagct gttcatggaa gtgctccaca aatagctggt caaaataagg
     4201 ctaatccaac agcacttatt caatcatcag taatgatgct tagatactta ggtgaatatg
     4261 aaaatgcaca aaaaatagaa acagctttag aaaaagtatt tcttgaagga agtaaattga
     4321 ctgtagattt gggtggctca gcttctacta cagagtttgc ggatgaggtg tgtaagtata
     4381 ttgtatagat tttatttagg gaaaaagtaa aatatagatt gaaggaaaga ggctttctca
     4441 aaatagagat aagccttttt tattggaatg ataaaatata tttgatttta tacaaaagag
     4501 agttcctcat aaacgaaaaa gttaattttg ggatatccat tttaatttta aaattaattg
     4561 attaatatgc aaagaaagat taaataaatt tattattttt aattgaactt tcaaccaaaa
     4621 tatggtaaaa tttaaaaaag ttgaactttc aactaaaaat gaatatattg attatgtgaa
     4681 attctataaa acagaaacta tttgacttta gccatggtag tttcagtgga ggtgtgtttg
     4741 attaagactt tagatagtaa tatattaaga gaagtaggta ctttatctag agcagtaaat
     4801 tcaataaatg atattaagta caaggaatta aagttacaaa aaggacaatt tacatttttg
     4861 acaagaatct gtgaaaatcc tggtataaat cttgtggaat tatcaaatat gttaaaggta
     4921 gacaaggcta caacaaccaa ggctatacaa aaattaataa aggcaggata tgtagataaa
     4981 aagcaggata agtttgataa aagaggatat aatttaactc caactgataa atctttggaa
     5041 gtttatgagt tgataattga agaagagaat agaagtatag agatatgttt tgataatttt
     5101 acagatgaag aaaaacaagt ggtaactaag ttattagaaa aaatgagcaa aaacgtagaa
     5161 aatgaatggt ttaaagtaaa gagatagtaa ataaatacat taacttaagg agatattttt
     5221 atgattagaa aagcaaatat gaatgattta gaaagcatta tgaaaattat aaaatctaca
     5281 gtggaagaaa tgaagactta taataataca caatgggatg aaaactatcc attagaaaaa
     5341 gactttgttt cagatattaa gaaacaagat ttatatattt atgaagtgga tggagaagta
     5401 gcagggttta tatgtcttaa ttatgaagag ccagaagaat atttaggctt aaattggtca
     5461 tctaataaaa aagcaatggt aatacataga atggctgtaa atccaaactt tagaaagaca
     5521 ggaatagcat ctaagttagt tgattttgct gaaaaattag ctgtagaaaa caatgtatca
     5581 tacttaaaga gcgatacata ttcaataaac tcaaaaatga atttattact tacaaagtgt
     5641 ggttttataa aaatcggaga aatgagtttt ttaggaaagg agaaatcttt ctactgttat
     5701 gataaaatat tataaagagc atttataata caaggggtaa ataatagatt ttcagcatta
     5761 gtaagggcat aatataacct agtatatgct attatgaaag ggatgattta tatgattgga
     5821 tttctaaaga aaagaagaag tattagaaaa tacaaggatg ttgaagtaga aaaagaaaaa
     5881 ttagataaaa tattaaaagc tgctctttta gctccatcat caaaggggct tagaacatgg
     5941 gaatttatag tggtagatga taaagaaaag ctaattaatt tatctcaatg tagaactaaa
     6001 ggtggtggat ttttcttaaa aaatgcacct ttagctattg taattattgc agataaagaa
     6061 aaaaatgatg tttggataga ggatgcaagt atagctgctt catacataca acttcaagct
     6121 catgaactag gtttaggttc ttgttggata caagtaagaa atagaatgca tgatgataat
     6181 atagaagctg ataagtacat tagagaagaa ttaaaagtac ctagcaagta cagtgtagaa
     6241 tgtataattt ctataggata tcctgatgaa gaaaagaaag catacagtga ctctgattta
     6301 gactataaaa aggttcattt taaccacttt tagtatataa tatgttgtat taagacatta
     6361 tatttttttc tattgttaat atatacttaa aattgtaaaa atatatctga attttactaa
     6421 cttatctagg ggttatgtac aaggatttta aagataaata tagaaattaa tgtttataat
     6481 cgatacataa cttgagtaac aaaatttaag gaggttaaag tatgggtata tttaaaacaa
     6541 aaatggatga ggactggaaa gtaaactaca ttaaagagtt taatgaaatg agagattctt
     6601 atgagagtaa acttcaaaaa aaacagtttg aggtagatag tttgaaatca gaattggata
     6661 gactaagaag ctataaaaat agccttaagc caaaagaaaa acaaattact gatgatgata
     6721 taaataatat aaagagttta agaagagatg gcttaagcta taaagaaatt tcaaatcaaa
     6781 caagttggag caaagcaact gtaagtaggg tattaaatgg actttacgat tagcatatac
     6841 atggggtgat ttaaattggg atgaagtata gttacaaaaa atgcataata gattctattt
     6901 tattgatgtt tatagttcaa atattaagga tgattttgaa ttatgtactt ctaagtcaat
     6961 ttgaatttac tcttgaaaat tttaatatta taaacttaat ttcttttaca ttagttggtt
     7021 tatcgttaat tttattttta aaagataatt ctttgtataa caaggttaga aatagaaaaa
     7081 tcacagaagc ttttgaagaa aataaaaaca atatacttat tgaaaagtgt aagctgatac
     7141 tttttgttgt ggttttatct ttagcaataa ttgtaactta ttgtactaag ggatatgtac
     7201 tttttaatgt aactatgatg actttgtctg tattgatagt tccaatattt gaagagttat
     7261 tttttagaga atatatatgg aactatctaa gtaattttat aaagtcaaag agtaaaatta
     7321 tctgtataac atctatacta tctggaattt ataatatagg atacatagat gttataagaa
     7381 attatgtaat attgtataac aattcgagct acacctttga agctattatt tcaaaaatta
     7441 tgataggtac tgtttttggt attgtacttg gattagttaa gtataggttt agagatgtag
     7501 ggttttgtat attattgagg agcttatttg ctatatttat taggtagata gtataatcgg
     7561 tttataaggt tgttttaaat ttaattttaa atcaacctta ttttatttgt gttttataaa
     7621 gcacttatat attaataact aataaattta ataaaaatta tttgaatatt tttaaaatat
     7681 ataaatatgt attgacatat aaatattatg aattacaatt tatataaact aataaaaatt
     7741 aaataatatt aatcaactta ttgaatggag gaataaaaat gaaatttgtg actttttgta
     7801 gtagcaatga agaaaaaata ggtgtattta attcagaaac aaatagtata tatgaaatta
     7861 atagtttagg attaagtaaa ttatatactg atatgaatga ttttattgaa aacgtatcta
     7921 caggagattt agaaaaaata aaaaacaatt catttgaaaa cgcaaagtgt tataagttag
     7981 aagaagttaa attatgttcc ccaattgtaa gacctaaaaa agatattata tgtttaggtc
     8041 taaactataa agaccatgtc aatgaaatcc cagatggagt tataaaaaac gtagttatgc
     8101 cagattatcc aatttatttt tctaagagag cagacaaaat aattggagtg gatgataaga
     8161 taagcttaca tggagatttg gtagagaagt tggactatga atcagaactt gcagtaatca
     8221 taggtaaaga aggtataaat atatcaaaag aagatgctta tgaatatata tttggatata
     8281 caatagtaaa tgatataagt gaaagagcac ttcaagataa acatgttcaa tggtttagag
     8341 gaaaaagctt agacacacat acatctatgg gaccttgtat agtacataaa gaagaatttg
     8401 aacatccttt aaaattagat ataagtagtg tagtaaatgg agaagtaaga caagattcta
     8461 atacagagta ctttatattt gatataccta ctataataaa tgatttatca agaggaatga
     8521 ctctaaagcc aggagatata atttcaactg gaactccagc aggggttgct atggggatga
     8581 atcctcaagt atatttaaag catggcgata tagttgaatg taaagtcgaa ggaataggag
     8641 ttttaaaaaa tatagttgat taatggataa atatgccata taattttatt agtacgaata
     8701 atttaattaa tataggatta atcaaactta tgattaattt attatagaga gggatgagta
     8761 tatggcaaga gaactagaat taaagtatgg ctgtaatcca aatcaaaaac catcaaagat
     8821 ttatatgaag aatggagaac ttcctataga ggtattaaat ggaaaacctg gatacataaa
     8881 ttttttagat gcatttaata gttggcaact tgtgaaggaa ttaaaagaag ctacaggtct
     8941 tccagcagca acttcattta aacatgtaag tccagcagga gcagcagtag gggtaccttt
     9001 atcagatact ttaaagcaaa tatattttgt tgatgattta gagctttctc cattagcttg
     9061 tgcttatgca atggcaagag gagcagatag aatgtcttct tatggtgatt ttatagcact
     9121 ttcagatgta tgtgataagg agactgcaac tattatagca agagaggttt ctgatggtat
     9181 aatagctcca ggatatacag atgaagccct tgaaatatta aaaggaaagc gtaaaggcaa
     9241 ctataatatt gtaaaaatag atgaaaatta tactcctgaa cctattgaaa caaaggatgt
     9301 ttatggaatt acttttgaac aaggtagaaa tgaaatatta ataaatgaag acctattaaa
     9361 agatattcca actgataata aaatttttac agatagtgca aaacgtgatt taattatagc
     9421 tcttattaca ttaaaatata cacaatcaaa ttctgtttgt tatgctaaag atggacaagt
     9481 aataggggta ggtgcaggtc aacaatcacg tattcattgt actagacttg caggtaacaa
     9541 agctgatact tggtatcttc gtcaacatcc taaagtatta aacttaaaat ttaaaaagga
     9601 tataggtcgt ccagatagag ataacactat tgatgtatat ctttctgatg attatatgga
     9661 tgtgcttgct gatggcatat ggcaaaactt ttttgaagaa aaaccagagc ctctaactgg
     9721 agaaggaaaa agagcatggc ttaaaacttt aacaggagta gcacttggtt cagatgcatt
     9781 tttcccattt ggagataata tagaacgtgc aaaaagaagt ggggtaagtt ttatagcaca
     9841 accaggtggt tctattcgtg atgataatgt tatattaact tgtaataagt ataacatagt
     9901 catggcattt actaaaaata gattattcca tcattaaaat actgaaagta taaagataat
     9961 atagattaag taaaaatcta caacaagttt tgttgtagat ttttttattt gctgtataat
    10021 tttaaataga actttatttt tagatattca atttggaggt tgatatggct ttaaatataa
    10081 gaaactatag cctattatgt aatcaaggaa cagctaaatt taatgaagat gtagtcggta
    10141 taaatccatt tggcgcatgg gttttagatg gtgctacagg acttaatgga aaaaatttag
    10201 tgtcaactga aagtgacgca aggtggtatg ttcagtggtg gaataggtat ttatataaaa
    10261 atataaataa agaagaatca ttaaagagta ttattaatac tggaataata gaagtaaaaa
    10321 aagagtatga actaagatta aatgggttta aggttgaaaa attagattta ccatctagtt
    10381 ctatagttgt tgtaaaattt catgaaaata aagtagagta ttttttatta ggagattgtg
    10441 ctttgtttat taaaaatgga gattccaaag ttataaaaga taggagtatt tgtaaatttg
    10501 ataatactgt atttgatgaa atgagtaaac tttctaactt ggaagagatg gcttttgatg
    10561 agattagaac tagtgttatg gatacaatag taagcaatag attgaagaaa aatacaaaag
    10621 agggctattg gatattggat tttgatgagg aagctgtgga aaattctata tatggataca
    10681 ttgaaataaa aaaagatttt caattgatgc taacgagtga tggttttaca agtgcatgtg
    10741 atagatataa tctaataaaa gaagaagaat tgatatgtat agcagaaaaa tttggtatag
    10801 gatatataca taataaagta agggattttg aagataatga ctataaagcg gttaaagtgc
    10861 caagatttaa agtaaaagat gattcatctt gtttatattt agatatatat aatgattaga
    10921 gtgtataaaa ctatttaatt ttagaaagga ggcaagtgaa aagtgaaaat tcttcatata
    10981 ataacacaaa aaccaaatag tacaggtagt ggtatatatt tgtctggtat gataaaaggg
    11041 ttcgaaaaaa tcggtcataa gcaagctgta attgcaggta tagatgtaaa tgatgatgta
    11101 aattgttttc caagtgaagt aagtttttat ccagtaaagt ataattgtgg ggagttaaat
    11161 tttcctgttg taggtatgag tgattctatg ccatatgaaa gtacaagata taaagattta
    11221 aatatagaca tgataaatag attgaaatat caatttaaag taaatataga taaggctatg
    11281 aatgatttta aaccagaact tataatatgt catcatttgt accttttaac tgcatttgtt
    11341 agggaaatgg taaaagatat aaaggttatg tctatttgtc atggaacttg tttaagacag
    11401 ttaaatacca ttgatttaga gaaagaatac ataatagcaa atataaggaa attagattta
    11461 atatttgctc ttcatgaaaa tcagaaatat gatattataa agacttttgg agtaagtgaa
    11521 agtaaagtag ttgtaattgg tagtggttat aatgatgata tattttataa taaaaattat
    11581 aaaataaagg atgataaaat aaaaatagta tttgcaggta agatatgtaa atcaaaggga
    11641 ctgattccat ttataaaagc tatatctaaa ttaaaatata gtaaagattt aatagaagtc
    11701 aattttgcag gtactggaag cgatatcgaa tcatataatg agatagttaa actggcaagt
    11761 aagtctccat ttaaaatgaa tttcctagga aagttagaac agagagattt agctgagcta
    11821 ttcaacaggt cacaaatatt tgtgttacca tcattttatg agggattacc agttgtagtt
    11881 ttagaagctt tatcttgtgg cacagatgta atcactactg atattcttgg tgttaaagag
    11941 tggattggta gtgagattaa taattctgga aagatagaat atgtttctct accttttatg
    12001 gagaaagaag gtattccaaa agatgaagag ttatatgatt ttgaaaataa tttgtataat
    12061 gcaatagatt ctaagataca atctttattg aataactcaa ataagaaaac atctgtagat
    12121 atgtcaaaaa aaacgtggga tggcttagca tatagaataa atgaagttat tttaatggat
    12181 gaattgtgtt tagtctaaaa ttatttaaat tataaaaaag tgtaactatt tttacactat
    12241 ttttaaatga ttttatatta tactaatact aaatatattt gttttaggga ggtaatttat
    12301 gaggattagt aaaaaggtat tggcgttagg tatttcagca ttactgttaa gtgttagttt
    12361 tccaacatca ataaacgcac tagataagat tgaaaatatt caaggagtag ataaatatga
    12421 aacagctggg cttatttcag ataagcaaga ttttacaaca gcagttctta tcaatgcaga
    12481 ttctacaatg gtagatggga ttgctgcaag tggtcttgca ggagtaaata atgcagctat
    12541 tttattaact aataaggatg atataccaga agcaacgctt caaagactga atagagttac
    12601 taagatatat gtaataggtg gagaaaattc tataagtaaa gatgtagaaa aaatgttact
    12661 tatgagaaga atgcaagtta taagaattga tggtgtagat agagtagata caagttataa
    12721 aattgcaggt gaaatagaaa aaattaaaaa cagtgataag atgtttttag taaatggatt
    12781 taaggatgaa gctgatgcag taagtgttgc atctgttgct tatagagatg gagctcctat
    12841 aatattgaca aaagatatac cttcagcaga agaagacaca gacttagacc catggtttgg
    12901 agtttctcct gttccagtat atgctattgg aggagagtct acattaagtg attacatagt
    12961 gagcagatat agagcaacta gaattggtgg tgttgataga tatcaaacta ataaaaatgt
    13021 tattgaaaag ttctataatg gagcaaaaga gttctatata acaagtggag acgatttagt
    13081 ttatgcttta gttgcatcac cacttgcaaa aaatgctcct gttgttttag tttcaaataa
    13141 aagtgataag agtatattaa gtggagctag taaagtaaca gcaataggta taagtgataa
    13201 gagtattata gagcaatgtt tggatgctgt aaagaagtaa tggagtgtta ttagtaaaat
    13261 gaatatatac attgaaaatt agtagattgt tcattttata atggtataaa atatatggga
    13321 agaggctatc aaatggagat aagcctcttt ttcattgtta aaattatgaa aagatacttt
    13381 attttatata aaaaagtatc ttatgaacta aatgtctatt ttgagacact atctactttt
    13441 cttatattat attatttttt atatggaata aatttcataa gtattatctt cctttaattt
    13501 ttctaaccaa accatctcta aaatatctag ttcttctaaa tagtctttct ctgttttgtt
    13561 atcatcatgt tttataagtt ctaaaacttc aaatgtaaat gctttttcac catatttttt
    13621 ccattcatta tttaattctt ttatcataca tgagccagca ccaagctgaa atttgagaga
    13681 gttaaatttg ctttttatgt cattactttt tccaaggtaa atctttttag taatatcatt
    13741 ttttattatg aatacacctg tgtcaaattt catttctttg tataattgtt ttaattcacg
    13801 ttttctatcc atgattttag atacctctct ttataattaa tatgtaatat gtaagtgtaa
    13861 aaatatatta catatcaatt ataaagagat taattttata tgtaaatagt ttttataaat
    13921 ttaattatga attagtcatc aataaacaag aaatttagct gtagaaatcc attataaagg
    13981 ctattataaa ttttaatatg ctaaaaatat aaaaagaata gttgacagtg tagaaaatat
    14041 ggaataaact aaaagtgtaa tagtaatatg aaattatata ttacattgaa aggtgtgaat
    14101 gattattatg aataaaaaaa tagaattaat agggaatttt gcaagtgatt ggattaggta
    14161 tagtgattat gaatataaaa ttactgaaga agatgaatta tatataattc caacagaaga
    14221 tgctgtgttt accatgtata acccatttga tgtagcagat gatataatag tagatataat
    14281 tcgcatagga caagaggcat tacaagatat atcaaaagag acaaaagaaa tgaaactaaa
    14341 gaaagaaata cttgaatttg taaaaaagta tggattattg ggtttaatat attcaagtgt
    14401 atataataga gatatagttg gagaagaaag agtactgatg attgaaaaga attatattac
    14461 aaaagagaat atattgagta cagatgaata tgtaaacatg tttataccat ttacaagtga
    14521 tgaagatgtg tattttaaaa agtatagaag gggtatagat gtatcaaaga gagaagaatc
    14581 tccaaagttt tttggaaaga gaccattgat tttagattta gtattttcaa agttctacac
    14641 tgaacaacta gattggattg taagttttgc taaagatatg tgtaagcatt ttaatcaaat
    14701 ttgtgtatat aaaaatacat ctaatctatt aactgaaagt gttactatta tgtctggagg
    14761 gtttaatcct caaaagatag gattttctat aagccaatta gataaaccaa ttattgcatg
    14821 ggagtttgat tctttgaaaa cagctattga gactatctat gcctttgcaa ttacaagtga
    14881 accttcttct ttaaataaat gcaagtactg tggtaaaatt tatattccta caaattctaa
    14941 atctcaatac tgttcacctt cttgtagaaa ttgctcaaat gtaaagaaaa gtagaagtag
    15001 agccaaaaaa gataaataaa attaataaat atatcattgt ttagatttta gtaaataact
    15061 actgaattaa aagttgttat agaaaattat atatctaaat gatggtattt ttttgttgag
    15121 aaaattgaaa agtttaattg agatagtaat tttgagttga caaaaaccac ttttaacact
    15181 actattataa gtggttggta aaataatata tactaattta agtatagcat aaaaaacaaa
    15241 aaaagtcaat gaaaaaaata aaaaaggaga atttaaaaat gatatcacct attattttga
    15301 gttctataaa tcagaattta aaggaaatag aaagaaatga actgcttgaa actaatatag
    15361 aatctggaga ttatggtcta gctctttctg aatctgatgt aaaagatata ataaattcga
    15421 gagataatac tttaaagggg tatggaagaa tagaattaga tataaaagtt acaaaacagt
    15481 taatagaaaa tatatataca tcacaatata caaatgtgga caattattta gaggctatta
    15541 atgatatgca agaaattttt tattatttaa aaaatgaaac tgatgacaag atatgtgatg
    15601 atgagattat agaaatactg ggtgaatttt atgaaaaatt ttcaggcaat atggacaatg
    15661 taagaggaga agcagacgaa tttgcaaaga aatttaaatt tggagaagtt taatatatga
    15721 taggagagat tttgtatgtt aaataattta agtaatatta atataaacga aaataattta
    15781 ataaagaacc aatattccat atctctactt aaggaatgtc taaattgtaa agtgatagat
    15841 gaaagagaag tatataatat acaacaagaa atatctctaa tacttatgga tttgattaaa
    15901 aaatatacaa atggtcaaag cacatcagta aaaacagaag ttgccgaaaa actgttgatt
    15961 tcaatatggt atgctattga tgcatatata aataagcttg gagatataga aaaaagagtt
    16021 gtggagttga ctgctaagaa tgttaaaaaa gtgtaccttg gaggaattga aatattagag
    16081 gaagagttag ttaggctaaa agatttttat aaactaatga tgagtagaaa attggatact
    16141 aattcaatag catataatga taccttgatg gagttatcaa gtttctttaa aagctatatt
    16201 gtaaaatttg aagctcagga cgttccagta agtatagatt atccattggc actagatgat
    16261 atggatgtta agggtattta ttatgtgaag aattatattg agagtataga tatagaaaat
    16321 agattttgta acctatttaa gaaaaaagat agagaaaaat tattttatga ctatggggtg
    16381 acttataaaa tagattgtga tgttatactt gtaaatttat ttgaacttat tatgaataat
    16441 tgtatttttt ctactatttt aggaaatgaa gtgactaatt tagaaatttc aaaatatgag
    16501 tatgaatttt tagaaaaaca atttaaaaat atgtacaata atattactga agataaagtt
    16561 atagactctg aagaaagtaa tataattgaa ttgattgaag tagatgatag ttttaacact
    16621 gaagtagata gtaatttgga ttataatgat tttgaagaaa ttaacagaga aattagaata
    16681 aataaaaaaa taactttact acttatggaa gcaattgaag tattaataaa aagattaagc
    16741 atagaagatg aagatgtaat aagatatatt aaaaaatatg aaaagatatt tattgaatct
    16801 gtgctaagct caataaaaag taacactttt aaaagtatgg taactgtgag taaaaataaa
    16861 aatgaacact tagaagatta tattgttgat gatgaaagta aattggatga tgaatcgttt
    16921 agagagatat tcaatgagat attagatagt acatctataa ttgaaaaaat aagaataata
    16981 aaggaaaata tcaatgctaa aaaagacttt atcgatattc ttgaatctga atgtttattt
    17041 ggagaagagt atttaatgtt gtttgcatca ttaagtgaat tagaattggc aatattgggt
    17101 agtgtagtat tttatgaaga tattagaatg agagaaatta atatattaga atttatgtta
    17161 aatgagaaaa ctgaaactac tttatggaag atagagtaca ttaaatttat gaagaggcaa
    17221 agtgaagata agatatactc catagaagag catatgaata aaattaatta acaaaaaatt
    17281 gtgaataaat agaatgttag tatataatat aatctgtaaa gcatatcaaa aatacatata
    17341 tttggaggaa aattatgaaa gagtatatca taaataatgt tgaagaaata agagaagaat
    17401 taattgattt atctaagaaa atatgggaaa atccagaatt agcctttgaa gaaaagtatg
    17461 caagtagcat tcaaaaagaa tacctaaagt caaaaggatt taaaattgaa gaagtagaaa
    17521 atttacctac tggatttata gcaagttttg gagagggtaa acctgtgatt ggcatacttg
    17581 gagaatatga tgctttacca gagctatcac aatgtgtgag tgcagaaaga aagccattgg
    17641 tagaaggaaa agctggacat ggatgtgggc ataacctgtt gggtgtagca ggtgtaggag
    17701 cagttgtttc tataaaaaaa ttaatagaag aaaataaatt taatggaaca ataaaatatt
    17761 ttggttgtcc tgctgaagaa gaaggtggag gaaagacagt tatgtgtata aatggctgtt
    17821 ttgatgatgt agattgtgca tttacatggc atccttttga tataaatgca ccttggagag
    17881 gtggaagtct ggcaaactta tctgtaaaat ttaaatttaa agggataaca gcccatgcag
    17941 ctcaagctcc tcataatggt agaagcgcac ttgatgctgt tgaaattatg aatgttggag
    18001 ctaattattt gagagaacat gttattgact ctataagaat gcactatgtt ataactaatg
    18061 gtggtggaag acctaatgta gttcctggat ttgctgaaag ttggtacttt attagaggta
    18121 aaaaagcaaa ggatgcagaa cacgttcttg atagattaat aaaagtagct caaggagcag
    18181 caatgatgac agaaactgaa atggagtata aggttacaga tggaatatat gactatatac
    18241 caaaccaatg tttgactgat ttagtttatg acaatatggt ttttgttggt tgccctaaaa
    18301 atactcctga agaagaagaa tttgcaaaaa aattgtgtga tactcttact agagaagaaa
    18361 gattaggtgt agcaacatca ttacaaaatg ataaatctat tgtagaaagc tatatacaca
    18421 aggaaatagt tgacggtgac aaagataaag gactagctgg ttctactgat gttggagatg
    18481 taagttatgt aataccagtg gctcaatttg caatggcagc ttggcctgtt ggaattgcaa
    18541 gtcatacatg gcagtcatgt tcatcagcag gttcaaatat aggattttct gctatgataa
    18601 attcagctaa agtactggct tgtagtgctt atgatgtatt tatggatact aaaattattg
    18661 atgaagcaaa aattgaattt gataaatcat tagatggaca aaaatttaaa ccattggtat
    18721 aattttggta ataaaatatt tctgggggga atagctatga gagaaaaagt tgaaaaagtg
    18781 cttgaggaaa agataaaacc agtattacaa agagatggtg gagatgtaga gcttatagat
    18841 gtaaatgaaa atggtgtggt tttagttaga ctacaagggg catgtagtgg gtgtccagga
    18901 gcgactatga caatcaaagc tattattgaa aatgttttgg tgagtgaagt tccaggagta
    18961 actcaagtat taggtgttta atagttagcg tttattaaat gtatgttata cataagtatt
    19021 taataaagaa gtaaaaaaaa tagtgaagca atatatatga aaaagtatga gaaagataat
    19081 aaaacattat agaaataatg aaatatttca tatgtatatg aagaaataag aaaaaat
//
LOCUS       DAEORZ010000074        18821 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEORZ010000074 DAEORZ010000000
VERSION     DAEORZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18821
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.074"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(48..239)
                     /locus_tag="KRH39_002275"
     CDS             complement(48..239)
                     /locus_tag="KRH39_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBG8512174.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            complement(387..629)
                     /locus_tag="KRH39_002276"
     CDS             complement(387..629)
                     /locus_tag="KRH39_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512175.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            841..2664
                     /gene="typA"
                     /locus_tag="KRH39_002277"
     CDS             841..2664
                     /gene="typA"
                     /locus_tag="KRH39_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBG8512176.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            complement(2754..3662)
                     /gene="trxB"
                     /locus_tag="KRH39_002278"
     CDS             complement(2754..3662)
                     /gene="trxB"
                     /locus_tag="KRH39_002278"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG8512177.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRNIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     misc_binding    4464..4695
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4803..6284
                     /locus_tag="KRH39_002279"
     CDS             4803..6284
                     /locus_tag="KRH39_002279"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBG8512178.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            6294..7187
                     /locus_tag="KRH39_002280"
     CDS             6294..7187
                     /locus_tag="KRH39_002280"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBG8512179.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            7311..8372
                     /locus_tag="KRH39_002281"
     CDS             7311..8372
                     /locus_tag="KRH39_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBG8512180.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(8527..9039)
                     /locus_tag="KRH39_002282"
     CDS             complement(8527..9039)
                     /locus_tag="KRH39_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBG8512181.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            9559..10494
                     /locus_tag="KRH39_002283"
     CDS             9559..10494
                     /locus_tag="KRH39_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBG8512182.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(10586..12457)
                     /locus_tag="KRH39_002284"
     CDS             complement(10586..12457)
                     /locus_tag="KRH39_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBG8512183.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            12647..13573
                     /locus_tag="KRH39_002285"
     CDS             12647..13573
                     /locus_tag="KRH39_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG8512184.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            13712..13858
                     /locus_tag="KRH39_002286"
     CDS             13712..13858
                     /locus_tag="KRH39_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512185.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(13963..14487)
                     /locus_tag="KRH39_002287"
     CDS             complement(13963..14487)
                     /locus_tag="KRH39_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8512186.1"
                     /translation="MSKPIDITIRPIRREDAQAFNKIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(14540..14764)
                     /locus_tag="KRH39_002288"
     CDS             complement(14540..14764)
                     /locus_tag="KRH39_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512187.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            14961..16085
                     /locus_tag="KRH39_002289"
     CDS             14961..16085
                     /locus_tag="KRH39_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBG8512188.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPSVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(16559..17383)
                     /locus_tag="KRH39_002290"
     CDS             complement(16559..17383)
                     /locus_tag="KRH39_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512189.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(17731..18786)
                     /gene="ispG"
                     /locus_tag="KRH39_002291"
                     /gene_synonym="gcpE"
     CDS             complement(17731..18786)
                     /gene="ispG"
                     /locus_tag="KRH39_002291"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBG8512190.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
BASE COUNT         6544 a         2854 c         2324 g         7099 t
ORIGIN      
        1 atgcttatac aatttagatg ctctatttat tataatttat ttaaaattta ttctttacat
       61 gcttcaaaaa actctgcttc tgtttccttt atttcaagct cattgtctat tattccaaca
      121 gcttcttttt taaatgctct acactgacct atacaaccag tctttacatt ttcttctcca
      181 accaaagttt ttagcttatt tacatccaca tttgaacagt ttgggcatac tcttatcata
      241 tttaagttcc tcctaattaa tgtctaatta atacttttat acccaaatat aaactttata
      301 aaagtttttt taatataaat tattaccata tataccattt atatcctatt gtgagtatta
      361 catattaatt ttaaatatta ttaatactac atgtacggtg gtatctgttg ttttctcact
      421 atttccaatt ccttaatttc taaagactta atcacattta ctttatagtt ttctttagac
      481 tttatataca aaacatcttc tatcttttta ccttcaataa atttatgaga ccacttaaga
      541 agttcttcaa aattacaaac ttcttctttt acaaaagtat cactttcact tgcaaaatat
      601 ttaattatta ttttggttac ttccaacata tatcatcctt tactctttta ttaatctttt
      661 tatctattaa ggttattatt tgtctaaaaa atttttttaa acagaataat tttcagatgc
      721 aaaactaaca gttttatcca atttaaacta tattaataca atttaatata ttcctccttg
      781 tacctaataa tatattgtgc tataattaat aagttatttt aatcgaaggg agcaacaaaa
      841 atgtcacaaa aacataaaat tataaatatc gcagttatag cccacgttga cgctggaaaa
      901 tcaacattgg ttgatgcttt cctttctcaa agcggtgttt ttagaaagaa cgaagtagtt
      961 aaagactgcg ttatggacag taacgacctt gaaaaagaaa gaggcataac tatatattca
     1021 aaaaactgtg caataaacta tgaagattac aaaataaaca tagttgatac tccaggacac
     1081 agtgactttt cttctgaagt tgaacgtgtt atgaaaactg ttgacacagt tattctatta
     1141 gttgatgcta gtgaaggccc aatgcctcaa actagattcg ttttacaaaa atctttagag
     1201 ttcggtttaa aaccaatatt atttataaac aagattgata aaaaagacca gagagcggaa
     1261 gaagtagtta atgaagtttt cgatttattt gtagacttaa atgcaacaga tgagcaatgc
     1321 gaatttccta taatatatgg tattgctaaa caaggtattg ctaaacttga aatggatgat
     1381 gatagtgaag acctttctcc tctatttaaa actattgtaa atcacgttga agcttatcct
     1441 aattacgata atgaacctct acagttccaa atatcagctc ttgcatatga tgattatgta
     1501 ggtagacttg gtataggtag aatttacaaa ggtactctta aaaacaatac acaagttgct
     1561 atatgtagag aagattctgt tgtatctaaa ggaaaagtat caaaactttc tgtgtatgaa
     1621 ggtttaaaac aagttgaagt tgatgaagct actagtggag aaatagttgt tatagctggt
     1681 attcctgata tatctatagg tgaaactata tgcgatttag atagtccact acctatggaa
     1741 atgataaaaa tagaagagcc tactctttct atgaacttct tagtaaacga ctctccattc
     1801 gttggaaaaa gtggaaaatt cgttacaact agacatttga aagatagact tgaaaaggaa
     1861 cttgaagtaa atgttggtct taaagttgaa cctcttgata ctactgatgg atacaaagtt
     1921 tctggacgtg gagagcttca cttatctata ctacttgaaa atatgagacg tgaaggttat
     1981 gaagttggtg tttctaaacc tgaagtttta atgcataaag aagatggaaa actaatggaa
     2041 ccaatagaaa gagttgttgt aaactgtcct gaagtttact ctggtactat tataaatgaa
     2101 ttaaacatga gaaaaggtat gatggagtct atgtcaatag aaggagacta tgtaaaaata
     2161 gagttcttag cacctactcg tggtctttta ggatatcgaa gtgaatttat aaacgctact
     2221 cgtggagaag gtactttagt tcgttcattt gaaaaatttg aagaatttaa aggagaaata
     2281 ccttcaagag gaaatggtgt tttaatagca caaggacctg gtgttactat ggggtattct
     2341 cttaatgcct taagtgatag agctgtaatg tttgtagacc ctggtgtaga agtttacgaa
     2401 ggtatgataa ttggtatgaa ttccagaaaa gatgatatgg tagtcaaccc ttgtaaaaac
     2461 aagaaaatga gtaacgtacg tgcttctggt tcagatgatg ctataaaatt atctcctcct
     2521 agaatattta ctcttgaaga agcactagag tttatagaag atgatgaatt agttgaaatt
     2581 acacctgatt ctataagact tagaaaaaga ttcttaaatg aacatgatag attaagatat
     2641 aacaaatcta gacaaggtaa ataaaacgta catttacctc taattaacaa aatagccata
     2701 tagtactgaa aaattatttc agtttatatg gctattttta tttgaaattt atattattca
     2761 acatttatat aattagctgc ttgaacggct gcaatagctc catctgatgc tgctgttatt
     2821 atttgacgta aaaacttagt tctactatct cctgctacat atacaccttc tactgatgtt
     2881 gtacaagact catctgaaat tatatatcca ccttcatcca aatcaagaac attttcaaac
     2941 attttattat taggtttaag acctatagca ataaaaatac cactaacatc tatatttctt
     3001 ttttcttcag tctttaagtt ttttacttct attttcgata cagtcttttc tccttctatt
     3061 ttttctacac catgactgta aagtatctca atattctctc ttgctttcac agatttctcc
     3121 aacacttctt cacctctaaa actatctctt ctgtgaatta aatacacttt tgtacaatta
     3181 ttagctaaaa ataaagcatc ttctagtgca gtatttcctc cacctactat agcaacttct
     3241 ttatctttaa aaaaggctcc atcacatgtt gcacagtaag atactcctct acctgtaaat
     3301 tcttgttccc ctgcacaacc taatttacgt ctttcaactc catttgcaag aataacagcc
     3361 tttgctttat gttcacctga agaagttttt acaaccttta cttttccctc taaatttatc
     3421 tcttcaacct catcaaactg aatatcaaca ccttgagcaa ctgcttgatt gtatatatta
     3481 ttagaaaatt ctaccccaga aatcttttga actgctggat aattttctac ctcagaggta
     3541 cttgcaactt gacctccata aatatttttt tcaaatactg taacgcttaa ccctgctctc
     3601 atagcataaa ttgctgaagt taaacctgct ggtccagcac caattacaat gatatctacc
     3661 ataacgttta cctctcttta tattattatt tcttgtatat tattctcatc attcttatac
     3721 tttactatta ttatccatat atactataaa caaaacatta ttttttatta attaacttta
     3781 ttttacatat atataaaaaa actgcaaata aaaattaact tttatttaca gttctcactt
     3841 atttatataa ttcaaaacct tattggttat catttacagt gtcttctaat atattgcttt
     3901 ctgatatata tgatagaatt gaaagtccat acataaccaa aactaatgct gctgttataa
     3961 gtactattcc catatttaat acctcctttt gtttacagta tactattatt atgtgaaata
     4021 tttatgaatt taatttgaat atatatgtaa tttcacacta acaatttctt caaattaaac
     4081 attattaact atatactttt gttgaaccaa ttatgattaa tagtttaaat attagcacta
     4141 tttacttata tttatatgat ataccatata attacatttt tcaaattaaa gttttgtaaa
     4201 ctttttttgt aatatagtgt agtataaaat tgtaatatta cagatattta atagttatct
     4261 tttgattact ttcattaaaa acagcttcat cactatgaaa atctaggttt ttaaactttt
     4321 aaaaaagtta aaaaaatatt tttctaatac tcaatttgat tacaattttt ttacaattta
     4381 aaaaatattt tttccaaatt tttatattaa tacttgactt tgtctgtttt ataaactaga
     4441 ataattaata atattaagaa tattgaaaag cattgattag gaagagtaaa tctgaatcga
     4501 ttaccagaga aggaaactac tagctgaaat gtttccctaa ttagaaggat tgaaaactac
     4561 ctaggagctt ctatccgaag ttatcttaat gattttagta ggaatagacg tttttattac
     4621 ttgcgttaaa gtatcaagtg gattttttaa aatcaatttg ggtggaaccg cgggaaacag
     4681 attctcgtcc cttttttagg gatgagggtc tttttttatt acctaaattc agtttttaaa
     4741 atattctgta tctaatttaa ttagctaatt aaaaatttaa aatatatgga ggattataaa
     4801 aaatgtatta ttcaagtacg agaggcaccg aagaaaaagt aactgcatca caagcaataa
     4861 taaaaggaat atcaagtgat ggtggtcttt atgttccaag tgaatttccc aatgtaaaaa
     4921 atgaacttat aaatttagta aatttaacgt attctcagat agcatttttt gtacttagta
     4981 aatttttatg tgattttact gaagatgaaa ttaaaaattg tatagaaaat gcatatgatg
     5041 aaaagtttga ttgtagtagt atagctccat taaataaagt aaatgatact tatttcttag
     5101 aactatatca cggtccaact ttagcattta aagatatggc acttactata atgcctcatc
     5161 ttcttaaaac atctataaag aaagataatt tagagaaaga tgttgtgatt cttactgcta
     5221 catctggaga tacaggcaaa gctgcacttg agggatttaa agatatagat aaaataaaga
     5281 taattgtatt cttcccagaa gatggtgtaa gcccagtaca aaaacttcaa atgaaaactc
     5341 aaactggaaa aaatacatat gtagttggta taaaaggaaa ttttgatgat gcacaatcag
     5401 gtgtaaaaaa gattttttct gataaagaat ttaatcaaaa actattggat aataattata
     5461 tattatcttc tgcaaattca attaatatag gaagacttct tccacaagtt gcatactatt
     5521 tttattcata tatgaaatta gttagcaata gtgagataaa tttaaatgat aaaataaatt
     5581 ttgttgtccc aactggtaat tttggtaata ttctagctgg atattatgcc aaacaaatgg
     5641 gtcttccaat aaacaaattg atatgtgcat caaatgataa taatgttctt tatgatttct
     5701 ttaaaactgg tgtatatgat aaaaatagaa cattgaaatt aactacttct ccttctatgg
     5761 atatattaat ttcaagtaat ttagaacgat tactgtttga aatatcaaat agaaatactg
     5821 atattgtaaa taagctttta tcagacctta gtgataaagg agtctacaaa ataagtgatg
     5881 atatggaaaa aaatttagcc tcattctatg gagaatattc aaatgaagaa gaagttaaaa
     5941 gcacaataaa caatgtgttt aaaaattata attatttgat agatactcat actgctgttg
     6001 catacaattg ttatgaaaaa tataaaaaag aaacttctga taacacaaaa actgttattg
     6061 taagtactgc aagtccattc aagttttctg aagatgttct aaagtctata gattgtgatt
     6121 ttaaagacct tgatgacttt gctataattg atagattatc aagtatttct aaaatagata
     6181 ttccaaaacc tattaaaaat cttaaaaatg cagaaatact acataaagat atttatgaaa
     6241 aagatgaatt aaaactagct ataaaaaaat tcttaaaggt ataggtgatt attatgttag
     6301 agattatagt tcctgcaaca agtgctaata taggtccagg atttgattgt cttggaattg
     6361 cgttaaatat atacaataaa ttttatgttg aagaaattga aagtggtctt gaaatagaag
     6421 gttgtgaaga tgcttacaaa aacgaaaaca acttagttta tacatctatg aagtactttt
     6481 ttgatagagt taaaccagaa aaaatacctg ctggtatcaa aataaaaatc caaagtgaag
     6541 tacctatatg tagaggtctt ggcagtagtg cctcttgtat agtagctgga gttatagctg
     6601 caaatgctct atcaggtgct aatttagata aaaatcaatt actaaatata gcttcagaaa
     6661 tagaaggtca cccagataat gttgctcctg ctattttagg aaatatgatt gtatctgtta
     6721 ctgataatga gaatatacat tatgatatta taaaaattcc tgaagaactt aaattttgtg
     6781 caatgatacc taattttaag ttatcaacag aaaaagctag aggtgtttta ccaaaggaaa
     6841 taccatattc tgatggggta ttcaatgtta gtagagttgc tttactaata tctgccttac
     6901 taaacaaaaa ctttgaccta cttaaagtag cttgtcaaga caaattgcat caagattata
     6961 gaggaactct aattgagaac tacaatgata ttgttgaaaa atctgaacaa ttaaatagta
     7021 ttggagtatt tttaagtgga gctggaccta ctataatgtc tttaataaaa gaaaatgatg
     7081 atagttttgt ggataatatg aaaaattacc tacaaaaact aaaatctgat tgggaaataa
     7141 aagagttatg ttgcgattct aatggagctg ttttaaatat aatataatta tcgtcttcta
     7201 tacaaaatat aactactctt ttattataat ttgtagtata ataattagga ctaggtaata
     7261 atattaagta ttataatcta gttctttaaa catgtttaca ttgaggaggg attatcataa
     7321 aagagttaat aattagagat ttagttgcaa aagttcctat aattcaaggt ggaatgggcg
     7381 ttggtatatc taggtcatca ctagctggtg cagttgctaa gcttggtggt gttggtgtaa
     7441 tttctggagt tcaaattgga tatgatgagg aagattttga aactaatact ataaatgcaa
     7501 atttaagagc tataaaaaaa catatttcta aggccaaaga aatctcaaat ggtggtatta
     7561 ttggtattaa ttttatggta gctatgaaag aatatgaaac atacgtaaag gaagctgtta
     7621 aagctggtgt agaccttata atatctggag ctggattacc taataagcta ccatcattag
     7681 taaagggaag taatgtaaaa atagcaccca tagtttcaac tgcaaaagct gctaatgtaa
     7741 ttttaaaaat gtgggataga aaagagaaaa ctactgctga cttaatagtt gttgaaggtc
     7801 caaaagctgg tggtcacctt ggatactcta acgaggaact tgataacata gattctatag
     7861 actatgataa ggaatttgtt gaaattttaa aggttgcaaa tacttatgga gaaaaatttg
     7921 gtagaaacat accagtagtt gctgctggtg gtataacttc tagtagtgat gttaaaaaat
     7981 atatagatat gggtgcaagt ggtgtacaag ttggtacaag atttgttgct acttacgaat
     8041 gtgatgccca tgagaacttt aaaatggcat acataaatgc tagtgaagaa gatgttcata
     8101 tagttaaaag tcctgtaggt cttcctggac gcgccataag aaacaaattt atagaagaag
     8161 ttaaaataaa tcgtccagaa ataaagaagt gctataattg tttgattcct tgtaatccaa
     8221 aagaaactcc ttactgtatt tctcaagctc taatcaatgc agttaaagga gatgttgaaa
     8281 attcactttt attttgtgga aatgatgcct ataaaattga taaattaagt actgttgaag
     8341 atgttatcaa tgaattgatt tctgaattat aatttaaaga aaactaaatt aagattttat
     8401 caagctaagg agtttaaata cgttatttat taatctttat aaacatattt atgtccttag
     8461 cttttttgtg tcaatttcct tttatggaaa ttttaattta attagagata gtattaacct
     8521 ttataactaa tcttctttta caagtacatt ggttatttca gctatatata aagtatgata
     8581 atctttttgt ggataccatc ttccatctat accttcagca ataaaacatt gaggttcaag
     8641 tttctgacta tacatttttt tgcatataat agccatttta gattctttaa aataaggtgt
     8701 atcatttaag tgttctatat taaatccaat ctgtgatatc ttatcctctt ctctaccaga
     8761 tacctttcca caatatgaaa gctctttacg aaaatcttca tcaaaaaatg taagagaaaa
     8821 tttatctgta ttatctataa attccttagt ataacgctgt ggtcttatta ctacaaaagc
     8881 aacatctttt ccccacataa ctccaagccc tccccagcta gcagtcattg tattcacttt
     8941 accttcattt tctgctgtta taagtgtcca atctttacct atcaatccaa atggattatt
     9001 tttaatctct tctgccaaaa tttctttaaa atcactcata tctctaataa cctctcattc
     9061 tcttatattt atattccttt tattttcgtg ataaattata aatgaagtct agttttgaaa
     9121 ataaaatact tttttaaata tattattgca aataagtaaa tatatatata cactcttatt
     9181 ttttaatata tttaataatt tcactcagaa tatatatatt ctttactcaa attaaatata
     9241 acttatatac tttatgtgta aattatggta aaaatattat ttaatcaaat caaaacttga
     9301 tttatatacc tggaaaaggt attatattaa agagtgctta aaatacgcat ttcataaata
     9361 gctatatata aaataataaa taaggagagt gatgggcaat ggatactagt ccgttgcgga
     9421 gagaattttt tacattttca tcacttaata atatctaaaa tcaaaactaa tacatatatt
     9481 cagttatata aattagttaa ttaatagata ctattaaatg atgagattta tataaaactg
     9541 aataaaggat gtgttgccat gataagatat tacaaaacta tagattcaaa attagagaaa
     9601 cttagttttt ttgaagatgg ctgttggata aatcttgttg aacctaatca tagtgaaatc
     9661 aacgaaattt ctaatttatt aaatatagat gtagaaagca tagaatctgc actagatgaa
     9721 gaagaacgct caagaataga tgttgaagac aatcatactc ttatacttat agatatacca
     9781 gtagacgaaa gtgactctaa ttcttctcat tatactacaa tacccttagg tattatacta
     9841 actgaagaag ctattgtaac agtttgtgat gcacaaacta agattttaaa tgactttata
     9901 gttggtcata ttaaagattt ttttacgttt aaaaagactc gctttttgct tcaaattctt
     9961 cacaaaaatg cagcttatta cttgcattac ttaagaaaaa taaataaaat gacaatcata
    10021 atagagcgag aaatttacaa atcaatgaaa aacaaagaat tggttcaatt attggaatta
    10081 gaaaaatctt tggtatactt ctctacctca ctaaaatcta atgaacttgt tttaaacaaa
    10141 atggttagaa ctgctggtat aaagaagtat ccagatgatg aagacctatt agaagatgtt
    10201 atagttgaaa atagacaggc tcttgatatg gctaaaatat atggagatat attgagtaga
    10261 attatggatg ctttcagtgc aattataagt aataatcaga ataatgttat gcaaatttta
    10321 acagtagtta cactaatttt ctcaatacct acaataatat caggattctt tggtatgaat
    10381 gtcatcaaca tgccattttc taataaccca aacggatttt ggataatact tcttattacc
    10441 tctataatat gtatagtgat tacatttttc atgtcaagaa ataagctatt ataaaatatt
    10501 aataaaaaag ccattagaat taattgattt acatttatcc taatggcttt tatatattta
    10561 aatctatata atcttataat actttttaac tatcatgttc ttcatctata atcatttcag
    10621 tttcaagccc ttctagcatt gaatctaaac tttcttttat atttagtata taaattggta
    10681 catactcttc ttcatctatt acctcttctt gcaacttaaa tggaggaatt aatacttcat
    10741 caaagtctat atttaaattt ggaaagcctt ctactatctc ttcacaatta tcgtactctt
    10801 catttgctaa agaaatgtac atatctaggc tctttttata ataaagacct gcaagcatat
    10861 actcacactt cttacttcca tatttaatac cttctttata atatttctca gcttctgcgt
    10921 catcatttaa ttttaaataa atttgaccta aattatatat tgattcaaca caaccttgtg
    10981 ctctagcctt ttcataccaa aatttagaat ttacataatc tttatagaac atatcataaa
    11041 ttaatcctac catatgttga gcatacacat tattttcatt ggcaggtgta tcaaacatca
    11101 ttttagactc ttccaattta ccttctctaa aataaattct acctagatgt atacgagcat
    11161 tcatatggtt tttgctactt gccatttcaa aatactttct agcatttttt gtatttccta
    11221 agctctcata tatacacccc aatctataac tagacctcgt atctccattt tcagatgctt
    11281 tcttatacca acttattgct tcttcaatat tatttaaatc ttcatagata tttcctaatt
    11341 tgatttgaca agatacatta tttggaatcc tcgaataata cgaaatcgct ttttctatat
    11401 cttgattctg ataatataaa tcccctagat tttcaaggga acttttgcat ccaacagcta
    11461 tagcatcctc ataatattta atagcatttt cataatcttt ttcttcaaaa tatatacctg
    11521 ccagattatt ttttgcttct gttatatcat cttcagcagc cattttataa taagtttttg
    11581 ccttctctat ctcattttct ctattatata aattagcaag tctatactta gaaagtgtat
    11641 gatttttttc tattgctttc tcataataat aaattgcatc tcccagctct tcttttctat
    11701 catatacata tgcaagccta tactcagact tcacatcatc tctatcagca ctctttttat
    11761 aatacttctc tgcatcttct aaattatcat ttatttccat aatcataccc aagttaaagc
    11821 aacctttcat atgacctgac ttgtacaact gtttatataa acttactgtt gatttattat
    11881 ctttcttttt tccatttaat attgctaaat tatattttgc atcatcatct aaaagcacag
    11941 aacctttttt atagtattca tatgcttcat catctttctc caaaaaagta tataataccc
    12001 ctaaattaaa acaagcttta cctaaattct tgtctgctgc tgtttttaac catttttcac
    12061 accttacaaa atctttcatt ttaaaatggc atacacctaa attattttgt gcttgtaaat
    12121 aacctaaatt tgctatcttt tcataccaat atatagcata ttcatagcca tctaacttat
    12181 agtacatatt acctaaatca aaagcagcat cttcacttcc acttttatat gccattgaat
    12241 accacttttc tgcttcttta aattgttttt tactttcata tatatttcct aaagtatatt
    12301 gagattttac gtaattatcc aatgcagcac tcttatacaa ctctattgcg ctttccatct
    12361 tacccatctt ttcaaggagg gcacccttag tatgtacgac tttcgggtca ttagatatat
    12421 tttttatttt ttttatagaa aattttgtat ttttcattat tcgctaccta cccattttat
    12481 aaaatatatt ctctcaaaca ttattataca ttataatcta caattaatgc tataatattg
    12541 aatttttacc ttttgtcact atattgtaac attaaatttt aaattttata atcattagat
    12601 tttattatgt aattggtata taatgtatat atagggggga cattttatga atttcagcaa
    12661 tatttttatc caagttgcag ttttatttat aattatttta gttggatatt ttgtcagaaa
    12721 attcaactta ttagatgacc actgcacatc taagctttca actttgacaa tgacagtttt
    12781 tttaccatca atgataatta gctccatgca aattaacttt gatagtaaaa tgatacaaaa
    12841 aatactattg ttattattta catctttgat tatgtatata gtttcaatta taattgcatt
    12901 tttattgaaa tacattttaa aatgtaatga taaaaaagat ttgggaatat atcagtatat
    12961 agttgttttt tcaaatgttg cttttatggg ttatcctgta attgaagcag ttttaggtca
    13021 tgaagcaata ttctatactg caatattcaa tttgcctttt aacttatttt ctttcacttt
    13081 aggaatttat cttttatcta aaggcagtac aagtagaggt ttttcaataa aatcacttat
    13141 aagccctgct acaatagcta ttgttattgg acttttttta tttgtaacag gacttaggtt
    13201 accccaattt ataaacgagc cacttgagat gttgggaagt ataactacac caatatccat
    13261 gattatcatt ggaagcttac ttgctaattc ttcagctctc gattgttttg tcaacaaaaa
    13321 attatatata gtcactttta taagattatt ggtacttcct gtgattgtgt attttattct
    13381 aaaaggatgg ataaatgata agatgatttt agctatacca gtagtaattt cttcaatgcc
    13441 tgctgctgca aatactgcta taatggctaa tcaatatgac tccaatataa cactagcttc
    13501 acaatgtgtg ttttttacaa cactattttc agttatatct atacctttta taagtatttt
    13561 actcttatct tgatttattt ttttatgtat tatataataa catattcaat aaattacaaa
    13621 tataaacatt ttattgtagt atattgatgg aattttttcc tagatatttg tcaataatat
    13681 ctatatgtat tatagaatag gaggcttaaa tatggatatg gatttactct ttaaaatagt
    13741 atttgctata gttgctatag gtgtttctgt aaaaatcatt aaaactgttg ctagtttagc
    13801 ttttaaaata gcgttgatat tgctaataat tttatttgta tatagaatgt ttttgtaaac
    13861 atttaaaaat aagtttttta acacaataga gaagtaaaat agattatctt taatgtaaat
    13921 tttttattaa agacactcta ttttacttct ctattagctt ctttaaatta aatttttatt
    13981 atatcttccc ataatataaa catcttcaaa ttttccatct ctgattacag catatttttt
    14041 agttccctct atttcaaaac ctagcttttt atataaattt aatccctttt cattatccac
    14101 aattacttct aatcctactc tagttaacat aatccaatta tctgctatat ctaaaagttg
    14161 ttccataagc ttcttaccta ctcctcttcc ctgataattc tctgcaacac ttattcctat
    14221 ttctgcgcta tgtctcaatc gtggagaaga atttacatgt aacccagcaa aacctattac
    14281 atctttttct ccattattat tagtttcagc tacaaacata taatcatttt ctcccaaaga
    14341 agctaacata gcttcagttt tatcgactct gtcactcatt aatgccaaag tatttctcat
    14401 aacactgtat gttcttctaa ttttattaaa agcttgtgca tcttctcttc ttataggtct
    14461 tattgttata tctataggct tactcatatt ggattccccc tcaaacataa tctttatttt
    14521 taattaaaat tttacatctt tattttatac tgttcaaatc tatgtatgct tctacctgag
    14581 gtacatatat catattatct tttatatgtg tacttccaaa ttcttttaat tctgtacctt
    14641 ctttatcctt agttatttta tatctaatgg attccatacc ttcaactact tcagcatgaa
    14701 aaaacatacc ttcatattgt ttaaatctaa aattaaaatt gtctttagta agttttatta
    14761 tcatattttt atctactcct tatataattg atatttttct tattattagt tgtatatata
    14821 ctgcttattc atattaatat attataactt atcatttatt tagtataaag ttataaatag
    14881 atatatttat acttatttta caatctttct tcaattgata tataatatgt ttatataaac
    14941 taatgtatgt gaggtgaaat atgagcaaaa aaacaaaata tttttcttta attctctttc
    15001 ctattgcttt gcttttaaat tttatagcca gtaaagttcc aaatattgtt gaaaaatatt
    15061 actcccaatt catagataaa ataattgtac aaatattgag taaagtttct ggaattttcc
    15121 cattttctct ctatgagatt acaatgtaca taatagttat atctatcttc ttatttttat
    15181 gttgtacttt atctacaatt tttaataaga aaaaaaaatt aaacatatat ttaaaaaatt
    15241 ccattttaaa tatattatcc attgtatcaa tagtctactt tctttttgta gtgctttggg
    15301 gaataaacta taataggatt ccactagaaa ccacacttat aaataactat aatttaaaaa
    15361 acaatacctc tatccaatca aaacaatata gtgttaaaga gcttactaaa ttatataaat
    15421 tccttgtaat taaagcaaat gaaactagaa aactcacatt acaagataaa aatggtattg
    15481 taaaatctaa tacagattat aatggtgtca taaatagagc tcaactagga tacgataata
    15541 tcttggatat attacctagt gttagtggta gttattctaa gcctaagtat attatttcat
    15601 caaatctaat gtgctataca ggaataactg gtatatactt tccttttaca ggtgaggcaa
    15661 atgtaaatat agcaatacct gacttatata ttccttgtac agttgaacat gaaatggcac
    15721 atcaaagggg atttgctagt gaagatgagg ctaattttat agcgtatctt acttctataa
    15781 aacaccccaa tattgatttt aactattctg gatatatttt agctttaaat tatactgctt
    15841 ctgctttaag taaggttgat tataatgcat atgttgatat ttctgctggc atttcagatt
    15901 ctgtccgtag agatttaaaa aatgaaagtg aattttggca aaaatatgaa ggtaaaatta
    15961 atgaaatttc taatgagttt aacaattctt acttaaaagc taatggcgtt actgaaggta
    16021 ctcagagtta tggtaaaatg gttgatttat tgctaactta ttatgaatta tatccatata
    16081 actaaacact acaattcaaa attttattca tatacagctg atattaaatc agctgttttc
    16141 tatttaaata aattatttaa cttcaagcaa tcttatataa tttgatagta cttatactat
    16201 ctgtataaca tttcaattct aactatttta gttaaaacat ttctattatc ttttttaata
    16261 aatgttttaa ctaaaatttt taaaacataa aacttccaca ttatttcact atctatataa
    16321 aaatctaaaa attcacatat aaaattacat aactaaaaaa tcataaattt atagaaaaca
    16381 tatattcaaa ataaaaaagc atgtatcaaa acttgacaca tgcttataat cttatgtata
    16441 tatatcattt tgtcacaata aaaatttact tgtaacactg atatttttaa acaaaatagt
    16501 ccttatttaa ttaaattaac attaattaaa ttcgtactca ttttgctatc attaaacatt
    16561 acttatagta attttctaat acactatttg aaacttcctt cccttgaaaa cttttcaacc
    16621 aattctcata tgctgagttt ttccatacac taccatcatt aaatgttact tgttttacac
    16681 aagatataat gtactttaca ccatgctctc cagttgattg tttccattta tcaaacaata
    16741 gaaaggcatc attttgattc ttacctggtg ctattgcatt ttgctttgca tattctttct
    16801 ccatttgttt ccattctttc aaccaatttt ctacccatgc cttgccttgt tgtttttcaa
    16861 acatacttat tatatcctgt ggtggtgatt gttgcatttt tctataagta tgtgaatatg
    16921 attttggaga cacaggagat atgcctgtaa caataccata gtctacacca gctgggctaa
    16981 atccaacact tcctacttcg ccatctgctg ccacattttg agcatcccaa tatagctcaa
    17041 gtggttttcc atttttatca tatgctaagc atgtaatttc ataatccata attgcctttt
    17101 cactgctatt tgttaagctt ataacattgc taggccaatg atatggagta tcatcatact
    17161 gtattttgct attatttata aataatggaa tattctcatt ataaacaaga cctttactac
    17221 tttgaatatc tgataccttc tttgtgacag tacttaccgt atttgctaag tatacatttg
    17281 ttttttctga attggtcgta ctaagagaaa atgttgttgt cgttccagta atcagcaaag
    17341 caacaactaa aataggtaag ctagatattt ttttatatct cacagttctt cacctctttt
    17401 tctattaact tataataaaa caatttacca aaacattaaa atctatgtta acataattta
    17461 ccaactgttc tagtaactat atcctatcat agttactatc aagtttcaat actattgatg
    17521 gaaattttca ctatattaaa tcttttttta attttataaa tttacaatat gatttaactc
    17581 atacatatat agtcaaatta gaatataagt tttttacatt taatgaagtt tataaaataa
    17641 aaaatgacta gataattaaa tactgtaaat aaaaacttat tacaaaagta tttaaaaatc
    17701 tagtcaatta atatatatat gaattgtata ctataatttt tcaatctctt ctagcaactc
    17761 atcaactaat ttgttaccat caatcattct aactatttca ccctttttaa ataaaagacc
    17821 ttcaccttta cccccagcaa tacctatatc tgcctctctg gcttcaccag gtccatttac
    17881 agcacagccc ataattgcaa cagtgatatt tttttccata ctaccaatct tttcttcaac
    17941 ctcatttaca acactaatta agtctatatt acatctacca catgtagggc atgatataac
    18001 tttaatctta tcatttaaaa gtcccaaact tcttaaaatc tgtttaccaa caataacctc
    18061 ttcaacaggg tcgcctgtta aagatattct aacagtatct ccaattcctt taagaagaag
    18121 tgctcctacc cctatagaag actttattgt tcctttgtga acacttccag attctgtaat
    18181 tccaatatga agaggataat caactttttt tgatattaat tcatatgcat caacagtttt
    18241 ataaatatcg gatgatttta gagatataac tatattatga aaatctaagt cttcaagaat
    18301 cttaatatgt cccattgcac tttcaactaa tgcttcagca gttgcagagc catacttttt
    18361 taaaagttct ttctctaaag aacctccatt aactccaatt ctaattttta aatttctttc
    18421 cttgcatttc tcaactacca tcttaactct ctctatactt ccaatattac caggatttat
    18481 tcttattcca tccacacctt gttctatagc ttcaagggct agcttataat cgaaatgtat
    18541 atctgcaata attggtatat ttacactaga ctttatcttt gctatatttt tagcagcttt
    18601 aatatcagga acagcaactc taactatatc acaaccagct tcctctaatc tctttatttg
    18661 agctattgta gcatctgcat ctcttgtatc agtatttgtc atagattgta tacttatagg
    18721 attttctcct cctattttta cagttcctac acttacttct ctcgtctttc ttctctcgta
    18781 actcatattc cacctctata tattatttta tattgaatct c
//
LOCUS       DAEORZ010000075        18757 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEORZ010000075 DAEORZ010000000
VERSION     DAEORZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.075"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            974..1813
                     /locus_tag="KRH39_002292"
     CDS             974..1813
                     /locus_tag="KRH39_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBG8512191.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            1810..2991
                     /locus_tag="KRH39_002293"
     CDS             1810..2991
                     /locus_tag="KRH39_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512192.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            3295..4197
                     /gene="ptb"
                     /locus_tag="KRH39_002294"
     CDS             3295..4197
                     /gene="ptb"
                     /locus_tag="KRH39_002294"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBG8512193.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            4219..5298
                     /gene="buk"
                     /locus_tag="KRH39_002295"
     CDS             4219..5298
                     /gene="buk"
                     /locus_tag="KRH39_002295"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG8512194.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            5288..5965
                     /locus_tag="KRH39_002296"
     CDS             5288..5965
                     /locus_tag="KRH39_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8512195.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            6008..6223
                     /locus_tag="KRH39_002297"
     CDS             6008..6223
                     /locus_tag="KRH39_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8512196.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            6252..7331
                     /locus_tag="KRH39_002298"
     CDS             6252..7331
                     /locus_tag="KRH39_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG8512197.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            7331..8083
                     /locus_tag="KRH39_002299"
     CDS             7331..8083
                     /locus_tag="KRH39_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG8512198.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            8084..8641
                     /locus_tag="KRH39_002300"
     CDS             8084..8641
                     /locus_tag="KRH39_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG8512199.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            8811..10157
                     /locus_tag="KRH39_002301"
     CDS             8811..10157
                     /locus_tag="KRH39_002301"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBG8512200.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHDADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            10424..12256
                     /gene="glmS"
                     /locus_tag="KRH39_002302"
     CDS             10424..12256
                     /gene="glmS"
                     /locus_tag="KRH39_002302"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBG8512201.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            12707..14002
                     /locus_tag="KRH39_002303"
     CDS             12707..14002
                     /locus_tag="KRH39_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBG8512202.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            14465..15202
                     /locus_tag="KRH39_002304"
     CDS             14465..15202
                     /locus_tag="KRH39_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBG8512203.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            15223..16476
                     /gene="murA"
                     /locus_tag="KRH39_002305"
     CDS             15223..16476
                     /gene="murA"
                     /locus_tag="KRH39_002305"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBG8512204.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            16563..17627
                     /gene="spoIID"
                     /locus_tag="KRH39_002306"
     CDS             16563..17627
                     /gene="spoIID"
                     /locus_tag="KRH39_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBG8512205.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            17693..18361
                     /locus_tag="KRH39_002307"
     CDS             17693..18361
                     /locus_tag="KRH39_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBG8512206.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            18459..18719
                     /gene="spoIIID"
                     /locus_tag="KRH39_002308"
     CDS             18459..18719
                     /gene="spoIIID"
                     /locus_tag="KRH39_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBG8512207.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
BASE COUNT         7553 a         2021 c         3613 g         5570 t
ORIGIN      
        1 taagaaaagt ttactataaa ttatagtaaa cttttatatt gtttaaaaag acacaggaaa
       61 catgtatatt acctgtgtct ttttatttat aaactttaaa ctatgtaaaa attacagtat
      121 attctaatag agttacagta tattcattaa tattgtaaac atacggaggg taatgtataa
      181 tagtctacaa gatgtttatg aaggcatcag ttaaatataa ctaattagta agagctatat
      241 acattatatt gtatacttta tggtatttat atattgaaat aacttatgaa attatttata
      301 aagagattca tgagcatatt tttgtctaaa caataagtat aagaaattat aaaaaattag
      361 agactatagt gaatttacaa tttaatgtaa tatttataag ttatactaaa taaattatta
      421 tactgtgaaa tggatatata actttaggta ttgtgtatta ttttcactgt aggagattat
      481 atctagttat acataataag ttttaatgat agtttatagt aagatattaa aataaattta
      541 tctaatctag atatgaaata agttattact ttaatataaa aatgaatcta atggaataat
      601 attgatgata agtaatattt agattttgta tttgtttata gttaataagt ggattataga
      661 atttataata attaatatat attaaacaaa gatatattaa gatattttag aacataaaac
      721 tgaattaagg cgcgagtact attttgtatt cgcgccttga tggtttgagt tatttgagta
      781 aaaactaact ttaataagaa atatgtattt attaagttga tatttttaat gtgtttagag
      841 attatttaac aatctccaaa tataaaacta gaattattta cataaaagtt aaatagtttg
      901 tatagagtta gatttatata gtataatact tagtgtaaat aattaagaag ttatcaaaca
      961 ggaggataaa actgtgttag atataaattc ttttttaaat ggatttttta atataatatt
     1021 aagaatgagt atatatgatt taattgacat atctatagta gcgtatattt tttacaaaat
     1081 atttatgttt ataaaagata caagagcaga acaagtgttt aaaggtataa tatttctttt
     1141 attagcaacc caacttagta acacctttaa attgcatacc gtttattgga tatctcttaa
     1201 agctttagat tatggagtta tagctgctct tataattttt caaccagaat ttagagcagg
     1261 acttgaacat ataggaagag caaagtttaa tttatttgga aagaatgtaa atacttctga
     1321 agaaactctc aatagaaata tagaagaaat tgtagaagct ttatattcac tttctagaca
     1381 aaaaatagga gctcttatca taatggagag agaaactaga ataagtgata taataaatac
     1441 aggaactatt attgatgctg aaatttctag gcaactatta ataaatatat ttataccaaa
     1501 tacaccatta cacgatggag ctgttgtaat tagagattct aaggtaaaag cagctgcatg
     1561 ttttttgcca ttgacagaaa gtaaggattt aagtaaagat ttgggcacta gacatagagc
     1621 aggaataggg gtaagtgagg tgtctgactg tataactttg attgtatcag aggaaacagg
     1681 tggcgtatca attgctaaag caggtaagtt atatagagat atttctagag aaagaatgat
     1741 gaatatatta cgtagtaatt taaaaacaaa tacagaaaca agaagttttt tcaaaggtgg
     1801 tatatttaaa tgataaatag actaaaaaat aataccaaaa taaaaataat atcattttta
     1861 agtgcaattg cattatggat gtatgttatg gctgtagttg accctgaaga aactaaaggg
     1921 tttgaagata tacctgtatc tataagtaat atgagtgatt taaaggataa aaatcttgtt
     1981 atatatccag acgaaaaatt aacagcagat gtatatataa aaggtagttt atcaaaggta
     2041 caaagtgtta aaaaagaaga tatacatgta tatggaacca ttgaagaccc aatacaagga
     2101 cagaaggaag tatatttaaa agcaaatatt cctcaaggtg ttactctaga atttaagcat
     2161 gatgttttag tagtaaatct tgaaaaaaac ataaaagaaa aaagagatat aaaggtagaa
     2221 gtaagtggaa attcaaaaag aaatataaat aaaattgaac ttagtaagga atttgtcgat
     2281 gtatcaggtc caagaactct agttaaagaa gtcaaaagtg taagagctaa tctagatgtt
     2341 ggtaatgaga cagatgattt ttctaaaaaa ttgcaattaa taccattaaa tagtgatgga
     2401 actaaggttg aaggagtaac attaagttca tcctttgtga ctgcaaagat agagctattg
     2461 aaggaaaaaa ttgtcccaat aagagtaagt ctaaatgata ataaagataa tgatgtactg
     2521 aaaaactata aattaagtaa ggaacaagtt acaataaaag gaaagaaaga agatatagac
     2581 aaaataagtt atattaagac aaaacctatt gacttagctg atttggctaa tggtgaagaa
     2641 aaagaagtta gcttagaaat tccagagggt ataactcttg aggatagctc tataacaatt
     2701 aaattaaatg atacaaaaca attgagtgca gaatttttat ataatgcggg agatattgaa
     2761 ttaagaaata tcccaacaaa tatggataat ccaaatgtaa tttctgatgt agatataaaa
     2821 gttgttgtag agtcaacgga ggatttgagt aagttaaata aaaaagatat ttcattatat
     2881 attgatttat cacaaggccc tgaggaagat agtaaatata aaataaaata tgaaacaaca
     2941 tatcaattta aaaaaataaa tatagaacct aatattgttg aagtggaatg aacaaatatt
     3001 tcgaatattt agttgttaca agatgcgaca aaaaaaccta taaaattaag tttgttaatt
     3061 ttatgttttt gttgcatctt gtttatttta gttatattat aaaataagga caatgagata
     3121 tttgttaaat ctaaaaagcg ctagatatat gaaaaaagac tgataaatag atttaaataa
     3181 atattttgaa ttaaatattt caattatctc taggaaaacg ttgctgaacg agttataatt
     3241 taattgagat ataaaaagtt ctatttcaaa aaattcaaga tgaggtggaa atgcatgaga
     3301 agttttgaag aagtaattaa gtttgcaaaa gaaagaggac ctaaaactat atcagtagca
     3361 tgttgccaag ataaagaagt tttaatggca gttgaaatgg ctagaaaaga aaaaatagca
     3421 aatgccattt tagtaggaga tatagaaaag actaaagaaa ttgcaaaaag catagacatg
     3481 gatatcgaaa attatgaact gatagatata aaagatttag cagaagcatc tctaaaatct
     3541 gttgaattag tttcacaagg aaaagccgac atggtaatga aaggcttagt agacacatca
     3601 ataatactaa aagcagtttt aaataaagaa gtaggtctta gaactggaaa tgtattaagt
     3661 cacgtagcag tatttgatgt agagggatat gatagattat ttttcgtaac tgacgcagct
     3721 atgaacttag ctcctgatac aaatactaaa aagcaaatca tagaaaatgc ttgcacagta
     3781 gcacattcat tagatataag tgaaccaaaa gttgctgcaa tatgcgcaaa agaaaaagta
     3841 aatccaaaaa tgaaagatac agttgaagct aaagaactag aagaaatgta tgaaagagga
     3901 gaaatcaaag gttgtatggt tggtggacct tttgcaattg ataatgcagt atctttagaa
     3961 gcagctaaac ataaaggtat aaatcatcct gtagcaggaa gagctgatat attattagcc
     4021 ccagatattg aaggtggtaa catattatat aaagctttgg tattcttctc aaaatcaaaa
     4081 aatgcaggag ttatagttgg ggctaaagca ccaataatat taacttctag agcagacagt
     4141 gaagaaacta aactaaactc aatagcttta ggcgttttaa tggcagcaaa ggcataattt
     4201 taagggggaa ataggcaaat gagcaaaata tttaaaatct taacaataaa tcctggttcg
     4261 acatcaacta aaatagctgt atttgataat gaggatttag tatttgaaaa aactttaaga
     4321 cattcttcag aagaaatagg aaaatatgag aaggtgtctg accaatttga atttcgtaaa
     4381 caagtaatag aagaagctct aaaagaaggt ggagtaaaaa catctgaatt agatgctgta
     4441 gtaggtagag gaggacttct taaacctata aaaggtggta cttattcagt aagtgctgct
     4501 atgattgaag atttaaaagt gggagtttta ggagaacacg cttcaaacct aggtggaata
     4561 atagcaaaac aaataggtga agaagtaaat gttccttcat acatagtaga ccctgttgtt
     4621 gtagatgaat tagaagatgt tgctagaatt tctggtatgc ctgaaataag tagagcaagt
     4681 gtagtacatg ctttaaatca aaaggcaata gcaagaagat atgctagaga aataaacaag
     4741 aaatatgaag atataaatct tatagttgca cacatgggtg gaggagtttc tgttggagct
     4801 cataaaaatg gtaaaatagt agatgttgca aacgcattag atggagaagg acctttctct
     4861 ccagaaagaa gtggtggact accagtaggt gcattagtaa aaatgtgctt tagtggaaaa
     4921 tatactcaag atgaaattaa aaagaaaata aaaggtaatg gcggattagt tgcatactta
     4981 aacactaatg atgctagaga agttgaagaa agaattgaag ctggtgatga aaaagctaaa
     5041 ttagtatatg aagctatggc atatcaaatc tctaaagaaa taggagctag tgctgcagtt
     5101 cttaagggag atgtaaaagc aatattatta actggtggaa tcgcatattc aaaaatgttt
     5161 acagaaatga ttgcagatag agttaaattt atagcagatg taaaagttta tccaggtgaa
     5221 gatgaaatga ttgcattagc tcaaggtgga cttagagttt taactggtga agaagaggct
     5281 caagtttatg ataactaatg ataaaagaat aagaatcata actggtcact atggaagtgg
     5341 taaaagtgaa tttgcgatga actatgttgt taagttaaga gatatggttt ctggcaaagt
     5401 tgctatagcc gatttagatg tggtaaatgt atactttaga actagagaaa agaaagagtt
     5461 aatgaaatcc ttaggtatac aaccaataga tagctcaatt aatgctccaa cattagattt
     5521 accagcagta tcagcagagg taatgtctcc tatggtagac cattcataca attctgttat
     5581 agatttaggt ggagataatg taggtgctag ggttatagga agatttagtc atttgttaaa
     5641 agaaggcgat tatgatatgc tttttgtaat aaatgcaaat agggagaaaa ctcaaacttc
     5701 tgaagaagtt atccaatata taaaagaaat agaaaaatct tctaaattaa aggtaacagg
     5761 tctaattaat aatactcatc ttattaggtt tactactatt gatgatgttt taagaggtca
     5821 gaaagttgca aaagaagttt ctgaaaaatg taatataccg ataagatatg ttgcatgttt
     5881 agaaaatctt gttgaacagt taccaaaaga tttagaagga gaaatttttc ctataaaatt
     5941 atatatgaga gaagactgga tgtagtttga gctcaaatat atagaaagtt taaggaggct
     6001 taggtaaatg gctaaaggaa aagtatcttt taatcaagaa cgttgtaaag gttgtggatt
     6061 gtgtgttgaa gcatgtccag taaaaatcat acaattagac tcaaatgtaa taaacaaaaa
     6121 agggtataac ccagcaactg tttttgaaat ggaaaaatgc ataggatgtg caagttgtgc
     6181 aactatgtgt ccagatgtag ttataacagt agaaagagat taatttaatt aatatttaag
     6241 ggggaatttt aatggctaag atacttatga aaggtaacga agcatttgga aaagcagcta
     6301 tagaagctgg ttgcaaatat ttcttcggtt acccaataac tccacaaagt gaattaccag
     6361 aatatttatc aagagagtta cctaaaatag gtggagcgtt cgttcaagct gaatcagaag
     6421 tttctgcgat aaacatggtt tatggtggag caggtgcagg agcaagagtt atgacttctt
     6481 catcttcacc aggagttgct ttaaagcaag aaggtataac atatgcagta ggagcagaag
     6541 ttccttgtgt tgtacttaat gtaatgagag gtggtccagg acttggaagt atacaacctt
     6601 cacaagctga ctactttatg tctacaagag gtggaggaaa tggagactac agaactccag
     6661 tattcgcacc agctacagtt caagaagctg ttgatatgat aatggaagct tttgacgttg
     6721 cagattatta cagatcacca gttatggtag ttgcagatgg tatgataggt caaatgatgg
     6781 agccagttga atttagagct cctgagaaga aaagagaatt gcctcctaaa gattgggcaa
     6841 ctgttggaac taaaggaaaa agaaaaccta atgtaataaa ctctctatac ttagagccag
     6901 aagtactaga agaccattgt tggcatttac aagaaaaatt tgatgcaatg gaaaagaatg
     6961 aagtacaata cgaaatgtac aaaacagaag atgctgaatt tgtatttgca gcttatggaa
     7021 ctacttcaag agtagtaaaa agcgcaattg atatattaag agaagaagga ataaaagcag
     7081 gtcttataag acctaaagta ttatggccat tcccattcga agcattcaac caaattccta
     7141 atgctagaaa catattgact gttgaaatga gtatgggaca aatggtggaa gatgttaaaa
     7201 tggcagtaga aggtaaatta ccagtttact tccatggaag accaggagga atgactccaa
     7261 caccagcaga aatagttgaa aaagctaaaa aaattattgc cggagaattg gttgcaggag
     7321 gtgctagata atggctgttg tatttaagaa aactgaagga ttacaagata ctcaaactca
     7381 ctactgtcca ggatgtacac atggtataat ccatagatta gttggtgaag tattagaaga
     7441 actaggagta ttaggtgatg ctgtaggtgt tgttccagtt ggatgttcag ttttaggata
     7501 taaatatttt aactgtgaca ctcaagaagc tgctcatggt agagcgccag ctgctgctac
     7561 aggaataaaa agagttcatc ctgaaaatac agtattcact tatcaaggtg atggagactt
     7621 agcttcaatt ggtactgctg aaatagttca tgcagctgct agaggagaaa aaataactac
     7681 tatatttgta aacaatacaa cttatggtat gactggtgga caaatggctc caactacatt
     7741 agttggacaa agagctacta ctgctcaatc aggaagaaat gctgaaactc aaggttatcc
     7801 aataagagta agtgaaatgt tagctacatt aacaggagct gtatttgtag aaagagtagc
     7861 agttgatact ccagctcatg ttagacaagc taaaaaagct ataaagaaag cattccaagt
     7921 tcaacaagca ggattaggat ttggaatagt agaagtatta tctacttgtc ctacaaactg
     7981 gggactagct ccaaacgatg ctttacaatg gttaagagac aacatgatac catactatcc
     8041 attaggaaat ttcaaaaatg ttgaagttga ggaggtgaag taaatgtcta cagctagagt
     8101 aatatgtgca ggcttcggag gccaaggtgt tatgtctatg ggacaattac ttacttatgc
     8161 aggaatgcta gaaggaaaag aagtttcttg gttaccatct tatggaccag aaatgcgtgg
     8221 aggtacagca aactgtgcag taactgtatc taatgagcca gttggttctc cacttataac
     8281 agatgatgca actgcagcta tattattaaa tataccagca tttgaaaaat tcaaagatga
     8341 tgttattcct ggtggaaaaa taatagtaaa tagttcttta ataaaagaaa aagttgatag
     8401 aacagatgta gatgtttatt atataccagc aaatgaatta gcagctgaat taggaaatga
     8461 taaagttgct aacatgataa tgttaggcgc ttacttaaaa gtatctgata ctgtagatat
     8521 agaatctgta ctagaagcat tcaaaaaagt tttcggacct agaaaagaaa aattcgttcc
     8581 attaaacaga gaagctttac aaaaaggtat ggatgctaca tgttctaaag caactaatta
     8641 ataaagaaaa agatgtcaaa aaaaagacaa tagaattatt tctattgtct tttttgctga
     8701 taagtattaa aatattttta tgactgaaat taagcctata atgatatttt atgaactata
     8761 aaaatataat acaaattaca agcttttcaa ttatgaaagg agaatcttta gtgagaaagt
     8821 attttggaac agatggagtt agaggagtag caaatacaga actaacatgt gatttagcat
     8881 ataaacttgg tagagctggt ggatttgtct tagctcaagg agaccacaga gtaaaagtag
     8941 tagtaggtaa ggatactaga atatcaggtg atatgttgga agcatcttta atagcaggac
     9001 ttatgtctgt tggatgtgat gttattactg ttggaataat accaacacct gcagtggcat
     9061 atttaactag aaaatatggt gctgattgtg gtgtggttat atctgcatct cacaatcctg
     9121 ttgaatataa tggaatcaaa ttctttaata agaatggata taaattagat gatgaaattg
     9181 aattgaaaat agaagaatat atagacgata tagacaaaat tgattgtctt ccaattggtg
     9241 aaaatgtagg aagaaaatta catgaacatt gtgcacaaag agactatgta gactacctaa
     9301 aatcaataat aagcactgac tttaagggat taaaagtagt tttggactgt gctaatggtg
     9361 cgtcatataa agtggcacca atagtgtttg atgaactagg agcaagtgta atttcaataa
     9421 atagttcacc agatggaaat aatataaact ataaatgtgg ctctacacat ccagaacaac
     9481 ttcaaagggc tgttttggaa catgatgctg acttaggtct tgcatatgat ggagatgctg
     9541 atagattaat tgcagtaaat gaaaagggtc aaattgttga tggagaccat ataatgatat
     9601 tgagtgcctt aaatttaaag aaaaataata aattggcaca agatactttg gtagttacag
     9661 ttatgagtaa tatagggttg acaatagctg ctaaggaaaa tggaataaat ttatctacta
     9721 cagcagttgg agacagatat gttttagaag atatggttaa aaatggatat aatcttggtg
     9781 gagagcaatc tggacatatg atatttttag attacaacac tactggagat ggagtgctta
     9841 gttctctaat acttgcaaat ataatcttgc aagaaaagaa acctttatca gaaattgcat
     9901 caataatgag ccaataccca caagtattag taaatgcaac aataaaaaat gaaaataaga
     9961 acaaatatat ggaatatcca gaaataaaaa ctgagataga aagaatagaa tctattttag
    10021 acggaaatgg tagagttttg ataagaccat caggaactga gccattagtt agagttatgt
    10081 tagagggtaa agaggaaggt caaataaaag aattagcaac aaacttagct aatcttatac
    10141 aagaaaaatt atcgtaattt aaagggtatt ctcagatata aatactgagg acacctttct
    10201 aagcgccaga acttagttat agacataatt attgagtaat ataggaacta agttgacgag
    10261 gtcagagtta atcgaattgt cggcggatgc tctgcggtgt gtttaccatc gaaagacttc
    10321 tttaaataat aggagcaatc ctatggacaa agggaagcca aagtaaactt atatgaccta
    10381 actcttaaaa tatataaata atatttttag gaggacacaa ataatgtgtg gaatagttgg
    10441 atatttagga agtagaaagg cagcagaagt tatagtagaa gggctttcta agctagagta
    10501 cagaggttat gactcagcag gagtagcagt taatagttct aatgaaaaag aacttaatat
    10561 tagaaagttt aaaggtagac tttctgtact tgcagaagac ttagaaaaga atcctattga
    10621 tggaaattta ggtatagggc atacgagatg ggcaactcat ggagaaccat ctgatgtaaa
    10681 ctcacatcct cattttaatc aagccaaaac aatagcagtt gttcataatg ggataataga
    10741 aaactatatg gaaataaaag aagaattaat ttctgaaggt gttaagtttg aatctcaaac
    10801 agatacagag gtaatagcac atttagtaga taaatactat gagggtaatt tattagatgc
    10861 tgtatacaaa actatatcaa aattaagagg agcttatgca ttaggtgtta tatgtaaaga
    10921 acatggaaat gaattagttg cagtaagaaa agatagtcct cttgtagttg gtgttggtga
    10981 aggtgaaaac tttatagcat cagatatacc tgcattatta aaatatacta gagatgttta
    11041 tttcttagaa aatggagaag tagttcatct aaaagacgaa aatgtaacag tttatgactc
    11101 taatagaaat cttgttgaaa aagaagtatt tcatgtaact tgggatgtag aagctgcgtc
    11161 taaaggtgga tatgactact ttatgtcaaa agaaattcat gagcaaccaa caggagtaag
    11221 agaaacttta gaaagaagac ttgatgataa tggaaatata attctagaca gtataaatat
    11281 ttctaaagaa gacttagaaa aaataaataa agtttatata gtggcttgtg gaacagcata
    11341 taatgctgga ttacttggta agtatgccat agaaaaattt gtaaatatac cagtaataac
    11401 tgatatagca tcagaattta gatatagcga cccatttgta gatgaaaatt cattagtaat
    11461 attagttagt caatctggtg agacagcaga tactctagca gtattaagag attcaaaggc
    11521 taaaggagca agaatacttt caataactaa cgttgtaggt tcatctatag caagagaatc
    11581 agatgatgta ttctatactt gggcaggacc tgaagtagca gttgcatcaa ctaaggcata
    11641 tactactcaa ataacttctt tatacatgat agctttagac tttgctatca aaaaaggaac
    11701 aataactaga gaattttatg atagtatgat ttctaagatg aaagaaatac catcaaaaat
    11761 acaagaaata ctagacaatg aagaatatat aaaagaagtt gctaaaacag ttgttagttc
    11821 tgaacatgca ttctatttag gaagaggtat agactatagc ttagcaatgg aaggttcatt
    11881 gaaattaaaa gagatttcat atatacatgc agaagctttt gcagcaggag agctaaagca
    11941 tggaacaata gctttaatag aaaaaggaac tcctgtaatt gccatagcta ctcaagaaaa
    12001 gttatttgaa aaaatggttt ctaatatgga agaagtaaga gcaagaggag cttatgtagt
    12061 agcaatagct caaagccata ataaggatgt tgagaaagct gctgataaaa taatctatat
    12121 accaaattca gatgatattt tatcaccaat attagcagtt gtaccaatgc aattacttgc
    12181 ttatcatgtc tctgtactaa gaggatgcga tgtagataag ccaagaaatc tagctaaatc
    12241 agtaacagtt gaataaaaac agattaaaaa ttaaatagat aatatttatt gaaaagaact
    12301 catcctgtta tttgtatttt ataggatgag ttttttagat aaatattaaa tttgttttgt
    12361 ttttgctata ataaatttta taaaaagatt gatattaaat catctgtaaa atgataaata
    12421 tatagaccat accaggtata gggaacgata aatatagttc atataaaata tagtgagtac
    12481 ttaataaaca tatagtcaat atcagataaa aaaataatag cttatgagaa aaattaaaat
    12541 acaaggtatt agtaaccttg tgataagata aagtgagtag tttaaaataa gaatataagg
    12601 aactatttat aaaagaatat aatgaagcta atagagtata agaaaattac tttattttag
    12661 taaaataatt tcaaataaaa atttttttaa attgggagta taaattatga caaatgattt
    12721 taaaaaagaa aagaaagaag attattttgt taatcttaag gcaatatcta ctgaagtttt
    12781 acaagaaaaa gtgcaagata acgcttgggt tatagttgat actcgcttaa atgatgctta
    12841 taatggctgg aagttagatg gagtaaaaag aggtggacat ataaaaggag cagtagattt
    12901 ttctgctaat tggttaagtg tatactcaga tagaaaagat gaagtgttag aacaagcatt
    12961 gaaaactaaa agaattgatt tagataaaaa tatagtattg tatgatgcaa atggaaaaga
    13021 tgctttggtt gtagcagatt atttatctaa aaaaggatat aaatatctat ataaatatga
    13081 tattaaacaa tgggcagacg acgaaaatct tccaatggaa agatataaaa attatcaaat
    13141 gatagttcct gcttttataa taaaagatat attagatgga aaaattcctg agacatttga
    13201 agattcaaag aatataaaaa tgattgaagc gagttgggga gaagaatcgt atacaaaagg
    13261 gcatatacct acaagtgttc atgtaaatac tgatattata gaacctcctc caacatggat
    13321 gcttgataat gatgataact taactaagtt tgctttagac tatggattaa caaaggatga
    13381 tactgtaata gtttcaagta gtacacctat ggcatcttat agactagctg ttatattgag
    13441 atatatagga gtaaaagatg taagagtttt gaatggtgga acaaactcat ggttatcagc
    13501 aggatatgaa ttagaattta ttagtaatcc aaaacattca tgtactaatt ttggagcaga
    13561 tataccagtt aactcacaat taattgttac tacatctgaa ttaagacaaa aactaaaaga
    13621 aaaaaataag tttatactag tagataaccg tacttgggat gaacatatag gaaaagtatc
    13681 tggatataca tattatgata aaaaagggcg tataccagga gctttatatg gacattcagg
    13741 tagtgatagt gtttcactag aagaatatcg taatattgat aatacaatga gaaataaata
    13801 tgagatttta gaaatgtggg acaaggaaaa tatagatgtg aataaacagc ttatatttat
    13861 gtgtggaagt ggatggcgtg ctgctgaagt attaacatat gcaaatgtca taggagtgga
    13921 aaatacctcc ctttatagtg atggatggat gggctggagt cttgataatt caaatttaat
    13981 tgaagtggga gaacacaaat aaagtttttg aaagtgcaat agagttgttt aaaacaaagg
    14041 tgtttttgat ttattaggaa gagtgtgtaa attgtgtatt gggaagtgta cttaggtatg
    14101 ttcatttgga gctatggaaa ttgataaaaa agtattatct gtttattttg ttggtaaaat
    14161 atgaatagat tatagattat agattataga ttatagatta tagattatag atttaagtat
    14221 agactaagta atttatatta cagaagagaa tatataagaa ttaatgcaag atgtatttga
    14281 tacatataag aaattggcaa gtccaggaga aggatagaga aaattgagat tgaagagttt
    14341 gaaagagatt attagatgaa aaatacaaaa atgtataaaa atacatattg agaaagtata
    14401 tctctcttaa atttgttaat aattctatta tacagaaaaa catatttaag ggggattttt
    14461 tactatgaaa aaagtaaaat tagctataag cttttttata ttaatgatta taggaatgac
    14521 aatatcttat gcagatacaa gtataaatac tagatacaat agattaagta aagctcttga
    14581 aatatcagaa gcaaatttta aattttataa tgttaaaatt aattctgtta taaatcataa
    14641 tttatcaaat aaagaatttc ataatatttt tatagaaata attaacaatc taaagttgga
    14701 taaaaaagga ataaattgga atcaaaaagg gtctggcgat aaaatacaat tatgtgctac
    14761 tataaaaggt agcgataaag aaatttctat aaaaggaata aataaaaata acaaagaaag
    14821 ttacatagta gttgacatat tggataacaa agtatataaa cataaagagg ttatttatag
    14881 taacgtagaa aatgtgttaa ataaatattc atctcaagta gatgtgtctg cttgtattgt
    14941 tggagaatac acaaaaaggt tacaaaacaa caaatatgat gatattttac aaaaaatatt
    15001 atataatatg agtgctaagc aaatagatga agttaaagac gtaaactttt tatctataac
    15061 agcatatagt aaagtattaa atgattatga attagaatat ttaggggaaa aaattaattt
    15121 aaatatagga attaaatata atgaggatga cggaaaaaca ctcatatata ttgccacacc
    15181 aattataaaa ttagattatt aattatgagg agatcaaaaa atatggctaa gataatagta
    15241 aaaaagagta atccacttaa aggtagtgtt aaaatagatg gtgcaaaaaa tgcagtttta
    15301 ccaataatag cagcgacttt attagcaaac ggaaaatcaa cattaaatgg agtaccaaat
    15361 ttaagagatg ttcatgttat atcagattta ttaagacatg taggagcaga agttgaatat
    15421 aaagaaaata cacttactgt tgatgcaagc aatataaaaa cttgtgaagc tccatatgag
    15481 cttgtaagaa agatgagagc ttcttttttg gttatgggac cattgcttgc tagatttaat
    15541 agtacgaaaa tttctatgcc aggaggatgt gctataggaa caagacctat agatttacat
    15601 ttaaaaggat ttaaagcctt aggtgcaaag atagaaatgg accatggttt tgttgaagca
    15661 gcaacagaaa agttagtagg aaataaatta tatttagatt tcccatctgt tggagcaact
    15721 gaaaacataa tgatggcagc ttctttagca gaagggacta caataataga gaatgcagca
    15781 gaagagccag aaatagtaga tttagcaaac ttcttgaacg aaatgggagc agatgttaaa
    15841 ggtgcaggta ctaatactat aaaaataaaa ggtgttaaag agttaaaagg agcagaacat
    15901 aatgtaattc cagacagaat agaagcagca acttatatgg ttgcagcagc tatgacaaaa
    15961 ggtgatataa ctgttgaaaa tgttttaatg gagcatttaa aaccagttgt agctaaatta
    16021 agagaagctg gttgtgaaat aacagaaatg gacaattctg taagagttgt aggtccaaaa
    16081 gtattaaaac caatagatat aaaaacttta ccacatccag gatttcctac agatgttcaa
    16141 gcacaattta tggctatgct tactgtagcc aatggaactg gtgtagttat agaaactgtt
    16201 tttgaaaata gatttatgca tgttgctgaa tttaatagaa tgggtgctaa tataaaaata
    16261 gatggaagaa gtgcggttgt taatggtgta gatgagctac acggtgcagc agtaaacgct
    16321 actgatttaa gagcaggggc agcccttatt ttatgcggtc ttatagcaga aggagaaact
    16381 caaataggag aaatatacca tatccaaaga ggttatgttg atatagataa gaagataaca
    16441 gcactaggtg gtcaaatcga aatagtagaa gattaataat tggtttatag aagaaagtta
    16501 taaattaata gggttgttca tatacttaaa tacaaggttt taaaagctaa ggaggagagt
    16561 atatgaagaa cccattggtt gttttgttag gatttgtgac ttgctctgtg ttagttccat
    16621 cacttataac tttagtttct tataaaaatg tagaattaac tgaaaaacca gagagcccag
    16681 tatctataaa caaaactatt aaaaaaagtg atattgaaga caaaggtaat aaagaagaaa
    16741 aaaatacagt gaactatgaa actgtaaata aaaaagcacc aattatcaat gtatataatc
    16801 atataacagg gaaaaccgaa aagatggaca tggagaacta tttatgtgga gtactagctg
    16861 gtgagatgtc ttcagagttt gatatagaag ctttaaaagc tcaatctgta gcagctagaa
    16921 catatgtagt atataaacag gaacatggta aatctagtaa acataaaaat gcagtagtat
    16981 gtactgatta taaacattgt caagaatata agagttatga tactcttaaa aaattaaatg
    17041 gtgaagaatg gattaagaat aaatattcca aaatccaaga agcagttaga ggaactaaag
    17101 gtcaaataat aacttataat gataaggcga tacttcctct ttatttttct acatcttcag
    17161 gaaaaacaga aaatagtgaa gaagtatttt ctgcaaaata tccttattta aagtctgtgg
    17221 aaagtcctta tgataagtat tcccctaaat ttgcgtcaac acttaaaata agtaacacag
    17281 attttgttaa aagtcttaga agggcatata gcactattgt gatagatgtg aataatttaa
    17341 gtaaacaagt ttcaataact aaaagaagtg atgccggaac tgtagaaaaa ataaaattag
    17401 gaaataaaga attgactgga aaagacatta gaactgtttt taaattgaac tctgcaaact
    17461 ttgacataaa atttggagaa ggatacatag atttcgtggt aaaaggatat ggtcatggtg
    17521 ttggtatgag ccagtgggga gcagaaggaa tggcagaaga gggctataaa tattatgata
    17581 tattatcaca ttactacaca gatacaaaaa taaaagatat atactaataa ttgtataaaa
    17641 tgccaaaaac acgtcaataa tccaaatgta gaaatatatt tggaggtgca aaatgaagaa
    17701 aaagctgtta gaaaaagatg gtttttattt atctttgttt gtatgtgttt gtttattagc
    17761 agtaggtgga gtttggttta caaataataa tgtagataaa ttggcttcta ataaaggaat
    17821 tatggaaaat gctaataaag atagtgaaga ggaaatacat ttaattgaaa aagacaagaa
    17881 agatgctatc cctactgcaa cggattcaaa acaaaactta gaaaaagcta agtcaaaaga
    17941 ggaaaataaa tcgagtacaa caaaattaaa ttacattgga gataaagtaa taagaggata
    18001 ttcagaaaaa gagcctagtt attctaaaac acttgatgtt tgggaaactc ataaaggtgt
    18061 agatattagt tgtactaaag gcaaagaagt aaaatctcta ttaaacggaa tagtagtaga
    18121 tgtatttgat gatgaagaat atggacaatc agtcaaaata aaaagtgaca ataacattgt
    18181 agtagtatat tcaaatctag ataaaaatgt tagtgttaag aaagaacaga aggtaacaga
    18241 aggacaatct ctaggtactg taggtagtac ttctcaaata gaaagtgaag aaggtataca
    18301 tgtgcattta gaggcgtata gtggagaaaa atctatagac ccaatgagtc taattaagta
    18361 atagtataac aagtttaatc atatttggtt aagcttgtta tattttttgg tgtttttaca
    18421 tatatatcat taaagctatt atgtgagggg ggtaatcttt gagatctcat atagaggaaa
    18481 gggccatagt ggtagcaaaa tatatacttg aaaaaaacac tacagtaaga caaaccgcca
    18541 agacatttgg agtaagtaaa agtacaattc ataaggatgt tacagaaaga ttgaaggaaa
    18601 taaatccatc ccttgccaaa gaagttaaaa atgtgctgga caaaaataaa tcagagagac
    18661 atattagagg gggattagca acgaaattaa aatatgagaa ggaacataaa aaaatgtagg
    18721 ttaaaaacct acttttttta ttgaattaaa ttataaa
//
LOCUS       DAEORZ010000076        18712 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEORZ010000076 DAEORZ010000000
VERSION     DAEORZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.076"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            256..969
                     /locus_tag="KRH39_002309"
     CDS             256..969
                     /locus_tag="KRH39_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBG8512208.1"
                     /translation="MNKISKRGFDDGDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEETLNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            1087..1956
                     /locus_tag="KRH39_002310"
     CDS             1087..1956
                     /locus_tag="KRH39_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG8512209.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLKVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(2111..3256)
                     /locus_tag="KRH39_002311"
     CDS             complement(2111..3256)
                     /locus_tag="KRH39_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG8512210.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(3589..4365)
                     /locus_tag="KRH39_002312"
     CDS             complement(3589..4365)
                     /locus_tag="KRH39_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG8512211.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            4947..5921
                     /locus_tag="KRH39_002313"
     CDS             4947..5921
                     /locus_tag="KRH39_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG8512212.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            6019..6324
                     /locus_tag="KRH39_002314"
     CDS             6019..6324
                     /locus_tag="KRH39_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG8512213.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            6480..7640
                     /locus_tag="KRH39_002315"
     CDS             6480..7640
                     /locus_tag="KRH39_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG8512214.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            7690..8592
                     /locus_tag="KRH39_002316"
     CDS             7690..8592
                     /locus_tag="KRH39_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG8512215.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            8672..9565
                     /locus_tag="KRH39_002317"
     CDS             8672..9565
                     /locus_tag="KRH39_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBG8512216.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      9618..9799
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10027..11157
                     /locus_tag="KRH39_002318"
     CDS             10027..11157
                     /locus_tag="KRH39_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8512217.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            11229..12254
                     /locus_tag="KRH39_002319"
     CDS             11229..12254
                     /locus_tag="KRH39_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG8512218.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            12257..13453
                     /locus_tag="KRH39_002320"
     CDS             12257..13453
                     /locus_tag="KRH39_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512219.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(13581..14306)
                     /locus_tag="KRH39_002321"
     CDS             complement(13581..14306)
                     /locus_tag="KRH39_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG8512220.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENIDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEYATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            14784..16883
                     /gene="nrdE"
                     /locus_tag="KRH39_002322"
     CDS             14784..16883
                     /gene="nrdE"
                     /locus_tag="KRH39_002322"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBG8512221.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLKKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGNKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIETTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            16896..17867
                     /gene="nrdF"
                     /locus_tag="KRH39_002323"
     CDS             16896..17867
                     /gene="nrdF"
                     /locus_tag="KRH39_002323"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBG8512222.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            17943..18569
                     /locus_tag="KRH39_002324"
     CDS             17943..18569
                     /locus_tag="KRH39_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG8512223.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGEPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         7168 a         2108 c         3289 g         6147 t
ORIGIN      
        1 gattttataa aagaagatta ttttatgagc taaaaagttt atttagaaac actacctatt
       61 tatgttataa agttataaat ataatataga atctattatt ctgtattatt aagtggggtt
      121 aagtgttata attaattata aatattgaat aatctaaatg gataagataa tggtattaaa
      181 aattatctta aaaggtgaaa taatctgtat ttaatgtagt acagttatat ctttaaagca
      241 aggtggtgaa atgatataaa taagatatct aaaagagggt ttgatgatgg tgataaaaga
      301 tggttttcaa gtaaagaatt aacaagactt ataaaggcta aagaagaaat agaatggtta
      361 ttaaataggg attacaaaat agacccagtc gttacttttg ttggtgatag atatcagttt
      421 tctataagac aaagagatgc attaaaaagg gcagtctgta ctgatgaaaa aaatattata
      481 agacaatcaa aaaggctttc tctagataaa atcaaagaag ataccgtaaa tatagatggc
      541 tttaatttga taatatccat agaggtagca ttatctggtg gtactctagt aataggaagt
      601 gatggcaata taagagattt agcaggtctt aggggaacct ataagattat agataaaaca
      661 gaagaaactt tgaatctgat aggtaaattt tttaataaat acaatgcaca gaatcttaaa
      721 ttttatttag atgctccagt ttctaattca ggaaatttaa agtataggat attagagcat
      781 gccaaaactt ggggaattga aactgaagta gaattagtaa aaaatgcaga tgttgtattg
      841 gaaaaattag atagagtagt tagtagtgat gcagtcatag ttgacaagtg tataagttat
      901 tttaatgtgg ctagaggtat aattgaagag tatattaaag aatgtaatat cataaattta
      961 aataaataga taaatatagg tgagaatata taagtatatg tagcaatatt attagtaata
     1021 caagtaaagt ttgaaaatag gagaacgaat acatatcgaa tttcataaaa tgttgtaaaa
     1081 agacatatga gaggatggat atgtgtgaaa ttaactaaac taaatattaa aaaatataga
     1141 gcacctatat ataaatatgc tttagcaaat gtaaatttga ggtctgctaa atctaccaat
     1201 tcaagtatca taacagtaat acctcaggga gccaaaatgg aagtattgga tgaagaagat
     1261 gactggatta aggtgatgta caattctcaa gaaggatatg tgtataagga tttggtatca
     1321 gtaagcgaat atgcttggag taacttaaac ttaagagaag ataaatctac aacttccaat
     1381 ataattacag taattcctga aaaatctagg gttgaagtcc ttaaagtaga tggagattgg
     1441 agtaaggttg tatatgatga taaaatagga tatgttttta attatttttt atcaattgat
     1501 gggaataaac caaatgaatt agattataag tatttttata ctgacatgat taaatttgtt
     1561 aacgaaaata atattaaaag tactagcgat tatctaattg taacagacct tagaaataaa
     1621 tatacttata tttttaagaa agataatgga ggatggggac aactttacaa gtggcaatgt
     1681 actataggca agcctgagac tccaactata acaggtatat tttatataag tggaagaaaa
     1741 ccttcttttg gaacggatga gtattcagtt aagtatgcta ctagaattaa aggtggatat
     1801 tactatcatt ctgtactata tgattcaaca ggaagttata taatagatgg cagacttggt
     1861 gaagctctta gtcatggatg tataagatta agtacggaaa atgcaaagtg gatatatgat
     1921 aatataccag atacaacaac agttataatt cattaagaaa aattttgagt tttttattgg
     1981 gataatgatt cttaaattaa taggattata tattaacttc tcataaagag tactatttaa
     2041 aaagtgtaat aagagagtgc tattaacaaa acaaagatat tttgaggcac tctcttataa
     2101 atatacataa ttaagatatt ttcataaaca attctataat cttatcttta tcaaagaaaa
     2161 caggattagc accagcacaa gcatctttca tagagttttc tgcaagcaat tcaaaattag
     2221 gattatcaac acctacatct tttaaagatt ttgggattcc aacctcttca gaaagagctt
     2281 ttatcttatc tattacaaag tcaacacact ctttatctga tttattattt acatccattc
     2341 caataacttc agctattggt cggaatttgg caggggctga tttagcattt tcctcctcaa
     2401 ctattggaag tagcatggca ttacatactc catgaggtaa atcatataaa cctcctaatt
     2461 gatgagccat agcatgtaca tttccaagac ctgcattact aaaggcaatt ccatttaaaa
     2521 aacaagcata acacatttgt tctctagctt ctatgtcatt accattttta acagctcttg
     2581 gaaggtattt aaatatctgc tttatagcat ataaggcagt tgaatctgta acatcataag
     2641 caccattagc tactacagct tctatagcat gagttagtgc atccatacca gttgctgctg
     2701 ttaaattaga tggtttgcta atcattaatt ctgggtcatt tactgtcatt gtggctagtg
     2761 aattggtatc aaccattatc atctttacat gtctgtcttc atcagttata acatagttta
     2821 ttgtaacttc agcagatgta ccagcagtag tagttatagc tatgatagga agacattttt
     2881 tagaagtttt gttgatacct tcatagtctc taatatctcc tccattagtg gctaatatag
     2941 agatagcttt accacagtct tgtggagaac cacctcctat agttataacg aagtcgcaat
     3001 tttctgattt taatatttta agtcctttat tcacattagt tacagttgga ttttgtttaa
     3061 cttcatcata aacaacaaag tctatatcta tctgagataa aatatctgtt acttttttta
     3121 cagtaccatt tgaaactaga aatttatcac taacaataaa tgctttttta gttcctaatg
     3181 atttaattga atctttagct tcattaagac atcctttccc aagtaaattt attggtggta
     3241 cataataaat gctcataact aaaatctcct tttattttca ttgattttta tgtaaaaata
     3301 tatatctaac aattttcact aatttactca taatatatcg tatcttagat tgagataggg
     3361 ttgctggttc ttatataaga acttaattcg taattacgat tatgattaga atttaacata
     3421 gtatttttat catgtcaata aaaatataaa aaatattata aaaaaaacaa agttactgta
     3481 gttaaatcag caactttgtt tttatagtat aaatcaaata aatacattag tttaaaacaa
     3541 aatttttaat tattatgttt tgatatcttc catatacaga atattaatct aatttattac
     3601 aatgtcttca ttacgttctt ccatcattga ttgaatattt ttctgagcat ttaagagtaa
     3661 tttttttatc agttctattt tttcgtcatt agctctaaat aaaggcagtg ttacactaac
     3721 agcagctctt atacatccat tttttcttat tggtacggca agacatttta ggtgttccat
     3781 tgattcctca cactctgaag ctatttgtgt tctttgaact tcaagaagct ggtcgtataa
     3841 aacatcaaaa tctgtaattg atttttctgt aaaacctttt agaccatctg gatataagct
     3901 tattagtttt tccttgtcaa aatcacataa aagagctttt ccaagacctg tacaattagc
     3961 aggtattttt tttccaacat aagaaataag tctaatcggt tctggagaat ctacttttcc
     4021 aatataaaga acttcattat tgtcaagtat accaagctga caagtttcag aacaggaatt
     4081 aactatatta gtcatttcgt caattatata gttaaaaata gttctttttt caaaaaatga
     4141 agaccctaat gtgtaagaac gtatccctat agaatatttg gatgtgtttt tatcaatagt
     4201 aatgtatttt ctagcattaa gtgtatgaac tataggaaag atgctacttt ttggagcatt
     4261 gataacatct gctatttctg taagagtata tccattttcg gattctgcta atgcttctaa
     4321 aatatctaat atcctagctg ttgggcgatg attatctgaa gtcataaatt tccccctctt
     4381 tttattaatt taaagtatta gaatatgttg attttagcac actatacgat attttacaat
     4441 gtagaaaata aatatgtaaa tttatatata aataagctct tgatttagat acagatagat
     4501 atttttttag actgtacatt aaggaataac taatttccaa aatataccta aagaaagcaa
     4561 gcgatattta gttatataat ttaagtaaat atatgcaata gttattttta tatgtaaata
     4621 tcaaaatatt atgaacttat attttatcta ttttttacac tgtataaaaa ctttatagtt
     4681 ttgaatacta tataatttta aatacgctgt atttttgcat ataaggaagt catttgataa
     4741 tatacaaggt attatcagaa agtttgaaaa aattttgaca aaaatttatt ttttttattg
     4801 actcataaat aggaaaatgc tatcattaat tcaaagattc gtaattaaaa acttagttct
     4861 taaatacgaa ccgaaacggc cgttagaatt aaagctaaaa acttaataag agtaattaaa
     4921 tatcatgttt taggaggatt aactttatga taatggattt tattaaaaaa attcctgcag
     4981 gtatgatgat agtgccaatg tttatagcag ctttcattaa taccttttgc ccagatattg
     5041 tacaaatagg ttcatttacg acagcagtat tttcaagtgt tgggtctgca actataattg
     5101 gggttcaact ggtatgcttg ggaactcagc ttcaatttag agaaatgcca aaagttttaa
     5161 agagaggtgg tatacttctt ggttctaaat ttgctatagg tgctttgata ggtatactta
     5221 taggaaagct atttggaatg gatggtgtcc ttggtcttac aacattggct gttataagtg
     5281 cagttacaaa tagtaatggt agtatatatc tttcattgat gacttcatat ggagatgaaa
     5341 cagattgtgc aactatgagt ttacttactt taaatgatgg accatttttt acattaatag
     5401 ctcttgggac ttcaggtctt gcaaatgtac cattgttgtc attattggct gcaatagtgc
     5461 caatacttgt tggtatgata ataggaaatt ttgacaaaaa aatgaaagag tttttagagc
     5521 cagggtgtaa tttactcgta ccttttgtag ggtttgcact tggtagtagc ataaatcttg
     5581 caagtatact aaaaggaggt ttatcaggag tattacttgg attaatatct gtatttgtag
     5641 gaggtatatt tatagtattc tgtgataaat ttataggaaa acgtccagga tatgcaggat
     5701 gggctgtagc tacaactgct ggaaatgcaa ttgctgtacc tgctgctgtt gcattagttg
     5761 acccatcatg gcaaccatat gttgcaacag ctacaacaca agtggcagcc gcagtagttg
     5821 tcactgcaat attagtacct cttataacta gttggtgggc taagaagtat ggatgtccta
     5881 agttcccaaa acaaaatgag gatggtacaa taacagctta atttatatta cagtgagttg
     5941 ttggtaaaat aataaattct ttaattagat tttaggatat taataaaatt atattattta
     6001 taaaggagag caagaataat gataaatgca gtgataattg atgaaaaaga taatgttgca
     6061 gtagctattg aagcaatagc taaaggtagt gaaattagtt ttaaattaaa agacaaaaca
     6121 gtaaaaacga ttacagcact agatgatata actatttatc ataaattggc tataaaagat
     6181 atggccaaag gtgataaagt tactaaatat ggtgaacata taggagaagc aaatcaggaa
     6241 ataaaaagtg gtcaacatgt acatattcat aatgttagaa gtgttagaga ggatttggat
     6301 aaacaagtta tgactaatat ataaaataat atttatagag ttataaaagt tgtattcatt
     6361 gcttttagtg tcaaatagtt tttaaatagg tttttagctg tttgattagt gagtgtaata
     6421 aaaattataa tatataaaaa ttatagaaag tatatagact attttaggag gaaatcagta
     6481 tgaaatttta tggatataaa agaccagatg gaagagttgg tataagaaac cacatattaa
     6541 tattgcctac gagtgtgtgt gcatcagata caactagaat aatagcatca caagttcaag
     6601 gggcagttac ctttaataat cagaatggat gttcacaagt acattcagac caacaattaa
     6661 ctatggatgt tatggctggt tatgcagcaa atccaaatgt atatggtgta atagttgtat
     6721 ctttgggatg cgaaaattgc caaaatgatt tggtagttga tgcaataaaa gagagaacaa
     6781 ataagccaat aaaaactctt gtaatacaag aagaacatgg aactttaaaa actatagaaa
     6841 aagctgttcg ttatgctaga gaaatggcac aagaagcttc tttgcttaga aaagaagaat
     6901 ttccaatatc tgaacttata ctaggaactg aatgtggtgg gtctgaccca acaagtggat
     6961 tagcagcaaa tccattaata ggtgaattaa gtgataaact tgtcgattta ggagcaacat
     7021 caattttaag tgaaactact gaatttatag gtgcagagca tatactagct aggagagcag
     7081 taaatgaaga agtaaaagaa aaaatattac atatagtaca tagatatgaa aattcattaa
     7141 aattggttgg agaagaagtt agagaaggta atccatctcc tggaaatata gctggtggtt
     7201 taacaagttt agaagaaaaa tcacttggtt gtatacataa aggtggtcat agacaaataa
     7261 gtgaagtata tgattatgcc aaacaaattg ataagaaagg tcttgttata atggataccc
     7321 ctggaaatga tgcttcatct gttgctggta tggtagcagg aggagcacaa atagtggtat
     7381 ttagtacagg aagaggaaca ccatcaggaa atccaatagc accagtaata aaaataactg
     7441 gaaataaaat aacttttgca aatatggaag ataatatgga ttttgatgct agtccagtta
     7501 tatatggtcc acaaacaatg gaagaattaa cagatgattt gttaaatatg gttgtagatg
     7561 tagcaaatgg aaaacaatca aaagcagagt cccttggata tactgaaatg gcaatagcta
     7621 gagtatgcaa ctatgtataa tctttaagta agaaaataaa tacatagaat tttaataaag
     7681 ggtgagatta tgaagtttgt aagttttact gaaggagaaa atgaatgtaa aagaactgga
     7741 gttttttcta aagatgaatt atttattata gatgttaact ctttaaatct cagtagaaat
     7801 tttaaggatt taaatgaatt aattcaaaat gtatctagtg aagatattat taagttgaaa
     7861 actattataa atgaaagtac attctataat tatgatgttt ttaaaagaga gagatgtact
     7921 gtacatgttc ctatagaaaa accaatacat gatatcttat gtgtgggagt gaactataaa
     7981 gaccatcttg aagaaactca aactcacttt gatgatagtt ttgaagaacc acaaaaaacg
     8041 gtttatttta caaaacgtgt gacaaaggca ataggtccag aagatgagat agatggtcat
     8101 tttgagataa ataatcaact agactatgaa gttgaacttg gagtagtaat aggtaaaaca
     8161 ggtgttaata ttagaaaaga agaggtagaa gattatatct ttggttacac tgttataaat
     8221 gatatttctg ctagaaagtt gcaaaaagaa catgttcaat ggtttagagg aaaaggatta
     8281 gatacattta cctctttagg tccatgtatt ttacataaaa gtgcagttcc ttttcctata
     8341 aaacttaaag tatgtagtag ggtaaatggt gaagaaagac aaagttctaa tacaggatta
     8401 ttcatatcag acgtttcaga catagtttca gaactttcaa aaggtatgac cttagaggct
     8461 ggagacatta tagctactgg aacaccttct ggagtaggga tgggattttc acctcctcga
     8521 tatatgagta gtggagatgt agttgaatgt gagattgaga gtattggaat tttaaaaaat
     8581 tacattaagt aactgtttta atataattaa tattaaagct aaatctgatg tattaatgaa
     8641 catattaaaa taaattttag gagtttaaat tatgaaagca gaatttttga caccagttgt
     8701 tacaatattt gatgaagaag gaaatttaga taaagaaggt aataaaaaag tgtatgacca
     8761 tcttatagat ggaggtgtag atggactagt cataatggga agtactggag agttctttaa
     8821 tatgtctatg gatatgcaaa aagaacttat agatttagta actagtcatg taaataagag
     8881 agtaaaagtg tttataggta caagtcgtat gtgtataaat gaatctatag aactttcaaa
     8941 ctatgcacat aatagtggag cagatggtgt tatgataata agcccttatt atttctctct
     9001 tgatgatgat agtattgagt tattttatag tgaaatagct aagaatacag aggctgaaat
     9061 atatttgtac aattatccag atagaactgg ttatgattta agtccagaac ttacattaag
     9121 acttgcaaga aaacataaaa acatagttgg atataaggat acagtaactt tgatgggaca
     9181 taccagagat ttaataaata ctatgaaaat agagttccca gaatttaagg tttatagcgg
     9241 ttatgatgaa aactttatac ataatctaat gtctggggga gcaggttgta taggggggtt
     9301 atcaaattta attccagaag tttgctccaa atgggctgaa gcttacaata atagagatat
     9361 ggagaaggtt atagaaattc aaaaatatat taataaagca atggattttt acttaataac
     9421 caatccattt ataccagcaa tgaaaaaagc tatgaatatc agagggttac aaataagtga
     9481 ttatatgact gctccatttg taaaaccaaa tgaaaaacaa gtatgtgaaa ttaaaaaatt
     9541 gatgaatgaa ttaaatatat gttaaatcaa ccttgcaaag aggtcaacaa taaattacaa
     9601 ttatcttaat aaaaattaaa ttaaaataat actagggtac tatattagtt taatagggaa
     9661 agtgatgaaa attcactaca gcccccgcta ctgtatacgg atacgaaatc aaaatccact
     9721 gaaatttata aaaattttgg gaagggtgat ggaggaaaag ccgtgagtca ggagacctgc
     9781 ccagtattat ataagttcaa tttagggtgg agattgtaat ttataataaa atgaagtatc
     9841 aattcgtttt tgtaaagttg atatatgaac ttctccttat taaattagga gaagtttttt
     9901 attttattaa aaggttatac ttattaaaga tgactttagg ataactttaa taagtatact
     9961 ttaaaaagca tatattatta aagtttttta gccactcata aaaaataaga tattggagtg
    10021 aaagagatga aatttaaatc aaatgtttta aagcagtttt ttttaattgc catggtaatt
    10081 tttatggtag gaggtcttat agggtgtagt aaaagtgaca aagagaataa acaagctagt
    10141 actggtaaga aattagaatt aaaatatgct agtggattta gtgttgaaga tttagaagat
    10201 ggaattaaaa aagtaactga tggagataat cgagaactga ttttaatccc aaagaagatt
    10261 aaaattcctg aaaaatataa aaatgctaat atagttagaa cgcctgtgga caatgtatta
    10321 atagcatcta caacgcaggc ttgttctctg agaacaatag atgaactaga tagtataaaa
    10381 gccgtaacaa cagaagagca gtactggact attaaagaaa ttaaagattt aatgaaagat
    10441 ggaaaaatac attacgttgg ttcaaattta gcaccagatt atgaaaaaat agtagactta
    10501 aatcctgact taactatagt gagtagtggt ttatcagagg ctgatacaaa atttattgac
    10561 aaattaaaag aattagggct taattatgtt gtagacaatg agtataaaga gcaaaatcca
    10621 tttggaagaa tggagtggac aagtttaata gctgcatttt atgataaaga agatatggct
    10681 actagtgaac taaataaaac tgtacaaaag gtagaagaag taagtaaaaa aataaaaaac
    10741 aaagctaaac ctaaagttgt atgggcatct gtatatgaag gaagtgctta tatagctcct
    10801 aaagattcat atgtggataa tatgataaaa atggctggtg gaataaatgc atgtgcttca
    10861 atagattcaa atgaagggcg agtaagtatt gaacaattgc atgaagttgc aaaagatgca
    10921 gatatatttg tatactcatc aagttcagat tatgcaccaa atttagattc tataaaatct
    10981 agtgcacctg tattggctga tttggatgtt gtaaaaaacg gaaacctatg ggtgtttgct
    11041 ccagattatt atcaatcagt agataagacg gacgaattaa tagtagattt agttgagatg
    11101 ttccacccag gtactacagg tgaaaaaatc aaacattata taaattatga taaatagtac
    11161 agagaggaca caaaaagata ggttttatag attttagtta atatagcttt gctaagagga
    11221 gtatacaaat ggattttatc aataaaatga gaataaggaa tacaacaata tttatagtat
    11281 taattataag tatgatactt acttttttag tgtgtgtagc atttggctct gtaaaaattg
    11341 aatttaaaga agtaatagat gttgtattaa gaggtatgga taatacagaa cattataaaa
    11401 taatatacaa tataagaatc cctagagtac ttgcaaccat ggtaggtgga gcatgcctag
    11461 cagtatcagg tattttatta cagatatttt ttaaaaaccc aatagttgaa ccgtatatat
    11521 taggagtatc ttcaggttct actttatcag tagctctcat gattttggga ggatacacat
    11581 tgggatttga gagtgtaaat tcaatgagta tatttgtagc agcttttata ggagctttaa
    11641 ttgtaatgtc tatagtcatt ttatttgcaa ataaggtaaa gaatataaca actcttttaa
    11701 taataggaat tatgaccgga tatgtatgta gtggtataac aaatgtgtta caatcatttg
    11761 caaatagtga taaattaaaa gaatttacta tgtggtctat gggaagtttt tctggattta
    11821 catgggaaaa ggtagaaata cttataatag ttggtatatt aggtcttttg atgactgtat
    11881 tcataataaa acctttaaat gcttttttac taggagaaga gtatgcaaaa agtattggca
    11941 taaatataag attttttaga gttttgatag tatttatatc tagtatatta gcagcaatag
    12001 tatcagcatt tgcaggacct atagggttta ttggacttgc agtgccacaa atttcaaaaa
    12061 tgatatttaa aacatcagat aatagagttt tgataccagt agtaatattt ttaggcagta
    12121 tagttacagc tctttgtgat ttaatatcaa gagtaatttt atcaccagta gaattaccaa
    12181 taagtacgat aacatcttta ttaggagctc caattgtagt aatgttattg gtgaaaagga
    12241 aggatagggc ttagacatgt tgaaaactaa taatttaagt gtaggataca ataacaaagt
    12301 tgttataagt aatataaatg tagaagtaaa aaatggagaa atactatgtc ttttgggaag
    12361 taatggagct ggaaaaacta ccctattaag gtccttatct aaacttatat caccaataaa
    12421 aggtgaaata tatttgaatg gtgtaaacat aaattgtata tcaagaaaag cattatcaaa
    12481 aaaaatggct ttagtactta caaatagatt attaggtgat ttaatgactg tacaagatat
    12541 agtcaatata ggaaggtatc catatacagg attttttggg agtctatcta agaaagattt
    12601 aataatggta gatgaagcat tggagtctgt agatgcctta catttaaaga aaagatattt
    12661 tgatgaacta agtgatggag aaaaacaaaa ggtgttggtt gcaagggctc ttgttcaaga
    12721 accagaaata attatattag atgagcctac aactcacttg gatataaaac atagattgga
    12781 actcataaac atactgaaaa aattaagtaa agaaaagtca atatcagtaa tactttcatt
    12841 gcatgaaata gacatagcat taaaaagttg tgataaagtt gctcttataa aaaataataa
    12901 agtcatagct tatggtcagc ctgaagatgt agtagatgag gatattataa attcgcttta
    12961 tgaattggat gataaaaact ttaatagtct tttaggttca gtagaaatta gtaataaatc
    13021 taagaatgaa gtttttataa ttggtggtgg gggtaaagca acaccaattt atagagcttt
    13081 tactaaaaaa ggtataggtc tgtattctgg aataatacat gaaaatgata ttgattatga
    13141 aattggtaga actatgggga taaagatgtt tacagaaaat ccatttgagc ctataagtga
    13201 tgagagtttc gatttggcta ttagaaattt aaatgattcc aagattataa tagatacagg
    13261 atttagtgta ggagagacaa ataagaggaa tatagatatt ataaaagagg ctttaaaatt
    13321 ggataaaaaa gtttattctt ttagaaatag agatgagagt aagaagtatt atgacagttt
    13381 ggatagtaag atagaacata tagataaagt ttctcaaata atcaatagtg cagatataaa
    13441 taatttatta taatttatga aataaaatgt acttatgtgc tatagaaaaa agagtataga
    13501 ttataatttt tttgaaatta caatctatac tcttttgtta ttttgtttat gtatttatat
    13561 atttataaaa ctttagcatt ttatagataa ccaatagctt cctctctagt taaaaaaaag
    13621 tgaatccctg tagttgattc tttccatcta ttttcattaa aatcttcagc tacaatcgtt
    13681 tctcctactc tatatataaa attttcatca gcatatgcac gagcactttt aaaagattct
    13741 ttaccatcta tactagtaat tgacagtact tttgccttat cacatctaca agcatttgat
    13801 gtagcagaca ctctttttgc gtcgctgggt attaataatt gtaccattct aaaattaaaa
    13861 cattttttat agcctataaa tgctcctgtt tcaggacaat acaatttaaa aaaacgggta
    13921 ctttcatctg atattactcc atttaaagta gcatattcta aatttgaatt ttctaaatta
    13981 gctcctctaa tatctgagtt agataagtca caaaatctaa aacttgcacc acttaagtct
    14041 gcatttttta atatagaatt ttttaatgaa caatccgaaa gataagaatt attaaaaata
    14101 gagttttcta aatctgcttt ttctaaattt aaattgataa ggtcagacca cgaaaagtca
    14161 atattactta agcagtatcc tcttaagtct atattttcta atcttaattc ttttaaatct
    14221 gccttttctc cagcattatt gtttttaagc cataaataat gtttctcaag tgttttttct
    14281 aattcttgtt tagaaatagt tttcattaaa atcaccttca taaatttatt tttatatatg
    14341 tagtagcaaa atagtcatat atttaattat ataacaataa ataaaaaact tctaaataat
    14401 atataaaata tcatcttgaa agtacttttt tcaatatatc agaatatatt tttaagctat
    14461 ttagagataa tttaaatttt taaacaggac ataataagct tgtattgttt tatatttgag
    14521 ttgataaaaa tttctttaaa taaaaataat gtcaattata aagtaataaa agtttagata
    14581 taaaaaaatt tgtgtatata ctatatgtag tatataatag ataaagcact gactatatgt
    14641 agtgcttttg tttttgaaaa atacttattg catttctata attatagatt aattttggga
    14701 tttcaactga taagtaccaa aagaaataat aagcaaatta agtgaaatta tggcacaaga
    14761 ttatgttaaa aggacggtaa ataatgaatt ggatagagct taacaatcaa gtgataatta
    14821 aaaatgaaca aggcaagtac caattggaaa aggataagga agccttaagt agttacatag
    14881 aagaatttat acagccaaag ttgaaaaaat ttaataacct agaagaaaga ttgaagtatt
    14941 taatagatga aggttattat tcaaaagaag taataaatca gtatagcata gatgttatta
    15001 aaaatatata tgatatgatt gataaacata agtttgaatt tcaatcatat atgagtgcaa
    15061 ataagtttta tcaaaattat gcattaaaga gcaatgatgg aaaggaaata ttagaaacat
    15121 ataatgataa agtgttaatt gtagctttga cactaggaaa tggcaataaa gatttagctt
    15181 taaacttggc agataaaata gtaaaacaag aatttcaacc agctacacct acttttttaa
    15241 atgctggaag aaaaagggct ggagaaatgg tatcatgttt tttactatct gtagaagata
    15301 gtacagaagg tatatcgtat gctatatctt catcaaatca cttatcaaag attggtggag
    15361 gagttgcact aaatttatct aaacttagag catctggcga atctatcaaa gatatagaag
    15421 gtgcagctgg aggtgttgtt ggagtagcta aaatgttaga acaatcattt agttacttta
    15481 atcaaatggg agctagacaa ggttctggtg cggtgtacct tactgtattc catcctgatt
    15541 ttgaattgtt gatggatact aaaaagataa atgcagatga aaaaataagg cttgctacat
    15601 tatcattagg agctataata ccagataaat ttatggagtt ggcagaaaaa aatgaagtag
    15661 catatgcatt ttatcctcat actgtgtata agaaatatgg agtttctctt gatgagatag
    15721 aaatggatga gtggtatgat aaattggtta atgatagtga tataagaaaa aaagaaataa
    15781 atcctaggca gatgttgaca aaaatagcac agatgcaaca agaaagtgga tatccatatg
    15841 tagtgtacat agatacagct aatagagaac atacattaaa agatgttgga attattaaaa
    15901 tgtctaactt atgttgtgag atatttcaat atcaaacacc ttcagaaata gaggggtatg
    15961 gaggaaaaaa tgaatgggga caagatataa gttgtaacct aggttcttta aacatagcca
    16021 atgtaatgga taataaaact atagaaacta cagttgaaac agcaataaga gcactgtcat
    16081 ttgtagcaga tagcacagat ataaaaccag tgccaacggt aagcaatagt aacagcaagt
    16141 ctcattctat aggattagga gctatgaatt tacatggata cttagtaaga gaaaatattt
    16201 tatatacttc agatgatgct atagattttt ctaatgtatt ttttgcaatg gttagatact
    16261 attcaattaa agcttctatg aaaatagcta tagaaagaaa tcaaactttt gaaggatttg
    16321 ataaatcaga atatgttaag ggaagaaata gcaaggtttt atcaaaatac tatgaacaat
    16381 cttatctacc taagtcagaa aaggtaagag cattatttga agggatttat ataccaacaa
    16441 aagaagactg gacaaaatta ttggatgagg taaaagaaaa agggatatat aatgcatatt
    16501 taatggcagt agcaccaact caaagcatta gttatgttca aaatgctaca tcaagtataa
    16561 tgccaattac tgagccagta gaagtaagaa catatggaga ctctactaca atatatccaa
    16621 tgccattttt aacaaatgat aatatgttgt attatcaatc agcatacagg atggatatga
    16681 gaaaggtcat agatttagtg gcaacagttc aaaaccatgt tgaccaaggt atatcaacaa
    16741 cactgtttgt tacagatgaa aagacaacta gagatatagc tagacattac atatatgcct
    16801 ataaaaaagg tttgaaaagt ttatattaca caagaacaaa aatgacaaga gatactcatg
    16861 agtgtctagt gtgttcagta tagggagaga aatatatgac atttaattta cataaaatac
    16921 ataatgcagt taactggaat agagaagaag atggatttac acaagctttt tgggaacaaa
    16981 atgtaaaaca attttggctt cctgaagaaa tatctgtttc taaagacata aaggtatgga
    17041 atgaactaag taataaagaa aaggaattat ataaaaaagt attgggtggt ttaacattac
    17101 tggatacaaa acaggggaat aatggaatac catctatgat gagtttaaca gaaaacctac
    17161 aaagaaaagc tgtcttgtct tttatgggga ctatggaaga aatacatgca aaaagttatt
    17221 cttctatatt tatgactcta ctttctaatt tagaaataga tgaattattt gaatggatag
    17281 aaacagaacc aacattacaa agaaaggctg atttagtttt agctcaatac gaaaatacta
    17341 caaatcaaga gggattatac ctatcaatgg ttacaagtgt atttttagag agtttcttgt
    17401 tttattcagg attcttttat ccattgtatc tatctggtca aggaaagatg gtagctagtg
    17461 gagaaataat atcgcttatt cttagagatg agtctttaca tggtaagtat ataggattgc
    17521 tagcacaaga aatatacgat tcatttgata aaatggataa ggagatgtta gaagagaaga
    17581 tgtattctat actttatagc cttatggaga atgagataga gtatacaaat gttatataca
    17641 gagaaagtgg gcttgaaaaa gaggttgtga actttttaaa atacaatgcg aatagagctt
    17701 tagaaaatct agggtttgag aagctatata ctgtagatgc tataaatcca attgtattaa
    17761 atggtcttag cactgaaacc aaaacacatg atttcttttc aactaagggt aatggatacc
    17821 aaaaaggagt gtatgaggaa ttagaggatg aagattttat aatataattt ataattatga
    17881 cacactgttg tcacttttaa cgtgataata tcaatgtata aaatatatat ggaggtattt
    17941 ttatgaaata tgaatggaga aaaagagata aagagttata tttacctaaa aagaatcctg
    18001 taatagttga aataccagaa cagaaattta tcatgttaaa agggagtgga gaaccaaatt
    18061 ctaaagaatt tactgaagca ataggagtac tttattcttt ggcatatgca ataaaaacaa
    18121 tgcctaaaaa aggaattgta ccagaagggt attttgacta tacggttttt cctttagaag
    18181 gtatatggga taagggagaa aaaagtaaag gcttagatgt acttattaaa gaagatttaa
    18241 tttacactat aatgatacgt cagccagatt ttgttacaca agacctgttg gataaagcta
    18301 ctgatatagt aaagaagaaa aaacctcata atcttcttga aaaagttaga tttgggtcta
    18361 ttaatgaagg gctatgtgtt caaatgcttc atataggttc atatgataat gaaccagaaa
    18421 gctttgacat tatgaacgaa ttttgcaaaa aaaataaact tataagaaaa tcttacacac
    18481 atcgtgaaat ttatataagt gatgctagaa aagtatcgcc tgaatcattg aagacggttt
    18541 tacgttttga agtagaaagg gaagtataaa tattttgaat aatttataag agttataaaa
    18601 agtgaagtct gttgagactt cattttttga tgcaatttta aaattaaatt tgatgtttca
    18661 taatatttta aaaactttct acttttatta gtgtgaataa ctgttgctat ta
//
LOCUS       DAEORZ010000077        18620 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEORZ010000077 DAEORZ010000000
VERSION     DAEORZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.077"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            198..1415
                     /locus_tag="KRH39_002325"
     CDS             198..1415
                     /locus_tag="KRH39_002325"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBG8512224.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1510..1890
                     /locus_tag="KRH39_002326"
     CDS             1510..1890
                     /locus_tag="KRH39_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG8512225.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2174..2656
                     /locus_tag="KRH39_002327"
     CDS             2174..2656
                     /locus_tag="KRH39_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8512226.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVNLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIMVVLNHSEFSEINCMVENEVVAEKDTVIEIMK
                     "
     gene            2677..3492
                     /locus_tag="KRH39_002328"
     CDS             2677..3492
                     /locus_tag="KRH39_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG8512227.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIRLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3545..5095
                     /locus_tag="KRH39_002329"
     CDS             3545..5095
                     /locus_tag="KRH39_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG8512228.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFLKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGKLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGIEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5192..6544
                     /locus_tag="KRH39_002330"
     CDS             5192..6544
                     /locus_tag="KRH39_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG8512229.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKKRQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAESLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSCSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6675..7433
                     /locus_tag="KRH39_002331"
     CDS             6675..7433
                     /locus_tag="KRH39_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8512230.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDNN
                     GLEKLRQQGTFLISNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7480..8199
                     /locus_tag="KRH39_002332"
     CDS             7480..8199
                     /locus_tag="KRH39_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG8512231.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8323..9723)
                     /locus_tag="KRH39_002333"
     CDS             complement(8323..9723)
                     /locus_tag="KRH39_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBG8512232.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9727..10635)
                     /locus_tag="KRH39_002334"
     CDS             complement(9727..10635)
                     /locus_tag="KRH39_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8512233.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10896..11543
                     /locus_tag="KRH39_002335"
     CDS             10896..11543
                     /locus_tag="KRH39_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG8512234.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11868..14507
                     /locus_tag="KRH39_002336"
     CDS             11868..14507
                     /locus_tag="KRH39_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG8512235.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14565..15668)
                     /locus_tag="KRH39_002337"
     CDS             complement(14565..15668)
                     /locus_tag="KRH39_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBG8512236.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16028..16849
                     /locus_tag="KRH39_002338"
     CDS             16028..16849
                     /locus_tag="KRH39_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG8512237.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16928..17139
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17226..18542
                     /gene="argH"
                     /locus_tag="KRH39_002339"
     CDS             17226..18542
                     /gene="argH"
                     /locus_tag="KRH39_002339"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBG8512238.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         7112 a         2143 c         3295 g         6070 t
ORIGIN      
        1 atttttaaat tatcatttaa tgtaatttaa atgaattgaa gatatatgtt tattgtatgc
       61 aattgttgta tttttaggtt ttttgtgatt aattaaaaaa ttataaaaat cgcttacatg
      121 ttgattttta aaaaaaaaag atatagaatt atatagaaag taataaacaa aaattggatt
      181 tacgggagtg gaatgaaatg atgaatgtta ccttaacaga tgtaaaagag gctagagaga
      241 ccattaagaa catagtaaaa agaactgact tacttgaaag tgttaaactt agcgaaaaaa
      301 caggagcaaa tgtgttctat aaatgtgaga atcttcaaaa aacaggttct tttaaattaa
      361 gaggagcttg taacaaaata gcgagtttga cagatgaaga aaaagcaaat ggagttatag
      421 cttcaagtgc tgggaatcat gctcaaggag ttgcattggg ggcaaaaatg actggtataa
      481 aatctacaat agtaatgcca gcaactgcac ctttagcaaa agttagtgca actaaaggtt
      541 atggagcaga agtagtttta aatggtgcgg tttatgatga tgcatatgca aaagctgttg
      601 aaatacaaaa agaaacagga gcaactttct tacatccatt caatgataag tatgttatag
      661 caggacaagg aactatatct cttgaaattt ttgaacaatt agacaataaa gttgatacta
      721 tactatgccc agttggtgga ggaggaatca tctctggtgt tgctgttgca gcaaaagcat
      781 taaatcctaa tgtaaaaata gttggtgttc aaactgcaaa tataccttca atgaaagagt
      841 caataaaaaa tggaaaagta acaacagcat ttaatgatac tacaatagct gatggtatag
      901 ctgttaaaac tccaggagat ttaacttttg aaataataaa tgaattagtt gatgaaatag
      961 tagtggttga agagacagaa attgcagaat caatattatt tatgatggaa agtcaaaaaa
     1021 tagtttcaga aggtgctggt gctgtatgta cagctgctat attaagtggt aagtatgtac
     1081 cagcaaaaga tgaaaatgta gtttgtataa tatctggtgg taatatagac ataaatacat
     1141 tatatagaat aataggtgtt gctttagcta aagaaggaag aagatattca tttagtacaa
     1201 ttatggaaga taaaccagga aactttgctg aattaactag gataataagt gagaatggtg
     1261 gaaacatttt aagtgctaat caaggtaaat tatcagcagg ggaagctcta ggaaaacaaa
     1321 gtgcggaatt tatattagag acaatagatt atgaccatat agctagaata aagaaagcta
     1381 tagaagaaaa aggttttaaa attatagaat tataataaaa agtgtatata atatacaaga
     1441 aaaatagtat aatagtgtat gaagatacat tttatgtgta ttagattaac gaattaggag
     1501 gattttaaaa tgaaacatga agtaatacat actaatgatg caccagcagc tttaggacca
     1561 tattctcaag ctataaaagc tggaaactta ttatttgtat ctggacaagt tcctcttgta
     1621 ccagagacta tggaagtagt tgaaggagat gtacaagctc aaactgctca atctttaaaa
     1681 aatcttaaag ctatattagc tgaaagtgga gctgattttt caaatgtagt taaaactaca
     1741 gtatttataa aagatatgaa tgaatttggg gctataaacg aagtttatgc tgaatatttt
     1801 ggagaaaata aaccagcaag agcttgtgta gaagttgcta gattaccaaa agacgttaaa
     1861 gttgaaatag aagtaatagc tgtattataa aaatacaata tttttaattt gaaaaggctg
     1921 aaaaactata ttctagtgat tcagcctttt tataaaaaaa taaatcacaa taaagagcat
     1981 gataaatatt gtcgaattaa aaaataaaat taaaataaat attgcaatat ttttgagaag
     2041 gttttataat gagtatataa tttgaaacgt gtaactatta aattaggcat gagttcttgg
     2101 atatttttat cctgaggatt catgcctttt ttgtattgaa agtcaaaaat ttatttaata
     2161 aggaggaagt actgtgttaa attttttcaa gaaaaataaa agctataagt tacatgcagt
     2221 agtgagtggt aacagcatta atattgagaa agtaaacgat tctgtgtttt caaaaaagtt
     2281 aatgggagat ggagttgcaa ttattcctaa tagtaatgtt gttgtagctc cttgtaatgg
     2341 taaagtaact gtgcttacag agtcaaagca tgcatttggt atggtctcag atgagggtgt
     2401 tgaaatatta gtccacatag gaattgatac agttaattta cagggtgagg ggtttaaaaa
     2461 tgaagtgtct caaggagata ctgttaaaaa aggtagtcca ataataagct ttgaaagaga
     2521 aaaaataaat tctcagggaa ttgattgcac aacaataatg gttgtattaa atcatagcga
     2581 attttcagaa ataaattgca tggtagaaaa tgaagttgtt gcagagaaag atactgttat
     2641 tgaaattatg aaataatagg aagggattaa ttcaatatga gagtagtaaa aatctttaat
     2701 aataatgcat tatctacagt aactaatgat aggaaagaag ctattttact aggtcttggt
     2761 ataggtttta ataaacgtcc aggagataaa gttaatgaag ataaaataga aaagatttat
     2821 tatgttcaag accacatgca aacaaaattt ttagaattac taaaaaatgt tagtccagaa
     2881 gtgatggatg cttcacaaca aattataagt cttataccag gaaatgaggg gaaatttaat
     2941 aataaaggga tattatcttt agtggaacat attagttttg caatagatag aatgaaaaat
     3001 aatgtttttt tacctaattt aatgcttttg gatataaaga tgatgtattc aaaagagttt
     3061 gaattaggag taaaagcttt agaaattata tatagagtat gtcatattcg tttgcctgaa
     3121 gatgaagcag gttacatagc tttacatttt gtcaatttac aatcaaatga taatttggca
     3181 tatgatactt taaaatttgt aaaaggtagt attgatttga ttaaagaatg ttatggatta
     3241 gaattagacg aatcgagttt gagtacatta cgttttagaa ctcatcttaa atttcttgct
     3301 caaagaattt ttaagaatga aatttgtcag gatgataaaa tgattgaaat gtatgattat
     3361 ttaattaaca atcatcctaa aaataaagaa tatttagaaa agttaaatgt atatattgaa
     3421 aaggagttta gatataaatt agataaacct gaaaaaattt atctactact acacctaaca
     3481 aaaatcttat aagaatgaga ttaattagta atagaaatat gtataatttt aaggggggat
     3541 aagattgaaa gagaaactac ttggaggact ggaaacattt gcaaaggcaa tggttcagcc
     3601 attaatgtat ttatcaagtg cagggatatt aatggtggta ggtgtactac ttacaaatga
     3661 tatgttatta ggagtacttc catttttaaa atgggaacca attcaaatat ttggaagctt
     3721 aatttataac tgtataatgg ctattattaa taatcttgct gttatatttg ctataggtat
     3781 tcctgctgca ctagcaaaaa aagagaagca caaagcagcg ttaataggat ttatgagtta
     3841 tataatatac cttacagctt ctaacactat gttaacatca ctaggtaagc tagcaaaaga
     3901 aagcaagatg cttggactga tagggactgg acaagctact attttaggta ttcaagttgt
     3961 agatacaagt gtatttggtg gaattatttt agggtgtata gctggatatg tatttaatcg
     4021 tacaagcaac aaaacattta aaggagcttt acagatatac tcgggagcaa attttagttt
     4081 tatatgcatg ttctttgtat ccattttaat tggagttgtg agtaacttta tatggccatt
     4141 agctcaacaa ggaatagctt ctcttgcaaa tgtcatcaaa gatacaggta catttggttt
     4201 attcttatat ggttttttag agagaatatt agtaccaact ggacttcatc atttaatcta
     4261 tacaccattt cagttttctg atttaggagg agtgcttaaa gttggtgcag atacagtttc
     4321 aggagcctat gctatagtta tgacagaaat gaatttacca gttgagcgtt tctcagatag
     4381 tatatactac atggcaactg gatttacaaa gatgtttggt tatataggta taggagctgc
     4441 ctttatttat actgcatata agcaaaataa gaagaaggtt aaggcaacga ttattccact
     4501 aataattacg gcagtattag cctctgtaac agaaccatta gactttatgt ttgtattctg
     4561 tgcaccactt ttattctttg tacatgcagt tatagctggg ctttttatag ctttattaaa
     4621 aatatttgat gttactgcac tatgtggagg taacttatta gtatcaatta taatgaatac
     4681 tacagtaggt atagaaaaaa cacactggcc agtaatgatg atattaggac ttgcacaaat
     4741 tattgtatat tttgtagtat ttagtttttt aattaagaaa tttaattata aaactccagg
     4801 tcgtgaagat gagagtacag ctacaggcga agaacaatca aaacaactta atttagatgc
     4861 aggtatagaa aatataattg atgggttagg cggaaaagaa aatattaata cagtagaaaa
     4921 ttgcatcact agattacgtg ttaatgtaaa agatgaaagt aagattaatg aagatataat
     4981 taatttaacg ccaaatagcg ggattgtaag gaaaggaaaa gatatacaaa taatttttgg
     5041 acttcatgtg catgaggtaa gacgtgcagt agaagatttt ttagaagctt attaaagata
     5101 tatatttata tagcaagggt aaataacagc tttaagtgct gtttactatt tattaaagat
     5161 acatataatt tatagaggag gaataattat tatgattatt actattgttg gtggaggaag
     5221 cacatttact ccaggtattg ttaagtcaat tgcattaaga gaaaaagagt tagagcttga
     5281 ggaaattcgt ttatttgata ttgataaaaa acgtcaagat aaagtagctg tagttgtcaa
     5341 ctggatacta aaagaagatg ttaaaacaga tgttaaacta actgtaacta attgtgaaaa
     5401 agaagcgtat acagatgcta catttatatt tgcacaaatg cgtgttggta aatatgaaat
     5461 gcgtgagcag gatgaaaaaa ttccattgaa atatggttgt gtaggtcaag aaacttgtgg
     5521 ttgtggaggg atggcatacg gactgcgtac aatacaacca atgataaagg ttattgatga
     5581 tgtagagaaa tatgctaaaa aagattattg gatattaaat tactctaatc ctgctgcaat
     5641 cgtttctgaa gcttgtcgta agttaagacc aaatgcaagg attataaata tctgtgatat
     5701 gccaatagct attatagata tgatagcgga gtcattaaat atcaatgacg ttcataatat
     5761 acgctatgat tattttggtc taaatcattt tggctggttt acttcaatag attataaaca
     5821 tagagactta atggaagaga ttaaggaata cataaaagaa aataaaatat tattaccaaa
     5881 atcttatata gaaaacttaa ataaaagttg tagtacagga aatcgccatg ctgtaagtag
     5941 ctggatacat gtttgggaat ctgtttatac aatgttagaa agcttcccaa aatatctgcc
     6001 aaacacttat ttaacatact atcttttaag taaagaaaca gtggaacatt caaacataaa
     6061 tcgtactcgt gcaaatgaag ttatggaaag ccgtgaaaaa gatttatttg aaggtgtgag
     6121 acattactta gaaactggag aaatttctga aaaagcattt tatgcaggtt cacatggaga
     6181 ttggattagt gacttggcag tttcaattaa aaatgataca cgtagtcgat ttttagttat
     6241 aactgaaaat cgtggagcta taccaaacat gccatatgat gcaatggtag aaattccagc
     6301 atatattggt aaagatggtc cagaagttat tgctcgaggt ccaatacctc tattccaaca
     6361 aggattaatg atgcaacaac ttaacagtga aaaattgata gtagaagctt atatagaaaa
     6421 tagttatgaa aaagctttac aagcattcac attaaataaa actattccaa gtatgaatgt
     6481 tgctaaagaa attttggatg aaatgataga agttaataag gaatactggc cagaattaag
     6541 atagtaggat aaaagaaagg gtttttattt aataattaga tatgttttca gagtctttta
     6601 tcaaaatgta ttcacaaaca tatggcaata tattaaaata aagagtgata tttttagaag
     6661 aatggggtga tgcaatgaac attataaata aaattttttg gcaagaagat aggattggca
     6721 tgattacaaa taatttagag gcagatacac atagtcactg tatgttacaa ctgttcttag
     6781 ggatagaaga cagcattgaa ataacagtta atgaaaaatt agttaaatgt aattgtatca
     6841 tagtagataa aaatatctct cattcatttt cagcaagaaa aaaagtttat tactctgcta
     6901 ttattgaacc tacatctata tatgcagaac agcttacctc aaaaatgaat gattttggct
     6961 actggatatg tgacaataat ggattagaga aattacgtca acaaggaact tttttgatat
     7021 ctaactctag taaagaacaa tatttaagat ttatggaaat gcttaataac tatttgaata
     7081 tacctataac tctaaaacat tatgatgaca gaattactga attgctcaat ttattacgta
     7141 cttgtaattg tgataatcat actatttcaa gttttgcaga taaagtgtct ctatcagcaa
     7201 gtagattatc acatctgttt aaagaacaaa tagggatacc tttaaaaagc tatatcttat
     7261 ttcatcaaat ggagtgtgct tttagagaat tactttctgg gaaaaatgtt accgaagctt
     7321 ctatgactgc tggattcgat actccatcac actttgcagg gacagttaag cgaatgatgg
     7381 gaatgcctgt ttctttatct ttgaaagata gcgaattttt gaaagtatat taaatattct
     7441 tgttatataa tgaagttgta ctaagaaagg ggctttgtta tgaataaaga atatttagag
     7501 gaaacaaaaa tgttaaatta taatgaacca caacttaagc ttttggtatc atcaaagaat
     7561 tggttggaat tagatgattt tcataaaata aaatctattt atgagtttgt acagaatgat
     7621 attttatttg gatataatgc ttctgatatg ttaagcgcaa cacaggtatt aaatgatggt
     7681 atgggacaat gcaatacaaa agctactctt ttaatggcat tattacgtgc agtgaatatc
     7741 ccttgtaggc ttcatgcttt tgatgtaaca aaagattttc aacgaggagc tacatccaaa
     7801 ttgatttcac tattagcacc aaaatatatt cttcatactt gggtagaggt attttatcaa
     7861 gataggtgga ttgctctgga gggtgtcata actgataaaa aatatttaga agcaattcag
     7921 aagaaatttt ttaatcatgg tggaacgttt aaaaagtatg caatagctac aaatgatttg
     7981 aaaaatacat ctattgattg ggatggtaaa gatactttta tccaaaaaga agctatcgtg
     8041 tatgattatg gtatttttcc atcaccagac gtattttttt ctactcattc acaacatatg
     8101 tcaaaattaa aaaatttcat atatgtacat cttatcagaa aaataatgac aaaaaatgta
     8161 tgtaaggcta gaaacaatta tattgataaa aacgaatgag tgtttaagag gtaactatat
     8221 aggaaatata tagaataatt aaaactgtac tggttaaaat gaataaaaag gagttatcac
     8281 agagcaaact taaattgttc ataggataac tccttttctg tattaacttc taataatttg
     8341 atgtcttttt ataaaacata tagatacaat taaaccagta aatgcaagag tagctgctat
     8401 tgaaaatgca acatctattc catgaatgtt agacagcatg tttgaactca tatgagcttt
     8461 cttagtagca cttgtcatta tagtaataag tatagcagaa cctatagctc cagatatctg
     8521 acggagtgtg ttattaattg ctgtggcatg agatataagt tctctatcta atgttttaat
     8581 tccccatgta gttaatggca ttaaaaccat tgaaatacca atcatacgaa tacaatacat
     8641 caatgacaca tgaattaaag atgtatctct gcctaaaaaa gcgaaggata aagttcctaa
     8701 aaacaaacat cctgttccta atattgatag agcatgaggt ccatatttat ctaaatgacg
     8761 tcctgcaatt ggattgagta caaccatcaa tatagcacca ggtaacatta atgaacctga
     8821 agataatgca gaaaatcctc ttacagattg aacgtacaga ggaatcatta atgtagcaga
     8881 catcattgag gcatatacaa tcataattaa tattgtagaa ataagaaatg gtttattttt
     8941 aaaaaccctc aattctaaaa aaggttcttt agattttaat tgacgcaatg taaataaaat
     9001 taatgacata attcctatta atattggtaa atatgtggca atattagtcc acccataatt
     9061 accttgatta gtaaatccaa ttaataaacc accaaaacct agagatgata gtactattga
     9121 aggtatatct aattttagtt tttgagtttc cccaacattt cttaatagaa taaatgatag
     9181 tataacatct aatatagcaa taggggaaag aaacagaaat aaactatgcc aaccaaatga
     9241 atctactaac catcctgata aagttggacc tattgctgga gcaaatccta ctgtaatacc
     9301 aactaaactc atagctgcac cacgtttttc ctcagggcat aaatagagta taataacttg
     9361 taaaagtggc attagagaac cagaacccat tgcctgcaac acacgagcaa tcaacatagt
     9421 tgaaaaatta ttagaaaaaa gtgcaataat acatcctata gaaaagatag acattgatgt
     9481 aataaataac tttctagtac taaatcgatt tattaaataa gctgttgtgg gtacaataat
     9541 acctgcaata agtagataaa ttgttgttaa ccactgacca acatcagcag taattttaaa
     9601 atctgacatt atattaggta atgctgttga caatagtgtt tgactgagag aacttataaa
     9661 agcaccagtc attacaattg ctattattaa atttcctttt ttattgttac tttgggtttc
     9721 cataatttat aacgtctcct tttttaattg atttttaatg ctgttcaata aaagtacttt
     9781 tggtcttaaa gtgtcttctt tttctaaata tgcaatcatt gcatccataa ttaaagaatc
     9841 atattgttga ttttttccct cgacgtataa tttttttatt gcttctagta atttaggatt
     9901 ttcaaggaga gaagtatcag ttttgattag ttcttctata tcttgtatcg ttttaaaaaa
     9961 tagcgattta ttatgaaggg aaaagtaaac ttcatcttct tgtttatgta aaaacattga
    10021 tttttcaatt aaaggattta gattatccat attttctaca actaaatcta gaatttgtat
    10081 agtaaatttg gcaataaagt atacatttat ttctcttttt tgtacatgaa taatgtatat
    10141 aaactgatga attagagcat ccaataagaa aactaaatca aatataattt tttctatttt
    10201 atcaccatag ttatcaaata agcttagttt gtattcattc aataagttag ctcgtgattt
    10261 tgtttgcaat ttacttgtag ccttacgttg ctcttcagaa aagttttctc ttatataggt
    10321 atttatggta gttctagaaa aaatattttt ccatacagca attatctttc tgtttaactt
    10381 ttctgatgat ggaatgtcat tattttcgtt tatggaatgt ataatttcta aaaacttttc
    10441 atcataataa ttgattattt cgcagataaa actttcctta gtttcaaaat gcttgtaaaa
    10501 agtagcttta gacattttac actcttttac aacatccgat attgaagtat tttgaaaacc
    10561 cttttggtca aaaatttttt gtgcaatagt tataatgagc ctttgttttt ctgtcacttt
    10621 attatctcct ttcacgcaaa aacgtttagt actatatagt acctacaagt tttaataaat
    10681 atattataca tcatttggaa aaaaaatcaa tatatagact gtaatatgaa atttttgaaa
    10741 aatttgttgt tatttttagt cgtataaaaa agaaacttaa gagtttctta agaattgttt
    10801 aaagaagttg caataaatta gtggtatgat tatagtatga aagagaatgt cttgaaattt
    10861 ttctgtatca aattccaatt atggaggtgt agtatatgaa aaagaaaatt tgtgtagcaa
    10921 taatttttgc tctatttata gtttttgtag aaatgtttat tgatagctct agcgaaggaa
    10981 gtaaaattat gacagctgaa ttaagtaaat acaggaaagg cgtaaatgta ttatcaataa
    11041 acaatattat agtagcgaat aaaaaatata gtttgcccaa agattattct cctcaggaaa
    11101 gcagtgaagc gagagatgct ttttataaaa tgaataaaga tgctcaaaag tcaggattaa
    11161 atttaaaagc ttttagtaca tatagaagtt atgaatatca agatagatta tttaaatcat
    11221 atgtaaaaga acatggagaa aaagaagcaa atagattttc agctaaacct ggagagagtg
    11281 agcatcaaac tggacttgct tttgatattg gaggagatga ccagtcttgt tgggcaaata
    11341 aaaagttcaa caatacaaaa gaagctaagt ggttgtatga aaatgcatat aaatatggtt
    11401 ttatacttag atatccagaa ggtaaagaga atataacagg atatatgtat gaatcttggc
    11461 actatagata tgttggaact gaacatagca aaaactttgc aatgaataat ttgactttag
    11521 aagaatattt acatataaat taagagatgg tttgttaaaa ttaggttaaa ataaaaaata
    11581 acctagacta ttagctgtgt aaagtgatat aatgtaaata caatttaaga taactactcg
    11641 aaggggagta gcttacacga aagtggtaat taatcgtcaa gacagtagaa atactcggtt
    11701 aattagcaag ggattccttg atagcaagac cttcaaaata agcattaggt atgtttgttt
    11761 tggggtcttt ttttattcaa ggaattttta tttaaaaaaa tttaaatttc aagtatacat
    11821 aagagccccc aaacataaaa taaatttatt gaagaggagg gcaacaaatg tcttacaaaa
    11881 gtacttatca agaagtaatt aaaaatttag actcaagttt atctggtttg agtaaagaaa
    11941 gagcagaaga gttgttagaa aaaaatggag ctaatgaatt aaaagaagca gataaggttc
    12001 ctacttataa gcttttccta gaaagtttta aagacccatt agtaataatt ttattgatag
    12061 ctgctttagt acaaatattt ttaggtgaaa cagtggagtc tataataatt tttgcagtta
    12121 ttattataaa ctctgttctg ggtgttgtac aaacaaaaaa agcagaaagt tcattagaaa
    12181 gtttgaaaaa tttatctgca ccaaacgcaa aggtaataag agacaataaa aaaatgacaa
    12241 ttccagcaaa aaatctagta gttggagata ttgtattctt agaagctggc gattacatac
    12301 cagcagatgg aaggcttata gaagcacaat ctctcaaggt tgtagaaggg atgttaactg
    12361 gagaatcaga accagtttta aaacatacag aaaaaataag tgaagatgta agtttaggtg
    12421 accagaaaaa tatggtattt agtggtgctg tagttgttta tggtagaggt tcttttgtag
    12481 tgactggaac aggtatggat actgaaatgg gtaaaatagc tgggctttta gaaagtgctg
    12541 aaaataaact tacaccattg caacaaaaaa tagaggattt tagtaaaaaa cttggtgtag
    12601 gtattgtaat acttgctctt ttaatattta taattcaagt agcaagaaat tactttatgg
    12661 ctggaaatgg agaaatgaca caaattattt tagattcatt catgttctct gtagcagtag
    12721 cagtagcagc tataccagaa gctctatcat caatagtcac tatagtattg gctgttggaa
    12781 caaatagtat ggcaaaaaaa caagccataa taagaaaatt accagcagta gaaactttag
    12841 gttcaacaag tgtaatatgt actgataaaa caggtactct tacacaaaat aaaatgacag
    12901 tagttgatta ttatatgtat ggaactaata atgatgagtt agataaagaa aaagtaaact
    12961 attcttatca tgctaaatta ttgactatgg tttctacatt gtgtaatgac tctcatataa
    13021 ctagtgatgg taaagaagtt ggtgacccaa cagaagtagc tttaataaat tattcaagta
    13081 aaaatgattt agattatgat aaattaagaa gtaagtatat cagaataaat gaaataccat
    13141 ttgactcaga cagaaagctt atgtctactg taaatagtat atatggagat tatataatgt
    13201 ttacaaaagg tgctccagat attgtgttta gtagatgtaa atatgctttg aaaaatggtt
    13261 caaaagtaga tataacagat gagataatag aagaatataa acataaaaat gaagagtttt
    13321 caaatagagc acttagagta ttagcttttg caataaaaga tgtcccagaa gaagatttta
    13381 taccttcaat tgatgacgaa catgacatga cattggttgg aataatggca atgatagacc
    13441 caccaagaga agaagtaatg gatgcagtaa aagaggcaaa gagtgctggt ataaaaactg
    13501 ttatgataac tggtgaccat aaaacaacag cagcagcaat tgcaaaagag attggtataa
    13561 tggaagaagg agacttggct cttactggaa aagagcttga tgctttgagt gaagaacaac
    13621 tatatgaaaa actagaaaat ataagtgtat atgccagagt ctcgccagaa aataaaattc
    13681 gtatagtaaa agcatggcaa cataagaata atataacagc aatgactggt gatggtgtta
    13741 atgatgcacc tgcattaaaa caagcagata taggaatcgg tatgggaagt ggtactgatg
    13801 tagcaaaaga tgcttctgct atggtattgg ttgatgataa ctttgcaagt atagtaaatg
    13861 ctgttgaagt tggtagaaca gtatacagta atataataaa atcaattact tatctatttg
    13921 caggaaactt aggagctata gtatccatag tatttgcagt gtttgcaggt tggtcaaatc
    13981 catttacagc tttacaactt ttgtttataa acttagtaaa tgactcttta ccagcaattg
    14041 cattagggtt tgaaaagtca gaaaaaaata taatgaataa acctcctaga cacccggatg
    14101 aaagtatact agcaggagga acattgaagg cagttgctat aagaggttgt ataataggtg
    14161 tagttacaat aattgcacaa tatataggat tacaagtatc tccagaactt ggagtagcaa
    14221 tggcattttc aacattgata ttagctagaa taatgcaaac attacctgca cgttctaata
    14281 atcaaacagt aataaaatta ggttttttca gcaataaata cgttatagga gcagttttag
    14341 tctgtcttgg gctttactct ttaacactaa taccagtgct aagaggaata ttctctatac
    14401 caacaacgtt tggattgtac cagttgggga tttgcctagg gttagcatta atttcatcaa
    14461 tattaatgga acttaacaaa ttaataaata gaagtgaagt aaaataatca aaaaattaaa
    14521 gggcatattg attttaacaa tatgcctttt ataaatagtc taatttattc tatattatcg
    14581 attatttgga atgcttttat aacttcaggt aattcttttt cttcaggcat tctaaatctt
    14641 ataaaatttt tatcaagtcc agtaaaatca cttccagata taactcttat ttttaattta
    14701 gcgaactctt gttgtaaatc tatgtccttg tttttatgtt caactgtcat aattgataca
    14761 gtatcactag tttcagctat attaaggttt ttccatggaa ttaataattg attttttata
    14821 tcttttgttt tttcttttaa ttcttctata aattgaacat catctaataa atttgctgca
    14881 atactacgag atatttcaga cacttcgtaa ggagtagaaa tctttttaat agggctaaca
    14941 agttgttctg gaagaactgc atatcctgct cttagtcctg ctaaaccaaa tccttttgaa
    15001 aatgttttta aagcaattac attatcataa ttatttagaa gcttgacagc tgaattttct
    15061 tttggcatgt attcaccata agcttcatct accataactg ctatatcata tttagctgct
    15121 tcctttacta tattttcaat tgatgataaa ggtataattt gtcctgtagg attgtttgga
    15181 ttgtctatgt aaattacttt atattctgga tttatttttt caataaattc tttttcatta
    15241 aacttgaaat tatcctcttt ctttaaaaga acataatcat aagttgcacc atgcattttt
    15301 atatctgtct catattctga gaactgaggt gaatatccta aaactttatc tcccttttca
    15361 ataaatagtc tatttaaaag gtaaataaca tgtatagaac catctgccaa acaaattctg
    15421 tcagtatcta atgctataaa atctttccaa taatcgacta tattatcttt taaagcaatt
    15481 gaatgaggat atcctcttat catatcaaat tttaaagcat taaaagcttc tacagctttg
    15541 tttgagtaag acacggtatt tataccttca ccacagtcta tctcgaatac atcttctgaa
    15601 tgtccagatt ctattgcata actttttttc aactgcattt caatagcttt gttaattttt
    15661 agtttcatac tttatcactc cttatttagt caataataaa tgttataaaa gttgctagat
    15721 ttaaaatatt atgaaaatat ataatagtaa aaatgaatat aaatattata atagctcact
    15781 ttatttaata tgattatata tcatatattg taaaatttct agcattataa gactataaat
    15841 tgaaaatatt cagaaaaata ctacaagaaa cgatttattt tgctcttaaa ataagttaaa
    15901 tgaatgttat taattttgta aattgaaata gtttaagtat aaagttatga tataatctaa
    15961 taagggtaat tctttagatt atattaagga aatataggta tacaataaaa agttatggag
    16021 ttggattatg ggatataaat taattgtaac agatatggat ggaactttat taggcaataa
    16081 tcataaagtt acagatgaaa ataaaactgc actacaaaaa gttataaaaa gcggtattaa
    16141 tgtaacttta gcaacaggaa gagccttcga ttcagctaaa tgcaatgtag attttcttaa
    16201 agaggacatg ccaataatag cttgtaatgg ttcattaata agagagcaaa atggaaatat
    16261 aatatattca aataagattg atacaagaac atgtttaaac attttagatg tattagataa
    16321 atacgatatt tactaccaat gtaatagtat agattctatg ctttcgaaaa aaatagaagg
    16381 acgtgaagat agattaagtg tatttttagg ctctgaaaca gaagtaattg taaaagatga
    16441 tttgagagaa gaaatcttta aaaaagatat actaaaattt gtaatcatag aagaaaaaaa
    16501 tccatctatt ttggatgaaa taagaaaaga attgagaaaa gttcaaggaa taaagataac
    16561 ttcttcatgg ccaaacaata ttgaagtcat gaatgaaggt gttgataaag gtaatgcagt
    16621 taaaatttta gctgaaaaaa tgaatataga cagagaagat ataattgcat ttggagacaa
    16681 ttacaatgac atagaaatga taaagtttgc tggtcttggg gtagctatgg gaaatgcaga
    16741 agaattgatt aaacaagaag ctgattatgt tacagataca aatcaagaca gtggggttgc
    16801 aaaggcaatt tataaattcc tagagttaaa agaatcactt agctcttaaa acagtatata
    16861 ataggaaaaa ataggttgac tattttgtag atagtaatta taatacaaat agataaaaca
    16921 taaaaattta gattcagtga aaaagagagt agatatagag aaaatctcag cgaattgggg
    16981 gatggtgtga gcctaatgat aacactttat attgaaaaga actttggaga atactacttg
    17041 aaattcagta tggtagtagg taatctcgcc ttaagagaat agagtgggta aacattgttt
    17101 accaactaga gtggtaacac ggaatatttc gtctcttggt ctttttgacc aagagatttt
    17161 ttagtttcat tgagttacaa gacttataat taaaatacaa aaaattaaaa tatgaggtga
    17221 aagttatgaa attatggggc ggacgcttta gaaaagcgga aaatcaactt atggaagaat
    17281 ttaataaatc ttttggctat gattgtgtac tgtacaaaaa agatatcgaa ggaagtgtag
    17341 cacatgttca tatgcaagta aaatgtggac ttttaacaga agaagaagga aagtctataa
    17401 cagaaggttt aaaagggata cttgaagatg tagaaaatgg aaagttagta ttggatggag
    17461 agtatgaaga tatacatagt tttactgaga taaatttaat tcaaagaatt ggagatgttg
    17521 gcaagaagct tcatacagca cgtagtagaa atgaccaagt tgcagttgat atgagattgt
    17581 atgctaaaga aaaagcaaat gatttggtgg atttaatatc tgaatttaaa gctactataa
    17641 aagatgttgc tgacaaaaac ccagtgatga tgccaggcta tactcatctt caaagggcac
    17701 aagtagtgac atttaagcat cacttaatgg catattatag tatgtttgat agagatgaga
    17761 aaagaataaa aaatgctata gaaatattag atgaatcacc tttaggctgt ggagctttag
    17821 caggaactac gcatgatata gatagaagta taacttgtga acaattagga tttaaaaaag
    17881 tagtagataa ttttatggat ggagtaagtg atagagatta tttactagag ctaatgagtg
    17941 atttttcaat tataatgatg cacttaagta gattaagtga agagttgata ctttggagtt
    18001 ctcaagaatt tggatttgtt gagatagatg atttatatac aactggaagt agtataatgc
    18061 ctcagaagaa aaatccagat ggtgcagagc ttataagagg taagactggt agagtatatg
    18121 gaaacttatt tggtcttttt acagtaatga aaggaatacc attagcatat aataaagaca
    18181 tgcaagaaga taaagaaggt tttttcgata gtgtacatac tcttgaaatg tgtatacaaa
    18241 taatggatag aatgatagcc acactaaagg taaatgagga taaaatgaaa caagcagtaa
    18301 aaaatggttt tttaaatgca acagaagttg cggattattt ggttaaaaat aatgttgctt
    18361 ttagagatgc acatggtata gtaggaagta tcgttatata ttgtgaagat aataaaaaag
    18421 caatagaaga tttaacatta gaagaattac aaaagttttc agatgctttt aaggaagata
    18481 tttatgattt tatcgactat gagagtatat taaataaggg aataaaaaag aatttaaaat
    18541 aatgtattta tttttaaaag ctgtaaattt gaatgttatc atttaaattt acagcttttt
    18601 attgtgtaag attttattat
//
LOCUS       DAEORZ010000078        18591 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEORZ010000078 DAEORZ010000000
VERSION     DAEORZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.078"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            223..1383
                     /locus_tag="KRH39_002340"
     CDS             223..1383
                     /locus_tag="KRH39_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG8512239.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1725..1886
                     /locus_tag="KRH39_002341"
     CDS             1725..1886
                     /locus_tag="KRH39_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512240.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2079..2708
                     /locus_tag="KRH39_002342"
     CDS             2079..2708
                     /locus_tag="KRH39_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBG8512241.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2948..4207
                     /locus_tag="KRH39_002343"
     CDS             2948..4207
                     /locus_tag="KRH39_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG8512242.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4466..5461
                     /locus_tag="KRH39_002344"
     CDS             4466..5461
                     /locus_tag="KRH39_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBG8512243.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5707..6774
                     /locus_tag="KRH39_002345"
     CDS             5707..6774
                     /locus_tag="KRH39_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBG8512244.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKGKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7308..9218
                     /locus_tag="KRH39_002346"
     CDS             7308..9218
                     /locus_tag="KRH39_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG8512245.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9281..9904)
                     /gene="ytaF"
                     /locus_tag="KRH39_002347"
     CDS             complement(9281..9904)
                     /gene="ytaF"
                     /locus_tag="KRH39_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBG8512246.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10092..10736
                     /locus_tag="KRH39_002348"
     CDS             10092..10736
                     /locus_tag="KRH39_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8512247.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            10950..12008
                     /locus_tag="KRH39_002349"
     CDS             10950..12008
                     /locus_tag="KRH39_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBG8512248.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFRMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            12112..13296
                     /locus_tag="KRH39_002350"
     CDS             12112..13296
                     /locus_tag="KRH39_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG8512249.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTSSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            13503..14216
                     /gene="rpsB"
                     /locus_tag="KRH39_002351"
     CDS             13503..14216
                     /gene="rpsB"
                     /locus_tag="KRH39_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBG8512250.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14295..15206
                     /locus_tag="KRH39_002352"
     CDS             14295..15206
                     /locus_tag="KRH39_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBG8512251.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEICLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15291..15995
                     /locus_tag="KRH39_002353"
     CDS             15291..15995
                     /locus_tag="KRH39_002353"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBG8512252.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            16021..16578
                     /gene="frr"
                     /locus_tag="KRH39_002354"
     CDS             16021..16578
                     /gene="frr"
                     /locus_tag="KRH39_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBG8512253.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            16581..16784
                     /locus_tag="KRH39_002355"
     CDS             16581..16784
                     /locus_tag="KRH39_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512254.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTIKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            16813..17547
                     /locus_tag="KRH39_002356"
     CDS             16813..17547
                     /locus_tag="KRH39_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBG8512255.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPELVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELHKAIYVYQRRDRRFGGLK"
     gene            17560..18339
                     /locus_tag="KRH39_002357"
     CDS             17560..18339
                     /locus_tag="KRH39_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBG8512256.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         7638 a         1854 c         3230 g         5869 t
ORIGIN      
        1 taataaaata attgttgaaa aagattttaa attcagatta ttcatttcta aagtaattaa
       61 tgtgcaaatc tttattaata tatgtcaata gttaggtata tatgtattta attagcatga
      121 tatagataaa agtatttatt ttaattgtct attatctgaa aaagtcttta cttagaaatc
      181 ataaaaatca aattaattag gaaaagaaag ggtgtgtata atgtgattga gactggattt
      241 gtatattgta gtttcttggt tgtattcgta ggtgtaattt tttttatggt tgataaattg
      301 aaatggaagg tatttgatgt tattaatcca atgcttttaa tatacttagg ttcagctata
      361 cttggatcat ttggtttatt tgcacagaat gaagatgttg gaacgtatca aaacataata
      421 acagtgaact ttcttccatt aatgttagtg tttttgttaa ttcaatgtga tttaagaaaa
      481 atattaaaga tggggcctaa aatgttatta tcatttttct gtgctacatt tacatttatg
      541 cttggatttg ttatagcatt cataattttt aaaaattcaa tgggtgcaga ttcatggaaa
      601 attcttgggg cagcatctgg ttcatggata ggtggctcat caaatatgat tgcagcagct
      661 aatgctctag gagttgcaga agaaggactt agttatgcaa tacttatggg ttctatagga
      721 tatacagtat ggatgtctgt ttgtctaatg tctgtgaaat ttggacctaa atttaacaaa
      781 tggacaaagt gcgatacaag ttttatagat gaggttacat caaaacttga aaaagaaaat
      841 aaagataata cagcacctac gtttgtagaa cttatgattt tactttctgt tgggtttgga
      901 gttgcgtctt ttgcacaaat tgtatccaag atacttccaa cttcaggtat acttaatgaa
      961 actatgtggg tagtacttgt tgcatctaca gtatcaatat tattgggtat gacaaaaata
     1021 tcaaagctta aagggagtac actggtagga aatgtgttta tgtatttact ccttgcagct
     1081 acaggtagta aagcaaattt ctttggattg tcagaagcac ctgtttacat aatgtttgga
     1141 ttggtagtag tccttgtaca tgctatttca tttatagggc ttgctaaact atttaaactt
     1201 gatttattta cttgtgaagt aggaagtata gctaatatag gaggagtaac tacagctcca
     1261 atgattgctg gggttcacaa tcctgtatta ataccagtag gtgtacttat ggggcttcta
     1321 ggaaatatta tagggactta ttgtgctctt atggtaactc aaatattaca tatgttagct
     1381 taataaaaat atggtataaa attaataatt taaataagaa aagtcatttc aatttaagca
     1441 gaagctttta ctgaaatggc ttttttgttt gacaagataa tctatttcaa gaagatgttt
     1501 tgacatattt agtgtataat attgtaaaat ttactaaaaa cgagatatat ttaattttta
     1561 tattgaatcg aacgtctgta tgatgtataa taaatataca gaacgaatgt ttgcagaacg
     1621 ggtgtttttt gaatttattt taataattta tgaatatata tcagaatgca taaatatatt
     1681 taaatataaa gattaaaaag aatttaaagg agtgttgata acttatgata attccatcaa
     1741 gaagaataca agatataaca ttaacaaatt taaaaaatgg tgaagtaact ttaattgaat
     1801 tggatgaaat atacaagaag atgggatttt tatttgttgt tagtgaaggc aaattgaaaa
     1861 agataaaaaa ggagaatcat cattagaaat gaatgaaata ataaatatta taatagttta
     1921 taaaacctaa cttatgtaga atataaatac ttaagttagg tttttttgtg ataaagttta
     1981 taagtactca attttataag ataaataatg gtcttaagtg aaaagtaaat aagtttaaat
     2041 tttagtaagt attatattgc ataagaggtg atattattat gaaaatagta acctacaata
     2101 ttcataaagg tatggattca aataatagat taactctaac taaaatgggg ttatatttaa
     2161 aacaattgga ttgtgatgta atttgtttgc aagaagtatt atatcctcaa tttttagctt
     2221 taaaagctgt tttaaatatg gatggtgtct ttgcgacaaa tgtaaaaaaa gtaaatatga
     2281 tatatggtat ttgtacattt acaacatcta aaatgttaaa cagtaatcat tttttcttaa
     2341 ctagcaaaaa agaacaacgg ggagctcttt gtataactat agatgcatat ggaagaatta
     2401 taaatgtaat aaatactcac ttgggacttg ataggcagga gagagcaaaa caattagacg
     2461 aaataataga ctataggaac agattagtgg ggatagttat tttatgtggt gattttaatg
     2521 agaaaaatgt ctttttaagt atgtttaatg atatggctat ttcactaaac aagtcttact
     2581 taccaacatt tgaaaaatca aactctagaa tagattacat ttttgttaat aaaaatactg
     2641 aactaaaagg atacacagta gaaaaaattt acctgtcaga ccattatcct gtaataggtt
     2701 acatttaaat ttcaatataa atgaaattaa ttgttataaa ataaacaatt tttttgttaa
     2761 gaaatagtaa aagaaatata ataaaaaata tcaaaaataa aactaattat aagacataaa
     2821 tttacaataa agttacattt gattaaattc cttgtttaac aaaagctatt caaatataat
     2881 ctaaggtagt tttgaaatgg gaactctatt atgcaaattt aagctagtgt taaaatgagg
     2941 tgaagttatg ggcagaagaa caacaagagt ttcaagatta ggtaaagaaa aaaaacgaag
     3001 tgttttaatt gtattggcag ctttagttct aataatgggt ggatttactt atttttttaa
     3061 cagtaaattt ctgtacaatg ggaaaatcgc taaaaatgta tatatagaag gagtaaatgt
     3121 atctgatatg actaaagcag aggcattaaa agctataaca gataagtata ctccagaaga
     3181 tttaaatctt gcttatgatg ggaaaaaata tacaatatca ccaaaagata ttgatttaaa
     3241 atatgataca gaagatgttg taaaagatgc ttatgaaagt acaaaaaaag ggtcctattt
     3301 tcaaaactta aaaaaatata ttgatataag agtgaataag gcaaatatga agataaaagc
     3361 tgaatatgat gaggcaaaac ttagttccaa agtgtcttct atagcagata gtataaatgt
     3421 taagatgaaa aatgcttcta ttagtgttgg aagtggtgga ctaagttata cagactctgt
     3481 tgtaggtaga gaatttgatt tggcagcaaa taaagagtct atatataata tgataaaaaa
     3541 taaagagcat aaaactttag aacttaaagt aaatcttcaa aaaccagata taacaactga
     3601 acaagtaaaa actgtaaatt ctgttatagg tcaatattca actacttatt cacaagctgt
     3661 agagggtagg tcatataatg taggattatc agcaagaaaa actagtgatg tattattaat
     3721 gccaggtgaa gaattttcat acaataaact tacaggacct agtaataaag caaatggtta
     3781 caaagatgca cctgttatag tttatggtaa gctagaacaa tcagcaggag gaggtgtttg
     3841 tcaaacatct tcaacagttt ataatgcggc tcttctttcg ggtatggaga taactcaagt
     3901 tacaaatcat tcatctgcat ctacttatgt gcctaaaggg agagatgcta ctgtgagcga
     3961 tggaggatta aacttaaagt ttaaaaatcc atataagcat cctgtatata ttaaaaatta
     4021 tgcaggtgga ggaagtgtat catcagtaat ctatggtaat tctggtgaca aaccaaatat
     4081 atctatagag gtaaaacaaa ctggtcagaa taaatattct acatatcgaa tatttaaaga
     4141 ttcaagtgga aaggtcataa aaaaagaaca tatatcaaat agttcttata aagaattaaa
     4201 aaaatagata attttttaga gaaatatttg taatataaaa gggagtgtct caaaatgaac
     4261 tttttagttt atgagacact ctttttctgt gaaatcaaat atattttcag catttcaaca
     4321 atacaaaaga agcgtatctc tattttgaga cagccttttt tgtaaaaatt aacactatta
     4381 gttcttgttt taatattaca atcattaata tatagaaaat attcctatat attggtataa
     4441 ttgtaaatag atttagttat ataaaatgta tgaggaggtg acatggttga attttatagg
     4501 caatagatat gaggtagtaa attctgatga cgttttagaa attaacaaaa tctataaagc
     4561 tagagatgtt ttttacagga aaaatgtgtt gataaaagtt ataaagcaca ataattatat
     4621 ctgtgaagat tttgtttcaa atttaattga tgaaagtact gctttagatg agataaactc
     4681 tccttatata ctaaaaataa tagatgttgg gattcactgt acagaagaaa caacattata
     4741 ttatgttgta agtgaagact ttaatggtat cggactagat gaattaattc taggtaatta
     4801 tcttcattta gaagcaattg taaacataat gatacaagtt ttaaaagctt tagaaatgat
     4861 acataggaat cattcatatc atggtagttt aaaatcgagt agtataatag tagatacaga
     4921 atacaatata aaaatttgtg attttggaat tacaaaagca aataatgggg ttaatacaag
     4981 aagctatgga aacaggagat atttatgccc acatcaacta tgtataaatt atactgataa
     5041 agaaagtgat ttttttgcaa caggtcttat tctatttgaa tctatattta aaaaattgcc
     5101 ttttggagaa tcaaattcag aagaaaaaat gctacaatct attgataagg gattagattg
     5161 gaatagtata aaagcaatta atggaaatac tgaacttata aatgttataa aaaggcttct
     5221 tggaagaaat aataagtata ccagagccaa tgatattatt attgatttaa gtaaagtaat
     5281 gtatgaaaaa gcatatatag aagttgaaga tgagaaagaa gaagagcaga aacaagacaa
     5341 agttatacaa gttaaaaaag aatataaaag taaaatgaga aaaaaattac ataaaaaact
     5401 tgttgttgcc ggtctagcta tagtcatgat ttcaatgatt attattagtt ccataatata
     5461 aaaattgtat attaagtatt ttaaaatcaa tgtctataaa ggatatacat agaaatttta
     5521 gtgtagtctt aaattatgaa aggaagtggt tattatgtta acaaaaaata atgaggtatt
     5581 aaacgtctag gagtatttta acaaatagcc acctgctttt acagtttaga ataaaaaagc
     5641 tgtggctaaa ggtcacagct ttttgctact ttttaaaggc aggagaatat taaaaaggag
     5701 caaattatga gcaatataaa aataatagca agtaataaat gttggattga agatatagca
     5761 aggaaacaac ttgaagatat ttcaaagtta aatggaattt taagagtagt aggattacct
     5821 gacttacatg caggtaaaat acctgtaggt ttagcagttg agactaaaaa tattatttat
     5881 ccacatataa ttggaaatga tataggatgc ggaatgactt tatttaaaac tggggttcta
     5941 aaaaagaaat ttaaaaaaga caaatggatt aaatcactta gtaagattaa agatttgtct
     6001 gatatagaaa taaaaaacac atataaagaa gaatgtccaa ttttaaattt aggaactata
     6061 gggagtggaa atcattttgt agaaattcaa tgtataagtg aaatttataa taaagaacaa
     6121 tttgaacaat taaaattttc atctgatgat attatgatgt tagttcactg tgggtcaaga
     6181 aattatggag aagatatttt aaaaaaattt tatgataaag atggacttga agttgaaagt
     6241 gagaaagcta tagattatat taaaaaacat gataatgctt taatgtgggc agagcgtaat
     6301 cgtgaagcta tcagtaaaaa aattatgcag tcaataggta catctggaga tgttgaaaca
     6361 atattctcta taaatcataa ttttatagag aaaagggaag ataaatttat acatagaaaa
     6421 ggtgctgttt caagtgaaag aggagctgtg attataccag gttcaagagg cagtctatca
     6481 tatatagtta tgcctactga aaatacagag atatctttat attctctatc acatggagca
     6541 ggtaggaaat ggtctcgttc agtttgtaaa tctcgcctta aaggtaagta tagtaaagat
     6601 actataaaac aaactaaatt taaaagtcag attatatgta atgatgtaaa tttattattt
     6661 caagaagctc cagaggcata taagaatata gagcagatta ttgaaagttt gatagaatat
     6721 gaacttattc aagtagtggc aactatgaag ccattgatta cttataaagg ataattttta
     6781 aagttaaaaa agattaaaat tatattataa atagctaatt ttatagaaca tttaaaagtg
     6841 ttttattatt agctattttt ttattttgta gagacgataa tttatagagc taaattcttt
     6901 gatatataat gattacaaag ttagactgta ttggaaaatg cacatacatg aattgatgac
     6961 aatataagtg attcccaaat ataaatgaaa gtataagctt attttcacta taaaaatttt
     7021 cactgtacaa aaacattaat gacattattt ttattgacat aaatatataa tttatggaat
     7081 aatattagaa gcactgatat aagtgtttaa ataagaatag aaaaggagaa gtgttttatg
     7141 aaaaaaaata ctaacattaa tgagtccgga gttaaacttc ttaataaata aggagtttga
     7201 tatataataa ctccttattt attaagttgt tcgcaaaata actattatca aagtaaatga
     7261 tagggtgatt taaaagacga tgacttataa ataaaggaga aaaaataatg aatagtaata
     7321 catttgaaag attacaacta aatgaagtta aagacttaat aaaaatacat tgtgtaagtt
     7381 cgctaggaaa aaatttaata gataaattaa ctcctagtgg gaatattgga gtagtaagaa
     7441 ggaaattagc agaaaataaa gaagctagaa agataattga aaatagtaat cacataccac
     7501 ttgaaggatt gtttaatgca ggttctacaa tagataaaat tgaaaaagga atgataatag
     7561 aacctgttga acttgtaaat attgaagatt ttttaagagg ttgtaggaag atgaaagctt
     7621 ttatgttaga aaaagaattt tattcaccta cattaagttc ttatgcctta aatataacag
     7681 aatgtaaaag tatagaagat gagataaatt actgtataaa atctaataaa gttgattcta
     7741 atgctagtaa agaattaaaa aagataagac gaaatataga aattacagaa ggaaagataa
     7801 aagatagatt aaataaattt ataacatcaa ctgtaaataa aaagtatata caagagttta
     7861 taataagtaa aagaaatgat agatatgtaa taccaattaa atcttcttat aaaaatgaag
     7921 ttaatggtac tatattagat acatcatcaa aaggaaatac ggtatttatt gaacctatta
     7981 gtgtttcaaa tttaagtaca gagttaacta tgttaaaagc tgatgaaact attgaagaat
     8041 acaaaatatt atcttattta acagaactta tatttgagaa gataagtcag ataaaattaa
     8101 atatagagat actttcagaa tacgatatgg tatttgcaaa agctaagtat agtcaaaaaa
     8161 taaaaggaat tactccaaaa ataaataata atggctatat aaaaataatt aaaggtaaac
     8221 atccacttct aacaggagat gcagtacctt tagattttga aataggcaaa aattatagaa
     8281 gtcttataat aacaggtcct aatgcaggag gaaaaactgt aacccttaag actgttggtt
     8341 tactaacttt aatggttcaa tgtggatttg atatttctgc aaaagaaggt agtgaattta
     8401 gtgtatttga aaaagttttt gtagatatag gggataatca aagtattgaa aatgccttaa
     8461 gtactttttc atcacatata aaaaatattg cagagattat gagtttatca aataattcta
     8521 ctcttgttct gtttgatgaa ataggaagtg gaactgaacc taatgaagga gctggtttag
     8581 caatatcatt attagaagag ttttacaaaa tgggctgtat aactatagca tcaactcatt
     8641 atggagagat aaaaaagttt gctacattgc atccagaatt tgaaaatgct ggtatgatgt
     8701 ttgataaaga aacgttagaa ccattgtata agttaactat tggaaaatct gaagatagta
     8761 atgctctttt tatttctaaa aaaatgggaa taaaaaatag agttttagat agagctaaag
     8821 aatatgtaat tgatagaaac tacaatttag atttagttaa aagaagtaag ctacaatcta
     8881 atattgaaga taaaaatagt tctgaagtca ttttaaacta tacagattat aatgtagggg
     8941 ataaggtaaa attactagat gaagatgatt ttggaattgt atataagcct atggacaagt
     9001 ttaataatgt agaagtattg tttaatgata attttatttc agtaaatata aaaagattac
     9061 aacttagcat aaaagcatca gaattgtatc cagaagggta tgatttagat gtattattta
     9121 ctagttttaa agaaagaaaa ttagaaaaag atattcaaag aggttcaaaa aaagctctta
     9181 aaaagattca gaaagaaatt agagagaata gaaaataaat ataaaaagca caaaagaaag
     9241 caatctaaag agaaaagttt atctttagat tgctttctta ttaacatatc ttcataaatc
     9301 caatgactaa caagattaaa ccacttaacc aagataagtt taaatttacg tttttaacaa
     9361 acatatttcc tagtaaacaa ccaagtaata ctgcaattat atttgatatt aaagaaaata
     9421 gaataacttg tatataattc acacctacaa ggccactacc aaaaccgata gctaatccat
     9481 ctaagcttaa agcaagtgca agtgtaaatg cttcagcaga tttaagcact ttagatttac
     9541 ttgtatctaa gacatcaggt tcgatactta cattaaaatt taatttaagg tctaacatat
     9601 taaaattata attctttgag tgtatagatt ttttcttaaa ataagattta aaaaaacttt
     9661 caattagtct agcacttcct aataaaaata aaataaagaa acatattaac attgttatat
     9721 tttgaggtaa aaagtctcta actattgtac ctgcaaaaag cgatattgct aaaatagctg
     9781 aacaaactac gcttataatt atatttgaat ataagggaac tttaacttta tttacgccat
     9841 atccaaagct tgtcacaaag gcatctatac aaagagaagt aactaataaa attgattcaa
     9901 tcacagtaga ttaacatcct ttctcttaaa ttttgtactt atattagtta tagtattgta
     9961 aaaatggata aattgttaca gataatccaa taaattgttg ataaaatttc aacatcatac
    10021 aacacctaaa taacaaaaga atcatatcat ataataatat aaaaccaatc ataataaagc
    10081 aggtgaaaat aatggaacaa catagtaagg ttgggaattt cggtgatttg ttgaaagttt
    10141 tattagatga aaaagaatta tcaatgggag aactaagtaa aaaaagtgga attgataaat
    10201 ctactatatc tcgtatagct aataataaac aaaagccaaa tataaatcat ttagaaaaga
    10261 tggcaataca tttaaatatt aacctagaag aactgttgaa agcttcagga tatgaattta
    10321 gaaacagcaa taatcaaata tttaatgcag atagtgattt tagtaacttt gatgatattc
    10381 ttggatttgc aaatttaatc aatgataaga actttaatgg aaatattgaa aaagagttaa
    10441 gtaagtgtaa gttatatgtc caaacagatg aaggtaaaaa attacttttt gataatttta
    10501 ataaaaaaat agattctatt gaaaaacaag gtcaatttac agatagatta agacaaatgt
    10561 atgctgattt ttgtacagat ggacttacaa taaagaaata tttattgatt ggaagtatgc
    10621 tactttactt tgttatatca acagatgtga ttccagactt cgtgttccca ataggattta
    10681 tggatgattt agtagcatta aatatagtta caaaattact taaaaatgat aagtaagttt
    10741 tagaatcata tttaatatat aaataaatca aagtatataa aatttattat tttttatatg
    10801 aaacaaatta aaagcacaat gaagtagatt aaaaatttaa aactaagaat ctaatatata
    10861 ttttgtaact atttaagtat ataaaataaa ataatatcca aactaataca aaatagtaat
    10921 aattgttttg tactggagga tatatattta tgaattgtaa atggatatct aaaatagatg
    10981 agagaaaaaa atgtcataga gaagcagatt cttctggata ttgtatcttt cataaagaaa
    11041 ataagtctga tgaagagata cagttgatga tggatactct tcataaagaa gagataagtg
    11101 aatttaatgg atttgttttt gaaaatgaat ttaatgcgga agaaatatta acttacaatt
    11161 ataaaatact ggatttttct gaatccatat ttaaacaaaa agcaaatttt aaaaaatata
    11221 tttttaaaaa gaatattatt tttaactata cagaatttag agataaagtt ttatttaatg
    11281 gttgtgtatt cttagaaaac tgtgatttta atagaaccat atttagtaaa cactatataa
    11341 atgaccgtat atttgaaaaa gtgaaattta aaggacctga tttggtagtt aataaagtag
    11401 aaaattttcc acgaatggat ggaataattt ttagcatgtg tacaaaattt gtgttaaaga
    11461 atgttgaata tggaaaaagt gagtatgagc atggcaagat aaattataga atagctagaa
    11521 atcaagcaac aaaaattgga gaatatgaga tgataggatt ttactattat aaggaaagaa
    11581 tttatagtag taaaataatg aaacgttcta attatcctac tttcagtgat tatttagttg
    11641 aaaaattttt tgaccaaata gcacgttaca caacaggtta tggagaaaag ccatggaata
    11701 tattattagt tattatagca ataataagtg tttttgcttt attatactta tttgtaggca
    11761 tagaaagttc aaatagcaca cttgtagctt tagatataaa caatataggt gattactcac
    11821 taagtgagat tttcagaatg tatatggatt tatggtattt tagcatggct acatttagta
    11881 cagtaggata tggagatatg gttgctacaa gtttgattgg gaaagcattg gcaggtatag
    11941 aagtattttt tggagttaca ataggagcaa tttgggcatc tgtgattatt aaaagaatga
    12001 taaggtaata tttgtgtaaa tatggatagg tattaattac tagatataaa ttggactaaa
    12061 tagggaaaat ttaaatataa atatacttaa taatccaaga aggtgaaaaa tttgaaaaat
    12121 gcactaaaaa aaattatatc attagctatg attctagttt ttataatacc aaattattca
    12181 tatgcagatg ataaatctgt agacaagtat tcaaaaacat ctatacttat agaccaagaa
    12241 acaggtagag ttttatatag taaagaacct gatatgaagg taccattagc tagtttaagt
    12301 aagatgatga cttttttact tgcaatagaa gctattgaaa ataaagaagt aaaagaaact
    12361 gataaggtaa aaatagacaa atctattgca tcagtaaaag ggtcaagtta taaacttaaa
    12421 gatggtgaag aagtaccact tatagagctt atgagaggtc ttatgattgt atcaggaaat
    12481 gatgcggcaa ttgctatagc aaaacatatt tgtaaaacga aagaagcatt tgtagataga
    12541 atgaataaaa aagcaaaaga aataggaatg agtaatacac attttctaaa tccaaatggt
    12601 cttccaatat acgatttaag caaccccaaa aaaccagcaa aagaaaatat atctactgca
    12661 aaagatatag ctattcttgg aaaatacatg tttgaccatt atgaaaaaca agtaacagct
    12721 ataactgata tggagacata tacaaattca gatagaagtt ttgaaaaaag caatacaaat
    12781 gcattattaa gaattatacc agaagtagat ggtataaaaa ctggatatac tggcaatgct
    12841 ggatattgct tatcattttc tatggttgta aataaaaatg ataaaaatga gaaaaaacgt
    12901 agagtaatag gggttgtatt aggtactaac cataaaaata aaagaacctc tgcttcatta
    12961 gctctactga aatatggaaa agaaaatttc aatatgaaaa aagtaattga taaagatagc
    13021 tttattggta agaaatatat aaaaggaatg aaagagttag aggttacatt aaaagcaaaa
    13081 gatgaacttt atacaatatt aaaagatgat gaaagtataa aatcagaagt taagattaaa
    13141 aatatagagt accctgttaa aaaaggcgat actatgggta ttataaaata ttatactagt
    13201 tcaggtgact tacttggaag tgtagatata gtaagtaata atagtataga taacgtatca
    13261 ttaaaaacaa aattaaaaat gaaatttgca aattaaattc aaaaaatggt ataaaataca
    13321 caaaaaaata taaaaataga aaatatggaa aaaatattgt tatttcttca cctctgtgat
    13381 atactactag aggtgaaaaa ataaaataaa tcacacacag cattgggatc cttaaaaggt
    13441 gcctattaca tggtttttta gggagaagat caagctggag gtataaaacc aggaggtaag
    13501 atatgtcagt aatatcaatg aaacaattat tagaagcagg tgttcacttt ggtcaccaaa
    13561 caagaagatg gaaccctaaa atggctaagt atatattcac agagagaaat ggaatatata
    13621 taatcgactt acaaaaaaca gttaaaaaag ttgaagaagc ttacaagttc actaaagaag
    13681 tagctgaaac aggaaagcca atcttattcg taggaactaa aaaacaagct caagatgcga
    13741 taaaagatga agctgaaaga tgtggaatgt actttgttaa tgaaagatgg ttaggtggaa
    13801 tgttaacaaa ccacaaaact ataaaaacaa gaataaataa attaagagaa ttagaaaaaa
    13861 tggaagaaga aggcgtattt aatgttcttc ctaaaaaaga agttataaaa ttaagagctg
    13921 aaaaagaaaa actagaaaaa tacttaggtg gaataaaaga catgcctgaa ttaccaggtg
    13981 caatgttcgt agttgaccca agaaaagaaa acatagctat tcaagaagct catagattag
    14041 gtataccagt agttggtata gttgatacta actgtgaccc agaacaatta gactttgcta
    14101 taccaggaaa tgatgacgct ataagagccg taaaattaat aactggtgct atggcaacag
    14161 ctgtaattga aggtagacaa ggtgctgaag aagaagtagc tgaagaccaa gaataataat
    14221 ttgacaatta aatggtaagg gtcgccctta ccatttataa tatggaatag aataagtagg
    14281 aggaaactta aaaaatggct aatataactg ctcaaatggt aaaagagtta agagaaagta
    14341 caggcgcagg aatgatggac tgcaagaaag ctttacaaga agctgaagga aacatggaaa
    14401 aagcagtaga tttattaaga gaaaaaggat tatcaaaagc tgctaaaaaa gctggtagag
    14461 ttgctgctga aggtctagtt gcaatagaaa tgaatgatga taatacagtt gcttctatgg
    14521 tagaagttaa ctcagagaca gatttcgttg ctaaaaacga agacttcaaa gtgttcgtta
    14581 aagatgctgc ttgtatggca ttagctactg ataaagaaga tatagcttct ttattaggtg
    14641 aaactcataa agagggaata actttacaag aagttttaaa taatagagtt gcaaaaatag
    14701 gtgaaaaatt agactttaga agatttgcta aagttgtaac taatggacaa gttgctggat
    14761 acattcacgg tggtggaaaa ataggtgttc ttgttgaaat ggaaacagaa gctagagatg
    14821 ctaaagtttt agaattaggt aaagatgtag ctatgcaagt tgctgctatg aatcctaagt
    14881 atgtttcaag agatgaagta gatgctgaat acatagcaca tgaaacagaa gtattaactc
    14941 aacaagcttt aaatgaagga aaaccagcta atatagttga aaaaatggtt aaaggaagat
    15001 tagaaaaaga attaaaagag atttgtttat tagagcaaac tttcgttaaa aatccagata
    15061 taactgttaa acaattagtt gctgatgttg ctaaggcagt aggttctgat ataaaagttg
    15121 ttaaagttgt gagattcgaa gttggtgaag gtatacaaaa gagagaagaa aactttgcag
    15181 aagaagttgc taagcaactt aagtaataac caaagagaga acacgcaggt gttctctttt
    15241 ttaaaaaata gaaaaaattg gatatgaagg attgaaatag gaggtttctg atggataaac
    15301 caatgtacaa aagggtattg ttaaaattaa gtggagaagc attagcagga gaaagaggtt
    15361 ttggtataaa taatgatgta gttaatgata ttgctattgc tataaaaaaa atacaagaaa
    15421 taggtgttga agtagctgta gtagtaggtg gaggaaactt ctggagaggc agaaccagtg
    15481 aaggaatgga cagaactact gctgactata taggaatgtt agctacagtt atgaatgcaa
    15541 tggcgctaca ggatgcacta gaaaatatag atgttgctac aagagttcaa actgctatag
    15601 acatgagaca aattgcagaa ccatatataa gaagaagagc agttagacac ttagaaaagg
    15661 aaagagttgt tatatttggt gcaggaacag gaaaccctta ctttacaaca gatacaactg
    15721 ctgctttacg tgctgctgag atggaagcag aagttatatt gttagctaaa aatgttgatg
    15781 cagtatatga caaggatcca aaagtacatg cagatgctaa gaaatttaca gaattaagtt
    15841 acatggaagt tatacagaaa gaattaaaag taatggattc tacagctaca tctttatgta
    15901 tggataataa aattccaata aaggtatttg agcttacaac agagaatata ataagagcag
    15961 taaaaggcga aaatatagga actactgtaa aataatgaag aatgaaggag gagtaaaatt
    16021 atgaaactgg aaatacataa gcaattagaa gagaaaatga atgggacaat tgatgcttta
    16081 aagtttgaat ttggaactat aagagcagga agagctaatg cacaaatgct agacaaaata
    16141 agagttgatt attatggaac tccaactcca ataaatcaaa taggtgctat atctgtacca
    16201 gaaccaagaa tattaatgat atctccttgg gataaatctg ctatgcatga aatagaaaaa
    16261 gcgatagcta actcagactt aggattaaat ccatcaaatg atggtgaggt tataagactt
    16321 tctgtacctg ctttaacaga agaaagaaga aaagaattag ctaaaaaagc tagtaaagct
    16381 gcagaagaat ttaaagttag aataagaaat gaaagaagag acgccaatga gaaaattaaa
    16441 aagatggaaa aaggcggaga attaactgaa gatgaattaa aaaaagcaca agatgaagtt
    16501 caaaaaatga ctgataaatt tataaaagaa atagatacat tattatctaa aaaagaaaag
    16561 gacataatgg aggtataata ttgaaaaaat attataacct tttaggtctt catgtagatg
    16621 aggtaaaaca atattttgat gagcaaaata taaattatac cataaaatct attgaaggta
    16681 aaaaggataa agaaaaactt gtagttccta gagttataaa aatatctgag ataggtgata
    16741 gtgtagaact gataatcaca tacttttctg actccttagt ttaaggagtc agaacaatat
    16801 tggaggcaaa atatgaataa caacattata tatgatatag acttaaataa catacctact
    16861 catattgcta ttataatgga tggaaatgga agatgggcaa aagctagatt tcttccaaga
    16921 actgcaggac ataaggctgg agtagaaact ataagagata tagtaaaaga atgctcaaaa
    16981 ttaggtgtta agcacttaac attatatgca ttttcaacag aaaactggaa aagaccgaaa
    17041 cttgaagttg acacacttat gaacttatta tctacatact taagaaatga aatagctgaa
    17101 cttcaccaaa acaatgtaaa agtaactgct ataggagata taagtgcttt accaaaaact
    17161 tgtataagag aattaaactc agctaaggaa ctgacaaaaa acaatacagg agttaactta
    17221 aatttagcat taaactacgg aagtagagca gatataaaaa atgctcttat agatatagta
    17281 aaaaattgtg aaagtggtaa aatagatata aacaatattg atgaagatat tataaaaaac
    17341 tatttaagca caaagtctat accagatcca gaattggtaa taagaacaag tggtgaacaa
    17401 agattgagta actttttact ttgggaagtt gcttattctg agttttactt tactgatata
    17461 cattggcctg attttaaaaa agaagaacta cataaggcta tatacgtata tcaaagaagg
    17521 gatagaagat ttggaggact taagtaagga gattatttta tgcttacaag aattattgct
    17581 tcactagctc tggttccatt atttctattt gttgtatatg ggggtattcc tctatatata
    17641 gctgagacag caatagttta tattgcattg catgagtttt ataaggcatt taaaataaaa
    17701 gatgtacatc caatattcat tataggatat ttattttcta tttatttagc tgtaaaaaat
    17761 atttttaatt taccattaga atatacttat gcagtaattt ttattttatt tttagcaagt
    17821 ataatttata tgttgatggg caaaaacaat gttatagatg tttcgattac gtttttagga
    17881 gttttttata taggagtatt tttagatttt ataattataa ctataaatgg atttgaaaaa
    17941 ggtagtatat atgtatggct tatatttgtg atatctttta tgacagatat atttgcgtat
    18001 tttagtggtt atttacttgg aaaacataag ctaattccta aagttagtcc taaaaagact
    18061 atagaaggag ctattggagg gataatagga agtacacttt gttgtatttt atttggatat
    18121 ttatttggca ttgatttgct tcaattagcg ataataggaa gtataggaag tgtaatagct
    18181 caattaggag atttatttgc atcttctata aagagatatg tagggataaa ggattatgga
    18241 aagataatac ctggtcatgg gggtatatta gatagatttg atagtgttat attagttgca
    18301 ccttttgtat atagtgcgat taaatttttt attagataaa gattggttgg atttaaaaga
    18361 gcaatctata attgctagta atgaaaatag ctatgtaact tttaaatgca gagatattct
    18421 gcagttatta gttacatggc tattttcatt gttcaaaaat ttagaagacc ttggggaatt
    18481 gtataagaat atgtattact ttataatagt atactatatt tgtagtgagt atatttttta
    18541 tactataact atcattaaaa aatatttttt tgtttaataa tttcgatatt t
//
LOCUS       DAEORZ010000079        18547 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEORZ010000079 DAEORZ010000000
VERSION     DAEORZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18547
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.079"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            1..2178
                     /locus_tag="KRH39_002358"
     CDS             1..2178
                     /locus_tag="KRH39_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG8512257.1"
                     /translation="MYNKCNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLT
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALHSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANVMNIDDDLLKAGYEASIRHMFKKTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDNEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYRESDINSIIDDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2192..2614
                     /locus_tag="KRH39_002359"
     CDS             2192..2614
                     /locus_tag="KRH39_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8512258.1"
                     /translation="MKSLFKFNRELCTACGACLLACIDQNDNNIDNGEIFRHVFTIEE
                     GESIEYFSLACIHCNEPKCLDVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCVERYKYGMDSPCVMVCPTGALKFNK"
     gene            3021..3824
                     /locus_tag="KRH39_002360"
     CDS             3021..3824
                     /locus_tag="KRH39_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG8512259.1"
                     /translation="MKKLKLIIMLALVSALLITGCSSGNNKNKEEQKTIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMVEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMHKDGTLTKISEAWLGKDITKEPINE"
     gene            3817..4485
                     /locus_tag="KRH39_002361"
     CDS             3817..4485
                     /locus_tag="KRH39_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG8512260.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     GKAY"
     gene            4721..5383
                     /locus_tag="KRH39_002362"
     CDS             4721..5383
                     /locus_tag="KRH39_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG8512261.1"
                     /translation="MIFNMKYFLELFPIIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMNDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDKGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5443..6240
                     /locus_tag="KRH39_002363"
     CDS             5443..6240
                     /locus_tag="KRH39_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8512262.1"
                     /translation="MIKRNIIKCLVAVLSLTFVVAGCSKKEEVKDEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVAASVNKD
                     NLFEEKVYGDGTNGLQDLDLGRVSAWIDAELTVVGDAKKANMKVRVLDEKLSVTNIAY
                     FFKKNDEKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            6373..7548
                     /locus_tag="KRH39_002364"
     CDS             6373..7548
                     /locus_tag="KRH39_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8512263.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEFKTSKLIKEE
                     LNKLKIEYIDVAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKILENIV
                     RNVCRAYGADCKFEKSTGGDAVINDKNMIELGRESACKILGVENVEIIDFPAMTGEDF
                     AIYMKEKPGLFMYIGVGNKEKNINYRLHSNKFNIDEKCLSIASSLFSQLAVDYLCQL"
     gene            7657..8406
                     /locus_tag="KRH39_002365"
     CDS             7657..8406
                     /locus_tag="KRH39_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG8512264.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEINQSEVVAILGPSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDIANLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSNKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIVRKEEVFS"
     gene            8997..10058
                     /locus_tag="KRH39_002366"
     CDS             8997..10058
                     /locus_tag="KRH39_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG8512265.1"
                     /translation="MRDFLKNYKSSIILLACTIIGAIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKSTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKSLMVAQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     VGTAIAMMGDRGNTIRKLLSEGTEAILKVVSIVMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAAMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGSVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
     gene            10071..10268
                     /locus_tag="KRH39_002367"
     CDS             10071..10268
                     /locus_tag="KRH39_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG8512266.1"
                     /translation="MLVVSIFGFPVEAIPLLTVITTITDIPNTILNTTGNTVSSMLVS
                     RLVEGKDWLIDKTAITTKKIS"
     gene            complement(10432..11136)
                     /locus_tag="KRH39_002368"
     CDS             complement(10432..11136)
                     /locus_tag="KRH39_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG8512267.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSNELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSTCVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHKFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFCFQK"
     gene            11301..12026
                     /locus_tag="KRH39_002369"
     CDS             11301..12026
                     /locus_tag="KRH39_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8512268.1"
                     /translation="MMRKIIKIDEGKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVETKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMVPWQIVTISVDGKIVDGEI"
     gene            12086..13678
                     /gene="hcp"
                     /locus_tag="KRH39_002370"
                     /gene_synonym="priS"
     CDS             12086..13678
                     /gene="hcp"
                     /locus_tag="KRH39_002370"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBG8512269.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEIEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDKLLGIVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKAIEL
                     GGYKEDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            14052..14957
                     /locus_tag="KRH39_002371"
     CDS             14052..14957
                     /locus_tag="KRH39_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG8512270.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMTNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LISLGENCDIKDAFGWIKKKSFYRN"
     gene            15081..16034
                     /gene="msrA"
                     /locus_tag="KRH39_002372"
     CDS             15081..16034
                     /gene="msrA"
                     /locus_tag="KRH39_002372"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBG8512271.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVISGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESKEHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNDKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            16187..17317
                     /locus_tag="KRH39_002373"
     CDS             16187..17317
                     /locus_tag="KRH39_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8512272.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLFNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVNLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     NLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(17434..18402)
                     /locus_tag="KRH39_002374"
     CDS             complement(17434..18402)
                     /locus_tag="KRH39_002374"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBG8512273.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPNTYSNLDLS
                     SMVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
BASE COUNT         7227 a         2028 c         3262 g         6030 t
ORIGIN      
        1 atgtataata agtgtaataa tttgtattta ttattaggaa aggaaaatgt taatatggat
       61 agttatagac aacttaaagc taaaatacca tgcaaggaaa caggtataga aataaagagg
      121 actttatgtg atatttgttc tccaggagta cattgtggaa ttgatgcata tgttaaagat
      181 ggtaaagtaa taaaaataga aggtacaaaa gaacatccaa tgaataaagg attgttatgt
      241 accaaaggtc tttgcaatag agaatatatc tatagagaag atagaataaa aacacctcta
      301 aaaagaattg gaaaaagggg tgaaggaaaa tttaagccaa tatcatggga tgaagcttat
      361 agagaaatag cagaaaaatt aaatagagta aaatctgagt atggagcaaa gcatgtagct
      421 ttttttacgg gttatggaaa gtggtataga ccgtttttac aaaggtttgc atcttcattt
      481 ggttcaccta attatggtac agaatctagt tcatgttcaa catcagcaca aatggctgga
      541 aatgttgcta ctggaagaaa ctttacaatg caagatatgg agaatgcaaa aacttattta
      601 gggtggggat ttaatccata ttactctaaa tttttaaata gtgtgaatct aacaaaatta
      661 aaagaaaaag gtctaaaatt tataattgta gaccctagaa taacaccgac aactcaaaaa
      721 ctagcagatt tacatttaag aattaaacct ggaactgatg gagctttagc tcttggaatg
      781 gcgaatctat ttatacaaaa ggatttaata gatagagaat acatagaaaa gcatgtacat
      841 ggatttaatg aatataaaga atatgtaaaa ttatttgatt tagatactgt aagtagaatt
      901 acaggagttt ctaaagataa tatagtaaaa gctgttgaga tgttgtcaca aggtccattt
      961 tgtataaatg aaagtgcagc tccaatagtt catcatcaaa atggattcca aaactataga
     1021 gcaatgatgt ctttatctgc tataacaggt aattatgata gaataggtgg aaacatacca
     1081 aaacaagatt actttgctgg taagataggt ggattccaaa cactagaaaa agagtttgta
     1141 aaagaagttc tacctaaaaa tataaatgaa agaattggtg gagaaagatt tcctctatgg
     1201 aatgaaatgg tgactcaatt tcaagctatg gatttaccta ggcaaataga agaaaagaca
     1261 ccatatgaaa taagagcttt gttttgtcat gggataaatg ctagaatgtt tcctaattca
     1321 gggagacttt ttaaagcgtt gcatagtgta gatttttttg tgaatataga tttatttatg
     1381 acagatacat caaaatatgc agatatactt ttacctgcgt gtacatcatt tgagagagaa
     1441 gaatgtaaga gttatccagg agggtatatt acatatacca aaaaagttat tgataaatta
     1501 tacgattcaa aatcagatgt agaaatactt tcagatttag caaatgttat gaatatagat
     1561 gatgatttat taaaagcagg ttatgaagca agtataagac atatgtttaa aaagacgtgt
     1621 gtagatgtag ataaacttaa agaaagtgat ttaccattaa aatgtaaaga ttttaagcca
     1681 tatatagtag gctcatatac aagagaaggg tatgatacta gcacttctag gtttgaatta
     1741 aaatctacaa ttattgaaaa atataaagat tttggactgg atgcactacc aacatataga
     1801 ggatttaatg ataatataga taatgaatat actcatttat tgacatcagg agcaagaatt
     1861 tcaaatgcac tacattcaag acttcatgac atcaaacttt caagaatatt aaaaaatgaa
     1921 gctagttgtg atatttccag cttagatgca aaagaattgg gtgttatcag tggagattat
     1981 ataaaaataa ctactaagat aggaagtttg gatgtaaaag tcaatgttac tgatagagtc
     2041 ttatataaga atatacatat gtatcatgga tatagagaat ctgatataaa tagtattatt
     2101 gatgataatt ttgaccctta tagtggattc cctgcatata gaagttgcaa gtgtaagatt
     2161 gaaaaaatga atagataaat ggagagatat aatgaaaagt ttatttaaat ttaatagaga
     2221 attatgtaca gcatgtggag catgtttgct tgcatgtata gaccaaaatg ataataatat
     2281 agataatggt gagattttta gacatgtatt tacaatagag gaaggtgaaa gtatagaata
     2341 tttttcatta gcatgtatcc attgtaatga gccaaaatgt ttagatgtat gtcctaaaaa
     2401 ttgttttaaa aaagaagaag gatttgttgt attagacaat caaaactgta tagggtgtaa
     2461 actttgtgaa aaagcttgtg aatatggagc cttaatttat ggaaatgatg gtaaggcaaa
     2521 taagtgtaat ggttgtgttg agagatataa gtatggtatg gattcccctt gtgtaatggt
     2581 atgtcctaca ggagcattaa aatttaataa ataaattaaa tatagagagt gacttatact
     2641 tgatataaga tatttttaca aaaagctata ttgagtttgt tataataaaa actcaatata
     2701 gcttttttat ggatatagtt ggatactata atgtgattct tagtaatagt tttttgctat
     2761 tggacaaaga ctgtaaaaca tattaaactt atgttgttca aagtcgaagt ataatataat
     2821 aactagaaaa ttgatttata gtgttataaa agcaaattta gtaaggtata aaatcagagg
     2881 caatccttta ctacagtatc aatatttggg agttatgtta tggcagagcc attaaacaat
     2941 tataatggta caagtgattc aacctttgaa aatgtattat ataaaaaatt aaaataagat
     3001 taaaaaggag agaataaatt atgaaaaaac ttaaattaat tattatgctt gccttggtat
     3061 cagcactttt aattacagga tgttcatcag gaaataataa aaataaagaa gaacaaaaaa
     3121 ctataagagt tgcaacatca ggaacatatt acccttttgc ttttaaagat aaagataaac
     3181 ttaaaggatt tgaagtagat ttttgggatg aatttgctaa acgaactaat tgtaaagttg
     3241 aatgggtatt aactgatttt agtggattat ttggattatt agaagctgat aaagcagatg
     3301 ttgtatctgc tcaacttact gcaacacctg aaagagagaa aaaatatgct ttttcagatg
     3361 cttataatta tagtggaaca aacattattg ttagagaaga tgatactagt ataaaatcaa
     3421 tagatgattt agttggcaag aaagtaggcg tgggtactgg agcagtagca aatgaaatac
     3481 taaaagaaaa gtacccaaat gatgaaataa aaatagtaaa ttattcaagt gcaactctta
     3541 aagggaatta tcaagatcta gaaatgggaa gattagatgc agtagttgcg caggatgttg
     3601 aggctttaat agctataaaa gaacaaaaat taaatcttaa aatggtagaa cctccaattc
     3661 aattcggagc ctgttctttt gcgcttcaaa aaaatgaaaa aggtgaaaaa tggacaaaag
     3721 aaattaacga agtaattgaa gagatgcata aagatgggac tttgactaaa atatctgaag
     3781 cctggctagg aaaagacatt actaaggagc ctattaatga gtaattttaa ttttgaattt
     3841 tgtctaagta tatttccatt tttgattaaa gcattgggaa taacaatact aatatctatt
     3901 gttgcatttt ttttatcaat attattaggt gtaatacttg cctttttcac acagacaaaa
     3961 tcaaaagttt tgcaatacat aacaagagtg tatgtttcat tctttagagg aacaccactt
     4021 ttaattcaaa tatttttctt ttactttgga tttccaatga tattttcttt tatgaaagat
     4081 tgttcacctt atttagcaat agtaatttgt ttaggtgtaa attcatctgc atatatggct
     4141 gaaattatac gagcatctat tgagagtgtt gataaaggac aacaggaagc atgttatgct
     4201 tttgggttaa gtaaatatca gtcaatggtt aaaataattc tacctcaagc agctacatct
     4261 gcagtacctc ctattgtaag ctgtacactg gacataatta aaggttcttc tttaggtcta
     4321 actataggaa tacaagattt aatgggagta gctcaagtag aggctgcttc aagttttaaa
     4381 tattttgaga tatatatgat aacgatatta atttactggg cagtatctat aatattgagt
     4441 aatatacaaa gcaaagttga aaaaagaata ggaaaagcat attagatttc aatatttaaa
     4501 aatgggatga tgtagatata ttattacttg taaataaatt tttaatttta gaaaaataaa
     4561 cttagtaatc taaattattg agaaaatata gtatcaaaat ggactgtata atcaagttga
     4621 aaaatagttt ttctattgaa aaaataaata aaattctatt gtaatttaat atgcgacaaa
     4681 gttttataaa aaacaacatt aagctagggg gacttatttt atgattttta atatgaaata
     4741 ttttttagaa ttatttccaa taataatgaa atcgttcaat gtaactgtaa ttttggcaat
     4801 aagttcatta gtattttcat taattatagg tacaatagta gcattatcat cttattacaa
     4861 agtgaaagta ttgaatcaaa tatgtaaagt atatgtatct atctttagag gtacgcctct
     4921 tatgccacag ttattctttt tatattttgg actagcgtat atgaacgatt ttgttaaaaa
     4981 tatggaccca gtatttgcaa catctatagt acttagttta aatatgggag cttatatgtc
     5041 tgaaacgata agggcatcta tactttcaat tgataaaggt cagatagaag cagcatatgc
     5101 aatgggatta acaaatttac aaacaatgaa aaggatagtg ataccacagg ccgtaagagt
     5161 tgcactgcca tcattattca ataattttat agatttaatt aaagggtctt ctgtagcttt
     5221 tgttgttgga gtaaatgata taatgggtgc tgccaaatca caaggagcgt tgagttttaa
     5281 gttctttgaa gtgtatggag cagttatggt aatttactgg tgtataataa ctcctttagg
     5341 gtgggggcaa aaaaaattag aaaagaaaat aagcaattat taagttatta agaaaataca
     5401 taaaagtatt tttatataaa aaatggtgtg aggagaagag atatgataaa aagaaatatt
     5461 ataaaatgtt tagtggcagt tttatcgtta acatttgtag ttgctggttg ttcaaaaaaa
     5521 gaggaagtaa aggatgaaaa agttataact gtagcttcat ctaattcagc tgacccatat
     5581 tcttatgttg ataatggtga gcataaaggt tttgaagttg acatgtggaa agagattggt
     5641 aaaagaagtg gatataagat aaaaatggaa ccaacaggtt ttagttctat atttgggatg
     5701 ttagattctg gtaaagttga tgtggcagcc aacttctttg gaatgtctaa agaaagatta
     5761 gaaaaatacg atgcttctat accttatggt tctgattctg tatgtatagc tgtaaaagat
     5821 ggaaacaacg aaattaataa acttgaagat ttaaaaggaa aagttgtagc agtttcagaa
     5881 ggttctcaag ggcaacaggt tgcagctagt gttaataaag ataatttgtt tgaagaaaag
     5941 gtttatggtg atggaactaa tggtcttcaa gatttagatt taggaagagt tagcgcatgg
     6001 attgatgcag aacttactgt tgtaggagac gctaaaaaag ctaatatgaa ggtaagagta
     6061 ttagatgaaa aattatcagt tactaatata gcatacttct ttaagaaaaa tgatgaaaaa
     6121 tctaaaacta taaaagaaga tgtaaataaa gcaatagaag aaatgcttgc agatggaaca
     6181 gtgaaaaaaa tatctgaaaa atggtttgga atggatgtaa gtgctgatat acaaaaataa
     6241 atactttaaa attaaaatat aaaactgtga taactataga taagtatact aaatttatct
     6301 atagttatct gtgtattaca atgtatttag attagtcttt ataaattaaa atgagttatg
     6361 gaggtcttgc aaatgattga aactaaaata aaatccattg cactaaaaaa tagagaaaaa
     6421 atcataaaaa taagacgaca gatacacagt aatccagagt tagcatttaa agaatttaaa
     6481 acatccaaac ttataaaaga agagctaaat aaacttaaaa tagaatacat agatgttgct
     6541 ggtacaggag tattagctac tataaaagga aaaaataatg gtgggaaaac tatattactt
     6601 agagcggata tggatgccct tccaatcaaa gaggaaaatg atttagaatt taaatcaata
     6661 aatgataata tgcatgcttg tgggcatgat gcacatgttt catggttgtt aggaacagca
     6721 atgattttaa atgacataaa agaagaattg aatggaaatg taaaattact atttcaacct
     6781 ggcgaagaaa aaggtggttc tgatattgta attaaagaaa atgtacttga aggtgtggat
     6841 gcattagcaa ctggtcattg ttggccaact attgaatcag gaaaaattgg aatcgctaga
     6901 aattgtgcta tggcagcgac taatactttt gaaataacaa taattggcaa gggcgggcat
     6961 ggagcagagc ctcataattg tatagaccca attgcagttg gaaatgcagt atattcatcc
     7021 attcaacaga tagtttcaag aaaaattgac ccagttgtac ctgtagtagt gtctgtttgt
     7081 tcttttaatt ctggtgtttc taaaaatata ataccagatg tatgcacact tcaagggact
     7141 attagagcga taagcaagga acaagtaatt gaagtaagta aaattctaga aaatatagta
     7201 agaaatgtat gtagagcata tggagcagat tgcaaatttg aaaagagtac aggaggagat
     7261 gctgttataa atgacaaaaa catgatagag ctaggaagag aaagtgcttg caaaatactt
     7321 ggagttgaaa atgttgaaat aattgatttt cctgcaatga caggtgagga ttttgctatt
     7381 tatatgaaag aaaaacctgg tttatttatg tatataggtg taggaaataa agaaaaaaat
     7441 ataaactaca gattgcatag taacaagttt aatatagatg aaaaatgtct tagtatagca
     7501 tcttcattat tctcacaact tgcagtagat tatttatgtc aactatagag tacattcata
     7561 aaaatatatt tagcctattt ttaaatttaa tgctagtata ttaattttga tgtagaacat
     7621 attatttatt atatttattt aaaaaggtgg ataacaatga taaaagtaga aaatttacat
     7681 aaaagtttta aagggacaga agttcttaaa ggtataaatt tagagataaa tcaaagtgaa
     7741 gtagtagcta tacttggacc ctctggaaca ggtaaatcta cgttacttag atgtttaaat
     7801 tatttagttg agcctacaaa aggaaagata atgattggtg atgtaaaagt agatgtagaa
     7861 aaagcaacaa aaaaagatat agccaattta agaaaacata catctatggt atttcaaaat
     7921 tataacttat ttaaaaataa aacagcatta gaaaatgtaa tggaggcatt gatagtagta
     7981 cataaaaaga gtaataaaga agctagagaa atatctttaa aattaataga taaagtaggt
     8041 atgttggata ggaaagattt ttatccatca aaattgtctg gggggcagca acaaaggata
     8101 ggtatagcaa gagcgatggc agtaaatcca aatgtaatat tatttgatga gccaacatct
     8161 gcacttgacc cagaacttgt tggagaagtt ttaagcgtta taaaatcgct tgcaaaagaa
     8221 ggaataacaa tgcttattgt aactcacgaa ataaattttg caaaagaagt tgctgataaa
     8281 attatattta tggataatgg tgtaatagct gaagaaggaa ctcctgatga aatatttaat
     8341 aatcctaaaa atgaaagaac tgcaaaattt ttaaatatcg ttaggaaaga agaagtattt
     8401 agttaaaagt gtataaatta agtattagaa gatatgtaag atactcccat taaatttata
     8461 agttagataa taaatttaat gggagtatct ttttattatt agatttatta tttaatttaa
     8521 aattagaaat gtaactattt gaaaattctt tttaaaagta tatcaaaaat atcaagtaca
     8581 tgaaaaaata atatttgaca aaatatcata tatgtttaca aaattaactt gatggaatga
     8641 taaagcatag gaattacaat gagtatacta aaactttata ttaaagtaca aattattaaa
     8701 tttgaaaata attaagtaat attatatata ttttttatat tgtttgtttt attaaaatta
     8761 atctagaaaa aaagttttaa aagcagaatt ttattaatat ttatatatag atatgaacta
     8821 ttaataaaat gttaaagata caaaaaaact atagaaattg acatatattc cagtatttgc
     8881 taaagtagct gttgggacaa attacaaaaa ttattaatgc gcattagata atttacttta
     8941 atttttttag gatttagggt aatttataat ataaaacatt agaaaggaaa atataaatga
     9001 gggacttttt aaaaaattat aagtcatcaa ttatactttt agcatgtact ataatagggg
     9061 cgatagtagg attagtactt ggtccaaaag cttcaatact aaaaccattt ggagatttat
     9121 tcataaatgc tctattaatg atagtagtac cattagtttt tcttagtgtt tcttcaggtg
     9181 ttgctagtat aaaaaatatg gaacgttttg gaaaaataat gaagagtaca gctatagtat
     9241 ttatgggaac aacacttgta attgcatttt taggtattac atctgcatta ttatttaatc
     9301 cgtctaaagg attagatgta tcaagtgtaa aaagtttaat ggtggctcaa gatgcagaaa
     9361 ctttagaatc aaaaggaacg ttattagacc aagtagtatc aagtattaca gttaaaaatt
     9421 ttggagattt attttccagt agcaatttac tacaattagt tatatttgca gttttagttg
     9481 gaacagcaat agctatgatg ggagataggg gaaatacaat aagaaagctt ttatcagaag
     9541 gaacggaagc aatattaaaa gtagtaagta tagtcatgct ttatgctcct atagggctag
     9601 gctgttatac agctacaata attggggaac ttggaagcaa aataattcaa ggatatgtaa
     9661 gggtgtttgt aatttattca ctaacagcag ccatgtattt cttattattt tacagtctgt
     9721 atgctttttt atctgcagga aaacaagggt ttagacaatt ctggagaaat gctataaaac
     9781 caatagcaac ggcactagct acttcttcaa gttcagcatg tataccatca aatttagaag
     9841 ctagtaaaaa tataggagtt cctgatgata tatcagaaac agttatacct cttggaataa
     9901 atatacataa agatgggtca gttttagcag taataataaa agtggcattt ttatcaggta
     9961 ttttaggcag tggaatggca aatccagcaa cgataggtca attatttata actgtatttg
    10021 ttgttggagt atttatggct cctgtagcag gtggctgact tattggtgag atgctcgtgg
    10081 ttagcatctt cggttttcca gtggaggcaa ttccattgtt aacagttata actactataa
    10141 ctgatattcc taatactata ttaaatacaa ctggaaatac agtatcatca atgttagttt
    10201 caagattagt agaaggaaaa gattggctta tagataaaac agcaattact acaaagaaaa
    10261 taagctaatt gtacacttaa ttaataaagt taataggtat cttaatttta ataaattaga
    10321 tatctattaa ctttttaagt aaaaatataa atttaagatt caaatatgat atttataatt
    10381 tgaattaact aatatagtat atattgaaat aaatatgttt aaatatctag tttatttttg
    10441 aaagcaaaag atttcaaatt tatttttatt aaaagataac aagccttcat cccgcatctt
    10501 gccaagctct cttgacatag aacttctgtc aacacataaa aaatcagcca tttcatttct
    10561 tgaaaaaggg attgtaaact tgtggctacc tgtttgttct atctgctttg aaaaatacat
    10621 aagcaatttc tcgcgagttg ttttacgaga aagtaattct attttattgt gaagtaaaat
    10681 gtttttttca gctattaatt gtagcatatt ttcaacaagc ctattatgaa atacacatgt
    10741 agatgaacaa ataccagtta cacttttaaa ctttataaat aaaacttcgc aagaagatgt
    10801 tgtggttact gtaacattac ttttatgtag tctagtacat gcgaatacct ctccaaataa
    10861 atctccaggc aagagttcag ctacaatatt cctattccct tcaatatctt cctttattat
    10921 atgagcactt cctttaagca taattccaat agaagtaact tcacttccaa cagagaatat
    10981 tatactatct tttgggtaag ttttatctac aacacctaaa cattccaaca tttcttcaag
    11041 ttcattactt ttaattcctt caaatagagg aatttttttt agtagatcta tatgcttttc
    11101 taaaaatttt ttcaaataat cacttccttg ttgcatatgc aaccgataat ttgttttcat
    11161 tgtactatac taagtttata aaagcaaggg gatataaaaa tcctactaca agaaatttga
    11221 aataaaaaaa tataagtatt aaaaataaat atataagaaa tcaatacttt aaattaaaaa
    11281 aattttatga ggtgatataa atgatgagaa aaataattaa aattgatgag ggaaagtgta
    11341 atggttgcgg actatgtgtt gaagcttgtc atgaagaagc tataggaatg gtaaatggaa
    11401 aagcaaaact tttaagagat gactattgtg atgggctggg agattgtctg ccaacttgtc
    11461 caacaaatgc gatttcattt gagtatagag aagcagcaga atatgacgaa gctgcagtaa
    11521 aagcaaatat ggaagcaaag aaagcacaaa agaaaacttt agcatgtgga tgtccaggtt
    11581 ctcagtctaa gtcaataaat agagaggttt caaatagtac atctataagc aatgatatag
    11641 tagagacaaa aggttctcaa cttaatcaat ggccagtaca aataaaatta gtaccaacta
    11701 atgcatcata tttaaagaat gcaagtcttt taatagcagc agactgtaca gcgtatgcat
    11761 atggaaattt tcataataaa tttatgaaaa ataaggttac attaatcgga tgccctaaac
    11821 ttgatgaagt tgactatgct gaaaaactta cagagatttt aaaagaaaat gatattaaaa
    11881 atatagtagt aacacgtatg gaagttcctt gctgtggtgg tatagtaaat gctgttaaga
    11941 cagctctaca aaatagtgga aaaatggtac catggcaaat agttactata tctgtagatg
    12001 gaaaaattgt ggatggagaa atttaattaa gtgaattata gaaaaatcta ataaatttta
    12061 ataaataaaa agggaggaat aaaaaatgga aaaaatgttt tgttttcaat gtgaacaaac
    12121 agcaggaggt aaaggttgta ctataaatgg tgtttgtggt aaaaaggggg ggacagctaa
    12181 cttacaagac gaacttacag gagtgttgat tggtctttca agagcaacta ttggaaataa
    12241 gaatagacca acaagtgaaa cagataaaat tatgatagag gggctattca ctactataac
    12301 gaatgtaagt tttgatgatg aagctataaa aagacaaatt gaaaaaactg agatagaaaa
    12361 aagtaaactt gtgcctagat gttcagactg ttcaacaact tgtaatagaa atgataacta
    12421 tgatatgaag gatttatgga atgataatga ggacatacgt tcattaaaat cattaatctt
    12481 atttggatta agaggtatgg ctgcctatgc atatcatgca atggtacttg gttatacaga
    12541 caaagacgta aatgagtttt tctatgaagg attaatagca gttggaggag acttgtctat
    12601 tgacaaactt ttaggtatag ttatgaaaac tggagaaata aacttaaaat gtatggaatt
    12661 gcttgataga gcaaatactg aaacttatgg aacaccagaa ccgactcagg tatctatgaa
    12721 aatcgagaaa ggaccattta ttgtagttac aggacatgac ctttatgatt tacacaaatt
    12781 attagaacaa acaaaagata agggtataaa tatatatact catggtgagt tattaccagc
    12841 acatgcatat ccaaaactaa aagaatataa acatctaaaa ggtaattttg gtacagcatg
    12901 gcaaaatcaa caaaaagaat ttgataatat tcctgctcca atattattta caacaaactg
    12961 tttaatgcct gttaaagata gttatagaga tagagtattt actacagaag tagttgctta
    13021 tccaggtatg gttcatattg atgaagataa agatttttct agtgtaatag aaaaagctat
    13081 tgaacttgga ggatataaag aagatgttca tatgactggt attaatggag gagatacatt
    13141 aactactggt tttgcaagag gtacagtttc atctgtacaa gataaagtag tagatgctgt
    13201 aaagagtgga gctataagac actttttatt ggttggaggt tgtgatggtg caaaacctgg
    13261 aagaaattat tacactgaat ttgtaaaaca agctcctaaa gacactgtta tattgacact
    13321 tgcatgtgga aaatatagat ttaatgattt agaccttggg gatataggtg gcttacctcg
    13381 tttaatggat atgggacaat gtaatgatgc ttatagtgca attaaagtgg cagtttcatt
    13441 ggcaaatgct tttgaatgtg gagtaaatga tttgcctctt agcatggtac tgtcatggta
    13501 tgagcagaaa gctgtttgta tacttcttac actacttcat ttaggtatca aaaatatcta
    13561 tatagggcct tctttgccag cattcttatc taagaatgta ttagatatat tggttgataa
    13621 atttgcattg acacctatat ctactcctga agaagatatg aagaagattt taggataaat
    13681 aaatatatct atacttaaat atatttatag ttaaaatatt tttaagtatg acacaattaa
    13741 gataaggggt taaacttaaa atttgattga aaaaatcaat tttgagacga ccctttattt
    13801 attttttgta ataattgaaa aataaacctg ctttcagtat attcttttta agaatctttg
    13861 tattaaattt ataatactta gatatataat atcttatata ttttatttta agtattaaag
    13921 gtttgaatgg aacttaaata ttaaaagagg aacattgtaa tatgtaactt gcataaaata
    13981 tatatactat agtgaaatta ttttgattaa aatttaaaat ataaggtgtt ttatgtgggg
    14041 ggtatatgta tatgaaaaat tctaatttta atatattggc atttgatggt ggtggattaa
    14101 aaggggcatt gagtatatcc atacttgaga ggatagtaaa agagtatcca aatctattaa
    14161 acaatataaa tatgtttgga ggtacatcta cagggagtct aattgcacta ggacttgcat
    14221 atggtgtaag ccctaaagag ataaaagaac tttactcaat tgaaaattca aagtatattt
    14281 ttaataaaag ttatgcagaa atactcagac caaagtatga aaacaaaaac ttaaaagaag
    14341 ttttattaag tatatttcca gaggaactag aattaaaaga tttaaataaa ttagtaatga
    14401 taccaagttt ttatatagga aatgaggaaa atgcatggaa accagtgttt tataacaata
    14461 tgacaaattc ttttacaaag actagcaaag ttgtagatgt agcaatggca agtagtgctg
    14521 ctccggtatt ttttccaaca tataatcgcc atgttgatgg aggaataata gcaactgacc
    14581 ccagtttagc gtgtataatt catgccattg acagtggatt taaacttaag aatacaagac
    14641 ttttttcaat agggactgga tatgtatata atagtataaa ggcagatact actgaatggg
    14701 gagctattga ctggataatt aataaagaac ccgatttacc aattatatct attacattag
    14761 aaggaaattc tcaaatgagt cagttatttt ctcaaaaact tcttggagat aattactata
    14821 gattaaatcc taagatggaa aaagatgtag ctatggatga ttgtgatgca ttagaatatt
    14881 taatatcttt aggagaaaat tgtgatataa aagatgcttt tggatggata aagaaaaaga
    14941 gcttttatag aaattagatt caatttgcaa ttgtattttt acacataatt tcattgagta
    15001 ggtaaaaata ataaaatgca gattattaag ttacttctta gggaataaat aaagtataat
    15061 ttatttaagg agtgatgaaa atgataaaac ttgctacttt tgcaggtgga tgtttttggt
    15121 gtatggtaaa accttttgat aagtatgatg gagttattaa agtaatttct ggatatacag
    15181 gtggtcatgt tgaaaatcct acatatcaag aggtatgttc aggaactact ggtcatttag
    15241 aagcaattca aataacatat gatgatgata taataagtta tgatgaactt ctaaatatct
    15301 tctggaaaca gattaatcct acagatgatg gtggacaatt tggtgatagg ggaactcaat
    15361 ataagactgc tatattttat catgatgaag aacaaaaaaa tatagcttta gagtcaaaag
    15421 aacatctgga aaatagtaaa atatttgata gtaaaatagt gactgatata attccagcaa
    15481 ctaaattcta cgaagcagaa gaaaatcatc aggattatta taaaaaaaga ccattaaact
    15541 acaatatgta ctataaaggg tctggtagat ataattttgt aaaaagtcat tgggatggaa
    15601 acaatataaa tagggaagag ttaaaaaaga ctttaactcc tatacaattt gaagtcactc
    15661 aaaatgatat gacagaggtg ccttttgaaa atgaatatta tgataatgac aaagaaggaa
    15721 tttatgttga tattgtaagt ggagaagtgt tattttcttc aaaagataaa tttgattctg
    15781 gatgtgggtg gccaagtttt tcaaaaccag ttatagataa atctatcatt gagagaagtg
    15841 atttctcaca aggtatgtac agagttgagg taagaagtgc taaaggaaat tctcatttag
    15901 gtcatgtatt tgacgatggt ccaaaagagc ttggaggttt aagatattgt ataaattctg
    15961 cttctcttaa atttataccg agagaaaata tggaagcgtt aggatatgga gaatatttaa
    16021 aactttttga gtaattagga atatttatta taaacttagc taaaaggtga aaatataaga
    16081 aaatattttc accttttgtt tttttgtcaa taatatgata aattattaat tgtaatctta
    16141 tttataccaa tagattaaga atttaatttt gaaagagaga aaacaaatga atttaaaaga
    16201 ctttaatgga aagagattaa aatcagctag aatttttaga gcaaagacga tagaacagtt
    16261 gtctaaagaa actaaaataa ataaaaaaga tttaaaagct tttgaggaaa ataagtatgt
    16321 accaaatata gaaaatacat taaaattatt taatatatta aattttccaa aagagtattt
    16381 ttataaaaat gaaaatataa atatcgttgt agaggatagc cactttaacc ctcaatctaa
    16441 actacctaga gtagaagaga tttcttatag agaaaaaata attataatac ataaaatata
    16501 ttcatttatg gagaattata ttaagtttcc aaaagaaaac ttgccaaata gaaaagtaat
    16561 gtcagatatt aatataaatg atatagaatc tcttgcatat aagactagag agttttggga
    16621 tttaaatagt actccaatag taaatatggt aaatttgtta gagtctaaag ggattataat
    16681 atcaggtatg aatgtagata gaaaaggggc aactatcttt actcaaaaac agagaattag
    16741 taaagaatct aaatatctaa tatctctcgg taatgacaaa aagtcagcat caattagaaa
    16801 ttatactttg gcatgtgagc ttggatatat aatatcaagt gaattaagaa taccaagtaa
    16861 gcaattttca gaagatgagt atgcttgtgc atttttattg ccaaaagaga gttttttaaa
    16921 agatttgact catccagaag atttagatta ttatgtagaa ctaaagaaaa aatggattgt
    16981 acctatatct gctatgattt ttagagcata taatctagaa aagataaatt acaaaaaata
    17041 caattatctt atgaatgaaa tggataaaaa aggctggttg atagaagaac cactagacaa
    17101 aatgaaaggt tcaagcccaa cgtatttaaa aagagcagtt gaattattga tagaaaataa
    17161 aataatgagt gtaaatagta tagtatcaaa tttagaggaa tttggaataa atctttatcc
    17221 agaagactta gaattattga tgggattgaa aaaaggattg ttatctcaag aggtaaataa
    17281 aaaaagtaaa gttataaaat ttgatggtaa aaaatagatt tatattttag tattaaaatt
    17341 aaaagcaggt ataaatgatt taaacattaa cttagttaaa agttttttca ataagtcatt
    17401 tacctgcttt tagtgtaaaa aagaatcttg aatctaaagt gtttttgaaa gtgcaatata
    17461 ttctcttaaa acagagcaag atttattaaa ttcgttttgc tcatattcag tcatattgat
    17521 ttcaataatt tcttcaatac catcttttcc tataacacat ggaacacctg caaatacatt
    17581 tctttcgtta tattgacctt ctagtaatgt agaaactgga agaacttttt tctcattgtg
    17641 tagtatagct tttacaattt ctactaaagc agtacctata ccaaattctg tacaatcttt
    17701 accatttatt atacttatcc ctgttttttt atttttttca accattgatg ataggtctaa
    17761 attgctataa gtatttgggt tgtcttttat catgtctaag attggtttac caccaattga
    17821 aacatgagac caaggaacca tttgagaatc tccatgttca cccatagaat aagcttgaat
    17881 tgattgttga gcgattccag tttcttcaga taaaatcctt ctaagtcttg ttgaatctaa
    17941 agctgtgcca gttccaatga ctttgttttt aggaaagcca gatttttccc aaatataatt
    18001 tgtaactaca tcaacaggat tagatatgac tacaaatata ccatcaaagc cagaatcaac
    18061 tatgggtttt actatagatt ttatcattcc aatagttggt cttagtaaat ctaatctagt
    18121 tgtgttttgt ctacgtaaag gtccttctga agagtctgca acacttatca ctactatgtc
    18181 agaatcttta cagtcagaaa aagttccttt ttctatatgt actttgtgtg gcagatatga
    18241 aactgcatca gctaaatcta aagcttgagc ttttgattta gattcatcta tatcaatcat
    18301 aaagagttca tcacaaactc cttgagttat taaactgaat ccacaatgag aaccaacatg
    18361 ccctgaacct acaatagata tttttcttgg ctttattgcc attttaaatc actcccccta
    18421 aaataacgat acatttataa gaaaattata gatgttatta aaagctgttt ttgtatattt
    18481 taatattcta ttatttcaga aaatttattt aatatgattt tatactaaaa tttttaaaaa
    18541 agcaagg
//
LOCUS       DAEORZ010000080        18437 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEORZ010000080 DAEORZ010000000
VERSION     DAEORZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.080"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            368..1411
                     /locus_tag="KRH39_002375"
     CDS             368..1411
                     /locus_tag="KRH39_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512274.1"
                     /translation="MLKKWFGIVKKRQKSESAKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCEKEVQ
                     VESEKIGLNKFEGLYQNENYNLEKNVIEEKEVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            1522..2664
                     /locus_tag="KRH39_002376"
     CDS             1522..2664
                     /locus_tag="KRH39_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG8512275.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
     gene            3118..4509
                     /locus_tag="KRH39_002377"
     CDS             3118..4509
                     /locus_tag="KRH39_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG8512276.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            4762..6555
                     /locus_tag="KRH39_002378"
     CDS             4762..6555
                     /locus_tag="KRH39_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG8512277.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(6636..8018)
                     /locus_tag="KRH39_002379"
     CDS             complement(6636..8018)
                     /locus_tag="KRH39_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG8512278.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            8276..8623
                     /locus_tag="KRH39_002380"
     CDS             8276..8623
                     /locus_tag="KRH39_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG8512279.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            8641..9675
                     /locus_tag="KRH39_002381"
     CDS             8641..9675
                     /locus_tag="KRH39_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512280.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(9724..10773)
                     /locus_tag="KRH39_002382"
     CDS             complement(9724..10773)
                     /locus_tag="KRH39_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8512281.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            11048..12583
                     /locus_tag="KRH39_002383"
     CDS             11048..12583
                     /locus_tag="KRH39_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512282.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(12769..13455)
                     /locus_tag="KRH39_002384"
     CDS             complement(12769..13455)
                     /locus_tag="KRH39_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBG8512283.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALNSI"
     gene            13584..14966
                     /locus_tag="KRH39_002385"
     CDS             13584..14966
                     /locus_tag="KRH39_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG8512284.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            15368..16636
                     /gene="eam"
                     /locus_tag="KRH39_002386"
     CDS             15368..16636
                     /gene="eam"
                     /locus_tag="KRH39_002386"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBG8512285.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(16729..17085)
                     /locus_tag="KRH39_002387"
     CDS             complement(16729..17085)
                     /locus_tag="KRH39_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512286.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(17236..18330)
                     /locus_tag="KRH39_002388"
     CDS             complement(17236..18330)
                     /locus_tag="KRH39_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG8512287.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTTALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         7340 a         2153 c         2993 g         5951 t
ORIGIN      
        1 tatgtaaata attaatatat aataccatta tgggcaataa atacaaatca aatgataata
       61 tagtctattt atgtaaatgt tatattgtat cctaagtata atagtaagtt ttttgatagt
      121 ggtgtagaaa gtaaaaatct actagcctaa attgatgtat ggattagttt aggaacagta
      181 attgcatatc ttaagtttga tgtcatgttg ggctactaga aataggtcat ctttttcact
      241 tgaatatctt ttctttaaac atggagagta tcatatcaaa aacatatgaa aattaagtga
      301 tttataagaa cagtaatatt ttatatgata gtatagaata gtaataaagt attaggagga
      361 acattgagtg ttaaaaaagt ggtttggtat tgtgaaaaaa agacaaaaaa gtgagtcagc
      421 aaaagaagaa ggtgaagtaa tattaaaaaa cgaaaaaata ttatctgaag aaaagttgat
      481 agatgaagaa ggagttgtag ttaatattga taatgaaata ttaactaaag tagaagtagt
      541 aaatgatgat aatgaaatag aagaaaaaat aatagaagaa gattggttaa ttagtgaaaa
      601 taccataaaa ttagatgata aagaagcaat tattaatgat aaaaacatag aattatgtga
      661 aaaagaagtt caagttgaaa gtgaaaaaat aggtttaaat aagtttgaag gactttacca
      721 aaatgaaaat tataatctag aaaaaaatgt tattgaagaa aaagaagtaa gtgaatgttt
      781 gacagaagaa gatttagagt acataaaaga aattaaaata aaaagaggaa aaagtataaa
      841 agctataaac ttgtatacta aagaagagtg ggtttttgac actcatatac agtgtagtaa
      901 aaaactcaaa gttccattag ggtacataag agaaaattta aaatatggat atatggatta
      961 ctttggagat gcaataaatt atttaagtga agtattaaat atagatgaat actgcaaaag
     1021 tgaatggagc tatctcgata atagcaaatc tccatctgag atatttaata ttctaaacaa
     1081 taaaatattt agtataaggc tttctaatga aaaaagaaat gaaattttga caaatgataa
     1141 aattgaagca ttaaaaatga attatagatt tgaatgtatt gatgaagaat atgatgaata
     1201 ttttaaaaaa tataagtcta taatcaaaag aggtggaaag aaaaaagttg aattagtaaa
     1261 taaaaaaggt gacattttag aaatatttaa gtctttagaa gaatgtgcca tttatttgca
     1321 aaaggaaaag aatgaagtta tacaaatgtt aaaatatgga gatacaaaag taggaagaaa
     1381 ctttataagg tatagtttga gaagtattta atttcatttt aaattcataa gagtttcatt
     1441 tctgattcac acatttaata ttattttaat gtaaaataat aaaggtgaag tattgcacaa
     1501 ttaaaattaa ggagggtatt aatgtctgaa gaaaaaaatc caaaagaaaa tacattagat
     1561 gaatctaaaa attcagataa tgtaaattct aaaaaagatg atgtgtcaga tgaaaaaaat
     1621 actgataaca aagttaaaaa aagggaaata aagctaaaca acaaaacact taaagctatt
     1681 gtagcaattg ttgcagtagg agctatatgt ttaggtggag gctatgtttt tggcaaagaa
     1741 gctggaagaa aattgcctgc tacttcaaaa cactataata gcagtgaggt agtagctact
     1801 attggggata aaaaaataac aggtgaaatg ttaagaaaga gaatggagcc attattttat
     1861 ttaaatgcaa aaactactat gactgatgac gaaatacgtt catatgaatc aaatatgatt
     1921 gaaactatga ttacatctga tatattatcc tctgaagcag caaaagagaa aataagtata
     1981 actaatgacc aactagatgc tcagtataat aatttaatga gtagtataga atcagcaatg
     2041 ggaatgacta aagaagagtt tttaaaacag tttgatttga ctgaaaaata tataaaagat
     2101 gatatgaaaa aagagctagt agcatcaaaa tatttaaatg aaaaatcaaa agttactgat
     2161 aaagaagcag agaactatta caataaaaac aagagcaatt atttacaagt tagagcttca
     2221 catattttaa taaaaactgt tgatgataaa ggtaagcaag ttagtagtag caaaaaagct
     2281 gaacttaaga aagaagctga agaaatactt aaaaaagccc aagctgggga agattttgct
     2341 actctagcta aaaaatactc tgaagatagt agtgcagaat caggtggaga tttaggtttc
     2401 tttggtaaag gtcagatggt ggaatctttt gaaaaagcag cctttgcatt gaagaaggga
     2461 gaggtatcta acaaacttgt agaaagtgat tatggatatc atataattaa gaaaactgat
     2521 gaaaaatatc agccatttga agaggtaaaa tcagatttag taagcagttt aacatcagaa
     2581 aaacaaaacc tacttataca aaatctaaaa gaaaaatata atgttaaaat tacagataaa
     2641 tatcaaatga aagctaaaga atagataaag tcaatttatt aaaaaaaaga aaaattaact
     2701 gtatggataa aagctatttt tactctcaat atagagagta taaaatagct tttattattt
     2761 tgcaataaaa tctatttata tatatgaagt gataaagaaa taacatgcaa aaaggtatta
     2821 aaaagaaata tttttaattt gaattttttc aataaaaatt tacaaaaatg aaaaaataaa
     2881 caaaaaagaa ttgaagattt aagttgttat ttaaatatat atatattaag attttctgct
     2941 gaagatacta aaaaaataat atttttcata attttaaaaa taataataaa aaaatgaaaa
     3001 tacaggctat aatctattga gtaaacatag aaaagatgat attataagta tgtgctaaaa
     3061 tggttaaagc aatattaata ttaaatttaa ctataaaacc aaagggggcg tttttttatg
     3121 tcaagtacag aaagcgtatc taaaaaaagt ggtcatccga agagtttttg gcttatttgc
     3181 atgacaattt tgtgggaaag atttagttat tatggtttaa cagctttatt ggtattattt
     3241 tttacagcaa gtgtagctca aggtggttta ggtctttcta ctcaagaagc aacatctatg
     3301 tttggcttat ttacaggtct tatatatctt acaccattag tcggtggctg gttagcagat
     3361 aaagttttag gtcaacaaaa atgtatttct ataggtgcac ttttaatagc aataggagat
     3421 ttcgtactat ttgcttcatc tacatctatt gcaatggttt gggtaggtct atcaataatt
     3481 attttaggaa atggtttttt taaatcaagt tgctcaaata tagttgggga tatatatcct
     3541 aaaactgatg tgtcaagaaa agatgcagca tacagtttat tctatatggc agtaaatgta
     3601 ggagcattta tagctccaat aatatgtgga ttgctttctg ataatgtatt tgcagtaaga
     3661 ggagcaagtg gagatatagt atcttatgga tataagatgg catttttaat ttgtggtata
     3721 ggcatgatgt taggtatgat tatatttaca cttcttgcac caaaattatt aaatgaagtc
     3781 ggaaaatatc ctgtgactaa aggtaataaa ggtgaaaaaa ttgagtatgg accacttact
     3841 aagttggaga aaaataggat aatagcaatg gttattttat ttttatttgt ggtcttattc
     3901 tggacagcat actatcaaac acaaagttct tttatgattt atgtaaggga taatgtaaat
     3961 agaactctat ttggatttga aatgccagta gcatggctta catcactaaa tgctatatta
     4021 tgtgtggctt tatgcccagt attagctacc ctatgggtta aattatctca taccaaaaga
     4081 ggtgatttct ctattccagt taaaatggga ttaggaatga taataatggg tgttgggttt
     4141 atagttatgg tatttgctac attagctaat ggtccagatg gaatggtaaa ggctagtata
     4201 ttgtttatag tattgactta tatatttaca acagtaggtg aattattctt atctcctata
     4261 ggacttgcaa tgttcaataa attagctcca gctaagtatg ctacgttgtc tatgggagcc
     4321 tggtatctaa caagtttttt tgcaagttta ttatcaggaa agattgctgg ttttacagcc
     4381 acacttggtt ttttacagat atttggagga atagcaacag ttttaataat atttggttta
     4441 atattattgg gaataagaaa tgtattaaca agattaatgg ctatggattt actaacaaaa
     4501 gaaaaataat tcatgacgaa ttattgtaaa taaatgaatt actttaaata aaggaagtta
     4561 catttaaata taaaattaaa tgtaatttcc tctttgatta tgaaaaaaag ctttacacaa
     4621 atagatatta taattaaaat tataattgtg ataattagag tgtataagat ttatataaat
     4681 tatacaaaaa atatttatgt aataaacttt acatacttat atatttagtt attcaaacta
     4741 ttatttcgag gaggtcttgt tgtgaatata aaagatagat taagtggatt aagaaaattt
     4801 atggaagaaa aaaatataga tgcctatatg attccatctt ctgataatca ccaaagtgaa
     4861 tatgtaggag attacttcaa atcaagagaa tttatatcag ggtttaatgg ttcagcaggg
     4921 acggttattg tgactaaaga cgaagctggg ttatggacag atggaagata ttttatacag
     4981 gcagaatctc aattagaagg aagtacaata aaattattca aaatgggaca agaaggttgc
     5041 ccaactacag atgaatattt atataaaaat attcctgaag gaggaacttt aggatttgat
     5101 ggtagagtta tatctgctag agaaggagct acattagctg aaaaattatc taaaaaaggt
     5161 ataaagatag aatatcagta tgatttaata gatagtatat ggccagatag acctgcttta
     5221 tcagatagta aagcattttt attagatgtt aaatattgtg gagaaagttt tagctcaaaa
     5281 ttagctagat taagagaaaa aatgagtgaa aaaggtactt ctactcatgt aataactact
     5341 cttgatgata tagcatggtt attcaatata agaggtggag atgttaaata taatccagtt
     5401 gtactttctt atgcagtaat aactttaaaa gaagtttatc tatttgttga tgaaagtaag
     5461 ttaaatgaag aaatactaaa tgaactagct aaggaaaatg ttcaaataaa gccatataat
     5521 gatgtgtatg aatttgtaaa aaatatagac aaaactgaaa aagttttatt agatggaact
     5581 aagcttagtt atacaattta caataatata ccttgtgaag ttgagaaagt tgatgagttc
     5641 aatccagtaa tgttttttaa agctcaaaaa aatgaggtag aattagaaaa tataagaaat
     5701 tcacatgtaa aagatggtgt agctttcact aaatttatgt actggttaaa gaaaaatgta
     5761 ggtaagatgg aaatcactga aataagtgct actcaaaaat tagaggactt aagaagagag
     5821 caagaaggtt tctttgagcc aagtttcaat acaattgctg catataaaga gcatgctgca
     5881 atgatgcact atagtgcaac tccagagtca aattataaat tagaggcaga aggattattt
     5941 ttagtagatt caggtggaca atattatgat ggaactactg atataactag gactacagtt
     6001 ttaggaccta taagtgatga attaaaacta cactttactt ctgtagcaag aggaatgata
     6061 aatctttcta aggctaagtt cttgcatggt tgtagaggat ataacttaga catactatct
     6121 agaagttgca tgtggaatat gggaattgat tatcaatgtg gaactggaca tggtatagga
     6181 tttgtgctaa atgttcacga agctccaaat ggatttagat ggagagtagt acctgaaaga
     6241 tttgatagtg cagttcttga agaaggaatg gtaactacta atgaaccagg tatatatata
     6301 gaaggttctc atggtataag aacagagaat gaaattgtag ttagaaaagc tgaaaagaat
     6361 ttctatggtc aatttatgga atttgaagtt gttacgttag ctcctattga cttagatggt
     6421 atagtaccag aattaatgaa taaggatgaa aaagattatt taaactggta tcataagtta
     6481 gtttatgata aaatatcacc tttcttaact gatgaagaaa gagaatggtt aaaagtttac
     6541 actagagcta tatagtttgt actagataca ataaaaatcg aggtattata atgcctcgat
     6601 tttttattgt atattttatt aactttaaaa aatttttaat ttgataattc tactaatttt
     6661 ttattcatta taagtagtat aattccaaat atcaataaaa atgatggtat cactatgaag
     6721 acttgtaaca taccaagttg gtctataata gcttgcgtat agccagataa tttatttgct
     6781 gcaaatttgc ctaaaaacca aacacccatt aggaatgaaa tatacttttc aggcgaaagt
     6841 ttatttacca taccataccc gactggagaa aagcaaattt ctcctatact ttggaatgtt
     6901 aaaaatccaa ctacccaaaa tacacttgct ttgtgaattg aattagggtc tgttcctgtt
     6961 tgtataactg ctactatcat aaacaaaaat gctatggcta atagtataaa acctaagctc
     7021 attttttttg gcacacttaa atcgcctttt ttagtttgag ataattttac ccatactaaa
     7081 gcagagatag gtccaagaat aacacaaagg agaccattgt atgaatctat ccatgaagta
     7141 ggaatttgaa aattgccaac agtaagattt atgtgttcct ttgtatagag tgcaatagac
     7201 atagaggctt ggttatatgc tatccaaaaa aatactgtga aaaaagctaa caccaatatt
     7261 actattattc tttttttctc aatcaaagtt aaaggtcttg aagctagcat tttcttatat
     7321 tctagtgatt ttcttataga ctctctcccg ttactagggt cattattcaa aggtttataa
     7381 aactttctgt ttccaaatat aaagaagaat aaacctatta gaaccgtaat tgatgaaaat
     7441 aaaaatacat atctatatcc atacatactt atagataatc cgcttttcat agcaaaccat
     7501 tcattagcaa ctaaaccgca tattaaaggg ccaaataagg tacctagatt agtagccata
     7561 tacgtaatag agaaagctcc atcttttctt gggtcatcat tggtatataa gtttccaacc
     7621 atagtatgta agtttccttt ataaaaacct attcctaatc cagataaaaa tatcatgatg
     7681 tttatatcca tcttactatt tgctatgtat cctagaaaat aaccaattga aaccattagc
     7741 attccaagta taatacagtt tttgggacct aaaaatctat cactgaccca tccaccaata
     7801 actggagaga aataagtcca tgctaaaaaa gatgcaagca ttgatgcgga ttctgctggt
     7861 gttaacccta aaccaccttt ggagacttgt tcagctaaaa acaataataa taccaatttc
     7921 ataccatagt atgcaagtcc tacaaaggta aatattgaac agcagaaata taaatctcta
     7981 ggttgtttaa gtttttcttg cgtttttaaa actgacatat gtcgagaacc tccagataaa
     8041 tagtttaaca aaattttacc atactatatg agtatatgtt tttttcatat agacgtcaac
     8101 ttgtaagata atttaattaa gtcgaaatat atgtaaaaat agtatcattt ttgtgtattc
     8161 agtaatattt ttttatgata atatatgtta ttattatatt aaaatacaaa catatttatg
     8221 gaaaggatat gccaatttag tagttagatt gattgtttgg caggtgggat gagctatgat
     8281 aagaaagaaa tctgaattac aagtagaagt aaaagaaaat ttaaatggtg gtataggaaa
     8341 ggttaagctt gaaaacataa tacaaaatga agagcttaaa ggaaaaggaa gactatttaa
     8401 aagagtagct ttaccagtag gaagttcaat tggtgtgcat gaccatacaa cagattttga
     8461 agtatattat atactaaagg gaaaaggtaa agtatttgat aatggggaat ttgtagaagt
     8521 aaatgagggt gatgtagtgt atacagctga tggcgaaaaa cattctatag aaaatatagg
     8581 agaagaagat cttgagtttg tagcactagt tttatatatg taaaatatgg aggaggagtc
     8641 atgacaggca agatagaaga aattaaggac aagataaata gattttgcaa ctccaattta
     8701 aatcaagaat acaaagatat aagctttaaa attttgcaaa accttttaga taataataaa
     8761 gagataattc attcttctag agctgatata tggtcttcag ctatactaaa tatagtttta
     8821 gaacaaaatc ttttatacaa taagaagcat ccactacata taacaaaaaa agagttttca
     8881 aaacaaatag gtgtttcttt aaatactata aataataaat cgtctttaat aaaagatgtt
     8941 tgtagtattg actttttaaa tcaagatact atagctattt ctaatgtaga attttgggta
     9001 aaagagacca attccaaatc aaagtctgta gatttagatt tagaaaagaa aaaaatacta
     9061 tataaaagat atattaagct atcacaagat gcgaatagtg atattgaatc aattagatat
     9121 atggaggaag ctgtaaatat aggtaaaagt atgattacta aagaagataa agaacttgga
     9181 ttttggaaag gtttatctac cagagcctat atgatagcac ttgaatccct tgctagaaag
     9241 ttagaaagta ttaataatct aaaggagtcc aaaaaagtat tagaatattt aatagaaatt
     9301 aatcctagtg atgagcaggg aataagatac aaattattaa atgtattaat tagattaaat
     9361 gatagaactt caataaataa attatttgaa ctttataaag aagaaaaatc tgctacatgg
     9421 atgtacagta aagctcttta ttactttaaa aataaaagta tgtttttggc aaatgattct
     9481 attaaggttg ctagaagtaa aaataagtac gttggattat atttgttaga ttggcgaaat
     9541 gcttttggta gagaatttgt tacagaggaa gaaaaggcag aagctgtcta ttactatgat
     9601 gaaaacatag tgatttggaa tgaagttaaa ggttctatgg attggctatt aaaaaaaatg
     9661 ttggaatttt cataaaaaaa actactcctt acttttatat aagtaaaagg agtagttttt
     9721 aatttataca tctagtttta aatcttcagg ttttattaga cctttttttc tcataaattc
     9781 tgaaatcaac aaagttacaa tagcaggaag tataaaatta agtaaaagta taccaaccca
     9841 catagatata cctccttttc cagcactttg catagcagtt actgttccaa attgtccaac
     9901 caatccacaa gtacccatac cagaaccaat aggaatattc tccattttga atattgttgt
     9961 agataatggt ccaagtaata ttgaagctac agtaggtggt atccaaattt tccaattttt
    10021 tattatatta ggtacttgca acatagatgt cccaagacct tgtgccacaa ggccgccaac
    10081 tccattttct ctaaagctca taacagcaaa gcctaccatt tggcaacaac aacctacagt
    10141 tgctgcacca ccagcaagac cacctaaact taacatcata caaatagctg cactacttat
    10201 aggtaatgta agagctattc caacaagtgc agagactaaa gctcccatta ttataggttg
    10261 catagttgta gcttgcatta ttatatttcc aaatgaagtc ataaaagcag aaacagctgg
    10321 accaataagt tcagctacaa gtactccaat tagtattgtt acagcaggtg taaccaatat
    10381 atctattttt gtttcttttg aaactaattt tccaacctct acagcaaaaa gtgtagcgac
    10441 aaatgctcca acaggtcctc ctaattcatt accacaagct ccaacaagtg cacaagaaaa
    10501 tagtacaagt ggaggtgctt gaagtccata cgcaatagcc actgctatag ctggaccagt
    10561 aacctgttta gcaagagggc ttataacttc agtaaaaaga gaaatattga aattatctcc
    10621 taatgtattg aaaattgttc caattaaaag ggacgaaaaa agaccgagtg ccatataact
    10681 tagtgcgtca ataagatacc tttgaacaga aatttcaata ttttttcttt tagcaaatga
    10741 tttgaaattt ctttttttat aattgatttc caatttaaaa tcctcctcat ataatataag
    10801 tataatttct ttttaattaa ttatataact tttcagctga caagacaaat gttatttcac
    10861 taaaattaga ctattgtaat agtaagaaat taacattaat tttagtttta aattttaaat
    10921 ttatggttta taattagtat agggatattc tgaaatataa taattttcca gtttatccca
    10981 atatttaact taggtagatt gcttacaaaa caaatgatta tatattaggc taaaaggaag
    11041 tggatagatg atatttgtaa aaaataagaa atacagaaaa attgccataa ttatatctat
    11101 tcctatattg cttgttgcgc agtactacct ttttaaattt ggaatactta gaccaatggt
    11161 aaagggagta gaaattgaaa ttgtagatgg ggaatatgtt aaagatatag ataaatatgt
    11221 agtaaaatta aatgaaacag ttactttgtc tacaggtgaa tatattaagg ttccttcgta
    11281 tgctaaaaat ccaaatattt ggtttaatgt attggataac tcagggactt taaaaataga
    11341 tggaaataaa atgacagcat taaaggttgg gtattcttct gtgggtatca tgaaaaattc
    11401 aagagtatta aaaaaggcta ctataaaagt agtagaccca gaggtagaat cactagatat
    11461 ggagattaat ggggatttaa aacatgttgg tgatagtgct actatagaaa gtacaataga
    11521 agttgattat gataagttta aaaaatcata taagccagtt tataagagta gcaatgaaaa
    11581 tattttaaaa atcaaaggaa ataaagtaag tgcagttgga gttggtaaag ctacaatata
    11641 tgctaagtgt ggaaataaag aaattgagag aacatttagg attcaagcta gagttgctga
    11701 aataaatatg gatgatataa aaatagaagt tgaccagaat gaaaaattga aaccagatat
    11761 aataacttct ccacctggtt tagaaccacc aaaaatatat tatgagttag tagattcaaa
    11821 attacctgta gaaagagcaa taagtttaag tagtaatgga aatgttgtag ggcttagaga
    11881 aggtaaagat agggtgagaa taacttgtgg agataaatct aaagtaataa cagtaacagt
    11941 agtcaaagaa actataacga atgactatat acaaaacttg gttggttcac ataaaatagt
    12001 aaacaataaa gttattatta aacttgaatg ggattatatg aaagatgtct ttgattatga
    12061 tatatatctt aaagataagt tgtcaggaga atcatttaag aaaattaaat ctataactat
    12121 aaatgaaagt gatttaggta agtctaacaa ggtatatgca actatagaag tagatatgaa
    12181 gggtaatcaa aatatagatt ttgatattta tgtcataggg aaaaaggatt cagatacaac
    12241 taagccaagt aatgtaataa atattaccaa tggaaatgga aattcaaata atgataataa
    12301 tattaaagat atgagggtag aaaatatatc agctaatgtt gatagagata ataatgtcat
    12361 tagagttaac tggagtagta tatctcataa ggactgtact tacagtatat atgtaaaaaa
    12421 taatactaat ggagatggag aatttactct atatcaaagt gatatacagg gaaatgagca
    12481 tactataagt attcctgaag gagatttaaa tctagatatt tatgttgttg caaattataa
    12541 tggaaatagc tctcaaaata gtgatgttat aaatgtaaga tagatttgta tacaaattga
    12601 aagtgttgag tttatttaaa tattaaatta agaaaaagga tagtataggg taattatact
    12661 atcctttttt tgttataata tataggttta aaatctctag taatataaat tataaaaagt
    12721 cttatttaca caaatctata gatattcttt ttattaatca atattgtctt aaattgaatt
    12781 tagtgcagct tctattgcat gtattactgt cgtatcaaat agtggaatag aagtgtcatt
    12841 ttgtttaatt aaaagaccta tttctgtaca tcctaatata acagcctctg caccttgttt
    12901 gccaagctta tctataatag ataagaaagc tttttttgaa gattcagata taattcctaa
    12961 gcaaagttcg ttaaatattg tgtcatttac tattatacgg tcttcttcat ttggaattaa
    13021 gacttcaatt ccattattaa taattacatt tttataaaaa tcttgttcca ttgtatattt
    13081 agtacctaat aaggccactt tttttatttc tttttccctt aataccgtag cagttacatc
    13141 tgctatgtga agtaatggaa tatgtatact ttcttgtatt ttatctgata ccttatgcat
    13201 agtattagta cagataacaa taaaatctgc ccctgcctct tgtaatttaa ttgcagcatc
    13261 agctaaaatt ttggcacttt tttcccaatt tccactagat tggcattctt caatttcttg
    13321 aaaatctaca ctgtaaagta tacatttaga agagtgtagt ccacctaatc tttcttttat
    13381 tactgtatta ataacttgat aatatgtaat ggtactttcc caactcatac caccaattaa
    13441 gcctatagtc ttcatattat acctctctta tttatttatt tattttgaaa tagctattta
    13501 caaatacaag tatagcatct aaaatagaag catacaataa taaaattgta tggagtacaa
    13561 ttataattaa gaggagtaat tatatgccaa taaattcttt tgaaaactat cctatgacat
    13621 ggaaaccaaa attagataac cgaaaaccac caatatataa aactttagca atgttattag
    13681 aagaagatat taaaagagga aatttaaatc ctggagataa actgcctcca caacgggaat
    13741 tagcagattt tctagattta aacttaagta caataacacg agcatttaaa ctttgcgaag
    13801 aaaagggact aatatgtgca aaagtgggaa agggaacatt catttcttct gatgtaaatg
    13861 taagtaatac attattatat caaacggaat caaaagacat aattgaatta gggacagttc
    13921 atcctccata tgagcaaaat acatatatta ttgactttat taaaaatgta ctaaaacaac
    13981 cagaaatgga taggttttta caatacatgt ctccatctgg cacatatatg caaaaaaagt
    14041 caattgcaaa atggatagaa agaaataatg tatacaccag tgaagaaaat atattattat
    14101 ctacaggagg gcaaaatgct atatgtgcaa ctttactagg attatttaaa gctggtgata
    14161 gaattgctac agattcttta agtttttctg gaattaagtc aatagctaaa atgataggta
    14221 ttcaattagt cccaattcct caagaaaata atgaaatatc gattgaatat cttgaaagtt
    14281 attgtaaaaa tgaaaatata aaaggcatat atcttatacc agattatcat aatccaacga
    14341 cacatactat gtctgattta tctcgtaaaa agatagccaa gatagcaaag caatataata
    14401 taataattat tgaagatgct ataaatagca tttttagaga tggtatacaa actcccatat
    14461 tttctttagc tagtgataat actatatata tttttagtac atctaaattt ttatgtgctg
    14521 gtcttagagt agcatttgta gttgctccaa aacgatatat agaaaattta gaaaatgcac
    14581 tatataatat gaatctaatg gtttctccat ttacagcaga aatagtacac cgtttattat
    14641 attctcctat tatagataaa attatagaag agaaaagaga tgcaattata gaaagaaatg
    14701 aagttgctga taaagttttg tcagattata atttgattgg tgataagaat tgcagttttc
    14761 gttggctact tcttcctgat gaattggatg gaaaatcatt tgaaatttct gcaaaaaatc
    14821 taggagttca agtatattgt gctgaacgat tttcagtagg aaactctaca gttccaaaag
    14881 cagttcgtat ttgtgtaaca gctccaaaag atgtagaaga attagaaaaa gggcttaata
    14941 taataaagtc acttcttaag agttaaattt attttactat tatttaatta aataaataaa
    15001 aaggcggaga aatttattat aataaacctc cgcttttatt atttaatgaa atactttact
    15061 ttttattaaa aaatttaatt ttaataatct tgaaaatata atcgttttat ggtaaaataa
    15121 ttcttaaaaa tagaaaaaat taatcatttg ttaaaaaaaa tgcataattt attgtaattt
    15181 attgacgaag gttgaaaaat attcataaat gtgcgaaata acagaaaaaa tgcaaaaatt
    15241 aattcaattt catttgttat aaaatctata tggcaaaatt aaatctgcct atcagaatga
    15301 tataaacaca tcagaaagga ggacgctatc aaataataca agtagttaac tttgatagca
    15361 aaaaattatg aatgaacaaa ctagaatatc cttagagaga gctgctgaat taaaatcaaa
    15421 aattgatgat tatattcagg ctagaaaaac gattaacaga ggtcttgaaa aagaagaaga
    15481 gataaataaa cgaaaacaga aaatattaag tatcttaaat ggaactgaag aggattggaa
    15541 taactacaaa tggcaattat caaatagaat aacagatgta gatactttat caaaaattat
    15601 aactctaact aaaaaagaaa aagaatatat aaaagaggtt ggtactcaat ttagatgggc
    15661 aatatctcca tattatttga gtcttataga cccagaagat atatgtgacc caataaaatt
    15721 actgtctata ccaacacata tagagttgga agatgaacaa gaagatttgg acccaatggg
    15781 agaagagtat acaaacccag caggatgtat aactagaaga tacccggata gattaataat
    15841 aaatgtaaca aatgagtgtg ctatgtattg tagacactgt cagagaagaa gaaatattgg
    15901 acaacaagat tctcataagt caaaagctat tatccaagaa tctatagact atatcagaga
    15961 aaatgaagaa ataagagatg tactagtaac tggtggagat gctcttacat taaaagatga
    16021 ttatttagag tggattctta gccaacttaa agagatacca catgttgatt atgttagatt
    16081 aggtactagg actcttgtta caatgccaca aagaatcaca gatgaatttt gcaatatgct
    16141 aaaaaaatat caccctgtat atataaatac tcattttaat catccaatgg aaataactaa
    16201 ggaatctaaa gaagcttgtg aaaagttagc aaatgcagga gttccattag gaaatcaggc
    16261 agtattatta aatggaataa ataatgataa atttgtaatg agatgtttaa atcaagaatt
    16321 actgaaaata agagtaaaac cttattatat attccaaagt aaacatgtaa agggaacaaa
    16381 acatttcaat acatcagtag atgatggtct tgaaatcatg gagtatttaa gaggatatac
    16441 atcaggaatg gctataccaa catatatagt aaatgctcca aaaggaggag gaaagactcc
    16501 tttgcttcct caataccttg tatcaaaagg aacagattac gttatgctta gaacatggga
    16561 aggaaaagtt ataaaaatgg aagatgaacc tgctgtagat ataaagaaac ttataaaaga
    16621 gcaagcacag gactaaaaaa gtatttaaag tctataaaaa gaaacaactt taaaataaaa
    16681 agaaaatgga tgccgaattt atatcaattt gcatccattt tttattagtt atttattatt
    16741 ttgttgttga ttaactaatt caagatatcc acttgggtgg aagtaatata tctcagaagt
    16801 attttcattt acaaagtaac caatatcacc atctgcatct ggtaaaaata cataaccatt
    16861 aagattttta gcttttaatg cttcaaaatc actagcattt ccttgatata tgtaatcaac
    16921 ttcattgttg tattttacta aaagttcttt agcttgctct tgagtcaatt tagtattttg
    16981 gcttacagga acattctttt gagaatctgc ataagaagat tcatttactg gtgcagttaa
    17041 taacaaacaa gataaaacta ataaagatat aagtgctttt ttcatactaa aaccctcttt
    17101 catgtttaat tatataagat tcattactta ataatattgt atgtttccta taattaaata
    17161 ttaccattaa agagaataat attacagtta cacagctgta acttttgatt acaaaatcgt
    17221 aattaaattg tatttttatt ccatttgtat tttatcatgt ctacgattta caaattcaca
    17281 gaaatatatc tttataacac ccatcacagg agcagataga atcataccag gtataccaaa
    17341 gaatcctcca ccaacagtaa ctgctaggat tgtaaagaat gggctaagac ctagttgtcc
    17401 acctacgatt tttggttcta taaatgtaac ttcaacttgt tgaattatta ttaagtaaat
    17461 taaagaagtt aaagcagttg ttggattgga gaataaatta attattacaa ctggtgccat
    17521 acctattact ggaccaaaat atggaaccat attcatgaca cccataagtg taccaaataa
    17581 cagtgcatat tcagatttta aaaagtaaag accaattcca gaaagtactc caactataaa
    17641 agaatctaaa atcttacctg taaagtattt accaatattt aaatttaagc tttgacatag
    17701 ttctattata aagtctccat attttttacc taaggaaata taaactactt tttttgtaaa
    17761 cagtaagaat ttttcttttt ctaataatat atagaaacaa attataaaac ctaaaagaaa
    17821 ttgtactata aattttccaa ctgaaaatgt agtgctaaat atcccgccaa gtgaaccaat
    17881 aagcaagtta ctaaatttag gcattacact cataattttg tcaccatatt cttttaatgt
    17941 attagggtca actgattgta agctcttgcc taagtcaaat aagaactgct ctgttttatc
    18001 tatgtatgta ggaatttgag caactaaatc agctaaacta tttactatgg atggaactgt
    18061 aaataatata aaggaaatta gtataactat gagtacacca taagtaagaa gtaatgaata
    18121 tattctctta aaatttaatt tactttctaa aaactttact agtgggttga atatatatgc
    18181 taatacaaat gctattataa atggtgttag tagtgacatt aataaactta tcactccaaa
    18241 aaaatatttg taattatcaa taactttaat caagatatat gaaacaacta cagtgagtac
    18301 aatatttaga taaatttttt tatcttgcaa aataacacaa cctttcttat atttattaaa
    18361 tttagatata cttaaatcta agttaaagtt aatatttata gcttattata caataaaaag
    18421 cagtattaat atagtat
//
LOCUS       DAEORZ010000081        18401 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEORZ010000081 DAEORZ010000000
VERSION     DAEORZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.081"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(228..758)
                     /locus_tag="KRH39_002389"
     CDS             complement(228..758)
                     /locus_tag="KRH39_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8512288.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            complement(846..1940)
                     /locus_tag="KRH39_002390"
     CDS             complement(846..1940)
                     /locus_tag="KRH39_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBG8512289.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDISIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHHYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            complement(1942..2388)
                     /locus_tag="KRH39_002391"
     CDS             complement(1942..2388)
                     /locus_tag="KRH39_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG8512290.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            2694..3884
                     /locus_tag="KRH39_002392"
     CDS             2694..3884
                     /locus_tag="KRH39_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG8512291.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIDTFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            4054..5016
                     /locus_tag="KRH39_002393"
     CDS             4054..5016
                     /locus_tag="KRH39_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG8512292.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(5138..5800)
                     /gene="zmp1"
                     /locus_tag="KRH39_002394"
     CDS             complement(5138..5800)
                     /gene="zmp1"
                     /locus_tag="KRH39_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBG8512293.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(5900..5985)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(6170..9088)
                     /locus_tag="KRH39_002395"
     CDS             complement(6170..9088)
                     /locus_tag="KRH39_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG8512294.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEVPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSKIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSVLEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDANFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPVPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(9206..9291)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(9418..10596)
                     /locus_tag="KRH39_002396"
     CDS             complement(9418..10596)
                     /locus_tag="KRH39_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG8512295.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            10971..11120
                     /locus_tag="KRH39_002397"
     CDS             10971..11120
                     /locus_tag="KRH39_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG8512296.1"
                     /translation="MFFISYIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCV"
     gene            complement(11221..13995)
                     /locus_tag="KRH39_002398"
     CDS             complement(11221..13995)
                     /locus_tag="KRH39_002398"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG8512297.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(14167..15423)
                     /locus_tag="KRH39_002399"
     CDS             complement(14167..15423)
                     /locus_tag="KRH39_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG8512298.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(15495..16997)
                     /locus_tag="KRH39_002400"
     CDS             complement(15495..16997)
                     /locus_tag="KRH39_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG8512299.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYSDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(17088..18278)
                     /locus_tag="KRH39_002401"
     CDS             complement(17088..18278)
                     /locus_tag="KRH39_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8512300.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
BASE COUNT         5785 a         3175 c         2160 g         7281 t
ORIGIN      
        1 ctaattatct tttataagtt ttcatacaat aatatctcct ttcaataata tatctttttt
       61 tatatttaaa actatcttat tttctataaa ataatgtata aacaaggttt acagtcaagt
      121 atttttatta tttttttgat ttcataaaaa aagcatagca aattatttgc tatacttttt
      181 tactaaatta tttattctat atcttatatg aacttattgt ttaactacta cacaatacta
      241 gctttaatca tcatattttt tgcaaacata ctggcttcat atatttcttg ttcatctgga
      301 tgactttgag attcctcatg gttatttaat tgttggcgaa tatgttcgtt gtcaggagaa
      361 agttttaaca tctctttata ttttgctttc aatccttctg ctatcttacc ttgacatatg
      421 aatgaaccta atatttcatt atcttttggg ataattgatt ctatcttttc tattatacta
      481 gctccataac ttcctttttt acttgctccc attgttccaa atagtgctat ctttttattg
      541 tgaatatttt caagcacttg ttttgaattt ttatcacaaa ttcccttgtc cacccaatag
      601 cctaagaata ctatatcata attttcatag tcaatactat aatcaactaa ttcatttaca
      661 tctttaatgt ctttttcttc ttgaatttca tcatatatag tataagcaat ctttttagta
      721 tttccagtta aacttgaata taaaactaaa cttttcatga tgtctcctcc tctttatcta
      781 aatacttatc taaatcacca aaaatatatt aaccttaaca aattatttat ttttataagg
      841 atattctaat ctagtataca tccaaactga gagcgtgttt gtaaagcatc tgcacacttc
      901 ttattacaat aacagcactt tttaatctca tctactcttc catcttttac tttctgtaac
      961 caattactat ctgctagtaa ttgtcttgac atagaaacca tatctactct attatttgaa
     1021 ataatacttt ctataaaatc tgggctagac aattttccaa ccccacatac tggaagattt
     1081 gtgtgtttct ttacctcatc agctaaatat aaaaaacagc cttcatcttt aaagtaatga
     1141 tgattattgg ttggtatagt atcttctaat tttgaatgat ttgcaagtgt tacatgaaaa
     1201 ctgtttacac ctagggattc aaatttttta acaaaatact caacttccga aagtaaaact
     1261 cctgcatttc cataatgagg attttcttgt cttattgcaa atttatagtc tattgaaatg
     1321 tccttaactt catctcggat actactaatt atttcgcttg caaatctact tctattatca
     1381 cacgttccac catattcatc atttctatta ttaaatatac ttgatgaaaa acttcctaca
     1441 agcctatcac catgaatttg aatcatgtca aaacctgctt tttttgcatt caatgctgtt
     1501 actttaaata aatttataat atttttaatt tcttctttgg gcatatttgt tatgtaatct
     1561 tttactccgt cattcattat tttttttatc tcatcatgtg aagtaattcc catcttcata
     1621 gtatctttaa ttaaattaac atcataatct gaacaaaata gttgtgctga tacctttgca
     1681 ccagaattgt gtatttcatc tacaattttc ttgtatttca tgactccatc ttcatccgat
     1741 aggcttatag ctaccttatg aaaactaggt ctaatagata catctcctag agtaatcaga
     1801 gctactcctg acttagcaat atcagaaaac tttttaattt tttcttccaa tttaagaccc
     1861 atgcttgtag gtgcaaaaga tactctattt tttaattcca aatttccaat tttaattggt
     1921 tttaataaat tacaatacat cctattcttc ctccacagct gtttttatat cctttaccac
     1981 atgtaaattc tttaccagtt ctttatattc tgtatccgag aactcttttt ttatgtgatt
     2041 atcccacttt gaaaatatgt tttttatatc ccctaatact tcctcaccct tatctgttac
     2101 tctcaactga aaagctctac tatcattagg gttttggatt ttttctatgt atccatcttg
     2161 ttctaaccta gatatagttc ttgttgtatg agctttatca acttcaaatt ctgtagaaac
     2221 atcattcaaa gaacaattcc tatttttatt aatataaatt aaatagaaat atagaccatt
     2281 cgtaagttta tatggtttta aacatttttc acaataaagt ttaaagtttt tttgcaagtt
     2341 acaaataata tatgacaaac tttcatactg taaattttta aaatccatct taaatttccc
     2401 ttctataatt ataatatgtt tgactatatc aactatatta tattaaacaa ttaatatagt
     2461 caagaatatt ttattctcta aatctatgtt ttattaattc tttaaaaaac tattatataa
     2521 aataatcatg ataaaattag aaattggaat ttttaatatg cactgtacat aaatatattt
     2581 tatataaaag tttatatttt caaacaagta atttttcgaa aattgtctaa attcagtaaa
     2641 aatatggtaa tatatacata agcaatataa atctataaaa aggagtgtta catatgatat
     2701 ctaataaaat gcaaacttta gttgcaaata gttctgttat aagagctatg tttgaagaag
     2761 gaaagaaact ttctgatatt tatggtgagg aaaatgtttt tgattttagt ataggaaatc
     2821 caagtgttga acctccagag actattaaag cagttataaa tgatatttta aatgaagaat
     2881 ctccaaattt agttcatgga tatatgaaca actcaggata tgaggatgta agagatgcta
     2941 ttgctgaaca cataaataaa aaagatggat taaatcttac aagagaaaat ttaattatga
     3001 cttgtggtgc tgctggtggt ctaaatatta ttttaaaaac tcttttaaat cctggtgatg
     3061 aagttattgc ttttgcaccg tattttggtg agtataaaaa ttatacagaa aactatgatg
     3121 gaaaattaat agaagttcct acaaatatag atacttttga gccagattta gatgctctaa
     3181 aaaatgctat tactccaaaa acaagagctt taataataaa cactccaaat aatcctactg
     3241 gtgtcatata ttctgaagaa ttattaaaaa atcttggaga attattggat tcaaaacaaa
     3301 aagaatttaa tacaagcata tacctaattt ctgatgagcc atatagagaa ataatttatg
     3361 atggtgccaa agttccttgt gtactaaaat actatagaaa ttcatttata ggatactctt
     3421 atagtaaatc attatctcta cctggtgaac gtataggata tattgttgct aatgggcaaa
     3481 tggatgattt tgatgatgtt atgtcttcat taaatgttgc aaatagaata cttggatttg
     3541 taaatgctcc ttctcttttt caaagggtta ttgcaagaag tttagatgca gaagttgatg
     3601 taaatattta taaaaagaat tttgaccttc tatataatag tttaatcgat atgggatatt
     3661 cttgtgttaa acctaatggt acattctatt tgttcccaaa agctccaatt gaagatgata
     3721 aaaaattctg caatgatgca aaacaattta atttattatt agtaccaggt tcttcttttg
     3781 gttgtccagg tcattttaga gtatcatatt gtgtatctta tgataaagtt aaaagttctt
     3841 taccagcatt tgaaaaatta gccaaattat ataatttaaa ataactaaat caaagtaatt
     3901 caaataatta actaccttag tatagaatac ttacattcta tattaaggta gtttataatt
     3961 ttatgtacta tattttatac ataccatatt attccaatat gcttgaatat tcttatctat
     4021 agtactaata taaattacaa ggaggtgttt gacttgaaaa aaggttatgt ttatataata
     4081 ctgactacct tattttttag ttcaatggaa attagtctaa aaactgtcac taatgacttt
     4141 aatcccttac aaataacttt atcaagattt tttgttggcg gtttagtctt actacctttt
     4201 gctgtaaaaa gattaagagc tcttagtctt tctattacaa aatcagactt aaaatacttt
     4261 gcatttttag gttttatgtg tgtagtagta agtatgtcat tgtatcaact tgctgttcta
     4321 aacacaaagg catctgtagt tgctgttttg ttcagttgta atccagtctt tgtaatgctt
     4381 ttagcttact taattttgaa ggaaaaaata tataggcaca atgtaatttc acttatatta
     4441 gaagttcttg gaattatagt tataataaat ccattgcata caaaactaac tcttagtgga
     4501 atattcttaa cattatcttc tgctattatt ttttcaatgt atactgtatt tggtaaaaga
     4561 aaaaccttaa aatttggtgg aatagttgta acctgcttta gctttatatt tggtagtttg
     4621 gaaatgttga tattggtttt actcactaaa attaatttta ttgcagatat attaaataaa
     4681 aataagcttc atgtatttgc aaatatccct atttttagtg gatatacctt acacaatatg
     4741 cccattatga tttatgtatt cgtatttgtt acaggtgttg gttatgcgct ttactttatg
     4801 gcaatggaag ctacatctac tagtcttaca tctttagtat tcttctttaa accagtaata
     4861 tctccaatac tagcattatt aatacttaaa gaaattatcc ctataaacat ggttatagga
     4921 attttattga tagtaattgg ttcaataatt tctattatac ctacgataat tactcaaatg
     4981 cataataaac acgttgaagc cttgtataag gagtagtaat tgataatcta tcatagttat
     5041 acataagaca aaatttatta gatttaaacg caatataaaa gagatgctat tataatagca
     5101 tctctttatt tatatttatt ttaaatttat taaaactcta tttagctaaa ttttgcaaaa
     5161 agctataagt ttgtggacaa gctgatttta acttactatt tgtatcttga tttaaatagt
     5221 aatatgcaaa tgattctgca aaaaattctt ctggatatac acctaagtag tttacattac
     5281 ctaaagaacg accttcttta gcaaatattt gtttgaattg tgctgattta gatatatcat
     5341 ttaatactat atggtctatt gcatgtgctg tttcatgtaa ttctaaattt attgcatcat
     5401 gccctttacc tttgttacta aatcctattc taagagctac tgtacttcca cctaaacctg
     5461 gaacatcatc ccaagttttt ccagtacctt cccatccctt aggaactaca ccttttaaat
     5521 aagcatattc tttttcatct gttaactttc cacttaataa ttttattttt aagttatttt
     5581 gttttaatgc atttaaatac tttccatcta tattagctaa tctatttacc atagcattag
     5641 cttcgttttt atcgtaattt ccagttggaa aaactactat ttgactaagt gtgtctttat
     5701 tttgttgtat agtagtacta tctgcatgtg cacttactgg aactgaactt attaagaata
     5761 ttgatattat agctattaat aattttttac ttggtctcat attattccct cctttgagtt
     5821 aggccgggag taataaattt caaaataaaa aagataccca agagtatctt tgtatataca
     5881 aaaagaaatt tttactcttt tcggcaactg gctggcatgg tttactaaac tttagcccct
     5941 atagctttgc gtgactaggt ttccctagtt ttgcctcatt taattttata tctatattat
     6001 agcttatatc tttttaattg tctatatccc tttggaactt ttttctaaaa agttattatt
     6061 tttttacata aatcttcaaa aaaaaatatc ttctaacaat attttcatta ttaaatattg
     6121 ttttagaaga tattttttaa tataacttat atttttataa tgttagggtc taatttgtat
     6181 ttttatttct tcttaatacg ataagtccta caatagctcc tataaccaaa agtatttctc
     6241 caataattgt agtactatcc cctgtttttg gtggtactgg tggatttacc aaagtatcat
     6301 ctttaacttt attatttttt tcttctgaca ctgatggctt tactacttct ggtattggaa
     6361 taatctgttt aacaggagtt tctatcatct cttctgatgg tggtactggc ggattcaata
     6421 cctctggtat tggcggattt attatatctt ctgatggtgg tactggtgga tttataacta
     6481 tagtatcctc tgtatcagat gaacttgatg gtttccttgg tttatctgtg cttggtggta
     6541 ctggtggatt tactattggt tcatctgtat ttggtggtgc tggtggattt actggtggat
     6601 tttcttgtct tatgttttct atatttccaa tatccactat tctaccaata tctttattac
     6661 caatttcaac cttaatttta tccttcagtt tttcatatcc acttggagcc attatctcca
     6721 ctaaataata tgttcctggt ttttttatac tagattgaat tataccatct tcatcactaa
     6781 ctaaatcctt aactactata tttccaaatg catcttctaa attgaactta gcacctgaaa
     6841 gtacattttt atctaaagca tccacttttt tagcttcaaa agcataacct tgcttagtag
     6901 tctctactcc tggaggactt gaaccattaa tctgagccac atttcgtata gtctccccaa
     6961 cttttacatt tgtgaagttt gcatcaaatg taacccaact ttgcacaggt tccccttctt
     7021 ttatctgttt aaattcgtat ttatttacta atcttattgt attttttcca tctccagctt
     7081 cttttttaag ttcaagatta tcagaatatt ttatattaga tatttctaga actgagttta
     7141 cttcatctaa aagatttact tgtcctgcat cataaactcc ataagttgac atattgattt
     7201 tgtatttaac aatctggtta tcactactat ttaaaacttt tttatcaact tctttttttg
     7261 cgcttattaa tccagaattt ttcttaaagt ttatctcatc atttttatca attttactat
     7321 tatttgcatc aagaacagct ctatttatat agctatcttc aatactaatt acctttaatg
     7381 tataagctat aaaaaacttt gattccactt cttctttact gagattaatt gtaaatccag
     7441 ttataacttt atctacttgc ccactttcat ttcttttttc ttctacttta tatgaaatat
     7501 tattggcaat tttttcttta ctccaaggag tccagctccc attcattgga acttctacaa
     7561 ctctaaaatc atgcccagct tctcctgaag tagttaatct catgccttct ggtatattat
     7621 ctgttataac aacattactt attggtgatt tatcatcatt tattgaaatc tgatagttta
     7681 ttacttgacc aacttgagta atatttgttg ctcctgaatg tcctttagta agaagataat
     7741 catctcctct atcaaatata cttacagtag catcatcttc ttgtatcttc ttaccatcag
     7801 attcaatcct agctacattc ttatatttat taccatgttt tgcatttttg tcactgattc
     7861 tagccttata cttaacagta tacgatttat ctatatttcc aaaattaact gaaatagaat
     7921 ttttactctg ttcaatatta aaatcactaa cttcttttcc atcacttgtt actacactta
     7981 cagaaccatt tatcaatgtc aatccttcag gaagctcatc atacaaaata atatctttta
     8041 actcttcctg cttttcattt acttgtatac tgtaatttat atctttattt ttaactggca
     8101 taaatacagt agttgatttt tttccttcaa caagcttttt aagtgttcct ttagcatctg
     8161 gaacatccgt tttatctcca ggttttattt ctacatctat atctccactt ccactttcta
     8221 tttttccagt agtttcaata tgtatttttg cattagtatc ttcatcaacc tttttaacat
     8281 atgcttccaa acctatttta ccttgcatta cactttgacc attaggtaat tctctaaatg
     8341 taagtgtata ctctccatta ctttctgttt tatcaaagca ttcacttgta taatctaaat
     8401 taactttatt gaatacatct ggcaccttaa aagttattga atctcctgct tgtattcctt
     8461 ttgatgttgg agtataagtt aaattaaatg atattttttt cattgattcc gtttctattg
     8521 tattgttatt aatttttaca acaaatccat agtcttcatt ttcttcatta agttcttctt
     8581 ctaaaacttt caataaattt tcatcttcta atagattatc ttcctctgga gttttttctg
     8641 tatttttatt gtctagtggt ttttcatctt ctaaagcttg ttctgacttt ttattatctg
     8701 ttgacttatc ttcttctggt atctgctctg gctttttatt atctattggc ttttctcctt
     8761 ctggagtttg ctctggcttt ttattatctg ttgacttatc ttcttctggg gtctgttctg
     8821 gttttttatt atctgttggt ttttcttcct ctggggtctg ttctggtttt ttattatctg
     8881 ttgccttatc tttctctgga gtctgttctg gctttttatt gtcgctttcc ttctcctctc
     8941 cagttgatga attagttgaa ccactttgaa tctgactatt ctctcctaat tctgaagcat
     9001 aagatacagt tggatatatt gtagacacca ccatacttaa aatcataatt aaactaatta
     9061 ataatgcctt tctatttcct ttcttcatgt aaatctcacc cctttaggct acctataatt
     9121 tataatctaa tcttccatta atatcgtata taaagtaatt tttactaaaa aattatacaa
     9181 aaaaagctat gaaaataaat aaatttatat tcatagccgg ttgcaccact gactcagtat
     9241 atccataagg tgcccaataa tagaatatac ttcaacatct ctataatatt tcataaaaac
     9301 aattaaaatt gctttattta tattaaaaat acaattatat acttctatta tattatgcct
     9361 atatactatt gtctatcctt tttacatttt ttaccttatg atgagattaa ttaataatta
     9421 aataaaatcc ttatctacaa caattacaca attatattca ggatttatct ccttaactaa
     9481 ttttgttata tcttctttta agtcatcttc tgacatgttt ttggaaaatt cggaaggatt
     9541 aactactata tcaaatatta aattcttctt gcttcctttt ccaactattc taaaatcatg
     9601 catagatttt acaagactat ttttcttaag tatttcttca agttcttttt tggctgacat
     9661 aacttcttta tcttcaagac atattgggtc catgtgaatt actagatata tatctaattt
     9721 ttcagatatt tctctctctg cagtatctat aatttcatgt atttccatta tatctatgtt
     9781 ggatggaatt tctgcatgta tagatgcaat acatctacca actccatagt tatgaataat
     9841 taaatcatgt gtacctatta tatatttata agataacaat agctctataa tagaatttac
     9901 catttcttca tccggtgctt cccctaaaag aggatttata gtatctttta caagagagaa
     9961 accagcataa agtattacta atgatactac gataccaaca tatccatcta tgggaaaatt
    10021 agtaaatcta gctacaataa atgatataac tacacaactt gaagtaaata catctcctaa
    10081 agcatcaagt gaaactgctt ttaaggctga agaatcaatt ttatttccca taaatttatt
    10141 aaaacgactc aaccagattt taatcattat agatgcaatt aaaagtataa gtggtattac
    10201 ttcaaatttt acaggaattg gatttactat cctttctata gacgacttta caaattgaag
    10261 tccaaccaac atcaccataa atgctactat taatgctgat aaatactcta ttcttccatg
    10321 accaaaaggg tgttctttat cagctggctt acttgctaat ttaaaaccta tcatagttat
    10381 tgctgaagat gccatatcag aaagattatt aaatgcatca gccattatag ctatactaga
    10441 tgtgagcatt cctatgaata cctttatcac aaataaaagt aaattggata atatacctac
    10501 tacaccagct acataaccat acttatttct aacatctgta ttttgaacgt tttcgctatc
    10561 tctgataaat gttttgacta ggattttgga aaacatgaaa cttacctcct tgccctttat
    10621 tttattaaca aattttataa tctatatctt tatttatgat aacataattt atatataaaa
    10681 ttcttaaaaa tcactctact tcaataaaat attattctat atatctattt agatacgaat
    10741 tttatattta gtaaagtttt tatggcttag tgtctgttta atagattcta tataagtttt
    10801 tacaaagatt atttattatg ataatatcat ttttcccaag tattacttag aatatgattt
    10861 ttttgttttg tctgtacata ttttaaattt atactatatt tatctatatt ttataacctt
    10921 atgatacact ataactataa gtttttaaat ggaaaggaat gaaataaatc atgtttttta
    10981 tcagttatat ttacacaaaa tcacctatta aatttgatac tcctttgcaa aaagaggcat
    11041 acaaaatatt acaaaaatta gatattgact ttgaatgtgt tgacacagat gaagcaatta
    11101 caatggaaga ttgtgtttag attaataaaa aattaaatat gaaaatggta atataaaaca
    11161 acattaaaca aacaaaaacc gcaaagttac aatatatagt actttacggt ttcataagaa
    11221 ttaattttta ctattaaagt tcatagttct actatttttc attgttctaa gaattagctt
    11281 tacaaattca tttgacaata ggatagtgga tgcaaatagt attatcttta tccacatatc
    11341 aaagtccaaa gaaacagaat taaagaaact ctggaacact tgtgtaaata ttatttgaat
    11401 tatccctgta actactatta tttgcaatgc cagtttattt ttaaagaaat ttggtattgt
    11461 actatttaaa ccaaactctc tacaatttaa tgcattaaaa agggcactga atgcaaataa
    11521 agaaaatata acagtacctt gttcttctga tgtagctccc aatatattaa aagctgattg
    11581 agtaaataca attgtaatta taagaatagc atttataata atatttacaa acatagacct
    11641 agctattatt ccagaatgtc tatttattgg ttttcttttc aatacataat ctctaacagg
    11701 ttctaatcct aatgctaggg ctggaggacc atccattatt atatttaccc ataataattg
    11761 tattgtagta aatggcatat ccttaccagt aagttgagat attatagcaa ttataaatgc
    11821 tactatattt acagtaagct gaaactgtat aaatctttga aagttctcat atatacctct
    11881 tccccatttt attccctcta ctatagtact aaagctatca tcagtcaata ttatatctgc
    11941 tgcattttta gatacttctg ttcctgaaat acccattgca attccaacat ctgcttgact
    12001 aagtgctggg gcatcattta taccatcacc tgttacagct acaacttcgc cacttttttg
    12061 aagtgctgaa actattctca tttttgtatc tggtttactt cttgcaacaa tagatattcc
    12121 tttgatttct tctctcaatt ctttatctgt caatacatct atatatgttg cttcaactgc
    12181 ctttttccca tcatttaata atcctaattc atttccaata gctgttgctg tatttatatt
    12241 atctcctgta agcattttta catctactcc tgcattaaat gctttatcaa tggaatcttt
    12301 gaccccttct ctcaatgggt ccactattcc tacaaatcca ctaaaaacta aattatcttc
    12361 cttcatataa cttcttgaat ttcctattaa attcaattca ccttctgaag tcattgcaac
    12421 ttctgtttta gaattactca tttttttata agcaaatcct aatgttctca tagatttaat
    12481 ttgtaacttc ttaatctcat ctaaaatact tttttctact tttggagtta ttggaacaat
    12541 attttttcct tgttgtacat aagaacattt ttttaataat acttctggag caccttttga
    12601 aagtaatata tctgaatcct cttgacgaat taatgttgac atcttctttt cttctgaact
    12661 aaatggtatt tgtgatatca aatacgtttg ttttctcatt tcattatagt tcttatcatt
    12721 atgatataaa agtaatgcac actctgttgc gcttcctata tatttaaatg aattatcttc
    12781 tttttctata tctgcagttg aatttactat acagttttgt tcaaaatagc tattactttg
    12841 atactctcct ccacttatat atttaccatc aacatatgct acctctacca tcattttgtt
    12901 ttgtgttaaa gtccctgttt tgtcggaaca tattacagaa acagaaccta tagtttcaca
    12961 ggcttctttc tttgttacta atgcatttat ctttgccatc ttttgcattg ttatagctaa
    13021 ggtaatattt atcatagttg gcaaaccttc tggaacagtt gcaactatta atgcaataca
    13081 aaccatatat gcatttttag caggttctaa tgattgtaag aaagataata cactgcttgt
    13141 atctaaaatt aacatacctt ttctctgcat ctgtataatc ataaacatac acaataaagt
    13201 agctattatg cctgatattc ctgctatttt agcacccaac ttccctaatt taatttgaag
    13261 tggagtttgc tggtcttcat ttgataaatt ctgtgctatc ttacccatct ctgtcttatc
    13321 accagtatga gtgacaacca ttatgcccct accatatgct acaagagtcc ctccaaatac
    13381 catattgact tgctttgcag gtattgcatc ctgttcgatt atttctgttt tagaataaac
    13441 tacttccata tctaatacag catcagcatt cttagctaca tcatcagatt ctcctgttag
    13501 catatcttct ctaagtttca aatttataga ctgtataagt cttccatctg ctggtatcat
    13561 atccccagtt tctatatata caatatctcc tggtactaaa tcattttttg atattttaat
    13621 tatcttccca tttctaagca cctttacttc tatattttct gttaattttg acagggcatg
    13681 agctgctttt ttagattttc cttcagttat tatgcctata gaaattccaa taaaaatagc
    13741 ccctaaaata cctaatgcgt catgtaattc tcccacaaaa gaacttatta ctgctgctcc
    13801 tatgagaatt aatatcatag gttctgtcaa actttctccc aactcgtccc aaaatgttct
    13861 accttctttt atagtaaatt catttaatcc atatctaagc ttcctttctt ctacctcatt
    13921 atcatcaagt ccaatttctg gatttgtttt taagtatttt aatacttctt tggtaggttt
    13981 attataatac ctcataactt tatccccctt gttaattatc tataaggaat tatatgctta
    14041 tgatagcttg tatattactt ttcaaaaaaa ataaggtgtt gattaatcaa caccttatag
    14101 tggttatata ttattaaatt ttatgttaca ggctttataa attgtgagct atacaaatca
    14161 aaatatttaa tcttcaaaat acccttctgg atataatgga aacttagata taagttcttg
    14221 tgcctctgct ttacattcat ctaatacttc tttattatca atattttctg ctaccttatt
    14281 cattatccct gctatctgta taacttcttt ttctttcaga cctctttgag atattgatgt
    14341 aagacctata cgaacaccac tagttacaaa tggtgattgt gggtcgtcag gtataacatt
    14401 tttattaact gttattccta catattcaag agctttttca aattgttttc ctgtaagatt
    14461 cttacttctt aagtcaacca ttactatatg attatcagta cctccactta caatactaaa
    14521 accatgtgaa atcagttcag aagctagagt ctgagcattt actacaactt gctccattat
    14581 ctttttaaat ttatctgtac ttaaatataa tagtgaaaag gcttttgctg caactgtatt
    14641 aagatgtata gaccctaatg ttcctggaaa aacaccttta tcgagtttct ttgcatgttc
    14701 tgctttacaa agtacaatac cacttcttgg tccacagaaa gtctttgtcg tagatgaaga
    14761 aacaaaatct gcataaggta caggactagg tattactttc gctgcaacta atcctgcaac
    14821 atgtgccata tctaccataa agtaagctcc tacttctttt gcaacctttg atattctttc
    14881 ataatctatt aatcttggat atgaacttgc ccctgcaatt ataagttttg gtctacattc
    14941 ttttgccttt gcttcaagag catcataatc tatacgtcca gtatttgggt ctaccccata
    15001 aaattcatat ttataaatgt catgtaaaaa attaactgcg ctaccatgtg tcaagtgtcc
    15061 accttggtca agacgcatac ttaaaacagt gtcatttggt tttaatattg atgaatatac
    15121 acaatagtta gctgtagaac ctgaatacgg ttgtacgttt acatgttctg ctccaaatac
    15181 ttcttttgca cgttttattg ctagggtttc aagtttatct gcttcttcag aacctgcttg
    15241 aaaacgagcc cctggatagc cttcttctgt tttatttgta aatacacatc cacttaattc
    15301 aagtacttct gttggtgctg tactttcaga agctatcatc tcaatattgt gttcttgtct
    15361 aaccaactca tcagctacaa tcttgtataa ctctgggtct gaaatttttg ttgtttttaa
    15421 cataataatc ctcctcttaa ttaaagaata tattttatta gtttaatctt cattgataaa
    15481 tttattataa cggtctataa aacgtaagat attcctccac caggtcctaa tggtatgcca
    15541 atacccatga atagtaacaa tattattatt tctattaatg taaataccac tgagtatgga
    15601 atcatattcg atatgacagt acctatacct atatctttat catatcttct ggcaaaggcc
    15661 aatataactg gaaaatacgg aaaaagtgga cttattggat ttgttgaagc atctccaata
    15721 cgataagcta tttgagttaa tgctggatta aagtctagta gcataaacat gggaacaaat
    15781 ataggtgcta aaatcgccca ttttgcagaa gcacttccta caaataaatt gataaatcca
    15841 gatagcaata taaatcctat tatcaatcca tagctaggta tccccgactc tgctagtaat
    15901 tttgctcctg ttattgataa aattgttcct aagttactaa gattaaataa attaataaac
    15961 tgggatgcta taaatgctag gactatatat ccacccatat cactcatact actacccatc
    16021 atagatacta aatctttgtc actttttatc ttcttactta cttttccata cactaatcct
    16081 ggagttaaaa atataatagt gattatagga actatacctt tcattaaagg tgcattacta
    16141 gatagtatag aaccagtctc tggatctttc ataaatgcat tgtcacctat acataaggca
    16201 actatcacta ctactacaac taacaatgat aataaagcgt attttatacc cttattttca
    16261 agttcactca attcctgtat atccttatca tttgttgaat cactatattt tgtaaaacga
    16321 ggagctataa atttttcagt tacaaatgct cctgcaaaag ttaaaactat cgctgatata
    16381 aaagcaaaat aaagattcat tgctggattg ccttggtaag atgggtccat catttgagct
    16441 gctggtattg taaatccagc taacaataca tctgtagtac taagcataac atttgctgca
    16501 aatccccctg caacacctgc aaaagctgca aacataccta caagaggatg tcttccaatt
    16561 cctaaaaaca caagtgctgc aagtggtggt aatattataa atcctacatc tgctgctgta
    16621 tgtgataaca tacctgcaaa tacgattgct acagttacaa gtttttgtgg aactttactt
    16681 ataccctttt tcattaacac ttccataaat ccagatttct ctgcaacacc tgctccaagc
    16741 attgtaacca gtactaatcc taaaggagca aatgactgga agtttgtaac tatgctacct
    16801 aaatatgatt gaatctgctc tttctctagt agatttacta cctttacaac ttcttttgta
    16861 gaaggatgta tttcttctac tcctaaatta gatataactg ctgacaatat tacaacaatt
    16921 atacatagta ataaaaatat ggttactggg tctggaagtt tatttcctgc tctctctaca
    16981 acatttagtg ccctcatcaa taatccacta tctttgtttt tttcctgtga attaattttc
    17041 ctcaaactaa taccccctca taaactttat ctccagaatg ctctatctta aattttattt
    17101 tttaatttat ccatataatt tataacagac tgacatagta catttgtacc taccaatata
    17161 gaatttttat cagctttaaa tttaggatta tgccactgat atatacaatc ttgttctttg
    17221 ttgcctaccc ctaaccagta aaaaaatcct ggggcttttt ccatgaatat agaaaaatct
    17281 tctcctcctg tagaaggtat tggatcaact atagcatctt ctgaatacaa atcacacaca
    17341 gacttacaag ctattgcgta catctcttta ctatttataa cagctggtaa atcaaataaa
    17401 taatctagct ttgcagttgc ttgatatcca cgagctatct cacaaactat attttctaaa
    17461 ttttctgata ttttctttcg tacttcatta ctaaagcttc tacatgtgcc tgtcatatct
    17521 accttttctg aaattacatt atttgcagtc cctccattta tagtacctat tgatataaca
    17581 gctgattcta atggacttat gtttctgctt actatggttt gaatacccat tataattgca
    17641 cttgatgcta ctatagggtc tattgttctg tttggtatac ctccgtgacc tccaaatcca
    17701 tttacttcta tcttaatagt gtctactgct gccattaacc cacctaactt aacacctatt
    17761 tttccacaag gtatatctgg atgattatga agtccaaata tagcatcaac ttttggattt
    17821 tccattactc ctcgttccac tagcatcttc gcacctttat tgacttcttc ggctggttga
    17881 aatataaact ttacatttcc atgaagacta tctctaatat gagacaatac atatgcacat
    17941 cctacaaggc aagacgtgtg tatatcatgc ccacatgcat gcattatacc atcatatctg
    18001 gatttatact taatatcaac ctcttcatat attggcaatg catcaatatc tcctcttagt
    18061 gctactgtgg gaccatcata cttccctttt aataatccaa caactcctgt ttccattcct
    18121 aaatctaata tttctatgtc taaacttact aataaatctt ttatagcttt tgtagtccta
    18181 aattctttaa aacttaattc ggggtgcata tgaatatctg taaaaaaatc taaaatatct
    18241 tctttatact tttctgagat taaaataagt tcatgcatac taacacctct ccaaaatttt
    18301 atttttttat tttttataat ttaactacaa attatctaag ttaataatct ttataaagat
    18361 attttttata aatttaattt tagtttatat ttatatttta c
//
LOCUS       DAEORZ010000082        18236 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEORZ010000082 DAEORZ010000000
VERSION     DAEORZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18236
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.082"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(262..1083)
                     /locus_tag="KRH39_002402"
     CDS             complement(262..1083)
                     /locus_tag="KRH39_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG8512301.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            complement(1273..1626)
                     /locus_tag="KRH39_002403"
     CDS             complement(1273..1626)
                     /locus_tag="KRH39_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512302.1"
                     /translation="MKKSNIFSIIFSTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLSKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            complement(1668..3110)
                     /locus_tag="KRH39_002404"
     CDS             complement(1668..3110)
                     /locus_tag="KRH39_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG8512303.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKSNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            3786..4997
                     /locus_tag="KRH39_002405"
     CDS             3786..4997
                     /locus_tag="KRH39_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG8512304.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            5089..5604
                     /locus_tag="KRH39_002406"
     CDS             5089..5604
                     /locus_tag="KRH39_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBG8512305.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(5871..7970)
                     /locus_tag="KRH39_002407"
     CDS             complement(5871..7970)
                     /locus_tag="KRH39_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBG8512306.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8094..8633)
                     /locus_tag="KRH39_002408"
     CDS             complement(8094..8633)
                     /locus_tag="KRH39_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG8512307.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            complement(8636..9376)
                     /locus_tag="KRH39_002409"
     CDS             complement(8636..9376)
                     /locus_tag="KRH39_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG8512308.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDINTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            complement(9378..10427)
                     /gene="vorB"
                     /locus_tag="KRH39_002410"
     CDS             complement(9378..10427)
                     /gene="vorB"
                     /locus_tag="KRH39_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG8512309.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            complement(10444..10680)
                     /locus_tag="KRH39_002411"
     CDS             complement(10444..10680)
                     /locus_tag="KRH39_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8512310.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            complement(10695..11798)
                     /locus_tag="KRH39_002412"
     CDS             complement(10695..11798)
                     /locus_tag="KRH39_002412"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG8512311.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            complement(11901..13331)
                     /locus_tag="KRH39_002413"
     CDS             complement(11901..13331)
                     /locus_tag="KRH39_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8512312.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            13989..15353
                     /locus_tag="KRH39_002414"
     CDS             13989..15353
                     /locus_tag="KRH39_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG8512313.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            15377..16030
                     /locus_tag="KRH39_002415"
     CDS             15377..16030
                     /locus_tag="KRH39_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8512314.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(16149..16931)
                     /locus_tag="KRH39_002416"
     CDS             complement(16149..16931)
                     /locus_tag="KRH39_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBG8512315.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(17048..18214)
                     /locus_tag="KRH39_002417"
     CDS             complement(17048..18214)
                     /locus_tag="KRH39_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG8512316.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
BASE COUNT         6019 a         3110 c         2193 g         6914 t
ORIGIN      
        1 attataacat ataaccattt tttgttacaa tatttataaa aaaaaactat acaagaaatc
       61 taactatagt ttttacaaat aaattttaaa ctattttcct agtatactct ttatttacta
      121 aagactttga ataattatat acaattattt tttataaaat aaaaaccttt agtatttttc
      181 tagaggatac aatatcatga tattaatttt tatactaact tttaaattat ttaaaaaaac
      241 tatatatgct atattagctc tctatgctat attattagat ttaacttttt ctatatttac
      301 aaaataagtt tctctttctt taaataaaga tacatatttg aagtcaagat acttaagcat
      361 atcataggcc acatcaaaat ccttacatat atcttctacc ctatgagaat cagaacctac
      421 agtaactata aatcctccaa gttctttata tctttttaga atatcttcat ttggatgtat
      481 agcatttagt ccatatctat aaccagaagt atttagctct ataccactcc cattattgat
      541 tattgacttt aagacctcat caataacatc tttaaagtca ttgtatataa ctctattatc
      601 tgaatatatt ccatacctag atatataatc caaatgacca tatatattgt aatctccaaa
      661 ttcacgatta gtttcaagta tgctattaaa atatttataa tacgcatctt ctttgctaag
      721 tcctttaaaa tacgcctttg atggaacagg tacattgtca attgtatgag ttgaacacaa
      781 tacaaaatca aaaggattag aatttattat ttcttctatt tcatttttta gatgttttct
      841 ataacccact tcaacaccta ataataactc tatgtccttt ttatattttt ctttaaactt
      901 attaaatact ggaacatagt cattataatt tatgcaaaat cctctatctt catcatgttc
      961 taaatggtct gtaaaagtta aatatctaac accaatactt atagcttttt ttattgtatc
     1021 ttccatggaa tcctttccat cacttgaaaa attagtatga atatgattat ctattaaatt
     1081 catattatcc ctcccttatt ttatctatct taataataca acaagtttat tgtatacatt
     1141 tgttaaaaga aaattataat ggttaaattt tatttacata cttttatttt tacaaaaaag
     1201 agaagcacct atttctgatt ttagaaaatc attaaagata cttctcttaa tgttactttt
     1261 atatacttca atttaatttt taaaagtctt aattttgtaa tagagtttag ctgttatatg
     1321 tccaatcata taaaaagctg tataaagtaa aacatatata tatgcagagt catttaaaaa
     1381 tatatacatt aatactatat aagttactaa agacacaata agagataaaa tccaattagt
     1441 cttacttaat acacaaatta ttccttgtat caaaaaagaa attggaaaaa tcaaaaccat
     1501 tcccacaaca aaaatacttt tagattgaat atctggaata ttaaatatat ctgtagcaaa
     1561 agttaaaaac attagcacca ttatgactgt agaaaatatg atactaaaaa tattactctt
     1621 tttcataata atcaatcctt atttattgga tatttgataa tcttcaatta ttttgttgcc
     1681 tcaatttctc ccatcattct tactaaatct tgtccatatt gacctccagc ccaattatta
     1741 gatagacttt caactgtttt agccttacca aataaagatt tttcatgtct agggtctact
     1801 ggatttgaaa gtgggtaacc atcttgtcct gcatataagc ataaatgttg aatttgagct
     1861 aatattcctt cttcccaagt atcaaatctt gaatgggcat tcttatcagt atcagaacca
     1921 cctacagaag ttttaagtcc acaaggattc ttaaatgatg catctaatac tccaccaaaa
     1981 ttacaaaaac cagtttcttt tgcagattga gctactgcca aaacaggatt aacaccttcc
     2041 tgaacagcag tatcatataa tattggtatt aactctataa ataaatcatt agctttttta
     2101 gatttggccc attttaagca cgcttctaaa ctagctgttg gctgacctaa tatgcttgtc
     2161 tcatttttca taggtgactc accttttgta gacgaatcgc cattattttg tgcagcagaa
     2221 acacacttat tatacacact gtcacttatt gaacctaatc tagtaatctt atctgctcct
     2281 gctaatatag acttatcact actttctgac accaatacaa ttggattttc cttagcaatt
     2341 gtagaacctg taagtgcatt tactaaatca tatccatcac ttaaataaaa ttctgatgca
     2401 tctggataaa actcttttat aactttctta tttgtatcat atctatctga accaccaagt
     2461 cttttagcat tacttttatt aactaaacta gtgctcatgg atatacttcc acctactgca
     2521 tatgttttta cattacttgt tgaaaaaggc acactctttc catctgtaag tactatagga
     2581 gctatatctc ttactgatac gggtgcagca cttattgtat ctgcttctcc tttatatgca
     2641 ttagttaata tcacttcatc aacttttttg ataccattta cctctttagc aacactataa
     2701 ctagtcttta ttctgtcatc tccacccaat ctagttacct gtgcccctgt gtttttcagt
     2761 tcagtttcta ctgacttact tattgataat tctttaccta ttatgtatat ctttttaaca
     2821 ttttttaatc tttgttttgt ttcatttggt atcttatttt tttgtgcaag caaaattgga
     2881 gcatcaacag cacctgcaag accacttgct gaaagaccat ctgctataga attatccata
     2941 tttaccataa ttgctgtatc ataactcttt ttatctgcta ttaatccagc cgtctcatat
     3001 atattgttac ctttaattgt atctgatgtt aatgcatcta cattaggtac atttattaga
     3061 aatatagcta atgttaaacc taatgctaaa actcttctaa ttgttctcat attcttcccc
     3121 cttgatatat caagtttaat tcaaaaatga ctataacata gtagtacatc tacgctatag
     3181 tcatttatta taatttaata attatatatt tgttattata tcataaatta caaaaagttt
     3241 ccataatatt gtatagatta aaggaaaaat ttacatttta gttacaattt actagatttt
     3301 tgttctaatg tatctatatt tagtaacaat ttgtaataaa aatcgatatc tatatttcat
     3361 atttaattca ctgttttaca aaatgaaatt ttattgtata caataaacag attatatgtt
     3421 tacataatct atattatttt taagtaaatc ttttatcgat attattaaga atatatatta
     3481 catcaagttt attatgtgca aaccaccatc aacttaaata cctaaggtaa cacttgaagt
     3541 agtagattct tggtaaaaat aattattgct atcattatct ttttttaatt aagtatttac
     3601 tatttatata tcaatctact tttcactttt aatttaattc taacattttt ttatatgttt
     3661 tatgatatat aatgtgatat attctaatta atgatatttt caagtaaaaa aatgttactc
     3721 tattataagg tgatagtata cttgaaaata aataaaacac ttaactttat ttaaggagat
     3781 ttacgatgag taacaaacaa ggttctaaca tcaaagatgt aaaaattaaa aataagttaa
     3841 taatattgaa attactttct acaaatacac ctatgtccag agttgattta gctaagtcta
     3901 caggacttac taaaatgact ttatcaaatc tagttactga acttattaat gaaaacatga
     3961 ttagggaaat agagtcatca actttaaatg cacattcatc aggtcgtaaa cctattttat
     4021 tagatatttc taatatatcg ccttgtattt gtggaatcct aataagaaga aatatatgta
     4081 aggtaattat ttcagattta aaaggtaata ttgtaaaaca actttctgaa acttacccta
     4141 aatgtctatc aaatgatgtt ttaaaagata taataaaaag aatgtttact aaaataatga
     4201 aaatcaacaa gagagatata attggaattg gtatttcttc aataggtcca gtagatgata
     4261 taaatggtac catactaaat cccccagact ttggaaatgt atcaaaccta aatatagtag
     4321 attttataaa agaaatgtct gatttgccaa tatttttgat aaatgatgct aattcgggtg
     4381 cattagcaga aaaaatgtat ggtttaggta aaaatatatc aaattatata tacctacaca
     4441 taatgaatgg tattggagct ggtctagttc tagaaaataa acttcatact ggcaacctag
     4501 gacaaagtgg agaaatagga catacatcaa ttaattttag tggaccattg tgcagttgtg
     4561 gtaacaatgg atgtttagat tattatacaa gtgtcagtaa tctaattaaa agaattgaat
     4621 cattgagcca tatttatcct aactccccat taattaactg taaagatttt tctttagtca
     4681 agttaataga tgaggcaaat aataaagata gtcttgccat gtttttgctt gatgaatttt
     4741 gtacatacgt atcatatgca ttagttaata cacttacatt gattgattgt agttctataa
     4801 taataggcta tgactttaat atacctggta catttataga aaatacatta ttaaaaaaat
     4861 tgactgcttc agcaagtttt tctaaatata aaaaaataag tgtaaggcat tcaaattttg
     4921 gagcaaatgc accattaatt ggctctatag ccattgtaag ttataatttt tttaatggta
     4981 gtataaattt ttattaaata tataaaatat ttaaacactt ttattgaata tttttccatt
     5041 taatattatt atattttata agagcattta aaatttggag gtataaaaat gatttcagct
     5101 aaaatggaaa aattacttaa tgagcagatt aaccatgaat tatattctgc gtatctttat
     5161 ctttctatgt cttcttacct tgaagcagaa ggacttaaag gattttctaa ttggttttat
     5221 gttcaatata aagaagagac agaccacgct atgttttttt acaaatactt acataatgta
     5281 ggaggaaagg ttcagcttga tgctatccca atgccagatt cagactttac ttctgcaatg
     5341 gatattcttg agcgtacgtt agctcatgaa aaaaaggtta cagctttaat aaacaactta
     5401 gcagctgtag caaactctga atcagacttt agaacttctc aattcttact ttggtttata
     5461 tctgaacaag cagaagaaga aacaaattgt gaagacaata taaaacgtgt taaacttgct
     5521 ggagagggtg gtctattctt tgttgaccaa gaatttgcta atcgtacata tgcagctcca
     5581 acaaacccac ctgtgacaat ataacgttta aaaattttta atataataaa gaaggtgttt
     5641 taatgaaaat caagattgat tcttatcatt aaaacacctt ttattattcg ttttatacgt
     5701 agtattttat tgtttataca gtgctatttc acaatgaaac ttaaaactat actttcattt
     5761 taaaatattt ttaaattaaa tttggggtga ctgaaaatca cactatctaa cttacaaaat
     5821 gtgattttca gtcggtgctt taattatggt tgaataccaa aacttataaa ctatttatat
     5881 ttaactatta aggcatctac tgcacatacg ccttttcctt taggatatac aaacactgga
     5941 tttaagtcta tttccttgac ttcatcctta ttttcataag catattttcc taatttgacc
     6001 atcatatctg caagagcatc aatatcgcaa ggctcacttc ctctatagcc atttaaaagc
     6061 ttaaatgatt ttagtttttt aagcatcata atagcttcat catgatttat aggtaatgga
     6121 tataatgtag tatctttaaa tacctctaca aatactcctc ccaaaccgac taataacatt
     6181 ggtccaaatt ggtcatcatt agttatacca ataataattt ctataccact ttctaccatt
     6241 tcttgaacta atataccatc aatattagca tctggttttg ctttttttac attagttaaa
     6301 atctcattat atgcatctac tgcctcatct ctattcttaa ttcctatttt aactcctcca
     6361 acatcagatt tgtgcaatat ttcagaagaa tttattttta aaacaacagg atacttaata
     6421 tattttaaga tttcatctaa ttcatcaata ctttttgcat taccttgtcc tggaactggt
     6481 actccatatt ttttcatttc aatcttacta tctaattctg ataatgctac tacattattt
     6541 gactctcttt tcttaacagc tttaaattct aaagttcttt ttgaataatc ataatctata
     6601 aacttagcca ttttattaaa acaagtaaat gtagttccaa ctgatgacat tataggcact
     6661 tgattatctt ctaatctagt cctataatct aaatatctat ttccttcaag tgtaggaact
     6721 gcaaacacag gttttttcaa tccttgttct ctagcttcac ttacagcttt acaaagggtt
     6781 tcatgcattt cattttcttc atctcttata tttgcaccta ccatagtaaa tccaacacta
     6841 tcatcctttt caaaggtatg taataaacct actatcatat ccttttcatg gaataatgct
     6901 gttgtagcat ctagtggatt tttaggtgtt gcaaatcctg gaagatattt tttcatttct
     6961 tctttagttt cagaagatat ttctgcaagt tctacaccat tttcttctgc taaatctgca
     7021 caaatagtat tttctcctcc tgagaagttt attgctgcaa aattagaatt tgcaggtaaa
     7081 ttaccatcta aaacactaaa agcttgtgct aaacacataa actgttctaa attttccaca
     7141 gaaattactc catatttttc aaaaatactt tcaaaagctt tactcgaacc agctaaattt
     7201 cctgtatgag aagcagcaga aatagcacct ttttctgacc ttcctgattt caatattatt
     7261 actggctttc ttttttgtgc tgcccttttt aaagcatcta taaatttttg aggatttttt
     7321 aaaccttcaa ggtaaattgc tattactgat gcataatcat catctacaag gaaatctact
     7381 gcatcttcta gtgtaactat atttccattt cctgttgaaa aaccataaga tatattaaaa
     7441 taatcacttg aaacaatttc agcagttata aatccacttt gacctacaat tcctatacca
     7501 ggttttttag tatctaaatc ccatttagaa cctcctgccc atagattaac cttatctata
     7561 caatttagca accccatgca gtttggaccc aatattttca tatcgtatgt atttgatatt
     7621 tctataagtt gattttctaa atctgtacct tcctctgtac cttcctctgc aaatccacta
     7681 gcatatacca ctgctgcctt tgttcctaac tctccagaat cttttaatac agatggaact
     7741 acatttctag gcgtacatac tactacacag tctactactt ctggaacttc ttgtatcgtc
     7801 ttataacatt ttctcccaaa caattcttct cttttgggat taacataata tactctatct
     7861 gtttccttac tttttactat actaagtgct gcacttcttc caaaaccaag tttatcagtc
     7921 actcctacta tagcaatact ttttggtcta ataagttttt ccaaactcat aaattcacgc
     7981 tcctatatgt ttatagttgt gattttaagt gaattttatc tcaagatgaa gtttctcttg
     8041 gcactaaaga ttattaaatc ttgagataaa tcacttaaga taagggtaat ttatcaaatg
     8101 gcgttattat agccttccat gaatgctgct tcattttgaa cattaaactt tccttttcct
     8161 ttttcctcaa agaaatcaca catagttttt aaagctaatt ctttttcaaa taacccagtt
     8221 ttagcaatta atgctccaag tactactata tttgctgctt ttggattgtt tactttttga
     8281 gctaattcct gacaatttat tttaactaca cttatatctt ctcttaatgt tacattctca
     8341 tctattgcag atgtattaat aactactgtt ccaccttctt taactaaatt tatataatct
     8401 aaagatggtt tattcattgc tactaaaaca tctatttctt ccatatcagg cccacctata
     8461 ctctcatcag aaaactttac tacactatag gcttttccac ctctcatagt tggaccatat
     8521 gcaggcatcc aagttacttg tttccccaat tcaagtgctg actcagctac aatcagacca
     8581 agagttaaaa ctccttgacc accaaaccct gcaaatacta gctctttttc cataactatt
     8641 caccatccct ttgcttaaat tcgccaagag cataatatgg cactatttct tcttccatcc
     8701 atttgataga tttctctagt gatacacccc agttagttgg gcaaggtgct aatatttcaa
     8761 ctaaggaata tccctctcca tttaattgag cttctattgc attttttata tattttttta
     8821 gattaattat ttcctttggg ctatgaactg aacctcttgc tacatacgct acatcaaaga
     8881 agtttgccac catctcagga actttcagtg gactaccagt agtatttata tctcttccat
     8941 ttacacttgt tgttgtgact tgacccggca tagtggtcca tgacatttgt ccaccagtca
     9001 ttgcaaaatt attattgttt attacaaata cagtcacatt atgatttcta tatgctgtat
     9061 ttaaagtttc agaaagacct ataacataag catctccatc accttggtaa gtcattacta
     9121 atgtatctgg aagtgctact ttaactccta tagctgtaga tgaagctcta ccatgtgcag
     9181 tttgcatctt atcaccattc cagctaaaat tcatattgca gacacaacca actcctaatg
     9241 ttaatacatg atttttttca tatccatgtt cttctattac ctctgctatt atacgattta
     9301 ctattccatg accacaacct gggcaaaact tgtttggtat tgaaacaacg ctaggtgtta
     9361 atttttctgt agccatatta gaacacctcc ttgatttcgc cagattttat tttgctataa
     9421 gtgtccatta cttgcaatgt ttttggtata tgatttccac taaataaacc atacacaggt
     9481 acattttctt tacatacttt ctttgaggct aaagcaacat cttctactaa ttgacctgtg
     9541 tctgttgatt ctactgagat aaatcctttt acatctttac ttaccttttc aaatgcttta
     9601 tatgggaaag gccatactgt tattggtctt attattccaa ctttttcacc ttgttctcta
     9661 agtcttctaa cagcattcat agttgttctt gatggtatac cgaacgctac aaacacatat
     9721 tctgcatctt ctacttgata atcttcccat tgttgttcat tttcagttat tctcatacgt
     9781 ttctccatac aaaaagttgg cttttggtta tctgcaacct ttgcatggtc tctattactt
     9841 ccatcataag tccaacctaa atctccttct ctcttcttaa actctggaaa ttctactggt
     9901 tccatcattt gacctaaagc tgcttctgtc aaaatttcga caggcactct gtacttctcc
     9961 gctacatcaa atgagttata catcaaatct acagtctctt gaatactgtt tggtgcataa
    10021 actatcaaat gataatctcc atttcctccc cctctagtat ctcttagata gtctgtctgt
    10081 gctgagtcca atgagccaag accaggcccc catctctgta catttacgat tacacaaggg
    10141 taatgatttg cacataagta agaaatccct tcttgtttta aagatattcc tggtcctgaa
    10201 gaactcgtca tagacctcat tccacttgca tatgctccat ataccatata tatagcagcc
    10261 atctcatttt cggactgtat aaaagttctt ccaagttcag acattctatg tgataaaaat
    10321 tccattacct ctgtttgtgg tgtaattgga tatcctgcat aaaattcaca acctcctctt
    10381 agggctgctt ctgcaatggc gtggttcccc ttccacatct ttttcatatt atcccaactc
    10441 cttctaagcc tcttgacatg cttcttcttt aattatttca aaaacattat ctggacacac
    10501 ctgatagcaa attccacaaa gtatgcattt actctcatca acttctacat gagcataacc
    10561 ttctttattt atcttacttg atatttttaa tgctcctttt ttacaagata taacgcagta
    10621 ttcacaactt ttacatctct ctgcgtttat ttttaagttc atatctacac cccgtttcat
    10681 aatatttcta tctattatcc aatgcttggc tctcctttaa tatttaatat ttcctttaaa
    10741 actgctacaa atttcttatt ttcttccata gttccaatac ttacacgtgc atgtgtagca
    10801 cttggtctta ttacaaaccc tctttttttc aagtcttcaa ataacttagg tatatctgtt
    10861 ttcatatcta cataaaggaa atttgattgt gaatctacaa catcaaatcc catttttgta
    10921 aactcttcct taaagtaagc cattccaact ctatttaatt catatgtttc ttttataaac
    10981 tcttcatcat caagtgctgc tgttgctcca cttatagcaa cacgatttgc agagaaaggc
    11041 tcttttactg taaataaagt tcttattatc tcatctttag caataccata tcctactcta
    11101 agccctgcaa gaccatatat tttagagaaa gttcttgtta ctataacgtt ataattttcc
    11161 cttacatact taagtccatc cacataatta ggaactgtta aaaattctat atatgcttca
    11221 tccaatatag ttataatatt ttcagggact ttctttaaaa attcttctaa ctctttatca
    11281 tccacaattg ttcctgttgg attattaggg ttacatacac atattaattt tgttttatct
    11341 gtaatcttat ctaatatacc ttgtaaatca tacttataat cttctgttaa aggtacttct
    11401 actggtatcc cttcattttt tatagttgca gttctatatg atggaaatgt tgggttacaa
    11461 tatataactt catctcctct atttataaaa gcctcaccta ttagagtgat aacgttatct
    11521 gcaccattag caacaataaa attttctggt tttaaatcta acttttcagc taattttctt
    11581 ctaagttcgt tagcttctgg ctctggataa agttgacctt gttttaacat ttcaatcata
    11641 gcttctattg cttttggtga tggtcctaga ggattttcat ttgaagctaa ttttattatt
    11701 tcttttacac catatttttc cttaactttt tctattggtt cacctggaac atattgatgt
    11761 aattctttta cggtatttct caataatttt tctatcattt ttaaaagccc cctctgtata
    11821 tgtattttca tttatataaa cgctattgcg tctacttact ttaatagctt aaattaacca
    11881 tctacttaac tttcttttaa ctaagcattt ctttgattta actcttctct tatttggttt
    11941 aatttcttgt cacttactgg atatagtatt gctataacaa cagctattat taaaggtatt
    12001 gccatagaat aagaaatcat atttataaaa cctgatttaa ctgcttctgt gactaccata
    12061 tcagcttgat atcccattga gttcattaaa actgttctta tagtagttgc aagtaatact
    12121 cctacttgta ttgcaactgg atatagtgac atacttaagc caggtaagtc ttttcctgat
    12181 ttccaatgcc cataatcaca agaaccggca aagaaattaa gatttattat tgatactaca
    12241 tttgaaaaac ctccaccaat tgccagcaac atagttactc ctaaagtagt tttcccccaa
    12301 ggagtccatg caaccaataa gtggcatagt gcacatatag aaaaagaaat tattgaagct
    12361 agtttttgct ctctacagat ttttataaac actggtgcga aaaacatgaa tgctacactt
    12421 accaaaggca tgattgataa aaatactgct aatccacgtt catcttctat tacatattta
    12481 aaataatatg gagccaaagg tcctaatcct atagatctta tacatataag aaattctaaa
    12541 caaaatgcca tcataagtgg tacattagtg aataaagctt ttgccatttc acttgcttta
    12601 atctttggct tctcttcttt ttgaagaact tcttcttctg ctccagattt tattatctct
    12661 gttgaagaaa accaaaatac taaaaggcat cctgctgcaa agaataagta ggtgcctaaa
    12721 taacccattc cttctccacc taatacatct gcaaataaaa ctaataataa cggatatgaa
    12781 aaacctacta agaatttacc aacttcttta aatatattcg tagtagttga gcctataact
    12841 cggtctctag gtgtcttagc aactacaggg taaatagcat aaattatagt gaaaactact
    12901 tgctgagcac aagatgctat taataataaa gctgtcatca aaggtaaaag taatgctgga
    12961 cttattttca ttaatggaac tatgaaaaat attccatatg ttaatgctaa cgctgctggt
    13021 gcatatctaa gccatggata atactttcct tttttgaatg gttgtttgtc aaatactgta
    13081 cctgttatag gagctacagc aatttttatg aaagattgta taatttgcat accacctgcc
    13141 ataattacac caagttttgc cacatcagtt aaaaaaatat ttgtaacagt tgatacaaag
    13201 taacctataa aattgtacgc taagtttata gaacttgaat atgtgtatat tgctcttttc
    13261 ttagggtctc tcatatcatc tctaaaattt gatataagat ttccttttgg tttgctttct
    13321 tgactactca catctacccc tcccttataa aattttctta ctttttatta ttcacgtata
    13381 ataatatata ttttgaatct taaatatctc attcttgatt caggagtata tttttatatg
    13441 tgtataattt taatttaatt atattctcaa aattcataat tgtcaaacac tttttaagtt
    13501 ttatttttta ctactaaaat taactaaaaa gctatttaga cttaatttag gtttgttttt
    13561 tattattttc ttgttttgaa atgtcattga aatgcgtatt ttaaattatt ttatcatatt
    13621 tctagtgatt ttaatttttt ataattttat tcaagaataa tttattcaag atatttctat
    13681 actacatcca gatattaaaa atatcttttt cttatttaaa ttccttttat ttttatacat
    13741 gtactattga tttaaaaata aaagcgtgtt acaattacaa aaaagaatct atgagataaa
    13801 tatcaatatg caaaaatttc agaattttca attttctatt tttacgtaat ttccatctaa
    13861 tatcataaat tattatagct atattaatct aaaaccaata aaacaaatat cgtactactt
    13921 ttatataaaa tatattttaa atatattttg tatattttat taaaatattt tataaaggag
    13981 aaaagactat gttactttta attgttacgt gtgcaatata catatggatt ggtttttatt
    14041 tttcaaaaaa agttaaaaca gcatccgatt tcctaatcgc aggtagaaat ctgcctttgc
    14101 ctatactagc tgcaaccatt gctgctacat cttttggagg agggtgctta gttggtggag
    14161 ttcaatgggg agctgaaaga ggactttggg ttggaatgta ttctactata ggtgcagctt
    14221 tagcttgctt tataaatgca tttttcgcag gaaaattaag aagcttatct tgtgatataa
    14281 cacctgctga ttatattgaa actagatatg gtcatagtgt atttttaaga gcttatcaaa
    14341 gtcttgtaac accaatatct atactagcta ttatgggtag tcaattgatt tcttttggtt
    14401 ctgtatctac tgcatttggt attccttatg atatagctgt aattatagga gttatagtta
    14461 tcattatata tacttataca tcaggaatgt ggggagttgc agtatcagca tttattcaac
    14521 ttgctatttg tatagtgttt ttgccaatag tagcttatat aagcttaaaa ttagttggcg
    14581 acaacccttt acttatgtta caatcaatgg taaaagaacc attctttcca ggaaataaat
    14641 cgattagtga ttttatgtat acagttttac cattagtttt aggctcaata ttttcttatg
    14701 aaagtttttt aagatatcaa tgtgctgata atgctaaaaa tgcagttaaa agtagtgtaa
    14761 ttgcaggatt tatactatta tttcttgctt tccctatagg aataattgga gctattggtg
    14821 gtagtttatt tccaaatata tcatctgttc aggttttacc tcaaatgata tcgactactt
    14881 tgccaccttt aatatctgta ttattcttag ctgctatatt ggctgccata atgtctactg
    14941 ccgattcatt aatgacctca atgagtgcta ttatatcaag agatatttat aacaaattat
    15001 tacatcctaa tatagaattt aatgatttaa aaaatactct aaagtattct caaattgcat
    15061 cagctttgac agctataata ggtgcaatca tagcacttaa atttgatagt ttattagaat
    15121 tattgttttg gccagctcca ttatgtacag gtgtaatatt tgcaccattt gtaattggtc
    15181 tttgctggaa aggtgctaca aaaaaaggag ctatctatgc tgttatagta agtgttatat
    15241 tggcattatt aaatatgatt ggtattctta ctgtatttga cagaattttg gttcctataa
    15301 taggtggttc tattacaata tttatagtta gcaaattttc aaaaaaagat tgaaaagaga
    15361 ggtatacaca gtaaatatgt caaaatcaga aaagggcaaa gaaacaaaag ctagtattat
    15421 aagtgctgct agagaattat tttttgagca aggttatcat aagacaacaa ctaggcaaat
    15481 tgcagaaaga gctaatataa atttaggtct aataagctat tacttctcaa gtaaaagcga
    15541 aataggagct ataatatatg aagatataag aaatgaaatg cagtctttaa tctataacta
    15601 ccatgaagaa ggtactatga tgatgttctt tcttgctatg aatgctgacc tttgtttcct
    15661 tctaaaaaac gaagcatata gaaattttta tttggatata gcttatgaag aagctatttt
    15721 aagctatcaa agaagtgtaa ccgaaatggt catgaaaaga tatgttaatg ataaagaatc
    15781 tatagaacat gatgaattaa tactttcttg tattggtata cttgctatga aacctgaaat
    15841 aattagagtt tatacttcac acaagtacga tatatcttct caagctgtaa taaagcattt
    15901 tacaaaacag tttcttatta atcttggtca tagtacggat ttatgcgaag agattttaga
    15961 ggaattgagt aaatactata tagatatagt tgacaatttt actccaataa taactcgtgt
    16021 aagaacttaa taaaatttta ttaaattctt agagactgaa attttaaaat cataatttga
    16081 ttttaggatt tcagtcttta tcatatttaa agatatttta tagtatacct gttaatacca
    16141 taagtgtact atccaattat atctataaat ttctttatcc ataaattcca aagacgccat
    16201 tcatgccccc ctacacactc ttcatattca acatctacac ccagagaatc tgcatatttt
    16261 ttaaacatct gattattttt atataaaaaa tctctatcac cacaaaattg ataaatttta
    16321 ggaatatctt catttctttc tttaaggttc ttaagtatgt aaagtaaatc attctcacta
    16381 tttctagcgt cttctggtct accaaaatcg tgaaatagaa tgtctgcatt ggtttctatt
    16441 tgttgatttt ctaactcatg aatcatatca ggcgaaccag aaaaacttgc aactgccgaa
    16501 aactcttttg gttttgaaag cgctaatttt aaagctccat atccccccat agacatacca
    16561 gctacaaagt tatcttccct tttatctgat aaaggaaata tagctctaac aaatttaggt
    16621 aattcctcgc ttacaaaagt aaaatattgg tgtccatacg ccatatcggt ataaaaacta
    16681 gtttctactc ttggtattac tagggctata tttttatttt tagcataatg ctctacatta
    16741 gtatatctaa tccatgagct ttggtcatct tctaaaccat gaaataagta taatgtttta
    16801 aatttacctt caaaatcata tatctcttta ccactaaaaa gttcttttcc atttgctaca
    16861 ggcaaaatta caattatatc agtttctcct ccagtatatt tagatttaaa tttacattct
    16921 ataaatgcca caaaaaacac ctcttctata ataattttat atctaaaaag caccaagagt
    16981 ttaactttct cagtgcttta aatattgctt aatttatata tttttaatta tacataatat
    17041 tttttagcta catataatcg gaagtatctg tcggtaatcc ctttttgtcc aatttacttt
    17101 caacaatatc cttaattata gaataaatta ctgcaaacat tggaactcct ataaccattc
    17161 caataaaacc taataatttt cctgcaacca ataaggagaa caaaatccaa aatgcagata
    17221 taccaattga atctccaagt attttaggtc ctataatgtt tccatctatc tgttgtatta
    17281 ttaatataat taacaataac cagaaagctt ttataggaga tacaaacatc actattagcg
    17341 tggatggtat agctccaaat aaaggtccaa agaaagggat aatatttgta ataccaatta
    17401 taactgatat taataatgta tatggcattt taactaatgt aagtattaca aatgttagaa
    17461 tacctataat gaatgaatct aatattttgc cacctaaaaa tcttccaaat gtatagttac
    17521 ttctatgagt taattctaaa attctagcag cttgtttttc tgtaaatact gcataagtta
    17581 tttttttgct taatccatag aatttttctt tatcaattaa taagtatact gaaacaatta
    17641 atcctagtac aatattccat atactagagg ctacattctt taacatactt cctaatattg
    17701 ggattaaatc tgttacaaac tttataatat atgtaacaaa ctcattccac ttatcaactg
    17761 ccaaattaaa gtattgctca tcaagattta aatcagtcat taatttatca ataagcttag
    17821 ttgcattgtt tacatacata ggtatatcat ttgctagtcc aacaatactc tcaattactt
    17881 gtggcaatac gaaatgcata aataagtaaa gtatcaatgt agctgttgca tatgttaata
    17941 aaagacccaa gcctcttttt gaactttgtt ttagtttttt taatttctca ctttttataa
    18001 aaattctgtc ttcataaaac tttaaaataa aattaagaag atatgcaata gcaaatccta
    18061 ttataaaagg ttgtaatgtt gtaaatatag catctaaatt attagaaaat acatcaattt
    18121 gtgatgatac tagataaaat ataatactac tagcagctat tataaatgta taaattgcta
    18181 ttgttgtgta ttttgtattc caattaattt tcattttttc ctccttattt ctatat
//
LOCUS       DAEORZ010000083        17848 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEORZ010000083 DAEORZ010000000
VERSION     DAEORZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17848
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.083"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            169..1542
                     /locus_tag="KRH39_002418"
     CDS             169..1542
                     /locus_tag="KRH39_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG8512317.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1798..2865
                     /locus_tag="KRH39_002419"
     CDS             1798..2865
                     /locus_tag="KRH39_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBG8512318.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     NVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            3009..3317
                     /locus_tag="KRH39_002420"
     CDS             3009..3317
                     /locus_tag="KRH39_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBG8512319.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3310..4740
                     /locus_tag="KRH39_002421"
     CDS             3310..4740
                     /locus_tag="KRH39_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBG8512320.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4818..5192
                     /locus_tag="KRH39_002422"
     CDS             4818..5192
                     /locus_tag="KRH39_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBG8512321.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5186..7390
                     /locus_tag="KRH39_002423"
     CDS             5186..7390
                     /locus_tag="KRH39_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG8512322.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7390..8799
                     /locus_tag="KRH39_002424"
     CDS             7390..8799
                     /locus_tag="KRH39_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBG8512323.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHNINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIKYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVC
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8784..9848
                     /locus_tag="KRH39_002425"
     CDS             8784..9848
                     /locus_tag="KRH39_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBG8512324.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVAKKIIV"
     gene            9878..11299
                     /gene="nhaC"
                     /locus_tag="KRH39_002426"
     CDS             9878..11299
                     /gene="nhaC"
                     /locus_tag="KRH39_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG8512325.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKISRIEDEPETCLNFDV"
     gene            11563..12027
                     /locus_tag="KRH39_002427"
     CDS             11563..12027
                     /locus_tag="KRH39_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG8512326.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLVKLDVEVGFPVATYIDGKNEILQKIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12261..12623
                     /locus_tag="KRH39_002428"
     CDS             12261..12623
                     /locus_tag="KRH39_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG8512327.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            12691..13305
                     /locus_tag="KRH39_002429"
     CDS             12691..13305
                     /locus_tag="KRH39_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG8512328.1"
                     /translation="MNEKWLEWAIELQSIAQAGITYGKDIYDIERYERIREISAEMIA
                     YKTDISTEKVRNLFCNESGYQTPKLDTRAVIFENGKILLVKENTGKWSLPGGWVEVNL
                     SVKENTIKEVKEEAGLDITADRIIAIQDRAKHNLPIYAYGVCKIFVLCTVIGGTFKEN
                     IETTEFSYFSENELPELATEKNTEEQIKMCFEACRTDTWTVIFD"
     gene            13325..13949
                     /locus_tag="KRH39_002430"
                     /pseudo
     CDS             13325..13949
                     /locus_tag="KRH39_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860815.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            14560..15396
                     /locus_tag="KRH39_002431"
     CDS             14560..15396
                     /locus_tag="KRH39_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8512329.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            15480..17594
                     /locus_tag="KRH39_002432"
     CDS             15480..17594
                     /locus_tag="KRH39_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBG8512330.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
BASE COUNT         6959 a         2123 c         3385 g         5381 t
ORIGIN      
        1 aaataaaatt ttaaatatac ttaaaaatat attaatataa tgtacataaa aaatatcatc
       61 aatatatgat aagataaata caatagcaac attttattaa ttttttgggt ttttatatat
      121 ttaggtactg gggtgatata gttgaaccaa gagatatttt ttaaaataat gaatgaaata
      181 ttggaaaata ttgatgaagg ggtacatttt gttaataaag acgggaaaac tataatctac
      241 aataattctg tacaaaaaat ggaaaaaatg aacagacacg atattttaac caattcattt
      301 tttgatatag tcaacaaaat gaaaataaac aggagtacac tattagaagt tttggaaaaa
      361 agggaagtta tcaaagacaa tattcaaaaa tatctggata aaaatggaaa agagataaca
      421 gcaattaata ctacaatacc aattgatatt aaaggcgagt ttgtaggggc acttgaaata
      481 tcaaagaata tgacaacgat agaaaattta ctaaacagaa attataaaaa aaatttagtc
      541 aataaagtta gtagtagaca aaaaaagggt tatgaatttg atgatatttt aggggaaagt
      601 aatttgatta aagaagcaat agctaaatct aagaaagctt gcaagtctga tgcttcagta
      661 tttatatacg gtgaaactgg aacgggtaaa gagttattta gccagtccat tcactatgga
      721 agtaatagga gaaactatcc atttatagca caaaattgtg cagctttgcc ggaatcacta
      781 ttcgaaggta ttctttttgg aactagcaag ggaggattta caggagcaat agataggcca
      841 ggcttatttg aacaagctaa caaagggaca ttgttattgg atgagataaa ttccatgcct
      901 attatgttac aggctaaact attaagggtt ttacaagaag gttatgtaag aagggttgga
      961 ggaactaagg atatacctat agatgtaaga attattgcaa cgacaaatga aagtccaaag
     1021 gtaattttaa acgagcataa aatgcgaaaa gatttatatt ataggcttaa cataattcat
     1081 ataaaaattc ctcctcttag agatagaaaa gaggacatac ctctattgtg caaaaaattt
     1141 atttcaaagt ataataaaaa gttaaataaa aaagtggtag gaatttcaga tgaagctatt
     1201 aaaattttaa aaaattacga ttggcaaggt aacataagag agctagaaaa cataatttat
     1261 tgtactatga gtatgatgga agatgaggta caaatagaaa caagtatgat taatctaaac
     1321 gactattaca atcttaaaga gaataaagag agtaacaatt attctttaaa cgatttggag
     1381 gacaaaacac ttagtaagat tatcagtgag atagaagaaa agtatatata tgaatcatta
     1441 gaaaaggcat catacaatat aactaaagct gcatcaattt taggtatgaa taggcaaaat
     1501 ttacagtata aaataaaaaa atataatatc aaaatattat aaataagcaa gataaaaaaa
     1561 caaaatcaaa gtaaaataaa aaagcaaaat agaggagcaa aattattata aaagagcaaa
     1621 atattttgct ctttttttta tttctcataa gaaatattat tttttattta tgaaaataca
     1681 taataaaata attatagttt caacgtttca ataaatattt actttaattt taaagttggc
     1741 acgatttatg ctttaactaa ttattgagaa tatataaatt tttaggaggg tgtatatatg
     1801 agaaaagtaa gagtaggaat atggggattt ggagctatgg gaataggtat ggctaatatg
     1861 attctaaaga aagaaggtat agaaatagta tctgtttgta gtagaagtac ttcaggaaaa
     1921 agtatgtata atgttttagg aattgaaaga ggagaaagac cagaagttat tatcaataaa
     1981 aattatgaag aagtttttag agaaaaaagt gtagatgttg ttttgttagc aactgattca
     2041 tttactaaaa aggcttttga taaaataatt ttcttattaa atagaaaaat aaatgttata
     2101 tcaacagcag aacaaatggc atatccacaa gctgatgatg cagatttagc taaaaaaatg
     2161 gatgaagttg caaaggaaaa tggagtaagt atacttggaa caggaataaa tccaggattt
     2221 gtattagatt tactagtatt ggcgctttca ggcacttgtg aagaagttac tagtataaaa
     2281 gctaagagag taaatgattt atctccattt ggaaagtctg ttatggtgga acaaggtgtt
     2341 ggagtaacaa gggaagaatt tataaaaggt gtagaagata agactatagc aggtcatgta
     2401 ggttttgtag aatcaataaa tatgatagct gatggattag gttggaaatt agataagata
     2461 gaacaaacaa aagagccaat aatgactaca gtagatagaa aatctaagta cggagaggca
     2521 ttggctggaa atgttgctgg ttgtagacaa tgtggatatg gatatgtaaa tggagaagtt
     2581 ttaattgaaa tggagcatcc acaacagata ataccagaag cagaaggtat taaaacagga
     2641 gactatgtat caataaaagg tataccaaac attgatttac aaataaatcc tgaaatacct
     2701 ggaggagtag gtacttatgc aatgatagta aactctattc cattaataat aaatgctaga
     2761 ccaggtctta aaactatgtt ggatatacca gtacctagag caataatggg agatataaga
     2821 aatcagatag aagttgaatt ggaagaagaa tcaaaggcta attagtaaga taacagataa
     2881 ctaattagta agatagcaac tagctaatta gtaagataac agttagctaa ttggtaaaat
     2941 aatgtttaat ttaaaataac agtaaattaa ttagtaaggc aatagtcaat taataagggg
     3001 atgaaaaaat ggatgctaaa attaatgatt gggttattat acacaatata gtattgactc
     3061 cagaagaaag agctccacaa gttccagagg atactaagaa agtatcctta gaaatgtggg
     3121 ttaaaggttt tatacaaagt gatgcttcta taggcgactt agtagaagtt aaaacaatta
     3181 caggtagact tgtaaaaggt aatttactta aaataaatcc atattatact catgattatg
     3241 gtaaatgtat accagagtta ctccaaatag gaatccaagc taaagaaata ttatttggag
     3301 gtgtatataa tgaataattc aacatcaatg aaagatatga gttatcaagc tgttatggga
     3361 agaaataacg aaataatgaa aaatgcaatc ggattggatt attcttcttt tgaacaagag
     3421 ggaatagggt ttgattatga gaaaatgatg agtgaaacag gatatacatt acaagatatt
     3481 gaagcaattc aatcacagta tgctgttgga aatactcctt taatagagct taaaaatttg
     3541 actaaattag ctagaaaatg tgctaaagaa ggtaaaggag ctagaatatt tgtaaaagat
     3601 gaagcaatga atgcatctgg tagttttaaa gcaagaagag cagctactgc agtatatcat
     3661 gccaaacaaa tgggatataa aggtgtaata gcagctacta gtggtaacta tggtgcagct
     3721 gtagcatcac aagcggcaat gcagggatta aaatgtataa tagttcaaga atgttatgat
     3781 tctaatggtg taggtcaacc tgaaattata gaaaaagcac gaaaatgtga ggcatatgga
     3841 gcagaagttg ttcagttaac tgttggacca gagttatttt atacgttctt agttttatta
     3901 gaagaaacag gctattttaa cgcatcatta tattcaccat ttggaattgc aggtgtagaa
     3961 actttaggtt atgagttagc tatccaattt agagaaaagt ataaaaaaga ccctgatata
     4021 gttgtttgta caaatgcagg tggtggtaat cttactggaa ctgcaagagg attgataaaa
     4081 gctggttcta ttaatactaa agtagtagga gcaagtgtag atttaaaagg attacacatg
     4141 gctagtgata atcaatttaa taaaaaatca tttacaacag gacatacagg ttttgggata
     4201 ccatactgta catggcctga taggtcagat gtacctaggt ctgcagctag accacttaga
     4261 tatatggata gatatgtatt agtaaaacaa ggagaagttt tttatacaac tgaattattg
     4321 gcacagctag aaggtataga aagaggtcca gcaggaaata catctttagc agcagcattc
     4381 tctttagctc aagagctaga tgaggataaa gctatagtgg tacaagaaac agaatatacg
     4441 ggtgcaggta aacatatatg tccacaactt acctttgcaa gagaaaatgg aatagatata
     4501 aaatttggaa atccaagaga agaaattgca ggagtaaact taatattacc agaaagacca
     4561 gaattattga aatgtgttga tattgatatg aataaaataa gaaagtcatt tataaaaaat
     4621 tgtatatcta ataatcacat tgataatatt gataagctaa gcaatcgaga tatagaattt
     4681 ttaatggaag aagtaaaaag ttcaagagat tttgttgttg atgtattaaa taatttatag
     4741 taattattgt tttttaattt aatataaaat aaaaattcag aaaattcgat taaatcgata
     4801 cggatggagg aatgttcatg aaaaaaagag aggatgattt tgaagttaga agaaaacatc
     4861 ttcaagaact gagtgatgat gagttaaaag aaaggttttg gagtttagct acccagatag
     4921 tagaaccaat gatagaactt ggtaaaaaga atacaacacc atcaatagaa aggtctgtct
     4981 tgttgagaat gggattttct tctcttgaag taaaacctat tttagaaggg gttatggaaa
     5041 gaggtcttat aggtaaagga gcagggcatg tagtttataa attggctaaa tctaaaaata
     5101 taacggttag agaagcaggt ttgttattag ttaagggtga atattgggat gaagttacat
     5161 gtttatttag agaaggggta gaatcatgtt aaaggaaaat aaaaaattgg atatagacta
     5221 tatattaaaa gatttggata aatataaacc aaaacgaaga ggttgggttt ggagagaaca
     5281 cttggaaaat cttgaaatgg gaccttttaa atataaagac tgtacaaaac ctttaaagaa
     5341 gagtgtaggg ctaccatcat ctaagtattt caataatata gaccctcaac caagtccagt
     5401 tattactact gaaatagctt ctggtagatt tgaggatgat ataagaagaa tgagaatggc
     5461 agctcatcat ggagctgacc atctaatggt tatcagaact gctggacaat cacattttga
     5521 tgggcttata gaaggaactc cacaaggtat aggtggagtt ccaataacaa gaaaacaagt
     5581 aagagctcaa aggaaggctc ttgattttat agaagaagaa gttggtagac ctattaatta
     5641 ccattcatat gtaagtggtg ttgctggtcc tgaagtagca gtaatgtttg ctgaggaagg
     5701 agtcaatgga gcacatcaag acccacaata taatgttctg tataggaata taaacatggt
     5761 aaggtctttt gtagatgctt gtgaggcaaa gaaaataatg gcttttgcca atatagctca
     5821 aattgatgga gcacataatg ctaatgctac tgctagagaa gcatggaagg taatgcctga
     5881 acttatggtt caacacgctt taaactctat attctcagaa aaaataggta tagataaaag
     5941 taatatatgc ttatcaacag ttccaccaac agcacctcca gcaccatgtt taaaaataga
     6001 cttaccttac gctgttgcac ttagagagtt atttagtgat tataagatga gagctcaaat
     6061 gaatactaaa tatatggaat catcaactag agaagcaaca gttactcatg tattaaactt
     6121 attaacatca gtattaacta gagctgacat acaatcaact ataacacctg atgaaggtag
     6181 aaatgtacct tggcatatat acaatataga agcatgtgat acagcaaagc aagctttagt
     6241 tggtatggat gggcttatgg atatgataga acttaaagat gtgggtgaat taagagacaa
     6301 agcaagagaa cttaaagaaa gagctgtatt atatatggaa gaaatatctg aagttggtgg
     6361 atattttgaa tctgtggaac aaggattttt tgttgattct ggaaattatc cagaaagaaa
     6421 tggtgatgga atatctagaa aaataaaagg tggagttggt gaaggtactg tttatgaaag
     6481 agaagaagat tatttagcac ctgtaactgc tcactttgga tacaataatg tagctcaata
     6541 tgatgaaaat gctatagata atccatcaat gttaatagat ggatgtacat ttgaaaatcc
     6601 agacaaaata atatatattg atgagcttga cgattttgat aatgtagaaa atagactaaa
     6661 agaatcatat gagtacagaa atggaactaa aattaagcct gaaatggaat ggtgtgcaga
     6721 tggaattgta atgataacaa tgatgcttcc tactgataaa agaacagcag agtttgcagc
     6781 tttggaattt gtcaaaaaaa tgaacttaca agaaattgag gtcataagta gagaagttat
     6841 gcatgaatct gaagggacta gaatagaagt gaaaggaaga gttccgtttg atatagattt
     6901 aaacaattta gtaataccag aagaacctaa ggtactaact gatgaagaaa taagagcaga
     6961 tatagaagaa aaacctatga aaatagtttc agctacggtt ggagaagatg aacattcagt
     7021 tggtttaaga gaaataatag atataaaaca tggaggtatt gaaaaatatg gcatagaatg
     7081 ccactatcta gggacttctg tacctgtaga aaaattggta gatgcagcga ttgaactgaa
     7141 tgcagatgca atacttgcat ctacaataat aagtcatgat gatattcact ataaaaacat
     7201 gaaaaaatta cacgattatt gtgtagaaaa aggtataaga gataaagtaa tgattgcgtg
     7261 tggaggaaca caagtaacac ctgaaatagc tgttgaacaa ggcatagatg ctggatttgg
     7321 aagaaacagc aaaggcatac atgtagccac tttcttagtt gaaaaaagaa gagaaatgag
     7381 taataaataa tggaaaatag caaagtttct ttaaaaatag atgtgttggt agctgaaatt
     7441 ggaagtacta ctacagtggt aaacgctttc cacaatatta atactaacaa tcctatattt
     7501 ttgggtcaag gtcaagctcc aacaacagtg tttgaaggtg gcgatgtaag aaatggctta
     7561 tcaggtgcta ttaaagattt agctaataaa ttatctgtag atgatataaa gtataatgat
     7621 atgtttgcaa cttctagtgc agctggtgga ttaaaaatga ctgttcatgg attagtctat
     7681 gatatgacag taaaagcagc taaagaagca gctttagggg ctggagctgt tattcgtcaa
     7741 ataacatcag gtagaattaa aagaactgat ttaaataaaa taaaagaaat aaacccaaac
     7801 ataattttaa tagctggtgg agtcgattat ggagaaagag atacagctat ttataatgca
     7861 gaaatgatag cttctatgaa tctaggcata ccagttatat atgctggtaa tgttgaaaat
     7921 caagaggaaa taaggttaat atttgaagat actaattata agctttatat aactgaaaat
     7981 gtatacccta aaatagattt attgaatata gaacctacaa gaagaatcat tcaaagtgta
     8041 tttgaagaac acataactac agcaccagga atgaaataca ttaaagaaat ggttaatgaa
     8101 aatattactc caaccccagg agctgtaatg gaagcttcta aacttttata taaaaatatt
     8161 ggagatttat taactctgga tgtaggtgga gcaactactg atgtacattc tgttacagat
     8221 ggaagtgact atattaataa aattttggtt aatcctgaac ctactgcaaa gagaagtgtt
     8281 gagggggatt taggtgtata tgtaaatatg aagaatatag tagaagttat aggtaaagaa
     8341 aacttacaaa gtgaactatc tatagatatt gatgcagtaa tagagaatta tcctccaata
     8401 ccaaaatcaa aagaagaaat attatttgtt gaaagattga caaaagaagc tgttgttaaa
     8461 gctatgctaa gacattcagg aaaaataaga aatatctatg gaacaactgg aaaagtcaaa
     8521 attgcagaag ggaaagattt aacagaagta agatatatag tttgtacagg gggagcactt
     8581 actaggctcc ctagtagaat tgaaatatta gataatatgc tcaaatacaa taaaaataat
     8641 gaattactat ttccaaagga aaaaacaaag atattaatag ataatgacta tattatggca
     8701 tctatgggtg ttttatctaa aaaatatgaa gaagcatctc ttaagctcct gttaaaaagt
     8761 ttgaattttg aggaggaaag attatgtatc ctaggttaga aatagatata gaaaagttaa
     8821 agcataatgc aaaattaatt tcgcagatgt gtcatgaaag aaatattaag atatcttttg
     8881 ttactaaatc attttgtgca caaaaagaaa tagtagaaga aatatgtagt gaaggaatag
     8941 accatatagc agattcaaga atacaaaact taaaaaattt acaagatata aatctaccta
     9001 agatattgat tagaatccca atgttaagtg aattagaaga agttatcaat tattgcgata
     9061 ttagttttaa ctcagaatta ggaacaataa agaaattaaa tgaattatgt gaaagtaaaa
     9121 atattattca taagatagtt cttatgtttg acttaggtga tttacgagaa ggatactttt
     9181 atgaagaaga cttttttaat aatgtaagag agatagtaag attaaaaaat attgaaataa
     9241 ttggtatagc tacaaactta acctgttatg gagctattat accatctagg gaaaatatag
     9301 gcagactggt aagtatagca aaaagaatgg aagaaaaatt tggaataaat ctagaaatag
     9361 tatctggagg taattcaagt tctattcatc tgttgataaa taataatatg ccagaaggta
     9421 ttactaattt gagaataggc gagtccattc tacttggtag agaaacggca tacggtgaaa
     9481 atataaatgg aacatatcaa gatgcattta aattaatatg ccaagtagtt gaatgtaaag
     9541 aaaaaccatc agttcctatt ggagaaatag gagttgatgc ctttagaaat aaacctgttt
     9601 atgaagataa gggaatctta aaaagggcaa tcatagctat aggtaaacaa gatataaata
     9661 ttgactcttt aattccaata gacactgata taaaaatatt aggggcaagt tcagaccaca
     9721 tgatactaga tgtaagtaac acaaaatatg attataaatt aggtgacaat ttagaatttt
     9781 tattaactta tgggggtatc atgtcatcta gtacgagcaa atatgttgca aaaaaaatta
     9841 tagtgtagtg tttaaataat taggaggaaa ctattttatg aaagagcgag aatgtagaaa
     9901 agcaagtctg tttgatgcaa tgataccaat tacttgtttg atattatttt taagtctagg
     9961 agtattagtg tatggttcat cacctcatgt accactaata ggagcagcag cagtagcagg
    10021 acttgtggct gtatatagac ttggatttaa atggaaagag ttagaattaa gtatgtttaa
    10081 tagtataaag atggcaatgc aagcaatctt aatcataatt ataataggcg tacttatagg
    10141 aacttggata gtaagtggag tagttccatg tatgatttac tggggactaa aaattttatc
    10201 accaaatatt tttttagtag catcaacatt agtctgtgca atagtatctc ttgcgacagg
    10261 ttcatcttgg agcacaatgg gtacagtagg agtagctctt ttaggaattg gtcaaagttt
    10321 aggtatgcca attggattaa tcgtaggttc tataatatct ggagcttact ttggagataa
    10381 actatcacct ttatctgaaa cgactaactt agctccagca atggcaggaa cagatttgtt
    10441 tacacatata aaatatatgc tatattcgac tataccatca ttattaattt gtttagtaat
    10501 ttatggtgta attggaatga agtattcagg tcaagcacta gatataaaac aaatagaatt
    10561 aattaggtct acattagatt ctacatttaa tactttatca ccagtattat tattagctcc
    10621 agcaatagta attggattag ttgtatttaa agttcccgct ataccagggc ttatagtagg
    10681 tgctgtattg ggagtattgt ttgcagtagt ttttcaagga gaatctatga atactatatt
    10741 agatgcagca caaaatggat atgtttcgtc aacacaaata caggaggtag atgctttatt
    10801 atctaaaggt gggattatga atatgatgtc cactgtatcc ttaacaatat gcgctttatc
    10861 tcttggaggt attttagaaa aaacaggtat gcttgaagta gtagcatctt ctttactaag
    10921 attagcaaag ggtgtatttg gaacagtatt atgtactatg gtaacttgta caattaccaa
    10981 tataatagca ggtgaacaat atctatctat agttatacca ggaagaatgt ataataagga
    11041 atataaaaag agaggtatac atcctaagat gttgtcaaga gctttagaag atagtggaac
    11101 tttaacttct ccattagtac catggaatac atgcggggcg tatataacag caacattagg
    11161 tgtatctgca ctaacatatg gtccatacgc tttattaaat attataaatc caatagtatc
    11221 tttagtatta attgcatgta agtttaaaat atcaagaata gaagatgaac ctgaaacttg
    11281 tttaaatttt gatgtttgat attttaattg tttgtataaa ttttttatag aaaaagattt
    11341 cttttagact tgcctaaaaa ttaggcaagt cttttaagta tgttctgatt ataagagcac
    11401 tgtgtgacaa gaatgatgag attatttttg ctatactttc gatataagaa atggaaggag
    11461 gttatgtttt atattcatat gaaataatta gttacacagt gaattttcgt ataatttggt
    11521 ataaaaagat tagaaatgaa aataaagtaa tggaggaata aaatgactag aatatccaat
    11581 ataacaatag ctgaacaatc tgagtactgt tttttggcaa tacgaaaaac aattgacttt
    11641 atggtagaat tttcagagtt ttcaaaacaa agttttgaaa aaatttctaa atatctggag
    11701 gagagaggaa ttttatcttc aggagcaccg attgtatgtt ttcataacat ggatttagta
    11761 aaattggatg ttgaagtagg gtttcctgta gccacttata tagatgggaa aaatgagatt
    11821 ctacaaaaga ttgttcctgc acagaaaata ataactgcaa ttgatttagg accttatgaa
    11881 ttacaagacc ctactttaga agacttattt tcatgggcaa atagcaatgg atataaactt
    11941 catggcgata tatattacca gtatttaaat gatatcaatc gtccagctag tgaatatctt
    12001 acgaaaatga tgttacccat tatatagaaa gttaaagatt atcgtttgtt agttgataat
    12061 tagaagaaca tttactgcta ctacataaat tttatcctaa ataatatcaa ggtgatttaa
    12121 tttatgtatt ctaatgtaac cccaatatat tgttaaggtc tacttaagat gctcaaaagg
    12181 ctatgcttaa tttattgtta tataatttgt agagtggtat aatttagagg taatattata
    12241 tagatatata gggggaaaat atgaaaatat atgatatatt aacacgtttt tatattcaag
    12301 atatagaaaa agccatacct ttttatgaga atcttttaaa agaaaaatgt tctttgcgtt
    12361 tttcctataa agaagttggg ttggaattag cgcaaatagg aaatgtttta ctattatctg
    12421 gttctgatga tgctcttaaa ccatttatag aaacaaaatc tacttttatg gttgattctg
    12481 ttgatgaatg gagaagttat ctattggata atggagcagt tgtagtaaga gataagaaaa
    12541 aagttcctac aggatataat atgacattga gacatccaga tggtacaatt atagaatatg
    12601 ttcaacatac aaagcaggat tagttaaaaa tatattttac tactaagtaa ctaattataa
    12661 tatattcttc ggaaaactgg aggtaatttt atgaacgaaa aatggttgga atgggcgata
    12721 gaattgcaaa gcattgctca agccggaata acttatggga aagatatata cgacatagag
    12781 cgatatgaga gaatacgaga aatttccgcc gaaatgatag catataaaac tgacatatca
    12841 acagaaaaag taagaaatct tttttgcaat gaaagcggat accaaactcc aaaacttgat
    12901 accagagccg ttatttttga aaatggaaaa atacttcttg ttaaagaaaa tacagggaag
    12961 tggtcgcttc cgggtggttg ggttgaagta aatttatcag tcaaggaaaa tactattaaa
    13021 gaggtcaaag aagaagctgg attagatata accgccgaca ggattattgc aattcaagac
    13081 cgtgcgaaac acaatttacc tatatatgct tacggagtat gcaaaatatt tgttctttgt
    13141 accgttatcg ggggtacatt caaagaaaat atagaaacta cggagttttc ttatttctcc
    13201 gaaaatgaac ttcctgaact cgccacagaa aaaaatacag aagaacagat taaaatgtgc
    13261 tttgaagcat gtcgtacaga cacttggaca gtgattttcg attaggttaa ttaaggagaa
    13321 attaatggat tacaaagaaa tcgtctttga tgtagacggc acattgattg atactgaata
    13381 cgccatttta cactctctac aagacacgat aaaacaaatc aatagaaaga taattcctat
    13441 caaagaattg tcttttgctt taggaataac aggcgaggac gctttacgtt atttgaaaat
    13501 cgaagatata acaattgctc tatcaatgtg ggataggaat atggaaaagt ataagaatac
    13561 agttcttgtg ttcaaagaaa taaaggaaat gcttactgaa ctcataaaat taaatattgg
    13621 cattggaatt gttacatcca aaactcatga agaatttaga aacgattttc tgagctttga
    13681 catagcacca tactttaata ctgtagtatg ctccaatgac actctagagc ataagccaaa
    13741 agcagtacca ttattgaaat acatggaact gacaaaatgt caaaacaatg aactgcttta
    13801 tattggggat agtatatatg caatgtgcct atcgtgcaaa tgttgatttt ggactggtaa
    13861 aatggggttc tggcgataga gtaagcgctg taatatcttt taccacacca ttggatttga
    13921 taaggcaact atctgtatca agcaactaac aatcatagaa aatagcaaag tcagccaagc
    13981 cagtcaatgg tcaagatgaa cggcgcattt catgtgccac cgttgacagc accgtctgat
    14041 tttgctgacg ggcaatcaag atgggaaagc cctaaaatgg cttcccgcct atttcaattt
    14101 tgagaggaaa atccgtctgc tttttcgtga gtgtggctac aagttggagc gtaggatgag
    14161 ggacgggagc tttcatatac acatgtatgc tgctgaaacc cgccctgcgc ttgtggcttc
    14221 acgctatgca atcacagccc tgttttcctg ccgcttcaaa tgcccttgcc ctccctgacg
    14281 gcaacggcat ttttacggca acgccaacag agcgtataac acactacact ttgcaagtaa
    14341 agtcgtgtgc caaggggaaa gcccctttgg aaattccaga caacaaaaca gtctgaacac
    14401 ctcgcgcttt ccctgtgctt gatgttcagc ttttatttgt tgtaagcagc cccgtttcgt
    14461 ggtctgcgtg tagttccttg taaactgacc taagattcat taaatataac taagttataa
    14521 ttaatataat gttgcatgga taaaaggagg gaagctgcaa tgtcttacaa agtaattaat
    14581 gtcataactg ctattgacta tatagaggag catttatcag aaaagctgga tttagatata
    14641 gttgctaatg ctgttcatta ctcaaaatat cacctgcata gaactttcac aacttctgtt
    14701 ggtctaacaa tgcatgatta cataaagcgg agaaagttga cagaagcagc aaaattattg
    14761 gtattttcaa agaaaccaat tattgaaatt gcattgatag ctggatatga aagtcaacag
    14821 gcattcactt ctatatttaa agctatgtat aaaaaatctc caaataaata taggaaagaa
    14881 caagaatttt atcctttgca gctaagattt gttttgaaaa aagataacct attttcaaag
    14941 aatgtactag agttggaaaa agaaattaaa cttgcaagta tgtctgacat accattatgg
    15001 atgaatttag tgagattagt catagatgga tttcccaatt tgcaagagga agaatatata
    15061 tatcaattag aacagtatat tttagaagaa agagctttaa tattaaaact taataatgtc
    15121 gctattgcaa atatggtgtt taatagagaa actagaagta ttgatttttt tggaatacat
    15181 ccacagtatc gtaattcaga cattgcacaa gtgtttttga agaaagttat tgaagatttc
    15241 ttgatagata cagatatttc aattactacc tttcgagagg gtgacaaagc tgatacagga
    15301 catagagata tgataaagaa gttggggttt gcagaagcag agttgcttgt ggaatttgga
    15361 tatcctactc aaaaatttat tcttccctta agttaaatag caagaatgat taaaaactcc
    15421 aaaacacaat taagtataat gattcagtgt aagtacttaa ttaacaaagg aggatttaaa
    15481 tgtctgaaaa taaacaagta gtaaattgcg atattattgc atctaattct aaacataata
    15541 tgtgtgaata tgatgcagag ttattaactt tttttccaga tgagattgca catctaatag
    15601 agataaataa caagctagac aatgctttta agaaagctga aaatcttgtt gacaagcttg
    15661 ataaggatta tatggacgct aaaatgtata tggtaaaaaa tcgtggtgaa atagacccac
    15721 atgaaatgtt tcaaaatgaa caaggactaa aacaaataga taactatggg gcttttatgg
    15781 taaaagttcg agataagatt aataaaatca aagattctcc atactttgct agaatagatt
    15841 ttaggctgaa agatatggat gatgaatcta agtattatat tggtagattt gcatttgatt
    15901 atgaagatga gcttataata cttgactggc gttctccaat tgccagtatg ttttatgatt
    15961 atgaaatcgg aaaagctgga tatgatgctc caataggatg ggtggatgga gaaataacaa
    16021 gaaaacgaca atttaaaatt aaaaatggaa aattagaata tgcactagaa agttccatta
    16081 atatacaaga tgatattctt caaaaagaac ttagtcacac ttcagatgaa aaaatgaagt
    16141 caatcatttc aacaatacaa aaggaacaaa atcaaattat tagaaatgat aaagctgata
    16201 cactgattat tcaaggggta gctggttctg ggaaaacatc tatagcatta cacagaattg
    16261 cttttcttct ttatcgattt aaagataaaa tatcagcaaa taatgtaatt atcctatcac
    16321 ctaataaagt ctttggagac tatatttcca atgtccttcc tgagcttgga gaagagccat
    16381 tatgtgaatt aagttttgaa aatatagcag aggtacaatt agatagagtt attaattttg
    16441 aaagtgagaa agacccatta gaaataaatg atgctaaatg gtctgaaaga gtgcgtttta
    16501 aatctacact tgattttgta aaacttattg atgattatat aaaacaaatg ccaaataaga
    16561 tttttattcc aaaagactac acttttggaa gttttacagc taaaagtgac tggatacaga
    16621 gtagatttga ggcatataac agatatccag ttaagaagag attagagaag gtagctgaag
    16681 acattcatta taaatttgaa tcagataata ttatggaaga agatttacca aaggtaaaaa
    16741 gtatacttaa aagtttgaat ggaatgttga caataaaaaa tactcttaca ctatataagg
    16801 atttctttaa acaaatgaat atatcaaaca tgtttgtgat ggcaacaaaa aaaactcttg
    16861 aatggtcaga tgtataccca tttatatata ttcatgctgc atatgaaggt atacaagaag
    16921 ataagattat aagacatgtt gttattgatg aaatgcagga ttacacacct attcaatatg
    16981 ctgtaataaa cttacttttt aagtgtaaaa agactattct tggagatttt ggacaattgg
    17041 ttaatcctaa tcacactcat acactagatg atatgaaaca gctatataat gatggggaac
    17101 tagttatgtt gaataagagt taccgttcta cttttgagat tattaatttc gccaaaaaga
    17161 ttcaagacat atcttcatta gagcctattg aacgacatgg tgaagaacca gctttattaa
    17221 aatgtaataa cgagcaagat gaaataaata agataaaaat agaaattgaa gaatttaaaa
    17281 agagtgataa tgcgactctt ggtattatat taaagacaga taatgacgct aaagctgtat
    17341 atgatgcgtt aagagaaaaa tatagtgttc acttaatatc ttctgaaagt tctagcttta
    17401 caaaaggagt atccataact tctattaaaa tgtcaaaagg tttagagttt gatgaagtta
    17461 ttgtaccttc agtaaataat aaaacatatt atagtgatta tgaccgcagt ttgttatata
    17521 ttgcttgtac acgagctatg cataaactta agttgacata tactggtgaa ttgactcaac
    17581 ttatagatat gtagattata tacttattat gtaagtgttt acttatataa atataacctg
    17641 taaataaaat tcattaataa atcaataaaa ataatatttg taaaagtaaa agctataaat
    17701 tatatattaa attttataat ttatagcttt tataatactg cttatcttaa tcctaccaat
    17761 agcaaaataa ttttaaacat gccatatttt ataaatgtaa taacaattat acaattaaaa
    17821 taactttccc aaactaactt gacattta
//
LOCUS       DAEORZ010000084        17542 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEORZ010000084 DAEORZ010000000
VERSION     DAEORZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.084"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            23..1222
                     /locus_tag="KRH39_002433"
     CDS             23..1222
                     /locus_tag="KRH39_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBG8512331.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVNDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDDEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYIDTLEKHKALIIDIRGNLGGSDSYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKQYTNARNIKLNPVENLPTSVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            1389..2174
                     /locus_tag="KRH39_002434"
     CDS             1389..2174
                     /locus_tag="KRH39_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8512332.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            2343..3908
                     /locus_tag="KRH39_002435"
     CDS             2343..3908
                     /locus_tag="KRH39_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG8512333.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            3922..5253
                     /locus_tag="KRH39_002436"
     CDS             3922..5253
                     /locus_tag="KRH39_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG8512334.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILEEMIVANKDFWPTFK"
     gene            5267..5755
                     /locus_tag="KRH39_002437"
     CDS             5267..5755
                     /locus_tag="KRH39_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512335.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSSF
                     TNISFSQLLNNDVNASLNLISNFNAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            5769..6236
                     /locus_tag="KRH39_002438"
     CDS             5769..6236
                     /locus_tag="KRH39_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8512336.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            6239..7393
                     /locus_tag="KRH39_002439"
     CDS             6239..7393
                     /locus_tag="KRH39_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8512337.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            7709..8461
                     /locus_tag="KRH39_002440"
     CDS             7709..8461
                     /locus_tag="KRH39_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512338.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            8462..10369
                     /locus_tag="KRH39_002441"
     CDS             8462..10369
                     /locus_tag="KRH39_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG8512339.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEVYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNVYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            10521..11201
                     /locus_tag="KRH39_002442"
     CDS             10521..11201
                     /locus_tag="KRH39_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8512340.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            11195..12214
                     /locus_tag="KRH39_002443"
     CDS             11195..12214
                     /locus_tag="KRH39_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8512341.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LSLDS"
     gene            12645..13805
                     /locus_tag="KRH39_002444"
     CDS             12645..13805
                     /locus_tag="KRH39_002444"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBG8512342.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISENGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            13807..14205
                     /locus_tag="KRH39_002445"
     CDS             13807..14205
                     /locus_tag="KRH39_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512343.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            14467..14775
                     /locus_tag="KRH39_002446"
     CDS             14467..14775
                     /locus_tag="KRH39_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8512344.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            14864..16210
                     /locus_tag="KRH39_002447"
     CDS             14864..16210
                     /locus_tag="KRH39_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8512345.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            16424..17521
                     /locus_tag="KRH39_002448"
     CDS             16424..17521
                     /locus_tag="KRH39_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG8512346.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLKH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
BASE COUNT         6814 a         1761 c         2999 g         5968 t
ORIGIN      
        1 tctgaaagtg gatgagaatt acatgaaaaa gaaagtatta tttttagtaa ttattttagc
       61 atttataatc ttattaggta attctactta ttctcaaaag aatcagttgt ctgaagagga
      121 aaaggtgaat gactttaatt atgtgtataa tgttgtaaag acaggatatc catatctagg
      181 tgtaaataag aggcttaata atattgattg gttagcaaat aaggatgaat atacaaaacg
      241 aataaaaaac actgaaaatg atgacgaatt tatagaagaa ctgtcatcta tattatctga
      301 tttaaataat aaacacacag aagtaataga taataagaaa agatatgaac tttttaagaa
      361 atcatactct aataatgatt ggtatgattt tcttaatgat aaaaaagttg tagatagata
      421 tgactctatg aatcttaaaa taaaaatgcc agatgatatt tttgtaaaaa aagaattaat
      481 tttaaaggat gttattaagg gtcagattgg atatatgtat ttaccatcaa tgtcatcaaa
      541 gaatggctct ataaataatg atttaaaaat aattggagat tatattgaca ctttagaaaa
      601 acataaagct ttaattattg atataagagg taatttaggt ggaagtgata gttattggca
      661 gggaatcgta tctaaactca taaaaaatga tgtcaaaata aatggttata gaatttatag
      721 aaataataat gaaatagtaa aacaatatac aaatgcaaga aatataaaat taaatcctgt
      781 agaaaattta ccaacaagtg taaaggaaaa tgcacctaaa gaaattataa aagattttaa
      841 tgattttgaa gatacttcct atactataaa atctacagat gattcaaaat ttaagggtaa
      901 tatatactta cttgttgata gaaaaatata ttcatcatca gagtcatttg caatgttttg
      961 taaagaaact aaatttgcga ctttagttgg tgaaactact ggcggagatg gagggtgtat
     1021 agaccctgta ttgtttaatt taaaaaatag tggtctaata gttagaatgg caagttgtat
     1081 gtatttaaat aagaatggaa tatgtgatga ggaatttaaa actattccag aatttaaagt
     1141 aaaagattgt aaaaggactg ctgattttaa agatgataat tgtataagaa aagcactaga
     1201 acttgaaaat attaattatt agataaaaga ataaaattaa aactacattt ttttaaacat
     1261 caattatagc agtatttttt atagtcaatt ttatgggcta aatagtaaat ataagaatac
     1321 gctgtaattt aatgttgaat attggtataa tatttatgac gatttttaat taaagggggt
     1381 aatagtttat gagattagaa gaaatgattt ttttaaatta taataaattg aatgatacag
     1441 atttattaat atggaaatat ataactgcaa ataaaaaaca ttgtagtaag atgtcaataa
     1501 atgaacttgc tatgaaatgt aatgtgtctc gtgcaactat tacaagattt gttaaaaaat
     1561 tagatttaaa aggtttcagt gagtttaaag tattattaag ctgggaatca gaaaaggttc
     1621 atgtcataga aaatcagtca tttgatattg catgtaattc aataattaag tatgttgaag
     1681 atcaaaaaaa taaagattat gatgcattgt gtaaacttct ttatgaatca agaacaatat
     1741 acgcttatgg ttcaggtgat attcaaaatg tagttgcaaa acagataaag agaatgttct
     1801 tatcatgtca agaaataata tatgattttg agggaagaac atttgatgat tctttttaca
     1861 atattgtcag taaaaatgat gttatgatta ttatatccct aagtggtgag aatgagaata
     1921 cacttaatat tgctagaaga ttaaaattaa taggaactaa aataatttca attacagagt
     1981 ttaaaaataa tacattgact gatttaagtg atgaatcaat ttatatttca gcgactaata
     2041 tctcattttt acaaatgcac cctagttata aattgacaat gttatatttt atattgattg
     2101 agttattatt tattaaatat agtatttata aaaggcatag aatgattaca gaagggattg
     2161 attgcaaatt gtagtcattc ttttttttat gagaatttga acataaaaat gtgcaaaaac
     2221 ttatttgact gtgaataagc tccgaatgta ccctaaagat tgtatatatt tattaacctg
     2281 ttaaaataat agatatctaa ggccaaagat aaactaaaaa tatgaggagg ggagtaattt
     2341 atatgatgaa gaaaatacag cgttttggag gggcaatgtt tacaccaaca ctattatttg
     2401 cttttgctgg gataatggtt ggattctcaa ttgtatttca aaatcagtca attatgggtt
     2461 cacttgctac accagaaaat atttggtacc aattttgggg tgtaatatct agtggagctt
     2521 ggatggtatt taatcaattg ccattattat ttgctatttc tttaccaata gcacttgcaa
     2581 aaaagcaaca agcaagagct tgtatggagg ctttagcaac gtatttgact tttaattatt
     2641 ttgtaggttc tatgttaagt ttttggggaa aaagctttgg agttgatttc gcagctgaaa
     2701 taagtgctgg aagtggactt gtaagcattg ctggtattaa aacattagat actggtatgg
     2761 ttggtgcttt gttaatatca ggtattgtca tttatattca taatagattt tatgataaag
     2821 aactgccaga ttttattgga ttatttagag gctcatcact tgtagttgca atttgtttct
     2881 ttgttatgat accagttgca ttattaactt gttttatttg gcctcatatt caaaatgtta
     2941 ttagacattt gcaaacgttt tttataaata gtggaaatat aggagtttgg tgttatgcat
     3001 tcttacaaaa gattttaata ccaactggat tacatcattt tgtatatgca ccaatttgtt
     3061 atgatagtgt tgttgttcca ggtggtacat cagtatattg ggctactcat attcaggatt
     3121 ttcaaacttc agctaagact ctaaaggaaa tgtatccaat cggattttca ttatccggat
     3181 tatcgaaggt gtttggttca ttaggtgtat ttggtgcttt ctatgtaact gcaaagccag
     3241 aaaaaaagaa aaaagtatta ggtttaatga ttcctgctac tcttactgca gtattaactg
     3301 gaattactga gccgttagaa tttacatttt tatttgtagc accactttta ttcctagtac
     3361 atgcattttt agatgcatgc ttgcaaacga tttcgtttgc cctaggagtt gttggtgatt
     3421 ttggtggagg aattattaac tgggttgtat taaattggct tcctcttgga atgtatcact
     3481 ggaaggtata tgttgttcaa gttgttgtag ggattatatt ttcatttatc tggttctttg
     3541 tatttacttt cttaattaag aagtttgata tgaaaactcc tggtagagag gaagatagtg
     3601 aagaaacaaa actatatact aaaaatgaat acttagaaac taaagatgaa aaaggaaata
     3661 aattgagtaa agcctctcaa caagcatctg aatacataaa attagttggt ggagcagaaa
     3721 atgttgtaga tgttacaaac tgtgctacga ggttgagatt aaccttgaaa gatgactcaa
     3781 ttatttctaa agaagaagat tttaaggcag taggtgcaca tggtttagtg cataatggaa
     3841 aagctattca gattattatt gggctttcag taccaagtgt aagagaagaa tttgaaaatt
     3901 tattataaag gagaaggaaa gatggaaaga aaaagacaga ggatactaat tgctggtggg
     3961 ggtagtactt atactgctgg aattgtaaca atgttaattg aaagtgttgc taaatttcct
     4021 attgaatcaa ttaaattata tgataataat gatgagagac aaagaaaagt tgctgaagca
     4081 tgtgcaataa ttgttagaga gaaaaatcca gaaattaaat ttagttatac aacaaatcca
     4141 gaagaagcat ttactgatat tgattttgta atggcacaaa ttcgtgttgg tttatatgct
     4201 ttacgtgaac aagatgaaaa aattccacta aaatatgggg ttgttggtca agaaacttgt
     4261 ggggcaggtg gaattgccta tggattaaga acaataggtc caataatcga aatgattgat
     4321 tatatggaaa aatattctcc aaatgcttgg atgcttaatt attcaaatcc agcagctata
     4381 gttgcagaag catgtcgtgt attaagacca aattcaagaa ttattaatat ttgtgatatg
     4441 ccagtatgtt tagaggaaat atttgctaga gttttaggtt taaattctcg taaagacttt
     4501 gatgttagat attatggatt gaatcatttc gggtggtgga caagtattaa ggacaaagaa
     4561 ggaaatgatt taatgcctaa attacaagaa tattgtgcta aaaaaggata tgaggaattt
     4621 acacctcaag gtcaacacaa agaatctagt tggttagaaa ctatgagagc agcaaaagac
     4681 ttattagaaa tagaaccaac tacattacct aatacttatt taaaatatta tttaatggct
     4741 gatgaaactg ttgaacatgc taatccaaat tatacaagag caaatgaaat tatggataga
     4801 agagaaaagg atacattcga agaatgtgaa agaattataa aaaatggcac agcaagagat
     4861 acatggtttg atgcaagtga acattctcaa tttattgttg aattagcttg tgcattagca
     4921 tttaatactc aggaaagatt tttactgatt gttcctaata atggagctat tgaaaatttt
     4981 gcagatgatg caatggttga aattccttgc cttgttggaa aagatatggt tgaacctatg
     5041 tctataggta aaattccaac tttccaaaaa ggtttaatgg aacaacaagt tgcttctgaa
     5101 aaattagctg tagaagcatg gatagaaaaa agttatcaaa aattatggca gtcgtttact
     5161 atgagtaaaa ctgttccaag tgcaaaagta gcaaaagaaa tcttagaaga aatgattgtg
     5221 gctaataagg atttctggcc aacttttaaa taaggagaac aaaaacatga taacaaataa
     5281 aataaaaaat atcacagcag tattaaaaga tgaaaatata tttctttcag tggttatggt
     5341 ttgttttcta atattacctg ttttatatat agtgtattta gaattgacat catcatttac
     5401 aaatatttca ttttcacaac ttttaaacaa tgatgttaat gcaagtttaa atttaatctc
     5461 taactttaat gcagtataca gtgcatatgt tatttacaat ataaaaaaga ataaagagat
     5521 aaaagaaaac atgattgctt tctatttagt cttattaggg cagatattcc aaatttacac
     5581 aataactatt gttatcatgt ttacctatat ttataaattt gttggaatta aacaaatcat
     5641 aagcagtatt aaagaactac aatggggaaa gagctataag gctctttttt cctcaattgc
     5701 agtatgctta atatcgttat ttgtaatgat aattagaata aagttaatta tgtagaggag
     5761 tgtctataat gttctttaaa agaaaaaaag aagcaattac aattaattca tacattgatg
     5821 gaaaaataat taatcttgga gaaattcctg ataaagtatt ttcaactaag gtaatgggag
     5881 atggatttgc tatgatagca aaggacaata ttcttaaatc accgactaaa ggagtaataa
     5941 gttttattgc tcaaactaac catgctatag gaattaaatt gcaaaatgat gttcaaattc
     6001 ttattcatat tggattggat agtgcaacaa caaaaaaaga aaactttaaa ttagaagtta
     6061 aacaaggtca agaagttaat atgggggaaa agttagtaga aattgataat aagttacttg
     6121 aaaatacttt atatattcct ttagttattg tagagaatcc taatacggta aaatatcatc
     6181 tttcaaactt aaatcaggaa ggaaatagtg gtattaaagt tatcaatttt gattgattat
     6241 gattaataaa gcgattaaat ttaaatttat aatattctat tttttctcat atattatttt
     6301 tgcctttgct tcaacaaaat tcacaccttt cttatcaaaa ttaggttact cagcgtttga
     6361 aagaggaatt atattatcga gctatgcaat aaccaatata ttgtttcaat tattatttgg
     6421 agtattagct gataagtatc aaacaatgaa aaaaattgtt ttaattagtt tatcagttta
     6481 tggaattgca tcagttatta tgttttctac tgaatctgca acttttataa tatacttatt
     6541 acttgttgct ttatcaggag gattattaaa tacttgctgt agtttatatg atacatggat
     6601 aataggttgt ggagataata ttaataatta tttgtcattt attaagactt ttgggtcgat
     6661 tggatgggct attggaagta tggtagcatc ttacttgatt cttagattct catattttgg
     6721 attatctgta gcgattggtt taattgtagt attacttatt ataaatatac tattattacc
     6781 tgatattgaa aagattaata agaaaataaa tgtaactaat aaagatatta ttgaattatt
     6841 aaaagataaa aaatatatct tactagtatg tattttattc ttattatact caatggtggt
     6901 agcaaataac tgtactgtaa tagataaaat gatagcttta aatgctacaa attctcagat
     6961 atctttaaag tggtctttac agtcattgtt agagatacca acatatctgg ctggttctta
     7021 tttattaaaa aaatatagca gtttaaactt attaaaatta agtgctatta tgataacagt
     7081 tcagtttcta ttatttgctt taacaaatga ttcaaatatc attattatac tgtgtgtttt
     7141 tcaagtgttt tctactcctt taattttaat tacttcaaaa agattaatat ttgaagtttc
     7201 tcctaaaaaa cttagaagta gtagccaatt aatagcttta agtatattta tgggaggttc
     7261 atctctgatt attccaactg ttgctggttt tttgagcatt aatattgggt ataattatac
     7321 attaatggta ttaagtcttt ttggatgctt agcttattgc ttgatagtct tattgaatta
     7381 tcttcaggaa taattgtttt aatatatagt agtaatatca cttgtttata gtatctgtat
     7441 ttgccacaat caatgtgtat tttggttttt tatagagaag aataattgta tagattagag
     7501 gcttcttagg agcttctttt ctttgtttat acaaagaagt aaatcatgct tttattttaa
     7561 gatatttcca ttataattat aatagtcttc catatgttac aaaagtgtaa tgctaacagt
     7621 taaaatgtaa tatttactta aggatttatt tatataaaac ctttaaaata aatatatatt
     7681 aaaaaattag caactggagg ctattattat gaaagaaata ttaaaaataa aaaatatatc
     7741 aaaagattat ggaataaaag gatttaaaac aaatgtatta aaaaatatat ctcttacagt
     7801 caatgaaggt gattttatat caataatggg tccatctggt gcaggaaaga caacattgct
     7861 aaatttaatg tctacacttg acaaacaaac ttcaggagaa ataatactag atggaattaa
     7921 tatatcaaaa gtaaaaaata atgaactatc aaaattaaga agagaaaaaa tagggtttat
     7981 atttcaagac tataatctat tagacaatat gaaactaatg gacaatatag cacttcctct
     8041 agcattagga aaaaagaaaa gtaaggagat agaagctaag gtattttcta tagctaaaaa
     8101 atttggatta gaaaatcatt tagataaata tccatatcag ttatcaggag gacaaaaaca
     8161 aagaggcgca gcagctaggt cattaattac aaatccaaca gtcatatttg ctgatgagcc
     8221 aactggtgct ttagactcta aatcagcata tgaactttta gaatcattag aaaaaataaa
     8281 cagagaaaat aacgctacta ttataatgat tacacatgac cctctaacag caagttattc
     8341 aaatgaagta tatatgataa atgatggaaa tataaagtgt aaattaaaca aaggaaatag
     8401 tagaaaagaa ttttatggaa aaattatgga tatgctagcc tctatgggag gtgagatgta
     8461 agtgagtata cttggaattg cttataataa ttttaagagt aacataagaa cttatctagc
     8521 attttttata tctatggttt tttcagtagt tgtgctaact aattttgaat tattaagata
     8581 tggtgatgct ataaatgtgt tacaagatga aaataaaaaa tttacactat cagtgttagt
     8641 agctgtaata atgatactat cagtattttt atttttcttt atatggtatg caacaaatat
     8701 attttttaag aataggtcta aagaaattgg aatattgtct tttatgggat tagatttata
     8761 tactatagga aaaatatatt ttgtagaaaa tatgcttata ggaataagtt cttgtataac
     8821 aggaataatt atagggataa taacatctag attttttcaa gtagtgataa taaagttatc
     8881 tggatttaat ataaaggtat ctaatggact tagtatagaa gctatactat atgctacttt
     8941 aatctttatt tctatattta taataatggc tgtaaaaggt tttataacaa tatgtagaag
     9001 tagtgtaata aatctaatca atatatctaa aaaacaagaa aaaataccaa aggtaggaat
     9061 aggtatatat atattagcta tagtatcaat tatagtaata ggatatggct actatctttc
     9121 taccaatata agaactggag atataactag tgtactacct gttataggta taataatagt
     9181 aggtacttat ggattattca aatcagttat gcctgttata tttgatatta taatcaaaaa
     9241 taaaaaaata ttatttaatg gaaataatat aattgcaata aacaatataa actaccgatt
     9301 aaataaaaac tataaaactt atgctataat agctataatt ataactacaa caatatcaac
     9361 tcttggtgct tcagttgcga tgaagcgtat acataagaat gcccaagaac aaagaaatgt
     9421 gtacactatt agcatagttt caatggataa agatgaagtg aataaaaaac aaatagagaa
     9481 tataataaaa gatactaata agataaagta ctcagtaaat ccagaactaa caattttaaa
     9541 gaaaacagat ataaaagaag tttatgatac aacagatact ataatgaaat atagtgattt
     9601 ttgtaatact cttaaagtga atggcaatga taaagaatta aaagagtatg gtgaaagatt
     9661 agttagtgga aataatgtaa tagagttaaa aagccctcaa acgatagcat caatagctac
     9721 aaaagaagcc aagataaaac ttgatggtca gttatataat atttcaaggg gagaagtaaa
     9781 agttccagta ctagggacag gacttaatca aagtattata gtggtaaatg atgatacata
     9841 ttttaaacta agagataagg gtcaacaagt gtatttttat ggtgcaaagg tatttcaaga
     9901 agaaaatagt caagtaatgt tcaacaatat tgaaaagaat ttaagtttaa aaaatgtgta
     9961 tattcaaaat ggatataaag ctcaaggtga gagtcaatgg atgaagtttg catatgtagt
    10021 gcttgtgttt ttattcttag tctttgcaat tgtagctgga agtataatat atatgaaaat
    10081 atatagtgat gcatatgaag ataaagagaa atataagatt ttgttaaaaa taggtactac
    10141 agaaaaagat ataaatagag ctatacttaa agaagtagct attttttata ctttgccaat
    10201 gttaagtgca actatatcta gttattttgc attgagatta gcaggagatt tattgatgac
    10261 cgatttattt ggtatatata tactaagttt agtgatatgt ttgataatat tcataatata
    10321 tggagcagta tctgttaata agtttaagag tgtagtgtat aaaaattagt tgatatatta
    10381 ataaagagaa aggagattct tgtaataaga gtctccttgt tgattaacta aataatttgg
    10441 gtaataaatt catataaaat atgttatgat attaataatt catagcatag tgaaatatta
    10501 ggataatgga gaatatacaa atgtatagaa ttttattagt agaggacgat attgatttat
    10561 caaaagagat tgcattagca cttgaaaaat ggggatttaa agtagaatta attgatgatt
    10621 ttgaggtagt attagatgaa tttatagata aaaaaccaga tgtagtactt ttagatgtaa
    10681 atttaccatt atataatgga ttttactggt gcgaaaaaat aagagctatt tcaaatgtac
    10741 cattaatatt tttatcatca agagattctg atatggattt aataatgggt ataaataatg
    10801 gagcagatga ttacattacc aaaccatttt ctatagaaat attggttacc aaaataaatg
    10861 gtataataag aagagtctat aattatagtg attctaatag tattttgtat tgtgaagatt
    10921 tgatgtttga tgtggggaaa gggattataa aacataagta taaggataaa agtatagagt
    10981 tgactaaaaa cgaaataaaa attttaacat tattattaaa aaataagaat agggttgtat
    11041 caagggaaag tcttatgatg actctatggg ataatgatga gtttgtgaca gacaatgcac
    11101 ttactgtaaa tatgaataga ttgagaagta aagttaaaga actaggtttt gatgatttta
    11161 taaaaactaa gaaggggata ggatatataa tacaatgcta gtaaaatatt taaaaggtag
    11221 gtctaagtat attgctctac ctttagttgt agttagtata ataaatatct acttatttgc
    11281 tatagatatt tttaaaaata aatattcaga gttaatatat ttagattttt tagtgctgtt
    11341 tataataatg gtattttttg ttatagatta tataaatttt agaaatagtt acacaaattt
    11401 atataattgt atagaaaata gtggtgaaat agacagttat ttagttgatg gacaatcttt
    11461 tgaagaaaat ctaataaaag atattataga aaacttaaaa ataaaaaata atagtgacat
    11521 agagacttat aaacaatcat taaaagaatt ggatgagtac atagctaaat gggttcatga
    11581 aataaaaatt ccaatatctt ctttaagtat aattacagat aggttaagta gcatagaaga
    11641 tagtttggat attaagaatc aggttgcaaa gataaatttt ttagtaaact ctattttgta
    11701 tagtagtaga agcaacacta tgtttgaaga tgtatttata aataaattta atttagaaaa
    11761 attggtaaaa atgtctataa aaaataattc atttttgctt ataaaaaata atgtagaagt
    11821 tagtttgaat gaattagaaa atgatgttta cacagattct aaatgcatgt catatgtttt
    11881 agaccaaatt attaataatg ctataaagta ttcaaaggaa gttggtaaga ttgaatttaa
    11941 ttcaaaaaag ttagaaaatg gagtggtatt atctataaaa gattttggta taggtataaa
    12001 tgaggaagat atatctaggg tttttgacaa gggatttacg gggaaaaatg gaagaaatca
    12061 attgtataag tctactggaa tggggatgta ttttgtaaag aaaatgatag atagtttagg
    12121 gcatgagatt gaggtatgtt cggaaaatgg ttcatataca atttttaata tctattttta
    12181 tgatatatca gattatttaa gcctagatag ttaggttatt agactataaa atctatcatc
    12241 atatacaaat cgacatatac aataagaata aatttagttc ttgaaaatag tgaagattta
    12301 attagtgtct taaatgatgc tctatctata ttataaaata ataattatta tatatgaaaa
    12361 acatatcaga taggtacctg gtatgttttt tatttgtagt aactatttta atatggtttt
    12421 tggagagaga atatgttttg tttgtgaaaa aaaattacat ggtattacat tgacactgaa
    12481 aataaattaa tgtaagattt agctaaggta aaaaacgtgt ataagattaa ctcgattaat
    12541 ttcgtaagag taaattatcg taattaaaga tgaaataaga ttaattctaa aattattaaa
    12601 cttaatacct tgatttgggg aggaataact ataaacttaa aaggatgata actatgtata
    12661 gtgtaggttt aatgtcagga acttccttag atggagttga tgcagtacta gctgaaatta
    12721 gtgagaatgg tagaaatact aaagttaagc aaatagaatt tataactcta gaaattccta
    12781 aagatataaa agacgaaata agaaaatgct gtatagaaga ggaatcgagt gttgatttaa
    12841 tctgtagctt aaattttaag cttggatatt tatttagtaa agctgtaaaa tcagtgtgtc
    12901 ataaagcaaa ctttcatata gcaaatttgg attttatcgc atctcatggt caaacaatct
    12961 ttcatatacc aagaagttat aataattttg tatcttcaac gcttcaaata ggtgaaccag
    13021 cagttatagc atatgaaaca aatacaaagg taatatctaa ttttagagta atggatatag
    13081 cagcaggtgg agaaggtgct ccattagtac catatagtga atttttatta tatagtgata
    13141 acaataaaaa tttagcactt caaaatattg gtggaatagg aaatattact ataataccta
    13201 aatcatgtaa catagatgat gtatttgcat ttgatacagg accaggaaat atgataatag
    13261 atggagtatg tcaaagatta tttaatagaa agtatgataa aaatggatac tttgcatcaa
    13321 aaggtaaaat taatgaagaa atgttaaaag atttgatgag tcataagtat cttagtcaag
    13381 cgccacctaa aagcactgga agagaggtat ttggacaagt ttatttggat aacatgctta
    13441 acaaatataa acatgtagat aaatatgatt tgatagcaac agtaactatg tttacggcaa
    13501 aagctatata ttataattat aaaaacttca tacttccaaa ggttaatgta gatactttat
    13561 taattggagg tggaggagct cataatttaa ctttaatagg atatataaaa gagttattat
    13621 tagaggttga agtattaact caagatgaat atgggtattc ttctgatgca aaggaggctt
    13681 tggcatttgt aatcttgggc aatgaaacat taaataattc tttttctaat gtaatatctg
    13741 caacaggggc taaaaataaa gttattttag ggaatataac acctaaacct tttggaggta
    13801 aatgacatgt ctaataataa aaaaaagaaa agaaagaaaa ataataatta taaaaatgca
    13861 aaaagtacaa aaagtaataa aaatactaag aaacaaatgg agaaaaatca aagaacagat
    13921 tataatattc caactataag ttttaaaagt ggattaatac taatagtttc atctcttgta
    13981 agtactgtag tttttccttt gttattatct atatttggaa tagataatag atttggagtt
    14041 gttataggaa attcattaat tacatctttt gcagttgcat atacaagata ctttgttgaa
    14101 actaagagag gtttttcttt gggatttttt agactgtata ttttatttgc tttatcattt
    14161 gcaataatag gttacttctg gatatataaa ggaatacata tttaaaaaag tattgagact
    14221 attttatgtt aataataaga tagtctcaat acttttttaa gaaaagatat gagtatttta
    14281 agtagtgtgt aattttagac aaaagaatga ttagataaat aaagcaatta aagcaagata
    14341 taaatgcatg aaataaaatt acaatttaaa atgtaaaata tgaaaaaata tatacaaacg
    14401 tattcctgta atcaaaaata catgctataa tgattgaaaa gaaataaaaa aacaggggga
    14461 atcaatatga gagtattatt tgtttgttca tcagggatgt caagtgctat agcagttaat
    14521 gcattacaaa aagaaggagc aaaaaatgga atagatatag atgttttggc tgttggtact
    14581 caagagtttg aggatgaagt taaaaatgga tgggatattg ctatggtagc tccacaagtt
    14641 agacatagat ttgactattt aaaagctttt gctgatgagg ttagtgtacc atgtgcatta
    14701 atacaagcac aagcttatag tccattagga ggaccaaagt tattaaaaca agttcaagag
    14761 ctgttgagta aatagtagtt gcagtgttgt taggttagaa gaaagttgat tttataaaaa
    14821 ttttaatgat aataaaaata aatattaatt aaggagagaa aagatgctag ataaatttac
    14881 agaattttta gacactaaat tatcaacttc tatggccaga ttggcagaac aacgtcactt
    14941 aagagctgta agagatggaa tcgtagcttc attaccactt ataatagttg ggtcattttt
    15001 cttgatatta gcatttcctc cattaccaga ttcatggggg gtaactcagt atctaaagga
    15061 aaatgcagtt acaatattac taccatatag aatgacattt tttattatat cagtatattc
    15121 tacatttggc ataggttata gtttggcaaa gtcatataag ttagatggat tgacaggagg
    15181 tttattagct acagctacat tccttttgac aatagttcct actgcaatag agggtcttac
    15241 ccctgattta ttaaagatag ttgaaaagag tccagaactt ttagcttggt ataattcaat
    15301 tcctcaagga tggcaaatgc caatggctaa tcttggtgga ggttcaatgt ttgttgctat
    15361 tgtaacatct ataataggtg ttgagattta tagatttact gacaaatcta aattcaagat
    15421 aaccatgcca gaagcagttc cagcatcagt cacaaggtct tttgaagctc tgacaccagc
    15481 cgcattaata ataattttaa tggctattgt tacttatttc ctagggtttg attggcatgg
    15541 cttcatagct aaattagtaa ctccattagt ttcagccact gacacattac catcagtaat
    15601 tatactaata tttttaataa cgtttttttg gtcatttgga atacatggtg tatcaataat
    15661 aggttcattg gcaagacctt tatggataca attattagat gctaatacag cttcatatgc
    15721 tgcgggtcaa gcagttccag gtattgctgc tgaacccttt tttcaatggt ttatatggat
    15781 tggaggttct ggagctacaa taggcttagc tttacttctt gcatttagag caaaatcagc
    15841 ttatggaaag tctttaggaa aagcaactgt aataccagca atattcaata taaatgaacc
    15901 aataatattt ggagcgccaa tagtattaaa cccaatatta ataataccat ttgttgtagc
    15961 accaatagta atggcaacag tttcatggtt tgctatgagt gctaatttag tatccagagt
    16021 agtagttact gctccttgga cattaccagg accagtaggg gcatatttag ccactggtgg
    16081 agattggaga gcttcagttc ttaacttatt atgtatagct atatcaataa taatttacta
    16141 tccatttgtc aagatgtacg ataagaggtt gcttcatgaa gaaaaagttg aagagcaaga
    16201 agcttattaa ttaaaaacta tattagatta gtataaattt caaagtataa cagttatgaa
    16261 ataagtaacg aagatatttt gttgattgtt tataaaaaaa gaaaaggtaa acttttcttt
    16321 ttttataaat atatatgaaa ctttttttca tcaaattttt aaaaaagttt cgtgtaattc
    16381 gaaataaatt taaacacata taggatataa ggagatgaca cagatgggaa gattaggaat
    16441 atcaatttat ccagaaaaaa caacatctaa ggaaatatat gattatatag ataaagctgc
    16501 tgaaaatgga tttacacgta tattttcatg tttattatca gttaaagata ctaaagaaaa
    16561 tataattaaa aaatttaaaa gtataaatga atacgctaag tttaaaggtt ttgaggtaat
    16621 acttgatgta aatcccagag tatttggtga attagaaata agctacaaag atttgaattt
    16681 ttttaaaaag acgaaggcag acggaataag gcttgatatg ggatttacag gttctgaaga
    16741 ggcattaatg acttttaatc ctcaaaatct taaaatagaa atcaatatga gtaataatac
    16801 taattatata gatacaataa tggactacca accaaataaa gaaaatttga taggatgtca
    16861 taacttttat ccacatagat atagtggatt aaatttaaaa cactttataa aatgtacaga
    16921 aagatttaat aagtatggat taagaacagc agtttttgta acaagtcaaa atgaaactac
    16981 atttggacca tggcctgtaa ctgatgggct tccaacaatt gagatgcaca gaaatctacc
    17041 tatagatatt caagtaaagc atataatagc tatgaataat ataaatgata taataatatc
    17101 aaattgttat ccaacatatg aggagctaaa ggtattaggt tctatgagaa aagatatggt
    17161 tacatttgat gtagcaatag aggaaaatat acgagaagtt gagaagaaga tactctttga
    17221 agaaatacat tttaatagag gggacatatc tgacagtctg ataaggtcta ctcaatctcg
    17281 tgttaaatat aaaggataca aatttgaact atttaatact cctaaaataa taaaaagagg
    17341 agatatagta attgaaagta gtgaatatgg tcattatgca ggggagttac aaatagcttt
    17401 gactgatatg aaaaacagtg gtaaatcgaa tgtagtagga tatataagaa aagaagaatt
    17461 atttatatta gattatatta aaccatggca aaagtttaat tttaaaccct caacaaaata
    17521 atttattatg aaaatattaa tt
//
LOCUS       DAEORZ010000085        17350 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEORZ010000085 DAEORZ010000000
VERSION     DAEORZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.085"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            33..779
                     /locus_tag="KRH39_002449"
     CDS             33..779
                     /locus_tag="KRH39_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBG8512347.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHVELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            840..1514
                     /locus_tag="KRH39_002450"
     CDS             840..1514
                     /locus_tag="KRH39_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG8512348.1"
                     /translation="MNDELKSILYNKGVDIVRFIDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKAEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLKCMMSCPWSL
                     RYANQK"
     gene            1883..3229
                     /locus_tag="KRH39_002451"
     CDS             1883..3229
                     /locus_tag="KRH39_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG8512349.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMYYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGVNTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNIVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3256..4656
                     /locus_tag="KRH39_002452"
     CDS             3256..4656
                     /locus_tag="KRH39_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512350.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            4866..6302
                     /locus_tag="KRH39_002453"
     CDS             4866..6302
                     /locus_tag="KRH39_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG8512351.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGKETVYYYKHHEPEKTNQIQ"
     gene            6646..7590
                     /locus_tag="KRH39_002454"
     CDS             6646..7590
                     /locus_tag="KRH39_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBG8512352.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGITLEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7759..8691
                     /locus_tag="KRH39_002455"
     CDS             7759..8691
                     /locus_tag="KRH39_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBG8512353.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8796..9263
                     /locus_tag="KRH39_002456"
     CDS             8796..9263
                     /locus_tag="KRH39_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBG8512354.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9507..10361)
                     /locus_tag="KRH39_002457"
     CDS             complement(9507..10361)
                     /locus_tag="KRH39_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar uptake protein"
                     /protein_id="HBG8512355.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSFKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSYIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VLMLSIIK"
     gene            10527..12491
                     /locus_tag="KRH39_002458"
     CDS             10527..12491
                     /locus_tag="KRH39_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8512356.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIRYINLHLVAADQHRDTNELKNTSANPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAINHITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            12491..12952
                     /locus_tag="KRH39_002459"
     CDS             12491..12952
                     /locus_tag="KRH39_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8512357.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            12955..13275
                     /locus_tag="KRH39_002460"
     CDS             12955..13275
                     /locus_tag="KRH39_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG8512358.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13291..14391
                     /locus_tag="KRH39_002461"
     CDS             13291..14391
                     /locus_tag="KRH39_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG8512359.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14511..17111
                     /locus_tag="KRH39_002462"
     CDS             14511..17111
                     /locus_tag="KRH39_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBG8512360.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDENIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6903 a         1785 c         3000 g         5662 t
ORIGIN      
        1 aaaaatgtaa actgtgaaat ggaggtagta acatgaaaat tgaaaatctt tctattgatg
       61 atattaaacg aggctatata tttaatgatg atttaaattg ttatagttgc attaaatgta
      121 caaaaacata tgaggtagga gaaatatata aaataggtaa tagattcttt gaagcttcaa
      181 aagctattga acttcatgtg gaattagaac atagtgatta tttagagcaa ttgcttaata
      241 gtgattcaaa gtacaacaca ttgactgaaa atcaaaaaca attatttcaa ttatttatta
      301 gtgatttacc agataaggag atagcaaaac aggttggagt atccacatct actatacgcc
      361 atcaaaaatt tattttcaga gaaaaagcaa aacaggctaa gctatattta gcactatatg
      421 aatctgtatt tgaagataaa tctaataaaa gcagtactat aataccaatc catgaacatg
      481 ctcgaatggt agatgaaaga tatgtagtaa ctgaagaaga gagaaaacac attttggata
      541 catcatttga aagtatgaat cctttaaaat taaaagtatt ttcttcaaaa gaaaagaaaa
      601 aggtcgtaat tttatctaag attttggaac aatttgaacg tgggaagaaa tattcagaaa
      661 aggaagttaa tcagatttta gaatctatat atgatgattt tgcaacaatt agaagatatc
      721 taattgagta tggttttatg agtagaaata aagaatgtac aaaatattgg ttaacataat
      781 taaaataata aattttacta atgtgtaatg ttatttttat ctaggggaag gagtcaaata
      841 tgaatgatga attaaaatct attttataca ataaaggtgt agacattgtt cgctttattg
      901 atatatctga atttccaata aatcaaacac aaggtttttc taaagcaatt ttattttgta
      961 tagggttatc aaaaaaattt attacagata tatacaataa cttacctaca gatagtgatg
     1021 agtttttaga aaaagaggag aaagcagaag aattagctga ctggatttca aagtatattc
     1081 aaaataaagg atatagagcg tattctcaat cggagaaaaa caatctagaa catggatatt
     1141 ttgaaaaagc ctatattaat ccagaaatgc aatcagggat tagtccttta ccacataaga
     1201 caatagcaaa tattagtggc attggattta tgggaaaaaa taatttgttt gtaacagagg
     1261 aatatggatg tgcttttagc atgtgtacag tacttacaga tgctccaatt tctgttgaaa
     1321 gatatccttt gattgattca aaatgtatgg attgtaatgt ttgtgtagaa aactgccctg
     1381 caaaagctat acatggaaat gagtggactt tgccaggaaa aagagagtct attattgatg
     1441 tttcaaagtg tttttgtgta ttgaagtgta tgatgagttg cccatggtca ttaagatatg
     1501 caaatcaaaa ataactttac tagagaaggt attaaattga atattataaa atttttaaaa
     1561 tgtaagatta tataagattt ctcaaaataa aaatttgtat agaacactca gaaatgagtg
     1621 tttttctatt gggattaaat tatattgata caaagcttaa ctattttaat cattatataa
     1681 aatttgtatt aaaaaattaa gaataacttg gtaatagtga aaatataatg agattataat
     1741 tgatttataa tataactata atgatattgt aaaaatgaaa attatcctct caaaattccc
     1801 aaagagagta aaagtcactt cagttatagt caaaaaaatt tttctgtgaa aagaatttag
     1861 atttgaaaag gaggaattaa tcatggaaat tgaaactaaa actccagatt ggattaatgt
     1921 actagaatta aacagtaaaa tagatattac tgggaaatat gatgtaagta atatgataca
     1981 agatattgtc tctgataagt cactagaagg ttccattata tattttccaa aaggtaatta
     2041 tttatttgag aaaggtatta aaattagtca acaaataaca cttcaaggag attcttatta
     2101 tggaggagat aatcaagtat caaatttaga taaaaaacaa tcaatcatag gaacaaccaa
     2161 ttttataact cgaggagttt ctaatatgag tattattgca ttgacaggaa caagtcaatg
     2221 tatcaaaaat attaattttt attatgatag tcatgatata gagaaaatac cgcctaaaaa
     2281 tgtatctgct attacagaat atggagaaac acaaggacta tctcattttg aacacctgtt
     2341 tatttctgga ttttcaggga tagggattga aattccatac tattcaactg gcaatgatat
     2401 aattgttagt tcatgtggtc ttggtatgag gcttggagag aagtcaatgc tttctagtag
     2461 caagatttac gaatgtaaaa atggaatgga gattactact ggtgttagct taaataatgt
     2521 gagaatagaa gaaataaaag aaataggtat taataataaa ggatttggtt ttaatctgat
     2581 tatgaatctt atggtcgacc aatgtggata ttgtggattt atgtttgaaa aaatgtacta
     2641 tagtcagata actgccagaa ttactagatg cggtcagtat tataaaagtg ttgattataa
     2701 tgcttatgag aatatggaag atagaaaaga agaagcttat tctatttttt atggagatat
     2761 tttagaaaat tgcaatattg tattgatgaa tagtaatatt gataccttgg aaaatgattc
     2821 acttaatgaa cataagatat atgtaatcaa ggcaaatgaa acaaacaatg taatgctaac
     2881 atgttatgct gaagcagata actttataca atgtgcaaaa ggtaacttgc tgttagaaaa
     2941 tggtgtaaat acttataaat tttataatgg tgagatttgt tctgtaggag gtgttcaaat
     3001 ttctgatata gatgaaaaag atttaatcaa aataatcgag aacactctgt atatagatga
     3061 taaaggtaag tatttaatgg ttcctagatt aaaaaagaat attgtaatat attcagtact
     3121 agattctaca gaagaaataa ataaattaca tggtggaata tgggaacaga ttgatgtaaa
     3181 agtattagct ggggaatcca tgtactatta taaaaagata aaagattaaa ttatcaaact
     3241 aggaggtagt gattaatgga agagtctata ataattaaag atataagtgg ctggattaat
     3301 gtacttgatt atggaatttg taatgatgaa acaaaagatg taagttttaa gttgcaagag
     3361 attatggatg ctgtaccaga agattctatt atatattttc caaaaggaaa atacttattt
     3421 gaaagtggtg ttaagattaa gaaacgatta accttatgtg gagattctta tattttagcg
     3481 aatgttccta aaaatactgc tggagtaaca cagtttactt ttaaaggaaa gaaaaatcct
     3541 aaaagtgaaa atatagcaat cataacagca aaaagtgtaa gacactgtat taagaacatt
     3601 tgcttttatt cagatagttg tgatagagat atgacatcta atcatgaacc tccaacagat
     3661 ggaaaaccta gatttcatca taatgtaatt ataaattata gaaatatatc tgctgttata
     3721 tgtgaagata aacagggaga accaggtcat tatgaaaatc tatcttttag tggtttttct
     3781 ggaattgcac ttgatatgcc taataattct acagcaaatg atattacaat ttttacatcc
     3841 ggacttggaa ttagtacagg ggaaaattca acaattgcta acagtaaggt ttggggctgt
     3901 gttgatggaa tgataatttc tactggaact tttataaatg gtatgagggt agaagaaata
     3961 aaaaaagttg gaattaaaaa tattggtaga ggtttaaatt ttattacaaa tatcacaatt
     4021 gaccaatgtg gatattgtgg agtttggttt gattctatat ccaatgttca gatatcagga
     4081 aatatcacaa ggtgtgggca gtactattat gatgtaagct atgataatta tttaatgttg
     4141 gataatagag ttaaagaagc atattcttta ttctatggta atttattgga gtcaagtaat
     4201 atagttttat caaataccaa taaagataat tgggaagatt tcaaagaaga taagcataag
     4261 gtgtatcttg tagaagcact tgaaaccaag aatgtactat taacatgcaa tatagatgct
     4321 actgactgga ttgttaagtc agaaaaagga aatttaacgt acaataatat gcgacaaact
     4381 gttatatttt acgatggaaa actaagtagt atcaatggaa ttggtatatc tgatgaagat
     4441 tatggggata aagtcaagat aaaaaaagga aatatatata taaatagtaa ggataaggat
     4501 attaagatgc ataaaccaga ggtaggtgac attatgtcaa caattattaa agataaaagt
     4561 attctttctg ctcattatgg gggaacatgg gagaaaattg gtgaaaaaat agagtttgga
     4621 ataactgtac actattataa attgatagag aaataagtaa atacgtagta tttgaataga
     4681 tgtattttat tggagagcta ggactatagt tttaatgtaa atggtttggt aatagcatgg
     4741 attgaataat ataaaaataa tataaaaaat taatttgatt atgtaaaatt ctatgaagta
     4801 gtaaaactgt gactttagtc ataggaggtt caaactaagt aaaagggaaa ggaatatata
     4861 atattatgga aggaagaact gggattaaag atggaagatg gattaatgta gtagaaagag
     4921 gtattaaaaa tgatggttcg tacaacgtag gtcctgcttt gcaggatatt attgatgaaa
     4981 tagaaacaga tttttgtgtc ttatattttc caaaaggtaa atacctattt acaagcggtg
     5041 ttaaaattaa taaagaaatt gtattgcaag gtgattctaa ttcatctaca ggcactacac
     5101 aatttataac tcgtggagta gaaaatatga gtattattac attgacaggg aaaaaacaat
     5161 gtattaaaaa tataaatttt tattctgata gttgtgagat acaggtaaat aaagaaccac
     5221 cgacaaaagg taatcctaaa tatcattatg agatgattat taataagaat gaaaagaatg
     5281 ttgagatgaa taatgtatca gcaatcctgt atgataacag taagaatatg attaaaggtc
     5341 taggtcatta tgaaaatatt tatataagtg gtttttcggg tacaggaatt aggataccat
     5401 attactcaat tgtaaatgat attacagttt catcttgtgg gctgggtatt gatacaggaa
     5461 tagacacaat tatttctaat agtagaatat ctaagtgtca aaatggaatg aggattagaa
     5521 ctggaactag tataaataat gtaaggattg aaaaaataca gaaagttggt cttataagta
     5581 gtcaagttaa ggggtatgag gggtatggaa gttataaaat taataatatt acgattgacc
     5641 aatgtgggta ttgtggcttt tcatttgatt caatgaaaga tagttatttt tctggtatta
     5701 taagaaggtg tggtcagtat tattataata cagattatga cacatattta agtattaaag
     5761 atagagtaga agaagcatat tctttatttt atggaaatta ttttacagaa tgtaatattg
     5821 atttagttag tgataataag gataactggg atgatggctt agaatataaa cataaagttt
     5881 atgtatttaa aacccttcaa ataccaaaat taacattgag atgcaatata gatgcctatg
     5941 attatatcgt taaatcggta catggaggag gtaatttaat gttaaaaaat aactttaaca
     6001 attatatatt tattgattca tctagtccag aagttattaa tggaattaat tttaattatc
     6061 aaaagaatgg aaacctgtta aggttaaata atggtaaatt aagttataaa aatggaaagg
     6121 aagaaacagc tatattcaga cctaattatg gtcttattgt agcatcaaca gattcagaat
     6181 gtgaacaaat agaggctacc tatggagaaa aatgggagaa gcttggttct gaagtaattg
     6241 gaaaagaaac ggtatattat tataaacatc atgaaccaga aaaaacaaac caaatacaat
     6301 aaaatttgta ttatataatg atataaagtt ttaatatata gtgtttacct ttaatgtaat
     6361 attgatatat ttgtatagta ttaaatttgt taaaaagtaa aattgtaggg tgcttgaaaa
     6421 tagcaccttt tttagtttca ttataaaatt agttttgaaa gtttaggaat aatgtatatt
     6481 tatttttaaa gaattataac tttattaagg atattttaaa tgctagaaat gaatcagtaa
     6541 atcatactgg aattatctta aatgttttga ttaaattaag tatttagagc tataattata
     6601 ttaaaaatat attagaacat aattatatta ctatggaggt taaatatgat agtaagtgtg
     6661 agtagaagaa ctgatatacc tgctttctat agtaagtggt tttttaacag acttaaagaa
     6721 ggttttgttt atgtaattaa tccaatgaat ccaaaacagg ttagtaagat agagctaaat
     6781 ccacatactg ttgattgttt tgtattttgg acaaaggatg taactccaat gttatatgat
     6841 ttaaataaat taaaagacta taaatattat tttcactata ctataacatc atatggaaaa
     6901 gaagtagaga catgtatttt agataaaaga aaggtaatag attcatttaa ggaattgtct
     6961 agaaaaatag gcaaagaaag agtaatttta agatacgacc cgattttctt aagtgaaaag
     7021 tacacagtag attatcatat caaagcattt gcgagtttat gtaatcagct tgatggattt
     7081 acagaaaaat gtataataag ttttatcgat ttatataaga aaacaaaatt taatactaaa
     7141 gcattacata taaagccaat agaaattaca gaaatagaaa cattatcaaa agagatttct
     7201 aagattgctc ataagcatgg gataacactt gaaatgtgtt ctgaagaata taatttaagt
     7261 aaatttggga ttggaaaaag taagtgtatt gatgatagat taatatcaaa aattatagga
     7321 tgtgaagtag aggtaaaaaa agatagtaca caaagagata tttgtggatg tgttaaaagt
     7381 gtcgatatag gacaatacaa tacttgcaga cattattgtc tgtattgtta tgctaacttt
     7441 aattatgcaa aagtagaaga aaactgtagg ctttataaag aagataataa attactcata
     7501 gatactttaa gagatgatgc aaaaataagc attagagata tgaagactat taaagtagat
     7561 aagaataaac aaattagtat atttgattaa aataaatata ctaattacct taatatatta
     7621 agaatataga tacttttgat aacaaaatta aacttaatag atattttcta tataagaaag
     7681 atggtcataa tatgtcaatt tgaccatctt tttgatataa ttaaaacaca aataatttag
     7741 taacaaagga ggcagaactt gaatttatta aaaaaatatc ctatacctat tgcaagtttg
     7801 attttgtcaa tctttacttt aggtaactta ttacaatctt atagtgaatc attacgtaat
     7861 gtaattggat ttataggatt tattttgtat gcaatttata tagtcaaaat aatattactt
     7921 agaaagggtg ttaaagagca attagagaac cctttaatat caagtacatt tccaactata
     7981 acaatggcaa caatgatatt tgctacatat ataaaaccat tatctttaga attaagtatt
     8041 atgatttgga gcataggaat tgtgggtcat cttgtgttaa tagtcttatt tagtaagaag
     8101 ttcttagtta aattctcaat aaaaacggta tttccatctt ggtatatagt atatgttggg
     8161 atagttgcag ctagtgttac ggcaccagca tttaatcaac ttttgattgg caaaattgct
     8221 ttttggatag gatttgtatc ttatatagtt cttataccag taattttaaa gagagtatgg
     8281 ataatcaagg aaatgcctga accatcttta ccaagtatag ctatattagc tgcaccaggg
     8341 tcactattat tgtctggata tattaattct ttttcagata aaaataatgt agtattatac
     8401 ttgttattca tattatcaat tgtattttat attatagttt tgggatattt accgaaatta
     8461 ttaagattgc ctttttatcc gagttttgct gcatttacat ttcctatggc aataagtgca
     8521 atgggtatga aactaatgaa tggttattta actaaatcag gtcattttat aagttggatt
     8581 tcatatgctt ctaagttaga agagataatt gctacagtga ttattttata tgttttagtt
     8641 ttgtttatta tatatcttag taaatctgaa actacatcta aagctaaata agaattatta
     8701 aatatgtgta tttaatagga atattgatat aaatatatga agataagata aaccatagaa
     8761 acttaattta gcttaatttt attggaggaa aacaaatgaa aacaaaagaa gaatatttaa
     8821 aagaagttag aaacatatca gaagaatact ttagaaaagg agagtttttt tgctctgaag
     8881 cagttctcca gacaataaat gatgctttag gccaaccttt aagtccagag ataactaagt
     8941 tagcatcagg atttccgata ggacttggaa aagcacaatg tctttgtgga gcaatatctg
     9001 ggggagagat ggctttaggt atagtatatg gaagagttca tggtgaagct atgaatccaa
     9061 agatgtttga gcatgctaaa ggtttgcatg attatattaa aaaagagtat ggtgcaacat
     9121 gttgtagagc tattacaaag aaatgggatg gtgataactt tatgtctccc gaaagaaaag
     9181 agcactgtat taaaataaca ggtcaagtta cagagtgggt tgcagataag cttatagaag
     9241 atgaaaaact taatatcaaa tagtgtaata ttagttggtg aatttataaa gttaaattta
     9301 cttatagtat aagatacttg atattctcgt attgatagaa aaatttatag catatgtatt
     9361 taatgaaata tacttatatt tagagtgtgg tttaatttca taaatacata tgctattatt
     9421 ttaagtatat aagcaaattt atttaaataa atagaaacag ttaatttata tgtacaaaac
     9481 tatatatgat tagtgtaaat aaatacctat tttataatac tcaacataag gacaccagac
     9541 atgattagta ttataccaat aaaaatagca agccattctt ttttgttctt cttttcttta
     9601 aaaaatataa tccctcctaa agtggctact atcacacaac tctgagagat agaaaaactt
     9661 attgtaactc ccaatgtatt agtcgttatg aacataccta aatttgctat tgaccacaat
     9721 ataccagtaa ttaaattgaa taaaacattt tccctatata caatctcttt agaaaaatac
     9781 ttgacaataa tatatgaagt tatcaacata ccaacagatt gaggtaatat aattctatat
     9841 ccttcaatta aaaatatttg attagttaca acatataatg ataaaaatag agatgataat
     9901 aatacaactc caacacattt taacatattt ttatctgtag attttttagt ttgataattt
     9961 gtgagaagta taccaagtat tatacataat attgataaag aaccaatcag taacatttta
    10021 aaactggtcc attcattaaa caagattaca gctaataatg tcgtaaaagt taattgtgaa
    10081 ccatttgaaa ttggcattac tttagataca ctagcaattt gaattgcttt aaactgcatt
    10141 aattgtccca aactccagaa tatgccagaa atcatactta tacaaaatga aaaaaatgta
    10201 atttcaggat gtataaccag agttattata gtggaaaata taaacgcact tatggatgta
    10261 cctaaaagct gttcatgagg agaacttttc tttagatttg caactatggg catcaatccc
    10321 catcccatta caggaagtaa agcaataatt attgtcagca ttaaactatt tcctttcata
    10381 ttaaatttag acagctttta atttatcatc taaaattaaa gtgttcaata cataaaaatt
    10441 accgtattta atcaggaaat attagtagtt ttttagaaaa acattttcca ttaaaataaa
    10501 gacaaaggga aggagtaaaa ttgaaaatgt taactgatat gattcaaaat cgtgaaatta
    10561 aaataataaa gtttttatta aaagaaggtc atacaactgt aaaagcaata gcagatgatt
    10621 tagatgtttc agaaaaaaca gtttcaaaat ctcttaaaga agtagaagca gtgcttaaaa
    10681 agataggtct ttctttaata agaaaaccta agataggtgt ttatattgat ggagatttaa
    10741 aaaaaatact tccttaccta gataataaag gaagtcaaat tcctacaacc agagaggaga
    10801 gagttatcta tatatattca gtacttttaa agagtaatga ttatattact atacaacaac
    10861 tttctgatga actatatata agtcgtggaa ctattgaaag ggatttagca gaagttgaaa
    10921 aactgctaaa aaacgaaggg atttccatat ataagaaacc tagtaaggga ataaagttaa
    10981 acataagtga aagggataaa agacaactta ctgctaagtt tatacataaa ttttggagta
    11041 gaaactggta tattaagcaa gagcaagaaa gcttttatca agcatttgaa aagatacagg
    11101 cagatgtaaa tggtattttt tcggaagatg atttatcaat aattattgat attcttagga
    11161 atttcagcag agaaagaaaa tttgagttct ctgattatga atttcagtca ttagttattc
    11221 atttggcaat agctgtagag agaattagaa aaggcgaata tatagaagaa agtttgtata
    11281 ttggtaatat agaaaataat caaatgtcaa ataccaatta tttagcaaaa caattagaag
    11341 aaaaattttc aatattatta ccaaaatctg aaattagata tataaattta catttagtag
    11401 cggctgacca acatagagat actaatgaac taaaaaatac ttcagctaat ccttgtgaga
    11461 caagtacaaa taatatatca aatcttcggg aaattataaa aaatacattg tatgagacag
    11521 cttatgacaa ggatttaatt gatggattga cttcacatgt acattcatca ataaatagac
    11581 tgaaatatgg acttagtatt aaaaatccat atgtaaatac aatcaaacaa aacttttctt
    11641 tatcatttga gcaagcttta gatttaaaga aagtaattga atttatatat gatattagga
    11701 taaatgatga tgaatgtgct tatatagcat tacattttga agcttttaga gaaagaatga
    11761 aagaacttcc acaaaatatt agagtaattc tagtatgcag tacaggatta ggttcatcta
    11821 ggttattgtc tgcaagaata aaaaaatact ttccaatgat aaagattgaa aaagtacttt
    11881 ccgtacaaga attagtatcc agtaaaattg atgcagattt agtaataagt actatttatt
    11941 tagagttaga aacaattcca accatagtag ttagtcctat tttaaatgaa agtgatataa
    12001 gaaccttgga aagaaatata caaaatgttt caagttgtac ttctaagaag tataaatctt
    12061 tcagaaattt aatctttgaa gaaaatatat tgttgaatat tgaagcttca aattatgaag
    12121 atgcaattaa tcatataaca gataacctaa taaaaaaagg ttttgcaaaa gaaggtatag
    12181 ctaaaagtgc aattgagaga gaaaatttat cttacacgtc tttccagtca atagctacac
    12241 ctcatgctaa tcctagttat attaaaaaat ctaatatatc tgttgcaacg ttaaaaaatc
    12301 ctattacatg gggaaatgaa aaggtggaag ttattttctt tatttcatta gaaaatgaca
    12361 aaaacctaga gcttgatgaa atatatgaag ttttttatga cttcattgac aataaaaaaa
    12421 atataaatga catattaagt gcaacttcca caaaggaagt gtacgatttg ctagtggagg
    12481 agagtatata gtgaaaatat cagatgtatt gaaaaaagag caaattgttt ttaatcttga
    12541 tacaaataca aaggaagaaa caataaaaaa attaagtagt atatttgttg aaaatgggat
    12601 tgtggacaat gaagaagaat atgttaagtc tgttttggaa agagaagaac attcaactac
    12661 aggtattggt aatggtattg ctattcctca tgggaaaagt aatgctgtca aaaaatcttc
    12721 tataatattt gcaaagacta agcataagat tgaatgggat gctttagatg aaaaaccagt
    12781 agatagtatt ttcttattgg ctataagcaa tattgataag gatagcaatc atcttgtact
    12841 attatctaaa ttagcaacta aacttatgga tgatgacaat gtagatgcac taaaattagc
    12901 caattcagaa caagaaataa tcaatatatt tagtgaagaa ggagagatgt agaaatgaaa
    12961 agaaaaatta tagcagttac agcatgtgca acaggagttg cacatacata tatggcagca
    13021 caagcattaa agaaagcttc aaaaaagatg ggtcacctta taaaagttga aacacaagga
    13081 gctacaggaa tcgataatga gttaactaca aaggatgcac aaattgcaga agtagtaata
    13141 tttgcagtag acactaaagt cagaaatgct gaacgttttg agggaaaaga aatactagaa
    13201 gtaccagtta atgcacctat aaaggatgca gagaaagtaa taaatgatgc attaaagtta
    13261 attgacacta aataaatgga gggaaatgta atgttgaaaa agatagtctc tgagttaaaa
    13321 aagcatgtct taactggaat ctcatatatg attccattag taattgcagg tgctatgata
    13381 atggcgatat ctcgtgtagg tggttctttc tataatatac cagatatatg ggatgctaaa
    13441 tatgcagaaa gtgctagtag tattgttaga ttattacatg atttagatgg gtttggaggt
    13501 actgcgttag gattaatgtt tcctgtaatt gctgcattta taggattttc tatagctgat
    13561 aaattagcta ttgtaccagg tcttgttgga ggtatgattg ctaaagatat aggtgcagga
    13621 tttttaggag ctttggcagt tggtcttata gcaggatata catgtttgtt tattaaaaat
    13681 catgttaaat taccaaaatc agcagcttca atagtaccaa tatttatagt accagtattt
    13741 gggacattaa tcactgttgt attaatcaat tatgtgatag gtataccttt tgccacatta
    13801 aatactggat tagaaaattg gttaaatgga ttatctggta caaatcagat actaatggca
    13861 gctattatag gggctatgat ggcagttgac ttaggtggac cagttaataa agcagcttta
    13921 acaacatcac ttgcattatt aacaagtgga atttatgcgc ctaacactgc tgcaatggta
    13981 ggaatagtta taccaccact aggattggga cttgctacta tactagcaaa acaaaaatat
    14041 aataaacagt tacgtgaagc tgggaaatca tctcttataa tgggtttaat tggagtaagt
    14101 gaaggagcaa ttccttttgc agtagaatct ccattaaaag taataccttc atgtgtaatt
    14161 ggaacagcaa ttgcttcagc tatggcagtt ggtttaggtt cagtaaatgc aactccaatt
    14221 agtggatttt atggatggtt tacagtagag aattggccaa tgtatgtgtt atcaatagca
    14281 gttggaacag taattgtagc tggattagtt atagtattgc gtggaaatcc agtgattgag
    14341 gatgatgaat ttgaagatga tgattttgat gaagcagagt gggaatctta aaatttaata
    14401 actatttaag acgtgttata agctatattc aatatgaatc ttatactaaa taaagtgtta
    14461 aatattcagt ttagacttaa atataaaaga taaagaacag aggagaataa atgataaaag
    14521 cacatattgt taatcataca cactgggata gagaatggta ttttacttct ggagatgcat
    14581 tagtacttag tgaacaacta tttacagatg taattgatga attagaacgt aatccagatg
    14641 taagttttgt acttgatgga caactttcta ttttggatga ttatgtacaa ttacacaaag
    14701 aaaaaataga aatcattaaa aagttaataa aagaagatag actacatatt ggaccatggt
    14761 ttactcaaac tgatgcattc tttgctagag gagagtctat tttaagaaac ttaatgattg
    14821 gaatttttga aagtaaaaag tatggtaagt atatgaaaat tgggtatttg ccagatacat
    14881 ttggatttaa tgcacagatg cctatactat taaagcatgt aggtcttgat aatatattct
    14941 tttggagagg tatacatctt ggtaaacagg ttcaatcacc atattttaag tggaaaagct
    15001 taaatggaga aagttatata tatgcagtaa atatgccaca tggatatgga actggtatgc
    15061 ttttagagcc ttcattaaag tatgttaatg gacgcttaga cccagctatt gattttatta
    15121 aatcatatac tgatgtagat gaggtgttaa taccttctgg gaatgaccag ttaaatataa
    15181 taggtgattt caaaaataaa attaaagcga ttaatgatat cggaaagtat tcatatataa
    15241 cgagttcata tcaagagttt ttaaaatata taaagtccat agaatcacta gaaagctata
    15301 gaggtgaatt tagagaacct gtattagcaa gaatacataa atcaattggt tctagtagaa
    15361 tggatataaa attagcatgt gataaactag aaaacaagct gattaaaaga atagagccat
    15421 tattagtaat tgctaaaaag agcaaaattg aaataagcaa tcagttactt ataaatacat
    15481 ggaaaaaact tttggaagga caagcacacg atagtttagc tggatgtgta actgatactg
    15541 taacagatga tattcttcac cgtataagag aagcaaatga aatatgtgac agtattgaaa
    15601 atactattgt aaaaaagatt tcagaagggc ttaaattatc taaaaatgat attttagtat
    15661 ttaatacaga agctaagcgt tttaatggat ataaagagat acaagttgta agtgatagta
    15721 aaaatattta ttttaaagat aatttagatg ctactataat tgaagaaact tatgtaaaac
    15781 cgagagaaaa tgtattagaa gaaacacctg ctggtaatat atttattgaa gaaccaggat
    15841 attatatatt aaaagtaaga ataaatgtac agcttcctgc acttggttat aaagttgtta
    15901 gttttgaatt aagtgataaa gaaatggtgt cacttgataa atctaatgac acttttatat
    15961 ctaatgataa ttataagata atatatgaag atggaagctt aaatttacaa cttaaagatg
    16021 gaacttacat atcaaacttt ttaacattaa aagatagtgg taatgcaggt gatacctatg
    16081 acttttctcc actaaaaaat gatgagaata taaaactttc gtttgataaa gtgtatacag
    16141 aaaaagcttt aggatatgaa aaaatggtgg ttaaaggtac tactttacta ccacttacat
    16201 tagaagatag aaaaaacaaa tatcttaatg gtaaattaaa tgtaaaagta agtattgtct
    16261 tatctaaaga aaacccattg atggatgtaa aactttgtgt tgataacacg atatataatc
    16321 atagactacg tgttcatata aaaactgata taaaagataa taaaaacata gcatcattgc
    16381 catttggata tataacaaga aaaaatggag ttttagacaa ctgggaagat atttacagtg
    16441 aaatgcctat agatttagaa cctttggaaa gtaatattac gcttacaaac caaaatagaa
    16501 gttgtactgt atttactaga ggaataaaag agtatcaaca tattgaagat gaaattgcat
    16561 tgaccttact tgctactaca gatgaactag gaaagcctga tttattatat agaccaggac
    16621 gtgcttctgg tgatacaact aaaaaaggtc atataagaat taaaacagat aaagctcaaa
    16681 tgcttaagga attagaattt tcatttgcaa tatatatgga ttctaaatca tttgatgaat
    16741 tggaaattgc gaatttaacc cacaattatc taagagaatg tgtaaattat caattacagg
    16801 attacaattt ctttttatat cgtatagata ataagattca aaaatcaatt gtcaataaaa
    16861 ctgtaagtag agaattagaa ataatatctt taccagaaaa ctatcttata tcagcatgtt
    16921 atccttctta ttacagtaaa gataaattta taataagagt ggaaaatcca agtagtaata
    16981 gaatgatatt ggatgagcaa atatttaaaa atagaaatgg gaaaattgta aatgcaatag
    17041 aagatgtaga agaggaacag gtatatgaaa tagcaccttt tgacgttatt tcaatattat
    17101 tggatttata agtgtaaaag taagtttatt gtttaaagtc tgacagatat taatctgcca
    17161 gactttttaa tttgtataaa tttgggtaaa tattaatgaa tggttttagt atttaagtat
    17221 gaatttagta tttaatatag aatttattga agtagaataa ttagtttaac aggataagca
    17281 gtttaaataa agaattatta tattagagtg attttatata taatttaaaa taataaaatt
    17341 ttaataaaat
//
LOCUS       DAEORZ010000086        17317 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEORZ010000086 DAEORZ010000000
VERSION     DAEORZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17317
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.086"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(292..684)
                     /locus_tag="KRH39_002463"
     CDS             complement(292..684)
                     /locus_tag="KRH39_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8512361.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            1251..1613
                     /locus_tag="KRH39_002464"
     CDS             1251..1613
                     /locus_tag="KRH39_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512362.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1597..2331
                     /locus_tag="KRH39_002465"
     CDS             1597..2331
                     /locus_tag="KRH39_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512363.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDISNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEYEEDEEYEEEL
                     KQNIIENIKKNFDEKNLVQVYKYRGV"
     gene            2351..2770
                     /locus_tag="KRH39_002466"
     CDS             2351..2770
                     /locus_tag="KRH39_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512364.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRQINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYEI"
     gene            2760..3284
                     /locus_tag="KRH39_002467"
     CDS             2760..3284
                     /locus_tag="KRH39_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512365.1"
                     /translation="MKYKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            3596..4153
                     /gene="efp"
                     /locus_tag="KRH39_002468"
     CDS             3596..4153
                     /gene="efp"
                     /locus_tag="KRH39_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBG8512366.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            4245..4700
                     /locus_tag="KRH39_002469"
     CDS             4245..4700
                     /locus_tag="KRH39_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG8512367.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            4863..5573
                     /locus_tag="KRH39_002470"
     CDS             4863..5573
                     /locus_tag="KRH39_002470"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBG8512368.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5627..6697
                     /locus_tag="KRH39_002471"
     CDS             5627..6697
                     /locus_tag="KRH39_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8512369.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6725..10279
                     /gene="smc"
                     /locus_tag="KRH39_002472"
     CDS             6725..10279
                     /gene="smc"
                     /locus_tag="KRH39_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBG8512370.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLESSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSTNLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            10306..11586
                     /gene="ftsY"
                     /locus_tag="KRH39_002473"
     CDS             10306..11586
                     /gene="ftsY"
                     /locus_tag="KRH39_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBG8512371.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11689..12057
                     /locus_tag="KRH39_002474"
     CDS             11689..12057
                     /locus_tag="KRH39_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBG8512372.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            12054..13406
                     /gene="ffh"
                     /locus_tag="KRH39_002475"
     CDS             12054..13406
                     /gene="ffh"
                     /locus_tag="KRH39_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBG8512373.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13444..13716
                     /gene="rpsP"
                     /locus_tag="KRH39_002476"
     CDS             13444..13716
                     /gene="rpsP"
                     /locus_tag="KRH39_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBG8512374.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13781..14008
                     /locus_tag="KRH39_002477"
     CDS             13781..14008
                     /locus_tag="KRH39_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBG8512375.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            14116..14631
                     /gene="rimM"
                     /locus_tag="KRH39_002478"
     CDS             14116..14631
                     /gene="rimM"
                     /locus_tag="KRH39_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBG8512376.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14640..15335
                     /gene="trmD"
                     /locus_tag="KRH39_002479"
     CDS             14640..15335
                     /gene="trmD"
                     /locus_tag="KRH39_002479"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBG8512377.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15520..15870
                     /gene="rplS"
                     /locus_tag="KRH39_002480"
     CDS             15520..15870
                     /gene="rplS"
                     /locus_tag="KRH39_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBG8512378.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            16191..17087
                     /gene="ylqF"
                     /locus_tag="KRH39_002481"
     CDS             16191..17087
                     /gene="ylqF"
                     /locus_tag="KRH39_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBG8512379.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
BASE COUNT         7455 a         1569 c         3197 g         5096 t
ORIGIN      
        1 ctataaagtg acaaaataaa aaatactatt taaatagata ttaaaaaatt gataccaagt
       61 atttgtttgc tatatgaata acaatttatt taggaacttt actttaacac tgtataataa
      121 taaagtaatt gctaaaaacg tttgatgatt tggatgtatt tttagaaatt agaaaaatat
      181 atacttactg actaatgata atcttatatg tattttagtt tgagtgattt aagaaaagtg
      241 ttttgaatgg ggattatttt atagagtaaa taaatatata atagtagaaa attacatata
      301 aagagtagtg tttatttcat taccttcttt atctgttccg atagggtctt gtagagatac
      361 ttgagtttta ttttttttgt tagatctaat attcattaaa atctcatttt caatacatct
      421 agaagcatat gttgctaatc gagttccttt atctatgtta taagtttcta tagctttaat
      481 taatcctatt gtacctatgg atattaaatc atcctgatct tccgttgaat tattatattt
      541 ctttgctata taagctacta atctcatgtt tctttcaatt aatgtatctt ttgcactttt
      601 atcgttttct attttaaatt tagttaaata ctcaatctct tcttcagggg ttaagggttt
      661 ttcaaaagat ttaagagctg ccaataggtc accccctttt caacggtcac tttaaattat
      721 atgaaggaga cttatccaag ttgactgtta tctaataaaa taatatgcat atttttagaa
      781 tttaaaacat atatttgcaa acttttgaat ggaatatgac ataaagagtg ggaatttaaa
      841 tttaagtttt tgttgtatat atatgaaaca aggcgaagaa attattgatt tataaagtat
      901 aaattggaat tgaggtgtca ataaatataa aaggaaatag tatttatata tgttttggtg
      961 ttgaagtaaa agattaatgc aaagcattag agtctataaa aatatgaata taggaggtgt
     1021 aattttacaa tacttaaaaa agtaaatgta attaattgtt tgtataaagt tgattacaag
     1081 caaaagtatc agtcatagtt aatactgtta aggatgaagg tggtgtgtta atttatatat
     1141 gtatgggaag aaattttgat tacaatgaaa aaacttacaa aagatattta cgaaaaaact
     1201 tatagttaaa tgtttatata taaaatatta tttaggggtt gtttttatta ttgaaaataa
     1261 gaaagagtgg ttttatatca attgaatgta taataagtat tgctatatta tatgtggctg
     1321 tttatttagt ttctacatca ttgtataatt gttatagttt tatcagtaga aatatatctg
     1381 acagagaaat gttaagtaca gcaaaaaaat atatagaaga tgagaagtat agaatacaaa
     1441 atagtaagta tgagttaatt gaagataaga tagaaaaaaa ttacataaat ggatatgaaa
     1501 ttaacagtag aatagagcaa attttagatt attatcaatg ctatgaaata aatatagaga
     1561 taaaaaatga atttaaaaaa ctgaggttta atagctatgt tactagaaaa taaaggctct
     1621 atattaataa taacattgat aatattttct attataagta ctgtgtgcat tatgtgtata
     1681 ggtttaattt atacacataa taatatgttt gagttaggat acaaagacat tcaactaaaa
     1741 gaaatttctt taagtggaat agaaatatca gtaagtaatg tactttcttg tgttgaggaa
     1801 gctattgata tatctaataa tgaagacgaa tttagagaat atttcttagg aaataatatg
     1861 attgaatgta taaaaaaaat aaaagataca tcttatagta gtttagaaga actttcttta
     1921 aaagtagaaa ataatactat ttatgacaga ggagattttt ttgaatttga tatagaaagt
     1981 aatgttagag aaaaagaatt tttaaaagca ataaaagtaa gagtgaaaat caaaaatcca
     2041 tggataggaa ttgacattga agattatatg gataaatcta ataacaataa aattgatgac
     2101 aaaacattca ataattataa agcatctgaa aataatgaaa ttctaaatca atttaaaaat
     2161 gaaagtgaaa atataaggaa tcaagaggtt ggtggattta cagaagaaga tgaagaatat
     2221 gaagaagatg aagaatatga agaagaactt aaacaaaaca taattgaaaa tataaagaag
     2281 aattttgatg aaaaaaacct agtacaggta tataagtata ggggtgtata actttgaaaa
     2341 aagaaggaat gtgactgtaa tagaattaat agtatctatc agtataatac tcttgatagt
     2401 tacattatct tttccaaaag ataatcttga aaatcataaa ataaatttat ttgcacgtca
     2461 actttgttca gatataaggt atgttaggca aattaatatg tttagcaact acaatacata
     2521 tatatattat ataaatcaag gtaaacacga aggttatgta ttgagaaaag atggtaaaaa
     2581 cattaaatca ataatgctac ctaaaaatgc aaaaatagct aggagtattg aaataataaa
     2641 gtttaaatct gatgggtcgc ctcaaaaggc gtctacaata gagatatata ataatagatt
     2701 aagaaaaact ataacaataa cacctgtaag tggaagagta cttctgaaag agggaaaata
     2761 tgaaatataa atatggttac ttgcttttgg aaagtgtcgt atcattgtct tcaatggtga
     2821 taattatact agttttatat tctatatttt tgtctacaat aaacttaaag ttaaaagtag
     2881 aagataagat agaactacaa caacaaagtc ttgaaatcat aaaatctatg gaaggaatta
     2941 ttagcaattc tatgggaata atgaatgtat ctaattatga agaaacattt aaaaagacaa
     3001 catctataaa atgtagatat ttagatgaaa ataataatga agaaggcata agtaataaag
     3061 aaattatatt gaatgaaaga cgaaataagt tatttgtaaa ttctttaaat ggcgaaagta
     3121 gtcaagcagg tggttatgaa attggagatt atgtagatga aatgtatgta tcaattacta
     3181 ataatggtca atacgttaat attaaattga aattatcaaa acgtagtcaa aaatatgaga
     3241 ctgatttcaa aataaaagta tggaatttta gtgaaagtat ataaaaatac ataaatttaa
     3301 gagaaagaaa tgacatcaat aagtcatata aaatataatt attgagaact atataactaa
     3361 ataatatcaa tatgtaaagt gtatagatta agaatataga ggataataat aaaaatatag
     3421 aataagtaat ttgccaaata cttgtttata ttgtataact tatgatatta tgatattagt
     3481 aaaaatttgt aatcctgact tgacagaatt caagtaagag atataatttt aaaggcatag
     3541 taatgtaaaa tttactaaaa aataagaaat ttattttaat atggaggaac aaaaaatggt
     3601 atcagcaggt gattttagaa aaggtgttac atttgaaaaa gatggacaac catgtttagt
     3661 agttgatttt caacacgtta agccaggtaa aggagctgct ttcgttagaa ctaaatacaa
     3721 aaacttaaaa acaggagcta taagagaaga aagtttcaat ccaagtgaaa aattccctaa
     3781 agctgttata gatacaagac aaatgcaata tctatacaat gatggtgagt tatattattt
     3841 tatggatcaa gaaaattttg aacaaatacc attaaactat gagcaagttg aagatgcaat
     3901 taagttctta aaagaaaatg aagttgctac aataagattt taccaaggac aaccattcca
     3961 agtggaagcg ccaaactttg cagagctaga agttacagat acagagccag gtataaaagg
     4021 tgatacagca agtaatgtaa ctaaagcagc tacagttgaa actggagcag ttgttcaagt
     4081 tccattattt ataaatactg gagataaagt aaagatagat actagaacag gtgaatattt
     4141 atcaagagta taaaaattta tgtatataaa agcttatatg tcttaataat ataagatata
     4201 tgagctttta gtttatatat aaaaaagaaa gcgaggggtg ttacatgaaa catttatatg
     4261 aagaagttgc atacttaaaa ggcctagcag aaggactaga aataagtgct gaaagtaaag
     4321 aagggaaaat gatacataaa atagttgatg cattagaagt atttgcagat gctattgtaa
     4381 cattggatga agaacaagaa gaacttcaag attttgttga atctatagat gaagatttag
     4441 cagatttaga agaagatatt gctgacatgg aagaagatct ttatgaagaa gatgacgatg
     4501 aggatgatga ggacttcagc tatatagaaa tggaatgccc aaattgtgga gagttagtag
     4561 aaatagacga agatttgtta tatgatgatg aagtagatgt tgtttgtcct gattgtaagg
     4621 ctgtgatatt atcatcagaa gatgattgtg atgatgattg tacatgtggt ggttgttcaa
     4681 gttgtgatga tagagagtaa aaattcataa gaattaaaac tgcctatgat gcctatgaga
     4741 tattttgctt cataggtagt tttattttta tgaggaaggg tattatgtat tttggttttg
     4801 caatacttgg acaaaaatag tacactagaa tttatagaat aggattttgg agggatgtaa
     4861 atatgaaaat aagtaaaaaa ttactagata atatacaaag atttgaaaat gttataaatt
     4921 ataaatttaa aaataaagaa tatatactag aagctcttac tcatagttca tattctaatg
     4981 aaaataaaaa atataatttt aacgaaagat tggaattttt aggggattcg gttttaggca
     5041 tagtgataag tgattattta tttaatgaag aagctaatct gcccgaaggg gaactaacta
     5101 aacttagagc aaatatagtt tgtgaagatt cattaagtga agtagctaat gatataaatt
     5161 taggtattca catgttatta ggtagaggag aagaagcaac aggaggaaga cataggactt
     5221 caatattagc agacgcattt gaagctgtaa tagctgctat atatcttgat ggaggttttg
     5281 aaagtgcaag acagtttata cttcatcata tggaaaatat aatttatgat tcaagaaaag
     5341 gaaatatatt tagagactac aaaacgcatc ttcaagaggt gttacaaggg aatggagaaa
     5401 ataatatctg gtacaggctt atagaagaaa aaggaccaga ccataataaa agatttgtaa
     5461 tggaagttgg aattaatgac gatgtacttg gtataggaga aggcaagagt aaaaaagaag
     5521 ctgaacagtt agctgctaaa atagctttaa agaaaaaatt atgggaaaaa taaatataat
     5581 atgtaaaaat ataaaaagaa atatttatat tagaaggatg ttgtagatga aaaagagaat
     5641 tatacctata tttgttcctc acaaaggatg tcctcatgat tgcatatttt gtaatcaaaa
     5701 aaaaataaca ggtgtgagta ctgatgttac tagtgaagat gcaagaaata taattgagga
     5761 atgtttagag actatagata aagatgcaga tgtagaaata gcattttttg gtggtagctt
     5821 tactgctata gatattgata tacaaaaaag tttattatca gttgctaaag aatatgtaga
     5881 aaaaggtctt gtaaaagata ttagaatgtc tacaagacca gattgtattg atgaagatat
     5941 attaagtatg ttaaaagaat ataagacaag tataattgaa ctaggagtac aatccttaga
     6001 tgaaaaagta ttattagata gtataagagg tcatcaatct gaaattgtat acaagagttc
     6061 aaagatgata aaaaatagtg gaatcaaact tggattacag atgatgattg gtcttccagc
     6121 tgacacagaa gaaaagtgta tatttacagc aaaaaaattt gtagagctaa aaccagattg
     6181 tgttagagtc tatccaactt tggtagtaaa agatacaggt cttgaaaagt taatggaaca
     6241 aaataaatat aatccattta ctcttgaaga gagtgtaaat atagtaaaaa aagttcttgt
     6301 actgttttat gttaataata tcaatgtaat aagagttggg cttcaagcta ctgatgatat
     6361 acaaattgga aaagctgttt tagcaggacc atatcatcct gcatttagag agttggtaga
     6421 agcagatatg ataaaagatt atttagaatt tgttattttg caaaataaaa atataaagca
     6481 aatgttggtc aaatctaata aaaagaatat ttctaaaatc ataggaaata aaaaaacaaa
     6541 tgttaaatat atggaagaaa aatttggtgt gcttttaaaa acacaagaat cagatttaga
     6601 tataaatcaa ttagaaattg tgttagatgg gaaaagttta attagtgcta atatgagaga
     6661 cattcataga aaattgtatg acatttatga cctttagttt taataatgaa gggagagaaa
     6721 agatttgtat ttaaaaagac tagagttaaa gggatttaaa tcctttccag taaaaacaga
     6781 tattattttt aaagaaggaa taacagccat agtaggacca aatgggagtg gaaaaagtaa
     6841 tatatctgat gctgtaagat gggtattagg agaacagagt attaagagtc ttagagggga
     6901 taaactcgaa gatgtaatat ttgcaggtac agatacaaaa aaacctatga attattgtga
     6961 agtggctctt actattgata attctgaaaa tcaattggaa ttagatttta cagaagtaac
     7021 tataagaaga agggcttata gaaatggtga aagtgaattt tttctaaata ataaaagttg
     7081 tagattaaaa gatataaaag aagtcttttt agatactggt attggaaaag atggttattc
     7141 aatcatagaa caaggaaaag tagatgaaat attaagtaat aatcctttaa gcagaagaaa
     7201 agtttttgat gaagcttgtg gcatatcaaa atatagatat aaaaaacagg aagcagaaag
     7261 gaatttaagt aatacaaagg aaaatttaga aagaatagat gatgtatata tagaaattga
     7321 aaatcaatta aagcctcttt ttaatcaaca gacaaaggca aaaaagtact tggaaataag
     7381 tgaaaaatta aagacacttg aagtaaatag ctttataaga gaaattgaag gaatagaaaa
     7441 agaattaagt gaggttaatg aacatcgtaa ggttattgaa aaggagctca atgaaaaaga
     7501 agaacaaaaa aatgttgtag agaaaaagca agaagatatt aataaagagg tagaggtatt
     7561 acaagatgta attgaaaaat ctgtagatta tataaattca attaaaggag ttatatcaaa
     7621 aaaagaatcc caaataaatt taattaagga aagaataaga aattttacga atgaaattag
     7681 tagaaaaaat ttagaaataa aggatataaa agagaagcta aatgaaaata agcaatacat
     7741 aaaagaatta gaaagcaata aattatcagg tagtgaagaa ctatctacct tgcaggaaaa
     7801 tataaaagta ttggaatcaa gtaaagataa acaaaaaatt aagttagaat ccctaaataa
     7861 tgagatagaa ttattgaaag aaagtataat tgatatatta aataagaaac aagagtttag
     7921 taataagctg tccactttaa acgcaaataa agaaaatatg aatatcagag atgaaaatat
     7981 aaattcagag ataacagagt taaacaaaaa tattgaaata aaatcatctg aattagatac
     8041 tataaataaa gaatttaata tgcaaaatga aaatttaaag aatgtaaata atagacataa
     8101 agaattatca actaatttgc aggattcaat tagtgaacac aataaactag aagatgaaat
     8161 acagaaaagt aaatataatt taaatggata taattcaaag ttaaatgtct atatagatat
     8221 ggaaaatcat tatgaaggat ttaatagagg agtaaaagaa gtactaaaaa ataaaaattt
     8281 aaaaggagtt catggagcat taggacagat tataaatgta cctgaaaagt atgagaaatc
     8341 tatagaagca gctcttggtg cttatatgca aaatataata actgataatg agtttagtgc
     8401 taagtctgca ataaactatc taaaacagaa taatttaggt agagtaacat ttcttccatt
     8461 aaatataatt aaatcaaata aaataagtct aggaaattta aaagcaaata ctaagttcat
     8521 aggaatagca agtgatttaa ttacatttga tgaaaagtat agaaatataa ttgaaaatat
     8581 acttggaaga acaatactta taaataatat agatgagggc ataaaatttg caaaagaaac
     8641 tggtcatagg tttaaaatag taactttaga tggtgaaatc ttaaatccag gaggttcttt
     8701 gactggggga agtctaaaaa ctaatgggaa tatactatcg agaaaaagat acattaatga
     8761 atatacagaa aagataagta atatcaagaa tgaaatttca catctagaat taaaaagaga
     8821 atctttagac aaagatgtga aaaatatcaa aaatgaaatt gattcacatg aaagtaaaat
     8881 aaaagattta gaaaaaagta taataataaa aagtacaagt ataaagaatg ttgagtctga
     8941 aattgaatcg ttaaaaggta gtattactaa attagaaaat gagaagaatg atttaaattc
     9001 taacctaaat tatacacttg aaaaaagtga tgatgttaga aaagatatgg aagagttaga
     9061 tgatttatac aataaaaata aagaaaaaat tgatgcattg aatgaggaaa taaaaagata
     9121 caatgattta tatgataaag aaaaatctga atttgatgaa ttgaacttaa gtttagtgaa
     9181 gaaaacagaa gtttacaata gtatagttag agatataaaa agaatatctg gtgaaaattg
     9241 tgagctagaa gaaaaaaata aacaattaga agagtcatta aattatgaag aacatgaaat
     9301 aattaaatta caagattcta tactcactga agaaaaagaa aaagaaaatt taacaaaaca
     9361 actaggggat agtaatagaa atcttgaaac tagaaaaata gcaaaagatg atttaaaaaa
     9421 tagttttgat gaaattaata aagaattaaa aactatagat agacagcata tagaacttaa
     9481 agaaagttta tttaaagttg gtggaagatt agaaagatta aaaactagtc aagacacata
     9541 tataaataag ttgtttgagc aatatgatat gaccttagta caagcattgg aaattaaaga
     9601 tgaagatttg gatatagata gaaaattctt agaaagtcta aagagagaaa taagaagtct
     9661 tggaaatata aatatagact ctataaagga atatgaggaa ataaaagaaa gatacgattt
     9721 ttatagcgaa caaaagcaag atttagaaga gtctatggaa gagatagaaa aacttataca
     9781 tactttagaa gaaaatatga aatctgaatt tgaaattaag tttgaagaaa taagtaaaaa
     9841 ttttaaatat gtatataaga gattatttgg tggaggttgt ggagaattaa ctattttaga
     9901 taaagaaaat ttattagaaa gtgatatact aataacagct caacccccag gtaaaaaaat
     9961 gaaaaattta aacctattat ctggtggaga gaaagcttta acggcaataa gtatattatt
    10021 tgctatattg attacaaagc caacaccatt ttgtatatta gatgagattg aagcgccact
    10081 tgatgacgca aatatattta gatttggaga atttttgaag gatttatcta aagagactca
    10141 atttatatca gtaactcata ggagaggaac tatggaagct gctgattata tctacggagt
    10201 tacaatgcaa gaaaaagcca tttcaaaagt tattagttta aaattaaaag aagctcagga
    10261 aataacagat attatataac ttagattgac ataggaggaa taacagtgtt aaaaaaatta
    10321 tttggttttg gaaaagacaa ggaaaaggaa atagaaaaaa aagatgctga agaagaaata
    10381 gaagttgaag acagtgttga taatttagaa aacttggaag aaactatttt ttcaggactt
    10441 gaagaagaag tgatagataa agtggaagat gttgaagaaa aaaatgagga aagtgtcaat
    10501 aatgaaggca ctgaagaaat cgaaaacttt gaaaaaatag aagttgagat ggatagtaat
    10561 ggaaaagaag tagaaaatat ttcaaataat gattctcaag aagagataat cgaagaatta
    10621 gaaaattatg atgaaattga gtctaaagag atagaagata aagaagataa gaaagttaac
    10681 ttgtttgaaa gactaaaaca aggattgaca aaagcaaagc aaggaataac tgatagaata
    10741 gatgaagttt taaaatcata tacaaagata gatgaagaat tacttgaaga tttagaggaa
    10801 attttaataa cagcagatgt tggtgtaaat acaactatgg atataattga aagactaaga
    10861 gataaaataa agcaaaaagg tattactgaa ccaataaagg taagagaaga acttaaatct
    10921 atagttgaag atattcttac aaatgaaaac tctacactag atatagaacc agcaccatgt
    10981 ataatattaa tggtgggtgt aaatggggtc ggtaagacta ctacaatagg taaactagca
    11041 aatagataca aaaaagatgg taaaaaagtt ttattagcag cagcagatac atttagagca
    11101 gcagcgacag agcaattaga aatttgggca aatagaacca atgtagacat aataaaacat
    11161 caagagggag cagaccctgg agctgtagta tttgatgcta taaaagctgc aaaagctaga
    11221 aaaacagatg tattaatatg tgatacagca ggaagattac acaataaggc taatcttatg
    11281 aatgaacttg gaaaagtatt taaaatagta gatagagaat ttccagaagc aaaacgtgaa
    11341 gtacttcttg tggtggatgc tacaacaggt caaaatgcag ttgttcaagc aaagacattt
    11401 aaagaagttg cagatataac aggtatagta cttacaaaac ttgatggaac tgcaaaaggt
    11461 ggagtagtcc ttgcggtaaa atcagaagta gatgtacctg taaaacttat tggagttgga
    11521 gaaagtgtag aagacctaca agatttcaat gcaaaatcat tttcagatgc attatttggt
    11581 aactaatgtt cttttaaaaa taatggttga caagagtgtt gttatgatat aaaatacaat
    11641 agtgtaaagt aaataacctt tacagtatac tgctaggaag tgtaagatat gaatatagaa
    11701 aaaatggtcg aaattggatt gctatttgaa cagtataaag aattattaac tgacaagcag
    11761 aaagaaatag ttgctctata ctatgaagaa gattattctt taggtgaaat tagtgaaaat
    11821 ctgaatgtat caagacaagg tgtttatgat accttaaagc gttcagaaaa aatactaaga
    11881 gattatgaag aaaagttaca cttagtatca aagattcaag agcaggaaaa aaatattaag
    11941 attataaaag ataaaattat tgacattaaa gaagatttat tgcacaatag agattgtgct
    12001 aatttaatcc ctaagctaga aaatatagaa gatgtatgta gggagatgat aaaatgatat
    12061 ttgaaggatt gtctgataaa ctacaagggg catttagtaa gttaaaatct aaaggaaaac
    12121 ttacagaagt agatgtaaaa aatgctatga gagaagttaa acttgctctt cttgaggcag
    12181 atgttaactt taaagtagtt aaagatttca taaaaaaagt tcaagagaga tgtgttgggc
    12241 aagaagttat gcaaagctta actccagctc agcatgtaat caaaattgta aatgaagagc
    12301 ttacaagttt aatgggagat gtacaaagta aagtaatgat ttcctcaaag cctcctacaa
    12361 ttttaatgat ggttggattg caaggtgcag gtaagactac tacagcagga aaacttggag
    12421 gatattttaa aaagcaaggt aaaaaacctc tattagtagc ttgtgatatt tatagaccgg
    12481 cagctataaa gcaacttcaa gttgttggag aaaaattgga tatacctgta ttcaatatgg
    12541 gagataaaga aaatccagta aatattgcga aagcaggttt aagccatgcg attaaaaatg
    12601 ctaatgattt agttattata gatacagctg gtagacttca tatagatgaa gtacttatgg
    12661 atgagttaaa gtctattaaa tcagaagtta agccacatga gatacttttg gttgtagatt
    12721 ctatgacagg tcaagatgct gtaaatgttg cagaaagctt caatgaggca cttggtgttg
    12781 atggagtagt attaactaaa ttggatggag atactagagg tggtgctgca ctttcaataa
    12841 gagcagttac acaaaagcct ataaaattta tggggatggg agaaaagcta gatgatatag
    12901 aacctttcca cccagataga atggcatcaa gaatattagg tatgggagat gtcttaagtt
    12961 taatagaaaa agcacaagaa aatatagact tagagaaagc taaagaatta gagcaaaaaa
    13021 ttaaaaaaca agagcttgat tttgaagatt tcttacaaca aatggaacaa atacaaaata
    13081 tgggacctct tgacaagata cttggtatga tacctggtat gggaaatgta aaagaccaac
    13141 ttggagatgt agatttgaat ggcaaagaaa tgaagagaac aaaagccata attcagtcaa
    13201 tgactacata tgagagaaga aacccaggag tattaaatgc ttcaagaaag aaaagaatag
    13261 ctaaaggcag tggaactagt gtacaagatg taaatagact tataaaacaa cttaatgaaa
    13321 tgaaaaaaat gatgaagatg tttgctggtt cacaaaagag tatgaagaaa aggggcggct
    13381 taggcggttt accttttttt aaataaaata taaaaaagta aaaattattc ggaggtgact
    13441 gtaatggcag ttaaaataag attaaaaaga atgggagcta acaaaaaacc tttctacaga
    13501 atagtagtag ctgattcaag agctccaaga gatggaaaat ttatagaaga aataggatac
    13561 tataatccaa tttctgaacc taaacaagta agaataaatg atgaaaaagc tataaaatgg
    13621 ttagctactg gtgctcagcc aacagaagta gttaaaaaat tacttgtaaa aaacggagta
    13681 atagaaaaat tcgaagcttc taaacaagca aaataatttt ttcttggcaa ttagtttaag
    13741 taagggctca ttgctaaggt tactttagga gatgaaatat atgaaagagc tagttgtaga
    13801 tatagctaag gctctagttg ataatcctga ctcagttgtt gttgaggaat ttgaagataa
    13861 tgatggtatc gtcttaaagc ttacagttgc tcaagaggat atgggtaagg ttattgggaa
    13921 gcagggaaga atagctaaag ctataagaac tgtcgtaaga tctgttgcaa atagagaaaa
    13981 tataaaagtt tctcttgaga tagtataaaa taggaggatt aggtataatg cctagtcctt
    14041 tttatataaa tgcgtagttt aagattttat gatgatatga ccacataaga ttaaattttt
    14101 taagaaggtg ataacatgaa agaaaaatta actcatttta aggtaggtca aatagtaaat
    14161 acacaaggtt taaaaggtga agtaagagtt tatccgctta ctgatgatat agagaggttt
    14221 gatgaattaa aagatttcta tctgggaaaa gatttaaata ataaattggc tgttgaaaaa
    14281 gtaagataca aaggttctat ggttataatg aagataaaag gcatagacag catagagaaa
    14341 gcagaaaaac taaaaaataa atttatgtat gtagctagag aagatagcag agagcttgaa
    14401 gaaggcgaat tttttatagc agatatgatt ggaatggaag ttttaactgt agatggaaaa
    14461 tatgtaggga tactagaaga tgttcttcaa tactcagcaa atgatgtcta tgtagtaaaa
    14521 ggtgaggaag ataaagaatt tatgatacca gctataaaaa aatttgtacc tacaatagat
    14581 attgatgaaa gaaaaatgat tatagaccct attaagggaa tgatagatta ggtggtaata
    14641 tgagatttca tataatgaca ctttttccag aaatatttaa ttcgtacatg gatgaaagta
    14701 taatgaagag agcagttgaa aaaggtataa ttgaggttca tatatataat ataagagatt
    14761 tttctaataa taaacataaa aaagtagatg attatccatt tggaggagga gcggggatgg
    14821 taatgactcc tcaacctata tatgacacat ataagcatat aataacaact cataatataa
    14881 ataacccaag tgtaatttat cttactccaa aaggtaaggt atacaatcaa tctatggcaa
    14941 agcagatgtc tttaaaagaa gatataatac tactatgtgg tcattatgaa ggaatagatg
    15001 agagaatcat tgatttaata gttactgatg aaatttcaat tggagattat gtattaactg
    15061 ggggagagct cccagcactt ataatgatag attctatatc gagattaata ccaggtgtat
    15121 taaaccaaga agaatcattt gaagaagaat catttaaaga taacttattg gaatatcctc
    15181 attacactag acctagagat tttgaaggat taaaagtacc agaagtgctt ttatcaggga
    15241 atcataagaa gatagatgag tggagaagag aagaatctat aaggataacc aaagaaagac
    15301 gatttgattt atataaaaaa tctaatgaaa aataaaaatc taatgaaaaa ttaatatata
    15361 tgtatacaat aaagtaccta taaatggaat taaaaacata aaagtgcttg taataactat
    15421 tgaagtatag tataatataa aatgttgaaa atacgggcgt tcctctgttt acactggtcg
    15481 ggtagttaag aacgtctatg atatctagga ggaagaaaga tgaatgaaat gctaagagca
    15541 attgagcaag aacaattaaa aaatgaagtt cctaattttg gacctgggga cacagttaaa
    15601 gtacacgtta gaattataga aggaaaaaga gaaagaatac aagtattcga aggtgtagta
    15661 ttaaagagac aaggtggagg agcaagagaa actttcacag ttagaaaaat gtctttcaat
    15721 gttggtgtag aaagaacttt cccagttcac tctccaaaaa tagaaaaaat agaagttact
    15781 agaaaaggta aagtaagaag agctaaacta aactacttaa gaggtagagt aggtaaagct
    15841 gctaagataa aagaagctag aaataaataa ttaaaaactt tacttataaa tggggggctg
    15901 atttatcagt cctctttcta ttttaatgaa aattacagca aataattata ctagaaataa
    15961 atagaaatat ttgttgaaaa tatacattat ttatctatga atttatagct tttaaaatga
    16021 aaaataaaat tggtaaatat tgcattatat tttgtaacaa tattaaaaat aagagaacag
    16081 tattatgtaa agtatgacgt taaaaagatg acaatattat agcttgttaa gatagatttt
    16141 ttaagtggaa ttaggatata ataatataat attaataagg agtgatacat gtgtcaaatg
    16201 aatacgaaga gtatcttatg aatgacaatc tacatataaa ctggtatcca ggtcatatga
    16261 aaaaaactaa ggagttagta aaaaataact taaaattgat tgatgtagta attgaacttt
    16321 tggatgctag aatcccgttt agtagtaaaa atccagacat agacaggctt gtaggagata
    16381 agccaagagt tgtcgtactt aataagagtg atatggcaga tagggataaa ttgaatcaat
    16441 ggattgaata ctataagaaa attaatatca aagctatacc agtagataca ataaaaggcg
    16501 ttggtataaa taagataatc gaagaatgta aaaatgtaac tagagaaaaa atgagttctt
    16561 taaaagacaa agggagaaaa gaaagagcta tacgaataat gatagttgga gttccaaacg
    16621 ttggtaaatc gtctcttata aacaaattaa caggtagaaa aagcactcaa actggagata
    16681 agccaggagt aacgaaaggg aaacaatggg ttagattaaa gggaaatctt gaacttttag
    16741 atacacctgg aatactttgg cctaagtttg aagaccaaga agttgcgtta aatttagcat
    16801 ttagtagagc tataaaggat gagattttag atactgaaac tttggcttta agacttatag
    16861 agaaacttat gaaaatagaa cctgagaagt tgaaagccag atataagtta gattgtttag
    16921 gagaaactcc aattgaaact atggatatga ttggccataa aagagggttt ataacaggta
    16981 aaaaagaact agattataca cgcatagcca caactgtatt aaatgaattt agagatggaa
    17041 aaataggaaa tataacattg gaagttccag aaaacgttaa aagatagatt ttaaaacaaa
    17101 atcaaaaatt taatgttaaa aatttaaaaa agccttttag caaaggcttt tttatttttt
    17161 tgaaatatag acaaatatct ccatataaaa gtataaatta ggttagaaaa cctttaaaaa
    17221 tattaattta agacaaatat acctaatatt ttgaaaatta aaatgagtgt gattatttaa
    17281 aattttagca tattgacttg cttgtttcgt ataatat
//
LOCUS       DAEORZ010000087        17090 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEORZ010000087 DAEORZ010000000
VERSION     DAEORZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.087"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(176..700)
                     /locus_tag="KRH39_002482"
     CDS             complement(176..700)
                     /locus_tag="KRH39_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG8512380.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(765..1517)
                     /locus_tag="KRH39_002483"
     CDS             complement(765..1517)
                     /locus_tag="KRH39_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG8512381.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1548..2846)
                     /locus_tag="KRH39_002484"
     CDS             complement(1548..2846)
                     /locus_tag="KRH39_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBG8512382.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRAVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2863..5127)
                     /locus_tag="KRH39_002485"
     CDS             complement(2863..5127)
                     /locus_tag="KRH39_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBG8512383.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5382..6200)
                     /locus_tag="KRH39_002486"
     CDS             complement(5382..6200)
                     /locus_tag="KRH39_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG8512384.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6193..6891)
                     /gene="cbiQ"
                     /locus_tag="KRH39_002487"
     CDS             complement(6193..6891)
                     /gene="cbiQ"
                     /locus_tag="KRH39_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBG8512385.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6894..7184)
                     /locus_tag="KRH39_002488"
     CDS             complement(6894..7184)
                     /locus_tag="KRH39_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG8512386.1"
                     /translation="MSAKTKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAES
                     EITKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7174..7926)
                     /locus_tag="KRH39_002489"
     CDS             complement(7174..7926)
                     /locus_tag="KRH39_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBG8512387.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8108..8300)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8432..10204)
                     /locus_tag="KRH39_002490"
     CDS             complement(8432..10204)
                     /locus_tag="KRH39_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBG8512388.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10218..11147)
                     /locus_tag="KRH39_002491"
     CDS             complement(10218..11147)
                     /locus_tag="KRH39_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8512389.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11375..11563
                     /locus_tag="KRH39_002492"
     CDS             11375..11563
                     /locus_tag="KRH39_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8512390.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11556..12257
                     /locus_tag="KRH39_002493"
     CDS             11556..12257
                     /locus_tag="KRH39_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8512391.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12259..13182
                     /locus_tag="KRH39_002494"
     CDS             12259..13182
                     /locus_tag="KRH39_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8512392.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMETEFTILLPKAL"
     gene            13339..14262
                     /locus_tag="KRH39_002495"
     CDS             13339..14262
                     /locus_tag="KRH39_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512393.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVIVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14255..14992
                     /locus_tag="KRH39_002496"
     CDS             14255..14992
                     /locus_tag="KRH39_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512394.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANITLGRRK"
     gene            15003..15776
                     /locus_tag="KRH39_002497"
     CDS             15003..15776
                     /locus_tag="KRH39_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512395.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16317..16925)
                     /locus_tag="KRH39_002498"
     CDS             complement(16317..16925)
                     /locus_tag="KRH39_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBG8512396.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5509 a         2964 c         2150 g         6467 t
ORIGIN      
        1 taaaacttta ttttttagtt aaaatcatac aatatataga tttccacaat acaaataaat
       61 taaaaagtga aatgtatttg tctaaaaaca taataacatt tcactttatc ctcattttta
      121 agtaatatat atttttaatt tttatttata actaaatcta gtttttatat aaattttagt
      181 ttttttctac atctgagtaa tctattccaa aagtttctac agtaacagat ttcataactt
      241 gtggagtttg tggcttatca gctgaatctg ttctaactga tgctatttca tcaactgtat
      301 ccataccttc tatcactcta ccaaaacctg catactgacc atctaagtgt ggtgagtttt
      361 tatgcattat gaaaaattga gaaccagctg aattaggtgc catagttcta gccattgata
      421 tgactcctct ttcatgtttt aaattgttag taaatccatt accactaaat tcacctttta
      481 ttgagtgacc tggaccaccc attcctgtac catttgggca tcctccttgt atcataaatc
      541 ctggtattac tctatgaaat attattccat tgtaatatcc ttcatttact aatgatacaa
      601 agtttttaac tgtatttgga gctatatttg gatataactc aattttgatt tcttttccat
      661 tttccatttc tatagttact ataggatttt tattttccat aatttattac ctcccttttc
      721 attagttaaa ttacataatt acatttatta tattatactc taaattaaag tctatcaagt
      781 atattttttc ctatttcata tccttcttga taaatagttt tacaaaactc atcatcaaag
      841 ttaagcgtgt ctttaggagc aaattgagta ttaggtctaa ctactattat tttatcttca
      901 tcatatactt ttttaatatc ttctggcaac tcataattat attttgtgga tattaaaata
      961 attttatcca cattatcccc aattaatggt tcaattaatg cactcctaat taaacctcca
     1021 tctaatttga aaccatcata aattccttct tgcacatctt tcacaaattg ttctttaaat
     1081 ccaagagttg cgtttggatt tgatggtaac aacccactgt atgaaatgct gataatggct
     1141 tcattacgag gcatttttga tacatcaaca atcttttctg atactacttt attatcaact
     1201 tcaacataat taacataaaa gttcatttct tctatattac tatcatctag ttcccttagg
     1261 ttgtctatag caggagaatt atccacagta ttatttacta tttttacatt tccattctgg
     1321 atatctttta tatttgtcca cattttctcc aagttatcaa cattccctgt gtacaaataa
     1381 tatccattta ttgctcctat tgatgtccct gaaatcgaat aaaatttatt tattcctcca
     1441 tcatacaagg ctttaactac tcctgcttga taagcccctt ttgctcctcc accttctaaa
     1501 cataatccta atttcatagt ctcatctcca aattgtttat cttatattta tatttcaaaa
     1561 aacttactaa atgttttata tatactatag tggtcctcaa catttcctaa ttctcttata
     1621 gagtgcattg ctaaaattgg acttcccaca tccacagatg gtatatctat atgagtagaa
     1681 gatattggac ctatagtact tcctcctctt tcatctgacc tattaacaaa ttcttgatac
     1741 tcaacattag cttctctaca tatattttta taaatagcta tagaatatcc atcactagta
     1801 tatgcctgac tagcatttat ctttataacc ggaccttttc ccattattgg tttattagtt
     1861 gggtcatgct tttctggaat gtttggatga atagcatgag ctaaatctga tgatatcata
     1921 aaagatgaat aaatagactc aaagaatccc tctctatcct tacccattga tatacaaatt
     1981 ctctccaata tatttaacaa catgtttgaa tctgcacctt gtttagtaga gctaccaact
     2041 tcttcattgt caaatacagc cattacattt acaccatgtt gacctttagt attaacaaga
     2101 gcattcaaac tagcatgtac cattgcaaga ttatcttgtc tcccaataga tataaattca
     2161 tcattagccc ctattaaact tcctttttca aattcatata aaaataaatc aaaatctata
     2221 atatcgtcca tatttacatc taagttagat gctatttgct ttataagaaa attatctttt
     2281 tctaatgtat catttaacat cccaactaaa ggtaacatat ctttttgctt attatatgaa
     2341 tgaccatcat ttatacttct atttaaatgt attgctagat ttggtattat acaaattggc
     2401 tttttaaagt ctataagcct ttcatttggt tttaatatac tatcaccttt taaaactgct
     2461 ctaccagcta tcccaagagg tctatccaac catgtactta atattgctcc tccatagcat
     2521 tctgtattta acttcaaata agatttttca gcttctattt ctgcattcgg tttaatacta
     2581 aatgtaggag aatctgaatg agagcctatt attctaaatc catctctttc tatatcttct
     2641 gaatttacta caaaagctac tatagctgat aaattcttag taacataata ctttccacca
     2701 actcttagat tccatctttg attcaaaact aattcttcaa atccattttt atctaagatt
     2761 tccttagtac tctcgactgc atgaaaagac gacggactat catatatgaa gtctattaag
     2821 tttttagcaa agtttatact atccattcct catttacctc cattataaaa tatttagtgt
     2881 tgtatattct ttatttattt ttttaattcc ctgttggtca aaagctatag taacatctgt
     2941 accaattata gaaaccactg tcccaacccc aaatttagga tgatgtacct tagaaccaag
     3001 ttttatatca tctggattag tttctttatt agaatctttt attgtagcat taactttatc
     3061 tgcaacttta gatttgctta tagtactcat atacttttta gtatatttat ctaacatatt
     3121 ataacttgct ttagaatagc ttagttcttt ttttactttg tacaatcttt ctatacattc
     3181 ttctggcagt tcttccataa atcttgaagc tattgcaaca tttgttttac catataatgt
     3241 tctcttctct gtcaatgaca tatatagctc ttctttagct ctagttatac caacataaca
     3301 aagtcttctc tcttcttcta tttgtgaatc actcatagat tttatagctc ttgatatagg
     3361 aaataaccct tcttccatac ctgttaaaaa cacaacaggg aactctaaac ctttggatgt
     3421 atgaattgtc ataagcgaaa ccctatcatt gtcttcttca tcatttgatt cagatgtaag
     3481 tgctatactt gttaggaatg tctctaagtc tttattttca taagtatcat cattactact
     3541 ttcaaactcc aaagctatag aaataaattc tctcaaatta tcaattctgt cttgagcttc
     3601 ttcacctttg cctgttaaat ctttttcatt tttatttctt atctctacaa gctcgtccat
     3661 atatcctgta gtatccaaga ctttttctat aagtttgcta acaggataaa cttcttttat
     3721 agttctaaga gtccctataa tatcaacaaa tccacttata cttgcccttg ccttagtaga
     3781 tatatctgaa tttgtttcta tatcaattaa tactgagtat atactttcct gttttaaatt
     3841 agctctatct tcaatttttt ctatagttct aagccctata cctcttctag gaacatttat
     3901 aatccttttt atagatatat catcttgtgg attttgaata actcttaaat acgccaacaa
     3961 atcctttatt tcttttctct catagaattt tgtaccacca tatatattgt atggaatttg
     4021 acttctattt aaagcgtcct ctacaggtct tgcctgtgca tttgctctat aaagtactgc
     4081 aaaatcctta taaggtctat tttcttttct agctatttgt gcaattgaat cagcaatgaa
     4141 atcagcctct tctatttcac taccagttaa ttgtatcttt ataagttctc cttctttttt
     4201 ttcactccaa agcttttttc tttttctttc tatattatta gatataactt tattagctgc
     4261 atctaatata acttgtgtag acctataatt ttgttccaac tttacaacat gtacattgtc
     4321 ataatctttc tcaaattcaa gtatgtttct tatatctgca cctctccatc cataaatact
     4381 ttggtcatca tctccaacaa cacaaatatt ttgatgctct cttgctaata attttatcaa
     4441 ttcatattga gctttacttg tatcttggta ctcatctacc attatgtatc taaatctact
     4501 tctgtaatat gctaacactt catcattagc cttgaaaagt tctacagttt taagtattaa
     4561 atcatcaaag tcaagtgcac tatttctttt taaacggtct tgatatagcg cataaatatc
     4621 tgcaatctta accattctat tatctgccat attgatatct ttaaactgct ttggagtata
     4681 aagtttgtct tttgctcctg aaattgcact tataactgct tttggttcaa acactttatc
     4741 acttaaattt aattctttta gacagtcttt aaccaatgta acttggtctg ctgaatcata
     4801 aattacaaaa cttctattgt aaccaatctt atttatatct tttcttagta tacgaacaca
     4861 gcaagaatgg aatgtactta tccacatatc cttagtttca ggacctatat tttgttcaac
     4921 tctctctctc atttcatttg cagctttgtt tgtaaatgtt atagccaata tatttggtgc
     4981 ttgtacacct ttatcttcta taagatatgc tattcttgtt gttaatacct tagttttacc
     5041 tgaaccagca ccagccagta taagtactgg accttcagtc ttttctactg cttctctttg
     5101 agcaggattt aatgtatcta aattcatatt tctcctcctg ttaacagcca taaatatatt
     5161 ttaatctaaa atatgaatta cttcaaatta taattatgta ttatattaat agattatttc
     5221 caatatatta caattaaata acatttttcc atataattta tttcattgtt taataaaaaa
     5281 aataacctta tgatttgggg aatcataagg cacctttaag gggtctaggt taagggttat
     5341 tatatataaa tctatttttt taaaataggt ttagggctta attatttatt tgtattaagt
     5401 tctctttcct tccaatactt atacaaatct tcttctttct taaataaggg taaattcata
     5461 tttctatgta cttttactaa ccatggggat tctagaccag cttgtataat attattttca
     5521 tttataaaca cattttctgc ctttccttca tcaatcaaaa tccccttatc caatacatat
     5581 atgtaatcac atatttcata cattaaattc atatcatgac tggatattac tatttttata
     5641 ttatttttat ttaatccctt tattatatct acaatagacc tagtacttac tgggtctaaa
     5701 ccagctgtag gttcatcaag taatacaatt tcattttcca ttgctataac tgatgctatt
     5761 gccactcttt ttttctgtcc atagctaaga aaatgtactg gcctgtctat aaaatctatt
     5821 ccattaacag ctactagagc tttgtttatt ctctctttaa ttgttttttc atccatacca
     5881 atgtttctca ttgcaaaggc tatatcatca tatacttttg aataaaaaat ttgtttttct
     5941 gggtcttgga acacaatacc tacatttttt ctaaggttgt ataaacttct cttatcatat
     6001 tttaactttt cttctttaaa aagaatttct ccagatgtag gctttaatat acccattaaa
     6061 ttcataaata atgttgattt tcctgaacca tttgaaccta ttatacctat aacatttccc
     6121 tttgaaaaat ccatattaat gtttaatagt gcattggtat ttttttcata ttgatacgtt
     6181 aaattattta ttttaaacat cattatcccc tactatgtga aatttaccat catacaattt
     6241 tatgtctaat gatatacaca tatcatcata ccttgtcata agtcttttgt acagcatact
     6301 tccaagaata cctaaggatt tatatgaagt ttttaaattt atatatccaa atcttagctc
     6361 ttgtgacttg tatatttctg aaaattcttc taaaaatatg aatataaacc tatatattaa
     6421 cattgataac tcgacaaaag tgtcaggtaa atgaagtttt ttaaaaaaga atattaattg
     6481 attaaaaggt gttgtaagta tacaaaaata cacacaagtc aaacaagaca tcgctctaaa
     6541 taacacatga atagacatgt caatagacgc ctttgatatt cctacattaa aactaaaaaa
     6601 ttcaaaacta tataataaat ctgctttatt aaaagatata tttacaagag ttattcctat
     6661 actaagaaat aaaaagtaca ttggtattct aagtaatttt aaataatttt ttaaatctat
     6721 tccagccaca caaacaacca aaatactcat taaaatcata attccactta aaataaatat
     6781 attttgtatt accatagaag caatcagaaa aattactcct atggctactt ttttgtttgg
     6841 atttacttta ctcagtctat ttgtatatgc atatttatca attataagca tgattaactt
     6901 ctctttcctt tttgaagacc taatatgtat cctattacac cagcccctaa agctgcttgt
     6961 gcagagaata ataaactttc tatttctcca cttggtggct catatggact gctaaaccat
     7021 ggtttgtaat ttggatttat cttagtaatt tcactttcag cttgaccatc tgctcctcca
     7081 tattcagcac ctgagttgac aagtaatggg aatataataa gagctactac taataaaagt
     7141 aataatatgt tagtttttgt ttttgttttt gttttagcac tcattagctt taactccttt
     7201 atcattattt tctagtatgc ttttttctcc gttttctgta attaaattat atacaattac
     7261 agtaagtata ccttctgcta ttgctattgg tatttgagtc ataaagaaaa ttcctaaaaa
     7321 cttaattgct gaagccatca ctccacctga tgggtctggg aaagccaatg ctaattgtat
     7381 tgaggttatt gtatatgtag ccaaatctcc tattgctgct gcaagaaata ctggcattgc
     7441 attatttccg tcttttttct ttaaagcctt aaatattagg aatgatacga ttggccctat
     7501 tatcgccatt gaaaaagcat tagctcctaa agtagttatt cctccatgtg ctaacaacaa
     7561 tgcttggaat attaaaacta tagttccaag aacaaacatt acgcttggtc caaacattat
     7621 tgctccaaga cctacaccag taggatgtga acaacttcct gttactgaag gtatttttag
     7681 tgctgataat acaaacacaa atgctccaca taaagctaat aatactttct ttttagggtc
     7741 ttgctttact attttaccta ttgattttaa tccgaataag aaaaatggta taaatataac
     7801 tccccatgct atagaccatt ttactggtaa gtaaccttcc attatatgca ttgcatttga
     7861 tgtaactgga gttaaaatga ttaacataag tgcagctata acccctaatt taatattctg
     7921 tttcatctta gtatccctct ctccctttct aataattaaa acataataat acatgtctga
     7981 tttatttaat aaaataaaaa agcctaactc gatggttagg cttctaattt tccacaatct
     8041 aaatacaaac acataaaaca aacatatctg tataattatt ataagaaact ttctccatcc
     8101 ccgaagaaat tactttaata ttttaggcag gtctcctgac ttagcttcat cctactcctc
     8161 ttccttccca tgagatataa aaaatatcat catagtggct aacaaaagtt tcgtctgcat
     8221 cacagtagcg ggggctgtaa aggaatttaa cctttttccc tattaatctt gcttaaagaa
     8281 cctataatat tatatattta atttttaaat tattcttaaa ttattttcta tataaatcat
     8341 tgtatatctt ttctgtgaaa atttctacat tttttataaa ttttattcat ttttatattt
     8401 ttatgtatat aaattctttt ttgtaagttt attaatttct aattatcttc tttaaataca
     8461 ttgaaactac atcagaaaat ctagttatat ctcttgaata tataaaatca tttccatata
     8521 ttaatctttc agcttcaagt tctgattctt ttcctgtaaa tactcctagt actaatatat
     8581 tttgctttct agcttttctc acttcattgg ctgtatctct caaaccaact atacccctat
     8641 aagccttttc tcctcttaat gttctctcac tactttttcc tatctttaca tcatttggtc
     8701 ttccatcgct gagaactatc aggattttat tttcttcatc tctatttatt agaccactac
     8761 atatagattt tattgccaaa ccatctctat tattaccagc acaaaaatat tcaaatatat
     8821 tttcactatt ttttatacta tcatcataat ctttaaatct ttttaatatt gtatagtcta
     8881 aaaaactaga aaatcccatc actctatttg gtatgccaac ttctgttaga gcatttgcaa
     8941 ttatgtaagc ttgaattgcc acatttccct gatttctact ttgagagcca cttgcatcca
     9001 ataatatatc aataacgtat ttcccttttt cattttctat atctttataa aatattttat
     9061 tattattact tctaccaact ctccaagctc tatttgctaa tatagtacca caatcagaat
     9121 atactctact tttagcattt tcttctatta aaattcttga cagactttct tttaatctct
     9181 ttatttgctg tctatacatt ctagcatgtt ctctaaattt tcctaaatta ttttcttttt
     9241 gtcttgttac atattttagt tgaaatacat tattacactc acttctaaga actccatctg
     9301 taaagtgtat tctacaacct tcatgaacat ttctacaatt ttgtgtctca atttttttaa
     9361 tttcaccttt actcaaataa gtttttccat aataatgttc tatttttgaa tatatcttat
     9421 ttgcagtgtc ttcatcaaca tatataactc ggtctgctga attgctacat ttagaatcat
     9481 caaaatcccc tacaccttct acaagcatgg atgaagatat tgaatctata tcactttcaa
     9541 taatattttc ttcttcatca ctataaagtt cttcatacat gaaatcagca aatgtatcaa
     9601 aatttacttc tacactttct acattatttt catcaataag actattttct tctccattta
     9661 atatgtattc aaaataagtt aaatatataa catctaatgc ttttaaaatc tccataatat
     9721 cttgattggt atctatattt cttatatcat taactattct tctagtcata ctatctaatg
     9781 ctgggtgctt gtccatacta taaagtacaa atgtgtattt aattttatcc ataatattat
     9841 cattgtgtat tttggtaaaa cgtgaaacaa tatctttata agcatttctt cttatgtctt
     9901 ctatccctgg cctgtcttta atgacctttt cttcaactat tgtatttaat ataacttgaa
     9961 ttagacctag tataacatct ttgttgtatc cttttcttat tctatttgta agatattttt
    10021 taactatatc ccaatctaca tactttcttc tagcacctgc aattattgcg aaatatagac
    10081 ctatttcttt tgaagagtaa tctttttctg atatatctat actttcattg taatcaccag
    10141 ctattgtcca tgataggtta ttagctctat tttcaaattc ataatcatca tatatccatt
    10201 tcataatctc acctatatta aatatgactt attggaaaca catctatact ctcccattta
    10261 ttaggtattc tagttttaat tacatctcca accatttctt tctcatatac atcaaaagtc
    10321 tttcctgtaa gtcccatatt aattgcaaga gttggtttta atcctcttct tatagtctta
    10381 agagatgcca taagaccacg taaatcaata gctttactag atatctctcc attttgagac
    10441 ttcaattgta aatctaaaaa tattcctgca aagtgtatta atttttcttc atcagcatct
    10501 gaaaattcat tctttaaaat catcattaac ttatcttctt ctataggagg aatatctatt
    10561 accataaatc tagataccag tgcttcatta agttcttttg ttcctgcata ttcataattc
    10621 atagtcccaa taaatcttgt tgctggatgt agattgactc tctcataccc aggtacatca
    10681 attattcttc tatagtctaa agctgaatgt accacaacta tagcatcatt ttttgccata
    10741 ttaatttcat caaatacccc aaaacctcca ttaatagcac actcataaac tgagcctctt
    10801 ctaagtttaa cttcattgtc tataaatgta tctgtaccta taagtgttga actgtctgta
    10861 tttatatgaa atgatgtatt ccactgaggt ctattaaata tttcccctag attatcagca
    10921 agtaagtttt ttcctgttgc ctttggtccc gataataata tattttcttc ttctaaaata
    10981 gctgctatac acatagacaa gatatcttta ccataaaaat aagttttaga ctttgttact
    11041 ctgtattcta aatctttttc taatccataa taatttctaa aatacagtac atcttcaata
    11101 agtttttcat ctaccccttg cacccttaga ttatctatta tatccatatt tttacccctt
    11161 ttatataaat agtatttgtt tcatttctta tcaccatttt agcataaaag gatataagct
    11221 aaaattgatt ttttaatctc cccattttaa gtaaaaccat gcaccatttt ataactacaa
    11281 aattaagagt actacttact gatatttctt gattttattc gtctatacgt atataatata
    11341 tgctgataaa ttatgaggaa aggatgattg aaatatggaa tatatgtctt gcccagaagt
    11401 agcaaaaaaa tggggtattt ctgaaagaag agtacaaaaa ctatgtgaag aaagtcgcat
    11461 accaggagtt tcaaaactcg ggtatatgtg gctgatacca aagaacgcag aaaagcctgt
    11521 tgatggtaga acaaaaagaa gaaaggaagt acaatatgaa tagcgttttg attatagatg
    11581 atgacaaaga actttgtgct cttatgaaaa aatgtgttga gcaagaaaat ttatctgcta
    11641 ttactgcaca ttgtggtgca gaaggtctac atttggtcga taaaaataga cacacactct
    11701 ctcttgtaat tttggacata atgatgccag atatagacgg ttttcaagtt ctgaaaaaaa
    11761 ttagagaaac aagtaatatt ccagtaatta tgcttactgc taaaaatgac gaggatgata
    11821 aagtttccgg actacgttta ggtgctgacg attacctaac aaaaccgttc agcataaacg
    11881 aacttatggc tcgtgtcaat tctcttattc gtcggtatac tatgttaaac cctgctttta
    11941 taacagacac agattgcata ataataaatg gcatgattat tgacaagaca aaccgaattg
    12001 tttctgtcaa taatattcct gttcaactta caagcaaaga atttgattta cttgcttttc
    12061 tggcttctaa taagggacgg gtgtttacca aaaaacagat ttatacacaa gtatgggaag
    12121 aagaatatgc ttttgacgat agtaacatca tgtctttcat cagtaaattg agaaaaaaga
    12181 ttgaaccaga ccccaaccat ccattttata ttttgacagt tcgtggtgtc ggttatcgtt
    12241 ttaataagga ggcttaacat gaatacaagt gtctatctgc ttttgatatt ggttcttagc
    12301 ctgattatta tagcgtacct tgtttctgtc ctgctacgtg tccgaataca acttacactt
    12361 ataaaagatg ctttagagga tataaaaaat ggaaacctta atcgacgtat tttaacagat
    12421 gaaaacgaca ttacaaaaca aatttgctat gatattaacg aaatagcaat caatagccaa
    12481 tttcaactta ttcagcaaaa acaatcagaa caagcctaca aacagctaat gacaagtctc
    12541 tcccatgatg taaaaactcc acttgcttct ttggtcggtt atttggaagc agtagaaagt
    12601 aaaattgttg taggagagga aaaagacgag tatatccatg ttgcgtctaa taaggctcat
    12661 tacttaaaga attttgtaga aaatctattt gaatgggtaa aattggattc taaggaacaa
    12721 gtttttcatt ttgatatttt tgacttgaat gaattatccc gtaatattat atctgattgg
    12781 atacctgttt tagaaagcag tcattttgaa tatgaatttg atataccaga aatagagtac
    12841 ttcttacgta tagacgcaaa tgcatatacc cgtatcatta ataacctatt acaaaatatt
    12901 attacccata gtagtggcaa caaaatgaca ttgagaattt ttgaaaacaa agagcaagct
    12961 caaattataa taacagataa tgggaaaggt atatcttcag acaatctccc acatattttt
    13021 gaaaggttgt accaatgcga ccattcacga gcttcaaagg gaaatggttt aggcttggcg
    13081 attgcaaagg aacttattaa cgctcataag ggaaatatta ccgccaacag cactcccggt
    13141 atggaaacag aatttacaat attgcttcct aaagccctgt aaaagtacga ggctttctta
    13201 ctctaaaaca agataaaagc aagattatgg caaggttttg gcaaggttca tgttttataa
    13261 ttctaattgt agttgatttt gacgttgtat caactatctt ttgtagaaac aaatttatta
    13321 aaagtgagga aattatatat gaatgatttt gtgattgaaa caaagcaact tacaaaaatt
    13381 tatgggaatc aaacagtagt tgacaaagtc aatctccatg taaaaaaagg ccgaatatat
    13441 ggtctgttag gacgcaacgg agctgggaaa accacaatta tgaaaatgat tttaggattg
    13501 acttcaatca cgtatggcga ggtagatgta tttggagaga atattaaggg acgtgaaaaa
    13561 caagtatatc cccgtatcgg tgcaattatc gaaacgccag gattttatcc taatctgaca
    13621 ggaacggaga acttggagat ttttgcaaaa ctgcgtggta cagccgcccc taattctgta
    13681 aaaagagcat tggaaattgt aggcttacct tataaaaata aaaagctgtt tagtaaatat
    13741 tcacttggca tgaaacagcg acttggtatt gccaacgcta tcctgcatga cccagaactg
    13801 ttagttttag atgagccaac caatggtctt gaccctattg gtattgcgga gatgagaaag
    13861 tttattaggg acttgagcgt tgagcatgga aagacaattc ttatttccag tcatattctt
    13921 tcagaaatct cattgttggc tgatgatatt ggaattatcg accacggtgt tcttttggaa
    13981 gaaagtagta tgagtgaact agaaaagaaa aacagtaaat atatcctgct acaagtttca
    14041 gatattccga agacatcact tattttagag cgtcaattta acacgaagga ttattccgtt
    14101 caagatgact acacgttaca cctttataac actaatctgg atatgggtga aatcaataaa
    14161 gcacttgtaa tgcagaatgt aattgttatt agttctcaac tttgtaatga tactttagag
    14221 gactacttta aaaaaatcac aggaggagag ggcattgctt aaacttattc aaacagaatt
    14281 tcttaaactg cgtcgtagga aacttatttg gctgatgttg ttgtctgctc tcattatgcc
    14341 gtttttcgca ctactatact tcaattattt tggtaaaaca ggtgttgagc caacaaagtt
    14401 ttataaatgg tctgcatttg gttatacact ttggattatt ttgcccgttg ttttgggtat
    14461 attttgcacc atgcttatac acgaagaaaa ccagtatgat atgctcaaac agctttggat
    14521 tgtacctatt agtaaaatgg gatacttttt tagtaaattt ttcgttgttt tgatttactc
    14581 tatttgtttt atgctgatta cagccgttgc ttctgtgtta tttagtgtac tttctggata
    14641 tgtcgtattt agctgggcaa gcattttata tttgttgaaa aaatgtttag aaattggtgt
    14701 aatcactgct tttgtaatgt tgccgatttt ggcaatcgct acatctcaaa aagggtacat
    14761 tctcccaatt tgtatcacat tggtatatgc cttttccggt tttgttataa tgacaataaa
    14821 tatgtattta cacccattat ccagtatggg agttattatc atgagaaata aagacattcc
    14881 aggtcttata ttttcacagg aaattaatgt cccattagca tttctttgta tttgtatttg
    14941 gagtattgta tcagtattgt ttgcaaacat tacactagga agaagaaagt gaggtgtaga
    15001 taatgtggaa attactttgg gctgaatgtc aaaaaattcg tcgttcaaaa attgtttgga
    15061 ttacagtttt tgcatccatt atgatagcag tcatagtttt tgcagggggg caggatgtat
    15121 ataatgaccc agatttacat tatggcttaa agagcgttta tgacggttca cggtatatag
    15181 acaatgcagg ttggtatatg gacgcagtac aaccttggtc aacttttttt gttcttcctg
    15241 ctgtcattgc cctattagga agctatatta tttgtcgtga agaagaggag gacactatta
    15301 aatccctgcg tcttattccc ataaatgaaa caaatttaac catagcaaaa atgattatta
    15361 cttttatatt cagtatactt ctttatttgt tactctttac tataacattt ttgactgaat
    15421 ccgttttaca tttttccgat ttatcgacaa aactggtttt aagttgcatg aaagagtatt
    15481 ttcttgatgg cataggtgta ttctttgcaa tttctcccat tattgccttg gtatcgcgca
    15541 tgaaaaaagg atattggctt gcattagtat tcacagaagt ttattctatt gctggactat
    15601 ttgcgggtat gtcaaatgtc ttacaaacct tttttcctat tgcggcaata tttaatattt
    15661 ctggatacta tataactact ggagaaaaaa ttatggggag tatcataagt ttattacttt
    15721 gtgcctgcct ttctgcattt atacttaaag ggctaaaaca caatgaaaaa aattgaaata
    15781 ggtatatcaa aatgaaaaag ataaaaaaac tgattattct aattgtattc ttgtttcttt
    15841 ttggcatgtt acactgttat actgaatgga aattcaacaa taacccacct aaaggggtaa
    15901 tagaaagaat aaatgagtgg actaataaga ttggactgga ttagtaactt atgttaatta
    15961 tagtgaatgt aatattagca agttattgtt tcgtatttta atactataac tttaaatatt
    16021 tggttttaat cttccaaacg aatataaaaa aactgtcaca gaggcaacat atttttgaat
    16081 atttataaag tggtagtttt gattttcaaa gctgccgcct tttcttctat ataagcaaac
    16141 ttatcctcaa caaagtgtaa aacatggtac attttaagaa aaaagtacca cagacacaag
    16201 caataaaaga aacgccgaga acctacaaaa acgtagtgct tatgcagatt tacgacgcct
    16261 tctattttct caaccgtctt aatgaccttg tacaaaagaa atttttctaa tattttttat
    16321 aatcttatct tgtgatgttt tttatgtata aatgacttaa taacaaatcc aataaaaatt
    16381 atggatattg gtataattag aacgaggaat ttataatcat tataatgtga cattattctt
    16441 acccaactct cagaaaaaat atatcctata ctgattaatg aagtattcca tatagttatt
    16501 cccacgatag atgatattac aaaagttata atattttgac ctgctatacc tgctataaaa
    16561 gatatgtatg ttctacataa agggattaat ctacatacac aaacagaaat agaagagtat
    16621 ttattaaaat aatcttgaga tgcaaatatt cctttctttg cctttggaca tatctctatt
    16681 attttattta tgatagattt acctccccaa tttcccactg tataacaaaa tatcgaacca
    16741 agtatacctg caattatact taaaattaaa attgtcagaa atccaaaatg atttcttgca
    16801 gcaattgctc cacataaagg taaaacaact tcgctaggta atggaaaaca agcatactca
    16861 atcataatta atacaaatac agagattaat ccatattgta aaataaaact attgattaca
    16921 gacacttact tatctatctc ctttcttatt taaaggtttt aagggtacat gttctaagga
    16981 ttctcaccct atatttaaag aatcaatctc tttaaactat tatatgtatt tacacaaagc
    17041 atttataaca tactttaaaa atagtacctt aaaataagaa acttaataaa
//
LOCUS       DAEORZ010000088        17068 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEORZ010000088 DAEORZ010000000
VERSION     DAEORZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.088"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..677)
                     /locus_tag="KRH39_002499"
     CDS             complement(<1..677)
                     /locus_tag="KRH39_002499"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG8512397.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKLLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYG"
     gene            complement(1300..1839)
                     /locus_tag="KRH39_002500"
     CDS             complement(1300..1839)
                     /locus_tag="KRH39_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HBG8512398.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            complement(1916..3721)
                     /locus_tag="KRH39_002501"
     CDS             complement(1916..3721)
                     /locus_tag="KRH39_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8512399.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLSKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(4117..4494)
                     /locus_tag="KRH39_002502"
     CDS             complement(4117..4494)
                     /locus_tag="KRH39_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG8512400.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            5399..5884
                     /locus_tag="KRH39_002503"
     CDS             5399..5884
                     /locus_tag="KRH39_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512401.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(6084..6623)
                     /locus_tag="KRH39_002504"
     CDS             complement(6084..6623)
                     /locus_tag="KRH39_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8512402.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(6616..7029)
                     /locus_tag="KRH39_002505"
     CDS             complement(6616..7029)
                     /locus_tag="KRH39_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8512403.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(7065..8186)
                     /locus_tag="KRH39_002506"
     CDS             complement(7065..8186)
                     /locus_tag="KRH39_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG8512404.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            8785..10437
                     /locus_tag="KRH39_002507"
     CDS             8785..10437
                     /locus_tag="KRH39_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBG8512405.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKIENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNIIYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            10736..11485
                     /locus_tag="KRH39_002508"
     CDS             10736..11485
                     /locus_tag="KRH39_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512406.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYVASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            11498..13525
                     /locus_tag="KRH39_002509"
     CDS             11498..13525
                     /locus_tag="KRH39_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512407.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFFYKTPLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQNSKKNKRTFYTDIK
                     IIAIPNFIYRIRSNSKALIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            13669..15252
                     /locus_tag="KRH39_002510"
     CDS             13669..15252
                     /locus_tag="KRH39_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8512408.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSAYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDKKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESIS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKDI
                     IKLNISDNGVANTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGAINISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            15224..15844
                     /locus_tag="KRH39_002511"
     CDS             15224..15844
                     /locus_tag="KRH39_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8512409.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLINSLLKVYQGVTCFPSENTEVIIEDLTTMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(16027..16953)
                     /locus_tag="KRH39_002512"
     CDS             complement(16027..16953)
                     /locus_tag="KRH39_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG8512410.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         5968 a         2622 c         2068 g         6410 t
ORIGIN      
        1 ccatattctt ttaattgttc tatagcttct ttagccatag ttccatatcc ttgcattatc
       61 catgctggta tttcttcata tccgtcccat gctgtatctt gaaccattac tccatggtca
      121 tcagcttctg cataatcatt agctaatctt actgcatcat catagtttaa gtctgttata
      181 ctagcatctg caccttctcc tttgatttta tttaatctaa attcagaaga accttttggc
      241 attaatacaa cagatttttg acctaattta ttagctgtcc aagcaactcc tctaccatga
      301 tttccatctg tagctgtaaa gaaagttatt tctcctaatt tttcttttat ttcttttgaa
      361 gttaatttat cataccctaa ctcagatata tctgtattta atctttgagc taagtatcta
      421 cccatagagt aagaaccacc taaaacttta aatgcgttta agccaaatct ataagattca
      481 tctttaacat atattccgct tacgcctagt aattttgcta agttatctaa tttaactaaa
      541 ggagtctttt catattgtgg aaagctttca tgaaaagctt tagcctttgc aatctcttca
      601 tcttttaaaa attcaattcc tttttctttt tcctctgatt taggtaagtt atttaatttc
      661 cattttattt ctctcattta ttttatattc ccctttcaaa tttttcggtg tcattttaat
      721 tttttatctt tgtgccttat agcaaagcaa ttaccgtgcc aatattattt attctaaaaa
      781 caattttttt atgttggaat tacatacttt tataaaattt ctttaaagtt tataaatttt
      841 gagtgtgaaa aataagattt ataaaagaaa aaattctcat attgggaaat ttttatcaat
      901 acgagaatta tcaattcgag aaatcgttta tttatcttaa ttcttccttt attatgctct
      961 tattttttat atgtctattg ttttgtcaga tatattacta cttttatttt ctataaatat
     1021 atggctataa ccttcttacg cataaactct ataaatttat atactttata ttctttatta
     1081 cccaaatatt ctttattacc caaagcctcc aaaatggctt aaatcttttc atagcttctt
     1141 acagaagttt acatgagcgt ccccccaata agcaaaagtg caatatgtgt accattgtgc
     1201 cctacttgcg actattgagc tgtcgaaatt cgcaacttcg catttatctg atgaaatgca
     1261 ctatgcaaaa tattcataac atccacaaca gagttaaagt tacctattca tattcactgt
     1321 aaaaatttct tccacaggaa atgtaatttt aaccaatcct attgagacaa actcaccact
     1381 tatttttttg aactcacgca gtaatacatc acttgcccga aaaaaagcct cactttcatc
     1441 atctccagta agtgctattg tgcaatgggg tacccaacga ttggggtggt accattccca
     1501 accatttgaa tcaaaatctt gtagtacctc atataattct ctctgaaact gatacatact
     1561 gctattcatt ataggtgata caaacaccgt tttagtatca ttaaacatgc ctactgaacc
     1621 aatgtaagca ggtattacct tatggttttg tgcaaacccc tttagcttgt ctatgcaaca
     1681 agcctcatcc acatcattga aacaagccaa agtaagatga ggtcttgttt tccattctaa
     1741 gtacttcgta ctaatttttt catcagcgat ttttttagat aaatcaaaaa gttgcttttc
     1801 cgttttttta tcataatata gctctatcgc atattgcata ataacacctc caccataaag
     1861 taaaaatacc tatctataaa aacacacaaa tacctatcat tttaatatta tattactact
     1921 gttcctcttc caattttcta tatagtgtag ccaaacctat gcctaagtct tttgctatct
     1981 tcttcttcgt ctttgtatct cttccatact tattcaacaa cttgtttatg taagtcaact
     2041 ctaattcttt aattgaaagt atcctttctt gattctctac tatccctcca actatatcat
     2101 cttcaaatat tatatttaca tccttatttt tataaatatt tccattaata ttataatagt
     2161 ttaaaatatc taatggaagc atatctttat atattattcc atcatctcca accaaattca
     2221 tcatatattc aactgcattt tccagttccc ttatatttcc cggccaatta tagttaatca
     2281 acatattcat tacattttct tctattttac ttaactgaat acctaattct aaagaatatt
     2341 tagaagcaaa cttcatcatt attgcctcaa tatcatcctt tctttcccta agaggtggca
     2401 aatttatagg aattacattc aatctatagt ataaatcatc cctaaactta ccttcattta
     2461 ctaatttcaa taagttttta tttgtagcag caatcactct tatatctaaa tctataagtt
     2521 tatttgaccc aattctcgca aattttctct cttgtaaaac tctaagtaac tttgcttgta
     2581 aaggcattga caaatctcct atttcatcaa gaaatataac accttcattt gcaagttcaa
     2641 atttacctac tttacctcca ctactagctc cactaaatgc accttttata tagccaaata
     2701 gctcactttc aaggagattt tctggaatag cagcacagtt tatcgcaata aatggcttgt
     2761 tccatctgct accttcagca tgtatagccc tagcaattag ctctttaccc gtaccacttt
     2821 ccccagttat aagtacagtt gaatttgact ttgctaattt cttagttctc tcttttactt
     2881 ttaacattgc ttctgaattt ccaattatag actctatatc actatttccc cagccactat
     2941 tcacttttcc atgtccttta tgattgatat atgtcttgtc aaatattatt attttgtcgt
     3001 attgatttat atatggatat actggaatta ttttggcatt tatattataa attttgttat
     3061 caatatcaat ttctagtaat tcatgtccat ttgaggaaga ttcaacagat actatattta
     3121 ctatattttc tattatattt ttatctatat ctaatttttt tagagctata ttgtttatat
     3181 aagatatttt gctatttatg tctagtatta taacgccttt ttcaacattt tcaatgattt
     3241 ctatcatcat atccattttt ctactgctat tttttgacgc ttcatgttct tctattttca
     3301 tagaaattaa atcgcatata tgattgacaa attttaaata attatctatt tttgcactta
     3361 tttctacttt tttagtttta tctgtactaa tagcaccaat tactcctata gttcgattat
     3421 tatattttat tggaactgct attattactt tatttaggca tttcatgtaa tgggaacatt
     3481 ctatacaaag ctgattatca catatatcca atacgactaa ttcctgacct gtctgtataa
     3541 cattgtcata aataaagccc tcagtaacaa cgctttcatt tatctttgat ttatacagac
     3601 ctgtaccact aattcttatt aatctatcgt caactatttc aatatctaca tctaacacac
     3661 tagctactgt ttcagtgtat tttatcattt cttgttgaaa ttcttttaat ttacttaaca
     3721 tagtttcccc cattagaatt taatttacca aattatcagt ttagctatat tttaacatat
     3781 agtcactctt taaaatatat ataaaacatt acttatttat atatttgcgt taaaacagac
     3841 aatattaatt tataaaaagt ttgttttata tagtaacctt aattataata tgcagacaat
     3901 aaaataaatc acaatacaat aataaaaaaa agctttcctc atataacaaa tatatttttg
     3961 acataaaaat atacattata tgagaaaagc tcaatagtta aatatttaga tagattttta
     4021 tatagacttt tttaatataa acttttttaa ttgtagactt tttttattag tttttaattt
     4081 ttattagttt tgttattggt tttattgtta taatacttat aatactgcca ttgcttcaat
     4141 ttctaaaact gcatcttttg gtaattgagc aacttgtacg catgaccttg ctggtggatt
     4201 ttctttaaaa tatgtagcat atacttcatt aatatcatta aaatccttta aatcttttac
     4261 gaatactgta gtcttaaata ttttatccat acttgtacca gcttgttcta atattgcttt
     4321 tacattatct aaagaagctt ttgttgcttc ttttgggtca gatactaact cgccagtctt
     4381 catatctatt ggaagttgac ctgatgtaga aactatgttt ccaacttgaa ttgcttgaga
     4441 atatggtcca attgctcctg gagctttctc tgtagctatt actacttttg acatgacttc
     4501 acctcctatt attaagttat atttacagga aaataaaatt tcctaaacac caatattaat
     4561 acgaaccttt agattaaact tcaaatctaa aacttcaaat aatcaatact tagatgatta
     4621 tgtttatttg tgtcttgtta acttttatta aagcaattaa cgtgccaaac tttatttata
     4681 tataaaattt tgtgaagtca ttcaaaatag ttacttctga attttttcgt aaaaaaactc
     4741 tctaaaatta cttttttatg atgtcaattt ttttctcata tctagaaatt taccaacatg
     4801 agaattcttg ttttgagaat ttacagatta aagttatttt ttaaccttaa attctgaacc
     4861 tcccatgact aaagtcacag ggttcctgct tcatagaatt ttgcataatc aagttattac
     4921 atatgaaaga actatagttg acaatcccac tattataaat aataaaacct ttacccatac
     4981 cttatttacc ctagttatca tagagcctat tgaaaatata acagctaaat ataataataa
     5041 ccaaaaaatt atcaatatct accactgttg ctgaaaatcc aaaaaactac cttttgatag
     5101 tcttacttaa caatctaatt aatctcataa taaatttaca acttataaat aaattgttat
     5161 attggccgta tctcaacaac tttctccttt cctagtttta aatcttttat agaattgtac
     5221 cacgtctaag taaattttca tactattaag tcttatttct aataaatcat tagctctcat
     5281 tctatgccca ataatgtaag atcgcagtaa ttttattgat tttaatagtt ttatttaata
     5341 tacttatatt aattattttc atatgaaatt attatttata tagggggatt tttttaatat
     5401 gagcaaaagt gaattaacag cagaaacaac agaagaaatg ttagaagtac taagtggtaa
     5461 agattatgat attgcatgtc atttacatga acttggtaaa tcattagatt gtaaaattga
     5521 accaaaaaca ggtgctcgtt cttacaaaat agtatattca actaagaaac caaaacgtag
     5581 cttatttact attgaatgta atgaaaaaaa atggagagtt aaagcaaatc tttttcatct
     5641 aaatacatat aaagatgctg tggaggaatg ctctaaaact attaaagata gtataactaa
     5701 aactcgtact tgtaagaaat gtaattcaaa gtgtattgga ggttcttcgt ttgaattaga
     5761 tggaaagtct tacctgactt gcataggaag tggtcattat tttgcaaata tggaagaaat
     5821 ggattggaaa aacctagaaa aattaattac taaagaaaat aatattatgc aggaatctgt
     5881 atagtttttt aagggtaatt attaaaatat atgagcaaca acagatactt aaataggcat
     5941 aaaaaaataa agggtataat aaggatatag aataaaaata aatgatatag cacaaagagg
     6001 ctgtctcaaa atacagataa gcctcttttt attgttgaaa tgataagaat atattcaatt
     6061 ttatacaatt ttatacaaat atactacgcc aacttaccca tttcatatgc ctgtttcatt
     6121 gccttacttc ccttaatatc acctacattc caagcaccta caccatatac tatacctttt
     6181 tccttagaac catttagaca tgatgtaaat cctctaaatc cttctattgt tctttccata
     6241 tcagccacat tttcatctgc tgctgtaacg ataaaataga aatctttatc acaaatttca
     6301 gtatatctag agcaagttct atcaatcata gttttcattt gcgcattcat agtataaaaa
     6361 taaactggag tcgctaaaac aatcacatct gccccaatca ttttttcaag aatttctccc
     6421 atgtcatctg tctgtgaaca atttgcttct ttttcataac aagcaccaca tccagtacaa
     6481 tagttaatat ctttatcttt taaaaatatc ttctccactt gatttttaga ttctaatacc
     6541 ccttgtataa actggtcaca caataaatca gaatttccac cttttctagg actagctgat
     6601 actattaata catttttact cataagatac tgcctccatt ttcttatttc tactgctctt
     6661 taatactttt tctttctcaa taataccttc ttcatatcta tcaatcttaa aatttaatcg
     6721 ttccaaagtt ttttgcattt cttctattct ttttgctagt tggtttctct gctctattaa
     6781 aagttctttt ctagctttga tagtactgct cccttgttga aacatagcta catactcaat
     6841 taatgtctca atagaaagac ctgcacctct catacacttt atgaaattaa cccactcaca
     6901 atcttcttct gtaaaagacc taattccact tttatttcta tttacaggtg gtattaaacc
     6961 aattcgttca taatatcgta aagtatccgc tgaaagctcg tattttttac taacttctga
     7021 aatcgtcata catatcttct cctcttgata ttgtatagat tgaattattt ctcaaaggtt
     7081 tctgctacat tctttaaagc atctataatt ttaatgtgtt gaggacagct ttcttcacat
     7141 tgtttacatt caatacaatc tgaggccttt ccataagttt tagtataatt attatagtag
     7201 accatctgtg ttgaaaatcc agtattaaga gcttgttttt ctgcattgta caacgcaaaa
     7261 tagtttggaa tagcgatatt ttttggacag ccttctacac aatactgaca ggcagtacaa
     7321 ggaactaaaa ttgattcgtt gataatctct actgcttcat ctataatatt atattcctct
     7381 tgtacgaatg gtttgaaatt ttgcatatat cctgtattgt ctaataattg ttccatattt
     7441 gacatcccac ttaagaccat cataacatta tctttacttg ctgcaaaacg tattgcccaa
     7501 gatgaaattg acatatctgg attgtagtca ttcaataatt tctctgcttt ttctggtatc
     7561 tttgcaagtg tacctccttt tactggctcc ataacaatta caggcttatt atgcttctct
     7621 gccacctcgt agcattttct agattgtatg ctttcattat cccaatctaa ataattaatc
     7681 tgtaattgca cgaagtctac ttctggatgc tcactcaaga ctttatccaa taattctgca
     7741 ctatcatgaa aagaaaaacc aatattcttt atttttcctt cttttttctt ttcttcaata
     7801 aacttaaagc tatcaaattt tttagctact tcataatgtg acacgcctat attgtgtaaa
     7861 agatagtagt caaaatagtc tacaccacat ttttctaatt gctcattaaa aatggtttct
     7921 tgctcttctt ttttctttaa agccattaat ggaagttttg tagctatagt aaaactctct
     7981 cttttgtgtc tttttactag agattctcta agtgctatct cactttttcc catgtgatac
     8041 acataggctg tatcaaaata tgtaaatccc ctttctagaa aagtgtctac cattttattt
     8101 aaatgttcca tatcaatagt tgtaacatca ttttcatctt ttattggtaa acgcattaat
     8161 ccaaatccta atttttttgc ctccattgtt ttacttcccc ctttaaaact tgtttgtttt
     8221 ttctctctac tttaactatt cttacaagta tataattctt gtataattat actttactac
     8281 ttggagttaa cttcaagtca atagattttt ttatttttaa gacaaatatt ataaaaatac
     8341 agttctttaa tattaatcca ttacatgttt ctttcaccct ccaattctca aacttcacaa
     8401 actccaacat gtaaagaaat atatacttta agaaattcag tccattaata ttactacaaa
     8461 tactttttag atatgtttaa aaagaaattt cacctaaaat atttataaaa gatttagaaa
     8521 taggtcttgt gtctatttta aaatatataa aacagtgata aagacttatt tataagataa
     8581 aaaatgaata gtgtataccc tttttaaaaa gttttttcct ttttatattt gtgatacaat
     8641 gttaaatata aaaaggaaaa ttttgtatta aagaaatctt atttgttgat tttgtaactt
     8701 caaactcagt atcaatttga ttttaagatt atattttatt aagtatgcta aataacaaat
     8761 ttttaaagat tgtgaggtaa aaatatgaat aagaaaaatt tagctatatt aatatcaggt
     8821 atgatgtttt tttctagttc ttcagcaatt tttgcagaca atactacaaa gcaagaaaga
     8881 ttaataggta aaacaagata tgaaacagca gtagaagtaa gtaaattggg atgggttcaa
     8941 tcaaaaacag ccattatagt taatggtaat tctattcaaa gtgcgctttg tgcaaatcca
     9001 tttgctaagc taaagaatgc acctatactt ttagtaaaca acaacagtat tgaaaattca
     9061 actaaggctg agcttaaaag gcttggtgta gataatgtgt acattgtaga tagtggaaat
     9121 agcatttctt ctaaagtaga aaatgaaata aaatcactta acataaaaat aaataaaata
     9181 gtaggaaaca atatatatga aatgtctaca aatgtactta aagaaataga taagataaaa
     9241 aagatagaaa atgttgcagt tgtaaatgga ctaaagggac tatctgatgc agttagtata
     9301 gcttctccag cagcaataca taatatgcct ataatattag tatcagatga aaacagcaat
     9361 gatagtagta taaatttctt aaacaatagg tctataaaaa aatcttacat agttggagga
     9421 aataagattt tatcagacaa tacagtaaag aaatatccaa acagagaaag aatcagtggt
     9481 tcagatagaa acaatactaa tgcaaaaatt attgataaat tttataaaga taaaaaaata
     9541 aataatgtat ttgtagctaa agatggtatt aaccaagaaa atgcattagt agattcatta
     9601 tcaataggag tactagctgc taaagaaaat tctcctataa tgatatctaa tggtagcctt
     9661 gtaaatagtc aaaaagaatt tattaaaaat aaagatatag atgaaataac acaagtagga
     9721 ggaggatata atcaaaaagc atttgaagat attttaaaac taaaaaatgt gaacataaga
     9781 aataaaacta aaaaaatatc tttaatacag gctaaagaaa tattaaaaca agctcttcca
     9841 ggaagcaata ttttagtttg ttattatgat gaagatgata atgaatatga ctgcaaagtt
     9901 ataaaggata atatcatata tgagatagaa attgatgcct actctggtaa aataatagaa
     9961 attgaaaaag atgatgactt aaatgataat aatttaggta cacaaaatat atcattggat
    10021 aaagctaagt ctatagcatt agatgctgtt cctaatggta atttaatcta ttgtaaatat
    10081 gatgctgaag ataacgaata cgaggctaaa gtaataaaaa ataagaaaac atatgaatta
    10141 gagataagtg catttgatgg aagaattgta gatattgaca tggacaatga ttctgatgat
    10201 gactttgatg ataatgataa gaataactct aatactaatg acaacagttc tattattagt
    10261 gctgaaaaag ctaaatcaat aatgctagat aaagtgcctg gtgggaaaat agtaaaattt
    10321 aaatatgatg aagatgacaa agaatatgaa ggtgaaataa taaaaggaaa aactgaatat
    10381 gagataacaa ttaatgcaat tactggtaaa atattagaat ttgaaataga tgattaataa
    10441 aaaatatata ttctattaat tattacaagc aataaagcca taaatattga aattttcaat
    10501 atttatggct ttattttatt ttttttaatt taaatccaca ttatgccatt atatatatct
    10561 atgcactctt ttaaattaaa atcttgtcca tcatcccaaa cctaataaag ttagatttta
    10621 aacaatatat tgtgatataa gtaatttgtg tagaattatt gcgtgattta ctcatgaaga
    10681 aacaataagg ttaatatata atttgactat attaagacga aaggagacat atacaatgaa
    10741 tacaatatta aaagtaaata acataactaa aacttataat aaagggcaac agccgagttt
    10801 agataaggta tcctttaatg tatcagaagg tgaatttata ggtataatgg gagcttctgg
    10861 ctctgggaaa acaactctac ttaatgtatt atctacaatt gataaaccaa caagtggaga
    10921 tattttcata aataatataa atttgaaatc gcttaatgac acttgtgctg ctgattttcg
    10981 taaggataat ttaggcttta tctttcaaga gtatttttta ctagatagtc ttacaatttt
    11041 cgagaatatt tcagtacctc ttacacttca gaaactcaat ccagataaaa tagaaaacat
    11101 ggtaaagagt ctagctgaac gatttggcat agcttctcaa ttaaataaat acccaaatga
    11161 attatctggt gggcaaagac agagagcttc agcagttaga gcaatagtaa aacatcctag
    11221 tattctcttt gcagatgaac caacaggagc tttggattct aattcagcta ctgaactact
    11281 ccactcacta caggaagcaa atgaggagtt gaaaacaaca attttaatgg taacacatga
    11341 tgcctatgta gcaagttttg caaatagaat tttgattttt aaagatggtc atatcattaa
    11401 agaattaaat aaacaacaga ataatagaca tacattttat gagttgatta ttcaagaaat
    11461 tgcaaaatta gatactaaaa gataggagga taatttaatg aacatttttt atacggcaat
    11521 aaagaatctt aaaaaaaact tttcattcta ttctctatat ctactttcag tctcttttgt
    11581 tattactgta tttttcgcat ttacttcatt ttctgttaat aagataatgc tagaaaaaat
    11641 ttcaactgat ggtagagttg aatctatgtg taatgttatt tcgatatttt tgatggcatt
    11701 tgtcatcttc tatatggcat actcaaatcg attcttcttg agacgtcgta caaaagaatt
    11761 aggtatatat gctttacttg gctatcgtaa gtttacaata ttatcgctac ttacctttga
    11821 aaatgtatta atttgtagtg gagcttttat tattggattg gtattaggag cattggcaca
    11881 taagggtatt gtctttggaa ttacagcact acttaattta tcaattgata attcacagat
    11941 accatttttt aatataaatg caataaataa aacaattatc tttatagcat tagttgtttt
    12001 agtcttagct gtatcaaatg gtaaattttt ttataagact ccacttgtaa aattgataag
    12061 atatgaaaaa agtgcagaaa aaaaattaaa gttcagaaaa attccagcct ttatggggtt
    12121 tattatgatt ttaagcgggt attgtttatc actagatatt cttcgtggag ctaaatcagt
    12181 atgggtaagt attggatttt atcaaatggg cttgataact atgattatca ttgtaattgg
    12241 tacagttctc tttatcaatt cctttcttcc atatattatg caaaatagta aaaaaaataa
    12301 acgtactttc tacacagata tcaagataat agctattcca aactttatct atcgtatacg
    12361 ttcaaactct aaagctctaa ttatgcttac attactctct gctactactt tgacagtttc
    12421 aagtgttatg gcactgtcct tatactaccc tattgcatct gtttcgcgta ttgcaccttc
    12481 agagatagaa tttagagtag aaaaaaataa acaaatagaa gatgtaaaac aaattgtcag
    12541 tcaatatacg tctaatacag atgatattat ttttacacag acaaatattt ataaggtaaa
    12601 atcttcatca aaagaccttc catttgaata tgaaattggt acatctaaga ataatttatc
    12661 taatagtagt tcttctaaag aagttatttc aagagaatca ggatttgaat gtatatcttt
    12721 ttctgattat tatactttat taaaggcaca aaatcgaaca aagctactaa aacaacttac
    12781 ccctttaagg gataatgaat gtattttaat gaaatatcaa ccaaataatg atggatatag
    12841 tgaaaatgga acgatttatc cattacttgt taatggtaaa gaaacacctg tagctgtaag
    12901 ggaaacatca ttaaacaatg ctatttcttt tgccaatagc gttggaacgc ttatcgttaa
    12961 tgatagtata tatgagcaaa tagcacaaaa taaattacca atgacaagca ttttaagtat
    13021 taatggtaaa tcaattaaaa ataatgaaaa tttatatagt aatatttcag attatttaga
    13081 tggaagccct tatttacaag gatattctca caggataaat gatttattat caattaatag
    13141 ttctacattt ctgttaatcg gatttcttgt ggtattattc tttattgctg ttggaagtat
    13201 tttatacttt aacaatatat cagcaatttc agattcaaca tctgattata agattttaca
    13261 gaaaatgggt tatgataata tacaaattaa aaaaattatt aaaaaacaag tattgccatt
    13321 ttttaatatt ccgtttttac ttggtatatt ggactgtata tttgctacct tggtttataa
    13381 gactgctttg atgcagaatt tacttggaaa ttcattttca caatacatac ctgtgattat
    13441 tgcaattttc ttaacttctg tagtgtattt gatatattat atacttacta ttaaatcctg
    13501 ttgtaaaatt gtattaaaaa attaatattt accaaatcgt caagcttaga ggtgtgacga
    13561 tttggtatac ttcatttata agtatttgat ataatttaaa attatagatt cttaggtttg
    13621 atttagtcat aaaatcaata taattaactc ataaggaggt gtataagatt gacaaatttt
    13681 aatttaaaaa tactcttttt aggaaactac attctatcac ttctcttcat gggaatatct
    13741 acaagcactg aaacttatat gaatatccaa agcattattc ccttaataat ttcaacatta
    13801 ttactcatag acatcacata tataacctct tgtaatgtcc gagaaaataa cattatttca
    13861 ttgttttgtg gattactagc actagacagt tggtatttac tcttatcatt tgtaaccaca
    13921 ccaataggca ctattatttt taaaatatta agcacagtta tagtgtatgt ctctattaaa
    13981 tttatatttt tatttttgtt tcaaggtagt gcatacaatt tccgtaagat aacagacatt
    14041 ttattgctta tcacctgcat tggtgcaatt gtaggagcat ttatttctaa cagaacgtat
    14101 gcttgtatgt acggaataca gtttgttgtt agtataataa gctttttcta tattattttt
    14161 tatcatttga aaagggttgt ttttgtcata aaaagcgaaa aaaagcctat atcgatttca
    14221 gtatttataa caattatagg attccttact tattattttt ttacactaag aatatcaaat
    14281 catattggta attttggaat ctatctaact gtattggtat tttttatgag tattcatggt
    14341 attgcactta aagaaaatga tagttttcct atttcaacag tttttaacat aaaacaaagg
    14401 gctatatttc tttttataag tattttagta attagcttgg tcgtactatt tgccaatggg
    14461 aatttttcta taatattaat agctataaat gttttattta tgcttatttt cctctgtaac
    14521 attattttgg agtttaacct tagagataaa aaaaataaag tagttaaaaa tagcaagtat
    14581 acttttgcat tagaaaaatt acaacaagaa gaacagctaa aaacagagtt ttctaatttt
    14641 ttacatgatg acatattaca gaacctgctt tcaataaaaa acatgatgag taagtcatat
    14701 cgcccagaag ttcaagatat tatatatgaa acacttaaca atctaaatac atgtataagg
    14761 aagcaaatgc aagactatca ccctgttatt ttaaaaaatt taacagtaaa agaaaattat
    14821 cagaatttaa ttgaaagtat ttcttcatcc tttccacaaa aaaatatttg tgtttcattt
    14881 aagtgttctg atactttatt cttagttgaa ccatatgata tattgatata tcgactgatt
    14941 aaagaattat tgacaaacgt atataaacat tcaaatggta gccatgcatg gatagcttta
    15001 gcactagaaa aagatataat aaaattaaat attagtgata atggtgtggc aaatacatct
    15061 tttaaaacgc ctttagatat aactaaaatt acttttgata caataaaaca caaagggatt
    15121 atctcaatta aagaacaagt agaaaatcta ggaggagcga taaatatttc tgataatata
    15181 cctcatggag tttgcgtaca gattacaatt cctatgaaag gagatggttc ttataaatat
    15241 tttgttagtt gatgaccata tgctttttgc taaaagcttg tcaattgcac tagaagaata
    15301 caaagaaatc gaacagtttt attccacaca ggacatccat aatctttcag aaatgattca
    15361 agataaaaaa atagacattg tcttaatgga tataaacctt agaggcttaa gtgaatgtga
    15421 tggcttaaaa gtagcttata atttattaga gactaattca actattaaaa taataatctt
    15481 gactggatac gacctcccag tttataaaca tgaggctaaa aaaataggta tctatggatt
    15541 cctaaataag aatattagcc ctgatagttt aattaattca ttattaaaag tctatcaagg
    15601 ggtaacttgt tttccatctg aaaatactga agtaattatt gaagatttaa ctactatgga
    15661 gaaaagaatt ttacagctta tctgtagtgg taaaaaaaga aaaataattg ctgatgaatt
    15721 gtatataagt gaaagaacat tatcaaatca tttacaacac atatatgaca agctagatgt
    15781 ttcatcatca gttgaggcta ttacaaaagc tattaaaatg ggttatattt ctccagtata
    15841 ttaaaaaaag tgagtgtctc aaaataaatc ttctaactca taaagcactc tcctttgtat
    15901 taaatcaaat atattttcat catttaaaca attaagaaga ggcttctctc tattttgaga
    15961 caactccatt ttaatacact atttatacat aaacatattt taaccaacta aaaagcaact
    16021 aatatctcaa tacatatctt cataacacat atcaccataa ttacaagcag ataaagaact
    16081 ctcctcattt aaatacgact caaattcata cccaaacaga attgttggtt ctatatatat
    16141 ttccatatct gtatatctcc atcttcttac ctttgcctct ataaccttgt aaaaatcttc
    16201 taaactatac ccttctttta atcttttatc tataagtgcc ttagttatac tcaaatcagc
    16261 tttaaaattt tttcctgatt cactatttaa ttttcttata ataatttcat gtatgctttc
    16321 tggattttta taagtagaat cttcagtcaa atcttcatcc gaattttcaa aggtattttt
    16381 atttaaattc tttttattta aattattttt taataattct tttttctttg tctcattttc
    16441 tgtttcatac ccagtatgaa ttatcaaatc ataatctttg tctatactct cttgatttat
    16501 acggctgtat atgttggtat tactgttctt aaacaactca tttctatgtt tatatttcat
    16561 tgttttttgg ttattgttaa tagttgcatc aatgtatttc tctatatttt tgaaaatatc
    16621 accattcttt gttacaattt ctgcattact actttcagaa caaacataac tatatacaga
    16681 acaacaattt ccctttactc ccctacttat taatctcaat atacctaaat ctataaattc
    16741 atttacaagt ctacttattg tagatataga tacatcaagg tcagatgcag ctttccttac
    16801 tgtcatcata aattgtcctc ttggcagagt tccttttaat ctttgtatat tttcttttct
    16861 cattacatag tgattaaacc ttattctatt tacatctctt ttttcattaa aatataatac
    16921 tgctgaattt aattgataaa aactattact catattccca ctcctccaat ttaaatatat
    16981 aatttgttgt aaagttttta gatttttaaa taacttatta aatgattatt tttattaaaa
    17041 agtattcttt gatattttca ttttaaag
//
LOCUS       DAEORZ010000089        16858 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEORZ010000089 DAEORZ010000000
VERSION     DAEORZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.089"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            499..834
                     /locus_tag="KRH39_002513"
     CDS             499..834
                     /locus_tag="KRH39_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512411.1"
                     /translation="MINLFKKEDLNLLLEYPKEVVENVDNVINILDESYGDNRKITDN
                     GGYVCIIEDIKEIEYLKSHILNGLIEEFSDVIYESEVDIYNSTLYLLSSDYSITIISK
                     NEETEHLLK"
     gene            898..1359
                     /locus_tag="KRH39_002514"
     CDS             898..1359
                     /locus_tag="KRH39_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG8512412.1"
                     /translation="MDKKKIPAKRVDIVSLKLVKESSVLYETRKISNPYDAYRLVKNF
                     LVDSYREKFVVVCLDTKNQPVSIEIVSIGTVNSAMVHPREVFKVLVLSNASKIICFHN
                     HPSGNTNFSKEDEVITKRLQKCGEILGIELVDHIVVGDDDKYFSFKENFKI"
     gene            1500..1793
                     /locus_tag="KRH39_002515"
     CDS             1500..1793
                     /locus_tag="KRH39_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512413.1"
                     /translation="MFKKENRYMTREIAENLPIEITILLWDLIDNLDIEKDYLQIFEI
                     NPIGLGVVEIVHRQEVPEYEASIYIQNDLIKYKLKIYAIDSIKYSTMMFSNEY"
     gene            1820..2416
                     /locus_tag="KRH39_002516"
     CDS             1820..2416
                     /locus_tag="KRH39_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512414.1"
                     /translation="MINNKSEIINKLFFNELKELIDKCNNIEDKTVYMIEKIFISIED
                     EEIKNYLIRNNCKLQELVNRYKKLELLNIDEAIFFAWYNLNINTISIDKASQYYYELT
                     TSNYIEVESHLVYTNEIDLREYAKDELDTMLDMEYHIDRLLDKDMIIDMWLNNTSKED
                     VIEEILMSDDIEDILDISPEYAFTTTSGIEYRYSEKEE"
     gene            2612..2827
                     /locus_tag="KRH39_002517"
     CDS             2612..2827
                     /locus_tag="KRH39_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HBG8512415.1"
                     /translation="MVDILGEEIVIKLYKYYRGQQITFPMKLYSNEYVERYIEKNYRT
                     KTLKDMCRELGYTEGWIKQLINKYKLK"
     gene            3056..4582
                     /locus_tag="KRH39_002518"
                     /pseudo
     CDS             3056..4582
                     /locus_tag="KRH39_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427584.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4876..5214
                     /locus_tag="KRH39_002519"
     CDS             4876..5214
                     /locus_tag="KRH39_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512416.1"
                     /translation="MEQYSFSSELIDVMVNASNDLNRVYEKITSSGTFSGLQGTFDTL
                     YEYGLMLEDLLEVMDLSLLGGNETKEIIQNIAIDLKNISDEDNIADKALDLMGNIGEF
                     TYYIETNYSK"
     gene            5349..5594
                     /locus_tag="KRH39_002520"
     CDS             5349..5594
                     /locus_tag="KRH39_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512417.1"
                     /translation="MRSVTVEDGEERFAIEKIYVKSLKRYEVRICLYRDCKDRMNKLI
                     PRPVDISPDKLSTLILVGIKAGILDDDFKQELIKGLS"
     gene            5892..6083
                     /locus_tag="KRH39_002521"
     CDS             5892..6083
                     /locus_tag="KRH39_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512418.1"
                     /translation="MKYTLKYGLGQLKFDVKIRLEDILRLSSAGEDNDEVRLAERSRE
                     INDIIGKLYLRPSETKKKI"
     gene            6172..7085
                     /locus_tag="KRH39_002522"
                     /pseudo
     CDS             6172..7085
                     /locus_tag="KRH39_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7266..7547
                     /locus_tag="KRH39_002523"
     CDS             7266..7547
                     /locus_tag="KRH39_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512419.1"
                     /translation="MELKTIRITFTVSSETSEDDLRKLCKDYMGDEYVVNDEGDFEIS
                     FYLNTVLGYNEVLYFMELIHMELEKSITLLGIRYKGIGGLQMKHILQEE"
     gene            7554..9215
                     /locus_tag="KRH39_002524"
     CDS             7554..9215
                     /locus_tag="KRH39_002524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512420.1"
                     /translation="MIRNCSKIIKNYGFKINSKNHVFDSNGNEICNFVPIVLGQYMIG
                     DGRDRRTVIVLMGISEDGNQLETINIDVSDIEHLKWVKNSWGFQYKVHSKMKNQFLYL
                     IEVLVINGTTKKLSSQIGWVKDNGKYAYLHTRGVIGESDKEVDIGDNLSNYFLPSEIV
                     DINRACKASLNLLEVADYEVTVPLLALVYLSPLLQAIKDVGKMPEFVVWLFGTTCSRK
                     TSLARVFLSHFGDFTNRLPATFNDTYASIEIKANAIKDSLNLIDDYAPKQTKKQKDAQ
                     DEIAEKAIRAYGDRIARGRVNSSIISQKQYIPQGMLLMTGENLIRGHSTVARLVALEL
                     DKDSVNLKLLKEMQDSVSLLGESMRGYIEWLLHGMNKDSSDIGGILIHNFDLYKEELE
                     KIEYTHTKGGHGRSIESCAWLLVGISSMLSYFEDKKVISEVEFNQYIQFAKDTMVNVL
                     IKNNNITKESSPIDEFLYTIKEAIDSNSIKISTLVDGNKLNDNDDDTYGYKDDKYFYF
                     HPDKTYSYVQEIQSKSGNYISLTKRGLIKLLREHSIIKVDSDGSP"
     gene            9350..9787
                     /locus_tag="KRH39_002525"
     CDS             9350..9787
                     /locus_tag="KRH39_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512421.1"
                     /translation="MTIRINLDGENILENVDNKFYGLRARVDFDLDLLDEDVIDIMDR
                     ILTLDVNPSRMFITKGRVDIFYQPQKEISIESKLGYMKLINEFIDFIESQEIVEMKFL
                     GWNLNGDKIVESQANDSNISFSKEIFNPDTVYLDGLIEGYVNL"
     gene            9894..10809
                     /locus_tag="KRH39_002526"
                     /pseudo
     CDS             9894..10809
                     /locus_tag="KRH39_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10839..10994
                     /locus_tag="KRH39_002527"
     CDS             10839..10994
                     /locus_tag="KRH39_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512422.1"
                     /translation="MTETYKISIGRKDLYSKDYKAVERIDYEKRYIVNISRSLVDILS
                     ILIEEGI"
     gene            11457..11921
                     /locus_tag="KRH39_002528"
                     /pseudo
     CDS             11457..11921
                     /locus_tag="KRH39_002528"
                     /inference="COORDINATES: protein motif:HMM:NF019149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            11907..12614
                     /locus_tag="KRH39_002529"
                     /pseudo
     CDS             11907..12614
                     /locus_tag="KRH39_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            12747..13811
                     /locus_tag="KRH39_002530"
     CDS             12747..13811
                     /locus_tag="KRH39_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4297 domain-containing protein"
                     /protein_id="HBG8512423.1"
                     /translation="MSKIEYYMNLPYDLSGSMSKNRFRNELLWGLKKMYELYKTNEDF
                     VVVFDYRCDIEVHLENKFEFYQVKTQNNSGTYTINKLTDKKKGSKSVVGNLYSLKYDI
                     NMNEIDDVYVAIVSNAPLNDGKKTYNNSHEVKIKTIDSSAIEKIKLSIKNDLNIDEDI
                     NLENLFFIRIGLDLIEPQKTLIGETAIFFEDVFGRECSKVKSLYNVLYEEIYSKSAYE
                     LDINTYENIVSKKGINRDRMDIILKSYANNTDKSVELAKYSINEWCRNNFSRRLSLIK
                     SLTTIVSKLSESSILKDIENCILSYIYNNIDKLQVSDKELIEIISEIVEEKRPIEVSK
                     TDIEALILLTLKKYEEGVYE"
     gene            13804..15477
                     /locus_tag="KRH39_002531"
     CDS             13804..15477
                     /locus_tag="KRH39_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512424.1"
                     /translation="MNSLFVNSLYIFEYESKMAKKIDFKHGINILTSDEKSGNDVGKS
                     VLLKSIYHTLGADSIFDHMWNKITKTYILNVTIKNKTYYVYRRDNLFKIYNTNFKKIF
                     MTNNRDELSNYLSDLYSFKVMLPKRENEELELAPPVYSYLLNYIDQDHLDGSKFSSFN
                     SLTQFPNYKENVIYTHFGLFDEEYYKAIKELEILKSEEKNKKKEREVTLNMLNKIKSY
                     LGNLDAPESIEVLNIELNKTKEEYSNIVINLQKIKNNLMKIRNEKVDLELNIKEIELT
                     RRNEMKKLKVKNVCPSCSHEIEDIDSKFIINNQIEDLIILKDELESEIIKCNRVIEKK
                     EEEYQSLLDKLHEYESNMKINDDSISNVLKHKAYIDTKDDIVLDLEIIDKKIKEIQQT
                     IEPYDKKIKEYNSLKKKANAKYEELMKKAKKDFGLEEIDENKIKNIKNNFIARGSNRA
                     ITTIMWYLTLLAVKYEFNENAIKFPLILDSPNNVESDEIKEESLFKYIFKNTKTDTQL
                     ILSSLGFNEMQFSEFKFDNIIKLENSKYNLLNKEDYYNNIDILNKIFEN"
     gene            15582..16544
                     /locus_tag="KRH39_002532"
     CDS             15582..16544
                     /locus_tag="KRH39_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512425.1"
                     /translation="MKDRFNLKRKSIQSLMNKNVDVYSCKDCIDDFIESNFKIYRSYY
                     ISDDDILEIMDGTGEDYKDVIADMLPDKAHIKSGEFGEITATEIYKSNIQTTEIYIVD
                     KLSHTRKEDTKVAAHKTDILIIEKTSEGFIIHSSEVKTKITDSDFNPIEAMIEGVNDD
                     IVTRVAETLNWIQRKLKTEKKFDELKFIKQVIESIENQNVENEYNGAVFIDESKLDYE
                     INKNIYCDVKLSKSQKGKYDNELKSIGCVIDGDKIRFDSVNIDDINKLNINSRKRNSI
                     EKLYNKSQRKLEHIDDIKIQIVTFDDINKHCLDMYEKIIHVGRI"
     gene            16544..>16858
                     /locus_tag="KRH39_002533"
     CDS             16544..>16858
                     /locus_tag="KRH39_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512426.1"
                     /translation="MSINNNINKIRNLDIQLAKYDCISRMSAKETSKHYMRLYFKFIK
                     LLELYEDSSLNINNGEYLNLANDLIIFSKYDMSSRFKGIDEERNLVYIAVIYYILGYR
                     FEC"
BASE COUNT         7448 a         1367 c         2968 g         5075 t
ORIGIN      
        1 agaaataaat aatttaatat tttatttaca agtttagttt agaaatatat tagataattc
       61 catattaata ccatacgata taagtaatat attaactaga ctatatgggt ttgaggaagt
      121 acaaaatatt gaagatatat caatagatca aacgatagaa ttaacagata atatagatga
      181 aaatatatac aaaatagatc aaatattaga aaatagatca aacctatatg tggatgggtt
      241 atatgatatg ctagaaggta taaagttaga gagtgtacaa gataatccga ctgtatttga
      301 agaagagtat gtaaaacttc aaaataaata aggaatctag taaaatgtaa taattataat
      361 tatatattat aattttgaaa tatacattaa atagatatca tactaaagag gaacatacaa
      421 aaaataacac attgagatgt tggtagagat actaacatct ctttttttag ttcaaaaaat
      481 tataaggagg aaattaaaat gattaactta tttaagaaag aagatttaaa cttattatta
      541 gaatatccaa aggaagtagt tgaaaatgtg gataacgtta taaatattct tgatgaatca
      601 tacggagata acagaaaaat aacagataat ggtggatatg tgtgtataat tgaggatatt
      661 aaagaaatag aatatttaaa atcacatata ttaaatggat taattgaaga gtttagtgat
      721 gtaatatatg aaagtgaggt tgatatctac aattcaactc tatatttact atctagtgac
      781 tatagcatta ctataatatc taaaaatgaa gaaactgaac acttacttaa ataagcatat
      841 acattgataa agtgtatatg cttattttaa ttttgagaaa caaaggagga aataatcatg
      901 gataagaaaa aaatacctgc aaaaagagtt gatatagtat cacttaaact agtaaaagaa
      961 agtagtgttc tatatgaaac tagaaaaata agcaatcctt atgatgctta tagattagta
     1021 aagaactttt tagtagatag ttatagagaa aagtttgtag tagtatgttt agatacaaaa
     1081 aatcaaccag ttagtataga aattgtaagc ataggaactg taaattcagc gatggtacat
     1141 ccaagagaag tatttaaagt attagtatta agtaatgcat ctaaaataat atgctttcat
     1201 aatcatccat caggtaatac taactttagt aaagaagatg aagttataac taaaaggcta
     1261 cagaagtgtg gtgaaatatt aggtattgaa ttagtagatc atattgttgt tggagacgat
     1321 gataagtatt ttagctttaa agagaatttt aagatatgag agtcttagct taaagaaatt
     1381 aggctaagac tttttctatg ttctaaacta attgaaataa attagtttta agttaagccc
     1441 gaaaagccct tgaaaaaata atatacatat aaataattaa actaaatagg agttgtaaaa
     1501 tgtttaaaaa agaaaataga tatatgacga gagaaatagc tgaaaatcta ccaattgaaa
     1561 taactatatt actatgggat ttgatagaca acttagatat agaaaaagat tatctacaaa
     1621 tatttgaaat aaatcctata ggattaggag tagttgagat agttcataga caagaagtac
     1681 ctgaatatga agctagtata tacatacaga atgacttaat taaatacaaa ttaaaaatat
     1741 acgctataga tagtattaaa tacagtacta tgatgttttc aaatgaatat taactaataa
     1801 ttaaaggaga acgagtaata tgataaacaa taaatctgaa ataattaata agctattttt
     1861 caatgaatta aaagaattaa tagataaatg caacaacata gaagataaaa ctgtatatat
     1921 gatagaaaag atattcataa gtatagaaga tgaagaaata aagaattacc taataagaaa
     1981 taattgtaag ttacaagagt tagtaaatag atacaaaaaa cttgaacttt taaacataga
     2041 tgaagctata ttctttgctt ggtacaactt aaatataaat acaatatcta tagataaagc
     2101 aagtcaatat tactatgagt taacgacaag taactatata gaagtagaaa gtcatttagt
     2161 atatacaaat gagatagatt taagagaata tgctaaagat gaattagata ctatgttaga
     2221 tatggaatat cacatagata gattattaga taaagatatg ataatagata tgtggttaaa
     2281 taatacatca aaagaagatg tgatagaaga aatattaatg agtgatgata ttgaagatat
     2341 attggatata tctccagagt atgcattcac aacaactagt ggaatagagt atagatattc
     2401 agaaaaagaa gaataaaata aatatatgtg ttgaaaaaat acgggcttta agggcttata
     2461 ccttaaagta tgtataaaaa atacaataga ttttacaata gaaaatcaga atgaaacttt
     2521 ttctaatact aattattatt aatgagtatc agaaaaggag gtggagtaat gatcaagaat
     2581 aatagcgact atcaaaggga tatatgcaga tatggtagat attctaggtg aagaaatagt
     2641 cattaaatta tataagtatt atcgtggaca acaaataaca tttcctatga agttatactc
     2701 taatgaatat gtagaaagat atatagaaaa gaattataga acaaaaacat taaaagatat
     2761 gtgtagagag ttaggatata cggaaggttg gataaagcaa ttgataaata aatacaaatt
     2821 aaaataaata ccctagttat tgttaaatta ttcaataaaa aatatgattt tattacaaaa
     2881 atttataatt tcgacaagat gaatgttgta ttatatcaac gaaagatatt aaatttattc
     2941 atatcaaata taaattatca aactttataa ctaagttatc taataagaga cattagtaca
     3001 aagttcatta gtaagtgaac ataaagaatt aaggcaatgg agagggagat tgaaaatgac
     3061 aaagtttaat tttataggaa atgaaattta tataacagaa gaaagaaata ggtataatag
     3121 cataagaata gaatatgaaa atatagcgaa taaagcaaga aaagaattta ttaaagtata
     3181 taggtcatgt aatgaaaatc tagatgatgt aattaataat gcttatgctc aaggggcatc
     3241 aattatttta aaatcaatta agtgtacact agatcgactt atagaaaata aattttataa
     3301 tataagcgaa gagttattta ttgaacaata ctgccaaaga gttgtagaaa tttgggaatc
     3361 tgcatacggt ataatcaatg actagtatat gaatattgta ttagatgaaa ggcaaaagga
     3421 agagtataga caactaagga aaaattcaag agctagatgg caaggtggag gttttgggat
     3481 tcaaggggct ataaaaggag ctacacaagc aggagcaatg aatatggtta caggagttgc
     3541 acattctgca tttaatatga taagtaaaat aggtacgagt ataaaagtaa ataaacaaaa
     3601 atctaaaata tttaatgatc catcgacatt agatgtatta tctgaagtaa tttatttagc
     3661 cattttagat attaaatact catatataaa gtttttagaa aataatgcag gattacagtt
     3721 tgggtatata catgaagaag aggaagaaga agcaaatgtg atactttcaa atataaaagg
     3781 cagaaattta gatgaagaag aaaaaataaa tttaattaag ggacttatag atttaaatcc
     3841 atacatggag tctctataca catacatagt agataacttt ggtgatgaaa atatggaagt
     3901 ttctaagatg gctagttatt ttggttttaa tatagagcca tacaagctta gtttagtaga
     3961 gaataaatta attaatttag aaactaacac agaagaagaa acaatattag ctaagcagct
     4021 tatattaaaa gaagtaaata gattaggtat taatagtaag gtaaatggaa ttgaagagtt
     4081 agaccgaaaa ttaaatcaat ttgatataga agcgaggaca gtagataaca tattgtttga
     4141 aacaagagaa aaagctaatt tagcaagaca agaaaaatta gatatagaaa gtataataaa
     4201 aaatttagat gaaaacaatg aagaaaatct agttaatatt aaaaaagaga ttgaatctag
     4261 aaattttaaa acagaaatag ccgatttata tatagataga ataaataatc atattaaaaa
     4321 tttatattct gacactataa atgaagcaga acaatatgag gataataaaa cgaattttaa
     4381 aagtacgtta ataggatcag cgtttattgt acctctaggt atatactttt ttggtaatgt
     4441 tgggattata ttaaagatag ttataggtat attcttatta tccgcagtat ctgcattatt
     4501 tgaatcatat aaaaaattaa aggcaagtaa gtattcatta aaacaactta aaaagttaaa
     4561 aaagtcaggt aaaataatat aattatcatg aggaatcaaa ttgaaaggta tatttaataa
     4621 aacattgaca aagattataa taagttcatt aatatttgga actgtatgta gtagtatagt
     4681 ttatctaggt atgagaaatg aatttaacaa taaattagtt ttttagaaaa agagatacta
     4741 gacttaaaaa aacaaaacgt agaattatct aataaagaat tagaaaaaga atatgataat
     4801 tcagacgtta aatctgaaga tgttaaaaaa gatagagaga ttactaatga ggtaaaaaaa
     4861 gaatatgaaa aagaaatgga acagtatagt tttagtagtg aattaataga tgtaatggta
     4921 aatgctagta atgacttaaa tagagtatat gaaaaaataa caagctcagg aacatttagt
     4981 ggattacaag gtacatttga tactttatat gaatatggat taatgttaga agatttacta
     5041 gaggtaatgg atttatcttt attaggaggt aatgaaacta aagagattat tcaaaatata
     5101 gctatagatt taaagaatat atctgatgaa gataatatag ctgataaagc attagattta
     5161 atgggtaata taggggagtt tacatactat attgagacta actatagtaa ataattaaat
     5221 attaataata atagccttgc tacacatgac tgtagcaagg tattattttt gtaaagaaag
     5281 gaagatttaa tttggctaaa caaaataagg ctcaactatt tgaaaaagaa actaaatatt
     5341 gtaagataat gagaagcgta acagttgaag atggagaaga aagatttgca atagaaaaaa
     5401 tatatgttaa gtcattgaaa aggtatgaag ttagaatatg tttataccgt gattgtaagg
     5461 acagaatgaa taagctaata ccaagaccag ttgatataag ccctgataaa ttatcaactt
     5521 taatattggt aggtataaag gcgggtattt tagatgatga ttttaagcaa gagctaataa
     5581 aaggtttatc ttaggaggta tagaatgtcg atagaagaat ttactagtgg agtaatgttt
     5641 ggaatttcta tatttggtac agtattaaag aagttattag ataataaaaa atagtacata
     5701 ggaggtgtta ttatgattgg atttataata ggaactatga taggagttgg attatgcatt
     5761 agcatagaaa ttatgatacc aaaaaataca atagtatatt aacctatatt taaactgtta
     5821 atataatcat atataattta tttgagtcct taaagcccag aaaagtttta tatgaaggag
     5881 aattttataa tatgaaatat acactaaaat atggattagg acaattaaaa ttcgatgtaa
     5941 aaataaggtt agaagacata ttaagattgt catctgcagg agaggataat gatgaagtaa
     6001 ggttagctga aagatcaaga gaaattaatg atataattgg aaagctatat ttaaggccga
     6061 gtgaaactaa gaaaaaaatc taatagttat gaagaaagta aaaataaatc taaaagtaaa
     6121 tatgatagag ataaaattaa ggacatatta gaagaagatg agttgaatgt aatggaaaag
     6181 tatgtatttt catttgaatc aggaaataat aagctattta aagagtattg gaaagacaga
     6241 aagaagacag atataccatt aataacaaca atatttttta gaactatgtt aagattaaag
     6301 gatacatata gacatgattt gtataactta tgggaagatt gtaatctaac aacggaagaa
     6361 tttaaggaaa atatatataa atcacaaaat gaatctatag gatttattaa atttaaagtt
     6421 gaaaaaatga tagaagaaga aaaagaaagg ttagggaaaa gtattaataa caaatataaa
     6481 agcaatagaa aaataacaga attgcagaat attgatattg ataatatagt aaaagaattg
     6541 attatatctg ataatcctga aaataaagaa atctatcaaa agatgataat tattacaaaa
     6601 tcatttttta agataattga atatagaaga aaattatcat taagtaatca aacagtaact
     6661 gataatttaa tggaagaggt aaaaaaacta tataatacat taccaaaaga atcgagatat
     6721 gatgcagcat ataagaatct aagaaagtta aaagaagata ttgaatttaa gataaaagaa
     6781 atagataatt ataaggagat atataaaaga gaagttataa aaaattgtaa attagttagt
     6841 gagtataaaa aaaaagaaga aaaactaaag aagtctggtt atatagaaga atcagaaaaa
     6901 atggcaaaaa aatatataga tatagaaagg caatttaatg agagtatgag aaaaaaatgg
     6961 atatatatat agatatgtat ataagacgta aggacttaga aatagaagaa atggatatga
     7021 aaaaatatga agatgataat ataacgaatt cgatgattaa attagcagaa gagattaact
     7081 cttaattagc ataaaaatag tacacagtta agtgtgctat tttttttatg aaaatgaaaa
     7141 aaaattggat aatggtttgg ttatttttta ataaaaaatt agcttatatt taatttcaga
     7201 aggaggtaag gaaataaaaa tacgggctat aagggcttta ggacaactta gtaggaggaa
     7261 caaaaatgga acttaaaaca ataagaatta catttacagt aagtagtgaa acaagtgaag
     7321 atgatttaag aaagttatgt aaagattaca tgggtgatga gtatgttgtt aatgatgaag
     7381 gtgactttga aatatccttt tatttaaata cagtacttgg atacaatgag gtcttatact
     7441 ttatggagtt gattcacatg gagctagaaa aaagtattac tctattagga attagataca
     7501 aaggaatagg tggactccaa atgaagcata ttctacagga ggaatagagt aagatgatta
     7561 ggaattgtag taagataatt aagaactatg gttttaaaat aaacagcaaa aatcatgtat
     7621 ttgatagtaa tggaaatgag atttgtaact ttgtaccaat tgtattagga caatacatga
     7681 taggtgatgg aagagatcga aggacagtta ttgtattgat gggaatatca gaggatggta
     7741 atcaactaga aactattaat atagatgtta gcgatataga gcatttgaaa tgggttaaga
     7801 atagctgggg atttcaatat aaagtacatt ctaaaatgaa gaatcaattt ctatacttaa
     7861 tagaagtatt agttataaat ggaacaacta agaagttaag tagtcaaatt ggatgggtta
     7921 aggataatgg aaagtatgca taccttcata ctagaggtgt gattggagaa agtgacaaag
     7981 aagttgatat aggtgataac ttaagcaatt actttttacc aagtgaaata gtagatataa
     8041 acagagcgtg taaagcaagt ctaaatctat tagaggtagc agactatgaa gttactgtac
     8101 cattattagc attagtatat ttatcccctt tacttcaagc tattaaggat gtaggaaaga
     8161 tgccagagtt tgtagtatgg ttgtttggaa caacttgttc aaggaaaact tctttagcaa
     8221 gagtatttct gagtcacttt ggagatttta ctaatagatt acctgcaaca tttaatgaca
     8281 cctatgcatc tatagagatt aaggctaatg caataaagga ttcacttaac ttaatagatg
     8341 actacgcacc aaagcaaact aaaaagcaaa aagatgcaca agatgaaatc gcagaaaagg
     8401 ctatacgagc atatggagat agaatagcaa gaggtagagt aaactctagt ataattagtc
     8461 aaaagcaata tatacctcaa ggaatgttgt taatgacagg agagaatctt ataagaggac
     8521 actcaactgt tgcaagatta gtagcattag aattggacaa ggactcagtt aatttaaagc
     8581 tactcaagga aatgcaagat agtgtaagtt tgttgggaga aagcatgaga ggatatatag
     8641 agtggttgtt acacggcatg aataaagata gtagtgacat tggaggaatt ctaatacaca
     8701 actttgattt atataaagaa gaattagaga aaatagagta tacacataca aaaggtggtc
     8761 atggtagaag tattgaatca tgtgcatggc tgctggtagg tatatcatcc atgttatcat
     8821 attttgaaga taaaaaagta atatctgaag ttgagtttaa tcaatatata caatttgcta
     8881 aagatactat ggtgaatgta ttaattaaga ataataatat cacaaaagaa agttcaccaa
     8941 tagatgagtt tttatacacg ataaaagaag caatagattc taactctatt aagataagta
     9001 ctctagtaga tggaaataaa ttaaatgaca atgatgatga tacatatgga tataaggatg
     9061 ataaatattt ttattttcat ccagataaga catatagtta cgtacaagag atacaatcga
     9121 aatcgggaaa ctatataagt ttaacaaaga gagggttaat taaattatta agagaacact
     9181 cgataattaa ggtagatagt gatggatctc cataaaagta tactattaaa gtaaaagatg
     9241 gaactgacta taaagagaaa agatctaggc tattacgatt agctataaat acaatagaga
     9301 aattataaga caataagaga taaatggtga tatagaaaga ggtaattata tgacaattag
     9361 gattaattta gatggagaaa atatattaga aaatgtagat aataaatttt atggattaag
     9421 ggcacgtgtt gactttgatc ttgatttatt agatgaagat gtcatagata ttatggatag
     9481 aatattaact ttagatgtaa atccatcaag aatgtttata acaaaaggta gggtagatat
     9541 attttatcaa cctcaaaagg agatttctat agaatctaag ttagggtata tgaagttaat
     9601 taatgaattt atagacttta tagaatcgca agagattgta gaaatgaagt ttttaggatg
     9661 gaacctaaat ggagataaga ttgtagaatc acaagcaaat gacagtaata tttcatttag
     9721 caaagaaata ttcaatccag atacagttta tcttgatgga cttatagagg gatatgtgaa
     9781 tctttagaaa aagctaagtt agaagtatgt agttctaata ggagctgcat acttacttaa
     9841 tacaagatta ttgagaagtt aaaaaaagga aattattatg gaggagaact tatatgaact
     9901 atattacaac acaaggattt gatacaataa aaagggataa gatagaaata aaaagaatgg
     9961 tgtatgcttt agagaatgag ggtgattcgt acaatgttat tgaagaatgt atatgtactt
    10021 tagaagaaaa tatagatgta aatgatgatt ttgtgataga agtagaaaat gtatttagtt
    10081 ttgttattga tagaataatg gactttataa aagagaagaa tttgccaaaa aacaaaatag
    10141 aacatgtaag agcttatata gatgtatttg aaaatccaac ttcaaataca aaaatagtaa
    10201 agtctataga tatatatgaa gattatgata aaagatatat tgataataag ttggataagt
    10261 tatttaaaag catggataat atgataaaac ccgaatttgt tccagtaata gatggtaaat
    10321 ttgatatacg tggaagtaag gatttgaaag agtataataa aaaattaatg ttagacatag
    10381 taaagcaaaa aacttatgaa gtaaataatg gaggaataag aataaataaa ttatctgcaa
    10441 tattaaaatt aggagataaa agcatagatg gcataagcaa aatgatgaag gatgtggcaa
    10501 atctaaagat aaaaccaaaa tcagtatata agtgaagata cagttgttgt aatgtatata
    10561 tgtaataaga gttgtattgt gcttaatata gaagagtatt ccaagatact gtcagatttt
    10621 gttgactaca taaataatat agaagatatg gacttatatt tagatgatag cattttatat
    10681 ggtgactata aaataaattg tggagtagac gatagagata taattgattt taattatcaa
    10741 agctttaatt caaaagtaac tttgtttgct ggacatatag gaaacgagat aatacaagct
    10801 gcattataga aatatacaat cgtagagagg atatagatat gacagaaact tataaaatca
    10861 gcataggaag aaaagattta tattctaaag actataaggc ggttgaaaga atcgactatg
    10921 aaaagaggta tatagttaat ataagtagaa gcttagtaga catactttct atacttatag
    10981 aagaagggat ataaattata gatatggcta catacttagt gtgtagcctt taatatagac
    11041 ttaaattagt atatttaaac tttggaggat ataatatgaa atcaaagaaa gcagtagtat
    11101 atgcaagatt tagtttggac aatcaaaggg atgagtcaat agatgctcaa ttaagggcaa
    11161 tcaatgaata tgaagataag aataatataa aaatagttaa tcagttcata gatagagcaa
    11221 aatcagcaac atcagataaa agaccagaat ttcaaaatat gattaagttc tgtgaagcag
    11281 atacaacagg aatatctatg gtaatagttc ataagctaga tagattttct agagataagt
    11341 atgattcagc tatgtataaa cagaaattaa aagtaaaagg aataagagtt gtaagtgtat
    11401 tagagaacct agataattcg cctgaatctc taatattaga atcagttata gaaggtatgg
    11461 cacaatacta tagtgctaac ttagcaaggg aagttgctaa aggtcaaaaa gaaaatgctc
    11521 ttaaagctct acacaatgga ggagatgcac cattaggata tgatgtagca cataataaaa
    11581 cttatacagt taatgaagaa gaagcacaag cagtaaagat tatatttgat agatatatta
    11641 aaggatattc atatagtaag ataatagatg aattaaatga tttaggatat aagactaaaa
    11701 gaggtaataa atttggaaag aatagtttac atggaatatt gaataatgag aagtatacag
    11761 gagtatatgt atttaataag actcagagaa aaggtgtaga tggaaaaaga aatggtcatg
    11821 agcaaaagag tgaagatgaa ataataaagg tagaaggtgg tataccagtt ataattgata
    11881 aagaggtatt tgaacaagca caagagatga tacaaaaaag aaagaaaact ccaggagcac
    11941 ataaagctac tactacatat ttattaacag gattaattaa atgtggagaa tgtggacatg
    12001 catttcaggg gaataagaga aaagactctt atggtaatga atatgtttca tatagatgtg
    12061 gatgtagaaa gcaaaaacga gattatctag aagagtttat attacaagaa cttgaaaaga
    12121 acatattaaa tgatgaggca ataccagctt taagtaaagc attgaatgag aagttgaatt
    12181 ataagaatga aagtaatgca gagttactta aaaacttaga tcagaaacta gagaaggtaa
    12241 ataaagagat aagtaatata ctaaatgcaa taatgaatgg tatagtaagt agcatgttaa
    12301 aagataagct tgatgagcta gaacaagtaa agttaaactt agatttaaaa atgaatgaat
    12361 taaagataga aaataaagtt gtagatggtg taggcgtaac agaagaacaa ataagaagta
    12421 tgttctcaag atttaagtaa tttgtgctag agaggaacat acctgaatgt aagaaattta
    12481 taggggacta tataaaggaa gtaatcgtat ataaagatca cgttgaagtt gtattcaatt
    12541 tggtcttttc ttttgtcgat gatgaaatag ctcataactt acacattact gtatcaagag
    12601 taaaagtaaa ataaaaacat taaatataaa tgttaattta aatgatatta tggatatatt
    12661 taacaaataa gtagtgttac atgatataat atcacatgta tatgaaaggg ataaaattta
    12721 tactattagt attggtggtg aaatgaatgt caaaaattga atactatatg aatttaccct
    12781 atgatttatc aggatcaatg tctaagaata gatttagaaa tgaactactt tggggattga
    12841 aaaaaatgta tgagttatat aagacgaatg aagattttgt agtagttttt gattatagat
    12901 gtgatattga agttcattta gaaaataaat ttgaatttta tcaagtgaaa actcaaaata
    12961 attcaggtac atatacgata aataaactta ctgataaaaa aaagggtagt aaatcagtag
    13021 tagggaattt atatagcttg aaatatgata taaatatgaa tgagattgat gatgtttatg
    13081 ttgcaattgt atcaaatgct cctttgaacg atggtaaaaa aacatataat aattctcatg
    13141 aagttaaaat aaaaacaata gatagtagcg ctatagaaaa gataaagtta agtataaaaa
    13201 atgacttaaa tatagatgaa gatataaatt tagaaaattt attttttatt agaattggtt
    13261 tagatttaat tgaaccacaa aaaactttaa ttggtgaaac tgctatattt tttgaagatg
    13321 tttttggaag ggaatgtagt aaagtaaaat cattatacaa tgtcttatat gaagagatat
    13381 atagcaaatc agcatatgag ttagatataa atacgtatga aaatatagta tctaagaaag
    13441 gtattaatag agataggatg gatattatat taaaaagtta tgctaataat acagataagt
    13501 cagtagaact agcaaaatat agtataaatg aatggtgcag gaataatttc tcacgtagat
    13561 tatctttgat aaaatcatta actacaattg tatctaaatt aagtgaaagt agtattttaa
    13621 aggatataga aaactgtata ttgtcatata tatataataa tatagataaa ttacaagtat
    13681 cagataaaga gttaattgaa attataagtg agatagtaga agaaaagagg cctatagaag
    13741 ttagcaagac agatatagaa gctcttatct tactaacatt aaaaaagtat gaggagggtg
    13801 tttatgaata gtttgtttgt gaacagtcta tatatatttg aatatgaaag taaaatggcg
    13861 aagaaaatag actttaaaca tgggattaat atattaacat cagatgaaaa gagtggtaat
    13921 gacgtaggaa aatcagtact attgaaaagt atttatcaca ctttaggagc agatagtata
    13981 tttgatcata tgtggaataa aattacaaaa acatatatat taaatgttac tataaaaaat
    14041 aagacatact atgtatatag aagagataat ttatttaaaa tatataatac gaattttaaa
    14101 aaaatattta tgacaaataa ccgtgatgaa ttatctaatt atttaagtga tttatatagt
    14161 tttaaggtaa tgctgccaaa aagagaaaat gaagaattag agttagcacc tcctgtatat
    14221 agttatttgc ttaactatat agatcaggat catttagatg gatctaaatt ttcatctttt
    14281 aattcattga cacaatttcc taactataag gaaaatgtaa tttatactca ttttggactt
    14341 tttgatgaag agtactataa agctataaag gagttagaaa tattaaaatc agaagaaaaa
    14401 aataagaaaa aagaacgaga agttactttg aatatgctaa ataaaataaa aagttactta
    14461 ggaaacttag atgcaccaga aagtatagaa gttttaaata tagaattaaa taaaactaaa
    14521 gaagaatatt cgaatatagt tataaacttg caaaaaataa aaaataattt aatgaaaata
    14581 aggaatgaaa aagtagattt agaattaaac ataaaagaaa ttgaattaac aagaaggaat
    14641 gaaatgaaga agttaaaagt taaaaatgtg tgcccaagct gttcacatga aatagaagat
    14701 atagattcta aatttataat aaataatcaa atagaagatc ttattattct taaagatgag
    14761 ttagagtcag aaattataaa gtgcaataga gttatagaaa agaaagagga agaatatcaa
    14821 tcactattag ataaacttca tgagtatgag tcaaatatga aaataaatga tgatagcata
    14881 tctaatgtct taaagcataa ggcatatata gatactaaag atgatatagt tttagattta
    14941 gaaataatag ataagaaaat aaaagaaata caacagacaa tagagccata tgataaaaaa
    15001 ataaaagaat acaacagtct taagaagaag gcaaatgcta agtatgaaga gttaatgaaa
    15061 aaggctaaaa aagactttgg attagaagag atagatgaaa ataaaataaa aaatataaaa
    15121 aataacttta tagcaagagg tagtaataga gcaataacta ctataatgtg gtatctgaca
    15181 ttgttagcag taaaatatga atttaatgaa aatgcaataa agtttccact aattttagat
    15241 agtccaaata atgttgaatc tgacgagata aaggaagaat cattgtttaa atatatattt
    15301 aaaaatacta aaacagatac tcaattaata ttatcatcgt tagggttcaa tgaaatgcaa
    15361 tttagtgaat ttaagtttga taatataata aaacttgaaa atagcaaata taatttatta
    15421 aataaagagg attattataa taatatagat attttaaata aaatatttga aaattagtat
    15481 aggaatcgtg ggtatgattt ctgtgaatcg tctccaccat tgaaatccac gaaaacacat
    15541 tgatatttta tcaatgtgtt ttttaagtaa tagggggaaa tatgaaagat agatttaact
    15601 taaaaagaaa aagtattcag tcattaatga ataaaaatgt agatgtttat tcttgtaaag
    15661 attgtataga tgattttata gaatcaaatt ttaaaattta tagaagttat tatatatcag
    15721 atgatgatat attagagatt atggatggta caggagagga ctacaaggat gttatagcag
    15781 atatgttacc agacaaagcg catataaaat ctggagaatt tggtgaaata acagcaacag
    15841 aaatatataa gagcaatata caaacaacag aaatatatat agttgataaa ttaagtcata
    15901 caagaaaaga ggatactaaa gttgctgcac ataaaacaga tattttaatt attgaaaaaa
    15961 cctcagaagg ttttataatt cattcttctg aggttaagac taagataact gattcagact
    16021 ttaatcctat tgaagcaatg atagaggggg taaatgatga tatagttaca cgtgttgcag
    16081 agacactgaa ttggattcaa agaaaactga aaacagaaaa gaaatttgat gaattaaaat
    16141 ttataaaaca agtaatagag agtatagaaa atcaaaatgt agaaaatgaa tacaatggtg
    16201 cagtttttat agatgaatca aaattagact acgagataaa taaaaatatc tattgtgatg
    16261 ttaagctttc taaaagccaa aaaggcaaat atgacaatga attaaagtct ataggatgtg
    16321 tcatagatgg tgataagatt aggtttgata gcgtgaacat agatgatatt aataaactaa
    16381 atattaatag tcgtaaaaga aattcaatag aaaaattata taataaatct caaagaaaac
    16441 tagaacatat agatgatatt aaaattcaaa tagtaacttt tgatgatatt aataaacatt
    16501 gtttagatat gtatgagaaa attattcatg tagggagaat ataatgagta ttaataataa
    16561 tataaataag attaggaatc ttgatataca attagcaaaa tatgattgta tatcaagaat
    16621 gtctgcaaaa gaaacatcga agcattatat gcgattatac tttaaattta taaaattatt
    16681 agaactatat gaagatagta gtttaaatat aaacaatgga gaatacttaa atctagctaa
    16741 tgacttaata atattttcta aatatgatat gtcgagtaga tttaaaggaa ttgatgagga
    16801 aagaaactta gtatatatag cagttatata ttacatattg ggatatagat ttgagtgc
//
LOCUS       DAEORZ010000090        16673 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEORZ010000090 DAEORZ010000000
VERSION     DAEORZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16673
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.090"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            87..1475
                     /locus_tag="KRH39_002534"
     CDS             87..1475
                     /locus_tag="KRH39_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512427.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFTPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            1503..2456
                     /locus_tag="KRH39_002535"
     CDS             1503..2456
                     /locus_tag="KRH39_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG8512428.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIETLTKEKQAGVSLG"
     gene            2516..2740
                     /locus_tag="KRH39_002536"
     CDS             2516..2740
                     /locus_tag="KRH39_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512429.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFISLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            2733..3245
                     /locus_tag="KRH39_002537"
     CDS             2733..3245
                     /locus_tag="KRH39_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBG8512430.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            3277..4131
                     /locus_tag="KRH39_002538"
     CDS             3277..4131
                     /locus_tag="KRH39_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBG8512431.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            4665..5459
                     /locus_tag="KRH39_002539"
     CDS             4665..5459
                     /locus_tag="KRH39_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8512432.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            5563..6369
                     /locus_tag="KRH39_002540"
     CDS             5563..6369
                     /locus_tag="KRH39_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG8512433.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     AIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            6431..6952
                     /locus_tag="KRH39_002541"
     CDS             6431..6952
                     /locus_tag="KRH39_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG8512434.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            7367..9454
                     /locus_tag="KRH39_002542"
     CDS             7367..9454
                     /locus_tag="KRH39_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8512435.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLVEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            9469..9756
                     /locus_tag="KRH39_002543"
     CDS             9469..9756
                     /locus_tag="KRH39_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8512436.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            9783..11054
                     /locus_tag="KRH39_002544"
     CDS             9783..11054
                     /locus_tag="KRH39_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBG8512437.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            11112..11711
                     /locus_tag="KRH39_002545"
     CDS             11112..11711
                     /locus_tag="KRH39_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG8512438.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            11716..12621
                     /gene="dapA"
                     /locus_tag="KRH39_002546"
     CDS             11716..12621
                     /gene="dapA"
                     /locus_tag="KRH39_002546"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG8512439.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVNLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            12727..13359
                     /locus_tag="KRH39_002547"
     CDS             12727..13359
                     /locus_tag="KRH39_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBG8512440.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNVRKI"
     gene            13836..16343
                     /locus_tag="KRH39_002548"
     CDS             13836..16343
                     /locus_tag="KRH39_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG8512441.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNQKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVLQGRTSPCPFC
                     TNSYLKKDDTYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDES
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
BASE COUNT         6349 a         1877 c         3061 g         5386 t
ORIGIN      
        1 gaaaacgata ttggatttaa gtaagtttgt ctcataaaat gaaagtatat caattgataa
       61 tactttaaaa taagaaggga gaaaaaatgg aagataaatt ttatgcaaaa ggcaacggaa
      121 ataacggata tattaaaaat cttgaagttt gttcctttaa taacttagat ggaacttgtg
      181 gaatgtttca aatggctctg tacaaaagag atgaaaaata ctatttatat ggatgctgtt
      241 ttggaggaaa taaaaaaaat ggagtaatga ttagcgatat tacagaccct tataatccac
      301 aatttataaa acattttcaa atgttagacc ctaaagagta tcctacaaca acaactccca
      361 aaattcaaat agcagatgat ttaatgatag tagcaatgag ttgtggaagt ggaccaggag
      421 cacttgttga ccaagctaaa ttagcaaata ttaagtgtga agcaggaatt agaatataca
      481 gtttaaaaga agacccttta aatcctaagt ttttaggata ttgggattgt ggcttaaagc
      541 atgtaatggg tgttcataga ttcatgtaca atggtggaag atatgtacat ttatcaagtg
      601 attgtgttgg ctttgaaggt ctgatttata gggtcataga tataataaat cctactaatc
      661 cagtggaaat aggtaaatgg tggagaccag accaatatgc agatggatat ccaaatagaa
      721 cttttgatgc aggagcacct cattgcccag aatttatgga taaaggatgg cttcatggac
      781 ctccatttgt aagagacgga aaagcatatt gtggttatgg aggagctggt ttagttgtat
      841 tagatgttga agatttaaca agaccaagat gcttaggtga attgccattt acgcctgcat
      901 tttctagtag acttgcaggt gcaagaactc atacagcatt accattgcca ggaagagatt
      961 tagtcgttgt tcaaaatgag ggagaaagat tccagttctt taaaccagat aacattacag
     1021 atgttcaagc tatgaataat atacatatgg ttgatgttag tgacccaaca aaaccaacat
     1081 taattgctca atttccatat cctgaagttc caaaagattt cccttatcct aactttaatg
     1141 ttgcaggatt aggaaaacca gggccatttg gtccacataa tcttcatgaa ccaatggata
     1201 ataagccatg gttagagcaa agaggagata gagtatattg ctgttatttc catgcagggc
     1261 taagggttta tgatgtatca gacccatatt atatcaaaga gctagcatat tttataccac
     1321 caaatccaaa taaaacacca gaagaatctt atttcccagg attcccagga ccacgcttgg
     1381 cagtaacaga agatcttatc gttgatgata gaggctacat catcatagat gctttagatg
     1441 atggattcta tatattaaaa atgaaagaag attaaataaa tactaaagag ggagagttga
     1501 aaatgaaaat actaggtatt tcttttggaa caaaaaatgg aaataatgac acaatgtgta
     1561 aagttgcact taaaggtgcc aaagaagcag gggctgaagt agaatttatt caaatgtcat
     1621 ctattaacat taaacattgt actggatgtt gtgcgtgtgt taaaacttta ttatctggga
     1681 aaggtagtat gtgtgtatta aaagatgatt ttgaatggtt gcttgacaaa atgaaagatg
     1741 cagatggtat agtagtttca gaccctattt ttgaagaagg ggcatcagga ttattccata
     1801 ctataatgga ccgttttgga cccagagcag atagaggaaa taacattata ggaacaaaag
     1861 ttgcagaagc tataggagga aaaattccag attcaagaat gcttaatgat aaagtaatat
     1921 catttatggg aataggaggt tctgactggg gtacaagagt tcaatgtgaa catgctatgc
     1981 tagctctcat accaatgtgg aaagttatag ataatgcttg gttcccatgg gcaaaggaat
     2041 tagtaatgga tgatacaagg cttgttcaag ttcatcaaat tggtttaaat atagttgaag
     2101 cagcaaaaga ttttgaaaaa gcttcatatc aaggagaaga aggtgtatgt ccacattgcc
     2161 acaatagatt attctacctt gagccaggta ctaaaaaggc aatttgtgct ctatgtggta
     2221 tagttggaga gttagatact ataagtggaa aaactatatt tagttttcca gaagagcaat
     2281 taggtcatgc tcatgatact ttaccaggta agtttataca tggtgatgat attaagaaaa
     2341 tggaaggtca ttatgcagaa gttcgtaaaa cacaagaatt taaagaaaga aaaacatctt
     2401 ataattttat agagacatta actaaagaaa aacaagctgg agtttcatta ggataataat
     2461 taaaatacta agactctttt ttaagggatg ttagatgtta taggaggctt attgtatgaa
     2521 tgaaaagcca agttttaaac aaattgaagt tattttagca gttatttttt tggtacttac
     2581 atattacaag gttagattaa ctccagcatt tattgttata tttatatcac tatatttaag
     2641 ttattttgca gtatctatga ttgtaaatca aatagcaatt aagctattgg agataaggca
     2701 agctagggaa gctactaatt acaatatagg aaatggatag aaagaaaaaa gaaatattaa
     2761 cactgtctat ggggattgct ttactacctc ctttatgggc agtattagca ccttatatag
     2821 gaataaaaac tggagctgta gcactaatct gtgcaggatt atatgtaact aatggtaata
     2881 aacaaaaaga tggattaaaa ataatgtttg gtttttggtg tggagattta tgggcagtgt
     2941 tagccatttt aattatggga tatatgaatt tcaatcaaaa cctagaatta tttttaacac
     3001 ttagtatatt aggttttttt gcagttgtaa ttgcatcctt atttgaaaaa ataattttcc
     3061 taccatcatg gctttgtggt tgggctatag gtctaactat tatgacaggt gaaaatatta
     3121 ttaatttaca agatatttgt attcaaattg ctgtagctat ggcagtagga gtatggtatg
     3181 tgggtgcagg tgtagatttg tttacaatat atattttgag taaagatagg aaaaagggaa
     3241 attgaaagca tgtgaatata ctttgattat aggagaatgg ctatgggttt tgaatttaaa
     3301 ataatgagaa gtttaatata tgtaggactt gccaaggaag aatatagacc taagctaatg
     3361 gactggttat atcgtcacca tattccagat agtattagca cttttggacc atattgtact
     3421 aaatatgcct tttatcaagc atatcctaca ccaaatgaag gtgagcgttt tggtgcacgt
     3481 aagatgcaac taacagaaca ttattggctt gtagatgaac atatgcctga gatggcaaat
     3541 agaattatga cagaatatat gcctatggat gttctacgtt ggcaagggtg tataccagat
     3601 gtagaaaata aaagggttca tgaaaatgca gaaagtggag atgcaggacg tgcagtaggt
     3661 ggagataatg gatgtccacc atttatattt gcctttgttc caataaactg ggaagaagac
     3721 tttagaggaa aaggacgtac tgtacaagat ggaccaaact atcgttggca atttatgatt
     3781 aagtatccag atggtatctc taaagaagaa ggagaaaaat ggttctatga tgaggtagtg
     3841 ccatacttta caaactgttg ctatgttaat cgttttgtca gtagtaaaat aatgattaat
     3901 tatggagcaa ctgcttttga ccgtgtgtca gaactatggt ttgaagggga agaagaatgg
     3961 tataaagctg tggttgaaga gacaaagtcg tttattaaaa aaccagaatg ggcacaagaa
     4021 gaggagttcc catatttaaa accacaattc aatatcgcat cagtattctt aggtgatata
     4081 gcaactatgg atgcatactc acagtatcgt ggatatatac caatgagata aaaattatag
     4141 tttgtgataa gtagaggagg catttactct tactggtaga agaagactta ttgtatcctt
     4201 aaaagtaggt gaatatatag ttttatattt aaatttatat aaaactaaga ctaataaata
     4261 tgaaacaata gaattagaat aatttgataa tatttgttaa aagagcattt agaatgtgtt
     4321 taaagattaa gatattcctt ttatggtagc agatagttga aataataaaa ctgttatcat
     4381 gaaaggaata tttttatgta taggaaattt ttaaaaataa aagttaaata tgtttaagaa
     4441 catcttaaag tgaaagaatc attgttagaa atatgtagac aatatagtat ttataacgta
     4501 tcatattaac aagtttacac ttggattatg aaataaaaag agtaattcca aaggttgtaa
     4561 atactatgct gactttgata cttcaaagtt taggtgattt tgtaattgtt aatagaaata
     4621 cgtttaatga gcataaagaa tttatctaat aataggagaa tataatgtat acaattggac
     4681 aagtatctaa atttttagga atatctagag atacactaaa attttatgag gacaaaggat
     4741 tagtaaatcc taagaaaaat gatgaaaatg gatatagaat atataatcag gttgatatct
     4801 atgatattgc aacaataaac ttttatagag aaattgatat agaaattaaa aagattcaag
     4861 aaataagaaa aagtaagagt ataaacaatt tagaactctt actagaagag aaggaacaaa
     4921 tcatactaaa agagatagaa tataaaaagc ttctcttaaa aaaggttcag ttagtcaaag
     4981 aagattgtga caaaatcaaa acatattttg gaaaatacac aataaaagaa atgaagcctt
     5041 tggaagtaaa aggtaaaata aaacatgtta ctaactatga ggaatatgaa gccttaaaag
     5101 agaatacaaa caacttaaaa aaagcagtta cattaactga tttaagaaga gtaataaatt
     5161 ttaatgaaga aggtataata gaagaaaaat ttataattgt aagggaagta gatgactaca
     5221 acaaaatcat agaaggtgaa gttctatctc atcccaattg tatttatact attattgaag
     5281 atggcagatg gtcaactgga ggagaaaata ttgataataa agtggaagac agattaagag
     5341 aaatagcaat agaaaatgaa tgtgaattac ttggaatagt ctatgttaat atattgctta
     5401 ctacttatga agaagggatt gagcgtatat ttttagagat ttatgcacct ataaaataat
     5461 ataaaagcat tgacctggga cttactcata ggtttaaact catgatataa gcagttataa
     5521 cttatatagc aattaaatat taatttgaaa tgaggattac ttatgagaac agagaaatca
     5581 ttaaaaaggc ctatagccag ttttattatt ctaacaaata taattttttt accacttttt
     5641 ttattggtag tagtgactat aatgttaaaa cttccaacag tgatatctga cattgcgttg
     5701 tgtatatcag cgtggtcatc cacttttgca tttatgatat tatttaaaaa aatttatcca
     5761 ggtaagaaat ttttagtaca cgtaaaagat agatttagaa ataaacttaa attttcaaca
     5821 gtatctataa taattagtat acaagtccta atagctgtag tagttatatt tatattagca
     5881 tctaaaaatc atggaataat gcctaacttt acagtttcat catggggggc atttatatat
     5941 ttatttttaa aaaatttatt tgcaggatca ttaggtgagg aattaggttg gagaggattt
     6001 gttcaaaatc atcttcagaa aagacattct cctttaaaag catccataat agtaggtttt
     6061 tggtggggga tgtggcattt accaatatgg tttacaacag gattcaaagg gcttgattta
     6121 ataaaatata ttatattttt tatgattgca attatatcaa tcagtattat aatgacaact
     6181 ttttataatc taaataaaac tttgataata cctattgcaa tacatcaact atttaatttt
     6241 ttaataggga taattaatgg agatttaata gagcttataa attactatgc aatgttatat
     6301 ttaatatttg caattgcatt aatagttata aatccaggaa atgtattata taaaaatatt
     6361 agagagtaat atatatcttt atggtaagat ataaagaaat tattagaaga acatccaagg
     6421 aggaataaac atggttttac tgattgttga tgcacaaaag ttaataacaa acgagaggtt
     6481 atataaattt aatgaatttg tagtaaatgt agaaaattta attgatacag ctagaaaaaa
     6541 taatatagaa gtaatatatg tacgtcatga tgatggagtt gaaaatgaat tgactaaagg
     6601 taaaaatggc tttgaaatat acgaaaaatt taaaccatgt aatggagaaa aaatatttga
     6661 taaaaaagta aatagtgctt ttaaagaaac tggtctatta gaatacctaa caaataatgg
     6721 tgaaaaagat attattattg ctggtctcca aacagattat tgtattgatg ccactataaa
     6781 atgtggattt gaacatggat ttaatataat agttccagct tattcaaata caactgttga
     6841 caattttttt atgtcagcag aacaatcata taaatactac aatgaattta tatggaatga
     6901 aagatatgcg aagtgtattt cattagagga aacacttaga aaaatgaaat aaaaatagag
     6961 tatgaattgt atatgtacat aagttgattt cataaaaaag gatgttaaat tccaagttaa
     7021 aaattagatt aaatatatta tattatagat aaggcacatc aattttattt taaattaatg
     7081 tgctttttaa ttgtagtaat ttatttataa tcaatatgat ttttattatt taaatcaatt
     7141 tctcttgagt ttctttgtct aaagaaactt tttttattta taaaatctac agttatatta
     7201 tagtatctat tttagagaag tttaattttt gtatcaatct aaaacaaaag ttatacttaa
     7261 atgaaattga aaacgataaa attctcaaat aaaataagta ttgtggaggt gatgaaagaa
     7321 tccaatgata atatgaagac taatcttaat aagaaagtag gggttaatgg tagtggacaa
     7381 agaaattgta tcaattctcc agattttatt aaatagcaca ttgattacaa ctaatggatt
     7441 acaagaggag tctaattcat caaagagaca aattacttat agaataaata aaatcaatga
     7501 catgcttaaa gtaaaaaatg taccacttat atgtttaaga gctgataaag atattatcgt
     7561 tagaaaagaa acaaaaaaag caattaaaga aattttagaa aaaaactatt ctaagaatac
     7621 ttattatttt agtaagacag aaagactttt atatatgtat ttaatgcttt ttattaacat
     7681 ggaggatatt tcaattaatc attttataaa ttctataaaa gtaagtaggt ctactgtaaa
     7741 tcttgatttc aaggatttga taccagagct tgaaaagaag aacattaaag ttaagaacaa
     7801 tcgtataaat ggatattaca tagttggcaa tgagatggaa attaggagag tgcttattaa
     7861 aaacattata gaaactttgt ctaatgagaa aagttcaaga gtatttgata tttttattaa
     7921 agaatataat ttagatagtt ttgaagaggc taaaaaaatt atattaaagc taataaaaaa
     7981 atatgaaatt acttttgttg aagatagatt agtagaattt atatatatat ttatctttct
     8041 aaaggcaagg atgtattcaa ctagaatagt tactcaggaa aatgtagata ttcctaatat
     8101 ttcagttatg agttcaatga aggagtataa atttgctaag gaattattag aagtatatgg
     8161 gagtgaagat aagataaagc ctcacaatat aatgtatatt agtgcttgga ttttaggtat
     8221 atcagtagga aattatgaag aagatacaga agataaggat gtaatttcaa aaattgtaaa
     8281 taaaatgatg acaaagtttg gatatttaag cggagtttac tatataagcc aagaaaaaat
     8341 atttaaacaa ttatattctc attttagacc tgcctactat agacttttat ttaaattacc
     8401 tatctataat ccgatttgtg aaaaagtaaa agaagaatat aaattagttt atagaattgt
     8461 aagtaatgca atgaaagagt tttgcggatt atttggagaa gaaattcagg aagaagagtt
     8521 agcatatctt acaatgcact ttgcaacact tttttcaaat aaaaaagaat ttgatggttt
     8581 tagaaaaaga atagcattaa tagtttgttc aaatggcgtt ggtagttcag ctattttaca
     8641 tgcagaactt acaaacttat ttcctgactt acactttata ccaatgtcgg aatcagttgg
     8701 attagaaaat ataagtgaac ctgtagatat tgtatttact actaattata acgcagaggg
     8761 tttaaaaaca gatgtaccag taattaaagt aagccctgtt atgaccacaa aagaaaaata
     8821 taagattaca cgtgaagtgt atattcaatt aggtgatatt tttttaagac aacctaaaat
     8881 agatgaggtt atggatataa taaaaaaata tgcaaatgta acctctgaaa gtattctttc
     8941 aagtgagcta ttagcttatt ttacacagat agagaatttt acatctaagg aaggagaggg
     9001 gcctatgcta agtgagatta ctaatgaaac actagtaaaa ataaaggtaa aagctaagaa
     9061 ttgggaggaa gctattagac agtcagcttc tgtattagtt gaaaataaca aagtaactga
     9121 agggtatgta gatgctatga ttaactcagc aaaagcatct ggaccataca tagtaattac
     9181 aaaacatgtt gcacttccac atgctagacc agaagctggg tcaaaagaga ttgcaatagg
     9241 cattgctact ttagatagtc ctattgagtt tgggaataac gaaaacgacc ctgtaaaata
     9301 tgtattttgt ctaagtgcaa tagacaataa tagccattta cgtgctatgt ctgagctagt
     9361 tgaactattg gaagaagatg agttttttaa ggttttagat agtgctaaag aagctaaaga
     9421 aattattgat tttataaaaa cacacgaatt ataaaggagg atttaattat gtataaaatt
     9481 ttaatcgcat gtagagcagg agttggctca agtttaatgt taaaaataaa gactcaacaa
     9541 attattaagg aaaataactt tccaattgaa gtagagcatg gttcattgga ttcactaaat
     9601 ggattcaatg gagatgctgt agaaacatta attgatgttg ctgaagaatt aaaaaataaa
     9661 aatttgaagt ttgatataat tgggattcac aatattgttg accgtaatga aataaagaca
     9721 gcactagaaa aattccttgc ttcaaaagag ggataaattt aagatgataa ggaggaatat
     9781 ttatggagtt tattattagt ttgctgagta cacctgctgt tttgcttggc gtagttgcat
     9841 gtataggatt gattcttcaa aagaaatcag ctgttgaagt atttacaggc tcttcaaaaa
     9901 cacttatcgg ttttttgata tttggtattg gtgcctctgc tatgacagga gcacttcaaa
     9961 atttcaataa actgttccaa catggctttg gaattactgg tgttatagct tcaccagaag
    10021 cagctacagc acttgcacaa ggtacatatg gttttgcagt ttcatgtaca ttaattcttg
    10081 gattcatatt aaatttagta tttgcacgta ttacaaagtt taagaatatt ttctttacaa
    10141 caggtcatag tttgttcttt tcttgtgtat tagttttaat tatgaaagca catggatttg
    10201 ataatacaat cactatttta ataggtggaa ctatcttagg gtttatgtct gctgcactac
    10261 ctcaattctg tcaaccattt atgcgtgaat taacaggtgg cgacgaacaa gctattggtc
    10321 actttaacat gattggttat ggtttatcag gttatattgg gcgtttattt tcaaaacata
    10381 aagatgatac aacagagact atagaatttc ctaagtggtt atcaatattc agagacttct
    10441 taatgggtct atccatagtt atgttaatct tattttatat tgcaacatta aaagctggtc
    10501 aagcatttac tcaagaaatt gctggttcaa cacattggtt ggtttttccg cttatagagg
    10561 catttacatt tgtggcaggt atgtcaatat tgatgtcagg tgttcgtatg ttcttagctg
    10621 aaataactgc agcatttgta ataatttcag aaaagtatat tccaggttca cgtcctgcgc
    10681 tagatgttcc aaccgtattt ccatatgcac caaatgcagt tattattggg tttatatcag
    10741 catatgctgc tggtctatta gcaattgcta ttatggcatc atttcctagt atatttcctg
    10801 tagtaattat tccagctgct cacatatgtt tcttctcagg aggtacagct gcaatatttg
    10861 gcaatacttc aggaggatgg agaggggcta ttgctgggtc atttgttgtg gggttattac
    10921 ttgcattttt accagttata ctatatccag tttatgggac tcttggtata gaaggagcaa
    10981 ctttccctaa tattgattac aacgtagttg gttcgatatt acataatatt ttacaattta
    11041 taaaacctat ttagactaaa ttatataaaa gtatagtcat agttactatg atttcaagga
    11101 ggatacatct tatggaaaat acattatctg aatttttaga taatatatct aatgaattgt
    11161 cagaatttat tgcctcaata gatgagacat ctataaataa agcatgcgaa ttaattcttg
    11221 aagctgaaaa aaatcacgga cgtgtacatg tgactggtat tggcaaacca ggacatgtat
    11281 cgggatatat ttcatcattg ctttcatcta ctggaacgtc agcatatata ttgcatggta
    11341 cagaagcagt acatgggtca agtggtcaag tagttgaagg agatgttgtt attgcaatat
    11401 caaatagtgg tgaaactcaa gagttaaaag caactcttaa gactctaaaa gtaaatggag
    11461 caaagataat tggtgtaagt ggtaatgaga gttcattttt aaaaaatatt tctgatatat
    11521 tcctgtttgc aggtgttaaa caggaaggag attgtcttaa taaagctcct cgtgcttcaa
    11581 ttttagctga aacaataata ttacaatcat taagtgttgt attacaatat gctaaaggtc
    11641 ttaatacaca gcagtatttg aagtggcatc cagcaggaag tcttggaaag tctattagag
    11701 agggaatata attttatgaa atataaaggg attatatgtg caatgataac accatttgat
    11761 gaaaatcaaa atattaattc acaagcaaca tgtcaattaa ttgattattt gattgaaaag
    11821 ggaatttatg gattatttat tttgggcact aatggtgaat gtcatgtatt aacagatgat
    11881 gaaaaagttg aatttgcaaa gattgttatt aatcatacaa ataatcgtgt acctgttttt
    11941 gttggaacag ggggtaattc aactcgtgaa gttgtaaatt tatctaagag aatggaagcg
    12001 attggagcaa gtgcattatc tattattaca ccatactttg ttacaccaac acaacaggaa
    12061 ttaatcttac attacaaaac tattgctaat tcagttaatt tacctattat aatgtataat
    12121 atgccaggta agactggaat taatattgaa ccagagtcag tacgtgaatt atcaaagata
    12181 aagaatattg ttggaattaa ggatagtagt ggtaaacttg acaacatgaa agcttatatt
    12241 gaagcaacga agaatgagaa ttttagtgtg ttttcaggct cagattcact tattctggat
    12301 agtctaaaag ctggtagtca aggagctgtt gcagcaacaa caaacttcct cacagagatt
    12361 ggtatctcta tctatgaaaa ctttatgaaa ggggatttgg aagaagctca aaaagctcaa
    12421 agtagtatag aagaacttcg acgtatactg aaacttggaa caattccatc tgttatgaag
    12481 aaaactattg tcttgaatgg aattaatgtt ggaactgcac gacttcctgt aactgaacca
    12541 actggggaag tacttgaaga aattaaacga gtagttgaaa attataggac aatacttaat
    12601 aaaagtagtg agaatagata atataaatag ggaaattaca ctggtatata attaatatat
    12661 atattataaa acatgactaa aattaatgaa taagtaaagt ggtttgatga gtatagaggt
    12721 gataatatga aaaagatgaa tataaccaaa agaatgtcag aatgtggggc tcttgcaatt
    12781 gtaagagaag aaaatcttaa ccgtgcatgt gaaattgctg aaggatgtat aaaaggtggt
    12841 ataacagtta tagaaatgag ttatacccta aataatgctg gagaaataat tcaagggctt
    12901 aataaaaagt atggtgaaac actttgtgtg ggagcaggaa cagttttgga tagtgaaaca
    12961 gctcgtcatg ctatattaca tggagctcaa tttattattg caccaaatta caatgaaggt
    13021 gtagcgaaga tttgcaatag atatcagatt ccttatgctc caggatgtac atcattgact
    13081 gaggcagtag atgcactaag tttaggagca acttttataa aagctttccc aatatcagat
    13141 ttctatggac ctaaactagt aaaggtattt aagacaccaa tacctgatat gccagtttta
    13201 gcaagtggag ggattaatat tgaaaaccta catatatggt tagaaaatgg agttgatgta
    13261 tgtggatttg gtggtttatt aactaaaggt tctgttgaag atattgctaa aaatgctaaa
    13321 aagattcgtg aaatcattaa aaatgtaaga aaaatttaac atagggctgt atcatagtat
    13381 cttataatta ctatcataca gccttttaat attattcaaa tttaaaacag tttacatttc
    13441 tattatatta catataattt cagtaatttt atcttaattt ttctatctgg aaaaaaatga
    13501 tttaaaactt aagttatgat ataatttcat taaaagaagg cgatttttag taaatatgag
    13561 aaaataatgt gtttttaggg gaaaagttaa atacagattt aagtgaacaa ggatcgataa
    13621 ttaagatatt aaatggtgaa tagggtatca aaaaaattac taaattaaaa gttttagagg
    13681 gagaatctta gcaagtgtgt gtgaaaattc aatttcaaac aaaaaataat tgtaaatgaa
    13741 atcaatttat acacgttgaa gattcacttt tatcattata ttaaaaagca gagtgaatta
    13801 aaagtgagtc taaagatagg aggataatta aaacaatgaa agaagacatt aggttacatc
    13861 gaatgtgtaa aaaagaacaa gcaacaaaga atgaagttat atcagaggat attctaaaat
    13921 ctatgagtat tggggtcagt aaacattttt tagatgattc atttacaata atatggggaa
    13981 atacatcatt ctatcaatta ttaggatata cagaagaaga atttttagtt aaattttcta
    14041 gtttaaaaga ttattatgaa cagaatcttt atcattttga atctatgaaa aatcattttt
    14101 tagaggctta tcatcatggc gaaaagagta tagaatatca aatttgcatt tcagcaaata
    14161 atggagaccc tgtttgggta ataatgactg gtatattaat tgaatcacaa gataatcaga
    14221 aatcaatggt ctatatattg tattcaaaaa ttaatgagtt aaaaataaag caagaagaac
    14281 tatctgatat ggaaaaagag agtacagaga attttaaatg gatgatgtca gagtatgctg
    14341 ggaatgttta tattagcgat atggatacat atgaattact atacttaaat aagcactcat
    14401 gcaatacttt acaggcatca gcaaatgaac ttattggaag aaaatgttat gaggtacttc
    14461 aaggtagaac ttcgccatgt ccgttttgta caaattcgta tttaaaaaaa gatgacacat
    14521 atgaatggga attttataat aaaaaactga aacgaacatt tataattaaa gaccgtatgt
    14581 tgaattggca agggcgaaga gttcgtattg aattatctta tgatatgtat agtgctgaat
    14641 ataaattagc aaaaaaagat caggaaagag aatcaatttt gaaaactatt ccagcaggaa
    14701 tggttagatt agatgcaaga gattatctta ctatactttg gtgtaatgat atatttttga
    14761 atatgattga atatacaaag gaacagtttg cagaggaact taataatcaa tgtggatatt
    14821 tattttctga agattataaa cgtagtaaga aactggcaca agatttgaag gagtctgggg
    14881 ataatgttgt atttgaagca aaaatatata cacgttcaag ggaagaacgt atatggactg
    14941 ttacattgtg ttatatcagt ggggaagaca gttgggatgg aataccatct ttttatagta
    15001 taggtattga cataacaaag gaaagaaaac agattgagaa attacagcat atatcagaga
    15061 aagatgcatt aacaggaatt tataatcgtg ctgagactga aaggcagatt aagaaatatt
    15121 ttgaaaaaaa tttaaatgtt atgggtgcac tgtttatgat tgatacagat aattttaaac
    15181 agattaatga tacagaaggg catatgattg gagatatagt acttactgag atggcatcag
    15241 gtatgaaaaa aataatgcgt gacagtgatg ttgttggaag aataggtgga gatgagttta
    15301 caatttttat gaagaatatt tcctcagtta aagatgcaga aaagaaagca gaagaactat
    15361 tgtatatgtt ccgtcatcta tttcagaaag aaaaaagttt tctaaaagta acttgtagca
    15421 taggaatagc tatatatcca aaggatggta ctacattcaa ggaaatatat gcaagagctg
    15481 ataaagccct gtatcaagca aaaaatatgg gaaaaaataa ttatgtaatc tacaaccaag
    15541 attattttaa agaattagaa gaatttgatt gttcttctct tggtacagtt attgattcag
    15601 aaaaacaata cgctgaatat cctgataatc ttacacgtta tatattccgt atgctttatc
    15661 agactgatga tatagaccag gcgattaata tgatacttga ggttgttggg aaacagtttg
    15721 atgtaagtcg tgtttatatt tttgaaaata cagaagatgg tagatatact tctaatactt
    15781 atgaatggtg taatgaagga atctcttctc aaatggctta tttgcaaaac agcaattatc
    15841 aagactatga ggattatgaa aagctttttg aggatgaatc agttttttat tgtcgagata
    15901 tccatacatt aagtcctaaa ctagaagaat tgttttctag tcaaggtatc cactctacac
    15961 ttcaatgtgc atataaagaa gatagagttt tcagtggatt tgttggattt gatgaatgca
    16021 caggactacg tctatggtca caagaggaaa taagtacatt atcattgatt tctcaaatta
    16081 tatcaatgtt tttacaacgt aaaaaaaata ataaacttaa tcaacagatt caacaatacc
    16141 agaatatttt agataatatt gaccagtgta tttgtgtgat taataaagaa aataattttt
    16201 tattatatgt aaataaaaaa tttaaaaata catattctaa attccaaatt ggacaacctt
    16261 attatcttga ttctatagat attcataaga tatccattat atggaataaa aaagaagcat
    16321 acctatgtac tagcacgatt taatggtaaa gtaaagttta tattggactt aaattaaatt
    16381 tcgttttaaa taagatatta ttttagttga ttactgaaaa gactagaatc tatattaatg
    16441 aattatacta attttatacc actattaaaa tattgtttgc taaaaataaa aaatagcctt
    16501 aaaacagttg attataaaaa attaactgct ttaaggctat ttttatcttt gtgacgaaat
    16561 agacacttgc catttagaat gtgacaatat agtagttgtt tagaaaaggc acaaagcatt
    16621 atactttaac taaagtaaaa tgtattttgc ttttagaata aaaacaatta gga
//
LOCUS       DAEORZ010000091        16504 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEORZ010000091 DAEORZ010000000
VERSION     DAEORZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.091"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(20..1411)
                     /locus_tag="KRH39_002549"
     CDS             complement(20..1411)
                     /locus_tag="KRH39_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG8512442.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(1422..2642)
                     /locus_tag="KRH39_002550"
     CDS             complement(1422..2642)
                     /locus_tag="KRH39_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBG8512443.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIKN
                     NKDNNQKYMASAAK"
     gene            complement(2656..3444)
                     /locus_tag="KRH39_002551"
     CDS             complement(2656..3444)
                     /locus_tag="KRH39_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG8512444.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEEKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3461..4735)
                     /gene="larA"
                     /locus_tag="KRH39_002552"
     CDS             complement(3461..4735)
                     /gene="larA"
                     /locus_tag="KRH39_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBG8512445.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(4853..5566)
                     /locus_tag="KRH39_002553"
     CDS             complement(4853..5566)
                     /locus_tag="KRH39_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG8512446.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5691..6950
                     /locus_tag="KRH39_002554"
     CDS             5691..6950
                     /locus_tag="KRH39_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBG8512447.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            7230..7796
                     /locus_tag="KRH39_002555"
     CDS             7230..7796
                     /locus_tag="KRH39_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG8512448.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(7997..9202)
                     /locus_tag="KRH39_002556"
     CDS             complement(7997..9202)
                     /locus_tag="KRH39_002556"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG8512449.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     YLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9305..9411)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9503..9871)
                     /locus_tag="KRH39_002557"
     CDS             complement(9503..9871)
                     /locus_tag="KRH39_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBG8512450.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(9923..11200)
                     /gene="obgE"
                     /locus_tag="KRH39_002558"
     CDS             complement(9923..11200)
                     /gene="obgE"
                     /locus_tag="KRH39_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBG8512451.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11311..11601)
                     /gene="rpmA"
                     /locus_tag="KRH39_002559"
     CDS             complement(11311..11601)
                     /gene="rpmA"
                     /locus_tag="KRH39_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBG8512452.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11615..11932)
                     /locus_tag="KRH39_002560"
     CDS             complement(11615..11932)
                     /locus_tag="KRH39_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBG8512453.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(11947..12258)
                     /gene="rplU"
                     /locus_tag="KRH39_002561"
     CDS             complement(11947..12258)
                     /gene="rplU"
                     /locus_tag="KRH39_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBG8512454.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12359..13780)
                     /locus_tag="KRH39_002562"
     CDS             complement(12359..13780)
                     /locus_tag="KRH39_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBG8512455.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGNTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13798..14499)
                     /locus_tag="KRH39_002563"
     CDS             complement(13798..14499)
                     /locus_tag="KRH39_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBG8512456.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTSM"
     gene            complement(14501..16345)
                     /locus_tag="KRH39_002564"
     CDS             complement(14501..16345)
                     /locus_tag="KRH39_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBG8512457.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
BASE COUNT         5101 a         3095 c         1753 g         6555 t
ORIGIN      
        1 tattattaaa ttaatatttc taatatattt tccctggatt taaaatattt ttagggtcaa
       61 aagctaactt tatattttta attaacatca tataagcatc tgaatttgat tcatgtaagt
      121 actctttttt agcatatcct atgccatgtt caccagaaac ttgaccacta agttctctag
      181 attttttata catatagtca aaagtttctt ttaatcttat tttccatgta ttatcatcca
      241 taccatcttt taaaatatat atgtgtagat ttccatctcc tgcatgacca aaacttttta
      301 ttcttatttt taatttgtct tgaagctcgt gagtatatct tataaactct gctatcttat
      361 ctcttggaac aacgacatca cattcatcca tttgtgtcgt agatgccttt atagcttcta
      421 aaaatgcacc tctggctgac catatagaat cgtttctttc ttgtgtatct gatataaata
      481 catctaatgc accattttct aaacataaat tagctacttt ttcatattct ttttctatat
      541 cttcagtgct atttccatca aatgtaagta aaagataagc atctgatgta ttatctggaa
      601 actttttacc taaaaattct tctgctgcca atattacatc tctttccata aattctatag
      661 ccgttggtat tgattttgat tttattattt taggaacagt ttctattgcc attgataaat
      721 ctggaaatgg tataagcaaa cttatacttt tctttggtaa aggaagcaat tttaatatag
      781 ctttagtaac tattcctaaa gtaccttctg agcccactaa taaatccttt atactatatc
      841 cagaactatt tttaaccacc tttccaccta catttataat ttctccattt ggtagtacaa
      901 cttcaagtcc tcttacataa tcccttgtaa cgccatattt tactgctctc ataccacctg
      961 cattagtgtt tatattacct gcaattgtag cacttttttc acctgggtca ggtgggtaaa
     1021 ataaatctct atcttgaaca tattgaccta ttgtcatcaa taaaacacct ggttctactg
     1081 tcaatgttaa attttcataa tcaacttcaa gtattttatt cattttacat agattaatca
     1141 ttattccacc atttatggca acagcagcac ctacaagacc agtaccttga cctcttggtg
     1201 tgactggtat attattttta tatgcgtatt tcattatttt agatacttgt tcagtttcaa
     1261 gtacatttac taaaacttca gggtacttct caatccctcc aagttcatca tgtgaaaaat
     1321 cttcatttat atcacttcca actaatacat tttcttctcc acaagtatct attaaaaaat
     1381 ctatatcttt tttatcgatt aatttataca tttctatccc cctatttggc tgctgatgcc
     1441 atatattttt gattattatc tttgttattt tttatttttt caatcaattt aggaataata
     1501 tcataaatat cacctattaa agctaaatgt gctacatcaa gtataggcgc tttatcatct
     1561 ttatttattg ctactatata gtcagcaccc tgcatccctg caacaaattg aactgctcca
     1621 gatacaccac aagttattat caatttaggt tttacagttc taccactaag acctatttgt
     1681 tttttagcat ctatccaacc tgcttctatt acaggtcttg ttcctgccac ttgagcgttt
     1741 aacttatcag ctaatttata tatcatctcc atatcttctt gtttttttat tgctctactt
     1801 gccacaacta tcacttcagc ttcttccaga ccaacttctt tatttttttc ttttacactc
     1861 agtaccttaa ttttagattt tagttctttt ttttctactt tacataaagt tatctttcct
     1921 gtagtattct ctattttttc tggtgctgaa aatattttat atcttacagt ggcaaattgt
     1981 ggtctattat taggtgtgtt tatatgagcc attatatttc caccaaatgc tggtcttatt
     2041 tggtctaaat cagtatttga ttgaatatca agtattgtac aatctgctgt aagacctgtt
     2101 ctaaatctag cggcaagtct tggagctaat gacctaccta atgtagttcc accaactaat
     2161 actatcgttg gttttatttt atctataaag ttttctattg cttttgaata tggttctatc
     2221 ctaaaatctt taaattcatc ttcatcatat acaaatactt catccacccc ataagaaatc
     2281 aaattactgc acttatcttt tatgtctttt cctacaaata cacaatacac aggttgtttt
     2341 atttttgatg ccatctcacg agctttacct ataagctcgt acgttacagg atgtatattt
     2401 ccattatgat gttcaacata tactgcaatt cccctccatt catctttatt aatttgtact
     2461 ttactactct ctataaattc aaatgcgcct tttggtccac ttttgacaca caacttgcac
     2521 atcttacagc cagcacttat ttctactata cctgactttt cttctatagc cccaaaagga
     2581 catatttcta aaattctctt taagtcagtt attttatctt gatttacaac tatctttgac
     2641 atttattact cccccttaca ccaactttaa ttcctctaat ttatttacaa gtgcaaaact
     2701 tagttcatct gcatttcctc ttacaatatt tttatctgta tttggctttg gaggaaatat
     2761 tcttacaact tgagtaggag aaccgttaag tccatagtta ttttcatctt tatcttcaaa
     2821 gtcatctaag ctatatacag gtatttctct atctttagta ttaagtttta atttaaatga
     2881 aggtagtctt ggttgaaaaa tgtctttttc tacagttatt aaacatggat attcaatctc
     2941 acatacttgt aaatcatttg gcatatctat ttcaacaact atactttcat ctttaacttc
     3001 aattaatttt gtaacgttag taacatgagg tatatctaaa aattcagcta cttcaggtcc
     3061 cacctgtgct gtatcaccat cagtagtttg ttttccacaa attattaggt ctacctttcc
     3121 catcttctta ataccttgtg atatggtata ggaagtagcc aatacatctg ctcctccaaa
     3181 tctcctatca cttattaatg ctccttcatc agctcccatc ataaaacttt cttcaattac
     3241 cttctttgcc tgaggaggac ccatgctaag tacttttaga ttcgctttct tctcttcctt
     3301 tattctaaga gctgtttcta aagcatatag gtcataggga ttcatttttg aatctactcc
     3361 atctcttttt agtactcctg tagtttcatc aacttctact gaagtcgttc caggaacttg
     3421 tttaatacat gtcaaaattt ccataaagta cactcctttt ttatttaact ataactgata
     3481 ctccatcagg tataactact actttgctct ccttaccaac tatactattc gccatttcta
     3541 aagcttcttt aaagttaaat gcatgtttca tatgcatttt ttttatcatt tcaggattac
     3601 acttatctgt taccattatc acagtatatt tagataatat tctcgctagt atctgaaatt
     3661 cccattggtc tggaatcgta tctactctat cgacacttct aattttatct aaaagttgtt
     3721 ttggagaatc tgcatttgct acattttcat aaaaagattg acctccatga ccatcattac
     3781 atgctgaaat cattattatt actccaccgt ctttacaagt agcttcagct gctgtcattc
     3841 ctttaacaga ttgataaata ttttggtcta gtggatatcc accatttgta gttacaacaa
     3901 tatcagattt tatagaattt acttttgata actctgttac aaatctacat cctttagtat
     3961 gtgctttttc gacatcacca gcaaaggaag caattatttt tttatcttta tctattacaa
     4021 cattcaatat gaatgctaat tttgcaactt tagctgcata taccatatcc tcatgaatag
     4081 gattattatt caattttcca gttcttgaat tatcactatc tataaagtca ctacagtgat
     4141 tgtacattat agtctttgca gatgcgattc ctggaagtac gctttttctg ccacctgaaa
     4201 aacctgcaaa gaaatgaggt tctataaaac cttctgctat caaaagttct gcctcatatg
     4261 ctagtttgtt tatatataaa tctccacctg aaggcaatat ccctacttta cacatagatt
     4321 tatcatcagt agatatatgc ataactatat cttcattatc tactatttct tctcccattt
     4381 tataaattag ctcttccctt gtacttggcc tatgaaatcc agttgccact ataatcttca
     4441 catcaatttc tggattaaca ttcctaatcc ttcttaatat aattggcata gttattctac
     4501 ttggtacagg tcttgtatgg tcacttgtaa ttatgactac attctttttc cccttaacca
     4561 gttcttccag agaattagag cctataggat tattcataga ttcttcaact atctcttctt
     4621 gagtcttatg ttctatataa ttatccgatt ttgactccaa aacaccgaga aaacgttcat
     4681 caggtatttc taaaatcatt ccttgttttg aatacggtac ttttaacttt accattttta
     4741 atctcccatt ataataaact ataaaattgg tatgtggtca taccagtttt acatttatat
     4801 gatagtacca ttatctacca tttgcaagaa taatttattt tataacaaac ttttaatttt
     4861 cattttttct ataattttta cttataaaat ccatatgttc ctttgctttc tcacatgcaa
     4921 gttctacatc attttctaca actgcattgt atatagcttc atgctgttta agcaatatat
     4981 tttcattaaa aggttctgcg attatttttt ctcttgcatc ttctataaat ccatcaaata
     5041 attttgatgc tgtcataaaa agactttgaa ttaaataatt tttacttaat gttgctagtt
     5101 tactgtgaaa tttttggtct aataacacca gtactcgatt attatctttt ttattagttt
     5161 ctaccctcat ttcatctatt atatgtttca attctataac ttcagaagaa gttattctct
     5221 ttgaagcaag ttttgcaatc tcaatttcta ggattattct aagctctaat atatcttcaa
     5281 gtgtaccatt attaagttta aaaatcatag ataaaggttc tattaaagtt ttttctatat
     5341 ttgtacatat aaaatttcct tcaccctgtc tgctttctat cactcccata gtctctaata
     5401 ccctaatagc ttcccttatg gaagttctac tcacattcat ttgttcagac agttctcttt
     5461 ctgatagtaa tttatccccc ttttttaact ctccatttag tattttatat tgtatttgct
     5521 caattacttg ttcataaact tttttactag ctatactatt aaacattttt tcctccaata
     5581 tgataatcgt tgttgtaata tatatctatt atatatctta aaccattaca ttcatatatt
     5641 tttattactt ttaatataat atcttataac tcaaatttac aggtgatatt atgaaaaaaa
     5701 ataaaattat tgcttttttt gctccatcta tcatagttgg agttctagtt ttagggatta
     5761 tcctataccc aaatgaagct ataaaatctg caagagaagg cttttctata tggtctaatg
     5821 ttttagttcc atcactacta ccatttataa ttggagcaaa cttaattgtt gatttaaaaa
     5881 tagtggatat tatagggttt atcatcaatc ccataactag atttgtattt aatgtttcag
     5941 gaaagagcgc tttagctttt gctatatcta cagtatctgg ttatccagta ggagtaagtt
     6001 tagcttctga atttaggagt aatggacaaa tttctaagta tgaagcacaa agacttgtgt
     6061 ctttttgttc tacatcagga cctttattca taataggttc ggttggtaca ggtatgttcc
     6121 aaaattcaaa cttaggttat ttaatgattc tctgccatta tctaggaact atattggttg
     6181 gtcttttctt tagaaattat ggtaatgaaa atcttccaaa gacaaaattg gatttaaaaa
     6241 caaatataaa aaatgtaatc aacattagaa attcaagtgg gaatggtttt tttgttttat
     6301 ttggaaatgc tgtatttaat ggagtaaaca cattgcttac agttggtgga tttgtaattg
     6361 ttttttcagt cgtatttaaa atactatctc tttttaatgt aatctcatta atagcatcag
     6421 ttatatattt gcctctttca tttttaggtg ttagtaaaga actctgtcaa gcatttgtaa
     6481 gtggtctatt tgaaattact ataggatgca ataaagtatc tagtataaca agttctccag
     6541 aaattttaag agcatcttta gctagttttt tgataggatt tagtggatta tccatcctag
     6601 ctcaatgttg tacatttcta tcaaaaacgg acatagattt aaaattatac atactaagta
     6661 aatttgcgca tggagtttta gctgctatct ttatatttat actttatcca attgcaaact
     6721 ctgctgtgtt ggtttctagc tttgctgata catacaacgt tatatataat aatttaatct
     6781 ggttttatta tcttagctat tatgatacaa ttcttcaaat tgtagtaata atttatctaa
     6841 tctcagccgt atttattgca aaaaaacata tgaaaacttt atctacaaat gggaaactaa
     6901 tgcgatataa aaatactttt ttcactaaga aattttcacg aaaaaaataa aactaaaaat
     6961 aaaactaata aaacttaact aaatacttcc atttttttat tatatttgtc ataattttaa
     7021 taaccttttt aattagttta acagcatagt tatttcatca caatttaata attttattag
     7081 aaatattttt caaaaaattc tccttaatca catatatcac acttgtataa gtttactggg
     7141 aaatttttcc attatataat atttcattta tattatataa taaataccga cctaaactta
     7201 ccaaatcaaa ctaagaggag tggttgaaaa tggcaacaag acctatagaa atagacgaat
     7261 ataaaaagat aatggaacta ttacatactg gctttactta tagtgaaaat ggcgttgaaa
     7321 aaagatttag aaaaaatcct aaagttgcat tggcactaat gttagaagct aatttaggtc
     7381 taagaatatc agatatttta agattaaaga taggtaattt taaaggtaat atgttggaaa
     7441 taaatgaaaa aaagacgggc aaattacagt atagaccagt taataaaaat ataattgagt
     7501 ccataaatga acatgcaaga aagtataatc ttaaatcaaa tgactactta gtaaatataa
     7561 agacaaaggc tattcaaaaa cagttgagaa taatttgtaa atacttaaat ctgtataata
     7621 taagtacaca tagctttaga aagctctatg ctactactca atttgaaaag agtaacaata
     7681 atttagagtt ggttaaagaa cttttaaatc acagctctgt agccactaca cagcgatata
     7741 taagggtaac tcagcaagct attaatgaag ctagtgaaaa tttctttatt ggttaattat
     7801 aacgtataca tttttctatt atcttattaa ttcaccagat attaaaaaag agtgtctcaa
     7861 aatgaacttt ttagttcata agacactctt ttttaataaa atcaaatata tttttattat
     7921 ttcaacactg gaaaaaaggc ttatctctct attttgagac accctattaa attttcataa
     7981 ttcttgcata aacatattaa tctatcttca ataatttttt agctgattca ctgtaattat
     8041 ttctttttac aatttccaaa actctatcaa tctcatgttc tgactttgca cttacaaaaa
     8101 tcgccaattc ttcatcatct ataatatcat aactatctac atatttatct aaacattcta
     8161 aaattccatc tttattatta tacccaccaa atctaactac tgcactacac tcatcttcta
     8221 taagtccact taaatccaat ttcccttttg aaaaagactt tatatttgaa cttctattat
     8281 ctattatatc tataatatct gcataaacag cacttcctgt tgcacctctt ccagctccct
     8341 tacctacaaa agaaagttca cctactgaat cacctactaa aataactacg ttgaactcat
     8401 tatctatctt tgaaagcata ttattattgt ccactaatgc tggtctaaca aatccactta
     8461 cttctcctga ttcatactta cttctagcaa ccaattttat cttacaattt aatttttttg
     8521 catattctat atctttttca tctattgaag ttattccttc taataataaa tctttccaat
     8581 atactatttt tccataagat aaagtagaca gtatagataa ctttctagca ctatcatatc
     8641 ctaaaacatc tgcatctgga ttaacttctg caaatcccaa ctcttgtgcc tgttttaaag
     8701 cgacatcata tggtaaattt tcatcataca tctttgataa tataaaatta caagtcccat
     8761 ttaaaatagc gtttatgctc tttatattgt tgccttccag tgattctatc aatggtttta
     8821 aaactggaat accacctgcc acagatgctt caaacttgat gctaacatta tttaagttag
     8881 ctaattctat aaggtaacta cctttttcag ccagtaaatc tttattagct gtcactacat
     8941 gtattttttt atttaacgct cttgttacat aatcaaaagc tggatttaat cctccaatta
     9001 cttcaactag tatatcaata tccttgtcaa atatatcttc tatatcagta gttatcttat
     9061 caaaatactt cttatacttg tgtttttctt tatttttaac taaaattcca acaacttcta
     9121 tatctctttt tgaagtatta ttttctatta aatcaattaa tccacttcct actgttccat
     9181 accctaatat tgctatcttc actgtaacat acccccatca ttttgattat attaaattat
     9241 ttatatatat aaatatacca ataaaaaaag cttgagtata aactcaagcc atatgacggg
     9301 tttatactca tctttcagca taaatgctgc aggatttagc accaatgtat aattaaatat
     9361 actggttgcc gggcttcaca gggccagtcc ctctgccact cttgataaga ttgctaattt
     9421 aaatttatgt ttaatagtat aatacaattt cttccaatcg tcactatttt tttaacttat
     9481 ttccctaaaa ggatataaac tattatctaa cagctctttt agttggttta cccttttttg
     9541 taacattatt atttttagtc tttgctttcg cctgttcatg tcctggaaaa agtattttag
     9601 gctcttcaat attcctcttg taaatagtaa atttaaaacc aatagcctgc acaaattcag
     9661 ctcttaaagc ttcacatact gcattagcag tttctttagt atcaagacct gcattttcta
     9721 atatagtaac ttttactaat tctctagttc ttaacatacc atctaattgc tctaagaaac
     9781 tctctgtaac accttcttta ccaatttgtg tagtaggctt taaagtattt gccaaagatc
     9841 ttaaatacgc cctttgtttt ccttttaaca tattctcacc tctataattc tatacttaaa
     9901 ttatctataa tctatattta aattaattgt aaaactcaaa ttctaattca taaattttaa
     9961 ccacatctcc atcttctatt cccatttctc taagcctatc aaaaacacct tgactctcca
    10021 tagctttttg gaaatattga agggattcca tatcatcaaa atttacagaa tacattattc
    10081 ttctaagtgc tttacctgta actacataca caccatcttc aatatctata cttaactctt
    10141 cttcagtacc aatatcaagt tctggtctat acatttcttc ttctgaaact agctccacat
    10201 cttctacttc ttttagcatt ttagaaacat aggctattac ttcatctatg ccttgacgag
    10261 ttgctgctga cattttaaat actgtatatc ctctgccttc taattcactc ttaaacttct
    10321 caaacttact ctcatcttct aatatatcaa atttatttgc aactacaact tgtggtctct
    10381 ttgaaagctt ttcattgtac aatttaagct catcatttat cttgtcaaaa tcttcaatag
    10441 ggtctcttcc ctctaatcca gatatatcta caatatgtat caataccttt gttctctcaa
    10501 catgtcttaa gaactcatga cctaatccta tgccttcaga agcaccctct ataattccag
    10561 gtatatctgc aagtacaaaa ctatctccaa acttagtttg aacaacacct agatttggtg
    10621 taagtgttgt aaaatgatag tttgcaattt ttggtttagc tttagttaca actgatagaa
    10681 atgttgattt acctacattt ggaaatccta aaagaccaac atctgctatc attttaagtt
    10741 caagagttat ccatctttct tctccatctg taccagattt tgcaaaagct ggagcttgtc
    10801 ttactgcatt tgcaaagtgt tggttacctt taccacctat accacctttt gcaactatag
    10861 ctgtatctcc ttcttttttt aaatctgcta atacaagacc tgtagcctca tctcttatta
    10921 ctgtacctgc tggtactttt aatactaaat tttctccatt tttaccagct ctttttcctt
    10981 tgcttccgtc gccaccattt tgagcttgat attttttctg gtacttaaag tccattagag
    11041 ttctaagccc caaatcaact tcaaatatga tgcttgctcc acgaccacca tctccaccgt
    11101 ctggaccacc agctggaacg tatttttctc ttctaaatgc taccgaacca tttccaccat
    11161 ttccagcctt aacaaatatt cttgccttat ctataaacaa attcttcacc tacttaatta
    11221 aaaactttta attagcatta ccaaaaatac cattataaat tcatcagtat tataagaaaa
    11281 aggagtgcct caacactcct tttatgtata ttactcagct attgctactg gatatatact
    11341 aacttttttt ctcttcttgt cttttctttc gaatttaaca gtaccatcaa ctaatgcaaa
    11401 taaagtatca tcactacctt ttccaacatt agttcctgga tgtattttag ttcctctttg
    11461 tctaactatt atacttccag cagttactaa ttgaccatcg aatctcttaa cacctaatct
    11521 tttagatata gaatctctac catttttaga agaacctacc ccttttttag atgctaataa
    11581 ttgtaaattc atgtttaaca tatggagtca ccccctactt ttttaaaatt tttatatttt
    11641 ttgggtattc ccgtttaatt tctttaaccg ctaattgaaa gtgatttagt aataattgtg
    11701 cgtcttttac atgtgtatct ttgactatac actctatatg accttcttct cctactactt
    11761 tttcaaattc aatcttttgt aatttgtcta aagaatttat aactgcaata gcattagatg
    11821 taactgcagc acatactata tcatagccta tttcagcaaa atcagcatgt ccctttaaac
    11881 aaaaaccttg caacaagaaa tcatcattat aataatattt aacttttatc ataacttaac
    11941 caataactaa gcgtttattt tttctacaac tatcttagtg tatgattgac gatgtccttg
    12001 ttttcttctg taatcttttt ttgctttata cttgaaaact ataactttct tagctttacc
    12061 ttgttcaaca actttagctt gaacagatgc tccttctact actggagaac ctaattttaa
    12121 ttctccatct ttagagcaag ctaatacttc gtttaaagta acaacatcac ctgcgttagc
    12181 ttctaatttt tcaacgaata atacatcacc ttcagaaact ttatattgct ttccaccagt
    12241 ctttactata gcgtacatta aatacacctc ctcggcctca aactcgccag tacaaggtac
    12301 ttcatttgaa gatttaaacc tttactgtgc ggcattacaa ttattaatta taaaatattt
    12361 acattttatt gtcaactatt ttatcgaaaa taacattggt tttattgtgc tttaatgtac
    12421 tatttgcgtt taaagaaatc tttatattat atttttcacc aattttatca attatatcca
    12481 tataatattc ttttatttgt tcaaaaataa aatcattaaa ttctaccatt atgtttttgt
    12541 aaacagtatg ttcacttatt ctcataattt ctttttcaat actatctagt atataatgta
    12601 cagattttat ggtttgttca ccatcacaac atggacaaga cattaaataa tacttatcaa
    12661 tggattcttt ttctctacgc ctagcaactt ctactaaacc aagttttgtc atacctaata
    12721 cttcagcttt tcttttatct ttattaaatt cttcttctag gatgtgtaaa atctcctttt
    12781 gatgcttctt tttatgcata tcaataaaat cgattattat tatcccagct atgtctctaa
    12841 ttctaagctg tcttacaatt tcttttgcag cttcaagatt agttttataa acggtttcat
    12901 caagtttacc agttccaata aacttacccg tatttacatc tataaccgta agagcttctg
    12961 ttttttcaat tattaaataa cctccactct ttaaccatac ttttttattt aataattttt
    13021 ctattttaga ttctattctg tataaatcaa atacatctct attttcctca agacacagtt
    13081 tatcaacata agtcttatct atacctctta aaatagactt aagttcagag tacttatcat
    13141 aactattggt aattatagat tcaatatcat cattcacatt atcttttaca tatttagttg
    13201 caaaatctaa agacttatat aataatttcg gacctatacc aagtttatat tcttttaaaa
    13261 tgttttcata ctttaatttc aactcttcta tatctttctt tatttcatca tgagtgcaac
    13321 cttgagcctc agtccttatt attaatccta gattttcctt atttactgcc ttagtaattt
    13381 tttttaatct aaatcgttct ttttcatcag taattttatt tgatattgta gttctatcat
    13441 tggaaggtat atagacaata taccttccag aaatactaat ctcagtgttt aattttgcgc
    13501 ctttagttcc tatttcttct ttatttatct gaaccaaaat atcctgacca gattttatag
    13561 tatttccctt ttttaattgt aaataagcaa gtttcttaaa tccaatatct acaaaacaag
    13621 cttctattcc tggtataact ttttttacga ttccacgata tatattggat acagtcttct
    13681 tatttaaatt gtcttcaaca aaaagttctg ttaatctttc atcttcaagt actgctgttt
    13741 tctgggaccc tattagggac tcaataacta tctttttcac aacttcacct ctcgagctta
    13801 catagaagtc actaattccc catcttctaa tacatataaa tctcttctca atatatcaac
    13861 atctaatggg tcaatatcaa gttcaaacat ctctaatatc tttggtataa gtatattagt
    13921 atttaaatta gtttttgagc ctgtagctat agtagcagta agagtaatat gttcatcttc
    13981 taaatttaaa acatcaaagg ttcttatcat aggtcttata tctacttcaa ccatcttacc
    14041 ttttttattc ttttttgtta tgattatttc tttattattc ataaagtcaa ttacttttct
    14101 ttttacaaac tctttcgtca taggtctttt caaatctata ttaaacaaat attctccata
    14161 gtctatcaca gaagaaagag aaggtgtttg tctatcaatt tcttttgctt tgataaaatc
    14221 tataccatct ggaagttgaa ttgatacttt atttaaaaat tcctctactg ataaatcatc
    14281 ttcttctatt tcaatatcta catactcacc ttgactttct gtccctaaag ccaaagcatt
    14341 gccataactc attttagggt gaggattaaa tccttgagaa tgtgataagt ttatttcagc
    14401 tcttctaaac gctctttgta acaatctctg aaggtcaaga tgtgatatat atatcatgtc
    14461 gccttccttt ttaaatttta ctcttattat cttactcata ttagcaaagc ccccttccta
    14521 tatcatgtat atttattcca caaccattgc agttatgtct acaatcagaa gtgatttttt
    14581 cttttttagc attttcgtct tctctaatta agaatttttt actaatacct acatctatat
    14641 ggtcccaagg aaatacctct tcatagtttc tatttctatg tgcataaaaa tctatagaaa
    14701 gattattctt ttccatagct tctttccata tatctaaatt aaaatgttct gcccatccat
    14761 caaatttagc acctagtcta aatgcatcat atataacttt tcctattctt ctatcgcctc
    14821 tagctactac tgcttccatc aaactagtct ttgaatcatg ataattgtat tttatattgt
    14881 tatttcttaa tttatttact aaatgtctct gcttattaat aacttcttct gtagtatctt
    14941 gaccatgcca ttggaaaggt gtaaatggct ttggtacaaa tgtagaagta ctcactgtaa
    15001 cactaaaact tctctttagc tttcctccat ttacttttct ataaatatca ataactttat
    15061 aagctaattt agctatacca tctaaatctt cataagtctc agttggtaaa ccaatcataa
    15121 aatacaactt aacactattc caccccatct caaaagctct ttctgttgca ttttccaaat
    15181 cttcttctgt aacaccttta tttataacat ctctaagtct ttgagttcct gcttctggag
    15241 caaaagtaag tccagatttt cttacttgtt gtattttatc agcaatttcc attgaaaaat
    15301 tatctaatct aagggatgga agcgaaatcc caatattatt agatgcatat tcatctacca
    15361 aataatcagt caactctgat aacttactat aatcactcgt actaagtgat gataatgata
    15421 tttcatcata tccagtattc ttaactaatt tatctaatat ttctttaagt cttttaacac
    15481 tcttttctct tattggcctg tatatcatac ctgcttgaca gaatctacat cctctagtac
    15541 atcctctaaa taattctagt actattctat catgaacagt atctatataa ggtactatta
    15601 gtttttctgg ataatctaca gtctcaacat cttttattat ccttttagta ggatttttag
    15661 taatcccttc tctattagga gttacacttt gaactgtacc atcttcattg tacttgacat
    15721 cataaaaact tggtatgtat actccttcta tgctagatat tttatataaa aaatcttctc
    15781 tagtcgtttt atttttcttc cattctttat actcatttac tacttctaaa ttaacctctt
    15841 caccttcacc taatattact acatctacaa aatctgctaa tggttcggaa ttataagcac
    15901 aaggaccacc aaccattata aatgggtctt ccaaagttct ttcttctttt aatattggta
    15961 tattggctaa atctaatata tttaagatat tagtgtatga aagttcatat tgaagtgtaa
    16021 aagtgacaaa atcaaagttt gttataggct ctcttgactc taatgcaaat aatggtatac
    16081 ttttttctct cattatattt tccatatcaa ctgcaggaga atacactctc tcacaaaata
    16141 catcttcatc cttatttatt acatcatata atatatgact ccctaagtga ctcataccaa
    16201 cttcatataa atctggaaag caatgtgcat atcttatcaa ttcactatta ctagtatctt
    16261 tatgaattga atttatttca tttccaagat atctagcagg cttttctact ttttttaaga
    16321 tttttttcaa gtctacttta ttcatttatt aaaattcctc caaacaagct ctttattttt
    16381 attcttattt ataagtaata ttatacacga taaaatattt tctatcaaaa atataactaa
    16441 aatattgaat ttaaaataca tattattcac aaaatagaat agctaaaaat atttaagaat
    16501 attt
//
LOCUS       DAEORZ010000092        16431 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEORZ010000092 DAEORZ010000000
VERSION     DAEORZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16431
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.092"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            188..1249
                     /locus_tag="KRH39_002565"
     CDS             188..1249
                     /locus_tag="KRH39_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBG8512458.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLTVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            1340..2953
                     /locus_tag="KRH39_002566"
     CDS             1340..2953
                     /locus_tag="KRH39_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG8512459.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKELRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            3228..5213
                     /locus_tag="KRH39_002567"
     CDS             3228..5213
                     /locus_tag="KRH39_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG8512460.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            5218..5652
                     /locus_tag="KRH39_002568"
     CDS             5218..5652
                     /locus_tag="KRH39_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBG8512461.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKNLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            5681..6241
                     /gene="srlA"
                     /locus_tag="KRH39_002569"
     CDS             5681..6241
                     /gene="srlA"
                     /locus_tag="KRH39_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG8512462.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            6305..6673
                     /locus_tag="KRH39_002570"
     CDS             6305..6673
                     /locus_tag="KRH39_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBG8512463.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            6698..7264
                     /locus_tag="KRH39_002571"
     CDS             6698..7264
                     /locus_tag="KRH39_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG8512464.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            7305..7670
                     /locus_tag="KRH39_002572"
     CDS             7305..7670
                     /locus_tag="KRH39_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG8512465.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            7771..8544
                     /gene="srlD"
                     /locus_tag="KRH39_002573"
     CDS             7771..8544
                     /gene="srlD"
                     /locus_tag="KRH39_002573"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBG8512466.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            8893..11121
                     /locus_tag="KRH39_002574"
     CDS             8893..11121
                     /locus_tag="KRH39_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG8512467.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKIDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDKLDTTSNKVNYISDELD
                     TTSNKVNCVSDELDMTSNKVNYVSDELDITSNKTDYISNELYNLSNKSIKGNIPIKMA
                     VITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTATLCDMFIPCTNTLESEDIIY
                     SSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVEKRTYLEKVIEPLKR
                     FDKNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESIKNLGISPTPVYISNKY
                     KEIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINRRMAKKVGIEDKDIVILRSK
                     KAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFGGQVTYNESMVEIIR
                     QN"
     gene            11209..11544
                     /gene="spoIIAA"
                     /locus_tag="KRH39_002575"
     CDS             11209..11544
                     /gene="spoIIAA"
                     /locus_tag="KRH39_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBG8512468.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            11558..11986
                     /locus_tag="KRH39_002576"
     CDS             11558..11986
                     /locus_tag="KRH39_002576"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBG8512469.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            12009..12773
                     /gene="sigF"
                     /locus_tag="KRH39_002577"
     CDS             12009..12773
                     /gene="sigF"
                     /locus_tag="KRH39_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBG8512470.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            13053..13490
                     /gene="spoVAC"
                     /locus_tag="KRH39_002578"
     CDS             13053..13490
                     /gene="spoVAC"
                     /locus_tag="KRH39_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBG8512471.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            13657..14670
                     /gene="spoVAD"
                     /locus_tag="KRH39_002579"
     CDS             13657..14670
                     /gene="spoVAD"
                     /locus_tag="KRH39_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBG8512472.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            14684..15046
                     /gene="spoVAE"
                     /locus_tag="KRH39_002580"
     CDS             14684..15046
                     /gene="spoVAE"
                     /locus_tag="KRH39_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBG8512473.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            15229..16245
                     /locus_tag="KRH39_002581"
     CDS             15229..16245
                     /locus_tag="KRH39_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG8512474.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT         6576 a         1670 c         3035 g         5150 t
ORIGIN      
        1 aataatagca tattattcgt atcctgtata cataaaaaat aaaaaattgt cgctttaaag
       61 tataaaacag attatatgtt aataatgtgg tgaaattata cccatatata aataaaataa
      121 aacaaataaa tagctaatta aggagaaaat attttttatt ttttgtttag gataaataaa
      181 ttttattatg gaaaaaagaa aaagcataca tcttcacaaa ctgaatattt atttaatagg
      241 gggaggtgat agattgttta tcacagtggt attattttta gtaggtttcc tattaattac
      301 taaaggcgca gatatcttta taaattgtac tgtagagata ggtaaaaaaa cgaatatatc
      361 agaaattata cttggagcta ctatagttag ttttgcaaca acattacctg aatttacagt
      421 gtctctactt gcttctattg atggtcatac tactatgagt ttaggaaatg cagttggttc
      481 tataatatgt aatacagggt tagcattagg gttggttgtt tttataagtc catttaatgt
      541 agataaaaaa atgttttttt ctaagtcttt actattaata gtatccgtta tagttttaat
      601 tttattgagt ttagatggag ttataacaag aggtgattca ttattactta taataatatt
      661 aatcttttat atggttaata attatagaag tgtcgttgga aaatctgata ctaaaaatag
      721 aaatattaaa aataacacta ttaaagacat atctactaaa aaagggaaaa attatagggg
      781 tttttccgta ttagaaattg taaaaatact attacttttt gctacaggat tgataatgat
      841 gataataggt tcacaaatcc ttatagagag tggagtaata atagctagtt ttttaaatat
      901 acctcaaggt atagtaagtt taactataat agcacttgga acttctctgc cagagatagt
      961 ttcgtcgatt actgctataa gaaaaaatca tcatgaaata tcagtaggaa atattttggg
     1021 tgcaaacata ttgaatatag tatcagtaat agctgtatca gcaataccaa ataacatacc
     1081 aatactatct caaaatagac aattggatat accattcatg atattattat tattaacagt
     1141 aataatacca acacttaaat ctaataaact tagtagaata caaggtatat tgatgttatt
     1201 cacatatttt ctgtacatat caattttgta tttcatgtat attatttaac tatactgttt
     1261 aaaatattaa ttgcagaata tcctaaaaaa gtatataatg gataggttaa gattaattga
     1321 taacggagag gaataatata tgaatataac aaagtttgaa gatttaccaa taagtgaggg
     1381 aataaaaaaa gctatagctg aaatggggtt tgaagaacca tctcctatac aagcacagtc
     1441 tatacctgcg attttgtcag gtaaagacgt tataggacaa gctcaaactg gtacgggaaa
     1501 aacagctgct tttagtatcc ctatactaga aacaatagac ccaaataata gaagtttaca
     1561 agctgtagtg ctttgtccaa caagagaact ggcaatacaa gtatcaacag aaataagaaa
     1621 gttagctaag tattcgcacg gaataaagac attacctata tatggtggtc agccaataga
     1681 tagacaaata aaatctctaa agagtggtgt gcaagttgta ataggtacac caggacgtac
     1741 aatagaccat atcaatcgta aaacattaaa gatggataat gtaaaaatga ttatattaga
     1801 tgaagcagat gaaatgctag atatgggatt tagagaagat atagaaatga ttttaagtaa
     1861 gattccagaa gaaagacaaa caactttctt ttcagctact atgccaagag gaattttgga
     1921 attaactaag agatatcaaa aagacccaga gcatataaaa gttgttagaa aagaacttac
     1981 agtttcaaat acaaagcaat actatataga aactaggtca tcaaataaat tagaagtatt
     2041 atgtagatta gtagatgtat atgaccctaa actatctgtt gttttctgta atactaagag
     2101 aaaagcagac gaattggtag gagatttaca agcaagagga tattttgcag atgcattaca
     2161 tggtgactta aaacagactc aaagagatat agtaatggat aagtttagaa atggtacaat
     2221 agatatactt gttgctactg atgttgcagc tagaggtata gatgttgatg atgttgaatg
     2281 tgtattcaac tatgacttac cacaagatga agaatattac gttcatagaa ttggtagaac
     2341 tggtcgtgct ggtagagaag gtatgtcatt tacttttgta tttggaaaag aactgagaaa
     2401 gatgaaagat atagaaagat atactaaatc taaactaata aagcataata taccaacaat
     2461 aactgatgtt gaagagaaaa aagtaggtac tttctttgca caagttaaac aaactataga
     2521 agaaggtcac ttaactaaac aattacaatg gttagaaggt ttctgtaatg atgaagatta
     2581 tgcaatggtt gatatagctg ctgcacttgt aaaactatct ctaggtgaag aaatgaaaga
     2641 agaaataata gaagaaaaac ctagaagaga gcgtggagat agaaaaggtg gtactggagc
     2701 taaagatggt atgattagat tatttatcaa tattggtaga aatcaaagag tacaagcaaa
     2761 agatatagtt ggtgccatag ctggagaagt tggaatacca ggtaaagtag taggtactat
     2821 agatatatat gataagtata cttttgttga aatacctaaa aaagatgcaa aaacagtaat
     2881 tgaaaagatg aaagatatta agataaaagg taataagatt aatatagaaa aagcaaataa
     2941 gaagaaaaaa taacgtatag tatacaaaac tataattaga aaatatataa aataatatat
     3001 tggatattta aaaagcctga tgtgttttca aaacagatag tagataatct gtaagagtta
     3061 acagcataag gcttttcttg ttttatatga tgttataatt ttccagattg ttgtgaaaaa
     3121 tgtatattag ttcctttttt tcaaatctga tttgaaaaag agaatattaa ttatctatat
     3181 agtttagatt ataataaaaa atgtaagata acaaagggga gaaaaagatg tcaatgactt
     3241 atatggaccc tagggttttt ttgattttag aaatggtgtt agaagaagac acaaatactc
     3301 tagaacaatt ttcaaatgtt ttgggtgtta gtactaggac tatcagaaac tatataaacc
     3361 agattaatcg agaattaaaa ggtggtattg ctaaaatagt taaaaatgaa aaaggtacat
     3421 atagtataag aattgaggat gaaagtaaat taattgagat tgtaaatttt aatagaggaa
     3481 agctatcaaa ttttataaat ttaaattcac ctgatgaaag aataaattat gttcttgata
     3541 ttttaactat gaccaacaat ccaattacca tagatgattt ggctgaagag atatgtatag
     3601 gaagaacaac attaataaag gatttaaaaa aagcagacag aattttagct gaatatgatt
     3661 tagaattaaa aaggaagcca aatataggta tgattttagt tggtgaagaa atggatataa
     3721 gattattaat ccttgataga ttgtatgaaa attatattga tgtcttggaa aacgttaatc
     3781 agataaattt agttaaagat tatgatattg aatgtcttag agatgaatta aaaaagttat
     3841 ttaaaaaaga agaactctac ataacagaac aggtcttgag agatgttgaa agatatataa
     3901 tcatgtctgt cttaagaaat ctaaatgggt acaaatttga aaagatagat aagagatttg
     3961 aagtgataag atgttcagat gaatatactt taggattaaa gctcaaagct ttattggaaa
     4021 agatatttaa tatagtttta aatgaaaaag aaaccatatt tttgacaatg ccattaatag
     4081 gtagaaaagc tcctagtaca aaattagcac ttagcagtat aaagataaat gatagcgtaa
     4141 aggaagttgt agatgaagta actagttttt tattagagac ctcagggatt gattttaaag
     4201 aagataaaaa gcttatagaa aatttggaat atcacttgta ttttgcactt aatagaatga
     4261 gattcaacat aagggtaaaa aatcctcttt tacaagaaat aaaggaaaga tatacatttc
     4321 catatagtgt tgcaaagata gctgcaatga taattgagga aaagtttgac ttgaaaatat
     4381 gtgaccacga aataggatat atagcattac attttggaag ttattttgaa aaaaattcaa
     4441 gaaaagttct aaatgtaaat agggtagctg ttgtttgtgg aacaggtctt gggactgcac
     4501 aacttattag aataaagata ggaaaaatac ttggagaaag tgtagaagta aatacttttt
     4561 ctgatataga agctaaagct aatttagatt tattaaagta ttatgattta gtatttacaa
     4621 cagtagattt caaaacagac ttagatgttc caatttttag agtaaatgcg ttatttaatg
     4681 aagaaagtat aaagaaagag atagaaaatg caatactttt gaaaaatagt ataaatctta
     4741 agtctataaa aaagattgaa aaaaatatcc cttttatagg taaattaatt gaagaggata
     4801 agttctttat attgaataag agtacgttta tggaaaattt agaagatatg ttagaaaaat
     4861 tagttcataa aggaattatt gatatagagt tcaaaaaaaa ggtattaaaa agagaaaaga
     4921 aatcttcaac agcattagat aattatatag cattgcctca ctcagttaat actaatgggg
     4981 aaaatcttta tctggcattt ggaatattag aaaaaccagt gatttgggat acaaaggaaa
     5041 ttagaataat aatattgatg ataattccag ataagaatgt agatagtaca gacctgataa
     5101 taaaaagtta tgaagaagta cttgaagtag ggagagataa aaagatggta gaagaacttg
     5161 ctaaggcaaa atcatttgat gaattttata aaataattac caagaggagg taataatatg
     5221 gacccagtaa tttttattat agcatcactt attacagtgt atttattaaa tatttttctt
     5281 ggatacttac agcttaaaga ttttaataaa aattatattg aactaaaaag aaaaggaaga
     5341 gtggcaattg gcagaaaaaa aggaagaatt tcatcgggta caattgtgtt gatacttata
     5401 aatgatgatg gtgtgattgt cgagactaga aaaatgcaag gtgttacagt attggcaaga
     5461 gtaaagtcat ttgaaggttt ggtaggaaag aacttagagt atatagaaaa atcagattta
     5521 actgtttaca ataaactact taaaaaagca atactagatg cagtcaaaca gtacataaca
     5581 tttgagaaaa ataaatgtga agatacaaat aatctgagta ctgatgaaga gtgtaggatg
     5641 gaagtagttt aaaaaaatat atttataagg aggagttaat atgacgcaat tttttcaagt
     5701 attagcaagt ggtgcagata gttttatgaa tttatttagg gcaggtgggc aacaatttgt
     5761 aggttttgta acagatatag taccactttt agtagcatta ttagttacaa tgaatgcaat
     5821 tatcaacttt attggtacag atagagtcga gagacttgca aaaaaatgtt ctgcaaatat
     5881 atttactaga tatttgatat taccagtcct tggtacattt gtatttgcaa acccaatgac
     5941 actatcatta ggaagattct tgccagaaaa gtttaaacct agttactttg cagcagcaag
     6001 cttttcatgc catacaatga atggattatt cccacatatc aatccaggtg aactatttat
     6061 atatctagga attgcaaatg gtataacaac tttgggattc tcaacagcag atttagcagt
     6121 tagatatttg ttagtgggta tagttgcaaa ctttattaaa ggtgtagtta ctgactatac
     6181 aacaaaattt gtagagaaac aacaaggaat agcattaaat tcagatataa agattggata
     6241 attactttaa ggtataaata aatttttcaa ataaaaagat agtgtataga ggaggataaa
     6301 ttatatggca tttaacaaag ttaaaatagt aaaaggttct ggaggatggg gtggaccatt
     6361 gataatagaa ccaacagaaa agaagaataa ggtagtatat gtgactgggg gagcaaagcc
     6421 agaaactgca gtaaaaatag ctgaacttac aggatgtgag ttaatagatg gattttcaca
     6481 gggtgttaaa gatgatgaaa tcgcatgtgt aataataaat tgtggtggaa cattgagatg
     6541 tggtatatat cctcaaaaga aaataccgac tataaatata atgaaaacag gaaaaagtgg
     6601 accattagca atgtttataa aagaagatat atatgtttca ggggtaaaac caacaaatgt
     6661 agagcagtta taattgaggt agtaggaggg aagaaatatg gcttatgata ctaaaaagaa
     6721 gataagtgag cagagtcaag gaattgttgc aaaaataggt atagcactag gaaatgtgac
     6781 aagtactctt tttcaagctg gtagggatac tatagataca ttaatacata ctatactgcc
     6841 ttttatggct ttcgttgcag tcctaatagg tataataaat caatcaggta taggaaattg
     6901 ggtagccaat ttacttactc cattaatcgg aaatgttgga ggtttagttg tcttatcaat
     6961 aatatgttca tttccgttgt tgtctccgtt tcttggacca ggagctgtta tagctcagat
     7021 agtgggagta ttggttggta cagaaatagg aaaaggaaat attccaccaa gtttagcatt
     7081 accagcattg tttgcaataa actcacaagc agcttgtgac tttataccag taggtcttgg
     7141 tcttgcagaa gctgaacctg atacagttca agtaggagtt ccgtcagttt tatattctag
     7201 atttttaaca ggtgcaccaa ctgttctatt agcatggtta gcaagctttg ggttatataa
     7261 atagaagaat actttataaa taattagaga gaggttcagt tgatatggaa ttaatataca
     7321 gtactcaaat aaataaaata ggagataatg caaatgaatt tttacaacat aatatgtttg
     7381 taactttcaa agataatgct cctcaagagt tagaaaatta ctgttatata cattctgaaa
     7441 ataatttagt aaaagaaata cttacggatg atgtgctatc aataaataat gttgattaca
     7501 aaattacttc tgttggtgag ttagtaaacc aaaatctttc agaattaggt catataacat
     7561 ttaagtttac aggtgaaaaa gatggagcaa ttggaggaac cttatatctt gaagaaaaag
     7621 aaatagcacc tattggcgta ggtactatac taaaaataat tagaaaataa aatttaaaac
     7681 gaatctaatc atattataag taaattatag ataaaagtca atttaactaa tcaagttaac
     7741 ttttaatgtg ataaaaaagg ggaaatgaaa atgagaaaag tggctttagt cgttggagga
     7801 ggaagaagtt taggtgaata tttatctaaa catttagcta aagtaggtta tgatgttgct
     7861 attgcagatt taaatgaaga caatgcaaaa aaagtagctg aagaaataaa aactgaattt
     7921 ggttgtaaat ctgtaggata tagttgtaat gccatagatg aaagacaggt tataagtact
     7981 gtcaaagcta ttgaaaatga ttttgataga atagatttat tagtgtacaa tgcaggagtg
     8041 gctgtaagta aaaaaataac tgattttgga tacaaagatt ttaaatttat tgtagacgta
     8101 aatttaaatg ggtactttct atttgcaaga gaagtatcta aagtcatgat agcaaaaggt
     8161 attgagggta gaataataca gataaattca aaatcaggta gagtgggaag caagcataat
     8221 tcaggatatt cagcaggtaa atttgctgga gtaggactta ctcaaagttt agcgctagat
     8281 ttggcagaac ataagataac tgtaaatgca ctgatgctcg gtaatttact tgattctgat
     8341 atgtttgaaa gtttaattcc tcaatatgca gtaaaattgg gaatacctga atcagaagtc
     8401 aagcaagttt atatagataa agtaccttta aaaagagggt gtagatttat agatgttgca
     8461 aatatgctta cattttatgc ttcaaaggaa gctgaatact gcactggtca atctataaat
     8521 attactggtg gacaagttat gtaaggtttt tatggagtgt ctcaagatga actctttagt
     8581 tcatgaggca cttttttgta taaaattaaa tatattttca gcatttaaac aatgaaaaag
     8641 aggtttatct ttattttgag atagcctcat ttttgtaatt aaaaaacata gtgtttaagt
     8701 aaacatataa gattgttagt tttttaaaag agaatttttt aataatatta tatttatgaa
     8761 gtatttttaa acttattttg ataaaatata gctatgtttt atatactgta gttttgaaca
     8821 tagaaagttt aataaattat agtatatatg ttaattttta ataaggtaag aaagtagaaa
     8881 gaaggatatt ttatggatat aataaaaaaa ttaagtcatg ggtgtacatt agattgtcat
     8941 gactgttgta aattcaatgt ttatactaaa ggaaataatg tagtaaaaat tgaaggagat
     9001 aaaaatcatc cttatacaaa aggttttata tgcaaaaaag ggatggccca tttagataga
     9061 ttaaatcata aagatagaat aaagactcct atgcttaaaa ttgatggagt atggaaagag
     9121 atttcttttg ataaagctat agaaataatg gcagaaaagc ttacatacta taaagaaaag
     9181 tatacatcaa agtctgtcat gcattatgac caatatggaa gtgggtctgt gctaaaatac
     9241 ataggagata tattttttaa tttttatgga ggtgttagca gacataaggg gggtccctgt
     9301 tggagtgcag ggatgcatgc tcaaaaatat gattttggag tagctaagag tcatgctata
     9361 gaagatatgt taaatagtaa aagtatattt gtttggggaa aaaatccagc atatactaca
     9421 attcatacta tgcaaataat taaaaaagct aaagaaaaag ggataaagat agtagtgatt
     9481 gaccccatat atacaaaaac agctcaaata gcagataagt atgtacaagt aaatcctgga
     9541 acagatggag ctttagctat agctatggca aaaattatag tggaagataa attatatgat
     9601 gaagaatata taaactcgta tgttattggt tttgaagaat ataaaaaata tttatcttca
     9661 ttggaattaa gttttttaat tgatgaatgt ggtgttaaag agaatgatat aagagagtta
     9721 gtggatttat atactaacaa atattcaagt ataaatgttg gatatgggct tcaaaaatat
     9781 aaaaatggtg gaaacacaat aagagcaata gatgctttag gtgctataac tggtcaaata
     9841 ggatttagtg gtggaggggt aaactatgct aataaagttt atccgagtgt tataaattct
     9901 gacccttaca atagccaatc atatggtgaa gatagggagt tttatgtaag taatatcagt
     9961 aaatttatag aagaatcttt aaaaaataca tcaaataaag taaattatgc atcagataaa
    10021 cttgatacaa catcaaataa agtaaattac atatcagatg aacttgatac aacatcaaat
    10081 aaagtaaatt gtgtatcaga tgaacttgat atgacatcaa ataaagtaaa ttatgtatca
    10141 gatgaacttg atataacatc aaataaaaca gattatatat caaatgaatt gtataattta
    10201 tctaataagt caatcaaagg taatattcct ataaagatgg ctgtgataac taaaagtaat
    10261 atgctgaatc aactgccaga tttagtagaa ttggagaggg tgttttcaaa aattgaattt
    10321 aaagtatgtt ttgatatgtt tataacggat acagctactt tatgtgacat gtttataccg
    10381 tgtacaaata cactagagag tgaagatata atttatagct caatgaccaa tccatatata
    10441 acctataatg aaagagctgt aaagccagca cataaattga tggatgaata ttacttcttt
    10501 atggagctag ctaaaaagat gggattaaat gattatcctt ttgtagaaaa gagaacttat
    10561 ttagaaaaag tgattgaacc acttaagcgt tttgacaaaa atctggatat agaaaaactt
    10621 aaaaacaatt actttactat acataaccct gttgcttggg aagataagaa gtttgagact
    10681 ccatctggaa aatacgagct ttattcagaa agtataaaaa acttgggaat aagtccaaca
    10741 cctgtatata taagcaataa atataaagaa atagaagata aaaatatttc ttttagatta
    10801 ttaactaatc atcatgcaga tactttattt agtcagcatt ttatggacaa aaaatctata
    10861 gctcaagcat atataaatcg gagaatggct aaaaaagtag gtattgagga caaagatatt
    10921 gtgatattga ggtcaaaaaa agcaaaaata aatgtacaaa taaatataga tgatggagta
    10981 ggtaattata tagtaaaaat gtatgttggt tggtggaaga aacatggaaa tcctaatagt
    11041 ttaacagaca ctggtatatc agattttggg ggtcaggtaa catataatga aagtatggta
    11101 gaaataatac gacaaaacta gttaaatttc attgaaggaa taaaaatata attatagaat
    11161 tgattaaaag ttactatttt tacaaaacta ttgaaggagg gatttttgat ggtaaattat
    11221 tccttagaac ataagaatct atacatagaa tttatgtgct cagaactaga ccaccatgta
    11281 gccaatgaaa taagggaaga aatagataac ttattaagtg taaatcaagt aaaaaatgtt
    11341 gtatttaatt ttgaaaatat taattttatg gattcttcag gtataggcgt catcataggt
    11401 agatataaaa agatttctaa tgaaggggga cgagtttcag taattaatat aagttcacga
    11461 gttaagaaga tttttgattt atctgggtta aataaaatta ttggcattta tgatacgtat
    11521 gaagaagctc taagttcttt gtagggagga gtatgtgatg aataatatta tggaagttaa
    11581 attttcggca atatctgaaa atgaaagttt agcaagagtt atagtagcat cctttgcagc
    11641 taagttagac ccaacagtag atgagttagt agatataaaa actgctgtct ctgaggctgt
    11701 tacgaatgct ataatacatg gatatgaaga agacagttct aagtttgttt tcttaagatg
    11761 tgagatagaa ggaaatacta taaagattat agttgaagat gaagggtatg gaatagaaaa
    11821 tgtggagaag gcaatggaac cactttatac atcaaaacca gaacttgata gatcaggaat
    11881 gggattcaca gtaatgaaaa gctttatgga cgatgtagag gtaagttctg taaaagataa
    11941 tgggactaga attgaaatga ctaaaaaaat aaatgtgcct aaataaagtc caaaagtgag
    12001 gttttattat ggaagtaact gttgccagag aagaaaaaaa acctcttctt agtcatgaag
    12061 agactctaga acttattgaa aaagttcaaa atggagatga agaggcaaaa gaaatattaa
    12121 tatcaagcaa tttaggtcta gttaggagcg ttgtatctag atttttaaat ataggatatg
    12181 atagagaaga tttatttcag ttaggttcta taggcttaat aaaatcaata tataaatttg
    12241 acccaaaatt taatgtaaaa ttctctactt atgcagtacc aatgatttta ggtgaaataa
    12301 agagatactt aagagatgat ggtatgataa aagtatcaag gtcgttaaag caaatagctg
    12361 taaaggcaaa gatggaaagt gaagctttaa ctaaaaaatt aggtagagag ccgagtatag
    12421 aagaattagc taaagcaata gatgtagaaa aagaagattt agtaatggct atggaagcta
    12481 atttcaatgt tgagtatttg caaggagtta ttcatgaaga ggaaggttct ccaatatgcc
    12541 ttattgataa aataagcatg aaaggagaaa gtgaagaaga aaaagtagtg gataacatat
    12601 tgttaaaaga catacttgga agactggata agagagagcg tcaaataata gttctcagat
    12661 actttgaaga catgactcaa agtgaaatag gagagatgct caatatatca caagttcaag
    12721 tatctaggat agagaagaaa gtattgtcta agttaaaaga atatatatcg tgataatact
    12781 tatgatatgt agaataacaa aatataataa atatattact aaaataaaat gtaataactt
    12841 tttatgttaa gtttttaaat tattaaaaga tattaaaata ataaaaagca ttaaaaaagt
    12901 aaaaatataa aaacaaggtg tctttatgga aaaatagatt ttttctttat taggcatctt
    12961 ttttattttt tgaccaaact ttagatagtc aaaataaata ataggtttat ttttttaaaa
    13021 ttaacaaata atactttaga gaggtgttta gtatggataa aaattataaa aaatatgtag
    13081 accaaataag cccaaaacct acatatttaa aaaactatac acttgcattt attgtaggtg
    13141 gaattatttg tatgataggg caagcaataa atgatttgta tatgaaagtt ggaggtcttg
    13201 ataaactagg tgctagttct gcaacatcaa taacattaat atttataggg gcatttttaa
    13261 ctggattagg agtttacgac ctaataggaa aaagagctgg agctggttct ataattccaa
    13321 taacaggttt tgctaactca atagtatctc cagcaatgga gtataaaaga gaaggctatg
    13381 tattaggtgt aggtgcaaac ttatttaaaa ttgcaggacc agttttagtt tatgggatag
    13441 gttcttctat tttatgtgga attatttatt atatttttaa gatgttttag atgattttta
    13501 tttaatttaa attttattaa ttatgaagaa ggtataaaat ctgaaaataa ctactaaagt
    13561 aactaattga aaaataattc tgcagattta attaatattt ttaattaata ttaaaaattt
    13621 gaatagtaaa atgcaaaatt aaaaaggtgt gaaaatatga aaaataaaag aattggaaaa
    13681 agaacagtca agttagaaaa taaaccaacc ataatatcaa ctggaactat agtaggtcct
    13741 aaggaaggtg aaggaccact aaaggactat tttgatatga ttatgactga cgatttatat
    13801 ggtgaaaaga cttgggaatt agctgaaagt aaaatggtag agactgcatc tcaacaagct
    13861 atccaaaagg caggtaaaaa attgtcagat gtaaattata tgctaggtgg cgatttaata
    13921 aatcaaatag ttccagcatc ctttgcagca agagaactgg ctattccatt tttaggtata
    13981 tatggagctt gttctacaat ggcagaaggt ctttgtatag gttctatgct tatagatggt
    14041 ggttttgctg atttagtact gtcaggtaca tcaagtcact attgtacagc agaaagacaa
    14101 tttagattcc cacttgaact tggaaatcaa aaaccaatga cagctcagtg gacagttaca
    14161 ggagcaggaa gtgtattatt agctcctaat ggagacggtc caaaagtaaa gtatgttact
    14221 gttggtaaag taatagatga gggtattgat gatggtaata atatgggacc agctatggct
    14281 ccagcagcaa tagatactat ctactcttac tttgaagata ctaatgatga cccaaatagc
    14341 tttgatataa ttgctactgg agatttaggt actcttggaa aacagatagc agaagacttt
    14401 ttaaaagaaa agggtgtaga tatatcaaaa gtttacactg actgtggtat agagatgttt
    14461 aaccttaaag aacaagatgt ccattgtggt ggaagtggat gtggatgttc agctacagtg
    14521 tttgcaggat atatatatga taagttgaga aaaaaagaat ttaacaaagt aatgcttgta
    14581 tcaacaggag cactactttc accgacaagt acactacaaa agcagacaat accatgtgtt
    14641 gcacatgcag tagtaatagt aaatgagtag gaagtgatat aatatgttaa tggactatgt
    14701 aagagtattt attgtaggtg gaattatatg tttaatagcc caagtaatga tggattattt
    14761 caaaatgcaa actccatatg ttatggtaac gtatgtaaca actggtgtag ttttaacttt
    14821 tctaggactt tatgagccat tagttaagtt tgcagcagca ggtgcaacag ttcctataat
    14881 aggatttgga tattcacttg ctacaggagt aataaaatca attcaaagtg atggtttttt
    14941 aggtattttt ttaggaggta caactgctac agctggtgga atagcagcag ctatattctt
    15001 tggatatatt atgtcagtag tatttactcc aaaagcaaaa ccataaatct ttattaaaag
    15061 aataataagt ttttgaaata ataacttttt ataaagcagg tggatatctt ttagattcca
    15121 cctatttttt atataaagaa gcgtttacat aattgaagtt gacaattatt tacaacatag
    15181 tgtagtatta tattatcaaa ataaaatgaa gtgaagtagg ggataaagat ggtaactatt
    15241 aaagatattg caaggaatct aggtatcagt tattcaactg tttctaggtg cttaaacaat
    15301 aatcctaatg tatctgaaaa aacaaaaaag aaagtggtag aagaggcaaa tagactaggg
    15361 ttccatttta atgtaaatgc aagaaattta gcaaaaaaag aaactaacag gataggggta
    15421 atcttttcaa ataattttaa tcatcaagat actagaaagt tttttagtga tataatggat
    15481 agttctataa attctattga gactaataaa tacgatttta taatacagcc taataataac
    15541 attagtggtg atagtaatgt atataaaatg gtaaatggac aaatggttga cggattagta
    15601 attgtgtctc aatcaataaa aaaagaagag tatgattttt taaaagacaa taattttcca
    15661 catgtattta tatattttaa accatctttt ataggagaag tagacaattt cttttgggac
    15721 gataacgtgt atggaggtta tatagctacc aagcatttaa ttgaccatgg tcataaagat
    15781 ataataacag taacttcaga tgataagagt tcaaaaatgc atgaagatag aaccaaggga
    15841 tatttgaatg ctatggaaga ggcaaattta aagacagaag tcattaaatc taaaatggat
    15901 tttgaatctc aagttgaatt tttagaaaaa tacattgata aaataaaaaa agcttcagct
    15961 atatttgtac aacaagatgt accagcgatt tctataatac aagagttaaa aactacttat
    16021 ggtataaatg taccagaaga tatttccata atagggtata ataatattga attaatatca
    16081 tattttaggt cgcatttatc aacaatagat gacccaagag agcaagttat aaaaaatgga
    16141 gtagatagtt tagtaaatat aataaataaa aagagtacag aacacattcc aagaaaatta
    16201 tatccaaggt taataatacg aaatagcgtt aaaagaataa agtagtaagc ttttaagtat
    16261 caaacgttta ttaaaaatat tatagattat gtgaagtttt ttaacaaata atataaataa
    16321 aaattaaaag gagtaggtgt atataagtac attgtactcc tttatttatt gtactccttt
    16381 atttattgag atagttttta taatatgaat gaataatttt attcaattat t
//
LOCUS       DAEORZ010000093        16146 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEORZ010000093 DAEORZ010000000
VERSION     DAEORZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.093"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(168..701)
                     /gene="pyrR"
                     /locus_tag="KRH39_002582"
     CDS             complement(168..701)
                     /gene="pyrR"
                     /locus_tag="KRH39_002582"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBG8512475.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(1061..2008)
                     /locus_tag="KRH39_002583"
     CDS             complement(1061..2008)
                     /locus_tag="KRH39_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG8512476.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(1977..2423)
                     /gene="lspA"
                     /locus_tag="KRH39_002584"
     CDS             complement(1977..2423)
                     /gene="lspA"
                     /locus_tag="KRH39_002584"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG8512477.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(2733..3512)
                     /locus_tag="KRH39_002585"
     CDS             complement(2733..3512)
                     /locus_tag="KRH39_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG8512478.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(3567..4049)
                     /locus_tag="KRH39_002586"
     CDS             complement(3567..4049)
                     /locus_tag="KRH39_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG8512479.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(4315..5736)
                     /locus_tag="KRH39_002587"
     CDS             complement(4315..5736)
                     /locus_tag="KRH39_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG8512480.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(5882..6652)
                     /locus_tag="KRH39_002588"
     CDS             complement(5882..6652)
                     /locus_tag="KRH39_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8512481.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELSNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(6657..7859)
                     /locus_tag="KRH39_002589"
     CDS             complement(6657..7859)
                     /locus_tag="KRH39_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG8512482.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
     gene            8725..9471
                     /gene="cdtR"
                     /locus_tag="KRH39_002590"
     CDS             8725..9471
                     /gene="cdtR"
                     /locus_tag="KRH39_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBG8512483.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
     gene            9860..10042
                     /locus_tag="KRH39_002591"
     CDS             9860..10042
                     /locus_tag="KRH39_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512484.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
     gene            10260..10403
                     /locus_tag="KRH39_002592"
     CDS             10260..10403
                     /locus_tag="KRH39_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512485.1"
                     /translation="MYVYYFESPEKFAFNKVIRTKNQNEISLEKFNEFKETIQDKLLP
                     YNR"
     gene            10378..11945
                     /locus_tag="KRH39_002593"
                     /pseudo
     CDS             10378..11945
                     /locus_tag="KRH39_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(12597..13598)
                     /gene="trpS"
                     /locus_tag="KRH39_002594"
     CDS             complement(12597..13598)
                     /gene="trpS"
                     /locus_tag="KRH39_002594"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBG8512486.1"
                     /translation="MSEKKVILSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(13621..14319)
                     /locus_tag="KRH39_002595"
     CDS             complement(13621..14319)
                     /locus_tag="KRH39_002595"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBG8512487.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELLNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     misc_binding    complement(14362..14602)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14693..16096)
                     /locus_tag="KRH39_002596"
     CDS             complement(14693..16096)
                     /locus_tag="KRH39_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG8512488.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
BASE COUNT         5733 a         2582 c         1711 g         6120 t
ORIGIN      
        1 tcattatttt acattttacg acatcaaaaa ctcaaataaa agagaataaa aactacatta
       61 agactcacat ccataaaatt ataatattta ttttgtgcaa tctatgtaag ctttatcctc
      121 ctccctgact attcaatgct aagcttttat ataatactta aaaataatta ttctttgatt
      181 gtaactgaat cttctccatc aatttctaat aaactgacag atataatttc atgtctagaa
      241 gtaggtacat tttttcctac ataatctgct cttataggca actctctatg ccctctgtcc
      301 actagcactg ctaattgtat tgatttaggt cttcctatat ctataatagc atctagtgaa
      361 gctctcacag ttcttccagt gtataaaaca tcatcaacta aaactactgt cttatcattt
      421 acattaaaat ctaaataagt tcctttaact acaggttcaa catgaatttt ttccaaatca
      481 tctctataca aagttatatc aatatctcct gtatctactt ttgttccttc tatttgttct
      541 attttcttag aaattctatt tgcaattggc acacctctag tctttattcc aactaaaact
      601 agattttcta cacctttatt tctttcaatt atttcatgac ttattctagt aattgctctt
      661 gctattgcct tttcatccat taattgagct ttttctacca tatcataatt attgtcaaag
      721 atataatata tctatttgac aaatctactc ctttctttta attttttata aatttatttt
      781 tatttaaaat ttcaatttaa acaacaaaaa aacttctcat cacaagacaa gaagctctta
      841 tataaaaaat atacagtgca atttagcaat tgtcttacga tatctctgta tcgtttaaag
      901 ataatttttt aatctatcaa atcaaattat atatttatat ttcaaatttt tcaagctttt
      961 ttaaacaaaa tactttaata taagatattt tatttaaatt tcactaaatt tatttactta
     1021 tataaaaaca aaaaatgatt tataagtatt atacaaatgg ttattttatc tttttaataa
     1081 cttccttaaa atactcaggt aattctgaat taaattcgat atattcctta gtagtaggat
     1141 gtataaatcc caacttctta gcgtgcaaag tttgtccttt taatttaaat ttagtttctt
     1201 ttggaccata tacataatca cctaaaagtg ggtgatttat agacaaagca tgaactctta
     1261 tttgatgagt tctaccagtt tcaagcctag ctctcatatg tgtaaactta tcaaatcttt
     1321 ctacaacttc aaagtgagtg attgcatttt ttccatcttt tactacagcc atttttaatc
     1381 tatccttagg atttctccct ataggtttat caacagttat tttatcttct ttaaccacac
     1441 catagcatat aaattcatac tccctagtta tagtatgttc ctttaactgt tctgctagaa
     1501 aattatgtgc ataattgttc ttagctatca taagaagacc agaagtatct ttgtctattc
     1561 tatgaactat accaggtcta ataactccat ttattgatga caacttatct ttacaatgat
     1621 ataaaatccc gtttactaac gtattttcat agtttccagg agcaggatgg acaaccatat
     1681 cctgaggttt atttattatt aagacatcat catcttcata tacaatatct attggaatat
     1741 cttgaggtaa aacttctaat actttaggtt ctggtatctc tataacaata ttatcttcct
     1801 ctttaaccag atatctagct ttttctactt tattattaac ctttactttt ccatctttta
     1861 taattttttg tatataactt cttgacatat cacctaattg ttctgctaga taaatatcaa
     1921 gtctaacttc ttcttcctct tcaagtacta aaaactcttt tacttcatcc attatatcac
     1981 ctacttttac tttcaaaaaa taaaacatat atacataaaa acacagttcc tacaactaca
     2041 aatacatcag ctatattgaa tacatattcc catataattc taaagtcgaa gtaatctact
     2101 acaaaaccta atcgtactct atcaattaga tttcccaatg caccagatat aattaatata
     2161 attcccaacc ttccaaatat atgtactttc cttgtattaa gatagtatag tccaaatact
     2221 gttgcaagta atgcaacaat tataaatatc cattgattat tttgtaataa tccaaatgct
     2281 gcacctctat tttcgacata agttaaatga aatacattat ttattattgg tattgttgat
     2341 acatccacca aattattcaa tacccatatt ttagacagtt ggtctaaacc tatgagtaga
     2401 attattatta atatatatag caatgatttt tccccctata aattatttaa aaaccaattt
     2461 tatttgaagc acactattat tattatacaa aaaaaatgct ctaaagagca actttattaa
     2521 aattgtattt tcttaataaa gctagaacaa aatttaaaat agtactactt tttttatatt
     2581 gtagatttct acatatttaa aatcatatca ttatgtataa aaaatttaaa aataccatac
     2641 taatcaatat tttttgtgta ttttatagat taaagattaa tgatgtctgt aatttaaact
     2701 tacaatcacc actaatcttc aaacaaatat ttttactttt gcacttgctt atttttatct
     2761 aaatgttgta taagttcaga tactgttacg aatttatatc ctctctcttt taatttagga
     2821 attactactt ctaaagcttg tatagtttgt gatttatcat ttcttttagt gttataatca
     2881 tgtaaaagta ttatattacc attttggacc tttgtaagga tagtatttat aattgcatct
     2941 actccagggt tagaccaatc tctagggtct aagtttgacc aaagaattat attcatgcct
     3001 ttagaaacta taatatcaca gacactttta ctcatggctc tatatggagg tctaaatatc
     3061 tttggttcta ctcctattac actttttata atattttgcg tatctgttat ttctttttca
     3121 acactactat aactattttt tgcaacattt atatgtgtaa atgtatgatt ccctatttca
     3181 tgcccctcat catattgtct ttttaataat tctttgttat attctacttt ttctccaact
     3241 acaaaaaaag ttgcttttac atcatttttc tttaatatat caagtacttg tggtgtaaaa
     3301 tcctcatctg gaccatcatc aaaagtcaat gctaaaattt tttcatcctc atttcctttt
     3361 tttattatta catcttcata atcgtctttt atattgtcat ttaaactata tgcttctttt
     3421 gaactatctt gattatgtgt attatgatta tatgtatata cacctactat agataataaa
     3481 catatacata taccccatat tattttcttc atgtcattac ctcttttcta attcattcta
     3541 gcaatatatt ttataatgtc agttttttaa tctctcaata ttccattttt atttaaatca
     3601 gttaaatttg ttaggtcttc tgaataaaag ttagaatctt ctacatcagg gttttgcatt
     3661 gcatcaccta ataaattatt ttctttcttt gcacaatcat tacataaact tgtatctggc
     3721 aatatatcca atctttctaa atctatttga ttatgacata aatcacaaat cccatatgtt
     3781 ccattctcta ttttagataa tgctaagtct atttgataca atcttccctc ttcatgattt
     3841 attagactgt tttgcatatt ttgcatatat aaatctgttc ccatgtctcc tatatgatta
     3901 tcataactag ataattctcc tgatgaatac ttttcacttg tatgtttagt tgtatctcca
     3961 aataaagtat tatctttcat atcctctact aaattagtta aattttctct ttctttcaat
     4021 aatttatctt tatatttttt agtatccatt tttaacacct ctaattttaa tatttacatc
     4081 ataaaaatag tatgtggaaa agtaaattaa acatacaata aaaataagat ttgattcgaa
     4141 aaatggaatg tctcaaaatg aaatttttag ttcatttaac cctctttttt atatgaaatc
     4201 aaatatattt ttatcctttc agcaataaaa aagagactta tctctatttt gagatagtct
     4261 cttctttgaa cataatttaa atataatttt ttaattttta tagttattat tttactaact
     4321 tgccaattta tgattatttt gttctttaat atgttttgct tttttaaaga ataaaagacc
     4381 aagaattatt gctacaataa cacctattcc attagatata ttaactggta gtctaaatcc
     4441 ttcttttgct tgcattatat aactaaatgt tactacagtc ataaaaagtg ctggtaatgt
     4501 agttatataa tggtttttat ttgctttgac taaatatgca gaaccagtcc acaacattat
     4561 cattgcaagt gtttgatttg cccaagagaa atatctccaa attatattaa aatctataaa
     4621 tgtaagagca acaccaactg cgaataatgg taaagctatt aaaaatctat ttttgactgc
     4681 atcttgatta taattcattg catctgctat tgtcagtcta gcacttctaa atgctgtatc
     4741 tccagaagtt ataggacatg ccacaactcc aagtatagct aaagctcctc caataggccc
     4801 cattaatcca acagatactg tatttactac tgttgctgct ccaccatcat taaatatcac
     4861 attaagttga ggtatgccat ggaaatatga catggctgct gctgcccaaa ctaaagctat
     4921 tataccttct gcaaccattg acccataaaa tactggtctt gcctcactct ctttctcaac
     4981 acatctcgcc ataagtggtg attgagttgc atgaaaacct gatattgctc cacatgctat
     5041 tgttataaat aagtatggga ataaaggtgt tccatttgga ttcatatttt gtaaatttat
     5101 atttggtatg ttatacccct ttattatcaa tcctccagct acacctactg ccataattaa
     5161 taatgctgct ccaaataaag gatatatttt accaatcaat ttatctattg gtaaaacagt
     5221 agccataata taataaagta ttatcactcc aacccatatt tgtgcgttta cacctgtcaa
     5281 tgtctttaat aatcctgctg gacctgttac aaatacaact ccaaccaata ctaaaagaat
     5341 aacactaaat attctcattg ctatttttgc accatttcct agataaagtc ctaccaactc
     5401 tgatgcagat gtacctttgt ttttcattga tagatatcct gaagcaaaat cgtgtactcc
     5461 accagcaaag attgttccaa acacaatcca taaaaatgct gatggtccaa ataatgcccc
     5521 ttgtatagct ccaaatatcg gacctaaccc tgctatattt agaaactgaa ttaaaaagat
     5581 tctcttccat ttcattggca cataatctac tccatcccta caagctatag ctggagtttg
     5641 atttttgtca ttgattccaa aaaccttttc tacaaaagta ccatagacaa aataacctac
     5701 tataagtact actattgaac ctaaaaagct taccataaat tcatcccctt agtttttata
     5761 taattgcttt ataaattttt atgagtttaa gaaaactatt tccagttcaa atagtaatac
     5821 ttttttatgc taatataaat attaattaca ttaagtgcat atttaagtac atgaaatgac
     5881 tttatatatg cattaacagc ctaaattctt ttactttgct tctacttaca gttacttcat
     5941 aatcactatt ttttaatttt aatatgtatg tattattaaa ccatggtata atttctttta
     6001 ctttggttaa gtttacgata taagacctat gtgttctaaa aaatgtttta tcatttataa
     6061 gattttcgac ttctgatatg ccttctttta atataaactt ttctttctca gtatatataa
     6121 atgtctgcct ttcattagct tcacagtaat atatatcatc aatatctatt acaactaatt
     6181 tatcaccttt ccaaaggcta attttatgtg ttatttcttc tatctcctga tttactgctt
     6241 ccttcttata tttgtataaa ttactattta cattagatag ttcaatatca ctcatctcac
     6301 ttttttctaa tttattcagc atagaaataa ttctctcttc agaatatggt ttcaatatat
     6361 aatcaaatac atccaaagaa aaagcatcta ctgcataact ttcatatgct gttataaaaa
     6421 caattttagg tcttgattta aactgattta atgtttttgc aagtagcatt ccatcaagat
     6481 gaggtatatt tatatctaaa aaaattacat ctattttatt ttcctgaata aagtctaaca
     6541 cctctatacc attggtaaat tcacttacta cctctattcc acttttattt tcaataaaat
     6601 atctcaattc ctttcttgct ggaaattcat cttccactat tatagctctc atacttctac
     6661 tcctttacat aaaatactat caaagtccca ttatctattc ttcttatatt aagccctctt
     6721 ccatacatca gtttaagcct taaatgtaca ttataaagcc caatcttatt ttctggcata
     6781 ttatcttgat atacattatt tataatttcc tcacttatac ctataccatt atcctctatt
     6841 gatattctca cagtattttc taatgaatac ggtaactttt ttatggatat tttcactaca
     6901 ccagctctac cactttctaa aataccatgt attattgcat tttccactaa tggttgtata
     6961 atcaaacttg gaatctttat atttacatct tctatatcat aagatacagt tagtaattct
     7021 ccaaacctag ctttttctat ctctacaaaa gatttaactt gttcaatctc tttatttata
     7081 tcaattaagt tatcactata ttcaagatta tatcttagat aagttgataa atttattatt
     7141 aactctctag ccttatctgg actaaatctt ataaatgaag ctattgtgtt tagggaatta
     7201 aacaaaaaat gtggattaat ttgtctttga agtgctttta tttctgcttt atttctcgct
     7261 tctatatctt ttttatcttt ctcaatcccc tctactatag atataacaaa tcctattcca
     7321 atctgcccaa ctattaaagg taaatatatt ccaccgatta tttgaattgc taaagaatgt
     7381 ggatatgata ttaagtatat aagtaatata gtaaaacttt cagaaatcat gcctaccaat
     7441 attccataca ttactctatg ttgttttggt atcctcttat atagaaatcc tgataataca
     7501 cctgctagta tacttgataa aagacatgga acagatgtta tccccccaat atccataaaa
     7561 tatctatgaa tccctgaaaa aactcctgct gtaataccta ccatcggacc aaataaaatg
     7621 ccacctgata ctatagatat tattctagtg tttactattg accccatata atttatacca
     7681 ctataagttc caaatatggc taaaaaagta aaaactaatg ctatacagac caagtcatta
     7741 aaactatatt cttccttttg aaatatctgc ttaaatattt ttaactttgt tattagaaga
     7801 aacaatacta caataaggct tgtcttttct attaatgcga ttaaaattga tgttttcata
     7861 atcccccttt aaaaaataaa tttttacaca ctctaaattt attatggttg ttataaatta
     7921 catatttgaa gtaatcctat cacaaaactt tccacattta atcttttaat taaataagaa
     7981 atagttgtac ttctattaac aactaatact agctagcata agcttgtaat aacttgtagt
     8041 tattattaac tttacaattc tagtatttaa ttttatactt tagaaatctc ttttcttaga
     8101 aataatactt tcgaatatca tataatacaa tattatgtag attcttactc atacaacata
     8161 tttctttaaa aattttatac tattctttgt tcaaatatct catttgattt aattgtgtta
     8221 aacacacatt atcatctctc tgttatatat ttattatata gccgtatatc gataaagtaa
     8281 ataaatccca tatttaaaat aatatgtttg acaataaatt attataatgt atatttacca
     8341 taataatttg tcaaccttga aagttacttc tataattatt gttaaataat tcttctaaaa
     8401 aaaataccta taaattttat tttctgaaat acatagttat aactattttt atcacgtata
     8461 agaataaaaa ttccagaaaa tttttattat ctaagttaat ataaaacata ttatttaata
     8521 taaataaatt ttttataata atatattgat ttaatttact ttattattgt aaactataag
     8581 tataagatat actatataaa caagcataaa tataccttaa ttctaactat ccttaatctt
     8641 atttatctcc tagtgttatt ttatgtattt tctataaaat acttttgttt ttttactttt
     8701 gaatttttta taggagggta tttagtggat atattaatct ttgataatga tgtttgtttt
     8761 ggaatgaaac taaaagaaaa aataaataat attttaataa aagaaggttt tgataatgat
     8821 gttgtaagat tatatcataa tgcaaatttg ttattaaaag agcttactga aaaaaataaa
     8881 gtaagaatat attttatagt tgtagacgca aaatataaga taagtaatga attatgtgat
     8941 ggattatgga tagcacaaaa aattagagaa tcagattata ttagtccaat catatttctt
     9001 acaaatcata tagaaatgat tttagggatc ttcgattata ggttagaagt tatggacttt
     9061 atactaaaac atgatatgga aattgctgaa agtaaaataa aagcttgtat taaaattgct
     9121 cacaaaagat acattaaaga aaaaaattat cgctctaatt tttttactat ctattcagat
     9181 ttctcactat ggaaaatttc atttgatgaa gttatatatt ttgaaacaag cactattcca
     9241 cataaaataa aattagtgac tacttctaga atatttgaat tctataaaag tttaaagtct
     9301 ttaccagatt tagatgcatg ctttatccgt gtacataaat cttttgtagt taataaatat
     9361 catattgttt ctctcgacct aaagaaaaat aatattaaaa tgagtaatgg ccatatatgt
     9421 cgtatatcta atacatatag aaatatttta aagaatatta ttaaaacata ataaatagaa
     9481 aaaggagatg tctcaagata aaaatttgaa ccatctcttt tttatacaaa aagagtaatc
     9541 cctaagaatc ctctatataa taatcatatt gtgaaaaaac acatactgac tatatcatat
     9601 ataactacta ttagatatta taacaattgt tcctagatta tgattcgata agatagctca
     9661 ttaaatcata aaagtacgaa atagtctctt taaaatattc agttgttatt ttgtactgac
     9721 atatcatata aatacatatt tttatgatat atacctacat attttatgaa atttatataa
     9781 aaaattctta tttagattat ataatctaaa taaattaaaa ttcaaaagtt aactaaacta
     9841 atatttgtag ggagaataaa tgaaaaaatt taggaaacat aaaagtatta gtaattgtat
     9901 atctatattg ttgatattat atctaacttt aggtagtttg ttacctaata acatttatgc
     9961 acaagattta caaagctata gtggaaaagt ttgtaatact acttacaagg ctcctataga
    10021 aagatcagaa ggattttttt aaagataaag aaaaggctaa agaatgagaa agaaaagatc
    10081 aaaaacttga aaaatctgaa aaagaagtat taggatcata taaaaaagat tctatagaga
    10141 taagcaaata ttctcagaca agaaattatt tttatgatta tcaaatagaa gcaaattctt
    10201 gagaaaaaga atatagagaa cttcgaaatg ctatatcaaa aaataaaata gataaaccta
    10261 tgtatgtata ttattttgaa tctccagaaa aatttgcatt taataaagta ataagaacaa
    10321 aaaatcaaaa cgaaatttca ttagaaaaat ttaatgagtt taaagaaact atacaagaca
    10381 aattattacc atacaacaga taattcaact gctgttcaaa atagcaatgg ataatcatga
    10441 aatactggat taagtataaa caaaggagaa tctgcatata taaatgcaaa tattagatac
    10501 tacaacacag gtactgcacc tatgtacaaa gtgacaccaa caacaaattt agtgttagat
    10561 ggagatgcat tatcaactat caaagcacaa gaaaatcaaa ttggcaataa tctatctcct
    10621 ggaaatactt atctcaaaaa aggattttca cctatggctc ttaacacaat gtatcaattt
    10681 agttctagac tgattcctat aaattataat taattaaaaa aattagatga tggaaagcaa
    10741 attaaattag aaacaacaca agtaagtgga aattttggta caaaaaatgg ttctggacaa
    10801 atagtaacag aatgaaatag ttggtaagac gatataggtc aaattgacag tatttctgta
    10861 tctattatat tagatacaga aaatgaatct tacaaaagaa gaattactgc taaaagttta
    10921 caggtcccag aagataaaac acctgaactt acaattggag aagcaattga aaaaactttt
    10981 ggcactacta aaaaagatgg tttgttatat tttaataata taccaataga tgaaagttgt
    11041 gttgaactca tatttgatga taatataggc aataagatta aagacagttt aaaaactttg
    11101 tctgataaac aaatatgtaa tgttaaactt gaaagaggaa tgaatatact tataaaaaca
    11161 ccaacttact ttactaattt tgatgattac aataattacc ctagtatatg gagtaatgtt
    11221 aatactacaa atcaagatgg tttaaaaggc tcagcaaata gattaaatgg tgagacgaag
    11281 attaaaatac ctatgtctga actaaaacct tataaacgtt atgtttttag taaaattcaa
    11341 aaaatccttt aacatataat tcaataattg taaatataaa agtaaaagaa gaaaaaacgg
    11401 attatttggt actagaacaa ggatatacaa aatttagtta tgaatttgaa actactgaaa
    11461 aagattcttc taatataaat atagcattaa ttgatagtga tacaatatac ttagataact
    11521 tatctattac agagctaaat agtactcctg aaatacttga tgaaccagaa gttaaaattc
    11581 caactgttca agaaataata gttacacata aaatatattc tgcagatttt aaatttaatc
    11641 caagtacaat aaaggcttat ataaatggta tgtattttac accaacataa actaataaag
    11701 aagctctcga ttatgtccaa aaatatagag ttgaagctac tttacaatat tttggattta
    11761 aagatattga aactaaagat aaagaattgc gtaattattt aggagataca aatcaaccta
    11821 aaactgattg tgttaatctt aggagttatt ttacaggtgg agaaaatatt atgacataca
    11881 agaaattaag aatatataca attactcaag atgatagaga gttattggtt cttagtgttg
    11941 attagtatca aatgatttaa atttatcccc acaatacaat atgtatttac cttataaagt
    12001 attaaaaaat taactttata aggtattttt tatttaaata ttatcttctt ctaatatatt
    12061 cttttttaga actatattgt attaaaaaga taattatctg tatcatatca cctatatata
    12121 aacataaatt catttaatta tataaattta aaaaatattt ttttgatata ttattgacaa
    12181 ttttcattat aagagttaaa attaactcat aaattacata ttgggtataa aatacaatgt
    12241 atgttagaag ttgtttcaga aggctatggt tttctgattt ttttatgctc agtagtgata
    12301 tgcaaaaaag acaaagttga catcagttaa cttttacctt tattctactt tccccaaagt
    12361 aagaataatc taaccccttt gagatgagca aaagctcatc tttttttatt atcaaaagat
    12421 gctgtctcaa aatagagata atcctctttt tcattgttga aatgattaaa atatatttta
    12481 tttcatacaa aaaagagtgc ctcatgaaca aaagttcact ttgagactct ctctttcata
    12541 tcatatacct ttttaataac tttaaaattt atttatcaag taaagatagc taataattag
    12601 tatcttctct ctataaatcc aactttttta taaacttttc taagagtttt tctagcttgt
    12661 ttttcggctt tttctgctcc catactatat attttttcta aataatcttt attattaaga
    12721 aggtcaacat atttttctct tataggtctt aaagcttctg atactacttc agccacatct
    12781 tctttaaata tgccataacc ctttccttca tacatgttta caatctcttc tacactcttt
    12841 ttacttaaat tagaatatat atcaattaga tttttaatac ctggttgttc atcattgtat
    12901 tttacaacac ctaaagaatc agtaacactt ctttttatct ttctctttat agaatctgaa
    12961 tcatctgcta atagtataaa tgcattttca tttgaatcgg atttactcat ttttttagtt
    13021 ggctcttgca aactcatcac tcttgctcca ccttttgggt agtatccttc tggaactaca
    13081 aaagtaggtg aaaatctatt attaaatcta tttgctaaat cacgagctaa ttctaaatgt
    13141 tgtttttggt catctccaac tggaactagg tcagtttgat acaacaatat gtcagcagcc
    13201 ataagaactg gatatgtgaa taaacctgca tttagatttg cttcactctt ttgtgattta
    13261 tctttgaatt gagtcattct acttaattca cccatataag tcatagtatt caatacccac
    13321 ataagttcaa catgctcttt gacatgtgat tgtataaata tagtattttt ttctgggtct
    13381 aatccacaag ctatatattg agcaagtaat tctatagtat tggctcttaa aactttaggg
    13441 tcttgaggta ctgttattgc atgcaaatca actatactat aataacaatc ataattgtct
    13501 tgaagttcag tccagttttt tatagctcct aaataatttc ctaaagtcat tttgcctgat
    13561 ggctgtgcac cacttaatat aacttttttt tcactcattt ttttgtacct ccttatatat
    13621 ttatattgat tttaacatat ttaaaactat gtctttagag ttattggcag catcatgtac
    13681 aaatacatca taagtaacat cagcacttcc atctgcctta tcagacattg ctcttattat
    13741 tacaaatgga gtattattta aatagcatac atgagctata gctccacctt ccatttctcc
    13801 acaatatcca ccaaaatcat ttaataattc ttcttttaat tctttagaat ttataaattt
    13861 atctcctgta actactcttc cttttaatac tttatgagtc tttacttctt ccacagaaga
    13921 tttgtaagca gcatctataa gtctttcatc tgctttaaat acagaagtct tcattcttgg
    13981 tataactccc ttagggtctc caaatgctgt agtgtctaca tcatactgta tagcatcagt
    14041 tgatattact atatcattaa catcaagttt ttcgtttaaa gctcctgcaa cacctgtatt
    14101 aacaatagca tcaaccttaa attcgcttat tagtatctgg gcacaaagag cagagttaac
    14161 cttacctatg ccacacttta ctaacactac attcttacct tccaaaattc ccttataaaa
    14221 ttctaagcta gcttttttta tagtctctct aatatccatt aaatcaacta aaatactaac
    14281 ttcttcatcc attgcaccta taattcctat tacgttcatt tttcctccta aattttctat
    14341 attaaaaaat ccgtcctaaa tcaataggac ggatatattt tccgtggtac cacctaaatt
    14401 tatatacact caaaattgaa tatatatatc ttaatatatg ctataacgtg catacacgat
    14461 atcaaatact ctgtaatctt aaattacatt tcattttact cctcaaagac ccattcaata
    14521 aatacttttt attaagttct cagcatcctt aattctctgt gcaaaaatat atttatttac
    14581 tatttcttat ctcaggattt aatttattaa tttctttatt ttataatatt ccttacacta
    14641 taaattgtca actatatatt ataatatcca aaagaaattt ttaaaataca ttttatttag
    14701 ctgttttctt ttcatatcta ttccataacc atagaccaga taatataact agaagacctc
    14761 caccaatcat ctctatctca tatataacat tttcagcaaa tgtaggcata actggtgctg
    14821 cagataataa tatacccact acagttacaa ctaaaaccat ttttgctaaa cctaggcaag
    14881 tacttgttga ttttgccata gtataaggtc taatttcatc tggtctctct tttcttagtt
    14941 taatataaga agcatataac aatacatatg ggaataatgc agttaatgct gtcatagtta
    15001 caagaacatt gtatatagca tcaacagaag gcattaatgt agtcaccaat agtattatag
    15061 taactattat tgcttgaagt ataactgctt tagaaggtat cttgtgttca tttacctcta
    15121 ctaaactatc tggaaatatt ccttttttaa cactaccaaa taacattttt attggtgatg
    15181 ctatgtaaag aactattgct cctaatactg aaaaagctat accaaaagct attaattgaa
    15241 ccaaccaaga accaattcct aaagacgcac aaacagcaga aagtgcatct aatacacctt
    15301 tagaagcagt aatttggtct ggtggtatta tcattgttat tgcaattgaa cctaatacat
    15361 ataatgaagc tactattcca gctgatatta atattgcctt agggaagttc ttttcaggct
    15421 tatctatttc tgttatgaag ttagcagcag tctctgctcc tgctaaacca aacattatag
    15481 ctgatatagc aacaaatgca tccatattaa gttttggagt cattgaagct attgtgtatg
    15541 tagaagctat atcatgtttt ttcaacacaa atactgctac aaatgaaaat attataagta
    15601 tagcacttgg tattgttgaa cctaatgcac ctgtattagt aaatattttt gtaaaactta
    15661 ttcctttagt tgctataaga gataatgccc agaatattat taaagataat ataagtacaa
    15721 acatcttatt atcagcaagg gctggatttc ctattgtata agaaacattt actgctaaga
    15781 aagttaaaaa tgaagaatac caaaatattt tagctgtcca gtttaaccaa gaaactaaaa
    15841 atccatactt ttctccaaag gcttccttaa cccaatcgta aagacctcca tcaccttttt
    15901 gatatgtaga agctaattca gcacaaatta aagctcctgg tacaaagtat ataaatgaga
    15961 ataataacca cataggtatt gcacctaatc ctaaaccaaa gcttgatgct gtagatttag
    16021 ctatccatct tataccatat aaagctgata cattcataaa taccaaactc cataggccta
    16081 tttttttctt ttccatccta atttaatttc ccctctctat taattatttt aaatttaatt
    16141 tcaaaa
//
LOCUS       DAEORZ010000094        16009 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEORZ010000094 DAEORZ010000000
VERSION     DAEORZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.094"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            76..792
                     /locus_tag="KRH39_002597"
     CDS             76..792
                     /locus_tag="KRH39_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBG8512489.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            871..2181
                     /locus_tag="KRH39_002598"
     CDS             871..2181
                     /locus_tag="KRH39_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBG8512490.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            2281..2817
                     /gene="lepB"
                     /locus_tag="KRH39_002599"
     CDS             2281..2817
                     /gene="lepB"
                     /locus_tag="KRH39_002599"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG8512491.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            2983..3519
                     /gene="lepB"
                     /locus_tag="KRH39_002600"
     CDS             2983..3519
                     /gene="lepB"
                     /locus_tag="KRH39_002600"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG8512492.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            3529..4284
                     /locus_tag="KRH39_002601"
     CDS             3529..4284
                     /locus_tag="KRH39_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBG8512493.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            4358..6025
                     /locus_tag="KRH39_002602"
     CDS             4358..6025
                     /locus_tag="KRH39_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBG8512494.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            6566..7495
                     /gene="glsA"
                     /locus_tag="KRH39_002603"
     CDS             6566..7495
                     /gene="glsA"
                     /locus_tag="KRH39_002603"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBG8512495.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            7593..8156
                     /locus_tag="KRH39_002604"
     CDS             7593..8156
                     /locus_tag="KRH39_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8512496.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            8644..9480
                     /locus_tag="KRH39_002605"
     CDS             8644..9480
                     /locus_tag="KRH39_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBG8512497.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKKELKMIKEILS
                     SK"
     gene            9764..10759
                     /locus_tag="KRH39_002606"
     CDS             9764..10759
                     /locus_tag="KRH39_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG8512498.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(11016..11420)
                     /locus_tag="KRH39_002607"
     CDS             complement(11016..11420)
                     /locus_tag="KRH39_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG8512499.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            12503..13417
                     /gene="queG"
                     /locus_tag="KRH39_002608"
     CDS             12503..13417
                     /gene="queG"
                     /locus_tag="KRH39_002608"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBG8512500.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQRKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLNYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            13523..15760
                     /locus_tag="KRH39_002609"
     CDS             13523..15760
                     /locus_tag="KRH39_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8512501.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYSLKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
BASE COUNT         6420 a         1457 c         3022 g         5110 t
ORIGIN      
        1 aaacacgaat aagttaaaaa taagtagatg atataaatca taaataaatc aaaaattttg
       61 aaaggaaaat aatctatgaa gctagattta tttttattag caatagttcc tatattgata
      121 gggatgtttt ggataaggtc taaagataga tattgtagag aacccctaat ccatctaatt
      181 aaattttttt taataggtgc ttttctaagt gttataatta tacttttaga aaatttactg
      241 atgaagttta atgtatttga agggtattca gaactaatat atgtatcatt tgtagttgca
      301 ggacttgtag aagaaggtgt aaaagctctg atactgattc ctgccctaat taaagaaaaa
      361 cactttactg aaaagttaga tggtattatt tactctgtat ttttagcttt aggatttgca
      421 acaattgaaa atatggttta tattttttct gaaagtagaa atctagcact gcaagttggt
      481 attaataggg cagttatctc tataccagct catgtaatgt ttgcaattac tatgggctac
      541 tatatttcta agtataaatt tgagggaaat aaaaataaaa gacgtgagta tttgtttatg
      601 gcagtgttaa ttccaatttt attacatgga gtttttgatt tcatattgat gatagaatat
      661 agatgggcta ttatattact tatagtttat gtaataattt tgtggaagat aaatcttgat
      721 aaacttgaga aatatatgaa tcactctaaa aaagtgtttt tcggtaatct taggaaaaag
      781 aagaagaaat aaaatttaaa ggaaagacat aaataattaa agtataataa atgacacaca
      841 agtaaagcag attgaagggg gattatagat ttgacagaat taccaaagga atttttagaa
      901 gatattgaag aaatactaaa agatgaatat gatgatttta taaaaagtta tgaggaaagt
      961 aagactactg ggcttagaat aaatacttta aaaattgaca agaaaaaatt atttgatttg
     1021 gagttgtttg atttgaatca aataccatgg gcagaagaag gtttttatta tgatgataaa
     1081 attgacagac ctggtagaaa tccactgcat gaggctggag catactattt gcaagagcct
     1141 tctgcaatga gtgtaatacc aaaggcagat attaaagagg gtgaaaaagt tctcgatatg
     1201 tgtgctgctc ctggtggaaa atctacatat atactatcta agctaaacga tacaggttta
     1261 ttggtttcta atgaaattaa tgctagtaga attaaggctc taggggaaaa tttagagaga
     1321 tttggagcga gaaattgtat tataacaaac accgattcta atagcttaag aaagacgttt
     1381 aatgggtatt ttgataaggt tattatagat gcaccttgtt ctgggcaagg tatgtttagg
     1441 aaggatgagg ttgccatatc agattggagt tatgcaaagg tagtagaatg tcaatccatc
     1501 caaaaagaga ttataagaga tggatataaa atgttaaaga aggatggcat tttaatttac
     1561 tcaacttgta ctttttcaag ggaggaaaat gagcatgtta taaatgagtt tatcagtgaa
     1621 tatccacatg cagaacttat tgaaatggaa agattatggc ctcataagat aaagggagaa
     1681 ggtcattttg ttgccaaaat aaaaaaatta gaagaagaag aatgtaatgt caaagaatta
     1741 aaaataaaaa atttagataa agaaataaag gattataggg agtttgaaaa gaaattttta
     1801 aaggttgatt tttatagtaa taaaaaaaat aaattttatt taagaggaga aaatttgtac
     1861 ctacttcctg aaatatatcc agatacgaaa aaactaaaag tattgagata tggtttgcat
     1921 ttaggggttt taaagaagaa tagatttgag ccttctcatg cactatctca ttacttaaaa
     1981 gttgaagatg taaaaaatgt tgaaaacttc agtgtacaga gtgaatcaat tcttaaatat
     2041 ttaaaagggg atgttgtaaa ttctaatgat agtagaggat gggttttggt ttcggttgaa
     2101 ggaatacctt taggttgggg aaaagaatct tctggtgtaa ttaagaatca ttatcctaaa
     2161 ggtcttagaa aagtatttta aactattaga aaaaagaagt ataatattta tattgtaggt
     2221 ttatggtaga ttattttgct gtagattgac ttaatattta acacagagga gacgatttta
     2281 gtgggtgaag cagttaaaaa agaagttgta gaatggataa aagtgattgt catagctctt
     2341 gttttggcat ttgcaataac tcgttttata gtgccaacaa tagtcaaagg agaatcaatg
     2401 tatcctacat tagttgaacg tgattatttg atagttaaca gaattgcgta caaggtagga
     2461 gagccaaaat acaaagatat aatagtattc aaaaccgact taacagagga aaatggaaag
     2521 aaaaaagatt tagtaaaaag agttatcggg gttcctggtg accatgtaaa aatacaagac
     2581 tccaaggtat atgtaaatga taagttgtta gatgagactt cctatataca taataatcgt
     2641 actgatggag atattgatat cgtagttcca gaaggaaaat tatttgcaat gggagataat
     2701 agagaaaaaa gtttagatag tagatacgat gaggttggat tggtcgacga gcataccatt
     2761 ttaggaaagg ttctagtcag attgtatcca ttttctaaga taggaactat tgactaaaca
     2821 tataaaattt ggagcaatcg ctgattagct gaaatcacca gaccatacac aatagttgtc
     2881 tatataaaaa agacaacacc ttatttagta gacttatcta aaagatagtg tttaagagac
     2941 attatcaata aagataatat aactataaag gagaatttgt gaatgaatga aactattaaa
     3001 gaagagattg tagagtggat aaaaataatt attactgcac ttttttttgc atttattata
     3061 actcgtttta taaaaccaac attagtaaat ggagaatcaa tgtacccaac acttaaatca
     3121 catgattatt tggtagcaaa caggatgaca tataagttat cagaaccaaa atgtggagat
     3181 ataatgatat ttaagactga tttattacaa gagaatggaa ggaaaaaaga gcttgtaaaa
     3241 agggttatag gtgttcctgg tgaccatcta aaaattaagg attctaaggt ttatataaat
     3301 ggtaagttat taaatgaagt ttcatatata catgataatt atactgaagg cgatattgat
     3361 atggtgattc ctaagggaaa agtatttgcg atgggagaca atagagaagt tagtttagac
     3421 agtagatata aagaagtggg attagtagat gaagaaaata ttaaaggaaa agttatttta
     3481 agagtatttc cttttacaga tataggtatt tttgagtagg tgatttgtat ggcagttggt
     3541 atagcgtcct tattgtttaa tattgaggag gctttaaaca tttgtgagag tataaagcaa
     3601 ataactcatt tagagattgg aattgacaat atatctgaat gtagtgagtt atgtaaatat
     3661 aaagagagaa tttctaagtt aggattatct atagggatac acttgcctat ggaattaaat
     3721 acttgcgaaa acatagagta tatacgaaat tcatggataa attttattga gaagatagaa
     3781 tttgaactaa aaggatttga tttaaggtat tttaaccttc atttgggata tgttatgaca
     3841 aatagagtta gaaagaatag agacaaatat ttaggaaata gtgtggattt tttagataaa
     3901 ttaaatacaa attcatatgt atgtatagag aatacttatt caaaaggtgg ggatttttca
     3961 aatattggaa atatatctta tgattttgaa tatatattta agaggattaa aaattctaag
     4021 atttgttttt gttacgatac tggacactgt ttaattgatg aggatgctta tgtaaagaac
     4081 ttaaaggata aaataagact gatacactta agtgataatg atggtatcaa tgatacacat
     4141 gttggtatag gaagaggaat tttatctgaa gaggggatta aagaggtctt aacattagat
     4201 gcagaatatt tagtattaga aataaattac gaagacattg aagatactat aagtaaattg
     4261 aataatattg taggagaggg ttaaatacct ctctttatct tgttgatatg cctcattaat
     4321 taaaatttat attcatcaaa aggaaggaga tgttgaagtg aaaaaaataa acttaaaaat
     4381 agaaggaaaa gataaggaat attcattaga agaaaattcc cctggaatca gattgggaga
     4441 tatagcaaaa gaattttgtg atgagcataa gggttatata acacttgcag ttgtagacaa
     4501 taaactaaaa gagctaaatt gtagagttaa gaaggattgt gagataaatt ttttagatac
     4561 tactaatgaa gatggagaga gagtttactt tagagtcatg tcatttatat ttgtaatggc
     4621 atgtagagaa atcttttggg atagcagagt tacaatagaa cattcactat cagatgggct
     4681 atattgtgaa gttcatatag atagaaagct taaagaagca gatgttgaaa atataaaaaa
     4741 caagatgaaa gagatagtaa acaacgatta tgttatagaa aaaattgaag ttacaagaaa
     4801 tgaagctatt tctatctttg aaaataatga aatgtatgaa aaggcagagc ttttgaaata
     4861 caaagagtat gatagtgcta aagtatataa gtgtagaaac tatatagacc atttttatgg
     4921 ttatatgttg ccatcaactg gttatataaa atcatttgat attaagcttt ataatggagg
     4981 cgttataatt ttaggaccta gtgaagaaga taagactctt cctatgaaat ttgtacctca
     5041 acctaagcta tcaagtgtat attatgaagc agaagaatgg tcaagactta tgggaattga
     5101 caaggtagta tctcttaata aaataataga aaacaatgaa tatggagata taataaggac
     5161 atttgaagca ttgcatgaaa agaagttatc ccaaatagcg gatatgataa aggatggcaa
     5221 taagagagta gttttaatag cagctccatc ttcatctgga aaaacaagtt ttgctcacag
     5281 attatcaata catcttagag taaataattt aaatcctatt tcaatttctt tagatgatta
     5341 ttttatagat agaaaacata ctcctttaga tgaatatgga aattatgatt ttgaatcaat
     5401 ttatgcaata gatttagaga agtttaactt agacttaaag aaacttttag ctggtgaaga
     5461 aattgatgat ataagattca attttaaaga aggtaagaga gaatacactg gtaaaaagat
     5521 aaagatagat tcaaatcagc caattatatt agaaggtata catgggctaa atcctatttt
     5581 aacatcatct ataccagatg aagataaatt taagatatac ataagtgccc ttactcaaat
     5641 aaatttggat gaccataatc gaatccctac tgctgatttg agaatgataa gaagaattgt
     5701 tagagactat aattttagag gatatagtgc tgataatact attttacaat gggcatcagt
     5761 tagaagagga gaaaagaaga atatattccc gtaccaagag gaggcagatg ctatatttaa
     5821 ctctgcttgt gtatatgaat tggcagtttt aaaacgattt gcgaaacctt tgttggaaga
     5881 aataaaggaa gataatcctg cacatattga agctactaga ttactaaagt ttttgcagta
     5941 ttttgttgaa ttggacgata cttctgacat accaggtatt tcaattctta gggaatttat
     6001 tggaggaagt aaaatagttg attaatgtgt gtattataga ttgttttatg aagattaaat
     6061 ggcatagcaa gtagtgttat tggtttatgt taagtatatt aagtgtaaag tggagaatta
     6121 aattgataca gattttaaag tttgagttga tatataaaaa ggcttgtttt atacttttgt
     6181 atgaagcaag ctttttattt taaattttac ttttttgttt aatattttgt tagtttatgt
     6241 gattattttt taaaagagta aaagagtaaa agagtaaaag agtaaaagag taaaagagta
     6301 aaagagtaaa aggtgttggg ttagaaattt ttggtgttat aaaaatttaa attactggat
     6361 tattagattg ctggattgct aaattactag gttgttaaat tattgaatta ttaaattatt
     6421 aagccattta aactatttaa attatttgaa aaatgaattg attcttgtag aaaataattt
     6481 taaagtataa ttttttttaa cattgtttta ttatagtata aacttgtgct aaaatagaat
     6541 atataacata atataagagg tgcatatgaa tacgttagat gagacattat taaaagaaat
     6601 tataagttct aataagaagt atactaatta tggacaagtc gctagttaca taccagaatt
     6661 aaaaaacgct agaagaaatg atttaggaat ttgtatcata gatagcgaaa ataatcttta
     6721 tagtgctgga aactgtagca caaagtttac tatacaaagt atttcaaaac caattgtgct
     6781 tgctatggca cttatggata atgattggga agacgttttt tcaaacgttg gaatggagcc
     6841 aagtggggac ccatttaatt caataatgaa acttgaaatt aatgacacaa aaaaaccctg
     6901 taatccaatg ataaatgcag gggctatagt aaccacttcc cttataaatg ggagttgttt
     6961 agaagaaaaa gaagaaagaa tgcttagttt ctttagaaag ttagcaaaaa atgataatat
     7021 aggaataaac tatgatgtct ataagtctga aaaaatgact ggagatagaa acagagctat
     7081 ggcttactta ttgaaaagtg atggatttat cagagggaat gtagaagatg ttctagacct
     7141 ttattttaaa caatgctcaa ttgagataga ttctgtagat ttagcgagaa ttggaataaa
     7201 tcttgcaaat tatggagtag atatagaaaa tggtgagcat ttaatgagtg aaatggtttc
     7261 taggattgtc aagacattta tgatgacttg tggaatgtat gatgcttctg gggaatttgc
     7321 tattaaagtt ggcattcctg caaaatctgg agttgggggt ggtataatgg cttctgtgcc
     7381 gggacgcatg gggattggtg tttatggacc tgcgcttgac aaaaaaggta acagcgtcgc
     7441 aggagttaaa gttttagagg agttgtcaaa taaactcaaa ttaaatattt tttaaaatat
     7501 taagaaagga tttttttgac aacaacatat aatttaatat ataattaaat tgtaaagaaa
     7561 tatgaattaa ataaattttt taggaggggt caatgaaaga ggtattggta ttacgagatt
     7621 tggagtgcat aaaggctatt gcgcatccac gtagaataga tattctaaaa gcatttgaca
     7681 aatcgccatt gtcagcaaaa caattatctc aattattgga agaacctcat gcaaaaataa
     7741 actaccatat aaaaatgtta tacaaagttg gtattttaga attagttgag gaaaaaataa
     7801 agtcggggat tgtggaaaag tactattatc caagtgcaaa aaatgtggtt atagggaata
     7861 ggatacttaa tttttcttta gacaatgggg aagaaaaaga agaattgtac atatcaaaat
     7921 ttgaaaatat gagtgaagtt ttttataagg cagcagagga agatgtttta gaaaatgaaa
     7981 atatagttga ttatcatgat atttctctta ctcatgatga attagttgaa cttagtgaca
     8041 ctatgaagtc aaagatagat gaaattttaa ataaaagaca acacaatgtt gaaggttcta
     8101 agtatgatat agctatggtt atagttccta ctcttgagga ggaatgtcct agttagagaa
     8161 ttaattatgg attaattaaa gatataatat tagagattga acatcaaaat ttacttaata
     8221 atcaaaaaat agtttttggt atgggtaaaa caatgatttt taatctctat ttttttgttt
     8281 ttttatacct attgtatttg taaataatat gtatagtgta agatgtatac actatctaag
     8341 aagtgcttag ataatataca aagtatgtaa gattataata tttgaaattg attaaaatgg
     8401 gtataattaa agtgtaaata ttaaaattaa aaattaatat aaattaaatc aatgctagaa
     8461 atatgttaaa agaggaatga gattagttgg gatagaacat cccttagagc ttatgaattt
     8521 ttagtgaaaa agtactatca aataggaatc tccatcttca tatgttggct aaatgtagtt
     8581 aaggtggagt agttcaaaat taaaattgag tacaagtagt agtacaaata aaaggagatt
     8641 aatatgatta aattaggaac acatctttca atagcaaaag gatttgctaa tgcggctgag
     8701 acagctgtat atattggtgc aaatacattt caatttttta gtagaaatcc aagaggagga
     8761 aatgctaaag aatttgatga aaaagatata attaaatttc aagaaattag aaaagaaaat
     8821 aattttgcac cactgcttgc acatgcacca tatactatga atctgggtgg tacaaaggat
     8881 gatgtatacg aatttgcaac aagggtgata gatgaggata taagaaggat ggactcatta
     8941 ggaattgaat acatgtgttt tcatcctgga agtcatgtag gtggtggtgt agattttggc
     9001 atagatagaa tagtaaaagg attgaacaat gccataaagg gtgatgaaaa tataactata
     9061 cttcttgaaa ctatgtcggg aaagggaact gaaataggtt ttaaatttga gcaattaaaa
     9121 agtataatag acaaaattga gcataaagaa aaaataggtg tgtgtcttga tacatgtcat
     9181 attttttcag ctggttatga tatagtgaat gatttagatg gggttttgga agaatttgat
     9241 aaggttatag gcatagaaag attgaaaact gtccatctaa atgatagtat gatgccattt
     9301 ggagataaaa aagacagaca tgctcctata ggggaaggaa agataggatt aaaagcatta
     9361 attgatttta tggaacatcc tagtctaaaa catttaccat ttttcttaga aactcctttt
     9421 gatgatgaag gtcacaagaa agagcttaaa atgataaaag aaatactatc tagtaaataa
     9481 acctttatta aaaatatgtg aaatacttca ccctcaacat aattagggtg gagatacaat
     9541 catgaaatgt gataatttaa attaatggat aggaataaca aaaattgtat acaaattaac
     9601 tgggtataaa gtatgattat attatgaata gactgatata aaatataatt atactatgaa
     9661 tagtatgtaa atatattgca aagattctca taatttgttg tataattaaa gatgacatat
     9721 ttatattata taaaaaatta ttaaaagtta ggtggggaaa gatatgagaa atttaggcaa
     9781 tactaacatg aaaataaaga gagttggctt tgggggcatt ccaatacaaa ggattactca
     9841 agatgatact aacttagtta taaatgagtt agaaaaacaa ggaataaact ttattgatag
     9901 tgcaagaggg tatacaataa gtgaggaagc aatcggaatt gccattgaag gaaaaagaga
     9961 taaatttttc ttagctacta aaagtatgtc cagagattat gattcaatga agagagatgt
    10021 tgaaataagt ttaaataatt ttaaaactga ttttatagat ttatatcaat tccataatgt
    10081 taaagaagag gagtatgata atctttttaa agataaaatg gcttatagtg cccttttaga
    10141 ggcaaaggag caagggaaga taaaacatat tggtataact agtcacaatt taaatactat
    10201 agaaaaagct atagaagatg gaaaatttga cactatacaa ttcccttaca atatagttga
    10261 aggacaagca gatgaagtgt ttaaaaaagc tcatgaaaaa ggaattggga taatagtaat
    10321 gaaaccttta gctggaggtg ctttagacaa tgcaacactg gctattaaat atatactatc
    10381 taaagattac atagatgtag ttataccagg tatggagagt gttgaacagg ttagacaaaa
    10441 tgtcgctgta cttgaaaatc ttgtgctaga tgaaaaagat aataaggaaa ttgaagaaat
    10501 aagaaattct ttaggtaaaa aattctgtag aagatgcgaa tattgtatgc cttgtgctgt
    10561 aggaataaat ataccattga gttttttatg tgaaggttat tatactagat atggattaaa
    10621 agagtgggct aaagaaaaat atgaggttat ggatgttaaa cctactgagt gtattgactg
    10681 tgggctttgc gagagtagat gtccatatga acttcctata agagaaatgc ttaagactgt
    10741 tgttgaaaag ctaggataga ttttatttat tgaaatcggt gtattaaatt tactccgatt
    10801 tctttttatt agatgtggtc ttatatacaa atattaaatt tacatagtta tagctattat
    10861 ataaatttaa tatttgttta atacttatta aatatttgct taatgctttt taaaaacatt
    10921 tgtttgagta tagacataac acaattatta ttgttgtatt agtatagttt caaatactgg
    10981 agtatcaata taatgtcgaa taacacataa aatctttatt tttcaaaaat attataggat
    11041 atatcgtatt tagatactgg ataaagttca atttcaaccc tagggttatc tttatctata
    11101 aaatcaaata gaagtactgg cttaagttgt ttatcatttt ttattattcc acttttttca
    11161 ataccatcac aaatactttt tggataatta tgtaaatccc ccattctctt atttgggaag
    11221 aataatttca agactgttat tagattttct tcaacagttt ccccttgata ctgttggttt
    11281 ataaaacctg caatcatgtt ttcgtatgca agatacttag aatattttcc ctttgaaggc
    11341 atccatgctt gaccatttat gttatgaagc ttaaaattag atttactaat aggtctacct
    11401 ggaattgtta ttttaatcaa atttttcacc atccttatac agtatttatt ataatctgta
    11461 tatgatttaa aagcaaattg ttatttaaag tgtttttaca gagcttgttt gtaaaatttt
    11521 ccactttaga tgtagtaata taagataagt attttgctta caaattttaa aatttgatat
    11581 atgatgggca tattcatatt agactattat aagaccttca taatattaat aagtataagt
    11641 ttagtaaaga gagtaaaaag aaatccatat aagtcaagta aagacttaat aaatggcaat
    11701 gtaaatggga tattaattat aagaaaaaat caatcttttt gcattaacta taggaagaat
    11761 gtcaaaatat aactaatgat atttgaagtt ttagagttta tattaaaagg taagaaggtt
    11821 aagtgggtaa aaaaggttga atagattaaa aaggctaagt atctaaaaga gttaagtagg
    11881 taaaaaagtt aaataagtaa atgaaaaagg ctaagtatct aaaagagtta ataggtaaaa
    11941 aagttaaata agtaaatgaa aaaggctaag tatataaagg agttagtaga taaaaaaatt
    12001 aaatagataa aaagattaag tatctaaaag cgttagtaga taaaaaaatt aaataaataa
    12061 aaaagactaa gtattcaaaa gagttagtag gtaaaataat taaatagata aaaagactaa
    12121 ttatttaaag gagttaagta gataaaaaag ttaaataagt aaatgaaaaa gacgaagtat
    12181 ctaaaagagt tagtaaataa aaaagatgaa gtatctaaaa gagttagtag gtaaaaaact
    12241 aaataggtaa aaagatgaag tatctaaaag agttagtagg taaaaaatta aatagataaa
    12301 aagacgaagt acctaaaaga gttagtaggt aaaaaaatta aatagataaa aagacgaagt
    12361 atctaaaaga gttaagcagg taagaaaatt gaatagctaa aaagattaag tatttaaaag
    12421 agttagtagc tagaaaaggt taaaaagact aaaaaaatta aataaattta aaaagtcaat
    12481 tagattaaaa aggaataatt ttatggataa taaggaatta aaagcatttt gtaatttaat
    12541 aggattaaac tgtgtaggag tagcaggggt agataagtat gataatttag aaaagatact
    12601 taaggacaga caacgaaaag gttatttaac tggtatggaa gaaccaataa ttgagaacag
    12661 aattaaccca agacatataa tgaaggatgc taactctata atagtctgtg catttccata
    12721 tcatataaat agacaagaaa agaataaaaa ttcaaattta tcaaaatatt gtcatggaaa
    12781 ggattatcac attgtagtaa aggacttttt acaacaaatt tgtgattata tttctaaaac
    12841 agtaacagat tttaaataca agttatttgc tgataatgga ccattagttg acaggtattt
    12901 agcttattta tcaggaatag gatattttgg aataaataat aatatcataa ctgatgaata
    12961 tggttcttac gtatttatag ggtatatagt aaataattat aaatttgaag aagataaacc
    13021 actagaaaaa agttgtttta aatgtggaaa gtgtgtaaaa tattgtccgg gaaatgctat
    13081 attgggaaat tatgatatga accccaaaaa atgtttatca tatataactc aaaaaaaagg
    13141 agatttgtca gagaaagaaa aagttgtttt aaagaatggc aaaaaggtat ttggatgcga
    13201 tatttgtcaa gaagtatgtc cacataatgt tgaaatacca acaactcata ttttggaatt
    13261 taaagaaaat ataatagatt atttgaatta tgatgagata caaaatattt caaataaaga
    13321 atttaaaagg agatatggag atagagcttt tagctggaga ggaagaaata taataaagag
    13381 aaatatggaa ataattttag ataagtatga tgactaataa taagagtctg tttcatatac
    13441 tttaatgtgt atttacttta acacttgttg tgtaaaagtc atattatatc attaaagtta
    13501 aatatgggga ggtgggggaa tattgaaata taataataca tctgaaataa atccactaga
    13561 ttatatattg ggtcaagaaa gagctgtaga agctatggaa ttagggttga aaataaataa
    13621 tccagcgtac aacatatata tagctggtga acctggaact ggtaaaagta cttattcctt
    13681 aaaggtttta aatgagtatg catccaaaaa aaatacgcat aaagattggt gttatatata
    13741 taattttgaa aaccctagag agcctattat agttgaatta gaaaaggggt ttggaagaga
    13801 acttaagaaa gatatggaga aattaattga aagtctttta gaagatttta aaaatgcgtt
    13861 tgaaagtgaa aattttgaaa tagaaaaaaa taaacttctg gatgaatatg aaatagaaaa
    13921 agatatgctt ttgaagcaaa ttaaaaaata tggagaagaa aaaggattta aattaaaaca
    13981 gagtaaaatg ggaatagttt ttatcccttt gaaagaagag gaagatgaaa gtgatgagtc
    14041 tgatgaagag ttttacaaag ctaaaagaga actagaaaat atggcgatac aggtcgtata
    14101 taaaattaga aacttagaag aaatagctga aaaagcagtg ttagagcttg aagaagagat
    14161 agctaaactt gtagtagaac ctcatatcaa gagattgtgt gaaaaatatg aaaattatga
    14221 taagattcaa acttatctgg aaaatataaa gaaagatata atagaatata tgtatttatt
    14281 ctatttagat gaagaggagt taaaagataa gtatgataaa gagcatttta ttaaatataa
    14341 aattaaccta tttgtagaca atggaagctc aaagaataaa gaatcagcac ctgttgtagt
    14401 agagataaac ccaagtccag caaatctatt tggaaaggca gagtatgatt atgcaaatgg
    14461 aaatataaag actgatttca caaaactatt atcaggagca tttcataggg ctaatggtgg
    14521 ttatttagta ctttatgcag accaattact aaaatataca atgtcttggg aaattcttaa
    14581 aaagactttg cagactaaaa aagtaatatt ggaaacacaa acagcaatta aaccagaaaa
    14641 tatgccattg gatgttaaat tagtactgat tggaagccac tatatatatg atattttata
    14701 tagatatgat gaagattttg aaaaatattt taaagtattt gtagactttg atagtgaaat
    14761 ggataaaaat gaagataatg aagaaggaat agcaagattt atagcatacc aatgtgataa
    14821 gaataattta aggcatttta ctaaatcagc agttgaagag gttattaaat ttagcactag
    14881 attatctggt gatttagaaa aattatcaac taagtttaat aaaataatgg aagttgtaat
    14941 agaaggaagt gcttatgcag agtttagaaa ttcggagtat acagagcagt gtgatgttaa
    15001 aaaagcaata agtgaaaaga gaaaaagaat caatagagtg gaaacacata tggatgaatc
    15061 aattgaaaat ggatttactc taatagaaac agagggaaga aaagtaggag tgataaatgg
    15121 tttgtcagtc ttaagtacag gagaatactc ttttggtaga gcttcaagga ttacagcgac
    15181 cactagccca ggaagtaaag gcatagtaaa tatagaaaga gaagtaaata tgagtggctc
    15241 tatacataac aaaggagtac ttatattagg tggctatctt tctgagaatt ttgcacagga
    15301 tttactattg tcactaaatg catatgtttg ttttgaacaa aattatggtg gaatagatgg
    15361 agatagtgca tcttctgcag aattatatgt actattatca tctcttagtg gagtatcaat
    15421 taaacaaaat attgctgtta caggttctat aaatcaaaag ggtgatatac aagtagttgg
    15481 tggtataagt gaaaagatag aaggatttta ttctatatgt aaaactaaag ggttaaatgg
    15541 tgagcaaggt gtcataattc ctagaaaaaa tcaaagaaat ctcgttttat cagatgaagt
    15601 taatgatgca gtgcgagatg gaaaatttaa gatatatact gttgaaagag tagaagaggc
    15661 tatagagata ttgacagatg ttaaatttga agaaataaag aaattggtaa aagaaaaatt
    15721 aatatctttc agcaagatac aaactgtgtc aaaagaataa gtttattata gaatttaatg
    15781 ggtatttatg tataataaaa agcaagaact ttaataggtt tttgcttttt ttgtaaatat
    15841 atataaatta aattcttatt ttgtatgttt tatatattga tgtttatttt ttgttgatta
    15901 aaaaatagtt acgcacactc gtgactttag tcatgagtta gtagctaaaa tagtcagcat
    15961 atagggaaac ttatatgtag agataggata gaacctatcc aatactact
//
LOCUS       DAEORZ010000095        15828 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEORZ010000095 DAEORZ010000000
VERSION     DAEORZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.095"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            73..669
                     /locus_tag="KRH39_002610"
     CDS             73..669
                     /locus_tag="KRH39_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBG8512502.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(767..1726)
                     /locus_tag="KRH39_002611"
     CDS             complement(767..1726)
                     /locus_tag="KRH39_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG8512503.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSCSPKGYMLITNNENIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(1760..2491)
                     /locus_tag="KRH39_002612"
     CDS             complement(1760..2491)
                     /locus_tag="KRH39_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512504.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATNGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(2484..3254)
                     /locus_tag="KRH39_002613"
     CDS             complement(2484..3254)
                     /locus_tag="KRH39_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512505.1"
                     /translation="MFEKIHKRFKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKIYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(3339..4049)
                     /locus_tag="KRH39_002614"
     CDS             complement(3339..4049)
                     /locus_tag="KRH39_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG8512506.1"
                     /translation="MFKFDVTKELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(4230..4382)
                     /locus_tag="KRH39_002615"
     CDS             complement(4230..4382)
                     /locus_tag="KRH39_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBG8512507.1"
                     /translation="MATFIIAAIVVVLMALAVMHMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(4408..6564)
                     /gene="feoB"
                     /locus_tag="KRH39_002616"
     CDS             complement(4408..6564)
                     /gene="feoB"
                     /locus_tag="KRH39_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG8512508.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASNNNKLNFKLPFVDKSKEAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKAILNSIEEITRNCEDELDDDSESIITANRYEFISSIISNIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQILLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIIAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIVIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(6664..6885)
                     /locus_tag="KRH39_002617"
     CDS             complement(6664..6885)
                     /locus_tag="KRH39_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG8512509.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(6898..7110)
                     /locus_tag="KRH39_002618"
     CDS             complement(6898..7110)
                     /locus_tag="KRH39_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG8512510.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(7431..10328)
                     /locus_tag="KRH39_002619"
     CDS             complement(7431..10328)
                     /locus_tag="KRH39_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8512511.1"
                     /translation="MKAFKKHFLILSFIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHPYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNIAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKYKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSNLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKANMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSIESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLKKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEVLVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(10656..11264)
                     /locus_tag="KRH39_002620"
     CDS             complement(10656..11264)
                     /locus_tag="KRH39_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBG8512512.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFIAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMNVIPKCPTLLVFAKYDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDIYSNNYSDIQTKVFTIKEKNFLSKCLA"
     gene            complement(11524..12069)
                     /locus_tag="KRH39_002621"
     CDS             complement(11524..12069)
                     /locus_tag="KRH39_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG8512513.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(12187..14010)
                     /locus_tag="KRH39_002622"
     CDS             complement(12187..14010)
                     /locus_tag="KRH39_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512514.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSIILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVDNVFKSELNDNKDGSLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSIDRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANSHKFIVKMREGYSTILSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(14023..15798)
                     /locus_tag="KRH39_002623"
     CDS             complement(14023..15798)
                     /locus_tag="KRH39_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512515.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLNYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIERDESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNISFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRERIGVVFQNSSVFGKSILENVVMGNRDICKEDVIKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         5249 a         2833 c         1555 g         6191 t
ORIGIN      
        1 tgatattgat tatcattcgt agtataatct aattacttga tattaatttt catttgaagg
       61 aggttatctt ttatgtcttg ttgtaattca caattttgtt gtaaaaataa aattgaatca
      121 tcatatataa agtggctttt agaagtttta ggtccagtct ttttaggctc caaacctgct
      181 gaaataataa atatttcgtt tactgatgta aatagagagg aaaaaattaa tgatatttat
      241 aaatatctat ataagtgtaa gaaaatagat tttatagtta ttgataaaga aaaaaaggga
      301 ttaaaaatct tatttgtaaa taaaaaagca ttatcagaaa aattaaaatg taaaaaaaca
      361 gtaaactttt taaagttttt aggatacaaa caaaatacga atgttaatgc ttatttagag
      421 catttagtag ataaacttaa gagtgatgtt tttcctgatg aaataggtat ttttttagga
      481 tatcctctta aagatgtgat tggatttatg ggatatagta attatgaagt ttctatgatt
      541 aaatattgga aagtttatgg tgacacaaaa caatccgagg acacatattc gaagttttta
      601 cttcaccgta agaaaatgag aaaattacta gactacataa gtgtagataa aatagtaagc
      661 tgtttttaat tgtgtttttg tataataata aaaatacacc ctcttttacc atacagtttc
      721 cataataaat aagggtgtat tttccacttt aatattttaa agtattctaa ttttgattgt
      781 acaacatatc tttaatattt attttctttt gttgcattcc attagacatc aaaaattcct
      841 gtgtatcttc taattctttt atatccttgt ccgttatttt agaactaaag tcataccaag
      901 aatacatttc ttttgtttca tctaaagata aaccaacttc tttagataca acatccatca
      961 cttcatcttt gttttcattc atatatttta aagtttcttc atgaaccttc ataaatcttt
     1021 ttactaattc aggatatttt tcagcaaaat catcacttac agcagtaact attattccac
     1081 ttactaatcc ttctccattt acaatcattt tggcacctga atttattgtt ttcaatgcca
     1141 ctggacctgc aagcattgct acatcagcac ttttactttc taatgcagaa gcagcactag
     1201 gaatatccat atttacatat tcaacatcat ctatagtata gccttcttta gcaagtgcag
     1261 caatcaacag ttgatgaagt atagtccctt ttggacctac aattttttta ccttttaagt
     1321 cttttataga tttaatattt tcattattag ttataagcat atatcctttt ggtgaacaac
     1381 tataaatatt cgtaatttta agaccaacac catttgatgc agctattatt gcagacgttc
     1441 ctcctaatgc atgtaaaaag tctagctcac ctgctgctaa tgcttgagtt tgctctggtc
     1501 ctgttgtcaa ttcatgaaag ttaactttta tattgtcctt tttaaattcc tttccaaata
     1561 aatcatcctt tttttctaat atactaggaa cattaagtgg tgattttaca taagttaaat
     1621 ttatttcctt aggtaaacta gcctcttttt ttgcaggttc atctttctta tcatttgttg
     1681 ttgaacatcc tacagttcca accaacacca ttattaaaga tgtcattact gctaaaatct
     1741 ttttgtttct tttaatcaat taaaactccc ccaattccct taatatttct tttttcaagt
     1801 ttatataata gtttttagtt aaatccctat tatattcatc ttcgacacta aactgtttaa
     1861 tccttttatt tttactaaaa acaattattt tctttgctat ttccatagct tcttcaatat
     1921 tgtgagttac aaatactaca ccttttttag tatttttatg gatatttaca acttcttttt
     1981 gcatttttct tcttgtgaag taatctagtg ctgcaaatgg ttcgtccatc agcaaaatat
     2041 cgggattgaa ggaaagcgct cttgctatac ttactctgtg agccattcct cctgataatt
     2101 cgtttggata tgagttttta aacttttcaa gcttcatcat ttttaaatac ttgtctaaat
     2161 caactttatt tctattatct ttttcagtat gtaaaagtat attttcactt acattcagcc
     2221 atggcataag cctactttct tgaaatacca ttcctacttt tggagtacat tttttatcat
     2281 tattaaaaaa atatacgttt ccattagtag cattttcaag ccctgctaaa attctaagta
     2341 gcgttgtttt cccacatcca ctttcgccta atataacagt tatctcttca ctagatatat
     2401 ttaaagatat attatcaaga actaaatgct ctttattatc aactaaatat ttcttatata
     2461 tattctctag caaaaatcca cttctaacca tttaaatcaa cctgcttccc tttgctaaat
     2521 tttgttatta tctttgaaaa taaataatct gaaagtatac ctaaaaatcc aataacaaaa
     2581 attcctacca agacaacatc agttctagac atatcttttc catcagaaat caaatagcct
     2641 agtcctgaag aagctgcaat aagctctgct ccaattatag ccctaaaact atacccaagc
     2701 cccaaactaa gtccaacaac aatatccagc atagaattag gaattattat tttataaaaa
     2761 attctctttt tagataaatt aaaagattta cctacttcta ttaatttatt gtcacaattt
     2821 tttatacctt tcaatgtatt taaaaatatt ggaaaaaatg atgccaaaat aataattata
     2881 attttagatt cctcacctat accaaaccat aaaataagca ttggcaccaa agctagtgga
     2941 ggtgtacttc gcataaattc aaatagtggt ttgaaatatt catatatttt tttatttact
     3001 ccaaatacaa ctccaagtgg tatagccatc aatgaactta ttaaaaaacc tattaatatc
     3061 cttcttacac tggcataaac atttaagaaa atagaaccgt catatatcat ttttataaaa
     3121 gtatctaaga ctatttcagg aggaggcaga atatagctac tccatatacc caaaacacat
     3181 actatcttcc ataaaattaa aattattaaa aaaatataat atcctttaaa ccttttatga
     3241 attttctcaa acatataaaa ctccttttaa catatctatc cctgctaccc tttttatttt
     3301 ttaatatctt aaataataat caagctatat cataagattt aatctatgcc aagtcttaat
     3361 ccacctgaac tcattctatc agaaggtgta gaatctgttt ttaagccaga caatctttca
     3421 gatggttgta taatgactat ttgtccaggt tcaacaaatt ccatatgata agattctcct
     3481 gaagtctgac cgataaaagt cttccatgat agttttgata ctttaggata tggttctctt
     3541 ccagtccaag ctactaatgc atctgggtca acacaacaag gtccttcaag aattagagga
     3601 ttaccatctg ttattatagc aacatcttga ttttgacctg ttttatttac taaatgtgta
     3661 gtaaaaagac ctttttgaga tattacccct gaaccaatca attttacact gtaatttaat
     3721 tgttcagaaa atgccaataa gttttcactc tcaacataaa tggaatcacc tgggtcaatt
     3781 gatattacat ctacatggta agcattttga gctaggtaaa tttcaccttc accttcaacc
     3841 accattattg gcatttgttc tcctgtcaat gacctctgaa catgacctaa taatgctctt
     3901 cccaagccac caccattact tggacctagc agcaattttt caaaattaaa atttccttta
     3961 aatgctacca tagcaccttt ttttgcaaaa aatttaccac ttccccttgc tatacacata
     4021 agctcttttg taacatcaaa tttaaacata atattcactc atattgctta atttagcaat
     4081 aatccctcct aatcatatat ttgtctataa ttatacaata aaattacaac atagtctaac
     4141 ttacttcaaa tatgaaattt gttatttata taaaaaataa gaacctgata ataaagcaat
     4201 ttaaactttt tatcaggctc ttatcctatt tatctcttag cgctatgaca actcttacta
     4261 ctcgagcatc cagaacaacc acatccacaa ctgctaccat tcttttttga atttttaacc
     4321 atatgcatta cagctagtgc cattaatact actacaatag ctgctataat aaatgtagcc
     4381 atattcatca ctccttattt atatgaatta atttttcata taacttaatt tacccatttt
     4441 ttcttttttc atatttttat ttgaataagt taaaactaca aagactactg ctactgctat
     4501 aactatagat attattgccc cagcaatact tcctccagta cctaaaacta aacttcctac
     4561 ttgattgata agaagagcaa ttatatatgc tgttaaagtc tggaatccta atgtaatcca
     4621 tgtccatttc catgaaccca tttctctttt tatggctcca atagctgcaa aacaaggtgc
     4681 acaaagcata ttaaatgcca taaatgcaaa cgcactagct actgtaaaca tacttgcaac
     4741 tgaagcaaga agtgttgggt cttgctctgt tgcatctgat ataccaaata aaactccaaa
     4801 tgttccaact acattttcct tagctactag acctgtaact gttgctactg atgattgcca
     4861 atttccaaat ccaagaggag caaatattgg tgcaattata tttcctaagc tagctaatat
     4921 actatctcca gcatctacca tttgtaacga ccaattaaat gattgtaaga accagattac
     4981 tccacaagca acaaatataa ttgttcctgc tttgattata aatgcttttc ctctatccca
     5041 catatgtatt aaaacacctt ttgcacttgg aatatgatat tgaggtaatt ccataacaaa
     5101 tggagatggt tctcctgcaa acaaactagt tttctttaat ataattccac aaatgattat
     5161 cattgcaata cctaagaaat acattgatgg agctacccat gaagcacctc caaataatgc
     5221 ccctgcaaat aatgctatta ttggaatctt agctccacat ggaatgaatg tagttaacat
     5281 aatagtcatt tttctatctc tatcattctc tatagtacga gttgacataa ctcctggaac
     5341 tccacaacct gaacttataa gcattggtat aaatgatttt cctgaaagac caaacttacg
     5401 gaaaattcta tccattataa aagctacacg cgacatgtac ccacaatctt ccaatattga
     5461 taataataag aataaaagca ggatttgtgg tacaaatcct aatactgctc caactccacc
     5521 aattagacca tcaactacaa gcccttgtaa ccactctgct acatttaaag aagctagaaa
     5581 attacttaca ttaccttgaa taatttctcc aaacagtaca tcatttgtcc aatcagttgc
     5641 tatagtaccc aatgaactta ctgctatata gtaaactccc cacattataa gtgcaaaaat
     5701 aggtaatgct agtatacgat ttgttacaat cttatctatc ttatctgata cagtttcctt
     5761 accttttcta ttcttcttta ttatatttga aattattgaa ctaataaatt cataacgatt
     5821 agctgttatt atactttcac tatcatcatc caattcatct tcacaatttc tagttatttc
     5881 ttcaatacta ttcaatattg ctttagaaat atcaagtttt tgtataacat tttcatctct
     5941 ttcaaatagc tttatagcta accagcgtgt ttctacatct atatatggag tcttttcttc
     6001 tattattttt tcaatttttt ctattgcttc cttactctta tcaacgaaag gtaatttgaa
     6061 atttaattta ttgttattac ttgcaagttc aactgccttt tcagcagcct ccataattcc
     6121 ttgaccttta actgctgtta cttctaccac tgggcaacct ataacttcag atagttcttt
     6181 aatatttatc ttatcaccat tcttattgac aatatccatc atattaagag ctataacggt
     6241 tggtatacct agctctaaaa cttgagttgt aagatataaa tttctctcta tatttgatgc
     6301 gtctacaata tttattactg catctggctt atcatctagc ataaaattac gtgtaactac
     6361 ttcttctaat gtatatggag ataaagaata aatacctggt aaatctacta tttccacatc
     6421 tttatttcct tttaattttc cacctttttt ttcaactgtt actcctggcc agttaccaac
     6481 atattgtgat gagcctgtaa gcccattgaa cattgttgtc ttaccacagt taggatttcc
     6541 tatgagacct atttttattg acatttccat tccctccatt attatttaaa tacatcacta
     6601 ttcatgatat agaaaatcat tctcattata taattacatt aactctactc taattaatct
     6661 ttattattcc acaataattt tttctgcatc tgacttacga attgatagtt cataatctct
     6721 gactgttacc tcaattgggt caccaagagg agctactttt cttataaata tattaacgcc
     6781 tcttgtaatc cccatatcca ttatacgtct tcgtgtagct ccttctcctt ctaacttttt
     6841 gacttttaca gtctctccac acttaatatc cttcagtctg ttcatgtcat tacctcccta
     6901 tattattatt ctacttgcca tttttttatc taatgcaact ctagtatctt ttacgtctat
     6961 tattaagtta ccatctatct tagaaactat tttaacattt gtacctacta caaaacctat
     7021 actatttaaa aaagtcctcg tcttatcact tcctgaaatt ttctttataa ctacttcttc
     7081 acctgtattt gatagtatca atggcatcat ataatatccc tcctcttaaa ctaatttaaa
     7141 tctacaagca atcgttatct tatatataaa ttacaatacc ttaaatctta atctattaaa
     7201 ataatatcct attttttcct tctttttaca attcctaatt ttgatttaag tctatcacaa
     7261 tagtatattt tttgcaagtg attattgtat tcttttgatt ttttttctca tttgatttat
     7321 tttattaata aaatcgtaaa aattaataca aaaataaaga tgctctaaaa tagtatactc
     7381 aactaatatt aaagcacctt tattatatat atcattactt acacattgcc ttattctttt
     7441 ttaaaagcaa ttttttcaaa ctcttttaca ggcattggct tagaaaacaa atatccttgt
     7501 accatgtcac acccaatttg ctttagaaat tctacttgcg aaatagtctc tacaccttct
     7561 gatattactt tcattttcag ttcctttgcc attttaatga cattagatat aacaattttt
     7621 tcattattac tctcatcttt ttctgtaaaa aatgctctat ctagttttaa tatgtcaaca
     7681 ggcaattctt ttaaaagatt aagagaggaa tatcccgtac caaaatcatc catagagatt
     7741 acaaatccct tttctttcaa attgttcatt gtatcaagta atccttctat attatctaat
     7801 actgctgttt ctgttagctc taattctata agtcttgtcg gaattttgta tttatctaca
     7861 attaatttaa acttctctat aaaattacta tttacaaaat gcattcgaga caagtttaca
     7921 gatataggta ctactttttg tccttcatcc atccattctc taatcttttt acacacttcc
     7981 tcaaatacat agaaatcaat atttacaata aaaccattcc tctcaaatac aggaataaat
     8041 tctattggtg gtattagacc tttttgtgga ttatcccatc gaacaagaac ctctgcccct
     8101 ataatttgat aatctgaaag actatattta ggttgaaaat aaacaataaa ttctccatta
     8161 ttaagagcat ctaccattga attttcaatt tctttctctt ttaatatttt tttatgcatt
     8221 tccttatcgt aaaaagcaaa tgaattttta tgaccacctt ttatagtttt tcttgcagta
     8281 ttagctctat ccataatagt atttatgtca ttatcacttt tacttatttt atatatacca
     8341 caatctaaaa tcaatttata atagtttatt ttaggattac taagtatttg aaccttattg
     8401 taaatattgt ttaaccttgt tttaatatca tcatctgtct tatacttaat aagaagaaca
     8461 aaatggtcag cagaaactct tgcaaaaatt tcatcttcct ttaattcact tgcaatagta
     8521 tttgatatat gtatcaaagt atcatttccc atatcatatc caaacatatc atttatatat
     8581 ttaaatttgt caacatctat ataaaacaat gcatattctt ctggattatt tttagcaaac
     8641 agtttattcg aatttatttt aaatttatct atactatccg ctcctgtcaa tgggtctgta
     8701 tatgctatct tctttagata atcctctttc ttctttttat tataaattat atatttaata
     8761 gcaattaaga ataaaataca tacaaaagaa ataatttgta ttgaatattc tttaaaaatc
     8821 acaccaaatg gtactttata aggtttgtca atagtattag aatatataca atcacttaat
     8881 tcttcttcag taattttgtc tattgattta tttaaaatag atactaatat agggtcagtt
     8941 ttatttgata cccctatacg catcccatag cttgtatcta aaacagatat aacagataaa
     9001 ttctcactct ttccttctct taaaagttca tcaaactgat aagaactaag tatcattaag
     9061 tttgctttac cttcatttat agcgtttaga caactttcta tactcttata agatgttgtc
     9121 ttagcatttt caaaactttt ttctatataa gaatcaatag acttataact actcgttaaa
     9181 gcaatattta aatcattatt gagattggta tccagttttc ctaccataat cattggaata
     9241 tttatgtatg gatttgtcaa tttcatatta aatcgttttg ccttatcatt ttcattgatt
     9301 gagccacata gcatatttgc cttaccactt tttatgtaat caagactctc attataattt
     9361 tttgttttaa tatattcaaa ttttatacca catttttttg atataattga cattaaattg
     9421 gaacttatac ctttaaacga attgctttcc ttatcataat actctacagg agaccactct
     9481 gaatcatata ccatagtaag ttttgggttt gctttaataa aattcatttc ttgccgagta
     9541 aatgctatgg aattattgac ctctcttttg aaatattttt tatataattc caactcataa
     9601 tacatatcat ttatttttaa ttcttttaaa gcataatcta attcttttac caaattaggt
     9661 ttatattttg cagtagcaaa ataaaaaggc tctactgaaa atttgctaac tattttcatt
     9721 cctttagcat ttataatact tccacagaca aatgcatctg cctttttttc attgacagac
     9781 tcaattaatt catcaataga attacagtag atatattcaa ctgaaaaatt attttcttct
     9841 ctatacttct caaatgcaat attatgaaag tttcctctaa gtacagctac tctcattccc
     9901 ttaaaggaac ttatatcttc ataaaacata ttattgttac tatcttttgt atatagtgca
     9961 ctatattcat atcctgaact cagtttagga aaattaaata gtttatttct ttcatcattc
    10021 ttctgtaaag gtccaagtaa gtctatttca cctttttcta gtctgtctaa acattcttcc
    10081 caagaccctt ttacaaattc atattcccat cttgtatatt ttgagatttc tcttagataa
    10141 tcataaccat atcctgtata aggatgctct tttgatgagg atattattcc gtagtcatca
    10201 caatatccaa ctcttatttt ttttgaattt tcagactcat cagcaaagca tagtatattg
    10261 ttaaaacatg atataataag taaaaatata aaagaaagta tcaagaagtg ctttttaaat
    10321 gctttcatat ttaaaaacct ccaattttcc aacttacttt aacttatttg gttttctagt
    10381 tgtaactact tttattgtaa tggtaaatat atcaaattgc aatattctca tataatattc
    10441 ttattcaact ataatgaatt tattattatc tatttaaata ccctatctaa tattaatttc
    10501 ttataagtca taaaagcata attaacacta taattctagt gattaatttt atttcaaaaa
    10561 atataataac actactgata ttttaaatat caatagtgtc ataaaaattt tttcagatta
    10621 attaaatctt tctatatatt ttataataat ccaaatcatg ctaaacattt agacagaaaa
    10681 tttttttctt taatagtaaa tacttttgtt tgaatgtcag aataattatt tgaatatata
    10741 tctataaaac catgatttgc atctaaaatc tcaatttgga tattttgttt cctagaaagt
    10801 tggtaagaaa tagaatcaac atcaaatgaa tcgtactttg caaatactaa taaggtagga
    10861 cattttggaa ttacattcat gtaatctcga atccgagaac cataacaaca aattatgcca
    10921 tcacaaaaag aatcttcact gcatctccat gcaatagttg ctcccacgct atacccagca
    10981 ataaatacat atttatactc tgattttaaa tcatttataa gcttttcaac ttttttataa
    11041 atatcaaaac caacattatt tataaaaaaa tcatatgcct cttggacttt tgaatatgga
    11101 aaaactcttt tttctcccag aagttcagga caaaatacat catacccata tttataatac
    11161 ttttgacaaa catcttctat aaagctatta atcccataga tttcatgtaa aacaacaata
    11221 gctacatcac ttttatgtat atactttatc agattatcca ccacctttac aaaacagcaa
    11281 attttttata cttagtttaa tatattcttc tttacatgtc aatttacttt tctaaaatca
    11341 aatttatttt tggattaatc atttattttt aaaagttgtg cattattact attaaattat
    11401 tctctattat taaaaatata atcgttagaa ataaaaaata aagtacaaat aattaaaaaa
    11461 tatctcactc ataactttaa aaattataag tgagatactc ctaaaatcta ctttatatta
    11521 aatttatttt ccaaaatatc tatttaataa tcctaagaat gctttaccat gtctagcttc
    11581 atctttacac atttcatgta ctgtatcatg tatagcatct aatcctaatt cttttgcttt
    11641 cttagctaat tttaattttc catcagttgc accatactca gcatcaactc taactcttaa
    11701 gttttctttt gtatctgcaa ctacaacttc tccaagaagt tctgcaaatt tagcagcatg
    11761 ttcagcttct tcaagagcta ttctcttata agcttctcct acttctggat aaccttctct
    11821 atcagcttgt ctactcattg ctaaatacat tcctacttct gtacactcac caacaaagtt
    11881 agctcttaat ccttcgatta tttcttcatc tacaccttga gctactccta ttctgtgctc
    11941 atcagcccaa tttttttcgc ctttcatttc ttcaaattta tcagctccaa ctttacatac
    12001 tggacattgt gctggtgcag catctccttc atgtatatat ccacatactg tacaaacaaa
    12061 tttcttcata attactttcc tcctaaattt tatatatcaa attaaatttt ttatttatta
    12121 agttagtcta tatatacccg caccatatac ctttaaacaa ttttttttaa agttttttct
    12181 aaatacttat tttccctgtg tcaataatat gtttctataa taatgacctt ttaattttat
    12241 gagttcttta tgatttccag attccacaat tctaccattt tccaaaacta ttatattatc
    12301 acaatttgat atggtactta atctatgagc tataattata cttgtctttc cataagttaa
    12361 attatttatt gcctcttgaa ttttagcttc tgttgctata tctatattac ttgtagcctc
    12421 atccaaaata agtattggag cattttttaa tactgctcta gcaatactta ttaattgttt
    12481 ttgaccttca cttaaatctt cacccctatc cgataatata gtactatatc cttcacgcat
    12541 tttaactata aatttatgtg aatttgctat tttactagca tatataactt cttcatcagt
    12601 agcctccaga tttccatacc ttatattttc cataactgta tcattgaata tataaccttc
    12661 ttgtggaact actccaaaac aagttctcaa atagtttcta tcaatagaat atatatcctt
    12721 accatctaaa aatatacttc cttcatctat atgataaaaa tttgtaatta gatttataat
    12781 tgttgttttt ccacttcccg tctctcctat gatggcattt gatgtgccag ctttgatttt
    12841 taggtttata ttgtctaaaa ttttcttttt tgaatcataa gaaaaactta catctttaag
    12901 ctcaatttct ccctttacaa aatgagagtt taaactccca tctttattat cgttaagctc
    12961 acttttaaac acgttatcta cttcttgctc ttcttgttca tctataattt caaaaactct
    13021 ttcacatcca gctatggctg aaagtaatgt attaaataaa tttgccaatt catttaaagg
    13081 tcttgtgaat tgtcttacat aacttaaaaa actagctata acccctatgg ttataatatc
    13141 tttagatgca agaacgcctc ctataataca tatggttgca aatcctatat tactaatcac
    13201 attcataata ggcattaaaa gagaagtgta aatttgtgat ttaagggaaa tatttagaag
    13261 tctatcattg acttctttaa aatcatttac agattttctt tcataattaa aagattttac
    13321 tacatttatg tttgatatca tttcttcaat atgtgcattt agttttccca actcaacttg
    13381 ttgttgctta aaaaaaactc tagttctctt tgcaataaaa ttactcacaa acattactat
    13441 cggtattaat attatactgc aaaaggttaa aattgggctt aatacaaaca tcataataaa
    13501 agtcatcaat atgtttaaga ttcctgtaat taactgtact aatgaatttg atattcctgt
    13561 actgatatta tcaacatcat ttgttactct gctcataata tctccagtag aacttttatc
    13621 aaaaaaggtt attggtagat ttttaaattt taaaaataaa ttacttctca tctctttaac
    13681 tatactttga gataatctag ctgtacatat ccccttagac agtttaactg aaaactcaat
    13741 tatataaaat gaaagtaaaa taattattcc ttttaagaaa atggattttg aataagtatc
    13801 gatatctatt atgtcaacta cctttccaat agtatatggt attacagttg atataattga
    13861 atcaattatt actagcaaaa aaataaatct agtaatcttc ttatcttttt taaagtacat
    13921 aaaaattctt ttaaacgttt ttttaaaatc tttaggtttt tcttttttct ttaatctact
    13981 aaccttttga tttccagtac ttaaattcaa tatacttcct ccttaattta catcttcact
    14041 tactaaattc tgcaacgcaa aaattttttt ataaattgga gaactaacca gtaaatcttc
    14101 atgtgtagca aatccaacca cctctccctc atccaagact aaaatcttat cacagtcaat
    14161 tatagaacta atcttttgtg aaatagttat tacagtacat ttcttacttt ccttttctct
    14221 taaattactt atatattctt ttatattctt tcttataatt gtctctgtat taatatccat
    14281 tgcactaaaa caatcatcaa gaattaatat ttgtggtttt tttgctatag ctctagctat
    14341 agtaattctt tgtctttgtc ctcctgatag attcttaccc ctttgaaata aaacctcatc
    14401 ataagtattt tcgagttttg ttataaattg atttgcattt gctatctcgc atgattttat
    14461 aacatcctct ttgcatatat ctctatttcc cataactaca ttctccaata tgctttttcc
    14521 aaacacacta ctattttgaa atactacacc tattctttcc cttaaacttt cttcagaaat
    14581 actgtatata ctacgtcctc caatcttaat atcgccttta tcaattatat ataatttttg
    14641 aattaaattt actaaagaag tctttcctga accagtttga ccaatcacac caatattttg
    14701 accttcttct attttaaagg atatgttttt taatattttt tcatctgtat ttttgttata
    14761 cgaaaaatct acattttcaa aaattatatc acaatttaca aactttaaat ctttttctcc
    14821 aacatctaaa ctttcatctc tctcaattac ttcttcattc tcatcaacca aaacctcact
    14881 tattctctca taagacgctt ttgccctaac aaaaacatta aaaacatgtg aaatcattat
    14941 aagagatgtt aatattctag tcatatagtt tacataagca actataatcc caattttaat
    15001 ttctcctgaa taaattaaat gccttccaat cagtaatatt attattatag aaacattaat
    15061 gattattgta attgtcggtc taaaaaacga catcatagtt gtagcttttc tactattttt
    15121 aaaaagttca tcatttaact cttcaaaact ttggttctca taatcgtatc tacaaaatag
    15181 tttaactact tttattccac ttaaaaactg tcttagtttt gaatttactt tgtctgtact
    15241 ttcttgtaca tttttaaata atctataccc tatcttcata ttaagcaata ttataataaa
    15301 tataattaaa actgcaatta gaataattat tgacatcttt aaatttaatt ttatagctat
    15361 aattaaactt cctatacaca ttattggtga ctttataaaa actctcatca ttccattaac
    15421 gaattgttgc atttgattga tatcattagt aattcttgtt attaatgaag ctctcccaaa
    15481 tttatctatt tgttttaatg agtatgtatt tatcttttca aatacatcct gtcttaaatc
    15541 gtaagaaaaa tgctgagaaa ctcttgctga gattacactt cttattattg caaatattgc
    15601 acctaaaaaa gttataccta tcattatagc tcctgttgat agtatgtaat ttaagttctt
    15661 atttgaaacc cctatatcta ttatttttgc cattatagtt ggttgagcta aatcacaaaa
    15721 agcttccaat gttaaaaaaa atactgctgt tagaaataat actatatatt tttttatgta
    15781 ttttttaaaa tacctcattc aatcatccct tcatagaatt ttctcatt
//
LOCUS       DAEORZ010000096        15640 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEORZ010000096 DAEORZ010000000
VERSION     DAEORZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15640
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.096"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(6..866)
                     /locus_tag="KRH39_002624"
     CDS             complement(6..866)
                     /locus_tag="KRH39_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBG8512516.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTINPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1183..2511)
                     /locus_tag="KRH39_002625"
     CDS             complement(1183..2511)
                     /locus_tag="KRH39_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG8512517.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2659..2988)
                     /locus_tag="KRH39_002626"
     CDS             complement(2659..2988)
                     /locus_tag="KRH39_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG8512518.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3012..4301)
                     /locus_tag="KRH39_002627"
     CDS             complement(3012..4301)
                     /locus_tag="KRH39_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8512519.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4317..4643)
                     /locus_tag="KRH39_002628"
     CDS             complement(4317..4643)
                     /locus_tag="KRH39_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8512520.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4778..5407)
                     /locus_tag="KRH39_002629"
     CDS             complement(4778..5407)
                     /locus_tag="KRH39_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512521.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5622..8279)
                     /locus_tag="KRH39_002630"
     CDS             complement(5622..8279)
                     /locus_tag="KRH39_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8512522.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8638..9582)
                     /locus_tag="KRH39_002631"
     CDS             complement(8638..9582)
                     /locus_tag="KRH39_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512523.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSDINKYQQNLKVV"
     gene            complement(9575..10600)
                     /locus_tag="KRH39_002632"
     CDS             complement(9575..10600)
                     /locus_tag="KRH39_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512524.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10675..12243)
                     /locus_tag="KRH39_002633"
     CDS             complement(10675..12243)
                     /locus_tag="KRH39_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8512525.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12356..13267)
                     /locus_tag="KRH39_002634"
     CDS             complement(12356..13267)
                     /locus_tag="KRH39_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512526.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13251..14237)
                     /locus_tag="KRH39_002635"
     CDS             complement(13251..14237)
                     /locus_tag="KRH39_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512527.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14597..14998
                     /locus_tag="KRH39_002636"
     CDS             14597..14998
                     /locus_tag="KRH39_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG8512528.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15001..15399
                     /locus_tag="KRH39_002637"
     CDS             15001..15399
                     /locus_tag="KRH39_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBG8512529.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
BASE COUNT         4932 a         2726 c         1728 g         6254 t
ORIGIN      
        1 taatattatt taaatattct tctatatttc tcttcttctt cttcagtaaa cacattaaat
       61 acaactttta aatttttttc aggattttct tttagccata gtctaactct acttacagca
      121 agattagctg cctctttttt tggataacca aatacacctg tagaaattcc acaaaaaaca
      181 atatttttta tttcatcaat ttcttttatt gtatttaaac atgatttata acaatgaagc
      241 agttgctttt cttgctcttt acttggctga ccaccagata caataggtcc aacagtatgt
      301 actacaaatt ttgcaggtaa acaataacct ctagttatct tagcatctcc agtatattct
      361 aaatgccctt gtttttttat tattttgtca caatcttctc taagtctagg acctgcgcat
      421 gaatgtatct cattatccac gcatagatgt aatggttgta aacaccctaa taacttgtta
      481 tttgcagcat ttactattgc atctgctctt aaattcgtta tattacctct ccatatggca
      541 atcccttcct ctacttcttc tatatcattt acatctacta actctctttc ttcacattca
      601 atcaacaata aattatgtaa catattaagt atctctggag caatttcttt tggtggatat
      661 acattcaaaa gtcctctaag tatatctcta ggcttaattt catcatttga taaattgtat
      721 attttagaag gaacttcttt tgaaaaatat gcaattaaag tgtttatatt ttttcttctt
      781 tgttcgtcag ttagaggatt gatagtttta tcaaaatcct caaataaatt tacatcatca
      841 gcataatctc tccacttcat agccatctct ccattcttgt attttagact taaaaataat
      901 tatgtattta tgtacatatt tatttatttc tataaacacc tacataaatc ctgttaaaag
      961 tgtaaatttt ttattataca acttctctgg ttatttctat tgattgatag taaattattg
     1021 tacctaaatt taatccaact aacaagtaac taaacctact ttcctaactg caaaatacac
     1081 tatgtttgcc acttgtatct catttgtaaa taaataaagt ccctacaaat acattaacta
     1141 ggctatgtac taaactgtag gaactttaaa aatatattat tcttaattaa gttttggcca
     1201 ataattttta tttgcttcta ttaaatcatc tagtaattct tttgcaactg ttgcactagg
     1261 aactgtctta gatagcataa gtgcttgcca caatttatta taagacttct caatccaagc
     1321 atcaactact aatttttcaa ctgctaattg ttgttctatt aaaccttttt ggaatgttgg
     1381 tattttccct attgataatg gttcataccc gtcttttcca actatacatg gaatttcaac
     1441 catagcatca tcttgtatat tttctatagc tccattattt tgaactatca ataacatacg
     1501 ctcctgtgta ttaaaagcta gagctgtagc taaatccact ataaactctg catgtattcc
     1561 tatcttaagc ccactgccct tagaatgtcc ttgcttttct atcaatgcac atgaaccaaa
     1621 aacttctttt tctcttccat caataacttc attggcacga gtataattta catcagaatg
     1681 atttacaaca taatctggaa atagatagta ctttaagtaa ctacttggta ttgtagttaa
     1741 atctgttttt actatttctt tagtaaataa attagtttca aaccaactat ctttcttgtc
     1801 atttttcatt ccttcttcac catttataaa accatattct tttatatgtt ctactaattt
     1861 tggtaaaagt tcatttcctt ttttatcttt aattgaagta taccaaccaa agtgatttaa
     1921 tccaaagtat cttatatcca tatgtttacg agatttgagt cctgcaatcc ttgctatatt
     1981 atgttccata cctattggca tatcacaaat atttattacc ctagaattag gtctcaacct
     2041 tctaacagct tccgctatta ttgatgctgg attagaataa tttaacatcc aacaatttgg
     2101 agaatacttt tccatataat ctatgttttc aagaacacct tctatagacc tcattccata
     2161 agctattcca cctggtccac aagtttcttg accaactacc ccatgtttta gaggtatctt
     2221 ttcatctagt tctctcattt caagttttcc aactcttata tgagctaaac aaaaatctac
     2281 atctgtataa gcctctttag ggcatgttgt tgctaaatac tctatattag ggtccttttc
     2341 ctttattagt atttcacaag ctttagctac cttattttgt ctttcttcat cattatcata
     2401 taatttgatt gctcttaatg gtaacctatc taagttatct agcaacatta atatgatttc
     2461 tggggtatat gtacttcctc ctccagctat tactactgaa aatttttcca taataatatc
     2521 tccttttcta tttaaaaatt tactgtattt ataaataaat ttataaatac tagtacttta
     2581 aagtaaaagg gagttcattt attaaatcta ttgatttaat aaactatagc caaacttcaa
     2641 gcactattta caagaaattt aattcttatt aattcttggg tccatcatct caaatatatc
     2701 tacaagttca gatactaaag aatgagcaaa taatgcattt gaataatggt cttcagagtg
     2761 cacaactaaa aaatcagtcc cactatcttc actattcata gcatttgata gtaattctaa
     2821 atgatgcttt gatgctgttt gtattttctc tgaggctgaa tttaaaaggt gttttgcttt
     2881 attgtagtca ccttttttta tttccttaag agacatcatt acatcacttt tagcatctcc
     2941 tgcttctgat attatactaa atactttttc ttccatttca ggtaccatat ccttctcctt
     3001 ctaatgactt gttaaaaatc aaagctatca tcatctaatg ttgtagctaa ttctgcattt
     3061 ttatttaatt cttccttctc cattatctta aagaatggat aatatatcaa ggtcataagt
     3121 attatacttg caaatactaa tacgattgcc ttccaatcta atgtcatcaa aaatgctgca
     3181 acccctggag gtaaaaagcc tggtggttct gcaataggta atcctactaa tcctactttt
     3241 aatgccataa aattaagtgc tccaaccaat acaggtccaa atacaaatgg tacaaacata
     3301 tatgggttca taactattgg tgttccaaat attactggct catttattcc aaatagtgat
     3361 ggtaaaatca caacttttcc catactatta aaactcttac acttacttct tgacataagg
     3421 tacactaatc ccaaagttat tcctgaacca gttacttgca agaaaccata gtaaaatcct
     3481 attgtaaaca catattttgg gtctcctcct gctgctatca gtgttgcatt ttctgccata
     3541 tatgtagctg ctattggcat ccatatactt gaagttattg ctgctccatg aagcccaaag
     3601 aaccataata attgagttat aaatagtatt atagttactg caaaaacatt atctacactg
     3661 cttatagctg gtgctaagaa attcattata atagctggta gtggttgacc tgagacattt
     3721 ttagttattg ctgctattgc tgtaaataac attatacata ctatcattgg agttaaattt
     3781 ctaaaacttt tatttactac actaggtact tcttttggaa atctaattac gagattttta
     3841 tcattcataa acttagtaat atcaactact aaaagtgcta ctaatatact tacaaataaa
     3901 ccttgtgcat ctaaataact tgtatctaat acaccatcta ctacttgtcc acatacaaca
     3961 agttgtgcaa caactgctgt catgcctatt tctacatgat taagattata ttttttagct
     4021 agtgcctgag ctataaaaaa tgctgaatat atagataata aactcatagt aaatttattg
     4081 gggataagta atgtatccat attttcagat aataatgttt ttgcacttcc ttcaggtaac
     4141 atattgttta atgccataaa taacatactt aaagaaccaa caattattat tggggtaatg
     4201 cttatcatac cttcttttat agcttgaaga tgcctttgtt tttcaatctt ttgtgctatt
     4261 ggaagaagaa atcttcttaa attattttga agtgcttcca tttttattcc caccaattat
     4321 ctatttttat ataattcgat tgccgacttc ataacagaag gagcatccat acgaccataa
     4381 tgcttagtat ctattaaacc aactgctatt ttgtaattct tacaagcttc aactacatca
     4441 tcttccttaa acctaatttg tggtgctaca agtactacat ctgtatcatt agcttcaatg
     4501 tatgatttta aattttcaaa tgatgttgcc tctatattta tagttttagt ttttaacttt
     4561 tcatgctcac tacaaaaact tttcatcttt tccacaagta cacctgttga agctccattt
     4621 gcacatacta ataaaatatt cataattacc ctcctacaat atctaacctc ttttattttc
     4681 taatttttta tcagccaatt cttactctaa ttcctatcag tttttacttt aattcttatt
     4741 attttatttt aattcttatt aatatttact ctaattctta gtactgccaa tgattaaact
     4801 tgaaagccgt atctataact tcttcttgct tatatttttc taatattttt aagtatcttt
     4861 ctttataatc attatctact tctttttctg gtattactat actagcttca tttaaaaagt
     4921 ggaatgaatc ccttcccatt tctaatcctc tatatgtatg cttgtcaagt gccacgtcca
     4981 taacttctgg aactttaccc attgcaaatc cttttataat tatatttttt aataaatcac
     5041 ttgatctatc tttctcacat gcacaaagat atcttattgc atgtatgaaa aataaaggtc
     5101 tgtctccatc tgcatatgaa tattcctttc ttacttgatt taaattattt atgattatta
     5161 cagctgatgg atcccccatt cctatatctt caactgatat tgtaagtatc cttctccata
     5221 gcttatcttc taattctggt gatgatatat atagttcata agcaaattca caagcttctt
     5281 ccaccatatt tcttcttata gctttttgaa atgacgatat tatttcgtct gcactaaatc
     5341 cgtttcttgt agtcattctt gcccatgggt catttgcctt tttatctctt aatattttat
     5401 cttgcatatt atttactcct ttttatttaa attttaatta tttatattac tcatttaata
     5461 atataagcaa gtattatgcc agttttttta gacactttaa aacactgtta tttcaatcat
     5521 ttttatcatc aaaacttaga tactttgtct aaatatattt atatgtgtct aatattaata
     5581 ttattttaga ctaaaaaaaa ttaccaaata agtttttata ctcattggta atctttttcg
     5641 attatttctg catattctaa tattaataat atttgatagc actctttttc attaatttct
     5701 atattatatt ttttatctaa ttgagatact atatctttta ttttgcttag actcttgtga
     5761 taatcaagtg ttttttcttt atcaagcttt tcaatttctc tagtatttcc aatcaaccca
     5821 tctaccatga aagctaaatg catcataagt ccaattactt tatcgtattt cttttctata
     5881 ttaaatacta aaaacaattc atcagacaca tattttatat ctttaaatat tctattataa
     5941 ttagagcttt ttaaggatgt ctttaaacct tcaactacat catcataacc tatacttatc
     6001 tttataatat tttttaattt attgatattt tcatccttat ctatatcttt tttgctaata
     6061 tagtcaattc ctctaatatt aattctttgt tcattgatta ctgcaacaat attactactt
     6121 tctttaagtt tcgtaatatt taaacttaaa tctttttctg ttttatatgg gacagatata
     6181 atatctattc catctaagta cctactcatc ttttcttcta atatttttct tgttttatta
     6241 tctacacctt ctgaaacatg tgatgctata ataattgttt tagacagatt ttctttattt
     6301 tgaatttcat tagttccaaa atagtacttt ctcgtgttag tgatagagtt gtatacgtca
     6361 tttaaaggta atcccatatt ggccttctga actgcttcta acacagttaa tgtcgtcacc
     6421 ataggaatta ctcttactcc acactttttt tctttcttta taatttcatc aaaagagttt
     6481 aatgaaccca tatctgtaaa tagtaataca tctttattgt ctatattatt aattaaattt
     6541 ttaaaatcta caataacttc atcatagctt ttatctaact tcatatcgaa agaaatagca
     6601 ctgcactttc caataatatc accaactgct tttaccatac tacttgctgt agtttctcca
     6661 tgcatagcaa ctataatatt aatgttattc actattttat tttgttcatc ttgtttagct
     6721 tgtactatga ataatgtaat atatgctaca gtttctatag ataaaaataa atcaaattca
     6781 ttttcaatta ttccaactat ttttagtgct gttttaaatt cacttctgtt atttcttcta
     6841 atttgattca aatctatgtt gtttgtagag ctacttttat ccaatacgct ttttatataa
     6901 attgccattg acttaaatat tgattcactt atatctattt cttcatcttc tttaataatt
     6961 tgtcttattg attcacacat gcttttaaac ttaacattat taaatagaga agcatctgct
     7021 atattcattt ctacagattt attaagatta gtatttactg cattaataat atttatatca
     7081 ttgtagttaa taacttcttc ttctcctaat gacgtaaata caaaatattc tattccatgt
     7141 gttataactt catctgcttt aatagacttc tctctataat catcactcac ttcatttata
     7201 gatttatcaa aatatttttt ctctatataa actggttctt tattatctct tttatatctt
     7261 aggtatccat ttgcaacagc taattttata tcattttgaa gttgacctac attattttta
     7321 caatcataaa gtaatagtga tttaattatt tctttatgta cttttattga aatagaaata
     7381 ctttttgatt cttccttgaa aaaatttttg actaattcca ttctttcaat cattgtccta
     7441 tctctcagtg atggtatttc tattttcatt ggtattcttc tattgaatgt atctaatagt
     7501 gttgatgtcg gattttctgt agtagcacat attattaata ccttagactc tctttctttc
     7561 gttgcatctc ctagtctttt aaatgttccc ttatctataa aagtaaacaa catttcttga
     7621 ccttctgctg gtagtctatg aatttcatct aaaaatagta tccctccatt tgcaatttcc
     7681 aaaagacctt ttctggaaga ttctgcacct gtataagctc cttgttctac cccaaaaata
     7741 tgtcccataa gtaattgtgc attattagca taatcagcac aattaaatgt tacaaaagga
     7801 gctttatttt ttctaattcc actatctata gaataatcgt acatatatct agcaatcata
     7861 ctctttccta ctcctgtttc tccatagata atagtatgaa gtccatttgt aggatagagc
     7921 attgcgacct tgcatttttc tattataggt cttaaactat gttctttacc tattagtgtt
     7981 tcaaaactat ctttttttat tttgttttct acttttgttc ctttttttat accaagcaac
     8041 tcactttgtg tatcaagcaa ttcattcttg ttaaggttaa gatttgcagg gatataatac
     8101 acaggtcttc cttttatctt cgtaaccata ccctcattga ataattggtt taaatatgta
     8161 cttatattgt ttcttgcaat tcctgtcttt tctgataatt ggttagctgt aatccctttt
     8221 tcattttccc tatatgatga ttctaaaact ttcattactt tatctttttt atatatcatt
     8281 acaagctccc ctacattata atattttatt aactaaaacc cctatacaca ctttattttt
     8341 taataaactc ttattcttta actaaggtta taatttatca aactttcctt actttaaatt
     8401 atagaaaatt attgtagaaa aaataagatt attgctcatt ttttctacat tactatgttt
     8461 aattttatag tactttctta acaacatact ttacttgttt ttaattttat tatatttgaa
     8521 tttattatat ttgaaaagta gaaataaaaa accctatttt ggagtagttt taaaatttct
     8581 ccaaaaatag ggttttagtt tgtatttcaa ataaatacat gttcaagtat ttttatacta
     8641 aacaactttt aaattttgtt ggtatttatt tatgtctgat ttagaagaat atacaaaatg
     8701 tccatctgaa acctcaatcc aacttaatga cttttcattt gaacaatcat atccattaga
     8761 attatactct attctatttc ttgacttagc atatctaggg tcaggaacag gaattgcaga
     8821 aagtagtgat tttgtatatg gatgaatagg attattatac aattcatttg aatctgccaa
     8881 ttctactaat tttccattat acataactcc tatacggtca ctaatatgtc tcaccataga
     8941 taaatcatga gctataaata aacaagttaa attcttttct tgttgcaatt ttttaaataa
     9001 atttactatt tgtgcttgta tagacacatc caaagaagct ataggctcat cagctattat
     9061 aaattctggg tctactgaaa gagctcgtgc tatgccaata cgctgtcttt gtccacctga
     9121 aaaatcagat ggatacttat ttgcataact tctatcaagt cctactagct ttagcatctc
     9181 atatacttta ttcattctat ctgttttatt tttacagata ccttgaattt ttagtggctc
     9241 tgaaattata tctgcaattg tcatacgtgg atttaatgat gaagtagaat cttgaaaaat
     9301 aatctgcata cttttgttta catctttttt tataaatttg tacgtttttt tatctgatat
     9361 gcaatttcct ttgtaaatta cttctccacc agtagattca tgtagtctta ttatagtttt
     9421 accagttgta gattttcctg aacccgactc tcctaccaat ccaaatatct cacccttata
     9481 tatatcaaaa cttatatcat caactgcttt tacagtagta cttttgtcca aatgaaaata
     9541 ttgctttaag ttttttattt ccagtatttt ttctctattc attagaaatc acccttccac
     9601 aatttactct aactggtaca tctacttcag gtgcatctgg gtgcaataac caagttgctg
     9661 cactatgtgt atcattaatt ttaaacattg gtggctcttt ttcataatct atcttaagtg
     9721 catttttatt ccttattgca aaagcatctc cttttggtgg gttcaataaa tttggaggca
     9781 ttccaggtat attatataaa tagtcatcct gcgaatctaa agtaggtaat gaacccaaca
     9841 acccccaagt atatggatgt ctagggtcat aaaatatatc ctctacagtt cctatttcta
     9901 caatctttcc tgcatacata actgctattc tatcagctat agttgcaact actcctaaat
     9961 catgagttat aaatattatt gacaaaccta ttttatgttg taaatccttt attaaatcaa
    10021 ttatttgagc ttgtatagtt acatctaaag cagttgtagg ttcatcagca atcaatacat
    10081 ctggattaca ggcaagtgct atagctatta ctatacgttg tctcattcca cctgaaaaat
    10141 gatgtggaaa ttgtttaaat cttttctcag gattatcaat tccaaccaat tcaataagct
    10201 ctatggctct cttttttgct tcacttttac ttataccttg atgaatgatt acagcttctg
    10261 caatttgctt tcctattgat atagttggat tgagagatgt catagggtct tgaaatatca
    10321 ttgatatatt ctttcctctt atatcttcca tctctttttc agacttctta accaaatcac
    10381 ttgatttaag taaaacctct ccatttttaa tatatccatt gtatgggagt atcctcatca
    10441 aactcttaca cagtacagat ttcccacaac cagattctcc aactatagcc aatgtctcac
    10501 ctttttttag attaaaagat acatttctta cagcctgaac ttctccttct tctactttaa
    10561 aacttacaga tagattatta acctccaata aatgttccaa aaaccatcaa cccctatgct
    10621 attcaaacct tagggatata tttttatccc taaggtttct aattaatttc agtattattc
    10681 aattgtccag tcagctatgt tccagaatat acctacacca tgatgaccta aaactgtatc
    10741 aggtgttaat ccttttatgt ttggttttcc aacatatatt gcatctatat aagcaataaa
    10801 tgtataaggc atatccttag tcatttctac ttggaattgt ttatataact ttaatttttc
    10861 atctttatct tctgtttctc ttgctttttg aagtatttta tctatagtag gattagagta
    10921 agcactgtaa tttgcacctt tatctgttcc aaatacttta tatgtatggt catctgggtc
    10981 aaatggactt ccccaaccta ttaaatgagc atcttgatta gcccaatctg tttcagttac
    11041 aacaacagct ttagcatcaa caccaatttc ttttaactgt tgtgcacata ttttagccat
    11101 atcaactcta acttggtcac tctcacctgc tgttatctcg aaagctaact tagtaccttc
    11161 tttttcatag attccatcag aacctaattt ccaacctagc ttttcaattt cttgtttagc
    11221 tttttctgga ttatattcaa acttttctat atcaggatta ttatatggtc ccatttgtaa
    11281 aggtgaatat gctggtacac catgtcctaa taatacactg tcaactattg cttttctatc
    11341 tattgcatag cttaaagcat ttggtaatcc tttagctttt ttatctttaa agaatttaga
    11401 attaaaattg tataatatac ctctatagtc ggctgttttc attatattta ctttaaaatt
    11461 cttttcgtct ttttcaaagt tagacatatc tttaggagtt atttgagcta ggtctaattc
    11521 tcctgatttt aattgcattg ccttagcctt atcatctgga acaatcttaa agactacttt
    11581 atctaatcct ggttctttta caaaataatc actattctta acaagtgtta tactttgacc
    11641 tttgtcccat ttttcaagtt taaatggtcc agtacctatt ggtttttgat taaattcatc
    11701 agtagctata tccttacctt ccaaagcatg ttttggtaat actcctactg ttaaataatc
    11761 aagcattgct gtatttggag cttttaaagt aatttttata gtattatcat taaccacatc
    11821 aatttttgtt atatcttcat aatttgatgc tatctcagaa gcattatctg gattcattat
    11881 agtttctatt gtaaacttaa catcatttgc tgtaaatttt tctccatcat gccacttaac
    11941 atcatctctt aaattaaaag tatatgtatt tgttgcctca tcaaatttcc agtcctttgc
    12001 tagacaagga accaccttat tattttcatc atgagctgtt aatccattaa atattaacga
    12061 atttatttct ccatgttcgt atagtgcagg atttatactt gtatagtcat tacttccata
    12121 aacaagtacc tttccatctt taggcgtatc tgccttctta tctttgtctc ctccactaga
    12181 acaacctgcc attagactaa gtatcattac taaacttaaa acttttaact tctttaattt
    12241 catgatgctc ccccttttgc tttttataaa atatttttca aagctttgac cccatttatt
    12301 taaatagata tttcaaatac ctcagtattt gcttatttaa tactcaaatg ctgatttata
    12361 aattactaga ttttttatta ttattctttc ttatataatt acctatattt gttatacaca
    12421 ccaatgtggt aaccaaaaat atacccggaa tcagtataat ccaccatcta tttgaaagta
    12481 atgcttcctc tgacaaagat aacatacttc cccaagaaac aatttctatt ggtaaaccaa
    12541 ttcctaaaaa gctaagtgtc gattcagtac caatagctgc gcctatgttt gttacaacca
    12601 tgaacattat tgatgccaca aaatttggaa gtaaatgttg ctttaataca tagaagaatc
    12661 ctcctcccat gctcttagct gctagtatgt attcgctatt ttttatttgt ctaacttctg
    12721 ttctcactat cttagatata ttcatccaac ttgttatccc tattactatg gacatagata
    12781 ctggatttga atttccaagt atagcttgta caaatattat aattaatatt gaaggtatac
    12841 taagtatgat ttctgtaaac ctcatcattg catcatctac aagttcagat gctgaaccac
    12901 ttatactgcc ataaacaatt cctataacag ttgatattac agtggaaaat agtcctataa
    12961 ataaagatat ttttcctcca taccatatca tagaatatat atctctaccc attgagtctg
    13021 ttccaaataa aaaagtttta tttggagcta aattagaact tgctaaatcc atgtatgttg
    13081 gttcatgtgt cattataaaa gatgagaata cacttccaat gactatgatt gataaaataa
    13141 taattgatat atatggtaat tctttaattt ttttagataa ggttttcttt gtttctatat
    13201 gttcatcttc tgctactaaa atataatttt ctcctacaat ttcaaaatca ctatattgtg
    13261 gcttcattag tatcacctct atcatatttc attctagggt ctattttatt gtttataatt
    13321 tgagctaaca tattcccaaa tactactaaa gcccctgtta ttaagcataa aactaataac
    13381 atattataat catgatactt tgcactttca aaacaaagtg ttcctaaccc aggataagaa
    13441 aaaacttttt caactacata agttcctcct agtatatgag gtatcgaaat ggccattata
    13501 ctaatatatg atggcattat atttctcagg caatgcttaa aaacaatagt tctattattt
    13561 aaaccttttg ctttacataa aagcacataa tcttctctta tttcctctag taatttattt
    13621 cttatcatat atgtgtaata ccatagatgg cttaatataa gtactgtaag aggtaagatt
    13681 agatgtgata tcctacttaa tatactggac tcttctccca ttgaataagc accactactt
    13741 ggaagtatac ttaagtttat actaaatata agtatgagta ctaaggcaac ccaaaatgat
    13801 ggaatacagt tggttattgt tcctaatttg catatgattc tatcaatgag cttatcttca
    13861 tgtaatgtac aaaatattcc taacaataag gctagcacaa aggtaagtat atatcctgaa
    13921 ccacctaata ttatagtgtt tatatagaca tcatttataa cagaagtaac attttgttta
    13981 tatttaaatg aaattccaaa ttcccctttt gatgcatttg aaatccattt tatatattga
    14041 ctgtatattg gttcatccaa accaagtttt ttcatagcat tttctttttc ttgtgtactc
    14101 attctttcta ctccatcccc atagtaagag attaatgggt ctcctggagc aagtcttgcc
    14161 atgtaaaaaa caataaaaga taataaaaac attactaaaa taaactgcaa caactttttc
    14221 aatatatcct ttaacaaacc ccaacccccc tttgttgcaa taaaaaaaac cacataaaag
    14281 caaacgtaaa gtaacgttaa aaccttcgtg gttccattat cattaaacaa aattaaattt
    14341 tctatattta tctatgagtt aattttagca tcatttatta actgtgtaaa caaacttttt
    14401 aaaaattgtt aaaatttcat caaattattt tatttatacc taaaataaaa tttttatatt
    14461 ctattcttca ttattttcta taaaattata aaattttagg ttgactatat aagaaaagta
    14521 tattatatta gtttgtatgc aaaccattta agaggtgatt ttattgaaaa gtaattatga
    14581 ctgttttaga atagccatgc tcttaaaaga actttattct aaaactatgt acacagtaga
    14641 agaaaatttt aaagaaaatg gattaacaca ccaacagatt atagttataa aactgatagc
    14701 tcataatcaa gaacttacaa tatcacaact atgtgatgaa atgtctttag ctaaaggtac
    14761 ggtttcaggc ataatcagca gattagaaca gattggatat atagaaaaat tcaagaaatc
    14821 taatgataaa agaaatacat acgttaagtt tacaacaaca ggatttgaat ttgccacaaa
    14881 ttttaaaatt aaaatgcaag aaagttttga tgatattttt aaaaattgtg atgaaaacga
    14941 attgagtgac ttagtaaaaa atcttagaaa tatattggca aaagtaaagg agagataatt
    15001 atgaattata aattaatact tgcaatagta tttataacat cagctttaat tttctacact
    15061 ataggtgtat ttggagaaag aaaggcaaaa attttaaaaa agaaacatgt aattatattt
    15121 tggcttggtt ttatatttga tactttagga acatttacaa tgagtaacat agccaacagc
    15181 catacttttg aagtaaaatc tgctttatca caaaaccttc atagtattac aggtcttctt
    15241 gcaattgtac ttatgctttt tcatgcaagt tgggctactt ttgtcctata caaagatgat
    15301 gaggaaaaga aaaaattctt tcataagttt agtatagttg tatggactat atggttagtt
    15361 ccatatttta taggtatgtt tattggtatg gctggatagt aaaataaaaa gtgtgaaaga
    15421 aggatgtagg taaatttgta taagacaaat ttacctacat ccttctttca cacttttaca
    15481 tatttattta taatactcca ttatttcttt catgtttctt aatacctata tagaatattt
    15541 aattgtttat atagtatttt attattttct cacacttttt catatatatt gttttactat
    15601 ttcttcatat gtatatatga aatattccat tatttctata
//
LOCUS       DAEORZ010000097        15506 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEORZ010000097 DAEORZ010000000
VERSION     DAEORZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15506
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.097"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            70..921
                     /locus_tag="KRH39_002638"
     CDS             70..921
                     /locus_tag="KRH39_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBG8512530.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1054..2667)
                     /gene="ggt"
                     /locus_tag="KRH39_002639"
     CDS             complement(1054..2667)
                     /gene="ggt"
                     /locus_tag="KRH39_002639"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBG8512531.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKYGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2892..4829)
                     /locus_tag="KRH39_002640"
     CDS             complement(2892..4829)
                     /locus_tag="KRH39_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512532.1"
                     /translation="MTLFRIAMQNVKNSFFNYLIYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMILVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLITISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEIEKINLHIKDANEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4816..5583)
                     /locus_tag="KRH39_002641"
     CDS             complement(4816..5583)
                     /locus_tag="KRH39_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512533.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5871..6545
                     /locus_tag="KRH39_002642"
     CDS             5871..6545
                     /locus_tag="KRH39_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8512534.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHKGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            6569..7573
                     /locus_tag="KRH39_002643"
     CDS             6569..7573
                     /locus_tag="KRH39_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8512535.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            7685..8914
                     /locus_tag="KRH39_002644"
     CDS             7685..8914
                     /locus_tag="KRH39_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG8512536.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSNFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            8922..9596
                     /locus_tag="KRH39_002645"
     CDS             8922..9596
                     /locus_tag="KRH39_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512537.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            9599..10831
                     /locus_tag="KRH39_002646"
     CDS             9599..10831
                     /locus_tag="KRH39_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512538.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(11117..12382)
                     /locus_tag="KRH39_002647"
     CDS             complement(11117..12382)
                     /locus_tag="KRH39_002647"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBG8512539.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNIVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(12435..13583)
                     /locus_tag="KRH39_002648"
     CDS             complement(12435..13583)
                     /locus_tag="KRH39_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG8512540.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(13683..14228)
                     /locus_tag="KRH39_002649"
     CDS             complement(13683..14228)
                     /locus_tag="KRH39_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG8512541.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(14451..15395)
                     /locus_tag="KRH39_002650"
     CDS             complement(14451..15395)
                     /locus_tag="KRH39_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG8512542.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMCKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         5433 a         2406 c         1947 g         5720 t
ORIGIN      
        1 attagtttat aaagttaata attaatataa attaaaataa agataatata aacacaggag
       61 gtacattctt tgaaaaacat tatttcagca aaagaactta tatcaaaact agaaaaaaat
      121 gataatctag ttgtaataga ttgtagattt gatcttatca atagaactta tggtattgat
      181 tcttataaaa aaggacacat caaaggcagt tttatcttag atatagacaa ggatttatca
      241 tctcctacaa aagaacatgg tggtaaaaat cctttacaag accctttaat actaaaagaa
      301 aaattagaaa aaatgggtgt tgataatgac actacgattg taacttatga tgatggagat
      361 ttaaatggag cttgcagatt atttttccaa ttaaaacatt tggggcttaa aaatgtctat
      421 gtactggatg ggggaataac atctttcgta aaggaaggtg gagaattgga agagaaagtg
      481 aatataccaa gttgtacagg taaggaaatt agaccaaata taaacaatga tttagttgtt
      541 ccaatggaat atgttaagtc taaattatac aaaaaagata ctgtaataat agattgccgt
      601 gctaatgaga gataccaagg tttagttgaa cctgcttact caaaagctgg tcatatacca
      661 agtgcaaaaa attatttttg taaggattta ataaaaagtg actttgaaaa tggctcactt
      721 aaagatatag aatttttaaa aaaattcttt aaagatttaa ataattatga tgaagtaatt
      781 ttatcttgtg gttcagggat atctgcttgt gttaacagtc ttgctcttag agaactggat
      841 atacctcaca agatttatat tggtagcttt agtgactgga ttagttatga tgacaatgaa
      901 atcaaaactg gtcaagaata aggctcataa tctatataaa atcagcatat gataacttaa
      961 attttgtaaa ggcgtcagtc attgtagact ttcgccttta cttattattt catatactct
     1021 ttaaatttat tatatatatt ttagttgata atattaataa actgcaatat gaccatcaca
     1081 tcttgattcc gtcccacaaa tataactttc ctgttctgtt ttaaatatta tttgacctcg
     1141 tcccatgtca acagtatcat gaattacttt tatttcatga cccttttcaa ttaatgaatc
     1201 tattatatgc tttggcatct ctggttcaac ttctatttcc tttcctttta tccactgcca
     1261 tcttggagca tctagcgcct cttgtggatt taatccaaaa tcaatcatat ttgttaacac
     1321 ttgaatatgt ccttgtggtt gcataaaagc tcccattaca ccaaatggac ctattgcttt
     1381 atcttcttta cctaaaaatc caggtattat agtatgataa ggttttttaa atggttttac
     1441 tacattgtgg tgtttagggt ctaaattaaa attatttcct ctactatgta aagcaatccc
     1501 agttcttggt atcacaattc ctgaaccaaa atttatataa ttgctctgta tatatgaaac
     1561 catatttcca tacttatctg cagtacaaag gtatactgta ccaccacaat atggctctcc
     1621 tgcagttgga tataaagcct ctttattgtc aatcaataaa cttctttttt tggcatactc
     1681 ttggcttagt aattcctgaa tctttacctt catatgctct atatcagtta catatgtttt
     1741 agaatctgca aaagctaatt ttaaactctc tataatttta tgtatgtctt ctacattttc
     1801 tatatttcct tctaattcca attcttttaa tatgttaagt gccattaaaa ctgttattcc
     1861 atgaccattt ggtgggattt caaatatttt atatccctta tattctgtac ttataggttc
     1921 tacccatgtt ggataaaaac actctaagtc actctttctt atagccccat taaatttttt
     1981 agaatattca tctattttat cagcaagtct tcctctataa aagctttcag cctgagtatt
     2041 tgctatctct tctaatgtac ttgcctgttc ttcacatata aatatatctc cagcctctgg
     2101 agctttacca tctttagaaa atgtatcaaa ccatggtttg aactcttctc caataagttc
     2161 tttttcatag agttcatatg attttttcca taccttagct acattaggag atactacgta
     2221 tccctctctt gcatagttta tcgctggaga taagcattcc attaagctaa gtttaccata
     2281 ttttttattt aattctgacc aagctgctgg tattccaggt acagttactg ctccaaaccc
     2341 atattttggc atttctttca tattattata attttttaat tccatatttt ctggagcaaa
     2401 gccactagaa tttaaaccat acaatcgttt ttcttcctct atccaaacaa gggcatatgc
     2461 atctcctcca atcccattgc ttgtaggttc tactactgct aatgtggcgg ctgttgcaac
     2521 tgctgcatca acagcatttc cacccttttt tagtatttca agtccagctt gagatgctaa
     2581 tggtgttgat gttgcaactg ctccattttt agcatacacg acatttctta ttgatgagta
     2641 tttatgttgg taggcattaa acttcataaa tttcagctcc tcgtcatttt ttatttgata
     2701 ttctgcttag atttattata taacatcaag taatgtaact gatacaaaat gatataaaat
     2761 atagactttt tcctaacttt aattttatct ttaaataaat atcttaaact caattctagt
     2821 attttctatc tgcacttaaa taagttattt tattcaaaac atatatctaa ttcttttaac
     2881 ttaaaaataa tttattcttt tatcatgttt agagcatatt ttaaagttaa aaaataatat
     2941 ataaaatatc ctataaaata aatgactaga gttatgataa ttggtacaag taatgacctt
     3001 ggtataaact tttgtgctat agacatagca aataaattat gaactgtacc tacaactaat
     3061 ggcattaaaa atataacttt taactgtttt gatattattt tctcaatatc tttattattt
     3121 gctccaagtt ttattaacat aatatatctt tccttatcat catatatatt tgataattgt
     3181 ttaaataaga ttatactacc tgtacataaa agaaatacta cagacaaaaa tatgcctata
     3241 aacagcatta gaccacttaa ttttatcaag ttattataac tttcaatact tgatgtaaaa
     3301 ttaaaaggat aagtaaattt catattttca cttactatat tttttaattt taaagtcaaa
     3361 tccagttctt ttttttcatt ttttatatca attaatctta ttaatctaga atttccatgt
     3421 aattttaact gattaaatac gtcatctttt actacaatta aatcaaacgt agcttgtgaa
     3481 tttatttctg gctccaagat gcttttttgt actttaaaca gatttatatc atactcattt
     3541 gcatctttaa tatgcaagtt tattttttca atctcaaagt ctttaaaaaa tatttttttg
     3601 tatgaatcag aaacatagta cacagattcc tttgaagtta attcttcata attgttattc
     3661 tgatattgtc ttaatttttt aaaatcactt tctctaataa tatctacaga cgttctatct
     3721 tctatataac tatttccatt tttataatac atttcaatat ttatatcttt gtctataagt
     3781 tctattgttt tatcaaagat tatcttatta tcttttttat atttatataa taatttgtca
     3841 atttcgttat ttacataaga atttcctaaa tttatggtat aactgtattt ataattttca
     3901 cttatgttcc tatctatatc gtaataaagt gatatggtaa accctagcat agttacactt
     3961 gtagctatta atattgctat tacagccaat acactactat tgctctttaa tttaaagcca
     4021 agttcagaaa atgcaattaa attctgtccc ttataataaa aactttttct cttcttaaca
     4081 gcatcaataa tcatatacat agttgatgaa aaaaataaaa aagttccagg tattattgta
     4141 actaatataa aaactgaaag cataatattt tcagcaataa aataactaat tgacattaaa
     4201 taagaaatag ttattaataa aacaccaagt acacatttta actttataaa ttttttaaac
     4261 ttatactttt ctggatattt attaaataac tctataattc tcttatttct gataactata
     4321 gtatttctta ttccaataac tataaatatt attaaaaata ctgctagtgt ctgaactaag
     4381 gcctttatac ttaagttcat atttactaat atcatttcat tcattaattt tacaagcatc
     4441 atagtcataa gctttgaaaa taacaatcct aaaaatatac ctattagtat tgcaactata
     4501 cccatcaaaa aagtttcata taaaaatatt tttcctatct gatttttcct cattccaagt
     4561 aaactataag ttcctacttc tcgcctacgt ctattgaaaa aaaatgagtt tgaataatat
     4621 ataaataaaa atacaaatac cactattaca attgaacttg tattaatact catcctggtt
     4681 ttctctccaa gtgaactcaa cgcttcatta tactctatag atttaaaact aaaaaataca
     4741 aatacactaa atacaattga aataaagtat attaaataat taaaaaaact attctttaca
     4801 ttttgcattg caattctaaa taaagtcatt tttattaact cctccaatta aggaaactgt
     4861 atttactatt ttattataga actctctgtt actttctccc tttacaattt ctgtgaatat
     4921 tattccatcc tttataaaga gtattctatt gcaaaaactc gcacttatag catcatgagt
     4981 taccatcaat attgttttat tgctgtcttt gtttaatttt aatagacaat tcaacaaatc
     5041 cctagcagat ttagaatcta atgctcctgt gggctcatct gcaagtacta atgatggttt
     5101 tgttgctata gctctacata cagctcctct ttgcttttgt ccaccagaaa cttcgccagg
     5161 gtatttatct aatatagatt ctatatttaa ttcttttgaa atctctttta ttctattttc
     5221 tattactgat actctttcat tttttaaaga aagaggcaac attatatttt cttttattgt
     5281 aagtgtatcc aaaagattag aatcttgaaa tacaaagcct agattatcac gtctaaaatc
     5341 tgccaattca ttttgcttca ttttaatagt attctttcca tttatataaa tatctcctga
     5401 tgaaggtaaa tctattgtag aaataatatt taaaagtgtt gatttaccag caccggaaga
     5461 ccccataata ccaacaaatt ctcctttata aaccttgaaa gttattcctc ttagagcttc
     5521 atgttttgaa ccttttcttc catactcctt ttttatattt tcaacagata atatttcttc
     5581 catagttaat aatcaccttt catcacaatt agttatttat caattctcac aaatatatta
     5641 tatatattaa aatggatttt aaacatttaa ttatattaat ttaaggttac attttcgtaa
     5701 gtattaatta tatttcttca aatttattgc aattatgttt aaaatataat ttatcaagta
     5761 aaatatagtt atattaatat aattatttta ggtaataagg tagaaatatt ttaatttatc
     5821 aagtagaata tagttatgtt aatataacta ttttgaatta ggaggtaaga atgtttaaaa
     5881 taatggtagt tgaagatgac gtctcattga aaaatatcat agcaaagtgt cttactaaat
     5941 ggggacatga tgttcatcaa atagaaaatc ttgaaaatat aatagaagag ttcaaaaact
     6001 ataacccaga attagtttta ttggacataa accttccgtt ttatgatggt tttcattggt
     6061 gtaatgaaat aagaaaaatt tcaaaagttc ctataatttt tatttcttca agaaattcta
     6121 atatggatgt aataatgggt gtaaatttag gtgcagatga ttatatacaa aaaccttttt
     6181 ctgtagatgt cttagtagct aaagttaatg cccttttaag aagaacttat aattttgttg
     6241 ataataactc aaatcagatt attcacaaag gtgttacttt agatttatca actgcaacaa
     6301 taaactatga agataatact attgagctaa caaaaaatga aatcaaaatt ctccatgagc
     6361 ttatgaaata taaaggtcag atagtaagta ggaataaact catgaaaaaa ttatgggata
     6421 atgattggtt tgtagatgat aatacattaa ctgtcaatgt aaatagaata cgctcaaaat
     6481 taaatgaaat tggtttagaa gattttatag aaacaaaaag aggattgggt tatataatca
     6541 gttaaaacaa ttctaagtag gtgaatttat gagctttaaa gattttctca aggataaact
     6601 aggtttttta gtatacaatt ttactttctt aatttttaca gtatcagtta ttatattttc
     6661 tcctgtagat gtttttctga ctgatactat tttatatata ttagttgtaa atgttgtgtt
     6721 tttattttta tatctattta ttagctacat caagaagaac aaatttttag ataagataaa
     6781 aaatgataca ttcaaaatta atatgaatga tatagaatta gctagatgta aaaatgaaga
     6841 aaaaatatac ttgaacatta ttaaaaatca tcaatatcaa tgtaataaca taataaataa
     6901 taatatcgag aaatttgaag aaaacaaaga gattttaaat atgtgggtac atgatgtaaa
     6961 gatacctata gctataataa aactcattct tgaacaaaat gaaaatttaa ttgatgaaaa
     7021 aatttcagat gacatagata gtgaaatatt tagaattgaa aactccgttg aaaaaatttt
     7081 atatttatca agactcgaag attttcataa ggatttttta attcaagagg taaatattga
     7141 aaaaatcatt agaaatatta taaaaaaata ctcaaaatat tttatttcaa aaaaaattat
     7201 tttagactta aataatttga attttaaggt tttatctgat gaaaaatggc tatacttcat
     7261 attcgagcaa ttattgagta attccctaaa atactcctca ttaggagata atatatctat
     7321 ctattgcaaa agaaccaaaa attatttaca attaagagtg gaagatactg gttgtggtat
     7381 aaaaaaggaa gatttaaatc gaatattcaa taagggattt acaggtaata atggaagaaa
     7441 caattctaaa tctacaggtc ttgggcttta tttagtaaaa gaactttgtg ataagcttgg
     7501 tcataaaatt gatgtagatt cagagtacaa ccaatataca aaatttacta ttacattcaa
     7561 atgtgattta tagtatctta ttattgatag tttattttca ttaacttata ttcaacttct
     7621 tatagtaatt ctatatgtac atcccagtat ataattaagt agtttttata aaaaggagga
     7681 agacatgtct tttctaaaaa aatttaaaaa taaaagattt agaaataaca gttttaaaag
     7741 taacaacttt agaaatagca gttttggaag tagtggcttc aagtctaaaa ataaaggttt
     7801 taaaaacaat ccctttaaaa ataagagatt caaaatatat gcaggaatag cagtaatcat
     7861 acttttaatt ctaggagttc ttgcttatgt tgattcgaaa aacactgaat tacaagctaa
     7921 tgaaaatttt atcgaaacat atactattcc tgaaaatgaa aaaatattta taaatgggat
     7981 ggttgttcct aagcaaacta aggacttcaa tatatctgga gattatgagt taagtgatgt
     8041 taatgttaca aatggtcaaa aagtaaatca aggagacctt ctttttactg ctaaaaatcc
     8101 aactataata gcagaaatag atagtttaaa atcacaactt agtcaataca aaaaacaaaa
     8161 aatatcactt tctgatattg ctgaaaataa agatgctatt gcttctataa atgcacagat
     8221 aactgcttta aactctcaga tagcttctct ggataaaaaa gcttatgaca gagttactgc
     8281 tccttttgat ggcacagtat acttgaatga ccaaactgga aatccagacc aaccagtttc
     8341 atttatgact attcaaggtc tagagtttta tatgaaagga caagcaagtg aacaagattt
     8401 acctaaaatg aagattgacc aaatggtcaa tatactagtt ttttcaactg accaaaagct
     8461 tactggtaga ataagtttta tatctgataa accatctact ccaaatactg aaatgggagc
     8521 tcagcagaat acactatctt attatgatat aaacatagca tttgataatc aagaaggatt
     8581 agtaaatgga ttccatcttc aagcatcact tgaagtagct aattcaaact ttaaaatacc
     8641 agcatcttgt gtacttaaag acaacaaaca ctcatatgta tttaaggatt tagatggtat
     8701 attgaaaaag caaattgtag atgtagcaag tcaaaatgat gattttgctg tagttagagg
     8761 cggtcttgaa caaggcgata taataataaa acaccctact aaagaaatga aagaaggaga
     8821 cccagttcaa ggcgatggcg taactgctgg ttctaataat ggagatacta ctccaaacaa
     8881 aaaagtagaa gaagttacta tggatgtgaa ttaatttaca tatgttaatt aaattagaaa
     8941 atattcaaaa atattacaaa gtagggaaag atgaattaca tgttttaaaa tccttaaatt
     9001 tagaaattga atctggagaa tttgtcatga taatgggtaa gtctggtagt ggtaaaacta
     9061 ctttacttaa tatcttaggt tttctagatg tatttgatga aggaagatat atatttgatg
     9121 gaacagatgt aactaatttg agtgaaaatg aacgttctgt ttttagaaat attaatattg
     9181 gatttgtttt ccaacaattt aatttaatag aaactctaaa tgtatatcaa aatgtagagt
     9241 tgcctctaat ttacaataaa gcactaaaaa aatctaatag agaagaaatt gttaaagata
     9301 aattaagttc tgttgggctt ttggacaagc ttaagcagaa accacttcaa ttatctggtg
     9361 gtcaacaaca gcgtgtagcc attgcacgtt gcctagcaaa tgaccctcag ataatctttg
     9421 ctgacgaacc gactggggca ttagatagtg aaactagcag agaaattatg gagcttttaa
     9481 caaggctcaa caaacaaggt aagactataa taatggttac acatgatcaa gatttaacta
     9541 agtatgcaac taaggttata cgacttaaag atggtgtgtt tacttcggag gtgtaatcat
     9601 gagtttatta aaaaattctt tggccaattt aaaaggtcac aagcttcgtg tatttgtagc
     9661 tttactctgg attataatag gaataacttc agtaatactt gttagttcta taggtaatgg
     9721 atttcaaaaa gaaattaaga aatctgtaaa caatgtaaac cctaataaaa ctactatttc
     9781 gtttgaatca gccgataaca caggtcttac tgatgatatg agtatttttt taaaaccctt
     9841 taatgcaaaa gatttagaag aattatcatt tgttgaagga gtagaaagaa tagctccttc
     9901 aagagatggg tttaaccttg attcagttta ctcttcacaa gcatcttttg ataaaaaaac
     9961 tacgtatgtt gatgttggac cagtaaaaaa agattctaaa ataaatttaa tttgtggtag
    10021 agatttttct ttagatgatg aaaaaagaaa agtaatttta cttacattac aaagtacaag
    10081 tgaaattttt gaaaatccag aagatgcctt aggtcatggt atcaacataa atggaactat
    10141 atttgaaata ataggggtat tagatgattc tcagcaaaac caagctggag gattctttgg
    10201 aggttaccaa gatatgcaat ttactacttc cctagtacct aaaaaggctt ttgacacttt
    10261 aatgagtcaa aattcttatt caaatgaaat ttaccagtta gacttagttt cttctaaagg
    10321 ctataatgta aatgaagttg caaacaatgt tatagctaaa ttatatgaga tgcaccctgg
    10381 tatcaatggt tcttatacta caccagaccc aactgaacag acagcttatc ttgaaagcat
    10441 taattccaat gtaaacaaat atgtatctat aataactgta gttgcaatgt ttgttggtgg
    10501 tattggtgta atgaatataa tgtatgtatc tgttatggaa agacaaagag aaattggtat
    10561 aagacgtgct attggagcaa aacctaggtc tatattattt caatttttag ttgaagctgt
    10621 ttttataaca gtatgtggtg gaattttagg aactatagtt ggatttgctg ctactaacta
    10681 tgtttctaaa tatataggtt ttgaagctat acctagctta aatagcttat tttatgcaat
    10741 tgtagctact atattgactg gagttgtatt tggtctgatt ccagccttta aagcttctaa
    10801 actagaccct ataaaggcaa tttataagta gaaattgtat aaagattttc gatttttaga
    10861 ttagtttata taaaacatac ttaaatatat aacgcactat tatatatgat agattaggaa
    10921 cttaatcttt ccaaatataa tagtgcgttc tttcataaaa aggattctct tccttactat
    10981 atctaaatta ccaagatgtt atgttctaat tgttcaaact aatacttcca attttgatta
    11041 atcaatctca ttctgctaat atacaatctt ttactttcaa tcattgtaca ctccgcctta
    11101 aaaaaataat cttaaattag gcgttatata caatctcccc attacatatt gtataattaa
    11161 cctttccata taaagattta cctttaaatg gtgaattatc agctttagaa acaaagcttt
    11221 caactgtcca ttcttcttct ggattgaata taacaatgtc tgctacagcc ccttcttcta
    11281 taaatccaat atttaaattg tacaactttg caggatttat agacattttt tctattaact
    11341 gcatcatact aagatgattt ttatgcacca aatttgttat tccaagagct aaagaagttt
    11401 caagcccaat aattccactt ggagctttta taaactctct atctttttct tctttactat
    11461 gaggtgcatg gtccgttgct attatttcaa tagtaccatc atttaatccc tctataatct
    11521 tatctctgtc ccatttactt cttagaggtg gattcatttt tgcatttgtt ccaaactcca
    11581 atacatcttc ctcagtcaat gtaaagtgat gtggtgaagc ttctgcaact acatttgcac
    11641 ctaagtattt tgcaaatcta accatatcaa cagatacacc tgaacttata tgttgtatat
    11701 caactttagc accagtcttt agtgccaaca tacaatctct ggctaccata acatcttctg
    11761 ctacacttga tgcacctttt aatccaagtt ctgaagctac ctttcctgca tttacacctg
    11821 gatttccaac taaagaaggg tcttcttcat gaaaactaag tggtacatcc aactcttttg
    11881 ccttaatcat tgcctccata attagattgc tatccattaa aggtaatcca tcatcagtaa
    11941 accctggtac accagctttt ttaagagctt ccatatcaac taactcttta ccttcaaaac
    12001 ctttagttat agctgctgct tgtaaaacat ttatacatgc attttttgac ttttctttaa
    12061 cataattaaa agtatcttca ttatctacaa taggattagt atttgccata catataacag
    12121 ttgtaaatcc acctctagca gcagatttag ctccactttc tatatcttct ttatatgtaa
    12181 acccagggtc tctaaagtgt acatgtacat ccactaaacc tggagcaact atattaccac
    12241 tagcatctat tattttttca tattcatcac tttcatcaaa cttgcctatt ttataaattt
    12301 tattttcttt tataattaag tcaatattct catctctttg acttttaggg tctattactc
    12361 taccattttt aattaaaatc atattatttc tcctttatat ttttaattta tatagactca
    12421 tataatatta tacattattt tacatactta aatacaaaat tacgtttgac atcaatagcc
    12481 tttttaggac aaagttcatg gcaacagtaa catcttatac atttacttaa gtctataata
    12541 gcacccttag tccctatctc tatagtggat gcaggacaag cttccttgca ttttccacat
    12601 tttatgcaat cttgaaatct taccacaggc tttggtgtta taagctttga aataggttca
    12661 tttaaaaatt ttggtagctt tacagaaaac aatcttaaat ccttacttac atttggaatc
    12721 ttgtaatttt ttataattaa atcttttata tcttcaccta aaatttcaat atctgaaaaa
    12781 tcttcttgta tatatcctct cttaatagca cctctaattg tagcaactct atttggaagt
    12841 aaacttataa tcttacatgc aacaacatct atagcatatg gattttgtga tgctaataaa
    12901 actcctattt ctcgagtagt accagctgaa ggtccatcac cttccatacc tactatacca
    12961 tccataattg tcaatgttgg ttttatataa tcacaaatat ctaaaagtac attttcgcaa
    13021 aacaactctt ctgtaggaaa tctataatgt atctctgctt ttttcaatcc agctatgcat
    13081 ccatataagt tttttgtacc accagtaaaa gttgccatta tatgagtctt taatttacaa
    13141 agatttatta tataatctga atctactatt ggtttaataa tatccatata ttttagaaca
    13201 tgtgcttttt cactatgtat cttaacatct gaaacatcat aatttaactt aatgcccagt
    13261 tcatttgcta cttcttcaat tccagatgcc ttataaatgc tcttaagaga actttgatta
    13321 tatggaccac cagggctatc tgctattgta atatcacaat ttaactctaa aagtttttca
    13381 catacaactt ttacaaccat aggatgtgta gttgtagctt cttcaggctt ttttttcatt
    13441 aataaatttg gttttataag tactttgctt ccttttttaa caaacttatc tataccacca
    13501 atatcctcta aatttttttc aatagataac tttacttctt cataattgta agttttacaa
    13561 ctcctaacag ataccgactt catattatct ccatccttct atcacaacag tttatttgta
    13621 taatttaaaa aaggagaaac caaagtttct ccttttaatc tatgttttaa ttgttttaat
    13681 tattattttc caaaatgtct gtttaataat cctaagaatg ctttaccatg tctagcttcg
    13741 tctttacaca tttcatgtac tgtatcatgt atagcatcta atcctaattc tttagctctc
    13801 ttagctaatt ttaattttcc atcagttgca ccatactcag catcaactct aactcttaag
    13861 ttttcttttg tatctgcaac tacaacttct ccaagaagtt ctgcaaattt agcagcatgt
    13921 tcagcctctt caaaagctat tctcttatac gcttcagcta cttctggata accttctcta
    13981 tcagcttgtc tactcattgc taaatacatt cctacttctg tacactcacc agtaaagttg
    14041 gctcttaatc cttcgattat ttctgcatct acaccttgag ctactcctat tctatgttca
    14101 tcagcccaaa ccatttcgcc tttcatttct tcaaatttat cagctccaac tttacatact
    14161 ggacattgtg ctggtgcagc atctccttca tgtatatatc cacatactgt acaaacaaat
    14221 tttttcataa ttaatttcct cccaatttta tataattaaa tttttatttt taagtttaat
    14281 aacttgctac aagaatatat ataccccaga ctttatagct taaacactaa aaataaaatt
    14341 tatttcctaa ttaattttat cactttgaaa caccagtatc aaccaagttt tgatgtcaaa
    14401 tttctacata atatataatt tatttgatta attaggaaat agttgtcata ttaaatactt
    14461 tctaatgaag actcctctga atctttaagt ttcacttttt tgctctttaa aaaagataat
    14521 ttagtttctg cagttatgat tgcaacaaat atcaagatac taccaataac caattttgct
    14581 gtaataagtt cctttagaat tattatagaa aatattgtac caaatacagc ctctgtggat
    14641 aatattatag ctgttctagt tccatcaact cttttttgac atatagtctg aaataaaaat
    14701 ccaattgtag tactaaatac agctagatac atagttccca tgtaaccaga taattcagct
    14761 tccatcttta attgacctgc aaatgtttga actacaacag acattaaaaa cgctactgta
    14821 aattgaatag ctgtcaatgc cattgggttg ttatcttttg caaactcact tgtaaagaat
    14881 atatgaaatg caaatccgaa tgaacatata agtgttagaa aatctccaaa attaatagag
    14941 aaatcagctt ctaaagaaag tatcccaatt cctataagag ctaccaaact acttattatt
    15001 cctattttat caagttttct tctataaagt ataaatccta tgaatggaac tattacaaca
    15061 ttagctgctg ttataaacgc atttttagat ggtgtagtgt acataagtcc aatagtttgt
    15121 actgtgaatg ctagaaataa aaatattcct aaaatccctc ctgcttttaa taatttttta
    15181 cacatattgg cacgaatctg tttaaaaaac agtaaattca taattatagc tgccaagaaa
    15241 aatcttaaag ttatgatttg tagaggtgta agcccaccgt ctaatgcaaa ttgggttgct
    15301 acaaagccac ttccccatat aattgctata aatattagtc ctatttctcc aaagtatttt
    15361 ttcaataaag ccccctcctt tttatttttt tgcaaaatta taaaccccct aaacagcaag
    15421 agagtttttc tataagcatt actataaatt aatatagtaa attttttcag tattttaaat
    15481 ctaaatttac atacattttg agctca
//
LOCUS       DAEORZ010000098        15387 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEORZ010000098 DAEORZ010000000
VERSION     DAEORZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.098"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            164..2365
                     /gene="slpA"
                     /locus_tag="KRH39_002651"
     CDS             164..2365
                     /gene="slpA"
                     /locus_tag="KRH39_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBG8512543.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPIFADTTVKEEGYTVVQDKYEKLL
                     KELKARINDGTITSVGVEFDGSPIGTVAPESDSTEHKKAAADKLETLTKNQLKGLGDG
                     KYVDFKINYGAKDAIPAPTLSAKDIDALKAKLDEMKTKQLVDVAKDGQPGIAKVDGSD
                     VIAGDAPLTIADAVTVTEGTDSGKKTLTIAAASTLSNFKYGALKTGGADASSVSAITL
                     STSNLVIKEGEAKKVNLEESLKFNSDTKVISGFDVTTLNETPADPGSSTIVRVIKAVE
                     KTIDVSSSSTTKAKDLAKQYVFKDGDAETPGNLSYMLKYINDEDKKVAVKNNDGDYEV
                     TIFPEGKRLNTLSASSAKTILGEADAPAKIVLKASSTKKLADYIDDLITYNNSYSNVQ
                     TVAGSDRIETAIELSRKYYNSTDKNALYGNPVNNVVLVGSQAIVDGLVASPLAAEKDA
                     PLLLSSKDKLDSSTRAEIKRVMDLNSSTGIKNNKEVFIAGGVNSISKDVENELKDMGL
                     KVTRLSGDDRYETSLAIADEIDINDKAYVVGGTGLADAMSIAPVASQIKDGEATPIVV
                     VDGKSDKLSKEAEDFLDDAQVDIIGGENSVSAKMEDYIDDATGKSPERISGADRQATN
                     AEVIKEYFDKDKVSNYFLAKDGSTKEDQLVDALAAAAVAGNYGSKHNANGNITTDASP
                     APIILATDNLSAEQHVAVSKTSNSDVAKNLVQVGQGIADSVVSKLKDLLDM"
     gene            2611..4953
                     /gene="secA"
                     /locus_tag="KRH39_002652"
     CDS             2611..4953
                     /gene="secA"
                     /locus_tag="KRH39_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG8512544.1"
                     /translation="MSVLDVILDKTDELEIKKLNNIVDKIDALEEKIQILDDEALKNM
                     TNVFKDRLSKGETLDDILPEAFAVVREVSKRILGMRQYRVQLIGGIVLHQGKIAEMRT
                     GEGKTLVGVAPVYLNALTGKGVHVITVNDYLAQRDKELMQPVYEFLGLTVGVIINGQQ
                     SAERKLQYQCDITYGTNNEYGFDYLRDNMVTQNSEKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGGGDEGTKLYELANAFIKTIKDEDFEMDRKDSTIALTASGLSKAEKFFGIEN
                     ITHIDNIEIFHHVNQSLRAHKLMDIDVEYVVRDNEILIVDEFTGRIMDGRRFTDGLHQ
                     AIEAKEGVKINDESKTMATVTFQNFFRLYSKLSGMTGTAKTEESEFEATYKINVVQIP
                     TNKPVIRADLHDKVFKTERAKYNAVVDEIIRINKTKQPVLVGTVSIEKSEKLSKLLKK
                     KGVKHEVLNAKQHDKEAEIISKAGKLGSVTIATNMAGRGTDISLGAGDKEEEEKVKEL
                     GGLYVIGTERHETRRIDNQLRGRSGRQGDPGTSRFFVSLEDEVIKLYGGKSIEKILKR
                     MNANEDDALESKALNRAIERAQKGIEGKNFEQRKNVLKYDDTINEQRKVIYDERNKVL
                     DGSDVEEEIQKMVMDIITEAAEKYLKKFRDYHGYFKYLYNTFMPADTLLIPDLDKKNV
                     EEIVNQTYQISKRVYDLKKMMIGIEELAQLEKRVLLQVVDTYWVDHIDAMDQLRQYIG
                     LKSYAQKDPFKEYAIEGYEMFEALNRNIRITTVQYLYKFN"
     gene            5367..7238
                     /locus_tag="KRH39_002653"
     CDS             5367..7238
                     /locus_tag="KRH39_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG8512545.1"
                     /translation="MNRKNLYVIMAAAMVSTSVAPSFAAKATQVKKQTITKKEATELV
                     SKVRELMAQRYTGGSQVGQPIYEIKVGETSSQLKIITNIDELEKLVNALGENKELLVT
                     IADKGHTTNSANEVVAEAIEKYENSADLSAEANSITEKAKTEATGIYGVATVEASYDS
                     TKDKLVITLKDKTDVVTSKTLQIGIGDEKVDLTANPVDIAGTNLDPSAEGFRVSKINK
                     LGVEGAKNIADIQLAEITIKNSDLNTVSPQDLYDGYRLTIRGNTIANSVGKSISDISS
                     KDSETGKYKFTVKYTDTSGKAIELTVESTNDKELKDVKDALNGNTKVNLIAGEDRYAT
                     AVAIAKETEYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKEI
                     IKKSPSAKIYIVGGESAVSNTARKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKEA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLTADAIKLMDGKEIGIVGGSNNVPSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKNDITNSQKNTLDKKLNLGAEVTQIGNGVELTVVQKIGKILGW"
     gene            7347..8054
                     /locus_tag="KRH39_002654"
     CDS             7347..8054
                     /locus_tag="KRH39_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG8512546.1"
                     /translation="MIEATLFFSPHQDDETLSMGSAIIEHVERSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESDFSKNRDEEFKNSCKAMGVKENNIHIENNRAH
                     DGELSKEKARDIMLKYLEKYPDAKVKTVTPYKALGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYNDFKKVNPNVRVWKVLSSQEEKLLNAMDAYKKWNPENGNYAIGYHSVK
                     SHFDELKSNKIQYVHAP"
     gene            8087..10219
                     /locus_tag="KRH39_002655"
     CDS             8087..10219
                     /locus_tag="KRH39_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG8512547.1"
                     /translation="MKISKRIMSLLTATFFIFALSINIADASSKESLVGSGRWETAIK
                     ISQNGWKDATNAILVNDNSIADALSATPFAKLKDAPILLTQSDKLDSRTKAELKRLGV
                     KNVYLIGGMNTLSTNIEKQLKSENMTFERISGNSRYETSLKLAERLDKEKNISDIVVV
                     NGEKGLADAVSVGSAAAQENMPIILSDPNNGIKVANSFISSKNIKKSYIIGGTYSVSS
                     SIEQSLPNVKRLSGNNRNETNAKVLEEFYKNTNLKNAYITKDGMKKQGDLIDSLAVGV
                     LAAKNSSPVVLVGNSLDSSQYDVLNTKSFDNIVQVGGNGNEDAVKDILDLQEETTYRV
                     KTADELTVALRKADANDVITFKPDDNKKITSSFSIETKKAITVELEGLYTQTITVDMP
                     NGELTNKGTLDGTVKIMDIKNGTFINEGDINDIDIYDPNGCKIENKSGGDIWVLTISS
                     SAKDVNIENKGDISKVTNNSTSVIIRNSGNIDTLSGINAPAISGNKPRVDDTDKDEKR
                     ASGIYPTSEPLSPARAGYISVKIFTKPEDTNYKIYYRVVSSRPSALYIGDRIDTSDWT
                     AVPSSVLPFTIKAENGYYIEAVEINSDTKKVSRWGKSDYIDDGFYLKSETAKGLSADI
                     DFDGDYVKLTATGADSGCTVYYRIVSKKPVSMNVGDKVTISDWDEITTNRVRIEANDV
                     KDKYIELVELDDSTNKVTRWGASDKVVE"
     gene            complement(10272..10667)
                     /locus_tag="KRH39_002656"
     CDS             complement(10272..10667)
                     /locus_tag="KRH39_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBG8512548.1"
                     /translation="MILKKYKETILYLFFGVFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIELRFLFKEFLFFVSFRLLSGIIEMIIMYIMIDLFF
                     VNDITVKVFTNIIVIVLNYLFSKMIIFRK"
     gene            10895..13306
                     /locus_tag="KRH39_002657"
     CDS             10895..13306
                     /locus_tag="KRH39_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBG8512549.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSVYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            13603..15180
                     /locus_tag="KRH39_002658"
     CDS             13603..15180
                     /locus_tag="KRH39_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBG8512550.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTTSVSR
                     AVEKSLPNAERLSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT         6281 a         1754 c         2848 g         4504 t
ORIGIN      
        1 tttgttggaa aaaacttcaa ttaattttgc ataaattatt gaatttatat tttatatagt
       61 ctatcataag agtgtgagaa atatttggat tatatgaaca tggaaattta atgtacataa
      121 ttaaagagat gtaattaata taatgttggg aggaatttaa gaaatgaata agaaaaattt
      181 agcaatggca atggcagctg ttactgttgt aggttctgct gctccaatat ttgctgatac
      241 tactgttaaa gaagaaggat atacggtagt acaagacaaa tatgaaaaat tacttaaaga
      301 attaaaagcg agaataaatg atggaactat tacatcagtt ggtgttgaat ttgatgggag
      361 tccaattgga actgttgctc ctgaaagtga tagtactgaa cataaaaaag cagcagctga
      421 taagttagaa acacttacta aaaaccaact taaaggttta ggtgatggaa agtatgtaga
      481 ttttaaaatt aattatggtg ctaaagatgc tataccagca cctactttat cagctaaaga
      541 catagatgct ttaaaagcaa aactagatga aatgaaaact aaacaactag ttgatgtagc
      601 taaagatggc caacctggta ttgcgaaagt agacggtagt gatgtaattg caggggatgc
      661 tccattgact atagcagatg ctgttacagt tacagaggga acagattctg gtaaaaaaac
      721 acttactata gctgctgcat ctactttaag taattttaaa tatggtgctc ttaaaactgg
      781 tggcgcagat gcttctagtg ttagtgccat aacactttct acttcaaact tagtaataaa
      841 agaaggcgaa gctaaaaaag taaatttgga ggagtcatta aaatttaact cagatactaa
      901 ggtaataagc ggatttgatg ttactacttt aaatgagact cctgcagatc caggatctag
      961 tactattgtt agagtaataa aagctgtaga aaaaacaata gatgtaagtt caagttcaac
     1021 tacaaaagct aaagatttag ctaaacaata tgtatttaaa gacggtgatg ctgagactcc
     1081 tggaaatttg agttatatgc ttaagtatat aaatgatgaa gataaaaaag tagctgtaaa
     1141 aaataatgat ggagattatg aagtaactat attcccagaa ggaaaaagat taaatacttt
     1201 atctgcatct tcagcaaaaa ctattctagg agaggcagat gctccagcaa aaatagtttt
     1261 aaaagcttca tcaacaaaga aattagcaga ttatatagat gatttaataa cttataataa
     1321 tagttattca aatgtacaaa ctgtagcagg tagtgataga atagaaactg ctatagaatt
     1381 aagccgtaaa tactataatt ctactgataa aaatgcttta tatggaaatc ctgtaaataa
     1441 tgttgtatta gtaggttctc aagctatagt tgatggatta gtagcatctc ctttagcagc
     1501 agaaaaggat gctcctttat tattaagttc taaagataaa ttagactcat caacaagagc
     1561 tgagataaaa agagttatgg acttaaattc ttcaacagga ataaaaaata ataaagaagt
     1621 tttcatagca ggtggtgtaa attctatatc taaagacgtt gaaaatgagc taaaagatat
     1681 gggacttaaa gtaactagat tatcaggtga tgatagatat gaaacttcat tagcgatagc
     1741 tgatgaaatt gatataaatg ataaagctta tgtagttggt ggaacagggt tagcagatgc
     1801 tatgagtatt gctccagttg catctcaaat aaaagatggt gaagctactc caatagtagt
     1861 tgtagatgga aaatctgaca agttatctaa agaagctgaa gatttcttag atgatgcaca
     1921 agtagatata ataggtggag aaaatagtgt ttctgctaaa atggaagatt atatagatga
     1981 tgctactgga aaatctccag aaagaatcag tggtgctgat agacaagcaa ctaatgctga
     2041 agttataaaa gaatactttg ataaagataa agtttcaaat tacttcttag caaaagatgg
     2101 ttcaactaaa gaggatcagt tagttgatgc attagcagca gcagcagtag ctggaaatta
     2161 cggttctaag cataatgcaa atggaaatat aactacagat gcttctcctg ctccaattat
     2221 tttagcaact gataatttat cagctgagca acatgtagct gtaagcaaaa cttctaattc
     2281 tgacgttgct aagaacttag tacaagtagg tcaaggaata gctgattcag ttgttagcaa
     2341 attaaaagat ttattagata tgtaatataa attttatata ttaaataaaa aggtttctct
     2401 tttgaggaac cttttttagt ttaaatataa gtttttataa ataaaaaaga ggctaattag
     2461 cctcttttta atgtgagaaa cacttaaaaa ataagatgta attatttatt taagtatcat
     2521 tattatacta taccttaaaa taaatgtcta ctattctata attttaaggt ataatagtaa
     2581 gtggaaaata aataaggagg taaattaaat atgtcagttt tagatgtaat actagacaaa
     2641 acagatgaat tagaaattaa aaaattaaat aatatagtag ataaaataga tgctttagaa
     2701 gaaaaaatac aaatattaga tgatgaagca ttaaaaaata tgacgaatgt gtttaaggat
     2761 agattaagta aaggagaaac cttagatgac atattgccag aagcttttgc tgttgtaaga
     2821 gaagtatcaa aaagaatatt aggtatgcgt caatatagag ttcaattaat tggtgggatt
     2881 gtattacatc aagggaaaat agcagagatg agaactggag aaggaaagac tctagtagga
     2941 gtagctccag tttacttaaa tgcattgact ggtaaaggtg tacatgttat cacagttaat
     3001 gattacttag ctcaacgtga taaagagctt atgcaaccag tatatgaatt tttgggatta
     3061 acagtaggag taattattaa tgggcaacag tcagcagaac gaaaattaca atatcaatgt
     3121 gatattactt atggtacaaa taatgagtat ggattcgatt atttaagaga taatatggta
     3181 acgcaaaact cagagaaagt tcaaagagaa ctaaactttg ctatagttga tgaggttgat
     3241 tcaatactta tagatgaagc tagaacacca cttataattg ctggtggagg agatgaaggt
     3301 actaagctgt atgaacttgc aaatgctttt ataaagacta taaaagatga agactttgaa
     3361 atggatagaa aggatagcac aatagcactt actgcaagtg gtcttagtaa agctgagaaa
     3421 ttttttggaa tagaaaatat tactcatata gataatatag aaatttttca ccatgtaaat
     3481 caatctctaa gagcacataa attaatggac atagatgttg aatatgttgt tagagataat
     3541 gagatactaa ttgtagatga atttacagga aggataatgg atggaagaag gtttactgat
     3601 ggtcttcatc aagctattga agctaaagaa ggtgtaaaga taaatgatga atcaaagacc
     3661 atggcaacag taacatttca aaatttcttt agattatact ctaaattatc aggaatgaca
     3721 ggtacagcaa agacagagga aagtgagttt gaagctacat ataagattaa tgttgttcag
     3781 ataccaacta ataaaccggt tataagagct gacttacatg ataaagtatt taaaacggaa
     3841 cgtgcaaagt acaatgctgt tgtagatgaa ataataagga taaataaaac caaacaacca
     3901 gtgcttgtag gaactgtttc aattgaaaaa tctgagaaat tatctaagct acttaaaaag
     3961 aaaggcgtaa aacacgaggt cttaaatgct aaacaacacg ataaggaagc tgaaataata
     4021 tctaaagctg gtaaattagg ttctgttaca attgctacta atatggcagg tagaggaact
     4081 gatatatcac ttggagctgg agataaagaa gaagaagaaa aggtgaaaga gttaggtgga
     4141 ttgtatgtta taggtactga aagacatgag acaagaagaa ttgataatca gcttagaggg
     4201 cgttcgggaa gacaaggtga ccctggaact tctagattct ttgtaagttt agaggatgaa
     4261 gtaataaaat tgtatggtgg aaaaagtata gaaaaaatat taaagagaat gaatgctaat
     4321 gaagatgacg cgttagaaag taaggctttg aatagagcaa tagaaagagc acagaaaggc
     4381 attgaaggta aaaattttga acaaagaaag aatgttctta agtacgatga tactattaat
     4441 gagcaaagaa aagtaatata tgatgaaaga aacaaggtat tggatggttc tgatgttgaa
     4501 gaagagatac aaaaaatggt tatggatatt attacggagg ctgctgaaaa gtacttaaaa
     4561 aaatttagag actatcatgg atattttaag tatttatata atacttttat gccagctgat
     4621 actctattaa ttccagactt agataaaaaa aatgttgaag aaatagtaaa tcaaacatat
     4681 cagatttcaa agcgtgttta tgatcttaag aagatgatga ttggaattga agaattggct
     4741 caattagaga aacgagtatt acttcaagtt gtggatactt attgggttga ccatatagat
     4801 gcaatggatc aactaagaca atatataggt cttaaatctt atgcacaaaa agatccattt
     4861 aaagagtatg ctatagaagg ttatgaaatg tttgaagctt taaataggaa tataagaata
     4921 acaacagtac aatatctata taaatttaac taaaaaagtg acccattggg tcacttttta
     4981 tatattaaga ttaaagtgaa atttatttta cagtacacac tcatatagat aaatatttat
     5041 aataatgaaa aagaggctga acgtcagcct cttttcctgt gtgagaaata cggaaaaaag
     5101 agatgcttga aatttctttc ttccatttaa tattatacta taaagagaat aaaaagtcca
     5161 tgagttatct ttacgattaa aaaaaaaaat tacaattttg ttacttttta gtatacaata
     5221 ataaatttaa attgactttg ttaattttta ttaataaaat atccaaatat tacaatatct
     5281 acagtttatt gaaataatgt gtaaattata ttataattgg taaataagga aaaatggtga
     5341 aaaccaaatt ttgtaggggg aaatatatga atagaaaaaa tctttatgta attatggcag
     5401 ctgcaatggt aagtacatca gtagctccaa gttttgctgc aaaagctaca caagtaaaga
     5461 aacaaacaat aactaaaaag gaagcaacag aattagtttc taaagttaga gaactaatgg
     5521 ctcagagata tacaggaggt tctcaagttg gacaaccaat atatgaaata aaagttggtg
     5581 aaacttcatc acaattaaaa ataataacta atatagacga attagagaaa ttagtaaatg
     5641 ctttaggaga aaataaagag cttcttgtga ctatagcaga taaaggacat acaacaaata
     5701 gtgcaaatga agtggttgct gaagcaattg aaaaatatga aaattcagca gacctttcag
     5761 ctgaagctaa ttcaataaca gaaaaagcta aaactgaagc tactgggatt tatggagtgg
     5821 caactgtaga agcttcatat gacagtacaa aagataagtt agtaataact ttaaaagata
     5881 agactgatgt agtaacttct aaaacgctac aaataggtat tggtgatgaa aaggttgatt
     5941 taacagcaaa tccagttgat atagcaggaa ccaatttaga cccttctgca gaaggattta
     6001 gagtaagtaa aataaataaa ttaggtgtag aaggagctaa aaatattgct gatattcaat
     6061 tagctgaaat aactataaaa aatagtgact taaatacagt ttcacctcaa gatttatatg
     6121 atggatatag attgactatt cgaggtaata caatagcaaa tagtgtagga aaatcaatta
     6181 gtgatatttc atcaaaagat tcagagacag gaaagtataa atttactgtt aaatatactg
     6241 atacatctgg aaaagcgata gaacttactg tagaaagtac taatgataaa gagttaaaag
     6301 atgttaaaga tgccttaaat ggaaatacaa aagtcaattt aatagctggg gaagacaggt
     6361 atgcaactgc tgtagctata gcaaaagaaa cagaatatac tgacaatata gttatagtta
     6421 attcaaataa attagttgat ggattagcag caacaccact agctcaatct aaaaaagctc
     6481 ctatattgtt ggcgtctgat aatgaaatac caaaagtaac tttagattat ataaaggaaa
     6541 taattaaaaa aagtccatca gctaaaatat atatagtagg tggagaatca gcagtatcaa
     6601 atacagctag aaagcaatta gaatcagtga ctaagaatgt tgaaagattg gctggagatg
     6661 atagacatac aacttcagta gcagtagcaa aagctatggg ttcttttaaa gaagcatttg
     6721 tagtaggtgc aaaaggggaa gctgatgcta tgagtatagc tgctaaagct gctgaactta
     6781 aggctcctat aatagtaaat ggttggaatg atcttacagc agatgctatc aagttgatgg
     6841 atggaaaaga gattggtata gttggtggtt ctaataatgt acctagccaa attgaaaatc
     6901 aacttgctga tattgataaa gatagaaagg ttcaaagagt tgaaggtgaa acaagacacg
     6961 atactaatgc taaagttata gaaacatatt atggcaaatt agataaacta tatatagcaa
     7021 aagatggata tggaaataat ggtatgttag tagatgcatt ggcagcaggt cctctagcag
     7081 caggaaaagg accaattctt ttagctaaaa atgatataac aaactcacaa aagaatactt
     7141 tagataaaaa actaaatctt ggtgcagaag taactcaaat aggtaatggg gttgaattga
     7201 ctgtagttca aaaaataggc aaaatactag gatggtaata atcaataata tagaaaagga
     7261 ttccttatta tttataggaa tctttttcta tcgacataat attcattaaa gtataacatg
     7321 ctcttgacag ataggaggat atgatagtga tagaagcaac attgtttttt tccccacacc
     7381 aagatgatga aacattaagt atgggtagtg caataattga acatgtagaa agaagcgata
     7441 cacatgttat attatgtact gatggcagta aatcaataat aagaaaagta ttagatgatg
     7501 gagggtcatg ttcttatcat attaaagatg tacacaagta ctctttatca gaaagtgatt
     7561 tttcaaaaaa tagagatgaa gagtttaaaa atagttgtaa agcaatggga gttaaagaaa
     7621 ataatataca tatagaaaat aatagagctc atgatggaga attgagtaag gaaaaagcaa
     7681 gagatataat gctaaaatat ttagaaaaat atcctgatgc taaagtgaag accgttacac
     7741 catataaagc tcttggaata catgaagacc atagagcatt aggtgaagct gcattagaat
     7801 tgtatagaga aggaaaaata aaagatttaa gattttatgt agagccatac gattataatg
     7861 attttaaaaa agttaatcca aatgttagag tatggaaggt tttgagtagt caagaagaga
     7921 aattattaaa tgctatggat gcatacaaga aatggaatcc tgaaaatggt aactatgcaa
     7981 ttggatatca ttctgtaaaa tctcactttg atgaattaaa atccaataaa atccaatatg
     8041 tacatgctcc ataaacatta atataaaaac taggaggata tagttaatga aaatatcaaa
     8101 gagaataatg tcgttgttga cagcaacttt ttttatcttt gcattatcta tcaatatagc
     8161 tgatgcttca tcaaaagaga gtctagtggg ctctggtaga tgggaaactg caataaaaat
     8221 tagtcagaat ggatggaaag atgctactaa tgctatacta gtaaatgata attctatagc
     8281 tgatgcatta tctgcaactc catttgctaa gttaaaagat gcacctattt tattaactca
     8341 aagtgataaa ttggatagta gaactaaagc agaattaaaa agactaggtg taaaaaatgt
     8401 atatttaatt ggaggtatga atactctaag caccaatatt gaaaaacaat taaaatcaga
     8461 aaatatgact tttgagagga tttcaggtaa tagtagatat gaaacctctt taaaattagc
     8521 tgaaagatta gataaagaga agaatatttc tgatatagtt gtagttaatg gagagaaagg
     8581 gctagcagat gcagtaagtg tgggttctgc tgcagcacaa gagaatatgc caattatact
     8641 ttcagatcct aacaatggaa taaaagttgc aaatagcttt attagtagta aaaatataaa
     8701 gaaatcgtat ataattggtg gtacatattc tgtatctagt tctattgaac agagtttacc
     8761 taatgtaaaa agattatctg gaaacaatag aaatgaaact aatgcaaagg ttctagaaga
     8821 attttataaa aatacaaact taaaaaatgc ttatataact aaagatggta tgaaaaaaca
     8881 gggggatttg atagattctc tagctgtagg tgtattggca gctaaaaata gctctccagt
     8941 tgtattagta ggaaattcac ttgactctag ccaatatgat gtgttaaata ctaaaagctt
     9001 tgataatatt gtgcaagttg gtggaaatgg aaatgaagat gctgtaaagg atatccttga
     9061 tttacaagaa gaaactacat atagagttaa gacagcagat gaattgactg ttgctctaag
     9121 aaaagcagat gctaatgatg ttataacatt taaacctgat gataataaaa aaattactag
     9181 ttcatttagt atagaaacaa agaaagctat aactgtagag cttgaaggat tgtatacaca
     9241 aacaataact gtagatatgc caaatggaga attaactaat aagggtacat tagatggaac
     9301 tgtgaaaata atggatataa aaaatggcac atttatcaat gaaggtgata taaatgatat
     9361 tgacatttat gatccaaatg gatgtaaaat agagaataaa agtggtggag atatatgggt
     9421 tttaactata tctagtagtg ctaaagatgt aaatatagaa aataaaggtg atataagtaa
     9481 ggttacaaat aattctacta gcgttataat aagaaattca ggaaatattg atactttaag
     9541 tggaattaat gcaccagcaa taagtggaaa taaacctaga gttgatgata ccgataagga
     9601 tgaaaaaaga gcaagtggaa tatatcctac ttcagaacct ttatcacctg caagagctgg
     9661 ttatataagt gtaaaaatat tcactaagcc tgaagataca aattataaaa tttattatag
     9721 agtagtaagc agtagaccat ctgcattata tatcggagat agaatagata caagtgactg
     9781 gactgctgta ccatcttctg ttttaccatt tactattaaa gcagaaaatg gatattatat
     9841 agaagctgtt gaaataaatt ctgatactaa gaaagtatct agatggggga aatctgacta
     9901 tatagatgat ggattttatt taaaatcaga aactgcaaaa ggattaagtg cagatataga
     9961 ttttgatgga gattatgtta aattaacagc aacaggtgct gattctggat gtacagttta
    10021 ttatagaata gtatcaaaaa agccagtatc aatgaatgtt ggagataaag taactatatc
    10081 tgattgggat gaaattacta ctaatagagt gaggatagaa gcaaatgatg taaaggataa
    10141 atatatagaa ctagttgaat tggatgattc aactaataaa gtaactagat ggggagcaag
    10201 tgataaggta gtagaatagg tatctaatgt gaaacataaa aacagactga ttaataatgg
    10261 gcagtctgtt tttatttcct aaaaattatc attttactaa ataaataatt taaaactata
    10321 acaattatat tagtaaatac ttttacagta atatcattta caaaaaataa atcaatcatt
    10381 atatacataa taatcatttc tataatacca gaaagtagcc taaaactgac aaaaaacaaa
    10441 aactccttaa acaaaaatct tagttcaatt cttttacttt caaacacaaa aaacttatta
    10501 gtaacataag caaataaaac agcaagtatc catgccaaag catttgcaac cataaaatta
    10561 aataaaataa ctctagtaaa aaataaatac gaaactatat ttacaagagt agtaaatact
    10621 ccaaaaaata aatataatat tgtctcttta tactttttta aaatcaaata taatcctcct
    10681 aagctaattt aatataaata tacttaaaga tactaacaaa cattagtata gcacatctat
    10741 taataaatca atatatctta aaatattata aaaaataaaa ctataatatt ctataattaa
    10801 tatgtactca taatccatat tttatcaaaa taaaatatga ccttaattta gaaatctgat
    10861 ataataaaaa ttaaatacat ataagggggt aaacatgaga aagtacaaat caaaaaaatt
    10921 gtcaaagcta ctagcattgt tgacagtttg ctttttaata gtgtcaacaa tacctgtctc
    10981 agcagaaaac cataaaactc tagatggagt agaaactgca gagtattctg aaagctatct
    11041 tcaataccta gaagatgtca aaaatggaga cacagcaaaa tataatggag taataccttt
    11101 tccacatgaa atggaaggta caacacttcg aaataagggt agaagtagtc ttccatcagt
    11161 atataaatca agtgtagcat acaacccaat gaatcttggt cttactacac cagcaaaaaa
    11221 tcaaggaagt cttaatacat gctggtcttt ttcaggtatg tcaactttag aagcatatct
    11281 taaattaaag ggatatggaa cgtatgactt atcagaagaa catttaagat ggtgggcaac
    11341 tggtggaaaa tatggatgga acttagatga tatgtcaggg agttcaaatg taacagctat
    11401 agggtatctg acagcatggg caggtcctaa gttagaaaaa gatataccat ataatcttaa
    11461 atctgaggca caaggtgcaa ctaaaccttc aaatatggat actgcaccta ctcaatttaa
    11521 tgtaacagat gttgttcgtc ttaataaaga taaggaaact gtaaagaatg ctataatgca
    11581 atatggttca gtgacatcag gttatgcaca ttattcaaca tattttaata aagatgaaac
    11641 agcatataac tgtacaaata agagagctcc attaaatcac gctgtagcga tagtaggatg
    11701 ggatgataat tattcaaaag ataactttgc atctgatgta aaaccagaat caaatggagc
    11761 atggttagta aaaagcagtt ggggagagtt taattctatg aaaggattct tctggatttc
    11821 ttatgaagat aaaactctac taacagatac agataactat gcaatgaaat cagtatcaaa
    11881 accagatagt gataaaaaaa tgtaccaact tgaatatgct ggtcttagca agataatgtc
    11941 aaataaagta acagcagcaa atgtatttga ttttagcaga gattctgaaa aacttgactc
    12001 tgttatgttt gaaacagatt ctgtaggagc aaaatatgaa gtatattatg caccagtagt
    12061 aaatggagtt cctcaaaaca attcaatgac aaaacttgca agtggaacag tatcatattc
    12121 tggatacata aatgtaccta ctaattctta cagcttacca aaaggtaaag gagcaatagt
    12181 agtagttata gacaacacag caaatcctaa tagagaaaaa tcaactttag catatgaaac
    12241 tgatatagat ggatactatc tatatgaggc taaagcaaac ttaggtgaaa gctacatact
    12301 tcaaaacaat aaatttgaag atataaatac atatagtgaa ttttctcctt gtaactttgt
    12361 tataaaagct ataacaaaaa catcttctgg acaagctact tcaggagaat ctttaactgg
    12421 agcagataga tatgaaacag cagttaaagt tagtcaaaaa ggatggactt cttcacaaaa
    12481 tgcagtatta gtaaatggag acgcaatagt agatgcttta acagctacac catttacagc
    12541 agcaatcgac tctccaattc ttttaacagg aaaagataac ttagattcaa aaactaaggc
    12601 agagttacaa agattaggaa ctaaaaaagt ttatctaata ggtggagaaa attctttaag
    12661 caagaatgta caaactcaac ttagtaatat gggtatatca gtagaaagaa tttcaggtag
    12721 tgatagatac aagactagta tatctctagc tcaaaagtta aatagtataa aatctgtttc
    12781 acaagttgca gtggcaaatg gtgtaaatgg acttgcagat gcaataagtg ttggtgcagc
    12841 agctgctgat aacaatatgc caataatact tactaatgaa aagagtgagt tgcaaggtgc
    12901 tgatgagttt ttaaattcat caaaaataac taagtcttat ataattggtg gaacagctac
    12961 tttatcatca aatttagaaa gtaagctttc aaatccaaca agacttgcag gaagtaatag
    13021 aaatgaaact aatgctaaga taatagataa attctaccct agttcagatt tgaaatatgc
    13081 ttttgttgtt aaagatggtt caaagagtca aggagacttg atagatggat tagcagtagg
    13141 agcattaggt gctaaaactg attcgccagt agttctagtt ggaaataagt tagatgaaag
    13201 tcaaaaaaat gtacttaagt ctaagaaaat agaaactcct attagagttg gtggaaatgg
    13261 aaatgaaagt gcttttaatg aactaaatac tcttttagga aaatagtgaa tggaaagata
    13321 gtaaggatat acattttatt attaaaaaat attcaataga agtttataaa tataaaataa
    13381 caaaaagggg tgtctcaaaa tgaacttttt agttcgtgag gcactctttt ttgttatttt
    13441 atatttttat catttaaaga atgaaaaata ggcttgtcta tacttttaga tagttctctt
    13501 attattgatg aaaaatgtaa taattttaaa ctgtaaaata gtgtaaagtt attttataat
    13561 ataaatatag taagatttta aacaaaataa gggggaataa taatgaaagc accaaaaact
    13621 attttaacaa tactgacaat agcgcttact ttaagtagca tttctataat accatcatat
    13681 gcacttacag aggaaaaatt aataggtaat ggtagatatg aaactgctgt aaagataagt
    13741 caaaaggcat acagttctag ttctaatgta gtgctagtta atgacaattc attagctgat
    13801 gcactttcag ctacaccatt tgcaaaggct aaaggagcac caatactttt gacagaaagt
    13861 gataagctag atgatagaac tgaaaaagag ataaaacgtc taggagctaa agatatttat
    13921 ttgataggag gtactgctgt acttaataaa gatatagaga acaagttaaa aggtaatgga
    13981 cttaatgtag aaagaattaa tggtaaagat agatatgaaa cttcactgat tttggctaat
    14041 aagcttaagg atataaaaga tattaaggaa gttgctgtag taaatggtga aaaaggattg
    14101 agtgatgctg ttagtgttgg agcaccagca gctcaaaata agatgcctat aattttgtct
    14161 agtcctaagg atggagtaga agcatttgat aaatttataa gggatgagaa agttataaaa
    14221 gcatatgtaa ttggaggtac aacttcagtt tcaagagctg tagaaaagag tcttcctaat
    14281 gcagaaagat tgagtgggaa agatagaaat gagactaatg ctaaggtcat agaaaagttt
    14341 tatactgata caaatttaag taatttatat gtaactaagg atggtagtaa aaatgagaat
    14401 caattaatag actcgttagc tgtcggagta ttggctgcaa aaaatgagtc tcctgttgtt
    14461 ttggttggta ataaattaaa tacaaaacaa agagatatac ttagtactaa aaaattaaat
    14521 actataacac aggtaggtgg aaatggaaat gaagaagctt ttgatgaaat taaaagccta
    14581 caagaaaaaa ctgtgtttga agcaaaaact gttgaggaat taactgatat gataaatata
    14641 gcaagtccta atgatattat aaactttaaa ccaaaagaga atactgtaaa tgaagctttt
    14701 agaatggtaa caaataaacc aataactgtg aatataaagg gtgattgttc aaagactctt
    14761 acagtagata tgcctaatgg tgaagtgaat aactatgcta ctttggtaaa tgttatagtt
    14821 agaaatattg gagaaggtgg atttaataac catgatacaa tcactatatt gtcagttcgt
    14881 gacaaaaatg gtagagttat agaaaatact agaaattcag atatagatac gttgatgata
    14941 ttagcttctg ctaatgatac aaaattgatt aatgatggat atataggaaa acttatagat
    15001 aattcatcta atagtgatat aactaataat ggtactatag ataaaaaagt taatcaggtt
    15061 gaggatttag aggctaaagt agattcaatt gaaaaagcta tagattctat aagtcaaaaa
    15121 gtcaataaaa tacaagatat actagataag cttggatttc taaagaaatt tcttagctaa
    15181 gaatatgtat atttaagtga agtgtgtgtt gtaattgttc taaagttata tagttaaaca
    15241 ggtttatata caaacagact tatagataaa tatgtagttc aatatatatt tgaataagct
    15301 ttggggaggt aacatgggga ttggaattga atgtaacaaa aaaataaata aaagtattga
    15361 caaaactaga ataaagaaaa ataatct
//
LOCUS       DAEORZ010000099        15295 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEORZ010000099 DAEORZ010000000
VERSION     DAEORZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.099"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            363..1898
                     /locus_tag="KRH39_002659"
     CDS             363..1898
                     /locus_tag="KRH39_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG8512551.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKWTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            1914..2675
                     /locus_tag="KRH39_002660"
     CDS             1914..2675
                     /locus_tag="KRH39_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512552.1"
                     /translation="MIPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            3013..3882
                     /locus_tag="KRH39_002661"
     CDS             3013..3882
                     /locus_tag="KRH39_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8512553.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEISYSKYHLHRMF
                     TSVVGFSIHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            4211..4462
                     /locus_tag="KRH39_002662"
     CDS             4211..4462
                     /locus_tag="KRH39_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG8512554.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDYIITVNAHSYTIITAHKEKK"
     gene            4574..5353
                     /locus_tag="KRH39_002663"
     CDS             4574..5353
                     /locus_tag="KRH39_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG8512555.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(5488..6138)
                     /locus_tag="KRH39_002664"
     CDS             complement(5488..6138)
                     /locus_tag="KRH39_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512556.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSISK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(6135..6638)
                     /locus_tag="KRH39_002665"
     CDS             complement(6135..6638)
                     /locus_tag="KRH39_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8512557.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDNNIKILPNTKEIHKCIGCFGCWI
                     KTPTECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(6687..7202)
                     /locus_tag="KRH39_002666"
     CDS             complement(6687..7202)
                     /locus_tag="KRH39_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8512558.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFIEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKIDVEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKNLLKNL"
     gene            7518..8009
                     /locus_tag="KRH39_002667"
     CDS             7518..8009
                     /locus_tag="KRH39_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8512559.1"
                     /translation="MRIIDIKGENMRELLKWVSIINRYNTNYIDRKLADIGINNSQYF
                     YVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVKDKRTWHLYPTE
                     KSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMNEEAKLEAKENG
                     RKI"
     gene            7993..9396
                     /locus_tag="KRH39_002668"
     CDS             7993..9396
                     /locus_tag="KRH39_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8512560.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(9643..9954)
                     /locus_tag="KRH39_002669"
     CDS             complement(9643..9954)
                     /locus_tag="KRH39_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512561.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIILIGLNPILSII
                     STIDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(10010..10603)
                     /locus_tag="KRH39_002670"
     CDS             complement(10010..10603)
                     /locus_tag="KRH39_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG8512562.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLENLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
     gene            11160..11390
                     /locus_tag="KRH39_002671"
     CDS             11160..11390
                     /locus_tag="KRH39_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512563.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFCFCKDLLNHKIKYVLIRD
                     IFVNRTTYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            11447..12097
                     /locus_tag="KRH39_002672"
     CDS             11447..12097
                     /locus_tag="KRH39_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG8512564.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNKIVTK"
     gene            12301..12972
                     /locus_tag="KRH39_002673"
     CDS             12301..12972
                     /locus_tag="KRH39_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8512565.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            12969..14099
                     /locus_tag="KRH39_002674"
     CDS             12969..14099
                     /locus_tag="KRH39_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8512566.1"
                     /translation="MNSLRKIVEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLIVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQKALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLIINAKKHVVYDGNLDLSITCENGLLLFEIYNDGRIIEQEEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILSMQGLEYGVKNRDKGVEFYFMIPIDE"
     gene            14542..>15295
                     /locus_tag="KRH39_002675"
     CDS             14542..>15295
                     /locus_tag="KRH39_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512567.1"
                     /translation="MIKVEELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFTNKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMAIDKPKNL
                     KMKNSNNKVEVEFLYNGNRDIEIVDIQELESGI"
BASE COUNT         5734 a         1701 c         2529 g         5331 t
ORIGIN      
        1 tataaaatga taattaaact taacagtaat aaataatgtg agaaatattc aaatttaatt
       61 aaataaagtg tcagaaatgt gtttaaattt tagtatttta acggttctga ccttttttct
      121 tatttttata gagaatatcg aggatggagt ctctaatagt tacttttttg tgtctacttg
      181 caagtttgat taaaaaatgg ctatacacac ttgtgacttc agtcatggga ggttcagatt
      241 ataatcttaa tatataattt gtgaaataat ttgatgacga tttgctgatg tataaacaat
      301 aaagattaac tacattaaaa tttaaatctt gttaagtaaa acttataaaa ggagagagat
      361 ggatgataaa tgatattcta accttattat ctgaaagaag tgattttttt ataaagctat
      421 ccatagaaca tttgatgatt tctattattt ctataataat tgcgattgtt ttgggtggta
      481 taattggtat tattgtaagt gaatttcata attcttctaa acctatattg ggaactatca
      541 attttctata caccattcca tctatttcta tgctaggatt tctgatacca ttttccggaa
      601 ttggaaatac tactgctgtt attgcactta ccatttatgc attattgcca atggttagaa
      661 atacatatac tggaattact aatgtggata aaaatattct tgaagcagct aggggaatgg
      721 gaagtacaga ttttcagata ttgtataaaa taaaacttcc gttagcccta cctgttatta
      781 tgtctggtat tagaaatatg gtgattatga caattgcatt agctggtatc gcttcattta
      841 tcggtgctgg aggtctaggt gtagccatat atagaggaat cacgacaaat aacacaataa
      901 tgacaattac tggaagtata cttattgctc tattagcttt aatatttgat tttattttag
      961 ggctggtaga aaaatttttt caacgaaaaa gaaaaaagaa aaataggaaa aaagtttttg
     1021 ccattctagg aagccttatt cttatagcaa ctatctttac gtcttattta tttaatcctt
     1081 caaagactca aactattcat attgctacta aacctatgac tgaacaatat attcttggtg
     1141 agatgttgga tattttaatt gaaaaaaaca caggtctaaa tgtggaaatt acacaaggtg
     1201 taggaggggg aacatctaat atccagccag ctatggagaa tggagaattt gacatttatc
     1261 cagagtatac aggtacaggc tggaatatgg tacttaagaa aaatgaatta tatactgaaa
     1321 gtatgtttag taaactacag tcggaataca aaactaaact taaaatgcaa tggactggta
     1381 tgtatggttt ttctaatagc tatgcattag cagttagaaa agaagttgca gataaataca
     1441 atttaaaaaa tataacggac ttaaaagatg tatcttctaa atggacattt ggagctgaat
     1501 atgactttta tgagagagag gatggatata ctccattgtg taatacatat ggactgaact
     1561 ttaaagatac agttgattta gatattggat taaaatacca agctatcaat gaaggtaaga
     1621 ttgacgtaat gcctatattt acaactgatg gtcagtatag tgtttcagat gttgtagttc
     1681 taaaagatga taaaaatttc tacccatctt atcaatgtgg aaatgttgtt cgtttggaag
     1741 tattagagga aaatccagaa ctatcaaagg tatttaagat ggttggagat attttgactg
     1801 ataaagaaat ggctaaaatg aattatgatg tagaaaacaa caatttagaa cctagagaag
     1861 ttgctcgtga gtttctgaat aagaaaggtc ttttataaat tggaggaaat ataatgatac
     1921 ctattataca atttaaaaat attaaaaaac aatataatga taaaactata atagataatc
     1981 ttaatttaga tatagaaaaa ggagagtttt tgactgtaat tggttcgtct ggttctggta
     2041 agacaacact cttaaaaatg attaatggtc tgattttgcc agatggagga aatattttga
     2101 taaaccaaac agatatcaaa aacgaggact taattaaatt aagaagaaga ataggatatt
     2161 gtgtacaagg aagtgttctt tttcctcata tgactgttga agaaaatatt tcatatgtac
     2221 caaatctcct aagtaaaaag aataaattag aagtaaaaag tgctgtaaac aagtggatgg
     2281 aaatagtggg attgccaaat gatatgaaag tgcgttaccc atcagaattg tcaggtggtc
     2341 agcaacaacg tgtagggatt gcaagagctt tagcttcttc tccagaaatt ttattaatgg
     2401 atgaaccttt tggtgcagtt gatgagatta cacgaaagca attacaaaaa gagataaaag
     2461 aaattcataa aaaaacagga gttacgatta tctttattac gcatgatatt tatgaagctt
     2521 tgattttggg aacaaagact ttggtattaa atcatggagt tgttcaacag tatgatactc
     2581 ctgagaatat tttaaatact cctgctaacc aatttgtaga ccagttattg aatataagaa
     2641 aatctataat agatgatgat aatttaaaag aataaattta aatttaaaat caaattgttg
     2701 tgataaaaac tattgattta gagctaactt caagttgtat acttgaagtg gaaaagttag
     2761 attaaggtat agaagggaat ttagcagttg gctgtagaca aggtaactgc tgaaggtgat
     2821 ttcaataaat ggctggaacc tgtatctgtt gaagattata ataaattgaa ataagaagct
     2881 caatatacag agtagatagg cacttcaaat agaagtgtct tttgtgtgcc ataaaacaaa
     2941 tcaaaatatt agcaaagaat gtgaaaaaca aaataaaata atatgctatc tttaaaatag
     3001 gaggggtgct atatggataa caatttgatt accattgaaa aaactttaga ttatatagaa
     3061 aaacatcttt tagatgagat gttagattta aatgtaattt ctaaagagat aagctattca
     3121 aagtaccatt tacatagaat gtttacatca gttgtcggat ttagtataca taattatata
     3181 caaagacgta gactgactga agcagcgaga atgctcattt ttacagaaaa accaattatt
     3241 gaaattgctt taatctcggg gtatgacaca caacgctctt tttcaagagg gtttaaaaaa
     3301 atgtttaaac gtagtcctaa tttttttaga agacatcaag attttttacc actacaatta
     3361 aaatttgata ttcataatag aaaaaaatta cgtggagata tggttttaga tgttaaaaaa
     3421 gttaattgtg cagatataaa tattgttggc tattctggta aagtaagaaa aggatttcat
     3481 attattggaa aatgctggag ggatttgcat aaaaataaag agatgataga aaataaggta
     3541 gatttaggtt ttttaattgg agttagtgat tatagcaatt ttaaagagat tgagaatact
     3601 cctacattta catacattgc aggtgctcaa gtagctactt ttggcaaatt acctaatgga
     3661 atggaaaaat tcgtattacc gaaaagtaat tatattgttt ttacttttat tgggaaaaat
     3721 gaggatagta tgcaaaatgt tgtagaatat atctatcaag aatggttccc taattcaagt
     3781 tgccagttta atgaaaataa tttatatgac tttgtaaaat atggagaaaa aactgatgag
     3841 aatggtttga gtgaaattca gttttgggtt ccaatcattt aaaagaaggt attatgtata
     3901 aagtagatgg atttatatag gaaaagtttt acagcagtat gtatttataa ataatttata
     3961 gtttgtagga aagaaataat tttactataa gattatgata aaatacacaa tactatgaca
     4021 aaatagtgtt aaataataga tttaattttg cataggaaaa gttctataat ggtgtgtatt
     4081 tataaataat ttataatctg taggaaagaa atagttttac tataagatta tgataaaata
     4141 cacaatacta tgataaaata gtactaaata atagatttaa ttttgcaaag gataaggtgt
     4201 tttaataaaa atgaatgtag aaaacgaatt acttaaaaac ctagatagat tgcatacaac
     4261 agagttaggc gttgtaagaa ttaagaaaaa tctttctttg gagacaaatg atgtagtgaa
     4321 ctggtgtaaa actaaaatag aatctccaaa tgccattatt aatagaaagg gaaaaaactg
     4381 gtatataagt gtatatgatt atattataac agtaaacgct cacagttata caattattac
     4441 agctcataaa gaaaagaaat gaattgcaaa atacaaaggt caaaagcatt ttccactgtt
     4501 tgtagtacct atgaagaagt atataaaata atattacttt tgaataataa tttaaatttt
     4561 ggaggttgaa aaaatgaatg atactgtaaa agaaaaattg gcagagtcac taacttctga
     4621 tactactgat attttacctt atttgcctta tttattacaa gatttttggg aattaggaag
     4681 ttcaccagaa caaatgttac aattatttag aaagcatata ccttttaatg aagatacaaa
     4741 agttttggat ttagcttgtg gaaaaggtgc tgtatctgtg aagcttgcaa aagagttgaa
     4801 tatacatatc aaaggtattg atttgatacc tgaatttatt gattatgcga gaagaaaggc
     4861 tattgaatat aaggttgatg ctttgtgtga ttttgaaatg aatgatgcta atagttctgt
     4921 aaaaattgag catgattatg attgtgtgat ttttggagct gtaggggata ttttaggtaa
     4981 ttataaagaa actataagta agctattacc tacaataaaa agtaaggggt tcattttaat
     5041 tgatgatgca tatgtagtag ataaatctac aaatgaacag cttaattttg agagggtgta
     5101 tcccacttac aatgagtggt gtatattgtt caaagaatta ggtctgaaac taattgaatg
     5161 tataccatgt ttagaacttg aaaatgatgt tgaagaggaa atgaactgga ttataactcg
     5221 tgcaaatgag ctaattaaaa aatatccttc tcaaaaagat atgtttgaac aatatgttat
     5281 tagccaaaaa aatgaatata ctgatttaca gcaggacctt gttggagcaa cttggatttt
     5341 acaaaaagaa taagataaat attagactag ataatcaatg ttgtagtgaa ttatcccatg
     5401 tctagtgaat ataaataaca taaaaaaccg tcaagatgaa tagtttattc gtgcttgacg
     5461 gtttttagta tttgttgaag taaaatttta tttctttagt atatcacttt tagtcaatcc
     5521 atttgattta gcttgagtat tccattgatg attccccata agtttatata ggaatctagg
     5581 gaagttaggt gaaataaaaa tagtactttt gttggaatca tctatatgta tattatcact
     5641 aagtatattt tttgaaagtt cttttaatgc aattcctaaa tcttttttag gtcctttacc
     5701 caaaggtacg gattctagtg aaccaaacat ctccccagct ccaattccaa taccttgaac
     5761 ccatgtaaag ttgatttttt tacaccaatg ctctatcatt tgaattgcca aatgattatg
     5821 ttcaccttca ttgaatccac agttaactat tgcatacact ggaataggtt ttttattttg
     5881 tgtttttaaa aatttttcac atttctctaa aaaatgaagt aaatgagatg gaataccatc
     5941 tacataaaga ggagatgcga ttattaatct atcacaatta agtatggcat taagagcgtc
     6001 aggatactta gatatgctat ctactttaaa tgtaaaaact ttattatcat tttgaataag
     6061 ttgttttaat tcagataaaa aatactgtga attattttta tttacttttg gactaccatt
     6121 tatcattact atattcatat ggattcacct cttatatctt gtatattttc atagaaacaa
     6181 acagagtgtt cttttatatt gaaatttata gcatttccat ttactaattt cgtagcagtt
     6241 tctttttcag cttctgtaat acaatctcca tagaatatag cttttaaatt tatctgtttg
     6301 tcatatctgc ttttatgacg catcatatta tcttttatta caaagtatgg aagaatataa
     6361 ttaatgcttt tatcaagtac gtttttgaca aaagggctaa accctccata acagcatctg
     6421 cttattataa taattccatc agattcattt ataagtttac ccatgttgct gtaataatct
     6481 tttacaacac attcagtagg cgttttaatc cagcagccaa aacaaccaat acatttgtgg
     6541 atttcttttg tgtttggaag tatttttata ttgttatcaa gcttgggtaa taacttttct
     6601 aattctttgt cagttaaatc atgtattaaa agcctcataa attccatctc cttttattat
     6661 gtatgtttaa ttctaaaaat tatatcttat agatttttca gtaaattttt acaccaatta
     6721 ataacaaaat tataatagtc aactccataa atcattgttg catacatata tgtatattgg
     6781 tcaacacaat gattttcttc tacatcaatt ttttgtttac ttaatttatt tagaagtaat
     6841 tctacttcag ttattaacat ttttaagttt gtttcaatta tttctttggg tagatacttg
     6901 taaaagaata tattaacaag gtggtcttgc tttgttttag aaaaatttat aggtttcttt
     6961 aaccattcaa taaatacttc tttaccagta ttagttatgg aatatagttt ttttaatttg
     7021 ctaccatcta taacttcgtg atagtgtata tatccttttg tttctaatct tttaagtgca
     7081 ggatagatac ttccaaagct ggcgtcaaaa aagtacgagg tactctctga cattatttgt
     7141 tttaagtcgt atccacttaa ttctttttcc attaaaaatc ctaaaataat gtattctaac
     7201 atattgcctc ctagtatcaa ttagatatat atcaattcga tatacttatt attgcagata
     7261 ataaatatta cgtcaagatg ctttttaaaa taagctaaaa agaattagaa attattaata
     7321 gatatgcaaa aaaattaata taaaaggtaa ttaaatatca atgaagactt aaaaatagtt
     7381 aaatcaaagt aagagtctaa tatataaaat ttatagtaac tttatggttt aaatataaac
     7441 ttgtgtgcta ttgacaaagt ataaaattag cagtaaaata attgcatgag caaatagatt
     7501 attgctagcg caaaaaaata aggataatag atattaaagg agagaatatg agagagctat
     7561 taaaatgggt ttccattata aatagataca atacaaatta tattgacagg aaattggcgg
     7621 atattggaat caacaatagc caatattttt atgtattaca catatgtaat aatccaggta
     7681 ttacacagga cactatattt gagaaaattc ttgtgaatcc tagtaatata acaagagctt
     7741 tggcaaattt agaaaaagaa ggctttataa caagagaacc aggtgtaaag gataaaagaa
     7801 catggcattt atatcctaca gagaaatctt tagaatgtta tgatgaaata ctaaaaataa
     7861 caaatgggta tgttgaggag ttgttgagtt catttgagaa agaagagcaa gagttgttta
     7921 tgtcaatgtt aaagaaagtt gcatttttgg ctattgatat gaatgaagaa gcaaaattgg
     7981 aggcgaagga aaatggaaga aaaatataag gagccattgg aatatgaaaa ggtttcaaag
     8041 ttattaacca agtacgcaat tcctagtatt atagcaatgt tagtaagtgc tttatataac
     8101 attgttgacc aaatatttat tgggcaaggt gttggtgtat tgggtaatgc tgctacaaat
     8161 gttacttttc cactaacaac aatatgtact gcaattgcat tattgttagg aattggagga
     8221 gcgtctaagt gttcattgga attaggtgct aaaaattcag aaaaagctac aaaagcagca
     8281 ggaaattcta tatgcttgat ggctgttttt ggaataagtt tatttatagt agtatcaata
     8341 tttttaacac cactattaaa attctttggc tctacatcag aaatactacc ttatgcacac
     8401 acatatacaa aaattacatc aattggatta ccatttctta taatgtccac tggaatgagt
     8461 aagttgatac ttgcagatgg aagaccaaaa gcttctatgt tttgtatgtt aataggagca
     8521 gttattaata caattttgaa tccattattc atttttgttt ttaatatggg aattgctggg
     8581 tctgctttag caactgttat tgggcaactt atatcttttg gaattagtgt tcattatata
     8641 aagaattttc aacatattaa attacataag aaaagtttta aattagatag tataatttat
     8701 aagcatatat ttgcactagg ttcaagttca tgttttaatc aattagcaat gactgtaaca
     8761 cagatagtta tgaataatac attggcatat tatggggcta ggtcagttta tggaagcgag
     8821 attccacttg cttgtgttgg tatcattatt aaagtaaata tgatttttat atctataatt
     8881 attggtatat cacaaggtat gcaaccaatt atagggtata attatggggc tgaaaagtat
     8941 aatcgtgtaa aagaagcata taagtttgca gtgggaagtg caactattat atctgttatt
     9001 gcattcttgt gtttccaatt cttcccaaga caaattacgt ctatatttgg taacggaaac
     9061 gagacttatt ttatatttgc agaacattat tttagaactt tcttgttctt aacatttata
     9121 aatggtgttc aaatagtatc atcaactttc tttacatcaa ttggtaaatc agtgttgggc
     9181 ctttttactt ctttgactcg tcaaatttta tttttaattc cattgatagt aattttacca
     9241 atgtttatgg gaattgatgg agtaatgttt gcaggaccaa tagcagatgc agcagcggca
     9301 atggtatgta tatactttgc agttcatgaa atgaaatcat tgactataaa gcaaaataat
     9361 ttgatgagta accataataa gattgcagag gtataagaca atatttaaga agtatagaaa
     9421 attaatttaa aaagtataat aagctaattt aagaaacata gaaaattaat ttaaagaaac
     9481 acaataatta atttttaagt aacattaaat taatgtaagt aaaataaggt tcaagtgaag
     9541 atttaaaggt gcattttctg aagccaagta tatgttgtag agtgttatgt ctctctatcc
     9601 ggtgaagttt aatagctcaa gaaaatgcac tatttatgat gattattttg ttttatttcc
     9661 aattttagaa ataactattc taattaaatc taaaattata ccataaataa gaaaagaaag
     9721 tatagatgct atgtaccaat aaatcgaaat tgaccctgta attgttctac atgggatttg
     9781 catgcctgaa tccataaaaa caaaaaaact atcaatagta gaaataatag acaaaatagg
     9841 atttaatcct attagaataa tactttttga gtcttgacca atttggtgca tataaatctc
     9901 aaataatgca attaaaaaaa accaaaatgt aaaagattta ataatttttt tcatcaaaat
     9961 acctcaccta atataaattc catttgcact atttgcttac tataactatt tatctccatt
    10021 cagtcacatc acttaattgt aatctaggtc ttcttgatgg ttcttcgtca ggataaccta
    10081 aagatattgc tgccactaat tgactatctg tatttaacca ttcacataat tcacgatatg
    10141 caaagtatac atcacaaatc caaagactac ctagacctaa ttccacagct gtaagcgaca
    10201 tattctgaat tgcagctcca attgattgca tgtttgccat ttcatagaat cgttcttcag
    10261 gggatagatt ttctaaaggt gatttaccta atatatttaa aataaaaatt gttactggag
    10321 cttgtttcat gatttcaacc gtgtaatttg caccagcgat atgttgacga cttcctggaa
    10381 gaagcccatt atcattaatt tcattttgaa tacctttgct catggcttta agcatagatt
    10441 ctttttcctt ttcagtgatg acaacaaaac gccatggttg cctgttttta gatgaaggcg
    10501 catttattcc agcttcaatt attttctcta tggtttcatg tgaaatgctt tgatttttat
    10561 attttctgat acttcgtctt tttgatatag aatcacttat cattttaagt aacctccgtt
    10621 tattttttgg aaaaatctat ttattttcta tttttaatta ttttagcatt aatcagaaaa
    10681 aaagtcattg attttagtat acaattatta ataattgtat taagaattgt atcaaaaagt
    10741 atgtgattat actaagggat tatattagac attatattta aaattgtatt aaaggttgta
    10801 tttaatatta tattaagact tatatatatt aggatttatg tatattaaga tttatattat
    10861 attgggcctt atattaaatt aagggctata atttgaaaat aagctataaa aactcaatat
    10921 tgttttgttc agagataaaa aaaggatact ttaggtgatt tttattaaaa attgagagtg
    10981 ataagattat atactttata aatgtaaata tgaggaataa aaatgataaa tataataatc
    11041 tatatattta cattaggagt tttctatact ataattattt aaaactaaat ataaatgttg
    11101 gaattattta ggatttgaaa ttgtctaaaa gtaaatataa gtgatgaaga ggtgatgtga
    11161 tgagattaga tatggatagt atgttatatg tactaattta tatgacatta ttggtatcta
    11221 tcattggaag tatattttgt ttttgtaaag acctcttaaa tcataaaatt aagtatgttt
    11281 taataagaga catatttgta aatagaacta catattctga agaaaggttg cctaaacagt
    11341 atatagtttt tcagaaatat gatatttgga ggtattgtag tttgttttaa tttgaaatga
    11401 atctattata aattctaaag tttaaaaata aaaaggagag aataaaatgg atttcatttc
    11461 aagtatattg caaggattat taagtatatt atttaatttt acaggtgatt ttggaatttc
    11521 tattgtactt ataactttaa tagttaaatt agtacttatg ccactatcat taaaacaaag
    11581 attttctgta aaaaaacagc aagaattagc tgaaaaaatg gaacatataa aagaaaagta
    11641 taaaaatgat actaaagaat tggaaaagca aatacaagta agttctgtag aaagtatgaa
    11701 gagtatgctg ggatgcctta tagttttggt tcaaatgcca gtagtatatg ctttatatca
    11761 tgtatgttta gatatgacaa aggaatttac aacaattata attccatgga ttgctaattt
    11821 aggtacattt gataatttgt ttatattacc ttgtatatat acgttaatta tgttagcttc
    11881 aaatttagtg aattatatac catatttaaa aataaattct caagtaaagt ttaacaaaca
    11941 aatggctgtg gtgactacta taacaagtct tatactgact gttagaactc cagtagccat
    12001 aggtctgtac tttataacaa gtgcaattta ttcattaatt gaagatattt gttttagaat
    12061 atattttaat cgaaaaaata aaatagtgac aaaataatat aattattatt agaaatattt
    12121 tagcctgcta acataggaaa agttagtagg ctatttttta tctatgatat caattccatt
    12181 aagaatagtc taaaaaatac tatcaaaaat actatcaaaa tactataaaa aaatagttga
    12241 aaaataaata aattaatata aactaaaata aagcctataa aaaagaggtg ataaatagaa
    12301 atggctaaaa tactagttgt agaagatgaa aaaaggatgc aaagtattat tgtagaatat
    12361 atgcaaaaag gaggatatac ttgtataact gctgatgatg gagtcgaggc acttacaata
    12421 ttaaagagca acaatattga tttaatgata ttggatatta tgatgccgta tttagatgga
    12481 ttttcagtgt gtagagtttc acgtgaaatg actaatatac ccattattat attgacagct
    12541 aaaggtgaag aggaggacaa attaaagggg tatgaatatg gtgcagatga ttatataaca
    12601 aaaccattta gcccaaaagt acttttagca aaagttaatg ccttgttacg tagatacact
    12661 actgatattc caaaaaattc attgagttta ggaaagattt ttattatggt ggcatcaagg
    12721 caagtgtatg tagaagataa attgattgat ttaacatata aagaatttga attattacgt
    12781 ttatttatgg aaaaccctaa tcaagttttt tccagagaga aacttttaaa ttgtatatgg
    12841 ggatatgatt ttgaaggaaa cacacgaact gtagatacac atataaaaac cttacgcaaa
    12901 aagcttggta gtgaaggaca ccatatagtt acattaattc gttcaggata taagtttgag
    12961 gttaaggaat gaatagttta agaaagattg tagaaaaact gaaaaatgta gatatttcaa
    13021 attggattaa acaaaatttt ggactatatt cagttagaaa aaaagtgttt ttactttcta
    13081 aattatcagg ggcgttaatt atattatttt atttgattgt tgaagaattt tctataaact
    13141 ctaaatttga attttggatt tggtttataa tactggttat atttattatg agtattgatt
    13201 ttttattggg aaaatttatt tcagaaccaa tcacatctat aaataaatct gcaaaaagta
    13261 tgtcccaact tgatttttct aatccatgta ctgtaaatac taatgatgaa tttggagaac
    13321 tttctaggag cttgaataca atgtctacaa atctacaaaa ggcattatca gacctagaaa
    13381 gtgcaaatat tcaacttgaa aaggatgtaa ataaggaaag aatgctattg gaacaaagaa
    13441 aggaattggt agatacaatt tcacatgaaa tgaagactcc acttggtatt atacgtgcgt
    13501 atacagaagg gcttatggat gaagttgatg aagaaaaaaa gaaaaattat atgaatgtta
    13561 tcatagaaga aactgataga atgaacaata tgattgtatc attattagac ttatctgcat
    13621 tggaagcagg agtttctaaa cttaacccag agcgttttga ttttatagag tttgttgaaa
    13681 ctgtggcagg tcgtctttta attgatatac cagatgccaa tttttatttt acatatgatt
    13741 tgccagagta tgaagttttt gtagtggcag ataaaatgag aatggaacaa gtagtagaaa
    13801 atctaattat taatgcaaag aagcatgttg tatatgatgg taatttagat ttatcaatta
    13861 catgtgaaaa tggcttgctt ttatttgaaa tttataatga tggtaggatt atagagcaag
    13921 aggagatttc aaagatttgg tctaagtttt atcgtagtgt tcaatctcag cgtacaggag
    13981 gttcaggtct agggcttgca attgtttcac aaatactctc aatgcaaggg ttagaatatg
    14041 gagtgaagaa tcgcgataaa ggagtagaat tttattttat gattccaatt gatgaataat
    14101 ttagtcttaa tttataaatt ataagtattt gatgtaaaat atttttcttt gtagacaata
    14161 gatatctaaa gagaataaaa taaaaaaagt ttaataaaaa aagattaatt agaaagtcta
    14221 ataaaaaaat ttaataaaaa gcttaatgag aagtcttgtt ttcctgatga aaacaagatt
    14281 ttttattaag ctttttatta tttcacttcg atttcacaag agagacactt tgatttcacc
    14341 aaatagaaat aaaataagtt aaataaatta aaaaaatata agaagtaatt aaatacgctt
    14401 acaagggggg gcacttatga aatggtatac atggttgact attatattgg tagtattggt
    14461 ttctattccg cttaaaatta aatttataaa atggtgggat agacgacaaa aagaaaaaaa
    14521 tgatgaagga ggctatgata gatgataaaa gtagaagagt tgaaattttc ttatggaaaa
    14581 gataaacaga tattgcatgg acttaatttt gaagtaaaag aaggagaaat atttggattt
    14641 cttggaccta atggttcagg aaaatccaca acacaaaaaa tcttaaatgg tgttttaaaa
    14701 ggttacaatg gtcacgtatc catttttggt aaggaagtta aagcatacac agaatcttta
    14761 tatcaaaaga ttggtgtttt atttgaattt ccttatttat ataccaattt aagtgcaatt
    14821 gataatttag aatatttttc gtctttttat cctaaaaatc aaaggagaga tatatatgaa
    14881 ctcttagatt tgttagagtt taagaaagag tttacaaata agcctgtatc atcctattca
    14941 aaaggtatga agcaacgtat tagcatggca agagcattgg taagtaatcc aaagttgttg
    15001 tttttagatg agcctactag tggacttgac ccatctggag ctgtactatt tcgtaagatt
    15061 attgaagaag aacgaaaaaa aggaacaact atctttttga ctactcataa tatgctagat
    15121 gcagatttaa tgtgtaacaa tgtggctttt attgcagatg gcaagattat ggcaatagat
    15181 aaacctaaaa atttaaaaat gaaaaatagc aataataaag ttgaagtgga atttttatat
    15241 aatggaaatc gagatattga aattgtagat atacaagagt tagaatctgg aataa
//
LOCUS       DAEORZ010000100        15219 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEORZ010000100 DAEORZ010000000
VERSION     DAEORZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.100"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            79..936
                     /locus_tag="KRH39_002676"
     CDS             79..936
                     /locus_tag="KRH39_002676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG8512568.1"
                     /translation="MYKYMNDVEIIINNMIDVFGINVKKSATFYKSRINNFFEIYMGT
                     DGNEYKPLDALTYYDVEIYLGKLRCSDAEKLNVYNALKRFFEYTYLEGKTKEIISQVK
                     RPTCIKKPNEVLNEEEYLKLKSFIVSRNENLNVRLILGLFLFTGLSRKYIANLRNNDF
                     KFDEGVYKLIIWKGDEEIQLPLKSELQLIINEYCNNLSDEEKFDKLVKISENAISDYI
                     SKLIDSIIGRRCTPTILSNTFISKALSSGNYIYEVSNLTLETVHTIAKHVKNDKDLIN
                     RQTSILNSF"
     gene            1317..1511
                     /locus_tag="KRH39_002677"
                     /pseudo
     CDS             1317..1511
                     /locus_tag="KRH39_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
     gene            2140..2934
                     /locus_tag="KRH39_002678"
     CDS             2140..2934
                     /locus_tag="KRH39_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG8512569.1"
                     /translation="MESVMENLNNLKVELKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            3029..4369
                     /locus_tag="KRH39_002679"
     CDS             3029..4369
                     /locus_tag="KRH39_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG8512570.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            4506..5738
                     /locus_tag="KRH39_002680"
     CDS             4506..5738
                     /locus_tag="KRH39_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG8512571.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            5778..6920
                     /locus_tag="KRH39_002681"
     CDS             5778..6920
                     /locus_tag="KRH39_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG8512572.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            6931..7725
                     /locus_tag="KRH39_002682"
     CDS             6931..7725
                     /locus_tag="KRH39_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG8512573.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            7736..8758
                     /locus_tag="KRH39_002683"
     CDS             7736..8758
                     /locus_tag="KRH39_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG8512574.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            8857..10233
                     /locus_tag="KRH39_002684"
     CDS             8857..10233
                     /locus_tag="KRH39_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG8512575.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(10565..10909)
                     /locus_tag="KRH39_002685"
     CDS             complement(10565..10909)
                     /locus_tag="KRH39_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512576.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            11397..12407
                     /locus_tag="KRH39_002686"
     CDS             11397..12407
                     /locus_tag="KRH39_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG8512577.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     IF"
     gene            12543..13031
                     /locus_tag="KRH39_002687"
     CDS             12543..13031
                     /locus_tag="KRH39_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8512578.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            13207..15135
                     /locus_tag="KRH39_002688"
     CDS             13207..15135
                     /locus_tag="KRH39_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8512579.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
BASE COUNT         5815 a         1690 c         2885 g         4829 t
ORIGIN      
        1 tgaaataaaa aggtttttat cataaaatgt agaaatgtat attaaataga ttaaatgttc
       61 tagtaaagga gtattgatat gtataaatat atgaatgatg ttgaaattat aataaataat
      121 atgatagatg ttttcggaat taatgtcaag aaaagtgcaa ccttttataa aagtagaatt
      181 aataattttt ttgaaattta tatgggaaca gatggtaatg aatataagcc attagatgct
      241 ttgacatact acgatgttga aatatattta ggaaaattga gatgtagtga tgcagaaaaa
      301 ttaaatgtat ataacgcact aaagagattt tttgaatata cttaccttga aggtaaaacg
      361 aaggagatta taagccaagt aaaaagacct acttgtatta aaaaaccaaa tgaagttctg
      421 aatgaagaag aatatctaaa gttaaaaagc tttatagttt ctagaaatga aaatttaaat
      481 gtacgtttaa ttttaggatt atttcttttt acaggtttga gtagaaaata tattgcaaac
      541 ctaaggaaca atgactttaa atttgacgaa ggtgtatata aattaattat ttggaaaggg
      601 gatgaagaaa tccaacttcc tttaaaatca gaactccaat taattataaa cgaatattgt
      661 aacaacttat cagatgaaga aaaatttgat aagcttgtaa agataagtga aaacgctata
      721 agtgattata tatcaaaatt aatagatagt ataataggga gacgttgcac tccaactata
      781 ctatcaaata cttttatatc taaggcactt tcaagtggaa attatatata tgaagtgagt
      841 aatttaacat tggaaacagt tcatacaata gcgaagcacg taaaaaatga taaagacttg
      901 attaatagac aaacatcaat attaaatagt ttttaaataa aatgatggtt tgttttaagt
      961 aataatcaga ttacttttat aatctaataa agataagcga gtgtcaccaa actaaaaaat
     1021 ggagcaattc catttttata caacaaagat aaattagtta aaaagctgtt acataacaat
     1081 tataaattgt tatgtaacag cttttttaca tttaaataac tttgaaagag catgtttata
     1141 aagttaagaa tcattatagc aggttttgag cagggcttga ttgcttataa ataattgatg
     1201 ggtatataac ttgttgctaa atactgttta tacgagcggt tactgtatca ataatcatcc
     1261 taatactacg ttctgccgtt tcttccattt taaaatctat aactatcaaa tgaggaatac
     1321 ttcttgacat tagtcatcct attataggaa atatgaaagt caatggaaat ccagttaagt
     1381 tgatgacaac aaaagctaaa attgatatgc cagcaccatc tctaggggaa agtaacgagg
     1441 agatttataa tggttggctt ggttttgata gtgataaaat aaaagattta aaagaaaatg
     1501 gagttatttg attaaaaaat agctaaacac acttgtgact ttagtcatgg gaggttcagt
     1561 tagaataata tcaaaaaata ttaaatcaat actgtaaatc aaatatagaa ttgtcagaag
     1621 catttaagat gggaaaagct acttagacat tgagtctttg tggcttttcc tattttcatt
     1681 aagaatttta tattatcaga atagatatgt actaaactaa agtattaaca atttaaatta
     1741 atagaaaata aactacctaa ttatgttgaa taaagactac tttagtgaaa ataaacttat
     1801 agagatgcta tttaaattat tttaatggaa atatcaaaaa tgagactgca ttctcaaaaa
     1861 tgagactgcg ttttttaagt attttctcga aaatgagaca aaaatattgt tttatgggtt
     1921 ttattgaaga aaaattcttc tgtgaattac aatttaatca attttaaagt tgaaaattat
     1981 taaaaattta gtgaaattta tgtatatttc gtttatttta ttaaaaataa caatttttta
     2041 ctaaaaataa agaacttcaa tggtgattgg catagtactt gctattttat attaacaaga
     2101 aatgtagaaa aaataacaat gtataatggg gggaatatga tggaatctgt aatggaaaat
     2161 ttgaacaatc ttaaggtgga gcttaaggac aaagtctgtg ttattactat aaatagacct
     2221 aaagctttaa atgctcttaa tagtgacact cttagggaac tatcacaagt tattgatgta
     2281 gtatcagaaa atgaggcaat attaggagtt attattactg gagaaggtaa agtttttgta
     2341 gctggtgctg atatacgcca gatgcaaaac tataaaagtg aggaaggcag aaaatatgca
     2401 ggttatgctc agggaatttt tgataaaatt gaagctcttg aaaaaacagt aatagcagca
     2461 gttaatggat atgcattagg tggtggatgt gaacttgcta tgagttgtga tattagaatt
     2521 gcatcagaaa aggctatatt tggacaacct gaagttaatt taggagttat tccatgtttt
     2581 ggtggaactc aaagattatc aagacttgtt ggaactggaa tagctaaaga gttaattttt
     2641 acagggagac aagtaaatgc agaagaagct aaatctattg ggcttattaa taaagtggtt
     2701 ccaagtgatt tacttttaga agagtctatg aagatgatga accaaatagt tgaaaaagcg
     2761 ccaatagcaa taagatatgc aaaagttgtt attaacaaag ggatagatat ggatttaaag
     2821 aatgctcttg aactagagaa agatattgca ggtttaactt ttgctacaag agataagcaa
     2881 gaaggaatga atgcatttat agaaaagaga aagccagttt ttgaaaataa gtagctatta
     2941 taactaaaat tggaacaatg tagaatatat tcaaaatcta aatttcgtga aaaaatacat
     3001 atagtttaat tttaaaagga gttaatttat gaatacggga gtaattataa gtttagtagg
     3061 gattatcata gctctatctc ttctagttgt aatggttatg aagggaataa atatttttat
     3121 tatagcaatt gtatgttcat cattagttgc tataacagga agcctaaatg tgtatgatgc
     3181 acttaaagtg gattatatga cagggtttgt tggatttctt caggcaaatt tctttatttt
     3241 cctaacaggt acattaatgg gaaaaataat ggagattact ggtggagcaa aatctattgc
     3301 taagatgatt gtaagatgga ttgggaagga taaagcacta ctttctattc caatagcttg
     3361 tggaatatta gcctatggtg gagtaagtgt tttcgttgta agttttgcag tgtttccgat
     3421 agctttagaa atttttaaag aagctgattt gccacgaaga tttataccag cagctttgac
     3481 ttttggatgc tcaacttttg ctatggttgc accaggggca ccacaaattc agaatattgt
     3541 tccagcatca acattaggga cagatattat ggctggagca gtaaatggtt ttatatcatg
     3601 tgcagtaatg tttatagttg gaagtaccat tttgtacaaa atggtttcaa aagaaaaagc
     3661 caatggtggt cattttatag cacatgaatc agatatattt gatgaagcag aagcaactaa
     3721 ttctaagaga gaaaatggtc ccaatggtgt aattgcctta attccattga tagtgtcaat
     3781 tttaataatt aatgtaaaag ttaatggaaa agcgatagta ccaattgagg tgggagtatt
     3841 tattggagct ttattagtat atttattgtt aaataaatat caagataata ctaaaatagt
     3901 tggacatgtt ggtgatgcat gtaaaactac agtagtagca atatgtaata cttgtgctgt
     3961 agttgcattt ggttcagtag taaaatcagc agttggtttt gacttcatag ttaatgccat
     4021 gactaatatt cctggtcctc ctattgcagg ggctgcagtt ggaactacag ttattgcagg
     4081 tatttgtggg tcagcttcag gtggactagg aattgcagct ccacttcttg gaccagtatt
     4141 tttagccaag ggtgtttccg ctgcagcatt aactaggact atggcaattt catcagcagc
     4201 attagattca cttccacaca atggatatat tgttactgta acaaatggtc tttgtaaaga
     4261 aacacataag gatgcctata tgccaatatt ctgggtaact gttttaaccc catttattgg
     4321 aaccatagta gcagtcctac tgtttacaat gttcccggga ttgccataag agaaaagata
     4381 aaaggtataa attatatttt gatattataa aatcaatttt caaaatatat acaataaatt
     4441 tcatgtttta atactgtaaa tatgtattta aaataacaaa ttgaccatat aaggaggaat
     4501 tgaaagtgtt aaataaacca ttagagggag ttaaggttat tgacctaacg tattttgtag
     4561 caggtccagg gacttctaaa atattagctg actggggtgc tgacgttatt aaggtagagc
     4621 caagctttgg agacccagga agaaagacag gggcaacaat gacaatgcca atagatgatt
     4681 ataataatcc tttttacagc acatataatt caaataaaag aggtttgtct ataaatctta
     4741 agagtgaaac tggaattgaa attatggata agcttttaag tgaagcaaac gtatttgttt
     4801 caagttatag aacaggagca ttaaaaagac taggtcttga ttatgaatca ttaagtaaaa
     4861 agcatccaca ccttatctgg ggtcagataa atggttttgg tgattttgga cctgctaagg
     4921 ataatgcagg atttgataca gttgcctttt gggcaagaag tggggctatg cttgatatag
     4981 cagaaaaaga tacaagtcca ataaatccac ctatagcctt tggagatgca acaacttctt
     5041 gtagtttatc tggaggaata tgtgcagctt tgtaccagca agctaagact ggaaaaggac
     5101 aaaaagtaat ggcatcttta tttgggcaag caatatggaa tgcatcatct ttattagcat
     5161 ctacacagtt tagtgatgag tatccaaaaa ctagaaaaaa tgctatttca ccagtaataa
     5221 attcttatcg ttgtaaggat ggaaagtgga tatttttaag tattttagag catgaaagat
     5281 attttgaggc actttgtaga ttatttggaa gagaagactt aataaaagaa gagaaatttg
     5341 catcttcagt agaaagtaag aaaaatgcta cagaacttat taatattttg gatggtgaat
     5401 ttgcaaagtt tactcaaaat gagatggttg aaaaacttac tgctgctgat atagctcatg
     5461 aaaaaattca acatgttaat gatgtagtga ctgatgaaca ggctattgct aataactata
     5521 tatatgaaca tacaagtaaa aatggaaata agacaatgct tgcatctact ccagttaaat
     5581 ttggaaatat tgaagttaac atgacttgtg atgctccact tataggagaa cattcagatg
     5641 agatattgag agaacttgga tacagtgatt ctgatataga agctctaatt gaatctggaa
     5701 ttgtaacaat gaataaagaa gcatgtgaaa tatgttaatg aacatgtgaa gtatgttaat
     5761 aaataggagg gaattttatg gattttagat taacagaagc acaattaatg cttcaaagag
     5821 tagcaaaaga atttgcagag aatgaaatag caccaatagc tgctgaaaca gataaaacag
     5881 gtatttttcc aagagagctt tttagcaaaa tggctaaaat aggatttaat ggcattggaa
     5941 cacctgtaga gtatagtggt tctggtggag cagatataga aaaggtaatt gttgttactg
     6001 agattgccaa aaaatgtgca gcatctgcag caatattatc aatacacaca atatatgctc
     6061 aagctatttt gaaatttggt actgaggaac aaaagaaaaa ataccttcca atgatggctg
     6121 aaggtgggtg tgtaggtgct tttgccttaa ccgaacctaa tgctggttca gatgctgcaa
     6181 gagctgcaac aactgcaatt attgatgaag aaactgatga atatgtttta aatggaacta
     6241 agtgttttat ttctggtgga ggtcaagcag aatcattaat aatatttgca ctaacagacc
     6301 catcaaaggg aataaaggga atgtctgcta tcattgtcga caaaggtact ccaggttttt
     6361 caataggaaa aattgaagaa aaaatgggta ttcatggttc agaaactgca gagttaatat
     6421 ttgataactg tagagttcca aagtctaact tattaggaaa agaaggcaaa ggatttaata
     6481 tagctatgac ttgtttagat ggagcaagaa ttggagttgg agctcaagct gtaggaattg
     6541 ctgaaggggc tttagaagaa agtatcaaat attctaaaga aagagtccag tttggcaagc
     6601 caatttcagc tttgcaaggt attcaatggt atatagcaga tatggcgaca atggtggaat
     6661 cagcaaaatt attagtatat tatgctgcag atttgaaagc tagaggagaa aaacacacta
     6721 aagaagctgc tatggcaaaa tacaatgcgt cacgtactgc aagagaggtt acaaatttag
     6781 cccttcaaat ccatggtgga tatggatata tgaaggatta tccattagag agaatgtaca
     6841 gagatgccaa aataacagaa atttacgaag gtacttcaga aatacacaaa gttgttattt
     6901 caagagcagt actaggatag gaggaaaaga atgaatatag ttgtttgctt aaagcaagtt
     6961 ccagatacta atgaagttaa aataaataaa gagacaggta ctctaataag agatggtgta
     7021 ccaagtataa tcaatccaga tgatagaaat gctttagaag aagctctaaa aatgaaagat
     7081 gaactaggag cagtgattaa ggttataagc atgggacctc ctcaagctaa aagtgcttta
     7141 aaagaagctt tggcaatggg agctgatgaa gcttatttaa tctcagatag agcttttggt
     7201 ggttcagata cttgggctac atctacaatt attgctgctg ctatagagaa ggttggaaag
     7261 tatgatgtaa tattctgcgg aagacaagct atagatggag atacagctca ggttggacca
     7321 gaggttgcag aatttttagg gataccacaa gttacttatg cgaaagaagt aaaggttcaa
     7381 gatgacaaat tactagtaac tcgttataca gagacagggg attatttaat agaagcaaag
     7441 atgccagttt tattaactgc tataaaagag ttaaacaatc caagatatcc aagtgtcaaa
     7501 ggaatcttag aagcttataa taatggagat gcaaagatta ctgtattaac acttgcagat
     7561 ttagatgttg atacaactca aattggatta aagggttcac ctacaaacgt ttataagtct
     7621 tttgtaccag ttaaagacaa gcataatgaa ataatagaag gtataaataa aaaagaaaaa
     7681 gctgagaaac taatagaaat actattcgat ttgaaactag tataggagtg ttaagatgat
     7741 gagagcaaaa gttaatcaag gtataaattt aaatgattat aatggtgttt gggtaatagg
     7801 agagcaaagg gaaggtaaaa ttaatcctgt tacgatagaa cttattggtg aaggaagaaa
     7861 gcttgctgac caattaggaa aagagcttgc agttgtgata gctggatatg aagtagagaa
     7921 agaagtgaag gaattacttc actatagtgt tgataaagta tattacatca atgaccctct
     7981 tcttaaagat ttcactactg atggatatgc aatttcaatt gcaaatttaa tcgaaagaaa
     8041 aaagccagag gttgtattgg ttggtgctac ttctatagga agagatatag cgcctagaat
     8101 tgcaggtaag gttggaacag gtctaactgc tgactgtaca aaacttgaaa tagattcaac
     8161 agataataag ttattacaaa caagaccagc atttggtgga aatttaatgg ccactattgt
     8221 ttgtccaaaa aatagaccac agatgtcaac agttcgtcca ggagttatgg caaaagctgt
     8281 aagaaacgaa tcagagactg gaattttaga agttgttact ccagaactta ctgaaaaaat
     8341 gattagaact agattagtag agatacttcc acaagaaaag aaatctgtaa atctaactga
     8401 tgctagaata atagtatcag gaggaagagg attaaaaaga gcagaaggat ttgagcttat
     8461 aaaagaattg gctgataaat tgggggcaga gattggtgct tcaagagcag cagtagattc
     8521 agggtggata gaacattctc atcaagtggg acaaacagga actactgtta gaccagaact
     8581 atacattgca tgtggaatat ctggagcaat acaacattta gcaggtatga gtgattctaa
     8641 atatatagtt gctataaata aagatgcaaa agcccctatt tttagtatat gtgactatgg
     8701 aatagtagga gatttatatg aaattatacc tgaaatgata gaatcattaa ataggtaact
     8761 tttagaaaac attgaatatg tagaatgata agtcttataa tattgtattg taagacttat
     8821 tatttttgct gaaggttata gtggggagat gaaacaatga aagagcagtg gtataaggat
     8881 atatttgcaa gagtattatc tatgactgat gatggtttta ttgttgtaaa tacaagtgga
     8941 gtaataatag atattaatga taaatattgt gactttttag gaaaagaaag aaaagatata
     9001 ataggacaaa atatacaaag tattatacca aatacaaaaa tgctagatgt tatgaaaaat
     9061 aagtattgtg aagaaggagc tatacatcat tattcaggtg gaaatacaaa agaaaaaagt
     9121 gtaatagtaa gtaggtctta tgtggaaaat gacaatggag aagtagttgc aggagtagct
     9181 caagttaaat ttagattgca atcatttgat gtagctaaaa agctaatgtc agaatatatg
     9241 gagctccaat attataaaga gcagtttaag gataactgtg gttttgacaa gctcataggt
     9301 gaaaatagag acttcataga acttaagaaa acaggtgtga aagcttctaa aacaaacttt
     9361 ccagtacttt taacgggtga aacaggtaca ggaaaagaag tctttgctag agcaattcac
     9421 aataatagca gcaggtcaga taaacctatg gttagtataa actgcgctgc aataccagaa
     9481 gaattattag agtcagagct atttggttat gacgaaggag cttttacagg agcaaaaaag
     9541 ggtggtaaaa aaggtaagtt tttagttgcg aataatggta caatcttttt agacgagata
     9601 ggtgacatgc ctttgaccat gcaagcaaaa cttttaagag ttcttcagga aagtgagata
     9661 gaaccagtgg gaggtcttaa aactataaag atagatgtaa gagtaatctc tgccacaaga
     9721 aagaatttaa gtaaaatggt tgaagaggga ttatttagag aagacctata ctatagatta
     9781 aatgtaataa acattcatat gatggaattg aaggacaggc aagatgatat tttattgcta
     9841 gcaaattata tattaaacaa attaaatgtt gaatacaagg aattgaaggt tttaagtgat
     9901 aaggttaaaa attgttttat taactatact tggccgggaa atataagaga actacaaaat
     9961 gtaataaaaa gtgcctatgc tgtaagtgat gatatggtca ttatgatgtg tgaccttcca
    10021 tctaagatgg ataatatcag cagagtagct caatgtaatg tggatagcaa ttgttcaatt
    10081 catgaaatgg ttgagaatta tgaaaagagc ttaattattg atgtgttaag gaagtataat
    10141 tggaaatgtt ctaaggcagc agaggttatg ggaatacaca agagtctttt atataagaaa
    10201 ataaaaaagt atgaaattga attaaataat taagataaat tgaattaagg caatgctaaa
    10261 aaaacgccct tattcaaagc gaactgtata aactgtttta tgcagttaaa acgtgtaaaa
    10321 gtagatgtga agtactaatg tcaatcatta gtactttttt attatattta taattactat
    10381 ctatatttca gtatcttttg atatctaaga ttattatgtt taatttagga tataacaaaa
    10441 ccctcttagt tatggacagt tttttatatt ttaactgtct actaagaggg gatagtttag
    10501 tttaaattta gagtaaattt tatctagcat ttataaaaaa tattttgaat agcttttttg
    10561 ttatttaatc ataattttct acttctttat caagttcttt gccacaagaa tcacacttat
    10621 atatagcatt gtatccgtaa tcatattcat caactatttt cttttgtcta aatacaccac
    10681 cacactcaca taggaaacca atttttataa gtgtaacgat aaattgtgat tttataaatt
    10741 ttatatcatt acatttatcg ttagtgtaaa atgtataaat ataagagtta tcgcactcat
    10801 ctcttaattc aaatctttca atatatgtgt tattttttaa ttcctcaaat tcttctttag
    10861 ttaaatatga tttttgttgc aattcatgta aacgcttaat ttggtccatt atttatatcc
    10921 tcccaataat tttagtattt taagcttaga taaggaatct aagttttatt aaatttatcc
    10981 caaggaatta tttaaatcat atttagtttt attaattaca acatatataa attacacgaa
    11041 aataaaacta attgtaaaaa taatatcctg tgaaaaccta aactactaac cccttactaa
    11101 cccgaaaact aaaaaaagaa aatatatcca aatataagtg atatataaat aaagatgttt
    11161 catataaatt taactttaaa tattctgaca ataatatata taaatatatt atagttaaaa
    11221 tataccacat attttgggta ttgtatactg gaattagagt aattaattta ttttttaaca
    11281 tacaaatgat tttatgaaaa aaaacaagac tcaaaaattt ttatattctt tgatagaata
    11341 aaattgagag taaatttatt gaattttata aaatttataa ggggtgagtc taagatatgc
    11401 atgaagataa aaatgatata tttgaatcaa tagatactga ggattttgat aataaaacag
    11461 aagaagaatg tttagagcta gtaagaaaaa atggattgaa tttaatatac ataaaaaatc
    11521 aaacagaaga gatatgtcta gaggctgtta gacaaaattg taatgcaata aaatgtgttc
    11581 agaatcagac agaaaaaata tgtatagaag cagttaaaca agattggaga atgttagaat
    11641 ttgtagaaga gccaacagaa gaaatttgca tgttggctat taaccaagat ggaacagcat
    11701 taaaatatat agaaaatcag acagaggagt tatgcttaag agccgttgaa aaaaatggtg
    11761 cggctttgga gtatgtaaaa gaacaaacag aagaaatatg tatagaagca gttagaaaca
    11821 gcgaatttgg attggcaaga gtgaaaaatc aaacagaaaa aatatgtatg gaagcagtta
    11881 agcattgtag ctataatcta aaatgggtag aaaatcaaac agaagaaata tgtatggaag
    11941 cagtgagaca aaatgggctt gatttgaaat ttgtaaaaaa tcaaacagaa acaatctgtt
    12001 taagagcagt gagacaaaat ggaatggcat tagaatttgt aaaggaacag actgttggta
    12061 tatgcttaaa ggcagtaaga caatatggaa tggcattaaa atttgtaaaa gaacaaacag
    12121 aagaaatttg tacagaagct ataaaacaag ataaaagagc acttagtttt gtaaaaggag
    12181 ataaggaaaa atacaaggct ctatatgata ataatgaacc atttgcaaag agatatgtta
    12241 gaaatgtaat agaaaaagaa aacagagcat tgattaaaaa aggagaagaa aatgcaaaaa
    12301 tcaaaattgc aggaaaactt tatgatagag gcatgtcttt tgaagatata tcagatatag
    12361 ttgagattga aataagcaag ttaaaagtga gtttaggtat tttttaaagt aataaatgta
    12421 gacgtttttt atgggaaata gttaataaaa aaataatcga ctagctcttt gtaggacagc
    12481 aaaattagtg atataatttg atgtataata aattattttt attagataag ggggaattaa
    12541 agttgaagat tacagtatta taccaaacaa gaagtggaaa cacagaaagg gttgctaagc
    12601 tgatagaaga tggagctaaa aaggtggatg gagtagatgt aaagcttatg agacttgata
    12661 atattgattt agattattta aatgaaagta aagcagtaat atttggaaca cctacttatt
    12721 tagcaaatac aacttgggaa gtaaaaaaat ggtttgatga agattcaaaa aaagttaatt
    12781 tagctggaaa attaggagct gtttttgcta catgtgatta tatatgtgga ggaccagatg
    12841 ttgcgatact tactatagta ggacatttga tggttaaagg gatgcttgtg tattctggag
    12901 gtggttcttt aggaaagcca tttatacatt taggacatgt acattcaatt gaaggtccag
    12961 aactgcaaga tgagaaggct ataatatttg gtgaaagaat tgctactaag gcaaaagaac
    13021 tatttgctta atttctatat gcataataat gattagagta aatttaggat tttaatggag
    13081 atttgaagta aaagttataa aataaaaaat tttccataaa attactcaag atgaattata
    13141 atataattat aaataaatta tattattaaa atatttaaaa tcaaacgtca atagcgaggg
    13201 aagtttatgg tatggcatat agaatatgag attttttctg caattatagt tattttttta
    13261 atggtatact tttttagaag taaatttata cctactttac aaaacaaaat atattgtgca
    13321 cttttaatat tctcattttt atttataatt tcaaatattt taggttcatt ttgtctaaat
    13381 aatatagaca aaattccaat ttttattact tttttattga atcagatata cttattgtta
    13441 ttgccaatac cagcagcact tgtatcattt tatgttatgg ctattattta tcaagatata
    13501 aggtatatga aaaaaagatt attattttta tctataccac ttataatttg tataatctta
    13561 tctattacaa atccttttac gcatattcta tttggattgt caaaagaaac aggttatata
    13621 cgaggttgtg gatatgttgc aacttttgca tcttttttct tttatgcaat atattcagca
    13681 tttttatctt ttaaatatag aaaagcaatg catgaatcaa aaatatgggc tattagaggt
    13741 tttcttatag tttcaattgt ggcaattatc ttacaggcaa tttttttaca gtatcttctt
    13801 acaggaactg catgtgcatg tagtttactt ttgacatatc tttctatgca gaatcgaggt
    13861 cttattattg atgaccttac tggtgtactc aatagacaat cttttattca agagctagat
    13921 ttgaatatca atactgaaca atcaggattt attattacaa ttgcgcttga tgattttaag
    13981 tttatgaatg aaacttttgg aacaaaaaat ggagatattg cactaaaaga agttggaaaa
    14041 tatttgattc agatacatga tgtaaaccat gtatatagaa tagggggaga tatattttct
    14101 attgttgtca atagtcaatt aggtatagaa ccagatgata ttatcagaaa gatagaagaa
    14161 agatttaaaa agccttggct agtagaagag ataagtttta atttagcaac tagtattgct
    14221 gttgtttatt acccagaaaa tgcagaaacg acagaagatg taattgttgc tatagatttc
    14281 tctatacatg aagcaaaacg ttctgagtca aggcgagttg tttatgcaga tgcgtctata
    14341 agtgaaaaga taaagaagaa acatgttatt aaaaattgtt taaaagaagc attaaaaaat
    14401 gatgggtttg aagtgtacta ccaacctata ttttctaata gcaggggcag gtttacttca
    14461 gcagaggcat tacttaggct agaacacaaa gagttaggtt ttatacctcc tgatgaattt
    14521 attccaattg cagaacaaac aggattgatt aactcaattg gattagtagt ctttgagaag
    14581 gtatgtagat ttatagctag tgatgagttt gaaaatttaa aattggataa tatagctgtg
    14641 aatctttctg tagtacaatg tatgcaaaaa aacttagctg atgatttact ttatcttatg
    14701 aataaatata atgtatcacc aactaaattt aaacttgaaa ttactgagac tgtggctatg
    14761 ggctctttta atataattaa agaaacaatg gaaagattaa ttgatttagg tgttaagttt
    14821 gcacttgatg attttggaat tgcttattct ggagttacta atatgctttc tttaccattt
    14881 tcagtaatta agttagataa gagtttgatt tggtctatga atgaagatag taggcataaa
    14941 cttacagttg agacgattat agcacttata aataaattga atatgaaggc tgtagcagaa
    15001 ggtgttgaaa ccattaagtg tgctgaagat ttaatagcta tggagtgtga atatttgcaa
    15061 gggtattatt tttctaaacc agttccagaa gatgttttta aaaatttgtt gaaagaaaat
    15121 acttttacaa gttaacaccc tttatttttt taattacaac tttgttcatt gacactgcca
    15181 gcgatatatt ataaaattaa cacaataata aatattaaa
//
LOCUS       DAEORZ010000101        14985 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEORZ010000101 DAEORZ010000000
VERSION     DAEORZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.101"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..2295
                     /locus_tag="KRH39_002689"
     CDS             <1..2295
                     /locus_tag="KRH39_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG8512580.1"
                     /translation="KRIKKEISKQLYKNKTIELEYRIITKNGNTVWVLDKGELITEPD
                     GEEYFCCVTVDITRTKQLEQEMEQKNLELEMISENILGGIIITEFNDDFTIKYTNAGY
                     LSMIGYTREQLEKELKYKACNLIFYEDRKKALESIQHQLNTEGIAVYQHRIRRRDGNI
                     IWVSIKGRLIIDDDGIEKGIWILNDITDLKNAEQKLKINEERFRIALKQTSNTIFEYD
                     IKTKSLLNTSKELDKYNIYKITKNVPYSLVESGVIHKDFSDDFINMYENVINGAKKAS
                     CVVKMKLPDENYIWNKVTLTTIFDEDGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSA
                     ILSEAMITYEINVSKDTFIKGNENWQKPFGIKPDNNYSQMVKIIANSIVHKDNKETFL
                     NTFLCKKLLDAYKNNVTEIKLEYRRMNQENKMIWVICTMHLIKDSITNDIKALAYVKD
                     IDKQKNEEIRLKLKAERDTLTGLYNKGTTEKLINDFLNSEEASLGMHAFLIVDIDNFK
                     SVNDNLGHVFGDKVLFEVSKHLKPIFRKDDIVGRIGGDEFIVFLKNIHSFKNTKKKAE
                     KICDIFRNSYTENKKTYKISGTVGISFVPEHGTVFEELYKKADIALYYAKSKGKDRYA
                     LYNDCIHQNELENNISDNNIKLADKYEESKPVLKEILDSIDNYIYIVNPNTYELLFVN
                     NKVSRLDLDIKLGETCYKSLLKFDRPCNNCPLNGLEENKHSKFKIETNTFGKNISIAS
                     WINCIDGEYNCLIECIDSSLYKNK"
     gene            2313..3059
                     /locus_tag="KRH39_002690"
     CDS             2313..3059
                     /locus_tag="KRH39_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBG8512581.1"
                     /translation="MLNSITLILGIIFTTYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVATESLIIFYPKNSLESKSDYLLILGASVKK
                     TTLSTTLKGRLDTALKYLKIDDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHNERKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIVFDR"
     gene            4363..4710
                     /locus_tag="KRH39_002691"
     CDS             4363..4710
                     /locus_tag="KRH39_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG8512582.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(4860..5360)
                     /locus_tag="KRH39_002692"
                     /pseudo
     CDS             complement(4860..5360)
                     /locus_tag="KRH39_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(5387..6892)
                     /locus_tag="KRH39_002693"
     CDS             complement(5387..6892)
                     /locus_tag="KRH39_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG8512583.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLINLNKELDTLEKQKE
                     RLHDFLEQGIYDSNTFGERLKILLSRIENAREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
     gene            complement(7091..7945)
                     /locus_tag="KRH39_002694"
     CDS             complement(7091..7945)
                     /locus_tag="KRH39_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8512584.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQPQGVLGISICNNSEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            8330..8611
                     /locus_tag="KRH39_002695"
     CDS             8330..8611
                     /locus_tag="KRH39_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512585.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            8758..9590
                     /locus_tag="KRH39_002696"
                     /pseudo
     CDS             8758..9590
                     /locus_tag="KRH39_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
     gene            9642..10163
                     /locus_tag="KRH39_002697"
     CDS             9642..10163
                     /locus_tag="KRH39_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG8512586.1"
                     /translation="MIPLLILGLLKQSPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSHHLVN
                     IEWFEKLLNDIRD"
     gene            10514..11047
                     /locus_tag="KRH39_002698"
     CDS             10514..11047
                     /locus_tag="KRH39_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBG8512587.1"
                     /translation="MTEVIIVNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVD
                     NKMTAQDLTQDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDK
                     EISNQELSDIENKMFIKEALTKLPELEFEVIILRYYHDLKFKDIAKIMESSVSVTKYR
                     MSRALKKLELYLEGGKL"
     gene            11044..11805
                     /locus_tag="KRH39_002699"
     CDS             11044..11805
                     /locus_tag="KRH39_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512588.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFLILCTICIFLFSTSADSNSFYNSLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGMSDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAVTLIYEIRKTWRHLCSFENIII"
     gene            11843..12709
                     /locus_tag="KRH39_002700"
     CDS             11843..12709
                     /locus_tag="KRH39_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512589.1"
                     /translation="MELSVECLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVRFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSIEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            12730..13971
                     /locus_tag="KRH39_002701"
     CDS             12730..13971
                     /locus_tag="KRH39_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter permease"
                     /protein_id="HBG8512590.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNIVTNESGFSDF
                     KPDIYWKFFYPNKTFYNFIIESYSKPNDFNPEVIKSMPLENEINFYKFRNEKVNTILN
                     MEYPEGSYTQKEKDFWLSKNKDIKEPYLNGYYQGWESFLLARDLLIFTIMAVCISIAS
                     VFSGEYQSGADSVVLTSKYGKTKVITAKIVSSFLFGTIIFTINTIVSIGILLLSFGID
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLIIYMILILLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFLKYSETNKLYNQILNVLPSNAISFSFTNYVDYKFGNLIIALPTMIIFTC
                     LVLTVVFLPFIKNNFKKHQVN"
     gene            complement(14195..14767)
                     /locus_tag="KRH39_002702"
     CDS             complement(14195..14767)
                     /locus_tag="KRH39_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8512591.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQKSV"
BASE COUNT         5775 a         1779 c         2073 g         5358 t
ORIGIN      
        1 aaaagaataa aaaaagaaat ttctaaacaa ctttacaaaa ataaaactat tgaattagaa
       61 tataggatta taacaaaaaa tggtaataca gtatgggttt tagataaggg agagcttata
      121 acagaacctg atggagaaga gtatttttgt tgtgtaactg tagatattac tagaacaaaa
      181 caattagaac aagagatgga acaaaaaaat ctagaacttg aaatgatatc agaaaatatt
      241 ttaggtggta taattataac agaatttaat gatgatttta ctataaaata cactaacgct
      301 ggttatcttt caatgattgg atatacacgt gaacaattag aaaaggaact taaatacaaa
      361 gcttgtaatc ttatttttta tgaggataga aagaaggctc ttgaaagtat acaacatcaa
      421 ttaaacacag aaggaatagc tgtatatcaa catagaataa gaagaagaga tggtaatatt
      481 atctgggttt ctataaaagg aaggcttatt attgatgatg atggtattga aaaaggtatt
      541 tggattctaa atgacataac agatttaaaa aatgcagaac aaaaacttaa aataaatgaa
      601 gaaagattta gaatcgcatt aaagcaaaca tcaaacacta tatttgagta tgatataaaa
      661 acaaagtcac ttttaaatac tagtaaagag cttgataaat ataatattta taaaattact
      721 aaaaatgttc catattctct agtagaatca ggagttattc ataaagattt tagtgacgat
      781 tttataaata tgtatgaaaa tgtgataaat ggagcaaaaa aagcaagttg tgtagttaag
      841 atgaaacttc cagatgaaaa ttatatatgg aataaagtta ccttgactac tatttttgat
      901 gaagatggta atcctgtaag agcaattggt atacaggaag atataagcaa acaaaaggaa
      961 gcagaaataa gatacaatca agaaggtaag tatcattcag ctatattgtc ggaagcaatg
     1021 ataacttatg aaataaatgt atcaaaggat acttttatca aaggaaatga aaattggcaa
     1081 aaaccatttg gaattaagcc tgacaacaac tattctcaaa tggtaaaaat tatagcaaat
     1141 agtattgtac ataaagataa taaagaaaca tttttaaata catttttatg taaaaaatta
     1201 ttggatgcat ataaaaataa tgtaactgaa ataaaattag aatatcgaag aatgaatcaa
     1261 gaaaataaaa tgatttgggt catttgtaca atgcacttaa taaaagattc tattacgaac
     1321 gatataaaag cacttgctta tgttaaagat attgacaagc aaaaaaatga agaaattaga
     1381 ttaaaattaa aagctgaaag agacactctt acaggattat ataataaagg tactacagaa
     1441 aaattgatta atgatttctt gaactctgag gaagcaagct taggaatgca tgcattttta
     1501 attgttgata tagataattt taaatctgtg aatgataatt taggacatgt atttggagat
     1561 aaagtattat ttgaagtttc taaacaccta aaacctatat ttagaaaaga tgatatagta
     1621 ggtcgaattg gtggagatga atttatagta ttcttaaaaa atattcattc ttttaagaat
     1681 accaaaaaga aagcagaaaa aatatgcgat atatttagaa attcttatac ggaaaataaa
     1741 aagacatata aaatatctgg aacagttgga atttcatttg taccagaaca tggaactgta
     1801 tttgaagaac tttataaaaa agcagacata gcactttatt atgcaaaaag caaaggaaaa
     1861 gataggtatg cactttataa tgattgtatc caccaaaatg aacttgaaaa taatataagt
     1921 gataataata taaaattagc agataaatat gaggaatcca aaccagtact taaagaaatt
     1981 cttgattcta ttgataatta tatatatata gtgaatccaa atacttatga acttcttttt
     2041 gtcaataata aggtgtctcg tttggattta gatataaaat taggagaaac ctgttataaa
     2101 tcactattaa aatttgatag accatgtaat aattgtcctc ttaacggatt agaagaaaat
     2161 aagcattcaa aattcaaaat agagacaaat acatttggta aaaatatcag tatagcaagt
     2221 tggattaatt gcatagatgg agaatataac tgtcttatag agtgtataga ttcttcttta
     2281 tacaaaaata aataaaaaag gactaagaag gtatgttaaa tagtataact ttgatacttg
     2341 gtataatatt tacaacttac tatttttatc tcaaaatagt ttttggagga atttcattta
     2401 gtgagatttt cttagttgtg ggaattgtac taattatcta tcaaatattt aagaaaaaaa
     2461 tcaaagaaaa gaaggttttt tatagagtgt taaaaataat aatcagcatt tttttaatag
     2521 tatttgtagc tactgagagt ctaattattt tttatcctaa aaatagttta gaatcaaaaa
     2581 gtgattatct cttaatttta ggagcttctg taaaaaagac tacactaagt acaaccctga
     2641 agggtaggtt agacactgca ttgaaatacc taaaaattga tgataattgt tatgtggtag
     2701 tatctggtgg taaaggaagt ggagaaaaaa taactgaggc aaaagctatg aaagattacc
     2761 ttattaaaaa tggcatagat aagaatagga taatagaaga ggataaatcc actaatactt
     2821 atgagaattt taaatattca aaattgaaga tagaagaaca taatgaaaga aaactaagtg
     2881 atttaaaggt aaaaatagta acaacagatt ttcatgttct taggagcaaa atattagcat
     2941 atagaaatgg atataaaaat acgtcttttt atgctagtaa atcaaaactt tcatttgtac
     3001 caacttacta tacaagagaa ttttttgcat tatggaaaac aatcgttttt gatagataat
     3061 aaataaaaag actagatata aactcatatc tagtcttttc tattccctta attctaatcc
     3121 attttccatc tttacttgtt gtagaaattt caatattggg atttacaagt aattgcttat
     3181 aagaagtttt atgttttccg attccaaaat ataattttca ttcataatcc attacaaatc
     3241 tgaatggtct tactcttggt ttgttgtatt caactgtagc aaaatagact gtttagtttt
     3301 cttttaaaaa attcattatt ttttccatga ttaaatcctc tttgtatctt atactaatat
     3361 catgaatata aataaaatat tcataatact aatataaatc gacataaaaa caattacaag
     3421 attatattga ttattcaaaa ttatatataa aaatcagaaa aatattttag tttacataat
     3481 aaaaataatg ttaactaaaa ttgttgattt tagattaaag tgccagatta tgcatttaag
     3541 tagattttaa gcttgatttg attagaaagg tacaaactat cattgaaaaa aattaaaccc
     3601 atttaaaaat taatataaaa gcttgttatt tttgagaaaa atagactctc taaacttaat
     3661 taaataaaaa taaaatacag aaaattatat ctctgaatat cttaagtaat taagtttaaa
     3721 tttttgatat tttgtttgat gatttataat ataaaagcat attcttaatc tgctcaaatt
     3781 aaaaagagta aaaaataaca aaaaaatcac tgacatttca ctgacaaatt gtaattttgt
     3841 tattataaat acattgaaaa tacttgatta ttgaaaaatg gcataaaata atacatttta
     3901 tgccatttac tattatttga tagtttttaa cttatgacat aagatatttt ctcacatata
     3961 caataaaata taaaaaagtt gatattgcaa ataaaaaaat acatgttaga tgttatagaa
     4021 aaaagcgtac ttataggctt aaaatatcgc ataaaagagt ttttaaaatt atttattcta
     4081 attaaatttt aaatatatta aataatttta attttttata aaaatattca tatattttat
     4141 tcatattaaa gtttagatat ctaaaaattt ttttagaatt tattatatta tgttaatttt
     4201 ttatatctta taaggattgt atttttctaa ctataaaaat agtgttatac ttgtatttaa
     4261 aaataaaaga attttattcc tattgtgtaa agtgagaata ctattttctt atattggtat
     4321 aataatagta aatctataat atagaaaaga ggtatttcta gtatgcatga aaataaaaag
     4381 aatctattag acggaagttt agctcaatat aatgatacag cagttccaaa aataccagta
     4441 tttgctggta atgatataac ttcagaagtt tattatttta aacctaatca agttcttaat
     4501 gctcatagac accctaatgg tgaacaaata tttgtgttcc taaaaggaga aggtaaaatg
     4561 aaacttggtg agcatgaatg tgatgtaaaa aacggcgata ctgtatttgt accaacagga
     4621 gagtggcatg aaattactaa tggaagcaat gaagaaatgg tagccgttca aattacaaaa
     4681 attaatgctg gagcagaata cagaggatag atatataagt acagttagta ctttaatatc
     4741 tattttatta tataaacttt aatctcaaat ataaaaagag tcttttaaaa ggctcttttt
     4801 atatttttgt taattttcat tataaaaaca agaaaatatc tagctaaaac taatatattt
     4861 tatttaatat tttttatagc attgtttgca aactcagtat ttacgatatc tttataaggt
     4921 gcttccttat ctagttcttt agcttctttc ataacagtca tcaatttctt caaaccttcc
     4981 tcttttaata caggattttg agaccatgca tctattgatt tatatctatc tacaactgtt
     5041 gttaaatctt ctaaagatat atcttcaaag aaaggttgca tagccttagc tacttctttg
     5101 ctagatgctt gttgaatcca taattgacct ttataaacag catttgtaaa tctttgaatt
     5161 aaatctttat tgtttttcat gtatgatctc gtagtagagt atgctgtaaa aggtatttct
     5221 ccagaatctt ttcctataga tgtaactata tatccactac cttctttttc cattgctgtt
     5281 gctgtaggtt caaatgctgt tgtaaagtca ccctctccac ctgcaaaagc tccagccatt
     5341 acaccaaatt ggtaatcagt ctatttataa catttaaaac atataattaa tctcttggga
     5401 tattagggta aagtattaaa gaaaaatcat cagatttttg attaggttct tttttataga
     5461 ctgcgtaatt tattacactt ttaagcaata tattttttag ataaggttct ttttcctctg
     5521 tatattcact taaaattttt tcaagttgag gaacaatttg tgttttaact gtatttcttt
     5581 ccttttgcat ttcaatatct atatttgttt tattaatagc ttctcttgca ttttctatac
     5641 gagataagag tatttttaaa cgttccccaa atgtattact gtcataaatt ccttgttcta
     5701 aaaaatcatg tagtcgttct ttttgttttt ctaaagtatc caattcttta tttaaattta
     5761 tcaaggtatg ttctaaagta ttttcaggtt tagagttgtc tttttcatct tttaaagata
     5821 gtctataatt tactaaccat ttatttaaga cctctaatat tttattttct aatatgtata
     5881 gtttagtagc tttattttta caattagtac aaaccatata tttctcatat ccatttgatg
     5941 tagtattaga tttggctatt aaagtatgat tacagtatcc acattttaca attccagcca
     6001 atggatttgt tattttattg tttggagtag gggaattaat acgttttttt cttatatttt
     6061 gtgccttatt ccatatgtct tcacttatta ttggttcatg ctttcctttt acctcaattt
     6121 gttcctcaat tcctcttgtt ctactagaat ttcttttcct ctcgacttta ttccatacta
     6181 tatatccaca atacactttg ttttttataa tatctcttat acccttttca taccaagttc
     6241 tacctgttgt ggtcttataa ccaagttcgt ttaaataatt ggatatttta catgcaccat
     6301 ctccttttaa atacatatca aagattattt taacaacagt tgctttatct tcatttatta
     6361 caagtactct ttctttatta tttaaatatt taatatcata cccataagga ggagcttgag
     6421 ctataaattt accctcttca atacttttga tttttcctct ttgcatacgt ctagatatta
     6481 attttaattc cttacgagcc ataaaagctt caaattcaga atactcctca tcaaactcat
     6541 catttaaatc atacgttttt ctaggagtaa taattttcgt tttagatttt ttaaatgtat
     6601 ctagtattaa tccttggtct tgcatattac cacgacccaa acggtcaata tccatcacta
     6661 aaacagcatc gtagttatta tttttaattt cacctagtaa ttctagcatc ttaggtctat
     6721 attctatact ttccccagaa acaagttctt ctttaatttc aatgacattt aaattttgtt
     6781 ctcttgctag ttttaaaaga gttgtcttat gtctacttag agtttcaaat tcacctctat
     6841 gttctgcttc ttcatcagct ctgcttttgc gtaaataaat tgccacattc attaaatcac
     6901 accccttgaa tatattatat tcagtaacag attgattata tttaaaacac aaaaataaac
     6961 cttcaaaata caatatatat tttttaggtt tatttttata cagtaaattt tatagtatat
     7021 ttaaaagctt gtaatctaag taaatattta tactattaat tttatgcctt cttcattttt
     7081 taattttaaa ctattttttt actacaggta tccatacttc aaatttagca ttttgagggt
     7141 ctggatttaa gtacagttca atatcaggac cattatcata ttcataacca gaagtaggta
     7201 accattctgt tacaatacgc ttttctagtt cttgtattgc ttgtggacat tgtccttctc
     7261 cagaaaatat tgcccaagta aaagctggaa cagtgtattc ttctaatgta ttatctatag
     7321 attttgtact tgaaacagat ataaaatact tccattcttc tgaattatta caaatactta
     7381 tacctaatac accttgaggt tgactatcca tcatagggat taatttttga agcgttccat
     7441 ctaaagcaac cttttgccac atttggggaa caatttcaaa attcttttct agttcattgt
     7501 gaagtggctg agatacacca actatacgaa atgcttcttt cttttcaatt ctataattca
     7561 tttcttcggc tcctttaata ctaattttaa agctgattgg aggaaaggct tttaaagttg
     7621 ttatttctct tttttttata actgatgggg caacaccatg aatactttta aatgcacgat
     7681 tgaatgctgt tggagaagag tatccatatt ttaaagccac atcaattatc tttttatcat
     7741 cattttgtag ttctacagct gctaaggaca tccgtctacg acgaatatat tcagaaagtg
     7801 gtacatctgc catataagca aacattcttt gaaaatgata tgtagaacaa caagctattt
     7861 ttgccacttg ttcatactca atttcttttg ttatgttttc ttcaatatag ttaattgctt
     7921 tgtttaatct ttcaatccat tccataaaat cagctccttg taatttatat aatatctatt
     7981 ttaaaatagt taatcctctc tttctatgca ctaattaact agcttaataa aaaatgaaca
     8041 tgataaagtc tgtaattaac tacaaaaatg aacaaatagt attgtttttc acattaaagt
     8101 atatcacaaa ttagtatcta atttttatat aagataacag ttttaattta ttatgaaatt
     8161 tttataatat tttaggttag acatttaatt atatataaca ttttatttat agttaactat
     8221 ttttgaagtt tacccttaat caagcaacac ttagttattc aattataaat aatgattgag
     8281 aataggacaa tttttaagat acataaaata taaagaggag gggatttaaa tgccaaatat
     8341 agaaaaagac ttagatggta caactactat aacatacctc aacgacgata taggtgttcc
     8401 aacagacgag aatggatata aaatatctat gacaatacta catggaaaga caatctgcca
     8461 tgtagtatct cctgaaacaa tcttaggaag aaagttcaca ttagaagaaa aacaagaaaa
     8521 agatacaata atatatggag caatagaaaa aataatggta gacagacaat tacaaataaa
     8581 aaattcaaac aaagaagttg ctagtcatta gcacttcttt aataagtaaa gtaggacaag
     8641 tattttttat aatattattg acttgtagtc taattagact ataatataaa catagatagt
     8701 ctaattagac tactattaaa aataaataca ataagaattt aagaaaggat aataataatg
     8761 attaaatcat ttttaatgtt aggacagtcc aatatggctg gaagaggatt cataagtgaa
     8821 gtgactccaa tttataatga aagaatacag atgttacgta atggaagatg gcaaatgatg
     8881 acagaaccta ttaattatga cagaccagtt tctggggtaa gtcttgctgc atcatttgca
     8941 gatgcatggt gctgtgaaaa tcaagaggat agaattgggc taattccttg tgctgaggga
     9001 ggcagttcct tagatgaatg gaatatagat ggtatacttt ttaaacatgc tataagtgaa
     9061 gctaaatttg ccatacaaag cagtgaattg acaggaattt tatggcatca gggagaaaat
     9121 gacagtaata atggcaacta taaattttat tataaaaaat tactttctat tattgaaact
     9181 cttagaaaag aattaaatat tccagatata ccaattatta ttggtggatt gggagatttc
     9241 ttaggaaaag ttgtactgag tatgtattta tcaatcaaga gttacagaaa tttgcttttg
     9301 aacaagataa ttgttacttt gttacagcaa caggtcttac atctaatcct gatggtattc
     9361 atattgatgc aatatctcaa agaaaatttg gattacgtta ttttgaagct tttcataaga
     9421 aaaagcatat tatggaagca ttagctaatg aaagtgagtt gataataccg agtaattcca
     9481 agacatatac aaaaacagaa aaaatatata taaatagtat ggacttggca ctaggaaaga
     9541 tatcatatga tgagtttgaa tctaaactta ttaaacttaa tgataattag ataaaatatt
     9601 cattaatata ctaataaata tagattgaaa gaggtgattt tttgatacca ttattaattt
     9661 taggattatt aaaacaaagt ccaaattctt atggatatga gttattagca ttaatggaaa
     9721 agaagcatta caagtacata gtaaatttca ctaaaggttc tttttattat aatttacagc
     9781 aacttgaaga aaaaaagttt attgaaaaag ttgaaaataa acaatctaat aaaaacagag
     9841 aaactcataa ctacataatt actgaacttg gatgccagga atttgaaaaa ttaatgtata
     9901 agtatggttc taaaacagag tatataaatc tatcatttta cgcaacacta ttatttgcaa
     9961 atgattataa aaatgaaaag ttaatagaat tattggaaac gcaaattaag caaactcaag
    10021 taagaatttc tctattaaat gaatccatag aaaatgacaa aacaaattca cattatttta
    10081 atagaatgct agaaaattct cgttctcacc atttagttaa tattgaatgg tttgagaagt
    10141 tgctaaatga tattagagat tgattttaat aataaaaaaa catacatata tattttatat
    10201 aaatgtatga tatatagatt aaaaataagg cagataattt tcaattgaat atctgtcttt
    10261 ttgttatata agatgttaaa agaaacttat actatgtaag taaaatttat aaatataaag
    10321 ttgttcattg ttgaattaca gacacttaag atgaaaagta tgacatttga tataataaaa
    10381 ttgtttaagt attaattatg agtattaatt aatggtatag ataattttaa tataactgta
    10441 tttataaata attataaaat aataaattaa tttttttata ctgacttttt tatagtataa
    10501 atcgttatat ataatgacgg aggtgataat agtgaatgat gctcggatta tatcacaaat
    10561 aaaagcagga aaaaaagaat tgtttgatac tctaataaaa aaatactatg atagcatcta
    10621 ttgttattgt tatcgccatg tagacaataa aatgactgca caagatttaa ctcaagatgt
    10681 atttgtaaca gttataaata atatagaaaa ttataaacat tatggtaagt ttgaaaacta
    10741 tttatatgtt attgcaaaaa acaagtgtaa ggatttttgg aagaaaaaac aaccgatata
    10801 tttagagtca ttagataagg agatatcaaa tcaagaattg tctgatatag aaaataaaat
    10861 gtttataaaa gaagctttga ctaaattacc agaattagaa tttgaagtga taattttacg
    10921 gtattaccat gatttaaaat ttaaggatat tgctaaaatt atggagtcaa gcgtctctgt
    10981 cacaaagtac aggatgagta gggcattaaa aaaactagaa ttatatctgg aaggaggtaa
    11041 attatgaata ataaagaaat aaaaagaagt ctacagaagt ataaggttcc caaatatgat
    11101 tcttcagctc ttgaaaagac tattaaaact atacatgagt taacgcttag taatacttat
    11161 aaaagaatga ataataaaga cttttttata tctcaaattg gttttataag aaaaagaact
    11221 tggattatgc aatttctaat actatgtaca atatgtatat ttttatttag tacatcagca
    11281 gatagtaatt cattttataa tagtttatcc ttgatttcta tagcatcacc actttttgta
    11341 ctgacaaaca tagaagaatt aacaaaagta tataatagta gtattttaga gatagaattt
    11401 tcaacaaaaa atacattaca taaaattatt gctactagaa tgcttatatt tggaatgagt
    11461 gatttattgt ttatgattat tattttggta tctgcaaaac aaatcattga tgtaaacatt
    11521 ctgtatataa taatttatat actagttcct tttaacctaa tgtgtatagg atgtatggaa
    11581 attataaata gaaataaagg taagcataca aattattatt gtatgatata tggtatatct
    11641 cttgtattat taatagttac actgagtaac tttagagata atctatatca atttgaagca
    11701 ataaatcaat ggctaattgt ttttctagtc acagcagtaa cattaatata tgaaataaga
    11761 aaaacgtgga gacatctttg ttcatttgaa aatattatta tctaagtaaa aaataagaat
    11821 acacagtatc aggaggaata gtatggagtt aagtgtagaa tgtttaacaa aacagtataa
    11881 caataaaatt atagtagata aaattgactt taaattaaat gagggtatat atggattttt
    11941 aggagaaaat ggagctggaa aaacaactct tatgagaatg atttgcggaa tattaagtcc
    12001 tacatctgga gaagtaaagg tggatggtaa aaataatttg gatatgggag aagattttag
    12061 agatatgatt ggataccttc ctcaaaactt tggatactac ccagatttta ctgccaaaga
    12121 atttatgcta tatatagcat cattaaaagg attaatgcct agctatgcaa agagaaaaac
    12181 acaagaatta ttagaaatgg taggattatc agaggtaatt aataaaaaaa taagaacatt
    12241 ttctggaggt atgaagcaaa ggctaggatt tgctcaaaca ctacttaatg accccaaaat
    12301 tttaatatta gatgagccaa cagcagggct agaccctaaa gaacgagtta gatttcgcaa
    12361 cctaatttca gaattttcta aggaaaagat tgttatattg tctactcata ttgtgtctga
    12421 tgtagaatat attgcagata aaattttaat aatgaaaaaa ggaagatttg ttttaactgg
    12481 ctcaatagaa gaagtaactt caaagataaa agataaggta tggaaatgtg tggtcaatga
    12541 gaatgaaata gattatatgt ctgatagata ttgtttagca aatatccatc attctggtgg
    12601 agttgcaaat cttagaattg taagtgatat aaagccacat gacaatgcat gcaatttaaa
    12661 accaacacta gaagatttat atttatattg tttcaacgaa gtactataaa tatgcataag
    12721 gagataggta tgtctacatt aattaaattt gagttaaaaa aaataataaa aagaaagtct
    12781 acaatagcag ttattttagg aagtttattc ttaagcatca ttctttttgc attaatgcca
    12841 attcaggaaa aagctaatga tgaaagtgga aaagagtata ggggtttaca ggctattgag
    12901 ctaaaaaagc aacaatctaa attaaatgag ggatacataa cagataaaaa agtaaaaaac
    12961 tcaatcagag attaccaaaa aatgtttaat aatccaaata acattgtaac aaatgagtct
    13021 ggattctcag attttaagcc agatatttat tggaaattct tctatccaaa taaaactttt
    13081 tataatttta taatagagtc atatagtaaa ccaaatgatt ttaacccaga agtaattaaa
    13141 tcaatgccat tagaaaatga aattaatttt tataaattta gaaatgaaaa agtaaataca
    13201 atattaaata tggaatatcc agagggaagt tacacacaga aagaaaagga cttttggtta
    13261 tctaaaaata aggatataaa ggagccttat ttaaatggat attatcaagg gtgggaatct
    13321 tttctattgg caagagattt actgattttt actataatgg ctgtctgtat aagtatagct
    13381 tctgtatttt caggagaata ccaatctggt gctgattcag tagtattgac ttctaaatat
    13441 ggaaaaacaa aagttataac agctaaaata gtaagttcct ttttatttgg tacgattatt
    13501 tttacaataa atacaattgt gtctattggt atacttcttt tatcttttgg aattgatgga
    13561 tggaatttac cattacaact tacaaccagt tcaattccat acccatatac atttttacag
    13621 gcaatattac taagtgtgtt aattatttat atgatattaa tactattaat tagttttaca
    13681 cttttattat catcttatat gaaatcacct tttccagttt taattacaaa tataatatta
    13741 ctatttgtac caatattttt aaaatatagt gaaacaaata agttgtataa tcaaattctc
    13801 aatgttttac catcaaatgc tatttcattt tcatttacta attatgtaga ttataaattt
    13861 ggtaatttaa taatagcttt acctacaatg attattttta catgcttagt tcttacagtt
    13921 gtttttttac cttttattaa aaataatttt aaaaaacatc aagttaacta aaatatatat
    13981 tataaaaaga aggtgtctta aaatgaactt tttagcttat taggcactct tctttgtatt
    14041 aatttaaata tattttagta ttagattaaa tatgttttca tcattttaac aatgaaaaag
    14101 agccttatct ctattttgag atagctctta taaagttaaa tattaaattg ataactataa
    14161 aatatgtagt ttgaatttat aatttgtgtt ataatcatac tgatttttgt acatcagttc
    14221 ttacattaac attgcctgct tttagatttt ctccaatagt aagacctttt tcttttagaa
    14281 catattcaaa agtcattagt ggaactccac cttttctacc acctaatatt tcggttcctt
    14341 ttaaatcatt gtaagagaaa ttaggcattt ttttcctagc aactagaaaa ctaccatctc
    14401 tttttgtcaa ttgagcaaag ttcactgcat aatcgttttt gttttgatta tatacatata
    14461 tagatgcttc aggacccatt aaacctacgt ttgcttcacc tgaaatcaaa gctgccatag
    14521 ttttatctgc accttgggta tttataaggt caatctttat accttcatct tcaaagaatc
    14581 ctttcgtaat agcagcatac tgtggagcat aaaaaacaga gtgagtaact tctgctatag
    14641 ttactttatc cagattttta gacgaagaag attcatcttt tttgtcatta gagttaaaat
    14701 cacatccagt tagacatatt gtacttacaa tgatagagct tgctattgcg actatttttt
    14761 taaacataat aaatacctcc cataagacat acgaatagta tatagtatta aatatttatt
    14821 gtagttgtta caaaaatgta tctatactta taatatttct acaaaaactt aataataatt
    14881 ttcagtttta tgtttagttt tttcagtaat gtgtaatatt gaataaatta tattgattat
    14941 ttataaacta agtaataaaa ttaaaataat atcacttttt tgtct
//
LOCUS       DAEORZ010000102        14833 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEORZ010000102 DAEORZ010000000
VERSION     DAEORZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.102"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            80..898
                     /gene="larE"
                     /locus_tag="KRH39_002703"
     CDS             80..898
                     /gene="larE"
                     /locus_tag="KRH39_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBG8512592.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            953..2101
                     /locus_tag="KRH39_002704"
     CDS             953..2101
                     /locus_tag="KRH39_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBG8512593.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            2237..3625
                     /locus_tag="KRH39_002705"
     CDS             2237..3625
                     /locus_tag="KRH39_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG8512594.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            3654..4352
                     /locus_tag="KRH39_002706"
     CDS             3654..4352
                     /locus_tag="KRH39_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8512595.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            4355..5788
                     /locus_tag="KRH39_002707"
     CDS             4355..5788
                     /locus_tag="KRH39_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8512596.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            5810..6547
                     /gene="truA"
                     /locus_tag="KRH39_002708"
     CDS             5810..6547
                     /gene="truA"
                     /locus_tag="KRH39_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG8512597.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    6658..6865
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7118..8317
                     /locus_tag="KRH39_002709"
     CDS             7118..8317
                     /locus_tag="KRH39_002709"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBG8512598.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            8564..9610
                     /locus_tag="KRH39_002710"
     CDS             8564..9610
                     /locus_tag="KRH39_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBG8512599.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYIKTNKADLKRMVALGDGRTKQMYEFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DGGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTGQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            9718..10881
                     /locus_tag="KRH39_002711"
     CDS             9718..10881
                     /locus_tag="KRH39_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8512600.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     NRNHSSTDLQEIAKEITDISKKDYEILSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(10970..11350)
                     /locus_tag="KRH39_002712"
     CDS             complement(10970..11350)
                     /locus_tag="KRH39_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBG8512601.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(11353..12012)
                     /locus_tag="KRH39_002713"
     CDS             complement(11353..12012)
                     /locus_tag="KRH39_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBG8512602.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(12118..13092)
                     /locus_tag="KRH39_002714"
     CDS             complement(12118..13092)
                     /locus_tag="KRH39_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG8512603.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGILVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDIAGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVKGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKTINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(13132..13479)
                     /locus_tag="KRH39_002715"
     CDS             complement(13132..13479)
                     /locus_tag="KRH39_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512604.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(13697..14653)
                     /locus_tag="KRH39_002716"
     CDS             complement(13697..14653)
                     /locus_tag="KRH39_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBG8512605.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDIDPIRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
BASE COUNT         5802 a         1603 c         2418 g         5010 t
ORIGIN      
        1 aaataaatac tttatatgtt ataatcatta agaatgtaaa aaaataatat atagttttat
       61 agttcatatt tttccagata tggaggtaaa ttttgtgaag gaaaaagaaa aattagataa
      121 attaaaaaaa atgttacttg aattaggcag tgttgtagtt gcttattctg gtggagtaga
      181 tagtaatttt ttgcttaagg tcgcaaaaga tacattaggt gaaaatgtta tagctgtgac
      241 tattcatgca atgatgcatt caagtaggga aattgaagag gctaaacaat atactcaaaa
      301 ttttggtgtt aaacatatca tattaaatat agaaaacttt gatttaaaag aatttaaaga
      361 aaatggtgtt gatagatgtt atcattgtaa aaaatatata ttctctaaaa taaaagaggt
      421 agcaaaagaa cataatataa agtacatagt agatggaaca aatatagatg atttaggaga
      481 ctatagacct gggcttaagg cattgagtga attaggagta attagtccat taaaagatag
      541 tagtttaaaa aaagaagaaa ttaggtcgct ttcaaaaaca ttaggattaa atacttttaa
      601 taaaccatca tttgcatgtc ttgcgagtag gataccttat ggagttgaaa taacagatga
      661 aaatttaaga atagtagaaa agagtgaaga atatctatcc aatttaggtt tttctcaatt
      721 tagagtgaga atgcatggag atatagctag aatagaagtt ggacaggaag agctaggtaa
      781 attttttgaa aataacaatt ttaataaagt tgatactaaa ttaaaaatat ttggctttaa
      841 atatgttaca ctagatatgt caggatataa aatgggaagc atgaatttaa atgtgtagat
      901 tattttaaaa ataaataagt tttatagtta atataaggaa acaggaggtt ttatgaaaaa
      961 ttatacgtta atatcgccat gtttttttgg aatggaaaaa atgctggcta gagagattac
     1021 taatttagga tatgaaataa taaaaacaga agatggaaga ataacatata aaactgatga
     1081 gtttggaata gcaaagtcaa acatgtggtt gagatgtgct gaaagagtac atcttaaaat
     1141 tgctgaattt gaggcaaaaa gctttgatga attatttgaa aatacaaaaa gaataaactg
     1201 gtctagatat ataccttatg gagctcaatt tcctatatca aaagcttcct ctataaaatc
     1261 aaaattatac agtacaccag atgtacaagc catagtaaaa aaggctatag tagaaagctt
     1321 aaagaaaagt tatttggagg atggtttgct taaagaagat aaggagaaat accctatttt
     1381 tgtatttata cacaaagata aagtaaccat ttctatagat acaacaggag atgctttgca
     1441 taagagagga tatagagaaa aggcaaataa agctccaata agagagactt tagctgctgg
     1501 gcttatttat ttaactccct ggaaagcagg tagagtttta gtagacccta tgtgtggttc
     1561 tggtactata ttgattgaag ctgctatgat tggtataaat atggcacctg gcttaaatag
     1621 agagtttata tcagaaaagt ggagaactct tgataagaaa atatggtggg atgtaagaaa
     1681 agatgctttt aataagatag ataatgaatc taaatttaag atttatggat atgatataga
     1741 tgaagaatct atagatatag ctagagaaaa tgctgaaata gcaggtgtag atgaatatat
     1801 agagtttaat gtaggagatg caactcaatt taaaagtgaa gatgagtttg gatttataat
     1861 cactaatcct ccttacggag aaagattaga agataaagac agtgttaaac agttgtataa
     1921 ggagcttggt tatgcattta gaaaattaaa aaattggtcg tattatttga taacttctta
     1981 tgaagacttt gaatatgagt ttgggcaaaa agcagataaa aaaagaaaat tgtataatgg
     2041 tatgttaaaa actaactttt tccaatatcc gggtcctaag cctcctagaa ataataaata
     2101 agttttacat gatttatttg taaaatataa aattttatta aaataagtaa aacattatga
     2161 tgccctgaaa cgtctaatta ttgtgaggag atttttgtga acaaatttaa agatttatag
     2221 gattaggagg agttaaatga aagtgttaaa gcttattact gtagtagccc tgatgatgtt
     2281 atttgttact ggatgtaata taaaaactga atctccagaa caattagcga agatacctga
     2341 ctatgatgat acaaaaaaag tattgtatga tggtattgac cagttactaa aaccagattc
     2401 atctatgata ttgccttcta atacaaaaga agtaggaaaa ataaataagg tagatttaaa
     2461 cagcgatgga attgatgaat tagtagtatt tgaacaaaaa gaagatttaa gtaataatgt
     2521 gagtcaggta gggtttgtaa ccttgagtta caatggagaa aaatacgtac ttggagacca
     2581 ttttttagaa aatggcgaat caatagaata tgctaatttt tatgatttgg actcagatgg
     2641 atataaggaa gttatcttat tggttaaatc caaagacaaa acgaatatgc atatatataa
     2701 agtagtagat aatgaaataa caaaaatata tgacttggat gcatcatggc ttcaaaataa
     2761 agaagatttt aatgacatga aagtaaaaat tggatatata gatggagatg acaagttaga
     2821 catattgatg cttcatttga ataataagac taatgaaatg tttgcaagtg ttgcaaattt
     2881 tgatgggaaa atgaaaatca aagattctgt taagtttgaa aatgtaaaaa acttaagtga
     2941 attatatata acattaggta atgttgcttc atctgttgga aattcttctg cagctataaa
     3001 aggtattgta cttgatattc ctatattgaa agataataac tatattacgc agatacttta
     3061 tatgaaagat gggaaaatta acaaagcttt tagtgattat gataaaacta ttactaaatc
     3121 ttattatata cctgtagatg atattgacaa agataagatt atagaaatac ctatagttgc
     3181 aggcagtaca ggtaataata aaaatactta tagttctaaa tcatctgcaa ctataagttg
     3241 gtatagatgg aatggaaaag aaggtgctga ctcaagttta atatttacaa gtcagatata
     3301 ttataattat aaatataatt ttaaattatt tattccaaat aatttatttg ataaaatact
     3361 tgttgaacag gaatttgttg gagaaaaagc agtatttaaa ttttactatt ttgactattc
     3421 agaccgtaag aatttattta atatagtagt agagagtaaa aataaacttg aagatagaaa
     3481 aaatacaggt actcaaaatt ctattgtact tcaagagagc gatgaatata ctttcttact
     3541 agttgtaaac aatgcaaatg aaatggataa gctagatatg actatagacg cattaaaaga
     3601 atatttctca ttaatatatg gttaatatta tactcaattg gaggttttat tttatgaagg
     3661 aaaagatact tatactagaa gatgaaattg gtattagaag ttttgttagt ataaacttaa
     3721 aaagggaagg ctatgaaata gttgaggcag gtactggaag agaagctata gaaaaaatga
     3781 caacagaaaa ggatattaca atagctcttt tagatgtaat gcttccagat ataagtggta
     3841 tagaagtatg taaatttata agggagaatt ttgaccaagt aggtataata atgcttactg
     3901 ctaaagctca agaagatgat aaaatagaag gatttatctc aggagcagat gattacatca
     3961 taaaaccatt tagtataaaa gagcttttag tcagagtttc tgctcttctt agaagagtag
     4021 caaaggatga ttcaagtgtt aagtcgagtg agatagtttc tccaccattt atacttgata
     4081 tagacaaaag aaaattattt aaaaatggaa aggaaattga gcttacacca actgaatttt
     4141 caatagtgaa atatctaata tcaaatgcta agcagtcgtt aagtagagac cagatattag
     4201 acgaagtatg gggaactaac tatttatatg attttaaaat agtagatgta aatataagaa
     4261 ggataagaaa taaaatagaa gatgacccat caaaaccaaa atatatacaa actatttggg
     4321 gttatggata ctgttttaga aaggaagaat aatagtgtta cattttgaag gtctgagaaa
     4381 aaaagtaata aaaaactatt ttataataat tattataatg gttactctgt ttgaaggatt
     4441 gttcatgttt tacatacaaa actactatta tgattctgtc aaacagctac ttgaatctga
     4501 aataaaatat gctgatgaat ataatgctat tacaatggaa actacaagtt ttgagaaaaa
     4561 agtaaaaaat atatttgata aacaaccctt aactaaaaat tcagaatttg gaatttcaat
     4621 aatagataaa gataaaaata ttatattaga ccaatatggt tttaaaagta aagaaaaagc
     4681 aaactatgaa gatgttaaca atgcattaaa agatataaaa actaaaaatt taacacctta
     4741 tacttataga ataccagata caggggagca tgttatgagt atctccttac cacttaaggt
     4801 aaataatata atagaaggtg ttgttagata tacagtatca ttagatgcta ttgataatgc
     4861 aatattaaag caagctacat ggcttatatt agcaggaata tttatactta ttatagctat
     4921 actaataagt cttaaatttg ctgagactct tataaaacca ttacgagagc taaagaaatt
     4981 tgcaaatgaa ttggctgtag gaaattataa tataaaatta gaaaagatga agattgtaga
     5041 tgatgaaatt ggtgatttag cacagacatt tgaacacatg gcacatgaga ttgacaaaag
     5101 tgaaaaatta aaagaggaat ttatatcatc tgtatctcat gaactaagga ctcctcttac
     5161 atctataaag gggtggagtg aaaccttagg ttatgaaagt ataacaagag aagaactaga
     5221 cttaggttta ggcattatac aagatgaaac agaaagactt ataaaactag ttgaagaatt
     5281 attagacttt tcaaggcttt cttcagacag gataaaactt catgttgata tagttgatgt
     5341 tgaaggttta attgtaggtg ttgtaaatca acttaaagtt aaagcagctg aaaaagatat
     5401 atcactttta tttgaatttg aaaatgaatt tatagagaac attcaaggag ataaaaatag
     5461 attaagacag gttctcataa atttaataca aaattcattt aaatttacga gtcaaggtgg
     5521 ttacataaaa gttgtagctt ctcaagatga agagataaca accatatcag tagaggataa
     5581 tggttcaggt atagaaaagc aaaatttaaa taaagtgtta gataaatttt tccaagaaga
     5641 ttataataag gctggtagtg gattaggact agcaattagt aatgaaattg taaaacttca
     5701 tggtggaaga atgaaaatag aaagtgaaaa gaatgttgga acaaaaataa cttttaatat
     5761 aaaaaataaa tttgctaaac aagcataaat tcttagaaag aggattaaga tgagaaacat
     5821 aaaaatgata gtagcatacg atggttcaag atataaagga tatcaaaaat taggtgacaa
     5881 taatatgact attcaagaaa aacttgaaaa tgttttaagt aaaatgacta atgaaacagt
     5941 ggagataata ggttctggaa gaactgatat gggagctcat gcaagaggac aagtggtaaa
     6001 ttttagaact aattgtatgg attctttgga taaaattcaa aaatatttat atgaatattt
     6061 accagaagat atagttgtaa aaactgttga agaagttgat gaaagatttc attcaagata
     6121 caatgtaaaa tcaaagactt atatgtataa aattgacaat aataagtatc ataatccttt
     6181 tataagaaaa tatgctactc atgtaagtaa aaaattagat ttagatagaa tgagaaaagc
     6241 aagtgagtat ttagtaggag aacatgattt tactagtttt gcttcttcta aatctaagaa
     6301 aaaatcaaat gtaagagaaa tttattctat aaatataaaa gaagatgata atgttataga
     6361 aatatatgta gaaggaaatg gatttttgta taacatggta aggataatag taggtgcttt
     6421 gatagatgtt ggtctaaaaa gaagagctcc acaagatatt aagtatatgc ttgaatcaaa
     6481 agatagatgt caatcatcag atactgcacc agctaaagga ctatgtcttt ggaaagtaac
     6541 atattaatca attatataaa actctcttta aaataaaaat aaaataaatt atatagttga
     6601 cataaatgga aaaataaact attatatatt ttataagaga gatataaaac tttatattta
     6661 aatcctgtga gaaagagagt aacttctatg tgttttatag agagctgagg atggtggaag
     6721 cttggcaaat aagtattagt gaaaagaact ttggaggata ctaattgaat ttagtatgtt
     6781 agtaggtaat ctcgccttaa gagaaagagt ggataagttt atcttatcaa ttagagtggt
     6841 aacgcggata tatttcgtct cttggttttt ccaagaggcg ttttttaata tatttaatta
     6901 ttagtgttta tctggtatgt tgaacattta ctaattgatt agctaagttt atcaataaga
     6961 gtggtaccgc ggatatttcg cctcttagta ttttaaaata ctaaggggtt tttttagtat
     7021 taaaatgatt ttatgtgagt ctaaattctc agagtcattc aatattaaga atttctataa
     7081 aataaaaatt ataatttaag attgggagga attaaaaatg aaggaaaaag tagtattagc
     7141 atattcagga ggacttgaca catcaatcat aataccttgg ttaaaggaaa attatgaaga
     7201 tatagatgtt atagctgttt gtggaaatgt aggtcaggaa gataaaatgg aagatgtata
     7261 tgaaaaagct ttacaaagtg gagcatcaaa ggcatatgtg gatgatataa gtgaagaatt
     7321 tgtaactgaa actatattta aagccgtaaa agcagaggct aaatatgaag gaaaatactt
     7381 attaggaact tctttagcaa gacctataat agctaaaaaa ttagttgaag ttgctcataa
     7441 agaaggtgca aaatatatat gccatggatg tacaggaaaa ggtaatgacc aagttagatt
     7501 tgaagctaca attgcagctc ttgacccaac tataaaagtt attgcacctt ggagaatttg
     7561 ggatataaaa tcaagagaag atgctataga ctatgctgaa aaacataata taaaggtaac
     7621 agcaactaaa gctaaaatat attcagtaga tgccaacctt tggcatgttt caacagaagg
     7681 tggagatata gagcacctag aaaatgaaca taaaaaagat gtttataaac aatgtgtaga
     7741 cccagaagat gcatgtgatg tagctgaata tgtagaagtt tactttgaaa aaggtgtacc
     7801 taaaaaggta aatggcgaag aattatctcc agttgcactg attcataagt taaatgagtt
     7861 gggttgtaaa catggaattg gtgttataga tatagttgag aatagacttg ttggtatgaa
     7921 atcaagaggt atatatgaga ctccaggagg aacaatcctt tatgaagcac ataatatttt
     7981 agaaagtgct actttagaca aagatacatt acatttcaaa caaatggtat cttataaata
     8041 cggagaactt atttacaatg gactatggta ttgtaaatta agagaatcta tagatgcatt
     8101 tatggaacaa actcaagata atgttacagg aacagtaaaa gttaaacttt acaaaggaaa
     8161 tataaaacct gctggaatat ttactgaaaa tgctctttat gatgaaggaa tatcttcttt
     8221 tggaaatagt gaactttatg accacaaaga tgcagaagga tttattaatt tattcacttt
     8281 accattaaaa atcagagcta tgaaagctgg aaaataataa aataaattat ttaagagtgt
     8341 ataaaaacta tttttaggtt tttatatgct ctttttttat ttgaatttat taaattatat
     8401 agtaaatagt ataaaaaaat atattatagt tccaaatggc ttaataatgt gaaaatataa
     8461 gacctaaaat ataaatttta taaatacaat agttattgac tctctcattg taagagggtt
     8521 tatactgaat ctaatgaaaa tttatagtga ggagatagat tatatgagaa aaataattga
     8581 aattaaacat cattcacatg attatgagtg tatgtggaat ggtatagaag atttatatat
     8641 aaacaaaaca ggtgaatgcc ttccaaatga ttttttcttt ttactatcag gttttggttc
     8701 gttttgttat attaaaacaa ataaagctga tttgaaaaga atggtagctt tgggagatgg
     8761 aaggacaaaa cagatgtatg aatttttagc accaattgtt ggctttgaat acaaacatca
     8821 tgagtttaaa aagtatgagc aagcattaaa gaaagcaaaa tctgaaattg atggtggata
     8881 tccagttatt ttaggagctt tagatatgta ttatctacca tattataaga agctatatca
     8941 taaagaacac attccttttc attatatatt aatggtagga tatgatgatg aaggaatata
     9001 tctgtatgat tgtggtagaa cagaattatt acatctttct tatgataaat tacgtgaaag
     9061 catgaattgc aattcccctg gattaagcaa agaaaatact atttgcacaa taagaatgaa
     9121 tacttcaaag aataaatata acattgctag agaagctatt gctataaagc gtgatatgtt
     9181 tttaaatcca cctaaaggat ttttgggata taaaggtttt gaaaagttta ttaaagagtt
     9241 gccacagtgg aacactgaat tggataaaga tgattatgat aagattttga ccaatatggt
     9301 tatgtttttt ggaacagtac caacaattcc aaatgcaatc aaaggaatta aagaaccaga
     9361 tactgttcaa tttaaaggtg gatttgataa aatgagtaga atgctcaaca gtattgggga
     9421 ggaatacaaa aacgactctt ttatacaagt agctgatatt tttgataaaa ctgctattat
     9481 aattgagaaa atatccaata ttataataga ttacttaact caaacatgtg atgatacagg
     9541 acaacttccg ttattgttct ctgaggtctt agagcatatg aaaacaggat atttaatctt
     9601 ggatgtataa aaaatatatt gttgacccta acattacgta atagtgtaat atgaaaatat
     9661 agcttgaata tattagtagg tattgatggc tgtaaaatta aaatttgagg tgtggatatg
     9721 agaacagtga aacaagtttc tgatttaact ggaataagtg tgagggcatt acattattat
     9781 gatgaaatag gtcttttaaa accaaataaa attacagatg caggatatag actttatgat
     9841 gatgaatcta ttaaaactct tcaacaaatt ttatttttta aggagattga tattccttta
     9901 agagaggtta aggaaataat gtcaagccaa tattttgata aggttgaagc tctaaaaaat
     9961 caaaaaaaat tacttatttt aaaacgaaag agactggatg aattgataga gcttataaat
    10021 caaactctaa gaggagaagg aaatattgat tttaaagagt ttgatatgag cgagtatttt
    10081 gatgttttag aagaatttaa aagagagcat aggaataagg taattaaaat ttatggtagt
    10141 gtagaaaaat acaatgaata tatagaaaga gttaagtcta atgaagagaa aattgctaaa
    10201 atggctataa aacaatatgg aactattgag aagtttgccc aagctataaa aacaaatttt
    10261 agcagtgata tattgaattt aggagaaaaa tttgatagat ataaaaatga ttgtttaaaa
    10321 gaaaaacatc caaaattaaa agaattatat agaaagcttg tagaagattt aaataggaat
    10381 cattcttcaa cagaccttca agaaatagca aaagagataa cagatatatc taaaaaagat
    10441 tatgaaattt tatctatgga tactggtgat gacaactggt attatatggt tcaaaattat
    10501 ctagtaaatc ctatgtggat agaagaagta gataaaaagt atggaagtgg agcaggtaag
    10561 tttattggac aagtactaaa gacttatcta agagatagaa aaccaaagat aaatacattg
    10621 tatgaaaaac ttgtagaaga tttgagtaga gattgttctt caagggaagt tcaaagcatt
    10681 gttgaagaaa tagacaatga aatgaaaaga agtaatgaat tttataaaat agacaatggt
    10741 gaaagatact ttgattatat gtcagagctt tatttacaag attctaacta tataaaagta
    10801 actgataaga attatggtga tggtgcatct aagtttattg gagaagcttt taagatttat
    10861 tttgataata ataattgttg aaaatttgga agtaaaaaat atgtaagttt tataaaagca
    10921 caaaaataag gatgtagatt agctccatct atatccttat ttaaagttat taaaatatat
    10981 agtttacatc aaacttaaca ctttcaataa tatttcccaa atatgttttt atactttcaa
    11041 cttcttctaa agattttaat ttattttcag tataaattgc tttgtccaga atattaaacc
    11101 ttaatcctat ttcatcaaag tactcatgat ttaaaacttt gtaccaatgg tttttttctt
    11161 tattccaact cgtagatatt gaatcagctt ctttttgagc tttttcccaa tcttcatttt
    11221 ctatagattt ttctaaaaca gatatattgt ctttatatcc ttctgtaaaa tcatatagtt
    11281 tattgttaac ataaaatcca aataatacaa ataagatagt ccatagtata acaaatgttg
    11341 tagatttcaa tattaatcac cctttttatt ttgaataaat attttgtcat tttcatctaa
    11401 aacacaaata agaacatctt taaaagtttc tatgtgatta gattttaaaa tacccattaa
    11461 ccagttagta tctttttgag ctatgtctag atttttattt attattctac catccagtat
    11521 aagtgggata ggtaaatgat taaaagctct tggaggagta ctattgtagc ttgatgctgg
    11581 tacaacactt agattaccat cagtctccaa aatagcatat tgtacatctt ttaggttaaa
    11641 gtatccttga atcctaagtt gttctaataa ttcatctata ctaattcttt cttttttaag
    11701 ctctttatat acaatttttc ctttatctat taatacagaa ggtttacctg ataaaaatga
    11761 acttaatttt ctacttttta atgataagaa tgaaattaga gtctgcatga ttacaagtcc
    11821 agatattgct gcaacaccat ttatgattgg tatattatta ttttccattg ggacagatgc
    11881 aacttctgca attaatagtg ttataactaa atcaaaacag ttcatttcag ctatttcacc
    11941 ttttcccatg actctaagtg caattagcac tgttatgtat aatataatac ttcttattaa
    12001 tactactgtc atatctatca attactcctt taaaaaatat ttacacttag ttagtatatc
    12061 ctatataaaa aatttaattc aaagtataaa aaaccttaaa taattggatt caactattta
    12121 aggttttttt gataatttta atattaaatt ttttatggat tttctgttac ttttatttat
    12181 tttaataaca gattcaacaa aactcttttt atctcctaat tttaagataa aatcaggata
    12241 ttcttcaaat tcattgagtt ttattaattt gaaagaagaa gatagatttt taaagttaat
    12301 agttttagag ttattcacta atacacttct ttttttaaaa taaatttctt ttttacttat
    12361 atttagtata gttaatattt gtttaagata tattattata tcttctggag ttctatcaaa
    12421 tttgagttca aaattttcat cttcaaagta gggaatataa tataaaggtg ttttgagagc
    12481 aggtaattta ttatataatt cttcaagaat atcgatattt ttaggggagt taaagaaaaa
    12541 gcatttacat acattcatta aatccacagc atctgaaagt gagttatgtt taatatctcc
    12601 ttttacagaa aaaatttttt tcatattcag taaagatatt tgcttagata agattctatt
    12661 attgtatgta atatgttgag atataaaagg ttgaatgtca atcatcatat taaataactc
    12721 taagcattta cctgtatatt tatacactct aaatgaagtt agaaggccag ctatatcaaa
    12781 atcaccccaa ttatatattg taacatcatc aaatttacaa aaccatttat aaaaatcatc
    12841 cataacatat aaaagattag gagcattatt gatatctagt tggttcaatt tggttaaatt
    12901 tttacattta tttgaaagta ttttattgta ttttggctta actaaagagt aataattatc
    12961 tattgtatta aattcgttat caactaaaat tccaccaata gaaattaatt ccgcaccatt
    13021 atattctttt ggtttatttt tattatcatc tagtatatta aattcaaagt ctataaataa
    13081 cttgtatttc ataaatctaa agcacatcct caggtaaatt atttattgag cctaagcatc
    13141 ataagaatca atagatataa tgactctaaa catcaaaggt tcattacaac ttttatttat
    13201 ttcataattt aattcaaata aatcttttaa gatataattt ataagtattc tttcccaacc
    13261 catttttaaa ttaactttag agtctattaa tatattttct atctttttaa atatttctat
    13321 atttgctggt attatactga gctcaatatt acttttaaac gggttagact gtaaatctgt
    13381 tataagtata ttttttaatt cctctgtaag ttcagaaatt aaactatcac ctatgtattt
    13441 ttttatgatt tcctctctaa tttcttttaa atttagcaat attagtacct gctacttaca
    13501 tagtagcttt tcctttcctt ttaatactat tattcatttt tgaatcttat ttagatatat
    13561 tttaagatta aaagtcttta gctatataat tttttaccca tttttaactt atattaacat
    13621 attacaggct tatatctcca ccctgcttac attgaggatg gagtatattg cctattttga
    13681 taaaaatgat aggttgttat ttgcctattc ctttttctga taatttttct aacatatctt
    13741 tggtcatttt atctaaatca taactaaact taaagttcca ttcttttact gcactactac
    13801 tgtctagaga gttaggccaa gaatcagcta tactttgtct tatagggtct atatcatatt
    13861 ctatcacaaa atcaggaata tattttttta tagaatcagc tatttcttca ggagaaaagc
    13921 tcatagcagt aatattaaaa gcatttctat ggatgagttt atcagctgga gcttcgatta
    13981 aatccactat tgattgaagt gcatcaggca tatacatcat atccattttt gtaccttctg
    14041 ctatgtaaga tttatatctc ttatttttta atgcctcata atatatatca actgcataat
    14101 ctgtagttcc tccaccagga ggcgtaacat aagaaataag acctggaaat ctaacacctc
    14161 tagtgtctac tccaaatttt gagtgataat aatcacatag taattctcca gaaactttag
    14221 tcacaccata catagtatta ggtctttgta atgtatcttg aggagtcata ttttttggag
    14281 aattttcacc aaaagcagca attgaactag gtgtaaaaaa tttacaatcc aattctttgg
    14341 ctatttcaag accatttatt aatccattca tatttaagtt ccaagcttct agaggtttac
    14401 tttcagcgac tgcagaaagt aaagatgcta gatgaattat agtatctaca tcatattttt
    14461 ttgcaatttc aaaaaaacga ttcttatctg taacatctaa tatctcaaat ataccagatt
    14521 cacatactgg attaccttct tttactctcc ttgaactagc gattacattt tgttctccat
    14581 actcatttct aagctttatt gttaattctg aacctatttg tcctaatgca cctgttataa
    14641 gtattttttt caattttaca acctcctaag ggtttaattt atgtagtact acttattttt
    14701 attttagcaa atcttttgtt taaatacaaa gaattaaaga taataatata cgacttaatt
    14761 gaatgaagtg cagtaagtgc ctaaaaattt aaagctaata tataatggtc tattgtataa
    14821 caataaaaga gta
//
LOCUS       DAEORZ010000103        14828 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEORZ010000103 DAEORZ010000000
VERSION     DAEORZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.103"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            562..1473
                     /locus_tag="KRH39_002717"
     CDS             562..1473
                     /locus_tag="KRH39_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG8512606.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1648..2145
                     /locus_tag="KRH39_002718"
     CDS             1648..2145
                     /locus_tag="KRH39_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG8512607.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
     gene            2889..3251
                     /locus_tag="KRH39_002719"
     CDS             2889..3251
                     /locus_tag="KRH39_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG8512608.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            3316..3933
                     /locus_tag="KRH39_002720"
     CDS             3316..3933
                     /locus_tag="KRH39_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBG8512609.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLKEILVRLGLEV"
     gene            3930..4415
                     /locus_tag="KRH39_002721"
     CDS             3930..4415
                     /locus_tag="KRH39_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBG8512610.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            4458..4976
                     /locus_tag="KRH39_002722"
     CDS             4458..4976
                     /locus_tag="KRH39_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBG8512611.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKIIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            5112..6071
                     /locus_tag="KRH39_002723"
     CDS             5112..6071
                     /locus_tag="KRH39_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG8512612.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            6097..7800
                     /locus_tag="KRH39_002724"
     CDS             6097..7800
                     /locus_tag="KRH39_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBG8512613.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            8013..10115
                     /locus_tag="KRH39_002725"
     CDS             8013..10115
                     /locus_tag="KRH39_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBG8512614.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            10128..10568
                     /locus_tag="KRH39_002726"
     CDS             10128..10568
                     /locus_tag="KRH39_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBG8512615.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            10604..11407
                     /locus_tag="KRH39_002727"
     CDS             10604..11407
                     /locus_tag="KRH39_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBG8512616.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            11447..12043
                     /locus_tag="KRH39_002728"
     CDS             11447..12043
                     /locus_tag="KRH39_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBG8512617.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            12149..13012
                     /locus_tag="KRH39_002729"
     CDS             12149..13012
                     /locus_tag="KRH39_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBG8512618.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMVVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            13079..14068
                     /locus_tag="KRH39_002730"
     CDS             13079..14068
                     /locus_tag="KRH39_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBG8512619.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            14160..14771
                     /locus_tag="KRH39_002731"
     CDS             14160..14771
                     /locus_tag="KRH39_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG8512620.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         6077 a         1374 c         2750 g         4627 t
ORIGIN      
        1 atagagttga gaaatagtaa attgaaattt taaaataaat gagtggaagt agcttagtaa
       61 agtatggaaa tgaatattac aataaaaaat aaataatatg taaagacttt aaattaattt
      121 taagtattaa gtacaaatac taatttttga tatagaaatt gaataaagta aatgataata
      181 agcaaatgaa ttaagatgat aataagtgaa ttgatataga aataagtaaa ttaagtaaga
      241 cgataacaag tgagataata ataaatgaat tgacatagca ataagtaaat taagtaagac
      301 gataacaagt gaggtaataa taagtgaatt gatatagaaa taagtaaatt aagtaagacg
      361 ataacaaatg agatgataat aagtaaattg atatagtaat aaataaatta agatgataat
      421 atccaaagtg agaaagataa tagaaagtaa ataaaatcat gtaagaataa tttagataag
      481 attctaagat tttatttatt taaaaattag tatgattaat ttagattaaa taaatttaat
      541 aattttagta ggtggtaatg aatgaaaata aatagattaa cagaaataat agtaatttta
      601 ttaaataaaa aattagtaac agctaaagag ttggcagata ggtttgaagt ttctacaaga
      661 accatatata gagatataga aactctgtct atgtcgggag taccagtata tatgacgaaa
      721 ggcaaaggag gagggatatc tttaatagaa gaatattcta tagacaaggc catattgtca
      781 aaaaaagata aagagagttt aatagtggca ctgaaaacat tacaggcgac taagtatcca
      841 gaaataaatt cagtagtaaa taaaattggc tcaatatttg gagagcaaaa tttttcaaac
      901 tggattgaaa tagactttac tgaatgggga agtaatttta atgaagatga taaatttaca
      961 aaaataaaag aagctatttt aaggcgtaat actataaatt ttaattatgt aaatagttta
     1021 tcaagccaga caaacagaac tgtcgaacca ctgaagctta tgtataagag taagacttgg
     1081 tacttgtatg gattttgtaa actaaaagat gattttagga tatttagaat tagtaggata
     1141 aggaatttat ctataaagga tgaagtattt tcaagaaaga taatagaaga agtatgtttg
     1201 aatgattcta aagtaattaa ggaaaatact ataacgttga aattgaggtt taaagaaaag
     1261 atgcttttta gagtatttga tgattttaat aaggatttaa taactaaaaa tgaagatgat
     1321 acatatgatg taattacaga atttcctatt ggtgaatgga tttatggata tattttatca
     1381 tttggtgaca atgttgaagt acttgaacct aaagatgtta gggataatgt aataactaga
     1441 ctaagggagt tgtctaaaat ttattcatta taatataatg ctagattaaa gattttatta
     1501 aatatataat atcttgcatt tagtatataa tatctcactg agtttaatta tattaaaaat
     1561 atgacataaa ggtgtcgtat attataaatt ataattgaat tagttcaaaa taaaatagat
     1621 taataaaaac aaagtgaggt ataaagtatg aattatgaaa tagtagagat aaatgagaaa
     1681 atagttatag gaatatcgaa ggagactaca aataaagatg gacaagcggt aaatgatata
     1741 ggtgaattgt ggaaaaaatt tatgggcaaa gggatatata atgctataaa gggtaaaaag
     1801 aatgacaaga caataggtct atatacagat taccagggtg attttacaag tccgtatagc
     1861 tttgttgcat gttgtgaagt aaatagtaat tctgataaag agaagaattt agaagaatta
     1921 aacactaata atactgttgg agaaagtatt attagtaaag ttataccagc aggtaagtat
     1981 gcaaaatttg ttatagtagg aggtcaaaaa gaagtaggag atttttggtt taagttttgg
     2041 caaatggatt ttgataggac ttatataagt gattttgaag agtatcaatg taatactttt
     2101 gatacaaaga aacaagaagt tcatatttac ataggaataa agtagatttg ttattactat
     2161 attgaatgaa gtattgagta tagtacattt attatagata gttttattat ttataactat
     2221 aagcagtaaa tctgtaaaag atttactgct tttttagtaa ttaatattat cagtatcact
     2281 ttgaatcttc agacatattc ttcatttaat attgtttatt gataaatgat acttgtaaat
     2341 ataatgattt gttatttagt ttaaaatatg aaaaaaagta ttataaacac attagaatac
     2401 ttctggattt atgggtatac aatttgaaaa atataaaaaa taatgtataa agctaaatct
     2461 aatttaatcg ataatacata tagagaaata taattaagta aataaacaac tatctattag
     2521 atattgagaa agtcaaatcc tatatgaatc tacagaaatg attttaatga atctgaaaat
     2581 tttttttata caaacataaa aaaggtataa agttgtttta ttataattta attacttttg
     2641 ataacttttt atgttttata caattatatt tttaataact cgtcaaaacg ccaaacatat
     2701 attacaatag atatataagt ttttttgttg acaaattatg ttaagagaat taagtataat
     2761 atggcataaa taattacttt tatttatcaa tgaaatgtat ttattgtgta gcgaaaaata
     2821 gtcagatagt atagacaata actgaagata aaagcgttta ttgttgtaca tatggaggag
     2881 ttagttttat ggaaaataaa attttgatag tagatgatgc tatttttatg agaatgataa
     2941 ttaaagaagc tctgagtaaa agtggctata ataatttaat agaagcaaat gatggagaag
     3001 aagcgtgtaa tatatttaaa tatgaaaagc ctgatttgat gttgcttgat attacaatgc
     3061 caaaaaaaga tggtttagag gtgctaagag aaataaaaaa gttggattca agtgctaagg
     3121 ttgtaatgtg ctctgcaata ggacaagaga atatgattgt agaagccata aaacttggag
     3181 cgttagattt tatagtgaag cccttcaaaa cagaaacatt agttaaagtt gtagataatg
     3241 ctttaaaata aaagtgtaaa tatacactga tacaatgaaa agcaaataaa tgcaataata
     3301 aagggagggg tttatattat aaattattta gaacttgatg atgttcatat agatgctctt
     3361 agagaaattg gtaatatagg ctctgggaat gcaattactg cacttgcatc catgataaat
     3421 agcaatgtag aagtattaat acctatggtc aagatacttg aatataatga agcgacaaat
     3481 ttgcttggtg gtccagaaaa caaagtagta tgtattttac tcgatatgaa aggtgatatc
     3541 aatggaatgt ttatgttttt gttggatgaa tcaataactc aacttatgct aagttctctt
     3601 tttaataaag aagaagcttt tttagatgag attgaggcaa ttgaaatttc agcaattaag
     3661 gaaataggaa atattatggc aagttcatat gtaaatgcta ttgcatctat gttaaacatg
     3721 actataagtg tttcaattcc agatatatgt attgatatgg ttggagctgt tctaaatgta
     3781 cctatgataa gattttcaga tgttggtgac aaagtcttat ttatagaaaa taaatttaaa
     3841 atgagtgata attattttac tagtcacatt ttgatgattc cagaaatgag ctcacttaaa
     3901 gagatactcg tgaggttggg attagaggta tgaatagaga gatagtagtg aacattgcag
     3961 atatgaagat agcctataga ccaaatgttt tagttactta tgcacttggt tcttgtgtag
     4021 gagtatgttt gatagataaa gtagctggaa ttggtggaat gttacacgtt atgttgccat
     4081 atagtaaaga tgctattaac attgaaaata agtgtaaatt tgctgacaca ggaataaatg
     4141 agctcattaa atctatggag aatataggtg caaatagaat atatataaaa gcaaaaattg
     4201 ctggaggtgc tcagatgttt ttaggaagta gcaattcagt aattgcaagt ataggacata
     4261 ggaatgttga ggcagtaaaa aaaactcttt taggattaaa tattccaata ttagctgagg
     4321 atacaggtat gaattatggt agaacaatac gtttttacac tgagacgggt gaattacttg
     4381 tagattcaat aaataaaggt aagaagaaat tataatatat atttcataga aagtgtattt
     4441 taggagggag gtattaaatg agatttccag atgacccata tttagattat ggactaattg
     4501 atgaaggaat taagtatcta aagtttaaag tagatgagca agattttggg gttgaacttg
     4561 aaaagataat agaaattact ggaaatcaag aaattatatt tattccagaa ctacctacat
     4621 attctaaagg cataataaat ctaagaggaa aaatcattcc gataatagat atgcgtttga
     4681 gacttaaaaa agatgaatta atgactacaa attgcatgta tatagtagta actgaaataa
     4741 aggacttggt agttggattt gtggttgata gagttgatga tatagtaagc ttagaaaaaa
     4801 gtaaaatatc acctccacca agagttacta tagaatattc ggattatttt gtaagtggag
     4861 ttggagaatt agaaggtaaa ataattactt tactagattt agagaaactt ttgacagata
     4921 aagatgaagt tttaatagat gaattaatca atgggtttga ttgcactatc atgtaatata
     4981 gggatgtagt tggagtgtta ttataaaaag attgtgatga gaacatttaa gaatatagtg
     5041 gtataattat taagtataaa aattttaaat cattaaaatt tgaatatatt atgtttaaga
     5101 gaggtttgta tatgaaaaat aagcaacagg gaacaatttt agtcgttgat gatagtgttc
     5161 tgatgtgtga cttaatagat aaaacactta caaaagataa ttttaaggtt aaaaaagcat
     5221 ttaatgcaat agaggcaaaa gaatttattg aagaatttat accagacatt attttgcttg
     5281 atataatttt accagataat tctggttttg agttttgtaa ggaagtaaaa tctaatgctc
     5341 gtacagcaga tatacctata atttttataa cttcaaaaaa tacagataca gacatagtaa
     5401 aaggatttgg tgtaggagca attgactata tggtgaaacc ttttagtatg acagaattaa
     5461 aagctcgtgt tatagctcat cttgaagtaa agaaatctca agataaacta aaaaagataa
     5521 ataatgaact agaatcttct cttgaaaaat tgaatagact cgttgtaaga gattatctta
     5581 caggtcttta caataggcga catataatta atcagcttat ggagcaaaga agaattaata
     5641 aatatagtga aacatcaata tcccttatcc ttggtgatat tgatgacttt aaggttatca
     5701 atgatactta tgggcatgaa gcaggggatt tcgtgttaac agtaatatca agtataataa
     5761 aaaagaattg tagacaaatt gatactgtat caagatgggg tggagaagag tttttgataa
     5821 tgctcacaaa tactccaata gaaggtgcta agatactttc agaaagaatc cgtaaagaga
     5881 taaaggagtt tgatttcaac tataaagaac ataaaataaa gtgcactatt acattaggag
     5941 tagtagaagt aaactcaaat atatcattag aagaaaacat atccttagca gataaagcaa
     6001 tgtatgaggg taaaaactta ggaaaagatt gtagcgtagt gagtacaatg aaaggattaa
     6061 aaaagatata aacttacttt atgaaggagt acagatatga aaagagaagt aaagctttct
     6121 aaattagcaa ttgtattgct gattattata gtattggctg ttctagcagg gattttttct
     6181 tctatttcat cttataaagc atttgaaaaa gtagaaaaaa gtattaatga tatgaataat
     6241 ttgaggactc aaagtcagta tataaaagat acaactagag agataagtga tgtattaaga
     6301 agatatgtat ttacaggaga aaaaaagaat aaagatattt atgaaaaaga attatacgga
     6361 aaaacaagag aatcagcttt aaacaaatta gaaaaaattg gtcttacaaa gagcgagtca
     6421 aataaaatct tagaagcaaa agaaatatca gattctttag taccaacaga acaaagtgca
     6481 ataaaggcag tcgaaagtaa tgataagagt aaagctcaaa gtataatttt tggtagtgac
     6541 tatataaact cccaagagaa aatggattct ttaataaatg aatttcaaga ggatatagtt
     6601 acccgttcaa attccaaaat tgtagaagta aaaaaagaat tacaaagtct aatattaaga
     6661 acaatattta tactttccct tttaataata ttgacaataa tagaatatac agtaataaaa
     6721 ttaaaaatag tgattcctat taataaagta gaaaaacact tttcaagaat tgcatctggg
     6781 gacttaagaa caagtataga cgttgaagaa agtaaatctg aaataggact attagtgagt
     6841 tctgtcaaaa agatgcaaag tatgctttta aattacatag accttataga cagtacatta
     6901 actagtattg caaaaggaga tttaagagta gagatagacc aagaatttat aggtgatttt
     6961 aaatcaatta aaatatcttt agaaagtata ataaactctt ttaatggtga tttttctgaa
     7021 ataaatttat cagcagagca agttgcaagt gcttcagaac aagttgcagc aggtgcacaa
     7081 gctctttcac agggtgctac tgaacaagct agttcagtag aagaattagc ttccacgatt
     7141 aatgagatat cagatggaat taagaagtgt gcagatagtg ctagtcttgc atgtgaggta
     7201 tctgaccaat ctgcatcaca agttggtttc ggaagtagat gtatggatga tatgatgaca
     7261 tcaatgggtg aaataagtga aaaatcgagt gaaatatcaa aaataatcaa aacaatagat
     7321 gagatagcat tccaaactaa tattcttgca ttaaatgcag ctgttgaagc agcaagagca
     7381 ggtcaatatg gtagaggatt tgcagttgta gcagatgaag ttaggtcttt ggctggtaaa
     7441 agtgcaaaag cagctcaaaa tacagctatt ctcatagaag aagctattaa aacagtagaa
     7501 aatggtgtca gaattgctaa agagactgca aatgcattgg acttggtagt aaatggtgta
     7561 gaaaaaacta ctaattatat taatggtatt tcttcggctg tacatagtca agaagtagca
     7621 attacacaga ttttatctgg aatagagcaa atttccatag tagtccaaat gaactcagca
     7681 acagcagagg aaagtgcagc agcttcagaa gagatgtctg ctcaagctca aattttaaaa
     7741 gaattagtag aaaaatttca tctaaaggaa agtgaagtaa gagaatcatc atgggtttaa
     7801 attatgaggt gaatattgaa gtaagagaat tatcgtgtat ttaaattatg agatgtatat
     7861 atattgaagt aagagaataa ttgtgtattt aaattatgag gtacatatag tgagataaga
     7921 gaatcatcaa ggatttaaat tatgagatgc atatataata gaaaattatt tgtaggtcgt
     7981 aggtgataaa tgtgaattat attgaaccga gtatgagaca aatgttagat atgtttatat
     8041 ttgagacaag tttattactc gaacaattag atgaaattat gattcaaata gaaaaagaga
     8101 aagtatttac tcataacaac attaatgaaa cttttagaat aatgcatacg ataaaaggtt
     8161 cagcatctat gatggggtta gacaatatat ctgctttagc acatagtgta gaagacttgt
     8221 tttatgctat acgtgaaggt aaaatagaag ttttagacaa tgacctatta tttgatttag
     8281 tctttcaatc attagatttt ataagaagag atatagatga gttacaatca agaggttgta
     8341 tatcttctga ttactctaat tttattgaag gaattgaaga gtatgtgagt actttggaat
     8401 tatcaagttc tatgaaatca aaggataatg caaatttaga aagtaaagac gaaaaagaag
     8461 taatcgattc aaattttgaa tacaaagaag actctgatgt agtaaaaata atctttgaac
     8521 aaggttgcat gatggaaaat gtgagagctt tcatgcttgt atttgaactt agagattact
     8581 gtgaatttat agatttttat ccaaaagatg tagaaacaaa tgcagagtct acagagtata
     8641 ttaaaagtta tgggttttat atacaatttg tagctaaaga ggataaggca atagtttata
     8701 aaactataga agataatctc tatgtaaaag aatacataaa tattagtaaa aaagaaatag
     8761 aaatgaaaag tacatcaaac aatgatgcag tagaaaataa attaaaagat aataataaag
     8821 taagtaaaag ttatcaaaat aactatacag aagatgagat acaagaagat gttttaaatg
     8881 aaaaaactga tatggaaaaa gatagtctaa gtgaagaaaa tgaacattta ccaataagta
     8941 agcagagtat tatgagtgta aatttagaaa aattggactt acttcaaaat atagttggag
     9001 aaattgtaat tatggaatca atggttgtaa atagcgtaaa ttctacaggt gttgatatag
     9061 ataatttcaa taaggcaaca agacaattac acaagcttac agatgaattg caagatatag
     9121 taatgtctat gcgtatgatt caaattgctg gtgtatttca aaaaatgcat agaattgtac
     9181 gagatatgaa taaaaaatta gataaaaatg tagagttaat aactatagga gcagagacag
     9241 aggtagataa gagtgtaatt gataatttag cagaaccttt tatgcacctt ataagaaatg
     9301 ctatggacca tgcaatagag cctattgagg agcgtatagc gaaaggtaaa gaagaagtgg
     9361 gaaagatagt tttatctgct gaaaatctag gcggagaagc tgtgataagt atttcagatg
     9421 atggtagagg cattatgaga gataagattt tggcaaaagc taaggaacaa ggtcttctta
     9481 aacatcaaag taatgagtat actgatgacg aggtcaattc tttgattatg actccaggct
     9541 tttctacaaa agaatctata actgaatttt ctggaagagg agtaggcatg gacattgttc
     9601 gtaaaagtat tgaaaaagtt ggaggttcaa ttgaagtaaa aagtaaacag gatgaaggga
     9661 caacctttac tataaaaata ccattaactc tttctatagt agagggtatg aagtttaaag
     9721 ttggagattc actatttact ttaccaatat catctataag aagaacattt aaacttacag
     9781 acttaaatca aataattaca gaaggaaata agacagagtt aattacaata tatggagcct
     9841 gtattcccat tataaggtta cataaaatat ataatattca aacaaaagta accaatctga
     9901 tgaatgggaa tatgataatt attgaaaatg agcataaagc agtatgcatt tttgttgatg
     9961 aaataatagg taatcagcaa gttgttgtga aaccatttcc agtttatttt aaccgattta
    10021 atataaaaaa taagggttta tcgggatgta caatacttgg ggatggaagt ataagtttaa
    10081 ttatagatgc agatagcatt atagaaatat attaggggat ttaaagtatg gctaaagatg
    10141 aaattattaa acaagtgtta acattttatg tcaatgatgt tatttatgga atagaattag
    10201 aaaatgtgat tgaaaccata agatttcaat caataacgta tgtaccatgt cttccaacat
    10261 atataagtgg agttattaat ttgagaggaa gaataatacc agttattaat atgcatataa
    10321 agtacaatct tcctaaagct gattataatg agagaacttg tataataatt acaaaggttg
    10381 atgagtatca agttggaatt attgtagata aagttgttga tgttattcat atagaaaatc
    10441 taaatttact agagacgact aattctaaca acactaatat taataaagat ataaaagaga
    10501 ttgcgaaagt tgaagataat tctatattaa ttttagatat tcgaaagttt ttgattaata
    10561 gtatatagtt agttctgata aattagcagg aggaagtttt tgcatgataa aactcacaga
    10621 tgaagaattt atagttcttg taaaccatgt aaaaaaagaa tatggtattg atttaagtaa
    10681 aaaaagagcg ttaattgaag gtcgcttata caatacaatg atagagaaaa aacttagttc
    10741 ttttagtcaa tatatgaatc tattatttaa agataagaca ggaaatgaag ctataaacct
    10801 aataaataga ttgagtacta accacacatt ttttatgaga gaacctcaac attttgagtt
    10861 tatacagaat agtatacttc ctttttggga agaaaataat aaaataagaa acataaatat
    10921 atggagtgca ggatgttcta gtggagaaga agcgtattca atagctatga ctttggatga
    10981 ttattttgga tataataaag agttgtggaa cattaaaatc atagccacag atatttcaac
    11041 taactcactt gaaaaagcta aaagaggtat atacatagaa tcaaatgtta acaatgttcc
    11101 tgagttatgg aagaagaagt attttataga caacaaggat ggtacattta aaatttgtga
    11161 taagattaga aaaagtgtaa tttttaagcc gtttaattta atgaatgatt tctcatatca
    11221 acattttgat ttgatttttt gtagaaatgt tatgatttat tttgatttaa aaacaagaga
    11281 agaacttata aataaatttt ataatgctac caaaaaagat agttttttgt ttgtaggaca
    11341 tgcagaagta atcaacagaa gtagtactaa atacaaatat gtaaaacctg ctatatataa
    11401 acgataaaaa ggtatttttg atatatgatt ttttggaggc ggtaatgtgg aacgttttaa
    11461 taatatggtt attgctatag gcgcatctgt aggtggtaca gaagcaatac ttgaaattat
    11521 aaaagaccta ccaaaatcaa caccagggat tgttgtagtt caacatatgc cagctatatt
    11581 tacttatatg tatgcacaac ggcttgacaa gcagtgtatt atgaatgtaa gagaagctaa
    11641 gaataatgat agagtagagc aaggaaatgt gttaattgca cctgggggtt accagatgaa
    11701 attgtgtacg gataaacaag gatattatgt aacttgtgaa aagggagaaa gaataagtgg
    11761 tcactgtcca tcagtagacg ttttgtttga ttcagtagca gaagtagcag gtaaaaattc
    11821 aataggaata atacttacag gtatgggtta tgatggagca aatggacttt taaaaatgaa
    11881 agaaaatggt tcatttacaa ttggacagga tgaaaattct tgtatagtat atggaatgcc
    11941 aatggtagct tttgatagag gttctgttat gttacaatta cctcttggtg aaatttcaaa
    12001 ctgtttgata agacatatta atattttaaa acaaaaagat taatttttta taaataaaaa
    12061 ttaatctttt attaaattat atggataaaa aaccctagaa actccaaata taataagaga
    12121 gatttatatt taggaggaaa atgataatat gaaatttttt aaagtcgata caaaagctaa
    12181 agttttaaga tatgtatttg ttttaacatt gactataatg gttgttttgg caattaaaca
    12241 tgactataaa tataataatt taaggaaaga tgcttcaatt aatttaaata gtaataagga
    12301 agcagtgact gttatgagta accagtcatt accaaagggt aatacaaata gtaaacaaac
    12361 tttatcagta agtacaatag gaagtaaaac aatagtgaaa aatgatgagt atttagaaag
    12421 tactataaac atgcctgtaa taacaaattc aaataaaatt gttgaaaggt ctacaaatga
    12481 taagataaaa aatgacatat ttgaatttta taataaatca tataaagaag caaagcagta
    12541 tcttaaggat aatcctgacg agaaaaataa gtttgttgca aatgtagact ttgaacttaa
    12601 aaagaatact gattctgctc ttagtataaa agtgagatat tatacttata gtggtggagc
    12661 acatggattt tatcaagata tagcatataa tgtggatatg agaactggaa aatttttaga
    12721 attaatggac ttgtttaagg ataatactaa atataaagaa gttatagatg aagaaattaa
    12781 aagacaaata gctgaacttg aaaaaaagga tgaagaaaat ataggaatat ataattttaa
    12841 aggtataaaa gagaatcaaa acttttactt gcaagatgag aatctagttg tatattttga
    12901 tttatatgat ataactccat atgcagcagg gataccagaa ttttcaataa ataaagcgtt
    12961 gataagttct atgctaaaac cagagtatgt agatttattt gatttgaaat agaagattaa
    13021 attaaatgtt atgctataat atttagcata gagacttatt aaaattgggg gtataaaaat
    13081 gctactttta aaaaaagacg acataaaaaa agtatttaca atgagagatg caattgaagc
    13141 tgataaggaa gcttttagaa tttattgtga aggaaagagt gtaaatcctt tgagaaccaa
    13201 tatatctgtt ccaagtcaag atgctagtat gttatttatg ccaggatatg tagaagaatt
    13261 aggctgtggg ggaataaaaa ttgtatctgt atttcctaaa aatgctcaaa aaggcaaacc
    13321 agtaattcca gcaacagtac tattattaga tggagaaact ggagaagtta gtgcagtatt
    13381 ggatggtact tatgtaactc agattcgtac tggagcagcc tctggagcag ctatagatgt
    13441 attggctaaa aaagattcta agattggagc tttgataggt ctaggaggac aaggtgagcc
    13501 acaacttgaa gccatgatag aagctagaaa tttggatgag gtaagagttt ttagtagaaa
    13561 caaggaaagt agagaaaaat ttgcagaaga aatgaactta aaattaagta aatataatac
    13621 taaaatagtt gcagttgaat catctgatga agccatagat aacgcagatg tgatagtttt
    13681 agctactcca tcaaaacaac cagtacttaa tggaaactta gtgaaaaaag gagctttaat
    13741 aagtgcagta ggttcatata tgccagatat gcaagagttg tgcccaaact gtttaactag
    13801 agcgtctaag atattttttg aatcaacaga tgcagtttta tctgagtcag gagatatatt
    13861 aattccatta aaagaaggta agataagtaa agatgatttt agtggtgatt taggaaatgt
    13921 tataaacgga actattcctg gaagagaaga tgatgatgag ataattgtat ttaaaactgt
    13981 tgggataggt gtacaagatg tcgtaacagc aaaaagaata tacgataaag caaaagaaaa
    14041 cggtattgga atagactgga agtggtaaaa tattcaaaat aattctatag ttagacaaat
    14101 ttatgttata atgttgataa ataaattttt tattatttta attgagggag gatgtttgta
    14161 tgaaaatggt aaaaaagtta atattatcta cgattatggc tctagtttta gttctaacta
    14221 tagggtgttc taatgcagaa gaaaaagtaa agtcgaattt tgaagctaat ttagctgcat
    14281 tagaaatgta taagtatgat gaaaaaggaa tttctgatgc attaacagaa gctgaatata
    14341 aacagattca aacttataac agcaaggaca aggtttcaat gattgctcaa ttgacacctc
    14401 tttatggtaa agaaaatgct gaaaagatat acgaaacaat tgttaaatta attcaaggtg
    14461 ttgaaaagac agtaacagtg gaatcttcta gtaaggataa gataactttg aaagttgtaa
    14521 gtaaagcttt agatactagt aaagtaatga gtgaagcgaa cactttagta aaatcttatg
    14581 taactgatga aaagaattac gataaaaaag aaaaagaagt tcaagatgat atgacaaaaa
    14641 tgattataga cctttacaat aagaaaccaa ctaaggaagt tacaactatg attacatata
    14701 ctaaaaaaga tggtaaatgg caaccaccta gtaatataca aaagttatgt gaaggaatat
    14761 atacaattta atttatttta aagacacaaa actatctttt aagcatataa taaaatgttt
    14821 ggaagata
//
LOCUS       DAEORZ010000104        14759 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEORZ010000104 DAEORZ010000000
VERSION     DAEORZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14759
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.104"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            92..1597
                     /gene="ade"
                     /locus_tag="KRH39_002732"
     CDS             92..1597
                     /gene="ade"
                     /locus_tag="KRH39_002732"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBG8512621.1"
                     /translation="MSPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADPHELVNV
                     KGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNNPRILGL
                     GEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDHECITFD
                     EVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDIEKQGHI
                     RWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLDKMEVDS
                     VYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEIVPYQIL
                     TNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIATTVAHD
                     SHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFTAEEVQE
                     ITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            1949..2413
                     /gene="bcp"
                     /locus_tag="KRH39_002733"
     CDS             1949..2413
                     /gene="bcp"
                     /locus_tag="KRH39_002733"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBG8512622.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            2423..3166
                     /gene="yaaA"
                     /locus_tag="KRH39_002734"
     CDS             2423..3166
                     /gene="yaaA"
                     /locus_tag="KRH39_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBG8512623.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            3294..5951
                     /locus_tag="KRH39_002735"
     CDS             3294..5951
                     /locus_tag="KRH39_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG8512624.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      6005..6107
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            6217..7482
                     /locus_tag="KRH39_002736"
     CDS             6217..7482
                     /locus_tag="KRH39_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBG8512625.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            7512..8402
                     /gene="metA"
                     /locus_tag="KRH39_002737"
     CDS             7512..8402
                     /gene="metA"
                     /locus_tag="KRH39_002737"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBG8512626.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            8893..9336
                     /locus_tag="KRH39_002738"
     CDS             8893..9336
                     /locus_tag="KRH39_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8512627.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            9367..11184
                     /gene="ftsH"
                     /locus_tag="KRH39_002739"
     CDS             9367..11184
                     /gene="ftsH"
                     /locus_tag="KRH39_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG8512628.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            11362..14064
                     /locus_tag="KRH39_002740"
     CDS             11362..14064
                     /locus_tag="KRH39_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBG8512629.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGTGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            14098..>14759
                     /locus_tag="KRH39_002741"
     CDS             14098..>14759
                     /locus_tag="KRH39_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8512630.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IIT"
BASE COUNT         5778 a         1693 c         2712 g         4576 t
ORIGIN      
        1 gaattagtaa gacatatttt agtatgcaaa attctatgaa gcaggaaccc tgtgacttta
       61 gtcatgggag gttcagtgta gcaataaata tatatcacct ggctttattg atgcacatat
      121 gcatattgaa tctacaatgg taatgccaat tgaattatcg aaaaaattat taaaatcagg
      181 tacgacaact atcatagccg acccacatga gttggtcaat gtaaaaggag ttagtgcaat
      241 agatttttta ttagaatcga caaaagatat accacttaat gtatatatta tggtgccttc
      301 atccgtacct gcaacaagtt ttgaaactaa tggagttggt aaatttagtg caaaagacat
      361 ggagagttat gtaaataatc ctagaatttt aggtttagga gaggtaatgt gttttaatga
      421 tgttataaat tctgaaaatg aaatcttgga taaactagag ctttttaaaa ataaagttgt
      481 agatggacat gcacctaata taaatggtaa atcacttcag acatatgtat gtgcaggaat
      541 agaaaatgac catgaatgta taacttttga tgaagtatat gaaaaattga gagcaggatt
      601 aaaaatttta ataagagaag gaagcgcagc aaaaaattta aagtctatag taagtggtat
      661 gctaaagcat aacttgccaa ttgaagaatt tatgttttgt acagatgata agcacttaga
      721 tgatatagaa aaacaaggtc acataagatg gaatataaaa tgtgcgattg atttaggtat
      781 ggaacctgtt agggctataa aagttgcaac ttataattct gcaaaagcat atgggttaag
      841 gaaaattgga gcaataggtg cgggttataa ggcagatatt gttgtattga atgatttaga
      901 caaaatggaa gtggatagtg tatataaaga tggtaatttg gtaaacgaag agatgttttc
      961 taattacaat tatgagatta aagataaaga attgcttaat acagtcaagt ttaaatatat
     1021 taataaagaa aaaatacagt taaaagtaag tgaaaaaaat tatgtaatgg agattgtacc
     1081 ttatcaaatt ttgacaaata aggtatatga atctttacca tgtgcagatg gatattttgt
     1141 tccaaataaa gagtattcaa aactttgtgt tgttgagaga catagaatga ctggaaatgt
     1201 agcaattgct ccactaaaag gatttggcat aaaaaatgga gcaattgcta ctactgtggc
     1261 ccatgattct cataatatta tagttgcagg ggataatgat gatgatattc tagtagctat
     1321 aaattactta aaggaaatac aaggtggtta tgtaattgta tcaaatggaa aagttcttgc
     1381 acacctaagt cttcaagtag ctggattaat tagtactttt acagctgaag aagtacaaga
     1441 aattactgat aatatgttgg agatagcaag aaaaatggaa gttccagagt atgtagaccc
     1501 ttttattaca ctttctttta tggcacttcc agttgttcca caaataagac ttacagattt
     1561 aggtctattt gatgttgagg aatttaaatt tatatagata gtataaaagt aataataaat
     1621 gaggctttct caaaatagag ataagtctct ttttattgtt gaaatgatga aaatatattt
     1681 gatttcatac aaaaaagagc atctcatgaa ctaaaaattt tatttttaga tatccttatt
     1741 ttaattaatc tatcatttaa cattgtttta agcatagata tcttaataaa actattttca
     1801 gtctatttta acatataaat acatgaaaat tatatattta tgaaacttta tacataaaaa
     1861 ataaaatatt ttatttatat atagatttat agggtaataa tataagcata gtataaaatt
     1921 attaataaaa acaaaaggag agatatttat gttgagtata ggaacaaaag caccagaatt
     1981 tacactagaa gataaagatg gaaataaggt aagtatgtca gattttaaag gcaaaaaagt
     2041 agtagtatat ttttatccta aagataatac accaggatgt acacgtcaag cttgtgcttt
     2101 tagaaatgca tatgatggat ttaaaaagga agatatacaa gttattggga taagtaaaga
     2161 tagtataaaa tcacatcaaa aatttgcaga aaagcatgaa cttcctttta ttttactttc
     2221 tgaccctgac ttagttgcta taaaagcttt tgatgtatgg aaagagaaaa aaatgtatgg
     2281 aaaaactgca ttgggagttg tacgtgcgac atatattata gatgaaaatg gaattattga
     2341 aaaagtcttt gaaaaagcaa aaccagacac aaatgcacaa gagattcttg aatatttgga
     2401 aaaacaggag taataagaat ttatgattat aatgttatca ccagctaaaa atatgaagaa
     2461 tatagaagta tttgatagag atttgagttt gccttgcttt atagataaca caaaagaaat
     2521 agtggaaaat ataaagacat ttggtataga agattttaaa aataaaatga aaattaatga
     2581 aaaattagct gtattaaata aaaatagatt tgaaagcatt aaatttgata gattaggaaa
     2641 ccctgcaatc ttaacttatg atggaataca atataaaaat atagaagcag aaaattttac
     2701 aaggaaagat gaagagtttg caaatagctg tattcgaatc ataagtggat tgtatggtgt
     2761 tgtaaaacca tatgatagta tatatgaata tagattagaa atgcagacaa aattacaagt
     2821 aggtgagttt aagaatttat acgaatattg gggaaatcgt atatataaag aactaatcaa
     2881 agaaaaaaca gctatcgtta atctttcatc aaatgaatac agcaaatcca tagaaaaatt
     2941 tattaaagat agtgatacat atattacatg tacttttaaa gtaaataaaa atggcatatt
     3001 gaaagtggaa tctacacaag caaagaaagc tagaggtatg atgacaaaat acattgtaaa
     3061 aaataggata agagatattg aagaactgaa aaagtttgat ttagaaggtt ataagtataa
     3121 agaaaactta tctaacaata gtgaatacat atttgtaaaa gagtagaagt tttgttaaat
     3181 attaaatggc attgttaaat ttaggtaaaa tatacttagt gtgtattgaa ttgaacttga
     3241 ttttaaaata taattattct taaattaaaa aaagaaagga gtggtcttcc aaaatggaaa
     3301 atcacaaaca gatgccttgg cattcaagag caacgcatga aattttagaa agtctagatt
     3361 catcagagga ggggctcacc gataccgagg cggaaaaacg gcttaaagag aatggcttaa
     3421 atgaacttag aaaaaaacca cctagaacaa ctttacaaat gctttggagt caaattacag
     3481 atgctatggt tatgatacta ataggagcag caatattatc tttgatattt ggcgaattta
     3541 cagaagcttt tgtaatacta ataattgtaa cagtaaatgc agttattgga attgttcaag
     3601 agaaaaaagc agaatcatca ttagaagcat taaaaaatat gaattcacct acagctcgtg
     3661 ttatgcgtga tggtgaagaa aatgttattc cagcaagtaa tcttgtagta ggagatattg
     3721 tttttttaga agatggagct atggttccag cagaccttcg tttaatagaa acatcaagtt
     3781 taaaaattca agaggcttct ttaacaggag aatctgttcc atcagaaaaa gatgcagata
     3841 caatcttacc taaagaatgt gtattaggag accgtagcaa tatggcgtat acatcttcaa
     3901 tagtcacata tggtagaggt gtaggtgttg tagtagcaac tggtatggat acagaagtgg
     3961 gtaacatagc acatctttta gattcacaag atgattttga tactcctata aaacgtaaac
     4021 ttaatactgt tggaaagaca ttaagtgtca taggtattat tgtatgcgtt gttatatttg
     4081 caataggtgc attttatcag agacctctct tgccaatgtt tatgattgca atttcccttg
     4141 ccatatcaat tattccagag ggattacctg caacagctac tattgtaatg gctttaggcg
     4201 tacagcgtat ggctaaaaga aatgctttaa ttagaaagtt acctgctgtt gaaacattag
     4261 gaggagctac agtaatatgt tgtgataaaa ctggtacgtt aaccttaaat aaaatgactg
     4321 ttacacatat tgctgtcaat ggagattttg aatctggaaa agtgacatca atagatactg
     4381 catctgaaaa acatccatta gtgtataaag agattgttta tgctggtgca ctttgtaatg
     4441 atgcatgtat taatccagat aaaacagatg aaatattagg tgacccaaca gagggcgcat
     4501 taatttttat ggccaaaaaa tttggaatag atcaagaagc tcttgaagaa gaatatccac
     4561 gtgtatttga acagcctttt gattcagata gaaaatgtat gacaactgtt cataatatag
     4621 atgaaaagat tattgcttat actaaaggag cagtagacga aattcttgac ctatgtacta
     4681 aaatattaac ttcaaaaggt gaaagaaaca ttactgaagt ggataaaaag aatatacacg
     4741 aattatgtct taacatgtct aaagatgcat tgcgtgtact tggttttgct aagcgtgaga
     4801 tttcatctat accaaaagaa gatagtgaaa atatagaata tgatttgaca tttataggta
     4861 tggttggtat gattgaccca ccaagaactg aggtaattga tgcagtaagt acttgtaaag
     4921 aagcaggtat aagaaccata atgataacag gtgaccataa agttactgcc acaacaatcg
     4981 ctcatgagtt aggaatatgg agtgaagaaa atactgtgat atctggtgat gaacttgata
     5041 atttgagtga tgatgaacta gatgaagctg ttaaaaatac tacagtattt gctcgtgtat
     5101 ccccatttga taaacttaga attatacaat ctctaaaaag aattggagaa gtaccagcaa
     5161 tgactgggga tggtgtaaat gactctcctg ccttaaagtc tgctgatatt ggtatagcca
     5221 tgggtataag tggtacagat gttgcaaaag attcttctga catgatttta atggacgata
     5281 gttttacaac aattgcttat gcaataaaag aaggtagacg tgtatataga aacatacaaa
     5341 aagttataca atttttgctt gttggtaata tagcagagat acttacgttg tttgtagcaa
     5401 tattattaaa ttgggatact ccgcttcttg ctgttcatat tctatgggta aaccttgcaa
     5461 ctgctacgct tccagccctt gctcttggag ttgacccacc aagcagaaat ataatgaagc
     5521 accagccagt gaaatcaacc actttgtttg aaaaaaatct tatacaaaga gttgttaccc
     5581 aaggtatatt tgtggcaata atgactattt tagcatataa aattggtctt aatatagaaa
     5641 atcattctgt aggtcaaaca atggcatttt gtgtattagc attttcacag atgttacgag
     5701 catttagtca gcgttcaaat acagattcaa tgtttaatag aaataatggt aaaaatccat
     5761 ttcttttaat ctcattttta acttctgcat ttttaatagc agtaatactg tttgtacctg
     5821 tttttagaaa tgcttttaac ttaacagttt taaataactt agagtggctt ataacgattg
     5881 ttttagccat aatgtctgta attcaagtag aggttgggaa aattattaaa agaaaaaaat
     5941 taaaaaaata gttgacaaaa ttaggaaaaa tatataagat ataaaacata gaaaaattaa
     6001 ataaatctta tccagagagg tggagggact ggccctatga aacccggcaa ccagtatatt
     6061 tatatacatt ggtgctaaat cctgcggcga aagctgagag atgagtataa ttaagcttat
     6121 acagagcctg agttatatac tcaggcttta tttattttaa ctctcatctg gtaaatagtt
     6181 taattttttt tactaaaatc taagggggat acaagaatgt ataataaaga aacaatatgt
     6241 gtgcaaggaa attataaacc aggtaatgga gaaccaagag tactaccttt atatcaaagt
     6301 acaactttta aatatagcag tatagaccaa cttgctgaat tatttgattt aaaagttgat
     6361 ggacatatat attcaagaat aagcaatcct actattcaag cttttgaaga aaaaataagt
     6421 ttactagagg gtggagtatc ttctgtagct gtatcatcag ggcaatctgc aaatatgttg
     6481 gcagttttaa atatatgtaa atcaggagat agtatacttt gttcttcaaa agtatatgga
     6541 ggaacattca atttactagg acctagtctt aaaaaatttg gtatagattt aatatcgttt
     6601 gacttagatt caagtgaaga tgagatagta gaacttgcaa aggaaaatac taaggttgtg
     6661 tttgcagaaa cacttgcaaa tccaactctt gaagtcatag attttgaaaa aatagcaaat
     6721 gtagctaaga gaattaatgt tccatttatt gttgataatt cattagcatc tccagtgctt
     6781 tgtaaccctt taaagtatgg agcaaatata gttactcatt ctaccacaaa atatttagat
     6841 gggcatgctt caagtgttgg aggaattata gtggatggtg gaaactttaa ctgggataat
     6901 ggaaaatttc cagaattagt tgagccagac ccaacatatc atggtataag ctatactcaa
     6961 aaatttggaa atgccgcata tgcaactaaa gcaagagttc agttgcttag agactatgga
     7021 aattgtttaa gcccattcaa tgcgtatctt actaatttaa atgttgaaac actacatctt
     7081 agaatggaga gacatagtga aaatgcactt aaaatagcta gatttttaga aaaacatgaa
     7141 aatgtagatt ggattaatta cccaggactt gaagataaca agtattatga gaatgccaaa
     7201 aagtatttat caagaggatg tagtggtgtt ttatcatttg gagtaagagg tgggttagaa
     7261 aatgccaaaa aatttgtgga aaaattacag atagcatctt tggttacaca tgtttcagat
     7321 gtaagaactt gtgttataca tccagcttca actactcata gacaattaac agaagaacaa
     7381 ttaattgcat ctggagtatt gccttcacta ataagattat ctgttggaat agaaaatgta
     7441 gaggatttaa tagctgattt aaatcaagct ttaaatttct aatattctaa gagaaagaga
     7501 agtgaaaaga tatggcttta atactaccaa agggattacc tgttattaat aaattattag
     7561 atgaaggaat agatgtaatt tataaagaag attttaaaaa ggaattaaag tatgaagaaa
     7621 acatagacac taagatagct atactaaatc taatgcctat aaagatagat acagaattag
     7681 atttactaag aaggatagat aaaacaggat tcaacgtgtc tgtggaattt attaaaattt
     7741 caacaagaga aagtaaaaga agttgtaatg aatatgttaa agaattttat aaaacatttg
     7801 atgaagccaa aggagagtat tttgatggct ttataataac aggagctcca gttgaacaaa
     7861 tggagtttga agaagttgat tattggaatg aactagaaga aattatggat tattctaaga
     7921 gaaaaactaa atctacatta tatatatgtt gggcagctca agcaagtctt tacaaatact
     7981 acaatgtaaa aaaacttcca ctaagtcaaa aatgttttgg agtatttaag cataaggtag
     8041 ataaagatag taaaatagta gatggatttg aaaatgaatt ttttgctcca cattcaagac
     8101 atactactgt aaatattgaa gctttgaaag agaataaaga gttgagcatt gtgagtcatt
     8161 ctaaagaagc tggtccctat ataattacaa attcaagaga tgtgttcgta atgggtcata
     8221 gcgagtatga caaatacact cttgataaag aatataagag ggatataaat aaaggagata
     8281 aaatatctat acctcaaaat tactatatta atgatgaccc ttcagaagag ccaactgtaa
     8341 aatggaaaaa acattcagaa ttgttgttta gaaattggat aaaaaactat ttgatacaat
     8401 aaattttaag agtatataaa tgttaactaa aaaaatatag aaatttatag gtataaagtt
     8461 aatcttaatg atactttata ccttattttt ttaaaatttg accttataat agtagtttta
     8521 tattttaaga aaatcacttt aggtgtatgt agatagaggt ggctttaatt ttatatatgg
     8581 tgcgataatg ggagactaaa cctttatagt atcaataaat ttgtatttta acttttaaaa
     8641 gagattaaaa agggagaaat cagtgtaaaa tatatatata gattttttat taaattttat
     8701 taaaaatatc ctgtataaaa ttaaatatca ataagataac aaaatttcaa ttgaagttac
     8761 aatattgtaa ttgacaataa atacttaata ctataatatt taatacatta aacattatag
     8821 tgttaagtat ttatttacag tatgcacttc agcctaaatt tgaaataaag tattttcgta
     8881 gggagagttt gtatggatga gatgagtttt aagaaagagc tactagagtt gactagagat
     8941 ataaatatga aatttacaac tttattaagt gatttttatc agccacttgg tattacagca
     9001 gtgcaagctt tgattttatc tgagttatgt gaacatggag agaaaaaaat aagcgattta
     9061 gggaagaact taaacatgac aaatagtaat gtatcagtaa tctgtcaaag gcttgaaaaa
     9121 aatggctttt taaatcgcat aagagatata gaagaccaac gaattgtaaa agtaaaagtt
     9181 acaaataaat cattggatat acaagaacat atttcaagta gtatttttga tagttatttt
     9241 gaaaatatga ccagtgaaaa attgcaagat atggaagata taatagaagg tttagagaaa
     9301 ttaaataaac ttttaactta cattgattgc aaataacata aaaataattt aaaggagaat
     9361 aaaattatgg atgaacataa accccaaaaa aggtcagcac tctattatta tacagttata
     9421 ataatagtag taatgttttt aaatgcttta atatttccaa gtttaatgga taaaaaaatg
     9481 gttgagattg actatggaaa gtttctcaca atgttggata caggaaaggt taaatctgtt
     9541 gagatagaaa ataatagaat agcaataaat ccatctagta gcaaggataa gaatatatac
     9601 ataactggta gaatggatga cccagaattg gtagatagac taaaaaatgc aaaagtagaa
     9661 tttactaagg ttattcctaa ggaaaattca cctcttttaa gtattttact tacatggata
     9721 atacctatag gtattcttat gctatttggt aacttaatga tgaagtctat gcaaaagcga
     9781 atgggtggta atgctatgca atttggaaaa agcaacgcca aaatgtatgt atctgctcaa
     9841 agtggaaaaa gatttaatga tgtagctggt caagatgagg caaaagaagc tttaactgaa
     9901 atagtagatt tcttgcacaa tccagaaaag tataaaaaga taggagcaca aatgcctaaa
     9961 ggagcacttt tagtaggacc tccaggtaca ggaaaaacac tccttgcaaa agctgtagca
    10021 ggtgaagcaa atgtaccatt tttctcaata tctggttctg agtttgttga gatgtttgta
    10081 ggaatgggag cttctaggat tcgtgattta tttaaacaag caaaagaaaa agctccatgt
    10141 atagtgttta tagatgagat tgatactatt ggtaaaaagc gtgataatgg aagtggatta
    10201 ggtggaaatg atgaacgtga acaaacttta aatcagttgt taagtgagat ggatggtttt
    10261 gatggtggaa ttggagtagt aatacttgct gctacaaata gacctgacag tcttgacaaa
    10321 gcattacttc gtccaggaag atttgataga cgtataccag ttgaacttcc agaccttgga
    10381 ggacgtgaag ctatattaaa ggtacatgct caaaatgtaa atgtaagctc agatatagat
    10441 tataatgcaa ttgcaaggtc tacatcagga gcatcaggtg cagaacttgc aaatattgta
    10501 aacgaagcag cacttttagc agttaagtct ggcagagata tagtagaaca acaggatttt
    10561 gatgaatctg ttgaaactgt aattgctggg tatcaacgta agggagctgt aatttcagat
    10621 aaggaaaaga aaattatagc atatcatgaa ataggtcatg cacttgttgc tgctatgcaa
    10681 aaacattctg cacctgtcca taagattact ataataccta gaacttcagg agcattggga
    10741 tatactatgc aagttgctga agatgagagt gtacttatgt ctaaagaaga agcaattgat
    10801 aaaataacta cttttacagg tggtcgtgct gctgaagagg ttatctttaa cacttgtaca
    10861 tcaggagcat caaatgatat agaacaagct actaaaattg caagagctat ggttactcgc
    10921 cttggtatga gtgaagagtt tgatatggtc gctcttgaaa ctgtaagcaa tcaatattta
    10981 ggaggagata cttctcttgc ttgttcttcc gacactgctg ctaaaataga tgaagaggta
    11041 tttaaaatag taaaggcttg tcacaaaaat gcaattgaaa tcttagaaaa taatattgat
    11101 aaattgcatg aacttgctaa ttatttattg gaaaaagaaa ctattacagg tgatgagttt
    11161 atggaaatac ttaacttaaa ctaaaatgat tatacacaag ataaaaatgc ctattgttag
    11221 gcatttttat tttacaacta aattttttaa attcatattt aaatctttac ataatcttaa
    11281 tcattaaaaa tacatcttaa ctacaatttt atatcggaat tgatattata atagtaatga
    11341 atggtgtaag gaggtttttt aatggataga cctaatcctg atattctatt agaaaaaatt
    11401 aaaaatgaag aagaaaaact aagtagaggt caattaaaaa tattctttgg ttatgcagct
    11461 ggagtaggaa aaacctactc tatgttggaa tctgcacaaa atttaaaaaa agtgggtgtg
    11521 gatgtagtag ttggatatat tgaaccacac actagaccag aaacattggc tttacttgat
    11581 ggcttggaaa ttcttcctgt aaaggagata gaatataaat ctataaaact aaaagaattt
    11641 gatttagatg ctgctttagc aagaaaacca gaagttatat tagtagatga atttgcccat
    11701 tctaatgttg ctgggttaag gcatacaaaa agatggcaag atatagaaga attgctgtta
    11761 gcaggcataa atgtctacac tactgtaaat gtacaacatt tagaaagtct aaatgacatt
    11821 gttgagataa ttacaaacgt atcagttaaa gagactatac cagataagtt tttggataca
    11881 aatacacagt tagaacttat agatgttgaa cctgatgtac ttcttgaaag gttcaatgaa
    11941 ggtaagatat ataagaaaga acaagctact agagcaaaaa ataatttttt tataaaagat
    12001 aacttggttg cacttagaga aatcgcactt agaaaaacag cagagagagt taataaagaa
    12061 gttcaaatga ccagactatc aaagggtgat gtaactgtaa tacctacatc agatacactt
    12121 cttgcatgta taagtccatc tccatcttca gcaaaagtta ttagaactgc atccagaata
    12181 tcagacagta catttgcaaa atggatagct ttatatgttg aaactccaaa cacagcaaga
    12241 cttccatttg atgaacaaaa acaactacaa aataacctaa aattagctaa gaaattaggt
    12301 ggagaaataa ttgttttaca tggtgaaaat ataatagagc aaatacttag aattgcaaaa
    12361 ctgagaaatg taacaaaaat tgtaataggt agaaatcatt caagtaataa aagatttagt
    12421 aaaagattta aaaaagatat agttgacaaa ttaattgatg aagttgatta tatagatata
    12481 catataattc catataaatt tgcctcagat gttaaataca aacctaaaaa ggataaatca
    12541 tcaataaaat ctaaatttaa aattagcaaa gttgatttta taaaacttat atttattact
    12601 ttgatgataa gtattttagc atatgcagta caaagcatgg gatttataag agaaaatata
    12661 ttgttaatat atatgttggg tgtagtttta gtttctatgt ggactaaagg gtatagtaca
    12721 ggtataataa gttctgtatt taatatagtt cttttaaatt atttttttac agcaccatta
    12781 tatacattat ctatagcaga ttcaaactat atagtaacac tggttgtatt ctcgatagta
    12841 ggtataataa ctagcactct tacgtctaag atacagcatg aagcagaaac tgctgctaaa
    12901 agagaagaaa atacaaaaat gatatatcaa ataattaagg gatttttaag attatcaaat
    12961 aaagatgata tagtaaacaa aggtatagaa cttttgtctc ttagcttaag tagagatgta
    13021 atttgttatt taaatgtaga taagcataat aaatcaaagc tttacaaaaa aaatactaat
    13081 aataaggata aaaatgattt ggatagtgaa gatgaaaaag ctgttgcaag ttgggtttta
    13141 tccaattcaa cagtggcagg aaatgacacg gatacacttc ctggttcaaa gggatactat
    13201 ataccaattg taggaatgaa tttaacttta ggagtagtgg gaatatcatg tattgattca
    13261 aaattggata cagaagatat aagcttaatt gaaacaataa ttgctcaaat ggcaatagcg
    13321 cttgatagag aaatattgtc agaagctaaa gaaaatacca atcttgagat tgaaagggaa
    13381 agattaagaa gtaatctttt aagagcagtt tcgcatgacc ttagaactcc actagcagga
    13441 atatctggtg cagtaagtac aattataaaa aacaagggta ctataggtca agatataata
    13501 gatgaattac ttagtggtgt atatgaagat actcagtggt taataagact tgttgaaaat
    13561 ttacttagta tgaccaagat agatgaaggg aaattggaag ttaaaaaaca ttctgaactg
    13621 gttgaggaaa ttatatcaga agcgttacaa aaaataaaaa aaagaattga aaatgcactt
    13681 atagatatta atataccaga acagatatta tttgttccta tggatgcaaa attaatagaa
    13741 caagttttaa ttaatttaat agataattct ttgaaatatt caaaggaaga ttgtaagata
    13801 gagataaatg tatatgagaa agatgattat gtttggtttg aagtatctga taatggtact
    13861 ggtatatcaa aggaacttaa aaagcatatc tttgacagat tttttactgg agaagaagga
    13921 gctaaagatt ccagaaaggg tgttggactt ggattatcaa tttgtaaatc tataattcaa
    13981 gctcataaag gtgaaattat ggtagaaaac aataaagata agggttcaac ttttaagttt
    14041 agtttgccta aggaaaatga atagttttaa attttaaaat tagttttgga ggaagaaatg
    14101 agtaaaaaac ttatattgtt agtagaggat gataagacaa taagaaaatt tatatctact
    14161 gcacttttaa cacaggatta tgatgtcaag gaggctataa ctggaaaaga aggtatctct
    14221 attgctgtct catattctcc agatgttgtt cttttagatt taggcttaga ggatatggat
    14281 ggaatagagg taataaaagc tatcagacaa ttttcaaata taccaataat tgtagtttca
    14341 gctagagagc aagatagaga caaggtagaa gtatttgatg caggggcaga tgattactta
    14401 acaaaaccat tctccatagt agagttatta gcaagagtaa gagtagcatt tagacattca
    14461 caagtagaag ctcaacaaaa agatgatgtt aaatctacgt ttgaagtaga taaattattg
    14521 attgattttg ataaaagaaa agttattgtt gatgatgttg aagtccattt gacacctatt
    14581 gaatataata ttctttcatt acttgcaaag catcatggaa aagtgcttac tcataatttt
    14641 ataataaaag aaatatgggg tagtgttata gggaatgaga ctaagtcttt aagggtcttt
    14701 atggcaacgt taagacgaaa aatagaaaaa caacctgcaa acccaagata tataattac
//
LOCUS       DAEORZ010000105        14689 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEORZ010000105 DAEORZ010000000
VERSION     DAEORZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14689
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.106"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            106..390
                     /locus_tag="KRH39_002742"
     CDS             106..390
                     /locus_tag="KRH39_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBG8512631.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            423..2051
                     /gene="groL"
                     /locus_tag="KRH39_002743"
     CDS             423..2051
                     /gene="groL"
                     /locus_tag="KRH39_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBG8512632.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            2313..3053
                     /gene="larB"
                     /locus_tag="KRH39_002744"
     CDS             2313..3053
                     /gene="larB"
                     /locus_tag="KRH39_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBG8512633.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            3137..3871
                     /gene="larC"
                     /locus_tag="KRH39_002745"
     CDS             3137..3871
                     /gene="larC"
                     /locus_tag="KRH39_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBG8512634.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            3901..4320
                     /locus_tag="KRH39_002746"
     CDS             3901..4320
                     /locus_tag="KRH39_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBG8512635.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      4380..4481
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            4702..6237
                     /gene="guaA"
                     /locus_tag="KRH39_002747"
     CDS             4702..6237
                     /gene="guaA"
                     /locus_tag="KRH39_002747"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBG8512636.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGIPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            6776..8206
                     /locus_tag="KRH39_002748"
     CDS             6776..8206
                     /locus_tag="KRH39_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG8512637.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCSSKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(8354..9229)
                     /locus_tag="KRH39_002749"
     CDS             complement(8354..9229)
                     /locus_tag="KRH39_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8512638.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETALFDRLPRGVCLTRCGISLLEEARSILTACRNLDKRISHLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSEQALSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIKAAQAGLGITILPENLLLDSLLHKKLRLIELDGIDMENKMLAILHKDKNI
                     TQPLKIILDNISNVL"
     gene            9349..9912
                     /locus_tag="KRH39_002750"
     CDS             9349..9912
                     /locus_tag="KRH39_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG8512639.1"
                     /translation="MNQSRKTYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCIAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSAMIPIAFFANFVM
                     SINVIFILSVCCFVCVMQAFYKKRENK"
     gene            9909..10475
                     /locus_tag="KRH39_002751"
     CDS             9909..10475
                     /locus_tag="KRH39_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG8512640.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISATATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     TLLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(10604..10840)
                     /locus_tag="KRH39_002752"
     CDS             complement(10604..10840)
                     /locus_tag="KRH39_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512641.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(11150..14578)
                     /gene="uvrA"
                     /locus_tag="KRH39_002753"
     CDS             complement(11150..14578)
                     /gene="uvrA"
                     /locus_tag="KRH39_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG8512642.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPNTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESEITYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKTIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
BASE COUNT         5360 a         1830 c         2541 g         4958 t
ORIGIN      
        1 taaaaagtat atatataaat ctacatatat aagtagtatt aatttaaaaa taactaaaaa
       61 cataggcgaa ctagtctata cttaatattt taggaggcgt gaataatgaa aataagacca
      121 ttagctgaca gagtagtaat taaaaaagta gaagcagaag aaaaaactgc aagtggaata
      181 gttttaccag gagcagctaa agagcaacct caaatagctg aagttgtaga agttggacca
      241 ggtggaatag ttgaagggaa agaaataaaa atggaattga cagtaggaga taaggttata
      301 ttccaaaaat attctggaac agaagttaag atagaaggac aagaatacac aatactaaga
      361 cagagtgatg tattagctgt tattgaataa atatagaata aatttattag gaggggttta
      421 aaatggctaa agaaattaaa ttttcagagg aaacaagaag agctttagaa gctggtgtaa
      481 ataaattagc agatacagta aaagtaacat taggacctaa aggaagaaat gttatattag
      541 ataaaaaatt tggttctcca cttataacta atgatggagt aactatagca aaagagatag
      601 aactagaaga tagatttgaa aatatgggtg ctcaattagt taaagaagta gcaactaaaa
      661 ctaatgatgt agctggagat ggtactacaa ctgctacagt tttagcacaa gctataataa
      721 gagaaggttt aaagaatgta acagcagggg ctaacccaat acttttaaga aaaggaatac
      781 aaaaagcagt aacagtagca gtagaagaat taaaaaatca atcaagaata gtagaaacac
      841 aagaggctat atctcaagtt gcttctatat ctgctggtga tgaagaagtt ggaaaattaa
      901 tagcagaagc tatggaaata gtaggtaaag atggagttat aactgttgaa gagtctcaaa
      961 ctatgaatac tgaattagat gctgttgaag gtatgcagtt tgatagagga tttgtttctg
     1021 catatatggt tacagatgta gataaaatgg aagcagtttt aaacgaccca tatatattaa
     1081 ttactgataa aaaaatatct aacatacaag aattattacc agttcttgaa caaatagttc
     1141 aacaaggtaa aaagttattg ataatagctg aagatgtaga aggtgaagca ttatctacat
     1201 tagtagtaaa taaattaaga ggaacatttg atgtagtagc agttaaagct ccaggctttg
     1261 gagatagaag aaaagaaatg cttcaagata tagcaatact tacaggtgct caagtaatat
     1321 cagaagaatt aggttatgat ttaaaagaag ctgatttatc tatgctaggt agagcttcat
     1381 ctgttaaggt aactaaagaa agtactacaa tagtagatgg ttctggagat aaaaaagcta
     1441 tagaagatag agtaactcaa attaaacatc aagtagagca aactacttca gattttgata
     1501 gagagaaatt aatggaaaga ttagctaaac ttgctggagg agtagctgtt gtaaaagttg
     1561 gagctgctac agaagtagag ttaaaagaaa gaaagttaag aatagaagat gctcttaatg
     1621 caactagagc agctgtagaa gaaggaatag tagctggtgg aggaactgct tttgttagtg
     1681 ttataccagc aataggaaca ctaattgaga gtttagaagg agaagttaaa ctaggtgctc
     1741 aaatagttaa aaaagcgtta gaagagccat taagacaaat agctataaat gctggtcttg
     1801 aaggtgctgt aattgtgcaa aatgttgtta attctgaagc agaaactgga tttgacgcat
     1861 tgaatgaaaa atatgtaaat atgatagaag ctggtatagt tgacccaact aaagttagta
     1921 gaagtgcttt acaaaatgct gcatcaatag caagtacttt cttaactact gaagctgctg
     1981 ttgctgacct tcctgaaaaa gaagatgcag gaatgccagg aatgggtggc ggaatgcctg
     2041 gtatgatgta atttaaaaaa tttaaaatat aaattttaat ataaagaact atttcgactt
     2101 agattttaaa ctctaagttg aaatagttct tttgtatata tctaattttt gtatatctat
     2161 aaattcaata cacatattta gataatcagt atctagtttg taataatatt ttagagtagt
     2221 attgttattt tttaggttat gattttagat atatattaat taaggtataa ttgttgatag
     2281 agaataaatc aaatttaaaa ggatgcgata atatggactt aagaaaattg cttgaagaag
     2341 ttaaaaatga aaatattgat atagattttg cgttagaaaa gttaaaagat ttaccttatg
     2401 aagaccttgg atatgcaaat atagaccatc atagagaatt aagaaatggg tatccggaag
     2461 ttatttattg tgaaggtaag agtgatgagc atattttagg gattattgat aaaatgaatc
     2521 aaaaaagttc taacatattg gggacaaggt gcagaaaaga aacttttctg aaaattaaag
     2581 aaatctataa tcatgctgag tacgaagagg cgtctaaaat tcttaagatt caaaatcatg
     2641 atatagagaa tataggaaaa ggtaaaattg tgattgctac agggggaact tctgatatac
     2701 cagtagcaga cgaagcttat catacagcta agtttttagg taatgatgtt gatagaatat
     2761 atgatgttgg agtggcaggt atacataggc ttttaaataa aagacataaa atagatagtg
     2821 ctagagtaat agtagccgtt gcaggtatgg aaggtgctct tgcaagtgta attggtggac
     2881 tggttgatgt tccagtaata gctgttccaa cttctgttgg atatggagca aattttggag
     2941 gattagcagc attactagct atgttaaata gctgtgcatc tggaatatct gttgtaaata
     3001 ttgataatgg atttggagca ggatatttag catcaatgat aaataaactg taaatacatt
     3061 gatataaatt aaacaaaact atataataaa atttaatttc tattatattt ttactattaa
     3121 aattgaggag acatatatga gtgaaaaaat actttatttt gatataataa gcggtatatc
     3181 aggggatatg acccttgcaa gtttattaaa tttaggtgtg cctaaggaaa tttttttgga
     3241 ggaaattcat aaactaagaa tgagcgatga atttaatata aacatcagct cgaaaactga
     3301 aaatggaata gtaggtacaa aagtagaagt aattacaaaa gaaaaacata cacatagaaa
     3361 tttagtggat atatttgagg ttatagataa aagcactcta aacgaaaatg taaaatctaa
     3421 agcaaaaaaa atatttatgg taattggaga agcagaagca aaagttcatg gaactacaat
     3481 agacaagata cattttcatg aggtaggggc tatagattca attgtagata tagtaggagc
     3541 ttctatatta gttgacttac tttgtgtaga taaagtttgt gctagtacag tgtctgttgg
     3601 ttcaggattt gtaaaatgtg aacatggaat tatacctata cctgcacctg caactataga
     3661 aattttaaaa ggtgtgccaa ttaaattaaa taatgtaaat ggagaatgta caactccaac
     3721 aggagctgca atcataaaag ttttgtgtga tgagtttgta gatgagcttg aattttctcc
     3781 aaagcaaatt ggatatggta taggtcataa gaagtttgaa gtaccaaata tactcagaac
     3841 tgtattaggt gaaaaaaaaa agaagaatta atttatgaaa ttagtgcaaa cattgatgat
     3901 atgtcttcag aaatctattc ttatttgtat gaaaaaatat tagatgagaa agcattagat
     3961 atttatacag agagcattta tatgaagaag aatagaccag caacaaaagt atcaatttta
     4021 tgtaatgaat cagatttaga aaaatttatt aaaatattac ttttagaaac tagtactttt
     4081 ggagtgagat ataatgcata taatagagaa attctagaga gaaaatttgt aaaattagat
     4141 acaaaatatg gagtggttac agtaaaacta gggtattata aaggaaaact agtcaaggct
     4201 actccagaat ataatgaatg taaaactata gcaaaaaatg aagatattcc tataaaaaaa
     4261 gtgtttgctg atataaattt tagaataaaa gaagaattta gtgaaaactt attgacataa
     4321 gtaataaatt ttgataaatt aatattcagc cttattaaat aaaagtaaac agaaaaaaat
     4381 atataaaata taaactcact cgtatatgct cagaaatatg gtctgagagt ctctaccaag
     4441 ataccgtaaa tatcttgact atgagtgaaa ttattatacc aaaagaccta gttaagtaaa
     4501 tgtgtaggct gagttaaact ccctatgtgt tggctataag ataaaggtat tttggaagcc
     4561 tgtatagtaa tttcactttt gctagaaatt atggaagtta ctatacaggt tttttgtgta
     4621 aaaagattta atataattta gactgtattg tgttaaaata aatatataaa aactatatta
     4681 tacaaggaga gatgtgaatt tatgaaacat gaattagtac ttgtaataga ttttggagga
     4741 caatataatc agttgatagc tagaagagtt agagaaaaca acgtatattg tgaaatactt
     4801 ccatgtactg caagcataga aagaataaaa gaaaaaaatc ctaaagggat aatatttaca
     4861 ggcggtccaa atagtgcgta tttagaagat tctccaacaa tatcaaaaga aatatttgaa
     4921 ctaggaatac caatacttgg aatatgctat ggtattcaaa taatgtctca tgtattgggt
     4981 ggagttgtaa gaaaaggtaa taaacaagaa aaagaatatg gaaaaacagc tataacttat
     5041 ggaaaatcat cattatttga aggaataact acaaatagtg tatggatgag tcacacagat
     5101 ttaatagaaa aagttccaga aggatttact atagttgcaa atactaatga ttgtccagta
     5161 gcagctatgg aaaatgtaga acgaaaccta tatggagttc aattccatcc agaggtagaa
     5221 cactgtttgg aaggggacaa gatacttaca aacttcttat ataatatttg taaagtaaaa
     5281 ggtgactgga ctacagattc attcatagaa gataaaataa aagaattaaa agaaaaaata
     5341 ggcgataaaa aagctttatg tgctttaagt ggaggagttg attcttctgt tgctgcagtt
     5401 cttattcata aagcaattgg ggacaactta acttgtatat ttgttgacca tggtttactt
     5461 agaaaaaatg aaggaaatga tgtagaaaga atatttagag agaaatttga tataaattta
     5521 ataagagtta atgcagaaga tagattctta tctaaattaa aaggagtttc tgaaccagaa
     5581 gctaagagaa aaataatagg tgaagaattt ataagagtat ttgaagaaga atcaaataaa
     5641 ttaggaaaaa tggatttctt agtacaagga acaatctatc cagatgttat agaaagtgga
     5701 catggaaatg ctgctactat aaagtctcat cataatgttg gtggaatccc agaagatgta
     5761 gacttccaag aaattgtaga accattaaga gagttattta aggatgaagt tagaaaaata
     5821 ggattagagc ttggtataga agaaggatta atatttagac atcctttccc aggaccaggt
     5881 ctaggaataa gagttatagg tgatgtaact aaagaaaaat gtgatatatt aagagaagct
     5941 gatgctgttt atatggacga gcttagaaaa gcaggtttat acagagaaat atggcaagct
     6001 tttgcaacat taccagatgt taagactgtt ggagttatgg gtgatgaaag aacatatgct
     6061 tatttagttg gtttaagagc agttacttct tctgatggaa tgacttctga ctggtataag
     6121 atgccttatg atgtattaga aagaatttct aatagaataa taaatgaagt tgatggagta
     6181 aatagagtag tttatgatat aacttctaaa cctcctggaa ctattgaatg ggaataaaat
     6241 atagaatgcc aaaaatccac ataaatacag gatttgaggt ctctcttact tctatgttat
     6301 actggtttga tactatcaat atttgccagt atcttatgga ttaataagca aaaaacatcc
     6361 tgctgaatga taaattcagc ggggtgtttt tattttgttt tagaactcct ttgctctgaa
     6421 actcgaagta tttaacactg atgatgttgt tatcttttag ttccttttgt gtcatgctct
     6481 gaaagacaaa ttgatacaat aaaaaagtaa tttcttaatc attgatggag atatgtgttt
     6541 atgttactct tgatggtaat ttgagtaata ttgttcatag ctttttatat tcacttaatt
     6601 agaaaaccat ttggtataat cataaataaa cttgatgata taatagaaaa gatttataat
     6661 tattagaagt atataaaagt attttatgtc tatgaaatgt atgtctataa aacatataga
     6721 tgacttttgt atattaatgt atgaatattg atattataga agaattgagt tgtcaatgat
     6781 actaaatcaa atatttatag aagctggagg gattatgaga aataaaataa ttataggaat
     6841 tactttttca cttgtattac taatgtctgg atgttcttca aaacaaaccg cagaaataaa
     6901 cgtattagca actactgatt tacatggagt tataccctat gagttaactt cttatgtaaa
     6961 agaagaaaga aaaaaagata aaaatctaac tttagtagat gcaggagatt tttttgatag
     7021 tggagaagtg tttggttcta gcatggatag ttattttagt gctagatata aagatggtga
     7081 taataacact gaaagttata ttgagacgcc tatttcaaaa gatatgaaag aggtaggtta
     7141 tgatgttgca gttcttggga atcatgaatt tatatccaac aataaatttt atctagataa
     7201 tatgatatct gattttgaga agcaaaaaat aaatatttta tcggcaaata cgtataaaaa
     7261 aaatggagat agttatacaa aaccatacat aataaaaaat attaatacac ctgaaggaaa
     7321 cgtaaaacta ggaattttag ggcttactat aaaagaagtt ggtgagagga aacagtggaa
     7381 agatggaaaa cttgttgact ctaagtcttt agaactaaaa gaccaagagg gttacaatgg
     7441 tgaattgtat atgaatgatt tagtagaaga tgctaaaaag tgggttggta taatggaaaa
     7501 agagaagcct gacattatag ttgctgtggc acatacaggt gagaaaccta agaaacctaa
     7561 aaacccagga aatagaatac aagacttagc tcaacaagtt gatggaattg atgctatagt
     7621 tgcaggtcat aatcatgttc aaattgagca acatgattat aaaaataaat caggagaaaa
     7681 tgttatagtt actgagccag gaaagcatgg ggaatgtatt tctaagatta attttaaatt
     7741 agaaaaaaac aatgataaat ggaatatagt tgacaaatct agtaaattaa ttaaatttga
     7801 aaaaaatcaa gaaagtgata aatttggaga atttatttct tcaattaata aagtaagtaa
     7861 agataagaaa gaaataagat tatcagaagt aaagactcct gaatgggata aaatatatgt
     7921 ttttaaatct ggaacatctg cagatgatat atataaaaca ataggatata aatggagaaa
     7981 tatatcatct ccaaagggtg atgacatggt gcagattgtt gtaatgaata ataataagcc
     8041 tgttagctat gtatatggtg aagctgaagt tatgccaatt aatataaaat gtgatgaatc
     8101 agagtataaa gataatgttg taactattta tcctaataaa aatgataaat ttaaaattga
     8161 aaaaggtaaa caagaatata gtattaattt aaaacatata caatagaaac ttttatctta
     8221 tttaagttta gattgtactt aaaataaagt gtgtattatt ttaaaaattg atgataatta
     8281 aaatcacaac attaaggaat agtacacact ttttatgcta aaaaatagaa tgttaaaaaa
     8341 ttaaaccttt aaattaaagt acattgctta tattatccaa tattattttc aaaggttgtg
     8401 taatattttt atccttatgt aatattgcta acattttatt ttccatatct atcccatcta
     8461 attcaatcaa tcgcaatttt ttatgtaaga gtgaatctaa taataaattt tctggcaaaa
     8521 tggttatacc taaccctgct tgtgcagctt taataagtgc aaaagaatta acactttccc
     8581 aaacaggata agctttttgg tttgaaagag ataatacact gtccaaagta tcgcgaatgg
     8641 cacttccttg ctcacgtaac aataacggaa acgtgcataa ctgtcgtaga gataaggctt
     8701 gttcagataa aggaaaagtt ggagcacatg caacacataa cttataggaa cctaataaaa
     8761 ttgtacggaa tgccccctgt ggttctatac cttcccaaaa acatatatca gcttctccac
     8821 tttttaaaat atccatagct gaattagcac tttcgacacg tacattcact ttaagagatg
     8881 ggaatgaatt ttttagtcga cttaaaattg gtggaagtaa aaaagaggca attgtaatgc
     8941 tagaaacaat gtttataggt gtacattcct ctaaatgact gattcttttg tctaaattac
     9001 gacaagcagt taagatacta cgtgcttctt ctaaaagaga aatgccacag cgagtaagac
     9061 atacccctcg tggtagtctg tcaaataatg ctgtttcagc ttgttgttca agttcagcta
     9121 tagcatgaga tacagcagat tgtgtgatat gtaatttttt tgcagcacct gtaaaagtgc
     9181 ctgtttgttc aattgcttct aaaatctcta aatatcgtat gtttatcata ataaatatcc
     9241 tcactagaca tgaataaaat tgattaattc cattaaattc tatcatttta cagattgcaa
     9301 agcaacaggt atactgaata atatgaagtg aaaagaagga ggaatatttt gaatcaaagc
     9361 agaaaaacat atttttggct tttaattatt aacttgttta ttagcacatt cacttttggt
     9421 ggagggtatg tagttgtacc tatgattaga cgttattttg taaatcaaaa gcaattcttt
     9481 acagaagatg atttgatgag catggcagca gttgcacaat caactcctgg agctattgca
     9541 attaatttat ctgctttggc aggttatcga gtagctggta ctttaggggt tgtcattagt
     9601 tgtatagcag ctataatacc tccactcgtt attcttggat tagtatcagc attttacacc
     9661 acttttatat ctaatgccat tgtaactgca gtattaaaag gaatgcaagc tggtgttgct
     9721 gcactaattg tggatttgat tattgacatg tgctctatga tattaaaaca acgttctcta
     9781 tttttatcag ctatgatacc tattgctttc tttgcaaact ttgtgatgag tataaatgta
     9841 atttttatat tatctgtttg ctgttttgta tgtgtaatgc aagctttcta caagaagagg
     9901 gaaaataaat gaatacaatt tggaacttat tttttatatt tttaaaaata ggattgttta
     9961 gtattggcgg tggttatgca atcatacctt taatccaaga gaaagtagta aatcaaactg
    10021 gatggataag tgagaaaatg tttacagata ttataactat ttcacaaatg acaccagggc
    10081 cactggctgt taatacttct acttttgttg gacttcaaat tggagggttt tggggagcag
    10141 ttgcagccac tttaggttgt gtattatgtg gtgtagtaat atctctattt ttatataatt
    10201 tttttcaaaa gcatcagaac tctacttata tatttgaaat attaaatggt ctaaaatcag
    10261 cttcattagg tttaattatc tcagctacgg ctacaattat aatccttgca ttatttggga
    10321 cgaataaagt gtacttcaat tctatatttg agatgctaaa ctggactgca ctaataatct
    10381 tttttgttac actactgata gtacgtaagt ggaaaattaa tcctattgtg attataataa
    10441 taagtggtat actgggaata tttttttatt tataagtttt tggtaagtaa aggatatcta
    10501 tttttttata tcttaaatcc aaatagccaa taaaataagg ctatttggat ttttaataaa
    10561 ttctgaaaag ataatcagaa acccatataa catttaaatt atatcactgg ttttctacaa
    10621 accaatcata agcttcttgg caaaatttct cactatctat atagtaacta ttatgaacat
    10681 tattaatttc aacaagtttc tttttacatt ttagtgaatt ataatagcta tcaaaatatt
    10741 gggttttaaa atatctatca tgaagtgttt taaagacaag agtaggtatg ttgactccat
    10801 cttttatctt actatcttct gcaataaagc attctaacat tgtattaatt atctttgtac
    10861 tatttttcca aatatgagga tttttctttt caaatatttt ataaaattca ttatcataga
    10921 cttcattata acctttatat ttaggtggtt ttactgttat gtgaaaatta ggaagaaact
    10981 taaagaaaaa atataataat ttttatgttc tgttttatat attattcata ttttatcaca
    11041 gataatatac taaagttaat ttttttattg tacaactatt ataataattc ttctataata
    11101 taagacattc tataatttta tatttaagtt catgtgtatt taattgtttt tatataaaat
    11161 gtccagtaat actatttgga tttattttta tttcatcagg agttccagaa gcaacaattt
    11221 cccctccatt tattccactg tctggaccta tatcgataat ccaatcagaa gcacgtataa
    11281 cctctgtatt gtgttcaata attaaaagtg aatgctgttc atttacaagt tttctaaaag
    11341 taattaacaa attttctata tctttaaaat gaagtccagt agtaggctca tccaatatgt
    11401 acagcattcg ttttttagtt tttgtcccta attcttttgc taattttatc ctttgagatt
    11461 ctcctccact taatcctaat gtattttgtc caagctttag atatccaagt cccacttcat
    11521 ctaataagga gagttttcta tatatatcag agcaatcgct aaaaaatgtt tttgcttctg
    11581 ctacttccat gtcaagtata tctgcaattg tctttccctt gtattgtata gacagtattt
    11641 ctttttgata tctcttacct tgacattcac tacaagttac ccattggtct gccatatatt
    11701 gcagaataat ttttgtttta cctaatcctt gacagtttgg gcattgcccc tttttgctat
    11761 taaagctaaa atagctttca tccaataatt tttgttttgc ttgtggagtt tcagcaaata
    11821 gagaacgaat ttcatcaaaa atattgatat atgtagcagg tgtagaacgg ctagattttc
    11881 caataggtgt ttggtccatt aagataaaat catcaatagc atcaaatcca gtaaattcag
    11941 tataattttt atctgggata gatttttgtt tcaatttttc ttctagagca ggaagtaaac
    12001 tgtgaaatac aagagagctc ttaccagaac cactaacacc tgtaatagag cacatacagt
    12061 ttaagggaat ttctacatca atatttttta agttatttgc atggcatcct tttaagctta
    12121 accatttact tggtatagtt ttattttgtg aacttatttt attgtatgtg cttaaatatt
    12181 tacctgttat agagtttgga ttttttataa tttcttgggt tgtaccttct gcaataatga
    12241 accccccttt tgtaccagca cttgggccta catcaataat ataatcagca gacagtattg
    12301 tatctttatc gtgttccaca actataacag tattaccctt atctcttagc tcttctatca
    12361 ttttgatgat taaattatgg tctcttgggt gtaaaccaat agatggttca tctaaaatgt
    12421 aggttaagcc tactaattca cttcctagct gacttgataa tcgtactctc tgcagttctc
    12481 ctccagataa agttggagct gttctatctg ttgatatata gctaagacct acctttaata
    12541 gataactaac tcgaattttt aattctgtta agatatcatt taccaaagag agctcatttt
    12601 tttgaagttg atttggtaat tcacacaacc aattccataa ctgttctatc gtcatttttg
    12661 ttagttcagg gaagcgataa cctttgattg tagtgaatct agcctcaatg cacagcaatt
    12721 cgccaccaca tgtattgcaa ggaattttgt tcatatattg atgcaaggta ttattttcag
    12781 aactagattc tctaaaaagt ctttgaatgt ggttgattgc tcctgaagca ggtcttctaa
    12841 tctttgattc tcgtcccttt gattcaaagc taaattcata tatcttatcg tctgtaccat
    12901 ataagattgc atctataaaa tctcttggta attcattcca aggtatatct aggtctatat
    12961 ttttatcttt tgctattgca tataattcac ctaccatcca attaccattt ggctttttcc
    13021 ccctcaattt tccaaaatag taagtagctc catctaatat tgaaatagtt gggtctttta
    13081 caatcaattg aggattaact tgataggtaa aacctaatcc attacaatcg tgacaagctc
    13141 cagtatggga gttagcattg aaggttgaag tatttatctt agaaaaaata gtttgacaat
    13201 gtggacaata attttttgta ttattctttt tttctaatgg aattttacaa tatggacagt
    13261 aactttttcc gattgataca aataacattc ttaaataatc ccaaatgctt gtaagagtac
    13321 caacagtaga gcgaggattt tggcttcctt ttttctgttc gatagctata gtaggggtta
    13381 atccctgaag taaatcaaat tccggttttt gaagctgttc tgaaagtttt gctccattac
    13441 ttagattttc aaaataacgt ctttttcctt cttcataaat cgtatcgaaa gctaaagaag
    13501 attttccact accacttaca cctgtaataa cagttatttg attttttgga attgtaacct
    13561 caacatcctt taaattgttt tgccttcctc cttttaaata gatagagctt ttattatgac
    13621 aaagtgaatt tttaattgat ttatttattg attttgtaaa tttgaatttc ttttcgagtt
    13681 caagaggatt gcctaattct tttatttccg aaatgcctaa tatttttaac tgagaaaaga
    13741 ttgatttcaa ataatcttca ggcaattttt ctattaacaa attaaggtct tctattttta
    13801 tatttccgct tgataatatt tctgccattt catctaataa atttttttct gctcttgtta
    13861 attgattgta ttttttggaa cttaattctg tccataaatc ccatggaagc aattctaaac
    13921 caatcatatt attaaagtca tgtagaagat taccttttac agaatgccaa cctttccaat
    13981 ttttattata tccaaagtca tctggatgag catgtccttg ccgacatctt atccaggctt
    14041 ctgaaccagt taaaaaacca tctgtttttc cgacatcatg agtgtcaaaa ttaatttgaa
    14101 aataattttt ttgtatgtca tctatttgtg catctattct tatccatctc tttgttagag
    14161 tatcatagta ttctacaagc cagtggtctt cataagtaat ctcactttca aaataattag
    14221 caaaacctgc tctcattcta gcctcaaaac ctcgatatct taggagcgat acaagaagta
    14281 aagaataatc tctgcacatt ccaacaagcc tttgttttgg gtttcttgga atagttagtg
    14341 ggttagaatt tatttcaaat attcttttta acatttgagg aattgtacga agcaattcct
    14401 catcagattg ttgtttatta aaggatatac catataattt tccttggtct ccatgaatta
    14461 ctaggctttg gaccactttt aataactcaa ttggagtatt tggtaaatct tggtatttat
    14521 tttcaaattt tttaggattt gtgatgatgc tttgattttt atagttattt attttcattt
    14581 atatttcacc tctctaaatg gatgatatac cctgacatgg tgtcatatgc aaggacaatc
    14641 tagatttttt ggccaaaatt taaaaataaa taaaaaatga tacagtgat
//
LOCUS       DAEORZ010000106        14396 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEORZ010000106 DAEORZ010000000
VERSION     DAEORZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.107"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            228..428
                     /locus_tag="KRH39_002754"
     CDS             228..428
                     /locus_tag="KRH39_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG8512643.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            complement(608..1486)
                     /gene="speB"
                     /locus_tag="KRH39_002755"
     CDS             complement(608..1486)
                     /gene="speB"
                     /locus_tag="KRH39_002755"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBG8512644.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYKEFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(1476..2327)
                     /gene="speE"
                     /locus_tag="KRH39_002756"
     CDS             complement(1476..2327)
                     /gene="speE"
                     /locus_tag="KRH39_002756"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBG8512645.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            complement(2357..2770)
                     /gene="speD"
                     /locus_tag="KRH39_002757"
     CDS             complement(2357..2770)
                     /gene="speD"
                     /locus_tag="KRH39_002757"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBG8512646.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            complement(2857..4332)
                     /locus_tag="KRH39_002758"
     CDS             complement(2857..4332)
                     /locus_tag="KRH39_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG8512647.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            complement(4970..5623)
                     /locus_tag="KRH39_002759"
     CDS             complement(4970..5623)
                     /locus_tag="KRH39_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8512648.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(5868..7733)
                     /locus_tag="KRH39_002760"
     CDS             complement(5868..7733)
                     /locus_tag="KRH39_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBG8512649.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(7738..10158)
                     /locus_tag="KRH39_002761"
     CDS             complement(7738..10158)
                     /locus_tag="KRH39_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBG8512650.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCGNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(10172..11614)
                     /gene="glgA"
                     /locus_tag="KRH39_002762"
     CDS             complement(10172..11614)
                     /gene="glgA"
                     /locus_tag="KRH39_002762"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBG8512651.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(11643..12773)
                     /gene="glgD"
                     /locus_tag="KRH39_002763"
     CDS             complement(11643..12773)
                     /gene="glgD"
                     /locus_tag="KRH39_002763"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBG8512652.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(12775..13935)
                     /locus_tag="KRH39_002764"
     CDS             complement(12775..13935)
                     /locus_tag="KRH39_002764"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBG8512653.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         4586 a         2529 c         1450 g         5831 t
ORIGIN      
        1 ataccttttt gttacatttt ttgatattaa atttattatc tacatttaca ttttcgtcaa
       61 attttacaat acttctcact gtataattta acaattttta ttaaattcta aaagtaatta
      121 atttttttaa aaattttatt tgcttttatt taaaatttat gttatattaa tagaacagac
      181 ttattttttt agtttgtatt ttttaataat gcggaggttt tcttataatg aaaaacggaa
      241 tagtaaaatg gtttaacaat gaaaaaggat ttgggttcat atctgtagag ggagaagatg
      301 atgttttcgt acatttctca gctatacaaa atgatggata caaaacttta gaagaaggcg
      361 aaaaggttag ctttgatata actcaaggta atagaggacc tcaagctgaa aacgtaaata
      421 gaatataatt atttaattta aatatatata ttttagaaaa ggagctatcg agggctcttt
      481 tttgttgttt attatattta tttttaatta caaaaataag aagttacttt agaaaagcgt
      541 caaacttaaa ctaacttttt taaaataact tcttatatta taatatatac tatttatata
      601 aaatactcta tttaatttta tcagatatta ttaagcataa ctctcttaag attttacaag
      661 ctataactgt cgatacacct gttgtatcgt aatcagggct taactctaca atgtcacaac
      721 caactatatt tatattagag ttttttataa tcttaaaaat ctcttgaaac tctttataat
      781 ttacaccacc aggttctggt gtacctgttc ctggaaacac agatgcatcc aatacatcta
      841 aatctatagt tagataaata ttctttccat ttagcatatt aactatattt tcaaacgtat
      901 ctattcctcc tatttccatg tatgtgtgtt tttcttctgt agcaaattta aattcttctt
      961 ttgtcccaga ccttatacca aattgaaata ttttattatc acctacaata tcccatattc
     1021 ttttaattac tgttgcatga gaatttttac tattattata ttcttccctc aaatcagtat
     1081 gggcatcaaa atgaattaca tatatatcat tgtacttttc atgtactgct ttaaaggctg
     1141 gtaatgtaac taaatgctct cctccaatca taaagggtac ctttgaatct ctaacaatct
     1201 tatatgtctc ttgatagatt tcttttaaga cttgttctgt acttccaaca ctaatttcta
     1261 aatctccata atcacatata ttataatcct ctaaatccaa atctaaaaaa ggactatatg
     1321 tctcaagacc gtaaaattct tttctcatag agctacttgc aaatctagcc ccaggtctgt
     1381 tagaagtagt tccatcaaat ccaacaccaa atactataag attagattct tcataatttt
     1441 tgtccatact cataaaagtg ttcatatgat agaaattatt cttcatctag catctccttc
     1501 acgtattgag gtaacataaa cgaacctaaa tgtatatcac tattataata tttagttttt
     1561 aaacttaatt tctcccatcc atttctatct tgatttttta ctggattaag tttttttgaa
     1621 gcaaatccaa acaaccagtg accagatgga tatgttggta tatgtgcttg ataaacttct
     1681 gatattggaa atatctgttt taatttttta ttagctcttt tcatctcttt agcattaaag
     1741 tcaaaatacg gactttcact ttgatttact aaaattccat catcagtcag tattctataa
     1801 caatccttat aaaaatcagt tgagaaaagc ccttcacctg gtcctattgg gtcagttgaa
     1861 tctactatta tcaggtcata tgatttatca tgtgcacact taacaaaatt aacaccatct
     1921 tcaaaatata aactcactct tttatcatct aatttacatg agcatatatc catatactcc
     1981 ttagatacat ctacaaccat tttatctatt tcaaccatgt caatcttttc tatttgagga
     2041 taacggctta attctcttac tgtacctcca tcacctccac ctatgactaa tactttcttt
     2101 atgtttaaat ttgtagccat aggaacatga gttatcatct catggtatat aaattcatct
     2161 ttatatgtta ccatcattaa tccatctatt gttagaaatt ttccaaattc atcactatca
     2221 aacacatcta tcctttgaaa ttgagacttt ccttcaaata gatgtttatt tacttttata
     2281 gaaaatctta cattttctgt ccattcttct gtataccaaa gttccatata ataatcctca
     2341 ctttctcttg tatgttttat actgcttctg gtttatgagt aatcttacct aaatcttttt
     2401 ttgaatattt tttaattttt gtagttaagc ctcttggaat ttctgtagac tcgcttcttt
     2461 cagactttaa aagttgctct aataattcaa aacttttcca tgggtctata tcaccacaag
     2521 taaaaaaatc cgctgctgca tagccatatt caggccatgt atgtattgtc aaatgagatt
     2581 cagcaattat aacagctcct gaaacccccc aaggattaaa atggtggaat acagaatcca
     2641 caattgtcgc ttttgcattt aatgctgaat cattcataaa attttctata agacgtgggt
     2701 ctttaagtac gttttcatca caattatagt attctactag tatatgtctt cctagtgttt
     2761 caaatttcat ttcaacccca actcctttat acttttaata agttctattt gataaactct
     2821 acttaacaaa tacttgccct tgcaaaataa agtaatttat tttattacta atattctatc
     2881 taagttttta tcttgtacat ctgtaagata tgcattgcta ttttttaaga aaataatata
     2941 ctccataatt tcttctgtta tcaattctcc tggagcaata atcggtatac ctggtggata
     3001 tgccattatt gattctccac aaattctatc tatacatgaa ttgatttcta cactttcctt
     3061 tggtgcataa aaagcttctc ttggattaag tttaataatt ggatttattt gaaccatatt
     3121 aaattcattt aatgttgatt cttttctata aatctttgca attatagaaa gtgcttttac
     3181 taatctatct atatctgatt tatttgttcc aatggacaca agtgccagta tattatatga
     3241 atctcccaat tctacttgaa ttgaaaaatt tttatataat aaatcgtata cttcaaatcc
     3301 agtcaaattt aattctttta catttataca caattttgtt tcatctataa atttaacacc
     3361 tttttgtttc aatatttctg tagataaaac ttttattcca tcaattttgt tcaatttaga
     3421 ttttgcatac ctagatagat ttaatgcttt tgacaattgc ttttcaccat tctcaactag
     3481 attaactcta gcaccatcta tacttgccat aagcaaataa gatgctgatg ttgattgtag
     3541 catattgatt gattgaagaa ccttttcaaa acttacatta tctctattca ataagagtgc
     3601 tgaagattgt gtcaatgctc ctccagtttt atgtatactt acagctacca tgtcagctcc
     3661 caaacttatt gcagaaggag gtaaatctaa atgaaatgga aaatgtgcac catgagcttc
     3721 atcaactaac actaaaacat tatttttatg gcatagttct ataatactct caaggtctgc
     3781 acaaactcca tagtaagttg gattaaggaa aaataatgct tttatattac aatctttttc
     3841 tatctctgtt ctaacttttt caagagtaat atttaaactt atacctagtt tttcatcaaa
     3901 ttctggttgt ataaatattg gtattcctcc acaaagtatt aacgcattta ttactgattt
     3961 atgtatattt cttggaagta atactttttc acctggtttt ataacactca aaatcattgc
     4021 atgtattgct tgagttgtgc cattcgttat aaagaatgcc tcatcagcca tatatgcttg
     4081 tgctagcaaa tcttgtgcat tttttataat cccatttggc gcagatacat tatccatagt
     4141 tggtgatgaa tttatatcca ttttcatgag actttcacct agatatttat ttaatatagc
     4201 tacacctcta ttttttacat gtccaggcac atcaaaacaa gctatatctt tctctgagta
     4261 ttcctttaac gactctaata atggagcaca ctcatagctt aattcaactg catttgcaaa
     4321 ctcgctatcc atgatttcaa attcatctcc tctctatatt caaatatttt taatatttat
     4381 tatttattat ttctattttt tatttttact tttattaaac tcatgatatt tattatatta
     4441 acatcccagc atttttacaa gtgttttttt gaagttactc aaaaacatat acattagcta
     4501 tatcaatatt tataaacttt ttaatttttt gttgtttata ttaatttttt taaaacttag
     4561 tatttataaa ctttcttatt ttttattatt ttggtttttt tacttttttg agttttagta
     4621 agcttttgag tttactattt ttaatttata agtattttat tactaaactt ttatattatt
     4681 cttatttcac atatcaaaat tcttggttta gttggactaa ataattttcc tatttttgta
     4741 ttttaatttt aatataaaat ttattaaatt ttttaattga taaaataaat tctatattta
     4801 tgatattaaa attctataag ttcattgatt tatatatgtt aatttaaata cattattcta
     4861 tcgtagtgcc ttagagtttt tatttgtatt taaagttaaa tttattcttg tttttccttt
     4921 aatatttaat agtaaaacaa caaaattata aaaaaaataa agagaaactt tataaaagct
     4981 tctctttctt catctctgct ataggtatcg ttttccaatg ttctctcaaa actctttcaa
     5041 cattttctaa atcattctcc ctcatagatt ttactacttc aatatgttga ttattgctgt
     5101 atgtcaacat tttatataac tcatcttcat tatcactagt atatgacttt gacataaaat
     5161 tatatcttaa agagttcaat agatttacta acacctgatt atctgattta gttatgtata
     5221 tatcgtgaaa ttcattactt aattttaaat agctattgta atttttatac tttatagaga
     5281 ccgccatcat ttctgttaat tcttccattt ttactaggtc tgattcagtt aaattttgta
     5341 atgctgaaga accggctaac gcatccaata ctccaattac ggaataggtg tctaatttct
     5401 ccttttcctg aaattctttt actacaaatc ccttattagg tattttttct agtaaatttt
     5461 cacctgctaa cctagttaat gcttctctcg ctggtgttct actaactccg atagatttac
     5521 aaatctcaga ttcagtaatt ctctgattag cttcatattc tccattttgg attttctttg
     5581 atatatagtc atatacttga gttgtaagtg gatctacctt cataagacgt tcctccatat
     5641 taagattata aaagttaaat ctccctacaa ctttggtaga gagttcagtt ttatcttact
     5701 aatttatata atatcacaat tatgatattt tttatacttc tttgtaatat tttgaaatac
     5761 taaaattctt atgataattt atacaaaaaa agtagaaaaa gttttgataa aacaaaactc
     5821 tttccacatt cgccttattt tctatttatt tttctaaaaa aagatattta atttactcta
     5881 aaaattctaa atgatttaga gtctaaatct actttcaact cattattttc aatttgtata
     5941 gtactatttg catttttaag actatacttt ataggtattt cctcaataaa actactctct
     6001 atacataatg atatactttc cacattttca cttctattta ctaaaattaa cattctatca
     6061 ttattttcat taactctaat aaaagaaaat acagaatcat tattagtata tataaattct
     6121 gtatttccag aacttaaaag tttattctta tttctaattt ttataacatc tctataaaaa
     6181 ttaagcatat cttcgtcttc atttttccat ggataagttc ttctattatc tggatcaaca
     6241 cctccacaaa gacctgcttc atctccataa tatatatatg gtactccttc aaatgtcatc
     6301 tgtatagcta cagcaagctt cactaaatct acatcatcat ttaattccgt tttagctctt
     6361 gtaacatcat gtgttccaat taaatttaaa ttagacctaa atgattcttt tggataattt
     6421 tctttaatct gcatatatct attacaaagt tcatatgaat taatttctcc ttttaagaaa
     6481 gaaaacatgt tatttctaaa aggataaccc ataacagaat ctaattcttc acctaataaa
     6541 taacttcttc tctgaccata gctaatctta tttgatgcat cttcccatac ttcacctata
     6601 agaattgagt catcatctgc cttttttaat tcctttttta actctcttat aaacttagtt
     6661 ggtaattcgt ctgcaacatc tagtctccag cccttaatac ccatatttac ccatttattt
     6721 ataacgctat cattgtcata tattatataa tccatatagc tatgttctaa ttcatttata
     6781 tttggcaatg tcttaacatc ccaccaactc ttatattttt gaggaaattc ttcaaacata
     6841 taccatgaat aatatggaga ttctttagat tgatatgccc ctaaactatt atagtttcca
     6901 tacatattaa aatatttact atctgctcca gtatgactaa aaactccatc taatactatt
     6961 gatatacctt tttcttttgc tttatcaata agttctttaa atgtatcttc atccccaaac
     7021 attgggtcaa tttttttata gtcacctgta tcatacttat gattactaga ggcttcaaat
     7081 atagggctta gatatagtat acttacacct aattttttta aatatccaag tttattgatt
     7141 atccctctta aatttccacc atggaaatcc catcttataa catccccttg actatccttt
     7201 atgtacatag gtgtatcttc ccaatttcca tatatgaaac tatttttttt aggattatca
     7261 accttaccac tacgatttcc attattgaat ctatctacaa atatatgata aagtattcct
     7321 tctttgtacc atgttggtac tttaaaatct ttgtaaacag tgatttgata tttattaata
     7381 tcactataat tatattcaca agaatatcca ttatctctgt tttttccata aaataaagtc
     7441 tttatctgac catccatttc aacatcaact ttaaagtaat aaaaataaac acctgtacca
     7501 tcaaactttt caatcttacc acaaaagcac tttccaagtt tatcattatc taattcttca
     7561 tttaaagtta ttgttctcat ttcttcttct ctatttattt ctagggaaat agacttgata
     7621 ttgtatccct catttacttt aacacaaatg gtcaattcct catcaaattt taaagctcca
     7681 aacggagcct taaaattttt atcccaagag ttatattcta ttacactttg catttaatca
     7741 caccctttta aaccaaatgt cttttgcata ctcagaaatt gttctatcag aagaaaaaaa
     7801 tccagagtta gctatattaa ctaaagacat tttattccat ttttctttat ctctatataa
     7861 cctgtttata tctgcttgag cttgaccata attttcaaaa tctctcaata caaaatactc
     7921 atcattgtaa gtagtgagtg aattataaat acttcttcct tcttcgccaa gattaggaat
     7981 aaatccattt attaaatcat ctactaccct ttttatatcc atattagaat gatataggtc
     8041 aagtgaagaa tatccaccat atttattata atttagtact tgttctgcac taagtccaaa
     8101 taaaaacata ttttctttac caacctgttc acatatctct acatttgcac cgtcaagtgt
     8161 agccaaagtt atagcaccat tcatcataaa cttcatatta cttgttccag atgcttcctt
     8221 agttgtagtt gaaatttgct cactaacatt tgctgctggt attattattt ctgctaatga
     8281 tactccataa ttctctaaaa atactacttt taacttgtcc tttactctaa catcattatt
     8341 tatagtactt gcaacagaat ttataaattt aattatacat ttagccaaat aatatcctgg
     8401 tgctgctttt gcgccaaata taaaagttct tgggtcaaga ttcaaattag gattatcaag
     8461 taattcatga tacatatgaa gtacattaaa tatattcagt aactgtcttt tatacgcatg
     8521 caatctctta acttgaacat caaatatcga acttgggtct acatttaaat cgtatttatc
     8581 ttttataaac tttgctagat ttgctttatt attgtatttt atatcatcaa gtctttgtaa
     8641 aacagaggaa tcatttttaa atttttcaat attttttaat tgtaatgtat ctttcttcca
     8701 tgaatcacca attaaatctg taattaaatt gctaagttga ggatttgaac ttataagcca
     8761 tcgcctatgt gcaattccat tagtcttatt attaaattta tttggttcat cttgatagaa
     8821 atcttttaat acctctgttt caagaatatg agtatgaagc ttagcaactc catttacact
     8881 atgacttgtg acaatacata agtttgccat attgatattg ccacagtcta ttatactcat
     8941 tctttttatt ctatcttggt catatccttt attatttaat tcctctacat atcttctatt
     9001 tatttcttct attatcatat aaattcttgg taatagttct ttcatcatac ttacaggcca
     9061 tttttccatt gcttctgaca ttatagtatg attagtatag gacactgttt ttttagttat
     9121 ttcccatgct tcatcccaag ataaattttc ttcatcaagc aaaattctca taagttctgg
     9181 tatgcataag gtaggatgtg tatcatttat atgaattgct accttatcga ataaattatt
     9241 aatatttaat ttgttctttt tatattttct tattatatct tgtatccctg cacttacaaa
     9301 aaagtattct tgtttcaacc tcaacaactt acctgcatag tttgaatcat caggatataa
     9361 tacctgtgat atttcctcag tataatactt atgttttaaa gcctcttcat agctaccaga
     9421 ataatttaga gcatttgagg taagttcacc aaaatctctc tttggtattt cactcttaaa
     9481 caatcttaaa gtatttatac attggttttg gtatccgatt attggtatat cataaggcat
     9541 tgccattaca ggaaggtagt ttttatgaat tacttttaaa tgactacctt ccttgattaa
     9601 ttctacctca ccaccaaact tcaccattgt tgcttcattt ggtcttacag tttcccatgc
     9661 atatcgtcct tctgtaagcc agttatcagg cacttctacc tgataaccat ttacaaattt
     9721 ttgttcaaac agaccatatt tataccttat tccatatcct tgaccactta tgttcaaaga
     9781 tgccatagaa tctaaaaagc aagctgctaa tctaccaagt ccaccatttc ctaaggctgg
     9841 gtctacttca gcttcaatta aatcatccaa gttgattcct aattcagata aaccttctct
     9901 aacttcttct tcaatattta aatttaataa attagatttt aactgtctcc ctattaaaaa
     9961 ttctaaacta aaataataaa cttctttttt cttatctaac tttgtagcaa cccatttttt
    10021 tgctatctca tctttcaata gactacataa tacatttaac aattgttcat cagtagcttc
    10081 cttgatagat tgtgcatata agctatacat ttttacttca aatttttgct tgaatttctt
    10141 tttgcttatt gttaccaaaa aaaccactcc tttaatcaca tatgtctcta tatgtttcta
    10201 tatactcctt agctgatttt ttccaactat tatctgtttt cattgcattt tcaactaatt
    10261 taatccattt ttctttgtcc ttaaatactt tgattgcatt tttcaaacag aaaaacatct
    10321 catgagcatt gtaattttta aaactaaatc cattgccttc tccagtaaat ttattataag
    10381 gtgttactgt atctctaagc cctccagtct ctctaactat tggtaaagaa ccatatctca
    10441 tagctaacat ttgacctata ccacaaggct caaataatga tggcattaag aacatatcac
    10501 ttgcagcata tatctgttga gccaatgatg cattaaaagt aattctagct gatactttat
    10561 ctgaaaaatt agaatcgtaa tagttgaaca ttgattgata ttgatgttct ccagttccta
    10621 aaacgacaat ctgtaaatct tctctcataa gttctggcat catgtatgct attaaatcaa
    10681 atcctttttg agaaactaat ctcgaaacta tacctatcaa cggaatagat gaatcttttt
    10741 ttagtcccaa tatatcttgt aatctcaatt tattttctac ttttttgtca attgagtcta
    10801 catcataatg aacaaatata tctttatctt ttgcagggtc atttaaatca taatctatac
    10861 catttaatat accttttaat tttcctgact cttttctaag taatccatct aattgttcgc
    10921 cataaaaaga tgtttgtatt tcatttgcat aagtaggact aacagttata attttatctg
    10981 caaaatttat accagctttc ataaaattta ttgagccata atactctata tctccattat
    11041 caaagtattt ttgtggtaaa tccaatatat catataaaac atgcttatca aatacacctt
    11101 gatattggag attatgtata gtatacatag tttttatatt tttatacccc tcaagggttg
    11161 catatctttc ttttagaatt aatggtaaca tccctgtatg ccaatcattt atatgtatta
    11221 catctggata aaaatcaatt ctacttatag cttctaaaac tgcatctgta aagaaagtat
    11281 atctttctgc atcatctcca tatccataga tactctttct ttcatcttct cttctaaaat
    11341 aaaattcatt atctataaag taaaatttta caccatctaa ttccatttct aaaagaccac
    11401 agtattgatt tctccatcca acacgtacac taaacacagc tatttctttt agttgatctt
    11461 ttaaataact tggtatagtt gaatatttgg gcattattac ccttacatct acaccttctt
    11521 tttttaattc tttaggcaat gcataagaaa catctcctaa tcctccagtt tttgcaaagg
    11581 gccaacactc tgctgttaca taaaaaacct tcattagtca ttcccccttc taaaaaattt
    11641 atttatatat ttgtattctt ttccactaca agaggatatt tttcatctcc tcttaattct
    11701 ttcccatttg atacactcgt atttttatct aatattgcat tatatagttt tgcatttgac
    11761 tttattacac aattttgcat tactattgag ttctctataa ctgttccttt ttcaacattt
    11821 acctttctaa atatgatact atcttttaca gtaccttcta ttaaacaacc agatgctata
    11881 aagctatttt ctactttagc actatctgta taaattgtag gtgattcatt cttttctttt
    11941 gtaaatatct ttctttcaga atataataat tcatctgcaa cttctaaatt taataaatct
    12001 ttgctcattt caaaatatga ttcaacagaa tttacacatt ttagatatcc tgtatattca
    12061 taggctccta ttttcatgtt ttctgcttct tcaataataa attcttctaa ataacaataa
    12121 ttgcctttat ttacaatgtt atatatacat tcaacaaaat cttcacgctt cataatatat
    12181 gtctccatac atatgtttgc tctagggaac ttacctaaat tatttcctac atttattacc
    12241 ctattgttac tatctaaatt tagagttaag ttaccagcaa aatctttatt tgcattattt
    12301 acatttttat atattatagt tatatcatta tgagattttt tatggtattc caatgcttga
    12361 gaataattta aattgcatag catatagctt ggagctatta aaatatattc ttcttcactc
    12421 ttttctatat aatctagatt agctaagata gtatatatat caccttctct atgtgagtaa
    12481 gtatttctat acttagtgtt ttctggacta aaaattgata ggccaccttt ttttctactt
    12541 aaatcccaat ctttaccact acctatatgg tctgttaatg atctgtattt ttcttttgcg
    12601 aatattccaa ctttagtgat tcctgaattt atcatattag ataaagcaaa gtctattatt
    12661 ctatatcttc cagctattgg tgtagatgct attggtcttc catagttaag tttgtttatt
    12721 gctgggtctc cttttttgtt taaatttatt atccctaaac attcatttct catcttaaag
    12781 acctccttct attaatacgc atctaggttc gatattttca tactcagaaa ctacatttat
    12841 ttcattattt acattcttta tctgaacatt atcatttata gttgcacact cacctatcat
    12901 agctttttct atagttacat tttcacctat tacaacattt ggcattatta ctgaatcaat
    12961 tactttactt cctttaccaa caacaactcc atgtgataat acagagttac ctacttcacc
    13021 taaaattcta caaccatcag ctatcattga tctatgaaca tttgcatttt tacctatata
    13081 ttgtggaggc attgccatag tatttgtata tatcttccac ttagggtctg ctaaatcaag
    13141 tgtttctgga gattttatta tatccatatt agcatcccat agactttgta tagttcctac
    13201 atctctccag tatcctttaa atggataagc atacattcca acattatctt ctaacatttt
    13261 aggtattaaa ttttttccaa aatcatcata atttatttct ggattttttt ctgcttcctt
    13321 aaaataattt ctcaacattt tccaatcaaa tatgtatact cccatagatg ccaaattgct
    13381 cttaggttca cttggttttt cttcaaattc atagatactt gaatcttcat ttgtattcat
    13441 tatcccaaat cttgaagctt catcccaagg aacttcaatc actgctattg tagctttaga
    13501 gcctttttcc ttgtgaaatt ttaacatttt gctataatcc atcttgtata tatggtcacc
    13561 agataatatc aatacatatt ctggattata ggtatctaca aagtccatat tttgatatat
    13621 cgcatgtgct gtaccattat accaatttcc ttctttttca ttcataaatg gttgtaaaac
    13681 atagacacca ccatttattc tgtccaaatc ccaatgactt cccatgccta tatgtgaatt
    13741 taatatcaac ggtctatatt gagttaaaac acctacagta tcaataccag agtttgaaca
    13801 attgcttaac acaaaatcta taattctata cttacctcca aatgatacag ctggtttggc
    13861 aattcttttt gtaaaaacac ctaatcttga accttgccct cctgccaaaa tcatagctaa
    13921 catctctttt ttcatttaat gacactcccc tcatctagta attaatattt ataattgcat
    13981 ttttactttt ataaacttta tcagtcaata aatgtcgtgt ataaatgttg cttaaataat
    14041 accttaataa aaataaaata atatcaacta taacaattga tataatgtat accccataat
    14101 ggacttaccg acgtatacat tattttccat aagcactata gtatttatta atattcaatt
    14161 atcggttaaa aaataataca aattttattt tataaatata aaaacatttt ttataaatcc
    14221 cttgtgtcat ataaacatgc ttatttttta atttgttaaa ttcgtaatag caaaatataa
    14281 tctaatattt gttaaataat tatccaaaga aaatcaagta attattaatc aactttattt
    14341 tttaattgat acttctttgt ctataattta aacaaaagga tataaacttt taaatt
//
LOCUS       DAEORZ010000107        14263 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEORZ010000107 DAEORZ010000000
VERSION     DAEORZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.108"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            210..2087
                     /locus_tag="KRH39_002765"
     CDS             210..2087
                     /locus_tag="KRH39_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8512654.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNVGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKYGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLVG
                     N"
     gene            2444..3850
                     /locus_tag="KRH39_002766"
     CDS             2444..3850
                     /locus_tag="KRH39_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG8512655.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGAVDFSIYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            3878..4726
                     /locus_tag="KRH39_002767"
     CDS             3878..4726
                     /locus_tag="KRH39_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG8512656.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            5387..6079
                     /locus_tag="KRH39_002768"
     CDS             5387..6079
                     /locus_tag="KRH39_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8512657.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            6076..7011
                     /locus_tag="KRH39_002769"
     CDS             6076..7011
                     /locus_tag="KRH39_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8512658.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            7159..8082
                     /locus_tag="KRH39_002770"
     CDS             7159..8082
                     /locus_tag="KRH39_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512659.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            8066..8836
                     /locus_tag="KRH39_002771"
     CDS             8066..8836
                     /locus_tag="KRH39_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512660.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMTFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICIIYKKQEN"
     gene            8846..9628
                     /locus_tag="KRH39_002772"
     CDS             8846..9628
                     /locus_tag="KRH39_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512661.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            10005..10541
                     /locus_tag="KRH39_002773"
     CDS             10005..10541
                     /locus_tag="KRH39_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG8512662.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            10573..10962
                     /locus_tag="KRH39_002774"
     CDS             10573..10962
                     /locus_tag="KRH39_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG8512663.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            10999..11385
                     /locus_tag="KRH39_002775"
     CDS             10999..11385
                     /locus_tag="KRH39_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBG8512664.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKVEL"
     gene            11512..12954
                     /locus_tag="KRH39_002776"
     CDS             11512..12954
                     /locus_tag="KRH39_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG8512665.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            13251..14066
                     /locus_tag="KRH39_002777"
     CDS             13251..14066
                     /locus_tag="KRH39_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8512666.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIITGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLIISI"
BASE COUNT         5152 a         1732 c         2703 g         4676 t
ORIGIN      
        1 gttgaattaa gaaaacgctt atgatactat aaagttaaca aatttgaata tataacagta
       61 gacgtgttac tggtaatgca ggcaagatcg aatcaatttt tatagattat ctatagagta
      121 ctatgggtat aatctattta tttggttcga tttttttgtt taattggcca taaaacaaaa
      181 agcctatagt tgaaaatgga ggatttttta tggattataa aaatatagct caagaaattc
      241 tactaaatgt aggaggaaaa gagaatgtaa atgaagtgac tcattgtatg acaagactta
      301 gatttaaagt caaaagcgct tctaaagtga ataaggataa attatctaaa actgaaggag
      361 taataactgt tgttgagagt atgggtcaaa tacaagttgt aattggaaat aaagtaaaaa
      421 aagtatatga tgaggttata aaaattgtac ctcaatcaaa taacgtaggt aaaaagaatg
      481 aaagtcaaga aaagcaaggt ataattaatt ctatattatc agcagtagct ggtatattta
      541 cacctacaat acctgctata gcaggggttg gtatgattaa aggtatttta tcagttcttg
      601 ctatgtatta tatgaataaa aatggagtag atataaaaga aacgcaaagc tatattatct
      661 taaatgccat ggctgattct atattctatt ttatgcctat tatactagga tatactgcgg
      721 ctaaagtatt taatgcaaat aaaataatat ctatggtatt aggagctaca ctttgttatc
      781 caacttttac tgctttgatg gcaggtgaag aatctgttag atttttagga ttggcagtaa
      841 caaaagctac gtatacatca tctgttattc caataattat tgctatatgg gctctttcat
      901 acgttgaaaa agtattagaa aagtatatac ctgaggtaat taaaataata atggttccaa
      961 ctttatcatt ggttgtaatg ttacctgcaa cattattttt atttggacca atcggaattt
     1021 atataggaaa tgtcataaac ttttcttata agtatattta tgaattaagc ccagcattgt
     1081 gtggagcatt tgtaggtggg ttatggtgtg tgttggttat atttggtgct catagagcat
     1141 tattaccaat aggtataagt gatgttgctc aaacaggaag acagaattta ttggcatttg
     1201 caggagctgc aaacttttca caagcagggg ctgctctagg agtattcttt aaaactaaaa
     1261 atcaaggact aaaaacaatt tctatgtctg caactataac agctttattt ggtataactg
     1321 aacctgcaat atacggtgcc aatcttagat taaaaaaacc tatggtatgt gccgtaatat
     1381 gtggtgcatt tggaggtgcg ataatgggaa tgggtggagc ttatggtaat gcatttgcaa
     1441 accaaggcgt tttaactata cctgtatatg ctgaagcagg tgcattaggt ttcttaagtt
     1501 atctaggtgg ttgtgctata gcattttttg gttcagctat ttcaacttat ctagttggat
     1561 ttgaagattt ggaagagtct tctaataagg aaaattcatc tataaaagta gaaacgaagt
     1621 atggtgttat tgatataact tcacctgtag agggcgaatg tatagaattg tcagaagtaa
     1681 aggatgatgt atttgcttca aaagctatgg gagaaggtat agctgtgtta cctacaaaag
     1741 gagtaataac agcccctact gattgtgaag tagcttcttt atttccaaca ttacatgcaa
     1801 taggattaaa attagataat ggagcagaaa tgttaataca tgttggaatc aatacagtag
     1861 agttaaatgg taaatacttt acaaaacatg ttaatcaagg agatttagtg aaaaaaggtg
     1921 ataaacttat atcgtttgat atagataaaa taaaaaaggc tggttatgat gtaactacgc
     1981 cagttatagt aaacaataca tttgattttg ggcaagtagt ttcttgtaaa tctagttatg
     2041 tttcaaccaa tgataatata atttcattag tattagttgg taattaagtt aatttgacta
     2101 gagataagta aattgattta aaaggaaata agtaaatcaa caattaaata catctaaaag
     2161 taagtttatt gcaatgaatt gttaaagaaa ataattaata tggagtaaat ctattgcaat
     2221 gaagtaacaa agaaaataat taatatagag taagtttatt gtaatgaatt gttaaagaaa
     2281 agagttaata tagagtaagt ttattgtaat gaattgttaa agaaaataat taatataggg
     2341 taaatctatc tcaatgaaat aacaaagaaa agagttaata tagagtgaac ttattgcaat
     2401 gaatcaacaa agaaaataat caatataaaa aggagaaata tttatgaata caggatttcc
     2461 aaaagacttt ttatggggag catcatcttc tgcatttcaa gtggaaggag cttgggataa
     2521 agacaataaa ggaaaaaccg tagccgacta taattctttt aaaaagtctc atttacaggc
     2581 agatactaaa gtcgcatcag acttttacca taattatgaa gaagatattg aattaatgaa
     2641 agaattaggg atgaaaacat atcgtttttc aatttcatgg gcaagaataa taccagatgg
     2701 tgaaggagag ataaaccaga aaggtttaga cttttataat aagataatag acaaactaat
     2761 agagtgtgat attgagccat tcgtaacttt ataccatttt gaccttccat ttaaattagt
     2821 agagaaatat aatggttggg aaagtagaga aactgtctat gcttttgaaa gatttgctaa
     2881 aatatgcttt aagcattttg gagatagggt aaagtattgg caacctcata atgaacagaa
     2941 tttaattgtt agagtggagg agagaattaa tatatatgat gaaacagatt catggaagat
     3001 agataaaatg cgtgctcaga tggattataa tctttgctta gcacatgcat tagcagtcaa
     3061 tgcatgtcat gagatgataa aagaaagtaa aattggagca gcagtatcat catctgtaac
     3121 atatcctctt acttctaagc cagaagatgt atatgcagct agaatgaatg ataattttaa
     3181 agtgtactat atgcttgata tgcatcatta tggagaatat ccagggtatt atatgaagta
     3241 tcttgaaaaa agaaatatag ttcctcacat ggaagatggg gataaagaga tacttaaaaa
     3301 agctaaaatg gattttatag ctgtaaatta ttataggaca aattgtgcag aagctttacc
     3361 tgaagattca caacatccat ttggattaag agaaggagct gttgatttta gtatatatgg
     3421 actatttaaa atgtcaatga atcctaactt agaggcttct gagtatggag cagcaataga
     3481 cccatcaggt cttagagttg ccttaaatga atattggcaa agatatcact tacctgttat
     3541 aataacagaa aatggtctag gagctaaaga tatattagaa gatggaaaaa ttcatgatga
     3601 ctatcgtata gactatttaa gaagtcatat aaatgcatgt aaactagcta ttgaagatgg
     3661 tgtagagatg attggatatt gtccatggtc atttacagac cttttaagtt cttctcaagg
     3721 ttttaataag cgttatggac ttgtttatat aaatagaact gaccatgaaa ttttagattt
     3781 aaaaagaatc aagaaagata gtttttactg gtataaagaa gttatagaga acaatggaat
     3841 agtaaaataa acataaatat atattgaggg ttttagtatg tacattacaa aggtattaaa
     3901 taattcgttg ctacttgcaa aggatgataa tgatgaagaa ataatattga tggggaaagg
     3961 cattggccat aattataagg ccgggtctga gttaaataaa aaagatatag aaaagatttt
     4021 tgttcttcat gatgaaaata taaaaaagag tattatacaa ttggcaacag aaatagatgc
     4081 agaatatttt ggtatagctc aaatgataat tgcatatgga atagaaaaat ataacttaaa
     4141 acttatgaac catatatatt tggccttaac tgaccacatt gcctttgcag tgagacgttt
     4201 taaagaaggt ataatgattg aaaatcacta tttgttcgaa atcaaggaat ttaatccaaa
     4261 agagtatgat ataggaaaat atgctattac tgtttttaag gaggttcttg gtttagacct
     4321 accagaagaa gagattggta atatagcagg ccattttata aatgcacaac aagataatcc
     4381 ttatagtgat agaaataaga gaagtgctaa aattgtaaat gctattttgc aaatagttca
     4441 ttatcacttt tctattgtat ataataaaga gtcattttat tataggagat ttgtaatgca
     4501 tctaaaggcc ttttcacaaa gatttctggc taatgaacca tcaagagaaa aaatagattt
     4561 tatatatgag caagttcaaa aaaattgtaa ggaagagtat gaatgtgtga aaaaggtagg
     4621 agcttatatg tataaggaat actccagaga attaccaaga caagaggagt tatatttgat
     4681 gattcatatt cataagattt taggagaatt atgcgaaaat agttagaata tcttaatatg
     4741 ctagtaatat tataaaaaat taaaaattaa ctgcacttta aatatgaata attgcattac
     4801 ttaagggggt tgtcttaaaa ttgatttttt taattaaatt tgaagctcaa ctcctttttt
     4861 aaacctttat atcattacaa ctttaatgtt tatacgactg tactttagat gtatatctac
     4921 tttttacatt tctcaacatg caaacctacc attttttgca gtatgttaat tgaaataaac
     4981 ctttgagtta accctaaatt tattctggac ttataggaat agttccaaag atactttaaa
     5041 atattttctg gaataattgc ttatttcaca tatgttttat tgtgatgttt tgatttaata
     5101 aattcttcaa ctctgtatat agtaaatgta ttatcatgtt tgttttattc aagcacactt
     5161 ataatacgct tgattttttc acaatgatga aatatactaa tgggtgttat aggtataaga
     5221 atcgcattac acttgaagaa gtaagtgata aatgagatat aaatcctcat atgataaact
     5281 actatttcat tgaggaacat attctaagtg atgtcaagat actacaatat gactaaattc
     5341 ctagtgataa aaaagtctac gaatgagtgt agaaagggga actaagatga ataaagtttt
     5401 aattatagat gatgataaag atctttgtat tttgttaaaa aagagtattt caattgaaca
     5461 gattgaagca aactattgcc attctgggag agatggactg gttgaacttt caaaacaaga
     5521 ttatcagtta gtagttctag atgtgatgat gccaggtatg gatggttttg aaactatgga
     5581 acaaatacga aaatatagca gtattcccat cttaatgctg acatcaaaaa gcgataatta
     5641 cagcaaagtg catggattgc gagctggtgc agatgactat ctaaccaaac catttgaaat
     5701 tacagaattt actgctcgtg tactgtcttt aatccgacgt tacacccgtt ttaatccaaa
     5761 aaatgaggag ccacagcaac tttctttcaa aggtttgttg attgaccttg accagcatac
     5821 agttactacc aatggcgaac ctatgttgct tccacccaag gaatttggtg tacttcttta
     5881 ctgtgcaagg catcagggta agattttgac aaaacaacag atttatgaag ccgtgtgggg
     5941 tgagccatat gtatatgatg acagcaatat catggctatt atcagtcgcc taagaaaaaa
     6001 aattgaacta gacccatcat ctcccatcta tttgcaaaca ataaaaggaa ttgggtatca
     6061 attcaacacg gaggtatgaa aatggatgaa caaacgataa ttcttttctt actcttgctg
     6121 tcgattggtc tactttttct ttccatattt gctttttcca agcttgggca tatttacaag
     6181 cgcctaaaag atatagagga gatactggct gatgtacaaa ttggagacga aaatcgtaaa
     6241 atactaatta agccatgtga tgttatggct ccattggttt atcagcttaa tgagattgtc
     6301 tacgattatg aaaataagtt actttctctg aaaaagtcgg ataaagctag caaacaactt
     6361 atgacaagtt tatctcatga tgttcgtaca ccattaacaa ctctgattgg ttatctggat
     6421 gctgtacaca gtggtatagt tatcgaacaa gagcgggagg aataccttga aatcgctagg
     6481 cgtagagcct acgacctcaa ggattacatt gatgttcttt ttgactggtt tcggttgaac
     6541 tctgatgaat ttacgctgtc aatagaatct gtggaaattg cagagttgtc acgcaatatt
     6601 ttgaaagact ggattcctat attccatgaa aagaaactga attttgaaat tgatattcca
     6661 gaaaatcgac taatggtaaa cctcgaccca gatggatatt cccgtgtggt caataatctg
     6721 gttcaaaatg ttcttgctca cagtaaggct cggcaaatca agattaccat gagtgaggat
     6781 tccagaatgg tattactccg tgttgaggat gatggtgttg ggattgccag agaaaattta
     6841 ccacatattt ttgagcgact gtataagtgt gataaaggac gttcagaaaa aggaagtggt
     6901 cttggtctat ctattgtcag tcaaatggtg gagcgaatgg gtggtcaggt ttccgtagaa
     6961 agtgagattg gaaagtatac cgtatttaca gtgagcttac cattaatata aaattgtttt
     7021 tcccccatgt gacatgtata cacaggggga ttttttgtaa gctataaagt tgcaaggtta
     7081 atacaaggtt gttgcaaggt taatgtaaga ttgaggtggt atgataactt taataaacaa
     7141 aggaggcttt tattatttat gaataattta attattgaaa caaaaaatct cactaagcaa
     7201 tatggtgctc aaaagagcgt ttctaatttg aatattcacg ttcagaaagg tcgcatttac
     7261 ggattgctag gaagaaacgg tgcgggtaag acgacaacta tgaaaatgtt attgggtcta
     7321 acaaaaccaa ctactggaga aattaaaata tttggtcagg acattcacaa taacgagaag
     7381 aagcttcttc ctcgaattgg atgtttgatt gaatcaccag ggttttatcc taatctgaca
     7441 ggaacagaaa atctcaaaat ctttgcaagg cttagaggaa taccaaaaaa aaatgcagta
     7501 gaatcagcac tggatgtagt cggtttgcca aacaatgaca aaaagttatt ttctcaatac
     7561 tctcttggta tgaaacagag gttggcgatt gctcttgcca ttatgcatga cccacaactg
     7621 ttgattttgg acgagcctat caatgggctt gatcctattg gtattgcaga agtaagaaca
     7681 tttattcgtg atctctgcac agagcgaggt aaaactattt tgatttctag tcacattctt
     7741 tctgaaattg ccttgttggc agatgacatt ggcattattg accatggtat tttgttggag
     7801 gaagaaagtc ttgcccaatt agaagaaaaa aacagtaaat acatacattt tgctgtatct
     7861 gatgcagcac aggcagcaag gcttctggaa acccaactta acctgaaaaa tttcaaagta
     7921 gcagataatt acaatattat gctatatgat acagatgttt ctgttgcaga aataaatcgt
     7981 gctttcattc taaacaacat tgaggtatca gaatctcatt tatgcgaaga tactcttgaa
     8041 gattacttca agaaagtaac agggggtgaa ggaattgctt gatataattt catgtgaatt
     8101 ttcaaaactc aagcggttaa aatttatact gatttctatt ttaggtgctt gcttatttcc
     8161 aattcccgca acaattttga ttgcaaagga taacttgccc tttgagcaat tgttcaaatt
     8221 agttgtcaat tttggatatt ttttgctatt accaattgta ttaagtattg ttgctagcca
     8281 actatttttt atagaaagag ataatgatgt attaaaaaat cttgcaacag tgccagtgcc
     8341 aaaaggaaaa ctggctctgg caaaattggc tgtgttgctg tttatatcat tgttctactc
     8401 tgttgcaggg ttaggagcta ctatcattgg aggtttcatt gttggaatag tagagggggt
     8461 tgctgtaaaa ttaggaatga gcattgcttt tggaattatg ttttttgtta ctgtacttcc
     8521 tgttgtggta ctcattgtat atttcaatcg cagctatatt ttttctatta tactttcttt
     8581 tgtatatgct attttcaatt ttagtatatc cttaaatatt atcaattttg aaccaaataa
     8641 cccaatatta agtgtattac ctgctcctgt tataatgcgt tggtggatga cattttgggg
     8701 agatcccact ggcgaataca cggcacttag acagccatat ttgctctcaa ctcctgcttg
     8761 tgtaggtatt ttatgtttga taaccattat agcagtcctg ctgatttgta ttatatacaa
     8821 aaagcaggag aattaggagg tatctatgct tgatataatt actacagaat ttcaaaaaat
     8881 aaaaagatat aatattctat ggattggtgt tgttgctgtt ctgttttcag cactattggc
     8941 agtttttcag gttgtaagtt cacacggcag tgaccctcta acgtatgaga gctttgcaaa
     9001 cggagttatc tggaataatt tcagtcttag ttttccattt gggattacac ttattggagg
     9061 atatcttatt aaccgtgaat atacagacca aaccttaaaa aacatcctaa ctgttcctat
     9121 ttcattacgc aaattgctag tgggaaaatt aattgcggtt ggaggaatta ctgtattgtt
     9181 aagtcttttc agcttcttat gcacatttat tttggggttg ttattttgta atatcaatat
     9241 atctgttaca ctcgttgtaa aatccttagt tcagattttg tctgttaatc tgtattgtta
     9301 tgttgctgtt ttgccgatta ttgcttattt tggtcgtaaa caaaatgcat ttctgacggg
     9361 tgtagggatt gcatttgtat acggtttttg tggtgtgttt gttgctggaa gaaatctgac
     9421 agacttttat ccaatcacag caggtcttgg tctagttgga tacaacaatg gtgctggtac
     9481 tgtttatcag ccacttatcg gtgctatgac tttgattgta attctaatct tgacaactat
     9541 tctacttgta tttacaccta attatgataa agttatgacc atatctaaga aaaaagggat
     9601 aaagcataaa aagtattctc ataaataaca taaatattgt ttctattatt taagttgcta
     9661 gacagtgaaa agaaaaaatc tgtcgtacag tggctatatg ctcatgtatt caagaagcta
     9721 cagttttgat ttttcataac tgtagcttct ttttgtattt attaaatcac gactatcata
     9781 atacttgcaa taggtatagc gacaagagag agtactgtca tatgtgtttt atatattaca
     9841 ttaagtatat tctaaaatat atgttttaaa taatatattt gaaaatagga atatttaaat
     9901 aaaaaaaata atattatata tagttgacaa aaattggaaa atgagatact atataaatat
     9961 agaaatagta atcattacta ttattaaaaa gggaggaatt aattatgaac ttaaaaggaa
    10021 ctaaaacaga aaagaattta atggcaggat ttgcaggaga gtcagaagca agaaataaat
    10081 atacatacta cgcttcaaaa gcaagaaaag aaggttataa tcaaatagct gctatatttg
    10141 aagaaactgc taacaatgaa aaggaacatg ctaaattatg gtttaaactt ttacatgatg
    10201 gtatgccttc aacagaggaa aatttaaagg atgcagcagc aggtgaaaac tatgaatgga
    10261 ctgatatgta tgcaaaattt gctaaagaag ctagagaaga aggatttgac aagatagctt
    10321 atttatttga agctgttgga aaaatagaga aagagcatga agaaagatac ttaaagcttt
    10381 tagaaaactt aaatgaagga aaaatattta aaagagatga agaagttgtt tggcaatgtc
    10441 aaaactgtgg tcatgtgtat gtaggtactg aagcaccaga aaaatgccca gtatgtgacc
    10501 atccaaaagc atattttaat ataaaagctg aaaattatta aaaattatca ttattaaaaa
    10561 ggaatgttaa ctatgaaatt ttctaaacaa cgagaactga ttttaaatga aatattaaat
    10621 aatccggttc atcctactgc ggattaccta tatgaaaact taaaaaaaga taatccaaat
    10681 ttaagcttag gaactgtgta tagaaattta gctcagctaa cggaacatgg ctttataaga
    10741 aaggttagta ttccagggta tccagataga tttgatggtc gaatagataa tcactatcat
    10801 atcatatgtg aagtatgtgg agaggtatac gatttagaat ccgaagttct taataattta
    10861 caagaattaa tatctgagga gacagacata aaaataacat cttataacat aagttttaaa
    10921 gggatttgta ataattgtaa aaggtgtagc caagtaggtt agttttcaaa ttctaaaaat
    10981 aattaatgag gtgagtttat gtgtagtgaa caaaaatttt ttatatgtga gacttgtgga
    11041 aaccttgtag gaatgataca aagtggtggt gtacctattt tctgttgtgg aaaacctatg
    11101 aaggaacttg ttcctaatac aacagatgca gcagttgaaa aacatgtacc tgtaattgaa
    11161 gtggatggaa ataatgttac tgttaaagta agtagcacaa ctcatccaat gactaaagaa
    11221 catcatatag catgggtgta tcttatgact gaacaaggtg gtcaacgcaa atgtctagca
    11281 gtagatggag aacctgtagt taaatttgct ttgaatgatg atgataaagt gatttctgcc
    11341 tatgcttact gtaatttaca tggattatgg aaggttgaac tgtaatattt aaataaattg
    11401 agtcaaaaag gaggctataa atgtattttc atacaaatag cctcctataa atttatataa
    11461 attcaagttt taattttgtt taatgtcaat ataatataag gagaaaataa tatgtcaatt
    11521 tataaatgta gtgtttgtgg atatatttat gatgaatcta agaatgataa aacatgggat
    11581 gaattaagtg aagattggga atgccctgta tgtacaaaag gtcgtagtta ttttggaaag
    11641 ataagtactg tttactatga agaagatgaa aagatagcag aagacatagt tgaagatgaa
    11701 tctaaattaa atactgaaaa agagggagat ttaaattact taagtacata tttaagaaga
    11761 gatgatgaag ttgagaaaca tatggatata attcacgaaa tggctgttac aggaaaatct
    11821 ataatagaac ctatgagaac taaactacca gtaatttctt gggatgatat tttaattatg
    11881 ggtgctcaat taaatccatt acctttaaat gagcatgatg aagtaaatac aacaacaata
    11941 ataggaaaaa aagcaaagaa acctatgatt atagaaaatc cagtttatat ttcccacatg
    12001 tcatttggag ctttgtctaa agaactaaaa atagctttag caaagggtgc agcccaaaat
    12061 aagacagcta tgtgtagtgg tgaaggaggt atattaccag aagagaagga agcttcatac
    12121 aagtatattt ttgagtatgt accaaataaa tatagtgtaa ctgaagaaaa tcttaaaaat
    12181 tctgatgcta tagagataaa aattggtcaa ggaacaaaac ctggtatggg aggtcatctt
    12241 cctggcgaaa aagtaactga agaaatagca aaagtaagaa ataagcctgt aggtcaagat
    12301 gtaataagtc catcttgttt tgaggaaata caatccaaag aagatttaaa gaaacttgta
    12361 gatgaactta gagaagtttc agaagggcgt ccaataggtg ttaaaatttc agcaggtcat
    12421 attgaaaaag atatggaatt tatagcttat gctaaacctg attttgtaac tattgatggt
    12481 cgtggtggag ctactggagc aagtccaaag ttgcttaaag atgcaacttc aattccaaca
    12541 atatttgctc tttatcgtgc tagaaaatac atagatacac atggattaga tatagatttg
    12601 gttataacag gtgggcttcg tatatctact gattttgcta aggcaattgc tatgggggct
    12661 gatgcagttg ctattgcaag ttctgctctt atggcagcgg cgtgtcaaca atatcgtata
    12721 tgtggttctg gtaagtgtcc tgtaggagta gctactcagg atgaggaatt gagaaaaaga
    12781 ttacatatag aaaatagtgc taatagagtg gcaaactttt taaatgtaag tttagaagaa
    12841 cttaagactt ttgctagaat ttctggtcat aaagatatac atgatttaag tgttgatgat
    12901 ttatatactg ttaactctga aatatcaaat tatacaaaca tacagcatgt atagattgaa
    12961 gtttgaaact attagatttt agatagctat aaaagtaaat gataaatata ttttataaaa
    13021 gagagtgtct taaaatgaac tttttagttc ataagtcact cttttttgta tgaagccaaa
    13081 tatatttatc attttagcaa tgaaaaagaa tcttatctct ttattttgag atagactcct
    13141 tctatttatg aaaattaaag atattaaatt gtaaaatatt ctgtgatgtc gtactattaa
    13201 actaaagatt ctgtacttta aagtaaataa aaatttaagg agtgattatt atggaaagat
    13261 tgaagataca agttttagtc gacaataata cttatattga tagatacttt gttggagaac
    13321 cagcagtaag ctattatatt gagattgatg gaaataggat tttgtttgac tcagggtatt
    13381 cagatgtatt catatcaaac gctgaaaaat taaatataga tttaggaaat ttaactcatg
    13441 ttgttttttc tcatgggcat aatgaccata caagaggtat ccagttttta gaaaataggt
    13501 atgatttatc tacagttgaa cttatttcac atcctaactg ttttatacct agaaaacatg
    13561 gagagaaaag cataggagct ccattttctg ctgaggaaat taaaaatatt tttatgtata
    13621 atcctaagga taagccattt aatctaagca agaattgtgt gtttttaggt gaaatacctt
    13681 ctataaatga ttttgaaaaa agagctaaaa ttggtaaatg taaaatagga gatttgtggg
    13741 aagatgatta tgttttagat gattctgcta tagtttgtaa aactgataaa ggtattttta
    13801 ttattacagg atgttctcat agtggaattt gtaatatagt agagtatgct aaaaaggtat
    13861 gtggagatga tagagttatt ggtatactag gtggttttca tctttttgaa ttgaataata
    13921 ggttagatag tacaatacaa tatttggata aagaagaaat aagaatgttg tacccttgtc
    13981 actgtgtttc ttttaaggta aaagcgaaga tgaatgaaat attgaatata aatgaagttg
    14041 gagtagggct gatcataagt atatagtatt tgaaagtata taagatttaa gttgaaggtt
    14101 aaaaagatat tttctataga aaatatcttt tttgttacgt ttattttaag agaaatcaat
    14161 atacttttat atgaaatatt gtaaaaaaat ttatttttaa tgagaatata ttttcattaa
    14221 taaacaattt tattatgtat taataatgtt agaataaata taa
//
LOCUS       DAEORZ010000108        14105 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEORZ010000108 DAEORZ010000000
VERSION     DAEORZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.109"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            419..1312
                     /locus_tag="KRH39_002778"
     CDS             419..1312
                     /locus_tag="KRH39_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBG8512667.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1312..2706
                     /gene="gltA"
                     /locus_tag="KRH39_002779"
     CDS             1312..2706
                     /gene="gltA"
                     /locus_tag="KRH39_002779"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBG8512668.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3072..5291
                     /locus_tag="KRH39_002780"
     CDS             3072..5291
                     /locus_tag="KRH39_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8512669.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5438..6184
                     /locus_tag="KRH39_002781"
     CDS             5438..6184
                     /locus_tag="KRH39_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512670.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6184..8064
                     /locus_tag="KRH39_002782"
     CDS             6184..8064
                     /locus_tag="KRH39_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512671.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGVII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            8183..9574
                     /locus_tag="KRH39_002783"
     CDS             8183..9574
                     /locus_tag="KRH39_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8512672.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9681..10721
                     /locus_tag="KRH39_002784"
     CDS             9681..10721
                     /locus_tag="KRH39_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG8512673.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11210..11863
                     /locus_tag="KRH39_002785"
     CDS             11210..11863
                     /locus_tag="KRH39_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG8512674.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            11939..12148
                     /locus_tag="KRH39_002786"
     CDS             11939..12148
                     /locus_tag="KRH39_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512675.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12288..12854)
                     /locus_tag="KRH39_002787"
     CDS             complement(12288..12854)
                     /locus_tag="KRH39_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBG8512676.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13015..13668
                     /locus_tag="KRH39_002788"
     CDS             13015..13668
                     /locus_tag="KRH39_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBG8512677.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13826..14002)
                     /locus_tag="KRH39_002789"
     CDS             complement(13826..14002)
                     /locus_tag="KRH39_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512678.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5517 a         1400 c         2375 g         4813 t
ORIGIN      
        1 ggtattgcaa tagattaaag ccttatacac gtttagtttt taaagaaatt agtgttatta
       61 tgaaaaccta agttaattta ttaaataaat aattgaataa aaaaatttta ttatgtattt
      121 ataaaatttt tgttacaaat ttggcataat atagtatact ttaagtgtgt attaggttaa
      181 atctataaca aattagacgt atgatataga cttagtgttt attggcattt acaagaaatt
      241 tgttaatatc aactatttgt agtttgggca gttataatta ttataggggg gatgtccaat
      301 acaaaacaat caaccaagaa ctaatattat attataaaga gttatagttt tgtgtgttta
      361 tgtttttaat taaatttaaa gtgggaaaaa tatatttgtt agaatggaga atagcgagat
      421 gagtaataaa atagttagta agagacaatt aacagatagt atttatttaa tggaaataga
      481 ggcacctaga gttgcaaaat cttctcaacc aggtcaattt ataataatta aaaatgatga
      541 aaaaggagag aggattcctc ttacaatagc tgactatgat agagaaaaag gaacagtaac
      601 tattgtattc caaacagttg gtgcttcaac taaaaagtta gcaatgtttg aagaaaacga
      661 ttttgtaatg gattttgtag ggccattagg acaagcaagt gaatttatac atgaagatat
      721 agaagaatta agaaataaaa agatattatt tgtagctggt ggagttggtt ctgctccagt
      781 ttatccacaa gttaaatggt ttaaagagca tggattagat gtagatgtaa taattggtgc
      841 aagaactaaa gaattaatta tattagaaga tgatatgaaa aaagtagcta aaaatgtata
      901 tgtatcaaca gatgatggta catatggatt taatggaaga gttacagacc tattaaaaga
      961 tttagttgat aatcaaggta aaaaatacga ccaagcaata gtaattggac caatgataat
     1021 gatgaaattt atgtgccaat taactaaaga actaaatata cctactatag taagtttaaa
     1081 tacaataatg atagatggaa caggtatgtg tggtggttgt agagttagtg ttggaaatga
     1141 aactaagttt gcatgtgtag atggtcctga atttgatggt catttagttg attttgatca
     1201 agcaatgaga agacagtcta tgtacaagac tcaagaggga agagctatgc taaaacttga
     1261 agaaggcgat agtcatcatc atagtaattg tggatgtgga ggtaacaaat aatggatgct
     1321 aaaaaagtaa aagtaccagt tagagaacaa gaacctgctg tgagagcaac aaattttgat
     1381 gaagtatgct tagggtataa taaagaagaa gctatggcag aagctaatag atgtttagct
     1441 tgtaaaaaac caaaatgtgt gggtggatgt ccagtaggaa tcgacatacc agggtttata
     1501 acaaaaatta aagaagatga tatagaaggt gctgctaaag taatagctaa gagcagttca
     1561 ttaccagctg tttgtggtag agtatgccca caagaaagtc aatgtgaagg tgtatgtata
     1621 cttggaataa aatcagatgc tgtatcaata ggtaaattag aaagatttgt agcagattgg
     1681 tcaaaagaaa atgacataaa tttatctgat acagagccta agaaaaatca aaaagtagca
     1741 gtaataggaa gtggtccagc aggtctagct tgtgctggtg atttagctaa aaaaggctat
     1801 gatgtaacaa tctttgaagc aatgcatgag ccaggtggag ttttaactta cggaatacct
     1861 gagtttagac ttccaaaaca agctgttgtt caacctgaaa tagacaatat aagaaaatta
     1921 ggtgtaaaaa tagaaactaa tgtaattgtt ggtaaaacaa ttacagtaga tgaacttata
     1981 gaagatgaag gatttgaagc tatattcata ggttcaggag ctggtcttcc aatgtttatg
     2041 aatataccag gggaaaatgc aaatggagta ttttctgcaa atgagttttt aactagagta
     2101 aacttaatga aggcttatag agatgattat gatacaccta ttagttctgg taaaaaagtt
     2161 gctgtagtag gtggtggaaa tgttgctatg gacgctgcta gaactgcatt aagattaggt
     2221 tcagaatcat atatagttta tagaagaagt gaaaaagagc ttccagcaag agctgaagaa
     2281 gttcatcatg caaaagaaga aggcattata tttaatacat taacaaatcc taaggaaatt
     2341 ttagtagatg aaaatggata tgttaaaggt atggtttgta taagaatgga attaggagag
     2401 ccagatgatt ctggaagaag aagacctata gaaatagaag gttctgaatt tgtacttgat
     2461 gtagacacag ttataatgtc acttggaaca agcccaaatc cactaatatc ttctacaaca
     2521 aagagtcttg atataaataa gaagagatgc ttaataacag atgaaaatgg tcagacttct
     2581 aaagagggtg tatttgcagg tggagatgct gttactggag cagcaacagt aatatctgca
     2641 atgggtgctg gtaaaacagc agcagcatca atagatgaat atttaaaagc aaaagttaat
     2701 gcttaatttg tacattaaag agagggtgtc tcaaaatgaa ctttttagtt catgaggcac
     2761 tctttttcta caaaatcaaa tatgttttaa tcatttcaac aatgaaaatt tcattcctgt
     2821 agaaagtcta acttttaggt cttagtacaa tctctatttt gatgtagcca cttttttaga
     2881 tggtttaata atttatcagt tattattgta atatttgata ctaagtaaca taactttgtc
     2941 taactatgat aaaatagtta tacaataaag gtcttgtaag gagtgaataa tgtgtggcta
     3001 ataaaatcag atttgtcaaa tctaacatta aataatagaa aaataaattg tgtaattaat
     3061 ggtattttaa tatgaaaaaa agagaaaaag tatttgatgg gctagttttt aagcatatat
     3121 taatcttatt aatactatta gttttgattg atttaacaag taccatcata tatgcaagta
     3181 actctatgga aattacttct agaaatatga ttgaggcatc aaaaagagaa cttgaaaact
     3241 atttagaagt aaacatatct ttattaaaag ctcttagtca agatgataga ttttctaatg
     3301 gtgaaacatc actaattgaa aagggaaaat tattaagtcc atatcaaaag gaatataatc
     3361 tttttatgat tggtataaca gatacaaaag gaaatgcttc aagtacatat agggaaaatg
     3421 ttaactcaat aaaagataag cctagttttg agaaagcaat taaaacaaag caagtagtag
     3481 tttcagatat tgaagtcagt aatgtaacag gtgacaaagt atttatcata tatgtaccaa
     3541 ttgtaaaaaa taatgagatg attgggacca tttttgcttc tttttatttt caagatgtaa
     3601 ataatttaat aagtagaagc aattttgatg attctattaa atttctaatg atagataaag
     3661 attatactat tatttctcat ccaaacaaaa aatatgtaaa tgataaaagt aagatgcttg
     3721 atttagaagg gaatattata ggaaccacta aaagtgaaat cttaaaaaat ataaataaaa
     3781 aatgtcaagg tgatttttta tcttgggata attggagatt atataatgta aaatatacga
     3841 acataaagtg gactaattgg actttagtat caaaatgtaa tatatttaag aactttaaaa
     3901 atctaatagt taattttatg ataaagttgt atttttatat agtaatattt atgatattgt
     3961 ggaaattaag taatgctaaa ttaatagaac aacttaaaaa attggcttat tatgattctt
     4021 taagtggaat aaaaaataaa gaaaagttta gaaaagattc tatgtatatt ttaaagaatt
     4081 attatcaaga taatttttac ttagtacaat tggatgtcaa taaatttaaa tatataaatg
     4141 agatgtttgg atatgctgaa gggaataaga tattaattca tatatcacag gttttaaata
     4201 ataatacaaa taaatatgaa atttgtgcta ggatggataa tgaccatttt atattgttga
     4261 ttgcttgtaa cactgaagat gaacttttaa atagattatc taaaataaat aaagaaattt
     4321 gtaacttaaa tactactaac tcatctaagt ataaaattgt tatgtcaagt ggaatatata
     4381 agattactaa aaaggatgat attaaaaaga ttgacttgtt aattgataga gcaaatattg
     4441 cagcaaaaag taaaaaggaa aaatatgaac attcatattc attttttaat gaagatacaa
     4501 gaaatcgatt gtacaaggaa aaacgattgg aagataatat gaataaagct ttagaaaaag
     4561 gtgaatttat agtatattat caacctaagt atagtttaga tgatgtaaat gaaattgaag
     4621 gagcagaagc cttgataaga tggaatagtc cagaatttgg gtttatttca cctatagatt
     4681 ttgtaccttt atttgaaaag aatggtttta ttgtaaatat tgatatgttc gtgtttgaag
     4741 aagtatgcaa aacattaaac aagtggataa ataaagggta tactcctgta ccaatctctg
     4801 taaatatgtc tagagttcat ttatataggg ataactttat agaaaatata actgatttga
     4861 taagtaaata caatatatct cctgagttta ttgaacttga actaacagaa agtgttgtgt
     4921 ttgataattt aaatatattg attgatataa tgaaaaagat taaagagata ggattcctta
     4981 tatcaatgga tgattttggt tcgggatatt catctttaaa tctgttaaaa gacctttctt
     5041 ttgatatttt aaaattagat agaggatttt taatcgaaac aacagataca aaacgtggaa
     5101 aaattattat ttctaagatt gtggaaatgg caaaagcgat tgatattaaa gtaatctgtg
     5161 agggcgtaga aacttatgaa caagtagagt tcttgaaaga gataggttgt gataaggttc
     5221 aaggatactt atttgctaaa cctatggtat tagatgaatt tgaaaaacac ttaaacttta
     5281 aatttgatta atcattaata attattagac tataagatac cctaacaact tacttacaaa
     5341 tatgtaagtt aattgttagg gtatttttat ggttaaagta gcatattcac gatattatgg
     5401 tattgtagta aaaataaaaa attatggaga atataaaatg aacatattga gtattagaaa
     5461 tatatcaaaa acatattgtg gaaatattcc atttaaagca ttagataagg ttagtctaaa
     5521 tatagaaaag ggagaatttg tgtctgttat gggaccatct ggaagtggta aatctactct
     5581 tttaaatata atatctacag ttgatagaca gagtgaaggt gaggttgtat tagatggata
     5641 tgatgtaagt aaattgaaag gagaaaaact tgctgaattt agaaggaaac aacttggttt
     5701 tgtatttcag gattttaatt tgattgacac tttaactgtt ggcgaaaata ttatgttgcc
     5761 acttacacta gagggggcaa gtatcaaaga tatgaatata caaactaaaa gtatatctaa
     5821 atttttagga atagataaaa ttttagatag aaaaacctat gaaatttcag gaggtcaagc
     5881 tcaaaggtgt gctattgcta gagctattat aaataaacct gccatacttc ttgcagatga
     5941 gccaactgga aatcttgatt caaaatctac tgatgatgtt ttgaaattat ttactaggat
     6001 aaacaaagaa caaaaggtaa caacacttat ggtaactcat gaagcatata gtgcaagtta
     6061 ttcagataga gtaattttta taaaagatgg ttgtatatat acagaaataa aaaaatctga
     6121 aagcagtaat tctttctatt ctgatatact agctgttttg tctcaaatag gaggtgtaag
     6181 atagtggatt ttagaaagtt tgcttataat aatatagtta gaaatttcag ggcatattta
     6241 gcatattttt taagttctgt tttttctata atgatatttt ttgtttttgc agtgtctatg
     6301 ttccatccaa ttataacaaa atctggaatg cagagtggtt ctactgcatt tatggctctt
     6361 atgacttcag aattaataat attcggtttt agtctacttt ttatattgta ctctttagga
     6421 aatttcttaa aatatagatt gaaagatttt ggagtattga tgataattgg aatgagtaac
     6481 agacaactga aaaaattgat attaattgaa aatttgataa taggattttt agcagtgatg
     6541 ttaggaatat tacttggttt atcaatttca aaattgtttt tattgtattt aagcaaactg
     6601 ttttatatga atttaactga attttatttt ccagtaaaag caatgctatt gactattgta
     6661 tcatttatga ttttattttt gataacagga ccaatgagct taaaattatt gagtaaaaag
     6721 agcattttag agcttttagt aggtacaaaa aagcctaaaa aagagataaa atcttcaaag
     6781 ctatttggaa taattggtgt aatatgtctt atattaggat atgctatgat actatttggc
     6841 aataagttaa aaataaatgg aggtatgtat acaacagcac tattaattac tttggggata
     6901 tttttatttt tttctcaatt tactattttg atattaaagt atttaaaaaa taaagaaaaa
     6961 ttttataaga acaaagtaaa tatgttactt ataagtaatt tgatatataa attaaaggat
     7021 aatacacgat tgttattttt aataacaata cttttatcgg ctactttagt agcatttaca
     7081 acaacttcaa cacttgtaag ttctcaagga gaagcttcta aaaataattt tccaatggtt
     7141 tactcatatt tttcagagca taataatgaa aaagaatatg aagaactaca acaaattaga
     7201 gaagcaatca aaggatatga ttataaggag ttaagttttc ctgtacttaa atataatagg
     7261 gaagtcttac taagtgtaaa gaattataac aaattatcaa aggaattggg attagataaa
     7321 attagttcaa agttagaaaa atatgaaggg gttataattc ctagtagcaa ttcacaagaa
     7381 catataaatt ctttaaaaca attaaaaaat tatagtataa aaaatttaaa tatagaagta
     7441 aaaaaatcaa aaaataaaat aatttttcct acaggactgt ttagtaaagt tgtagttata
     7501 agtgatgact tatacaatga agtaaaaggt gaattttcaa aaattaattt tcatggattt
     7561 gattataaaa actggcaaag cagtgcaaag ataactgaaa atttaaaaga aaaacatttt
     7621 aatttggata gagaatataa caggtcatat tttttaacat taccagatat gtatttagta
     7681 gagttacaac aaagtaaaat tcttcaattt atgggtgttt ttataggagt gatattattt
     7741 atagcagttt ttagtttttt atacttcaga ttatatacag acgagataga agataatgta
     7801 aagtacaaaa aactatcaaa aataggtcta tcttttaaaa atatgaataa gattgttagt
     7861 atagagatag gtacattgtt ttttataccg tattttatag caattataaa tagcataatt
     7921 tctttactgt ttttaaatca agtattaaat aatagtttta agttagaaaa tttgggtata
     7981 ttactactga tatcatctat atattcactt tatttttact tattaaaaaa aatttatact
     8041 agaaaagttt ggttttttga ttaaagtggt atataataga tatataaatt aatatttaaa
     8101 aagtagttaa tattagggga gggcatttgc acctcccttg taatatgtgg aaaattgaat
     8161 attagaagaa ggaggagact tgatggaaaa tcaacaatta ttaggaacag aacgaattag
     8221 taaattacta ctaaaatatt ctataccagc tataataggt atgttggtaa atagtttata
     8281 taatgtagta gatagaattt ttattggaaa tataccaggg gttggaccac ttgcaataac
     8341 aggtcttggt gtaacaatgc caataatgac aataatactt gcatttggga tgttgatagg
     8401 tataggaact actactacca tatcaataaa acttgggcaa ggtaaagtag aagaggcaag
     8461 aaaacttata ggaaatgcaa tgacgttatc tgtaataaca ggaattatca taatgatact
     8521 tggaatatta tttgcaaata aaatacttac attgtttgga gcaagtgaaa atactttaat
     8581 atatgcaaaa tcatatatca atattatatt attaggtaca gtagtaaatc ttctttcctt
     8641 ttctttaaac cactcaataa gagctgatgg tagtcctaaa atttcagcag gaataatgat
     8701 agtaggatgt ttaactaata tagttttaga ctggatatta atatttggat tcaatttggg
     8761 aatacaagga gctgcaattg ctacagtaac ttctcaggct cttacagcta ttttaactat
     8821 agggtattat ataagtggaa aatcaaactt acgattcagt aaatctaatc taaaattaga
     8881 taaaaagcta ataaaagcag tgtttgcaat aggtatgtcg ccatttgcta tgcaacttgc
     8941 agcaagttta gttcaagtaa tatcaaatat tgcacttaaa actcatggtg gagatttagc
     9001 tataggggct atggctacta tttcttctat tgcaatggta tttttgatgc ctatctttgg
     9061 aataaatcaa ggagcgcaac ctattatagg tttcaattat ggagctgaaa aatatgatag
     9121 ggttaaaaaa gcatatttag gttcattagt agtagctact ataattttat gtatgggtat
     9181 ggtagtagtt atgcttttcc cagaagctat tataggtata tttaataaag accctgaact
     9241 tatgaatata tctgttaatg gattaagaat atacttactt atgttgccaa ttgtaggctt
     9301 atcagttaca ggaacaaact ttatacaatc tataggaaaa gcaaaaatgg ctatgttatt
     9361 gagcctttta agacaagtaa ttttattgat accagcagta ttaattcttc ctacattctt
     9421 aggcttacag ggtgtatgga cagctcaacc agtgtctgat tttatagcta cagtaattac
     9481 aggtatagtt gtatttagag aattaaaaag atatactcct aaaactgaaa aattaaatga
     9541 aaatgaaaga ctaaacgaga taactactga gtaaaaatat aaattcagat aattgagcaa
     9601 gaaattgcaa aaaatacgat tatatgataa aataaaaccg ctgtcaagga taagagaaat
     9661 tctaattatg gagtgatttt atggaattta caaagaaaag caaattaaaa aatatgtatg
     9721 taaacatgaa tgtagctaga atgttgtcta agataaatga gtataagggt agacaactgc
     9781 tttacaaaaa acaaccaaaa gaaattttag aaaacttaga aaaaaaatct ttagtagatt
     9841 gttctgaatc tacatacatt ggaaatcaaa gagatagtag taattttaat ctagaaaagt
     9901 tgataagtaa tgaagttaca cctagaagta gagaggaatt gagtattgtt gaatacagag
     9961 atgtggtaaa aactattaac agtgcttatg agagtattcc catcagttca caaacgattc
    10021 ttgagcttca tggatactta tataaatttt catctactag aggaggaagt tataagtcag
    10081 ataatgattt tattgaacac aatttaaaac ctagcgaatt tataagtatt gattcaagtg
    10141 tcaataaagt tgaaaaagct gtagaagaaa tatgtgaagc ttataataca ttgatagagg
    10201 aagatgaaat tgatatatta atattaattt ctgcatttgt gttagatttc atattgatac
    10261 atccattcaa agaaggtaat ataaaaatgg cgagagtact tatcctattg ttactaaata
    10321 aaaatgggta tgaagttgga agatatataa gtttaggaaa gatatttgat gatagttcat
    10381 atgaatatta tagtaattta aattatttga aagcctcaat aggtagtgaa aaagcagata
    10441 tgaatgcatg gattgagtat tttttagaga cgatattaac tgcatatgag aagctggatg
    10501 atagtttaaa tatatctgat aagaaaagac aaacaaaaac tagtagaata gagaaaataa
    10561 taaattctac ccttggatat tttacaaaag aagatataag agatttatgt cctgacattc
    10621 cagaacctac tataaataga gtttttaata atctaagaaa acaagataag atagaagttg
    10681 ttgcaagagg aagaagtgca aaatggaaga aaaaatatta aaaaatgttt agataactaa
    10741 aatggtatag tgttttacac tataccattt ttaatgtcat tagcatgagc aataatttaa
    10801 caacttaagc aatatcaatt ttatttattg attatttttg aactgaaaaa tggcgttata
    10861 tggtatacct cagtgataat taattttgga tgagaagata taatattaca gaatatatta
    10921 ggaatatatg tagaaaaaat ataataaaag tgtgaggctt agtaattaac aaattatgtg
    10981 gcttcacact tttttataaa ataaagatac ttagcagagt aatattttag ttgatatgtt
    11041 tataagatgg tacttgtttg ggtaaaagta taaatagtaa aaaagtagaa gtaatgtgta
    11101 taaaaacgta agttacattc tgtaataata gttattttat aattcaacaa aagaatagaa
    11161 tgggtatatt tgtgaaataa taatataaaa taatcttagg agtgagctta tgaagaaaaa
    11221 attattatat ataaatgtaa attcaaaacc tgaagactta tcttcaagta aaactgtagc
    11281 aagaaaattt ataaataaat ttatggaaaa aaataaagat tttgaggttg aagagataga
    11341 cctttataaa gaacatatac caagacttga gtatcagtac tttgaaaaaa gaaatagcat
    11401 agttagtgaa gaaaatgcta aaaatttaga cgataaggat agaaaagagc ttacaaagat
    11461 tagaaatctt tgtgaccaat ttgtgagtgc aagtgtatat gtaatagcag cacctatgtg
    11521 gagtttatct tttccagcac cacttaaaga gtatatagat tgtataattc aagatggaaa
    11581 aactatatct tttgaaggca atgataaacc acaaggaatt ttaaatgata ttgatagaag
    11641 tatggtatat atacagtctt ctggtggaca cataccatgg gtgttgaagc cagttatgaa
    11701 taaagggtta aattatgtgg aaagtattat gaaatttata ggaattaaaa aatttgatga
    11761 attgttagta gatggaactg gaactagtga agaagaaaga caagctgcaa tagaaaaagc
    11821 atctgaaaaa atagatggta taatagatgg gatgaaattt taattataat attttattaa
    11881 aaatataaag gaaaaacctc ttttaatgta gaaacttata ttgaaggagg cgagaatcat
    11941 gcgagaagaa aaaagtaatg aaaagtatga ttgttattgg tgtaatcaag agaataattt
    12001 ctgtgtagaa ataaaagata atatagtcat gatagatgat ggcactggta cgttaaaaca
    12061 agccgttttc atagggtata aacaaatcca aattaattta aattgttcac attgtcaaaa
    12121 cttaaataga ataaaattaa atttgtagaa ttttaaaatg gaaattattg aaggaattaa
    12181 aaatagaaag tgttaatatt tagactgaaa attatatttt atacaataaa agtttattta
    12241 aataacattg tattttaata tttattttca gtcttacttt attaaaatta ttttttggtt
    12301 atgtctggaa aatatctgtc tactaatttt tctgaaatat tttttaattg gtcagccatt
    12361 gaagtcagac ttaataaggc tatcaataaa tcatctttat tttctggatt tatactaata
    12421 ttttcaaaat tagactctat aaagtccggt atattctttc tttgattttc ttttattaac
    12481 atgaattttt cgtaacatga aactaaatct ttttcactga aattatcctt ttcatacaca
    12541 ccatgtaaga ttcttttaat ttcttgtatt gtaagtgtat ttttcataga ataaataatt
    12601 atcatctgta aaatttgttc tttagtatac ttttttccct taactggttt gattaatccc
    12661 tctttgctgt aattatttat catggtttta gtaagaattt tatcactttc aaatctttta
    12721 ttagcagaaa gcttattgtc aattagagaa ataatttgat ccatatataa gtcaatagat
    12781 ggaatatcgt ttgaagttat atctgcatta tttagtgttt ctagtattat agtattaagt
    12841 tcattattca tcatatgtta cctccataat atatataata cactaaatta gaaagtaaaa
    12901 caatattaag gcttgtaaaa aaaacataaa gtggtataat taaatatata atatagtaat
    12961 ggaaactata tttgtgaatc tgatttttaa aatacataat tgtaggagga cgaaatgtat
    13021 caattttttt taaagggtag agacccaata agcagtttaa ctcattttat aggtgcttgt
    13081 ttatctcttt tagcaactat aattttagtt tttcaatctg ttacattaca ggaaacttct
    13141 ctcttgatga tagtatctgt atctgtattt ggactttcac taattgcttt atatagtgca
    13201 agttcgtatt atcatttttt aaaggggaca ccagaacaag aattgttttt tagaaaagta
    13261 gaccatgcaa tgatttacgt attgattgct ggctcttata caccaatatg tctgaatttt
    13321 atggaaaaaa aagaaggcat aatatttgtt acagcaatat ggatagttgc atttattggc
    13381 ataataataa aaatattttg gatggatgca cctagatggt tatctaccag tatatattta
    13441 cttatggggt gggcaattgt ttttgatata aatgcattca attcaattcc taaggattgc
    13501 cttagactat taattatgga aggtgtatct tactctatag gtgcgattat ttatataatt
    13561 aaaaagccta atataagtcc tgagtttgga tttcatgaaa tattccatat ttttattatg
    13621 ataggttctt tatttcactt tttagcagtt ttattgtatg ttttatagtt ttaaaagatt
    13681 tgattaattg atactaaaat tctacaatga ataaaatgga actgccttat atagattata
    13741 tcttcgacaa taagacagtt ccttattata aatattaaat tctacttata ataaaatttt
    13801 gataaaaata gtccattcaa aatgcctatt ccataattcc tttaatgtac ttttcattta
    13861 cgacatatag tttgaattta tgtactataa ttatatcctc taaattctct gaaacactaa
    13921 caattctgac acatttgttt ctaatttcta caggtaaatc taaatcattt gttaaattca
    13981 catagtcatc atatttatac ataaaatcat ccctttacat taagatatac ttattatact
    14041 atattttaat aagttttttt aaattattac aatttttccc gaaatttcat tttttataca
    14101 taaaa
//
LOCUS       DAEORZ010000109        14092 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEORZ010000109 DAEORZ010000000
VERSION     DAEORZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14092
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.110"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(106..948)
                     /locus_tag="KRH39_002790"
     CDS             complement(106..948)
                     /locus_tag="KRH39_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8512679.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(1200..2984)
                     /gene="pepF"
                     /locus_tag="KRH39_002791"
     CDS             complement(1200..2984)
                     /gene="pepF"
                     /locus_tag="KRH39_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG8512680.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3050..3715)
                     /locus_tag="KRH39_002792"
     CDS             complement(3050..3715)
                     /locus_tag="KRH39_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG8512681.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(3866..4027)
                     /locus_tag="KRH39_002793"
     CDS             complement(3866..4027)
                     /locus_tag="KRH39_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBG8512682.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4105..4614)
                     /locus_tag="KRH39_002794"
     CDS             complement(4105..4614)
                     /locus_tag="KRH39_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBG8512683.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            4820..5038
                     /locus_tag="KRH39_002795"
     CDS             4820..5038
                     /locus_tag="KRH39_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG8512684.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5056..7002
                     /gene="feoB"
                     /locus_tag="KRH39_002796"
     CDS             5056..7002
                     /gene="feoB"
                     /locus_tag="KRH39_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG8512685.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(7181..8236)
                     /locus_tag="KRH39_002797"
     CDS             complement(7181..8236)
                     /locus_tag="KRH39_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG8512686.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(8284..9084)
                     /locus_tag="KRH39_002798"
     CDS             complement(8284..9084)
                     /locus_tag="KRH39_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG8512687.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9077..9868)
                     /locus_tag="KRH39_002799"
     CDS             complement(9077..9868)
                     /locus_tag="KRH39_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8512688.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(9908..10330)
                     /locus_tag="KRH39_002800"
     CDS             complement(9908..10330)
                     /locus_tag="KRH39_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBG8512689.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(10346..10825)
                     /locus_tag="KRH39_002801"
     CDS             complement(10346..10825)
                     /locus_tag="KRH39_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8512690.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSNLIK"
     gene            complement(10957..13713)
                     /locus_tag="KRH39_002802"
     CDS             complement(10957..13713)
                     /locus_tag="KRH39_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8512691.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         4570 a         2431 c         1607 g         5484 t
ORIGIN      
        1 tatatttcta cattcaataa tagacaatac cctgcaagtt aaagcatcat ttttatgagc
       61 ttatctcaca gggtattata tgtttacttt atattataac tcttgttatt gattaacttt
      121 aataaatcct ttttctgaaa caagtgcttg tccttcatca cttaatatat aatctattaa
      181 agctttacct ttttctgaca aaccatcttc tttattaact aatataaatg gtcttgatat
      241 tggatattta ttagctatta catttgcatc agttgcttca acatcattta tttttaaact
      301 atttactttc tcatcaacat atccaaatga tatatatcct atagcatttt catttccttc
      361 aactgtagtt tttatatttc ctgaaccatc acttatttgt gcatctttag ttagttcttc
      421 tgaatcaaac cctacaattt cttggaatcc atctctagtt cctgaacctt cttctcttga
      481 tactattact attggtgcat catttcctcc tacttctttc cagttagtaa tctttcctgt
      541 atatatacct ttaacttggt ccattgtcag attttttact gagtttgcag tattagttat
      601 caatgctata ccatctattg ctattttagt ttcctttaat ccagcacttt tttcttcatc
      661 ttttaaatct cttgaactca ttcctatttc agatgttcct tctatagcat tttttatacc
      721 tccagaagaa ccaatctgtt gtacttctac actaacacct tgattttttt cttgaaactt
      781 ttctccgata gtttccataa gaggacctat tgaagttgaa cctgaaacag aaactttgct
      841 tgaatctcct gaatcacctg aacctcctga gttagaacta catcctactg ctaaagctcc
      901 aactaacatt gtactagcta aaagaacgcc tattttcttt ttaaacattt tacttacccc
      961 cgcttatatt ttaatttttt gttgtcattt ttctatattt taataataca gattctttgt
     1021 taaatgaata ttagagcagg gttaagtaaa tgtaaatatt gttaaataca tgtaaaaact
     1081 taattttata tgatttattc ttaattagat taacttttta aagttacttt aggcagatat
     1141 aaacataata caactcatat tatcagaagt cttcattgaa gctatcaacc tatcacatcc
     1201 tataattctt tttctagttt attaactaat tcagcaaata catttaaggc ttcctctata
     1261 gattcttttt tcgtcatatc tacaccagca gattttaatt gttctagtgg gtattctgac
     1321 cctccacttt ttaagaagtt tatatatcta tctactgcag tacttccttc tgaaagtatc
     1381 tgttgactta aagcacttgc agcagaaaac cctgtggcat acttatatac atagaagttt
     1441 gaataaaaat gaggtattct cgcccattct attcctattt gtttatctac tattgcagat
     1501 tttccgtaat acttttcatt taatttgtag tatatattag taaattcatc agcagtaagt
     1561 ggttctccac tctctaccct ttgatgtaca attttttcaa attcagcaaa catagtttgt
     1621 ctatgaactg tagttctaaa ttgttctaaa taataattta ataagtatat agtttcatcc
     1681 ttagacttag aattttctaa taaataattt ataagaagta attcatttaa tgtagatgca
     1741 acttctgcaa cgaatatctt atagtccgaa tataaaaatg gttgacttgt tcttgaataa
     1801 tggctgtgaa cagagtgccc taattcatga attaaagtga atagagaatt aaggtcattt
     1861 ttatagctca ttaatatata aggatgtgaa tcataacttc cccaagaata agctcctcct
     1921 tttttacctt cattatcata tacatcaatc cacccttcat caaaagcact ttttatgacc
     1981 ttcaagtatt cttctcctaa aggtttaagt gcctctaata tgatatcctg tgctttatca
     2041 tatggtatat ccatgtcaaa tttatctgtc aaaggaacat acaaatcata catgtgtatt
     2101 tcattcaatc ctaaaaattt ctttttaagc tcaacatatt tatttagatt aggaatactt
     2161 ttatctatag ctgatataag attattgtaa acatctaaac ttacatcatc tgaaaataaa
     2221 gacgcttcta ttgctgaatt atactttcta gtttttgcgt aaaatatctc tgattttatt
     2281 ccaccataaa gcgttgaggc aaatgtattt ttatatttct catatgttga aaattcacct
     2341 tcaaaagcgt cttttctaac tctactattc ttacttttta taaataaaga gtagtttgaa
     2401 tgagaaagct taaccttatt cccctcctca tcttctatct caggaaattc catatctgca
     2461 tatgctaaca tatcgtatac attttctgga attgtcgata attctgatgt agctgcaagt
     2521 atttcttcct ctcgttcact caaagtatgt ggtttatctc tcaatatatc ctttatatat
     2581 tttgtataaa aggatagctt ctcatctttt aagtattcac ttaacttatt ctcttctatc
     2641 gccagtattt ctggaactat ataagaagta gccacaccta aatcagtaga taacatttct
     2701 gtcttagtgg ctcttccttg attatcatta attctagtat cttcatgttg tttcatatga
     2761 gtatatacat ataaattttg aacaatccta gatgcatttt cagagatatt taatacttta
     2821 tataagttct ccttactctc agaaagcttc cctttatatt cttttacttc ttggataaga
     2881 ttttttactt tacttatgtc tttttcaatt tcctctttac tggagtacat cttatctatc
     2941 ttccatttgt acttttcttc tattgtactt ctatcagttc ccatatcata cacctctatt
     3001 ttaatttgtt ttatatttta ttcactttac aaaatatatt gtatatattt tagtccaaat
     3061 aatctacatt tattactcct tctatcatat ttatttcttt cataacgtcg tgtaagtgta
     3121 ttctcttagc ataagataca aaaaaattaa ccttccagta ttcttcatta cgacattcta
     3181 tttccatatt tcttacgata agttgatttt tttcaaattt atcatataaa aaaggtatag
     3241 agtccaaatt gcttaatttt aatatcaata cattcttttc ttggctaaat aaaaacgtat
     3301 tgattatcct aagcacaaat aatgctatca ttacaaataa tccacctaga attgccaatt
     3361 cataataacc atatccaact gctataccaa tacatgcagt tgcccacatc ccagcagctg
     3421 tagtaagccc ttttactcca cctttatttt ttaaaattgc tcctgcacct aaaaaaccaa
     3481 ttccagataa tacttgagca ggcattcttg ctgggtcagc atttgtatac aaattataat
     3541 caaagaataa ttgaatcgaa gttattgaaa caatacaaga acctattgca attagtatat
     3601 gtgtcctaaa tcctgcaaac tgtcttattt tttctctctc taagcctatt attcctccta
     3661 ttattagtgc taatagtaac cttattgcta tctccttact tcctatatca atcataatat
     3721 aaaaccctcc tgacttaatt acaacttatg tcctacagat aatttacaca tcagaaatac
     3781 tattttatat cttaatatta ttattaatat atgtaaaata tagccatctg ttaatttttg
     3841 atacaaaaaa acccacaact gtgggctatt tgcttaggta catgtattta tttattaata
     3901 tatctatttt cttacttgat tctactaact cttcttgaaa tatttctgga tattctatgt
     3961 atgtatttat ctcttcccta actgcttcaa tctcattttt taacgcttct aattcattgt
     4021 tcgccataat aatcaccact attacaaatt tgtatatttt tatatacatt ttatcaaaca
     4081 atattatatt ttgtcaatat tatgtcaata aaacttacta taaaaatgct ttaatttagg
     4141 tattgtatac ctggatacta agcctacaaa cataccagtt actaatgatg ccattaacat
     4201 aaatggtaag taccctatag caagtatatt ttctatgatt aaacttgcaa ctattaattg
     4261 acctatatta tgccctattg cacctataat gcttatccct attagtgata aatcttttat
     4321 tttactcgct aaatacataa ataaaagact cagataagct cctcctatac taaatagtag
     4381 atatgataaa gggcctgcaa atattgatga caaaattacc ctgatagtaa caattataac
     4441 agtatctttg acacctaaca acattaatga aattaaagtt ataatattgc tcaaacccaa
     4501 ttttgcccct ggaaatataa ctataagagg atttggtata tatgtctcta atacatataa
     4561 agctaaacta taccctatca taagacctaa aaatgtcatt tttctagttt tcattaagtt
     4621 aactcctcca aattttatct atcatcttac attataatac aaaatatata aaaacaccta
     4681 atttagacta aaatattttt aatctattta gaataaatat tagttatttc aatgtgcaat
     4741 tacctctata aatcttgact ttttgtatca tacttgttac aattttaatg ataatgaatt
     4801 tcattaggag gtgttaataa tgactgttta cgatttaaaa ataggagaaa aaggatttat
     4861 taatagtata gatggagata ttaaattatc taaaagattg cttgcacttg gatgtataaa
     4921 tagtactgaa atagaagtta aaaaggttgc cccacttgga gacccaatta taattagatt
     4981 tagaggcttt gaccttgcga ttagaaaatc tgatgctaaa aatatttctt tacaataata
     5041 gaggaggagc tacccgtgat taatgtagca ttattaggaa acccaaatgt tggtaaaact
     5101 actgttttta atctacttac aggttcaaat caatatgtag gtaactggcc tggtgttact
     5161 atagaaaaaa aagaaggatt tcttggaaaa gagataaagg ttgtcgattt acctggtata
     5221 tatgcaatgg atactttctc aaatgaagaa aaggtttcta aatcatattt agaaaatgaa
     5281 gatgttgatg taatagtaaa tgtagttgat gcttctaatt tatcaagaaa cctttactta
     5341 acaactcaac ttatgcaatt taacaagcct atagttatat tgcttaatat gttagacatt
     5401 gcagaatcta aaggagttaa tatagatgcc aaaaaattaa gtgaagaact tggagtaatt
     5461 gttgttccaa ttatagcaaa aaagaaaaat ggtgttgata ggatagaaga agagattaag
     5521 ggagctacgc aaaaagcttt tatatatcag aaaaactttg gaagtgaaac agaaacatat
     5581 aaaaaattag aatccatttt atcaagatgt acaaaaacta catatacaca aaagaaatct
     5641 atcagtgata agatagacaa tatagtcttg aatcctatat tagcatatcc aatatttatt
     5701 ggtgtattgt tcctcttatt taaatttacc tttgactggg tcggaggtcc actacaagag
     5761 ggatttgcta gtcttataga ggcttatatt tctgcacctg ttagtgatat gttatctaac
     5821 tctagcccat ggtttaaatc acttatagtt gatggaataa ttggaggtgt tggtggtact
     5881 ttaccattct tccctttaat atttacatta ttctttggta tatcgttgtt tgaagatagt
     5941 ggatacatgt caagaacagc atttttaatg gataaagtta tgagaaaggt tggtctatct
     6001 ggtaaagctt ttatacctat agttatgggt atgggatgtt cttctccagc tataatggca
     6061 actagaacat tggaaagcga aaaagataga agagttacag ctcttattgc accacttatg
     6121 acttgtggag caaagttacc tatatatgca ctatttgtag ctatattctt tccacataat
     6181 gctgctttag taactacatc actgtatttg gtgggtatag ttatggcaat tgtagttgca
     6241 ttattcttaa ataaaacagc ctttaaaaca gaggctgaac catttatatt agaattacca
     6301 gaatatagaa tccctactat agatgcactt attaaaaata cttggaataa atcaaaaggt
     6361 ttcttgataa gagttgttac tgtcatgttt gcaatgtcag tcgtaatttg gggactttct
     6421 tcattcaatg tttttggata tacagaagat ataaatgtaa gcttcttggc tactctaggt
     6481 catattatat ctcctatatt caaacctctt ggttttgatg attggagaac ttcagttgct
     6541 attttaagtg gtcttggagc aaaagaaatc gtagtaaact ctttaaatat actatatggt
     6601 aatctaactg ttgtcttacc aacaatattt aatggagtta ctgcatatac attcttgata
     6661 tttactgctt tatatactcc atgtatagct gcacttgcaa ctatgaaaaa agaatatggc
     6721 aataaaatga tgtttacatc atttgcatat caatttatac tagcatggat aatggctttt
     6781 atagttaaaa gtataggtgg agtactattt atgggtaatt caataataga agctttgata
     6841 ggtggaataa ttatagtcct tgcactcttt attctgttta ataaattcaa gtctacaaaa
     6901 aacggaaatg tctgtagtgg atgttctatt tgttctggat gtccaagtgc taattcttgc
     6961 tcaagtaagg aagacagcaa taaaaaaaat aatgcacact aatatataaa tattttagtt
     7021 taaaataaga taaaagtggg tgtctcaaaa ttaacttttt agttcatgag gcactctttt
     7081 ttatagaaaa tcaaatatat tttcatcatt tcaacagtaa aaaagagctt atctagtaaa
     7141 aaagaggctt atctctattt tgagacaacc tctttttttg ttacttattc agtttcttgc
     7201 tacctaactt catgtggaac ctagtatctt ttggaacact tgggtctaca cctcttaatg
     7261 taggtatttc cccacatatt agttgtatag gtactataaa attaaatgtt tgatagtaag
     7321 gattatctgt gatatcaatc tttaaatttt tatcattttc tgtaacatct ctacctatag
     7381 caaatacatt ttccgtccaa ccagaaagta catcaatcat cttagttact ctaggttctt
     7441 ttcctgtatc tagtataaat atagtagaat cactatttat agcattatat ataccatgta
     7501 taaactcttc aaactcatac ccagttacag gtattctcat tgtttccaat aattttaatg
     7561 ctgcttctaa tgtatctcca tatatatcag agtgacctat tattcttatt tctttagaat
     7621 taactaactt ttctttgttt ctttcaatcc attgtttaga gagcttatac actgcttcaa
     7681 atctattaat tgcgtctaat atcttattta tttcctcatt gtacttttca ctagatatta
     7741 tacctttttc tctagctatt tgaagaccta ataacattaa gtttaacttt gtacaataat
     7801 atccttttgt tttagctcca gatttttctt caccacaatt tactgttaat atataatcag
     7861 aaatctcatc tattaaagca ttcttacaac ctgccataga tgctatttta cagcccttat
     7921 cttcagctaa cttcatagcg ttgtatgtag aataactact cccaccttga gatacaccta
     7981 ctactagggt attttcatta tcaaacttga atgtatcttc agttatcata aatggataca
     8041 ttttcacaac atctatatct aataaatttt gtaaatatgg ttgcacttgc actcctgagt
     8101 gatagctagt acctgaacct gttattataa tcttttttag atttgttctc ttaacttcat
     8161 taaataagct atctgcatta gatataattt ctctcattct aactggtgtt tctaacatat
     8221 aatcttgtat tgtcatttta tacaccttcc ttatctatat attcaataaa ttataattaa
     8281 tttctataac aaacctatag catgtaaagc gattcctgtt actatagtgc ctatagttaa
     8341 ttttgttaca cttacctttc tctttaacat gtagtatatt acaaatgcaa aggttaaagg
     8401 taacatatta ggtattattt tatctagtat atcttgtaaa attacttctg caccatttaa
     8461 gttgaatttc agaggagtag ttatactcaa catagttgca accattcctc ctacaacgaa
     8521 taaaccaaca gttgtagcca tagacattat tttctccata agtccctctc tttgtattct
     8581 ttcaagagaa tttaccccta cttgataacc atacttaagt ccaaatattc taagggcaaa
     8641 tgctggtata ttgtataata gtaagaataa tagtggtcca aagatattac cttcttttgc
     8701 aagtgatact cctactcctg ctgctataat cctaaatgtt ccccagaata atgaatcacc
     8761 aattcctgca agaggtccca taagagctgt tttaacagca ctaattgaac tttcatcaaa
     8821 ttctggatta ttagcattct cttcttccat agctgctgtt atacctaata tagcaggaac
     8881 tactactgtc gttgtattaa aaatctccaa atgcctttct attgcttttg cttgattttc
     8941 tttcttattg tatagcttct ttattgcagg taacattgaa taacagtaac ctaagttttg
     9001 cattctttcg taattaaatg caccttgaag agcaaatgaa cgccaaaaca tacttcttaa
     9061 ttcttttttt gttaatttat tcaaagtcaa tctctccttc cgttaaagta ttgattgcta
     9121 attgaggatt ttgattattt tctttgttat tgatataaaa gtttagtaca aatgctacac
     9181 atactccaaa taaagctata gcagttatat ctaatccacc ataagatgct aacaaaaacc
     9241 ctataaagaa aaatacagac atcttttttg aaagtaacat atccataagc aatgcaaaac
     9301 ctactgctgg caacaatgtt cctgcaacac ccagtccgtc taatataact ttaggtattg
     9361 catttatcaa tgctgtaact ttatcagcac caatcataat tgctaaaaag gttggaactg
     9421 atgtacttaa aaagaataat attactggca accacatcat tatggtaact ttcttaaaat
     9481 cagcctgttt agcatagaaa actgcctttt gagaaaagta cgaatttatt attctaacta
     9541 atactccaag agattgtgct aagactgcta ctggtactgc tactgctaaa gcaacttctg
     9601 caccattacc tgataatata gcaaaagctg tacctaatac acctcccgtt actacatctg
     9661 gaggagtagc tgcacctatt cctgcaattc ccatccaaat taattcaagc tgtgcaccaa
     9721 tcataattcc tgattttatg tctcctaaaa ctaatcctac taacatacct gtaactattg
     9781 gcctatcaag catagattga ccaaatatac gcccatctag tataccaact cctgctataa
     9841 tagctaacat agtagcttgt actaacatat tgaacctcca ttttaactta gtttaattta
     9901 cattaaatta cattacattt ttgattttgt tttgattttc taataactca tttacataaa
     9961 caattccatt ttttgcttcc tctaatcctg atttaactat taattttgta tcctcctcaa
    10021 ctaataaaag tatagataaa agcattggta aatttactcc tgtcaataat tcaaatttga
    10081 acccttgtaa aagtaagttt gtacatatgt ttgccacact tccaccaaaa acatctgtta
    10141 agacaacaaa ttcttcacta tctttattat ttgataatat acttctaatt tctttctcag
    10201 catcatctgc tgatttatcc gttgtcatac aaaaatactc aatattagtt aatgaaccaa
    10261 ttattaattt acttgtgtca acaagctctc tggacaattc tccatgtgtt gcaattagaa
    10321 attttttcat tctctttctc ctaaattatt ttattaaatt tgatagttgt ttctttttgt
    10381 ctctaggtaa cattctaaat tctatatcaa tccctaaatc acctatttgt ttaaagttat
    10441 ttatatcatc atcatcaaca gcaactaaat ctgtaagttt cttcttccct tcctcatatc
    10501 ttaatcctcc tacatttatt tcttttaata ctcctcctga atttttagct acaactaaag
    10561 catctttggt attttttact aacactaata cattgctttt ctttgaagaa gcaaattttt
    10621 ctactatttc tccagattcc tctacccccc ttatatataa agttacattg ctaggtttcg
    10681 ctaagttcag tgacatagct ttcatttggt cattttttgc ttcatcattt gcaactagta
    10741 ttacatttat acctagtgat gttgcccatc catatgcaac ctgaccatgt atcaatctgt
    10801 catcaattct catcaaagat accattttcc ccaaatcctc catcatacat taaaattttt
    10861 attgtttata ctctcattta tctataaaag caaaaagtat gccaaatact ggcatacttt
    10921 tttaaatttc attcacttat tttgtaatct tgtattttac tgtggtagac catagataat
    10981 attataaata tatcctatct ctgaaattgg tatttgcaca ctataactct tttctagcac
    11041 actaaatgca ttttttatta gttttacttc actatgatgg tttttgatta acaaatccat
    11101 atcagtatac tcttcaattt cagtttgtct aactaatctt tcaatcatac aacttaagtg
    11161 aacatataaa gaaatctttt tatcatttga aagttttttc ttttgaatat tttctatagc
    11221 attaattcct tcttctattc tctctattat cttttttgaa tctaaaatag ttagactatc
    11281 tattactcta attaatgaaa aattctttac aatagaatca tttatgttct ttattttttc
    11341 attttctact actctgctaa atattctaaa aacatcatct tctccttttc ctgatattat
    11401 atcctctaat aatataaaat ctaccccatt cacatttgga ttggcagttc ctactatagc
    11461 catcacatca tacatttcaa aaactggttc tttatctccc atagaaacaa gactatcata
    11521 atcataaggt attattttga catcaactat tccaattaaa ctttcggaaa gtagatgttg
    11581 gatttttata gcagtaccta tccctgtaaa acaagatgat attatagcat attctttatt
    11641 tttttgagga taatacaatt gtatattagg tttagtttct tcatccataa tttctgcaat
    11701 ctcttcaatg tttttacctt gcattaataa atttcctact tctagtgcag aaatagtgga
    11761 tatttggtct ataaataaca ttgggctatt tatcttattc tttaaatctt catatataag
    11821 attaagagag cccatatcaa ataatacaat taaaccattc tttatcctat ctccctctag
    11881 gtatctagac atttgttttg atacatcagc aatagtagca tctataggca tatcaatagc
    11941 tgtaaatata tttttaccca acatccgatt acatacattc gccatactac tggcagtagc
    12001 atatccatgt gctataatag tcgcattaac ttgattaaat ttcattctta taattgttga
    12061 tttaataaat ataatcatta taattgtatc tacattatta agatacatat ccaatctatt
    12121 cgttattaat gttgatattt tacttattat agagtattct ttgtaactct ctttttttaa
    12181 atatattagt aatttatttt ctaaagcttt atactcatca gacaattgat aagattcgtc
    12241 cttcaaataa agataagacg aaaatgccaa tacattattc ccatcatatt ttatattgtt
    12301 attaacttct aaatttctaa atatatcctg cattatcgat attataagct ccataagtac
    12361 gttattattt tcatcattat ttgaaaagac taaattgtcc attagtctaa tcattagatt
    12421 agaactcttt ttactaaatt tttcctttgt tatttcattt ttttctagtc tatcaaagta
    12481 atctaaacat tttatgaaac tttgatagat tatatttctt ttatttcttc catttgacat
    12541 ttcaatacta aattgtttag gagaaaagat aatgcttttg cttttaccac tttggaattg
    12601 aaatatatct acattttcta tataaagatt tctaggaagg tcttgaagag taacaaaaat
    12661 atttccattt cttctttgat tttttgcata agctcgtcca catgcatatt ttattacatt
    12721 tttacattca cctatattcc ccttgaattt tatctttaaa atcgcattta atgtaatttg
    12781 agttatctct ataggcaaac caagtacctt actttcttca taaataaaat gttctacaat
    12841 ctttcttttt tcaatagtcc ctctttcttc tatattggga acataaatat ttatgggtat
    12901 tcttcttaaa aaggtttgta aaaattcaga tagactttct gttgtagcaa atattaatct
    12961 taccctagct tttctaatta ctccattctc tccaatccta gaaaacgtac ctgtatccat
    13021 aaatgtaaat aacttttcct gaccttcact atttaatcta tgtacttcat ctaaaaataa
    13081 aacaccacca tctgattgtt ctattagacc tgctctagat tcatttgctc cagtatatgc
    13141 tccttttaca tgcccaaata atagactaga caataactcc aaattattat aatactgcgc
    13201 acagtttaac acattgtatg gagaattttt ttctaataca ttactgctta cactatattt
    13261 gtatattatc ttggctaaaa aagtttttcc tactccactt tcacctgtca gcattattgt
    13321 aagtccgcca tttggataaa gtacagcggt ttttatttgt tcgattactt cttttaagct
    13381 attatcatat cctatgatac tattaaacac agtctcttgc tttaataatt tacactcatt
    13441 tttaagttca tctaaatttt cataaatact tttttttgta ggccctacta catcatttat
    13501 tttattttta ggtaaaaaaa taacaggtct actgtttatc tttataagat tatcagattt
    13561 tactaaatca tttaataatc tacttgattg agttctactt aaattaaact tttttactat
    13621 atcttctgtt attatgccac tttctttttt aagttcatca aagttgttaa ataatacaca
    13681 ttcatttaca aaacttaaaa tttcatccct catgttcaac ctccaataca aatatcaata
    13741 atactataat attagtatat catttatata tttatgaaaa agataattat attcctatct
    13801 tgattctctt ttgtaaggtt gtaaaatcaa actttttata agtataaaat ctagattttt
    13861 taacataatc tcaaggttct tatctttcca tatatctgac atagaattta tataagaata
    13921 tatttattgt atttattata ataaaaagta aaacattata ggtattttaa aacttattag
    13981 cgaagaaaat atttgtaact tttattacct tatgaatatt atttcaaatt taaaaaggct
    14041 ataaataaat ttatttttaa ataaatctac ttataacccc acaataatac tt
//
LOCUS       DAEORZ010000110        13932 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEORZ010000110 DAEORZ010000000
VERSION     DAEORZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13932
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.111"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            201..7301
                     /gene="tcdB"
                     /locus_tag="KRH39_002803"
                     /gene_synonym="toxB"
     CDS             201..7301
                     /gene="tcdB"
                     /locus_tag="KRH39_002803"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBG8512692.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKVSELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGSTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDDKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            7423..7923
                     /gene="tcdE"
                     /locus_tag="KRH39_002804"
     CDS             7423..7923
                     /gene="tcdE"
                     /locus_tag="KRH39_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBG8512693.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8002..8163
                     /locus_tag="KRH39_002805"
     CDS             8002..8163
                     /locus_tag="KRH39_002805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512694.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYMDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            8252..8434
                     /locus_tag="KRH39_002806"
     CDS             8252..8434
                     /locus_tag="KRH39_002806"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512695.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKKIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            8651..>13932
                     /gene="tcdA"
                     /locus_tag="KRH39_002807"
                     /gene_synonym="toxA"
     CDS             8651..>13932
                     /gene="tcdA"
                     /locus_tag="KRH39_002807"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021414120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG8512696.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKIL"
BASE COUNT         5622 a         1470 c         2220 g         4620 t
ORIGIN      
        1 ttacatattt atttcagaca acgtctttat tcaatcgaag agcaaattaa tcaactgagt
       61 gtcttcaatt taaaatgtta ggaagtgaat gtatatgaaa acctaagtag atattagtat
      121 attttataaa tagaaaggag gatatataaa agagttttag catttagatg taaaaatatt
      181 atagtaaagg agaaaatttt atgagtttag ttaatagaaa acagttagaa aaaatggcaa
      241 atgtaagatt tcgtactcaa gaagatgaat atgttgcaat attggatgct ttagaagaat
      301 atcataatat gtcagagaat actgtagtcg aaaaatattt aaaattaaaa gatataaata
      361 gtttaacaga tatttatata gatacatata aaaaatctgg tagaaataaa gccttaaaaa
      421 aatttaagga atatctagtt acagaagtat tagagctaaa gaataataat ttaactccag
      481 ttgagaaaaa tttacatttt gtttggattg gaggtcaaat aaatgacact gctattaatt
      541 atataaatca atggaaagat gtaaatagtg attataatgt taatgttttt tatgatagta
      601 atgcattttt gataaacaca ttgaaaaaaa ctgtagtaga atcagcaata aatgatacac
      661 ttgaatcatt tagagaaaac ttaaatgacc ctagatttga ctataataaa ttcttcagaa
      721 aacgtatgga aataatttat gataaacaga aaaatttcat aaactactat aaagctcaaa
      781 gagaagaaaa tcctgaactt ataattgatg atattgtaaa gacatatctt tcaaatgagt
      841 attcaaagga gatagatgaa cttaatacct atattgaaga atccttaaat aaaattacac
      901 agaatagtgg aaatgatgtt agaaactttg aagaatttaa aaatggagag tcattcaact
      961 tatatgaaca agagttggta gaaaggtgga atttagctgc tgcttctgac atattaagaa
     1021 tatctgcatt aaaagaaatt ggtggtatgt atttagatgt tgatatgtta ccaggaatac
     1081 aaccagactt atttgagtct atagagaaac ctagttcagt aacagtggat ttttgggaaa
     1141 tgacaaagtt agaagctata atgaaataca aagaatatat accagaatat acctcagaac
     1201 attttgacat gttagacgaa gaagttcaaa gtagttttga atctgttcta gcttctaagt
     1261 cagataaatc agaaatattc tcatcacttg gtgatatgga ggcatcacca ctagaagtta
     1321 aaattgcatt taatagtaag ggtattataa atcaagggct aatttctgtg aaagactcat
     1381 attgtagcaa tttaatagta aaacaaatcg agaatagata taaaatattg aataatagtt
     1441 taaatccagc tattagcgag gataatgatt ttaatactac aacgaatacc tttattgata
     1501 gtataatggc tgaagctaat gcagataatg gtagatttat gatggaacta ggaaagtatt
     1561 taagagttgg tttcttccca gatgttaaaa ctactattaa cttaagtggc cctgaagcat
     1621 atgcggcagc ttatcaagat ttattaatgt ttaaagaagg cagtatgaat atccatttga
     1681 tagaagctga tttaagaaac tttgaaatct ctaaaactaa tatttctcaa tcaactgaac
     1741 aagaaatggc tagcttatgg tcatttgacg atgcaagagc taaagctcaa tttgaagaat
     1801 ataaaaggaa ttattttgaa ggttctcttg gtgaagatga taatcttgat ttttctcaaa
     1861 atatagtagt tgacaaggag tatcttttag aaaaaatatc ttcattagca agaagttcag
     1921 agagaggata tatacactat attgttcagt tacaaggaga taaaattagt tatgaagcag
     1981 catgtaactt atttgcaaag actccttatg atagtgtact atttcagaaa aatatagaag
     2041 attcagaaat tgcatattat tataatcctg gagatggtga aatacaagaa atagacaagt
     2101 ataaaattcc aagtataatt tctgatagac ctaagattaa attaacattt attggtcatg
     2161 gtaaagatga atttaatact gatatatttg caggttttga tgtagattca ttatccacag
     2221 aaatagaagc agcaatagat ttagctaaag aggatatttc tcctaagtca atagaaataa
     2281 atttattagg atgtaatatg tttagctact ctatcaacgt agaggagact tatcctggaa
     2341 aattattact taaagttaaa gataaagtat cagaattaat gccatctata agtcaagact
     2401 ctattatagt aagtgcaaat caatatgaag ttagaataaa tagtgaagga agaagagaat
     2461 tattagacca ttctggtgaa tggataaaca aagaagaaag tattataaag gatatttcat
     2521 caaaagaata tatatcattt aatcctaaag aaaataaaat tacagtaaaa tctaaaaatt
     2581 tacctgagct atctacatta ttacaagaaa ttagaaataa ttctaattca agtgatattg
     2641 aactagaaga aaaagtaatg ttaacagaat gtgagataaa tgttatttca aatatagata
     2701 cgcaaattgt tgaggaaagg attgaagaag ctaagaattt aacttctgac tctattaatt
     2761 atataaaaga tgaatttaaa ctaatagaat ctatttctga tgcactatgt gacttaaaac
     2821 aacagaatga attagaagat tctcatttta tatcttttga ggacatatca gagactgatg
     2881 agggatttag tataagattt attaataaag aaactggaga atctatattt gtagaaactg
     2941 aaaaaacaat attctctgaa tatgctaatc atataactga agagatttct aagataaaag
     3001 gtactatatt tgatactgta aatggtaagt tagtaaaaaa agtaaattta gatactacac
     3061 acgaagtaaa tactttaaat gctgcatttt ttatacaatc attaatagaa tataatagtt
     3121 ctaaagaatc tcttagtaat ttaagtgtag caatgaaagt ccaagtttac gctcaattat
     3181 ttagtactgg tttaaatact attacagatg cagccaaagt tgttgaatta gtatcaactg
     3241 cattagatga aactatagac ttacttccta cattatctga aggattacct ataattgcaa
     3301 ctattataga tggtgtaagt ttaggtgcag caatcaaaga gctaagtgaa acgagtgacc
     3361 cattattaag acaagaaata gaagctaaga taggtataat ggcagtaaat ttaacaacag
     3421 ctacaactgc aatcattact tcatctttgg ggatagctag tggatttagt atacttttag
     3481 ttcctttagc aggaatttca gcaggtatac caagcttagt aaacaatgaa cttgtacttc
     3541 gagataaggc aacaaaggtt gtagattatt ttaaacatgt ttcattagtt gaaactgaag
     3601 gagtatttac tttattagat gataaaataa tgatgccaca agatgattta gtgatatcag
     3661 aaatagattt taataataat tcaatagttt taggtaaatg tgaaatctgg agaatggaag
     3721 gtggttcagg tcatactgta actgatgata tagatcactt cttttcagca ccatcaataa
     3781 catatagaga gccacactta tctatatatg atgtattgga agtacaaaaa gaagaacttg
     3841 atttgtcaaa agatttaatg gtattaccta atgctccaaa tagagtattt gcttgggaaa
     3901 caggatggac accaggttta agaagcttag aaaatgatgg cacaaaactg ttagaccgta
     3961 taagagataa ctatgaaggt gagttttatt ggagatattt tgcttttata gctgatgctt
     4021 taataacaac attaaaacca agatatgaag atactaatat aagaataaat ttagatagta
     4081 atactagaag ttttatagtt ccaataataa ctacagaata tataagagaa aaattatcat
     4141 attctttcta tggttcagga ggaacttatg cattgtctct ttctcaatat aatatgggta
     4201 taaatataga attaagtgaa agtgatgttt ggattataga tgttgataat gttgtgagag
     4261 atgtaactat agaatctgat aaaattaaaa aaggtgattt aatagaaggt attttatcta
     4321 cactaagtat tgaagagaat aaaattatct taaatagcca tgagattaat ttttctggtg
     4381 aggtaaatgg aagtaatgga tttgtttctt taacattttc aattttagaa ggaataaatg
     4441 caattataga agttgattta ttatctaaat catataaatt acttatttct ggcgaattaa
     4501 aaatattgat gttaaattca aatcatattc aacagaaaat agattatata ggattcaata
     4561 gcgaattaca gaaaaatata ccatatagct ttgtagatag tgaaggaaaa gagaatggtt
     4621 ttattaatgg ttcaacaaaa gaaggtttat ttgtatctga attacctgat gtagttctta
     4681 taagtaaggt ttatatggat gatagtaagc cttcatttgg atattatagt aataatttga
     4741 aagatgtcaa agttataact aaagataatg ttaatatatt aacaggttat tatcttaagg
     4801 atgatataaa aatctctctt tctttgactc tacaagatga aaaaactata aagttaaata
     4861 gtgtgcattt agatgaaagt ggagtagctg agattttgaa gttcatgaat agaaaaggta
     4921 gtacaaatac ttcagattct ttaatgagct ttttagaaag tatgaatata aaaagtattt
     4981 tcgttaattt cttacaatct aatattaagt ttatattaga tgctaatttt ataataagtg
     5041 gtactacttc tattggccaa tttgagttta tttgtgatga aaatgataat atacaaccat
     5101 atttcattaa gtttaataca ctagaaacta attatacttt atatgtagga aatagacaaa
     5161 atatgatagt ggaaccaaat tatgatttag atgattctgg agatatatct tcaactgtta
     5221 tcaatttctc tcaaaagtat ctttatggaa tagacagttg tgttaataaa gttgtaattt
     5281 caccaaatat ttatacagat gaaataaata taacgcctgt atatgaaaca aataatactt
     5341 atccagaagt tattgtatta gatgcaaatt atataaatga aaaaataaat gttaatatca
     5401 atgatctatc tatacgatat gtatggagta atgatggtaa tgattttatt cttatgtcaa
     5461 ctagtgaaga aaataaggtg tcacaagtta aaataagatt cgttaatgtt tttaaagata
     5521 agactttggc aaataagcta tcttttaact ttagtgataa acaagatgta cctgtaagtg
     5581 aaataatctt atcatttaca ccttcatatt atgaggatgg attgattggc tatgatttgg
     5641 gtctagtttc tttatataat gagaaatttt atattaataa ctttggaatg atggtatctg
     5701 gattaatata tattaatgat tcattatatt attttaaacc accagtaaat aatttgataa
     5761 ctggatttgt gactgtaggc gatgataaat actactttaa tccaattaat ggtggagctg
     5821 cttcaattgg agagacaata attgatgaca aaaattatta tttcaaccaa agtggagtgt
     5881 tacaaacagg tgtatttagt acagaagatg gatttaaata ttttgcccca gctaatacac
     5941 ttgatgaaaa cctagaagga gaagcaattg attttactgg aaaattaatt attgacgaaa
     6001 atatttatta ttttgatgat aattatagag gagctgtaga atggaaagaa ttagatggtg
     6061 aaatgcacta ttttagccca gaaacaggta aagcttttaa aggtctaaat caaataggtg
     6121 atgataaata ctatttcaat tctgatggag ttatgcaaaa aggatttgtt agtataaatg
     6181 ataataaaca ctattttgat gattctggtg ttatgaaagt aggttacact gaaatagatg
     6241 gcaagcattt ttactttgct gaaaacggag aaatgcaaat aggagtattt aatacagaag
     6301 atggatttaa atattttgct catcataatg aagatttagg aaatgaagaa ggtgaagaaa
     6361 tctcatattc tggtatatta aatttcaata ataaaattta ctattttgat gattcattta
     6421 cagctgtagt tggatggaaa gatttagagg atggttcaaa gtattatttt gatgaagata
     6481 cagcagaagc atatataggt ttgtcattaa taaatgatgg tcaatattat tttaatgatg
     6541 atggaattat gcaagttgga tttgtcacta taaatgataa agtcttctac ttctctgact
     6601 ctggaattat agaatctgga gtacaaaaca tagatgacaa ttatttctat atagatgata
     6661 atggtatagt tcaaattggt gtatttgata cttcagatgg atataaatat tttgcacctg
     6721 ctaatactgt aaatgataat atttacggac aagcagttga atatagtggt ttagttagag
     6781 ttggtgaaga tgtatattat tttggagaaa catatacaat tgagactgga tggatatatg
     6841 atatggaaaa tgaaagtgat aaatattatt tcaatccaga aactaaaaaa gcatgcaaag
     6901 gtattaattt aattgatgat ataaaatatt attttgatga gaagggcata atgagaacgg
     6961 gtcttatatc atttgaaaat aataattatt actttaatga gaatggtgaa atgcaatttg
     7021 gttatataaa tatagaagat aagatgttct attttggtga agatggtgtc atgcagattg
     7081 gagtatttaa tacaccagat ggatttaaat actttgcaca tcaaaatact ttggatgaga
     7141 attttgaggg agaatcaata aactatactg gttggttaga tttagatgaa aagagatatt
     7201 attttacaga tgaatatatt gcagcaactg gttcagttat tattgatggt gaggagtatt
     7261 attttgatcc tgatacagct caattagtga ttagtgaata gataaaaata tgttaaatat
     7321 atcctcttat acttaaatat ataaaaataa acaaaatgat acactacata aagtgttcta
     7381 tctaatatga agatttacca ataaaaaggt ggactatgat gaatgcacag tagttcacct
     7441 ttttatattt ctaatggtaa caaaatattt ttttatataa acctaggagg cgttatgaat
     7501 atgacaatat cttttttatc agagcatata tttataaagt tagtaatttt aactatatca
     7561 tttgatacat tattaggatg tttaagtgca ataaaaagtc gtaaatttaa ttctagtttt
     7621 ggaatagatg gaggaatcag aaaagtagca atgatagcat gtatattttt tttatcagta
     7681 gttgacattc ttacaaagtt taacttttta tttatgttac cacaagattg tatcaatttt
     7741 ttaagactaa aacatcttgg aatatctgaa tttttctcta ttttatttat tttatatgaa
     7801 agtgtaagta tattaaaaaa tatgtgctta tgtggattac cagtacctaa gagattaaag
     7861 gaaaaaatag caattttact agatgcaatg acagatgaaa tgaatgctaa ggatgaaaag
     7921 taagtaatgg tagatataat aaagatatta acaaataaaa agtgttatcc aaataagaat
     7981 agctgaaagt tatcataatt catgaaacta ataatgaaaa cgagggagca gatgccaaga
     8041 gacacacaag tattaaatac atataatttc gaagcaagtg ttcattacta tatggatgac
     8101 aaggtagtat atcaaacatt ggttcacaaa gatggtgcat ggtcagttgg taaaatctat
     8161 taagctacat tagttacaga tatcacaaac tataatagtt aaacatagaa atatgtgtaa
     8221 attgtgatgg aaattattca aaaacacaaa aatacgtgat gaaggacaaa atgatataga
     8281 aaataagtat caaaccttaa taaatgattt aattgatagt ttaaaagtta taggaaaaat
     8341 atataaaaaa ataaaaacat taaaaaaata taagatatgt ttacaaatta ctatcagaca
     8401 atctccttat ctaatagaag agtcaattaa ctaattgagt atctttaaat tgaaatgtta
     8461 ggaagtgatt taaatatgaa aacttaaatt ataaaaaatc aatattaatt tatttttaaa
     8521 aaatagaaag gagtgtataa gatttatttt caaagtttaa aaacaagaaa atcaatttaa
     8581 atttcagaag gaataaatgt ggttatagaa gtggatttat tatcaaaaat aataatacta
     8641 ggaggttttt atgtctttga tatctaaaga agagttaata aaactcgcat atagcattag
     8701 accaagagaa aatgagtata aaactatact aactaattta gacgaatata ataagttaac
     8761 tacaaacaat aatgaaaata aatatttaca attaaaaaaa ctaaatgaat caattgatgt
     8821 ttttatgaat aaatataaaa cttcaagcag aaatagagca ctctctaatc taaaaaaaga
     8881 tatattaaaa gaagtaattc ttattaaaaa ttccaataca agccctgtag aaaaaaattt
     8941 acattttgta tggataggtg gagaagtcag tgatattgct cttgaataca taaaacaatg
     9001 ggctgatatt aatgcagaat ataatattaa actgtggtat gatagtgaag cattcttagt
     9061 caatacacta aaaaaggcta tagttgaatc ttctaccact gaagcattac agctactaga
     9121 ggaagagatt caaaatcctc aatttgataa tatgaaattt tacaaaaaaa ggatggaatt
     9181 tatatatgat agacaaaaaa ggtttataaa ttattataaa tctcaaatca ataaacctac
     9241 agtacctaca atagatgata ttataaagtc tcatctagta tctgaatata atagagatga
     9301 aactgtatta gaatcatata gaacaaattc tttgagaaaa ataaatagta atcatgggat
     9361 agatatcagg gctaatagtt tgtttacaga acaagagtta ttaaatattt atagtcagga
     9421 gttgttaaat cgtggaaatt tagctgcagc atctgacata gtaagattat tagccctaaa
     9481 aaattttggc ggagtatatt tagatgttga tatgcttcca ggtattcact ctgatttatt
     9541 taaaacaata tctagaccta gctctattgg actagaccgt tgggaaatga taaaattaga
     9601 ggctattatg aagtataaaa aatatataaa taattataca tcagaaaact ttgataaact
     9661 tgatcaacaa ttaaaagata attttaaact cattatagaa agtaaaagtg aaaaatctga
     9721 gatattttct aaattagaaa atttaaatgt atctgatctt gaaattaaaa tagctttcgc
     9781 tttaggcagt gttataaatc aagccttgat atcaaaacaa ggttcatatc ttactaacct
     9841 agtaatagaa caagtaaaaa atagatatca atttttaaac caacacctta acccagccat
     9901 agagtctgat aataacttca cagatactac taaaattttt catgattcat tatttaattc
     9961 agctaccgca gaaaactcta tgtttttaac aaaaatagca ccatacttac aagtaggttt
    10021 tatgccagaa gctcgctcca caataagttt aagtggtcca ggagcttatg catcagctta
    10081 ctatgatttc ataaatttac aagaaaatac tatagaaaaa actttaaaag catcagattt
    10141 aatagaattt aaattcccag aaaataatct atctcaattg acagaacaag aaataaatag
    10201 tctatggagc tttgatcaag caagtgcaaa atatcaattt gagaaatatg taagagatta
    10261 tactggtgga tctctttctg aagacaatgg ggtagacttt aataaaaata ctgccctcga
    10321 caaaaactat ttattaaata ataaaattcc atcaaacaat gtagaagaag ctggaagtaa
    10381 aaattatgtt cattatatca tacagttaca aggagatgat ataagttatg aagcaacatg
    10441 caatttattt tctaaaaatc ctaaaaatag tattattata caacgaaata tgaatgaaag
    10501 tgcaaaaagc tactttttaa gtgatgatgg agaatctatt ttagaattaa ataaatatag
    10561 gatacctgaa agattaaaaa ataaggaaaa agtaaaagta acctttattg gacatggtaa
    10621 agatgaattc aacacaagcg aatttgctag attaagtgta gattcacttt ccaatgagat
    10681 aagttcattt ttagatacca taaaattaga tatatcacct aaaaatgtag aagtaaactt
    10741 acttggatgt aatatgttta gttatgattt taatgttgaa gaaacttatc ctgggaagtt
    10801 gctattaagt attatggaca aaattacttc cactttacct gatgtaaata aaaattctat
    10861 tactatagga gcaaatcaat atgaagtaag aattaatagt gagggaagaa aagaacttct
    10921 ggctcactca ggtaaatgga taaataaaga agaagctatt atgagcgatt tatctagtaa
    10981 agaatacatt ttttttgatt ctatagataa taagctaaaa gcaaagtcca agaatattcc
    11041 aggattagca tcaatatcag aagatataaa aacattatta cttgatgcaa gtgttagtcc
    11101 tgatacaaaa tttattttaa ataatcttaa gcttaatatt gaatcttcta ttggtgatta
    11161 catttattat gaaaaattag agcctgttaa aaatataatt cacaattcta tagatgattt
    11221 aatagatgag ttcaatctac ttgaaaatgt atctgatgaa ttatatgaat taaaaaaatt
    11281 aaataatcta gatgagaagt atttaatatc ttttgaagat atctcaaaaa ataattcaac
    11341 ttactctgta agatttatta acaaaagtaa tggtgagtca gtttatgtag aaacagaaaa
    11401 agaaattttt tcaaaatata gcgaacatat tacaaaagaa ataagtacta taaagaatag
    11461 tataattaca gatgttaatg gtaatttatt ggataatata cagttagatc atacttctca
    11521 agttaataca ttaaacgcag cattctttat tcaatcatta atagattata gtagcaataa
    11581 agatgtactg aatgatttaa gtacctcagt taaggttcaa ctttatgctc aactatttag
    11641 tacaggttta aatactatat atgactctat ccaattagta aatttaatat caaatgcagt
    11701 aaatgatact ataaatgtac tacctacaat aacagagggg atacctattg tatctactat
    11761 attagacgga ataaacttag gtgcagcaat taaggaatta ctagacgaac atgacccatt
    11821 actaaaaaaa gaattagaag ctaaggtggg tgttttagca ataaatatgt cattatctat
    11881 agctgcaact gtagcttcaa ttgttggaat aggtgctgaa gttactattt tcttattacc
    11941 tatagctggt atatctgcag gaataccttc attagttaat aatgaattaa tattgcatga
    12001 taaggcaact tcagtggtaa actattttaa tcatttgtct gaatctaaaa aatatggtcc
    12061 tcttaaaaca gaagatgata aaattttagt tcctattgat gatttagtaa tatcagaaat
    12121 agattttaat aataattcga taaaactagg aacatgtaat atattagcaa tggagggggg
    12181 atcaggacac acagtgactg gtaatataga tcactttttt tcatctccat ctataagttc
    12241 tcatattcct tcattatcaa tttattctgc aataggtata gaaacagaaa atctagattt
    12301 ttcaaaaaaa ataatgatgt tacctaatgc tccttcaaga gtgttttggt gggaaactgg
    12361 agcagttcca ggtttaagat cattggaaaa tgatggaact agattacttg attcaataag
    12421 agatttatac ccaggcaaat tttactggag attctatgct tttttcgatt atgcaataac
    12481 tacattaaaa ccagtttatg aagacactaa tattaaaatt aaactagata aagatactag
    12541 aaacttcata atgccaacta taactactaa cgaaattaga aacaaattat cttattcatt
    12601 tgatggagca ggaggaactt actctttatt attatcttca tatccaatat caacgaatat
    12661 aaatttatct aaagatgatt tatggatatt taatattgat aatgaagtaa gagaaatatc
    12721 tatagaaaat ggtactatta aaaaaggaaa gttaataaaa gatgttttaa gtaaaattga
    12781 tataaataaa aataaactta ttataggcaa tcaaacaata gatttttcag gcgatataga
    12841 taataaagat agatatatat tcttgacttg tgagttagat gataaaatta gtttaataat
    12901 agaaataaat cttgttgcaa aatcttatag tttgttattg tctggggata aaaattattt
    12961 gatatccaat ttatctaata ctattgagaa aatcaatact ttaggcctag atagtaaaaa
    13021 tatagcgtac aattacactg atgaatctaa taataaatat tttggagcta tatctaaaac
    13081 aagtcaaaaa agcataatac attataaaaa agacagtaaa aatatattag aattttataa
    13141 tgacagtaca ttagaattta acagtaaaga ttttattgct gaagatataa atgtatttat
    13201 gaaagatgat attaatacta taacaggaaa atactatgtt gataataata ctgataaaag
    13261 tatagatttc tctatttctt tagttagtaa aaatcaagta aaagtaaatg gattatattt
    13321 aaatgaatcc gtatactcat cttaccttga ttttgtgaaa aattcagatg gacaccataa
    13381 tacttctaat tttatgaatt tatttttgga caatataagt ttctggaaat tgtttgggtt
    13441 tgaaaatata aattttgtaa tcgataaata ctttaccctt gttggtaaaa ctaatcttgg
    13501 atatgtagaa tttatttgtg acaataataa aaatatagat atatattttg gtgaatggaa
    13561 aacatcgtca tctaaaagca ctatatttag cggaaatggt agaaatgttg tagtagagcc
    13621 tatatataat cctgatacgg gtgaagatat atctacttca ctagattttt cctatgaacc
    13681 tctctatgga atagatagat atatcaataa agtattgata gcacctgatt tatatacaag
    13741 tttaataaat attaatacca attattattc aaatgagtac taccctgaga ttatagttct
    13801 taacccaaat acattccaca aaaaagtaaa tataaattta gatagttctt cttttgagta
    13861 taaatggtct acagaaggaa gtgactttat tttagttaga tacttagaag aaagtaataa
    13921 aaaaatatta ca
//
LOCUS       DAEORZ010000111        13883 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEORZ010000111 DAEORZ010000000
VERSION     DAEORZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13883
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.112"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            179..1195
                     /gene="gap"
                     /locus_tag="KRH39_002808"
     CDS             179..1195
                     /gene="gap"
                     /locus_tag="KRH39_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG8512697.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            1590..2759
                     /locus_tag="KRH39_002809"
     CDS             1590..2759
                     /locus_tag="KRH39_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512698.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYSPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            3139..7392
                     /locus_tag="KRH39_002810"
     CDS             3139..7392
                     /locus_tag="KRH39_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG8512699.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKSEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEVIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDIPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            7541..8035
                     /locus_tag="KRH39_002811"
     CDS             7541..8035
                     /locus_tag="KRH39_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG8512700.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GTDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            8151..8528
                     /locus_tag="KRH39_002812"
     CDS             8151..8528
                     /locus_tag="KRH39_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBG8512701.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            8734..9174
                     /locus_tag="KRH39_002813"
     CDS             8734..9174
                     /locus_tag="KRH39_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBG8512702.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
     gene            9819..11126
                     /locus_tag="KRH39_002814"
     CDS             9819..11126
                     /locus_tag="KRH39_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512703.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLNEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    11212..11474
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11600..12403
                     /locus_tag="KRH39_002815"
     CDS             11600..12403
                     /locus_tag="KRH39_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8512704.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            12441..13052
                     /locus_tag="KRH39_002816"
     CDS             12441..13052
                     /locus_tag="KRH39_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG8512705.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            13084..13755
                     /locus_tag="KRH39_002817"
     CDS             13084..13755
                     /locus_tag="KRH39_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG8512706.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         5611 a         1381 c         2483 g         4408 t
ORIGIN      
        1 cactaaaaag tacatactaa atgaggggta aaataaagaa aaatgatata ctaattgtct
       61 tttgataata aaaaaacatg gaatttgatt atttaatcaa gaaaaagtat tgaacaaatg
      121 atttatttag tgtacaataa tcatgtaaaa acatagtaat ggagggagac atttcataat
      181 gaaaattaag gtaggaatga acggatttgg aagaatagga agagctgtat taagaatagc
      241 tcaagaagaa ttaggagata atatagaaat tgtagccata aatgcaagag caacaacaga
      301 atctttggca catttattta catatgattc atgctatgga acttttagag gtgaagtaga
      361 ggcaaaggat gaagacactc tagtagtaaa ctctaaagag ataaaaatat taagatataa
      421 tgacccagaa gaattacctt ggaaagaatt gggggttgat atagtaatag aatcaacagg
      481 attatttaca caaagagaaa aggcagaaaa acatattaaa gcaggagcaa aaaaagtaat
      541 tataacagct ccaggaaaaa atgaagatat aactatagtt atcggagtta atgagaaaga
      601 atatgataat gaaaagcaca atataatttc aaatgcatct tgtactacaa attgtctagc
      661 tccttttgct aaagtattgg acgaaaaatt tggtatagta aaagggttaa tgactacagt
      721 acattcatat actaacgatc aaagaatatt agataaaagc cataaagatt tgagaagagc
      781 aagagcagcg gctgaatcta taatacctac cactacagga gcagcaaaag ctgtgtcaag
      841 agtactacca caattagaag gaaaattaaa tggtttttca cttagagtac caacaccaac
      901 agtttcactt gttgatttag tgtgcgaact taaagaaaac gttacatctg agcaagttaa
      961 ctctgtgtta aaagatgctg ctgaaggaga attaaaaggt gttttaggat attgtgacaa
     1021 gccacttgtt tctatagatt ataaaggaga ttcaagatca tctattatag acgctctatc
     1081 cacaatggtt atagaagaca atatggttaa ggtagtttct tggtatgata atgaatgggg
     1141 atattcttca agaactgttg acttagtaaa atatgtagct gaaaaattaa aataatatat
     1201 atttcttaaa gcaaatttta aaaaaatttt ttaaaaaaat tatataaaac taaaaaaaaa
     1261 gattatgaaa aatattgaag gaaaaatttt ttcataatct tttttctttc atgcaaaaag
     1321 atacaagtta caacttatat ttgcatatta ggtcgttaga gaagtatata tatatttagt
     1381 aaggaatcat actttttata aaatgaaaga taagatgtcg aaaacttgca taaatctgaa
     1441 aaatttttat tattaaaata ctgatttcca ttttaaaaat gataaaaaaa tgacaaataa
     1501 agttgcaaat agtaatactt ataagtataa ttaaaaaaga gaaaaggttt tgttaaactt
     1561 tataacaaaa aaagaaggga tgattcaaaa tggagaagtt aacagacagg agacaggtta
     1621 aggcggtaag catggaaacc ttcattttcc taatattact agtagttggt tttgggtatg
     1681 ttggtagtat tatgggagca ggaatgatgt tcaaagtaat aatgagtaca gcacatgctt
     1741 tactattgga gacagttttc ttaattatgg ctatggcagt tcttgcaggc gcattaagcg
     1801 cactactttc agagtttgga gtaatagcgt taattaataa aattttcgct gtatttatga
     1861 agcctttata tggactacct ggtgctagta tagcaggagc tataacaact tatttatcag
     1921 ataatccagc tattatacct tttgctaaag ataaaacatt cacacagtat tttaaacaat
     1981 accaagttcc agcattatgt aacttaggaa cagcctttgg tatgggactt atcttaacaa
     2041 catttatgat ttctcaaggt acagaatatg tacttccagc attaattggt aatttaggtg
     2101 caattatagg tagtgtcata agtgttagaa taatgttgac atttactaag aagttttata
     2161 aatacagtcc ggaagaagat aaagcaacag gaacattaga gaaaaaggat gaatttagag
     2221 aaatcagaga aggaaatgta tttcaaagag ctttagatgc aattcttgaa ggtggtaaaa
     2281 tgggggtaga tatgggaatg gctataattc ctggagtcct agtagtatgt acattggtga
     2341 tgttattaac ttttggacca tctacagacc ctgttacagg tcaagaagtt tatacaggag
     2401 ctgcatatga aggaataaaa ttacttccag ttattggaga taaacttgga tttatattag
     2461 aaccattatt tggatttaca tcaccagagg caatagcatt ccctataact tcattgggag
     2521 ctgttggcgc agcaatgtct ctagttccag agtttataaa gtcaggagct ataactccta
     2581 atgatatagc agtatttaca gctatgggta tgtgttggag tggatattta agtacacata
     2641 ttggtatgat ggatgcatta aatgctagac agttggcagg taaagctatt ctttctcata
     2701 caataggtgg tttatgtgca ggagcagctg ctcactttat atttacactc gtaggataaa
     2761 tgtaatcagc taagataaaa aataatatac aaaaaatggt tgttgatatg taataagaat
     2821 ttacatatca acaaccattt ttttctgact aaagataatt tataatacat aaacattaag
     2881 tggatattag agttaagtta tatttatgta aaaagtaaca aaaatctgta gaaatttatg
     2941 acaaaattta gtacactaac tatatgttct tatttaaaat ttaaggttag aattaggaat
     3001 tacctagtag agtaagcttt gattaataaa agaatcataa aataaaaata ttagtcataa
     3061 agattataaa taacttactt aaaataaagc catagaactt acaaaaatta aaaaagcata
     3121 taaatatgga ggggtacaat ggatgaatta tttcatatag gaattgatgt aggttctaca
     3181 acggtgaagg ttgtggtact taataatagt aataatatag ttcataaaga atatagaaga
     3241 cattattctg atgtaaaaaa atcagttaaa gaagtactaa atggtatata tgaaaaattg
     3301 ggagatataa atactaccat tataataact ggttcaggtg gaattggaat atcaaaaaaa
     3361 ttaggtgtaa aatttgttca ggaagttata tctagtacaa aatcaataga gtattttcat
     3421 cctgaaactg atgttgtcat agaacttggg ggtgaagatg caaagataac atatctaagt
     3481 ggaggaatag accaaagaat gaatggaata tgtgcaggtg gcactggagc attcatagac
     3541 cagatggcat cacttttaaa gacagatgcc agtggattaa atgaacttgc taaagggtat
     3601 aatgttatat atcccatagc ttcaagatgt ggtgtttttg ctaaaacaga tattcaacct
     3661 cttataaatg atggagccaa tcagactgat atagcaatga gtattttcaa tgcagttgtc
     3721 gtacaaactg tgagtgtcct ttcttgtgga agaaaaatag aaggaaatgt tgcgtttttg
     3781 ggtggtccat tgtacttttt atctgaactt agagaagcat ttaaaagagt attagattta
     3841 aaagatgaaa acataatctt cccagaaaat gcacaacttt atattgctat tggagcagga
     3901 ttattatcag tagatgaaga taatatacaa ctaaaatctc taatagaaaa attggatagt
     3961 ataaagaata ttgaagatgg agaagtaaat ttattagaac ctttatttaa agataaaagt
     4021 gagtatgaag aattttcaag aagacatgaa aaagaaaaaa taaattatgt ggacataaat
     4081 tcaataaaaa gcaattgtta tttaggaatt gatgcaggct caactacaac taaagcagca
     4141 cttattgatg aggatggaag acttgtatat tcttattata atagcaatga aggaaatcca
     4201 ttaaaaacaa ccattaaagt tataaatgaa gtatatgata tattaccaaa aaatattaaa
     4261 atattgagtt caactgtaac tggatatgga gaaggtctta ttaaaaaagc tcttaaaatc
     4321 gataacggag agatagagac tatagcacat tacaaagctg ctaaattttt caataaagat
     4381 gtagatttta tacttgatat tggtggtcaa gatatgaagt gcctaaaaat aaaagatgga
     4441 gtaattgata atataatatt aaatgaggct tgttcatcag gttgtggctc atttttagag
     4501 acatttgcaa gttctttatc tatgacaata gaagaatttg cacacgaagg tatttattct
     4561 caaaatcctg tagatttagg ttcaagatgt actgtattta tgaattctag agtaaaacaa
     4621 gctcaaaaag aaggtgcaag tgttggagat atatcagcag ggctatctta ttcagtaata
     4681 aaaaatgctt tatttaaagt aataaagatt agagatttaa atgagattgg taataatatt
     4741 gttgtacaag gtggaacctt ttataatgat ttggtgctta gaagctttga aaaactaatt
     4801 ggaaaaaatg taattagacc taatatttca ggaattatgg gagcttttgg ttcagcactt
     4861 atagctaaag aaaaatatga aagtggatac aaaacatcct tgttatctaa ggaagagttg
     4921 aataatatca agctagaagc aagtgtaact agatgtaaag gttgttcaaa tcattgtctt
     4981 cttacaataa ataaattttc tgataatgaa atttttattt caggtaatag atgtgagaag
     5041 ggtgaagtta tttatggtga taaaaaaata ggattagaaa agaaaaaacc aataaattta
     5101 tttaagtaca aatacaatag aatttttaga tacaaaccat tggaaaaaga agaagcaaaa
     5161 aatggggaaa tagggatacc aagagtattg aatatgtatg aagattatcc attttggttt
     5221 acttttttca atgctatggg ctttagagtt gtactttcag ataggtcatc aaaacagtta
     5281 tatgaaactg gtataactag tattgcatct gaaacagttt gttatccagg caaattagtt
     5341 catggacata tagaaaattt aattgcaaaa ggtataaaaa atatatttta tccaagtgta
     5401 acaaatgaaa ataaagagga ttcaaatgca gataattatt ataattgtcc tgttgtaata
     5461 tcatattctg aagtaataaa aaacaatgta gagaatataa gaaataagaa cataaattat
     5521 ataaatccat ttataagtct aaatgataag gaaaaattga agaaaagact atatgatgaa
     5581 ttatcaagac atttttctga tataaaaata actaaagaag aagttaatca tgcagttgat
     5641 aaagctactg atgaacagaa tttatttaaa atggagatac aagcagcagg tgaaagagca
     5701 cttaaagaaa taaaagaaag aaatatgaag gggattgtgt tatgtggaag accatatcat
     5761 atagacccag aaataaatca tggaatgcca gagcttataa attcattaga tatggcagtg
     5821 cttactgaag actctatatg tcatttagcc aatgtacaaa gaccacttag agttgtagac
     5881 caatgggtat atcattcaag attgtataaa gcagctagtt ttgtaagaga taaaaactat
     5941 ttggaacttg ttcaacttaa ttcatttggc tgtggattag atgcagtaac tacagaccag
     6001 gttcaagaaa tacttaatga aaaatcgaag atatatacta ttataaagat tgacgaaggc
     6061 aacaatttag gagcagctaa gataaggatg aggtccttaa aagcagctat gtttgagaga
     6121 gaaagcaaaa atatagatgt aaaaaatata aaggtaaaca ggatacagta tgctaaaaat
     6181 aatcagataa cgaacaaaca tactatatta gcaccacaaa tgtctccaat acatttccaa
     6241 ttccttgaaa aagctgttaa cttaagtggt tataatatag aaattctaaa agatactgat
     6301 agtagtgtaa ttgaagaagg gttaagatat gtaaataatg atgcatgtta tccagctata
     6361 atagttgtag gtcagctaat aacagcatta aaatctggca aatatgattt aaataacact
     6421 tctgttacta taactcagac tggtggaggt tgtagagcta caaattatat tggattctta
     6481 agaaaggcta tatacgatgc tggatttaaa gatattcctg tcattgcact tagtgtaaat
     6541 ggaatagaag atagtggtat aatggataat atatcactaa aattaatcaa tagactcttt
     6601 atgtctgttg tttatggtga tttgctgatg aaagtactat atagagtaag accatatgaa
     6661 aaagttactg gaagtgcaaa tgctctatat gaaaaatggg ttgatatatg caaatcttct
     6721 cttgttaaag caaaaatttc tgtatttact agaaatataa aagatatagt aaaagaattt
     6781 gataatttag agatactaga tatcaaaaaa ccaaaagtag gtttggttgg agaaatattg
     6841 gtgaaattcc atccaattgc aaataataat ttagtagata tattagagcg agaaggtgct
     6901 gaagcagtcg tgccagacct tacaaatttc ttcttaagtt gtgcctttaa tactatatac
     6961 aaacacactc atttagaagg aagtagaaag agtagaatga taggagaagc atttatatac
     7021 ataactggaa tatatcaaag agtatataaa aaagcacttg ataagagtga gagattttat
     7081 gctccagcca atataaaaaa tgttgctaaa agtacagaac cagtagtgtc attaggtaat
     7141 caaactggag aaggttggct tttaacaggt gagatggttg aattattaaa tgaaggtgta
     7201 gaaaacatta tttgtatgca accttttgga tgtttaccta atcacataat tggtaagggt
     7261 tctataaaag aattaaagag gttgtataaa aatgcaaata taatacctat tgattatgac
     7321 ccttctgcaa gtgaggtaaa tcaacttaac aggataaaac ttatgctttc taaggctttt
     7381 aaaaatatat aagatttata ttttgatatg ttaaaagtta atgaatttaa aaatatactc
     7441 tatattaagt atctataaaa aagagttatc ctatttttta gggtaactct ttattagtta
     7501 aaaaattata tgtatactga tattaaaggg gggaagtaat atgagtttag tatatgatgt
     7561 ttataaatca aaattaggaa atttgtacat attaagtgaa gatggtttta tagttagtat
     7621 atacatagga gatgaaaaat ttaataattt aaaaaatgaa tgtattaaaa aaggaactga
     7681 tataaagaga agtaaggaag atttaaagga agcaataagt cagatagatg attactttaa
     7741 tggaaaaaga aaagtcttta atttaaatat tcgtattgaa gggactgaat ttcaaaaatc
     7801 agtttatagg gaaatgttaa aaatacctta tggagaaaca ttgtgttatt cagatatagc
     7861 aagaaatata aacaatccaa aatcagtaag agcaatagga caagcaagta aaagtaataa
     7921 aattccaata ataatacctt gtcatagagt agttggcaaa aaagatattg gtggatatat
     7981 ggggaatcat tctgatttga aagagatact attaaattta gaaagacaga gttaacttag
     8041 aaagaaatgt ttttattgtt aatatttgat ataataatag taaaaagaag tattttttac
     8101 aaaaataata aggaatttat tttaatttac aaaaatgagg ggggatttta atgtgtgata
     8161 aagttatagc atcaaaatat catgagaagg gatttaattg tgcagaatca gttataaaag
     8221 catataatga ggaatttaat acagatatac cagtttgttt aggaagtgga ctgggttcag
     8281 ggtgtggggt tgctagttta tgtggtgcag taaatgcatc aaatataata attggatatg
     8341 taaaaggcag aaaccacgaa gatgaaagta ctaaagctaa agtatatgct aaagatttaa
     8401 ctacaacagt aagaaaagag tatggttcag agttatgtat agacctaaaa aaagatttag
     8461 ttgcttgtag agaaataatg gatttcgcat atgatagtct aaaggaaact ttaaaaaaag
     8521 aattataaga aaataagcta tcaaatatga attatttgat agcttatttt tatagtcgat
     8581 actaaataaa tcaaatatat tttagaatta ttatttgtaa ataaacagca aataatttta
     8641 ggctttttat ttaatatcgt ttttatttat atttattttt gaatatgtta tactttgttt
     8701 aaataaatat ttgtaatgag ggagatgaga agattgaaga atctcaattt acttattaat
     8761 aaattatatt caaaaaatca taatgaagca tataaaactt ttttattctt agaaaatgaa
     8821 agtttaaaat caaatattac atattgtttt tttgactcat tcctagaaat gattgataac
     8881 gaaaactcat atattcgaac aaggggattg ttattaatct ctgcaaatgc tcagtgggat
     8941 attgataata aaattgaaat aaatatagat agtatactaa gtcatatcgt agataaaaaa
     9001 ccttctgtat caagaatgtt tattaaatcc atacctaata ttacaaagta taaagaaaat
     9061 cttattaatc gcataaaaat ggaattaagt aatgcagata taagtattta taataataat
     9121 atgaaaccat tagtagaaaa ggatataaac gatacattat ctaatattag ttaaatatta
     9181 atatagaaat tgaatattat ctagttacaa ttatttataa ctaagataat aaaacaacaa
     9241 tggtgctaat ttatatgtat aaattaatta tgaaaaattt atataagtaa aatatattaa
     9301 cataaaggta aaattttgta tactaaaagg gaatatttac agtttaatgg cagttttaaa
     9361 ataataaatt aaagctgtca tttttattta aaaattttta gaaaaagtac aatagtgtta
     9421 atgtgtgaat tagataaggt gtatataata atttagatga tatgatataa aatatgttgt
     9481 aaattaaatg ttttctttga caatatatac acaaggatga taatatctat atgaataaaa
     9541 gatagtgtgt aatttgataa tatgtaatta taacattatg atataattac aataaagtat
     9601 gtaataatct gtagaaattt caagctagtt atctaagttt tcggataact aaatctaaac
     9661 ggaatacata atgtattaaa taaatgatga tactaaaatg atactatttt tatagaaatt
     9721 tattgaggta tataattaat gaaaataata tattaaatta tataaattaa attatataat
     9781 atattgagtt ttacataaaa gcaaaggtgg gaataaaaat gaaagaaatt gctgatgcta
     9841 taacatcaga gtatttaagc tatggtatca atgaagatat tagtatctat gaaggtagat
     9901 tttgcatata tttagataaa aaatatagat gtaatgggaa aatttattat aaaatgacac
     9961 ctccaatatc tataagtttt aaagcagaca taggttgtgt tgaagagata gacaatgaag
    10021 atgacaattt agctttagat tatgataatg ctattcttga agttcatgga tataaaatta
    10081 tttctataac aataaatact ttaaatgaat ttagtgtaga aggttatatc aatgatgact
    10141 gcataaaatc taagaactcc tatgttgaat atgtagattt taatataata aacttagata
    10201 aaatacctgg aaaactcatt aaatacaatg ataaggtata cgcaggtaga attgaatttg
    10261 atataaatga ttatgttgta actatagaca agcgatatga ttatagaaaa gaattaaaat
    10321 cagaattaaa gtctaaatct ggagcaataa taactcatat aggtaggatt agaagaaaag
    10381 atggtagaat ttttagaaca aataatacaa taaatctttt agatagaata tcaacagctt
    10441 tgagttttat gtgtggaaga tatgtagggt tttgtttggc caaagggtat agaagtggta
    10501 atgaagttta tagaatatgg aatgaaaatc aaatatctcc ttttagatat gtaccaacat
    10561 ggtcagatac actttcaaat tatcataata tggaaaaata tataagcctt atgtgtaaaa
    10621 agctagaaga tttttattat ggttctgcta taaagagtgt tgttgattgg tacatagaat
    10681 ctcttggaag tgctaccatg gaaaacaata taatatctgt acaaattgca cttgaaactt
    10741 tgtcatatgt aattctagtg gaacaaaata agatacttac agatgaagca tttgactgta
    10801 atttggcatc taaaaatata agactcttac ttgatacatg taagatacca tatggtaaac
    10861 atgaattaaa tatatttgat aatataataa aaaataaatt tgatgatgga gtggatttag
    10921 tcatttattt aagaaatagc atagttcatc caagtagaaa gactcataga gctgtgttag
    10981 aggttgaaga tatttggaat ataatttcaa taggaacgag atatattgaa cttgttttac
    11041 tctttatttt aggttataga ggagaatatt caaacagatt agtagaaaga tgttatggtg
    11101 aagtggaagt tgtaccttgg aactagattt ttatatttat tttaaaatat atattgacat
    11161 atattgcctt atgctataaa atcaatatca taataataat tcagaataac attaaatgct
    11221 aagaaaagaa atagtaaaag tagttaatct taaacagaga gtttccgatt ggtgagagga
    11281 gcaatagatt ttactttgaa cacatctttg agcaggactc tgaaagagta gaaatacttt
    11341 attaggagat ttccggaatc cttgcacccg ttatagtgct agagtataat aattttattg
    11401 tacttaaaga ggttagtgtc gtgaggcatt aatgaacaga ggtggaacca cgagagttaa
    11461 ctcccgtcct ctagcagaag ctagaggcgg gagtttttta atataatttt ttaagcgcgc
    11521 agagaattat attgcaatag atacactttt taaaaaataa tcaaaatatt ttaaaaatta
    11581 aaaggcgagg gagaatatta tgaaaaatat attaaaaaaa gtaggaatat ttacaattat
    11641 gttaggttta ttaggtggag ttgtaggttg ttcaaaacca gataatgaaa aagataagga
    11701 tgcttcaaag gaatctaaaa aagaagtagt tgtagggttt gataatacgt ttgtaccaat
    11761 gggattttta gatgaaaagg gagatacagt aggatttgac gtagacttag ctaaagaaac
    11821 atttaaaaga cttggaatgg aagttaagtt tcagcctata gattggtcaa tgaaggaaac
    11881 tgaattaaat gattcaaaaa ctgtagatgt attatggaat ggatattcta taacagacga
    11941 aagaaagaaa atagtatcat atacagaacc atatttacaa aataaacaga taatagttac
    12001 tctaagtgac tcaaaaataa attcaaaagc tgatttaaaa gataaagaag ttggaacaca
    12061 acaaggttca acagcacttg atgcagtgga aaaggataaa gattttatga atagcttaaa
    12121 aggtggagca ccagttcttt atgatactta tgataaagca cttagagatt tagagatagg
    12181 tagaactagt gcagttgttg gtgatgaagt tttaattaga tactatatgg gacaaaaggg
    12241 tgaagacaaa tacaaggtat taaaagacga ttttggatta gaagattatg ttgtagcaac
    12301 ttctaaagaa aatccagaac tttgtgagaa aataaatgaa acattaaaag aaatgaaaaa
    12361 agatggaact tttgacaaaa tttatgataa gtggtttaaa tagaagataa gttagctgtt
    12421 aagaaggggg cactaaaggc atgttacaag gtttagagat agttatagca atgttttgta
    12481 taacattgat agtatcaatt ccattgggga ttggagttgc atttttaaga ttatcaaaga
    12541 ataaattagt aagtggtatt acacaatgtt atatattgat aatgagaggt acacctttac
    12601 ttttgcaaat gatagtcata ttttacggat tacctctttt aggaattgta tttgacagat
    12661 ttacagctgg agtagtagct ttcttcttaa attatgcagc atattttgca gaaatattta
    12721 gaggtggaat acagtcaatc gatagaggac aatatgaagc ttcaaaagta ctaggatttg
    12781 ataagtttac tatgtataag agagtaatct ttccacaagt ttttaaaaga atactagcac
    12841 ctatttcaaa tgaagtaatc acattagtaa aagatacatc tttagtatat atattaggat
    12901 taaatgatat tttaagaata tctcaaatag caatgaacag ggaagcaagt ctgttaccat
    12961 tatttgaggc aggagcgata tatcttatat ttgtagctat acttactaag ggatttgaat
    13021 tacttgaaaa aaaatattct tattatagat aaagttacaa tattatagga ggaggttttt
    13081 tatatgttga aaatcaaaaa cttaaataaa tcatttaaaa aaaatagagt acttaaagat
    13141 atttcttttg agttagaaga aggtcagatt ggagttttac ttggtaagtc tggtgcagga
    13201 aagaccacaa tattaaggtg tataaatggt cttgaagaat ttgatagtgg agagattata
    13261 atagataatg aggtaataaa aaataagaga gatatggcaa aaatacgagg aaaaataggt
    13321 atggtttttc aaaatttcaa cttatttcct catatgacag tccttgaaaa tataattgaa
    13381 tccccagtta atgtttttaa agttcctaga aaagaggctg aagagagggc tagagaactt
    13441 ttaagattag ttgatttaga agataaatta aattcttatc catttgaatt gtcaggagga
    13501 cagcaacaga gagttgctat agctaggtca tgtgcattga tgccaaaagt cctttgtttt
    13561 gatgaaccta cttctgcact tgatattgat actattcaaa gagtagtcaa tataatgaac
    13621 agacttaaag acaaaggtat gacaatatta ataattacac atgatgttgt cttctcaaat
    13681 aatgttgcag ataaaattat aagtataaaa gatggtatag tagaaaatgt tcaaataaaa
    13741 gaaaaaattg tttagagttt tataaatata aactagggta gaattttata atataaataa
    13801 agggcaagta aaatcaaata tactatgccc ttttgttatc tcaaaattta atcttatatt
    13861 ttatgtttaa gttacattat aat
//
LOCUS       DAEORZ010000112        13608 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEORZ010000112 DAEORZ010000000
VERSION     DAEORZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.113"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            74..214
                     /locus_tag="KRH39_002818"
     CDS             74..214
                     /locus_tag="KRH39_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512707.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(389..709)
                     /locus_tag="KRH39_002819"
     CDS             complement(389..709)
                     /locus_tag="KRH39_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG8512708.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(829..1566)
                     /locus_tag="KRH39_002820"
     CDS             complement(829..1566)
                     /locus_tag="KRH39_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBG8512709.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1781..3604
                     /gene="acd"
                     /locus_tag="KRH39_002821"
     CDS             1781..3604
                     /gene="acd"
                     /locus_tag="KRH39_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBG8512710.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3819..8117
                     /locus_tag="KRH39_002822"
     CDS             3819..8117
                     /locus_tag="KRH39_002822"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBG8512711.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8321..8782
                     /locus_tag="KRH39_002823"
     CDS             8321..8782
                     /locus_tag="KRH39_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBG8512712.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8812..9966
                     /gene="nusA"
                     /locus_tag="KRH39_002824"
     CDS             8812..9966
                     /gene="nusA"
                     /locus_tag="KRH39_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBG8512713.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            9973..10254
                     /locus_tag="KRH39_002825"
     CDS             9973..10254
                     /locus_tag="KRH39_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBG8512714.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10244..10555
                     /locus_tag="KRH39_002826"
     CDS             10244..10555
                     /locus_tag="KRH39_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBG8512715.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10577..12517
                     /gene="infB"
                     /locus_tag="KRH39_002827"
     CDS             10577..12517
                     /gene="infB"
                     /locus_tag="KRH39_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBG8512716.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12560..12940
                     /gene="rbfA"
                     /locus_tag="KRH39_002828"
     CDS             12560..12940
                     /gene="rbfA"
                     /locus_tag="KRH39_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBG8512717.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            12921..13598
                     /locus_tag="KRH39_002829"
                     /pseudo
     CDS             12921..13598
                     /locus_tag="KRH39_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
BASE COUNT         5437 a         1541 c         2561 g         4069 t
ORIGIN      
        1 gaaataataa taacagatat tataattaat taacagtttt tgtaagtatt tgacaataga
       61 gagggtgtaa caaatgggaa taatagagtc agcatcaaaa ttggctgaga tggttcattt
      121 attggctgtt gaaaagggga taactgatat agaagcatgg gatgaagctg tgaaagaata
      181 cagcaaaatt tatgaagaaa gaagaaatga atagaatgat aaaaaatgaa aacttgatga
      241 taaaaaataa ctttatttaa agaaattcaa tttttataga aatattaaaa ataatataaa
      301 taaaaagatg gtatttcaaa tgtaagtaaa aaatcatttt gggataccat ccttattatt
      361 tattttaata atatataagt aataaatctt acttatccat attttttatg taacctcttt
      421 tttctagctt ttttatatgc tccttgcgta tttcatcaag attaatacca tattgttctt
      481 ctagcttaaa catcatagta acacatgtct gagctacatc cattagttcc ttagcagttt
      541 tctttataat atcaacttca tctaaatcat tattttctcc acttagattt ctgaatttac
      601 ctatacattc agctaattca ccttgttctt cacccatttt gataaatgta gattctagtg
      661 ttgggtctaa attattaagt tctggtaaag agatagtttt tagtttcata aaagctcctt
      721 tcaagttatt cgtatttata ctaaaacatc ataacaaaaa taatactaaa aaaaaacttt
      781 aaattcaaca taaaaactta catatataat aaaaatcgtg aaataaatct atatttgtct
      841 taatacagcc tcatataaaa cttctccaat aggtttattt attacaagag aagctgactt
      901 atcatagact gtatttgctt tattaataag agcaatataa tcacctttgt aataatctat
      961 aaaacttgct gctgggtaca cagctaatga tgtacctcct ataatcaata aatcagcatt
     1021 acttatagca gaaattgttt tagtaatgac atcactgtca agagcttcct cataaagtac
     1081 tacatctggt tttactatag aaccacagtt gtcacagtaa ggaatattac ctcccagatt
     1141 taacatagat tctaagtcaa aaaacttacc acatttagta caataatttc tatgtacaga
     1201 accatgaagt tctagcacat ttttacttcc agccatttga tgaagtccat caatattttg
     1261 agttatgaca gctttaagct tacccatttc ttcgagtttt gctaaagcta tatgagcatt
     1321 attgggtttt gcattaggat atattaattt atccttataa aattcaaaaa attcttctgg
     1381 atatctaaca aaaaaagtat gtgaaaccaa ttgttcagca gtaaattgtt tattgagttt
     1441 ttggctaaat aatccagtag aacttctaaa gtctggaata ttagattcag ttgaaacacc
     1501 tgctccacca aaaaatacaa tattattgtg attagctatt aagtctttta agctatttgc
     1561 atccatagtt acacctccaa attattatat ataaattgta ctacatatta ggtcgtttat
     1621 gttttattaa tttaaataaa tatatagttt aataagttaa ataaagggaa tataaataaa
     1681 atgtatacag agtataaata gacaatattt actaaaataa caagttgtcg tataattata
     1741 ttaaaattgt tttagtgtat aagaagggcg ggggaagtac gtgaagaaaa aagcagcttt
     1801 agcaacattg gctatgttac ccttaggtgt ggtaaatgca catgctgatg gagatatagg
     1861 tatagtgact ataaattatt taaatgtaag aaatgaacca actgccgaaa gtagcatagc
     1921 ctttgttgct aaaaaagatg ataaagtttt gattaaagac tcttctaatg gatggtataa
     1981 aataaaagct gaatctggac aagaaggttg ggcttcatca aaatatatag caaaatcaaa
     2041 tagtgactct ttaagaacgt ctacaaataa agaaaaacaa gttatttcaa atagtttaaa
     2101 tatgagaaat ggagcaggta caagttacag agtaataact gtactaaaaa aaggtcaaaa
     2161 agtagaagta atatcagaga gtaatggatg gtctaaaatc aaatatgatg gaagactagg
     2221 atatgtatct agctcttatt taggagacgt ttcaaactca actaataaat caaaaactaa
     2281 acaagtgaat acaacttcac taaatgtaag gagtggacca aatacaagtt atggtttgtt
     2341 aggcaagtta ccaaaaggaa gtaaagtaga agtaatatca gaaagtaatg gatggtccaa
     2401 aataaagtat aatggaaaag atgcctatgt atctagtatg tacttatcgg atgtaagtca
     2461 aagtaattca gacaattcta gtcaaagcaa tgataaaaag aatactgata aggttgtaaa
     2521 tacagcttct ttaaatgtaa gaagtggacc aggttctaca tatagtaagt tgggaaaagt
     2581 ttataaagga agtaaagtaa ctgtactatc agaaagtagt ggatgggcta agattaattt
     2641 taacaataaa gaagcatttg tagtaggcaa ttatttatct acttcagcag atacttcaaa
     2701 taataactca aatagtaact ctgataacag ttctaatagt aatggtaata attcttcatc
     2761 atcaggtcaa gtaaatggta tgtcaggtat aagtggggct aaaattgatt ataaatcttt
     2821 gagttatact ttagaatctc atataagtaa acaagttgag aaagcagcat caggaggaaa
     2881 tgtgatagct ccaagtaata ggaaaagcac tccaagtcct gaatttagta ctttttcagc
     2941 tcaaagaaca agctcatttg taaatgcaag ttcaagtgat atagaatatt atttaaatcc
     3001 taagaatttt acaaatacta ctaaaggtat gatgcagttt ttaaagatta acagttatag
     3061 agatggtatt tcagaatcaa gtcttaactc gtatcttaat ggtttatctt ctagtgtatt
     3121 taaaaaccag ggagcagcct ttataaatgc tgccaagaag tataatatag atgttgtata
     3181 tctagtatct catgctatgt gggaaactgc ttatggtaag tctacacttg cccaaggtca
     3241 aacattaaca tcttataagg gacaacctct tagtaagcca gttaaagtat acaatttttt
     3301 tggtatagga gcaatagata aaagtgctaa tgtttcaggt gcagaagcag cgtattccaa
     3361 tggctggact agtgtagaag ctacaataga tggctctgca aagtggatat cacaaaatta
     3421 cgtaaatagt tctaagtaca atcagaatac tatctacaag atgaagtgga attatgatta
     3481 tacatggcat cagtatgcaa ctgatgtaaa ctgggctaat ggaatttctg gaattatgga
     3541 aaatttaatt ggtctttatg gaggaggaag tagtttggtt tttgaagttc ctcaatacaa
     3601 gtaatacata aataaaaaat atagactatg ggtagttttc attttattta aattatctat
     3661 agtctttttg tatataattt ataatctgta aatttaatgt taacaaaact tttatgttat
     3721 aatattaatt ctaagtaaat ataagtgagt aataggtaga aatagataaa aagtggtaat
     3781 aatatatata aagataattt tgagaaagga ggatatatat ggagagtatt aaggaatatt
     3841 tggataagtt agaaattaat aatagtggtc ttggaaagca acttaaagaa gtttatataa
     3901 atagagtagt ttattttaag gaagataaga tagtttattt ttatctaact tctaaagata
     3961 ttgtttctca tgaactttta gataaattta aagaagaatt gatgtataaa ctcgattatt
     4021 ttaaagatat gaggatgaag ataagattta ctggtcttga aagaaaatcc aataaagatg
     4081 ttataaaaaa atattggaat aatatattgt atatattaaa gtatctttgt ccatctattg
     4141 cagggtggta taaacaagta gaatttcttt gtttagaaga agaattaaaa ataaaacttc
     4201 caaaaggtat attttatgag cgattaatga aaaaaaatgt aacttatgta cttaaaaccg
     4261 tgcttagcga agagttggga ttagatttaa atataacaat agaaaaagct gttgatgaaa
     4321 aggtaaataa agaaagactc ataagaatca atgatagaga aatggaagaa aaaattaggg
     4381 ctttagaaat tggaaaagtt aataactgtg aagaaaatga agaggaaagt tatgtcatta
     4441 agtctgatgt agatgaaaat ttaatctatg gagataatgc aaatgctatg attgaaaata
     4501 tagttgaatt aaatgcatct tctggaacag ttgcagttgt tggagatata tttgatgttg
     4561 acactaaaga acttaaaaac ggaaaaatac ttatgatagc ttcaattaca gattatacta
     4621 gctcaataag ctgtaaactt tttttaacag atacaaataa ggatggagta cttgaaaatg
     4681 ttaaaaaagg agcatactta aaaatcaagg gtgacatagt ttatgataca tatcaaagag
     4741 aaattagtat gatgattagt gggataagaa aagaaactag aatagagaga gaagataaat
     4801 cagaggaaaa aagagtagaa ctccatgcac atacacaaat gtcttctatg gatgctattt
     4861 gttctgttaa aaagcttgta gagcgtgcag ctaaatgggg tcattctgct attgctataa
     4921 cagaccatgg tgttgtacaa ggatttccag atgctatgaa tgcaggtaag tcaaataata
     4981 taaaaatact ttatggagta gaagggtact tggttgaaga tgattctctc attatagagg
     5041 atgcaaatga taaagaactt tcacagacat ttgttgtatt tgacatagag acaactggtt
     5101 tttctaatac aaatgataaa ataactgaga ttggtgctgt aaaaatagaa aactttaaga
     5161 ttgtagatag atttagtgag ttaataaatc ctgaaaaaga tatttcttat aagatacagg
     5221 aattgacagg aataactaat gaattaatta aagacaagcc aaccattgaa gaagttcttc
     5281 ctaagtttat ggaatttgtt ggagatagtg tattagtagc acataatgca gagtttgata
     5341 ctggatttat atcgcaaaaa tgtagggaac aaggcttgtt atataataac aaaaaagtag
     5401 atacacttat gttagcaaga gtaatgttgc caaacttaaa aagacataga ttaaatgtcg
     5461 ttgcaaaaga acttggtatt cctcttttaa atcaccatag agcagtggat gatgctgagg
     5521 ctacagcact tatatttaat aagtttttgc agatgcttac agaaaaaggt gcaaaaactc
     5581 taagtgatgt aaacaatata cttggaaaga ttgattacac aaagcttagc acaaatcata
     5641 taaccttgat agcaaaaaat tctataggta taaaaaattt atataaaata atttcagatg
     5701 cacatgtaaa tcattttttt agagcaccaa gaatccttag aagtgtatta aatgaatata
     5761 aagaaggatt aataattggt tcggcttgtg aagctggggt ggtttttcaa gctgtaaaga
     5821 aaaatgtgtc tgatgaggaa atgaaaaaaa taatagattt atacgattat attgaagtaa
     5881 tgcctataga taataataga tttatgattg ataaaggaga agtaaaggat gaagaagagt
     5941 taagggagtt gaataggaaa ttaattgata cagccaaaaa atttgacaaa attccagtag
     6001 ctactgggga tgttcatttt ttagacaaac atgaagctgt cttaagaaaa gttcttaaat
     6061 attctcaagg ttttaaagta gatgaagaag aaacttattt acattttaga acaacagatg
     6121 aaatgttgga agagtttagt tatcttggag aagaattggc atatgaagtt gtagttgaaa
     6181 atagtaatct gattgccgat atggttgaag atataaaacc tataccagat gatacttatc
     6241 ctccaattat agagggctca gatgtggagt taagagaaat gtgttacaaa aaagccaaac
     6301 gaatctatgg agacccaata ccagaaatag ttcaaaaaag actggataga gaattaaatt
     6361 caataataag caatgggtat gcagttatgt atattatagc tcaaaaactg gttgcaaaat
     6421 ctctttcaga tggatattta gttggttcaa gaggctcagt tggctcatct tttgcagcta
     6481 ctatgagtga tataacagaa gttaatcctc ttccagcaca ttatatttgt gaaaacgaag
     6541 actgtaaata ttcattcttt tatgaaatag gtgaatgggg ctcaggtgtt gatttaccag
     6601 acaaggattg tccaaagtgt ggaagaaaac ttgctaaaaa cggtcatgat attccatttg
     6661 aggttttctt aggttttgaa ggtgataagg aaccagatat agacttaaat ttctcaggaa
     6721 attatcagcc aacaattcat aaatacacag aagaactatt tggagagggg tatgtatata
     6781 gggctgggac tataggtact gtagcagaaa aaacagcttt tggatacgca agaaaatatg
     6841 tggaagaaaa taatataagt gtaccaaatg cagaagtact taggctttca aatggatgta
     6901 ctggagtaaa aagaacctca ggacaacatc ctggtggagt tatggttata ccagactata
     6961 aagatgtata cgattttaca cctatacagt atccagcaaa tgatacttct tgtggtgtta
     7021 taactactca ctttgattat cattcaataa gtggtagaat acttaaactt gatatacttg
     7081 gacacgacgg tcctaccatt ataaggatgc ttgaagatat gactggaata aatataacag
     7141 aaattccttt agatgataaa gagactatgt ctctatttac atctacagaa gcattaggtg
     7201 ttacaccaga agaaataaac tgtcctatag gttgccttgc tgtaccagag tttggaacta
     7261 agtttgtacg acaaatgtta cttgacacaa aacctactac atttgcagaa cttgtacgta
     7321 tatctggtct ctctcatggg acagatgttt gggtaaataa tgcacaagac ttagttagag
     7381 aagatatagt tggattaaaa gatgttatat ctacacgaga tgacataatg aactatttaa
     7441 tatttaaagg gttacctccc aaaatgagtt ttacaataat ggagagtgta agaaaaggta
     7501 aaggtttaaa accagaacac attgaagaaa tgaaaaaaaa taatgttcca gaatggtata
     7561 taggctcatg taaaaagatt aagtacatgt tcccaaaagc acatgctgtt gcttatgtaa
     7621 tgacatcatt tagaattgca tattgtaaag ttcactatcc agaagcattt tatgcgactt
     7681 attttacgac aaaagttgag gattttgatg cagatttaat tgttaaagga ttagatgcta
     7741 taaaatcaaa aataaatgaa attgagtcac tgggaaatga tgcaaccaca aaagaaaaag
     7801 gtatgttgac cgttttagaa gttgctcttg aaatgtatgc tagaggaata aaattattac
     7861 cagttgatat atataaatca gatgcaactg agtttatagt tgtaggagaa aaaacattac
     7921 ttccaccaat ggcagcaata caaggtcttg gaggaaatgc ggctataaac atccaaaatg
     7981 aaagaaaaaa tggtgaattt atatcaaaag aagatttgag aaagagaaca aaaatatcaa
     8041 aaacggtaat agaaactctt actaatcatg gttcacttga gaatatgagt gagagaaatc
     8101 aattgtcatt gttttaagtt agcccaaaaa gttgcaaata aaagaatttt atgatatcat
     8161 agtatagaaa atagtatact ttgcagaaac taagagtgag acagctgttc tcactctttt
     8221 attttaaata tattattaaa aagctggttt atttttatta aatatatttt atttaagatt
     8281 atataaactg gtcatttaca attgaatagg aggaacttta atgaaaaaaa atttagaagc
     8341 tacaattgag gagattgtaa ctaaaataac ggatgaacat ggatttgaaa tggttgatgt
     8401 tgaatatgta aaagaagcag gagaatacta cttaagagta tacatagata aagaagaagg
     8461 aatatcttta aatgaatgtg aattagttag tagagaatta agtccaatac tagatgaaaa
     8521 agaccctata aaagaaaatt actttttaga ggtatcttct ccaggacttg atagagcatt
     8581 aaaaaaagac agagattttg ttagatatca aggtagagat gtagatttaa aattatataa
     8641 accattaaat ggatgtaagc aatttgaagg tgaacttgta ggattaacag aagataataa
     8701 tataaaaatc attgcaaatg gtaaggaaat ggaatttaat agaaaagatg ttgctattgt
     8761 cagattagca ataaagtttt agtggtatat taaataggga ggatataaaa aatgaatcat
     8821 gaatttatgg aagcattaga tgaattagta gttgacagag gtatagataa agaaatactt
     8881 atagatacta tagaacaagc tcttttaact gcatacaaga aaaactttgg ttcagcacaa
     8941 aatgttagag ttgaatttga tagagacaag ggagatataa aagtattttc tcaaagagtt
     9001 gttgttgatg aatcagattt atatgatact ttcttagaaa tagagttagc agatgctaga
     9061 gagataaacc ctaattatga attaggtgat attatagaac atgaagttac acctaaaaac
     9121 tttggaagaa ttgcagctca aacagcaaag caagtagttg ttcaaagaat aagggaagct
     9181 gagagagaaa tagtgtataa tgaatttata gaaaaagaaa atgaaatagt tacaggagag
     9241 gttgctagag caaataaaaa tgttgtttat gtaaatcttg gaagaataga aggtataatg
     9301 actcaaagtg aacaaatacc atttgagaat tataaagcag gtcaaaaaat aaaattctat
     9361 atacttgaag ttaaaaagac taacaaagga cctcaaatag ttgtatcaag aagtcatccg
     9421 ggtctagtaa agagattgtt tgaagaagaa gttcctgaaa tatatgaagg aatagttcaa
     9481 ataaaatctg tagcaagaga agcagggtca agaactaaaa tggcagtaag atctatagat
     9541 gaaaaaatag acccaattgg agcttgtgtt ggaccaaaag gacttagagt aaaaaatata
     9601 gtggatgaac ttggcgatga gaaaatagat ataataaaat atagtgaaga cccagcagag
     9661 tttatatcag cagcacttag cccatcaaag gttgttagag tagatataga tgaagatgaa
     9721 aagtctgcct tagtaatcgt tccagattat cagttatcgc ttgctatagg taaagaaggt
     9781 caaaatgcaa gactagcagc aaaattgact aactggaaaa tagatattaa gagcgaatct
     9841 caagcagaac aagaaatgtt aaatcttgaa aattccaaag aaatagaagc tgaggatgtt
     9901 gaagctgaat ttatcgacca agaagagtta gatacttcag aggataattt tagtgaagaa
     9961 gagtaaggag ggatgatttt gaaaaaagta aaaaaagtgc ctcaaagaaa atgcatagca
    10021 tgtcaagata gagactctaa aaaagagctt ataagaatag ttaaaaataa agaaggaaaa
    10081 atatttttag acccaacagg aagagccaat ggaagaggag catatgtatg taaaagctct
    10141 gaatgtctaa aaaaagctat aaagagtaaa gcattaaata aagcttttaa aatagatgtt
    10201 cctgatgaag tctatgataa tctacttatg gagttagaag aatatgaaaa ataataaaga
    10261 aaaagtactt tcgttcttag ggctcgcaac tcgtgcaggg aaaatagtgt ctggtgatga
    10321 ttcaactttg cttgacctta aaaaaggaaa agtaaatttg attttaatcg cagaagatgc
    10381 atcaaataat actaaaaaac tttttaaaga taaatcaact tttagaaata ttccatatct
    10441 atttttttca acaaaagagg aaataggatt tgcaatagga aaatctccaa gagcagttgt
    10501 aggaataaaa gatgaaaatt tttctaaaaa gataattgaa ctaatagaaa tttaaaataa
    10561 aatagggggt gatcttgtgt caaaaacaag agtataccaa atagcagaag aattaaatat
    10621 atcaaatgaa gagcttataa ataagttagc tgaattagat ataaatgtaa ctgacaaaga
    10681 ttcagtatta gaaggtgaag agttagaatt agcacttgaa atgttaggtg aagatttatc
    10741 tcaagaaaat ggaaatgtta tagaaataga tggaaagttg acagtacaag tattagcaac
    10801 gaaattagat aaatctccat cagaaataat tatgaagctt atgaaaatgg gaactatggc
    10861 aacaataaat caagagataa gttttgagat tgctgccttg gctgccaaag actatggatt
    10921 tgaattaaca gtagctgaaa gtgatgatac agaagcttta gaaatagaag ctttaatgga
    10981 aattgaagaa gataaagaag aggatttaaa acctagacca ccagttgtta cagttatggg
    11041 acatgttgac catggtaaga catctttact agatgcaata agaaaaactg atgtgatttc
    11101 aggtgaagct ggaggaataa ctcagcatat aggtgcttca gaagttaaaa tcaatggaca
    11161 caaaatagta tttttagata ctccaggaca tgaggctttc acatcaatga gagcaagagg
    11221 agcacaagtt acagatatag caatattagt agttgcagca gatgatggta taatgcctca
    11281 aacagttgaa gctataaacc atgctaaagc agcaggtgta cctttaatag ttgctattaa
    11341 taaaatagat aaaccaggtg caaacccaga taaggttaaa caggaattag cagaccaagg
    11401 attattagtt gaagactggg gtggagaagt aatagcagtt ccagtatcag ctaagaaaaa
    11461 agaaggaata gatacacttt tagaaatggt attattggtt gctgaaatgg aagagttaag
    11521 agctaatcca aataaaagag cagtaggaac tgttatagaa gctgagcttg ataaaggaag
    11581 agggccagtt gcaacagtac ttgttcaagg tggtacttta acagtaggtg acccaatcgt
    11641 tgcaggtgtt gcatgtggta aagttagagc aatgataaat gcaaaaggta agagagtaaa
    11701 aacagcagga ccatctacag cagtcgagat attaggtcta tcagaagttc cacaaggagg
    11761 agaccaattt gtcgaagttc caactgataa gattgctaga agtgtagcag caagaagaca
    11821 acaaatagtt agagatgaaa tgttaaaatc aactcaaaga ttatcactag atgctctatt
    11881 tagtcaaatg agtgaaggtt ctataaaaga ccttaatata gttataaaag cagatgtaca
    11941 aggttcagta caggcagtta aacaatcttt agaaaaatta tcaaatgaag aagttcaagt
    12001 taaagtaata catggtggtg ttggagctgt aacagaatca gacatattac ttgcagcagc
    12061 ttctaacgca ataataatag gattcaatgt aagaccagtt ccaggtgcag aatctctagg
    12121 agaaaaagaa aatgttgata taagaacata tactataata tacaaggcaa tagaagatat
    12181 acaagcggct atgactggta tgttagaccc tgaatatgtt gatgaagaaa caggaaaagc
    12241 tgaaataaga gaaatatata aaatatctgg tgttggaaca gtagctggat gttatgtgac
    12301 taatggtaag atatttagaa attgtaaagt aagattagtt agagatagca taataataca
    12361 tgaaggtgag cttgcagcac ttaagagatt taaagatgat gtaaaagaag ttaattctgg
    12421 atacgaatgt ggtatgagtt ttgtaaatta taacgacata aaagaaggcg atatagtaga
    12481 agcttatata actaaagaag tagaaagaaa attataaatt cgtatcaaag aaaatcagtt
    12541 agtaaagaag gtcgatggta tggcatcata taatagaaca agaaggatag cagaagaaat
    12601 aagaaaagtc gtaagtacta tgcttataaa tggagtaaaa gaccctagaa taacatctat
    12661 ggtgagtgta actgatgtag aagttacaaa tgacctaaga tatgcttatg tatatgtaag
    12721 tattctaggc ggagatgaag aatccacact aacaggtctt aaaagtgctg gaggatatat
    12781 aagaagagaa gtaggaaaaa atataaaact tagatatatt cctgagatag tatttaaact
    12841 tgatgattca atagaaaaag gaatgtatat ggatagcctt ataaaaagag ttaatgaaaa
    12901 aaatgctcaa caaaatgagg atgaaaatta tgatgaataa tatggatagt attataaact
    12961 atatttatga gggtgactat tttatagtta cctctcatat cagtccagat ggagacaata
    13021 ttggttctac actttcaatg tattatacat tgaagaattt aggaaaaaat gtatattatg
    13081 tacttgatga tgaagctcca ttaaatttaa ggtttttggt tgaaggtgta aaaatctata
    13141 agtctaatga atttaatatg aagaactatt ctctaattgc acttgattgt ggagataaga
    13201 tgagaatatg tgtgagtgat gaaatcaaaa ataatgcatc aaaattaatc tgtattgacc
    13261 atcatgctag taatgattca tatggagatt taaattatgt agatattgat gcatcttcta
    13321 catgtgaatt agtttataat ttattagtta gatttcagca aataaaagat attaatatta
    13381 taaatgaaga tatagcaaca tgtttatata caggtcttgt aactgatact ggaaactttt
    13441 cgtattcaaa tgtacatgca agtagctttg aaatggcaaa aaatttactt gttttaggtg
    13501 cacaaaaaaa tactataata caaaatatat atcaaagcaa ttcttctggt tattacaaat
    13561 tattaggaga agctctaaag ggtttggaga tatttgatta aaaaatag
//
LOCUS       DAEORZ010000113        13507 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEORZ010000113 DAEORZ010000000
VERSION     DAEORZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.114"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            950..2308
                     /locus_tag="KRH39_002830"
     CDS             950..2308
                     /locus_tag="KRH39_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG8512718.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2415..3209
                     /locus_tag="KRH39_002831"
     CDS             2415..3209
                     /locus_tag="KRH39_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG8512719.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3202..5508
                     /locus_tag="KRH39_002832"
     CDS             3202..5508
                     /locus_tag="KRH39_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG8512720.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5647..6924
                     /locus_tag="KRH39_002833"
     CDS             5647..6924
                     /locus_tag="KRH39_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG8512721.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNIKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7107..7829
                     /gene="yqeC"
                     /locus_tag="KRH39_002834"
     CDS             7107..7829
                     /gene="yqeC"
                     /locus_tag="KRH39_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBG8512722.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSPTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KECESEIEIFYGSVKQKFCVRY"
     gene            7936..8505
                     /gene="mocA"
                     /locus_tag="KRH39_002835"
     CDS             7936..8505
                     /gene="mocA"
                     /locus_tag="KRH39_002835"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBG8512723.1"
                     /translation="MINAVIMASGFARRMGVNKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            8502..9521
                     /locus_tag="KRH39_002836"
     CDS             8502..9521
                     /locus_tag="KRH39_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBG8512724.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            9859..11484
                     /locus_tag="KRH39_002837"
     CDS             9859..11484
                     /locus_tag="KRH39_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8512725.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            11683..12159
                     /locus_tag="KRH39_002838"
     CDS             11683..12159
                     /locus_tag="KRH39_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512726.1"
                     /translation="MVKKRLVAVVLSLAMISVGDFSYAHSGGHNSSGGHHNKNKSELT
                     HYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(12367..13479)
                     /locus_tag="KRH39_002839"
     CDS             complement(12367..13479)
                     /locus_tag="KRH39_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG8512727.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
BASE COUNT         5276 a         1426 c         2426 g         4379 t
ORIGIN      
        1 gtatatttta tgttgcagat acaaatatag taaaggagag tacattggta caactgagat
       61 acatagctta ttaagttttt ctgtcttatg agaattatat tataaaatat tcataaatat
      121 tttgagttta aattaaataa ttattttatg cttagattaa ttgatattta attaatttaa
      181 taacaaaaac agtgaaagag taaataatat tatttacagg gggtaaagca aagtatgtta
      241 aataaattgt acttttggcc ccgtacatag agaaatctat gtaggacaca actttaaaac
      301 cagtaaatcc taaagccttg caactacaat aaaagataga aagaaacttt tatgaatgtg
      361 gtgaaaacca gaaacaatag tgaggatggt atatggtgag agcctaagta cttaaaaatg
      421 gacatttggt cgccaagcct agaaatagga aggtcaaacg actatctcga gagagagtac
      481 actataagcg attgatagtg gaaacgggtt gctcttagca ggtaatgctg aagatgaaga
      541 aatagtctac gctcatatga aagtatgagg tcactggatt tgaagtgaca tctataaagt
      601 agcgtttata ggtaaatgtt taaaacttga aacatgtaaa tacttatgaa aacttataac
      661 ttataatgat taagattgaa agatttaaat tgaattaaat ttactataaa aaattaaaaa
      721 ttcatagcta taatactctt atatttgtaa agcatggtat ataatgtata caaaattaaa
      781 gaatacattt atttgttaat aattaaattt attgcatctg ttttttaaga ctaaaaactt
      841 aaatatatgt tggagtatag ttgatattta tgaacagcat taatttaata tattagattt
      901 ttaagtattt atataattca ataagtttta tgtaacggga gtttttatta tgaaagcaaa
      961 aagtacatca aaatttgaat tagacggaat accaccacta aaggaagcaa tacctttagg
     1021 gttacaacac ttaatggcaa tggtaatagg tagttgttta ccagcgatat tgattgcaaa
     1081 tgcagctgga cttaatcata agatgtcaac attattgatt caagccgcat tagtttttgc
     1141 aggattatca actttattac aattatatcc aatacctttg tttaaaggtt ttaaattagg
     1201 aggaggaata ccagttataa tgggagctac agctatgttt ataggagctg gggtatctgt
     1261 tgcaagtaag tatggactac cagtattatt tggctctcaa ataatagctg gtatagtaat
     1321 aattataatt ggttttggtc ttaaaaaaat acgatttata tttactccag ctgtatcagg
     1381 aacaatagtt gcatgtatgg gtcttggact ttttccaata gctataaaaa acttagctgg
     1441 gggtatgggc aatgagactt ttgggaatcc aattaatttt atagttggaa tatctgtatc
     1501 tcttatgata ttaggattaa ataaatatgg aaaaggatta tttaaagatg catcaatatt
     1561 agttagcata atttttggat acattttatc attaattttg ggtatagtaa atttttcttc
     1621 aattcaggag tttactttgg ttgcacttcc aaaaccactt gcttttgggc tagatataag
     1681 acttgaagtt gttgtaatgt tttcaataat atatttagtt gagatagctg atataatggg
     1741 tgcttgtaca ctttctgcgg taggagggct taatagacaa gtaacagatg aagaactttc
     1801 ttctgctgtt ttaggaaatg gagtactctc tacaataggt gcattatttt ctgcaactcc
     1861 aatggggatg tttggacaaa attctgcaat cgtttccaat acaaaggttg taagtaaatt
     1921 tgtactagct attggaggaa ttggattatt tttggcagga atatctcctt tattagctaa
     1981 tttaataaga acaattcctc cctgtgtggt tggaggagca acactcgtta tattttcaac
     2041 tcttacaaca tctggtttaa gacttgttag tatggatgga tttaatcaag aaaatagcat
     2101 gatattaggt ctttctatgg cttctggtat tggattcatg gtagctcctc aagttcttga
     2161 aaaatttcca aaatttatag agacattact tgcagattca agtgtagttt caggagcaat
     2221 ggtagccata ataattcaag ctttatatat ggtaaaattt cctggaaata aaagtgataa
     2281 ggttgaggat aaaaataaaa gtttataatg taaaggttta atatatgtga attaaatata
     2341 ctacaattat aaatataaat aaaagattta ttaaaaactt ttgtgtttta ataagttaat
     2401 gatgggggga taagatggtt actataaagg aatattcagt tccgcagagt ttagaagaag
     2461 catataaaat tttaatttct aaaaaaaata atgttatttt aggtggatgt ggatttataa
     2521 aattaagtaa taaaaatata ggaacagcga tagatttaaa agatataaat ctaaattata
     2581 taaaagagga tgaaaaaaat attttgatag gtgcagatac ttcacttaga agtttagaaa
     2641 ttgataaaac tataaaaaat tactgtagtg gaatactttc aagtgctgtt tctaatatag
     2701 ttggtgttca atttagacaa ggggctagaa ttggtgcaag tgtattttca aagtatggct
     2761 tttctgactt aataccagca cttttggttg ttggagcgaa ggtgagatta tacaataaag
     2821 gaatactaga gttaagtgag tttttggata gtgaattaag tagggatata ttggtcgaag
     2881 taatactacc aaaaaaagat gcaatagctg tttttgactc aataagaaaa tgcacaggag
     2941 atttttctgt attaaattca gcaatgctaa aagaaaatga tacttacaaa atagcaattg
     3001 gggcaagacc tcaaaaagct agattagcac ttgaagcgag taatatatta aataaagaaa
     3061 aagatataga taaggcatct attgttgcta gtaaggaact tacttatgga agtaatatga
     3121 gagctagtaa agagtacaga aaggatatgg caagtgcatt agttaaaagg atgtataatg
     3181 ctattgagga gggaatgtat aatgattaat aaggggataa gaaagataga tagtacagca
     3241 attgtcacag gaaaacctct atatactgaa gacctcatta tgcataaaga tgttttgaca
     3301 ttaaaactac ttagaagtcc acatgctcat gctaagataa agaatataga tacatcaaaa
     3361 gccgaaaaaa ttccaggggt tgtgggtatt tatacgtata aagatgtacc acaggtcaga
     3421 tattgtgttc caggtcaaac agaaccagaa gcatcacctt atgatagagt tttacttgaa
     3481 gatacagtta gatttattgg agatgaagta gctttagtgg ctgctgaaga tgagaaaaca
     3541 gcacttaaag ccttaaaaat gataaaggta gattatgagg ttttaaagcc tgtgtttgat
     3601 gcaagagaag cagaaggaaa tgaagttgtt atacatccag aaaaagacat agtagagcca
     3661 tacccttttg gattggatgc aaaaaataat atagtatcaa aagaattttt tgaatttgga
     3721 gatgttgaag ctaagcttaa agaaagtgat tttgtggttg aatcttcata caaaacccaa
     3781 agtcaatcgc attgcatgat ggaaactcat aggtcatatt catataaaga tataaatgat
     3841 agaattgttg tagtttcatc attacaatca ccacacaatg taaagagaat acttagaaaa
     3901 gtattgggta taccgtcttc aaaaataaga gtaattaaac ctagaatagg tggtggattt
     3961 ggaggaaaga atatagcagc aacagatata tttactgcat ttgttactat gaaaactggt
     4021 agaccagcaa aatttgtatt tacaagaagt gaaacttttg taggttctaa tagtagacat
     4081 gaaatgtttt ttgatgttaa agttggtgca gataaagaag gaaatttaaa agcaatagag
     4141 ctaaagtcat taaacaatac aggtgcttat ggtgggaatg gtgtctctgt ttcttccgaa
     4201 tctggacaca ataccttacc aatatataat gatattgatg ctgttagatt taatgctaga
     4261 acagtatata ctaataggtt accagcagga gcacttagag ggtatggtgc tacacaggga
     4321 atatttgcaa tagatagtgc tattgatgaa ttggcacata agattggaat ggatccactt
     4381 gaatttagaa ttaaaaatat aatagatagg tataccaatg gaaaagcagc tgaagacgaa
     4441 atattaagtt gtgagctgag acaatgtata gaaaagggca agaaattaat taattgggat
     4501 gataaatatc catgtaaaga tataggaaac aacaaagtaa gagcagtagg tatggcagta
     4561 gcaaatcatg gttctggtat accgcgtgta gatatggcaa cagtcactat aagaatggat
     4621 gaagatggca catataaact attatctggt cttgctgact taggtcaagg ggctgataca
     4681 attcaagctc aaattgctgc ggaagcttta aatactactg tagatagaat ttctatttat
     4741 acaggtgatt cagacttatg tccctatgat acaggagcat ttgcttcttg tacaacaacg
     4801 gttacaggaa atgcaacaat aaagacagca aataaattaa aagatatatt actttcagtt
     4861 gcagagtcaa agctaggatt taaaaaagaa gatttagagt tgggaaaaga cagggttttt
     4921 gttaaaaacg ataatagcaa gtttgttcta ttagaggaat tgggtagaga atctgttgga
     4981 ggaataggat ttgctagtga tgatgtacca gtagttttgg aagcaacaga atcttttggc
     5041 atgttgaaaa aaataaaacc atttatagca ggatttgcag agatagaatt agataaaaat
     5101 acaggcgaat ataaagtcat aaattttgca tgtgtacctg attgtggaac agttataaat
     5161 ccaactttag ctagaataca agtagaaggt ggtgtgatga tgggtatagg gtttgcaatg
     5221 tatgaagacc ctatatttga taaagatggt agattattaa cagatagttt cctacagtat
     5281 aaagttccta caaaaaaaga agttggaaat atattggtag attttgctga gaatttcgac
     5341 ccaggaggtc cttatggtgc taaatcatta ggagaaatag ttatacatac accagcacca
     5401 gctatagcaa atgcaatatt taatgctaca ggagttcgtt taagagagct tcctatgact
     5461 tttgaaaaag tttatatggg tatgaaaaat aataataaac aattataata aataataaga
     5521 tgtataaaag caagtaaatt taaattattt tgtaaaataa agtaattaaa tggacattat
     5581 atatggtgtt ttgtaaaaat accgtatata atgtctagta gagtatattc taaaggaggt
     5641 gagttcatgt caaacagaat aaaaaatata cactatggaa ctcagagaca acaattaaaa
     5701 aacttaacta aaaaagaata tcaagctcta cgagagatgt gcttcttagc taagaactta
     5761 tataatgttg gtacatataa tgtaagacag cattatttca atcaaggaga acatcttaac
     5821 tatgaagcta attatcatgt atgcaaagaa aatgagaact ataagcttct caatagtaat
     5881 atagcgcaac aaatattaaa agaagtagac agttcgttta ggtcattttt tggattaatt
     5941 aaattagcca aagaaggaaa atatgacttt agagggataa aatcacctaa atatttagat
     6001 aaagaaggat tttttagcat aataataggt caaataagaa tcaaagaaga tgggatttta
     6061 aatgtaccta tgtctccagc atttaaaaaa ctatatggga aagtaagtat aaaagttcca
     6121 caaaatatac tagagaaaaa aattaaagaa ataagaataa tacctaaaca taaagctagg
     6181 ttctttgaaa ttcagtattg ttatgaaata ccaaaatcag aggaagtttc aaatcaaaaa
     6241 aatgcactag ctatagactt gggtttagaa aatttatgta catgtgttac caatttaggt
     6301 gatagtttta tcatagatgg aaaaaggcta aaatctgtga atcagtgggc aaacaaacag
     6361 aatgataaaa tagaagcctt aaaattaaaa aagaatataa ctgcaataac aagaaaacaa
     6421 tttaaagttt ggaataagag aaataatcaa gttaaagact atgtaaataa aacctgtttt
     6481 tatataattg attattgtgt aaaaaataat attggaaatt taattgtagg atataatgct
     6541 aagttagaaa aaagtgataa tatggagaga aaaatatata gaaattttat aagaatccca
     6601 tttggagaga taaaaggaaa attagaatat ctgtgtaaaa taaaaaatat caattttatt
     6661 aggcaagaag aaagctatac tagtaaatca gatttttttg ctgatgattt tctacctgat
     6721 atcaacgtgg acaatattaa aaaatatgac tttaaagggg aaagaataac gcgtggacag
     6781 tacaaatcca gtgttggaat cattataaat gcagatgtaa atgcgagtct taatatacta
     6841 aaaaaatcac aagtagttaa tttatctaat ttacagaata atcctcaaat attaaagcaa
     6901 ccacagagag taagaatatc ttaattatat ttaaaagata aactgatagc gtaagcttac
     6961 agcttagcgg cgaattggat ccgccttgtt gtttacaaca gaatctattt actttcgagt
     7021 ttatggaatt tcaaaaatca cttttactaa aaaggtctaa ctataataat taagagatta
     7081 gaaaactggt tataagaggt gaaagtgtgg aactatcaaa tataatagaa aagaatgaaa
     7141 taataactgt agttggagca ggaggaaaaa cttcgtttat aaactatttt gcaaattttt
     7201 atagagataa attgaaagtt ctccttacaa caacaactaa aatatatgta ccaaacgatt
     7261 atgacaatat aattattaca atagatggaa ctgttattcc atctatttgt catggaataa
     7321 cagtatgtgg cagttatata aataatgaaa ataaattagt aagtattgat tcctctattt
     7381 tggatgaaat tgtagaccaa tttgatttag ttttaataga gggagatggc tcaaagagaa
     7441 aaaaacttaa aggttggaat gctaaagaac cagtagtata tcataaaact acaaaaacca
     7501 tagggatatt agatataaca tcatttggaa tgaatattaa tgaagagaat atacatagag
     7561 tagaaatatt taaaaaaata gcaaatttag atacaagtag tattaatagt agtcctacag
     7621 taagtattga aaacttaaaa aatatagtat taaatccaaa tggattattt aaaaattatt
     7681 ctggaaaaag agtattattt attaataaag ttgaaaatga aaaatataaa aatttagcta
     7741 taaaattaat agaaaatatt aaagaatgtg aaagtgaaat agagatattt tatggaagtg
     7801 taaaacaaaa attttgtgta agatattgaa gtattaaaat agtttatatg atattatata
     7861 tatagataat atcaatagag atattataaa aatcatagaa ctatcaatat attataaata
     7921 ccaaggatga tatatatgat aaatgcagtg ataatggcat caggctttgc cagaagaatg
     7981 ggagtaaata aacttttatt aaattataaa ggctgttcta taatagaaca tgtatttaga
     8041 gaagttagca agattgattt tcatcaagtt gtagtagtaa gtcaatatga agaggttttg
     8101 ttattgtgta ataaatatgg ttttgaatat atagataata aaaatgcaaa tataggtcaa
     8161 agtgaatcta taaaattagg agttttaaat agctcaaaat gtgatggtta tatgttcttt
     8221 gtaggagacc agccatttat tgataactta tatatagata aaattataaa tacttttaaa
     8281 ttagatagag attttatagt aattccaaga tacaaagata tgtgtggaaa tccagtcata
     8341 ttcccatata gtaagaaaga agaattgttg gcacttaaag aagatgaaaa aggtaaaacc
     8401 attttaaaaa attcatcaaa aataaagtat gtagaagttc ctaaagaaat gttatttgat
     8461 atcgatacaa aaatggatta tgagagaatt gggggtaaat tatgaagcta ataaaaacta
     8521 tagatgctgt tggacaagtt ttatgtcacg acataactca aataatacct ggagaattta
     8581 aaggaagaaa atttaaaaag ggccacatag taaaagaaga agatattcca gtgttattat
     8641 ctcttggtaa agataatttg tatgtatggg aaaagtcaga aggaatggtt catgaaaatg
     8701 aaggagcatt gtttttaaaa gaactaactg ctggagaaaa tttagatttt agtgaaataa
     8761 aagaagggaa aatagatttt atagcagcat gtgatggtct attaaagata gatgttgatg
     8821 cactttttga tttgaattgt gttggagaaa taatgatggc tactcttcat aataatttta
     8881 tagtaaataa aggtttaaaa gtagctggaa caagagtgat acctttagtt atagatgaga
     8941 aaaaattaga agatgcaaaa aaagtagttg gtaatagaaa aatagtaaat gtagtacctt
     9001 ttaaacctaa gaaagtagga attgttacta caggaaatga agtgttttat agcagaattg
     9061 ttgataaatt tggaccagta atagaagaaa aagtaaaagg ctttggttgt gaagttatag
     9121 gacagactat atgcccagat gataaagaga ttataaagag tgcaataaaa gaatttataa
     9181 gtcaaggagc agaacttatc tgttgtactg gtgggatgag tgtagaccca gatgatgtta
     9241 cgccaacagc tataaaagaa actggagctg atttagtgac atatggttca ccaatattac
     9301 caggagctat gtttttactt gcttattatg gagaagttcc aattatgggt ataccaggat
     9361 gcgcaatgta tcacaaaact acagtatttg atatagtttt atctagggtt ttaattgatg
     9421 aaaagcttga taagtatgat atagctagat atggtcatgg tggcctttgt atgaactgtg
     9481 atgtatgtac ttatcctgca tgtaattttg ctaaaatata aagttattaa aaaagagtat
     9541 ttacaaatta agtactcttt ttaaatatga aacaaaacag ctagaatgat gttttaaaaa
     9601 aagcatattt atttagaaat tataataact tattttcaat gtgtaaaaga atgaataaag
     9661 ttgcaatttg atttctttat aagtataata taagagattg ttaattttta taaatttaca
     9721 atttttagta ttattttaat aaatatttca caaatttagt ataaaaaata tactatttac
     9781 gtgataatat ataataaaaa tgttatcaaa gttttatgat aaaataaatt atgagcaaaa
     9841 agaaaggaaa atatattaat gttacaagtt acaggtgttg gacttagatt tggtgataag
     9901 gaactattta aagatgttaa cttaaaattc actaaaggta attgctatgg aataatagga
     9961 gctaatggtg cgggaaaatc tactttttta aaaatattat caggagaaat agagcctaat
    10021 acaggtagta tatctataac tgataaagaa agaatgtcag ttttaaaaca ggaccacttt
    10081 gaatatgaag aagaaactgt tttaaatgtt gttataagag gtcatgagag actttggaac
    10141 ataatgaaag aaaaagatgc attgtatatg aaggaagact ttaatgaaga agatgggata
    10201 aaagctgccg aacttgaagg tgagtttgca gagcttgacg gatgggatgc agaaactaat
    10261 gctgagaaaa tacttatggg tcttggcata acgaaagata tgcattacaa acaaatgaag
    10321 gaactagttg gtggggaaaa ggttaaggta ttgttagcac aatcactttt tggtaagcct
    10381 gaaatactat taatggacga acctacaaac cacttagatt ttaaatcaat aaactggtta
    10441 aataatttta taatggattt agaagaatct atagttataa tagtatcaca tgatagacat
    10501 ttcctaaatc aaatatgtac taatatagtt gatgtagatt ttggtaaaat acaaatgtac
    10561 gttggaaact atgatttctg gtatgagtca agtcaattgg ctttacaact ggctaaggac
    10621 caaaataaaa agactgaaga aaagatagct caattaaagg aatttatagc tagatttagt
    10681 tctaatgctt caaaggctaa acaagcaaca tcaagaaaga aacagttaga gaaattagaa
    10741 gttgaagata tacaaccatc aagaagaaga tatccatatg taggatttac acctgcaaga
    10801 gaaattggga atgaagtttt agaagtacac aatttaacta aaactataga tggggttaag
    10861 gttcttgata atgtgtcatt tagattggat agagatgata aagtagtatt tatgggtgat
    10921 gaaatagcta ctactgcact tttcaacata gttatgggag aacttgagcc agacagtgga
    10981 gagtataaat ggggagtaac tacttctcaa gattatcttc caaaaaatca taataagttt
    11041 tttgacggag tagaatattc tttggttgac tggcttagac agttctctga agaaaaaagt
    11101 gaaagtttta taagaggatt tttaggtaga atgttattct caggtgaaga agcattaaaa
    11161 gaagctcaag ttatatctgg aggagaaaag gttagatgta tgcttagtaa gctaatgctt
    11221 tcaaatgcaa atgtacttgt attagatgac cctactaacc acttagacct agaaagtata
    11281 acttctgtaa ataaaggact tgaaaagttc ccaggagtgc ttttatttac ttctcatgac
    11341 catgaattta tatctactat agcaaataga attatagaga taactccaaa tggaataatg
    11401 gatagaaaaa tggactttga tgagtattta gaaagtaaag agatacaaga ccaattagct
    11461 aaaatgtatg gaaaagataa ataattatta gtataaaacc ttttaatatt tgagacatgc
    11521 tatgcatgag tgtgtaaatt tttaatcaaa tattaggagg ttttttatgt tgtttaaaca
    11581 tgtttattag cagaatagag taatattttg tatattaaga atttaaatat aatatagtag
    11641 atgatataat aaaaatatat aacattttgg ggggaataga ttatggttaa gaaaaggtta
    11701 gtagctgtag tattgagttt agctatgatt agtgtagggg atttttcata tgctcactca
    11761 ggagggcata attcatcagg aggtcatcat aataaaaata aatcagagtt aacacattat
    11821 tattgtggag gacattcagc acatcaacac aataaaggtt tttgtcctta tgctagtaca
    11881 aaagaaaatt ctggagtaac aatatcttca gtacataacg aaaaaaatca taaaaactat
    11941 tttgcagaaa aagggtataa taaaggttat gaagatgggt gtaaagggct ttataattta
    12001 agtgtcacag aagccaaatc tgataattta tatgaaaaag agattttaaa aagttcatat
    12061 gaaaaaggat atttaattgg atacaatgaa tataaagcaa aaaaagtaga tgatgtaatg
    12121 aagaagatgt tagatattcc aatgttagca agtatataat aatatagtat ttaaaaagat
    12181 attttctata agggaatatc ttttttattg taaagtataa tttttcgtaa aaaagtataa
    12241 taactgaggc gataaattaa aataaaacag attatttaag tagttattaa tttgagatag
    12301 atacatttaa tattaatgaa tctatctcaa taagctaagc taatttaaag ctaagataaa
    12361 ttaatattaa ttagcattaa ataaactttc agaaattttg tcagaagcct ctttatccat
    12421 ttctttaagt acatgagaat aaacgcttag ggtagtactt atatttgagt gtcctactct
    12481 ctcggatata acctttatag gtactttaga atttataagt aaagtaacat gtgagtgtct
    12541 taaatcatgg aatctaatat gaggtaattc attcttttct aaaaatcttt taaatttttt
    12601 actcataaca tcttcagcaa taggttcaca tttcttattg aaaaacaaca aattattatt
    12661 atttctaatg gaagatttta ataaattttt attctgtttt attttatatt cttttaataa
    12721 agacattaat tctttgggag aagatatttt tctaacagag ctttctgtct ttggctcttt
    12781 gagaataaca gaaccattaa ttcgggaagt tatcttattt actgttattg tattttcttc
    12841 aaaatcaata ttatcccaag ttagacctaa aacttctgaa attctaagac ctaatccaat
    12901 agccaaatta ataggtaatt ccatttctgt attttttgct aaatctaata attttatcat
    12961 ttgttctcta tcataaatct catttttaaa ttttttatat cttggagcct ctatactatc
    13021 cataacattt tcttttatta atttgagtct ataagctctt tttattgcaa gtttcaatat
    13081 atttatatga atcttaattg attgaggatt tagtttataa gatagtttat ctatgtagtt
    13141 ttgtatgtga accggatgta agtcttgtat tttatatttt ccaatagatg gattaatgta
    13201 attcttacaa atactaacat aattattata tgtagctaaa gatatatttt cactatattt
    13261 atttagaaaa tctaatagaa attctgacac tgtaatttcg cttggtacta aaaaaccatc
    13321 attatataag ctttctttta cctcatttag tcttttactt gcttcccttt ttttatcaaa
    13381 agtacccata tttttttgtt ttcttttacc tgtcttttca tcttcaaatt ccaggtaaac
    13441 aacatagttt ttatttcttt ttctaataaa tgcattcata tataacccac caatcaaaat
    13501 tttatac
//
LOCUS       DAEORZ010000114        12838 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEORZ010000114 DAEORZ010000000
VERSION     DAEORZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.115"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     regulatory      68..170
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            264..1640
                     /locus_tag="KRH39_002840"
     CDS             264..1640
                     /locus_tag="KRH39_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG8512728.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1754..2899)
                     /locus_tag="KRH39_002841"
     CDS             complement(1754..2899)
                     /locus_tag="KRH39_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG8512729.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITSKTLQNGELIDIIK"
     gene            3152..3859
                     /locus_tag="KRH39_002842"
     CDS             3152..3859
                     /locus_tag="KRH39_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512730.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3906..5501
                     /locus_tag="KRH39_002843"
     CDS             3906..5501
                     /locus_tag="KRH39_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512731.1"
                     /translation="MSNLSILIKNNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINVK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKVLMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNVYIILASIL
                     TLILDFMIWRIISTKGVKIFKNL"
     gene            5604..6686
                     /locus_tag="KRH39_002844"
     CDS             5604..6686
                     /locus_tag="KRH39_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG8512732.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            6835..6996
                     /locus_tag="KRH39_002845"
     CDS             6835..6996
                     /locus_tag="KRH39_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBG8512733.1"
                     /translation="MKQDKKNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGDRI
                     DKKKMNRPS"
     gene            7337..8605
                     /locus_tag="KRH39_002846"
     CDS             7337..8605
                     /locus_tag="KRH39_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG8512734.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            8800..9588
                     /locus_tag="KRH39_002847"
     CDS             8800..9588
                     /locus_tag="KRH39_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG8512735.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            9588..10037
                     /locus_tag="KRH39_002848"
     CDS             9588..10037
                     /locus_tag="KRH39_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG8512736.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            10041..12305
                     /locus_tag="KRH39_002849"
     CDS             10041..12305
                     /locus_tag="KRH39_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG8512737.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVK
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGRMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            12445..12756
                     /locus_tag="KRH39_002850"
     CDS             12445..12756
                     /locus_tag="KRH39_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512738.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         4699 a         1398 c         2196 g         4545 t
ORIGIN      
        1 aatacaatat atacgtatat attgacaaaa ttaattatat aaattaaaat aatattcgta
       61 aagaaaaatt catgttttgt actgtataac atataatttt gacaatatgg gtcataagtt
      121 tctaccggaa taccgtaaat attctgacta tgtatactat tatttagtta attaaatatt
      181 taattaactg aattatgaag gatacggctc agcttatcaa ctactgtgtc gtttttttgt
      241 aaaatttaat aggaggtgtt gttatgcaaa caacaaacag tagtactatg cagaaacttt
      301 ttccaatttt gactaataaa aatgtcgaca tgaagaagga aataattgca ggagtaacta
      361 cttttttaac tatggcttat ataatagctg taaatccaaa tattttatct gagacaggca
      421 tgcctgcagg tgcacttgtt acaggtactt gtttagcagc agcatttgga tgtattttaa
      481 tgggtgtagt tgctaacctt ccttttgctt tagcatcagg tatgggtctt aatgcatttt
      541 ttgcatatac agtagtttta caaatgggtg ttccttggga agtagctctt acagcagtat
      601 ttgtagaagg tattattttt atagtattat cagtaagtgg agttcgtgag gctgttgtaa
      661 atgcgatacc aaaaaatatg aaattagctg ttacaggtgg tataggaata tttattgcac
      721 ttataggtct agttaacagt ggaattgtta ttggagacca agccactctt atcaaaatgg
      781 gtagatttac accagcagta ataataactt gtgtaggatt aataattatt gctgtattag
      841 ataaaaaaag agttaaaggt tctatacttt ttggaatagt agtgagttca ttacttgctt
      901 gggcatttgc ttttataaat ccagagtatg cacaaaaatt aggtatatat ttacctggtg
      961 gattatttaa attcgaaagt ttagcaccaa tagcaggtaa aatagattta ggatatgtac
     1021 tacatccaac aaatataggt ggttttttag tagtagtttg tacattttta tttgttgatt
     1081 tttttgatac agtaggtaca ttagttggtg tttgttctaa agcaaacatg ttagatgaaa
     1141 aagggaatgt tcctaatgta ggtagagcct tacttacaga tgcggttgca actacagttg
     1201 gtgcaggact tggagtatct acagttacaa cttatgttga gagttcaaca ggagttatcg
     1261 caggtggaag aactggatgg acagcaataa cagttggaat tttattctta gctgcaatgt
     1321 ttttctctcc tgtatttata gcaattccat catgtgctac agcaccagct ttaatatatg
     1381 ttggatattt aatgcttggt acagttaagg atatagagtt tgataatata actgaaggtg
     1441 ttccagcttt tgttactatt gcttgtatgg cattaacata tagtataggt gatggtttaa
     1501 ctttaggtat attaacttat gttttcgtaa atatatttta taatatattt ggagctaaaa
     1561 aggcagaaga caaaaaacat gtttctgtag taatgattgt tctggcaata ttatttataa
     1621 taaaactttg gctactataa taggtattat ttaaaatttt aatttaatat aaaataaagg
     1681 gctgtaattt attatcaatt gtgatgataa actacagctc ttttgtattt gttgtatttt
     1741 tgtatttatt gtattattta ataatgtcta ttagttctcc attttgtaaa gttttagatg
     1801 taattactat attgaactct ttttgcaaat cttttataat atctaattct ttttttgtta
     1861 ttattgagat tgtgttacca cttcgatttc ctctagcagt tcgaccacat ctatgcaggt
     1921 attcattttt actttttgga aaatccaagt taaaaacatg acttacatcc acaatatcta
     1981 gtccacgagc agataaatca gttgtaatta aaatctttgc tttaccaagt ttaaatttat
     2041 ttattgcatt ctttctatct tctttttcca tatttccaaa aattcctatt gctttgtaat
     2101 tatgataatt tagttttgat actaggactt ctatattttt ttcattattt acaaatacaa
     2161 tagctttttt tggatttgta gctgctaatg cctttctaag aaaagtaaat ttatccctta
     2221 tctcgcctaa taaatatgag tgattgatgt taggatttat ttgtgatttt tcctttgttt
     2281 ttattatttc aaactccttc ataagtttat cacaaatttt tatagtgcta tcagtaagac
     2341 ttgcagagca tcctataatt tgtctatctc ttaacgttgt tcttattatg tcttctatac
     2401 aagttatatt ttttccattc aatagattgt ccacttcatc taagacaata gtttttatat
     2461 tatgtgattt tagtttcttt tgttttatca agtctaaaac tcttccacaa gagccaatta
     2521 ctatgtgtgg ttttactgct tttatatttt ttatttgttt ttgtatattg acctccccaa
     2581 ttaaagctaa agaagtaacg gagagttctg catttttagc caataattca acctggtttg
     2641 taatctgcat tactaattca tgcgtaggtg caagtataag agcttgtgtt tctctttttg
     2701 atgtatcaat tttttcaaat ataggtaaaa ggtacgctag tgtttttcct gttccagtct
     2761 gtgaattaat caataaatcc ttattttgaa ttatatttcc tataacaagt gactgaactt
     2821 ctgttggaga agttatgtct tgttttttga gtccatctat tagagtggaa cttattttta
     2881 gttgttcaaa tgtattcata ttttgctcct tatattttaa taattatagt caactatttt
     2941 ttattataca ttgtttattc aaatcgaact atatattctt ttataattat gtgtaatttg
     3001 aaagtaaata tcaattatat ttataccttt atatttctga tacctttgca ttttatagat
     3061 ttctaaaata aacaaataaa agttgtaatt gtttgatatt tgttatatat tatatataac
     3121 aaatatcaaa actataatat ggagggaaat tatgttaaaa gtaatcaatg tatctaaatc
     3181 atataaaaat aaaaaagtag taagtagtgt ctcatttgat gtaaatgatg gagagctttt
     3241 tgggtttata ggacataatg gagcaggtaa aactactact attaaagcaa ttgttggaat
     3301 acacgatttt gaagagggag aagtactaat aaattctaaa tctataaaaa aacagcctgt
     3361 tgaatgtaaa aaggaaatgg catatatacc tgataatcca gatttatacg agtcattaac
     3421 tggtatacaa taccttaatt ttatagctga catatttgaa gttgccaaag ataaaagaga
     3481 agagttaata aaatattatt caaataagtt tgaactcaat aaagcattag gagatttaat
     3541 atcttcatat tcgcatggta tgaaacaaaa attagctata atttcggctt tgatacacag
     3601 cccaaaaatc ttaattcttg atgaaccttt tgtaggtctt gacccaaaag cttcctttat
     3661 attgaaggaa attatgaggg agttttgtga caaaggtggt tgtatatttt tctcaacaca
     3721 cgttttagaa gttgctgaaa aaatttgtga taagattgcc ataataaaag atggaaatat
     3781 aattgcttat ggaactacag aagaagttaa gggaagtaat tcacttgaaa gtatctttat
     3841 ggaattaata gagaaataaa gtgtttttta tacaatatag tgtctaaagt gaggaatgat
     3901 aaaatatgag caatctaagt atattaataa aaaataatat aataaatgaa tttaaattaa
     3961 atgcgttaaa aaaagctgat ggtagagaaa aacaaaaaat aatgggaatg atgctttcag
     4021 ttgttataat agcgggtcta ttaatttttt atgtaacgag cttaagtctc tctttagttg
     4081 atatcttaaa gcaaataaat caactggaaa tgctactgat atttggtttt ggattatcaa
     4141 cattaacaac tattgttaca tcattatata aaacgtcatc atatctattt caagcaaaag
     4201 atttagacct tttgactagt ttacctataa aagaatctac tgtactagca agtaagatat
     4261 taatgttggt tggagcaaac tatttatttt cttcaatatg tatgattata cctgcagtgg
     4321 tttacttttt aaattctgat gtaagtataa cttatttccc ttatttgata ttgctatttt
     4381 tagctttacc attattaccg atagttgctt catcaataat ttttctattt atagggcaga
     4441 tatcatcaag aataaaatac aaaaatttag tattaatcat aggtagcatt atattgatgt
     4501 tgctttatac cctacttatg tcaagactta attctatgtc agttgaaatg ctaaagaata
     4561 gtgtgtctat ttcagagacg attagaaaag tatatcctcc tatatattat tttgttgatg
     4621 ctcttaaaac aggaagtata gtttcatttt tagcatttat agccatctca ataataccat
     4681 tttttatgtt tgtaactctg tttgcaaaaa aatttaaaga tataaatgtt aagatgagtg
     4741 aaggatataa gtcaaaaaat tatgaattta agggtcaaaa aacttcaaga ccttcaaaag
     4801 ctttattaaa aaaagaattt aagagatata cttcttcgtt tatatacttt ttaaattcat
     4861 ctgcaggttt gttatttcta atagtaggta caattttaat attattattt ggagcagata
     4921 aagtgggtga gttactgaaa ttgaatatag attttaatct tataaaaata caattattgg
     4981 gagtattctt ctttataata gtaatgaatt gtacaacata ctgttcaatt tctcttgaag
     5041 gaaaaaactt atggatactg aaatcttctc caatagatga aagagaaata ttctggtcaa
     5101 aagtattaat gaatttttta ttgaatgcac cattatctat tttatgtcta atattagtat
     5161 ctataagatt agactttgaa attcagttta ttattgtatc aatctgtttg attgtaagtt
     5221 tttcaatgtt tgtagcattg atgggattat tatcaaattt attataccca aaccttgatt
     5281 ggaaaaatga ggtggctgta gtaaaaagaa gtgcaagtat gatagttaca atgttagtat
     5341 cattagctta tgtaattata cttggaggta tatatgtatt acttggaatt tcgtttttaa
     5401 atgtatatat tattttggca agtatactta ctttgatatt agattttatg atatggagaa
     5461 ttatttctac taaaggagta aaaatattta aaaatttata ggtgtattta tgtcaaataa
     5521 agacttatct tttttagtct tgcaaataat aggttaagta gtatactatg tatagtgata
     5581 tgaaaaaatt taggagaaaa taaatgagaa aattatccaa aagagaaaaa gattttattc
     5641 aatatggatt tttattattt ttgatatgta ttactactta tttagtatta actactttag
     5701 atataaaact aatacctaaa attataaggc tagtaaataa gacttatata tttcttggca
     5761 tattgacaat tatagtatat atgataattg aatctattgt aactcatttg ataataaatg
     5821 cagttcaaaa aacaaaagta aaatttattg gtattaaaat ggctacaatg ggattttact
     5881 ataacttagt tactccattt gcatctggga gtcaaccaat gcaaatatat gcacttacaa
     5941 aatatgatgt aagccttagt aaatcagtgg ctatagttac taataaaact gtagtatttc
     6001 aaagtacagt tactgtattt tgtggaattt taattttgct aaataagtca ttactgatgg
     6061 agcaagtaca ttctgtgaaa gtcttgataa cttttggaat gattatgaat gtaagtatgt
     6121 tgttaggtgg actactaata gtatttagtc caaaacaagt aaaaatgata gcagaagttt
     6181 tgattaattt tttatctaaa tttaaaacat ttaaattttt agattcaaaa cgtgatagaa
     6241 taaatcatta tattgatgat tataattatt ctataaaaat atttattaaa aatgtaagaa
     6301 tcttagtttt aagtatatta cttactgtgt tacaattaat cgcatatttc agtatagttt
     6361 attgtgtgta taaagcctca aatttaagag gagtttcata tgtacatata atgacattac
     6421 aggtcttttt atatatggct atatcaccaa ttccaacacc aggaaatgtt ggagctaatg
     6481 aggttacatt ttttactatg ttctccaata taattccaaa agaattattg gggtactcgg
     6541 tactcttata tagtgggttt gtatactatt ttatattaat agtttcaggt ttatttacat
     6601 ttggaacgca ttatacatta aattcttgga aaaaggaaga taaagtaaat tctaatttag
     6661 ataaggttat tgaaaaatta aattaataaa ataattaggg aaattctata tataaatatg
     6721 gtgttgagta agctatatta tattgagata tttccctttt ttgattaaaa ttatagaaat
     6781 ttgaaataat aagtaataaa cagaaagagt gatattgttt tgaaaggaga aactatgaaa
     6841 caagataaaa aaaatgaaaa acaaagtgag atttctaaga taaaacaaag caatgataat
     6901 aaacttgaaa tagctcaaga attacatgta agtaaaaata tagggaatat gcttggagat
     6961 agaatagata agaaaaaaat gaatagacca agttaatgaa aatgttgaaa atggtacatt
     7021 ttaaaaaata tatataatga aatgttttat tgttaaattt aaacttattt taatggagga
     7081 aaaagtcgaa aaatatctaa aaagatatgg atataaaaaa tattaatata tatatactat
     7141 aaacttgaca aaatcaacct tttaataact agcaaaaaat agcaaaatta tataagcgtt
     7201 atttaattgt caaaaatgaa agctaaattg catgtcctaa aaaaaatgat tgataacatt
     7261 tatttgtgtg ctataattat atagtaaaac aataagtata ttgcataatg tatgcaaatc
     7321 gggaggagag acggacatgg aagcatctat acacacaatt gccagcaata atttattgat
     7381 aatttttgct attgtaagta taactggaat aatttgtagt aagcttagtg aaataataaa
     7441 ggttccagat gtagtgttat atttacttgt aggtatttta ataggaccat catttttaaa
     7501 atttatagat attcgtggat ttcaaataga aaatcaacta atacttacat ttggttctgc
     7561 atttatttta tatttaggtg gaaaagaaat aagtttaaaa gtattgagaa atgtgaagat
     7621 atctgtattc ttattagcta ctttaggagt tgtgatatct gcttttataa tgcaacaagt
     7681 aataggattt acttttggga taagtgcaat gactgcttta ctagcaggaa ctataatagc
     7741 atcaacagac ccagccactc ttgtacctat atttaatcag gtaaagataa aggatagggt
     7801 aaaacaaact gtaataagtg aatcagcttt taatgatgca acaggagcta tactaacatc
     7861 agcagtgttg gctgtaattt tgtcaaataa attttcatta ggtgaaaata tttatgaact
     7921 ggggacaatg gttattgtcg gagttttagt tggtttaatt acaggaatac tgcttttgaa
     7981 attagttaat gataaacctt atggaatatt taaagatttt gcaccaataa tatctgttat
     8041 ttcagttgta gttgcatatg aattatctac aaaattaggt ggaagtggat atatgtcttg
     8101 ctttattgta ggtataataa ctggtaacaa gaaaaatttt aaaatttggt taagtcaaaa
     8161 atcttatgat gcagattttt atgttgctga aactttaggt actatttgtc gtatggcaat
     8221 atttataata ttgggtagcc aagttaattt ggctcagtta agtaaatatt ttatgccatc
     8281 tgtaattaca gtattagtat taatgtttat agcaagacca ctttgcgttt tagtttgtac
     8341 acttgtagac agggaagcaa aatggagtaa aagtgaaata ttatttatga tgtgggtaag
     8401 ggaaacaggc gtaataccag cagcactttg tggtattatt tcagcaatga aagttcctgg
     8461 atacgaagtg atttcatctg tagtattcat gactatacta attacattag taatacaagg
     8521 aagtactaca aaattagtag ctaaaaaact tggattatta gaagaagaag tgataaatat
     8581 aagtgaaaaa gtatcaactt tttaaagttt tgctttaaaa tagtacctgt taatggctat
     8641 tttaatattg tctaatgatg attattgaaa tagtcatttt tttatgtata agtaatttac
     8701 agaatttaca tattagacat atttattatc aaaatttgtt gaagctatga tataattagt
     8761 tgtatttaaa ataagaatag caaataaggg aggaaattta tgtttacggt tatggatatt
     8821 gtacagccag atacagtaga agaagcgtac tcaattttaa ataaaagaaa aactaaccaa
     8881 gtaattggtg gaagtgcttt tttaagaatg gggaaaaaaa gaataggaac aggaatagaa
     8941 ttatctaaat taaatttaga ttatataaaa gagtatgaag attacgttga gattggttct
     9001 atgactactt ttagaacatt agaaacaagt tccataataa aaaataattt tggaagaata
     9061 atagaagatt cagttaaaaa tataatagga gttcagttta gaaatgtagt aacagttggt
     9121 gcaacagtat tttctaaata tggtttttct gacttgatag tagcattact ttcattagat
     9181 acagaagtag aattatataa tattgggaga attagtcttg aagagtttct aaatagagat
     9241 tatgagaaag atttacttat taagatatac ataaagaaga caaataagaa tgcttcttat
     9301 aaatcactta gaaatgcaaa gagtgattat ccaattctaa atgtatctgt atcaaaacat
     9361 atggaacaat ttaagttatg tgtaggagca agaccgcaga aagctacgat tgcaaaacaa
     9421 gctagtgaat ttctatctaa caatgaaatc aatgaaatta atatagataa agcagtagaa
     9481 atagcatcag aagaactaac ttttggttct aacatgagag catctaggga gtatagaaaa
     9541 gctatgagta aagtactttt aaaaagagct ataatggagg ttatttaatg ttagtaaaga
     9601 ttaatataaa tgggaagaaa aaaagtgttg atatagaacc agaagaatat ctggttgata
     9661 ctttaagaaa agttggtaat ttaagtgtga aaagaggttg tgacacaggc tgttgtggtt
     9721 tatgtacagt tttaattgac aagaaaccta cattatcctg tgcaacactg acattgagag
     9781 ctttagataa agaaataact acaattgaag gtttagaaaa tgaagttaaa gaatttagtg
     9841 aagttttggt aaaagaagga gctgagcaat gtggtttctg ttctcctggc tttatactta
     9901 ctgtacttgc aatgaaggat gagcttgaaa atcctacaga tgatgaaata aagcattatc
     9961 ttacaggaaa tctatgtaga tgtacagggt atatgggaca gttaagagct atcaaaactt
    10021 atctggaggt aaaatagaac atgaatatag ttggaaaagg aatcaagaaa atagatggaa
    10081 tgagtatcac aacaggaaag cctgtatata cagatgattt atcagcaaaa gatgctttgg
    10141 tggtaaagat acttagaagt cctcacgctt atgcaaaaat aaaagatata gatacatcaa
    10201 aggcagaaat ggttgatggt gttgaatgtg ttttaacata taaagatgta ccaaaatctc
    10261 gatttacact tgctggtcaa acatatccag agccatctcc atatgataga ttaattcttg
    10321 aagatgttgt aagatatgta ggtgacgaag tagctatagt ggcagctata gatgaaaaaa
    10381 ctgcaattaa agcaatgaat cttatcaaag taaagtatga agtacttaca cctgttttgg
    10441 attttgaaga ggcaatagga aataaaacta tggtacatga tgaagataga cattgtaact
    10501 ttgatatagg tatgcaaaga gaaaaaaata tagtatccca tcatgattat acaaaaggtg
    10561 atgtagaaaa agtattaagt aattgtgatg tagtgattga agaaacttat tatactcaag
    10621 ctcaagcaca tgcaatgatg gagacgtata gggcatacaa ttatttagac catatgggaa
    10681 gattggttgt agtaagttca actcaaatac catttcatgt aaaaagacat ttatctcgtg
    10741 cactagaaat accagctagc aaaattaggg ttataaaacc tagaattggt ggcggatttg
    10801 gtggtaaaca aactgcatgt gttgagattt tttctgcaat tgttacttta aaaacaggga
    10861 aaccagctaa aataatttat gatagaaaag aaacttttaa ttgttcaaca agtagacatg
    10921 caatgagatt aaaagttaga ctaggtgcta caaatgatgg cataataaag gcaatagata
    10981 taaaagcact ttctgataca ggagcatatg gagagcatgc ttctacaact attggtttgg
    11041 ttggagaaaa aactttacca atgtataata aagttgaagc aacaagattt attgcagatg
    11101 tggtatatac taataagatg ccagcaggag cacttagagg gtatggagca tcacaaggat
    11161 gttttgctgt tgaatcaact ataaatatgc ttgctaataa attaaaaatg gacccaacag
    11221 atttaaggtt aaaaaatgtg gtaacagaag gtgaaactac atttgcatat ggtaaaactt
    11281 taaatagtgt cgatttaata aaatgtataa ataaaggcaa agaacttatt ggatggaatg
    11341 aaaaatatcc ttgtaaacat cttgataatg gaaaaattag aagtgtgggc atggcaatta
    11401 ctatgcaagg ttctggtata caaggtcttg ataagtcttc agctgaaata aaattgaatg
    11461 actgtggaga ttatactctt atgataggtt ctactgatat gggtacagga agtgatacga
    11521 ttttagctca aatggctgct gaggttcttg atacaagcat tgaaaatata acagttatat
    11581 cagctgatac tgacttagtt ccatatgacc caggttcata tgcttcaagt actacttatg
    11641 taacaggtat ggcagtagtt aaggcagctc aagacttaag agaaaaaata atttctgctg
    11701 gagcaactaa actaggtgtt gagaaggatg aagtaaaatt tgatggtata aaattaacta
    11761 gtaaaaaagg tagcacttta ttattcgata tagcagttga ttctacaatt ggtccagata
    11821 ctaatcaatt aataggtctt gcttctcatg gtagcccagt atctcctcct ccttttatag
    11881 caggttttgc agaaactgaa attgacacag aaactggaaa agttgattta gtaaattatg
    11941 tagctgtagt agattgtgga acggttataa ataaaaatct agctaagata caagttgaag
    12001 gtggaattgt tcaagggatt ggaatggcat tatttgaaga agttagatac acagaaaaag
    12061 gaagaatgga cacaaataca tttatgcaat acaagattcc agctagatgt gatattggaa
    12121 atgttaaagt agattttgtt gaaacttatg aaccaactgg accatttggt gctaaatctg
    12181 ttggagaagt tgttataaat acacctttac cagcaataca agaagctgtg tttaatggaa
    12241 ttggtgtaag aataaataca ctccctatta cacctgaaaa agtttttatg gaaatgaata
    12301 aataattatt tatgaaaatt tagactagtt ttacatgtta aacaaccagt tatgtcatac
    12361 ctgttgaaag atatttaata agtagttatt ataatattat gaattttact tataaaatac
    12421 aaaattaaat aaggggatga tgttttggct ggtaatctaa ataatatgag agcagtaaat
    12481 aattttagag gagataagaa cattttagaa tgtttagtca gctttgaggg tcgttcaata
    12541 agtcagagaa aagtaagggt attttttaaa gaaaaacaaa atcaaataga aattgatttt
    12601 gcagaagagg aaatttctaa attggttgaa aatgttgttt taaatacatc atatcaagaa
    12661 atgttatatg atgaaataga gaaacaactg gaaattgatt gtataggtac ttggatgata
    12721 ttatctaaat taaaagatgg tagtagagtt cactgatttt aaaataaatt attagagcct
    12781 aattttaagg ctcttttttg ctgtttttaa ttaacaatag aaaatttatc tttagttt
//
LOCUS       DAEORZ010000115        12795 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEORZ010000115 DAEORZ010000000
VERSION     DAEORZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12795
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.116"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            766..1974
                     /locus_tag="KRH39_002851"
     CDS             766..1974
                     /locus_tag="KRH39_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512739.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLI
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            2005..3384
                     /locus_tag="KRH39_002852"
     CDS             2005..3384
                     /locus_tag="KRH39_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8512740.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            3490..4680
                     /locus_tag="KRH39_002853"
     CDS             3490..4680
                     /locus_tag="KRH39_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8512741.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNIPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(4808..6136)
                     /locus_tag="KRH39_002854"
     CDS             complement(4808..6136)
                     /locus_tag="KRH39_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512742.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(6754..7968)
                     /locus_tag="KRH39_002855"
     CDS             complement(6754..7968)
                     /locus_tag="KRH39_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG8512743.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITNEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            8394..9110
                     /locus_tag="KRH39_002856"
     CDS             8394..9110
                     /locus_tag="KRH39_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBG8512744.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRSDNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            9245..9871
                     /locus_tag="KRH39_002857"
     CDS             9245..9871
                     /locus_tag="KRH39_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG8512745.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(10084..11628)
                     /locus_tag="KRH39_002858"
     CDS             complement(10084..11628)
                     /locus_tag="KRH39_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8512746.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(11654..12295)
                     /locus_tag="KRH39_002859"
     CDS             complement(11654..12295)
                     /locus_tag="KRH39_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBG8512747.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            12565..12756
                     /locus_tag="KRH39_002860"
                     /pseudo
     CDS             12565..12756
                     /locus_tag="KRH39_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
BASE COUNT         4664 a         1565 c         2011 g         4555 t
ORIGIN      
        1 gtcagaatat attgacaaag aaaagattta aaattataat ctatattaag aatagtttga
       61 caatataaaa acaagtattt ttataaatag tgaaattatt tttattgtca atttaagaat
      121 aatttaggac tactataaag ataaaggtat agatgtcatt atatgataaa ttatacttgt
      181 atagaaagaa ttttaaagta tggcttatca ttgatttaat ataataaata atttttgttt
      241 ttagtaatag tctctaggaa taagatatct aaattcgctt agaaatagtc taatttacaa
      301 gggaggggat gcactaatag ataaaatttc tatataaaga agacttaaag tgaagtatat
      361 tctgataata ttggtttaat gggtatatta ttatttcgtt ttagatattt taagaataat
      421 ttaagacgtt tataaaaata tattctatat ctaatattta aataaatata agtcttccaa
      481 catgtatact aatatagaga atagttgaag tacaattcta actcgtaaaa ttatgtagaa
      541 agtaaaaatc aaaaagaaat ctagtaagtt gataactata gttaatattt agagagttaa
      601 ctctagttat atttatttag atattttagg aataatttag gacgtatata atatcatcaa
      661 aaaatatcaa gctataccat atgtagttaa gaataactgc ataatataca ttttataatt
      721 attgttttat tgataaaaaa ttattatatt taggaggaaa caacaatgga aaatttttgg
      781 aatccactta cttgctttac tgtatgtgta atgatttatg cttttagtga gtttttatct
      841 aagaaaacaa aaggtgcaat ttcaacattg ttatttgtat gtattatatt tttaatagga
      901 ttttggacaa atattttacc aaaagacatt acaggagcat cagggatatt agctgcaatg
      961 agcaactttg gtatagcttt attaataact aacttaggaa cacttataaa tcttgaagac
     1021 ttatgtcatg aatggaaaac ggttgtaatt gcattagtag gtattgtagg aataggggtt
     1081 ggctgtttaa cagtgggtag tatgttattt ggtagagagt atgctttaat agcagcacct
     1141 ccaatagcgg gttctactgt tgctggtatt atcgtaacaa ctgttgcaga agctgcaaat
     1201 agacctgaac ttgctgcatt tgtaattcta gtattatcat ttcaaaagtt ctttggcatt
     1261 ccgataacta catattgtat aaataaagaa ttaaaattta aaagagataa tggggatttt
     1321 ttgaaagatg agtcaaaaag taattttaaa cttccaagta tgagaatttt taaagaagat
     1381 ttaggtaaaa aatcatctac tctagtgtat cttgcaaagc tttcatttgt tgctatgatt
     1441 ggtaattttg taggattggc tactttaata ccaggattta cccctgcaaa ctattattta
     1501 aatcctaata tagcatgttt gttagtaggg ttgattttta cacgaatagg ttttttagaa
     1561 aaaggaattt tagataaatc tcaatctaat ggaattataa tgtttggttg tatgttgatg
     1621 ttacctggag gacttgctaa ggtaactcca acagcattgt ttgggatgat tgttccaact
     1681 ataggaatac ttgttgtagg ctctgtattt ataatattat ttactgcaat tgtaggaaaa
     1741 ctattgggat attctccaag aatgtcagct gcaattggtg taacttgtat gctagcttat
     1801 cctgcaacac agataatttc caacgaaggt attgatgcta tggaaggaac tgaagaagaa
     1861 aaacaaaggg caacagatta tttattacca aagatgatta taggaggatt tgttactgta
     1921 accattgcat cagtggcatt tgctagtata ataggtccaa ttatatttgg ttgatagtaa
     1981 atatacttat aggaggaata ggaaatgata ggaagtatat ctataaatga gatagataaa
     2041 aaaagattca aacttgatga tttaagtaag aaaatatggg aaaatccaga aaaagcattt
     2101 aaagaattta aagcatgtga aaatacagca aactttctaa gaagtgaagg atttgatgtg
     2161 gaagtaggtg taggaggttt agccacggct attagagcta gttttggttc aggtaaacca
     2221 gttataggtt ttatgggaga atttgatgca ttacctggtt taaatcaaaa agtttcaaca
     2281 aagcaagaag catttgaatt gggtgcatat ggtcatggat gtgggcataa tttattatgt
     2341 acagcacatg taggggcagt agtagggctt aaaagagaga tgatagaaaa taacttaagt
     2401 gggactatag tattttatgc atgtccagga gaagaacaat taacaggtaa aggatttatg
     2461 gctagaggtg gagcgtttga agggttagac ttagctataa attttcatcc aaataaaatt
     2521 agtgaagcta caattggtac ttcaacagct gttaactctg taaagtttca ctttaaggga
     2581 aagactgcgc atgcaggctc agaccctcaa aatggaagaa gtgcactaga tgctgtggaa
     2641 cttactaatg tgggagctaa ttatttgaga gaacatgtta cttctgatgt aaggattcat
     2701 tatacaatta cagatggagg agtagcaccc aacatagttc ctgataaagc atcagtatgg
     2761 tattatacaa gagctttaag tagagaagct gtagaaagta catatgaaag acttgtaaag
     2821 gtagcaaaag gagctgcaat gatgactgaa actgaagttg aagtagagtt tttaggaggt
     2881 tgttataata ctttaaacaa tcatgtttta gcaaatcttg tatcagaatg tatgaatgag
     2941 attaaacaag agccttggac tcgagaggaa ttggattttg cagctgaatt agatagacaa
     3001 agcccacaac aatataaggc tatgataagt aaatataatt tgccagaagg aactcatcta
     3061 tattatggtg gaggaaatgt tacaaacttt aatagttatg gttctactga tattggagat
     3121 gtaatgcata tagttccgac agcatacttt tttacagggt gtacgaatct aggagcacca
     3181 ggtcatagct ggcagtttac atcttgtgca ggaagttcta taggagaaaa aggtatgata
     3241 tatgcatcaa agattatggc tatgtttgga gctaaaatat taaataatcc agagatagct
     3301 aaaaaggcaa aagaagaatt tgataaatct atgaatggta aaacatataa atgtcctatt
     3361 ccagatgata ttccaactcc ttaaaaagac atgaaattta atatataaaa ttataatttt
     3421 tatatcagca tcatttcatt actaaaagaa agtgagattt tgctgactat ctgattagga
     3481 ggaaggaata tgaaaaattt ttttaaagaa gcgcagtcaa tagaagggga aattattaga
     3541 tggagaagat atttacatga aaatcccgaa attggttgca atataccgaa aacagtaacc
     3601 tttgttacca gtactttaga aaaaatggga tattctccga aattgacgtc taataatgga
     3661 atagtagctg ttttagacag tgaaaatggt ggaaagacct tattattaag ggcagatatg
     3721 gatgcacttc ctattatgga ggagacggat ttacctttta aaagtaagaa taaagatgca
     3781 tctcatagtt gtggacacga taatcacaca gcaatgctct taggagcagc taaaatactt
     3841 atggataata aacacttact taaaggtaga gtaaagtttg tatttgaacc agatgaagag
     3901 actaccagag gtgcaaaatc aatgattgat gatggtatat tagaaaatcc tcaggtcgat
     3961 gcagctatgg catttcacag tatggtgggt aaatttctta acacaggtca agtagcttat
     4021 tcacgtggtc cagctatggc atctgcggat atatttgaaa taacagtaga aggaaaagca
     4081 actcatggag caagccctga atttggagtg gacccaataa acataatgtc gcatatacaa
     4141 atagcattaa acactatact ttcaagagaa aaacctcaga aagaacctgt agttttaact
     4201 attggtatgt ttaatgcagg aaacgtacca aatgctatac caaataaagc atctatgtca
     4261 ggaactttaa gaacatttga ccaatttgtt agagaaaaat taaaaaatag aattattgaa
     4321 atatctacag gaatagctaa atcatttgga ggaaatgcta aagtggaatt taaaatgggg
     4381 actcctgcag ttataaatga tttggatgta ggagatgaga ttgtatctta tgttaaagaa
     4441 gttataggaa atgaaaatgt aatagacttt ccatctgcaa tgggttcaga agatttttca
     4501 gagattttac taagggttcc aggagtattt ttatggattg gaatgggaag tattagtcag
     4561 ggatataatt ttggtatgca taatcctaaa gcaattttta atgaagaggg attagtttat
     4621 ggttcagctt tatttgctca ttgtgcagta aaatggttag aaaataattt tagtagataa
     4681 taacataaaa tattattaaa tatcataaaa ggtatatctt aaaaagatgc aaagatatac
     4741 cttttagaaa gtcatatata attttactta atattagatg ataaaatatt tttatgcatt
     4801 ttggatttta atatttattt tctgatataa ataatttgta taccctttca ggtctacctt
     4861 ttgttgagtt ttgtttttca tataatatag tagcgtttcc agactttaag agactactta
     4921 gaaatctatt agcagttcta actgttacat ttaaacatga agataattcc ttaggagtaa
     4981 cttcatctct ttgtagaatt tcagtaacag atataatctt ttgaatagta agagtagaca
     5041 atttagaact tttggcaatt gtatttatat gtggagttac tttatttgaa acagtaagta
     5101 acttatcaca cagtaaaggt cctattgtct cataattttc gttaactaag aaactgtaat
     5161 ttaaatgatt tattttagat tccttattag cacttatggc actagacata gcttgcttta
     5221 tatcattacc aattccatat ccaacacata ctttaatatt tgtactattt tcaatagatt
     5281 cttttatata acatgcagta aaatttttag ttaatgattt aatcgtttca catttagtga
     5341 aaattttaaa agaattacta tttttttcaa ttataaaatc ggttaattta tcttttttaa
     5401 tactatttaa tattttataa acttggtcta gagtagagtc atcataattt gtatctttaa
     5461 ctgttatata tattacactt ggttgatttc ccttcattct atgtaaatga atatctttta
     5521 ttaagttatt aaaagtattt tttaataaat ctatatcagg gtatatgaaa tgaaagttta
     5581 ctcctgcatt ttgtaacata ggaacaatac tactaaaacg agtagtagaa aaatcgattt
     5641 tcccttcatt ccatagatta atatgttttt ttgcaatctt tttttcaagt tttgctatgt
     5701 cagataaact cattttagat gcattttcat caatactatc cattaagtct ccaaatgtgc
     5761 catttgataa ataatatgat atatcttttg gattatctgc ccagtctgtt aagtcaaagt
     5821 atgctctaga aaaatctaat gtattatatt taaagataag ctttagaaac atatcatagt
     5881 aactttgtaa gtcagtacca aaatcaacta gaggtttttt aatagttcca gccttttttg
     5941 taatagcttt tttggggata gggccactaa ctgcaaatcc atcaaaccta tcttctattt
     6001 ctaaatataa atctccaaca tgagaaaaat ctctatagat atatatttca atttctgcat
     6061 taatatttaa cttttttata gaattatcta caaactcttt taaaaattcg gttgttatga
     6121 ctgctatttt catcatagat tacttccttt tcaattatta taattatttt atattaaata
     6181 ttacattaat ctattgttct agttaaaatc gtatttcaat taaattcaat taaaataagt
     6241 aactctttat aaaaagcaat tatttaggta gaggaatatt tgctaacttt aatagtcttt
     6301 gttaacttta attttattta agttattata gtcttactaa ttttataatt gtagatttaa
     6361 ctactttatg ttcttttatt gtttatgtag ttgatttaga agttatttta accatttatt
     6421 ttaattgaag ttgtttgact tttaaatcct ttgtgtaatc tttatttttc tagccatgat
     6481 aaaagtattt ctatttgtat cactatatat aataatatca tgacaaaata tttattgtct
     6541 aactaattat aatctatcca aaatgaaaat gggtgtgaga tattattaca acaaattgta
     6601 ttagtattaa aaagattaaa aaattaaaaa actaaacagt taaataatta aacgattaag
     6661 tgactgtcat ataacaattt ataattttat ttaacagcca cttaggtatt taagagcgat
     6721 agaattgagt gtatatcaaa ttataataat ttcctagtaa ataattgaaa aataaggtag
     6781 tccattttta tatttgatat ctggtttacc ttgaataagt ggatatagat aatttagggc
     6841 ttcattagtt atataattac attcttcatt tatccactct ttaggaaaat attttgtctt
     6901 gttagctatt ttagaaatat cagtatgact aacttcatac ttataaggat tattgcatgt
     6961 tctattaatg atagccataa ctcctgtata tcctttttta gatagttcta tcccatatgc
     7021 accaacttca gctgcttgtt ttaaatcagt atctgaagct aaatgcatag cacatctttg
     7081 tattacacca agttctaagg tttttactct tgttgtaatt gatgatgata ctataagatt
     7141 ctttagatag ttgcttactc cacctaattg ggaatgacca aatttatcat gagcagaagt
     7201 tttttgttcg catataaagt tgccgtattt atcccttata ccttctgatg caactatata
     7261 agccttattt ttttctttaa atatttttct aacatcttct ataaacttat cttcatagaa
     7321 atcaatttct ggaaggtata ttaaatcaac aagtggagta tcattaagtc tagctagtga
     7381 agaagaagct gctaaccatc cagtatctct acccatagtt tctactataa atataccatt
     7441 atttttgtat atagaaaaat ctaagtatgt ttccattatt agtgtagaaa taagctttgc
     7501 agcagaacca aatccaggac agttgtcagt tcctaataaa tcattatcta ttgttttagg
     7561 aatacctata aattttattt tacaagtagt gtttttgcag tattgagaaa atttcatcac
     7621 tgtgtccata gaatcatttc cgcctatata aaataatgta tctattttat attcatctag
     7681 tatatttata actctatcat aatcataaga atttatttta taatcagcca gtttgtatct
     7741 acaagaacca agaccagacg aaggtgtttg ctttaaattt aatacattat ctttgtttaa
     7801 ggagtctgaa tctattatat ttttgtttaa tataccttca atcccattga taccaaaata
     7861 tactttatca aagtatttag tttctaaatt tttttcataa ataccaactg cagaagcatt
     7921 gataacagct gtagggcctc cagattgggc aataatacag tttctcattt tacatctccc
     7981 tttcattaat attgttatga atggtacttt ttatcgagta aatttatatt tatagtctga
     8041 ttatatatta aatgaactag ctatatcaat tgagttagta tagacaataa atgccataag
     8101 aatgtggtaa aaaactaact ttttatatac aattaatgtc tataatttac cacaatataa
     8161 aggtactttt aaggagtagt caaagtgttg tgaaaaaaaa tatatcagtg taatattaat
     8221 ttacaaagca aaataagttc acagttaaca aaagcagaat aagttcataa aagaaatata
     8281 tacagcaata aaagtaatgc taaataaaag taatactgga ttaaaaaata ttaaaaaaat
     8341 atcaaaaact aatatttaat actaattaaa gtaaaatatg gaggtataca aatatgaaac
     8401 acataaaaat agcagcagga ttagcacatg taaattacgg tcatatatca gccgtagtta
     8461 aagaagcaat ggatgcaggt gtagactacg tacattcaga tgcagcagat atgcatgact
     8521 taaaaaatat gcaacttatg ggtggtcatc aaataataga agcaataaga ccagtgacag
     8581 ataaaccaat agaatgccat atatacacaa gagattgtga tagattattt atagaaaaaa
     8641 tagcagcagc aggatgtaac ttgttgataa taccagcaga acatttttta ggtgctcctc
     8701 ttgcatacat aataaattac tgtagagaat ttggtatgaa agtaggatta acaattggat
     8761 gttacacacc attatgtttt gttgatgaag ctatatatga tatagacaga ttgcaaatag
     8821 tcgttcatgg tgttgatgaa actgatggaa aagataactg gggatggaga aagagtgctg
     8881 ttgatttagt aaaaagagct agaaaaatga tagacgaaaa gaatcctaag tgtgaattag
     8941 caatagatgg tggactaaga tcagacaata tggagccatt aatagaatgt aacccagacg
     9001 ttgtaatatt atcttcagca atattcaagg ataaagatgg tataacagca ggtgttaaga
     9061 attgtagaaa agctatagat gaagcagcta caaagtttgg tttagaataa gataataaac
     9121 tatgataaaa gagatgcttt cttaatgttt taaggaagca ttaattttat gaaaaactac
     9181 aggaagctag actaatttaa ttgatttttt atactgaaac aatataatca taggaggtta
     9241 ttaaatgaaa aaagaaatga taaaagatta tttactgata acaatatcag catttttaat
     9301 ggcattagca gttaattttt tctttgcaga acatacatta gctccaggag gtataacagg
     9361 attatcagtc gtacttagca cattcttatc aatgcctgtt gagaatatat cattaggaat
     9421 atctatgcca cttttagtaa tgggattatt atttttaggg aaaggttttg gaataaaaac
     9481 attatatata actcttatgg gcccattatt cttaaaagta ataccacaaa ctcacataac
     9541 tgacaatcta ttagtagcag gagtgatagg tgggttattg gtaggaactg ctatagggat
     9601 agcaataata aggggctgta gtactggtgg aactgattta gttgcaatgc ttattaataa
     9661 ggtaattaag tttctgaagt tgccagttat attatttatg ctagatggat ttataatagt
     9721 agcgtctgga ataataagta agaattttat gatatctata tattcattga tatctttatt
     9781 actcataata aaaacaatag gatttgtaac tacaaagttt ggtaagaata ctgaagaaat
     9841 taataatata aataatgctt gtgctcaata aaaataataa aatttacttt aaatataaaa
     9901 gtttaaaaaa tataaaagct taaataaaaa gcttaaataa aaagattaaa tataaaaaat
     9961 ggaggtgtct caaaatgaat tttttagttc atgaggcact ttttctatga aatcaaatat
    10021 atttttatca tttagacaat gaaaaatagg tttatctcta tgttgagaca agcctatttt
    10081 tgtttattta taagataata ttttattttt ttcagaatat agttttttta taattgtact
    10141 aagtctagaa atatcattct tacttaaaag agcatttata tttatcagtt cagcattatg
    10201 ttcaaagtct acaggagcag tggttagtat taagtccaca tcatcaaagt tcgtaaatga
    10261 aatagaagat agaggtatag atttaactat attaatctga ttaaattcaa aacttaactt
    10321 ttggactaat aaattagata caccatcact acagtgacat attaaaatag tattaagggg
    10381 agatttgctt ctatctattg ctgatattaa atataaagca agatatccta tttcgtcatc
    10441 attgacttca taattcatca actgtttgat aacagtatta gactctttag cagccttata
    10501 agtataagga aatgttctct ttatatcatt taatatggga tttgtaatag taaacccata
    10561 gtttattctg tgtagaactg atttaaggtg attagcaaga gactttatta atattaaatc
    10621 atcctttaag tttaaattta gtatacttcc ccaatgaaca gctattgctt cagatagatt
    10681 atactctgta atttcttcat ttttaagagg ttcaagagta gtagttttct ttattgattt
    10741 aagtaatcct tctatatgaa gaaggatata acagacctct tcatcaataa attctacgtc
    10801 aaaagcttca cttaggttag cacataaaat ttttgatttt tcaaagtatt catgttctaa
    10861 taattctcta aaaaaacctt cagataagtt aatatatctt ttaattgaaa ttcttttaat
    10921 acatatagct atatgtatta agaagtttat ataatactca tcactaaggt tacttttttc
    10981 tacttgtaag gtgtgtagag tgatttgaga taatttctca aaatcatagt taaatagctc
    11041 cttaaatatt tttgttgaag gggtatcttt gcattcatta gatttcgaga ataatatttc
    11101 tttaagttca gtataacttt tattaaccgt agcaagttca aaaattgctt ttcgaatatc
    11161 tttctctttt ccctctaaat atactccatt attggttttt ctaactagag tcactttaaa
    11221 gttttgcaaa aaattttgaa taactaccaa gtctttatgc acagtagctc tactaacatg
    11281 tagttcattg caaatatcac gaatcctaaa cttagaattt ttaataaata atttacttag
    11341 aatatacctt tttctatctt caggagagta tgcatatata caatttgatt tatcattgat
    11401 atctctaata actttaagtt ttatatcttc attacctaga ataataaccc ctgaaccagt
    11461 ttttttatct agggataaat taaactctaa aagccattca tctaaaataa caaggtcatt
    11521 acgaatagtt ttattactaa cttttaattc ttcggcaata gtatctacag taatatagtc
    11581 tgagctgttg agtaataatt cagcaatatc taataatctt ttgttcaatg caaaccctcc
    11641 taaatcttag ttactattta ttaaccttgg accaatctct ttcaaaagta ttggcaccat
    11701 ctattgtgag ctggttaaaa gaagcttttt taaataaatc atatgttaca gctgcataat
    11761 cacatccaca aagagatgct tgagttaagt ggaatacatt tttaatagaa gctgcaacaa
    11821 ctttagtatt atatccattt attgatataa ggttacatat ttctgtaagg ttttttatac
    11881 catcattccc attttcatca tttctaccta taaaagggaa taggtaatca gcacctgcat
    11941 ttatagctaa tgatgcttga tttacattga aaatcaaggt acatgcagtt tttacacctt
    12001 tactatttaa tatgtttata agttctaagc caacttcaga gaaatttatt tttattatag
    12061 tgtttttatt tatatttaat atcttatcac atgaattaat taattcgtat aaattatttc
    12121 caatgacttc agcagtcaat atttctggat ttaaattagc acatgtctca ataaattcat
    12181 ggaagtttac tttctcttta agatacatag atggattagc tgttattccc ttaaatccat
    12241 actttaaaca cttttgaatt tcatcaagat tagcactgtc tatcaaatat ttcataattt
    12301 aattctcctt taagctattt gtcataaaaa agtgtatata ctctattttt attattaagt
    12361 caattataca ataagattga ttataaaatt ataacatggg tacttaataa aatttacata
    12421 atgatatggt aataataaaa cattgattgt ctaataattg ccataagctt tagtaaataa
    12481 gagttaacag tgaaggtatt gccttaaaaa tcctatgata tgtataattt gaatatgaag
    12541 gcaataattt aagggaggaa aaatatgatt aacttttatc aaccatcaag aataaatttt
    12601 ggacaaggaa ctttaagtga attacctaga ataataggaa aatatggaaa gaaatgtttg
    12661 ttagttacta ctccaaatgt agagccatta gacaagttat ataaaagagt tacattgaat
    12721 ttaatagaaa aaggtataga agttgtacac tttgattaaa aaatagctac gcacactcgt
    12781 gactttagtc atgag
//
LOCUS       DAEORZ010000116        12730 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEORZ010000116 DAEORZ010000000
VERSION     DAEORZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.117"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            117..305
                     /locus_tag="KRH39_002861"
     CDS             117..305
                     /locus_tag="KRH39_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBG8512748.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(465..617)
                     /locus_tag="KRH39_002862"
     CDS             complement(465..617)
                     /locus_tag="KRH39_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBG8512749.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(715..1191)
                     /locus_tag="KRH39_002863"
     CDS             complement(715..1191)
                     /locus_tag="KRH39_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG8512750.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1410..2171
                     /locus_tag="KRH39_002864"
     CDS             1410..2171
                     /locus_tag="KRH39_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8512751.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDSIVIYTRDK"
     gene            complement(2431..4359)
                     /locus_tag="KRH39_002865"
     CDS             complement(2431..4359)
                     /locus_tag="KRH39_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8512752.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSENSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4593..5045
                     /locus_tag="KRH39_002866"
     CDS             4593..5045
                     /locus_tag="KRH39_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8512753.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTNEVKNASDANHLVKYITNKMKEE"
     gene            5048..5356
                     /locus_tag="KRH39_002867"
     CDS             5048..5356
                     /locus_tag="KRH39_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG8512754.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5469..6575
                     /locus_tag="KRH39_002868"
     CDS             5469..6575
                     /locus_tag="KRH39_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG8512755.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6650..9328
                     /gene="mngB"
                     /locus_tag="KRH39_002869"
     CDS             6650..9328
                     /gene="mngB"
                     /locus_tag="KRH39_002869"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG8512756.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPTSLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9645..11978)
                     /locus_tag="KRH39_002870"
     CDS             complement(9645..11978)
                     /locus_tag="KRH39_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8512757.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVGSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDISLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAEMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11953..12669)
                     /locus_tag="KRH39_002871"
     CDS             complement(11953..12669)
                     /locus_tag="KRH39_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8512758.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         4760 a         1780 c         1655 g         4535 t
ORIGIN      
        1 attcttcaat atgtgtaagg caaatatata tttctattgc aaatttttgt tatttatgat
       61 aatataaata tgtttttagt ctttaaatag ttatcatttt aaggaggtgt gcgtagatgg
      121 caaaagtatg tagcgtatgt ggtaaaggta aggtttctgg aaaccaagta tcacactcaa
      181 ataaacataa taaaagaaca tggtcagcta acttaagaag cgtaagagcg attatagatg
      241 gagctcctaa gagagtaaaa gtttgtacta gatgtttacg ttctggtaaa attgaaagag
      301 cttagtaacc acgtttaaaa gcctacttcc gtaagtaggc tttttaattt aatcattttt
      361 actctttctc ataaatttta ggattatact tgaaatccat tttggtaatt ttattgttac
      421 tactttcatc aatactcacc ccacacttta tagttaaaac tattttaaca aaaaaataat
      481 aatgctccac atccaataag tattaatgcg gtaagtgctg accaaatcca gccaggaagc
      541 aacattgaca gaattacagc cattcctgtt gctagacaaa gtattcctag caatacctta
      601 gacttgttat ttctcatgga acacctccac atagaacctg tatctataaa tattttcata
      661 ttcaagttta tgcgacagtt ttccatttta gaccaataaa cacattatgt aattttaatt
      721 tttcatacta gaattattac tgaactccga tatagattct acaatatcca aaattccttt
      781 ttttctactt aacctttcag gtccactata taaaacaact ggtataactt taggaagttt
      841 aaaattttta tcttctattt cttcaaggct ttttttcata ataaaacttt tccaaatttg
      901 ttctctatac gatttcaatc ttgggacaat ttcagtatcc atttctgttt gaaattctaa
      961 cactatataa tgtgatactt tgccactttt agaaaatacc ttataaatta tatcaacttc
     1021 ttttccttta tattcacata taaaagatgt ttctaactct atatttttaa gagttaattc
     1081 ttttgctatt gagacacttg taaaattttg aatcaaactt aaaaaacttt ctttatctga
     1141 atacattctt ctgtactctt ctcgtttttc attgcttctt tttaaattca tacagttcct
     1201 cctagactat agaccctcta ttttttagta aagatagtct ataaatttta ttttttaatt
     1261 ttcattagtg agtattgaca gttgtgtgta aaatatccag taaagatatt tttttatatt
     1321 tttattaaaa gatataaatt tttatatttt aatgtattga catttacgtt gcgtaaaagt
     1381 gtatatttaa tttataggag gtactactta tggaatatac agttcaaaaa ttaagtaaac
     1441 tagctggtat aagtactaga acacttagat attatgatga aataggtctt cttaaaccac
     1501 ttaaaataaa ttcttcaggt tatcgtatat atggtcaaaa tgaagttaat aaacttcaac
     1561 aaatactttt ttatagagaa ttaggaatta acttagaaaa tataaaaaac attataaatt
     1621 caccaaactt cgatagttta agtgcgttaa aagaacacca taataaatta ctagccaaaa
     1681 gaaagcaaat tgatttacta atagaaaatg taactaaaac tatagctctg aaggagggaa
     1741 aatacactat gacagatata gaaaaatttg aaggttttaa agaaaagatg attgatgaaa
     1801 atgaaaaaaa ttatggaact gaaattagag agaaatatgg taaagatatt atagatacat
     1861 caaataaaaa acttaaaaat atgtctaagc aagattatga aaattggcaa aacctaaata
     1921 tcgaaataat tagtaaactt aaaaaagctt ttaaaactgg tgatgcctct agtgaactgg
     1981 ctcaggaagt tgcaaaatta catcataaat ggttgagtta cacttggaac acatactcta
     2041 aagaagctca ttcctctcta gcacagatgt atgtatatga tgaacgtttt acatcatatt
     2101 atgatagaga acaacctgga ttagctaagt ttttaaggga ttctatagta atatatacaa
     2161 gagataaata atataaaata ataaaggtgg tgtctcaaaa tgaacttttt agttcatgag
     2221 acactctttt ttgtatgaaa tcagatatat ttttatcatt ttatcaatga aaaacaggtt
     2281 tctctctcta ttttgagaca gaatctttat tgtataataa ttataatcaa tacaaaataa
     2341 taacaaatta tacaaaactt acttatacta tttatgctaa aaacaaaaaa tattttatta
     2401 aagtttttaa aacttatgta atataaaaat ctatactata gacttgatta tggaaagaaa
     2461 attatcaaaa tcattacaat tagatatact atctctaata tcattgtcat gaattatctt
     2521 taaaagtata tcataaatgg agtttatttt tatataatca tctttattta tagctaacat
     2581 catgattaat ttaacagtag aattttcact ccaatatatt ggttcattca atatggaaat
     2641 acaaatctta gttcttatag agcttaattc cataggatga ggtatagcta aaaaatcatc
     2701 tatattggta gaagataaac tttctctgta aaatacagat tcagcaaatg atggaaatac
     2761 aatttcactt tgagttaatt tgttgcacat caactttata agctcatctt tatctttgat
     2821 tttaggatta acaataaata aatttttatc aaagaaattg tccaaaagat tactataaat
     2881 atgggaatta ttagtgattg acttagatat attctctata tcttttttta atagttttaa
     2941 atccactaat acaataggaa tactatcatg atttaaagga attgtcgata ttattatatc
     3001 tacatctgac aattcactag ctaaaaattg attaaatgat agacattgaa gtatatttat
     3061 tttatcatga aaaactttgt ttaattgaac ctctaataac ctacttgaac cataccctga
     3121 accacatact aatactacgt ttttacactt aatattactt tgaaaaaatc tctctattcc
     3181 agcacctata tgcagggata catatccaat ttcatcttct gttaaagagt aaatactatt
     3241 tttaaaaact ttttctatag catttaaagt tatttcaaat gctaaaggat aattactctt
     3301 tattgtattt ataaggggat tcaccttatt taaattataa gatttagaat tgagaattga
     3361 tttaaagtgc agtactaaat catcaaataa tacactatca tttctaaggt caaaagtata
     3421 ttgattgttg attacttcta aaatttcttc aacatactct ataattttag tatctaccct
     3481 attggaaacg ttatccaaaa gagaactttt agttataaaa tgattatata gataaactct
     3541 gtctgccttt gaaataatta tagtatattt actctcaata taattaaata tattttcaac
     3601 agttgtattc gattgaaaat ctgttaaaac aacatctaaa acattatcta agcagtaacc
     3661 atcaagtatt ctacttacag taatagctaa atggataata aagtttttta gattaaaatc
     3721 taggaaattg atattaaatt tattaatttc acttaacact accctttgaa gctctattaa
     3781 atctacatta ttaaataatt ctatttctct atctgtaatt ccaattatat agttttcaaa
     3841 attcctattt atcatattat tagaaataca tcttcgaata tctatttcct taccttcaac
     3901 tttaactcca taatatggct tagaaactaa tattaaatta tactttgaca aaataggctt
     3961 tattcttttt atgtcattta gtattgtaaa tttactcaca tacaagctat tagctaaatc
     4021 ttctagtttt atatatgtat tgttatagag aatatatttt aaaatatatt gatttctttc
     4081 tataggagaa tctggtattt cacttgaatc cataatatca tcagatatta atgctagata
     4141 ctgttggtat aatgctaaat taagaatatc tatataatat ccttctcctt tttttatttt
     4201 aataatagca ccatttttct ctaaagttcc atttatagaa gtaatatcat ttcttatagt
     4261 tctctcactc acttttaaat gattacttaa agttttacaa gtaattttat catcactttc
     4321 atttaataat ttaaacaacg tgagcaatct cttgtgcata aatatccccc cttatagaat
     4381 ttcttctaaa ttatactatt tacactcata taatacaatt actctaataa aattgtatta
     4441 taataattta tttccacttc tatatggaaa ttagttatta taataatata aatttccatg
     4501 tcgaagtgga aattagttat atttaattta attaggataa tgattacaat ataaatataa
     4561 gttaagacat tatgaatgga gtggaaaaat atatggtact acaagaaaaa tatatattta
     4621 tagataaaga cttaaaaagc aaagaagaag tattaaattt tattgcagat agtgcttata
     4681 tactaggtat tacaactaat aaagaggctc ttttaaaaga ccttctaaaa agagaaaatg
     4741 aatttccaac aggaataaaa gatggatttg cgatacctca tacaaaaagt gtaaatgtaa
     4801 aggaagcttc catactctat attaaaacaa ctaacgagtt agagtggaaa actttagatg
     4861 atagtaaggt tagatatgta tttagtttgt tagccccaca taaaaatgaa ggaaatatac
     4921 atttgaaaac tctatctaaa ttagccacat gtttaatgga ggatgatttt acaaatgaag
     4981 ttaaaaatgc tagtgatgca aatcatttag taaaatatat aactaataaa atgaaggagg
     5041 aataattatg aaaatagtag caataacatc atgtatagca ggattagcac acacacctat
     5101 ggcagcaaaa gctttagaac aagctgcatc aaaaatgggt catgaaatca aagtagaaca
     5161 acaaggtgct atgggacgag taaatactat aacaaaagaa gaagcacaag atgcggactt
     5221 tatacttata gcatctgacc aaactatatc tggaatggaa agatttgatg gtaaaaaggt
     5281 gataaaagtc aaaataggta tagcgttaaa aaaaccagaa gctgtcttaa caaagtgtat
     5341 agaagctatt tcttaataaa aaatctcact ctaatataga atgagaaaaa tcaaaaatta
     5401 aaaaattgtc taatgtaatt ataacatatt atcaaaaaat ataacaaact tatataggag
     5461 gattaaaaat gagtaaaaaa aataaatctc ttttaagtga acttaaggga cacttaatga
     5521 caggtatctc atatatgttg ccacttgtaa taggctcatc tttagttgta gctatcccaa
     5581 agcttacagc tttagcaatg ggtataacaa gtttagatgc ttataaaaat gtatctggtt
     5641 tttatcatat tctttactta atggaacaag ttggttggac tggcataggt ctattaaata
     5701 cggtattagc aggatttata gcattttcaa taggtgagaa accagctatg ggtgcaggtt
     5761 ttattggtgg gctcatagca agtaatacta atgcaggatt cttaggagca gtaatagcag
     5821 gtttctttgc tgggtatgtt tgtaagtttt taaagaaaaa aattaaaata tctgggtcag
     5881 catcaggaat gatgccactt ataataatgc cacttataac agttggatta actggttttt
     5941 taatgagtgt tgtattagca ggacctttag gaaacataaa tacttcatta aatgcttggg
     6001 tagctgaaat gtgtcaaaat ggaacaaatt cggtggtatt ggctttaatt ttaggcgcaa
     6061 tgataggatt cgatttaggt ggaccagtaa ataaagcagc ttggatggca ggaaatgcat
     6121 tattgttaga gggaatctat ctaccagcga tattggtaaa ctgcgcaata gttatacctc
     6181 cactaggata tggtttagct acattcataa gaaaatcaaa attttccaat gcattagctg
     6241 aaacaggaaa aggcaatata gttatgggta taattggaat aacagaaggt gcaattcctt
     6301 tcactttaac aaatccatta aaattagttc ctgttaatat gataggatgc gctttgggtg
     6361 taggattatc agctttgtta ggtgtacatg ctataatgcc tccagttggt ggattatatg
     6421 gatttatatc aataggttct ggatgggctt acttagtagg tgcaatattt ggagctttag
     6481 taatagcaat attatcaaca actttagtag acttcaatga agatgaaact acggaaaata
     6541 cagaatttga tgaaatagag ttagatataa tgtaaattaa aaacttaatt ttagataatt
     6601 gagaattgag tgtactctca attctcaatt aaattttggg aggattttta tgaaaaagac
     6661 aaaagtacat gtagttccac acagtcattg ggatagagaa tggtatttta caacatcaag
     6721 gtcaaaaata tatttaatga aagattttca agatatatta gacatattgg aagaaaaaga
     6781 agactttaaa tactttacta tggatgcaca agcttctctc ttagatgatt atttaaaatg
     6841 gagacctcag gataaatata gaataagaga tttagttcaa agaagaagat tagttattgg
     6901 accttggtat actcaaacag accaattggt tatatcagga gaatctataa ttagaaacct
     6961 ttactatgga atagatagat gcaatgaatt tggtgaacct atgagggttg gatatgtacc
     7021 agattctttt gggcaatcag ctcaaatgcc acaaatatac agaggttttg gaatagatag
     7081 ttcattattt tggcgtggag tttctgatga tatggtagat acaacagagt ttatatggaa
     7141 aggttctgat ggttcaaaag tattagcagt tcaaatacca tttggttatt actatggagg
     7201 taatatacca gaagatgatt cagatttaaa agaatattta aaagatataa tcggaaaact
     7261 taaggttaaa gcttctacta ataacgtata ttttccaaat ggatttgacc aagcaccaat
     7321 aagaaaaaac cttcctgata tattaaaaaa agctaatgaa atagataatg aaaatgaata
     7381 cgaaataagt gatatagaaa actatataga ttcagtaaga aatgaaagaa atgaatttac
     7441 agagcttaaa ggtgaatttt taaatgctaa acatatgaga atacacaaat ccatattctc
     7501 ctcaaggtca gaccttaaaa taatgaataa caaattagaa aattatatta caaatgtact
     7561 tgaaccatta ttaacattaa gttattcact tggaaatgaa taccctcatc tagttataaa
     7621 agatatatgg aaacttatgt ttgaaaatgc agctcatgac agcataggtt cttgtaatag
     7681 tgacacaaca aatgaggatg tatacacaag atacaaacaa gccagagatt tgagtataaa
     7741 tttattagat ttgcatatga gacttataag tactcaaatc aaaaataata atgaagaaat
     7801 aacattaaca gtattcaatg gattcccaga agtcagaaat gaagttgtag aatttgatac
     7861 ttatattcca ggtcaagatt ttgtgataaa gaataaaaat ggagaaattc ttaattatat
     7921 aataaaagaa aaagaagata ttacaaatta tatattaact caaacaataa gattaaaccc
     7981 aagtaaaaaa atatatatac ccaaaactgt atatagagca aaaattgcac tttcagtaaa
     8041 agatatacca ccaatgggat atactcaact agtttttgaa ttggatacta aaaataaatg
     8101 tgaaatagaa acaagtaatt gcaaggaaat agaaaacaaa tactacaaaa taacagccaa
     8161 taaaaatggt tcattagaaa tattagataa agaatctaat aagacttata aaaaccaagc
     8221 tgtaattgaa gacaatggag atgatggaga ttcatacaac tattctcctc caagaaaaga
     8281 tatttatgtt tcatctttag attctgtatc ttcagtagaa gtgctaaaat caaacattca
     8341 agaagaaatg atacttaaat attcattgac cataccaact tcactggaag aaagggctat
     8401 aggtaaagtt tctgtgaaaa tgccagtaac aatgaaaatc accttagaac ctaaagaaca
     8461 aataattaag tttaatgtta atataaaaaa taaccaagca ctatctcata gagtaagagt
     8521 tttatttaat acagaaatag catctaagtt ttctatagca gatcaacaat ttggaataat
     8581 acaaagacca acagtattag aaaaagacct tgcattgtgg aaaagagaca attactcatg
     8641 gcaagaaaaa cctataacta tcgaaccaat gcaaagtttt gttactttag aggatggaca
     8701 aagaggaata gcattaataa ctggttgtgt aagagaatat caaatagttg gtgataattt
     8761 agatacaata gcattaacac tattcaggtc atttggatat atgggaagag aaaatttact
     8821 ttatagacca ggaagagctt ctggagaaaa aataatagca acaccagatg cacaattact
     8881 taaagagtta aactttgact ttggtcttta catatataac aataaatttg atgaagcaaa
     8941 tgtagctaat acagctaaga gattcttaac acctatacaa atatatgaat atgctgattt
     9001 tttaaatggt agacttatat ttgcattcaa tgacgaaaaa caaatatatg aaaatgaatt
     9061 tagtttgatg aatgtaaata attcaaattt cacagtaagt gcaataaaga aagaagaaaa
     9121 aggagatgga attgtagtta gaatcttcaa tggtatgaaa aatgaggatg ctaagggaag
     9181 tcttaacatc aataaaaatg tcaatgatgc ttatagtgca atgctcgacg aaatctatga
     9241 taatactagc aagcttaaag taaatactaa aactgtagag gtaaattctt tatcacattg
     9301 taaagtacaa acaatagtaa ttaaataata taaaatagct tatacaagat ttcaaaataa
     9361 attgctagta ctatgttaat agtaaagcca gatattatgc tatttataag ctctgtaaca
     9421 ctaaataaca ttttgtaaaa taaaaaggtg aaccttgtat attaggattc acctttttta
     9481 aataaatcaa cattctgttt taaaataaca tttataagct gttttgaaat aatgcttaca
     9541 aattatttta aaacaatacc tataaatttt tatttaaaga atacacataa atatttccag
     9601 attcttttat tctatagact ttataattat taaagaagtc taatctaatt tttgttaaaa
     9661 gtaattgtaa ctttgaataa atcaccatcc acacttatat tcatcttacc accctgtaat
     9721 tctataaaac tttttgctat cgctaaacca agaccagaac cttctgtgtt acgtgattta
     9781 tctcctctta caaatctctc cattatatca tctacactaa aatcaatctc agatgcagac
     9841 atatttttaa gcataatatt tactttatta tctgtttcta aaatatcaat atacactctt
     9901 gaatttggca ttgcatattt gcttatattt attataagat tttcaaatac tctaaatgta
     9961 cgctgactat ctaatggtaa tattattttt tcattagaaa agttattctt tactattaga
    10021 gatgaatctg caagtttgtc atctaattct aaaagtgtct gcttcattag agatacaata
    10081 tccacattta ctatattcaa ataaacatct ccactatttg ccttgcttac ttcaaacaaa
    10141 tcttctatta gatgttgcaa tctttgtgac tttctgtcta atgtatcaat atacaactta
    10201 cgattttcat ctgttatact ttcatctttt aataaatcta tataagtgat tatagatgtt
    10261 agaggtgttt ttaagtcatg tgatacattt gatataagct ctgttttcat cttttgactc
    10321 ttaacctctt cgtcaacagc ttttttaaat ccagtttgaa tatttcctaa ttcgtctttt
    10381 ataggattaa aaaatcctaa atctttttcc atatttacat ctaagtttcc atctgaaatt
    10441 ttctttgtaa catctaaaag ttgattatat tcatatattg tctttttaga gtaatctgac
    10501 agtatcttaa ataatactac tgaatatatt aatgcgagta ttattccaaa gaaccacata
    10561 ctacacatta taactataat caataaattt attcccaaaa ttgtaactat agttttattt
    10621 ggcttttctt ttagatttat tgttctaatt tgattaataa ctgtttttcc tgcttttttt
    10681 agccatacaa aaaacattcc tgtcacagta tgttttgtaa aatactctct tattccaaca
    10741 tttaaaatat gttttaaaag tattataaat acaaatatta ttgagaatat tacaaaccaa
    10801 taagttaaat tcattaaact taccaatata ctagccattt cagcacttat gtcattgttt
    10861 ataaggtact ctacataatt cccactcaag gtattactta ttataacagt aggatataaa
    10921 tctacaagcc cctgtataat agcaagagca ataaagagta tctctaacgg tattcttgat
    10981 aatgtatcaa atcctagcaa ctctttagat tttctatatg ggaatgctaa agctacaata
    11041 acaactatag ctaatataat ttttaaaatc attccagaaa tcttataata agaattattt
    11101 tctacattgc ttatatatga aattatacca tcatcaaaat aagtatttcc ataaccatac
    11161 tctacactat cacctttact tattacttta ggtacagcat atactaatgt tatatttttt
    11221 attggagata acttatatga aattgaattt cctcctaaac ttatatccat taaatcttct
    11281 cttgctttag ataacaacat ttgattaata actttttgat ttactccata agaatccaca
    11341 atatttactt ttccatcttt atcaaaattc attacaacgt agaatctata gttttctttt
    11401 atatctgtaa tctgcttttg tctttcctca tcattcattt ctgtattttt tgactgattt
    11461 tcatcagaat tttcttttga agcatcattc tttgttgtat caagtactcc taattcttta
    11521 ttatcattaa aaaattcaaa attgctttct ttatcaacta aatagtactt taaattcttt
    11581 cctgacgctt taatgaaatg ctcccaattt tcaaagtttt cattaaaatc tgcattaata
    11641 ctatcatctg tatagtaagt ttctgaacca tcataagtat ctttttcttt acttacattt
    11701 tttatatagt tcatagtttc ttcattgata aatagctttg atggttgtat catcttacct
    11761 tcttgttctt ccttcatatt atgatataaa acataacttc ctttatatat actacctaca
    11821 aagctttcct gttcaaatgg attctttgct ccacctttag cacctgcttg tatagtagaa
    11881 tattgactaa tcatacctaa tgatgctgat aacataagta taaaaactat aatagtaact
    11941 atatatttat ctttaaattt tttcaatttt gtatccaaca ccccacacca cctttaaata
    12001 tttaggattt tttgggtcaa tctctatctt ttcccttata ttcctcacat gtaccataac
    12061 tgtatctgta tttacagcat tctcattcca aactctttca tatatttcat cagcagaaaa
    12121 aactcttcct gcatttctca taagtaaact tagtatttta aattctatag gagttgtttt
    12181 tatatacttt ccatcaatac taacttcttt tgtattttca tttaattcta atccgcctat
    12241 tgcaaacaca ctatcgtcac tttctacctt ttgctcttta tttccaacca tgtttagata
    12301 cttagtgtac cttctaagtt gtgaattaac tctagccaat agttctaatg gcttaaaagg
    12361 ttttgttaca taatcgtctg ctcctatatt aagccccatt attttgtcaa cttcctctga
    12421 ttttgcagat agcattatta ctggaaaatt atagttttct ctaagcttca ttgtaagtgt
    12481 tataccatcc attttaggca tcattatatc aactatagct aaatgtattt cttggttttc
    12541 tataacctta agaccttcta atccattgta tcccttaaaa acattatatc cttgatttaa
    12601 tagatatatt tctatagcat ctgctatctc cttttcatct tctaccacca aaatattata
    12661 tgaattcatt ctaatctcct ttatattaat ttataaattt tctttacata tatgattata
    12721 caatatgata
//
LOCUS       DAEORZ010000117        12605 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEORZ010000117 DAEORZ010000000
VERSION     DAEORZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12605
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.118"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            94..459
                     /locus_tag="KRH39_002872"
     CDS             94..459
                     /locus_tag="KRH39_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512759.1"
                     /translation="MNYHTKEELMEALRVVSSSIINCEKVQPKFAEGTSQHSLLRNRI
                     KALHISKALIESEDIIDKYTKEELIEALPPITSIISKCEKGQMKFSEETSHHTRFKNI
                     IEAMYTSKSLIMDEISKRD"
     gene            719..1918
                     /locus_tag="KRH39_002873"
     CDS             719..1918
                     /locus_tag="KRH39_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBG8512760.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVISVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVVKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDDVLKRDIYLTKICRYLSCNEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKITEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            1970..2473
                     /locus_tag="KRH39_002874"
     CDS             1970..2473
                     /locus_tag="KRH39_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8512761.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYYVYTVFDKVVAQIMYTYEWSDWKNGNFLWIQSVYV
                     DKEYRRKSIFNYLFNYIKNICGKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            2580..2897
                     /locus_tag="KRH39_002875"
     CDS             2580..2897
                     /locus_tag="KRH39_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512762.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            3055..4002
                     /locus_tag="KRH39_002876"
     CDS             3055..4002
                     /locus_tag="KRH39_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG8512763.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRTVNLIPFYTIYQFIVDSNIDFMKATVNIIENIGIFIPMGIFLSIVCKNLNKKTIII
                     AIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGITIYINMNKLFPNDIKRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDTVDIVGTFESFK
                     KGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYEEFDV
                     KNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            4117..4902
                     /locus_tag="KRH39_002877"
     CDS             4117..4902
                     /locus_tag="KRH39_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512764.1"
                     /translation="MEIIKYILYFFIYSFLGWAVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPHRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            5194..6513
                     /locus_tag="KRH39_002878"
     CDS             5194..6513
                     /locus_tag="KRH39_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG8512765.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(6588..7016)
                     /locus_tag="KRH39_002879"
     CDS             complement(6588..7016)
                     /locus_tag="KRH39_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512766.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RRKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(7107..8483)
                     /gene="hydG"
                     /locus_tag="KRH39_002880"
     CDS             complement(7107..8483)
                     /gene="hydG"
                     /locus_tag="KRH39_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBG8512767.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIDKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(8484..9569)
                     /gene="hydE"
                     /locus_tag="KRH39_002881"
     CDS             complement(8484..9569)
                     /gene="hydE"
                     /locus_tag="KRH39_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBG8512768.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLTLDEILE
                     CCDIGYKIGYRTFVLQGGEDTYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(9605..10813)
                     /gene="hydF"
                     /locus_tag="KRH39_002882"
     CDS             complement(9605..10813)
                     /gene="hydF"
                     /locus_tag="KRH39_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBG8512769.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIRILPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(10828..11079)
                     /locus_tag="KRH39_002883"
     CDS             complement(10828..11079)
                     /locus_tag="KRH39_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBG8512770.1"
                     /translation="MKKVAVISAILEEPKECQLKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEI"
     gene            complement(11460..12500)
                     /locus_tag="KRH39_002884"
     CDS             complement(11460..12500)
                     /locus_tag="KRH39_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBG8512771.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLESKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         4657 a         1563 c         1904 g         4481 t
ORIGIN      
        1 gatgtgtata aataataatg atgtcagttg ataatgatat taaattgagg tatactgttt
       61 ctctaaatta aaaaataaag gagtaaatgt attatgaatt atcatacaaa agaagaattg
      121 atggaagcac tgagagtagt atcttcaagt attattaatt gtgaaaaagt acaaccaaaa
      181 tttgcagagg ggacttctca gcattccctt cttagaaata ggataaaagc actgcatatt
      241 tctaaggcat tgatagaatc tgaagatatt atagataaat atacgaaaga agaattaata
      301 gaagctttac cccctataac ttcgattatt agcaaatgtg aaaaaggaca gatgaaattt
      361 tctgaggaga catctcatca tacacgtttt aagaatatta tagaagcaat gtatacttca
      421 aaatcattaa taatggatga aattagtaaa agagattaaa gcttattttg caatttaaca
      481 tgttttaata tacattttat tatataagaa aataagtaag catacatgtt ataaaatatt
      541 ggaataatat acaattataa atttgttaac attaatatct atatgttaaa atatagtaga
      601 agatgttagt aaataatagg atgagttcta ttttaataca attaaattaa gcttagcttg
      661 aaatttgtat ttaaaaagta aagtagtcta gaattaagaa aatggagata gaattataat
      721 ggaaaagtta tactatacaa accagtatat aagagatttt acagcagaaa taattgaaat
      781 aaaagaagta gaaaataaat atcatgtttt attagataag acagcttttt ttcctggtgg
      841 aggaggtcag tttggagacc tgggaaaaat agagaatgat gatgtaataa gtgtgtatga
      901 agatggaaaa aatatatacc atgtattaga taaaaaacct ataaagatac ataaggtaaa
      961 gtgtagtatt gattgggata gaagagaaga tggaatgcat caacattttg gacaacatgt
     1021 attatctgga tgttttttta aaacttttaa tgcaaatacg acaggttttc atctgggaaa
     1081 tgagtatagt acagtagata tagaaggtaa tttagaagag gataaaatta gagagataga
     1141 gttgtttgcc aatgaaatta ttcgtcaaaa tatacaagta gagacattac ttccaacaaa
     1201 aaaagaattg aaaaagattt ggttaagacg tgatttgcca aatactgatg aagaaataag
     1261 ggtagtaaaa ataggcgatt tagatacaaa tgcatgttgt ggagtacatc caaaatcgac
     1321 tggtgaattg agaatgataa aaataaagaa atgggaaaaa cataagtcta gtacaagaat
     1381 agagtttttg gcaggtaaaa gggcggtaga tgatgtatta aaaagagata tatatttaac
     1441 taaaatatgc agatacttaa gttgtaatga agaagatgct ataaacggta ttgtaaatct
     1501 aaatagtaaa gtggaagagg ctttaattaa aaaaagaaga cttgaagaag tagtatctac
     1561 atatcaggtc aaagaaatga ttgaaaaagc aaataaggtt ggcaatattt cggtcattaa
     1621 aaaaatttat gatgatgaag atttgaaata tgtaaataag atagcaaata aaattacaga
     1681 atatgaaaat agtattgcat tattagctgt taaatttaat gataaaataa atttagtttt
     1741 tgcatgttct aaaaatttaa aagatattaa tatgggagtt ttattaaagg atgcaataaa
     1801 tcttatagat ggcaaaggtg gtggaagtaa agtattagct caaggaggag gtaagaataa
     1861 tggaaattta gaagctacac ttgattatgc atttatgaag atagaaaaaa ctctataaat
     1921 atattatata aaaaaactta atagataaaa tagatatgga ggaataagta tggaacaaca
     1981 aaaggactac atagagttat atgaaaatga agaattaata gagataagag aagcccgtat
     2041 ggatgatttg gatacaatag caaaatttaa ttataatttg gctaaagaaa ctgaaggaaa
     2101 agaacttgat atggatgttt taactaaagg tgtaaaagca ttgttattag atgagagaaa
     2161 aggaaaatat tatgtatata cagtatttga taaagttgtt gcacagatta tgtatacata
     2221 tgagtggagt gattggaaaa atggaaattt cctttggatt cagagtgtat atgtagataa
     2281 ggaatataga agaaaaagta tctttaatta tttatttaat tatataaaaa atatatgtgg
     2341 taaagatgaa aatatagttg gaatgagatt atatgtagaa aaagaaaaca taaatgcaaa
     2401 agcaacatat gagtctttaa atatgtatga gtgtgattat aatatgtatg aatacgaagt
     2461 aatacattcg taatataaat ttgtaatacc gttgtactaa tgttataaag tattgattat
     2521 attgacgatt atatgaaata aactggatat tataattaga atttttaggg ggtataaaaa
     2581 tgggcgtttt aaaaggaatg ttttatgtat ttttattttt agatttagtg tcaatattct
     2641 gtttcttttt caataaagga aaaatagcat ctaataaaat tgtgtttaat gcaataggag
     2701 tacttacatt tgtactttgt tttatgcttt ttagttatta tcctaataat aatcttattg
     2761 gaaaatttat tgcatctcta ttttttatat ttggagttgc aggagttatg ttgaaagaaa
     2821 aaaatttttt atatgctaga cttcttttaa ctgtagttat agttttttct acactaagat
     2881 tgttcgttat tcaataaaat gtattcgtat gaaaatttat taaagtctac acatttaagt
     2941 gtaggctttt ttgctttttt atttaaaaat taaggaaatt ttaaggaaat tttaaaatcg
     3001 tcttaaatat ttattgatat acttaaaata ttaatagcga aaggagactt atcagtgata
     3061 gaaagtaaaa agtcagaaaa attgttactg tcaattatat acataatatt tttggtatat
     3121 attttaatga ttataagaat aattttattt agagatgtac caatttacgc tattttcaag
     3181 ggaacgttta ggactgtaaa tttaattcca ttttatacaa tatatcaatt tatagtagat
     3241 agtaatattg actttatgaa agctactgta aatattatag aaaatatagg tatatttatt
     3301 cctatgggaa tttttttatc tatagtatgt aaaaatctaa ataaaaaaac tataattata
     3361 gcaattatac ttgttagcct agcatttgaa ttaacacaat atatttttgc tcttggaagt
     3421 agtgatatag acgatgttat tttaaattca ttaggtggca taattggaat tactatttac
     3481 ataaacatga ataaattatt tcctaatgat attaaaagat ttaaggctat aataggtact
     3541 agtttagttt taggagttat aggattagga gttatttcta aaaattatca taacttacta
     3601 acttttaaat ttagacctga tagaaaaata tcaaaaatat taattgaaga aaatagggaa
     3661 ataattaaag atacagtaga tatagttggg acatttgaga gctttaaaaa aggaataata
     3721 actattaaag caggctctaa taacaaagta aagagaccag agaatttaat tgattctgat
     3781 ggaaatataa gaatttattt aaatgaaaat acaaaattag tatcttatat aattaccaaa
     3841 gaatcaaaaa tggatatagt taaatatgaa gaatttgatg tgaagaattt agatttatta
     3901 aggagatatg atactattaa tgtttggatt gataaagaaa atcagccaaa ggataaacat
     3961 ggtattatgg ctagtaaatt attaattgga ttgtatgaat aaaatattaa acaagaataa
     4021 aagaataaca aatataaaaa acgaaaaaac tatttctata attattttta cgttgtgcat
     4081 aaaataagaa cactagtgaa ggagtagaga taaattatgg agataataaa atatatcttg
     4141 tactttttta tatattcatt tttaggatgg gcagtagaaa gtataggatg ttctatagca
     4201 agcaaaagaa taatcaacag aggtttttta aatggtccaa tatgccctgt ttatggtttt
     4261 ggagcggtaa ttgtgatatc actattagga agatttaata atgtagtaat agtatttcta
     4321 ttaggaatga ttttaactac gatactagaa tattttacag gatttatttt agaaacctta
     4381 tttcatgcta aatggtggga ttattctgac agaaaattca atataaaggg tagagtatgt
     4441 ttaaaaaatg caatatactt tggtgtaatg tctgttttaa taataagatt tatacatcca
     4501 tttataaaat attttgtaag tataattcca catagaatat taatttcaat ggctataatt
     4561 acaacacttt ggactatact tgatttgatt gttactatta taacacttaa aaaattagat
     4621 ataaagttaa acttactaga cgatataatt acagacctta atgatataaa tgtgaaattg
     4681 gataagtttg atagaggaga aatacaagct ttatttaaaa caataaataa cgacgaatta
     4741 gaagttagag aaaaaataaa taaaataaat agtaagttgg accgtataga gtctaatatt
     4801 attttacaaa aaagagttat aaaagctttt ccacatataa aacataaaaa acaacaagaa
     4861 caactagaac attttaaaaa aattattaat gagaaaaggt aattatacaa tttaatgaaa
     4921 acgaatagaa taatggtatt tataagatga ttttcagagg actctataaa aatagaatag
     4981 agtcctttat tttttgaaaa ataatgttta tttgataaaa ataaaatata ttgtttctga
     5041 ataacaaata ttttgaaaat tataaaaaca tatattgaca atttgtgtca aaaatactaa
     5101 cattaagtat ataaagaatt ttataggaga atatctttat aaatcgcaat aattttaaaa
     5161 cataaattta tttaaagagg ggatgatttg ccaatgagcg agataactag tcaaatgata
     5221 agtacagaag aaaaaaaata tgttgctaaa actcaaaaga taccttatta tccagtagca
     5281 tttaaatcag gtgatggtgc gatgctatat gattatgaag gtaatgaata tgtagatttt
     5341 ttagcaagtg caggctctgc aaatgtaggt catggtaata aggaaatctc acaagcagtt
     5401 aaagaacaaa tggatgatat aacacaatat acacttgcat atttccatag tgatcctcct
     5461 gtaaaattag ctgagaagct tgtggaaata gcccctggag ataatgataa aaaagtacta
     5521 tacagtgcaa ctggttctgc ttgtatagat gctgctataa aattagcaag agggtatact
     5581 ggaagaacaa agataatatc tatgtgtgaa tcttatcatg gaagtactta tggagctata
     5641 tctatatctg ctttaagtac aaatatgaga agaaaaatgg gtccattgtt accagaagtt
     5701 tatcacttcc attatcctga taaaaataga acagctaaag aatgtttaga tgagatagaa
     5761 tatgcttttg cacactatct accagcagaa gaggtagctg caatatttat agagccaatt
     5821 gcaggagatg caggaataat agtgcctcca gtagagtggg tacaaggttt aagtaaaata
     5881 tgtaaagaaa atggaatatt acttgtaagt gacgaaatac aacaaggtat gggaagaaca
     5941 ggtaagtggt ttggtataga aaattttggt gttgaagcag accttatagt tcttggaaaa
     6001 tctgttggag gtggattacc tctaggagca gttgttggaa gaacagaaat aatgcaaagt
     6061 cttgatgcac cagcacattt atttacttta gctggaaata caacagtgtg tgtggctgct
     6121 ttaaaatcta tagagataat tgaaaaagaa aacttacttc aaaagagtat agaaatggga
     6181 gattatatta aagcaggatt tgaaaaacta aaagaaaaat acgatataat tggagaaata
     6241 agaggaatag gattatctat aggagttgat atagttaaag gaaaaggttc taatgaaaaa
     6301 catccagatg ctacagctaa gatatgttat agatgtatac aaactggatt aataatgata
     6361 ttcttaggac aatctacttt aagagtgcaa ccacctctag taataactaa ggagcaagta
     6421 gataaagcaa tgaacatcat agattctgca atagatgatt atttaaatgg tagaattggt
     6481 gatgaagtat atgaagtaac tcaaggttgg taaaataaat attaaatgaa aataggagtt
     6541 atctcaattt tagaaagtaa tttctgaaat gagataactc ttttaagtta cccacaaata
     6601 agaaaattgt ttgattcatt aaagactata tttcggatgt cgtttttgtg agtatcagag
     6661 acagtattac gagaataatt acctttattc gtactatctg ttaatttagt tataattaag
     6721 atagctaaaa gaataagaat tactgtgtat aaagcaataa catatggttt tcttttatat
     6781 ttgtatttgt tattgaaaaa agaatggcca taattatttt tatttacttt agatgtattt
     6841 gtattctttt tcttgttacc tttcttttta tcatcctttc ttctattttt agggtaagga
     6901 atgactttgt gtgtattatc aagtttgata tttttatttt tttgaatatt gttttccagg
     6961 ctagcctttt tttctccaaa ttgaatagtt ttattggtat tgttgttatc tttcaatgtt
     7021 aatctccctc ccttaaactt tccctttaga taatattact actaacctat ggaatataca
     7081 ataaataata tataagtaat aaaattttat aggtataaat ctctttctcc acgctctaag
     7141 gcagtcatat tgttagatac tatgtttctt atatcttctc tttctatctt atctatttct
     7201 tctgcgataa gttttttacc cataatttta agttcttcat ctccataatc gattagaaat
     7261 tcatttaatg tagtaagagc atttggtgta catacattat ggatttgtcg acttttagct
     7321 aagctcataa atctatcgcc agttctacct ttgcgataac aagctgtaca ataactaggt
     7381 atatatccat cagtaatcag ttctttaagc acctcaacag ggcttctatg gtctccaact
     7441 tcgaattgtt ctgtattgtc accatcttca tatgctttat atcctccaac acctgttaga
     7501 gaacctgcac taatttgcga tacaccatat tttaataact catttctcat ttcggcagtt
     7561 tctctagtgg acattatgat tccagtaaaa ggaacagcca atctagttat agctactatt
     7621 tttttgaaca tatcatcaga aactaagtga ggaaaatctt ctaagctcat accttcagct
     7681 tttttcaatc tagggaaaga tatagtatgg aaacctacac caaatttttc ctctaagtgt
     7741 tcgttgtgca tcataagtcc tagaacttca aatttagggt cagataaacc aaataaaact
     7801 cctgcaccaa catcatcaac gccagcttcc atagctctat caaaagctgt taaatggtaa
     7861 taatagtcat tttttataga ttgaccatgc atttttataa atgttggttt gtgataagtt
     7921 tcttggaata atatataagt tcctattcct ttttctttta gtaatttata ctcatctaca
     7981 cttgtagctg ctatgttaac gtttaccctt cttatatttc cgttttcatt atatgttgaa
     8041 taaattgcat ctaagcaatc taagatgtaa tttatatcac aatttttagg gtcttcacca
     8101 gcttctagag caagtctttt atgacccatt ttttctaata ttttaacctc ttcctttatc
     8161 tcttccatgg taagtttttt tcttttaaat ttgttgcatt tactaaaacc acaataaaca
     8221 cattcattta cacagtagtt tgatacataa agaggggcaa ataaaaccac tctattacca
     8281 tatatttcat tttttatttg cccagctata ctaaataatc tatctagttg ttttttatct
     8341 tctacttcaa gaagtgtagc tatatcttta taactaagtt tttcccttct atcagcctta
     8401 tcaagtacct tttcaatatc atcagaagtt gaattttttg catcttctag taaagaatta
     8461 ataagttcat ggtttatgaa catttacaaa cgcctccatt caatattatc accgcgagaa
     8521 tagtcaactt caagtcctaa tgacttgatg ttttcttcta aactttggtg atattcacaa
     8581 gcttcttgac cagtaaaggc tttgttatcg taaagagaat attgttttct aaattcttga
     8641 ggagaaaggt ttggcataat tacattgcaa ccagctaaaa gacctgcatt tctaccacta
     8701 gggtttatag atgccaaggc tgttgtagcg ggtaataaaa cttttggaag tagaagtctt
     8761 gtaattgaaa gcataagtaa tgtcttttct aaatctccat gtttataatc cttaaaaatt
     8821 gtatcatggt gtggaataaa aggtcctatt ccaaccatat gaggatttaa ctcttttagg
     8881 tataataaat cacgaacaag gtcttcgttg ttctgaaatg gtgattcaac cataaatcca
     8941 gcacctacct gataaccaat ttctttaagg ttttttaggc attcttttct agttctaagc
     9001 tcaatgtttg gcggatgtaa ttttttataa tgactgtctg tagcagtttc atgtcttaaa
     9061 agatatctat ctgcaccaca taaaaagtat tttttatacg aatcataact cttttctcct
     9121 agtgatagag ttatggcaca atcctcatat ttatttttta tacttgaaac aatttcacat
     9181 attttatcat cagtaaaata tgtatcttca ccaccttgca aaacgaatgt tctgtatcct
     9241 attttataac ctatatcaca acattcaagt atttcatcaa gtgtaagtct gtatcttatg
     9301 atatttttat tagaagattg tattccacaa tagtagcaat tatttttaca atagtttgta
     9361 agctctataa gtcctctcaa gtagactcta tttccgtaat gtttatttct catgtcgcta
     9421 gcttgtttat aaagatagtt tcttaaattt tgttcgaagt tatattcctc attgtcgata
     9481 ttttctatta aatataatag ttcatcaaaa cttaatgaat taatgctgta aagtttatct
     9541 ataaagtatt taatcttatt tttagtcata tttaaatatg taattattaa gtatagttaa
     9601 atttttaata attgaaaagt tcaagtgatc tttctaaaat accttttaca tatgctatta
     9661 agataccata gtttacaatc ggtacattga aagattttgc cttttctatt ctagataaca
     9721 ttccagctct attcatcata caagctccac agtgaactac aagggcgtat ttatttatat
     9781 cttcagtgaa ggatactcca gaactaaatt caaaagtaat atttttgcca gttttctttc
     9841 taatcatatt aggaattttt actgttccaa tatcatcagt ttgtctatga tgggtacatc
     9901 cttcagccat caagatttta tcaccatctt ttaaattttc taaagcataa gcgccattaa
     9961 tcaattcttt aaggtctccc ttttgtctag caaataatat agaaaaagat gtaaggggaa
    10021 tatcttttgg tgtatcttta tctacttgtg gaaatacttg agagtcagta ataacaagtt
    10081 ttggtttttt gcctaaattg gatagagttt cttttaaact atcttctttt gtaactatag
    10141 aaattgcacc attatccaaa atatctctta taacttgctg ttgaggaagt ataagtctac
    10201 cttttggggc tgctttatct attggaacaa ctaaaacaac taaatcattt ggttcaatta
    10261 aatctgatac caatttgaac tcagtgctat ctttaggcaa aattcttatt atttcatttt
    10321 ttaaattttc aattccaatt ttatctgttg atgagacaga aacaactgga catttaactt
    10381 tatttttagt taagtttaaa atttctgatt gattcttaat agtatcaatt ttgttaagaa
    10441 ttaaaatatg agggatattc ttatcattaa acttttcaat tagtgacaaa tcttcttgtg
    10501 atataccaat ctggcaatcc actactagta aagctatgtc tgtcttctct aaaacatcta
    10561 gtgatttacc tattctaagt tcaccaagtt caccaacatc atcaagacca gcagtatcta
    10621 taagaacaca aggtcctata ggaaggattt ccattggtct aaatactggg tctgtagtag
    10681 ttccagcaat atctgagact attgcagcac tttgattcgt tatagcattt attatgctag
    10741 atttacctgc atttctttta ccaaagagac ctatatgtac tcttactgat tgtggagtag
    10801 aatttagact catttgtact caccacctta aatttctttt tttgatatac tagtttttac
    10861 agatacatga ggtatgttgc ccaatttacc agtcaaacta ttaatagtat ccatttcgcc
    10921 aacaactgct atacatacga ttgatacgcc ttcttcttcg caaggaatgc ccattcttcc
    10981 tttaataata cctttaaaac ctgatacaac ctcattgaat ttaagttgac attcttttgg
    11041 ctcttccaaa atagcactta taactgcaac ctttttcata aatatcctcc taactcagaa
    11101 aatatgtctt aaacaaatcc aaaatcaaaa aacttttctt tgtaaagaaa agtttctaca
    11161 aaagaaaagt tattaattac acaagtataa tgtgttatat taaactattt ccttacagat
    11221 tagataactc ttttctggta gggttagctt gtaaaaattt aatttaacaa caattaactg
    11281 aagaaaattt ttatacaatt tatataaatt ataataaaaa ttaactaaag aaaattgaag
    11341 ttacttatat aatagtagta aattgaaatt aagtctatag aaaaaccaat ttttgtataa
    11401 aaataaaaat taaagctagt tatttgttaa gaattgactt tggtaaaata tataattaat
    11461 tattctttaa gcatataatt tataagtaat tttagtgtat tttcaatagc ttctatatgt
    11521 gttctttcat aatggtgact attgtttgtt ccaggaccaa cacatgctat ttgaacatct
    11581 tctccccaac gaatagcttc tgaagcatct gaaccataga aagtaaatac atcgacagcg
    11641 tagtctaaat tattgtcttc tgcaacatcg acaagcttgt ttcttaaatt aaaatcatat
    11701 aatgataaat tatcttttgc aactatagtt acaccatgtt cactagatgt ttgacttggt
    11761 ccagtaggcg ctatatctat tgctacaaat tcttttgttt tttcaggtaa acatttagaa
    11821 acaccatgtc caatttcttc ataattactt atataaaagt tagtagtatg cttaggtgtt
    11881 aaattgtttt ctttaaaata tctacatatt tcaagaacca ttgctacagc tgatttatta
    11941 tctatatatc tagattttat aaaaccagat tctgtagcct caaatctatg gtctaggcat
    12001 acaaaatccc caacacttat tcctaaatct aatacatctt gctttgtaaa tactttttca
    12061 tcaagtctaa taatcattgt ttcttcattt ctttcagtgg cagcttttgt ttgaccatat
    12121 atatgagtag aagcatactt aaatacaaca cttcctctga tttttttacc ttttctagtt
    12181 ataacagtac agttttctcc ctctatagct gcccaacatc caccaccaac tttatggtat
    12241 ttaagagtac catcagatga tatttctttt actatagctc caagtgtatc catatgtgca
    12301 gatattgtta tttgatttga atcatcttca ccaggaagag tagctataag tgcacctttt
    12361 tttgtaagaa tagtgtcaag acctaaattt tcaaaatctt ttttgctttc taaaactgca
    12421 ttttcagtat atccagctgg gcttggaatg tccaaatatt ttttcattaa atttatagta
    12481 ttagttaaat ttaaattcat attgtttcct cctaaataaa tatatttata caaattataa
    12541 cataaataca aatgttctga aaactaaaat taaaagaata gaatctaaac atatttaaaa
    12601 aaaaa
//
LOCUS       DAEORZ010000118        12400 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEORZ010000118 DAEORZ010000000
VERSION     DAEORZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.119"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            170..1432
                     /locus_tag="KRH39_002885"
     CDS             170..1432
                     /locus_tag="KRH39_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG8512772.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            1452..2195
                     /locus_tag="KRH39_002886"
     CDS             1452..2195
                     /locus_tag="KRH39_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBG8512773.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            2368..3753
                     /locus_tag="KRH39_002887"
     CDS             2368..3753
                     /locus_tag="KRH39_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8512774.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            3972..4811
                     /locus_tag="KRH39_002888"
     CDS             3972..4811
                     /locus_tag="KRH39_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512775.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            4801..5244
                     /locus_tag="KRH39_002889"
     CDS             4801..5244
                     /locus_tag="KRH39_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512776.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            5255..5746
                     /locus_tag="KRH39_002890"
     CDS             5255..5746
                     /locus_tag="KRH39_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512777.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(5948..6676)
                     /locus_tag="KRH39_002891"
     CDS             complement(5948..6676)
                     /locus_tag="KRH39_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG8512778.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            6801..7889
                     /locus_tag="KRH39_002892"
     CDS             6801..7889
                     /locus_tag="KRH39_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512779.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            complement(8326..9174)
                     /locus_tag="KRH39_002893"
     CDS             complement(8326..9174)
                     /locus_tag="KRH39_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512780.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
     gene            9373..9738
                     /locus_tag="KRH39_002894"
     CDS             9373..9738
                     /locus_tag="KRH39_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8512781.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            9761..12148
                     /gene="cadA"
                     /locus_tag="KRH39_002895"
     CDS             9761..12148
                     /gene="cadA"
                     /locus_tag="KRH39_002895"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBG8512782.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
BASE COUNT         4712 a         1260 c         2203 g         4225 t
ORIGIN      
        1 cttataaact ataaaaatta ttaagttttt gtatatattt ttatacttta atttgcatac
       61 atctttatac attgaatgag aatatgctaa gtgaaaaagt ttactatggc ttaaatagat
      121 gagattagac ttgaaatttt aaatgtaaaa ataaatctta ggaggaaata tgaaaaaaat
      181 tctcttagat acattaaata gtaaaaaaca agaatacata gattatttaa aagaacttgt
      241 aagtataaag actgaagatg ttggtcatgg aatactaggt ggatttgaaa aagaaggaca
      301 ggaatatatt gaaaaacttg caaattacat aggttttagt gtggatagac aagaaatgag
      361 cgaagaatta attaaaaaag caaaaaatat atataaagaa ggaaatctag gtcacaatta
      421 tcaagataga tataatttaa tttgtaagta tagtgatgat ttacctggta agacaattgt
      481 atttaatggt catgtagaca ctatgccacc gggtgatata agtaagtgga aatataatcc
      541 atatcgtgct acagaagata atggaaaact ttatggtctt ggaactgctg atatgaaatc
      601 tggacttata gcatcaatat tagctgtaaa gcttataaaa gatagtggtc taaatgtacc
      661 tggtaatgta aaaataatgt cagtagtaga tgaagaaggt ggcggaaatg gtaccataaa
      721 tgcagttatg aatggtattg atggagattg ttgcataatt tgtgaaccat cagaacaaaa
      781 cttaatagtt gcacatatgg gatttgtatt ctttgaagtt gaggttaaag gtgtgtctct
      841 tcactgtggt agtaagtggg agggtgtaaa tgcaattgag aaggctatgt tgttgcttca
      901 agatataaaa gagttggaac ataactggct tatgatatat aaacatccac tattaccttc
      961 acctacaata aatttaggtg taataaatgg tggaacagca ggttctactg tgccagataa
     1021 gtgtgtattt aatttatgtg tacacttctt gccaaatata atgagttatg aacaagttgt
     1081 aaatgatgta acaaatgtca taatgacaag ggctaatgga gatttatggc ttaaagataa
     1141 taaaccaaat ataaacatat atcaatcagg gttaggtttt gagatggaca aggattctga
     1201 ttttgtagtg aatgcacata aaatactaga agaaactcta ggaaaaaaat tagagataaa
     1261 aggttcaaca gctggcaatg atgcgagagt aatgaaaaat ttagctgaaa taccaacact
     1321 tatattaggg ccaggttcaa tagaacaatg tcattcaatt gatgaatatg ttgaaattaa
     1381 ggagtattta gatagtatac ttatgtatgc aagtctaata ttaaacttat aaaagtagga
     1441 ggaatatata aatgaaaaaa gtattaatag taggggaatc ttgggtaaaa aatataactc
     1501 atataaaagg gtttgatacg tttgttacaa ctcattatga agaagctgta aagtggttga
     1561 aagaagcaat agaatctgga ggatatgaga cagttcatat gcctgcacat gtagcagcag
     1621 atagttttcc gtataaatta gaagagttaa atgagtatga ctgtataata ttatctgata
     1681 taggttctaa tacgttctta ttatctaata gtacttttat agattgcaat agtaatccag
     1741 atagacttga actaatcaaa gagtatgtaa ataatggtgg ggcgttaatc atggtaggtg
     1801 gatatatgtc atttacagga atagatgcaa aagctagatt tggagaaact gctatcaaag
     1861 atgtattacc aataacaatg atagataaag atgatagagt tgaaaaacca gcaggaataa
     1921 ttccagaagt tattgactct gaacatccag tattaaaagg tataccaaca gaatggccaa
     1981 agtttttagg atataacaaa acagtagcta gagataattg tcctgtttta gcaactattg
     2041 gaggagaccc atttgtagct gttggcgagt ttggaaaagg aaaatctgct atctttagtt
     2101 ctgactgtgc acctcactgg ggtccaaaag aatttactga ttggaaatac tataataaat
     2161 tatgggttaa tatgcttgat tggttaacat gctaaaaatt ttataaaact tgaaaacctc
     2221 ttacttgata aaatgaaact tacaagtcaa gaggttttat tgtataaaat ggttgtatgg
     2281 tagaaaatat ttttaaatca gttaaaatta gtcattttgg ctgataatcg ataagttatt
     2341 agaaacaggg aggtttatat aattaatatg gataatttaa ataaaaagtt tttgatggaa
     2401 tatatagatg agatacaggg agaattaaga gatatatcag ataagatatg gaaaaatcca
     2461 gaacttcagt ataaagaata ctatgcttca aatttacaaa aagaatgttt aaaaaaacat
     2521 ggttttagta tagagggatt agaaggtatg gatacagcat ttgtggctag ttatggagaa
     2581 ggtagaccag ttatagcagt tttgggagaa tatgatgctt tagatggttt gtctcaaaaa
     2641 gtatccacta ctaaagaacc tattgtagat ggtggtgcag gtcatggatg tggacataac
     2701 ttacttggta caggttcaat aggagctgta attgcagtaa aggaattaat caaactaaaa
     2761 aagctgaaag ggactataaa atactatgga tgtccagcag aagaagattt atctggaaaa
     2821 gtattgatga taaaaaaagg tttttttgat ggaattgatt gtgcttttag ttggcatcca
     2881 tttgatataa atactccaat aagaatacca acacttgcta attactctgt gaaatttaga
     2941 tataatggga ttagtgctca tgcagcacaa gctccttata acggaagaag tgcacttgat
     3001 gcagtagaac ttatgaatat aggttgtaat tatttgagag aacatatatt tgataattgt
     3061 agagtacact atgtaactac taatggtgga aaaatgccaa atatagttcc tgattttgca
     3121 gaagtgtggt attacataag aggagttaaa atggagcatg taagagatgt atttggtagg
     3181 attgttgata tagcaaaagg agcagcatta atgactggta ctattatgga atatgacatt
     3241 ataagtggag tatatgacta tattccaaat acaatattaa cagatattct atcacaaaat
     3301 atgaaattag taggtgtgca aaactataat gaagatgact actattttgc agataaatta
     3361 gctgagacag tgtctataga taaaagaagt agtgtttcta gtgtattaag tggaaataag
     3421 gatattacta agatgaatct gcatgatgaa gttacggatg atacatttac tcataataac
     3481 tgtatatcat tatcattaga tattggagat gttagctaca taattcctac agcacagtgt
     3541 tcttgttcag tttggcctat tggtatttct gcccatacat ggcaatcttg tgccagtgca
     3601 ggttctgata tgggatttaa ggcaatgtta ttggcatcta aatctatatc atgttcaata
     3661 aatgatgtaa tgcttgatga attggttata aacaaagcaa aaaaagaatt gaaggatact
     3721 gtaggtagtt ttaaatatat accaattatt taaaacttaa attgatataa gcataactaa
     3781 cttgtatgag ttaatataag tatataattt aatattattt ttaacccaac cttctaatta
     3841 ttttaaattt aagatacaat gattagaagg aattttttaa gtttcttaga atactgtaaa
     3901 ttatataatc ttgataaaaa gtgttatact atatataatc taaataaaaa atagtacaaa
     3961 aaggagtatt tatgtttgga tatgttaaaa taaacaaaat ggatttaaca tttagagaat
     4021 atgattatta caaagcttac tattgtggac tatgtaaata cttaaaaaga aatcatggag
     4081 aaatctctag gttttctttg aactatgata taacattttt aattgtttta ttaacagcag
     4141 tatataaccc agagtctata agtacagagg aagtgtgtat agtaaatcca tttaaaaaga
     4201 aaaaggtaat tacaaatgat attacagaat atgcagcaag tatgaatatt cttttaacgt
     4261 attataaatt ggaagataat ttaatggatg ataaacgcat aaaagataaa ttggcatatt
     4321 atatatataa aaacaaatta aaattagcat atgaaaagta tcctgaaaaa gcagagtata
     4381 taaaacaaca actaaatgaa ttaaataagt tagaaaaaga taaaaatata aatatagatg
     4441 aagtatctag tatatttgga aatataatgg gtgaagtatt tgtctataaa aaagatgaaa
     4501 atgagagaaa cttaagaatg ataggtttta atataggtaa gtatatatat ctactagatg
     4561 cctatgaaga tttagatgaa gattttaaga aaggaagata taatccattt atagagtata
     4621 ttgataaaaa tgatgagtta aaagaaaaag taaaaaaaat aatagtaaca tcattaggat
     4681 ttttagctag gggaattgat aatctaaatc taaataaaaa tgttggaata atagaaaata
     4741 taatttattc tggtgtttat ttaagatata ttaatatatt agaaagtaga ggtggaaaga
     4801 atgtacagta atacttataa taatgaaaat tttatgagag caagatcatt catagaaaat
     4861 agtgaattta aaaaagcata tgatttttta aaaactttga ctgacaaatg tgcagagtgg
     4921 tattatttaa ctggtttttc tgcaatgaac ataggatatt atgaagaagg tgaagacttc
     4981 ttaaaaagag caaaatttat ggagcctgaa aatagtgaat atagtgatgc acttagaagt
     5041 tatacacaat atagaaatga ttatagcaat agggctgaca attataatag aagaagacgt
     5101 aatgatttag atggatgttg ttgctgttgc tgtgatgatt gctgttgttg tctaggtgac
     5161 gattgttgtg aaaattgtgc taaactttgg tgtttagatt catgttgtga atgttttggt
     5221 ggagatttaa taacttgttg ttaggatgat aaatatgagt ataaaagttg cttatggagg
     5281 agttttatta gcattaaatg tgatattgtt gactcttaca aacattattc ctgtaaatac
     5341 attgtttatt atggggttag catcactttt agtatcaatt gtaataatgg aatggggctt
     5401 taagagtgga attgcttttt atataggttc tatagtttta ggatttatag tgatggcaag
     5461 taagtctcaa tggattgtat attcacttac ctttggaata tatggtattg taaaatatct
     5521 tatagaaaaa gataggtcta tatatattga gtattttatg aaattagtat ttgcaaatat
     5581 aatgatactt atattgtatt ttttattaag aacaatagtt tatattccaa taaacatatt
     5641 tattataggt tcatttgaga tagcctttat agtatatgac tatgtttaca gttcttttat
     5701 aggttattac aataatagat taagaaagat gctatttaaa aaataatagt tgatttttat
     5761 atttaatttt aatgaaattg tagtttagat aagtgtaaat gatatatatg aatatacatt
     5821 aaagcataaa gctaaattta atatattctt agtgcaacat ataaaagctt tgatttttag
     5881 tcaaagcttt tatatgttag atataaatat tagatataat ataagttttt aaaatagata
     5941 gtataaatta aatatccact atatttacat ctgtagatag agaataaata atatcttttc
     6001 tttctactat tatctcaaca tttttagaaa tttcctcatc aagtgatatt tgagataaaa
     6061 gttcctttgt tggatttatt gctattggat ttccaactct ttttagcata tttatatcac
     6121 cattagtatc tccataggca tacgagtttg ataaatctaa atcatacttt acaacaaaat
     6181 cgtttatagc attattctta cttatagaat cccacattgg aataactgtg cctgtaaata
     6241 tattgttttc aaagacataa tcacttccta gaaaatcggt tacattatat ttttttgcca
     6301 ttttttcaac aagaaatcca gggctaccag atataaatat aactatgtgt ccttgattta
     6361 gatgccactc aatccttgaa cgtgtgtatt tataaactct atctgctttt aatttaatta
     6421 cttggtcact tgtaaagttg atacaggttt tatctaatcc ttttagtgaa tcaacataaa
     6481 ggtcacatat ttctaataga taatcatcat aattaccttg tcttttatcc caatccataa
     6541 aagtgtctct agcatgttta taccaatttt tttggtcaat tatatcgtat ttaattaatt
     6601 ttttaaaatg ttcaaccata agagagtctc tataaagagt accatctata tcaaaaaaag
     6661 ctgctatatt tttcataaga ccacctcaat taatcatttc cttatattat attataaatt
     6721 gtatctaagt tgttaaaata tatgtataat aatttatata ttaaaaaaca agtattttag
     6781 agtttaaagg atgtgactat atgaatttta attttgcaaa ggctacaaat tctagactta
     6841 tgggcagttt aggtcttata ataaattgga tagatgatga aaacaatcac ttttgtcaat
     6901 attttttatt agatgcagag ggattaggat tagcggatta tgtgagttta aataacccaa
     6961 cacaagaaga agcttatatg gaagaagaaa gattgatggg aggatttggt tcagatagag
     7021 ttgaattaac gaaggatgaa tctttatttt tagtatctta ttttggtaat aagaatcttt
     7081 attatgataa gctattgcca ggagataaat gtgagtatat tgatattata aaaaactata
     7141 aaacagattt aactatagaa aaattataca ataaaatatg taaaagagtt gacgaggaag
     7201 ttgaatttat aaactatatg accatgagat ttatagcatg ggatagagaa agtttaaagt
     7261 acttttctgg aagtgacgag attgcaaata tgcacattac taatataaat ggaacattac
     7321 ttaaaaacgt ggtaagtgat aaaggtcaag gcagatatat aagtgaagct ttatatgaag
     7381 ataatgatgg atattatata tgtaaaattg ctttttgcat aagtaagtgt tatgaaactg
     7441 gttttagaat taattctcta ttagtgacag ataaagaacc tatgtatgat tttgaggtat
     7501 ttgatgagat ttctaaaagt gaatttgtaa gtgtttacag tgtaaattca cctgaagaat
     7561 ttgcaggagt attttataga gaaaatccat ttctgttaaa aagtaatatg aatgaaggta
     7621 tattctttac tagatttaat tttaataatg accatgtaaa agaaaatgta tatataataa
     7681 ataatgatat gaaagctata tattacttaa tgggaaataa gttttttatt ggtacttata
     7741 acgaaaatga ttgcaattat atcaatgaaa taagtcaatc taattatagc gactatatta
     7801 aatttgaaga gaaatttttc tttgagcaaa atgcattata tgattttgca gaatcaggtt
     7861 cattagattt tgatgatttt ttggaataat aaatttagac attatttaag cgagattaac
     7921 tgaatttaat ctcgcttaaa tttatttcta taatattggg ggaatattat gaggaataca
     7981 tttattattg tggtcaatta ttaaaatttt atacagttta ttataaaaaa tttatatttt
     8041 tctttatgtt gtttgccaat attagtctca aatacgagta ccatactatt ctgaatcacc
     8101 aacacaagag agcattttta aatgaaaaag tcagataaaa ttaaatttta tctgactttt
     8161 tctatgttta tcttattaga ttatgttaat atctgttgac cgaataagcg catattcagt
     8221 caactttcta tacctataat aatattgggg gaatattatg aggaatacat ttattattat
     8281 ggtcaattat taatgtttta tacaacttac ttaaaaaaat atatattaca tttcttcctc
     8341 atcaaattcc tcaatattag tagcttgtgg tataccttgt acagacatat tactagaacc
     8401 ataaggagta ggatatatat taggaggcat agtactaggc ataactacag gcataccttg
     8461 cattgtatta tgacatataa ctcctggtat catcataagt acattaggag gcatattttg
     8521 attacatgag aagttaggaa tacccatagt attgttcata ttatacccca tattactgtt
     8581 catgttatta ggcatacctg tattaccata catattgtta ttcatattgc cagacatatt
     8641 gttaggcatg cccatattac tattcatatt attagacata ttgttaggca tacccatatt
     8701 actattcatg ttattaggca cattgttagg catgcccatg ttactattca tattattagg
     8761 catattgtta ggcataccca tattactatt catgttatta ggcatattgt taggcatacc
     8821 catattacta ttcatattgt tagacatatt attaggcata cccgtattat tattcatgtt
     8881 attaggcata ttatttgtag cagatgtatt gtaattcata tttccggatg tattatttgt
     8941 gttaggcaag ttgtttggag aattactact tttaggataa tgatttacat tattaggata
     9001 taggttattt atgttctggt tattaggtat ttttgtataa ccattagaaa tttgagggtc
     9061 atttctcaat tcatcttggg gaatttcttc attagtaata taatctgcat agttcgctgg
     9121 gtctaagtaa ggcatgtaac tagtatctaa atcctttttt ttcttgttac tcatattaaa
     9181 cttgcacctc ctagaaaact ataaaaaagt ttacaaaaac ttattctaat aatatatata
     9241 ttaaaaaagt ttataatatg tgcattctat ttatattaaa ttaataaaaa ttattgttgc
     9301 aaatttagaa atgataacat ataatgaaca tatgaacagt tattcatata ttaaaataat
     9361 ggaggattaa aaatgagaga agagataaat gattgtaatt gtaatatagt acatgaagaa
     9421 atagtaacag aggctaaatc aactatgcca gatgaagaaa tgttatatga tttagcagaa
     9481 ttatttaaag tatttgggga tactactaga gtaaagatat tatatgcatt atttgcaaat
     9541 gaaatgtgtg tatgtgatat agcgagttta ttaaatatga ctcattctgc aatatctcat
     9601 caacttagag tattaaagca ggctagatta gtaaagttta gaagagaagg aaaaacagtg
     9661 tattactcat tagatgatag tcatataagt cagatatttg attgtggatt aaatcatatt
     9721 agagagactt ataaatgata aatgagagga gtgaatatat atggaagcaa ataattcaat
     9781 taaaaaagaa tttatactag gaggattaaa ttgtgctcac tgtgcagagg agattaataa
     9841 taaagtttct aaactacaag aagttaaatc ttccaattta aattttataa ataaaaaact
     9901 tacagtaaat ataaaggaaa gttttaatga ggatactact atagaaaaga taatagatat
     9961 aatagattca acagagccag gtcttgatat acaaataagt tctaaagaaa atgcagctag
    10021 taaaacatca attaaaaaag agcttatatt aggaggatta aactgtgctc attgtgcaga
    10081 agagattaat aacaaagttt ctaaattaaa agaagttgaa tcttctaatt taaactttgt
    10141 aaataaaaaa cttacagtaa atataagtaa taactttgaa gaggatgatg ttataaataa
    10201 aataaaagaa ataataaatt caacagagcc agggcttgat atacaagtag ggtctactga
    10261 caaagtaaaa gggagaacta ctgaaaaatc aggagctgta aatgatacaa ataagaaaga
    10321 attaattcca cttataattg gagcacttgt atatatattt ggtatatatc aaacagctac
    10381 aggttatgaa agtcagttta gcaatatagt atttatagtt gcctatgtaa tagtaggtgg
    10441 tgatgtgctt ttaagagcaa taagaaatat atcaaaagga agagtatttg atgagaactt
    10501 tttgatggct ctagcaacag taggagcatt aacaataggg gagttatcag aagcagtagg
    10561 ggttatgctt ttctataaag taggagagta tttacaaggt gttgcagttg gtaaatccag
    10621 aaagtctata acttcactta tgcaaataag accagactat gcaaatctaa aagttaattc
    10681 tgaagttaag gtggtgtcac cagaagaggt aaatgtaggt gatataatag tagtaaaacc
    10741 tggtgaaaaa gtaccattag atggtgtagt agtagatgga gtttctatgt tagatacttc
    10801 tgcactcaca ggagagtcag tgctgagaga agttgaaaaa ggagatgaaa tcttatcagg
    10861 tgttataaat aaaaatgctt tattgagtat tgaagttaca aagagttttg gagaatcaac
    10921 agtttctaag attcttgacc ttgttgaaaa ttctagcatt aaaaaatcaa agacagaaaa
    10981 ctttatatct aagttttcaa gatactatac tccaatagta gtaatagctg ctttattaat
    11041 agcatttgtg ccaccacttg taatctcagg tgaggtattt agtgattggt tatatagagg
    11101 attgatattc ttagtcgttt cttgcccttg tgcattagtt ttatcaatac cacttagttt
    11161 ttttagtgga attgggtttg catctaaaaa tggtatcctt attaaaggaa gtaactattt
    11221 agaagcttta agaagtgtag atacagtagt atttgataaa acaggaacac ttactaaagg
    11281 tgtgttcaat gtaactaagt taaatcctga aggtatatca gaagaagaat tattagaata
    11341 tgcagctatt gcggaggtaa attcaaatca tccaatagca aagtccatat taagttacta
    11401 taataaaaaa attgatttag atacaataga cagttatgag gaaatagcag cttatggaat
    11461 tagagtaaag cataatggca attttatatt agcaggtaat gagaaactta tgaaaaaaga
    11521 aaatatatct tattcatcag ctaaagaagt aggcacagta gtctatattg ctgttgataa
    11581 agtatataga ggttatatag ttatctctga tgaagtaaaa gaagatagta aaaatgccat
    11641 aagaagctta aaagaaatag gggtaaaaga agttgtaatg ttaactggag ataatgagaa
    11701 agtagcaaaa aatattgctc aagaattaga attggataca gtttactcaa atttacttcc
    11761 taatgaaaag gtagatagat tagaagattt atatgaagga agaactgaaa aagagaaaat
    11821 agcgtttgta ggtgatggaa taaatgatgc tccagtatta gctcgtgctg atgttggtat
    11881 agctatggga ggcttaggtt cagatgctgc tattgaagct gcagatgtag tgcttatgac
    11941 agatgagcct agcaagatat ctaaagcaat tgaaatagca aacaagacta ataagatagt
    12001 gtggcaaaac ataatatttg ctctaggagt aaaaattata gttatgatac taggtgctgg
    12061 aggagtagct actatgtggg aagctatatt tgctgatgta ggtgtggcac ttatagcagt
    12121 agtaaatgct atgagagcta tgagataagg gataaatgta aatccatact atttaaaaaa
    12181 cttataatta tatattgaag tatgtaataa tgaaaggtat caataagttt cttttaactt
    12241 tattggtacc tttttattgg agcgctttgc atatataaat ttatattcat tatccattat
    12301 gtttatttaa tgtatttatt tagtgtagag cttttatatt gtaattttaa aatttagaca
    12361 tagaaggttt attaataagt ttatattaag tttaaattag
//
LOCUS       DAEORZ010000119        12354 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEORZ010000119 DAEORZ010000000
VERSION     DAEORZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.120"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            19..1098
                     /locus_tag="KRH39_002896"
     CDS             19..1098
                     /locus_tag="KRH39_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG8512783.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEVQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            1211..1810
                     /locus_tag="KRH39_002897"
     CDS             1211..1810
                     /locus_tag="KRH39_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512784.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(1997..2869)
                     /locus_tag="KRH39_002898"
     CDS             complement(1997..2869)
                     /locus_tag="KRH39_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8512785.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            3286..4689
                     /locus_tag="KRH39_002899"
     CDS             3286..4689
                     /locus_tag="KRH39_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512786.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            4716..6260
                     /locus_tag="KRH39_002900"
     CDS             4716..6260
                     /locus_tag="KRH39_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG8512787.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLTKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            6291..7682
                     /locus_tag="KRH39_002901"
     CDS             6291..7682
                     /locus_tag="KRH39_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBG8512788.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            7875..9371
                     /gene="abfD"
                     /locus_tag="KRH39_002902"
     CDS             7875..9371
                     /gene="abfD"
                     /locus_tag="KRH39_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBG8512789.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            9506..9781
                     /locus_tag="KRH39_002903"
     CDS             9506..9781
                     /locus_tag="KRH39_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512790.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            9805..11112
                     /locus_tag="KRH39_002904"
     CDS             9805..11112
                     /locus_tag="KRH39_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG8512791.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQMNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            11132..12250
                     /locus_tag="KRH39_002905"
     CDS             11132..12250
                     /locus_tag="KRH39_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG8512792.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
BASE COUNT         4727 a         1379 c         2388 g         3860 t
ORIGIN      
        1 ttatttggag gtaaaatcat gagcagagta aaaaatgtag tagaattgtt ggaaactaaa
       61 ggtgttgatg cactatatct aacaaagaaa actaatgtaa actatataag tggttttcct
      121 gatgaagaag catatgcagt gatttgcaaa gatggaaact ttttagttac tgatagtaga
      181 tatatggagc ttgcagaaaa ggtttgcaaa gattttgaaa ttataaattg gcataatttt
      241 gatagaagtg tggcaaaggc agttaaaagt gtatgtgata aagtgggtat aaagaaactt
      301 gggtttgaaa gaactaatat agtgtttgat aaatatgaag aacttaaaaa tctgattgag
      361 aaggataatg gagaattaat acctacagaa aatattgtag agactttgag atatgtaaaa
      421 gacaaagatg aaatcaaaaa tacaagaaaa gcttgtgaaa tagcggataa ggcattagaa
      481 gaattgattc cacatataaa agctggagtt agcgaaattg aactggctac aaagttagag
      541 tactttatga aaatgaacgg agcacaaaat atagggtttg aaactatact gatatcagga
      601 gcaaagactt cattgcttca tggtaagcct agtgataaga ttatagaaaa aggtgatttt
      661 gtacttatag actatggcgc tatgtataat ggctatatat ctgatacaac tagaactttt
      721 atagttggag gagcttctga aaagcaatta gaaatatata atttagttaa ggaagttcaa
      781 aatgtagggg ttgagaacat gaaagctggt gtacatgcga caattccaga tgctgagata
      841 agaaaagttg tcaaaaaata tgaagactat tactatcaag gtataggaca tggagtcggt
      901 agagatgtcc atgaagaacc tttcattggt aattatggtg ataaaatcat agaagaggga
      961 tgtataataa caatggaacc aggtatttat ttccctggat ggggtggagt tagaatagaa
     1021 gatactgttt tgattactaa aaatggtcca gaaagactta ctaaattccc taaagatttg
     1081 atgattttag acaaataatt taatttatat atattttttt acataaaata taacataatc
     1141 tcactattaa gctctattta catattatag taaagagggc ttaattttat tgtgagaggt
     1201 gatagtatat atgtataaaa aacataaatt tataaaaaat ataaaaaaag cctggtataa
     1261 aattaaatta attgatagat gccttataat tgtaatggca atattaatgt ttcaatctat
     1321 atataattta tttgttaatg aagtaaattc acaagatact acaactattg atgtagtagt
     1381 aagaaccact tcagcagcta tatttgggta ttttttaagt gctaatttta taaagagacc
     1441 tttaaggaag tatacaaata ctgatgtatc ttcaaatctt tttatagata ataatttatc
     1501 cccaaaaggt caagagtcta atcaaaataa tataatgaat gtcaaaaata ccattggatt
     1561 tacctcagaa gctgataatt atgaaaagaa aatacctatt aataataatg aaggttttga
     1621 agaaggagaa actagtgaat tgcaaattat tatagctact gtaatttgta tagtagcatt
     1681 aacagtactt tttgttgtaa gaaattttac aactacaaca tcagcttctt taggtactat
     1741 ttcacagatg agagattttg tctcaggatg cgtgggtttt ttgctaggtt gtccaagtaa
     1801 acctgattaa aaaagtatta tcaataaaag gtattttaaa aagtatcagt ataataatat
     1861 ttagatttaa atgtatacaa ttataattct gtaacattct atattgtatt tttatactga
     1921 tactctaaac tatactattt attaaaatat tttaacattg tatttaaaca aaatattata
     1981 tctgtataat ataaatttag tttatatgaa tagatgccat atactcaata aaaagtttca
     2041 tactatgaga tatgtattga tttcttctcc aaagaacccc aatatttgca gtaattgcac
     2101 gttctaatgg tattgaacag agattttctg tattttgaaa tatttcttta tacaatattg
     2161 ttgaagccaa gtcattttct agtaaataac gtatggtaga aagttgattt gagtgtaaaa
     2221 ttatatttgg cttagctttt tcaagtcttt ttgtaataat cttatagtga taagaacctt
     2281 cagataagat tacaaaaggt acatttttta acatataatt cgatataatg ggatgttttg
     2341 ccaattcatt tttgggattt gtaactaagt ataattctgt tttatagatt gtctttgaat
     2401 ctatatcatc ataaatatca ctgtcaatta taccaataca aaaatcaagt gtagattctt
     2461 caatcatttt agctccaaca aatgttggaa cttcaaaaat tttaagctct atatctggat
     2521 aaattacatt aaacctagga agtatttttg aaagcaaaaa cgtacctaaa actgtaggaa
     2581 tacctatttt gagagaagct tttctctttt ttcctaaatc aagtgcgtca cattgaaaat
     2641 cctcaaattg ttttataaaa tgttttgact tgtctaaaaa aatttttcct tcaggcgtta
     2701 actctatttt attacctatt ctgtaaaaaa gttcaaagcc aagttctttt tctaattctt
     2761 taattgctaa tgttatggat ggttgagaaa tatgaatact ttccgcagcc ttagtcatgt
     2821 ttttatattt acagacagtt tgaaaatagt ataaatagtt tatattcatt tggtattagt
     2881 tcctttcatt tacatattag tgacaagtat ataattaaca atagcataag ttttgtatat
     2941 tgttgtaaaa aaatagaata gttttaaact atcatagcat aagtttttga tattttcaat
     3001 ctattaagaa tagtgctatt attaaattaa caaaagagaa tagctggtat ggtttagagt
     3061 aaatcaatct atattacagt tatatcaacg aatatcaaaa aattatctat taaaaaataa
     3121 aaattatttt tacaattaaa ttgttaatta aatcataaag aaaaaagtga tattaacaag
     3181 ataaaatttt tttaatcaga aatgttaatt taataacaaa atagagtatt ggggagaaag
     3241 gagaaatata ttttttaata atatttgtta atttaataac gaaaaatgaa agggagtgat
     3301 ttagtgaagt ttgaattgat ggatttttta atgaatcctt ttgtccttat gtttgctgca
     3361 gtaattacag gaattttatt tggaaaaatt aaatttggga agtttaattt tggggtatca
     3421 ggagcattat ttacaggatt atttattgga tggcttgcat atagcttggg gaatctaata
     3481 atagcaaaag gggaaacagc agcaggatat aaggcagcaa ctgtaatgat ggggaatgga
     3541 attatttctt ctgacttctt tgatttcttt ttaataatat ttgttgcagc agttggtcta
     3601 ttggcagcaa aagatatgaa agctgtatta aagaagtatg gagcaagatt tgtaatttta
     3661 ggagttctta taacttttat aggtggctct atgacttatg caatgacttt actatcaagt
     3721 gataaaggaa gtagtgctta tgaagtttct ggcgtttaca caggagcatt aacaagttcg
     3781 cctggattag ctgctgcttt agaaacagct ggtaaacatg cagaagatgt atcaaaagaa
     3841 tttgaaaaag catcaataaa agataaaaaa gaaattttaa aagtagtcga cccagaaggc
     3901 aaattggatg tgaatacaac tacatcactg actcaagagc aaatagataa gtatattgca
     3961 tatgctgaag ctggtgttgg tataggtcat gcagtcgcat atccttttgg agttttaata
     4021 gtaatcttag gtgtaaattt ccttccaaaa ttatttagga tggacctaaa agaagaaaga
     4081 agaaaatatg agaaggaaat gaaggaagca agagatagtg tttctggtaa aacgatacca
     4141 gaagttccat ttaatataat aacatttttc ttgacatgtt tagctggata cttagttgga
     4201 ggaatacatg tatttatggg accactaggg tacttcacac taggtgcaac aggaggttca
     4261 ctaattgtat ctctagttct tggatatata ggtaagatag gggtagtaaa tttcagaatg
     4321 gaagaaaagg ttttaaatat tttaaaacaa atagggctag tatttttctt agcaatagtt
     4381 ggattaagat atggtggaaa agtagttgat tcaattatga catctggtat gcacctagcc
     4441 ttagtagcaa tagcagtagg tgttacagct atgatgatag gattcttagt tggcaaatac
     4501 gtatttaaat taaattggat tttattatct ggagctgtct gtggtggtat gacatcaaca
     4561 ccaggtcttg gagctgctgt tgatgctctt gatagtgatg acccagcagc aggttatggt
     4621 gctacgtatc catttgcatt attaactaaa gttatattgg ttatagtttt acacaaactt
     4681 ccaatgtaaa taagatttta tatgggggga tatttatgaa taatgaagga gcatttgaag
     4741 aattaagtgg aagattaaga aataaagaat tgtgtgattt aataatgaat gcagatgaag
     4801 ctgctaaaat tatagaggat ggcatggtta ttggagttag tggatttaca ccttcaggct
     4861 atccaaaagc agtaccatta gcagtttcag aaagagctaa aagtggagaa aagataaaat
     4921 taaccgtata ttcaggagca tctttaggtc cagaagttga tggagcatgg tcagaagcag
     4981 gtataattga aagaagactt ccttatcaaa ctaattcaat acttagaaat aatattaata
     5041 aaggtgttgt tgattatata gatatgcacc taagtcattc tactcaattt ttaaattatg
     5101 gaactatacc taaagtagat gtagctatag tagaagcact tgcaataact gaagaaggga
     5161 atattatacc aactagtggg ataggtaatt ctccatcttt tataaaaagt gcagataaag
     5221 taatagtaga gataaatctt acaaaaccaa tggaaatgga aggtatggca gatatatata
     5281 ttacggaaaa tccaccaaat agaaaaccaa tagaaataaa tcatcctaag gatagaattg
     5341 gaacaacata tataccttgt ggtttggata aaattgcagg agtagtaatt accaatatgc
     5401 aagataagac ccgtccattg ggagttgtag atgaagcttc gaaaaaaata tcaaataata
     5461 ttattgcttt tttaagagaa gaagtaaagg caggcagatt atcaaagaat ttacttcctc
     5521 ttcaatctgg tgtaggaagt gtagcaaatg cagtattata tggattatgt gaatctgaat
     5581 ttgaaaattt aacttgttat acagaggttg tacaggactc tatgttggat ttaataagaa
     5641 tgggtaaagt tacaatggca tctactacat ctgtaagtcc atctccagaa ggattaataa
     5701 aatttgaaaa agatattgat ttttttaagg ataagattat attaagacct caagaaatta
     5761 gtaacaatcc agaaatagcc agaagaattg gtgtaattgc aatgaacact gcaattgaag
     5821 ttgatattta tggaaatgta aattctactc acataatggg gtcaaagatg atgaatggaa
     5881 ttggtggttc tggtgatttt gctagaaatg gagcaataac tatatttagt actgagtcaa
     5941 ttgcaaaaaa tggtgatata tcatcaatag tacctatggt atctcacgta gaccacactg
     6001 aacatgatgt aatggtaatt gtaacagaac aaggatatgc tgatttaaga ggtcttgcac
     6061 ctagagaaag agctataaaa ataatagaaa attgtgccca tccagattat aaagaacaac
     6121 ttagggatta tttaaataga gcttgtaaaa gtggagcaaa acaaacacct cacatattgg
     6181 acgaagcttt atcatggcat agtaaattta tgagtacagg tacaatgaaa aaggctgaaa
     6241 cttttaaaag tgcattataa aatatttaat aaattggagg tagattttaa atggaaaaag
     6301 cagtggaaaa ctttgaagat ttaagtaagg aatacattaa tggttatatt gagagagcaa
     6361 gaaaagctca aagagagttt gaatgttata ctcaagaaca agtagataaa atagtaaaaa
     6421 tagttggaaa agtagtgtat tataatgctg aatatttagc taaacttgct gtagaagaga
     6481 ctggaatggg agtatatgaa gataaggtag ctaaaaataa aagcaaagca aaagttatat
     6541 ataacaattt aaaagataaa aaatcagttg gtataattga tatagataga gaaactggaa
     6601 taacaaaggt agctaaacca gttggagtag tagcggcaat aactccttgt acaaatccaa
     6661 tcgttacacc tatgagcaat gcaatgtttg cacttaaggg aagaaatgca attattatca
     6721 cacctcatca taaagcaatt ggatgtagta caaaaactgt agagatgatt aatgaagaat
     6781 tagaaaaaat aggagctcca gaaaatttaa tacaaatact tgaccaacaa tcaagagaaa
     6841 atacaagaaa tttaatttca tcagcagatg tagtaattgc cactggagga atgggaatgg
     6901 taaaagctgc gtactcaagt ggaaaaccag cacttggtgt aggggcagga aatgttcaat
     6961 gtatcataga tagagatgta gacattaaag aagcagtacc taaaatcata gcaggaagaa
     7021 tttttgataa tggtatcatt tgttcaggtg aacagtcagt aatagtagca gaagaaatgt
     7081 ttgataaaat aatggatgaa tttaaaaata acaaaggttt tatagttaga gataaagtac
     7141 aaaaagaagc tttcagaaat gctatgtttg taaataaatc tatgaataaa gatgcagtag
     7201 gtcaatcagt tcatacaatt gcaaaaattg caggtgttga gataccagaa gatacaaaaa
     7261 taatagtaat agaagctgat ggaccaggag aagaggatat tattgcaaaa gaaaaaatgt
     7321 gtccagttat atctgcatac aagtataaaa gttttgaaga aggtgtagct attgcaaaag
     7381 caaatttaaa tgtagaagga aaaggacata gtgtatctat acattcaaat acagttaaaa
     7441 atatagaata tgcaggagaa aatatagaag tatcaagatt tgttattaat caatgttgtg
     7501 ctactagtgc tggaggaagt ttctttaatg gtcttgcacc tactaatact ctaggttgtg
     7561 gttcttgggg aaataacagc atctctgaaa atcttgatta taagcatcta attaatattt
     7621 ctagaattgc ttattatatg ccagaaaatg aagtccctac agatgaagaa ctttggggat
     7681 aaaatttaga caaaaaatat tcattctaaa ttaaagtagg aatatatttt aatttagaat
     7741 gaatttaaga cataaattaa atttaaatta gtttattgga ctaaatttaa aatattaaga
     7801 gacatataga agaaggaagt attataattt aaaataaata aaaaaatcta taaaagagaa
     7861 ggagtggttt tattatggca ttaatgacag gagctcaata tattgaaagt ttgagaaaat
     7921 taaatactaa ggtttatatg tttggtgaag aggttaagaa ttgggttgac catcctatga
     7981 ttagaccatc tataaattgt gtagcagcaa cttatgactt agctcatgac ccagaatatg
     8041 cagatttaat gacagttact tcaaatatca caggtgaaaa aataaataga tttggtcatc
     8101 ttcatcaaag tgttgatgat ttgataaaaa aagtaaaaat gcaaagacta tgtggtcaaa
     8161 aaacagcttc ttgtttccaa agatgtgttg gtatggatgc gtttaatgca gtgtattcaa
     8221 caacatttga gtgtgataaa gcccatggaa ctaattatca tgataatttt gtaaaatact
     8281 taacttatat acaagaaaac gatttagtag ttgatggagc tatgactgac cctaagggag
     8341 atagaagtct atcacctagt gctcaacctg acccagatat gttccttcat atagtagaaa
     8401 gaagagaaga tgggataatt gtaagaggag caaaggcaca tcaaactggt tcaataaatt
     8461 cacatgagca ccttataatg cctactataa gtatgactga agcagataaa gactacgctg
     8521 tatcatttgc agttccttca gatgctgaag gagtgtttat gatttatgga agacaatctt
     8581 gtgacactag aaaattagaa gaaggtgcag atgttgattt aggaaataag gagtttggag
     8641 gtcaagaagc tttagtagta tttgataatg tatttgttcc aaatgataga attttcttat
     8701 gtggagaatg ggatttctct ggtatgttag tagagagatt tgcaggatac catagacaaa
     8761 gttatggtgg atgtaaagta ggagtaggtg atgtaataat aggagcagca gctttagcag
     8821 ctgattataa tggagcaaac aaagcttcac atataaaaga taaactaata gaaatgactc
     8881 accttaatga aagcttatat tgctgtggta tagcatgttc atcagaaggt cacaaaacag
     8941 aagctggaaa ctatcaaata gatttacttc ttgcaaatgt gtgtaaacaa aatgtaacta
     9001 gatttcctta tgaaatagta agacttgcag aagatatagc tggagggctt atggttacta
     9061 tgccttcaga aaaagacttt aaatcagacc ttaaagttgg aacaagtgga atgactattg
     9121 gcgaagtatg taacaagtat tttaaagctt cttcagtggc ttcaacagaa gaaagaatga
     9181 gaatattaag attcttagaa aatatatgcc ttggttcatc tgctgttggt tatagaactg
     9241 agtcaatgca tggggcaggt tcaccacaag ctcaaagaat aatgatttca agacaaggaa
     9301 acataaatca aaagaaagaa ttagctaaga aaattgcggg aataaaaaaa gaggaagctt
     9361 taaatttata aaaagttgat atataaaggg aaattatata ttaatgtgta ttaattaatg
     9421 aaaaaatatg tagtataaga gatagttata aaattatggt ggtttttagc taagtaaaga
     9481 aatttttaat aaggagaatt tacacatgac ctgtggagtt agattttgtg ggggctgtaa
     9541 ccctagatac gaaagaggga aggctcttac aataatcaaa gagcacttca atggtagggt
     9601 agattttagt atagcacaag agaacgtcga gtatgatttg ctacttataa ttggaggatg
     9661 cgcaactcta tgcgcatcct atcaacaata taaagtgaaa aatgaatgtg ttaagatgtg
     9721 ggattttagc catatagaaa gagttataaa tgtgattgaa tcagcaattt gtgaaactta
     9781 aaaaagctaa atggggggat ttcattgagt tggcaagaat tgtatcaaag taaattatgt
     9841 agtgctacag aagctgtaaa acagattaaa aacggtgata ctgtagtatt tgcacattgt
     9901 gtaggtgaac ctcctgcact agtagaagct atgattgaaa atgctgaaca atataaagat
     9961 gttgagatta aacatatggt aagcttgggt agtggtggat atactgctaa gggaatggaa
    10021 gctcatttta gagtaaatcc aatgtttgta agtggaaatg taagaaaggc aattgaaaat
    10081 ggagatggtg attttacacc tgcattcttc catgaagtac caaagttgct tagagaaaaa
    10141 agattaaaat gtgatgttgt tttagcacaa gtaactccac cagatgaaca tgggtattgt
    10201 agcttaggaa catcagttga ttatacttat gaagccatta aaactgctag aacagtaatt
    10261 gttcaaatga atgaccagtt tcctagaacc tatggtgagg tagtccatgt atcagagttt
    10321 gactatatag tggaaaaatc acaaccttta tttgaactac aacctgcaaa gattggagaa
    10381 gtagaagaag caattggtaa aaattgtgct tcattaattg aagatggttc tactttacag
    10441 ctaggaattg gtgggatacc agatgcagtt atgttatttt taactgataa aaaggattta
    10501 gggattcatt cagaaatgat ttcagatggg actttggccc tttatgaaaa aggtgttata
    10561 aatggtaaat ataaaaattt tgataaagaa aaaatgacag ttaccttctt aatgggtact
    10621 aaaaaattat atgactttgc aaataataac cctgcagtag aggtaaaacc agtagactat
    10681 gtgaatcatc cagcaattat aatgaaacaa cataagatgg tttctataaa ttctgctata
    10741 caagttgatt taatgggaca ggtagttgca gaggctatgg gacttagaca atttagtggt
    10801 gttggaggtc aagttgactt tatacgtgga gtgtctatgg gtgaagatgg aaaggcaatt
    10861 atagctatgc cttcaataac tacaaaaaaa gatggtacag taatttcaaa aatcgtatct
    10921 attgtggatg aaggtgcacc tataacgaca tcaagaaatg atgttgacta tattgtaaca
    10981 gaatacggta tagcagaatt aaaaggaaaa tctcttagag aaagagcaag aaatttaata
    11041 aatatagctc atccaagtgt aagagaaagt ctagcagtag aatttgaaaa gagatttaag
    11101 gagaaatatt aataatagag gggtgttaat aatggaagca cttagagtag ttccaaagat
    11161 attttacttt gatacattta aagaatttaa tgaagaattt aaaataggaa aaaatgattt
    11221 agtaattact aatgagttta tatatgagcc atatatgaaa cctcttggaa tagatacaaa
    11281 tttaattttt caagaaaagt ttggaacagg tgaaccatct gatgaaatga tagactccat
    11341 gacaaaggaa atgaagaaat acaattttga tagaattatt gcatttggag gaggaacaat
    11401 agttgatata tgtaagatat tagcacttga tgttccagaa aagtctattg atttatttga
    11461 aggagaagta tcacctaaaa aagtaaaaga gttagtagta gttcctacaa catgtggtac
    11521 aggtagtgaa gtaacaaatg tagctattgc agagttaaaa tctaaacata ctaaaaaggg
    11581 gcttgcagta gaagaaacat atgcagacta tgcagtgtta attccagaga cagtaaaagg
    11641 gttaccttac aaattctttg ttacaagttc agtagatgct cttatacatg ctattgagtc
    11701 ttatctttct ccaaaagcaa gtccatttac agagatgtat tctctacaag caataaaaat
    11761 gattatggat gggtataaga agatagttga taaaggagaa gaagagagat ttaatcatct
    11821 tagagacttt gtacttgcat caaattatgc aggaattgct tttggaaatg ctggttgtgc
    11881 agcagttcat gcactatctt attcaatagg tggagctttc catgttgcac atggtgaagc
    11941 aaattatcag ttctttactg aagtatttaa gatgtattct agaaaaaaac ctaatggaaa
    12001 aattataaaa tgtacaaaaa ttttagcaga tgcccttgaa tgtgacccta actgtgatgt
    12061 ttatggagaa ttagaaaagt ttttaaataa attaatagct aaaaaagctt tgagagaata
    12121 tggaatggtg gaaagtcaga tagatgaatt tacagatagt acaatagcaa atcaacaacg
    12181 actacttgct aataattatg tagagcttag tagagaggaa ataagagaga tatttgcaaa
    12241 tctttattaa tacaattttt tatttatatg tatagtcaac ataaacaatg ctaattatat
    12301 ttatgtacaa ataaaaataa gggaacttaa attttaatta aagttaagtt ctct
//
LOCUS       DAEORZ010000120        12175 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEORZ010000120 DAEORZ010000000
VERSION     DAEORZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.121"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(56..499)
                     /locus_tag="KRH39_002906"
     CDS             complement(56..499)
                     /locus_tag="KRH39_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512793.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(502..699)
                     /locus_tag="KRH39_002907"
     CDS             complement(502..699)
                     /locus_tag="KRH39_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8512794.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(891..1757)
                     /locus_tag="KRH39_002908"
     CDS             complement(891..1757)
                     /locus_tag="KRH39_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8512795.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(1983..2852)
                     /locus_tag="KRH39_002909"
     CDS             complement(1983..2852)
                     /locus_tag="KRH39_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG8512796.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(3358..3579)
                     /locus_tag="KRH39_002910"
     CDS             complement(3358..3579)
                     /locus_tag="KRH39_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512797.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(4080..5060)
                     /locus_tag="KRH39_002911"
     CDS             complement(4080..5060)
                     /locus_tag="KRH39_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512798.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(5224..6096)
                     /locus_tag="KRH39_002912"
     CDS             complement(5224..6096)
                     /locus_tag="KRH39_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG8512799.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(6260..6811)
                     /locus_tag="KRH39_002913"
     CDS             complement(6260..6811)
                     /locus_tag="KRH39_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8512800.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(6831..7733)
                     /locus_tag="KRH39_002914"
     CDS             complement(6831..7733)
                     /locus_tag="KRH39_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG8512801.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(7748..10120)
                     /locus_tag="KRH39_002915"
     CDS             complement(7748..10120)
                     /locus_tag="KRH39_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBG8512802.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(10341..11030)
                     /locus_tag="KRH39_002916"
     CDS             complement(10341..11030)
                     /locus_tag="KRH39_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG8512803.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(11271..>12175)
                     /locus_tag="KRH39_002917"
     CDS             complement(11271..>12175)
                     /locus_tag="KRH39_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8512804.1"
                     /translation="SIKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSE
                     GYYFDNFVNPKLVGASSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESH
                     MRVKKLYHDINNHIYCIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINE
                     KSEVCLKKGIKFTCSINFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRI
                     KGTITRSFFVLKCENSKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLF
                     ENSIDKFILNIYIPLDQDTDSWGD"
BASE COUNT         3992 a         2143 c         1154 g         4886 t
ORIGIN      
        1 ttaatttaaa aactaattta tttttatata attttctatt ttatatcata acttattaca
       61 actttttatc taatttattg tatacaacaa cactacaaat aaattgaaat aaaataagca
      121 atccaagaac aactaaaacc cacttgctta ttaccattac agaaaataca ctcaatatag
      181 aaacaataaa tataagaatt acaagaacat aggacatgat ttgattagtg atacgtccag
      241 ctttatcacg taacataatt cttctttcat cattcatctc tatcctttga ttttttatct
      301 tttcttcata atctactgct ctttcaggtt tactccaata catatattga agaatcatac
      361 atacacctgg acctaaagca gctccagata atccccaaat aaaagcctca aatgaaaact
      421 cagtaaatgt agccaataaa acaagtaaaa ttccacaaat aaaatatata attccaacat
      481 ataacatatt ctttttcatt tttactcctc ctcataaata aatatttctt caatactcat
      541 atcaaagtat ctagctatct taaaggctaa gatgatagaa ggattatacc taccattttc
      601 taatgaacct atagtttgtc ttgatacttg caaaatagaa gcaagttctt cttgttttat
      661 gcctctttct tttcttattt cttctaatct atttttcaca tctatccccc tttaatatct
      721 caactacgga aagtctactt tccataatta tacaactata ttccatttat gtaaagctta
      781 ctttccatat ttacaaaaaa ataacagact attttcagcc tgtaatttta gaatttcatg
      841 ttgtttatta attttaatgt tatttattaa ttccatatta tttattaatt ttaacttggt
      901 ttctgaattg gaatccaaac ttctgtagta taatcaggag cattgatatc gccattggga
      961 taaagttcaa tatctggtaa attagcatac acatacccag aacttggtaa ccactcagta
     1021 ataattcttt tttgcaattc ctgtattgcc agagcatttg gcattggaga aacacattca
     1081 aatactgccc atgtgcctga tggtattaag tattcatgag tatcttctaa tacaggctta
     1141 tctgttggag cagcaatata ataatctaaa cccttactat ttgtacacac actaactcca
     1201 agcaatccac aaggttcatt atttattaac tctgaaagtt tatcaattgt tccatttttt
     1261 atgcatttag cccacaactt aggaactttc tcaaagcact cttctacaat catgttcata
     1321 ctttctttta taccaacaat acgaaaccct ttcttttcta caatacgata ttgcatttct
     1381 atatttcctt ttacataaat gcttaatgtc atcttaggat atgcttttaa aacaacaccc
     1441 tttgctctag ctgcagatgg agaaatgtta tgtatatttt gaaaagctct attaaaagat
     1501 gttggtgaat cgtaaccata cttctcagat agttctaaaa ttttgatgtt actgttctga
     1561 agttcaaatg cagccttagt catacgtctt cgtctaatat attcagataa tggtacttct
     1621 gcaatatatg aaaacattct ttgaaaatga aaggttgaac aacatgcaat ttttgctgct
     1681 tctgcataat ccactccact ttcgagattt tcttcaatat aagtaatagc ttgatttaat
     1741 ttatttatcc attccatttg atattacctc caagcaaatt atacagttta ataaaataat
     1801 atacctctct attcatgcac aaaattgtaa gcatttaaat tcataagtat ttttcaatca
     1861 atacaataaa tataaaaatt gtattataca ataaaaaagt tcaataacat tatattataa
     1921 aatgttattg aactttttat aaaaattaat ttaagtgtag ttaaattaag tattacacta
     1981 tcttatatat tacaatatcc tactatctta tagtcttctg gcactttata atctttgtct
     2041 ggagtaccta aattccattt taaatcaaac atagttgcac ttattattgt tgcaaaataa
     2101 agcataacta taccaatgtc tattttttct tcatataaat tagtatgacc atcttttcta
     2161 acagctaaaa ctacagtgcc tccatcaact ataaatctcc atggttgtct atttaatgta
     2221 gatggagcta atcttgcata gtgaaatgca tcaagaagtg ctctttcttc taagatttct
     2281 atacttgcaa catttcccca ttcgttcata taaacaacat cttctacacc tagtctagta
     2341 gaccccttat ctgttacaac ttgcatatca gattttgaat aattttgccc agtttttgta
     2401 gcatttaata ctttagcatt acttacattt tctccatatc caatagctat aattcctgtt
     2461 acctctttat ctgaattaat tccaagtttt tcttttacct tttttccatc ttcaaaagta
     2521 acccaacaag aaccaattcc aagttctgtt actttaaaga ttatactttc tcctatgtac
     2581 cctgaatttt caatgtaatt atcttttatg tcagataata ttataatata acttggagaa
     2641 tctatcatat ttcctttgta ccctgctact ttatctaaat tattaaaaac ttcacttcta
     2701 ttcataattc ttacatcaac atctatttct ggaactagtt tcttacaaga ttctgagtat
     2761 ttcttaattt cactaagata ttcttcttta atatctgtgt tcttatattc tctaacagac
     2821 tttctattta atatgattgt tttataatcc ataattttat cctccccaaa ctagtattgt
     2881 cttaattata tacccattaa tatattattt tactcattta tttaataaat aaagattaca
     2941 attttgtaat tattatttat taataatatt ccacttttac attaataatg accagttgtg
     3001 caaaatccat tgccaaccac caaataaaaa catattataa aggtaatata aaataacaaa
     3061 caaatcttaa caaaaaatta aacttttttt acacgaaaga aggcttcccg ttgccttctt
     3121 tatttttata aaaaaataag ctgtttctaa aatgatttca catttttgaa acagccaagt
     3181 tattctataa tgacaactat aatttgagga tgtgagggag agttgtcatt ataaatttag
     3241 ggtaattata ttatgtttta agctagttct ttctatattt atataataca ttttatatat
     3301 gaattttata tgagattggt ataaatgata tatgaaatta catatgttta tattatatta
     3361 aattttatta ttacattttc cacaacaata ctcatatgtc tccacattat ttggaatatt
     3421 tacaaataca aattctttac agttctcaca atactttaat atagatatag ttccacaagt
     3481 gtaacattca aatattacac aatcctcatt ttcctctact atttcaaact ttttactatg
     3541 ttcaccacac tctgggcact tgtgattatt tacattcata aatactcctc cttattagta
     3601 tagttacatg tcaaaaactt aattaatgta ttctatatta cttattatat attctataat
     3661 aaatcatatt ctaatataat aaaacaaatt aaataaaaaa aaaccataaa ttttaaattt
     3721 atggattaat tcttgtaatg ataactctat ttgaggatgt gagggagggt tatcattaca
     3781 atagggtatt atattatggt ttttaagcta gtctttctac acctcatatg atacatatgc
     3841 tatatgaatt ttatatgatt tttacataaa tattttatga aatttaaatt ttttagtcat
     3901 tttgaaattt gtaaaaagtt ctctaaaaat ccacagcatc ataaacagcc aaaatttaag
     3961 taaaacatca taaaaaaatt tgtccaataa ttttaacaag taatctcacc tcattataat
     4021 tattgacaca aacttaaata aacaatctat aaatcttcta taaatcttaa actttccccc
     4081 tattgataca caatttctag tttgtcaatt ttattttgca tttctaagaa aatttcttca
     4141 acagaattgt taaatatatt gcctaaatag attcttttat ctttttgaaa tgaataagat
     4201 tttacactac catcagaaga aatatataaa tgactccact ctgctccata ttttgaaaac
     4261 ctctcctttg tatctttaaa cctcatatct tctattttta ttgctttttc tctgtctcct
     4321 cttactacag tgtgacccct ttttattaaa ctggttattt ttatatcttc ttttaattta
     4381 atacgtgtat catcattttt tgaatatact ttccatatca ctttatgacg ataattatct
     4441 aattccttta ttaagcttct atcaatagta aaactatctt tagttaccac aattaaatca
     4501 aaatggtcta acatatattg aaatatttct ctcatacttt tattttttaa aatatcctca
     4561 ccaataacct ctataaatct acagcctttc tcatacatgc agttcaataa acttgtgatt
     4621 tcagaaaaat aatcatttgt attattatct ccatctattt ctatagtaaa gtacaaagga
     4681 gtaatgtatg atttatttcc tgttatctca aatacacttg atagtgtctg tatatcatct
     4741 tcaaacgtta agtatacatt ctcataaaac ataaaaaatt ccataacttt ttctttcata
     4801 ttatggatat ttctatatct attggataat atatttatta tatcgtcagt agtatttttc
     4861 ccatcacatt cagccagtat aacaatttgc ttccaactta agctatccac atttccgata
     4921 ctttttttat atccatcttc ttcatcataa atagggccaa ttataatgtt ttcttttggc
     4981 cacagatgaa ccctagttcc atcttttaat ttcggatatt tattttttac agactcataa
     5041 aatctacttg tatcacccat gattctttac taccccccat agtttaaata ttctatgcta
     5101 aaaatagcac tttattttta ttaattcaac aattattcaa aattggtcat tatatatgtt
     5161 taaatggatt taacatcaac cttatttata tgttattgat gtttatatat gttatataaa
     5221 atcttatttc tcatacttca atgtaatctt aactatctct ggtttacaat taagcctaat
     5281 taaaactttt gagcctccta agcctttatt taaaatcata gtgctattat ctttttcata
     5341 ttttccccag tcatacttag gaaagaactc tctatttgga gataatacac ctccaacaaa
     5401 aggtattctt attatacctc catgaacatg tcctgctaaa accaaatctg ctccccattt
     5461 tgcatatttt tcaaagaaaa atggtgtatg tgctaaaaga atgttatatt cattttcatt
     5521 aactttacct agattgtctt ctacaaaatt gtcacttagt tctaattttt tgttattagc
     5581 aaataaatat ggatagcatt ccaatggtat aattaaacca tataaattaa gatggctatt
     5641 acctctatat attcttacag attcattttc catatttatt attttctttg aatatagttc
     5701 cttaaaataa tccttatata gatgtttata cttttttatt aaagattttt gttcatgatt
     5761 tccaattata tgatatactt catatttttc aaccaaatta tctattaaat ctaatgcaac
     5821 ttgaaaattt ttattttctc catctactaa atctcctgtt ataaatataa catctgggtt
     5881 taatgattca attttatcta tcaatacctt attattttta tcaaacacct tattatgtaa
     5941 atcactcact tgaactattt taaagttatc aaattccttt ggaatttttt tgttgaaaat
     6001 agtatattct gtcgtttcta tatgctgagt ttcatactta tacagaatat acaataatat
     6061 taatactaat actgtcattg aaacctttat aatcatattc tcttcctctt tattctattt
     6121 tgttaatatt attataatgc ttttatgttt tttttaaaac aataacatca tcacatacat
     6181 ataaatcttt tcgacattat aaaaataagt tatccttttc taaaattttc atttatttta
     6241 gaaaaggata actataaaat tatctataaa cctctatatt gtgaaatata gtttttcctt
     6301 tatttaaatt tttatatata tgctctaaag ttccatcaaa cctaatagaa tgtccagact
     6361 ctaaaatata agttttccca tccacattaa ggtcaagttt accttctatt acgagcacat
     6421 attcttctaa aacatgacta tgtgtagatg aaacatgagt acagttttcc tcaagctcaa
     6481 ttataaaaag ttcaaacctt ctttgagggc taaaagggta tattggatat aacttcatgt
     6541 tccctttttg ctcaacaata ggctctactt cttctttctt tataacctca taggtatttg
     6601 tattttcatt taatagtgta gtaaaattta ttttaaatcc agatgaaatt ttccataaag
     6661 tagatactgt cggacttgat tcacctctct caatctgtcc aagcattgct ttactgacac
     6721 ctgtcatctt tgaagctttg tctaaactta tgcccatttc ttgtctcaat aaccttaaat
     6781 tttctcccac attaggaaat ttattatcca tgttaaaatc ctcctgatag ctatttgtat
     6841 tctatgattt ttactcctgt aagttctact atctctttta attttaacat atgattgtta
     6901 caaatttcat caaattcata tatataatct tcaagcccta atgccttata tttatcgatt
     6961 ccaaatttat gatatggaag taactccatc ttgcctcctg gaacattttg tttaacaaat
     7021 aatgcagtat ttcttatatt ttcttcacta tcatttactc cataaataat tggcactctt
     7081 ataaccatag atttattcaa ctttgaaagc ctacatatat tatctaatat aattttatta
     7141 cttactcctg tatattcttt atgaatatta tcatccatag attttatatc tacaaatatg
     7201 tggtctattt tctcaaatac atcatttacc ttattccaat caaaatatcc acaagtttca
     7261 attgcaatat ttatgccttt atcataaaaa atatcaacta attctcttaa aaaatcaatc
     7321 tgaagtgtag gttcacctcc agaaaaagtt atccctccat tagattcaaa gaaaaatata
     7381 aaatcttttt caacttcctc tactatttct tcaacactca tttcttcggt cattatacaa
     7441 atggcatctt taagacatac cttgatacac tctccacata aatcacattt attatttatt
     7501 tgcgctctat caaatagtga acttatattt tgaggacaaa catctataca tctattacag
     7561 gatacacatt tatctttcat aacacctaat tttactatgt tactccatga atctggatta
     7621 gaacaccact tacattttaa tttacatcct tctaaaaata tagtagtcct tattccatct
     7681 ccatcattta ctgaaaaatg ttgtactttt acaactctac cttttttact cataatctac
     7741 ctcataatta aaaattgcta tgtgaatttc tagctattat agcatcttgc atttctcttg
     7801 ataaattaac aaattgagta ctatatccag caaccctcac taataaatct ttatattctt
     7861 ctggattttc ttgagctttt aatagagtat catttgatat cgtattaaat tgtacatgga
     7921 atcctcctaa agcaaaatat gatttaatca tagatgttag atttctaagt cctctttcag
     7981 tttctaccaa atcttgattc agtcttaaat ttagaagtgt tccaccagta tgaacatcat
     8041 gatttatttt tgatgcagat ttcattgctg ctagcggact tgttgtatca gtaccaacaa
     8101 atggagaaac tccctctgtc aaaggttttg tcgcttttct tccacaagga gttgcaccta
     8161 ctatttttcc agttggtaca tagtttgata tacccataaa tgcactatta aatttagagc
     8221 caaaaatatc cttgtatttt ctagtttcag tataataaaa atcagaaact tcaattgcta
     8281 atgaatctac ataatcatta tcatttccat acttaggaac atccaacgca agttttctta
     8341 attcttcata accttcccaa tcattattta gtgcttttgt taattcttct aacgtagtca
     8401 ccttatcctc aaatactaat ttttttatag ctgccaacga atttgaaact acacctaacc
     8461 ctataccagt taatacagga cctatattat attttgctcc tcctttactt aaatcaactc
     8521 ccttatccaa acatccatct acacaagtag ataaaaatgg tcttggtacc atttctttat
     8581 gaatttgttg agcaacagta gttcctataa cagaactttt tacaagatat gctaattgct
     8641 ttaaaaactc atccttaagt tcttcaaaag aagtaaattc tgtgatattt tttgtatcta
     8701 accccatttt ttctccagtt aatctactct taccttcatt catagcatat tctaaagctg
     8761 ctgcaaaatt gatatttacg gctgaagtcc actgtcctgt ttttctaaaa tgaggaacta
     8821 cacaaccaca gttactccaa tctctagcat cttctgcatc atatccatct tgtaataaca
     8881 tttgtgcccc tgcactatcg ctgtgtatag cagggaaacc agttccttgt tttaccaatt
     8941 tagcaactgc cattacgaaa ttatctggtg ctccttgact cactctaaca cttaatcctg
     9001 gctggtgagt ttttacactt tctgtagcct ttaaacacat atatgagata tcattcgttc
     9061 catcagtacc atttctcttt ttaccaccaa cagtaagatt ttggaattga ttatatcctg
     9121 caaagtaatt tgctgtattt gctgatatag tccataccca ttctgataac ttaatccaaa
     9181 gtgcttctat caattcttga gcttctgatt cagttatttt accttctcta gcatcatttt
     9241 cataatatgg atacatatat tggtcaaatc tacctaaatt taatgccaat ggattttctg
     9301 atagaatacc acctaattga acaaaccata caaattgtat tgcttcataa aaattagttg
     9361 gagggtttgc tggtacattc atacatatct ctgaaatttt tataagttct aactttctct
     9421 tttcatctgt ttcttgctta ctcatctctt ttgcaagatt tgaatatctt tgtgccaatg
     9481 tcataatacc ctgtgcaact atagaaacag atttgtaaaa atctatcttt tcaatatttt
     9541 ctgaaactga tatatctaat tctttaattt ttttatctgc atcttcctta attcctttat
     9601 atccttttac aaacaatata tcttgataat ctggtgttac ttcaccaact gcctgtctcc
     9661 acttagaatc attatctatt attcctgtat ctactaatat cttagctgta tcttctggga
     9721 ttcttgctaa gaaaacttct tccaaagatt ttcccttcca atatggaaat atttcttttc
     9781 ttataaattc tatttgttct tttgatatta catatgggtc ttgagttctt ttatcaaaag
     9841 tatccatttc tttgtctacc cattgccaag aaaactctgg tgtcaatatc cctgttcttc
     9901 taaattttcc tgctgtacct actattaatt cattttcaaa gatttttata ggaagttctt
     9961 tacatgtctc caaaaaagct ttagctcttc ttatacagat agcttctccc tcagtttttt
    10021 tatgtgattc tgtaaaaatt ctagcccttt cacaacatat cataggttta gaattaatat
    10081 attcttgtct aaatgcttct attctattag tttgactcat ttaaaatccc cctttggata
    10141 atatatacta catttttgta tgttataaca tactttaatt atattatact gtcctttagc
    10201 ataaaatgga agcaaaataa cttatttttt ttcatctacc ttgaacacct atatattaat
    10261 actttttttg ttaaatttat aaaatctaaa aaaattttct atgatatatt ttctatattg
    10321 ttatagttat gtctaatata ctaatgattt gaaatatcaa atttgtatac tagacttagt
    10381 tttaaaacct tagttgattg tttaactttg ttaacttcaa catgtatatc cttcatataa
    10441 ggaagctcta caacacctac tcccttatta aagtcttctt caaccataga aaaaatttct
    10501 ttctcagtta agttcgtatc agtcaatgat ttaaataaat tgtatataaa tttaacatat
    10561 ttatcactct cagataactt atcatcttct ggatactctt tagcacagag aatatctatt
    10621 gttacttttc cttctttatc gtcttcagac atagaataga ataaacttcg aatatttttt
    10681 tcaggtggtt tttcagcacc atttatagat actacaaagc taccatctga ttttgagtta
    10741 gtaatactat aaccactgtc tattatagct ttcgaaaatc tttgagataa atcttttttt
    10801 acttcttctc tacctttcgc atcattaatc ttttgtgagt aattattaaa tagctcaata
    10861 gattcatctt ttttatcttc aacttgttct ttcaatgaat ctttactaat ttgttctgag
    10921 ctacttaagt tcttatcaga tacaggagac atacacccta ccatcaatgc taagctaaga
    10981 tttaaagcca caattattgc cactctattt atattccttt ttactttcat acacaatcct
    11041 cccatcacac taaccgctct aaagtattat tcattgcact agaatatatt tatattttct
    11101 ttatattaaa gatttattat tcaatacata tcaccttcct atccaattct tataacttac
    11161 tattagtaaa attataagct ccttaataca gaaataagga tgttatcttt tgttattaaa
    11221 agataacatc cttatttttt tgtatcaata catatgtccc aactggtaac ttaatcgccc
    11281 caactgtcag tatcttggtc caatggtata tatatattta gtataaattt atcgatacta
    11341 ttttcaaata ataattctcc attatatttt tgtaaagaag atttaatact ctgaatacct
    11401 attccatgta cgaacttatc cattttgttt gttaacaata tatttttatt aaaagtaagc
    11461 tgatttagtt tactattttc acatttaagt acaaaaaatg accttgttat agttccttta
    11521 attctaatat atttattaat attttcatcc actattttat cacatgcttc tattgcatta
    11581 tccaaaatat tagaaaatat gcttgacaca tctataggtt ttacaaaatt tacttttgaa
    11641 aaatttatag agcaagtaaa tttaattcct ttttttaaac atacttcact cttttcgttg
    11701 attataatgt ctaatatcat atttcctgta ttatagatat atttaaatgt ttttatttca
    11761 tcttttaaat tgttaacata ttcattaatt tctttgctat tgtttctaag gttgtctata
    11821 caataaatgt gattatttat atcgtgatat agtttcttaa ctcgcatatg agattcttga
    11881 atacttaaat aatgcgcata ttgcatatct aacttatttt ttattaattc agcttgagat
    11941 tttaatctat tctcttttat tatttcttta aacaatagta taagaaatat actactagct
    12001 cctaccaatt ttggattaac aaaattatca aaatagtatc cttctgaatg taatgaccca
    12061 aaccgttcta atttttttaa tacataattt acaagcatat tcattgtatt aactaataag
    12121 gcaaataata aatatacata atttctctta tcttggctaa ttaattttat agact
//
LOCUS       DAEORZ010000121        12126 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEORZ010000121 DAEORZ010000000
VERSION     DAEORZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12126
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.122"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            142..930
                     /locus_tag="KRH39_002918"
     CDS             142..930
                     /locus_tag="KRH39_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG8512805.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            1401..5117
                     /gene="rpoB"
                     /locus_tag="KRH39_002919"
     CDS             1401..5117
                     /gene="rpoB"
                     /locus_tag="KRH39_002919"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBG8512806.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            5159..8644
                     /gene="rpoC"
                     /locus_tag="KRH39_002920"
     CDS             5159..8644
                     /gene="rpoC"
                     /locus_tag="KRH39_002920"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBG8512807.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            8933..9355
                     /gene="rpsL"
                     /locus_tag="KRH39_002921"
     CDS             8933..9355
                     /gene="rpsL"
                     /locus_tag="KRH39_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBG8512808.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            9479..9949
                     /gene="rpsG"
                     /locus_tag="KRH39_002922"
     CDS             9479..9949
                     /gene="rpsG"
                     /locus_tag="KRH39_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBG8512809.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            9998..12064
                     /gene="fusA"
                     /locus_tag="KRH39_002923"
     CDS             9998..12064
                     /gene="fusA"
                     /locus_tag="KRH39_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG8512810.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         4695 a         1530 c         2494 g         3407 t
ORIGIN      
        1 tattttaaaa aaatacacga gtgtctataa ataaaatgta ataaataatt aattaacaaa
       61 tataaatttt aaaataagtt agatatttag aaaaatcgaa atttgtagga tttaaaggac
      121 atattcaagg aggagttaat aatggaaaaa ttacaaggaa aaattgcagt agttactgca
      181 gcaacaaaag gtattggatt agcatcagca gagatattag caaaaaatgg agcaactgtg
      241 tacttagcag ctcgttcaga agaattagct aatgaagtta taaataaaat aagtgcagaa
      301 ggtggttgtg ctaagtttgt ttactttaat gctcgtgaag aagagacctt tacctcaatg
      361 atagaagagg tagttaaaaa agaaggtaag atagatatac ttgtaaacaa ttttggttca
      421 acaaaccctt ctcttgacaa agaccttgta actggggata cagataattt ctttgacaca
      481 gtaaatacta atttaaaaag tgtgtattta ccatgtaaag cagcaattcc tcatatgata
      541 aagaatggaa aaggaagtat agtaaatata tcaagtatag gttcagtatt acctgattta
      601 tctagaatag cttactgtgt atcaaaagca gcaatcaact cattaactca aaacatagct
      661 acacaatatg ctaaagacaa tgttagatgt aatgctgtac ttccagggct tattgcaact
      721 aaagcagcat tagacaatat gtctccagag ttcataaaag aatttttaaa gcatgttcct
      781 ttaaatcgta taggggaacc agatgatata gcaaaagctg ttctattcta tgctagtgat
      841 gactcatcat ttataacagg ggatttactt gaagttgcag gaggatttgg tttacctact
      901 ccacaatttg cagataatat attaggataa ttaaattact aatatgcaat gaaagcttat
      961 gcattagtta tagtagaaat gatattttta taaaggccat agatataatt tatattggaa
     1021 aggtttgagg ttcaaaattc caagttaaat ttatttatgg tctttatttt gtatatttta
     1081 ataaattgta tatatttgta taaatgtaat tatttacaat cattttaata tggaattata
     1141 attaaatagg ggggatggta cttgtataaa attttttata gaaaattaga cctataaatg
     1201 aaaaatatgg attttatatt ggaaaattgt acataataca taatatatgc ttaaaataat
     1261 ttacaaaaaa tcaaaaataa tgcatagttt acatataaga taaaaaatat attgaaaaat
     1321 acttgacatt atgctaaaat tattagatgc gttagaatga actattgttt tatttttaat
     1381 tattgagagg tgaaaagtga atgccacatc ctgtcacgat aggtaaaaga actagaatga
     1441 gcttttctaa aattaaggaa atagctgatg tgccaaatct tatagaaatt caagtagatt
     1501 cctatgagtg gtttttaaaa gaaggtttaa aagaagtgtt tgatgatatt tcaccaatag
     1561 aagattatac tggtaatctt atattggaat ttgtcgacta ttctctagat gataaaccaa
     1621 agtacgatat agaagagtgt aaagagagag atgctacata ttgtgcacct ctaaaggtta
     1681 aggtaagact tataaataaa gaaacaggtg agataaaaga acaagaagtg tttatgggtg
     1741 acttcccttt aatgacagaa agaggaactt tcgttataaa tggagcagaa agagttatag
     1801 taagtcaatt agttagatct cctggtgttt actatgcgga agaaagagat aaaacaggta
     1861 agagattgat ttcatctact gtaattccta atagaggagc atggttagag tatgaaacag
     1921 attctaatga tgtaatatct gtaagagttg atagaacaag aaaacaacca gttacagttt
     1981 tacttagagc tttaggaata ggaacagatg cagagataat tgacctttta ggagaagatg
     2041 agagattatc tgctacatta gaaaaagata atactaagac agtagaagaa ggtcttgtag
     2101 agatatacaa aaaattaaga ccaggtgaac ctcctactgt agaaagtgca tcatctttat
     2161 taaatgcttt attctttgac ccaaaaagat atgacctagc aaaagttggt agatataaat
     2221 tcaataagaa acttgcttta tgttatagaa taatgaataa aatttcagca gaagacataa
     2281 taaatccaga aactggagaa gtatttgtaa aagctggaga aaaaataagc tatgatttgg
     2341 ctaaagccat acaaaatgca ggtataaatg tagtaaatct actaatggat gatgataaaa
     2401 aagttagagt tataggaaat aattttgtgg atattaagtc tcacattgat tttgacatag
     2461 atgatttaaa cataaaagaa aaagtacatt atccaacttt aaaagaaatt ttagatggat
     2521 atagtgatga agaagaaata aaagaggcta taaaatcaag aattaaagaa cttataccaa
     2581 aacatatatt attagatgat ataatagctt caataagtta tgagttcaat atattctata
     2641 atataggaaa tattgatgat atagatcacc taggaaacag aagaataaga tctgtaggtg
     2701 aattgttaca aaatcaagtt agaattggtc tttcaagaat ggaaagagtt ataaaagaaa
     2761 gaatgacggt tcaagatatg gaagctataa cgcctcaagc gttagttaac ataagaccag
     2821 tttcagctgc aataaaagaa ttttttggta gttctcagtt atctcagttc atggatcaaa
     2881 caaatcctct atctgagtta acgcataaga gaagattatc agcccttggg cctggaggtc
     2941 tttcaagaga aagagctgga ttcgaagtgc gtgacgttca ccattcacac tatggtagaa
     3001 tgtgtccaat agagactcca gagggaccaa atatcggtct tataaactct ctaggaactt
     3061 atgcaaaaat aaatgaattt ggatttatag aatcaccata tagaaaattt gataaagaaa
     3121 catcaacagt tactgatgaa atacattatt taactgctga cgaagaagat ttattcgtaa
     3181 gagctcaggc aaatgagcca ttaacagaag atggtaaatt cgtaaaccat agagttgttt
     3241 gtagaactgt aaatggtgct gttgaaatgg ttcctgaaag tagagtagat tacatggata
     3301 tatctcctaa gcaggttgta tctgttgcca ctgctatgat acctttctta gaaaatgatg
     3361 atgccaaccg tgcccttatg ggagcgaaca tgcagcgtca ggcagtgcct ctagttagaa
     3421 gagaagcacc aattatagga actggtatag aatacagagc agcaaaagac tctggagcag
     3481 ttgttgttgc tagaaattct ggtatagcag aaagagtaac agcagatgaa ataattataa
     3541 aaagagaaga tggaaacaga gatagatata atctacttaa gtttaaacgt tcaaactcag
     3601 gtacttgtat aaatcaaaca cctataataa ataagggtga ccagataata aaaggtgatg
     3661 ttatagcaga tggtccagca actgatttag gagaagttgc acttggaaga aactgtctta
     3721 tagcattcat gacttgggaa ggttataact acgaggatgc tatattaata aatgaaagat
     3781 tagttaaaga agatagatta tcaacaattc atatagaaga atatgaatgt gaagctagag
     3841 atacaaaact agggccagag gaaataacta gagatatacc taatgttgga gatagtgcaa
     3901 ttaagaactt agatgacaga ggtataataa gaataggtgc agaagtagac tcaggtgata
     3961 tattagttgg taaagtaact ccaaaaggag aaactgaact tactgctgag gaaagattac
     4021 ttcgtgcaat atttggagaa aaggctagag aagttagaga tacttcactt aaagtgcctc
     4081 atggtgaatc tggtataata gttgatgtaa aagtatttac tagagaaaat ggagatgatt
     4141 tatctccagg agtaaatgaa ttagtaagat gctatatagc taaaaagaga aaaataaaag
     4201 ttggagataa aatggctggt cgtcatggta ataaaggggt tatctcaaga gtattacctg
     4261 aagaagatat gccattcatg gaaaatggaa caccactaga cataatactt aacccacaag
     4321 gtataccatc acgtatgaac attggtcagg ttttagaagt tcatttagga cttgctgcta
     4381 agacattagg atggtatgta gcaacatcag tattcgatgg tgctaatgag tatgacataa
     4441 tggatgctct tgaagaagct gggtatccta gagatggtaa attaacttta tatgatggta
     4501 gaacaggtga gtcttttgac aacagaataa ctgttggata tatgtactac ttgaaactac
     4561 atcacttagt agatgaaaaa ctacatgcaa gaagtactgg accatactca ttagttactc
     4621 aacagccact aggtggtaaa gcacaatttg gtggacaaag atttggagag atggaggttt
     4681 gggctctgga agcatatgga gctgctcaca tacttcaaga aatacttact gttaagtctg
     4741 atgacgttgt aggtcgtgtt agaacatatg aagctatagt taaaggtgaa aatatacctg
     4801 aaccaggaat cccagaatca tttaaggttc ttataaaaga acttcaaagt ttatgcttag
     4861 atgtaaaagt attgacagat gaagaccaag aaatagaagt aagagaatct gtggatgaag
     4921 atgatacaat aggtgaattt gagctagatg ttgtaaatca tatgggagaa gttgaagaaa
     4981 gtaatataat agaagaaatt gaagatgatt ttgcagaaaa tgcagaagat gaggatatag
     5041 agaatttaga agaattcact gaagatgatt tatttgaaga agaaatagat tttgatagtg
     5101 atgactttga tatgtaaatt taattaatgt aaatctcaaa tagaagggag agaactcctt
     5161 gtttgaatta aacaatttcg agtcgataaa aatagcattg gcttctccag aaaaaataag
     5221 acaatggtct aggggagaag ttaaaaagcc agaaactata aattaccgta ctttaaaacc
     5281 agaaaaagat ggtcttttct gtgaaagaat atttggacca caaaaagact gggagtgcca
     5341 ctgtggtaaa tatagaagag ttagatataa aggtgtagtt tgtgatagat gtggagtaga
     5401 agtaactaaa tcaaaagtaa gaagagagag aatgggacat atagagctag ctgctcctat
     5461 gtctcacatc tggtacttca aaggtatacc aagtagaatg ggacttttac ttgatatgtc
     5521 accaagatcg ttagaaaaaa tattatactt tgcctcatat gtagtagttg atccaggaga
     5581 gactggatta aacgaaaaac aattgcttac agaaaaagaa tacagaactg ctcttgaaaa
     5641 gtatggatat acttttactg taggaatggg tgctgaagct gtaaagacat tactacaaaa
     5701 tatagattta gagcaacaaa gtaaagacct aagagcggag ttaaaagata gtacagggca
     5761 aaagaaagtt agaacaataa gaagattaga agttgtagag gcatttaaaa agtctggaaa
     5821 taaaccagaa tggatgattt tagatgcaat accagtaata ccaccagatt taagaccaat
     5881 ggtacaactt gatggtggaa gatttgcgac ttcagaccta aatgatttat atagaagagt
     5941 tataaataga aacaatagac ttaaaagatt attagagctt ggagctccag atataattgt
     6001 aagaaatgaa aaaagaatgc ttcaagaagc tgttgatgca ttgatagata atggtagaag
     6061 aggtagacct gtaacaggac ctggaaatag accacttaaa tctttatcag atatgttaaa
     6121 aggtaagcaa ggtcgtttcc gtcaaaactt acttggtaag cgtgttgact actcaggacg
     6181 ttctgttata gttgttggac cagaacttaa attttatcaa tgtggacttc caaagaagat
     6241 ggcattagaa ttattcaagc catttgttat ggataaatta gttaaagaag gttatgcaca
     6301 taatataaaa agtgcgaaat ctatagtaga aaaagttaag ccagaagttt gggatgtttt
     6361 agaagatgtt ataaaaagtc atccagttct tcttaaccgt gcaccgactc tacatagatt
     6421 aggtatacaa gcatttgaac caatcttagt tgaaggtaag gctataaaac tacatcctct
     6481 tgtatgtaca gcttataatg cagactttga tggtgaccaa atggcagtcc atgtaccttt
     6541 atcagtagaa gcacaagcag aagcaagatt cttaatgctt tctgtaaata atatacttgc
     6601 tcctaaagat ggttcaccta taactactcc atctcaggat atggttttag gttgctatta
     6661 tctaacaata gaagcccaag atggagctaa aggaacaggt atggtattta aagactttaa
     6721 tgaactatta cttgcttatt acaataaatc agttcatcta catgcattag tgaaactgaa
     6781 ggtaacactt gaagatggaa gaagttcatt ggttgaaagt actgttggta gatttatatt
     6841 taatgaaaat atacctcaag acttaggttt tgtagataga aaagaaaatc catttgcact
     6901 tgaagttgat ttcttagcag ataaaaaatc tcttggtaaa ataatagata aatgctttag
     6961 aaaacacgga aatacagaaa cagctgaatt actagattat ataaaagctc taggatttaa
     7021 atattctaca ttaggtggta taacagttgc tgttgatgat atgagtgtcc cagaagaaaa
     7081 gaaagtattt atagctgagg cagaagctaa agttgataag tatgaaaaag catacagaag
     7141 aggtctaatc tctgatgaag aaagatatga aaaagttata gagacatgga cagaaacaac
     7201 tgataaagtt actgatgctc ttatgggtgg actagataga ttaaataata tatatataat
     7261 ggcacattca ggagccagag gttctaaaaa ccaaattaga cagctggcag gtatgcgtgg
     7321 tcttatggca aatgcatctg gtaaaacagt tgagatacca gttaaatcta atttccgtga
     7381 aggtttatca gtactagaat actttacatc ttcacatggt gccagaaaag gtcttgccga
     7441 tacagctata cgtacagctg aatctggata tttaacaaga agacttgttg atgtaagtca
     7501 agatgttatt gtaagggaaa tagattgtgg aacagaagat actacagaga tttatgctat
     7561 aaaagaagga aatgaagtta tagaagagat atatgataga attgtaggaa gatatactat
     7621 agaccctata ttaaatcctg aaactggaga agttatagtt gaggctgatt caatgataca
     7681 agaagatgaa gcagaaacta tagtagcttt aggaattgaa aaaattagga taagaacagt
     7741 tcttaactgt aaaactaatc atggagtttg ttctaagtgt tatggtagaa acttggcaac
     7801 aggaaaagaa gttaatatag gtgaagcagt tggtataata gcagctcaat ctatcggtga
     7861 gccgggtact cagcttacaa tgcgtacatt ccatactgga ggagttgcag gagctgatat
     7921 aactcaaggt ctaccaaggg ttgaagaatt atttgaagct agaaaaccaa aaggattagc
     7981 tgtaataact gaagtatctg gtagagttga aattgatgaa acaggaaaga gaaaagaagt
     8041 aaatgtaata ccagaagaag gcgagactca aacatatgta attccatatg gttctagatt
     8101 aaaagttaag caaggtcaaa tgctagaggc tggagaccct ctaacacaag gatttataaa
     8161 tcctcatgat atagtaagag taaatggtgt taagggagtt caagaatata tagttaaaga
     8221 agttcaaaga gtgtatagac ttcaaggggt tgatgttaat gataagcata tagaagttat
     8281 agtaagacaa atgctatcta aagtaaaagt tgaagaccca ggagatacag atttattgcc
     8341 aggtggatat gaagatgtat taacattcaa tgaatgtaat aaagatgcta tagataaagg
     8401 tctaagacca gcagttgcta aaagagtttt acttggtata actaaagcat ctcttgcaac
     8461 tgattcattc ttatcagcag cttctttcca agaaacaaca agagtattaa cagaggcagc
     8521 aataaaaggt aaagaagacc acttaatagg acttaaagaa aatgttatat taggtaagtt
     8581 aataccagca ggaacaggaa tgaagaaata tagaaatata gctgttgaaa aaattgaaga
     8641 ttaatagcat atagagtaaa gaaatcttga caaccattat ttgtggtgtt aaaatagtaa
     8701 agtgtgttgt ttaaaagtac actaagggct tgaacaagtt cttagtgact tttagatggc
     8761 aataaatgag aactaaaaag catcaagcta gaaaagtgct aatatcacat atttatgtgt
     8821 tatagacatt aaattgtaag taacgaatgt tacaatcagt cagtatgaat attcatattg
     8881 atgtagttgg cggtcactga taagatcgaa aatttggaag gaggtgcaga tgatgccaac
     8941 aattaaccaa ttagttcgta aaagtagaaa agcgctagag aagaaatcta ctgctccagc
     9001 attacaaaaa ggttacaact ctttaaataa gaaagtaact gacgctagtg ccccacaaaa
     9061 aagaggggtt tgtacttcag taaaaacagt tactcctaag aagcctaact cagctttaag
     9121 aaaagttgcc agagttagat taactaatgg tatagaagtt tctgcttata taccaggaga
     9181 aggacacaac ttacaagagc atagtgttgt tcttataaga ggaggaagag taaaagacct
     9241 tccaggggtt agataccata tactaagagg tactctagat acagcaggtg ttgataagag
     9301 aagacaatca agatctaaat acggtgcgaa gagacctaaa gaagctaaaa agtaaaaaca
     9361 attaatgatt gattatagtt cggtgcttta aatatatata tatttacggc atcacggcat
     9421 taaatgattt atgtcgagta cctatgattt aagttaaata attaaggagg gaagcgaaat
     9481 gccaagaaaa ggtaatattc caaaaagaga agttttacca gatcctatgt atgggagtaa
     9541 ggtagttact aaattaataa ataatctaat ggtagacgga aaaaaaggaa agtctcaaag
     9601 aatagtttat gatgctttcg caattgttgc tgaaaagaca ggagaagaag ctttagaagt
     9661 atttaataaa gcaatggata atataatgcc tgttttagaa gtaaaagcaa gaagagttgg
     9721 tggggcaaac taccaagtgc caatcgaagt tagacctgaa agaagacaaa cattaggttt
     9781 aagatggcta gtaaaatata ctagagctcg tggagaaaaa ggaatggttg aaaagctagc
     9841 taaagagata atggacgcag ctaacaacac tggagcttca gttaagaaaa aagaagatac
     9901 tcataaaatg gcagaagcta ataaagcatt tgctcattac agatggtagg atatatagat
     9961 atcttataaa attgaggata tcataaggag gataaccatg gctagaaagt ttcctttgga
    10021 aagaactagg aatataggaa tcatggctca tatagatgca ggaaagacta ctactacaga
    10081 aagaatcctg ttttatactg ggcaaactca taaaatagga gaaactcatg aaggagcttc
    10141 tcaaatggac tggatggagc aagaaaagga aagaggtata acaataactt ctgccgctac
    10201 tactgcttca tggaaagacc atagaataaa tataatagat actcctggac acgtggactt
    10261 cacagttgaa gttgaaagat ctctaagagt tcttgacggt tcagttgctg tattctgtgc
    10321 aaaaggtggg gttgagcctc aatcagaaaa tgtatggaga caagctgaaa cttatggagt
    10381 acctagaata gcttttgtaa ataaaatgga tatattaggt gcagatttct acaatgttgt
    10441 aagcatgatg aaatctagat taaattctaa tgcagttcct atgcaattac caataggtaa
    10501 agaagattca tttataggta taatagactt attaaaaatg gatgctgtta tatacaaaga
    10561 cgacctaggg gttgaaatgg aagaaacaga tataccagaa gatatgaaag aattagctgc
    10621 tgagtggaga gaaaaattag ttgaatcagt agctgaaact gatgaagaat taatgatgaa
    10681 ataccttgaa ggtgaagaac ttactataga tgaattaaaa gttgctataa gaaaagctac
    10741 tatagcttgt gaaatgaacc cagttttctg tggtacagct tatagaaaca aaggtgttca
    10801 gttagtaata gatgcagttt tagattactt accagcgcca actgatatac cagctataaa
    10861 aggtatatta gcagatggag aagaagctga aagacattct tctgatgaag agccattctc
    10921 tgcattagca ttcaaaataa tgactgaccc attcgttgga aaactagcgt tcttcagagt
    10981 ttattctgga acattagaat caggttctta tgttttaaat gcaactaaga ataagagaga
    11041 aagaattggt cgtatattac aaatgcatgc taatacaaga gaagaaataa ctaaagtgta
    11101 tgctggagat atagcagcag cagtaggatt aaaagatact actactggag acacattatg
    11161 tgaccctgct aatccaataa tacttgaatc tatggaattc cctgagccag ttatatctgt
    11221 tgctatagaa ccaagttcta aagcagctca agaaaaaatg ggtatagctc ttcaaaaatt
    11281 agctgaagaa gacccaactt tcacagttaa gactgaccaa gaaacaggac aaactataat
    11341 atctggtatg ggtgaattac acttagagat aatagtagat agattattaa gagaatttaa
    11401 agtagaagct aaagttggtg caccacaagt tgcatataga gagactataa ctcaaccagt
    11461 tgatgttgaa tacaagtact ctaagcaatc aggtggtaga ggacaatacg gtcacgttaa
    11521 gattagagtt gctcctcaag agccaggtga aggatacaaa ttcacaaaca aaactgttgg
    11581 tggatcagta ccaaaagaat atgttggtcc agttgacatg ggtatacaag gagctatgca
    11641 atctggtata gttgctggat atccagttgt agacgttgct gttgagttat atgatggttc
    11701 ttaccatgag gttgactctt ctgagatggc gtttaagatg gctggttcaa tggcattcaa
    11761 agatgctatg aagaaaggta atgcagttct tttagagcct tacttcaaag tagaagttgt
    11821 tactccagaa gattacatgg gagatgttat gggtgactta aactcaagaa gaggattaat
    11881 ccaaggtatg gaagctagaa gtggtgcaca agttataaat gcattcgttc cactttcaga
    11941 aatgtttgga tactcaacag acctaagatc ttcaactcaa ggtcgtgcaa cttacacaat
    12001 gatattcgat cactatgagc aagttccagc ttcagttgct aagaaaatag ctgaaggaaa
    12061 ataatttaaa aataattaat aatataaata taaaaaataa tcagctagat gagatatatc
    12121 tcatct
//
LOCUS       DAEORZ010000122        12125 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEORZ010000122 DAEORZ010000000
VERSION     DAEORZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.123"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            27..941
                     /gene="murQ"
                     /locus_tag="KRH39_002924"
     CDS             27..941
                     /gene="murQ"
                     /locus_tag="KRH39_002924"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBG8512811.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            1051..1971
                     /locus_tag="KRH39_002925"
     CDS             1051..1971
                     /locus_tag="KRH39_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBG8512812.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIKGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            1988..2839
                     /locus_tag="KRH39_002926"
     CDS             1988..2839
                     /locus_tag="KRH39_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8512813.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            3450..4511
                     /locus_tag="KRH39_002927"
     CDS             3450..4511
                     /locus_tag="KRH39_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG8512814.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVANFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(4627..5601)
                     /locus_tag="KRH39_002928"
     CDS             complement(4627..5601)
                     /locus_tag="KRH39_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512815.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     ISMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            6057..6938
                     /locus_tag="KRH39_002929"
     CDS             6057..6938
                     /locus_tag="KRH39_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBG8512816.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            7320..8123
                     /locus_tag="KRH39_002930"
     CDS             7320..8123
                     /locus_tag="KRH39_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512817.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            8135..9268
                     /locus_tag="KRH39_002931"
     CDS             8135..9268
                     /locus_tag="KRH39_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8512818.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINNRFIEFLKM
                     YPVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            9272..10681
                     /locus_tag="KRH39_002932"
     CDS             9272..10681
                     /locus_tag="KRH39_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG8512819.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            10892..11401
                     /locus_tag="KRH39_002933"
     CDS             10892..11401
                     /locus_tag="KRH39_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG8512820.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         4883 a         1227 c         2145 g         3870 t
ORIGIN      
        1 taatattaag aaaatgagga aatgatatgt taataaaaac tttagaaaat ttagttacag
       61 aaggtagaaa taagaataca ttgcaaatag ataaagaaga tacattaggc ataatagaat
      121 taataaataa cgaagataaa actgtagcct atgctgttga agaacaaaag gagagtatag
      181 caaaagctgt taatataata gtagatagaa tgaaacaggg aggtagatta ttctatatag
      241 gagcaggtac cagtgggagg ataggaatac tagatgccac agaatgtcct ccaacttacg
      301 gagtggattt tgagcttgtt caagctataa tagctggtgg aaatcaagca atatttaaag
      361 caatagaagg tgctgaagat gataaagaac ttggaaagca agatataata gatagaggag
      421 ttacatctaa ggatgttata tgtggtatag ctgcatcagg aaggactcct tatgttatag
      481 gtgcaatgga gtatgctaag gaattggggt gtgcagtact atcaataact atgaatccga
      541 atagtgaaat gagtaaaaaa gcggatttac caataaacat tatagtaggg gcagaagtaa
      601 taatgggttc aactagaatg aaaagtggta cagctcaaaa aatggtatgt aatatgttaa
      661 caacagcttc tatggttaag atgggaaaag tatactcaaa cttgatggta gatgttaaga
      721 catcaaatga aaagttggtt gaaagagcaa aaagaataat aatgatagct acaaatgtaa
      781 aatatgatgt tgcagaaaaa ttcttagaag aagcagataa tagcgttaaa ttggctatat
      841 ttatgataaa aagtggttta gataaggata gtgcaaagag tatcctagat agacaagaag
      901 gttatataag tgaagcttta aaatcaatag aaaaattgta aaataaataa tagtactgat
      961 aacctttcaa tattaaaaaa acagcattgg aaggttattt ttagatatta agtattgaaa
     1021 gaattattaa agatattgta ggaggatagt atgtattttt taggtgtaga tggtggagga
     1081 acgaaaacaa catttacatt agtagatgaa gaattaaata tagtaggaac cataaccaaa
     1141 ggaacttgtc attataatca aataggattt gataatctaa cgaaattatt aataactggg
     1201 ttggaagaag tttgtaaaga tgctaagata aatgtagaag aaataacgta tgcatttgtt
     1261 ggacttgctg gatatggaaa aataaaagaa gtattgtatg cattagaagt agcaacaaaa
     1321 aatgcatata gtcatataaa ttatactcta ggtaatgatg tagaaatagc attagcaggt
     1381 tctttaaatg gggaaaaagg tataaatata attgcaggta ctggttcaat agcacaggct
     1441 ttagataaag atgggaatct tcacagatgt ggaggatggg gctatgtatt aggagatgaa
     1501 ggttcagcgt attatatagg aatggctacc ttaaaaatgt tcacaatgca gtctgatgga
     1561 aggtgttcaa aaacaaaatt atatgattta ataaaaagac atcttaatat agagaatgat
     1621 tatgacataa taaaatatgt aaatgatgaa ataaaaggag atagaataga aatagcaaag
     1681 tttgctacga tatgtttaaa agcagttatt gaaggtgata atactgctag taaaatattt
     1741 gatgatgcag cttatgaatt aagtaggctt ataataggat tagagcatca ttttgaacaa
     1801 ggtacaaaga taaaggtatc ttattcaggt ggagtgttta agtcaggaga tttaatatta
     1861 gagccactta aaaaatattt gaataaaacg agatttggta tagtgagacc agtgttgact
     1921 ccagatttag gagcatgttt acttgctaag aagaaatatt atagtaaata ggtagaggtg
     1981 atagagtatg agttgcattt taaaattaaa acagatatat gaagatttaa cggaagtaga
     2041 taaaaaaata gctgattaca tacttaataa tacagaggct ataagtaagc tttctgtatc
     2101 agaattggcg tcaaactcaa aaacaagtac agcaagtata gttaggtttt ctagaaaaat
     2161 gggatattcg ggttttgggg atttaaaaat tgagatagct aaagatttga tgggcaaaga
     2221 aaatgaatac acatatgtag atgaaaacta tgattgtaat atagatagtg ttataaacaa
     2281 aattacaaac aaaaacatag agactatcaa ccaaacaagg ttgttgaatg aaaaagatat
     2341 aataaaagaa gcagttgaac agataataaa ggctaaaaat gtatatatat ttggagtagg
     2401 tggttcggct ttagttgcac tagatttaca gatgaaactt ttaaggataa ataaacaggc
     2461 atttacctct ttagatagtc atacacaact tatggtatct tcaaatgtag ataaagaaga
     2521 tatagctata gcaatatctt actctggaga atcaaaagaa gtaataaagt ctatagaaaa
     2581 tgcaaaatta aaaggatgta aggtcatttg tataactaaa tatagtgaca atcatcttag
     2641 taagattagt gatttaaagc tagtagtacc aaatatagaa aaaaggctta gagaaggagc
     2701 tatttcttca agaatagcaa tgcttacgtt gatagatata atatatattt ctataattca
     2761 agaaaactta aatgaggcag aacagaaact acaagaaaca aaaaggatac ttgattattt
     2821 aaatattaat aatgcttaaa actaggttat atatcaacta aacaaataag ttagactata
     2881 agcgtaagtc aataagaaat agataaatat ctaaaatatt gacttactta ataataatat
     2941 atagcatttt tataatacaa atgagttgtc ttaaaattga ttttttttaa tcaaatttta
     3001 agttcaactc cttttttata tttttataga tatatctata ttttcccaga aaacgtattg
     3061 aatatacaaa ttataagaag tataatgttg ttgggagaga atgtatgaaa tgtatacaaa
     3121 gttgatttta actgtattat tgataattct ctttactctt accattatta atgattgaag
     3181 acaatggtat ttattatagg tcaaagattc cgaatgtatt aatcatcatc attttctcaa
     3241 actaacagca aacaccctga tttgcgagtt gtattgtata caaattatct agaaaatatc
     3301 tattagattt tccatgaaat ttaaatattt cacgaatgta tgaatggaga aatatacatg
     3361 ttgacaatcc acctttttaa atcctaaatt aattttatta atggtagaga ataggaagta
     3421 acaatgtttt ataaaggaga atattaagta tgaagaaaat taggaagtta gctaccaagt
     3481 tattgattac aacaatagtt ttaatctcag gtatgagtat gacagtttat ggtatgacag
     3541 ccaaagaggt aacagcaaaa acaccaaaaa gctatgtcac tggaaccaat agcgtatatg
     3601 gtccaaagct gtcacaagcc caactaaact cagtagcaca agcaacagca gattttatga
     3661 acaagaaaat cacaaagaat atgaccacag atgctaagat tttagttgcc tacaatcaca
     3721 ttaaaaataa cacaacatat gttgactgga atgcagtaga gggagcaaat actgcctata
     3781 cattggttac taaaaaaggt gcatgctcag gaatggctag gagcatgaaa gctttgtgtg
     3841 atgctatggg aattgaatct tattatgtac acagcacatc aaatgaccac cagtggaatc
     3901 ttatccgttt tggtgatggg cgtttgtatc atgtagatat tgatgctaat aaatcagcag
     3961 gaaaggatat catatacaag agtcttagcc acccattacc atttgataag acagcatatc
     4021 caaaagttgg tgcaatagta cagacggtaa aaccagaaca gacacagaaa ccagcaccac
     4081 ctgggaagca cactatgtta ccacatcaag aaggggcaaa ggctgttgca aattttggaa
     4141 aaaaaggaga agatataaat gaaaaacatg taatgtatga aggtgttaga tgtagtgtag
     4201 ttaattcatc agtaaataca attacattta caggtagtga atcaaattta gacccatcaa
     4261 atcttacgat tgctgtaata ggatttatga acacaccata tgggatgttt gcattagcac
     4321 cagagggtaa acctgttgaa tatcctttta aatatgataa gccattttct ttagatttta
     4381 gtggtaaaaa tcgttctggt gcagaattag cgaagtactg taaaaaaaat gatgtgacta
     4441 ttaaaataga aatcactgat gaaaaagcta atattggact tggtcaaata atgtatgttc
     4501 gatatgaata atgatttaat catgacagta gaataagtta taaacattgc gccctcttac
     4561 taggggcgca tttaatatta tatctgatat tttgtagtca ggtcaactgc tagatttctc
     4621 ccctttttat ttataaaaat tattatctct aagcatctga gcatgtttac gattaactaa
     4681 aaactgtttt aactcagtca agcataggaa taaaatagct ctattttcaa actctgttct
     4741 gtcaacaggc agaggctggt ttttcatact aatcattagt ctctttaatt cttcaagaag
     4801 aagattgcct gtttcagctt caaattcagt ataacctatc ttatgtataa aagcagatag
     4861 aatatgagct tgtggtggtg tagaatttag ttgagataca taactataca aattttctaa
     4921 gatatcccgt tggcttttta taatatccat atgttctaag aagaatctag tatcacttaa
     4981 taaagtatta ttaatattat cgtaagtttc agaaatactg ctgtcaataa gtgaatttaa
     5041 tgtatataaa tctctactat atccattttc ttttttagga tttacaataa tatctgcaat
     5101 gtctataagt acaatactca cttcttcctg caactttttt tgaacttcat ggattttatg
     5161 tatatttgtt ggaatataca tatttattaa gacacctatt ccagttccta ctgcaaatat
     5221 tccagcttca tttaaaatcc atttgataga agtttctcct tgaaggtagt aatgggatgc
     5281 tatgactaca cacatagcaa taacatcttg aatatttaac aaaaaacaaa gtggtataat
     5341 tataaatata aatattgaaa atgaagtcaa attatatcct aaaatattaa aacataaata
     5401 tgaaaaaata gtacataaaa gaaaaccata aatctttcca atggagcctt taagagtttc
     5461 tcttttagtg tcctttactg taagtagtac tataactcca gcaaccattg agtattctaa
     5521 ttttaatagc caagcaataa atatagcaag agaacatcca agagataatt ttattacttt
     5581 aagtgaatta atattattca aatgcaaacc tcctgtaaat caatataata aaattctaac
     5641 ataaaactaa tattgcttga aaggtaagta actatactta attattttat tgccctattt
     5701 gtttagatac aatcaatata attttatttt atgaaaattt tttgtaaaat tttaaattta
     5761 gagactaaca tgagaccaca aagaggaagt ttattatagg aagaaaactt aaaattgatt
     5821 ttaagcagca aatgaaaata atcaaatatg tatatactat tattaaaaaa taaaattata
     5881 tagttaatat aattttataa ttggtttagt atagagattt ttaactttta tgttatacta
     5941 tttataaaag taggtataaa gattaaaata cataatcaaa tatgtataag aggttttaaa
     6001 tagtattgtt aatgaacttg attattgttt ataattacaa attggaggag ataaacatga
     6061 aaattactga aggatatatg ccttttaaag gttttaaaac gtattaccgt atagtaggag
     6121 aaaatacgga agggaagaag ccattagtat tacttcatgg aggtccaggt tctacacaca
     6181 actattttga ggtattggat aagatagctg aaagtggtag acaagtaata atgtatgacc
     6241 aaattggttg tggaaattca tttgtagaag ggcatcctga gctatttaat gcagatactt
     6301 ggatagagga gcttattgaa ctaagaaaac atctaggact tgatgaaatt catctgttag
     6361 gtcaatcttg gggaggtatg caggctattt ggtatgctat tgaatataaa ccaaaaggta
     6421 ttaagtctta catcctttca tctactcttt cttctgcaaa actttgggag aaagaacaaa
     6481 aaagaagaat atcatatatg agtgaggttg accaaaaagc attacttgat gcagtaaata
     6541 ctggagatta ctctagcaaa gaatataatg atgcattaga aagatttatg gaaatgtatt
     6601 gtgcaggtga agttacagaa gattctccag agtgcctgag aagacctaaa aaatcaggtt
     6661 ctgaagcata tattgtagga tggggacaaa atgaattttc tcctacagga actctttctg
     6721 gatatgaatt tacagataga ttacatgaaa taaaagagcc ttgtctagta actagtggag
     6781 caatagattt atgttcacca tatattgcaa agactatgta tgatagaata cctaatagta
     6841 agtgggaatt atttgagtat tcaagacata tgccattcgt tgaagaaaat gagaaatata
     6901 taaaagtctt aactgagtgg ttgaatgcga acgattaaaa actatataaa tttatataaa
     6961 aaaatgagtg tctcaaaatg aactttttag ttcattaggc actctttttg catgaaatca
     7021 aatatatttt cattatttga acgataaaat gaggcttata tctattttgg gaaagtaatt
     7081 ttttattact agtgttagat tctaatctaa ttgtatactt attgaaataa tagaaatata
     7141 ttggtgttta taacttaatt ttattgtttt ttagaaattt ttttatatat tggtataatt
     7201 attctgttta aatttgatta aaaagtatat taattataat aagaaacaat tatataaata
     7261 ggaatagatt attataatta atatagatat aaaaataatt atagaaggaa aggcacatta
     7321 tggaaaaatt gagagttatt gaaatgataa aaaataatcc caatattata gctacaatag
     7381 ataatccaac tgatgaaatg aaattgttag ctattaaaga aaatgggctt gtactagaat
     7441 atataaataa tccaaccaga gagatgcaag aactagcaat agataataat attagagcaa
     7501 ttaaatttat agataatcca acagaagata tgatgataaa agcagtaaat gagggatgga
     7561 gtatactgga ttatatcaaa aacccaactg ataaagttat tgaaatggct ataaatcaag
     7621 caggatgggc aattaaatat gctaaaaatc caagtgaaga attacaactg ttagcagtaa
     7681 gaaaaaatta tgattcgata aagtttataa aacaacctta tgaaagtgta caagaggagg
     7741 cagtgagaat aagttatgat gccttgagat atatagattc acctagttat aatgcagaac
     7801 ttatagctat aaaaaataat gaagcagcta ttagctttat aactgattta gataaaaata
     7861 aaatgttgga atttttaaaa gtgaatattt tagtgattaa gtacattctg aaaaaagtat
     7921 ctaaagatga attagagagt gtattaaaag aagtattatc caaagaagat gttgaggaaa
     7981 agtatattag agattttttg aattgtagtg ttatagatag aaatagtgaa agtataccaa
     8041 tagataaaat tatgtttatt tataaatatg gaagtaagac agcaaaaaaa atagctgtag
     8101 atgagaaatt aaagattatg taggagagag aaaaatgaat tttatagaca cattaaaaag
     8161 cgttgactta atattatcca ctggagaagt acctttaata gtaggtgaaa gtggaatagg
     8221 aaaaacagct ttagcaaata agcttgccaa agaaaatgat tggagtttaa ttgttattga
     8281 tggaaactta cttaaagagg gtgaaatagg tggtcttcca actatagaat cttatgtagg
     8341 agttaattct aatggatata agactgaaaa gaaaacaaca gtgtatgctg ttcataacaa
     8401 attaagagaa attgatgaag agatatctaa aagtaagaca gttcttttat ttatagatga
     8461 gataaatcgt tgtgagcata cagtacagca agaacttatg aatttaattt taaatagaga
     8521 aattaatgga tataaactgc atgatgatgt aaagatttta gcagcaatga atccatcaag
     8581 taaatatggt tcagattttg attatcaagt ggtagatatg gatgcagccc aagaaaatag
     8641 gtttgtttgg ttaaatatgg agagtgacca tacacaatgg ataaaatggg caatagatga
     8701 gggtattgaa agaaaagtta ttgagtttat ttccactttt ccagaatatc tgcataaaat
     8761 aaatgaagat gatgtaagag ctactcctag aagttatgag agagtatcta aaatttataa
     8821 agtttataaa gaaaaaaata attctattcc tagagcagta tttttaaatg ttgtaaaggg
     8881 taatgtaggt aaagttattg cagaagaatt tataagtttt attgaatcaa actctgaacc
     8941 attaatatcc tatgaagatg tttttttagg agaatctatt gatgagtcta ttgtagaaag
     9001 agtaaaaaat gagagtcata caagacttta tctatcagct atgaacattt taaaagactt
     9061 agaattaaat attaagaatg ataaatatga atcaaaccac tatattaata atagatttat
     9121 tgaattttta aaaatgtatc ctgtagattt aatgataggt attatgaagg atattagaaa
     9181 cagttatatt gaagtatata aaaaagctat agaaaatgaa gagtttgtaa aatcatattt
     9241 tgaatcttat agtttaataa ggggataatg tatggaaaat tattttgata aacaagcaaa
     9301 agagctttat aataaagcaa gtgaaattat aaatactcat tctatgctaa aggcaaatcg
     9361 tgctaatgag aaatttgata tagatatacc acaagatttt aagtatgagt tttttagtct
     9421 tgtagataaa gtgaatttaa gtcttatgga agaagaagat aatttttatg gatatttttt
     9481 gtttcagatg tcaagagaaa taagatttga tataagtagt cctacaggtg tgaattttaa
     9541 gggagctaaa tatgttatat attttaatcc aataatcttt ttaacgcttg acataaagca
     9601 aatggagact acaattaaac atgaaattca tcatatatta tctatgcatt tgatgagagc
     9661 aaaggagtta aaaggaaagt atagtacatt agcaattaat atggcaatgg atatagtagt
     9721 aaataaattt ctaaacaatt taccaccata tgcaactacc ttagagtggg taaatttaaa
     9781 gtattcctta aaacttgaac catatgaacc ttttgaatat tatgtagaaa agattcagac
     9841 tgaattagat ttgatggaag tggatgaaga tggtgaggaa gatgatagtg ataaatatgg
     9901 agatatagaa attgattata gccccgaaag aactcatgat atatgggatg aatccaatga
     9961 gatagatgaa aataccctta gagaatttac tgaaaaattt attaacagtt ctcaaaaagg
    10021 taaagttcct gcttatttag aaagtatgat atcatcactt aaaaatagta agggtgaatt
    10081 gccatggaat ttatatctta atagaataat gggaacagtt gaaaataata aaaagaagac
    10141 tataacaaga agaaatagaa gacaaccaaa tcgtttagat ttgaggggag aacttagaag
    10201 tcataaggca gaaatagcag ttgcacttga tgtaagtgga agtattagtg atgaagagtt
    10261 taaacaagct attaaagaag ttcttagtat agtgaaaaac tataatcatg aaattacaat
    10321 tatagaatgt gataatgaaa ttagacgtgt atataaagtg agaactgaaa aagatataaa
    10381 agatagaatt aggataggtg gaggtactag attttctcca gtctttgagt atgctaataa
    10441 taaaaagatt aatttgttag tctactttac tgatggtaaa ggtgaaaata aactacaagt
    10501 aatacctaga gggtataaaa ttttatgggt tatttctgga agaggagata aaatttcatt
    10561 aaaagaacct tatggagcag ttaaaaaact tactaaggtt aaaacaaatg atgaaatatt
    10621 agaaataaat gatgtaagaa atgatgggta ctcaatgaat agccaagcac caattttata
    10681 ggtattcaca ggagataagc tgtattaatt tattttgata tgcttgttat aaaaatctaa
    10741 aaaatacatt aatcaatggt taatgtattt tttaatttta ataatataag ttttacttac
    10801 atttaagtct caaaatgtgt taaaatataa tatatagcag atagttttgg caaggtggat
    10861 ttagttgtca aaactatttt ttttattaag gatggtgatg tttttgttta agattgatga
    10921 ttatataatg tatggtatga caggagtctg caaagtcatg gatataacaa atgaacgatt
    10981 tacaaatgga ataaaaaaag agtattatgt attgagtcct atttattcta ataatacagt
    11041 aataaaaatt cctgtagata atgaaaaagt accaatgaga aagttacttt ctaaaattaa
    11101 tgtgctttca ttaataaatg atattcctaa tatggataca tcatggatag ataatgaaaa
    11161 attgagaagt gagcaattta aaaaaatact cagaggtggt aaatgtgaag agttaataaa
    11221 attagtaaga agtatagata ataacagaga gtatgtaaaa tctattggta aaaaaacaca
    11281 tcaagcagat gataatataa tgaaagaagc tgaaaggtta ttaaatgaag aatttgcaac
    11341 tattttagat atttctccta atgaagttag ttcatatatt tcaagtcata tacctcaata
    11401 aagtgtttta gtatccttta tttaatctat tttaagtatt atttatgtat gctggttttg
    11461 gtaaaatgaa atatataatt aaattatata aaaaattaag aatgattgta ttgaagaatt
    11521 acagaatagt gcggagagcc tactccttta ggtgtgagat ggatatcact taaagattta
    11581 aattgttatt gtagatgttt ttaatataaa acaagattaa taagtgtatt gtaagcttta
    11641 agtgaaattg atactataat gagttactta gattttaaaa tatataatat tataaaaaat
    11701 ccttttaggt tttgaatgaa taagatggat actatgttga atagaatcca tcttatttaa
    11761 gttttattta tgttgtttat ttgtaagcat tatttttgta catgaaaaca cagtgtaaaa
    11821 atatgcttag aattatgaag ttttattaaa aagatattcg acaaaataag actaaaaata
    11881 ttcttaaact tataactaag ataaatattg tatatttata gtgtagcctg aaaaattgtg
    11941 gatgtacgaa atttatttag tattttattg aaacaaagta gaagaagtac tatagtttat
    12001 ctttgaatag attgttattt tttagtgaaa aagttattag gttaatacat aaatataata
    12061 gcttataaat agtttaagca tgttaaaaaa gtagttatta gagatatata gttacattgc
    12121 ttata
//
LOCUS       DAEORZ010000123        12110 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEORZ010000123 DAEORZ010000000
VERSION     DAEORZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.124"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..216
                     /locus_tag="KRH39_002934"
     CDS             <1..216
                     /locus_tag="KRH39_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG8512821.1"
                     /translation="GLKEAGIDSEITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHT
                     IDEYIELDQLFKGAEGYYGILKSVYKK"
     gene            232..1815
                     /locus_tag="KRH39_002935"
     CDS             232..1815
                     /locus_tag="KRH39_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG8512822.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIRFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1843..3171
                     /gene="ssnA"
                     /locus_tag="KRH39_002936"
     CDS             1843..3171
                     /gene="ssnA"
                     /locus_tag="KRH39_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBG8512823.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3370..4722
                     /locus_tag="KRH39_002937"
     CDS             3370..4722
                     /locus_tag="KRH39_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG8512824.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            4914..6023
                     /locus_tag="KRH39_002938"
     CDS             4914..6023
                     /locus_tag="KRH39_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8512825.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6306..7697
                     /gene="hydA"
                     /locus_tag="KRH39_002939"
     CDS             6306..7697
                     /gene="hydA"
                     /locus_tag="KRH39_002939"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG8512826.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDSVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7691..10252
                     /gene="xdh"
                     /locus_tag="KRH39_002940"
     CDS             7691..10252
                     /gene="xdh"
                     /locus_tag="KRH39_002940"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBG8512827.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRSKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYENHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10404..11750
                     /gene="rpoN"
                     /locus_tag="KRH39_002941"
     CDS             10404..11750
                     /gene="rpoN"
                     /locus_tag="KRH39_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBG8512828.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT         4820 a         1406 c         2462 g         3422 t
ORIGIN      
        1 ggattaaaag aagctggaat agattcagaa ataactcaat actcattctg tacgaatgga
       61 agtcactacg caggtgaggc tggaataaga actataggat ttggtccttc taaggaaaat
      121 cttgctcata caatagatga gtatatagaa ttagaccaat tattcaaagg tgcagaagga
      181 tattatggta tattaaaatc agtttataaa aaataattag gagatggttt tgtgagaact
      241 ttaataaaac atgggcttat agtagatgga aataaaacac cagcatatga aggagatatt
      301 ttaatagaaa acgagaaaat attaaaaata tcacaagatt taaacgaaga agctgacaaa
      361 gttatagatg caaaaggtag agttatatgt ccaggtttca tagatactca tagccattca
      421 gacctagtta ttttagttaa tccatataat gaggtaaaga ttagacaggg aattacaaca
      481 gaagtgttgg gccaagatgg tatatctatg gcccctctac ctcaagagca tatatcttct
      541 tggagaaaaa atttagcagg tttagatggt gaaagtgatg aaattgattg gaaatatgaa
      601 acaacagaga attatttaaa aatgatggat tacaatgggg taggtttaaa tgaaacttat
      661 ttagtacctc atggaaacgt tagaatggaa gctatgggac ttgaagatag acctgctact
      721 aaagaagaaa ttcaaaaaat gtgtgaaatc acagagagag aattaaaagc aggagctata
      781 ggattgtcta ctggacttat atatatacct tgtgcatact cattgactga agaaattata
      841 gagatgtgta aggtagtagc aaaatatgac ggagtatttg tagttcacca aagaagtgaa
      901 gcagatacaa ttttaacttc aatggaagaa atcatagaaa ttggaaaaca atcaggagta
      961 aaagttcatt tttcacattt taaggtttgt ggtaaagcaa actggaagta tataccacaa
     1021 gttatagagc ttttagaaaa agcagaaaag gaaggaataa gagtatcttt tgaccaatat
     1081 ccatatgcag caggaagtac aatgcttgga gtagttttac ctccttgggc acattctgga
     1141 ggaactgata agttaataga aagattaagt gatgaaaacg aaagagcaaa aatgaaaaaa
     1201 gatatagcaa atggaataga aggctgggat aattttatag aatttgctgg aatagaccaa
     1261 atatttgtaa caagcgtaaa aacagaaaag aataaagata caattggtaa gagcttatta
     1321 gaaattggaa aaatgagagg aaaagaccca ttagatgcaa cgtttgattt attaaaagaa
     1381 gaagaaaatg cagttgggat ggtagatttc tatggattag aagaacatat aataagattc
     1441 atgaaaagag acgagcaaaa tgtgtgtaca gatggtcttt tagcaggtaa accacatcca
     1501 agagcttatg gttctttccc taaaatacta ggaagatatg ttagagagtt gaatgtgtta
     1561 acaatcgaag aagctgttta taaaatgact aaaaaggcag ctacaagctt tagtataaaa
     1621 gatagaggcg aattaaaaga aggatatttt gcagatatag ttatatttga caaggataca
     1681 gttagtggtt gtgatgattt tatcaactct atgcagtacc ctacaggtat agattatgta
     1741 attataaatg gaaattgtgt tgttgaagaa ggaaaataca ataaaattaa agcaggaaaa
     1801 gtacttaaaa attaaatctt agctttaagg aggaataaat ttatgatttt agtggggaat
     1861 gggaggctaa taagccaaga ttctttaaat ccctatatgg aagatggatg tgtagtaata
     1921 aatgataatc taattgaaga tataggtact acagaagaaa tgaaagctaa atatccagta
     1981 catgaattta tagatgcagg caagaaaata atcatgccag gtctaataaa ttcacatatg
     2041 catatataca gctcatttgc tagaggaatg gcagttccag gaaaaccttc tgagaacttt
     2101 ttagagatac taaacaattt atggtggaga cttgacaaac agcttacact ggaagataca
     2161 aaatacagtg catatgcaac gtatatagaa tgtataaaaa atggtgtaac aacagtttat
     2221 gaccatcatg caagtccaaa tgcaattgaa ggcagtttgt ttacaatatc agatgttgca
     2281 aaggatttgg gaattagaac gtgtctttgt tatgaagtgt ctgatagaga tggtggaaat
     2341 acaatccaac aaggtatcaa tgaaaacgta aactttataa aatacgctca aaaagatgat
     2401 acagacatga taaaaggaat gtttgggctt catgctgcat ttacactatc tgatgataca
     2461 ttaaataaat gtgcttcaga gatggctggt cttgatacag gttaccatgt acatgttgct
     2521 gaaggactag cagacttaca atataatgta gataaatatg gaaaaagggt tttagaaaga
     2581 ttcaatgatt ttggagtact aaaagaaaaa acaatagcag ttcattgtat atatataagt
     2641 gcagaagaaa tcagtctttt aaataaaaca aatacatttg tagttcataa tccagaatct
     2701 aatatgggaa atgcagttgg atgttctcct gcaatagagt tattaaaaag gggagttaca
     2761 gtaggattag gaactgatgg atatactagc gatatgtttg aatcattaaa agttgccaat
     2821 ataattcaca aacatcactt aaaagaccca agagttggat ttgaagaatc acaaacaatg
     2881 ttatttgata acaatagaaa aatagctaaa gcttattata aaaatgacct aggagtactt
     2941 aaaaaaggtg cttatgcaga tgttatagta gttgattata taccacatac tccattgaca
     3001 gaaaatacaa tagggggaca cactattttt ggaatgagtg gaagaagcgt agatacaact
     3061 attataaatg gaaaggtact tatgaaagat agaaagttaa taaacataga tgaagaaagt
     3121 attctagcaa aaagtagaga attaagtcaa aatctatgga atagaattta aataattgat
     3181 tatgcaaaat tctatgaagc aggaaccctg tgactttagt catgggaggt tcagattaat
     3241 atacaagtaa gaaacaagaa tttagatgtt aaaaacagag cttaaacaaa aataggaatt
     3301 taaatgaagt atataaataa aaaatatacg gaatagaata ttaaattaaa tatagaaggg
     3361 agacccaaaa tgtctcgaga taaaagccct aaaatagctc cagtagatga aaggttacct
     3421 gtatccaaat cttgggtatt ttcactacag cacgttttag caatgtgtgc aggagcagta
     3481 gcagttccta tgatgatagg aagtgctgca cgactagatc atgctagtat tgttttttta
     3541 ataaatgctt gcttgtttat ggcaggtata ggaacactca tacaaagtgt tggtatagga
     3601 aatattgtag gtgctaaaat accagtaata gaaggaacta gttttgccgc tgtggcaggt
     3661 atgacagcaa tagcctcatc atatagtgat tcacaagttg caatacagac agtatttggc
     3721 tctgtaatag tagctggatt gttttgtata ttacttgctc cagtatttga aaaattaata
     3781 agatttttcc ctagtgtagt tactggaaca gttgttatgg taataggtat ttcactattt
     3841 ccagttggga taaaatggat aactgacaac aaattagaag cggttgaacc aaactcatta
     3901 gcattagctt tagcagtatt ggcaataact ttactttcat ttaaattctt aaaaggaatt
     3961 tggaatagca cagctatatt ggtaggtata gtacttggta cactactttc catggcagtt
     4021 ggaatggcag attttagtgc agtaaatgga gttggttggc ttaacttaaa tgtaccttta
     4081 aaatttggaa tgcctagatt tgaaccatct gcaatactat cattgtgttt gattatgttg
     4141 gtacttatga cggaatcagt tggtaatatg atagctattc atgaaatggt agacaaggaa
     4201 gtaacaggta aaaatattaa aagaggtcta tttggagatg gaatatctac agcattatct
     4261 ggaatattca attcatttcc aataactcca tttggtcaaa atacaggaat agtaggtctt
     4321 acaggtataa aatcaagatt tatagcagtt tacgcagggg ttatattgct agtacttgga
     4381 ttcttaccaa aatttgcagc aataatagga gcaataccta agcctgttct tggaggcgta
     4441 ggatttgcaa tgtttggaat ggtaatggtt ggaggagtaa aaactttaag caaagtgaaa
     4501 tttgatggaa ctaaaaatgg ggttatagta gcagttagtg ttggtttgtc tatgatacca
     4561 ctaacaaatc ctgatttcta tcataatttc ccaacttggg tacaaacaat attccatagt
     4621 ggaataacaa caggtagttt atcagcagtg cttttaaaca tattcttcaa tgaactaggt
     4681 tctaaaaaag aaaaaaataa taaagttgaa gaagcaaaat aaaaataaaa caagtaaaaa
     4741 tctatattga cttaaagtca ttaagtatta ataaaaataa ttcagagata ttaataattc
     4801 aacaaaacta tcttatattt taaataatat aaaaaagtaa ataaataacg aatacaaata
     4861 aaagaataca atctaaatat taaaaatatt taatttaagg gagagataat aatatggcta
     4921 atttaaaagt taatttatat gggatggaat ttttaaaccc aattatgaca gcggcaggac
     4981 ctggagcaaa agatggtgct ctctgtcaag aggcagcaaa aggtggagca ggagctatat
     5041 gtacaaaaac tatatctgta ttaccagctg atgtaccaag accatgtatg gcaaaaacta
     5101 atagtggttt tttaaacaca gaattatggt cagagcttcc aaaagagcaa tggatagagg
     5161 aagaatacaa aaaggtaaaa gaaacagggc ttccagtaat agtaagtatg ggatattcag
     5221 cagagcaaat agctgaggtt gctcctttag taaagcctta tgcagatgca gttgaattat
     5281 ctactcatta tgtaggtaca gacgttgcac caatagtaaa tgctttaaaa gctgcaaaag
     5341 ctgttttaga tgtaccagta tttatgaaaa tgagtcctca tactgacatt caaactattg
     5401 caaaagctgt tgaagaagca ggtgcagatg gacttgtaat gataaactca tttggaccat
     5461 gtatggcaat agatgtaaat acaggatacc ctataatggg aagtaaagct ggatacggat
     5521 ggttatcagg tgcaccaata agacctctag caataagatg tatatatgaa gcttctcaag
     5581 ttgtaaatat accaataatc ggtgttggtg gaatcacttg tggaaaagac gtggctgaaa
     5641 tgatgatggc tggagcaagt gctgttcaag tatgtacaga agcaatcctt aaagggcctt
     5701 ctatctatgg aaaaattgct aaagaattaa atgaattttt agatgagaat ggatatgctg
     5761 atgttaatga aataaaagga ttaactcata agaaaatggc agctagaggg tttagaactc
     5821 attcagtagc tccagtagtg gatgataaca agtgtatcaa atgtaagcaa tgtgtaacaa
     5881 gctgtgtata cgaagcttta gaagttacag ataagttgaa catagatgca gacaagtgtt
     5941 ttggatgtgg attatgtgta actagatgtc caaaaggagc tttaagcatc tgctacaatc
     6001 aagtagaagc tatgaatgag taataattat atagaatttt ggtaattaac ttagagtaat
     6061 aggtattagg ataataaaat atatcagata aacaaacaca cattatgcat taataataca
     6121 gtctaaagtt aataatttta ttatattgat ttaactaaat attagtttag ctgaatatta
     6181 atttaattag gtattggttt aatcttaata tagatttact gcgtataagt gaactaacta
     6241 gattacttta agttaatttt ttcatataaa ataaattgat acattacaag taaggtggag
     6301 tctatatggg aattgtttta aaaggtggga aagtagtaac aagtgaaaaa acttactgtg
     6361 cagatgtaag aatagaagat gaaaaaatag tcgatatagg tcttgatata tcaaaagaaa
     6421 atgatgaaat agttgatgtt agtgattctt atgttatacc aggaggtata gatacacaca
     6481 cacactttga cttagataca ggcgttacta agacagcaga taactttgag acaggaacaa
     6541 aggctgctat agtaggagga actactacta tattggattt tgctacacaa gctaaaggat
     6601 gttctttgaa agacggatta gaagaatggc atgaaaaagc taaggataaa tcctattcag
     6661 attatggctt tcatatggct ataacagatt ggaatgactc ggtttgtaat gaaatggagg
     6721 atatggtgaa agagggagta tcttcattta aactgtatat ggcgtataaa ggaagccttc
     6781 aagttgatga tagtgtaatc tttgaagcat tgagaaaagc agaagagata ggagggatta
     6841 ttggtttcca ttgtgaaaat ggggacataa tatgtgaact tgtggataaa gcaaagtcgg
     6901 aaaataattt atctccaaaa tatcatcaat taactagacc agctataatg gaggcagaag
     6961 ctacatcaag acttatgaag atagctgaag ttgcaaattc taaagtatat gtagttcact
     7021 taagctgtaa agaatcctta gaggaggttt taaaagctaa gcaaagaggt gtggatgtaa
     7081 tagttgaaac ttgtcctcaa taccttctat taaatgatga attatatgat ttagatggat
     7141 ttgaaagtgc taagtatgta atgtcaccac ctttaagaaa taaagaaaat aatgaaatat
     7201 tatggaatgc tattttagat aatcaaatag acactatagg aacagaccat tgttctttta
     7261 attataaggg acagaaggaa cttgggataa acgatttcag taagatacca aatggttctc
     7321 caggtgtaga acataggatg agtcttttat acacatatgg agtctgtaaa aataaaatat
     7381 ctatcaacaa gatggtagaa gtaacatcaa ctaatgcagc aaagttgttt ggaatgtatc
     7441 ctaataaagg aactatagaa ataggcagtg atgcagatat attagtctta aatccaaaca
     7501 aaaaatccaa aatagaagca aaaaaacaat tacaaaatgt agattataca ccttatgaag
     7561 ggtatgaagt ggattgtcaa atagaacatg tttatttaag aggaaaagaa gtagttagag
     7621 atggagaatt aatttgtaat actccaagtg ggagatatat aaataggcgt ctaacaaagg
     7681 gggctagaga atggtagaat ttatattaaa tggagagaaa gttgttgcaa aagaaggcaa
     7741 aaaactttta gacttcctta gagaagatat gaatataact tcagttaaaa atggatgttc
     7801 agaaggtgct tgtggaacat gtatggttat aatagatggt aaagctacta aagcatgtgt
     7861 atttaaaact gataaattag ctgggaaaaa cataacaact atagagggat tatcagagag
     7921 agaaagagat gtttttgcat attcttttgc tagtcaagga gctgtacaat gtggattttg
     7981 tattccaggt atggtaatta gttcaaaagc acttatagac gttaataata atcctactga
     8041 agatgatata aagaaagctt taaaaggcaa tatatgtaga tgtacagggt atgtaaaaat
     8101 aattaaagct gtcgatttag cagcaaagat ttttagagaa aatatagatg tgccaaaagt
     8161 aaactgtaag ggtttagtag gtgaaaatct tcctagagtt gatgcagttg gcaaagcact
     8221 tggatattct gaatatgtag atgatatgag aattgagggt atgatttatg gttcagcagt
     8281 tagatctaag tatcctagag ctttagttaa aaaaatagat atagaagaag caaaagctta
     8341 tccaggagtt gttgcagttc taacagctaa agatattcca ggaactataa aggtagggca
     8401 cttaaagaag gattgggata ctttaatacc agaaggtgaa ataactagat acttaggaga
     8461 tggaatagtt ttagtagcag cagaaactag agaagcgtta gaagaggcca aaaagctagt
     8521 taagatagat tatgaggaat taacaccatt aagtactaca gcagatgcac ttagagaaga
     8581 tgctccaaag atacatgaga cagggaatat acttgttaag gaacatttag taagaggaaa
     8641 cgctaaagaa aagatagaaa attcaaaata tgttgtaact aaaagatata ctacacctgc
     8701 tacagaacac gcatttttag agcctgaatg tgcagtagct ggtccttatg aagatgatgg
     8761 agtaataatc tattcaacag accaaggggt gtttgccact caacatgaat gtgcagatat
     8821 gttaggatta ccatatgaga aggtaagagt tataaacaaa atggtaggag gagggtttgg
     8881 tggaaaagaa gacatgagtg ttcaacacca tgcagcctta cttgcatacc atacaaagag
     8941 atttgtaaaa gtacagttaa caagaaaaga aagtattatt gttcatccaa aacgacatgc
     9001 cacagaaatg gaatttacga cagcatgtga tgaaaatgga tacttaacgg gtatgaaggc
     9061 aacgataata tctgacacag gtgcatatgc ctctcttgga ggaccagtgc tacaaagact
     9121 gtgtactcat gcagcaggac catacaatta tcaagatatt gatataacag gaacagcagt
     9181 atatactaat aaccctcctg gaggagcatt tagaggattt ggagttacac agagttgttt
     9241 tgcaactgaa tgtaatctaa atttacttgc tgaaatggtt ggaatatctc cttgggagat
     9301 aagatataaa aatgcaataa gacctgggca agttttacca aatggtcaat ttgcagatga
     9361 aggaacagca atggcagaga cactagaagc tgtaaaagaa gagtatgaaa atcatccacg
     9421 tgcaggtata gcttgtgcta tgaaaaatag tggggttgga gttggtatac ctgatgttgg
     9481 aagatgtaga ttggtgataa aagatggcaa agttcacata agaacaagtg cttcttgtat
     9541 aggtcaaggg cttggtactg ttatgactca agtagtttgt gaaactgcta acctacttcc
     9601 tgaaaatata gttcatgaag ttccagatac tcatttaaca ccagactctg gtaatactac
     9661 ggcttcaaga cagacagtat ttacgggaga agctactaag gttgcatcaa ataagttaaa
     9721 agaagcttta gactctggca aaactttact tgatttagag ggagaagagt tttatggaga
     9781 atatgaaagt attacagata aaatgggttg tgacaaagaa aatccagtaa gtcatgttgc
     9841 ttatggatat gcaacacaag ttgtaattct ggatgaaaat ggaaaactag aaaaagtagt
     9901 agcagcacat gatgttggaa gagctataaa tccaatcagt ttagaaggtc aaatagaagg
     9961 tggagtagtt atgggtcttg gatatgctct aaccgaagat tacccagtag tcaactctgt
    10021 tcctacagct aagtttggta cattaggttt atttaaatct acagatatac cagaaataca
    10081 agctttaata gttgaaaaga acaaagattt attggcttat ggggcaaaag gtgtaggaga
    10141 aatttgtaca gttcctactg caccagcagt acaaaatgcc tattatgtat ttgataaaga
    10201 atttagaact gagctaccac tacaaaatac tccatatagt aaaaaaaagt aacaaaaaaa
    10261 agaaatatta aataaaaaaa tagatgaaat tgtaaataaa aaaaaccctt ttgcataagg
    10321 tatattttaa atgatagaat atacatatac aggagggttt ttcttttata aaataagttt
    10381 aataaaattg agaggtgagc agaatgaatt ttaataatag tctagaatta actcagtctc
    10441 agaagctaat aatgactaca caacttaagc aatcattatc aatattaaat atgagtaagt
    10501 tagagttaga agaagagata aaaagagaag cagagaataa cccattagtt gaagtcgaga
    10561 aaagtgggga gataaattgg gaagagtata taaaagacat ggataggtca agacgtttag
    10621 atagaacaga gataagctat aatcctgata atgaagttaa tctagaaaat ttagttaaat
    10681 attcgtcaaa tttatatgaa gatttaaaat ttcagataag cctgtataaa cttacagaca
    10741 aagaattgga agtctgtgag tatataattg atagtttaga tgaggatgga tatttaagaa
    10801 ctgaggaaaa agtaattgtt gataccttaa agatagatga agaacttttt gaaaaatgtt
    10861 taattagtgt tcagcagttg gaacctagtg gagtaggggc aagaagtctt tcagaatgtc
    10921 tgatgataca aatggctagt ttaggaatat ataatgaaat tttagaggaa atcgttacaa
    10981 aagacttaaa tttaattgca aataataaat ataaagagat aagtaaaaag tacaatatgt
    11041 cattacaaaa atgtgttgaa ctaataaaca taattaagac attagacccc aaaccaggaa
    11101 gaacttgttc agtagagaaa agtgtataca ttcaacctga tgtaacagta gaaaaaattg
    11161 atgatgaatt tattgtatat ttaaatgaga aagattctta tcaaataaga atcaatagtt
    11221 attataaaga catattaaaa aattcacagt ctgatgaaag tgcaaaagaa tttataaaag
    11281 aaagactaaa ttcagcaaca ggacttatga aaaatataga gagtaggaaa actacagtac
    11341 ttaagattgc agaagagata gtaaaagctc aagatgaatt tttgagaaaa gggacaaaat
    11401 atataaaacc tttgaaaatg aaagacatag cagaaaaatt agaatttcat gaatctacta
    11461 taagtagagg tgtaaatggt aaatatatgc taacgccttt tggcgtatat gaatttaggt
    11521 actttttcag tagcgccatt gagactgaga ataatgaaat ggcatctagt acaagcataa
    11581 aaaaaattat aaaggaaaca ataaaagacg aaaataaaaa gaaaccttta agtgatgacc
    11641 atatatctaa gttattaaaa gaaaaaggaa tcaatgttgc aagaagaaca gttgccaaat
    11701 atcgtgaaga attaggaatc ccatcttcaa gtaagagaaa agaatactaa taagtaaaag
    11761 acaaatatca gtatagtaag gggaaaaggc taattaagag atgaatgtag attgggctaa
    11821 aaattttatg aagtaaaata atagaatatg gaaaagtatt aaaatgctat aaggtattaa
    11881 aaaactgggg aacattaaaa cattaagata gcaaagaaca taaaaaacta aagaagatta
    11941 aaacattaaa gaacattaga gctttgagga atattagaac tttaaagaat attaaaattt
    12001 ataaaatata aaaatactaa aaagaattga aaaactgggg aacattaaaa tattgtaaaa
    12061 tgctaaaaaa ctagaaaaca taattaaata agtgcttata aagaatgatt
//
LOCUS       DAEORZ010000124        12110 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEORZ010000124 DAEORZ010000000
VERSION     DAEORZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.125"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..924)
                     /locus_tag="KRH39_002942"
     CDS             complement(<1..924)
                     /locus_tag="KRH39_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBG8512829.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISLYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVIGLIIINAFLNKK"
     gene            1076..1516
                     /locus_tag="KRH39_002943"
     CDS             1076..1516
                     /locus_tag="KRH39_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8512830.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            1843..2070
                     /locus_tag="KRH39_002944"
     CDS             1843..2070
                     /locus_tag="KRH39_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG8512831.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            2096..4216
                     /gene="feoB"
                     /locus_tag="KRH39_002945"
     CDS             2096..4216
                     /gene="feoB"
                     /locus_tag="KRH39_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG8512832.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(4349..5167)
                     /locus_tag="KRH39_002946"
     CDS             complement(4349..5167)
                     /locus_tag="KRH39_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8512833.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            5374..5658
                     /locus_tag="KRH39_002947"
     CDS             5374..5658
                     /locus_tag="KRH39_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512834.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     repeat_region   5825..6053
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5895..5923
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(6712..8805)
                     /locus_tag="KRH39_002948"
     CDS             complement(6712..8805)
                     /locus_tag="KRH39_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8512835.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVKNPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
     gene            9458..10381
                     /locus_tag="KRH39_002949"
     CDS             9458..10381
                     /locus_tag="KRH39_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBG8512836.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            10356..11183
                     /gene="panB"
                     /locus_tag="KRH39_002950"
     CDS             10356..11183
                     /gene="panB"
                     /locus_tag="KRH39_002950"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBG8512837.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGTFPQEKHSFSINESELEKLY"
     gene            11202..12050
                     /locus_tag="KRH39_002951"
     CDS             11202..12050
                     /locus_tag="KRH39_002951"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBG8512838.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PDNIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         4511 a         1623 c         1556 g         4420 t
ORIGIN      
        1 tttcttattt aaaaatgcgt ttataattat taaaccaata actgttacat caaatataaa
       61 aaacagcaat aaaattatta tcaaaaataa aattctagca tataataatt ttctaaactt
      121 agctattata aagttttgta gtgacataca tgagtctcta atgataaaat agagacttat
      181 ataggttatt gatataaaga ttgtttttaa ataaacagaa tcaactttaa cattcaacag
      241 ttttagaact tcaaaaatgg ctagataaga aacacatatg taaaaagctt tttttgagca
      301 acataaaaat tggtttaaac ttctaaaatt cttaaatatt aaaaattttt ttaaagaatt
      361 agaatctaaa atatgcaatt tattccctaa aattaaactt aataaatata tgcaaaacac
      421 cataccaaaa ggaaacaatg ctatcattaa ataataaata aaatttgtat acccattaaa
      481 ataaatccat ttgctatatc cttgaaattc tttcataaaa ctataaccta ttaatgttga
      541 agcatatata tcaatgaaaa ccattacaat aaccccaaat actgaaccat atactaaatc
      601 atccataacc attgtaggct tatttattag atagccacac attataccaa gtaatacact
      661 ctgacttgcc caaatagcag ttcctagatt tccaagtacc agagaaatta ttaaaagcga
      721 agtaattcca gataaaatgg tatactttaa atcacatttc aagcagatta tacaaaaaaa
      781 tactggcact ataaaatcta agtaaatagc atatccaaat ccagtactca cagctataat
      841 tgttactact atgaataaag atgacaataa agctgcttca gttaattttt tagtagtatt
      901 caaaacactg cctccataaa tcataaattt tctaactaaa gttttgtttt taatatagta
      961 tatcactttt taaaaatatt tttaataaat ataaatttta ataaaggtat ttatgttaaa
     1021 aatggtataa tgtatcttaa tattcagttt tttaagatgc taggaggtaa ttactatgtt
     1081 taaaattaaa cactttaatg atttgagttt agatgaattt tatgaaatag caaaatcaag
     1141 atatgaggta tttgcttgtg aacagaagat attttcatta aacgattatg atgatataga
     1201 taaaagctct tatcacatat ttttaaaaga aaatggattg atttgtgctt acgctagaat
     1261 aattcctaaa gaatacagct cttataatga tgtttccatt ggtagagttt tagttctttc
     1321 tagtcataga agaaaaggat tagcaaaaca aatgatggat tgtgctatag attttataaa
     1381 agttaactta cacgaaaata atataacctt atcagcacaa acttatataa agaaccttta
     1441 cttatcttgt ggttttaaag aaatctcaga agtatacgac gaagcaggta ttgaacatat
     1501 taaaatgaga ttatagtgtt gacaagctaa tattgttaat atataattta agtataaatt
     1561 aaatattgaa ttgatattta ggtatttgaa aataccttac atagagtcag aaggcttttg
     1621 ctttctgact tttttattat caaaattcaa taattatatg gattcaaagt gttaattttt
     1681 attctatatt ctactttccc caaaagtaag aattataccc aactttgtat gataggcatc
     1741 ttgcctatct tttttttgta aaagtaacaa ggtaataata taatgcatag tttaaaataa
     1801 gaagattagc ccaggctaaa tttcaagcat ggaggtgcta ttatgaaaaa ccttaacgat
     1861 attcaagtga aaaaaactgt caaagttgaa gatatattat ctagtggtaa tttaagagaa
     1921 agaatgttag cgcttggtct tactagagga gctgttatag atgtagttag aaaaggtcca
     1981 aaaaataatt taactgtcta taaaatcaga ggtagcaaga tagctctacg acaagaagaa
     2041 agtagcttaa tattagtttc tgacatttaa aaatttctag caggaggata ttaatatggg
     2101 gttgacacat aactcaacta aaatgagttc tttaaaagac atgtttgata tagacaataa
     2161 ggaagaccag tttgtaattg cattggctgg caatccaaat acaggtaaaa gtactgtatt
     2221 taatcatctt acaggactta gacagcatac tggaaactgg ccaggaaaaa ctgtagctac
     2281 agctcgtggt aattttaaat ataaaaatac cgaatatgca ttaatagatt taccaggaac
     2341 atattcttta tttgccttat cacaagaaga aattgtagct cgtgatttta tatgttttgg
     2401 taatccagat gcagtaatag tagtatgtga tgctacatgt cttgaaagaa atttaaattt
     2461 agtatttcaa gtgatggaac ttacagataa agttattttg tgtattaatt taatagatga
     2521 agctagaaaa aaaggcatta ctattgataa aaaactttta gaggattctc ttggtattcc
     2581 agttatttta actgctgcaa gaaatggctc tggtatggat gaactattgg atactttaaa
     2641 tgatgtatct tttgacaaat acaaattaaa taataaacct gttagataca atgaaaatat
     2701 agagaatgtg gtaaaatcaa ttcaaccaga attggataat attataccag gtataaattc
     2761 taggtggttg ggacttagac ttattgatgg cgatgaaagt atatttgaat ctatgagtaa
     2821 ttatatagac aaagattcta tagatgctat taatgaagta aagaaaaaga ttccagacaa
     2881 tataaataaa cagaaaataa gagatgagtt tactaaaatc aactatgatt atgctaaaaa
     2941 actgagtgat gagtgttgtt ctaatgttgc taaaaaatca actgatagag aagaaaaagt
     3001 agataaaata ttaacatcta aaatatttgg gcttccaata atgcttttat tattaggtac
     3061 tatattgtgg attactatag aaggagctaa ctatccatcc actttattat ctaatttatt
     3121 actcggattt gagccaagta tttcgggtat attaaatagt attaattgtc catcatggtt
     3181 aaatgatatg ttggttttag gattatacag aacattagca tgggtaatct ctgtaatgct
     3241 acctcctatg gcaatatttt ttccattatt taccttactt gaggattttg gatatttgcc
     3301 aagagttgca tttaacttag accacctatt taaaaaagct tgtgcacatg gaaaacagtg
     3361 tttgactatg tgtatgggat ttggatgtaa tgcagctgga gttattggtt gtaggataat
     3421 tgactctcca agggaaagac ttatagctat acttacaaac aactttgttc cttgtaatgg
     3481 tcgttttcct actttgatag ctatatctac aatatttttt agctcagtta taaccaactc
     3541 tttcgtatca agcgtagcaa ctgcactttg cataacttta ctgataattc taggtgttat
     3601 aataacattg ttggtttctt atactctttc taaaacgcta ttaaaaggag ttccgtctac
     3661 atttacatta gaacttcctc cttatagagt tcctcaaata ggaagaactt tatatacctc
     3721 tataatagat agaacaatat ttgttttagg aagggctgta atggtagcca tacctgctgg
     3781 tgtcataact tggatatttg caaatatata tataggagat ttaagtatat tatcacatgt
     3841 tgcaaacttt ttagaccctt tagctaaatt aattggtctt gatggtttta tattacttgc
     3901 ttttattctt ggattcccag caaatgagat agttgtacct atacttttaa tggcttacct
     3961 agcaactggt tctatgatag aattagatag ctttagtgct ttaggtcaag ttcttagaga
     4021 acatggatgg acttatttga cagctttaaa tgttatgctg ttcagtttac tgcattggcc
     4081 ttgtgcaaca acacttttaa caataaaaaa agaaactggt agtttaaaat ggactgcctt
     4141 aggattctta atgcctacta tacttgcttt tgttgtttgt tttttaacaa ctacagttta
     4201 caatttattt atataattag tattaactga cagtagacat atattagaga aactatttaa
     4261 aaatttatat actttattat aaagcataga gatatattca atgataagta aaattaatta
     4321 acatttgaat atatctctat tttaattctt atctggatac ttggattgaa atttcagtca
     4381 tataattatc ttcattactt tcacaaaaaa agtcaagtat actctcctca tacgaatttc
     4441 caataatttt atattcattt tcatatatat attttttcaa tttttcgtaa gatttatatg
     4501 aatcctcata ttttccaata tgttttatgc aagcatacat tccttctggc tttacatata
     4561 gctttttatc ctttattttt ttatctatct tcaaataaaa acttgatata cttaaatagt
     4621 ccccattttt tatattttca ttacttacta ttactcccaa ctcatatcct tgatacaaat
     4681 ttttagattt acaataattg gtgaaatcta ttaattccat cataaaatca ctattactca
     4741 tattactcaa atcgcttgat agaactaaat attcttcaaa ctttttggat atataaattt
     4801 cttcactatt cattttagaa attccttcaa ctgttatatt tattctattt tgcatcttag
     4861 aacttatctt tcttagtttt tttatttttt ctcttatctt tttttcttct tctttaagca
     4921 ttttcacaca aagctgggga gttctatttt gtatatactc ttttatttcc tctaaactca
     4981 ttcctatttc tctcaatgat tgtaatatat caagtgtatc atattgataa actgagtaat
     5041 atctataatt atttttatct ttatattcag gagaaaaaat acctattttg tcatagaaaa
     5101 ttaatgtctg cttagatata ccacacaatt tagcaaattc acctgttgta aaatactgtt
     5161 tttccaaggg ttatacactc cttttctttt tgttgtctat atagttacta tattctttat
     5221 tatttactaa atcaagtagt tattatttta gaaaatcaat atggttttga ttttaaagct
     5281 taaatatcag ttagatgtca gttataaata ttttaccctt tcccatcata aaaaagatat
     5341 cccctcttaa cttttggatg gaatattata tttatacttg ttagagtgaa aattgtaata
     5401 ggtgctacaa taagatattt aattaaacat aaagttaatt attgtaataa aaaatcttta
     5461 gataaattag agttaaagta cggaaatata gatagagaaa aagctgttaa actagaaata
     5521 ttttatcagt atttaataga acttgaatat atagaaatga gtttattaat aagaagatta
     5581 gatactgcaa taacatcatt agcattagta tctattataa caatagcatc atattattta
     5641 atcagaaaaa atgtgtaatt atgtaagcta tttataataa aacactgata aaattaataa
     5701 taaaaaggta ataggatttc tatactacct tttggttttt ataatatagt gtaattttat
     5761 tatatttttg tatgctaata taactagata atcataagat aacacattaa aagaaaatta
     5821 taaaaactta cattccgtat agttaatata aaacataagt aatataaatt tatgtaaagc
     5881 aacaaaagat ggatttacat tccatatagt taatataaaa cctaaaaaac tttatagaga
     5941 ctttgaaaga ttcaataaaa tttacattcc atatagttaa tataaaacta gtttattaaa
     6001 aaacggtagc acaacaagtg ctgaatttac attccatata gttaatataa aacaccaaaa
     6061 taaacttagt attttcaaca cttacacgta tacaactctc ctaaatttgc agtgaacgag
     6121 caatagtgca attgataacg cttatcatgc actctcaacg ccttatattt caactgttac
     6181 gccaaatttt ttcacaaata tcgctcactg caaaatttct ataattttat tatatcataa
     6241 atatattact tttgaatatt gataccaatt tataccataa taaaagcaaa aaaacgtaat
     6301 ctatctacat tagatttaaa ttctataact taaagttttt tacataatat ttatgcttgt
     6361 ttaatcaatc taatatatca ttcttgtata ttgcttttaa tggtcttttt tcttaaataa
     6421 atatctatct gaacaagcat agtttaatta aaaacttttg tattccctta aaactgtatc
     6481 tacctcttat tattttattt atgatatatt atttttaatc taaaaataga tatctatttt
     6541 tattatggtt atcccttttt aaaatacttt aatcatatac tattcccaat ttaacatcca
     6601 ttgaaatgct attttcttat attgcaattt ataattgata tatcttcaat ataataattt
     6661 aatgtatttt tgattttaaa gcttttcagt gatattattg ataaattaga attaattatc
     6721 tagctttaaa aggtcaaaga tttctgttgc tgtctgcggt tttccaaata aatatccttg
     6781 tatgtaatct ggctttattt gtttaactaa atcatactct tcttgtgttt ctacaccttc
     6841 taaacatact tttattccta cgtcatgaca aattgcaaca ataaaatgta taaatgtagc
     6901 atcaaactta ctctttaata tatctttaac aaatactcta tcaattttca ctatatctat
     6961 tggagaaaat tttaaaactt ctagagacga gtatcccgta ccaaaatcat ccatagcaat
     7021 ataaattcct aaatcttgta aagccttaaa tttaaactgt aaaatactca tattttgtac
     7081 tgtatgactt tctgtcaatt ctaaaacaag attttgataa ggaaaatttt catcatctat
     7141 aatttttact atatcttcaa taaatgtgtc ttctaatatt tgaacagctg aaacgtttac
     7201 actcacttta aaaaaaggat agtaatcaat ccatttccta aaagtacgta atgccattcg
     7261 aagcacccaa gcccctacaa tatttatcat atcattttct tctaatattg gaataaattc
     7321 caaaggagaa attgctttgc atttgtcatt agtccacctt aaaagaactt ctacacctat
     7381 tattttatga ctttctgtat ctacttgtgg ctggaatcga agactaaacc ccctaaaatc
     7441 atcattaatt gatgccttta aatcccgcat catttctaat gaatacattt tattttttaa
     7501 tatttcttgg gagaaaaaaa caagcctgtt tttaccatgc tctttcgcat attgtaaaga
     7561 gtaactagca cacttataaa gttcttttac tgtatctcca tgttttggat atattacaca
     7621 accagcagaa attgttatat ttaatccata ttttctccat agttgaaggt gtataatcat
     7681 attctgtatt tgattatata gagttagaat ttcattttct tcaacattat caacaagaat
     7741 gcccatttcg tccccatcaa gtttataaag ttcagcatta tctggtaaaa tagattgtat
     7801 agactgtgct agtgttttta ttatattatc tccaaaatcc ctatcataca tctcattaac
     7861 atttttaaaa tcgtcaatat ctaataatac tatgcataat ttttcaatca taggatttga
     7921 aattttatct tcaaatacac tcataaattc atgataattc agtaactgtg tcaatgaatc
     7981 aatcttattt tgtttaccta attgcgtcat tattcctgca aaaatacttg gttctccaaa
     8041 ttcatcacgc attagttgac ctctacatct taaccatata tattctccac tacgattttt
     8101 agccctaaat tccactgtat gataatccat ctggttttta cctatttcag tgtttgactt
     8161 ataaaaacga ttccagtctt ctggatgtac aatttttttc cagtaaacaa gcggattttt
     8221 aacaatttct cctggtaagt caaataattc aacttgtgat ggtgaatatc gaaatacgcc
     8281 tgttttcata ttgcaaatgt atatatattc atctgtacct ctaatcaaag catcgtaaag
     8341 caatgtagaa ttataatcaa aattgtctct tgaaaaatta aaaactcctt tttcttcttt
     8401 attatttgat atttcatgaa attcattaat cttaattggt ttatttaagc ttgttgagtt
     8461 tgcaatatct attgattgat gcatatgaac aactgtgcca tcttgccatg taattaaact
     8521 atcataattt tcaaaaacac gatttaactt attacatttt tcataccact ttattaatac
     8581 tcctttttta tcattcttta ataattctaa aactttacaa aaactacaag tagaattttt
     8641 ttcaggatat aatacttgcc aacaaacttt tccttctgga tctaaaatat tatattcttc
     8701 tttcattttt ttgttcataa aaataatttc attagtatga atatttgtaa catatatatt
     8761 aatctgtagt tgatttaata taacttcaaa attatgtttg ttcatatact tatctcctcc
     8821 atagttactg ctctttataa caacattgtt aaaattattc tttttagaag atttacttat
     8881 aacttataca tatacactaa ataccttaat ttaattagaa agtttattac ttcatatttt
     8941 aaattataat tgataaaaaa aaatacagca atatagtatt accaatttaa aaaaaattaa
     9001 tattacataa tacatctctc tcttatatta aattttaact tggtactaat cttgacaaat
     9061 tgtccacaaa aataaattat atattttatt attgttttaa atttttataa aaaatcagta
     9121 ttattgatac tattgaagtt aaaaagcatt aatttctaaa aacatcataa tttttctaat
     9181 agacttaatc acttttaata tatatagtaa aacaaaaaac ttatatttta taaatatcat
     9241 aattttttta ctgatatttt attatataaa ttttatagaa ttaattgtca attttttatg
     9301 aaaatggtaa gatatttatg gatggtatct tgttataaga actatacttt aaatgttttt
     9361 aattattttg tcttatgtaa ctcatatcct caggaatgag attagaaatt aagatgttgt
     9421 atcacaaagt ggaggtgata ctattaaaat aggctttatt ggttctggaa aagttggaac
     9481 atctttggca aattattttt tatcaaaagg ttattgtgtt tcaggctttt atggaaggaa
     9541 tcaattatct ttattagaat caacaaattt aacaaaaaca aagatttaca gcaatttgaa
     9601 agatataatt tatgaaaacg atatattatt tattacaaca tcagatgatt ctattgaaat
     9661 tatagacaaa aaactttcca aatttaattt aaaacataaa tacatttgcc acacaagtgg
     9721 ctctttaaaa tctagtatat tgttctgttc aaaaaaagca ggtgcattaa tttattctat
     9781 ccatcctata tttgcatttt ccaataaaaa taccaacata aaaaaaatga aagacatttg
     9841 tttttccata gaaggtgata atgaacaaga tgatttagtt attcaaaatt tcatagatga
     9901 tttgggaaac caatttttta taagagataa ggatacttca tccacttatc atttagctaa
     9961 tgttttagtt tcaaatttag tattatcttt actagatata ggagtaagtt actttattaa
    10021 attaggttta tctgaagagg aatctttaaa agctatcagt cctttagtaa aaaaaaatat
    10081 agaaaatata cttgataatg gctttactaa agctcttact ggtcctgttt ctcgtggaga
    10141 tataacacca ataagaaaac atctatctgt attggataaa aaagatgagg atatttacaa
    10201 aatactatcc ttaaacctac taaaaataat tgccttagga aatgacaatt cattaaaagg
    10261 tataaaaaat attactatag aaaatgcaat cgaaaattta ataagtaaat ctgaaaaata
    10321 caaagaaatt tacaaacttt taggaggaaa aaacgatgaa aaataccata cagactttta
    10381 agaatgctaa atatgaagga aaaaaactat ctatgcttac tgcttatgac tactcaattg
    10441 caaaaataat ggatgagtgt gatataaatg gtattttaat aggagattca ttaggaatgg
    10501 tcataaaagg tgaagaaaac actctatctg ttaccataga cgaaattata tatcatacaa
    10561 aagctgtaaa aaatggtgta aaaaatgcct taatcgtaag tgatatgcct tttctatctt
    10621 accatgtttc tatagaagat gcagttaaaa acgctggtag acttataaaa gaaggaggag
    10681 ctcatgctgt aaagttagaa ggtggctcaa acgttataaa gcaaatagaa agcattgtaa
    10741 atgctcaaat acctgtaatg gggcatttag gattaactcc tcaatcagta aattcatttg
    10801 gtgggtttaa ggttcaagga aataccagtg aaacagcaag acaacttatt gaagatgcta
    10861 aattaataga aaaagcaggt gcattctcta ttgtccttga aggagtgcct acgaaaatag
    10921 ctgagatggt aactaattct atttcaattc ctacaatagg tataggggca ggaattaact
    10981 gtgatggaca aattttagtt tatcaagata tgttgggaat gtttggagat tttgttccta
    11041 agtttgtaaa gcagtatgca aatataggag atataatgaa agattcaatt aaaaattaca
    11101 tcctagaagt taatacaggt acatttccac aagaaaaaca cagcttttca ataaatgaat
    11161 ctgagttaga aaaattatac taatattaag gatggtacaa aatgcttgta aaagaaatta
    11221 aattactaag aaatataatc aaagattgga gaaaacatgg ctattctata gggcttgtta
    11281 caacaatggg atttttacat gaaggacacc aaagtcttat aaaaaaagct gtaaaagaaa
    11341 atgataaagt agtagtgagt gtatttgtaa atccaactca atttggacca aatgaagatt
    11401 ttaatagcta tccaagagat atagataaag attttaaata ttgtatggat agtggagcaa
    11461 cagtagtctt taacccatca ccagaagaaa tgtatttaaa aggtaattgt actaccatta
    11521 atgtttctgg acttacagat tttctttgtg gagctaaaag acctgttcat tttggaggtg
    11581 tctgcctagt tgtttcaaag tttctaaata tagtaacacc tgataaagct tattttggag
    11641 agaaggatgc ccaacaatta gctgtaatta aaagaatggt caaggattta aacatagata
    11701 cagaaataat aggctgtcct ataattcgtg aaaatgatgg attagcaaaa agttcgagaa
    11761 atacatatct ctctgaggaa gaacgaaaat ctgctctaat attaaataag agcttaagtt
    11821 tagcaaaaga agaattagta aaaggcaatc taaatccgga taatataaaa gaattaataa
    11881 cagctaaaat aaattctgaa cacttagcaa aaatagacta tgttgaaatt gtagatagtg
    11941 agacattaca acctgttaag caaatagaac attctatact tgtggcaatt gctgtgttta
    12001 taggaaaaac cagactaatt gataatttta catttgagtt aaatatatag tctataattt
    12061 tatataatat atctaaaaaa taatagaata cctaaaaagt aataaattac
//
LOCUS       DAEORZ010000125        12071 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEORZ010000125 DAEORZ010000000
VERSION     DAEORZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.126"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..887)
                     /locus_tag="KRH39_002952"
     CDS             complement(<1..887)
                     /locus_tag="KRH39_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG8512839.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMG"
     gene            complement(918..2636)
                     /locus_tag="KRH39_002953"
     CDS             complement(918..2636)
                     /locus_tag="KRH39_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG8512840.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDSIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            complement(2862..4100)
                     /gene="fabF"
                     /locus_tag="KRH39_002954"
     CDS             complement(2862..4100)
                     /gene="fabF"
                     /locus_tag="KRH39_002954"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBG8512841.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            complement(4129..4353)
                     /gene="acpP"
                     /locus_tag="KRH39_002955"
     CDS             complement(4129..4353)
                     /gene="acpP"
                     /locus_tag="KRH39_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG8512842.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            complement(4407..5156)
                     /gene="fabG"
                     /locus_tag="KRH39_002956"
     CDS             complement(4407..5156)
                     /gene="fabG"
                     /locus_tag="KRH39_002956"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBG8512843.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            complement(5156..6106)
                     /gene="fabD"
                     /locus_tag="KRH39_002957"
     CDS             complement(5156..6106)
                     /gene="fabD"
                     /locus_tag="KRH39_002957"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBG8512844.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            complement(6122..7051)
                     /gene="fabK"
                     /locus_tag="KRH39_002958"
     CDS             complement(6122..7051)
                     /gene="fabK"
                     /locus_tag="KRH39_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBG8512845.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            complement(7160..8146)
                     /locus_tag="KRH39_002959"
     CDS             complement(7160..8146)
                     /locus_tag="KRH39_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBG8512846.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            complement(8172..9194)
                     /gene="plsX"
                     /locus_tag="KRH39_002960"
     CDS             complement(8172..9194)
                     /gene="plsX"
                     /locus_tag="KRH39_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBG8512847.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            complement(9205..9762)
                     /gene="fapR"
                     /locus_tag="KRH39_002961"
     CDS             complement(9205..9762)
                     /gene="fapR"
                     /locus_tag="KRH39_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBG8512848.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            complement(9969..10145)
                     /gene="rpmF"
                     /locus_tag="KRH39_002962"
     CDS             complement(9969..10145)
                     /gene="rpmF"
                     /locus_tag="KRH39_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBG8512849.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            complement(10170..10694)
                     /locus_tag="KRH39_002963"
     CDS             complement(10170..10694)
                     /locus_tag="KRH39_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBG8512850.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            complement(10787..11983)
                     /locus_tag="KRH39_002964"
     CDS             complement(10787..11983)
                     /locus_tag="KRH39_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBG8512851.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
BASE COUNT         3545 a         2395 c         1252 g         4879 t
ORIGIN      
        1 cccatagaaa aaatactttt tttatttaca tttatatatt ttttatttgt aaataccact
       61 tcatcaatta tttctttgtc ttcaagtaaa atatttaaat cttcagaaat aatatcttct
      121 atgtttttgc cttgtggatt ttcatttata cctaaaattt ttaaagcttc tttattaaat
      181 gtgtttatta taccatctgc tgaagtaaat attattccat cttttgatag attcaatata
      241 acatcttgtt cttctatttt taaaaacagt tgcctataat tatctttaac tccattatct
      301 aaacttatta aactctcaga atatgccatt aaatttttac gtatttcttt aacatctatt
      361 ctaagaccat ttattatttc tataaaggta gatatatcaa tatatctatt tccaaggtcg
      421 attactttat ttatataact tggtactttt ccctttactc caggcgttat agctatgtct
      481 atatttttat aaatttttcc ttcaatatat ggagtaaact ttaaattttt tactcctatt
      541 ttgtaaaata agcttatcgt ttctaaggta gtttcttttg tatcatttac aaccaatact
      601 tcagttccat ttggtattga aaataatcca tatatttcat tttctttaag tgttctcctt
      661 atcactataa tcttactatc ttgtactaaa tgctttctca tactgtttaa tctatcatca
      721 ttcattacta gtacaaaatc accttgtatt ttatcattct cttttaaaac ttccagataa
      781 tagtggttta tatcaatata attacctaaa accaaacgta tattttcttc taagaaagat
      841 gatagtttag ttattttatc agttactata gctattgatt gtttcatatt tatacctctt
      901 atttatatag tatataatta cttatatact actttatttc taccaagttg ttttgcttta
      961 tataaatttt tatcagcttc ttctagtaca tctttgctat ctgaaatatg agctacacct
     1021 gcacttatag tcacagtcac atcatcattc cattttttat ttgcaattgc aacccttata
     1081 ctgtcaatta tatctgctac ttcctcacca gatttatttc ttattaatac agaaaattct
     1141 tctcctccgt acctaaagac tttatcatct tgttctaaca taacacaaat ggtatttgtt
     1201 attcccttta taacagtgtc accaaattta tgtccataat tatcatttat gagcttgaaa
     1261 ttatcaatat ctatcatcac aagtgcaaaa ttatcatctt tctctctgta cttttcatac
     1321 ataatatcaa aactttttct attataaaca tttgtaagac catcatgtaa attttgtgct
     1381 ttaaaatact tcaatcggtt gtataaaaaa gtagacccta atatagcaat taaaattact
     1441 gtaaataata ttttataata ttttatttct tttttagttt tttcaaatag cttactttcg
     1501 tttgatactt tatcaattat atagaaacta gtctctgaat ctcttatact tttgttctct
     1561 ttatattcat ttattaacat cttttgatac tcatttaaag attctatatt tccttgtttt
     1621 tcatatatat ttaatatctt atttaaattt tttattttct cctgggttaa ctttcttttg
     1681 gtagatatct ctaaactttt tttataatta tctattgcag aattataatt ttcgctttta
     1741 tattctaagt ttcccattgc catataatag tacatatctt tatctaatat ataaactgtt
     1801 ctatctgatt ttataagata atcagctttt tctaaaaaag tctttgcctt ttgtatatta
     1861 tttttgccca catacgcatt tgactgcatt atagaggcca ttatatcaaa atcattgtaa
     1921 ttatccgtat ttacaaactt tttataatct tttatcctgc tacttatttc aatagcttta
     1981 tcatattctc cctcttctac atatatttct gctagattta taatagcata taatctaacc
     2041 attccatttt tttcaccttc atcaatatct attttaaaag aatcctttat taatttctct
     2101 cctgtttcat agccacctat ctttaaaaat atgctcgcca aatcaacatc tagtcttgct
     2161 ttttcataat tattccccaa tttctcagcc aactttatag actttacagt taaatctact
     2221 gccttagaat aattattatc cattatatac actgatgcta atcttttttc tatattaaat
     2281 ctacattcat cagataattt cacatctgat tctctcatct tttctaaaga atcaatgact
     2341 atcttcgttc catctggaac atatgaaata gtctttaaca tatcccaaac ttcattatat
     2401 actttattgt attttttttc tggaatagat tcaaaagaag tcttaaccac tttttttgct
     2461 tcatcatact ccttatctgc cacaaggctt tcagacatat attttccctt atttataatt
     2521 tctctttctt tcaatccatt ttggtcatac tttataaaca aaataatact aataagtatt
     2581 atacatataa aaattatctt tttgggtaac cttttctttg gtagtctaaa attcaaaatt
     2641 ttagtcctcc ttttgtatat tgtagaatga aaacttgcat atataaataa cctcttaata
     2701 attaataata caatatatca aaatgattta caacatatcc tagacttaaa tcttaataaa
     2761 aaagtctcct aaaataattt aggagacttt aaatttaagc taatataaat ttatacagtt
     2821 aaaacaagtg cttgtttttt agttttatat ttaatattta tttatacata tttttttagt
     2881 actattgtag cattatgccc accaaatcct aaagaatttg atagtgcata tctaatattt
     2941 ttttctttac cttgatttgg aacataatct aaatccaaat cttcaccaac ttcttgtatg
     3001 tttatagttg gaggcacaaa actatcttct atactcatag cacaaacaac agcctctata
     3061 gcacctgatg ctcctaataa gtgaccagtc atagattttg ttgatgacac acataagtca
     3121 tatgcctttt ctccaaatac agtttttata gccatagttt catataaatc attgtaataa
     3181 gttgaagttc catgagcatt tatataatct acttcttcta atggaacatt tccatctttc
     3241 aaagccatat tcatagacct agctgctccc tctccatttt cagctggagt tgtcatatgg
     3301 tatgcatcag cagttaaacc atatccaact acttctgcat atattttagc gcctcttgct
     3361 aaagcatgtt ctaagtcttc taagataaca atacctgctc cttctcccat tacaaaacca
     3421 tctctgtctt tatcaaatgg tcttgatgca gttttagggt catcatttct agttgacata
     3481 gccttcatgt tacagaatcc agcaaaagca agacctgtta cagcagcttc agagccccct
     3541 gctaccatga tgtctgcatc acctctttga attaccttaa aagcatcacc tattgaatga
     3601 gttcctgatg cacaagctgt acatacatta gtgttaggcc cttttgctcc taaaaatatt
     3661 gaaacttgac cagctgccat atttcctatc atcataggta caaaaagtga acttactctt
     3721 ttatttcctt tttccaaaag aattttatgt tgagtttcta tagcttcaac tccacctatt
     3781 ccagaaccta ttataacacc aaaacgttct ctatcaactt tatctaaatc aagatttgca
     3841 tcttcaactg ctaatttagc agatactatt gcaaattgag caaatttatc taatctcttc
     3901 aattcctttt tacttatata ttcttctgga tgaaaattct taacttctcc tgctatctga
     3961 gtttgatatg tgctagcatc aaaattagtt attttatcta taccagatac tccagatttt
     4021 atattactcc agaactcttc ttttcctgtt cccaacggcg taacacatcc aagtcctgtt
     4081 attactactc ttttattcat cggatacctc cactttttta aagctcgact atttattagc
     4141 ttctatatac ttaactatat ctcctataga tttgaagttt tctgcttctg tatctggtat
     4201 ttctatacca aattcatctt caagtgccat tattatttct actgcatcta atgaatctgc
     4261 ttctaaatcc ttcattaatg atgtctccat agttatttct ttatcttcta ctcctaattg
     4321 ctctattatt atttctacaa ctttattaaa cattgtgtac ctcctaaaat tttttatttt
     4381 aatagatttc tctacttttt aaattattat tgcattacca ttccaccatc tacatttatt
     4441 acttgtcctg ttatataact tgacatgtca ctaactaaaa atgctacggc attggctaca
     4501 tcttctggtt taccataaga tttctttggt atgtttgcta gtgttgattc ttttacatca
     4561 tcacttagta ctttagtcat atctgtttct ataaatccag gtgctactgc atttatattt
     4621 atgtttcttg atgctaactc tcttgcagaa gcttttgtaa atccaattac acctgctttt
     4681 gatgcacaat aattagtttg ccctgcatta cccataattc caactactga agtcatattt
     4741 attattcttc catacttctt tttcatcata ggtttagtaa ctgcttttgt acaattaaag
     4801 acacctttta agtttatatc tataactcta tcaaaatctt cttctttcat tctcataagc
     4861 agaccatctt tagttatgcc tgcattatta accaatatat caatttttcc aaattcgctt
     4921 acaacagaat ctatcatttg attcacttca tcaaatttag atacatcaca ttttatagag
     4981 gtacacttta cccccatact ttctatttca tttttagttt ttagtgcttc atcttcttta
     5041 gaagtataat tgattactac atcagctcca aaagatgcta gtttttttgc tatttctttt
     5101 cctatacccc tagagccacc agtaacaact gctacttgtc ctgtaagatt tatcattata
     5161 tctctccatt tctataattt tgtacaaatt cattaagaga atctatacca tctatattat
     5221 aaatattgac atttgctttt ttattggaag ctatcttctt aataaatgct tttaaagttt
     5281 ttccaggtcc catttctata aaagtatcat atccatcatt taaaagtctt tctatagaat
     5341 cttcccaaag aacagatgaa cttacttgtt ttataagcag gtctttacct tcgtttttat
     5401 agtaatctgc atttacattt gccaccacat caactttagg tttagttatg ttaacttttt
     5461 ctaattcact ggccaatttt acaccagctt cttttaacat tgatgagtga aaaggtccac
     5521 ttacatttaa tttaactgcc ctttttgctc ctgcttcttt gcattttacc attgcttctt
     5581 ctatcttttc attttcacca gatataacta tttgtccagg agaattgtaa tttgctactt
     5641 ctacaatacc tccttgtata tctttaacca cattttcaag tacatttcta tctaaaccta
     5701 aaattgctgc catagctcca acacctgctg gaactgtttc ctgcataaac ttacctcttt
     5761 ttttaactaa acatacagca tctttaaagt caattgcatc tgctgcaacc aacgcactat
     5821 actctcctaa ggataatcct aagcatgcat tatattttaa atctatttga gcttgtaccg
     5881 cttttaatac tgctacacta tgtgtaacca tagcaggttg agtgttttca gttttagaaa
     5941 gttcttcttc atttccatta aacataaggt tttttaaatc catatttaag gcatcactag
     6001 cctcatctat tacactttta gcaacagtat tattttcata taaatccttg gccataccta
     6061 catattgagc tccttgacca gggaatacta gtgcaacttt tcccatcttg ctcctcctga
     6121 tttacagttc tattgctggc ataatctctc ttgcttgatt taccatagcc tctataattt
     6181 ccttacaagg tgttatttct tttaccatag cagcactttg acctgccata aatgaacctt
     6241 tttctatgtc tccatctact gttgcaaatc ttaaagctcc tgtacctttt ttatccagtt
     6301 cttcaggagt agctccattt tgttccatct ttaaaaattc ttttgatagt ttatttttta
     6361 atgttcttac tggatgacca gtacttcttc ctgttacaat tgcatctcta tcttttgctt
     6421 ttagtactaa gtttttatag tttgaatgga cagaacactc ttcactgcaa ataaatctag
     6481 ttcctacttg aactgcactg gcacctaatg caaatgatgc tgcaattcct ctaccatcta
     6541 caattcctcc agcagctatt acaggtatgt ttacagcatc agcaacttgt ggaactaaga
     6601 ccatagtagt aagttctcct atatgtcctc caccttcagt accttctgct attactgctg
     6661 tagctcctag cttttccatt ctttgtgcca aagctattgt aggtactaca ggaataacct
     6721 ttgttccagc ttcctttaat ctatccatat actttgcagg atttccagca ccagtagtaa
     6781 taacttgaac tttttcttct ataatcaaat caattatatc atcaacaaat ggcgacataa
     6841 gcattacatt tactccaaaa ggtttatctg ttaatttttt acactcaaca atttctttct
     6901 ttatagcttc ttttggtgcg tttcctgctg ctattatgcc aagtcctcct gcattagata
     6961 cagcacttgc taatgatgca gtagctaccc atgccattcc cccttggata actgggtatt
     7021 ttatatttaa tattttgcaa attttattca tacaattctc cttcacttga aatcataaaa
     7081 gtaaactttt tattattaat caactttatt ttgtgtataa gtttaatttt gttaattaaa
     7141 tcattttaga tattttatac tataaagtcc acttgacaac tgaagccccc caagttagac
     7201 ctccaccaaa gccaactaaa actacattat cacccttttt aattttacct tctctataag
     7261 cttcatctaa tgctactggt atagatgcag cagacatatt tccatatttg tctatattta
     7321 cataaacttt tttcatatca agctctaatc tcttagaaga agcttgtatg attcttatat
     7381 tagcttgatg tggcactaag tagtctatat cagttgtatt tagatttgct ttttcaagag
     7441 ctcttaaaga agtttctgcc atctttctaa ctgcaaattt aaacacatca ctacctgcca
     7501 tttctattgt atgtagatta gcttctacag tctctttact tgccttaagt cttgaacctc
     7561 cagcaggtac ttttaaacaa tcttttcctt taccatcaga gcccatatgt gtagacagta
     7621 ttccaccctc atttacaggg ccaataacag ctgctccagc tccatctcca aacaatactg
     7681 ctgttgttct atcatcataa tttaaaaact tagaacaagt ttctgctcct attacaagca
     7741 catttttata gcaacctgtt tccacaaatt gctttgcaat agttactcca tatacaaatc
     7801 cagaacaagc tgctgatata tcaaatgcag tagcattcac tgctcctatt gcatcttgta
     7861 ctatacatgc agttgaaggc aaagacatat caggagtaat tgtagctaat ataatcagct
     7921 ctatatcctc tacgtttaac tttgcacatt ctatagcttt taaggcagct ttagatgcta
     7981 agtcagaagt tgcttcagac tctttggcaa atcttctttc cttaatacca gttctagtac
     8041 ttatccactc atctgatgta tccattattt tttcgaagtg aaaattatca tatgattgct
     8101 caggtagata acttcctact cctaatattc cagcctttgt attcattata tattaaacct
     8161 ccaaaacgag tttattcatc ttcgttattt ttattttctt cattgtattt gcttataaat
     8221 tggttaatat cttcaacgac ttttcccttt gaaaatttta ttccttgatt tatagcattt
     8281 tttatagctt tagcatcaga actaccatga gcttttataa ctcctccatt tagacctaaa
     8341 agtggagctc ccccatactc agaataatct ataaaacttt ttaattttct taaatcatcc
     8401 tttataagta atgctcctat tttacttttt gtactggcca taaaagtttc ctttataaga
     8461 ctcataactg aaagtgctac accttcagca gatttaagta atatattacc tgtaaatcca
     8521 tcacatatga ttatatctgt gtatgcattt ataacttctc tagcttctac attccctata
     8581 aaatttaaat ctaatttttt aatttcttca tatgaccttt tgactaaatc attgcctttg
     8641 ccttcttcta gtccaacatt tgctaatgct accttaggat tttcaagacc taaaactttt
     8701 cttgaataaa tattactcat agctgcaaat tcaactagat tcttaggttt acaatctgca
     8761 tttgcgccac agtctgctat aagtgtcatt cctcgcttta catttggtat tgctgaacat
     8821 aagcaaggtc tatcgattcc ttttattcta ccaacaacaa ataatccacc agcaagtaag
     8881 gctcctgtat ttccagctga tattatagca tctgcctttc cttcttttac aagatttaat
     8941 gctactacca tagatgaatc ttttttactt cttatagcct ttactggttt atcttcgttt
     9001 tctataatct cagtagtatg aactatctct aatttatttc tatcaaattc ataatttgaa
     9061 aattcctttt caagtaaatc tttatctcct gtgattatta aatcaacctg atattctttt
     9121 atagcattta ctgcgccttc tacattagat ttaggtgcat tatctccacc cattccatca
     9181 attactattt tcataatttc cctcctaatc taattcttca aagataaact ttcctctaaa
     9241 tatttgctct tgggctttat tgttgataac aacttttaca taatgctttt tatctatatt
     9301 tctatacact tctgcttttg ctataagtct atcttgttgt tctatcaact taaaactttt
     9361 tacatttgca actctcataa taaccttagg tgcatctatt atagccattg ccaatgaatc
     9421 tgctagagaa aaaatatacg tatcttttaa agtattagtc ttagagtaaa tcatgtcctg
     9481 tgtaacttct aacatagata tacctaatcg ccctacagat aaatcaatga tttctcctgt
     9541 aatttccttt actcctaaag tctttacttt tgaactctga gtttctgcta ttgatttgac
     9601 tctttctctt agctctggta tgtttagact cattctatct aatcttatgg tctgtatact
     9661 tacatcaaac agacttgata attcttcatc agtataaaaa ggatctgtct ttagcatatc
     9721 tataagctct ttttgtcttt gggctttact tttctttttc atgttccctc caaattttat
     9781 agctgtaatt agtaccagct actaatagta gtatatagta acacacaaaa attttcaata
     9841 cttatattat tatttttcta ttttttaata tgcataaaat ccacttattt tctctagtaa
     9901 atcatcttat ttttttatat gtacaaaaaa aacatgtaga gtatactcta catgcttttt
     9961 agaagctatt acttagacac aacttcttta cctttataat atccacagtc tggacacact
    10021 ctatgtggta attttggctc atgacattgt gggcaactta caaatcctgt tgcttccatc
    10081 tttgagttag ctgctcttct catttttgtg tttgatttag acgttttacg ctttggtact
    10141 gccatttacg ccacctcctt agtcaaaatt taattaaata aatcttttaa tttagcaaaa
    10201 cgaggatcta tgtattcctc atcgtttgta atttcactgc aagaacactc ttcttcattt
    10261 aagtttgccc cgcatacttg acaaagacct ttacagtttt cactacaaag aactttatga
    10321 ggtacattaa aatctaatgt ctgttctatt atatcaataa ggtcgatttc ttcaccatcg
    10381 aatataaaag gatcaaattc ctcaaactca tcttcatcat aatcttcttc ttttactaaa
    10441 aaaccttgta tagaatactc tagtggtatt tctacttctt taagacatct tgaacaattg
    10501 tcaacaattg taaaattgac atctatatct agatacaaac ctttattagt ctttgacact
    10561 ttccctttta aatttacagg tgatactaat ttgtaaatct catcacaata actaatagta
    10621 tcaatttttt cacaaaaatt caagtctatt ttgtcagttt cttttctatt tattttttct
    10681 atgctaactt tcattaagtt cacctcaaca tatcaccaaa tttattatac aaatctagca
    10741 tttgtttgtc aagtattttt acttgatagg cataggttta atttaattat tttaccaatc
    10801 ttaatgtatc tctagctatc attaattctt cattagttgg tattaaaagc atttttactt
    10861 tagaatcagc agtagatatt actgtttctt ttcctctaac atcattagct tctttatcta
    10921 atttcattcc taaaaactcc atgttagaag caatatctgc tcttatagcc tttccatttt
    10981 caccaacgcc tgcagtaaat actacaacat ccaatccatt catttcagca gcataagcgc
    11041 ctatatattt ttgtactttt ttaacatacg cttctaatgt agcttgagct ctttcgtctc
    11101 catttttagc tgcatcttct atatctctga agtcactaga aattccagtc attccatata
    11161 ctccagattc tttattcatt aatttatcca atccatcagc atctaaacct tcttttctca
    11221 ttaagaatgg taatatagct gggtctatat ctccacatct agttcccatt attaatcctt
    11281 ctaatggagt gaatcccata gatgtatcta cacattttcc accatcaaca gcagctatag
    11341 aagcaccatt tcctaagtga caagttacta tttttaaatc ttttatatct ttccctaata
    11401 tctctgctgc tctttgagat acataattat gagaagttcc gtggaatcca tatcttctta
    11461 ccccatattt agtatatagc tcatgaggta aaccatataa gtatgaagat ttaggcattg
    11521 tttgatggaa agcagtatca aatacaccta ccattggtac atctggaagt atagctttac
    11581 aagcatctat tcccattatg tttgctggat tatgaagtgg agctaaatct atacattctt
    11641 ttaaagcttc ttctacttct ggagttatta ctactgaagt tgcaaatttt tctccagcat
    11701 gtacaactct atgtcctact gcatctattt ctttcatttc tttaactgca ccaaattctg
    11761 gatgagcaac agcttctaaa actaatgcta ttgcatcttt gtggtccttc attggttgtt
    11821 caacaacgta tttatcatct ctacctgctt tttcatgttt taatatagaa ccttctattc
    11881 ctattctttc aaccaatcct atacataaaa cttcttcatt attcatatct attaattgat
    11941 attttaatga agaactccca cagtttaata ctaatatttt cattattatt tcctccttag
    12001 atttttttat aaataatatg taataaattt tgatttgtaa taaatgtcta aataaaactc
    12061 tatcttttat t
//
LOCUS       DAEORZ010000126        11976 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEORZ010000126 DAEORZ010000000
VERSION     DAEORZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.127"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            60..1064
                     /locus_tag="KRH39_002965"
     CDS             60..1064
                     /locus_tag="KRH39_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8512852.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            1190..2302
                     /locus_tag="KRH39_002966"
     CDS             1190..2302
                     /locus_tag="KRH39_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8512853.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAES
                     KQNESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            2591..3352
                     /locus_tag="KRH39_002967"
     CDS             2591..3352
                     /locus_tag="KRH39_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512854.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            3355..4173
                     /locus_tag="KRH39_002968"
     CDS             3355..4173
                     /locus_tag="KRH39_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBG8512855.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQEEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            4353..4955
                     /locus_tag="KRH39_002969"
     CDS             4353..4955
                     /locus_tag="KRH39_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBG8512856.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    5465..5702
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5766..7685
                     /gene="thrS"
                     /locus_tag="KRH39_002970"
     CDS             5766..7685
                     /gene="thrS"
                     /locus_tag="KRH39_002970"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBG8512857.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            8451..9542
                     /locus_tag="KRH39_002971"
     CDS             8451..9542
                     /locus_tag="KRH39_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG8512858.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIVEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKELIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     VFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSSLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
     gene            complement(9764..10801)
                     /locus_tag="KRH39_002972"
     CDS             complement(9764..10801)
                     /locus_tag="KRH39_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG8512859.1"
                     /translation="MNKKLIKAKIFIWSILAISLSIVFVSLLIGTIISHSFNFPFNIL
                     NPKYNYDNYIVAKNESFSDKINDINVDWISGEVRIKRSDSNKIKLIQKIPSNFSKKKL
                     AKIMVDGDSLSIYDNSAKKFFIGINLPKPTILELYLPNKLYDKITLYTTSADIYSTYI
                     RSNTVEANTVSGAIGLSGNINKINLDTTSGHIDVAKLNSKNATLKSTSGEVQLSGKID
                     VLNLETTNGDINLKDIYNKNLTCSSVSGKMNLSGVFSDIKAESTSSNIKITSSKMLSS
                     LYCETTSANVDLSIPDNPGFTLNFDKVSGVLDSDFELNRHSDLYTYKNGITDLYLSTT
                     NGNLTIIKNNH"
     gene            complement(10794..11480)
                     /locus_tag="KRH39_002973"
     CDS             complement(10794..11480)
                     /locus_tag="KRH39_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512860.1"
                     /translation="MLDKIRTYIDSSFTGVNETKKVKELKDELFENLKEKYNDQIQDG
                     KTEQEAYNSVISGIGDLSELIESVKEPYSLPSELIEEKKKRALRASIAVALFIISPFL
                     FVVSVVSFGIEPTIAILLMLILIALGVGLLVYNQMTRTNYALYNDNLSNKFKEFEDFE
                     ENYNHINPVYKSFMNAYWIIIVSIYLITSILFNIWSFSWILFIIGAAGVNIVKGIIQL
                     KGYKEEDKYE"
     gene            complement(11492..11812)
                     /locus_tag="KRH39_002974"
     CDS             complement(11492..11812)
                     /locus_tag="KRH39_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8512861.1"
                     /translation="MIIASDMIRGHTETIILKHLLEKDSYGYEINKSILEKTKGLYEL
                     KEATLYTAFRRLEQSGAITSYWGDEVTGARRKYYSITNIGKEKYIKSREEWNNTKALI
                     DILI"
BASE COUNT         4708 a         1284 c         2032 g         3952 t
ORIGIN      
        1 taggatgtaa aaataaatag gatataaaaa tatcgtaaat gtatgaggag tgaaatttaa
       61 tgcgtttcaa aagaatattg atattgatgt taactgtagc aatgatagct ggaatgttgg
      121 ttgggtgtgc taataaccca agcaataatc ctagctctag caaagataat aaaagtgata
      181 taagcgataa aagtaattca aaaaaatctg tatcaatgat actagatatt gaaggaacaa
      241 ataatgaagc tatgaataat tcagctttat tagcattaaa caatgctcaa aagaaactta
      301 acatagatac aaacaaagtg gaatcagatg attcctcaac tttttctaat agtatagata
      361 ttttatgtaa tgacaattac gatttaatta tagctgtagg agctagattt gcaaaaccat
      421 tggagatggt agccaagaaa tatccaaagc aacaatttgc aattatagac tatgagtatg
      481 acaagcaacc ttctaatata acttcaattt cttatgaaga taataaatct ggatatctag
      541 ctggattaat tgctggaaaa atgactgaaa gcgataaggt tggttttata ggaggaacaa
      601 agagttctag tagagataaa tttgaatcag gttttagaga gggtgttaaa ttttctaata
      661 gtagtataaa ggatatttca gttgagtatg cagatgtttt taaagatagc aaatcagtag
      721 aatctatagc taaaaaaatg atggataatg gagtggatat tattttttct actactgaag
      781 atgattcaaa agctgtaata gatgcagtaa gggcaaaaaa taaaaaagtt atagcaacga
      841 ataaagacca acatgagtta gctccagaaa atgttataag ttctattgta aaagattttg
      901 agaatcctac ttataatctg atacaaagtt ttgtaaaagg aaattataaa ggcggaaaag
      961 ttatagaaaa cacagttaag tctggaagta ctgggcttgc ccaaaatagt tctcaaaata
     1021 ttcctccaga tgtgttagaa tatgttaata agaacaataa ataatctact ataatatcca
     1081 agaatactta tgactttagt tatgaggtga attggatgtt ataatagaca tatatagaaa
     1141 gacatatgca gaggtaggtc agacctatct aaatcaggga gtgaattttg tgaaaataaa
     1201 aaggatattg atggcttgct ttgtagtagt ttttacaatt tttatattag ctatatgtaa
     1261 ttttgatttt ggatttgata aagtaaataa gaataattct aatatagata gtgctgaatc
     1321 gaaacagaat gagtctaaaa gtttgccaac ttcagcagaa aaggataaat caaatagtaa
     1381 aagtaagaat aaagaagatg acacagaaga tgattcatat gtaagaaaaa ctattggagt
     1441 attgttagat accgaaaact taaataacga atcatataat aacaatgtac tatcagcctt
     1501 aaaaagggct gacgaagatt ttgatgttag gataaaactt gaaaaaccaa agacttctaa
     1561 aagttttaaa gagagtatag aaaaactgca taagtatgaa ccagatttga taatagcagt
     1621 tggagccaga tttgcagagc caattaaaga agctgctact aaatatccca aacagcaatt
     1681 tgcaatagtt gactatactt ataaagttca gccagctaat gtaatttctg tatcatttca
     1741 agataatgtt actggatatt tagcaggact tattgcaggt aaaatgactg atactgggaa
     1801 agtcggtttt ataggaggag ttgagggttc tagtagagat aagtatgaat ctggatttag
     1861 aaaggggctt aaaacttcaa ataaagatgc tgagttacta gttaagtatt caaatatatt
     1921 tgaaaaccct gaatcagcta gaactattgc aaaggaaatg agagattctg atagagatgt
     1981 tgtattttca gctacagaag atgatagtaa aagagctatt caggtagcaa gagaaaatgg
     2041 tggtaaagtt atagctatta ataaagacca atacgatatg gctccagata atgtaataag
     2101 ttcaattata aaagatgtag aacaacctgt atatgaactg ataaagaacg ttgcaaaaga
     2161 tggttttaag ggaagtaaag taatggaatt taaaataaaa gatggggaac ttggcttgac
     2221 ttcaaagagc agtagaaata taccaccaga tgttttggaa tacataaaaa aagagtataa
     2281 caaagtgaaa gatacatatt aattattgat gtagaagatt ataaaaatat attgcttaag
     2341 tttagattag caataaaaag atatatttat taaatttgtg ctaactgtac ggaatatact
     2401 gcttaagttt agattagtga taaaaagata tatttattaa atttgaacca actgtacaaa
     2461 gctatactaa ttaagcttag attaactata taaaattata acaataagtg tgtgggcaga
     2521 aaaattctgc tacacacttt atttatattc acataaaaaa tttatcaaaa aaaagaaaaa
     2581 ggagtaaaaa atggaattta caagagaacc atttatacta gatgacgatt tgtgtctagc
     2641 tttggtggac aaaagaatta gttatctaat ggaaaatgaa ttaaaattaa gaaatataga
     2701 gataataaaa acaatagaat gtaaagaatt atatgaaagc ataaaatatc acccggatat
     2761 atgtatttgt aatttaggaa aaggagatat aattgttgcg ccgaatgtat ataatcaata
     2821 caaagagtta ttaagtagat ataaatttaa tataattaag ggagaagcta atttattaaa
     2881 tagataccct tatgatatac cttacaatat tgcgatagtt ggtgactatg ctatacacaa
     2941 ttttaagtat actgacaaag caatattaga ttatattgaa aataataaac ttaaaaagat
     3001 aaatatagag cagggctatt ctaaatgctc aatctgtata gtagatagaa cgtctataat
     3061 aacatcagat aaaggtattt ggaaaagtat gaagaataca gaaatagagt gtttattgat
     3121 agaaaaaggt cacataaatt tatttgagat gaattatgga tttataggtg gatgtacagg
     3181 gttgatttct aaggataaac tagctttttg tggagatgtt aaaaaacatc ctgactatga
     3241 acgtataaaa agttttgtag aaagtaaaaa caaggagata gttacactta gctgtgaaaa
     3301 tttactagat ataggttcta tagttcctct catgacaaga aaggagcgat aactttggtt
     3361 gagaaggata atatttcttc catgaaattt ttgaggaata ataatgtaaa cgatagatta
     3421 atatatacga atgacattgt agaagtagtg atagattctg caaagcaaga agtgttaaat
     3481 aaaaaaatac ttaatggtac taaagctgaa caaagagctt taaatattga tgagatatca
     3541 acggaaataa ctagtatgat aataagtata atgaaagaga aacttttgaa agaatacatt
     3601 ctaaaatgct atgggcgtac atatcctgaa gataaaaata atatatacat atattcactg
     3661 aacatatttg ctaaaaaaga gatttttatg agagaaacag tatttactag ggttagaaat
     3721 tatatagaag aaaatgattt tataaatgta gaaggtttta ttagatttag aatgagagaa
     3781 tttatgaaat atatttctgc tataggtgat attgcagttg aggagtattt aataaagaaa
     3841 gaccaagagg aatttataag agttttaaaa tactttatag atactcaaga agaaaagatt
     3901 gatttgctta aagttcatat aatggaagat aatacatttg ttttatacga taaaaatgga
     3961 aataagatag acagtataga tgatgaagaa attataaata tggtaataag agaaaattta
     4021 aattatgagg actttttaat aagtacctta ttaactctat gtcccaaaaa aatagaaata
     4081 ttggatttgt taaataataa ttgctcaaag gaaattatag atactgtaga agcaattttt
     4141 gaaaataaag ttagtataat tatggaaaac tagaatctaa gcatataaaa gctatccata
     4201 taaaattaaa aaaattcttt ttaaatcgac acaaaagact tataattgta tataaagcta
     4261 tagtataact agtgttatta agtataaaga atatctactt gattaataca atatagctgg
     4321 caaatataca attgataagg agtaatttta taatggataa tttaattagg atactgatat
     4381 tagctgttat tggtggattt ataggttatg ttacgaatgt agttgctata agactgatat
     4441 ttagaccaat agagcctatt aaaataccta ttttaaatat agaaatagta ggtcttatac
     4501 caaagagaag agcagaaata gcagcaaatg taggagaaat aattcaagaa gaatttcttt
     4561 ctatggacga aatacttgca aatatcatta ctgacgaaga taaggaagaa gttgtaagat
     4621 acataaaagc aagggtaaaa attataatac atgagaaagt ttcatttatc ccgagtggta
     4681 taaaaaacat gatacaagat tatctaggag agataattga gtctgaagtt aagcaaagta
     4741 tagatgaatt gagtaaaaat ataataaata aagcaaatga acgtatagat atacaaaaaa
     4801 tggtcgaaga taagattaat gaactagatt tatatgaatt ggaagagata ataataagaa
     4861 tagctaagaa agaattaaaa catatagagt ttttggggct tgtcttaggg tttttaatag
     4921 gtatagctca agggttaatt actatgttta tttaaatata tgagaagtag catttattta
     4981 agtactaatt atatttacct atttgagtaa attacttatt aatagcattt agatattaat
     5041 tgtatttacc tatttgagtg aattacttat taatagcatt tagatattaa ttgtatttat
     5101 ttatttgatt gtgttactta ttaatagtat ttaagtattt actgtactac ttattaacta
     5161 tattaagtat tgattgtatt taagtataca aaacctaaaa agagtacaaa aattagaagt
     5221 tagttttaat aaaaattagt tttagtattt atctagtcat atctatcatg catgtggata
     5281 cgttttaaaa attttaaatt tatgaaaata ttataatatc actataaatc aagagattaa
     5341 tttaatataa aaatataaga atattggttt acagctgatt gagatgaaaa ataatgggta
     5401 attaatatat tacattaatg ttgattgatg tataactatc tgttataatg aaaatatatt
     5461 aaaaatcata gtgttgaaga ggaagagtag agtagaaacc attctcagag aatttgggtc
     5521 tatggctgag agcccatgta ataggtctat ttgaaaacta ccttggagct gagcttatta
     5581 gcagtatttg ttttgtacta tacgtgaaag tgctcgggtg acgaccttat agtcatcaag
     5641 agggttgtat atacttgtat acgccaactt gggtggaacc gcgaattaat atcgtcccaa
     5701 acatatttgg ggcgattttt tattacaaaa aatctaaaaa ttatttcaat aaggaggaaa
     5761 aaacaatgat taaagttgct ttaaaagatg gctcaataaa agaatttgaa aatgctattt
     5821 ctgttatgga tgtagctaaa tccattagtg aaggattagc tagaaatgtg gtagcagcgt
     5881 ctgtgaacgg agaggttgta ggtctggacc acattataga tactgattgt gacttgaatt
     5941 tatttaagtt tgaagataaa gaaggtaagg aagtttttag acatacatca gcacatatcc
     6001 tagctcaagc tataaaaaga ctttacccag aagcaaaact tgctatagga ccaagtatag
     6061 aaaatggatt ctactatgat atagatttag accatagatt agttccagag gacctagaga
     6121 aaatagaagc tgaaatgaaa aaaatagcta aagaagattt aaaaatagaa agatttgaac
     6181 ttcctagaaa tgaagcttta gagcttatga aagaacaagg agaagactat aaggtagaac
     6241 ttatatctga tttacctgaa agtgaaataa tatctttcta caagcaaggt gattttactg
     6301 atttatgtag aggaccacat ttaccatcta ctaaaaaggt aaaagctgtt aaattacaaa
     6361 gtgtagcagg tgcatattgg cgtggtgacg aaaataataa gatgcttcaa agaatatatg
     6421 gaacttcttt tgagaaaaac aaagacttag aagagtactt acatttacta gaagaagcta
     6481 aaaagagaga ccacagaaaa ttaggtaaag aattaggatt atttatgata cctgaagaag
     6541 gtccaggatt ccctatgttt ttaccaaaag gtatggaact taaaaatgaa ttattaaaat
     6601 tctggagaga aatacacaga aaagctggat atattgagat agaatctcca atcatattaa
     6661 atagaaaatt atgggagact tcaggacact ggtatcatta caaagaaaat atgtatacag
     6721 ttaaaataga tgatgaggat tatgctataa aacctatgaa ctgtcctggt ggattaattt
     6781 actataactc tcaactacat tcatatagag atttcccaat gagggttgca gagcttggta
     6841 gagttcatag acatgagtta tctggtgcat tacaaggatt aatgagagtt agagcattta
     6901 ctcaagacga ttcacatata ttcatgttac cagagcaaat taaggacgaa ataaaaggag
     6961 tagctaactt aatagatggt atctacaaaa cttttggatt tgaatataat ttagaattat
     7021 ctacaagacc agagaactct atgggaagcg acgaagagtg ggaagcagca gaaaatggat
     7081 taagagaagc tttagaagag ttaggtctac catatactat taatgagggt gatggagcat
     7141 tctatggtcc taagatagac ttccatctaa aagattgttt aggaagaact tggcaatgtg
     7201 gtactatcca attagatatg caacttccaa gacaatttga taatacttat attggtcaag
     7261 atggtgaaaa gcatagacca gttatgattc atagagttgc ttttggtagt atagaaagat
     7321 ttataggtat attaatagag cattatgctg gtaaattccc agtttggtta tctccaactc
     7381 aagttaagat acttcctata tctgataaat ttatggatta tgcaaatgaa gttaagaaag
     7441 agttatttga taagggtata agagttgaat tagatgatag agctgaaaaa attggattta
     7501 agataagaga agctcagctt gaaaaagtgc catacatgtt aatagtaggt gaaaaagaag
     7561 tagctgataa taatgtttct gttagatcaa gagataaagg tgaaataggt agtattaaat
     7621 tagatgaatt tatagctagt atttcaaaag aaattgaatc aagagaaagt attattcaag
     7681 actaatatac aatatattag tagccaagat attgttatat aagataagtt taatcaatat
     7741 gttgatatgt tgatgcatag tgattaaatt tatgaaaaga tgagtataat tagaggtaca
     7801 ttaatcatat ttttatgatt aatgtacctc tagttttttg tatggtagat ttattaataa
     7861 atatatcata gaagtgtttt gaacatggaa atatttatgt gcattcttta aataaaagtt
     7921 tattttttga gtgataatta agtacttgtt ggtaaggcta tggctgtaaa aatcataact
     7981 ttattttcat atttgttcca ttttcataaa aattattatc cctttaagaa aatttcttaa
     8041 tcctcttgaa atatttcctt tttagtctaa agttaaattt ctttcttcaa aatgaatatt
     8101 taaatgaact aaaatttaag tacactttct aatcttatga gtctattttt catagataat
     8161 tgccctataa tagaaggagc catgttgggg ctatgtggta acaataacag tattgccagt
     8221 gattaattaa tattttgtaa atattaatta atgtcttatg ttatttttgg acttacgccc
     8281 tatacataga ttaatttatt taactttaga tatagagaga atgtcaaata catacacaga
     8341 cgttagagtt taagaaagtg tttatggagc aagaactttt aaaagaggta gtgacttagc
     8401 ttaatttcaa gaaatgataa atacaaaaga ggaaaaagag aggtgttgaa atgatagaaa
     8461 aagtagaaga aaaatctaag attatcaaaa catcaaaaga atataagcga actatagttg
     8521 aagaaaaaga taaagaatgg accaaaaaat taaatgacat acttagtaga agtagttttt
     8581 ctaatgatga attagataaa atcaaggagt taattccaaa agagattttg actagtgaaa
     8641 atgttggaga agttgtgact gaagatggat atgctaagcc cgagtatgat gaggtattta
     8701 aatcattgtt cactttaaat aatgaatatg atttacttgt aaattttata aatgatattc
     8761 taaaagatgc tccatatgct aacaggaata tcaaaccgtt cactcaaata aagagaataa
     8821 ttagagttga aactgaccca acgattaatt acattggaga gaaaagacct agactcgata
     8881 tcttggctga agatgaagaa aataatcata taaatattga gatgcaaaga gtttttgaga
     8941 acgattattt ggaaagagct gaatattatc ttagccgtgt acatggtaga aagttagaag
     9001 aaggtaaaga gtataaagaa attggaaaaa ctattggaat acatatacta aatcatgtaa
     9061 aatataatca catagaagat tatgtaaatt gcctgagatt aacaatggac gggcatccag
     9121 atatttattc tagtaaaact gcattatatt ttatagagct tccaaaaatt cataaatctg
     9181 actatatagt aaatagaatt atgctatggg gaaaacttat tagtaatcct tctagcttag
     9241 atgtaagagt tatagctaag aatgattcta ttattaaaaa agcacttgat agattaaaag
     9301 aattaggtag caatgaagaa tatttattaa atctgaaaag aggtgcttat atcatgaata
     9361 aaagtagaaa ttttaaagaa gagattgaaa gagagacagc aattagagtt gcaaaagaaa
     9421 aagattataa aataattata atgttgaaaa aaaacggttt taaattagat gatatattaa
     9481 ataaatttaa tgattttgac ttaagtgaag atgagataaa agaagtctat gaaaataatt
     9541 gatgtgtaaa taaaaagtga acttatataa gtttgctgaa tctaagtctg gaaatattta
     9601 tgtgtatttt ttaaatgaat gaaaatttat tttaaagacc ataatttaga ggtacagtta
     9661 attatatttt tatgatactg tacttctagt tttttctatt atagatttat tatttattaa
     9721 taaatctaca atagaagtgt ttttgttgta agaatcattt tatctaatga ttgtttttta
     9781 taatagttaa atttccattt gtagtgctta aatataaatc tgtaatacca tttttataag
     9841 tgtataaatc actatgacga ttcaattcaa agtcactgtc caaaacacca gaaactttat
     9901 caaaatttaa agtgaaacct ggattatcag gaatagacaa atcgacattg gcagaagtag
     9961 tttcacagta cagagaagac aacattttgc tagaagtgat ttttatatta ctgctagttg
    10021 actcagcttt aatatcagaa aaaactccag ataagttcat ttttccagaa actgaactac
    10081 aagtaagatt tttattgtat atatctttta aatttatatc accattagtt gtttctaagt
    10141 ttaaaacatc aattttccca gaaagctgta cttcaccaga tgttgattta agtgtagcat
    10201 ttttactatt tagttttgct acatcaatgt gaccactagt tgtgtctaaa tttattttat
    10261 taatattacc agagagacct attgcacctg aaactgtgtt agcttcaact gtattacttc
    10321 ttatgtaagt gctatatata tctgctgaag tagtatataa ggttatttta tcataaagct
    10381 tattgggcaa atatagttca agtattgttg gttttggaag attaattcca atgaagaatt
    10441 ttttagctga attatcatat atagaaagag aatctccatc aaccattatt tttgctagtt
    10501 ttttctttga gaaattacta gggatttttt gaattaattt gattttatta gaatcacttc
    10561 tcttaattct aacttcacct gaaatccaat caacattaat gtcatttatt ttatctgaaa
    10621 aactttcatt tttagccact atataattat cataattgta tttaggatta agaatattaa
    10681 aagggaaatt aaaagaatgt gaaattatag ttcctataag gagtgagaca aatacaatag
    10741 aaagtgatat tgccaggata ctccagatga aaatctttgc ctttataagc tttttattca
    10801 tatttatcct cctccttgta gccttttagt tgtataattc ctttgacaat gttgacacca
    10861 gcagcaccta ttataaatag tatccacgaa aaggaccaaa tattaaatag tatgcttgtg
    10921 attagatata tagaaactat tataatccaa tatgcattca taaatgattt ataaactgga
    10981 tttatatgat tataattttc ctcaaaatcc tcaaattctt tgaatttatt tgataaatta
    11041 tcattgtata gagcatagtt tgtccttgtc atttgattat agactaataa acctactcca
    11101 agtgctatta atatgagcat taaaagaata gctatagttg gttctatgcc aaaacttaca
    11161 actgagacta caaataggaa agggcttatt ataaaaagag caactgctat tgatgctctt
    11221 aaggctcttt tctttttttc ttcaattaat tcagaaggta gagaatatgg ttccttaaca
    11281 ctttcaatta actcagataa atcaccaatt cctgagatta ctgagttata tgcttcttgt
    11341 tctgttttac catcttgtat ttggtcgtta tacttttctt ttagattttc aaataattca
    11401 tctttaagtt ctttgacttt ttttgtctca ttaactcctg taaagctact gtcaatataa
    11461 gtacgtattt tgtctaacat attttcacct cctaaattaa tatatctatc aatgcttttg
    11521 tattattcca ttcttcacgg ctttttatat atttctcttt acctatgttt gttattgaat
    11581 aatactttct tctagctcca gtaacttcat caccccaata agaggttatt gcaccagatt
    11641 gttcaagtct acgaaatgct gtatacaaag tagcttcttt caactcatac aatcctttgg
    11701 ttttttcaag tatagattta ttaatctcat aaccataact gtctttttca agtaaatgtt
    11761 ttaagataat tgtttctgta tgacctctta tcatgtcaga tgcgattatc atgaacatca
    11821 cctccaatta aatatataat actttgatat acttcgcctg taaaggtata tcatgagaaa
    11881 agacatttta ttgtaatatt tataagattt tatatagaaa taatgtaaaa actttataaa
    11941 taatatattt tatacctagt tatagataaa tttata
//
LOCUS       DAEORZ010000127        11850 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEORZ010000127 DAEORZ010000000
VERSION     DAEORZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.128"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..1077
                     /locus_tag="KRH39_002975"
     CDS             <1..1077
                     /locus_tag="KRH39_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG8512862.1"
                     /translation="SKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEITKIF
                     EIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNLDII
                     MSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNGIEP
                     PLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPFIKK
                     INQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGNSIG
                     KSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPMLIV
                     FMGLIIGTFILAMVVPMFDAITNF"
     gene            1131..1490
                     /locus_tag="KRH39_002976"
     CDS             1131..1490
                     /locus_tag="KRH39_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG8512863.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            1543..2415
                     /locus_tag="KRH39_002977"
     CDS             1543..2415
                     /locus_tag="KRH39_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBG8512864.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKGHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            2416..2859
                     /locus_tag="KRH39_002978"
     CDS             2416..2859
                     /locus_tag="KRH39_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512865.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSRHTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            2874..3458
                     /locus_tag="KRH39_002979"
     CDS             2874..3458
                     /locus_tag="KRH39_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512866.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            3711..4748
                     /locus_tag="KRH39_002980"
     CDS             3711..4748
                     /locus_tag="KRH39_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG8512867.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            4829..5194
                     /locus_tag="KRH39_002981"
     CDS             4829..5194
                     /locus_tag="KRH39_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512868.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            5199..5516
                     /locus_tag="KRH39_002982"
     CDS             5199..5516
                     /locus_tag="KRH39_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512869.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            5564..7168
                     /locus_tag="KRH39_002983"
     CDS             5564..7168
                     /locus_tag="KRH39_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512870.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            7292..8920
                     /locus_tag="KRH39_002984"
     CDS             7292..8920
                     /locus_tag="KRH39_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG8512871.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            9185..10534
                     /locus_tag="KRH39_002985"
     CDS             9185..10534
                     /locus_tag="KRH39_002985"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBG8512872.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDSSKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            10690..11769
                     /locus_tag="KRH39_002986"
     CDS             10690..11769
                     /locus_tag="KRH39_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG8512873.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKTGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
BASE COUNT         4949 a         1152 c         2050 g         3699 t
ORIGIN      
        1 tctaaaaata tattttataa tttagaaaag ttttatttag tattaaatag aatcagtgat
       61 aaagaaatta gagtaatttg caaagagata ggcatattgc tggaatctgg atgtgaaatt
      121 acaaaaatat ttgaaatcat tgagagtcag tcaagtaaaa aagtaaagaa cttgcttagt
      181 atagtatcaa atcatattca aaaaggtaac tcaatagcag aatcatttca aataacaggt
      241 atattttcta aattctttat aagtatgata aaagcaggag aaactagtgg aaacttagat
      301 ataataatga gtgatttatc taattattat gataaagaat acaaattaaa aatgaaaata
      361 ataactattt caatatatcc aataatactt ataatattat ctatcttatc aatgttgttt
      421 atattcgtat ttgtaatacc taattttcag gtggttttta ccaacaatgg aattgaacca
      481 cctttaataa ctagagtttt aatgggaata tctacagtgg ttacaaataa cttagcttat
      541 ataatattta gttttatatt gttttcaata ggaagcattt atttctttat aacaaatgat
      601 aatataaaaa aattgattaa ttcgttaaaa ttcaaaatac cttttataaa aaaaattaat
      661 cagttagtag ctacaacaag attttcaagg gcattgtccg ttttaattag cagtggaata
      721 caaattgtgg aagctataga tatgtcagct agtgttatag acaacgaatt tatttatgaa
      781 aggttaaaga tttccaataa ttatataaaa aaaggaaata gcataggtaa atctttgaat
      841 ttagcaaata tttttccaaa attatttatt tcaatggcaa atacaggaga agaaagtggg
      901 aaactagata aaagtctaaa tgtaataaat aaattttatg aaaatgaatt aaataacaaa
      961 atagagcaaa taatgaaatt tatagaacct atgctaattg tatttatggg attaattatt
     1021 ggtactttta ttttagcaat ggttgttcct atgtttgatg caattactaa tttttagaga
     1081 gaaaaataaa ttttattaga tgaaactttt gaaaaggaga gaatatttaa ttgataaatt
     1141 tgataaataa aaaacgaaaa ggatttacac ttgttgaaat gattgtagta gtaactattt
     1201 taggcgttat atctagtata gcattagtta agtatagtaa ggttcaagaa agtgccaaat
     1261 taaatgcaga ctatacgaat gctgctaata tagtaaccgc agctagcatg gcaattaatg
     1321 atgatgaaaa gacaatagac tctctaagtg tagagacatt gaaggaaaag ggatacctaa
     1381 atactgttcc agttcctcag agtacatcag gtaaattcga acttgtcata aatgatagcg
     1441 gaacagatat aagcgtaaat ataaattcta agcaatttta tccaaaataa tattttatat
     1501 tgattgctat aagttttaaa attattttta tttaagaagg agatggctat aattaaaaaa
     1561 attctaaact tagaaataaa tgatgattat ataaggcttg tatttttaag aaaaggtcat
     1621 aaaaaagttt ttatagataa atcaattata aaagagatta actttgatat aaaaaatgat
     1681 agtaattcga ttgagaagat taaatatata gaagatgtag ttagatttgt aaaagaggag
     1741 attggtaaat caaaaatatt atataaaaat ttatacttta acattcaaat tcaggatata
     1801 gtcattagaa atgttgaagt tctaaatact aagaaaaaaa gagacatatt aagcatgatt
     1861 gaatttgaaa taacacaata cattccaata aacataaata actattcaat aaaatataaa
     1921 gttataaatt ctaaaaatga taaattgaat atacaagtaa ttcttttccc aaagagtatt
     1981 attcaattaa ttaagaacat ttcagaaaat ctaaatatga atccaaagac tataaatata
     2041 aattttgata tattacaaaa gcttataaac ttaaatttaa tagagaattt ttcagaagaa
     2101 ggggtattta tagaatgtaa aaaaagtcag ttaattataa atataacaaa agataaaaaa
     2161 ataagagaaa catacttgct atcaaaagag ggaaaatcat atgagttcat aaaaagatta
     2221 ttaagggatt ttaagtatgt ttattactat ggtatagaaa attgttttat tgaagaatac
     2281 tttaaatata cagataaatc tattgaagta aatccattaa gattaaaaga taaagtaaaa
     2341 gtagaaaata attttgatgc agatttagaa gacaatagga taaattatat aaatagtata
     2401 ggaatgatta tttaagtgaa agtggataaa acaataaatt ttcttaatgg atttaaaaaa
     2461 actaaaccat gtaataaaat ctataaaata ataatagtct taattttaac gtgtatttta
     2521 tttaaatcat ttatgtatgt gaaatctata aatgaacttg aagattatat aagtcaaaat
     2581 aagctagtgg ataaatcaag acacacttta ccaacaacag ataaaactag agagaaattt
     2641 tctaaaataa aacttaatga aataaaaaaa atatatgaaa caattggaat aaatagtatt
     2701 actaaaatat caatagataa aaataagcta gagatggaag gtgtgtgtag tgatttagat
     2761 gttttaaaca atttaaggaa aataaagtac ataaaatatt attacataga cagtattgta
     2821 agagatggag aatattatag atttagaata aaaagttaga tgggtgatta tttttgagat
     2881 taaataatag agaaaaaaat ctacttaaaa tattgttaat attagttatt atattggcaa
     2941 gtgatatagc agtaagttat ttggaagaaa acctaactag tttgaagaat gaaaagcaaa
     3001 atataataaa taaaataaat aatcaagata attatcaaat aaaggataat aagcatatag
     3061 atgataaata tatagaaagt gttgaatcaa ataaaaatga agaattaaaa aataatttta
     3121 ataactcatt tttagataaa aaaacaaata aagatatagt gttattagta gaaaatgaac
     3181 tgagagatat tgtgaatgta gaatatatta atcaagaaag tagtttagat aatcacaata
     3241 aaaaacaagt taatttagaa ataaaagtta atggagaact tgcaaatgtg ttgaaacttg
     3301 aaggtgtaat gaagaagtta agactaaata ataaacttga aaagatagaa attaataaat
     3361 taaaagtcaa taattttgat gttcaaaata ttccaaatga atataaacaa gaaaaagaca
     3421 atattgttga atgtaaaatg acagttagag tcatataaca aaaaagaata gataaagtaa
     3481 aaaactatta tcaattaata aaattttata gaggaattag ttttacgata attcatttct
     3541 acaagaggga ttgttgatga ttttatcgaa atcctaatta aagttaatat attgtaccaa
     3601 aatataagtg aatttaatag aatatttact tatactatag attgattaat aaaaaaatgg
     3661 tataataaca aattgtactt taatttaata ataagggagt ggaagtcata ttgttaggga
     3721 gaaatgagct atgcccttgt ggaagtggca aaaaatacaa gaaatgttgt ttaaataaag
     3781 atattgtttc agaaagagca tgcagaaaaa ctgaactttc acaaaaacaa tatacagatt
     3841 tatattcaaa gttatatgat tattcaagac aggataagtt taaagaagaa tgtgaaaaag
     3901 ctaaagaaat gttttatata atgcaaaatg atagtgtaaa tgaaaaattt gaaagatttt
     3961 ttaatactta tttaatacaa gaccacataa tggaaaataa aaaggttatg acagttggtt
     4021 ttcttgaaga aaatggtgca aacctaagtc aatcagaagt atcaatatta aaaagtatgt
     4081 ttgaatcata cgtaagtatt tatgaggtaa aagaagtttc tgctggaaaa atagtattaa
     4141 aggactgttt aagtggaaat gaactttgta cagaagatgt aaaacttctg agaagtttta
     4201 aaatcggaag ttgtatgata gctagaatag ttgatattga aggaactaac atattgatag
     4261 atataactat aagtatatca aatgaggtta aagatataat tttaaaggat attatgaatc
     4321 tattcaatca atatcaagat gtatacaaag atatgaagtc gttcttaatt tatcatacac
     4381 atatactata caagtatata caacagcttt tagacccaag tatagctgaa tatttaaaga
     4441 gagaaagaga taaaaacgat acaaaagtta attcagaaaa tgaattagct gaagaagaat
     4501 gtaaagtttt agatactctt aaagaaaatg tagaaaaaga aaatatagat gcatgtatag
     4561 agttttggaa tgaatataag aaaaatcatg aaaatataaa aggagctgaa aatggttggg
     4621 cagctgctgt tgaatactat gttaaaaaag aagcaggaca acctataact caagttcaaa
     4681 tatcaaagaa atacgatata agctctagta cactaggaaa gagatacaag gatttaaaaa
     4741 tatcttaatt aattataaaa agctttaagt catagataaa gaatctatag tataatatat
     4801 gtataatgtt tagaaagagg tattaataat gagtcttagt ttaggaaatg caattgctgt
     4861 atcaacgaac tttataaaat ctaacccagc tgtacctgtg tacaacggag aagaaatata
     4921 tgcagttatt tatcatatag aaataggata taataatggt gtatcacctg ataccaaaca
     4981 atatataata atagactata aagagtttga tttatctatg gataaaaaaa atgaactaat
     5041 aaatattgct aaagagtatt gtaaatctaa aggtattgta gaaaacgaaa accttgaaat
     5101 aaacctttta gacaatgaag aagcagagaa cttcttagaa aaggtgttta ataatatgaa
     5161 ggttataaga ggtcttataa ctaaaaaagg ataaataaat ggactatata ggaatagaaa
     5221 atataacacc ttatgaaaat acatatgaat ttagtgtata tgaatatgat gatgaaatca
     5281 ccttaggtag tgaaaagtta tatgtatgtg aattaagggt tgtattgatt aaagttaatt
     5341 ctctgtatgt tgaaagattg cataaatcag ttgaagcaat ggtcttagta aaaaacttga
     5401 aaaaagattt agataaaaca cttgttgtaa acaaaataaa gaattttgtg ctagatgaga
     5461 tttgggtaga aaatctagta aaagagaata tagaagttat atttgtagaa agctaggaat
     5521 aaccctagct tttttatatt acaatctaag agaggaaaac aatatgttaa gagtatctaa
     5581 tgtaaaactt ggaattgata aagatatatc tttattaaaa gatgttatac ttaagaagct
     5641 aaaaataaaa gaaaaaaact tagtcaaata tagtatatat aaagaatcta tagatgctag
     5701 aaaaaaagga aaaatggaat ttgtttattc tgtagatgta gaagttaaag atgaaaataa
     5761 aatacttaac tcaaaaataa aagatgtaac aaaaataaag gaaatcagat acgtaaatgt
     5821 acctgtgggt gataaaaaat taaagaataa tcctttggta attggtagtg gaccagctgg
     5881 tttgtttgca ggtctgcttt tagctcaaat gggttatgaa ccaatcatac ttgaaagagg
     5941 tcttgatgta gacagtagaa ccaaagatat aagtgatttt tggacaacta gaaaattaaa
     6001 aaataattca aacgttcaat ttggagaagg tggagcagga actttttcgg atggtaaact
     6061 tacaactaga ataaaagata tacgatgtag aaaagtatta gaagagttag ttcagtttgg
     6121 agcaccagat gaaatattat actcacataa gccacatgtt ggaacagata tattaaaaaa
     6181 tgtagttaaa aatataagaa atgaaataat aagtttaggt ggtcaagtta gatttaattc
     6241 aaaagtaaca gatatagttg tatcaaatga ttctatacaa tctgtaataa taaatgatac
     6301 tgaaaaaata gatacagata caattatatt agctgttgga catagtgcaa gagatactta
     6361 tgaaatgttg tattcaagag gtataaaaat agttcaaaaa ccttttgcaa taggagctag
     6421 agttgaacat ccacaagatt taataaataa agcacagtac aaggattttt ataatcatcc
     6481 taggttagga gctgcagatt atagacttat agagcatgtt tcaaatggaa gaacagcata
     6541 tactttttgt atgtgtccag gaggtagtgt catagcatct gcatcaaatg attttgaaat
     6601 agttacaaat ggtatgagtg aacatgcaag agataaaata aatgcaaata gtgcattttt
     6661 agtaaatgta acgccagaag attttaagag tgataatcca ctagcaggtg ttcatttcca
     6721 gcagaagtat gaaaaattag cttttgaatt aggtggaaaa aattataatg caccagttca
     6781 gcttgttgga gattttataa acaataagac ttctttgaat ttaggaagtg ttgagccatc
     6841 atatagacca ggatatacac ttacaaattt aagtgaatgt ttgcctagct tcgtaacaga
     6901 gactatgaga gaagcgttta tatctcttga taaaaaattg aatgggtttg caatgtatga
     6961 tgctgttttg acgggagtag aaactaggtc atcatcacca ataagaatag taagagatga
     7021 aaatacttta gaatcagtga gtattaagaa tctatatcca tgtggtgaag gtgctggata
     7081 tgcaggtggt atagttacag cagcagttga tggtataaaa tgtgcagaaa aaataataca
     7141 aaaatacacc aataatacaa aaatttaaca taaacttaat gattttttaa tatttaacat
     7201 gaatttaaca ttatcactcc tataatttga taaaatacaa taggttataa aatttttaga
     7261 tacagtaatc tctttacaag gaggaaataa tttgaacatt ataataagtc taatgggtgg
     7321 attaggtctg ttcttatatg gtatgaacct tatgggagaa ggtcttcaaa aatctgctgg
     7381 gacaaagcta aaaaagataa taaaactact tactagtaac ctatttatgg gagttttggt
     7441 aggtacagga gttacagcag taatacaaag ttcaagtgct actactgtaa tggttgttgg
     7501 tttcgtaaat gcaggaataa tgactttaaa acaggcaata ggagttataa tgggtgctaa
     7561 tatagggact actgttacag cccaattggt atctttcgac ttaactggta tggccccagt
     7621 agcattaggt gtaggtatta tactttattt atttggaaat aagcctagaa taaaaaatat
     7681 agccgaaata cttataggtt ttggtatatt atttacaggt atggatttta tgaagatggc
     7741 agtggagcca ctaagagact atcaaggatt tactgactta cttgttacat ttggtagata
     7801 tccactactt ggattattat tgggatttgg gattacagca ataatacaaa gttcaagtgc
     7861 atctatgggt atgttggttg cactggcagc agaaggtcta gtgccattat cagcagcact
     7921 gccaattctt tatggccaaa atattggtac atgtgtaact tcactgttat caagtattgg
     7981 tgctaacaaa aatgcacgaa gagcagctat gatgcatcta atcttcaatg ttttaggaac
     8041 agtgatattc ttaatattct tgaataaacc agtagtaagc atggttacaa gttgggaccc
     8101 aagcaatgtt gcaagacaaa tagctaatac acatacattg tttaatataa tatctgtatt
     8161 gatactttta ccatttacaa atcttattat aaaactagct ataaagcttg ttcctgatag
     8221 agcaggtgat atagatgaag atgaaactaa aactattaaa tatatagatg atagaatgat
     8281 agaaactcct tctatagctc ttgcaaatac gataaaagaa gcacttcgta tgggtgagaa
     8341 ggcgaaggaa agcctaaatg catctatgga agctttagta gaacattcaa ctgaaaaaat
     8401 agataagaca tataggcgag agagattaat caatgattta cagaaagcta ttttaaatta
     8461 tttacttaaa ctttcaaaag cacctttaga tgatgattca agagaagttg tggatacttt
     8521 gtttaataca gtcaatgata tagaaagaat tggtgaccac gctaaaaata tagctgaatt
     8581 atcgcaagtt gcaatagatt caaatatttc tttctcagaa gaaggacaat ctgaacttga
     8641 tgttatgtat aatagagttg tttcagcata tacttatgca ttagaatcaa tgagaactga
     8701 taatgttgat ttagcttgta aggttataaa aattgaagaa caagttgata taatggagaa
     8761 atcttgtaga gctaatcaca tgtatagatt gaacaataat ctatgtagta tagaaaatgg
     8821 agttatttat ttagacgtaa taagtaattt agagagaata tctgaccatg ctgtcaatat
     8881 agcacaacaa gttatagcga agagattagg aaatgattaa aaataattaa tttaaaataa
     8941 tataaaaatt aaaatagagg ataaatacaa gagaaacttt aaatgataca tttagagttt
     9001 ctttttttgt aaatttattt gactaaaatt taaaaattta tacatagtta tagtatttag
     9061 aagaatcgat ttcctggtaa taaatgatga gatatgtatg attttgtggc aaacttttaa
     9121 aaaaaacacc tatattttgt gttataatct ctttaaaacg aatatgctga aaagagggat
     9181 taaaatggga aaaataaatt ttgattatag taaagctaca gagttttttt gtcaaaacga
     9241 aatagatgtc atgcaaccat atgtagatgt tgcacatgac atgttacata ataaaactgg
     9301 tttaggaaat acttttttag gatggataga ccttcctaaa aattatgata aagaagagtt
     9361 tgacagaata aaaaaatctg ctgagaagat aaaatcagac tcagatgttc ttttagtaat
     9421 aggtatagga ggctcttatt taggttcaag agctgctata gatatggtaa gtcattcttt
     9481 tagaaatgga cttaaaaaag agcaaagaaa ggctccagaa gtatattttg taggtcataa
     9541 tataagctca acatatatta tggatttatt agatataatt gaaggaaagg atatttctgt
     9601 aaatgtaata tctaaatcag gaactactac agaaccagct ctggctttta gaatatttaa
     9661 agattactta gagaaaaagt atggtaagga agaagcaaga aagagaattt atgcaacaac
     9721 agattcaagt aaaggtgcac ttagacagtt agctacagaa gaaggatatg agacttttgt
     9781 aataccagat gatgtaggtg gtcgtttttc tgtattgaca gcagttggat tacttcctat
     9841 tgctgctgct ggacttgata ttgatgctat gatgaaaggt gcaaatgatg ctagagaagc
     9901 atttcaaaat cctgatttaa aaagtaatga tagttataga tatgctgttg ctagaacaat
     9961 tttacataga aaaggaaaag atgtagagtt attggttaac tatgaacctc aactacatta
    10021 tgtaagtgag tggtggaaac agctttatgg agaaagtgaa ggaaaagaaa ataaaggttt
    10081 attccctgct tctgtagatt tctcaacaga tttacattct atgggtcaat acatacaaga
    10141 tggaaaaaga cttttatttg agacagtact taatgttgaa aactgtaaga gaaatataac
    10201 tataagttca gaagaagtag acttagatgg tttaaattat ttagcaggta agactgtaga
    10261 ttttgtaaat cataaagctt ttgaaggaac tctattggct catacagatg gaaaagtacc
    10321 taatttagta ataaatatac ctcaactaga cgagtataat tttggatatt tagtgtattt
    10381 ctttgaaaag gcttgtggaa taagtggata tttattggga gttaatccat ttgaccaacc
    10441 aggagtagaa gcttacaaga aaaatatgtt tgctttacta ggcaaaccag gttatgaaaa
    10501 agaaaaggaa gagctagaaa aaagacttaa ataattttaa atagttaagg tttaattaag
    10561 attaaaataa tataatgaaa tcatcaaaaa ggagtttact caaaatagat gatttcattt
    10621 tttttatgaa aaattgggaa aaatatattt tgataattta tattatatta agatagggag
    10681 tggtatgaga tgtcaagaag aaagaaaggg ataagtttag taatattagt tgctataata
    10741 agttcaatac ttagtagttt tctaacaata attttagtta aggataattt agtaagcaaa
    10801 tctacaggaa gttctacgcc gatagttgta aatgatgatg gtaagagtca aaatatttat
    10861 caagcagttg cagaaaaagc tacgccatct gttgtaggta ttacaacaac tagtgttgat
    10921 actagtaata tgtttgcaat accaactgaa acacaaggtg taggaactgg aatcatagtt
    10981 gattcaaatg gatatatact tactaattct catgttatat cagatggtca agctacaagt
    11041 gtaaatgtat tattcaatga tggttcaaca acttcaggta aagtagtatg gtttgaccaa
    11101 caacttgatt tagctatagt taaggttgat aaaactggac taacaccagc agaatttgct
    11161 gatagtgaca aagtaaaagt tggagatata tcaatagcaa taggtaatcc tcttggatta
    11221 gattttcaaa aaacagttac tcaagggata ataagtggat tagatagaac tatacaaaca
    11281 gaaaaaacta atatgactgg tttattacaa actgatgcaa gtataaatgc aggaaatagt
    11341 ggaggaccat tgttaaatca aaaaggtcaa gtaattggta taaacacagc aaaagcttct
    11401 caagcagaag gattaggatt tgcaatacca atcaatacag ctaagagtat agttgaagaa
    11461 gtaatcaaaa ctggaaaata tgaaaaagta actttgggta taaaaggtac agatgtatca
    11521 aattatgaag cagcaacagg tacaaaatta agtactgata aaggtgttta tgttgcagaa
    11581 gtaatatctg gttcatcagc agaaaaagct ggagtaaagg taggagatat tataactaaa
    11641 gtaggagaca cagatataac aggtatgaat gatttaaata aaaagttgta tactttctca
    11701 aaaggtgcta gtacaaaaat aacagtaaat agaggcggaa aagcagtaac aataaatgtg
    11761 aatttctaaa tatgaccaga aagatggaga ctacatggta aagtgtagtc tctattttta
    11821 tttaattttt atagtatatg catctaaatt
//
LOCUS       DAEORZ010000128        11781 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEORZ010000128 DAEORZ010000000
VERSION     DAEORZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.129"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     repeat_region   71..1354
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=71..99
                     /rpt_unit_seq="ctttacataccacttagttaatataaaac"
     gene            complement(2310..3737)
                     /locus_tag="KRH39_002987"
     CDS             complement(2310..3737)
                     /locus_tag="KRH39_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HBG8512874.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGESSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKIVKDTNSTR
                     LGGTDRYDTNKKTINKFYSGVKEFYIASGTDLVYALVGSTVAKNNAIVLVDNDSNKSV
                     LKSTTKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKIYN"
     gene            3928..4749
                     /locus_tag="KRH39_002988"
     CDS             3928..4749
                     /locus_tag="KRH39_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG8512875.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            4874..6391
                     /locus_tag="KRH39_002989"
     CDS             4874..6391
                     /locus_tag="KRH39_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG8512876.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            6414..6698
                     /locus_tag="KRH39_002990"
     CDS             6414..6698
                     /locus_tag="KRH39_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8512877.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(6795..8459)
                     /locus_tag="KRH39_002991"
     CDS             complement(6795..8459)
                     /locus_tag="KRH39_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBG8512878.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(8531..9778)
                     /locus_tag="KRH39_002992"
     CDS             complement(8531..9778)
                     /locus_tag="KRH39_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG8512879.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            complement(9765..10439)
                     /locus_tag="KRH39_002993"
     CDS             complement(9765..10439)
                     /locus_tag="KRH39_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBG8512880.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKNLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            complement(10611..11690)
                     /gene="mltG"
                     /locus_tag="KRH39_002994"
     CDS             complement(10611..11690)
                     /gene="mltG"
                     /locus_tag="KRH39_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBG8512881.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
BASE COUNT         4049 a         1908 c         1370 g         4454 t
ORIGIN      
        1 tgataattaa atttttactg atatggctat gcttttaaaa gtcctaacct tattttgaca
       61 ttatatctgt ctttacatac cacttagtta atataaaact tgctatttat tatcatccat
      121 acttcctcac atttctttac ataccactta gttaatataa aacttttatg cagcaatata
      181 acgctaaata atttttaaaa gtctttacat acctcttagt taatataaaa cctttattat
      241 tagtagtttt actatcagtt ttattaactt tacataccac ttagttaata taaaacaccc
      301 aataggattg gtgggttacg ctcccacaac gtctttacat accacttagt taatataaaa
      361 catgatagaa ttgtcagcta ctctgacatg gaaggttatg ctttacatac cacttagtta
      421 atataaaact cactaaacga cttacttctt aagctgctaa gacaaggctt tacataccac
      481 ttagttaata taaaacattt gttccaatta gtttgtttac tgctgttact tatctttaca
      541 taccacttag ttaatataaa acgtggctta ataagaaagg atataaaaga gttggaaacc
      601 tttacatacc acttagttaa tataaaacgg tcactacaac ttatacttcc agtctaacga
      661 tttgacttta cataccactt agttaatata aaaccattta actaactgta tttctatact
      721 tttttataat gctttacata ccacttagtt aatataaaac tctatatttg ccgtccgtac
      781 ctttcgttct atatcctact ttacatacca cttagttaat ataaaacaaa tatctctagc
      841 aacatgaaga gattttccac tcccctttac ataccactta gttaatataa aactacatga
      901 ttttctttag cataattaac aaaaaaatcc tttacatacc acttagttaa tataaaacaa
      961 tacctaattc attcatacaa tattcgtgca atgtctttac ataccactta gttaatataa
     1021 aacttgagtt catgctatgc taccaaatag ggttagttgc ctttacatac cacttagtta
     1081 atataaaaca tacaagagac taagacagta aaaattgctg ctttggtctt tacataccac
     1141 ttagttaata taaaaccctt ataaaactct gtccatttat ctcgccaatt cggctttaca
     1201 taccacttag ttaatataaa acaatactat tttcttttat gttgccgatt tcccgacctc
     1261 tttacatacc acttagttaa tataaaactt gaaacaagct aataaattat aaggaggtaa
     1321 taacacttta cataccactt agttaatata aaaccccaaa ataaattgag tatttccaac
     1381 acttacatct atacaattct ctcaaatttg cagtgaacca cgagtagtgc aaatgataat
     1441 atttatcata taccctcaat gccttatgtt tcaactgtta agccctattt tatcaaaaat
     1501 atcgctcact gcaaaactat ttataatatt atgaatatat tatttttaaa tatctgtacc
     1561 aatttgtagt acaataaaat caagaaaata atttactcct ataaagagtg attattttta
     1621 agtgaaaata ttattttttt aaatcattct tattggcgtt tgggagattt ttattttatc
     1681 ataaatataa actgatataa caccagctga catgcttgcc actttagatt attattcttg
     1741 ctacaattat tataacatat aaaacttact tatttttacc aaatatttta tgtaaacaat
     1801 aaatattaac ttatgaaata tctgctcatt tacagatatt taataagtta atagctctat
     1861 aataaaattt ttatcattgt ttataaaaaa ttttactttg cattttttta taaacaacag
     1921 ttttatttat aatgttttgt ggtataataa aagcaagaag aactacaatc tattttgcgt
     1981 tagagtggag ttcatcataa acagagttta ttttttggat ttaaatataa atttaaattc
     2041 aactcgtaag tcactctttg tgcgagagtg gctttttgct tttttgaata gtttactgat
     2101 taagtaaact accacactag ctgttaaact agctaaaaca ttaaacaaaa agttatccat
     2161 gaactcacct cccttcgaat cgttgggagg ataatctttt gtacatgaac tccactctat
     2221 agattgtaag ttaaatcttc ttgctaaaaa tattataaca tattatggta aattatataa
     2281 ttaaaaacac ttgtttaaaa gttctaattt taattataaa ttttcccagt ttctatatct
     2341 ataaaaaacc aaccaaaagt accattacta ttttttgaat tgattttaac agcatagaca
     2401 ttaccactat atatatcatc tctaaaatct atcatatcaa ctactaagtt attgatactg
     2461 actttatgtc tcttagcaca ttcttttaat gctatctgtt ttgcttgttc ctctgttata
     2521 cttcctttat ttttataatc atctggatta catactttca tattaccatt aacatcatat
     2581 gtataaactt tgctcatatc atttttagct acacagtatc taacatcacc ctctgcaccc
     2641 tgtttaatgt aagcaaatac atagtactct tttcctgaaa aatcaaaatt tttatctagt
     2701 agaaatatta aatcttcatc ttctgaataa ttaggttcgt aataaaattt cttcacacct
     2761 aatagttttt tcactgcatt agcagcatct tctactgtat attctcttcc agtattagaa
     2821 tcgcctatat tttttgttac atttaaacac tgctgtaata tactatcact caaatttcct
     2881 atagcagtta atttagttgt acttttcaat acacttttat tactatcatt atcaactaac
     2941 acaatagcat tatttttagc aacagtagaa ccaacaagag catacactaa atcagtacca
     3001 cttgctatat aaaattcttt tactccacta taaaatttat ttattgtctt tttgttagta
     3061 tcatatctat ctgtcccacc taatctagtt gaattagtat ccttaactat tttgtcattc
     3121 attgatgaag taccacctat tacataactt tctacaccag ttgtattaaa tggaacactt
     3181 tttccatcag ttaatactat agctgcttta tctctaactg ctacaggtgc agcactcatt
     3241 gcatcaggct ctcctttaaa agcattagtc aatataactt tatttacttt gttaattgag
     3301 tttatttctt ttgcgacatt atagcttgtc ttaattctat cactaccttg aagtctttta
     3361 atctctattc ctttgccttt tagaacagtt tcagtatatt tatcaataga gctttctcct
     3421 ccaattatat atactttttt tgccttttct aatcttttaa gtgtagcatt tggtatgtta
     3481 tttttcttag tcaaaagaat tggagcgttt atagctcctg caagtccact tgcacttaat
     3541 ccatcagcca ttgtactgtc tgcattaatt aatatagctg tagtatagtt ttgtttgtct
     3601 gcaattatac cagcagtttc gtatttatct gcacctttaa ttgattcaat tttatcaaga
     3661 gcatttgcag atatagggca actagcaata aatatagata aagataaccc tattgttaat
     3721 aattttttaa ttttcataat atctccttct cttaaatatt ttcacctaaa ttatataata
     3781 cttttcagga aaatgttacc ataaatttaa aaagttatta ttacttaata taatattaat
     3841 gaaaaatatc actcttaaat taatgacttt ggtagaatat ggtgataaaa taaaattaat
     3901 gagagacaat ataaggggga ttatattatg ataatcagta aaaaaataac agcaatatta
     3961 gtatcatttt taattatgat atcaatggtt ggatgtgctc caaaagaaag tgctacagaa
     4021 gtagctaaaa agtaccttga tagtatagaa atcaaagact acgaagtagc atataattta
     4081 ttaagtaaag acagtaagga aagtgtcagt ctagaaaaat ttgaagaata tcaagatact
     4141 ttatttaaac ttcaaaccct aaaagattat aaacttggtg aagaaaataa agtagagaaa
     4201 tacaaatatt ctaataaaga gtacacaaat cttgttaaaa taaaagaaac tgatactata
     4261 aagtatatca ctgaaaataa agaagatact gcaaattttg atagatattt tataatcgaa
     4321 gataatgaat ataaattatt atggcataaa gactttaaaa gagctcttag ctctaattat
     4381 gtcaaaatcg ctaggtcaga aacagaaaaa gatgaagata aggattataa taaagctgct
     4441 attgatatca aaaaagctat agaaattgat gataaaaatc cacttgcata ttatacacaa
     4501 tcatatattt atatagagtt acaacgatat gatgaggctt taaagtcaat tgataaatac
     4561 ttagaatatc tagataaaaa taatgaagct tataaaatag acgctagtga tgcttacaat
     4621 ttaagaggtg ttatttttat gagtaaatca gaatttaata aagctagaga atcttacaat
     4681 aaggcgcttg aacttaaccc ggataatgaa tatgcaaaaa ctaatctaaa tgatattaag
     4741 ggattataag gcattaatat tatagaaaat aagtatgttg aaagttaaat taacttttga
     4801 catgcttatt ttctatattt taaactctat tgatttacta tataatagtg cataaggagt
     4861 gtgattactt attatgagtg ttgccatata cttaagaaaa tcccgtgctg atgaagaggc
     4921 agaaaaacaa ggcgaatttg aaactttaag tagacataaa tcaaccctac taaaactagc
     4981 caaagaacaa aacttagatg taattgagat aaaagaagaa ttagtttctg gtgaaagtat
     5041 aatacataga cccaaaatgc ttgaacttct aaaagaagtt gaagaaaata aatatgatgc
     5101 agtcttagta atggacttag atagacttgg tcgtggagac atgaaagacc aaggtatcat
     5161 attagaaaca ttcaaagaaa gtaagaccaa aattatcaca cctagaaaaa catacgacct
     5221 aacagatgaa tttgatgagg agtattcaga atttgaagca tttatggcaa gaaaagaatt
     5281 aaaacttata tcaagaagga tgcaaagagg tagaataaaa tcagttgaag aagggaattt
     5341 cataggaacc tctgctccat ttggatatga tgctgtaaca actggtagaa aagaaagaat
     5401 acttgtacca aataaagatg ctgatgtggt taggactatc tttgacttat atattaatga
     5461 agatatgggt tgtagtaaaa tatcaaaata cctaaataac ttaggcatta aaactgctac
     5521 aggtgctaat tggtacaatt ctgcaattac aaatataata aaaaataagg tatattgtgg
     5581 ctacattcag tggcaaaaaa aggactacaa aaaatctaaa aatccaaata aaataaaaac
     5641 agttaaacta agacctaaag atgaatggat tgaggcaaaa ggaaagcatg aacctttaat
     5701 aagtgaaata acatggaaaa aagctcaaaa tatactcaaa aaaaatggtc atgtcagcta
     5761 tggtaatcaa ataaaaaatc cacttgcagg aatagtgatt tgcaaaaatt gtggtagacc
     5821 attagtatat agaccatatg cagaccacga ctatataatt tgttatcatc ctggttgcaa
     5881 taaaagttct cgatttgaat ttattgaagc tgccatttta aaatctttgg aagacactgt
     5941 aaaaaaatat caattaaaag catctgatat agacttagat aaaaataata aagatagtaa
     6001 tatagaattt caaaaacgag tcttaaaggg cttagaaact gagttaaaag aattaggcaa
     6061 gcaaaaaaat aagttatatg atttactaga acgaggaata tatgatgaag atacatttat
     6121 tgaaaggtcc aacaatataa gttcaagaac tgaagaaatt aaagattcaa ttaagacagt
     6181 aaaaaataga ttgagtactg ttaaaaaaga caattctaaa ataatagaag atattaaaac
     6241 tgtgttatca ctttaccatg attcagattc ccttggaaaa aataaactcc taaaatctgt
     6301 aattgataaa gcagtttatt ataagtctaa agagcaaaaa ctagattctt ttgaattaat
     6361 ggtccatttg aaattacatg aagaccagta ataccaataa catataaaga gtagtgtgta
     6421 atgaaataag tcttttagat atacttggta cagaggctaa ctatgttctt gatgaagttg
     6481 aactaaaagt tcaagttgga aaactttacg aacaactaaa caaaattctt actcctagag
     6541 aaagagaaat tgtccagtta agatatgggt taactcctta tggctataag acacaaagag
     6601 aaattgcaca aaaactcgat atatcaaggt catatgtatc tagaatcgaa aaaaaagctc
     6661 ttaaaaaact agaaaaagag cttgttcaag aaagttaata ctatatttta aaaggatata
     6721 tcgtacatca ttagacaaaa ttttctatct aaaatcaaag ttacgatata tccttaaatt
     6781 ttatagtaca taatttattt tatactttca catatttcct taaatatagg tgttgcagat
     6841 ttatttcctt tttgagttcc ttctactaac acagttatta catacttagg tctttcttca
     6901 ggataaaatc ctgttatcca tccgtggtct atagcttttt tatttaaact actttgagct
     6961 gtaccagttt taactccaca accaccttca atatctttta ggtctttagc tgtaccagtc
     7021 ctagatacag acttcatata ttctttaact tgtgtacaca cataaggaga aataagttct
     7081 tctttttttg aagacttata agtttttatt gtattcatat tattgtccac aagactttta
     7141 tataagtata gtggtttgaa tgttccatta ttagctatta tttgagtcat ttgatttatt
     7201 tgaagtggtg taaattctat attttcttga cctatagcta aatttcttat tgatatgttt
     7261 tttggagctt ctctaatttt ttcttcatca agaccaatat caaccttttc aaataaatgt
     7321 aatttttcag cagatttgag tatcttttct tttccaagtt tcatagctat atctaaaaat
     7381 gcaggattgc atgaattaga aaaagcttgt cttaagtttt ggaaaccatg accatctctt
     7441 ttattacatc gcagtatttc attagtattc ccaactttag tcttacctgt acagttgtaa
     7501 gtatagttct catctataac accattttca agtgcagaaa ataaaactac catcttaaat
     7561 acagaccctg gtggatatgt cgcttttata actctatttt caaattctcc attttttcct
     7621 tttaaacttt ttgatatgtc attttggtca aaattaggtc tagagcacat tgcaagaatt
     7681 tcacctgtag atgcttccga gattattgct gctgtaggat tttcttcttt atttagtatt
     7741 tgttctaatt ttttttgaat gttataatca atagtagttt ttagatgtct atctttatcc
     7801 ttattatcaa cagtttttac acttcccttt agtatgttta gagatttttc atttcctgca
     7861 tctccagctt taaaagcact tatatacttt tcatttgaat ttctaagtac actatccata
     7921 ctcttttcaa ttcctgattg acctgattta tcatcttctt ttatatatcc tattgtatgt
     7981 gataataaac catcttttgc gtacctcata gttttttctt ctaccattat tccatttttc
     8041 tcaagttctt ttttcatact cttatcgata tttttagtct gaatctgtat tatagggcgt
     8101 gtcaattgct cttgtacagc tttatatata tctaaatcat tcatcttagt agctttctta
     8161 ataagttcca aaattttata gttattattt aatttttctt tttcaacaat taatacctga
     8221 gatttacttg tatctgtaag cttcttgttg tttctatcat agattattcc tcttccacta
     8281 tttaactcga ctttctcaac gctttgtgat tcaacacttt gtttgtattt atcacctttt
     8341 aatacttgta tatcaactag cctataaatt aaaacactat aaattattaa aattatagta
     8401 aatatacacc agcttctctt tcccactttt ttcaaaaatg gagtcttctt ttttgacatg
     8461 aaatcacccc ttagataatt tcattattga caatttctaa ggggctaata ctatttttat
     8521 caatttttta ttattcttca accttttctt ctataacttt tctaagcata taatctttac
     8581 caacttttac tgatagtttt aacttcacta tttgtctagg atgaggagct gattctatag
     8641 cttctccttc ttcattgtac atttcttcaa ttttagtaaa catagtttct ttatatggtc
     8701 ccataacttc tatttcatca ccaacacaca ttttatttct ttgctcaaca acaataagac
     8761 catcttcatc ttctgtttct ctaactatac ctataaaatc atagtttcta acatatgatg
     8821 ctgattgata attttggtct tctgttgttg gtttattcaa gtaaaaacct gatgtaaaat
     8881 gtctatgact accttttttt agttcttcca tccacattgg attgaacttc cagttttctg
     8941 ggtctctgta aaattcatct attgccattc tgtatgctct tacaacagta gctacatagt
     9001 atgcagtctt cattctacct tctattttta aacttgttat accagatttg attaactctg
     9061 gtatatactc tatcatacat aaatcttttg agttaaagaa gaatgtaccc ctttcatctt
     9121 cgtatattgg aaaatactca ccaggtcttt tttcttccac caaatgatat tgccatctac
     9181 aagattgagc acaagaacct ttgtttgcat ctcttccagt catatagttg cttattaaac
     9241 atctgccaga ataagaaata cacatagcac catgcataaa cgcttctata tccatatcta
     9301 aaggtgtttt tgctcttatc tcagatattt catcaaaaga taattctctt gccactacaa
     9361 ctctttttat tccatgctcg taccagaagt ttgcacttag ataattggtt gtatttgctt
     9421 gtgtacttaa atgtatttcc atattcggaa ttactttttt aatgacacta agcactcctg
     9481 ggtcagctat tataactgca tctattccta tctcttctag ttccaacaaa tagtcttcta
     9541 attgtagaaa atcttcatta tgaggtatta tatttactgt tacgaatact ttcttgcctc
     9601 tttcatgtgc aaaagctact ccctctgcca tatcttcctt actaaaattc ttcgctgcag
     9661 acctcattcc aaaaatttct ccacctatat aaacggcatc tgctccatat gttatagcta
     9721 ttttcaatct ctccaaatca cctgctggag ctaaaagttc tactttattt aattccatct
     9781 acatcacctc ttttactttc tttataactt agtgctacac catcacctat tggtaccaat
     9841 gatgtgctta aatagtcaca attacatata taatctaaat aatttctcat tcttttaact
     9901 atagtctttt ttcttcttac aacaaaatca tcatgagcaa ccataccttt atacaaaata
     9961 ttatcagata ctaaaagtcc cccatctttt aatttatcta taactaaatc aaaaaatagc
    10021 ttatattgac ctttggctgc atctataaat ataaggtcaa attcaccttg gacttgacta
    10081 agtttttctt ctgcatctcc ttctagtata gttatgttat tgtcaaatcc agctaatttt
    10141 atgttttctt ttgctttttc tatcattttt tcatttctct caacagttat tatatctgca
    10201 tcttttccta taatagttgc aaaaaaaata gaagaataac caattgcaca tcctacttct
    10261 aaaattctct ttggtttttg aacctttaac aagactttta ataaatctga aacttcttta
    10321 tgtattatag gaacactatt ttcctctgca taaatttcta aattttttaa aaatccttct
    10381 ttttctttta atgtcactct tatataatct tcaaccaaat cattaactat attactcata
    10441 aaaacactcc ctaaaataat aaatattcaa gttttatatt aatttattat tttttaaatt
    10501 catattgtta tatatattta cactttcttt attataaatc taagaataac aattttcaat
    10561 aaaaaagccc aacattattc ttaccctgtt agacttttta taatcatggt ttattttttt
    10621 gtattttctt tatttgttgc ttttgtatct tttgattgtt tatcccttgc ttctttatat
    10681 tctttaacgt attttaaatg gtcttgatag tttgtactat aattatttcc accatcaatc
    10741 ttagctacaa aatataaata gtctgttttc tctggatata atgctgcttc aatagatttt
    10801 ataccaggat ttgatattgg tgtaggaggt aatcccttat ttttataact attatatggt
    10861 gaatcaattt ttaaatcatc atatgttaca attttctttc tctcttcaaa tatatattgt
    10921 attgtagcat ctgattgaag aggcatacct accttaagtc tattataaaa cacacttgct
    10981 attataggtc tgtctttgtc aagaacagct tctttctcaa ttattgatgc catttccatt
    11041 acttcttgaa gagtcatatt taattctttt tgtttctttt taaacttgtc cgtgtattta
    11101 ctgtcaaata cttttaacat ttcgcttaaa atatcttctt ctgattgttt ctttgaatta
    11161 aaataataag tttctggata caagaaccct tctaaagagg ttattccatc ttccttcaaa
    11221 tatggaaatt tatcataaaa ttcttgaggt ttttttatta aattttcaaa agtagcttta
    11281 tctcctaaat ttttacttac taacattgct ataatttctt tagatgtagc tccctctgga
    11341 atagtcactt ttataccatc ctgatatatt ttacctgaaa ctaaaagact tattatatca
    11401 ttgtttgaat acgattgatt aagtaagtat gttcctgatt ttatagacgg agctttattg
    11461 ctaaccttta ccaaaagttt aaataatagt tcattcttta ttaatttatt ttcatataat
    11521 atgtcagata tttttccaac agatgctcca cttggaacgt cgataattac atcttttttg
    11581 ttatttttat cataaggacc tatttgtata aacacaaata tacctgctaa gataatcaaa
    11641 ataacaatta ttaatactgc tattttcaat ctattttctt taaagttcat aatataatct
    11701 actccttatg tatatttatc cattttctat tataaaacta gatactttaa ttttcaatca
    11761 tcttgtagaa taaacttatt t
//
LOCUS       DAEORZ010000129        11735 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEORZ010000129 DAEORZ010000000
VERSION     DAEORZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.130"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            68..1108
                     /locus_tag="KRH39_002995"
     CDS             68..1108
                     /locus_tag="KRH39_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8512882.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1464..2786
                     /locus_tag="KRH39_002996"
     CDS             1464..2786
                     /locus_tag="KRH39_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG8512883.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3083..4444
                     /locus_tag="KRH39_002997"
     CDS             3083..4444
                     /locus_tag="KRH39_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG8512884.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4517..5911
                     /locus_tag="KRH39_002998"
     CDS             4517..5911
                     /locus_tag="KRH39_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8512885.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            5973..6443
                     /locus_tag="KRH39_002999"
     CDS             5973..6443
                     /locus_tag="KRH39_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBG8512886.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6446..8662
                     /locus_tag="KRH39_003000"
     CDS             6446..8662
                     /locus_tag="KRH39_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG8512887.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8745..9416
                     /locus_tag="KRH39_003001"
     CDS             8745..9416
                     /locus_tag="KRH39_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8512888.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9779..10993
                     /gene="dpaL"
                     /locus_tag="KRH39_003002"
     CDS             9779..10993
                     /gene="dpaL"
                     /locus_tag="KRH39_003002"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG8512889.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLVNLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11120..>11735
                     /locus_tag="KRH39_003003"
     CDS             11120..>11735
                     /locus_tag="KRH39_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG8512890.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMA"
BASE COUNT         4437 a         1357 c         2388 g         3553 t
ORIGIN      
        1 tatgatataa ttaaatagat aatgtgaatg attattatat agaatactta tttggagttg
       61 atattttatg gacaaacaca gtgattcagt aatgtatggt ggaatggaag tatataatat
      121 ttggggaaca acaacagtag actccataat tggagagtct gatgtaatgc gtgctcttaa
      181 gaatagaatt tgtaaaattg gaaatagtga ttctactgta gctataactg gagaaagtgg
      241 cactggaaaa gaattaatag caagagccat tcatctcgca ggaaacagag ggaataaaac
      301 attcgttgct ataaactgtg ctgctatacc agaaccttta ctagaaagtg aactttttgg
      361 atatacaaaa ggagctttta gtggagcaga ttcaaaaggt aaaattggta agtttgaatt
      421 ggccaatgga ggggttgtat ttttagatga gataggagat atgccaattc aactacaatc
      481 aaagttattg agggtattac aggaaaagaa gtttacgaga ataggttcaa atgaactaat
      541 tgatatagat gttagaatca tatcagctac taatagagat ttatttgagt tggttaagga
      601 aaataaattt agagaagact tatactatag attgaatgta atacctttag aagtgcctcc
      661 acttagagaa agaggagacg atatacaaat attaataaac aattttatag acaaatattc
      721 gagaaagatg aagaaaaacg tgtaccatat agagccagat gtggaacaaa tgttgcttaa
      781 gtatccttgg ccaggaaata ttagagagtt ggaaaatact atagaactgg cattaaacct
      841 tcttgaggaa gatggaataa tacataaagg cataataaat agaaagatac ttgaatattt
      901 taagttaaat aatataaata taaagttact tgaggaaagt gactatgaga taagtatgga
      961 acaagacata cttacacttg aagagtttga aagagcctat ataaagaaag tgttaaagtt
     1021 ttacggaaat gatactaagt ctaaaaaact agctgccaaa aaacttggaa tatcactagc
     1081 tacattgtac agaaaaatag atgtataata attaggcttt ttattctcaa ttctaaaatt
     1141 tgaaattata aaattgataa aaaacacttg gaaaactatt ttctaagtgt ttttttgtgt
     1201 aatagcattt tattatccaa aaataaaaaa aatttaaaag aatttaaaaa aataaatgct
     1261 ttaataaatt atcaaatact aacaaatata ataaaaatta tatatgtaaa acaattatgt
     1321 taaaaaattg gcattataat tgcttcatat taagttgaga tgacaaaata aaaaaagaaa
     1381 ggatgttgtg tgagataata ataactgtca ttaaaaaata gttgggagtg ataaccttga
     1441 gtaaatatca aaagaatgtt tctatgtttg atattgatgg aaggccacca attttaaagt
     1501 gtttgccact atcaatgcaa catatactag caatgatagt tggaactgtt acagtaccta
     1561 tagtcgtagg aggagctgtg ggtgttagcc ctacacaaat aacactttta gtgcaatatg
     1621 cattaataat agctggtata tcaacactta tacaactata tccaatagga aatattggtt
     1681 caagattgcc tataattttt ggagttggtt ttacttatgt accaacactt gtagcagtcg
     1741 gaggaaatta tggattagca agcattttgg gtgcacaatt aataggggga gtggctacag
     1801 tattaatagg gatattcata aaaaagataa gaaaattttt tccgaatata gttgcaggaa
     1861 cagtggtttt tacaattgga ttatctttgt atcctatagc aataaattat atggcaggtg
     1921 gtgtaaatag tcctacttat gcctctatgc aaaactgggc agtagcggct ataacattag
     1981 tggtagttat aggtcttagt caatttgcaa aaggatatgc aaaattggca gcaatatttt
     2041 tgggaattat agttggatat tgtgtatctt tagttctggg tataataaat tttgacccta
     2101 ttagagaggc aacttggttt gcgataccta aaccgcttga atttgggatg gaatttgata
     2161 tgcctgtaat aatctcagta gtaattgtaa gtataatcaa tgctgttcaa gcaattggag
     2221 acttatcagc tactacaatg ggaggcatgg ataggaatat cacagataag gaattatcaa
     2281 gtggagtaat aggaagtgga atttgtacta tgataggagc tttgtttgga ggtttaccac
     2341 catcatcata tagccaaaac gtggggatgg tagctatgaa caaagtaata agtagatttg
     2401 tgcttggaat agctggtata tttatgttat tgtcggggtt tgtgcctaaa tttggtgcat
     2461 taatgactac tataccatat tctgttttag gaggagctac aatatctata tttagtatga
     2521 taacaatgac aggtataaag ttaattatta cagaagagtt gtcatcaaga aatattacca
     2581 ttgttggttt ggcaattgcc ttaggcatgg gaataacatc agtcccagca gcccaagagg
     2641 catttccttc atttgtaaag ttagtatttg gagaatctcc aatagtaatt gcaacattgg
     2701 ttgcattcgt tttaaatcta ataataccaa ataaaagtct agaggatgag gctaaagaaa
     2761 gagctgttat ggaagagact aattaagata taataattta aaagaattgt ttaaattatt
     2821 tagtatgaga ttgttagatt atttttgata gctattcatt ttaataaaaa atttaacaaa
     2881 taaatatata ccaggcaatt aatgttcatt taagttgaaa atcggggaga caatatttag
     2941 aaatttgtta attttttata aaaatagctc agtttagctg tgtttatcgg ttgagcaata
     3001 aattcatggt cataaaaata tttatagaaa ataattataa aaatatttag tcaagatatt
     3061 tatgtatggg aggatttatt ttatgaaaac acgtaatact tcaaaatatg atgtcgatgg
     3121 gataccacca ctaaaggaat caataccttt ggccttacaa catctgttag caatgatagt
     3181 aggaaatatg gtacctgcga tattaatagc aaacgtggta ggacttaacc aggggcaagc
     3241 aacaatgttg attcagggtt ctatgttagc agcaggttta gcaacgtttt tacagttata
     3301 tccaatacct ttatttaaag gatttaagtt aggttcaaga ttacctgtaa tgatgggaat
     3361 gagttatgta tttttaggag cttgtctttc agtagcagct aaagatggtt tagcagctct
     3421 atttggagca cagattgctg ctggggtaat cgtattcttt gtaggatttg gagttaagaa
     3481 aattagacat atattcactc caatagtttc tggaactata atagcatgta tgggacttgg
     3541 actattcgca acagctataa agaatttagc tggaggagaa ggcacacaaa catttggaag
     3601 cccaataaac ttcacagtag gagttatagt tgcttttgta ataataatga taaataaata
     3661 cggtaaaggt ttagtaaaaa attcttcaat attaatagga atattagtag gttatgctat
     3721 atctttagtt ttaggattgg ttgatttctc agccgttcaa ggagcagcta tagtatcttt
     3781 accaacacca gcagcttttg gattagaatt tagaccagag ttaatagtaa tgtttactat
     3841 tatttacata ataggtatag ctgatatgat gggtgcctgt acaatagcta cagtaggagc
     3901 aatggataga gaggtaactg acgaagaatt agcatcagtt gtattaggaa attctataac
     3961 ttctataatt tcatcactat ttgcagctct tccaactgga gtatttagcc aaaatacagt
     4021 aattgtatct atgaacaaag ttacaagtag atttgtaata gcactagggg cacttgtatt
     4081 attactagca gggatatctc cagcattggg tgcaataatg acaactatac catcttgtgt
     4141 tgttggtgga gcaactttag tagttttctc atcaatagca atgtctggtt tctctattat
     4201 gagtatggat ggattcactg aagaaaacaa tcttatagca ggtgtatcta tagcaacaag
     4261 tatgggtctt acaactgctc cacaagtttt agaccaattc ccagaaacaa ttggaactgt
     4321 ccttggaggg tcaagtatag tttctggagc aataatagct atattacttc aaacattatt
     4381 taaattaaag tcacgtaaaa gtgctgaaaa cgttacttca aatcttgaag aaaatattgg
     4441 ataaatgtat tagatataat atgagaaaac catatggtaa aatgttgtaa tgaaagtatt
     4501 caaaggggga tttaagatga ttgatatact aataaaaaat gcaataattg ttacagttaa
     4561 taaagacaga gatgttatat ttgatggagc aatagctatt caaggtgaca gaatcttgga
     4621 cataggaaat acagttgata tcgagcctaa gtatcttgat gctaaaaaag ttatagatgc
     4681 taatggaaaa gtaatattcc ctggctttat aaatactcat aatcacctgt tccaagtgtt
     4741 attaaaaggt ttaggtgatg atatggcact tcatgagtgg ttaaatacaa tgatgtttcc
     4801 atcagcaaaa ttcttaacag aacaagatac ttatgatgct gcaatgcttg gatgtatgga
     4861 aggtttaaaa agtggtataa ctacaatggt tgattatatg catactcaca atagaccagg
     4921 acttacagat ggaatagtaa aggcatataa agatttaggc ataagagggg ttgtaggtag
     4981 aggttgtata gatttaggta ttcacaaaga gcttatagaa gatgtggaaa cggttgaaaa
     5041 agatttgaga agggtattcg aaaaatatca taattcagaa aatggaagaa taaaaatctg
     5101 tgtagctcct tcttcaatgt gggctatatc tgaagaaatg ggtaagatgc tttggaatat
     5161 agttaaagaa tatgattcat attttacagt tcatatatct gaaacagaat ttgcaagaac
     5221 agcaacaaag gatatacatg gagaaataga tataaaatta cttgaaaaat ggggtatagt
     5281 aggtccagaa gttgtagcag tacattgtgt atgtataact gatgaagaca tggaaatgct
     5341 taaaaagtat gacataaaag tttctcataa tgtagcaagt aatatgtatc ttgcttctgg
     5401 tgttgcacct gtgccagaaa tgttaaaaaa aggtataaat gtgagtttag gtcttgacgg
     5461 tgcagcaagt aataacgcac aagatatggt agagcttatg aaactaacag ctttacaaca
     5521 caaagtaaat aattgtgacc ctctggcaat ttctgcagag aaagttcttg aaatggctac
     5581 aattgaggga gcaagaactc ttagaatgga agatgaaata ggttctttgg agattgggaa
     5641 aaaagctgac ctagtaatat ttaactcaat gctttctcca aaatcaatac ctcttcacaa
     5701 tcctgtatca actttggtat actcagctag tatgcacaat gtagagagtg ttttagttga
     5761 tggaaatgta atacttgaaa atggaaaagt aactacaatt gaaagtgaag aacaagtgtt
     5821 gcttaatgca caacatgcag cagaagaatt atgtaagaga gctaatgtaa ctaatagaat
     5881 ggaaggacat aagtggaata gcttatacta atattaaaat aagattgtaa taatattcta
     5941 ttacaacatt agtttatctg taggaggaga aaatggaaaa aataaaatta aatattaatg
     6001 attacatgta tgaaatagaa gttgaaaaga atgaaactct tttgcatgta cttcgtgaaa
     6061 aattaaatct aactggagcg aaacaaggtt gtaatagtgg cagttgtgga gcatgtaaag
     6121 ttttgataga tggagttgac atgaaatctt gtaaacttct tgcaaaaaac tgcgaaggta
     6181 aagttatata cactatagaa agttttgcta atggagaaaa tcttcatcca atacaagaag
     6241 cttttataga agttggagct gtacaatgtg gatattgtac tcctggtatg attataacaa
     6301 cacaagctct tttaaataga aatgcatctc ctaatgaaga tgaaattaga gaagctttta
     6361 aagaaaatct ctgtagatgc acaggatatg taaagattat agaagctgta aaattatctg
     6421 cagctagatt agggggtaga taattatgga aaaggtaata ggaaaatcat accctctaaa
     6481 agatgcagct ttaaaagtta ctggtcaaat gaaatacgtg gctgatatga aaccacaaaa
     6541 tgtactttac gctaagatgc ttttaagccc tgtagcacat gcaaaaataa aaagtataga
     6601 tacaagtgaa gctgagaaag tagtaggtgt tagagctata gctacttatc tcaatacatc
     6661 acaaagaaaa tacaatggtt cattaagatt ttatgagcac aaaatacctg aaaatgaagt
     6721 tatattttca gatacagtta gatttgttgg tgaccgtgtt gcagcagtag ctgcagagac
     6781 tccagaaata gcagagaaag ctgtaaaact tataaaagtt gaatatgaag aattgccagc
     6841 tgtatttact atacaagatg caataaaaga agatgctatc tgcatacatg gagattcaaa
     6901 tatagtttct caaaacaaca taaatggggg agatgtagaa aaaggcttca aggaatgtga
     6961 ctatatattt gaagatgaat actctactcc agcaatacat catagtgcaa tagagactca
     7021 tagtgtaatt gcacactatg attataatga taaattaact gtacatacac cttgccaaaa
     7081 tacatttgga tttagaataa tactatctca aatttttggg atgtcattaa ataaagtaag
     7141 agttataaga cctgctattg gaggctcatt tggtggaaaa ttagaactta caattgaacc
     7201 agtagcagcc gcattatcaa aaatgacaag aagaccagtt aaaatggttc ttacaagaaa
     7261 ggaatctatg atatcaacaa gaactcgtca tgggtctttt gtaaaattaa aaactggtgt
     7321 aaataaagat ggtgaagtaa tagcacaaga cataaaaata tatacaaata caggagcata
     7381 tgcatcaagt gctttaaatg ttataggagc acttagtcat aaagtattta aagtttataa
     7441 aataccaaat ataaagttta ctgggatgcc tgtatataca aatacaccaa tagcaggagc
     7501 tatgagaggc tatgggtcac ctcaaatatt tatggctcaa caagctcaat ttgcgaaaat
     7561 agcaaaggaa ataggtatgg atttagtcga ttttcaaaat aaaaatgcag tagagccaga
     7621 tgatgtcgat ataatcttcc acggttctct tggaaatcct agagtattgg attgtataga
     7681 gcaaggtaag aagatgttta agtgggatga aaagaaaaaa cttccaaaag aagaaggaaa
     7741 atatttaaga ggtataggta tggcaatagg tgcacatggt aatggagttt ttggtgcaca
     7801 tagagatgtt gttacattga ctcttaaatt aaatgaagat ggtacgttga cactgctaac
     7861 aggagctcat gatatgggta atggtgttgt aactatgcaa acaatgatgg tagctgaagt
     7921 tttaggaata acacctgata aagttgatac atttgaaact gatacggatg catgtacatt
     7981 caaccttggt gactatgcaa gtagaggggt atttgtagaa ggtggaggag ctaaaaagac
     8041 agcagagaaa ttgaaaaatc ttatattaga agaagcagaa aaattacttg aaacatcaaa
     8101 agaagattta tatctagaca atggatgtgt aattagtaaa cttgatgaaa atgtaaaagc
     8161 atcactttct gatgtcgcag tatatagtca aagtaaatca ttaagagagt taactgttgt
     8221 tgaagattat tcttcaccag ctggacgtac ttcttatggt gtacattttg ctgaagtttt
     8281 agtagataaa gaaactaagg atataaaagt agttgatttt gtagctgtac atgatgttgg
     8341 tagagttata aatcctctaa atttagaagg acaattagaa ggtgggattc atatgggtct
     8401 aggatacgca ttgagtgaag aattggcatt tgatgaacaa ggtagaccta aagctactga
     8461 ttttaaaaca tataaattat ttagaactgt agatatgcca aaatgtcaag ttgcatttgt
     8521 agatggttat gaaaaagctg ggccttttgg tggtaagagt attggagaat gtgccgttgt
     8581 accagttgcg ccagctgttg caaatgcagt atataatgca actgatgtag agatacattc
     8641 attaccaata aaattaaaat agtagaaatt aaagcagaaa taacaattta ttgtatttgt
     8701 aaatagataa taaaaataga tttatttggt gaggagatat tattatggca aagatattag
     8761 gtatttgtgg aagtccacgt aaaggagcaa cagaatatgc agtgttagaa gctttgaaag
     8821 aagcagaaac tatacctggt atagaaactg agtattggtc agttagaggg aaaaaaataa
     8881 gtccttgtgt acattgtgat gcatgtataa gaaaagaaac tatgtgtatt ataaaagatg
     8941 atatccaaga attagagcaa aaaatattgg aggcagatgg aattatagta ggttctccag
     9001 tttatgatat gaatataact gctcaactta cagcagtatt taatagactt cgtccaatgt
     9061 atttagttca tccaggaaaa cttcaaaata aggttggtgc agctataact actggtggaa
     9121 ctagatatgg tggccaagaa ttgacaaaac ttcctataat aaatttcttc ctaatgcatg
     9181 aaatgttagt gtctggaggt ttaggtggat gctatatagg tggtacaata tggagtaaag
     9241 atggaaaagc aaaaggtgcc caagaggatg agacaggtat ggacacagta aaaagacttg
     9301 gaaaaggtgc agcagaggca gttatggttt ctaagtttgg tcttgaaaaa tggaatgtcg
     9361 taaaagaaga attagatgtt aaaaaagatg aaaaatcacc tcttagagac cactaaagat
     9421 ttattatatt attgaatata tacaaatagt gatgtaatag attattgcat cactatttta
     9481 tgttttaatt tagattttat aaattttata ttaaaaaata tagaaacaaa gtgttttgag
     9541 atttattttt aaattttaaa aatgagaatt aaaaagtgag aagatagaaa aggtttttta
     9601 gttattaaat tctacataca ttgtatgaac taaagataga aaagaaaaaa taataaaagc
     9661 attgcaattt ctatactaaa gagtcatgga aaaatatcta gtatatattt agtattggca
     9721 tgttaattgc ttatatatta aagtataaaa atttaacaat aatttggagg tattttagat
     9781 gttaaaagaa attaaatgga aagtaaataa tttgccaaag ggcgataagg aaaattgtat
     9841 caaattttta aatgaagaag aaataactaa ggttagaaat tttcacaaaa gctttcctca
     9901 atataaagaa actccattag taaacctaga aggtttagca aaaaaattag gtgttgcagg
     9961 agtatatgta aaagatgaat cttatagatt tggattaaat gcatttaaag tattaggtgg
    10021 ttcttattct atgggtagat acttagctca aagattagat acagatatat ctgagttagg
    10081 gtatgacaaa ttaacttcaa aagaaataaa agaaaaatta ggagaaataa ctttctttac
    10141 agctacagat ggaaatcacg gtagaggagt tgcttggaca gctaataaat taggtcaaaa
    10201 atctgttgta ttaatgccaa aaggttcttc tgaatttaga ttaaataaaa ttaaaggaga
    10261 aggtgcagat gctagtataa cagacttaaa ctatgatgat gcagtaagat tagctaatga
    10321 ttatgcagaa gctgatgacc atggagtaat ggttcaagat acagcatggg acggatatga
    10381 agaaatacca gcgtggataa tgcaaggata tggaactatg gctcaagaag ctatagagca
    10441 attaaaagaa tatggagtag atagaccaac acacgtattt gttcaagcag gagttggttc
    10501 acttgctggt gcagttcaag ggtatgttgc ttcaatatat gatgaatgcc caataacagt
    10561 agtagtagag gctgatgaag ctgattgtta ctataaatca gcagaagctg gagatggaaa
    10621 accaagattt gttggaggag atatgccaac tataatggca gggcttgctt gtggagaacc
    10681 aaatactata ggatttgaag tattaaaaaa tcatgcagca gcatttgttt cagcaccaga
    10741 ctgggtttct gctaaaggta tgagaacatt aggtaatcct ttaaatggtg atgaaaaggt
    10801 aatttctgga gaatctggag cagttacaac tggtctttta gtagcagcta tggaaagaga
    10861 agatttagca gatttaagaa aagatttaaa attagatgaa aattcaagaa tacttttaat
    10921 aagcacagaa ggagatactg accctgacaa atatagaagt atagtttggg atggagaata
    10981 tccaagtata taaggaatta ctaaaatgca attattagat attggtttac atatttagaa
    11041 gatatataag ttctaaaatt tagaaaatat ataagtatat aaaatacata cattataaaa
    11101 aatctagggg gataatgaaa tgttaaacga acaaagaaaa caagaagtaa ctgaaatatg
    11161 ccaaaaatta attcaaaatc ctagttcttc tggaaatgaa gaaggtgttg taaaagcaat
    11221 agaagagtct tttgaaaaat taggttttga tagttggtca agagatagat atggaaatat
    11281 agtaggatgt ataaaaggta acaagccagg taagaaaatc ttatttgatg gacatataga
    11341 tacagttcca gttccagatg caagtaaatg gagtgttcct ccttttgaag gaaaaatagt
    11401 tgatggaaag atatatggta gaggaacttc tgacatgaaa ggtcaatata ctgcaatgat
    11461 gtctgctgta gcgtattttg ctaaagacac taatagagat tttgctggag aactatatgt
    11521 agcaggtgtt gttcatgaag aaatatttga aggtgtttca gctagagaga taagtaaggc
    11581 tgttcaacct gactatgttg taataggtga gtcttcagag ttaaacttaa agattggtca
    11641 gagaggtaga ggagaaatag tagtagaaac ttttggtaaa ccagctcact ctgctaatcc
    11701 agaaaaaggt gtaaatgcag tttataaaat ggcta
//
LOCUS       DAEORZ010000130        11713 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEORZ010000130 DAEORZ010000000
VERSION     DAEORZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.131"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            203..667
                     /locus_tag="KRH39_003004"
     CDS             203..667
                     /locus_tag="KRH39_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBG8512891.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            674..1168
                     /locus_tag="KRH39_003005"
     CDS             674..1168
                     /locus_tag="KRH39_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBG8512892.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1184..2209
                     /locus_tag="KRH39_003006"
     CDS             1184..2209
                     /locus_tag="KRH39_003006"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBG8512893.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2196..4643
                     /locus_tag="KRH39_003007"
     CDS             2196..4643
                     /locus_tag="KRH39_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBG8512894.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4823..6196
                     /gene="radA"
                     /locus_tag="KRH39_003008"
     CDS             4823..6196
                     /gene="radA"
                     /locus_tag="KRH39_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBG8512895.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6199..7269
                     /gene="disA"
                     /locus_tag="KRH39_003009"
     CDS             6199..7269
                     /gene="disA"
                     /locus_tag="KRH39_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBG8512896.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILGASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7358..8452
                     /locus_tag="KRH39_003010"
     CDS             7358..8452
                     /locus_tag="KRH39_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBG8512897.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8581..10758
                     /locus_tag="KRH39_003011"
     CDS             8581..10758
                     /locus_tag="KRH39_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512898.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(10944..>11713)
                     /locus_tag="KRH39_003012"
     CDS             complement(10944..>11713)
                     /locus_tag="KRH39_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8512899.1"
                     /translation="SGSYVANHWHNSLEIIYITSGDLQINMEGYTYNLKANECMFINS
                     GIIHSTRCIYQNTSILLQVPLSFLNKYIPDFKNCYFDFKVNANDNNYKKNSSKVKLIL
                     ENMREIKLSSPPAANLLFTSLLFELLFELYTNFKISVGNKNMKKTVLDLSKFEPVLEY
                     TKINYKMPISINKIAKVAHLQPEYFCRKFKQYMGQTYLEYLNDVRISHIYKDLINTND
                     TLCSILEAHGFTNTKLFYRIFKEKFKCTPKHIRKKST"
BASE COUNT         4853 a         1197 c         2112 g         3551 t
ORIGIN      
        1 tctaatcatg aagcaaaaaa ttagcatttg gttatcaata aatataataa ataaaaactc
       61 ataattaatg aaagaataaa ttcatatcta aatttctata aaaaatttaa atttatacaa
      121 tttagggctt taaaaattta aataataggt gtaaaataat agtcaaagaa agtcaaaaat
      181 aaaaaggtgt gtggtgatat taatggcaac tatgacagat ataattgaaa aatttattaa
      241 agatttgatg gaagaagata atagcataca aattcaaaga aatgaactag caaacttgtt
      301 tagttgtgca ccatcacaaa taaattatgt cttaacaaca agatttacaa tagatagagg
      361 atattacatt gaaagtaaaa aaggcggcgg aggatatgtt caaatagaaa aaatacgaaa
      421 aagtaaagat gggcatatta gagaactact aaatgaaaaa ataggaagtc agatatccta
      481 taaaaaagca aaagaattat tagaaggttt aaaagagtcg gatttgatta acgaaagaga
      541 attaaaatta atattatatg caatagatga taaatcattg tgtatgccaa tatatgaatt
      601 aaaagagaag gtaaggtcta atattttaaa gaatataata ataggattat ttagtataga
      661 ggagtgataa tttatgctat gtcaaaaatg caataagaat aaagcatctg tatattataa
      721 taagatagtc aatggagaaa aaacagagat gtatttatgt agtgagtgtg caaaagagaa
      781 tacagaaatg aattttaact tagatatgcc tttttcaatg atggatatat tttctaattt
      841 aggttttcaa cctaaaaaag aacttgagga aaagctagtt tgtcctaaat gcaatacaac
      901 atatagtgag tttaagaata atgggagatt tggatgtagt gaatgttata atgcatttag
      961 tagtcaagta aatccaatgc ttcaaaatat acatgggcat atagaacata caggaaaagc
     1021 acctaaaaaa tctttttata agataagtgt agaaaatgag attaaagaac taaaagaaga
     1081 tttagataga gctataaaaa atgaagagta tgagttagca gctcaattta gagataaaat
     1141 taaatattta aaaggttcta ttgactaaga ttggagtgat attatgaaag agaacatagt
     1201 catgaaatca agagttaggc tagctagaaa tttaaataac tatccatttc caaataaatt
     1261 agataaagaa tgtgccatgg aaataataga aaaagtaaaa aatgcgttta ttaattctaa
     1321 cttagaacaa aaagaagaat ttgatttcta taaaattgaa gatttagacc aatctaaaaa
     1381 aatgcttatg gttgaagaac atataataag cccagattta gcagaaaatg ataaaagtgc
     1441 agttatagta aaaaaagata aaactataag tataatgata aatgaagaag accatataag
     1501 gatacaaact atatgtgatg atttaaactt agaatatgca tatagtgtag caaatgaaat
     1561 agatgatttg ttagaatcaa gtttagaata tgcctttaac acaaagctag gttatctaac
     1621 atcttgtcca actaatacag gaacaggaat gagagcttct gtaatgatgc atttaccagc
     1681 attaagtcaa ctaggctata tggatgaact ttataaaatt tcatcacaaa taggcatagc
     1741 aattagaggg atatatggag aacgaacaga agcattggga aatatatatc aaatatctaa
     1801 tcaacttaca cttggaagaa cagagtctaa tattatagaa aatgtaagtg gacttacaaa
     1861 agatgcaatt tcaaaagaaa taaaagctag agaaatattg caaaaaaaat taggtataaa
     1921 actagaagat aaaatattta gatcgatagg gacacttgaa aattcaagag taatgagtag
     1981 cgcagaggca atgagccatc tatcaaatat aaaaatggga atagaaatga actatataga
     2041 taaactagat ttaagggcta tagaacaact tatgataggt atacaaccag cgcatcagtc
     2101 aataatgtat aagagtgatg atgttgagaa tagagatata aatagagcaa catatattag
     2161 agaaactttg gaaaaattaa gaggagggat gaattgtgaa cttcaataga tttacacaaa
     2221 gagctaaaaa ggcaattgat ttagcgtttg aatctgctaa aagtctagga cacaatattg
     2281 tcggaagtga acatatactt ttaggtcttt taagagaaga agaaggtata gctgctaaag
     2341 ttctaagtaa agttggattt acagaagctt atttagaagg caaaatagtt gatatggaag
     2401 gtaagggaga agaaatctca gaagatatag tattaagtcc aaggagtaaa caaatacttg
     2461 aattatcagg aatgtttgca aataaattaa aaacaaatta tattgggact gagcatatat
     2521 tattagcaat tattcaagaa ggtgaaggaa tagctaataa gattttaaat tatgctggag
     2581 taaatgatag gactttagcc caattaacaa ttgatatgat gggtattagt gataaaaatc
     2641 aatacaaagc agaaaatagt tacacgggca accagaatca agcagaatct aaagtacttg
     2701 ataagtatgg aagaaatctt acactatatg caaaacagaa taaaatagac cctgtaatag
     2761 gtagagaaaa agaaatacaa agagtaatac aaatattaag tagaagaact aaaaacaatc
     2821 cagtgttgat aggagaccca ggtgtaggga aaacagccat agctgaaggt ctggctacaa
     2881 atatagcctt aggaaatgtt ccagaaacac taaaaaacaa aactttatac tctttagaga
     2941 tgggttcatt attggctggg gctaagtata gaggagaatt tgaagaaaga attaaagaag
     3001 ttgtagatga agttgttaaa aatggaaaca taatcttatt tatagatgag atgcacacaa
     3061 taataggggc tgggtctact ggagaaggct ctatagatgc ttcaaatata ttaaagccag
     3121 ccttagcaag aggagaaata caagttatag gtgcaactac aattgatgaa tatagaaagc
     3181 atgttgaaaa agattctgct ctagaaagaa gattccaacc agttatggta gatgaaccaa
     3241 ctaaagagga ctcaataaaa atattagaag gattaagaga taaatatgaa gctcatcata
     3301 aagttaaaat aactgatgat gctataaagg cagctgtaga attatcaact agatatatat
     3361 cagacagata tctgccagat aaagctatag acttgattga tgaagctgca tcaaaagtaa
     3421 gattaaaaga aaatacacct ccttcagaaa taaaaaaatt agaactagaa atagaaaata
     3481 tagataaaga aaaagaagaa gcagtaagat gtcaagactt tgagaaagca gccaaaataa
     3541 gagatgaaca aggtctactt aaaaaacaat tagaagatgt tagagaaaga tggaacaagt
     3601 catctaaaca ttcagattta gttgatggag aagttattgc tgaagtagtg ggcttatgga
     3661 caggaatacc agttaataaa attcttgaag aagaggctga tagacttttg agacttgagg
     3721 aaatactgca caatagagtt ataggtcaag aacaagcagt aaaatctatt tcaaaggcaa
     3781 tcagaaggtc aagagcaggt cttaaagacc ctaatagacc aattggttca tttttatttt
     3841 taggtccaac aggagtaggt aaaacagaat tatctaaggc attagcagaa gtgcaatttg
     3901 gagatgaaaa tcaaataatt agaatagata tgtctgaata tatggaaaaa catgctgtgt
     3961 caagaatgat aggttcacct ccaggttatg taggtcatga tgaaggtggt caattaactg
     4021 aaaaagtaag aagaaatcca tattcagtta tcttatttga tgagatagaa aaagctcatc
     4081 cagatgtatt taatattctg ttacaaattc tagatgatgg tagactaact gattcaaaag
     4141 gaagaactgt agattttaag aatactattg taataatgac atcaaatgtt ggagcatcta
     4201 caattggtag acaaaaaact ttaggattta gtatagctaa aggagacgaa gaagaaaaat
     4261 ctcaatatga aaaaatgaaa gaaaatataa tgggtgaatt aaaacaaaga ttcagaccag
     4321 agtttttaaa cagaatagat gatataattg ttttccactc actaaatgaa aatcatatat
     4381 caaaaatagt acttttaatg gcagcgaaac tacaagagag attaaaagaa atggatataa
     4441 aattagaaat gagtgatgag gctgttaaat taatttctaa atctggattt gacttagaat
     4501 atggagcaag acctcttaaa agagctttac aaaaagagtt ggaagatgag ctatctgaag
     4561 caatcttaaa aggtaatgtg aaaaagggaa gtaatgtagt agcaaaagtc aaagatgaaa
     4621 aaatagtttt tgaaactaaa taatttaata gagttgcatt ttaaaatatt gcaaatataa
     4681 gttttataat ctaaacaaaa ttttaagggc aatgaatcta taataattca ttgtccttaa
     4741 ttattttatt taatctttac atattgtctt tcagatatgc taaaattata gtatagaata
     4801 aaaaattaag gatgataaaa gtatggcaaa aataaaaaca aaatatgtat gtcaatcttg
     4861 cggatatgaa acagctaaat ggcttggtaa atgtccagaa tgtacaaaat ggaatacatt
     4921 tgtagaagaa atagaccaaa aaagcaccaa aaaagaggtc tttataatag ataaatcttc
     4981 atctaaacca gtcagtataa actcaataga gagtaaagaa gaagagcggt ttacaacaga
     5041 tataaatgaa ttagatagag tgcttggagg aggtatagtt aaaggctctt tagtgcttgt
     5101 tggtggtgac cctggtatag gtaaatcaac acttttaatc caagtatcta gtaatgttgc
     5161 caatttagga aaaactgttt tatatataac tggagaagaa tcagaatctc aaataaaaat
     5221 gagagctaaa agattaggta ttaactcaga aaatttatat atatttgctg aaaataattt
     5281 aagtataatt gaatcttatt tagaaagtgt aaatccagaa ttaataattc tagactcaat
     5341 tcaaactgtc ttcagtccag aaatatcctc ggcaccagga actgtcagcc aaattaaaga
     5401 aggtacttca aaattcatga aaatttctaa aaaaatggga atttcaacgt tcatagttgg
     5461 gcatgttaca aaagaaggtt cactagcagg accaaaatta ttagagcata tggttgatac
     5521 tgttctatat tttgaaggag aaagatataa tacttataga ttagtaagag ctgttaaaaa
     5581 tcgatttggt tcaactaatg aacttggagt atttgaaatg agggatttag gattagtaga
     5641 acttgataat ccatctaaaa ttttaatctc tgaaaaacca aaagatgtag caggttcagt
     5701 aataatatct actgtagaag gtacgagacc aatgttactt gaattgcaag cattagtttc
     5761 tcctacaagt tttggtatac caaaaagaac atctacagga gtcgattaca atagagtagg
     5821 tatgttatta gcagtattag aaaaacgtgt tggtttgcaa atacaaaatc aagatgtcta
     5881 tataaacata gttggaggaa taaagataaa tgaaccctca attgatttag ggatagccat
     5941 ttctgtagca tctagtttta gaaatatacc gatagatgaa gatatagctg ttacaggaga
     6001 agtgggactt acaggagaag ttagagcagt aagctttata gaaaagagaa tagctgagtg
     6061 taaaaaactt ggatttaaaa agatagtagt tcctagaagt aattatgatg ttgtaaaaga
     6121 gaccaaaggc atagaaatat ggccagtaga taacttaaga caagctataa atatagtact
     6181 tgggaggaat caataaatat ggagaatttt ctagataata aaaatatgct atatgcatta
     6241 aaaatgatat ctcctggaac tccacttaga ttaggtctaa acaatgtact aagagctaag
     6301 actggtggat taattgtaat tgcaacaaac gaagatgtaa tgaaaatagt agatggagga
     6361 tttgctataa atgcagaata ttcaccatca tatctatatg aattagctaa aatggatgga
     6421 gctatagttt taagtggtga tgtaaagaaa atattatttg ctaatgcaca acttatacct
     6481 gactatttta tagaaacatc agagacagga acaagacata gaacagcaga aagagtagca
     6541 aaacaaactg gtgctatagt cataggaatt tcacaaagaa gaaatgttat aacagtttat
     6601 agaggaaatg agaagtatgt agtcgaagat atatctaaga tatttactaa ggcaaatcag
     6661 gctatacaaa ctctggaaaa atataagaca gtattggacc aagctgtaac aaatttaaat
     6721 gccttagagt ttaatgattt ggtaactatt tatgatgttg cattagtcat gcaaaagatg
     6781 gaaatggtaa tgagagttac aagtataatt gaaaaatatg tgatagaatt gggtgatgaa
     6841 ggaactttag taagtatgca attagaagaa ttaatgggta caaccagaat agaccagaaa
     6901 ttaatattca aagattataa taaagaaaac acagaaataa aagaacttat gaaaaaggtc
     6961 aaaaatttaa attcagaaga actaatagaa ttggtcaata tggcaaaact attagggtat
     7021 agtggttttt cagaaagtat ggatatgcct ataaaaacaa gaggttatag gattcttagc
     7081 aaaatacata gactaccaac agcaataata gaaaacttag taaattattt tgaaaacttt
     7141 caacaaattt taggtgcatc tattgaagaa ttagatgagg ttgaaggaat aggtgaaata
     7201 agagcaacat atataaaaaa tggactcata aaaatgaaac aattagtctt attagataga
     7261 cacatatgat gaattttaaa tgaattttac tacttttttc ttgaatataa tatacacttt
     7321 ggtctataat agataagtat aactaggggg tgaagcattg ataaggaaag taacaagaat
     7381 attgtttatt ttacttggat ttacaatagg tataacaacc tatttaacat taatgaaaga
     7441 ttttgaaata ttaacatttg gaaaagaaac atatggttat atcgcagctg tagtagcagg
     7501 tataataata gccattttag gatatcttat agaaccatgg gttgtaaata aagtaaaaga
     7561 aattgctaaa atagtggata aagaattgtc aaaatatcct cagactgata tattgttagg
     7621 gtctatgggt cttatagtag gatttgttat tgcatattta ttaagtggat tagtaaatag
     7681 aataccaata gtaggtggaa tactatcctt actattatat ttattcctag gatatcttgg
     7741 aatgaaagtt gcgcttaaaa gtaaaaatga tttatttaat attggaaaac taggaagatt
     7801 agcaaatcct attaaggata aagataagga taaagaaaat aaaaaagaag taaaagccat
     7861 tcctccaaaa gtattagata caagtgtaat aattgatgga agaatagctg atatttgtaa
     7921 gactgggttt atagaaggaa aacttattat tcctgctttt gttttggagg aactaagaca
     7981 tattgcagat tcttcagatg atttaaaaag agtaagaggt agaagaggtc tggatatatt
     8041 aaatataatt cagaaagaat taaatataga agttgaaata agtgagagag actttgatga
     8101 tatagcagaa gttgatagta aattattaaa gcttgctcag gttttaaatg gaaaagttgt
     8161 tacaaatgat tataacttaa ataaagttgc tcaattccaa ggtgttgaag tattaaatat
     8221 aaatgagtta gctaatgcta taaaaccagt tgctatacca ggtgaagata tggttgttca
     8281 agtggttaaa gaaggaaaag aggctcaaca aggtgttgca tatctagatg atggaactat
     8341 gatagttgtt gatggaggaa gaaaatatat gaatgagact ataaaagtac tcgttacttc
     8401 tgttcttcaa actcctgcag gtagaatgat atttgcaaaa cctaaaaatt aatatgttca
     8461 aagaaaccta taatttgggc tgtttatttt aaatagatgg tccaaattta tttatttata
     8521 acgataatgt ttaatgtatt cttaaagatg gggatttaaa tcagaaagca gggagaattt
     8581 atgagaaaaa agattatatc tttcttagca acatttatca taatattaac atcagcaagt
     8641 cagtatagtt ttgcagatga tatatcaaca cgtggaaaag taatatttat agatatgaat
     8701 agaactagta tgagtaatat gttaagaata aaatcattaa gagaagaact agacaataga
     8761 gggtatattg gcttgatgaa tataagaggc gataaaggaa gtgatgatag aaggtcatat
     8821 gctagtatgg gtgctggtgg tagagctaat gtggctaatg aagaagatat taattttgaa
     8881 agctcgagta aggatagaaa tatagttttt gaatcagcta caggaaaaag tgcaaaaggt
     8941 ataaataatt taactataaa taagtctata aatgaaaatt taaactttgg agagtatggt
     9001 tctgtattag ggtcattagg acaaagttta tctgataatg gattaaaagc atctgtattg
     9061 ggtaattcag atataattga aaatggtcaa cttataaaaa atagaaactt atgtctaact
     9121 gcaatggatg aatatggaag aatacctaat ggaaatgtag atactataaa taaaaaagac
     9181 ttatctatgc cttatggaat atcaactgat tatgataaat tgattgtaga gactaaagaa
     9241 gcttacaaaa ataatgatgt gatttttgtg gagcttggag acacatatag attagattta
     9301 tataaaccaa atttaaatga aaaaacatat gagagtatga aagataacat agaaaaaaat
     9361 atagatatat atttaaaaaa tatattttct atggttgaag aaaatgacac tgtctacata
     9421 gcaagtgcat ttccaagtga tttagattat aaaaataaaa gaagactttc gccaataata
     9481 aaattaaatg gagaagggaa aggtatatta tcatcttcaa ctactagaag agaaggtatt
     9541 gtaacaaatt tagatgttgg ggttgaaata ttagacaact ttaatataaa aaatcaaaat
     9601 atggttggaa gaaaatatga acttattaac agagatgata ataaagaatt tttgatggat
     9661 gaatatcaaa agatagtatc tatttcaagc ataaggtcta caatactgaa tggttttgta
     9721 agtatagttt tcttgtcatg gatagttgct atgattgcaa ttttatttag aaagcatata
     9781 tcaaaaaatt ataaagaaac aacatttttt atattaaagg aacttttaaa aataggaata
     9841 gtaatgccac tgtcatttat gataacacca ataatgaatt tcaaaacacc attagcaata
     9901 tctttaggaa taattataat aacacttact atatatctta taagtaaagt tttaataaag
     9961 aacgatttaa aaaacatgct gttttttact ggattaacca tagtaattat ggttattgat
    10021 gctgggtttg gctcatattt aatgaaaagc aatgttatga gttatgattg tataattgga
    10081 gctagatatt atggagtagg aaatgagtat caaggtgtgg caatcggtag tgcaatattt
    10141 acatttgcaa ttttacttac atataaaaat ataccaaagt ggtctataat agttttttct
    10201 ttggttatct taattacaag tgcatctcca attatgggag caaatgttgg aagtgctatt
    10261 tctgaatgtg ttgcattttt actatttata ctattgatat ataatgtaaa gatagatttc
    10321 aaaaaaatcg tattattagg tatagcagta gtatttgtat taggagtttt tgtagcaata
    10381 gatatgattt tgggaagtaa ttcacattta ggtatgtttg taaaagaaat ttactttaat
    10441 ggaccaggag aaataataca aacatttagt agaaagatag agatgaattt aaagctagct
    10501 caaaccagtg catgggtaaa tatactgtta actggaatag gtgtaatttt agttcttatg
    10561 attaatcaga ttagatactt taagcaactg atggatgagt atccaattgt atttaaagga
    10621 tttatagcat caatagcagg atgtttagta actcttttag tgaatgactc tgggattgtg
    10681 tcatcagcta cagcatttat ttatgtaatt gtacctatga taacattaag tataaatctt
    10741 acagcattaa aagaataagt actgtatgaa taatatgtaa atattaatac attaatttaa
    10801 cttctttatt tacaaaataa agaagaaagt ttataaaaaa taaattataa taaaaaagag
    10861 tttatctcaa aattgattat tctaatcaaa ttttaagata aacttttttt attataaaga
    10921 taaataacat ataacctcat attttaggta gatttttttc ttatatgttt aggagtgcat
    10981 ttaaattttt ccttaaaaat tctgtaaaat aattttgtat tagtaaatcc atgagcttct
    11041 aatatactgc ataaggtatc atttgtgtta attaaatctt tataaatatg agaaatacgc
    11101 acatcattta aatactctaa gtaagtttgt cccatatact gtttaaattt tctacaaaaa
    11161 tattctggtt gtaagtgtgc taccttagcg attttgttaa tagaaatagg cattttgtaa
    11221 ttaatctttg tatattctaa aactggttca aattttgaca aatcaagtac tgtttttttc
    11281 atatttttat tacctacaga tattttaaag tttgtatata attcaaataa aagttcaaat
    11341 aataagcttg taaagagcag attagctgca ggaggagaac taagtttaat ttctctcatg
    11401 ttttctaata taagtttaac tttacttgaa ttttttttat agttattgtc attggcatta
    11461 accttaaagt caaaatagca atttttaaaa tcagggatat atttgtttag aaaagacaat
    11521 ggtacttgca ataatataga ggtattttga taaatacatc tagttgagtg aataattcca
    11581 gaattaatga acatacactc attagctttt aaattataag tgtaaccctc catgttaatt
    11641 tgtaaatcac cagaagttat ataaattatt tctaatgagt tatgccaatg atttgctaca
    11701 taactaccac tat
//
LOCUS       DAEORZ010000131        11704 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEORZ010000131 DAEORZ010000000
VERSION     DAEORZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11704
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.132"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            110..739
                     /locus_tag="KRH39_003013"
     CDS             110..739
                     /locus_tag="KRH39_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512900.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            740..1522
                     /locus_tag="KRH39_003014"
     CDS             740..1522
                     /locus_tag="KRH39_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512901.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1515..2393
                     /locus_tag="KRH39_003015"
     CDS             1515..2393
                     /locus_tag="KRH39_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512902.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNKEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRRLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2426..3385
                     /locus_tag="KRH39_003016"
     CDS             2426..3385
                     /locus_tag="KRH39_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG8512903.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3699..4391
                     /locus_tag="KRH39_003017"
     CDS             3699..4391
                     /locus_tag="KRH39_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8512904.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4401..5405
                     /locus_tag="KRH39_003018"
     CDS             4401..5405
                     /locus_tag="KRH39_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8512905.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            5673..6524
                     /locus_tag="KRH39_003019"
     CDS             5673..6524
                     /locus_tag="KRH39_003019"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG8512906.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6628..7374
                     /locus_tag="KRH39_003020"
     CDS             6628..7374
                     /locus_tag="KRH39_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512907.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7374..9290
                     /locus_tag="KRH39_003021"
     CDS             7374..9290
                     /locus_tag="KRH39_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512908.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDDECISLLSSSLPGNKNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            9569..9802
                     /locus_tag="KRH39_003022"
     CDS             9569..9802
                     /locus_tag="KRH39_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG8512909.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSIFISTKW"
     gene            9998..10426
                     /locus_tag="KRH39_003023"
     CDS             9998..10426
                     /locus_tag="KRH39_003023"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBG8512910.1"
                     /translation="MIKKLNSKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(10881..11651)
                     /locus_tag="KRH39_003024"
     CDS             complement(10881..11651)
                     /locus_tag="KRH39_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512911.1"
                     /translation="MKVNENNIDEELDELSRKVYLESPDFWISISDKIIDKVFDEMLL
                     FFAVKYNYFNIVCFAIENNYLNLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYLLSL
                     NKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDITYKF
                     SSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGIIAK
                     KKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
BASE COUNT         4590 a         1121 c         1953 g         4040 t
ORIGIN      
        1 tagataaaaa ttgttttatt aagaaaaaat taatatattg acaaaattta agtgctcatt
       61 taaactatta attgataata atattcaata ttaaataata ggaggcgtta tgaataaata
      121 tcttctaata aatccagtgg cagaaagaat gtatggagaa aagtttcacc ttattaaagc
      181 aaatttgata aaaaaggggt acataatagc agaatgtgaa ccacagttag aatatgtaaa
      241 aaagcagtac aaagagtata caaaaaagac tgaaaataca atgttggatt gtagatgtcc
      301 agaaagtata agtcttttaa aaagaaataa tttgataaat gattttgaag tacctatgat
      361 agaaccaata ttagtaagga cttgtagggt tttatacgat aaatatataa aaagtaaaga
      421 tgatatgttg gtagtaacct gtccatgtac acaattaaga gactttgcaa gtgaaaagtt
      481 taaggataag ggtaatgcta tgttttatac atggaaagag tttgctgaac aagaaggtgt
      541 aaaatcactt ggaaaaatag atgaatcacc aattccatta ggatatttca aagatacttt
      601 tgaaaaagtt cttgaagtat caagtgaaga cgaaatatta agtgaaatac aaaaaataag
      661 aaatggcttt gaagataagt atgacatagt agaaatgttt tattgtaaag atggttgtaa
      721 taatggagat ggtttgtaaa tggtcaataa atttaataat aagtttttaa ggctagtaaa
      781 gagtgttctg atatttttta taattctttt attatggaaa attacaaatt atcttggtat
      841 ttggagtgat tatatattac catcacccga aaaggtatat agtacatttt taaatatgat
      901 aagtgatggt tcaatattta ttaatgtata tgcaagtatg aaaagagttt tgataggttt
      961 tgcaataagt actgctatag gtgtgccttt agggatattc tttggaattt atagtggagt
     1021 atacgaatac tttaaatctc taataaattt tttaaggaat acacctcccc ttgctttaat
     1081 accaatgctt atattatggt ttggtatagg agaggaatca aaaattataa ttatagtttt
     1141 agcatctttt ttcccaatct ttacaagcac attgaaaggg attaaaaatt gtgactcaaa
     1201 actgattgaa gtgggtagag tatttgagtt ttcaaagctt cagataatat ttaaaataat
     1261 aataccaaat gctattcttg atattgctgt tgggttaaag ttagctcttg gatatagctt
     1321 tagagcaatt attggggcag aacttgtagc agcttcatct ggtcttggat acttgatatc
     1381 tgatggaaaa gaaatgtcta gaacagatgt tgttattgta ggtataatcg taattggact
     1441 tttaggaata attacagatt acatattttc gattattgtg aagaaggtaa gtaagggaaa
     1501 gatggtggaa gcatatgaat aatttagtat gtgactctgg atatataatt agaaatttat
     1561 ataaaagtta taaagttgac aataaagaac atgtagttct tagtgatatc tctttaaaca
     1621 tagatagaaa tcatataaca gtaattttag gtgaaagtgg ttgtggaaag actaccttgc
     1681 ttagagcaat tgcatcactt gaaggtataa atagtggtga aattaggttt attaatgata
     1741 ataaagagta tagacctaat gttggttttg tttttcagga gagcagatta atgccttggt
     1801 taaacgtaag tgaaaatata gcttttcata atcaaagaaa aaatactatt ttaaacaagg
     1861 tgcttagaaa atttaaacac aataaaagaa ttagcagtaa aattataaat gataatttta
     1921 gaagattaga aacaaatgat aatttgaaca aatcaaataa atataataga aaaagtaata
     1981 ttgatgtaga aaaatatctt aagatgatga acttgcagaa atttaaaaac tcttatccaa
     2041 atgaattgtc aggaggtatg gcacagagag taagcatagc tagagctctt tctttcaatc
     2101 cagatatgtt gcttatggac gagccttttt cggcattaga ttattttaca aggattgata
     2161 tgcaaaatga agttataaga atacataagt caactaataa aggcgtgata tttgtaactc
     2221 atgatattga tgaagcgtta aagataggca agaaaatagt tgttttcaca gacgaaagaa
     2281 aaataaaaga atttaatata gaagatggtt ataatagaga tttaacttca gattactata
     2341 ttaaattaaa aaaagatatt ttaatgacaa tgaaatcaaa taaaggcatt taaagaaatt
     2401 aataatattt taaggagtaa agtaaatgaa attaaaaaag ctattgatag gtataacgat
     2461 attgacactt gcaacagcat ctttagctgg atgtgctaaa aaaaataatg gagaaaaact
     2521 ttcagaaata aatttaactt atgtaaaatc accattaaat gttccttcta taattcagaa
     2581 acaggatgat ttatttggaa aggaatttaa aaaggataat ataaaagtta acttccacga
     2641 aattactaca ggtccagagc aaacacaagc tttagctgct ggagaaatag acttcttaca
     2701 tgcactaggt ggtacatcag ctttaatagc tgcatctaat ggagtagagt taaagatatt
     2761 aaacacttat agcaggtcac caaaaggatt tatgattttg actaataata attcaataaa
     2821 atcagcagca gatttagtag gtaagaaggt ggcaggccca aaagggacta tattacatca
     2881 ggtattgatt tcagcacttg ataaagaagg attaagtatg gatgatgtgg aatttgttaa
     2941 tatgggtatt ccagaggcat cagcagctct ttctgatggc agcgtagatg ctgcattgat
     3001 tgctggtcca gcagctctta aagctatgaa gtcaggtagt aaacttgtag caaatggtga
     3061 aggtttagta gatggtataa tagtaactgc tgttagcaca gactttgcag agaaacatcc
     3121 agaaatagtt gaaagattta tgaaggttga aaaggaaact ttagaatatg taaataataa
     3181 ttttgatgaa gctatggaaa aagtagctaa agaagttgat ttaagtcttg aagaaactaa
     3241 agaattatat gcttggtatg attttagttt agatataaca gataaagaca tatcttcgct
     3301 tgaagataca caagacttct taataaaaaa taagttacaa gagaaaaaag tgaacataaa
     3361 agaattaatc tataatgcta agtagaaact atgataaaat aatactaagt aagaaatata
     3421 ataaaagttt aagttacctt attaaggtaa ctttttctat tttgaattaa taatttcaat
     3481 ttattttaga atgattagtt gataataatg attaattaat cataaaaaga tgctttttta
     3541 tagagtttta ttaagatgtc taataatatg gttatactta tgaagtcagt aattgtgaag
     3601 ttagtaatat gattaaattt atatattgta aaaagattat aatatattaa aattggactg
     3661 ttatatgatg agtaaatagg tatattagga ggaattatat gagttataaa gtatatattg
     3721 tggaagatga tatatcaata agtacattac ttaaagatta tattgagaaa tatggatttg
     3781 aagtgattgt tgaagagaac tttgatgata taatgattac atttgaaagt tttaagccac
     3841 acttagttct attagatgta aatttaccta aatttgatgg tttctactgg tgtagatgta
     3901 ttaggcaaca gtcgaattta cctataattt ttatatcagc tagagatggt aatgtagacc
     3961 aaattagagc tttagaaagt ggagcagatg attatataac aaaacctttt tactatgatg
     4021 tagtaatggc aaaaataaag agtcatataa gaagggtata tggtgaatat tcacctaaaa
     4081 tagacgaaag aatagtggag ctagatggat taaagtttta tccagaaagg ttagaactag
     4141 aattaaatgg tgagaatcta attataacta aaaaagaagg tatactgatt gaatgtctta
     4201 tcaagagata cccaaaagta gtaagtagag attatctttt agaaaagata tgggatgata
     4261 ttgagtttgt agaggaaaat actttaaatg taaatgttag caggataagg aaaagatttg
     4321 aggaattagg tatagataat gctgttcaga ctgtccgagg gttaggatat aaattaaata
     4381 agaattggta aggagaaagt atgaagttat ttttaaaaga ttataaaggt tatattttta
     4441 tatattatat aggcatagtt atgacaatta tttactgtaa tcttatgaag tttattagtt
     4501 ttggagagag tttatatatt cttttattta gttcttttat attgttatgt tttttaatat
     4561 ataagtatta taaaaataga gaagtatata atctgtttga aaaaggagta cattctttgg
     4621 aggagtcatt tttgtattta ggaagctcat tcttggggag aaatatatca gttattttaa
     4681 aacaacaaca taaattgtat caatcaatga tacaagagca taaaaagata catagtgaac
     4741 atttaacttt catcaaccaa tggattcatc agatgaaaac accaatatca gttataagcc
     4801 ttcagcttca agattatgag ggagaagaag taactgaaaa tataagaaaa gaaatagata
     4861 gattaaataa agggttagat atggctatgc attttgcaag ggcagataat tttcagaaag
     4921 actttatagt tgaaaaagcc aaattaaatg aaattgtatc tagtattgta aatgaagaaa
     4981 aaaggttatt tataaaaagt ggaatgattc ctaagattca agtagacaaa tttacttatg
     5041 tttatacaga tgtaaaatgg atgaaattta taataggtca attaattaca aatggagtga
     5101 aatattctaa agattatagt aactacctaa ctataaaatc tgaagaaaat aataaatatg
     5161 taattttaag tataatagat gaaggaattg gaattgtttc taaagattta aaaagggtat
     5221 ttgacccatt ttttacagga gaaaatggtc gtaagtttgg agaatctaca ggtatgggtc
     5281 tttatctagt aaaaaaggta tgtgatgatt taggccataa agtaagtata aaatcagaag
     5341 taggaaaagg tacagaagtt tcaatctact ttaataaaga tgtaaatagt ttaataaagg
     5401 tgtaaatagt ttgataaaga tgtaaattat ctgttacaat agggttttta gttaattgta
     5461 cttttttatt atacaattta ttaaatgaaa tattcatagt taaataatca aaatttaaga
     5521 gagtttattt gcaaacctta catagatgta aggtttgtgt aatgtttgca gatagttaaa
     5581 ttttctaatc taaattacaa ttaatataag aaataacata agagaaataa cataaaaaaa
     5641 gaaattttgt aaaaaaggaa ggatgtttat tgatgagttt agatgttatt tttatattga
     5701 aatcagtcat aatagctata gtagaggggc ttacagaatt tattccagtt tcatctacag
     5761 gtcatatgat tttggttggt aatttaatag attttaaggg gcaatttgct gaaatgtttg
     5821 aggttgtaat acaacttggg gctatattag cagtagtagt actttattgg aaaaagataa
     5881 aagatagcgt tatagaattt tttaaattta tatttactgg tggaaaagaa ggaaagattg
     5941 gttttagatt tggaatgaat gtaattatag gttgtatacc atttgcgatt ataggagttc
     6001 tattttacga taatataaaa tcattgttta atttacaatc tgtaattata ggatttatag
     6061 tgggtgggat tttattatta gtagtagaaa ctttgtttag aaaaaagaat cattccacag
     6121 ataatataga taaaattact ccaatacaag ctttaaaggt tgggacatta caagttttgt
     6181 ctgcatggcc aggtatgtca aggagtgcat ctactattat gggtggatgg atagcaggac
     6241 ttaattctcc tacagcagct gagttttctt tctttttagc agtaccagct atggtggctt
     6301 cttcaggaaa agatttattt gaatttgatt attcaataat gacaccaaca ttgtggattg
     6361 ctttggtagt tgggtttata gtagctttta ttgtatcaat aatagttatg gaaaaattcg
     6421 tgaatttttt aaagaaaaaa ccaatgagag tgtttgctgt atacagaata atcatgggtg
     6481 tagtacttgc agtattagct tttactaaca taattagtgt atagaaaaaa tagatttatt
     6541 atctataaaa attaaactaa gataaattat aattaacaat tatatatatg ttatttcaaa
     6601 aattaaatta ggaaggatag ttaaataatg aaaattttaa ctgtaaataa tttaagtaaa
     6661 gtatatggta aaaaaataat tttcaacgca ttaaatgaca taaattttag tatagaagat
     6721 ggagaatttg taggaattat gggtccatct ggtagtggaa aaactactct tttaaatatg
     6781 atttctacta ttgacaagcc aactacagga actatggaat taaagggcaa gaatccttta
     6841 ttgttaagag gtgaggaact tgcattattt agaagaagag agttaggttt tgtatttcag
     6901 gattttaatt tattggatac attaactata ggagaaaata tagtattgcc attgacatta
     6961 gataaggtca gtgtaaaaga gcaagatgag agattgaatg aagtatctac aatattaggt
     7021 ataaaagatt tattaggaaa aagaactttt gaagtatcag gtggtcaagc tcagaggact
     7081 gctattgcta gagcattgat taacaatcca tcaatacttt tagcagatga accaacaggt
     7141 aatttagatt caaagtcatc aaaggttgta atggagctat ttcaaaagat aaataaagaa
     7201 aataaagtta ctactatgat ggtaactcat gacccattag cagcgagcta ctgtagtaga
     7261 atcttattca taaaagatgg gtctatatat aatgaaatat ataagggtag tagtagagag
     7321 cagttttatc aagagataat ggatgtactt acattattgg gaggcgataa ctaatgaata
     7381 taaaacagtt tgctattaac aatatttcaa gaaatattaa agcgtattta ggatacttag
     7441 caagtattgt tatatcatct tcactgctat tttcttttat tatgtttaca agtcatccag
     7501 atttagatgt atctcaattt cctgattatt taaaagaagg tctcaagatg agtaagataa
     7561 ttgcatatct attcttattc ttctttgtgt tttattcagt tagtgtgttt cttaaaagta
     7621 gatataaaga atttggaata ctttatatta caggtatatc aaagcgccaa gtaatgaaat
     7681 taatctttat agaaaatatg ataataaata tagtatcttc tgtaattgga ataataatag
     7741 gcttaatctt tgcaaagata tttttagtgg ttatgtctac ttttttagaa ttatcaccat
     7801 taaaattcta tataccattt aatgcgatat tgtctacttt aatctatttt atgattttag
     7861 ctgtattaac atcaatattt acttctttta tagttaaaga aaatcaggtg ttgaagttac
     7921 ttaaaggtac aaaaactcca aaaccagagc ctaaaacatc actaatgctt gctatattaa
     7981 gtatttgttt gtttataata gcgtattata atgctattac ctcaaccgac caatatacaa
     8041 catatctgcg cttagttcca gttacatccc ttactatagt agcaacttac ttgttctttt
     8101 ctcaatttag tgtatttttt atgaagaagc taaaattaaa caagagattt tatagaaaaa
     8161 atacaaatat gctttggatt tcgaacttga tttataaggt aaaggataat gctagaatat
     8221 ttttcttgat aactataact tctgcagtgg catttactgc tataggtaca gtttattcct
     8281 tttggaaaga tgtagagcgt caaattaatt taatatatcc taacacaata tattattcaa
     8341 caatgacatt gcataatgac cctaaaaagc ctgatagcaa agaaaaagac aaggaaagaa
     8401 tgatgtttat agaaaataat ctaaaaaaag agaatgtaga ctatacaagg attaatggtg
     8461 aatttaaaac tgtatttccc aagaaaagtg attttaatgt cagaattatg aaagaatcta
     8521 agtacttaga aattacagaa actatagggg ttaagcctat aagttttgaa gatgatgaat
     8581 gtatatcatt attatcatca agtttaccag gaaataagaa cgtcaaagaa aatattattg
     8641 tagggaatag aggtttaaag gtggcaaaac aagtggaaga atgtgttatg ccagcgtatt
     8701 acaaaaatat gtatgtggta aaggataatt tttatgatag tatagaatca aaatttatag
     8761 tggatagatt tactgcattt gaagtagagg attcaagtaa aactatagag atatgcagag
     8821 aatttgaaaa taaatttaat aatgagtcag ggatgcaacc atatttgttt ttctctaaag
     8881 aattaatgat aggaactggg aaattaatgt attctacatt tatattttta gcaatattta
     8941 taggtttaat tttctttgtt actacaagta gttttttata taataaattt tatatggatt
     9001 gccaagttga taagaaaaag tatgaacagt taaataaatt gggtatgaca tataatgaga
     9061 ttaaaaaagc ttcaacaatt gagattggaa tagtattttt gtttccttat gtagtggctg
     9121 ttatacattc aatatttgca cttactgcac tgaaaaattc ttttgatata gaagtagcat
     9181 catcagctgt tttggtaatg ggaagcttgt tcttggttca aatagtttat tttttaattg
     9241 taagaaataa ttatttaaaa gagataaggt taaatttagt caatttttag ttactattat
     9301 taaatgataa taaaaagggt ttagaattaa ttaatattac tggaattata gatgtttatt
     9361 ttttcagtaa tattttttat tgtattaact attaaaattt tgtacaaata taaaatataa
     9421 tatggtaaat gtcaaaatta taaaaaaatt ctacttatat aaaatataaa gaggttatat
     9481 gtacatatgt gtagaaataa ataataaaaa tatcaattaa tgttattagg gggggttact
     9541 cattagtaaa ttagctaata cacttagaat gatgatatta attcaaagca gaggcaaaat
     9601 gaaatgcaaa gaattatcag aagagttgga agtaagtgaa aggcaaataa aaagttacaa
     9661 gatgtattta gagcaagctg gtatatttat aaattcaaca cctgggattt atggtggtta
     9721 tgaaatagat aaatgtaata gcatctcatt aattaaatta ttagatagtg aagtttcaat
     9781 ttttatatca actaagtggt agataagtag aatattagca atatcaacga gtatttagaa
     9841 acttatttta tattaataaa gcagtatata aaattgtaga atcaacaata gcatatacta
     9901 aaacatccac cttatattaa ctaaatcata tctaaataaa atacacttac ctacaaacat
     9961 tataaaatca atacaaaaat gaggtgaaac aaaatttatg ataaagaaat taaacagtaa
    10021 agacataaat aaaatcatgg aaatatggga aaaaagtaca atcaaagcac atgactttat
    10081 aagtaaagaa tactggcaaa ataactataa tactgttaaa aacgaatata tacctatatc
    10141 agatacattt gtatatgatg atggagatga aataaaagga tttataagca taatagataa
    10201 aagctttata ggagctttat ttataaagcc caaataccaa aatctaggta tcggaggtaa
    10261 acttttagat tatgcaacta aaaaatataa aagtctaagc ttagcagtat ataaagataa
    10321 taaaaaagca gttgtgtttt ataataaaaa aggttttaat atagtaaaag aacaagtaaa
    10381 tgaagattca ggatttaaag agtacataat ggaatatagt aaataatatg attacattct
    10441 attaaaagga taattaattt atttgccatt tgatttttta agagtaattt ttaaaataga
    10501 ctatatgaag ataaagatat ttattataga tgtaagttaa tcagaattga tatagctaca
    10561 aaagaggaaa ataaaattgt atggtgtatt tcagatttaa caaatttact atttaaagat
    10621 aataagtttg atataatatt aaagatactt tcacctataa actatagggt atttgaaaga
    10681 attttaaata attatggaat gctaataaag gtttgttaaa atttaataat tattagatta
    10741 tagaaaattg ccatgaaatt agttcatcaa gaactagttt catagcaatt ttaattctta
    10801 taagtgtatt tatttttaca gatttaaaaa acttatacag tatctaataa tgtaatctta
    10861 ttactttagc ctataaatat ttaaatctta aaatgttctc tttgagataa gtttatttca
    10921 gagtcacaat ttccacaaca gtaagatgtt gagacatctt taaatttttc agtattctca
    10981 tcaaagttca tttttttctt tgcaataatt cctaccttag ttaaattaga accacaatta
    11041 ttacagtttt gaattttgca taagttatat agtttatcaa gtgtaatatc ttctaataat
    11101 tcatcacaat tacaacatct gatagaagaa acttcactac acatttcaat aggttcagct
    11161 tctttagaag aaaatttata tgttatgtca tttgatactt tataacctgt atcaaatata
    11221 ttagaattac aacttggaca taagtacttt ggaagatata tatctttttt ttcaaaagta
    11281 tcactactaa tattacagtt taattcatct aaaattggtc tttcatctag gttattacct
    11341 ttatttaagc ttagaagata attataaatt tttttatctt cattctgttt tgatgtatct
    11401 aacaaatgct ccattatatt agtgtatttt ttgttttttg aaggcatgtt taaattcaga
    11461 taattgtttt caatagcaaa gcatactata ttgaaatagt tatatttaac tgcaaaaaat
    11521 aataacattt catcaaatac tttatctatt attttgtcag aaatacttat ccaaaaatct
    11581 ggggactcta agtaaacttt cctactaagt tcatctaact cttcatctat attattttca
    11641 ttaactttca tatcatcact tccttttaaa tatcttgtgt ataaactatt tgttactatt
    11701 tttt
//
LOCUS       DAEORZ010000132        11600 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEORZ010000132 DAEORZ010000000
VERSION     DAEORZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11600
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.133"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            226..1404
                     /locus_tag="KRH39_003025"
     CDS             226..1404
                     /locus_tag="KRH39_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8512912.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHHRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNKN
                     TTKEFIEKISKIMDNKISFSYGIVEVTSKSKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            1754..2635
                     /locus_tag="KRH39_003026"
     CDS             1754..2635
                     /locus_tag="KRH39_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8512913.1"
                     /translation="MPAYLFDSNSITNIYRVFSILLSFIVTGLYFSYKNHKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMIDINYELSKISNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            2826..2993
                     /locus_tag="KRH39_003027"
                     /pseudo
     CDS             2826..2993
                     /locus_tag="KRH39_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3253..5820)
                     /locus_tag="KRH39_003028"
     CDS             complement(3253..5820)
                     /locus_tag="KRH39_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG8512914.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQSFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVTNKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKKGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFISSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGMVTFDE"
     gene            complement(5823..6428)
                     /locus_tag="KRH39_003029"
     CDS             complement(5823..6428)
                     /locus_tag="KRH39_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8512915.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTNTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6802..8262)
                     /locus_tag="KRH39_003030"
     CDS             complement(6802..8262)
                     /locus_tag="KRH39_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG8512916.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKYEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVETLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8592..9170
                     /locus_tag="KRH39_003031"
     CDS             8592..9170
                     /locus_tag="KRH39_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8512917.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILNNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9461..10711
                     /locus_tag="KRH39_003032"
     CDS             9461..10711
                     /locus_tag="KRH39_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8512918.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQQQNSNIIKTIFTDIKDGIVHIC
                     KENGFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            10872..11441
                     /locus_tag="KRH39_003033"
     CDS             10872..11441
                     /locus_tag="KRH39_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG8512919.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         4002 a         1910 c         1641 g         4047 t
ORIGIN      
        1 atactttcca tacataatta ctatatttgg acacatagtt atcatacata ctgtatatta
       61 agatataata ctttttaaat tttatataac acagacacat gcaataaaaa ataaggtatc
      121 cataataagc tctttgcatg tacctaaata aaaactgtaa aatagaatat ataaaatcta
      181 atctatttta agtaaataca atttcacttt aaaagaggat gtactatgaa ataccatata
      241 tttttaatca taactactat attaataatc gaactatgta acatttacat atgcaaaata
      301 gcatcttttg gaaacaaaaa tataactata cataagaatt acaaatatat ttttttaata
      361 aaaccattaa ccttcttgat aataatattc acaaaattac cattaccact atattatata
      421 ttgttctaca tagtaagtca taaattgctc ttaagcagaa aaagttctac taacaaccat
      481 tttattccca atatggcagt aataagtttt tgtattttac accttattac tgtaagtaca
      541 atatcacttg ttaaaaatac aagtccagat ataatatttg caaatatgac ctataagtta
      601 attagtattt taatagcttc tattttagat tttatcttat ggtactctat gaataaaata
      661 aaagagcata tttatataat aactcaaaat caaatagagt tcagtctatt tttaaaattt
      721 atctggttca ctttaggata tatattattt gatagtatac aaattacatt tgctccacca
      781 agttctctaa cttcctactt tatattggga agtaacatat tattaatctt acaactaatt
      841 atattcatca atcatatata ttccattgaa aaaaatattt atttagaaaa agaacatcat
      901 agactaaaaa tgataaaaca agaacaatta ctaaaagtaa atttattaaa gaaaaaagct
      961 aacattgatg aattaacatc agcatataat cgaaaatttg cttttgaaaa aatacagcat
     1021 tacattgata acaatttatt attttcattg atatatatcg atttggataa attaaagacg
     1081 ataaatgata catatggaca tttagctggg gataactata ttattaactt ctcaagtata
     1141 gttattgaaa acttaggaat agatgacata tttgctagaa taggaggaga tgagtttata
     1201 gttattatgc ctaaatctaa taaaaacacc acaaaagaat ttatagagaa aataagtaaa
     1261 attatggata ataagatatc ttttagctat ggtattgtag aagttactag caaaagcaaa
     1321 atgactgtag aagaaattat taatattgct gatattagaa tgtacactaa taaacaaaat
     1381 aatcataaaa ggaacaataa ctaaaatgaa tatttacatg agcttacttg tagtctcact
     1441 atgtttttta tcttattatc tctatcatta taattttttt aaatatctag acataatgat
     1501 taataactct aaaaataaat acaaagtata cattgtatac tctcttttta attatttaat
     1561 ttttattatt tgtacttatt taaggttaca tctagttata aattggacaa tttttatgtt
     1621 agttttattt atgcaaatta aagtaactta caaactttcc acaataaaaa acctttttta
     1681 ttcgttaaac tgctccatca ttggtttagc aataaatata ttttttaggt ctttaatgtc
     1741 aatagtgtta aatatgccag catatttatt tgatagtaat tctattacaa atatttacag
     1801 agtattttct atattgctta gttttatagt tacaggatta tatttcagtt ataaaaatca
     1861 taaaaataat aagattaact ttgaggcagt tttagaggac aaaaaaaact taattttctt
     1921 attgagcctt atgtttggaa tgtacttgta tctatgtttg agtttgtcaa tatactatac
     1981 tgttggaaat gtattgattt taaaattata tggaatcaag tctgccataa tagttttggt
     2041 aggtaactat attgcagaaa gatatagctt tagaattact caaataaaag gttatgatat
     2101 taaaaatcaa atagaatatg tagaaagtgt aaagacagag tatgaaatta ataaattgaa
     2161 tgatattgta tatacagatt tactgacagg cttctacaat agaccttttg taaatcaaaa
     2221 gctatcagag tggacatcaa atcactacaa atttacctta tgttttactg atttaaatag
     2281 cttaaaacct gtaaatgata aatttggaca ccaatttggt gataaataca tatccactac
     2341 tgctcaaata ataaaaaaac ttataaatgt agaaaattcc ttactgttta gatatggtgg
     2401 agatgaattt ttattccttt tacagaatac aagttgtgta gaagcagaaa aaataatgat
     2461 tgatataaat tatgaattat caaaaataag taataccgac gagtatcctt atgctctttc
     2521 cataagctat ggattagttg agtgggatgg aatatccgat attgaaactt taatatcgaa
     2581 ggcagattct ttgatgtatg aaaataagct taaaaataaa tctactttaa actagctagt
     2641 tcagatattc tacaaaatat atattataag agagtactaa gaatgtcaaa atatatagat
     2701 tttgcaaata ttgaatacag tttactaatg aatgcactgg atgtaagtgt tagtaaacac
     2761 ctattagatg aacactttac tgttgtttgg gctaatgacc gttattatga aatgttcggc
     2821 tatacctagc taaaaagtta aaaatgacca ctgtagccga gggaatagag accagttttc
     2881 aaaaagattt cttgcaagaa ataaactgtg atatgttaca aggatacgtg ttctctagac
     2941 ctttaccaat aaaagacttt gaaaaactta tgtttcaaaa ctcctccaat taacaatata
     3001 aaattgctta ttataagtga tattattgta tcaacaaata aaggaattgt ctcaaaattg
     3061 attttttcta atcaaatttt tattttgaga cattctcatt tttttgcata agaaaatacc
     3121 caccaagcta cggtttttct taccgctgtt cggtgggtat tagtagtatt atgaaatctg
     3181 aaaactttaa gtattactac ttccgctaac gcaatcacaa aggtcgaaag cttcaaagcg
     3241 aaaataggct ttttactcat caaacgtcac catacctgcc aacccgtcca ccgttatcat
     3301 ttgtccgctc ttaatagttc gtgtggcaat gtgtgtagcc ataacggctg ggatgccata
     3361 ttcacgtgcg acaatgcttg aatggctaag aggaccacca atgtccgtta caatcgcact
     3421 tgcagatgaa aataatggtg tccatgctgg agttgtggta actgcaacca aaacacttcc
     3481 aggttgaaag ctttcaaaat ctgctggact attcagtaca caagctggag ccgtcacaac
     3541 accagtactg gtaccaatgc ccgtcaacac aattttccca tctttctgct tctgtggtgc
     3601 ttgtgatata gtttttttgt ttttttctgg caacttggat ggtgacacat aacccatgta
     3661 ttttttaagt tcagcttttc gaacaggtat tatcccgttc atatcagata gagacttgtt
     3721 tttatccaac tgctctataa gtacctccaa ttccgatttt gtgagccaat aaatatcatc
     3781 taaatgtgaa atagctcctc ctcgtatgaa tcgttctgag atttcctgaa acatacaacg
     3841 aatcagagga tgtcccattc ccatcaaata aatagcattt tcacgcatag gagaggtttc
     3901 ctgtgcccaa tggagcaact taaaaaatag ttttttacgc aatccaccta tgtgttgtaa
     3961 aatctcttct tctgcatgtt ttctacgctt ttcaaacgtg gtctggcgta aaaaaggact
     4021 ttcccctttt ccttccacaa atgtttttac ggattcaaaa gttggtgtaa gtgtttcctg
     4081 cggtgtggaa tgcgcaaaat caaactcata agcggtacga ccaaactctt taaaatactt
     4141 attgattcga tttttccatt ctatccatac ttcctgtgaa actccaacag gcacaactga
     4201 agatatgaaa tcttctgcta tctttattgc aggactgttt tgcagataaa ggttgatgct
     4261 gttattttgc tttacccatt ctgaaatacc ccacaactcc ttttccgatt gcagaacaat
     4321 cgtatcaaac ccaagcagga aaacggaagt atccgttata ccagcacgtc gaactgctcc
     4381 ttgaaataat tttgtaaata atgtttcact gatagaagca gcaggcaatg taagctgaat
     4441 ccttgtgaaa taaatacatg cggcataaaa tattgtctga ataccctcca taatttgatg
     4501 ggcgttcata gtatgcaggg gcttttcttc ccattgttta atagcatcct catatccttt
     4561 tcgtgccgtt tcccagcgtg caacgctgtt cataagtgtc ctacgtaaag agcgtgggga
     4621 aagggatttg atagcaatca aaagtggctt tgatttagca gaaagataaa catatccatt
     4681 aataaccgta tatgcccctt tttttggtaa taattttttc gcacttttac caaacatatc
     4741 tatccataaa agctctgatg cacggtttac gatttcaaga ccaagcgtgg cgaacaaagg
     4801 tgtaacagga tttggcaagt gttctgccaa acttcccttt gtgtatacaa catcctgttc
     4861 tggcaatacc cattctggtg gcagaacagt aattggacga gcctgaacaa tgtacaattt
     4921 atctttttcc agtgcccact ctacatccat aggcatttga taatacttct caattttctt
     4981 tccaagctgt atcagttgca ttacctgatt acgagtaaga gcatattttt ttctcaacct
     5041 ttcaggaacc atggtttctt ctgttccttc tgaagtacga accgtcataa tctctttatt
     5101 tgtcacttca tacgatataa ttcgttcgga gtccttatcc actacgattg tatcgggtgt
     5161 aactaagcta gaaaccaccg cttcaccaag cccccatgca gcgttaataa tcatttcgct
     5221 acgtctacca ttgattgggt ttaacgtaaa cataatacca gatgaatcgg aaaatgcaag
     5281 cttttgtact acaacagcaa gtgcgacaat ctcgtgcttt atattattct tcacacgata
     5341 agcaatagca cgagctgtcc ataaggaagc ccagcatctc tttacagcgt caataacttt
     5401 atcttcacct tgtatgttaa gataagtttc ctgctgacct gcaaaagaag cgtcaggtaa
     5461 atcttcagca gtcgcagacg aacggacagc caccgatatg ttccctaatc cagcataagc
     5521 cattttaatt gcgtccgaca cttcctgtgg catttctcca ttatggaaaa gctcccctat
     5581 tttcttagat acatcttcaa gttggctggt attattggaa tcaataccat ctagcagttt
     5641 attaattcga gactgaatat ggttcgtctc aacaaaactc tggtatgaag cagttgtaac
     5701 atgaaaaccg tctggcacag ggatgcctgc tgtcaatagt tttgataaag acatcccttt
     5761 tccaccaaca atttcaatgg ttgcttgttt atgttccagt ggcaagacat aataaatcat
     5821 aatcattcac ctccatcatt cataattccg tgcagaaata tattcattgt ttcatccaac
     5881 attttttccg aactatccga tggtcgtgac gtgtagctga ttgataacat ggagttaatt
     5941 aacaaacgtg tcacaagagc cgtattcgtt ttgcgaaaga tgcctgtggt cacaccatta
     6001 tcaattaccg attttataat atcttgatat gcataacgag catctttaag tctctttcga
     6061 tactcttctt ctaaataatc agattctgta tttaaccaca tcagtacagt tctatactgc
     6121 tctggaaatc taagatgatt aagcatgatt tttctaaggc actgttgtgg tgaaggttca
     6181 ccatcaataa tcctatgaac ttgttggata gttttttcta tttctttctc aactgcatat
     6241 acaacaatct cgtctttcgt tttgaaaaaa tcgtatagac ttgactttcc catgttggct
     6301 aaaactgcaa tttctctcat tgatgttttt tggaatccat tttcagccat gagactcaaa
     6361 gcaacagcaa ctattttttc tcggcgttct gcttgttgtt cgggtgtaag cacaattcct
     6421 tttggcatag ggcgatacct ccttttagcg accactggtc gtttcttttt tatttatcat
     6481 acagcgacca acggtcactt gtcaaggtta atttttttca ttgtatatgg tactcgagat
     6541 aaaaatgttc attttagaaa ttgctctatt taaatagaaa taggcttcat cattagaaaa
     6601 caagactata aatttgagat atttatcatg ttcaaatttc atcaatatca aagtttaaaa
     6661 tattaataaa aaaagaatta ctaagaaata acatataaag attcaaaaaa ggagttgaac
     6721 ttaaaattca attacaaaaa atcaaattta agacaactcc tttattcttt atctctacta
     6781 ttctttatct ctatttattt attacaactc cctaacattt accactactg ccttaggtct
     6841 agtcatagcc tcaattgaat acttaacacc ttgagttccc ataccagatg cctttacacc
     6901 taaaaatggg aagtggtctg gacctctttc agtcttatta tttatttgaa cagtaccaac
     6961 ttcaagttta tcagctatat aaaatgcctt atcaatatca cttgtaaata ctgatgattg
     7021 aagcccatac tctgattgat tagcaatttc aattgcttga tttatatctt taactcttat
     7081 aattggaagt actggaccaa atggttcttc ccaagcaatt ttcatatcaa catttacatt
     7141 atctaacaat gttggataga ataaattatt ttttctaaca ttgcctgtta ttaactttgc
     7201 tcctttatgt aatgcatcat caactaaccc ttgaacaaaa tcagttgctt tattatctat
     7261 aagaggagtg ataactactt catccattgg atttccaatt gtcaacgtct ctaccaatgg
     7321 ctttattttt ccgaccaatt tatccgccac actctcttgt actaaaattc tctttactgc
     7381 cgtacatctt tgtcctgaat atgaatacgc tccagatact atattcttag ctgcaaaatc
     7441 taagtctgca tcttctaata ctatagccgc atcttttcca ccaagctcca ataataatgg
     7501 taccattcca gatattttag atatatgctg accaacttca gtactaccag taaaattaat
     7561 aaaattaatt cctttatgcg ttacaacata atctcctatc tcactacccc tacctgtaat
     7621 agtattaagt actccatctg gtatcccagc ttttctcata atttctacca catgaagtgc
     7681 gctaatagcc ccttgtgtag caggttttaa tacgactgca tttccaccaa taagtgctgg
     7741 agcaatcttt gacattgata aatttacagg ataattaaaa ggagatatag ctaaaacagt
     7801 acctaatgga acccttgaaa cataagacat cttatttctt gttccaccag ggaaattatc
     7861 tccactaatg gcttcaccct ccatattttt tcccacatca gctgtgtatc ttaaaaaatc
     7921 tgctgttctc tcaacttctg cccttgcaga ttttatgtct ttggctattt caagtaccag
     7981 tatattagct atctcatcta tattttctaa caataaatca gcagccttat gaaatatatt
     8041 ggctctctta tatacaggca tttcagccca tacttttata ctctcttttg tgttttttat
     8101 aacctcatct acctcatatt ttgtcataga ttgtactttc ccaacaagag aattatctat
     8161 tggtgagtaa atttctattg gctttctcga attagattct acccattttc catttattaa
     8221 atttttatat acattttctt ttgttttaag ttcattaaac atatttttat tcctctctct
     8281 gatataactt tgtgtattta aattatagcc atttattcgt tatgtaaaca tttctttttt
     8341 tcataaacgt ttacctataa aaaaatttta agttaacgtt tttctacatt cttctatttt
     8401 aataacatac tagctccaaa tctatatagg tcacacttta atatcaatat gcgaatatac
     8461 caatatacca atataaatat tataaacata aatcacttga caaatttata agtagacaat
     8521 attatatatc tatgacttaa aatattttta aagtcataga tatcatatat agacagaaag
     8581 gattgataaa aatggctaaa agttttacag aaatagaaaa agacaatata agaaaaagac
     8641 taatttcaga atgtgaaatc aactggtcta aatatggata caaaaaaact aatattgatg
     8701 aactttgttc aaaatcagga attgcaaagg gttcattcta cacattcttt gattcaaaag
     8761 aaaagttatt ttttgaagta ttaaatggaa tccaaaacag acttatatca tcagtagatg
     8821 aaattttaaa taacatgcct ccaaaagaag gctttgcaaa atccttaaaa atgttatatc
     8881 gattatatga taaaaatcca tttttatatt caccaaataa tcctgatttc atcagcctaa
     8941 taaataaact acctcaagat atgtttttac aacttgagtc caataacata caaagttttt
     9001 acgacttaat tgagagacac aatctcaagt tgaagataga aaaagaaaaa gtttttggtg
     9061 tttgtaatgc tctactatca agtctattgg taaaagaaaa tattgaatat aactatattg
     9121 aagtgtttga ctttatggtg gataatcttg tagaacacat acttgaataa caatttaaaa
     9181 taatttttct aagtattttt ttatactaaa taggattaat atgattgaat taatgcaaaa
     9241 aataaacaat ataatttata aaataaatga taaactaaat atcacacaaa acttatagac
     9301 cctagagcaa acccattcca ctctactcta agccatctat aatagtttgt atatttctaa
     9361 taatattaga tattaaagta agaaaaaaat tccttataat aattgctatt atatcaatta
     9421 ttataaatat tttaataatc ttagataggt aggtacatat atggtaaaat cctcgaacac
     9481 ttttaataaa aacttcaact tgatgatatt aggtcaaata atctcattgt ttggtgcatc
     9541 aatacttaaa tttgctttat ctttatacat acttgatata actggtaaag cagaaatatt
     9601 tgctactata ctagcagtat cttcaatccc aattatagta ttctccccta ttggtggagc
     9661 tattgcggat atattcaaca gaagaaactt aatggttatt tttgattttt caagtagtct
     9721 tacagtacta atcttagcag tattcttatt caacaataat ggttcaatat tacttgtagg
     9781 aattattatg accatattat cagtcataag cacaatgtat caaccaactg ttcagtcaag
     9841 tacacccctt ttagttgata atgagcattt aatgaatgca aatggtattg tagctggagt
     9901 tgcatctctt acaaatattg caggacctgt attaggaggt gtcttgtatg gtgtgattgg
     9961 aattaatgca attatagtta taagttgtgt aagtttcttt ctatctgcaa ttatggagat
    10021 atttatacag attcctttta tcaaacaaca gcaaaacagc aacattataa aaactatatt
    10081 tactgatata aaggatggta ttgttcatat atgtaaagaa aatggattta tactaaaaat
    10141 acttttttta gctatagcaa taaacttatt tttaagctct atgttaatag ttggtatccc
    10201 ctatatagta aaaatcacta tgggagctag taacttctta tatggtctag cagaaggagc
    10261 aatttctttt agttcactgg tagctgctgt tagtattggt attttctcaa aacatctaaa
    10321 aatcactaat ttgcatattt cctttattat atgtgcagtt atgttcatac caatggcggt
    10381 ttcagtatat ccatttatat taaacacagg atttccagtt ccatttttaa tctttatagt
    10441 atcatctatg ctaatatttt ttacaacgac tcttatctca atatatgtta taacagtcac
    10501 acaattaaaa actccaaatg aactcttagg aaaagtaatg tcaattcttt ttgcaggaac
    10561 agcaattgca atgccccttg gtcaggttat gtatggtaaa ctttttgatt tattatcaga
    10621 tagagtgtac atcattatac taggagttgg atttattaca ttacttatag catttgtatc
    10681 aaagataaca ttgaggaacg aaaatcttta gatacaaaga atttagatat aaagatttaa
    10741 ttttcactaa tttctgaatt taaatgcaaa attgtgtatc agtatgttga cgttttagaa
    10801 attgattgat ataatcaatg gaaaataaac tttataaaaa ttcaaaatat tcagctagag
    10861 gagtgataaa aatgagttac atgccaactt tagaacaagc atgggaaata ttaagaaaat
    10921 acaataagga agaatttcat ataagacatg ctcaaattgt atctggagtt atgagatact
    10981 atgcaaagga gtacgaccct gaaagagtag aattttggga aatagttgga cttttacatg
    11041 atttagattt tgaaatgtat cctgatgaac attgcgttaa acaaagagaa ttaatgacag
    11101 agcttgattt agataaatca ataattgatt ctacaataag tcatggatat ggattaactg
    11161 gttcagatgt taaaccagag caatttatgg agaaagtatt atttgctgtg gatgaactta
    11221 ctggtttaat tggtgctgct gcaataatgc gtccttcaaa gagcgtttct gatttagaat
    11281 taaaatctgt taagaagaaa tttaaagata aatcatttgc tgctggctgt tctcgagacg
    11341 ttataagaag tggtgcagaa atgcttggat gggaacttga taagcttatc caaacaacta
    11401 tagaagctat gagaagcttg attccagaga tggcaatata gctcctccat acatatacca
    11461 tagaagttac atctttaaag agcatttctc tttttgatgc aacttctttt tatattttgt
    11521 agaataaaaa gtttaatatt ttaaactcac tatataatct ataataaata ctataaataa
    11581 tataatgtat aatagtcata
//
LOCUS       DAEORZ010000133        11595 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEORZ010000133 DAEORZ010000000
VERSION     DAEORZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.134"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            302..484
                     /locus_tag="KRH39_003034"
     CDS             302..484
                     /locus_tag="KRH39_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512920.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            1316..2230
                     /locus_tag="KRH39_003035"
     CDS             1316..2230
                     /locus_tag="KRH39_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBG8512921.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            2620..3267
                     /locus_tag="KRH39_003036"
     CDS             2620..3267
                     /locus_tag="KRH39_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG8512922.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(3434..4003)
                     /locus_tag="KRH39_003037"
     CDS             complement(3434..4003)
                     /locus_tag="KRH39_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512923.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNTTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESTMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(4008..4907)
                     /locus_tag="KRH39_003038"
     CDS             complement(4008..4907)
                     /locus_tag="KRH39_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8512924.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(4918..5067)
                     /locus_tag="KRH39_003039"
     CDS             complement(4918..5067)
                     /locus_tag="KRH39_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512925.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(5060..5644)
                     /locus_tag="KRH39_003040"
     CDS             complement(5060..5644)
                     /locus_tag="KRH39_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG8512926.1"
                     /translation="MKKFFMVVLGCIFVLGVISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            6065..7390
                     /gene="cdeA"
                     /locus_tag="KRH39_003041"
     CDS             6065..7390
                     /gene="cdeA"
                     /locus_tag="KRH39_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBG8512927.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     TFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKGRYKY"
     gene            complement(7485..8279)
                     /locus_tag="KRH39_003042"
     CDS             complement(7485..8279)
                     /locus_tag="KRH39_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512928.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTFISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(8280..9155)
                     /locus_tag="KRH39_003043"
     CDS             complement(8280..9155)
                     /locus_tag="KRH39_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512929.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEKELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(9159..9527)
                     /locus_tag="KRH39_003044"
     CDS             complement(9159..9527)
                     /locus_tag="KRH39_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8512930.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(9710..10369)
                     /gene="deoC"
                     /locus_tag="KRH39_003045"
     CDS             complement(9710..10369)
                     /gene="deoC"
                     /locus_tag="KRH39_003045"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBG8512931.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(10405..11592)
                     /gene="murJ"
                     /locus_tag="KRH39_003046"
     CDS             complement(10405..11592)
                     /gene="murJ"
                     /locus_tag="KRH39_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG8512932.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAKRKLH
                     LKK"
BASE COUNT         4087 a         1765 c         1287 g         4456 t
ORIGIN      
        1 ttttaaaatt aaattcctta aaagtatgat taattataat tgtataagta tattcttatt
       61 acacttatat taatttatat taaatatcta ctaactcaaa tattcataat taaactttaa
      121 actggataaa ttttctttta agaatgaaag tttatccagt tttgcgatta taataaccag
      181 ttttacatat ccacttgtaa aatatatcta agcaatatat catactatat agatttatga
      241 gtgaaataat atctgctatt tactgaatat ttttattttt cgttttttag ggggattagt
      301 tgtgtttaaa gataagattg atgaatgtgt tcatataatg actgcatata ttgctagttt
      361 aaaagaatac tattcattca tagaaactca aattggtgat tttattaaaa aatatggaga
      421 agatgttgta gagttatgtc tccatagagt gatgattcta ctatgcgaat gtggtttggc
      481 ataaaattaa atttattaaa atgggtaaga agaatcctat ataatattct tcttacccat
      541 tttaatatat caatttcata gcttatcgtt taaaatattt tacatccatt actagacaac
      601 tttacattct ctttttaatt ctagtatatt taaatcattt tagctgctcc ttatttattc
      661 ttattacttg ttttatttta taaataaaat aattcttttt aataatgttt tttgttgata
      721 ttcctatcac acatccaatt atttatcatt gtcttgaaat tgtttgcata cttaatttaa
      781 tccaattgaa acaccccaac atagaatccc ctgtaaaata gaagttggat taaatcctaa
      841 catctataca gaaaatacta ctccaagcac aagcaatata atggaatata tttatcactt
      901 aattttttat actttttaaa tcccatacct aaaacattaa gtgcagctat tagaattaat
      961 aattgttgtg gtataaaatt atgcttgctt catatttatt cattttttgc tataaaagtt
     1021 cttgttttac aaaaaacatt tgttttgtat caaaataata cttataaatt acataattct
     1081 cataaaaatt tctagtatta cttgtactct taataaataa tagccaacaa gtaccttaaa
     1141 tcattatatc tatatctaat aagaaacaca gtaagataat tttttcgccc ataacttaat
     1201 aaaaactgtt gatattagta gcaattgaat ttagttaatt tatttataat atacataaat
     1261 ttaaatgtta cgaataaagt attataaata ggatactttc taaagggagg aaattatgat
     1321 agataatcaa aaatatgtta ttttatcact agaattacat ttattttttt caagaattat
     1381 gaaagagcat gctctttttt tagaagcagg attcacaaat aaaaattata atcttgctat
     1441 ggaagctgac cactataaaa agcaatttga agatttatta tcatacactg ttagtgctag
     1501 taatggtata attagacctg atatattata ttcagaagaa cttgtaacta ctctcacatc
     1561 agttgcagaa caaaaaacag aagagtttac agggatagaa ataaacaaaa acatcactac
     1621 aagagaatta aatctacaaa gtggtgtaaa cccacaagtt ggtcaagatt tagtgaacta
     1681 tgtagctcaa cttaactctg atgcaataag attacttgat gggcttatta attttaaaga
     1741 aagagtctta gatggtgtac tatcatgtac tatatttaca tcaaactacc ctctacttct
     1801 tgaacatata atacatgaag caaatttata tcgttcttat gtagttgacc ttgaaaataa
     1861 aatagatatt gagtcaaaaa acgctaaaga aatagaatta ttctgggacc atattatgat
     1921 ggaacatgct ctgtttatga gaggattact agacccctca gaaggtgaac taataaatac
     1981 ttcaaatgat tttgctataa aatttaatga attaattgaa aaaacaaacg aaatgactga
     2041 ttctaatatc aagaacatta cagaagaaac tctaaatgaa actgttgagt ttaaagattt
     2101 taaagaagca ggagcatcag gaatagaaca gtgtaagata aaatctataa tattaccact
     2161 tttagcagac catgttttaa gagaggcaaa tcattatatt agaatattgg agagttataa
     2221 aaacatgtaa ttaaaaatta cttttaaaat aatctttgca tatttagata aaataaaggt
     2281 acagttaact gtacctttat tttttataaa cttataaaat ttaaacaagt tattcataaa
     2341 ataaaataat aatcatctat aacccacaac tttattttat attaaataaa aagtattagt
     2401 gattatgttt tataagtatt tctttattca gttatttatt aaaaactaat agtaaatctt
     2461 atactaatcg catatttatg ttataactat attgtaacta ttttcgtaat cttaggaaaa
     2521 gattacttat aaaattaacc ttatacaata atatatcgta taatcttaat tagcataata
     2581 gttacaatat taaaatttaa atatgggagt gattttagta tgataagaca aacaccatta
     2641 gagaaacgat atgacaagct aggtcctcat attgtaacag cactcaaaaa acgttatttt
     2701 gatgcttatt actgtaaaac tagaaatgaa gctttgcaac aaattttaga tttaattcct
     2761 caaaatcatg tagtatcatg gggaggatct gaaacactta aagaaatggg cgtacaaact
     2821 gctgttagag agaaaggatt taaagttatt gatagagatt tagcaaaaac tccagaagaa
     2881 agaactgaaa ttatgcgtca agcacttcta tgcgatactt ttttgatgag tagcaatgca
     2941 attagtgaag atggacaact ctttaatatt gatggaaatg gcaatcgtgt agctgcaatg
     3001 atatttggtc caaaaaatgt tatagttgta gctggtatga ataaaattgt aaaaaacatt
     3061 gatgatgcaa tacaaagaac tagaacaatt gcatctccag cagttgttca gcgttttcaa
     3121 gatgtcaata caccatgcta tattaatgga agttgtatag attgcacaag tcctgaaagc
     3181 atttgtgcat acatggttac tacaagaatc agtcgtccag caaaaaaaat aaaggtaata
     3241 ttggtaggag aaaatcttgg tctataaaat atacatacat gtaaatagct ctataaataa
     3301 taaaatcata tgaaataact tatttctaaa agtggtagaa taaaagcttc tacaagactt
     3361 tcattctacc acttttatat tcttttaatt tgtgtttcta tattattaat tgctccgatt
     3421 ttcatatata ttactaacta tttttatttt cataattttt ttgttcttca tcaagctgtt
     3481 tataataatc tttattatag tcagctagat aatctatacc acctcctgta atcacattta
     3541 ataatttcat tgtagattct tgattaactt tacaaacata attatcacca gatttaacaa
     3601 gctctaatgt aatattaaat tcttttttct ttgcagaatc aatatctttc atagttgtat
     3661 ttggcatcat ttctcctagt ttaggaggat tcttttttaa aatagaaacc atatcagtat
     3721 tgttaatttt taaatctaca gtcactttat ccttattatt ctttatatct gaaatttccc
     3781 aagtcatatt tttagaatac attttaaatc cttctttatc ttcgtatata agattaccta
     3841 accctgattt tgggaaatac ttatcaacag tatctgcatc ttgtgttttt attgaattaa
     3901 acatactatc cacttcattt tttatcttat tatcattagt acaaccagct aacaccagta
     3961 ctgatattgc aaacattata atacatataa taaatttctt cattaaatta tatcccccta
     4021 tacttttttg cacaaatatt attctctttt ttatcttttg aaaaagttgt agtaattgca
     4081 ttttgtggac aaacttttat acattcacca cacctaatac aatctggact atttacatct
     4141 ttatatattg gtatatcaag cttacatttt gtttgacatg ctgtacaatt tgtacattta
     4201 tctttatcaa ttttaaatct atatattgaa ataggattaa aaaaaccata gattgctcct
     4261 aaagggcata tatacttgca aaatggccta taagtaatga ttgacatcaa taatatcaat
     4321 agcaaaatac ttaacttcca actaaataaa aatcctattg attctcttaa attctcattt
     4381 cctaaaataa gtggtattcc tcccaaaagt gttcctgaag ggcaaatcca tttacaaaac
     4441 caaggatttc caccccctgc tacatttaca atagcaactg gcaaaataat tacaaacatt
     4501 attaagacta tatactttag atattttaga tatttatcac cataaacttt ttttatcttt
     4561 actggaaagg gtactttatg tagtaaatct tgtacaagtc caaagggaca aagccaacca
     4621 caaacaaatc tgccaaaaaa agcaccaaat gccattaaaa aaccaacaac ataaaatgag
     4681 aatttatact ctctgcttga taaaactgct tgtaatgaac ctattggaca tgctccaagt
     4741 gcacctggac atgaataaca attcaatcca ggtacacata attttttact ttctcctcca
     4801 tatatagttc cttctaaaaa tccatttaca tatccatttg ttaatgctgt aaatgcaact
     4861 tgagtaataa gtcttatatt attatttacc ttagataata acttcatccc tcttctccta
     4921 accaatacca atacactcaa gacaaatcat aattgcttta gttaatactg tatttgtttc
     4981 tccacgatat gtacctaatg caataaatat aatactaatt gcaataatag aaatgccttt
     5041 tttattatct ttaataaagt tactcattgg cttttaccat ttttagtctt tcatctataa
     5101 tttttttcca atcttcctta ctatttgcac ctacaacctt ctcacccaca aagtttcctt
     5161 tcttgtctac aaaataagtt gttggaaact ccataactct tttaaaatca gtttttttaa
     5221 gttcttcaga tacagtcaat tgttggtaag ttgcatctga ctttttcata atgtctttaa
     5281 ctaaattttt ctcttcattt gaaagaccag ttctcatatc agttttatca acttctacaa
     5341 ctaacccctt tatagctaca ttggaattat tactctcata ttcttttgat aatgcttcaa
     5401 gctctggcat ttcacccaca catggtccac accaagtagt ccagacatta ataagagtaa
     5461 gttctttatt tgaaaatatc tcttttgtta ccttatttcc atcaacatcc tccgtatcta
     5521 aattactaaa ccattcacta ttattcttgt ttttttctac tggcttagat tcttttttgc
     5581 tacatccaga aatcacacct aaaacaaaaa tacaccctaa aactaccata aaaaactttt
     5641 tcatatctca cactcctaaa atttatatag tatataaaat cgctgtttga aataaagata
     5701 acttctcttc tatattaaaa cattatattt aataaataag aactatttaa aataagttgt
     5761 ataaaagata tacagttaaa atctatttct tttcagacaa aatcttatat atatataata
     5821 acttataaaa cttaagatta tatacatata tttctaaaaa ttttcttaag aaacagttat
     5881 accttactaa tgcaatatac ctatttttac taatataagt ttattaatta gcactatact
     5941 aatttatctt aatatgattt tttgttgatt ttaagctgtt tatcgtataa ataaaatttt
     6001 tcttgactat atagttacta tataacttat tatatataga gagtaataaa aggaggtaaa
     6061 atttatggaa aatttattta caagaaaatt cactactttt gaatttctaa aatttgtttc
     6121 tcctgcaatt atatccatga tatttatatc tttgtataca ataatagatg gcatctttgt
     6181 atcgacatta gttggttctg atgctcttgc tagtataaat attgtactac ctataattaa
     6241 ccttgtttgt ggatttggaa taatgatggc aactggtgga ggtgctatcg tttctatacg
     6301 tatgggtgaa aatagacagg atgaagccaa ctctacattt tcttttatag ttttgttttc
     6361 attgattgtt gggattttat tcacagtaat ctcatatttc tttatcaaag aaatatctat
     6421 tttgcttggt gcaacagata agttattacc atattgtata acttatggta aagttatgat
     6481 tttatgtaca ccattttata ttttaaaatt tatatttgag tactttgcaa gaactgatgg
     6541 aaattctaaa tttagtttat ttctatcagt cattggtggt gtaacaaata taatattgga
     6601 ttatgtattt attaaatatt ttggaatggg tcttttagga gctgcagttg caactgctat
     6661 aggtattatt ttaacttgtg ttttaggtat tatttacttc ttatctaata aatctacact
     6721 aaaattaaga aaaccaaaaa ccgattttag acttataaga gatactatga taaacggttc
     6781 ttctgaaatg gttacagaat tatctacagg aattacaaca ttcttattta atgtagtagc
     6841 tttaaaatta gcaggagaaa atggacttgc tgctcttact atagtattgt atgctcattt
     6901 tttaatgaca tcagtctatc taggattcgc tgctggagtg tctccattaa taagctataa
     6961 ttttggtgct gaaaacagtg ataaattaaa agaaacattt aaacattctc taaaatttat
     7021 atttgtttct tctcttttag tgtttattat tgctttagta tttgcaccat ttattgttag
     7081 ggtctttgta agtccagata acacagtatt taaactagcc ttacaaggat taaaaatatt
     7141 tgcatttgct tttttgtttg ttggtataaa tatatttgca tcaggatttt ttacagcatt
     7201 tcacaatgga aaaatttcag ctattatatc ttttagtcgt acctttgttt ttataatcat
     7261 aggaatcata attcttcctc ctatgttaaa catgactgga ttatggctta cagttccatt
     7321 tgctgaggtt ataaccatat ttatatctat tctatttata aaaaaatata aaggtagata
     7381 taagtattaa aaattaatac ttaaaatcaa catttattaa aactaataaa cctgctttaa
     7441 aatacaaatt tacaaatttg atttttaagc aggttttatt ttagttataa taagtcattt
     7501 gcatactctt taattggtgc ttttctaagt aataaaatta taaatattat gcaaataact
     7561 ataattccta cctttgttcc aaataaaata aaaggattct gaaatgaata aataattttt
     7621 tctaaataca atagcatttt atgcatattt ttagttgcaa aattaactat ataaattacc
     7681 attaatatcg gtaatcctat caatatagta atactagtaa cttttctaac tctatatgta
     7741 aaagctccaa gtaacactcc tataaaacct atacatattt catttataca tatttgtaca
     7801 aaataattta aataagttag tggcatactt gatgttgaag gatctatcag tgctgatata
     7861 aatgtccaaa tcatatctct ctttagtaaa actgatagtt ctatactgta acctgttata
     7921 atttctataa ataacttcga taaaaaaatc aaaactatac ctaaaataga aatcacaaag
     7981 cttaaaataa ttaaatctat gacaatagct tttatacaac tttttctatc tgctcttata
     8041 cttaaagctc ctgaaaagtc tttatttgct atatttactc catacacaaa tagcagtata
     8101 gttatatatg ttaaaatagg attcacaatc gtggataaat tagttttaga tgcttctcca
     8161 aaaatcttga gaataaacac tggcaaaata ttcataacaa ttcccacaat cataaatata
     8221 attatataca atttagtatt tttattaaaa aattttatat atggctttaa ttcattcaat
     8281 tacaattcct ccttcttagt catgttaaca aacatatctt gtagccctat gtatcctaca
     8341 tcaatatttg aatttttaag tattttttca tcattttcac taaaatcacc atagtaaaaa
     8401 taagcaactg catttccaaa tgccttttta ggttttatat tttttataca ttctaatttt
     8461 tctaactctt ctttatttcc agtaatataa tgagcttttt gttttatgac atcaatttct
     8521 tcatctataa taatttttcc ctcatttaat attattacat gctccagtaa atcatctatt
     8581 tcatcaatca aatgagttga tattattatt gtccttggat tttttatata actatctaaa
     8641 attatgttat aaaactcttg tctatttact gcatccaaac caattgttgg ctcatcaaat
     8701 atggtgatag ctgaattaga acaaatacct attatatttg aaagtatagt tttcatacct
     8761 cttgacagtt gtttatactt ttttttagta tttaaatcaa aatatttgca taattctttt
     8821 tctaagcctt tatcataact tttataaaaa ctagaagaaa attcaaatat ctgacctact
     8881 ttaaaatctg gactataaaa ttctttttcc ctgacaatac aaacttcttc caaaactttt
     8941 atatcttctt ttgggttctt tcctaatatt tctatctcac cttcattttg aattaactga
     9001 tttaccatta tatttaataa tgttgttttt ccagctccat tcttacctaa taaaccatat
     9061 atcttatttt tttcaaagtt tagagacata ttattaaaaa tattttgctt cttaaaactg
     9121 tgagataaat tttctatctt aatagaacta ctcatatatt aatcctcctt gaaattaatt
     9181 ataatatcaa ccaattcttc tctatttatt tctaatttgt tagcttcttg aagtaggttt
     9241 tttacaaaat tatgtttaaa attttctttt ctagcttctt ttataatagt ttttgcacca
     9301 tctgatacaa acatccctat ccccctcttc ttatataaaa tatttttatc aaccaatata
     9361 tttatacctt ttaatactgt agctggatta attttataaa gctttgaaag ctctgttgtt
     9421 gatggaacct gttcttcttc ttttattcca tttgatagta tctcatcttc aattgctcgt
     9481 gctatctgaa taaatatagg ttcttcatta tttaatacta atttcatagg ttcctccttt
     9541 ctccattagt tagttactca actaattaac tatataactt ataatatctt gaaaaatatt
     9601 ttctgtcaat actaaataaa aaacagaggg aaaattccct ctgttttttg ttctttatat
     9661 ttataattaa attttaattt ttgtaatcct atcccataag ttaaaaactt taataatctg
     9721 ttgtatcttc attttttaat attttacata ttgagctagt tcctaacctt tctgctcctg
     9781 cctctataaa tttttctgca tcagaaattg atttaactcc ccctgcagct tttattttta
     9841 cattttttcc aacatgctct ttcataagct ttatatcttc taaagtagct ccacctgttc
     9901 ccattccagt agatgttttt ataaaatctg aacctgacat tgttactatt tcacacattt
     9961 ttattttttc atcttcatct aaataacatg tctctataat gacttttaat actttatctc
    10021 ctatagcttt ttttatttct tttatttctt ttcctatatt atcataatct ttatttttta
    10081 tatctgaaat atttacaacc atatcaactt catctgctcc attttcaata gcatcttttg
    10141 cttcaaatac ttttgtagca gtagtttgat atcccaaagg gaaccctatc actgtacata
    10201 tctttatttt accttttaga tattctgatg ctcttttcac atatgatggt ggtatgcaaa
    10261 ctgatgctac actcatatct acagcttcat cacataaaat tttaatatct tcccatgtag
    10321 ttgtagcttt taaaatagta tggtctactg tttttaatat atgtttcata aaatcctcct
    10381 aaatttataa ttaatataat tagattactt ttttaaatgc aattttctct ttgcaatgtt
    10441 tagtatcata tcaacctctt caactttaaa aataatcatt agtagtgtat aaattccacc
    10501 tccaactata actgaaatag ctagtgatac aaactcatta aatgttccta ctcccaaaat
    10561 tccaaatata aatttatacg taaagtatga taaaacaccc ataattaaag atgcaactac
    10621 cgatttcaaa gttgctttta ttattttatc ttgtccaaaa tatccaactt ttctctttag
    10681 gttcaagaag agtaacaata tacatgcaat tgatgatgaa ctagttgcaa gagcaagacc
    10741 accatgagcc attggtttta ttaaaactaa gtccaatact atatttaccc ctacagatat
    10801 aatcccattt accataggtg tttttgtgtc ttgtaatgaa taaaaaacct ttcccaaaat
    10861 atctcttaat ccaaatgcag tcataccaac tgcataaaaa attaaggcag ttgctgtcat
    10921 ttgggttgcc ctcgcatcaa aagcacctct ctcaaaaatg attctaacaa caggagttgc
    10981 aaaaaaaata gaagcaactg aaataggaat catagaaatt attatcatat ttatactaga
    11041 tttaactgaa ctagtaaact tcttttgatt attttctgca cttaacttag ataacatagg
    11101 atacataaca gatactattg aagctgtaaa tgtccctgta acaaaaccat ttaatctatc
    11161 tgcataagta agtgctggaa tgcttccttt aactaatgtt gaggctaaag ttgtatctac
    11221 caatgagttc acttgattaa cagcaacacc tataaatact ggtgaaagta atgctagtaa
    11281 tttaataagg ctatcatctt taaagtttaa gtagtataag tatttgtagt tcgttttttt
    11341 aacaaaaaac atgtaaaaga gcagttgaac taccatagca accacagcac caattggaag
    11401 tatgtatggt ccaaatatgg tacttaacat tattgaaata attattacta tattataggg
    11461 tatacttcca aatccaacta caataaagtt ctctttaatt tgtaaaaatg ctgacataac
    11521 acttgtaata cctatgaata ttattcctgt tctgaacctc ccatgactaa agtcacaggg
    11581 ttcctgcttc ataga
//
LOCUS       DAEORZ010000134        11291 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEORZ010000134 DAEORZ010000000
VERSION     DAEORZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.135"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(285..2015)
                     /gene="lpdA"
                     /locus_tag="KRH39_003047"
     CDS             complement(285..2015)
                     /gene="lpdA"
                     /locus_tag="KRH39_003047"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG8512933.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            complement(2182..3228)
                     /locus_tag="KRH39_003048"
     CDS             complement(2182..3228)
                     /locus_tag="KRH39_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBG8512934.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            complement(3241..4227)
                     /locus_tag="KRH39_003049"
     CDS             complement(3241..4227)
                     /locus_tag="KRH39_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBG8512935.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            complement(4252..5214)
                     /locus_tag="KRH39_003050"
     CDS             complement(4252..5214)
                     /locus_tag="KRH39_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBG8512936.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            complement(5252..6205)
                     /locus_tag="KRH39_003051"
     CDS             complement(5252..6205)
                     /locus_tag="KRH39_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBG8512937.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            complement(6650..8023)
                     /locus_tag="KRH39_003052"
     CDS             complement(6650..8023)
                     /locus_tag="KRH39_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8512938.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWAKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQDYIG
                     VIFSSAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            complement(8120..9766)
                     /locus_tag="KRH39_003053"
     CDS             complement(8120..9766)
                     /locus_tag="KRH39_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBG8512939.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMRWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            complement(9788..10954)
                     /locus_tag="KRH39_003054"
     CDS             complement(9788..10954)
                     /locus_tag="KRH39_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBG8512940.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
BASE COUNT         3391 a         2232 c         1201 g         4467 t
ORIGIN      
        1 ttttttatat tttacaaata atactttttt taaaaacaaa aaaatcactt ttcctaatta
       61 caattgaaat cattctaaaa tgataatgtt attttatatt actatctttt ttgtacaaca
      121 aaaactacaa ggattctata ttctatattt ttccataaaa aatacccctt attatgcaaa
      181 taaattattt gcataataag gggtatggct aagtaagtgt tttaaaattt tatgatataa
      241 attaatatta ataatagact taaattaata atttatgttt agatttattt tttaggtaag
      301 tgtaaacatt ctccatttac atctgccaaa gcttccatta aagcttctga agtacatgga
      361 tgaccaaata tcaactcact tgcttcatca gtaggtatct ctaacgcttt aaatgatgct
      421 gcatgattta taagttctgc tacaccacat ccaaacatat gtatacctaa aatttctcca
      481 tatttagcat ctgctactac cttcacatat ccttgttctt gaccagaagc taaagctctt
      541 ccatttccag caaaattaaa cttaccaact tttacattat atttttttct agcatcctct
      601 tctgtaatac caactgatgc tacttctgga attgtataaa cacaactagg taatgcacct
      661 aagtcaactt ctttattcac acctagagca tttgaagctg ctacctctcc cattttaaaa
      721 gcagcatgag ctaacattac acctccagta acatcaccta ctgcatatat gcttggtata
      781 cttgtttcca ttttactatt tacaactatt gaacctttat ctattttaat atctaagtcc
      841 tctatgccag aaagattagc ttctcttcct atagcatata gacataaatc tgcttttatt
      901 ggctcttcac cttcaatgca cactaatatg ttatttcctt cttctttaaa ttcagaaact
      961 ttctttttag ttaatacatt tatacctttt ttactaagag aatattttaa agattcgctt
     1021 aactctttat ccattcttgg gattactcta tcttccattt ctactatagt tactttagaa
     1081 cctcttgaat tgaatatctc tgcaaattca caaccaatca ctccaccacc aattattact
     1141 aattcttctg gcactgtttc caaatctaat gcttctgtac tagttattat taaatttgat
     1201 tctattcctt ttataggtaa tatccttact ttggaacctg tagcaattat tatattttct
     1261 gtatctaaaa cttttccatc tgataaaatt actttatgct cttctttaac actagcttta
     1321 agattaaaca catctacatc tctacttttt aataatccgc caacaccagc tgtaagtttt
     1381 ttaactactc tatttttata ttttatggcc ttcttaatgt cctgttcttt atctacagtt
     1441 acttttacac ctctctttga taattggtca atttcttcta atatttctgc tgtttttata
     1501 taagtctttg tagggataca acctctattt aagcaagtac ctcctagtat attttcttca
     1561 actaaagcaa ctcttccacc taaaagagct gcttttaatg ctgataaata tcccccaggt
     1621 cctcctccta ttaccactac atcataatca tgattacact cttttttgtt tattttttct
     1681 tctttaaatt ctatattttg attctcttca actatatctt cttttgaaac atttccagat
     1741 aaatatttag cttttacttc ctcttgattt tccccttttt ctgctattac acctattact
     1801 gtaaatactg gtaatacttc accttcctct ttatgtatga ttacagcaag tgtaccttct
     1861 ccttctgact ctatctccat attaacttta tctgttgtta tttctagtat aggttctcca
     1921 atctttactt cttcgccttc ttgctttagc caagatacta tagtaccttc ttccatggct
     1981 actcctgctt ttggcattat tacttctact gacataggct ctcctcctat ttattttcat
     2041 atattttaat taagcaaatc atattcacat atagttactg ttaaaaataa ttcttaatat
     2101 ataaaagctt taaatttact gaaacaatta taataatata atctattcaa actatttata
     2161 tcagtaacaa aatatcaaaa cctatacaag cattgataaa ggattttcca acaattcttt
     2221 taaatctttt aagaattttg cagctactgt tccatctatt actctatggt ctgatgttaa
     2281 tgataagttc attataggtt ttattttagc ctctccattt actggaacaa acttatcttg
     2341 tgtagcacct acccctaaga ttgcacttga aggcatattt attataggtg taaatgtagt
     2401 tataccatac attccaacat tacttattgt aaatgtatta ccttcttggt ctgctggcat
     2461 aagtttccct gtctttactt tttccgcaag ttctttgctt tcttttgcta tttcttttaa
     2521 agactttttg tttgcatttt ttactaccgg aacatataag ccttcatcta aaccaactgc
     2581 aatagctata tttacatctt tatatctaaa tataccctca tctgtccatg ctgaatttaa
     2641 ggcttgatga tttggaagta ttttagatac tgccattact attaaatctg tcatagttaa
     2701 ttttactcca gttgattctt ttactgtgtc gattaacttt gctctaagta ccttcaattc
     2761 tgtagcatct acttctatat tgaatgtaaa tacaggagca ctaaaataac tttctgacat
     2821 tcgttttgct acagtagctc tcattggatt tggcttttca aatattcctt ctactgtgtt
     2881 aaacattctt gaattttcat tttcaatttt cagctctttt ttctcactag atttagatgt
     2941 attagttgat acaatagttt cttttccagt taatttttcc acatcacact ttcttatctt
     3001 tccattatat ccagtaccta caatagtttc taaatctata tttaaatctt ctgctattct
     3061 tctagctaaa ggagtagtat ttgctttatt atcttttaaa taatttaaca catctactaa
     3121 atgaatcctt ccattttctc cacttccaat cagcctatct aattttatat tgtcttttct
     3181 tgcctgactt ctagctgctg gtgtggcttt tattttattt gccaccatat ctatccctcc
     3241 ttatctaaca attaaagatt ttattgcttc ttctatttca tcaactcttg ggacaactgc
     3301 tttttctaac tctggattat atggtattgg cacatctttt ccacaaattc tttttactgg
     3361 agcatctaaa taatcaaatg attcactttc tgttatcagg gctgatattt ctccacctag
     3421 cccaccagtc ttagctgctt catggcaaat taaaactctc ccagttttac atacactctt
     3481 tactatagtc tctttatcta atggatataa agttcttaaa tctataattt caacatttat
     3541 attttctttt gatagagtct ctgctgcttc ttcaacactt tgtaacattc ttccataagt
     3601 aattactgta acatcagtac cctctctttt aatatctgcc tttccaattt caattacata
     3661 atcatcttct ggcacaaaac ctttttttct gtataacaat ttattctcaa caaatatgac
     3721 tggattatta tctcttatag ctgctttcaa caatccctta gcatctgctg gtgtagaagg
     3781 tgcaactact tttactcctg gtacatgaca aaaccaagct tctaaacttt gtgaatgttg
     3841 ctctgctgaa ccagttccag aacctcctgg gcatctaaca accattggaa cttgtgcttt
     3901 tcctccaaac atatatctca tttttgccgc ttgatttaca attgcatcca tagaaattgt
     3961 tacaaagtcc ataaacatta cttccataat aggtcttaag ccagttgctg ctgcacctgc
     4021 tgccgctcct gctatagctg cttctgatat aggagtatcc cttactcttt ctggtccaaa
     4081 ttcatctatc ataccaactg atacaccaaa tgctcctcca tatattccta tatcttctcc
     4141 cataaatatt acattttcat ctcttctcat ttcttctgac attgcttcct ttattgcttg
     4201 tgcatatgtt aattcccttg tactcatttt tattttcctc ctttaccatt cttagtctgc
     4261 atatacatcc tctaataaag actctatttt tggatttggg ctattttgag caaactctac
     4321 tgcatcttct attgattgct ttgcaaattc ttgaatctta tctaattcat cttcattaga
     4381 tagattattt tctataagat aattttttaa aaattcaata gggtcttttg ctttccatga
     4441 ttctatttct tcttttgttc tatatacatt tgcatctgat tttgaatgac ctaaccatct
     4501 ataagttcta ctttcaatta atacaggtcc ttctcctctt ctacacttct cagcagcttt
     4561 ttgtactgtt tcgtatactt ctattgggtt atatccatct attgatattc cttctattcc
     4621 ataagaagct gctctagtgg caatactttc tatattcata tgacgtttta tgcttgttga
     4681 cataccgtat aaattattct cacagtaaaa tattattggc aatttccata ttgaagataa
     4741 atttattcct tcatgaaaag taccttcatt tgatgctcca tcaccaaaac tacataaaac
     4801 tattttgcct gtttttttat actgttgtgt aagtgcagct cctggagcta ttgtaagacc
     4861 tccacctaca actccatttg ctcctaagtt acctgactca atatctgcta tatgcattga
     4921 gcctcctttt cccttacaaa atccagtttc tttacccata agttcagcca tcattttatt
     4981 taggtctatt cccattccta taaattgact atgtcctcta tgagttaatg atactaaatc
     5041 tcctttttct aaagccatta cagccgccac acttgacgct tcttgaccta cagataagtg
     5101 agttgttcca tgaaccatac ctcttgcaaa aaaccaacta actttctctt caaattttct
     5161 agcctgattc attctcttat acatttcaag caatgtttct tttgatattg acatatacat
     5221 tccctcctgt taacttttat ctatctttct attagtcaca atcttctaat aatttaagtg
     5281 ctaaactgtg attaattgct aaactattta taaatttcgc ttttaatgat gcctttattg
     5341 cctctatttt ttcatctcct gaagcgacac ctattgttct ttttatcttt tttaaatctt
     5401 ccagtttcac tcctaatact ctttgattaa attcaagtat agaagttttt ccttctttat
     5461 caaaggcttg taaacaaatt gcacctattg agttgtattt ttttaaatct tctatatctg
     5521 cttcattcat gtatccagaa gccattattg ttgagtttaa actcatagga tttcctatac
     5581 ccacaattgc tatagttgtt ttatctatta aatctagcac ttctttaatt ttttcgtctt
     5641 tgcaaagttt ctcttttgta gacaagtcag acataacagc tggagcatgt aataatttaa
     5701 aatcacctcc aaaagccctc gctaaactta cggctatttg atttgcatga atatctattt
     5761 cattgtctcc aatccctcct aatagaggta taaatgtaac atttttacag ttactttttt
     5821 ccacatatct tggtatttct tttagtgttg ttcctattga aacacctatt atatcatttt
     5881 cctttactac tctagttaaa tattcagata cagctcttgc tagttcttgt ttttgtgtta
     5941 gtgcatcctg tttatcatct actactatta cttcttttaa tttatatttt ctctccaaac
     6001 ttctctctat tttttggtaa tcatttttca aaacatcaac tatctgtatt tttactattc
     6061 cttgttcaaa agcttctttt aatattcttg atattgttgg tcttgatatt cccaattgat
     6121 ttgctatttc ctgttgattc aaattatctt cgtaataaag tgaacaacac ttcatcatta
     6181 atcttaagtc tccaattttt ttcatttgct ttcctccaaa atcaagcttt tcaattatct
     6241 ttacatttgt tcaatcattt tacttaattt cagtaacatt atagaagggg ttcatataaa
     6301 aagtcaactg tttttttgta taacaaaaaa aaggtgatac ttttcttgta ccaccacctt
     6361 cataaatagt tgaaaaattg cttcttagtg catattatat attacttcta attgttaaat
     6421 atcatctcca taaattaaaa attaattgtc cacttataat gctttaaata acaataactt
     6481 attaaatcat ttctaaaaaa ccaaccattt catcatttaa agtcttctta tatttttatt
     6541 attcgcttct ctaaaaattc ttcaataatc ataaatatat aatagttttt atagggaaca
     6601 tttacaatag accttaatta aacttaagac tttataagaa actttttcat tatttagtat
     6661 ttaattcatt aagtatagta ctatattctt tatcaagctt ataaaaagac attatgataa
     6721 tttgtaatat tgttataaat ataggtaggt atataaaaca tacttttatt gatactatag
     6781 ctaaatcact ttgagtagct gatgttccta cataaccacc aattgataat actgcaccta
     6841 atattgctga acctaatcca tttcccactt taaacccaaa actagatgca ctatttataa
     6901 gcccctcagt tctatatcca gtcttccatt ctccatattc aatagtatct gaaacaatag
     6961 caaacattgt tgatgctatt ccagcacttc ctattccctt tatagcactt gaaaatatta
     7021 ctcctatata gtcttgtcca actattataa acataacatt tgacgatatt aatattattg
     7081 aacctactat taatacattt ctttttccaa atttttttat aaaaggagca accataaaca
     7141 tagctataat ttgggatata tttgaaacca tatttatagg accaaataat ttttcatctc
     7201 ccaaaataaa ctttgcataa tatacggatg aaccaccatt tatcgcaaat acaataaata
     7261 tgcaaactaa acataatgtt atttgtatcc agtacttatt tctaaataaa gatttaatac
     7321 ctattttaaa tggtattact tcattcctat cttgcttaac tgcttttact ctttccttgg
     7381 ttccagtaaa tgttataaaa aataccatta tagatgctat tccaaataca aagaatgttt
     7441 tagcccatgc tgataaatta tttccaaagt attcaactag aggtagtgta agtccattta
     7501 ttagtaaggt accacaagtg gctaatatca ttctaaatat acttaatact cctctttcat
     7561 actggttttg agttataagt gaatttagag ttgcataggg tacattgatt gctgtataaa
     7621 taacagtaga aactaagtta tatgtaataa aaatataaat taatttagaa gtgataccaa
     7681 gatttgatgg aacagaaaat aataatactg ctgctatagc aaaaggaatc gccattctca
     7741 aaatccaagg cctagcctta ccgtatttgg atttagttct atccactata attcccattg
     7801 ttaagtcact aaacccatcc agtatcctag atacaagcat aatactacct acagctgctg
     7861 cactaacatc tgcataatta gtataataaa acagcaaaaa tgctgacata gctgagtata
     7921 tgatattgca ccccaaatcc ccacaaccat aagaaattct ttcaaaaaat gaaatctttt
     7981 catatctaat agtagttgct ttaacattaa cttctgtatt cataatacca tactcctttt
     8041 tgtaagattt gccttctaat gtttcttatt tcagagcata agatttacct ttgctctgaa
     8101 aattttagta atgattttat taatcgacat cttttgattc atctactata tattcaaaaa
     8161 aatcaaaatc agcatagtgt ttatgcttta cacgatctgc acaagttaac cctataaacg
     8221 tacctgtaaa ttctccatat ttacagtact catctgaaaa cttagttgta tcaaatacct
     8281 ttcctattct ctggtagttt ttttcatcat aactccattc aaaatatgac ttacgacctt
     8341 gtatatacag acgaagatat attggtatat cttttattgg tatcctagta tttaaaaatt
     8401 ccgtcttctc tccattttca agatgtatta ttgatagtgc actttgaccc aatgtttcac
     8461 tataatactt tcttaaattt atataattca tattatcata atacattatc aaacccgcac
     8521 tatgctgatg tacctctggg taaaactcca tcttagtggt tattcttgca tatactgaag
     8581 ttaattttct tgccaatatg cttactttat ttaatgatgt tcttgattct tgtcctctaa
     8641 tccttacata tcctggtcta gacttaacat ctgcaaatga taatggtgaa atcctaggag
     8701 catagtactg taaacctaat tcatttgaat caaagtcatc aaaacttggt atagatgaaa
     8761 ctaaatgttc ttctagttta ctttctgata cgtattcttt tgccaaatta ctttcatctt
     8821 ccattcgaag ccaattatcc tttgtccatc tcattttttg aatagctgtt tctctaccta
     8881 aagtacacct taactctggt acaaatggtc tagatgttag atgaactaaa tatacttcac
     8941 ctgatgttgt ttctatatag cttccatgac cagatttttg aagttttgat tctgggttat
     9001 agtatttagg ctttagatgg tcagggtcat gtctttcata aaaatcgcct ggaattgaag
     9061 ttacaattgg attcattgaa tctttttcat aaggtcccca tatattttta gcccttccca
     9121 tagttacact atgaccatat ccagttccac cttctgcaca cattatataa tagtaatctc
     9181 cacgctttgt tatatgtggt gcttcaatac aacctctatc agttccccct gaccatattc
     9241 tctttggata tcctactatt tctttctttt ttgtacagta ttccactaaa catataacac
     9301 caggtttttc atatccttct ctagtctccc agtctaatga tgcaatccac tttttaccat
     9361 catcatcatg aaaaatagac gcatcaaaac ctgatgagtg tagatatact ggttcactcc
     9421 actctccctt tatatctttt gatgtaatta aataattatc aacatcaaaa tatcttgcat
     9481 tcatagagtt cataattcca taaactatgt aaaataaatc ttcttcttca caataagtca
     9541 aacaaggtgc ccaaattcct ttagatgaag gaagcttctt caaatctatt tttgtttcat
     9601 ctgtcaaaat atgagtatat aactcccaat tttttaagtc tttagagtga taaacaggta
     9661 tacctggaaa ccattcaaaa gatgaaacta caagataata atcatctccc tttctacaaa
     9721 tacaaggatc tggattaaaa cctggaagta tagggttttt aatcatatct tttcctccaa
     9781 ttttagttta atattctata ggataccatt cttgagctac acatttgcct aggtcccaac
     9841 tatcccatcc tatccaacct tcttcattaa ctgtatctag taataactta taattccaat
     9901 agtagtgacc acttccattt ctccatgcgt caagctgtgc ttttgctatc ttcctatata
     9961 attctctttt atcgtctttt gataatttat ctatatttga ttcaatccca tttagaggtg
    10021 attgacctcc atttgtatct ataccacaag catatgaatt aaataagctc cactccccac
    10081 atataacagg aaaatatttt tgcatttgta aaatatcttt agcataattt tctctaatat
    10141 atttgagata gctatcaata ctttgttcac atccatctgc ttcagctaac atcaaatatt
    10201 ggtgtgtatc tagaacaaca tttttaaatt cttcctctct cataaagtct ttccatgctt
    10261 ttaattcaaa tgcatcatga aacactataa cgttttcctc tctcatataa gggcgcatta
    10321 ccttatatgc ttttatataa aaatcacgta aaaactctaa tgaattaggt ttactccccc
    10381 ttgccatctc cttatctaca gccttaaacc gatttggaac attcattatg ttccacattc
    10441 tttctgttat aggctcattt aaaatttgga ttccatacaa ttcatgcctc atcccataac
    10501 gttttgcaag tctttctaat acatttaatg taaacgctac atattctgga ttttgtgacc
    10561 agctacatat acctgatatt cctccattat caaatccatt ttggcttcct ggaactgtat
    10621 gtaagtctat caatatacta agcccatact tatctgccca agcaaatgct ttatcaagtt
    10681 ctttaacaca tcctatgaat ggttcacaat ctccaaaaat aaagtatggt actggtatac
    10741 gaactgaatt aaatcccata gatttaatcg ttgcaaaatc tctctctgtg atgtattcac
    10801 tacgatgagt cttaattcta gattcatatg cctctcttga aagctgtctt ggtaaatagt
    10861 attcatcttc agcttctgtg ccttcaaata aacttgaggt catccatttt tctaaaacta
    10921 accaacctcc taaatttaca ccttttatat acacataaaa tcctcccctc ttatcttatt
    10981 taaataggta tattttgcac ttataataaa ataatttttt taagttattt taatatacag
    11041 ttattatatt tgattttatc cgttttctta agtatgttta ttatctaatt tattagtcta
    11101 tattatcttt ttaatattga aaattataaa tttactattt ataatttaaa attataagga
    11161 ggatattatt atgaacagaa ataatttttc acatgaaata attgaaccta ttgataattt
    11221 agatgttaat ttcaaactat ttgattaaaa aatagctacg cacactcgtg actttagtca
    11281 tgagttagta g
//
LOCUS       DAEORZ010000135        11269 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEORZ010000135 DAEORZ010000000
VERSION     DAEORZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.136"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(52..879)
                     /locus_tag="KRH39_003055"
     CDS             complement(52..879)
                     /locus_tag="KRH39_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG8512941.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            1261..1581
                     /gene="sugE"
                     /locus_tag="KRH39_003056"
     CDS             1261..1581
                     /gene="sugE"
                     /locus_tag="KRH39_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBG8512942.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIVTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            complement(1714..2394)
                     /locus_tag="KRH39_003057"
     CDS             complement(1714..2394)
                     /locus_tag="KRH39_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG8512943.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            2790..3842
                     /locus_tag="KRH39_003058"
     CDS             2790..3842
                     /locus_tag="KRH39_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG8512944.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGYGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            3889..4305
                     /locus_tag="KRH39_003059"
     CDS             3889..4305
                     /locus_tag="KRH39_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8512945.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            4334..4807
                     /locus_tag="KRH39_003060"
     CDS             4334..4807
                     /locus_tag="KRH39_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8512946.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            4854..5612
                     /locus_tag="KRH39_003061"
     CDS             4854..5612
                     /locus_tag="KRH39_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8512947.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            5636..6475
                     /locus_tag="KRH39_003062"
     CDS             5636..6475
                     /locus_tag="KRH39_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG8512948.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMCILIVVGAITGIL
                     GLF"
     gene            7012..8151
                     /locus_tag="KRH39_003063"
     CDS             7012..8151
                     /locus_tag="KRH39_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG8512949.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKIIEDKNDLALESYYSSVIEGAFSNRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HIGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKEFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICSKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            8177..8365
                     /locus_tag="KRH39_003064"
     CDS             8177..8365
                     /locus_tag="KRH39_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512950.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
     gene            9149..10933
                     /locus_tag="KRH39_003065"
                     /pseudo
     CDS             9149..10933
                     /locus_tag="KRH39_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT         4353 a         1299 c         1961 g         3656 t
ORIGIN      
        1 ccataaattt ttttaacact taatttatga tttatggggt tatgttctgt tttatttcaa
       61 agtattaagt tcatataaat cagttcttct gtgcttaaga agtggtaact gttgtcttat
      121 gtcaggaatt aaatctctat caatgtcagc ataaataata cattcttttt catcagcttg
      181 agcaattatt cttccccaag ggtctgaaat tcttgaattt ccaaaagcta tataagaagc
      241 attcatattt ctagcaggtg cagcaccaac atagaatatc tgattatcta aagcacgcat
      301 tcttatagat aactcccaat gggcgggacc agtagtcata ttaaatgcag caggtaagat
      361 aactatttca gcacctttaa gagccattaa acgtgataac tcagggaaac gaatatcata
      421 gcaaatagct ataccaactt ttccatattc ggtatctatt acagtgacat catttccagc
      481 tgtaagagta tctgattctt taaaactaac ttttcctgta acttcaatgt caaataagtg
      541 cattttacga tgttttccaa ttagagctcc atttttatca aatacatagc aagtattata
      601 gatttttcca tcttctaact caggaatact accagaaact agataaattc catgtttctc
      661 agctaattta cttaatatcg ttgttgtttc acctggatat tcttcagcaa atttaggaaa
      721 atatttattt tcatatgggc agttaaacat ttctggcaaa actacaattt tagctccatt
      781 gatggcagct tctgttacca tatctacagc atgattgata ttttcaattt tattttctgt
      841 agtaatcatc tgacatactg caattttata gaaattcata gaaattactc ctttcaatat
      901 ttttatgtta ttataacata ttaaataaaa attagatatt cttagattga cccaaatggg
      961 tatggagaga agtgaataag aatattaaaa ggtatataaa agttattttt attatagatg
     1021 gaacccatat attgactttt tttatatatg ttgatagtat tattatagat aaaatagaat
     1081 aattgataga gttttatagg gttccgcaat tattaatgat tggtctggtc cgagataaaa
     1141 cccacaacat agtttttgtt gtgaacacgg aaggacaaaa acctgggaga tattttataa
     1201 tatttctcag gttattttat tggaaaaata agagatatat ttgaaaagag ggatgagttt
     1261 atgaaatggt tatttttgtt tgttgcaggt ttgtttgaag tgttttgggc ttttcaacta
     1321 aaaaattcac atggattttc taaattaact ccaagtatac ttactgttgt tggaatgatt
     1381 gtaagttttt attttttgtc attagcacta aaacatcttc cacttggaac ttcatatgca
     1441 atatggactg gaattgttac cgtcggtaca gctattgtag gcatggctat tttaaatgag
     1501 cctattagta tatcaagaat tgcatgtata ggttttattg tggtgggaat tgtagggtta
     1561 aagctattat ctactaatta gagtatatta atttaaataa gctatgaaat taaaggcgag
     1621 aaaatgttta aaaataatca acatttaaaa agtctttact tcatagctta tcttatttat
     1681 aagtttatta tatttgtaaa ttatatgtat atactattta tctaataaaa gagcagttaa
     1741 atccataggg tcaactatta caccatcttt ataaacacgc atatttccag atgctatttc
     1801 atctattaag attacttcat cattgtagaa tccaaattca aatttaatat cataaagctc
     1861 aagacctttt tcagctatag tatcacgaac tatgttagaa attttttgag tcatgctttt
     1921 cattgaatca aattgagctt gagtcataat gtttaaagct tctaaacctt cagatgttat
     1981 aagagggtca caacggtcat catcttttaa agttgcttct acgtaacaat ctagtttagc
     2041 accttcttca acacatgaac cataacgacg taagaaacta ccaacagctc tataacgaca
     2101 aataacttct aatccattac caaaaacttt tgctggtaga acttccatag ttgcttcttc
     2161 taaattagca cttacataat gagttttaat tccagcattg tttagaattt caaagaactt
     2221 aacagatgtc tctaaattag ctctaccaat tccatctata cttagaccta cactattttc
     2281 gcctgggtca aatactccat ctttaccagt tacatcgtct ttaaatttaa gtaagtaatt
     2341 tccattatct aattcaaaaa catctttagt ctttccttga tacacttttt tcataaacaa
     2401 cctccaaaaa gttaaattat tatattgaat aaggcattag tgtcaaaaaa gttctgatat
     2461 tcgcatgaaa atttatattg ataccttata tttaatatta tatcacaaat agaagggaaa
     2521 tttccgaaca attttataaa aataacaaaa aaagtacatt ttataaaact tataataaga
     2581 gaactaatat aaaatgttag tgtcgtttat attgacacac ataagtttta atataagaaa
     2641 cttttgacac actttgacac acatttaatg ataattttat aaaatgcatg aatacaatgc
     2701 ttacatatat tggcatgaaa gttgctttag atatttaaca tataaacaat agggatatat
     2761 tctcaaataa aaagtagggg tggttaatag tgaagtcagt tagattctat ggtataagag
     2821 acacaagagt ggaggatgtg gatgtaccaa aaatattaga aaaagatgat gtaataatta
     2881 aagtaaaagt agctggaata tgtggttctg atatatcaaa atattcaaag acaggtccac
     2941 atatggttgg agaaatttta gggcatgaat tttctggaga agtggcacag gttggtaaag
     3001 aagtaaggag ttttaaaata ggagatagag ttgctgtttg tccagctatg ccatgttttg
     3061 aatgtgatga atgtaaaaaa ggcttgtatt caagatgtaa taatgtagca ataataggaa
     3121 ataaagaact tggaggttgt tttgcagagt atacaaaggt gaaagagaga aatttaataa
     3181 aaataccaga tgagataagt tatgagactg ccgctgcgct agaaccagta tgtatagcag
     3241 gacatggtct ttttaggtca gaagctaaag ttggagatac tgttgtagta cttggtacag
     3301 gtccaatagg attattttca atacaatggg caaaaatatt tggttctaca aaaataatag
     3361 ctgtggatgt atttgatgag aagttagact tggcaaaaga gttaggggca gatatttgta
     3421 taaatgcaaa agaaaagaat attgtagaag aaataaaaag gttaactgat ggatatggtg
     3481 cagatattgt tattgaatcg gctggtactc cactgacatg tggtcaagta ctgttgctag
     3541 ctaaaaaggg aggtacagta ttgtatgcag gtgtaccata tggggatgta gctttgacta
     3601 gagagcaatt tgaaaagata gtaagaagtg aattaactgt aaagggaact tggtttggaa
     3661 actcctttcc attcccagga aaagaatgga gtgctggatt gtatcatatg caaaagggtg
     3721 atatgaatgt tgaaaaatta gtaacacata gaataaattt agaagaagta ccagcttatt
     3781 ttgaaaaagt atacaaaagg gatattttct ttggtaagat aatgattaat atagataatt
     3841 agattagagt actaaataat aatccatata aaaaaggtta agaaaggtat gattcatatg
     3901 gcaggaataa ttatagccac acatggaaat atggctaaag caatgttaga aagtgctgaa
     3961 ttaatagtag gcaaacagga aaatgtagaa actttaggat taaaccatgg tgacagcata
     4021 gatgagttta gtagaaagtt agaagatgga attgagaaat ataaggatga aggggtttta
     4081 gtattgcttg atttttatgg cggaactcca tttaatacat ctgctattat aataaataaa
     4141 tttgctaagg tatgtgagtt agaatgtttg acaggtgtaa atttacctat gcttttagag
     4201 ttatttttaa acagagatac tatgaagtta aaagacttga agaatttatg tgaggaagtt
     4261 gggatatcag gaatcaaaga tgttaaaact gttttaaagt tgtaaaataa attaaaaatc
     4321 tggaggaata attatgagta atatagcatt aacaagaatt gatgatagat taatacatgg
     4381 gcaagttatt actgcttggt gtaagattac atcggcaaaa aggatagtaa tcgtggatga
     4441 tttagtagta aaggacccat ttatagttca agttttacaa atggcagctc catcaactgt
     4501 taaggttgaa gtacatgatg tagaatcagg tgctgaagtc ttaaaatcac ataatggaga
     4561 tgaaaattta atagtattgg tcaaataccc taaaactgtc ttaggtttag taaatagtgg
     4621 ggtagattta aaggaattga atgttggagg tatgggagca gggcttggta gaaaaagttt
     4681 ttataaaaat atatctgtgt cagatgaaga aaaagaaata tttagagagc taatatcaaa
     4741 agatgttaaa tgctttatac aaatagtacc agatgcaaag aaaattgatg taggaacatt
     4801 attataaaga agactatctt gtaaatcttt aaaagaaaat agggaggaag aatatgcaca
     4861 tttcactaat tcaagcagta ttaatagcaa ttttttatta tctttcttgg agtccgtggt
     4921 tgacttatgt tggatttttt acatggaata gacctctatt ggctggattt gtaacgggaa
     4981 ttatattagg agaccctgtt caaggagcaa ttataggagc aggtataaat atgatatatc
     5041 taggttttat atcagcaggg ggagctcaga tgggggaccc agcttttgca ggatatgtag
     5101 gtactgcttt agctatagca tctaagctag atgtaagtac tgcaatggca atagctgtac
     5161 cattgggaac tgttgcaact gtactttgga ttggaaagat gacagtaaat tctttttttg
     5221 cacattgggc tgatagagag gttcaaaaag ggaatgttga tagagtagca tttataaata
     5281 tagtaccacc acagatactg ttatttgcaa tgagttttat accagcatta ttagttgttt
     5341 attttggtcc aaatgcgata gatggtatgt tggaagtaat gaatgataat gtgttacatg
     5401 tatttaatgt aattggggct atgttgccag cacttgggat tgctatgaat ttaaaattga
     5461 ttggaaacaa gtttactatg ccctttttca tattaggaat actaatggct gtttatttta
     5521 aagtagatat aatagtaata tctgttattg gagtaatact tgctcttaca attacttcaa
     5581 ttaagtacgg aaaagatagt gctacatctt aaatacatgt gagggggaaa atattatgtc
     5641 agatataaaa aatgaagtat ctaagaaaac gttaagcaag aaagatgtaa taaaatcatg
     5701 gttaagatgg tttttctttg cacaatctaa ttacaattat gaaagattgc aatcaacagc
     5761 tttttcacat tcaatgttgc cagtacttaa aaaattatat ccagataagg aagagttaaa
     5821 acaagaagtg gagactcatc tagcattttt taacacagaa cctatatgtg gatgtgttat
     5881 acatggaata acaatagcta tggaagaaga aaaagctaat ggagcagatg aaataagtgg
     5941 agatggtatg aatgctatca aaacaggctt gatgggtcca ctagcgggaa ttggcgatac
     6001 tttaactcaa ggagttataa ctcctatagt gttggctgtt tgtattggtt taactgaggg
     6061 aggagcatca attgcaggac ctatattatt tgtaatagct caatatataa taatgacttc
     6121 aatttctttt ggaatgtgga caaatggata taagtatggg aaaaaagctg ttgaaagtat
     6181 acttcaaggt ggaatagtaa ataaggttat agaaggggct tctatactag gtaccttagt
     6241 catgggggga cttgtaggta ggtttattaa tttatctaca ccaataagtt atactagtgg
     6301 tgattttaaa tttagtcttc aaacggattt acttgataag atatttcctg ggttaattcc
     6361 tttggtactt actttattag tactctttgc tttaaaaaaa ggtctttctc caataaaaat
     6421 aatgtgtata ctgatagtag tgggagctat tacaggtatt ttaggactat tttaaataaa
     6481 tatatattgg tgtaagttag gattctatat ctattgatta tatttgtagg atgttggaaa
     6541 atttatatta aaaagtttgg caattagggt aattttataa tagattaaat tttaaactta
     6601 tgtaagctat atgtagtatt taatatttta aaaagtatct cagaaagatt tttaatcaga
     6661 atgaggtgct ttttcatttt tatataaaat attaagtaat ttcaaaaaaa tataaattta
     6721 tttaatttaa ttaaaatatt ataacatatt agttacatat taaaatatat aatcgagata
     6781 ttgttaagac attgtcgaga tattataata tataactaat gtattataaa taatagttgt
     6841 aaacttcctg tgacatatat tttaaatgct tattcttaaa tttttgcatt atttattaaa
     6901 atttattatg ctttaacgtt aaattgagtt tgataattat aaaacaaata atcaaaaata
     6961 gtgtaaaaat taatactttt tgttaatatt aatagataaa gataatctaa ggtggtgatt
     7021 aaactgacaa tagaagaata caaaaacaaa tactttactg gaaataaaat cgtactttct
     7081 ataaatagtt ttgataataa agaactatac tatcatgaaa ctgaaaatat attaaattta
     7141 ataaatgaaa tagataaatt aacatcaagt aagatattta aaataataga agataaaaat
     7201 gatttggcac tagagtctta ttattctagt gtaatagaag gtgctttttc aaatagaaag
     7261 attgcaaaat ctataattag aggaaagatg aaaccaagta ataaaagtga atatatgatt
     7321 tacaataatc atagagcctt agaatatgga ttagacaact tagataaatt atatagtcac
     7381 aaatttatat atgatttgca tcatatattg ggcgaaaact gcttggattc agaagaatat
     7441 gagtatagaa ctgaaaaagt atatgtatgt gattcaaaag gtgagattat acatataggg
     7501 cttgaacctt tgaagatata tgactttatg agtaaattaa tagattttat ggaaaatagc
     7561 aaagtaagta atttaattaa aagtgctata attcattttt actttgtata tgttcatcct
     7621 tttagtgatg gtaatggaag aacatcaaga gccttatcat atctatattt gattgataaa
     7681 ggatatgata ctttcaagga gttttcaata tcatatatga tatctaaaaa cagaactaag
     7741 tattataagg ctatacttga tgtagaaaac aaaggaaata atttaactgt atttatagag
     7801 tttatgctca agtctataat acaatctatt aatgagatgc gtaatatgca tgatagaaaa
     7861 tctttagaaa gtatattaaa agaggaattg tttgaaaatg acataactct atcagcaaca
     7921 gaagaacata tattaaaata tatctgtagt aaagataact attctatgac attagaaaat
     7981 tatataaaaa agaataaaag tagatactta aaggctggaa taaaggaaat tgaattagtt
     8041 gaccagttga tggaagtgtt taataatctt gaagagatag agattttgtc taaagaaaaa
     8101 aatatatata aagttaatga taagtactta aaaatgcttg atatagacta gtttagataa
     8161 tctggagagg gaatatatga ataataagaa aaaggcattt ttgatttctg gggctataaa
     8221 cgtaatttca ttaattattg ttttttcatt gtttcattat aatattatag aaaatagtac
     8281 acaaaatatg ataataactg taatcttaat ttttattttt gaaatgataa aaataaaaat
     8341 catagagaaa tactataaac tataaacaag aaatatcttt tctaagagca tattgtacaa
     8401 tatgctctta atttagttat atagatttaa tataaataat gtattttttg tagatgattt
     8461 atgtaagatt tatgaccaat ttgtgtatga tttgtgttct ttaatcatga caatattata
     8521 ttgtaaaaaa taaatttaat aaaaaatgac ttggaattta tattctaggt cttttttata
     8581 taaaaattta ataagtttca aatgttggta actttgacag tagaaaacgt ctattgagat
     8641 aacgaggatt ctaatttagg gggagatata ttagcactta aagtagtata tgcaacagat
     8701 ataatcttta caaaagtaat ttgaattaaa atgtaaatat aaagaataat taaaaaaata
     8761 tcgtaattat ttgtttttca tatggataaa attggttatt taatatataa ttacaagtat
     8821 aaataataaa atttatttgt aaaaagagta gcacttttaa ttctaaaggc tacttttttt
     8881 atacaaaacc ctatttttta taacaattca ttaacataaa aatcaaactt accttaaaat
     8941 ataaatttac aaatttttca atcaataaga aaatttcttt attccttaca taatgactgc
     9001 aatgtaacaa aataattaca aattaattat gaaatcatta tagttagaaa cctctttaaa
     9061 attagatatt aagtatcctt tagaattaga acgggaacaa agttaaaaaa ttaagtaata
     9121 tcaaatggac aagggggaag aaaataaaat gaaaatacca aagaagattg ctgcaatgct
     9181 tacagttaca atgatagctg gaagttcaac agcaggcata gctagtgcac aaactgtggc
     9241 aacaaattta acagggcaag aaagatatga aactgctgtt aaaataagtc aagatggatg
     9301 gaaaaatgct gatgaggtag taatcgtaaa cgactcatct atagcagatg ctctatctgc
     9361 aacaccattt gcaaaagcaa aaaatgctcc aatactttta acaagtaaag ataaattaaa
     9421 tgataagacg aaagcagaaa tacaaagatt aaaagctaaa aaagtatact taataggtgg
     9481 aacttctgta ttaagtacaa atatagaaaa agaaataaaa gatttaaaaa tatcttttga
     9541 gagaatctct ggagctgaga gatatcaaac atccttagaa ttagcaaaga aattagatgc
     9601 tataagtgat gttaagaaaa tagctgtagt taatggtgaa aaaggacttg cagatgctgt
     9661 aagtgtagga gcaccagctg cacaaaacaa tatgccagta atacttgctg attctaaaaa
     9721 tggaacagca gttgcagaca aatttataaa agatgctgga ataactcaat cttatgtagt
     9781 aggtggagag agttcaattt ctgaagctgt taaaaataaa ctaccaaata gtacaagact
     9841 aggtggaaca gatagaaatg atacaaatgc aaaagtaata aaagaatttt ataaaaagac
     9901 agatttaaaa aatgcttatg taactaaaga tggtatgaat aaacaagacc aacttataga
     9961 tgcactagca gtaggtgtat taggtgctaa aaatcaatca ccagtagttt tagttggaaa
    10021 gaacttatca gcttcacaaa aatctctagt taactctaag agttttgata agataactaa
    10081 agttggtggg aatggtaatg agacagcatt taatgaaatg aagtcattac aagaagtaaa
    10141 aacagttgaa gctaaaacaa tttctgaatt aaaatcagct atagataaag ctactgctaa
    10201 tgatgttata aactttaagc caactagtga ggttaaagaa gcatttacaa tacaaactga
    10261 taaagcagta acagtaaact taaatggtac atatacaaag actgtaacta tcaacatgcc
    10321 taatggagat gtaaataact atgcaaaagt ggatgatgta gttatagatg atgtaaaaga
    10381 tggaactttt gttaactatg gaaaaataac taacttaaag gttaatgata aaaatggagc
    10441 caagatagaa aataattcta aaggtgagat tggtagttta acagtagcat ctggagcaag
    10501 tcaagtaaaa gtgactaatg gtggtaagat aactacagtt acaaataatt ctaaagggac
    10561 tactatagat aataaaggta caataagtag tgttaaggga gataattctc caacaataag
    10621 tggaaatagt ccaagttcta actcttcagg aggaagttca tcaagtggag gttcttcaca
    10681 tggaggaggc tcatcttcag gaggaagttc ttctaatcaa acatcagtaa ataatgaagc
    10741 agcaaaaata acaagcgtgc ctacaccagc aaaagatgca actagattaa ctatgccatc
    10801 agtatcaagt ggatatacta tagcaataaa aacatcaagt aatgagagtg ttataaaaaa
    10861 agatggaaca ataatacctc caaatacagc aacaacagta aaattagtat ttacggtaac
    10921 acatacatca agtggtaaaa cagcagatac aaaagaatta tctgtaacag taccagctaa
    10981 atcaacagat gaagaattac aagcagcatt agacaatgaa gtggcaaaaa taacaagcgt
    11041 tcctgcacca gcaaaagatg caactaaatt aactatgcca tcagtatcaa gtggatatac
    11101 tatagcaata aaaacatcaa gcaataaggg tgttataaag gaagatggaa caataatacc
    11161 tccaaataca gaggaaacag taacattggt atttacagta acacaagaat caagtggtaa
    11221 aacagcagat acaggagaaa tagatgtagt agtaccagcc aaatcaaca
//
LOCUS       DAEORZ010000136        11177 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEORZ010000136 DAEORZ010000000
VERSION     DAEORZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11177
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.137"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..623)
                     /locus_tag="KRH39_003066"
     CDS             complement(<1..623)
                     /locus_tag="KRH39_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG8512951.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNID"
     gene            complement(695..1048)
                     /locus_tag="KRH39_003067"
     CDS             complement(695..1048)
                     /locus_tag="KRH39_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG8512952.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVDSFAKPQFVYSWK
                     LVSTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLHAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1126..1329)
                     /locus_tag="KRH39_003068"
     CDS             complement(1126..1329)
                     /locus_tag="KRH39_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG8512953.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            complement(1719..1949)
                     /locus_tag="KRH39_003069"
     CDS             complement(1719..1949)
                     /locus_tag="KRH39_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8512954.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1951..2358)
                     /locus_tag="KRH39_003070"
     CDS             complement(1951..2358)
                     /locus_tag="KRH39_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8512955.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            complement(2717..3166)
                     /locus_tag="KRH39_003071"
     CDS             complement(2717..3166)
                     /locus_tag="KRH39_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8512956.1"
                     /translation="MELIEWNYAYITELVEFANDKEIANNLRDNFPHPYREQNARDFI
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            complement(3180..4106)
                     /locus_tag="KRH39_003072"
     CDS             complement(3180..4106)
                     /locus_tag="KRH39_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512957.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKISVKQGNETVEIADG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4231..5013)
                     /locus_tag="KRH39_003073"
     CDS             complement(4231..5013)
                     /locus_tag="KRH39_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBG8512958.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKKAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5169..5789)
                     /locus_tag="KRH39_003074"
     CDS             complement(5169..5789)
                     /locus_tag="KRH39_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG8512959.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5877..6575)
                     /locus_tag="KRH39_003075"
     CDS             complement(5877..6575)
                     /locus_tag="KRH39_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8512960.1"
                     /translation="MYKLSYVVRVSTKDLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALVSQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSDDESKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            6676..7491
                     /locus_tag="KRH39_003076"
     CDS             6676..7491
                     /locus_tag="KRH39_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG8512961.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLFFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHKDKLPKIT
                     PHTLRHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSARAEMERL"
     gene            8163..8930
                     /locus_tag="KRH39_003077"
     CDS             8163..8930
                     /locus_tag="KRH39_003077"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG8512962.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNEKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9258..9935)
                     /locus_tag="KRH39_003078"
     CDS             complement(9258..9935)
                     /locus_tag="KRH39_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512963.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10085..10402)
                     /locus_tag="KRH39_003079"
     CDS             complement(10085..10402)
                     /locus_tag="KRH39_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8512964.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10424..10762)
                     /locus_tag="KRH39_003080"
     CDS             complement(10424..10762)
                     /locus_tag="KRH39_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8512965.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3715 a         1755 c         1584 g         4123 t
ORIGIN      
        1 ttatcaatat ttcctgattg aatatgctta atgtttgatt gaattttttt atatggccaa
       61 tcccaccatt taatttctaa taattttgaa attgtatcat tattaaatct cttttttata
      121 atttttgcag gtactcctcc aacaatagaa tacggtggga cgtcattagt aactactgct
      181 cttgttccaa taattgcacc atcaccaata tttacacctg acataattat agaatcataa
      241 cctatccaca catcatttcc aattataatg ttacccttat tgtcccaagc gtccgttata
      301 tggttgatat cgagtttcca ttcttcataa aagatgggaa aagtatatgt ggattttgat
      361 ttcattgagt gatttccact agtcattaga aattttgcat tacatgctat tgagcaaaac
      421 ttaccaatta ttaatttatc attgtttata gggtaatgat acaatacatt gtttttctca
      481 aaatctcttg ggtcattata aaagtcatta tatattgtgt aatctccaac ttctatattt
      541 tcccttgtta ttacattttt aagatataca gtttgataat cattacttct tggatatacc
      601 ttatttgaac ttggaatagt catatttaaa tctctccttt aatatatatt atgtttttga
      661 ctattccact actacaatac atctcttcac agaatcactc cttgttcaga atcttcataa
      721 gataatggcg tcctttttta gtgttaagtc ggatattctt tcttagtcca ttctttttag
      781 cgtgtaactg acaaactgtc attttcttac caatagtgtc aatgctatca tcaaggtcat
      841 tttgtattgc tttttcctta tcccttaatg atatatcatc acgttttccc tttgggggtt
      901 tatcagtaga tacaagtttc caagaataaa caaattgtgg tttagcaaat gaatctacat
      961 acttatatgc gtatcttcca tcttttctct ggctctctcc agttcttaaa attctatctt
     1021 tattatctcg tcttttttca gttgacatga taagaagctc ctctcaataa ccgaattgag
     1081 cttgatacgt catatgaata ttataacata taaggctcga ttttattaga taacgtcaag
     1141 tgtatcaacg attttctcaa attgttttct ctttatttga atgcgattgc tattcataat
     1201 gacccagcca gaatcaaggt tttcatcagc aagtttacgt aattttttct ctccaatacg
     1261 aaagtatttt gacgcttctt caatcgtcaa ggtatatttt tcccaaatag gtacatcagt
     1321 attgttcata gaatgtatca ctcctttcta tgttcgtagt tcagaagtag atagatggct
     1381 ttggtaagct ccattggtat ttcaaccaat cccattctta tgggcagaat cgtgtcatac
     1441 aggtgtatca ttatactgtg gtacaggtta cggcaaaaca accgttccaa aggttattca
     1501 caaatcacta tatagtagct ctcttgatta aggtcatggc gtatagtatt tgttggctat
     1561 ctgtatcaca aacgtatcta tctactttat tcagttgtca aagaacagtc ggcattgatt
     1621 tgcctataaa aaatcacaat agcttgtggg cttttataga caaataatta gtgattctcc
     1681 atgtaccaaa tagcttgata catggagaga gggcgagatt aagacaaatt aaatctcaaa
     1741 atagcagtga ttagttttgt tcctagcatt cgctttgttt cttcttctac atgaatatag
     1801 ggatttccgt attcatcaaa taaagttttc tgtgaaagac gaatgatata gtgttcaaaa
     1861 tgtttaagta ttccgtttat tgcatttaca tcgccatgta cagcaagtaa aataattgta
     1921 aaaggtaata cttctttagt aggtttcatt tcaatgttcc tccatgagtt ctttcaactc
     1981 ttttagtgca tttgttttgt gataatgtat ggtattttct gctaaatcca tcaatgttgc
     2041 tatgtcagca tttgtcatgt tgagaaagaa tgaaagtaga attatatctt gttttctttt
     2101 tgtaagtttt ccgattgctt gtgccattaa tacatcttca attcgtatgt tgtagtcgta
     2161 agaaaccata cagatgtagt tagaatcata atcatctaaa atactcaatt tacttaattc
     2221 tgcaagtgac aaagattcga gagataccaa ataatcattt ctgcgtttat tttcgtcata
     2281 aatatctcta gcatagttac gcaaaactgt tttacagaaa ctgtcgaatt gatgttcaat
     2341 tgtcttttca tcaaccataa gatttcaccc tctttccatc gcaaggagtt aaataacctt
     2401 gcttactcct atcacggtgg gagggcgtta aatcttcgat aaatttcaaa aaaagagaaa
     2461 aaaatacaca ctctgcctat agggcaaaaa tgtgtattct ttaaaacgta tttaatttat
     2521 attggttgta agccaccacc atattgatgt atggaaatat gcattggcta gcttttcata
     2581 aaaatacaaa gatgcataag aaattaatag aggggaaaaa cttattctgt tattaaaaag
     2641 taggttaatt ctaataaatg ctattgcggt cgaattaaac tatatacatg gcaatcaatc
     2701 atgtttctat tctttattat gaattgtgac agtattcttc aatcttaaaa ccacattttt
     2761 ccaatacttt acttgaccct ctattgtagg aatatacatt agcatgaatt ttgttaaggt
     2821 tgtatttttc aaaggcaatt ttgcatattt cttttactgc atttgttgct atccctttgc
     2881 cccaatagtt cctagcaagc cagtaaccta attcagcatt tttattaata gaggtatctt
     2941 cttcaaatgt taggctgata cttccaatag ccctttcttg ataaaaaata gcatagctaa
     3001 gttgtttaac atctggagtt gccaaacaaa aactaataaa atctctagcg ttttgctccc
     3061 tataagggtg ggggaagttg tcacgaagat tgtttgcaat ttctttatca ttcgcaaact
     3121 caactaactc ggtaatataa gcataattcc actctataag ttccattttt cttctccttt
     3181 tatgaaacag ctacaagaaa tctttctaag ccaataccac ttatcaaaca gcgttctttt
     3241 ttatttccaa gcatttgata agtccagtca acaaatccac cgtcagctat ttcaacggtt
     3301 tcatttcctt gtttgaccga tattttgaaa ttgatacctt tataataatt attatcaaca
     3361 tcatcattat gaattgataa ctctatatta ggaaaaataa gtttgatagt ttcaaccatt
     3421 ctgtcgaaaa atccgtcatt atctttgtat ccgtttcttt tgcgtagaat aatagagaaa
     3481 cgaacatttt ctatttcact taatatatct ttataatagt ttaagtgctt ttctaacaac
     3541 attttttcac aagtgtaaga ccctgtatct ataccagatg aaaccataca atataagcca
     3601 aaatgtgaat ataaaccttt acctacaaaa ttttgtgctc ttattaccct agcagtagta
     3661 gccatgtgga taggtatggt attattttct tcttttcttt ttaatttatc agcaataatt
     3721 attgccaaca tatttgaggg gtctgcaagt gtttctgtcc ctcgtagagc acttactaca
     3781 ttatattgat ctacggagcc aaaaacgcta cagctaccta atggagcaga gggagaaagc
     3841 ataatcgtct tgatatccat attgaatgct tttctcaata attttgactc caaagcatga
     3901 atttcttctg gatctatacc gctagggata gtaaatctat ttagttggta tgactttaga
     3961 atatcagttg cagttaaagt atttgcttgt ctatcaaaca cttctaatag aagtgagttc
     4021 aaatctgatt ttgatagagc tgataactta tcaactatat tcttatcccc aattttattt
     4081 gtaattctac ttaaaatatc actcatagtt atcctccata ctgttacttt caaaataatt
     4141 atagaatggt tacaacaact aatctaggtc atttgaccaa aaatgaaaaa acactgaata
     4201 tttactattc aatatttttt tactttgggt ttatttgatt aaatcttgaa aatactgtaa
     4261 tgcaaccctt tcagcttcaa cctttcggac aataggactt tgtgataatt cctctcttaa
     4321 ctcaagatac aggttgatag tagcctgcaa tgcattaaca cagcttttat attcatggtt
     4381 agcaaccgtc ttttctaatg cttcaatata ttggggtgcg tccaattcaa tttttctaac
     4441 tccacgaggt aatttaccta agagaagctg tattaatggg gcaagtactg attgtcttaa
     4501 aaaagataaa aaatcaattg tttcaaatag ttctctacgt ccaatcttag tgcaagcgta
     4561 atgaacccaa atccaaaatc tatcttcaat ccactgtaag tctggagttg ggaagtatgc
     4621 ttttttttta gaataaattt ctgttatgca gttgttttct tcatagagaa tagctgaatc
     4681 ttcaacccta tctagtaaac catctaaggt tgtaaactta aaatcaacat gaagaacagg
     4741 ggaatcatag agggaaatta tcaatcgagg ttctccaacg tgttcgccag taaaagcaga
     4801 aactaatgtt ccaaactgtt ccacaataag taatctctct tcgataactt cttcatagga
     4861 tttatcatct gctacaatca gaaaatccaa atcagaatat tcatctaacg attttgtgat
     4921 ataagaaccg ctaattgcaa gtcctattat cctactatct ctttttgaaa attctaatac
     4981 gttttgtatc attttctgtt gtgttttagt cataatataa cacctctcac tgtcttttat
     5041 tagctatcaa tccaacataa ttatgcatag gagtattata taatgaagaa ataaaaaaaa
     5101 ataggtcact tgacccgaaa taaaaaagca ttgaataatt attattcaat acttttttga
     5161 aattatactt atgttgtagg aagattcttt aatacctctc gctgattaat tacactaaat
     5221 cttttgtttt cataagcaat tagatgttct tgtacacatt catttatagc acgattaatg
     5281 cttctaattg gagcgtttac ttcgctagca agctgttttt tggtaagcag atttaattca
     5341 cttctataat agtgtaagga tatacacctc agaagtcgtt ctttaacact taaagatgat
     5401 aattcttcaa ttaattcagc atttacaact aatttactag atatttctcg aatcagaaat
     5461 tttgtagctt caaaatctcc ccttaaccag tttaaaaaat catctctatg aagtttatat
     5521 acattacaat ctgtgaaagc gacaatttca acgggttttt tcccatcatt aaattgttcc
     5581 atttctccaa aaaaactacc acttctataa agatgaatag ttgagatgtt tcctttcaca
     5641 tttaagatga atgcttctgc aataccgtca atcataagaa ttacatattc attttcttgc
     5701 tcagcaaaaa gaatagtctc tttcttttta tacgtttttt taacaagctt ctttttaata
     5761 tcttcattca taaaatcaat taaatgcata ttcatccctc cttaccaaat ctttgattaa
     5821 attatatcat ctttcaatta gttaggtaag atagtaaaaa tatatttaca tagaaactat
     5881 tccattatta ttttccagtt gtcatttttc tgcaaaaact tatcactgtc acgactggag
     5941 ataccagtaa ggaatgttag gatatcaatc gctaaacttt cagaatcctt tttacggctc
     6001 aaaatgacgt aagggtcaag caatcctttg ctttcatcat cactggttaa attgacaatg
     6061 tttatctcat gggctatttc tggtagcgtt tctttccatt tgccacgttc tgctttaggg
     6121 tcaactatga ccgcctgtcc gccgaatagt accgcatagt aaacaagcat attgttactg
     6181 aataatttac caccacccaa tgaaccgata aacgcagacg ctaacgcatt ggtaatagaa
     6241 ccctttaccc cttgactaac aagggcaggt tttagataga cattctttcc agtgtccaga
     6301 ttgtagccaa gatagatatc ctcatgttcg ccaagggttt gtgtcgctcc aaaaccaagt
     6361 ccagccaaaa aatccgaagt gacatattga atataatcat tgatgtaacg tttgctgaca
     6421 ggaataaatt cgccatgcaa tcccaacata tccccaaagg gacgcacgag cttgattgat
     6481 aagtcgtcgt aaaagtcttt gacttcatca caacgccttt ttagctcgtc aagatccttt
     6541 gtagatacac ggacaacata gctaagttta tacatagatt ctttacagtt aaaagtctgt
     6601 ttttaaaaaa atgtataaat tataaatcaa aatatagata ctatcaatca cccactaggt
     6661 gcaaaaagca ttgatatggt taaattatca gacgctaaaa gctgggctat ccgaatgagt
     6721 gaagatgggt gtgcttataa atccattgac aattataagc gctcactgaa agcgtcgttt
     6781 ttcatggcaa ttcaagacga ctgtattaga aagaatccat ttaattttca gttaagtgat
     6841 gttttggaag atacaacaga accaaaaatc gctctaacac cagaacagga aatgggctta
     6901 ttattcttta tggagagcga taagacttat tgtaaatatt atgatgatgt tgttatcctc
     6961 ttagaaacag gactacgcat ttctgaatta tgcggtttga ctttagaatt ggatatgaaa
     7021 aataaagcca ttatggtaga ccatcaattg ttgagtgata ctaagattgg ctattatgta
     7081 gaagttccaa agaccacaca gggtaagcga gaaattccaa tgactgaaag agcttatcaa
     7141 tcatttgaac gtatcgttga acggcgtaag aacgctaaag cagaaccaat cattgttgat
     7201 ggttaccaga acttcctatt tcttaaacaa gatggattac caaaacaagc aactcattat
     7261 caaagcatgt taagagcgtt agtcaagaag tataacaaga cccataagga caagctacca
     7321 aagattacgc cacataccct taggcataca ctctgtacaa atatggctaa caaaggaatg
     7381 agtcctaata tattgcaata tattatgggg cataaaaaca ttactatgac gcttggctat
     7441 tatgcacatg gttcatttag ttctgcgaga gctgaaatgg aacggttata agcctagtag
     7501 tatgactaga gtttactact tatttactac ttttgatggc attcttatac ggttaaatgc
     7561 caacttatgc aaagtatctt ccaaaaagaa aatgccgata ctccttgtag atacaggata
     7621 tatcgacatt taccaactta tgaaaagata atcaaattta atataagttt ttcaaataaa
     7681 aagataccaa attctttttc gagaatctat ataacattac ttaagcttgg ttggggtata
     7741 aacaattgag tagccacttt tgtatggtgt tcaactttag tcaaaactct taagtaatgc
     7801 aaatgattta gattcattaa tattcaatct cttcataagt agtgaaattc gagttatttc
     7861 tgcataatta tactatgatt tatagattaa ttctattagc atccagaata atattgttta
     7921 tatgagtaaa atttaaagaa tattgtgaaa attataggat gttaaaataa atttaggtaa
     7981 aaaagaagtg tttgtatatt atagagtacc ttttctaaaa tatgtgggat tgttttaaga
     8041 attagaataa aaattatttt taaattagta tttttataat gtatagtatt tttaatgtat
     8101 aattaaatta tgcagaaaat agaatttaaa gtcctataga atttctgatt atggaggaga
     8161 atatgaaaag aaaagttcaa gtaaaaaata ttactattgg agaaggtaga cctaaaattt
     8221 gtgtaccaat tattgggaaa aatgaaaaag atattataaa agaggctaaa gagttaaaag
     8281 atgcatgttt ggatattata gagtggcgag ttgatttttt tgagaatgta gaaaatatta
     8341 aagaagttaa ggaagttctc tatgaattaa gaagttatat acatgatatc cctttattat
     8401 tcacatttag aagtgtagta gaagggggag aaaaattaat atcaagagat tattatacta
     8461 ctttgaataa agaaatttct aatacaggtt tagtagattt aatagatgta gagttattta
     8521 tgggagatga ggtcatagat gaagtagtta attttgctca taaaaaagaa gtgaaagtaa
     8581 ttatatctaa tcatgatttt aataaaactc caaaaaaaga agagattgta tctcgtttat
     8641 gtagaatgca ggaattgggc gcagatttac caaaaatagc tgttatgccg caaaatgaaa
     8701 aagatgtgtt agtgctactt gaagctacaa atgaaatgtt taaaatttat gcagatagac
     8761 caataataac aatgtcaatg tcaggaatgg gtgttataag tagattgtgt ggtgaaatat
     8821 ttggttctgc attgactttt ggagctgcaa aaagtgtttc agcaccagga caaatatcat
     8881 ttaaagagtt aaattcagta cttaatctat tacataagag tattaattaa attaattata
     8941 tagaaatatt agacggtatt tttgaagata taaattaaat ataaaaaact tttcagatag
     9001 taaaatttaa attgggcaat tatatctgaa aagcaactat ttagtgtgta ttttgaaagt
     9061 gatatagaaa atcttataaa tcaactatgg catatttaaa atatgccatt aaaacctact
     9121 agacattaga ttatagatgg tatactagca tttctattat ttatagatag taagttttca
     9181 taataactgt tttatctaaa gtagaagata tatttgtatt atgtatattt gtcattattt
     9241 ttttgtaatt atgtatatta atggttatat atgtaatata atattatcac tctatttttt
     9301 gtatattgta cattacaaca tttatatcta tcgtcatcaa ttacagtaga atgtatggga
     9361 tacttatttt cacttaacca atatttttgc atattctttg tccaagactt tggaaacttc
     9421 aatacttttt tgtttattat aacatcctca tcttcaaaaa tattaacttc tctttctatt
     9481 tttatttttc tgttgtatat cttattgata gattctttgt cgagttttat gtccctatac
     9541 atatcagggc aatagcaaca aacataattt cttttttttc gacgtttaaa tttcacaact
     9601 ttctctatgc taatttctaa tttttttgta ccatctttta taaatttaat atcttctatg
     9661 ttctcgcttg aaaaaatttc tgtcaatctt aaagtatcta cagtttctct aattatttgc
     9721 tttttataag aagagaatcc atatccaaaa gaaaggttta atttattagg gatgtagcca
     9781 tttttatatt taaacatctt aattttttcc aattcagcat tatctttaaa tttcaacttt
     9841 tcatacctaa aagttacttt gctaaaaaga gtatattcta catcaatata ttcttttgca
     9901 atcttaaatt tatcgctacc ttcaatcaca atcatagcct atctccaatc gttttatatt
     9961 aaaataaggc catagaacat ggtctaatag tactcattag cactataaga caatgttaac
    10021 acaaaaagac aaaaaaaggt tatttatttc aaataacctt tttttttata taaaatataa
    10081 agaattatat tttattcttt tttatgatgt ctataatctg tgatatcaga tttagttctc
    10141 ttgaatttaa gtcttttaac tcttcattta tatcattttc taaatatgtt tttccagtgg
    10201 aagtaatatc ttttataaga acatctgcag aaatatttaa tatatttaca aattttatga
    10261 atgttttaag acttggattc tttttaccac tttcgatttg agttatatac caaggcgata
    10321 ctccaaccat atttgcaaat tcttttgaag ttaaatttgc ttttttccgt tcttctttaa
    10381 gtcttttacc tatataattc attattgtta ctatttccct cacttatatt tctttgtatt
    10441 taatgatgca ctttattaac tctagcattg caatttgttc tttttcagtt aatttattaa
    10501 tcatttcata acattctaaa ggtacagagt tatcaacgcc ttctgttgta ataatatttg
    10561 caaataacaa ttcaagtggt atgtctaaag cagtagcaat tttcattata atattgatag
    10621 atgtatttgt ctcagctctt tcaatctgac caatatatgt tggatgtaaa cctgcctttt
    10681 ctgctagaaa ctcttgacta tatcccaatt ttgttctata gacttttatt ctatctccta
    10741 ttatttttcc tatatttgcc aattattatc cctccacttt agattatatt catagtttaa
    10801 gttgttttat atagactatt tatacttttt tagtctaaat atagactata tatgttttta
    10861 tactgaaata agtgctattt atttttgtat agtctgtatc tacaataaac aaatatggat
    10921 aaatcatatt tttaattaga gaaatatttt cataatttca tagccatact acaataataa
    10981 aacgaattaa taatatgtta attatagcat tttttattgt aattacatat tagatatgta
    11041 gaatatattt tgaaaattaa aaaaattttc tattttatat attgaacaat ttatgtaaat
    11101 agaaaatttt tcaataagac ttttgagttt aatcattata ttggtaaggt aaacattttt
    11161 aaatctaatt atatata
//
LOCUS       DAEORZ010000137        10971 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEORZ010000137 DAEORZ010000000
VERSION     DAEORZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.139"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            19..1719
                     /locus_tag="KRH39_003081"
     CDS             19..1719
                     /locus_tag="KRH39_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512966.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            1889..2506
                     /locus_tag="KRH39_003082"
     CDS             1889..2506
                     /locus_tag="KRH39_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512967.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            2867..4060
                     /locus_tag="KRH39_003083"
     CDS             2867..4060
                     /locus_tag="KRH39_003083"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG8512968.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            4078..5370
                     /gene="lysA"
                     /locus_tag="KRH39_003084"
     CDS             4078..5370
                     /gene="lysA"
                     /locus_tag="KRH39_003084"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBG8512969.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            5587..6246
                     /locus_tag="KRH39_003085"
     CDS             5587..6246
                     /locus_tag="KRH39_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG8512970.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6401..7486)
                     /locus_tag="KRH39_003086"
     CDS             complement(6401..7486)
                     /locus_tag="KRH39_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG8512971.1"
                     /translation="MIIISRENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            7677..8393
                     /locus_tag="KRH39_003087"
     CDS             7677..8393
                     /locus_tag="KRH39_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG8512972.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            8451..9029
                     /locus_tag="KRH39_003088"
     CDS             8451..9029
                     /locus_tag="KRH39_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG8512973.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            9423..10295
                     /locus_tag="KRH39_003089"
     CDS             9423..10295
                     /locus_tag="KRH39_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8512974.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT         4531 a         1149 c         1880 g         3411 t
ORIGIN      
        1 tttagagagg agtattaaat gaaacgatat cttaaaacat atggagttgc attaataatt
       61 gtaataattt atgcatttat taaattacct gtactaagat tagattttac atctttattt
      121 tcagtaggaa taattttctt tgttgtagca ggaatactag atatgatgtt agatagaaat
      181 gaaagagcat ctaaaatggc taaatataat ttttcaatag caatagcttt gcttgtgtat
      241 attgtagtag ttccctttat aacttctaca cctgtacttc atgcaaaatc atatagagaa
      301 ctattgggaa aagtaactga aagtaaattt actgatgaca ttagccctgt cagtgtaaat
      361 gacattcgtc ttgtagatga agatatggca atgaaattag gtgacaaaaa gcttggagaa
      421 gttcctgcaa tagggagtgt atccaaatta ggaaaatttc atatacaaaa tgtagatgga
      481 gagttgtact gggtagcgcc tcttgttcac agagacataa ttaaatggat aacatctcta
      541 agtggtacaa gtggttatgt aaaagtatca gctagtaatc ctcaagacgt ccaattagtt
      601 caagaaatag atggaaagcc aataaagata gtttaccaac cagaagccta cttacatcag
      661 gatttacaaa gacatttata catacatggt attgtaaatg ttggtatgac agatttcact
      721 cttgaaataa atgatgaagg aagaccttat tgggttgtaa gtttatatga acataaaatt
      781 ggctatggag gagcaaatgc aacaggaata gccactgttg atgcagaaac tggaaaaata
      841 aatgtttatg atgttaaaaa cacgcctaaa tgggtggata gaatacaacc acaaagcttt
      901 gtaacagacc aaattaaaga ctggggtgtt tatgttaatg gatttttaaa ctctgttata
      961 tcagaaaagg gagttttagt accaacagaa ggtacatcat tagtttatgg caatgataac
     1021 aggtcttatt ggtatacagg tataacatct tcaggtggag atgaatctac aattggattt
     1081 atgttagttg attctagaac aaaagaagct agattatata aacaaccagg agctactgaa
     1141 acggctgcaa tgaaatcagc tgaaggtaat gttcaagaaa aaaattatga agcgacgttc
     1201 cctgtaatgt ataatatatt gggtcagcca acatatgtat catcattaaa agataaggcg
     1261 ggtcttgtta agagagttgc atttgtatct gttgaagatt ataatgtact tggaataggt
     1321 gaagataaga atgaagcatt aagaaactat aaggatgcat tagaatctaa gggtaatgat
     1381 ttaaaacttg ataatgattt aaaagatgag gtattagaag gcacagtgac aagaataagc
     1441 ccagatgtgc gaggtggtaa cactaattat tatgtaactt tagattcaaa taaagatata
     1501 atatttaggg ctacttcaaa agtatctagc gaattgccat tgactcaggt tggtgataaa
     1561 gtcaaaatat cgtattcaaa agaagaatct ggtgtaatag aaatgtcaga atttgataac
     1621 ctaaatattg caaactttga agataaaaaa gcaaaagaaa aaacaggaac tgatgaaaat
     1681 acagattcca aagattctga aaatcaaggt aatgtataat ttaaataaaa agattgataa
     1741 tagtatttaa catagtgata gctaattttt atagaattta attctataaa aattagctat
     1801 tttattttaa ttctttgtaa atttaatgtc ataaaattaa tataatatta gtacaacttt
     1861 gcaaataata cttcaaggag aatgtactat gacgaatatg gaaaaagaga tattactttt
     1921 aattagcagg gttagatgtg ttacagagtc tcaatttaga aaaatttatg gagaccaaaa
     1981 aagatacaat aaaaaaaatt ttaaaaaaac tttgagaaaa atgtgtagtg aatatacact
     2041 aaaaaaatat ccttgtgatg tggattatta taattataaa gacctctctt atgtatacta
     2101 tttaaacggt agtaaacttt ttaagggaga tgatttaata aaggctctag taggatcaga
     2161 attagttgtt agaatcaata ctgcaggttg tgatgtaaaa agattttacc gaaatgttag
     2221 tgtagatgat gtgaaatacg atttatttat tgaatataca gaccaatttg gtaatataaa
     2281 gcaagtttta gttgatgttg acttaaataa tgaaatagac atttctaaat acgaaaatct
     2341 ttctaaaaaa atagataaat ctacaattcc attttttaaa gtaccaaacg ttttggtagt
     2401 tacaccagat aatctatccg aattaaatga tttagagaga tatgataatg agtatagcat
     2461 aaattttata gatttaggat taaataaact atttaattgt atataagcta actttagaaa
     2521 agaaaactat cgatttgcca cattttgtaa aataattgtt gcaatattat atagaatatg
     2581 ataaaactat attaataaat taattaaaaa taatgagcct agggtagagg tagctgtatt
     2641 caatagtatt caataggagc tggtaagcgt tgataattga tgaaagggta tatagccgaa
     2701 gaaattttgt tgacggacaa attttctgga cttatgcaga atatgcataa ggctgtcact
     2761 tttaaagtgg tgagctacaa ggttacttgg tctatgaaat ttacacgtcc aaagtatacc
     2821 tttgggcgtt tttatttatc aaaatttagc ttgaggggga gaaagcatga gtataattgt
     2881 tcaaaaatat ggtggaagtt cagtagcaga tactgaaaaa ataaaatcaa tagcagaaaa
     2941 tataatagaa cgaagaaaag aaaatccaca aatggtaata gttgtgtctg ccatgggaaa
     3001 atccactgat gaatatataa ctttagcaaa ggaattgagt aatgaaccta gtaagaggga
     3061 attagatgct ttaatgtcta ctggtgaaat gatttcagca tctttattat caatagcatt
     3121 aaatgcactt ggatgtaaag ctataagtta taatgcttat cagttaaata taaaaacgag
     3181 tggtcttcat ggaaagtctc aaattgatga tataaacgta aacagaatta aaaatagttt
     3241 agatgaagga aatgtcgtga tagtaactgg atttcaggga atcaacgaag atggtgatgt
     3301 aaccactctt ggaagaggtg gttcagatac atctgctgta gctcttgcgg ttaaattaaa
     3361 tggaaaatgt gagatatata cagatgttga tggaatttat tttacagacc caagaaaata
     3421 ttcaaaggct agtaaacttg atgagattga atatgaagaa atgcttgagt tagcgagttt
     3481 aggagcacaa gtaatgcatt caagaagtat agagcttgca caaaaatatg gtgttgaaat
     3541 ctatgtcgga cgtacatgtg gtacagaaaa aggaacatat ataagagggg gaaaagacat
     3601 gaaattagag gataaagtaa taacaggatt agctactagt gatgatgaca gttctataac
     3661 aataaaagat tttaaagctg aaaatatatc tagcttattt gaagatatag caactatagg
     3721 tataagtgta gatatgataa gccaaactgc acctatttta gataagataa gtgtatcatt
     3781 tacagttcca aaagaagagt tgggagaatg taaaaagata gtatctaaat atacagatga
     3841 agaacatgta gtgattgata ataatatcac taaattttct ttagtaggtc ttggtatgaa
     3901 aaatacttct ggagttgctg ctaaagtatt taagatattc aatgaaaatg gtataatgat
     3961 aaaacttata actacatcag agattcgtat aacttgtgct ataaattctg atgataagca
     4021 agtagctata gaaaaaatag cagaagtatt taatatataa aatttgggga ggctattatg
     4081 aaacttcatg gaactatgac tgtaaataat gatgaattat atattggagg agtgagttgt
     4141 ttagaactta gtaaaaaata tcaaactcca ctttatgtat ttgatgaagc tttagttaga
     4201 caaaattgta gagaatatat agaatatttt aaggctagag agggaaaaaa taaggttgct
     4261 tatgcaggga aagctttttt acctctttac atgtgtaatc ttataaatga agaacaaatg
     4321 tgtttagatg tagtttcagg aggcgagtta tatacagctt ataaatcaaa ttttcctatg
     4381 gaaaaagttt tctttcatgg aaacaataag actaaagaag aaataaacat gggattagat
     4441 ttaggtgtag gtaaatttgt agtggataat ttttatgaat tagatttgct tgaagcatta
     4501 tgtgaagata aaggtaaaac tcaagagata tactttagaa taactcctgg tatagaagct
     4561 catacacatg attatataaa aactggacag atagattcta aatttggatt tgctttagta
     4621 aatggagatt tatttagagc aatagaaaaa ttaagtgaat ataaaaatat taaacttgta
     4681 ggtcttcacg ctcatatagg ttcacaaata tttgatgtag aaccatacat agatacagtg
     4741 gatataatga tgaacttggt aaaagaaata aaagaaaaat tcaacataca actaacagaa
     4801 attgacctag gtggaggagt tggcgtttat tatagcgaag gagataaacc taaaactata
     4861 aaagagtttt gtgaaaccat aatcactaaa gcagaaactt catgtaaaga gcttggaata
     4921 gaagtaccta cattggtaat agaaccagga agatcaatag ttgcaaatgc aggaagtaca
     4981 ttgtatacag taggttctat aaaagatata aaagatgtta gggtttatgt aagtgtagat
     5041 ggaggtatga cagataatgt aagaccttcg ttgtaccaag ctagttatga gtgtagtatt
     5101 gtgaataaaa ttaatcatga ggctatgaat catgtaacaa tagctggaaa atgttgtgag
     5161 agtggagata tattaattgg agatataaac ataatggata ttaaaagtgg agatatactt
     5221 ataacaacat caactggtgc atatggatat tcaatgtctt caaattataa taaaatacca
     5281 agagcagcag tggtatcagt atttgatgga aatgataaga taatttgtaa gagacaaagc
     5341 tatgaggact tattagcttt agagatataa aatagagaag tatataataa aaattaaata
     5401 ttaaagttta ttatgagcag atttgctagg atatgtatct ctaaattaat ttttggttta
     5461 ttgtatcgtt cgtagcatat ctgcttattt tttgttttac taacaaattt cttgtaataa
     5521 atataaaata ttttaaaaac tttataatta tgataaaatt taaagtatat gacaaggagg
     5581 gatttaatga gaggaagagt aattttatta atatcgatat tatctatatt tcttgtagga
     5641 tgttcattta ataaaaaaga tgaaataaat ttagtagagc aaggaaaaac atatttagaa
     5701 aaacataact ataaaaaagc tatggaatca ttatcctctg cactggaaga agatagtact
     5761 aatgagaatg caagagctat gtatatgcaa gctatgagaa tgtcaaatat gacggagttt
     5821 gaagaattaa aaaattatga gggtgcaatc aaggaactta aacttataga gaaaatcaaa
     5881 aatggttcct ctgttattaa agaagaggca actaaaaaga aagaagagtt aactaaatta
     5941 gaaaaagaac aaaacgaagc agaggaacaa agaaagaaaa aggcaaaaga tacggctgga
     6001 gaagataggt atagagttga atcagatgca aggaaatctt cttatagtgg aagtagtaaa
     6061 aataataaat ctagttctaa aaataatgat aaaaaatcag atagtaataa gtctgacaat
     6121 aataacagtt cttctcaaaa tcaagaaaag ccaaataata gtaatccaac accagcacca
     6181 actccacaag atactactgg tggttcagat agtggtggaa attctcaaaa ctcaaataat
     6241 caataagtat aaaatatatg cataaattta atgaacaaat tatatattat aaaaaatatc
     6301 tcttagcaat tttttcttac taagagatat tttttgagaa ataatttaat aaatagcagt
     6361 taaattttaa actaaaaatt aaagtcacag tagagataag ctattgtaaa cgattatcta
     6421 tatatttatc aacataagtt tttacaacag cagctattgg tactgaaaga aaaactccta
     6481 tgaaaccaaa tagagattgt cccaccaaaa tagcagatat tacccatata gctcctatac
     6541 caacttgatt acccataact tttggttcta taaaatttcc atctaattgt tgcaatataa
     6601 atagaaatgc aataacccat atagtttttt gtggactata taatatagtc atggctattg
     6661 gagggattgc accaataaaa ggaccaaaat agggaattat attacctaag aatattattg
     6721 aagctaaaaa taaagcattt tctattctga taacaaattg aaatcctaaa aatgcaatta
     6781 taccaataat aagagagtct aatattctac caagtaaata atcatagaaa atatcatgag
     6841 tagtcctaaa aaattctata atgttatctg ccttgtttct agcagtagaa gaatatagaa
     6901 atctcttaaa tcccaatgca attttttctt tgtcatatat aatgtatata gatagtataa
     6961 atcccattgt catattaaat agcatagaag atatactaag tgtatagatt aagaagtttt
     7021 taggaatttt accaacaaaa ttacttgctt cttttaataa attatctaaa ctatgttgta
     7081 tatgaggaat taaagttttt agaaaatcta tgtttgacat atttttttct ataaaactat
     7141 ctatataatc agtatacata ggcatttcat ttatcaatct atttaatgta ttagctattg
     7201 atggaattaa taacctaatg gcaaaggcta aaataaaacc tataaatata taagataaaa
     7261 aaatggctaa aagtctatgt gctttgaatt ttacctcaaa aagcatcaca agtggattta
     7321 aaagtaaagc aagtaaaata gctaataaaa atgggctaaa aaactttaag aatccttcaa
     7381 tactagatat aaagtctgat ggagtattaa taaatttaaa aaataaaatt ccaataaaca
     7441 cgactattaa atagtatttt atattttccc ttgaaataat aatcacttcc ttaaaatagt
     7501 gtttagtcag ctattacata ttaatagtat gtgaaaagaa attacaatta tttaggaaaa
     7561 ttaaataatt tttttgcttg tttttaatta aggtctagta tagtataata atgtagcaaa
     7621 attaacattg ataaaaatat taaacacaca taatttaaga taaggaagga ttaagtatga
     7681 gaattaataa gtatatagct tcttgtggaa tagcatcaag aagaaaagca gaagaaataa
     7741 ttcttggagg aagaataaag gtaaatggaa gtgtagtaaa agagttatct tttaatattg
     7801 atgaagaaaa agacatagta gaatttgata ataaaaaagt aaaaccaagc gaaaattata
     7861 tatatatagt attaaataaa ccagagggat atataactac agtaaaagac caattcaata
     7921 gacccagtgt aatagacata cttaaagatg taagggaaag agtatatcca atagggagat
     7981 tagattatga aactagtgga ttattaatac ttactaatga tggcgattta acatataagc
     8041 ttacccatcc aaagcatgaa attgataaaa cgtatgtggc aagtgtgaag ggaattataa
     8101 gtgatgatga gataagacaa ttcgaaacag gactaaaaat agaagattac acaacggcac
     8161 ctgccaaaat aagggtaact aaagaaaata aagaaaaaaa ttattcagtg tgtgaaataa
     8221 ctatacatga gggtagaaat agacaagtaa gaaaaatgtg caaagctata aatcatccag
     8281 ttttaaattt aagaaggatt tctgtgggga aaatagtatt aaaagacacc aaagttggag
     8341 aatatagata cctaacagaa gatgaaataa aatatttaaa atcaataaaa taagataaaa
     8401 ataaattgag aaaaaatgaa ataaagttaa tagtagggag aaatagtatt atggcatatg
     8461 caaagtattt aactaaaatt acagatataa ataaatttta tttagaaaat attataaatg
     8521 aaggtgacat agtaattgat gctactatgg gaaatgggta tgatacaagg tatctagctg
     8581 agaaggttgg agaaaagggc tttgtatatg cttttgatat acaagaagaa gcaataaagt
     8641 ctacaagaaa gaaactagag aaagaaggat atacagatag agtaaaattg atatttgatg
     8701 ggcatgaaaa aatgaatcaa tatataaaag aagaagtttc atgtgtttta tttaatttgg
     8761 gttatcttcc aagagcgaaa cattcaatta taaccaaacc agatacaact ttaaaggcta
     8821 taaagttaag tttagaaatg ctaaaagaaa atggagtaat aagcattgcc atatatacag
     8881 ggcatgatgg aggtcaagaa gaaaaagatt gcatatataa ccttgtcaat aatttaaatc
     8941 aagatgaatt taatgtttta gaatctaagt ttttaaatca agtcaataac ccaccacaat
     9001 tgattttaat tgaaaagaaa aaagcataat aaatactaaa aaacttgaca agattgtatt
     9061 aagagttaat atatatagtg tgacctatta aaaatacttt aaataataag tatacaacag
     9121 cttattctca aaacaaaata tacatctaaa tatgcaaaat aagatagata acttttaaat
     9181 gagtaaaaat tgaaaaattt aaaagaaata ttggataaat attaaaaaat gcaaggaaag
     9241 atggtataat aaatttaatc gaaaatacta tgtgaagtat aaggtacaga aaagttagaa
     9301 ttttatgagt ttaagaagct atgataaaaa ctaagatgaa attgagataa aaaataaaat
     9361 ttttagagaa aaagacagtg ttaataaaac aaaatagttt atataactaa aaggagaaaa
     9421 aaatggaaga aacaaatgaa atgaaggaca gtaaacattt gatatcaaat atacaatctc
     9481 aatatacgag attgagtaag ggtcaaaaac ttatagcaca atacatttta aataattatg
     9541 ataaagttgc atttatgact gcatgtaaat tgggtgaaac tgtaggagta agtgagtcta
     9601 cagttgtaag atttgcaaat gcattaggtt atagtggata tcctaaatta caagctgcac
     9661 ttcaagaact cataaaaaat aaactaacta cagtacaaag agttgaaatg gcacatgatt
     9721 attccgatga ttttgcaata ctaaacaaag ttttaaaaag tgatatagat aatataaggt
     9781 ctacactaga agaaattgac gagagagcgt ttaaagaagc ttctaacaaa cttttaagag
     9841 cgagaaaaat atatatactt ggaatgagga gttcttttgt agtagctcaa tatttaggtt
     9901 tttacttaga tataatactt gacaatgttc atataattag aatggatatg ggagatgctt
     9961 ttgagcagat tgtaagaata aatgaagaag atgtaatagt tgctattagt ttccctcgtt
    10021 attctaagaa atcttatcaa attgtcaatt atgctaagga aaaaggagcc catgtaatat
    10081 cattaacaga tagcttattt gcgccagtag catcacttgc agataacact cttctagtta
    10141 agagtaatat ggcttcattt gtagattctc tagttccagc tcttagtatt tcaaatgccc
    10201 ttgctatatc tgttgggatg aaggaaaaag aagatattaa acagcatttt gatgatttag
    10261 agcaaatttg gaaaagatat tctgtgtatg agtaaagttt gtatattaat aaggctatga
    10321 aattgagaat catagcctta ttagtaaata ctcgattatc acttaaaaaa taaattttcg
    10381 tttaaataat acaaataaat atttcgagac tcaaaacata aagatgtatt ctgaatacat
    10441 ctttactacg tcaactaatt tattaaagaa agcatttcca ttcatggaga gtgctttttt
    10501 tatttctcta aacaatatca tttccttaat taacaattat aaaattaatt ttatttgtat
    10561 ttaagtatat aatatcttat taaaattata ttaaaatatg tatttctatc ttttttgttc
    10621 actataatat gtgtctttta gtattttatt ttaactttga caaagaagtt gaaaaattta
    10681 aaaatcatag aattagtagt taggtatgaa atcttgattt tttaagtagc catttttggg
    10741 gaaagggaaa cttttttaga aatataatac tttataaaaa taaaataaag tttttataaa
    10801 tattaagaca tagaatataa agcttttgta ataatgtatt taccaaaata atacaatata
    10861 tcttatatta taactatata aattatataa atcaatactt aaacatacca atagaggcat
    10921 tttaaaaaaa acataaattt ataatataaa attgttttaa gctccctttc c
//
LOCUS       DAEORZ010000138        10735 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEORZ010000138 DAEORZ010000000
VERSION     DAEORZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.140"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..550
                     /locus_tag="KRH39_003090"
     CDS             <1..550
                     /locus_tag="KRH39_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG8512975.1"
                     /translation="LVPPTHPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLV
                     GETREGVLAPIQALLDEMMKEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEE
                     DEFVQAAYKGLKEAGIDSEITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYI
                     EQEQLFIGTEGYYGILKSVYGK"
     gene            683..2053
                     /gene="hydA"
                     /locus_tag="KRH39_003091"
     CDS             683..2053
                     /gene="hydA"
                     /locus_tag="KRH39_003091"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG8512976.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILSINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            2071..3477
                     /locus_tag="KRH39_003092"
     CDS             2071..3477
                     /locus_tag="KRH39_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8512977.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            3549..3995
                     /locus_tag="KRH39_003093"
     CDS             3549..3995
                     /locus_tag="KRH39_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG8512978.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            4034..4828
                     /locus_tag="KRH39_003094"
     CDS             4034..4828
                     /locus_tag="KRH39_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG8512979.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            4818..7109
                     /locus_tag="KRH39_003095"
     CDS             4818..7109
                     /locus_tag="KRH39_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG8512980.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEEPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            7110..8423
                     /locus_tag="KRH39_003096"
     CDS             7110..8423
                     /locus_tag="KRH39_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG8512981.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            8459..9544
                     /locus_tag="KRH39_003097"
     CDS             8459..9544
                     /locus_tag="KRH39_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG8512982.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            9774..10697
                     /locus_tag="KRH39_003098"
     CDS             9774..10697
                     /locus_tag="KRH39_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8512983.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMIVMKEDMSEVRQEINIMKEDISEVRQ
                     EMTVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEI
                     NVMKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYG
                     IEIKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIK
                     EDIAEIRRDLNLVELATSKNWSDIVKLKSVK"
BASE COUNT         4298 a         1145 c         2184 g         3108 t
ORIGIN      
        1 attagtacct ccaactcatc cagttttagg tgatggaata ttagttttaa cagatataaa
       61 atcttctcca tatccaggag catcagtagt gccagattat tgcaaagcta cttttgatag
      121 aagattatta gttggagaaa caagagaagg cgtattagct ccaatacaag ctttattaga
      181 tgaaatgatg aaagaagacc cagaattaaa tgcaaaagtt tcttatgcgg ttgaaaaagc
      241 tgattgctat acaggaaata ctatagaaag tgaaagattc ttcccaggtt ggttatatga
      301 cgaagaagat gaatttgttc aagcagcata taagggatta aaagaagctg gaatagattc
      361 agaaataact caatactcat tctgtacgaa tggaagtcac tatgcaggtg aagctggaat
      421 aaaaactata ggatttggtc cttctaagga aaatcttgct cacacaatag atgagtatat
      481 agagcaagaa cagttattta taggaacaga aggttactat ggaatattaa aatctgtata
      541 cggaaaataa tatttagttc acaataaatt atttaaatat agtctagagg tgctgaatat
      601 agcaccttta gaaaatacaa ttttaaataa agttttacaa tacttagaaa atactttaaa
      661 gcttaaaagt taggaggtat atatgggaac aatactaaga ggtggaacta taattacctc
      721 tgataagact tatataagtg atttaaggat agaagatgaa aagatagttg aaataggaaa
      781 taacttagaa ataaatggtg ataaggttat tgatgctaca ggtaaaattg taattcctgg
      841 tggaatagac acacatactc attttgatat gaatgcaggt tctattacaa ctgcagataa
      901 ttttgaaaca ggaacaagag cagctatagc tggaggaact actacaatac tagattttgc
      961 agaagccaat gagggggaaa atctactgca aggtgtagaa gcatatcata aaaaagcaag
     1021 tggaaattgt tattgtgatt atggttttca tatgacaatt acatgtttaa ataaagatac
     1081 atttaatcat atggaaaaac ttatttcaga tgggatagta tcttttaaga tgtatatggc
     1141 atatgatggc atgaaagtag atgatggaac aatatacagg gttttaaaga aagcaaggga
     1201 tttaggttgt atagttggat ttcattgtga aaatggggat ttactagatg tattgataga
     1261 agaaaatata caaaatggta atttggagcc aaaatatcat ccacttacaa gacctaacat
     1321 agttgagaaa gagtctgttt caagacttgc tgatataact aaattatcta attcaaaatc
     1381 ctatgtagtc cacttaagtt gtagggagtc acttgaaact gtaaaaatgg ctagagaaaa
     1441 aaacatagat atgatagtag aaacatgccc acagtatcta ttattagaag acaatttata
     1501 taataaggat aaatttgagg gtgctaagta tgtaatgtct cctcctttaa gaaaaaaaga
     1561 agacataaat tacctttggg aaggattgtc tagtggagat attcaaacag ttggcacaga
     1621 ccactgttca tttaatttta aggggcaaaa agacttggga attgatgatt tcagtaagat
     1681 accaaatgga gcacctggtg ttgaacatag gttagcactt ttatatacat atggggtctt
     1741 agaaaataga ataagtgtaa ataagtttgt ggaagttact tctacgaatg ctgcaaaaat
     1801 atttggaatg tatcctaaaa aaggtgagat agctgtagga agtgatgcag atatagtcat
     1861 tttaagtatc aataaagaag aaacaattag ttataaaact caaaaacaaa atgtagatta
     1921 tactccatat gaaggattta aggttaaatg taaagttgaa gatgtatttt tgagaggaaa
     1981 tcatgtagta caaagttgta atgttaaaga acatccaaca ggtcaatata ttaaaagaaa
     2041 aattactaaa taatgcgggg ggaaataaga atgatggata tacttattaa gaatgccata
     2101 atagtaactg tgaataaaga gagagaagtt atttttgatg gggcattagt agtaaaagat
     2161 aacaagattg ctgatattgg aaattctaaa gagatagaaa gtaaatatac tgatgttaaa
     2221 aaaattatag atgctaaagg taaagtttta ttcccaggtt ttataaatac acataatcat
     2281 ttattccaaa ctttattaaa aggattggga gatgatatgg tacttaaaga ctggttagaa
     2341 actatgacat ttccagcagc taactattta gagccaaaag atacttatga tgctgcaatg
     2401 cttggatgta ttgaaggact tagaagtggt ataacaacaa tggttgacta catgtatcct
     2461 catagtaagc caggtctttg tgatggaata atagatgctt ataaagaatt aggaataaga
     2521 ggaatacttg gaagaggatg tatgaataca ggagctcaat ttggagttca tcctggaata
     2581 atgcaagatg tagaaacggt tgaaaaagat gtaagaagat tgtttgaaaa acaccataat
     2641 acagaaaatg gaagaatcaa aataggagta gctcctgcag caatatggtc aaattctcaa
     2701 gaaatgcttg aaatgctatg gagagtagta aaagaatatg atgatgcttt atttacagta
     2761 catatatcag agactccttt tgatagagaa gcagcaaaag aattgcatgg gcaatatgac
     2821 atagatgttt tagaaaaatt aggaatactt ggaccaaatg tgcttatggt acattgtgtg
     2881 tatctaactg aaaaagatat ggaacttact aaaaaatacg atatgaaagt gtctcataat
     2941 acagcaagta atatgtactt atcttcaggt gttgcaccag taccagaaat gcttaaaaaa
     3001 ggaataacag ttagtttagg agtagatgga gccgctagta ataactctca agatatgctt
     3061 gaacttatga aattaacagc acttcaacat aaagttaata agtgtgaccc attagcaatg
     3121 tcagcagaaa aagttcttga gttggcaact atagatggag caagagctat tggaatggaa
     3181 gatgagatag gttcattgga gataggtaaa aaagcagact tactaatatt taatccaatg
     3241 ctttcaccaa aagcgatacc tatgcataat ccggtatcta cattagtata ttcttctagt
     3301 atgaaaaata tagagagtgt tatagtagat ggaaatataa taatggaaga ttcaaaaata
     3361 ttgacagcta atgaagagaa agcattaaaa gatgctcaag atacagctga gagactttgt
     3421 gtaagaggaa ctataaaaaa tagaatggaa ggtcataaat ggaatagcct ttattaataa
     3481 atgtaattta ttcatagaaa agtatagtaa aactttagct taaaaatatg agttttgagg
     3541 tgatttattt gattatcaaa tgtaccataa ataataaaga aaaagaatta agtataagac
     3601 cagatgattt tttagcagat acattaagag aaaatggata tgtaagtgtt aaaaaagggt
     3661 gtgatacagg ctcatgtggg ttatgtacag tgatggtaaa tgacaaggcc gtgctttcat
     3721 gtaattatct tactgctaga gtagacggtc agcatattac aacacttgag ggtgtcttgg
     3781 atgaagcaaa gaagtttgga gattttcttg cagacgaagg ggctgaccag tgtggatttt
     3841 gtgctcctgg atttataatg atggttttgg caatgaaaag agagcttcaa aatccaacag
     3901 aagaagaagt tatagattat gtaaatggta acctttgtag atgtacagga tataaaggtc
     3961 aattaagagc aatacataaa tatttaaaca cttagacttt taagtgaaaa ttaagttgat
     4021 atggggagag agtatgataa ctataaagga atatgtagtt ccaaaaagtt tagatgaagc
     4081 ttatgaactg ctaatatcaa gaaaaaacaa cataatactt ggaggatgtg gatttttaaa
     4141 actaggtagt aaaaatattg gctcagcaat agatttaaaa gacttagctt tagattatat
     4201 aaatgaaact gatgatagca tattaattgg agcagacaca agtttaagaa ctttagaatt
     4261 aaataaggtt attaaaaatt actgtaatgg ggtaatttca aacgctgttt ctaatatagt
     4321 aggagttcaa tttagaagtg gagctagagt aggagctagc gtatttgcta agtatggatt
     4381 ttcagattta ataccttcat tactagtggt agatgcaaag gttaagttat ataaaaaagg
     4441 ggttatgaac ttagctgatt ttttagaaag tgagcttgaa aaagatatat taatagaagt
     4501 tatattacct aaaaaagatg ctataggtgt atttgattct ataagaaaat gtacaggtga
     4561 ttttgcagta ttaaatggag caatgttaaa agaaggaagt atttataaaa ttgcaatagg
     4621 cgctagacca agaagagcaa aaattgccta taaagcaagt gaaatattaa gtttagaaaa
     4681 tgatatagaa aaagctggtg aagtatcaag tgaagaactt tcatttggaa gtaatataag
     4741 aggaagtaaa gaatacagaa aagatatggc aaaagcctta gtagtgagaa tgtataatag
     4801 tattggaggt gagtgtgatg gtaaataaag aggtaagaaa aatagatagt gaagccattg
     4861 ttactggaaa accaatatat acagaagatt taatttttca taaagatgtt ttaacaatta
     4921 agcttcttag aagtccacat gcttttgcga agataaaaaa catagatata aaaaatgcat
     4981 taaaggtaaa aggtgtagta aacatattca cttacaaaga tgttccaaat tatagatatt
     5041 caatggttgg agagtcatat ccagaagcat cacctcatga ccaattaata cttgaagacg
     5101 tagttagata tgtaggagat gaagttgcta taatagccgc tgaagatgaa agggctgcta
     5161 ttaaagctat gaaacttata aaagttgaat atgaagtaat gactcctata ttggatgcaa
     5221 gagaatcgga aggaaataaa attttaatcc atgaagaaga tgatttgttt tgtccatttg
     5281 attttggatt ggatgctaca aaaaatattg tatcgagaga aaaaatgggt aaaggggatg
     5341 tagaaaaaga gtttaaagaa agtgatgtaa tagtagaaca ttcttatatg actcaatcac
     5401 aagcccattg tatgatggaa actcatagag cttatacgta tatagatacg aatggcagat
     5461 tagtagtaac ttctgcaaac caatcagctt atcatatgag aagacaggtt tctaaagcac
     5521 tgggaatacc tctttcaaaa gtgagagtaa taaaaccaag aataggtgga ggttttggtg
     5581 gaaaaaacat agctgtaaca gagatatatg catcatttgt aacttggatg acaaaaagac
     5641 cagcaaaact aatttatact agagaagaaa cttttgcaat gactaatact agacatcaga
     5701 tgttttttga tataaaaatt ggggcagata aagaaggaaa cataaaagct atagatatga
     5761 aagcattgaa caatacaggc gcttatggtg ataatggacc ttcagtttgc tctgaatcag
     5821 gtcataatgt acttccaaca tataataatg tacctgcaat aaaatttgat gggagaactg
     5881 tatatactaa tttagttcca ggaggagcat taagaggata tggagcaaca caaggtacat
     5941 ttgcacttga ttgtgcaatt gatgaattag cgcataaact taatatggac ccaacagaaa
     6001 taaagttaaa gaatattata aaaaaacaaa ctgaaggcgg aataatgaat tttccaataa
     6061 gaagttgtgc acttgatgaa tgtataatta gaggtaaaaa acttattgga tgggatgaaa
     6121 agtaccctgt tagggatatt ggcaacaata aggtgagggc tgttggaatg tctgtgggaa
     6181 ctcatggttc tggtatagcc aacatagaca tggcaatagt aaatatgagg atggatgaag
     6241 atggaactta taagttgttt tctgggtctt cagacttagg aactggttct gacacaatac
     6301 ttgcacaaat agctgcaaaa gcattgaata catctattga tagaatttcc gtttatacag
     6361 gagatacaga tatgtgtcca tatgatactg gtgcatacgc ttcttgtact acttatgtaa
     6421 ctggaaatgc tacaataaaa tctgctgaaa gtttaaaaca aaagatatta aaagtagcac
     6481 agaagaaact cgatttacca atagatgatt taatactata tgaagacaga gtttgttata
     6541 gtgaagatgt agagaagttt gttttgttaa gtgaattagg aaaagaatct gttggtggtg
     6601 gaaatcaaga agttttatat tcatctgaat cttatggtat acaagagagt gctaaaccat
     6661 tcttagcagg atttgctgaa atagagttag ataaaacaac aggggagttt aaagtaataa
     6721 attatgcttg ttctacagat tgtggtaccg ttatgaatcc tgcacttgct agaatacaag
     6781 ttgaaggtgg tgtaactcaa ggtatagggc ttgcaatgta tgaagaacct agacatggtt
     6841 ctgatggaaa gcttcaaact tctaatctat tacaatacaa agttccaaca aaaaaagata
     6901 taggaaacat aattgttgat tttgtagata attatgagcc aacaggtcca tatggagcta
     6961 aatctttagg agagatagtt attcatacac ctgcaccagc aatagcaaat gcaatataca
     7021 atgccacagg agttcgtata agagaacttc ctataacttc tgagaaagtt tatatggcca
     7081 tgaaaaaatt aaatcaggga ggtaaataaa tgattgctat taaaagtaaa tacttaatat
     7141 cttctgctga tgaaatatat acaaattctg ctgttgtagt agaaaatgac attataaaag
     7201 atatattgcc aaatgaagaa gttgaaataa aatataaaaa tattgaagaa ataatagata
     7261 aatcagatgc tataataatg ccaggattcg tcaatggtca tatgcatcaa tatggagttc
     7321 tttctagggg aataccggca aatgtacatt ttactgactt tgaaggtttt ttaaatgact
     7381 attggtggcc atttatagaa aatagaattg gattaaaaga agtgaaggca acaactaaag
     7441 catcagcaat agaacttata gaatctggtg tggtagcttt ttgtgatacc ctagaagctc
     7501 caaatacaga agaaggaact ttaattgagc aaggaaaaat actagaagaa ataggcatga
     7561 aagcgatttt atccttagaa agttgtgaaa gaataagctt tgaaaatgga cttagatgtt
     7621 tagatgaaaa ttctaattta ataaagtgga gtagagaaaa tagtaaatta ataaatggaa
     7681 taatgtgtac acatacaagc ttcacatgtt ctgatagatt tattaagaaa gctaaagaag
     7741 atgcaaaaaa attaaatgca ccaatgcaat ttcatttatg tgagagtata tatgagccaa
     7801 actatgctga aaaacacttt ggcaaaaaag cagttgacta ctataatgat ttagatatat
     7861 tggatgaaac tgtattagca tctcagtgtg taaaagtaaa tgatgaagaa atagatatat
     7921 taaaagaaaa aggtgtaaaa gtggttcata tgcctcttag caactgtgaa gtaggtggag
     7981 gtttttctcc agtaccaaaa atgattaaaa aaggcataaa agtggcactg ggtactgacg
     8041 gatatataaa tgattttttt gaagttatga gaggtgcatt tttaatgcat aaatcagttg
     8101 aagaagatgc atcagtaatg ccagcaaatt tagtatttag aatggcaaca gagcatggtg
     8161 cttatgtatt aggacttcaa aattctggta aaatagcagt tggtaataag gctgatataa
     8221 tagttatgga agatgaattt aaaactcctg tgactcttga taatatattt gaccagatag
     8281 tagttcaggg taaaaaagaa tttatatcaa atgtatacat agatggtaga catattttaa
     8341 aagaaaaaca gttagttgat ttagataaaa aagctattgt taaagaaatg aaagaagttg
     8401 cttgtgaatt ttggaaattt taacgaatgt aaatatatat gaaaagggga agcagataat
     8461 gtataatttt ttaggtaaag aattaaaatc accacttata ataggttcag gaccattaac
     8521 atatagtgca gctggatgca agatattaag tgatgcagga gcaggggcag ttgtgactaa
     8581 gactataaga aaagaaagag ctataaatcc agcaccacat atggttagaa atacagctaa
     8641 tgctctttta aataatgaaa aatggacaga ttttgagcca gagcaatgga tagattttga
     8701 aataccacaa atgaaaagag atgggactgt ctgtatagct tcaataggtc atacaattga
     8761 agaaagtagc gaattagtag agaaagttgc aaatgcagga gctgacttta ttgaattagt
     8821 atcttatgac tatagagatt tgataccaat gcttaaagat gcaaaggaaa gagtaaatat
     8881 acctgtaata gttaaacttc ctcctatgat agatgagata ggcgattttg ctaaaaaatt
     8941 agaagaagca ggagcagatg caataactgc ttgtgactct gtaggaccag catttagaat
     9001 agacatagaa acagggcaac ctttattagg tggaaatgga ataggatatt taagtggaga
     9061 aactatcaag ccaataactt tacagagaat atatgaaata aaaaaacaag ttaatatacc
     9121 aataattggc ttaggtggat gtgtaagtgg agatgatgcc ttagaaatga ttatggcagg
     9181 tgctgacttt gttggtatat gttcagtagt aattttaaaa ggcgcacaag taatatctaa
     9241 aatacatgat gatttaaaat ctaatcttaa tagattagga tataatacta tagaaaatgc
     9301 ttgtggtata gtacataatc atatgggaga tgttaatgaa agacttagct ttgagtttac
     9361 ttatgatgaa gaaaaatgta ctaaatgtaa gatgtgcgag agagtttgtg catatcaagc
     9421 gagaaaattg aatgataaga tggaattgaa tgaagaagag tgtagatatt gtggactttg
     9481 tatatcagtt tgtaaaccaa aagcacttgg aagaaaaatt tcatgttcag agctgaatgt
     9541 atagattatt ttataaaaaa caaagtttta aataaaaata agaaaggcta tctcaaagag
     9601 ataagccttt tttgttgttg accttatttt tgtttatttg tagttatttt agatattaat
     9661 tagttttgaa gtaataaagt gaatcaattt tgtttaatta aaattttaat aattctgata
     9721 tatatgttaa cattgtatag catttgtgta aaatgttata ataattaata gggatggtga
     9781 tgcaagtgca agagaagatt ttagagttat tatatactat aaatgaagat gtagttagca
     9841 taaaaaatga tatgtcggaa gtaaaacaag aaataaatgt gttaaaagaa gatatgtcgg
     9901 aagtaaaaca agaaatgact gtaatgaaag aagatatgtc ggaagtaaaa caggaaataa
     9961 atgtattaaa aggagatatg tcggaagtaa aacaagaaat gattgtaatg aaagaagata
    10021 tgtcagaggt aagacaagag ataaatataa tgaaagagga tatatccgaa gtaagacaag
    10081 aaatgactgt aatgaaagaa gatatgtcgg aagtaaaaca ggagataaat gtattaaaag
    10141 aagatatgtc cgaagtaaga caagagataa atataatgaa agaagatatg tccgaagtaa
    10201 gacaagaaat gaccgtaatg aaagaagata catcagaggt aaaacaagag ataaatgtaa
    10261 tgaaaaaaga catgtcagaa gtgagacaag aaataactgt aatgaaagaa gatgtttccg
    10321 aagttaaatc agaagtaaac tttatgcaaa ataaaataaa taacattaat gaagatatga
    10381 atggaattaa ggacgaggta tccatagcaa atgaaaaatt atatggaatt gaaattaaga
    10441 ttgatagtct agaatgtgaa gataaatcaa tgaaagagat acagatagaa cagaataata
    10501 ttttaggaag tttattacat aattctgaaa taaataaagc tactcatgat aatatagaac
    10561 ataatatagc atatataaaa ggtgacacta attctataaa agaggatata gctgaaataa
    10621 gaagagactt aaatttagta gagttagcca ctagtaaaaa ttggagtgat attgtcaaac
    10681 taaaatcagt aaagtagtat tttaattcac taatttatca tccacaaact aagaa
//
LOCUS       DAEORZ010000139        10644 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEORZ010000139 DAEORZ010000000
VERSION     DAEORZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.141"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..1649
                     /locus_tag="KRH39_003099"
     CDS             <1..1649
                     /locus_tag="KRH39_003099"
                     /inference="COORDINATES: protein motif:HMM:NF012706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBG8512984.1"
                     /translation="IAFVLASTFIFGALTGCGKKKEAKSTTPKDKIVYALWSSPTGIF
                     NPLLSDTTYDDAVISLTYNSLLKFDEKLNPQPDLAESYKISDDKLSITFKIKDGVKWN
                     DGKTLTAEDVAFTFTSIADKDYQGARYGDVEKLVGASEYKERKADGIKGIEVIDNNTI
                     KFTFKEVYSPALTNIGGMGIIPKHVWSEVPVSQWKEQKDLLTKPVGSGPYKVASFTEG
                     QDVQLESFDEYFNGKPKTKKFIFKVSNEDTTQVELLDGTVDVADVSNLKKKDLEEIKS
                     EGMNIKSYSNNMIQYMGLNLREKKFQDKNVRQAFMYALDRKSLVDKLLEGNGEVVNTP
                     MLPSSWPYPEASKLNDYKYSVEKAKELLKKAGYEDKNNDGIVEDKEGNKLTAKLSYPT
                     GNKLREQTAPIIQESFKQIGVQVELEGLEFSALMDKVVANHDFDLYLMGNNLAVDPDP
                     TPYWKSSSASDEKGVSAWNISSFKNEEADKLMDEGLKVFDQKERKKIYENFAVLMNDE
                     VPWTYLYAQNIVKASNPKLKNFNPSTFRDFSDVEKWYIEN"
     gene            1728..2690
                     /locus_tag="KRH39_003100"
     CDS             1728..2690
                     /locus_tag="KRH39_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512985.1"
                     /translation="MHKYIIKRLLTTIIILFGISVIIFALINMQPGNPYSTMIDPSVP
                     PEVVKEMLNKIGYYDPIHIKYIKWISRAITGDLGYSIYFGQPVLSVINGRIWNTILLS
                     VFALIISIVLGISTGIISAIKEKTLFDNIITVISFMGVSIPAFFLGLILIKIFSFDLK
                     ILPASGMKTLGESYTGLEGIFDLLKHMLLPGLVLAILQSTAFIRYTRSSMLEVLGNDY
                     IRTARAKGLTKNKAIFKHGLKNALIPIVTIICLQIPFLFSGALLTETVFVWPGIGRLN
                     YDAVLNRDYPLIMGILMILSIIILLSNLIADILYAIIDPRIRYK"
     gene            2702..3529
                     /locus_tag="KRH39_003101"
     CDS             2702..3529
                     /locus_tag="KRH39_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8512986.1"
                     /translation="MLENIKKNKLIIIGVSILSILIFISIFGKYFTKYDISSIDLFSI
                     NSSPDSEHIIGTDDLGRDILARLLYGGRVSLCVGIFATLIQVSIGIILGTIAGYFGKF
                     VDSFIMRIVDIVMCFPFFVVAIALAAIVGANITNLIIIIAVLSWTDVARIVRSQVLSL
                     KEREFVTASKAIGFSTFDIIIKHIIPNILPAILVAMTLSMANAILMEASLSFLGMGVK
                     PPMPSWGNMLTAAQNMRTLRSEWWIWMPPGFMIIISVLAINFIGEGLRSMLSPKERE"
     gene            3535..4479
                     /locus_tag="KRH39_003102"
     CDS             3535..4479
                     /locus_tag="KRH39_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512987.1"
                     /translation="MLLEVKNLNVEINNKVIIDDISFCVGEGEIISLVGESGSGKSLT
                     SLAIMGLLPEKAKISGEIKLDEKNLVSLTEKEICKIRGKQISMIFQEPMTSLNPLFNI
                     ESQIGEVISIHEKTATKNIRNRVIDLLEKVKISNPQNILKKYPHELSGGMRQRVMIAM
                     AIALSPKLLIADEPTTALDVTIQDDILNLLKEISFEMGMSIIFITHDLDVIAEIANKV
                     IVMYLGKVFEKALVDEFFDNPLHPYSKGLMLSRTNNILESGRMFSIKGIVPNILDIPK
                     GCRFNTRCDECFDKCIENSPTLIDVNKNHSVSCFIYQK"
     gene            4494..5483
                     /locus_tag="KRH39_003103"
     CDS             4494..5483
                     /locus_tag="KRH39_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512988.1"
                     /translation="MNQDYILKIENVKKYYPIDNSIFLRKISFIKSVDGVSFNIERGK
                     SLGLVGESGCGKSTISRCILNLIEPTYGRVEFQDKIIYDVENKINIDKKEMIKLRKDM
                     QIIFQDPYSSLDPKMTIGKIISSGIKKHQIAQGEYALNLAKDMLNLCGLDESYINRYP
                     HELSGGQRQRIGIARALSLNPKFVICDEPTAALDVSVQAQILNLMLDLKDKLNLTYLF
                     ISHNLNVVKFFCDEIAVMYLGVIVEKAPSYSIYNKPFHPYTKGLISSIPKTHPNIKKE
                     RILFKGQLSSNSEDFKGCRFSTRCPFSKKMCMSKTPVYKEVEKNHFVACHLYE"
     gene            5628..7511
                     /locus_tag="KRH39_003104"
     CDS             5628..7511
                     /locus_tag="KRH39_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBG8512989.1"
                     /translation="MSNKDKENMTLSNQITESTQETLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWYGGTNIIMNKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYVDIPKVPNWVKDTVWYQIFPDRFANGDPSIKPEGVDEWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPALGDKDTLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKDWFYIKDMSKVDTPVEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNVDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNREIFNFMKKIIRLRKENKEFRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKDIILTLPDYLANKKVKDLYEEEIVDLKEELILEKYKFI
                     ILK"
     gene            7736..8416
                     /gene="pgmB"
                     /locus_tag="KRH39_003105"
     CDS             7736..8416
                     /gene="pgmB"
                     /locus_tag="KRH39_003105"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG8512990.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWKKLAHKIGINIDTKFNESLK
                     GISRMESLDRILEFGNKKDLFSKEEKLKMAEEKNNYYISSINDITSSDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIANSAKCKNNKPHPDIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            8413..>10644
                     /locus_tag="KRH39_003106"
     CDS             8413..>10644
                     /locus_tag="KRH39_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_097546124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG8512991.1"
                     /translation="MKCLFSNIDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAISHESDYNYCENSITMDALKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKEYNECIDRNNVIREEIEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDRILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWDETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKI"
BASE COUNT         4269 a         1111 c         1906 g         3358 t
ORIGIN      
        1 taattgcatt tgttttagct tcaacattta tatttggtgc tcttacagga tgtggtaaaa
       61 agaaagaagc aaaatcaaca actccaaaag ataaaatagt atatgctttg tggagttctc
      121 caacagggat ttttaatcca ctattatcag atacaactta tgatgatgcg gtaataagtc
      181 ttacatataa ttcattactt aagtttgatg aaaaattaaa tccacaacca gatttagctg
      241 aaagttataa gatatcagat gataagttaa gtataacctt taagattaaa gatggagtaa
      301 aatggaatga tggaaaaact ttaacagctg aagatgtcgc ttttactttt actagtatag
      361 cagataaaga ttaccaaggt gctagatatg gtgatgttga aaaattagtt ggagcatctg
      421 agtataaaga gagaaaagct gatggtataa aaggtataga agtgattgac aacaatacta
      481 taaaatttac ttttaaagaa gtttactctc cagcacttac aaatattgga ggaatgggaa
      541 taatccctaa acacgtatgg tcagaagtac cagttagcca atggaaagaa caaaaagact
      601 tgttaactaa accagttggt tcaggtccat ataaagttgc atcatttaca gaaggtcaag
      661 atgttcaatt agaaagcttt gatgagtatt ttaatggaaa acctaaaact aaaaagttta
      721 tctttaaagt atcaaatgaa gatacaacac aagttgagct tttggatgga acagttgatg
      781 tagctgatgt atcaaattta aaaaagaaag atttagagga aataaaatct gaaggaatga
      841 atattaagtc ttattcaaat aacatgatcc agtatatggg gcttaatctt agagaaaaga
      901 aattccaaga taaaaatgtt aggcaagcat ttatgtatgc tttagataga aaatcactag
      961 tagacaaatt gttagaagga aatggagaag tggtaaatac tccaatgcta cctagttcat
     1021 ggccatatcc agaagcttca aaactaaatg actataaata tagtgtagaa aaagctaaag
     1081 aacttttaaa gaaagctggt tatgaagata aaaataatga tggaattgta gaagacaaag
     1141 agggcaataa attaacagct aaattatctt atccaacagg taacaaatta agagaacaaa
     1201 cagctcctat aatacaagaa agttttaaac aaataggtgt acaagttgaa cttgaaggat
     1261 tagagttctc agcattaatg gataaagttg ttgcaaatca tgattttgat ttatacttaa
     1321 tgggaaataa tttggcagtt gatccagacc ctacaccata ttggaaatct tcatctgctt
     1381 cagatgaaaa aggtgtttct gcttggaaca tatcttcatt taaaaatgaa gaagcagata
     1441 aactaatgga tgaaggatta aaagtatttg accaaaaaga acgtaaaaaa atatatgaaa
     1501 attttgctgt gcttatgaat gatgaggttc catggactta tctttatgca caaaacatag
     1561 ttaaagcatc aaatcctaaa cttaaaaatt tcaatccgtc aacatttaga gatttttcag
     1621 atgttgagaa atggtatata gaaaactaga aaaaggtgat ttttaaatca ccttttttaa
     1681 ataatatctt taaagtgctt ataaattaag aaatagaggt gaatagtttg cataaatata
     1741 taataaaaag gcttttaaca actataatta ttttatttgg aatatctgtg attatatttg
     1801 cgcttataaa tatgcaacca ggtaatccct attctaccat gatagatcca tctgtaccac
     1861 cagaggtagt taaggaaatg ctaaataaaa tagggtacta tgatccaatt catattaaat
     1921 atataaaatg gatatcaaga gcaattacag gagatttggg atattctatt tattttgggc
     1981 aacctgtatt gtctgtaata aatggcagga tatggaatac tattttgtta tctgtttttg
     2041 cattaataat aagtatagtt ttaggaattt caactggaat catttctgca attaaagaaa
     2101 aaactttatt tgataatatt attacagtaa tttcttttat gggagtttca attcctgctt
     2161 ttttccttgg cctaatctta ataaagatat tttcatttga cttgaaaatt ctcccagctt
     2221 cgggtatgaa aactttagga gaaagttata caggattaga aggaattttt gatttactaa
     2281 agcacatgct acttccagga ttagttcttg ctatattgca aagtacagca tttataagat
     2341 atacacgttc ttccatgtta gaagttcttg gaaatgatta cataagaact gcaagagcta
     2401 aaggattgac taaaaacaaa gctatattta aacatggtct taaaaatgct ttaataccaa
     2461 tagttactat aatatgttta caaattccat ttttattttc aggagcgctt ttgactgaaa
     2521 ctgtatttgt atggcctgga ataggaagac ttaactatga tgcagtgtta aatagagatt
     2581 atccgcttat aatgggcatt ttgatgattc tttctataat aatactacta tctaacttaa
     2641 ttgcagatat attatatgca ataatagacc caagaattag atataaatag aggtgattag
     2701 gatgttagaa aacattaaaa aaaataaact tattataatt ggagtaagca tactttctat
     2761 attaatattc atttctatat ttggaaaata ctttacaaag tatgatattt cttctataga
     2821 tttatttagt ataaattcat ctccagattc tgaacatatt attggaactg atgatttggg
     2881 aagagatata ttagcacgtc ttctatatgg agggagagtt tctctatgtg taggtatatt
     2941 tgcaacatta atacaagtca gtataggaat tatacttgga acaattgctg gatattttgg
     3001 gaaatttgta gatagcttta taatgcgtat agtcgatata gttatgtgtt tcccattttt
     3061 tgtagttgca atagctcttg ctgctatagt tggagcgaac ataactaatt tgataataat
     3121 aatagcagta ctatcatgga ctgatgtagc aagaatcgta aggtcacaag tactttcatt
     3181 aaaagaaaga gaatttgtaa cagcatcaaa agcaatagga ttttctacat ttgatataat
     3241 aataaaacat ataattccaa atatattgcc agcaatttta gttgcaatga ctttgtctat
     3301 ggcaaatgca attttaatgg aggcatcact aagtttcttg ggtatgggag taaagccacc
     3361 aatgccaagt tggggaaata tgcttacagc agcacaaaat atgagaacat tgagaagtga
     3421 atggtggata tggatgcctc caggatttat gataataatt tctgtgttag caataaattt
     3481 tataggagag ggattaagaa gtatgttgtc tcctaaggaa agagagtaga gaatatgtta
     3541 ttagaagtaa aaaatctaaa tgttgaaatt aataacaagg ttataattga tgatattagt
     3601 ttttgtgttg gagaaggcga aataatttcc ttagttggtg aatctggaag cggaaaaagt
     3661 ctgacttcac tagctataat ggggttatta cctgaaaaag caaaaataag tggcgaaata
     3721 aaattagatg agaaaaactt ggtgtcttta acagagaaag agatttgtaa gataagaggg
     3781 aaacaaatat ctatgatatt tcaagaacct atgacttctt taaatccttt atttaatata
     3841 gaaagtcaaa ttggtgaggt aatatcaata catgaaaaaa ctgctacaaa aaatatcaga
     3901 aatagagtga ttgatttact ggaaaaagtt aaaatatcta atcctcaaaa tatactaaaa
     3961 aaatatccac atgaattaag tggtggaatg agacaaagag ttatgattgc aatggcaatc
     4021 gcactaagtc caaagctttt aattgctgat gaaccaacta cagctttaga tgtaacaata
     4081 caagacgata tattaaattt attaaaagaa ataagctttg aaatgggaat gtcaataatt
     4141 tttataactc atgacttaga tgttattgca gaaatagcaa ataaagttat tgtaatgtat
     4201 ttaggtaaag tgtttgagaa agctctagta gatgaatttt ttgacaatcc tcttcatcca
     4261 tattcaaaag gacttatgtt atcaagaaca aataatatat tagaaagtgg aagaatgttt
     4321 tctataaaag gtattgtacc aaatatctta gatataccaa aagggtgcag atttaacaca
     4381 agatgtgatg aatgttttga taaatgtatt gaaaatagtc ctactttgat agatgttaat
     4441 aaaaatcata gtgtaagttg ttttatttat caaaaataga aaggtatgtt aatatgaatc
     4501 aggattatat attaaaaatt gaaaatgtaa aaaaatatta tcctatagac aattctatat
     4561 ttttgaggaa aatatcattt ataaagtcag ttgatggagt ttcttttaat atagaaagag
     4621 gaaagtcttt aggattggta ggtgagtctg gatgtggaaa atctactata tctagatgta
     4681 ttttaaatct aattgaacct acatatggta gagtcgaatt tcaagacaag attatttatg
     4741 atgtagaaaa taaaataaat atagataaaa aagaaatgat taagttaaga aaagatatgc
     4801 agataatttt tcaagatcca tattcttcac tagaccctaa aatgactata ggaaaaataa
     4861 tatcaagtgg tataaaaaaa catcaaatag cacaaggtga atatgcttta aatctagcta
     4921 aagatatgtt aaacctttgt ggacttgatg agagttacat aaatagatat ccacatgagt
     4981 tgagtggagg tcaaaggcaa aggataggaa tagctagagc cttatctcta aatcctaaat
     5041 ttgtgatatg tgatgaacca acagctgcat tagatgtatc agtacaagct caaatattaa
     5101 acttaatgtt agatttaaaa gataagttga atttaaccta cttatttata tctcataatc
     5161 taaatgtagt caaatttttt tgtgatgaaa tagcagttat gtatctaggt gtcatagtgg
     5221 aaaaagctcc atcatattct atatataata aaccatttca tccatataca aaaggtttaa
     5281 tatcatcaat tccaaaaaca catccaaata taaaaaaaga aagaatttta tttaaaggac
     5341 aactttcaag taattcagaa gattttaaag gttgtaggtt tagtactaga tgcccttttt
     5401 ctaaaaagat gtgtatgagt aaaactcctg tatacaagga agttgaaaaa aatcactttg
     5461 tagcatgtca tttatatgaa taaatttaag tttaagaaaa gtgattgtct taaattagaa
     5521 aagtattact attatattta actattatat ctttttatta tgatataata gttaaataca
     5581 ttaacctatc tttaaatcat gcaaattatt agaaagaagg gataaagttg tctaataaag
     5641 ataaagaaaa tatgacttta agcaatcaaa tcacagaatc aactcaagaa actttaacaa
     5701 aagaagctat attacatata ccaatgagca attatgccta tggttatgat agagaaacac
     5761 tacatataag acttagaact aaaaaaaatg aagcgaaaag agtaatttta cgaataggag
     5821 accaatacgt gtgggataaa ggaggtgcag gaggaggaaa tttaaatgca tctggagttg
     5881 gttggtatgg tggaacaaat ataataatga acaaagaagt agaaactgaa ttatttgatt
     5941 attggatagc taagtgcaaa cctttaaata aacgttcacg atatggtttt ataatagaag
     6001 gtcaagaaga aaaactttta tttacagaaa aaagaataat agagttggga aatgaaaatg
     6061 atgaaaagga attatgtgaa ataggtaatt tttttggata tccatattta aattatgtag
     6121 atatacctaa agtgccaaat tgggtgaaag atactgtttg gtatcagata tttccagata
     6181 gatttgcaaa tggtgacccg tctataaaac cagaaggagt agatgaatgg ggagctattc
     6241 caaccagaga taattttaca ggtggagatt tacaaggtgt gatagaacac ttagattatt
     6301 tgagtgattt gggaataaac ggaatttact tttgccctat tacaattgga aaaaccaatc
     6361 atagatacga tacggtagat tatatggagg tagaccctgc attaggagat aaagatactt
     6421 taaaaaaact aatagaagaa gcgcataaga gaaatataaa aataatgttg gatgctgttt
     6481 ttaatcatat aggatattat tctaaacagt ggcaagatgt agttcaaaat aaggaaaatt
     6541 ctcgatataa agactggttt tatataaaag atatgagtaa agttgacact ccagtagaac
     6601 agatagatga aaaaaatata ccttatgaaa cttttgggtg tgaaaaatat atgcctaaac
     6661 ttaatacaga aaattcagaa gtaataaaat atttattgga tgtaggcaag tactggatac
     6721 aggaatttga tatagatgcg tggagattag atgtttcaaa tgaagtagac catgtttttt
     6781 ggagaaagtt tagagaagaa gtcaaaaaag taaaaccaga catttatata ttgggagaaa
     6841 tttggcatgg tagtttacca tggctaatgg gtgaccaatt tgattcagtt atgaattact
     6901 tgatgtcaga agccatgaaa aaattctttt gtacagatga gataaatgct gaagaattta
     6961 agtatatgat aaatgatgta atggtaagtt atccaataca agttagtgaa gttattttta
     7021 atttacttgg aagtcacgat actacaagaa tattgactta tgctaatgga aatgttgata
     7081 gatttaaact tgcatatatg tttatgtttg tacaaacggg atgtccttgt atatactatg
     7141 gtgatgaaat tggaatggaa ggagaattaa gttcaatatc tgaaggtcaa agaaaatgta
     7201 tggaatggaa tgaagaaaaa tggaatagag agatatttaa ttttatgaag aagattatta
     7261 ggcttcgtaa agaaaataaa gagtttaggt ctatatcaaa tgaatgggtg ttagcagata
     7321 aggaaaatgg aacaattata ttgaaaaaag aatccatttc tataataata aataattcat
     7381 ctaaagatat aattttgacg ttaccagatt atttagcaaa taaaaaagta aaagatttat
     7441 atgaagaaga aatagttgat ttaaaagaag aactaatatt agagaaatac aagttcatta
     7501 ttttaaaata atacaaaaaa gtatttattt tagatttcat gttaaattat tatacagttt
     7561 aaacaaatct ttatgaacga taaaaaaata tggtttacaa gtgagtgaat tgaaagtaga
     7621 ttttaaatta aagactgagt tagagtagat aattttataa ataatatctt aaatacaaga
     7681 tagaaaataa attgattatt agtctaaaaa attcttgtag aaagaggaaa taagaatgat
     7741 tgaagcattt atatttgatt tagatggtgt aattacagac actgcatatt atcactacat
     7801 ggcttggaaa aagctagcac ataagattgg tataaatata gatacaaaat ttaatgaatc
     7861 tttaaaaggc ataagtagga tggaatcact agatagaata ttagaatttg gaaacaaaaa
     7921 agatttattt tcaaaagaag aaaaattgaa aatggcagaa gaaaaaaata attattatat
     7981 atcatcaata aatgatataa cttcgagtga tattttgcca gggatagaaa gcttgttaat
     8041 tgatgtaaaa tctaataata taaagatagg cctatcttct gcaagtaaga atgcaataaa
     8101 tgtattaaat catttgggta taagtgacaa atttgatttt atagctaatt cagcaaaatg
     8161 taaaaacaat aaaccacatc cagatatatt tcttatgtca gcgaaaggac ttaatgtaaa
     8221 tccacaaaat tgtataggga tagaggatgc aagtgcaggt atagacgcta taaactcagc
     8281 taatatgttt tccgttggtg ttggaaacta tgaaaactta aaaaaagcaa atttagttgt
     8341 agattcaaca aatcaattaa agtttgaata tatacaagaa aagtataatg agtatatagt
     8401 taggagaatt atatgaaatg tttatttagt aatatagatg agtggaagat tatacaagat
     8461 aagattgaaa tcaaagaaaa tagattagca gagtccataa tgagtatagg caatggatat
     8521 atgggtatga gaggaaacta tgaggaaaat tatagtggag atacacacaa ggggtcttat
     8581 atagctggag tatggtttcc agataaaact cgtgttggtt ggtggaaaaa tgggtatcca
     8641 gaatactttg gtaaaattcc taattcagtc aactatatag gtgtaagaat tttcatagat
     8701 gatgtagaat tagatttagc aaaatgtaaa gtagaagatt ttcatagaga attagatatg
     8761 aaaaatggta ttttaaatcg taggtttatt gtaaatataa atgataaaaa atttgaagtt
     8821 agggttacta ggtttttaag tatatctgtt aaggaacttg cagtaattaa atacagtata
     8881 aaatcattaa attctaatac taaaataaaa tttgtaccat atttagattc aaatataaaa
     8941 aatgaagatt caaactatga ggaaaagttt tggaatggaa tttcttcaag ttctttagaa
     9001 tataaaggga gtattctttc acgaacaaag gagaatgcat ttaatacacc agtattcact
     9061 gtgggttcaa tgatgtatat taagacaaat ggtgatgtag aagctatatc tcatgagagt
     9121 gattataatt actgtgaaaa ttcgataaca atggatgctt taaaaaatga ggttgcaagt
     9181 atagaaaaat atgtatctat aacttcaact agagattata aggaaagtga gctattaaga
     9241 aaatgtgaag acattttaaa tagagaacta tctaaaagct atgatgatat attaaatgaa
     9301 catacaaagt tatggaataa aagatgggaa agtgcagatg taaaaataaa tggagataaa
     9361 agtagccaac aaggtataag atataattta ttccaactat tttcaacata ttatggagaa
     9421 gattcgcgat taaatatagg tccaaaagga tttactggtg aaaagtatgg aggagctact
     9481 tattgggata cagaagcgtt ttgtgttcca gtatatctag gtgtagcaga taaggaggta
     9541 actagaaatc ttttagaata taggtacaat cagttagaac aggcaaagga taatgcaaaa
     9601 aaactagggc ttaaaggtgc tttatatcca atggttacat ttgatggagt agaatgtcat
     9661 aatgaatggg agataacatt tgaggaaatc caccgaaatg gaagtatagt atatgcaata
     9721 tataactata caaattatac aggtgactat aaatatataa aagaaaaagg aatagatgta
     9781 ataatagaaa tagctagatt ttgggcaagt agagttcatt tgtctacaaa aaaagatttg
     9841 tatatgatac atggagtaac aggcccaaac gagtatgaaa acaatatcaa taataattgg
     9901 tatactaatt atatagctaa atggtgttta gattatgctg tagaaaatat cttacgatta
     9961 gagaaagagt ataatgaatg tattgacaga aataatgtga ttagggaaga aattgagttt
    10021 tggaaaataa tatcaaataa aatgtatctg ccatatgatg aggaactaca aataataatt
    10081 caaaatgata attttttaga taaagaattt gtagaagtaa aggatttacc aaaagaaaat
    10141 tttcctctta atcaaaattg gtcttgggat aggattctta ggtcgtgttt tataaaacaa
    10201 gcagatgtat tacagggaat ttactatttt ggaaatagat ttactaaaga agaaaaaaag
    10261 agaaattttg acttctatga aaaatatact gtccatgaat cttctttatc ttctagtatc
    10321 tattctatta ttgcctcaga aatagataac ttagaaaagg cttatgaatt atactcaaga
    10381 acagctaggc ttgatttaga taattacaat aatgatacaa atgatggact gcacataact
    10441 tcaatgtctg gttcatggct ttccattgta catggatttg caggcatgag aacatgggat
    10501 gaaactctat cttttgaccc taagttgcca aaagagtgga aaaattattc tttcaatata
    10561 aattatagag ataatattat taatgtagat gtatgtagtg atttaattaa aatagaaaat
    10621 ttaaaaggta aaccaataaa aatt
//
LOCUS       DAEORZ010000140        10591 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEORZ010000140 DAEORZ010000000
VERSION     DAEORZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.142"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            95..661
                     /locus_tag="KRH39_003107"
     CDS             95..661
                     /locus_tag="KRH39_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBG8512992.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
     gene            966..1427
                     /locus_tag="KRH39_003108"
     CDS             966..1427
                     /locus_tag="KRH39_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8512993.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            1495..3741
                     /locus_tag="KRH39_003109"
     CDS             1495..3741
                     /locus_tag="KRH39_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512994.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKVMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPEAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            3734..5566
                     /locus_tag="KRH39_003110"
     CDS             3734..5566
                     /locus_tag="KRH39_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8512995.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            6285..7562
                     /gene="hflX"
                     /locus_tag="KRH39_003111"
     CDS             6285..7562
                     /gene="hflX"
                     /locus_tag="KRH39_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG8512996.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            7795..8292
                     /locus_tag="KRH39_003112"
     CDS             7795..8292
                     /locus_tag="KRH39_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8512997.1"
                     /translation="MDAETLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIEIEQNEKDSRAI
                     CLVTKDRVEEYSKKVRGMQEKVLQLLFEDFTEKEAEQLFFGLMKLSIGIENVKNYVEN
                     NDVGE"
     gene            8341..8865
                     /locus_tag="KRH39_003113"
     CDS             8341..8865
                     /locus_tag="KRH39_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG8512998.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            9177..10541
                     /locus_tag="KRH39_003114"
     CDS             9177..10541
                     /locus_tag="KRH39_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBG8512999.1"
                     /translation="MDIKELVKMQRKYYNTDKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSASTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQKHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGVGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
BASE COUNT         4196 a         1069 c         1893 g         3433 t
ORIGIN      
        1 aatttaataa atataaatta aattatatgt gtatatattg tactttaata gtgatataat
       61 aataaaaaaa tttttaaaga gggtgatatt acatatgaga aaacttgaag agatattaga
      121 atataataaa tcgtttgtag agaacaaaga atatgaacaa tatgtgacat caaaacatcc
      181 aaataaaaaa atagtagtct tatcttgtat ggatacaaga ttaactgagc ttttgcctaa
      241 ggctatgaat ttgaaaaatg gagatgtgaa actaataaaa aatgcaggag ctactataat
      301 gcatcctttt ggaagtatta tgagaagtgt tttagttgca atttacgaat ttgatgtaga
      361 tgaggttatg gttgtagggc atcatggatg tggtatgtgt aatgttgata cggataagtt
      421 acttggtaaa attttagata gaggaatatc taatgatgta atacttacac ttcgtagtgc
      481 tggaattgat acaaaacaat ggttacatgg attcgattct gcagaagaat ctgtaaaaga
      541 tagtattgat ttaataaaag ggcatccttt aattccagat ggtattattg tacatggtct
      601 aattatatca cctgaaactg ggaagttaga cgttgttgta aatggatatg aagataagta
      661 gagtatcata atgttatata atcaagatac tgttgaatat gatgttgttt caaaatttat
      721 tttgggacaa cttttttatt tgaaaatata tgaaaaggtt tattttaagc tttgcaataa
      781 aaaatataag tagctgacta attaaaaatc ttactattat ttattaaaat tataactata
      841 tgatataaaa atatttaata aatgaaattt atatgaattt tttataaata tattgaaaaa
      901 atgcatgcag tatagtaaaa taatacttag ctgactaagt aatataaaaa ataaggaggg
      961 caagtatgaa tagcaattct gaagaaaaat taaatgatgt tttcttgcaa ttaatgcgaa
     1021 tatattttat aagaactcat ttttattttg ataaattagg cttatatcat ggtcaacccc
     1081 aaatactttt ttctttaaag atgaaagatg ggcaaagtca aaaagaatta gcagataaaa
     1141 ggaaagtaaa agcatctaca actacagtta tgataaagag gatggaaaaa gctggacttg
     1201 tggaaagaaa acaagatgaa aaggaccaaa gagtatctag gatttttttg acagacaaag
     1261 gtttagaaat atgtaataaa gttgaagaat ttaacaagga attggaaaga gagttttttt
     1321 taggatttag tgaagaagaa aaaatagtac ttagaagact tctaatgcaa gtacgagata
     1381 atttaaaagc aggatgtcat aagcatgaag atgaaaaata tttttaaagt ttaaaataat
     1441 gtttttcaat ttaatgttat tagactaatt aatattacag aagggaagaa atgtatgttt
     1501 aaattaatta aatatttaaa gaaatcggca ttatcaatag taataatagt ctgtctattg
     1561 gtaattcaag cggtatgtga cctttcttta ccagaataca catccaatat agtaaatgta
     1621 ggtatacaac aaggtggagt agaaaactct gttccatctg tgattagaga gagtgaatta
     1681 aataaaatta ctttatttat ggataaaagt agtaaggata aagtactaga taattatact
     1741 ttgctgaata aaaaggatta tgttaagtat aaagataagt acccaggtct aaaagatgaa
     1801 tctctttatg agttaaatac taaagacaaa gacactatag atgatttaaa tgttattttt
     1861 ggtaaagcta ttttaatagt atcagggctt gagggcgata gtaaagaggt aaaagttatg
     1921 aaagctcagt taatgagcaa acttccacca caagctactc aaagtggaga tgttgatata
     1981 tttaaattgt tatcagcaat gccaaaggaa cagttagata cattgactaa agagatgagc
     2041 aaaggctttg aaagtatgcc agagagtatg ataacacaaa gctctgtttc atatgtaagg
     2101 tcagaatatg agaaaatagg tattgataca gaaaaaactc aaaataatta tatattattt
     2161 actggtgcta aaatgcttgg tatagcaatg attagtatgg tagctacaat aacagtagga
     2221 tttttagcag caagagtagc cgcaagtgta ggtagaaatt taagaagtgg agtatttaga
     2281 aaagttatgt cattttcaaa tactgaaatg aatgaatttt ctacagcttc attaattact
     2341 cgtagtacaa atgatattca acagatacaa atgttgatgg ttatgttgct tagaatagta
     2401 ttctatgcac ctataatggc tataggtgga gtgataaagg ttttaaacac aaatacttca
     2461 atggcctgga ttatagcagt agcagtagtt gctatcttat cattaattgt agtgttattc
     2521 tcagtggtta tgcctaagtt taaactggtt caaaagcttg ttgataagtt aaacttagta
     2581 agccgtgaaa taattactgg tattccagtt atcagagctt ttagtactga aaaacatgaa
     2641 gaagaaagat ttgataaagc aaataaaaat ttaactaaaa caaatctttt tgtaaataga
     2701 gttatgacat gtatgatgcc tgctatgatg cttataatga accttatcac agttcttata
     2761 gtttggagag gtgcatacag tgttgattct ggagcaatgc aagttggaga tatgatggca
     2821 tttatacaat atacaatgca aataataatg tcattcttaa tgatatcaat ggtatctatt
     2881 atgcttccaa gagcatccgt ttcagctaca cgtatagatg aagtattagt gtcagatata
     2941 agcataaaag acccagaaaa atcagaggca caagagttta aggaagataa aaagggtctt
     3001 gttgaattta aaaatgtttc gttcatgtat ccagaagcag aagaacctat attaacaaat
     3061 atatctttta cagcaaagcc aggagaaaca actgccttta taggaagtac tggtagtggt
     3121 aagtcaactc ttataaattt aattcctcgt ttctttgatg ttactgaagg agaaatatta
     3181 cttggtggag ttaatataaa aaatgtttct caacatgatt taagggaaaa aattggatat
     3241 gtacctcaaa agggagtatt gttttctggt actatagaat ccaacttaaa atatggtaga
     3301 aaagacgcaa cagatgaaga aatgagaaca gcagctgaaa tagctcaagc tacagaattt
     3361 atagattcta aaccggaagc ttttaaaact gaaatttctc aaggtggaac aaatgtatct
     3421 ggaggtcaaa aacaaagact ttctattgca agagcaatag caaaaaatcc agaagtttat
     3481 atatttgatg atagtttttc agcacttgac ttaaaaactg atgcagctct tcgtaaggct
     3541 ttaaaatcaa aaactgctga aagtacagtt ctaatagttg cacaaagaat tagtactatc
     3601 ttaaatgcag aacagataat tgttttaaat gagggcgaaa tagttggaat aggaacacat
     3661 aaagagcttc taaaaaattg cgaagtttat gagcaaatag ctttatcaca actttcaaag
     3721 gaggagctag ctaatgagta gaatgggaag aggtcctatg ggaaaatcta tgggagcagg
     3781 acaaaaagca aatgacttta aagggactat gcgaaaacta attgcatatt tatctaaatt
     3841 taaaatatca ataattctag taattgtatt tgctattggt agtgcgtcgt tttctatagt
     3901 aggtccaaaa attttaggaa aggcaactac aaagatattt gaagggctag tgagtaaagt
     3961 ttctggagga aacgtaggta tagattttaa tgcaattgga aaaatcttaa cctttttact
     4021 tttcctttat ttgataagtg cactgttctc ttttatacaa ggatttataa tgagtggaat
     4081 ttctcaaaaa gtatcctata atttaagaaa agagatttca gcaaaattgg atagattgcc
     4141 aatgaagtat tttgatacta agacgcatgg tgaaatatta tcgagaatta cgaatgatat
     4201 agatacttta aatcaaagtt taaatcaaag tatgacacag cttataacat cagtaacaac
     4261 tatgataggt gttttgataa tgatgttatc tataagtgga attatgacat tagtggcagt
     4321 tttaatatta ccaatatcaa tgtttgtaat ttcaagaata ataaagaaat cacaaaaata
     4381 ctttagatat caacaagaat atttaggaaa tgtaaatggt caagttgagg aaacttacag
     4441 tggccaaaca atagtaaaag catttaatcg tgaggatgaa gtaattgaag agtttgataa
     4501 actaaatgat agcctgtata attcagcgtg gaaatctcaa tttttatcag gtataatgca
     4561 accacttatg atgtttattg gaaacttggg atatgtaatg gtatcaatct taggaggttg
     4621 gcttgcaatt aagaaaacaa ttgaggttgg tgacatacag tcatttatac aatatgtaag
     4681 aaactttaca cagccaatga ctcagattgc acaggttgcc aatttattgc aatcaacagc
     4741 agcagcttct gaaagagtat ttgagttttt agaagaggaa gaagaagtcc aaattgttga
     4801 aaatgcagtt tcaatagatg gtttagaagg taaaattgat tttgaaaatg tcaactttgg
     4861 atataaccca aacaagacta taataaatga tttctcagta aatgttaaac ctggtcaaaa
     4921 agttgctatt gttggaccta caggagcagg taaaactaca atagttaaac tgttaatgag
     4981 attttatgat gttaatagcg gttcaatact gattgatggt cataatatta aagattttaa
     5041 tcgtagtgag cttagagaaa tgtttggtat ggttcttcaa gatacatggt tatttagtgg
     5101 ttcaattatg gaaaatataa gatatggtaa gttgaatgct acagatgaag aagttataga
     5161 ggctgctaaa tctgcacatg ttcatagatt tattaagaca ttaccagatg gatataagat
     5221 gaaattaaat gaagaagcaa gtaatgtttc tcaaggtcaa aaacaattat tgactatagc
     5281 tcgtgctatt ttagctgatc ctaaaatttt aatattagat gaagctacaa gttctgtcga
     5341 tactagaaca gaagtattga ttcaaaaagc tatggataat cttatggaag gtagaacaag
     5401 ttttgttata gcccacagat tatcaactat acgtgatgct gatatgattc ttgtaatgaa
     5461 tgaaggagat atagttgaac aaggaaatca tgaggaactt ctaaagaaag gcggattcta
     5521 tgccaatctt tacaattctc aatttgaaga agatgaagca atgtaacaaa aaactatctt
     5581 agcatagaca tgtaattaat aagataaaat aaatgagggt gccttaaaat gaacttttta
     5641 attcataagg cactcttttg tgtatgaaat aaagtgtatt tttatcattt taacaatgaa
     5701 aaagaggatt ctctatattt tgagatagcc acataatttt atgtctttac aaagtgttta
     5761 ttttattata atatgtagta aggaaaatgt tggaggtgat tatcaaaatg tatatttaat
     5821 tttattcttt aaattttatt ataaatagaa tttaatttta atagttgatg attatattga
     5881 agtatatgtt tttattactt ttattaatca tgtaaataac tacttaaatt taagaatagg
     5941 gttaaatata aaatgataat tacatataag ttaattaaac taatgtattt ttaatatata
     6001 tttagaattt taatccttct tatgatataa gtagcttgtt ttgtattact tagatttgta
     6061 aggagttttt ttattgaaaa aactgcataa tatatttggg aggaggtgta agaaagtgat
     6121 tttttatata agattgagaa aataatgcac gattatggtt attataatag taaataagaa
     6181 gtgtcattta aggataagat aaaaagcaaa attcttgatg aatttaatta tagagccata
     6241 atctcgtgct atagattaaa taaaaaatag cagataagga gaatatggat atggcgaaaa
     6301 aaggaataac agtaggatta aacataaata ataaatctga ggactttaat gaactaatga
     6361 ttgaacttga aaacttatgt tcagcatgtg atattgatgt tgtagggagt ataactcaaa
     6421 atgcaaagca ggttaataga gcattttaca ttgggacagg taaagttgag gaaatattaa
     6481 acttaataaa gaaagaaaat atagagatag ttatatttta caatgaactt tctacatcac
     6541 aactaaaaaa tcttgaagaa aagcttaatt gtgaaattat agatagaacg gcattgatac
     6601 ttgatatatt tgcacaaagg gcaaaaacta gagaagctaa acttcaagta gaagtagcaa
     6661 gtttaaaata tatgttacca agacttatag gttcaaatga aaatttagga cgtcaaagtg
     6721 gtggagttgg tactaaaaat agaggttctg gtgagaaaaa attagaactt gaccgtagaa
     6781 gaatagaaga aaagataaca tctttaaata gggagttgga tgatttaaaa ttccaaagag
     6841 aaactcaaag aagtatgaga agaaaatcaa atcttccaaa tgtagctcta gtcgggtata
     6901 caaatgctgg taaatcttca ataatgaata aactagtaga tatatttaaa aactctgaag
     6961 aaaagaaagt gtttgagaaa aacatgttat ttgctacact tgaaacttct gtcagaaata
     7021 tagttcttgc aaataacaaa gaatttttat tatctgatac tgttggtttt gtaagtaatc
     7081 taccacatga tttagtaaaa gcatttaggt caacattaga agaagcatgt gaggcagacg
     7141 tactgttaca tgttattgat atttcaaatc caagctataa aagtcatatt aaagtgacag
     7201 aagatacttt aaaacaaata ggtgcagatg gtatacctat gatacatgtg tataataaaa
     7261 ttgatttaat agatgtagaa gtattggata gaattttaga tagtatcgat aaagaaggta
     7321 tatttgtttc agttaaaaaa gatataaata tagataaaat gataaaatgt atttgtgata
     7381 gcatatttaa agattatgta agatgtaagt tcttaattcc atatgataaa ggtcatgtag
     7441 tttcttattt taacgaaaat acgagcatta taaatacaga atacagagaa gatggagcta
     7501 ttttagatgt agaatgtagc aatatagaat ataataagta taaaaagtat gctttagagt
     7561 aaattttgaa aaacttatta tagcctaaaa tatatttaat ttataattta ttataaattg
     7621 attgtaaata tgattttatg aaacggtata gccataatgc tatgccgttt tgccatttat
     7681 aataaaaata aatttattga cacaatagta actaattact ataatataaa gtaactagtt
     7741 actatttaaa attatcaaaa aattaaaatc taagataaag agaagtgtat aataatggat
     7801 gcagaaactt taatgaaaaa atatatggta gatgatgata gtagaaaaca agctatattt
     7861 gcaagtatat ttgtaatgca aaataaactt caaactacat gtgataaatt agacccagaa
     7921 ttaacaatga aacaatggtt acttttagct attgcggact ctgtagatga aactcttaca
     7981 ttaaccaaac ttggagaatt aatggggtgt tcaagacaaa atgtaaaaaa actagctatt
     8041 gctttagaaa agaaaggttt tatagaaatt gagcaaaatg aaaaagattc aagagctatc
     8101 tgtcttgtga caaaagatag agtggaagag tattctaaaa aagtaagagg tatgcaggaa
     8161 aaagtattac agcttttatt tgaagatttt acagagaaag aagcggaaca gttatttttt
     8221 ggacttatga aattaagtat aggaattgaa aatgtgaaaa attatgtaga aaataatgac
     8281 gttggagaat aagcaagtat caaatcaact ttgtatgaca tcagagaggg gagaatttat
     8341 atgaaaagtg tagtagtgat ttataagagt aagtatggtt caacaaaaaa atatgctaaa
     8401 tggctttcag aattattgtc ttgtgatatt tttgaaacta agaatattaa tatagaaaag
     8461 gttatgaagt atgatacgat tattttagga ggaggtcttt atgcttgtaa tatagcggga
     8521 atttctttct taaaaaaaaa ttataaagaa ttgcagaaaa aaaatttagt agtatttggc
     8581 gtaggagctg ctccatatga tgatgaagta ataaagaaat taagagaagt aaattttaag
     8641 gatgaattaa acaaaattcc ttgcttttat tgtcgtgggg catgggatga gacaaaaatg
     8701 tcttttatag attatactct ttgtagtttt tttaaaaaat ctatagctaa aaaagaacgt
     8761 caaggtctta aaccagcaga agcaccttta ttgtctggag aagcatttga ttggtgtaat
     8821 aaagaaaatt taactccaat acttgattat ataagagaac tttaagaggt atatatttat
     8881 aattatttat tatctaaagt tagatttcat agaaacttac tagacattat ttataaaata
     8941 atattattaa agacggataa tttaattgat tcaataaggt tataatttta aaaaatgttt
     9001 attttaaaat aaattatttt ataagcttac ttatacttga taaggggctt tttttattgg
     9061 aagatttatg ttatattgag atgaatttat tatgatataa aaaataatgt taggataaat
     9121 ttaaatcttt ttctaaaaat gtattagtat aattcttaaa gaaaggattt ttaataatgg
     9181 atataaaaga gttagttaaa atgcaaagaa aatattataa tacagataaa acaagagata
     9241 tatcgtttcg tatagaacag ttgaagaaac ttaagttagt tgtatcacaa aatgaagaaa
     9301 aaatactttt agcactaaag aaagatttaa ataaatcgga ttttgaaggc tttatgacag
     9361 aagtaggcat gttttatagt gaattaaact ttgctataaa gaatataaga aaatggagca
     9421 aaataaaaag agttaaatca agtatggtta attttccatc tatatccaaa gttgttccac
     9481 aaccttatgg tgttactctc ataatgtcac cttggaatta tccatttcaa ttagcactta
     9541 taccattagt ttggtcatta gcagcaggga attgtgttat attaaaacct tctgaatatt
     9601 ctgcgtcaac atctagtgta gttaaagaca tagttgaaga tactttttca aaagaatatg
     9661 ttgcagttgt tcaaggaagt caagaagaaa gtgaaaagtt actattggaa agatttgatt
     9721 atatattttt tacaggaagt actaatgttg gtaaaataat catgaaatca gcatcagagc
     9781 atttaactcc tattacttta gaacttggag gaaaaagtcc ttgtatcatt ttaaaagatg
     9841 ctaatataga ccttactgcc aaaagactta cttggggaaa actaataaat gcaggacaaa
     9901 cctgcgtggc tccagattat gttttagtgc atgaagatag aaaaaatgag ttaatagaga
     9961 aaataaaata ttatactaat aagtattttg gagataatcc atgtaataat gagcaatttc
    10021 ctaaaatcat aaatcagaaa cattttaatc ggatattatc tcttattgat aaagataaga
    10081 tagtctatgg tggaaattat aataaggaga cattaaagat agagccaact atagtagaca
    10141 atgttaattg ggatgataat ataatgaagg aagaaatatt tgggcctata tttcccattt
    10201 taacatataa agatttggat gaagtaatac aaaaaattat acaaatgcca aatccattgg
    10261 cactttatat ttttacaaaa aataagtacc tagaaaataa acttttagaa atgattccag
    10321 caggaggatg ttgtatcaat gatacagtta ctcatatagc tacgaattac ttaccttttg
    10381 gtggtgttgg tgaaagtggt atgggctcat atcatggaaa agctggattc gatacattta
    10441 ctcactataa gagtgtgtta aaaaaattaa atttggatgt ccctattaga tatgctccat
    10501 acgataataa attaattaag gttcttaaaa agataatgta ggttatattg tagaataata
    10561 aatataaata aaattgagta tttgtattct g
//
LOCUS       DAEORZ010000141        10588 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEORZ010000141 DAEORZ010000000
VERSION     DAEORZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10588
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.143"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..383
                     /locus_tag="KRH39_003115"
     CDS             <1..383
                     /locus_tag="KRH39_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG8513000.1"
                     /translation="HVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVKINLNIK
                     GYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFGNMKSIY
                     LKKTNGNLIELSNHRITESKPWKI"
     gene            371..1309
                     /locus_tag="KRH39_003116"
     CDS             371..1309
                     /locus_tag="KRH39_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG8513001.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVTLEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSILTVFLIIFILKQNNLI"
     gene            complement(1693..1917)
                     /gene="secG"
                     /locus_tag="KRH39_003117"
     CDS             complement(1693..1917)
                     /gene="secG"
                     /locus_tag="KRH39_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBG8513002.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2522..3814)
                     /gene="eno"
                     /locus_tag="KRH39_003118"
     CDS             complement(2522..3814)
                     /gene="eno"
                     /locus_tag="KRH39_003118"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBG8513003.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
     gene            complement(4402..5934)
                     /locus_tag="KRH39_003119"
     CDS             complement(4402..5934)
                     /locus_tag="KRH39_003119"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBG8513004.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
     gene            complement(5943..6686)
                     /locus_tag="KRH39_003120"
     CDS             complement(5943..6686)
                     /locus_tag="KRH39_003120"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBG8513005.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEIKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            complement(6712..7914)
                     /locus_tag="KRH39_003121"
     CDS             complement(6712..7914)
                     /locus_tag="KRH39_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBG8513006.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            complement(8136..9143)
                     /gene="gap"
                     /locus_tag="KRH39_003122"
     CDS             complement(8136..9143)
                     /gene="gap"
                     /locus_tag="KRH39_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG8513007.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            complement(9203..10225)
                     /locus_tag="KRH39_003123"
     CDS             complement(9203..10225)
                     /locus_tag="KRH39_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG8513008.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
BASE COUNT         3252 a         1931 c         1196 g         4209 t
ORIGIN      
        1 agcatgtggt cattactaat aataatatag aaaattatat caatttttat aaaaaatttg
       61 gtttttcatc taaagaaatg agcaaaaatt ataaacttaa tgctagaaat gttaagataa
      121 atcttaacat aaaaggttat aaatttttac cttatgttaa aaattcaaaa gcaagtagag
      181 ataacttatg ttttaaaata aacacaaata ttgatgagcc aaaaaaatac ttagaacaaa
      241 atgagattca aattaagcga ggaattgtag aatgtacagg tgtctttggt aatatgaaat
      301 ctatttattt gaaaaaaact aatggaaatt taattgaatt atcaaatcat agaattacgg
      361 agagtaaacc ttggaaaatt taatcctatc aataaatgtt gttcttcctt tgtttctcac
      421 aatgtcactt ggatatgttc taaaaaaatt aggtctattt gatactcata ctttaaataa
      481 aatgaataat gtctgcttca agtctttttt gccattatta ttatttttta atatatacaa
      541 tacagattta gatggttctc ttaattttaa gcttatggct gtagctgtta taattatttt
      601 ttcaatcttc ttaatcttag tctttattat tcctaaaatt gaaaaagata ataagacaag
      661 gggtgcatta atacaaggtg tatttcgtag caatttcgtt atatttggta ttcctgtgac
      721 aatttcacta tttggcgata agagtattgg agtcacatct ttattaattg cagttattgt
      781 acctatgttt aatgtattat ctgttgtaac tcttgaaatt tttagaggtg gcaaagtaaa
      841 tataaaacat atgattaaaa gcgtcttaac aaatccactc ataatcgcct cttttatagg
      901 attgacaatg ctaattttaa atattaatat acctacattt ttacacaaaa ctattggtga
      961 tatttctaaa atagctactc ctttatcact tataattcta ggcggttctt ttagtttttc
     1021 tgctataaag gggcatataa aacccattat attgggcgta tgtggaaaac ttatacttgt
     1081 acctgctata tttttaccaa ttagcatttt tcttggattt agaaatgtag aattagcttc
     1141 acttatgata atgttatctg ctccaactgc agtttcttca tttacaatgg cacaacagat
     1201 ggattctgac agtgaattag ctggacaaat tgtagtattt acttctggat tttctatact
     1261 tacagtattt ttaataatat ttattttaaa gcagaataat cttatttaat gatatttgat
     1321 aacacaaatt ggtattatga gaaacatata taaaattgat ttaactgcgt aaaaacatat
     1381 tattatacta tttttactat ataatataac tttgtgtata ttatctcatt aaacatggtg
     1441 aaagtagtgt tgctatatat tttttagttt gaaaaaaata tataaataca aacaatatat
     1501 aaatacaaat aataaacgcc aacaaatagt caatctataa aataaaaact ggactaatca
     1561 tataaaatca ttagtccagc ttttatttta cgtacttttt atgttgatgt acttttatta
     1621 aaaactaaat ttaattttta aatgtctagc aaatgcacta attaaataat ggccagttat
     1681 tcaacaatat cactacttct taactataag taatgccaat gcatttataa aaaataatac
     1741 tgctgttatc tttgtaactc tagcaagaaa tgcttgtttt ccttttggtt taaagtaagc
     1801 ttgacttcct tctccaccaa attctgaagg taaagcattc ttagaatctt gaggcattat
     1861 gctaattact agtaaaatcc ctagtactac ttgcacaccc attaaaatgt tgctcattct
     1921 tgcccctcct atctgtttat tacttaaatt tcattactct ttagtgtatc acaataatat
     1981 agccatttca agactattat gctataaata acatacaaaa aaatatattg ttactataat
     2041 atattactat attctcaata taaaaatgaa taaaacttag aagagaaaac tatttaaatt
     2101 aatacataaa ttaatacata aattaacact taaaataatc tactctcatt gtatatatta
     2161 taaatagcca ccaaaataaa acaatcattt taaaatattg atataacttt taatttcaac
     2221 ataatttaaa ataaataatc cacatttagc aataatactt taatgacaat tatgattaaa
     2281 tcaaaatatg tcttcacttt tattttttaa taaaaacctc ctatgcaatt agataaaaat
     2341 gagatagaaa catttattca tattttttct gaatctatga ataaaattaa gtaataaaat
     2401 ataaaaaagg ataagtgcct tgaaaaatca attaacactt atctctttta tatcctataa
     2461 gatgagctta aatatcttat attctctctt cattaatctt ttttagtaac cattttaata
     2521 actatttttt taagttatag aaagatttta atccacaata tctagcttgc tctccaacca
     2581 tttcttctat tcttaataat tggttgtact tagcaactct atcagttctt gatggagcac
     2641 cagtttttat ttgaccagca tttactgcaa cagctaaatc tgctatagta gaatcttcag
     2701 tttctcctga tctgtgagat ataacagcag tatatcctgc tctcttagcc atttctatag
     2761 catctaaagt ttcagttatt gtacctattt gattaacttt aactaatata gagttagcta
     2821 ctccattttc aattcctttt tctaatctct ctgtgttagt aacaaataag tcgtcaccaa
     2881 ctaattgtaa tttattacct aatttttcag ttaataattt ccatccatcc caatcttctt
     2941 cgtctaaacc atcttctata gttattattg ggaagttgtt tgaccaatct tcatataaag
     3001 caaccatttc agcagcagtt aactcttttc cttctcctgc taaaacgtat tttttagttt
     3061 ctttgttgta catttctgta gcagcaacgt caagacctaa cataacatct tcacctggct
     3121 tatatccagc ttttgttata gcttcaacta ttaattctaa agcttctctg tttgaaccta
     3181 agtttggagc aaatccacct tcgtcaccta caccacaagc taatcctttt tctcctaaaa
     3241 ctttctttaa tgaatggaat acttctgctc ccattcttaa accttctttg aaacaacaag
     3301 ctcctactgg taatatcata aattcttgaa catcaacgtt gttatcagca tgctctccac
     3361 cgtttaatat gttcatcatt ggaacaggta attgtttagc atttactcca cctaagtatt
     3421 ggaataatgg taaacctatt tcatcagcag ctgctctagc tactgccatt gaaacaccta
     3481 atatagcatt agctcctaat ttacctttgt ttggagttcc atctaattct atcatgattg
     3541 catctatagc tggttggtca aatgcatcca ttccttctat ttcaggtgct attatttcat
     3601 ttacgttagc aacagctgtt tctacacctt ttcctaagta tcttccttta tctccatctc
     3661 ttaattcaac agcttcaaaa gctcctgtag atgctcctga tggaactatt gctcttccca
     3721 ttgctccatc ttctaatact acttcaactt ctacagttgg atttcctctt gagtctaata
     3781 cttctctagc atatactaat tcaataactg acatttgtat atctcccttc gattttctat
     3841 tagttaaatt ttcatttact gtaaacatcc gtctactatt ttaatctact ttctatatta
     3901 tagtacataa agttgctttt gaaaattagg tacaaattta tttgatgggt aaatttgtaa
     3961 aagttcctat ttttatttta ttttttataa tattttaatt tatataatat aaaaaaactt
     4021 gtctaaaatc caaaaccaat cttaaataca caaatattat aactagattt aaaacattaa
     4081 acataaaatg tacaactaat gattctaaaa tttttgctta tataatatat attaattatt
     4141 attatttata ttgttattgt ctatcttgtt attatttata tcattattta cattattatc
     4201 tatattgtaa ttttttattt tataaattgt ctatcttgta aattttataa aatatatctt
     4261 catctacacc ttacaatatc cagaacttac aaaatttaaa tcttatgtca taaagtctaa
     4321 actttacaaa ataaacctaa actgcattat ttttaaattt atattgtagt ttatatatta
     4381 ttatctactt atcagaatcg actattttga aataagtgaa tggccagtca tttcttctgg
     4441 cttttctaac ttcatcatat ctaaaacagt cggtgctata tcagataatc ttcctccatc
     4501 caataatttt gcactttcat attcttgacc aactacaata aatggtactg gattaataga
     4561 gtgagccgtt acagtttttc ctgtttctgg gtctaacata tattcagcat ttccgtggtc
     4621 agcagttatt attgcacttc cacctaactc tactatttta tctatcaatc ttcctacaca
     4681 agtatctaca gtttcaacag cctttatagc cgcttctata cttcctgtat gacctaccat
     4741 atcaggattt gcaaagttta atactataaa gtcaaactta tcttctccta atttatctaa
     4801 agctttatct gtcaattcat atgctgacat ttctggtttt aaatcataag ttgcaacctt
     4861 tggagaaggt atcaatagtc tttcttcacc tttatttggc tcttctactc caccattgaa
     4921 gaagaaagtt acatgtgcat acttttcagt ttcagcagct ctaagttgag tttttccatt
     4981 tttagctaga tactcaccta gagtgttggc taaagattga ggtccaaatg ctatatgcac
     5041 attctctata gttatatcgt actctgttaa gcatacaaag aagtttttaa tatcttctct
     5101 tttaaatcca tcaaactctt cacaaactaa agctctagtt atttgtctag ctctatctgg
     5161 tctaaagttg aagaatataa ttgaatcatt ttcctttata cttccaactg gtttatcatc
     5221 tttcataata actgttggca ttataaactc atcatttttt ccatcatcat aagagttttg
     5281 aatagcttcc tcaatagaat ttgctttttc accaatacct ctaaccatgg cattgtatgc
     5341 aagttctact ctttcccatc tcttatctct atccatagcg tagtatctac cagaaactgt
     5401 agcaaattca cctactccaa tttcatccat actagcttgt acttctttgg catattctaa
     5461 tgcactttga gggtcagtat ctctaccatc agtaaatgcg tgaacataca ccttttgtac
     5521 gcctttatct tttgccattt tgattatagc cttaagatgg tctatatgtg aatgtactcc
     5581 tccatcagac aaaagtccca ttacatgaag agaattttct tttgcattat ccattgcttc
     5641 acataaaact ttattagtaa aaaagtcacc atcttttata gattttgtta ttctagttaa
     5701 atcttgatat actattctac ctgcacctat gttagtgtgt ccaacttctg aatttcccat
     5761 ttgaccatca ggtagaccaa catcaagacc actagcattt atcaatgtgt ttggatactc
     5821 tttttttatt ctatctaaat taggagtttt ggactcagca attgcatttc ccttaacatc
     5881 cttattgtat ccaaacccgt ccataataat taaagcaact ggttttttca tcatttcact
     5941 ccttagaaat taacaaggtc taggtaatca ttactagcta aactagctcc acctactaaa
     6001 gctccatcta tatcactttg acccattatt tcggctacat ttgaaggttt aacacttcca
     6061 ccgtattgta ttctaacttc attagctaat tctccatata atccttttat aacttctctt
     6121 atataagata taacgtcatt agcatcttca gcagttgcag ttttaccagt tccaatagcc
     6181 cagattggct catatgcaac aactactttt gctaaatcat ctttaagaac attttctaaa
     6241 gctttttcaa cttgaacttt acaaacatct ttagtttttc cagcttctct ttgttctaaa
     6301 gtttctccaa cacataatat tgggtctatt cctacttcta atgcttttaa aactttcttg
     6361 tttacagttt catcagtttc attgaagtat tgtcttcttt ctgaatgtcc tattacaaca
     6421 tagtccatat ctatttcttt taacataaga ggtgaaactt ctcctgtaaa tgctcctttt
     6481 tcttcaaagt gcatattttg agcacctatc tttatatttg tacccttagt agcttctttt
     6541 aaatccttta ataaagtaaa tggtgcacat ataacagctt ctactttatc agaatttact
     6601 ttatctttaa tttcatttac aaattctaaa gcttctttta ttgttttatg cattttccag
     6661 tttcctgcaa ttataggttt tctcatttta caatctcctc tatatattag tttatttatt
     6721 atctaatgct gcaatacctg gtaattcttt accttctaaa aattctagtg atgctcctcc
     6781 acctgttgat acgtgagtca tcttgtcacc aaatcctaat tgattaactg ctgctgcact
     6841 atctccaccg ccaattacag tagttgcatc agtcaactct gccatagctc tagctactga
     6901 taatgtacca tttgcaaagt tttcaaattc aaatactccc attggcccat tccatactac
     6961 agtttttgat tcttttatag tttttacaaa gtttgctata gtcttaggtc ccatatctag
     7021 tcccatgtaa tcttctttta cattagcatc ttctgttact attggagtag catctggtgc
     7081 aaagtggtct gccataacaa cgtctactgg taataatagg ttaacaccct tattcttagc
     7141 tttttccatc atttctttag catattcaac tttatctatt tcaagtaaag aagttcctac
     7201 ttcataaccc tgagctttta agaatgtata agccattcct ccacctatta ttagattatc
     7261 aactttttct aataattcgt ttataacagc taatttatca gaaactttag ctcctccaag
     7321 tatagctgta aatggtctta cagggtttgc aacagcttct cctaagaatt ttaattcttt
     7381 ttgaattaag tatccacaaa ctctctcttg taaaaactcg cctgcaccaa ctgttgagca
     7441 atgtgctcta tgagcagttc caaatgcatc atttacaaat atttcagcaa gtgatgctag
     7501 ttctttagag aaattctctt cgtttttagt ttcttctttt ctatatctag tattttctaa
     7561 taatactaca tctccgtttt ccattttttc agttgctttc tttgcatttt ctcctacaac
     7621 attatcgtct gctgcaaata caacttcttt tcctaacatc tcagataatc tctttgctac
     7681 tggtgctaaa gataattctg gtttagcttc tccttttggc tttcctaagt gagagcataa
     7741 tataactttt gcccctttag aaattaaata ttgaatagtt ggtaaagctc cgttcaatct
     7801 attttcatca gtaattacac catcttgtaa tggcacatta aagtcacatc ttactaatac
     7861 tttttttccg cacacatcaa tatcttctat agtttttttg ttaagcattg acataaatat
     7921 ctctcctttt aataaagtaa ttttttattg ccaagtataa tatagtacat acttttaccc
     7981 tattaaatat atcatattat catattttta tatatagtat gtaacattat ttaggtgtat
     8041 tttatatatt ttttcttaaa aaaagtcctg agtccgtagt attaaactac aaactcggac
     8101 tttgtcaatc tacttttcta ttttctcaaa actgactatt ttgctaattg tgcaaagtat
     8161 cctaaagttc ttattaattg agaagtataa gacatttcat tatcatacca agaaacaact
     8221 ttaactaatt gttttccatc tacttccata acttttgttt gagtagcgtc aaataatgaa
     8281 ccgtagctta ttccgattat gtcagaagat actaacattt cttcagtata tccgaaagat
     8341 tcgtttgaag cttctttcat agctgcattt atttcttcaa ctgtaacatt ttttcctaat
     8401 gtacaaacta attcagttat tgaaccagtt acaactggta ctctttgtgc tgccccatct
     8461 aattttcctt ttaatgaagg tattactaag cctattgctt ttgcagctcc tgtagtgttt
     8521 ggaactatgt ttccagcagc agctctagct cttcttaaat caccttttgg atgaggacca
     8581 tctaaagtat tttggtcatt agtgtatgca tgtatagtag tcattaatcc tttttctata
     8641 ccgtatttat catttaatac tttagccatt ggagctaaac agttagtagt acaagaagct
     8701 cctgatataa ctgtttcaga accatctaat atgtcactgt ttgtattgaa tactattgtt
     8761 tttaaatctc cagtagctgg tgcagatata acaactttct tagcccctgc ttgtatatga
     8821 gcttcagctt tatctttaga tgtaaagaat cctgtacatt ctaatactat atctactcct
     8881 aattcagccc atggtaggtc agcaggattt ctttctgctg ttacttttat ttcttttccg
     8941 tttactacaa aagcaccttc tttaacttct atttctccgt taaatcttcc ttgagctgta
     9001 tcatatttaa ataaatgagc taacatttta gcgtctgtta agtcgtttat tgcaacaact
     9061 tcgaacttat cttgttgctc catcattttt cttaatgcta atcttcctat tcttccaaat
     9121 ccatttatag ctactttaac cattttcgct acctcctata aagtaaatgt atgttttcta
     9181 gctctgtgct aggtcttgac ttttataata atgacaatat cttataagca ctttcttcat
     9241 ctgttacaag aaccatgttt ttatgcatat ttgatatagc taataaggct tctacttttt
     9301 cagtccctgc aaagattgca atagtttctt ttacttgttt aactgtttct aaatcaattc
     9361 caacagtgtt cagtttgtaa actatttctc catctttgtt gaaataatat ccaaatgctt
     9421 ctcccactgc atttttagat aatatctcat caaccttgtc ttctggaagt tttcttctct
     9481 ttgccatgac atctgctcta cctatactaa aaactaaaat gtctgttctt ttaatatact
     9541 ccaaagttgt cttaatctct ggcacttggc taagagtttt cattgcactt tcatccaact
     9601 catctggtat atgtaaaagt ttgcaattag aatgtaattt cttacttatt gaagcaacta
     9661 tattgttaga ttgcatctct acatcagttc ccatacttcc tctagctgga actattgtag
     9721 agtttggata tctcttatca gttttaataa cgtttgaaaa ctccaacata gtgcttcctc
     9781 ctgttattga aacgatatct ccatctttta aaatgcctaa gaaatattcg catccatatc
     9841 tagttacatc ttttagtaaa cttccatttt catcacagct tccaggaact aatattaccc
     9901 tttttattcc aagcttttct ctgactctat cttgtaactt agacaatccc atgatgtcac
     9961 tcatgatgtc cttaagttta tctaacaaat cagaaccttc tttagtgata gtcatcccag
    10021 atacagctac gtctataagt ccttgttctt ttaaaaactc agtttctgac ctgattattc
    10081 tttcactaat ctctaataca tttgataatg ttctcctacc tattggttca cttaaagata
    10141 tttgtcttaa aatagagtat cttctttcca taagttcaat tgcctgtgga atcaatttct
    10201 tttgaatttc taataagttc ttcataacat actccttatt gctttccttt cgggactttt
    10261 tttgtcccta gtgtttattt aagtcccaca agatattttt attttatagg atttgcgtat
    10321 agatttcaat agtttttcaa aaactttttt atttttttat tttataaaat atttctccac
    10381 cattttcaaa tcacagtatt ttttcttttt atataaggct ttttatatat atttttttat
    10441 attttcattc taatatttat tactataaat aattttattt ttagttactt ttatttctag
    10501 ttatttttat ctacaactaa gtttactttt atctagttaa gatttttatt tataccctac
    10561 gtgttttcct tttgtgatta ttaaattt
//
LOCUS       DAEORZ010000142        10128 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEORZ010000142 DAEORZ010000000
VERSION     DAEORZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10128
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.145"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            38..1678
                     /locus_tag="KRH39_003124"
     CDS             38..1678
                     /locus_tag="KRH39_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG8513009.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            2036..3358
                     /locus_tag="KRH39_003125"
     CDS             2036..3358
                     /locus_tag="KRH39_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513010.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIAIETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            3555..4562
                     /locus_tag="KRH39_003126"
     CDS             3555..4562
                     /locus_tag="KRH39_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG8513011.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            4600..6027
                     /locus_tag="KRH39_003127"
     CDS             4600..6027
                     /locus_tag="KRH39_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBG8513012.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKN
                     KKIIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SVGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQMYMGVEE"
     gene            6171..7436
                     /locus_tag="KRH39_003128"
     CDS             6171..7436
                     /locus_tag="KRH39_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8513013.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            7478..9076
                     /locus_tag="KRH39_003129"
     CDS             7478..9076
                     /locus_tag="KRH39_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG8513014.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFNRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNVDDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            9111..>10128
                     /locus_tag="KRH39_003130"
     CDS             9111..>10128
                     /locus_tag="KRH39_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG8513015.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGST"
BASE COUNT         3682 a         1132 c         2011 g         3303 t
ORIGIN      
        1 aatatgaata taaataagtc ttgaaaggag aaaaagcatg tttgatgtta aaattgtcaa
       61 tggaataatc gttgatggta caggaaattc aagatataaa agtgatgtag ggattgtagg
      121 agacaaaatc atggctattg gagatttaag tcaaaaagag gctaaagaga ctatagacgc
      181 aactggaaaa tttgtatcac ctggttttat agatttccat actcattctg atttatctct
      241 tgtgtatgat aaatacacaa gaagccgtat ccatacaggt gtaacaacag atgttattgc
      301 aaactgtggt ataggagttg ctccgataag agaagagaag aagcaagagt taattgacta
      361 tcttggaact aggataattg gtacaattcc aacaaaatta gagcttcatt ggaatacaat
      421 gcaagaatat tttgattact taactgaaaa ttctccagca gtcaatgttg tagcttatgt
      481 agcacaagga cctgtacgta tcaatgaaat gggattttca aaggaaccag ctactttaga
      541 gcaacttaaa aatatgaagt tggaagttag aaaagctatg gaagctggat gtgtagctct
      601 ttcatcagga ttagtttatt taccaggagc atacacaaag aaagaagaaa tggctgaatt
      661 atgtaaggaa ttgataccat acaatggata ctatatatct catattagag atgaaggtga
      721 tgaagaaatg gaagctcttg atgaagcaat ttatatagct aagactgcag gagttcctct
      781 acatatttct catttaaaag ttatgggtca taagaatttt ggaactattg atgaggtatt
      841 taaaaagcta gatgaagctg aggcagatgg tttggaagta acttttgatt gttacccata
      901 tacagctgga atgacatctt tgggtgcact tttacctcct tgggcttttg aaggcggtgt
      961 agaaaatatg gtaaaacgat tagaagtaca agaaaataga gacagaatta taaaagagtt
     1021 ggaggaaggg attcctggat ggcaatgttt ctaccaatta gcaggtgggt ggaacggtgt
     1081 tgtactagcc tctgtaatga ctgaagcaaa taaatatgta gagggtaaaa ctttaatgga
     1141 ggtagccaaa atcaatggtg aaaatccatt tgatacgttc tttaggcttt tgattgaaga
     1201 aaaaagtaaa attcaagtag ttgttcatac acaaggtcaa gaggatacag ataaggttgt
     1261 ttgccatcca aagtcatgta taggttctga tagtatggac ctttctacag aaggtttact
     1321 ttctcttgga aagcctcatc ctcgtgcatt tggtactttt ggacgaattt tctcttacta
     1381 tgttagagaa aaaggtatgt tgacttttga ggaagcagtt aaaaagataa cttatctttc
     1441 agcaaaacgc ttaggcattt ataaagaaag gggtctactt aaagaaaatt attttgctga
     1501 cattgtggta tttgacccag atacaataga agataaagcg acttatagta atcctaaaca
     1561 gtatacagtg ggagtggaat atgtgttggt gaatggtaaa attgcattgg caggaggaaa
     1621 gcaaactgat gtatgtgctg gacgtgtaat taaaaatcct cttagcatag caaaatagaa
     1681 tacattaaga acttttataa aaatagtctt tctttgaata ttaaggaagg ctatttttgt
     1741 taaattaata aagattactc tacttgtgtt ttctaaagaa tttaacttta agtagaaaaa
     1801 acaatatatt aattgtaaaa tgtaattaat attaatgaat aagatttaaa tacaaaaatt
     1861 taaatacaaa aatgtttcaa cagatagtcg taattaaaaa acttaaagta actttttata
     1921 ttaggtataa ttgattattc aaaattctat gaatcaggag tcctgtgcct ttagtcatgg
     1981 taggtttaga aaatgtttta agagataata gtatcattag gaaaggagag acgaaatgaa
     2041 aatactttat gtaggaccag gtatggtaat cgataaaata ctagcagtaa gtaaaaagaa
     2101 ttttcctaat attgaagcag attttcttag atatgataga tatattgaag tacctgattt
     2161 attggaaaaa tatagaggta gagtggatgc agttcttttt acaggtaagt cgccatttaa
     2221 attttttgaa aaaagacaaa aagattttgt attgtctgat tatattccta gacatgaaac
     2281 aacattgtac agagtgttgc ttgaaattac atatttgctt aaattcgata ttagtagact
     2341 tagtatagat acttatgata aggctatgat gaagagattg tataaagaaa ttggagttga
     2401 atttaaggat gaacatatgt tttttgcaga gcaaaagtat ttagaagagg actatataaa
     2461 gtttgtgctt gaatttcata aatataatta tcaaaataat gatgtatgtt gttgtattac
     2521 aggtcttgaa gaggcctatc atataatgaa aaaagataat attccagtag ttttagcctt
     2581 gccttctgag gacgtaatgg tgcaaagtat aaaaaacatt cagatgcgtt atattgcaaa
     2641 gaaaaacaat gagaatcaga ttattgttct tgctattaaa ctgaacatgc cttcagaata
     2701 ttcactaatg aaggaagatg agtacgctta tctttcacaa aggattaaga ttcttgataa
     2761 actataccat ttcaatagcc gtatagatgg tgttttggta gagcagagta gaagtgaatt
     2821 tatgattttt acaactaaaa aaatacttga gttagaaaca aagaaataca gagatattta
     2881 tttgttggat atgattcgtg aagtttcagt tataaacact tatataggaa ttggttatgg
     2941 aaaaacagca aatgaagcta aatttaacgc ttatgagagt attaagatgg ctcaaagtca
     3001 aaaaaatgat gcagcatatg tagtttttga aaatggagaa attatgggac cactaaagcc
     3061 aaatcctact gtcaaagaga aaaatagttt tgatgaacgt ttttatcaaa ttgctataga
     3121 gacagggcta agtgctaata ctgtatataa agtattcaat aaaataataa atagtggaaa
     3181 aactgaattt acatcaaagg aattagctac tatatgtggt gtaagtatta gaactatgga
     3241 caggattata cttagattgt gtgattctgg ttattgtgag gtagtgagtg aacagcttat
     3301 gagcaagtat ggtagaccca gtagaatatt acgttttaaa ccttttctgt tgttttaaat
     3361 agatataagt caaaaattaa atttttatga taacattttc tttacaattt tttagatata
     3421 tgttaatatt tagaaaaatg catatgtatt cataaaataa agttaaaaca gctaaaaaat
     3481 cattaaaaag gaaatcgtat gcataaaatg agaaagttaa tttataactt aaataaatct
     3541 gggaggagat aattatggga aaatatcagg attcaaaaaa agttgtaaga aattattttg
     3601 aagcattaga aaatgctact ggggatgagg ttgaaaatgt cttaaagcaa catatgaaat
     3661 cagattataa ctggaaaggt gtgtatccat ttcgtgaaca agaaggcact gaaaatgtag
     3721 cagatgtatt ttggaagcct ttaaaaaaat cattgagtaa tatgcaaaga cgacaagata
     3781 ttttcattgc aggaacaaat gagattgaca atagtgaatg ggtaatgagt atggggcatt
     3841 ttatgggact ttttgataag gattggcttg gcattaaaca tactcgcaaa atgattagtt
     3901 taagatatgc agaatttaac tgtgttcaag atggtaaaat tgctaaaact ggattatttg
     3961 tagatataat tgggtttatg attcaagcag gagttaatcc attgccacca caaacaggtt
     4021 catattttgt atatccaggg ccaattgacc ataatggatt actttttgaa gatgcagatg
     4081 aagttgaagg agtaaaaact ttagcattag taaataaaat ggtagatgat ttatctgaat
     4141 taaatgaaag tggtgctatg ggatgcccac cagaaatact tgaaaaatct tgggctaaag
     4201 atatgatttg gtatggtcca ggtggaattg gtgcttctta tactatacct agatatcaag
     4261 agcaacacca gctaccattt agaaataatt taaaaggaaa gactttcaat gggcatgtat
     4321 gtagatttgc agaaggtaat tttgcttgtt tctttggatg gcctaatctg actaatgtac
     4381 catgtggagg ttttttagga ttaccaggtg gagaggttaa agcagatatg caagttgttg
     4441 atgtttatta tcgtaagggc gataaacttc aagaaaactg ggtgttaatt gatattcctt
     4501 attggttgaa acaacaaggg cttgatattc ttgagagaac taaaagtatt tttaatgctt
     4561 agttatgcat acgtattcta aaacaaaggg ggatataaag tggcatctac caacaaaaat
     4621 aatgcaacaa gtaaagaaga agaaaaagaa gtttctacaa gtgggcttga atcatgtacg
     4681 gttaaagaaa acaaagctaa aatgaatcaa actaatttta gtaatataaa tgaaaatcaa
     4741 acaaatgaga ataaaacgaa cacatataaa agaaatatag ataaaggaaa tatagataaa
     4801 gggaatatag ataaaacaaa cacaaataaa tccgacataa ataaagttaa catagataca
     4861 gtggatacag ataaattaaa tacaaatact cttactgaaa aaaataaaaa aattatattt
     4921 aagggggaag ataatatgtc atctacaaaa atttactatg gagattcatt agaagtaacg
     4981 cctgttggag taatggatta caatgatttt tcaaaacaga caaaaaggga acaggaaata
     5041 cctggatttg attctaagta tagagatttt gtagattata ttatgaagat tacacataat
     5101 atctgggaag aaaaaggtat aggcgttatt tatgatacat atcacaataa tgttacaatg
     5161 cattgtggct cttcaaattt agttggtatt aaagatgtta tttccaatac tcttcaaaca
     5221 ttacatgcgt ttcctgatag aagattaatt ggtcaaaatg taatatggtc taactttggt
     5281 gctgatggtt ttttatcttc tcaccgtgtt ttgtctacag caactaactt aggtgatagt
     5341 aattttggac cagcaacagg tagaaaaata aactttagaa ctgtaattga ttgtgccaca
     5401 actaacaata gaatccacga agaatggttg gttagagata atttatggat tgttactcaa
     5461 ttaggattaa accctcagga agtagcaaag agtatggcaa aggcaagtga atctaaagtg
     5521 cttagtttac aatcaactta tggtatctgt gaatctatgg atggtcaatt tatgccaact
     5581 aagtatcaag caaaagatga ttctgttggc gaaatgatgc ttgaaatgac aagccgtatt
     5641 tataattata aatatataaa tgaagttaaa aaatactatc atgataatgc agtagtacat
     5701 tttatatgtg ataaagattt aaatggttat gatgagattc aaggaatgat tgtaagttta
     5761 cttgcatcaa ttccaaatgg aagttatgaa gtggaaagag taacttgtaa tcaaagagaa
     5821 aaaaatgaag gttatgatgt atctgtaaga tggagattac gtggaataaa tgaaggtata
     5881 ggattttttg gacagccttc tggaaagcat atagaagtaa tgggaatcaa tcattatcat
     5941 atactacaag gtaaagtaaa ggaggagtgg ataacttttg atggtatgga tgtattaaaa
     6001 caaatgtata tgggagtaga agaataaagc tgtttagagt ataaatagtt ttgaaatata
     6061 tatagggaag tattgcttta taaaatttaa gtggggaatg tatatgaaaa atagatgttt
     6121 aaacttttag caaaattatg actaaattta aaagggggaa ttataaaaat atgaaaacaa
     6181 cggttgaaat agaaaatgat atcatattag gaaaagacta ttcaaaccta aaaagaggaa
     6241 ttgcattttt ttcaattgct ttaatatact ttttttattg ctacaatttt atggtgggta
     6301 cttttattaa accaacaatg atttatgctt tagcagatgg gggatttggt ttttcattaa
     6361 aacaaacaga agaaatattt gcagttatgt cttttggtac aattccaggt acatttattt
     6421 ttggggtgat ttctacaaaa attgggaaga agaggacttt aattagcgtt gctttactga
     6481 ttggattgac aacatttata ccaatgttat ctcctaccaa tattatgtta tggaaaatag
     6541 cacgtttgtg tacaggagta gtgttgggag gcgtatttgg aacagctatg cctttagttg
     6601 cagatatgtt tccaagtaaa tatcgtggta agttagctgc tatattaact gctttatttt
     6661 cattagcaat gatttttggt gggaaagtat atggcgtttt aggtgatgca aattggcaaa
     6721 tattgatgta tactgcgatt ataccaccta tagttggagc tatcttagca atcttctttg
     6781 tgccagatga tttggagtat acaaaggaat tagttaaaaa gggaaaagaa actggtgaaa
     6841 agattagcta tataagtatg tataagggaa aatacttatg gattggattg ggaacgattc
     6901 ttttatcagg tgcaaatttt acagcatatt cagcattttc aaataatgca actacatacc
     6961 tagtgacagg aataggaatg tcagcagcag tggctggttc tatatacagt ttacagggaa
     7021 ttggtcaatt agtaggatat ttattctggg gttcaattgc agataaattt ggaagaaaaa
     7081 taccttttat aggtatggca ttagcagcag tatttgtttt tatatataca aaattaggtg
     7141 gagataatac aacaacattc tatatgatat cagtattact aggtgttgct gttggatatt
     7201 ctggagcatg gggagcatac tacacagagt tgttcccaag taaatatcgt tcgttgtcat
     7261 ctggtatgtc atttaatgga ggaaggatta tatctacatt tgcaatacct gctatagctg
     7321 gtacagcttc tggtacttta ggtatgatga ctatctttta catatcaata gcagtctttg
     7381 tagcaggtgc tattgtgtgg ttattcttac cagagacgtt aaataataag aaataaaata
     7441 ctaggaattt attttgttac ttagaaaggg gtggcttatg atttcatcaa ttgatgtatg
     7501 tattataata ggatatttgc tattaatgtt agttattggt tattattcag gaaaagataa
     7561 taaaaatcaa gaagattatt tcttggcagg taggtcaatg ccatggattc caattgcttt
     7621 atcagttgca gctactatga ttagtgcaaa tggttttata ggtggtcctg gatgggcata
     7681 tgtagatgga atgtatccag taatggttaa tattacagta cctttagcga tattttttgc
     7741 actgagcatt accaccccag ttatctataa attaaaaata acctctatat atgaatatat
     7801 gggacttcgt ttaggaaatt attcgagagg attaactatt attcaatttt ttattaattc
     7861 attgattcaa gctagttcta tggtatatat tccagtgctt attattcaaa tgattactgg
     7921 ctggtcactt tatgctttag tcccaatagt tgtacttgta tcaattatat atactctatt
     7981 aggtgggatt aaggcagtta tatggactga tagcattcaa atgatagtag taataagtgc
     8041 tgtatttata gtaattattg tagccttaaa aggaatgaat ctaagtttct ttgatacatt
     8101 acaaattgca caacagagtg gaaaattaaa tacatttgat ttcaataggg atatttcagt
     8161 tacaaatact ttttgggcta ctttaattgg tggaactgta atgtggataa gatacttctg
     8221 ttttgaccaa tctcaagtac aaagagtttt aacatctaaa tctttaaaat gtgcaaaaaa
     8281 ttcttttgta gttagtgcat ttgttatgaa tttagtttac tactttatgc tattggtagg
     8341 agtaatttta tttgtttttt acgggggaag gacatttgaa acttcaaatg aaataatgat
     8401 tacttttatt ttaaatgaac ttccagtagg agccattgga attattattg caggtgtgtt
     8461 tgcagcagca atgtctagta tcgactctat attaaacagt atgacaactg tttttacgaa
     8521 ggatatttat gagttttatt tcaaaaaaga taacaaagaa tcatcattaa aagttacaat
     8581 gattataact gtagttatag gagttgttat gacaggtttt attattatgg gatttggagg
     8641 aagcattaaa tcaattcttg accttgttgg taactatatc tcatactttg ctggaccagc
     8701 tacaggtgca tttgtactag caatgtttac atataaagct aatgataaag gagtatctat
     8761 tggttttatt gtagggttag ttttaggatt ttttatttct aataaatata atgtgagttg
     8821 gttgtggaat ccagcaatag gtgctacaat aacattgata tttggatata tttttagtgt
     8881 cattttaaag aaaaatcata atgtagatga tattaaaaaa tacactgcat ttggtatgag
     8941 aaatcacctt atttctcaag gaatggataa agtagatgga gtatctatag tgccattttc
     9001 tttaggtaaa caagaattaa ttgtattagc tttttttatt atccaatata ttatattatt
     9061 tttgatacaa ttttaagaag aaatactaga tatttaagga gaaatactaa atgaatcaaa
     9121 agaaagttca ttataataaa tcggctacat caaaagatgt tgcaagattg gcaggagtat
     9181 ctcaatcatc tgtttcaaga gcatttggta gtgcaaatgg aaagggcgta aaacctgaag
     9241 taagagaaag aatttttcgt gtagcaaatg aaataggata tgttccaaat ttggttgcac
     9301 ggggaatgat tagtggaaaa actaatgtta taggattgat agttggtgac agtctaggtc
     9361 cattttacaa tagaattatc aatttatttg tagaaaaaat tcaagagata ggtaaacagt
     9421 gtcttgtttt taaagttccg agacaacaaa gtatagatag tataatttca aaagtaattc
     9481 agtttcaagt agaagctgtg attattacag cttcagccat gaataaggtg atggctgaaa
     9541 cttgtgaaga aaataatatt cctgtgattc tttttaatcg ttttatacct ggtataaaaa
     9601 taagtacagt ctatgtagac ccaattgaag gtgggggcat ggctgctgaa tatctgctta
     9661 agaaaggtca caagaatata ggatatatcc aatttacgag agaaacaagt gaggaaatgg
     9721 aaaagaaaat tggattctat tctaagctaa gacaaagtgg gattcataat cttaaagaag
     9781 aatctgcaaa ctatgattat gattctggat atgaagcagg taagagaatg ttagcattat
     9841 caaatcctcc aacagcaatt ttttgcacaa gtgacttgat agctattggt gtaatggatg
     9901 ctgcaaggtt tgagtataag ttgagggtgc cagaggattt atcggtgata ggatatgatg
     9961 atattcaaat ggcttcatgg aagagttata atttaacgac gattagacaa ccactagatt
    10021 tgttgattga aaaaaccatt aacatactta agtccttatt aaatgaagaa aatccagagt
    10081 cagttgttga aatgttaaaa ccggaattga ttgaaagagg ttctacac
//
LOCUS       DAEORZ010000143         9823 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEORZ010000143 DAEORZ010000000
VERSION     DAEORZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9823
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.146"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(102..659)
                     /locus_tag="KRH39_003131"
     CDS             complement(102..659)
                     /locus_tag="KRH39_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513016.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            complement(687..2213)
                     /locus_tag="KRH39_003132"
     CDS             complement(687..2213)
                     /locus_tag="KRH39_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG8513017.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKAVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKVAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            complement(2593..3600)
                     /locus_tag="KRH39_003133"
     CDS             complement(2593..3600)
                     /locus_tag="KRH39_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG8513018.1"
                     /translation="MIFTIIFYKKGRLCLLNLQSKWVNFLKPTYVSDEIKRKKLQVII
                     FFSITYGLSYSLGLLAICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFY
                     SSVILFFIIYFFIFIIESLKLISINQFQLMYLMLILISSLCVIVYSYKITSLYIFKNF
                     KIGILLFFYFLFSQIIFGLIISDNQFDYKNFLNYILLPIISLVYIYPFLCEEYGWRSF
                     LQSILFDRFGKKIGIVMVGTCWSLWHLPLQFTLYSPQTPIIGAIAHLIYGIGLSIFLG
                     YVYMKTKNIWFCSIMHVLINSLGLIFNDSEIIINYHSIIQRLIFILLFYTPFLFTKEY
                     K"
     gene            complement(4094..6013)
                     /locus_tag="KRH39_003134"
     CDS             complement(4094..6013)
                     /locus_tag="KRH39_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8513019.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAVFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIFLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            complement(6050..6967)
                     /gene="pfkB"
                     /locus_tag="KRH39_003135"
     CDS             complement(6050..6967)
                     /gene="pfkB"
                     /locus_tag="KRH39_003135"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG8513020.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKGGALLITEEEEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKKF"
     gene            complement(7134..8912)
                     /locus_tag="KRH39_003136"
     CDS             complement(7134..8912)
                     /locus_tag="KRH39_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8513021.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            complement(8924..9664)
                     /locus_tag="KRH39_003137"
     CDS             complement(8924..9664)
                     /locus_tag="KRH39_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBG8513022.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
BASE COUNT         3310 a         1698 c          875 g         3940 t
ORIGIN      
        1 taataattat aaataacaaa aatggattta attaatcata tagaccttga aactcaacat
       61 gattaattaa atccattata attaatataa aaaatttaaa actatatatt agattttttt
      121 actataaaag gaatagttat aattgtgcct attaaagcat ctaccatatc aatcatagta
      181 tcttctaacc ctccggcctg tgtagtagtc ccaacaaaag tatccattaa aaattcacct
      241 atttcccata aactcgctac tcccatacta aacataaata aaaatataac taaaaacact
      301 ttactcattt tacttatctc tctttgtgta taaaagtatt taattataga aaaagccaca
      361 atacaagata ttatacctga ccataaatgc aaaaaatcat cataataatt tatatcataa
      421 aaattaaaac aagtacctaa caatgatgaa aacattataa ataaagcaag cattatatat
      481 aattctttac tcaataatct ttccgtttgc ttatttataa aactcaaaat gaaagtacca
      541 actaaactaa ttgctaccag acctagactt ccatattctt tgtttgctac aaaaaatacg
      601 gcagataaaa tataaactat atacaatagt atagttattg gctttaaaag tttattcata
      661 tacactttac cttcctaaaa ataattttat aataacttag gattaacatc attatcacca
      721 tgtccataaa gttttaatgg cttaagagct ggtccttcaa gtatatttcc cttatagtca
      781 aatctagaac catgacatgg acagtcccat gttttttctt cactgttaaa attaagttca
      841 catcctaaat gagtacatgt agtgtctaca atataaaaat ctccattatc atccttatat
      901 acaccatatc tttttccatc aatattaaca atctttcctt ctccattatt tttaggcata
      961 tctccactac ctatttttaa ttttccactt acataattta ttgctacgtt tgcattttcc
     1021 tttataaaat cttttgaaaa gtagctacct aatctggatg gattaaaagt atctttataa
     1081 tcagaattat tatttattat caaatctttt attattattc cagcagcagt tccattagta
     1141 attccccatt tagagaaccc tgtagctaca taaatattgt cttctctttt gttgatataa
     1201 ccaatataag gaacattgtc aaaactcata ttatcttgag cagaccattt ccccttaaaa
     1261 tcttttacct taaagacttc tttaccataa tgctctaaat catcaaatat ttctttctca
     1321 gttcctccct gaccaacttt atggtctcct cctccaaaaa tcagtaaatt ttcacttcca
     1381 tcattatatt gtctaaaagt tattgatggg tcatctatac ttataaacat accatctgct
     1441 aatttatttt ccaaaactgc aaccattaaa taagcacgct ctgcatattc ttttgcaaaa
     1501 taaaaattca atccatcata ccatggacta tgagatgaaa ttataacatt ctcagcttct
     1561 attatattat cttctcttgt tttaacccta catatttttc ctttttcaag gtctactact
     1621 ggtgtatttt catatatttt tagccctaaa tttactgcta cttttgccaa tccatcaata
     1681 tacttctttg ggttaaattg accttgatta gtaaaactaa tagccccctt tatatctaat
     1741 ggaatatttt ctatagtttc atgatactca caatttattc ctattttttt acatgtacga
     1801 tattcatctt taatattctt tatagtatcc tctttttctg taaatacaaa agaagtttcc
     1861 cttttaaaat cacattttat attgttttct tctattattt gttcaactaa atttaaagct
     1921 tcattatttg cattataata tgattttgct ttatctaatc catatttttt ctctatctta
     1981 gagtaaaata tatcatgctg acaagttacc ttaccagtat tccttccaga agttccatat
     2041 cctattttat cagcctcaac aagcactgct tttttacctt cttttgctaa taaatatgca
     2101 gttgtcaatc cagttattcc accacctact ataagacaat ctgtttttat attttcacct
     2161 aatttgttat attcttttgg acttgagttt aaaaaccaat atgattcatt catttaaaat
     2221 actcctttac atattaaata gtaatagttt tcccatcatg gagtttattt attataaata
     2281 gtataaaaaa taagcttaat aaaaagctaa attaaaaatc acttagtttt cttgttttat
     2341 acaccagact aaacttatat atatttataa tttctatatt attttcttaa ttaatagtat
     2401 ttatgctaag taaaaatgag gatattttag atttcaatac ataagatgat gttgtgctaa
     2461 caattataaa ctgttagcca ccaacttttt tattgattta gttagtttaa tatttttctg
     2521 atatgtaata tagcaatctc tctataaaaa tatttactta aaaatttatt ttatataaat
     2581 taattgtaat tcttacttat attcttttgt aaatagaaaa ggagtataaa ataatagtat
     2641 aaatatcaat ctttgtataa tactatggta attaataatt atttcagaat cattgaaaat
     2701 taatcctaag ctattaatta gaacatgcat tatagagcaa aaccatatgt tcttagtttt
     2761 catataaaca taacctaaaa atatagaaag tccaatacca tatatcaaat gggctatagc
     2821 tccaattatt ggagtttgag gactatatag tgtaaattgt aaaggtaagt gccataaact
     2881 ccaacaagtg cctaccatta caatacctat ttttttgcca aatctatcaa ataaaatact
     2941 ttgcaaaaaa gatctccaac catactcttc acataaaaaa ggataaatgt atactaatga
     3001 tattatagga agaagaatat aattcaagaa attcttataa tcaaattgat tatcacttat
     3061 tattaatcca aaaataattt gagaaaataa aaagtaaaaa aatagcaaaa tacctatctt
     3121 aaaattttta aaaatatata aacttgttat tttataagaa taaacaataa cacataaact
     3181 acttattaaa attaacataa gatacattaa ttgaaattga ttaatactta ttaactttaa
     3241 tgattcaata ataaatataa aaaagtaaat aataaaaaat aatattactg atgaataaaa
     3301 attatattta tcattatttc ttccctctgt gtaaaatttt gcaatagaaa ctgaagataa
     3361 tggcaatatc atcataaatt gagctaaaat ttctgaatct attaaatgat taaaacatat
     3421 tgctaataaa cctagtgaat aagataaacc ataagttata gaaaaaaata ttataacttg
     3481 tagttttttt ctttttattt catcactgac atatgttggc tttaaaaagt ttacccattt
     3541 tgattgtaaa ttcaacaaac ataacctccc ttttttataa aaaataattg taaaaatcat
     3601 aaaatttttc tacttatact catatcatta tatttctata tttataaagt ccttattact
     3661 tacacgactg ctgactaaac tcttataatt ctttttttga atatgtattt acagaagaaa
     3721 tatccaattt cgttagaaat ttattcttct gcttaaattt tatataaaaa tcatttttat
     3781 gcaatcagtt tactattatt tttttaatat tacaatatat tttatataca aaaaaataca
     3841 acaaatatta ttttatagtt cgagtgaata ttaaaataat atataatttt ctttatgttc
     3901 taacttgaaa acaatttaaa tacaaataaa gcaaaacaca cttattttac ttatataaaa
     3961 ataaaagact gtcaagataa caattacaaa tcgttatata acagcctcat aaaatcctta
     4021 ttttaaaata attcagcaat atattaattt ttataaaaca tcatatttta agttcttgaa
     4081 tatcctttaa ttgctatact tcttgattta atggtttttt aagtaatgct aaaagcatca
     4141 ttccaaccaa agcgcctaca gctatagcta atatatatgc aaatggatgg gtaaccactg
     4201 gtataacaaa tactcctcca tgaggtgcac gaagagctgc gttgaacaac atactaagtg
     4261 ctcctgccac tgctgaacct ccaacacaag ctggtataac atgaagtggg tctccagctg
     4321 caaaaggtat cgctccttca gtaacaaatg ataatcccat tacataatta actttaccag
     4381 aatctctttc ttctttagta aatctattct taaaaaatgt agttgcaaga gctatagcaa
     4441 gtggcggaac cataccacca gccattactg ctgccattat ttcaaactgc cctgattcaa
     4501 gtgatgcaac tccaaataca tatgctgctt tatttacagg tccacccata tccacagcca
     4561 tcattccacc taatactaat cctaaaaata tttttgaagt tccacctaaa ccatttaaaa
     4621 atccaaccat tgcattatta attgctgtaa ctggaggtat tactaaaaat gtcattataa
     4681 ttcccatcaa aagtgttcca agtaaaggat ataacaatac tggttttatt ccttccaatg
     4741 aagctggtag tacactaaat attttcttaa gcccttctac taaataacct gctataaaac
     4801 ctgcaagtag tgcacctaaa aatcctgaac caccatcacc tgctagagca cctcctacaa
     4861 atcctacaac aaacgctggt ctatcagata tagagtatgc tatatatcca gcaagtacag
     4921 gtaacataaa tccaaaagct ttatctccta ttcctttaaa aaaaactgct ataggtgtat
     4981 ttgaaccaaa gtttgaaggg ttaatagtat aatcatcaag taaaaaggct atcgctataa
     5041 gtatccctcc accaataaca aatggtaaca tattagatac accattcatt aagtgtttgt
     5101 atacttttct aaatccactt tcactttctt catttgattc gttgtgactt ccatctcctc
     5161 catggtaaat tggagcatct ccatttacag ccttgtctat aagttcttcc gctttgtgta
     5221 taccatcagc caccttagtt tttatgactt ttttgccatt aaatctagcc atctcaacat
     5281 ttttatctgc tgccactatt atacatgttg cattttctat ttcatctttt gtaagcttat
     5341 ttttaacacc tgctgaacca ttagtttcta cttttattga tacacctttg ttttctgcca
     5401 ttttatttaa actttcagct gccataaatg tatgtgctat tccagttgga catgctgtta
     5461 ctgctaaaac cttaggatac ttattagcat cattctcttt agtatctatt atattttttt
     5521 cagtaacttt ctcatcttga tattcttcac tgaatttttc tctttccttt ttatctatta
     5581 tttccttaaa ttctttttca gagcttgaat ttataagttt atttctaaag tcttcatcca
     5641 taagtattgt agataatctt gataatactt caagatgagt attgttagct ccgtctggtg
     5701 cagctatcat aaaaaataga tttgataaac ttccgtcaaa cgattcataa tctatacctt
     5761 cttttgacac tgctgctgct atacaagctt tagtaactga agaattttta gcatgaggta
     5821 tagctacacc ttctccaatt cctgttgtac tcatttcttc acgagccaaa attgcttttt
     5881 tgtagttttc tttatcattt aaatttccac tatttgcgac caaatctact aattcttcta
     5941 ttgcttctga tttggttttt gaatttaaat ttaacttaat tgacttttca tctaataaat
     6001 ctataatttt cataaatatt aattaccttt ctatatattt tttaatattt taaaacttct
     6061 ttacagatat ctcatttacc aatttatcaa tgtattctct tttggctaag tcatatgaaa
     6121 aagcagttgc actacctgaa gctgctccca atttaagagc ataatcatat ttcccagtat
     6181 tcaaataccc agatataaat cctgctacca ttgaatcacc tgctcctaca gaatttttta
     6241 cttttccttt tggaacatca cttataagca cttcttcttc ctctgtaatc aacaatgctc
     6301 caccttttcc cattgatacc aagacattta tagctcccat ttcctttaac cttctagcat
     6361 attttaccat gtcttcaatt gaatttaatt taacaccaaa tagttcttct aattcgtcgt
     6421 tattaggctt aattagaaaa ggtttgtatt ttaaaacatt aagaagaaga tttttagttg
     6481 catctactac aacttttata ttcttttttt ctaatctagc cattatattt tcgtatagtt
     6541 tttcatctaa agtagatggt atacttcctg ccaatattaa aatatcatct tgatttagct
     6601 tatcaagttt tttatataaa gtatcaatgt cttcatcatc tatatttgga ccttgtccat
     6661 taatctcagt ttcctttaac gcctttattt ttacatttat tcttgaaaaa ccacttttta
     6721 tctttataaa atctgtcttc aattcttttt cttccaatgt tcttattata tattctccag
     6781 taaaaccact tataaaacct aaagatgtgt tatcatttcc aagttcctta agtatgcgag
     6841 atacatttat accttttcct cctggataaa cgtattcttc ttttacacga tttgtacttc
     6901 ctgtctttag ctcatctagc ttaactatat aatctattga tggatttaat gtcactgtat
     6961 aaatcatatt gccacctctt ttttatttat ttgctttatg tttatatctt aactttatat
     7021 taatatcctt gaaagtcaaa aaatttctct taattaaaag agaaatttat cgtttttatc
     7081 ttatttagtt ttatattatc caaatttaca ggtgtatata caatactacc catctaatta
     7141 aataaattta caaatttttc aaaactacta ttttcacata tacttataac ttttgctata
     7201 gaatctactc ttttatatat ttttgaaaat attacttcat aatctaatat tttatcttta
     7261 tcaagggcta acattattat taaacgaaca tctgatattt cccaagaaat aggcttcttt
     7321 aatataccaa ttgctatttt attatcaaaa atttttccaa tagctccatg tggtatagtt
     7381 accatattcc ctatttctgt tgttgctatt ttttctctct caaagtaaga ttttttcata
     7441 tcctcatcta tatagccctt ttgtacaagt ttacttgcca taaaatctat aacttcttct
     7501 ttccttttaa acaccaaatc agtaaaaaac aattcactat caaacaggct ttttatatct
     7561 aaatctaggt atatatttcc tgtttctata tatttttcaa ttaatctaat ttctttctca
     7621 cttaaaattg gagatatatt tattactggc acatctccta cttcaatagg aacagtagat
     7681 attacaaaat caacatctat aacttttata tattcaacta agtatgaagg aataatcttt
     7741 agtatttcta atttttcctt aaatatattc tctaatttag cttttattaa cattgatgtg
     7801 cctatgcctg aagtacaaac tattatagtt ttgaaaacct tatctttatt attataagat
     7861 gctctttcaa ctgctcctcc tatatgtagg gctatgtttg caacttcatc ttcattaatt
     7921 tctatcttca aatctttttc tattgtcttt ttagctatat tagctagttc aaaagcaaat
     7981 ggatatttta tttttatatc ttgagccatt ataggtgatt gatttatacc taactttatt
     8041 tttttacata aactactaat atgcttatat aaaaactctt caaatacctt atcattactt
     8101 atatctattg atgtagctaa ttttaaattt ttacaaaatg tttttattga atttattata
     8161 atttctttgt tttgttcatt ttctaaaatt gagcttcttt tgctcctata attttctata
     8221 taattctcta taaattcttt ttcattatac ttatgtctgc ttaacataac taatatatta
     8281 tttaacacac tcttaaattc tatatcagtt aataccaatc cattttcatt taatatgtct
     8341 aataataatt tttttattaa tagttttctt tcctttatat catcatttaa aaaatcattt
     8401 aaactcaatt gattatctct cttattgctt aagtcaacta tacaatttaa tatcttatct
     8461 tcactttcca aaacacatat accttgttta tgcttagatt taactgatat tgaatacttt
     8521 tccaatattc tttttacttc ttttaaatca ttttttactg ttgaaacact tacatacata
     8581 gaatcggcta aatcttcaag ttttatagta tttttttctt cattacttaa ggttaatatt
     8641 tttctgatta tatattctac tctatctaca tcatcataga gattgctatt tagatttacc
     8701 tcatcaaaac ttccttctag tttataacca actcctctta ttgacaaaat tttaccattt
     8761 ctaagttgtt tattaatatc ctttatttca ttttgtatag tttttgaaga acatcctatc
     8821 tcttgactta aggcaatacc actaattgta tcttttgaat ttaacaatat ttttattata
     8881 tttatctgtc ttgctgttat atttaaactc ataaactcac cttttactct agatattgat
     8941 ttcttaattt tcttatttct tctttattta aattaaattc tccatataaa tatgcttcta
     9001 cactaatgta ttcttcatca attcttctaa atgcagtttt catatactct tcatttacac
     9061 caaatatata tttcaaattt tcttttggaa tgtccttaaa tttaggctta tattctataa
     9121 atctttctat ctctttatct gcaaaatcat tactttttag ataatctttc attatattct
     9181 ctctcgatac ccctaaaatc ataagaatta tagcactccc cacaccagtt ctatccttac
     9241 cagctgtaca atgatttaat attggtaaat tagaatagtc tcttataagt tctactaatt
     9301 ttttatatga cggattattt ataggcaaat tatagtaact attatttaac atattaaatg
     9361 caccatcttt ttcaaacaat ccatcaatca tatcttcaat agaaccaaaa cttcctcctg
     9421 attcttctag ctctctcatt gctggtattc ttatattttt tatatttgat attattgttg
     9481 atggatgttt acgagcttct tcatcactcc tataatcaaa tatatattta atatttaact
     9541 cttttaaaat ctttatatca ttttcactta gatttgataa ttttgcagac ctataaaata
     9601 atcccttctt tacagttctg ccatcctcag ttttgttccc acctaaatct ctaaaatttt
     9661 tcatcttagc acctcttaaa aaccatatat tatacttttt ttaatatcta taaattaatt
     9721 ttatcacaat tttaagttct ttatttaaaa tattttttag tatttggtta ttaattgtaa
     9781 aatataaaag tatctcttta tacaaaatta acctaaattt tac
//
LOCUS       DAEORZ010000144         9803 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEORZ010000144 DAEORZ010000000
VERSION     DAEORZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.147"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..613)
                     /locus_tag="KRH39_003138"
     CDS             complement(<1..613)
                     /locus_tag="KRH39_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8513023.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLK"
     gene            complement(640..2481)
                     /locus_tag="KRH39_003139"
     CDS             complement(640..2481)
                     /locus_tag="KRH39_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8513024.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            complement(2612..3031)
                     /locus_tag="KRH39_003140"
     CDS             complement(2612..3031)
                     /locus_tag="KRH39_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBG8513025.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            3344..3526
                     /locus_tag="KRH39_003141"
     CDS             3344..3526
                     /locus_tag="KRH39_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513026.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            3856..4878
                     /locus_tag="KRH39_003142"
     CDS             3856..4878
                     /locus_tag="KRH39_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG8513027.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DAFMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(4963..5136)
                     /locus_tag="KRH39_003143"
     CDS             complement(4963..5136)
                     /locus_tag="KRH39_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG8513028.1"
                     /translation="MHLVQLLCQQINGSDIDPDSKFGPATYNQVKILQGRLGVTKDGV
                     VGKATWQAAGERY"
     gene            complement(5615..6649)
                     /locus_tag="KRH39_003144"
     CDS             complement(5615..6649)
                     /locus_tag="KRH39_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBG8513029.1"
                     /translation="MNNYMLYVFDNLIKTTIVCSFGICLLLFLKRYFFKRFSKRFNYY
                     IWLVIVFRMLLFLFNYTVMYEVKEPKENTVGNNITQIDVSIDNDFMLYLVYLWLFVTI
                     AIAVYTFIEYTKFKNLVVDVSYDIEDNDVNCIYKNLLKELDIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYTYSKDELTWILKHELMHFKNKDILIKFLVLSVKIMYWFNPLVYI
                     MSNKVNLDCELCCDESVLTDCSLKDKKAYALALIKSIKLSKNYNSGILTTEFNKTNLE
                     KRLDSIIKKKGKSGILMLLILFTLFSVTYFNFESISFDNSNYLRNIGILEKTGADLKE
                     EDDISMRVVN"
     gene            complement(6642..7019)
                     /locus_tag="KRH39_003145"
     CDS             complement(6642..7019)
                     /locus_tag="KRH39_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8513030.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDATVTSKDVIEVMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTVIVKHKDYLSFETKDFLSNIHDDSLKSLISALH
                     DDENIGKDTLDFLESYFDNLKNE"
     gene            7504..8749
                     /locus_tag="KRH39_003146"
                     /pseudo
     CDS             7504..8749
                     /locus_tag="KRH39_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614087.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(8920..9507)
                     /locus_tag="KRH39_003147"
     CDS             complement(8920..9507)
                     /locus_tag="KRH39_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG8513031.1"
                     /translation="MKNLKKILIITGCLTLISTSLVFANTTTDVDTSKDDDSIISSCT
                     ITGYEFTYENAPQSVKFDYEKKCEALNIKPSKNDIIFVNKDSIDANDVENYVEPKAYI
                     IRYKKGYVFEVTGPKSYTLNVSNTVVGYNRVESGNPVHLVQLLCRTLDGSSIDDDSEF
                     GPATYNAVKSLQGRLGLTRDGIVGRSTWDAAGRRL"
     gene            complement(9573..>9803)
                     /locus_tag="KRH39_003148"
     CDS             complement(9573..>9803)
                     /locus_tag="KRH39_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBG8513032.1"
                     /translation="EFNKTSLEKRLDSIVKKKGKSGILVLLILITLFSVTYFNFESIS
                     FDNSNSLRNMGILQKSGVDLKEEDDISMRVIN"
BASE COUNT         3537 a         1352 c         1063 g         3851 t
ORIGIN      
        1 attttagaca ttcaattaag tattccatac tttttataaa gttattaaat aaatatagat
       61 aaactctccc aaatatataa tttgccctac ttacaagagc tttatttttt aaagttttaa
      121 aaaaagacac ttcttctact aaagctatta catccgtttt ggtctgatat aaagcactta
      181 tttctacaca taaactcata tttaacaata ccaacaactc atttaaatga ttttgtctta
      241 taattttttt tgctgagaaa taaaaattat ttaccttatc atattttctt atagaaatag
      301 ctgataaagt aagataaata taaattttag atattatatc tgaatcatct tttaatgaca
      361 attttttaat atactttaat gactctatca aatagttgta tgatgcttct ctatcttttt
      421 ttatatagtt tataagacta attaaatata aagtatagac tctactatta tcttctaatt
      481 ttgtatttaa aaggtctttg tgataatcca agatgtagtt taaatctgtc aaatttagat
      541 agaatttagt caacatatcg tattctatct tgtttatatt tctctttaaa tcttctatta
      601 aactcctttt caagtaaatc accaatccta tttcaacagt taaaaattct taaaatcatg
      661 ataatgcaaa catctatttc taccactttc ctttgcttta tataaagctt tatcagcatt
      721 ttcaattatt tcttttatat ctatactact atttttacat gtatcaatgc caatactaac
      781 tgtcaaatta ttgtttaaaa atttctttac aatcctacat aattttcttc caaatatttt
      841 acttttttta taatctttac atacagataa tatcagaaat tcttctccac cataccttat
      901 cataagcatg tcttttttta gatatttttt tattattata gttatatttt taagagcaat
      961 atctccattg tagtgtccat aagtatcatt atattcttta aagtaatcta catctatcat
     1021 aaatgctgtg caatattcac taacttcttg tttttctaaa aaatcgttca aaaaagttct
     1081 attatatgca cttgtcaaat ggtcaacata ctttttttta tctaaatcaa gttttacttt
     1141 tctcatgtta tttatttctt tttctatata ctcatgtcta taatttaagg aatctatata
     1201 atttctattt cgagctttta tatacatcat ttttaactca taatacattt taaagtattt
     1261 catactcatt tcataatttt gaatcttttc aaaacacaaa tatatactct tgtatgctaa
     1321 cttcacataa ttataatttt tagcattttt agatttttca aggagttttt tatttgtgtt
     1381 taaggcatta tcataatatc caaagatata atatatcttt gcctggtgat aaattatttc
     1441 taaatcatat attatatatt cttctatatt ttttatcata tttaatatgt ttttagctct
     1501 atctaaatag catattgatt gttcaaaaga tttttcatta ctttttgcat aatacatggc
     1561 taaacttata aataaatcaa acttctctac ttcttttaag ctatgatttt taattttttc
     1621 tagatatact tcagattttg ctaaattatt tattgcgaca ggataatttc tagtctttat
     1681 taatatttta gttatgttgt ttgatgctct atatttttga attatattta catttctata
     1741 ctttttattg cttattactt cattgaaata ttccatagaa cattcataat tttcaaacga
     1801 acattcacac tcacctatct ttatattaca tagtgaagat acttgcatat cctttagtaa
     1861 atctgcatat tttcttgctt ttaaaaaatt taccttagca attagagcat catccaaaaa
     1921 caaaaaatat attttaccaa taataaaaaa atatattttt ttacaattaa aatcgttaat
     1981 ttcttttatc tttatataca acgtttctat ttctttaatt attacagaag attctataca
     2041 cttacttgcc ttaatgtaaa taagttgtga tttaaaaata atttcttcaa ttagaaaatt
     2101 attttcatca caaataagat taaatttatc catatataaa tttgccttat ttacatcttc
     2161 attttttaaa cttaaaatag ctatttgtga ataaaatcgt gctaaaaatt tataatcttt
     2221 gggtaagcat ttatcaatat atttacagga aatcaaaata tactctttca ttttttcact
     2281 aaacccttga ataccatata ataaagctag caaaaaattt gattttaata acattaaact
     2341 attttgttca gttttatagc aatcaattaa attctttact acatcaattt tattttcaat
     2401 aaaatcgtta ttagtcaata atctttcatt caaggttagt ttaaacattg aatctaaaat
     2461 tgtatcatct atctcttcca aatatttact atatattgta actcacctcc gacttacatt
     2521 atctcataaa tttagtaatt ttccatacta tttttgaaaa aagttgatgt ctcaatttag
     2581 aaatttctct taaattaaga catactttac actaaactat acaattttct atctgaacta
     2641 tataatcctg acaattttcg caaattatca cacttacaat ataactatta tcaactttat
     2701 ttgcccttga tagtttatta aaagatagtc ctttataatc aacagatata ttgcttatta
     2761 catattctcc atctttactg aaaatataaa agtgatattc taaaaaagga aagaattttt
     2821 tcttttcttt tactccttct aaacataatg gacatgcttc ttgataatat tctacatcaa
     2881 aatcaacttc ttctgcttca ttaagtatat caattatttt tgaaatactt actccttgaa
     2941 ctggattttc ttcatcattt attaaagcac tacagttctc ctcttttaat aaatattcct
     3001 tatcatcata tataaactta tataaactca tattatatcc tccttataat tattaatctc
     3061 caagttattc taccatttaa ataaagttaa taaaagtaat tgtttataat ctttataatt
     3121 aagttatgta tacatatatt cctatgcgct atcttaatta atgattttaa aatccaagtt
     3181 tgcactttga acatccagtt tcccaaaatc tattgtcaaa actcacttta gttattaata
     3241 taatgatata aatatataac tgcatagatg ttacacactt aactatttaa tatgatacga
     3301 tttggtatct aaatttatat attgtatata gggggagata tatatgtata aagataaaag
     3361 tgatgaatgt atacacttaa tgactgcata catcgatagt ataagtggat attattcatt
     3421 tattgacaca caattagagg attttatgat gaaatatggt gaaaacattg ttgattcaaa
     3481 tttacacagt ataatgatgt tgctttgtaa atggggatta tcttagcatt tcaagaaact
     3541 tttttgtata ttgtattcta tattatttat tcattaacaa aaaaagattg acataaactt
     3601 aaaagctata tatagatttc tttaaaaatc tatatatagc ttttttaatt ttttcaaaat
     3661 gtatacttaa ataagttagt ttttttcaaa atttgactat ctaatatgat ataatttaac
     3721 tatctacaac aataaaaaat atctaatatg tattatctag tttagctcat cataataaca
     3781 tccccatatt tacaattaag atattttcaa tatagtaata ctcaatttta atattataaa
     3841 aaggagagat tttgtatgaa ttacaatgac ttactaaaat cagctagaga gaactttaat
     3901 ggaacatgta aagtatgtaa aatttgtaat ggattagctt gtgctggaga tgttcctggt
     3961 atgggtggta aaggaagtgg ttcgtctttc attgaaaata gaaaaagtct agaaaaaata
     4021 aagataaata tgagagtcat acataatgtt tccaagccag atacatccat agaattattt
     4081 ggtagaaaaa tgagttctcc tatattcgct gcacctgtat caggtactat attgaacatg
     4141 ggtggtaaag tttccgaaaa agaatacata gagcctgttg ttagaggttg ttctaattct
     4201 ggaatttatg ctatggttgg cgatactaat gtagatactt ttttactgga taatttagat
     4261 gtattgaagg ataatagggg aaatggaatt gtatttataa agccttggaa taattcaaag
     4321 ataatagaaa aaattagatt gtctgaagaa gcaggtgctt ttgcagttgg tgtagacctt
     4381 gatgcttgtg gacttattaa taatcaattt caagaaaatc ctttttctcc taaaacaatt
     4441 gatgagatta gagaattggt tgaatccact agattacctt ttattataaa aggaattatg
     4501 actgtagatg atgcttttat ggctgtagag tctggtgcta gtgccattat tgtttcaaat
     4561 catggtggaa gagtacttga ttatactcct ggtacttgtg aggtacttcc agatatagct
     4621 aagtctgtaa agggaaagat aacaatactt gttgatggtg gagttaggac tggtgttgat
     4681 gtagttaaga tgcttggatt gggtgctgat gctgttttga tgggaagacc ttttgttact
     4741 gcttcttttg gcggtggtct tgatggtgtg gagtttttta tagagaaggt taggaatgag
     4801 ttatgtgaga ctatgatttt gactggttgt caaaatgtta aggatataga tggtagagtt
     4861 atatggaatt tattgtaaaa tatttctaga ctctaagata ctgattaata tattctatat
     4921 aaaaaggttg tcgccctatt tcttagtgcg acaacctctt ttctaataac gctctccggc
     4981 agcttgccaa gtggcttttc caacaacacc atcttttgtt acaccaagtc ttccttgaag
     5041 tatttttact tgattatatg tagcaggtcc aaatttacta tctggatcaa tatcagaacc
     5101 atttatttgt tgacataaaa gttgtactaa atgcacagga ttcccttttt ctacatgtcc
     5161 atatcctact acagtggtat ttatatttac gctataactt ataggaccag taacatcaaa
     5221 acgatttaaa tcttttttat atctaataat aaatgctttt ggttcaatat tataatcatc
     5281 tatattaata ttatatgaat ctataaattt ttcattaata aagattgtat catctttaga
     5341 tggatttata tttaattctt tacatttttt ttcataatca gattttatag gttgtggtgc
     5401 attctcataa gtaaattcat aaccagttat tgtaaatgaa tttgttgttt gctcttcatt
     5461 actttttgat acatttacat cattattagt acttgcaaat actaaactag tagatattag
     5521 agtcgccctc cctgtgacta ctaagatttt tttagatttt taatttcaaa acttctccta
     5581 atttgttaat aaattataat actataaaaa tctattaatt tacaactctc atgctaatat
     5641 catcttcttc cttaagatct gcgcctgttt tttctaaaat ccctatattt cttaaataat
     5701 ttgaattatc aaaactaata ctttcaaaat taaaatatgt aactgaaaat aatgtaaaca
     5761 aaatcaataa catcaatatt ccacttttcc cttttttctt aataatacta tcaagtctct
     5821 tttccaaatt tgtcttatta aattcagttg ttaaaatacc actattataa ttcttactaa
     5881 gtttaattga ctttataagt gctaacgcat atgctttctt atcttttaaa gagcaatcag
     5941 tcaaaacact ttcatcacaa cataattcac aatctagatt tactttattg ctcattatat
     6001 aaacaagtgg attaaaccaa tacattattt taacagacaa aactaaaaac tttattaaaa
     6061 tatctttgtt tttaaaatgc attaattcat gtttcaaaat ccaagttaac tcatctttac
     6121 tataagtata gtctggtaag aatatgtacg atttaaataa tcccatacct gctggactta
     6181 tcaattcatc agaacccctt aactcaattt tcttttttat gtctaattct tttaacaaat
     6241 ttttatagat acaatttaca tcattatctt caatatcata agatacatca acaacgagat
     6301 ttttaaattt tgtgtattct ataaacgtat aaactgcaat ggctatagtt acaaataacc
     6361 ataaataaac taaatacagc ataaaatcat tatctataga aacatcaatt tgtgtaatat
     6421 tatttcctac tgtattttct ttgggctctt ttacttcata cattactgta taattaaata
     6481 gaaaaagtaa cattctaaag actattacca accaaatata ataattaaat ctcttactaa
     6541 acctcttaaa aaaatatctt ttcaaaaata ataatagaca aataccaaaa ctacatacaa
     6601 tagttgtctt tataagattg tcaaaaacat atagcatata attattcatt ctttaaatta
     6661 tcaaaataac tttctaaaaa atctagcgtg tctttaccta tattttcatc atcatgtaat
     6721 gctgaaatta agcttttcaa agaatcatca tgtatattac ttaggaaatc ctttgtctca
     6781 aaacttaaat aatctttgtg ttttactatt actgtgtaat gtgtatatct gtctattttt
     6841 tgagcatcta agaatcctct ttttactaac ctcgataaaa gtgttagtgt agtggtttgt
     6901 ttccaaccat atttttgttc cataacttca ataacatctt ttgatgtcac tgtagcatct
     6961 actttccaaa taaatttcat tacttttagt tctgcttgtg gtatttttct tatcatcatt
     7021 tacacctccc tcaaaactct tgctacttta agtctacaat atgtgaattt tttagtcaaa
     7081 cgctttatga aaaaatttga atttttgtac tttttattaa ttttctaatt tttttaaata
     7141 ttaaaagtta gattttaaaa tatagttgtt caagtatttt gctttttaca tatttaatta
     7201 taatagtgta gaatgttatt tccgcattaa aattatttaa caaaacgccc tatattttta
     7261 cacaaggaat attataatac tttttttaaa ataaactaga gtaatatcta ataaatttta
     7321 ataatctcct aattgtacct ttgtgttatt aaaatatctg ttttatataa gtttatgtta
     7381 taagtaaaat tatacataga aaataaaggt ataaaaacac cattttcagt gtctttaaaa
     7441 taaagtgtgt attcttctaa aaagttatga taattaaaat atcactacta tacaaaggaa
     7501 gatatatact ttttatgttt aacaaaaact aaaatataaa aaaaacttat tgaagaatat
     7561 gatgtcaaaa caactacaga tattcaagat atactaaaag atttatttga aagtacaatc
     7621 cattaaatgt tagaagcgga gctttatgaa cttttaggtt atgatagaat gataataaga
     7681 acaaaaatac taaaaactct agaaatggat atagaaataa aaatattaaa tcagattttg
     7741 gagaaataac tttaaatatt tcaagaaagc gaaatgaaga ttttgaacta aaggtaatta
     7801 gaaactatga aaatgatata ttaggaataa aaaatcaagt gattggaata tactcaaaag
     7861 acatgtctac tcaaaatata ttaagtcatc ttaaaagtat ttacggaatt gatattatct
     7921 cattcaataa taactaaaac tactgataaa gttattcctt taactcataa ctggtaaaat
     7981 agacctctta atataattta ttcaattgta tttatagatg ctatacatta taaagttaga
     8041 agtgaggtag aataataaac aaaacagtct atatagctat atgtattaac ttagaaggca
     8101 taaaatgggg tttaagaata tggattggag aaaatgaaat ttcaaagtat tggctaaaag
     8161 tgcttataga tgctagaaac atgggaatta aagatgtatt aatagcagct tctatatata
     8221 gactagttga attttctaat gcaattaaag cgatatttcc caatacttaa gtacaaagat
     8281 atgctgtaca tcaaattaaa aatactttaa attatgtatc atataaaaat agaaaagaat
     8341 ttgtaaaaga tttaaagaaa gatatgctac aattacagaa gaggtagcat taagtgagct
     8401 atcactagag aaaaaatagt acaatcaata tacaatagta ttaagaggtt ggtgaaacaa
     8461 ttggaatgaa ttagtaacat attttaagta tcctcaagaa ataaatacat caatatatac
     8521 tacaaattca atgggaagtt agaatagatt attaagaaaa gttactaaat tgaaatctat
     8581 ttttacttta gataattatt tgcacaaatc actatactta acaaccatgt atatttctta
     8641 aaaatgaact cagaaaatta gaaattgtgc tcaaatttta gctcatttaa gtatttattt
     8701 tgaagaaaga aatttttcaa aaaaacaaga aattttctta aagggttaat agcttttgca
     8761 aaaatggaaa agatatcaaa ataaggctat gatttttaaa gccatagccc tattagtaaa
     8821 aatttaatta tcacttaaaa aataaatttt tgtttaaaga atacatgtaa atgtttacag
     8881 attcagcaat ttaaaattat ctgtgcaata gctttctttt taaagacgtc ttccagctgc
     8941 atcccaagta cttcttccaa caattccatc tcttgttaat ccaagtcttc cttgaagtga
     9001 cttaactgca ttatatgtag caggtccaaa ttcactatca tcatcaatac ttgaaccatc
     9061 taaggttcta cataaaagtt gaactaaatg cacaggatta cctgattcaa cacgattata
     9121 tccgactaca gtattactta catttaatgt ataactctta ggtccagtaa cttcaaaaac
     9181 ataacctttt ttatatctaa taatatatgc ttttggctca acatagtttt ctacatcatt
     9241 tgcatctata gaatctttgt taacaaaaat tatatcattt ttagatggtt ttatatttag
     9301 cgcttcacat tttttttcat aatcaaattt tacagattgt ggtgcgtttt catatgtaaa
     9361 ttcataacca gttatagtac atgaacttat tattgaatca tcatcctttg atgtatctac
     9421 atcagtagta gtatttgcaa ataccaaact agtagatatt agagttagac aacctgtaat
     9481 tattaaaatt ttttttaaat ttttcatttc ataattcctc ctaatttatt aataatatga
     9541 caatatacct tttcattaca tatattaatc tattaattta taactctcat gctaatatca
     9601 tcttcttcct taagatctac tcctgatttt tgtaaaatcc ccatattcct taaagaattt
     9661 gaattatcaa aactaatact ttcaaaatta aaatatgtaa ctgaaaataa tgtaatcaaa
     9721 atcaataaca ccaatattcc acttttacct tttttcttaa caatactgtc aagtctcttt
     9781 tccaaacttg tcttattaaa ctc
//
LOCUS       DAEORZ010000145         9690 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEORZ010000145 DAEORZ010000000
VERSION     DAEORZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.148"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(79..3060)
                     /locus_tag="KRH39_003149"
     CDS             complement(79..3060)
                     /locus_tag="KRH39_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBG8513033.1"
                     /translation="MLPKEKHLEESIENHLVSIGYEQGHSQEFDLENCLFVEDLFRFL
                     EITQKELLDEFKYNRGSNYKKAFIDLINSNIKRRGLISVLRNGVEDIMMNGKFQLFYN
                     KPNLTTNKTAYENYKQNIFKITRQLKYSSKHNNTLDIVLSLNGFPVIVMELKNQFTNQ
                     NVYNAIKQFKQDRNPKETLFGFNQRVFVYFAVDTDEVYMTTELKGDKTFFLPFNKGNN
                     MGKGNPVVEGKEKTSYLWEEILTKDSLLDIIKRFYFIQEDDKKSKRAIFPRFHQLDAV
                     RKIVADLEVNKAGKNYLIQHSAGSGKTNTIAWTAHRLSSLHDEDNNAIFDSVIVVTDR
                     KVLDKQLQDAIYQLEHKNGLVVKIDDDKNSSDLADAIVNRAKIIITTIQKFQYALPKI
                     EQLEKRRYAVIIDEAHSSTSGENMNALKETLAGKTLEEAKIIDAENEKNEKSSIDKMN
                     DLIEKRTNLDHISFFAFTATPKNKTMQVFGRIGEDGLPHEFHLYSMKQAIEEGFILDV
                     LKNFMPVSVYYKVGKKIEDNPEFDKGEAKRAINKFISLNEHNIRQKVETIVDDFVDNR
                     SMWLEGRAKGMIVTASRLHAVKYKLAIDQYIKEKGYNMKSLVAFSDTVKDEDIEYTEV
                     GMNKEVAPDINDSNLPSIFEKSDFRLLIVAEKYQTGFDQPKLCAMYVDKKLDGVKTVQ
                     TLSRLNRTYPNKHTFILDFQNSVEDIQEAYKPYFEMTNIDKVTEPNVVNDLWYQLHEY
                     GIYTDEEVNDFAVLFYKDKRTNTQDAKMNSLIDKAVERYCYLDEEDEKSADEFKKKCQ
                     KYITFYNFLIQIYPIKNLNLLRLQVYLVALLRKLPKKGKERIDIDDILSLDYYKLQKL
                     GEDGKKGSDISLYNGEGELVGISETATSRVSEADEEYLEDIIQKINDVFGVGLTEEDR
                     IIIDQYEEMFKNDKSIMDIAKANSYEDLVSTFGKSYFKRGIVKTKNMNDRLVKEILTN
                     NNLQNYMIHYLAEKIYAEANR"
     gene            complement(3122..4023)
                     /locus_tag="KRH39_003150"
                     /pseudo
     CDS             complement(3122..4023)
                     /locus_tag="KRH39_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4099..4419)
                     /locus_tag="KRH39_003151"
     CDS             complement(4099..4419)
                     /locus_tag="KRH39_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513034.1"
                     /translation="MLYVGDYIDMKTLNIRYKDGFKIEEDTRLYPINSDINDKIGVLV
                     EHRKLNATNRYLRYGIQKISGKCYFFDVRNSVISKTKESAYNTFICPYCRERMFIVSA
                     FERQ"
     gene            complement(4453..4905)
                     /locus_tag="KRH39_003152"
     CDS             complement(4453..4905)
                     /locus_tag="KRH39_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513035.1"
                     /translation="MKLSRKNITMQVLSKFIENNLEKIENVETKANQIVFRTIDGDKK
                     IKIKTSKNYSEGEDPNTYLWTSFSSEELEEDYDYYTIICNEDIEKAVVFTKEELKQHY
                     VLETKKKKNREIDFELYPHFIGRRCIDARVHLYKEPIDITKYVNNYIF"
     gene            complement(4937..6304)
                     /locus_tag="KRH39_003153"
     CDS             complement(4937..6304)
                     /locus_tag="KRH39_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBG8513036.1"
                     /translation="MRYRYRDDEEMKDSGVEWIGKIPKDWEVKRIKHLFELKKDKSDE
                     ENPTVLSLTQKGLKIRDVSNNEGQLASTYVGYTKIEKNDFILNPMDLISGYTDKAEIE
                     GVISPAYTTFRSKNKVNINHDYYKRYFQMHYHHNFLFPWGEGVSFEHRWTLKNEVFLN
                     LPVITNRLEEQEKIANFLDEKTSQFEFIISKKEELIKKLEEAKKSLISEVVTGKVKVV
                     KTDDGYKLVKRSSEEMKDSGIEWLGEIPKDWEVKNFKYMFTLNKGLSITKADLKESGI
                     PCVNYGEIHSKYRFELKPSKHKLKYVDESYLKSNSISLLKYGDFVFCDTSEDIEGCGN
                     FTYLNENNKVFAGYHTIIARTLEQVNYRYMSYLFDSNDWRIQIRTKVSGVKVFSITQS
                     ILKGTKVILPDLLEQRNIAQYLDSKCKGIDSIIDKTKLQIDKLKEAKQSLISEAVTGK
                     IEILD"
     gene            complement(6304..8331)
                     /locus_tag="KRH39_003154"
     CDS             complement(6304..8331)
                     /locus_tag="KRH39_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBG8513037.1"
                     /translation="MSIDNNFNHIAFLWNIAESLRGTYKEEDYRKVMLPLIVIRRFDC
                     LLDDYDREIVKSVYKEYDFLPEEEKDELVIVDLKENHNIDLQFYNVSDFTWKKLLDDS
                     ENIKSNFEEYLNGFSNSVKEIIGKFKFKDEIAQLDKKDKLYAVLSKMYEVDLHINSVS
                     NNEMGYIYEEMLRRFTENSAAGEQYTPREVIRLCMEMLFMGKENFLTEEGKVISIADF
                     CCGTGGMLSIAEDYVEKVNPSAIVNVYGQELLDESFAICQADMIMKGQNPDNIRLGNT
                     LTQDRFSGEKIRFLISNPPFGVTWKDEEKKVKEEADLGFDGRFGAGTPRVSDGSLLFL
                     QNMISKMYDDEEGSRIAIIFNGSPLFTGDAGSGESNIRKWIIEKDLLEGIIALPTDMF
                     YNTGIATYIWVLTNKKEDKRKGKIQLVNASEYYQLMRKSLGNKRKEISLEQIEEIKEI
                     YERFEESENSKIFDNEGFGYRKVTIERPLKLSFRVNEEAIENVKNTTQFINLSVSKKK
                     DEEVKVKEEAEGRVKQDKLLKLLESFDSEFEYMKRDKFIKDLKSKSKLYDVALSAGLI
                     KAIVNAIGVRNEDAVVCKDAKGNIESDSSLKDTESIALKEDVYEYFEKEVKPHVEDAY
                     IDESSIDNIGYEIPFTRYFYKYEKLKSFDDIMKEVESLESEIALEIRKVLG"
     gene            complement(8447..8796)
                     /gene="ssrA"
                     /locus_tag="KRH39_003155"
     tmRNA           complement(8447..8796)
                     /gene="ssrA"
                     /locus_tag="KRH39_003155"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8954..9409)
                     /gene="smpB"
                     /locus_tag="KRH39_003156"
     CDS             complement(8954..9409)
                     /gene="smpB"
                     /locus_tag="KRH39_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBG8513038.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT         2845 a         1754 c          944 g         4147 t
ORIGIN      
        1 ttaagtcaca ctttaattca tatatgaata aatttttagg ttttattatt ttatctgtca
       61 tattgtactc ctttttattt atctatttgc ttctgcatat attttttcag ccaaataatg
      121 aatcatataa ttttgaagat tattattagt taatatttct ttaaccaatc tatcattcat
      181 attcttagtc ttaactatac ctctcttaaa ataactcttt ccaaaagtac taaccaaatc
      241 ttcataactg ttagctttag ctatatccat aatactctta tcatttttaa acatctcttc
      301 atattgatct attataatcc tatcttcctc tgtaagtcct actccaaaga catcatttat
      361 tttttggatt atatcttcta aatattcttc atcagcttca cttacacgag aagtagcagt
      421 ttcagatata ccaactaatt caccttcacc attatataat gatatatcac tacctttttt
      481 accatcttca cctaactttt gtaacttata ataatctaaa cttaaaatat catctatatc
      541 tattctttct ttaccctttt taggtagctt ccttaaaagt gctactaagt aaacctgtaa
      601 ccttaaaaga tttaagttct ttatagggta aatttggatt aagaaattat aaaaagtaat
      661 atacttttga cattttttct taaactcatc tgctgacttt tcatcttctt catctaaata
      721 acaatatctt tcaactgcct tatcaattaa actattcatc ttagcatctt gtgtatttgt
      781 tcttttatcc ttatagaata atactgcaaa gtcatttact tcctcatcag tatatatacc
      841 atattcatgt aactgatacc aaagatcatt tactacatta ggctctgtaa ctttatctat
      901 gtttgtcatt tcaaagtatg gtttatatgc ttcttgaata tcctctacac tattttggaa
      961 atccaagata aatgtatgct tattaggata tgttctatta agtcttgata aagtctgaac
     1021 agtttttaca ccatctaact tcttatcaac atacatagca cataactttg gttggtcaaa
     1081 accagtctga tacttttctg caactataag taatctaaag tcactttttt caaaaatact
     1141 tggtaaattt gaatcattaa tatctggtgc tacttcctta ttcataccaa cttctgtata
     1201 ttcaatatcc tcatctttta cagtatctga aaaagcaact aaggatttca tattgtaccc
     1261 tttttctttt atatattgat ctattgctaa tttatactta acagcatgta atctacttgc
     1321 tgtaactatc attcccttag ctctgccttc taaccacata gacctattat ccacaaaatc
     1381 gtctactata gtttcaactt tctgacgtat gttgtgttca tttaaactaa taaatttgtt
     1441 tatagctctt ttagcttctc ctttatcaaa ttcgggatta tcctctattt ttttacctac
     1501 cttataatat acactaactg gcataaagtt ctttaatacg tctaatatga atccttcttc
     1561 tatagcttgc ttcatagaat ataaatgaaa ctcatgaggt agtccatctt ctcctattct
     1621 accaaatact tgcatagtct tattcttagg agtagcagta aatgcaaaga aacttatatg
     1681 atctaaatta gttctctttt ctataaggtc attcatcttg tctatactag atttttcatt
     1741 cttttcattt tcagcatcaa ttatcttagc ttcttctaat gtttttcctg ctaaagtttc
     1801 ttttaatgca ttcatattct caccagatgt agaagaatga gcttcatcta ttattactgc
     1861 ataacgtctt ttttcaagtt gttctatctt aggtaatgca tattggaact tttgtatagt
     1921 tgtaataata atctttgctc tgtttacaat agcatcagct aaatctgatg aatttttatc
     1981 atcatctatc ttaacaacta atccgttttt gtgttctaat tggtatatag catcttgaag
     2041 ttgcttatca agtacctttc tatctgtaac tactattaca gaatcaaata tagcattgtt
     2101 atcttcatca tgaaggcttg ataatctatg agcagtccat gctatagtat tagtctttcc
     2161 actacctgca ctatgctgga taagataatt tttacctgct ttatttactt ctaaatccgc
     2221 tactatcttt cttactgcat ctagctgatg aaatctagga aatatagctc ttttactttt
     2281 cttatcatct tcttggataa agtaaaatct ctttataata tcaagtaaac tatctttagt
     2341 aagtatttct tcccaaagat aagaagtttt ttcttttcct tctactacag gatttccctt
     2401 acccatattg ttacctttat taaaaggtaa gaagaatgtt ttatctcctt ttaactctgt
     2461 tgtcatatat acttcgtcag tatctactgc aaaatataca aatactcttt gattaaatcc
     2521 aaataaagtt tcttttggat ttctatcctg tttgaattgt tttatagcat tatacacatt
     2581 ttgattagtg aattggtttt taagttccat tactataaca ggaaatccgt tcaaagataa
     2641 tactatatct aaagtattgt tgtgctttga actatatttt aattgtcttg ttattttgaa
     2701 tatattttgc ttataatttt cgtaagctgt tttgttagta gttagatttg gtttgttgta
     2761 gaataattgg aattttccgt tcatcattat atcttctaca ccatttctaa gtacacttat
     2821 aagccccctt cttttgatgt ttgagttaat taaatctata aaggcttttt tgtaattact
     2881 tcccctgttg tatttgaact catctagtaa ttctttttga gtaatttcta aaaatctaaa
     2941 taaatcttct acgaataagc aattttcaag atcaaattct tgtgaatgac cttgttcgta
     3001 tccaattgaa actaaatgat tttcaataga ctcttctaaa tgtttctctt ttggtaacat
     3061 tgctattccc ccatttctat attgttaatc tatatattat aaagcttata ttaatcatta
     3121 actacttacg ttttttacca gccacaccaa ctttatagcc atcatctctt aaatattttc
     3181 ctattattgc aggtatactt cgataagctc ttttgctatt tttcataatt actgcatata
     3241 taattcttgc ttctttattt gctttaacat caaaatatac taattctttt ttaggttcac
     3301 gtccattacg cttatcgctc cttttctgct tatctgtcct tttatctaca tattctttga
     3361 tcaaaatatt attttcattt aaattattta agtttttttt atatcataat taactttatc
     3421 atcaaattct ttatattctt tttcacgtat tttttccttt gaattatttt cttttacatt
     3481 attttcttta tttaaatttt tatgtttaga tattatatta tttgacttat ctataatctt
     3541 ttgagattca cttatttttt gctttctagc tttatctaaa atagggtcat ataaataaga
     3601 tattattaaa tccttagaaa gtctatatcc atctaaatgc atatccataa tttcagtttt
     3661 ccttatttct attacggttt tacctaaaac tttccattca tcaaaataat ctgctgtatt
     3721 tacagattta taaactgata tctcacaata tatttctcta ttgttatcag tgtaaagtat
     3781 tatatccggt gtatattttt tattattttt ttgacttgta ttataaattg gaatagcatt
     3841 tacaatatta actctttctt tttgtatatc tgctcttata gtattggtat gtagattttt
     3901 atataattta tatgaactct gtatacttaa tttaaagcct tcttgtgcac ttttacatac
     3961 ataatcttgt atctctctat gtataaattc ttctaattca taataattct gtcttagttt
     4021 catactttta gaatctgttc taaatataca ttatttattt gtctttttat aatgtttcaa
     4081 gtgaattggt actatgcttc attgtctttc aaatgctgat actataaaca ttctttctct
     4141 gcaataagga catataaaag tattatatgc actctccttt gtttttgata ttacactatt
     4201 tcttacatca aaaaaataac acttccccga aattttttgt ataccatatc ttaaatacct
     4261 attagtagca tttagttttc tatgttctac aagaactcca attttatcat ttatatcact
     4321 atttatagga taaagtctcg tatcttcttc tattttaaat ccatctttgt atcttatatt
     4381 taatgttttc atatcaatat aatctcctac ataaagcaat gtaaaatata attttattga
     4441 ttatatatat tactaaaata tatagttatt tacatactta gttatatcta ttggttcttt
     4501 atataaatga actctagcat ctatacatct tcttcctata aaatgaggat ataattcaaa
     4561 atcaatttct ctatttttct ttttcttagt ttctagtaca tagtgttgtt ttaattcttc
     4621 ttttgtaaat accacagctt tttctatatc ttcattgcat attatagtat aataatcata
     4681 atcttcttct aattcttcac tagaaaaact tgtccataaa tatgtattag gatcttctcc
     4741 ttcgctataa tttttacttg tttttatctt tatttttttg tcaccatcta tagttctaaa
     4801 aactatttga tttgcttttg tttctacgtt ttctattttt tctaagttat tttctataaa
     4861 ttttgataaa acttgcattg ttatattttt tctacttaat ttcatatttt tcacctctgt
     4921 tcaaatatac taagagttag tctagtattt ctatctttcc tgtcactgct tcacttatta
     4981 aactttgttt agcttctttt agtttatcta tttgtaattt agttttatct ataatagaat
     5041 ctatcccttt acatttacta tctaaatact gagctatatt cctttgttct aataaatctg
     5101 gtagtattac ttttgtaccc tttaatattg attgagttat actaaaaact ttaacccctg
     5161 atacctttgt gcgtatttgt attctccaat catttgaatc aaatagatac gacatatatc
     5221 tatagtttac ttgttctaaa gttctagcta ttatagtatg gtatccagca aacaccttat
     5281 tattttcatt taaatatgta aaattaccac acccttctat atcttctgat gtatcacaaa
     5341 aaacaaaatc tccgtacttt aataatgaaa tagaattcga ttttaaatat gattcatcta
     5401 cgtactttaa tttatgttta ctaggcttaa gctcaaacct atattttgag tgtatctctc
     5461 cataatttac acacggtatt ccactttctt ttaagtctgc ctttgttatt gataaacctt
     5521 tatttaaagt aaacatatac ttaaagttct tcacttccca atccttaggt atttcaccta
     5581 accattcaat tccactatcc ttcatttctt cactacttct cttcacaagc ttatatccat
     5641 catcagtctt aacaaccttc accttaccag taacaacctc acttataagg ctctttttag
     5701 cttcttctaa cttttttatt aattcttctt tcttagatat aataaattca aactgtgaag
     5761 tcttttcatc aaggaaatta gctatttttt cttgttcttc taacctattt gttattactg
     5821 gaagatttaa aaatacttca ttttttaatg tccatctatg ttcgaatgat accccctctc
     5881 cccacgggaa taagaaatta tgatgataat gcatttgaaa atatctttta taataatcgt
     5941 gattaatatt tactttattt ttacttctaa aggtagtata tgccggacta attacacctt
     6001 caatttctgc tttatcagta tatcctgata ttaaatccat tggattaagt ataaaatcat
     6061 ttttttcaat cttagtgtat cctacatatg ttgatgctaa ttgtccctca ttattactta
     6121 catctcttat ttttaatcct ttttgagtta atgataatac cgttggattt tcttcatcag
     6181 atttatcttt ctttaattca aataaatgtt ttattctttt tacttcccaa tccttaggaa
     6241 tcttacctat ccactcaact ccactatctt tcatttcctc atcatctcta tatctatatc
     6301 tcattaccct agcaccttcc ttatctccag tgctatctca ctttctaaac tttcaacttc
     6361 cttcataata tcatcaaagc tttttaactt ttcatactta taaaaatatc tagtaaaagg
     6421 tatctcatat cctatattat ctatactact ttcatctata taagcatctt caacatgagg
     6481 ttttacctct ttttcaaaat actcatacac atcttcttta agtgctatac tttcagtatc
     6541 ttttaaacta ctatcactct ctatattccc tttagcatct ttgcaaacta ctgcgtcttc
     6601 atttcttact cctatagcat taactatagc tttaattagt cctgctgata aagctacatc
     6661 atataactta gacttagatt ttaaatcttt aataaactta tccctcttca tatactcaaa
     6721 ttcactatca aaactttcta aaagttttaa taacttatct tgttttactc ttccttctgc
     6781 ttcttcctta actttaactt cttcatcttt tttcttagaa actgaaagat ttataaattg
     6841 agtagtattt ttaacatttt ctatagcttc ttcatttact ctaaagctca gttttaaagg
     6901 tctttctatt gttacttttc tatatccaaa gccttcattg tcaaatattt tactattttc
     6961 actttcttca aatctttcat atatttcttt tatttcttct atttgttcaa gacttatttc
     7021 ttttctttta tttcctaaac tttttctcat taattgataa tattcactag catttacaag
     7081 ttgtatctta ccttttcttt tatcctcttt tttattagta agtacccata tatatgtagc
     7141 tattcctgtg ttatagaaca tatcagtagg tagtgctatt ataccctcta gtaaatcctt
     7201 ttctattatc cattttctaa tgttagattc accacttcct gcatctcctg taaataatgg
     7261 tgatccattg aatattatag ctattctact accttcttca tcatcataca ttttgcttat
     7321 catattttgt aagaatagta acgacccatc acttactctt ggtgttcctg ccccaaatct
     7381 tccatcaaat cctaagtcag cttcttcttt tactttcttc tcttcatctt tccatgtgac
     7441 accaaatggt ggatttgata ttaaaaatct tatcttttct ccactaaatc tatcttgcgt
     7501 aagagtattt cctagtctta tattatctgg attttgtcct ttcattatca tatctgcctg
     7561 acatattgca aatgattcat ctagtaattc ttgtccatat acatttacta tggcacttgg
     7621 atttactttt tccacatagt cttcagctat tgaaagcatc ccaccagtac cacagcaaaa
     7681 atctgctata gatattactt ttccttcttc tgtaaggaag ttttctttgc ccataaataa
     7741 catttccata caaagtctta taacttctct tggagtatat tgttctcctg ctgctgagtt
     7801 ttctgtaaat cttcttagca tttcttcgta tatgtatccc atttcgttgt ttgatacaga
     7861 gtttatatgt aggtctactt catacatttt gctaaggact gcatatagct tgtctttttt
     7921 atctaattga gctatttcat ctttgaattt aaatttccct attatttctt ttacactgtt
     7981 tgagaatcca tttaggtatt cttcaaagtt cgattttata ttttcactat catctaacag
     8041 ttttttccat gtaaaatcac tcacattgta gaactgtaag tctatattat ggttttcttt
     8101 tagatccact attacaagtt cgtctttttc ttcttctggt aagaaatcat attctttgta
     8161 tactgatttc actatttctc tatcataatc atctaataga caatcaaatc ttcttattac
     8221 tattagtggt aacattactt ttctgtagtc ttcttctttg tatgtacccc ttaaactttc
     8281 tgctatattc cataaaaaag caatatgatt aaaattatta tctatactca cttatactat
     8341 ctccttttaa ttaaatttta aattagttta tattataatt atatcatttc atggcacaaa
     8401 aaaaacatat tgacaaaact atcaatatgt ttttcgtgga tttcaatggt ggagacgagg
     8461 ggagtcgaac ccctgtccgc aaatcttttc cttgctgcat ctacatgctt agccaacttc
     8521 actaatatct cacctcacat agcgccaagt ggcaggctct atgcttagcc agtcccaatt
     8581 aatctacttc aggtcgagac acccctaaag tagttccctg cataatgacg tccagtctta
     8641 gtcggcagga ggactaagag ggacgagcag caactaggct gctatcgcga aattatcgtc
     8701 tgcgtttatt ttaaagttcc ccagttttta cgtgacccag agtaacacga catgcagcct
     8761 caagttccaa acccacgtcg aaacctttac gcccccttga gtagtttata gtccagtaga
     8821 cttttcgcaa actttatcac catttttatt aaatttttaa tgtatatata atataatact
     8881 tttaagaata aaaatcaatg ttatgttcta gataataaag aattttattc taaaaaatac
     8941 agttttatct agtttaatac ttacctgtca tttctctttc tatacgtctt tgtgcatctt
     9001 tcttagccat atcttgacgt ttatcatata gttttttacc ttttgctaag gcaatctcta
     9061 ctttaacttt accgtttttt agatacaaac taagtggtat aagtgaatac ccctttatag
     9121 tagttgctcc aataagcttt cttatttcat gcttatgaag taatagtttt ctaactctta
     9181 aagaatcaac attgaatata tttccttgct catatggact tatatgaact tgttttaaaa
     9241 atacttcgct attatctaca gatgcaaatc catcacttag gttaactttg ccttgtctta
     9301 ttgattttac ttctgttcct tttaattcaa ttccacattc ataagtttct tctataaaat
     9361 attcatgtct tgctcttttg tttgttgcta atattttttt accagccata taaatctcac
     9421 cttctttgct agaaatttgt tccataaatg ttagaaattt actaaatatt taatgttgcc
     9481 aaattatttg actaacttgt atgataacat atttaggcat tttagtcaat atttcactgg
     9541 tatattaagt tagttaggct atatataatt aagtaatata aatgtataaa aatagctaaa
     9601 aaactacttc gaatatactt aatactaaac taactctaac cagtaatact aatttataaa
     9661 tttcaaaaca aaaatattac atattataaa
//
LOCUS       DAEORZ010000146         9687 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEORZ010000146 DAEORZ010000000
VERSION     DAEORZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.149"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            406..1308
                     /locus_tag="KRH39_003157"
     CDS             406..1308
                     /locus_tag="KRH39_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513039.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLNIS
                     LKKNKCKASTKNKSEKRGVVDVTN"
     gene            1295..2050
                     /locus_tag="KRH39_003158"
     CDS             1295..2050
                     /locus_tag="KRH39_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8513040.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            2181..3203
                     /locus_tag="KRH39_003159"
     CDS             2181..3203
                     /locus_tag="KRH39_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8513041.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(3367..3924)
                     /locus_tag="KRH39_003160"
     CDS             complement(3367..3924)
                     /locus_tag="KRH39_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG8513042.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(3930..5006)
                     /locus_tag="KRH39_003161"
     CDS             complement(3930..5006)
                     /locus_tag="KRH39_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBG8513043.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(5007..5690)
                     /gene="modB"
                     /locus_tag="KRH39_003162"
     CDS             complement(5007..5690)
                     /gene="modB"
                     /locus_tag="KRH39_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBG8513044.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(5839..6651)
                     /gene="modA"
                     /locus_tag="KRH39_003163"
     CDS             complement(5839..6651)
                     /gene="modA"
                     /locus_tag="KRH39_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8513045.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            6844..7773
                     /locus_tag="KRH39_003164"
     CDS             6844..7773
                     /locus_tag="KRH39_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8513046.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(7934..8383)
                     /locus_tag="KRH39_003165"
     CDS             complement(7934..8383)
                     /locus_tag="KRH39_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513047.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(8586..9566)
                     /locus_tag="KRH39_003166"
     CDS             complement(8586..9566)
                     /locus_tag="KRH39_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8513048.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         3341 a         1553 c         1205 g         3588 t
ORIGIN      
        1 ttactgtatt aaactaatta ctatacaaaa cagaaaacaa aattttcaaa attattaaat
       61 taaaaggaga ggtaaatatg aagactttat taaacaaaca agtacgattt tttagtagcc
      121 aatttagtta gggtttgtac ctaagttaag acaaattgtc ataacataga tacaagcccc
      181 ttaggattat gatttttaag gattgtatct atgttggcta tagtaattgt tgttaaagtt
      241 tttattttat catcataata tagagccaca tggattatac tgtgtggctt attcgaattt
      301 ttgccataca gttatatata ctgtgtggtt tttttatatc aaaatcattt ttaaaaacca
      361 aattttaaaa accatttaaa atattttcaa aggggtgttt tatttatgtc aggtattata
      421 tctgtaatga cgcaaagttt aattttatcg attatggcac tgggggttta tataacttac
      481 aaaatcttag attttccaga tatgtctgct gatggtagct atacaatggg tgcttctatt
      541 gtagcatttt ctcttactaa tggtatttct cctgtagtag caactttaat ggctatctta
      601 tgtggttgta ctgctggtct tgttacaggg attcttcaca ttaagtttaa gatatctaat
      661 ttactttctg gtattttagt tatgggaatg ctatactcta tcaatttaag aataatggga
      721 aaatcaaata ttcctctatt tagctttaaa cacttattca atggggaaat atctcctata
      781 gttttagcct tagcttttgt attcatctgt aaagtccttt tagatttatt cctaaaaaca
      841 ggtcttggat acactttaaa aggtgttggt gataactctc aaatgattaa atccctaggt
      901 ataaatatcg gttctataaa aatactagga cttatgattt caaacggact tatagcttta
      961 tctggtagtc ttatggctca gttcttggga ttctcagatg taaatatggg tataggaact
     1021 ttagttcttg gaattgcctc tatcataatt ggtattacac tatttaagaa atttactttt
     1081 atcaaagata ctactgcaat aattgttggc tcttttatat accaattcac tatatacttt
     1141 gcaatgagtt taggaatgtt atcaactgac ttaaaactaa taactgccat agttataata
     1201 gctttcctag ctactggtaa cttaaatatt tcactaaaaa aaaacaaatg caaagctagt
     1261 accaaaaata aatcagaaaa aagaggtgtt gtagatgtta caaattaaaa atctttcaaa
     1321 gagtttccct aatccatatg gtgaacctaa tactatcttt gaaaatttat caatcgatat
     1381 agaagatggt gaatttgtaa gtatcatagg tagtaatgga actggtaaat ctactttatt
     1441 aaatataata tccgggctta taaaagaatc ttctggtcaa gtattattaa ataaacataa
     1501 cttgtctaat ttagctgaac acagaagaac tcaaatagta agtagagttt ttcaagaccc
     1561 aagtcttgga acttgccctt ctatgactgt tagagaaaac ttgtctttag ctcttaacaa
     1621 aggaaaacta ttaaatctaa gaaaatgtct tcgttacaaa agagatttct tagaaaattt
     1681 attggaaggt gtatctcttg atttaaaaaa ataccttgat gttcaagtaa aatttttatc
     1741 aggaggtcaa agacaatcat tatcactaat tatgtcgtgc ttaacaaatc catcagtttt
     1801 attacttgat gaacatacag cagcacttga cccaaaaaca tcaaatgaag taattgaatt
     1861 gactaataag attgtaaggg aaaaaaatat aactacactt atggttactc acaacttaaa
     1921 acacgctctt cattatggtg atagattgat aatgcttcat aagggtgaag ttgtattaga
     1981 tgtaaaaggt aaagaaaaag aacaattaac agttgaagaa atcttagaaa aatttgaata
     2041 tgcagtttaa aataatatat ctattgtatt aaactcggct ttaaaataaa gttaaaatta
     2101 caataattat ggttgctttg taataattca tatgaatata tgaacatata taacaataaa
     2161 tttttgaggg ggatataaaa atgattaata aaaaaagatt ggcaagtttg attttagctg
     2221 gtgctcttag tatatctatg cttacagggt gttcacaagg aggagactct ggtaattcaa
     2281 aacaagaaag taattctaaa ggtaaagaag ttaaaaaaat tggtataact caactggttg
     2341 aacatccagc attagatgcc actaggacag gttttgtaaa agcattagaa aagaacggat
     2401 ttaaagatgg agaaaatatt gatatagact tccaaaatgc tcaaaatgac atgcctacta
     2461 cacaaagtat tgcgagtaaa tttgcatctg ataagaagga cttaatattt gctatatcta
     2521 ctccatcagc tcaggcagca tttaatgcta ctaaagatat acctatactt attactgctg
     2581 tgagtgaccc tgttgctgct ggattagtta aaactcttga aaagccaggc acaaatgtat
     2641 ctggaacatc agattttgtt tctgtagaca aaggtttaga acttcttaaa atatttgctc
     2701 ctaaggcaaa aactatagga gttatgtaca atactagtga agtaaattcc aaagttcaag
     2761 ttgatgcttt aaaagaatat gcatctaaaa atggatttaa agttgttgaa aaaggtataa
     2821 ctacttcaaa tgaagttaac caaggtattt ctagtttagt tggcaaaata gacgtattat
     2881 atgtccctac agataactta gtggcttctt ctatgcctat agtctctaaa atagctactg
     2941 agaataaaat acctgttata gcagctgaat ctggtccagt tgaaaaaggt gctcttgctt
     3001 gtcaaggtat caactacgaa aaactaggtt acaaaacagg tgaaatggca gttaaaattt
     3061 taaatggcga atcagtatct gatatgcctg tggctacatc agatgataca gatatcattg
     3121 ttaatgaaga tatattaaaa gctttaggta tggaaaaacc ttctaatgaa aatatttctt
     3181 atgtaaagac taaacaagaa tagaaagata ttttctatat acgattaata tgcctgtttc
     3241 tataaattaa tttatagaaa caggcatata tttttatttt aatgtattat ctatttaaag
     3301 attatatgta aatgctctta atttgtttgt ataaatttta cttgtataaa ctttatacaa
     3361 tcatatttat tcttctattt tctttattac tctacatgga tttccaacag ctacagtatt
     3421 tggtggtata tcttttgtga ctacacttcc tgcacctatt actacattat cacctatagt
     3481 aattcctggt gttatgatta ctcccccacc tatccataca ttatcaccta tttttatagg
     3541 agaaccatac tctatgcctg aatttctaag ttgtgcatca attggatgat aggctgtata
     3601 gatttgaacg tttggagcta acattacatt gtcacctatt tcaattttac atacatctaa
     3661 aaaaatacaa tcataatttg caaagaaatt ttcgcctaca tgtatgttat atccataatc
     3721 acacctaata ttttgttcta catttatttg cttgccaact gaaccaaata gttgtcttaa
     3781 aatatcttct cttttttcat attcatcctc taaagtatta ttaaacaatc tagttaattt
     3841 tttacaatac tctctttctt ttactaacaa ttcatcattt gcatagtatc ctttcccaga
     3901 caacattttt tctttttcag tcatatagtt tactcctttt tgttataatt gtatctaaag
     3961 caaaataaat attcctgtgg aaaatctaaa tatattctgt cacctttctt aaatctcatt
     4021 ttactttttt ccaattctat ttgaataggt acacattcgt tagataaatc agtttttcta
     4081 acatatatag tatatgtaaa aggattttct acaatgttaa tcaattcgaa acataacttt
     4141 tcatttaaat cttcattaga atccaagacc ttaatatgat gctccctgat tcccacatat
     4201 tctatattat cacctatttc tctatttaat gtcaactcgc atccccagtc tgtagcataa
     4261 atagtatttt tatccagtct atttaatttg gatatatttt tacaccctgt tatcttagct
     4321 tctataaggt actttggaga ttcaaagagc tcttttttag gtctctttgg aagaccttct
     4381 cctttgttgt acactataat atcatcacaa actcggtaag cttcttcaat atcatgagta
     4441 acaaatagta tatctccctt gtaatctttt aacatattca taagttcttt ttccatattg
     4501 cttcttaaat gatggtccaa tgctgagaaa ggttcatcta aaagtaatat atctggagat
     4561 gttgccaatg ctctagctag tgctaccctt tgttgttgac caccagataa ttgccaaggg
     4621 tatctcccct caaacccatt taatttaagt atatctaagt accttgctac tatttccttt
     4681 ttttcactct tacttagttt taaaagccca agttctatat tttgacttac agtcatatgt
     4741 ggaaacaaag catagttttg aaataaaaat cctacctttc tatcttgtgt tgataaattt
     4801 atcttctttt cagaatcaaa tagaactcta tcatttaata caattttacc tcttgttggt
     4861 ttctcaagcc ctgctataca ttttaatgtc atactttttc cagaaccaga ttctcccaaa
     4921 aaccccagag tacctttttc ttgtttaatt tctaccttta gtttaaaaga ggataaatct
     4981 ttttcaatat ccacgtataa gctcaactat ccacacctct ttcctataat tttctgctga
     5041 aattccgacc aataatttag taataaaatc attgctatag atattgctac aataatcata
     5101 acccacatca tagctttatt catatctccg ccttcaacag caaaaaatat agctattggc
     5161 atagtttgag tttttccagg tatatttcca gctatcataa gtgtagcacc aaattctcca
     5221 agagctctgg caaacgacag caccaatcca ccaattattc caggccatgc taatggaact
     5281 gcaatcttaa aaaatacttt ccattcatta agacctaatg ttctagcagc tgacaacata
     5341 ttattatcaa tctgttcaaa agctgacctt gaagtcctat acatcatagg aaaagacacc
     5401 actactgctg aaattacagt tgctgtccaa ctaaatatta aagtcctatc gaaatttaat
     5461 aaaaactttc ctataaatcc attcttccca catagtaata ataaaaagaa acctacaact
     5521 gttggtggca aaattagtgg aagtgtaaat agtccatcta taagaccctt ccacttacct
     5581 ctatagttag ccataatata tgacacaaat attcctatta caaaagttat tattgttgac
     5641 aaaatagatg tcttaagtga tatccataag ggtgaccaat ctgttcccat ctttccctcc
     5701 ttaattttaa atggaaatag tgaaactaat aactctatta atttcactat ttccatttta
     5761 ttataataca caattatttt caattttcat ttatttatat ataatttata cttaattaca
     5821 cataatttat ttctaagttt attctacttt tttatatcca aattcttcaa aaatcttttg
     5881 tcctgactca cttaataaaa attcttcaaa tttcttagca gcatctacgt ttttactagc
     5941 tttaattact gaaactggat atgtaatagg tgaatgagtt ttttcgtctg ttttttcaac
     6001 tacttttatc ttatcattat tgacagtatc actgaagtaa acgaatccaa catcagcatt
     6061 tcctgattgc acccatgcta aaacttcttt tacatctttt gcaaatacta gcttatcttt
     6121 taacttatct tttaaattta aattagttaa cacctcatct gcatacttac cagctggaac
     6181 actttcagct tcaccaacag caattttctt cactttatct gtagtcaaat ctttcatacc
     6241 tgatacacta ctgtcagcag aagaaataag aactaaatca ttttttacta aatctttcat
     6301 agtatcagaa actaataact tctcttcatc caaagctttc atttgttttt gacctgctga
     6361 aataaataaa tcacaaggtg ctccttgttc tatttgttgt tgtaatgagc ctgatgctcc
     6421 atagttaact gtaagtttta catttgaatc aactttttta tattcttctt caatctttgc
     6481 cattgcttct tttaaacttg cagcagccga tatatttaac tctactgaat cactactttt
     6541 agttgttttt tcttgttttc catcatcttt tttgtcttca ttactattgc acccaactgt
     6601 ccccaaagtt aaacatgcaa ctaatcctaa tattcccaat atttttttca taacaatctc
     6661 cctttttgta aatttgatac acattttaac atgttttatt atgttttatc atgttttgtt
     6721 atatatagtt taattatata tacatgataa taaattttca agtaatttat ttacactact
     6781 tttaaatatt ttgctacatt ttcctttttt taatgttaaa ataaccttat atggaggtgg
     6841 attatggact cattatcatc tttgacacct gtcgaggtag ctgaattatt aaagataaca
     6901 aaaaatactg tatatgaatt aatcaaaaga ggtgaacttc cttcttataa agtagggaaa
     6961 aaaataagag ttgatactaa agatgttgaa gaatatatca acagccaaaa aactggtaaa
     7021 gtaaaggata cacatttcaa caataaaaat atagatataa ataataattt caaaattatt
     7081 atttctggtc aagatgtaat attagacatt ttagctaggt ctgttgaaaa aaaattggat
     7141 ggaataagta catttcgctc ttatataggt agttataatg ctttgtatga gttatataat
     7201 ggcagagttt ctatagcatc atcccatcta tgggatttcg aaacggatga atataatagc
     7261 acttttgtta aaaagctatt gccaggaata ccatgtgtac ttataaatct agcttataga
     7321 atgcaaggtt tctatgttgc taaaggaaat cctaaagaga taacaacctg ggaggattta
     7381 acaaaaccaa gtataaccat tgtaaatagg gaaaaaggtt ctggaactag ggttttacta
     7441 gatggtaaac ttagacttct aaactttaat ggtaaacata taaaaggtta tgataatgaa
     7501 gaattatcac atcttggagt tgcaagtact gtttctcgtg gaatagcaga tgttggtttg
     7561 ggaaatgaaa aagctgcttt acaagtaaat aatatcgact ttattcctct tcacaaagag
     7621 cgctatgatt tagttattaa aaaagaagac ttacaaaatc cagtttacca aactataatt
     7681 aacataataa actctcctga gtttaaagct gaacttcaag gtcttggcgg atatgattta
     7741 actgatactg gaaaaactat agcaaaagtt taagattaaa atcgcaatct attttataag
     7801 atttataatt tttcacaaaa tttataactt aaacacgaaa taaattgcga ttttagtgtc
     7861 tcaaaaaatt tatgtctttc attttatcat ccaatacaaa atataatata attaattgtg
     7921 caatcatatt atattaatat atatttacat aatagtattt ttctttaatg tagattaaca
     7981 tatgatatgt tttaaacatc aatccccttg ctttcttata tatatgtggc aacacatatg
     8041 agtttaataa attatataca atgttgcttt cttcttgtat tttttcaacc aacaagttat
     8101 ttataaaatt acctttctca ttttcaataa tctcactcca atttatctta acttttaata
     8161 tatatcttcc cactaggtat acacactcta caataaataa taaaaataca atgcagtcag
     8221 tacctgttgt cttatataaa ttacccgata ctaatggctt tgcgaagaaa ttataaaaat
     8281 tcaaagtatc acccgtataa tcaaaaagta ttaatagcac tcctagaact ctcaatactt
     8341 taatattcaa catgttttta tctttattat ttttgttttt catatatact acctctcttt
     8401 tctttagatt tgattgtaat ctaatactaa ctcttttatt tgaggtataa atgaatttaa
     8461 ttggtagtta ttttcatttt tactagaaat taattcacat tactaaacaa aacatatatt
     8521 taaacataaa aatagcctat ctatctttta ataaatacaa aaataaataa gctactaata
     8581 ttcaattaac ttttatcact taaaacatta ctatctataa atttaaatat tttattgaag
     8641 tatactgtac tatcatgttc aaatccatca acatgaccaa taccttctat aagccatagg
     8701 tcttttttat catgtttaac taaatcatac attttcttgc tattttgata attacaaaca
     8761 gtatcttttg ttccatgtat aaacagtatg ggtacattag atttctcaac tgactcatat
     8821 ggttttacat cactgtacca aaatccagat ttaaacttat tatataaatc tccacaagtc
     8881 accacataat tgattaatat atctggtatt cgtttttcta gtactcccat tctaattgca
     8941 tctttcattt cagaataagg agaatccagt atataaaaat cgaccttatc atcttttgaa
     9001 tttatctctg aatgcatagc agctgtccct gcccccatgg aaaatccatg cacacctaat
     9061 ctcccttcag gaaatttatt ttttacaaat ttaactagac tatctaaatc atacctttca
     9121 taaagaccaa atgtgtaatt atctccacca ctattaccag tatttctttg attataaact
     9181 aatacattat aacctttatc taaatatcta tatgctacct ttaacatttc atagtaacta
     9241 cttccaattc catgaacaag tatcatagta tctcttgtct ttatatttga agttataaaa
     9301 agactttcaa gcttgtaatt attagaactt tgtatcatta attcagatgt cttgtagttt
     9361 tttaatttct ccagcggttt atcttttctc tcagaaaata tttttactat atcttcgttt
     9421 tttattaact gctctaaacc aaccgaacta ttatacacta atttgccaaa tatataagat
     9481 acaattaata ggattaatag aaacacaatt gatataatta taaatttatt tcttaaacta
     9541 atacaatttt taaaactatt tttcatattt atttaccata caattttcct atattatatt
     9601 ttctaagtaa tagcataatt gattatacta ttcttataat ttctattata cctacttatt
     9661 tattaaaaat acaaattata ttttatt
//
LOCUS       DAEORZ010000147         9664 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEORZ010000147 DAEORZ010000000
VERSION     DAEORZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.150"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            151..2217
                     /locus_tag="KRH39_003167"
     CDS             151..2217
                     /locus_tag="KRH39_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG8513049.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVIQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            2236..4191
                     /locus_tag="KRH39_003168"
     CDS             2236..4191
                     /locus_tag="KRH39_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG8513050.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKSGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     NEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKFTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLVIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            4207..5397
                     /locus_tag="KRH39_003169"
     CDS             4207..5397
                     /locus_tag="KRH39_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG8513051.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(6278..6461)
                     /locus_tag="KRH39_003170"
                     /pseudo
     CDS             complement(6278..6461)
                     /locus_tag="KRH39_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6578..6952
                     /locus_tag="KRH39_003171"
     CDS             6578..6952
                     /locus_tag="KRH39_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8513052.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            6954..7811
                     /locus_tag="KRH39_003172"
     CDS             6954..7811
                     /locus_tag="KRH39_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513053.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALSEDCITGFIGVNGAGKTT
                     TIRSILDVIKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            7814..8452
                     /locus_tag="KRH39_003173"
     CDS             7814..8452
                     /locus_tag="KRH39_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG8513054.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRSGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTMAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            9471..>9664
                     /locus_tag="KRH39_003174"
     CDS             9471..>9664
                     /locus_tag="KRH39_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8513055.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKN"
BASE COUNT         3518 a         1150 c         1701 g         3295 t
ORIGIN      
        1 aagtatgaat taagcatgaa ttaagcatgg attaaatata ctttaaatta caaatattac
       61 aaggaagcca gaaggcttcc taaaaaaata acctttataa aattttttat tttaaattta
      121 aaatttttta ttttaaattt aaaataaaaa atgaaagagg tggtagagag tttgaaaaaa
      181 attactaaag aaaggataga aaatactatg aaaaagctat ttacgagtga aacatttttg
      241 gaatatcaat ttataagtga ttgccaaata tcaccagatg gaggatatac agcatttatt
      301 gtaaaaaaag ctgatatcaa agagaatggt tacacaagcc aagtttatat attaaacaat
      361 aaaactggcg aattaaaaca aataacgtct ataaactctg ttggagcata tgcctgggaa
      421 gatgaaaata ctatcttgtt ccctgctttg cgaaatgaaa aagtaaaaga agctgtcaaa
      481 aacggtaagc aatgcatgag ttactatgct ttatctttaa atggtggaga agcagaagaa
      541 ctttttagac tccctatcaa aggtggtaaa ttgaatccta taggaaaagg tttgtatgca
      601 gttattgatt cttatgacaa tgaccgacct attgtagagg gattgccaga agaagaacaa
      661 cagaaaaaaa taaatgctta taacaaaaga cattatgaac atttcaaaga aattccttat
      721 gctgtcaatg gggagggata tattagcaga aaaagaaaac gcctatatgt atacgacagt
      781 aatataaatg agttgaaggc aattacagca cctatgttta atgttgtagg tatgaaaata
      841 agtgatggaa aaatcttata tatagggcaa gagtttaaag atgtaaaagg tttgaaaaat
      901 ggggtctatg tatttgatac aaacacaaac acaaatgtct gtatattaga aaaagataag
      961 tatattataa aaggttttga attatatcaa aatcaagtga tactaaacct aactgatgct
     1021 ttatcttatg gaaatggaga aaatggagat ttttatacta ttgatattga tacgaaagaa
     1081 atgaaacttc tttcagggca tcaacatcat tgtattggaa atacagtaac atctgatgtt
     1141 aaaatgggtg ctggtcagac aacaaaggta gatggagaat atatttatta cacttctaca
     1201 gtagatatgg actgcattat tgaaagaata catattccaa caggaaaaca ggaaaaagta
     1261 atacagacag gttcagttga ttttatagat gtaaaagatg gcaatattgt atgtgttggt
     1321 tgtttaggaa atggattacc agaagtttat acagtagaga atggaacatt gtgtaagaaa
     1381 acacatttaa atgaccatat tttagaggaa tataaaattt ctgtgccaga atacatagag
     1441 tcaaaaggaa gttctaaatg ggaaatacaa ggatacgtat ataaaccagt agattatgag
     1501 gtggggaaaa aatatccagc tgtattggcg attcatggag gaccaagatt gacatatgga
     1561 ccatatttta tgcatgagat acaggtattt acaagtgcag gttactttgt attcttctgt
     1621 aacccaaggg ggtctgaagg tcgtggcaat gcttttgcag atattaggaa acagtttgga
     1681 gatattgatt atatagattt tatggaattt acagatactg tacttgaaaa atatacagac
     1741 attgataaaa caaaattggc tgttgaaggt ggaagttatg gtggatttat gacgaattgg
     1801 ataattggtc atacaaaccg ttttgctgtt gcttgtgctc agcgttctat tgcaaactgg
     1861 agtggcatgg agggtacaac agacattggt tattatttct gcaaaggaca aacaggtgct
     1921 tctcatatgg aaaaccacga gcttcaatgg aaacagtcac ctcttgctta tgctgataaa
     1981 tgtgttacac cgactttatt tttacatggt gaaaaagact atagatgcta tatgcaagaa
     2041 gcattccaga tgttttcagc attgaaaatt catgggtgtc ctacaaaact ttgtctattt
     2101 gaaggagaaa accatgaact gtctcgttct ggtagaccaa aacaaaagct acaaagatta
     2161 gttgaaatgc tagattggtt ttcggtatat ataaaaaaag ataacatagg acagtagaga
     2221 aaggagaata taaaaatgga aaaaataaag attgaaagtt cagatatttt tgaatatata
     2281 ttccctcatg atatttcttg ttcacctgat ggaaagcata ttgcttatat tatatcaaat
     2341 atcaacgagg aaaaagactg ctatgaacat gacttatatg tgatggacat caaaacagaa
     2401 aaacaaatac atatgactca aacaaaagat gtaacatcat ttagttggat ttctaataca
     2461 gaactacttt ttacatctaa aagaaataag ccaaaatcag gtacaacaga tttttacact
     2521 atttctatag aaggaggaga agcaaaaaaa gcattttcta ttccaaaagc ttgtagtgta
     2581 cctgtatcac ttggaaataa attgtggtta ttgacaacaa aaaatccaac ggattctaaa
     2641 aaatcagaac cagatagggc agtagagggt gtagattatt ggacatttac agataaacct
     2701 tttataagag atggagaaaa tttttctcaa agaagacgtg taacattaga attatatcaa
     2761 gaaggtgaaa atataacaaa agccattaca ccaaaatttt gtgaagtagc aggtgtagac
     2821 gtttcttctg ataaaaacag aattttatat actggtcaga tttatgaaga ttgtgcaact
     2881 cctttttctg gactttggga gtatcatata gacagtggtg aaaccaaaga acttgtacca
     2941 caaggaaagt accaaataag tttggcaaaa tatattggca aagataaagt aatgttgcaa
     3001 gcttcaacat tagaccgttc tatcacacag aatcatgata tttttatact tgatttgagt
     3061 actaatgaaa taaatatgat tgctagtcca gatggtatgt atgctacatt actggatgta
     3121 gatgcagtat atggtggtgg acgtagtaat aaagttatag gagacaaatt tattggtgca
     3181 agaatttgca gaactatgac agaatttaat gagtttgata caaagacagg taatatacgt
     3241 atcatcacaa aagtagatgc ttttacatct tttgatattt acgacaacac catgtacaca
     3301 gtaatgttga aagattatga gttagcagaa atatatagca ttgatatgac tacaggtaca
     3361 atgaaaaaaa tgactgcttt ttctaaacca tatttagata ctcataaggt ttcattacct
     3421 gaaaaattta cgtttgtagc aaaaaataaa gaagaagttg atggttttgt tataccacct
     3481 atagatgcta aagaaggcga aaaatatcct gctgtattgt tcattcatgg tgggcctaaa
     3541 tgggcatatg gctatatgtt tacacacttg aagcagtgtg taacatcaaa gggaatgtat
     3601 gtgatttatt gtaaccctca tggtggtgac ggttatggtg aaaaattctt agaaatggta
     3661 gaaagatggg ggtatgttga ctatgagcat cttatggaat ttgtagatac ttgtattgaa
     3721 aaatatcctg ggattgatgc agaccgctta ggggttgcag ggggaagtta cggtggttat
     3781 atgacaaact ggataattgg ccatacagac cgtttcaaag cagcagtttc tcaaagaggt
     3841 attagtaact tgattacagc ttctttagtt attgactttg gagatagaat tatgaagcag
     3901 acttgtggag ataagacgcc ttggaatcat gaggaggttc tttggaatca ttcacctatt
     3961 aaatatgtga aaaatgttaa gacaccaaca ttgttcttac atagcgatag ggattataga
     4021 tgttttatgg gggatacttt ccaaatgttt acagcattaa aacagcttgg tgtagataca
     4081 gaaatgtatt tgtttcacgg agatacacat ggattgtcta gaaatggaag accatctaat
     4141 agaattgcac gagcaaatgc tattgtagat tggtttgaaa gatatttata ataagggggt
     4201 tattctatga gtgatatgtt attatggttt agtgtcgtat tagttttatt tgctattggg
     4261 gatttaattg caagcaaaac aaaagcaaaa gtatcagctg tatttgttac actactttta
     4321 tttttgattc tatttgtcac aaaagcaata ccagctgata ttattgaaaa agcaggtatg
     4381 accgcagcag ctagttggag tgttccaatg attatgttta gtatgggaac aatgttgaat
     4441 gttaaacaat ttatagacga atggagaaca gtattgacag catggcttgg aattgtagca
     4501 gttatagtct gtgtctcatt atgtattcct ttgtttggaa aatcaacagt attgacaagc
     4561 ataccagtta taaatggagc tttacctgct actactatta tgacacaagc agcactagaa
     4621 aaaggactga cacttgcagc agctacagca acagttgtat ttgcaattca gaagtttatt
     4681 ggtacaccaa tagcttcaag agcagcgtta caagaggcaa accgtctttt agtagaatat
     4741 catgaagcaa aatcaaaagg cattgattta gcaaatgttg atactgataa gaaagaagca
     4801 gagaatacag gcacaaaagt aaaacaagct ttctgtgaaa aatacgataa atattattct
     4861 acaaatgttt gtatattttt tatagcatta ttctcttatc tagggtatga actttcagag
     4921 attattcatg taaattattc tattgtatgt cttgttgttg gtgttattgt aacacgtatt
     4981 ggtattgtgc caaaagacat tttggaaaaa ggaaaaatca agggatttat caacatggtt
     5041 gtgtttgcag ctgttattcc ttctttagca aaagtttctt tgacagattt aattagtctt
     5101 tttgtaccaa ttgtgggtat gtttgcagct tctatcattg gtatattcct tatgatgaag
     5161 gtgttaccag gttggaaaat tatcggttct aaacctcttg cttttggagt agggttttgt
     5221 cagatgttgg ggttcccaac tacatattta atttcaaatg aagtgtgtaa tgctgttgga
     5281 gaaacggaag aagaaagagc atatttgatg tctaagatta tgcctaagct ggtagttggt
     5341 ggtatggctt gtatgatttc tattgtggta gctggtctta tggtaccaat gttgtagata
     5401 ggtttcatag ttaaaaaagt ggtgttaatt tataggagaa gctaaaaagt agtattaatt
     5461 tataaataaa gctagaaagt cagtattaat ttataaacga attttatatt agttaattta
     5521 ttataagaat attttctttt tagcattata atcttgattt tatcaagaaa cagatttttt
     5581 atttgctatt ctaatagtta cagtttattt ttattttcta aaacaaaact ttaacttgat
     5641 ttatctataa atagggtatt taataatatt tttggaaaat ctttagaaat aattaaaaag
     5701 aggagaaaaa atggagtgcc tcaaagtgaa cttttttagt tcataaggca ctcttttttg
     5761 tacgaaatcg aatatatttt caacgtttta aaagtgagaa agagacttat ctctagtttg
     5821 agatagccaa ttttaattat taaaattatt ttttgtttta tttatcaaag agtattcgtg
     5881 ttttagaagt tagtattttt tattttatat atacaaggtt ttccgtttct aatgttttct
     5941 tttttaaaat aattagggta ttcaattatg gtaaaaggtg gatggtattt tgcagtgata
     6001 ttatttaaag cacagtattg aatgttgaaa ttctcacatt ttagagtatt atcttttgta
     6061 atcaaaaaaa tcttctatac ttatagcaag gttatttctt caaatattcc atgcatcata
     6121 atatctaaat ttaaattttt ttaatataga gtttacctct agtctacact cttgaataac
     6181 tctttcctct atccaaattc ttataaattc atcccattta ttactatcta tttattacta
     6241 tctaaatttt ttagtatgtt tgtaattaga aatggattta ttcctttatg ttttttataa
     6301 atagtttata acttaattta tcatcaaaca aatcattcat acaggattac tttttctttt
     6361 agatattcaa ttcttacttt taataaatat ttaatacatt ctatcttatg tggttttaaa
     6421 aagtgtttat agctatcaac aatctctaat atttcattca tatttatttt gtcatagtaa
     6481 tattttacat cttttatatt gacaaaaaag tgctctaagt gtatagtgtg tatatgataa
     6541 catcacactg aaagtgataa aaagggggtg ataaaaaatg aaagtgctca tttctaacac
     6601 atcagataag ccattatacc aacaaattaa agagcaaatt aaagaagcaa tatttactga
     6661 agaactgaaa gaaggggatg caatgccatc cataagaaat tttgctaatg atttaaaagt
     6721 tagtattttg actattagac gagtgtatga agaacttgaa aatgaaggct ttcttacacg
     6781 ccaagttgga ataggaacat ttatttcaac tggaaattta gaaatactaa gagactcaaa
     6841 gagacgtata gttgaacaaa aaatggcaga tatgattaag gatgcaaaag cactaggaat
     6901 tacacagata gagttacaag aaatgatgga tattctatat caggaggatt aatatggaaa
     6961 atattttaga agtttataac ttaaataaga cttataccga tttttcatta tctaatataa
     7021 gctttgcatt atcagaagat tgtataaccg gatttatagg tgttaatgga gcaggaaaaa
     7081 caacaacaat ccgttcaata ttggatgtca taaagcgtga tagtggaaaa ataaaaatct
     7141 ttggtaaaga tttcgatgga aatgagaagg aattaaaaaa tcgaattgga gttgtctttg
     7201 atgattcata tttctatgag gagttgtcaa tggcagaaat gaaaagtatt attgcacctt
     7261 catatgataa ttggtgtgaa gaagattatc aacaaaatat gaagcgtttt aatcttaacc
     7321 caaaacaaaa gattgataca ctttcaaaag gaatgaaaat gaaatttgct ctagcattag
     7381 cattatcgca taaagctgag tttttaatta tggatgaacc tacaagtggt ttagacccac
     7441 taatccgtag tcagatactt gacatactaa cagaatatat gaagaaatgt ggtcgtggtg
     7501 ttttcttttc cactcatata acatctgatt tagacaaaat agcagatatg attgttatga
     7561 ttgacaatgg acgtattata ttagaacgtg aaaaagatac tctattggac gaacatagaa
     7621 aagtaaaagg ggatattcaa tttctaaatg atgaaaccaa aaaattattt ttaaatttag
     7681 aaattaccgg ctatggtttt atagggatta cctcaaaaat taatgaagtg caaaaaaata
     7741 tgccagatat tttaatagag cgtcctacaa ttgaagatat tatgcttgca tatattgaaa
     7801 ggagagagta attatgttga ttcatttagt taaaaaagat attctgattg taaaaaaata
     7861 tgtattgttg atattattta ttgcaatagg aatcccatta tttatattat ggcgtttacc
     7921 tccatttgca caaatattgg gatttgtact atcaattata ttttcagaat ttatgctgtg
     7981 ccagtatcta ttattaaagg agagtcaata ttcaaaagca tctgtattac tttgttcaac
     8041 tccttatcct agaagtggct tagttcaatc taagtatgtt ttatttgcaa tggtatttat
     8101 ctattgtaca ttagtttact ggattgaaaa tttacttatt ccacaaatag gaacttttga
     8161 cttaacatat attttgattg ttttattaat aagttctatt atttatggag tttatatgcc
     8221 tattcaatat aaattaggat atgaaacaac aaaattcttt ttcgtggtga taatcatggc
     8281 ttcatcattt gtattaccat taactatggc tcacagcaat tttgaaattc catttatata
     8341 tcagtatcca tccatgatag tgaatattgt attgttcctt ttaagtttaa ttatactgtt
     8401 agcttcaatg tttatttcta ttaaaatttt tagtaataag gagcttgtat aaggtaaggg
     8461 ggaattttat atgccacatt taccagattg ggctgtttta ctttgtatct tacttccaat
     8521 agtatataaa gcttgtcaga tatttaaaag taaaaaatag agtacatatt tatattgcca
     8581 tttttattga gtaaagttag atggtatgaa tttaaaaggt tacttgaaaa taaagttact
     8641 tggtgtggga gagtaacatc ataatattat agattatatg tttcttgtga atcaatttgt
     8701 attttttaat agggaatatc cagtaatttt attttccatt gattattttt caattatgag
     8761 tatgattgtg tgtattgtat accatctttt aaggtgtttt taaaaaatta tatcgtcaaa
     8821 atataaatct aaggaatcct ataaataaag cttttcaatt ttagaaaact aataaaaagt
     8881 aggaagttaa taaaaagaag ctgtctcaaa atagagataa gcttcttttt cattgctgaa
     8941 atgacgaaaa tataattgat ttaatataaa aaagagtgct ttatgaacta tttattttga
     9001 acagtagata cacttgcact caatttagtt actcctaagc tttatgaact aaaagtttat
     9061 tttgaagctt atttttatta aaagagattt tatttttcta aataatcatc tatatacagt
     9121 attgcgtttc ttttatttat tcataattta ttttcttatt aaagtttttc tatattcata
     9181 ttttccatat tactttactt ttaattttat acgaatatac gaattatcaa aaataaaata
     9241 gttattagaa aaaaataaaa gtaattgcaa agcttgcaat tacttacttc tatattaatt
     9301 actataccat aaaataacaa aaaaatcaag tcgagtaatc cttcaactta agtgatatac
     9361 taggtattaa taatttttaa ttaataaaat ataactagag gaaaatctac attttctcaa
     9421 tttctttaat taaattgacc tctaattaag taattataag gagaaaaatt atgaatttag
     9481 caaagggatt agatgtttta aaaatttcat caaatgtatt cggtgaagat aaagttatgt
     9541 atattcctgt aatctacaca gaagatgatg caacacttat tgacacaggt cttccgggtc
     9601 aaggtgattt aataattgat gctcttaata aaagtaacac gagctttgat aggttaaaaa
     9661 atat
//
LOCUS       DAEORZ010000148         9636 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEORZ010000148 DAEORZ010000000
VERSION     DAEORZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.151"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            33..1244
                     /locus_tag="KRH39_003175"
     CDS             33..1244
                     /locus_tag="KRH39_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG8513056.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            1437..1697
                     /locus_tag="KRH39_003176"
     CDS             1437..1697
                     /locus_tag="KRH39_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBG8513057.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            2051..3763
                     /gene="ptsP"
                     /locus_tag="KRH39_003177"
     CDS             2051..3763
                     /gene="ptsP"
                     /locus_tag="KRH39_003177"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBG8513058.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNIEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            4086..5636
                     /locus_tag="KRH39_003178"
     CDS             4086..5636
                     /locus_tag="KRH39_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8513059.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     ENLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            5662..8364
                     /locus_tag="KRH39_003179"
     CDS             5662..8364
                     /locus_tag="KRH39_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8513060.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNAYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVTAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVESFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVQVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDIILKEVSSKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTIKIKDKNIDFEQMQL
                     NLSVQQIVHKQCANDILSCIENCGLDKKYISVEVTETVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQIAKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(8870..9535)
                     /locus_tag="KRH39_003180"
     CDS             complement(8870..9535)
                     /locus_tag="KRH39_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513061.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
BASE COUNT         3788 a          924 c         1643 g         3281 t
ORIGIN      
        1 tcagaaacgt ttttgatata gtaggtgata atatgaaagg attagtttta gaaggtggag
       61 gaacaaaagg agcctaccaa ataggtgcat ataaagcttt aagagagctt ggtatagagt
      121 ttcaaggagt aactggcaca tcaattggtg ctttaaatgg agcatatata attcaaaatg
      181 atattgaagt aatggaagat atttggttaa attatgacta tactcatttt atgaatattg
      241 atagagaaac ctatgagagg tttaaaaatg tagatttcac tactaaaaat ctcaatgcag
      301 tgtttgagct tataaataaa gctagaaaaa atgaaggtat agatataagc ccattaagac
      361 aattaatgga gacaacatta aatgaagaca taataaggaa atcaaaaaaa gactttggac
      421 ttgttacggt ctggtgggat aaaaaaatta atccatatcc attgtatatc gaagatatac
      481 ctaaaggaag attgattgat tatttaattg ctagttccag tttgcctatt tttcaaacga
      541 acatgatgga tgataagttg tatcttgatg gaatgttttg tgataacatg ccaataggac
      601 tcttggaaga caaaggttat gaagatgttg ttgtaattag gttgattgat gattttattg
      661 ggaaaatgaa tctaaatcga catcaaaata taaatataaa aactataata ccatcagaat
      721 atctaggagg ttctcttaat aaagatagag ataatgtaga aaaaaacatc cgactaggtt
      781 acttagatac tatgaaagca tatgaaagat gcactggaat taagtattat tttgatgtag
      841 agtataaata taatgaagaa tattgttttg ataaatttag aagtatgagt aatgaaacaa
      901 tacaatatat ctgtaattta ttgaatataa aaagagatgc ttcacttaga accgttatgg
      961 aaagtgtaat accaaagctt ggtgaggtgc ttggattgcc aaaggatttt tcatataaag
     1021 atttgtttta ctgtatatat gaaaagaaac ttgaagaaaa taatataaac aggataaaat
     1081 tatatgattt taacaaggtt atagatgttg taaatacaaa tattgattct aatagaaatt
     1141 tagcttctaa ttttgaagtt agacctaaaa ttagtattaa aaagaacaaa aacacaaaat
     1201 tattgacaaa ttgtattata aaagacataa aaaatcaaag ttaaataaaa aacaaaaaaa
     1261 aatttgtaat tatactcgaa aaaagtgtat attagtagta taatattttt gtagcactag
     1321 tgggaacaaa aaatgaccat aggacaaaaa agtaccactt agtaccatta aagtaaaaaa
     1381 caggaaaaca attttaaaaa atataaaatg taaagtttat aggaggattt aaaaaaatgg
     1441 aaaaagtagt tagtatcaaa aatgcaagtg gattacatgc tagaccagca ggaatgtttg
     1501 ttaagaaggc agctgaattt aaatcaacag tagaggttgt agcaaaagac aaaactgtaa
     1561 atgcaaaatc aataatggga ataatgagtt taggtttagc tcaaggagaa gaattaaagt
     1621 tagttgtaaa tggagaagat gaagaagtag ctttagctgc aatggttgaa ttagttgaaa
     1681 gtggatttgg agaatagata taataatttt ttaaaaattt gatatttaga atctaatatt
     1741 acttaaagta aagtaacaga gaagtattaa acatatacag accaacattt gtagatattt
     1801 aatttctttg ttactttcat gtttatttaa tcagaagatt aagactattg ttaattgggt
     1861 ataaaaatat aaatatttta gaaaatgaaa taagatattt ttaatcataa attcagagta
     1921 aaaatacagt aacaaatagt ttataaaatt ttataaaaaa ttataagtaa ataaaactat
     1981 aaaaatgtaa attatttata taaatttata aagtttatgt gctagatgat attttttagg
     2041 aggtagggtt atggcttaca aaggaatagg tgcttctcct ggtgttgctt tagggaaagc
     2101 gttagtagtt gaacatagtg aattagtaat tgaaaaaaaa tctatagata atattgaagc
     2161 tgaaattgct aaattagaag atgcagttgc agtttctaaa gaagaattag taaaagttaa
     2221 ggaaaaggct tctgaagaat taggagcaga ggaagcagaa atatttgaag cacatttatt
     2281 agttttagaa gacccagagt taattggttc tgcaatagat aaaattaaaa ctgaaagtgt
     2341 aaatgcagag tatgctttaa atgaaataaa agagatgttt gttagcatgt ttgagtctat
     2401 ggacaatgag tacatgaaag aaagagcggc agatataaaa gatgttacaa atagaatact
     2461 tagacatata ttaggaatta aagttgttga tttatctgct ttaagcgaag aagttgtatt
     2521 aatagcacat gatttaactc catcagacac agcaactatg aataaaaaaa tggttttagg
     2581 atttttaact gatataggtg gaagaacttc tcatactgct atcatgtcta gaactttaga
     2641 aatagctgca atcgttggat taaatgatat aacttcaaaa gttaaggatg gagattttgt
     2701 ggttttcaat ggagacacag gtgaagtaat cgtgaatcct gatgaagaga ctataaataa
     2761 atatactgaa ttaaaagcta agtatgaaga tgagagaaaa gcattacagc tattaaaagg
     2821 aaaaccatct gtaactttag atggaaaaca tgtggaatta gctggtaaca taggaacacc
     2881 taacgatata gaaggtctaa taaagaatga tgctgaaggt gttggtcttt atagaacaga
     2941 atttttatac atggatagag attctttccc aacagaagaa atacaatatg aagcatacaa
     3001 agctgtatta gaaggaatgg atggaaagcc tatagttata agaacattgg atataggtgg
     3061 agataaagaa ttaagttatt taagtatgga acctgaaatg aatccattct taggttacag
     3121 agcaataaga ctttgcctag atagaaaaga tatatttaag actcaattga gagctttata
     3181 tagagctagt gttcatggaa gattaagaat catgttccca atgatttcat ctttagaaga
     3241 gttgttacaa gctaaagaag ttgtaaaaga agttttagca gaacttgatt ctgaaggtgt
     3301 ggcttatgct aaagatgtag aaattggtat gatgatagaa gttccatctg ctgctgtgat
     3361 atctgatgta ttggctaaac acgtagattt cttctctata ggaactaatg atttaattca
     3421 atacacttgt gcagttgata gaatgaatca aaagataagt tatctttaca atcaatttaa
     3481 tccagctgtt ttaagactta taaagactgt tatagacaat gcacataaag aaggaaaatg
     3541 ggcaggtatg tgtggagaat ctgctggaga ccaaaaaatg ataccaatac ttttaggtat
     3601 gggattagac gagttctcta tgtctccaat atcaatactt cctgctagaa agttaataac
     3661 ttcagttaaa gagtcagata tgaaaaaatt agctgatgat gtacttaata tgggtactgc
     3721 tgaagaaata aaaagctata tagaaaagac ttttaatata taattaatgt tatatagtta
     3781 ctaaaattca tttttctaaa taacaaaatt atattttacg aataaaaaaa ctatagtata
     3841 tcatatatac tatagttttt ttgttatata aattacgtat caaaatttgt aaaatgaata
     3901 gatataatta ctaaatatgt gatagtattg gctataatag gatataaaaa ataatattgt
     3961 aataaaaagt ttatatttag taatgaaaat ttaacgtttt acttgaaatt atgtgttttg
     4021 taatgtatta ttaaggttct aataggaaca ttttcatcaa tttttctata gtgggggtag
     4081 gaattatgag gtacaaaaaa agaaatatag ataagtcaag tgaactttca tttcaatctc
     4141 tagcaaaata ttttataact ttctatatag taattatatt aattattctt tctatgtttt
     4201 gtataaatac aatatgggtt atgaaaaaag ataatcaaga tttattatct acaacatcaa
     4261 atcgtattga agatagggta ggagaaacaa ttaagctatt gaactctttg agtaaagata
     4321 attacataat agataatagt gtgaatggca tagataaaag taaagaactt tcaatgtata
     4381 gtgatgaatt tgggtatatg atgatatgtt ttgttgataa gaatataatt gtatgggatg
     4441 aagaaggtgg taatattagt ctagctggtc gtgattatat gcagaaactt tttgcaacaa
     4501 aaaaatatca agttacagat agttttgcag caggggctga tggaaaaact ttaaactaca
     4561 ctattgcatc acctataata aagaatggaa atgtagatgg ttgtgtattt gcagcaattt
     4621 actttgatga gataaaggat atactcaaac aaggaaaagg tcaacagcaa cagaatttca
     4681 tattatttgg aagtgaaaat caaataatga atttaacaag tgatgaaact gcatatggag
     4741 agaagtatat gaagttatcg cagaaaacaa aatatcttgg aaccaatgca aaaaaagtag
     4801 aaaaggatat gtttaataat aaatcaggtg attattgggc ttttgaaaat ggaaaattat
     4861 actttatgtc ctataaacca attaagaaca caaattggag tttattttgt aaagttaatt
     4921 tcttagatat ttttgcatta aatttaattc ctatgggagc tattctatct gttataactt
     4981 tagtatttat tattttgatt tgtatggaga aaagcttcat gaaaaaacga atggaagtta
     5041 ttgatatgct tttaggttct gttcaagaat tagaaaaacg tatatatcaa acagaaagac
     5101 ctagtgaagt agactttaaa gaaattattc agctcacaag taaaggtctg actgatggat
     5161 taactggtgt tgtaacacgt actgtatttt ttgaccaaat ttataatcgt gttgaaatgg
     5221 aggaaaatga taaattatct gtaatttcat ttgttgattt agatgatttg aaatgtatta
     5281 acgacttata tggacatgaa attggggata tggttttgaa aaatgttgga tatgtacttc
     5341 gtgaatatga gataaaatat aatggtttaa ttgggcgcta tggtggagat gaatttgtta
     5401 tgttgttatc tggttttaca tcgagagaag aggtggagaa tttattggat gagattgcta
     5461 ttcgcttaca tactgaagta ctaacagatg atggtatcat tcaaactcat tgtagtgttg
     5521 gagctgtagt atgtagtgta aaagacaata aagatataga taatttatta aaacaggctg
     5581 atgaagcttt atactatgtt aaacgtaatg gaaaaggtaa ttatcattta gtttaatgta
     5641 tgtggaagga gtttatatca tatgaaaaat gtatctaaaa aattttttgt atgtactatt
     5701 attgtattaa taacattttt atgttttagt aaatttgcta tttatgtttt tgacaaacaa
     5761 gaaaagaaag aaacaaaagt agtagaaaac atgttaaatg cctacaagaa taacattatt
     5821 ttaaatctga ctgaaaaatt taattatagc aaagacttga catcagtttt agtttcaaac
     5881 aattattcta tagataaatt aaaacctatt gcagatgaaa ttcttaaaaa caattcttgc
     5941 atatatattt cagttgtaga aaaatataat gtaactgcat ttttttctaa ttctgtcaaa
     6001 aaagatatta tcaatactcc aatacaggaa ctgaattatt cttattcatt agcaaattct
     6061 atcaaaggat tagtcgtaga aggtccttct aaagaccagt tttcaaatgg agatgtgttt
     6121 ttaattacta atcctattta taaaaatgga gagtactggg gagaaattac agtagcttta
     6181 gatgccaaaa aagttgtaga atcttttaac ttaaataaat taaaagaatc tggatttgag
     6241 tatgaacttt ggcgtgtaca cctagataat ggttcaaaat ctacaataga aatttctact
     6301 aaagatttta atttttccaa atcacatgaa attaagttta aacttccgac tgtttggact
     6361 cttagcattg tgtacaaaaa tggatggaat aataagaata tagattttat tgtatttgtt
     6421 atattgttaa ttatttccat attaataggt gtgatgacat ttatttacat tgaaaagaag
     6481 tcgattagta aggaattaaa tcaacagagt cttaaaaatt tagaaactgg tattttgaat
     6541 agagaaggtt ttttaattaa gttacaagag catctgaata gaaaaaataa tgaaatagtg
     6601 ttgctgtata tatgtattac taattatagt aaatttgctc cagttatgag ctttgaaaaa
     6661 agacaagatt atcttacaca tatagtaaaa aatctagata attacataaa aagttctcat
     6721 tttattgctc gtataagtga ggaaggtttt gttctagctt tagaagaaaa ttgtgataaa
     6781 aatgaattgc ttaacattga aaaagggatt tccttagagc ttttacgaaa aattaaagta
     6841 gaaaataaac aagtattttt aacgattggt tatggctcat ctaggtgtgt tgatgataga
     6901 aaagatgcag aatctctgtt actttcagct attgaagatt atgagagaag atttccagca
     6961 atgtctccta ttcatgattt tactaaaaag tgtgaagata tgcttgatga aaagaaacag
     7021 attgtatttg gtgaatatcc agatttagaa ataaacaagt tgtctaaagt aatatacaaa
     7081 tattataaaa aagtacagcg tattatgtat aaagattata atattgatat tggaaataga
     7141 cgagggtata tgcgtgatgt tcaagtatta attgattata attctgagag aatgttatac
     7201 ttatttttaa tagacatatg caagtttgga aaatttaatg aactgttttc tattgaaata
     7261 ggagatatta ttttaaaaga agttagtagc aaattaaaaa caattttttc taattatcta
     7321 tatcgtatta atggagatgt atttttaggc atttcaatag ataaagaatc ttacttagat
     7381 actttacata aggttaatga agtatttaat agttctttaa gtgtagaaga tgtacttttt
     7441 gatgtaaaat gtaatattgg tatttgtcaa tatccatatc atggtgaaaa tccatatgac
     7501 cttctggaaa aggtacaaat tgcattggaa tatgcaaaaa aggatgtcaa aagtaatgtt
     7561 aaagtttatg ataaggaatt gacagaagag atttaccatg aaaataatat tattaaggat
     7621 ttaaaaaaag gtctggaaga gcaaacacta gaagtttggt atcagccaat ttttaatgtt
     7681 agtaaaaact gttatataaa agctgaggca ctaattagat tgagaaataa agagggcggt
     7741 tattattctg cttatgaagt tattacttat gcagaaaagc atggacttat taaggatgta
     7801 ggtctatatg tactaaaaaa ggcttgtgat gtaactataa aaataaaaga taaaaatata
     7861 gattttgaac aaatgcaatt aaatttatct gttcagcaga ttgtacataa gcagtgtgca
     7921 aatgacatat tatcatgtat tgagaattgt ggtttagata aaaagtatat ttctgttgaa
     7981 gttacagaaa cagtacttat ggaaactttt gaaattgtaa atgttactct tgataaacta
     8041 aaaaaagaag gcattagcat agctttagat gagtttggtt ctggatttag tagtataaat
     8101 tatctggcaa atttacctat tgaaaattta aaaatagata aggaattagt agaacaaatt
     8161 acctattcta aaaaacaatt tgaattatta aaaactatag ttcaaattgc taagattaat
     8221 ggcatggata ttgtagcaga aggtgttgaa acaaaagaaa ctctggattt agttgtaact
     8281 tcaggtgtga atttgattca aggtcattat ttttcagaac ctttaagtga agataaattt
     8341 attgaactta tgattgaaga ataaaggtta ataaaataaa gatttaaggt attgaaaaat
     8401 aattaaaagt atttaataat agaattaggg aagaataagt ttataattaa aaataattga
     8461 ttagtagtta ataaaataaa tgtgatatcc aaataaatcc taaatctaat ttgagtttta
     8521 atatagatag tctaaagttt gattattaaa gatttaattg taagttgtta cagttgatga
     8581 tattgattag agattaaagt ttagagtaga aagttacctt aaatttaaaa atgcaagata
     8641 acccaatcta aaaattataa tcactcaata ggtttaagga tttatttgtt tattataaat
     8701 aggagggtgt ctcaaaatga actttttagt ttatgaggca ctctctttta tatagaatta
     8761 aatatatttt attatttcaa caatgaaaaa gaagcttatc tctattttga gacagcctct
     8821 ttgtatacta ttattaattt aatcaataat ataatttaaa attatattat tagttaagat
     8881 ttcctgcttt attagtaaat aattctttaa tagcattgtt tctggaaact aaatcgttat
     8941 cagagttgtt tgtttttttc ataatcactg aataacgttt tttatttata aaaaatatta
     9001 aaatcttatc atcatcaaaa ttgtattctt tatatctgtc aatagactca taacctagat
     9061 ttagaatgtt tttgtctttt atactatcta catctaaaat atatgatggg tcagccttta
     9121 gtaatatatt aattaaacat ttttcttcac cagcacagaa taactccatc ttacttgata
     9181 tagatttaga ttgctctgaa gttatttttg caggaataag gttgtcatta tcatctctta
     9241 gaagataacc atgtgtgtct tgttgatata ctaaattaac ctttttctta ggaacactaa
     9301 tcattttatc taaatgtaca taatgatgat tttcaacaaa accttcattt aatatttcaa
     9361 caaagttatt aattttacct aggcaaaaag attctataga ttctttacag aatttatttg
     9421 taaatggtat tatgtttacg ttttcctcgt ctgtattcca tgctatagca taatccaacg
     9481 aatatacttc ttcctctata tctgcaaaat tatattgtac tatgtttcct ttcaaaatta
     9541 ctcgacctcc atcttataat atatttaata tactttataa aacttatatt atatttaaat
     9601 aatatcataa attgaataaa tatgatatag taaata
//
LOCUS       DAEORZ010000149         9598 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEORZ010000149 DAEORZ010000000
VERSION     DAEORZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.152"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            49..1077
                     /locus_tag="KRH39_003181"
     CDS             49..1077
                     /locus_tag="KRH39_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513062.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLIHQVSSIDELKAKLDEDTNAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKEMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNVGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSRLKNAFFMNFLKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            1080..2093
                     /locus_tag="KRH39_003182"
     CDS             1080..2093
                     /locus_tag="KRH39_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513063.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTIWPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEILPQSRSKYIIQKVFLGLLCGCLSSTITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGAPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVAWFMLYIILDKKRKLS
                     FSR"
     gene            complement(2625..3875)
                     /gene="cme"
                     /locus_tag="KRH39_003183"
     CDS             complement(2625..3875)
                     /gene="cme"
                     /locus_tag="KRH39_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBG8513064.1"
                     /translation="MENKNTNSTWKKKVILFLTSQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDATIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGIQTPAVNAMIPQLVPEEHLMRFNGINGSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIRFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVMSINNLIIVAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(3961..4707)
                     /locus_tag="KRH39_003184"
     CDS             complement(3961..4707)
                     /locus_tag="KRH39_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8513065.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNKYLQQLEIEKSN
                     AEEALTIVTQILNGNTPETDKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(4822..5844)
                     /locus_tag="KRH39_003185"
     CDS             complement(4822..5844)
                     /locus_tag="KRH39_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBG8513066.1"
                     /translation="MNKNNCLDLIFEKVNPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEVCKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSTREFANEYLFKPIGMKIIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSTGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(5844..8264)
                     /locus_tag="KRH39_003186"
     CDS             complement(5844..8264)
                     /locus_tag="KRH39_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG8513067.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRARITPGYLRVFSKKFNDYLDIQFLQLPEEVRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKILIDQGNLNTFGTNLVNDILSKTRHLIESRLGHLSLYQEM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYISGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQTNSSESEIGDEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACSKCSGSGYEKVWLG
                     NNLSIDHTCSECQGKRFNDESLSIKYKNKNIHDILNMSVSEAVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTAK
                     LIQLLDELVSNGNSVIVVEHDIDVLNVCDWIIELGPEGGDKGGYIIAEGSPKVLKENP
                     KSITGRYL"
     gene            complement(8350..9270)
                     /locus_tag="KRH39_003187"
     CDS             complement(8350..9270)
                     /locus_tag="KRH39_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8513068.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTITDIAHYFGFTDSAVFSRAFKN
                     HYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSDGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         3208 a         1383 c         1454 g         3553 t
ORIGIN      
        1 tacactagaa gaagttttta ttaaatatac aggaaggatg ttagtgtaat gtattctaaa
       61 ttttttacct tagtaaaaaa agatttttgg atgatgattt atggtaaatt ttttgtacta
      121 acacttggct ctcttatact ttattcgtgt tatattaatt ttggttatgt aaagtttatg
      181 gatgaagata gttatcacaa taatgtatat ctatataatc cagagaatat aagcatgagt
      241 gcatctccat taattcatca agtttcttct atagatgaat taaaagcaaa acttgatgaa
      301 gatacaaatg cagtttgtat tgatatgagt tccagtaagc caaaagtaat tttacatgaa
      361 agtattaaag gagttgacaa ttatagagct gactatgcat tgtcattatt atcatcagaa
      421 aaagaccata atagcaaaat atcggacaac aatgcaataa ttcaaattaa tgataaggat
      481 acatctgaga taaaacaaag aaaagaaatg acttgtgaga ttttattctt tgaaattgta
      541 gctattggat ttttagggat tgcctcttta ttatttaaag aaaaacagat gggggtaatg
      601 cgtattactg gtattttacc tgtaaaaaga agccttttta tattatcaaa gatatttgct
      661 tttttgatat gtgacctatg ttttacagtg cttttagtag tatttaatgt tggatttttt
      721 gaaggaataa aaatacttcc acaagtatta gttcaagcta gtatcttatc aattattatg
      781 tctttgattg gaataggatg ttcaatgtta ttgagagatt ttaagcaatt ttctcttata
      841 taccttgtgg ttgctgtatt cataactaca cctatatttc tatctgcaaa tacatctttt
      901 gaaatagatt ggattaaata tcatcctttc tatcatgttt atagtcggct taaaaatgcg
      961 tttttcatga attttttaaa agaattagtt tattttgtag tgtgttcttt gacaataata
     1021 gttctatttt tacttgtaaa atatattttt aataaagagt tggttaggga gggataatct
     1081 tgataggatt acgttaccag ttaaagagta taaaaaaaga taaaatgtgt ataatttcct
     1141 tctttttacc agttgttatg gcgattttaa taaattttat gggaacaatt gatatatctt
     1201 cattaggaga gatacgtttt ggtatagtga aggatacact tcctgtacaa acagagcagt
     1261 ggttgaagaa gtatggtatg gtaagtgtat atgacacaaa aatgcattta atacgtgaaa
     1321 ttaaggatat taaaacggac acaattggag ttgttagaga tggtaaaggg ataaaaactg
     1381 tgctttatgg aaatgagatt aatgctataa aaaatgttgg aaagacattg ccagagatgt
     1441 ttagactaaa gggagattta aaagatgttg aggttacaat atggcctaaa gatagtattt
     1501 taaaagagtt tcaaaatgtt tttattgcta tgattttaat tactgctatg tttatgggat
     1561 gcacctttaa tgcaatgaat attatatcgg aaaaagaaga tggaatatca tttataaatg
     1621 aaattttacc acagagtaga agtaagtata ttatacaaaa agtatttttg ggtcttttat
     1681 gtgggtgtct atcttcaacg ataactgtat ttatatgttt ggatatttca atattagata
     1741 tgataatgat gttgctactc gttgtatttt cagcatttat ttcatcactt ataggtctat
     1801 ttattggaaa gttgtcagaa ggtcttatgg taggaattgt ttatattaaa atagtaatga
     1861 ttttatttat aggtgctcct ttgttaatat atcttctagg ggttaataca agtggtatag
     1921 ggaatttatg ctatataatg ccatcagttg ctacatttaa tggtattatg gagattatta
     1981 atggtaatgt cattattata aaagatattg taattttggc tgtacattgt gtagcatggt
     2041 ttatgttgta tattattttg gataaaaaaa gaaagttatc ttttagtaga taactcagta
     2101 taattataat taggacaatt taataggtga tttaatatac tttgtatagt gtaatttatg
     2161 tagtgcaatt tgtatactgt aatttatgta gtgtaatagt tagttttatt ttataattat
     2221 tcaatataat ttaataagaa gttcagtata gttagagcta cttagtggaa aatttaatag
     2281 gtacttaaac tatataaata ctttattttc ctccatttgt actataaatt taattgatat
     2341 ataaatggag gtattattat atgatttgat aaatttcatc attataactt acaagtaaag
     2401 gtattattat atgagatatt attatataaa gttaataaag atagtatcaa aatattattt
     2461 attataaaga gaggggctca aaataaagat aaacctattt ttcaatgttg aaatgataaa
     2521 aacatatttg attttatata aaaaattttc ttcataaatt aaaaagttca ttttgagacc
     2581 cctcattttt aactagataa atcgagttaa actatttaaa tatattactc agattcattt
     2641 gacttagata ttccttgctt gtaaaattgt tttgagtagg ggatagccaa agcaaaaaat
     2701 aaaagtataa tccctgctac gattattaaa ttattaatag acattacatc tgccattgga
     2761 ccgaaaatca tcataccaag aggaataaaa ccagtatata taataatagg caagctaaga
     2821 actcgaccca gcatttgtgg tgctacatgc tcttgcaaca tagtcatagc tgatgcttga
     2881 attattggaa ttgaaaaatt cgtaaaaaac ataaatataa aaaattgcca agcttgtgtt
     2941 acaagaccta gaataattga aaaaatagca taggataata ttgctagaaa aaaggtctta
     3001 tttctatttt taaaaccacc aaaaactcct ataatcaatc cgcctaaaac catacctaaa
     3061 aatctaatca tttcaataat agacaggtat atatagccct taccaaatgt tcgttcaata
     3121 agaagtccta taagaaatcc tgatggaaca caaagaagca tgaatgctcc ataagtgaaa
     3181 agtacttttc taatgaattt atctgaaaaa cagtacttta tccctgatac catatctgca
     3241 aaaaatgaga ctttttcagt ttgttttggt atatcatgtt taggaatttt gatgaatatc
     3301 aataatacaa tagcaataat tgcagtaaat acatcaatta aaagaacttg atttatttta
     3361 ttaccaaatc caagtattgc agcagccaca ataggaacta taaattgtac tatagactgt
     3421 aggctaccat ttataccatt aaaacgcatt aaatgttctt ctggtacaag ttgaggaatc
     3481 attgcgttta cagcaggtgt ttgtatacca cttcctagtg aacgtataac agatattact
     3541 gatatggcta ttaattcact tgttgtgcca tttgatgaca tcataaatag aattagaact
     3601 aatgtagata tagcaattgt tgcatctgat acaattatga gtacctttcg attgtagtag
     3661 tctgcccata caccagcaaa aaaagaaatt atcatttgag gtataaagga acatacagtt
     3721 aaaactgtta cccatacacc tgatgatgtt tgaagtgtga cataccatat cattgccatc
     3781 tgtacaagtg atgagccaaa taaggaaata tattgacttg ttaaaaaaag aatgactttt
     3841 ttcttccatg tagaattagt atttttgttt tccatcctat tcctcctttg atttgtgtgt
     3901 ttaatttttc tcgctcttaa ggataacaca caaatcaaag tctggagtta gagtggaggg
     3961 ctaggaaaat tgttttttca taatttgaag atgagatttc atggtttttg cattatgttc
     4021 agcttctttc aatgaagtta tcagtttgtc acacactgag ataatttcgt catcatcttt
     4081 tggagtgtca aggacagttt taatattagt attaggatta gaagataaag catttaacat
     4141 tcgtaaaata gctgaaagag aatagtttgc acaacgaagt gcgcttataa ttttaagtcg
     4201 ctttatatct tcatcagtat atacacgata accattttgt tttcttttta cttttaacaa
     4261 accattcatt tcccaatttc ttaaagtatc tattgtaaca tttaagtgtt ctgcagtttc
     4321 ttttctagtt aaaaagagtt tatctgtttc tggagtattt ccatttaaaa tctgtgtaac
     4381 aatagtaagt gcttcctcgg cattagattt ttctatttct aattgttgca agtatttatt
     4441 tacaagacta atagccttat caaagtccct atttgctgat gttcttacaa tctcaagagc
     4501 atcttttctt aatcctcttt gtaccacttc aattttcaag gctgtacgag caaatctaaa
     4561 ttgttctata tgaaaatcat taaagatacg ataaccattg gtattacgct taggtttagg
     4621 tattaagttt agttcttcat ataaacgtac tgtattagga tgtacaccaa ttaaatctgc
     4681 tacctctgat gtcgtgtaag tgtccattag tattcaactc cttttagata tatagtacta
     4741 atcataataa caccaaacaa aagtctggtg ttatagtgga gggtttggtt atctaagagt
     4801 aattagtttg tctaagagtg attaatctat tatagaagta agtatatatt tttcaataaa
     4861 ctcccatcta tcacgaggtt taaacactat tttagatggt atggctatta ctaagtcata
     4921 ttgtggaata caacaaatag catttcctcc agttcccatt gccgaatacc acaaaactcc
     4981 atctttttta catagccacc acagataacc atattcatta ctattcattt taactgattc
     5041 ttcaatccac gatttagata taatacgttt attattccaa ataccatcat ttaaatacag
     5101 aaaaccaaat cgcaacatat ctctagcact caatgtaagt ccccagccac ctgtagaata
     5161 gccctgtcta tcttttgccc aacccttaag attttttcca aagaaatctt caaaaccaaa
     5221 atctttcata tcataatctg gtataatttt cataccaata ggtttaaata agtactcatt
     5281 agcaaattca cgagtagatt ttccagtagt ttttgttata atagctgaaa gtaggtgtgt
     5341 tccagctgaa gaatatttga aagtaccaat ttcacctttt tgaccaagct gattcaaggt
     5401 atattttatc caatcttttt gtttgcaaag tctctctaaa gactcagtca tatttttaaa
     5461 aggatatggt gcagtcattg taagtaggtg acgtatagtt actgcttttt tacatacttc
     5521 agaaggacta caagtatatt cagggaaaaa atcaacaacc ttttggtcaa catcttttat
     5581 atatccttta tctattgcaa tgcctattaa ggctgatata atactttttg taactgatgc
     5641 aacatgaaaa gtatcatcag cattatatcc attatagtac ttttcaaaga caacttttcc
     5701 ttttctagca ataattattc catttatatt actatattgt gagattatta agggttcaag
     5761 ttgtgaaagc ttttctgaac tcataccaac acttgaaggg tttacttttt caaatattaa
     5821 gtctaagcaa ttgtttttat tcactaaaga tacctccctg ttatagattt tggattttct
     5881 tttagaactt ttggagaacc ttctgcaatt atataacctc ctttgtcacc accttctgga
     5941 ccaagctcaa taatccagtc acaaacattt aatacatcta tatcatgttc aacaacaatt
     6001 acagaatttc catttgatac tagttcatct aataattgaa tcagcttagc agtatcgtat
     6061 agacttagac cagtagttgg ctcatccagt acataaagga tattaccatt tcgtttttta
     6121 ccaatttctt ttgcaagctt gattctttgg gattcaccac cacttaaagt cggtgtaggt
     6181 tgacctaatt ttatataccc catacctatt tgttctaata catttaagtg tgaaacaata
     6241 tttggaatat ctttgaagaa atgtacagct tcagagacac tcatatttaa aatatcatgt
     6301 atatttttat ttttatattt aatagataaa gattcatcat taaatctttt tccctgacat
     6361 tcactgcatg tatggtctat acttagatta tttcctaacc agaccttttc atatccacta
     6421 ccactacatt tagaacaagc acctttggag ttaaatgaga aatgaccagc tgtaaatcct
     6481 cgttctaacg attctggctg acctgcaaat agtgtacgta ttttatccca tataccaata
     6541 taagaagcag gagttgagtt catatttttg cctataggag attgagatat ctctgaatat
     6601 ccagaaatat gtttagtacc aatcagtcta tctgcaatag tttcaacaac tgcataatcg
     6661 tcttcatcac ctatttcgct ctctgaagaa tttgtttgat tattgatagt atggttatta
     6721 aattggcttc taagaagagg aagtagggta tctgatataa gtgaactctt gccactacct
     6781 gattttccag atattccaac catagcaccc aaaggtaatg atacagttac atttttaaga
     6841 ttatttgtat ttgcattttg aatagttata caaggtattt tatcagtatg tgtaatattt
     6901 ttattgcttg ttctattagg cattggatat tttccagata tatattgtcc agtaagtgat
     6961 ttgtcggatt gaagtagacc ttctaaatct ccttgatata ctaattgacc accttcaaca
     7021 ccagcctttg gaccaatatc aataatatgc tctgccatct caattgtgtt tttatcatgt
     7081 tcaactacta ttaccgtatt gcctaaatct ttaagtttct ttattgattt taagatgtct
     7141 attttttctg attcatgtaa tccagctgtt ggttcatcaa gcacataaat taatgagtcc
     7201 atctctgaat cgagatgtga atttaaaaat agtctttgca tttccccacc actcaaagtt
     7261 gacatttctt ggtataagga tagatgacct aatcgtgatt ctattaaatg tcgagttttg
     7321 gataaaatat catttacaag atttgtacca aatgtgttta aattaccttg gtctattaag
     7381 attttaagga aatcatcaat ttctaaaatc gtcatttttc caagttctcc aatatgtttt
     7441 ccgtttaaaa gtactctacg tgcttcttca ccaattttaa aaccattgca atctgaacag
     7501 gcagtcatag tataaactcc atttaaatct tcgccccttt tgtaacgatt gtgaaatacc
     7561 tttgataaag aggaactttt tttaccattt tcataatgac catagagtac ttcatttctt
     7621 acttcttcag gtaattgtaa gaattgtatg tctaaataat cgttgaattt tttagagaat
     7681 actctcaaat atcctggtgt aatccttgct ctatctaaga cttgttttag ggtatcatgc
     7741 ttatcaagga ctaatttttc aatattaatc tcaaaatatg caccatgtcc agagcatttc
     7801 atacacattc catctgaaga gttgtatgag aaataacttg gtcttaatcg ttcttcttca
     7861 tacccacaat tattgcaaat taaattgtcg cttacaggag tatcacacat agtacacatt
     7921 atttgacctt ctactgaata caatagtgta agcatgttga gaatattggt tcttgtgcct
     7981 actgttgaac gaggattact ttggcgaata atattttgtt gaacagctat agtgggagct
     8041 aaaccttgaa tattatcaaa cttatcctca ctatcaatac ctgcaagtat acctaaagat
     8101 tgcagatatt gtttacgccc ttcctcaaat acaatgtcaa acataaggct agatttacct
     8161 gagccactga cacctgttgc aacaacaagc ttatctttgg gaatagaaac atctatattt
     8221 ttaagattgt gtatctttgc tccattaatt tttatttctt tcattggttc acctcatttt
     8281 atcaatttta tcaaaccgta aaatagggat gtattaatcc ttaatacaag aatagattag
     8341 gtgaggttat taaaaaggtt ctataggtaa atatatatcg accaagtgtc tattttgagg
     8401 gtgattgtgt gggttgttaa gatatacttc aaaagggaag gaggcacgta atttgtaatt
     8461 accatccgat agccattcat tatacataaa atcccacgca tcactatatt cagattttga
     8521 aatatcaaaa tgtcccacaa gatattttcc agaaggtatt atcatattgc caagattgtc
     8581 attctctttg acaacaatgt tatctggaat ggtcatgcaa atactagtcc ttaattgatg
     8641 ctcctgtgta aattgtggat tatcatgata aatggctaat atttgtgtgt tttcaagtcc
     8701 aagtagattt tgctcacttg catatgtgaa taaccgttct aaaagttttg ggaaagtact
     8761 ggttagatta ccatatgaac cagtatatct agtgtaaata gtattcatat tatcaagttc
     8821 tagaatttca atttcacctt taacctctct agtattctta catttaggtt ttgatatgct
     8881 tctattgtac tgagaaattt tatatggttc tttgcaattc ttgctataat tatttctata
     8941 ttttatagga cttattttat agtgattttt aaatgctctc gagaaaactg ctgaatcagt
     9001 aaagccaaag tagtgtgcaa tatcagtaat ggtcatgtca gttcgatgaa taagaaaagc
     9061 agttgcattc tctagtttta gcctatttac atattgtgcc aaagtttctt tttctattgc
     9121 tttgaagatt ctatgaaaat gaaattttga aaaacctgct atattagaaa gttcatcaag
     9181 agaaagaggt tcatgtaaat gttcttctat atagtcttgc actttgcaaa tacgatttag
     9241 atattctttt ttacttttag catttgtcat atatagccct ccttattgtg taaaatgtgt
     9301 atcaattaat ttatagcaat atagtaataa aattttatgt ttttgtaatt tatgataaat
     9361 aagatagcgt ttaatttttt ataattgata aaagatataa gatgccttat atttgtcttt
     9421 gcatataagt atagtcttcg taataattag tataccatag tgatttattt ttaattgtgt
     9481 atttatataa atacacaact tatgatatgg acgaattatt atgcaaataa gctgtaatta
     9541 aataaattgc aatataaacc agttgaaaca taagttaggg aaatattatc aaagttat
//
LOCUS       DAEORZ010000150         9561 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEORZ010000150 DAEORZ010000000
VERSION     DAEORZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.153"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..862
                     /locus_tag="KRH39_003188"
     CDS             <1..862
                     /locus_tag="KRH39_003188"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBG8513069.1"
                     /translation="LILKSGEETKSFNTLPKIYDSLLDFKLTRKDLIITLGGGVIGDL
                     GGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFYQPKAVFIDPN
                     VLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYIIHKCCFIKKT
                     VVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKITLESEKIGITK
                     KGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILLKDIGESIIYN
                     TTSKFFQEVR"
     gene            862..2175
                     /gene="aroA"
                     /locus_tag="KRH39_003189"
     CDS             862..2175
                     /gene="aroA"
                     /locus_tag="KRH39_003189"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBG8513070.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGKTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            2153..3232
                     /gene="aroC"
                     /locus_tag="KRH39_003190"
     CDS             2153..3232
                     /gene="aroC"
                     /locus_tag="KRH39_003190"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBG8513071.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNMIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            3235..4431
                     /gene="pheA"
                     /locus_tag="KRH39_003191"
     CDS             3235..4431
                     /gene="pheA"
                     /locus_tag="KRH39_003191"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBG8513072.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKNFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            4478..5287
                     /gene="aroE"
                     /locus_tag="KRH39_003192"
     CDS             4478..5287
                     /gene="aroE"
                     /locus_tag="KRH39_003192"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG8513073.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDIKIDNSILDDIYNELKLEFL"
     gene            5299..5832
                     /locus_tag="KRH39_003193"
     CDS             5299..5832
                     /locus_tag="KRH39_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBG8513074.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAIY
                     NITNAVSENFKFEFKEK"
     gene            5853..6692
                     /locus_tag="KRH39_003194"
     CDS             5853..6692
                     /locus_tag="KRH39_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBG8513075.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            6960..8441
                     /locus_tag="KRH39_003195"
     CDS             6960..8441
                     /locus_tag="KRH39_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG8513076.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTITGWIILSFLFLGIIIYILYSKAKSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFLKKDFIANIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            8474..>9561
                     /locus_tag="KRH39_003196"
     CDS             8474..>9561
                     /locus_tag="KRH39_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBG8513077.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSNYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIILIIYIIYKNNKSEKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTRE"
BASE COUNT         3913 a          942 c         1640 g         3066 t
ORIGIN      
        1 tttaatatta aaatctggtg aagaaacaaa atcatttaat actcttccta aaatatacga
       61 tagtttactt gactttaagt taactagaaa agatttgatt attactttag gaggaggggt
      121 aataggtgat ttaggtggat ttgcagcagc aactttttta agaggtataa gttttattca
      181 aattccaaca tctttattag cacaagttga tagttctgta ggtggaaaag ttggagttga
      241 ccttgagaga ggcaaaaatt tagtgggaag cttttaccaa ccaaaggctg tgtttatcga
      301 tccaaatgta ttattatcac ttccagaaaa attctataga gatggaatgg cagaagttat
      361 aaaatatgga tgtataaaag acaaaacttt tttcaataaa cttttaagtt taaaaaatag
      421 agaagaagta atgcaacata tagaatacat tatacataaa tgttgcttta ttaaaaaaac
      481 tgtagttgaa gctgatgaaa aagactttgg agatagaatg cttctaaact ttggccatac
      541 attaggtcat gctattgaga aatactacaa ttttacgggt tatacccatg gagaagctgt
      601 tgccatagga atgtataaga taactttaga aagtgaaaag ataggaatta caaaaaaagg
      661 gacatcagaa cttataaaat caattttaat gaattataat ttgccttatg agattgaatt
      721 aagagataat aaatctatag tagatgctat atctcttgat aaaaaaaatc taggtgatac
      781 attaaaaata atacttttaa aagatattgg agagagtatt atatataata ctacttctaa
      841 attttttcag gaggtaagat aatgggaagt ctaaaaatat atccaagtaa attatctgga
      901 gatgtaaaaa taccaccatc aaaaagtatg gcacataggg cagttatttg ttcatctcta
      961 agcaatggaa aaagtagaat ttctaatata gatttttctg atgatataat agctactata
     1021 agagcaatga catcattagg agctattata gaaaagaaag aagatatttt agaaatttcg
     1081 gggatttttt ctaaagaagg tattttaaat agagagaatc agttgaatca acctaaattg
     1141 acaatagatt gtaatgaatc aggctccaca ttgagatttt tagtacctat atctttaaca
     1201 tttgatggtg taaaaaggtt tataggtaga ggtaatcttg gaaaaagacc acttgatact
     1261 tattatgaga tatttgacag acagaatata aaatatagtt acaaagaaaa tcagttagat
     1321 ttgattatat caggtaagct taaaccagat gaatttagag taaaaggaaa tataagttct
     1381 cagtttatca caggtttact gtttatattg cctacgctag agagtgattc aaagataata
     1441 attactactg agctagaatc taaaggatat ttagatttaa ctctttcaac gataaaagat
     1501 tttggggtag aaattataaa caataactat aaagaattta ttataaaagg aaatcaaacg
     1561 tataaagcga gagattataa agtagaagga gattattctc aaggagcatt ttatttatca
     1621 gcagatgcta taggagaaga tattagcata ttagacctta aagaagattc tcttcaaggg
     1681 gatagtgaag tagttgaaat tttatctagg atgggaatgg aaatattaag agaaggcaac
     1741 aaaataaaag gaataacaaa tggtttaaat ggtactttga tagatgcatc tcaatgccca
     1801 gatataatac ctgtattatc tgtagtagca tccttaagta tagggaagac tacaataatt
     1861 aatgctggta ggcttagaat taaagaatgt gatagacttc atgcaataaa tgttgagtta
     1921 agtaaacttg gagcaaatat agaggagaaa gaggatagtt tgataatcga aggagtaagc
     1981 aagttaaatg gtggagtaga ggtgtggagt cacaaagacc atagaattgc tatgacactt
     2041 gcaattgctt cttgtagatg tgataaacca ataatattaa aagattttga gtgtgtatca
     2101 aaatcttatc cacatttttt taaagatttt aagatgttag gaggtcgaat tgatgagtgg
     2161 aatatgggga aataatttaa aagtatcaat ttttggagaa tctcatggaa atgctatagg
     2221 tataaatata gatggtcttc cttcggggat tgaacttgac cttgataata ttgataaaga
     2281 aatgaagaga agagcacctg gaaaaaattc tatatctact tcaagaaatg aaagtgatat
     2341 accagaaatt ctaagtggat attttaatgg aagaactaca ggaacgccat tatgtgcaat
     2401 cataagaaat agtgatactc gttcaaaaga ttatggagag cttaaaaatt taatgagacc
     2461 aggacatgca gatttcacag gtaatgtgag atattctgga tttaatgact atagaggggg
     2521 ggggcatttt tcaggtagga taacagctcc attagtattt tgtggggcta tatgtaaaca
     2581 aattttatcg caaaaaggaa tagagatagg agcacacata aaaaaaatta aaaatataga
     2641 agatatgagt tttgattatg taaatatttc aaaacaacag ttatcaaatt tgcaaacttt
     2701 ggaactacct ttacttgatt tatcaaagga agaagccatg aaaaatatga ttatagatgc
     2761 aaaaaatcaa ggagattcag ttggtggtat aattgagtgt gcagtagtag gtattaatgt
     2821 agggcttggg aatccatttt ttgactctgt tgaatctact ttatcacatt tattgttttc
     2881 tgttcctgcc gtaaaaggtg tagagtttgg tttaggtttt gaacttgcag atatgtatgg
     2941 ctctcaaagt aatgatgaaa tgtattatga aggaaatcaa gttaagagca aaacaaataa
     3001 caatggagga attataggag ggattactac tggaatgcca ataattttca aagtggcaat
     3061 taagccaact ccatcaatta gcagacaaca aaacacagtt aatataaaag ataaaaagga
     3121 cgatatttta tatattaaag gcagacatga cccttgtata gttcaaaggg ctatacctgt
     3181 aatagaagca gtaacagcca ttggaatttt tgatttgatg aaaggacgat aataatggag
     3241 gatttaacga gatatagaaa tgagattgac aatatagata aagagcttat tcaattattt
     3301 gaaaagagaa tgaatacagt tcttgaaatt gccagacata aaatgaaaaa taacactact
     3361 atacttcaaa aagatagaga agaaaaagta ctaagtagag ccgtagataa tttagataat
     3421 aaagcatatt cacaagaaac ggtacagttt tttaattcta taatggaaat tagtagagga
     3481 ttacaaaaaa gattgataga taatgatggt gagcagaaaa tcgagtttag tttagattca
     3541 tcaaataaga attttgattt gtcaaatgta aataaatata agtctttaaa agatgaatta
     3601 aataaaaaaa atatcctagt aggatttcca ggaaaatcag gttcatttac agaagaagca
     3661 ctaaataaat tcttcaacaa aaaaatatca aaaaaacaat ttaaagaatt tgaagatgtt
     3721 ttcatagcac ttaaaaataa agaaataaac tatggaataa tccccataga aaattcatct
     3781 acaggagcta tctctgaaac ttatgacctt ttgagaaagt acggttttta tatagttgga
     3841 gaagaatgta taaaaataga ccaaaattta atagggataa aagacacaaa gcttgaagat
     3901 ataaaagaaa tatattctca tccacaaggt ataggacaat gtagtgaatt tttaaaacaa
     3961 aatagtacat ggaaattgat accatttaat aacacagcaa caagtgcaga acttgtaaga
     4021 aaacttcaag ataaaacaaa agcagcaatt gcaagcaaaa aagctgcaag tatttatgga
     4081 ttagaaataa taagtccttg tataaatgat atagcaaaca actatacaag atttgtagta
     4141 atcagtaatc aaatacacat tgaagaagaa tcaaacaaaa tgagtgtagt attttctgtt
     4201 gaacatgaag caggaaaact ttataaagta cttggatact ttgctaaaaa taatataaat
     4261 atgacaaaaa tagaatcaag acctatgaaa aatgcctcat ggagatattt cttttatata
     4321 gattttgagt gttctatata caatgctaaa gtgtacgatt tattagaatt gatagaacat
     4381 aatacagcat attttaaatt tatgggagta tataaaaata aaactgaata gataagataa
     4441 atttttattt aatttataat tattaggagg tgtaagtatg aattttttcg gtctagtagg
     4501 tgaaaaactc tctcacagtg tttcacctca aatccacaag agagtattcg agatattgaa
     4561 tattgaaagt gcttataaaa actttgaaat ttcaaaagag gatatatcta agctagacgg
     4621 tgctataaaa ttattaggaa tacaaggagt aaatgttact gtaccatata aggagcgtat
     4681 tatgaaatat cttgatttta tatcaccaga ggcaaaacgt ataggagcag ttaatacaat
     4741 tttacttaga gaaaatatgt tatatggtta caatacagat tactttggtc ttgatagtat
     4801 gtttaaaatg gctaatattg atgttcaagg aaaagtagca gttatattag gaactggagg
     4861 tgcttctaaa gctgctctta catattttat tgattctggt atagaaaagc tttatgtttc
     4921 aactagaaaa aaggatgata aaaaattatt aaatagtaaa gctatattaa tagattatga
     4981 agaacttaaa catataaaag gagatataat attaaatgct acaccagtag gtatgtaccc
     5041 taatgtagga atttcaccag tgtcaaaatc tattatacaa aattttgata tattaataga
     5101 tttaatttac aatcctggag aaacagagtt tttaagaatt ggtaatagta tgggtaaaaa
     5161 aacctgtgat ggattatata tgctggtagg gcaagcaata aaatctcagg aaatttggca
     5221 agatattaaa atagataata gtattttaga tgatatatat aatgaactaa aattagaatt
     5281 tttgtaaggg agattgttat gataaataaa acaaaagaaa aattgatatt aataggtatg
     5341 ccaggtagtg gaaagactac aattggtaaa cttttggcca aagaatataa ttgtagtttc
     5401 tgtgatatgg atgattatat aattcaaata tctcaaaaaa gtatagcaga gttgttttca
     5461 gaaggagaag atatttttag gaactatgag actcaagcat gtagagaact ttctataagt
     5521 gataaaacag tgatatctac aggtggaggt gtaataaaaa aggatgttaa tatggaaata
     5581 ttaaaagaaa caggtataat aatttttatt gatagaccta tacaaaagat tttggaagat
     5641 ataaatatta attcaagacc attattaaaa aacggaaaag atagattgta taatctttat
     5701 aatgagagaa taaatcttta taaaaaattt tcagatatag agattttaaa tgataaatca
     5761 ttaaacaatg ctatatataa tattacaaat gcagtcagtg aaaattttaa atttgagttt
     5821 aaagaaaaat aatctagggg gaagtaaaaa taatgaatat tgttattgta ggactaggag
     5881 ttataggagg ctcttttgct aaagcattaa aaaaagctgg atatgaaaat gtatttggag
     5941 tagatgtaga tttagaaact ttaaaaaaag cagaaaaagc aaaaatcata aaaaaaggat
     6001 gtacaacagg taaagagtta tttaaaaaag ctgatttaat tatactatct atatatccta
     6061 gattagttgt agattttcta aataataata aaaacttttt taaaaaagga actataatta
     6121 ctgatactac aggaataaaa gaaacactta tcaatgatgt gttgcaaatt attccagatg
     6181 atatagattt tatatttgga catccaatgg caggtaggga aaaaaagggg attgattttg
     6241 caagtgaaca agtttttaat ggcgcaaatt atataattac tccaactggc agaaacaaca
     6301 taaaaaattt agagttagtt gaaaatttaa ttttagagat tggatttaaa agagtaaaaa
     6361 agttaacttc acaaaaacat gatgagatta tagcttttac ttctcagtta cctcatgtta
     6421 tggcagttgc tcttataaat agtgatgaag aaggtagaga tacgggtaaa tttattggag
     6481 atagttatag agatttaact agaattgcaa atatgaatga agacttatgg agtgaacttt
     6541 ttttaggaaa tagagataat ttactaaaag ttatagaaaa ctttgaatca gaagtaaatt
     6601 tagtaaaaga agctatattt aacaatgata aaaataaatt gatagagtat tttaaaaaat
     6661 catcaataag aagagaagct ttagaaaaat aaatattaat atttaagcta catatagatg
     6721 ggtataaata taagcgatag tgtatttaat tgtattaata aatatggatt caatataatt
     6781 aaatatgcta ttgatatgtt tgtaccctta agtattgtta tcttttaaat gataatgata
     6841 tttatttaaa tgctctttat taacaattta taaaacttaa ttgtaaaaaa tacttattaa
     6901 tagtaaaata aagtgaatca attatattat aattgctaaa gagagtgagg tgtagctatt
     6961 tgaaaaaaaa tagattaata gcaaagaata tatcaatagc atttatagta ctattctttt
     7021 ttagtgtatt tacattcttt tatgtaggta atattaatag agtgttagaa tatgaaacaa
     7081 atgacataat aacagttaca attactggat ggattatact ttcattttta tttttaggta
     7141 ttataattta catattatat tcaaaagcaa agagtcaaaa aactatagaa aaagtagcat
     7201 atacggattt tgtaacaggt tattctaact ggagaaagtt tgaattagat gttacaaatt
     7261 tattaaagaa aacatcacaa aataataaat atgcaatggt catattcgat attgataaat
     7321 ttaaagcaat taatgatata tatggccata aaaaaggaaa tttaatatta aaagatattg
     7381 cagatacttt aaatgaattg actaatatca atgaaacttt tgctcgtgta agtgctgata
     7441 attttaatat attattaaca tataacaaaa aagaggatat aataaatatt atcaaaaaga
     7501 ttatggcaaa taatgaactt gtaaacttat cttttggaat ttatgaaatt aaagataagg
     7561 atttatctgt aagtgtatat agtgatagag cttcacttgc aaaatcatca ataaaaaata
     7621 atagtgatgt aaattttgca ttttttaatg ataaattaag agagaaatta ttacttgagg
     7681 ataagataga aaaagaaatg gaatatgctc ttgaaagtgg gcaattcgta atgtatctac
     7741 aaccaaaata caatattaaa ttagataaat tttgtggaag tgaagcccta gtcagatggc
     7801 aatatacaga aaaagaggtc atatatccag gtgattttat tcctatattt gaaaaaaatg
     7861 gatttattag aaaaattgat atgtacatat tagaacaagc ttgtaaagaa atccggtctt
     7921 tgtttgataa aggaatttca cctttaccta tatctgtaaa cttctcaaga gttgattttt
     7981 taaaaaaaga ttttattgca aacatagtaa atatatgtga tagatataaa ataccatata
     8041 gtttaataga aattgaaatt actgaatcta gtatgtttgg agatactgat actctattta
     8101 atgttagtag aaacctacag gatattggct ttatagttgc aatggatgat tttggctcag
     8161 gatattcttc tgtaaatatg cttaaaaata taccattaaa tgtaataaaa ttagatagag
     8221 gattttttgt tgatgataaa gatgttgata agagtcaaat tgttataaaa agcatagttt
     8281 ctctcattaa gcaattagga ataagggttg ttgctgaagg aattgaaaca agaagtcaaa
     8341 tagaaatgtt aaaaaaagca aattgtgata ttgttcaagg atattatttt tcaaaaccat
     8401 taccaattaa agaatttgaa aaattagtct ataaaatata gtcagcgagt cttaaatggg
     8461 aggtggtgat taagtgataa gagcaaaatc gttaaaaaat gtactgagta ttgttttaat
     8521 atttttattt acaataattt ctttttctga ttttgcattt aaaactaatg tccaagaaga
     8581 agaaaatagt aaaattaatt taagggaaat atccctacaa agttccaatg ctataaaaat
     8641 aaaattagat agttctctat cctttattaa aagtgtggca aatatggttt cttattttaa
     8701 tactatagat aactcaaaaa tagttccatt attagctaat atatcaaaag aatctgattt
     8761 tcaaagaatc tctattgtat ctcctgatgg aataagtcat accagcgatg gcaaaattat
     8821 caatgtgtca aattatcaat attttaagaa agctattaaa ggagaaagta atatatctga
     8881 agttgtaaag tctccaattg atggaaaaga tacttttgtt atagctactc ctgtatttaa
     8941 taagaataac aaaataattg gtgttttata cggttcttac tataatgata aattagctga
     9001 atttatagat ataacaagtt ttaatagtga agggtacata gatatatttg aatctagtgg
     9061 ccaatttgtt ttaaaatctt cacatacaaa caagctgatt aagtcaagta gtaatatata
     9121 tgatatattt aaaaaagctt cttttgatga tggttatagc tataaaaaaa tatatgaaga
     9181 tacaaaaaat aacaagagtg gctttacgtc atatgaatac aaaggagaaa atcgttatgc
     9241 tacttataca cctattggtg taaatgactg gtatgtaatt tctgtgatac cacaatatac
     9301 ggtaagaaga aacattgata aaataaatca attagcattt tctcttattt tcaaggtatt
     9361 cttattgttt attatactta taatatatat tatttataaa aacaataaat ctgagaaaga
     9421 tatactaaaa gctaacaaag aaatctcatc tattacaaat agcattcctg gaggagtaca
     9481 aaagtgtacc ctgggtgaaa catgcaatat taatttttta agtgatggat ttatagagtt
     9541 gtttggttat acaagagaag a
//
LOCUS       DAEORZ010000151         9498 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEORZ010000151 DAEORZ010000000
VERSION     DAEORZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.154"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            49..1809
                     /gene="prdR"
                     /locus_tag="KRH39_003197"
     CDS             49..1809
                     /gene="prdR"
                     /locus_tag="KRH39_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBG8513078.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            2238..4118
                     /gene="prdA"
                     /locus_tag="KRH39_003198"
     CDS             2238..4118
                     /gene="prdA"
                     /locus_tag="KRH39_003198"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBG8513079.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            4199..4483
                     /locus_tag="KRH39_003199"
     CDS             4199..4483
                     /locus_tag="KRH39_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513080.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            4521..5246
                     /gene="prdB"
                     /locus_tag="KRH39_003200"
     CDS             4521..5246
                     /gene="prdB"
                     /locus_tag="KRH39_003200"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4971..4973,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBG8513081.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            5326..6084
                     /gene="prdD"
                     /locus_tag="KRH39_003201"
     CDS             5326..6084
                     /gene="prdD"
                     /locus_tag="KRH39_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBG8513082.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            6099..6566
                     /locus_tag="KRH39_003202"
     CDS             6099..6566
                     /locus_tag="KRH39_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG8513083.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            6584..7066
                     /locus_tag="KRH39_003203"
     CDS             6584..7066
                     /locus_tag="KRH39_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG8513084.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            7086..8093
                     /locus_tag="KRH39_003204"
     CDS             7086..8093
                     /locus_tag="KRH39_003204"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBG8513085.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            8213..9094
                     /locus_tag="KRH39_003205"
     CDS             8213..9094
                     /locus_tag="KRH39_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG8513086.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         3730 a         1160 c         1890 g         2718 t
ORIGIN      
        1 tatatataaa taagttttga tgaggtacta ctataaaggg gggactttat gttttcgata
       61 ccagaagtaa aaaaagtaga agaggtaatg gatacaaaat ttacaactat agacgaagac
      121 acaagaattg aagatgcaat aaaagaaatg ataaaaagca acacaaaaac tctaatggta
      181 atagattcat ctgaccaatt aaaaggcatt atatccatga cggatataca taatctttat
      241 gagatgcata aaaagtatga aggtcaacca gtaaaactta taatgaagaa ggatgtaata
      301 tatgtcaacg aaggtttaac cttagatgaa tgtagagata taatgatatt aaaaaatata
      361 gggattcttc ctgttcttag agataataaa attattggag ttctaaaaca agagcacatt
      421 agggactatc tatatatgca tttagaagat tatggattga ctttgaagta tattataggt
      481 caaattaaag aaggaatttg tgctataaat aacgaaggtg ttgttatctt gtggaataag
      541 tttatggagg aaaggtatga tataaagtct gaagatatag ttggtcgacc gatgaatgaa
      601 tttttggaaa atactatttc agaaaaagta ctaaatagta aagttggaat gagtgacttg
      661 tattttacag ataaaaaaga aaatatgtat gcattagttc atgcaaatcc tatattttat
      721 aaagaagaat ttatcggtgt tgtatgtaca gaagttgatg ttacagaagc taagatatta
      781 gctcttgaac ttgaaaaagt aaatgataca ttaaaatatc taaagaacga agtcaaaaat
      841 ttatctaaag gtagttttga taagatttta ggtaaaagtt ataaattgga aaaatcgaaa
      901 gcgattgcaa aacaagtggc tagaacaaat agtagtatat ttatttgggg cgaaagtggt
      961 acaggtaaag aagtatttgc aagagctatt catgattata gtgagagaaa agggcaattt
     1021 atacctgtta actgtagtgc cataccaaat gagttatttg aaagtgagtt ttttgggtat
     1081 gaatcaggtg catttacagg agcaagtaaa aagggtcgaa taggtatatt tgaacttgca
     1141 aaagatggaa ctgtattttt agatgaaatt gccgatttac ctcttagtat gcaagcaaag
     1201 ttgcttagag tattacagga aaaagaaata agaagagttg gtggagatac cacaataaaa
     1261 ataaatccta gaataatatc agctaccaat aaagatttga gtaaaatggt aaaggcagaa
     1321 aaatttagag aagatttata ctatagatta aatgttgttg aaataaagat tcctccactt
     1381 agagagagaa aagaagacat aggattattg gttcatagct ttttagagga gatatgtaaa
     1441 caaaataata agcctgttct tacaattagc aaagatgtaa tagatatatt tcagaattat
     1501 agatggaaag gtaatattag agaattaaaa aatacgattg aaaatattgt tgtattgtca
     1561 caaaattcta aaatagatgt ggatgatgta ccaagttata tgatggattc tacaaataat
     1621 agtactgaag aagaagagta tcctttagat ttaacgaagg caactcaaaa aattgaaata
     1681 aagaatataa ccaaggcact taaaatgtct aatggaaata aagctaaagc agctaagata
     1741 ttaaatatac ccagaacaac tttgtattat aagatagacc aatacaaaat agatgtgtct
     1801 aaaatatgac acatgactaa attttgacaa aatgataaac tttaatttga aacctagtaa
     1861 agctaacgct tgtataagtg ttgtgtatat agatgtgtta aaaaaaatga aaaagtgttg
     1921 attaattgac actttttttg tttgagaaaa tgtttacaaa tacaaaaaat aacatatttt
     1981 aaataaatat ttatataata tttggctaaa aatagcgata gatgaagaat ctattataga
     2041 aaaaatcttt ttgttaataa gttggcatag gaattgctta taatataaag tgactttaag
     2101 tgagaaaaaa ataatattaa tcataacaaa ttatgtaggt tttaatttac ttaatttctc
     2161 agttaagggg ttacctgttg aggaattgag atttaaaata ataaataaga aaataatgta
     2221 aggaggtatt ttatactatg tcaataactt tagaaacagc tcaagcccat gcaaatgacc
     2281 cagcagtttg ttgttgtaga tttgaagcgg gaacaattat agcgccagaa aacttagaag
     2341 atccagcaat atttgcagac ttagaggatt ctggattatt aacaatacca gaaaatggat
     2401 taactatagg tcaagtacta ggagctaagt taaaagaaac tttagatgca ctttctccaa
     2461 tgactacaga taacgtagaa ggatacaaag caggagaggc taaagaagaa gtagtagaag
     2521 aaacagtaga agaagcagct ccagtatcag aagcagcagt agttccagta agcacaggag
     2581 ttgcaggtga aacagttaaa atacacatag gtgaaggtaa gaacataagc ttagagatac
     2641 ctttatcagt agctggtcaa gcaggagttg ctgctccagt agcaaacgtt gctgctccag
     2701 tggcaagtgc agcagcagaa gtagctccaa aagttgaaga aaagaaactt ttaagaagct
     2761 taactaaaaa acactttaaa atagataaag ttgaatttgc tgatgaaact aaaatagaag
     2821 gaactacttt atacatcaga aacgcagaag aaatatgtaa agaagctaat gaaactcaag
     2881 agttagttgt agatatgaag ttagaaataa taactcctga taaatatgaa acttacagtg
     2941 aagctgtatt agatatacaa ccaatcgcta ctaaagaaga aggcgaatta ggttcaggta
     3001 taactagagt tatagatgga gctgtaatgg tattaactgg tacagatgaa gatggagttc
     3061 aaataggtga atttggttct tcagaaggtg agttaaatac tactataatg tggggtagac
     3121 caggtgctgc tgacaaaggt gaaatattca tcaaaggtca agtaacaata aaagcaggaa
     3181 ctaacatgga aagaccagga cctttagctg ctcaccgtgc atttgactat gtaactcaag
     3241 aaataagaga agcattaaag aaagttgaca actctttagt agttgatgaa gaagtaatag
     3301 agcaatacag aagagaaggt aaaaagaaag ttgttgttat aaaagaaata atgggacaag
     3361 gtgcaatgca tgataaccta atattaccag ttgagccagt tggtacatta ggagctcaac
     3421 caaacgttga cttaggaaac atgccagttg tattatctcc acttgaagta ttagatggtg
     3481 gtatccatgc attaacttgt ataggacctg catcaaaaga aatgtcaaga cattactgga
     3541 gagagccatt agtaataaga gctatggaag acgaagaaat agatttagta ggtgttgtat
     3601 ttgttggttc tccacaagta aatgctgaga aattctatgt atctaagaga ttaggtatgt
     3661 tagttgaagc tatggaagtt gatggagctg tagtaactac tgaaggtttc ggaaacaacc
     3721 atatagattt cgcatctcac atagagcaaa taggtatgag aggtatacca gtagttggtg
     3781 taagtttctc agctgttcaa ggtgctctag ttgttggtaa taaatacatg actcacatgg
     3841 tagacaacaa taaatctaag caaggtatag agaatgaaat attatctaac aacactttag
     3901 ctccagaaga tgctgttaga ataatggcta tgcttaaaaa tgctatagaa ggtgtagaag
     3961 ttaaagctcc tgaaagaaaa tggaatccaa atgttaaatt aaataacata gaagctatag
     4021 aaaaagttac aggagaaaaa atagtattag aagagaatga gcaatctcta ccaatgagta
     4081 agaagagaag agaaatatac gaaaaagacg aaaactaaaa tttagatact aactaagatt
     4141 tagacaataa cataaattta gatccaagtt taaaacagta tagagaggta gaattataat
     4201 ggataggtta aaatatatct catcagagac tttttatgaa ggtattatag ttgacatcaa
     4261 aggcggtgga gtgactatag acctaaaagg tagacttgga caatttaaaa tacctaatag
     4321 aatgcttata actgactacg aacttaagat aggtcaagaa gttggattca tgctaagcta
     4381 tcctgaggta ttaagtccag agcctaatca agagtatgta gaaaatatta ggagagaaaa
     4441 agaaaaacaa gcagaaatgc aatcaaaaca agaagaaaaa taataaataa tcgtttgaaa
     4501 atagaaggga gaggaaatat atgagcctta caacagtaca aggacttcaa tctgaaatat
     4561 tcgttccaat aacacctcct ccagtttgga ctcctgtaac taaagaatta aaagatatga
     4621 ctatagcttt agctacagca gctggtgttc atttaaaatc tgacaagaga tttaatctag
     4681 caggagatac tacatttaga gctataccta atacagctac agttgatgaa atgatggtat
     4741 cacatggtgg atatgacaat ggagatgtaa acaaagatat aaactgtatg ttccctatag
     4801 atagattaca tgaattagct gcagaaggat ttatcaaagg agtagctcct atgcactatg
     4861 cattcatggg tggtggagga aaccaacatg tcttcactga agaaactggt cctgctatcg
     4921 ctgctaaact taaggaagag ggagtagacg gtgtagttat gacagctggc tgaggtactt
     4981 gccatagaac tgccgtgatc gtgcagagag caatagagga agctggaata cctacaataa
     5041 taatagcagc tcttcctcca gtagttagac aaaacggaac tcctagagca gttgctccat
     5101 tagttccaat gggtgctaat gctggtgaac cacataatat agaaatgcaa acacatatat
     5161 taagagatac attagagcaa ttagttgcaa taccatctgc tggtaagata gttccattac
     5221 catacgaata taaagctcac gtttaaaatg taatttatat ttgaaaatat agaacccttc
     5281 tcctacacgg ttaggagagg ggttttaaga acaaaggtgg atattatgga agaaaaaata
     5341 atgagaagac ttgttataaa accgtttcat atgaatgaag ttaatttcgg ttcaaaaact
     5401 tctataaaga aagatgtact tacaatagat ttatcaagta tagatgaaat aaaagaaaga
     5461 gaagagttaa taacagacat aaaagtagac attataaaac ctggagatta tgacagagaa
     5521 attaatacta ttatggacat tataccaata tcaacaaaag ttttaggcag actgggagag
     5581 ggtattacac atactttaac gggtgtatat gtaatgttaa ctggtgcaga tgaagatgga
     5641 aatcaaatgc atgaatttgg ttcttcagaa ggaattttga aagaccaaat ggtatttggg
     5701 agatatggaa ctccatcagt tgaagattat ataatccatg tagatgtaac tttaaaggga
     5761 ggtttacctt ttgaaagaac acttccactt gcagcattca gagcatgtga tgattttata
     5821 caagaaataa gagcgtcttt gaaaatggaa gacggaagaa atgctactca agttcgtgaa
     5881 tattttgaca aaattcgacc aaatgcaaaa aaggttgtca tagtaaaaca aatagcaggt
     5941 caaggtgcta tgtatgacaa tcagttattc tcaaaggaac ctagtggatt tgaaggagga
     6001 acatcaatta tagatatggg aaatgtacct atgattatat ctcctaatga atacagagat
     6061 ggtgccctta gagctatgac ttaagagagg tggatattat gggaatagga ccttcaacaa
     6121 aagaaacatc tcttcatcac tttagagacc cacttttaga catagttgaa agtgataagg
     6181 atgtagattt acttggagta atagtagtag gaactccaga tggaaatgaa aataaaacct
     6241 ttgtgggaca aagaacggcc gcttggctgg aagccatgag agtagatgga gctatagttt
     6301 catcagatgg ttggggaaac tcacatgttg actacgctaa cacatttgag gaaataggaa
     6361 aaagagatat acctgtagtt ggagttacat ttaatgggac tcaagctaag tttgttgtaa
     6421 gcaatcaata tatggatacc atagttgata tgaacaaatc aaaagaaggt attgagacag
     6481 aagtagttgg agaaaacaac acaaatgaaa tagatgctaa aaaagctttg gcatttttaa
     6541 aacttaaaat gagaaaacat ggataaaaaa tttaagtaag ggaatgagtt ttgtgggaat
     6601 ttctacatca atgaaaaaca catcacttca tatatttaga gacccgttac tagatttgat
     6661 agataatgat tctgacatag acttgttagg agtggttgta gttgggacat cagagcacaa
     6721 tgaatggaaa acatttttag caactagact tggaagatgg attgaatcat tgcgacctga
     6781 tggagtgatt ataactttag atatggcagg aaaccagcat attgatttta caaatgctat
     6841 agcagagttt gtaaaatcag atatacctac agtaggtctt actattatgg gagcagatgg
     6901 attagtaatt accaatccat atttggataa agctaccata attgattata aaaagacaac
     6961 aggctacata gaaacagaag tagttggaga caatcacatg gacgaagttg atgttaaaaa
     7021 agcattggct ttcctaaaat taaaaatgcg taaagacgca aaataaacct aagggggaat
     7081 ttgatatgaa atttagcaga agtatacaag ctatagactc tcatacagca ggtgaagcaa
     7141 ctagaatagt tgtaggtgga atacctaaca taaaaggaaa ttctatgcct gagaaaaaag
     7201 aatatttaga ggaaaatcta gattatttaa gaactgctat aatgttagag ccaagaggac
     7261 ataatgatat gtttggttct gtaatgactc aaccttgttg cccagatgct gactttggaa
     7321 taatcttcat ggatggtggc ggatacctta acatgtgtgg ccatggtaca ataggagcta
     7381 tgacagcagc tatagaaaca ggtgtagttc cagcagtaga gcctgtaact catgtagtta
     7441 tggaagctcc agcaggaata ataagaggag acgttacagt tgtagatgga aaagctaaag
     7501 aagtatcatt cttaaacgta ccagctttct tatataaaga aggtgttgaa gttgacttac
     7561 caggtgttgg aactgttaaa tttgacatat catttggtgg aagcttcttt gctataatac
     7621 atgcaagtca attaggttta aaaatcgaac ctcaaaatgc tggtaaatta actgaactag
     7681 ctatgaaact tagagatata ataaatgaga aaatagaaat acaacatcca actttagctc
     7741 atataaaaac tgtagactta gttgaaatat atgatgagcc aactcatcca gaagctactt
     7801 acaaaaatgt agttatattt ggtcaaggtc aagttgacag atctccatgt ggaactggaa
     7861 caagtgctaa attagctact ttacatgcta aaggtgaatt aaaagtagga gaaaaattcg
     7921 tatatgaaag tatattagga actttattca aaggtgaaat agttgaagaa actaaagttg
     7981 cagatttcaa tgctgtagta cctaaaataa ctggttctgc ttatataact ggatttaatc
     8041 attttgtaat agatgaagaa gacccactta aacatggatt tattcttaaa taagtaataa
     8101 ataaattagg aaattaaaat agaatagtag ccacttgtcg gttgctattc tatagctttt
     8161 aggctataag attcagaata tatgaaaaaa taaaaaaata ggaggatgga ttatggtagc
     8221 aatcttaaag gcattatatg tagtattcgc agcaatcttt gcagttgtgt ttggtaaaga
     8281 cgtagctaaa gcaaacaaag aggggaaact agaagaacaa agtactccaa aagttgctgt
     8341 aacaggattt ataacagact tctttgatac attaggaata gggtcatttg ctccaacaat
     8401 agcaatgtca aaggctttaa agttaaatat accagataaa aaaatgcctg gtacattaaa
     8461 cgtagcacat actattccag ttgttacaga agcattcata tttacatcaa tcataccagt
     8521 tgatggtgtt actttagtat ctatggttgt ggcagcagca gttggttcat atataggtgc
     8581 aggaataata gctaagatgg acgagagaaa aatacaactt gttatgggtg taactttagc
     8641 attaacagca atattaatgt tattaggtca tccttggatt aacgtattac caggtggagg
     8701 aaatgaatta ggtcttactg gtgttaaatt agtaataggt gtaataggta actttatact
     8761 aggagcttta atgacagctg gtataggtct atatgctcca ggtatggcaa tggtttattt
     8821 cttaggaatg tctgcaaaag ttgcattccc aatagttatg ggttcttgtg cattattaat
     8881 gccagttgca agtatgaaat tcataaaaga agatgcatat actaaaaaag catcagttat
     8941 aatagctata tgtggtttaa taggagtttt cgtagctgca tatatagtaa aaactctacc
     9001 aatggatata ttaaaagcat tagtaatagt agttatagct tatacttctg ctacaatgat
     9061 aatggctgca aataaaaaca aaaagactgc ttagttaaag actacttagt gtttattaaa
     9121 tattacagct aataataaag attataaact gctttaaaat attgaattaa tattttaggg
     9181 cagtttttta tttactaaat taatataaag caattaacat gccaattatg taatatatat
     9241 agtgatttag tagatttaat gtaaaaaacg ttgtattttt aaagaataaa gcataattta
     9301 aagctaaata tttaaatttt ataatcaata agttcaataa atgttcatat attaatacag
     9361 attaataaaa atataacctg taataagtct aatataactt ataattaaga aaaataagtt
     9421 tataatgact caatattcat ctgaattatt tgaataaatt taattcgttg aagttgctta
     9481 tatagagtga ataaattt
//
LOCUS       DAEORZ010000152         9004 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEORZ010000152 DAEORZ010000000
VERSION     DAEORZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9004
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.155"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(89..697)
                     /locus_tag="KRH39_003206"
     CDS             complement(89..697)
                     /locus_tag="KRH39_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBG8513087.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(806..3544)
                     /locus_tag="KRH39_003207"
     CDS             complement(806..3544)
                     /locus_tag="KRH39_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG8513088.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINVCISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKNYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3692..4483)
                     /locus_tag="KRH39_003208"
     CDS             complement(3692..4483)
                     /locus_tag="KRH39_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG8513089.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4519..5175)
                     /locus_tag="KRH39_003209"
     CDS             complement(4519..5175)
                     /locus_tag="KRH39_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513090.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5168..6133)
                     /locus_tag="KRH39_003210"
     CDS             complement(5168..6133)
                     /locus_tag="KRH39_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBG8513091.1"
                     /translation="MISIKNVNKYYGKVQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGNVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERRTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6239..6345)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6634..7577)
                     /locus_tag="KRH39_003211"
                     /pseudo
     CDS             complement(6634..7577)
                     /locus_tag="KRH39_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
     gene            7688..8155
                     /locus_tag="KRH39_003212"
     CDS             7688..8155
                     /locus_tag="KRH39_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513092.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTVMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8323..8922)
                     /locus_tag="KRH39_003213"
     CDS             complement(8323..8922)
                     /locus_tag="KRH39_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513093.1"
                     /translation="MKLKRTLFVGLALVLAIGITACARDNEKPNENAGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKRMNDDLIAEGERLVKDIDERIEKLDNVSDEDMKKGQN
                     DFMKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         2916 a         1501 c          876 g         3711 t
ORIGIN      
        1 ttttatcaaa ctaaaaattc tataaaaaaa taagatacat acctataaaa taccttccta
       61 tagatacata tcttacctat ataaaaactt attctatata aaaacgccat aaaaaatctt
      121 ttgcttcttc tgcataatct atatttatac gtttactagt ttttatatct aactcatctt
      181 tactaaatac tttttttcct ttatcatcat aataaaaatc acttatatat aattcttcac
      241 ccattatact cttactattt agacttctat ctatttttaa agctttgcaa agttttcctg
      301 gtccgttagt aatatttttt acttgatatt tactcaactc agtgtaagct ttgttgtatc
      361 tattcataga tatctcatct aaagaagtta taggctctac ccctcttatt aatacacact
      421 ctggcacatt ttctatattt gcagataaat tcaagcaatt atacatacca taaattaaat
      481 aaacatatat atgtccacca tctaaatata atggcttagt tctatctgtc tttttattgc
      541 catatacatg tgctccttta tcatttacac ccatgtatgc ttctgtctct atgattttgg
      601 taacaattac tttattttca tatttcctta ttagatattt acctaatatt gatttggcta
      661 aatcaattcc attttttcta aaaaaatcct tttccaaaat ttcacctcat aaaatttatt
      721 taaaacgcat caaatacaac taaagtaata aataaaatta actcttatta atgtgatata
      781 tataaacaat aaatttagac atttattatt tatatatatc aaaaaattct atctcaaact
      841 tttcaatatt atcattgtaa tttgaattat catatacttc ttcaacaacc tctttaattg
      901 ggaatataac ttttacatta gttccttctc ctaccttact atctatgtaa atcttcccat
      961 tttgaatttc aatcaatgat tttgtaaggc ataaacctat accacttcca aattcatttg
     1021 atataccact gttttcaatt tgtttaaatc gattaaatat atcatttaat ttttctttgt
     1081 ctatgcccac tccagtatct tttataatta tctctactgt ttcgtctttt ttatatatac
     1141 ctacaaggat ttctcctcct tctaaagtaa attttatagc atttgataat atatttagta
     1201 ttattctttc tatcgattct gggtcaagcc ctaccataac agtttcttca tctgtatcaa
     1261 aaattaaatc tattccttta gacttagcat aactactaat agatgtaact attctttctg
     1321 ttatgtcaat aatatcaaaa ttaactgctt taatcttata tatgtctgac ttaacttgag
     1381 aaatatttat tatattatct attaatctca ataatctaaa catattttgt ttagttattt
     1441 ttatatgttc ttttagtata tcctgaaaat caattatttt tcctttttta taaaggtttt
     1501 caataagttg aactgaacta tatatattat ttaaaggtgt cttaagttca tgagatatat
     1561 tagcataaaa ctccatcttc atttggtcat actgtctttg ttttattaat gcttttttaa
     1621 gtctttcaga ctctctttgc acacttaaat ctttaataat tctcactcta cttcttttct
     1681 gtccaaagta taatgttctt gatatcattt ttacttcttt aatgtgatta ttttcattta
     1741 ctatatggat actcttctct ctttctccta tatttaaatt gtttatgtat ttttttacta
     1801 atatatttct atatttttgt gttgtaactc ctaatacatc actagaaatt ccgaacaact
     1861 ctctaaaggc tctatttgta tatagtactc ttgatgtact taaatcctca agaaaaatag
     1921 cctctggtat aacatccagt aatttttcat atattatttt ttgttcattt actgttctaa
     1981 gaatatttat ctctttagtt atatctctaa atgtaagcaa tttaaagagt ttattatctg
     2041 accaatagtc tccaactcta acttcttcaa catgttcttc accttctgag tcagacatca
     2101 caaaattaac tatacagttt gctgtgtcat tgtaacttac tatttcagct ttcttttctt
     2161 gtgttagatt taatctttta aattccctat tgtcaaagaa atctatgtta aaagtttcaa
     2221 aatattgttc taatgtcata tcgactaaat cacttgaaac acatttaaaa ctcttttcat
     2281 attttttatt tatacttatt accttattct tctttatacc accttcagca cttcttacaa
     2341 gtataggaga tggaatattt agtataatgt tttcatagtt tttatttata tttcttattt
     2401 cttcatctat tttgatttca tttgtaatat catttaaact tattaagtaa ttgctttcat
     2461 ctttattatt acttaaactt aacaaataaa ttttatcatt taactctacc agtttatcaa
     2521 ttatacttat atcgttatca aatttcttca atttttcagc aactttttca tcttccttta
     2581 taaaagttaa tattttataa taattattca tatcctttga aaattcatct ttaaatttat
     2641 tatttgtgta acttatatta aaatcatagt ctgttactat ccatacattt ggtattgtat
     2701 ccagagcttg tctaagtaat tcttttctct ggtctatgta gttttttaat gttctttgta
     2761 taatcatatc cttttctatt gctaaattta tctttcctaa ttgctctgac tttttactta
     2821 attttttatt gattgaatca agttcataat atggatacac tactctatta ttaagactgt
     2881 atacatatac ataataagta tggttacata atagtaaata tattactcct attaatctat
     2941 ctgaatctac tatattaatc ttagaaattg ctacaaaaca tataatagtt gtaaaaaaaa
     3001 caaaatatgt taaatctttt attaacttat agtgctcaat gttactatca agtttattca
     3061 acaaatatag taaattaaac atagatatac aaacattaat taatataata atcatattcc
     3121 agttaatatc actaaaaata tttcttataa agtaatctaa tatcacaaaa gcactataaa
     3181 aaattagtat tgggcttatt cttagttttt ttactttaga aatggttaag tatataaaag
     3241 tagacatatt aattatgctt aagttaagca atacaatttg gaatttttga atttttaaat
     3301 ttactactcc taaaatactt acaaaaacta atacaagaca ccctactttc aatatattta
     3361 taaattcatc tgttttaaag ctacttatat aaactataga atatccaaat atccaaagta
     3421 aaattattct atacatattt atagaaactg tatacaaatt tatatttaaa agtgcaataa
     3481 ccaaaaataa cccattagct agtatataga ccttattctt acttaaagtt cctttagatt
     3541 ccatattccc caccacttct tcatttttat atagttttac ctataaatat gttaccacaa
     3601 ttcttccaaa aaatttgtca ctttctagtt ataaaaaaaa atgagtaaat atttttaatt
     3661 ttactcattt tgtagatttt gtatattcag tttaaaaagc tggaactata cttcctttat
     3721 attcatcttg tataaatttt ttaacttctt tactattcat agcctctgat aaagccttaa
     3781 tcttatcact atctttatta ttttctctac aagctattat atttacatat ggagaatcac
     3841 ttgattctat tactattgca tccttagtag gatttaagtt agctgttaaa gcataattag
     3901 aatttattac tgctccgtca acatctttta atactgttgg taattgctct gcattcattt
     3961 cttttattac tatattttta ggatttttag ttatatcttt tttagttata agctctccat
     4021 cttttacttc tatcaattta ttttttgcta ataatttaag tgctctagct ccattagttg
     4081 catcatttgg cacagctata acagctccat cttttaattc atctaatgac ttaacttttt
     4141 ctgaatagaa tcccattggc tctatatgaa ctttagatgt ataagtaagt ttatatcctt
     4201 tctccttcac agtttcctct aaaaatggaa tatgttgaaa gaagttagca tccaaagacc
     4261 cttcatctaa agctgtattt ggaagtacat aatcatcaaa tatttttact tctaaatcat
     4321 atcctttttc ttttaacaaa ggttttgcaa cttccaatat ctttgcatgt ggatttgatg
     4381 aagctcctac tagtattttt ttatcctctt tgtttgaaca acctactgct gatattgcta
     4441 ttgctgacac caatgctact gataataatt tctttaattt catttttact ccccctcttt
     4501 aaaatgttta taattaattt attttagttt cttataagcc agatttccta ctccctggat
     4561 tatctgaact aatattataa gtgctattac agtatatacc attactgctg tatcaaatct
     4621 ttgatatcca taaataagtg ctatattacc taatcctcca gctcctactg cacctgccat
     4681 agctgtataa ccaagtatag atattattga taatgtaatt ccagatacta ttgaaggcat
     4741 agcttccttt atcataactt taaatactat ttgtcttttt gtagctccaa aagattttgc
     4801 tgcttctatc aatcctttat caacttcatt taatgagctt tctataattc tagcgataaa
     4861 cggtgctgcc ccaatagtta ttggcactat tgcagcagtt tcccctatac ttgttccaac
     4921 gattaatcta gttattggaa tcattgcaac tataagtatc ataaatggga aacttctaaa
     4981 tatatttact ataaatccta atgcactatt tattgtgcta tttggtttta atccatctgg
     5041 ttttgtaact actaatatga ttgctaatat aaatcctaat attgttgcca ctatagttgg
     5101 tactataacc atatacaaag tttgtaacaa tgcatttgga aatagtgttg ttaaaaagtc
     5161 aattaaacta ttcatttatc atttcctccc atcttatgcc cttactagtt agatattgtt
     5221 ttacttgctc tccactttta tctgaaatgt ttataataag tgaacctaaa atatcatctt
     5281 taaattgttc taatttccca aatattatag acacatctat atttagttct cttgccatag
     5341 tcgtaataat agcttcatta gaaatatctt tagggaatag tatctttata tttgttcctt
     5401 ttggtaatat tatactttct tcacctatta attttctaag tcctttagta tttcttaaga
     5461 atatttcttc tgtatctcct acctctaata cttctccatt ttccattatt gctactcttc
     5521 cacaaatctg ctttataact tccatttgat gagttacaac tattattgtt attcccaact
     5581 ttttatttat atcctcaagc aatgaaagaa ttgacttcgt agtatttggg tctaatgctg
     5641 aagttgcttc atcacaaaga agtacctgtg gatttagtgc taaagctcta gcaattgcaa
     5701 ccctttgttt ttgtccgcca cttagatttc tgggtttatc atttttcttt tcagatattc
     5761 caactacttc taataactct aaaactcttt ttttaatttc tgcctttgag tatccaaaac
     5821 attctaatgg caatgctaca ttgtcaaata cagttcttct ttcaagcaat gaaaagtgtt
     5881 gaaaaatcat tcctaactct tttcttaaat ctctcatttg cttttcattc aatgacttta
     5941 cttctttatc agaaacaagt acattgcctt cttggtattc ttctaatccg tttatacatc
     6001 taagtagtgt tgattttcct gcaccactat gacctattat tccaaaaatt tctccagatt
     6061 ctatttcaat acttacatct ttaagcactt gaacctttcc ataatactta ttaacatttt
     6121 ttatgctaat cataaaattt cctcccatat attctgctca gtttttatgt ttatcctctc
     6181 ttatgtatta caacaaaata aaagcccgag tataaactca ggcttaaaaa gctatttata
     6241 ctcatctatc aactatatta gttgcaggat ttagcaccaa tgtatataaa atatactggt
     6301 tgccgggttt caaagggcca gtccctccac cactcttgat aagacgttat ttaatttaat
     6361 tctattatca tttgttgcta tgttatatat gatatattca ttaatagtat ttgtcaataa
     6421 atttatttaa attttttatt ttttattatc gttaattaac tatttttaat tagttttcta
     6481 gctcctaaca ctttatattt taatgtctat ataactatag aacatcatta taagaaatat
     6541 attaaataat atcaatctat ctaaatcatc tgtaaagtaa attaagaaaa ttaacatttt
     6601 ctccaaactc tatcctatct taaataaaat aatttaaata caatgatgat tatatagctc
     6661 taatacttta gactttatcg aatgtttaag ttctataggt tctattattg tagcattttt
     6721 ccaaaagtta aaaaatattc agccacccaa ttaagtgtag attttggtat atacatatct
     6781 atgtatccac ccttagacaa aattttcaat cctcttgcca acaaaaattc tgtttcacaa
     6841 cgttttactc cctcatctgt cagattaatt ttaatatgat aatctgtacc tacttccata
     6901 ttcttaagat aatcatgtat agaagataaa accttatatt tcttatttga taagttttct
     6961 atactcaaaa tcttcacaat tctgtctgca cgaaattcct taacagattc tgattttata
     7021 cagtaagctg gacaatacca aagtccattc atagcataca aaccaatagg aacaatggta
     7081 cgtatactat ttttttgctt tgatgaatat tcaattttaa ttgtaaattc attcattact
     7141 atattaaaca attccttcaa taatggtgtt gagcaatgcc tatctggtgt ccaaaaaaca
     7201 agtttacgtc tcatattctc aatattatat tgaatatcat ttggtataca atttaaaaat
     7261 ttctttaaaa cagaaatagt ttcttgctca aatggtaaat cattataata ctctagtgac
     7321 tgatatgcaa agaatataga ttttacctca ctctctgaaa atgtaattgg tggaagtata
     7381 cgttctttaa gtacatgata tcctccagcc gctccaactt ttgaatatag tggaaatcca
     7441 acactttcca atacctgtaa atctcttagt atagtacgtt ttgatacaga aaattcttct
     7501 gctagctctc ccgctgtaaa acttcttttt gcatttattg ttatcatcag ttcaattaaa
     7561 cgctcagctc ttgacatatt catactccta atttaaatat agtgacaata attgtcacca
     7621 ttatatgata agatattctt gcatctaaag caataataat attaaagaaa gaagggattg
     7681 atattttatg gaaaatgata ctattaaggc tgatgatatt ctcaattatt gtctatcaaa
     7741 cttagatgat gttgtactaa tggatagttg gggggaacga gcaatttact acaatcctaa
     7801 tggtgtttta aagcgagggg tatatgttct taccattaag gaaaaagaca gtaataatga
     7861 taaaggttcg ttagttagtc gtccaaatgt ataccgtgtg aatataggat taaaaaaaga
     7921 aacttttatt gaaatgtttg gatatattcc aaagcgtcca ggtgtagggc aaatagttga
     7981 tatggatttt gattttacaa aattggacac agtcatgcca catcctatct actcatggat
     8041 gggatggata tgcgttctaa gccctactga aaagacattt gagaacttta aaactttaat
     8101 aggagaatct tacaattttg caaaacaaaa atttaaaaag agaaaaaaca agtaaaaaca
     8161 taaaaaaata acctctaaat aaactataat gaaaatatga ggttacaaga taaaaattag
     8221 tatcttgata acatacaaaa aagcgaagcc aaaaaatata ttaattacaa ggcttcgcta
     8281 tatttcaaat atttctttct aactttaaaa ctatttactc tattattttt tattatttcc
     8341 tgttattccc aatctatcat tcatatcttt tatcattttt ctaaaaccac taccaatatc
     8401 gtttccaaca tcttcagttc catgaactag tttcataaaa tcattttgac cttttttcat
     8461 atcttcatca gatacattat ctaatttttc tattctctca tctatatctt ttactaatct
     8521 ttccccttca gcgattaggt catcattcat tctctttaac tctgtatctt ctgttttacc
     8581 gtctttttta agagatgtta caaatgattg tattttatct ctactatctt tatatgcagc
     8641 ctttacatca cttagatatt tttcatttcc tggatattct ttatctttaa actgattgtt
     8701 tatactttct ggagtatcat aaatagaata tctatttaga cctgctatat aatcattata
     8761 acttgttgaa taataattca tatatccttc attattattt acagattcat taggattttt
     8821 tcctgcattt tcatttggtt tttcattatc tctagcacat gcagttattc ctatagctaa
     8881 tactaatgct aatcctacaa ataaagttct ttttaatttc atttttatta ccctcctaga
     8941 tgttttgtac tacaaaaaat tttaataatt ttctttctta atttagtatt tctatatttt
     9001 tatt
//
LOCUS       DAEORZ010000153         8991 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEORZ010000153 DAEORZ010000000
VERSION     DAEORZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8991
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.156"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(261..824)
                     /locus_tag="KRH39_003214"
     CDS             complement(261..824)
                     /locus_tag="KRH39_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513094.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(983..1696)
                     /locus_tag="KRH39_003215"
     CDS             complement(983..1696)
                     /locus_tag="KRH39_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBG8513095.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1988..3652
                     /locus_tag="KRH39_003216"
     CDS             1988..3652
                     /locus_tag="KRH39_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBG8513096.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(3759..4658)
                     /locus_tag="KRH39_003217"
     CDS             complement(3759..4658)
                     /locus_tag="KRH39_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBG8513097.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(4685..5323)
                     /locus_tag="KRH39_003218"
     CDS             complement(4685..5323)
                     /locus_tag="KRH39_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG8513098.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(5660..6658)
                     /locus_tag="KRH39_003219"
     CDS             complement(5660..6658)
                     /locus_tag="KRH39_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBG8513099.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(6756..7331)
                     /locus_tag="KRH39_003220"
     CDS             complement(6756..7331)
                     /locus_tag="KRH39_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513100.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            7647..7910
                     /locus_tag="KRH39_003221"
     CDS             7647..7910
                     /locus_tag="KRH39_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513101.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(8347..8724)
                     /locus_tag="KRH39_003222"
     CDS             complement(8347..8724)
                     /locus_tag="KRH39_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8513102.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT         3177 a         1365 c          938 g         3511 t
ORIGIN      
        1 ttatacgtgt atgtataaat actttttata cactgtttgt actatccatc ccgcacctaa
       61 aggagtggga tttttgcact attctgtatt atatatatcc aaaaatatta taaaaataca
      121 aatattagca gaaatttaaa aactgaagaa agtattctaa ctaagattta caaattctta
      181 atcctatcga agttaaacta ctatcttcag cctaattatc tagcaatatt tttttatatg
      241 aaaaactcta aatcaagttt ttaaatgttt gcgatttttg atacataaga atgttgtcta
      301 gaattatatt ctgataataa accttcatac tctaaggtta agccagataa attataaaaa
      361 tcatttggcg ttacttcaaa tgcttgttta tttaaaatgt ctatctcact tttttctaac
      421 atgtcagcaa caaaatttga acatacatat ttatattcag attgtctctg aatattcatt
      481 agtaaatata ttaatgccat tgtatcgtaa tagtaatctt ctctataatc actaattaat
      541 tttatatttt tatatagatt ttcatattgt aaattatcta cttctaacga atatacccta
      601 cacacagtgt tttttcttat agcatataat ccttgattta tattttcttc aacaaatcct
      661 ccaataaatg gattccttgg atttagcctt ccaaaagaat acattggttt tagattttca
      721 tttaatgata ttgacacatg tgtatacagc tttttactta tattccttat taagtaagat
      781 aatatagttc ctgtatatgt tgttacaata tagactttat tcatcatttc cccccaatct
      841 agtaactaaa taatattata cacttacaat ttaatatcat aagatattct aacataataa
      901 atgtggtttt acaaattttg attttaaggt actttaatat tatataatat aataaaaata
      961 taaatataag ttttctatat ttttaaccaa cagatgtttt cttcaaatta atcagatgat
     1021 ttagtatatc ttgaaccttt ccagagtcct ttaaatatcc taatgcccca tttatttgtg
     1081 attttaaatc tatttctact tccatatcca aatcaacatt tccttttttt atagatatat
     1141 ttttaagtgt tattgggtaa agataacttt tttccaaagt aatcttattt ccattaacct
     1201 caaatggaac ttttttattg tagttactta atattttttc tagcatttta tcacttagtt
     1261 taaattttcc aattttcaca ttctcaagac ttacaactaa gtttccatcc tttaaagtag
     1321 gtctcaattc aagttcatat tgactattta ccaaaccaaa taccttatat ggtcctgcta
     1381 cctttatttt accatttttc atttcaacaa aattattttc aagttcatta attccatgct
     1441 tctgcatcaa tgtatatatt aaatttttaa actcttcctc tgaaacagat atttttccaa
     1501 ccattctaag tggatttttt actacctcca tagaatttat atatgagcct tttgataaat
     1561 attcttcatc tatgatttta ttattttgtg aactaacatc atatctttct tttggtgtca
     1621 agatatatac aacaattcct gcaagtacta aaacaataac aattaaactt aacaaaatct
     1681 ttgcaacctt tcccataatc gtctccttta caaatctgtc atcagattta aaataaattt
     1741 agataactaa cttcacataa tattcttaac aatatacata ctataataca gtcttttaaa
     1801 aattaattca accaatctaa tgttttgtaa ataaactgta aaaacatgtt tttagtataa
     1861 aagttaatat tttgcataat actcttaatt acacctttat ttttttctaa tcttggatta
     1921 aaattaatat tatgcaatac agtgtagtgt tctgtattat aatcaaatta aataggaggt
     1981 atagaatatg tcaaatgaaa cgaactcatc taactttata aagaatatta ttataaacga
     2041 cttagaaaca ggaaaacatg acagtatcat aactcgtttt ccacctgagc caaacggata
     2101 tttacatatt ggtcatgcaa aaagtatatg tcttaatttt ggattagcta aggagtttaa
     2161 tggaaaagct aatttaagat ttgatgatac aaacccatta aaagaggatg ttgaatatgt
     2221 agagtccata aaagaagatg taaagtggtt aggatttgat tggaacgagt taaattttgc
     2281 atctaactac tttgatgaaa tgtataagag agctttaata ctaataaaaa agggtaaagc
     2341 ttatgtatgt gatttgacac aagaggaaat gagagaatat cgtggtactc taactgaacc
     2401 aggaaaagaa agtcctcata gaaacagaac tattgaagaa aaccttgatt tatttgaaag
     2461 aatgaaaaat ggtgaattta aagatggtga aaaaacatta agagccaaaa tagatatgtc
     2521 ttctccaaat ataaacctta gagacccaat tatatataga atatctcatt caacacacca
     2581 taatacagga gataaatggt gcatatatcc tatgtatgct tttgctcatc caattgaaga
     2641 tgcaattgaa ggaataactc actctatatg tactttagag tttgaagacc aaagacctct
     2701 ttatgattgg tttgttaaag aatgtgagat ggaaaatata cctagacaaa tagaatttgc
     2761 tagacttaat ataaataata ctgtcatgag taaaaggaaa ttaaaacaac ttgtagatga
     2821 gggtattgtt gatggttggg atgaccctcg tgttcctaca atatccggaa tcagacgtaa
     2881 gggatatact gcagaagcat tgcgcaattt ctgtagtgaa ataggggtat caaaagttaa
     2941 ctctacggta gatagtcaaa tgcttgatta tttcctaaga gaaaacttac aaccaaaggc
     3001 tcctcttact atgggagttt taagaccttt aaaacttatt ataactaatt acccagaaga
     3061 taagattgag atgcttgaaa tagagaataa tgctaaagat gaatctcaag gaaagagatt
     3121 agttcctttc tctagagaat tgtatataga acaagatgat tttatggaag aacctgttaa
     3181 gaaatatttt agatttttcc caggaaatga agtccgttta aaaggtgctt attttgtaaa
     3241 atgtactgat gtaattaagg atgaaaatgg aaatgtagtt gaaatacatg gtacttatga
     3301 ccctgaaact aagagtggct ctggctttac aggacgtaag gtaaaatcta caatacattg
     3361 ggttgatgct aaatcagcta taccatgtga atttagatta tttgaaccat taattcttga
     3421 tgatatacct gaaaatgaag gtaaacattt cttagaacaa ataaatccta attcattgga
     3481 aatattacaa ggttttgttg aacctacaca gataaaagat gctaaacctt ttgataaatt
     3541 ccagtttgtt agaaatggat tctttagtat agataataaa tatacaactg atgaaaaatt
     3601 tgtatttaat agaatagttc ctctaaaaag ttcttttaaa ccaggtaaat aatcttacaa
     3661 tcaaaaaatg aagacttaaa atataaatta cttctactat aaataaactt agttagaagt
     3721 aattttttat ttgagtcttc atcatttact ttaaatgatt aacttttttc actacagatt
     3781 cccatgactt ctattccatc ttttatgcat tctatgttaa gaggaaaatc tggtcctttt
     3841 tctccgtcaa tatctgttat aagtgaatct tcacattcta tatgcacctt tttagtttta
     3901 aagtatagta tttcatttcc attatattga tctaaataat cacctctaag cacacttatc
     3961 aaaactggta ttgattttgg tattggcatt cctttaaaaa taattacatc caaatatcca
     4021 tcatctattt cagccttata tgctagattt atatttcctg ctgttttgcc attaaaaatc
     4081 aacataagat acatatctcc atcataatac atttcctcag aagatacttt tatcttaaat
     4141 ttcctcatat acaatgcttc ttctattccc tttatataat aagaaattct tccaaatgaa
     4201 ttttttaagt ctgtgtttat cctttgtgat acatccgtaa acataccagc acttgcaaca
     4261 tttataaagt acttattatt gactttccct atgtctattt tctttggttt tgaatttatg
     4321 atttcattta tagcttcttt tatatcaaat gaaatttgaa gagcatttga aaaatcattt
     4381 gcagttcctg taggcaaaat tccaattgga atttttatgt ctaattcttt cattgcattt
     4441 actactaaat ctacagtacc atcaccacca gctatcaata tgtgttcata agaactatca
     4501 acttccttaa atgcattctt tacaggttct ttaatagtta atctataggg tatcaataaa
     4561 taattttttt cttgatataa ttctatgata gtgtctaaat ttgataatat ctttttttca
     4621 cctgaattag gattgtatat aagttttact tttttcatta tttctcctta ctcattttat
     4681 tcttttagta tccaaaatct tcatttaaaa aaatcacatg tattctttct ggtgttaatt
     4741 gtctttttat aagtgtataa ctacagcata ttcttcctgg acttgaacag tctatacata
     4801 taccagtagt cttacatcca gtatttgtac ttaatctttt acaattggct ggagcagcta
     4861 ttgtttttgt tctttttata gcttcatcta catctttaac tattttattt actcctgcta
     4921 taactattac tttatcaggt ccatatatca tagcagcaac tctattccca ttcccatcta
     4981 aattgtaaag ttctccatcc tctgttatcg cattagtact tgtaaaataa gcatctgcag
     5041 aaaatgactt tctatatatt tctttcattt cttctggttt gatatctttt gcatatctat
     5101 caagaaaatt atatttttct cttataaaat caatcactcc tgtttcaaat aaggtcatag
     5161 aacctccaca tcctactaga gaacctgctt taactatttc ttcaattttt ttaagaagct
     5221 cttctttgtt acttactaaa taccctttca tattattgtt ttccaaagat tttattgttt
     5281 tttctattct tttttcatta agccaattta aattttgatc cattttaact tcccccttat
     5341 ttttaatatg cacttaatta tttataaact ttattttctt tataagttta ttttagtaca
     5401 tctaaaaaaa aatacaatat aaaacttaaa tagttttaca taattttttc caaaataact
     5461 tactcaatca aaaaaggaaa gtagatgtac tattctactt tccctatatt gtatgtagta
     5521 tttattgcct aaaatctata tattaattat atgtttcata ttatataata tcacagttaa
     5581 ttaaaatgta atactatctt ctttttaaac atataaattg tattattaaa tttaatatat
     5641 ccaacttcaa taaatcttat taatttgatg aagaagatgc tatagctgca cttgttgctg
     5701 ccattacaca tgcaatttga atagcaacaa caatatttaa aaatatatta ttagttatag
     5761 agcctatatt attctcattt cgtatcaaat ctgcataaat tgaagctaca atagcagaac
     5821 taatcattac tctattactt tttcctaaag accaatttcc aaagcctttt tcttctttaa
     5881 gtttatttga aacatttttt atctcttcta ttatttcatc ttctctataa tctaataatg
     5941 aaattgctcc tattattgga tatccataat aattcatttt acaatcattt tctttaaatg
     6001 cttttttaag ttcaatagtc tttttacatt taagcatagt tctatcatca ctaaataaaa
     6061 gaatttggga taatgcttgt aaatcatttc ctttataaaa accattttca tttagaaatt
     6121 cataacattt ttctatgtat tctaaatctt catctaaatt ttttgaatga attgcaataa
     6181 gtgctatatt acaatagtca tcactagaag ttagaaatgg atgttttttc ctcatgtaat
     6241 catatattat tttcattttt gatatacatg taagataatc cattttctct ctattttcat
     6301 aaactattga tgctgtaagt ggtaaatatg tatcgcccca aaacttttca cttttaagtt
     6361 ctttgtatat atctaaaatc atcttaaaac tactttctgg atttgattca aacgaaagta
     6421 gtgttgccat ataaaacata ttatatcctc taaaattaga gaataatcca gtattttttt
     6481 taataagctc tattcgttct tccactaatc taggacttat tctcttgttc ttaagggtaa
     6541 atgcaagtgc acaactatgt tgtatcaatc ccatttgcca agaaccttta acttcattta
     6601 atgctttgta attttctatt aataagttta cttttccatt tataaacgat tgttccatat
     6661 tgccctccta gtaatatatc tcattgtact aatagtttta tatttattat atatatatta
     6721 aatactacaa tcaatacata ttacttcaaa gaacatcatt cttcaacaat aatgtagcct
     6781 tttttgtaat gtcttcccat attattgact ctatcatagt actctgattc actttcatta
     6841 ctccatttgg gcacaaatac atctattgca ttagaattac taaaatactt actagacccc
     6901 ctatccataa cagtttttaa ttttccatct acaataatct tagttccaaa acttaagtga
     6961 ttgtttgcta caaaaccatc ttctaaatca tctcctgtag cagttatcgc tccatatcca
     7021 ttttcttgat taagacttgt gtaataacta acttcaaaag ttataggtac taacttcttt
     7081 attttaatac ctaactgatt ttgtagtttc tccttttgta ctttcaactt atactcatca
     7141 ctatttttat aatcttccat accttgatta tacttggaat taaagctttt acctgacttt
     7201 actatagtat ctatatttac tatagattct ttacaatgat ctgcatctat ttttgtttct
     7261 ccatttacca ataatggagt taaacataca ctacatacaa cacatgtaat caatgcttct
     7321 ttaagcttca taaattcccc ccttattatt ttgatatatt agcatattta atattatatg
     7381 tctacattta gatttaaata ccacacccat ttgctttaag aagttgtctt atatttaatt
     7441 caccttaaac acttataaat cctctatctt agaatcatat agaataaagc ttttatcttt
     7501 aaatattttt tattaatctt ttaaaatcta aaactacttt atctaatttt ctctttttta
     7561 gaatttaaga attaaaattc tataatttta aaacatattg aatttatttt tttgagatga
     7621 tataattttc ttaagaggtg gttgatatga taaaaaatac aaaacaaatt acagtagaag
     7681 ttaatgaaat tttattacaa gaaatgtcta agataactaa tgacgaaaat aatattttga
     7741 cagatttgat aaatgaatca attgtaaatt atattcttga aaacaaaaaa cataatattg
     7801 aaaataaaga cttagaagaa gtgattaaat tttacaaaaa agaaaatact gaaaaaccta
     7861 aaagtgcagc aagagctata gctgaagcaa aaactcatac tctgtattaa tcaaataaaa
     7921 aagttcctaa aaactaggaa cttttttata ttaataatta ttaatatatc tcaatattta
     7981 tctcaaatct atcaatataa gcatgtttaa tacaatctat tgtatactta tcttacccta
     8041 gaaataatca aaacaaaaat aaaattatgc cctttatatc taaaacaatt aatttacatg
     8101 attaatatac aaaaattctg acttttaaat tcgtatacaa tatcaaacta aaataatatt
     8161 aatgacaaac ccaatataaa ttaaattcaa acaggtttga ttttacatca aacttattgt
     8221 attaatatta aataacaaga caaaaatatt tataaaaatc acaaatttta cataaaactt
     8281 tatatttgat tcatttaact aattattgta aaaactattt attttatgat aattaactac
     8341 accaaattat tcatcttttt taatatcttt aacccatttt tcaacagagt tcaattcttc
     8401 ttcatttacc ccctttttga acaaggattt cataaaactc tcaatagaac ttccatgcat
     8461 attatctaaa aaagtttttg tttcaaaatg taaatactct tcttctccaa taagaactgt
     8521 ataatgtgta tgtctatcaa tttttcttgc acttaagaat tgcttattta ctaatcttga
     8581 taaaagtgtt agtgttgtag tttgcttcca attatatagt ttttccatag cttcaatgac
     8641 atcttttgat gctactgtaa aatctgaact ccaaataaat ttcatgactt ttaattctgc
     8701 tggtggtatc ttagtaatag tcatatgatt ttacactcct ctcaaacttc tacataatta
     8761 aatactatat taatagactt ttcgagtcaa aggttttatt taaaatatct catccaaatt
     8821 tattaataca aaaaatttat atttttattc aaaattaaac acacaatcta ttaatataaa
     8881 gataaatatc tattctataa gtataacaga ttatcaaatt aaaattctaa tatttgacat
     8941 aatatataca ttttttaaat aaatatatac aatgtattaa aatgtatgca a
//
LOCUS       DAEORZ010000154         8844 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEORZ010000154 DAEORZ010000000
VERSION     DAEORZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8844
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.157"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            151..918
                     /locus_tag="KRH39_003223"
     CDS             151..918
                     /locus_tag="KRH39_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HBG8513103.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKGFCLADTSEPNYELLYFPFTDNAPIPYF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1136..1349
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1603..2637
                     /locus_tag="KRH39_003224"
     CDS             1603..2637
                     /locus_tag="KRH39_003224"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBG8513104.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVRAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKIASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2678..3895
                     /gene="argJ"
                     /locus_tag="KRH39_003225"
     CDS             2678..3895
                     /gene="argJ"
                     /locus_tag="KRH39_003225"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBG8513105.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEAHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3910..4770
                     /gene="argB"
                     /locus_tag="KRH39_003226"
     CDS             3910..4770
                     /gene="argB"
                     /locus_tag="KRH39_003226"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBG8513106.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4800..5987
                     /locus_tag="KRH39_003227"
     CDS             4800..5987
                     /locus_tag="KRH39_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBG8513107.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6096..7118
                     /gene="argF"
                     /locus_tag="KRH39_003228"
     CDS             6096..7118
                     /gene="argF"
                     /locus_tag="KRH39_003228"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBG8513108.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLV"
     gene            7245..7862
                     /locus_tag="KRH39_003229"
     CDS             7245..7862
                     /locus_tag="KRH39_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBG8513109.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(8082..8780)
                     /locus_tag="KRH39_003230"
     CDS             complement(8082..8780)
                     /locus_tag="KRH39_003230"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBG8513110.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT         3561 a          860 c         1588 g         2835 t
ORIGIN      
        1 tatatacaat ttaataattc agataatctt aaaataatat tatgtaaaat aaaaatatat
       61 atgacatgat gttgttgtat tcaaaatatt aagataaaaa tatatagaat atatttaaaa
      121 attattatgc aattagaaaa ggagataagt atgcagatta ttgatataaa tataaataat
      181 attgacaatg aacatatatg ttgttcaatt tctgaaaaaa aaggagaaaa ttgtgttttt
      241 tctaaaaaag cttggatgaa aaataatttt gataaaggtc tagtctttaa acgatttgat
      301 gtaagaggta aagcttttat agaatatatt ccagcagaaa atgcatggtg tcctattact
      361 gcggacaatt atatatttat taactgtttt tggatatctg gaaaatttaa gggtcaagga
      421 tattctaatc tattgctaca agagtgtgtt gatgatgcaa aatcaaaagg taaatatgga
      481 ttagttgtgc tttcgtctag taaaaaaatg ccttttttat ctgatgctaa atatctaaaa
      541 tataagggct tttgtttagc agatacatca gaaccaaatt atgagctatt atattttcct
      601 tttactgata atgctcctat tccatatttt aagaattgtg taaaaaaaag aaaattaaat
      661 gagaacggta tagttttata ttacagtaat cagtgtccac atacggaaaa atatgctcct
      721 attttgaaaa aaattgcaaa ttctagaggt attgatgtaa accttataaa attcgaaaca
      781 gcaaaagaag cacaaaatgc tcctgcacca tttactacat acagtctttt ttttaatggt
      841 gaatttgtaa ccaacgagat actttctgag aaaaagattg agaagttttt agataccaac
      901 attaaaaaat ttaactaaaa agagatgtaa atattgtatt ttgaaaatat attattttat
      961 aatttaatat attttggatg ttttttaaat tgacgaatga aatataaaaa ttatatcgca
     1021 atttagatac aaaatgctgg aaattaacca tatagaaaat atttccaaaa atttaaaaat
     1081 tatattgaca aagttaaaaa aataaactat catataaaac aataagaaaa taaattgaaa
     1141 tcctgtgaaa aagagagtag cataaagcta gttgatagag agctgggaat ggtggaagcc
     1201 tagtaacgaa ttttattgtg aatagagctt tggaggagat taccagaagt aatagtatgg
     1261 taattggtga atctcgcctt aagagattag agtgggtaag attatcttat caattagagt
     1321 ggtaacgcgg gtgtatattc gtctcttggt ctttgaccaa gaggcttttt taattttaaa
     1381 tgtacatttt gtagatactt tttaatttga gttttaatga ttattgtaat tttaccaata
     1441 agagtggtac cgcggaaaat cgcctcttag tatttataat gctaaggggt tttttgtttt
     1501 aattagagta ttttaatcta cgtattatca aatgattatc atatagtaaa gtggaaagat
     1561 aggattttaa gactatttga atataaaaat ggagggaaca aaatgaataa aataaaagtt
     1621 ggtattattg gtgcaactgg atatgtaggg gcagagttga ttagattatt aatgaatcat
     1681 gataaagtcg aagtgagagc gataggttca aattcgtatg ttggaaaaga tatagttgat
     1741 atatatccaa gtataggata taaaaataac atgatatgta tagaaaatga aaaagttata
     1801 gatatgtgtg atgttgtgtt tacagccctt ccacatggag ttagtgaaaa gtttgtaata
     1861 aaggcaatta aatcgaaaaa gaaagttata gatttaggtg ctgactttag aataaaagat
     1921 gaagaagtat atagtaaatg gtatggagta tcttttatag ataaaatctt acataaaaaa
     1981 gctgtatatg gattaagtga aatatacaaa gaagatataa aagatgcaga tataattgca
     2041 aatcctggat gttatccaac tagtatttct ttaccattga tgccattact aagttctaaa
     2101 ttgataaaaa ataataatat aataattgat tctaaatcag gtcttacagg tgctggtaga
     2161 gaattatcga ttagtagtca ctttacggag gttaatgaaa atatcactgc atataaaata
     2221 gggaaacaca gacatactcc agaaatagaa caaaatttaa gtgaatcctg caaagaaaaa
     2281 gtaagtgtag tttttacacc aaacttaatc ccagtaaata ggggaatctt atcaactata
     2341 tattgtacaa aagaagataa tatttctata aatgatatac ataggaaatt aactgattat
     2401 tatgaattta aagaatttat agaaattctt ccattagata agatagcttc tttaaagaat
     2461 gttagattct caaataaatg tgtaatatca ttacatgaaa atggagatac attgataatc
     2521 tgttcagcta tagataatat gattaaggga gcggcaggtc aagctataca aaacatgaat
     2581 ataatatttg gtattgaaga aaatacaggt ttaaaaaata ttgcaccatc attttagaaa
     2641 taaaaaatac taaactcata taattaggag gataaatatg gagattataa agggtggagt
     2701 tacagtttca gaaggatttt ttgcttcagg gattcactgt ggcttgagaa aaaataaaga
     2761 aaaaagagat ttagctttgg tttattcaga tgttttatgt gatgcagcag cagtagtaac
     2821 tcaaaataaa gtaaagggca atcctgttta tgttacacag gaacacatta aaaatggaaa
     2881 agcacaagct atcatagtaa atagtgctaa tgctaatacc tttaatggaa aagaaggact
     2941 tataaatgct tataaaatgg ctaaattcac cagtgataaa ttaaatatta aagaaagtga
     3001 tgttttggtt gcatctacag gtgttatagg aaagccatta aatatagatt taatagaaga
     3061 aaatatggat gaattagtga ataatctaag taaacaaggt catattggag caagagaagc
     3121 tattatgaca actgatatta taaaaaaaga aatagcagta gcaataatgt atggagataa
     3181 aaaaataact attggaggta tggcaaaagg ttctggtatg atacatccta atatggcaac
     3241 aacgttagga tttataacta cagatgcaaa tattgatgga gttttgctaa aagaagcttt
     3301 aaaaattgct gttgacaaaa gttttaatag agttagtgta gatggagata caagtactaa
     3361 tgatatggta atgattctag caaatggaaa agcaaaaaat gatagaataa acaaaaaaga
     3421 tgaacattat caagtatttt tgagtgcact tacatatgtc tgtatagaac ttgctaaact
     3481 tgttgcaaaa gatggagaag gatgtacaaa actaattgaa tgctatataa atggagcatt
     3541 aagtgaagca catgcagtaa aattagcaaa aacagttata tcttcttcat tagtaaagac
     3601 agctgtattt ggagctgacg caaattgggg tagaatactg tgtgctttag gttatgcagg
     3661 tgaagaaata gatatggaaa aagttgatat tatttttgag agtatgaaag gatatattga
     3721 agtatgtaaa aatggaaatg gattagattt taatgaggaa aaagcaaaaa aaatccttga
     3781 agatgacgaa ataagtatat tagtagattt aaatatgggc aatgccagag gaaatgcatg
     3841 gggatgtgat ttaagctatg attatgtaag aataaatgga agttatagaa attaggagga
     3901 tatactttaa tgattaatat agaaaaagca aatacactta tagaagcttt accatatata
     3961 gaaaaacatc agggaaagac tattgtagtt aagtatggtg gaagtgcaat gaaaaaagat
     4021 ggtctaaaag aatctgttat ggaagacctt gtgttaatga gttatgttgg aatcaatata
     4081 gttttagtac atggtggagg ggcagaaatc aataagatgc tagcaaaagt ggatatagaa
     4141 agtaaatttg taaatggtct tagatataca gatgaagaga ccatggaaat tgtaaaaatg
     4201 gtacttgctg gaaaggttaa taaagaccta gtaaataaaa tacatacaaa aggtggaaaa
     4261 gcagtagggc tttgtggaat agataataat atgattttat gtgacccata taaaaattat
     4321 gaacttggat ttgtaggaga aatcaaaaaa gtaaatgttg agcttataga atcttgtcta
     4381 aagtcaggtt atatttcagt aattgcaact ataggtgttg gagatgatgg agagacttat
     4441 aatataaatg gtgacacagc agcttctgct atagctaaag agttaaatgc agataaattg
     4501 atacttctta cagatgtccc tggtttgtta agagagccag atgaagaaaa atcattaata
     4561 acagaagtta tattagagga tgttgataaa ttatttgaag aaggtataat tactggaggt
     4621 atgataccta aaatagaggg atgcgtagat gccttaaata acggagtaaa tagggtacat
     4681 attttggatg gaagagttcc acattctata ataacagagc tatttactga tagtggaata
     4741 ggtacattaa taagaaaaga aaatgaataa aattataagt ttaagagggg agaataagaa
     4801 tgaataacga caatacaatt tcaaaatgga atgaatattt tatagataca tataaccaac
     4861 ccaattttgt catagattat ggtgaaggaa gttgtttttt tgatactaat ggcaataaat
     4921 acatagattt tacaagtgga tatggagtat cttctcttgg gtatagtaat agtaatttaa
     4981 agaatgctct aaaagagcaa gtagataaat tattacatac ttcaaattta tattttaatg
     5041 agccagtgtt gtattcagga gagaaaataa ttaattcaag tggaatggct aaagtatatt
     5101 tttgtaattc tggaacagaa gcaaatgaaa cagcattcaa aatagcaaga aagtatagta
     5161 gtgacaaata tggtaatggt agaggaacta tcataagttt aaaagactct tttcatggta
     5221 gaacaatgat gtctttaatg gcaactggta tggacaaata ccataagtat ttttatcctc
     5281 ttcccgaagg ttttaaatat atagaaagaa ataatattga agatttaaaa aataatcttg
     5341 attccacagt ttgtgcaata attcttgaag cgattcaagg cgaaggtgga gttaatgttc
     5401 ttgaaaaaga ttatgttttg gaaatagtaa aaatatgtca agaaaaggat attgtagtga
     5461 tttttgatga agtccaatgc ggtataggaa gaactggtaa actttttggc tatgaatatt
     5521 ttgatgtaaa accagatata gttaccgtag caaaagggtt gggagcagga ataccagtag
     5581 gaggagtatt ggtaaataaa aaactttcaa aagtactagg aaaaggagac caaggtacaa
     5641 cttttggagg taatttatta gctatggtgg cagcttcagt tgtattagat gagatatcta
     5701 aagatggttt ttataatgaa gtattagaaa aaggaaatta tataagaaaa tctattgaaa
     5761 gttttaataa caaagttgta ttaaagacaa aaggtattgg tctaatgata ggaattgaaa
     5821 ctaatattga atctagtatt atagaagaga aagctagaaa aaaaggattg cttattttga
     5881 ctgctggaaa aaatgtattg agatttttac caccattaac aatcagttat aaggaaatag
     5941 atgaggctct tgaaatccta aaagatatat tactagaaat taactaaata ttcaaaaaat
     6001 agttgattaa agataaaaat tagaagtaaa taaaaaagta aagtgctaac aagtttaatt
     6061 gaatttaaaa agtttataag aaggggcgaa ttaatatgaa taatataagt ttaaaaggta
     6121 agagtttttt gacattaaag gattttacaa aagaagagat tagatattta ttaactttat
     6181 caagttattt aaaaactaag aaaaaagttg gtataaaagg cgatttgcta caaggaaaaa
     6241 atatagcttt attatttgag aaaacatcaa caagaactag atgttcattt gaagtagcag
     6301 ctcatgatga aggagcacat gtaacttatc taggtccaaa tgattgtcat atgggtaaaa
     6361 aagagtctgt atcagataca gcaaaagttc taggaagatt ctatgatgga atagaattta
     6421 gaggctttaa acaagaaaca gttgaaagtc tagcaaaata ttctggtgta cctgtttgga
     6481 atggacttac agatgaatat catccaacac aaatactggc agatttttta acagtaattg
     6541 aaaatgtaga taaagatttg aataaagtta aatttgtata tgttggtgat gctagaaata
     6601 atatgggaaa ctcactgatg ataggatgtg ctaaaatggg aatacatttc gtagcactag
     6661 cacctaagga actttggcca aatgaagaat tggttaagga aatgaaggaa atagccaaga
     6721 aatccaaagg agatataatg ttgactgaaa atattgatga tgttaaaggt gcagatgtta
     6781 tatatacaga tgtatgggtt tctatgggag aagaggagca atatgaagca agaataaatc
     6841 aacttaaaga ttatcaagta aatatggata tgataaagaa aactgaaaat gaaaatgtta
     6901 tatttttaca ctgtcttcca gcatttcatg atttagaaac taaggttgct caggagattt
     6961 atgaaaagtt cggattatca gaattagagg ttacaaacga ggtatttaat tcaaaatatt
     7021 caaaggtatt tgaagaagct gaaaatagaa tgcatacaat aaaagctgtt atggtttcta
     7081 ctatgactga tgtaaatatg aacaataatt tagtatagat atgttttaat atagacttta
     7141 ctttaatgaa aatttataaa atattttaaa tatattattc aaaaactctc attatatgtt
     7201 aaaataactt atgtatcaac aaattaagag ggaggatttg agatatgaaa aaaatcatat
     7261 tagctttaac aactttttta ttagcaatat ctttaacagc atgttcaagt gcaaaaccag
     7321 aagatacaat tgatagtttt tttaacagtg cgaaaaagtt tgactttgaa ggcatgaata
     7381 aggtaatgga aaataatgac gaaaaatata aggatatatt aaaggaatta gatacaaaag
     7441 acccaaatgc ccaatatgtt ttagattatc taaaacagaa tgcatctaaa atcacatata
     7501 caataaaaga cagcgaggtt aaaggtgata aggctacaat caaagtagaa tgtaaattta
     7561 tagattcaac accattactt caagaaatag tagctgaggc atttacaaag atgattggta
     7621 tgagtttttc aggacaagac ttaactgatg agaaaacaac tgaaatgtta gtatcaatta
     7681 tgaaagaaaa acaaaaaagc gttaaagaga cttatgttac aaaaaatgta gagtttgaat
     7741 gttctaaaaa ggataataaa tggataataa gttcagtaaa tgatgcagta gctgatgtat
     7801 tgttatctaa tcttgttaca gcaggacaag aattttctaa ctctatgaat acacttgaat
     7861 aatattgttt aggtgtagta aagtaataaa tatatgatta tttttataaa taaacatata
     7921 aatatttatg aaggatacca tttttggtat cctttttatt aatataatga aatacatggt
     7981 taatttctat attgtcaaga aactatgata tatgttataa gtctatacta tttaagataa
     8041 acttataaca tatatttttt ataatatagt gcatgaaaaa tttatttaac taattttaga
     8101 tatttattga caactgattc agccaagcat tttgatgaat caacaatatt tattgaagga
     8161 tatgattttt ctaaaactac atttaaatca gtacaagcaa ttactatatt ttctatagat
     8221 tcatttttaa catcttcgat aagtttattc caaatatcaa tattgtgtgg attttcttta
     8281 tccattttta tattttgaat aagattgttt aattttattt gccattcatc tttaaatata
     8341 aattcatgtc cattatgtat tataccattc tggtaaattg tagactcaaa agttgaacta
     8401 gtcgaaaaaa gagttacttt ttgtgagata gtaggtaatt ttttaactgt ttcttctaca
     8461 atatttaata ttggtatatt aatagactct tttaattctt taaagtatat atgtgctgaa
     8521 ttgcatggca ttgcaataaa atttacacca gttgattcaa gcttttgtaa tccatcaatt
     8581 atagtttctt tcataagttc atgatttatt ggacggtcaa tataaaatgg ggtaggtaat
     8641 gaatatatca tcatctttgg aaattcatca tcatattttg ctccatattg agattgacac
     8701 tcatcaataa ttaaatcaac aaaaggggct gttgaccttg gacccattcc agccaaaata
     8761 ccaattaatt tttcattcat aaaaattatc tcctttatat ataaatatat ttatgtataa
     8821 aataaaaatc tataaaagca taaa
//
LOCUS       DAEORZ010000155         8815 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEORZ010000155 DAEORZ010000000
VERSION     DAEORZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.158"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..665
                     /locus_tag="KRH39_003231"
     CDS             <1..665
                     /locus_tag="KRH39_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8513111.1"
                     /translation="KSEKEKIKAEFVSEKLDMQYKYYFMVKESQEKMRQVYHDMNNHM
                     KNIKSLKNSSEDVNKYINSIEDEVKNTKNIYNTGNVLLDIILYEKSRLCMENNIDFNV
                     GIDFSKCEFIEMIDVSSIFSNLIDNAIEACSNIDDNNIEKYITIKSTFIKGYYIIRCE
                     NSKVNKVIIKNNKIFTSKKDKFLHGIGIESIKSSIRKYNGELKIKDSEYKFIVTAHIP
                     IE"
     gene            842..3409
                     /locus_tag="KRH39_003232"
     CDS             842..3409
                     /locus_tag="KRH39_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513112.1"
                     /translation="MTTFKLAVSYIKRQKGKTVALLSCIVLAVMLIFSMIVIRDSGYD
                     SQIKEAKDLHGDYHIWFEGLEKDKTQDLTNEEGISKSNTSKELCEVVDKGSGVKLYLN
                     SFDKGFLSSLGYKIEGREPIKDGEIVIEKEATRQMGISNPLNKNIDLMLLNKYIDDNE
                     INHMDSANKTFKVVGVIEKLDKYYDKSRYSITLRAFVYKDSNLPIKIKDTYNGTIYLK
                     SEKNIPQFITKMTKKIDTNVFNLHENGEVDLAKNQRQNSKFSKENIINSVLLVSVSTI
                     VIYNIFNIIFQDMTSQIGLMKSIGMSNKKVRNMLIIMSFIYIILGTLVGIVFGMIFSY
                     VGLRVVYGYSSMLTIQISSVICSFAVSIISVFISSFIVIKKSRKMSIIEAIRSSDKYE
                     KKSKNDNKKIDGKSKNLLISMATRNLWRNKPRTILTILAITLVGIMFILNLGAKSLLK
                     KNMEEGITGGSWSMSYGSVDKTVEGDSRSSESLFYKLDNNLIQKVNGMKGVRHVEPHF
                     YNHSGHILLSKNKLSKAYKDELDRKNSSYQEEHNNEYPLLIRGYSDDMLKQRQEFIEK
                     GENILSTTSGKYKKVILVNNTNSQVTHSFDAKIIDDVKIGDIIEIKLPVYRDGIEKYE
                     NFKVEVGAIMKELYASSQDGNTQAQGAQMIFKENDYKELTGQKEYNKLFVIVEKGQLY
                     SVERRLEETTKNYGSTEINGKGEELKLIGMQQSSEERLSVIYQFLTILILAVNIIFIM
                     RSNIITRRKELATLRAIGMSIKSIKKILIIESQMYGMVASIIGAIIATVYHNYGLAKT
                     NKVLLEGGYTRIIEPNIPFSQIIILFTIFIAMGFISIYVSKDKIEGTTITEGISQNN"
     gene            3468..4151
                     /locus_tag="KRH39_003233"
     CDS             3468..4151
                     /locus_tag="KRH39_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513113.1"
                     /translation="MENRIMKKLAIKNMKKVYGKGDNKVYALNGIDLEIQPNKFTTIV
                     GQSGSGKSTLLHCMAGLDKPTDGKVFIDDLDLYTLNDDKLSKIRREEFGFIFQSYNLI
                     PVINVYDNIVLPISISKRKIDKDYIIDLIAKLGIKSQIDKFPNELSGGQQQRVAIARA
                     LANKPSIIFADEPTGNLDSKTTNEVVRLLKLCVYEYKQTLVMITHNAEIANNSDNIIN
                     ISDGKIVSI"
     gene            4414..5274
                     /locus_tag="KRH39_003234"
     CDS             4414..5274
                     /locus_tag="KRH39_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG8513114.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKVLNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEVGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            5264..7135
                     /locus_tag="KRH39_003235"
     CDS             5264..7135
                     /locus_tag="KRH39_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8513115.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRLTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSVKGEGKKEIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWSMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIIGVVVSFAAATVASYLLGIP
                     SDEKEEVKDDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            7155..8609
                     /locus_tag="KRH39_003236"
     CDS             7155..8609
                     /locus_tag="KRH39_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG8513116.1"
                     /translation="MNIKDIFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFNALRISIDWSRIFPNGDDNNPN
                     ELGLKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKIGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         3538 a          906 c         1585 g         2786 t
ORIGIN      
        1 taaaatcaga aaaagaaaaa attaaagcag aatttgtaag tgaaaagtta gatatgcagt
       61 ataaatacta ttttatggta aaagaatcac aagaaaaaat gagacaagta taccatgata
      121 tgaacaatca tatgaaaaat ataaaatcgc taaaaaacag tagtgaggac gtaaacaaat
      181 atattaatag tatagaagac gaagtaaaaa atactaaaaa tatttataat acaggaaatg
      241 tactattaga tattatcttg tatgaaaaaa gtagattatg catggagaat aatatagatt
      301 ttaatgtagg catagatttt agtaaatgtg aatttataga aatgattgat gtaagtagta
      361 tattttcaaa tttaatagat aatgcaatag aagcatgtag taatattgat gacaataata
      421 tagaaaaata tataactata aaaagtacct ttataaaagg gtattatata atcagatgtg
      481 aaaacagtaa agtgaataaa gtaataatta aaaataataa gatttttact tctaaaaaag
      541 ataaattcct tcatggcata ggtattgaga gtataaaatc ttcaataaga aaatacaacg
      601 gagaattaaa aattaaggat agtgagtata aatttatagt tactgcccat ataccaattg
      661 aataaaattt caatagattg atgcatttaa aaagcttata agctagtctt taatatgcta
      721 gttggcggtg aaaaaatacc acttgtcgga cttatattga ataaaaattt taaaaatgtt
      781 acctttacaa aaaaggtagc attttttata caaggattta atagaagaag gaggtaaagt
      841 tttgactaca tttaaactag cagtttctta tataaaaaga caaaaaggaa aaacagtagc
      901 actactatcg tgtatagttc ttgctgtaat gcttattttt tctatgattg tgattaggga
      961 ttctggatat gattcccaaa taaaagaggc aaaggattta catggtgatt atcatatatg
     1021 gtttgaggga cttgaaaagg ataaaacaca agacctcaca aatgaagaag gtatatcaaa
     1081 gtcaaacact tcaaaagagc tatgtgaagt tgtagataaa ggaagtggtg ttaagcttta
     1141 tttaaattca tttgacaagg gttttctaag ttctttggga tataaaatag agggaagaga
     1201 acctataaaa gatggagaaa tagtaataga aaaagaagct acaaggcaaa tggggatatc
     1261 taacccatta aacaaaaata tagatttgat gcttttaaat aagtatattg atgacaatga
     1321 gattaaccat atggatagtg caaataaaac attcaaagtt gttggtgtga tagaaaaatt
     1381 agataaatat tacgataaat ctaggtatag tattacactt cgagcttttg tgtataaaga
     1441 ctccaatttg ccaataaaaa taaaagatac gtataatgga acaatttatc ttaaatcaga
     1501 gaaaaatata cctcaattta taactaagat gacaaaaaag atagatacca atgtctttaa
     1561 tctgcatgaa aatggtgagg ttgacttagc aaagaatcaa agacaaaatt caaaattcag
     1621 caaagaaaat attataaatt cagtgctttt agtcagtgta tcaactattg taatatacaa
     1681 tatttttaat attatttttc aggatatgac tagtcaaata ggtcttatga aatctattgg
     1741 tatgtcaaat aagaaagtaa gaaatatgct tattattatg agctttatct atataatttt
     1801 aggaactcta gtaggaatag tatttggaat gattttttca tatgttggtt taagagtagt
     1861 gtatggatac agctctatgc taacaataca aatatctagt gtaatatgtt cttttgcagt
     1921 ttcaatcata tcagtattta tttctagctt tatagtaatt aaaaaatcca gaaaaatgtc
     1981 tataattgag gctataaggt caagtgataa gtatgaaaaa aaatcaaaaa atgacaataa
     2041 aaagatagat ggaaaaagta aaaatttact tataagtatg gcaactagaa atctatggag
     2101 aaataaacct agaacaatat taacaatatt agcaattaca ttggttggaa taatgtttat
     2161 cttaaatcta ggtgctaaaa gtttgctaaa aaagaatatg gaagagggga taacaggtgg
     2221 ttcatggtca atgtcatatg gaagtgttga caaaactgtt gagggagatt ctagaagttc
     2281 agaatcttta ttttataaat tagacaataa tttaatacaa aaagtgaacg gcatgaaagg
     2341 tgtaaggcat gtagaaccac atttttataa tcatagtggc catattttac tttctaaaaa
     2401 taagttatca aaagcttata aagatgaatt agacagaaaa aattcctctt atcaagagga
     2461 acataacaat gaatatccac ttttgataag agggtatagt gatgatatgt taaagcaaag
     2521 acaagaattt attgaaaaag gggaaaatat actaagtact acatctggca agtacaagaa
     2581 agtcatctta gttaacaata cgaattcaca agttacacat tcttttgatg caaagataat
     2641 tgatgatgtg aaaataggag atattataga gataaagcta cctgtatata gagatggtat
     2701 tgaaaaatat gaaaacttta aggtagaggt tggtgctatc atgaaagaat tatatgcatc
     2761 aagtcaggat gggaatacac aagctcaagg ggctcaaatg atatttaaag aaaatgacta
     2821 taaagaatta acaggtcaaa aagagtataa taagcttttt gtcatagttg aaaagggaca
     2881 attatattct gtagaaagaa gattagaaga aaccactaag aattatggat ctactgaaat
     2941 caatggaaaa ggagaagaac taaaacttat tggaatgcaa caaagttcag aagaaagact
     3001 ttctgtaata tatcagtttt tgactatatt aatacttgcg gtgaatatta tttttattat
     3061 gagaagtaat attattacaa gaagaaaaga gctggcaacc ttaagggcaa ttggaatgag
     3121 tataaaaagt attaaaaaga tactaattat tgaaagtcaa atgtatggaa tggtagcttc
     3181 tataatagga gctataattg caacagtata tcataattat ggtttggcta aaacgaacaa
     3241 agttcttttg gaaggtggat atactaggat aatagaaccc aatattcctt tttctcaaat
     3301 aattatacta tttacaattt ttattgctat gggatttatt tctatatatg tatcaaaaga
     3361 taagatagaa ggtacaacaa taacagaagg aatttcacaa aataattaag gctatggaat
     3421 gacaagataa aaatgtaata ctatttagtg ggatttaaat aagaagaatg gagaaccgta
     3481 ttatgaaaaa attagctata aagaatatga agaaagtata tggaaaaggg gataataaag
     3541 tatatgcatt aaatgggata gatttagaaa tacaacctaa taaatttaca actatagttg
     3601 ggcaaagtgg ctcaggcaaa agtacattac ttcactgtat ggcaggtctt gataaaccta
     3661 cagatggaaa agtatttata gatgatttag atttatatac cctaaatgat gataagttat
     3721 caaagataag gagagaagag tttggattta tatttcaaag ttacaatcta attccagtta
     3781 taaatgttta tgataatata gtacttccca tttcaattag taaaagaaaa atagataaag
     3841 actatataat agatttaata gctaaactag gtataaaatc tcaaattgat aaatttccta
     3901 atgaattatc tggaggtcaa caacaaaggg tagcaattgc tagagcactt gcaaataaac
     3961 cttcaataat atttgcggat gagcctacag gaaacttaga tagtaagaca actaatgaag
     4021 ttgtgagact tctaaaactc tgtgtatatg agtataaaca gacattagtt atgataactc
     4081 acaatgctga aatagctaat aattcagata atattataaa tattagtgat ggtaaaatag
     4141 tatcaattta gtatataaac tttgcaagat aatcgcctac agagtctgta gttatataat
     4201 taaaaaaata tttaggaaac cgttgacaga tttacataga tagtataaca tataggatat
     4261 aaaattaaac gaaaaagaat tctaattatg gcgacgtagt cataattttg agtatgttac
     4321 tgattagcag gcagaactca agtatatatg tgaaaaatag agggatactc tatacatata
     4381 tgcttgagtt ctttttattg aaaggaggaa tatatgaaaa taattcagat tttaaataat
     4441 aatgtagcct tagtaaaaag aggaaaaaat gaaattattg tatttgttaa aggaattggc
     4501 tttaaaaaaa gagtgggaca ggtaataaat gaaaatgaaa tagagagaac atatatacta
     4561 gattcatacg atatgctaga acactttagt tatctactac aaaactcaga ttctaacaat
     4621 atagttctta ttaatgaaat aattgaatat ggtgagaaag ttttaaatat aaaatcaagt
     4681 gattatctaa gtttaaccct actagaccat ttagagtttt tacttaaaag atgtgaaaag
     4741 aatcaattta taaaaagtcc tttggtatgg aatgttaaaa gattttatcc aaaacatttt
     4801 gaagttggta tgcatgcact aaaattaatt ggtgatagta agggaattaa tcttcctgat
     4861 gatgaagcag tatcaatagc acttcatttt gtaaatatgg aggtaaataa agagtctcat
     4921 gatagtacga ttgtagagtt aagaacttta gcagatatag tttctataat aaaatatcat
     4981 tttaatgtag agctagatga aacttcaaca aactatatga gatttactac tcatctacag
     5041 tactttatcc aaagaattat gtcagatgaa gcttcaatag atgaagataa ttctatagat
     5101 ttatataaac aagtaagtca actttatcca aagtcatttg gaagtgttca aaagataaga
     5161 aaatatataa aatcacaatt taatgtcgat atatctataa gtgaagaaac ttatttaatg
     5221 ttgcatataa atagagtgac tcaaagactg gaggcaaaat cagatgaaat ataaagattt
     5281 aaatgaaaat ataataaaac ttataggggg aaaagaaaat ataaagtcgg tagcacattg
     5341 tgttactcgt ttaagactta ccttaaatga taggagtatc gctcaaacag aagaaattaa
     5401 aaaattaaaa ggagtaattg atgtagtttc taatgatgtt gcttatcaaa ttataatagg
     5461 tactcatgtt gtagatgtat ataacgagtt tatgtctatg ataggtttaa gttcaagtga
     5521 atcaagtgta aaaggtgaag gaaaaaagga aattagaggt atcaaaggta tatttaattc
     5581 catctttgtg gtagtctcag agactatgac ttcagtaatt gaagtactac ttgcagctgg
     5641 tatacttgca agtatattag cacttttaaa tatgtctggc atacttcaaa gtgatagtcc
     5701 tacatatcaa atattagata cattaagaag cgctacattt cacttcttac cagtattaat
     5761 tgcaatagca tcagcaaaaa gacttaatgt aaatccctat atagcagttg tgttagcagt
     5821 tacgttatta tcttcatcaa tagatggtgt agaaggatta agtttatttg gtataaattt
     5881 acaggctaca acttatgcaa acagctttat tccaatactt ttaggagtat ggtcaatggg
     5941 aattataatt gaaggtttga aaaaaatatt acctaaagca ctacactatt ttttagttcc
     6001 agtattatct ttaataataa ctttatcagt tacattaact gtatttggac ctattggtat
     6061 gtggattggt gatggattaa actatgcatg tatgttttta attgatacat tgggtaattg
     6121 gagtgttgtt gcactgtatg cagctttaca accactctta atagtttttg gtgcagcaaa
     6181 ctttactatg cctatatctt taaattctgt gacaacattg gggtatgacc caatttttat
     6241 gggcgctgca acaatatctg atattgcagt ttgtggtgca atgtttggat atttcttaaa
     6301 aactaggaat aaagaacaaa agcaattatt tggtacagta agctttagtg cacttatggg
     6361 agttacagag ccttcagtgt ttggtgtgtt tatgaaatat agaagaccat ttgtagcagt
     6421 aatgataggt ggaggtatag gtggtttaat agcaggttta gcacaagtta aaacatatgg
     6481 aatggtttgg ggattagctg cattaccaac atatcttgtt ggaggagagg tatctaactt
     6541 tacgtttatg ataataggtg tagtagtatc atttgcagca gcaacagtgg cttcctattt
     6601 attaggaata ccttcagatg aaaaagaaga agttaaggat gataatgaaa aagaattaga
     6661 actaataatc aacgagaata attcaagaac tattagtatt ggaaatgttg caaaaggtca
     6721 ggtaattgca cttggggaag ttaatgataa agcattttcc tctggagcat taggtaaagg
     6781 ggttggagtt atacctagtg aatcaaaatc aactgttata tctcctgtag atggaactat
     6841 aactatagta tttccaacta aacatgcata tggtatagtg actgatgaag gattagaaat
     6901 attaattcat attgggatag atactgtaaa cctagatgga aagttctttg aaagtcttgt
     6961 aacacaaaat caaagggtta aaaaaggaga tgctttagca gtatttgaat cagataaaat
     7021 tatagaggaa ggttttgatt caactataat aactgtagtt acaaatacta gcgattattt
     7081 agatgtaatc tcatcaaatg atgaatcaga agaattatta actgtaatag tataaatttt
     7141 aggaggggaa attcatgaat attaaagata tatttttctg gggaggaagt attgcagccc
     7201 atcaatgtga aggttcatgg gatagtgata acaaaggacc tgcaattatg gattttgtaa
     7261 ctaagggctc ttatgaaacg ccaagagtaa ttactgataa aatagaagaa aaattggatt
     7321 atccatctca taatggaatt gatttttata atagatataa agaagatata gctttattta
     7381 aagaaatggg atttaatgca ttaagaatat ctatagattg gtcgagaatt tttccaaatg
     7441 gggatgataa caatcctaat gaactagggc ttaaatatta tgaaggtctg atagatacat
     7501 taatagaaaa taatatagaa cctattgtaa cactatatca ttttgaatta cctatgaatt
     7561 tagtacataa atatggttca tggaataaca gaaaacttat agatttgtat ttaaagtaca
     7621 gtgaaactgt aataagaagg tttgatgata aggtaaagta ctgggttaca tttaatgaga
     7681 tgaaccatat tgaccctcaa acagagcatt cggatatttt tacttatatg atagcaggta
     7741 ttaagtatag tgaaatagaa aataaggttc aaacattggc aaatgtaggg tataatatga
     7801 ctttggcggg tgtaaaggtt gcaaagttgg ctagagaaat caatcccaac aataaaattg
     7861 gatgtgtatt tggactaaat ccaatttatc catacaattg taatcctagt aatgtgttaa
     7921 atgcattttt agataatgac cgtgattatt atcaaattga tgcaatgtgc aatgggaagt
     7981 ttccacaata taaattaaaa gaatatgaaa aaaataatat aactattgaa gtgactgatg
     8041 aagacaaaaa agcatttaaa gaaggtaagt tagattttat aggacttaat tattattttt
     8101 caagtgtatc tactccaaaa gaaaatttat caggtgataa atctttattt ggtgggattc
     8161 aaaatccata tttggaacaa agtaagtggg ggtgggcaat agaccctata ggtttaagat
     8221 ttaccttgaa ctatatttac agaaagtatc aattacctat cttaattaca gaaaatggtt
     8281 taggggcata tgataaaatc gaagaagatg gaactataaa tgatgattat agaattgaat
     8341 atttaagtaa gcatctatct caaatgaagt ctgcaattga agaagataat gttgaatgtt
     8401 ttggatattt gatgtgggga ccaattgatt tagtcagtgc aactacaggt cagatgagta
     8461 agagatatgg atttatctat gttgacttag atgatgaagg caaaggaagt tttaagagat
     8521 ataagaaaaa atcatttgat tggtacaaag aagtaataaa atctaatgga gaagttttag
     8581 atttttctac ggatatagta gtagggtagg aataagataa atagaagtta ggaactaatg
     8641 ttaattagtg tctagtaaag catttaaaat agagtatagg gtctttaaag taaagtgtgt
     8701 attcttctta aaaataatgg caattaaaat tactataaaa aggagaatac acatttttta
     8761 tttttatact taagtaaata tttagacata ttcatagtat aatttactca ttata
//
LOCUS       DAEORZ010000156         8811 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEORZ010000156 DAEORZ010000000
VERSION     DAEORZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.159"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            69..1262
                     /locus_tag="KRH39_003237"
     CDS             69..1262
                     /locus_tag="KRH39_003237"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBG8513117.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIIWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1468..2586
                     /locus_tag="KRH39_003238"
     CDS             1468..2586
                     /locus_tag="KRH39_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBG8513118.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKKAID"
     gene            2717..2962
                     /locus_tag="KRH39_003239"
     CDS             2717..2962
                     /locus_tag="KRH39_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBG8513119.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            2949..3695
                     /locus_tag="KRH39_003240"
     CDS             2949..3695
                     /locus_tag="KRH39_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG8513120.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            3978..4853
                     /gene="hslO"
                     /locus_tag="KRH39_003241"
     CDS             3978..4853
                     /gene="hslO"
                     /locus_tag="KRH39_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBG8513121.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5244..6044
                     /locus_tag="KRH39_003242"
     CDS             5244..6044
                     /locus_tag="KRH39_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG8513122.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEYKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKRERKIKDDLYFIKPKYVKYIPRNERAIYKALDFND
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            6572..>8811
                     /locus_tag="KRH39_003243"
     CDS             6572..>8811
                     /locus_tag="KRH39_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012580989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG8513123.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
BASE COUNT         3469 a          874 c         1737 g         2731 t
ORIGIN      
        1 aggttataat aaatatgtgc gtaaaatcta acaataagca ataataccgt aaatttagga
       61 ggaataatat ggatacttta aaggaacttt ttaaaatagg aagtggacct tcaagttctc
      121 atactatggg accacaaaga gcagcagaga gatttaagaa tgaaaaccca gatgcagaaa
      181 gttttagagc aatattatat ggtagtttgg cagctacagg gaaaggtcac ttaactgact
      241 atataattga aaaaactata gcacctaaaa aagtcgaaat tatttgggaa gaagatatta
      301 taaaagattt tcacccaaat gggatgaagt ttgaagcttt agataaagat aaaaatgtaa
      361 cagctgaatg gactgtatat tctgtaggtg gaggaactat agcagaagaa ggtcaaagaa
      421 acagtaaaag caatagtata tatcacttag atactatgga tgagatagta aaatggtgca
      481 aagaaaataa taagactttg gtagattttg tgttggaatg tgaaccaaaa gacataaaag
      541 attatataaa aactataaaa gatgctatga gaaaatctat tgatgatgga ctaagtacag
      601 atgaaataat accaggcaag ttattattaa aaagaagagc aagtacattt tataatgcat
      661 ataaaaaaga taagagcttc tcaacacttg tatatgcata tgcactagca gcttcagagc
      721 aaaacgcttc tggtaatata atagttacag caccaacttg tggttctgct ggagtaatac
      781 caggaatatt ctttgcaatg caggatttct ataattatga tgacgaaaaa ataattgaag
      841 cactacttgt agctggaata ataggtaata taataaaaac taacgcttca atatctggag
      901 cagaggtagg gtgtcaaggt gaagttggag ccgcttgttc tatggcagca gcagcagtag
      961 catacttaaa aggtggaact atagaccata ttgaatatgc ggcagaaata gcacttgaac
     1021 atcatttagg aatgacttgt gaccctgtat atggatatgt tcaaattcca tgtatagaaa
     1081 gaaatgcaat ggcagctcaa agagcttatg atgcagctaa ttatgcatta ttaactgatg
     1141 gctcacattc tgtctcctta gaccaagttg ttgaaacaat gaaagaaact ggtatagata
     1201 tgatggataa gtacaaagaa acagctaagg gcggacttgc taaacatttc ttctcttgct
     1261 aaaaataaaa ttttccaata ataattcctc tgttttgatt tatgaaacag gggaattatt
     1321 attaatggtg aattatttaa taatgcaaaa tatttttata ttcaatttag ctttttattt
     1381 ttaattagta taacatgcta tataatattt taattataaa gaaatataga aatatttttt
     1441 aataaattag aaattggagg agattacatg agagaggcat catatttaca agaacaactt
     1501 tataaacata ggaagtcatt aaatcctatt gcagagattg ggagaaaaga gtataagact
     1561 tcaaaatata taagagatta tcttgataaa ttaggaattg agtatgaagt ttatttagaa
     1621 acaggtatag taggtgtaat agaaggtaaa agacctaaaa aagatattgc ttttagagct
     1681 gacatagatg gtcttcatag tgaagaaggg gtaaaacatt tatgtggtca tgatggacat
     1741 gctactatat tacttggatt aattgagtat ttaaatgata ataaagaaga cttaaatgat
     1801 aatatagtat ttatattcca accagcagaa gaaggtcctg ggggagcaaa agctttaata
     1861 gatgaaggag tgcttagaaa atacaatatt gatgaaatat atgggcttca tatatatcca
     1921 gagttaccag aaggttatgt aggtacaaga gagggctatt ttatggctca aataggagat
     1981 attgacatag aaatagtgtc taaaagtgga catggggcta tgccacaaaa tggtattgat
     2041 ggtatagtga tagcttcaaa ctttgtaagc tcacttcaaa ctatagtgtc tagaaatata
     2101 agcccaatag acaatgcagt gttgactatt ggtagaattg aagggggagc tagaagaaat
     2161 ataatagctg aaaatataaa gatggaaggt actatgagat gtttcagccc agaagtgtat
     2221 gagaaaatga agcttagagt tagagaactt gctagagggt ttgaattggc ttataactgt
     2281 aaggttaata taaatattat agatgattat attgctgtta aaaatgacaa ggatttgtat
     2341 caagagtttg tagaggctat tggaagtgat actgttgtag agttagagcc acttatgata
     2401 tcagaagatt tttcttatta tcaaaaagaa gttccaggat tattttttat gttgggttca
     2461 agaaacgaag aaaaaggatt tgtaaatggt cttcataata taaactttaa ttttgatgaa
     2521 aagatatgtg taaatgcatt gaatgtttat tcaaaattat taaaatacaa aaaagcaata
     2581 gattaggaag ataagttata aagattaaaa agcataaata cctaaattta aaaaaagtgt
     2641 attaataaat accccttaca tatttgtata aatttatttt aattgacaaa aatacaaata
     2701 agaaggggtg atttttttga aagaaaaagt acaaagagaa aagaagataa ctaatggcaa
     2761 gaatactaaa atattgacaa atgatgatat aatgaaatat gagatagcct cagaactagg
     2821 tcttatggat aaagttggtg aacgtggatg ggcaggactt acagctaagg aagcaggaag
     2881 aattggaggt atgcttacat cacgcaaaaa gaggcttaaa aagattgtgg aggagcagaa
     2941 taaaaaggat gagtcaatat agtgattttg cgtttgtata tgatgaactt atgaatgaag
     3001 ttgattataa tggttgggtt aaatatatag aagatataat agatagtgaa ggtgtaaaag
     3061 taaaaaatat attggaattg gcatgtggta cgggtaatct tacaattcca ttgactaaaa
     3121 aaaattacga tatagctggt atagatatat ctgatgagat gcttagtgta gcaagagaaa
     3181 aggcagaaaa agaaggagta gaattagttt tacttcaaca agatatatca gagcttgatt
     3241 ttgaaatatc tgatttggac tgtgtgcttt gtgcttgtga tggatttaat tatataactt
     3301 atgatgatga tttggaaaat gtattttcaa agacatatga attattaaag aaagatggaa
     3361 tattcatatt tgatataagt tctttttata aattagccaa catattggga aataacatgt
     3421 atggagaaaa tagagaagat atagcgtata tgtggcaaaa ttactttgat gaagaagaga
     3481 atctaattga aatggaactt acattcttta ttaaagatga atgtggaaag tttgaaagat
     3541 ttgaagagat acaccaacaa agagcatata ctgaagatga gatagttgat atgcttgaaa
     3601 aatctggatt taaggatata aaatattatg gggattttac atttgacaaa cctagtgaag
     3661 aaagtcaaag aatcttcttt gtatgtaaaa aataagatat tcttgactat ataagataat
     3721 tattaaaaat aaccatgata taaaaatata gaaaatgcag ttttatagat taaaagtaaa
     3781 gtgatgatac tttatgactt ttattttcta ataattataa aattctatag ataaaaatta
     3841 aaatatagta actattactt agtaaaatta aaataaaatg ggttttgccc aaggttaaga
     3901 tagataagta tttatagata aattaaggta gtaatataat aaaaataaga caattaacgg
     3961 gacatggagg aatattaatg agagattatg tactaagagc gacatcagga aatggtcaag
     4021 ttagagcatt tgtcgcaact actagaaata cagtagaaga agctagaaga ttacatgaaa
     4081 ctacaaaagt agcaacagca gcacttggaa gaacattaac agccacatct ataatgggat
     4141 taatgatgaa aaatgatagt gataagttga ctgtaataat aaaaggtgga ggtccaattg
     4201 gaactattat cgccacatca gattctaagg gaatggtaaa aggatatgta ggaaatccac
     4261 aagtagaggt tgaagactat ccaaatggaa agttaaatgt agcagcagca gtaggtacag
     4321 aaggtgttgt aaaagttata aaagatttag gtcttagaga accatataat gggacttatc
     4381 cattggtaag tggagaaatt gcagaagact ttacttatta ttttgctgta tcagagcaga
     4441 ctccatcagt agtagcttta ggtgttctta caaaagaaga tgaagtagag tttgctggag
     4501 gatttatagt gcaattaatg ccagatgcag aagaagagac tattgctaag ttagaggaaa
     4561 atgtagctaa attaccttct ataactaaca tgttaaaaga aggaaaatca ccagaggata
     4621 tattaaatat tgttttggat ggtttggaac ctaaaatact tgatacctgt gaagttgggt
     4681 ttatgtgtga atgttcaaaa gaaagagtaa aaactgcttt ggttgcaatt ggtaaaaaat
     4741 cattggctca aataatagaa gaagataaaa aagcagaagt tggttgccag ttctgtaata
     4801 agaagtatat gtatagtgaa gaagagttgc ttgaaatttt aaaggaaatg tagactataa
     4861 aaatagttgt attgatataa gtaatgttat agtgatatag taaagtcgta ttgatatagt
     4921 aattaaagta taatttatta ataatagaca tatttatatt aaaaaataag tttaataaat
     4981 ttttaagctt aataaatttt aagaattaag aagctttctg gctgtaatat ttgcaatgtt
     5041 gcagttagaa ggctttttta ttttattata gttcgtgtca aagattgtat gaaacaataa
     5101 aatattgtaa aataatttgt atatttagac ctgattgaca caaaggttta taagatattt
     5161 gagttgatta tacgaaattc tatgaagtag taaccctgtg actttaatca tgggaggttc
     5221 agaagaagga gaaaatttta aatatggaaa gtaaaacgaa gtacatagga ataagtatat
     5281 taatattatt gatgatattt ttaattgcaa agaattttaa tggaaaactt gaaataccta
     5341 ttcctgagag taatagtaga aaatttaaat cagcagcagt gttttatcct caacatcaag
     5401 atgatgaggt tttatgggga ggaagtgcca ttgtagatgc tataaaacag tgtggtgttg
     5461 ataatgtata tgtggtatta gtatcagatg gttctggagt taacgttttc aaagctaaca
     5521 caaaatttag aaacttaact aggaagcaaa aggaagaact taggaataac gagtttaaat
     5581 cagctttgag ggagcttgga gtgaaacctc aaaatgtgat tatacttgcg gatatagata
     5641 aaaaggaagg cacacattat gaattaatgg aaaagactat actggatttt gaatataagt
     5701 ttaaaggcaa tgtgacccat atagcccatc actataagta tgatgaccat ataatgcata
     5761 gaaagaatgg ggaagtttta aagaaactta aacgtgaacg taagataaaa gatgacttgt
     5821 attttattaa gccaaaatat gtaaagtata taccaagaaa tgaaagagct atctataagg
     5881 cactggattt taatgactat aataaggtta agagtgcttg ttatgaatat aaggttgtaa
     5941 attcaaagcg taaaatgtat ggtataggat atatttcggc acatagttat tttgaacatc
     6001 ttctaaatga ccctgaactt acgtctgtat taagtgaaga ttaatttctt ataagtagta
     6061 agttatgttt gataagttga ttggaatttt ctatagtttt gaaggtattg gaaatttagt
     6121 tttatttaaa gatttaaaga tagaggtttt tagtgtttaa aattttaaag ttaagttgaa
     6181 taattgtata gaattatttg tgaaagaaga aacagtgtaa aacatagtat cttatttatg
     6241 gttttaaaat cataggcaag atactttttt tatgtaacta ataccatttt gagattatta
     6301 acttaagaat tttatataat ttaacactca tatataatga tgatatttag gtagaataga
     6361 tgtaattacg cttaaataaa ataactgtat tttttataaa aatccaaacc ctacactata
     6421 ctaccagata aaatctacat gctataattt gacctgtgtt tataaaaaaa ggagtaatct
     6481 aataaaatag tatttcactt atgtataact aacaactatt attacgattt tctctaaata
     6541 atttaaagaa aaataatgca ggaggtaaaa tatgaacaat gaatttataa ttttaaaagg
     6601 ttgcaaagag aacaatttga acaatatttc cttaaaaata ccaaaaagaa aaataaccat
     6661 attcacaggg gtgtcgggtt ctggaaagtc atctattgta tttgagacaa ttgcaaaaga
     6721 atcacaacgc caactaaatg aaaggtttag tacatttgta agaagttttt tacctaagta
     6781 tggagaggtt aaagctgatt gtatagaaaa tttgtcaaca ccaattatca tagaccaaag
     6841 tagacttggt ggaaattcaa ggtcaaccct aggtactatt acagatgtaa attccttttt
     6901 aagagcttta tattcaaggt ttggtagtga gtatataggt aatgctaata tgttttcttt
     6961 taataatatt gatggtatgt gcccacactg tcatgggctt ggaaaaaagc tagttcctaa
     7021 tatgaaccaa attcttgata tgaataaatc attaaatgaa ggtgctatat tgctttcagg
     7081 gtttggagtg ggttcttggc attggaatat attcgctgaa tcaggatttt ttgatattaa
     7141 taagaggata tgtgattatt cagaagtaga attggaaaag tttttgcatg gtaaagcaga
     7201 aaagattaag atagaaaatg ctggtcaaac taacatgaca tatgagggat taatggttaa
     7261 atttaatcgc ctatacttaa gtagagaggg agaaatatct gaagctacta agaaaaaatt
     7321 gagtaaactt cttatagaag atagatgtcc tatatgtgat ggaagaagac ttaatgaaag
     7381 agtatatagg tctttaatta atggttataa tatagcagat ttaactagta tgcaaattga
     7441 tgaattggct gaagttatta aaagtataga tgagccagaa gcacaacctt taattaaagg
     7501 aataatagaa aagttaaaca gtatcataga tataggtctt ggatatttaa cactagatag
     7561 agagacatct tctctatcag gaggggaatc tcagcgtata aagatggtta agtatttaaa
     7621 tagtaatctt gttgatttaa tgtatatttt tgatgaacca agtgtaggac ttcatccaaa
     7681 agatgtgtat aaattaaata atcttttaaa aaaattgcgt gataaaggga atacaattat
     7741 agttgttgaa catgacccag atgttattaa gattgcagac catattgtag atgtaggacc
     7801 aaaggcaggt aagtatggtg gagaagttgt gtatgaaggc agttatgaaa atctacttac
     7861 aagtggtact ttgacaggga atgctttaag taaatttctt cctattaaag aaagtgtaag
     7921 agaacatagt ggatatttag aagttaaaaa ttgtaataaa aacaatttaa aaaatgtatc
     7981 tattaagata cctaaaggtg tattgactct agttactgga gtagcaggag caggtaaaag
     8041 tacgttgatt aaggatgaat ttttaaagca aaatcctagt gctgtactta tagaccaaag
     8101 ccctgtttca gcaaattcaa ggtcgagttt ggcaacatat agtggaatta tgaacaacat
     8161 aagaagcata ttttcaaaaa caaatggcgt aaatgcttct ttattcagtt caaattcaga
     8221 aggagcttgt gataactgta agggttctgg aatagtagaa atgaatcttg cttttatgga
     8281 gagtataaag agtacctgca atgtttgtga aggtaaaaaa tttaaaaaag aagttttaga
     8341 atataggttt caagggaaga atataattga agttcttgaa atgtctgttt tagaggcaat
     8401 tgagtttttt aatttaaaac agattaaaac gaaattacaa tgtattgaaa aaatgggtat
     8461 tggatacctt actttaggac aaactcttga tactttgtct ggaggagagt gtcaaagatt
     8521 aaagcttgct agtgaattaa ataaggaaag ttcagtgtat atattagatg aaccaacaac
     8581 aggtcttcat atggctgata ttgagaattt tataagtata attgaagaga ttgtagataa
     8641 tggaaatacg gtcataataa ttgaacacaa tatagatgta attaaaagag ctgattggat
     8701 tattgagctg ggacctgaag gaggaacaaa aggcgggaga gtaatatttg aaggtattcc
     8761 aaaacaatta tgtaacaata aaatatcatt aacagcaaaa tatatagtat a
//
LOCUS       DAEORZ010000157         8685 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEORZ010000157 DAEORZ010000000
VERSION     DAEORZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.160"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            86..967
                     /locus_tag="KRH39_003244"
     CDS             86..967
                     /locus_tag="KRH39_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8513124.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            complement(1155..2147)
                     /locus_tag="KRH39_003245"
     CDS             complement(1155..2147)
                     /locus_tag="KRH39_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBG8513125.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEGMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(2152..2892)
                     /locus_tag="KRH39_003246"
     CDS             complement(2152..2892)
                     /locus_tag="KRH39_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBG8513126.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            complement(2957..4066)
                     /locus_tag="KRH39_003247"
     CDS             complement(2957..4066)
                     /locus_tag="KRH39_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBG8513127.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDVVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            complement(4084..5217)
                     /locus_tag="KRH39_003248"
     CDS             complement(4084..5217)
                     /locus_tag="KRH39_003248"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBG8513128.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIINKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIEENE"
     gene            complement(5219..6313)
                     /locus_tag="KRH39_003249"
     CDS             complement(5219..6313)
                     /locus_tag="KRH39_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG8513129.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAEEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNLFKRGDVVIGNNDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            complement(6450..7751)
                     /locus_tag="KRH39_003250"
     CDS             complement(6450..7751)
                     /locus_tag="KRH39_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8513130.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            complement(7976..8677)
                     /locus_tag="KRH39_003251"
     CDS             complement(7976..8677)
                     /locus_tag="KRH39_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8513131.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
BASE COUNT         2754 a         1613 c          923 g         3395 t
ORIGIN      
        1 aaagtaaatt ttcttatact ataataagct ttaattatag ataaataagg tttacaaaca
       61 atcaagttta aataggggtg attatttgaa tactaaacaa ttagaatatt ttatatctgt
      121 agcagaaaat ctaagtttta ccaaaacagc agaaaaattt tatatttctc aaactgctgt
      181 tacacagcaa ataaaagctt tagaagaaca aataaatgta actctattta caagaagtaa
      241 acgtcatgtt gaactaacac cagctggaaa agttttttta tctgaggcac gtactataat
      301 aaaaaatatc aatgatgcta ttgcaaaaac acaacaattt gcacatggtt tttttggaac
      361 tttgagcatt ggaaccttaa taggatatga aaaaaacaaa cttcaacaat atttaaaaga
      421 attttctaat acatacccta atatatctat ggatatttgt actaatgaaa taaccgaact
      481 tttaaactta gtaaaaaata attctatgga cctcgctttt gttattaatc cagaaaatca
      541 accattaaaa gattttgaat ataaaactgt agaaagatat tctttggtag ctcttttacc
      601 tcctggtcat ccactttaca atgaggattc tatcgattta attgaattac aacatgataa
      661 atttattttt gtaaaagaaa caggggatga gtatggacaa aagtcaatga tacagaatag
      721 atatcgtgaa gcagggtttg tacctaatgt ggtacaacgt tcaaataatc ttaatactat
      781 tgaatcattg gtatcttcta acatgggaat atccatttta ccttcatttt gtgttacaga
      841 tacaatgata gaacgtgata ttgcaatagt acctataaaa gaagtagaaa atagaattga
      901 agttgtagtt gtatggaata aaaaaaatac aaatccagca ttagaaaagt tcatttctat
      961 aatataaaat taatcaatat tagcgataaa aatagcatta accatttttt ctatagttaa
     1021 tgctattttc tactatttta ttacttaact tttaatttaa tatttataat cgttaaaaat
     1081 aactctgttt aacaaatata agcaagtcaa aaatgtgtct aaaaaagata aaaattttat
     1141 tatatacaaa tgtattatct caatatatct attttttcat tgttcatcag tctaagtaca
     1201 tgacctgtgc ttactaaaga attatcccca taagttgtat gagctaacac cgaactagca
     1261 accccaaact caactatatc ttcactcttc atattttcca aatatgaata tataattcct
     1321 gctacatatc catctccggc accaaccctg tcatatattt ctaacttata ctccctactt
     1381 tcaaaataac catcatcatt tgatatatat ccctttataa atctattatt catatcatca
     1441 aatcttctca tagttccagc tacaatacct atatcatact cttttttaat caattctgaa
     1501 ctagatttta tatttagact atctatatta agtatcttat ttatatctct ataactagca
     1561 aacagcccat gacaatatgg tagtattttc ttataatcat ttactaaatc atcataagtc
     1621 ctttctggat ttaatgattg tctaaaatta aaatcaaaga atacttttat ccccttttca
     1681 taagattttc tagctagttc taaagcaatc tctttaactg aatctgtaag ttgcatagca
     1741 ataccacaaa tatggactat atccattcct tctaaacatt tatcaatatc ataatcttgt
     1801 aatttactct cacaaaatga actatttaac ctattcaaat aagtcacttc agaaggtctt
     1861 tctccatacc cattttcaag aaaatacact cctatatgat tccctccata tgtgataaag
     1921 tcatctttta ctccaagctt tcttatattt gcacttgatg ctttacctac attattatta
     1981 ggcaactttg tcaacatata ggtattgtat ccaaagtttt taagtccaga taatatattt
     2041 aatcccgtgc ctgtaattat ataatttagt tcattggttt gtgttaagaa cttgtagtca
     2101 ggtactgaaa gtctcatcat tatttcacca aaagctaata tattcataaa tctatcccaa
     2161 taattctttc attattttat aaagtactcc tatatcttcc actttcgttt cacctgtttc
     2221 tttatcaatt attgatgtat aaacatgagg tataactttt tctacaccag catcaagtgc
     2281 aatttgaact atctctttga agttatctaa gtctatacct cctgttggtt ctaaataaaa
     2341 attatgcttt gcacaagctt ttgcaacttc tatatattca tcctttgttt taagccctcc
     2401 cattggaaaa aacttaattg agcttcctcc catatctttc atcatagcta ttgctgtttc
     2461 tataggaact ataccatctt tttcttttga acttaatgga cctgtggata tttttacaaa
     2521 tcctggttta ccagttggag aaactaatcc atttattata ctatcatttt gaccaagtaa
     2581 tgctctagac gtagccacac ctgtaaacac ttgatttata tgttgtggct gaagttcttt
     2641 agaaatctga gataccatat ttgactgatt tgggtctcca gctccaagcc ccacagatat
     2701 tgcattatct atttcctttg agtatttttt catatcttca atagctgctt ctacagtact
     2761 gtaattcttt gatagtactc ctatcaatac atgcccgtca caagcatcat atacttcttt
     2821 agcatttttt atgcttcctg caagaacatt taaacaaact ctatttttat aatattgtgg
     2881 tatacaattc atatttatcc cctccattgg taaaatttaa tttattaaat cattataatg
     2941 tcacttactc tacactctaa ctttctacta tattttttat cctattataa atagtatcta
     3001 aatcacttcc agttacactt cttatatcaa tctcaatctt gccttcatta gccttatagt
     3061 ctctagtgta tatagctatc tctccacttc taagttcatt tataatttgt tgtgttgact
     3121 tatttattat atcttcatta aagtcaattt ctccacgaag tatttctcta cctgctgaat
     3181 ccctagttat actagcactc actcccttta tagtatttat cttgtctata aatggattta
     3241 gcttgtcctc catttctttt aaagttactt tatcattact tatatatctc tcaattgcac
     3301 atgttagacc tacaatattt tcttttccta ttttcatagc tcttcctata ccctttgatt
     3361 gaagctttac attatctatg tattttttct tacctattac taaccctgag ctaggaccct
     3421 ctatagcctt agcaccactg tatatcacta ggtctgctcc caattcataa tatacattta
     3481 aatcttcttc tgctgctgca tctactatca aaggtaaatt atattcttta gcaacttgta
     3541 ctacatctaa tacacttagc atactctttt gaacacaatg atgagattta atatacataa
     3601 ttgctgctgt attttcattt atctctctta ttatatgttc cttgttacac atattagcat
     3661 aaccggcctc aacaacttct cctcctccaa gtcttatcat tacttctact ggaactccat
     3721 aatcaacatt atgtcccttt ggaattatta tttccctttt aacttgtttc ttaggattat
     3781 ttacattgta aactagactc atatcatctt ttgctatcat tgctcccaca ctctgtgcaa
     3841 tcccagcaga tgcactagaa actattagtg cactttcaca ccctaataaa tttgctatat
     3901 attcacctgt tttatttata agttcttcta ttataaagaa atttttacac ccttcaccta
     3961 ttgcttcaat aacttcctta tctactgttg atactcctag tatagtcatt ttgcctgaag
     4021 catttataac acgtcttaca gagtattttt caaatatact actcatacaa tagtttctcc
     4081 cttctactca ttctcttcta tagacttacc tatatttaaa tattctccat tgactactac
     4141 tgctcttggc tttatagacg tacttgtgat tactgactta ttcaaagaat cttgaagttc
     4201 tttttcttca ttaaccacat caaatatagt taaatcagca tcatatcctt cttttattaa
     4261 acctttattt tttaaactta ttgcatctgc tgcattttta gtaactttat ttattatatc
     4321 ttcaagtgaa tagcccatat atataaactt ttccattgtt gtagatagat tatacactgg
     4381 accatttatt ctatttttaa tatatatatc tgtacttata gtgtctggaa aaattcctgc
     4441 atccttagat ttcattgcaa tatccatact aaaactttcg gttccatgac ctacatcaag
     4501 gattatacct ctttctctag cttctatgat ttctttttta acctcattgc ctctaagtat
     4561 tccatttggc tttccattat atatatgggt taatatatct cctttttcca tatagtcaaa
     4621 tatatcctct atcgttggag gagaactccc aaagtgaacc atcataggta gattaagttc
     4681 attttttatt cttttagcta cttttaaagg ttcaacatca tttgaaatta caacactttt
     4741 actcattcta gctttgattc caacaacaaa atctttgtat tttttaactg ctcttttaag
     4801 ttcatattca tcaatattca tcatacttga aagttcatct tgagatgtta ttccctgctt
     4861 agcaatattt attaaagcat atacatttgt tttatatttt ttagtatctt cataaaattt
     4921 acctatatct aaagctcctg ttgttcctgc atctactaca gtagtaactc cactttttat
     4981 tccaacctca tctggataat ccttataaag gtctagtctt tcgtagcaat gcgtatgtat
     5041 atctatccaa ccagcactaa taaatttctc tccatctaag tttataattt gttttgcctg
     5101 atacttagaa tcatctatct gattttttac ttctattatt ttatcgtcta atatagctat
     5161 atcaattata cttttatcta ctgtttttcc acattttatt aatatgtcta tcttcacttt
     5221 actctccctc taaaataatc ttaaatttac tccaaggttc aataaagtct aaaagtatta
     5281 tttcgtcttc ctttattcta cccaccttat tttttctttc atctgtacaa tcttgaagta
     5341 tcaactgtaa ttccccttta tactttccaa agtcattatt accaatcact acatctcctc
     5401 ttttaaagag gttcttatta tcatgtttag ggaaattttc tttagcatat ttttttctta
     5461 cttctgtaga tcttgctacc tgattagtta tatctcctct tctaaagtgt tgctcattta
     5521 ccattattat cttttctact tcagaagttt tttcttcagc tactacatta aatacaactt
     5581 ggtattcatt tacttcagac attcttttta attcatcttc acttgcatat gcattcccta
     5641 caattacatc atcaatcaat cctgttgcga ataagtgctt agttgctgta gcaatatcta
     5701 agtttctatg catttcaagc gtacacagcc catcatttat attccaaggc cctattgttg
     5761 cgtattttga attaacaaaa gcagcagttc ttattccata ctttttaaat ctatttgaac
     5821 atcttacaaa aaaatcatat ggcaagcctg tacccctttg aggataaaaa ttgtgacaac
     5881 catatatatt atttacattt ggcttgtaag taattatatt atccaagtat gctacatcat
     5941 tactcatatt tagttccact tttaaatcat atggattgaa agaaatcata gcttctttac
     6001 ttccatcata tccatagtca agccttattc cactagcatt taactcacta aaaaatgtta
     6061 aatcatcata agatatttgt aatatatcaa atatgtttgg agctatatca aggattactt
     6121 caaatcctaa ttctcttgca taggttataa tttctttaaa atgtttttta acttcttcct
     6181 taggtcttgt aattgaaagc atgcacataa atattctttt aaatccgtat tttgaagcca
     6241 tttttatata tgccttatca tcttccatgt tactatgctc tggatatact gatattccta
     6301 atcttctttc catctttctc ctcctcaaat tacattattt aaaatatata aaattaaagg
     6361 caaaggtcat tcctcctacc ttcaccctta ataacttata agctttttct atattttata
     6421 tagtacaaaa cctctatatc tatgtatttt tataaatttt cactcatttc ttcttgttct
     6481 ctttgtaatt gttgtttttc aaaaactttg aagaatggat aataaacagc aaatgatatc
     6541 acaaagttga ttaatactaa gatacctgct agtatactcc agttagtact cattactgct
     6601 gctattggtg ctggcattgc aaatggtaat cttgctacca tcataggtat aacccctgta
     6661 atagtaagaa catatgatag aattgtcatt attataggtc caattataaa tggtatccct
     6721 agtattggat tcataactat tggtgctcca aatattatag gttcatttat gttgaataac
     6781 cctggtaaaa atcctaatct acctaaactt tttagatatt gtgacttaga aaacataaac
     6841 attacaacta atgcaagagt agcccctgct ccccctatcc atacatacca ctgtaaaaat
     6901 tgttctgtaa atatgtttgg cattgcatga gctgatactc cttctgtgaa tgcagttata
     6961 ttttctgcta tagacatgtc ccatataggt cttattactg gccccattat agctggacca
     7021 tgtattccaa gtacccagaa taaagttatt aggaatactg tcagtaaccc accacctaaa
     7081 ctatttcctg ataagaatcc ttttaaaggt gataacatag cgcttagtgc aacacttata
     7141 tcaaatccta aaatatgtct tattacccag aataacatta aaattactgc tgtcggtaat
     7201 aatgcagtaa atgaatttga aacctctact ggaactcctg ctggcatctt aattgtaata
     7261 ttcttttcct tcataaaacg atatatttca actgtaatta acgatgttac gatagctcca
     7321 aataatgatg gcgaattaag ttttgccata tccatccatc taccagcttc tataactcca
     7381 cctactggtt caactacttg agttggaact attgtagaaa ctaaaaatgc cataactgca
     7441 agcataccga cacctgttgt atctaaatcg tagcttttag caagtgatga tgctatccca
     7501 aaagtagcat atagagataa aaatcctaca gtatatctaa atggtatatc taatattgct
     7561 ttaaatggag ctataaaatc catataagca tcaataggta tgtttaataa tattgtgaaa
     7621 aatgacccca ctattgttag tggtaatgtt gctattatac ctgctcttac agctctcata
     7681 tgtctctgat tacttatctt cgctgctaca ggcataatct tgcgctctaa aaaacttgcg
     7741 aatttttcca tagttatccc cctgtattat tgatattatc atttatttat aatgcaataa
     7801 cgatttcata acataaacta atttttatgt aattgataat atcacttatt tattaaaaat
     7861 attataacat tataatgttt ggttgtaaac attataatgt tataatattt ttttgttttt
     7921 acaactaaac aaaaatcctg agcatattat catttcaata aactcaggat tcttattata
     7981 tttcgtattt aattacatag ttttgtactt ctgtattata tcttgcaaat gatagctcaa
     8041 ctatattatc attctcatca taggtctttc tttttcttcc taataatatg tctttagcta
     8101 gatttagagc ctttgatacc tctagttctg attcttctat aaaaaattca tcttcaaatc
     8161 tagttatatt tatatttctc ctatataagt acatatataa agaactttca ttattaaaac
     8221 tttcaacagt taaattttca tcaaaaggaa tataatgagt aaaaaatata tatggcttac
     8281 tatctaagta atacattcta gtaatcttgc tacatttttc tccaaatata ctaaatactt
     8341 gtgaatttgt atcatttatt attttttcaa ttgaaatagt ctttttagat aacttatgac
     8401 ctttagataa aagtatactt gaaaaagact ctgcttttga tagcttatta aaaagactat
     8461 tacttattac ttttgtccct tttccacttt ttttctctac atacccttct gattctaata
     8521 tctcaatagc ttttctaata gtaattttac tgactccaaa ctcttgctct aactcccttt
     8581 ctgttggaat attttcatca attttatatt cgttatttaa tattttttgc tttaatatat
     8641 ctacaacttg tatatataat ggttgatttc ttaacatgat tcacc
//
LOCUS       DAEORZ010000158         8670 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEORZ010000158 DAEORZ010000000
VERSION     DAEORZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.161"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            183..533
                     /locus_tag="KRH39_003252"
     CDS             183..533
                     /locus_tag="KRH39_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG8513132.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            552..2195
                     /locus_tag="KRH39_003253"
     CDS             552..2195
                     /locus_tag="KRH39_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBG8513133.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFTKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2276..4333
                     /gene="recG"
                     /locus_tag="KRH39_003254"
     CDS             2276..4333
                     /gene="recG"
                     /locus_tag="KRH39_003254"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBG8513134.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGITNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4392..4946
                     /gene="rsmD"
                     /locus_tag="KRH39_003255"
     CDS             4392..4946
                     /gene="rsmD"
                     /locus_tag="KRH39_003255"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBG8513135.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLGFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4948..5445
                     /gene="coaD"
                     /locus_tag="KRH39_003256"
     CDS             4948..5445
                     /gene="coaD"
                     /locus_tag="KRH39_003256"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBG8513136.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5449..5973
                     /locus_tag="KRH39_003257"
     CDS             5449..5973
                     /locus_tag="KRH39_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513137.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6317..8236
                     /locus_tag="KRH39_003258"
     CDS             6317..8236
                     /locus_tag="KRH39_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8513138.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8249..8536
                     /locus_tag="KRH39_003259"
     CDS             8249..8536
                     /locus_tag="KRH39_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8513139.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVEVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
BASE COUNT         3605 a          882 c         1597 g         2586 t
ORIGIN      
        1 attattattt ttctttgaat atatatacta tagaagaaat tttatccaaa aacttttcta
       61 ggtttaaatt ataccatatt tatagtatga tatgattata attttatata ataaataagt
      121 aaaaatagta aaaaataaat taagaaatgt ttttaataaa aataagctta agggggtcaa
      181 ccatgagtgc aaaggtaacg aatcaatatg gaaatataga aatagataat caagtaatag
      241 ctcaagtcgt atatagagct gcaatggaaa gttatggctt agtaggttta gcatataagt
      301 ctaaaggaat agtagagcta ttaaaaggcg agaacgcaac taaaggtgta agagttgagg
      361 aagtagaaga agatgccata gcaatcgaac tttatgttat aatacaatat ggtacaaata
      421 tatctactgt tgccaataat ataatagata gagtaaaata tgtagttgaa aaaatgactg
      481 gcgtaagagt tactagaatt gatattaatg tacaaggaat aagagttaag taatttcata
      541 ggaggaaaca aatgattcaa tatatagatg gtaaaaggtt aagggagatg tttatttcag
      601 gtgcaaacaa tctccaaaac aataaagagt tagttgacaa gttaaatgta ttccctgtac
      661 cagatggaga tacaggaact aatatgtcat taacaatatc ttatgcctta aaagaattag
      721 caaaagttga aaatgataat atatcagata tcggaaaagc attatctaaa ggttctttaa
      781 tgggagctag aggaaactca ggagttatac tttcacaaat aatcagaggt atagctaaat
      841 ctatagaagg aaaaagtaaa ttatcaacag aagatttagc aaaagctttt aaaaatggtt
      901 cagacacagc atataaagct gtaataaaac caattgaagg aactatactt actgtagtta
      961 gagaaagtgg agagtttgca ataaaaactg cgaaaaaaga gaaagatgta gtaaagtttt
     1021 taagtatgct tgtgaaggaa tcaaattctt cattagaaag aactccagac cttctaaaga
     1081 atctaaaaga agctggtgtg gttgactctg gtggaaaggg tcttgtactt atatatgaag
     1141 gaatgttagc ttctataaaa ggcgataaca tagaaataaa aaatgttgac ctagatacta
     1201 atatttcaac aagtatggat tttacaaaga gtactactag cacagatgat ataaaatatt
     1261 gctattgtac agaatttatt ttagaaagtt ctaaggtaga agatactaaa ataagagaca
     1321 taatgatggc ttatggtgat agcctagcag tagttggaga tgatggagtc ataaaggtac
     1381 atgtacacac taatgaccca ggaaatgtac ttcaagaagc tctaaagtac ggtcaattat
     1441 taactataaa aatagaaaat atgaagttac aacatgaaaa tacattactg gatgtagaag
     1501 aaaagaaaga aaatgatagt gaacctttag aagaagaaaa agagtttggg tttatagcta
     1561 cttctatggg tgaaggtttg gcaaatatat ttaaagattt cggagttgac catataatag
     1621 aaggtggtca aactatgaat ccaagtactg aagattttat gaatgctata aagcatataa
     1681 atgctaaaaa tatatttatt ttcccaaata acagcaatat aataatggct gctaatcagg
     1741 caaaagaatt gagtgataaa aatataatag ttatacctac aaaaaatacg cctcaaggat
     1801 ttgctgcatt agtaactttt aatggagaat taagcgaaga tgaaaataaa gaagctatga
     1861 tgaatgcttt aaactcagtt aaatcaggtc aagtaacttt tgctgttagg gatacagtga
     1921 tgaacgaaat tgatgttaaa gaaggtaata taattggaat tgcagaagga aatttattat
     1981 cagcaggaga ttatgtggat gaagtaacat caaaccttat tgaaaagttg gttgatgaag
     2041 atactgctat aataacatta ttctttggtg aagatgttac agaatctcaa gcaaatgaac
     2101 tacgtacttc tttagaagaa aagtttgaag atgtagatgt agagttatat tatggaggac
     2161 aacctcttta ttattactta atttcagttg aataatgatt ataagaacct gtatgaccaa
     2221 gtagctttta taaaattggt tgtacaggat ttttgtgtag taggtgatat atttgttgga
     2281 tttatataaa gatgttcaat atgttaaggg aataggacct aaaaaagcag ataaattaaa
     2341 taaactgggt atatttacac taaaagacct attgtattat tttccaagac aatttgaaga
     2401 tagaaataat ctaaagaaaa tagcacaatt agaagatggt gagaaagtta cgataaaagc
     2461 agtaatatca agtataaata catttagccc aaaagaaggt atgactttaa caaagattga
     2521 tgtaaaagat gagacaggct cggctaaatt agtgtttttt aataagagtt atataaaaaa
     2581 tacttttcgt ccaggagatt caatattagt ttttgggaag gttaagaaaa aatttaataa
     2641 cttagaactt acatcatgtg aattagaata tcttactaat tcacctaaga atacatgtag
     2701 atttatgcct gtgtaccagc ttacatacgg tattacaaat aaagaaatta tgagtataat
     2761 tagaacagta cttgaagata aagaattaat tatacaagaa tatatgccac aaagaattat
     2821 agaaaaatat aggctatgta gtatagattt tgcagttaga aacatacatt caccaagtag
     2881 taaggaatca ttaaaaatag ctttatatag aattgtattt gaagaattac ttatactaca
     2941 actagggttg tttgtattta aaagtggcag aaataaagaa gatggaataa agtttgaaac
     3001 aagtaaagac ttaaaaaaaa taataagtgc tttaccattt aaactgacta aagctcaaaa
     3061 cagggcatta gatgaaatta tacaagatat gaatttagag aaaataatga atagattggt
     3121 acaaggtgat gtagggagtg gtaaaacagt agtagctctc ttagcacttg caaattgtgt
     3181 tttaaatggt tatcagggcg ctttgatggc acctacagag atattagcag ggcaacatta
     3241 tatttcactt actgagtctc taaaagattt tggaataaat gtaggtctat taataggtag
     3301 tcttacaaaa aaacaaaagg atacagtatt ggaacaaata aagaataatg agattgatat
     3361 attaataggt acacatgcac taattgaaga taaggttgaa tttaataata ttggtttagt
     3421 tataacagat gaacaacata gatttggtgt tatgcaaagg agcaaacttt cattgaaagg
     3481 agcaaatcct gatatattag taatgacagc aacaccaata ccaagaactt tagcacttat
     3541 actctatgga gatttagata tttctattat agatgaactt cctccaggaa gacagccaat
     3601 agaaacaatt gctattgaaa aaagtaagcg agatagagca tataataatc ttgtaagaag
     3661 agaggttgaa tcaggaagac aagtgtacat tgtatgtccc ttggtagaag aaagtgaagc
     3721 catagaagcc aaatcagcag ttgaacttgt tgaggagtta agagcggaat attttcacga
     3781 cttaaggctt gggttgcttc atggtaaaat gaagtcaagt gaaaaagatg aagtaatgag
     3841 actttttaaa aataaagaaa tagatatttt agtttcaaca acagtaatag aagttggtgt
     3901 caatgtgcca aatgcaacat taatgataat agaaaatgca gaaagatttg gacttgcaca
     3961 attacatcag cttagagggc gagttggaag aggtagtcat aaatcgtatt gtgtactaat
     4021 ttatgattct aaaacagatg tatgtagaca aagaatggct ataatggaag agaccaatga
     4081 tggttttaag atttcagaga aggatttaga aataagagga cctggggaat tttttgggac
     4141 aaggcagcat ggtcttccag aacttaaggt agcaaactta tttaaacata taaaaatact
     4201 gaagttagca cagcaagaag ctcgatatat attgggtgaa gataataatc tacaattaaa
     4261 agaaaacatg gcactcaaaa aagaaattat agataaattt aaagatacat tgaaagaaat
     4321 ttcattaaat taattttaaa ttgtgttaaa atattactaa tgtaataatg tgctgaaggg
     4381 agattgtcat attgagagta atttcaggaa aagcgagagg attaaaacta aatactccaa
     4441 aaaatgaaga tgtaagacca actacagata gagtaaaaga gtctctattt aatatgataa
     4501 attcatatat aatggaaagt gaagttctgg atttatttgc tgggactggt tctttaggaa
     4561 tagagtgctt atctaggggt gcaaagtcat gtacttttgt agatatcagt aaggaaagca
     4621 tagatattgt taagtccaat ataaaaaagg caagagttga aagtgaaagt attattttaa
     4681 accttggttt taagactgca atagataaac ttaaattaca aaatagtaaa tttgacataa
     4741 tttttatgga ccctccgtat tataaaaata tgtttataga agctattgaa aaaattgata
     4801 attctaatct attgaacgaa gatggaataa tagttgtaga acatgatacg aatgatttat
     4861 ttccagataa gatatgtaaa ttggagaaaa ctagagataa gaaatatgga aatactacat
     4921 taacttttta taagatggag gcataatatg gaaaataaac ctagaaaagc tatatttgca
     4981 ggaagttttg acccaattac taatggacat ttagatatta tttgcagggc ttcgaagtta
     5041 tttgacgaat tacaaatagg agtattaaat aatcctaata aaaagggtct atttagcttt
     5101 gatgaaagag taaaacttat agaaaaaagt acaagccatt taaataatat aaaagttgtg
     5161 acttttgatg gtttattaat aaattactgt caggaaaatg ggattggagc attagttaga
     5221 ggtgtgagaa gtggtgctga tgttgattat gaacttcaga tggcgcatat gaatagagag
     5281 cttaatccag atatagagac tataatttta ccaagttgta caaaatattc atttataagt
     5341 tcatctttaa taaaagaggt tttgctcttt gatgctgata taaaaaattt ggttccaaag
     5401 attgttttgg aagaattaaa gaaaaaaact agtgggggta attgatatat gaagatagat
     5461 ttagaaatga ttgaactatt tgagcagtta gaggagatat ttagaagtgc ttctacaatc
     5521 cctttctcac ataaatgcac agtggataaa gatgaggttt tagcaataat taatgatata
     5581 agaactttaa taccagaaga agtaacacag gcagtatgga ttaataaaga aagaaataaa
     5641 ataataagtc aagctaagca agatgctaca aatatagttg aacaagcgaa aaaagaagct
     5701 gagagaatac aacaagaata tcaagataat atagaggaat taaagaaaaa ttctgaagat
     5761 gtagtaaagg catatgtaga atccagtgaa cctgttgttc aagcagacca aagagcaaaa
     5821 gatatagttg atagagctga gagaatagcc aatgagataa gaattgggtc aattgaatat
     5881 gcagaggatg tgctttctag tgttgagtat aatttaagag aaatattaga agaagtaaaa
     5941 agagataaag ctgaacttag acctaaaaaa taaattttaa ataaataaaa atggagctat
     6001 tacacaaaaa attccaattt atgtgtgata gctctctttt aatatagcaa ttttttgtta
     6061 tataattgtg ctttttagat tacttatatc ttattttgat attataatgt agtttaatct
     6121 tttatttgtt gaaatcaact tatttatttg tataaataat ataggttatt ttcaaatttt
     6181 tttaataatt ttaccactgt cttcttttag attagagtta gactgtgcaa aattataaaa
     6241 taatactaaa tcatgtgcaa ttatttggta tggcactaca tgtagaaaat atagataata
     6301 aaattaagga ggtagtatga aggaaagggc taaaaaaata ttagatttta ttattaaaaa
     6361 taacaatgta acatcacaag aactacaaga aaagtttaat gtttcaaaga gaactattta
     6421 ttacgatatt ctagctataa atgaacaact aggaaaaagt ggtaatataa aaaatgtaaa
     6481 acacaaattt atatttgaag gtaatttatg tgatgcaagg aaaataattt caactgaaga
     6541 agataaattt ttagatagtg actacagaaa aacttttata ttaaacaaga tattacttgg
     6601 cgaaaaaata tcaatagaaa aactaacaaa tgaaatgtta ctttcaaaaa acacagtagt
     6661 acaaacaata actgatgtaa aaaaatacct tcaaacaatg gggttaagat tagagtataa
     6721 gggaaagtac aaaattatag gtgatgaata cgttattaga gagctgtttc tgataattgt
     6781 tcaagaaaat gtactggaaa ttaattctat aagtgaggaa gtaagtagtt tcgatacaaa
     6841 aggacatata aaacttactg attattcttt attaaatcta actaagtttg tggaatttct
     6901 aaataaaagg ataagagatg ggaaaacatt atactcatat aaatatctaa atgaagctaa
     6961 aaaaattagt tactttagta attgtaaaga acttttatgt gaagaagcta atgaaaatga
     7021 acaagcctat atatgcactt acataagctc acttcccagc ttaaatagtg aagttaagga
     7081 agatgttgtt gaagaatatg tagataaact tatagataag tttgaagtaa atacagcaat
     7141 aaaattagaa agtaagcatg agtttaagaa aaatatttta aggcacctcc actcttctta
     7201 caacagaata cgatttaagt ttcctattag aaatccaatg ttagatgaga ctaagtataa
     7261 acatgagtca ctttataaaa ttataaaatc aattatagag aatgaagaag agtttcctgt
     7321 atttgagggg ataagagaag aagaaatagg atttatagca gcgtattttg gaggatattt
     7381 aagagggtct agggataatg gtctaagaag aaataaagtc ttactagttt gtcctaatgg
     7441 tcttatggta tcaaagagtt tggaaataca actatataaa tacataccaa ccatagaaat
     7501 agtaggtata gtatccatta agcaactaaa agaagtaaat gtatactacg attacataat
     7561 tacaactata gatatacaaa atgtaaacaa cgtaattgtt gtaaatcctc ttttaacatc
     7621 atcagatgtt caattactta tgaacaaact aataagtgta aaagaaaacg agaagtattt
     7681 caatcttgaa ttaataatac aagctataag aaaaaatgga gttatcaata atgaagaagc
     7741 actaaaagct gatttattaa acataataca caagatagat gaaggagaga tgtatcaacc
     7801 aatgttaaag gaattaatta atgcagaaag ggtcaatata ataaaaaatg ttagagattg
     7861 gaaagaagca ataaaaatag cctcaaagcc attgttagaa gataactcta tagaagagtt
     7921 atatatagaa aatatgataa agtcagttga gaaatatgga ccatatattg ttttagctga
     7981 tagatttgct ctaccacatg caagttctaa agaaggagta aacaagcttg ctatgtcttt
     8041 actaatagta gaagatgaag ttgatttatt aggaaaacca gtaaatatat ttatggtatt
     8101 agctgctgta gataacacta ctcacattag agcattagca tcactatcag agatgatgta
     8161 cgaggaagaa aatgtaaaac taataattaa tggtgataaa agttcaatac tagaacttat
     8221 aaataagcaa aattaggagg aatgaaaaat gaagatatta gcagtatgtg gttttggtgt
     8281 aggttcatca atggtattaa aaatgacaat acaaaaggct ctaaagcagt taggtataga
     8341 atgtgaagta gaaaatacag atataaattc agctagaggt gctgattgtg actgtatatt
     8401 cacaagtcaa gaattgttaa atgaattaaa aggaacttgt aatgtagagg tatatccagt
     8461 taaaaagtac atggatttag cagaagtcac agaagttgtt caaacttttt taagtgataa
     8521 aaatgataag aaatagtata agttttaaaa agtaaattta agttaatcag aataaaataa
     8581 aacttactaa gttcaaaaat agtaagaaag agaggaggga tttagtggta gaaaacttaa
     8641 acagttgacc tatcactaaa aaatataatg
//
LOCUS       DAEORZ010000159         8623 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEORZ010000159 DAEORZ010000000
VERSION     DAEORZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.162"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            99..1382
                     /locus_tag="KRH39_003260"
     CDS             99..1382
                     /locus_tag="KRH39_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBG8513140.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(1460..1975)
                     /locus_tag="KRH39_003261"
     CDS             complement(1460..1975)
                     /locus_tag="KRH39_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG8513141.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(1975..3351)
                     /locus_tag="KRH39_003262"
     CDS             complement(1975..3351)
                     /locus_tag="KRH39_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG8513142.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(3376..3927)
                     /locus_tag="KRH39_003263"
     CDS             complement(3376..3927)
                     /locus_tag="KRH39_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG8513143.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(3985..4200)
                     /locus_tag="KRH39_003264"
     CDS             complement(3985..4200)
                     /locus_tag="KRH39_003264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513144.1"
                     /translation="MQIHRELSKSENEYMCTKKEEQRYSKCEICGVLIDDTDKVYNNT
                     YNLCKKCKRNISTEKIVKYINIYDESK"
     gene            complement(4169..5002)
                     /gene="fdhD"
                     /locus_tag="KRH39_003265"
     CDS             complement(4169..5002)
                     /gene="fdhD"
                     /locus_tag="KRH39_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBG8513145.1"
                     /translation="MNNIYSKTKLQENGKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYINTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(5051..7195)
                     /gene="fdhF"
                     /locus_tag="KRH39_003266"
     CDS             complement(5051..7195)
                     /gene="fdhF"
                     /locus_tag="KRH39_003266"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6779..6781),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBG8513146.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(7446..8504)
                     /locus_tag="KRH39_003267"
     CDS             complement(7446..8504)
                     /locus_tag="KRH39_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG8513147.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
BASE COUNT         2696 a         1568 c         1000 g         3359 t
ORIGIN      
        1 ccaaattata tttaaatagt taggctatcc aaaactaata tataaaatta gaataattag
       61 attaatatct acaaatcata acctaaagga gagataccat gaatacatta ctaaacaata
      121 aatttagcat taaaaacttt tcaaatgctt gggttatagt acttggttgt atgttaatac
      181 aagctgttcc atttagtatt gtagcaagta tacagcctca atttataagt tatgttgtag
      241 aaggagaagg ctttagttta gcttcttttt ctctaatatt cactattggt actattgcct
      301 cagcaattgc atcacctttt ataggtgtat tattttctaa agtcaatatt aagatactat
      361 atttattagg ttgtataatt tctagtattg cttttgctac tttttctata tgtactgaac
      421 tatggcaatt ttacactgta tcatgtattg ttcaaattgg cgctacaatt atatctgcta
      481 taggaatacc tgttttaata agtgcttggt ttgatgaaga aagcaaagga aaagctttgg
      541 gctttgcttt tgctggtggt tctattggta gtatatttct acaaccactt gcagttcgct
      601 tattagcaac tcaaggatac agtcgttctt atctaatatt tggaattata gcattaatag
      661 ttggtatacc tgtaatatta tttatactca ggatgcctaa agatattaat gaaaaggtaa
      721 aatctaaagg taaaaaagag aaagcatctg atacaatttc tccttcagta tggggttata
      781 cattaaaaga agctaaaaaa attaaatatt tttggattat gactattgga tttatatttg
      841 ttggtatata tgtttcatca ttatctgtac aatatccaac ttatcttaga aattatctaa
      901 atcctattct aataggaaca gtaggctcca catttgcttt atttgctctc attggtaatt
      961 tatttggagg attattgttt gataaattcg gtcaaacaaa gccacttatt atatcttttg
     1021 tattggttct tatagcaact ttatcattac tattggtaga taaatcttct acttttgcat
     1081 ttgtatttgc cattttgaaa ggtctttctg tatttgctta tatgattggt cctgcttatt
     1141 taactggctc tttctttggt caaaaggagt ttggtagcat atatggaatc gtacaattaa
     1201 tttttgcagt gggatttgca ttaggttcaa cattatttgg attgttagtt gataattttg
     1261 gatacagatt ttcttggtct attataattt tatcagttat aattgcctat actttactta
     1321 ttataacttc tataggaatg aaaaaattga ataaagaaag attagataaa ctaatgcaat
     1381 aattgatttt tagatatata aatattataa taaaaaagat ggagctttta atcataaata
     1441 aaactccatc ttttttatat tacaatatag ctaattttct cttattttta actttactat
     1501 ctatatcctc atccttaact atagtaaatg ccttagttgg acaaacattt acacatgcag
     1561 gcccttctgg cgattctata caaaaatcac acttatttgc tgtcattctt tctctaaagc
     1621 aaagctttcc ttcatctaca tcttttaatg taactatgtc tattgcacct ataggacatg
     1681 ctaacataca agtcttacat cctatacaat tttcttcatt tatttttatt gtgttaccaa
     1741 ccctaactat accaccattt ggacatgcct ttgcacaagg agcatcttca caatgtctac
     1801 actgtattgg agcacttact tcttttgtct ttatgacaga aagttttgga ttaaaattta
     1861 tttcatctgg attttggtaa aaaatatctt catctgaatg tgcaactaca catcctatca
     1921 tacaagttct acatcctatg catttatcag ggtctgctat aacaaaatag ttcatcactt
     1981 agccacttcc tttcctatac aatatgtagt gtgtaataga tgatgagatt tttctcctaa
     2041 tggttctacc agatattcat catacagttt tttaatatca ggattctcat gagattttct
     2101 aattgctaaa tgttcatcat gcacgtatgt tgcttttgtt ctattttcat aagccaattc
     2161 cttatattct tctagtaaaa tttttggttg acctccacca cttacacacc caacaggaca
     2221 agtcataact tcaataaaat ctacatctaa ttttcctttt tccaagtctt caagcactgg
     2281 tattacattt tttaatccac ttactactcc aactcttaag tctaagtctc ctattttaat
     2341 agttgacttt ctaaaaccat tttctcctct tacttgtttt acttctacat caggaatact
     2401 ttctccagtt ataagctcat atcctgttct tatagcagct tccataaccc caccagtaac
     2461 accaaagata gttccagctc cactataact acctaaagga ctatcaaatt tttcttctct
     2521 taaattttta aaatcaattc ccatgtcttt tattaaataa gctagttctc tagtagttaa
     2581 tactacatct acatctctat atccactaga atccatttct tctctatcac attcatattt
     2641 tttacatgta catggcatta tagaaacact aaatatcttt gatgcatcta cattatttat
     2701 ctctgcacca taagttttaa atattgcacc acccatctgc tgaggtgatt tacaagaaga
     2761 taaatgatta gttaatttag gataattttt ttccatgaat tttacccaag ctggacaaca
     2821 agatgtaaac attggaagag ttccaccttc tgtcactctt ttaattaatt cactaccttc
     2881 ttccattata gttaaatctg ctgcaaaatt agtatcgtag actctatcaa aatttaaagc
     2941 tctaagtgct gctgccattt tcccagcacc taagtatcct aaataatatc caaaatcttc
     3001 tcctaaagca actctaactg ctggtgcaca ttggaccata gtgaatacat ttgggtcact
     3061 taaagctttc ttcaccttat ctatatcaca tacattgtat gctgcaaata taggttcatt
     3121 tatagactct ggtatctctc tttcttgttt tttctcttgt aaaaactcaa atccttcatc
     3181 tataactgag gcataagatt tacaagtttg aacacattga ccgcacataa cacatctatc
     3241 taaatttatc tcttgtggct ttccctcttc accttcaata gcatttactg gacagacctt
     3301 tgaacattct ctacatccag tacataaatc cttatctata tttattatca taaacgtcac
     3361 ctacccctgt atcctttatt tatttgttaa caataaacta agtgcagctt gtctattctt
     3421 agcttttttg tcatctattg gagtaacaag ttttaatgct tcctgaggac atgcacttat
     3481 acaagctatt ttttcattgt ctttacataa gtcacattta taagctatct tcttattttc
     3541 atctaaaatt acttgttcta cttctttacc atcttcatat tgtggaagta agtctatagc
     3601 tccaaaagga catgctagca gacaagtttt acatcctata cacttttctt catttacaga
     3661 cataatattg ttttctttaa ctatagcttt ttgtggacat gagtttaaac aaggagcatc
     3721 ttcacaatgt ctacactgta ttggcataca gaaattgtca ccttttacta agtaaagtct
     3781 aggtataaca ggtatatcaa ctgttccaac tgtataaccc acattattat gctgattatg
     3841 cattgtaaaa caagctactt cacaagctct acaacctgta catttgtttg ggtctgctat
     3901 tacaaaagag cctattttat ttttcatatt atcccacctt tcaatttgta tatttccaac
     3961 cctctagtta ttttcatcat atgtctactt actttcatca tatatattta tatatttcac
     4021 tattttctca gtacttatat ttcgtttaca ttttttacac aaattgtatg tgttattata
     4081 caccttatca gtatcatcaa ttaatactcc acagatttca cactttgaat atctttgctc
     4141 ttctttttta gtacacatat attcattctc actttttgat aactctctgt ggatttgcat
     4201 aaatattcat ccgtcttcct cttacaaatc caataagcgt tatatttaat tttttagaaa
     4261 gctctattga taaattagtc ggtgctgatt ttgatactac aataggaatt tgagtcatag
     4321 ctgcttttaa tatcatttcc aatgatattc ttccacttac agccacaatt ttatctttta
     4381 atgttatgtc atttaaaaca caaaatccaa tagccttatc cattgcatta tgccttgcta
     4441 catcttcaca agtgactatt tttttatcaa aattaaatac agatacgcta tgtaccccac
     4501 cagtattttt aaataattct gatgaattta aatttctatc cattatactg taaatcgtat
     4561 cataatttat ttttatattg ctctttacag gagcacatgt tatatagtct agtgaattta
     4621 aaaatataat ttgctcatct tgattttttt gtttctcttg aatagttact tttgcaaaac
     4681 gttcactctc atttatctca aattttaata agcttttttt gttttctatg tatccttttg
     4741 ttttcaagaa tcctactata agttcctcta aattatatgg ggtacataaa aatgtattta
     4801 tatatttatc atttaaaata aaactaagtg gatattctgc aattatttct tcattatcta
     4861 ttgatacttc atgttcattt attttgctta cttctacatt aaaagaattt aaatatttgt
     4921 agtccactag actataacta ttttcaaaaa ctttatcctt accattttct tgtaatttag
     4981 tttttgagta tatattgttc ataaaatatt agcctttctg ccttttataa atcttaaatc
     5041 taataaatca ttagatattt gtagctgtca ttttgtcttt caaactttga tactcttctc
     5101 ttacacattt ttctgctaat tcttggtctt caagtttttc aagtttaaca gcacaatatt
     5161 tatactctgg tgttttagat atagggtcta aatttgctat tgtaagttca ttgcaagctc
     5221 caacccacca ctgataagtc atatatgttg caccttcctt aactctatca gtaactgttg
     5281 ctcttgttat tacacttcct cttcttgaac taattctaac taattcatca tcttcaattc
     5341 ctaatttttc agcatcatta gaatttattt gaacacgtcc tggttcatct tctaatgaac
     5401 taagtgttct acaattacca gtcattgttc ttactgaata gtgtccaact tctcttacag
     5461 tacataaact aaatgggtat tcatcatctt caacttccat agggggtctc cattcagcag
     5521 cgaacaaatt acctttacca ttaggagtag aaaacttttg accttcatac agatacatag
     5581 tccctttatc ttccatactt tcactcttac aaggccattg aacgcatcct tgagtttcta
     5641 tcttttcata agtagcacct aagaaacttg gacataattg tctaagttcg tcccatattt
     5701 ccttagtatt tttataattc ataggatatc ccattgctgt agaaatttcg cttataattt
     5761 gccagtctgg ttttacatct ccttgtggct ctatagcttt tctcatcaat tggaatcctc
     5821 tatctgcaca tgtatatact ccctcatgtt ctccccaaga agtagctgga agtataacat
     5881 cagcgtgaag tgctgtctta ttcataaata tatcttgaac aatgacaaat tccaattcat
     5941 ctaatgcttc tctaacttct gatgcatctg ggtcactttg cactgggtct tctccaaata
     6001 tataataagc tttaagcttt ttctctttta gtactaagtc tggaacttga gttaaactat
     6061 aaccattatt aggagataat tttacccccc aagctttttc aaacttttct cttatcttat
     6121 catcagttac attttgatag ccaggatata cattaggaag agctcccata tcacaagctc
     6181 cttgaacatt attttgtcca cgtacaggtc ctattccaac gctttctcta ccaaaattac
     6241 ctgtaagtag agcaattgat gcaagtccct taactacatc aacagcttga ccaaattgac
     6301 agacacccat tccataaagt atcatagctt ttttaccttt tgcatattct ctcatagctt
     6361 ttcttataag ctcttctggg attcctgtaa tcttttcagc atatttagct gtatatggtt
     6421 taacaatttc tttatactca tcaaatcctt gtgtatgatt ttgaacaaat tctttattgt
     6481 aaagaccttc ttctattaat acatttccaa aagcatttac taaaaccatg tttgttccac
     6541 cttttatagg tagatgtata tcagcaattc ttgcagattc agttactcta gggtcagtaa
     6601 caattaattt agcaccattt tttttagctt ttactattct atttgccact attggatgtg
     6661 aatctgctcc attatatcca aatataaata aaacatctgc attttctatc tctggtatag
     6721 aattagacat tgcaccacta cctaatgagt aagccagacc ggctacagat ggtgcatgtc
     6781 agacacgagc acaatggtca acattattag ttcctattgt agctctcata aatttttgca
     6841 ttatatagtt tgcttcatta ccaggtcctc ttgcagaacc tgttcccata atagaatcag
     6901 gtccatattt ttctttaatt tcatttagtc ttgaagctgt atagtttata gcctcatccc
     6961 attcaacctc ttctagtaca ccttgttttc ttatcattgg cttttttagc ctaggtgtta
     7021 gaattttagg gtcatttaga aaatcccatc cataacgacc ttttaaacac aaactttctt
     7081 gatttgttct accattagcc ggttcagctc ttaatatctt attatctttt actactagat
     7141 ataattggca tccagctcca cagtatggac aaactgttaa aatttttttc tccatacaat
     7201 tcccccctta gatttatccc ctgataagta aaatttatct taaagataat gagtgttttt
     7261 ataatatgca aaatcataaa tattttgcaa tactatctat taaattcaac aattttctca
     7321 caattcctct taaatttcaa aaaaaatatg tcataataat ctttatattt caaccattta
     7381 attaaaatat tctagttctt atgacatatt ttttaatata ctcaatatat attctttaaa
     7441 cttatttagt tttcacttaa aacaatattt ctgccaattc tatttttcat tctatagtga
     7501 gtaattacag taaatattcc acttccaatc agaatcaaat agtacataaa tccaccatat
     7561 aaaaatactg catatcccat caaaggttga gggaatactg atttgaatat agtgaaaaag
     7621 gctaattcat tagctcctac attcccaggt gttggtattg gagatattgc catatataaa
     7681 aatgcttgta atgtcagtat ataaatatag ctataacctt gtaaattaaa tgctttatat
     7741 atccaaaatg aaacactaaa gtaagcactt aattgaataa aagttactat tatagttgaa
     7801 attaatacct ttttattctt tgcaaaaaaa tttattgctt tactataatc atctataaaa
     7861 ctttctatgg attcaatctt attatctaaa aattttaaaa acttaatttt tgataaatgc
     7921 tttatagtaa ttctcataaa atattttatt tttttaggat ttaaaataac taatattatc
     7981 ataattaaga caaatgaatt aatggctatc cctagaaaca ctagaggcat aatcattttc
     8041 atttgttttt ctaacatagc aaaattcatt aaaacaagta ttgtacaata aaaagtaact
     8101 actatttgga agataataga cttattggta acaactgcac ttgccttact aagtggcatt
     8161 ttacattttt taagtacata tatctgtact ggttggcttc ctgaagcaaa aggagttatt
     8221 aaattataat aaaatcccat aatggctagc ttaaaaccaa taaatctaat attaactttg
     8281 tgagtgcttt ctataattat cttcattact actccttcaa gcattatgtg acacattatt
     8341 gccaaagcac ctatgaataa aaacttctta tctaccataa ccataacatt tgatagcatt
     8401 ttaatatcca atgttttaaa cactaggtaa attgttatac ctattaacaa aaataaaaat
     8461 gtatactgta gtaaactctt tctaatattc tttagtctat ccattagaaa taaccatcct
     8521 ttgctcaaga tttatactaa atactatatt attagtatac actatgaatc ttaatatttt
     8581 ataacaaaat accttacaat tttgtcattt acaaaaaata tta
//
LOCUS       DAEORZ010000160         8464 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEORZ010000160 DAEORZ010000000
VERSION     DAEORZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.163"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            203..1189
                     /locus_tag="KRH39_003268"
     CDS             203..1189
                     /locus_tag="KRH39_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG8513148.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1358..1504
                     /locus_tag="KRH39_003269"
                     /pseudo
     CDS             1358..1504
                     /locus_tag="KRH39_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1526..3007
                     /locus_tag="KRH39_003270"
     CDS             1526..3007
                     /locus_tag="KRH39_003270"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBG8513149.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENNENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3033..4004
                     /locus_tag="KRH39_003271"
     CDS             3033..4004
                     /locus_tag="KRH39_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBG8513150.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4260..4862)
                     /locus_tag="KRH39_003272"
     CDS             complement(4260..4862)
                     /locus_tag="KRH39_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8513151.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIEM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            5143..6354
                     /locus_tag="KRH39_003273"
     CDS             5143..6354
                     /locus_tag="KRH39_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBG8513152.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKYNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6422..6619
                     /locus_tag="KRH39_003274"
     CDS             6422..6619
                     /locus_tag="KRH39_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBG8513153.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6653..7624
                     /locus_tag="KRH39_003275"
     CDS             6653..7624
                     /locus_tag="KRH39_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8513154.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7811..8188
                     /locus_tag="KRH39_003276"
     CDS             7811..8188
                     /locus_tag="KRH39_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8513155.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3445 a          883 c         1354 g         2782 t
ORIGIN      
        1 atagtagata aaattgaaat taaatataac aacaaaaaat gaaaattaca attaatttaa
       61 tgttaaagac tcaattcttt ggatgtggat atggatagta caagtagtat ataaaaagta
      121 ttgactggac aaataactat attttatttt aaatatataa tataatgtaa taaaatatat
      181 caaatgtaga aggtgataac ctatgaaaaa aataacaata aatgatattg ctaacttggc
      241 aggagtatct aaaagtactg tatcaagata tctaaataat aaagatatca gtgattctac
      301 taaagagaaa ataaagacaa taatagatga atatggatat gagccgaatg catttgctca
      361 aagtttgaga gctaaaaaaa catactttat tggtattata acaccgtgtt tagattcttt
      421 tgtaaaatct aaaattatga tggcaataga tgaagaactc aaagaattaa aatatacatc
      481 actaattatt aatactaata gaaaaataag gtcagaaata gatagtatat caaagttagc
      541 tagtctaaaa gttgatggaa taatactaat tggtaccgaa ataactaaag aacataagaa
      601 tgaaattgaa aagttagata taccgatagt tgtagttgga caaaaagttg atggtatcaa
      661 tagcatagtg aatgatgatt atggagcagg atataaaatg ggtcaatata ttgctaataa
      721 aggttacaaa aacattgtat atttaggtgt tgatgaaagt gatatatcag ttggtttaaa
      781 tagaaaaaat ggagtactta atgggttaaa agataaaggg tatgatgcaa aagtatttta
      841 tactgacttt gaccaagaaa cgtctataca aagaagtgga gaaatgctag aaagtgaaaa
      901 tccagatata ataatatgtg caactgataa tatagcaata gcaacaatga aagaaatcaa
      961 taagagaggt aaaaatatac cacaagatat atctgtagct ggatttgggg gatatgatgt
     1021 attgtctatt ataagcccta aacttacaac aataaagttt gaaaataaaa atgcaggtaa
     1081 agtagcagca aatacgatag taaacttaat acaagaaaga aaagaaccac tattaaagga
     1141 gattaaattt gaattaatag aaggtgaaag tacaataaat aaaaattaag atacacatta
     1201 catatattag taatgtgtat ttttttacta aaaaaactat aattctaaaa attaggaaaa
     1261 cttttttgaa agaaatattg catctgaaat aaatttatgc taatctttaa ttaaaccggt
     1321 ttcgaaaccg gtttaattaa accgatagaa tagcaaaatg agtgcaaaaa taactgattg
     1381 taacaaagta tcaaagtaat tattagagta cataggaaat aaataaaata taaaaggtgt
     1441 agcacattgt gtaacaagat taagaattgt tttagatagt aataataaag caaatataaa
     1501 attataaaag aaaaggaata aaaatatgta caaagagcct aaatatagaa ccatattaga
     1561 atcgacaaaa gaagaattaa ataaattaag aaatatatct ttaaatgata aatataaacc
     1621 actatttcat atacatccac aacatggact acttaatgac ccaaatggat tagcatatta
     1681 taatggtaag taccatgtat tttatcaatg gtatccatat gatgcaactc atggaatgaa
     1741 gcattgggct tatgtaagtt ctgatgattt tgtaaactgg aatagagaag atgtagcatt
     1801 aataccaatc gaaagttatg aatctcatgg agcatattca ggtaatgcga tagaggtaga
     1861 tggaaaattg catatgtatt atacagggaa tataaaatat agtgcagaag atagatatgc
     1921 atatcaaaac ttagctataa tgaacaagga tggtaaaata actaaatatg aaaataatcc
     1981 aatagtaagt gaaataccaa aaggatatac aggacatgta agagacccaa aagtatttaa
     2041 aagaaaggat aaatacttta tgttacttgg agcacaaaca agtgataaaa aaggagttat
     2101 aatagtatat gaatctaaaa actctataga ttggaatttt aaaggagaat taaatgtaaa
     2161 aaatatagat gaagattttg gttacatgtg ggaatgtcca gattatatta atatagatga
     2221 aaaagatata ttaatatttt caccacaagg agtagaacct aaaggatttg attatcaaaa
     2281 tatatataat gtagtatatg ctattggtaa tatggattta gataatttaa catttgaaat
     2341 agatacaatg aaagaactgg aaaaaggatt tgatttctat gctccacaaa catttataaa
     2401 ggactcacaa ataatactat ttgcatgggc tggaatggga gaagttttat accctacaga
     2461 taaaaacaaa tgggcacatt gtctaactgt tccaagaaaa ttaaatataa aaaataataa
     2521 gttattgcaa atgcctgtag atgaattaat taaattaaga tatgatgaaa caagtgggca
     2581 aaatactata aagaataata tcaatattat agaaaataat gaaaatgtat atgagcttaa
     2641 tattaatata aaaaatattg acagcaataa atttgggctg gaattatttt catcacaaga
     2701 tgaaggtgta aaattagaat ttaacaaatt aggaaacatt gtaactttag acagaagcaa
     2761 ctttaaaaaa gttttcggtg ttgaatatgg aacaaataga aaagaatata taaatattga
     2821 tgaaaatact aatatcaaag tactagcgga tagaagtatt ttagaaattt ttataaatga
     2881 tggagaagta gtatttacta gtagaatatt tgctaaagaa aattctaatc aaataagggt
     2941 ttatagtgat aaaatagtag agtatgagta tactaaattt aaattaaagc aaggtattga
     3001 attataaaaa cacattataa ggggtagaaa agatgaaaaa agtaattagc ataggagaag
     3061 ctttaataga ctttataccg aatcaaaatg gtggtaaatt aaaaaatgta tcagaattta
     3121 gaagagtagc aggaggagct ccagcaaatg ttagtgctgt tgtagcaaag cttggaggaa
     3181 aatcaagttt tatatctaag ttaggaaaag atgcatttgg tgattatata atagatgtat
     3241 taaatgaagt aaatgtaaat actgattatg tattgagaac aagtaaagca aatacaggac
     3301 ttgcttttgt atcattaaaa gaagatggta atcgtgattt ctcattttat agaaatccaa
     3361 gtgcagatat gcttttagaa gctgatgaag ttaaaaaaga gtggtttaat aattgtcata
     3421 tcctacattt ttgttcagta gatttaatag atagtccaat gaaattagca cataaaaaag
     3481 caatagaata cgcacttgaa agtaatagta taataagctt tgaccctaat ataagattac
     3541 ctttatggga tagtgagcaa tcttgtaaaa aggcaatatc agaattttta ccttttgctc
     3601 atattgttaa gatatctgat gaagaattag aatttataac aggagaaaat gatatagaaa
     3661 aatcattaca taaactcttt gtggggaatg ttgaactagt attatataca aaaggaaaag
     3721 atggagtaga tgcatacact aaaaaagtaa aaggtatgtg taaaggggtt aaagtaaatg
     3781 caatagatac aaccggagca ggagattctt atataggttc atttttatac accttattgt
     3841 ataaacaaat aacattgaat gatataaaag aaatgaaaca agaaacgtta aatgagtatt
     3901 tagaattttc taattattat gcagctcgta gtacaactat taaaggtgcg ataagttcat
     3961 atgcaacaaa ggaagaaata actgaattta taaaaaattt atagtaggaa tgagtaatag
     4021 gttttgaagg aagtaacatg taaagagttt ttaaggatgt ttactatgaa aataattaac
     4081 atataaaaag caataaagag gctgtctcaa aatagagata agcctcttta taattgttga
     4141 aatgataaaa aatattttaa ttattgttac tttttagaag aatatatatc ttattttaaa
     4201 taagcaaatt atgtaagatt tatttatatt atcaatactt tacttggagt attatgtctt
     4261 tagcagttat ctacttcaat tgaattatct aaaatataat aaatttgttt tgcaaattga
     4321 cgagaatgtt cttctttttc tttattcata aaattaggtt tacctaaatc acgttcaaca
     4381 agcatttcta tatattttaa ctgtgaatgc ttacagtagc gcttcatacc attttcccaa
     4441 atatcagcac ccttttcagg tctatatcca catgttgtaa taagtgctac tggttttcct
     4501 gcccatagag cggggccttt ctcattacca tagtacttat tcattccata caccaatctg
     4561 tcaagtagtg ctttcattgg agaagtgcag taccaagaat aaattggagt agcaaaaaca
     4621 attaaatcac acataagaat tttcttgaaa attttctgaa catcatcttt acaataacat
     4681 ccaaaatcaa ataagttatc ctgacatttt ttacaagcaa tacatggctc tattttacaa
     4741 tcatataacc atattacttc aatatttgaa ttataatttt ctaattcctc aataaaaggt
     4801 tttaaaatag cagcagtatt accttttttc ttaggacttc ccataagaat gcatacttcc
     4861 atattgatac ctccacagta ttttaaaata gtttttagtg cttaatatag ttttaggata
     4921 ctatgagaat acgttaacaa caatacactt tgtatatttg taataaaatt gaaacaaatt
     4981 gttttatcaa taaaagttgg aatgtatcta taattaaaaa tattttaggt atgtttttac
     5041 atatatgtgt gtagttaact atggtaattt attgtaaaat tatcttatag gattataatt
     5101 gcttaattta tgattatatt taaaatcttg gaggtaaaaa atatgtactc aaatgaagac
     5161 tataaattta tagaagaaaa tgcaccatgt tatatatatg atgagactaa tataattgaa
     5221 aaatgtaagg aattgaaaaa taatatttta ggttttgatt ttttatattc tataaaaaca
     5281 aatccatata acaaaattgt aaaaagtatt gcagagcaag gttttggagc agatgcttct
     5341 tcaactaatg aagttatgat ttcatcagaa tgtggagttt ctaaagatga tatttattat
     5401 tcatcaccag gcaaaacaga gattgatata gaaactgttt tagataaatg cataattatt
     5461 gcagatagta ttacagaaat agaacttata aataagatat ctaaaaagta taatcagaaa
     5521 gttaaaatag gaataagaat aaatcctgat tttactatgg atgatgatag tgctaaatca
     5581 agcaaatttg gtatagatat agaagatatg agcagtgttt ttaagatgtt aagtatgtgt
     5641 gattatgtat ctgtagttgg gattcatata catattaaat ctcaggtatt ggattttgaa
     5701 aagttaggac attattatga aaaatgtttt tttatagctt taaatttaaa taaaagtaag
     5761 tatattaata ttgaatttat aaattttggt agtggacttg gagtagctta tgataatctt
     5821 agagaaaatc cacttaatct taaagagctt tcaaaattaa ctcaaaagat tgcttttact
     5881 aattcaaaca cactaaaatc aagacttctt attgaatcag gacgtttcat agtttgtaaa
     5941 tcaggtaatt attatgttaa aattgtagat attaaagtat caagagggaa aaaatatctt
     6001 attgtaaaaa atggacttaa tggatttatg agacctatta tttctaattt aattgaaaat
     6061 attacttgtg gagtagaatt agaaggtcaa gagccattat atacatcaaa tcatgccttt
     6121 tctctttcag tattaaataa tttaaaagaa gaagagtatg taaatgttgt tgggaatctc
     6181 tgtacatcat tagatgtaat tgcaaataat gttaaattga aaaaagcaaa cataggagat
     6241 ttgatttcaa ttagtaatgc tggaagttac tcctattctt tatcaccgct attattttca
     6301 agttatgaat ttcctaaaca atattataaa agaaaagatg gatatattga gtaaacaata
     6361 gcataaatta ataaacttag tattattgaa aaaatttaaa atgcaggggg tgtaattcta
     6421 tatggaattg tggaaaagaa atttatttgt atgttggata ggtatgtttt ttagttctat
     6481 agggatgagc caaatagcac ctatacttcc attatatatt aaacagttgg gggtaactga
     6541 tgttagttta atacaacagt attcaggtat aatttttgga tgtacctttg tagtagcagc
     6601 attcttttcg ccaatttgag ggaaagctgc tgataaatat ggaagaaaat caatgctttt
     6661 acgtgcaagt attgggatgg gaatagttat atttaccatg gcgtttgttc aaaatgtcta
     6721 tcagttatta ggattaagaa tacttcaagg agtttttacg ggttatgcta ctgcatgtac
     6781 tactcttatt gcaacacaga cagataaaaa tcattcagga tgggctttgg gtactcttgc
     6841 aactgcatca actactggct cattaattgg tccaacagta ggaggatata tagaatcaat
     6901 cttaggtctt aaaagtacat ttattataac aggaggattg ttgtttgtat catttatcat
     6961 ctctatttta tttgtagtag ataattttaa acctaaagaa ataaaagaat caatttctat
     7021 acctaaagaa caattaaata tattgccaaa taaattttta attgcatcta tttttgtaac
     7081 cacattcatt actcaactag ctttatactc tatagaacca attgtaacca tatatatatc
     7141 acaacttaca aattttgcat caaatgtagc tttaatagct ggattgacat tttctgcatc
     7201 aggattagca aatttattag cagctcaaaa acttggaaaa atgtcagata aaataggtcc
     7261 tcaaaaagta ttgttaattt cacttttatg ggctgggatt atatttatac cacaagcttt
     7321 tgtaaaaact gcatggcaac tgatgttgtt gagattttta ttagggttgt cagttgcagg
     7381 gcttaatcct tctgttaaca gcttgcttaa aaaaattgca ccagaagaat atgtgggaaa
     7441 aatatttgga tataatgcat ctgcccagta tataggctgt tcatcaggag catttttagg
     7501 gggacaaata tcagcacatt taggtattag aacagtattt ttttcaacaa gtttattgtt
     7561 gtttatgaat gcactttgga tgtataaatt tactggattg tttattaaag ataagactaa
     7621 ataatcatac tatttacatt tctaatttta tgatgtaaaa taaatatttt ttatgtaatt
     7681 tatcatagaa ttatataaaa aataacccta tggtaaaaat tggaaaatat tattatcgac
     7741 aaaatctttg acttaaaaac tatattgcga tagtattaat ttaataaaag tttgagaggg
     7801 gcgtaaggtt atgacaattt caaaaatacc acaagcagaa ttaaaagtta tgaagtttat
     7861 ttgggaaaaa aatgatacag taacatcaaa agaaactatt gaagctatgg aacgaaaata
     7921 tggttggaaa caaacaacta cattgacact tttatcaaga cttgttaaaa aacatttttt
     7981 aagtgcagaa aaaatagata gatacactca ttatacaata gttgttggac ataaggagta
     8041 tttaagtgtt gagacaaaag attttttaag caatatacac gacaattctt tacagagttt
     8101 aatttcagca ctacatgatg atgaagttgt tgataaagat atgttggatt tatttgaaag
     8161 tcattttaaa aatttagaag aagagtaatt atatcaggat agaactctaa gaatttaatt
     8221 aatctatggc tgaaaaccat aaatggtatt atttaatcag ctactcacta ttcataaagt
     8281 aatattaaat aggttctcta attagtaaaa tgcctaaaat aattggaata gggagtatca
     8341 agttaaatga taccacttat ttaaataaga aatgattttt aaaatttaaa ataattttat
     8401 aacttgtcaa tattcattaa tctaaagtat tatttctatt taaccatatc aaaatatatt
     8461 ttac
//
LOCUS       DAEORZ010000161         8446 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEORZ010000161 DAEORZ010000000
VERSION     DAEORZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.164"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..2075
                     /locus_tag="KRH39_003277"
     CDS             <1..2075
                     /locus_tag="KRH39_003277"
                     /inference="COORDINATES: protein motif:HMM:NF012632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513156.1"
                     /translation="NVKEIICGMYHTFILKEDGSIWSTGSNSYGELGLGDIAQRNIFT
                     KVDIDNVKKIACGQAYTFIIKNDGTVYSCGNNGSGQLGLGDTTNRNVFTKVNIDNVKE
                     VICASNHTFILKEDGSVWSTGNNGYSQLCLGDNTQRTTFTKVDIDNIKEIACSDSVTF
                     VIKNDGAIFSVGYNGYGQLGLGVSDGSVNEITKIEGHYLYRFDTLNNYTLNNNFNLIN
                     NKYIIPSRSDINYRNVLGSVYKEKYDIDNDRIELQENIKEFWMSKTHSLIVNTSGELF
                     GCGDNTYGQLLNPRTTALLTSFTKLDFADIRQASCGVGFSYFLKNDGTLYSAGLNSEY
                     QLGLGHNNEVNELQRVNISNVKKVMCDDKFTLVLLNDNSVWGQGLNRFGNFGLGQASA
                     NTIIKTFTKLDVDVEDVEIGGDYISFRKLDKSLWICGKIRDCYNMNSNNPYIFNKVQI
                     PAEFNNDLLWLDAGRDETLFMNYQAPTTKPSIEISEKTISGIKLKVNDSNDEIDKIEM
                     YLNNELIHSKNEFINNFVLFTIPLEKLNLGINNIYFKANTSFGDNMYLGATINKENNS
                     INISEGANLFIKNKKYNINSLTNSADGKLVITLDRALEDNISVGDIIYQLINQLKVQV
                     KTNNTGMHKDMNLLEMKKVDTGYQEVYELEEKGMTQVEPKIIVEKGDNWTAIKRPSMI
                     FSIDEETL"
     gene            2087..2410
                     /locus_tag="KRH39_003278"
     CDS             2087..2410
                     /locus_tag="KRH39_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513157.1"
                     /translation="MLNINEEKIEKLKQKKALNNLNTSQETQDNDITDLMLATAEICE
                     MVLSSQQTSTMSLKNIKLNEGGSSMAAIYVGLIERGLKTIEQVPIKYREEVRKMCEAL
                     EISLS"
     gene            2489..4441
                     /locus_tag="KRH39_003279"
     CDS             2489..4441
                     /locus_tag="KRH39_003279"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513158.1"
                     /translation="MLNEEVVKKLKNVPSNTNSEIGKVNTNIETAKTELNTKIGNTIL
                     LNTTDKTNLVNSVNELVDKQNDIENLEEIDNIKCNTDTGEYTIENSKKGYLTNFHIEG
                     KTLVNLANPNNITITEGLRYLNPFRYIEKGKQYTFINLSNKEIYYFHNNVIKFKMNPN
                     SKTLYTIDIDITDANCLCVAHSIKGWGETDADKQEFTKSVMILEGDYTSENISYFERL
                     QSVGQGDNTELLTFKNSENNLFDKNGNFRDNYILQYVNGEELISETSNHKYTLDYIEI
                     EADTGYTFYNCSRNICWYDINKSFIPASLNERIIGDKDIFYIARSPKNAKYLRVTIIK
                     DLHDNGNKTIITKGNKYDKKTILYTLRSLPNGVKDEIVYKNNKYYLIKRCEEHTYNDI
                     GNLNLSHVYDNTLQFMGTLTPRAVVDSLDTAYVLCNNLNGKSRNDFNNNDTEGCSTTG
                     SGDIAFKILKSKLTTQDGNGFSEWIKNNLVTIIYQLAKPQEIELTSLNLEQYNNQTKF
                     ICNTGIVIPDVSFESTQNLGSHIEVIRNNIKNYNVRTDFPFSINFLNGWQPYLGYASS
                     VGNYCTNNNFVTINATINGGVTTAGTIIGKIPSKYAPRKGIIVIFRTTDGKYYNGIIR
                     TNGEIEIYYNDITYRSWLYLYVNYLI"
     gene            4456..4776
                     /locus_tag="KRH39_003280"
     CDS             4456..4776
                     /locus_tag="KRH39_003280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513159.1"
                     /translation="MDIEKKIEILIEYGFENDLNNIENYFIAEGKKIFTPVIKNGELI
                     KTGEQVYNEWIELQNNPPKPSIEEINADKITILIENQKQQDSLLVDNAYRIAMLELNT
                     NNVL"
     gene            4802..4972
                     /locus_tag="KRH39_003281"
     CDS             4802..4972
                     /locus_tag="KRH39_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513160.1"
                     /translation="MYNILKRMIEQKNFETKEELQTKLDVFYAMNRIKEDEYTELTNL
                     LNKEDTLVEPKI"
     gene            5047..5862
                     /locus_tag="KRH39_003282"
     CDS             5047..5862
                     /locus_tag="KRH39_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8513161.1"
                     /translation="MNIKTLTAHAGHNPDGKLGSGAVGNIKESTEARNVLKELIPLAQ
                     KECKVYDTTCNNGTSQSNILNKIIDKCNSYNTDLNVSIHFNSGGGRGVEVLVYNLNDK
                     ETVEIASRICKKITETYHAKGDKDFKNRGVKEKKTLAFLRRTKAKSILVECCFVDTSD
                     TKKYNAKDMAIDIYEGIFNKSIAGKPQDNKVKYAIVYEGDVDKVIAQLMAMNYKANEV
                     SVCELKNYVPGHCENLYVIGGASSKIKTNERFTKLQGDDRWATLHKVLDFIGK"
     gene            5874..6491
                     /locus_tag="KRH39_003283"
     CDS             5874..6491
                     /locus_tag="KRH39_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG8513162.1"
                     /translation="MNILDKSTATLEQVFSYLDTLKNNSNPPHFLCRAIVPIIYKEAE
                     KKGVNPVIAIAQAFVETGYFNFGRVLNPSYCNVCGLKGNKGGGDLDPTAHTRFNCWED
                     GVSAFIDHLALYAGAKGYPKYNELVGKVEYKVNGTTLDPRHFSYLHGEAKTVESLSGK
                     WCPDTNYGQNIINICNKISSMKVENNDIKLEQIKTKVKELNEILG"
     gene            6503..6754
                     /locus_tag="KRH39_003284"
     CDS             6503..6754
                     /locus_tag="KRH39_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG8513163.1"
                     /translation="MDIMQFIPENLIMLIGGLYILGTFIKESNIKNKYIPFILLVIAM
                     ISSCLFMKELSIEAVFEGILCWGASIGINQIQVQNRKDK"
     gene            6755..7066
                     /locus_tag="KRH39_003285"
     CDS             6755..7066
                     /locus_tag="KRH39_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513164.1"
                     /translation="MLSKEMVELLSQYGYTAILLMVLMLWLGKYLEKNRQLEQDDRKK
                     ERAYFSREIKEQRTLFGNTIDKFDDKLDKFAEALNTNNSRLERVETDITKIKDKLETR
                     D"
     gene            7101..8036
                     /locus_tag="KRH39_003286"
     CDS             7101..8036
                     /locus_tag="KRH39_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG8513165.1"
                     /translation="MAKQRSKRISINGEPNPENIKLWKQYKRAKQIAGKSEKTIYNYE
                     CDIMQFFKFLNIECFDALLTDINEEEIEAYIGYCMEQGNNEKRIRRRISSISSLLSFL
                     KKKRKITDNWCEMIERPGTGEEVQKRTFLTEEQLSKLKEKLSEQDNLQLEVYINLGLA
                     TMARSNEISQFRWDNIDLDNRWIHGITAKGGVSRDFRFSEEVQHLLIEWKDYLSQNNI
                     DYPYIFFTRYGGKYNQVNSNVLSSWVKKAFRLIGIEGGYNHDLRHSMSNLLKDRGVPI
                     TTVSKLLGHSGVDVTINHYTIENKNKLAEEYDKFM"
BASE COUNT         3575 a          863 c         1450 g         2558 t
ORIGIN      
        1 ataatgtaaa agaaattata tgtggtatgt atcacacatt tatattaaaa gaagatggga
       61 gtatttggag tacaggaagt aatagttatg gagagttagg cttaggtgat attgcacaaa
      121 gaaatatatt tacaaaggtt gatattgata atgttaaaaa gattgcttgt ggacaggcat
      181 acacattcat aattaaaaat gatggtacag tatattcatg tgggaataat ggtagtggac
      241 aactaggttt aggtgacact actaatagaa atgtatttac taaagttaat atagataatg
      301 taaaagaagt catatgtgct agtaatcata catttatatt aaaagaagat gggagtgttt
      361 ggagtacagg aaataatggt tatagtcaac tttgtttagg tgataataca cagagaacta
      421 catttacaaa agttgatata gataatataa aagaaattgc ttgtagcgat tctgttactt
      481 ttgtaattaa aaatgatggt gctattttca gtgtaggata taatggttat gggcaacttg
      541 gtttaggagt aagtgatggt tctgtaaatg aaattactaa aatagaagga cattaccttt
      601 atagatttga cactttgaat aattatacat taaacaataa ttttaatctg ataaataaca
      661 aatatataat accatcacga agtgatataa attatagaaa cgttctaggc tctgtatata
      721 aagaaaaata tgatatagat aatgacagaa tagaattaca agaaaacata aaagaatttt
      781 ggatgtcaaa gactcattca ttgattgtaa atacttctgg agaattattt ggatgtggtg
      841 acaatacata tggtcagtta ttaaacccaa gaacaacagc tctattgaca agctttacta
      901 aactagattt tgcagatata agacaagctt catgtggcgt tggattctca tattttctga
      961 aaaatgatgg tactctgtat tcagcaggat taaattcaga ataccaacta gggctaggtc
     1021 acaacaacga ggtaaatgaa ttacaaagag taaatatatc taatgtaaag aaagtaatgt
     1081 gtgatgataa gtttacttta gtattattaa atgataactc tgtatggggg caaggactta
     1141 atagatttgg taattttgga ttaggccaag cttctgcaaa tactataata aaaacattta
     1201 ctaaactaga tgtagatgtg gaagatgtag agataggtgg agattatatc tcttttagaa
     1261 aattagataa atcactttgg atttgcggta aaataagaga ttgttacaat atgaactcta
     1321 ataatccata tatatttaat aaagttcaaa taccagcaga atttaataat gatttacttt
     1381 ggttagacgc tggaagagat gaaactttat ttatgaatta ccaagcacct acaacaaaac
     1441 catcaataga aatatcagag aaaactatat ctggtataaa attaaaagtt aatgattcaa
     1501 atgatgaaat tgataagatt gagatgtatt taaacaatga gttaatacat tcaaaaaatg
     1561 aatttatcaa taattttgtg ctgttcacta ttccattaga aaaactaaat cttggtataa
     1621 ataatatata ttttaaagct aatacttcat ttggagacaa tatgtatcta ggggcaacaa
     1681 ttaataaaga gaataattct ataaatattt cagaaggagc taatctattt attaagaata
     1741 aaaagtataa cataaattct ttaacaaata gtgcagatgg taaacttgta ataacattag
     1801 atagagcatt agaagataat ataagcgtag gagatataat ttatcaactt ataaatcaac
     1861 taaaagttca agttaaaaca aataacacag gtatgcacaa agatatgaat ttattagaaa
     1921 tgaaaaaagt tgacacaggg tatcaagagg tttatgagtt agaagaaaaa ggaatgacac
     1981 aagtagaacc taaaattata gttgaaaaag gcgataattg gacagcgata aaaagaccat
     2041 ctatgatatt cagtatagat gaggaaacac tttaagaggt gattaaatgc taaatataaa
     2101 tgaagaaaaa attgaaaagc tcaaacaaaa aaaggcttta aataatttaa atacaagtca
     2161 agaaacgcaa gataatgaca taacagattt aatgttagca actgctgaaa tatgtgaaat
     2221 ggtattaagt agtcaacaga catctactat gtcattaaaa aatataaaat taaatgaagg
     2281 aggaagtagc atggcagcaa tttatgtagg attgatagaa agaggtttga aaacaattga
     2341 acaagtacct ataaaatacc gagaagaagt aagaaaaatg tgtgaagcac tagagatttc
     2401 attatcatag aactataaga gttctttttt tatgttcaaa atggagagtt actaatgtag
     2461 ctctctaaat aaagaagaag gtgataaatt gttaaatgaa gaggtagtaa aaaaattaaa
     2521 gaatgtacct agtaacacta atagtgaaat tggaaaagtt aatacaaata tagaaactgc
     2581 taaaacagag ttgaatacta aaataggaaa tacaattctg cttaatacga cagataaaac
     2641 aaatcttgta aattcagtga atgaattagt agataagcaa aacgatatag aaaatctcga
     2701 ggaaattgac aatataaaat gtaatactga tacaggagaa tatactatag aaaatagtaa
     2761 aaaaggttat ctaactaatt tccatataga aggaaagaca ctagttaatt tggctaatcc
     2821 aaataacata actataacag aaggtttacg atatttaaat ccatttagat atattgaaaa
     2881 aggaaaacaa tacactttta ttaatttaag taataaagaa atatattatt tccataataa
     2941 tgtaatcaaa tttaaaatga atccaaatag taaaacttta tacactatag acatagatat
     3001 aactgatgct aattgtcttt gtgtcgctca tagtataaaa ggatggggag agactgatgc
     3061 agataaacaa gagtttacta aatctgtaat gattttagaa ggtgattata ctagtgaaaa
     3121 tatttcttac tttgaaagat tacaaagtgt tggtcaagga gataatacag agcttctaac
     3181 atttaaaaat agcgaaaata atttatttga taaaaatgga aattttagag ataattatat
     3241 tctgcaatat gtgaatggcg aggaacttat ttccgaaact agtaatcata aatacacatt
     3301 agactatata gaaatagaag cagacacagg gtatactttt tataattgta gtagaaatat
     3361 ttgttggtat gatataaata aaagtttcat accagcatca ttaaacgaaa gaataatagg
     3421 agataaagat attttttata ttgctagaag ccctaaaaat gcaaagtatc taagagttac
     3481 tataataaaa gatttacatg ataatgggaa taaaacaatt ataactaaag gaaataaata
     3541 tgataaaaag acaattttat atacattaag aagtttacca aatggagtaa aagatgaaat
     3601 agtttataaa aataataaat attacttaat aaaaagatgt gaggaacata cttataatga
     3661 tattggaaat cttaatttat cacatgtata tgataatact ctgcaattta tgggaacatt
     3721 aactcctcga gctgttgtag atagtttaga cacagcatat gttttatgta ataatttaaa
     3781 tggaaaaagt agaaatgatt ttaacaataa tgacacagag ggatgctcaa ctactggtag
     3841 tggtgatata gcatttaaaa tattaaaaag taaacttact acacaagatg gaaatgggtt
     3901 tagtgaatgg ataaaaaata atctagtaac tatcatctat caacttgcaa aaccacaaga
     3961 aattgaatta acatcactaa atttagaaca atataataat caaactaaat ttatttgcaa
     4021 cacaggtatt gtgataccag atgttagttt tgaaagtaca caaaatttag gaagtcatat
     4081 agaagttatc agaaataata taaaaaatta caatgttaga actgattttc cattttcaat
     4141 aaactttctt aatgggtggc agccatatct tggatatgct agttctgttg gaaactattg
     4201 cacaaataat aactttgtaa caattaatgc aactattaat ggaggtgtta caacggcagg
     4261 tactatcatt ggaaagatac cttcaaaata tgcacctcgt aaaggcataa tagtaatatt
     4321 tcgaacaaca gatggaaagt attataacgg aatcatccgt acaaatggag aaattgagat
     4381 atattataat gatataactt atcgtagttg gttgtattta tatgtaaact atttaattta
     4441 gaaaggaaaa ataagatgga tatagaaaag aaaatagaaa ttttaataga atatggattt
     4501 gagaatgatt taaataatat agagaattac tttattgcag agggtaaaaa aatatttact
     4561 ccagtgataa aaaacggaga gctaattaaa acaggagaac aggtttataa tgagtggata
     4621 gaattacaaa ataatccacc taaaccaagc atagaggaaa taaatgcgga taaaattaca
     4681 attttaatag aaaatcaaaa acaacaagac agtttattag tagataatgc ttatagaatt
     4741 gctatgttag aacttaacac aaataacgtg ttataaaatt ataaaaaatg gagggaagaa
     4801 tatgtataat attttaaaaa gaatgattga acaaaagaat tttgaaacta aagaggaatt
     4861 acaaactaaa cttgatgtat tttacgctat gaataggata aaggaagatg aatacacaga
     4921 gttaacaaat ctattaaata aagaagatac actagtagaa ccaaaaatat aaaaggttct
     4981 ttttttatgt caattttaga gaattgatta atttcaattc tcttttaatt aaaaggagtg
     5041 gtaattttga atataaaaac attgactgcc catgcaggtc ataatccaga tggtaaatta
     5101 ggttctggag cagtaggaaa tataaaagaa tctacagaag caagaaatgt actaaaagag
     5161 ttaatacctt tagctcaaaa agaatgtaaa gtttatgata ctacatgcaa taatggaact
     5221 tctcaaagta atatattgaa taagattata gataaatgta attcatataa cacagattta
     5281 aatgtaagta tccattttaa cagtggtggt ggtcgaggag tagaagtttt agtttataat
     5341 ttaaatgaca aagaaactgt tgaaatagca tcaagaatat gtaaaaaaat aactgaaact
     5401 tatcatgcaa aaggtgataa agattttaaa aatcgtggag ttaaagaaaa gaaaactcta
     5461 gcatttttaa gaagaacgaa ggcaaagtca attttagtcg agtgctgctt tgtagacaca
     5521 tctgacacta aaaaatataa tgctaaagat atggccatag acatttacga aggaatattt
     5581 aataagtcta tagctggtaa accacaagat aacaaagtga aatatgcaat agtttatgag
     5641 ggtgatgtag ataaagttat agctcaatta atggccatga attataaggc taatgaagtc
     5701 tctgtgtgtg agttgaaaaa ttatgttcct ggacattgtg aaaacctata tgtgattggt
     5761 ggagcatcca gtaaaattaa aactaacgag agattcacta agttacaagg tgatgataga
     5821 tgggctacac ttcataaagt gttagatttt ataggcaagt aggaggtaat gaattgaata
     5881 tattagataa atcaacagct acattagaac aagtcttttc gtatttagac acattaaaaa
     5941 ataactctaa tccaccacat tttttatgca gagctatagt accaattata tataaagaag
     6001 cagagaaaaa aggtgtaaat ccagttattg caattgctca agcatttgta gaaactggat
     6061 attttaattt tggtagagtt ttaaatcctt cttattgcaa tgtgtgtggt cttaaaggaa
     6121 ataaaggtgg aggagattta gacccaacag ctcatacaag atttaattgt tgggaggatg
     6181 gagtatcagc atttattgac catctagcat tatatgctgg agctaaggga tatccaaaat
     6241 acaatgaatt agttggtaaa gttgaatata aagtcaatgg aactacatta gaccctagac
     6301 atttttcata tctacatgga gaggctaaaa cagttgaaag cttatcaggt aaatggtgtc
     6361 cagatacaaa ctatggtcaa aacataataa atatatgcaa taagataagt tcaatgaagg
     6421 tagaaaacaa tgatataaaa ctagagcaaa taaaaactaa agtaaaagaa ttaaatgaga
     6481 ttcttggata ggaggaaatc acatggatat aatgcaattt atacctgaaa atttaataat
     6541 gttaataggt gggctttaca tattaggaac atttataaaa gaatctaata ttaaaaataa
     6601 atacatacct tttatattgt tagttatagc aatgataagt agttgtttgt ttatgaaaga
     6661 gctgtcaata gaagctgtct ttgaaggtat tttatgttgg ggtgcatcta taggaataaa
     6721 tcaaatacaa gtgcaaaaca gaaaggataa gtagatgtta tcaaaagaaa tggttgaatt
     6781 actaagtcaa tatggatata cagctatact tttaatggta ctgatgttat ggcttggaaa
     6841 atatttagag aaaaatagac aactagaaca agatgataga aaaaaggaaa gagcttattt
     6901 ttcaagagaa attaaagagc agagaacttt atttggtaat acaatagata agtttgatga
     6961 taaattagat aaatttgctg aagcattaaa tactaataac agcagacttg aaagagttga
     7021 aacagacata acaaaaatta aagataaatt agagactaga gattaatttc tctagtcttt
     7081 tttataagga agtgaagtat ttggcaaaac aacgtagtaa aagaataagt ataaatggag
     7141 aaccaaatcc agagaatatt aaattatgga agcaatacaa aagagcaaaa caaatagctg
     7201 ggaaaagtga aaaaacaata tataattatg aatgtgatat aatgcaattt tttaagtttt
     7261 taaatataga atgttttgat gcactattaa cagatataaa tgaagaagaa atagaagctt
     7321 atataggtta ttgtatggaa caggggaata atgaaaaaag aattagacgt agaataagtt
     7381 caatttcatc cttactatca tttttaaaga agaagaggaa aatcactgac aattggtgcg
     7441 aaatgataga aagaccagga acaggcgaag aagttcagaa aagaaccttt ttaactgaag
     7501 aacagttaag caaattaaaa gaaaagcttt cagagcaaga caacttacag ttagaagttt
     7561 atataaattt aggattggct acaatggcta gaagtaatga gataagtcaa tttagatggg
     7621 acaatataga tttagataat agatggattc atggtattac agctaaaggt ggagtttcaa
     7681 gagattttag attttcagaa gaagtccagc atctattaat agaatggaag gattatttat
     7741 ctcaaaataa tatagattat ccatatatat tctttacaag atatggaggc aaatacaatc
     7801 aagtcaattc aaatgtctta agttcatggg ttaaaaaagc tttcaggtta attggtatag
     7861 aaggtggata taatcatgac cttagacata gtatgtctaa tcttttaaaa gacagaggag
     7921 ttccaataac tacagtttca aaattgcttg gccacagtgg agttgatgta actataaatc
     7981 actacacaat agaaaataaa aataaattag ctgaggaata tgataagttt atgtaaattg
     8041 aaaggaagtg atataatggc atgtaaaggt cgtaaaaaag gtaagaaaaa gagatagata
     8101 ataagccctc tagggattac tcttaattga gttttcttta gagggcttta tttttttatc
     8161 tatgtttata taaatttata attatgtata atactaaacc acctagaata cttttcaaaa
     8221 aaaaatgatt ttatttgaaa ttttttaaat aaaacatcag aaacataaaa tacgacacat
     8281 gctataaaaa aatataaaaa ttcattatta attcttgcca atctaatatg tatgatagta
     8341 aataatatta taataattaa aaacgtatct ataaaatttt tgcgttttca tttaatatat
     8401 taaacttatt ttaattaggt atctatttca agtctattct gatagt
//
LOCUS       DAEORZ010000162         8379 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEORZ010000162 DAEORZ010000000
VERSION     DAEORZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.165"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            66..296
                     /locus_tag="KRH39_003287"
     CDS             66..296
                     /locus_tag="KRH39_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBG8513166.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            309..458
                     /locus_tag="KRH39_003288"
     CDS             309..458
                     /locus_tag="KRH39_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBG8513167.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            715..1152
                     /locus_tag="KRH39_003289"
     CDS             715..1152
                     /locus_tag="KRH39_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG8513168.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            1416..2309
                     /locus_tag="KRH39_003290"
     CDS             1416..2309
                     /locus_tag="KRH39_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG8513169.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIGIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            2487..3311
                     /locus_tag="KRH39_003291"
     CDS             2487..3311
                     /locus_tag="KRH39_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8513170.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            3410..4018
                     /locus_tag="KRH39_003292"
     CDS             3410..4018
                     /locus_tag="KRH39_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8513171.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            4122..4298
                     /locus_tag="KRH39_003293"
     CDS             4122..4298
                     /locus_tag="KRH39_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513172.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            5494..6246
                     /locus_tag="KRH39_003294"
     CDS             5494..6246
                     /locus_tag="KRH39_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513173.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            6236..8347
                     /locus_tag="KRH39_003295"
     CDS             6236..8347
                     /locus_tag="KRH39_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513174.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         3175 a         1075 c         1112 g         3017 t
ORIGIN      
        1 aattaaaata ccaatattaa caagcaaaca attaaaatat aaattattat atcggaggta
       61 attatatgga aattaagaaa aaattagtca tgagctttaa aaatgtaaag gataaacaag
      121 tttctttttc tatagaaaat ccaaaggatg atttaactga agaaaatata aaatctgtca
      181 tggatttgat agtatctaaa aatatttttt cggttggagg atttgaccta gcttctgttg
      241 ttgaagccaa ggttgtggaa acaaatacca ctccttatga cttagttata gaataggggg
      301 ctaattttat ggatgatagt ttacaactgt taataaccaa tgttggtttt cctatagctc
      361 tatccatgta cttattggtt agaattgagg gtaaattaaa tactttatca aacagtattg
      421 atacattatc caaaaatatt ttgatattta agaagtaatt tgacaatata tgtaagtcat
      481 aaaataggtc tatttgtaaa ataattagac atattctatg tgaaatacta ggatatttgg
      541 ataatttatc caaatatcca tagtattttt ttgaatttta ccacctcaaa tttgaatttc
      601 actgttttat ttttgaattt cactatgctt aatttgaaat ttagaacgca tctagttgga
      661 ctcaataaga tataatgcta ttctaatgac agtaataaaa tgaggtgatg attaatgaaa
      721 atacatgtcg aacaaggtat taattgtgat gatattgaaa taaccataaa atgttctgct
      781 ctagataata acattgaaaa aataatttca ttattgagta caccgttact tgatataaaa
      841 ggtaaaaaat caggagagat ttataaactc aatgtctgtg atattttata ctttgagaca
      901 gttggaaata aaacatttgc ttattatgaa gatgatgtat ttgaaattga tttaaaaata
      961 tataaattat caaaattatt agctaataca tccttctcac gaatatctaa atccatgatt
     1021 ttaaatatgg actacataaa aagtataaaa gtaatatcac ataatcgcat gcaagctact
     1081 ttaaaaaaca acgaaaaagt tattataaat cgtcgttata taaaaaatat taaagaaaag
     1141 ctaaacattt aatatttaat aaaaatctaa agtaatttat taatatcact aattgcaaat
     1201 ttattgacct atttaataat taataataat ataatatatt caatattaaa tactaattaa
     1261 ttatatttag tgtaaaaata attaatcaaa atataaaaaa taggatatta ttataggcaa
     1321 taactaacat caagcgataa cttaatacat ataaaaataa ataaacaatt aataaaaatt
     1381 aaaacttcat tataaaacgg aaggatagtg aaaaaatgca actatataac acccccttta
     1441 atacaaacaa acacttaatc tatttcttta catttacgct agtattgaac tggatattca
     1501 gtcttacacc tgcgttgctc ggaattacaa atacaatact tggagaaatc attttttatc
     1561 ttgcatatac atcaccagct attgtaggtc tattttttat ttctacaatc tatccacaaa
     1621 gtgcaaaaca agattttatt tatagatacc taagctttaa agaaattgga ataaaatgga
     1681 tactattaac aatattattt tttgctttaa cttttattac ttcgttaata atcggaaatt
     1741 actttaatgt aaaaacttct gatatagatt ggatacacat agcagtgttt agaccttcta
     1801 aaatagctta tatgctatta gcaagtttta tattgagtat acttagtcaa gaagctggat
     1861 ggagaggata tgccattgac aaattattag ttagatttgg atttataggc tcttcaatta
     1921 ttttagggct tgtttgtgga atctggtatc tagggtcgta ttttacacct aatcaaatac
     1981 catataattt ggcacaatac tccctatttg atgctttttt atttatacca aacattgtac
     2041 ttttaaactt tataattaat tttgtatata tcaatacaag tagaagcatt ttatcagctg
     2101 ggttagttca tatgatgtac tattttttca acattcaatt acttctacac taccctatta
     2161 aattatgtgt aatagctcaa tatgtacaaa taatatttgg tttaatattt cttgtatata
     2221 tgatttcttc taataaattt aaacagaagg taaattctga aattgaacag ataaagtcgg
     2281 atgattttga atttgagtta gactggtaat tttaatatga attataaatt aagataatct
     2341 atgatttgat acgattacta cttaaatata atttactctc tataatttat tgcaacatct
     2401 ggaaaataat tgtatttcca caaacgcttg aagtatataa aaacaaaata tatactgatt
     2461 ataagtgaaa aggaggtcat attttcatgt ttaaaatagg agaggtttca aaactaacac
     2521 aaatttctat tcgtatgttg cgttattatg atgaacttgg tattttaaaa cctgcagaaa
     2581 tagacaaata tacaggtcat agattatact cagttgaaca gatttcaata ctacaaagaa
     2641 ttgtattatt gagagattca aaatttagcg ttgcagaaat agcaaatatc gtacataatt
     2701 ggaatgatga atttgtaatt aaggaattga atagaaaaaa gaatgaaata caaaaggaaa
     2761 taaagcagga acaacaaaga ataaataaaa ttgataaatt tatagaagct ataaactgtg
     2821 ataaagatga aattcattac aatgttgtct ttaaaaagat tccaagttat aaaatcatat
     2881 cacttagaga gataattcca gactaccaat ctgaaggtat actctgggaa aagctatcta
     2941 aatttatcaa agaagaacat atagaagtat cacgtcaatc caacaataac atagcttttt
     3001 accatgatga agaggtcaaa gataatggtg tagatattga agttggaatg gttgtaaaga
     3061 agattggaaa gaacaaatct ggatttatat atcgtgagac agaagaaatt gatatgatgg
     3121 catgtactat ggtttatggt ccatatgaaa atatagctgg tgcatatgaa tcgttctgtt
     3181 attggttaga taaaaatagt gattatcaaa tatcaggtat aaatagacaa atcggacata
     3241 aaggtgaaca taatgaaata aatcctgaaa attatcttac agaaattcaa atccccctta
     3301 taaaagtcta atagattagt acttaaatac ttaagtacta atcacccatt gacatcccaa
     3361 aattttcatg atagtatcta aacgtgtatt aaacatgttt aattttttta tggagaaact
     3421 tttaagggga aaaattatga aagcagattc aacaaaggaa caattaatta atgccacaat
     3481 ccagttacta tcttcacata gagatgtttc aagcattacg gctagagaaa ttgtagctaa
     3541 agcaaatgtc aatttagcta tgattaatta ttattttaaa tctaaagagg agttaattaa
     3601 tatttctatt aaaaaatttt taaagcgtca tatggatgaa tataaggcta cacatgatac
     3661 taaaaattta gcacctaaac agcaattaag ggaaatacta attgcatttt ttgattttat
     3721 aggtgaacat agtcaattta taaagatttc tgtaccctat atattgatac aggaagaaat
     3781 tgtttatcca tttgagatac tacctttaat taaggctcac tatgaaaatc agaataacaa
     3841 aaaaactgaa gctgaataca gaactattgc gtatcaaata acttcattta ttcagttgct
     3901 ttttcttcgt tctaatgctt ttttcaagta tagtggtatt gatattctta atgttgaaca
     3961 gagaaatggt ttaatagatt ttcaattaga tttattattg gtagatatta caaaataata
     4021 ctatattaat tttttatgta cattttttta ttttatggta acgtttgaaa atattctcat
     4081 tgactaaaaa tacttgcatt aaataagggg gaggaatttt aatgtttttt acatttatag
     4141 gagtaatatc agcaatttta ttgttgtgta caattatatg tggtttatgg ataaaactta
     4201 ataatcgtaa taatgactct aattctcact ttactttttc aatactttca attgcttttt
     4261 cactgttaat tgtggtggcc tttttagtaa gaaattaatg tctttgttat gctggggttt
     4321 attattatag atttttatta ttgcatgatt tttactatat tatagtattt ttattatatt
     4381 acagggtctt atttattata gtgttttgtt gttacagcat tttattatat tacagagtcc
     4441 tatttattat aggattttta ttatattgta aaatcttatt tattatagga ttttgttata
     4501 ttacagactt ttattatatt acaatctctt atttattaca gtatttctat tatattgcaa
     4561 actcttattt attatggtat tttgttattg caacatttct attatattgt aaagtcttat
     4621 ttattacagt tctttattat actacagact tttattatta caacctctta tttattacag
     4681 ttctttgcta tactacagac ctttattatt acagcatttc tattacattg caaagtctta
     4741 tttattacag ttctttatta cactacagac ttttattatt acagcatttc tgttacattg
     4801 caaagtctta tttattacag ttctttatta tactacaaac ctttattatt acaacacttt
     4861 attattttaa cttctttact ataatatagt aatattttaa tatctcaaaa tcatatcaat
     4921 cacttttcag catagtctaa aatacttcca ctttctaaaa ttatctatca tacctatact
     4981 aataatactt tttaaccttt ttattataca tttcaattgt ttttaacaac ctatttattt
     5041 ccaaaactcc aatatatttt ctcaattaca ttgtgttaat aacccctaaa tcctttcaaa
     5101 ttatttacaa tacattccat taaataatct tactgctcta taattctttt tttcttttta
     5161 accctactat acattattta catcattttt ttcaatacaa cattcgttat tctataacct
     5221 ttttactttt actatatacc tcttttctaa ctacaccttc aatgttccac atcatattac
     5281 tttagcacaa caattaatac ctaaaaataa ccctctaaat cctccaataa tataatcaat
     5341 ttaactataa taataacctc tcacactatc caaacaatat attttcaaac attacaaaaa
     5401 aacatccaac tattacaata tagctcatat ccctcctccc ctttcaaatg ctatcattga
     5461 cttacaagat aaatgaaagt gaggatgata tatatgctta aagtaaacca attatgtaaa
     5521 tcatataaaa caggcagtaa aacatatcct gtattaaaaa atatttcttt tgaaatcaac
     5581 aaaggagaat ttgtcgcaat catgggttca tcaggaagtg gaaaaactac tcttttaaat
     5641 tgtatttcat gttttatccc aattgataat ggggaaataa ttcttggaaa taataatata
     5701 agaaatttaa aagaaaagga actttcagaa gttagaaacc aacaactcgg atttgtattc
     5761 caagatttca tgctatttga tggtcttagt gtatttgaaa atatatgtgt tccacaaatt
     5821 atcaaaggta gtcaagtctt acaaatggaa aaccgtgcaa ttaatctatg tgagctattt
     5881 ggtattgaac atatcagaaa aaaataccct gccgaaatat ctggtggaga aaaacagcgt
     5941 acagcagtag caagagcact catgaacaac ccagatttaa tattagcaga tgaaccaact
     6001 ggtaaccttg atagtaagtc ttgtcgtaca gtcattgaat catttttaaa agcaaagcaa
     6061 gaattagatg ctactatact catggttaca cacgatagct ttgcagcaag cttctgtgac
     6121 cgagttattg tgcttaaaga tggaataatt tataaagaag ttgtaaaatc aaattcaaga
     6181 cataaattcc tagatgaatt actagaagtt ttaaaagaaa tgggaggcgc tcaagatgac
     6241 aattaataaa ttatacagta aacttcgagc ctataattgg aaaaactata ctactttatt
     6301 cttttgtata atactttctg tggtattagt aacttcatat gctctaattt actttagccc
     6361 aacaatacaa aacatacttc caactggtgg agatagcaga aaacaggcta caatgatatt
     6421 ctcagttgct atattaggat gcgctgtctt tactacatac gcttcttccc tctttttaaa
     6481 atttaaaagc aaggaaattg gtattttcat ggctctagga acagataaga agaaattaaa
     6541 aaaacttcta ttttcagaga tattgttaat ttcaatagta tgctgttttt taggtcttat
     6601 attaagtatt cctgtatctt ttggtatatg gaaaatattt caattagtga ttattgatac
     6661 cagagaaatg acatatcatt ttggaactca aggattcata tatggattta tatttagtgt
     6721 atttgtgaca ttatgcatat ttatactttc tgtaaaattt attaatcgta caaatataat
     6781 tgatatatta aatgaccaga gaatatctga accaatacag gatgtaaagg actggtatgg
     6841 tatattaggt ataattttta ttatactagg tatttttcta ggatatctcg taccttcact
     6901 aactataaac aaattaaatt atagactacc atctgtatgg agctttactt atctactctc
     6961 tcttattgga atatatatga ttatgacata catagttgta catgctaaaa gaggaaaaca
     7021 tccagagaaa tactataaaa acatcatatc aactagcatg atgagattta tgggaagaca
     7081 aacagtaaaa aacatgtgtg ttatttcatt tttgattgca ggagcattat ttgctgtatt
     7141 ctatgttcct aatatggtta caggtatcta tgatactctt aaaaataacc ctatagatta
     7201 tacttttacc tacaatcaaa gagataaaca ggtatcaaaa aaagatattg aacaattagc
     7261 agataaacac aatataaaaa ttcaaaacta tatggaaatt ccttctatag aattaattgt
     7321 tgatggtgta gaatcaatat atcataaaga tggaactatc gaaaaggtat atatgaaaaa
     7381 aagctcctat gatatgttct ttaattccaa agatttttcc aagctaattg gcaaaccaat
     7441 aactataaaa aatggtgaat atcttgctgt tgtaaataaa ggaaatgaaa atcaagatta
     7501 taaatatacc aaatttatat caagcccagt cacagaaaaa gaaatcccct ttagatatgc
     7561 tggaaaaata atatttaatg gttctttttt tcaaaataga agtatgaacg cctatgtcct
     7621 taatgatgag gattataaat catttactaa agatttatcc ataaaaaatt acaataattc
     7681 tatactattt aatgtagata aagataccta tgcttttgcc aatgacttaa agaaagaaat
     7741 tataaaaaga acatctaagg aaatgggcat gttcagtggt tatgatgaat acgtaaaaaa
     7801 acgttatgaa tcaactggaa gaaaatattt tatggatgaa gaatatccaa gtggtgaagg
     7861 tcatttaaaa ctagacccaa acaacaatca gttgtatctt aattggaaat actatcctaa
     7921 ctttaaaatt ttacaaagcc aagatatgat aaaaaacatg gcagtcttct taatgttatt
     7981 catatatgtg gcaatcatat gttttacagc agtaggaatc attgcatata caaggggtgt
     8041 tactatagct ataaatcaca agcaggtctt tatggattta aatcgattag gagcaaacaa
     8101 tgattatata aagttctgta ttagaaatca attaaagaaa gtattttcat accctcttat
     8161 tgcaggaagt tttttaattt atctgtttaa ttttatgatt atgttaaata atgatggtag
     8221 aatcgtcaat agtgaggtca atgctctttt gataaactta ggttttatag ggatttgtgg
     8281 ggtttatatg taccttatat atctattaac ttataagaaa ttcaaaaaaa ttattggtat
     8341 tttataataa aaaaggttat tagattttta aagtaataa
//
LOCUS       DAEORZ010000163         8264 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEORZ010000163 DAEORZ010000000
VERSION     DAEORZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.166"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(16..1692)
                     /gene="bclA2"
                     /locus_tag="KRH39_003296"
     CDS             complement(16..1692)
                     /gene="bclA2"
                     /locus_tag="KRH39_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBG8513175.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGENGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2070..2609)
                     /gene="hpt"
                     /locus_tag="KRH39_003297"
     CDS             complement(2070..2609)
                     /gene="hpt"
                     /locus_tag="KRH39_003297"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG8513176.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2937..4244)
                     /locus_tag="KRH39_003298"
     CDS             complement(2937..4244)
                     /locus_tag="KRH39_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBG8513177.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4669..6444)
                     /locus_tag="KRH39_003299"
     CDS             complement(4669..6444)
                     /locus_tag="KRH39_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8513178.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6441..6578)
                     /locus_tag="KRH39_003300"
     CDS             complement(6441..6578)
                     /locus_tag="KRH39_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513179.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6656..7225)
                     /locus_tag="KRH39_003301"
     CDS             complement(6656..7225)
                     /locus_tag="KRH39_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG8513180.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7409..8044)
                     /locus_tag="KRH39_003302"
     CDS             complement(7409..8044)
                     /locus_tag="KRH39_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG8513181.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2354 a         1661 c          945 g         3304 t
ORIGIN      
        1 ttttcatcat ataaactatt gtattctata aactgataca tatccagctg cagaagtacc
       61 tgtcgtgcct gaaccaaatg taaaacttaa agtaacagct gctgctctag catcaactaa
      121 aaagcttaaa cttacactta catctgatga agtaagtgta tttactgctt ttgcaataaa
      181 gttattttga gctactccat taaagttagt agtaattgac ataaatcctg caagtgaaag
      241 tgtggcacgt ataccgaaag atacattgta tatccctcca actgcaagat ttatagtact
      301 acttgttgga aaagttatat tagttccatt tataaatgat gaattaaatg tcactaatgt
      361 attattcact aaacttgaag aggacactgt aaattgtgca ttgtttgctg ctaacactcc
      421 tgttgctcct gtcgctccgg ttggacctgt tgctccattt gctcctgttg ctcctgtcgc
      481 ccctgttgct cctgtcgctc catttgctcc ggttgggcct gttggtccta tcgctcctgc
      541 tactccattt gctcccgttg ctcctactgc tcctgttggg cctgttgctc catttgcccc
      601 tgttgctcct gtcgctcctg ttgctcctgc tactccattt tctcccgttg gacctgttgg
      661 acctattgga cctgctactc cattcgctcc cgttgctcct actgctcccg ttgggcctgt
      721 tgctccattt gcccctgttg ctcctgtcgc tcctgttgga cctactgctc catctgctcc
      781 cgttgggcct gttgctccta ctgctcccgt tggacctatt gccccagcta ctcctgttgc
      841 tcctgtcgct cctgttggac ctaccaaacc atttgctccg gttgctccgg ttgggcctgt
      901 tgggcctacc aaaccatttg ctccggttgc tccggttggg cctgttgggc ctgttggacc
      961 taccaaacca tctggacctg ttgctcctac tgctcctgtt ggacctacct ctccatctgc
     1021 tcctgttgct cctgttgctc ctattgggcc tgttgcccca gttgttcctg ttgctccagt
     1081 cgctcctata cttcctgttg gacctgttgc tcctgtattt cctgttgggc ctgtagcacc
     1141 tgttactcca tttgctccgg ttgctcctgt atttcctgtt ggtcctgtta ttccatttgc
     1201 tccggttgct cctgttgctc ctgttagtcc ggttggacct gttgctcctg ttgctcctgt
     1261 attccctgtt ggacctgtta ttccatttgc tccagtcgct cctgtatttc ctgttggtcc
     1321 tgtagaacct gttattccat ttgctccggt tgctcctttg tttcctgttg gtcctgttat
     1381 tccatttgct ccggttgctc ctgtattccc tgtcggacct gttattccat tcgctccagt
     1441 cgctcctgta tttcctgttg gtcctgtaga acctgttgtt ccatttggtc cagtcgctcc
     1501 catatttcct gttggacccg ttattccatt cgctccagtt gctcctgtat ttcctgttgg
     1561 tcctgttatt ccatttgctc cggtcgctcc agttgcacct ctaggccccg tcggtcctgt
     1621 tggaccagta gcacctgttg gccctgttgg acctacatct tgatataaac ttggacctga
     1681 aatatcactc atataattaa tcctcctttt ttaaagttag agtaatactc tataaatata
     1741 gagtattact ctatataatt atatgaatcg acaatttctt atgtgataat gtatctattt
     1801 gcttatatca acatatatgc tttgattctt aaatttcatt acttttttgt aaaatctttt
     1861 acaattatta gtactctttt acaattatta atacaattaa aaaactcatt tcccatcaat
     1921 tattcaaaat ttttttatta tgctataaaa aatagataat taaattgcat aaaattaatt
     1981 taatactact ttaattactt tttaaataaa aaaacctcaa cacgattaat aaaaatcata
     2041 ttaaggcatc cacaaaatta tataattgct tatctatctt catcagtaac attgaatata
     2101 taaggaacat ttctataata atcaccaaaa tcaaacccat atccaaccac aaacttatct
     2161 tctatttcaa atccacaata atctggcact aaatcgactt ttcttctgct tggtttatcc
     2221 aaaagaacac agcattttaa actagcagga tttttagctt ttagatgttt taacacaaaa
     2281 tccatagtaa gtgcagaatc agtaatatcg tctacaacca aaacatcata tcctgctata
     2341 tcagtagtca catctgaaac tactttaacc cttccagtac tttcttcatt gtttccataa
     2401 ctagatgtag tcataaaatt aactctaagt ggtaagttta tatttctaac taagtctgca
     2461 caaaatataa agctaccttt taatagagaa actaccataa gatttttatc tttataatct
     2521 ttttctataa ttgctcctaa ttcccttaat ttatctttaa ttttttcttc tgaatacaat
     2581 acttcccatt tttttgtttc tatatccata aaaagacctc caattgtaat aatccttctt
     2641 aattttataa gagattgctc actttttcaa tactcttatt caatattagt caatattatt
     2701 gagtttttat gttcattcaa caattattaa caatgtatct tatattttaa gtagttcata
     2761 tatccacact aaattacata ctatatattt tagacaaaaa aagaaaaaac ttgcactaac
     2821 aagttttttc tattatacat aaatttatac ataatcatat tttattagag ggtactaatt
     2881 aaatttattt atgctatttt tgttatacct cataatacaa aacatattat aaagtactag
     2941 tcaactttat tcatatcatt cattacattt aagattatct catctgttat agacatacct
     3001 ttgtcactta ctctacctaa gttttgtata ctttgctcaa cacttgaacc aactatacca
     3061 tttagtggtg gtacaaaaca atcttgctta gctattatag cactttgtat agctgcagat
     3121 gctgcagaag ataattttaa tgcacaacca gctttcgcac cgtcacatat cattccactt
     3181 aagtcagcta tcatattttc tatagctcct tctatttgct tttcagtacc attcataagc
     3241 caagagatac cagcagttgc tccagtagaa gcagcaacac cacatccaca tacagcagat
     3301 aatcttcctg tatagttctt aatataacca gttactagat gagatatagc taaagctttt
     3361 gctagtcttt catcagattg tgggaatttt ttattataag caacgattgg aagaattgct
     3421 gttaatccat gatttccact tccatttgaa ctcataacag gcatttttac ccctgccatt
     3481 cttgcatctg aagctccagc agttaacatc attgcataat ttataacatc attacctaaa
     3541 agaccttcct ctatagactt tttaattcca tacccgacac caataccagt tttttgtttt
     3601 aatccatatt cagccatctc ttcattcatt tttactccat ctagtaaaaa ttctatatct
     3661 ttaaaatcaa tttcttctac attttttatt aattcttgta tagttactgt atccattaaa
     3721 ctttcagctt tttctgccgc atcatttgaa gctgatactt ttggttcatt gtctaataat
     3781 acttctccat ctttttctaa gaacgtaaaa ttatcatgtt tagttcttat tctcacttta
     3841 gcaatatgat tttttccttt taacacaact tctataaata ctttctctct tgtatcagca
     3901 ggtgttattg atagtggagt attatccata taatcctcag ctattttaac ttcctctttt
     3961 gttagagttt ctaacacact taatccattt tcagaatgtc cacctactat acctagagct
     4021 gctgcaattt ttaatcctaa tctttcagta cctggtatac ctacgcacat accattttta
     4081 taaatactag gactaactaa catacgattt tcaactattt cttctcctaa taattccttt
     4141 gcctttgcac atgctagagc taaggctact ggttcagtac accctactgc tggtttaact
     4201 tctgccttta acatttccac taattctttt cttatatctc tcattatata taaacctccc
     4261 aattaagttt agatttagta ctcaaataat taattaaata atttcttatc tatatttata
     4321 gcaaaataca tgccatattt tttatatctt tttatatttt tagtcttata ttctataacc
     4381 attggtatga ctgcttctat ttatgtattt tttttattta catagagtat ttatattctt
     4441 taaaatggag aaacatatga caatttcgca aaaagacaaa aaaaagtctc aaaatgagat
     4501 tctatatcat tttgagacgg ctcaaatata ttatttttta acttataaaa aacatttttt
     4561 aattcataaa aaaagattct gcaaaacact tatagtataa gtttaaagct ttctttaaag
     4621 ttatgaattt agaaaaattc aaattttata tagcttttta ttatattact atgaaattat
     4681 attgtatttt tcaatttttc tatataatgt agctcttgat attccaagtg ctttagctgt
     4741 taattcttta tcttttttat aatctttgta cttatttaaa gcttttatta tctcaatctt
     4801 ctctagttca tcaagtggaa ttatcctatc gcttttaact tctaactctt ctacagaggt
     4861 attcttaatt ttttgaggaa ttacattcat tgtgattatt gaagaagaag acatattcac
     4921 actatattca ataatattct gcaactctct tatattccct ggccacttat attccatcat
     4981 agcatcaaca accctgctat ctatgtcaat aacattttta tctaattttt ttgaatactc
     5041 tttaaccata tgcttgatta taagtgggat atcttctttt ctctctctta aacttggtaa
     5101 ttttataggt ataacattta atctatagta taaatcttct ctaaaagtgc cattttgaac
     5161 cattttttct aaatctttat ttgtagcagc aataattctt acatctattg aaacatttga
     5221 tttacttcca attttattaa gttccttttc ttgtaatact ctcaataact ttgactgtaa
     5281 atgcaaactc atatctccta tttcatctaa aaatatactt cccttatgag ctacttcaaa
     5341 cattccaagc tttccacctt ttttagctcc agtaaaagct ccctcttcat atccaaataa
     5401 ttcactctct aataaattgt ctggtatagc agcacagttt acagctataa aaggattatc
     5461 tactctattg ctatggttgt gtatggctct tgcaaatagc tccttacccg ttccactttc
     5521 cccagtaata agtactgttg atgttgattt agaagctatt agggcttcct ttttagaatt
     5581 tcttatagct acactttctc caattatgtt ttccaatttt attctataat ctttattcat
     5641 cttattgtag ttttttatag catctctctt atctataaaa tcaataacat atccctttaa
     5701 ctcatttttt aatactattt tattgatgtt gtatattccc ttcgaatcat atccgtatct
     5761 tttataaaaa aaactacagg atttgtcctt cttaaagtta tttatagaag gtttctttat
     5821 aaaatctaat tcgacaaata tgtttttgcc agttatacta tcttttatat tgaaaagctc
     5881 tttaaatttt gaattatatt tatcaatttt tccatccaca tcaatagaca caacagcttt
     5941 gtccatattg tttaatagtg tctctaattt tttcttttct aattcaagct cataagtttt
     6001 aatttgtgct tttaatttat tagatattag gtctgccatt ttgcctaaaa agtttataag
     6061 accactttta ttattttcta taatttgtgc ttgttctttt gtaaaggcta taaggcctat
     6121 aacaccatga gaatcgccat cacatataat aggacaacac acctctgcaa actctacaca
     6181 agccccactt ttatagcatt ccctacatat atcactactc tttgggtcat caattagtat
     6241 tgtaatctgt tctgtaagtg attttttaaa agccgtatat ttatctactt tctcacctat
     6301 tttgtctata tatactcctg tacctgcaat cctaaataac tccttattta ctatagttac
     6361 atctatattg agtactgata ctatagcttc agctatattt tgaatatcag attttatata
     6421 ttctaaacct atcataatca tcattcctta tttttcttta gcataagtat ttgtcttatt
     6481 tctttaagaa tcaagtatat cttatttaaa gtaaattgga ttgacatcag gattacaaag
     6541 agcataacaa ttattattaa ctcataattt atttccatct aaacatctcc ttataacaaa
     6601 agactaggct taacctagtc ttttatatta tatccatatt tatattttaa ctcatttatc
     6661 ttctttttcc cacataaata tattttaatt tatttgactc tttaatcata ccatcaaagt
     6721 ttaatcttga aaatatatta aatacttcat gtggatagtg aatctttgca acagaaccac
     6781 aaaatttatg tttaagatag tttaacttaa cttttaaatt ttttaaattt aaatcctcta
     6841 atataacctc tccattgccc tttaacactc tttgtatttc agatgaagct ttttcacaat
     6901 ctgtaataac attaaattgg tcaactatta ttatcttgtt gaactgatta tcatcaaatg
     6961 gtagaaactc taacttacct ttaactacat tgcaattttc attatacact agagaataac
     7021 agacatcttc ataattacta ttctccagta tagttacaga agttccaaga cctctcaatc
     7081 tcttgcctag gtttcctata tgaccaacaa gcaatatttt atcacctaca ttatactcaa
     7141 ttgtatccaa aaaacattct aattcatcag atgtatattt agctttaacc ggctgtttat
     7201 ttatctgaca taatatgctt ttcacaatgc ttcacctctt catttcctca attataaatt
     7261 atgtacagat tacattataa taaatggcga gcaactattc aagagatatt tcattagtat
     7321 ttttttaaac aatctttata cattttaata tgatattata ccaaatttat taggatttta
     7381 ttcataaaaa tcagtctatt ttttatgttt attcattgtt attttcatcc aattttttta
     7441 ttttcgatat ggatacttca tatgccattt ttttgaccac attattttca tctatttttt
     7501 tctcatattc tctactttga accctacccc aaagctgtat tctatcacca acctttagat
     7561 tctttgcaaa tttagcattt ctaccccaaa ctattgatgg tatataatca gatttattat
     7621 aaggtctatt tatagcaact aataaatcgg ttatttccct tcctaaaggt gtcttcctat
     7681 acacaggttc cttacataca tatccatcta aaaatatact attagggtct ttattttcct
     7741 cgtctatgct ttttatttct ctgacaaaca ctgtaagcac taatctattt ttattattta
     7801 atgttttgtt atatgatctt aattggccta cgacattgac taaattttgt ttatcaaggt
     7861 caatatcttg gagaagtctt tctgatacag tcattggaag tatatcaaaa gaatcgctta
     7921 gtctgtttat ttttattttc atattataaa atttttctcc aaatatctca tggctaaatt
     7981 ctagcttatt gtctaactcc cctcttaagt ttactacgtt attctcatct ttaacagcta
     8041 tcatatttta tccctccaca attgttaatt cttatatttc tggtatcagc ttatataata
     8101 aatatattta taagggatgt attttattac aaatctttga ttgctgttca aaagttttta
     8161 tttagttata aatttcattt aatatttcac ttttataatt ataaaattat tatctttttg
     8221 taccacatat actaattaaa ctacctattt cagttttgta ttaa
//
LOCUS       DAEORZ010000164         8069 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEORZ010000164 DAEORZ010000000
VERSION     DAEORZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.168"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(173..928)
                     /locus_tag="KRH39_003303"
     CDS             complement(173..928)
                     /locus_tag="KRH39_003303"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG8513182.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDTELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDLIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1368..2357
                     /locus_tag="KRH39_003304"
     CDS             1368..2357
                     /locus_tag="KRH39_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBG8513183.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2809..3525)
                     /gene="dapD"
                     /locus_tag="KRH39_003305"
     CDS             complement(2809..3525)
                     /gene="dapD"
                     /locus_tag="KRH39_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBG8513184.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     NNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3749..4498)
                     /locus_tag="KRH39_003306"
     CDS             complement(3749..4498)
                     /locus_tag="KRH39_003306"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG8513185.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4565..5452)
                     /locus_tag="KRH39_003307"
     CDS             complement(4565..5452)
                     /locus_tag="KRH39_003307"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG8513186.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5569..6570)
                     /locus_tag="KRH39_003308"
     CDS             complement(5569..6570)
                     /locus_tag="KRH39_003308"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBG8513187.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(6995..7876)
                     /locus_tag="KRH39_003309"
     CDS             complement(6995..7876)
                     /locus_tag="KRH39_003309"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG8513188.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
BASE COUNT         2767 a         1289 c         1040 g         2973 t
ORIGIN      
        1 aaacaatata attccatata ccaataaata agataatctt taagaaaaat gattatattt
       61 aaaaggtaag cataaaattt ataaactttt agaatagaaa tctcaatcaa ttagatattc
      121 aaattgatga atatgaatac ctaaatagat tgagatttat tatgtacaaa acttatccta
      181 atatatctga cattttataa agcccagatt gcttaccaat taaaaattta gctgcaacta
      241 tagaaccgtt tgcaaatact tcatcagatt gagaatgatg gcttaatgta agaacttcat
      301 tatcacctgc aaatataaca tcatggtcgc tgattatatt gccacctctt atagagtgta
      361 ttccaatttc atttggtttt cttttacaat ctctaccata tctaccatgt gtattttcac
      421 tttcaggcaa gacttctcta attgcattag ctatcatagt agcagttcca ctaggggcgt
      481 cttcttttct attgtgatgt ttttctatga tttcaatatc aaaaccttct aacatctttg
      541 aagcatcctt aactaatttt attaatatat tcactccaag agatgtattt gatgataata
      601 atataggaat gttttttgat gcttcttcta ttttatttaa gtcattttca tcatatccag
      661 tagtagctaa tactacaggt gtattatttt ttagagcata tgacaatatt ggctctaaag
      721 tctcatgatg tgaaaagtct attattagat cagttttttc agttacatca gtcattgttt
      781 tgtaaagctt tgtatcacta tgatgacctt tttcatcagc agacctagct acaccacaaa
      841 ccaactctaa ttcagtatct ttttttacac attctgctaa aactcttccc attgttccat
      901 tatagccact aattataact ttcaacataa attcctccta tgaaattaat aaaaatttgt
      961 tatatttcta taagtttatt ttatcactaa tataatttat ataaaataat attagtgata
     1021 ataagcttac aatttatctt acattaaaaa atttaagtgg atttttcaaa atatagataa
     1081 atttcttttt cattgtataa atgataaaaa tatatttgac tttataccaa aaagagtgcc
     1141 ccatgaacta aaaagttaat tttgaaatac catattcata tatcatatga tttatttgga
     1201 aatcaatatt aatacattaa aataaccaaa tacatgtatt cataaagatg tataaaaaat
     1261 atataaacat atagtattaa ttttaaattc aacaatttaa ttgttaaaag tgttattatt
     1321 aaattagaga tatcttaact taatatactg agatgggagt gatagatatg ttaaaagaag
     1381 aaaaaactta tcaagagaaa gacgcaaaag tattcaatgg agtggcagct ttgataatga
     1441 ttttattatt tttagtagtt tcaataggta tcatggtttt tgggatagta aaacttgata
     1501 gtggatttac tggaataggt tcacttatgt taattttggg ccttgtattt atagtagtag
     1561 attttatcct attttttggg ctaaaaatga ttaatcctaa agaagctata gttctagttt
     1621 tgtttggtaa ctattatgga actataaaaa aagaagggta ctattgggtg aatccatttt
     1681 gttcagcaat aaacccagca gtatcaagaa ttaatgtatc gaaatcttca tctgatgaaa
     1741 aggtagatat atcaactaat tcaaggggca aaaaagtatc tttaaaaaca atgactctta
     1801 ataatgaaaa gcagaaagtc aatgatttat tgggaaatcc aattataata ggagtggttg
     1861 taatttggaa ggttatagat gcaacaaagg cagtatttaa tgtggataat tataatacat
     1921 tcttatctat tcagtgtgat tctactattc gtaatgtttc aagactttat ccatatgatg
     1981 tatctgaaga tggagatgaa aaatctcttc gtggtagtag tcaggaaata gcagatagat
     2041 taaaagatga attacaatca agggttgata ttgcaggaat tgaagtgtgt gaagtgagaa
     2101 ttactcatct ttcttatgca ccagagattg cagcagcaat gttacaacgt caacaagcag
     2161 aggctataat agcagcacgt aaaaagattg ttgagggggc tgtaagtatg gtagagatgg
     2221 cattgactaa tttaagtgaa aataatgttg tgactttgga cgaagaacgt aaagcagcta
     2281 tggtaagtaa tctattagtt gtgctttgtg gcaataaaga tgcacaacca gtagtaaaca
     2341 gcagttctat ttattaaatt ataaaaagtt taatagttta ttttattgat atatagaaaa
     2401 tttagtagtt cattttatta aattatagaa agattaatgg tttgctttat tgaattataa
     2461 aaagtttaat aatttattct attgatatat agaaaattta gtagttcatt ttattaaatt
     2521 atagaaagat taatggtttt cactttatta aattataaaa agtttaatag tttattacat
     2581 tgatatattg aaaaattgaa ggaaataaaa atacttgtca gcaagaaata aaaatgatat
     2641 aagaatgaat aagagaattg taaaaaaata aaatgattat aaataaaaga taaaaatatt
     2701 gtaaaaaaga gggtgcctct ggtgaattaa attcacacag agacaccttt ttaattaatt
     2761 ttttaaatta gtatattatt gtttaattaa tagtatagta ctatttaatt agtctaaatt
     2821 tctaaggtct tccataagct tagttttgtc agccgttttt tcatctttca tttttataac
     2881 tttagcagga gaaccagcaa caacagcacc agcttctaca tcagtagtta caacagcacc
     2941 agctgcaaca acagcacctt taccgattct aacaccttca agaataactg cattagctcc
     3001 tattaaaaca tcatcttcaa ctataacagg agtagcagaa ggtggctcta aaacaccagc
     3061 aacaacagca ccagcaccta agtgaacatt tttaccaaga gttcctctag ctccaataac
     3121 agcattcata tctaccatag aaccttcacc aataacagct cctatattta taacagctcc
     3181 catcattaca acagcatttt tttctatagt aaccatgtct cttattattg cacctggctc
     3241 aattcttgca tgttcactta aagtattttt tagaggaata gcagaatttc ttctatcata
     3301 ttctacatga gtatctgtta tatcatttcc atgtttatct aatacttcca ttatatcctc
     3361 atagtcacct acaaatatat aagaaccatt atttccaaat accttaaatt tatcagttga
     3421 ttggaagtgt aaagagttag tatttacata taccttaaca ggtgttttct ttttaacttc
     3481 ttttatatac cttgctattt cataagcatc gtttaaattt accatttgtt aattctcctt
     3541 gtatttcatt atatttcttt tattatatac ttatttgtca attttagtat atacttactt
     3601 cttattattt atattttatt ttagttagtt tttattgtat agacatagta ttaaataaat
     3661 ttattatata tgtttgattt gtattgcgta ttattatatc tatattgtat atgtttaatt
     3721 tatattgtgt attattatat ttatactatt atcccaacat gctgtccata ttataaaatc
     3781 ctggtttttg cttaactaag tatttggcag cagctataga accacttgca aagataccac
     3841 gagattgagc ttgatggctt attgtaagaa cctcattgtc tccagcaaat agagcatcat
     3901 gttcgccaac aatatttcca cctcttatag catgaatacc aacttcattt ggttgtcttt
     3961 ttgcactacg accatatcta ccataattat tttcaacgtt tggcaaaacc tcttttatag
     4021 cgttagcaat cataacagct gtgccacttg gggcatctac ttttctgttg tgatgttttt
     4081 ctattatttc tatatcaaat ccttctagta atttagctgc atctttaacc aattttacta
     4141 atacatttac accaagagac atattatatg attggaatac aggaataact ttagccgctt
     4201 cacgtatttt gttcatttcg tcatcattat atccagtagt agcaattact aaaggtgttt
     4261 tagtctttaa aacgtaagat aagatattat ctaaattaga gtgatgagaa aaatctataa
     4321 ctacatcagc ttcctcaact atagtactca tatcttcata aattttaagg tcgccttcat
     4381 agctatccag acctctagct gcaacacaaa ccaattctaa ttcattatct tctcttatag
     4441 tatcggctaa aatcttaccc atttttccat cataaccagt tgctataact tttaacataa
     4501 tttcctccta tgattaaggg ctaacttttg accaaactta ctttttacca tgcattaatc
     4561 tttactatgc attaactttt agtccaaagt taactaattc tttctttaac acttctaaat
     4621 ttgctgggtc catttctgct agaggtaatc ttaaatctcc aacattgaat cccaataaat
     4681 tcattgcagt cttaactggt ataggattta cttctataaa taaagctgcg attagagcat
     4741 ccataccaag ttgtaatttt ctagaacctt caatatcgcc ttcaaagaat ttagctacca
     4801 aatcgtgagt ttcttgaggg caaacattag caagtactga tataactcct tgacctccta
     4861 aagataataa aggaagtata gtatcatcat taccagagta gatagcaaag tcatctggaa
     4921 ctaaactagc tatttctgca acctgtccta ggtctccact agcttctttt acagcaacta
     4981 tgttatcaat tttagcaagt tcaactataa ggctaggact tatatttaca caagttcttc
     5041 cagggacatt gtaaagtata attggaagtt taacactgtt agctattgtt tcaaaatgac
     5101 gcttaagtcc agctttatta gctttattgt aataaggtgt tattattaat agagcatcta
     5161 ctcctaattt ttcagcttct tgacttaagt taactgagtg catagtatca ttagaaccac
     5221 ttccagctat aacaggaact cttttattaa ctttttcaac aacatactta atagtagcaa
     5281 gttgttcttc atcagtcata gtattagctt caccagtagt accacaagat actatagcat
     5341 cagtaccatt agctatttgg tattcaacta attcacctaa tttgtcaaaa tcaactttgt
     5401 tatcctttgt aaatggtgta actagagcaa cagcagaacc tttaaatatc atataaaatc
     5461 aatcccctct tttttatttt tgtgttttat attacagtta cttctaatgc ttgtttttga
     5521 gttactatta atattctaca aaattatata tttatacaag tttgtatatt atactaaatc
     5581 gtatgataaa gccatttcag ctatttgaat tgcgttagtt gcagcacctt ttcttatatt
     5641 atcagcaaca acccaaagat ttacaccatt atcaacacta aaatctcttc ttattcttcc
     5701 aacaaatact tcatctctat catcaaattc tacagcagtt ggatattcgt ttttagcagg
     5761 attatcaact actacaacac ctggagcttc ttcaagtgtt tttactaaat catctaactc
     5821 aaatggattg ttaaactcaa catttataga ttcactatga ccatttttta ctggaactct
     5881 tacagtagtt gcagttattt ttaaatcgta gttatcaagt attttcatag tttcatttac
     5941 cattttcatt tcttctttag tgtatccatt ttcagtgaat gaatctatat gaggaagaca
     6001 gttgtaagag atttgatgag ggtagaatcc agtaggatta ccgttcattc ctttctctaa
     6061 atcttcaaca cctttaacac cagaaccaga tacagcttgg taagtagagt aaacaactct
     6121 cttaattttg tatttatcat caagagcttt aaggggaacc atagcttgta tagtagagca
     6181 gttaggatta gctattatac ccttatgatt ttttatggct tctggattaa cctcaggaac
     6241 tactaaaggc acatctggat tcattctcca ttgactagag ttatctacta ctaagattcc
     6301 atttgaagca gctattggag catacttttc actaatactt ccaccagcag agaataatgc
     6361 tatttgtatg cctctatcag taaatgaagt ttcctttaat tcttctacta tatattcttt
     6421 accacaaaac attactttag aaccagctga tttagaagaa gagaataaaa atagattttc
     6481 tattggaaaa tttctttctt ctaatacttt taaaaatgtt ctacctacca ttccagtagc
     6541 accaactatt gctacattta cttttttcat ataaattcac cccaatttta aattattaaa
     6601 acattatttt attttagtta tacaagtaag atatgttaat atttttatat atccaaaaac
     6661 tttaatatta tattaacaac tttaattata tacaaaaaac gtacaaaggt atactttgta
     6721 cgtctaattt atcactcaaa gtaaaccctg tagctcatca cctacaaaaa taagtgacag
     6781 tattacacat gttatgtgca atcccagaaa atattattgc aagaaatatt ttcttcggct
     6841 gtaaatcctt tttccaatca ttaacacctg ccagctacta ttggatactc ttagttacag
     6901 ctacctctac cctaggttaa taggatatta aaatttgatt attctaataa taatacatat
     6961 ttaagaaaaa atcaacttat ttatataaat attgctaaaa tttaaatcca aaatttgtca
     7021 attcttgttt taacacatta agattagttt cttccatttc agctagagga agtcttaaat
     7081 caccaacatt aaaacctaaa atgttcatgg cagttttaac aggaactgga tttacttcta
     7141 taaataaagc agcaattaaa gcatccatat caagttgtaa tttttttgag ccttcaatat
     7201 caccttcaaa gaatttagtt actaaatcat gtgtttcttt aggacatatg ttagcaagta
     7261 cagagataac accacttcca cctaatgata atagtggtag tatagtatca tcattaccgg
     7321 agtatatagc aaagtcttta ggtactaatt tagctatttc tgcaacttga gctaagtctc
     7381 cacttgcttc cttcacagca actatattat ctattttagc aagttcggcc acaacagatg
     7441 gctttatatt cactttagtt cttcctggta cgttgtataa aataattggc aacttaacgc
     7501 tatttgctat tgtttcaaag tgaagtttaa gacctttctc attagtctta ttatagtaag
     7561 gagttattat taaaagacca tccaccccta atttttcagc ttcttgactc atatgaacag
     7621 acaacatagt attgtttgaa ccagtacctg ctataacagg tattctctta ttaacttttt
     7681 caactgtata ttttattaca gcaaaaattt cagattcact catagtagtc gcctcacctg
     7741 ttgttccaca aacaactagt gcatctgtgc cattctcaat gtgatattcg attaattcac
     7801 ctaatttttc aaagttgaca ttattatcct ctgtaaatgg ggtaactaaa gcaacagcag
     7861 aacctttaaa taacatataa tcaaatctcc ttttttatct aaatttaatt ttttaagtac
     7921 aataataatt atagtacaaa ttaattaatt ttaataggta tagttaatat cttaactgtt
     7981 taaataagat atttatatta tattaatatt agaaaaattt taaataaatg ttttattctc
     8041 aaaatatgca tattaattta ataaaatat
//
LOCUS       DAEORZ010000165         8017 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEORZ010000165 DAEORZ010000000
VERSION     DAEORZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.169"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..5117)
                     /locus_tag="KRH39_003310"
     CDS             complement(<1..5117)
                     /locus_tag="KRH39_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG8513189.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKALIGNVTEEKIRNILKKAKEDLDSQEKDYDGYKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKRDMGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDDSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKDGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYKWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEDISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNRKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILKSLGYIDFKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVG"
     gene            complement(5132..7627)
                     /locus_tag="KRH39_003311"
     CDS             complement(5132..7627)
                     /locus_tag="KRH39_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8513190.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDERDGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIDR
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNL
                     DVGSNVKINVSGKSGELVDSSMYGKNGEDGRDSGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGKNGETGASGSNGADAGYTSYFHGQMFKGYFAKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGSGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
BASE COUNT         2329 a         1621 c          824 g         3243 t
ORIGIN      
        1 cagcctacca attcaattgt caaattaggt atattttcct ttagtatact agtctgctca
       61 taaataattc tttccaataa atcaacttgt aatatagaaa aatgcttcat aatctcccca
      121 tcactatcta tatctttctt gatttcatac caactctttg acaaaattcc cttaaaatca
      181 atatacccta gactcttcaa tatatctata ctatcgctcc atgcatactt actaggttta
      241 tcaaagtatg actctagttt cttcttctgc tcatttgtaa gtgaatcata agaaagcttc
      301 ctattaaaat gtgccttcaa agaaatctta gccatagaat atggacttct accagagttt
      361 aatcctgcat ttttgaactt ttcaattcct ttatcttcta ctgaagacca tattgagttt
      421 attgaatctg aatcattgta tttaacatca agaccatgtg taatatttat atagttttgt
      481 ggaaatacat attgataata gacatctttt ttatcatcat atagagagaa gtatccacta
      541 tcatataaat gaatatgtac ctttactttt aaactatcct ctaaaatatt tcgtctaagg
      601 ttcgggatat ttgcatacat ggttacatat tttatggcat caaagttttt atttaatgga
      661 acttcaaaac taatttcatc tcctgtcaaa agctttttca cttcttcttc attacaatca
      721 tatagagaat atgtattttc aaatcccaca cgtgatttcc catctaaact gcctgttaat
      781 gctaccttca aaatatggtt atctgttttt tgccattggt aagcatcttg gtcaagaaac
      841 tctctaatct gtctattttg ctctttcgcc tgatttataa cctcgttttc atcttctgta
      901 atccctacat ggcatctatt taagatattt actatattgc ttccaaatgg gaatgtccat
      961 agttgaaaag ctgctgcctc aacctgacac aagtatctaa cctgatttaa ttcaatttga
     1021 tggataagtt ttttctgata ttcattcatc tgattaccac tatctgacat ttgataaatt
     1081 aattctccca ttgcaagatt atcttttttc ttatcaatct tatcagtaat gataatttga
     1141 gtttccatca tatcctgtac ttttttgaag attatagaaa tgtcctctga attttccatt
     1201 tttaagcttc caaactttgt gcttaattca gttaaaactt ccttcatctt taatttactt
     1261 atctcatttt caaaaatatc ttgattttta actgtactat cgaatttttc tacaaatggt
     1321 ataacagatt ctttcaaagt tttatcttta aattccacca tttgagtatt caattcatct
     1381 aatttcttaa tattattttc tttatcttta attgcctctt taactacctc tattttactc
     1441 tcaaaactag attttacatt aagacaatta ttaacaccac taatcgcagt atttataaga
     1501 gaacaagcag aacttccaat actcaatgca tttttaattt tagatgtctt ttgctgtctt
     1561 tcaatagctt gtttactttt ctctagtgca gctaacttat ctttttgctg tttattattg
     1621 taatttgcct ccaacatacc tatttctttt ttctggattt ttgtgtaaat ttccttatac
     1681 tcgctttcac ttaacccctc ttttgctctt tgaatatcgt cagctttgtc tgcatagtta
     1741 tcatcatcta tagacttaac tataatctcc ttaatttggt caaatttagt tcttctttct
     1801 tcatcaacac ctgccaaatt ataagtcatt ttatctaaag cattttttct atcttcactt
     1861 agctgttctc caagtacaaa tctcatacca tcagaatcca atttaaaatc tgaaaaatca
     1921 accatcctgt tagtccattt gtattcctct tctttagctg aaagattttc cttcaaagat
     1981 tttgcactgt caaatgcccc tagacaagct tgaccaacac cactaaatgc agtcagtaaa
     2041 ctcaatgcgc tttgtattcc agaaagaatt aactgtgttt tcatttgttc ctcaagtttt
     2101 gcctgtttct tttttagttc ctctatgtct tcttgttcat cttctttcat ctttataatt
     2161 tcttgaatca acaggttcat ttttgcatta acagaaagca ttttattctc aagttcattt
     2221 tttaaaacct ctatttgttg atttccagat tcaatttgag ttaggatact ttccttatat
     2281 gtattgaaat atccttcttt taactcatct ttcttccctt gttccaattc tcttaaggtc
     2341 ttatccagat aatctgtcaa agaacgaata tctgtaacac gatttcctcc aatttcactt
     2401 tcttcaatca tctgcacata aaatgctgtc ttctgctcaa gtgcaaataa aatcatactc
     2461 cattcgctaa aattctgatt ttttcctaca aaagattcta ttctatcaga ctgcatcaat
     2521 ttatttataa ggtattcata gatttccttt gcctctttat aaaattcttt ctttgtattc
     2581 atatcatcca gagaaagtac tgctttattt ttcttctcaa aactatagta cttctgaagt
     2641 atttctctat ttaattgcat ctctattttc tcaactgcat aaaaaatttc ataaatatgt
     2701 gaaaattcta aaatatcagc ttctaaaatt cctactgcac acaacagtct aagatttcta
     2761 taacctttaa ttccagaaaa accaaatctc tcgaaaaatt catagaagaa tgtatctatt
     2821 gacaaagaat actcctgctt taacaattcc ttttctttta gtgcatctcc agagtgaccg
     2881 tcttttccct ctgctccggc tgaacctgaa tcttcacgtt tgctaattgt ccagtcctta
     2941 tgttcttcat aaagagtatt taatttctct ccatcatctg ctgcttgctt ctgatttaaa
     3001 gcatgattaa acacatatct atcaaaataa tagtagtttc tatcactatc atccattgga
     3061 ggagaggtac ctatatatct tattgtttct tcatatatac ctctaattcc accaagacct
     3121 cctgcaccac cagctcctcc agagcctcct tttcctgcta aaccatgatt tccatctttt
     3181 ttacttatag cactttttaa taaaggagct tttccaagga gataaacctg tccagacttg
     3241 cttccaagac ctgcatttcc accattacct cctgtgcctc cagttccacc ttgttttcct
     3301 ttttctgctt ttaccacata gtattttgta cctttaacat tagcaaattc ttttgactct
     3361 tttttagaac ctattttagc atttgcccca gcttctccag ttatccctgt atctcctttt
     3421 tttccatctt gtccatttcc accacgtcca ccatctacgt aaagtttaag ttctcctctt
     3481 ttttctattt ctttaaaaga taatatgata tcacctgaag tacctgcatt atttccattt
     3541 cttccattca ttccgtcgta aatagaactt tctccaacca atttttctgc atccttacca
     3601 gatacgttga ttgaataagt cccttctggt atatgtaatt tttctgtaag tagtacaata
     3661 ttcttacctg agaagacttc tttctccata ttacagtcaa ttttcaactc acaatctcca
     3721 tacatatata tagatgacaa aatattaagt tgataaccag atattatttt agtcaaatcc
     3781 gataaattca ctttaggtat aggaatatat atagaaagta cattttcttt ctcatatgat
     3841 tttatatatg tacaactttg aatcttctct atcctatact ctgcatcttc aagctttttg
     3901 gcttcttctt ccctatattc ttcttctgct atccaatatt tgatatcttt taatgtatct
     3961 tctaaagtat tttttagcaa tgattttaac aactcactta gaccaacaat atgagtctca
     4021 ctacaataac tatttaactc taaaagaaga cgtgtttctt ctaatatatt cactttctgt
     4081 actccatcac catatgaaag ttcttgtaca aactccataa gctctttcat attattagtt
     4141 ctgctacata aatctgtata catcttgtcc aattgaatct ttttatcctt tagactattt
     4201 atagaagata gaatggaaat agaatcttcc ataccttttt tgacactttc tatataagga
     4261 actaaaagtt tttctaggtc tttataggtt acatcaacgg ctttttgtat ttctatttta
     4321 caagaagcag taagcatcaa tcccatatct ctcttttcaa aagtttcagg gagatttttc
     4381 atttcatcca acaacttttc tggctgttta tctagtggat tacataattt aatagataca
     4441 ccatctgata atgctacatt taataagtac actaattcct tataaccgtc gtaatctttc
     4501 tcttgactat ctaggtcttc ttttgctttt ttcaatatat ttcttatttt ttcttctgtt
     4561 acattaccga ttagtgcttt aggtatttta ttgataagaa agaagaaatt ttctttatac
     4621 tttgcataat caccaagcat atctttcaaa tttttgcctg tttctattaa taatgtatgt
     4681 ctagagtcca gtaaggattg atagtctaaa ataactacca tccctttcac ctttgaagat
     4741 gtagaaatag tttcccttac aagcatagca ccagcaatca tatactctgc tccacggtta
     4801 tcaaaaaaac ctgggcaatc acataatgaa aaatctctat ttgcaatatc tacaacttct
     4861 ggacataaag tcttagaata tacaccattt ccaatttcga cagctgaatc ttttgcagtt
     4921 atatatattt gtccagtctc ttcatccgtt ttttcttcca tcacacagcc ttttaaataa
     4981 tttatacaag tactctttcc aacgcctgaa gcacctagga ataacaaaat tgacttttct
     5041 tttaagtcat attttctaag ctcgtctctt ccttcttcca aaaggtttac gatttcacta
     5101 taaagtttat ttgccataca tccatccctt tctatatttc ggaacaatag ccttgatatt
     5161 tttctttaat aagattctct tctacctttg aaacagctgt tgataactta gtgtttggag
     5221 cattaaatcc tccattgtct cctctatctc catattcagc gtattctctt gctggtcctg
     5281 ttttaccttc attccaactt ccaccactaa attctatatg atatttaatt atactatcca
     5341 acttataatc ccaacttgta aaatatcctg tatagtacac agtcttatct ataccacttg
     5401 gagaaaatga aagtgtattg tatccgtgat agccaccact tcctccatat ccacctgaac
     5461 ctggtctacc atttggacct gcttctccat ctttcactgt tatatcacaa tgattaatta
     5521 ttctattttt ttgttcctct gaatctgaaa tgaagtaaat attacctttc ttaccagcat
     5581 ttccacctct tccagcattt ccaccagctc cacctttttc accttttttg ccacttgtac
     5641 cttttgcaaa atacccttta aacatctgtc cgtggaaata acttgtatac cctgcatcag
     5701 ctccattact tccagaagct cctgtttcac catttttccc atctattcct ttagaaccat
     5761 ttcctccatt tgcaatcagt gttaaacgat aatttctaat ttcttcttta atatataagt
     5821 aaatattacc tgaatctcta ccatcttcac catttttacc atacatagaa ctatctacaa
     5881 gttctccgct ctttccactt acattaattt ttacattact acctacatca agattgttac
     5941 aagcaagaat aatatttttc tctgatgcaa agtccattcc aaggttatca tccatataaa
     6001 tagtatcttt agcaaagaac ataaatgtag aaacattgtc atctactttt ctatttttga
     6061 aatattccaa tacactgcta attactaagt ttggaattaa tgctcttatt tcaatacctt
     6121 tcttatcatt aatattaatt gtttttacta taaatggatt taaaatgtat ttaccaataa
     6181 attctctaat tatctcatta ccttcttctc catatgcact taatcttgtt atactgtctt
     6241 ctaactcttc attttgtgta actgtaacat ttgtcaaaat tgaattaaaa tcacttacac
     6301 tatatacata atccttttta tttgcaactt ccattaattt ttctctattt ggtaatgtaa
     6361 atacctgttt ttgcacttca taagaaacta aaactttttc taaattcttt cttattataa
     6421 tgaagttttg ttccattatc ttcttacttg ccttttctat aataaattcc atatgcacag
     6481 aaggtaataa aatttcccca gttccatgtt ctgtatattt acttaaagca tcaattatat
     6541 ctaagtttcc ttttatctta ttttgtaatt tcatgcaact atcttgtaag aataagccac
     6601 tttgtttggc taatacgttg ataaatctgt ctatagtaag acttgttgta ctttccaacc
     6661 agtttttaaa tacttttaca acctcatcat attttgctat ttcttgaatc attgttatac
     6721 ttccaataat actgataaaa tcttctgtat acgccttaac ataatcttct aggctagata
     6781 cgatattgtt ttgaatttct tttatcaacg tatctaaaac taatcttgca tctgcactaa
     6841 ttggatactg gaatgctgat gtcttatctt taacttcatc taattctagt acttgcttta
     6901 ataaaccgtc cctctcatct gatgcaagtg gcttacaaag atgaatattt tttccattat
     6961 cttttagcaa ttcttcaaat aaccaagctt tttcctctat attactgtct aaattttcgc
     7021 ataattgttc atattttctt tttataacat tatatatttg tttttctgtt cttcctaaga
     7081 aactttttga aataaaaaaa gttatactac ccttaaaatt ttctttatga agacacatat
     7141 ttaactgttg gaacaggtct agtacttttg taagtctaga atctaatata tctgcttctg
     7201 gtatcaatac aattactgca catatagatg cagaattttt aaatacttca cctaatgaaa
     7261 ctgctgcgct aatatcatgg gctactcccc tattgtcgct aaaacctgca gtatcacata
     7321 attttgcatc cattccttct attggttttc tcatatttac aatgttcggg tataaagttt
     7381 gagaaacagc tcctgaacca atctctgcca cagggtctaa gcaatcaata tatgatatac
     7441 cagtatcttc atcttcgact tccatcatta cacatcctgc aagatagtta attgcagtac
     7501 ttttgccaca tcctgtccca ccaagaatta gaattaaatt ttgattttca gccttattta
     7561 ttgctacttt tccttcattt aataagagtg ttatttcttc taaaagttga ttgttatttt
     7621 ctggcattta ttttccccct tggttatata ttttatttaa ataaagttta atcccttaag
     7681 tagattacaa agtttttaga ctatatttaa atttgttttt tattttttat ttttaataaa
     7741 tttgcatttt cactaaattt gtattttcac tcaataatta ttcttctttg tttattaaaa
     7801 taactgtgtt gtataatata atctttacca acacagttat tctatttaat gcgcttattt
     7861 tattgtattt tatgtaatac agttgttttt tatattatat gtccattatt ttcctatggt
     7921 tattacatat agatttttaa ttatatttgt accattaatt taaacattcc tatactactg
     7981 cttaagtttt acacctatac cagcttgaat attttat
//
LOCUS       DAEORZ010000166         7989 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEORZ010000166 DAEORZ010000000
VERSION     DAEORZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7989
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.170"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..168
                     /locus_tag="KRH39_003312"
     CDS             <1..168
                     /locus_tag="KRH39_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HBG8513191.1"
                     /translation="SGEYDYLIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTI
                     KEEFNTIKDIF"
     gene            217..801
                     /locus_tag="KRH39_003313"
     CDS             217..801
                     /locus_tag="KRH39_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBG8513192.1"
                     /translation="MKILVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(806..1129)
                     /locus_tag="KRH39_003314"
     CDS             complement(806..1129)
                     /locus_tag="KRH39_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8513193.1"
                     /translation="MKKCLDSYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLEKL"
     gene            1281..1799
                     /locus_tag="KRH39_003315"
     CDS             1281..1799
                     /locus_tag="KRH39_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG8513194.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(1975..2643)
                     /locus_tag="KRH39_003316"
     CDS             complement(1975..2643)
                     /locus_tag="KRH39_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513195.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLILKNSTIKN
                     IIEQHPEYNRKEKIKTEPASSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKIELPLKIDLANGSEHFYGIGGKEQILTKDDLFLSDNIGI
                     LSSIINGPDNRTAITKDTKNIMYFVYGPDKISEKQIQSHLNDIKYYISSAFPDLKVNS
                     IDIF"
     gene            2758..4173
                     /locus_tag="KRH39_003317"
     CDS             2758..4173
                     /locus_tag="KRH39_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG8513196.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHQVDFKLNYTFRNEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLVSQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELNPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQCAIENILEYGHEIILGYAHLS
                     FDEIEKGVRILSKIIHMNQGT"
     gene            4396..4854
                     /locus_tag="KRH39_003318"
     CDS             4396..4854
                     /locus_tag="KRH39_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8513197.1"
                     /translation="MEIIDYIEENLTENLNLDKIADIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEVNSYLNFNINCEVEVA"
     gene            4851..5435
                     /gene="clpP"
                     /locus_tag="KRH39_003319"
     CDS             4851..5435
                     /gene="clpP"
                     /locus_tag="KRH39_003319"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG8513198.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATAGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDRILSENTGK
                     SIEDIRRDTDRDNFMTAIEAKEYGLIDYIMNRNE"
     gene            5747..6823
                     /locus_tag="KRH39_003320"
     CDS             5747..6823
                     /locus_tag="KRH39_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG8513199.1"
                     /translation="MITISLCMIVKNEEDVIGRCLECVKDIVDEIIVVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYIMWLDADDIILEDDREKLLKVKETL
                     DTSIDIVMAKYNVSFDDNGKPTLSYYRERLFKRSSNHKWVDPIHEVIPLYGEIFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKVLEPRHQFYYSRELYYNARYEEAIEGFTKFL
                     DSSRGWLEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEVCCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            6890..>7989
                     /gene="bclA3"
                     /locus_tag="KRH39_003321"
     CDS             6890..>7989
                     /gene="bclA3"
                     /locus_tag="KRH39_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBG8513200.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLRGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGATGATGADGVTGPTGATGATGFGVTGPTGPTGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGATGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTG"
BASE COUNT         3012 a         1018 c         1480 g         2479 t
ORIGIN      
        1 tcaggagagt atgattactt aataaaagta ttagcagaag atacaagtaa tcttgaagag
       61 tttatatcag tgactttaaa ggaaattggt gggattcaga aagctaatac aaccattgtt
      121 ttatctacaa ttaaagaaga atttaataca ataaaagata ttttttaaat aattgactag
      181 taaagtgtta attatatttt aggaggaaaa tagaatatga aaattttagt ttttttagca
      241 aaaggttttg aaactatgga atttagtgta tttgtagatg ttatgggatg ggctcgtaac
      301 gattatgggc atgatattga tgtagtgact tgtggattta aaaaacaagt tatgagcaca
      361 tttaatatac aagtattagt tgataagacg atagaagaag tatgtgttga tgactatgat
      421 gctctagcta ttcctggagg atttgaagaa tttggatttt atgatgaagc atatgattca
      481 tcttttttaa atctaataag ggagtttaat tcaaaagaaa aaataatcgc ttctatttgt
      541 gtggcagcat tgccagttgg gaaaagtggt gttttaaaaa atcgtaaagc tactacatat
      601 catttgaaaa atggtaaaag acaaagacag ttaagcgagt ttgatgtaaa tgtagttaat
      661 gaaccaattg ttgtagataa aaatattata acctcatatt gtccagaaac agctccacat
      721 gtagctttta agcttttaga aatgttgaca tctaaagaac aaatggatga agttaaatta
      781 gcaatgggat ttaaactgta atggtttaga gcttttccaa gtaatcagaa ccccagtcac
      841 acatggaatc taaaattgga ataatactct ttccaaaatc agatagtgag tattctactt
      901 ttggaggaac aacaggataa accactctta taattaaacc atctgattct aattcacgta
      961 attgttgagt taacatctta ggtgttgctt taggaattaa tttttttaat tcattgaacc
     1021 tcaatatagt gtctttaaga tgccaaagta taagtgtctt atatttacca ccaatcaatg
     1081 caagtgttgc ttctattggg caactgtaac tatctaaaca ttttttcatt tttgggctcc
     1141 tttatttatt ttaaagtatc tttttaggta ctatattacg aaaaagtgca ttattgtaat
     1201 atagtaccat agtgataaaa ttatagcata ggttaaataa atcatcaact tagcctaata
     1261 aaacaatagg aggtaaataa atgaatttcg ttgaacttgc aaaaaaaaga tattcttgcc
     1321 gtaattacca agatagaaag gttgaaaaag aaaaacttga gaaggtttta gatgttgcaa
     1381 gaatagctcc aactggtggt aatcgtcaac ctcaaagatt gattgttatt caggaaaaag
     1441 aaggtataaa taaactttct aaggctgcca atatatatga tgcgccatta gcaattcttg
     1501 tatgtggaga taaagacaaa gtttggacaa gaccttttga tgggaaacaa cttacagata
     1561 ttgacacaag tatagttaca gaccatatga tgttacaggc aactgaactt ggattggcaa
     1621 gtgtttgggt gtgctatttt aatccagata taattagaga agagtttagt ttgccagata
     1681 atctagaacc aataaatata ctattgatgg gatatgaatc taagatacca gagagcccag
     1741 agagacatga aaaaactcgt gttcctttaa gtgaaattgt ttcttatgaa actctttaat
     1801 tataaaatta agttattcat aggataaatg taataaagtg tttattataa acaaaattca
     1861 aaataaataa tatctacata taattaattt tagtgaagta tatctagttt aactagaatg
     1921 tatacttcac taatttattt attcaaaata tttatgagga ttttaatata ttaactaaaa
     1981 tatatcaatt gaattaactt ttaaatctgg aaaagcactt gaaatatagt attttatgtc
     2041 atttaaatgg ctttgaattt gtttttctga tattttatca ggtccatata caaaatacat
     2101 aatattttta gtatcttttg ttatagctgt tcgattatca ggtccattaa taatgctaga
     2161 taatatacca atattatctg ataaaaataa atcatctttt gtcaaaatct gttctttacc
     2221 acctatacca taaaaatgtt cacttccatt tgctaagtct attttcaaag gtaattctat
     2281 tttatcaagg tcatgacctg ctgttagaag aagatttttc aattctgcta aaaacattga
     2341 ctcaattgtt acacctacat ctgggatacc ttttgatttt aatagaatag attctaattg
     2401 aagcaataca gggtaagttt tcttgaattt tttataatac tttatataac ttgaagcagg
     2461 ctctgtttta attttttctt tacgattgta ttctggatgt tgttctataa tatttttaat
     2521 tgtagagttt ttaagaatta aaaaattttc tttattaggt tttgaatgtg taatatttat
     2581 aaccataact ccaaatttaa tattgggata aatttcttta actttatgtg atatttctaa
     2641 cataatatag actccttttt attttttatt ttatgatata atcttagcaa gttaaaagtg
     2701 gattttgtag ttccattttt atagtaattt tatagaacca cttttggagg tattagaatg
     2761 tggattacga ttaatagaaa tagcaatata tcattatcaa gacaaattta ttgtgagata
     2821 aaagatatga ttctagatgg aagcttaaag tttgagcaaa aattgccatc atctaggact
     2881 ttatcaaaag aactatctgt atctcgtaat acagttttag atgcatataa tcaattaatt
     2941 gcagaaggat atttggaaac aagaaatgga tatggaacaa ttgtggctaa aggaatatca
     3001 gggcatcaag ttgattttaa gttaaattat acttttagaa atgaaaaaga tttacatact
     3061 aataaacaaa aaaattatat agattttcgt tcaggagttc cagaattaaa actatttcca
     3121 cgcaaagaac tttcaaagct atataaacaa atactgtatg atttatcaga ttctgatttc
     3181 agatatggaa gtacagctgg tgtatgggtt ttaagagtgg aaatatctaa gtatttattt
     3241 cgtactagag gaattagatg taatcctaaa aacattatga ttgtttctgg ttcaacacaa
     3301 ggtttatctt tagtttcaca gcttttatac aaaagtaata aaaaggttgc tgtagaagac
     3361 cctattcata aaggcttatt aaaggttatt tcatctattg gatattcggt tgttggaatt
     3421 aaagtagatg ataagggaat gaataccaac ttaataaaat caataaaggg gatttctttt
     3481 gtttatacta caccttctca tcaataccca ttaggaggga ttttacctat tcaaagaaga
     3541 ctagagctta ttaaatgtgc aacagagaat gattactata taattgaaga tgattatgat
     3601 agtgaattta gatatgaggg tcaaccaatt agttctcttt atgaacttaa cccaaataaa
     3661 gtaatttaca taggttcttt tagtaaaata ttatctccag ctattcgtgt tggatttatg
     3721 ttattaccag acaatatttt agaaagctat aaaaccttaa aaatgtactc tgatgttcat
     3781 acagaaacaa tattgcaaca tgtattagct gaatttattg gaaatggagg tttagagaag
     3841 tacatattta gaatgaagaa gttgtataat agaaaacgta agtacctcat aaaagtttta
     3901 aacacagaat ttaaaaatga atttaaaata aaaggtgaag cagcagggct tcatatagtt
     3961 gctcatttct ataatgttaa atttacggaa agtttaattc ataaaatttt tgaagcaggt
     4021 ataaaagttt atcctataca acaatgtgca attgagaaca tattagaata tggccatgaa
     4081 attatactag gttatgctca tcttagtttt gatgaaattg aaaaaggtgt aagaatactc
     4141 agcaaaataa tacatatgaa tcaaggtaca tagataaaac atgtagcaat ctacatacaa
     4201 tagatgcaat taagtttaaa ctttaaaaga aatatatatt gatatttttg tataaaaata
     4261 cctgatattg tgttatcgat actttgttgc tatacactaa attaagatag caatttatat
     4321 taagaaaaaa ctttttgcat aagttataat acttaaaagg agaggataga gttgaaaaca
     4381 aatacagatg ttattatgga gataattgat tatatagaag aaaatcttac agaaaatcta
     4441 aatttagata aaatagctga tattgctaat tattcaaaat accatttaca cagaatgttt
     4501 acaaatgttg taggatgtac tatacatcaa tacatacaga ggaggagact tactgaagca
     4561 gctagacaac ttatatttac agacaaatat attatagaca tagctattac agtaggatat
     4621 tctacacatc aatcttttac actagcattt agaaatttat ataaagaatc tccacaagta
     4681 tatagaaata aagatgaatt ttatcctatt caacagagat ttgttttaaa tctaaatcat
     4741 caaattcaaa gcgaaaaatg tatttcttat gcaaatatga attataaaca aagaagagat
     4801 gaagtaaatt cttatcttaa ctttaatatt aattgtgagg tggaagtagc atgagtttag
     4861 taccacatgt aattgaacaa acaggtcaag gggaaaggtc ttatgatata tattcaaaat
     4921 tattaaaaga tagaattata tttataggag aagaaattaa tgatgctatt gcaagtttag
     4981 tggtagctca attattgttt ttagaatcag aagacccaga ttcagatata attatatata
     5041 taaatagccc tggtggctct gctacagcag ggtttgctat atatgatact atgaattatg
     5101 taagatgtga tgtatcgact atttgtattg gaatggctgc aagtatgagt gcttttttac
     5161 ttgcaggagg aacacatgga aaaaggtgtg ctttacccaa ttcagagatt atgattcatc
     5221 aaccaatggg aggagctaaa ggtcaagcaa cagatgtaaa aatagcagta gataatatat
     5281 tgaaaataaa agaacgatta gataggattc tttctgaaaa tacaggtaaa tcaatagaag
     5341 atattagaag agatacagat agagataatt ttatgactgc aatagaggct aaagagtatg
     5401 ggctgattga ttatataatg aatagaaatg aataaagtat caaagtatca tataaaaatg
     5461 tctgttttac aaatatatag agaatattaa atgagagtgt ctcaaaatga actttttagt
     5521 tcatgggaca ctcttttttg tatgaaatta aatatgtttt tattatttca acaattatta
     5581 tcaaatttca agtttaataa attctaatat aaaaaataca taaaagataa gactctttag
     5641 caactttttg tcgttaaaag tttttataaa tttaataaca aaataagata aagtaccata
     5701 tattacttat gtctgcaata tatagaagac aaaggagttg tacattatga ttacaataag
     5761 tttgtgcatg attgttaaga atgaggaaga tgttatagga agatgcttag aatgtgtaaa
     5821 ggatatagta gacgaaatta tagttgtaga tactggttct acagattcca caaaagaaat
     5881 agtctcaaaa tatacagaca tggtgtacga tttcgaatgg atagatgatt tctctgctgc
     5941 aagaaatttt tcattttcta aagcttctaa agactatata atgtggcttg atgcagatga
     6001 cataatttta gaagatgata gagaaaaatt attaaaagtt aaagaaactt tggatacttc
     6061 cattgatatt gttatggcaa aatacaatgt atcctttgat gacaatggta aaccaactct
     6121 ctcatattat agagaacgat tgtttaaaag aagttctaat cataaatggg ttgaccctat
     6181 tcacgaagta atacctcttt atggagaaat attttattct gacattgcaa tatctcataa
     6241 gaaactccat agacaggacc ccctaagaaa tcttaggatt tttgaaaaaa tgatttcaga
     6301 aggaaaagta ttggagccaa gacaccaatt ttattactct agagagttgt actataatgc
     6361 taggtatgaa gaagcaatag aaggttttac taagttttta gactcatcaa gaggatggct
     6421 tgaggattgt atcagtgctt gcagagattt agccacatgt tattacttaa ttaatgatga
     6481 aaagagtgcc ttatattcat tgtttagaag tttcgaattt gatgagccaa gagcagaagt
     6541 ttgttgtgac ataggaaagc atatgtttga tagacaaaaa tataaagaag ccattttttg
     6601 gtataaggta gcattaacaa gagataagaa tgatactaat ggaggattta agtcaaatga
     6661 ttgttatgga tatataccat ctatacaatt aagtgtctgt tatgacaggt taggagaatc
     6721 agataaagct atttattatc atgaaaaaac aaaagaaatc aaacctaacg atagtgctgt
     6781 tttgcacaat gaaaattatt ttagtaaatt taaaaatagt tgatgggtca caatcatact
     6841 aaatttgaca tattatatat agagcttaaa agctcaaata tatcaggagg tgcttttaat
     6901 aatgagtaga aataaatatt ttggaccatt tgatgataat gattacaaca atggctatga
     6961 taaatatgat gattgtaaca atggtcgtga tgattataat agctgtgatt gccatcattg
     7021 ctgtccacca tcatgtgtag gtccaacagg cccaatgggt ccaagaggta gaaccggccc
     7081 aacaggtcca acgggtccaa caggtccagg tgtaggggca acaggcccaa caggaccaac
     7141 cggtccgact ggcccaacag gaaatacagg gaatacagga gcaacaggat taagaggtcc
     7201 aacaggagca acaggggcaa caggcccaac aggagcgaca ggagctatag ggtttggagt
     7261 aacaggtcca acaggcccaa caggagcgac aggagcaaca ggagcagatg gagtaacagg
     7321 tccaacaggt ccaacgggag caacaggagc agatggaata acaggtccaa caggagcaac
     7381 aggggcaaca ggatttggag taacaggtcc aacaggccca acaggagcaa caggagtagg
     7441 agtaacagga gcaacaggat taagaggtcc aacaggagcg acaggaacac ctggagcaac
     7501 aggtccaaca ggggcaatag gagcaacagg aataggaata acaggtccaa caggagcaac
     7561 aggagcaaca ggagcagacg gagcaacagg agtaacaggt ccaacaggag caacaggggc
     7621 aacaggagca gatggagtaa caggtccaac aggggcaaca ggagcaacag gatttggagt
     7681 gacaggccca acaggtccaa ccggagcaac aggaatagga ataacaggag caacagggtt
     7741 aataggtcca accggagcaa cgggagcaac aggagcaaca ggcccaacag gagtaacagg
     7801 agcaacagga gcagcaggat taataggacc aaccggggca acaggagcaa ccggagcaga
     7861 tggagcaacc ggagcgacag gggcaaccgg agcaacaggt ccaacaggag cagatggatt
     7921 agtaggtcca acaggagcaa caggagcaac aggagcagat ggattagtag gcccaacagg
     7981 tccaacagg
//
LOCUS       DAEORZ010000167         7867 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEORZ010000167 DAEORZ010000000
VERSION     DAEORZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.171"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            417..2651
                     /locus_tag="KRH39_003322"
     CDS             417..2651
                     /locus_tag="KRH39_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8513201.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLYFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDINKIKN
                     LTKI"
     gene            2682..3527
                     /locus_tag="KRH39_003323"
     CDS             2682..3527
                     /locus_tag="KRH39_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8513202.1"
                     /translation="MFKEIFLKTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFMDSHNIQLDWEKNYPYDKISNWIFEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3767..5500
                     /locus_tag="KRH39_003324"
     CDS             3767..5500
                     /locus_tag="KRH39_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG8513203.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDNTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLLK
                     ADGKNCYSTYE"
     repeat_region   5892..6644
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5892..5920
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(7174..7284)
                     /locus_tag="KRH39_003325"
                     /pseudo
     CDS             complement(7174..7284)
                     /locus_tag="KRH39_003325"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3186 a          952 c         1016 g         2713 t
ORIGIN      
        1 aattaattat gcaaaattat taatatggta ttaaattatg tataaaaagt taattatgaa
       61 taaaataatt atgcctttga aaaatcatta aacttaaaat taattataaa aaaaactcta
      121 ccttactaac tatacagctc acaatctatc tatttatgaa aaaatatgat ataattgcat
      181 tcaaaaagga aatctttcaa tatactagaa tttaagcatt aactaatttt atctaatatt
      241 attactaaac taaaaaatat ttgtatttgt agcattggag caaataactt aatgtatatt
      301 cattttaaat acttatttaa aatttagcat ttaaattatt tacaaattaa catttaaata
      361 cttctagcat aaatacagtt gtcatcaaaa tttaaagtat cttatggagg aataccatgc
      421 aaaatcataa ttatataaaa atgttaaata aaaaggtaga agtattatct aaggaattag
      481 atagtagaaa ttttaaatta aaaaccttaa atgaaataga ttttttttct aaaataccaa
      541 acagagaatt tatgtaccaa tatattgatg actgtatttc gaaaaatata catataggaa
      601 tcatgttatt aaatatcgat aactttaaat tatttaatga tatatatgga tatctagtcg
      661 gagaaaattt attaattact ctaactcaaa acctattgca ctttattcca gaatcaaagg
      721 gaatagtcgc ttatttaaaa ggagaaggtt ttttaattgt acttccaaat gtatcaaata
      781 aagagttaaa taatatgggt gcaactatta tagagagcgc ccgagaatta aaaataagac
      841 ttcctaatga aaaagaaata tactccaatg ttacatttag tatcggcagc actatctgga
      901 atggtgaacc aaacatgact actttgatgc ttttcaacca agtatatagt gctttaaaaa
      961 aggctaaaag tgaaggtaaa aatagataca ttctatttga ttttaaagat aatagttttt
     1021 acaataaatt agaaaatgat aaggagttct tatcagaaat atcacatgtt ctacaaacta
     1081 aacaaattga agtattttac caaccacttt ataatataaa aaataatact atagttggat
     1141 gtgaggcact tgcaagatgg agacacccta ttaaaggact tcttactcca gataaattta
     1201 ttcctttatt tgaacagttt ggtctgataa cggaattaga tttgcattta tttgaagaga
     1261 actgtaaaaa tataagaaga tggattgata acaatgatgt ttttgttcca atttgttgta
     1321 acttttctcg tctttatttt tttaacccta attttccaag tatgctaaaa ggtattactg
     1381 aaaagtacag cgtctctaca tcaaatttta gcattgaaat aacagaaaat atattaatag
     1441 aaaatacaaa tacaattatt caacaactag ctgaacttcg ttcttatggt ttttctgtat
     1501 caatggacga ttttggttct ggatattcat cttttggtat gttgcaaaat ctaccttttg
     1561 atataatcaa aattgatagg atatttttat ctcataattt aaatgatttt aaaaatacaa
     1621 ccattttgta ctctataata aacattgcaa gagctcttgg gatgcttaca gtatgtgaag
     1681 gtgttgaaac agaacaacat gtagaattta ttaaaagtat aaattgtgat atcgcccaag
     1741 gttattatta tgcaaaaccc atggagtgca aagaatttga aaaattacta tctaaagaga
     1801 ataattcaga gaaaatgttt ataaaatatt ctgatatagg aaaatcttta atagagaaga
     1861 tatttgatgg attttttatt atgcaagata tgaaaaaact aagagctaat gtcactgaag
     1921 acattcaatg gtttgatttt tacaacaaaa ctgaaattac atcttttcct aaagtaatag
     1981 aacattttga aaaacatatt aaaggtaaaa aattttctat tctttacaaa agtataaccc
     2041 cacataatac tgaatcagat tcattattaa ttagtggaga agccgtttta tcagatgaag
     2101 cttctgaaca tcaaaaatat agcaatttct actttagtgc aaattgtata atttcagaaa
     2161 ataaattact cttagcaaaa ttacatttgg atttaattaa aactgctggc tatataaatg
     2221 aatttgaatc atattcaata cctaaaatga caaatacaca tcttgatgaa agtaatattt
     2281 tatcacaatt ttattcaatg ttgcctgtag gtataattcg ctacgattta tttggtgata
     2341 tgattattac ttatatgaat gaagaaatgt ttgatattat aggttataca aaagaacagt
     2401 tttttggtga gatgggcgga aatttaagag caattgttca tccagatgat ttagactttg
     2461 tttataaaaa aagtttggac atgattgaca caaataaaat tgaaccattt ttatatagat
     2521 tcataaaacg agatggtcaa attgtcacag ttatgtatac acaatgtaac ctttctgcaa
     2581 ttgatggaag gtcgattgcc cagggtatgt atgtagatat aaataaaata aaaaatttaa
     2641 caaaaatcta gtacaaagta ttttattttg gagaaattaa tatgtttaaa gaaatttttt
     2701 taaaaacttt tccaggattt ctaattataa gagattctaa ctacagaatt atatttataa
     2761 atgataattt aaaaaactta attaaaagtt atatgcaaga taatccactt ggaatgacta
     2821 atatagaaat agctaagaaa ctccctgata atattgctaa attttttatg gattctcaca
     2881 atattcaatt agattgggaa aaaaattatc cttatgataa aatttcaaat tggatttttg
     2941 agtttaaaaa agatactaca agctattgga atgttttaga atacaaagta gatgttgatg
     3001 aaaaaaccta cataattact atggccaatg atataacaaa actctatgaa gaaaataaga
     3061 gaaatcttca ctactcaatt actgacccat taactggagc atataataga aaatatttaa
     3121 atgatagatt tgacatattt ataggagatt atattgtact aattgactta gataatttca
     3181 aaatgataaa tgactatgaa ggtcataatg taggagacaa gattttatgt gattttgttt
     3241 cacttttaaa taaagaatta ataaattcta cttctattat aagattagga ggagatgaat
     3301 ttattgttat attctctagt gatgttgaca aaaattatgt ttattcacaa ctagaagcct
     3361 taagagaaaa ttttttaaaa gtattctcta agtataagta tctttcattt agttatggtg
     3421 ttgatacagt aaaaagaaat ttaaaattga caatagttga attggataaa aaaatgtata
     3481 aaaacaagga gaaaaacaag aaaaaatttg ataaaaatga ttattaatat tgttatttta
     3541 aaataaatta aaattatctt tcttttttta aacttgtaca ttagtttata aatatgtatc
     3601 tatacaagat attgacatta agtactgaag catatattgt ttcagttctt ttttgttaaa
     3661 attaacttat ttgacaaaga tatcatagtt taacaaagct tttaatttaa atatattgat
     3721 gtataatata attaaaatat aatagcaaaa agaaaggagg aatatcatga gcatactcct
     3781 aaaaaaagca cccaaattag caaaacatat tataacaagt ttttatataa atcgggatat
     3841 tgatgaagta ttaaaatacc tttgtgaaaa tgttacatgg attggaccag gagaacagga
     3901 atttctcact tcttttaatg aaataaaaaa ttatttttat gcaggtcaag atgaaattcc
     3961 ttcttgtgat ataaataatg atatatttga aactgtaagt gaagatgaga atagatgtat
     4021 ggtattagga agatatactg ttcgaactaa agaaaatgct caaatgattt tagaggtaaa
     4081 tcaacgttgt acatttgaga taattgagga tagaggaaaa ttattagtaa agcacatgca
     4141 catatcaaac ccttatggtg aaatgcaact tgatgaatac tttcccacta aaattggaac
     4201 tcaaagttat gattatttgc aaagattact aaaggaaaaa acagaagtta ttgaaatgat
     4261 tacaaataat atcaatggtg gcttaaaagg tagcaatgat gataatacat actcattttt
     4321 ctatgtcaat gaaggattgc ccaaaatatt aggatacaca tatgatgagt tcatggaaat
     4381 gagtggtggt tcagcagtgg gtgctgtata tccaccagac ttacctaaag cactagaaga
     4441 ttgtgaacga tgttttgcaa aaggacctac atattcatct gaatatagaa tccgaaaaaa
     4501 agatggaaca ttaatgtggg tattagattc tggaatgaaa tcgttaaata gtgatggcat
     4561 agtaaaaatt aatagtatta ttactgacat aacccaacta aaaaacatag aatctgaact
     4621 aaaattagaa agagagcgat atcgtattgc tttgcaaaat attacagata tcatgtttga
     4681 gtatgatatt gaaaacgata cccttataaa atatcaacgt gtagaaattg acaaaaaaat
     4741 tgaattagaa aattttgaaa caaaaaatta ctccaaacta ttagaatctg gtaaaattat
     4801 tcaccttgat gatattggaa aactactaga agtacttcat ggaaatcttc gtgaaacaat
     4861 tgagataaga caagtaaatt cattaactaa aaatgaatgg agatggattc gtgttcaatg
     4921 ttcagttata tatgatagtg accataaccc tatcaaaaca ataggcgttt taaaagacat
     4981 aactgaagat aaatctaaat tagaaagctt aattaatcag gctcaacgtg acccactaac
     5041 acaattatat aatcaaagag tatcacaaaa tcttattgaa gagtatctat gtagttctga
     5101 ttccaaaaat aatgatgctc tattagttat tgatattgat gattttaaaa ctgtaaatga
     5161 tacttttggg catttagagg gaaatgaagt tttagttgct gtatcaaaaa tactattaca
     5221 taacacacat gataaagaca tagttgctcg aattggtgga gatgaattta caatatttat
     5281 aaaatcatta acaaaggatt taataataaa aatcactaat gatattttaa atgatgcaag
     5341 taaaataaaa gtaaaggaca atcataaaat aacattaagt attggtattg ccttcactga
     5401 tgattcaact aaattatata aagatttatt ttctaaagct gataaagctc tttatctatt
     5461 aaaagcagat ggcaaaaatt gttatagtac atacgaataa taaagctatt tatacgacca
     5521 agaatgaacc tttaaattta atttcatagt ctaacaatca aagagttgaa gctaatctta
     5581 tagctagtca actagttcct agaaaaaatt taaacaatat atttattgat actgctcatt
     5641 tttctaaaga gcaaatttat ataaaaaatt aaacttttat gtacatacag atactaatga
     5701 taaacattta gcattagtat cttctatctt aaaacaaaat gataatttat ctatataaaa
     5761 aagtgctttg ttgacagagt tcactttttt atataggtac agaatttaag tataaataca
     5821 tgttttattc tatacatttt agttaatgta aaattccttg attgtactct ctaacttttc
     5881 aacattatac tatttacata ccacttagtt aatataaaac gctgaattgt acttaattaa
     5941 tatatcatta taaatatatt tacataccac ttagttaata taaaacaagc atcaaataca
     6001 tttatcattc tgtctataat tttatttaca taccacttag ttaatataaa acattatttt
     6061 tttatcgttt tgaaacctgt ttcttctgca tttacatacc acttagttaa tataaaacta
     6121 tcttttgatt attctgataa aaagtaataa tacaaattta cataccactt agttaatata
     6181 aaacttctgg tgttattaat attcagattc cttacgctga atttacatac cacttagtta
     6241 atataaaaca atgacttatc agctccgcaa atggaagttg aaagagattt acataccact
     6301 tagttaatat aaaacacgca acagcaagag tcgcaacaac attaacaatt tcatttacat
     6361 accacttagt taatataaaa ctttattata tgtcaaaaaa taagattaaa agttctgaat
     6421 ttacatacca cttagttaat ataaaactta ttaaaatcaa tttaccttct tctacagctt
     6481 cgtcatttac ataccactta gttaatataa aactggattc gacgggtttg gaaattttgg
     6541 aaatttacca tttacatacc acttagttaa tataaaacga taaaaaagaa gctttaaaaa
     6601 agagaaaaga agaatattta cataccactt agttaatata aaaccccaaa ataaactggg
     6661 cattttcaat acttacacat atgcaactct cttaaatttg cagtgaacca tgagtagtgc
     6721 aaatgataaa gcttatcata taccctcaac accttatatt tcaagcgtta agctctactt
     6781 tgtaacaaat atcgctcact gcaaaactat ttacattttt attatatcat aaatatatta
     6841 tttttgaata tctataccaa tttgtggtat aataaaaaca agaagaacta caatctattt
     6901 agacgtagag tgaagttcaa atactaatgt ttattctttt tgaatttgat ttttagttca
     6961 aaactaaagt cactcctgcc tgtgtgaact atacccgctg ttttatacag ttaaaacatg
     7021 taaaaatagt tataaagtac tagtgtaact cattagtact ctttttatta tattcatata
     7081 attaatatct atattttaaa gtttactatc taaaaaagag cacgtcaaat aaaaccctag
     7141 atatttaatt tttttagaga tacttaaaat aaattatgct ctctctgtac ttaaatattt
     7201 cctaaattca aatggagaca tagaatttaa tctattttga tatctaaaat tattatagta
     7261 gtatatataa tcatctattg ctttaactaa ttattcatat gtatcaaatt tccttaaata
     7321 gtacatttct acctttaaaa ttccccaaaa tccttccata ggagcattgt caaggcaacg
     7381 accaactctt gtataatata tgtatatcta acaccattaa gaaatatata ttttataata
     7441 tatatttctt catttaatgc acttagaaaa gcaaagaaaa tacagattag tacacttttt
     7501 agagaaaaat aaaattaata catctaaaat attaaaaaat actttagata atctaaagac
     7561 acatctcaaa gtttttaact aaattataaa actaattaca aatgctatgt aacgataata
     7621 aataaaaaat ttacttcgct taagcgacgt gtcgttgaaa tatttcatgt tgccaactgg
     7681 ttcgtaagtt gataagaaaa gaacactaat tatttagagt tcttttctta gctacaagtg
     7741 gattataata cttttaaatc tgtgagaaaa ttgtatataa tagtgatatg aataagtaaa
     7801 gaaaatacaa aaatgacaaa attataaata aaatattata aaatcataag gtcataattt
     7861 gttatat
//
LOCUS       DAEORZ010000168         7841 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEORZ010000168 DAEORZ010000000
VERSION     DAEORZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.172"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(358..2637)
                     /locus_tag="KRH39_003326"
     CDS             complement(358..2637)
                     /locus_tag="KRH39_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8513204.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2908..4362)
                     /locus_tag="KRH39_003327"
     CDS             complement(2908..4362)
                     /locus_tag="KRH39_003327"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBG8513205.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEAYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4526..5395
                     /locus_tag="KRH39_003328"
     CDS             4526..5395
                     /locus_tag="KRH39_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8513206.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5608..7680)
                     /locus_tag="KRH39_003329"
     CDS             complement(5608..7680)
                     /locus_tag="KRH39_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG8513207.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT         2667 a         1300 c          829 g         3045 t
ORIGIN      
        1 tgatttgtat aaattattat gaaaaactct ttttttgata taataaaatt acttacatat
       61 ataacattta aatttataaa ttatcttttt attttttaaa agatgtataa cacacctata
      121 gaaattacta ttattactat ctaaagtatt ttgctataat cccatatact cttacctcaa
      181 aaacattcaa atagaaaaaa tactacataa ttaataaact ttgcccaata attcctaaaa
      241 taatatccta acaatgattt atgtttttat aatagtatat ttttacataa aaaagtgaac
      301 attgattaac aaagttcact tttttataaa tttttatgtt ataattcaca attttattta
      361 atttgtttct tttctattta aactatatct atctaatatt aatttttcat attcttctat
      421 tggcattggt ctagcaaata aaaatccttg agccatatca caacctataa accttaaaaa
      481 ctctgcttgc tctacagttt caacaccttc tgaaacaact tttatatcaa gttgctttgc
      541 catttgaaca atacttgaaa ctacaatttg actctttttt gcatttccca ctgttataaa
      601 gaattgccta tctaatttca atacatcaat tggcatatct ttaagcatat ttaaagaaga
      661 ataaccagaa ccaaaatcat ccatagaaat tttaaatcca acagatttca aagtttgcat
      721 tattttaaat aatatttttg tattgtcaaa tattgcactt tctgtcaatt caatctcaat
      781 taaatttggt tcaatttcat attcttttgt tattctttca agctcagaaa taaagcctgg
      841 attctcaaga tgtactcttg ataagtttat tgatatagga aatgtgggga aattttcatt
      901 ttcccaacgt ttaaaatttt tacaaacttg ggtaaataca aacatatcca attctgttat
      961 aaagccattt ttctcaaaaa taggaataaa tacacctgga cttattagac cttttttagg
     1021 atgttgccaa cgtacaagtg cttcagcacc tacaatttta gtagttgata aatcaacctt
     1081 aggctgtaaa taaactttaa attgttcatt ttttaaagca tcatgcattt cttgttcaat
     1141 atcacgttct tctatgagag tttcttttaa attttcagca taaatctcat aaacttttaa
     1201 atattttctt tttactgtag tttttgcaat catagctttg tcaagacagg tagaaatatt
     1261 tgtttctccc tcttcaacaa aatatacacc tatggatgga attaaattga gttctactcc
     1321 caaacttgtt tttatcattg acaatttttt acgtataagt tcacatattt ctataattga
     1381 ctctctatta tttttctttt caaatattat agcaaaattg tcatttgaaa ttcttgaaaa
     1441 tacagattgc tctttaaata tattcttaat tatctctgct atttccttta atactctatc
     1501 tccttcttca tacccaaacc tatcatttac tagcttaaac ttatctatat caaaataaat
     1561 taaaactaga ttctttttct catgagagga taaaaatttt cccccttcaa gattaaattt
     1621 atttgaattg ccaatacctg taacgttatc tttaaaagca aattcttcca atttttcctt
     1681 actcttttta tgtataaata gtatataaat tgttaatgcc gtaaagatta taatcaagac
     1741 tacacatgct gataatgtaa gttttataat agcatatgat ttctcagata tagcagtttt
     1801 aggaacttca gaaaaaagat accaatcatt gattcctatt ggggcataac ttaaataata
     1861 tccttttcca aacatactat aacttaatga acctgattta aagtttttta tatttgactt
     1921 catattgttg atactagatt ttgatattga atcacttctg ccaatgtaag aaaataaatt
     1981 ttctttagta gagttggcgt ttttactgct tggatttaaa acaatatcac cattacttct
     2041 agttatataa gaatatcctt ttccattaaa tgtaggtaca gaaagtattt tttcatacaa
     2101 cttcgtacta tgtgtggcaa atataactcc tttaacctca ttatctttat aaattggtac
     2161 agaatataca ttaatattac tattggtatt tttatcatct tctattacat cttttaattt
     2221 tcctgttata accacttctc cttgcattga ttttaagaaa taatctcttt tagagaagtc
     2281 ttctatataa ccatctgtag tgtatgctgt accatttggt aaaataactc ccattctttt
     2341 aaatgaacta ttaatagctt gcctttttag tatagaaagg atattatcta cttcaaaaga
     2401 ctcttctcct tcaataaata ttgaaattga ctctagtaac tttatatctc cattaatttg
     2461 tttttttaac acattgacgc tttgatttgc tatttctgaa agatattgat tcgtttcttc
     2521 acctaatgtc ttattcaacc ttgaaccata aatcaaacag gaaattgtaa ttactaagat
     2581 acttatgaca gcaattatgg acaaaccata cctattattt tttgcatcac ttaacattat
     2641 gcttcctgcc tttctcgaat ctatgtatgt ataatcatta tcttatgaca attattgtaa
     2701 acattttata ataatactta tttttccata tttactgtaa ttatacaaaa ttataaactt
     2761 tattacaaca ataaattaat taatatagat attagtgttt acattattgc attatctatt
     2821 ttacaattat ttacatataa aaaaatgtaa tatcaaagtg aagcttgata ttacattttt
     2881 gttcttgtat tttgtttaat ttttatgcta catcttactt aaacactcat ttgcaacttc
     2941 tttatcatca aaatgatgct taactttacc tataatttga taagtttcat gtcccttacc
     3001 tgttataatt acaacatcat ccttttttgc catacttata gcttctgcta tagcttgttc
     3061 tctatcaaca acaacatggt aattttcttt tttcttgtca attccttcaa gtatatcttt
     3121 tattattgcc tctggttctt cagttcttgg attatcagaa gttataatac ataaatcaga
     3181 atatttttgt ccaattgctc ccatcaaagg tctcttttca gtatctctat ctccaccaca
     3241 cccaaacaca gaaattattt ttccttctgc aaattgttga gttgtcttta gtacattttc
     3301 aagtgcgtca ggagtatgag catagtctac aattacgctt atacctttat catttggaac
     3361 tgtttcaaac cttcctgata ctccattaga aagtctaagg ccttctttat atattggttt
     3421 aggtatgcca agtacatagc aagcagctat aactgctaaa gtattatata ctgtaaacat
     3481 tcctggaact ggtataaata ttacttcttc atatgaaggt gttattaatc tataagatac
     3541 tccatccgaa tctgatttta tatctctagc cataaaatca gccttagtat caacaccata
     3601 agtgtaacaa ggtacattta taccttttat attttcatat atcttttttc caccttcatc
     3661 atctatgttt attatatttg ccatagtagt cttaaaaaat aatttttctt ttgcttttct
     3721 ataagcctct aaatctttat gaaaatctaa atggtctggt gttaaatttg taaatatgcc
     3781 tagtttaaag tctgtttcat caactctatt taatgccaat gaatgtgatg aaacttccat
     3841 cgcacagtag tcacatccat tatccaacat attattaaaa tgatattgta aatctatact
     3901 ttctggagtt gttgaattgg aaacaatgtc tttttctcca tcaaaaattt ttatagttcc
     3961 tataagtcca actttattct tatttaaagt taatatctca tttagtatag tagttatact
     4021 agtttttcca ttagtacctg taactcctat aacgttaaat ttttgagatg gatgattgta
     4081 aaaattatca gctacttttg ccatatcttt tcttgtatct tttaccttta taaatgtaca
     4141 tccttttatt gctacatctt cttcaacaag aaatgcactt gctccctttt ctatggcatc
     4201 ttttatatac ttatgaccat ctgacacaaa tccttttata catataaata atgtaccttt
     4261 tgtaaccttt cttgaatcat attgtacatt attaatatct atatttaatt cacccttgac
     4321 attgattata tccaacccct gtataatatc atttaatttc ataaatgctt tcctcccttt
     4381 aaactctttc tgctaaaatt acactacata taatattata ttaagaattt tttactatgt
     4441 gaatggttaa aaatgcttga aacattgcaa aaaatgctct ataattttct ataatataac
     4501 tgaggaaagg tcttctataa aaactatgga taataaaatc gggtacttaa acgataactt
     4561 taaaatattt aatattagag ataaaaaaga tatacaattt gaataccata atcatgattt
     4621 taataagata attatattta taaatggaaa tgtaacttat tttgttgatg gtattcccta
     4681 taaactaaag ccttgggata tactctttat cagcaataat gaaatccata aaccagttat
     4741 agattcaaat gtcttttatg agagaattgc aatatggatt agcccagaat ttatgaaaaa
     4801 aaatagtgat gctagtagtg acttagaaaa atgttttaaa atagctacag agaataaatg
     4861 caatagattg cgtttaaata tgcatgatat tgaatctatt aagagtttta ttgagcaaat
     4921 aaatatttcc gaatccaata ctgaatttgg aaatgaaata ttgagaaatg cattatttat
     4981 acaacttatg atattcttaa acagaatttt cctaaaaaat gaaaatatgg aatcaacaga
     5041 agatgttttt tatgatgaaa ctattaagaa agtattaaac tatataaatt caaatataga
     5101 tgataagctt tcaatagata gcatatcgtc tgaatttttt ataagtaagt attatcttat
     5161 gagaaaattt aaatctcata ctggaacgtc tatacacaat tatatagttc aaaaaagatt
     5221 aattttggca aagtcactaa taaccaatgg tcaccttatg agtgaagttt gtaataaatg
     5281 tggattcaac gattattcta gttttgtaag ggcatttaaa aaaaactacg gtgtatctcc
     5341 taaaaattat ataaacaata aaaatccttt ggatattact cttttagatg aatagataat
     5401 ttatgtaaat tttattttaa taaaacttga agtattagtg tatttatctt actaatacct
     5461 caagtttttt aatgttttaa aatatatcaa aatataatga tatatatttc tattacctaa
     5521 aatatatatt aaataaattg catgttaact gaacctattt atatatttat taaatctcac
     5581 ataactttta gttacactat catatagcta tcctattttt tcaatatcat ctcttaatat
     5641 acttactcta ttctcctctt ttgctgtggt tactcctttt ttaatgctta atttctgatt
     5701 ttgttctaaa ttcgtaaata ctacaggact tattgttgat agaccatttt tttcaatata
     5761 ttctaagtcc attttaacta atttttctcc cgcttttact tcatctccta cattcacaaa
     5821 tacttcaaat ccatttcctt ctaaatttac agtttctatc cctaaatgta taagtatttc
     5881 tactccactt tttttggatt ttattattat tgcatgctta gttggaaata taactccaat
     5941 agttccatca actggtgaat aaatcaatcc atcactagat ttaattgcaa atccatcccc
     6001 catcagttta cttgaaaata cagaatctgg cacttcttct atatctaata tctctccatt
     6061 tgttaatgaa tcaatctttt ctactaaagc attttccata tttgcaactt ccatattttt
     6121 tttcacttta tttttatcaa catttttagt aacagtactt tcaacctttg tttttccctc
     6181 tattatatct tttatatcag actttattga atccgatttt ggtccaaata tagcttgaac
     6241 attgttgcca ataaccataa cacctgctgc tccaagtgat ttcaattttt ctttatctac
     6301 tttatttata tcatttacaa ccaatctaag tcttgttata cacgcatcta ggctctttaa
     6361 gttgtcttta tttcctaaag caatcaaaat ctctgatgct aattcattct tagacatttc
     6421 aatgacttca gcgttagttt ctgactcatc ttctcttcct ggtgtcttta aattaaattt
     6481 tcttatcata aatctaaata cactatagta taatactgca aaaccaatac ctaccaagat
     6541 agccaagtac catggagttc tattaggaag aacaccaaat aataagaaat ctattaatcc
     6601 tcctgaaaat gtgaatccaa ttttaactcc taataattgc ataattgcgt atgataaacc
     6661 tgaaaataaa caatgtattg caaataatac tggtgcaaca aataaaaacg caaactctat
     6721 tggctcagtt attcctgtta aaaaagaggt taatgctgct gatgccatta tacctgctac
     6781 aaattttttc ttttctggct ttgcttcatg caccatagca agagctgctg ctggaagacc
     6841 aaacatcata aatggaaatg cacctgtcat aaaagtacct gctgtgaaat ttactccatc
     6901 tttaagttga gcaaagaata tcttttggtc acccattaca agctcaccag ctttatttac
     6961 atattctcca aattgatacc aaaatggatt atggaaaaca tgatgcaaac caaatggtat
     7021 taatagtctt tccaaagaac cttccataaa tgctgctagt gtttgatttg cttctataac
     7081 attttttgag aaactaagta atccctcttg tattggaggc caaataaaag acaatataat
     7141 tcctattgct atagcaacta atgaagtaac gattggtaca aatcttttac ctgcaaaaaa
     7201 acctagatac tgaggtaatt caattttata aaacttttta taaagataag cagccactat
     7261 ccctataata ataccaccaa atacccctgt ttgaattgtt ggtattccaa caatacttgc
     7321 atacatagaa ttgtctacca tatcttctgt aacacccgcc ataacaccca tagatgtatt
     7381 gagaattaaa aaacctacta tggctgccaa tgctgcaaca ccttctcctt cagttaaacc
     7441 aacagctacc ccaactgcaa atataagtgg taagttatta aatataattt gacctgaact
     7501 ttccattata cttattataa gttgaaatgt aggattcgcc aaagctggaa aatgagccaa
     7561 caatgcgtct cctgctaaag cattactcac gccaagtaat atacctgctg caggaagtaa
     7621 tgcaactggt agcattagag attttcctac tttttgtaaa acaccaaata cttttttcat
     7681 ttacttttct ctccttatta ttttatattt ttttaaataa aaaagactta agtttataaa
     7741 aagttacatt agtatactta attctaaaat taaacttatg ttaatataaa caaacctata
     7801 ttaacctaat ttcagaatta aaaataaact attataattt t
//
LOCUS       DAEORZ010000169         7657 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEORZ010000169 DAEORZ010000000
VERSION     DAEORZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.173"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            5..316
                     /locus_tag="KRH39_003330"
     CDS             5..316
                     /locus_tag="KRH39_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG8513208.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      409..501
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            601..1395
                     /gene="thiD"
                     /locus_tag="KRH39_003331"
     CDS             601..1395
                     /gene="thiD"
                     /locus_tag="KRH39_003331"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBG8513209.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RIGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1419..2207
                     /gene="thiM"
                     /locus_tag="KRH39_003332"
     CDS             1419..2207
                     /gene="thiM"
                     /locus_tag="KRH39_003332"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBG8513210.1"
                     /translation="MYNLIKNVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2230..2862
                     /gene="thiE"
                     /locus_tag="KRH39_003333"
     CDS             2230..2862
                     /gene="thiE"
                     /locus_tag="KRH39_003333"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG8513211.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISTILKAEDISKEVEKWTLKI"
     gene            3001..3303
                     /locus_tag="KRH39_003334"
     CDS             3001..3303
                     /locus_tag="KRH39_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8513212.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3369..4118)
                     /locus_tag="KRH39_003335"
     CDS             complement(3369..4118)
                     /locus_tag="KRH39_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513213.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4112..5074)
                     /locus_tag="KRH39_003336"
     CDS             complement(4112..5074)
                     /locus_tag="KRH39_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513214.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5078..5536)
                     /locus_tag="KRH39_003337"
     CDS             complement(5078..5536)
                     /locus_tag="KRH39_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG8513215.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5892..6248
                     /locus_tag="KRH39_003338"
     CDS             5892..6248
                     /locus_tag="KRH39_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8513216.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6320..7036
                     /locus_tag="KRH39_003339"
     CDS             6320..7036
                     /locus_tag="KRH39_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513217.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7029..>7657
                     /locus_tag="KRH39_003340"
     CDS             7029..>7657
                     /locus_tag="KRH39_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513218.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYVFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVI"
BASE COUNT         2979 a          890 c         1255 g         2533 t
ORIGIN      
        1 taaggtgtat tttacatata ttataagatg taaagatgat tctctataca ctggatatac
       61 cagtaatata gttagaagaa tgaatgaaca taaattagga attaattcaa aatacacaag
      121 agcaaaggga tttgaaaaac tagaagttta ttttgtgacc aacacaaaaa gcaatgctat
      181 gaagttagaa tactatataa aaaaattaac tagaaataaa aaattgtcta taataaaaaa
      241 cccaagtata cttataaatt taattgataa taaagaagat tatattattg gtaaggaaat
      301 agaacaatta acataaatat ttatagtaac attataatgg gtgcatatgg tgtaaaaact
      361 tgacaataaa tactattgta atataatgaa gttataaata tagtaaatta aaatagctag
      421 gggagccaag agctgagagg tataccgacc cttacacctg atctggataa taccagcgta
      481 ggaaagctta gtaaaaattc aagtatgtat tatttgtgat atatgctatt tctaagctat
      541 acctattgta ggtatagctt ttttaattaa gttatttaaa ttaaacttta ggaggcaaca
      601 atgagtaatt ataaaatacc aacacttaca atagctggat ctgattcatc tggaggagct
      661 gggattcagg ctgatttgaa aacgtttagt gcaattggta cttatggtat gagtgtaata
      721 acagctataa cagcacagaa tactaaagga gtttttgctg tagaggattt aaataagaaa
      781 ataataaaaa aacaaataga ggcagtcttt gaagatatac ctccaagagc tgtaaaaata
      841 ggaatggttt caagtcctga gattatactt gaaatagttg agaatctaaa gaaatacaat
      901 ccaaaatatt tggttgtgga cccagtaatg atatctaaaa gtggatatta tctattaaag
      961 ccagaggcaa aagaaaactt aattaagtat ttgataccac ttgcatacat aattacacca
     1021 aatataccag aggcagaaga aataacagga ataaaaatac ataatgtaga tgatatgaag
     1081 agaataggag aagaaatact acaacttgga cctaaatttg tacttatgaa aggaggtcat
     1141 ttagatggag aagcagtgga tattttagtt ggtaaaaata tatttaaagt ttataaaagt
     1201 gaaagaattg ataaaaaaaa tacacatgga acagggtgca cattatcttc tgcaataaca
     1261 tcttatctag cattaggata tgaaataaca gaagcagtaa atctaagtaa aatctatata
     1321 acagaagcaa taaaaagaag ttttgatata ggtcatggag taggaccagt acatcatttt
     1381 tataaatttg agtaataaaa aaatattgga ggtagaatat gtataattta attaaaaatg
     1441 taaaaaaatt aaatccattg gtaattcatt atactaataa tgtaactata aatgattgtg
     1501 ctaatgtaac tttagctgta ggagcaagtc ctcttatgag tttttcatat gaggaagtag
     1561 aagaaatggt aagtgtagca aactctgtag taataaatat aggaactatg aactcaaata
     1621 tgttggattt attcctatta gctggaaaag cagcaaataa gtacaataaa cctgttgttt
     1681 tggacccagt tggagtattt gcaagcaaag cgagagcaga acttacaagt agacttttaa
     1741 atgaagtcaa atttagtgtt gttaaaggga atgtatcaga aataaagttt ataggtggct
     1801 ttaatgtaag gggtaaaggt gtagattcat ttgatgaaga agaagattct actgagataa
     1861 taagaaagat tgctgaaaag cttgaatgtg tagttgtagc aactggaaaa atagatataa
     1921 taactaatgg taaaggtaca tataaaataa acaatggaac tgataaatta aaaggaatta
     1981 caggaactgg atgtatgact gctagcttga ttgcaagttt tatggctgtt actgagaata
     2041 tattagaagc agcaacaatg ggtgttctta caatgtcttt aagtggggaa ttagctaatt
     2101 taaataatcc accaataggt acatttaaag aaaatcttat gaatgctatt tatcaaatgg
     2161 atatagatac tttaagtaaa aattctaata ttgaattttt aaattaataa agagtaaggg
     2221 agacttaaaa tgattgataa agaatcttta aaaaaatgtt taaagttgta tttagtaact
     2281 gactctgaaa tgcttaaagg tagagacttt tataaatgtc ttgaagatgc aatatcttct
     2341 ggaataacaa ctgtacaact tagagaaaaa aatgcttctg gaagagagtt tttaagaaaa
     2401 gctatgaaac ttagagaaat aactaaaaga tatggtgtta agtttataat aaatgataga
     2461 gtggatatag cacttatttg tgatgcagat ggagtacatg taggacagag tgacattgat
     2521 gttagagagg ttagaaagct tataggaaac aataaaattc taggagtatc agcaagaact
     2581 ttagaagaag ctatttgtgc aaaaaatgat ggcgcagact atttaggtgt agggtctata
     2641 ttcacaacat ctactaaatt agatgcaaaa tcagcttcgt ttgaaactgt taaagaaatt
     2701 aaagaaaaag tagacatgcc atttgtacta attggaggga taaatttaga caatatagat
     2761 aaacttaaat gtttagaaag tgacgggtat gctataatat caactatatt aaaagctgaa
     2821 gatatctcta aagaagtgga aaagtggaca ttaaaaatat aaagttggct ctggtagtga
     2881 aaaaaatgga aaaatttgat ataatctaag agtaaaattt aagcaattat aaatgataaa
     2941 cttttctaca tatacttttt aatcaagata tatgtagatt tggttaaaaa ggagctataa
     3001 atgattagga tattgttagt gtgtgttgga ggcatgtctt ctaccctatt ggtaaataaa
     3061 atggagaagg atgcaaaaaa aagaaatata gactgtaaaa tatgggcagt tggagaagga
     3121 gatataaaat cggagcttga taactttgat attttacttc ttggaccaca acttagattt
     3181 atgcttgatg acgtaaagtc tatagttgga gatagagctc cagtatctat aatagatatg
     3241 gtaaactatg gcacttgtaa tggtcatgct gtgttaaatt cggttttaga aatattaaaa
     3301 taaataataa aagtggattc tatataatag agtccatttt tattatttgt tattaatagg
     3361 cttaaaactt agcttaccat ttcttttttt aatctccaca tagcaagtga aaaacttgaa
     3421 cagcacaata ttaaaaatag taggataaca ggcagtatat ctccaataag aatagtctca
     3481 ccaaatatta agctacgtaa agcgttaata acatgggtaa atggattgag catcactgct
     3541 acttttaatc ctccagataa actttctata gggaaaagag ctgaacttaa aaagaaaata
     3601 ggaagtacaa ttgcagtcat aattgtttca taaactattt catttggcaa taataaactt
     3661 atagaataag ctagacttga taaaaagaag gctgtcataa atatcagtac tatcataagt
     3721 aggattccac caatgccaga ttctatcctt actgagaaga atatacttac tatgcacaaa
     3781 atagttactt ctatgaaaga aactaagata gcttctaata attgaccaag gacgattgaa
     3841 tacctactga taggtgcaat taagactcta tagaaacttc cactgttctt cattataaag
     3901 tttataatac caccactact acaagaagaa aacacaacca gtacaatgat acctggtagt
     3961 ataaaagcag tataattatt gatatttata ttattcatag tttggtttgc aatagaacta
     4021 taaagcacaa gccaaagaaa aggttgtaat atagtaacta caaatgagat tggattttta
     4081 aaacgccact tcatactacg ccataagata tttaacattc ccattcctcc tttaagcttt
     4141 ttttagaact tgtaagtgct aaaaatacat cttcgagact tggctctaca atttctattg
     4201 catcaaattc tatatcatgt tctaaaagcc atttatttat taaagtaaaa gcagttctac
     4261 tatcgtttac acttataaaa attgaatttt ctcgtacgct cataaacttt actaaaccag
     4321 tacttttaat tgaatcttta tattttttta tatcttcagt attatggaaa gttatacgta
     4381 gtatattttg tctaatgtaa ctacgtaaac ttgatggtgt tccttgtgct aaatcctttc
     4441 cattcttcat aatacaaata ttatcactca attgttcagc ttcttcaaga taatgagtag
     4501 ttagaaatat agttgtgcca tactcatctc taatttttaa taacatatcc cacatagatt
     4561 ttcttgaatc gacatccata ccaacagttg gttcatctaa aaataggatt ttaggagatg
     4621 ataccatatt catagctatg tccaatctac gttttacacc ccctgaatat gatgaagtag
     4681 gatattttaa gtaactagat aagtcaaact tatcaattaa agagtctatt ctttgttttg
     4741 caatctgtgg ttctacttta tacattttac tttgaaatac catattttcc ataagggata
     4801 agtgttcatc aatagagatt tgctgtgcta cacaagcaat ttgagtacgt atccaagaag
     4861 gattatctac taaatctttt ccaaacattg ttacatttcc agatgtaggt ttgtaaaagg
     4921 tagtcaaaat atttataaga gaagatttac cagcaccatt aggaccaagt aaagaaaaaa
     4981 tttctccagc atttacatta aagcttagac catttaaagc tttcacacca tttttatatt
     5041 cttttactaa attgtctatt tcaatcgcta acatatatta ttcctcctct ttaagtggaa
     5101 atattatctc tgtaatatag ttatctgggt ttccttttat caacatacct ggtcctttaa
     5161 taaatacttc tctccaagga gtttgtattt ttatattatt ttcatgaata tatttaaata
     5221 tcatttcata cgtttttggc aaattactat aagagcctgt gtgagtaaaa aataaagctt
     5281 ttgttttagg aaattctttt gtagttatac ccatacgatt tggagtttca gcagttggaa
     5341 cacataattc catttctgca acaccagttt tttggtcaac atcatagtag caaaagaatg
     5401 gtgcaccgtt agactttcct tttatagctt taaatacatt tggaaaatac tttgttgcct
     5461 cagtcatagg tccattataa cgcatggtag ccactgtgac tgattcgata tttttgattt
     5521 caattttgca attcatttgt ttacctccta atgtgtgttc atattcttac tagattttta
     5581 agcattaata catataagat tttgattagt tgaaaacata taatcttatc tcgtttgctt
     5641 gttttcaagt ctaacataac ataaaatata tatcctctca tttgatgctc cattttgtaa
     5701 agttattatt taagctttac tttagatttt ttagcaaatt aaaataaagc aatttataaa
     5761 ataagtattt acaaattaag tgtattaatc gtataataca cttaatagga tactagataa
     5821 agtctgaaat aaaggcgtat ttttttaaag taagtgtact atagtattaa tacactagaa
     5881 taggaggaat catgaatttt gaattagata atacaactcc catttatttg caaatagtaa
     5941 agtatataaa gagacaaata gtaacaggag agttgaaacc aggagagaca ataccatcaa
     6001 gaagagaaat ggcacttaat ctaaaagtaa acttaaatac agttcaaaga gcatataagg
     6061 agatgggtga tatgaatata ataaatactt ttaaaaatta tcaaagcagt gtaacagttg
     6121 atgaaaatat attaaaaaat ttaaaattag agcttataaa tgaatcacta tctgttttta
     6181 tagaagatat gaaagcaata aatgttagca aagaagaagt tttaaaaata atagaagata
     6241 agtattagtt ttttaacaaa tagaaaactt agtttagaaa gtttatttat aagcatattg
     6301 gatgtggagg atagtaaata tgatagaaat aaagaatgtc agcaaaacat ataaaaggat
     6361 gcaagggctt aaaataagaa agatagaagc tttaaaaaat gtaagtttta atatagaaaa
     6421 gggaaaaata actgcattac ttggtataaa tggtgttggt aaatcaacta tgttaaaagc
     6481 aatagcagga cttataaaca ttgatagtgg agaaattcgt atagatggag aaaaaataaa
     6541 tgaaaaggta tacaataaat tggcttttgt tccagatgta caatctcact ttagcaatac
     6601 aacaataaaa gaaacatttg aatttatgga aattttttat tcaaagtgga ataaagaaaa
     6661 atcaaaagaa atgatggaca tatttaaatt ggatgaagat gaaataattg ataacttgtc
     6721 aaaaggaaac attgcaagag taaaattaat acttggattt tgccaagacc cagagtacat
     6781 acttcttgat gaaccattta caggaattga tttgtttaag agggaagagt ttataggagt
     6841 aatagcgcaa tatatggagg aaaatcaagc cattatcata actactcatg agatagtaga
     6901 gatagaaagt ttagttgatg aggttgtaat tcttgatgaa gggcagatta taacttcatt
     6961 taatgcagaa gaattaagag aaagagaagg taaatctata ttagataaga tgagggaggt
     7021 atataagaat gaataaactt ctatatctaa taaatgtaga tttgagaaga agcaataagt
     7081 tttatatagc ttatatatca tttttttcat taattacact tagtttaaat ttatttgaaa
     7141 ttaataaatt taaaaattct aaatttatta tgaacttagc aatagaagat tttggaggta
     7201 tattttatgg actagggata cttagtaatc ttggttttat ccaaagtata ttgttctttg
     7261 ggatattggg actttttata tactatgttt ttatgtggaa aagagagttc ttttcaaata
     7321 atcaaagtat ttatacactt atgatgttac ctcaaaataa gtttaagatt tgtatatcta
     7381 aatcaatagc cttgataaca atgatatatg gttttttgat aagtcagaca gcaacattat
     7441 ttatatgtaa atatatattt aatttcattt ttagaaatat gcctataatc aatatgaact
     7501 ttgccaaaga tttatattat ttaaacttga agagtatacc aattgatttt atctctttta
     7561 tggctattta tgtattttta ctacttatag ctatttctat tgtaaattgc tgtatattat
     7621 ttatcttttc ctatataaat aaattttatg taatttt
//
LOCUS       DAEORZ010000170         7598 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEORZ010000170 DAEORZ010000000
VERSION     DAEORZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.174"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..164)
                     /locus_tag="KRH39_003341"
     CDS             complement(<1..164)
                     /locus_tag="KRH39_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513219.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYS"
     gene            complement(232..645)
                     /locus_tag="KRH39_003342"
     CDS             complement(232..645)
                     /locus_tag="KRH39_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513220.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEENLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLISVFFSANYTRGLD
                     ISNLDIGIILLAILFIQSLSKLILYIHFDRKGICN"
     gene            complement(1479..2108)
                     /locus_tag="KRH39_003343"
     CDS             complement(1479..2108)
                     /locus_tag="KRH39_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBG8513221.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDVRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(2187..3149)
                     /locus_tag="KRH39_003344"
     CDS             complement(2187..3149)
                     /locus_tag="KRH39_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG8513222.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQNQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(3911..7430)
                     /locus_tag="KRH39_003345"
                     /pseudo
     CDS             complement(3911..7430)
                     /locus_tag="KRH39_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2409 a         1416 c          816 g         2957 t
ORIGIN      
        1 attgaataac atccaaaaac tattaaccaa ctcaaccctg gaagagaagc agtgcttgga
       61 cctttaaaaa tatacttagt ggctaataca gctataaaaa aataagttat tacaagcaac
      121 tccaggataa gctttgatct tacaccagtt tctgatttag acatataaaa tcactcctta
      181 aaaattatat tctctatact ctttaatttt cgtttttatc aatattatca cttagttgca
      241 tattcccttt ctatcaaaat gtatatataa aattaattta gataaagatt gtataaataa
      301 aatagctaag agaattatac ctatatccaa atttgatata tccaaacctc ttgtataatt
      361 tgctgaaaag aaaacagata ttagcaaaat agaatataac attatttttg atgcaatatg
      421 atcaaccttt aataacataa gttttgacgc ctcgtcaact aaatcattat attttttatt
      481 atacatgaag tcacatgccc aaaaagcgaa taaaattata aaagtaatac aaagattttc
      541 ctctttaaaa ataaactttt ttaataatga gtctataaaa aaataggata tcataagtaa
      601 ctctgaaaca agatttgttt taacaccaag ctctgattta gacatataaa atcacccctt
      661 aaaagttata tatttataac accttatgtt ataaatatat aacttttcca aataaattgc
      721 aatagaatat tgaaaatatt caataatatc ttcataaata tattttataa aaggttttat
      781 aataaagatt acaataaagt actaaattag tttatttcat ctattatctc aatcatatca
      841 tttactaact attattctaa tgagcatata acattgaaat ttataattat acaacctaaa
      901 tgcctcgcaa tgtaccctca aaaacatcat ttcctgtaac tgaagtataa ttggtatatt
      961 ttatcactga aataaaaaaa cactagtcaa taaagactag tattttcaag tattttatca
     1021 ttcttttttt aattattgga gacgaatcat cagaataatg cccataattt atctatatgc
     1081 aaattctaaa tttaaaataa agtttcagat aaatttacta ttctatgcta tgatacaaca
     1141 gaaaaaactg aaatctatga gtggtagctt catataatcc tagttcctac agtaatcaaa
     1201 actgaggttt attctatatg taactaatga taaattttat tataggagtt attagctcta
     1261 tttgtgttac tataagtagt aattatatat atgataattt tttaacactt ttctgttggc
     1321 tatatagaaa gtggtttaga atttaaattt aaataaaatt taaattcacc aaaaagaaat
     1381 ctatattaga aaccactact ctattccacc agattgtaat tttttgttct ttatatcaca
     1441 atgctatacc atattaaatt taaaaataaa ttttaaattt atttgttaca attcattaat
     1501 tcaaaaaaat attttgagtc atgagttttg taaagattta gccatgaatt taatgcttct
     1561 gattcaaaaa catctaaaag tttcaatact tgaaacgtaa attccaacgg agcgattcct
     1621 gaagccgtaa ctaaattttc atcactaact acagattccg ttttataata tttttctcca
     1681 gagtaatttg gtattaccat tttaagaaac tctaagtcat tacttgtatg atttctaaca
     1741 tctaatagcc cttttttagc taggcctaat gtagctccac atatggctgc aactatagaa
     1801 tcttgtatta aagcttcttt aactttttct aatattatct catgattcat ggataaccaa
     1861 gtatttcccc ctggcaaaat taataaatcc ttactttcca tattaaactc atcaactgta
     1921 atttcaggta aaacttttaa cccacccatt gttataatag gatttttatc aattcctaca
     1981 gtaattactt ttagtggttt taatcctttt ttaaaatatc ttcctgtatt taattcagca
     2041 gttaaatatg ccacttccca atctgacata gtatcaaata catacatata tacttttcta
     2101 tttttcatct tttttactcc ttatgtaatt aatttatata tctaattata aaataactta
     2161 actgacatat tcagtcagtt aagctattaa atttgataat aatcaattaa ttctagtaaa
     2221 tcttcaatta ttttcttcct aaggcttagt ggttcaatta cttgtattgt ctttctataa
     2281 ggtaaaagta tatttggtat gtacttgcat attacttcct cttcaagcat aaatattatt
     2341 ttattaggtg ttctttcttt caagtagtgc cctaaaaacc aatgttggct taaactatct
     2401 aaagaatcta tactcccttc tacaactaat tgaattatat tttgttcatc ttttatttca
     2461 ggtataattc cttttataaa aaattcactt gctgaaaatt ctgtcggtct gttaaatgta
     2521 tttgatgttt ctatgatttc actaatccta tccactctga aactacgtac ctcatttctc
     2581 atattacaaa atgcaactac gtaccaatta ttattccagt gaacaattcc atatggattt
     2641 ataattctat attttgattt tgctttacga cctgcatggt agttgatttc tacagatagc
     2701 tcctttaata tactattctc caatttttta agtactgatt ctatagacac tttcccaatt
     2761 ggttttatta cttcaagggc tcttaagtgg tttgttaaaa tatcttcttg gttttgattt
     2821 gaatacattt ttaactttaa tatcgattta tctaatgctt cgtctaagaa gtatccttct
     2881 tttttagcaa atagagctgc atgaagtaga gatttttgct cttctatatc aaaaaaaaga
     2941 ggtgctttag taaaattatt taaaaggcta tacccaccat tatgacctgt atctgaaatt
     3001 ataggtactc cactcgcaga aagtacatca atatatcggt acactgttct tatatttatt
     3061 tctaattttt cagctatctg ttttgctgta atttttttgt cagagtttaa catccacagt
     3121 atagacatca tattgtcaat tttagccatg tagtttcacc tctttctatt tgttatataa
     3181 agttattata taactttttg attgattttt tacaaaaaaa cattagtaaa ctgctaataa
     3241 tttatactaa gatttatttc aaatatttat agatagtctt tttctatagt taagttatcc
     3301 attaaattac atagtagtat atccatttta gttgatatat tttcagctac ctcccttgaa
     3361 tattattaca ataagtctaa atcttctttc ttccataaat atattatgag gaaagactat
     3421 catctttaat ggtttaatag tataaaagat attaatttaa aaaaatctat ttttatatca
     3481 gatggatatt gttttttata aaactgaaaa aatcacctaa tagaatctat gattttttaa
     3541 cataacctcc ataaattagt accaattatc acttcaaatt caaattttta tctgaagaat
     3601 atacaatcat atttccatac tcaacataat attgagtgtt tggcttgtat ttacaaatta
     3661 ttattctaac aaaaacaact atacatagat taaaatcatc ttctaaacat aactactaaa
     3721 attgaattaa tgatatcaat tccattaatc tcttttaata aaaaattctc ttttctatat
     3781 gaaaattatt tcaaaacttt taagttaaat acaaaaatga ctataaatag atttttattt
     3841 acacaaatct atttatagtc actatagttt atccaataaa ataataaaat aaatatgtac
     3901 acaataaatg ttatttacgt cttaagtatt tatttgttag atttttaatt aaaaaaaaca
     3961 agctaatgaa cattaatgct aaatctagca ttatattgct ttgatctcct gtttgtacat
     4021 tttgacctac attactatct aaactttcat ctgtatctat atttatatcc ggttttccat
     4081 ctccatctat atctatattt atatctggtt ttccatctcc atctgtatct atatttatat
     4141 ctggttttcc atctccatct gtgtctatat ttatatctgg ttttccatct ccatctgtgt
     4201 ctatatttat attcggtttt ccatccccat ctgtatctat atctggtttg ctatttcctc
     4261 cattatctgg gtctggttta ctatttccac cgttatctgg atccggtttg ctatttcctc
     4321 cattatctgg atctggttta ccattgccac cgttatctgg atctggttta ccattgccac
     4381 cgttatctgg atccggtttg ctatttcctc cattacctga atccggtttg ctatttcctc
     4441 cattacctga atctggtttg ctatttcctc cattatctgg atccggcttg ctatttcctc
     4501 cattatctgg atctggctta tttagatttg gattacctgt atcataacct cctgagccag
     4561 tatctacatt tttatcaggc ttccaataat catctactcc atcattatct gtatctattg
     4621 ctggacgatt ataatctgta tcagggtttc catctccatt ggtatctata tttttatcag
     4681 gtttccaaat accatctcca tttccacctt ctgtactagg cttccattca cctgtattat
     4741 cagtatctat gttaatatct ggcttccaat caccactcgt atctatattg atatctggcc
     4801 ttccatcacc atcagtgtct atatttatat ctggtattcc atccccatct acatctacat
     4861 ttgtcatatc taactttaac ttcttttcat cctcactacc ttcaatgtaa tttatatcat
     4921 ctccaatata ttttacagta atagtttgtt cagtagtaaa aatatgatga ttgctatcta
     4981 aaggtctcca ttcaatagat gcagtaactc tcactaccct tattatgagt ttttaattct
     5041 actggatctt tatattcctt tccattaata tagaattgta cgtaacctct tggagaggca
     5101 caatctggtc cttctttctt atctggtgaa atagtaacat ttaatgtaaa cgtatctgtt
     5161 ggtttaaaaa catcagtttt agctttagac tcttctacaa ctaatgaagt ttctgtatca
     5221 gcaggaataa tttctgcctt ataatatgct ctatgccccc aataagcatc cttaaaagga
     5281 tatatactcg aaaactgtgt tgatgtaatg cttaattgat atttcccagc atcaacttgt
     5341 tttgtaagat catcacttcc taaatttata tctggttcca tcgtttcctc atttaaacgt
     5401 tgagaactaa tagtcataaa ttttttatct gtgagttttt cacctttagg agtagttatt
     5461 ggaataccac catcttttaa tagccaatct aaaaatgatt tttctacgtc ttcttcaaat
     5521 ggtaatccat catatctttt aataatcaaa tatggtggag tatcatcaat agtaaattct
     5581 tcatattgct ttaaaaattg agaacctgaa cctatatctt taatataaaa atcatctggt
     5641 tttagaactt ctccagtaat tttatctata acaactaaat taacagatac ttcagatcca
     5701 gtaaatttat cagagaacca taagtaaaga atcccttgag catctgtata actaggtgct
     5761 ccataaggat aaatttttcc atcaattagc attttaaaat catcaactag tgtagtacta
     5821 actttatctt gccctaaata cgttagagat attttattca taaaaacttt tgttttcttt
     5881 tctaaatcac cgtatgcaac tgctccacct aaacttttga atgttgttgc ttttaatgaa
     5941 ccacctgtta ctataacatc tcccgttgta gcacttaggt caaaaatcca atctcccact
     6001 cctgtcatat aaggtaaaat cgttcctcca gttacaagta tacgatatcc tctatttgtt
     6061 cctggattaa cacatccatc cccaaatgca ctaccatgtg cacctccata tgaataggaa
     6121 agtccaccat taattgtaat attcccacta taaccagaac ctgtgctagg atctactact
     6181 gagggactta atgcattctt tgttgttgta tagtatcctc cacctattcc agctccatgg
     6241 tatgcacctt tagcagttat agtaccacca ttgattgtga tatctccacc actaccatta
     6301 ccgcctccac ctattccagc tccatatcct gtattacgac tcgattcttg acctatagca
     6361 gttatagcac caccattaat aataatattt cctccacttt cagtatttcc accgccaata
     6421 cctgcagaat aataatttcc aacaactttt aaacttcctc tattattact atctagtagt
     6481 gtaaggcgat ctcctttttt taacttgtct ccattttcta gagttacatc tctaggaatt
     6541 tttccaccct caggaactat aaactcgcct tcaatatctc tatttacaac agaatcatca
     6601 ataattagag tagtaccttt tcctgctctc atacctggat aatatacatc acctgactct
     6661 agcttgtttt cactaccatc ttgtaatata acatgtgcat atgcaccacc atctttatct
     6721 ggtgttaaag taaatgggct attagatgca gttttaatat ttactccatc aaatattaat
     6781 tcagctttta catctttagg tataacaatc ctatgtgttg ttggagtatt tggattatta
     6841 ttttgaattg tcatttgttt tccactaaga atagttaaaa cgccacttgc aaatttaaaa
     6901 tcgacattta tttctccacc actaatttta aagtctccta cagatgtagg atcttctgca
     6961 gtggcttctt ccacaactaa tgcattcata tctccttctg catttcctgc attagaatct
     7021 tcttctgcat ttcctgcatt agcatctcct tctgcatttc ctgcattagc atcttcttct
     7081 gcatttcctg cattagcatc ttcttctgca tttcctgcat tagcatctcc ttctgcattt
     7141 cctgcattag tatctcctcc tgcatttcct gcattagcat ccccttctgc atttcctgca
     7201 ttagcatccc cttctgcatt tcctgcatta gcatcccctc ctgcatttcc tgcattagca
     7261 tcccctcctg catttcctgc attagtatct ccttctgcat ttcctgcatt agtactttct
     7321 tctatagtat ctgcgaaagt atttgacgca ttaaatgatg ctactgatcc aagtcccatt
     7381 gagatactta aaatcattga tagtgctttc tttttacatt tttgttgcat aaattccccc
     7441 tccccttaat aatttttaat atacactgta tacatcctta tatatactct aataattctt
     7501 aatatacact gtatacatcc ttatatacac tctaataatt tttaatatat acttaaaact
     7561 tatatttgtc aatgaatata cagttttttt caacctta
//
LOCUS       DAEORZ010000171         7461 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEORZ010000171 DAEORZ010000000
VERSION     DAEORZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.175"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            8..868
                     /locus_tag="KRH39_003346"
     CDS             8..868
                     /locus_tag="KRH39_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG8513223.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            1324..1890
                     /locus_tag="KRH39_003347"
     CDS             1324..1890
                     /locus_tag="KRH39_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG8513224.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            1930..2511
                     /locus_tag="KRH39_003348"
     CDS             1930..2511
                     /locus_tag="KRH39_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG8513225.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(2568..3485)
                     /locus_tag="KRH39_003349"
     CDS             complement(2568..3485)
                     /locus_tag="KRH39_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8513226.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            3615..5246
                     /locus_tag="KRH39_003350"
     CDS             3615..5246
                     /locus_tag="KRH39_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG8513227.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            5249..6196
                     /locus_tag="KRH39_003351"
     CDS             5249..6196
                     /locus_tag="KRH39_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBG8513228.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(6481..6744)
                     /locus_tag="KRH39_003352"
     CDS             complement(6481..6744)
                     /locus_tag="KRH39_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBG8513229.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(6843..7088)
                     /locus_tag="KRH39_003353"
     CDS             complement(6843..7088)
                     /locus_tag="KRH39_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513230.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         2701 a          970 c         1163 g         2627 t
ORIGIN      
        1 aggagtgatg ttattgaaaa aagatagaat aactttttca gaaattttaa tattaattgt
       61 aggctgtata ttaatggcca tttctcttaa cttatttttt aatccacatg caatcgctgc
      121 tggtggtata acaggacttg gagttgtttt aaattcatta ttcggtgtag aactttggat
      181 tgtcaattta cttttaaatg ttccattatt catatttgct tacaaaatac tttcaaaaaa
      241 agattgtttt aaaacggttt taggaataat ttttcttacc atagcactta agcttactgc
      301 aaatatggct acattagata taacaaatga tatgtatctt gcaattatat ctggttctat
      361 tttaatggga gttggtcaag gacttatttt tagaataaat ggctctacag gtggaactga
      421 tttaatggca ctcctactaa ataaatattt tcctacattt agtatacctg ttttaatggg
      481 tattgtagac tgcgttgtag ttgttttatc aggtatagta aatagacagg ttgaaattgc
      541 actttattca actgtggctt tatacatttt agtaaaagtt agtgacttgc ttattgaagg
      601 atttaattat tccaaatcat tcactattat atctgacctt tcaaaagata taagtaaaaa
      661 aataatggaa gatttagata gaggtgcaac tattctaaaa ggagaaggtg catatactgg
      721 agaaaataaa aatgtacttt tagtagtagt tgaaaagaaa gaagttgtcg aattgaaaaa
      781 attagtaaaa aatgtcgacc ctaatgcttt tattataatt acagatatac atgaagcttt
      841 aggtaatgga tttaaaaaaa tagaataact aacaaataaa tagcaagtat aattaacata
      901 tacaaattca actttttata tgttatatta tacttgctat ttatatatta tacttgctat
      961 ttatatatta taattgttgt ttttaagata aaaattaaat gtatcaatta ttgttttata
     1021 caagtatttc caaatccgtt ctcatttaga tagtatgctt agaaatttta gaattattga
     1081 gtctaacttt attacttact ttactttata acaatcaaat acaaatttaa atattataaa
     1141 actacaaaat aatttaaagc atataatgtc aatatgttta aattataaaa tattcatgta
     1201 gttatataat aaataattta taaatatagc caacttagcc taaatacgta tataatatag
     1261 ctaatatggt tcttatactt gataccatca ataaaactaa aaatataaaa aggagaactt
     1321 aaaatgagtg ttagtttaca aaaaggacaa agagttagct tagtaaaaga taataatcct
     1381 gtaaaaaatc tagtagtagg cttaggatgg gatatgaata aattgggtaa aaaaaattat
     1441 gacctagatg catttgcaat agcattgact aaccaagata aaatgaatgt tagaggtgac
     1501 ttggtttatt ttggaaatct taaacaccct tctaaagcta ttatacacac tggagataac
     1561 ttaactggaa aaggtgaagg tgatgatgaa caaataattg ttaatttaga agacatacct
     1621 gaatatgtac ataaaatagt ttttggagtt tctatataca aagctaaaaa aagagaccag
     1681 gattttggtc aaatgaacaa ctcttttata agattaattg attcaaattc caagcaagaa
     1741 ttatttaaat ataatcttca agaagacttt tctggtaaag taacagtttt ggcaggagaa
     1801 atttatcgta gaaatgaaga atggaaattt aatgctttag gaattggtca aaatgaagag
     1861 cttcgtgaat taattaatac atataagtaa taattacaat aaaaaattta taatgaggag
     1921 ttggtttaaa tgggtataac tttagcaaaa ggacaaaaag taagtttaac aaaaagtaac
     1981 cctggtctaa aaaaagtaat agtaggacta ggatgggata ttaataaata tgatggtggc
     2041 tttgatttcg atttagatgc ctctgcattt ttaactggaa ctgatggaaa agtaacgaat
     2101 gatggagact ttatattcta taataattta aaacacgctt ctggagctgt tgaatatatg
     2161 ggtgataata gaacaggtgt tggtgatggt gatgatgaac aaataaatgt agacttatct
     2221 aaaatacctc aaaatattgc taaaatatca tttagtgtta ctataaatga agcaattaca
     2281 agaagacaaa atttcggaca agttgaaaat tcatatataa gaatatataa tgaagaaact
     2341 aatgaggaac ttattaaata tgaattgggt gaagatttta gcatagaaac agcaatagtt
     2401 gtagcagaac tatatagaca taatggagag tggaaattta atgctttagg ttctggattt
     2461 gaggatggac ttgcaggatt gtgtaaaaat tttggcgtta atataggcta atatgtctaa
     2521 ataaaaataa gagtatattg atatactctt atttttattt agatatatta taaactgttt
     2581 acacttctac tcttctttct cgcttgtttt ttcccatatt ctactgaaat atcaaaaaat
     2641 gctgaaatat tttctgtaaa aaacttgctt ctatgatatg caatatcaaa tgtcctatgt
     2701 aaattaatac cttcaatatc acaagcccac aaaagatgct tttttaaata atcctcaata
     2761 agtaattcgg atataactgc aatgccaaaa ttatctacaa ctgctcttaa tatagcttca
     2821 gagttataac atgaccatct aatattcatt gggattttta attcttttaa ttgcttttct
     2881 aattgtgctc ttgtaccact tcctaattca cgcataatta atggttgatt tgaaagttct
     2941 gaaactttta tagagtctct tttataaaac tcatgcttgt gtgaacatat aagtacaaga
     3001 ttatcattaa taatgctttt tgtaacaata tctgggtgct taatttttcc ttctacaatc
     3061 gcaatatcta attcactatt aagtaatttt tttgcaatca gtcttgtatc ttctacaaat
     3121 acttcaggtt ctataagagg atttaccttg tataattcta acattatcgg gcttattaca
     3181 catgtaccaa cagttactgt tgccccaact cttatacgga tattttttgc acaatgcttc
     3241 atacaatcat tcatttcttc atttacagct aaaagatgcc tcccatatcc aagaagtttt
     3301 ttccctgcct cagttaaata taattttttt gaaattctat caaatagctt aacaccatac
     3361 tctttttcta tttctgaaat agcttggcta actgatgact gggtaatata catatttctt
     3421 gctacttcac tcattttacc acattcagca accttaacaa agatttcaag actacgaatt
     3481 gtcatattat cacaacctta gcaaacatta gtaaatgctt atgattttat taatatttta
     3541 gtattttaca aatatattgt caagaagtat aattttatta tagagttata aagtagattg
     3601 gagtgtgata tattgtgaaa gtattaatta taggtggagt tgctgctgga acaaaaactg
     3661 ctgcaaaaat aaaacgtgaa atgggtgata attgctctgt tacaatacta aataaaggtg
     3721 atgatatttc ttatgcaggt tgtggtcttc catattacgt tggtaagata attgaagata
     3781 aatcatcttt aatcgtaaat acacctgaaa gtttttctaa actaacagga gcagaagttc
     3841 gttgtggagt tgaagttaca tctattgata gaaatgcaaa acaagtaact ttgaaaacgc
     3901 tttcaacagg agaaaaagaa aatctaagct atgacaaact tgtaattgct acaggggcag
     3961 acccaataaa accaccaata cctggaatag atttacctgg agtattcttt atgcgtactc
     4021 caaatgatgc tataaaacta agagattcta ttgaaaacgg tattaagaga gctgttgtta
     4081 ttggtggagg tttcattggt cttgaaattg ctgaaaatct ttctgcaatg ggtatacgtg
     4141 taagtgttgt tgatatggct gaacatgtta tgcctggatt tgatactgat tttgctgaat
     4201 acgttgaaaa tcatatggct gataaaggca taatgatttt tacaggtgac caggttgtag
     4261 gtattgaagg tgaaaataaa gttgaaaaac ttcgtactaa aaatagagct attaaaacag
     4321 atttagtggt tatgtcagtt ggaatccgtc ctaacacagg ttttctaaaa gacacaggtt
     4381 tagaatttgc ccctaataat acaattattg cagatgaata tatgcttaca aatgacaaag
     4441 atatatacgt agttggagac tgtgcttttg taaaaaattc tttaacaaat aaacctacat
     4501 ggtcacctat ggggtcatct gcaaatcatg agggtagaat ttgtgcacaa aatatatctg
     4561 gaaaatctaa aacttacaat ggtgttcttg gaacaacagt agtaaaatta cctgagctca
     4621 atgcaggaaa aacaggtcta agtaaagaaa tagctgaaaa agaaggttat aatgtttgct
     4681 ctgtaaccat agctactgat gataaagctc attattatcc aggtgcatct aattttatta
     4741 taagaatggt tgctgaaaaa aatactagaa aattattagg tgttcaagtt atgggacctg
     4801 gagcggttga taaaatagta gatatcgctg ctactgcaat aactttaggc gctgaccttt
     4861 attcattaga aagtatggac cttgcatatg caccaccttt ttcaacagct attcaccctt
     4921 ttgtagtggc agtaaatgtt ttacaaaata aacttaatgg agaacttgat agtgttttac
     4981 ttgacgaaat tgaggatttt gacagttgga ctaaacttga tgtttcaaaa gctccttcta
     5041 ttcctagttt acgttatatt cctgttggtg aaattaatgg agaaattgaa ggtcttgcta
     5101 aggatgaaaa aattcttctt atatgtgcta aaggtaaaaa tagctatatg gcgcaaaatc
     5161 gcttaagacg ctttggctat acaaatacga aagttcttga aggtggaata ctattttatc
     5221 caaatcttaa ggtaatggag gagtaaatat gagtaatgtt acagcagaac aaaaaaagga
     5281 agtaaaaggt agaggttttc taccaagcag agatggagaa cattttgcag cacgtataat
     5341 aactgtaaat ggtgtaataa atgcatcaca agcaaaaaaa attgcagaag ctgctgaaaa
     5401 attcggaaac ggtcagttag catttactac tagactaaca gtagaattac ctggtgtaaa
     5461 atttgaagat attgaagctc taagtgaatt tattgctagc gaaaatctaa taacaggtgg
     5521 aactggatca agggttcgtc ctgttgtggc ttgtaaaggt actgtttgtg ttcatggtct
     5581 agctgataca caggcacttg ctgctgaaat acatgaagaa ttttacaaaa attggtatga
     5641 tgtaaaacta cctcataagt tcaaaattgg tgttggtgga tgccctaata actgtattaa
     5701 acctgaccta aatgatcttg gaattgttgg tcaacgtgta cctgactatg attcagagct
     5761 ttgtgttggt tgtaaaaaat gtgctgttgt agaggtctgt cctgttaaag cagctaaatt
     5821 aacagataaa ggtaaacttg aaatagatag taatctatgt aataactgtg gtaagtgtat
     5881 agaaagttgt aattttgata gtattgaaga aaaagaatct ggttataaag tctatatagg
     5941 agggaaatgg ggaaaatcag ttcgtcctgg aacacagatt gatagattat tttcaaaaga
     6001 ggaacttatg actttaattg aaaaagcaat tcttttattt agagaacagg gtaacacagg
     6061 tgaacgtttt ggaataacta tcgatagaat cggtgctgat aaatttatag aaatgctttt
     6121 atcagatgaa gttttggaaa gaaaagaaga gatactagag gctcttttac accttacagg
     6181 tggagctgta tgctaacaat aaatatttta attgtacaga taacctatgg aaaaaatttt
     6241 agttaagaat aattaaaatt ctatatatga tatatgataa atgaggctat ctcaaaacac
     6301 agatacgcct ctttttcatt gttgaaatga taaagtatat ttgagttcat ataaaaaaga
     6361 gtgtctcgtg aattataaaa ttcattttga ggcacccaca ttattttata ataattgctt
     6421 attaatttta ttttaactat ataaaatgta caaaaagtaa gaatatccaa tattctaaaa
     6481 ttaattttga attgtattga ttgaaaagtt tactattcca ctatttccat cttgatttac
     6541 tactagatac catgttccag tattaggaac tctcattcta catggatttt tctttgcaaa
     6601 tccaccatag taatcataat ttctttgatt tttaaatttc acataattta tatggtctaa
     6661 caatattaca ttggcttgtt tctctaaatt tacctctaca atatcccctt tttttaagtt
     6721 catcttacta taactatatt ccattgaatt actcctccag tatcgaataa tctttgttat
     6781 ttactcatct caaatatgta aaaattcatt taaaatatta caaatactat ataattagac
     6841 tattatccag ttttaacctt atttactaca atccaaaggt tattaaaaat attattggca
     6901 acaacttctc catcccatac aactctttct tctttatttt ctaataaagc atagtaccca
     6961 gagtcagaac tttcatcttt atagactgta acataaaact cttcatcact atattcaggt
     7021 atatttttaa agtcttcaga tattttacat gttaatatat aaccttctaa ttcttcatct
     7081 ataaacaaag caattcctcc aatctctata tttttactaa ttataccata ttatgtcata
     7141 aactttttat tttattacaa atttctattt tttctttact ttataaataa attatataaa
     7201 ttttcacttt ttaactttat attatactaa ttaattcttt tgttttccta tatatttctt
     7261 tataatttat aaatttagcc attaatttat acgattttat ataggtaagc atatactctc
     7321 acttaatatt aaataattta cattttttct atactataat catattcttt tgtcagttag
     7381 taattttcaa agtagattat ttcaattaaa atataataaa attgatttaa aaattataat
     7441 aaactaatac ttattaaaaa t
//
LOCUS       DAEORZ010000172         7451 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEORZ010000172 DAEORZ010000000
VERSION     DAEORZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.176"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            202..2061
                     /locus_tag="KRH39_003354"
     CDS             202..2061
                     /locus_tag="KRH39_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8513231.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2353..2952)
                     /locus_tag="KRH39_003355"
     CDS             complement(2353..2952)
                     /locus_tag="KRH39_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG8513232.1"
                     /translation="MSIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3055..3852)
                     /locus_tag="KRH39_003356"
     CDS             complement(3055..3852)
                     /locus_tag="KRH39_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-binding protein"
                     /protein_id="HBG8513233.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDGKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3965..4070)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4162..5148)
                     /locus_tag="KRH39_003357"
     CDS             complement(4162..5148)
                     /locus_tag="KRH39_003357"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG8513234.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVANISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIKHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5632..7362
                     /locus_tag="KRH39_003358"
     CDS             5632..7362
                     /locus_tag="KRH39_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBG8513235.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
BASE COUNT         2695 a          945 c         1123 g         2688 t
ORIGIN      
        1 taagagtaat aatctataag aacttatata ttagaaaaaa ttttgtatat atagaggact
       61 ctaaagatga actatgtaaa acaaggttta tatattctaa ttgtatagaa aatttattgt
      121 aaacgcatgt atattattag aaaaaatagt ttgaaggtat tcaataaaca taaaaaacaa
      181 tataggggat aaaggtgttt tatgaagaga tttttgagga gaattatctt agttttattt
      241 attttgttga tttttataag ttttatttct attaaattaa ttcataatgt tggagattat
      301 ggaaaattga ttaattatgt tgggattgtt cgtggagcta gccaacgtct aactaaacta
      361 gagatgaatc atcaaccaaa tgatgaatta atagaatata ttgatgaaat cctacaggag
      421 ttgattacag ggcatggaga ttatggttta gtaatcactg attgtaatga atacaatgag
      481 gacttacttt tattagaaaa aaagtgggaa gatttaaata ttgaaattaa aaaagtaaga
      541 atgaaagaac agaataacca gttattaagt ataagtgaag agtttttttc tcttgcaaat
      601 gatacagtgt ttaaaataga aaacttttct aaagaaaaaa gtaattactt aatgacttta
      661 ataataatta tatctattat aggtatatta gcatgtatca tattgatttt acaatatagt
      721 aaaaaaatgg ttagactgga aaaattaaat gtagatttaa aaaatattgc atataaagat
      781 gaactaacag gagtaaatac tatagaaaaa tttaaattgg atgcaaatca aaatatttgt
      841 atacataagg ataagaaatt tgctgtattc tatattgatt ttgaaaactt taaatacatt
      901 aatgatatat ttggatatga ttatggtgac atgatactga aaagatatgc taatcttatg
      961 atgaatgaca tagggaaata tgaaatattt gcaagagaaa ttgcagatag atttgttgcg
     1021 ttaagatgct atgtagataa ggaagattta gtggtaagac aacgagatgt agacaatgaa
     1081 ctaattaata taacaaatga aattaaaaat aaacatagta ttactgttgt tagtggaatt
     1141 tgttgtattg aagatgtaaa tgaaaaatta agtatagatg gtttaataaa tagagcaaat
     1201 tttgcacaaa aaactgttaa gaataaacca ggtactaatt atgcttttta taatgacagt
     1261 attcgtaaaa aaatgattga agaaaatact atcaagagta gaatacatga agctattgaa
     1321 aaaagagaat ttatagttta tttacagcct aaagttaatt tacatagtca aaaaataaat
     1381 tgtgcagaag ctttagttcg ttggcttact ccggataaag gtattatatc gccagcaata
     1441 tttattccag tacttgagaa gaattttttt attgccttag tagataagta tgtttttgag
     1501 gaggtttgta aatggattag aaagaggtta gatgaaaata aaccttttgt tcagatatca
     1561 gttaatgttt caagaataca attttataac actaaatttg tagaaactta ttccaatata
     1621 caaaataaat acaggatacc taaaaatact atagaaattg aatttacaga gagtgttgct
     1681 tttgagaatc aaaaacatct tttggaaatt atacatgatt tgcatgaaaa tggatttact
     1741 tgttctttag atgattttgg aaaaggttat tcgtctttaa gtgttttaaa agatttacca
     1801 tttgatgttt taaagcttga ttctatgttt ttcaaggcta gtttagataa agataaagag
     1861 aaaatagtaa taaaaaatat tgtacatatg ttaaaagaac ttaatatcac aactgttgct
     1921 gagggcattg aatgcaagga acaagttgaa tttcttaggg atattgggtg tgatttagtt
     1981 cagggatttg tattttataa accaatgcca atattagaat ttgaaaaaat attggataaa
     2041 gagtttgttt ataatagcta aaatgtactt tataaaatat gaactaatta agtaaaatta
     2101 ttttgtactg aataaatttt tactttattt acagaataat gattatttac agaaaagcgc
     2161 gaaaagtgta ttcctttaag tgtgggatgg atagcacaaa taggggagaa aaaatattgg
     2221 ccatataaga atgcataata atataaatat ttgaaaggtc agcattatat aaaaattaaa
     2281 aagaaaataa ataatttttc tataaaaaag agactatgag aaaagttact catagtctcc
     2341 taaataatgt atttattttc ttcctgcaac ccattttaat ccccaatgaa attcagtatc
     2401 catttctcta tgacctttga agtatttaac tactttttca acactgaatg tctcatcatt
     2461 tacattttct atcaatgcta ttgaacacat attatagcca tttttatgtt catcaagttt
     2521 gacaactaag tctgaatctt gtttttgttt gattgttaca attccatcag cttcagacca
     2581 gttagctaca ccttcgtata taaaagcata aataagtatt ctttttatat cttttatttt
     2641 attaccattt attcttaaat tttctccttg tgtattactt cctgttctgt catctccatc
     2701 taattgagca aaaggtggat tgtctaaact accaaaggca tttcctaatg cttgaacagc
     2761 accttttaca ccatttttca tctcaaatag gcaacccaaa tctaaatcta tattactgct
     2821 tcttaaagag ccaaaaaaac cctttttttg tactttttga ttccaattta aattaactaa
     2881 gatttcacct aaatcgctat tttccttttt tgtaaggttt attttctgtc ccttttttaa
     2941 ttctatagac atagtaatct tcctccacaa acatagattt atttaaatgg tagcacttaa
     3001 taagtcaata ttcaacaaga ttagaataat aatattaaaa ttatactaaa tatactaaaa
     3061 agtaggaatt acaatacctt tatatttttc ttcaattaag cttcttgctt tatctgatgt
     3121 aagaacatct tgtagaactt taatcttttc actgtcttta tcttcagttc taaatgctaa
     3181 aagatttgca tactcgtcat ttataggagc tgtataaatt ccgtttttct tggcatcaag
     3241 accagcaggt acagcataag tagtgtctat aaatgcagca gtcacatctg gaagagaaga
     3301 tggaatttga gcaatatcta actctttaaa ttgtattccc tttggatttt ctattatgtc
     3361 cttaggacta actaatccat cccccttagg taacgttaaa agtccaatac tttcaaggta
     3421 tctaaggttc ttagaacaag aagatgggtt attgcttatt gcaattgtat ctcctttttt
     3481 aaactcatca acagatttta tcttataaga gtaaagtatt gctgggcata catatagttt
     3541 tttaccagcc attattttgt aacccttggc cttaacagct tcttttagat aaggttcatg
     3601 ttgaaataga tttgcatcaa tctcaccatt atttaaagct tcgtttggaa gtatgtaatc
     3661 atcaaagttt ttaacttcta aagtatatcc tttttcttta ataagtggtt ttaattcttc
     3721 taataactct cctcctggaa ctaaagtagc accaacaacg atttttttac catctttagc
     3781 tttagaacat ccaaccacag ctaaagttaa tactagacat aataacaaac ataataattt
     3841 tttgaatttc atataaattc cctcctgaat ataattgatt agttaaaaat aaaataccat
     3901 tctttatcac actgcaaaat aaaaataagc ctgagtttaa actcaggctt tagaagctaa
     3961 tttagactca tctttcagta taatactgca ggatttagca ccaatgtata taaaaatata
     4021 ctggttgctg ggtttcatag ggccagtccc ttcaccactc tggataagaa gtatcaattt
     4081 ttaattatgt tataaatatt atcatgattt tttacgtctg taaacataat tttataaata
     4141 ttttaaaatc tttaatgctt tttacttaac acctaaaatt ccagaaacta gaatatcagt
     4201 atcagcacct aagaaataat catttatttc aatagaatcc agagtatttc tcaaagcatt
     4261 ttcttcatgc tttataccaa taagtttatt ttctacaaat gcaacatcac attttccaaa
     4321 aaaatctcca aaaaatttta tactagttat tatgccttta tcaacattta aattaaactc
     4381 aacatttcca ccagaatatt ttaattcatt tgaaagagta tattttggtg aattaccaaa
     4441 attccaatcc caagtttcgt attttgattt agtaagcttt tctatttgtt ttatatcatg
     4501 ttcactcaaa caataattag tattatcagt atttgtttta gaaagataat ccattatagc
     4561 atctttaaat tctaatatgt ccattttaga gtgtaaatgt tcacttatat tggctacacg
     4621 agattttaca gattttattc cttttccttc aaattttata ggtttaactt ttaatgcttt
     4681 tgagaggtca tttatttcag atgaaaataa aagtgtacca tgatgcatta ctttgttttt
     4741 gtagttgtat tgagcatttc cagaaaattt cttgccatct ataagaaggt catttcttcc
     4801 agaaaattca gcattgacat ctagtgtttt aagtaaatct attatgggtt gagtgaatct
     4861 tctgaaatca ctaaaattat tattgttaca agctataaat gtaaaattta tatttcctaa
     4921 atcatgaaat acagctccac caccagactg tctcctaacg acaggtatag aatattcttt
     4981 tatatattct aaatttattt cagaaagtgt gttttggttt tttccaacaa ttatagaagg
     5041 ttcattcctc cataaaataa ataagtcttc atcaaaattt tttagaagat attcttccat
     5101 tgctaaatta aaataagggt ttgttttttc attataaatt aataacatta aagtagttcc
     5161 tccatagaat tgataaaatt tttattatga atacaattaa ttttataatt cgacaataat
     5221 ataaaaactc ctttaaatta gaaattttaa aaactacctt gcaaagttta aataaataat
     5281 atatactcaa aatatgttta ataaaagtga atagaagggt agaggcgcaa taatctatca
     5341 gtactaataa ggagttaagc acagagattt attaggaaag gagatattgc cgaagtttta
     5401 taatcgatgc tttaagatta tattgctggg ggtacataga aaatatgtac aactgtcaca
     5461 ggtttatttg tggtgagcta tcatttaagg aattatatta tatttatttt tactattgtt
     5521 gatatattgt aatgtttaag ccttgagtgt ttaccactca aggctttttt agttttattg
     5581 taacataata ccctgtacag ttcacaaaat taaaaggaga gggagattta tatgaggaac
     5641 aaaaaaatta atattatttt tctgacgaca atcatgttca taatgtcaac agtaatggtt
     5701 tttgcagaag aagatataga cactattgca ctagccaatg cagaaaaatt tgggatttta
     5761 actttaatac caccattagt tgctataata cttgcattta taactaaaaa cgtaataatt
     5821 tctttactta taggtattct ttctggtagt tttataatta aagctagtgg tattaatgtt
     5881 tttgcgacat ttatacaggc atttttagat ttagtagaca gagctttagt atctttagca
     5941 gacccttgga atgcagggat aatactacag gttttggcaa ttggaggagt aataaatctt
     6001 gttgctaaaa tgggaggagc taaagctata gctgaagcac ttgctaaaag agcaaagtca
     6061 gcaaaaggga cacagcttat aacatggttt ttggggcttt tagtattctt tgatgattat
     6121 gccaattcac taatagtagg tcctatgatg agacctgttg cagataaaat gaaaatatcg
     6181 agagaaaagc tagcatttat aatagatgcc acagcagcac cagttgctgg tttagctata
     6241 atatctacat ggataggtct tgaggttggt ttaatacatg atgcttttga aagtataagt
     6301 atagatgtag atgcatttgg tatattttta aatacaatac catttagatt ttacaatata
     6361 ttaatattag ctttcattgt aatatcagca ttattattaa aagaatttgg tccaatgaga
     6421 aaagctgaga taaaatctag aagtagaaaa attagtatag accttgatga aggcgtagaa
     6481 gagttggatg atttagctcc aaaaaatgga gtaaaattga gtgtatggaa tgctataatc
     6541 ccaataggaa cattgataat agttgcatta gcatcatttt attatagtgg atatacatct
     6601 ataatgggag gagacgataa ggctctgata caactattta caaattctcc atattctttt
     6661 gaagcaataa aagaagcatt tagtgcatct gatgcatcta gagcattatt ccagtctgca
     6721 cttgttgcaa gtttagttgc tattataatg gctgtagtaa aaaagatttt tacaatatca
     6781 gaagcaattg atgtttggat agatggaatg aaaagtttag taatcactgg tgtaatatta
     6841 atacttgcat ggtcattaag ttcagtaata aaagagttag gtacagctaa atttttaata
     6901 cacctattat caggttcact accaccattc ttattaccaa gtttaatatt tggtttagga
     6961 gcaataatat cttttgctac tggaacagct tatggaacaa tgggtatact tatgccactt
     7021 gcaattccat tagcatactc attaaatcct gatatgtctt atgtaatagt aagtactagt
     7081 gcagttttaa caggtgcaat atttggagac cattgttctc caatttcaga tacaacaata
     7141 ctttcttcaa tgggagctgg ctgtaatcat atagaccatg ttaacactca aatgccatat
     7201 gctatattta cagcagtaat cactattgta tttgggtata taccagctgg acttggattg
     7261 ccaatatata tagtattacc agtggcaata gctgctatat ttgtaggtat tcaaataatt
     7321 ggtaagaaag ttgatgaagc ggaaatagaa cttgtagaat aaagatataa ataagtttca
     7381 ccttttatta tcactatttt gttagttttt agataatttt aaaaagttat tgaaaataat
     7441 ggcgaaatat a
//
LOCUS       DAEORZ010000173         7278 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEORZ010000173 DAEORZ010000000
VERSION     DAEORZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.177"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            155..1855
                     /locus_tag="KRH39_003359"
     CDS             155..1855
                     /locus_tag="KRH39_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513236.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            2218..3405
                     /locus_tag="KRH39_003360"
     CDS             2218..3405
                     /locus_tag="KRH39_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513237.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            3465..4628
                     /locus_tag="KRH39_003361"
     CDS             3465..4628
                     /locus_tag="KRH39_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8513238.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            4779..5582
                     /gene="zupT"
                     /locus_tag="KRH39_003362"
     CDS             4779..5582
                     /gene="zupT"
                     /locus_tag="KRH39_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBG8513239.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAAIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            5677..6237
                     /locus_tag="KRH39_003363"
     CDS             5677..6237
                     /locus_tag="KRH39_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBG8513240.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            6488..7201
                     /gene="rgbR"
                     /locus_tag="KRH39_003364"
     CDS             6488..7201
                     /gene="rgbR"
                     /locus_tag="KRH39_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBG8513241.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
BASE COUNT         2763 a          757 c         1338 g         2420 t
ORIGIN      
        1 caactatatg atataaatct tcaattttat gaaaaagatt gttattactt tgtaagttta
       61 taaaagattt aaagttattg ttatgttata ctaatttatg ggtatttttt agaagtacta
      121 atagaagtac taataaagga gggatttaat acttatgata aaacaatttg tcaaatatta
      181 taagccatat aagaaaattt ttacactaga ccttatagca gcttttctat tttccttatg
      241 tgatttagtt tatcctatga taactagaaa tataatggat gacgttgttc caaataaaaa
      301 cttaagaatg ttagtagttt ttgcagtagc acttatactt atatttatag caaaagcagg
      361 gcttaactat tttatgcagt attggggaca tgttattggt gtagatatgc aagctgatat
      421 gagaaatgag gtatttacac acttacaaag acttccaaat acgtattttg ataataacaa
      481 atctggagta actatgtcta gaatagtaaa tgatttaatg gatataacag aacttgcaca
      541 tcatgggcct gaagatttat tcatttctat agtaatgttg gttggttcat tctttatact
      601 tatagatata aatattcctc ttacgcttat aatatttgct atattaccgt ttataatttg
      661 gtttgcaata gctaaaaaag ataagatgaa tattgctttt atgaagagta gagttactat
      721 aggtgatgta aatgctactt tagaaaatag tatagcaggt atgaaggtaa ctaagtcttt
      781 ttgtactgag aaagaagagt taaataagtt tgtaagaagc aataaattat ttagaagagc
      841 aagacaagat tcttataagg taatggcaga atattactct ggtatgaatt tatacatgga
      901 tatcttagag tgggtagttg taatagctgg tggttatttt acttatatag gaaagattac
      961 tttgggtgat tttgctgctt atatactata tgtaaaaatg tttatacaac ctatgaaaaa
     1021 attaataaac tttacagagc aatatcaaaa tggtatgaca ggttttaaaa gattcataga
     1081 gataatggaa caagaccatc aaaaagaagc taaaaatcct attgaacttg aaaatgtaaa
     1141 aggtgacatt gagatagaaa atatatcttt cacatatgaa gataagactc aagttttgga
     1201 taatttaagt ttatctataa aagctggtaa gactatagca ttagttggac cttctggagg
     1261 aggaaagact acattatgta atctattgcc aagattttat gaatttgata agggtgatat
     1321 aaaaattgat ggaaagagta taaaagatgt tagtttaaaa tcattgagaa aaaatatagg
     1381 tatagttcaa caagatgtat ttttatttac aggaactata agagataata ttctttgtgg
     1441 gaaccctaat gctacagatg aagaaatgat agctgctgct aaaaaagcta gaatacatga
     1501 ctttgtagag actttgccag atggatatga tacttatata ggagaaagag gcgtaaaatt
     1561 atcaggtggg caaaaacaaa gaatttcaat ttcaagaatc tttttaaaga atcctccaat
     1621 aataatattg gatgaggcta catctgcttt agacaatgta acagaaagag aaattcaaga
     1681 gtctttagag gaattaagta aagatagaac aaatttagtt gttgcacata gattgactac
     1741 tataaaaaat gctgatgaaa tcatagtatt gacggataaa ggaattgaag aaagaggtac
     1801 tcatgaagag ctagtaaaca aaaatggagt atatagtagg ttacacaata actagtatgc
     1861 aaattttaca ccatagctaa catacacatt aaacttacaa acataggata tattaagatt
     1921 cataaagaca attcatttta tatttataag atgttttatg aataaaatgt atactatgtt
     1981 ttattttttg caaattacat ttaattctct ttgtaagaat agagttaaat agccaatggt
     2041 cgggataacg tttttacaaa aagaataatt tagaaaacta actttaattt atggaattta
     2101 tcgaatttta tgaaaaaaat cgaattttga cttgaaatgt ttttcaaaag tagtaaaata
     2161 atggataact atacatacat tttataaaaa tatatttatt atgagggggg attttgtatg
     2221 gaggcaataa cagctttttg tctagtttta gtatcactga cgataggaga tatagtttca
     2281 gcaaaaacta aagcttttgt tccgtcggtt tttgtatcag caattatatt tatagtagga
     2341 ttttggactt ttttcccaga aaatattgtg gacttagccg ctttaggaac accattagct
     2401 caattaggta tgcttttgtt aataactcat atgggaacta tgatgagtat taaggaatta
     2461 gcaggtcaat ggaaaacaat agtaatagcc ttggcaggaa tagtaggaat atgtgtaggt
     2521 gctttggcat tagggacagt agtatttgga tgggatacag cagtaatagc tacacctcct
     2581 cttacaggag ggctagttgc atctataatg atggcagatg ctgctacagc aaaaggtctt
     2641 gcaagtttat cagtacttgc aattttgatg tatgttgcac aaggatttgc gggttatcct
     2701 attacagcat taatgcttaa aaaagaagga aaaagacttt tatcagactt tagaagtgga
     2761 aaagtaactg taaatacaga tgaagaaaaa gttaaggatt tacctgaaca aaaaagcaga
     2821 tttaagataa taccagactt accagaaaaa tatgatacga catatatgat aatacttaga
     2881 ttaggatttg tagcatggtt agcagtacaa tttacaaatc ttacaaatga agttgtatca
     2941 agatatgtta tatgtttaat atttggtgta atagcatcag aacttggaat agttgataga
     3001 aaaccactta ataaatctgg ttcatttgga tggttaatga catcattgat ggcttttata
     3061 tttgcagggt tagcaaaagc tacaccagca atgcttgttg aaatggcagt tcctcttgta
     3121 ggaatcataa tatttggtgt aataggtatg ggaatattct ctattatagt aggtaagctt
     3181 ttaggtgaaa gtaaagaaat ggctttttca gtttcgctta catcacttta tggttttcca
     3241 ccaaactata tattaactga agaggcagta aaggctttgg ctgaaacacc agaagaaaaa
     3301 gaatttttaa tggataagat gcttccaaag atgcttgttg gaggatttac tacagttaca
     3361 atagcttctg tagtaatagc gggtatcttt gtaaatctaa tatagtattt aagtgataaa
     3421 aagtatttta aataagaaga atgtgtacta cttggaggta tggaatggat attaaagaaa
     3481 taacaaagag ttataaagac tatgtaatta agcttagaag ggaatttcat gaaaatcctg
     3541 agaaaagtat ggaagaagtt agaacatcaa agagagttaa agaagaactt gataaaatag
     3601 ggataccata tgtatcagct ggaggtacag gtgtaattgc aactataaaa ggagctaatc
     3661 caggaaagac tgtagcactt aggggtgata tggatgctct acaagttgtt gaatgcactg
     3721 atgtagaata caaatctaaa aatgaaggac ttatgcatgc ttgtggtcat gatggtcaca
     3781 catcaatgct tctaggggca gcaaaagtgt taaatgatat taaggatagt atcaatggaa
     3841 ctgtaaaatt attttttcag ccaggagaag aagttggtaa aggtgcaaga gctatgattc
     3901 aagatggtgc tatggaaggt gtagatagtg tgtttggaat acatctttgg actgatgtag
     3961 aatctgggac catatcggta gaagaaggac caagaatggc ttctgctgat ttctttaaaa
     4021 ttactgtaaa aggtagaggt ggtcatggtt cacttcctca tcaaggtgtt gatgcagtgc
     4081 ttgcaagttc agcaatagtt atgaatctac aatctatggt tagtagagaa gtaagtcctc
     4141 tggaaccatt agttgtgagt gttggagttt taaattctgg aactagattt aacgtaattg
     4201 cgagtgaagc tgtcttagaa ggtacaataa ggctatttaa tccagagctt agaaagcaaa
     4261 ttcctggaat tttagaacgt atagcaaaat ctacagcaga agcatataga gcagatgctg
     4321 aattagagta tgggtactta acaccagcag ttataaatga caaagaatgt tcaaagatag
     4381 ctacagatgc agcaattaag ctatttggag aagactgtat aacactattt gaaaaagtaa
     4441 caggagcaga agatttagct gagtttatga atatagcacc tggtgcacta gcatttgtag
     4501 gagctagaaa tgagtcaaaa ggtgcttgtt atcctcatca tcatggatgc tttaatatag
     4561 atgaagatgc tctagaaatt ggaactgctt tatacgtgca atatgcagtg gatttcttaa
     4621 ataaataatt ttaatatact ataaagaaaa agcagagact aagtttatat ctctgctttt
     4681 ttattatgta tattttttat aaatattgac tatataaaat atatatgata gcataaagaa
     4741 aatgataatc aatatcaatt aaaaagaagg tgatttattt gaataatgta atatttgcat
     4801 ttttgataac attattggct ggtttatcta ctggtatagg aagttgtata gcattttttg
     4861 ctaaaaaaac aaatactaag tttctttcta taagcttagg attttctgct ggtgttatga
     4921 tttatgtttc tatgatagaa atttttccta aagcacaaga tgctttgaca aaatctatgg
     4981 gagagaaatt aggaagttgg tcaactgtaa tagctttttt tataggtatg gctatcattg
     5041 ctgctattga taagctaatt ccacaagaag aaaatccaca tgaaataaag aaaatggaga
     5101 atatagaaga aaagaatatc aaaaaaaata agtctctttt aagaacaggt atatttacag
     5161 caatggctat agcaattcac aattttcctg aaggattagc tacatttata tcagctcttg
     5221 atgatgttac aatagctatt ccgatagcaa ttgcaattgc aatacacaat ataccagaag
     5281 gtatatctgt atctgtacca gtttattatg ccacaggaga taaaaagaaa gctttttatt
     5341 attccttttt atctggtatg tctgagcctt taggagcgat tattggatat gtacttctta
     5401 gaaatttttt aaatgatata actcttggaa ttgtatttgc aattgtagga ggaattatgg
     5461 tattcatatc actagatgaa ttattgccat cagctagaga gtacggggaa catcatttat
     5521 ctatttatgg attgattgca ggaatgggtg ttatggctat tagtttatta ctatttaaat
     5581 agcttttaaa tatatgattg taacctaaaa ttagtatcaa aatgatataa ttatttaaat
     5641 agatttttaa gctatttaaa aatatcattt tggagaatga gtgctatgga aaagaaaata
     5701 aaattcaaat ttgactggta tagttcacct gataagatag agaaatggtt agaagatatt
     5761 gaagctaaag gatataattt atataagata aataaatttg gaggaatatt ttattttcat
     5821 aagggaagtc caagaaaaat aaaatacact gttgattact actatgatgt agagaataat
     5881 tattttgatg aacataaatc aaatggctgg aatcttgtat ttaggagtat tggtagtttt
     5941 atgggtacat ctgcatggta tatatggtcg aaagaataca aagacatacc tcctaaatta
     6001 tatagtgatt ctgataaaac aaataaagta aagtcaattt tatttatgaa tatgtttata
     6061 ttctttatgt tagtagtaat atttatatat ttattgaata ttgaaataag aaatttatat
     6121 agtggttatg gagcatctgt aactgctatt gtactacaga ttatattgtt gattttatat
     6181 gcagtacaat tatataggag attaatgtat tatttaaggc ttaaaaagtt taaataaata
     6241 aaagaaggtg tcctaaaatt atttttttaa tattttaaga catcttcttt tgttaataaa
     6301 gtactgtttt taattatagt aatctctgtc tttaaattga aatatcaaag aatagttata
     6361 tgggattgag tattgataaa aataatttga aaatgttatc ctaaagtatc agatatacaa
     6421 aattatcttt aaatatatta taaatatttt aattgtatta actagaaatt atgtaaggag
     6481 gcattttatg attaagatag cagtttgtga agatgaaaaa gaaacacaac ttttaataga
     6541 agattacctt gagaatatat taaaagatat aagtatagaa tatgagatac aaaagtatat
     6601 atctggagaa gagttactgg aaagtaattt aaaagatata gatatattac ttcttgatat
     6661 aaaaatggaa aaactaaatg gaatggatac tgcaagaaag attagagaag tagacaatga
     6721 aatggaaata atatttgtaa cttcattaat agattatgtg caagaaggtt atgaggtaag
     6781 agcttatagg tatttactaa agccaataga gttggaagaa ctaaaaaaac atgtgctaac
     6841 ttgtatcaaa gatattgaaa taaataagga aagtcatatt acaataaaaa ataagtctaa
     6901 tacatataag atttatttaa atgaaataaa atatatagaa gttcaaaaaa aagatatgct
     6961 aatacacaca ataaacaaga attttgatat aaaatatagt ttaggcaaaa tagaaaaaga
     7021 attaaatcca tataaattta taagatgcca taaaagtttt atagtaaatt taagatatgt
     7081 tgaaaatata aagcctaata ctgcaatact ggaaagtgga gaagaggtac ctattagtag
     7141 atatagatat aaagaagtta aggagaaatt cttaaaattt cttggtgata caatatgcta
     7201 aactttatga ctgagctatt agtagtttta tctatattta tacagttttc tataatgaac
     7261 ggaattttaa tttataaa
//
LOCUS       DAEORZ010000174         7141 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEORZ010000174 DAEORZ010000000
VERSION     DAEORZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.178"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            47..388
                     /locus_tag="KRH39_003365"
     CDS             47..388
                     /locus_tag="KRH39_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBG8513242.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESANYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            539..952
                     /locus_tag="KRH39_003366"
     CDS             539..952
                     /locus_tag="KRH39_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBG8513243.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1154..1795
                     /locus_tag="KRH39_003367"
     CDS             1154..1795
                     /locus_tag="KRH39_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG8513244.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYLIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1819..3072
                     /locus_tag="KRH39_003368"
     CDS             1819..3072
                     /locus_tag="KRH39_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG8513245.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3118..3768
                     /locus_tag="KRH39_003369"
     CDS             3118..3768
                     /locus_tag="KRH39_003369"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBG8513246.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3819..4415
                     /locus_tag="KRH39_003370"
     CDS             3819..4415
                     /locus_tag="KRH39_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG8513247.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4428..5267
                     /locus_tag="KRH39_003371"
     CDS             4428..5267
                     /locus_tag="KRH39_003371"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBG8513248.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5512..6666
                     /locus_tag="KRH39_003372"
     CDS             5512..6666
                     /locus_tag="KRH39_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBG8513249.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            6972..>7141
                     /locus_tag="KRH39_003373"
     CDS             6972..>7141
                     /locus_tag="KRH39_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513250.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNV"
BASE COUNT         2967 a          667 c         1253 g         2254 t
ORIGIN      
        1 aaaaaacact aaaattaaat taataaaagt acttggaggg atggaaatga aagaggaaaa
       61 ttttattaat gttgaatacg aggataaacg tttttcaatt aatagtaaag aagttttgta
      121 catagaaaag caagagtatg aagtaaagat ttatactgaa agtgctaatt atgaagttgg
      181 agaaaatgct ccttgtataa gaaaactatt gaaagaagaa aattttttca aatgcagtaa
      241 tagttatatt gtaaatctag acaagtgtga attttatctc agcaataatt gtatttttgt
      301 gaataataat tttataagaa ttttaggttc agaaagaaaa aagaaattaa aaaagaaatt
      361 agaaaaatta tatgattatg aagattaata ttgggaggct gtattttata cagcctcttt
      421 atttattgat tgtattatta ataaatttta aattatgata aaatataaaa caagtaactt
      481 atttcagaat aaaatacttt ttaaaagaaa gggacgagat atgggagatt ataataaaat
      541 gcttgaatta aagaataact taggtatcag tgaaagaaag ttaaaatatc cttgtatcta
      601 taaacatttc aaagggaaat attatgctac aatgggtctt agcaaggcta ttgatgatat
      661 agaaaacata tgtgaaatat atggtaaaga aaatttaatc caaaacagaa ataaatataa
      721 attagttatt agacatactg aaagagaaga agatatctat gtttataggg atttagatgg
      781 aaacttttat cataaaaaag aagaagatac taatgattta gttttataca agaccttata
      841 tgatgataca ggaatatttg caagaccttt ggatatgttt ttagaaaaag tagatacaga
      901 taagtatgtc aattcaattc aagaatacag atttgaagaa gtatataaat aatataagag
      961 aaatatgttt ataaatttag aaaaattctt taacagtaca ataatttgca gaaatacaaa
     1021 tgagatttat tgtaaataat atgttagatt agattataaa atagaaacaa agtaaagtat
     1081 tatatgatat aattaagttt tgagaagtga taatttatat ttaataaaaa ataggcatat
     1141 attaggagga catatgggaa atacttttta tatagtaaga catggacaaa cggattggaa
     1201 tatattggga aaaacacaag gtcatggtaa ttctgacctt acgcctcaag gtatagaaca
     1261 ggcaaaagaa ctttctgaag atattggcaa gtatttaatt gattatattt ttagtagcga
     1321 ccttgggaga gcaatgcaaa cagctcaaat tttaggagat aagctcaata ttgaagttca
     1381 aaaaactgag gcacttagag aaatgggatt cggagtttgg gaaggccttt taataaaaga
     1441 aattcaaaaa gattattcag atatatatgc aacatggaga aatgaaccac acttagtaaa
     1501 tataccagaa ggcgaaactc ttaagattat aaaggaaaga gtagatgctt ttataaagga
     1561 actaaatgaa aaatatgata ataaaaatat aattcttgtt acacattcaa ttacattgag
     1621 ggttatgttg ttgtcatttt tagaatcagg tatggaaaat atatatagaa ttaagcaaga
     1681 taatactgct ttaaatatag tagaatttaa agattatggg cctgttatag taaaaatgaa
     1741 tgatacaagt catataaaaa atcatgtaaa gataaataat tctgcactgg agtaggaatc
     1801 aattataagg agaaatttat gagtaaagtt atagtagttg gtggaggagc ttctggaatg
     1861 atggcagctt tgtcagcttc aaaaaacaat aatgaagtaa tattagttga gagaaatgga
     1921 gaattaggaa gaaaacttag ggcaactggt ggcggtagat gcaattttac caataataga
     1981 gagatagaag atttttttga taaagttgtc agtaataaaa aatttctcta tagtagtttt
     2041 tatacattta caaataaaga ccttatttcg tattttgaaa gtagaaattt ggaatataag
     2101 attgaagaag aaaatgacca caaagtatac actaaaaatg ataaatctat agaagtaata
     2161 gaagtattaa ataaagattt actcaatcac aatgtaaaga taatgtataa taagaaagtt
     2221 atagatatta taactgaaga aattgctcta aaagatgatt ctaataagga caagtcaaag
     2281 tatctaataa aagggattat tttagataat ggagataaaa tactaggaga caaggttatt
     2341 atatcaactg gaggagtgag ttattcaaag acgggttcag atggttcaat gtataagatt
     2401 cttaaaaagc atggacatac attaaacaaa ctatatcctg ctttagttcc acttacaata
     2461 gaggaaaaat ggattaaaga tttacaagga atatccatga agaatgtgga aatatcttgt
     2521 aagattaaga aaaggaaaat atcaaaatct ggagacatgc tatttgccca ttttgggata
     2581 acaggacctt gtgttcttat aatgtcttct tatattaaca agattattga aaaggaaaag
     2641 gtagaattaa atatagattt cttaccaaat ttaagtactg atgaaatctc tagtataatc
     2701 agagcatttc caaataaaaa tgttctaaat aatcttaagc aaatactacc tcaaaatttc
     2761 ttaaaagaga ttttttctct attatcttta gttgataaaa aggcaagtga tttgtctaag
     2821 gcagatgaaa ttagaattat tgagtatata aaaaatatga agctaacttg taatgggact
     2881 acaggtataa acactggtat ggttacttct ggtggtattt cagttaaaga aattaattct
     2941 tctactatgg aatctaaatt agttaagaac ttatttttta caggtgaagt aattgatata
     3001 gatgcagaaa ctggtggata taatttacaa atagcatttt caactggata tcttgcgggt
     3061 ataagtgtat aaatataatt ttttaataat taaatatgaa taaatagagg tgtatatatg
     3121 ggtaatttag ttatagctgt agatggtcca gcaggagctg gaaaaagtac aatagccaag
     3181 atagtagcaa aaaagctaaa tataaattat atagatacag gtgctatgta cagagcagtt
     3241 acttataagt gtttaaaaag tggtattgat gtcaataatg aaaaagaagt tattcaaata
     3301 gctgaaaatt cggatataga ttttaaagat aacaatatat atttggacaa agaagttata
     3361 aatgaagaaa taagaacaat agaagtaagc aataatgttt ctaatgtagc aaaaataaaa
     3421 gaagtaagac agttgatggt tgaagttcaa cgaaaaatag gaatgaaaaa ttcagtaata
     3481 ctagatggta gagatatagg ttcatatgtt ttcccagatg cagattacaa attcttttta
     3541 gtagctactc cagaagaaag aggaaataga cgctataaag aactttgtaa taaaggatat
     3601 aatactactt tagaagaagt aattgaggat ataataagac gagatgaaat tgattcaaat
     3661 agagaatttg caccattggt aaaagctaat gatgcactag aaatagatac tacaggaaaa
     3721 actatagaag aagttgtaga agaagttgta tctaaaataa atctttagtt ttttatgcat
     3781 cataatgaaa ataaatgtag tgttgaaggg agataaacgt gaatttttat agatttgtaa
     3841 taaatatttt taagggtttt tctaaaatat tttttaaata tgaagttata ggtgcagaga
     3901 atattccaga tagaggaaat atagtaatag catcaaatca taaatcgaat cttgacccta
     3961 tattcttggc tgctgccata gaaaatagag aaatagctgc aattgcaaag aaagaattgt
     4021 ttaaagtaaa accattggga tttatattaa aaaaattgca tgttatgcct ataaatagag
     4081 aaaaaccaga tgtatctaca ataaaaacta ttttaaggtc agttagagac ggttatgtat
     4141 taggaatatt tccagagggt actagaataa aaggagattc ttttggtaag gctaaggcag
     4201 gtctttctgt atttacaata aaaagtaaat ctaaagttgt tccagtatca ataatatcta
     4261 aatataaact ttttagtaaa gtaattgtat atattggaga accaatatca tttgaagaac
     4321 attttaagga aaaattgagt aatgatgacc atgaaaggat atcacaagag attttagaag
     4381 tgataaaaca aaattacttt aaatattcaa aataaggggg taaagttatg aacataaaaa
     4441 tagcaaaaaa tgctggtttt tgttttggag taaaaagagc aatgaaaatg gcatgggatg
     4501 aagttgaaaa aaatgattct ggcatatatg ctttaggtcc tcttattcat aacaagcaag
     4561 cagtagcaaa atatgaagaa aaaggtctta agactgtgaa tgaaattgat acaataccaa
     4621 atcatgaaaa tatgataata aggtctcatg gagttcctga aaatatatat aaggaagcca
     4681 aagataaaaa attaaaaatt gtggatacta catgtccatt tgtaaagaaa atacatacag
     4741 tagtaagtga atatcataat aaaggctatg aaataatagt tattggagat atgaagcatc
     4801 cagaagttat aggtataaat ggatggtgtg aaaattcagc tataataata aagaccttag
     4861 aacaaatgga aaatatggaa tttgataact ccaaaaaata ttgtcttgtt gcacagacta
     4921 caataaatcc agagttgtat ataagtatag ttaataaact atccgataaa ttagaagaaa
     4981 tagtatttaa tgatacaatt tgttcagcta caaaaacaag acaagaatca gctaaagagt
     5041 tagctaaaga agttgattgt atgatagtta taggaggtaa acacagttca aatactcaaa
     5101 agttagtaaa ggtttgtgaa gacttagtac caacttttgc aatagagact aaagatgaat
     5161 tggatgtaaa tacgcttaaa aaatataaaa atttgggtat tacagcaggg gcatctacac
     5221 ctaattggat aatagaagaa gttgtaacat tccttgaaaa tttataattt gataggtaat
     5281 aaaaataggt ggacattgtt ataatttatc ataatatcca cctttgattt catattacat
     5341 gtggtaaata gataacctaa tatcaaaaag tgtgtatctt aagatgaaaa actaagagta
     5401 taagatgcgc actttttaat attagaaatt caaaagaaat gttgatattg attatcaata
     5461 gaaaatagaa tataatataa ttgttgcaaa attagtctag gaggtaaatt aatggaaatg
     5521 ttgatatccg cttctgaaga ggcgttttta catgtaggtt ctatgattgg attttttatt
     5581 ttgttatttg gatatattaa ttataaaact agtggaaatt ttactaatat catatcaaaa
     5641 aacagaaaat ttcaaccctt aataggtgct ttaattggag ctataccagg ttgtggcgga
     5701 agtttggcaa taatgccatt gtatataaat ggaaaattaa gctttggagc aataattgca
     5761 agtttaatag ctagtatggg agatgcagca tttgtactta tttcatcaaa tattaaaatg
     5821 tatttttttg tcactatagt tagtacaatt acaggaataa taactggaca attagtggat
     5881 tactttaaac tagaagaaaa attagggtta aaaaaccgta gaaaatcaga taaatatgat
     5941 ataagtaaaa ataaccctaa agaaaataaa gaacaacata atcatgatga aataatttta
     6001 gacactctag ctaaatctca tggaaataca aatagacttg catttataat aacacatgga
     6061 aagggatata aaatttatat aggaattata gtaatcggtt ttatatttat gtcacttgct
     6121 cactctggac ttaatcttcc tataattgag aagttacatt cattggaaga agtaatagca
     6181 gtgattggaa tattattttc aataatttat atgtggtgtt ttaaaaaggt atttaaaaat
     6241 tctaatcaaa aagaggaaga aaataaaaag atttctttgc gagaaatgct tatccattct
     6301 gttggagaaa tatcatttgt aattacatgg atttttattg catatcttat ttacgatttg
     6361 ataatattaa ttttaggtgg agatgaatac ttagttaaat tagtattatc aacaggtgta
     6421 ataagtgtat ttataggtgc aggtcttgga cttattccag gttgtgggat acaaattgtg
     6481 cttatgtcat tttatttgaa gggaactata ccacttggtg cagtgatagc caactctatt
     6541 tctcaagatg gagatgcact tttcccactt ttagcaatgg ataaaaaatc ttctttatgg
     6601 gctatgatta ttactactat accagcaata cttgtaggta tgatagttta tgcattttta
     6661 ggttaaatat aattatgaat aaagaagtat taaaaaaata ataaagcaca taaatttatt
     6721 tataagaata gtgaagatta aatataaaaa taaaaaattc aatatgaatt aaattattgc
     6781 ttaaatatta atgagaacat gggagataat attattcctc atgttctctc tatttaatta
     6841 aaggaattat ggaacaaatt tcaagtgata ctttctatca aaactcatat ataatatata
     6901 ttaataaatt aaactataat acgtaataca gtcagtatat tgatataaaa aacttaggag
     6961 gagttgtaaa aatgggtaat ttaaaaagac agtgtgatgt ttcaagtgga agagaaaaag
     7021 ctgacattgt actaaaaaat gggacaatca taaatgtatt tacagaagag ttaataacag
     7081 gagatgtagc aatagtagga gacactatcg ttggaattgg cgattacaag ggcaatgtag
     7141 a
//
LOCUS       DAEORZ010000175         7138 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEORZ010000175 DAEORZ010000000
VERSION     DAEORZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.179"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            420..929
                     /locus_tag="KRH39_003374"
     CDS             420..929
                     /locus_tag="KRH39_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBG8513251.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            957..1151
                     /gene="rpmI"
                     /locus_tag="KRH39_003375"
     CDS             957..1151
                     /gene="rpmI"
                     /locus_tag="KRH39_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBG8513252.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1206..1562
                     /gene="rplT"
                     /locus_tag="KRH39_003376"
     CDS             1206..1562
                     /gene="rplT"
                     /locus_tag="KRH39_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBG8513253.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1872..2417
                     /locus_tag="KRH39_003377"
     CDS             1872..2417
                     /locus_tag="KRH39_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513254.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            2404..2577
                     /locus_tag="KRH39_003378"
     CDS             2404..2577
                     /locus_tag="KRH39_003378"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513255.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLLGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            2852..4777
                     /locus_tag="KRH39_003379"
     CDS             2852..4777
                     /locus_tag="KRH39_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG8513256.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            5178..7082
                     /locus_tag="KRH39_003380"
     CDS             5178..7082
                     /locus_tag="KRH39_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG8513257.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAESRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFKEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3012 a          717 c         1344 g         2065 t
ORIGIN      
        1 tattagaata taatataaga tttgttgagt agtatagtat tatattagaa tacaatatag
       61 gatttgttat gtagaatata taaagtcatg tagaatatac ctatataaaa ggattttggg
      121 tataaattta aaaattaata gcaaaatgag tattatagac atataattag tagtaggaag
      181 taattaaatt tttcctaaac aaaaaaaata gttgcaaata atttgattaa gtgatatagt
      241 aaataaggta agaataaaac aaagaagaag tccgcttctc accttacgcc agaggttagt
      301 aaggtaaata taagttaaag cttaatttta atttaagttt gtttaaattt ggtaacttat
      361 attgcggatg attacgtcat ccgcttttta ttttataaaa attttggagg tgtcctacaa
      421 ttagcaaaga attagcaatc aatgaacaaa taaaagataa ggaaataaga gtcctttcgc
      481 caacaggtga acaacttgga gtaatgccta ctaaagaagc tcaagcaatg gctaatagca
      541 aaaacttaga tttggttcaa atctcgccaa atgcaaatcc accagtatgt aagataatgg
      601 attatggaaa gtttagatat gagcaagcga gaaaagagaa agaagctaag aaaaaacaaa
      661 aaactatagt tgtaaaggaa gtaagactta gaccaggtat tgaacaaaat gatttaaata
      721 cgaaagctaa taatgctata aagttcctta aaaaagggga taaagtcaaa gttgaactta
      781 gatttagagg aagagaatta ggacataaag acataggtaa agaagttatg cttaagtttt
      841 tagatataat aaaagagttt ggagaaccaa caaaagcccc tgcatttgag ggtaataata
      901 tggttgtaat tatagatcca aaaaaataga tgattaactg agaggaggaa tttgtcatgc
      961 ctaaaatgaa aactcataga ggtgcagcaa agagattaaa gaaaacagga actggaaagt
     1021 taaagagagc taaagctttt aaaaaacata tattaactaa aaaatctgca aaaactaaaa
     1081 tgaacttaag aaaatcaact ttagtaagtg atggagacgc taagagaata gcacaattat
     1141 taccatacta agatacagat tataataata actatagaag agggatataa aaggaggtca
     1201 taacgatggc aagagttaaa aaagctatga acgctcgtaa aaagcataag aaaatattaa
     1261 aacttgcaaa aggattcaga ggatctagaa gcaaattata tagaccagca aatacattcg
     1321 taatgaaagc attaaaaaat gcttatatag gaagaaagtt aaagaagaga gacttcagaa
     1381 aactttggat acaaagaata aatgcagctg ctagaatgaa tggaatatct tattcaagat
     1441 taatgaatgg attaaaatta tctggtgttg aagttaacag aaaaatgtta tctgaaatgg
     1501 ctatacaaga cccagaagga tttgcaaaat tagcagaagt tgctaaagca aaattagctt
     1561 agtattgaat catgagatgt aaagtctttg agttatataa ataattcaag gactttttta
     1621 ttttttgaaa aatacttatt agtgttatgt acaaccaatt ttataatgat aaataaaata
     1681 agcaatacaa tagaatttta tgagatttat tataagaatt gttacaatta tttatgatag
     1741 ctataaaaag tcatagtaaa aatgatattg atgtatgttg atatatgtat aaagctttca
     1801 atcaatcttg aattaatatt atcaacatga taatattatc ttattaataa tataaataag
     1861 ggggagataa tatgaatgaa aaactaatgg attgttttgt aaacccaatt aaatgcaagt
     1921 tattgattga aattttttca aataatcgaa ctacagctaa acaattagga gaaaaatttc
     1981 aagatatatc aaaggcaaca ctttatcgtt accttaataa aatgttgaaa gatgatatta
     2041 taaaagtagt tgaagaaaat aaaataaggg gaactgttga aaaagtatat gctatgaagt
     2101 tagatatagc tgatgatgtg aacaaaatta tagatgagaa ctctggagaa gaatatgctc
     2161 aactgtttac tcaatatatg atggggtttt taagagaatt ttatgaatac gctgaaagaa
     2221 aggaaaagga tatcctaaaa gatggttcag gattttctgt ctgtccagta tatgctagta
     2281 ttgatgagat agttgaagtg agtaaaaaaa ttgctagtat actagaacct ctaagaaata
     2341 actcgcacga agaaggcaga agaatgcatt ctataggagt gataatcact ccacctaaag
     2401 aggatgaagg gaaataaatc tgatttacat gaggtagagc caatttacac ggaaaatcgt
     2461 tttatgattt acatatgtat ccagagcaaa gtatctctgc ttggattgta cttcattgta
     2521 ccatttcatc caatgacttg gagtggtaca atgaagtttt ttaatttttc atattaatgc
     2581 taaagaaatg aactttagac cacatatgca attaggttgc attatacaat tagaccacat
     2641 atacaaaata aaaatcagta tttttatagc agataaattg actgttattc ttcttaaaaa
     2701 tcatctatta gttattgtta gcatattgaa agaaattata gtaatctatt gaataaatga
     2761 atattgccca taatcatata aactgctata gtttaaaata aagcaggacg ttaaatttta
     2821 tagtaatcta aaaatttaag agggggattt tatgaaaata aaatttgtta ttatgccatt
     2881 ggtgatattt ttaatggtat tatttgtggg gtgtcaaagt acaaaagata cagaagttaa
     2941 agcaaaagat acagaaatta aaatattagg gacaggtgat ttacattcaa ttttgacaga
     3001 ctctatggtt tcatatgtaa atgaggaaag ggaaaagaat aaaaatctgc ttatggtaga
     3061 tgcgggagat ttctatggta cacaaagtag agaaatgtgg gaatggtctt ctggaaaaaa
     3121 gttaattaat attaaaagaa atggtagagc agagtatgat gacataatta agagtagtaa
     3181 agatgaagta cctatagtta aagatatggc aaagttgaaa tatgatgctg tagttttggg
     3241 agataatgaa tttgtttcaa atgataagca aagtcttgat aaactggttt ctgattttaa
     3301 aaataataat atgccattgg tatcagcaaa tatatatgaa caatctggag aaaattatgt
     3361 acaaccatat gtaatgaaaa atattaagac agatgagggc aatgtaaaag ttggaatact
     3421 aggtcttact ataaaagaag taggggaaac ccttggtctt gaggattttg aaaaggataa
     3481 aaaagctaga gagcttggag aacaagctga ttacaaagga aaattatatg caaatgattt
     3541 agtagaagat gcaaagaaat ggataaaagt tatggaaaaa gaaagtccag acataatagt
     3601 tgcagttgta cactcagggg aagaacctaa aacagataga aatccaggaa atagaataaa
     3661 agagttagca actacagtgg atggaataga tgcaattgta gctggtcata ctcatgaaaa
     3721 aatagatgaa cacaaatata aaaataaatc tggagatgaa gtaattgtta cacaaccagg
     3781 ttggcatgga gatagagtat cagagattaa tttcaaatta aataaagaga atggaaagtg
     3841 ggacataaag gaaaagtcta gtaaattaaa aataattgat aaagaagtaa gtatagttgc
     3901 tactgcagat ttacattcac atttctctga taaattaaca gttgaccttg ttaatgaacg
     3961 tagtaaccca attgaacctg ttgtagtaga tgcaggcgac ttcttagacc ctcaaacaga
     4021 tgaaatgact caatggtata aagagtggaa aagtataaga gataataata gtgataaaac
     4081 gattagtaga tgcccaatgg tcatggatat gaatcaggga atgtatgatg cagtagtact
     4141 tggaaatcat gaatttgtat ccgaaaaaga tgaattttgg tcagatgaac gccttttaga
     4201 tgctgttata gaggattttg aacaggtggc aatttctgtg ttatcggcaa atatatatag
     4261 gcaatctggt aaaaattatg ttaaaccata tattataaaa gatgtggaga cgagtgaagg
     4321 taatgtaaaa gtaggaatat tgggacttac tataaaagaa gtaggtaaag gtcttaaaaa
     4381 tgtaaactta caagagcaat ttcaatataa ggataaattg tatgcaaatg atttagtgga
     4441 agatgcaaag aaatgggtaa aagttatgaa aaagaaaaaa ccagatataa tagttgcagt
     4501 tgtacattca ggagaagaac caaaaaatcc aaaacatcca ggaaatagaa taaaagaact
     4561 agcaactaca gtagatggaa tagatgcaat tgttgctagt catactcatg aaaaaataga
     4621 tgagcatgaa tataaaaata agtctggaga aaaagttata gtgacacaac caggtgagca
     4681 tggtaaatat tattctaaaa ttacctttaa agtaaataaa gaaaagggtt cgtggattat
     4741 taatgataaa ttcagtaaaa caattgaggt tgaataactt tataaaattt atagataaag
     4801 taaattatct gatattgaca tgatatataa gataaagttt ctaatattag tataatgcat
     4861 aatgtgaaat attttaataa taagagaata aacaagacaa ataaaaatat tttaacatgt
     4921 aagatatagt atgaactatt atactaatgc ttactttata atttgaacat attagaataa
     4981 gtcttatttg aaaattttag tgtattagag taagtattat tttaccagta aaaaatattg
     5041 agaactatat gtagatttgc ttaaaaagga atttacatat agttcttttc gtataataaa
     5101 tagtacaatc ttatatggta aagataaagt gtgatattaa attttattat catccaaaat
     5161 ctaaggagga gtctcttatg aaaataaaat ttattatagt agcatcaata atatttttaa
     5221 tagtattact tgtggggtgt caaagcacta aagatacaga aatcaatgta ctcgccacaa
     5281 gtgatttaca ttcaattatt ccagataatt taatttccta tgtagaagag gagagaaaat
     5341 atgatgagaa tctacttatg gtagatgcag gagatttttt tgacatgcaa agtggtgaga
     5401 tgaataagtg gtttactggt caaaaattag taagatttaa agatgggata ccagagtaca
     5461 aaaaaatagc tgaatcacgt gaaggagaag ttccattggc taaaaaaatg gcaaagctga
     5521 agtatgatgc agttacattt gggaatcatg aatttgtatc taatgacaag cagagccttg
     5581 ataaattggt ttctgatttt aaaaataaca atataccatt agtatcagca aacgtatatg
     5641 aacaatctgg agaaaactac gtaaagccat atgtaatgaa aaatataaat actgacaagg
     5701 gaactgtaaa ggttggtata cttggtctga ctataaaaga agtaggggaa cgttcaaggt
     5761 ttaaaagttt tgaacaagat aaaaaggcta gagaacttgg ggaacaacct caatacaaag
     5821 ggaaattata tgcaaatgat ttagtagaag atgcaaataa gtgggtaaag gttatggaaa
     5881 aaaaagaaaa gccagatata atagttgcca ttgtacactc aggtgaagag ccaaaaaatc
     5941 ctaaaaatcc aggaaataga ataaaagaac tagcaacaac tgtagaggga atagatgcta
     6001 ttgtagcagg tcatacacat gaaaaaatag aacagcatac atataaaaat aattctggtg
     6061 aagaagtaat agtaacacag cctggagctc atggtaatag tatatcaaag attaatttta
     6121 agttggataa gaagaatgat aagtggttta taaaagacaa atatagtgaa ttaaaggttt
     6181 ttggaaagga agtaaatata attacaactt ctggtctgaa tccaaaattt tcagatgaaa
     6241 tggcagttaa tttgtttaat gaaagagata gagatgttca acctatcttt atagatactg
     6301 gagatttttt aaatactaat acaaaagaaa tgactaagtg ggctaaagag tggcaataca
     6361 atgaaaataa aggcatctat aaaaaaataa atgaatggcc aattgtatat cttggttatg
     6421 atgctgtagt acttggtagc catgaattta aagaggataa agagactttt gaggaaaaaa
     6481 aatctacatt ggattatatt atagaaaact ttgaagaaat gaagatacca gtattatcag
     6541 ctaatatata tgatgaatct ggagaaaatt atgtaaaacc ttatataatt cataatgtag
     6601 aaactaggga aggaaatata aaggttggag tattaggtct tactactact gataaggatg
     6661 aagaagactt aagtagctca agcttaagca aagactcaaa gacttcgaaa attcctgaag
     6721 aaacaggaaa gttatatact aacaatctag taaaagatgc aagcgaatgg gtaaaagtta
     6781 tgcaaaagga aagtcctgat gtgattgttg cagttgtaca ttcagataat aatagtactc
     6841 taaaaaatcc aagtagtgaa gtcaaaaaac ttgcaatgtc agtagatgga atagatgcta
     6901 ttgttgtagg tcatacgaaa aataaggtag aagaacatat ttacaaaaat aagtctgggg
     6961 atgaggttat agtaacacaa tctggagagg attgttattc aaaaataagt cttgaactaa
     7021 gaaaagaaaa tggcaagtgg aatatcataa acaaatatag taaagcaata aaaatgaagt
     7081 gaatttattg atatataaac cattattaat actgattttt ataatataat aaatactg
//
LOCUS       DAEORZ010000176         7104 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEORZ010000176 DAEORZ010000000
VERSION     DAEORZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.180"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            135..3314
                     /locus_tag="KRH39_003381"
     CDS             135..3314
                     /locus_tag="KRH39_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG8513258.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKASLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYDPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEFHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQVLSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPTNLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPIALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIIDLFN"
     gene            complement(3599..4363)
                     /locus_tag="KRH39_003382"
     CDS             complement(3599..4363)
                     /locus_tag="KRH39_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8513259.1"
                     /translation="MAYFNYNNKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEKMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLEPFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVFLTGSREDEFVSLISNDFFENTFSSLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4602..6095)
                     /locus_tag="KRH39_003383"
     CDS             complement(4602..6095)
                     /locus_tag="KRH39_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG8513260.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLEYNIPSRVQREVIPKLLKGQNLV
                     VKSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKLRC
                     SAIFGKQPIKEQIAELKQRVHIVVATPGRILDHINKGTIKLENIKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMPDYSVINIEENESDTNQRTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEQSRITHGKILFEEYSKG
                     ILKKVSKRKKADKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYQEIPIKKKMVKVKRDIR"
     gene            complement(6423..6965)
                     /locus_tag="KRH39_003384"
     CDS             complement(6423..6965)
                     /locus_tag="KRH39_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8513261.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGN
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT         2604 a          998 c          800 g         2702 t
ORIGIN      
        1 tagtaaattg ttatttacac atatattgta atagataaaa gctattcata tctgccttaa
       61 ttttagtctt aaatttagag tgtaaattga atgcatttag attataatat atacagtgag
      121 gtaaggtgaa taatatgaaa atagatatga tatataaact aataagaaga aatacagaac
      181 catcaagatg gtcaagaggt gtaaaatatt atactaatca actagttgat tatatagaca
      241 tagatattga tgacctacaa aataatctgt tgacatttcg tggtggagtc aattcagaag
      301 ataattatag cttttatttt aattcaataa ctatagatat aaagcacttt aagataattg
      361 aaaatgattg tagttgtgct gactgtcaat caaagggcaa aagtattttt atatgtaagc
      421 atatagttgc tattgtgata aaaagcttaa aacaaataaa aaataaaaaa gatgttcctc
      481 ctattctaat agatatatta ggtgcagata aggataagct ttctaataca aattctaaag
      541 ccagcttaga tttaagcaag caattacttt ctttgttaga caatactaaa agagaaaggg
      601 ttaatttaga tgtattgata aatgtggatt caaataaagc aattgctgaa tttaaaatag
      661 gaattgataa aatgtacatt ttaaaaaaca ttactgaatt tgcatattca aaacttaatg
      721 gatatgaatt agattttggg aagaaattta cttacgaccc caacattcat tattttgctg
      781 atgaagatga gaagttagta gatattatag aagaatatgg gacaaatctt tcatacaact
      841 tttcttcatc acactcaaaa aaatttatgg agataaatgc aagctcttta aaacaatttt
      901 taaatgctat taagtacaag gattttaccc ttattttttc taaagaggaa tttcatccaa
      961 agatattaaa tgatactcta cctgtaaggt ttgattttaa aaatatagaa gataaaatag
     1021 tcttatattc taaagattca ctacctgttc cacttactaa aaaaaatgat gtacttttat
     1081 acgatggcaa tatctattta ttatcacata aaaaagctca tgattattca aaaatatatc
     1141 aagtattatc aaagactaaa gagttaaaat ttgataaaga ggattctaag gatgttttag
     1201 gattgctagt tcctagatta aaatctatat ctcaagaagt tcatttagat gataacataa
     1261 aaaataatat aacaaaagat tttaaatctg agttctactt tgatatgatt gatggcaaca
     1321 tatgttgtga tgtaaagtat atatatgatg atgaagataa aaaatttgtg ctccccaaca
     1381 tacaaaagga agctactatt gaaaataaac tgacttcttc acaatttaca aaagaaaata
     1441 atcattatgt gtttaaagga actgaccatg acctatttac atttttagat ttacaattag
     1501 aatctctaaa ggaatttgga gatatatatt attctgagaa gtttaaattg aaaaaaatat
     1561 ataacgcttc atcaattcaa gcttcaatca atcagactaa ccagaattac ctagaattta
     1621 attttaatat atctgatata aatccaaaag agtacaagga tattttgaaa gcttttcaag
     1681 aaaaacgtac tttttacaaa cttaaagatg gaaactttat agatttatca gaaagggaaa
     1741 caaaagattt ttttgaatta gttgaaaatc tagatataat ggataaatca aaaaataata
     1801 aaatccataa aaataaagct ctatatataa acgatgtaat acgtagtaaa aatttaaagt
     1861 ttatagatgg taaaaaagaa ctagatagta tatgtgaaaa atttagaaac tttgatagca
     1921 taaatttaga aattcccaca aatttaaatg caaaccttcg tgactatcaa ataaatggtc
     1981 tcaattggtt taaagtttta gattattata aatttggtgg tatattagct gatgaaatgg
     2041 gtcttggtaa aacaatacag actattgcat ttttattatc attatctaat aaaaaaagtc
     2101 ttattgttac accgacatcg ctcatttata attggaaaaa tgaatttgaa aagtttgctc
     2161 cagatataaa agttttattg atacatggaa ataaaagaga tagagaaaaa tgttttatgg
     2221 aattagaaaa ttttgatgta atacttacaa catacggtac tcttagaaat gatttagaaa
     2281 aatatagtga aataaagttt gattattgta tattagatga agctcaaaat ataaaaaatc
     2341 caatagcttt agtaactgag tctgtaaaaa gtataaatgc agaaaataaa tttgcactca
     2401 ctggtacacc tatggaaaat aatctactag aattatggtc tatatttgat tttataatgc
     2461 ctggctatct atatagtaaa gcaaagtttc aggaattgtt tataaataaa gaagataatg
     2521 ttaaaaactt gaaaaaactg ataaaacctt ttattcttag aagaagtaaa aaacaagtta
     2581 tgaaagaact tccagataag attgaaaaaa acttttttgt tgaattgaat aaagagcaga
     2641 aaaagatata tagtgtatat tcaaaagata tacaagataa aatgaaagat aaaaatctaa
     2701 agaaagataa aatagttata ttttcttatt taactaaact aagacaactt tgtttagacc
     2761 caagtatagt agtaaaagat tacaataaaa aaagttcaaa aattgaaacg tgtttagaaa
     2821 tacttaggga tagtataaac gaaaaccaca aaatattatt gttttcacaa tttacttctg
     2881 tgcttaagaa catcagtaag gaacttgata agtataaaat taaataccat tatatagatg
     2941 gtaaaactaa tgctaaagaa agacttgaac ttgtagatga gttcaataat agtatggata
     3001 aaaaagtttt tttaatatct ttaaaagcag gcggaactgg tttaaatctt acatctgctg
     3061 atatggtaat tcactttgac ccatggtgga atccatcagt agaaaatcaa gctagtgata
     3121 gagctcatag atttggtcaa aaaaattcag tacaagttat aaaactgatt gctaaaggga
     3181 ctatagaaga aaaaataata aaattacaag aaagtaaaaa agagcttata aatcaattta
     3241 taaatggaga actttctaat gaaggtgtat taaaaagtct atctgatgag gagataatag
     3301 acttatttaa ctaaattatt taatcctaca gcttaactat ataaatttgt tctaacattt
     3361 atatagttaa gctacaatac ctgaataaat aaaatttcat attgttattt aatcccaact
     3421 ttacaatcta atgatatctc tttctataca tattgcatat acaaatgtaa taacttgact
     3481 attagaatat tttgttttaa caatcctatt tcatcataat aatgaagtat atgtacactc
     3541 acttttgtaa gttttacaat ctattttaca gttttcatta ttgtaaattt tgattaaatt
     3601 attcctctaa aaatttcttt gctacatttg aaaaatctaa tccattcgat aaaattgcag
     3661 gatgaccacc tttatcaaac aaatacattt taccattttt aatcttttca agaagtgatg
     3721 aaaaagtatt ttcaaaaaaa tcattagata ttagagatac aaactcatct tctctacttc
     3781 ctgttaaaaa cacctctggt tgcatagttt ctagaggctt atgaaaaaat tttcctatag
     3841 ttttataatg ttcaaaaata gcttctgtat cattatctac aactttttcc cagttttctc
     3901 cttgattata aatataaaaa gcttttgcac catcatcttg ctttgatgct tcacgctcta
     3961 ttttaacatt ttgtacaaat ggctctaaag ggacttctcc ttcaaagcta tccgcaatta
     4021 ccttattcac taaatcaggt ctttcaagtg caacatttaa tgcaacaaga gctcctccac
     4081 tactaccaat tatgtttact tttttataat tcattttttc taaaaaacat atcacttgca
     4141 tagcttcatc aaaccataag tcagcagaaa acttatctac catttgagat tttccatgac
     4201 ctacaaaatc aattaatatt actttatatt catctttata aaaatcgatt atatcattaa
     4261 acatcttaga agatgctgta tttccatgta aaaaaagtaa tggtgtacca tccccaattt
     4321 cattatagta gcactgtttg ttgttatagt taaaatatgc catatatttt ccccctttaa
     4381 tatccctata agtatatatt tattacttat atttaaaatg actattgtca taaacactta
     4441 aaaatagtat agcttagaat gtagataaaa tcaacattcc tttccttaac aacttctatt
     4501 gtttttttgc aacttcattt cgataacaga ataattcatc aatattgata cttttactta
     4561 aatatatgat ttcatcatca gagagagcag taataactaa attatcgtat atctcttttc
     4621 actttgacca tttttttctt tataggtatc tcttggtatt tttttaatat caaatctccc
     4681 ttataattta atatatctac atatgaacat aaatcttgaa cttcaattac acctatatca
     4741 tcattagtta taccttttat attactaaat gcaccaacta tatctaaaac tcttattttc
     4801 ttctttttgc cagcatttaa atataattta acaacttcac tttctatatt tagttttata
     4861 tctttacttt tgtaagaatt tttatcggct ttttttctct ttgatacttt ttttaggatt
     4921 ccttttgaat attcttcaaa caaaattttg ccatgtgtaa ttctactttg ttctatatct
     4981 tttaattcat ttattttata tcctatatac gtttctatat tctctatata tttttcatcc
     5041 ttttcagtta ctatagtaat tgcctttcca ctatttcctt ttctcccagt tctacctatt
     5101 ctatgtatat agttttcttt atcttgtggc acatcataat ttattactaa agaaatatca
     5161 tcaatatgta ttcctctgga cgctacatca ctactaacta atatattaaa tttttggttt
     5221 ttaaaatcct taattacaaa aattcttctt tcttgcgata aatctgcatg taactctctt
     5281 attaaaaaac cttctttact catcttatta tataatttag atactttctc tttagtatta
     5341 caaaatatta tcacactctt tggattttcg ctgtatatta attcttttaa aaggatatac
     5401 ttctctcttc catttgcctt gattattttg tcatctattt gtcttgttct ttgattagta
     5461 tcgctctcat tttcctctat atttataaca ctgtaatctg gcatatattt ttcacatata
     5521 tattttatct cttcatctat tgtagctgaa aaaagactta ctattttctc cttaggtaaa
     5581 tttaataata ttttttccat ttgttcaaca aaacctttat taaacatttt atctgcctca
     5641 tctattacta aatactttat attttccaac ttaatagtac ctttatttat atggtcaagt
     5701 attctgcctg gagtagctac aacaatatga accctttgtt tcaattcagc tatttgttct
     5761 tttataggtt gtttgccaaa tatagcacta catctcaact tctttagtct tcctatatct
     5821 gatatctcat cttttacttg caaggctaat tctcttgttg gaaccactat aagagcttga
     5881 atattattgt aatctacatt tatattctcg cataaaggta ttgcaaaact tgctgtcttt
     5941 ccacttcctg tttttgactt aactactaag ttttgacctt ttaataattt tggaataact
     6001 tctctttgta ctctagaagg tatattatat tctaaactct tcaatgactt aagtatcttt
     6061 tcatttaact taaatttttc aaatgtatat ttcatatatt accaccttat tatatttatc
     6121 ttatctatat atttttctac attatattat tataatacaa ggttctacta tttttatatt
     6181 atttattaga ctgaagataa aaatatatac tatataaacc tatagtttag tagcaaatta
     6241 attaaatttt tatatttaaa tacctccttg aatatactga taaatcaata ttttcaagga
     6301 ggtatctctt gtcaataatc aaactaatct atcttttgaa agtttaaatt atactaatac
     6361 attgtatttt tatttatatc taatattaaa aggtattaat caatacctaa atcaccataa
     6421 tctcacatat ttatatcttt attcaatata ccttctgtaa tcaatcgtgc tatataatca
     6481 taacctgatt catttgcttt gttacaatca ttcacattat cacagaaaaa actttcaatc
     6541 tgtattgcaa ctggatttgt atttctcaat atatataaat tttctctttt tttagctcct
     6601 ctattttcaa ataattctcc aagtctatta gatactcttt tagcatattc taatccttca
     6661 ttgccatagt aaagcacttc tgtaccacat tgtgttccat cagctttatt taaatgtagc
     6721 tctactaata aatcgtattt accactgttt acttttggaa ctctatagaa aaattcttct
     6781 ctttctgaca taaagtgtct ttctggacaa attattacat ctactgtatt accttgactt
     6841 ataagcatct cagctaacat aggagctaat tttttgttat aattgtattc atcaactata
     6901 ccatttacac ctgtactttt accacctttt agtatactat gacctactgt tatgcatatt
     6961 ttcatttact tttttcacct tcttttaagc caatttttat ataatagtaa tataatttta
     7021 aagttattac taataataat atattaattg aagaaggtaa aagttctccg tgattatcat
     7081 tcttttaaca tatttgtctt attt
//
LOCUS       DAEORZ010000177         7050 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEORZ010000177 DAEORZ010000000
VERSION     DAEORZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.181"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(19..684)
                     /locus_tag="KRH39_003385"
     CDS             complement(19..684)
                     /locus_tag="KRH39_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG8513262.1"
                     /translation="MKKDTSHIIKRAEHFQVILIGEGLMVGAVAGLIVLLYRILLGQA
                     GTWLSQILKFVEDSTIKIIAWFAVLAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKI
                     KQVWWRVLPTKFIGGFLSLMGGLALGREGPSIQLGAMAGKGISRALDRGKTEEKFLLT
                     CGASAGLSAAFHAPLAGVMFSLEEVHKNFSVSVLISVMTASLTADYISANFLGMESVF
                     QFD"
     gene            complement(844..1380)
                     /locus_tag="KRH39_003386"
     CDS             complement(844..1380)
                     /locus_tag="KRH39_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG8513263.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1453..1866)
                     /locus_tag="KRH39_003387"
     CDS             complement(1453..1866)
                     /locus_tag="KRH39_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG8513264.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2359..2997
                     /locus_tag="KRH39_003388"
     CDS             2359..2997
                     /locus_tag="KRH39_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBG8513265.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEGTI
                     SDMQSNINGIEGTISSMQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISS
                     MDGTINSMQDSITDMQVEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIK
                     SDVKINRNTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3605..3784)
                     /locus_tag="KRH39_003389"
     CDS             complement(3605..3784)
                     /locus_tag="KRH39_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG8513266.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(4010..4543)
                     /locus_tag="KRH39_003390"
     CDS             complement(4010..4543)
                     /locus_tag="KRH39_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG8513267.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            4860..5210
                     /locus_tag="KRH39_003391"
     CDS             4860..5210
                     /locus_tag="KRH39_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513268.1"
                     /translation="MGKVRIGCKKEKYFYVRNPVKSLVDRITENEQFAIYHFTHKYNW
                     RNKVFSILQRLIRLEKSKNELYMYEALVSVSFLWLIMIKNIVLPPIKHKNIINIHMQK
                     ILRYIEKYYCFTLY"
     gene            complement(5881..6453)
                     /locus_tag="KRH39_003392"
     CDS             complement(5881..6453)
                     /locus_tag="KRH39_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG8513269.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(6515..6778)
                     /locus_tag="KRH39_003393"
     CDS             complement(6515..6778)
                     /locus_tag="KRH39_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG8513270.1"
                     /translation="MSLNRRELLERILEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(6778..6999)
                     /locus_tag="KRH39_003394"
     CDS             complement(6778..6999)
                     /locus_tag="KRH39_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG8513271.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         2503 a         1201 c          860 g         2486 t
ORIGIN      
        1 agtgttcgta gctatttttt aatcaaactg gaatacagac tccattccta ggaaattggc
       61 tgaaatatag tctgctgtca aagatgctgt catcacagaa attaaaacag atactgaaaa
      121 gtttttatgg acttcttcaa gtgaaaacat aacacctgct agtggtgcat gaaaggctgc
      181 tgataatcca gcacttgccc cacaggttaa caggaatttt tcctctgtct tccctcggtc
      241 aagagctcta gaaatacctt tacctgccat agcaccaagc tgaatggaag gaccttccct
      301 tcccaaagca agaccaccca tgagtgataa gaatcctcca ataaattttg ttggcaacac
      361 tcgccaccat acttgtttta ttttccctgt catttccccc tcaagctgag ggataccact
      421 tccagaaatc attggctccc agtccaccaa ctttgataca atccacgcaa gtactgcaag
      481 tactgcaaac caagctatta tttttattgt agaatcttct acaaatttta aaatttggct
      541 aagccaagta ccagcctgcc ctaaaagtat cctgtataaa agcacaatca aaccagccac
      601 agccccaacc ataagtcctt caccaatcaa aattacctga aagtgctctg cccgtttgat
      661 aatatgagat gtatcctttt tcatactgtt cctccttcat ccaataattc tacacaatct
      721 gaatctccaa tgactaaagt catgagtgtg tacagatatt ttttaatcaa caaaaataga
      781 tttttattta ctaaatataa gaaaataatt gctcaatttc tcaataaatg taaatttatt
      841 cttttaggta tttattctcc acagtcgtgc ttcttccagc aaaatattta ttagttcttg
      901 ctttctctgt gtatagattt tacgattgtc ctcaaactgt aatgctaatt tttgtttatg
      961 actatcataa agttttgctt tgtctgtaaa agtatttaga taatctcgaa aattgatata
     1021 attattccat gcaacactat tccattcaac aacatgaata tggtgagtac gagtattttt
     1081 ctcaaaatct cccatagcaa aaagtaattg ttttggtaca tcctgacctc gaaaaacgat
     1141 tccacattgc tctaaccttt cgaggtataa tttagcttcc tctaaagaag caacacctac
     1201 aacaatatca ataataggct ttgcgctaat actgggaata gaagtgcttc caatgtgttg
     1261 aatatcaatg gcagtcttat cccatatact tttcaataat ataatgacat cttttgcact
     1321 tttatcccat atcatctgat gttccaataa tttaaccata ccacatttta aacctatcac
     1381 tatataccac ctcaatataa tccaccttta tatatattac aattttttct ctcttgataa
     1441 acagtgattt agctatttca ctttttcttg acactcgcta cattccctat catgaataga
     1501 tataattcct ccacatttag aacaagtata tctctccttc tgcttttgca taaacttctc
     1561 taaaccgtac tgctgaacaa atttactatt ttccataagg cttgcttgat atctcttgtt
     1621 ataacttttt tcaagatttt ttatgagttt acaaggaaaa ttgaaacatt caaaacaata
     1681 aaacaaccct ttttgacaga cacaatcctt tattttacac ttacggcagt gttctggctt
     1741 tcctgtatcg ccgttcaaac aaccagcaca tggctttttg tgataacaat gcttatagca
     1801 aaccatacaa ttcattccac aaggagcaaa cataatcgta tcaatactct cttttggcat
     1861 tttcataaca ttttactcct ttcaaaaatc caatttgtcg aattgttata tattcatatt
     1921 gtaacataaa aaatagctaa aaaattcatg cactttcatt gccaataaat tttcaaggaa
     1981 atatgaaggt ttaaaactaa gaataaaaag agataaaaag gaagctacta ataaaaatat
     2041 tcaaatacaa gtaatcaata taaataactt aaggtatatt taaaagtata tgacattacc
     2101 agcagtttta aatacaaata taaccacaca tgcctcaaaa acacttatta cagtatctta
     2161 gaaacttaga caatacataa gattcattaa taaaaaagtt ctcaatatag tcattaataa
     2221 aaacgttctc aatatagata tatttaacca tgttttgaat agcagataat tatatgatta
     2281 aaaattaaat atgacatagc gtttgtggaa tataacacct aaattatgtt ataataataa
     2341 aacaggagtg tgatagtgat ggatgaaaaa atattagagt tattacagga aatgcaaggt
     2401 aatattagta atatgcaagg tagtatcagt ggtatcgaag gcactattag cgatatgcaa
     2461 agcaatatca atggtatcga aggcactatt agcgatatgc aaagcaatat caatggtatc
     2521 gaaggcacta ttagtagtat gcaaggcaat atcaatggta tcgaaaacac tattagtggt
     2581 atgcaaggca ctattggcga tatgcaaggt agtatcagtg gtatgaatgg cactattagc
     2641 gatatgcaag gcagtatcag tagtatggat ggcactatta atagtatgca agacagtatt
     2701 actgatatgc aagttgaaat taaagaagtc aatagtaggt tagacaatat ggaacctgat
     2761 attaaagact taaaaactgg acaagttgaa actagtagta gacttgatac tattcagagt
     2821 gatgttacag aaattaagtc agatgtaaaa attaacagaa atacattaga tacagtgatt
     2881 aaacatacct cttcattagt aaaagataca tcagatatta aaaatgattt aaataatata
     2941 gagatagtca catctaaaaa ttcgaatgat attgctaaat taaaatcagt taaataatat
     3001 actatagtaa aaaatacctg aaaatttcac atacttcttt aaagttatac caaattttat
     3061 acagaaatac tgattatccg acaacacact ttacttataa ataatttatt aaaaaaatac
     3121 tcattttatc ctaaaattaa cacgactaaa tgagtcttga tataataata aaaaataagg
     3181 gagattgaac actaaaaagt tgtgtatctc ccttatattc tataatatag ttatataatt
     3241 tttcagccta aaataaaagt ccatctttga ttaaaacaca tccccttatc acttataaaa
     3301 ggataaaatc ttgttttcat ttttccattt atatcttttt gcttaagagt aataattttt
     3361 tcttttgata ctttaagtgt ttggttaaat ttaaccaatc gactaagttt tgttcaacat
     3421 ctatttttca tattttccaa tacttaatct attttttata aataattctt tcatttctaa
     3481 ctcacttaca ttttttacat aaaacgaagc atataaattt caaccttatc ttgtatggtt
     3541 cttaatactt aatgagtagc ttaagtatca aacattataa attttcccct tacatttaac
     3601 actcttacca tttccaaaat atacaaaata tattttgaac atttgtacaa tgatgaaata
     3661 caatacatga aaatacaata tcataactgt tgtctaaaaa tagaagttct tcaacataac
     3721 ctttcacaaa aaacatatta tctaaatatt ttttgtctgc tccttgtttt cctgtttgta
     3781 gcatataaat tactgcatct aaacatataa ctactcacac gaaaaatgaa aataaacttc
     3841 caaaatacaa gctctaaaaa ctacctctaa agttgtataa tgtttactag gaataaggtt
     3901 gtataacata tagtttaaat aatcattttt taaaattgca agttattgct ttcaaaatta
     3961 gatgtttgta tatcaaataa atgattaaga atttctaaat ttaatggctt tataatgcac
     4021 tccctttctt tgggataaaa taatgttgca cagatacttt ttctaaagat aacaatttta
     4081 tttttctatc aattccacca gcatacccag tcaaactccc atttgttcct acaacacgat
     4141 gacatggaat aataatagag attccattat gccctactgc accaccgatt gcttgtgctg
     4201 acattttaga tattcctttt tgttgagcaa ctttatatgc aatttctcca taagtgattg
     4261 ttttaccata tggtatttgt tgtaaaattt tccacactgt agtctgaaaa ggagtaccta
     4321 ccatatggag tgatggtgta aaattgggtt cttttcccga aaaataaata tctaaccatt
     4381 tctttgtctg ctcgaaaatt ggcaattctt tttcttgatg ttcttggtca agattataag
     4441 cataatactt ttgattctca aaccaaagac cagttaaccc aatattatct gatgctaaaa
     4501 gaatatttcc aattggacta tggtaatagc taatatattg catacaattc ctccttatat
     4561 tctgaaaact tcactcaaat cattagacac ctcttttcta tctaccatta tataaaaaca
     4621 tactaatata atttccttct gactacactt tcgttataat atctatcctc atatcatatt
     4681 agttttatat ttacctgcat ctgttcatcc ttttttccct ttccctttga tgaatataaa
     4741 attcatacat ttaagcttcc tactgtatta acttttatag aagtgtatat tataatttta
     4801 tcaccaaata gaccaaaaaa atatcgatat aacatcaagt aatatcataa tactttcaaa
     4861 tgggaaaagt aagaataggc tgtaaaaaag aaaagtattt ttatgttaga aatccagtaa
     4921 aatcgttagt agatagaata acagaaaacg aacaatttgc tatttatcat tttacacata
     4981 aatataattg gcgcaataaa gtattttcta ttctacaacg attaataagg ttagaaaaaa
     5041 gtaaaaatga actgtatatg tatgaagcat tagtttctgt ttctttcctt tggcttatta
     5101 tgataaaaaa tattgttttg ccaccaataa aacacaaaaa tataattaat atccatatgc
     5161 aaaaaatttt acgttatatt gaaaaatact attgttttac attatattga agagtactat
     5221 tgtgaaaact taattttgga gtactatcaa gcagtgataa tatcaataaa tcagagtgtt
     5281 ccagaggatt tgaactaagt ttaaatacta taccctgtaa atattttact gagttttgtt
     5341 tattaaaagc aactcaattt ctaaaaggca caaatgagct aataggtgac attactataa
     5401 aagttgtttt tcatcaaata atctatttta gtaaatgttt taaagaaaaa cgggatattc
     5461 tccaaaagtg tacagaaata taaaaaaaga gatatgattt tttatttctt ttgattatcc
     5521 aaagagtaaa taaaaaatat tgtaaaaaac ttactaacaa cagcaatgct ttgaactatg
     5581 gtaatacttt gaacttttga ttggactttt tttgctaaaa taatatattt aaatagcaaa
     5641 gtaatctttt taatcaataa aaaataagag ataaaagaaa tcaactaaaa cctttacctc
     5701 ttatttttga tagtttatca gagctaatat ttacactctt tttatttttg atacttcatt
     5761 aaaaatgatg cttattctct tttcactttt aattcttcat tagaacttat gcttactctc
     5821 cattcacttt taatccttca ttagaactat gcttattctc cattcacttt taattcttca
     5881 ttaaaactga tgcttgcact ctgtaaactc ataaacatcc attaatgctt caccaaatct
     5941 ttgatagtga actacttccc tctcacccaa aaatctcaat atatctttca aatcagggtc
     6001 atcagttaaa tttattaatt gatagtacgt agccaatgct ttttgttcag cagccatatc
     6061 ctcatgtaaa tcagttaccg aatcgccaaa gacattaata taacttgtag taaagtttac
     6121 tccattagaa tctactggct gaagaccatg tccaaaatta gcataattac taccaagtcc
     6181 agcagcctta agctcttctg gagtacaatt gcctattaat tgatataaca ctgaagaaat
     6241 tatctcaaca tgagccattt cctctgtacc tatatcagtt aaaagtgcac gagattttcc
     6301 agttggcata gtatatcttt gttgaagata tctagttgca gctgccaact caccattagg
     6361 tcctcccaac tgagtcatga tatattttgc cattctaggg tctgcttgtc ttatgttaac
     6421 tggatgttcc agtgtttttt gataaatcca catattatct tactcctcct taatatatat
     6481 ttaataataa atctaatttt aaagtttaat aattttattt ataaaatgat ttgtcccatg
     6541 gccaaggttc agatatccat gaccaaggat attcacttga ttcatatcca aaattagtta
     6601 atgctccata tttagattca taatcacaca cagcttgtgt aaatttatcg cagtatctgt
     6661 tgtatgaatc aagagctttc ttatcccttg gattgttatc taaatataaa tttaattcta
     6721 tacaggcaaa ttgatattct aaaattctct ctaaaagttc tcttctattt aatgacatta
     6781 gtacatgtta cccccttttt tagctctaac tctattttta ggatttgcat atagccaatc
     6841 attatagtta tttgactcta ttaaatctaa ttcaggaaat aaagtacctc tttttaatgc
     6901 tgtttttgga ttgtatgtgt catcataaat ttgacgattg atgtatggtc tagcaaattc
     6961 acaacaatag tcttgtttat cctcttttaa atatcccatt aattatcctc cttaattttt
     7021 agaattttaa aatagcatta tttttaaaaa
//
LOCUS       DAEORZ010000178         6862 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEORZ010000178 DAEORZ010000000
VERSION     DAEORZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.182"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            209..610
                     /locus_tag="KRH39_003395"
     CDS             209..610
                     /locus_tag="KRH39_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBG8513272.1"
                     /translation="MEDSQYSKEYSEDSLFKKIKSVFSKAGVSVIYGVLLLFYSLKDP
                     SVPMGAKATIISALGYFISPIDLIADPIPVAGYGDDLTAIITALGVISVFITPETKRK
                     AREKTLEWFSDATEDDFNTIDGQIEKRNSQN"
     gene            727..972
                     /locus_tag="KRH39_003396"
     CDS             727..972
                     /locus_tag="KRH39_003396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513273.1"
                     /translation="MKIASILGIILIGALILLSLIGGFGLITWIGMMIVGTIGIIIGG
                     IFNIIGIFIAFGFRLLLPIIGIVLVIFIISKIIAVKK"
     gene            1925..3187
                     /gene="tetA(P)"
                     /locus_tag="KRH39_003397"
     CDS             1925..3187
                     /gene="tetA(P)"
                     /locus_tag="KRH39_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter TetA(P)"
                     /protein_id="HBG8513274.1"
                     /translation="MVNKLSAYKTYLLFSAITAMCFSLVATVMIVYHIETVHLNPLQL
                     ILVGTTLEVACFIFEIPTGIVADVYSRKLSIVIGVVLTGVGFILEGSISSFVFVLVAQ
                     IVWGLGSTFISGSVEAWIAEEEKDKDLDKIYIKGAQAGQIGSVIGIVLSTVIANLSVR
                     LPIIVSGVLFIILALFLWLYMPENNFKSSAPEDLNTFKKMGYTFKSGLKFIKNKPIII
                     ILLSVTLFYGLSSEGYDRLSNMHFLQDTMLPKLGNLKPVTWFGIFGIAGMILSAIVMH
                     FMEKKLKDDDKNKNGKLLLCINIFYISFMFIFAITKRFNLMLIAYLATSTFRTINEPI
                     FSAWLNGHIDDKARSTVLSINGQINSLGQILGGPIIGIIATNISVSMGIACTSLLVTP
                     VLVLYIVAMIMDKKVVDRVGGIDYEENN"
     gene            3171..5129
                     /gene="tetB(P)"
                     /locus_tag="KRH39_003398"
     CDS             3171..5129
                     /gene="tetB(P)"
                     /locus_tag="KRH39_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein TetB(P)"
                     /protein_id="HBG8513275.1"
                     /translation="MKKIINIGIVAHVDAGKTTITENLLYYSGAIKSVGRVDLGNTQT
                     DSMELERKRGITIKSSTISFNWNNVKVNIVDTPGHVDFISEVERSLSVLDGAILVISG
                     VEGIQSQTRILFETLKELNIPTIIFVNKLDRIGANFNKVFEDIKKNMSNKVVRLQEVY
                     DVGSKAVYIKKLFDTCMINDDAIDVLSDLDEAFLERYIGGIEPDKEEIQEKLSLYASE
                     GSLYPVFCGAAAIGLGVEDLLDGICSYFPFAGDDCESDLSGVVFKIERTSKNEKKVYV
                     RLFGGKISVRDKIQVPNKEIAEKVKKINRLENGGVVEAQRIEAGDIGILYGLTSFQVG
                     DVIGISNDKIKNISIAKPALKTTISAIDKEKNPELFKALTLLAEEDPLLELEMNDIDK
                     EIYVNLFGEVQMEILSSMLDDLYGIKVEFSNIETIYKETPKGFGASIMHMQEDLNPFW
                     ATVGLEIEPAGRGEGLRYISNVSVGSLPKSFQNAIEEAVIKTSKQGLFGWEVTDVKVT
                     LSCGEFFSPASTPADFRNVTPMVFMEALYKAQTVLLEPLHEFELKIPQNALSKAVWDL
                     ETMRATFDNPIVIGDEFSIKGLIPVENSKEYKMKIASYTEGRGMFVTKFYGYKEASAE
                     FSKARKKTTYDPLNKKEYLLHKLNAIRD"
     gene            5320..5598
                     /locus_tag="KRH39_003399"
     CDS             5320..5598
                     /locus_tag="KRH39_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513276.1"
                     /translation="MKIDFKITKDDYISFNLHHLENSKSQKSTFNILRYAVPIVLSIP
                     IYFTGTGIFNQPNIYWIIVAIVFLVIWILTYPKQYKKLVAKETDKLIS"
     gene            5614..5853
                     /locus_tag="KRH39_003400"
     CDS             5614..5853
                     /locus_tag="KRH39_003400"
                     /inference="COORDINATES: protein motif:HMM:NF025675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HBG8513277.1"
                     /translation="MFGSKTLEIIDGTIVVKGDSISETVSLESIKDVKVYEDMILIYI
                     SGFVAHIIPRRYLDKEIEKELMKELKKCKALEQLK"
     gene            6047..6358
                     /locus_tag="KRH39_003401"
     CDS             6047..6358
                     /locus_tag="KRH39_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8513278.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            6406..6723
                     /locus_tag="KRH39_003402"
     CDS             6406..6723
                     /locus_tag="KRH39_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG8513279.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
BASE COUNT         2683 a          690 c         1232 g         2257 t
ORIGIN      
        1 taatcacata cttaatgaag gatgagttac caaaattaaa aggtaactca tcctttttac
       61 atttgttagg aatgaattct ataaatataa aaataataat ttagatacag aaataaatat
      121 ttgaataatg acataaaatt acaaaaaata acaatgagaa tttgtataat actagatgaa
      181 tattaaaatt ttggagggta tttagagaat ggaagatagt caatatagca aagagtactc
      241 agaggactca ttatttaaaa aaataaaatc agtcttctct aaagcaggtg tatcggtaat
      301 atatggtgta ttattattat tttattcact taaagatcca tcagtaccaa tgggagcaaa
      361 agctactata ataagtgcat taggatattt tatatcacca atagatttaa tagctgaccc
      421 aattccagtt gcaggatatg gagatgattt aactgctata ataacggcat taggtgtaat
      481 ttctgtattt ataacacctg agactaaaag gaaagctagg gaaaaaacgt tagaatggtt
      541 cagcgatgca acagaagatg attttaatac tattgatgga caaatagaaa aaagaaatag
      601 tcaaaattaa aggcgatatt acataaaata ttagaatatt cttgttataa gaaaaaatta
      661 tagattaagg atactgaaat tatttagata agtaaatcaa tatatattta taaaggggaa
      721 tttaagatga aaatagcgag cattttaggc attattttaa taggagcatt aattttatta
      781 tctctaatag ggggatttgg tttaattaca tggataggta tgatgatagt tggaacaata
      841 gggattataa taggtggtat ttttaatatt ataggtatat ttatagcatt tggatttaga
      901 ttattattac ctattatagg aatagtatta gtaatattta taataagtaa aataattgct
      961 gttaaaaaat aagatatgga ataaaaatta aaagtaaata aaattaacta attcttgatg
     1021 tagataattt agaacgctcc gtagggtaag gtacggagaa ccgtatcaca agtaagtgcg
     1081 aactttacca aaattggata tataaatgaa aggtaggtct gctttaattc tgcaatgaaa
     1141 ctcacaaaaa tctaaaattt gaatttttgt aggttattgt agttttattc ataggttctt
     1201 cgtttttcac ctatttcagc aaattagaat ttgttttttc taatctatag tattgaaaga
     1261 aattgtaaaa cattccacaa atatattttt tatattcatt tcagatttag gttaaagtta
     1321 gtagttattc ttttgtgtat ataagttatt tgaagtaata aagtttcaat tgaatttgga
     1381 taaatatgaa tataaaagta ttgaaaaaaa taaaagttta tatataatgt aattatacta
     1441 aaatataaac atgaaaaata taaatgtaaa ggagatgtaa atatgcgcaa agaggtagtt
     1501 tttaactaaa ctaactgaat aaggattatt ttaaaaagta tatgaaaaaa atcttggata
     1561 actcaaggtt tatttgtggt agaaacaatt attaataata ttgatattac catgtctatt
     1621 tgtgtactct aaaaataatc agtattataa tgagcatatt acatctttat ttgaagaata
     1681 ggaaaactgt gttctaaaca cggtattttt atgcctattt ttactatatt tatagatatg
     1741 atataagact ttaatttgca tagcttaatc ataagttata taaattaaag ttgtatattt
     1801 catgtttata tttaggttat ataattttta tttaaggaga ttattttgat gatttatata
     1861 tttaaatatg aagattatga aaaacaaata aattttttta aaaatactag gatggtgatt
     1921 attaatggtt aataaacttt cagcatataa aacttattta ttattttcag ctattacagc
     1981 aatgtgtttt tcactagtag ctacagttat gatagtgtat cacattgaaa cggtgcattt
     2041 aaatccactt cagcttatac ttgttggaac tactttagaa gtagcatgct ttatatttga
     2101 aattcctaca ggaatagttg cagatgtgta cagtcgtaaa ctatctattg ttattggtgt
     2161 agttttaaca ggagtgggat ttattttaga aggttctatt tctagtttcg ttttcgtact
     2221 tgtagcacag attgtatggg gattaggttc tacttttatt agtggatctg ttgaagcttg
     2281 gattgcggaa gaagagaaag ataaagattt ggataaaatt tatataaagg gagcacaagc
     2341 aggacagata ggatcggtta ttggaatagt actaagcact gtaatagcta atttatctgt
     2401 aagactacct attatagtta gtggagtctt atttataatt cttgcattat ttttatggtt
     2461 atatatgcca gaaaataatt ttaaatcatc tgctccagag gatttaaata cttttaaaaa
     2521 gatgggatat acctttaaat ctggtcttaa atttataaaa aataaaccta taattataat
     2581 tttgctttca gtaactttat tttatggatt atccagtgaa ggttatgata gactttctaa
     2641 tatgcatttt ttacaagata ctatgcttcc taaacttgga aaccttaaac cagtgacttg
     2701 gttcggaatt tttggaattg caggaatgat attgagtgct atagtaatgc attttatgga
     2761 aaagaagctt aaggatgatg ataagaataa aaatggaaag ctgttattat gtataaatat
     2821 attttatata tcatttatgt tcatatttgc tattacaaaa agatttaact taatgctaat
     2881 agcttattta gcgacaagta cctttagaac tataaatgaa cctatattta gtgcatggct
     2941 taatggacat atagatgaca aggccagatc tactgtactt tctataaatg gacaaataaa
     3001 ttccttaggt caaattttag gtggaccaat tataggaatc atagctacaa atatttcagt
     3061 gagtatgggt atagcatgta cttcgttatt agtaacaccg gtattagtgt tatatattgt
     3121 tgctatgata atggataaaa aggtggttga tagagttgga ggtattgatt atgaagaaaa
     3181 taattaatat aggaatcgta gcacacgtgg atgcaggaaa aacaactata acagaaaact
     3241 tattatatta tagtggagct ataaaatcag ttggaagagt tgatttaggc aatacacaga
     3301 cggattctat ggagcttgag cgtaagagag ggattaccat taaatcatca accatatctt
     3361 ttaattggaa taatgtcaag gttaatattg ttgatactcc aggacatgtg gattttattt
     3421 cggaagttga acgttcatta agtgttttag atggagcgat actagttata tcaggagtag
     3481 agggtattca gtcacaaaca agaatattat ttgagacatt aaaggagtta aacattccaa
     3541 caataatttt tgtaaataag ctagatagaa ttggggcaaa tttcaataaa gtatttgaag
     3601 atataaagaa gaatatgtcc aataaagtag ttagattaca agaagtatat gatgtaggaa
     3661 gcaaagctgt ttatataaaa aaactatttg atacatgcat gataaatgat gatgctattg
     3721 atgttttatc agacttagac gaagcatttt tagaaagata tattggtgga atagaacctg
     3781 ataaagaaga aatacaagaa aagctttcat tatatgcaag tgaaggaagt ctatatccag
     3841 tattttgtgg tgcggcagca attggacttg gagttgaaga cttattagat ggaatttgta
     3901 gttattttcc atttgcaggt gatgattgtg aaagtgattt atctggagta gtatttaaaa
     3961 tcgaaagaac aagtaaaaat gaaaagaagg tttatgtaag attatttgga ggaaaaatat
     4021 ctgtaagaga taaaattcaa gtacctaata aggagatagc agaaaaagta aagaaaatta
     4081 ataggttaga aaatggggga gttgttgaag cacagaggat agaagcaggg gatataggta
     4141 ttttatatgg acttacaagt ttccaagtgg gagatgttat tggaatttca aatgataaaa
     4201 ttaaaaatat atctatagct aaaccagcat taaaaacaac aatttctgca attgataaag
     4261 aaaaaaatcc agagctattt aaagcattaa cattacttgc agaggaagat ccactactag
     4321 aattagagat gaatgacata gataaagaaa tttatgtcaa cttattcggt gaagttcaaa
     4381 tggaaatact aagttccatg ttagatgatt tatatggaat aaaagtagag ttttcgaata
     4441 ttgagactat ctataaggaa acacctaaag gttttggagc gtcaataatg catatgcagg
     4501 aagacttaaa tccattttgg gcgacagtag gcttagaaat agaaccagca gggagaggcg
     4561 aaggtcttag gtatatttct aatgtttcag tagggtcatt gccaaaatct tttcaaaatg
     4621 caattgaaga agcagttatt aagacaagta aacaaggatt atttggatgg gaggttacag
     4681 atgtaaaagt cactcttagc tgtggtgaat tttttagtcc agccagcact ccagcagatt
     4741 ttagaaatgt gacacctatg gtattcatgg aagcattata taaagcacaa actgttttat
     4801 tagagccatt acatgagttt gagttaaaga ttcctcaaaa tgctttaagc aaagcggtat
     4861 gggatttaga aactatgagg gcaacctttg ataatcctat tgttataggg gatgaattct
     4921 caataaaggg attaattcca gtagaaaatt caaaagaata taaaatgaaa atagcttcat
     4981 atacagaagg tagaggaatg tttgtgacaa aattttatgg gtataaggaa gcttcagctg
     5041 aattttcaaa agcacgcaaa aaaacaacgt atgatccatt gaataaaaaa gagtatttgc
     5101 ttcataaact aaacgcaatt agagattaaa tttaaataga taagttaaat ttttatatgt
     5161 aggatttaaa tataagacat tcaaagccta aaagtaaagg aagggtcgtc tgaagaagat
     5221 gaagaccctt ccttttttca tgttactatg cttaattgta agtgcttgaa atttgtaata
     5281 taatgtaaat aaagttttta ataataaggg gacgcacaaa tgaaaatcga ctttaaaata
     5341 acaaaagatg actatataag cttcaattta catcatttag aaaactcgaa atcacagaaa
     5401 agtacattta acatacttag atatgctgta cccatagtac tttctatacc aatctacttt
     5461 acaggaacgg ggatatttaa tcaaccgaat atatattgga ttatagtagc gatagttttt
     5521 ttagtcattt ggattttaac atatcctaag caatacaaaa aattagtagc taaagaaact
     5581 gataaactca taagttaagg agataattca tcaatatttg gaagtaagac tttagaaatt
     5641 attgatggga caatagtagt aaaaggggat tctatatcag aaacagtatc gttagaaagt
     5701 ataaaagatg taaaagttta cgaagatatg atccttatat atataagtgg atttgtagct
     5761 catataattc caaggagata tttagataaa gaaatagaga aagaacttat gaaagagctt
     5821 aaaaaatgta aggctttaga acaattaaag taaaacgtgc tcctttggag gtgtaacgga
     5881 gaaccgtatc ataaataaat gatacggttt ttgttagttt gcaacttaat aatattaaaa
     5941 taacaaaaaa gtattgaaag tatattgcat ttaaggtaga attaaataaa atattataac
     6001 attataatgt ttatatagtt gttaataaaa atttggagga aaatatatga taaaaataat
     6061 gttggcctgt tcagcaggaa tgtctacaag tttattagtt actaaaatgg aaagtgcagc
     6121 taaagaaaat ggtatagagt cgcaaatttg ggcaatacca gaatcaacta tacaaaatga
     6181 aatagaaaaa tgtgatgtat tattactagg accacaagtt agatatgtat tacctaaggc
     6241 tcaagaaatt gctaagccat ataatatacc agtagaagtt ataaatatga tgcactatgg
     6301 aacagttaat ggagaagctg ttttaaatag agcgatagaa cttaacaaca ctaagtaata
     6361 taaattataa tgtattttta atatataaat tgggagaatc aaaggatgga tgaaaagatt
     6421 atagaaatat cttttaatat aataggttat gcaggtgaag gtaaagggtt agcttttgaa
     6481 gcaataaaag aggctaagaa tggaaatata gaaaaagcaa aagaattatt aaaagagtct
     6541 agggaagtta taaataaagc acatagatat caaacagaat taatacaaaa cgaagctagt
     6601 ggaaacagga ctgaaataag tgtcatactt atacatgcac aggaccactt aatgaatggc
     6661 atgaattttc aacagttagc tgaggaaatc gttgatttac atttgaaatt acaagataaa
     6721 taaaaattaa agataataaa ttaagagagt tcttaaaaga tacattatgt gtagcacact
     6781 aattgttaga ttattgattc aatatttagt gagtaataca ttaggattat tttgaggact
     6841 ctttttatat ttagtttaaa gt
//
LOCUS       DAEORZ010000179         6772 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEORZ010000179 DAEORZ010000000
VERSION     DAEORZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.183"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..564
                     /locus_tag="KRH39_003403"
     CDS             <1..564
                     /locus_tag="KRH39_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HBG8513280.1"
                     /translation="LVKILINIICIITLTFSSLSIYIKLSEYKKADEVYTELRENTIN
                     NSKHQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFMDYR
                     NNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFSVYT
                     ADLINRDYLSIHFNSNYEFKDYLNYIT"
     gene            complement(1577..2239)
                     /locus_tag="KRH39_003404"
     CDS             complement(1577..2239)
                     /locus_tag="KRH39_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513281.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(2256..4811)
                     /locus_tag="KRH39_003405"
     CDS             complement(2256..4811)
                     /locus_tag="KRH39_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG8513282.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLFRLMGAKKRNVRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGMISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGRLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLIAVVVSNRDFESMNIIEGIKENE"
     gene            complement(5045..5239)
                     /locus_tag="KRH39_003406"
     CDS             complement(5045..5239)
                     /locus_tag="KRH39_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG8513283.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTKEN"
     gene            complement(5251..5430)
                     /locus_tag="KRH39_003407"
     CDS             complement(5251..5430)
                     /locus_tag="KRH39_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513284.1"
                     /translation="MIHIEISEKEFFRKIEEVFDKGEVNVEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(5435..5692)
                     /locus_tag="KRH39_003408"
     CDS             complement(5435..5692)
                     /locus_tag="KRH39_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513285.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSITKEKKDTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(5665..5805)
                     /locus_tag="KRH39_003409"
     CDS             complement(5665..5805)
                     /locus_tag="KRH39_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513286.1"
                     /translation="MVIAISLTLGLITGCTNKVSDTNEKIYTIEQISSIKKTCQRKYQ
                     NQ"
     gene            complement(5952..6772)
                     /locus_tag="KRH39_003410"
                     /pseudo
     CDS             complement(5952..6772)
                     /locus_tag="KRH39_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
BASE COUNT         2283 a         1030 c          691 g         2768 t
ORIGIN      
        1 ctagtaaaaa tattaataaa tattatttgc attattacgc taactttctc tagcctttcg
       61 atttacataa aattatctga atataaaaaa gctgatgaag tatatacgga actcagagaa
      121 aacactatca ataatagcaa acatcaagaa ctttatgata aaaataatga ttatagattc
      181 tggcttaaaa taaacaacgt aaatatagac tatcctgtag tacaaggata taacaatgat
      241 ttctacctaa ctcatgattt ttataaaaat tatcttcctt taggtagtat tttcatggat
      301 tatagaaata attttgaaaa tgataagagt ttaatagtct atggtcatta tatgaaaaat
      361 aaaactatgt ttggtcagct agaaaactat actgatgaag tatttttcaa agaaaataac
      421 ttagtagaaa taaattataa agtacaaact tatacttatg aaattttttc agtatatact
      481 gctgacttaa ttaacagaga ttatttaagt atacacttta atagtaatta tgagtttaaa
      541 gattatttga attatataac ctaaaaatca gtacacaaat ctgatataaa ggtagatatt
      601 tcagataaaa ttataacttt gtatacttgt aactatgaat ttaaagatgc tcgtactata
      661 gttcatgcta aattaatttc aaaaaaagag actgataagt aggtttgtgt aaatgcagac
      721 ttacctacag tcttttttga acataaaatt ggtaagtatg gaataatatt tatattaaac
      781 ttttgtaggc aaagaaatga aaaatcaaag agaaataatt attacagatt tagattacca
      841 agctgaatta agaaagtgta aaactatgga ggatgtagtt ggtgaagata gtatataata
      901 gtttaactta taaattatct tggtgatatt aatgaagttt caaagtacat attatttatt
      961 gatataaata aaattaaatc tgttgctaat agatgtacct ttgtttggag acaaactatt
     1021 gaagaaatat aactaaaatg ttgaagtagc tacctgactt tattttgaaa tgagaacaag
     1081 attttggcat taagataatt tagaaagaat cactggtaca caaggatgtg aacttagaat
     1141 gaatagaagt attcaggcat aaggagcttt cacccaaata aaacatagta tgaactttaa
     1201 aagaattcta atcagaggca atcaaaatgt tctttcatag tttatacctg tagtgttaga
     1261 catagtattt ataagtttca caaataagat aatcattaat aaatgtagta tgtatattca
     1321 tcctctcaaa aatatttttt aatcttacac tttgtaaatt attctatttt aaaatggtat
     1381 ttttatacct ttatttctta tgaccgtttt caagctcaaa ttttgcataa atataaatta
     1441 atgaaaagca agtaaaaaaa gaactgtcgc acagcaattt aaaattactc attcgacagt
     1501 ccttatttcg tggatttaaa tagtggagaa gaatcacaag aataataccc acgattaaac
     1561 ctttatacaa tacattttat atgactttac catcactaat agttattata caatcagcca
     1621 tcttagctat ttcatcatta tgagttatca taaccaacgt ctgcttgtat tcatttacac
     1681 aatactttag taaattcatc acctcattag ttgttttact gtctaaattt ccagtaggtt
     1741 catctgcaaa aattattgat ggcttattgg caagtgatct tgctattgca actctttgtt
     1801 gttgaccacc tgataattca ttaggaaatt tattaatctg actttctaat cctaacttag
     1861 ttattaaatc attaatataa ttattatcta tctttccttt agcaattaaa ataggaagaa
     1921 ctatattatc ataaacattg ataacaggta ttagattata gttttggaat ataaacccaa
     1981 attctttact tctaagatta gatagctttt catcacttaa agtatataaa tctaaattat
     2041 ccataaatac attacctaaa gttggtttat caagccctgc catacagtga agtaatgtgc
     2101 tttttccaga accactttgt ccaattattg cactaaattt atggggttct atctctaaag
     2161 taattccatc aacagccttt atttcatttt ctccttttcc atatattttt ttaatatcta
     2221 taacttttaa catcttcata catttacctc ccaaattatt cattctcttt tataccttca
     2281 ataatattca tactttcaaa atctctatta cttacaacta ctgctattaa acatacaaat
     2341 acagatatag caaagaatag taaagattcc ttaattggga atgtaaaaga cataaaattc
     2401 tctattccat agtttatata cgcatcatta ttccaatttg taaatttaac tagatttata
     2461 aatgatggaa tcatagctat tataaagcta aaaactgcat atgctaatgc ttcatatata
     2521 agcatttttt tcatattttt aagactaaca cctaatgctc taagcgttga aatttctttt
     2581 tttctaatta atagatttgt tcttatagta cagaaaatat taattgatgc tattattaaa
     2641 actagtgcaa ttattgctat ttgtgattta atgtaacttt tatatatact ttctgacatt
     2701 tcatgataac ttaatctact attaaataga gaccctggta tacttcttga aattttctgt
     2761 atctcattat taacaatctc atcatatgga ttttctaaat ttatgcctaa ttgagtgtat
     2821 ttttgttctc ctgttatgtt tttagagtga atattacttg ttataatttc tagatctggc
     2881 ataaaatcgc catctcccat agcaatccat tcatccttta aaattacagc cactcttacc
     2941 ttcatctctt tataaacaat tgtattattt tcttcaacag gaatttttac agttattatg
     3001 tctcctatat ttaattcttt gaatataggc tttatagtat gatcatcaac tatatcataa
     3061 aaataattat atactgctac atttggatat ccatcagtat cctttttaat atcatctaat
     3121 ctaccttctt caacaaagtc atttaacttt tcatctatat aatcatcatc ataaagcttt
     3181 aaaactaatt tatcttccat tatctctttt ttttctactc cattataggt tgtaaattct
     3241 ttgtttatat ccttattatt aaaagtaaac attccatttg ttatttgaat tttatgtata
     3301 cttttaacac ctttaatttt ttctatattc tctacatcac tatctgtgta ttttgatatt
     3361 tgcgggtctg catttactcc aaattgcatt gttatattat atctattttg catatcttca
     3421 attttactgt tgacctcttc ttgcatactt gagaatgtag taatgaatat atatcctcct
     3481 aaagcaatag acaacataga tatagcagct ctaaccttat ttcttccgat atttttaagt
     3541 cccataaatc cataaaatcc aaatattttg cttactagtc tgtctattcc aagtctactt
     3601 ctgtgatttt taattttgtc tgtatttatt attgattcaa taggtgatat cttgccgatt
     3661 ttaaaagttg gtataataca agatataaaa acagaaatca ttccaaccaa tatagctttt
     3721 ataactattt cattatttat atataattta gggttaagac cagcttcact gtataaaact
     3781 tcgttataat tactaattcc tatatatgaa tatgtaaccc cagctactaa cccaagtagt
     3841 atacctatta ttgttataat taatgcttga tagattatca tacaccttac gtttcttttc
     3901 tttgcaccca tcagtctaaa cagtcccatt tcttttactg attgatttag tgtaatattg
     3961 aaaatattaa atatgaaaat tatagatact gtagttatta aaacatttct tttaaaaaca
     4021 ttcggatcat tcttatcatc ttctacataa cgaagttccc ttacaagagg tacatttggt
     4081 agaaagcttt tttgtcctaa cttatatttt ttcataagat aatcacattc accttcaata
     4141 tttctccatc cacttttaaa acttaatata ctattataac taatcgcatc atgaggtata
     4201 atgttatttg tttcatcatt gccataagtt actgcttcgt attgccaaac cacatcatag
     4261 tacccttttg gtttttccat aactcctact attttaaatt ctttatttat agaaaataat
     4321 tgattacttc cgttagtatc tttatagttc ttttttattg ttaaatttaa tgtagatcct
     4381 actccttcac ttaaattcat tgcttctagt gcttttttct ctaatactat ttcattgttg
     4441 tttttaggta gtctaccttc tattattttg ttgtatctac tttttagata ctcttcgttt
     4501 gatgaagtta atataaatga gtttcctttt ttatctgaaa tacgtcctag attttgtacc
     4561 gaattaccct ttgatacttt ttcatcagtt tctaattttt tagcatcatc aagtgtcaaa
     4621 tcactaaact ctacatggta tccactgtaa agttcatgta ttgctactac attgttttca
     4681 ttttgtgaat cttttataac actaggtcca aatatcaaca taacacctaa cataactcct
     4741 attattaaaa gtaatgttct aagtttattt ttctttaaat atgctagtga catttttaag
     4801 taaatcccca tataatcctc ctattttact ataaattatt tatccaactc atctgttaag
     4861 tagattggtg gatattttaa atcttttaat tcttcattat tttttgaagc ttactaccat
     4921 cttcctcatg ataaacttgc agtatcacct tatttacatg tagtgcataa taatcaccat
     4981 tataaaaata ttagtattta taacctggat agtttttgta aaatgattta taaagtatcc
     5041 catcctaatt ctcttttgta tacttttctt ttgtaatacg ctctatatca tcacctgaaa
     5101 ctccttgata tgtcatatat ttatctatat atttttcagc ttcattccac tttctaaaat
     5161 agttgttgta tataataaat cctattatgg ctataatgct tataattaaa gttaataaaa
     5221 tagctttgta ttttctcata gtgaatcttc ctacttcaat ttaaattctt taataagttt
     5281 taattctaaa gacttagttt tatcatcaaa tttcttaact tgaattttta tatctcccaa
     5341 attatcaacc tctacattta cttcaccttt atcaaatact tcttcaattt ttctaaaaaa
     5401 ttctttctca cttatttcta tatgaatcaa tgatctatat gttataaatg gccattatga
     5461 atttatcatc ttctgacagc ttttcattat aagaaaaatc tttaacacat tgaattgata
     5521 atatttcctt cgtatccttc ttttccttag ttattgaata atgtaaaact tggctaggtt
     5581 gttcttttgt atactccaag tttcccaaag acaaaacaaa acttccatct gatttatttt
     5641 cagctaccct aagaccacta ttttctattg attctgatat tttctttgac aagtcttctt
     5701 gatactagaa atctgttcaa tagtatatat tttttcattt gtatctgaaa ccttatttgt
     5761 gcatcctgtt attaaaccta gtgttaagct tatagctata accatagtta ttgtaatttt
     5821 tttatcttca tataatcctc cttatatatt gaaaactcgt tatttctcat aaaaaaaaga
     5881 cgctatcttt ttctattaaa agataacatc cctatatgtt tagtatcaat atatgttccc
     5941 caactggtag gttaatcgac ccaactatca aaattttgat ttaatggtat ataaatattc
     6001 aatataaatt tgtctttttt atcttcaaat gataaatctc ctctatattt ttttatagaa
     6061 gatttgatgc tttaaatccc tattccatgt acaagtttat cttttttatc tgttaataat
     6121 aaacttttat tctttctaat tgaatttatt ttactattct cacatttaat tacgaaatag
     6181 gactttgaga tagttccttt tatttgtata tatttatcaa tacttttatc atgtatctta
     6241 tcgcaagctt ctattgcatt atctaagtat ttgcaaatat attagataca tctatttgtt
     6301 ttataaaatt cactttggaa aaattgatat cacaaatgaa tttaattttt cttttttcac
     6361 ttacatcact ttttttattt attattatat ccaatataac attacctgta ttgtaaattg
     6421 atttaaattc tttaatttaa tcttttaaac tatttacata cccatcaatt ttcctttttt
     6481 atatttgaga ttttctacac aatatatatg attatttata tcatgataaa gcttcttaac
     6541 tttagcgtgt gattcttgaa tacttagata atgagaatac tgcatatcta gcttattttt
     6601 tattacttca ttttgcgatt ttaacttatt ttccttaatt gtttttataa acactataat
     6661 aatgactaag ctactagata gtattaacct aggtattaca atatacccaa ctatatatat
     6721 ttcctaatac ttaccaaatc gatacatttt tctatttata taaattatta at
//
LOCUS       DAEORZ010000180         6755 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEORZ010000180 DAEORZ010000000
VERSION     DAEORZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.184"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            182..640
                     /locus_tag="KRH39_003411"
     CDS             182..640
                     /locus_tag="KRH39_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG8513287.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(757..1494)
                     /locus_tag="KRH39_003412"
     CDS             complement(757..1494)
                     /locus_tag="KRH39_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG8513288.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            1711..3387
                     /locus_tag="KRH39_003413"
     CDS             1711..3387
                     /locus_tag="KRH39_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBG8513289.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNIKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            3595..3795
                     /locus_tag="KRH39_003414"
     CDS             3595..3795
                     /locus_tag="KRH39_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBG8513290.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            3849..5012
                     /locus_tag="KRH39_003415"
     CDS             3849..5012
                     /locus_tag="KRH39_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG8513291.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(5208..5630)
                     /locus_tag="KRH39_003416"
     CDS             complement(5208..5630)
                     /locus_tag="KRH39_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBG8513292.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(5651..6487)
                     /locus_tag="KRH39_003417"
     CDS             complement(5651..6487)
                     /locus_tag="KRH39_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8513293.1"
                     /translation="MKQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIASMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
BASE COUNT         2587 a          797 c         1086 g         2285 t
ORIGIN      
        1 ttgtttttta ttaaaaatac caaaaaattt atgaattttg gtgaataata tatcttttat
       61 gggtatatat ttatattatg agaataaatt aaatttattt aaatgtcttg acaaaaaatg
      121 aattatcaat tacaatatta tttaataaag attatcaatt gaaaatagta agggtgatgt
      181 tatggcaaat acaatggatt tattaaaaga caagttaaaa gaaactggtt ttaagattac
      241 gccacaaaga agagcaatag ttgaaatatt gcttaaacat gaccattcac atctaagtag
      301 tgaggaaatc tatgatttag ttagggttga ctgtcctgaa ataggcttag ctactgtata
      361 tagaacaatg cagctattgg atgaaatagg attaatatca aaactaaact tagacgatgg
      421 atgtataaga tatgagatta gtctacataa agaagactgt cataatcatc atcatcttat
      481 ttgtaaaaat tgtggaaaga taatggaagc taaagaagac cttttagaca atatagaaaa
      541 agagatacaa tcattgtaca aatttaaaat acttgatcat gatgttaaat tttatggatt
      601 atgtgacgag tgtaatggag taagcgattc agaagaataa acttctattg cttaatgtcg
      661 atggaagaat agtatagaac ttttatattc tatgctattc ttccattttt tattataaac
      721 catctataaa atgcaaatat acagttttca aagtgattat agttttaata ttgaaaatat
      781 aattaatcct ataagtacaa aaatgaatga tttttctatg aaattactaa atggtcctcc
      841 aacagttaaa aaccctaaag aaattttctt ttcgcttagc ggataaaaaa ttggtattcc
      901 ttgctttgta aataaatctc ctaaaaacat atggcttgca taaccaatcg taccataaaa
      961 agataaatta ttaatattag tgattaattc tatctgtttt agtaaaaaat atactattat
     1021 agttgcaaat atgctatgag aaaaacttct gtgctttaac cagggaaaaa ttgacaacat
     1081 tagacaaaaa attgtaaaat atatttcaac ttctatgatg tataagcata tagctaatag
     1141 tataaatatt aatgatagaa aaacttttct tagaaatgtg tgggttagtt ttgcttcaat
     1201 aattataatt gcaacaaatg taattattgc gcttaaataa aaattatcat ttatatttat
     1261 tgaaataaag aaaataataa tattaacagc atatataaaa attctatata tgtatttgga
     1321 tacattgttt tttagaatga aatttgagac catagaatta gatttatcca aatcgggtag
     1381 tacagcaaaa gtagctgaaa ctaaaatatc aactaaagat ataggaattt taaatattaa
     1441 agatgtctgg atagctgtta agattcctat tgcacaatga gttttaccac gcataaatcc
     1501 tcctaaatta tccgatatta aattatataa tatttagagg atatcaaaat aaaataggtt
     1561 tgtcaaaata atacgttaaa tgtattttaa atatagctta aaatatgata cgaattatgg
     1621 tacaataaga ataactggta gcgagtgcgt gatttgatat tatttatgat tgtagaacgt
     1681 gttaaaaaat taaatagaca gaaggagatg atgcaattgt tcaaaaagaa caccaataag
     1741 ataaaggtaa tggccttggg tggacttaac gaagttggaa aaaatatgac tgttgttgag
     1801 tataaagatg aaataattgt tatagatgct ggacttagtt ttccagagga tgaaatgtta
     1861 ggagtagata tagttatacc agatataact tatttagtaa aaaataggga taaaataaag
     1921 ggtatattta ttacacatgg acatgaagac catataggag ctcttccata tatattaaag
     1981 aaaataaatg tacctgtata tggggcaaga cttagtatag gtcttataca agtaaaactt
     2041 aaagaacata agatgaataa tattaagtta aatgttatag gtcctagaca agttataaag
     2101 cttgataata tggaagtaga gtttttgaaa aataaccata gtataccaga tgcttactct
     2161 attgcaatac acacagacca aggtataata taccacacag gagattttaa gattgacttg
     2221 actcctattg atggagatgt tatggatatg catagaatct gtgagttgag caaaaaagga
     2281 gttcttttaa tgctggctga tagtactaat gcagaaaaac caggatttac tatgtctgaa
     2341 aagactgttg gggtaggtct tgacgaatta tttgcaaaag gaaatggcag aagaattata
     2401 gttgctacct ttgcatccaa catacacaga ttacaacaaa taataaatac tgctgagaag
     2461 tttaatagaa aagttgctat atcaggtcgc tcaatggtca atgtagttgg agttgcaaaa
     2521 gaattaggat atctagatat ttctgatgac atgcttatag atttaaatga tatatgtaag
     2581 tatgaagata gtgaattagt gataataaca actggttcac aaggtgaacc tatgtcagct
     2641 cttgctagaa tggctttttc agagcacaaa aaagtagaaa taaagagtgg cgatttagtt
     2701 ataatatctg ctcatccaat accaggaaat gaaaaattaa tatcaagagt aatcaatttc
     2761 ttatttgaaa aaggtgcaga agtagtatac agtgatatag ctgatataca cgtttctgga
     2821 catgcctgtc aagaagaatt aaaacttata catgcattag ttagaccaaa gttttttatg
     2881 ccagctcatg gtgaatatag aatgttaaaa agacatgctg aaatagctga acagcttggt
     2941 atggataaag aaaatatttt tgtcatgcaa acaggagatg ttttagaatt agataaaaac
     3001 tctgctaagg tagcaaatcg tatacaaaca ggaaacatat tagttgatgg tctaggagta
     3061 ggcgatgtag gtaatattgt tttaagagat agaaagcatt tatcagaaga tggtcttatg
     3121 atagttgtag ttactatttc taaggatgag ggtaaagttt tagcaggtcc agatataatt
     3181 tccagaggat tcgtatatgt aagagaatca gaagatttaa tggatggagc aaaggatata
     3241 attaagaatg ttctaaatga atgtgaagag aaaaatatta aagaatgggc ttatttaaag
     3301 aataatatta aagaaaacct aaaagaatat ttatatcaaa aaactaaacg aaatccaatg
     3361 atacttccta ttataatgga agtataatat aaaaaaacta taaatttgaa aattagacct
     3421 cttgtaattt ttaaagtaat atcaaaaatc caattgatat tatcttaaga attacaagag
     3481 gtctttttaa tacttgattt aaaaaaatat atatattaca taaaatacca attataacaa
     3541 tcttttatta gtaaaaacta cttatatata aattttactg ataggaggat tcttatggat
     3601 gatgtttcaa gacaaaatgc tataaaagct ttaaaacaaa caaaaatgga aatagcagga
     3661 gagtatggaa tgaattatga agatgctttt gaaataatag aaaatgcgtc taataaaggt
     3721 gttttagaag gttactttaa aaagcttgaa aagaaaaaaa atttaggtca aggtatatct
     3781 aggcatttag aataaaaaaa gttaaatgtg agaaaataag tttaaataaa ctttaagagg
     3841 tgacatcgat gaaaaaatta gctagtactg ctttggctat attaattgcg cttactccct
     3901 taagcttttc ttttgcaaac aataaggaaa atgctgatgc caaccaacta aatatatctt
     3961 caaaatcagc aattttgatg gatgttggta gtggacaaat tttatatgaa aaggatgctc
     4021 ataaaaagct tcctcctgct agtgtaacta aggtaatgac tatgctactt atagtagaag
     4081 ctttagattc aggcaagatt aaacttgatg atgaagttca agtaagtgaa actgcatcta
     4141 gtatgggtgg tagtcaaata ttcctagagc caggagaaac tcaaaaggtt gatacattgt
     4201 taaaaggtat agctgttgct tctgcaaatg atgcttgtgt tgctatggct gaacacttag
     4261 ctggaagtgt tgaagggttt gttgatagaa tgaatgctaa agcaaaagag ttaaatatga
     4321 atgatactca ttttgcaaat acaaatggac ttccagttgc aaaccactat acatctgctc
     4381 atgatatagc tttaatgtct agagaactat taaaacacga aatgataagt aaatacttaa
     4441 ctacatggat ggataaggtt gtagtaggga agaaacaagt tacagtaggt cttgcaaata
     4501 ctaacaaact tataaagcat tatcaaggag caactggtgt taaaactggc tttactcaag
     4561 aagcaaaata ctgtttatca gcatctgcaa agagaggtaa tactcatcta gttgctgcaa
     4621 ctttaggagc agagacttct ccagaaagat ttaacgacgc atctagcctt ttaaactatg
     4681 gatttgctaa ttatgaaagt gttaagttat gtagtaaagg tgataatata gcaacactta
     4741 cattggataa ggctgatgaa aacaaagtta aattggttgc taaagaagat ttaaatgctc
     4801 ttattaaaaa aggaagctct aaagaatttg agaagaaaat tgaaatagta aaaaatccta
     4861 agatgccaat taaaaaagga actgttttag gaaagataaa gatatgtaaa gataaaaaag
     4921 taataggcga agtggaatta ataaatacta aagatataaa taaggcaagc tatctacaaa
     4981 tgcttcaaag aataattgat aacatgatat aaataattat caaatgagat ataatacaca
     5041 gagatataat tataatacac ataaaaaata cccttatgta attatttgcc ataggggtat
     5101 ttttattatc atataaaagt atttcataat ttttatagac agatataaaa gtgtatgtaa
     5161 cttataatat catttggttt aatatcatgt acatgattat ttaatactta tcgaagtaaa
     5221 ggcatactac cagtaagttt attcactttc tttttattac cataaattgc tatcccaaaa
     5281 tactgtaact catcttctgg aacactagca atcttttgtg taaattcttc ataaagattg
     5341 caactttgtg ctacatctga aaaatctaca acagttaaat cgttaaattc caaagtatat
     5401 aattttttac gtaaatcttt tattatttct ttgtttcctt ttaaaatagg tacaggaatg
     5461 tcaataattc ctaagtgact attacctgtt ttgtcaatta cgtctggtcc aacagtttct
     5521 ggggcatgtt ttcctagtgt aattcccatt atagctgctg tattggatat aattcccatt
     5581 ggtaaatttt cgtctattac catgacacat tttgaatttt tagtttccat ttttatttct
     5641 cctttttacc ttatttaaag ttgtttgaat catcatttat aaatatattt tgatactgct
     5701 ttggtgtaag accaataaaa ttcttaaaaa agtttgtaaa gtgactttgg tctacaaatc
     5761 ctgtttgtat cgcagcatct ataggctcta cgcctttttc taaaagcttt ttagctttgt
     5821 ttacccgtat attttctagg tatctatatg gtgtaatacc ttttaatttc gtaaaattac
     5881 gaagtagaga gtatttattc atacttgcta tagtgcttaa ttcatcaagt actatatgtt
     5941 cagcataatt attttctaga taatcacata ctgcttgaat ttctatattt gtattttcca
     6001 agttagactg caaatttggc tctgtatgtt cttcaattaa ttgctcaatt aggaagaaaa
     6061 aaagctcttc ctttttaaaa tccaattctt cttccatgat tatatagtgt aattcttgta
     6121 gtagaggtac tagttcactg cgaaatataa caggttgatt aaattttggt aaataatttt
     6181 tacctgtaat ttcaaagacg gtttttttca ttatttctgg cttaatattg atacatctgt
     6241 aatcaagtac tttatcatct atttgttcac aagtatgact atcaaaaggg ttaaaaagca
     6301 gcaaatctcc agtacttgtg gtatactctt tatttttaca agataaatat ctttgacctt
     6361 tttctataaa accaattaca taatgttcat gaaaatggtt tgggaatttt tgcattatgc
     6421 ctttaaatct atatgcttca atttttaatt ccctatcaaa gcatactgtg cgttcctctt
     6481 gtttcactta ggtaacctcc tttcatgtta tacaaagtgt aacacatatt tttttatact
     6541 tcttgtatga tattgactaa aacataataa tgtcaattca tgtcaatata atattacatt
     6601 tacagtaggt gtaattatta tattttcaca atgaatatga tataataata aaagttataa
     6661 aataaaaagc aaattttaat tttatgccta gaagttaaaa ttattataat ataaaataat
     6721 tttgattata agttatttaa ttgttataat aatat
//
LOCUS       DAEORZ010000181         6710 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEORZ010000181 DAEORZ010000000
VERSION     DAEORZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.185"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..968)
                     /locus_tag="KRH39_003418"
     CDS             complement(<1..968)
                     /locus_tag="KRH39_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBG8513294.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSVAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINN"
     gene            complement(952..2463)
                     /locus_tag="KRH39_003419"
     CDS             complement(952..2463)
                     /locus_tag="KRH39_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG8513295.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENITKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMIENNVLKLAFGGKINEFNN"
     gene            complement(2517..3479)
                     /locus_tag="KRH39_003420"
     CDS             complement(2517..3479)
                     /locus_tag="KRH39_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG8513296.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(3527..4417)
                     /gene="rbsK"
                     /locus_tag="KRH39_003421"
     CDS             complement(3527..4417)
                     /gene="rbsK"
                     /locus_tag="KRH39_003421"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBG8513297.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAAKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(4459..5472)
                     /locus_tag="KRH39_003422"
     CDS             complement(4459..5472)
                     /locus_tag="KRH39_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG8513298.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(5701..6678)
                     /gene="bioB"
                     /locus_tag="KRH39_003423"
     CDS             complement(5701..6678)
                     /gene="bioB"
                     /locus_tag="KRH39_003423"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBG8513299.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEF"
BASE COUNT         1930 a         1310 c          677 g         2793 t
ORIGIN      
        1 atattattta tccatatagc aaataacatt accaaacctt ttataatcat ttgattatat
       61 gaagatacac ttaataagtt taatccattt cctaatattc ctattattcc gacacctata
      121 catgtaccta ctatagaacc agaaccaccc atcatattag taccaccaag cacaacggct
      181 gctatagcat ccaattctgc tccatctcca acagttggtg aacctgatgc caaacgtgat
      241 gatagtatta tcccagctaa tgctgttagt agtccagcta gtgcgtatac ttttactata
      301 actttattta cttttactcc acataatcta gcggcctctt catttccacc tactgcatgt
      361 acatgacgtc caaaattagt tctttttaat agaaaatgtc ctgctattac aactataatc
      421 ataattataa ctggtattgg tattggtcca atatatcctc taccgagcat cttaaatgca
      481 tctgtattta ctgatatagg tcttcctcct gtaaatacaa agcttgcccc acttattatc
      541 gttaacatgg ctaaagttac aatgaatgga ggtagttttt gtgttgaaat aattgcacct
      601 tttatgactc ctaatattgt ccctattgct aatgcaagta tcatacctac ccatataggt
      661 gcattataat tcactactgt tgtagcccaa aatgtaccta ctaaagcaat tactgctcct
      721 acacttaaat ctattcctcc tgtaattatt acaaatgtca tacctactgc aagtattgca
      781 tttattgata tttgtcttcc aacattaatt aaattatcta aagttaaaaa gttagatgaa
      841 gctacactca ttattataaa aattattaca agtcctacag ctacactccc ttggtctcta
      901 aatacacttg ttaattttgt caataatgga gaagattttt cttctttatg cttagttatt
      961 gaactcatta attttacctc cgaaagccaa ttttaataca ttattttcaa tcatttcttc
     1021 accttctaaa aatccactta tatgaccttc tctcataacc attattctgt cactcattga
     1081 tattaattct ggtatttcag aagatatcat tattatactt acaccactct ccactaaatc
     1141 tcccattaga ttgtatatct ctgccttagc atttacatct atacctctgg ttggttcatc
     1201 cataattaat atatctggtt gtaataaaag ccatcttgct attatagctt tttgttgatt
     1261 tccaccactt aaactttgta taacttgatt catatgtggt gtttttatct ttaaagcttc
     1321 tatgtatttt tctaaaatct ctttttcctt ttttctatct ataattgatt taaacttaga
     1381 tatctttttt aacccagcca tagttgtgtt ttctttgaca ctcatttgta aaaacaatcc
     1441 agtacctttt ctatcttctg ttattaatcc tagtccattt tctattgcat ctgatggagt
     1501 tttaaacttt acttcttcat tgtttaaata tattttccca gaatcatatg ttctaagtcc
     1561 aaatatagct tctactattt cacttctctt acttccaact agtccaccta ttcccaatat
     1621 ttcacctttt cttatattaa agctaatatc attaaaaaca ttgttcttag ttatattttc
     1681 cactcgcata atttcttctt ttatttctac ttttcttttt gggaaaaatt ggtctagctt
     1741 tctatctatc attaaattaa ctaattcttc ctcagacgat acatctttgg tattcaaagt
     1801 cgaaatcgtt ttcccatctc gcattatagt tattctgtca caaatctcaa agagttcttc
     1861 catcctatgg gatatatata ttatactaac tccactctct tttaaatcat ttataagtct
     1921 aaaaagtatt tcagtctctt ttttacttat tgaagaagta ggttcatcca ttattaatac
     1981 tttagtttct gatgataaaa ctcttgctat ttctaccatt tgtttttgag ctatactaag
     2041 ctctttgact aaactatttg gatttatatc tattcccata ttatcgaaaa tctttttagc
     2101 ctcttcattc atttttttgt cattggttaa gactttgcca tttatttctt ttcccataaa
     2161 aatattttgt gctactgtta attcgtcaaa aactgttagt tcttgatata tggcatttat
     2221 acctatcttt ttagagatat ctggggatat attctctatt tctttgcctt caaaaataaa
     2281 ttttccagaa gttttcgaat gtgcaccagt taaaatcttt atcattgttg atttaccagc
     2341 accattttca cccagtagtg catgaacttc gccatgaaat aactcaaaat taacattatc
     2401 taatgctctg acaccaggaa attctttagc aatattactt aacttcagaa ttatattact
     2461 cataatgaca ctccttttta aataggattt ctttcaaata atctcatttt attttactaa
     2521 ttcttaaagt cttttatatt ttccttatca taaagtaaag ctggtactgg tatattcttt
     2581 tctacctttt ccccttttat agcttttact actgcttcta cagctaaacg tcccatctct
     2641 ttaggttgtt gttgaatcat agcaagaata gttccatctg ttattccact tgatgcttct
     2701 tcagttaagt caaatcctac aatcttaaca tccttatttt ttgcagattc aagtgcagct
     2761 ttagctccta gtacactatt ttcagctgtt gcatatatta tatctacatc tggattagat
     2821 tgaattaagt tttctactgt attaagagat tcttctctgt catatcctgg ttgtgaattt
     2881 aatatcttaa catttgctga accttttaat gcatctttaa atccatctat tctctgcatt
     2941 tgtatttgag attttaaatc tgtcactatt gctatttctg actttccacc taacttttca
     3001 tctatgtatt ttttagtgta ttcccctaat tttttaccag catcataact aactgttcct
     3061 acataacttg taatattttc agtatttaat acagcatcta ctgtaactac tggcattttt
     3121 tcaaatgctc cttctactgc tggtactata gccgaattat cagttccaag tactatcatt
     3181 ccatctacat tctgttgtat aaaatcttct attgctgatg tttgcttagc tacatcttgg
     3241 tctgggtcat ttactataac tttcacacct aattctttag ctttttcttt tattccctca
     3301 tcaatatcta taaaaaattg atgttgaaga gttattgtag atactccaat agttaaatct
     3361 ttcttgtctg aaccaccttt attatctgat ttacttggtc cttccattga acaccctgtt
     3421 aacattccta ttgctagtat tccagcaatt cctaaagata aaatttttct cactctcata
     3481 attaacctcc atttttaata gttttaaatt tgaacaaatt agatttttat ttattattta
     3541 aatatttttc aacatcttct ttatagggca taccagattg tgcacctagt ttagttacag
     3601 aaagacctga tactactact gcatattcta ctgctgtata taaatttttc ccttctgtta
     3661 tagccactgc caatgctcca ttaaatgtat ctcctgctcc tgtggtatct tgaacatcaa
     3721 cacttataca agatacatgc ttgatttcct caccatcata aaatctagct ccattttttc
     3781 cttctgttat tacaagttta tttggatatt tttttaatac ttcttctatc ccttcatttg
     3841 tgtcaaaaac tatcttatat tcatgttcat ttggtgttaa gtaagttact ttttctatta
     3901 tatcttcatt tagttttgct gctggtgctg gatttaataa aaccctaatc ctattttcaa
     3961 aacagaaatt cactacataa tttattgttt ttaatggaat ctctaattgt agcaatacta
     4021 tatctgcatt ctttatctct tcttcattct ttttaattaa ttctatatct actagctcat
     4081 tagtccctgg tacaacaaca atactattat cattttcaca taatgttata acagcaacac
     4141 cagaactttt gtgattattt ctggatacta agctataatc aactttattc gttctaaaat
     4201 ttcttattaa ctcctctcct aagccatctt cccctatgca tgatatcatt tttacatttg
     4261 ctcctagttt agaagctgag atagcttgat tagccccttt tcctccaggt gatgtaaaaa
     4321 aactattacc taatactgtt tctccttttt ctggtctttt atctacagaa cataccatgt
     4381 ccatgtttac acttcctatg acaactatgt ttcccattac attactcctt tacatatcta
     4441 tttatgtctc tcgtgctgtt attcactttc ttttgttgaa tctcttatga ccaattctgg
     4501 tttgaaaatc aattcatcaa acgattcttt tttatctcct cttaaacaat ttatcaatat
     4561 ttcactggct ttatatccta tatcataaga cggctgtttt actgtggtaa gagaagggct
     4621 tattagactt gatgtttgaa tatcatcaaa tcccacgaca cttatatcat ttggtatact
     4681 taatcctctt tctttaagac ctcttatgac acctattgca atcaaatcat ttgcacaaaa
     4741 tattgcatca aaatctatct tttctaaatt ttttataaat tcttgtcccc attcaatctt
     4801 atattttcct tcaaatacat aagcaggatt gtagcttaaa ttgcactcga ttagagcctt
     4861 tttatatcct tctaatctat ctactgctat ttcattttga agtggaccac ttaggtatat
     4921 tattttctta tttccaatat ctaataaatg ttttgtagct atataggccc cttcaaagtt
     4981 atttatttta acaattcctt ttaaggtttc tttgttcata cttatcttct tatcaatcaa
     5041 gacagttggt atttttatat ctttataatt tgcttctttc cagtttgtat ttgaagatgc
     5101 tgcaaatata attccatcta tcatcctctc tgatagtgtt cttattcctt catattcttt
     5161 tttagcatct tcatctgtat tacataaaat tatattgtag ccttcttcat tagctttatc
     5221 ttctacacct cttgatacat ctgtaaagaa tggatttcgt atatctggta taagaagccc
     5281 tatagtatta gtttttttag ttactaaact ttgtgctaat ttatttggta cataattttt
     5341 ttccttcatt attttcaaaa ttttttctct tgttgcctga cttatatttt catctttatt
     5401 attaatgact tttgacacag ttgttttaga aacaccagcc aattcgccaa tttcttttat
     5461 agttatactc aagaagttca cccccaaaat ttaggaaacc gttttactta accggttacc
     5521 tagatactat cacatctttt tttcatttgc aaccatttta tataaatttt gatatttttc
     5581 ccttttttca aaatattgca acttataatt caattttaat atttaaatta atctgcaaaa
     5641 aaaaattaat atacttttat aattatctaa aagtatatta attaatttaa gcagtattta
     5701 ttaaaattca aatccttgct ctataatcat ttttttatca ttttcaatcc cacttccaac
     5761 agttgtcaaa taatcaccaa caattgcaga atttactccc ccattaaatc caactttgtc
     5821 atatcccttt aatgatagcc ttccaccagc ataccttatg tacacattag gaattataaa
     5881 cctaaatact gctgttgttt ttagtatttc cattggttct aatacatcat aattttccat
     5941 tggtgtattc ttaataggat ttaaaatgtt tattggaaat gattttacat ttaattctcg
     6001 tatttcaaaa gccattttta gtctctgttc ttcactctca ttcattccaa ttataccgcc
     6061 tacacataca tctagcccag cttttttagc atcttttata gtttttatcc tatctttata
     6121 tgtgtgtgtt gtacatatat tatgaaaatt atctctacac gtctcaacat tgtggtgata
     6181 tgtagtcaca cctgctgact tcaacatttt agcttgctca taatctataa tacctaaaga
     6241 tgcacataat tttaaattag tattttccct caatccttca tagatattta aaatattatt
     6301 aaactccttt cctgacatac ctttaccact tgtaaccaat gagaatctat gcactccttt
     6361 actctccatc tctttagctc tatttaagat ttcattgtaa tctaataatg aatattcttc
     6421 tacaccagtt ttataataag aagactgagc acaaaattta cagtcttcac tacatttgcc
     6481 agatttagca ttcattatag tacataaatc tgcttttctt cccataaaat attctcttat
     6541 ttcatttgca gattttaata gaatatcaaa atcgttttta ttatttgtat caaggcttat
     6601 taaattaaga gcttcttcat aacttatttc tttttctctc aatactttat tttttaattt
     6661 tattatgtat ttttccatat atcctcctat ttgtcaactt aatattttta
//
LOCUS       DAEORZ010000182         6663 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEORZ010000182 DAEORZ010000000
VERSION     DAEORZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.186"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            6..82
                     /locus_tag="KRH39_003424"
     tRNA            6..82
                     /locus_tag="KRH39_003424"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:Arg,seq:acg)
     gene            95..170
                     /locus_tag="KRH39_003425"
     tRNA            95..170
                     /locus_tag="KRH39_003425"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:128..130,aa:Val,seq:tac)
     gene            246..659
                     /locus_tag="KRH39_003426"
     CDS             246..659
                     /locus_tag="KRH39_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513300.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            968..4399
                     /locus_tag="KRH39_003427"
     CDS             968..4399
                     /locus_tag="KRH39_003427"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBG8513301.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            4641..6569
                     /locus_tag="KRH39_003428"
     CDS             4641..6569
                     /locus_tag="KRH39_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG8513302.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         2808 a          710 c         1256 g         1889 t
ORIGIN      
        1 aaaatgcgct catagctcaa ctggatagag tgtctgacta cgaatcagaa ggttaggggt
       61 tcgagtccct ttgggcgcac catatgaata gcgagggatt atagctcagc tgggagagca
      121 cctgccttac aagcaggggg tcacaggttc gagccctgtt aatcccacca gtgcaaacct
      181 ctagtaaaac tactagaggt tttatttttg attgaaaaag tttttatatt taggaggtaa
      241 aaaatttgta tatagtaata ggaaatgaaa tagttgatag tgaagaactt aaaataatta
      301 tagataaaaa tagtgaattt aaagttgaaa aagatttaag taaaagcaca aaaagagaag
      361 atgtaatagc atatcagtta agtatagatt taaattacct agattctctt ataaatgagc
      421 aatgtaattt agccagtcta tctgatgaag agaaatttga tgaatatatg actttgtctg
      481 atgaacttgc actggattta gaagagttaa tgccaaaata tacaataata aatgctagag
      541 catataaatt agacgaggta gatggaattg taaaaataat actagctgta gcgtatgcag
      601 atttagggca cttaaaactt tctgatgtag taaagaggct atctagacaa gtagattagg
      661 atatgaatat gaactatatt aaaaaaatgt ataaaatctt acgtaaagat gaaaataatt
      721 atgtaaacat agaaaaaaac agtataaaat caagtcaatt gtagaaattt tcacataaat
      781 tcatctgaaa ttctataaaa aatgccttaa ttttaatgag gtaaatcgag tttacacagt
      841 ctaaaaatta cttatattta taaatttatc ttattaatta aggaactttc ttaaacatct
      901 tcaaaaataa agtattttag tctataatct ttaatattac acaaacaata aaggagaaga
      961 atagagtatg cttaaaaagt ttaataaaat actagtggct aatagaggcg aaatagctat
     1021 aagaatattt agagcctgtt cagaattggg gataaaaagt gttggtatat acagtaagga
     1081 agataagtat ggattgttta gaacaaaagc ggatgaatca tatttgatag gagaaggtaa
     1141 agggccaata gatgcatatt tagatatgga tggaataata gacctagcaa agagaaaaaa
     1201 agttgatgct atacatccag gatatggttt tctagcggag aatgcagaat ttgctcgtaa
     1261 atgtgaagag aatggaatta cttttatagg accatcatct aaggtaatga atatgatggg
     1321 ggataagatt aactctaaaa aaatcgcaaa agaagttaat gttcaaacta taccaggagt
     1381 agaaaaagca ataagaagta ctgaagaagc aaaggaagtt gcaaacaaaa taggatatcc
     1441 tgttatgata aaagcatcta atggcggtgg cggtagaggt atgagaattg tacatcgcga
     1501 agaagactta gagcttgaat atgaaactgc ttgtagtgaa tcaagaaaag cttttggaga
     1561 agatattata tttatagaaa aatatatagc tgatccaaaa catatagaag ttcaaatatt
     1621 aggtgataat tatggaaata tagttcactt atatgaaaga gattgttcag ttcaaagaag
     1681 acatcaaaaa ataatagaat atgcaccagc tttttcgtta gatgataaag taagaaaaga
     1741 gatatgtgaa gatgcagtta agttatcaaa acatgtgggt tattctaatg cgggaacatt
     1801 agaattttta gtagatgcaa atggtgggca ttattttata gagatgaaca caagagtaca
     1861 ggttgaacat actgtaacag agatggttac aggaatagat atagttcaaa gtcaaatact
     1921 aatagcccaa ggttacagct tagatagtga agaaattaat ataaaatcac aagatgatgt
     1981 ggaaataaga ggttactcta tccaatgtag aataactaca gaagacccta agaataaatt
     2041 catgcctgat acaggtaaaa ttcaagttta tagaactggt tcaggatttg gtataagact
     2101 agatggagga aatggattta caggggcaaa cataagtcct cattatgata gtttattggt
     2161 taagacaata tcttgggata gaacattcca aggagcaata aataaaacta taagatctat
     2221 aaaagaactt agagttagag gtgttaaaac taatgtagga ttcctagtta atgttttaaa
     2281 taatcctata ttctcaaatg gtaaatgtag tactaagttt atagatgaaa atccagattt
     2341 atttgaaata acagaaagta aagacagagg aacaaaatta cttcaattta taggagatgt
     2401 aattgtaaat gataatgctt gtaaagaaaa accattattt gatgcattac atgacccaag
     2461 aatggataag gatggttcaa aatcagaagg aagtaaaata cttttcgata agttaggtaa
     2521 atctgcatat atagaaaaga taaaaaatga taagaaatta ctattaacag atacaacaat
     2581 gagagatgca catcaatcat tattagcaac aagaataaga acatatgact tattaaaagc
     2641 tgctaagcca acagaaaaat atcaaaagga tttattctca cttgaaatgt ggggaggagc
     2701 tacttatgat gttgcatata gatttttaaa agaatctcct tggcgtagat tacaaaagct
     2761 aagagaagaa ataccaagca taatgttcca aatgttgctt agagcatcaa atggagttgg
     2821 atataaaaac tatccagata atgtaataga agaatttaca aaagaatctg ctagacaagg
     2881 tatagatgta tttagaatat ttgattcatt aaactgggtt gaaaatatga aaccatctat
     2941 taatacagct ttagaaactg gaaaaattgt tgaagcaact atgtgttata caggtgatat
     3001 actagataag actaaaacta aatacaatct agaatattat ataaaaatgg cacaagaatt
     3061 agaaagcttg ggagcagaca taatagcaat aaaagatatg tcaggtcttc ttaaaccata
     3121 ttcagcttac acattagtta aagagttaaa gaaaaatgta aaagcaccaa tacatttaca
     3181 cactcatgat acaagtggaa atggtgtagc aacttgttta atggcgtcag aagctggagt
     3241 agacataatt gatgctgcat tagaatctat ggcaggactt acaagtcagc catcacttaa
     3301 tgctatagta gaggcactta agaatacaga gagagataca ggaatagatt tatttggata
     3361 tgatgagtta ggaaaatatt ataaggattt aagaaaagtt tataataaat ttgaaagtga
     3421 tttaactaat tcttgtgcag aaatatataa ttttgaaata ccaggaggcc aatatacaaa
     3481 cttaaaacct caagctgata gtttaggatt agttaataga tttgatgaag ttaaagaaaa
     3541 atataaagaa gctaatgaag ttgtaggaga tataataaaa gtaactccat cttcaaaagt
     3601 tgtaggagac ttagctatct ttatgactaa aaataaatta gataaagaca atataataga
     3661 agaaggtaaa aatttatcat tcccggattc tgttgtggat tactgtaaag gtatgattgg
     3721 tcaaccagaa ggtggaatac caaaagattt acaagaggta gttctaaaag gtgaagaagc
     3781 tataacagta agaccaggtt cattattacc agcagaagat tttgatgaaa tagctaaata
     3841 tctaaatgaa aagtatgata taaatgctaa tataagaaat gttataagtt atgctttata
     3901 tccaaaagta tatgaagatt atatcaaaca tctacaacat tataatgata tatctaaact
     3961 tgaaagtgat gtattcttct atggattaaa taaaaatgaa gaatgtgaag tagaaataga
     4021 agaaggtaag gtattaacaa taagattagt tgaaattggt gaagttaagg aaaatggatt
     4081 tagaactata ggatttgagt taaatggaat ggttagagaa gtagaaataa aagataagaa
     4141 tttctctgga aaaattaata atgttgaaaa agctgatatg aatgaccctc ttcaaattgg
     4201 agcaagcata ccaggaaaag ttataaaaat aatggttaaa gaagaagatg aagtaaaagc
     4261 aaatcaacca ttaatcgtta tagaggctat gaagatggaa actataatag ttgctaagac
     4321 tgatggagta attaagtcaa tcaaagtaaa agaagatgat atggttgaag ataaacaatt
     4381 attaatgata atgaagtaat ttaattaaaa taaattaaaa agcactctga aatgtttaat
     4441 aaaaaatgtt ttagagtgtt tttatttatt ttaattaaat atttgttaaa aaaaagagga
     4501 caattacctc cagatgtaga aataaagcta gtaaggaaaa tgttatctaa aaattctaaa
     4561 tatagtaaat attttccata atatttctta ggaaataaca gcaaagataa aatacataaa
     4621 tttaaaaggt gggtgcttat atgaaggttt atgatagtaa aatgttgaga aatgtagcgg
     4681 tattaggaca tagcggatgt ggaaaaacaa atctaatcga aactatagct tacacagcaa
     4741 acactaataa gatacctaaa ttaacagaca aggtaaatat gacttatagc atgggattaa
     4801 tacctataga gtataatgat tataagttta acttattgga taccccagga tattttgact
     4861 ttagcggaga cgttgtgtca tctcttagag caagtgatgc agcaataata gtaatagatg
     4921 caacagcgcc tattcaagtt ggtacagaga aatcattaga actaactgag agtattccta
     4981 agataatgtt tataaataaa atagataatg aaaaggcaag atacaaagat gcaatagcaa
     5041 tgttaagaga aaaatacaat aataagatag ttcctatgat aagtcctata tacaaagata
     5101 aaaactttgt aaagttacat aatgtttttg aaaacataga tgatttagaa ggtgaattta
     5161 aggaacaagc tatgagtgtt aaagaagcat taatggaact tatagcagaa actgatgacc
     5221 aaatattaga taaatatttt aatggagaag aactaactac agaagagata caaaaaggta
     5281 ttattatagg tatacaaaga ggtgatatta tacctgtaat atgtggttca actataaata
     5341 atataggcac aaaggaaata ttagatacaa tatcaagtta tttagaacct atctttacag
     5401 aggaaagcaa accatttagg ggattagtat ttaaaactat ggttgaccca tttgttggaa
     5461 agatgtcata tataaaaatt actgagggtg tactttctaa ggataaagat gtttttaata
     5521 taaataaaaa tgtaaaagaa aaaatagcta atatatatac attgagaaat agtgaattag
     5581 tagaaataga aaaagctaaa gctggtgata tagtagttat aactaaagtt aattctctaa
     5641 aaacaggaga cactatatct gcagataaag atgcagaagc tttagaaaag atagattttc
     5701 ctaaaccaca aatatattat gcagtaactc ctaagaataa aggtgatgaa gaaaaagtag
     5761 catctgtatt aaataaacta gtagaagagg accctacatt acattggtat agaaatacag
     5821 agacaaaaca agcactttta ggaggtcaag gagaacttca tataaaaaca attaaaaata
     5881 aaatgaaaga taagtttggt gttgatgttg aattgaatga ccttaaagtt ccttatagag
     5941 aaactataaa aggaacagct gatgtacaag gaaaacataa aaagcaatca ggtggtcatg
     6001 gtcaatatgg agacgtaaaa ataagatttg aaagatgtga atctgatttt gaatttacag
     6061 aagaaatatt tggtggttct gtaccaaaac aatatatacc agcagttgaa aaaggtttaa
     6121 aagactctat gcaaaaaggt atattagcag gatatcctgt tacaaacata aaagctacgc
     6181 tttatgatgg ttcataccat gatgtagact cttctgaaat ggcctttaag atggcagcaa
     6241 gtgcagcctt taaaaaaggt atggaagaag cacacccaat actactagaa cctataatga
     6301 aacttaagat aactgttcca gaagaatata tgggagatgt catgggtgat ataaataaga
     6361 gaagaggaaa aatatttgga atggaaccag atgacaaagg gaaacaaata atatttgcag
     6421 aagctccaca agcagagaca tttaaatatg caatagattt aagagcaatg acacaaggta
     6481 gaggatactt tgaaatggaa ttagagagat atggagaggt tccatcacag tttgcagaaa
     6541 aaataattgg gttagctact gcaaagtaat gaacaaaata aatagaaaaa taattaatat
     6601 atattattaa aaatattttt agggttttaa ctaggtatag gtttatgtta ggtaaactta
     6661 tac
//
LOCUS       DAEORZ010000183         6602 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEORZ010000183 DAEORZ010000000
VERSION     DAEORZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.187"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            167..1909
                     /locus_tag="KRH39_003429"
     CDS             167..1909
                     /locus_tag="KRH39_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBG8513303.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWELSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(2073..2267)
                     /locus_tag="KRH39_003430"
     CDS             complement(2073..2267)
                     /locus_tag="KRH39_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513304.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2264..2845)
                     /locus_tag="KRH39_003431"
     CDS             complement(2264..2845)
                     /locus_tag="KRH39_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8513305.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            3013..3831
                     /locus_tag="KRH39_003432"
     CDS             3013..3831
                     /locus_tag="KRH39_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8513306.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQVFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4573..5394
                     /locus_tag="KRH39_003433"
     CDS             4573..5394
                     /locus_tag="KRH39_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBG8513307.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLEDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(5704..6483)
                     /locus_tag="KRH39_003434"
     CDS             complement(5704..6483)
                     /locus_tag="KRH39_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG8513308.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNLYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     TKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         2465 a          892 c          845 g         2400 t
ORIGIN      
        1 tatttatcat tttaccagta taaatttcat atactttttt gaagttaaat tttttatatt
       61 atttataatc ttatctatat ctttatgtta aattttctta acatgcattt tttatattta
      121 atatagtaaa ataattaata actatatcca tgattggagg atttttatgg ttaaatccaa
      181 gaagaaaata tttgtttttc taattttaat tgcagttttt attatatttg catcatacat
      241 aagaaattat actaatgata caaaaaaaga ttctgttaga ggaactattc cttttgaaaa
      301 aatggaatat aagcgtccta atatacagtc aatttgcgaa aacattaaca agtgtaacga
      361 caaactttta acagctaaaa cagctaagga acaattaaat ttatttaatg aagttgataa
      421 aatttatcag gatttttaca gtacacttac tatagcaaaa attagaaata acatagattc
      481 atcagacgaa ttttactcta aggaatataa gtatcttatg agtaaatcag tagatgttga
      541 catgtcatac aaagactttc aagatagatt tgtaacttct aaattctcta aagaattaga
      601 aaaagaactt ggaaaggata catttaatta tctaaagaac attggaaaac ttaattctaa
      661 agaagttgaa ggtttgctta aaaaagagca agacctagtt gtaaaatatg aagatttatt
      721 agctaaatct acagtatcaa tagatggtat agaggtagac tttgaagaag ccttgtcaag
      781 accaaatctt agccctgaag aatatgtgaa aatttattca gattacttaa aaaaatacaa
      841 tcctatattt ggtaacatat tcttagaact tatacagaca agaactgaaa tagcaagtaa
      901 gcaaggattt aagaattata tagattatgc ctatatgaac ctaaacaaag actattctca
      961 agaagaagct aaaaaattta gacaagatgt aaaagattac atagttccat tatatagaga
     1021 gatttcatct aaaccatctg attcttctat ttatattaaa gtatataaaa atcgctcatt
     1081 taggaagttt gataaagtac ttgaagatat atctccaaaa ctaaaagaga gcttcgacta
     1141 tatgaaaaaa tatgacttgt acgattattc ttctggaaaa aacaaatcac ctggaggata
     1201 tactacttat ataaataaat ataaagcacc tttcttgttt aatacatggg acaattcttt
     1261 tttaggtgtt actagttttg ctcatgagtt tggtcatttt tataattact acaacagtat
     1321 taacttaaat aataagatgc aaccaagtat agatgtatgt gaagttcatt caacatcttt
     1381 agaaattcta ttttataaat attttgatga tttttttggt aaacaatctg aagctataaa
     1441 aaaagaacac ttatcgattg ttttaaatac tattattgat gcttgtcttt atgatgaatt
     1501 tcaagaaatt atatacaaaa atccatatat gagtctaaat gatatcaata aattgttttt
     1561 tgatttagag gaagaatatg gagtttccaa taatatactt agagaaaaaa atgctccttt
     1621 ttggatactt gtatctcata acttccaagt tcctttttat tacttgagtt atggtcttgc
     1681 atctgatgta tcacttcaga tatgggaatt atcacaagaa gattacagaa aagctgttga
     1741 tgtatatatg gattttttaa atcaaaatac tgatgctgga tttaaagatg tggtagaaaa
     1801 ggtaaattta caattacctt tccaaaatgg aaatttagag gaaataagct ctactttata
     1861 tgattatttt ggtatagata atccacttga actaaaaaat gcttcttaat ttctaatact
     1921 ttatactcat tcttaagatt tagtattaga ttgaaataag tgatattttt tcacacacaa
     1981 ttaatgttta ataaaattat atggtaaagt atcagaaatt tttaagactc ttgtttttat
     2041 aatttctgat actttattta ttaataatat tattattttt ttatatatat aattaaaagg
     2101 attgtggaaa tcaataaaaa tatccctgta aaaatagcac tattcatatg gaacttaata
     2161 gaacttgcat ctgttacctt attattctta atccagaacc cacaaaccat agttgaaaga
     2221 aaaagtaaaa cagtgataag acttgaacca atagttataa ttttcatctt acatcaacat
     2281 cctctccaat aatattttct attaaaatat caatgaattt atctcgctca tatttattct
     2341 ttatatttat accactgtat aactgaaatg attctgtaga taaagcagaa atctgtattg
     2401 gtaaaataat ttgcagggca atgattctta gactcatttc atctttttta tttccaaaaa
     2461 ttttacgaag catagggaca atatcaagtt ctgcttgcat atctccattg gttatacatt
     2521 gattcaatac aaaaggaagc actttttcat aatgtaatcc agtatcacac aattttttta
     2581 ataaattttt taaatcttca ataggtctta gagtatatac actatcgtca tatagctctg
     2641 ctattatatt tgacatcaca tctcctatgg cagtactcaa caaattatcc tttgttttaa
     2701 aatgataatt tattaaacca actccaacat ttgcacgctc agcaattttt ctaactgtaa
     2761 tctcctcaat atcatctact tcatttaaaa gattcaatgt aacttcaatt atacgttctc
     2821 tagcatcttt atcattactt ttcataatag acctccaaaa caattaacat tttcggttct
     2881 gaacatgttc agtataatat ttttttcatc atatgtcaat ataatttaat tataatttta
     2941 gttgactcta acattatgat atggtttata ttaatattaa gctaaaagta cctttataaa
     3001 ggagacaaaa acatgtttaa gataggcgat ttttcaaagc taagtaaaat tagtattcgt
     3061 atgttacgtc attatgatga aataggactt ttaactcctt ctcatacgaa taaaactaat
     3121 ggatatagat actatagtgc agaccaactt tctactacaa acagaatcca tgcactaaaa
     3181 gatatgggct ttggcttgta ttctataaaa gaaattttga cagaatataa cgataaagaa
     3241 agtctcataa aatatctaaa tatccaccat tctcaagtaa aagaacaatt agaagataca
     3301 cagaaaaaac tattaaaaat agaaaccact attaaaagaa ttggaggaaa tgatattatg
     3361 aaaaattatg atgtaactat aaagaatttt gcaccaaaat atatgatgac acttagaagg
     3421 gttataccaa cttaccaaga cgaaggaatg ttatggcatc aagtattttt agagacaaaa
     3481 gaccagaatg ttcaaattga acccccaaag tattctaaag cagtttttta tgatactgga
     3541 tataaagaag attatgtaga tgttgaagta caagttgctg tatctggaaa gtataaagat
     3601 actgaacatg ttaaatttaa gactgttcca agtgtgactg ctgcaactgc aattgttaat
     3661 ggaaatttca atcaagttgc agacgcttgt gaggctattg gtaattggat atcagataat
     3721 aactatgatg tggatggtcc tatgtttaat atttatcatg taagccctgg caacgatagt
     3781 aatcctgata attgggtaac agaggtatgc ttcccagtta aaaagaagta atttttatat
     3841 taattggata atattttaat ttgttagaca aattactttt agtgttgtgt ataaatacct
     3901 acaatttaat tagtatatta tcttaatttt tcagataaat cacttgtgat gttgtgttta
     3961 aatgactaga atttgattag gatattatct taatttgtca aataaattac ttatgatgtt
     4021 atgtttaaat gactagaatt tgattaggat attatcttaa tttgtcagat aaatcaatta
     4081 taagattata tactatcctt atactctagt taaatatatt ttaatcacta atataaatta
     4141 gtttttttaa caaaacaata aaagagtaaa gtacaaataa ttcctttgtg ctctactctt
     4201 tttaactttc tttagacaat aatacaatga taaatgtaaa aaattatata ttccattcta
     4261 tagaaatatg ctcaccaata acatgtatgt tgttaatcaa tatattatat caaattatat
     4321 caaaattaca taaaccttat aggacaatca tatcttatta caagaaaaat gtcttatcca
     4381 tattgaataa gtcattcagt tcatatacaa agtcaatttc attgagaaac atttaatata
     4441 tgtttataaa aatttcatac tattctttca tatttgcatg tgatataatt tattaactca
     4501 taattttgtt aatcagttgt gcttgtatat tatttatata atctaatatt ttaagaaggt
     4561 ggtgtaataa ctttgaaatt aaaaattgtt ggtagtggcg gtatgtcaat tattccaagt
     4621 tcattttgca aatgcaaaat atgtgaagaa gccagacaaa aaggcggaag atatgaacga
     4681 ttagggccaa gtttatatat tgatgatata aaaatgctga tagacacacc tgaagacatt
     4741 gcagtagctt gtaacagaca aaaaatatcg gaagtaaaac atatttcaat ttctcaccat
     4801 gacccagacc atgttaaggg tattagaatt gtcgaaaaaa taggatgtga ctttatcaca
     4861 ggagaaaata atccaattgg cttttatgcg cttcctgaag taattgaaga tataaaccga
     4921 acgaatctcg attgtcttaa atattatggt gatgttataa attgcatttc tattaatgaa
     4981 acttcgcatg taaagataaa taattttgat attgatttga ttaacaataa cccagacagg
     5041 aatataacct tctatgttat caaagagaat gaaaaaaaag tcatatatgc ctgctgtaat
     5101 cccaagccat ttactcatag tgatatgtac tttgatgctg atgttttaat aataagtcta
     5161 gtttctgatg atggtatatt aaatgatggt acaaaacttg aagacacacc atttaaagat
     5221 gaaatattta ctttagatga gattgttgaa attaaaaact attatagaat taaaaagatt
     5281 attgttacac atattgacga aatgtgggga aaatcatatg gttattacag tgaattagaa
     5341 aagaaacttg ataacattta ttttgcatat gatggtatgg aaattatagt ctaaatcatt
     5401 aagtaataag cataatgcaa aataagaaaa cttcaattta acagtatagt taaactatgt
     5461 aatatcaaca tatctcagtt tttacaaaat atcatagtat ttatctttat aaaatttcaa
     5521 gtcctacata ttttaatact tcaatatcta aataaagtaa aatcaaaaat atattttata
     5581 tacattatag acggcttatc ggcactagat aagccgtttt gtcacaatta ttatttgctc
     5641 aacaaaactt ctcataatat ataacagatt gaatctccaa ctgatactta attaccgaaa
     5701 ctcttattcc ttaatatgtt gttgcattaa ccaaaccttc atcatcattt tctgtggcaa
     5761 tagttttgct aataaatgac tggattttgt atttatacca tacacagaaa tatccttatt
     5821 tttctttgta tccttcaatg cttttttggc aacaacgtct ggagacacta tgcctgggaa
     5881 attttttgtc cctttttcag ctacaactat acctctctta aatagatttg tactcatcca
     5941 acctggacat acagcaattg catttattcc tttactctta agttcaacat ttaatgctct
     6001 tgtataattt cttataaacg ctttagttga actataaaga ttttgatatg gcaatggctg
     6061 gaatgatgct tgtgaagaaa tattcatgat atggcttccc ttttgcatat atggaataca
     6121 tataagtccc aatgctacaa cagcactaat atttaaatca atcatattaa tagattcatc
     6181 tatacttaaa tcattgtatg aacagaattt tgcaaaacca gcattgttta taaggtattt
     6241 tatacataca ttttctttac ttaaaaatgt accaatttct tttacttctt ctatttttga
     6301 taaatctttt gaaaaaattt ttaccttact tccaaattca tctactaatt gatttaatct
     6361 cgccttgttt cttgcaactg cccatatttc tgttatttct tcttcattta ttaataactt
     6421 tagaaactct ttaccaaaac cactattagc acccgttata attgcaatct tcttataact
     6481 cataattatt ctcctttacc ataagtttat acgctcaaac tgtaaactta ttttttacaa
     6541 ataaaacacc ctctattcag atgttatatt catatcattt gagatattat acttatatca
     6601 tt
//
LOCUS       DAEORZ010000184         6589 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEORZ010000184 DAEORZ010000000
VERSION     DAEORZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.188"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            230..694
                     /locus_tag="KRH39_003435"
     CDS             230..694
                     /locus_tag="KRH39_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513309.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYKNNIQFYTTALDVLEDMKKKILRERKYKKRR"
     gene            complement(1023..1184)
                     /locus_tag="KRH39_003436"
     CDS             complement(1023..1184)
                     /locus_tag="KRH39_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513310.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(1236..1688)
                     /locus_tag="KRH39_003437"
     CDS             complement(1236..1688)
                     /locus_tag="KRH39_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513311.1"
                     /translation="MREKASEIEKLQLVYNSTSMLKELLDDAGFDNKSKLLTAKTLYK
                     KAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPAQMAVCEIIKKIDLEDGEVKGVW
                     ETNGSWTGTVNKYTKSVIDKVRTWIEENNRPTKIAGEKKNYHVFYKIE"
     gene            complement(1685..2050)
                     /locus_tag="KRH39_003438"
     CDS             complement(1685..2050)
                     /locus_tag="KRH39_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HBG8513312.1"
                     /translation="MKNELMMFEGKEIEVFEFEGRILFNSKDVANCLDIKNVNENIRL
                     MNEKQVVKLRNSDISNTDIRKLNNAGENFLTESGVYKLIFKSRKEEAERFQGWISDEV
                     LPSIRQTGAYINNNAIPKS"
     gene            complement(2171..2359)
                     /locus_tag="KRH39_003439"
     CDS             complement(2171..2359)
                     /locus_tag="KRH39_003439"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513313.1"
                     /translation="MNKIATKSRAEYMKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNL
                     TKKEWLEEKIDEELEQKE"
     repeat_region   2514..3862
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2579..2607
                     /rpt_unit_seq="atttacattccatatagttaatctaaaac"
     gene            complement(4129..5051)
                     /locus_tag="KRH39_003440"
                     /pseudo
     CDS             complement(4129..5051)
                     /locus_tag="KRH39_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
     gene            complement(5245..5397)
                     /locus_tag="KRH39_003441"
     CDS             complement(5245..5397)
                     /locus_tag="KRH39_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513314.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            complement(5671..5994)
                     /locus_tag="KRH39_003442"
     CDS             complement(5671..5994)
                     /locus_tag="KRH39_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513315.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            complement(6030..6284)
                     /locus_tag="KRH39_003443"
     CDS             complement(6030..6284)
                     /locus_tag="KRH39_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513316.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
BASE COUNT         2192 a         1120 c          615 g         2662 t
ORIGIN      
        1 aagattagat attacaatat tagcaataat attagtagcc attataacaa cagcattata
       61 ttatctaatt agaaagaaat atataactat ataaaaaata atatcttata aataataagc
      121 ctatcattat actctatttt tagctaaaat tgttcgacaa attgctgata taagttctta
      181 aatatgctat aaattaaagt atattatata acattgtagg agggatttta tagattttgg
      241 ggaagttaaa tcagagttaa gtgacaaaga ccttaaagat aatgaagaag ctttaaacaa
      301 gtataaaaaa tattatttaa aagtttataa gtattatagt gaaatggatt ccataaaaag
      361 tattcaagat atagaaatag agcaatctag gcttaaagga cagttacaaa aaaatagtaa
      421 ccccattttt gctttgtata tatctattat ggctgttgta ttttctacaa caattaactt
      481 aacatttgat ttatcagata tcactaatta ttttattaga atgattatga cacttttatt
      541 tattgtactt cttatttttt tagttaacaa gggcattctt ccaatagcta taccttataa
      601 aaataatata caattttata ctactgcatt agatgtatta gaagatatga aaaagaaaat
      661 acttagagag agaaagtata aaaaaagaag gtaacaacta actctagttg ctaccttcta
      721 atctatttaa ctaagttttc tacttcacta aatgctttct catttccatt tccgccaatt
      781 tgagttatat ttctaaattc tcttttattt attaaacttt tttgagtatc acttattcca
      841 tctccaacta atcgtactag agattctgtc ttacctgcaa gtactcctac tgataaagcg
      901 tcaactaaat catcttctct attttctcca tttttgggtt aatataattc tctatctcca
      961 ttataccatt ttataccaaa caaagcactt gaaacagtag aatatccaag tgctttatat
     1021 gtctatttat tatttttctc taattttttc tcagaaagtt tatatactat actttctaac
     1081 ttattatata tatctatatc tgtttctctt attttcaaaa gtttaattct atattcttct
     1141 tttctctttt caatatacat attatcaagt aaattctcac acattgtatt aatccccaca
     1201 aaactaaact aaattattta atacaactga taaatttact caattttgta aaatacatga
     1261 taattcttct tctcacctgc aatcttggta ggtctattat tttcctctat ccaagttctc
     1321 accttatcta ttacactctt tgtatattta tttacagtac cagtccaaga accattagtt
     1381 tcccaaacac ctttaacttc accatcttct aaatcaatct ttttaataat ctcacaaaca
     1441 gccatctgag ctggtttatt actcttagaa tatattttca gtttagatgc tatttgtttt
     1501 gtatcaaaat aatgttcttc ttcgtttatc tctattggta aatcaattcc tgctttctta
     1561 tataatgttt tagctgttaa tagttttgat ttattgtcaa agcctgcatc atctagcaat
     1621 tcttttaaca tagatgtact gttataaact aactgtaatt tttcaatctc acttgctttt
     1681 tctctcaact tttcggtatt gcattattat ttatgtatgc accagtttgt cttatgcttg
     1741 gtaaaacttc atcacttatc cagccttgga atctctcagc ttcttctttt cttgatttaa
     1801 agattaattt atacactcca ctttcagtaa gaaaattttc tccagcatta ttaagttttc
     1861 ggatgtcagt attactgata tccgaatttc ttaactttac aacttgcttt tcattcataa
     1921 gtcttatatt ctcattaaca ttttttatgt ctaaacagtt tgctacatct tttgagttga
     1981 ataaaattct tccttcaaat tcaaatactt caatctcttt tccttcaaac atcattaatt
     2041 catttttcat aatattacac tccttaataa ttgatttttt ttaaggaatg acatatatta
     2101 taattgatat acgatagtat acgtcatagg ggttactcaa actttggtcg gtgggagtga
     2161 cccttatttt ttattccttt tgttccagtt cctcgtcgat tttttcttct agccattctt
     2221 tcttggttag attcttttcc tctaatactt catcaaattt atctaacttt tctttgtcta
     2281 aaagtacact aaaacctctt ttatcttttc gacgattttt catatactct gccctacttt
     2341 tagttgctat tttattcacc tcttttctgt aactcgttac attaataaca atattgtaac
     2401 gagttacagt caataggttt ttactaactt tttctaatta ttttacccaa ccaaccaatt
     2461 ttgagcagaa taaaagcacc tacatatttg taagtgcttt ctttgtttat ttaattttga
     2521 atccacatag ttaatctaaa acacaattat taattgtttt attagtgaaa tcacagcaat
     2581 ttacattcca tatagttaat ctaaaactga ccaacaaata tagtattctc gccaatcatc
     2641 aagatttaca ttccatatag ttaatctaaa actgttaaca aggtaaaagc tatagtaaat
     2701 acaataacat ttacattcca tatagttaat ctaaaacact ttttcattag tatttaaatt
     2761 ttctattaac tcaatttaca ttccatatag ttaatctaaa acgcatatat gctctatatt
     2821 tttcatcagt ttcaagttta tttacattcc atatagttaa tctaaaacct ggcattctta
     2881 aatctctttc atattgagac aaggctattt acattccata tagttaatct aaaacgctat
     2941 ggagaatatc tagaatggtt gggagaatgc aaatttacat tccatatagt taatctaaaa
     3001 cgttttactt ctgtattagc taatactatc atgtcatcat ttacattcca tatagttaat
     3061 ctaaaaccat ctgataatcc tatggagggt ggttattatc aagctaattt acattccata
     3121 tagttaatct aaaactttaa tattccagac gaaacgacag ctactgaaag cgatttacat
     3181 tccatatagt taatctaaaa cctatatttg ccgtccgtac ctttcgttct atatcctaat
     3241 ttacattcca tatagttaat ctaaaactca tttcactttc tcccaaaaaa aacactcctt
     3301 tcttatttac attccatata gttaatctaa aacttatata tagataccat ctttgctact
     3361 atttgattgt tatttacatt ccatatagtt aatctaaaac gagcatacga aagtcaaaac
     3421 gtagataaat atcaattcta tttacattcc atatagttaa tctaaaacca acttcacagt
     3481 tgagagttgc aaaagaataa aataatttac attccatata gttaatctaa aacaagaagc
     3541 atttaattta attgcacaag gggcgcaaaa atttacattc catatagtta atctaaaact
     3601 ttcataacct cacaacactt atcactgatt tcaaaattta cattccatat agttaatcta
     3661 aaacactttt tcatgatagc agttggtgga gttgcatttt tatttacatt ccatatagtt
     3721 aatctaaaac aagtgtgagg tgaaattgta tgatttattt ttatagattt acattccata
     3781 tagttaatct aaaaccctcc atttatttta tatattaaaa ttatagcatt cgaatttaca
     3841 ttccatatag ttaatctaaa accccaaaat aaacttagca tttccaatac ctacatatat
     3901 acaatactct taaaattgca gtgagcgggc aatagtgcaa ttgataacac ttatcacgca
     3961 ccctcaacat cttagatttc aagtattaag ccctactttg taacaaatat cgcacactgc
     4021 aaaacttcta tatttttatt atatcataaa atctaaacta taaaattaaa atgtaataat
     4081 ttgtaaattt aaaagcacct acatacttgt aagtgcttac tatattatct attctaataa
     4141 ttcttttttc tttttattat attcttcctg tgtaattgct cctgaatcta gcaactcttt
     4201 tagtttcttt attctatcta gtgggtcaac cactctgttt tgaatgtagt tattataact
     4261 gttttttctt gttctatttt ctcttgttct cgcttctctt cttcttcggt tttaagttca
     4321 aactcattga attctaaatt ttcaacatta tctacattat tttgtatgtt tctaaaataa
     4381 tcaatataaa cagaattata tataccaccg tcatcttttg aatgaaaatt aaaataagcc
     4441 ttgcttattt taattttttt aagatcatta gcaatttctt ttacaaattt tttacattat
     4501 tcagaattac taaatttttc attttgtaca gttaaatcaa aatacatact atatctttta
     4561 aattttccta cttttaattc atatttcttc cccttatatt cttgaggtac taaaacctct
     4621 agcttaactt ttaaatcatt ttctgatatt tccattccat gtttttcaat atatctttct
     4681 ttgtctactt cttattgtat tcttcttctt tccatttctt tttctctttc tacagtttct
     4741 ctttgtgcct taacagtctc aactttatta tctgctatca aggatttatc ttcccaagtt
     4801 gcacaaagat aaattgaata agtgaaaaaa agaactgaca caagacccat aactattttc
     4861 ttttctataa aagaatatat aatcaacaaa aaagctatca taactaaaat atattgcata
     4921 gaagaaacag ctattataag taagactatt gttattattg gtactaatat ccacattgta
     4981 aaattccccc acaaattact catctcactc aatattatag cataatttgg caaaacaaaa
     5041 gcacctacca tttaagtaag tactttctta tttacaatta aatttattca tctagttaga
     5101 ttcatcatca ttgtgtataa aaaatttcat atttttattg actataaaaa atattatatg
     5161 caatattttt tctaatttgt ggtataataa aaacaagaag aactacaatc tatttgcggt
     5221 agagtgaagt tctataactg aaagttattt tttacgatga tatttaaaat ttatatcaat
     5281 ctttaagtca ctctttgcgg tagagtggct ttttactttt ttgattaact tgctaatcaa
     5341 ataaactatg taactagctg ttacactagc taatacatta aataaaaaat tatccatgta
     5401 tattcacctc ccttcttatc gttgggagga taatttttta atacatgaac tccactctat
     5461 aaattgtaga ttacatcttc ttgctacaat tattataata tataattctt acatattttt
     5521 cctatatctt actttttatt ttattttcta ttttttcaat aaaaagcacc tacatatttg
     5581 taagtacttt ctatgtttat ttagttttat tgattactta ataggttagt acaaattcca
     5641 taccaaccta agaatatcta cataaaataa ttaaataata taatcaataa gtattttttt
     5701 cactctttta atcaaattat tacctgttcc ccaatcaatc tgaaatagtc caattgcact
     5761 accagttgag gtaattttaa caagtgtttt tcctgtagta ttttcacaaa tcactaccaa
     5821 acctgcatca ctattgcttc ttgcataata tttttcaaag attgtaacta ttatagactt
     5881 attattttct aggttataaa tatcaacaga aactaaatct gcttccaaat ttttttctac
     5941 cattttctgt gcttctagtg gtgtcatctt tacactaaat acatctcttg ccatatcatt
     6001 catcacatcc taaataaaaa tatacttttt tagtattttc tgacactttt atcaaacagt
     6061 attcctattc ctcctagtaa aaccagtata gaaccaaaat ttctatattg tgaaatagat
     6121 tgttctaaat atatgttgta ttgttcggta gctattgacc caccattttg ttttataata
     6181 caatccatat taaattgacc tagacctata cagcaaaata acactattat tccaattaca
     6241 aaagcaaata tacatattgt aagtttaata tctttatttt ctataaatac acccctcata
     6301 atttagattt atttgttgtt ttttacttgg tgatatatta tctacattat actatacaac
     6361 atatatatcc atctatttcc aaaattgtaa cattttcata tattagcaat gtgactattt
     6421 taactttaaa ttctaccttc ttctacctct ctttttctct ctttcagctt ctttcattgc
     6481 ttcttcttca tcttcaattt taataagtat tgagtcggct gctaatgctc tctcattaac
     6541 ttctaaattc atatattcac taggtttcca ctttagcttt tgaatgcaa
//
LOCUS       DAEORZ010000185         6430 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEORZ010000185 DAEORZ010000000
VERSION     DAEORZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.189"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            6..761
                     /locus_tag="KRH39_003444"
     CDS             6..761
                     /locus_tag="KRH39_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG8513317.1"
                     /translation="MVVGVRVSSLNMKENEISRNAKLSSSTITDIVSNTSIEVSKGPS
                     KSSGKIAYITIDDGPSKFTDEMIKTLNKYNVKATFFMIDGNMKEYPQQVKNIIKNGNT
                     AGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFYKITGKHSKVIRIPYGSKPYTPQASYQ
                     ALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKNHIKNRKGEDKDQLVVLMHEKKQSAEA
                     LDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNLE"
     gene            854..1786
                     /locus_tag="KRH39_003445"
     CDS             854..1786
                     /locus_tag="KRH39_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG8513318.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KINLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            1838..2320
                     /locus_tag="KRH39_003446"
     CDS             1838..2320
                     /locus_tag="KRH39_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8513319.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2332..3201
                     /locus_tag="KRH39_003447"
     CDS             2332..3201
                     /locus_tag="KRH39_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG8513320.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3244..3999
                     /locus_tag="KRH39_003448"
     CDS             3244..3999
                     /locus_tag="KRH39_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG8513321.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNELLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4041..4862
                     /locus_tag="KRH39_003449"
     CDS             4041..4862
                     /locus_tag="KRH39_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HBG8513322.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            4867..5628
                     /locus_tag="KRH39_003450"
     CDS             4867..5628
                     /locus_tag="KRH39_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBG8513323.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIFVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    5789..6046
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6159..6398
                     /locus_tag="KRH39_003451"
     CDS             6159..6398
                     /locus_tag="KRH39_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG8513324.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
BASE COUNT         2828 a          603 c         1104 g         1895 t
ORIGIN      
        1 cttttatagt agttggtgtt cgcgtcagct ctttaaatat gaaggaaaat gaaattagta
       61 gaaatgcaaa actgtcaagt tctactatta cagatatagt atctaataca tcaatagaag
      121 ttagtaaagg gccaagtaaa agttctggaa aaatagcata cataactata gatgatggtc
      181 catctaaatt tactgacgag atgataaaaa ctttaaataa atacaatgtt aaagcaactt
      241 tttttatgat agatgggaat atgaaggaat atccacagca agttaaaaat ataattaaaa
      301 atgggaacac agcaggtttt catagtgtat cacatgatat tcacaagctt tatgttacta
      361 gtacatcagc aaaagaggaa tttgatacaa atgatcaaac tttttataag ataactggta
      421 aacattcaaa agtaataaga ataccttatg gaagcaaacc atatacacca caagcctctt
      481 atcaggcttt agttgatgct ggatataaaa tttgggactg ggacttagat acagaagact
      541 ggagatctaa ttcttctcaa attgtacaaa atgtaaaaaa tcatattaag aatagaaaag
      601 gtgaagataa agaccaactt gtagttttaa tgcatgagaa aaaacagagt gcagaagctt
      661 tagattcagt tttaaaattc ttatcagatg aaggttatga atttgcgcca gtagatcaaa
      721 accaaatacc aaaaaattat tggttacgta atttagagta gttaaaatat aagaatattg
      781 attgatattt ttagaaattg agaataaaaa tgagtgaggt gtaggtatat tgaataaaaa
      841 actttatata ggaatggtcg gaatattgtc tttgatgatg gttggttgta ataaaagttt
      901 agcaaaagta aatgatgtgg aaatcacaaa agaacagtac aaaaaaacaa aagcagtttt
      961 atctgctact aataattata taaatgggca gtctttagat gaattagaaa aaactttgga
     1021 taaaaaaggt agaaataaat tagaaaatgt tataatatcg tttatggtgg ataatgaact
     1081 tttatatcaa gaggctaagg ataaaggact aactccaagt aaaagtgaag tagactcaaa
     1141 atatcaagaa ctagaagata agataaattt gaatacaagc tataaggaaa aaatggacaa
     1201 agcaggtgtt gataaagatt atttaaaaca agaaatatct agagatttag caatagataa
     1261 aaataaaaag gcctttgaag atagaataaa cataagtgat aatgatatgg aagcctacta
     1321 tacaagtcat aaaaaagatt ttaatgtaga agaagtaagt gcttctcaga tacttatatc
     1381 gactttagat aaaaataaaa aagaagtaag caaagataaa aaagaagctt tgaagaagaa
     1441 ggcagataat atattgacta aaatcaaaaa tggagaaagt tttgaaagtt tggctaaaaa
     1501 atattcggat gataaagcaa ctggaaaaaa tggaggacag ctaggatatt ttacaaagga
     1561 tgataaaaat gctgagttta caaaggaagt gttcaaatta aagaaaaatg aagtttcaaa
     1621 tgtgtttgaa actagttatg gatatcatat agtcaaagtt actgataaaa gagaaaggca
     1681 aaaaagtttt aatgagtgtc agagtttaat aagagaatct attttaaatg aaaagtatat
     1741 tgagcatata aaaaaactga atgaagatgc taaaatagac agatagaatt tctaacagct
     1801 caatcctagc ctgtaatcgg agggagattt tacaggaatg tttaagagtt taaagagaac
     1861 gaaagttgag aaatacatta tcgataataa agactctttt tatagaatag catatagtta
     1921 tacgaagaat gaagaagatg ccttagatgt ggttcaagaa gccatgtata aggcactata
     1981 ctcagtggaa aatattaaag aagtaaatta tataaagaca tggttttata aaattttagt
     2041 tagaacgagt atagatttta ttagaaaaaa ccgtaaatat aataatatga cagatattga
     2101 ccttatagat gagactggag agtatgataa atatactgat ttggatttaa gaagagcttt
     2161 ggaagaatta cctattgaat acaaatctat tataatatta agattttttg aagatttaaa
     2221 gattgaagaa gtagcaataa tattggatga aaatgtgaat actgttaaaa ctaggttata
     2281 tactgcttta aaaaaactaa aattaaaaat tgaagagtag ggataaaaag aatgacaagc
     2341 aaagaaaggt taaataagtt aaaagatgag tatcataaaa ttccaatacc caagaaatta
     2401 gatactataa taaatcatga aaaaatagaa aacatatatc gagagaaaaa taaaaaagta
     2461 aatagattaa gatttaaagt tgcaatagcc tttgcatgta tatttacagt attagtaaat
     2521 ataagtcctg tatttgcaga caatttttct aagatacctg taattggagc aatagtagaa
     2581 gtaataacta taaaaaatta tagtttaaaa agtgaaaatt atgaagctga gattgatatt
     2641 cctaaaatac gaggtttaaa agataaaaac ttagaacaaa gattaaacag tagttttatg
     2701 gaagatggaa aaaggcttta tcatcaattc caagagagaa tggagaagat acaatcatca
     2761 aagaataaag gatataaatc tttaagtttg agttatagtg taaaaaataa ttctaaaaaa
     2821 tttctatcaa tagagatgac aaaaaatgag atagaggctt catcatatgt gagtaaggta
     2881 cattatacta ttgataaaaa aagacaaata gttttgactt tgccaatgct ttttaaagat
     2941 gataagtata taaaagttat aagtgataat ataaaagaac aaatgagaga acaaatgaaa
     3001 aaggatagta caaaatccta ttttatagat caaaaaaaag acttgcctgt tgaagatttt
     3061 aaaacaatta ataagtatca ggatttttat tttaataaaa atgaagacct tgtaatttgt
     3121 tttgatgaat atgaagtagc acctggttat atgggcgcag ttgagtttgt aataccatat
     3181 aaagtaataa aagatttgta aattgaatag tagtttatat ataaatcttt taaggaggac
     3241 aatatgacta catataaaaa acttaaaaaa tctatattta aaattgctat attttcaata
     3301 tgcttattag tttcattagt agatacaaac cctatatttg caggtagttc ctttaatgaa
     3361 acttcaaata gtgatacgat aaaggtgtta aatgctaaga gtatcaactt taaacaagac
     3421 aattatgaag tgaatgtgag tattcctaaa ataaaaggtt taaaagataa aactttagag
     3481 aataaattaa acaatgagct tttgcaagat gggaaaaaaa tttacagtga atttcaaaat
     3541 aaaatgaaaa aggaagataa taagggtcat agatacctaa atgtaatcca taatgtcaaa
     3601 aataattcta aggacttttt atcaatagaa atgacaaaag aagagataga agcttcctca
     3661 tctataacta agaagcatta cactgttgat aaaaatagag gaatactatt aaatttacca
     3721 atatttttta aagatagtag atatattaga gctataagtg ataatataaa agagcagatg
     3781 agaaatcaaa ttaagaaaga tgatacaaaa tcttatttta tagaccaaga taaagactcg
     3841 ccagtgaaag attttgaatc tataaataag tatcaagatt tttattttaa taaaaatgga
     3901 aatcttgtga ttagttttga tgaatatgaa gtagctcctg gttatatggg agcagtagaa
     3961 tttgtgatac caaataaagt aataaaaaga ctaagataga tatatagaag atttttagat
     4021 agactggtaa ggagaattat atgaatatat atgaacaagc aataagatta atagaaaata
     4081 atgaggattt cgcatttgct acaataacgt ctcattcagg ttcaacacca cgtgaaactg
     4141 gagcaatgat gattgtgaaa aatgattcta ctatatttgg ctcagtagga ggaggtagtg
     4201 tagaagcaga atgtataaaa catgcaatta atgttataaa aaacagagaa tcaatgttgt
     4261 ataaatttac tctaaacaaa tctgatgttg caaaactagg aatgatatgt ggaggtactg
     4321 gagaaataca aatagatttt atagatagta aattaaaaag taatatagaa aaatttaata
     4381 agagactaaa ggaaaataca agtaaagcat atatatttgg tgcaggtcat atttcaagag
     4441 atgttgctgt gatactatct cttttagaat ttagaacagt agtaatagat gatagagaag
     4501 aatttgctaa tcatgaaagg tttccagaat cagaagtagt agttttagat tcttttgaaa
     4561 acataccaga ttttccaact gacgaaaata gttatataat tattttaaca agaggtcatc
     4621 tatatgattc aagtgcttta gaatgggctc ttaaaagaga ggctggttat ataggtatga
     4681 ttggaagtag aacaaaaatt ggattgactt acgaaaaact catgaaaaaa ggttttaaga
     4741 aggaagaatt atcaaaagta catgcaccaa taggcataaa attgaatgct caaacaccag
     4801 ctgaaatagc tgtatgtata gctgctgaac ttataaattg tagagctaat aaggaaaaat
     4861 aaactaatga acacagggag taaagatatt gatactttaa aggttaattt tagagggatt
     4921 gaaataaaag cagttttagt atataaaaat agaaaaaata tttctataaa aatagatcca
     4981 tttggcaaaa taactgtaat gagtcctcct aaaatatcca aaaaaataat aaaagatata
     5041 attattgaaa aaggagactg gatacttaaa aagtcagaaa aatgtaaagg aagagaggaa
     5101 gtatataaac aaagaatgtt tataactggc gaaaaatttc tttatttagg tgaagagtac
     5161 catctagtta taaaagaact gttgagagat tctgtcaaaa aaagtaactg tagaataact
     5221 attaacgaat atcaaatatt tgttcagaca aatgatacaa gtacagattt tattaaaaaa
     5281 tctttaaaat cttggtataa gatggaaagt gaaagaatag tattagagag aatagatttt
     5341 cttaaattaa attgtgaaat aatgaagcaa ttagttccag cgagtgttaa ggtaaaagaa
     5401 caaaataaga gatggggaag ttgtacagct caaaaaaata tttatataaa ctcaaaaata
     5461 tctatggcaa gattagatgt aattgattat gtaataatac atgagtttag ccatttagta
     5521 catatgaatc actctaaaaa attctatgat ttagtaaaat ccattatgcc cgattataaa
     5581 gataaagaaa attggctaaa aataaatgga tataaaataa taatctgaaa acgattattc
     5641 caaaatatag gcgtaaagta taaaagaagt attattgtga aaaaaataaa taatctgaaa
     5701 attttaaaaa aagtcattga cattgtaaat gatagggaat ataatctata ttaacaacaa
     5761 aacagaatta aaatataaaa ttaaaaaatt aaaagcaatg acagagacaa caatactaat
     5821 agtgttatcc acagagaacc aatgttgctg agaattggtg ttaacagtag tataatgatc
     5881 actctggagc ttttgtactg aatattttta gtaagtacaa acgtatgtca acgttacatg
     5941 actaaggtta aggtaattaa ttacccgacc tgaaaaaaga ggggtagatg tattttttat
     6001 atctacaaat aaggtggtac cgcggaatat aactttcgtc cttataagta atatttatat
     6061 tgcttataaa gatgaaagtt tttttatttt aagcgatata aataaattta atccatataa
     6121 aattaataaa aaaataataa aaataaggag ggatttttat ggaaaatact gtatatgcta
     6181 aattaaacca aggaatagat tcttttttaa gagtggctat gactctaaga agaagagagg
     6241 ttgatataca atcaataagt atgactgtag ataatatgaa taattcaggt ataaaattaa
     6301 tagtaaatga agaaaagaca tccttagatt ctgtcttaaa ccatatgaaa aaactacatg
     6361 atataagaga gataactgtt gaaagaatga gacattaatt aaatatattt tataaagcta
     6421 aagataaata
//
LOCUS       DAEORZ010000186         6420 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEORZ010000186 DAEORZ010000000
VERSION     DAEORZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.190"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            302..1246
                     /locus_tag="KRH39_003452"
     CDS             302..1246
                     /locus_tag="KRH39_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBG8513325.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1268..1894
                     /locus_tag="KRH39_003453"
     CDS             1268..1894
                     /locus_tag="KRH39_003453"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBG8513326.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1928..2977
                     /gene="hisC"
                     /locus_tag="KRH39_003454"
     CDS             1928..2977
                     /gene="hisC"
                     /locus_tag="KRH39_003454"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG8513327.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2980..3558
                     /gene="hisB"
                     /locus_tag="KRH39_003455"
     CDS             2980..3558
                     /gene="hisB"
                     /locus_tag="KRH39_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBG8513328.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3560..4165
                     /gene="hisH"
                     /locus_tag="KRH39_003456"
     CDS             3560..4165
                     /gene="hisH"
                     /locus_tag="KRH39_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBG8513329.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4162..4884
                     /gene="hisA"
                     /locus_tag="KRH39_003457"
     CDS             4162..4884
                     /gene="hisA"
                     /locus_tag="KRH39_003457"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBG8513330.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4866..5624
                     /gene="hisF"
                     /locus_tag="KRH39_003458"
     CDS             4866..5624
                     /gene="hisF"
                     /locus_tag="KRH39_003458"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBG8513331.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMDVEVRL"
     gene            5652..6320
                     /locus_tag="KRH39_003459"
     CDS             5652..6320
                     /locus_tag="KRH39_003459"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBG8513332.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2622 a          540 c         1265 g         1993 t
ORIGIN      
        1 caaaaaactg taaatttaga atattaataa aaattatttg ggaggaaatt atcatggtga
       61 gattaaatat agtaaaatta aatacaaaat taaatcatca ccatcatcat agattaaagg
      121 gattgtaata tgaaattaat tcatagacac aagccctgtt tttagtgtaa ttggtcttgt
      181 atctatgatg ggaagtaatt ttaagagaaa attatagcca tataggtata aagcctatgt
      241 ggctttttta ttgttttaaa tttgcaaata taagagtaaa tttaatgaag agggtgagaa
      301 tgtgaaattt ttgaggattg aagatgaggt tatttattct aaaaaaagat ataaattaaa
      361 aagggaaata gataaacttt ttttggatga agaatatttt caaatagaac cacaattatt
      421 tgaagaatat gatgaattta caactataaa taataaaatt ccagaagaat ctatggtaaa
      481 ggttgtaaat ggaaaagtaa tggtacttag agctgatata actacaagta taataaaaag
      541 ccttgtacca agatgggaag atggtttaaa gcttaagctt ttttataatt catcaatata
      601 taaaaataaa aatacagttg gaataaaaga gataagacaa ataggttgtg agtatctagg
      661 agaagcctct gtagaagcag atagagaagt agtgaaatta gctcttaaga tacttgaaaa
      721 atacaataat aattttattc tagaggttgg gagtagcaag tatattcatg gtttgttaga
      781 agaattaaat ttaaataaga attgtgaaaa tcaaattaaa aatttactat atacgaagaa
      841 cactcatgaa ctaaaagtat atattgaaga attaaaaatt aagcgtgaag taaaagagtt
      901 attatcaaat attttaagtt tacaagggaa tttatataat gttattgaaa aatcaaacaa
      961 gttctattgt aataataaga tgaagcaagc tcttgaagaa ttaaagcaag taaataactt
     1021 gattgaggaa tgtaattttt tagataaggc tagatttgat ttatcaatga ttactatgct
     1081 tgactattat gaaggaatta tgtttagagg atactaccca aactcatata aagaaattct
     1141 tagtggcgga agatatgatt ctttaactaa agagtatgga aaagaaattc cagccattgg
     1201 atttacacta agtgttgatg agttaatgaa gtatgtatat aagtaaagtt tagaaggagg
     1261 gattgttttg gattatttga ctatagcact agcaaaagga cgaattgaag gggaatcatt
     1321 taaaaaattt aaaaaaatgg gtctaggaga tagtatagac acagatacga gaaaactaat
     1381 ttttaaagat gaagaaaata aaattatata tatacatgta aagccatcag atgtagttac
     1441 atatgttgaa aaaggagtcg ctgacctcgg tatagcaggt aaagatacta ttttagagaa
     1501 tgagacagat gtatatgaaa tatatgattt aggttttgga aaatgtaaat ttgctgtagc
     1561 aggattaaag ggagatagca tatatagaga agatgaatat ttaaaagtgg caacaaagta
     1621 tcccaatata gcaaaaaagt attttaaaga gaaaggtcaa aaaattgaaa taataaaatt
     1681 aaatggttct gtagaactag ctccaatagt agggttgtca gatgtaattg tagatatagt
     1741 tgagactggc aatacactta aagcaaatgg tcttgaaata ttagaagaca tatgtaatat
     1801 aagtgctaga attatatcaa atagagctag ctatagattt aaatatgaac aaattcagaa
     1861 tataattcgt ttatttgaag aacttgataa ttaaaatata attgagcttt ataaggaggg
     1921 atttaagttg agagaaaaag aaagcataag agagttaagg ggatatgaac caaatcacgt
     1981 aaactgtaaa gtaaaacttg atgcaaatga aggaagtaag aggttgttta aataccttat
     2041 taaagaaatt tcagatagtg atatagattt aaatttatat cctgaagatt cttatagtga
     2101 tttgaaagaa tctataatag attacataaa tatttctgga gtaaataaga aaaatcttct
     2161 agtaggaaac ggttcaagtg aaataattga tttaataata catacttttg tagataaaga
     2221 tgaagtaata ttgagtttct ctccaagttt tagtatgtat tctatatata gtcagataaa
     2281 tgggtctaaa tttataggtg ttgaaagtga tgaaaattta gttataaata tagataatgt
     2341 gattgaaaaa gtaaaggaga ataatcctaa aatagtaatt gtatgtaatc ctaataatcc
     2401 aacgggaaca atattaaaga gggaagagat aataaagctt ttagattcaa ctaatagtct
     2461 agtggtttta gatgaagcat atatggattt tggagaagaa agcatgttaa gtgatgtttt
     2521 taagtatgat aatctaatag ttcttagaac tttatcaaag gcatttggat tggctggaat
     2581 aagaacagga tatatgttat ctaatagtag tttaataaat tctgttgaga aagtaagacc
     2641 tccatataat ttaaattcat tatcagactt tattgctaca agagcactta gaaataaaga
     2701 tgtggtaaag gcttatataa aggaagtaaa agaggaaaga gaagttttat atgaagaaat
     2761 gattggtatg ggaattaaag catacaaatc acaagcaaat tttatattat tttattctga
     2821 aatcgaaaat ttatcacaaa aacttattga tagaggagtt ttaataagaa aatttggtgg
     2881 aaagttagaa aattattaca gagttacaat tggtgataaa gaagaaaatt ctatgtttgt
     2941 tggagctata agagatattt tgaaaaagga gaaataggta tgagaatttg gaaggtagaa
     3001 agaaatactc ttgaaacaca gattttagta gaattaaata ttgatggttc tggtaaagct
     3061 gaaatagata ctggaatagg atttttagac catatgctta ccttaatgtc atttcatggc
     3121 aagttcgatt taaaagtgat ttgtaagggt gatacatatg tagatgacca ccatagtgta
     3181 gaagatatag gtatagctat aggtgaagca tttaaaaatg ctctaggtga caaaaaagga
     3241 atacgaagat attctaatat ctatatacct atggatgaat ctctatcaat ggtagcaata
     3301 gatataagca atagacctta tcttgtattt aatgctaaat ttgataccca aatgattggt
     3361 agtatgagta ctcaatgctt taaagaattt tttagagctt ttgtaaatga atcgagagta
     3421 acattgcata tcaatctctt atatggggaa aatgaccatc ataagataga aagtattttt
     3481 aaagcatttg caagagcttt aaaagagggt agtgaaatag tttctaatga gatagcatct
     3541 tcaaaggggg ttttgtagaa tgaatataat agtagactat ggtcttggaa atatagattc
     3601 tgtgtctagg ggttttagaa aagctggaat agaaactaaa atttctagtg atatagatga
     3661 aataaaacag gcagattcac ttatacttcc aggtgttgga gcttttagag attctataag
     3721 tgccttagat aaactaggat tgataccaat aataaaggag catgtttcta agggtaaatt
     3781 tatgataggt atatgtctag gaatgcaatt actttatgaa aaaagttatg agtatggaga
     3841 atatgaagga ttaggactta taaaaggaag tattgataaa ctggatataa gtttaaaagt
     3901 gccacatatg ggatggaata atctaaaatt taataaggca aatgatgata tacttaaata
     3961 tataaatgaa gatgattatg tatacttcgt tcactcatat tatgcaaatt catcaaatga
     4021 agaactaatt gcattttcag aatatgaaaa gaaaatccct gctattgtta gaaaaggcaa
     4081 tgtatatggt atacaatttc atcctgaaaa aagtggagaa gtagggttaa atatattaag
     4141 agcatatggg gagatgataa aatgataata tttccagcaa tagatataaa agataataag
     4201 tgtgtaagac tcacacaagg agaatttgac aaagtaaatg tatattatga taatccatta
     4261 gaagtagcat ataaatggaa aaatgaagga gcagaatata tacatatagt agatttaaat
     4321 ggtgctagaa gtgaatttgg tgtaaataca aaaataattg aggacatagc aaacaatata
     4381 gatataccta ttcaagttgg cggtggagtt agggataagg aaaaagtcaa aagtttaata
     4441 aatgctggtg ttactagagt aatattaggc agtatagcga ttgaaaattt gaatttagtt
     4501 gaagaattgg tcaatgaata taaagagaaa attgtagttt ctatagatgc taaagatgga
     4561 aaagtagcag ttaggggatg ggaagttgta agcaatgtag actcattaac attatgtaaa
     4621 caacttgaaa agataggtgt acaaactatt gtttatacag atatatcaaa agatgggatg
     4681 ttgcaaggtc caaattttga catatatgaa agaatagcaa aggaaacatc actaaatgtt
     4741 attgcttctg gaggtgtaac ttctatagaa gacgtaaaaa gactaaaagc tatgaactta
     4801 tatggtgcaa taataggtaa agcgctttat gataaaaaga tagactttaa ggaggcacaa
     4861 caattatgct tactaggaga ataatacctt gtctagatgt gagaaatgga agagttgtaa
     4921 aggggaaaaa atttaaagat atagtagatg ttgatagtcc agaagtactt ggaaaatttt
     4981 atagtgattg tggtgcagat gaacttgtat tctatgatat aactgcatca aatgaagaga
     5041 gaaagacatc tttagaattt gtgacaaaag ttgcagaaaa tataaatata ccattttgtg
     5101 ttggtggagg agtaaataaa ttagaagatt tcactgatat tttaagaaaa ggtgctgata
     5161 aagtttctat aaattcatca gcagtcaaaa atcctgaatt aataagggaa gcttcattaa
     5221 aatttggagc acaatgtgtt gttttatcga tagatgcaaa gaaaaatgaa gagggttcat
     5281 ggagcgtata cgtaaaaggt ggaagagaaa aaactaatct ggatgcaatt gaatgggctg
     5341 ttaaaggtgt agaacttgga gcaggagaaa tcgttgtaaa tagtatggat gaagatggaa
     5401 tgaaaaatgg atatgatata gaattattat caaagataac ttctttagta aatgtacctg
     5461 ttatagcatc tggaggcgct ggaaaaaagg aagattttta tgaggcggtt aataaatcca
     5521 atgtagatgg aattttagca gcatctgttt ttcattttgg cgagataaaa ataaacgatt
     5581 taaagaaata tttaaaagac atggatgtag aagtaagatt ataagttaaa tactaaaagg
     5641 agattagaaa tatggataat aagtgtaaca atgtatacag tgatgaagta gagaaattta
     5701 taagaagtat aaagtttgat gataaagggt tagtccctgt tgttgttcaa gaggtagtta
     5761 gcaaagatgt actgatgtta gcatatatga ataaagatgc tataaagaaa accttaaaag
     5821 ataaagtagc ttgctatttt agtagaagta gacaagaact ttgggttaaa ggtgagacat
     5881 ctggtaatac tcaaaaagta gttaagatgt cttatgattg tgatgtagat actatactac
     5941 tttttgttga acaaactggt gtagcttgtc atacaggcaa ttattcttgt ttttacagag
     6001 acttgtttga tgatagttca aaaatggagc ttgaagtaca aacaaatata ttgaaagaat
     6061 tatatgattt aatcaatgaa agaaaaaata atccagttga aggttcttat actaattatc
     6121 tttttgaaaa aggaatagat aagattctta aaaaagtagg tgaagagagt agtgaggtaa
     6181 ttatagcatc aaaaaatacc gataaaagtg aacttattta cgaaattagc gatttagtat
     6241 atcatacatt ggttttaatg atagaaaaag gtgttgaaat tgatgaaata aaaaaagaat
     6301 tacttaaaag aagaaaatag aataagatag attaatgggt aataagaaaa tataccgctt
     6361 ttaataaaaa gtggtatatt ttttatttta tattttgatt tatagaataa tatttaaatt
//
LOCUS       DAEORZ010000187         6401 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEORZ010000187 DAEORZ010000000
VERSION     DAEORZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.191"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(1..471)
                     /locus_tag="KRH39_003460"
     CDS             complement(1..471)
                     /locus_tag="KRH39_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBG8513333.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            610..1254
                     /gene="uppS"
                     /locus_tag="KRH39_003461"
     CDS             610..1254
                     /gene="uppS"
                     /locus_tag="KRH39_003461"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBG8513334.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            1297..2202
                     /locus_tag="KRH39_003462"
     CDS             1297..2202
                     /locus_tag="KRH39_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8513335.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            2517..4064
                     /locus_tag="KRH39_003463"
     CDS             2517..4064
                     /locus_tag="KRH39_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8513336.1"
                     /translation="MNKNSMTKIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            4067..4465
                     /locus_tag="KRH39_003464"
     CDS             4067..4465
                     /locus_tag="KRH39_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG8513337.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            4505..6238
                     /locus_tag="KRH39_003465"
     CDS             4505..6238
                     /locus_tag="KRH39_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG8513338.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
BASE COUNT         2627 a          591 c         1035 g         2148 t
ORIGIN      
        1 ttattttttt atgatattca gtattttgtt ttctatttga gttgcatttt cagcagaaat
       61 taaataaaca tatctacctg ccttctcaat tatagtatta tttgctattt cagaattgat
      121 tttttctcca tgtccttctc caaaaggtgc ttgtatcata tcttttttat aattttctag
      181 agttttatag attttatcta cctcgtcagt ttttataacg ataatatctt ctagagataa
      241 tttttcagaa gaattaatta caaaaccttg aattatgtct tcctctacat cattaaaata
      301 gttaaaatcc tttgcatcaa agatttttga ttcctttata agtaaatcag aagattttaa
      361 tgcttttttt atttttgatg tagaaacgtt tttgtaatca ccacttgaag aacaactaac
      421 cattcctaat gataagatta tcgaaaaaaa caatattaaa atttttttca tgataaacct
      481 ccaagttttt taaactctgt cttaaattag tttactacat aattatataa aaataaatgt
      541 atttaatgtt acagttttga ttaataatgt aatattttga taataatacc tattgagagg
      601 tgattagaaa tgagaatacc aaaacatgta ggaattatac cggatggaaa tagaaggtgg
      661 gcaagtcaaa atggtatgac aaaagataaa ggatatgaaa gtggaataga ccctggacta
      721 gagtcttata gattatgtag agatttaggc tgtgaagagg tgacttttta tggatttaca
      781 actgataata caagacgtcc atcagtgcag acaaaagctt ttacaaaagc atgtattgat
      841 gctgcaaata tattaataga tgaaggtgct gctttattag ttgtaggaaa tacaaaatct
      901 ccaatgtttc cagaagaatt attaccatat actaatagaa ttaaagccaa agaaggtcaa
      961 ataaaagcaa atcttctggt aaattatggt tggtattggg acttaaataa tatatcagga
     1021 gtagaaaatg taaataaaaa taatatacag gattacatac agtcatttga tgtatccaga
     1081 ctagacttga taatcagatg gggtggaaga aggagattaa gtggtttcct tcctgtacaa
     1141 tcaatttatg cagattttta tgttatagat aattattggc cagattttaa gagtgacgat
     1201 ataaaggacg catttagttg gtatgataca caagatgtca ctttaggtgg ataattaaaa
     1261 atattctgat ttaaggttgt agagtgtttt aaattattga aaaaaaaact tcttgatggt
     1321 aaaataacac taataatatg taaaagtgta aaaatttata ccaagaaggg tgaagaaatg
     1381 tcaaaaggca ataataacaa taattcaaga aacaaatcta aaaagaccag tcatctgaat
     1441 agaaaaaagc gaaaattaaa taagaaaaag ttagcagtgt tgatttgctt tacagtttta
     1501 tttttattta ttgcatttaa ggctactcaa ggtgtagtag ctttggtaaa aagtatggat
     1561 aaaagtaata aaactaatca acaacaaaat gttaactcag aacaatttga ccttggtaat
     1621 gaagaggaaa ataaaaagaa aaaatatact gtatttatag acccaggtca tggaggcaat
     1681 gataaaggaa ctgaaagcaa gaccagtaat agatatgaaa aagacctaaa tctacaaatt
     1741 gcaaaaaaat tagcaaataa attatctaaa caaaaggata ttcaagtagt agtatccaga
     1801 actgatgata catatatatc attaaaggat agagctatct tagcaaataa tagttctgct
     1861 gatgtgcttg tttctattca tttaaatgca gagaaaaatg gtaatacagc aacaggtata
     1921 gaaacttggt ataggaataa agctacagat ggttcaaaag aacttgcaca gacagtacaa
     1981 agtactatag tatcctatgt taaagttaga gataggggaa tagttgaaaa taactttgaa
     2041 gtacttagag aatcaaatat gcctgctata ttaatagagt gtggtttttt aacaacacct
     2101 tcagaggaac aaaaaataat aaatgaaaaa tatcaagacc aacttgcgga aggaattgtt
     2161 caaggtgtac tttcatacct agattctaaa gggaataagt aagttaactt tactttaatg
     2221 tgaggataaa tttaaaagtt taactgtgta aattgtatta actttatttc gtaatgttgc
     2281 aaacattgtt tgaatttaga aactatgaaa ttagaaatta ttttatctat atctaatttc
     2341 atagtttttt ttgcaaaaaa tttatagtta taaaatttta aaaaataaat ttagtataat
     2401 gtaatgtagt tatttatatg tgattttaac ttgcattgat ttgctatcaa ttgtgcttta
     2461 taattagtat atataaagtt taactgtaag ataatttaaa aaatagggga atttatatga
     2521 ataaaaatag catgacaaaa atttacagtt taaataaaat aatttttatt ataattggaa
     2581 ttttaggaat agttttactc acaagacata ttgaggtaga tattaaagtt ttaattttaa
     2641 ttttattaac cttgtttagt ataaatgtat ttatttcata tcttaaaatt aagttgtatg
     2701 aggataaaat taatttagga ttgataaata attcagataa gtttataaat attactaaag
     2761 atgagaactt cctaaaacat gttcaaaatt atattatcag taatgaaaaa gaaaactgtg
     2821 taatggcttg ttttgattta tgtagactca aacttataaa tgatatatat ggttatgagc
     2881 taggggatga agtgttaaat aatattttaa gtaacttaaa aaattatttt ggaaaagaag
     2941 ctatttatgg aaagttaaaa agtgatgttt ttgtattaat aataaagctt gaaaataggg
     3001 aacagaagat tccttattta gtaaatctta ttaagaataa aattgtgata ctaagtcaga
     3061 ttgacagttt taaaatggat ataaatattg aggcttctat tggaatttat aaatttaaca
     3121 ataatgaaat tgatataaaa aaagctatag ataatgctga tatggcaagg cttaaatcaa
     3181 aaggactaaa gcacatagag tatgtagaat ttgataatgc tatggaagaa gaaatacaat
     3241 caattatgaa aatagagaga gatttattct tggctattaa aaataaagag tttgtaattc
     3301 actatcaacc taaagttgat tcaagtacag gtaacattat aggttcagag gcattgataa
     3361 gatggttgca cccatcatta ggcatggttg gaccaaataa gtttatacct atagctgaaa
     3421 aaaatggtct gataaacaat attggtagat ggctaattca agaagttttt attaccatta
     3481 acaagtggat ttcagaaggt ataaatgtct taccagtctc aataaatctt tctagagtag
     3541 aactatataa aaatgattta gttgattttt ttaaactgat gtttaataca tataatattc
     3601 caaaagaact tatagaatta gaaattacag aaacgacagc actaagagat gtaaaattta
     3661 taagtgaaag gctttatgag attaaatctt taggtatgaa tatatcatta gatgattttg
     3721 gagtaggtaa ttcaaatttt atcaatctta aggggatacc actagatatt ataaaaattg
     3781 ataggtcact tatattggat atagttacaa atacaaaaac aaagtttatg gtgaaagcaa
     3841 ttgttaacct ttcacatgat ttaaacgtaa ctgttatatg tgaaggggta gaggatatgc
     3901 atcaggtcaa agtcttaagt gaactgggat gtaatttcat acaaggttat gtatttttta
     3961 aacctttgga tgagatgaat tataaaaaac ttcttactga cggaagttat ataaatctga
     4021 aagatactct tttagataaa tgcatgacag ttgaggagga gtaaggatga tttcagaaga
     4081 tgtagattat tcaataagaa tcataagagc tatatttaat aatggagaag ataaaccaat
     4141 agatgctaaa atgatattaa aaagtgaaga tataccaata ctaacactat acagactgct
     4201 tagaaagctt tgtgcaaaca atattcttac ttttaacaaa gaagatggca agacatacat
     4261 gttaaatgtg tcattggata caataagtct aagaactatt gtagaagtaa ttgatggaaa
     4321 aatatatata aataaatgta cgagatatgt atctaattgc agtaattgct taaaaggttg
     4381 taaagtaaat cgtgaattgc tagaaataga aaaagaaatt tataaacttt tatcgagaaa
     4441 atctataaga caaatactag aataaaaaat tttcagctat gataataatt ggaggttttc
     4501 ggttatggat gatatatctc aagataattt tctactgtct aaagagtatg agaatagctt
     4561 agatgtagat actaaaaaag caagtggaat atattatact cctaaaataa ttgtagatta
     4621 tattgtaaaa aaaactttaa aaaatcacga tataattaaa aatccatatc cacgaatact
     4681 tgatatttcc tgtgggtgtg gaaattttct tttagaagta tatgatattt tatatgactt
     4741 atttgaggaa aatatatatg aattgaaaaa gaagtatgat gaaaattatt ggactgttga
     4801 taacatacac cgtcatatat taaactattg tatatatggt gctgatattg atgaaaaagc
     4861 tataagtata ttaaaagata gcttaacaaa taaaaaggta gtaaatgatt tagatgaatc
     4921 tgatataaag attaatctat tttgttgtga tagtcttaaa aagaaatggc gatataagtt
     4981 tgattacata gtgggtaatc caccctatat tggtcataaa aaacttgaaa agaaatataa
     5041 aaaatttctc ctagaaaaat atagtgaagt ttataaggat aaagctgact tatatttttg
     5101 tttttataaa aagattatag atattttaaa gcaaggaggg attggcagtg ttataactcc
     5161 aagatatttt ttagaaagtc tatcaggtaa agatttaaga gaatatatta aatcaaatgt
     5221 caatgttcaa gaaatagtgg attttttagg tgcaaatata tttaaaaata taggggtatc
     5281 tagttgtatt ctaacttttg ataagaaaaa aacaaaagag acatatatag atgtatttaa
     5341 aatcaaaaat gaggatatat gtataaataa atttgaaaca ttggaagaac ttctaaagag
     5401 tagcaaattt gaacatttta atataaatca gagattgcta tctgacgaat ggatacttgt
     5461 aaataaagat gatgaaactt tttataataa gatacaagaa aaatgtaaat atagcttgga
     5521 agatatagca ataagttttc aaggaataat tacagggtgt gataaagcgt ttattttaag
     5581 taaagatgat gtaaagttga accttgtaga tgataaattt ttaaaatgtt ggataaaaag
     5641 taagaatata aataaatata tagtagataa aagtgaatat aggctaatat actcaaatga
     5701 tatagataat gagaatacaa ataaaaggat actagatgaa attatagggc tatataaaac
     5761 aaaattagaa aacagaagag aatgtaaaag tggaataagg aaatggtatg aacttcaatg
     5821 gggtagagaa aagcttttct ttgaaagaaa aaagataatg tatccttata aatcaaatga
     5881 aaatagattt gctatagatt atgataataa tttttcaagt gcagacgtat attctttttt
     5941 tataaaagaa gagtatttag ataaattttc atatgaatat ctagttggaa ttttaaattc
     6001 cagtgtttat gataaatact ttaaaataac tgctaaaaaa atgagtaaaa atatatatga
     6061 ttactatcct aataaggtta tgaagataag gatttttagg gataataatt atgaagagat
     6121 agagaattta tcaaagcaaa taatatcaat tttattaaat aagtctatag ataaaggaaa
     6181 agtagaaaaa ttgcagataa aaatggataa cttgataatg gattctttag gtatataagc
     6241 agttgtaagt attaaaaaat tataatcttt taattgttta tttaaattta gaaataaaaa
     6301 gttagccata taaaaaatat taaaaatata tacttttcaa tatttttttg ataaaataag
     6361 aatgaatttg tgcaatttta tttttctcca ataactaaaa a
//
LOCUS       DAEORZ010000188         6375 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEORZ010000188 DAEORZ010000000
VERSION     DAEORZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.192"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(46..1143)
                     /gene="ychF"
                     /locus_tag="KRH39_003466"
     CDS             complement(46..1143)
                     /gene="ychF"
                     /locus_tag="KRH39_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBG8513339.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            1459..2724
                     /locus_tag="KRH39_003467"
     CDS             1459..2724
                     /locus_tag="KRH39_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG8513340.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRKLYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            2739..4025
                     /locus_tag="KRH39_003468"
     CDS             2739..4025
                     /locus_tag="KRH39_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG8513341.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            4419..5165
                     /locus_tag="KRH39_003469"
     CDS             4419..5165
                     /locus_tag="KRH39_003469"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBG8513342.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            5195..6130
                     /gene="rsmH"
                     /locus_tag="KRH39_003470"
     CDS             5195..6130
                     /gene="rsmH"
                     /locus_tag="KRH39_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBG8513343.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
BASE COUNT         2481 a          662 c         1085 g         2147 t
ORIGIN      
        1 tttagttatt atatataata gtcaaagtgt atacgattaa tcattttaaa cattaaatct
       61 aaataatatt acatctccat ctttaactat gtattcttta ccttctaatt taaccaaccc
      121 tttttcttta gcagcagcca tagtaccatg ctcaaccaaa tcattaaagg ctattgtttc
      181 tgctctgata aatcctcttt ctatatcaga atgtattttt cctcctgcct gaggagcttt
      241 acttccaact tttatagtcc atgctctaac ttcttttgga ccagcagtta agaatgaaat
      301 taaaccaagt aaagcataag aagattttat aagcttatca agccctgatt gttcaagacc
      361 taatgtttct aaaaactctt tcttttcttc agcagaatca agttcagaaa tttctgcttc
      421 tatttgtgca catactacaa caacttcggc atcttctgtt tctgcaaatg ctttaacttg
      481 ttgaacatat ttattatttt ctccattatc agctaaatca tcttctgata catttgaagc
      541 atatattaca ggcttagatg taagtaaatc caatgaattt acaaatgttt gttcatcttc
      601 tgtaaattcc atagttctta cacacttact ttcttctaaa gtagacatta ttgattttaa
      661 taaatcaagt tcagaaccta aagttttatc agccttagca gctttttgag ttttattaat
      721 tcttctttca agaatctcta tatctgagaa aattaattct aagtttatag tttctatatc
      781 acgaagtggg tctacagaac catctacatg tacaacattt tcatcctcaa aacatctaac
      841 aacatgaact attgcatcaa cttctcttat atgtgataag aatttatttc ctaaaccttc
      901 tcctttagaa gctcctctaa ctaaacctgc tatatcacaa aattctatag cagtaggaac
      961 tattttttca gaattatata attcttgtag tttgtttaat ctttcatctg gaacagatac
     1021 aactcccaca tttgggtcta ttgtacaaaa agggtaattt gcagattctg cacctgcttg
     1081 agttattgcg ttaaatagtg tgcttttacc tacatttggt aaaccaacta tacctaattt
     1141 catattaatc gtcctctctc ttattctttt ttatattctt tatataactt taaacaatca
     1201 atctatagga ttgcaaaatt gttttaatta tagacataca tattgattat acatcatata
     1261 tatcaagctt gtaaactaga ctttaaagca tattgattta aataatattt gtatttaatt
     1321 taaataaaaa tattgctttt agaaaataat aaaaaggata taattaaata gaaggttttt
     1381 atatgtgagt atatgtaatt aaaaaataaa taaatttcaa ttttaaaatt gatgtttaaa
     1441 taaaaatagg aggtacatat ggttaatact aaaaaaataa taaatttagg taataatatt
     1501 aatcttactt tgataaaaac agaaaagttt aaatcgaatc ttataagttt atatgttcag
     1561 agactattgg atgaaaaaga aactacaaaa aatgctctaa taccatcaat aatagcaagt
     1621 ggaagcgcta aatatcctag tgctagagca atatccaata aattagatga tttatatggt
     1681 tcatctatgg gagctgatgc tgtaaaacgt ggagaacgtc aagtattgag ttttaaagta
     1741 attaacataa gtgaaaagta tttagatgag agtatatttg aagaagtagt agaatttttt
     1801 aatgaagtaa taaataatac gcttgttgta gatggtgggt ttaaagaaga atatttaaat
     1861 attgaaaaag agaatttaag agaaaagata caatcaataa taaatgataa aaaggaatat
     1921 gcgcaagata aatgtattga ggctatgtgt aaaggagaga gatatagtgt aagtgaattt
     1981 ggttatgaag atgaaataga tagtataact tcaagaaagt tatatgaaca ttataaaaac
     2041 atacttaaaa catcacctat agatatagtt gtagaaggta attttgatga agataaagtt
     2101 gtagatataa taagtaaaaa tctaaaattt gaaagagaag aaattataaa tataccaaga
     2161 gcagatttta taaaaaatgt ggatgaggta aaagttatag atgaacaaat ggaaataact
     2221 caaggtaagt tagttatggg ttatagagca aatgtggatt atgcagacat agataaatat
     2281 tatgctttag tagttgggag caatgtctta ggtggtggtc cacactctaa attatttgtt
     2341 aatgtaagag aaaaagaaag tttatgttac tatatattct cttcaataga gaagtataaa
     2401 agtatcatgt ttatatcatc tggtatagaa actaaagatt atgataaagc tgtagaattg
     2461 ataaaaaaac aagtagaaag tgtaaaagca ggtgatatat ctgatgaaga attagaaaac
     2521 agtaaacttg ctttagttaa ttctatgaaa tctataactg ataatatagg tggtatgtct
     2581 gattttgttt tctctcaatc tatggcaaag acaaattctg aagtacaaga tataatatct
     2641 agtatagaaa aggttacaaa aaaagatata gttgaagcta taaaaaatat agagcttgat
     2701 actgtatact tcttgagaaa ttaatggagg agatgtctat ggaaaaaata gttaatgata
     2761 tattgaaaga agaagtttat tatgagaaat tgcaaaatgg attagatgta tattttatgc
     2821 ctaaaagggg ttttatgaaa aagtatgcta ttttggctac taattatggt tcaaatgact
     2881 tagaatttgt accaattgga gaagataaga aaattagagt gaatgaaggt attgcacatt
     2941 ttctagagca taagatgttt gagcaacctg atggaggaga tgcatttgat aaattttcaa
     3001 aattaggagt aaatgcaaat gcatttacta attttacaat gacagcctac ttattttctg
     3061 ctactgaaaa tttttatgag agtttagaac atctaattga ctatgttcaa acaccttatt
     3121 ttacagatga aaatgttgaa aaggaaaaag gtataatagc acaagagata aaaatgtaca
     3181 atgatgaccc tgattggaat gtatacttta attgcttgaa ggcaatgtat gtaaattatc
     3241 ctgctagaat tgatatagca ggtactgttg atagtatata taagataact aaggaagagc
     3301 tatataaatg ttataatact ttctataatc caggaaatat ggctttattt gttgttggtg
     3361 acttagatgt agaaaaagtt atagatgtaa ctaaaaagtc aaacaattac aaagtagaca
     3421 aactctctaa gagtatagaa agattttatc cagaagaacc agaaggtgta aaagaaaaag
     3481 aggttataga aaaattccct atatctatgc ctatgttcaa cataggtttt aaagatagta
     3541 atgttggttt aaaagggaaa gaacttttac gtaaagagat tgtaactgat atacttgtag
     3601 ggatgttatt taaaaaagga agtaagcttt atgaagattt gtatatgcaa ggtttaataa
     3661 atgaaaattt tggagctgga ttttcgtctc aagtggatta tgcttttagc ataatagctg
     3721 gagattctaa agagcctaaa aaagtaaaag aaataattct tgattatata gaaaaatcaa
     3781 aaaaagaagg attatcaaaa gaagagtttg agagaactaa aaagaaaaaa atagggtcat
     3841 ttattaagtg ctttgattct ataaacttta taggtaatag ttttatatct tatgttttta
     3901 aggatataaa tctattggat tatttagata ttattaaaga tattactttt gaggaagttg
     3961 aagaaagatt aaaagagcat tttaaagaag agtattgtgt tatttctatt gtagagccta
     4021 aatagaaact taatcataat aagaataact aattaagtat tagtttatgt ttttaaatgt
     4081 ttattttggt aaaacaaatt tataaaatat atttatataa attgaggtaa aaatttaaga
     4141 gctatagata agaaatagaa gaggttgatg tatatatgca tcaatctttt tgattaaaaa
     4201 atatctatgt acacttgtaa ctttagtcat gagagattca gtttaaaaaa ttttatagta
     4261 attagatata atatagtgta gggagttgta gaataaattt taatattttt tgtagaatta
     4321 acattaatgt gttatcataa tttagtataa ttacaattaa ttgtaattat agcatattgc
     4381 tttggaaata ttttcaatta atacaaagag gtgacgttat ggatagagta gcatttacac
     4441 tgtttggcat agacatcatg tggtatggaa tacttatggc atgtggaatg attttaggaa
     4501 ctttaatagc aataaaagaa gcaaaaagag tgggaattaa agaagatgat gtattaaata
     4561 tagctataat agcaattcca gttggactaa tctgtgcaag aatctattat gttgtattta
     4621 attggagtta ctatgctcaa aatatgtctc aaatttttaa ttttagaggt ggtggccttg
     4681 ctatacatgg agggttaatt ggtggaatat tagctggtta tatatacact aagattaaaa
     4741 atattaactt cttaaaaatg gctgatacag ttatacttgg aatgccttta gcacaagcta
     4801 ttggaagatg gggaaatttt attaatggag aagcacatgg aggtgctacc aatttacctt
     4861 ggggaataat ggttgatgga gtaaaggtac atccaacttt tttatatgag tctatatggg
     4921 attttggaat atttatagtt ttattactat ttagaaaaaa taaaaaatat gaaggtcaag
     4981 ttatagttac ttatataact ttatattcaa taggaagatt ttttatagag ggattaagaa
     5041 cagatagttt aatgttagga ccacttagaa tggcacaagt tataagtcta attggagtta
     5101 ttggtggcat aattgcacat gtttatttat caaagaaaaa taaaaataat attagtgaag
     5161 aatagtttaa aaagtgaatt taagggaggt aactatggaa tttcatcatg tatcagttct
     5221 tttaaatgag tgtatagaga atttaaatat aaagccagat ggcgtgtatg ttgactgtac
     5281 tatgggagga gcaggacatt ctaaagaaat agtaaaaaaa ctgtcagata agggattatt
     5341 tatagggttt gaccaagata aaaatgctat aagcacagca aaagagagat tgagtgaata
     5401 tgaaagtaga gtgaagtttg ttcacagcaa ttttgaaaat ataaaagagg aattagaaaa
     5461 aataggagtt tacaaaattg atggtgtatt agctgattta ggagtttcat ctcatcaact
     5521 tgatgaagct gatagaggat tttcttatat gcaagatgca ccacttgata tgagaatgga
     5581 tgttaggtgt gagttttctg catatgatgt tgtaaatact tatacagaag atgaattaac
     5641 taagataata aaagattatg gagaagataa ctgggctaag cgtatagcta aatttatagt
     5701 tgaagaaaga gcaaacaaac caatagaaac tacaggagaa ttagtagatg taattaaaaa
     5761 agctatccct aagaaagcta gaatagatgg acctcaccca gctaaaagaa cttttcaggc
     5821 aatacgaata gaagtaaata atgaacttgg agtaattaca aagatgataa atgatgcttc
     5881 ttctataatg aatgaaggtg gaagaatatg tataataaca ttccattcat tagaagatag
     5941 aatagtgaaa aatgcattta agcatttagc atcagattgt atatgtccac agcatttacc
     6001 tatatgccaa tgtgataaag aatctgaggt gaaaataata actagaaaac ctatattacc
     6061 tagtgaggaa gaaatagaag taaatccacg ttcaagaagt gctaagctaa gagtagcaga
     6121 aaaaatataa gtagttataa ggctaaagaa taatcttata tataagatta ttataattaa
     6181 ttaaaaagta ttatatattt tgttgcactg gtaaagcaat taaaaaacaa ttaacactat
     6241 aagaaatgtt ctttgttatt gagatatata atgaaaattt agatataaaa atatagatat
     6301 agatggtatt aagtaatata atggagaact taggaatcag agagtagata gttagttaca
     6361 tattatattt atgga
//
LOCUS       DAEORZ010000189         6253 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEORZ010000189 DAEORZ010000000
VERSION     DAEORZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.193"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..2015)
                     /locus_tag="KRH39_003471"
     CDS             complement(<1..2015)
                     /locus_tag="KRH39_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG8513344.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFLYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(2286..3062)
                     /locus_tag="KRH39_003472"
     CDS             complement(2286..3062)
                     /locus_tag="KRH39_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG8513345.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(3119..4090)
                     /gene="asrC"
                     /locus_tag="KRH39_003473"
     CDS             complement(3119..4090)
                     /gene="asrC"
                     /locus_tag="KRH39_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBG8513346.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAIVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(4103..4894)
                     /gene="asrB"
                     /locus_tag="KRH39_003474"
     CDS             complement(4103..4894)
                     /gene="asrB"
                     /locus_tag="KRH39_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBG8513347.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(4895..5902)
                     /gene="asrA"
                     /locus_tag="KRH39_003475"
     CDS             complement(4895..5902)
                     /gene="asrA"
                     /locus_tag="KRH39_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBG8513348.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNTFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
BASE COUNT         2054 a         1136 c          554 g         2509 t
ORIGIN      
        1 gctgcttctt ttattgaaac tttagttata ttacttattc tatacatttc tttgacacca
       61 tttagagttt gcagtaagtt tagacatatt atcttgtcac ctagactttt agtatttaca
      121 atactctcta aaattacact tcttccttca tcatacttat ctacattgta atttgaaata
      181 taattagtat ctctcaatat tttttcaaca tcttcatctt ttattttatt tttattaaca
      241 tttatatata gattatacat attacttatt agtaatttct gattttctaa aaaatcctct
      301 tgatgtttat ctttatatat agcatttatc aacataagtc tatcttcaat aagtgatggt
      361 atcaataata aattttttat atagtaaaca tcagctacat tttctataga cttattgact
      421 tcatcaatta tatttttaac tattccttta tacatttcta taacatgttg atagcctgtt
      481 ttagcatcat atggtaatat aaacttattt actattaatg caataattgc tcctaatgct
      541 acatatgaaa tcctgcttat ggttaaaaca tttgggtctc ccataactac tgctgaacca
      601 agagctgaaa cagttacaca taccattaat tttctataat caactacata agagtttata
      661 taccctgcta aaagaactat taacgacctt aatgtactat cttttattat tgaaaataat
      721 acaataaata ttacagctcc tataaatgta ccttctattc tttttctaga cctttgtata
      781 caattttctg cgtaaggttg tgttaatgaa aagacagtta acattatcca tcttccatct
      841 cttaaatgga aataatccat aataaaaccc gctacagctg tagctaaagc aatctttact
      901 gcataagaaa atcttataga atttaaatta aagtttttct tatatattga tagtcgttta
      961 aattcatgag gtatctttga ttttctttca gctttctttt cgtctcttga ttttactttt
     1021 ttataatcta ctaaaaactc atataaattt tctaaaacac tataaatttc ttttaaattt
     1081 atatttttag tatcctgagc aataacataa tctctaaatc ctaatatctc tttatcagtt
     1141 atggtatctt tattttctaa acacatctta atatagttta tttctttcgt tgctacaact
     1201 aaaacatctg atttactatt tatgctactt tcttctaaaa tattttttat atcttcatct
     1261 tcaaattctt tactatcctt tttgtatctt tccaggatta aacttattct ttctaatgaa
     1321 aataaaatat tcattatgtc tatacctcta atactgatat aaaagtcatc ttttctatta
     1381 tcaaaaataa ccattttcaa atttttagat atagaattta ttctataatt acagctatct
     1441 atttttctca tactagattt aattatagaa ctatcatcat taatagtatt ttttaaaaga
     1501 ctaatttttt ctaataattc atcacatata gatatcagtt ctttatctcc gacatttttc
     1561 atcctatttc tattcataac aaattgagca atcattatta taactgctcc aacagctaaa
     1621 gccgacaatc tcataggaaa ttcacttttt cctacaggta tacttaccat aaatatatac
     1681 attagaccaa agggtacata tactgctctt tttaagtcat aactaaatac ataccctatt
     1741 ataaataatg ctataaaatt taataaaact cctaaataca tattttgtac tgcaaaaaat
     1801 gatagtattc ctaagattat atttattgct aaatacttca gtaaattttt aattggagaa
     1861 atagttaaat ctctttccaa gagtgttagc atagctgtta ctatacctac acctataagt
     1921 gtatttttac ttccaaataa atatagaaat atagaaataa atgctattat aaaaataaac
     1981 aactttgttt ttgatattac tagtttcttt gtcatcatat cccccctaaa aatttcaaca
     2041 agaccaattt tatcatattt gttatcttat tcaaaatgag cttttttacg gtaggacttt
     2101 aacttttttt atatttagaa ctatataatt catatataaa cgtatttttt atatatataa
     2161 tcgatatatt ttcgtatgct cccatatttt ttttgtttca aatatttttt caaaaatata
     2221 aaaataactc tctcaataat actaaaaatg catttatcaa gagagttata atttatacaa
     2281 taaatttaat taagcttttc tttacctaat atataagttc caagacccat aaataaagct
     2341 cctccaacaa tatttccaag tgttacagct actaaattat aaatagctcc tccaatagta
     2401 actgtactta ttgttggtga aaataatgaa acactataaa ttgtcatatt tgctacacta
     2461 tgctcaaagc ctgatgttat aaaagcaaat aaacataaga atatcataat tatttttgca
     2521 gtatcactat tagttctaaa cgaacacaac actgatacac atactagaat attacataaa
     2581 atccctctaa agaaaagagc tgtaaatggc atggatgctt tactagctgc tgtatttgca
     2641 aaaaattcag ctacaggtcc tttgtctact agaccagttc ctacaaatat aatgcctaaa
     2701 accaaagctc cgattaaatt acctacccaa ctgtatgccc atattttaga agtatctttt
     2761 atacttacac ctttatttaa cataccagca gacataacca tattgtttcc agtaaataac
     2821 tctgttcccg tcattataac caaacttagt gctattgcaa atgataaccc cattactatc
     2881 ttagtcatag gtgaacctgc atcagtcaaa agtcctccaa tagtaaatat caaaagtata
     2941 cctataccta cataaagtcc tgcaaacgca gaagatacca aatattttac tttacttgtg
     3001 tttaataaat ttatcttatt tatagctgca ttagttaatt tatccaaagt ttctttatgc
     3061 ataattccct cttccttcta ttaaatttta atttattcaa atatatgttt tactcaattt
     3121 atagatattt aattccactc cagtatatgt tttcgtacat tttagttatt tctggaagct
     3181 ctacaccttc aagtgcccat tttttaaatt ccataaatcc tgttctatca attatatatc
     3241 ctatatgttc ttttcctcct ggtgcatctt tatctatata cttttctaca tatctataag
     3301 tattaagtat tatctttatt atgctgtcct catctaccca taaaagaaaa tcttctgcta
     3361 gtctagggtt tttctttcct gttctaccca ttatagctaa tctatagtat tttttctcat
     3421 ctctagtcca tgcatttgtt gggcaattta gtacacactc tccacagcct atacacttat
     3481 ctttatctct taccacctta ttattttctg tttttaatgc tccaacagat atcttagaac
     3541 atttttttac acatgctcca caatttacac atctatcctt ttcatatatt ggtaaagcca
     3601 taccaataat tccaaagtca ttcattctag cttttataca atcatttgga caaccagtta
     3661 aagccacttt aaaatgtaaa tcatttggaa atatagcttt ttctattctt ttagcaaatt
     3721 cagttgtatt atattgagcc tttggacaga ctttatttcc tatacaagct gctatatttc
     3781 ttgtacctgc cgctggataa cctgcgtttt tctcactttg atttatattc attttttcaa
     3841 taacaggttg tataatttta tttatttctt ccatatcttc catatttatt cctaatattt
     3901 caaacccctg tcttgtagtt atgtgcacct gtccattgcc gtactcactt gcaattttag
     3961 aaactatagc taaactttca gggtcaataa gaccaccagg tactctaact cttaaagaag
     4021 tctcatattt tgtttttgta attctataag catttttcat tacttttttg gtatttaaat
     4081 ctcttatcac aaacttcgcc ccctaatcaa taagattttt agcatatgaa taatcaaata
     4141 ctggtccatc taaacatatg taagtatcat ccattttaca atgtccacat ttacctattc
     4201 cacaacacat ttttctttca taagaaaccc aaatgttctt ttcatctaaa tctcttttta
     4261 aaaattcttc tactgtaaat ttcatcatca ttggaggtcc aactacaact atagaagtat
     4321 tgtttatatc tttcatatcc aattctggta tgtattttgt tactaatcct acattaccac
     4381 tatacccttc ctctgctcca tctacagtaa ctaaaatatc aagtttttta ctccatctct
     4441 ttaaatcatc tgcaaataaa atatcttttg gtgatttaaa tcctactatt aatttaaact
     4501 tttcgcattt ttctttatta ttataaaaat attcaactat tcctcttaca ggggctaatg
     4561 cacttcctcc tgccactact acaatttctc ttccttcata aagggataca tcaaatccat
     4621 ttccataagg acctcttata aaaaaagaat ctccttcttt ataattaaac aactcgctag
     4681 ttacttttcc tacatttctt attgtaaagt caatataatc ctctccatat cctgatactg
     4741 atattggact ttcaccgtat ttaggtatag atatctcata aaactttcca ggcaatacac
     4801 cttttgtatt gcagtttact ctaaaagtcc attctttatc agtatgttta actatatcta
     4861 gtatttttgc acttactggt atatatgggt tcatttatac ttcctcctta ctaattacta
     4921 aatcatttat tttttctata cactttgaaa atgatatgta ttgcggacag gcatcatcac
     4981 atcttccaca accaacacac atatgatatc cgaatctttt tttaaaatca tgtattttat
     5041 gcatagtttt aaatctcatt ctttcacctt gtttttttct aaaagaattt ccacctgcta
     5101 tatctgtata tccatctact tgacaagaag cccaaaccct tcttctctca cctacatttt
     5161 cgttttcctt atagaaaata tcctgcattg taaaacatga acatgttgga catacaaaat
     5221 tacatctacc acatcctata catcttgtat catattcatc ccatattgaa tttcctatca
     5281 tttcatttga atcaatattc tctggcacat ctacagatat taaattatca gttacataat
     5341 caacattaaa ttcagttgct tctccattaa atacttcaaa ttcactatcc tttatatcta
     5401 agtacagtgt atctccttct atatttaatc caattgaata attatcagtt ttgttagaat
     5461 ccatgctcac acaaaaacag tttctaaaac tttccttaca ccctacaagt gcaaacttta
     5521 ctttatctct gatattttta taaaaataat ctttttcttc accattattt agatatattt
     5581 catcaagtct ttttatacca tttatgtcac aagctcttaa gaaaattaaa attttcttgt
     5641 tgtctaaatc gctttcctta aactcttttt cagtaaaata aaacaatact tgatttatgg
     5701 gaagtacaac ttcttttggt gaaaagtttg ctttcttagc aaattccatt tcttcaaaag
     5761 tgtttacctc cttgtatctt ataacatcag tatctgaaaa agtaccttta aatgggatta
     5821 aaactggtgc aaatatcttg tagtcttttt ttagataatt cagaccttca ttgaacttag
     5881 atgaatctaa ttttagtttc atttttatca cctttcttat attttagttt tacttacttg
     5941 ctatcttgtt taaatcttaa cactttattt tctatgcttc cgtgataatt atcacagttt
     6001 caaaaataat aaaaaaaata ttaatggagt ttattaagtt ctgttaatat ttttttatta
     6061 tctacattat tcgatttcaa atctaatcac ttaaatacaa tcattaggta caataaaact
     6121 actatctact caattttttg aataaaaaag aatctcattt gtttttttat acaattcaat
     6181 agggtaaaat aaaaattaat aaattagaca ataaaaaaga atctataaat agattctctt
     6241 aataatattt tta
//
LOCUS       DAEORZ010000190         6073 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEORZ010000190 DAEORZ010000000
VERSION     DAEORZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.194"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            134..1333
                     /locus_tag="KRH39_003476"
     CDS             134..1333
                     /locus_tag="KRH39_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513349.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILSSKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            1342..2154
                     /locus_tag="KRH39_003477"
     CDS             1342..2154
                     /locus_tag="KRH39_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513350.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            2165..3432
                     /locus_tag="KRH39_003478"
                     /pseudo
     CDS             2165..3432
                     /locus_tag="KRH39_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3798..4979
                     /locus_tag="KRH39_003479"
     CDS             3798..4979
                     /locus_tag="KRH39_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG8513351.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DVIVAATKTASELLKIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(5121..6038)
                     /locus_tag="KRH39_003480"
     CDS             complement(5121..6038)
                     /locus_tag="KRH39_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513352.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNDRYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
BASE COUNT         2472 a          590 c          950 g         2061 t
ORIGIN      
        1 attataatct tataggattt caatttaaca atttagtagc agggttatta agttttttaa
       61 ttataatgtt tattctaaat ggaatatctt atttgctaat taaaaagaaa gtatctcttt
      121 aaggaggggt ggtatgaaat taaaatctaa aatatctata cttattatat ttttgacttt
      181 agcaatatat tttagtatag gtttattaaa taatgaagat aatttaaaga taaaaactat
      241 aaaaggtgac tctaatgaat tagaccatat aaacataaat tttggatatg ctttgaatgg
      301 atatataaat tcatattata caataaatgg aagtaacaaa accaaaaaga ctgaattaat
      361 aaaactacaa aaggatagta agaacacaaa taatttaatt gatgtatcta gtggttcaga
      421 tttaattgct catgttggat ttgaaatgaa tgaggagtca aaagatataa actttgtaat
      481 agatgagaaa aaagaagatt tagaaaaagg tgtatttccg tcagaaggta gtgataaaga
      541 aattgaatat gaaaaattag atatgaataa ttttacaagg acaagatata aagtagataa
      601 aagtacagat gattattatg caaagatact atcatcaaaa agatataaaa aatacttata
      661 tgtatctgta ctttatgcta caagtgattc atcagaaaat aacaaagaat attcaagtac
      721 tatagatata ataaaaatta atccagaaaa taaaaaaatc aagactataa aaagtataaa
      781 tttaagtaat tatttttccg aaaaatacca aaacatagag aacataggtg taagttatgc
      841 tagcaaatat gaggataaga tgtatatttt agtaaatgta ctaaatacaa acataggtga
      901 taatagtaat tctaaatttc atttacttga gtataatctt gaagaaaata aggaaagcat
      961 aatggaatta aaaactgata gcaaaatggc tttagcaaaa gcaaagttag aagctaataa
     1021 attatatatg gtaatgtcga atgattatga taatagttca attacagagt taaatttact
     1081 tacctataat atattagata aaagcttaga tgaagataaa tatgttttag ataatagaga
     1141 aaaaagagga attagaagta ttgaaattga ttcaaataaa atcgttgcat atacatcaag
     1201 gctaaactct atattatctt gggataataa atttatgcta tatataataa ataaaaattc
     1261 taaggaaatt gtatatgaag gagaattaca atttaacagt tcaaatagta tgttagaatt
     1321 tgaaataaag taggtgagtt gatgaaagat ttaataagat tattcgaatt tgaattaaaa
     1381 agaaatataa agaattatat atttataata atttgttgtt gttcttttgt aattttaaac
     1441 atagtaaaaa atttaaatga ctataattac ataatagaaa atgcagttaa aagtgaacag
     1501 ttaacaaaaa tcggaaatac gatagaaacg gtaaatgtta caggtttctc aagctttaga
     1561 aatataatgg gttctacaga atcgtggttt atgtttggta taattgcatg tatttgctat
     1621 gcttttttta tttggtatag agactttaat ggtagaagta aatctatata tacacttata
     1681 atgctaccta aaaatagaat caatatttat atatctaaat tattaaatat attatttcta
     1741 gtgtatagct atacagtagt acttacaata tctttgttta ttgcaagtaa gttgttgcct
     1801 agacatatgc ttggtaatgt cacaaactat ggttttgtac aagaaacaat atatgagtta
     1861 aaaatgttac ttccatatag ctttgaaata ctatttgtag agtatatatt tttattgata
     1921 ggatttgtat ctgtagtatt tacatcaatt cttataaata aatctattta taaagtaagc
     1981 actttaataa gttttctatt tttagttata gaaattttag tgtttataat ttctatagaa
     2041 tttattatac cctacgaata cagtgatgta tttactgttc tatattcaag tataaacata
     2101 tttgtatgta gtcttatatc taataaactt ttgaagcaaa agattgactt ttaggaggga
     2161 tattatgaaa gcaaaaaata taggaaaata tacaatatgt tttacaatag tatgtttttt
     2221 aatgattact atgaccttaa tattgagtta ttttaataag gataaactat ctgtaaaaac
     2281 tttaaaaggc aatgtaaaag atatagggag agtaagtgta gtatatacac ctaatataag
     2341 caaattttct tatgaggagg taattataag taaaaatgga gtaagaaata atacaaaccc
     2401 aaaatcaaga attagaaatg attatttaaa tattgacaaa aaatacagtg actttgttaa
     2461 tgataaagga aaagaattta tatataaaga tgatttgaat ataggagaag cgtatacctc
     2521 tgttaagtct gatttagtac aaggaatgaa tgctttaaat gattgtataa aggtttcaaa
     2581 taaaaattta aaaagtgggg tagaggaaaa ttttgatatt aatttaaata agtctgattt
     2641 taaagaaaat agttggaaaa ccttatttaa caaaatatat aaaggtaatt tatatatggt
     2701 aattagtgaa actaatattt caggttctaa agtcaataat atttgtatat taaagataga
     2761 tataaaaaat aagtcttata aattattaaa tagatttaat ttaggattac agaatggaga
     2821 acaacaaaat atcaatgaaa ttaaattttc tattggaagt aaattatact tagaaatagt
     2881 aaataataaa agtaaaaatg gatatattga aggacatgat agttactcta actttattat
     2941 atatgatata gaagataaca cttttaatat aagtaataaa tttatggata atctaaacaa
     3001 agaaaagaat gcaaatccaa aggagattgg cttattaagt aaaactaagc tagattatat
     3061 ggtaaaaaat aataaattga atcttatagt tacggatatg agtaattcca taactaatct
     3121 tgtttatgat attaattcta atgagattaa attagaagat tatgagatac ttgatttaaa
     3181 agtagattat aatgaagcat catctggagt taggaaagtt aagctactgg atgataagat
     3241 atattctatt caggtattaa gaacagtata tcaggagaat taaaaggatt taaagggatt
     3301 gttgatgtag tcggaactaa gccagtaaaa tttcaagtat ttgatatgaa acttaaaaag
     3361 aatatttatg aagctgagct aataaatgga gatataagtt tacaagaaaa gttatttttt
     3421 gttgcaaatt aagtgttaga attaatttta gaactttaaa cttttatatg tatcacagat
     3481 aagtataagt aagggagtgt ctcaaaataa actttttagc tcatgagaca ctttttttat
     3541 atgaaatcaa atatattttc attgtttaga tttattttga taatgaaaaa aagattatct
     3601 ttattttaag aaagacattt atttataatt atatacaaaa atatttttta atgtttttgt
     3661 gtataattat aacaatttaa tttatattaa tgcacttaaa tttgaaatag aatgcttata
     3721 gtggtaaaat taattattag aatattagaa ataataaata aataatacat tgtacttaaa
     3781 agggggagaa atttaagatg aacaaaaaat atattaaagc agggaattta attgatggaa
     3841 ccggaaatag tattatgcaa aataagggta taattattga tggagatact atagttgaaa
     3901 ttgaagatat aagagatgat ttaaatggat atgatgtgta cgattactta gataaaactg
     3961 ttatgccagg cattatgaat tgtcatgttc acttgacaat ggagccagtt ggaaatccaa
     4021 agatatatta tgataatgta tcagatgtag agcttgtagt aaatacgata aaacaacttg
     4081 atgcttattt ggattcagga gtaacatata ttagaagctt aggttgtccc aaatatgtgg
     4141 atgtacaact taaaaatctt atagacaaag ggtctataga tggtcctggt atagttactt
     4201 caggtccagt tatatgtatg acaggagggc atggatatta ttttggtatt gaaagtgatg
     4261 gagtggatga atgtagaaaa tcagccagaa cagttttaaa aaatggagta gattgtgtaa
     4321 agataatggc aactggaggt gtaacaactg atggagttga gccagggtca ccacaattaa
     4381 ctttggagga aatgaaggct gctgtagatg aagctataaa agcaggtaaa ataactgcca
     4441 cacatgcaca aggaagaact ggtataaaaa atgcagtgct tgcaggaatt acttccatag
     4501 aacatggtgt ttatttagat gatgaaataa ttgatttgat gcttgaaaga ggaacttact
     4561 tagttccaac tgtggctgca ccatatttta ttctaaatgc aggaaaagaa agtggaataa
     4621 ctgaagctac tcttagaaag tgtgagattg ttgccattcc acataaagag tcattcctaa
     4681 aagcatataa aaaaggaatt aaaattgcag ttggttctga tgcaggaact tcttataatg
     4741 aacatggaaa aacatattat gaaatgaaac taatgtcaga ttatggtatg gataatatgg
     4801 atgtaatagt tgctgctaca aaaacagcat ctgagctttt aaaaattgat aaaaattatg
     4861 gaactttaga aaaaggaaag aaagcagatg ttatagttgt aaatggaaat ccagtagaga
     4921 atatagacgt acttgcagat gtgttagcag tatttaaact tggtaaaaaa gttagataaa
     4981 tagtataaaa ataaaaaaga gtgtctaaag aaatttttaa ataattatag acactctttt
     5041 ttattagtaa taattaaatc ttttatactt tagaaaatag attctatttt atatttagat
     5101 ataactcttt aaaatcattc ttatgcaata taatctatag aatctacaca tacaaagtat
     5161 attcttgaat cattagaatt tccaagtact ataacatcat ttttcttacc tagaacttca
     5221 acaccagtta gaaccaaact accagctact acagtaactt gattatttat atttgacata
     5281 taaagattat ctaggatacc tttattacaa cagcaatcat cacaatcgca gttacaatta
     5341 cattctttgt aatgtttttt atcaagatag taacctaaag atgctatgaa atcttcataa
     5401 tctgcaggtg taatatcaaa tgcaacagct ttaagattac acaatgatgc taaatctacg
     5461 tttgctaatg gagtagatgc agcaagtata aaatcaatta tagcagcaag tattgcatct
     5521 aatatagccc caaggtctat cgtaggagga ataactgcat caataagtgc aattaattca
     5581 gctactcctg gtatagttcc tatagtatta attaatccct caagagttaa agggactgga
     5641 ataggataag atactctacc agctatatct attaaatcac agttacaagc agaaaatctt
     5701 tcaaaagtac catcaagtcc agataaatta tcttttggag gagctgaaag atctatacct
     5761 accaaattag cacctactat aaagaaatct gagataaaag caaattgatt aaagtttaca
     5821 aaaggtctta gagcatcata tcttagaagt tctaaggcct ctctcatact ttttttacaa
     5881 caacattgtt ttattttagg ttctttatat ttatttggat aattatagta tctatcagct
     5941 acttgataat tttcatagta tctatcattt gtatttggat aatcttcctc atattcttgt
     6001 gtaaatctaa agtcctctct acatttatta ttttccacta aatacactcc ttttatattt
     6061 taatttaaat gta
//
LOCUS       DAEORZ010000191         5971 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEORZ010000191 DAEORZ010000000
VERSION     DAEORZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.195"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            197..1009
                     /gene="modA"
                     /locus_tag="KRH39_003481"
     CDS             197..1009
                     /gene="modA"
                     /locus_tag="KRH39_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8513353.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            1019..1810
                     /locus_tag="KRH39_003482"
     CDS             1019..1810
                     /locus_tag="KRH39_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513354.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFLSLTCLMLFEIFFNREVDKN"
     gene            1812..2507
                     /locus_tag="KRH39_003483"
     CDS             1812..2507
                     /locus_tag="KRH39_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8513355.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDIYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2494..3225
                     /locus_tag="KRH39_003484"
     CDS             2494..3225
                     /locus_tag="KRH39_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513356.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEICAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            3539..4339
                     /locus_tag="KRH39_003485"
     CDS             3539..4339
                     /locus_tag="KRH39_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG8513357.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFLVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            4879..5595
                     /locus_tag="KRH39_003486"
     CDS             4879..5595
                     /locus_tag="KRH39_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8513358.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         2327 a          724 c          844 g         2076 t
ORIGIN      
        1 aatactttaa agtattaata aaaattaaaa ataaaataac tacctatgga aatgacatta
       61 taaatagatt ttttcttaac tatacataac aaaacataac aaaacattat aaaacataac
      121 aaaaacttag acatcgtttt cttagtatga tatactaaaa aagaatttac actaatttaa
      181 taagggggaa tatcttatgt ttaaaaaact ttcagtacta gctttagcta tgattatttc
      241 attaagtatt actgcatgta attctaaaga aaacaaagct aatgatgaat ctaatactaa
      301 agtgacaaca gctgatgatt caaaagaaaa agagactctt tttgtctatt caggggcagg
      361 tttaaagaaa ccaatggatg aaatagcaaa aaaatttgag aaagaaaata atgtcaaagt
      421 tgaatatagc tatgcaggtt ctgcacagct aatagctcaa attgagacaa gtcacaaagg
      481 agacgtattt attgttggtt ctgaaccaat ttattcaaag gcctttgaaa aagaacttgt
      541 aggtgaacat aaaacggttg cacatcatac acctgcaata gttgtaccta aaggaaatcc
      601 agctaatatt caaaaattgg aagacttaaa taaagatgga gttaaactta ttttgggaga
      661 caaagagtct aatgctatag gtaagactac acaaaaaata cttgaaaaaa ataatttaaa
      721 atctataaat gataatgttg tctctacagc tgcaactgta aatgaaatga ttgtacagtt
      781 aacatcaagt aaagctgatg ctacaatagc cacaaaagat tcggtctttg gtaatgaaga
      841 tgtagaagtt atagaaattg cccaagataa aaatatagac caacttatat cagcaggaat
      901 cgtaaactat agtgataaaa aagatttagc taataaattt ataaattata tagagtcaga
      961 cgaagctaaa cagatattta aaaaatatgg ttttgaacct gttaagtagt gattaaactt
     1021 gaaaaataaa ttttttacta ctctacttac tggatttttt gctatagtct ttttgtttat
     1081 tgtatcacca cttcttatgt taatcattaa agggataagt tatgttccta tatgcttaaa
     1141 atcagtggaa gtacagtatg caataacact tagtattaaa acatctttaa tatctactat
     1201 agtatgcctt ttattagcta ttccagtagc atatttttta catattacta aattaccatt
     1261 taaaaaactg attatacaga taataaatct ccctatgtct cttccacact tagtatcagg
     1321 aattgctctt ttacttttgt ttggaagaat ggggattgga gattccattt ataagatttt
     1381 taagcttgat tttatattta caaagcaagg tatagtttta gcacaagtat ttgtaaatct
     1441 tccacttact attaaaatac ttcacacttc actaaatgaa agtaatgaaa agatgatatt
     1501 tgttgcacgt acccttggat gtaatagttg ggaagcattt agatttataa ttcttccaaa
     1561 tctaaaaact ggaatcatat ctgcaactgt aatgacttgg tcaagagcac ttggtgaatt
     1621 tggtgcagta gctatgatag caggttctac acgtatgaaa actgaaatta ttcctacatc
     1681 tatatattta aatatgtcta ctggtgatat agacattgct atcggaatag ctgtaatttt
     1741 gatatttctc tcacttacat gcttgatgct ttttgagata ttttttaata gagaggttga
     1801 taagaattaa tatgttaaaa ataaaaaatt tatctcttga tttacctaat tttaaattac
     1861 aagaaaatag tttaaatatt aataatcatg aatactttgt tttattagga gctagtgggt
     1921 caggtaaaac tctattccta gaaacattag caggaagata tccatctgct aaaggaaatg
     1981 tatcatacaa agaaaattta atatcaaact tacctccaga aaatagaaat ataggatttg
     2041 tatatcaaaa ttttgaatta tttccaaata tgaatgttga agataatata aggtttccac
     2101 ttaaattaag aaaaatacct atagatgaac agattcttaa ggctaaccac ttgtctaaat
     2161 taatgcagat agaagatata aaaaataggt atcctaaaaa tttaagtgga ggcgaaaagc
     2221 aacgagttgc ttttgcacgt gccttaatag ctgaacctga tatattactt ttagatgaac
     2281 ctatgagttc attggattat attacaaaaa aacatataag caaaattttg aaggatatct
     2341 acataaaata caaaccaaca gtgattcatg ttactcatga tatttctgaa gcaatgtttt
     2401 ttgctcataa gataggaata atgaacaatg gaaaaataaa tcatattttt gatttaaatg
     2461 aagaaataaa aaataaagga gaatcttttt tctatgaata tatttgatat tttgagagat
     2521 tatctattag tacaagtcga taaatataat ttaaatatgg atatgataag tattgtgtcc
     2581 aaatctctat cttcaaaaga agcaattgga aatactaagc gtaaagattt cccaattata
     2641 gttggtaaag aaataatgct tgaagctgac tttaaaggag ctaaaggaca agcatttact
     2701 tctactccat ctacatttga aggttcttta aaagacatat tatcattaga tttacatgac
     2761 aatcctcatg atagaagtct ttttatagca tcattaaatg cagttatgaa atacttaggc
     2821 aagacggata gaaccattca ttgtaaaaat aacgagccag agatatgtgc aaaaaaattc
     2881 cctgagttta taaaaatgga atttggtaat ccaaaagtag caattatagg atatcaacca
     2941 gccataattg ataatattaa ggactttttc gaaacaagag tattagattt aaatcctgaa
     3001 tttgtggata ctatccaata taatgttaag attgaagatg gtatacgaga ttatgaagat
     3061 gtaatatctt gggctgattt agtgatttgc acaggaagta cattatgtaa taactcaatt
     3121 ataaattttc tatcacttaa caaaccagtt tactattatg gaactacgat agctggtgca
     3181 tcaaatattt taggtttaaa aagactctgt ttttgtagta aataagcaat catatcatca
     3241 aggagtacac aaatatatat gtgtactctt taaatttaaa aagttattac ttttattcta
     3301 agtactaaag taaaattcta ttaaaatgaa tcccaaataa atgtacaatc tctttaaatc
     3361 tcaattccta tcaatcatta atattttgat tagtcttata cataaatgtt ttctttattt
     3421 taaactaata aaaaaatttt attccattaa tctactctat atttttcagc atttaagaaa
     3481 taatttttag tactttgttt gataatataa ctaagacaac ttttcaggag atgattaaat
     3541 gacatttaag aataaaaaat ttactataaa taaatataaa attctatcta ttattctagt
     3601 tttatgtttg attttaaccg cttttagtag ctatgtgaat tatagtcata aaaaatttaa
     3661 agattatgtt gtcttctatg ttcagcatca agatgatgaa gtgttgtggg ctgggagtgc
     3721 tattgttaat gctataaaag aaagaggaaa aaatcatgta tttctggttt tagtatctac
     3781 tggatgtggt atatccgtct ttgataaata taaaaaatat gaaaacctaa caaatataca
     3841 aaaatcagaa tatagaaata gagaattttt agcttctctt aatagccttg gtgtaataag
     3901 ggaaaatata atattattac cagaaacaaa tacaaatggt aatactgact tcaattacat
     3961 ggaaaaaatt gctcttacat ttgaagaaaa atttaaaaat gtaactcata tagcacatac
     4021 ttataagtta gatgaccatt tacaacattt aaaaaatggt tctgttatcc aaaatttata
     4081 taatgctggt aaaataaaag atgctaaata ttttgtaaaa cctaaatttg ctgataaaat
     4141 aacttttaat aataagatag tttataaagc tcattctact gaagactatg aaaaggttaa
     4201 aaatgcctgt caacaataca aaattgttga tgaattagaa tatagagaag gtattggcta
     4261 taagtctgac cataaatcat ttgataagtt attaaataat aaagatgttc ctgctatact
     4321 tcatacacct aatttataag aaatatttat tcttttaatt taagttataa aaaggaaatt
     4381 atttgagatt ttgtgatata gcactttttg tgctatacta ctttttctca tacaactatt
     4441 acccatatct aatttcatca tctcttaaaa tatataaaga tatcatcatt aaaacctata
     4501 agccttatta aatgctttat acatacctac attaaactac ctgtttatat taaactacat
     4561 taaaataaat atttgttata aattcttaat aattatgaaa ctcatttgtt atttatattt
     4621 gttaatatat gtttaaacct aaaaaattaa taaaccaaaa taacataagg agtgtgatag
     4681 attaccctag atattttcat aaacttttac cttcagatta atatgggcgt ctatactttt
     4741 aaatttacta tatgcattga caatatatta aaagtactag tttttatttc ataatgaatg
     4801 ggcttactgt tttctaatac ctctttttgg tataaaataa aaattggtta aaaataaaat
     4861 ttcaaggggg ttaataagtt gaatcaaaat atattggttg tagatgatga tagagagata
     4921 gttgatgcta tagagttcta tttgagacct gataacttta atgttttaaa ggcatatgat
     4981 ggacttgaag cattggacat actaatcgaa aatgatatta agttaataat aatggatgta
     5041 atgatgccca atttagatgg gcttaaaact acacttaaaa taagagaaaa aaggaatata
     5101 ccaataattc tcttatctgc aaaatcagaa gatatggaca aaattatagg tcttaatatg
     5161 ggtgctgatg actatataac taaaccattt aatccactag aacttactgc tcgagtaaaa
     5221 tctcaactta gaaggtatat aaatttaggt gattttagta atacaaataa ctcaaatgat
     5281 actaattata ttctaaaaag tggtggccta gagctaaata cagatacaaa aatagtttct
     5341 ttagatggtg aggaaattaa aatgacacca cttgaatata aaatacttac acttcttatg
     5401 tcacgaaaag gtaaaatttt ctcttctcat gagatttatg aaaatgtctg gaatgaggat
     5461 gcttatagtt gtgagagaac tattgctgtt catattagaa gaattagaga aaagattgaa
     5521 attaatccaa aagaaccaaa atatttaaag gtggtgtggg gcattggtta caaaattgaa
     5581 aaacttaatt attaatgatt ctaaagcaaa atttatttta ttattattac tgtttctatt
     5641 agtttctata ttatttgaag cagtttatga tttgaataat aacttttctt ataatattaa
     5701 ttgttattat aaagagtttg ctctccctta tttttatttt ttcggtactc ctgtacattt
     5761 gtcaaaagta gaattagagt ttgtctgcat aggtattata ttatcaagtt ttttattgac
     5821 tatactatgt tttacattgt ataaaaaaaa taaacagact aaagtcaaat tcttagagaa
     5881 tgttataagc cttttttcta aggtaaaaat agaacttaca cttatattaa ctttgatact
     5941 attaacctta aggtttcatg catttacaag t
//
LOCUS       DAEORZ010000192         5958 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEORZ010000192 DAEORZ010000000
VERSION     DAEORZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.196"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            16..684
                     /locus_tag="KRH39_003487"
     CDS             16..684
                     /locus_tag="KRH39_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBG8513359.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFKVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
     gene            complement(775..2226)
                     /locus_tag="KRH39_003488"
     CDS             complement(775..2226)
                     /locus_tag="KRH39_003488"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBG8513360.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENDNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNILNLNLNSDEIGSFVEGILLGNYKFDKYKTKSKDFERKEVDTMTIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAIETVKLGEEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNDDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGSTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(2242..2682)
                     /locus_tag="KRH39_003489"
     CDS             complement(2242..2682)
                     /locus_tag="KRH39_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8513361.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            complement(2930..3358)
                     /gene="ytfJ"
                     /locus_tag="KRH39_003490"
     CDS             complement(2930..3358)
                     /gene="ytfJ"
                     /locus_tag="KRH39_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBG8513362.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            complement(3312..3941)
                     /locus_tag="KRH39_003491"
     CDS             complement(3312..3941)
                     /locus_tag="KRH39_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBG8513363.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTENIGLTSSVYVLINTLYGNLFNIIDAEKMYLNVNPDFTKDFVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            complement(4103..4642)
                     /gene="scpB"
                     /locus_tag="KRH39_003492"
     CDS             complement(4103..4642)
                     /gene="scpB"
                     /locus_tag="KRH39_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBG8513364.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            complement(4644..5363)
                     /locus_tag="KRH39_003493"
     CDS             complement(4644..5363)
                     /locus_tag="KRH39_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBG8513365.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(5393..5938)
                     /locus_tag="KRH39_003494"
     CDS             complement(5393..5938)
                     /locus_tag="KRH39_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBG8513366.1"
                     /translation="MAISIHEFGHAYSAHLLGDDTAKAYGRMTLNPVRHVDPIGLLAM
                     FILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAILCVIINKYVHMYAINTI
                     AEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYVYKFESMSYIILLIALFTG
                     IYRIILTPIYAVLLKIVYLFV"
BASE COUNT         1902 a         1000 c          543 g         2513 t
ORIGIN      
        1 aggagagaat gttatatgtt taaaattttt cttttttcta gtgaacaatt tgtaagtctc
       61 tttatatttg gattattttt atactattgt ccaaaactaa ccaaaaatat tttaccttat
      121 agctatacag ttgagaagat aatttgtaca ttacttgtaa taattatggc tctagagcaa
      181 ttattactta tttcttcagg taattatagt actctaaact ctttaccaat tggaatcaat
      241 tatatttgta tatatctgtg tatagccata ctaattttta agcagtatca tctttttaat
      301 atattttttt cttggagttt agtttgttca gttggagaac tcattttttc taaaaacttg
      361 ggctatgaat ttcctagctt aatatatttt atatttatct tctctaagtg cttgataatt
      421 tatgcagata tatatatggt tgatgttaga aaatttaaag ttaacagata tgcacttaga
      481 gataacttag ctatatgttt tatttatttt tcattcatat ttttactaaa cacatttacc
      541 aactctaggt actattatgg ttttttaagt catagcacaa ctgctatttt tacatttatt
      601 tttgttacta gtattatgta catacctgct cttttattta atagagatac ttttatactt
      661 gaaaaaaaga aaaagagtaa ataatttact taaataaaaa ataaagccac atagataatc
      721 ttaaactaca aaaatatcca cgtggcttta taaattatat tttataattg ttttttagat
      781 ttctgccaaa tagtacaatg ttctaacggg ttctcctgta gccccctttg ctttatatcc
      841 aattgatttt gatgatgtac ttgtccctgc tatatccatg tgtatccatg gagtactacc
      901 aacaaattca ccaataaaag ctcctgctgt tatacatcca gcatttttac caccagtgtt
      961 tttaaggtcc gcttcttctc cttttatcat atctttaaat tcatcataaa ttggcatctt
     1021 ccatactctt tcccctgata aagtggccgc tttttctaat tcacaataaa aatcatcatc
     1081 atttgttact ataggagttg caacattgcc caacaatgtt aaagcagcac cagttaatgt
     1141 agctacatca accacctttg ttactttttc attgtttata atgtagtata ttgcatcagc
     1201 taatgtaagt ctaccttccg catccgtatt taaaacttca atagttttct ttgccataga
     1261 actaattata tctccaggtt tgtatgaacc tcctgataaa gcattctcac atgcagccac
     1321 tactgctata acatttttct ttaatttact ttgagatata gcacacatag cacctataac
     1381 actcgcagcg ccagccatgt ctgtcttcat atctagcata gaagcatttg attttaaaga
     1441 atatccacca gtatcataag tcaatccttt acctactaga cctaatatat ctgtactttc
     1501 tttatctcca aagtatctca ttactatgaa tcttggttta ttaattgaac ctcttgaaac
     1561 agcgaaaaaa gcatccattc ccaatgcctt tattttttcc tcttcataaa catcaacatt
     1621 aaacccaaac tcttcaccaa gtttaactgt ttctattgct aatgtttttg gatatattac
     1681 gtttgatggt tcattaacta gatttctagc tattatagtt gaattagcta atattttagc
     1741 cttttctatt tgacactcaa cttctttttc agtaaatata gtcatagtat caacttcttt
     1801 tctttcaaaa tccttacttt ttgtcttata cttatcaaac ttataatttc caagtaatat
     1861 cccttctaca aaagaaccta tttcatcact atttaaatta agattcagta tattaacatc
     1921 actactctta tatgcttcat ttttcaatat attttttaat tttttacaca aatttccacc
     1981 tatgattcta attttttctc tattcaataa ctctctctta ccaaaaccaa caagacaaac
     2041 atttaccaaa ttgtcatttt caaccaatgt tacagtatct atactatttt ctttagaatt
     2101 aaaatttctg cttatcatat ttattttatc aaatgtcgta gaaacatctt ctccctcaaa
     2161 tagagctact actgttatag cattttctaa actttttaat atttctttag aatactttat
     2221 attcataata aattctcctt tttaaaataa ttgttctatt atatttttag actcttccag
     2281 agaagtaaat aatttatctt tattatctat atctaagcaa tccattttat cacttattat
     2341 agatacaaac tttagtattt cattatccaa tatcttttct ccatttttag ttattccata
     2401 aaaagtattt cgtctatctc tactatcttc ttccctatat acatactctt gctcaaccaa
     2461 tttattaagt agtatacaca aactagaagt agatacatgt aaatctctac ttagattttt
     2521 aagctctatt ttatcatgtt ttcttatttt tactaaggca aagaactgtc tttctgtaag
     2581 aaccttatct tttttatatc tttcgttatt aataatcttt attaaattat gaaaattatt
     2641 catactttct aacaacaaaa tagataattc tttatcgctc ataacattca cctcaaaact
     2701 aatgatttat aactccctct tatatctcaa atgcgtttat atatctataa tataatactt
     2761 cttttctttt tataattata ctatatatca actttattat ctatttaaat tgttttaaac
     2821 taaaatctta ttagatataa atgtttaaag tacacaacaa aatagtggga tttatactaa
     2881 ccccactact ttatttaaaa ctatatacat atttaaaaat attttattct tacatattgt
     2941 ttttatcttg attcttatta ctttgtgata tattttgaat tttctccaat actctaggag
     3001 ttatagacaa tatattatcc actaaattga tattactgtc taaactcatt attctagtct
     3061 ctccactttc aactactaca aatgctactg gttgtacaga tatagcccct gcgctacctc
     3121 cagcaaaatt cgtatctgat ttttcatttt ttaattcaat ttccctatct ttatcatcat
     3181 atccttttga gtattctcct cctcctatac caaagccaat tgtaaccttt gatataggta
     3241 caaccactgt agtatctgtt tttatagggt ctcctataat agtatttgca tctatagaac
     3301 ctttaattgt ttcaagagtt gtttccataa gactctgtat cgaacctgtt gctctcatta
     3361 ctatcacctt ccttatttcc tttattctta ttcataattt tattaatcat tattataata
     3421 ttaaataaag cttttattct tggccttata tgtatcctta tatttcctaa aacaaaatct
     3481 tttgtaaaat ctggatttac atttaaatac attttttctg catcaattat attaaataaa
     3541 tttccataca aagtatttat caatacatat acagaagatg ttaagccaat attttcagtt
     3601 ccaaaattaa tatttgaata aagttcatgt atttttatct tttttaataa tctataaatg
     3661 ctcaaaaggt catcagctaa taatatactt gaatctatat tattcttcat cccctctttt
     3721 ttatcattat ttttactatt ttcagctgga taaagttgtc tatttatatt tattaaatta
     3781 aacatgtatt ttatattaag ctttaaacaa atatctttgt tttttatatc agctattatt
     3841 aagatgttta tatgagatat taaaatcaac atagttacac ttataataaa aattacagct
     3901 attattaaat acttaaaatt aaataattca ttaagctcca tgtttatatt atcatcaaaa
     3961 aattactatt tatacttaat tatccatatt tattttaaga gaattataat tttatattta
     4021 tacaaaaaaa cacaacaact tagagcattt taaatctttt aaaatttaaa attacatact
     4081 aaattattgt gattttatat ttttattcat ttgttgccac ttcttgataa ttttctatag
     4141 gtggcaattc ttcaagactt tctatattca acaactttaa aaattcatct gttgttttat
     4201 aaattattgg tttccctatc ttattcaatc taccagcttc tcttataagt tcattttcaa
     4261 acaaagtttg taatacctta tcacatttta ctcctcttat atcctctatt tctacctttg
     4321 ttattggttg tttgtaggca attatagtaa gagtttctaa tgtagcttgg ctcaaacttt
     4381 tctttttctt tggttcaata atcttcttta tatattctgc atagtcttta tttgtacaca
     4441 tttggtactt attctctaac ttgattattt gaatacctct attttgttct ctgtactcct
     4501 caataagtaa atttaacata atctctattt cttttgatga aagttcttta tttattatat
     4561 agtttaattc ttttatactt atcggttcac catatgcaaa cataacagat tctataatat
     4621 actttatatc ttctcttttc attttaacac tccgaacttt ttcttattag aatatcatca
     4681 aaaaatatgt cttgtactac tactatttct ttttctttta taagctctaa tatagataaa
     4741 aaagtagcta ttatttcatc attttcatat gattttataa tatttgtaaa agtaacttca
     4801 atcttatctt ttataatatc tcttatatat aatatttttt cttctacaga tattattttc
     4861 ttccttacta ttttatctag tttctcatca ttagcatttt ctatctgtga tgttttaact
     4921 ttaaatatgt atggaagtat attttttatt gcttctattg atatatcttc taaatccaca
     4981 ttgtcatcaa taattatctc ttctttattt ctataaaatc tctcattaac ataagttata
     5041 ttttctttta tatcttgtga tgctacctta aactttctat attcttctaa cttttccata
     5101 agttctattc ttgggtcttc ttcttcatct ttttgcttat aaagcagata tttagacttt
     5161 atctctaaaa gttttgatgc catagttata aattcactag ttatctctaa atccatttta
     5221 tctagcattt tcaaataatt tagatactgc ttagttatat ctattataga tatatccttt
     5281 atgtctattt tatgctttgt tattaaatca taaagtaaat ctaatggtcc ctcatagact
     5341 tgtaattgta tattatactt catatcaatc cctctttaat ctgtttatta tattaaacaa
     5401 ataaatatac tatttttagt aatactgcat atattggtgt taatattatt ctatatatac
     5461 cagtaaataa agcaattaat aaaattatat aactcatact ttcaaattta tacacatatt
     5521 cattccattt atatggtaca aatgtagata taactcccca tccatcaagt ggaggtatag
     5581 gaagtaagtt aaaggctgca aatccaatac tatataagaa cacatattca gctattgtat
     5641 ttatagcata catatgcaca tacttattta taattacaca cagtatagca gtcaaaacat
     5701 tacaaaaaac gcctgcaagt gaaactatca ggtttcctat cttatagttt ttaaagttat
     5761 ttggatttac tggtactggc tttgcccacc ctatcttaag gataaacata gccaaaagac
     5821 cgattgggtc aacatgtctt acaggattta atgtcattct accataagct ttagctgtat
     5881 catctcccaa taagtgagct gaataagcat ggccaaactc atgtattgat attgccaact
     5941 aaacttgcta ctatttct
//
LOCUS       DAEORZ010000193         5879 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEORZ010000193 DAEORZ010000000
VERSION     DAEORZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.197"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            333..848
                     /locus_tag="KRH39_003495"
     CDS             333..848
                     /locus_tag="KRH39_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBG8513367.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      1340..1444
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1654..4128
                     /gene="gcvPA"
                     /locus_tag="KRH39_003496"
     CDS             1654..4128
                     /gene="gcvPA"
                     /locus_tag="KRH39_003496"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBG8513368.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            4128..5585
                     /gene="gcvPB"
                     /locus_tag="KRH39_003497"
     CDS             4128..5585
                     /gene="gcvPB"
                     /locus_tag="KRH39_003497"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBG8513369.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRDDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         2326 a          622 c         1055 g         1876 t
ORIGIN      
        1 ttacagtact taattgtact gaaaaaaact ggggttgctg gctttcggag tgggctatcc
       61 cgtttttatt tttttgtata tataatagat tggcacttta gtataaggtg ctttttttat
      121 aggcaaaaac agtacattta tttctgtttt attatcattc atctattaga atatacttta
      181 gttaagaatg tgttaataag cagtgttcac attcttgata aaatgtggaa taatatgtat
      241 ttttactaag acggaaatga aataattaaa ccattaaata atattataag atgatgaata
      301 agtagataaa tcaaattttg taggaggata ttatggaaaa atatatatgg ttgtttccat
      361 tactttttat ttttcatgat atggaagaga taataggttt tggaatatgg cttaaaaaaa
      421 ataaatcaat gcttgataag aagtatccat ttatcagtaa agtttatgaa aattatagta
      481 ctgaaggcat ggcatttgct gtgtttgaag aatttatttt atgcattatt ttctgtatac
      541 ttacagttat aacagagaat cagtatgtat atctactatg gttaggtagt tttatagcat
      601 atactttaca tttggtaatt catatagggc agtcaatcat aataagaaaa tatatacctt
      661 cacttattac cagtatcata tgcttaccaa taagcatttg gtgtataagt aagagtattt
      721 atatagttga ttgtgaaatg agtacaacaa ttctgtacag tataattggc attataattg
      781 tagcattgaa tttaaagttt gcacagtcgc tgattggaaa atttacaaag tggatgtcaa
      841 acatataaat tttagtttat tggacagttc aaggaatcag aaattgaaat tttatagtat
      901 taatatttta tagtattaat ataatgtata aaattttata gcaatgtatt tcataagtat
      961 aaagtgacta gaattttaag aaatatacaa attctataaa aactaaataa tttcaagtgt
     1021 attttaaaaa tcttattttt cacaagttta aaattgtaaa cgatttcatc aagtctatga
     1081 atatactttt aacatgttat agtaaatctc aatataaatt tacatttggt tctttgtgtg
     1141 ttattttaat aataaattaa gtgaaaatgt tattttacat taatatataa tatctttgca
     1201 taagagtgaa taatataaaa gagaaataat tgataataaa aacttaacaa aaaaattaat
     1261 acataaaaaa aaataaatat aatgtgttga catttaattg aaatattcat acaataaaat
     1321 tgaaaataaa tatttccaaa tgagattagc gagagagtcc tttatacgaa aaggcgccga
     1381 agaagaaatt taagaaatgc caatttttag agaagctttc aggcaaaaat atcgctaatt
     1441 gatgggactc tggagagact taagttaaga tactataagt caccgaagaa gcaaaataat
     1501 attaaattat taaatctttc aggtatacaa acagggatat ataggctagg attattttcc
     1561 atgctgatat atcttttttt tatgaaaaag tacaggaaat aatatcaaag ttacttaaat
     1621 acagtaggtc taattttcta gggagggtta tatatgaatg aattgaaaag agtatctttg
     1681 tacaatatac acaaagagct tggagcaaaa cttgttgaat ttgctggttg ggaaatgcct
     1741 ttagaatatg agggtataaa taaagagcat gagaaggtca gaaaaagtgc aggtatattt
     1801 gatgtatctc atatgggaga ggtacagata aagggagcag agtcagaaaa atttatacaa
     1861 aacctagtta caaatgatat aagcacatta aagataaatg acattatata tacacctatg
     1921 tgttatgaaa atggtggagt agttgatgat ttattaatat ataaatttgg tgaagaagat
     1981 tatttattag taataaatgc aggtaatata gataaagatg tagcatggat aataaaacaa
     2041 agtgaaggat acaatgtaga tataaaaaat atatctagtg aagtgagtca attagcaatt
     2101 caaggaccta aagcagaaga aattcttcaa aaaatcacag atatagactt aaactcaata
     2161 aaattttata agtcaattcc atcaacaaaa gtatgtggtt gtccttgttt agtttcaaga
     2221 acaggatata ctggggaaga tgggtttgag atatactgta aaaataaata tgtagaaata
     2281 atttggaatg aagttctaaa ggttggagga gaagatatat gtccagctgg tctaggttgt
     2341 agggatactt tgagatttga agcagctcta cctctatatg gacatgaaat aaatgaacac
     2401 atatctccaa ttgaaggtgg tttatctatc tttgtgaaaa caaataaaga atcatttatt
     2461 ggcaaatcaa ttttatcaaa agaaaaagaa agtggagcta aaagaaagtt agtaggattt
     2521 gaaatgcaag gtaaaggaat gcctagaaat ggatatgata taaggattgg tgataaaaca
     2581 gttggatttg taacaacagg atgtgcatct ccaacgacag gaaaaatttt aggtatggga
     2641 attatagatt ctgaatatgc aaaagtagga aatgaaatag gtatagcaat cagaaaaaaa
     2701 gttgttcctg ctgtgatagt taagaaacct ttttataaaa aacaatataa aaaagataat
     2761 attatattaa acaaagaaaa taaattttca tatatacctg ctacaagtga agataaatca
     2821 aaaatgttaa aagtagttgg attaaattca gttgatgaat tattttcaga tataccagaa
     2881 gaagtaaaat taaaaagaga tttaaatttg gaaattggaa aatctgaatt agaggtaagc
     2941 aaaatagtaa aaagattgtc agaagaaaat ttaagtttag aagacttgac ttgttttctt
     3001 ggtgctggtg catatgacca ttatatacca tcaattataa aacatataac ttcaagatct
     3061 gaattttata cagcatatac tccatatcaa gctgaaataa gtcagggaac tttacaagta
     3121 gtttttgaat ttcaatctat gattgcagaa attacaggta tggaaattgc aaatgcatca
     3181 atgtatgatg gagcaactgc tgcaatagaa gcatgtataa tggcaatgaa tcagactaga
     3241 aaaagtaaaa tagtagtgtc aaaaactatt catcctgaaa ctctgtcagt attaagaact
     3301 tacttacaat ataaagattg tgagatagta gaaattgact tttgtaatga atatgggact
     3361 acagatatag aaaaattaaa agcttctgta gacaaagata cagcatgtgt tcttatacaa
     3421 actcctaatt tctttggaat tattgaagaa atggaagaaa tagaaaagat aactcatgaa
     3481 aataaggcta tgttaattat gagtgtagac ccaatatcat taggagtttt aaaaacacca
     3541 ggtgagatag gtgcagatat tgttgttgga gaagctcaat cacttggaaa ccctcttaat
     3601 tttggaggtc cttatgtagg atttttagct agcaaatcta aatatactag aaaaatgcca
     3661 ggaagaatag ttgggcaaag tttagatgta gaaggtaaaa ttgcatatgt attaacttta
     3721 caaactagag aacagcatgt aagaagagaa aaagcaactt ctaatatatg ttcaaatcaa
     3781 gctttaaatg ctcttgtagc ttcaatatat atggctacta tgggtaaaga aggatttaaa
     3841 gaggttggta tgcagtctat gaaaaaagca cattatactt acaataagct tgtacaaact
     3901 ggaaaatata agccaatatt taaaggtaag tttttcaaag aatttgctgt acaaggaaat
     3961 cttaatatag aaactataaa tgataaactc ttagaagaaa atatattagg tggttataat
     4021 ttagaataca attatccaga attaaaaaat tcaactttgc tttgtgtgac tgagaaaaga
     4081 agcaaagagg aaatagataa gttagttgga ataatggagg gattataatg aaagaataca
     4141 atagtttgtt gatagatata tctaaaaaag gtaggaaagc atactcactt ccaaagttag
     4201 atatagatga tataaaaata gaagatatga ttgaccaaga tatggcaaga caaagtgagt
     4261 tgaatttgcc agaagttggt gaattagaat tagttagaca ttatacatta ttatcaaata
     4321 aaaactttgg agttgataca ggtttttatc ctttgggttc ttgtactatg aaatataatc
     4381 ctaaaataaa tgaagatatg gcagcacttc caaactttac tggaatgcat ccataccaat
     4441 cttcggatac agctcaaggt tctctttctt tgatgtatga tttatcaaga agacttgcag
     4501 aaattacagg tatggatgaa gttacactac aaccttcagc aggttctcat ggtgagttta
     4561 cagggcttat gattataaaa gcatatcatg aaaatagaga tgaccataag agaaccaaag
     4621 tgataattcc agattctgca catggaacaa atccagcaag tgcagctatg gcaaactttg
     4681 atgtgattca aatagcatct gataaaaatg gagctgtaga cataaatgca ttaaaagagg
     4741 ttttgaatga tgaagtagca gctcttatgc ttactaatcc aagtactctt ggtttatttg
     4801 aaaaaaatat aaaagaaata gctactcttg tacatgaagc aggaggactt ttgtactatg
     4861 atggtgcaaa catgaacgct attatgggga taacaagacc tggagatatg gggtttgatg
     4921 tggttcatct taatcttcat aagacttttt caactcctca tggaggtgga ggtccaggag
     4981 ctggtcctgt tggagttaaa aaagaattag tacctttttt accaatacca gtaattgaaa
     5041 gaaaagaaga taagtatgta ctaaattatg atagagaaaa atctattggg aaaattaaaa
     5101 atttctatgg taattttgga gttcttgtaa gagcttacac atatatacta actatgggaa
     5161 gggatggact taaagaggct agtgaaatgg cagttttaaa tgctaattat ataaaggaaa
     5221 gtataaaaga tgattatatt ttgccaatag ataccttgtg taagcatgag tttgtattag
     5281 gtggattgcc aagaggagag gccaatataa aaactatcga tattgcaaaa agactactag
     5341 attatggata ccatcctcca acaatgtact ttcctcttat tataaatgag gcacttatga
     5401 tagaacctac agaaagtgag agtatagaga cattagatag ttttatagaa gctatgaaaa
     5461 gtgttgctaa agaggcaaag gaagaaccag aactattgaa aactgctcca cataatactt
     5521 tggtcaaaag agtagatgat gcaagggcag taaagaaacc tatactaaca tggtcacaaa
     5581 gataaatagt taaaaagtag agatttaaag tataaagaga attaaatttg taagaaaaag
     5641 ctatctaact taatctagtt tagatagctt ttttgtatat tagtatgtat ggtattttct
     5701 atataaagtt ttaaattgta ttagtgctta tatagaatat tgatatatta tgtatagata
     5761 ttaataagaa ttgttgaatt tatcttgcct taactttatt tactctttta tattaagaaa
     5821 aacttaacta aaatatagcg aaattcataa aaatttagga aatcttaaga attataata
//
LOCUS       DAEORZ010000194         5852 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEORZ010000194 DAEORZ010000000
VERSION     DAEORZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.198"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..391)
                     /locus_tag="KRH39_003498"
     CDS             complement(<1..391)
                     /locus_tag="KRH39_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513370.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTK"
     gene            complement(865..1749)
                     /locus_tag="KRH39_003499"
     CDS             complement(865..1749)
                     /locus_tag="KRH39_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8513371.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2040..2516)
                     /locus_tag="KRH39_003500"
     CDS             complement(2040..2516)
                     /locus_tag="KRH39_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBG8513372.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEDVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDENCGCGCNHHHEN"
     gene            2783..3958
                     /locus_tag="KRH39_003501"
     CDS             2783..3958
                     /locus_tag="KRH39_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBG8513373.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNEDNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4039..4842)
                     /gene="proC"
                     /locus_tag="KRH39_003502"
     CDS             complement(4039..4842)
                     /gene="proC"
                     /locus_tag="KRH39_003502"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG8513374.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAITDAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(4882..5466)
                     /locus_tag="KRH39_003503"
     CDS             complement(4882..5466)
                     /locus_tag="KRH39_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8513375.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         1955 a          995 c          651 g         2251 t
ORIGIN      
        1 ttttagtaaa ttttacggta agtaaaaacc tctctccttc aaatcctata gcaaatatac
       61 ttacaagttg ctttgagaaa ataactccta atatagctac aactatgcat ataccaacta
      121 ttatatttaa aacattgttt aaaaacttta gtgcttgctt ttcaccttgt ttactactta
      181 tatcttgata cattggaata aaagtagtaa ctatagcagt acctattgct gcaaatataa
      241 tatttggtat attcatagct gttaaataac tctctgtata taagcctgta ccataaattg
      301 atgataatac taattctcta cccattccaa gaatcttaga gattatagta actatcatca
      361 aataaaaagt tgctttagct accttgctca ttttatcact ccacttctaa catacctctt
      421 tttaatcaag taaaatttag ctcattagat ataattctat caactaattt gccttgctta
      481 gccctatcaa aatataatta acacctattt cttaatatat cacaaactac attattcttc
      541 atattttttt atttctccaa taagttttac atttttccta tataaattgt atagcttcaa
      601 ttatattcaa agaaatggag ttgctacccg ataactttaa aagtaaaaaa cagattttat
      661 tatcttttta aactatattc taacatattt tgtccatatt gagaaatttt actaaactat
      721 tatgtacttt attaaataaa taaaataaca gcacacctat aaaatacaag tgtgctgttc
      781 ataaacagat tttataaaaa aataattcct aagtagagat taacatgctt tctcatactc
      841 tcatatataa tgtgtctaaa aatattaata aaaatgcttt aattgtttta acttacttgg
      901 atgtcttaat tttcttattg ctttagcttc tatctgtctt attcgttctc tggtaactcc
      961 aaataccttt ccaatctgtt ctagtgtttt agggtcatca tcatcaattc caaatctcat
     1021 tcttaaaact tgctgttctt gctctccaag acctgtaagt agttcttcta tcttttcttt
     1081 taaattatgc tgttcaactt cgtgtattac aacatcttta aaatcagcat ctgatggtat
     1141 aaattcaact aaactactat cctcatcttc ttttaatgga gtatccaaag atacaacatt
     1201 tttatctctt cttaataatt ccaacacttt gtcaacttct aagccacatg catctgcaat
     1261 ctcatctata gttggctctc ttaataacac atttaaaagt tcttgtttct ttttctttac
     1321 aaaatttatt ctctgatgaa gatgtatagg tatcctaata gtattttcgc aatcgtcgat
     1381 atatcttgtt atactttgtt ttatccacca agttgcataa gtgctaaatt tgtatccttt
     1441 tttataatcg tacttttcta cagcttttat aagtcctata tttcctgctt gaattaaatc
     1501 caacatatca atactatctc ttttatatct ctttgctatg ctcactacca acctataatt
     1561 tgaatttata aatttttctt ttgctactga agatgagttg attatttcct tagccagctc
     1621 tacctcttct cctgctgaca acattttgta ttcctctatt tcctttaaat acattttcat
     1681 cgcatttgat acatttgatg aacccacaca aatataattt tcataattat tttctttttt
     1741 agtatccata atcaaacccc ttctattcat agaatttgct aaaacaaaaa taaataattt
     1801 aaacctacat aatctacaat tacataatat cccattcttt aaatgaattg ttttgtatat
     1861 tatgtataca aatttataaa aactatacct ataatattat tctacttcta acagcaatat
     1921 cctccttata tatataattt attttattat atgttgttat attcttagtt ttttgaatga
     1981 tatatgtcta aaaaaagact gtttactaaa cagtcttttt atatatatat gctcaatatt
     2041 taattttcat gatgatgatt acatccacat ccacaatttt catcatggtg atgatgttct
     2101 tctaaatctt ttgcctgttg ttccatttca tgaaactctt ttaccattat atcataagtt
     2161 ggaagagctg cctcagtatc gtattcttcc cctctccaat cagcgtacat catagcatcc
     2221 ccattagtta aaaccaatct acctgacata gttatattat ccacgtcttc taaaaattct
     2281 atttgttttt gtaaaacttc tccttctaac tccacatctt catctaaaca ttgcactatc
     2341 attacaggtg cataataatc ttcttcatct ttaacattta caacaatatc taaaattcct
     2401 tctgcacctt cttcaaattc tgccaactca acatttgtat caatcatatc ttgaggtact
     2461 aataaatctt ctattaaata ttttattact ctatctttta ctaattcttt tgacatttat
     2521 ttttctcctt ccgattctat aacattatca taatcatttc tatattatag tatactacat
     2581 taattgtttt ttcaatctct tacaaaaatt atttttttat tatcttcctt atattccctt
     2641 ttttattaca aagttgtaat cgcattgatt taaaaaatag tttatattta gtataataga
     2701 aatgtagcac atatttttat tcaatatatt aataaaaaaa tatattgaaa atgtaatgta
     2761 tgttaaatct aggaggaata ttatgaagtt taaaaaacaa attagttcta ttgttgtgtc
     2821 tactatgctt gtacttggga gtatgaacct ttcttatgca gacggtacaa atgtagttac
     2881 acttggagct aacttatcag agtctcaaaa gcagcaaatg cttaattact ttggagtaaa
     2941 aaaagaccaa gtcttagttt tagatgtaac aaatgcagaa gaaagacagt atttacaagg
     3001 tgttgcaaca gagggtcaac ttggaaaagt tactatctct tgctcatatg ttgaacctac
     3061 taaaaaagga aatggaatta atgttaaaac agcaaactta acttgggtaa ctagttcaat
     3121 gattgcttca acccttacaa cagctggtct tgaagatgca aatgttattg ctgctgctcc
     3181 atatcctgta agtggtactg gagcattgac aggaatcatg aaagcatttg aggatgcttc
     3241 aggaaagaaa cttgatgaaa ctaagaaaga aattgcttct gaagaattgg tagttacagg
     3301 tgacttagga gatgatatag ggcaagaaaa agcaacaggt gtaatgaatg atataaaaac
     3361 agaaattgta aagaatggta caaaagatac caatcaaata gcaaatacaa taaataatgt
     3421 tgtaaataat tacaatatta ctattacacc agaacaaaaa gaacaaatta aaggtgtaat
     3481 gaaaaaaata gctgaccaaa attacgatta caatagcatg aaaaatacac tagataatgt
     3541 atcagataat gtaaatgaac aattaaagaa attaggtgaa agtgttaaag gttctggtat
     3601 actagatact attggtggat ggtttagtgg aataggagac tggttctctg gtttattctc
     3661 aggcggagat aaaaaagatt tgggaatctt aaataacact aatgatgagt ctcttggctc
     3721 tgatgctgta ataaattcta cagaaggtgt tcaaataaat ccacaaaata atactaattc
     3781 taacaatgat agtgatacaa atagctcaaa aaatgatact tcaagtaata atgaaaataa
     3841 cgatagttct caaacaaatg aagacaacac aacaaataat tcagaaaatc aatcaaataa
     3901 taatgactca gttggtttct ttgaaaaaat aaaaaattgg ctttcaggct tattttagaa
     3961 attaaacaaa taaaaagaat tgcctaaaat ggaaattttc tataaaaggc aattctcttt
     4021 atttacaata aatttttatt actcactcat ttttttagat ttttctatac atgcacatat
     4081 tgcttttatc acagaagctc taaaaccttc ttcttcaagt actttaactg cttctatagt
     4141 agttccacca ggtgaacata ccatatcttt taattcacca ggatgttttc ctgtctcaag
     4201 taccattttt gcagaaccca taacagcctg tgatgcaaac ttatatgctt gtggtcttgg
     4261 cattcctgca attactgctg catctgttat agcttctata aataaaaata catatgctgg
     4321 tgcagaacca cttgcaccaa ttacagcttc tataagatac tctgggacta cctcagtatt
     4381 tccaaaagaa ttaaatactt ctgtaactgc ttctaagtct tctttgttta tatttttatt
     4441 tatagaaaga gaagacatgg cttcatttac aagagctgga gtatttggca tagttctaac
     4501 tattttttta tcttctccta taatagactc tatatctttt atagattttc ctgctgctat
     4561 tgtaactata attttattgt catctataaa gtcttttatc tcttccaaaa tatcatcata
     4621 tatatctggt ttaacagcta caattactat atcagaggtt ctagttactt ccttcgagtc
     4681 ttttgtagta tttattccaa aatttgcatc tattctatcg agagttcctt gactatatgc
     4741 tgatgctata accatattag gttcaactaa ttttgaattt actattcccc ctatcatagc
     4801 gcttgccata tttcctgcac ctatgaatcc aatttttttc actttagtac ctccttattg
     4861 actaatagta atcgttaaaa tttaagcttc tttaactgca tcagactttt caaatggatt
     4921 tggtatactt actaccaaaa ttttcactac cttattagta tagttgaccc aattatgatt
     4981 gttcttttca ctattatagt gtattgtgtc tcctggatac atttctattt gttcatctcc
     5041 caaaaacact gtaagagtac cctctaatac atacacaaat tcctcacctt catgtacgta
     5101 acaacaaatt tcctcactac tcttacttgg aagaatttca attaatcttg gtagcattgt
     5161 tttttccttt aaactagatg ataaatgata atgtataaat gttgagtttt ctacatcaaa
     5221 tacttctttt tcataacttc ttaatacagc tcttttatgc tcttttggct tcataaaaaa
     5281 gtaagttaat tctacatcta gtacacttgc tatttttcct aaggaatctg ttgccacact
     5341 tgttaatcct ctttcaagtt gtgataaaaa accaattgac aagtttgttt tttcactcat
     5401 atccttcagc gtcatttgct tttgagttct gagttgcttt atttttgctc ctatgtcctt
     5461 attcaaaacc atcaccccta aataatttta attttttcat aatataaata caaaaatata
     5521 aatataggta ctgaacctat ctttatttca tttaaaaaaa tttttataag tattactata
     5581 atttatcaat ctttttttgt ctacattttt acgattattt acgatttatt tttcacatat
     5641 gtatatttaa tcttgccaac taaagataca ttttttataa gcatttactc tttatataat
     5701 aaaacttaag ttgttcatgt agtcttcttc tggtttaatt attaaaattt caaattatat
     5761 tattatttct acacattatt acatccttta gtacaattat aacataattt tacaatttaa
     5821 tgatatctat tcaatgtctt tttttactct ac
//
LOCUS       DAEORZ010000195         5673 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEORZ010000195 DAEORZ010000000
VERSION     DAEORZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.199"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            237..1064
                     /locus_tag="KRH39_003504"
     CDS             237..1064
                     /locus_tag="KRH39_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG8513376.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2087..2551
                     /locus_tag="KRH39_003505"
     CDS             2087..2551
                     /locus_tag="KRH39_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8513377.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFISLLDEGSSVFFGIGMKPNYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLRDGEFFVMRYQ"
     gene            complement(2818..3462)
                     /locus_tag="KRH39_003506"
     CDS             complement(2818..3462)
                     /locus_tag="KRH39_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513378.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3392..4681)
                     /locus_tag="KRH39_003507"
     CDS             complement(3392..4681)
                     /locus_tag="KRH39_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513379.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDHILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLEALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4678..5550)
                     /locus_tag="KRH39_003508"
     CDS             complement(4678..5550)
                     /locus_tag="KRH39_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513380.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         2061 a          778 c          700 g         2134 t
ORIGIN      
        1 attttaacat gtccataaaa tataataccc acaaatgata aataatactc aaaatatcta
       61 ttgaaataac tatacatcct taaataactt aacttaaatc taaacatttc aaaaacttct
      121 taaaaatatg taaaaatttg atgataaagt agcgattttt tcaaaaaaaa tgtaatatag
      181 agttatatct tataatattt tagagataaa ttttatcata gtggggagaa atttgtatgg
      241 atttaagcaa aaaagtaaaa attttagaaa atgcatcaga tggcaaaagc gaatttgaga
      301 tattagaatt tgaaaaactt gagggagctt ctagcccatt aatggcaatg ggattatatt
      361 ttgctagaga agctggtttg aaagctagac aaattaagat aaaattaaat aattcatcaa
      421 taaaaacaga agctggtgca ttatactatt ataaaggaaa tatatcttct gaaactaaga
      481 ttggtggtgt tggaggtctt tttaaaaaag gaatatcagg agcacttact aatgaatcaa
      541 tgatgaaacc tgtatacaaa ggtactggag aagtttattt ggaaccttct tttagacatt
      601 atttatttat gagtcttgat aatgattcta taattgttga taaaggatta ttctattgtt
      661 gctctgatac aatagaagta aaagcattta cacaaaagaa cttctcatct gctttacttg
      721 gtggtgaagg cttcttccaa atagaattaa gtggtactgg tgtaatcgtt ctggaatgtg
      781 ttgtacctca aagtgaaatt gtagagtatg aattgaaaaa tggtgaagaa ttaaaggtag
      841 atggtaactt tgcaatagca agaacatctg gtgttaattt ttctgttaca aaatcagata
      901 aatctttaat tggttctgct ttaaatggtg aaggtttctt aaatacattt actggtaatg
      961 gtacagtttg gctagctcca actcaagtaa tgtatgaaaa actaagtttt ggagcaatgc
     1021 ctactaatga taatatgaat aataaagttt ctaagcaaaa atagttgttt tataaatatt
     1081 tattaaatca ttattgattt tataaagtca ctataaattt tattagatta ttattataaa
     1141 atcagagtgt ggaaatgttt atgtgtattc tttaaataaa aatttccaca ttctgtaagg
     1201 ttattatact tgatatataa aattcaattt aggaacatag tctaacattt ataaacatat
     1261 taaggtaatg atttctctta gtaattattc tcatatctta ttctttgatt atatttatct
     1321 tattcctagc ccttatgtca tattatattt cttatcttat tatttataat tagcattaac
     1381 ttatctaaaa tcttgtattt ttttaatagt aaagtttaca tctatattaa aaagtttaca
     1441 tctatattaa tttcataaaa caatcaaggt agtcaagaga ttcaacttta tttgatttta
     1501 aaaatttaag tgaatttgtt cttatacaca cctttttaaa attatatctg tagaaaatat
     1561 catttagagc tataattaaa ttaacttatt aaattttaga aaatttatta aaaaatataa
     1621 gttaaattaa tatttttaaa gattcttttt tgtaactact agaatactaa tttagtagtt
     1681 attgtatctc taaagtttaa gcatggatag atatacattt gataatttgc ctataattta
     1741 cctactactt ggagtattgg agcaaataca atatattaat aataagttcc taaagccaat
     1801 aaattaacta atacacataa agatattgat gttgattata caactataag aaacggattt
     1861 aacagaattt aatcaagcta agaaattata tatatcgtga tgtttttgaa ttagaaggta
     1921 attcaaaatt agtttatagt cttctgtcaa tccaataaaa agtacagggt acaagaattg
     1981 ttagtaaaat ttttaaagat tttatggata aatataaata gttttactta ttgtctaaaa
     2041 ctttaaaata ccattaatta ccttaaatat tttaatagga ggacatatga atttaaataa
     2101 agttaaattt actgaagaac atgctattga agttactaat tggaaatatg aaggtaaata
     2161 ttcaatatac aatctacctc catgggagga aattaaaaag aagaattttt cactagcaaa
     2221 agaagataaa agaaaaaatt ttataagttt tataaatgac aataaagaat tgataggttt
     2281 tataagctta ttagatgaag gaagctctgt attctttggt attggtatga aaccgaatta
     2341 ttgtggaatg ggaattggta aagaaataat tagcctagga ttacaagaat gtagaaataa
     2401 atatagtact aaaccaattg tattaaatgt aagaacctgg aacatgagag ctgtaaaatg
     2461 ttatgagtct caaggattta aaatagttga gactaaggta caaaaaacgc atttaagaga
     2521 tggagaattt tttgttatga ggtatcaata gtttataaca atagtcttat tgttaataca
     2581 acaattaaaa taaaaaaata catatgtgta ggccatagca ataagtgtct ttgaaatata
     2641 aatttgagag cttttgataa aattttgtat acatatatat tttatactac agatatataa
     2701 atctatctac atacataaag agattgtaag taggtttgtg tatataaaaa tagatttata
     2761 tttcataaat gtacctttta tttgtattaa ctttatttta tctaagctct cattttatta
     2821 tctaatcttc ctttttactg gttttattaa gagagatgca attatcaaca caacacttcc
     2881 aagtatcagc caatatttct catcaaaact accttgactc atagacatta taaaaaattt
     2941 tccaacatac ttgcctttag tcatcatatt taaaattaaa tatcctatag atatcatata
     3001 acctatgatt gtattattaa ataaatttga gaaaaacatt cctaaacttc ctataaacat
     3061 agctgatgcg aaacttccaa taatatactc aactactgga aaattacatc caagatatat
     3121 taaaacattt acaaaaccta tgattaatac cactatacct ataattgaca tcagtatcct
     3181 aattaattgt acaataactt gagataatgc ttttgaagat actatctcat caatttcttt
     3241 tgattgctct agcgaaaata caggtaccaa cattactatc cctattattg caacataatt
     3301 ttctaaacat atagctgttg aaattctgtc taaattagct actccttgta ttagaggaat
     3361 catacatagc aatataattg aacatattaa attagttaaa gtgcttaatt tgaagttttc
     3421 ttttattaga actaaacagt tttgaaaact ttgcccaaac atcaagattc ccccttcttt
     3481 tttgctcata taagaatata gctaacaaaa caagcaccaa tgcttccaag cttatcacaa
     3541 ttctattaat cataatttga ttgaagtttt gtataataat atctccattt aatacactat
     3601 tatgtcttat cattagtccc attgttgaat aatcaccttt gagtcctgta gaatttatac
     3661 tgtaaagcca ccaaattaat tgcacaataa ttgctactgg tgtatctgtt aatatagtaa
     3721 tactcattcc tacagctgta actatcataa gagttggtag tatccaccat aatgtatatt
     3781 ttataaatgc taagttatca atagatatac ctttatcttt agcaaagaaa atcaaaggaa
     3841 gtaaatcttt catacctaaa attaatacag gcaatatcat cataaagaca agtgcaaaat
     3901 atcttgataa aactattttg catgaagata cttgttttga ataaataatc tctctcattc
     3961 ttgaacgctt atctttaagt gacataaaaa ctgccacaaa tactggaaat atacttaaaa
     4021 ctatacctat ataatcgcaa aaaattcttg catacccatt actaacttta tctttttgta
     4081 ttgtatagtt gtaattcttc atagcttcat cataggtaac tggaactact ccaaatcttt
     4141 gtaacttgtc aaaagcatat gcagaccctc ctcctaaaat gtggtctact ctttgcatta
     4201 tttcttcaaa tctttcaaga gatactttag aataatctat ttttatactt tcatcagttt
     4261 tccaaagtga atttatttcg ctaatacttt taccagttat ttcttctata tatttttcta
     4321 tggtcttcgt tttattttgg tctaatttta tatacttaac tacccctgtt ggatacgttg
     4381 tatatgtgtt tttttgatat tctctaatta ggtttatagt tgcatttgga atgacctttt
     4441 ctgttttcat agattctttt tgaccatatg gatattgatt gtgtacagaa taatattcct
     4501 caatattctc tactttttgt ggagcaacta atggatttcc atcattagga atatttttac
     4561 ttacatcatt tgaaactgaa cttccaagtt gagaaatata gaaaaaaact aaaactacaa
     4621 taaaaatata atataccata ctctttaata tatttttaca ttcttttata aatagattca
     4681 tatattatct cctcccatta taagcatata gccatcttca atagttggat tacaatttac
     4741 tgctgtctca aaaggtttat catctgatat aatcctaagt atagcctttc caccatgagt
     4801 caacattcct ataactataa atctactttt tatattttca atgtcttttt tatctacttc
     4861 aatactatat actttccctt cagctagttt tgctaatgct tctgttgagc cattgtatat
     4921 gacttttcca ctattcaata tagctacgtt ttcacatgta gcttctatat ctccaacaat
     4981 atgtgtagat aaaataacga ttctatcatt tgcaatttcg catagtaaat ttctaaatct
     5041 tatacgttct tctgggtcaa gacctgctgt cggctcatct actattaaaa ctctgggatt
     5101 atgaattaat gcttgtgcta tcccaagcct tctattcata cctccagata aagattttac
     5161 tttcacattt tgataattag taaggtttac tttgcttaat aactttgcta taatttttct
     5221 tctttttctt agtggtatat ccgataaaac tgctaaataa tccattgctt tatatactgt
     5281 catgttagga tacattgaaa agtcttgtgg caaataacca accatatttc taatttcctt
     5341 agtattctca ataggaactc cacacatatg tattgaacct tctgtttttg ataataaagt
     5401 ggctaaaatt ctcataaatg tggtttttcc tgctccattt cttcctaaaa gaccaaacat
     5461 accactgtcg atttcaagat ttatattatc aagagcttgt tttttcccaa atcttttact
     5521 aagattggat actgctattt tcatttccat atataaatac acccccagaa acaattttat
     5581 agaattttat ttttataaat ttttactgta ttttcataat aaattctaaa acttaagata
     5641 ttatttataa atttctaaat tttttctaaa gat
//
LOCUS       DAEORZ010000196         5584 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEORZ010000196 DAEORZ010000000
VERSION     DAEORZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.200"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            132..632
                     /locus_tag="KRH39_003509"
     CDS             132..632
                     /locus_tag="KRH39_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBG8513381.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            689..1675
                     /locus_tag="KRH39_003510"
     CDS             689..1675
                     /locus_tag="KRH39_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG8513382.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYISFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSGKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1725..2447
                     /locus_tag="KRH39_003511"
     CDS             1725..2447
                     /locus_tag="KRH39_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBG8513383.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2476..4410
                     /locus_tag="KRH39_003512"
     CDS             2476..4410
                     /locus_tag="KRH39_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8513384.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4403..>5584
                     /locus_tag="KRH39_003513"
     CDS             4403..>5584
                     /locus_tag="KRH39_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBG8513385.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GK"
BASE COUNT         2331 a          426 c          963 g         1864 t
ORIGIN      
        1 aatgaattgg gaaaatagtt cgctaaaata atttgttttt ataaaattac tgtttaagtt
       61 tatagatttt gtataaaata aagttatgtt attaattttt ttagaaataa aacttttact
      121 aggagacaga tatgagaaaa aatagtatta atatagcact aactgcagca attacagcaa
      181 tctatgcagt tttaacatta tcattgggat ttataagtta tggaccaata caatttaggg
      241 tagcagagat tatgatgctt ttggcatttt tagataaagg atatatagta ggtcttacat
      301 taggttgttt tttagcaaat gtaataggac catatggtgt acctgatata atttttggaa
      361 cattagccac atttataagt gcaagtatga tttatattac tagaaagtta ggtaaacaga
      421 ataaaagtac tttagttata gcttctttat ggccaactgt tataaattcg attgtggttg
      481 gtctaatgtt aaatgtattc tttgggcttc cattaattct atctatgata caagtaggtt
      541 ttggagaatt tgtagtagta acaattgtag gagtaccatt tttctgcttt ttaatgaaaa
      601 aatataaaaa tgtcattaat attaaatttt aaatagtgta tagtaattta attaattgag
      661 agtattatta gtgaaaagag gtgtttattt gtcgaaggta aaaagattga ctgatgtttg
      721 gatatatatt aatgataagc aaaagtttac aataaaagag ttgtcagagg aatttaatct
      781 ttcagctaaa acaatacaac gatatttaaa agaattaaat aaaatgggac ttccaattca
      841 agcagaacaa ggtagaaatg gaggttatag ggtgctaaat aatagctata tacctcctgt
      901 tatatttaca gaaaaagaag ttatgattat actatttgca cttaagtcac ttcagattta
      961 taattataag ttaatggaaa tagaaattaa ttctataatg agaaaagtaa gttatgaacg
     1021 taaaagcagt ataaaagtag gtatagaaaa tattaaaaga tacattagct ttgtagctaa
     1081 tgatactaat gatggagatt ttaagagtag taatgtaact gagtttttta gagcttcttc
     1141 agaatctcta attttaaata ttaagtataa gtgtggtaat cagattttgg aaaaaaacgt
     1201 atgccctata ggtatatatt taaataaggg agcatggttt tcaccagcat atgattgtaa
     1261 aagtggtaaa ataatattag ttcatataga taatataatt gatataaaaa gaacaggaga
     1321 aagtaaaaaa atacatataa acttggaaga atggttacat gaagtatata acaatttata
     1381 tggaaatagt tcaactacta aaaaatttgg tgaaattatt tatctaaatg tattattaac
     1441 taaagaaggt gtttctaaaa taaaggacag ttcttggaat ttagatatgg agctaaatga
     1501 agatggtagt ggaataatta gtacattcat aaaggttgaa gatatagatt gttatgcttc
     1561 gattttattt tttattggaa ataatgctaa ggttatagag ccaaaatttt tgaataagct
     1621 actttatgat aaggccaatg agttaagata tttttatgaa gaaaatatgg tataatattt
     1681 agaaatgttg aaattaaata attattaagg agatatacgt tataatggaa aattttaata
     1741 agaatgctaa aataattggt attggagctg aaggtattaa tataataaat gaagtagaag
     1801 aaaaaataaa agcaaatatg gatatagaaa aaataaacat aaatcaagag attgaaaaag
     1861 aatatgttag aagtctttta gatggtgtag atatattatt tttgatatat tcatctgaag
     1921 ataaacatat tagagatatt ataaaagctg tttcatatat gtcaaacgaa agaagagttc
     1981 tatctatagg aatggattgt tcagagaaag aaaataaaga agatttagag cttgggagag
     2041 agtttaaaat caataatgat agtatattta aatttgttga tttaatgaat atcatggtag
     2101 agtctatttc tgattcttgc atgataaata tagatattac tgatttaaaa gaagctattg
     2161 taggagataa aggaattaag tattcttttg aagagtttga agatacaaag tcatatagcg
     2221 agatagctga cattttattt gacaggatgg aatatattgg tgaggaattt ataagtaaga
     2281 aaggaattgt atttgttgaa ggaagtccag aattttctat aatggaattg aatgatttaa
     2341 taagtaatat tcagagcaag gtagaagaaa gctatgaagt aatattttct ttatatataa
     2401 aggaaaattt aaatggaaat attagagtag gcttattgta taattagtat attttagata
     2461 ttaaggagag tgtatatgtc tgaaaatata agaggtacag agtattggtt aatggaagaa
     2521 tttttttatc aggaaacaga cacaccacag atttttagag aagaaagcat aattgcactt
     2581 ggatttgata taaaaataaa tttaaaaaaa gtattcagta tggggaaaaa atcagaaaag
     2641 tatattaatg agaattgtaa tgataaagat tttataaaaa agtttatgtc gattaaaaaa
     2701 ggagattatt ttttatataa agcatatcaa aatatagaag aaaaagttaa atgtaatgtg
     2761 tctattggca tagttgaaga agattttgaa aatggatatg aattaagtca tattttaggg
     2821 catacactac cagtaaaatg gatgcataat tttgatacag tttcaaattg taaagtatat
     2881 acaagtgaat tttataaatt aaataataat gatattagct catatttaga aattattaag
     2941 tcacaagata agaaattatt gagaaaaaga atagaagaaa gtgaaaataa taaatggtat
     3001 ttaaaaaact attattataa aaatgtagat aagtgtgtag atgatgaaga attatttatt
     3061 cagcccatta atctagaaca attagaaagt ctaaaaaata taaagacaga tgattatatt
     3121 ttattgtata atagcgagaa agtaatttgt gaaaatgacc atacaatgaa gagtctattt
     3181 ggcgtatgtc aagttatgga agggttcgat aaagagaaag taagtgcttt ggagggtgga
     3241 tatttttttc ctgttagatg gatagagtat gaaaaggaga atatagacgg aaatgcatgg
     3301 gttatatttg aaaataatag tatagttgaa aagtatgtaa aaagatttac taataaaatg
     3361 ccattaaata ctatacttta tggaccacca ggaactggta aaacatacaa tacaaaacag
     3421 atggtttaca atctagtaag tgaaaccagt ctaagaaaca gatactatac aggttactta
     3481 gattataaaa acaatgtaga attttgtaca tttcatcaat catatggata tgaggaattt
     3541 atagagggtc ttaagtctga tggtgaaggc aattttaagc cagaagatgg gatattaaag
     3601 gatatatcta tagaagcatg ttataatgca ttgaaatttg aaaaaaagat gagctttgag
     3661 ttggaaaaag atagcttgac tttagaaact ataaaagaca ggaaaaagaa attagtatta
     3721 gaaaatatag aatatagtga gagttttaat tttgaagaat cagaaaacta tgttttaata
     3781 atagatgaaa ttaatagagg gaatgtatct aaaatatttg gtgagctaat aacattattg
     3841 gaagatgata aaagacttac taaatcaaat caaactattg tcaaactacc atattcaaag
     3901 gaaaaatttt ctctacctcc aaatttgtat atagttggaa ctatgaacac atcagataaa
     3961 tcaatagcac ttttagatat agctttaaga agaaggttta attttgaaga aattatgcca
     4021 gattatgatt tattgtctat tgtagatggt atagatatta gaaaaatgct atatacaatc
     4081 aataaaagga tagagttttt atatgataga gaccatgtta ttggacaagc ttatctactt
     4141 gctgtagaaa atattaatga tatagtagta gtatttagaa gtaaaataat accacttctt
     4201 caagagtatt tttattatga ttcagaaaaa gtaggtttga ttttaggagg aattggaaga
     4261 aatgaaaaag ataaatacat tgtttataaa gaaaaactta tagcttctga gttatttaaa
     4321 aataataatg cctcaataat ggagagtaaa gttaactatt gtataaaaca aaacatatct
     4381 agtgaggagc taaaaaatat atatgagtaa aaatatatat gttaaggaaa cctatgaatg
     4441 gataagggta ggtaatggtg aaaatgagct tacagaaatt gaatatgaaa aattgcttaa
     4501 gtatctagag aacaataatg atgtattaaa aagtaatata atagatatta aatacaaaaa
     4561 acttagattt ataaactatg taggcatcat atgttttgaa aatgtaatac ttgaaatact
     4621 tcctaaattg tcattaagtg ataatcttgt aaaagataga gagatactat tacagatgct
     4681 ttctatatgt aataaaattc caattacaat gaatgaaaaa ataagattga gtttgaaaaa
     4741 ttataatctc ttaaattttt ttgttatgta tttcattgag agtatgcaaa cacagatgaa
     4801 aaaaggtatt tattttgaat atataaataa aatagagaac ttaaatgtta tgaggggtaa
     4861 aatattatta agtacatatg caaaagaaaa aggcatttct ccaatgaaaa taagatgtga
     4921 atatgatgag tatagtgaaa ataatttctt gaatcaagtt cttaaaaaag catgtatatc
     4981 tattttatgt agaataaatg acaattcaat tcaaggcaag attaaaaaaa ttttaagcta
     5041 ttttcaaaat gtagatttaa tttatataga tagaaaaaaa ctgttggatt ataaatttta
     5101 taaaaacaat gatagattta aagattgtta tttactagct agacttctac ttttaaattt
     5161 gtctatggat aatagtcaaa ataatcaaga ggcattttct atattatttg aaataaatac
     5221 tttgtatgaa gaatatatag gaattttaat caaaagtata tgggacaatt catttagaga
     5281 aacatatata caagataaga gtaaatttct tttgaaaaat gaacaaacag gtaagaaaaa
     5341 ctttaattta agaccggata tagttttata tgacttaaaa aatgaatatg aaattataat
     5401 tgacactaaa tggaaagcta ttgaagtaga ttcaaatgtt ttttataggt caagtgatat
     5461 ataccagatg tatgcatata taacagcata tgaaaatgca aaaagatgta tattattata
     5521 tccttgtatt caaaaagata agaattatag ttcctggaaa ttatcagaat catttaaggg
     5581 taag
//
LOCUS       DAEORZ010000197         5561 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEORZ010000197 DAEORZ010000000
VERSION     DAEORZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.201"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(49..945)
                     /locus_tag="KRH39_003514"
     CDS             complement(49..945)
                     /locus_tag="KRH39_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8513386.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASHVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYKNEHREVSNIILKEDMK"
     gene            1087..2514
                     /locus_tag="KRH39_003515"
     CDS             1087..2514
                     /locus_tag="KRH39_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8513387.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQIGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSDLILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2711..5305)
                     /gene="clpB"
                     /locus_tag="KRH39_003516"
     CDS             complement(2711..5305)
                     /gene="clpB"
                     /locus_tag="KRH39_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBG8513388.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5342..>5561)
                     /locus_tag="KRH39_003517"
     CDS             complement(5342..>5561)
                     /locus_tag="KRH39_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513389.1"
                     /translation="KSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDFFNSDGTFNLT
                     LSFDIQKNENLLDSLLELRKSIIINERI"
BASE COUNT         1752 a          912 c          614 g         2283 t
ORIGIN      
        1 aactcaaaga tttaaaaata tatattttct ataaagtata tttatgaatt atttcatatc
       61 ttcttttaaa ataatattac taacttctct atgttcattt ttatacttag ttggagatat
      121 attataattc cttttaaata agcgactaaa ataatttatg ctatcaaagc cacatctcat
      181 agcaatttct gtcatattta aatctgtttc ctttaaataa tatatagctt tatcaagcct
      241 gattccattt ataaaatcga ttgtagtctt acctgttatt tgcttaaatg ttctacaaaa
      301 gtggtgtgca ctcaagttca catgactagc aagttctttt aatgtaattt tatccatata
      361 tttttcttct atcaaatcaa agatacattc aaatttttct aaagaatact ttctttttat
      421 aaattcatct tcagaatata ttttatcaat atgacttctt aataagagta ctattaattg
      481 atagatatta gcttttatag ataactcata tccaacttct ttattgtgat attcatcaag
      541 tatagttgta atacatttta aaatcagaga atcattttga attaaattat taaatacaat
      601 atgatttaaa gccaatggtg ctaagtactt ggtttgtaac aaatcaactt gattactaaa
      661 aaggaatgtg ggttcaatac gaatactata gaattttaaa ttatctgata agctttctaa
      721 ataatgtaat tcattactgt ttgctattac aatatttcca ggcactacct taaaattatc
      781 caatccacat tcaagatatg ctataccatc aataaaataa tatatttgta gatgctcatg
      841 ccaatgtctt ttaaagtttg ctcctttttt aatgtcatcc tttaaaaaaa tatctataaa
      901 gttttcttga ccttttctgt taaaatatgt atttttatcc ttcattattc ccccctcatc
      961 aaaatagtgc taattataag caatatagtt attgtattca ttatttcata tcgctattat
     1021 ataaaatgta actatcattg taaacattaa cataatgcca gtcattataa taaggaggta
     1081 ataaaaatga caaactcttt taaaaaagat gataatagaa aaaaaatcac agttttattt
     1141 gtagtcattg ccatgacttt tatggcaaca ttagattcta gtattataaa tgttgctctc
     1201 ccagttcttg caagcaaact aaatgtatct ctagcatcta ttgaatgggt aatagcaagt
     1261 tactcaatta ttatttgttc aacactatta ttttttggaa gacttggaga tattattggt
     1321 aaatcaagag tatttcaagt aggtactatt ctctttacat cagcttcatt actatgtgga
     1381 ttaagtaatt ctttaacttt acttatagtt tgccgcttta ttcaaggaat aggcgcttct
     1441 gcctacatgg caaacaatca tggaattatc acagaactat ttccaaaaga aagcagagga
     1501 aaagccctag ggattttagt tactgcagtt gctattggaa atatggttgg accatctgtg
     1561 ggaggattta ttttgtcaat atttgattgg aatgttatat tttttataaa tattccaatc
     1621 ggtcttattg taatcttttt aaatacaaaa tttcttccta atagtaaaaa atcttcagaa
     1681 aatatggata aaactggtgc tatacttcaa tttttaggaa caacattatt ttttagtgct
     1741 ctaattagtg ctcaacaaat aggattacta aatccatata tattaattgc actattatta
     1801 tcaattattt ttattatatt atttttgata ttagaaaaaa aacatcctca accgcttctt
     1861 gaccttgaaa tttttagaaa ttttaaattt tcattgaatt taatttgtgc tcttacatca
     1921 ttcatttgta ttgcatcatc aagtatttta attccatttt atttacaaag tactatgaaa
     1981 cttcccccta ttcaagcagg tttatttatg atactatcac ctttgatttt agccattttt
     2041 tcacctatat ttgggaatat atcagataaa attaagtcag aaaaaattat tttaattggt
     2101 cttctcgtta tgagttttgg attctttcta atgtcaagat tgaaagaaag ttctgacctt
     2161 attttatttg taatttatat atcgataata tcaataggac aagctatatt tcaacctgct
     2221 aacaatgctt tgataatgtc aagctgttct agaagtaagc taggagttgt aggaagcata
     2281 aattcccttg taagaaattt aggacaagtt ataggtatta ctatttctac aacactttta
     2341 tataatttta tgagtattaa agctggatat agggtcaatg attatgtgat taataatgat
     2401 aaaatatttg tatttggaat gagaaatgtt tatattattg ttacacttgt ttgcttaata
     2461 ggtgcaatat taattggctt ttatttattt aaatataata aaaatgaaca atagtttttt
     2521 ataattatat tcaaattatg attatattat aagaaactta ctaaaatatt tcctttgagt
     2581 taaatttaat ttggacatat tgacttaaaa atgttttatt agtttgataa aatatttctt
     2641 ttcctatagt ttataaataa aaatcagctg agcaaaatat ttagtatatt atgctcaact
     2701 gattctttta ttatttaact aaaacttcaa tattttcatc ttttccatct ataactatag
     2761 tacatccatt atacacatct ccagcaataa gtttctttgc aattatagtt tctaatgtat
     2821 ttcctatata tctcttaaga ggtcttgctc catatacagg gtcatatcct tctctcacca
     2881 taacatcttt tgctgaattt gtaacttcaa tagtaatatc tttttctttt aatctatttt
     2941 ttaaatcttt cataaatata tctataattt tcttgatatt ttcttggtct aaaggcttaa
     3001 acattataat atcatctact ctgtttaaaa actcaggttt aaatctatgc ttcatttcat
     3061 tcattactaa attgttagta gtctctgtta tatttcctcc tgcttctaat agataattac
     3121 ttcctatatt agaagtcatt attataagag tatttttaaa gtctactgtc ttacctttat
     3181 tatcagtaag tctaccatca tctaatatct gtaagaacat gttaaataca tcctcatgtg
     3241 ctttttctat ctcatcaaat agtattacag aataaggagc acgtctaact gcttctgtaa
     3301 gttgaccacc ctcttcatat cccacgtacc ctggaggagg tccaactagt cttgatacag
     3361 catgtttttc catgtattca gacatatcta ttcttattat attatcttca ctatcgaata
     3421 aatttctagc aagagttttt gcaagttcag ttttaccaac acctgtaggg cctaagaaaa
     3481 taaatgaacc aattggtttt ctttcatctt ttaatcctgc tcttgcacgt attactgcat
     3541 ttgaaactgc tgtaactgct tcatcttgtc caataactct tttatgaagt tcatcttcta
     3601 gtttaagtaa tttttctctt tctccttcaa ctagtttagt tactggtata cctgtccact
     3661 ttgatacaat gctagatatt tcttcttcag taacttcttc ttttaaaaga gaattttcag
     3721 aaccgtcatt catattttct tcgtactgct ttatttgttc ttctaactta ggtatttcac
     3781 catatttaac ctctgctgct ttgttgaaat catattctct ttcatacttt tcagctttac
     3841 ctttagcttc gtctaattga gcctttaagt ttttaatatc taatatttga ctcttttctt
     3901 tttcatattt tgctgtcatt tcatcatttt tagattttaa ctctgctatt tctttttgaa
     3961 tatcatccaa tcttgattta cttttatcat cattttcttt aagtaatgct tctctttcag
     4021 tttctaatgt aaataacttt cttctaacaa catctaattc tgttggcaat gaatctattt
     4081 cactacgaat catagctcca gcttcatcaa ttaaatcaat tgctttatct ggtaaaaatc
     4141 tatcttgtat atatctatga gaaagttttg ctgctgctac aatagcatta tcatgtattc
     4201 ttactccatg atgtatctca aatctttctt ttaaacctct aagtatagat attgtatctt
     4261 caacagtagg ttcttctgca attacaggct gaaaacgtct ttcaagagcc ttatcttttt
     4321 ctatatactt tctatattca tcaaaagtag ttgcaccaat acagttcaat tcacctcttg
     4381 caagcatagg tttaataaga ttacctgcat ccattgaacc ttctgtttta ccagctccaa
     4441 caatagtatg aatctcatct ataaacagta taatttttcc atcagaactt tgaacttctt
     4501 ttaaaactgc ctttaatctt tcttcaaatt cacctctata ttttgcacca gcaattaaag
     4561 ctcccatatc aagtgagaat acaatcttat cttttaaacc ttctgggacg tctcctctta
     4621 ctattctctc tgctaaccct tctacaattg cagttttacc tacacctggc tcaccaatca
     4681 atacaggatt attttttgtt cttcttgata aaattcttat aatccttctt atttcttcat
     4741 ctctcccaat tacagggtct aatttatttt tcttggctaa atctactaaa tttgttccat
     4801 atctagcaag tgcatcatat gtgccttctg ggtcttgtgt ttctactctt tgactacctc
     4861 tcacttgtga taaaatattt aaaaagtcat tcttatttat attatattgt tttaatattt
     4921 taccaacagc tgattttgat tctgtttcca tcattgcaag cataacatgc tctacactta
     4981 tatatgaatc tttaaattct ttagaaatac tctctgcctt tataagcacc tcatttattt
     5041 ttctagtagc tgatactcct tgtgagtcag caccttctcc atatactttt ggaagcttat
     5101 caatttcaaa gtttacagta tttctcaaag aatcaattga tatattcatt ttttcgatta
     5161 tatttggtat caatccatcc tcttgattaa taagtgctga gaaaagatga attacatcta
     5221 cttgttggtt atgatttttt actgcttcgt tataagcctc atttaaactt ttttgaactc
     5281 ttaaagtcat tttttctacg tccatataaa tccctccatt ttaatattat atccaaataa
     5341 attaaattct ctcatttata attattgatt ttctaagctc taaaagacta tcaagtaaat
     5401 tctcgttttt ttgtatatcg aaactcaatg ttaaattaaa tgttccatct gaattaaaaa
     5461 aatcgaatac ttcttttgat tttgctatta aatacaagtc ttcatctata agtctttttt
     5521 cacctattaa taaatactga catttatcta taaaactctt t
//
LOCUS       DAEORZ010000198         5530 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEORZ010000198 DAEORZ010000000
VERSION     DAEORZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.202"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            232..1437
                     /locus_tag="KRH39_003518"
     CDS             232..1437
                     /locus_tag="KRH39_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG8513390.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1508..1729)
                     /locus_tag="KRH39_003519"
     CDS             complement(1508..1729)
                     /locus_tag="KRH39_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513391.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(1870..2469)
                     /gene="recR"
                     /locus_tag="KRH39_003520"
     CDS             complement(1870..2469)
                     /gene="recR"
                     /locus_tag="KRH39_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBG8513392.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2482..2832)
                     /locus_tag="KRH39_003521"
     CDS             complement(2482..2832)
                     /locus_tag="KRH39_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBG8513393.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(2891..4528)
                     /gene="dnaX"
                     /locus_tag="KRH39_003522"
     CDS             complement(2891..4528)
                     /gene="dnaX"
                     /locus_tag="KRH39_003522"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBG8513394.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4605..4869)
                     /gene="ffs"
                     /locus_tag="KRH39_003523"
     ncRNA           complement(4605..4869)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KRH39_003523"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4907..4998)
                     /locus_tag="KRH39_003524"
     tRNA            complement(4907..4998)
                     /locus_tag="KRH39_003524"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4962..4964),aa:Ser,seq:gga)
     gene            complement(4999..5454)
                     /locus_tag="KRH39_003525"
     CDS             complement(4999..5454)
                     /locus_tag="KRH39_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBG8513395.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
BASE COUNT         1758 a          984 c          633 g         2155 t
ORIGIN      
        1 ttctatgctc tattttcctt tttattctat atttaaattt tttatttaca agaatatttt
       61 taatataaca tatttgataa aattaattta catttctttc acttgtaatt tttgcaatct
      121 tgtattagat taatattaaa aataatttat caataacctt attcacaaag acagttttta
      181 tatatcaaaa tataatataa atactatgct acattttagg aggattttat tatgagcaaa
      241 aaagtattaa taatgtctgc ttccactggt ggtgggcata atagggctgc tctagctatt
      301 aaagaagaac ttacttccaa aacacttgat ggagagccaa ttgaatgtga aataatagat
      361 agtttgaaat tagttaataa tacaatggat aaaataattt caagaggata cgaaaaatct
      421 gctatatata ctcccaaagc ctatggaagt gtatatagat tatctgaaac aaatcttttg
      481 tcaaaaaatg aatttaaaga taatctacta ataacattca tggccaaaaa atttaaaaag
      541 cttattagaa gcgaaaaacc agacttgatt ataggaacgc atccttttcc aatgattgct
      601 ctaagtacat tgaaaaaaaa ctttaattta cataataatg aatcaaatgc ttacacagag
      661 catttttata aacattatac aaataccatt aatgttcctc cgcttatttc tgtattaacc
      721 gattatacta ctcattctac atggatacaa aatgaaattg attactatat tgttggtcat
      781 gaatatgtaa aagaattatt ggtgtttgat ggagttgaac cgagtaaaat tagaacattt
      841 ggaatccctg ttgaaaaatc ttttctctct cacagagata aagatattgt tctttctgag
      901 ttgaatttat ctcctgataa attaactgtt ttacttatgg ggggtagctt tggggctgga
      961 aatataaaag agacacttga tgaattactg gatactgata gagactttca aatacttgta
     1021 attactggta agaatgaaag cctaaaagaa aaaatcgaaa aaaaacttat gtctcgttat
     1081 catgataaaa atgtatgtgt tcttggatac actgataaga tgaatgatat tttagcttct
     1141 atagatgtat tagtaagcaa acctggtgga cttactacaa ctgaggctct tttaaaagat
     1201 gtacctatga tagttcctta ttatatacca ggtcaggaag aagagaatct tgatttctta
     1261 tctaattgtg gtgcagcact tagaactaca aaaaaatata acttgtcaat attactaaaa
     1321 gttttaatag atgaccctag cagattagaa atgctaaaaa gaaatattaa atcaataaga
     1381 aagtcaaatt cagcccaaaa tatagctaat ttaattttaa atataatttc tggttgataa
     1441 aataaataaa atccctatag tatatatatt atctattagg gattttttat tttctaatta
     1501 tttaatatta aattggttca taaaagtctt cttcataatc atactgataa tttttataat
     1561 tatttctaaa ttcagcattt ttatttcgta catttttttt agtttttttc ttattagaaa
     1621 aacttatatc ctcaaaatca tcatatttag gtttatcttt tgatttcatt tttttaactc
     1681 ttttttcttt cattgcatta tgttcttcta agttcataat tggtttcatg ttgcacccct
     1741 atcaaaattt ttaaatgatt tatacaacaa attatacaat acagaagaaa tttattcaat
     1801 aaatctaaat caaaattaat ttgaatttaa ctcaaaaaat tacaaaaatc gaggtattaa
     1861 cctcgatttt tatatttctc ttctgccttc tagtgctttt gatattgtga cctcatctgc
     1921 atactctaaa tcgcctccaa caggaagacc atgagctatc cgagtgactt tgattcccat
     1981 tggttttact aacctagcaa tatacatggc tgtagcttca ccttcaatag taggatttgt
     2041 tgccattatt atttctttga cttcctgatt gccaagtctt tggataagtt cctttatttt
     2101 taacatatct ggtccaattc catccattgg agaaattact ccatttaata catgatactg
     2161 tcctttaaac tctctcgttc tctccatagc tgctacatct cttggatctt ccacaacaca
     2221 tataacactt gaatctctac ttttatttga acacatgcta catgggtctg tatctgttat
     2281 attacaacaa attgagcaat atcttatttc tctttttgca tctataatcg ctttagataa
     2341 agcttccaca tcattagtat tcatattaat aatatgaaaa gccaatcttt gagcagtctt
     2401 tcttcctact ccaggaagct ttgaaaattc ctctataagt ctcgttatgg gacctgtata
     2461 aacttgcata aataccacct actagaataa cccaggtata ttcatacctc cagttacctt
     2521 actcatttga cttgcttgta tatcatcaat atttctcaat gcttgattta ctgcacttaa
     2581 aactaaatct tgtagcattt ctatatcatc tggatcaact acttctggct tgatagttat
     2641 atctataact tcttttttac cattaacttt tacagtaact gctcctccac caactgaagc
     2701 ttctacttct ttactttcta actcttgttg agctttttgc atattttctt gcattttttg
     2761 agcctgcttt aacaagttgt tcatatttcc accaccaggc atcattccgc ctccaaaacc
     2821 ttttttagcc ataaacacta ttcctcctaa acttattttc attttattat tatatcataa
     2881 tagtatttta ttatttactc tcattttcat caatactatc ttttatttct aatatctctt
     2941 cactcactat gttctttaat atttcctcac caatgtcctt tttctcttta acttcaagat
     3001 ttatatcttt aacttctgat gtcagtacta tttttacatt aaaacttcta tttaaaacat
     3061 ctcttattgt tttttctaaa taatttattg tatcttgaga gctcagtcta gtttttgcaa
     3121 aatcaaattt atcatcaaat ataacataca acatattaga atatacatta aaacttttta
     3181 cttcacttaa gagtgcgtat attggcattt ttttatcttt tttaatttgt ttaagtatat
     3241 tcttccaaga tgactctata agtataacgt cctcattttt tacatcttca taaactacat
     3301 tttcttgttt tacatctatt tctctatcga cctcatactc tttttcttta ttattcccaa
     3361 ttttttcaga cttaaagttc cccagttcaa tcattttttc taagttctca actcttttta
     3421 taagagcttc tttactttca tcaaacattg gttgagcaat tttcattatg gttatttcca
     3481 ctaaaaccct tggatttgaa gatgacttta tatcatcctg agttattgat aatatattta
     3541 aaatccttat taaatcattt atatctatat ttttagactg aattttaagt tgttctatag
     3601 tttcttctgg taatgaaatt atttcatcca attctgatga aaccttacat accattaaat
     3661 ttctaaaatg atcaattaag tcatttataa gatttcttat atctttgccc cataagacaa
     3721 attcatttaa tatctcaaga gactttttag tgtcctgctc tacaatacat tgagacaatt
     3781 caaataattg ttctatattt acgcttccca ttaattcaac aacatcttta taatctatct
     3841 tactttctcc aaaagacata cattggtcta atatactaag tgcatctcta agagctcctt
     3901 gagaatttct agctattaaa ttaagagctt tatcatctat atcaattcct tcatcttcac
     3961 aaatcttttt cattctaagg gtcatatctt taactgtaac tcttttaaaa tcgtatcttt
     4021 ggcatcttga taaaatggtt gcaggtattt tgtgaggctc tgtagttgct aatataaaaa
     4081 ttacatatga tggaggttcc tctaaggtct ttaataatgc attaaaagct ccttgagaca
     4141 acatatgaac ttcatctatt atatatactt tatatttagc attggctgga gaatatttca
     4201 cactttctct aagctctctt atatcatcta cactattatt tgaagctgca tctatttcta
     4261 caacatccat tgtattatct tttaatacag attcgcatat ctcacactca ttacacggtt
     4321 cttcatttat tttattcaaa cagtttactg ctcttgaaaa aatctttgca gtagatgttt
     4381 taccagtacc tcttgttcca caaaacaaat aagcatgtcc tatattatca ctatatattt
     4441 gattctttaa agtcttaata atatgatctt gaccaataac atcttcaaat ttttgtggtc
     4501 tatatgctct atataatgct ttatgcatct ttttacctct ctttagcaaa attaataaaa
     4561 aaatccctca ttatctaagg gattagtctt ttatatatta aatgctgtac accatctatc
     4621 gacttaattc cataggcgtt accaaaacag ttagctcaac ttaggcaccc ccacggcaca
     4681 caaaagtgat cacttaccgc tgcttccttc cggatctgac ggggttcatg agctttcatt
     4741 gcgcaggacc taagcatcaa caccacttat ttagggcggc cctacagaaa ataagccttt
     4801 agatggaatt caatcctgct atagcggatt gcaggttaca gggcaccgct acctccccat
     4861 ctagtacagc aaaattacaa ccatatttaa ttacttttat tgtttctggc ggagagagtg
     4921 ggatttgaac ccacgataca gttttaccca tatactcgct ttccaggcga gcgccttcga
     4981 ccactcgacc atctctcctt aatctctttt tctcaatgcc ttaaaaaatt cttgcaatat
     5041 gatcgagcat tcttcttgca aaacatcaaa agctactttt acattattat tattaaaata
     5101 atctaattta aactcatgct gtttttctat atatgaattt tttacatgtc ttgtccctat
     5161 tataagcttt tttattctag aattaactat tgcaccacta cacattatgc aaggttccat
     5221 tgtaacatat aaatcacaat cagtcaacct ccaaccacct aatttttctg atgcttgttt
     5281 tatcgctaaa atttctgcat gtgccgtaga gtcttttaac gtttctgtaa ggttatgagc
     5341 tctagctatt atttgattat ctttaactat aatagcacct attggtgttt cttttttatt
     5401 atatgcttta tatgcttctt ttaatgcttc tttcatataa aatgaacttt ccataattcc
     5461 tcccaaaact tataacaaac ttatcttatc ataattcatt ttataattca acttaatttt
     5521 attttgttta
//
LOCUS       DAEORZ010000199         5509 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEORZ010000199 DAEORZ010000000
VERSION     DAEORZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.203"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            191..1027
                     /locus_tag="KRH39_003526"
     CDS             191..1027
                     /locus_tag="KRH39_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG8513396.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1380..2957
                     /locus_tag="KRH39_003527"
     CDS             1380..2957
                     /locus_tag="KRH39_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG8513397.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2950..4134
                     /locus_tag="KRH39_003528"
     CDS             2950..4134
                     /locus_tag="KRH39_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG8513398.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPSEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEESEFYLRLNVACSREKLEDGLNRVKKTMDYIAKR
                     NL"
     gene            4152..4751
                     /locus_tag="KRH39_003529"
     CDS             4152..4751
                     /locus_tag="KRH39_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG8513399.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4775..5245
                     /locus_tag="KRH39_003530"
     CDS             4775..5245
                     /locus_tag="KRH39_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8513400.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         2071 a          569 c         1041 g         1828 t
ORIGIN      
        1 aatatatttc aatgaaaata tgataaaata tgacaaatta attgatgaca ataaaaaata
       61 ttttgttatt gtagtattat agggtaaatt gcaaaatata tacgaaagta aaattttaaa
      121 tattgacatt aaattaaata tactatacta tgtaagtgtg atgagagaat ttttttagag
      181 gggatttata gtgaatataa aaaagatatt taataacaat gtagtagtaa gctccctaga
      241 agatggcaca gaaataattg tcactggtgc tggagtagga tttaagaaaa aagtaggcga
      301 tttgatagat gaaaatttga tttctaagaa atattttgtt caagacgatc aaagggacaa
      361 atacaatcaa atacttaata agacctctat tgaatacttt aaaatttcag aagaaattat
      421 agaaaaggct aatgaagttt taaatactca agtgaatgat tcaataatat tagccttaac
      481 tagtcacata gaatttgctg tccaaagaga aaaacaaggt ataaaattac caaacttaat
      541 attgaatgag acaaaacaac tttataggga agaatttgaa tttggattat gggcaattga
      601 tgaaattgaa aaaaggatag gaataaaatt gcctgaagat gaagcaggat atattgctat
      661 tcacataatc aatggtttgg aaaattctca aaaagatgaa ggtattaata tactagagtt
      721 ttcaaaacaa gttattcaga taattgaaga agtacatgga tttaaattgg atgtaaattc
      781 attaaattat actagactta taactcactt aaagtttttt gttcaaagga ttctccgaaa
      841 agaaacttac aatgatagtg atattgaaga tatgtataag ttagtgaata aaaattacaa
      901 taaacctaaa gtgtgtactg aagaaattgc agcattggta ttagacagat ttgaatataa
      961 gattagtaaa gaagaagaat tatatttaat gattcacata aataaaataa caaacagtgg
     1021 ttgttaggat tgtaactgat aaagcaggca aaacctaagt cattgagctt aactattaga
     1081 ttaagttttt tgatttaggt ttttttatta gatacaatca aaatacagac agtgactttt
     1141 aggattgtaa ctggttaagc aggcaaaacc taaatcattg aatttaacta ttaggttaag
     1201 ttttttgatt taggtttttt tattagatac aatcaaatac agacagtgac ttttaggatt
     1261 gtaactggtt gagcaggcaa aacctaaatc attaaactta attattaaat taagtttttt
     1321 gatttaggtt tttctataaa tttgattatg tttttagata aaaataggag gggaaaatta
     1381 tgaaaaaaaa taagttaggt ttatggaact tcttccaaat gttaggaaaa acgtttatgt
     1441 tcccaatagc actacttagt gtttctggga tgatgctagg acttggggca ggttttacag
     1501 accctaagat gattgaaatg gttccatttt tagggaatga atatgttaac ttagttttaa
     1561 acttcatgtt aacaatagga ttatttgcat ttaacaactt gggagcatta tttgcaatgg
     1621 caattccact aggattatta aaaaaagaaa aagagtttgg tgctttttca ggattagtag
     1681 gatttatagc aatgcacata ggtacaaatt tttatttaac aagagctgat ttgttagtac
     1741 cagcagaaca gatgtcaact aatggtcaag ctatgattat gggtatacaa acatataata
     1801 caagtgtttt aggtggtatt gtagcaggat taattgttta tgcaatatat gataaagtta
     1861 gtaatttaaa gataccagaa tcattaggat tctacagtgg accaagatta gttccaattg
     1921 ttagtttaat agttatgagt atagttggat tattaatacc tattgtttgg ccaccatttt
     1981 tcaatgcatt ccaaagttta ggaagatgga tttcttcagc aggaccatta ggatattttt
     2041 catatgcagt ggcagaaaga gttacaattc catttggact aaatcatctt gtaacatctg
     2101 tattccgttt tacaccaata ggtggaactg ctgttattga tggagaaacg tactttggta
     2161 cattgaacat gtttatggca tatgtagaaa ataatcagat tatacctctt gatttagctg
     2221 gtaaaatgga acaaggtaaa ttgatgattc aatatggatt agctggtgct gcattggcta
     2281 tctaccgtac agcaaaacca gaaaatagaa aggcacttaa aggactttta atatcaggag
     2341 tgttgacagt tattataggt ggtataagtg aaccaataga gtttctattc ttattcatat
     2401 caccagcatt atttgctttc catacattta tgaatggtct agcaaacatg gtacttccat
     2461 acttaggcgt gttgatggga ttcacaggag atttaattgc atttataagt tttggtgtgt
     2521 taagaggaac agctacagga tggccaatag cagtattagt agcagtttta tattttgcaa
     2581 tatactattt tgtatttaag tgggcaatta ttaagtttaa tataaagacc ccaggacgtg
     2641 aggataaaga aataaaagta tctgaaggaa atggttcaga tttcaaaaat ttgtctacat
     2701 ataaaggaca acaaatgata tctgctttag gtggtcaatc taatatatta tcactagata
     2761 attgtgtaac tagattacgt ttaaaattac aagatgtatc attaataaat gaggatgcaa
     2821 ttaaagaggc tggtggtata gcagttgtta aattggatga gcatacaata caagtaatta
     2881 taggaactca agtgtattct ttaagaaagc agatggataa agctatggaa ggtcatttgg
     2941 aggcatgtaa tgattaattt tgataaaaaa gtagaccgat tgggtacata ttgtacccag
     3001 tgggactatg tagaagatag atttggtaag aaggatttgc ttccttttac aatttcagat
     3061 atggatttag agtttccatt tgaaataaca gaggtgttgc aggaaaggtt aaatcataga
     3121 gttttgggat acagtagatg gaaacatgat gattttagaa attcagttac gaattggtat
     3181 attaaaagat ttaattgtag cgttgatagt aactggatat attatagtcc aagtgtaatg
     3241 tattcaatta gcaaacttat tgagatgtta agtaatgaag gagatggaat ccttataaat
     3301 actcctgcat acaatgcttt ttatgatgtg ataacaaata acaatcgtaa tattataaag
     3361 tcacatcttg taaataggaa tggattctat tcaattgatt ttaatgattt tgagaaaaag
     3421 tgtaaaaaat ctaagatatt tattttatgt aatcctcaca atccaactgg tagagtatgg
     3481 agtgagttag aacttgttaa gatgatagga atttgtaaga ataatgatgt caagataata
     3541 tcagatgata tacacatgga tattgtatat gacaatagaa tgacacctat cttaaatgtt
     3601 gcaaaggatt atttagattc tgtattcatt tgtacatcag cgtctaaatc tttcaatata
     3661 ccagcattga ctgggtcata tgtgattata ccaagtgaag gtattagaaa taaatttgaa
     3721 aatatcacta gataccgtga ttttgtaaat tcgccagcaa ttctagcaat attagctaca
     3781 atggtctgtt atgatgactg cgaatattgg ttagatgaac tattaaaata ccttaaagag
     3841 aatcttaaat atacgattga ttatataaaa gataatttag aaccattgaa attggtcatg
     3901 ccagaaggct gttactttgc ttggatagat ttttccgaac ttaaaataag tggtgagaaa
     3961 ttccaaaaat tattgatgga tattggagaa gttgctatta tgtcggggtc tgtatatggt
     4021 gaagaaagtg agttttattt aaggttaaat gtggcatgtt caagagaaaa acttgaagat
     4081 ggtttaaata gggttaaaaa aactatggat tatattgcaa aaagaaattt atagtaggaa
     4141 gggagatagt tatgggatat ttaaaaacaa tggttgatgt tatgactact gaaatgaatg
     4201 ccataagatg tttaatagat gaagcaggaa tagaatatga aagtatagtt agtgaaattg
     4261 caaattgtga aggcaaagta atttttatgg gtgtaggaaa atctgcacat ataggtaaga
     4321 aattggcagc tacatttgct agtactggta cacctagctt ttttgtacat gcaacagagg
     4381 cagtgcatgg agatttagga atgattgaaa gtaaggatat tacaatttta atatcaaata
     4441 gtggaaattc tatggaagtg gtaaactgta taaaatatat taaagcaata ggttctaaga
     4501 caattgcatt tacttcaaac agaaattcag tactggcaaa agagtgtgat tatgctttaa
     4561 tatatcctgc aaaggatgaa gcagaccatt taaatctagc accaacaaca tcatctacaa
     4621 ttactttggt tttaggagat tctattgcat gtgcattatc taagagtagt aattttggtt
     4681 cttcagattt ttataagtac catccaggag gaagtttagg tgagaagtta aaaacagcta
     4741 acaatggata aaagcaaaaa ggagagtagt ttttatgttc aaaatgttta aaaaaaaatc
     4801 aataaaatca cccatatcag gaaaagtaat agagttatct aaaatacaag atggtgtatt
     4861 ttctcaaaaa attatgggag agggtgtagc tatagattct acaggagaca ttgtatatgc
     4921 tccaatcaat ggaaagatag tagtagtagc agagactaaa catgcatttg taatagaatt
     4981 agaaaatgga atggaacttc ttattcatgt tggtttagat actgtaaatt taaaaggcga
     5041 aggttttgag gcattggtga gtatgggaga acaagtgaag gaaggaacac caatgttgaa
     5101 aattaaccgt cctttgattg aatcaaaggg tatatcactt attactccag ttacaataac
     5161 taatcattca gagtacaata tgaatacatg taatgtaggc aatagtgttg aaggtgggaa
     5221 agatactgta attgagttta aataaagaac taaaatacaa ggttaatatt tataaatacc
     5281 tataaactta tgttttataa gattgattgt atagatttgt tgtatagatt tgttgtataa
     5341 aaaagaactc tttaattata aattaaggag ttctttttta ttttgtaaat attgtttaga
     5401 tttatataga aaaacactta gtaataaata gaataaataa gtatttatat agctaaaaat
     5461 ctaaatagtt ttgtttaaat atatttttat attttgtaat ttcaaatta
//
LOCUS       DAEORZ010000200         5497 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEORZ010000200 DAEORZ010000000
VERSION     DAEORZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.204"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            49..2280
                     /gene="pflB"
                     /locus_tag="KRH39_003531"
     CDS             49..2280
                     /gene="pflB"
                     /locus_tag="KRH39_003531"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBG8513401.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPGALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            2440..3174
                     /gene="pflA"
                     /locus_tag="KRH39_003532"
     CDS             2440..3174
                     /gene="pflA"
                     /locus_tag="KRH39_003532"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBG8513402.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3296..4966)
                     /locus_tag="KRH39_003533"
     CDS             complement(3296..4966)
                     /locus_tag="KRH39_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBG8513403.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINMNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
BASE COUNT         1945 a          796 c          796 g         1960 t
ORIGIN      
        1 aattttaata gtattattta tcatttaaat ctaaggagga aatacttaat gaatgcatgg
       61 caaggattta aaacaggaag atggactaaa gaaataaatg ttagagagtt tatacaatta
      121 aactattctc catatgaggg aaatgactct ttcttagctg gtgccactga aaatactaaa
      181 aaattatggg acgaagcaat ggtcctattt aaaaaagaaa gagaaaatgg tggtacttta
      241 gatgttgata caaaaactgt ttctggaata gctgcatatg ctccaggata tttagataaa
      301 gaattagaaa caatagttgg gttacaaacg gatgcccctt taaaaagagc tgtcatgcca
      361 tatggtggta taaagatggt tgaaaattca tgcgaagctt ttggatatga attagaccca
      421 gaaataaaag atatatttac taaatacaga aaaacacaca accaaggagt ttttgatgtt
      481 tatactccag aaatgagagc tgctagaaaa tctggtataa taacaggtct tccagatgct
      541 tatggtagag gtagaataat tggtgactac agaagagttg ctctatacgg tgttgatgct
      601 ttaatagaag ataaaaatga gcaaaagaaa tcattagaag tttcttgcat ggatgaggaa
      661 gttatcagat taagagaaga aataactgag caaatatcag ctttaaatga acttaaaaaa
      721 atggctgaat cttatggatt tgatatctca aaaccagcaa ctaactcaaa agaagctgta
      781 caatggttat actttggtta tttaggtgct gttaaagacc aaaatggtgc tgcaatgtct
      841 ttaggtagaa catctacttt cttagatata tactttgaaa gagatttaaa agctggaatc
      901 gtaacagaag aagaattaca agagtatatg gatcattttg ttatgaaatt aagaatggtt
      961 aagttcctaa gaactcctga ttacaataac ctattctctg gagacccaac ttgggtaact
     1021 gagtgtatag gaggtatggg aatagatggt agaacattag ttactaaaaa ctcatttaga
     1081 atgttaaata ctctatatac attaggacct tctccagaac caaacttaac agttctatgg
     1141 tcaactaaat tacctcaagg attcaaagat ttctgttcta aagtatctat agatacaagt
     1201 tcagtacagt atgaaaatga tgatttaatg agagcatact ggggagatga ctatggtata
     1261 gcttgttgtg tatctgcaat gagaattggt aaacaaatgc agttctttgg agctagagtt
     1321 aacttagcta aaactttatt atacgctata aatggtggtg tagatgagaa atctggtgtt
     1381 caagttggac caagatttga acctataact tctgagtact tagattatga tgaagttatg
     1441 agtaaatttg aaccattcac tgattggtta gcaacattat atgtaaatac tttaaatgta
     1501 atacattata tgcatgataa atattcttat gaagcattag aaatggcatt acatgataga
     1561 gatatattta gaactatggc ttgtggtatg gctggattat cagtttgtgc agactcatta
     1621 tctgctataa aacatgctaa ggttaaaact attagaaatg aacaaggaat agctgttgat
     1681 tttgaaatag aaggagatta tccaaaatac ggaaataatg atgatagagt tgatagtata
     1741 gctgtagagt tagttgaaag ctttatgaat aaaataagaa agaataaaac ttatagaaat
     1801 tcttatccaa ctcaatctat acttacaata acttcaaatg ttgtttatgg taagaaaact
     1861 ggtaatacac cagatggtag aagagctggt gctccatttg ctcctggtgc taacccaatg
     1921 catggtagag atactaatgg tgctttagct tcattatctt cagttgctaa attaccatat
     1981 gaacatgctc aagatggtat atctaatact ttctctatag tacctggtgc tttaggaaaa
     2041 gatatgactg aaagaataaa caatctttct gcaatgatgg atggatactt cgctcaaaat
     2101 gctcatcact taaatgtaaa tgtatttgat agagctactt tagaagatgc tatggaacat
     2161 ccagaagaat atccacaatt aactataaga gtatctggat atgcagttaa cttcataaaa
     2221 ttaactaaag agcaacaatt agatgttata aacagaacat tccacggtaa aatggcttag
     2281 tctttaaatt tttatacttt aactaagatt actactgtgt aaaaaataaa aataaaaggc
     2341 tatcttatat ttgaattatt tctggtataa gatggccttt tttagagaaa tattataact
     2401 attgataagg ttattaaaat atattgtgag gtgaactcta tgattaaggg aaagatacat
     2461 tctatagaaa cgtttggtac tgtagatgga ccaggaataa gatatatatt attttttcag
     2521 gggtgtcctc ttagatgcaa atactgtcat aacagggata catgggatat taaatcagga
     2581 aaagaataca cagttgatga aataataacg gatgctctta agtatacttc ttttatgaag
     2641 ttttctggtg gaggtataac tgcctctggt ggtgaatcca ctcttcaacc agaattttta
     2701 agtgaattat ttaaaaaagc aaaagaaaat gatatacaca cttgcttgga tacatctgga
     2761 tttgtagata tagaaactat agacccagtt ttagataata ctgatttggt tttacttgat
     2821 ttaaaacata tggttgagga aaaatctatt gatttgactg gtgttggtat ggacaaggca
     2881 ttaaaacttg ctagacactt agaatcaaga aatattcctg tatggataag acatgtttta
     2941 gttcctggca ttactgatga tatagataat ttagaaaaac taggtcaatt tgtagctaca
     3001 ttgaaaaatg tagaaagatt tgaattgcta ccataccatt ctatgggaat acacaaatgg
     3061 gaaagtttag gtatcgatta tgaactaaaa gatgttcctg atgccactaa ggaagatgtc
     3121 caaaaagcta gtgaaataat ctctaaattt ggagtgaaag tatataatag ttagtgtagt
     3181 atattaatcc tttatagaag acataacaaa atagggtaaa gtgtaaattt ttaataaact
     3241 tcaaaacttc cactttgccc tatttttata tactatatat ctcattttat atacatcact
     3301 ttgatagctg aaatttataa aattcagagg cacttacagg tcttccaaat aaaaaaccct
     3361 gaataatatc tacacctaag ctttttacta tatcatactc ttcataagac tctattccct
     3421 caacacaaac ttttaaattt ataatatgag ctaatttaat aatatattca agaaaagtat
     3481 attcataact attatatgta atattcttaa caaaacttct ttcaattttt attatattaa
     3541 cagacagttc tttaaggtaa tttaaagatg aatatcctgt accaaagtca tctattgcaa
     3601 tatatactcc tatacccttt aaactaataa atttatcatt tagtaaattt atatctggta
     3661 tccaacaatt ctctgttaat tccaaaatta aaaattcagg tcttaattga tactctacta
     3721 aacattctac tataaaatct ctaaaaaaat cttcctttaa ctgaatatac gatacattta
     3781 cagatatttt aaagtctgga tttattttat gccattcttt acactgtttt acagcttctt
     3841 ttataatcca cttcccaact ggtataatta aattactctg ttctagtatt ggtataaatt
     3901 caacaggaga tacttctcca taagtactcg aatgccatct taaaagtact tctgccccta
     3961 ttacctcttt tgtaacagca tttacctggg gttgaaaaaa cagctcaaag tcattaaaat
     4021 tattttcaac gcactctctt agtttttgtt gcattgaaat tacctttagt ttattttcat
     4081 agagctcttg tgaaaaaaac ttaattctat ttttaccact aatttttgct atatccaatg
     4141 caatatcagc atgtttaaat aaatctaaat aattatctcc atcttctgga tacatagcaa
     4201 ctcctgcagt cactgtgcaa tagtacttat ttgattcaat ttcatgctga gtatttgtat
     4261 acatctgtat cttttcatat attttctcta tagtttcttt agtacacata gggtaaaaaa
     4321 atgcaaattc atctccatct aacctataca atcttacatc ttttggaaga tattctaata
     4381 cttcagtagc aattgcacgc aatactttgt caccataaga atgtccatat ttttcattta
     4441 tatttttaaa attatcaata tccataataa acatagcacc atttttataa attttctctt
     4501 ttataagata attcattcta tgttcaaatt gttctctatt ccacgttcct gtgatagaat
     4561 caaatttatt tttttcacca atattcttta ttctcccaac tagaaatata gtttttgggt
     4621 catccgacac ataaacttta cctctacaag aaatccatac tatttcttca tatttattaa
     4681 tcaacctata ttctaaattg tgctcacctt tttttccatg tagtaattct tgtatatcat
     4741 ctttccatat ttgaacatca tccggataaa caattttact ccaacaattg accaaatcag
     4801 attctatctc tttacttaag ttaaattcat caaaaattgc tgaagaaatt ttaaatttat
     4861 tcatatttat atcccaaaaa aatatataat cttctgtact ttgagataaa agttcaaaga
     4921 aaatttctat atggtctaaa cttaaagagt tcgattcttc tctcatcttt tccccctaca
     4981 atattactat tagtgtagtt taatcaacaa aatatcctta tttagattat actattttac
     5041 taatttttta tctcatcaaa cacatttccc caactttagt tatcttatca aatttcttac
     5101 tcttaactaa gttttttgag attcataaag taatttttct ataatcatta ctggtgattt
     5161 atttttacct gctaaaacac catgttaaat catttattaa atcattttct cttaaaattc
     5221 tatttttcat tatataaaaa gttctcagtt ctttatccta taaaactctt ctattactct
     5281 tgaatttatt ttattttttt ctccgcctat tctctttgga tttggtattg cttttctata
     5341 gattttggca aagaagtacc tcttactata atataagatt ttgatatttc ttcatcatct
     5401 ataaattttt taatagggat tatatcattt tttatattta tttatataaa tattttttgt
     5461 ttttaatttt ttctttcttc ttatgtactt acattta
//
LOCUS       DAEORZ010000201         5446 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEORZ010000201 DAEORZ010000000
VERSION     DAEORZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.205"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(186..869)
                     /locus_tag="KRH39_003534"
     CDS             complement(186..869)
                     /locus_tag="KRH39_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBG8513404.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(1045..1635)
                     /gene="zapA"
                     /locus_tag="KRH39_003535"
     CDS             complement(1045..1635)
                     /gene="zapA"
                     /locus_tag="KRH39_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBG8513405.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(1660..4053)
                     /locus_tag="KRH39_003536"
     CDS             complement(1660..4053)
                     /locus_tag="KRH39_003536"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBG8513406.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGIKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(4070..5089)
                     /gene="pheS"
                     /locus_tag="KRH39_003537"
     CDS             complement(4070..5089)
                     /gene="pheS"
                     /locus_tag="KRH39_003537"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBG8513407.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(5139..5373)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1654 a         1076 c          596 g         2120 t
ORIGIN      
        1 tatatattgc ctactatact taagacattt taattaattt atacactgct tactatactt
       61 aaaatacttc tattaactta tacattgctt actatatttt aaattcttta attaatctat
      121 acaatatttg ctatatataa agtatatcaa ataacagctt catttttttg taaatttaca
      181 gaaagttaat tttcattaaa tgaatcatct ttattgataa acataattcc aaagaccact
      241 gccaaaataa aaaaacatat tagtattgat ttttccacga aaatttttgt aaaaaaagtt
      301 cctaaaatag ctccagatat aaagaatata ataatagcat aatactgtaa actctttttt
      361 atcataaaaa tattcttagt atgtatataa tgaaataaag tttctgtagc acttcttaaa
      421 tttccagtac acattgtcgt tgcaaaacta ttcccattta cttttctaaa actctctact
      481 tgcattgaac aaataaaaga aatagcaaca tttacaaaca tattaaatct tcctctagga
      541 ataaatgaaa caataagtat tgtaatcatt tcaattataa tgataacctg acgccaatga
      601 atctttgtct tattatttgt aaaatacttt cttatcttct cggctataaa tacacctaac
      661 ataaatgcca aaataggaat caaataataa aatgattcct ttacattacc ttctgctata
      721 ttaagcccaa acaaaactat atttccagtt tgtgcattag caaagacttt acctcttacc
      781 acataagtat aaatatccaa aaatcctcct gttattgcca acaaagaaca taaaaagaat
      841 gattcagaca tctgactctt gcttgtcata tataaacctc tcgctttcta atgatagata
      901 caaaatcaaa atcattttag attctatttt tcattgatat aaaaaaatta tttatattta
      961 aatattgtct atatttatac atcatttata tttatacatt aaaagaagct gtctcaaaaa
     1021 acaagagaca gcaaatcttt tagtttattc cacatttttt gcatcattat ttaaccctgt
     1081 acattttaac tgtaaatcat aagctttgtt ttgaaactca gaagctaagt tttcagctat
     1141 agtagctctt tcatttgctt cttcaagttg agtacttaac tcctcaatct tgtttttata
     1201 agcatctaac tctgctctag aaccttctgt cgtattacta agttcgaata tttcttgctt
     1261 tttattttct ataatctcaa ttaactcttt catcttagtt tcagcttcac gattttctgc
     1321 ttctttttct gctatcgtaa gctttaaaga cttgatttct aattgaagct cttcattaga
     1381 tgctccaacc ttcttactca attcttcatt agctttaatc aacttctcat tctcatctga
     1441 acattcaaat aaaaggtctg atatatttaa tgcagttaat atcgcagcaa ctgataagct
     1501 caatttcggg ttttgacgtg ttattttatc catttctttg tctacaaaat cagcaatact
     1561 aatcataaac ttctctgatt tgtctccaac cattggatat tcagcgccat ggattttaac
     1621 cattactttg ttcatcctaa cctcccccga cattagttgt taatttgatc ttaagttagc
     1681 atttaatttt tcgcttaatt cacttaatat cttatcatgc accttagcta catcttcatc
     1741 tgttagagtt ttatctttac ttctataagt tattgaatat gcaattgact tgtatccagc
     1801 ttctatttga gaacctttgt aaacatcaaa taacttgtag ctttctacta atccttgacc
     1861 attttccttt attatatctt ctatttgttt tacaaataca tcatctttaa caataagagc
     1921 tatatctctt gatgtagatg gatattttgg taatggcaca taattctttg ttctatcaga
     1981 attatcaaat acaaagtcaa tatctatttc agcgacataa actctttgac ctagattgta
     2041 gttttctata acatctggat gcaattcacc taatgtacct acatatttat tattatacac
     2101 tattttagca catctaccag ggtggaaggt agtattatta tcttgaggtt caatttcata
     2161 acctttaaat ccgacattta caagtatagt ttctatagtt ccttttagtg caaagaagtc
     2221 aacatcttta ccatacatac ctacacacat tctattttct tccttaggta attctgaatc
     2281 ttgtgggatg aatatatgtc cacactcaaa tgcagatact tcttctattt tatgagatac
     2341 attagttgat aaaacatcta acatatttgg tattaaagta gttctcatta cagaagtttc
     2401 ttctcctaat gggttaagta atttaacaaa atttctcttt gcattacctt ctggtactct
     2461 tatcttatct actcccttag ggcttacaaa tgaatatgtt aatatttcat ttaatcctac
     2521 agcagttgaa ttatctttta aagcatccat gaatttttgt tttgaagttt taattcctgc
     2581 tgtcgtattt ccttctaatt gtacagaagg tatattctca aatccataaa ttctagctat
     2641 ttcttcccat acatcagctt cttgttccat atctgttcta aagcttggta catctatttc
     2701 taatttatca ttagctacca aattacattt aaactctaat gattctaaaa ttcctacaaa
     2761 ctgctccatt ggtacatcta cacctaatag atggttaatt ctttgaggat ttactaccaa
     2821 ttttttaggt tctggtttat ttggatatac atctacaaca ccttttaaaa cttttcctgc
     2881 tcctaacatt tcaacaagct gtgcagctct atttgctgct atctctgcaa gattagggtc
     2941 taagtctttt tcatttcttg aagatgcttc tgacctaata ccaacttttt tagctgtcat
     3001 tcttatgttt tctggtttga aattggcact ttcaaaaagt acagtcttcg tattagaagt
     3061 tatttctgag ttagcaccac ccattacacc agctaaacca agtgattttt ctccatttgt
     3121 tataactagc atatctttgt ctaatgttct ctcaacatca tctaatgtta caagtttctc
     3181 tccatcttta gcatttctta ctactattct tccagtctct acttgattta tatcaaaagc
     3241 atgaagtggt tgaccaagct ctaacattac gaagtttgtt atatcgacta tgttacttat
     3301 aggtcttact cctgcttctg taagtcttct ttgcatccaa tatggagaag gttctatttt
     3361 tacatctgta accattctag caacatatct tctacataaa tctggattat ctatctcaac
     3421 tttgaaatct atctcttcgt cactttcttt tacttcgatt tcaggatatt ttacttttgt
     3481 tcctatagtt gcagctgctt ctctagctat acccatcatg catttacaat caggtctgtt
     3541 tgaagttaat tcaaaatcta ttaaagcatc ttttaatcct aaaacatctt ttatatcttt
     3601 tcctaattca taagaatctt cgtggtctaa aatatatata ccacctcttt tatactcctc
     3661 aatgtaatgt tcatctatac ctagttcagc tgctgaacac atcataccat ctgataattc
     3721 acctctgaaa tcagtctgtt tgatttcaac tcctccaggt aacttagaac catttacagc
     3781 tactggaata taatctcctt ctgatatatt tgtagctcct gtaactattt gaacaacttt
     3841 atctccaata tctactttag ttacaaccaa cttatcagca tttggatgtt gttttatttc
     3901 taaaatcttt ccaaccaata tattttctat ttcttcacca taataatcta ttgtttcaac
     3961 tttaattcct gtcattgtca ttttatcagc gaactctttt acatccatgt ctatatcaac
     4021 atagtctcta agccatttta aagatactaa catctacata ccctcctaat taaaattgat
     4081 ttaagaatct catatcattt tcgaataata atctaatatc atctatttcg tatttaagca
     4141 ttgcaagtct ttcaacccca actccaaatg caaatccact gtaaacttct gggtctattc
     4201 cacaatttct aagcacattt ggatgaacca tacctgaacc taatatttct atccaacctt
     4261 catatttaca cattgggcaa cctttaccac cacatttgaa acaagtaaca tcaacctctg
     4321 cacttggttc tgtaaatggg aagttgtgag gtctaaactt agttttgata tcagaaccaa
     4381 acaatttttc aacgaagata tccatagttc ctttaaattc tgccatagta acatcttttc
     4441 caacaacaag cccttctatc tgatggaaca ttggtgagtg tgtagcatct ggcgagtctg
     4501 acctaaaaca tctacctggt gcaattactt ttattggtaa ctcttgactt ctcatagttc
     4561 ttacttgaac tggagatgtt tgagttctaa gtaatacccc atcattgata tagaatgtat
     4621 cgctcatatc tcttgatgga tggtctttag gagcgtttaa tgcgtcaaag ttgttttcaa
     4681 cagtctcaac ttctggccct tctgctatag agaatcccat tccgataaat atatctgtta
     4741 cttcatctat aatttgagtt attggatgct tcttcccaac ctttaaaact tttcctggtt
     4801 gagtaacatc tatcacttcc tcttttaatt ttctttcttt ttcaatagca tttatttctt
     4861 cttttttaga atttatgcta agttcaatgt tttctcttac ctcattggca accttaccaa
     4921 ctactggtct ttcttcagca gataatttac ccatttcttt aagtatggca gttatctcac
     4981 ctttttttcc taagtactta actcttaaac tttctacact ttctatgctt tgtgcttctt
     5041 ttatttcagc caaagctgct tcacgtaaag caagtaattt ttcttgcaca catatcaccc
     5101 tttcgttttt ttgtataaaa aaagcccctc aatccctgtc tagggacgaa aggctatagt
     5161 ttcgcggtac caccctagta gtttaacaat ttaaaaatca tcaaaccacc tctaacaaac
     5221 ttaacgcgtt tttgacgtca aagcctactg taaaatttca gctctgttac tccagaggga
     5281 acttctcatg acctcttaga aaacactccc agcctaggtg tttatctctt tactaagttt
     5341 aatcaaaata ctttcctctt tcattgcatt tacaatatta tattgtttat tttagtatta
     5401 tatcatttcc attcaaatta tgtgactatt tttaatatat atccaa
//
LOCUS       DAEORZ010000202         5442 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEORZ010000202 DAEORZ010000000
VERSION     DAEORZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.206"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            142..522
                     /locus_tag="KRH39_003538"
     CDS             142..522
                     /locus_tag="KRH39_003538"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBG8513408.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRDEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            537..998
                     /locus_tag="KRH39_003539"
     CDS             537..998
                     /locus_tag="KRH39_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG8513409.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            1019..1609
                     /locus_tag="KRH39_003540"
     CDS             1019..1609
                     /locus_tag="KRH39_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513410.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            1624..2781
                     /gene="alr"
                     /locus_tag="KRH39_003541"
     CDS             1624..2781
                     /gene="alr"
                     /locus_tag="KRH39_003541"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG8513411.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            2814..3101
                     /locus_tag="KRH39_003542"
     CDS             2814..3101
                     /locus_tag="KRH39_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBG8513412.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            3070..3453
                     /locus_tag="KRH39_003543"
     CDS             3070..3453
                     /locus_tag="KRH39_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBG8513413.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            3624..4451
                     /locus_tag="KRH39_003544"
     CDS             3624..4451
                     /locus_tag="KRH39_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG8513414.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            4444..5385
                     /locus_tag="KRH39_003545"
     CDS             4444..5385
                     /locus_tag="KRH39_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG8513415.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIVKTVKEAIIAKRM"
BASE COUNT         2171 a          570 c         1087 g         1614 t
ORIGIN      
        1 ttaaaagtta cagttttatt ataatgatta ttaaattaag ataagtaaca taaactaaat
       61 aataatgtgt attatcgata gtaaatttat aaattcatta ttaattattg aaagaagtat
      121 tataaatttt ctggaggaac aatgaatata tttgatattg gcgtagatat aattgaaatt
      181 gatagaataa gaaaggctgt agacaaaaat aataggtttt tggagaagat atttacagat
      241 agagaaatag agtattttaa ttctaagaat tttaaagcag aaagtatagc aggtaatttc
      301 gcagccaagg aagctataag taaatctata ggtacaggaa ttagagtgtt taattttaaa
      361 gatatagaag ttctcagaga tgaaatggga aaaccaatag taaaaactta taataatcta
      421 gcaaaaatgt gcatagatta taatgtttta gaaataaaag tttcaatatc acattcaaaa
      481 gattatgcta tagcaaatgc gataaccata attaaggact aaggagtgat tacattatga
      541 tggataaaac agcaaaagaa ataatgacaa cagatgtaat agtagcaaaa caagatgata
      601 gtatagcaga tgtagctaat atgctaattg cagaaaaaat aggaggtctg ccagttgtag
      661 attcagagaa tagagttgtt ggaataattt ctgaaacaga tattcttaaa aaagaaaaat
      721 atatagaagc acctctctat ataaatctat tacaaggatt gattttctta gatgacttaa
      781 aaaaagttga aaaagatata aaacaagtag ctgcttataa ggttggagaa cttatgtcta
      841 aagatataat aaaggttcat gaagatgata agtttgatga tgtagctaat gtaatgatta
      901 aaaaatcaat aaacagagtt cctgtagtag atgatgacaa caaacttaaa ggtataattt
      961 gtagatatga tataataaaa gctttataca atgaataaac tgaagtagga ggtgtgatgt
     1021 gagaaaaaag tggaccatag tatgtattat gttcttggca ctggttataa ttgtaatagg
     1081 atgccaaaaa cgacagtcca caaaagaaga agtatacaaa gattttcaaa agcagatttc
     1141 tgatatgaat tattacagtt gtaaagctga agtagaagta gttggaaata aaagtccaca
     1201 caattatgtt ttaattcata cttataaaaa aactgataac tataaattag aagtaatttc
     1261 cccaaaacat ttaaaaggga aaagtataga atatcaaggt gataaaatat tagttaaaaa
     1321 tccaaagatt agtgatgtag tggagttacc taatacagga aaaaataatc aatatctttt
     1381 tgtaggagac tttataaaaa actatcttca aaatgaggag atgaaagtaa agttatccaa
     1441 aggacatttg gttttagaga cttttattcc tggggataat aaatatttta acaagcaagt
     1501 attatatgta aatgctgaca caaaaaatcc tgaaaaaatg gaagtgttag ataaagaggg
     1561 agtgccaaga tttacagtaa aatacaaaga ttttgaatac agaaactaag ggggactaaa
     1621 gacatgcaaa aaataacagt gcctacatgg gcagagataa atctagataa cttaagattt
     1681 aacttaaata atattaaaaa tttattagaa gaagatatta agatttgtgg agtaataaaa
     1741 gctgatgcat atggacatgg tgcagtagaa gttgcaaaat tgctagaaaa agaaaaagta
     1801 gattacttag cagtagcaag aactgctgaa ggaattgaac ttagacaaaa tggcataaca
     1861 cttcctattt tgaacttggg atatactcca gacgaagctt ttgaagattc tataaaaaat
     1921 aaaataacta tgacagttta ttctttagaa acagcacaaa agataaatga aattgcaaaa
     1981 tctttaggag aaaaagcctg tgttcatgtt aaaatagact cagggatgac tagaataggt
     2041 ttccaaccta atgaggagtc agtacaggaa ataatagaat taaataaatt agaatatata
     2101 gatttagaag gtatgtttac tcattttgct acagctgatg aagtaagtaa agagtacact
     2161 tataaacaag ctaataatta taaatttatg tctgataaat tagatgaggc tggtgtaaaa
     2221 atagctataa aacatgtatc aaacagtgca gctattatgg attgccctga tttaagatta
     2281 aatatggtaa gagcaggaat aatattatat ggtcattatc catctgatga tgtatttaaa
     2341 gatagattag aattaagacc agccatgaaa ttaaaatcaa aaatcggaca tataaaacaa
     2401 gttgaaccag gtgtaggaat aagttatgga ctaaaataca caactacagg taaagaaaca
     2461 atagctacag ttccaatagg atacgcagat ggatttacta gaatccaaaa aaatccaaag
     2521 gttcttatta agggagaagt gtttgatgta gttggtagaa tatgtatgga tcaaataatg
     2581 gttagaattg acaaagatat agacataaaa gttggagatg aggttatact atttggagaa
     2641 ggcgaagtta cagctgagcg tatagctaaa gacttaggaa ctataaacta tgaagtgtta
     2701 tgtatgatat caagaagagt tgaccgtgtt tatatggaaa ataatgagct tgtacaaata
     2761 aacagttatt tgctaaaata aaaccatgat attataatct gggaggcgat tttgtgtacg
     2821 ataagagtaa agaaaaaata gaagttactt tacctgactc tctgatttct gaagttgata
     2881 gtatagttca gatggagaac agtaacagag aagattttgc aaaagctgct tttcagttct
     2941 atataactca gaaaaagaaa attcatatta aagaatctat gaaaacgggt tatagagaaa
     3001 tgggtcaaat taatctgtca ctagctgaat taggtatgac agttgaagaa gtatcttctg
     3061 atgataaaaa tgaagggaag attgcccaag gtgagtacta attgtgaaat aaagcgtggt
     3121 gatttatatt acgctgattt aagtccagtt gttggttcag agcagggagg cgttagacct
     3181 gttctgataa tacaaaatga tgtaggtaat aaatatagtc ctacagttat agtatcagct
     3241 ataacatctc agattaataa agccaaatta cctactcatg tagagataag ttccaatgaa
     3301 tatgggctta ataaagactc agtaattctt ttagagcaaa ttagaactat cgataaaaag
     3361 agactgaggg aaaaaattgg ttgtctagat gaaaatatga tgataaaagt agatgatgga
     3421 cttcaaataa gtttaggatt gtttactttt taaagaggga tttgtctttt attagacaat
     3481 cccttttttc ttttaaatat ctcttaaggt ttaattttaa tgtactatgt taataaaatt
     3541 aaaaataacg caaaaaaata gcataaaagt gatataataa tatggaattg atataaaatt
     3601 acgaactcaa gggaggtaat tgcatgagag accataaagg attaaatgag attgcgcgta
     3661 tcataagaag agatatagtt tcaatgatac atagagccaa atctggacat ccaggagggt
     3721 cattatcagt agttgaaata cttactgcgt tatactttga tgaaatgaat gtggattctt
     3781 caaatcctaa aatggaggat agagatagat ttgtattatc aaaaggacat gctgcaccag
     3841 cattatatgc tacattggct gaaaaaggct attttgataa ggaagaactg aatggattaa
     3901 ggaagatagg aaggatgtta caaggtcatc cagacatgaa aggcacgcca ggtgtggaaa
     3961 tatctactgg ttctttaggt caaggttttt cagttgcatg tggtatggct atggcatcaa
     4021 agttggataa tgctccgtgg aatgtgtata ctttgcttgg tgatggtgag gttcaagaag
     4081 gaattgtttg ggaagctgct atgagtgctg cacattataa acttgataat ttaattgctt
     4141 ttttagataa taatggactt caaatagatg gagatataga aagcgtaatg agtttaggtt
     4201 caatagttga taaatttaaa gcttttggtt ggaatgtaat tgagatagat ggacatgatt
     4261 ttgaccaaat atttgcggct cttgatatag ctaaatcaac agttaaaaaa cctacaatga
     4321 tagttgcaaa aactataaaa ggcaaaggaa tatcttttat ggaaaatcaa gcaggatggc
     4381 atgggacagc tccaagtgat gaggagctag aaaaagcctt gcttgaatta ggaggtgctg
     4441 acaatgagta aaatggcgac gagagaagca tatggtaaag cattagtaaa attaggtcaa
     4501 ataaatgaca atgttgtagt attagatgca gatttatcaa agtctacaaa aacacatgat
     4561 ttttacaagt catttccaga tagatttttt aatatgggta tagctgaaca aaatttaata
     4621 ggcgctgctt gtggattatc aactgctggt aaaatacctt ttgcaagtac ttttgctatg
     4681 tttgctacgg gaagggcttt tgagattata agaaattcag tttgttatcc aaagctaaat
     4741 gtaaaaatat gtgctactca tgcaggtctt actgttggag aggatggagc atctcatgaa
     4801 agtgtggaag atattgctat aatgagagct ataccaaata tgacagttct tgttccagct
     4861 gatggagttg aaacagaaaa aattattttt gaaatagcta agtacaatgg acctgtttat
     4921 gttagattag ggagaagttc agtaccagtt ttatttgatg aagattataa atttgaaata
     4981 ggaaaaggta cagtcttaag agaaggaaag gatgtatcaa ttatagcttg tggaattatg
     5041 gtaaatgaag ctctattagc acaagaaaag ttgcaagaag aaggtataag tgctagagtt
     5101 ataaacatgt cctcgataaa acctatagac aaagatttaa tattagaatc agcaaaagaa
     5161 acgaatgcta ttgtaactgt agaagaacat agcataattg ggggattagg ttctgctgta
     5221 agtgaggtag taggagagtc ttgtccaacc attgtaaaga aagttggaat taaagataca
     5281 tttggagaat ctggtactcc aaatgaattg ttaaaaaaat atgaattaac ttgtgatgac
     5341 atagtaaaga ctgttaaaga agctattatt gcaaaaagaa tgtaagagat ataaattatt
     5401 catgtttgtt aataatataa tatatttaaa agagaggtat aa
//
LOCUS       DAEORZ010000203         5391 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEORZ010000203 DAEORZ010000000
VERSION     DAEORZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.207"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(527..1933)
                     /locus_tag="KRH39_003546"
     CDS             complement(527..1933)
                     /locus_tag="KRH39_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8513416.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLVLKDGKFILDDSSKEILHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKGQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(1978..2685)
                     /locus_tag="KRH39_003547"
     CDS             complement(1978..2685)
                     /locus_tag="KRH39_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8513417.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKEGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2856..3770
                     /locus_tag="KRH39_003548"
     CDS             2856..3770
                     /locus_tag="KRH39_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513418.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3770..4531
                     /locus_tag="KRH39_003549"
     CDS             3770..4531
                     /locus_tag="KRH39_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513419.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGVLIYYIGRNHLLKKEV"
     gene            4543..5280
                     /locus_tag="KRH39_003550"
     CDS             4543..5280
                     /locus_tag="KRH39_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513420.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1864 a          834 c          699 g         1994 t
ORIGIN      
        1 aaaatactac acaaaaactc tattttagta gaactcaatg gcgcaaaata cttcaattta
       61 aaacatttat cgtactttaa ataaataact ataataaaat atacttacaa tagttacata
      121 gaagctatta taactacaca gaacagtgta taattaaaac ttttttagga tgtcattata
      181 ttttttctta aaattaaaaa acactatacc ataattctat tttagtagaa tttttttgtc
      241 aataataaaa ttaaatatta actaaaaaat aaaaaaaatg gaattatcta aagaatttaa
      301 taaccttaca ataattccaa aatattattt ctatgaaaat tttgatacca tgagtaactc
      361 agccataagc gcataaagca caagttactc tttactttaa taaaacttga caagaaaaga
      421 atttatcctt attctactct aatagagtta atttgtcaat aaagaataaa attattttca
      481 aaatattttc attcttcatt cttatctgtt attattttat aaaatatcac ttaattagag
      541 gtaattttag tattgttttt agtccctgac tatcaatgct cgtaatatct aatttacctc
      601 tatgtgcatt aataatgcca gatactatca aaatacctag accatgattt tgacctttgg
      661 catatgcctc acttagtcca ttttggccct ttaataaact attaattctt tcttgactca
      721 taccttttcc atcatcttca acaattatat agcaaaaatc taaatcagac ttagaggtaa
      781 cccttatatt acatccttgt tggttatgag ttatactatt ttgtattaaa ttactaatag
      841 ccctttttaa taatttttta tctgcatcta taataatatc ttcatttagt atatcaagtt
      901 caacgtaaaa gtcattatcc aaattattat ttataaactc agaaataagc tctctcaaaa
      961 acgcagaaac tctaatctta tcacaattca atgcttgcat attatattca agtttagaca
     1021 ctaaatttag gtcatttact aattctctga ttttaatccc ttgattgcat ataatagaca
     1081 cttgctcttg ttctctattt gaaaggctac tgctttcttc aagttctcca gcatatccaa
     1141 ttatcattga aagtggagtt cttatatcat gagatattcc tgcaatccag ttagacctag
     1201 cttcctcttt cttcctaagt ttttcatctt tcatttgcaa ctctttagat acagtattaa
     1261 tagattttga aatatcactt agtattcctt tttcttgtaa aagtactggt tctccttttg
     1321 acataagctt tatgccagta ataattggtt ttatagattt caccattttt aaacctatga
     1381 atagagctat taaaaaagta actattgcat taaccaaaaa catacctatt attaacatag
     1441 gaaattgttc tatttcctta accgtataag taaggttaaa cttagtgtac ttgtcttttg
     1501 gataacctgt tactaatatt ccatttggat gttcccatac atacacagga tagtccttta
     1561 aaaagaacct tgaaaacttt gctacatctg tcaaagtata atgacttgga atttcttttg
     1621 gtaaatcaaa tccccatatt ctatctccat tgttatcaat taacattgcc caaactttat
     1681 ttttatgtag tatttcctta ctagaatcat ctaaaataaa ctttccatct tttaaaacca
     1741 aattatcttc aatcataatc aagtttgatt ttatttctct gctatcactt gtatctaaat
     1801 tatcagagta tttagataca aataacatat atgaaaacag aagaaaacct gctatattta
     1861 atacaataaa aagtaatgaa atcaagatac ttgaacctat aaatcgcctc aatatgtact
     1921 ttacactatc caaaattaaa tcactcctct tgatttcttt tattctaata tctaaaatta
     1981 attaatcatc aacttatatc caagacctct tactgtaata agatgtttag gtgtagatgg
     2041 agttttttct atcttctctc taacccttct aacatgaacc ataagggtat tttcatatcc
     2101 atacatatca tctccccaaa tggcttgaca tagtgaatca ttagtaacaa ttctcccttt
     2161 attttcatac aactttaaaa gcaacgaatg ctctttagct gttaaagaaa tctcttgccc
     2221 ttctttctca acacatgcac tatttaaatt cacaacaaca gaatctccca gtttaaatac
     2281 tggcaactcc tcttcttcta taggcacata tacacgattt aatataccca caagccttaa
     2341 agtaagttcc tttggtaaaa atggctttac tatataatcg tcagccccta agcccaaacc
     2401 aatcaatcta tcttcatctt cacctcttgc agagagaaaa atcactggta ttttagatgt
     2461 ctctctaatt tttctcataa tagaaaaacc atctccatct ggcaatacaa catctaaaat
     2521 tgcacaatct ggctttacat ttctaaatac cctaacagct tcctcataac ttgatgctaa
     2581 aaatactcta ctaaacccct cttttctcag gatttcatct atcattttta ataattcctt
     2641 gtggtcatca accacaagta tttttttatc ttttattgac tccataatca tcaccctcct
     2701 ttagtatacc atatacccat tttcacaact ttgcctaaat agacttttaa ggttattgta
     2761 aggtaaattt cagctaattg aaatgtaacc aagttattat actgatataa gttaaaagaa
     2821 tcaataagca cagattaaaa aaggggatga aacatatgga aatgataatt agaacacaaa
     2881 gactttcaaa ggaatacaat aaaatattta gagtaaagga tatagaccta agagttccac
     2941 aaggagctgt atatggtttt ttgggtccta atggagctgg aaaatctact actcttaaaa
     3001 tgcttcttgg acttgctaaa cccacacaag gaaatattaa tattttaggt aaagagttaa
     3061 atgaaaaaaa tagaatatca attttaaaag atataggttc tttgatagaa tctccatcat
     3121 actatggtca tctgacaggg cttgaaaaca tgactataat gcaaagactc ttagatttgc
     3181 ctaaaaaaaa tgtaaatgaa gcattaaaaa ttgtaaggtt agaaaatcaa aaaaataaaa
     3241 aagttagtca atattcttta ggtatgaagc aacgtcttgg cattgctatg gcaataatga
     3301 aatttcccaa attattaata cttgatgaac ctacaaacgg actcgaccca gcagggattg
     3361 aggaaattag agaattaata aaatctctac cagaaaaata cggcatgact gttctaatct
     3421 ctagccatct tttaagtgaa attgaccaaa ttgccacatc aataggaata ataaatcatg
     3481 gcgaactggt ttttcaagat agcataaaag aacttcataa tagaggtcaa agtcagattg
     3541 ctatcaaaac taagaatgtt gtatctgcac aaaaactatt aaatactaaa ggatatatgt
     3601 cacttataca agatgggtac ttgactattg aaaatttaaa agactcagat gtggctaaaa
     3661 taaatggtat gcttgtccat tcaaatatag atgtatttag aatagaagaa cataaaaaaa
     3721 gcttggaaga tatattctta gaacttactg gtaaggcggt gactttataa tgaaatacct
     3781 tggtctagaa ttttataaac ttaaaagaaa gaagattatt ctgatgacct ttttatttgt
     3841 agctgttgaa ataatgtggt gtatcgtaaa ttcaaataga gctattacac gtaatccaga
     3901 tatgttaggt ggatttgaat attcctatat gcttatgagt tttgcaagtt taaatggtct
     3961 attctttcct atcttaatat caataattac atcacgtata agcgatatag aacataaagg
     4021 agatacatgg aagcttttaa aatcttctgt gacatcttta aatagtattt atttatctaa
     4081 atttttatgt tctgctattt tggtatctat acctgtattg atgcaagtgt tatctattgt
     4141 gttgtttgga cattttagag gggttattga gtcaatttct gtatctttac ttttaaaata
     4201 cacattagga acaattcttg taagtattgc tatcattgca ctacacctat ggattgcaac
     4261 tgtaattcca aatcaaatga ttgcaattac atttggcatg attggtagtt ttataggttt
     4321 gacatcaggt ttgttttatc aaggtattcg tagattgttt atatggtctt attacctaga
     4381 gttatcacct ttaagttatg cttatgataa tgttttaggt tcatctgtct atagaattga
     4441 tatgaatttt tctattacga ttttagtttt cttaattgga gtgctaattt attatattgg
     4501 aagaaatcat ttattaaaaa aagaagtata ggagggattt aaatgttaag gaagtcagtc
     4561 ttgggagaaa ttctaaagtt aaaaaattct tttatatggt atgtattatt agctttgcca
     4621 cttataagtg tgttgattgg ttctggtaat ttttatctaa atcaaggcat actcaaaaaa
     4681 gaatggtaca gcttatggac tcaagttagt cttttttatg gggaattttt ctttcctatc
     4741 ttaatcgcaa tcttttgtgc atatatttgt aggcttgaac atatgaatca taattggaat
     4801 aatgtaatga cactgccagt taatttaaaa aatatctttt tatctaaact tacagtagtt
     4861 tcaatactta cagctattac acaagttttc tttgttatct tttatatagt tgcaggaaat
     4921 atgtttggtt tttctggtag tgtacctaaa gaattgactg gttggattgc taaaggttgg
     4981 attatttcta tttcaatagc ttctatacaa ttatacatgt ctataaagat tagaagtttt
     5041 gcaactccta ttggtattag tctttgcctt tctctatttg gtatgggatt aatagtagca
     5101 aaacctgctg ttggtatatt tttcccttat tctatgttag gtagtgctat gggggttata
     5161 aagcaatctg ctttaaatgt taatgacact atagttacaa tatgtgttgc atttattact
     5221 actatagtat tttctgaaat tgccaataga aattttagaa agaatgatgt tatttcttaa
     5281 tggtaaatgc tagatatttt ttggtttgaa atttatattc tttttagttg aaatctatat
     5341 ccattttatt taaaaatata tatcttttat ttgaatctta tatgctttat c
//
LOCUS       DAEORZ010000204         5352 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEORZ010000204 DAEORZ010000000
VERSION     DAEORZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.208"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(204..1388)
                     /locus_tag="KRH39_003551"
     CDS             complement(204..1388)
                     /locus_tag="KRH39_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG8513421.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="KRH39_003552"
     CDS             1761..4112
                     /locus_tag="KRH39_003552"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBG8513422.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPKDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="KRH39_003553"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="KRH39_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBG8513423.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2098 a          596 c          849 g         1809 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttacca tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaattta
      241 tctaatccag ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggtt ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtatgggaa ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat ttgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caaggttgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagaacat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcata tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaacac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctaaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actattccct aattttagtt
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgcc ttgaggtaca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta ggaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatat ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gtttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tatcgataca ttatttaatc
     4861 gattcttatg ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat
     4921 ctagataagt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatatta agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac
     5041 taaacacata agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat
     5101 gcaatatcta tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat
     5161 aatgtggata atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg
     5221 gataatatta taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt
     5281 tgttgactct aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca
     5341 aataaagcat aa
//
LOCUS       DAEORZ010000205         5291 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEORZ010000205 DAEORZ010000000
VERSION     DAEORZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.210"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(403..987)
                     /locus_tag="KRH39_003554"
     CDS             complement(403..987)
                     /locus_tag="KRH39_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG8513424.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
     gene            complement(1309..3153)
                     /locus_tag="KRH39_003555"
     CDS             complement(1309..3153)
                     /locus_tag="KRH39_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG8513425.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESVIYWKKNGFISQVSDYLF"
     gene            complement(3448..4410)
                     /locus_tag="KRH39_003556"
     CDS             complement(3448..4410)
                     /locus_tag="KRH39_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBG8513426.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            4601..5152
                     /locus_tag="KRH39_003557"
     CDS             4601..5152
                     /locus_tag="KRH39_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8513427.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
BASE COUNT         1896 a          839 c          528 g         2028 t
ORIGIN      
        1 aaaataaatg ttactcttat taataaaaaa aattataacc acttttgatt actaatcaaa
       61 agaaaatatc taagtaaaat aaaaattata tttgttaagc tttatgtatt atagtatttt
      121 attcttactt aatcatcata ctatagcaaa atactaacct aaaagttagt attttactta
      181 aaaataagtg cttattcaaa catcaaatag aatatatact attataaata aatctataaa
      241 ggaatgactt ttataaaaaa atggtggaaa tgtaagatag aagtgataaa aactacattc
      301 cacaaaaatt taacaaatga atatggatgt gaaagtatta gaaaataccc tatgtataaa
      361 gtaagaaagg tattttttga gattttgcaa gaaaatttaa ctttatttat tttctcgaat
      421 aatacggcat ggatttccgt atgcaatcat attacttgga atatcttttg taacaatact
      481 tcctgcacct ataactacat tatctccaat agtaacacct ggcatgataa tagcaccccc
      541 gccaatccaa acattatctc caatagtaac tggagctgtt tgagttttac aaaattcaaa
      601 tgaaccatct tctttggcat taccaaatct atcaccagca ttagttggat gaaatgcagt
      661 atatatttgt acgtttggtg cgattagtgc attatcccca atgataattt tattatcatc
      721 taaaaacgta caattcatat tgacttcaca attatttcca aaataaatat tatttccata
      781 atctacataa tacggtgcag taatccataa gtttttaccc ttaccaccaa gtaactcatt
      841 taatattttt tctttttttt ccaaatccga ggaatctgtt tggttataat ctcttactag
      901 gtcttttgct ttatgccatt gagttaacaa ctcaatatca ccacaatcgt aaagttctcc
      961 tgccaacatt ttttctcgct ctgtcatgtt tttaatcctc caaatttata ttgtttttca
     1021 tatatattct agcataagat ttattaattt atatatttta cttatataaa atttaataaa
     1081 taacaagatt tattaaatat acagttgaca atctatctct ctgtcatctt aaaactcttt
     1141 taactgtaat aatctcagag caattttttc acttttattc cttgtttata gctgataagc
     1201 acaattttta taagaataac cttaatttaa caaccttatt tatgttaaaa agagatatta
     1261 ttgattttaa taatatctct tttactggat aatacaatta tagaatattt aaaataaata
     1321 atctgacact tgactaataa atccattttt cttccaataa attacactct ctataaggta
     1381 tggattatct gtaataatac tatctacatt taggtcaagc attttctcaa ttaaactgtc
     1441 actattaaca gtccaaacga agattttttt gttcatttta tgaatctttt ctactactgt
     1501 tttattaaca aaagttgatt caattccata tatatcaaca taatttaact ttgaaaaatc
     1561 accatatgca attgccataa aatagcatgt cagtaaattc tcatccagtt tttttacttt
     1621 ttgtaatata gttttatcca tagatgcaac tatacattga ttttcaaaat tattttcttt
     1681 aattaactga acaacttttt ttactacgtc tcccttttca tgtccattaa gttttatttc
     1741 tattaataat ttcaccttac ctttagaata ttttatcatt tctttgagtg ttggtatctt
     1801 ttccccaaca aacttttcat cataataacc ttttccataa taataaaagc ttcccgcatc
     1861 atatgtcttt acttcattat aatttacatc ccaaacattt ttatctactc ctgtagttct
     1921 cttaaagttt gaatcatgaa gtacaataag ttctccatct ttagtttctt gaacatctat
     1981 ttctgcataa tcagctttag catgtgaagc ttcttctatt gctgccaaag tattctctgg
     2041 agccatttca gctcctgctc tatgagcaat tgctgttgta taaaatgaaa atgacgtatt
     2101 aaacatacca tttatatgtg aagaaaatgc aactgactct atataagaaa ttaataatat
     2161 taatactaaa ttaaaaacaa tttttgatat agattttttc tcttttttaa tattattttt
     2221 agaaatattt actcctttat attgataata agttgctgag ataaatccaa tgtccaatat
     2281 aaaaatgaat attgacaaaa tcaatctaaa atattcttca aatgcaatac atctatttat
     2341 aaatacattc ttcaaaaatt ccatatcagt acaatattta gtccatattc ctataaatat
     2401 aattccacta tgataaatcg catatccaat aatagctact actaaagata atattaatga
     2461 ataaataatt gttcttataa tctttccctt tataagatta acactttctt ttcttgctct
     2521 tttaaaattt tctgtattta aagttatctc atgaatgcta aaaatccatc taataaccga
     2581 aatatatatt attaaaagaa aaatcatata gattatattt aaaattatat cagactttat
     2641 ataatctagt atgtactctg gtatttgtat ttttcctata aaccctgaaa ttaaaactgt
     2701 atttactaat ggaattatta atatcacaaa tataaataat agtatatttt taggatataa
     2761 caattttata gagttcttaa atgatatttt aaagagttca aataacccta ttttttcata
     2821 ttttatcgat ttattaaaac aaataattac agaggtaaat tcaaaaaatg cataaaaagc
     2881 tagtattata actgatagta ttatcagaac tattgacatg ggattggtca aaacttttat
     2941 aaaattatcc atatttaggt agactatatg taaacttttt attaaaatat caaatatata
     3001 tctaatagat ggccatataa aaaataaggc taatccttta tgaaataatt caaataaaat
     3061 taatgtacta aaattatatt ttattataaa aacaagttta cttaaaatat ttttacccgt
     3121 aaatataaaa tttcttaaaa ttctcttttg catttttatc tccttgtatc tatttgttct
     3181 aattttatca tatgttttaa atacatttag aacattaata tgtattattc caagttatga
     3241 tgtataaatg caaaaacaca tactcttttt tattgcatag ctaatcatca atatctatat
     3301 agcttaatac ttataagtag gatactccat atttacaaaa taaaaaagta tctcctttat
     3361 aactttcaaa aattacattg agatactttt tataatttaa atctatatac ttaatttaaa
     3421 aaacattgca taactattgt gctattatta caattcttca tataaatcag tacttaaata
     3481 ttttaaacca ctatcattaa gagttaaaac tatatttttt cctttttcac tgctctttaa
     3541 caacttaatt gctgcacata catttgcccc tgaagaaaat cctgcaaata caccttctaa
     3601 tttagctaat tttcttgcta catcagttgc ctcctcgtct gtaacttgaa tataaccatc
     3661 aataagtttc ttgtctacta atggcaaatc catagaatat cctccacctt gtattttatg
     3721 cccttggtca gttatttctt tacctgaata aattgctgct gtttcaggtt caacaacata
     3781 acatttgata gaagaattat gttttttaag tgtttttgaa catcctgcaa aagtactacc
     3841 acttccaaga aaatctacaa atacatcaat atttccatcc aattgttccc acatttcttc
     3901 tgctgtatac ttttcatgtg catgattaca agcttctaaa ttaaattggt ctgctctaaa
     3961 tgcatttctt tcttttgcta ttttttgagc ctcaatttct acaagtgcca aatcttcacc
     4021 agatacctgt ccatgaactg aattaggcat ttggtctaca agaactactt cagcacctaa
     4081 agctttcatc attcttgctc tttccataga atttcctttt gacatacacg ctataaattt
     4141 atatccttta catgcacaag ctattgctag tcctgttcca gtattaccac ttgttaattc
     4201 cacaactgct tgacctggaa ctaataaacc tgcattttct gcttcggtaa tcatttgtaa
     4261 tgctacacgg tcctttttac taaatcccgg atttaagtat tccatctttg cataaatatt
     4321 accttgaact ccaaattcat ctgctatttt ggataaatgt aacatatgag tattgcctat
     4381 agcctctgta acattattaa gtacacgcat tacaattccc ccttaatctc tcaactatca
     4441 tccgttgaaa aacaactctt tatgcagtat aatttatatt acggatgtac agtgtattta
     4501 acgttcatta tattatacat ttgtaaatta taaataatgt taaataaatt atacattcgt
     4561 aaattatatt tgtcaataat ttacatttta aggagctact atgagtatta atactgttat
     4621 tgctaaaaat ttaaatagat tacgtaatga acgtaactta agtctaggtc aacttgctga
     4681 attatctggc gtaagtaaag taatgctttc gcaaattgaa aagggagact caaacccaac
     4741 agtaaacaca atttggaaaa ttgcaagtgg actaaatgtt ccatatactg caatactgga
     4801 acaacctcaa aatgaaactt ttattgtatc aaaaactgat atagatgtac aagtttcaga
     4861 aaataaagat tatcgtcttt attgttatta tcccaataca ccaactagaa acttcgaatt
     4921 atttcaaatg gaattagaag aaggtcatag ttatacatct gttggtcatt ctgaaaaatc
     4981 acaggaatat attatgatta tagaaggtca attaaaatta gaagtaaatg actctattta
     5041 tcaacttaga gaaaatgact ctatttgttt ttcagctgaa tcaattcata cataccataa
     5101 tcaaggagaa aaaacattga aagcagtaat aataaattat tatccagttt agccatgcca
     5161 tttaataata gaataattat tttctataca aaaagataaa tcctttgatt aggatttatc
     5221 ttttttattt tatttttctt tataaattca ttatatatct ttacactttt atcaattatg
     5281 ttataataag c
//
LOCUS       DAEORZ010000206         5258 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEORZ010000206 DAEORZ010000000
VERSION     DAEORZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.211"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            95..592
                     /locus_tag="KRH39_003558"
     CDS             95..592
                     /locus_tag="KRH39_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG8513428.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(768..1016)
                     /locus_tag="KRH39_003559"
     CDS             complement(768..1016)
                     /locus_tag="KRH39_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513429.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(1082..2098)
                     /locus_tag="KRH39_003560"
     CDS             complement(1082..2098)
                     /locus_tag="KRH39_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG8513430.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(2156..2923)
                     /locus_tag="KRH39_003561"
     CDS             complement(2156..2923)
                     /locus_tag="KRH39_003561"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBG8513431.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIDLLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(2991..5180)
                     /locus_tag="KRH39_003562"
     CDS             complement(2991..5180)
                     /locus_tag="KRH39_003562"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBG8513432.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMGKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         1618 a         1006 c          527 g         2107 t
ORIGIN      
        1 aagttattta tgttataata ttaatcatta gataattaaa gcattgtacc taagttgcaa
       61 tatctaaaat tcaatttgga ggtataacta gcatatgtat aaaattggtg aaagtgtaat
      121 gtatccaaaa gagggagcct gctctgtaaa tgatattgta acaaagaaaa taaatcatga
      181 gatgcaaaaa tactatgaat taagtgtcat ttttaatagt aaccttaaaa tttcgattcc
      241 agttctaaat gcagatagaa ttggcatacg tccagttatg gatggaaatg atgttgataa
      301 ttttatccaa tctattaata aaacagatgg tgtatggatt tttgatagaa aagaaagact
      361 taaactgtac caagataaat ttcattcagg agatgttttt gaaattgtaa aattaatcaa
      421 aatgcttatg atacaagaca gttcaaaaca attgtgtagc acagataagg agtttttaaa
      481 taaagctcaa aaatttgctt tatcagaatt agctgctgct caatgtaaat cctatacaat
      541 ggtattagag gaaatgaaaa aacatatatt aaattcaaag aatacaaatt aaaccagttt
      601 tttaaactgg ttttattttt tatactaatc atccatgcta ctttagctta ttttaaactg
      661 cctaatctaa tattaataaa gttatctcaa ttagatagat gtattttatt caaataaaaa
      721 aaactaagag taaatcatat tactgattta ctcttagcta ataaaattta atcttcttca
      781 agttcttttt tctcttgtaa taatacactt aattcatttg cttctaaaac ttgtgcctct
      841 gcaatatcaa gtaactcttg ttgccattcc acacatttat ctttgtaatc aaaaggtagc
      901 gttctgtcaa ctatgtactt atcaatacag tcctccgcac ttttaattat ttcttttctt
      961 tcatcaataa atttttctaa ttctactaag gcttcttcat aagcttttct actcatataa
     1021 attccccctt tgtacaatta ttctacatta aaatgatttt tcctcttttt tgtataaaat
     1081 tttatttgta tataactttt ccatcaatat cttttatagt ttgacatcca gttaatatca
     1141 tagaactact tagctctgat tgaagtttat ttacataagt ttctacacca tcagttgcac
     1201 caccaaaaga agcagtcaca aaaggtcttc ctattaatac tgcatctgca cctaaaccta
     1261 tcatttttag tacgtctaaa ccagttctca ctccaccatc tgcaagtatt gtaactttac
     1321 ctttaacagc atcagcaatt ttgggtaaaa cttcacatgc tcctggtgta cagtcaagca
     1381 ctcttcctcc atgatttgat acaactattg catccacacc tgcttctact gccattaaag
     1441 catcttcaac agtcattata cctttcaata taaatggtag tttagtagac tttactaact
     1501 cctttatttg tttaacattt ttaggaagta ctggttttcc atgtaatgat aaagttacta
     1561 gaccacaggc atctatatca actccaactg caaatgcacc agcttcttca gctaatctta
     1621 tcttttttat aatattttca ttatcccaag gtttaataaa tacaattcca gctccattat
     1681 actttttgac tacatctaaa ttttccatta aaaaagcatc tactgccgta tcaccaacca
     1741 ttgcataaat accactatta gcacatcctg caactactgg ttctatgtaa tctctttcat
     1801 taatttttcc acccatattt aaagtagttc ctgttactgg agctgcaaat ataggtgcac
     1861 tcattttctt tccaaacatc tctatagatg tatcaggatt tgatacatca tgtattactc
     1921 tcatatttac ttttactttt tctaaactct tgctattttc tataaaagaa gaaccacttc
     1981 ctttacctcc catgccaggc acttcacctg cacaagcaac cccattacaa accttacaaa
     2041 ctttacaact tccatttaaa ttttctctag cactttttaa taattcatta tattccatct
     2101 ttaatccccc attaactata gtaatttata ttatatacat tatatcatat tatatttaca
     2161 gtatattttt caaaaaactt tttctgtgga taggagttat gccatatttt tcaatagctt
     2221 catagtgttc tttagttcca tatcctttgt gagatttaaa cccatattct gggtatactc
     2281 tatcatattg gtacattata ctatccctag taactttagc aagtatactt gctgctgcta
     2341 ttgatataga ttttgaatct cctttcacaa ttggattttg agatatgtca atacctggta
     2401 ttgttgctgc atcaactaat aaataatccg gtgctttttt taaacaattt atagcctttt
     2461 tcattgccat atatgtagca tttaatatat taaattcatc tatttcttca ttgtttacta
     2521 tacctattcc ataatctagt gcttcttctt ttattacttc aaaaagttca tctctttttg
     2581 cctcactcag tttcttagag tcatttacac cttctatttt ggtatctttt ttaaatacta
     2641 caacagaagc tacaacaggt ccagcaagtg gtcctcttcc cgcctcatca attcctccaa
     2701 tatataaata acctttatca tagccttcgt tctcaaatat atttatagtt tccaatcttt
     2761 cttcttcttt tctaatatta tccagctttt ttgccagttt tatagctaga ccttgtacag
     2821 attttctttc atcaactctt aacaggtcta tatattccat atatttttct acttctaaag
     2881 tctctattat ttctttaatc tccctcacac ttttatcctg cataccattc cctcatcttt
     2941 cgtttttaaa aatttaattt ttaaatatat taaaattttt taattagagt ttaattgcat
     3001 ccactatagc cacattttaa acatatacta catccacctg tatgtgcaat tccttcactt
     3061 ccacactcag ggcacacaaa tttttcatct tcttcaaaat ttttatttat tatttctttg
     3121 gctttttcat ctttattatc tttttctttt ttattatttt tataatattc ttttttacta
     3181 ttttcttttt tatcttcttt attattttct tccttttttt catttattaa aattccactc
     3241 cttttacatc catctctaaa tattgttact cctttaagac cagcctccca tgccttaata
     3301 tatatattga aaacatcttc aactgtagtt tcaaaaggta tatttattgt agatgatata
     3361 gaagcatcaa tatacttttg ccatactttt tgcatgttta ttctttcttc atatcctata
     3421 ttcatagcag taacaaaaaa atctggtaaa cattcttcat ctgtaatacc tttcttgtcc
     3481 atatactctt taacaattgg agtatatact ttataataca catcctcatc atgcaagctt
     3541 tcagtttttc ttatatatga aagattaaat ataggttcta ttccaccact gatacctatc
     3601 attgtagata tagaacctgt aggaggtatg gttaaaagtt gtgaatttct aagcccatac
     3661 tttttaacca tttccttagt gtcttcattt agattttcta taaaaaactt agatttagaa
     3721 atattttcat atttatactc actaaatgtt ccatattctt tagcaattaa tgctgattgc
     3781 ttcacagctt catttaacat agtcttacca attaaatcac atagttctaa agattcatca
     3841 gaaccatatc ttattcccat ttttataagc atatcagcta tacccattac acctaaacct
     3901 atctgtctat acttatgtac actatctctt tgttcttgta atggatgcaa gttcaatccc
     3961 tcatctaaga catcatttaa agctactaca gcttctctaa cgcaagattt tagtttatta
     4021 atatcaaaag tagcatgttc accaaatggt tgtattataa actctgacaa gtttattgag
     4081 ccaagtagac aacttcctcc acttggaagt ggttcttctg cacaaggatt tactcctgaa
     4141 aagcaaaagt tcttatcttc actcataaaa ttatatgttt ttattttatc ccaaaacaat
     4201 atacctggtt ctgcataatc ccagttattt ttcacaaatt tattaaataa atcataagca
     4261 tttactatct taactatttt ttctccagta gtttcaactt caaaagtaca attatataac
     4321 tttttttctc ttacagcttg cataaattca tctgttactc ttacagaaat atttgcttta
     4381 gtaaccttct ccaaatctgt ttttatatct ataaagtctt ctatctctgg atgatttaca
     4441 tccatagaaa tcataagagc tcctcttctc cctctttgac caattaagcc agttgtcata
     4501 ctgtaaagtt ccataaaaga gactgctcca gtcgtatgtt ttgcagcatt gttaactttc
     4561 gctccttttg gtcttagctt tgaaatatca attccaactc ctccaccata actaaaagtt
     4621 ctagcaagtt tttttgcagt atcaaatata tcttctatgc tatcttttgg tggttctatt
     4681 acataacaat tagaatatgt tacttttttt ccttctttct gtaatcctct atttgctaaa
     4741 attcttccag caaatagaaa tcttttttgt cttattagtt ttgatattct tgaactatta
     4801 tttgaaattc tctttatcca ttcatcaaaa ctctcaccat tatgtctata cttgttcttc
     4861 catattgatt tttgtaattc atcaaactcc caagctttag tccttatttc agagcgtctt
     4921 tctctatata atatgtagtg ttttgctata tcttttctat ttgattccat aagtagtact
     4981 tcaactatat cttgtatttc ttctacggat aagcattttc ccatagaatt atagtcatta
     5041 gctatttttc ttgatatatc atatgccaac tctctatcta ctccaccttc actatttata
     5101 ctagctttat agacagcatc ttcaatctta cttttactaa aaagttcttt tgtaccatct
     5161 ctttttatta tgtacttcat ttttaaacct ccaataaatt agtattaaat taatacacac
     5221 attaactata actctaaaca aactttttat atatttgt
//
LOCUS       DAEORZ010000207         5216 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEORZ010000207 DAEORZ010000000
VERSION     DAEORZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.213"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..682
                     /locus_tag="KRH39_003563"
     CDS             <1..682
                     /locus_tag="KRH39_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513433.1"
                     /translation="LLTAIFIFNNKNTNNKLKEVTKNNEEIVPKNLEGLSIEQLIEIR
                     DKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNYSNLTEKEKLIRQINIVN
                     YENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSYEGLDFLIDHLEELCKFV
                     DFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLSEEVKKEIKNWENNLNNL
                     NMNKNNSN"
     gene            complement(839..1045)
                     /locus_tag="KRH39_003564"
     CDS             complement(839..1045)
                     /locus_tag="KRH39_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513434.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
     gene            complement(1447..1842)
                     /locus_tag="KRH39_003565"
     CDS             complement(1447..1842)
                     /locus_tag="KRH39_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG8513435.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            1983..2375
                     /locus_tag="KRH39_003566"
     CDS             1983..2375
                     /locus_tag="KRH39_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8513436.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(2475..2891)
                     /locus_tag="KRH39_003567"
     CDS             complement(2475..2891)
                     /locus_tag="KRH39_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG8513437.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(2906..3817)
                     /locus_tag="KRH39_003568"
     CDS             complement(2906..3817)
                     /locus_tag="KRH39_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8513438.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(3999..5021)
                     /gene="splB"
                     /locus_tag="KRH39_003569"
     CDS             complement(3999..5021)
                     /gene="splB"
                     /locus_tag="KRH39_003569"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBG8513439.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
BASE COUNT         1852 a          805 c          581 g         1978 t
ORIGIN      
        1 tttattaact gctatattta ttttcaataa taaaaatact aacaataaat tgaaagaagt
       61 tactaagaat aatgaagaaa tagtacctaa aaacttagaa ggtttaagta tagaacaatt
      121 aatagagatt agagataaag ccattgataa aaatttagaa ggtcttaggg aatttggaga
      181 agattcagta aaaaaacgta aagattcaat tttaaacttt gaatttggat tagtagcttt
      241 tgttaacaag aattatagca acttaacaga aaaagagaaa ttaataagac aaattaatat
      301 tgttaactat gaaaactcta taaatgatat aatggatcct tcaggttcat tctttgaatt
      361 agcaaatcag aattaccaag tgaatgagtt tctgttaaaa aacggaaaag attataaaga
      421 atggtcatat gaaggtctag attttctaat tgatcatcta gaagaactgt gcaaatttgt
      481 tgattttgat gtaaaagatg taatagatat tattgatcct acaataaaaa cagatctttc
      541 agaggaacaa aagaaatctt tgaatgataa attaaagaaa atatcttcaa gtgatacttt
      601 aagtgaagaa gttaaaaagg agataaaaaa ctgggaaaac aatttaaata atttaaatat
      661 gaataaaaat aattctaatt aattttctca taagaaaaat gcttatatcg tgataatgtg
      721 aaatgtacat aattataaac aaaaaagggg ctgtgacact aaaaaatagt gctacaccct
      781 tttttatttg taataatcat caatacaaat tattttatta tatgtaatta tttatgtctt
      841 aaccaatttt atcgaaaata acagtcttct cttttaaaga gatttttcct acttgatatc
      901 catattcatt taattccttt ttatatttca aaaacgaatg gtctatctct atcccagcaa
      961 tatcttttat ttcttcaaaa gttaatttaa acgattgact attgtttttc ttaacatatt
     1021 cccataaaga actatattta ctcatcttat atcactcctt ttaaaatcat atcttatcta
     1081 ttaatttata ttctatcatt attaatgtat aaagtcatac tacttattat ttatatacaa
     1141 aaatatccaa ataataagta gcaatttttt atagtcataa tatttttttc agattaaata
     1201 aaaatcaatt atagttaaaa tatttttaaa agaaatctat cccattataa agtaaatttt
     1261 aattatctta tttctagtat gtaattattc aaaatagtat atcattattg aaatacacat
     1321 ctaataacta aaaattttta catattcatt atttaaaaat ctttttattg catttaaatt
     1381 aaatctatca atatttcagt aataacgttt tcttaatttt cactatggat gtcgattcaa
     1441 ttataattaa agctctacat caatcttttt accactcatt gaacaacaac taacttttgc
     1501 cttatcaata gagaatattt caaacttacc atctcccaca gagtacatct gacctaaact
     1561 tggcattatt tcaagtgcct tagcttgtgt ttcttttgat gtaacaaata ctgcttcacc
     1621 taaaatacgt agagtattaa ggttttggtc tatacaacaa agttcaacct ttggattaaa
     1681 aataagttgc ttaaacatat ccttttgatt agatgtgcaa aatgtaagtt taccattgta
     1741 ttccataaca aatcctaaag gacgcacatg tgcttgattg ttctcatcaa ctgttgctaa
     1801 ataataaact cctgccttgt tcaaaaattc taatgctttc ataattctta cacccctttt
     1861 aaatacttgt attaaggatt atcctttgat ataattttaa tataaaatac agaaaaaaca
     1921 agtatacagt tttttctgat aaagtataaa aaagatacta atataactaa ggagtaatta
     1981 aaatgagtat aaatactgaa aaaaatataa ctaaaaacgt tgaagaatta acctgtccaa
     2041 tccgttatgc attagacatt gtcggtggta agtggaaatt acctattata tgtatgttag
     2101 cagttgaaaa tccaattaga tatagtagca taaaaagaaa attggatggc attacaaata
     2161 ctatgctagc tcaatcatta aaagatttag agtctactgg tattgttcat cgtaaacaat
     2221 ataatgaaat tcctccaaaa gttgaatata cattaacaag taaaggtaaa agtattgtgc
     2281 ctattttaca acaatttgct aattggggtg ctactaatat gcaagaaaaa aacacatgtg
     2341 gacttagttg caaagaatgt agaaagataa agtaaattta aatttaccaa attattcata
     2401 ttatagacta tatattaatt tatctgattg aatataaaaa caggatgact tatatattca
     2461 tcctgttcta tatgttaagc tctaaattta attagttcaa gcatatcgtt taataccaat
     2521 aaactatcta cttgaatcat tagccattta cttccattaa aaaagtttac ctcattatat
     2581 atttttttga tataatcaca acaagtatta ataaataaat caacctctac tctattgctt
     2641 tcacttacaa taaccaaagc tttaaaataa ccttcttgtg gatataaagt acatagtgat
     2701 ttacctcttt ttttatattt tacattccaa cctctttgca ttgaacattt actgtattca
     2761 agcttaggtt ctacattata agtagaaacc aaagttttat ttaacttgtc ccataatgga
     2821 ttattaataa attcattaat ttgctcaaat gtaggtttag tatcttgaaa aaacgcatct
     2881 tgccaattca tattcatcat cctttctatt gttcaatagg aaccggaaca atccaatgtt
     2941 ccagatatgc tgcttcaggt gtagtggggt aatacatctc aattgcaaaa tcagtagttg
     3001 ttaaattatg tttcttcatc catctttcca taaatttatc tgctttaaaa acagctgaat
     3061 taactaactc attaaagttt tctgcctcaa atccacatac taaatattct ttagctggca
     3121 actcaaaagt actatatcct tctactgatg tacctgactc tacctctaca cctgccatat
     3181 acatacaatt tccttctttt gcatttccca tataaagagc agcaaattca tttccacctg
     3241 gaatttgatt tggtatatta tgtctttgac ttccaagttt catccataat tcctctacta
     3301 ttgcaattcc agttgtttca ccgcccacca actggcaaat aggtacttct atttctattc
     3361 caacaaatgt ccttggatta ctcaatgtct tacgattaac ttcaatcact atatcatctg
     3421 caatcagtgg aacaccttca tcaaccatag cataatttaa aattaattct ggcttaataa
     3481 agtgattgag ttttactgga tttgctctat atttttctgg agttaatcca tatgcttttt
     3541 taaaggaacg tgtaaatgtc tcatgactag caaatccaaa atttaaagct acatcaagta
     3601 tacgattctt atgttctgct aagtattcag aagctcttgc aagacgacga agctttatat
     3661 actccattac agtcttattt accaatcttt taaatagtct ttgatagtaa aatggtgata
     3721 aatttgcttt atttgctaat tcattaattg atatttcttc tgataaattt gcttctatta
     3781 aatccaatgt aatttgaata gactcccatg catgcatact ataactcctc ctgatatttt
     3841 tgttactctt atcctttata gtatcaaaac tagaaaaaat gttcttgata gatagtgccc
     3901 tttttaattt tcttatatga tagtgctaaa tctcattgct ttattataaa cataaaatat
     3961 ataaaaataa aatatagcaa atctttaggt aagatttgct atattatata ttttattaaa
     4021 ctatttttaa atactccatt tatttcctta agaaagaact ctttcatatc atctaattgt
     4081 tctttatcat acacgtactt accatatcca aattgaccgt atttaaactt tctatcttca
     4141 ttttccattg gcaatagtgt atctggataa atctctagta ttctattttt tgctttagtt
     4201 gtatatctat gagatataac ttcaaatata atttttttat caaaatcctt tggtaaagct
     4261 tcttttatac ttataatcag atttttatac tcttctttcc aaccttcata taaaaacaca
     4321 ggtgctatta tgaaaccaat ctgataacca gccctagcaa gttttacagc tgactctatt
     4381 cttttatttg cagaggctgt ataatgctca tgttcattta taattctagg agtattgata
     4441 ctaaatcgta tttctgtatg attattatgt tcagcatcca ataagctatc cacatcactg
     4501 tatttagtaa caaatctaaa tcttcctttt tcctctcttc caaaaaactc tattgttttc
     4561 ttcaaagaat tagtatatgg ctctactgga actgggtctg atgtagctgc cccttcaaat
     4621 atggttacct gtggctttct atcttcaata tatttttttg tctgttcaag tatttcatct
     4681 acatttacaa atacccttat aaatggttta tcacctaatt gtgtgttcaa ataacaatac
     4741 tcacatcttc ccatacatcc actgacaagt ggtagttgat aattagcaga aggtttacat
     4801 gtttgaaact tcagactttt tctaatccca actaccatag tctgctttcc acttctatat
     4861 tgttcatata gattttcacc tgggatatgt tctttaatcc tatttgatga tatattaatt
     4921 atctctacat ctttattatc tctaaattta ttgtatattt ttttacccac atcagtgtct
     4981 aaagttcctt tctcgaatat tattcttttg ggattaaaca tttatttcac ctctacttaa
     5041 agtttttaaa tcgaagaatt ttataaaata ttacttaatt aatcttccct tttttaaact
     5101 cctttattta ttagaaagta aaataaaagc ttaagacaag tttattgtat atataacttc
     5161 tatacacaat ttttttacta ttaattacgt ataatttttt attattaatt tataat
//
LOCUS       DAEORZ010000208         5175 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEORZ010000208 DAEORZ010000000
VERSION     DAEORZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.214"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            59..1522
                     /locus_tag="KRH39_003570"
     CDS             59..1522
                     /locus_tag="KRH39_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG8513440.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1578..2714
                     /locus_tag="KRH39_003571"
     CDS             1578..2714
                     /locus_tag="KRH39_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG8513441.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            2950..4065
                     /locus_tag="KRH39_003572"
     CDS             2950..4065
                     /locus_tag="KRH39_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBG8513442.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4217..5086
                     /locus_tag="KRH39_003573"
     CDS             4217..5086
                     /locus_tag="KRH39_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8513443.1"
                     /translation="MDYLKYFKKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         1979 a          538 c          985 g         1673 t
ORIGIN      
        1 tgaaatatag ctgtttaaat tattgtctat aaatataaaa tgaatggtgg tgagttaagt
       61 gaccgattta gttaacacca ttaatggaat tgtttggtca cccgtattag ttgcaatgtg
      121 tttgtgtata ggattatatt tttcattgaa attaaaattt tttcaaataa gagatgtaaa
      181 agaaatgttt actctcttgc ttgaaggaga tagttctcat gttggtatat catcatttca
      241 aggttttgct acatctatgg ctggaagaat agggactgga aatatagctg gtgtagctgt
      301 agcaatagca atgggaggtc caggagcatt agtatggatg tgtataatgg ctacattagg
      361 ttcagcaact gcatttgtag aatctactct agctcaaata tataaagatg agcatgatgg
      421 gcagtataga ggaggacctc catattattt tgaaaaaggt cttgggtgga aaaagtgttc
      481 agttttattt tcaatagttg caataataag ttatacattt tttatgcctg gtacacaatc
      541 aaacacttta tatctagcaa tgaatcaagc atttggattt tcaaaaatga caatagcagt
      601 agcatcttct attgcattag cgataataat ttttggagga attaaaagga ttggtttatt
      661 tgctgaaaaa gtagtaccta taatgggtgg agcatacctt attatgacat tagtaataat
      721 tataacaaat attagtttag ttccagaggc tattgtaaca atgtttaaat ctgcatttgg
      781 atttggttca gtttttggag gaatgctagg ttcagcaata tcttggggag taaaaagagg
      841 tatatactca aatgaagcag gagaaggttc tgggacttat ggtgcggcag cagcagaagt
      901 gagtcatcca gccaaacaag gattagttca agcattttct gtttatgtag acacattatt
      961 aatatgtatg gctacaggta ttatgattgt aataacagga atgtataatg ttacaggtgt
     1021 ttgcacagca ctgccaggtg tagaaccggg accacaatat gtacagagtg caataaatac
     1081 attacatcca caattaggtg atatttttat agcaatagtt ttattattat ttacatttac
     1141 aactttatta gccttctcat atatgatgga gactaatgta tcttacttag taactagaat
     1201 taaagcagaa aaacatgaaa agtacataat taatgtctgt agatttatgc ttatagcatg
     1261 tgcagcaatg tgttgtttta tagaaccact tacaagctgg gcattaggtg atatatgtat
     1321 aggtgtatta acatatataa atttaattgc catctttaca ctaaaaaaac cagctataaa
     1381 ggcatattat gattatataa gacagaagaa gatgggaata aaaaggagtg atagaacttt
     1441 taaccctata gagttagata taagaaatgc tgagttttgg gaaaatagat acttaaaaga
     1501 ggataagcat gagttaaatt aaagttataa taaaatataa aaattactaa ataaatttag
     1561 tgggaggtaa gattggtatg aaagaaagaa taatacaagt ggctgattca aagaaagaaa
     1621 aaattctagg gctatgtcaa tctttatatg atgaaccaga aattgctttg caagaatata
     1681 aatctgctaa aaaaatatca gaatttttaa gagaagaggg ttttgatgtt gaggaaaact
     1741 tggcaggtat ggctacagct tttaaagcaa ccaagaaaaa tggagatggt cctaaaatag
     1801 catttatagc agaatatgat gctcttccag gcaatggaca tgcatgtgga catcatttaa
     1861 ttgcatctat gggagttgga gctggaatag ctctttcaag tatactggat acttacaagg
     1921 gagaagtaag tataattgga acacctgctg aagaaactgg agatggaaag ccttacttga
     1981 tagaacatgg tgtatttgat ggctatgatg cagcaatgat gatacatcca aattcaaaaa
     2041 cttgtgtaac acctgaaatt atagcaatag gaggtctaga tttcatattt actggaaaag
     2101 catctcatgc aggagctaag ccatataacg gaataaatgc tttagatgca gtagtattac
     2161 tatataataa tataaatgca ctaagacaac aattagtaga tggaacgaga atacatggta
     2221 ttatactaga agcaggaact gctgcaaatg taatacctga tatgggaaaa gtaagattag
     2281 agataagggc aaaagaacaa aattactttg atgaagtagt agaaaaagtg aaaaattgtg
     2341 ctagaggtgc tgcaatagcc actggatgtg aacttgaatt ttatcacttt gaaccaactt
     2401 gccaaggatt aaatgaaaat aaagttttgg tagacatatt tacaaagatt atggaggaat
     2461 ttggaatata tgaagatgaa caagtaatgt taggttcaac agatatggga aatttaagtc
     2521 aaataatgcc atgtatacat cctttgatga agttttctga aaatggagaa gaactacata
     2581 caaaagaatt tttggaggct tctataaatt catatgcaaa agatagagta attgatggta
     2641 taaaaatatt agctttaaca ggatttaatt tatttgaaaa tccagagtta ttgaaaaaaa
     2701 tgaaggaaga gtagaactta gactattttt aattatactc tttaaaataa gaagagtgtc
     2761 tcaaaatgaa ctttttagtt tatgagacac tcttttttgt atgaaattaa atatattttt
     2821 attattttaa caatgaaaaa gaggcttatc tgtttttata tgaccttttt taacttgtag
     2881 cacaaaatat catgaatgat tacaattcga atgataaaat cgattataag atatatagga
     2941 ggggtacata tggaaacttt agaagtaaga aatattgaat tgagtggtac aaattatgag
     3001 ataggataca gactaggaga attggttgct aatatgccag agataataga aggtcagatt
     3061 aataaatcta atctggtaag caaaaaagaa gaaaaagaga tgatagagtt gtttgataaa
     3121 tattgtccag gtcttaatga agaattgcaa ggttttgctg atgcaattca agtgaattgt
     3181 aatcaaattt tatattatac tatgacttat cttaaaccag gttgtagtca agttgcatta
     3241 gcaccagaat taactgaaaa tgggcatgta ctatttgcac gtaatttcga tttctcacac
     3301 aatatggagg attttgtttt atgtaaaact aaggtaaatg gaaaatatgc acatataggt
     3361 actaccgtta tgcaatttgg aagaggtgaa ggtatgaatg aatgtggctt aggtgtaagt
     3421 cagtcatcat gtggtattcc tgtaggcaat tcagatggtt taagaaaacc agcaattgta
     3481 ggtttacagt tttgggctgt tattagatat ttgttagaaa attgcaaaga tgtagatgaa
     3541 gcattagaat atttaaaaga tatgccaatt gcatacaata ttaatttatt gcttgcagat
     3601 aagtctggaa acattgcact tgtggagact ttagatggga aaaaagaagt aaatataatc
     3661 aatagtttag aaagtaaaag agaatatttt ttacactcta ctaatcatac acatattgat
     3721 aaacttcata aattagagcc gcaatcaatg aaaaattcta ttcatagata taaattaatt
     3781 aaagaatata taaataagtc taaaaaaatt ggagagaaag agttaatgaa tttattgtct
     3841 tcaaaatatc caaatggctt atcttgtaat tactataatg atttttttgg aactttaaaa
     3901 agtatagtta tggatttaaa tataggaaag tttaatattt tatggggtgg actagagaat
     3961 aaatgggaga gctactattt aaaaaatgac attaaatcta taacacagag gataaacata
     4021 aatattgaaa aagcaccatc tgactttttt gatttcattg aataaaaaat gtatttaatt
     4081 attctaaata attaggatat ataaatcaac taaaagtttt tatttatcgt attgaattag
     4141 ttataatatt ttagtatagt aaaaaatagt gctattttac atatttaaag agtttaagta
     4201 gaagagaggt gccatattgg attacttaaa gtattttaaa aaagctgtaa tgtatattga
     4261 gaataattta catgaaaata taactgtaaa tgatgtggca aaagaaactg gatattctta
     4321 ttatcattta acacgcttat ttaagtctat gtttggtgag agtgtaggaa gttatataaa
     4381 aaaaagacga ttagtaagta gtacaaaaga acttttatat agtgataaaa aagtgataga
     4441 tatagctatt ggtagtggat ttgaatcttc ggaagcattt agcagagcat ttaaatctat
     4501 atacaaggtt agccctatag aatatagaaa aaatagaatt gatgtatttg ttggtaaaaa
     4561 gaaaaaatta gagttagatt ttatgagaca tcttgttgga aatataacga ttaaaccagt
     4621 tataaaagaa atagaaaaaa taaaggtaat aggtataaag gataaagtaa ttttagaaaa
     4681 taattcatta cctgacttgt gggagaaatt cagaaaagta catcatattg tcccaaatac
     4741 tttgccatca aagagagttt ttggaatctg tgaagcaatc tctgaaatac acctagttag
     4801 tgaaagtatg gaatttaatg agataatagg acttgaagtt aatagttatg atgtgattcc
     4861 aaattcattt gtttcaaaaa ctattaagga aggaaagtat gctatattta ctcatactgg
     4921 tagcttagat aatttagaca aaacttatga atatatatgg ggtacttggt ttttaagttc
     4981 aaaagaagaa cttgatataa gagatgattt tgaagtatat gatgaacgat ttttaggtcc
     5041 tagtaatgtg aactcacaaa ttgatattta tatacctata aaataatcta taagaaaact
     5101 aaaaaataat ttgattaaga aatagatgtt cgtaaaagag catctatttt ttatttttat
     5161 cagatttaga taatt
//
LOCUS       DAEORZ010000209         5062 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEORZ010000209 DAEORZ010000000
VERSION     DAEORZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.215"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            669..1700
                     /locus_tag="KRH39_003574"
     CDS             669..1700
                     /locus_tag="KRH39_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG8513444.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1833..3131
                     /locus_tag="KRH39_003575"
     CDS             1833..3131
                     /locus_tag="KRH39_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBG8513445.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPSNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3245..3490)
                     /locus_tag="KRH39_003576"
     CDS             complement(3245..3490)
                     /locus_tag="KRH39_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG8513446.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3638..4504
                     /locus_tag="KRH39_003577"
     CDS             3638..4504
                     /locus_tag="KRH39_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBG8513447.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4508..5044)
                     /locus_tag="KRH39_003578"
     CDS             complement(4508..5044)
                     /locus_tag="KRH39_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBG8513448.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
BASE COUNT         2040 a          513 c          800 g         1709 t
ORIGIN      
        1 gacatcttat agtttattga acctatagta atgaattaat aaatagttta attctatgaa
       61 aagatttcct tttatgcaat ttatttattg aaatatgata taatatataa aggtgtaagt
      121 attagttgtt aaaataatat ttatattagt ttttatacat acttggtata agtttttcat
      181 atattgatta aaattgatgt tgatttatta tttttagtat tttttcacct tttgattcat
      241 actttgtaga atggtgtatt ttctaaaact gattataaga caagttcatt caaattagca
      301 tttttatatc atataaataa ttgttccccc taaataagat ttagatgtat agtattaaaa
      361 tcaaaaagta taaaataaaa ttgatgctta atcatagata aatattaata aaatatacaa
      421 ggggattgtc taataaaaag ataatctcct tttttacaaa taattctaaa ttgtgatata
      481 aatctaaaaa ttttatctta acatttaacc atttaatgat aataattgat ataataaata
      541 taaattaaat atatggtgta cttattttat gtttttagca agaaatatag catttaaaag
      601 ataaattgtg caacttaaat caaagtttaa gatgaactat taacatagat atattaaaaa
      661 cttttgtaat ggagaatata tgtgatatga ataataaagt taaggttgca gtattagata
      721 caggaataga caaagaacat gattacttaa aagataattt agttggagga attgcttttg
      781 aatgtatgca tgattatatc tttatatcag ataaatttga tgatgaagat ggtcatggaa
      841 ctgcttgtgc atcaataata aagaaagagt atgaagatgt agagttattt gtaataaaaa
      901 ttttgagaaa agatggtatt acaaatataa aagttcttga agaagcatta agataccttt
      961 tagacactaa cataaggtta attaatttaa gtttatcagt cataggagta gaaagtgtta
     1021 agggcttatt tgaagtttgt tatgaattgt ttagaaaagg aaaaataata gtatgttcac
     1081 tcgctaatga ttttgactta agttatccag ctatgtttaa caatgttata ggtgtaagag
     1141 gatttacttt agatggagat gattcatttt ggtacaacaa aaaatatgat gttcaatgtg
     1201 ttatggatag caattcatat ataagttgtg atataaataa ttcctataga ttaccaccaa
     1261 aatgtaatag ttatttatca gcaaagctta ctggtaaaat agctaagata ttatcagaaa
     1321 aaccaggcat aacaatatct gatttaaatg ataagcttga atcattagca actagaaatc
     1381 attgggatag ttgtgatttt gatgagtgta gtagaattgt aaactttaaa ttggatttgt
     1441 atgacaaaga aaatgctctt cttatgaaag ttgctgacgt tgttagggat tgcttagata
     1501 ttgaagataa taatgaaaaa ctctttaagg gtagcctttt taataaggag ataggcttgg
     1561 tttatgataa ttgttttaat ctgttgaaaa agctagagca tagatttgac ataaagttta
     1621 attatatgga catctcaaaa tatgacttag tttcaatata tacactgact gaattagtgg
     1681 aaagatataa aaacttataa ataaagattt gtagctaaat ttaatgtaga aggtattaaa
     1741 ttatatgtat tctttgtcgg tagattaaaa gtaaaaaata tactataatt agatagcata
     1801 agagaaaatg ccaatacagg gggagttaga taatgaaata tgaagaagca ttagagtaca
     1861 tatcacaaac taataaattc ggtataagat taggattaga aaatatcgga aagttattag
     1921 aattattagg aaatccacaa gaaactttga atataataca tgtagctgga actaatggaa
     1981 aaggttctgt gtgttctttt gtttctaata tactaagaga gtgtggatat aaagttggtt
     2041 tatatacatc tccatattta gaaactttta ctgagcgtat aagagtaaat ggacaaaaca
     2101 taccacagga tgatgttgct agaataattg agttaataaa agaaaaaata gagattatgg
     2161 taaaagaagg ctatgcatat ccaacagaat ttgaagtggt tactgcaatg gcattttatt
     2221 actattcaga acaaaaggta gactttgtgg cattagaagt tggtttaggt ggcagatatg
     2281 atgctacaaa tataataaca aaatcacttg taagtgtgat agtatctata agtttggacc
     2341 atacaggtat acttggagat acaatagaaa aaatagcgta cgaaaaggct ggaataataa
     2401 aggaaaatgg agtagtacta gtatatgacc agactgatga agcaaaggat gttataaagt
     2461 cagtttgtaa agagaaaaaa gctaaatata tagaagttga ttttgatgat ataaacataa
     2521 aaaaatcaga tataaattct caaatatacg attgtactgt aatgaaagaa acttatagag
     2581 accttgaaat aaaattgata ggcgaacatc aaataaacaa ttctatcttg gcaattagtg
     2641 taataaaata tctaaaagac ataaataaat tagcaaatat aaatgaagaa tctataagaa
     2701 aaggacttat aaatactaaa tggccaggaa gaatagaaaa gataaaagaa aatccaattt
     2761 ttataataga tggtgcacat aatgaagatg gagcaaaatc tttggcaaaa gcacttgata
     2821 agaattttaa aggtagaaaa ttaaccttat taataggtat gttagaagat aaagatattg
     2881 atagtgtttt agagatattg ttgccacatt ttaataaggt aataacaaca acaccaagta
     2941 acccaagagc cataaattca gatatattaa gagaaaaagt attaaaatat gtagatgatg
     3001 taacatcaaa gcatgagata gaggatgcag taaattatac attagaaaca tcaagcgagg
     3061 atgatattat aataagtgca ggttcattat atatgattgg tacagtgaga actttagtaa
     3121 agaaattata attttataaa ataagtatac aaataaaaat ataaaaaaga gttgtctaaa
     3181 aaatggacaa ctctttttat ataaaataag tttttaataa ttttactgat gtttatataa
     3241 gtaattatat agattgacgt atagctttag ataattggtc tgggcaagaa gtaccttttc
     3301 caccacagtc aataccattt aatttatcag caacttcaag agcattttgg ccaattatta
     3361 agcttctaag tcctgataaa ttaccattac atcctcctat aaactcagca tcaacaatca
     3421 cattattttc atcaacttca aaaatcattt ctctacaaca tacaccactt ggattaaatg
     3481 ttactttcat taaaaagacc tccattaaat attatttctt tttttaacat taataataat
     3541 accaaatata tgcgtaataa tgcaactata aagcataata caagctaatg aaataatata
     3601 ttattgtagt atatgtattg tagagaggag gggttatatg aaaatctata tttctaacta
     3661 cttatcaaag agtattagca tagttgatta ttctacactt gaactagaaa aagaaatagt
     3721 attagaagat aatatatatc cacaccattt ttgcatagaa aaagaaaaaa atttaatata
     3781 catacctagt tcaagtaatg ggatactcta tgtcttagat ttaagtaatg ataaaataat
     3841 tgatactgtc tctataggag gaagtcttag tcaagttgta cttagcgata atgaattgtt
     3901 tgtggcaaat gaagattcta atagtatata tgtactagat aaaaacacat taaatccaat
     3961 aggaattatt ggagttgata atatgccaca tggatttgac tttgatagag aaagcaaaaa
     4021 gttatatgta ccgtgtataa actcgatagt atgtatagat acaattaata aaaatataca
     4081 aaagaaaata aatatggact ttagagcttg gcatatagaa cttgataaac aaaagaaaga
     4141 aatgtatata tcaacacttg atggaaagct tataataata gatgaagtaa gcatggatat
     4201 aaaaaaggta ttatatgaat ttttattacc agtagaaata agatttaatt atagtggcaa
     4261 aaaagtttat gttgctgatt tgggttataa taatgtaaga attttagatt atacaacgag
     4321 tagatacatt ggaaatattg aaataaatgg gattccccaa ggattagaaa tatctaaaga
     4381 tgaaaagtta ctatttgtat cagatacaca agaaaattca gtaaaagttt atgaaactgc
     4441 aaataacaaa ttaataaaag aaatcaaggt aggaaaagag cctacaacta taatttgttt
     4501 gtaggcttta atgtatgtct cttataagta aatcgataac ttcggcatac atataagatg
     4561 cattattttt ccagttattg caaataagtt ttgcttgttt ctctgatgaa acatttagat
     4621 ttaaatctat taagttagac tgattttcta tgactctaag gttgacaata aattcattat
     4681 cactttgttt tacaaaacta gactttactt gagtatctgc tagtagattt tctttgttag
     4741 aatctatata cttgtctatt ttttcagtaa cactagtagg tattcgatta aagaagtatt
     4801 ctagactttc tacgcctctt tgagtaagag cataatattc tctgtcagag tctttatatg
     4861 tagtcaaaaa cttagattcc ataagctgag ataaaagctg ttgtaatgaa aaataattca
     4921 tcatctcagt ctctaatact acttgagtta tttgagaatt agttaaatcc attttaattt
     4981 tttctaatat atataaaatt aaaagtttgt gataggctaa ttcttcagac gagttttcaa
     5041 acataaatat catcctccgt ca
//
LOCUS       DAEORZ010000210         5058 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEORZ010000210 DAEORZ010000000
VERSION     DAEORZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.216"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            182..2683
                     /locus_tag="KRH39_003579"
     CDS             182..2683
                     /locus_tag="KRH39_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBG8513449.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2843..3511
                     /locus_tag="KRH39_003580"
     CDS             2843..3511
                     /locus_tag="KRH39_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8513450.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3495..4772
                     /locus_tag="KRH39_003581"
     CDS             3495..4772
                     /locus_tag="KRH39_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG8513451.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
BASE COUNT         2114 a          531 c          896 g         1517 t
ORIGIN      
        1 ctattaaata caaataaata taactaatac atttatttat taaaaaaata ttgatttaat
       61 atacctatag ggggtataat aaaaatatag atttctaaat aagtaattta tcaatattac
      121 atatcaaaat aaaacgttac aaattagctg aaaatatatt gaaagaaagg aagattttat
      181 tatgagttca aataaagtgg tagaaagtta taaaattaca ggtatgacat gtgcagcttg
      241 tgcaaaggca gtagagagag taactaagaa gatggatgga gtatatgacc aaagtgtaaa
      301 tatagctact gaaaaactta aaatagaata tgataattct aaggttaatt ttgatgatat
      361 aaagcaggtt gtagaaaaag caggatatgg aattataaag gaagaaagta ataaaaaaat
      421 tgatatgaaa attgatggaa tgacatgtgc agcttgtgca aaagctgtag agagagtggt
      481 aaaaaaattg gatggagtag aaagtattag tgtgaatatt gcaacagata aagctaatat
      541 agactatgac ccatcaaaag taaaattatc tcaaataaaa gcagctattg aaaaagctgg
      601 atataaacca atagaagagg taagaaataa ggttgatgtg gatgaagata aattgagaaa
      661 agaaagagaa atgaagtctt tatttgttaa atttatagtt gcaatagtat ttgcagtacc
      721 tctgttttat atagcaatgg gacctatgat aataaagcct atagggccat ggcctttgcc
      781 agaaattata aatcctatga caaatacttt taattatgca ttaattcagt taattttagt
      841 aataccagta atgatagctg gttataaatt ttatataaat gggtttaaat cacttttttc
      901 tttaagccct aatatggatt ctttagttgc aataggaaca ttagccgcat ttttatatag
      961 tctatatacg acactacaaa tagccaatgg acaaattcaa ggtatgcacc atcatcagtt
     1021 gtattatgaa agtgcaggaa taataatagc acttatctta cttggtaaat atttagaatc
     1081 aaaatcaaaa ggaaaaacat cagaagcaat taaaaagctt atggggctac aacctaaaac
     1141 tgcaatagtg ttagttgatg gtaaagaagt tgaaactcct atagaagaag tagagatagg
     1201 agatatacta ttagtaaaac caggaactaa aataccagtg gatggagttg taatagaagg
     1261 atatacatca gttgatgaat caatgcttac aggtgaaagt ataccagtag aaaagaatgt
     1321 tggaagtaag gtgacaggag ctagtattaa taaaaatggt gttattaagt ttaaggctga
     1381 aaaaattggt ggagatactg ctcttgccca aataataaag ttagttgaag atgcacaagg
     1441 tacaaaagca cctattgcta aacttgccga tactgtatct ggatattttg taccaatagt
     1501 aattgcaata gctgtagttg catcattatt gtggttttta ataggaggaa aagatatagt
     1561 atttgtactt actatattta tatcagtact tgtaattgct tgtccttgtg cattaggatt
     1621 agcaacacca actgctataa tggttgggac tggaaagggc gcagaaaatg gaatcttgat
     1681 aaaaggtgga gaggcattag aatctgcaca taaagtaaat acagtgatat ttgataaaac
     1741 aggtactata acagaaggaa agccaaaagt aacagatata gtattaaata ataatgtaaa
     1801 agaagaatat cttatcaaga ttgcttcaag tgcagaaaaa ggttcagagc atcctcttgg
     1861 agaagcaata gttaagtatg gagaagagaa gaatataaaa tttgaaaaag ttgataattt
     1921 taaagccata ccaggtgctg gtatacaagt tactattaat gatgaaagta tattacttgg
     1981 aaatagaaag cttatgaatg acaataatat aaagcttggg gatttagaag aaaaatcaaa
     2041 tatattggca agccaaggta aaacacctat gtatatagct gtagatggaa atttatcagg
     2101 tataatagca gttgcagatg tagtcaaaga aagtagtaaa aaagcaatag aaatacttca
     2161 tgatatggga ataaaagttg caatggtaac tggagataat gcgaaaactg caaatgctat
     2221 agcaaatcaa gtaggtatag atatggtttt ggcagaggtt ttaccagaag ataagtcaaa
     2281 agaagttgaa aaactacaaa atcaaggaaa gtttgttgca atggtagggg atggaataaa
     2341 tgatgcccca gctcttgcaa aagcagatat agggatagca ataggaagtg gaacggatgt
     2401 agccattgaa tctgcggata tagtacttat gaagagtgat ttaattgatg ttccaacagc
     2461 aattaaactt agtcatgaaa ctataaaaaa tataaaacaa aacctatttt gggcatttgg
     2521 atataataca attggtatac ctgtagcagc aggaatatta tatgtatttg gaggaccttt
     2581 attaaaccca atgattgcag cagcagcaat gagcttaagt tcagtttcag tcgtatcaaa
     2641 tgcacttaga cttaaaaact tcaaggcata taagagagat tagattaata aattaaataa
     2701 aatgtatcca aaagagctat ctttaaatta aaaggattaa tttacaaatt aagatagctc
     2761 ttttttcatg taaaattcac aacaataaga taaaatataa gtaatatacg aaattttaaa
     2821 taaaatagaa aggattgata gaatgaattc gtcaatactt gtaattgaag atgattcaaa
     2881 tatacaagag ttaatatcag aatttttaag tgcagaaggt tatcaagttg atacagcaaa
     2941 tgatgggttg gaaggtatac aaaagtttaa gcaaggaagt tatgatttag taatattaga
     3001 tataatgatg ccaaacttag atggttatgg agtttgtaaa atgataagaa agtcatcaag
     3061 cgttccaatt atatttttaa ctgctttaaa tgatgaagga gaccaactta aagggtttga
     3121 cttagaatgt gatgattata taacaaaacc attttcattt aatcttctta taaaaagagt
     3181 agaagcaatt ttaaggcgaa gtaacaagac tataaatgac aaatttatag tttttgaaaa
     3241 attaaaatta gatttaaaca catatattgc agagatagat ggagaaccta tagaacttac
     3301 actaaaagag tttaatatat taaaagcttt gatagaaaag tatccacaag ttataactag
     3361 ggaaggtctt ttggatagta tatggggtta tgactattat ggagatacta ggattgttga
     3421 tgcacatata aaaaatataa gaaaaaaaat atcattacca tatattaaaa ctgtaaaagg
     3481 aataggatat acacttgaga aggatatttg ataagtggga aaagttaagt ataaagtata
     3541 agttattttc aataacgaca tcattattaa tagcattagc tttaataata tatctaattt
     3601 tatatttttt attgccatca tattatcatg agtataaaat tgagtctctt caagaaagtt
     3661 tgaaatcttt agtagatagt tcaattcatt tcgatacata tactttagaa gaaagacttt
     3721 attatatggc taaagaccaa aatttggcta ttttattaaa agataaccaa ggtaaaattg
     3781 tatatggtaa gaatgaagta gttattttaa gatatagtaa gtacatgatt aatagtcttg
     3841 aggatgagta tagaacttct atacctatat atactaaaga tgcaaaagat ggtccatata
     3901 ctttagaact cgttatgcca ttacaaccaa ttgacgaagc aaacgaagtt ataagaaaac
     3961 taatgccata catcatatca atagcaatat tgatagcgat tattggagca tatatatatt
     4021 caattgtaat aacaaagcct cttataaaca taatagagag tgagagagaa caggaatata
     4081 gaaggaagga ttttgttgct acaatatcac atgaactaaa aactccaata accataataa
     4141 gtggtcaaat tgaaggtatg atttatagtg taggtaagta taaggatagg gatacttatc
     4201 tgaaaaaatc gtatgagtgt acacaagaat taaaagattt agtaaatgaa atgatagagg
     4261 tttcaaaatc agaaatactt gaaaaagatt tgaaattggt atcaataaat ataagtgaat
     4321 tattaaatag acttgtaaag agacaagtat ttttaataga agaaaaacat atgaaaacta
     4381 ttttaaaaat agaagaaaat ttggaaatta aggcagacca agagagaata actaaggcaa
     4441 tcaataatat aataaataat gccataaaat attctcctga agaatcagag cttataatta
     4501 gactctatga taaaaataaa cgaataagta aaaagacttc taatcaaaga gtggttttag
     4561 aaattgaaaa tactggtgta actatcgaga aacgttattt agaagaaata tttaatccat
     4621 tttatagaat agaaaaatct cgttcgagaa aaactggagg aagtggactt ggactttata
     4681 tagtcagcca aatttttaaa agtcatgggt ttgattatag cataaaaaac aaagaaaact
     4741 ctgtagtatt cacagtagaa tttaaaaatt aattacttat tataaagaat atataacttt
     4801 caataatagt tcattttaaa atcacaaaaa attcataatg aaactgtatt atataaatat
     4861 aaagaagctt aaaaccataa aatacaccaa ctataatgat gagatttcct tacatcctcc
     4921 atataagtca tcattatagt aacccaacaa ttaagttact atgaatttaa aaaataacac
     4981 cgagcatata atctatgctt agtgttattt ttattttgaa ataaaaaata tttaatttat
     5041 tgaaatgtat agatttag
//
LOCUS       DAEORZ010000211         5037 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEORZ010000211 DAEORZ010000000
VERSION     DAEORZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.217"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(541..2676)
                     /gene="rnr"
                     /locus_tag="KRH39_003582"
     CDS             complement(541..2676)
                     /gene="rnr"
                     /locus_tag="KRH39_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBG8513452.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            3272..3886
                     /locus_tag="KRH39_003583"
     CDS             3272..3886
                     /locus_tag="KRH39_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG8513453.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTNRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            3904..4266
                     /locus_tag="KRH39_003584"
     CDS             3904..4266
                     /locus_tag="KRH39_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8513454.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            4372..4869
                     /locus_tag="KRH39_003585"
     CDS             4372..4869
                     /locus_tag="KRH39_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBG8513455.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT         1637 a          770 c          606 g         2024 t
ORIGIN      
        1 tatttcttaa ttacttatct ttcagctagc tttttctatt aaatgtttct caattactta
       61 tttttcaatt agccacttct attaagcatt tcttaattac ttatttttca attagtcact
      121 tctattaaac atttcttaat tatttatttt tcaattagcc acttctatta aacatttctt
      181 aattacttat ttttcaatta gccacttcta ttaagcattt ctcaattact tatttttcaa
      241 ttagccactt ctattaaaca tttcttaatt atttattttt cagttggctt tttctattaa
      301 atgtttctta attacttatc tttcagttag ctctttctat taaacatttc tcaattactt
      361 atttttcaat tagctacttt caataaatat ttcttaatta tttatctttc agttaactat
      421 ttccattaga tatttctcaa ttacctattt ttatcattta ttttatagag tattgtgcac
      481 tatatattta tacatatcat aaatactaca acttgttatc ccttatttta gctgttttat
      541 ctactcctct attttttcaa ctatctcaaa gtcaatttct ttaaaatcta cattaacact
      601 ttcaaccttt atcttaacaa ggtcacctat cttatatgac ttcttagttc tttctcctaa
      661 aacactataa gaactctcat cataaatata gtaatcatca ttcatatttg caagtcttat
      721 caatccttct atagtattat ctaactcaac aaatattcca aatgaagtta cgctagatac
      781 aacaccctca aattcttgac caactttatc cttcatataa agagccttat agaaatcatg
      841 tacatctctt tcagctaact ctgcctttct ttctttctcg gatgattgaa cagatgcata
      901 atccactata gtagtaagct gttcttgtct ttttccactt attttattat ttaaagattc
      961 ttttattatt ctatgaattt gaaggtcagg atatcttctt attggtgaag taaagtgaca
     1021 gtagaattta gctgctaatc caaaatgtcc actacattct ggtgagtact tagcttgttt
     1081 taaagaacga agcattatag tactgatagc tccttcttct tttttgccct ttattttctc
     1141 tattatacct tgtaatgctt ttggatgtac ttcctcttta tcacctttta ttatatatcc
     1201 aaatgtagat ataaacttac tcaattcttc cattttttcc aaagatggtg tctcatgtat
     1261 tctatacaca aacggtattc ccatccagta aaaatgttcg gcaattgtct cattacttat
     1321 aagcataaat tcttctatta tcttattaga tattcttctt tcatatggct ttatatctac
     1381 aacttcgcct tttccattta gaattatctt tgcctctggg aaattgaagt ctatagctcc
     1441 tcttttttcc cttcttgaca taagaatcct agctaaaatt tcagcatttt taaaatcttc
     1501 aacaacatgt tcaaaagtct ttttaagttt ttcatcatcc ttttctaata tatcagatac
     1561 ttctgtataa gtcattctag cttttgaatt tattacagat tcacatattt catgtttaac
     1621 cacatttcct ttatggtcta tttccataaa tatagaaaga gttaacttat cttcaaatgg
     1681 atttaagcta catactccat ttgaaagagt ttttggcaac attggaatta ctttatctac
     1741 taaataaact gatgtagctc tttttaaggc ttccttgtca agtttatttt tctctcttac
     1801 ataatgtgta acatcagcta tgtgaactcc tagtttgaag tttccattag gtaatacttc
     1861 tattgatatg gcatcatcta agtcttttgc gtcatctcca tctatagtaa atatgtttaa
     1921 atctcttaaa tctcttcttc tttttatctc ttcttgagga atctcaacag ctacagcttc
     1981 tgcttcttgc aatactttct ttggaaattc ttctggaaga ccatgttctc ttattataga
     2041 atctatttct acgcctcttt cacctttttg acctagtatc tctataattt tgccttctgg
     2101 ttttctatcc tcttgtggcc atactgtaat ttcacatact accttatcat tatctactgc
     2161 tccactaaaa tgttttttag gtatatatat atctttagta aatttcttgt tgtctggtgt
     2221 tacaaatcca aaagttttac ttgattggaa agtacctact attctagtta tttctctctt
     2281 tattactttt actattcttc cttctgctct cttttcttca gttgcaggta ctactatttc
     2341 tgccataact ctatcattat gcatagcacc atttatattg tcacttgaga taaataaatc
     2401 ctgtgtatat tcaacatcag attctacaaa tccaaaaccc tttttgtgtg atacaaattt
     2461 tccaacaaag tatcccattt gattgggaga tactattttt ccttttttag taaagcagat
     2521 ataaccatct tcttctaatt catttaaaaa attataaaac attggcattt ctgatgaatg
     2581 tatatcaaat atttctgcta attcttcttt ttttagagga ttatatgcac tttcatttat
     2641 taatcctagc aaactttctt taagtcctgg tatcattttt ccctcctgtt ttacttttca
     2701 ttatattatt atttatattg tcttttatat ttcttcagac aaaacatttt ttacttatca
     2761 cataattttc tatatttcct tattattatt acaagttaaa attacttatt tatatttttg
     2821 atttaatgta agctttgtca tactaacttc aaacaaatta ttatgtttta taaattattt
     2881 gtaaatatca ttataattac ttaagttatt ctttatcata attgcttaaa ttactcttta
     2941 tcacctaatt atacaccatc aaataggatt ggtaaaatat tcatctcata taagtattac
     3001 tatataataa tttattctat tagtatactc aaatcatgta aaatttatat aactcaatta
     3061 attttttaaa tacaacttta taatataaaa cttataataa aagtaaattt atatgttaaa
     3121 tgaggttgat tttttattat tttttattta tgctagtact aaagacttgc aaacgttttt
     3181 tgacaagtct ttattttgtc taaattaaac aaactttttt acaaataaat acataaaatt
     3241 taaactaatc tatttattgg aggagataaa attggaagaa gtaattgcaa aaattattac
     3301 tatagaaaat ggattcaaag aaattgaaaa cattgctaaa aaaattgtaa aaaataatga
     3361 ctcaaaaaag tgttattatc tatcaatcta cttatatagg tcagagtatt atcaagtaag
     3421 agaattggct gtgtttatac ttggttttat atcagtaact atttctgaag ctttgttatt
     3481 tttaaaagat aatgtgagta aagatgaaaa ctggagagta caagaaatac tagctaaatc
     3541 atttgattat tattgcagtg aagtaggata tgaaaaggca cttcctgtta taaaagaatg
     3601 gcttaattct gatagtccaa acataaaaag ggctgtgaca gaaggtcttc gaatttggac
     3661 taatagagac tattttaaat ctaatcctga cgttgctata tcactacttt ctagtttaaa
     3721 agaagatgat agcgaatact tgagaaagtc tgtaggtaat gctcttagag atataagtaa
     3781 aaaatatcca gatttagtaa aaaaagaatt agattcatgg gatatatcaa ataaaaaagt
     3841 tgagtttgta tataagcttg cttcaaaatg tatcttggca aagtaaaatg gaggtgaact
     3901 tatttgaaac ttcgtgatga gtatacttgc ccattagaac taacacatga tatgataaaa
     3961 ggcaaatgga aatctattat attatggaga ttacgcatag gaccaacttc cttatcaaaa
     4021 cttgaaaagg aaatcaatgg tattaatcaa aagatgttac ttgaacaact taaagaatta
     4081 attgaatttg gtcttgtaga taaaaagaca tatgaaggtt acccacttaa agttgaatac
     4141 tcacttacac ctattcgagg gaaacaaata ttggatgctt tggctataat gcaagatata
     4201 ggaattagct ttatgttgga taatggtatg gaagatattc ttaaggaaaa aggtgtttta
     4261 aagtaaatag atacccacaa aaaagtaagt aatttaccat tattttcaac tggtatataa
     4321 ttaaattata gatatattct attaatttat tataatagga ggtaaattat tatgaaagta
     4381 actagtacag gtatagtaaa tggtgttata gaagataaat acggtaaaag aggaactcag
     4441 ttcaatgaag gtggtatgcc tacatattca ctacctctaa agtttgaaga tgctccaaag
     4501 aatacagtat cctttgctgt atttttagaa gataaagact ctgttcctgt atgtgggttt
     4561 gcatggatac attggttagc tgtaaatata acaaaagatg agttaaatga aaatgaaagt
     4621 gtaacagcta ctgattttgt acaagggact actagttggc atggtaaact aggaggtctt
     4681 gacagattag aggccagtgt atatggtgga atggctcctc cagacagacc tcatgtctat
     4741 gaaatacatg tatttgcact tgatactatc cttgaccttg aaaaaggatt ctatatgaat
     4801 gaatttttca aagctattga agggcatgtt ttagatacat ttacactaaa aggaagttat
     4861 tctaattaat taggttgact atagctaatc attaattaaa tgctacattt aattaatgat
     4921 tagcttaatt gttattagaa tattttctgc actttatctt aatatatatt ttagttgact
     4981 ggtattttaa tttctattag attattatca atttatataa attgatttta gcttaaa
//
LOCUS       DAEORZ010000212         5036 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEORZ010000212 DAEORZ010000000
VERSION     DAEORZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.218"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            139..1476
                     /locus_tag="KRH39_003586"
     CDS             139..1476
                     /locus_tag="KRH39_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG8513456.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(1812..2381)
                     /locus_tag="KRH39_003587"
     CDS             complement(1812..2381)
                     /locus_tag="KRH39_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG8513457.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            2751..3357
                     /locus_tag="KRH39_003588"
                     /pseudo
     CDS             2751..3357
                     /locus_tag="KRH39_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
     gene            complement(4036..4938)
                     /locus_tag="KRH39_003589"
     CDS             complement(4036..4938)
                     /locus_tag="KRH39_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG8513458.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
BASE COUNT         1814 a          837 c          591 g         1794 t
ORIGIN      
        1 attttttgaa gcttaatttc tagaatttta tttttcaaaa tcaattcttt gaaatctaat
       61 ttccagaatt ttaattttta agatttaatt ttttaaaact tagtttccag aatcttaatt
      121 aatagaaagg tatattaaat gggcacttat tctttaatct ctacaagcat aaattttatt
      181 ctaatattaa tcaatatttt catagccttt tttctttata agttgacttc ctattttata
      241 gactttagga atcattggat tttcaaaatc atattgattt tgggctactc tattattagt
      301 caaatgataa tatggctttc cgaccccgta aatattttgt ttacactatt cggatatttt
      361 atactactta taatatgtag caaaagcaaa ttaattccta taatatcagt tgtcatgata
      421 ctttatccta ttataattgg gcttaatttt ctttttataa acaaccctat atatgaggaa
      481 cttatacact caaaagagta tattttaata ctttcaatag tccagataat ttctgctcta
      541 attaaattat tattttggtt tttaatacat attttcctaa agaatagatt ggtcaatata
      601 aaacaatact tatcaaataa aatatggatt tttattgata tggtgtgtat atcttcattt
      661 ttatcaatat taattgctat tattctgcca cctactaatt taatccctat gggtagtaat
      721 aagaatatta tcttaggcaa cttaccttat ggcacttatc taatcatctt gtcagcaatt
      781 ctggcaaata tcggtgtgat acttctttta cagccattga ttgaaaatgt aaagatgaag
      841 atagaaaaac aagcaaatca cctaaaagaa aaatactata acttactaga aaatcaacaa
      901 attcaaatac gcaaaatgcg acatgatatg aataatcact ttcaaatgat tgaatcgtat
      961 ttagaaattg aagatattgc tggagctaaa aagtatttta atcagttaaa aataggtata
     1021 ggtactctta gtggcaaaca attttgcaaa aaccaagctc ttaactcaat tttaaatctt
     1081 cgtcatacaa gtttagaaga aaatggtgtt gatgtgcact ttaatataga tatagacaat
     1141 cacttgggta tagaacctat agatttatgt actatttttt caaattcttt agataacgct
     1201 atagaagcat ctttgaaaat tcctgatttg tcaaatagaa aagtggtatt aaaggcacgt
     1261 tgtgagaagg agtattttag ctatttattg acaaacaaca aggtaaatcc tatcaataga
     1321 aaacaagaac tatttatttc agataaaaaa gatagtaatt ctcacggata tggtattgag
     1381 aatattagag aaattgttaa taagtacaat ggaaaattgg atatttctta tactgagagt
     1441 gaattttctt tatttcttta tattagatta ttttagtcta aaattagatt atcttatata
     1501 atacaaatta gattgtttga tactatgtaa aattataata acttataatt aaatatttac
     1561 acatatattc attaacttaa aatttaatca aatgtgatat tttatttacc ttagtaaaaa
     1621 tgtgtgtgtc tcaaaataaa ctttttagtt gatgaggcac tcttttttac atgaaatcaa
     1681 atatattttt atcctttcaa caatgaaaaa gggatttatc tctccatttt gaaacaagcc
     1741 ctttttctat taattacttt cttaaaaaat ataatacaat atataagata aatatctaat
     1801 tgtacctatt tctaattata tctattagaa tacaacacct ttgccttttc agtaaactcc
     1861 ctaacaaaat ctagtttact atctgtataa gcatcacgat tatgctcata cttcttccaa
     1921 aggtccaact taagtttttc atactccttt gcaacatctg ggtggtcaat taagtaatct
     1981 ctaaaaaaca actcatcatt atcacctaca taccttatat gcaaatgaaa aactcgttct
     2041 tcaaacccat ctactgtata ccctttatta agtgaaagcc tactactgct ctcagacata
     2101 caaatatatc cattcgactc aatcaaatct ttaatatctt tcatattata atcaggaatt
     2161 atctcaatca gtatgtcaac aatgggcttg gcataaatcg tactcacaga agtacttcca
     2221 atatgactta ttctaacaat tttttgcatt ggcaatattc cagttagtct atattgttct
     2281 tctttaaacc aatttttcca acattctttg tgagtagtca aaaatatagg aaacaagttc
     2341 cataactcct ctaaagtcat atcttccaac ttctttttca ttagttacct ccttttaaat
     2401 ttgtacttta tattttttct ttttcaaatt tacttagtta atatttaatt ttatttttta
     2461 tcaaatcatt tttctaatta tgtaaatttt tctaaattta gtttttaatg tattataaca
     2521 gatttttacc cttcttacac ttttgtatct atttaaatat tagaacattt tttcttactt
     2581 ctaaatacag taagattgct taaataccaa ataggagcaa aaaaactctg taatctaaaa
     2641 aaaactacaa catctttatg agaattaaaa aatgatagac tatgttatca acattaagaa
     2701 acagatagaa aagaaacaga gtatgtgcta aaatgggaac atagaacaga atgacaaatt
     2761 ggaatttatg gaggacatta aatatggagt atataatcaa tccttgtagt ggtataaaaa
     2821 ttaaaataac tagagggcag aaaattgaaa ttgtggatat tgaagaagga caggtcgttg
     2881 attttttttg cagaaatgaa agatgattca actgaattta tatctccagg tgttacaatt
     2941 gattgtaatg agtcattaag attacaagtt gggaatttaa tatattctaa taagtataga
     3001 cctatgttta aaattttata tgatgatgta agggaacatg acttgctaca tccatgttgt
     3061 agacctgaga tgtacgattt cttttatcac aatggagaaa atcatccaaa ctgttttgat
     3121 aatattaatc gcacacttgg aacttcccat caaattattc accccattaa tttgtttatg
     3181 tacacaaaag tcaatacaga tgggagtatt gaagtatgtg aacctttatc aaaagccaat
     3241 gacaaaatta ttcttgaagc attggaaaac atgactttag ggattgctgc atgcagtgtt
     3301 tcagaaagtt cttgtaatag cagaaaatgt acttctatta agattttggt tgattgacaa
     3361 atttcattta tcgaaaaaat atcaaaatta aaaaatgacg tcaaaggcgc aaaagggtaa
     3421 ccacttgcgc tctcaattca ttatggtttg ttacgtattt atgggtaaaa atcccttgat
     3481 tctatgatgt tcttcagaca caattacaaa tgagcgagga ttttatactc atactctaaa
     3541 aaatagcatt ttattgcgta acagaacgaa aaaacagtgt gtaccttttt cttgcaatac
     3601 acactgtatt catagccact ctagatgtta actgttaatt atctcataat acccttactg
     3661 gtattcatct aatcttaacc ccttttaatt attttcattt ataaataaat tctattcttc
     3721 ccaaaatatc gcaattaaaa tatattaatt aaaatatccc aataagaata tctgaaaaat
     3781 cttccaccta ttttttcaaa ccctttattc ccattcctca tacaatagtg caacttttag
     3841 cacagtataa atataccatt agtccatata atctcattaa caaatatata caattatttt
     3901 cttcaaactc ttttacatct aaaatatcac gttttcattt tcttatatta attccttaac
     3961 tctctttcaa attaccaatt ttttaatcaa atcttcatta aatattctat cattattaaa
     4021 cattcatcat tatccctaaa aaatatgtgc cgtatgttct ttacacaaat tttccatttt
     4081 atcatacaca gcaccactat ctctctcatc tgtcatagtc acaattacat cacttggtct
     4141 aagaattgtc ttacctctag gaataatttc ttctccacct ctttgaattg ctaccaaaag
     4201 acaattttca ggccactgta catcctgaat tgccttgttc gcaacaggag agccaaacat
     4261 aacagagaac tggtctagta tcttttcgcc cctctcttta tgtacaggtt ctccattttt
     4321 ttcaagtaac ctctcaagaa gactttcata aataggttta gacttcataa gtgtagccac
     4381 aatatatgcc acaattgaaa ttactgatag tgaaagcatt tgactaacag aacctgtcat
     4441 ttcaaaaatt aatatgatac cagtcaaagg ggcacgcaca atagctgtaa aatatcctgc
     4501 catagccaaa agcacaaagt tatttacata atcgggattc attccaaaaa ctttaacacc
     4561 taccatacca aatattccac ctataaatgc tcctaaaaca agcaatggaa aaaatattcc
     4621 ccctggtgca cctgacccaa aactaacagc cgaaaatata aaacgtataa caaaaatcaa
     4681 aacaaccatt ccaaaaatat attcatgact tgtaagttct tcaactaaat tgtgtccact
     4741 ccccagaaca tcaggcataa caagcccaag aataccagcc attataaatg gaatcaacag
     4801 ttttccaaac gtaccaattt taggaatctt cttgtataaa gactgcacat atagagtaaa
     4861 ccagttgtaa aatgccccca taactccaag aataacacct aaaatcacta ttaatccata
     4921 ataatcttgt ggcaacacat ttccaatctg aacctcccat gactaaagtc acagggttcc
     4981 tgcttcatag aattttgcat actaaaatat gtcttactaa ttctccacag gctatc
//
LOCUS       DAEORZ010000213         5032 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEORZ010000213 DAEORZ010000000
VERSION     DAEORZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.219"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            606..2387
                     /locus_tag="KRH39_003590"
     CDS             606..2387
                     /locus_tag="KRH39_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBG8513459.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVISIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDTEQDYQVNDFVGDLNKVYLAMSRA
                     IDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(2469..2999)
                     /gene="lepB"
                     /locus_tag="KRH39_003591"
     CDS             complement(2469..2999)
                     /gene="lepB"
                     /locus_tag="KRH39_003591"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG8513460.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEILG
                     KVMVRLLPLDNIGKVD"
     gene            complement(3039..3863)
                     /locus_tag="KRH39_003592"
     CDS             complement(3039..3863)
                     /locus_tag="KRH39_003592"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG8513461.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            4120..5028
                     /locus_tag="KRH39_003593"
     CDS             4120..5028
                     /locus_tag="KRH39_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG8513462.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
BASE COUNT         2038 a          601 c          680 g         1713 t
ORIGIN      
        1 ccaatagttt tatatattat tgattttatt aaaaaaagag atatacaaac taataagttg
       61 taatctctca aatataaaaa aacaataaat tagctcattg gattttttag tccataatat
      121 aattatagat tattcaacaa acaatctata gaaattgtgg tattaatgtt taaataacca
      181 actttttcta aaatagcctt tataggaata tatgagttca taggtaaaga tagcgaaata
      241 agaaaccagt ttggtatgta aaatctagat ttttaattaa attatcaaaa ttatatatta
      301 tagtatcaaa aatagaaaat taaaattaaa ttttttataa taaatcttat ttttagtcaa
      361 caagatttac tttttttaaa actttactta ttattttaag cattaattat gaaatttatc
      421 attttattat ttacagaaag tattttataa caataaatat ctatatttat tgcaaaatta
      481 ttttagcagt tttgtattta tttgtattta ttttgaattt atttactaaa tttgcctata
      541 aaaagtatat ataaaaatat agttcaaaat ggtataatta agtataacag aaggaggtaa
      601 tgtaagtgag ccaatacata aatataaatt ctcagttaga cgccctaaaa ttgaaggagt
      661 tcgagaaaat attttacgaa aatatcaatg aggacgttaa agtagtgcca aaagttactc
      721 cttttaaagg aataaatacc gatttgttgt atgttaaaga tggaagaatt ttatttataa
      781 aatttatgga tacaacagaa gatatatttt ttattttaga agaagagtta cttgaagtta
      841 tgaatgagga atatgaactt ttgaaactaa aaatgggaca aaagaataga aatataagtt
      901 ataactacgt ttacattatg ccatacgttg aagttgagga aacatatgaa tttgaagaat
      961 ttgttaataa taatataata gataaaaata agcttcaaga tataatgaat aagggttcgc
     1021 tggaggaata tcttaatgat gagaatgatg aaataaactt aaatttattt ttactagaca
     1081 tatgttcaga atactatata ataaatgata aacttcatct aaatgaaaaa tttaaaaaaa
     1141 tttcatttta caatgatgac tataagtata cagcaactat gatggaagac gttcaaatta
     1201 aagatgttat ttctattaaa tatgggaata cacttattga aggtggttct ggtataggca
     1261 aaacagcaat tatgctttct agggctataa aattagcaaa agtatatcca catcataaat
     1321 tagtcatatt tacacataca aaacaattgt gcaatgagct tcgagagagg atagagttat
     1381 tatacaaaga taataacaat ttagaagttc acacatttag ttcttttata tttaaactag
     1441 ctaaaaaatt caatttaatt gtagattata acatgttaaa aaatgattac gaaaagacct
     1501 ttaataatct tgtaaaacaa gcacagaaca ttataaaaaa taaaaatatg ttcaaggcta
     1561 tttttattga tgaagcagaa agctttttag agtatgaaat agattttata agggagtttt
     1621 tatataaaac aaaattcata tttaatgtgt gttcttgtaa ctccttgaat atctctaata
     1681 gattgaatat atttaaaaaa ttatataatg gtatagaatt tgatgataag attatcttaa
     1741 gtaaaaatta tagacaagct aaagagattg tagattttac aaataaattt tcaaataatt
     1801 ctaactcata tatcaacgaa ttaagaccaa atacagaatt tagtactttc ttttatacta
     1861 aagcacttag aggtggaaat aagtctgttg atataataaa agtaagtgac cttgatgacc
     1921 aaataagttc tgtaatctgg gaaattgagt atttaataag taaaaaaggt cttgattatt
     1981 ctgaagttgc tatcgtttat ccttacaata agaagaaatt aaaaagtgga aaaaccattt
     2041 actttcaata tatgttaaaa aaagcattgg aagaggcaaa aattccttat atatgtgcag
     2101 aagataattt gacaaatata agtaagaagg taggaactac gatagcaaat atatatgcaa
     2161 taaagaattt agaatataaa gcagtaatag tatgtgaatt agagatgtta tacaatcaaa
     2221 ctataaatga taccgagcaa gactatcaag taaatgactt tgtaggagat ttaaataagg
     2281 tttatttagc tatgagtaga gcaatagact atttaagtat agtaacgact tttaatgaag
     2341 aagcaagtga tataataaga cttattacag aatcaaaaga tatttagtaa aaaaatattt
     2401 ttaatttaag aaaataaaaa gagactgaga tttgtaaaat aaactttacc aattcagtct
     2461 cttatttttt agtctacttt cccaatatta tcaagaggta atagtctcac cataacctta
     2521 ccaagaattt catcctcatc aaccatacct acatcaggga atctactatc attactattt
     2581 tctctattat ctcccattgc aaatagctta ccttttggaa caacagtatc tatatctcca
     2641 gaagtatagt tattgtgtat atatggttca tttaataatt ttccatttac atacacttta
     2701 gaatttgata tttttattct gtcaccttca gtagctataa ctctttttat taagtctttt
     2761 ttctttcctg tttctccatc aactaaattg gttttaaaaa ctacaatatc gcctttttca
     2821 ggtttaccaa ccttgtatga tatcctattt aaaatcagat aatctttatc atctaaagta
     2881 ggatacattg attctccact tacaatagaa ggtcttataa attgtagaat tacaaatgca
     2941 agtacaatag ccatagctat tgacttaatc caatcaaata tttctttttt aacactcata
     3001 acttttctcc ttatcatttg ttattaaatt agaatacatt ataatagaaa gaaataagca
     3061 agaaccacta tacccaagac tattctataa taaccaaata ctttgaagtc atgttttttg
     3121 atatagtcca taaaaaattt aataactaca attgaaacaa caaatgctac tacagaacca
     3181 acacctaaaa taagccattc aaatccagta aatccaaatc cagcttttag aagttttaat
     3241 gcactagctc caagcattgt agggatggct aagaagaatg aaaactctgc tgctacatat
     3301 ctagaagtac caagtaatac tgcacctatt atagtagaac cagacctaga tgtacctggt
     3361 ataagagcta gacactggaa taatcctata caaagagcta atttatatgt aacttgagaa
     3421 aaatctttat acttaggttt tttatttctc ttttcaagcc ctatcattat tataccatag
     3481 acaataagag caatagcaac tactgttgaa ttaaagaata atctatctat atcatcttca
     3541 aacagtattc caataactcc agatggtata actgcaatta taactttaag ccataaacga
     3601 actgtttctt ttttctgttt aatatttttt gcactgtcaa aaggatttaa ctttctaaaa
     3661 aatataacaa gtacagctaa tattgaacca aattgaatta cgactaaaaa tgtacttata
     3721 aatgtatttg aaaaattcaa ttttataaac tcatcaacta atatcatatg ccctgtacta
     3781 cttactggta gccattctgt tattccctga actatgccta aaatgactgc cttaaaaatt
     3841 tcaagcaaac taaatgtttc caaatcaaaa tacctccaat acaaatttat agtagcatat
     3901 tttattcata gattaataaa aaaatgccac cttttactaa cttataatac catagtactg
     3961 tgtagtgcac aatggaagat tgatattttc ataaaacctt cataatataa caaaataaac
     4021 ttaaaagtta tctattgaat aaggggtata tttgaggggg aaatatatgg tatagtaaaa
     4081 gtatagctat tttattttat gaaaaggaaa ggagttatta tgtatagtac aaaaatacta
     4141 gatgattatg agtatgaaac ttttaatgtt ttttctacaa aattaaatcc aaacacgaaa
     4201 cacgattatt tatcaaaggt tatactgttt aaagaatttt taaaagggaa agaattgatt
     4261 tatgctacaa aagaagattg taaaaatttt gtagactata tacagactaa atatgcgaaa
     4321 tcaacttgtg aaaagatata tagttattta catagttttt ataacttctt aaaaaaagaa
     4381 ggatacatag atataaatcc atttagatat gtagaaaaac ccacagtaac aagaataaag
     4441 acaaaagatg atgttctaag tatacaagaa ataaataaac ttataggtat cctaccaaaa
     4501 ttaaatataa gagatagagt aataatagtc tgtctagtta ctactgggtg tttattaaat
     4561 gaattagtta gcttaaagtg gaaagacctt atggtagatg aaaacgataa ctcatatgta
     4621 aggttaggta agggaagaaa agaaagagta gttaagttac atccatactt ttttaaactt
     4681 ttagaagatt acaggaatta ttctgggctt ccagaggtaa taatacctag tgatgatttt
     4741 atttttacaa ctcaaaagag taattctata acagatagaa atgtcagact tatagtcaaa
     4801 aaagcattag atttagctgg gctttctcaa tattcagcaa gagactttag acattcattt
     4861 gcagcaataa gtttaaggct tggggcagat gaaagtgatg taaaaaatca gcttggatgg
     4921 agtgataaat actatgcaat tagatataag tatgtattaa attttgttga tagtgaaata
     4981 gtggattact taattgaaaa agataattta gatataaata aaaaatagta at
//
LOCUS       DAEORZ010000214         4953 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEORZ010000214 DAEORZ010000000
VERSION     DAEORZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.220"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            32..337
                     /locus_tag="KRH39_003594"
     CDS             32..337
                     /locus_tag="KRH39_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513463.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            745..1434
                     /locus_tag="KRH39_003595"
     CDS             745..1434
                     /locus_tag="KRH39_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8513464.1"
                     /translation="MDILLVEDDRNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            1434..2351
                     /locus_tag="KRH39_003596"
     CDS             1434..2351
                     /locus_tag="KRH39_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8513465.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            2424..3350
                     /locus_tag="KRH39_003597"
     CDS             2424..3350
                     /locus_tag="KRH39_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513466.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVNPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            3347..4027
                     /locus_tag="KRH39_003598"
     CDS             3347..4027
                     /locus_tag="KRH39_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513467.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIIFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            4125..4460
                     /locus_tag="KRH39_003599"
                     /pseudo
     CDS             4125..4460
                     /locus_tag="KRH39_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
BASE COUNT         1874 a          517 c          795 g         1767 t
ORIGIN      
        1 agtagttaat tattaataag gaggggtcaa aatgcctagc atgattatgg aagttgtagg
       61 agtactattt atagctattg gattaatctt gacattcatt gaaataaaag gaaatttccc
      121 ttctttttat ggttttggtc aaaattctat agggaaaaat gctgtaggta ttatagcagc
      181 aggaattgct ttaattttct ttagaaatgg catagatagc atagattcta tagctactta
      241 tggtgtattt ttagcaatag aacttgttgt aatagttgca tatttcttaa taagaaaaaa
      301 atttactaaa gaagataaac atttaagaac taattaacta aattatctac tataaaattg
      361 gaaaattaaa aacaaataaa agtttgtttt taattattta aaatccaaat ttgattattt
      421 ttaaagaaac tatgttctgt ataaaactag aatgtggttt cttttttatg tataaaatat
      481 tttttagcaa aagttaaaag gtgttttata atataaaata gttaattgta gatgttaata
      541 aaagaaaatt atgtatattt cagataaaga taagagagta tactatattt taattctttt
      601 ttcaataaag cttattgaaa agaactttaa cttagaagag tatcaccaaa ttgcagatac
      661 aatatcataa gcagttattt attgacaacc ctttttaata aatatatcat ttgaagtata
      721 ctgacgaaaa ggagttgaat aataatggac atcttattgg ttgaagatga taggaatatt
      781 cgagaaatgg tatacgattt tctgattagt gagaattact ctgttaaaac tgccattgat
      841 ggaaatacag ctattgaaat atttaaaaat tattcatttg atattatttt actggacttg
      901 atgttaccaa agtctagtgg ttttgaagta ttaaaatata tacgaagtgt gagtgttgtt
      961 cctgttatta ttactactgc gaaagatagt gacacagata aaatgctagg gcttaatctt
     1021 ggtgcagatg attatattac aaagcctttt tcaatcgtag aattgcttgc aagaattaaa
     1081 gctaatatta gacgtgcaac agtgtacagc agttcaagaa actctcaaga tatggttatt
     1141 cagtataaag atttgattat aaacatatca aaatatgtag tttcaaagaa tggaattgac
     1201 ttaaacttaa cacatacaga atttgaaatt gttaaattac tagcgactaa tcaaggacgt
     1261 gctttttcaa aggaacaatt atacaacact atttggaaag aaccttatta tggaaatgaa
     1321 aatgtgctaa atactcatat taacaggtta agaaataaat tgtctgatgg tatagaaaaa
     1381 aataattata ttaaaactct ttggggaatt ggctacaaaa tggaggaaga ataatggagt
     1441 tatttttagt tgtaatttct attgtactat ttgctctcta cttaaaagaa cgaaataaat
     1501 caaagaaaat ggaaagtgat tataagtata tcaattcaag acttcgtgac ataacaaatg
     1561 atgatgaaat taattatatt ttagtaccat ctgatatttc gattgtaaaa gaaacagcag
     1621 tagatataaa taaacttctt gaaaagtttt acagcaggca aattaattat aatagaagtg
     1681 aaaaagcgat attgcaaata tttacaaaca tatcacacga tttacgaact cccatcacag
     1741 tattaaaggg ttatattgaa atgctatatc ttcaaagtca aaaagaagaa ttatcttctt
     1801 caatgagagc aactgtggaa aaaatgcaaa caaattcaaa tgagcttgtt aattcaataa
     1861 ataacctgtt taatatggca aaaatccaat ctggagatat gatattaaat cttcaaaaat
     1921 ccaatctaac acaattatgt catgagatta ttttagagtt ttatgatttg ctagataaag
     1981 aaaactttaa tgttgaagtt aatattgaag atagacctat atatgcaaat gttgacacag
     2041 aagcattaca acgtatactc aaaaatttaa ttgataatgc tataaaatat ggttcagatg
     2101 gtagattttt agggatttca ctatatgaaa aggaaaaaca tgtttttata gatattgagg
     2161 ataaaggttc aggtatttct gaaactgaaa aagagcatat tttcacaaga gcttatactg
     2221 ctgacagaaa aaaaggcaat ggtttaggtt tggctatttc tcaagggctt gcaaactcta
     2281 tgggaggttc aatttatgta aatagtagac ctaatgtaaa aacagttttt acggtaaaat
     2341 tgaaaagtta gaaaaaagtt agatttaaaa gagagaaaag ataattttat tttgtatact
     2401 gtttttagaa aggagggagt atgatgaact atatcataga gacccatcaa ttaaagaaaa
     2461 tatataaaga taaagctgtt gttaatgctg ttaatattca tgtgaaaaag ggagaaattt
     2521 atggatttgt tggaccaaac ggggcaggta aaagtacagt aatgaaaatg ttgctaaatc
     2581 ttgttaatcc aaattctgga gaaatagtta tgttcggtaa gaaagtggca gaaactgatt
     2641 ttgaaatatt aaaaaagatt ggtactatta ttgaaaatcc gtatttatat gagaatttga
     2701 ctgctaaaca gaatcttgat ttacactgtg aatatatggg ttattataac aaagaacata
     2761 tatcagaggt attggaatgt gtcggattgt caaagcaatc aaacaagaaa gtttcaaaat
     2821 attctttagg gatgaaacaa cgtcttgcaa tagctagagc aattcttact aaaccagaat
     2881 tattgattct tgatgaacct ataaatgcac tcgacccaga ggggataaga gaaatgcgag
     2941 aattattccg taagttaaat acagattatg gaataactat atttatttct agtcatattc
     3001 tttcagaggt agaacaaatt gcggacacta ttggaattat tcaagatggt aaacttatta
     3061 aagaaatttc tatgtcagat atacacaaat atcaaacaga ttatgttgaa gtagatgtag
     3121 ataatgtgga gcttgctgga tacttgttag aaaaagaatt tggaataaaa gactttaaaa
     3181 ttacttcgga aagttgtatt gaaatatatg atttacgaaa agatgtaaaa gaaatatcca
     3241 aaatatttat tcaaaatcaa attggtataa atagcattgg acgtaaacaa agttctttag
     3301 aagattactt cttccaaact acaggtacag gaggtaaaga aacactatga tacatttaat
     3361 gaaactagag ctaaaaaaag ttgggctaaa aagatatttt ttattttcaa tgctggggat
     3421 tttcttatgt atgttttttg tctttactgg attgaatgat aaatctactt caatatatga
     3481 ttatgatgtt acatttagga caatcagttt atttttctgt ttttactata ttatattatt
     3541 tgctgtgttg aatgtggcat atataataaa tgaatataca aataaaacaa tcttgatttt
     3601 attctcttat ccacttgacc gtaaaaaact tattttatca aagttacttt tgattacgac
     3661 tcttatgata tcatcaatga tagttggata tatctgttgt tttacattcg ttgttgtgtt
     3721 ggataagtat tttaatttag ttattggaga ttttcatatt tcaattttat cttattggat
     3781 accaggagca attaaaagta ttattatatt ttgctcatta ggtatatgga cattcgttgt
     3841 tggaatgata agaaaatcaa tacctgttac ccttgtcagt tcaattattt ttatcaattt
     3901 aagacaattt attttagctg gtacaaatac aactcaagac agtttgattt ctgtttttat
     3961 tatagttggt ataactgtga ttggtatata ttatatacta aatcataaag ttactgaaat
     4021 tgactagata ctgtggctag gaaggtagtt attaattgta tcttcttggg ataataatga
     4081 tttgctatta tatcagctat tattaatatt attagagttt taataatatt gcacaaccat
     4141 ttgaagttga gccaaagctt gatgaattaa ttgaatggta ctatagtcag agtgaagtat
     4201 ctattaaagt tatagctgaa tttcattata gatttgaatt gatacatcca tttcaagatg
     4261 gtaatggtcg cataggtaga tttgttatgc taaaacagat gttagaaaat aatcttccta
     4321 taaaaattgt atcttgggat agtgaggact tgtatagaaa ctccttaaat tcttgtagtt
     4381 taggtaatta tgtcccttta attgaatatt tatcaagttt agaagatttt agagaagtat
     4441 ataaaatgtt atggaaataa ttgtttgtag caaaaaaagt ttttatataa atatattttt
     4501 aactatggta tttaacgctg atttcttagt tattcttagc ttggactata cccactattt
     4561 taactatata aaatagtgga tatagtttaa ctatctcttt ataactattt ttatgtatct
     4621 tagttgtttc ttaatatggg gacagttcat aaaactaaaa gtgattttta attttatcat
     4681 ttgtaattta ccattacatc agaaaaatgc ttatcattag aattttcata aaaggtacct
     4741 atttttttca attaactgaa tcaattctat tagtcttaat ctttcatatt tcaaattgat
     4801 aatataagaa aataaaaaat aaaaaatttt aaatttttta tttattgcga aaaaaataaa
     4861 aaggtgctat actttttgta acatattatt caaattatta agagatagta aacatttata
     4921 gtttaatttg tagtacacta gcatgatttg cag
//
LOCUS       DAEORZ010000215         4823 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEORZ010000215 DAEORZ010000000
VERSION     DAEORZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.221"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            135..1481
                     /locus_tag="KRH39_003600"
     CDS             135..1481
                     /locus_tag="KRH39_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8513468.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     gene            1559..2953
                     /locus_tag="KRH39_003601"
     CDS             1559..2953
                     /locus_tag="KRH39_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBG8513469.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKKLHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3075..3749)
                     /gene="fsa"
                     /locus_tag="KRH39_003602"
     CDS             complement(3075..3749)
                     /gene="fsa"
                     /locus_tag="KRH39_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG8513470.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEETLEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            4023..4787
                     /locus_tag="KRH39_003603"
     CDS             4023..4787
                     /locus_tag="KRH39_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG8513471.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
BASE COUNT         1653 a          623 c          672 g         1875 t
ORIGIN      
        1 aaattataat tatattcttt acattttaaa aaaatatatg atattttgat acaatttatt
       61 tatagtttag atataaagtt tatgttaaga ttatactata ataaattaat aattttttta
      121 tttggaggaa taaggtgaaa gatttaacta caggtcatga gggaaagtcc atattctttt
      181 ttgccatgcc tatgcttatt ggtagtttat ttcaacaact atacaataca gctgacagta
      241 taatagttgg tagatttatt ggaaaagaag caatggcggc agtctctgga gctaacccta
      301 taatgttttt attggtggca gcgcttatgg gtgttagcct tggattttct attttagtgt
      361 cacaatttta tggctcagga gatttaaaaa aggtaaaagc tactattgat actacatata
      421 tacttttatt tattggttct atattaataa gtattcttgg aatagtattt ggtggcccca
      481 tgcttaagct tatgaataca ccagaatccg tttttgctca atcaaaattg tatcttacta
      541 taatatttag tggaatatta ttttctgctg gatataattc agtaagtgca atacttagag
      601 gacttggaga ttcagtaact ccactttatt ttctgataat agccacaatt ctaaatatag
      661 ttttagactt aacttttatt gtagtgctaa gaatgggtgt tgagggcgtt gcacttgcca
      721 ctataatggc tcaggcagta tcatttataa taagtataat ttatttaaat aaaaaacatg
      781 aagtgcttaa atttaaaatc aagggaattg tatatgataa caaaatattt aaagatggac
      841 ttagattagg acttccaagt ggagttcaac aaatgttgtt ttcaattgga aatatggctc
      901 ttcaattttt agttaatagt tatggtacat ctgctatggc agcttttggt gctggtctta
      961 ggatagaaaa ttttataagc ttacctatta tgaatcttgg ttctgctgtt tctacttttg
     1021 ttgctcaaaa cataggtgct ggagaaaatg aaagagttaa aaaaggcatt cgagaatcta
     1081 taaaaatgac cttagtttta gcagttacag ttattgcttt aattttgcta tttagggaaa
     1141 atctaattgc tctattcaat actgataaag atgtcattaa aattggctct tcttacttat
     1201 ttataatagg accattcttc ttatttattg gaacttcttt tgtactttca agtgcaatga
     1261 aaggtgctgg tgattccatg tttgcactta taagctcaat agtatcttta tggcttggaa
     1321 gacttccagc ttcttatatg ctttctaaat tttttggaac agacggaatt tggatgggta
     1381 ttccttttgg ttggacttta ggtcttattg ttacagttat ctactataag aaaggacatt
     1441 ggaaaactaa agctattgta aatcatagaa tcaatgaata atctttttat aaaatgtatt
     1501 cacattcatt gtattcacct ccattatgat ataataaatt atggaggtga atataaaaat
     1561 gggtattatt cttaagccaa aacaatttta tatgactgtt atagctacca taattctcta
     1621 cttctctctt aattacagtt tcaacataaa attttctata tgcttatctt ttttaactat
     1681 cttgatatgg gctgtagact cagttgaaaa aacttttgtt gcattgtttt ttgtaattct
     1741 agcgtttata tttaacattg caccaataaa ggttatttta cagtttttat ttacagaaaa
     1801 tttttacata ataattcttg catacataat tactaatgca gttacaaaaa caggtgtcgc
     1861 aaaaattatt tctgagaagt taattttaaa tactgtaaat acccctaaaa aaatgattct
     1921 tttatcgtat attttaggta tacttttaat attttttatc ccacaaccat ttccaagggt
     1981 tattcttgta tctgcttttt acaaggagtt tttaaaagaa caacagataa ctgaagattc
     2041 aaaatctata cttttattta gtatatttac agcatctacc tttacatcaa tgttttttgt
     2101 aaatggagac actcttctaa attatgttgt actagaactt ggaaattgta atataaattg
     2161 gggtcaatgg gctttttata tgtctgttcc aactataatt acttgtttta taacttattt
     2221 tttatttatc tttgtcttta aaaaaaaatt gcattcaata aaatttattt ctaatgaaaa
     2281 tcttgagggt aataaacttc atttttcata ttctaattta aaaaaagaat ttaatttgct
     2341 aaacaaagag caaaaacacg tatttttatg cctaggaata atgtttttta tgtttttaac
     2401 tcagttcata cataacataa acacattaat tataatgtca atttgtgtac ttcttttatt
     2461 actaagaaaa atagttggat ttagtacttt aaaagaaata aattggaagg tccttatttt
     2521 ttttatagca gcattttcaa ttggaggagt gttaaagtac tctggagttg tagatataat
     2581 gggaaattat ttgataaagt ttataccaaa ctcttcagaa attatttcaa tattgttttt
     2641 gataactctc acaataatct tgaatatatg tcttggaagt gctgtcacta catcatctgt
     2701 agtaatacca ttacttggaa gtttacatat actaaaggaa aatagtgtta ctttatgtct
     2761 atttgtctac attatagtaa gcatacagta catattacca tttcatcatg ctacaataat
     2821 ggttggtcat ggtgaaaatc tgtataatag caaggttatt ttcaaatacg gtttaacttt
     2881 gattccttta acctacttta taataatatg tataacattc ccttggtgga aatttatagg
     2941 actagaaatc taaaaaagat gaaaatatga attaacttaa gataataacg aaattattaa
     3001 aaagaaaatt catattttca tctttttata taagttcttg taaaattgga caaaactact
     3061 cttttaccat aatactaaat attatttgtt ttacttcctt ttccaaaagt attctcccaa
     3121 tcctctataa acttatctac actccaatca gttaatggat gattcatcat agatttacaa
     3181 atatcatatg gtactgtaac actatgcacc ccatgttgca ttaattctat aacttgttgt
     3241 gcatttttaa aactggctgc aagtactttt gtatccattt tataagtatc tattattttt
     3301 ataagttctg aaaccatagc tactccatca ccagatatat tatcaagcct atttacataa
     3361 ggtgcaatat aatttgctcc agcctttgca gctaaaaaac cttgatgagc agtgaatatt
     3421 gcagtagcag ttatttttac accttttctg tgaaggtctt ttatagctct taaaccatct
     3481 tgtgttacag gtatttttac atatatattt tttctcaaac tacttatata taatgcttct
     3541 tctaatgttt cttcatactt tgtacttaac acttgagcat gtataggcat atcctcacct
     3601 ataatctcac caatttcatt tataagttgc ttaaaattct ttttttcttt tgaaacaata
     3661 cttggatttg tagttacacc atctattggt aaacaagtac ataactcctt aatctcctcc
     3721 acattacctg tatctaagat tagttgcatt aaaatatccc cctttaatct ttctttattc
     3781 ttattatata ttgatttggt attattttca acattttgct tattgttttt tattttttat
     3841 tattgtttta cttttccttc ttagttttat aattttttta tttactaaat tctaaatata
     3901 tagttagaaa aaacatactt tttatattaa attaattttg tatttcatgt taaaatagtt
     3961 gatatattgt attttatctt ataatattta ctataatact atattgcgaa aagaggtaga
     4021 aaatgtttgc agaagaaaga atccaagcta tattaaatat cctaaaaaaa gaaggtagag
     4081 ttactgtcaa agagctcagt tctaaattta atgttacaga agattgtata agaaaagatt
     4141 taaaaaatat agaaaagatt tcttcaataa gaagaattta tggaggagct gtactagcta
     4201 gagaaacact agagaatcag gatacaaaag atagaaaaga aattaatatt ccaacaaaga
     4261 aaataatagc agaaaaagca tttaatttaa ttgaagatag agaaactata tttttagata
     4321 tctctacaat taacatcttg cttgcaaagt tgttagctga aagtaataaa aaagtaacac
     4381 ttgttacaaa tatgctggat atactaaatg tagttacttc aaaaccaaat aatttaaata
     4441 taatttccac tggaggttta ttaaatctca gtttagacgg ttttgtcggt actccaacta
     4501 tagagtttat atcgaaatat aagtttgata agacttttat gggaagttgt ggagtagatg
     4561 tattcaactc ttcacttaca acttttgaaa ttgaagatgg tcttacgaag aaggctataa
     4621 taaattcaag taaaaaagta tttatagtta tggaagataa aaagtttaaa tttgatggaa
     4681 atttcaagtt tgctcattta gaagatatta gttctataat tacagaaaaa acgcctactc
     4741 ctgatatagt agatatttta tcagaattta atgtaaatgt tttataagtt taattatata
     4801 ttttatagga tttcttataa ata
//
LOCUS       DAEORZ010000216         4669 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEORZ010000216 DAEORZ010000000
VERSION     DAEORZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.222"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(772..990)
                     /locus_tag="KRH39_003604"
     CDS             complement(772..990)
                     /locus_tag="KRH39_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8513472.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(991..1398)
                     /locus_tag="KRH39_003605"
     CDS             complement(991..1398)
                     /locus_tag="KRH39_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513473.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(1484..1972)
                     /locus_tag="KRH39_003606"
     CDS             complement(1484..1972)
                     /locus_tag="KRH39_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBG8513474.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     IAVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(2177..3286)
                     /locus_tag="KRH39_003607"
     CDS             complement(2177..3286)
                     /locus_tag="KRH39_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG8513475.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIAKGKREEKF
                     NIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(3659..3829)
                     /locus_tag="KRH39_003608"
     CDS             complement(3659..3829)
                     /locus_tag="KRH39_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513476.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(4001..4444)
                     /locus_tag="KRH39_003609"
     CDS             complement(4001..4444)
                     /locus_tag="KRH39_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8513477.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMHALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         1571 a          748 c          407 g         1943 t
ORIGIN      
        1 tttggaagta tatttggaag taatttcata tttatttaac ttaatttttt tgtgataaaa
       61 taattctaat tttcatatag atattattat aaattttcta gtttatggag tcattcattc
      121 tataattcac aaatatgtat tttttaatct attaaagtat tcattttctg gcttataagc
      181 atggacttct tatctataag cttggttttt catctaataa gttatttatt ctttaatcta
      241 tgagcttgat ttttcatcta taagtcattt attttttaat ctataagttt agttcttcat
      301 ctataaataa gtcatttatt ttttaatcta taagtttagt tcttcatcta taaataagtc
      361 attttttaat ctataagctt ggtttttacc tataattaat tcattctcta ctctataagc
      421 tttcttttta cctataatta attcattctc tactctataa gctttctttt tatttataag
      481 tcatttattc tctaatctat aagcttactt cttaatctat aaatcattca ttttttaatc
      541 tattagcttg ttttttacct ataattaatt cattctctaa tctataagct ttctttttca
      601 tctataaatc attcattctt taatccatta gcttggtttt tcatctataa gtcatttatt
      661 ctctaatcta taagcttggt tttttaccta taattaattc attctctaat acataaactt
      721 gaatttttta tctattaatt cattctctaa tgcataagta atacatgaaa tttaaatatt
      781 attttcttca ataaattcaa acaactcttc tacagttgta tcaaatacct tagcaatatc
      841 cattgccagt tttaaagaag gattgtagcg accattttct aaatgtacaa tagtttcacg
      901 tcttactcct actgactttg caagttcacc ttgtgtcata aggttctttt ctctatagga
      961 tttaattttt gtctttagta atactgacac ctaattgtat atcccctttc tgtcaaaata
     1021 tataaataga attaacttca acaaggattg aataaacaaa atgactaata aagacattcc
     1081 tatatttaaa tttgacacat ttatgtccaa agttacaaga aatatagata ctatagctac
     1141 agaaaaaaac aatactttta ttgcaatatc attaactctt gatagtatac attttgatgc
     1201 ctcatcaatt aaatcatcat actttttatt atagacccac tgagctccca tagctaatac
     1261 ccaaataatt ataggaagat attcaatact acttccttta aaaaaatact tatttactaa
     1321 tgtagctcca atacaataag aaattcccaa tatttctgaa ataagtgttg atttaatacc
     1381 agtttgttgt ttagacatat aaaatccccc ttaaaaagtt atgtatttat aacacctctt
     1441 gttataaata tataactttt ttgaataaat ttcaataaaa atattatttt gcaacaaatt
     1501 cttttattaa ttttacatta gatagaggca ctttagatgt cactccttgc aatgtcaaat
     1561 cttctatatt aacttcattt aattttgaac acagttctaa gtcaaatatt ctttcccaac
     1621 ttaccttctt ttcaatttgt tcatcttcat aaagctctac agcaatatcc attaaaggta
     1681 aatgccataa atcaaattct gataataaca cattgtcttc acttatttct atttcaagta
     1741 aaacaccttt ttttcctttt tctaaataac cactacatct taaatcaggt ctcttcgtcc
     1801 aaacccatat catactatca tcaaattcat atattctatt tgtcatttga ctatacatcc
     1861 atttgtatgg aatatcaaag tcttctaata taaaagaata atcatttgat agatatttat
     1921 tgtttttatt gttgttccaa aattctaaag tctgtatagt ataaaatttc atatataaat
     1981 caaccccttt gtttttctat aagaaggaga ttgttcaaac ttttattttg aattatctcc
     2041 tttatttata cacctctttg tacctattat ttatatatct ctttatatct accatattta
     2101 aataacaaag ttttatatta atttaaattt aaaagaggaa gctttgtatt tatctagtgc
     2161 catctcaatc atagtattag ttttgattat agactttttc aacctctgat ttattcaaat
     2221 catctataat atcgcatatt tcactcaaat tgtatccttt attttttaat tttatgatga
     2281 tattaaattt ctcttctctt ttcccttttg caataccttt ttcgatacct ttttcaatac
     2341 cttcttgaaa aatttcttct ttaaaatttc tacttttatt cataatatac gcacctctct
     2401 ttaaatttaa taaatagtcc tcattactac ctaattcttt taatctatca agtgctcttt
     2461 taataacata atccgtttta gatagtattc taatatctat atgagaagga ttatcaataa
     2521 gtttacccca tattagaact ctgtttgcta tacaactgga tttacgaatt tttggaagtt
     2581 ctataaaata taatgcagtt ttactagaat aaatatctgg atgcccgtcc attgttaatc
     2641 tcaggcaatt tacataatct tctatgtgat tatattttac atgatttagt atatgtattc
     2701 caacagtttt tccaatttct ttatactctt taccttcttc taactttcta ccatgtacac
     2761 gactaagata atattcagct ctttccaaat aatcctcttc aagagctctt tgcatctcaa
     2821 tatttatatg gttacgttct tcatcttcag ccaaaatatc aagtctaggt cttttctcac
     2881 caatgtaatt aattgttggg tcggtttcaa ccttaattat tctttttatt tgagtgaact
     2941 gtttgatatt cctattagca tatggagcat ctttgaggat atcatttata aaatttgcaa
     3001 gtaaatcgta atcattattt aaagtaaaca atgatttaaa tacctcatca tactcaggct
     3061 tagcataccc atcttcagtt acaacttctc caacattttc actagtcaat atttcttttg
     3121 gaattaaatc cttgatttta tctaattttt cattagaaaa atcacctcta ctaagtatgt
     3181 catttaattt tttggtccat tctctgtcct tttcttcaat tatagttcgt ttatactctt
     3241 ttgatgtttt aataatctta gatttttctt ctaatttttc tttcatatca acacctcttt
     3301 tttattatta cccatttctt cataccaaag tctgaacttc taagtaaaat ttgcattatt
     3361 gaaataagaa ccttataaac ttatatatca ttactaaagt taagacttta aagagaagca
     3421 cttagattac aatagatttt agatagcttt ctttttaaaa ctattgttga taaatgaatg
     3481 aatataaggg tatgtattaa aatagagata ctcatctttt tcattgttta agtaatgaaa
     3541 atatatttta tttaatacaa aaaagagtgc cttaaaacta aaaagttcac ttcgagacac
     3601 tcacttacgt tttgtttctt aaacttcaca atattgcata cttatttatt aatttaatct
     3661 atgcatactt aacccaataa taaagtacat atatccaact cattaagcca tgaaaaatag
     3721 cccatggaat agatttccat gcagtataag aaataatcat tgctaaacaa gaaccaaaag
     3781 aaataccctt tttaacagtt gattttatag tattattatt tgaatccata attaatcctc
     3841 caaaattcaa aataaacaag ttattatata tcttatttga atataataca atataacagc
     3901 ttctaaataa attatactct tattatacat aatttataaa ttataattcc gataattata
     3961 tgaattaatt aattatatta acctaaaaat attatcctat ctaaaagtta tatgatagat
     4021 acaacatgtc aacacattgt aattctcctt caaagattgg ttctggataa ttatctacaa
     4081 aaaagttttt aattttatgt gaataaacaa atccaaactt ctcataaaaa ggaactccac
     4141 tttcagatgt accaacaacc attgatgaat attttttact ataaacatct aaaagatgat
     4201 aaatcatctt tgaggcataa cccatatttt gaaaatgctc atatgtagca atgtttttta
     4261 attcacatat ttctttggaa atttcattta caactgctat acaagtaagt tctttattat
     4321 catacaatgc gtgcatagtt cctttattga gatatttatc aatcatttct tcacatgggt
     4381 cggctaacat taaaaggtca atttgacttt ttttatttat ttctatagtt tttatttcta
     4441 tcataagttc tccaccctca ctttattcaa accttaattt agcaaacttc ttacagtact
     4501 tatttagttc attttgatat atatttttac ttttttaaaa tatagtgata attccctaga
     4561 atattaatat taataataaa ttataatatc agctctagca gttacctaga atatcaatac
     4621 taatattcat ctataatatc agctctagca attacctaaa atatcaata
//
LOCUS       DAEORZ010000217         4592 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEORZ010000217 DAEORZ010000000
VERSION     DAEORZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.223"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            50..1219
                     /locus_tag="KRH39_003610"
     CDS             50..1219
                     /locus_tag="KRH39_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBG8513478.1"
                     /translation="MNELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(1382..1924)
                     /locus_tag="KRH39_003611"
     CDS             complement(1382..1924)
                     /locus_tag="KRH39_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBG8513479.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(2072..2356)
                     /locus_tag="KRH39_003612"
     CDS             complement(2072..2356)
                     /locus_tag="KRH39_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG8513480.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(2419..3183)
                     /locus_tag="KRH39_003613"
     CDS             complement(2419..3183)
                     /locus_tag="KRH39_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG8513481.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(3465..3731)
                     /locus_tag="KRH39_003614"
     CDS             complement(3465..3731)
                     /locus_tag="KRH39_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG8513482.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(3773..4582)
                     /locus_tag="KRH39_003615"
     CDS             complement(3773..4582)
                     /locus_tag="KRH39_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8513483.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIEELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         1556 a          643 c          637 g         1756 t
ORIGIN      
        1 tgaagcagga accctgtgac tttagtcatg ggaggttcag ggtagaaaga taaatgaact
       61 ttgtaaagat gaagttgtag agttttgcaa tagaataaaa ggtaaagtgg aaagtgttgc
      121 aataagtgga gtatttgctc cagtaaataa acaacaagaa gaagaagttg caaaatgggt
      181 tagagaaatt ttaggggata ttcctatatc tttatcaaat gaaataggta atataggtat
      241 attagaaaga gaaaatggag ctattttaaa ttctgcattg tctcaagtag gtaaagctgt
      301 atctttagga tttgaaaaag cacttaaaga cttaggtata gatgctcaaa tatatttcag
      361 tcaaaatgat ggaacattaa tgaatttaga gtatacaatg aaatacccaa tttttacaat
      421 tggatgtgga ataacaaatt ctataagagg agcttccttc ttatcaaaag taaaaaatgc
      481 aatagtatta gatgttggtg gaactacaag cgacttgggt gtactatata atggattccc
      541 tagagaatca tctattccag ttacaattgg aggagtacat acaaacttta gaatgccaga
      601 tgttttaact attggtcttg ctggtggtac atgtgtaaaa ggcgaaaaag ataatataaa
      661 gataggtcct gaaagtgtgg gatatagaat aactgaagat gcaataatat ttggtggaga
      721 cactctaact ttatcagatg ttgttacaaa attagatatg gcgtttgaag atagaaaaag
      781 caatgttgaa aacttagata aagagtattg taatgaggta tataagaagg ttattttaaa
      841 attagaagat gctgtagacc aaatgaagac tagtcaagat gatgttgaat tggttttaac
      901 tggtggagga agtataattg ttcctgaaaa actaaaaggc ataagcaatg ttataagacc
      961 tcctcattat actgctgcaa atgctattgg tgcagcttta ggacaaataa gtggagatgt
     1021 agaaaaagta tacagtttag ataaaatgtc gagagaagat gccatagaag atgcaaaaaa
     1081 agaggctgta aataaagctc taaaagctgg tgctaaagaa gatactatag aagtattaaa
     1141 tatagaagat gttccacttg cataccttcc tggtaatgca cttcttataa aagtaaaagt
     1201 tgttggtgat ttaatttaaa gaataaatat aagtatttcc agaattatat aaatatttct
     1261 ggactcattt ttaaacttat tttatcaata aaaacagcat aacttttgac tatattctta
     1321 taagtttccg aacagccgat atatacctat tttttgaggt ttatcggctg tttgttatct
     1381 tttatcgcat tccaactatt tttcttaccg tatttgctgt acgaattgta atcttatcac
     1441 ttacatttga actggcagtc tttgaccacc aatttgtctt tttataatct ttacgactaa
     1501 aagaccaaaa gatagcattt ttataatgat atactttttc atactcttct ttaggatttc
     1561 caacctcatc ataaaactcg tcataagata agtcaggaaa cataaaaata agattatcat
     1621 atatttcttt gttgttatct ccccaccaat taggagcatt atttagtata tcttttaact
     1681 cttcttgtga aataataaaa actggtattt ccaattcaaa ctgttttttt atcattaatc
     1741 ttattgtatt tgagataacc ttgctgtcat ctacatcact taaaaagaca acattaccac
     1801 tattaaggta tgtagaaact tctgaataac caagttctac aaagcttgct tttaattcag
     1861 acataattat cttgttctta ccacttatat tgatacctct taataatgcg atataacttt
     1921 tcatataata atttcacctc taaacattca catttttcta tgtagtacaa actggaattt
     1981 gcatctttta taattctttc aaaacttctt catatgtaat ttttttaata taaaaaaatc
     2041 ggtgcttaat ttgtaccgat ttttgttttt cttagtaaag aactcgtttt tttgcttctg
     2101 ctatagctct agcagcactt tttgcttgca ttcttttttc ttcttctttt ttaaattcat
     2161 ttattgtttc atttagattc ttatcatgtt catttttttc tttatcttcg ttttctctgt
     2221 atactaatat ataatctatt attgattcgt ttattatttg agatatactt ttattttcat
     2281 taattcctat tctcttaact tccttaagta gagtctcatc aatatgaacc gttgtttgct
     2341 ttgtattttt tgccacctta atcacctctt aaaaaaaatt ctaacattaa aatttttttt
     2401 agtcaataaa ttttaatatt aaaattcttt aaatgtttta tttgattttc taaaattttc
     2461 tattgtattt atcatcttat tttttatatt ttcacctagt acagaagact ttagtctagg
     2521 attttcgata tggtcataaa ttggttctaa aaattcgact tcgacaggaa cagagttaat
     2581 tttaccaata ggttggtatc cttcataagt atcttttgaa ttttttatta ctagtggaac
     2641 aatagggcat tttgctttat atgctatctt taaagctcca cttctaaact ccgaaaccaa
     2701 tgaatttggt tctttaatcc atgttaaatc tccttctgga aacactgcca tactttgacc
     2761 tgtaagtata ttttgagagg cttgggctat actctttata ccctcacgat tgttttttct
     2821 gtttacatat acacatctta gtaattttgc ccattcttta aatataggta tatttctcca
     2881 aactggttca tctgcaatta cacatccaat tggtctttct acacttgctg ttagtatgaa
     2941 gctgtcaagc atactagaat gatttacaac aaatagaagt ggttcttttg gcagtgtttc
     3001 cttaccaatt atattaagct ctatgttcac tatatctaaa gattttttag cttgtttttg
     3061 cataaattca aatttttcct ttaagctaaa tttatcagga ttctttttaa ttttaataag
     3121 cctaggtata gaagatgtaa aacctataag ttgatatgta gcaaatttta agtaattatt
     3181 cataaaatta tctccttaaa ataaaattat ttattactgt aaagatttta acataaaata
     3241 aaatttaagt ctattttaat tcaattttgt aacatatatt ttaaatagac agagattgag
     3301 attttatatt tccaatttta gtaatcagat tttatcaata aattactatt ctattaattt
     3361 aattaaataa cctttaaatt taattataaa aactgttgca gaaatttcta caacagttta
     3421 aaaaatttac cttacaaaaa cttattttat aacaagagag tatattataa ggctagtaca
     3481 gcccattttt tgcactgaga aattcttttg ccatcatcac cttgttcttt gtcatatgca
     3541 aattgattaa gcatttcaca tggaaatgta tcacatagac cacaatgtgt aaggttttta
     3601 tcttcacaac aagattttac aggacattgt tcaccccaaa atggtttctc aatctctgta
     3661 caacctttac aattgacttt ttccttgtat ccacattcat aacataaaat tccacatctt
     3721 gattctatca ttttaagtca ctccttaaat ataaatttta tatgattgta tatcaaaatt
     3781 tatacttttc attgtaaaaa tcggacaaat tttttatata ggttgttgga ttaacaccac
     3841 aaatagattt aaaatcattt ataaaatgag attggtcgta ataaccaata tcttgtgcta
     3901 aagttgcaaa tggtatttta ttattttgta tttcttgtaa tacaaggttt atacgaagta
     3961 atctaaggta tgatttaaca ctcataccaa gagaattatt aaaaattcta tttaaatgtc
     4021 gttcactata acaactaatt tgtgaaatgt ttttaacaga cataatagag ttttgttttc
     4081 tagcattttc aagaattggt atagtcatat ctactatatt tgatttcaat aagtatgata
     4141 agaataacat atctaattgt tctactaatt cttctatagt acttgttctt tcaaaaatgg
     4201 aattaacctc taagctaaat aatgtgttaa aatcttcaag tgaaattttt aaatctgtag
     4261 attctctttg agaaagacct gtcaaataat atactccacc tgggcgaaac tcaacgaata
     4321 ctctaaaaag tacattttct atatcatttt ttacaatagt agtttttgtt gtggctcccc
     4381 agaatgcgct ttctatacct tttttgtcaa atttaaatat catacatcca ctagcatcag
     4441 gtattaatgt aagattttct ttaatacttg tatctggaat agatattgta tagtgtgcta
     4501 tatactgtct taacaattta tgtggcaaaa tatatgtata ctgtttgcag gtatttaaaa
     4561 tgttattgat tattttattc ataagtatat cc
//
LOCUS       DAEORZ010000218         4514 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEORZ010000218 DAEORZ010000000
VERSION     DAEORZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.224"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            3..689
                     /locus_tag="KRH39_003616"
     CDS             3..689
                     /locus_tag="KRH39_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bclB domain-containing protein"
                     /protein_id="HBG8513484.1"
                     /translation="MGATGPTGADGATGATGATGPTGADGLVGPTGATGATGLAGATG
                     PTGATGPTGPTGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVID
                     LTNAAGTLTNFAFSMPRDGTITSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFT
                     AVPGATVTLAPPLTGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVA
                     GYASAGIAIN"
     gene            861..1622
                     /locus_tag="KRH39_003617"
     CDS             861..1622
                     /locus_tag="KRH39_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG8513485.1"
                     /translation="MKNKIETLMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLKNGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHIAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            1931..2209
                     /locus_tag="KRH39_003618"
     CDS             1931..2209
                     /locus_tag="KRH39_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513486.1"
                     /translation="MSYKKAIHVLPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            2502..3077
                     /locus_tag="KRH39_003619"
     CDS             2502..3077
                     /locus_tag="KRH39_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBG8513487.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            complement(3183..3821)
                     /locus_tag="KRH39_003620"
     CDS             complement(3183..3821)
                     /locus_tag="KRH39_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBG8513488.1"
                     /translation="MIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGYASDIRMQ
                     KTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLNKESKLDK
                     AVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRAKKDNRFL
                     EALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLDK"
     gene            complement(3848..4477)
                     /locus_tag="KRH39_003621"
     CDS             complement(3848..4477)
                     /locus_tag="KRH39_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBG8513489.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMI
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         1676 a          578 c          744 g         1516 t
ORIGIN      
        1 caataggagc aacaggtcca acaggagcag atggagcaac aggggcaaca ggagcaacag
       61 gtccaacagg ggcagatgga ttagtaggtc caacaggagc aacaggagca acaggattag
      121 caggagcaac aggtccaacc ggagcaacag gtccaacagg cccaacaggt gctagtgcaa
      181 taataccttt tgcatcaggt ataccactat cacttacaac tatagctgga ggattagtag
      241 gtacacctgg atttgttgga tttggtagtt cagctccagg attaagtata gttggtggag
      301 taatagacct tacaaacgca gcaggaacat tgactaactt tgcattttca atgccaagag
      361 atggaacaat aacatctatt tcagcatact tcagtacaac agcagcactt tcacttgttg
      421 gttcaacaat tacaattaca gcaacacttt accaatctac tgcaccaaat aactcattta
      481 cagctgtacc aggagcgaca gttacactag ctccaccact tacaggtata ttatcagttg
      541 gttcaatttc tagtggaatt gtaacaggat taaatatagc agcaacagca gaaactcgat
      601 tcttactagt atttactgca acagcgtcag gtctttcatt agttaatact gtagcaggat
      661 atgcaagtgc aggaattgca ataaattaga ttaaataatt aaaaaaggct tctagtatct
      721 tattgatatt agaagctttt ttaagactga ttgataaaca tagttttaat attaattagt
      781 acacatatta taaaatacta ttatataata taaaataata acattaaatt ggaaatatag
      841 aattatctag gggtgatact atgaaaaata agattgaaac attaatgaac aactttgtaa
      901 aaaattatca taaatcaaat aatataacta caaagtggca agaactttta tttgcttatg
      961 cctctgctga agatgatatg tttcagaagc taaaaagtgt tgtaggaaca tctcatgcta
     1021 tgccaaaaga ttttctgaaa aatgggaaaa ctataattac atattttata ccttttgatg
     1081 aaagtgtaaa taatagtaat attggagggt ttgaatgttc tgaaatatgg gcaagagcgt
     1141 acatagaaac taatatgttg atttcaaatt taaatgattt tttatgtgaa gagctagaga
     1201 agataggggc taattcatat gctattcctg ggtcgtataa ttttgacagt gaaaaattgg
     1261 tcagtgattg gtcacaaaga catatagcat tcatagcagg tcttggaact tttggtttaa
     1321 ataatatgct tattacagaa aaagggtgtt gtggaagaat aggaagcata atcacaagtt
     1381 tagaattaga gccaactaaa agaccagaaa atgaatactg cttgtataaa cataataata
     1441 gttgcaaaaa atgtgtcaat aaatgtgtaa aagaagcttt aaaagaagat ggatttaata
     1501 gaaataagtg ttatgaaatg tgtatgataa atgaaaaaaa atacacttat ataggtggat
     1561 gtgatatttg tggaaagtgt atagttggag ttccttgttc agtaataaac ccatcaagat
     1621 aatggattta agttgactaa taatttaaaa tatagtttat aatgaaaaaa ttttagttat
     1681 caaaataaat ataatataaa aactaaataa atgtgtttaa aaaatggata aataatttgt
     1741 gttgattgac aaccagttat actgttgata gacttatatg tatcgctgtg atatataagg
     1801 agggttgaga ttttatgttt gatttaatct ttatttttgc atagcaaggg ctattgcaac
     1861 tatgaaattt aatatataaa tagccctttg ctattcctaa tgaaaaatta ctatttaata
     1921 ggagagcaaa atgagctata aaaaagcaat acatgtatta ccagaagaat tacttgaatt
     1981 aatacaggaa tatgtagatg gtgaatgtat ctatattcct agaaaatcta ataataaaaa
     2041 agaatgggga agtaatacac ctactcgtga agagttgtca attagaaata tgcagattta
     2101 cgaagactat caagttggat atgatttgca atatttatcg gaaaagtatt atttatcttt
     2161 gaaaagtatc caaagaattg tattacaaga aaaaagaaaa gatatctaag gatgagccag
     2221 tatagtttta taattatact ggctcatttt cttttgataa aagtgtttga ggtatagttt
     2281 cttttaagtg gatgatataa tttaagtaat ttcaataact tatacagtag ctaacaaagc
     2341 tggttgtaaa gtatctaata agaagtagta gctttttata tgtttgaaat taaaatttat
     2401 taaaattagg aggtcttgtt ttgaaaaaaa tacttgatta tattccacct tttcataact
     2461 ttctattaat ttagtaatct tatagaaagg aaattgcatt tatgaaaaaa attacttggg
     2521 aggtggaata tatctcccag ttacctgtaa ttaggtattg taaaaaatgt ggaaaaaaga
     2581 ctgaatatat ttgttcaggt ttatttaggg ttaatgctca acataaatat ttggatatat
     2641 ggttaatata caagtgttca aattgtgatt caacatggaa ctcaactata tattctcgtg
     2701 ttaatccaaa aagcttaaat ccagaaatac tagaacaatt ccatacaaac gattataatc
     2761 ttgttaagca atatgctatg aatatggaac ttttacatag aaatggagca gaagttggta
     2821 ttccagagta taaaatagta ggacaagaaa ttaatattaa tgaccttaca caagttcata
     2881 ttattagtaa atatccatgt cagttaaagg tatcaacatt attacgtgag aaactaggtt
     2941 tatctaggaa ggtatttgac gaaatgttgg attgtgggat tattagaagt aactcaggag
     3001 ttgatttaaa gaaaggtaaa ttaaattatg aaattatgtt agatataggc aaggagaagc
     3061 aaaagtcttc aaattgatta gaataactaa cttaatatat tttcaggaac atcatttaaa
     3121 aaaatagtga ctttaaaaaa tttaaagcca ctatttttta atatgtttta tctatataac
     3181 tattatttgt ctaggttttt taatatttcg taatagtgtg ttgtggctat ttcacttgac
     3241 tcgccttttt tacaaaaaga taatttggat agttttggaa ctacacgttc aacgacaatt
     3301 ttattaccta tcattttaga ttcaagtatc ttcaatgctt ctaaaaatct attatccttt
     3361 tttgcacgat tataaaatga taaaacgtag acataaagga ataggttata attacggaaa
     3421 ggatattcaa cttgcataaa taatgtgcca attccatagt gacaagggcc gattggtttt
     3481 ttaatagtcc aatggtcaag tagaaaatct acagctttgt caagttttga ttctttattt
     3541 agataattac taaaacggaa tgcatttaat atattaagag ttggtagtgg atttgagtat
     3601 tcagtttctg gacctcgacc aaaactaaat ttattgcaac gccacccacc atcagtatac
     3661 tgtgtatcta aaaaatgttg aaacgttttt tgcattctaa tgtcagatgc atatcccata
     3721 tgacagagta cgttagcagc atttgcagta tgacaagggt aaatacctcc atttggatat
     3781 agtttgaaac gtccatcttc tcgccatgtg ctaaatatca aaaactttat tataatatta
     3841 tttatattta taattgattt attttattta aaattgcatt tatagctgta tcaggaacaa
     3901 ctgctaaatc ttctgcattg aagtaataaa ctatattaga atcaaataat atatttgaat
     3961 ttggaggaaa ttcatcatct ccaaaccaaa gtataaatat catagatgta ttacccatat
     4021 atgtaaacct ataagctaaa tctcccatgt ttactttttc agctccaatt ttctccataa
     4081 ctaacttata tttgtctatt tgagatgtaa aaacttctga aagcctatat agagtcctgt
     4141 ttaaaaaatt tggataataa acattgcctc ctggtatttc tttaaaagtt acatatttat
     4201 tagttctagg aataccttta gcattgatta aatatctaac tatcaagatt ttaagctcaa
     4261 agttattgca aaggctacca ttactattcc aaacttctcc acttggatag tctataatat
     4321 attgtctatc aaaaaaattg agtgtgaatc tatttaaatc attattgaaa ggtatattac
     4381 ttaaattact aattgtatga gggtctaatt ctttgagttt ttttcgaaca tagtcaaatg
     4441 gaacattata attatttttt gttatctttt catccataat tttttcctcc aatttaaaca
     4501 attaaaagta tatt
//
LOCUS       DAEORZ010000219         4441 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEORZ010000219 DAEORZ010000000
VERSION     DAEORZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.225"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     misc_binding    82..344
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            420..3086
                     /locus_tag="KRH39_003622"
     CDS             420..3086
                     /locus_tag="KRH39_003622"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBG8513490.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     gene            3511..4221
                     /gene="rgaR"
                     /locus_tag="KRH39_003623"
     CDS             3511..4221
                     /gene="rgaR"
                     /locus_tag="KRH39_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBG8513491.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT         1787 a          449 c          815 g         1390 t
ORIGIN      
        1 aaatataatt aagaaaattc agaaaaaata aaaaaaataa ttgacaaaga gaataatatt
       61 tgtatataat aatatgtaat attaaagact gagaagaaga ggagtaagta aatactacta
      121 aaagagagga aaattcactg gctgtaagat tttctaagta taagatttac tgaatgtagc
      181 tttggagttt ctaaactgaa ctataagtaa gtttagacgg ttagaaccgt taaattcttt
      241 gagagtcaaa gttgataaaa taacattaac caataattta attatatgtt aatgtatttt
      301 aagctttgga attaaggtgg taacgcgagc ttttcgtcct ttttaaagag gatgaagagc
      361 tcttttttat ttctaaagat atttaaaaaa tttatttata aaatacagga ggcaggacaa
      421 tggaaaatac gaatttatca aaaacttata acccaaaaga ttttgaggct aggctatata
      481 aaaaatggat ggatgaaggg tattttaaat ctaaaccaaa tccagataaa aaaccattta
      541 caataatgat gccaccacca aatataacag ggcaattgca tatgggacac gctcttgacc
      601 atacacttca agatatatta atcagatgga aacgtatgga tggatatgaa gctttttggc
      661 ttccaggaac tgaccatgct tctatagcaa cagaagttaa agttgttgaa agaataaaaa
      721 aacaagaagg caagacaaag tatgaaatag gtagagaaga atttttaaag agagcctggg
      781 aatggaaaga tgaatttgga ggaaaaatat ctaatcagct taaacaacta ggtgattctt
      841 gtgattggga taaagaaaga tttactatgg atgaagggtg taatgaagca gttatagaat
      901 tttttgtaag cctatatgaa aaaggtcaca tatacagagg aaatagaata ataaactggt
      961 gcccagattg caaaactact ttatcagatg cagaagttga acatgaagaa cacgacggaa
     1021 acttttatca tataaagtac ccactaaaag atagtgaaga ctttttagaa atagctacaa
     1081 ctagacctga gacaatgata ggagatactg gtatagcagt aaatccagaa gatgatagat
     1141 ataaacattt aataggaaaa acagcaatac ttccattagt aggaagagaa cttccaatag
     1201 ttgcagatag ttatgttgat ttagagtttg gaactggtgc agttaagatg actccagcac
     1261 atgaccctaa tgattttgaa gtaggattaa gacataatct tgagcagtta aatactatga
     1321 atgaagatgg aactatgaat gaagtttgtg gaaaatatga aggtatggat agatttgagt
     1381 gtagaaaagc tatcgtagca gacttaaaag aacaaggtta cctaattaag ataaaagagc
     1441 ataatcataa tgtaggaact tgttatagat gccatacagt agttgagcct agattatcag
     1501 agcaatggtt tgttaagatg gaagaacttg caaaaccagc tatagatatt cttaaaaaag
     1561 gagaattaga atttgttcca gataaatttg ataaaactta tttacaatgg ctagaaaata
     1621 taagagattg gtgtatatca agacaattat ggtggggtca tcaaatacca gcttattatt
     1681 gtcaagagtg tggagaaata gtagttgcta gaaaaatgcc agataagtgt cctaaatgtg
     1741 gaagcactca ttttaaacaa gatgaagatg ccttagatac ttggttcagt tctgcattat
     1801 ggcctttctc tactctagga tggccaaata agactgaagc tttagattat tattatccaa
     1861 caagtgtact tgttacagga tacgatataa tattcttctg ggtagttaga atggcatttg
     1921 caggtatgtt ctgtatgaat gaaaagccat ttgaccatgt tttggttcat ggattagtaa
     1981 gagattctca aggtagaaaa atgagtaaat ctttaggaaa tggtatcgac ccattggaga
     2041 taatcgagca atatggagct gatgccctaa gatttacatt aacaactgga aattccccag
     2101 gaaatgatat gagattctat atggaaagag tagaatttgc tagaaacttt gcaaataaat
     2161 tgtggaatgc atcaagattt gtatttatga atattgatga agatataatt aaaaacatga
     2221 ctagagaaag tgttaaagag gacttaactc tagctgataa atggataata tcaagagcaa
     2281 ataatattgt aaaagaagca actaataaca tggataagtt tgatttagga atagcacttc
     2341 aaaagatata cgattttact tggtcagaat actgtgactg gtatattgaa atggttaaac
     2401 ctagattata tggagaagat gcaaatgcta aatctgcagc attatataca ttaacttatg
     2461 ttcttgaaaa aatattaaaa ttattacatc catacatgcc atttataaca gaggaaatat
     2521 atactcatct tcctactgtt gaaggatgta taatagtatc tgaatggcca aaatataatg
     2581 aagaagataa catggctgaa gaagaagata tgatgaactt attgatggaa ggtataagaa
     2641 gtataagaaa tgtgagagca gaaatgaatg taccaccttc taagaaagct aaattgataa
     2701 taatcccaag tgaagaaaaa atagaagcta ttgagcttgg taaagactat tttataactt
     2761 tagcatctgc ttcaaatgta gagatagcaa aagataaatc taatgttcca gaagatgcag
     2821 ttggagtagt tattgatggt gttgaaatat ttatacctct taatgaatta gttgattttg
     2881 aaaaagaaat tgagagatta tctaaagaaa agaaaaagtt agaaggcgaa ataaagagag
     2941 ttaatggaaa gcttgcaaat caaggtttct tagcaaaagc tcctgaaagt ttaattgaag
     3001 aagaaaaagc aaaaaaagaa aaatttgaag agatgattaa atctgttgag gaaagattaa
     3061 caaatttaga atctaaaata aaataaatat aagaattagt aaaaacatac aaaaagtata
     3121 tattctatac tttttgtatg tttttcttta tatatataca aatatgatgt tataattctt
     3181 tacagatgtg ttatattttt attttatgtt gtgaatatat gaattttatg tcttaaaatt
     3241 tataagcaat aaaaatttga tatattatga tgtttaatat agggtaataa atacatatta
     3301 tatttatttt actatatttt tttacataat atatctagaa aagtatagga atttatcgta
     3361 atttaagttt aaatattaaa gagaatttct ataagtagtt taatcgacaa tttaaattta
     3421 taaagaaatt taaatttgaa agctagtgag tacagtttgg aaattgaaat aaagtaaact
     3481 aaatgatatt ttagatttgg aggtgatatt atgttcaggg tagttatatg tgatgacgaa
     3541 aaaatacaaa gaagcatact aaaagagttt acagaaaaac ttttgtctga aagatgtcta
     3601 aattatcaaa tactggaatt ttcctgtgga gaagatttaa tatccaaata cccagaaaaa
     3661 attgatatta tatttttaga tatccaaatg aaagatatca atggaattga ggtggctaaa
     3721 aaaataagaa agtttgatga aaaagtagaa attatattta caacggcttt ttcagagtat
     3781 gccccccaag gttatgaggt tcgtgcttat agatatttag taaagcctat agagtatagt
     3841 aactttgcaa tgggtgtaaa tctttgtatt gataaccttc aaagaaagaa ggagcgttat
     3901 atagttttaa atagtaaaaa aggatttaat agaatactga tagattctat tttatatgtt
     3961 gaaactgtaa agagagattt agtcgtacat acaataggta gagattataa agctaatatg
     4021 agcatgaaac aagcagaaaa attactgtgt gaaaatggct tttttagatg tcatacaagt
     4081 tttattgtta atttaagaag aatagaagat cttagggata atatgattac tattaatgaa
     4141 caatttatac ctataagtaa gtatagactt aaagatttaa aagtagcatt aacaagcttg
     4201 ttatgtgata taatgtatta attttaaaaa ggtaattgaa aatatcgtaa aaaagattta
     4261 atgtcatcac agggctattg attgaaactg tcttaatata taaaatgtta tttatattaa
     4321 gacagtttta ttatgcaaaa tagtagttta tagattttta atatggttta acatgtatat
     4381 gtagagtttc cattataata gcatgattat taattagtag cttgattatc aatatactat
     4441 t
//
LOCUS       DAEORZ010000220         4431 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEORZ010000220 DAEORZ010000000
VERSION     DAEORZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.226"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            741..1235
                     /locus_tag="KRH39_003624"
     CDS             741..1235
                     /locus_tag="KRH39_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG8513492.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKSDYGSSIDIMNEQNKIDIVIKLLND
                     KSNT"
     gene            1624..1793
                     /locus_tag="KRH39_003625"
                     /pseudo
     CDS             1624..1793
                     /locus_tag="KRH39_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2270..2458)
                     /locus_tag="KRH39_003626"
     CDS             complement(2270..2458)
                     /locus_tag="KRH39_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG8513493.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            2620..2970
                     /locus_tag="KRH39_003627"
     CDS             2620..2970
                     /locus_tag="KRH39_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBG8513494.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNVELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            2972..3172
                     /locus_tag="KRH39_003628"
     CDS             2972..3172
                     /locus_tag="KRH39_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8513495.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     regulatory      3352..3441
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3495..3671
                     /locus_tag="KRH39_003629"
     CDS             3495..3671
                     /locus_tag="KRH39_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513496.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     gene            3896..4096
                     /locus_tag="KRH39_003630"
     CDS             3896..4096
                     /locus_tag="KRH39_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG8513497.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
BASE COUNT         1660 a          479 c          569 g         1723 t
ORIGIN      
        1 agaggtaagt tttaccaaat attttacata tttagtctgt ttttttaaac gtaaaataat
       61 attcatatat ataaatttgt ttaatttgca aacaatgcta ccaaacaata aatagctcta
      121 ttgtattgtt ttagttgtag aatagcacta aatcgctaga atcaaagaaa tttagtagca
      181 caaagctata taggctaatg cttataaaat aaattataag ttatgttaac aagttactaa
      241 aagcatattt tatttttgtt tttatgaaag ttttttaggt atgtaataat atatatcttt
      301 taaaatatgt attcatgtat acatattttg acttattata ctaatagttc atataattat
      361 tttgatttta tagatacaca gttagatgat tttatactag agcataaaaa aaatgtagta
      421 taatcttgtt tatatcaagt tatggtattg gtaagtaagt ataattaaat agacattatt
      481 ttctgagtcc tctttttatt ttcatctgct tttaaataat attattgaca ttgatatgac
      541 tgtagttttc tttattttaa tccattgctt atatcttttc taacaagaaa tgtcatttta
      601 aatcatttcc ataaattgta aaataatagt atatttgtca tagaaagtga tttttttgtc
      661 tatatatggt aaaatataga aagtagaaat caacaaaata aaaatttata tattattaat
      721 tttatatgga gggaaaataa atgaaaaata aaaaaggatt tactctagtg gaattattag
      781 tagtaattgc tataatagga atattggcaa tagtagcact tccagcatta tttaaaaata
      841 tagaaaaagc aaagatagct aaacttgaag ctgatataag tgcaataaaa agtgcgtctc
      901 ttagctacta tgcagatgaa tcaaaatata ctgatggagg aatgatatca tgggtaaaga
      961 aagatggaaa aataataata aatggtggct ttaaagatga cccattagca gataaaatag
     1021 aaaatttagg tatgccttat aatggttcat atctattaat gtcatctcct ggtcatgaaa
     1081 aatatctaga attaagtata cttccagaag gagaaataag caaaagtggt ctagataaat
     1141 taaaaagtga ttatggaagt tcaatagaca taatgaacga gcaaaacaaa atagatattg
     1201 taataaaact tttaaatgat aaatcgaata cttaaataca ataaatttta tttaataatt
     1261 gctgatgaaa taaacaatct tgtttggaga aatgaaaaaa gcactcctca agaatagaat
     1321 gcttagttat aactacagaa gtgtattggc atacacttct attttttatt atatcacata
     1381 atttaaaaat acctattcaa gttgttttta aattatgttg agtaatattc ttgatagttt
     1441 atacattaat gaatttcaaa aaaataaata ctctttataa taaaaagtac tttagtgtat
     1501 gttaatgtcc tatctaataa aattgtaaca tgccttatgc tttagtatga taattttcat
     1561 tttctttatt gttgcaactt atactatagt tttgctaatt tatctgcaaa cacaacttct
     1621 ttattgcatg tgtatcaacc aaaaacatca gaatacttac ttaatacata tgcaaaatcg
     1681 tatctttcat ctatagtatt cttatttgac tatataacaa aaaatgaatc gagatatgcc
     1741 ttttttataa ttgattttcc attttatcat tagatatatt ttataaattc tagcatatca
     1801 tttgttattt tctgatatgc tattgatatt ttaaatcttc tatttgtttt attattttct
     1861 acttttccaa ttatacattt ataggagtta tatttatgtg atttatgaaa aaatgctatg
     1921 catttaagtt ctaatttttt gataatacat ctaactttct tttgattaat aatgatacca
     1981 ctgtttttta atgtggcttc cctgaggcac acatcttttt taaaaactca agctcatctt
     2041 ttaaaaattt attttcatat tctaattctt gttctcttgt taatttattt tttctttagt
     2101 gtttttgttt ttattcatta tggtcttcct ttcattttgg aaaaggcatc aacaccacta
     2161 tgtaaaaatt tttattttca tgttgctata actgtgctat tgtttattcc aaaatactta
     2221 gcgatttatt gtacactata tttagttatc atcatatagt ttataacatc taacttgaac
     2281 catatagtat agaaaatttt tttattttta gttcactctc tcctaatata ttaacccatc
     2341 tttcaacttg agaatggtca taaactccat attgttttac tattaaaata tctcttcctt
     2401 ctctattcaa ataaaattta gctactttta atttgaaatt aaaatcatat ttagtcatac
     2461 ataaacatct caagcattag atttttgctc taacaattat ggtgcagtat aaatattgta
     2521 taatactgat tttctattga caaaagaata taaaagagat atatttgtat cataaagata
     2581 taaatatatc tcttttaaaa ttactaagga ggataaaaaa tgattcagaa aaaatttttt
     2641 atgggttttt gtggtttcct tggattttta agtcttagat atttttcatc tggcaatgtt
     2701 gctgacctta catatattgg attttttgct tttttctcaa actttattat agcaaaaata
     2761 aatggtgata aagcagatga aaggtatgta caagatgaaa aagcagctat ggcatttaca
     2821 ggacagcttg ctattataga attattcatt ttatggtgta ttactatagt gtctaggaat
     2881 gtagaattaa tgtgtgtttt actatcaatt acatatgcaa tcactttaaa tgtttatgct
     2941 ataaaacttt atattttaga ggaaaaataa tatgcccaaa ttcatttgta acctaaaaaa
     3001 atatagacaa ttaaaagaat taactcaaga acaattagca gaaaaagtag gagttagaag
     3061 agaaaccatt atgcgtttag aagcaggaaa gtataatcct tctttgaaat tagcaataga
     3121 tatatcaaaa atagtagata cacctattga tgaattgttt atttttgatt agtttttgca
     3181 ttattaaaaa tgtgttttaa atacaaactt aaattcaatt tttaagtcac tcctgacaga
     3241 ggggcttttt attttttcag ataatttaac gttcatatat aaatttatta atttttcagc
     3301 tagatatgtt gaataataac taattctatt gtattattaa tctgtagaat aaaactaaat
     3361 cggcaaaact agagaaattt agtgacgcaa agctataggg actaacgctt ataaaataag
     3421 ttatgtcagc cagttgccaa aaagatattt tctttttgtt tttcatgaaa gttttttagg
     3481 gggataaata cattatgttt agagataaaa tggataaatg tacacatatg ttaactgctt
     3541 atattggtag ttcatatgat tattgtgatt ttatagatac acagttagat gattttatat
     3601 tagagtacgg agaaaaagtt gtggaatctt gcttgcatca agtgatggta ttggtaagta
     3661 agtataatta gacagatttt tttctagaaa acccttttta gttttatcta catttacatt
     3721 aatatattgg taatataaaa tgtttaaatt tattttcata ttaagaataa ataaaaatgt
     3781 taactaaaaa ttataattaa atagacattt atgttaatct atgatacaat agtatttgtg
     3841 gtaatacaca agtaaatcaa gtatatcttg atataggtta ataggagtat ttttaatgaa
     3901 taatggaata gtaaaatggt ttaataatga aaaaggattt ggatttatat caatggaaag
     3961 tggagatgac gtatttgctc atttctcagc tatacaaact tcaggattta aatcattaga
     4021 agaaggtcaa aaagtaagct ttgatatagt taaaggcgct agaggtcctc aagcagaaaa
     4081 tataactata ttataatata tagatttttc ggaaagactc ttttaagagt ctttttttta
     4141 tttacgcatt ttgtaaaaaa ggaaaaaata acttcaacaa gaaaatactt aaaaataata
     4201 cttagtctaa atacttttta taaaataaat ctgacaaaaa tatatttata aagtatgtac
     4261 ttaaacctac aagtactctt ttaattagaa tatatagtaa tataataatt ttagttgtgg
     4321 cacatcaaaa ttttcaacta tctcataaat acatactctt tcttttgtat tcaattctga
     4381 tataatactt ttagtaattt gttaataaaa agcaattaaa acatatctat a
//
LOCUS       DAEORZ010000221         4429 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEORZ010000221 DAEORZ010000000
VERSION     DAEORZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.227"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(140..343)
                     /locus_tag="KRH39_003631"
     CDS             complement(140..343)
                     /locus_tag="KRH39_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG8513498.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            635..1399
                     /locus_tag="KRH39_003632"
     CDS             635..1399
                     /locus_tag="KRH39_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG8513499.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            1788..3101
                     /gene="prdC"
                     /locus_tag="KRH39_003633"
     CDS             1788..3101
                     /gene="prdC"
                     /locus_tag="KRH39_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBG8513500.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      3504..3589
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            4170..>4429
                     /locus_tag="KRH39_003634"
     CDS             4170..>4429
                     /locus_tag="KRH39_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HBG8513501.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQIPDGEKPSDSQVPDGEKPSDGQVPDG"
BASE COUNT         1786 a          484 c          782 g         1377 t
ORIGIN      
        1 tgtacaaaat taaaagaaca atttgtattg tattattaat ctttaataat tcatataaaa
       61 acaattttat aaataaatat agaaaaacag ggctgctaat tagcagccct gttgataccc
      121 aggtgtcaaa atttactatt tattttccag ccatttgtct ttcagccatt tcaactaatt
      181 tcttagtcat atatccacca acatatccat tttctctagc tgttaagtta cctttgtcta
      241 tgttttcata gttagataaa cctatttcgt tagctatttc taatttcatt tggtttaaag
      301 cagcttttgc ttctggaact actgttctgt ttgaatttga catagtaaat ttcctcctca
      361 aataattagt ttatgatgtc taacaacact tttatttatg tgatgttaat actaatttct
      421 ccaaaataaa aataatatat acagaaaaat aaaggtaaaa atgtaaaaaa tataaaaaaa
      481 ttaaataaat gagtatttta gagagtttaa ttaggtcaat ataggtttta tttttttagt
      541 tattgtctta taaattaaat attacagtac ttttttgagc tgaaaaaaaa taaattttaa
      601 atatagaaga aattatttca aaggggggaa tagtatgaaa agagaagtta aaaggactaa
      661 agctttgata tatagtgttt tatttttttc attattatta gtgttaatat ttgtttttgt
      721 ttatggaata aaatacatat gttctaaatc tgacaattca aaaatgccta tatataaagt
      781 tgatacaaat gagaaaaaaa tagctttgag ttttgatgta gcatggggaa ctgataatat
      841 ggatgatatt ttaaaaattt tagataaaca taatgtaaaa gctaccttct ttttagttgg
      901 tagttgggtg gatgataatg aagaaattgt aaaagctata gatgaaaaag gacatgaaat
      961 tggaaatcat tcaaatactc atgccaattt gaaggaaata tcaaaagaag acataacaaa
     1021 agagatagaa actacatcag aaaaaatata taatataaca ggaaaaagga ctaatttatt
     1081 tagacctcct tttggggacg taaacaataa agctatggac atttgtagtg atttaggtta
     1141 taaagtaatt aagtgggatg tagattctat agactggaag gagcttggac caaatcatgt
     1201 aattgaaaaa gtaataaaag aatctcaacc tggttcaata gttttattcc atgctaatat
     1261 aaatgatgta gataactatt tggatactat aataagtcga ctaaaaaaag atggatacag
     1321 tctagtcaaa atatctgatt tgttgtataa agataattat gtagttgatt ccaatggagt
     1381 gcaaaaaact aagaattaat aagtaataaa tgtatatttt aagtaaagtt gtgataaatt
     1441 atatagtata tgttagaaaa gtgtcgataa aaagacggtg tctatttttt gacacttttt
     1501 atacatgtat gcaatatcaa taattataga ttaaattgat taaaaaagtg tctaataatt
     1561 gacttttttt tgatgggttt aaaaatttag aagaattaat gaatgctgta agacagtatt
     1621 tccaaagggt ataagtttgt ttaaaagttg gcatagaaat tgctttgtta ataagcgata
     1681 tgttctatag aaataaacaa tatgttaaga ccttataata gaaaaatagc attaacatta
     1741 tttgaacagc ttataaaaaa attaatatac ttaagggggc gtactagttg aacaaactat
     1801 attctatttt attaaaacaa cacgttggtg gtccagataa gccagtagta agtgttggag
     1861 atgtagtaaa aaaaggaact ctaatcgcag aaccaactgg attaggggca aatatttatg
     1921 ccagtgttag tggaaaaata agtgaaataa atgaccaagc gatagtaatt gaagctgatg
     1981 aagtacaaga agatactttt gaaccattaa aaggagaagg tatacttgac ttaattaaag
     2041 aagctggagt agtaggaatg ggtggagcag gattccctac tcatgtaaaa ttaaacatag
     2101 atttaaatgg tggaacaata ttagcaaatg cagctgaatg tgaaccttta ttagctcata
     2161 atataaaaga aatagaagaa agaccagaaa tagtttatca aggtataaaa tatgctatgg
     2221 aagttactaa tgctggaaaa ggtatgctag caataaaaag taagcatcca aaagcaatag
     2281 aagctttcaa aaaagtaata aagccaggag acaacataga agttgcagaa ctagttgata
     2341 tgtacccaat gggagaagag agagcaatag taagagacgt tctaggaaaa ttacttgagc
     2401 caactcaatt accttcagag gctaatgcag tagtaataaa cgtagaaaca ctaactagaa
     2461 tagtagaggc tgttgagcaa aaaagaccag ttatatctaa aaatataact gtagttggtc
     2521 aattaaacag tggaaaagaa tctatagtgt ttgaagatgt gccaattgga acaactgtcg
     2581 gagaattaat tgaaagagct ggtggaataa aaggtgaata tggtgaaata atcctaggag
     2641 gacctttcac tggtaaagct actactttag atgctccaat aactaaaact agtggtggta
     2701 taatagttac tatgccattt gttaatgaaa agagaaaaat gggtctttta gtttgtgcat
     2761 gtggacctaa tgaagaaaga atgagagata tagcaactaa aatgggagtt actgatatag
     2821 tatcagttca aaaatgtaaa caagctcaag aaattaaagg tgcattaaaa tgtgaaaatc
     2881 caggacactg tccaggacaa gctcaaaaat gtattgaatt taagaaagct ggagcagaag
     2941 ttatattaat aggaaactgt actgactgta gtaacacagt aatgggttct gcaccaaaat
     3001 taaaattagg tgtatatcat attactgacc atgtaatgag aacagttaat cacccattaa
     3061 taagaagaat aaaaggtgac gtaagattaa aaataaaata atagataaat agaagttaga
     3121 catctttaga agtaaaactt aaaagttact tctaaagatg ttttttaatg tattttgagg
     3181 gcaatatata ttgtaaaata aatggaaaaa atagacaaaa tatgctagat attgtataat
     3241 gataacaata gctattacaa ttaatattgg acaaaaataa tgatactcaa gtatgtggtt
     3301 tgtattaata tgcatattat attaagtcaa ctatccgata gagtaaaagg tttttgtgat
     3361 atttgaagat actctatagt atctttttta ttataaaaac taatttttgt cttttattag
     3421 ataagtatct aggatactaa tttatatttt attgagttgt taaaaaagtg cacttaaaaa
     3481 gttaaaaaag aaaaattaaa aaacctattt atagaaactg tgaagtatat cttaaacctg
     3541 ggcacttaaa agatatatgg agttagtagt gcaacctgct ataaatataa ataggagtaa
     3601 cttttaattg tcaaaaatat ataaaataaa tacataaata agtaattaat agaagataga
     3661 tataaatata tttttgaaat gctccctgca ttcataacga gtgtagagtg gcagatatct
     3721 gcccaaaact cctttaattg ctggaagttc ctaaagctaa ctaaaccaca acataatcat
     3781 gaaataagga taagtgtgat agagtgtgca tttaataagc acttgaggtt acgaaagtag
     3841 aaaaaattag ttagatagca taaggttaaa tcctaagtgc cttaaaatgg ataatcagca
     3901 ggtaagcttc gaaaagaaga aacttcaacg actagagaga aatctcgtac ctacaagtgt
     3961 ggggaaatgg ggagtaccta atttatggtt aggtaatgat atagtctgtg cttaatagaa
     4021 atattaagaa gttcatagga gaactggtta ggagtaacga ccctaattga acacactaaa
     4081 gatgtattat tgaataagat tcatataata tttaataatt ttaattatat taagtgaata
     4141 caatatatgt ttttagtatc aggagaattt tgaaaacaaa aattaaaaaa tcaagtataa
     4201 tcagtttagt aattgctttt agtatggtat ttactgcatt tacaccaata gtatcttatg
     4261 ctgacgaagt gactagcaat gatacaatac ttaatggaga agaacaatca aatggtcaga
     4321 caccagatgt agagaaacca tcagacggtc agataccaga tggggagaaa ccatcagaca
     4381 gtcaagtacc agatggggag aaaccatcag acggtcaggt accagatgg
//
LOCUS       DAEORZ010000222         4313 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEORZ010000222 DAEORZ010000000
VERSION     DAEORZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.228"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            507..1373
                     /locus_tag="KRH39_003635"
     CDS             507..1373
                     /locus_tag="KRH39_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG8513502.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCSNLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIIDLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKTNLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            1486..1890
                     /locus_tag="KRH39_003636"
     CDS             1486..1890
                     /locus_tag="KRH39_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBG8513503.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            2066..2515
                     /locus_tag="KRH39_003637"
     CDS             2066..2515
                     /locus_tag="KRH39_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8513504.1"
                     /translation="MKSEIILEMKQINSTEYDEMLQVWESSVRATHDFLTKKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKAFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2672..3049)
                     /locus_tag="KRH39_003638"
     CDS             complement(2672..3049)
                     /locus_tag="KRH39_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG8513505.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPVMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            3311..4228
                     /locus_tag="KRH39_003639"
     CDS             3311..4228
                     /locus_tag="KRH39_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBG8513506.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDSPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVAKQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWNTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGDEMVGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKIQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
BASE COUNT         1699 a          472 c          694 g         1448 t
ORIGIN      
        1 aagtaataaa agtttgttta tttatcttta tattttctta tggttttatt aaatatagat
       61 tttattgaac accaaaatat tctctccttt acaaataagc caatcttatt taaactaata
      121 tactttatac tatttatact gaaattgtaa atttaaatgc tatgttattt aggtttatat
      181 agttaattta tatatttctg attttataaa ttgtatttat tattatatct taattctaaa
      241 atcattaaaa atttcaatct ctataaagat ttacaatttc tcaaataaat tattagtata
      301 taaacaattt attattacag tgttgcattt aaaataagtc ttaatataaa ggtattgtta
      361 caagtataaa gaaacttatg tatgctattg gaataaataa taccttaaat tataattaag
      421 aataagaact gttaaataag ggacttgatt atcatttagt tatagataat ataatattaa
      481 aaataaagct taagaggaga aaagatatga tagatataca taaaaatata tatgataata
      541 agttatttga ggagctaaag attgactgta aaaagtgttt tggactttgc tgtgtagctc
      601 tttatttttc tgcttcagat ggatttccta ttgataagga atcaggaaaa ccctgtagta
      661 atttacaacc agattttaaa tgttctgtac ataatagtct tatgaagcga ggttttaaag
      721 ggtgtactgc atatgattgt tttggttcag gtcaaaaagt agcacaagta acatataagg
      781 ggatagactg gatgcaatca ccagaattaa caaatcaaat gtctgaagtg tttttaatta
      841 tgagacaatt acatgaaatg ttatggtatc ttaaagaagc atctgtatta aatatatcag
      901 atacaatcaa atcaaaaatt gatttaataa ttgaagaaac tgaaaagata actaatatga
      961 gtccagaaca gataattgat ttggatatca tatcacatag aacaaaggtt aatttattat
     1021 tatcacaagc gagtgagtct gtgatgggaa aggttaaatc atttatcaag acttctactc
     1081 ttaaaaacat gaaaaaatta agtaaaaata tagatttaat aggagcagac cttagaaaaa
     1141 caaatctaat aggagcagat ttaagaggta gatttctaat agcagcaaat ttaagaaata
     1201 cagatttgag tggggcaaat ttaataggag cagacttaag agattgtgat attagaggtg
     1261 ctaatcttga aaatagtata tttttaacac aattacaagt aaatactgca aaaggagatt
     1321 ctagtacgaa gttgccagct tcactaatac gccctaaata ctgggagaaa taattaaatt
     1381 aatatataat tttacaagat atgaactttt ttgactttaa ttcatattat acattagaga
     1441 ttctttacaa gaatttctta atataatata gggagagata taaaaatgag ttatcaaaat
     1501 tataaatgtg aaagagatta tgtgtatgat gattataaat acaataagtg caacaagtgc
     1561 tataatgatt atgaggaaat gacacatgtt catgaatatt cagaaagtgt taagttagca
     1621 gaagaatgtg aggatagaca taaccaccgt gcagcaggag ttactggtga agttatacca
     1681 ataaatggag gtacaaatca tgttcataaa ataaatgata atgtagattt ccttgaccat
     1741 ttccataaga tatgtgttac aacagggcca gcaattagaa tacctggaac tgataaacac
     1801 attcacttaa tatgtggaga aactactgtt aatgatggtc attgtcataa attccttttt
     1861 actactcaaa tagaagctcc tttagtataa gctgatgtga attattgtag taaatttgct
     1921 agattatagt atatagatgt taaaaatata taatagataa tatttaaact taatgaggac
     1981 tatgatattt aaccttcgta gtcctttcta agttttgtat gaagaagtgt tatacaattg
     2041 aaagtgatat agggagaaaa tttttatgaa atctgaaata attcttgaaa tgaaacaaat
     2101 aaattcaaca gaatatgacg aaatgttgca agtctgggaa tcgtcagtaa gagcaactca
     2161 tgatttttta acaaaaaaag atattgaatc tttgatacca ttagttgaaa ttggacttaa
     2221 agaagtagaa aatatagttt gtataaaaga taatgatata ataaagggct ttataggaat
     2281 agataaagat aaaattgaga tgctctttat agaagataaa tatagaggaa atggtattgg
     2341 taaaaaatta ataaaatatg ctatagataa atacaatgta aaatatgttg atgtaaatga
     2401 gcagaataaa aaagctgtag gattttatat tcacttaggg tttaaagcgt tcgataggtc
     2461 tgaaatagac ggacaaggta acccatttcc aatactacat atgaaattaa tttaaagtta
     2521 tacttctcac ttattcgcaa aaatatttac ttaaattttt tatacaaata aaagtcgtga
     2581 cagtcttagg acacgacttt cttactacac tattaaattg aaatttatct gttctatatt
     2641 atgattatta gatgtagttt ttttatttta actatgtact taaataccaa gtatacccat
     2701 acctatcaag tacttcagca gcgccatcat tttgatgtgg cggtgggtgt ggtaaaaatt
     2761 cttgtacaag cgtaccaccc tcggcaagtt ttacagatat ttcttctatt gcttctcgag
     2821 atggtagaac aacacaaatc gccatgccag aaattacaac aggcttgtct gtatccgcac
     2881 ccattataca accttcatat tttgtgaaag taacacttgc gtgcataaca ggattgtttg
     2941 gattgctgtc taatcgcgca aatatagttt ttcctcctcc gaaagcctca gcataaaaat
     3001 taaatgcttt ttcacaagta ccattaaata ttaaatatgg agttagcatg acaaatatat
     3061 cctccttgat atagaataat ttatagttgc acctgatagt ctaaattctg atttagtaaa
     3121 ttgtatcata ggtaaacctt gatgtatata attttttatt attagattat acaaatttat
     3181 taattaaaaa aaatgttata tgtatggaag atttacagga taaaatataa taaaaagatt
     3241 aagatgacat tataaaaaat gtatattgac attgacgcta cgtaattgta tatactttgt
     3301 tttaaaggag atggtaaata tgaatattat agaaattcgc tcagataaaa tatacaagaa
     3361 gataatggat tcaccaataa acaaaaaaga agatatatac agatatgaat tgatgaagcc
     3421 ttttgaattt aagtggaagt gtatgaatgt tccaatagtt gctaaacaga aaggcggata
     3481 tgatgtaatt atagcaagtg aaatgttagg ggttttatcg cctaaggata ttgatgaaaa
     3541 gcaaaaaaag aatataaatg tgttatctgc tgataaaatt tggaacactt gcaaagaaac
     3601 tatagaaaac tctataaatg cttttataaa agaagggtat gatttaaaca ttaaggatta
     3661 taaatattca atattattgg cgaatccaaa tagcccttac acaatattaa gtgatggata
     3721 ctggggtgat ggtgggattc ctggatatat atttctatca ttggttccta atgaatatac
     3781 tatcaataga ttaccagtat taatagcaca tgaatgtaat cacaatatta gattccagtt
     3841 tatagagtgg aataataata taacattaga agaaatgatg ataaatgaag gtcttgcaga
     3901 aaattttgca acatggatgt ttggagatga aatggtagga ccttgggtca gtagaacaga
     3961 tatcgaaaca ctaaatactt atataaagcc aataataaaa agtgctttaa aagaaactgg
     4021 atttcaaaat ataacatctt atctttatgg tgatgatata gctaaaatac aaggatattt
     4081 tccagtaggg ttgccttatt gtgcaggata tgcttgtgga tattatatga ttaagtatta
     4141 tttagaaaag acaaataaat caataatcga agccacttta ttgccttata gtgagataat
     4201 agaagcagta aaagagtttt gggaataaat tttataaatt gcataatttg aaataaatta
     4261 tgcaattatt ttatttttaa gaaaaaataa attgacaata atttataaga aat
//
LOCUS       DAEORZ010000223         4303 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEORZ010000223 DAEORZ010000000
VERSION     DAEORZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.229"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(296..1567)
                     /locus_tag="KRH39_003640"
     CDS             complement(296..1567)
                     /locus_tag="KRH39_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG8513507.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKESSMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTLDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     repeat_region   2214..3232
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2214..2242
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
BASE COUNT         1602 a          487 c          551 g         1663 t
ORIGIN      
        1 catgttagat atatcattgt aaattaacat agctttgaat ataataatgt tcagaaggca
       61 atatttttga ggtctgaatc caataataat atagtcttat gattgtagat atcaaaaaat
      121 gtaagttact aggaatacgt tttgtagatt tatatatgat taataactaa agctataaat
      181 aaacaaaata aaaagagtat gtttcaacct atagctttta gatttaaaat atactctttt
      241 attaattata gttgtataaa ttaaccataa tatataatta aaatttttat tataattata
      301 tattaggttc ttcaattagt tttccacata catatctatg aatgatattt ctatcatcac
      361 caacatagat aaatctttca agtctttcaa gtatagaata attgtcatga tttaaatttg
      421 aatcatcaat aatcaatgca tcaaaatcat aaccttcttc aaagctacca acttttccaa
      481 agaaacttcc accacttttg gttgcaatat aaaaagcctc agataaagat aagaaattat
      541 acttcttacc agaattaacc caatataatt tagataattg tattgcagat accattgctt
      601 taaatattga caatgtatgt ccaccgctta tatctgatcc aagtgctaat ctaatattat
      661 tatcgattaa ttttctgata ggcatagcac cactaccaac attaaagtta gaagctggac
      721 agtgaactgc cataacatta ttttctctca ttaaatctat ttcatcctga gaacaatgaa
      781 cacaatgagc cattaaagta ggagtttgac caaataaatt atatctatta tatgcatctc
      841 catagaattt agattcaggt tctagacttc taacccattc aatctcatca taattttcag
      901 ataagtgtga ttgaatagga atattatatt ttaaacataa atctcctaaa ccttttaaaa
      961 gttcacttgt acagcttggt ataaatctag gtgttattat tggttttaca atagaatctg
     1021 tatcactata cttatttata atttcttctg tatcagtaat agaatccaga gtgttttcta
     1081 ataaagtatc agggcaattc atattcatat ttactttacc aacgtatgct ccaagaccaa
     1141 cttctttgaa tatatccata agtttcatag aggattcttt atgtactgtt gcaaacactg
     1201 ctattcttgt agttccagat ttccaaagtt cttttacaaa ttttttatac atttttgttg
     1261 catattctat atctttataa tttgattctt ctggaaatgt ataagtttca agccatggca
     1321 aaagctcttt atccatagcc attccaagat ttttaaattg agaggcatgg gcatggaggt
     1381 cattcatacc aggtattata agcttatcag tgtaatctac tacttttagg tttttgtatt
     1441 ccttaggtat ctctttaaat attcccttta ctttaccttt ttccagtata atataactat
     1501 tttcaaatac tgtgaaagtt tctgaagttt ttgtaaaaat tatatttcct tttaatactt
     1561 ttaacatata ggtctcctta gttttaaatt tgttttaata tatgtaggta aaattttgtt
     1621 ttacaaatca aaattttacc acatgtatta tatcaaatct aaactataaa tactattaga
     1681 taatagcaaa tattagtgaa atacagttgt ttactattat tatatataac tgacatttaa
     1741 gtgacattta agaaaaatat aatgcctact tacataaaat ggaatgttat ttaaagagaa
     1801 ctttgattat attttcagaa gcttttttat ccatatcgtt taaaatatga gaatatctat
     1861 ttatagttat ttttatatta gtatgtccta atctttcaga gatgattttt atattagttc
     1921 tagctagaag aagaactatt agaatagata atatagtaag tatttacaaa tatgtaggtg
     1981 ttcttaaatt gataaattat tccattttaa ttttatagtt tgaattttat gatataataa
     2041 aaatatataa attttgcagt gagcgatatt tttgataaag tagggtttaa cagttgcaat
     2101 gtaagggatt gagggtgtat gataaatgtt atcaattgca ctactcatgg ttcactgcaa
     2161 atttgagaga gttgtatgtg tgtaagtact gaaaatactt agtttatttt ggggttttat
     2221 attaactaag tggtatgtaa attactgaaa cccagccata tacatattta catactccag
     2281 ttttatatta actaagtggt atgtaaattc cattcttgca tcacttgctc cagtagtcat
     2341 aacaacagtt ttatattaac taagtggtat gtaaatcatt attgttgctg aataaatcca
     2401 cttctcattc tcaagtttta tattaactaa gtggtatgta aatatcgtaa gaaccaggaa
     2461 tatacggaag atcgtagaaa agttttatat taactaagtg gtatgtaaat ttttttgttt
     2521 ttgcttcatc ttttttatct ttattcagtt ttatattaac taagtggtat gtaaataaaa
     2581 ttgctgaaac aatagtgata agagaagatg ctggttttat attaactaag tggtatgtaa
     2641 atagagttga ggttgagttg gaggatatta tttaagggtt ttatattaac taagtggtat
     2701 gtaaattttt tattctaaga aacttttcaa ttctagttct tcgttttata ttaactaagt
     2761 ggtatgtaaa tgctttttag cttcgtctac tactttataa gcaaagcgtt ttatattaac
     2821 taagtggtat gtaaatcaac atgtcagtaa tattgaaaca ggaaattcga gtgttttata
     2881 ttaactaagt ggtatgtaaa taacacttta aaaactaatg tattcagtat aagagattgt
     2941 tttatattaa ctatgtggta tgtaaatctt gttaatgcaa ataaacaaca tagcactatt
     3001 aatagtttta tattaactat gtggtatgta aatagggttt ctgataaaat cttcaaacat
     3061 gtaaaatatg tgttttatat taactatgag gtatgtaaat cttatactta gttagaacta
     3121 tatatcgaca caaatatgct ttatattaac tatatggtat ataaattgca atttttattc
     3181 gttgtccaat cgctttgaaa tttgttttat gttaactatg tggtatgtaa atttgcaaca
     3241 agtataggta aaatacccca ataatttata cagcattttc tcctttaaaa tataattatt
     3301 ttttagcatt tgtagtaaat aattaccaga taacattgac tttagtttta atgattaaaa
     3361 tataaaagta gaataattat aaaaagtatt gaaaaattta taaatatata taataaaacc
     3421 taatgacaag atattagata taaaaaataa ttaccttata aatagattga aatttatgaa
     3481 tattcatact ataatttaaa tataaggaga tgccctttga aaataaaaat taaaaaatat
     3541 ttaatgctat tacaatagga actagaacta cacttaataa atatacagaa attagatttg
     3601 gctcaatata aaatacaaat atagaattta ggtgtttttt atgaaaaaat ttttatatgc
     3661 tttatatagt ttcattgtta ttatagctaa ttttagatta aaagaaaaaa attataactt
     3721 tattttatta gcaatctttt ttatattgct gttaattcaa aatggagttt taaaaacagg
     3781 ttatcaaaat agagctacat tttatcaaga taattatttt aatagagagg ataaaaatga
     3841 taagataaaa tttatgacta taaaaatttt tatatttttg tcattaccat tatgttttaa
     3901 tatactattc aattatttaa cctcttaaat tatatttaga tataggagtc ttatgataaa
     3961 agtaagctta tgtgttattt gtagtaaata taaagtgcat atagtgataa aacaaaagtt
     4021 tcatataaat gaagaaaaaa atataaaata atttatttaa ctacttaaaa ataaactaaa
     4081 agggataata aaatagcttt taggttatgg taataaaggt atttaaaaag agtgcataag
     4141 cactccaatt gtgagaaata catacacaaa tcattattac aacaatgaaa aatgtacata
     4201 taagagacgt tttgtatttg ttttaagtga taatatttta atactaacta aataattatc
     4261 acaatagtaa aaaagaaaaa ggagtatttt gtactccatt tgt
//
LOCUS       DAEORZ010000224         4292 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEORZ010000224 DAEORZ010000000
VERSION     DAEORZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.230"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            231..1310
                     /locus_tag="KRH39_003641"
     CDS             231..1310
                     /locus_tag="KRH39_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG8513508.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLAIFGYDDTTSINLQDFTLETTSISIGEDLTFSFK
                     ILAKKATKTRLEYGIDYVKSNGKKSRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="KRH39_003642"
     CDS             1634..2002
                     /locus_tag="KRH39_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG8513509.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2916
                     /locus_tag="KRH39_003643"
     CDS             2119..2916
                     /locus_tag="KRH39_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8513510.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDRQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQL"
     gene            3310..4200
                     /locus_tag="KRH39_003644"
     CDS             3310..4200
                     /locus_tag="KRH39_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8513511.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDSCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
BASE COUNT         1637 a          476 c          812 g         1367 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaatttat atggtttata gtttaacagt aggaaatatc
      121 gtatgagtga gaatatcgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagtatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg gtcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagaacgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggtt gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacctg tgctacctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtacgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgc agaactctgc taaaaaaagg caatcgtgat gtattagcca
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaagat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattatg taaaatctaa tggaaaaaag agtaggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tgcaataccc atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaagatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca caatggagat tagataaaca
     1981 tattggaaag ccaataaagt agcgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccaa atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gatagacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaattgttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgatgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttataatata taaaaattac atacccatta
     2941 ggtaaattat gtagtatatg tgtttacaga tggttataga aataaatatg taataaacta
     3001 cttaacactt aaaaagtgga tttgtccaga gcataataca aagcatgata tatataaatt
     3061 tttaagcgaa atacatcaaa agaaggatta gaattagtgg agataaaaca gctattagag
     3121 attataaata aatagtttta aaggtactac gaatatagag gaaggtaaat catacgttat
     3181 tcaaggatta taaatgatta gtttaaaagt actatcggat aggtctctgc atatttaaac
     3241 cttagtaaag gtagttaaag tagtagttta aaaactaaaa aattatataa aggaattagg
     3301 tataagaaaa tgattgagag tataccagca aaacaaattt tacaaaaagt gaaatttgac
     3361 aatacaagat ggtttggaat tgactataat atgaatctct atcgtggttg ttctcatgga
     3421 tgtatatatt gtgatagccg tagcacaata tacaacatag agaattttga caaagttaga
     3481 tataaagaaa atgtgataga aattttaagt aaagaattgc gttcaaaaag aaaaaaaggt
     3541 gtagtaggca ttggagctat gtctgacaca tataatcctt ttgaaaagca actttgtatt
     3601 acaaagcagg cactagattt gatttcggaa aatcattttg gagtgtctat tgatactaaa
     3661 agtagtttgg tggttagaga tataccgatt ttgcagaaaa ttaaaaaaaa taattctgct
     3721 attgtaaagt tgactataac cactgcaaat gatgaattat caaagaaaat agaaccttat
     3781 gtaaatccaa gttctgtacg atttgaggca gtgaaagaac ttaatgattc tggaattttt
     3841 tgtggtatat tattgactcc tatgcttcca tttttgactg ataataaaga tgaaatacga
     3901 gcaattgtag aaaaagcaca caaggctaat gcaaaattta tttattgcat gtatggtgta
     3961 acaatgcgta gtggtcagag agagtttttt tatgagcatt taagagatat atcaccaaaa
     4021 ttggtactta aatatcagaa aacatatggt ttaaactatg tatgtactgt acaaaataaa
     4081 gattcttgtg agaagttgtt aagagaagaa tgtgcacgtt atggtatttt gacggatatg
     4141 aaagatatta taaaagcgta caagaggtcg gagtcatata ctcaaataaa atttttttaa
     4201 gataggcttt ggatgagata taatagtggt gattgatgtg ttgatattat agttgattgt
     4261 tagagaggat attatagatg aatattagta tt
//
LOCUS       DAEORZ010000225         4262 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEORZ010000225 DAEORZ010000000
VERSION     DAEORZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.231"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            138..1058
                     /locus_tag="KRH39_003645"
     CDS             138..1058
                     /locus_tag="KRH39_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBG8513512.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            1173..2330
                     /locus_tag="KRH39_003646"
     CDS             1173..2330
                     /locus_tag="KRH39_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG8513513.1"
                     /translation="MNNLDDIPVMPTLTLDPFGKESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMTPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(2415..3914)
                     /locus_tag="KRH39_003647"
     CDS             complement(2415..3914)
                     /locus_tag="KRH39_003647"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBG8513514.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         1688 a          579 c          635 g         1360 t
ORIGIN      
        1 atgcttatat ttaagtctat tactttattt gaaacaattt gtagaatttc aaattccttg
       61 acagtaataa gtaagtaatt gtataatcaa actgcaatat attaataata ttagtgaaaa
      121 tgaaaggatt ataaaatatg tataaatcag aaaatttttc aaaaaaatct gtattaataa
      181 attcattttt aagtaaattt ttaaaaataa ttggatgggg tttggtaagc ttatttacat
      241 tatcagcctt agtactttca actatttcca ttataattgg atgggctgta atacttccta
      301 ttcaagtatg tgcagtattt ttacttatag gaggagtttg tatttttaaa ggtaagagat
      361 taggagacca aatttctcgt tataaaaaat attgtacaat tataaacaat agaaatatta
      421 ttccagtaga acttattgca gaaaacacat ctaaatcatt aaattttata ataaaagatg
      481 tacaaaagat gatagacaaa aactacttta taaatactta tattgataaa agaaataatc
      541 agattgtgtt aacaaatgaa gattttattc caccaagata tgaagatgta gcatatgaag
      601 tcaaagaaga agaaaagact gatgaatcca atgaagttga tacaattata aataaaggaa
      661 tgaattatct aacacaaata aaagaagcta ataaaaatat taaatcagaa tcaatgggcg
      721 ataaaattgt acaagtagaa gatgttacaa gtaaaatatt tgatgttgta aaacatgacc
      781 catcaaaact tactcaaata caaaaattta tggattatta tcttccaacc actttaaaac
      841 tattaaattc atatcataca ttggaagaac aaggaattga tagagaaaat ataacaacaa
      901 ctatggaaag tattgaaaat acaatgaata cgattgtagt agcttttgaa aatcaattgg
      961 actatttgtt tgaagatgaa gccattgata tttctacaga tattactgtt ttagagaata
     1021 tgttagtcca agaaggatta acgagtggta aattttaact tcaatagaaa aaatattctg
     1081 ttatttttaa acaattaatt agtcataaag tataaaatgt gtattatata agttaatcag
     1141 tactttatga aggaagagga gagaaagata caatgaataa tttagatgat ataccagtta
     1201 tgccaacttt gacacttgac ccatttggaa aagaatcaaa cactaatgat atagataatt
     1261 ctgacttatt aatgaaaaag gatgaaaaag acccagaaga agaaaaacta tctgaatctg
     1321 aacgcaagat ggtaaaggaa tttgcggaaa aaatagatat tacaaataca aatatgattt
     1381 tgcaatatgg agctggtgct caaaaaaagg tagcaagttt ttctgaaaca gctttaaaga
     1441 gtgtaaaaac aagagattta ggagaagttg gagatatgct tacaaatcta gttactgatt
     1501 taaaagcatt ttcagcagat gaaaaagaac aaagtggttt tttaggaatt tttaaaaagg
     1561 caaataataa aatttcaaat ttaaaaacta agtatgatag tgcagaggta aatgtggata
     1621 aagtaagcaa agagcttcaa aagcatcagg ttaaaatatt gaaagatata gcaatgcttg
     1681 ataagatgta tgaattgaac ttagcttact caaaagagct tactatgtat attatagcag
     1741 gaaaacaaaa attaaaggat atgaaagaat atgagatgcc aaagttgaga gaaaaagctc
     1801 gtttatctgg ttcaacagaa gatgctcaat ctgtaaatga tatggtttca ctttgtgaca
     1861 gatttgaaaa gaagattcat gacttagaat taactagaat ggtatcgctt caaatgactc
     1921 ctcagataag attggttcaa aatagtaata atctgatggc agaaaagatt caatcaacta
     1981 tagtaaatac cattccatta tggaaaaatc aaatagtgct tgctcttgga atttctcact
     2041 taaatcaagc tatgaaagca cagcgtgaag ttagtaatat gacaaatgag cttttattaa
     2101 agaatgcaga aactttgaag atgggaacaa tagaaacagc aaaagaatca gaaagaggaa
     2161 ttgtagatat agacacaatt aagaaaacga atcaatctct aatatctact attgatgagg
     2221 tagttaaaat acaacatgat ggtagaataa agagacaaga agcagaagtt gaattatcaa
     2281 aaatagaaaa tgaacttaaa agtaagcttc ttgaatttac agttaaataa aaattatact
     2341 aatataaaaa gagactgtct caaaatagag ataagtctct ttttcattgt ttaaatgatt
     2401 aaagtatatt tatattataa atttctatta tcttttaaca taacatcatg agctccacct
     2461 tcaactatac tagtagaaga tacaagtgta agtttagcat ttttttgaag ttcaggaatg
     2521 ctaagtgctc cacagttaca catagtagaa cgaactttac ttaaagaaag agttacattg
     2581 tctttcaaag aaccagcata aggcacataa gagtcaacac cttcttcaaa agaaagtttc
     2641 ttatctccac caaggtcata tctttgccag tttctagcac gagcagaacc ttctccccaa
     2701 tactctttca tataactacc atttatatta actttgttag ttggactctc atcaaaacga
     2761 gagaagtatc taccaagcat tataaaatct gcacccatag ctaaagctaa agttatatga
     2821 tggtcataaa cgataccacc atcagagcaa attggaatat aaatacctgt tttttcaaag
     2881 tattcgtctc tagcttgagc aacttcaatt atagaagtag cttgtccacg tcctatacct
     2941 ttttgttcac gagttataca tatagaacca ccaccaatac caactttaac gaagtcagca
     3001 ccagcttcag ctaggtaaag gaatccttcg cggtcaacta catttccagc tcctacctta
     3061 actgtatcac catatttttc acgaatgaaa tcaagtgtta ttttttgcca ctcagaaaaa
     3121 ccttctgaag aatctataca taaaacatca gcaccagctt caactaaagc aggaacacgt
     3181 tcagcaaagt ctctagtatt aataccagct ccaactacat atctcttaga agaatctaaa
     3241 agttcaagtg gattttcttt atgagaagag taatctttac ggaataccat atacataaga
     3301 ttaccattat catcaagcac aggaagagaa ttaagtttgt ggtcccatat aatattgttt
     3361 gcttccttta aagtaacatc tttgttagca taaactatag atgacatagg agtcataaat
     3421 tcactaactt tagtatctaa atccatacga cttatacgat aatcacgact tgcaacaata
     3481 cccaataatt taccagttga agttccatct tcagtaacag ctactgtaga atgtccagtt
     3541 ttttctttta agtcaagtat gtcttttaat gtgttatcag gtttaatatt tgaatcactt
     3601 acaacaaaac cagctttgtg ggatttaaca cgtgctacca tagcagcttc attttcaata
     3661 gtttgagaac cgtatataaa tgaaatacca ccttctttag caagtgcaat agccattttg
     3721 tcatcagaaa cagattgcat aatagcagaa gtcaaaggta tattcatata aatatctgct
     3781 tcctcgccct ttttaaattt agtaacaggt gtttttaaac ttacattagc aggtatacaa
     3841 tcagtagaag agaaacctgg tacaagaagg tattcactga aagtatgtga tggtgtatca
     3901 aaataaaacg ccatttttta atcactcctt atatttttat actttttata attgatagta
     3961 ttaattataa cttattcttt aattttatta aagtattttg tcaaatattg agatttttga
     4021 aaaatttaaa ctattttaat taaatttgtt taatatatat tgccttatag ggtaaagttg
     4081 tatattaaat aatgtttagt gtttaatagt agctaattta aaattaatct ttagtgattc
     4141 atataattac atttgtatag tttatattga taatattact agagagtgca taaacctcaa
     4201 aaatacagat aaattaaatc tatattagta tatccaaaag tacatttaat aaatttattt
     4261 aa
//
LOCUS       DAEORZ010000226         4197 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEORZ010000226 DAEORZ010000000
VERSION     DAEORZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.232"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            385..864
                     /locus_tag="KRH39_003648"
     CDS             385..864
                     /locus_tag="KRH39_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8513515.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFENVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVIM"
     gene            861..1640
                     /locus_tag="KRH39_003649"
     CDS             861..1640
                     /locus_tag="KRH39_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513516.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKALDKEDNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEDYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1890..2390)
                     /locus_tag="KRH39_003650"
     CDS             complement(1890..2390)
                     /locus_tag="KRH39_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8513517.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELKLI"
     gene            complement(3002..3709)
                     /locus_tag="KRH39_003651"
     CDS             complement(3002..3709)
                     /locus_tag="KRH39_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG8513518.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT         1543 a          625 c          458 g         1571 t
ORIGIN      
        1 tagtcaagat actgttatct ttactcatta tttaaagaac ttattttaaa tctgtacatg
       61 aagtaaataa tccaaaagca ttgttattct aactactcaa cattaaatac ttactatttt
      121 ctaattactc aatataaaca cttactataa aactaaatac ataaatggct tctaaagttt
      181 aatatatcta catggcttta tatatgacag gaaaaatact agaaaaaata tattatagaa
      241 tacttgtata taataggcaa aactttcaaa cttaaattta tcaaataatc ataaaaaaag
      301 tgaaccatct atagtaaaat tttatctaat aagtgaaagg caaaaaagta gcgctacgat
      361 ttgcatagaa aggaaggaac ttagttggat aaaactacat ttacaaacaa tatacttgaa
      421 tcagaacaga ctttgtatcg agtatctaag tctattctag gaaacgacca agactgtgaa
      481 gatgccgtca acaatgctat tttaaaagca tatgaaaagt tggattctct taaggaagaa
      541 caatacttta aaacatggct gattagaata gtcataaatg aatgtaattc attaagacgc
      601 aaacgattaa aatctctatc atttgaaaat gtcttcaaaa acaaaaaaat tgatgaaaaa
      661 gatgactata gcgatttgta tactgctatt caaagtctac ctaaaaaaat taaaattcca
      721 atagtccttt actatattga aggttattca gtagatgaag ttaaggaaat attagatata
      781 cctcaaggaa ctgtaaaaag tagattatca agaggaagaa gattattaaa aactaaatta
      841 gaaaacacgg aggtaatcat gtgaataaaa atatattaaa taattcgttt ccagaagttc
      901 cagaaagttt tcataatagt ttaagcaaaa cactaaatag tttaccagaa agagaggaaa
      961 attataaaat gattaataat aaaacacaca aattaccatt tagaaaaggt ttagttgcaa
     1021 ctttagccat tactttgata ttgagcacaa cagcccttgc agtaggacat atatatagtt
     1081 tagttggaaa atcaagtact aaggctacct atacatctat cccaactaca gaacaagtaa
     1141 aaaaagactt taactttacc cctaatatag tttctgaatt tagtaatgga tataaattta
     1201 aaggagcata tactgtaaat aaaaaagcac ttgacaaaga agataatgtt ttaggaaaat
     1261 caaaagcact tagttttgaa tataaaaatg gcaatgatga aatatctctt gacacagcaa
     1321 ataatgtact tgggaagagt tctgacaatc taaaagttgt agatacttac aaagatatta
     1381 atatttctta tgaagcattc gcacaaaagt ttgtaccaga agattataaa atgacagaac
     1441 aggataaaat tgatgaaaaa tcaggcaaat acacatttag tgtagggcca aataatgaat
     1501 cagacaaagt tgaagttaat gaatttaagt ttttgacatg gaaacaagat ggagtttact
     1561 attcttttac agttcaagac tcaaatttat ctatgaatga cttagttaaa atggctcatg
     1621 aagtaataga tgcaaaataa acataagtaa taataagtcg tttatgagtt tattatttat
     1681 gactttaatt tttttattag taaacatggt tgtatcttcc atctttatat tttctttaaa
     1741 tttaatatat aaaattttag atatttagct accttcaaaa gaaccattat taaaaagccc
     1801 ttaaaaatat ttcttttata tggaatttta agggctttaa ctttatttta tacaagtgta
     1861 tatggaattt taaagacttt aactttattt tatataagtt taagctccat ttgaattact
     1921 tcttctttta caggtctatc attccatgta aaaccactag atattgtatt tacctctaca
     1981 aaccctaaag atttataaac atgaatagct ctactattac ttttaaatac atcaagctca
     2041 attttttcat acccatattc ttttgataaa tcaataattt gttttaaaag actacttccg
     2101 ataccttttt ccctatactc tttaagaata cccatgccta aataaccaac tttttcagtt
     2161 ttaggtaaaa cgtcacacca accaatacag ttatctgatt ctaaatcaat tataaacagt
     2221 tgtggtaagt ttttatttat agtatttttt ataaattcaa ctgtttcttc aaaaggaaag
     2281 gcttcatcca tagctagata tttcctctct tttgctattt tatcaaaagt ttgccaatac
     2341 gaataaatat acttttcttc tgcctttata aattttatgc cttctatcaa cttaacattt
     2401 ccccttttta atttttgatt gtagttaact tattttatat gattctagtt tataatgcta
     2461 tataaattac tatatattag cttaaaatat ctttgtatat ttctataaaa ttaaaaccaa
     2521 tatatctatc tcttgtatct ctattaaaat aatttttaat agtataataa atatagttct
     2581 ttaatctttt gggtatatat acttaatcat tttgaaattt gatttaaagt ataataaaat
     2641 atataaaaaa atatataaaa tggtactata aaatttttaa tcttcatcaa tacctttttt
     2701 gctttcattg ttaaaactat tttttctatt agtttatttc ttataaaaca aacatttagt
     2761 attaaaatat atatcacata atagtatttg caatttaact ttaaaaaaat aaaaataccc
     2821 aactgaccta agcaactcga ttgggtataa ataatctaaa aaatcatttt tgtataaact
     2881 ctttatttaa cttacatttt tatttacctt ctactttaat tcaacttata tttctatgct
     2941 tcttatactc tcattcaact tatatttcta cttatcttat acttttataa attcatcaca
     3001 attatttagt tctcgctatc aaagaaattg tattaaaata ctttccattc tctacttcca
     3061 tcattttttt gtcatttata gcatatgaat tatcacaatt tatccaatcc ttccaagctt
     3121 catcatgaca atttaaagct tcacatttct ctacaatcac agactccgat tcagaccaaa
     3181 gtttattcca ccatgtaatt gaatggaagt tcatattatc ttgccaaaat ggaatgagtt
     3241 catctggtat acaatccacg aaatcttctt ttaatccagg cattgccata gcaagtattc
     3301 ccccctcttt tacaagaggt gatatatgat tgtctaaaaa tccttctttg tttccaaaat
     3361 agtgatatga atcgatactt atcaccgcat caaagaaacc ttctgcataa ggtagttcat
     3421 gtgcctctgc ctgtattgga aatattttat catccaattt aaactcttta aatcttttat
     3481 agttttcagt tggttctatc cataaatctg ttgcaaatac agtagcatca aattcctttg
     3541 ctaaaaatat cgaagatata cccttaccac aacctaagtc taaaatacgc attccctttt
     3601 ctagttttat cttacttgtt aattcttcca atatttttat acaattaggt cccatcatat
     3661 ttttcatgag aaagtcctta tcgtatttat tactttttgt aaatatcatt ctatctttcc
     3721 tttccctatc agttattatt tattaaattt tatacgtttg attttttatt ttttatatgc
     3781 ttcttatatt gagcaatctc ttttttattc attctctaac agacaatttt aatgtgtaat
     3841 tttatccact ctgtgatagt aactttaatg aataatctta tctactgtga tagttaactt
     3901 tgatgggtaa ttttatctac tctacaataa tcaccttaat gagtatctta tctactctat
     3961 gataattatt ttagtgagta attttatcta ctccacagca atcgctttaa tgagtaatct
     4021 tatccactct atgatagtta ttttagtgag taattttatc tactccacag taatcgcttt
     4081 aatgagtaat cttatccact ctataatagt cactttaatg agtaatcata atttttatat
     4141 cattttagat acttactcta ataagaagtc attttctaat acttaataag ttttaat
//
LOCUS       DAEORZ010000227         4173 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEORZ010000227 DAEORZ010000000
VERSION     DAEORZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.233"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            81..833
                     /locus_tag="KRH39_003652"
     CDS             81..833
                     /locus_tag="KRH39_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBG8513519.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            820..1701
                     /locus_tag="KRH39_003653"
     CDS             820..1701
                     /locus_tag="KRH39_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG8513520.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGRLNTNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTILSGEIMY"
     gene            complement(1927..3282)
                     /locus_tag="KRH39_003654"
     CDS             complement(1927..3282)
                     /locus_tag="KRH39_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG8513521.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         1637 a          608 c          521 g         1407 t
ORIGIN      
        1 gacatttttt cccttagatt ataaaattaa attaataact acataggtag ttactaatct
       61 tttatcaaag gagtttctct atggataata taattaatga attacaaaat ataggattta
      121 ctaaatacga atctcaaata tacatatcat tattaaagga aagcccttta acaggatatg
      181 aaataagtaa attatcaggt gtaccacaat caaaagtcta tgaaaatata acaaagctat
      241 taaacaataa tataattatc aatgttggaa ctgaccctat taagtacata ccaataaacc
      301 ctaatgaatt attaaaaaat aagaaaaaag aatttgacaa ttctatggat aatctaaaga
      361 atagcttaca gatattaaat aaaagtaaaa gtgtagaata cgtattaaac ataaaaggag
      421 ataaaaatat attaaaaaaa gcaatagaaa tgataaaaaa tgcaaaacaa gaaattataa
      481 tttcttgccc ttatgaagaa attcttgaat taaaaaaaga actcctaatt gcttacaaaa
      541 aaaatattaa tattgaaata ctacttttag aaaacaaaag tctgaaaggt cttgataata
      601 ttcatattca tggaggtaat aaaactacac ttgaaaataa aatgctttac aaaggaatta
      661 ttttgattgt agataatgat gaaatattga caggaaactt atcagaaggc agtgaggcca
      721 tttcaatttg gacaaaaaac tctaacattt tatatattgg agttcaatac ataaaacatg
      781 aaatttatat ttcagaacaa ataaaaggag gttataaaaa tgaacaaaat taagattaaa
      841 caagttgatg cttttacaac tatacctttt ggtggaaatc ctgctggtgt cgttactgat
      901 gcctctaaca tttctgatga aattaagcag aatgtggcta aagaaatgaa tttatcagaa
      961 actgcatttg tttcagagtc aaatgttgca gattttaagg tacaattttt cacaccaggt
     1021 ttagaagttg atttatgtgg tcatgcaaca ataggtactt tccatgcact tttggaagaa
     1081 ggaaggctta atactaataa gagtatattt tatcaagaaa caaaagctgg tgtattatct
     1141 gtagagctta aggaaataaa taatgaaaat atatttatga tggaacagaa tacacctgaa
     1201 tttgaaaatt tagataattg cagaaaagaa cttgcacaaa tgataggtgt gcatgaagat
     1261 agacttcttg aatatcctat tataaaggta aatactggac tttggtggct tgtatttggt
     1321 ctaaaatctt tagatgattt aaaaaatatc tctccagatt taaataaaat caaaaaattt
     1381 agtggtgata ataatataat tggtataact ccattttgta tagaaactat agataaaaag
     1441 tgtgactacc atttaagatc tatagcacca tatgttggag ttacagaaga ccctgtatgt
     1501 ggaactggga acgcttgcgt atcatcttat atagtacatc acaatatttt agataaaact
     1561 agttttattg gtgagcaagg taactttata aacagacctg gaaaggtata tgtaaatata
     1621 actagagaaa acaatgacat aacttctgta aaaattggag gaagtgctta tactatttta
     1681 agtggtgaaa ttatgtatta atcccatacc taataaaaac aaataaaaat actagctaac
     1741 taaagctagt atttttattt gttattttta tttaataatc atactctaac aaatactata
     1801 attatatata tcataatatt gtatttagaa gtaaaatttt atttttatta ttaattagtt
     1861 ataaatcaat ctaatacaac ataattacag tagattaacc tataaaacat aataattgct
     1921 tactaattat ttatcatcct caacaagtct acttaatgtc ttatcagata agaataataa
     1981 tatcgctgca acaaatagta ttactggaat tatagtatat actttaatca tatcaaattt
     2041 aagagtaaat gcatatatat atccatatcc tttaccagct ataaaagtag caaatgtcca
     2101 tactcccata agaacaccta gaagcttctt aggtgcatac ttacttacaa aagagttacc
     2161 aagtggtgag aataacattt caccaagact taaaagaata ccaaacatga ttatccatag
     2221 tatactagct ttagcagttt caggagctcc aactcctctt gaaaattctg caccaacaag
     2281 cattaagaat gcaagaccta agaatattaa tccaagacct gtctttttaa ataaactcat
     2341 atctccttgt ggtctactag caagtttaaa ccataatgct ccaagaactg gtcctaatac
     2401 tatacataca agtgaattta atgagtcaaa ccaagcaaga ggaacatcaa atcctccaac
     2461 attcatgttt acaaatgctg caccataatc ataaactgca agataagtta gataccagaa
     2521 tacccagaaa attacagaga agaatgatat aagtataata gcccaaattc tcttcttatc
     2581 catgcttgta agaggagact tatctttttc ttcaattttt tcagcaacta caccttcttt
     2641 aaaaggtctc tttcctgcat tcccaaggaa tctccatcca tatatgaacc atattgcacc
     2701 tattacacaa agtacagctg ctataaagaa acattgagaa aatccaagta caccattttt
     2761 tgcaaatgtt ttaaggtaaa gaataccaac tgctatagta ccaataaaag aacctatatt
     2821 aacaaaagaa tactgtactg aaaatgctgt atcaagttct tcttgactat caaaaagctg
     2881 tccatttacc gcagatacgt tacctttaaa gaatgcagta ccaattgaaa ctaagattat
     2941 cattgcattt accatagaaa ctgtagttgc aatagaacca agatagtatc ctactcccat
     3001 gataagcata cctataggta tagtatatct agctcctatc catctatctg atatatatcc
     3061 cccaattata ggtgcaaggt atgtgaatgc aacaaggttg gcttgcatta tagcagcttg
     3121 tcctttatct attccaagac caccatgaac tatagctgtt gtaaggaata tatatattaa
     3181 gtactttgaa gcataatatg ctgctctttc aaatgagaaa gttgttgagc agatataaaa
     3241 tcccatagga aatttaccct tcttttttac tgttgagctc ataattttaa atcctttctt
     3301 aattgtattt ttatttttta cgttttgtcc aaattctttc ttttatgaaa gatataatat
     3361 tattattttg taaaacaatt tgtaattatt attaagataa ttgcgcattt atttgtagag
     3421 tacatattct catggaataa aattaataca cttcttaatc ataaatttaa attatataaa
     3481 ttcactattt tcagatatag ttataaatta atatattctc atctaatacc atgaaaaaac
     3541 cttcgccata tgcaattctt attttttacg tgatagtatt ttacaaacta aaatccccct
     3601 tttgtagcaa cttatgtaat tacattttag tgctttttta aaggagtctc gaataaagca
     3661 acatatattt tgttcaaata atgtttttat ttatttttta aatttatata ttacttcacc
     3721 caaccaattc tcaacttaaa taactaaact actatctatt cacagattag gcttatttct
     3781 tacacattag tttataataa acactgtgaa tctaaacata aaaactgtta ttttaaagta
     3841 attacccata tattcgcttt gatactattt attgaggtta tactctttat gtataatata
     3901 ttatttataa aatacctcaa aatatattat tcataaaata cctcaaactg aaaacttttt
     3961 cacgaatatt tatttttcac ctcccctttt ttatttcacc atgtaaatcg taccataaat
     4021 atgcacttat atgcaagaaa aaaatctatt aatcgacagt aaataattag aaaaattcaa
     4081 attatattta tcaattatat tatataaaat atttcctatg aaaatataaa ttaaattaat
     4141 gttcagaata gtttttagat atgaaatctt gat
//
LOCUS       DAEORZ010000228         4149 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEORZ010000228 DAEORZ010000000
VERSION     DAEORZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.234"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     regulatory      complement(154..251)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            477..860
                     /locus_tag="KRH39_003655"
                     /pseudo
     CDS             477..860
                     /locus_tag="KRH39_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      1216..1309
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1392..1877
                     /locus_tag="KRH39_003656"
     CDS             1392..1877
                     /locus_tag="KRH39_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG8513522.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     gene            2233..2631
                     /locus_tag="KRH39_003657"
     CDS             2233..2631
                     /locus_tag="KRH39_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513523.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    3067..3330
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3481..4032
                     /locus_tag="KRH39_003658"
     CDS             3481..4032
                     /locus_tag="KRH39_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBG8513524.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT         1790 a          385 c          598 g         1376 t
ORIGIN      
        1 atcatctaac tgtgtatcta aaaaattaca ataatcatac gaactactaa tataagcagt
       61 caatacatgt gtacatttat ccattttatt tccaaacata taaattatta tccccctaaa
      121 aaactttcat aaaaacaaaa agaacactat ctctttggca actggctgac ataactcata
      181 agatatttat ataagtctta gtccctatag ctttgcgtca ctaaatttct ctagttttgc
      241 cgatttagtt ttattctaca actaaaataa tacaatagaa ttagttatta ttcaacagga
      301 ttgtttgaaa gttaaataaa tttatagata tgaatattat tttacactta aaaacagact
      361 aaacacgtag agtacctggt aaaaattaaa tatagttttt gttctaaaaa gctcctatta
      421 taatttaagc tagataaatt tgaagaagtg acataaattg aaagaaggaa tatattatgc
      481 taaataaaaa attaccagat acagaattaa aaattatgaa atgcatatgg aatactggtt
      541 ataaaactgt aatatcaaaa gatgttgcag atgaaataga aaaaacatat ggttggaagc
      601 atacaactac aattatactt ttagctagat taaccaaaaa aggtttttta atatcataga
      661 gaattgggag gcatgtacac tatacagtat taataaaaga gagagaatat ctcaaaatag
      721 agggaaaaag agtttttaga ggtttacata acaatccttt atcagaatta atttcaaaat
      781 tacatgatgg agaagaaata actgaagaaa aaataataga aataggaagt tggataaaaa
      841 gctggaaata tgaagattag taaataaaag gtagctaata atataagagc tacctttttt
      901 atgtggataa tactggggat aagctgtgga aaacttatat aaaaattgtg gataaagtgt
      961 tttgctgaca ttttactgac aaaagttaaa aaaataaatg ttatatatat tgtaaaatgt
     1021 tgaaaaatag ccatatatat aaaatataag taacactata gataaattac tgtcttttat
     1081 attgactata aagaacagta attgaataaa tataaagata ttttttgatt attattaatt
     1141 aaaaatttaa tttatattta aataatataa aatttgaggt aatattattt tttaattgtt
     1201 caaattatgt tattattcag atataggcaa tattagataa atctaatgac gcaaaactat
     1261 agggactaaa atctttatgt aataaaggtc atgtcagcca gttgccaaaa agataataat
     1321 tctttttgtt aaaagtattt ttagagttat atattttaat aacttaatat ttggcaagag
     1381 gagggcttaa gatgatatct gaattaatgt taaaaaattt tcgcaaatct agcatcaatt
     1441 ttaagactaa aattaaattg aatttaagag aaaaaccaga cataaatagt ttgaaattaa
     1501 aaagtatacc agaaggtaaa attgttaagt taaagtgtgt agatggaata tgggcagaag
     1561 ttgagagtaa ttatgacaaa ggctggttac tttataaata tttagaaaga ttaagtaatg
     1621 ctaaaaacaa tagtaatgat acaataaaaa atagaaaaaa gaattacata ggaaatataa
     1681 gaacaaatgg attaagttta gaacttagaa atgatagaac attagaatct aaagttataa
     1741 ctacaatccc tgatggtttt aaagttgaag tatgttatag cgttggaaaa tgggcaagag
     1801 ttaatattaa taaaaatgaa aaacgatatt ctggatatgt atataatcaa tacatagaag
     1861 agcatttaag tttataatac gatactagca agctttaaat atattaataa atcacaaaaa
     1921 agaatcccat taatgggatt cttttttgta gaaacattat aacagaacta aagagaataa
     1981 attaactaat ttatttttat tataaagtaa ttgtaaatgg taaatttaat tgaaagtaga
     2041 tttaacttat actttataca ccaagtatca taaattaata ctactttgaa aaattttaaa
     2101 aaaagtaaaa gctatgataa taaaaataat attaaagata ataagatata taagaaactt
     2161 gaaaaaattt gtaattttta gttatatata tattataatg aactagttaa aaatttttat
     2221 gagggggaaa atatgaataa gaagaaaaaa ttgattggga tagctatatc ttttctactt
     2281 ataatcagca ttataaatcc attacaagta gaagcagaag ctattattga gtctagtgta
     2341 tacatttctt caataaaaga gaattataca tatgataata ctaaatttaa aactattaaa
     2401 tctaaaagta gacataaaag taggaaatat aataaaagta aagtaaagtt gaaaaaacga
     2461 agtataagaa ctaaaaagta tggatatcaa actataaata aatataaaag acaaaacgca
     2521 agaagacata atatcctaac aaaattaata ttgtccataa tagtcatatt tttggttata
     2581 gttttagtag tgctaatatt tattaaaaga aagaagaaaa aattttacta aaaataatta
     2641 aaagaatatc atttttttaa tcattggtat ctatttaata atatgatgta attaaaatgg
     2701 aataatattc tgtttcaaag gaggcatata aatacaggaa tgagtattaa atgatggtta
     2761 attaagtaaa tttattgata aatgtactta attaactttg aagaatttaa ttcctcatga
     2821 atcaaaatat aaaattacat caacagttat tcacaaaatt aatataagaa gaattagagc
     2881 caaagagcag attattagac cattaaagta attattcata gaattttgta tatgaaaaga
     2941 aatgtatagt ataatttact ttttttataa aaaatagatt acaaaatata ttttttataa
     3001 aaaggtattg acatttttta gaaaaaagaa tatcattatt ttaacaacaa aattcaatac
     3061 aataaattaa atcctatgac aaggaagagt agttaaattt aggttcagta tagagagctg
     3121 aggatggtgg aatcttagca agaaaaaatt tgatgaatgg cccttagagg aataaattga
     3181 aatcatagtt ttgagagtag actttatcgt cagtcgcacg ttatagcgaa taaggtatgt
     3241 aagtacctga taatgagatg tatgaaaatt gaattttata ttaattttta tataatttag
     3301 gtggcaacgc ggataatctc cgtcctatta gtttaggacg gagtttttta ttttactgaa
     3361 aatttaaaag atagaaggtg gtgagaaaga tgttatgaat ttcaaaagct aaagatgatg
     3421 atatagtagg ttggcaaaaa aataaattga aaattaaaaa aataggagga agtacaaaaa
     3481 atgaaaacaa atacaaaaaa attaacttta aatgcaatac ttttagcgat gggattatta
     3541 atacatcaaa tcacaccagc tataggatta ccaatgaagc cagatgtacc actagcaatg
     3601 ttatttgtaa tattggtttt aaatagagat gattataaaa cttgtttgat agcgggtata
     3661 gtaacaggaa tatttacagc acttacatca agttttccgg gaggtcaaat tcctaatgta
     3721 atagataaaa ctcttacagc aaatatagta tttttattaa tgaatattag ttataatatg
     3781 ccttttataa aaaaattagc taagaaaaca caagatactg tagtagttgc aataataatg
     3841 ccaataggaa ctcttgtaag tggaactatc tttttattag cagctcaagc tatagttggt
     3901 cttccagggg cttcatttac agctttattc ttagcagtag tattaccagc tgttttaata
     3961 aatctagttg caggaatatt tttattcaag attgttagtc tatcaataag aagagttagt
     4021 tatcaagctt aaacacgaaa taatatttta ctttataaag cattataata actaaataac
     4081 ataagaataa agtagattat aactatttta ttcttatgtt gtttaaagat ttggtattat
     4141 atatttatt
//
LOCUS       DAEORZ010000229         4104 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEORZ010000229 DAEORZ010000000
VERSION     DAEORZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.235"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            390..1646
                     /locus_tag="KRH39_003659"
     CDS             390..1646
                     /locus_tag="KRH39_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG8513525.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFLLGLIGIPIGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSEVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            1709..2902
                     /locus_tag="KRH39_003660"
     CDS             1709..2902
                     /locus_tag="KRH39_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG8513526.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            3103..3483
                     /locus_tag="KRH39_003661"
     CDS             3103..3483
                     /locus_tag="KRH39_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8513527.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKKFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEQLTEEKIKSLENWVVNWEEAYV"
BASE COUNT         1438 a          420 c          740 g         1506 t
ORIGIN      
        1 aaaatacaat ttacataaaa tagacatatg aaaaataaaa aattatataa taattatggg
       61 aatgaggttc tcccttggat gataatatcc taaactgcca atgagctgat gacttctatg
      121 atatcataga tgccaccagc tttttttgtt gtttaaatcg ataaaatgga ggtgaattac
      181 ttactaaatt tttatttttt aaatttgaag aaatatttaa ttaatagaaa aaatagtaga
      241 ttatatgtta attattttat taagaatggg ttaagagtgt acctatatcc tatcaattta
      301 aaaaacaatt atgatagtca ttacattgac tacagttgat gcaataaaac tttcaaaaaa
      361 tcttatgatg caaatagaaa ggaattaaaa tgatagaaaa atttaaaact ttaatatgga
      421 ttaaaatgat aataagaatt aaaagcgttt ataaaacata tggtggacta tttttgttgg
      481 gactcatagg tataccaatt ggtgcaatta taggtcttat agacaccata tttggaacgg
      541 ttcttctaaa agttacagac attcgagaaa catatccaat gtatctgata ccatttttag
      601 cagtagtagg agttgttatt gcttattgct attttaaatt tggaggaaaa agtagtaaag
      661 gaatgaatct aatatttgag gtagggcatg gagaagaaga aattattcct ttaagattgg
      721 ttccatttat aatatcaggt acatggttaa cccatttatt tggtggtagt gcaggacgag
      781 aaggagttgc tgttcagatt ggtgcaactt tttcacattg ggtaggtaaa aggttgccta
      841 taaaaaatgc ttctagtatt tttctagtaa ctggtatggc agctggtttt gcaggattat
      901 ttgagacacc aatagcagca atactatttg ctatggaggt tttggtagct ggttcactgg
      961 agtatcaatc gctttttcct gcgtttactg cttcttttac agcaagtgca gtatcaaaag
     1021 ctttagggtt agagaaattt tcttttgcac tttcttctga agttgtattt gacttgtcaa
     1081 tattttggaa gcttattgtt ttaggaatta tttttggaat ggttggtgga gcatttgcat
     1141 ggtgtctgaa attgtcaaaa agaaaaatag gtaatagatt aaaaaatcct atgataagga
     1201 ttgctattat tggagtatgt cttagtgtat tatttctttt attttataaa gggagatatt
     1261 caggtttagg gacaaattta attcaaaata gtttctatgg tggagaaatt tattcatttg
     1321 actggctgtt aaagtttata cttacaatac tgactttgtc agcgggtttt caaggaggag
     1381 aggttacacc tttgttttcc attggagcta gtcttggtgt attgttagca ggatttttta
     1441 atttgccaat tgaattagtt gcagcgcttg gctatgctag tgtctttggt agtgctacaa
     1501 atactttttt tgcacctgta tttatagggg cagaagtttt tggatacagc tatttacctt
     1561 atttctttgt agtatgcgct atttcatata tatttaatat ggataaatct atttattcac
     1621 tacaaaaaat ttcaacaaaa caataaataa aacaacataa tgtatgatta gaagagaaag
     1681 aatattagag aaaggatagg gtaggaatat gttgacaagt ttagctttga tttttttatt
     1741 aggaatggcc tcgggaggta tttttaagag gataaagtta cctaacttat tgggaatgtt
     1801 acttacagga attatattag gtccatatgt tttaaatttg attgataatt caatactaga
     1861 tatttcttct gaccttagaa agattgcatt gattattata ttaacaaggg ctggattatc
     1921 tttagatata aacgatttga agaaagtggg aagacctgct gtacttatgt gctttattcc
     1981 agctactttt gaaattattg gaatgatagt tttagcacca aaattactag gcgtttctat
     2041 attggaagca gcagtcatgg gtgcagtagt aggagcagtt tctcctgcaa ttattgtacc
     2101 taaaatgctt aaattaatgg aagaaggtta tggtacagaa aaaagtattc cacagatgct
     2161 tttagcagga acttctatag acgatatttt cgtaattgtg atgtttacag tattcacagg
     2221 attggcacaa ggaaatagta tatcagctat tagtttttta caaattccag tatcaattat
     2281 attaggtgta atagttggag cagtgattgg aatctgttta gcagtatttt ttaagaaggt
     2341 tcatatgaga gattctgcta aaggggtgtt gcttttaagt atctcatttt taatgataag
     2401 tttagagact gctttagagg gtattgtgcc attttctgga ttattagcag ttatgagtat
     2461 tgggattttc cttcagataa aatatcgtgt agttgcaaga agattgtcta taaagtattc
     2521 aaaactatgg gttggcgctg aaattctctt gtttgttttg gtaggggcaa cagtagatat
     2581 aagttatgca tttaaagcag gaattggagc tgtaatttta atatttggag ttcttttatt
     2641 ccgtatggta ggagtattct tctgccttat taagacaaat ttgacaataa aggaaaggtt
     2701 gttctgtatg attggctata ttccaaaggc tacagtacag gcagcaatag gaggtgtacc
     2761 acttgcaatg gggatggcat ctggacaact tattttgacg ttagcagttc tagctatact
     2821 tataactgca cctcttggag cttttggaat tgatgtaact tataaaaaat tattaacttc
     2881 tgtaaaaggc gagagtaagt aaaaataaag attattttaa ctggtaagtt atattttgga
     2941 aaaggattat actattgaat acaatataaa aaaatcattt gggagattaa gaaggtagaa
     3001 gttaagaaca gattttaagg tatgttatga ttattagaat tgattttgtg aaattattat
     3061 tgtaatattt aattatatag ataaagaagc ggagtgataa ctatgtgttt aaaaaaatta
     3121 tcaaaattag agttagttat tatgaaattt atttggaatt tagatataaa aaccaattca
     3181 tatgagatta ttgattatat gaaagaagaa cataatttac cagaaaaagt agctttaaaa
     3241 actttatcaa aactatcaaa gaaaaaattc ttatatgttc aagaaacagg caagtgtatg
     3301 tattatacag ttgcaataaa agaaaaggca tatttagagt ttatatcaag aaatgtgcaa
     3361 aatcttttga agaataattt tataaggaat ttattagtat catttcacga agagcagcta
     3421 acagaggaaa agataaagtc gttagaaaac tgggtagtaa attgggaaga agcttatgta
     3481 taaatgttat tatttttatt acaatttaga taattttggt tttgtaagct ctgcaactat
     3541 ttttagatgg ttgcagagtt tatttttatt ttttattccc ttaagcaata cttaaaagta
     3601 aacgcattta tatttactaa cttcaattac ttctaatact taaattactt tttaaaaata
     3661 ttttgatatg tcatctctat agtaatctag aacttgtttc tattctttat ataaagtttt
     3721 tatgaatgat atatataata ttcgttcatt tgattaaaaa taacaaaata ttaaataatt
     3781 ctactctgat agtttgttaa aaaaataata aaaaatatta ataaacaaaa aaaattatct
     3841 taagagagga gaatgttcta aaatataaaa aggtttctag atttcataaa agatactatt
     3901 ttagtcttga aaatatttag tttgaaaaga ttttaattta atgattgatt aagttaaaaa
     3961 tgtgtatgta ataaatttta ttttatttat tgactaaatt ataaagttta cataattatt
     4021 taataattat gtaattgtta cttaaaaatt gatctatttt aaaatctagt tataacttca
     4081 aagaagactg aaaattaaga aaaa
//
LOCUS       DAEORZ010000230         4100 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEORZ010000230 DAEORZ010000000
VERSION     DAEORZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.236"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(83..1240)
                     /locus_tag="KRH39_003662"
     CDS             complement(83..1240)
                     /locus_tag="KRH39_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBG8513528.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            1394..1963
                     /locus_tag="KRH39_003663"
     CDS             1394..1963
                     /locus_tag="KRH39_003663"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBG8513529.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            complement(2145..2561)
                     /locus_tag="KRH39_003664"
     CDS             complement(2145..2561)
                     /locus_tag="KRH39_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513530.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(2787..3881)
                     /locus_tag="KRH39_003665"
     CDS             complement(2787..3881)
                     /locus_tag="KRH39_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513531.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
BASE COUNT         1406 a          737 c          436 g         1521 t
ORIGIN      
        1 ctcatgaact aaaaagttca ttttgagaca tctcttttat aatgttcttt attttatagt
       61 ataagtttat aaaaattaaa tcctatttat caatctgttc tctaagccac ttagttgcta
      121 catctaactg tttattttca tctttttctt caatagtagg ctttatacct ttttcattga
      181 tataatctcc attaggtgta aaatattgag cagtagttaa cttgtatcct gttccatcct
      241 tcaatcttac aactgattgt accaaccctt taccaaaagt tgtagtccct acagaaatac
      301 cttctttatt atcaacgatt gcagctgtta atatttcagc agcagaagca ctttctccat
      361 tagttaacac tactattgga agtcccaatt tttctttatc agatttcaag tactcagtat
      421 ttcccttatt atcttttgta tatactatag ttccttcacc aattaactca tcagcaactt
      481 ctttacatat atctagcatt ccaccaggat tatttctaag gtctattact aatcccttga
      541 tattttttgc ttttaattct tttaaagctt tatcaaagtc agtatatgta cttgatataa
      601 attgggtaat ttctatatat cctaaatcat tatcaattac tttactttct atactcttct
      661 caattatctg gtctgttttt actttaaagt tcaattgttt atcttctctt aatatagtca
      721 gttcaactgt tttacctttt ttacccttca tcatacgaac agcttcatct gaatttttat
      781 atgagacaga ttttccattg acttttacta atttatcccc tgattttaca cctgctttct
      841 ctgctggaga atctttcatg ggaactacta ctaagccacc atcttttgat gcccctatat
      901 acatacctac accaacataa gaaccttcac tatcattaat tagattctcc atttcatctt
      961 tagtataata accagaatac ttatcatttg ttcctagaaa tagacctttt gctgctccat
     1021 ttaccaaatc agtatctgat acttttttat aaaaatcttg ttttataata gattctagtc
     1081 ctaacaactt atcatatttt ttatagtctt catagagttc tttcgatatt acaactttat
     1141 tccccaatgt caactgaaat gctgatgtaa ccatagctgt tataattact aaaatcaccc
     1201 ctaaaaaaat agctttcttc tttgaaatca caagatacat ctccttttac ataaactatt
     1261 attattatat gactagctgt tatttttatt acatttaccc aaatatattg ctttattaca
     1321 ataaattata ctaaaattta tattaaatac aattgctgta cacattataa catattaagg
     1381 gaggaattat tttatgaaaa aagaatttag aaaaaaagtt attgaatcaa gaaaaaataa
     1441 gagtgcaaac ttcgtactta gtaattcaaa tttaatcact gaaaaattac ttcaaatgga
     1501 agatataaga aatgcaacta atataatgct ttatctaaat tttaacaatg aagttcaaac
     1561 agataattta atcaaaaaac ttttgtctct aaagaaaata gtatcaagtc ccatcacaat
     1621 aaaagaaaat catacattaa tccctactca aataacagat ttaaaagagg gattgagaat
     1681 tggagcatat ggaataagag aacctaacga aaaatcacct acaattgata ttaaggcctt
     1741 agatgttgta attgtaccag ctgtagcata tgatatacat tgctatagat taggatatgg
     1801 tggaggattt tatgatagat ttttagaaaa cttaagaaaa gatgctataa ctatcggcat
     1861 agcatttgac ttccagatat tcgattctat tccaaaagaa actcatgatg cacaattaga
     1921 ctacatagtt actgaaacta gaattatcac tccaaataat tagaatatta agtatttcca
     1981 gaccccattt aatgtagtaa taaatactac tgttaatatc ttactttata agcattttaa
     2041 gtcatggctt ttacaacaat aagaaccata tacaatttac ttacattaat aaaaaccatg
     2101 actttattgt acaatatgca gttatttaat tataaaatac cttcttattt cgtcgcattt
     2161 aattctattc tatctggaag ttcactcata tttgtttcaa caactatgta tggaaaagaa
     2221 tcttccttat ctttatactt tatcttgact ctcaaaacat cctttccttc ctcaaccaca
     2281 ctttctattt tgtccatttc tataccatat ttattatttc ctctagtcac tataatgtat
     2341 attttgttat cgattttagc cgctaatgct ctttcttcat ttgtatactt atccatcaca
     2401 tctaatatct tttctggtat tgaatttctt tgtaatactg tataatctat gggtttagat
     2461 ttctctacaa acatacttgg tatgaatata aatcccccta taacgaatac aagagctata
     2521 actccataaa ttataccttt aatattaaac tttacattca atcttatcac tcctcccata
     2581 tattctaaat atatgattta taaacttatc ttagactcta aattattgat tagtatatat
     2641 ttatacataa ttataaatat atgtaatgta actaaaaaac atgttttttc tatttcataa
     2701 taaacgagct aaaaaataaa aagagctata tgaaaacatt ttcattgttt tcatatagct
     2761 cttgagttta caatattaaa aaatacttat ttgtcactga ttccttcttg atatccagat
     2821 gtttttgtaa tctgtctata aagaacacaa accagtatta tgacgcccat atatccagta
     2881 tatggatata aaataccaac cagcttatca aatggtagga agccacctat aagacctaat
     2941 atagctgcta ttaaagttat taatttaaac ttaggatgtt tatcatctac aaatctatta
     3001 gttactgacc ataataaagg tacagctgtt gtatatatac ctaatataag aattattgag
     3061 aataaaactc caattactgg tgaaatctta tctgctaaga ataatgatgg tattgctttt
     3121 gaatataagt ttcctatatc agaaagaata gctaagtgca tcattatagc tgcagccata
     3181 agtgctactc cacctaatat accaccacaa atagcttctt ttttgttagc agcacttgct
     3241 cctaatccag ttaagaatgc cataacaaca attgtgttat aagtagtata tattacacca
     3301 gatagatagg catttggaac tggtttagct atctttacag tgctaagaac ttgtgcagct
     3361 tctcctatat tacctatgtt tttagctaaa cttataccac caactaaaag tgtaaagatt
     3421 attattatag gccctattcc acctagtatc tttgataatt tatcaagacc tagtgaaaca
     3481 gttaataatg ctactactgc catacctact ctacctacaa atggatttaa tccatagtat
     3541 tcagttaaag ttgctcctgc tcctgaaatc attactacga aaacaccaaa taagaataaa
     3601 ggtccaaacc attcaaagaa tattcctaaa tacttaccac aataaacacg ataaattttg
     3661 ataggttctt taagttgaag ctctttacct tttgacataa ctgaagcacc catccatgag
     3721 aataatacca ttgaaattat cccaccaata atacctgcaa aaccataaaa agagaagaat
     3781 tgcattattt cctgtcctgt tgcaaatcca gaaccgatta ctgttgctac atacgcacct
     3841 gcgaaactga tgactgtttt aacatttact ttttctgaca tatctcagtt acctccccat
     3901 gtgaaatttt ttcttctgat taaacaattc gataaactac ctacaatttc ctttaacata
     3961 aaatacatga taactgtttt atagcttttt agtataataa cttaaaactt ataatctgat
     4021 tttataccct actcctcctc tcataatttt tttagattct tttatctaag tagtcattat
     4081 atataaaatt agtttctcta
//
LOCUS       DAEORZ010000231         3959 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEORZ010000231 DAEORZ010000000
VERSION     DAEORZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.237"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(101..1786)
                     /locus_tag="KRH39_003666"
     CDS             complement(101..1786)
                     /locus_tag="KRH39_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513532.1"
                     /translation="MKYLLEIAKKEKLLVIIYITLGISIELLNSFSANYYQNLIDRFN
                     NGTISFCIIFIYGAVLITLSLLRYIDEYPGRKLEHSFFLDLKLKALEKMSKIDYLEYQ
                     KLGTGKLIQKIENGANAGRNIFFNFLLVVVRKIIPSILFSMVFIYRIDKKVMFTILIG
                     YFIVFIITNLLIKALYQIKEHILINEERMNHFLVRGFVEMVVFRVNKRFKYEIRQSES
                     AKKKIIDSKVKMTLIHEAFFTIFSLIVTFIKIVTIVYGWKTKTISIGSIIALITLLDN
                     AYTPIAIFNVLYIQFKLDKAAYKRYTDILELNNDGQLLAGKEVNLMYGNIEFNEISFL
                     YNNKREIFNKLSLSIKSGEKVAIVGESGSGKSTLIKLLIGLLKPSEGAIFIDDYNLLE
                     MNLNSYYNYVTYVSQDSPIFDGTLRENIVFGKTIKSNDIIEVLEKVGLKNLYLKLEAG
                     LDTKLGEKGTTLSGGEKQRLALARLWFSDAKIIILDEATSALDNITEEKVMLNVMEFL
                     KDKTTIMIAHRFNSIKNVENIIVLKEGSIIGSGSFKKLLDDNLYFRQLYYTMS"
     gene            complement(2717..3298)
                     /locus_tag="KRH39_003667"
     CDS             complement(2717..3298)
                     /locus_tag="KRH39_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8513533.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRSFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(3291..3521)
                     /locus_tag="KRH39_003668"
     CDS             complement(3291..3521)
                     /locus_tag="KRH39_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8513534.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
BASE COUNT         1453 a          669 c          330 g         1507 t
ORIGIN      
        1 actaaaaaat attactggaa tctcaaattt ttaataatat tccagtaata tttaaataat
       61 attaaattaa gaaagatatc ttcttatact tacataataa ttatgacatt gtatagtata
      121 attgtctaaa atataaatta tcatctaata gttttttaaa gctaccacta cctattatac
      181 tcccctcttt aagaacaatt atattttcaa cattcttaat tgagttaaat ctgtgagcaa
      241 tcataatagt tgttttatct ttcaaaaatt ccataacatt tagcataact ttttcctctg
      301 taatattgtc taaagctgat gtagcttcat ctaatataat tatcttagca tctgaaaacc
      361 aaagccttgc taatgctagc ctctgctttt ctccacctga taatgtagtt cctttttcac
      421 ctaatttagt atctaaacca gcttctaatt ttaagtataa attttttaaa cctacttttt
      481 ctaagacttc aataatatca ttacttttta tagtttttcc aaatacaata ttctcacgta
      541 aagttccatc aaatataggt gaatcttgtg acacatatgt aacataatta tagtaactat
      601 ttagattcat ctcaagtaga ttataatcat ctataaatat agcgccttcg ctgggcttta
      661 ataatccaat taatagttta attaaagttg atttaccaga accactctct ccaacaatag
      721 ctactttttc tccactttta atacttaaac taagtttatt aaatatctct cgcttattat
      781 tatacaagaa gctaatctca ttaaattcaa tatttccata cattaaatta acttctttac
      841 ctgctaacag ttgaccatca ttattaagtt ccaaaatatc tgtatagcgt ttatatgctg
      901 ctttatctag tttaaattga atatacaata cattaaaaat agctatagga gtatatgcat
      961 tatctagtaa agttatcaat gcaattatag agcctatact tattgtttta gtcttccaac
     1021 cataaactat tgttactatt tttataaaag tcactattaa tgaaaaaatt gtgaagaaag
     1081 cttcatgaat caatgtcatt ttaaccttag aatcaataat tttctttttt gcagattctg
     1141 actgacgaat ttcatattta aatcttttat ttactcgaaa tacaaccatt tctacaaatc
     1201 cacgaactaa aaaatgattc atcctctctt cattaattaa tatatgctct ttgatttgat
     1261 atagagcttt tattagtaaa ttagtaataa taaaaactat aaaatatcca attaatatgg
     1321 taaacataac ttttttatca attctataaa taaaaaccat actaaataaa attgatggaa
     1381 ttatttttct aacaacgact aataaaaaat taaaaaatat atttctgcca gcattagctc
     1441 cattttcaat tttttggatc aattttcctg tacctagctt ttgatattct aagtaatcta
     1501 ttttactcat tttttctaaa gcctttaatt ttaaatctaa aaaaaaacta tgttctaatt
     1561 tcctacctgg atattcatca atatacctta aaagagataa ggtaattaat acagcaccat
     1621 aaataaaaat tatacaaaaa cttatagtcc cattattaaa tctgtctatt agattctgat
     1681 aataattggc actaaaacta tttaaaagtt ctatacttat gcctaatgtt atatatataa
     1741 taactagtaa cttctctttt tttgctatct ctaataaata tttcataact gtacacctat
     1801 ccttatttat tttactttat taacttaaaa taattttata gataaaatgt ttctttgttt
     1861 gtttaaattt agtgtgatta ttaatcattt caagttaaca aaacctaaag tttttagcag
     1921 tacagttcta tttgttagca aattattatt ttactcagta cattatgcta acatataatc
     1981 taactgtact gagtaaatat ctcgtttcat catataaaat ctccttttta tagtttaata
     2041 tatatagtta aatttactat ataatctgaa tatttatttt tctatcacct ttaccagaaa
     2101 taaaatcaat tatttattgt atacgcaaaa cttcataata ttttcaacat ataattatta
     2161 tacagaactc ctaagttaat ttaaacttag aatttatttt tataagaaat gtcattgacc
     2221 tacctttaat aaaacagcca ccattttttt atgtattaac atataatata tagtaatgaa
     2281 aacatattaa ttaaaaaata ttgtataaat tagtttatct gtaacaaatc cagtttatgt
     2341 ttcaacaagc acgtaagact tcttgaaatt taatctgctt cctttaaata tctattactt
     2401 gttttaagca taagtttaca atcttctata acatttatca tttcttcaga tttctctgac
     2461 gtacttaaaa tacaagttgt tacattgttt ttctatttaa ttagtaatgt agcatgtaaa
     2521 aaagaagcat aaatgagttt actttataca aacttcattt atgctccact catataccta
     2581 tcatagctaa tagaaactat tactcatctt aaatctattt catatttaga tatttagata
     2641 tttatatctt tcttattttc ttaaatcaac tctagattta aacattaaac cccttaaatc
     2701 ttcacaaaat ctcttcctat acagacttac tctttgcctt acatatcatc tgagtcatag
     2761 tacccatagg gcaataaaca caccatgaac gaggtttaaa caatatcata gtaatcaaac
     2821 caagaacagt tgatgtaagc ataacactat aaaaaccaaa tgcaaactgt accacccaag
     2881 gtgaaactaa agttccatga tacgcccatt gccaaggcat ttttatagtc cacaacaaag
     2941 tcactacttc tttaaaactt ctacttcctt caaacactaa ataagttgaa aaaagcatat
     3001 tagcaaacat taccataaaa aatgtcaaaa acccatatct aaaccactta cttctaataa
     3061 atttaggaat atctctattt cttgacagct tcaatttatt tccaagcaaa ttaaacagtt
     3121 gccctcttcc acaatactta ttacagtaag ctttatttcc cttaacaata gaaatagcta
     3181 gtggaacaaa gaaacaaatt agcccaatcc aagcgaaaat aatatttaca aatcccaata
     3241 tcaaatatat tgaagaaaaa atccataaat agtcatacca atgtttttta ttattcatga
     3301 cacttacctt cttcctcttt taatattgtt ataatctcaa ttacagatgc tggacatatc
     3361 ttttcacaaa tcccacatcc aacacataaa tctctattaa tttttgcact aatgccacat
     3421 ggaactgaga tagcacttct tggacatgct tttatacagc taccacaagc aacgcactca
     3481 tttgctaaaa tatttgcata cttctttttt ttattagaca taataacctc cctatattat
     3541 taatctataa gaattatatc aagatactta ctttttatct gtgacttaat cacataaaac
     3601 ctcaatatta aaaatcacac tctaaataca aaggagtttc aaataaaaaa cgatatgaag
     3661 tcattaagtt tagcttcata tcgccttttc ttttgtgtat ctatataaat atttattatc
     3721 taatatttaa tatatctata atattttctt atacagctct ataattttac acctaatcat
     3781 attctattag taacaggtta atacccatct aaaactataa ctttattcac aatcttattc
     3841 tattagccac aagttaaact catattgaag tttatcttta gttacactct tacgctatta
     3901 atcacaagtt aaactcatat tgaagtttat ctttagttac accttactct attaaccac
//
LOCUS       DAEORZ010000232         3920 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEORZ010000232 DAEORZ010000000
VERSION     DAEORZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.238"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            165..1670
                     /locus_tag="KRH39_003669"
     CDS             165..1670
                     /locus_tag="KRH39_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG8513535.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1784..2482
                     /locus_tag="KRH39_003670"
     CDS             1784..2482
                     /locus_tag="KRH39_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG8513536.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            2767..3855
                     /locus_tag="KRH39_003671"
     CDS             2767..3855
                     /locus_tag="KRH39_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513537.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTAVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT         1542 a          392 c          637 g         1349 t
ORIGIN      
        1 atacaataat aatagaagta ataaaaaaaa ataattaata taaaaatgtg tacaaactaa
       61 tgaatattga tatagtaagc tatcatatga aagagtatgt acacattttt tttactaatt
      121 tttcacaata tgaacataaa taatttacag gaaaggaatc ttttatgaaa attcaaaata
      181 ctgagtgggg atatattgaa tggaaacata cttatgatga aaataatcca aaacaagcta
      241 tgaatatata cattgcagta actatgcctg gtaagaaaca ttttaatcat gttcattatg
      301 gtcaagagca gatgatatat attttagagg gagagggttt gtatattata aatggagttt
      361 ggaagccatt ttatcaaggt atgatttttt atatagaatc tggtagcaca catgaaacta
      421 taaatacagg cgatagagaa ataaaagaac ttattgtttc aaataatgta gatgatgtag
      481 gagagagtga agttatagat attaatccta ataattattt aaagaagacc ttgataaatt
      541 atagtgaaag tacattaaat ttgtatgcag cagtagaatc aattcgtggt caatttatag
      601 acccgtttaa aattccatta attatatatg atgattcatg gaatatagtt ttaaaaaatc
      661 cttattttcc acttttttgt tttgaaaaat gtaatcctat gaaatttcct caaaattgtg
      721 attgtatgaa tcaaaaaagt agcaatcaat ttgtatgtga atatgggata actatatata
      781 atataccaat actgtacaaa agcaatagca taggtgttat aagaggaggg tatgtactat
      841 tatctgattt gaatttagat acagagcata ataatttata tgatattcca gagggagctg
      901 ctagaagtat aaaacgcctc ctgaaacaaa tatctaaaaa tataataaat ttttgctctt
      961 ttaatgatat aagaaaggat ttacaagaaa aagagaagac tatagcaaga acatatcatt
     1021 atggagagca acttgaaatg aatttaaaag tagctcaaga tatggttact aatttaagaa
     1081 taaatcatca ctttttattt aatacattaa attcaatggc aagcatagca ctagatgatg
     1141 gtagttatga tttgtatagt gcaataatag atttatcaag aatgtttaga tatacaatga
     1201 ggtctgattt aagatttgta gaattagaat ctgaaatatt atacattaaa aattatttga
     1261 atttacagaa attacgttat ggagatgcac ttaaagtaaa atatcttatt ccagagaagt
     1321 tatataacct ttctgtgcct tttaacttta ttcaacctat tgtagagaat gcatttacac
     1381 atggatttag agatattgac acagaaaagc gaattgaaat aattgcaaga ttagattctc
     1441 agtatgctat tatagagatt cataataatg gaactatctt agatggaaat aatatagaca
     1501 aaataaaggc aggtattcgt agtaataatg gtcatggttt gtctttaata tatacaaaat
     1561 tcacatctgc ttatggaaat aattttgata tggatataaa gtcgagtgat aatgaaggaa
     1621 cttacataat gattaaaatt cctattgaaa attataagga gtatgtatag aacttgtgaa
     1681 aaattaataa gttttataaa tgttaagaaa tatattagat gtttttatgt tgttttatat
     1741 atcttacttt tttatattat taagctaaaa gggaggtatt ctcatgagaa gagcactaat
     1801 aatagatgat gaagaagctg ttctaaaaat aataacacat tttatagaaa tgaaagatat
     1861 gccaataaaa attgttggta aagcaacatc tggtgatgaa gcagtagata aaatcattgg
     1921 tttagagcca gacatagttt ttatagatat tcaaatgcca atatacaacg ggttagaagt
     1981 aatagaaaaa acatctcatt tatctaaaaa aataaacttt gtagtaataa ctgcatttaa
     2041 ctattttgaa tatgctcaaa aagctttgag attaaatgta aaagatatac tccttaaacc
     2101 attggatatg aaagaattta ctaagtctgt agaaaaaatt attggatatc aatataccaa
     2161 taatgattta ttaaatgaga tactggaata tataaatcta aactatagca atgacttaaa
     2221 gttaaatgat tgtgctagat tatttttaac caatccatct aatatatcta ggatttttaa
     2281 aagtaattta aatacaactt ttatatcata tctaaatcat ataagaatag aaaaatctat
     2341 tgaattactt gaaaatacta caaaaagtat aaatgagatt gcagaaatag ttggatataa
     2401 tagcctaaat aacttttata aaaactttaa gatagaaaaa ggaatgactc caaaagttta
     2461 taaactgaac actaaaaatt gaaattgata aataaaaagt caatatagat aattgtttga
     2521 gtgtgtctga ataagtatac tttaattatg ataaaaatta aagcgcttta aacatattta
     2581 ataatattaa accttataaa agctgtacct atttatataa aaaaatatat ataaataaaa
     2641 agaaaaagat atacaaaata aaaaaagtga attaaatact gtttcaaaaa gacagaattt
     2701 tcatttaatt cactgagagt ttatatttct taaaggaagg taactttata atggaggtaa
     2761 tttaatatgt cagacaaagt gaatatgaaa aatattatct gtattgcagg cgcttatcta
     2821 gcaacagcaa ttggttctgg gtttgctact ggtcaagaaa ttctacaatt ttttgcctca
     2881 caagggtata tgggtttaat tggagcatta atatcaatgg tattgttttc atttcttggt
     2941 gctgaagtta taactagagg tagagaatta aaacttaaag aaccaacaag aatttacact
     3001 ttttattgtg ggaagatttt aggaaaattt tacgaatggt ttagtccaat atttttattt
     3061 gggattctta tagtaatgat ttcaggtgca ggctcaactt taactgaata ctatggtata
     3121 aatccttact taggaagagt attgatggct gttgctactt taattacagt gtctatgggt
     3181 ttagaaaaat tagtaagcat acttggacgt attggaccag tgatagcagt atttacaatt
     3241 gctgttggat taataagctt gtttaagcat tttgatggtt tatctatggt tggagaagct
     3301 atgaaaaata ttgaagttac aaaagcatca gaaagtccaa tcgtttctgg tgtaatatat
     3361 aatacattaa atattattgt aatgattgtt tttttaacag gaatgggagc ttctattgaa
     3421 agcaaaaagg atgctttttg gggaggttta ttaggtggaa tagtgtttat gactgctggt
     3481 atagttatgt atttggctat gatttcagat ataggaaatt tgtatatgaa agaaatacca
     3541 tcactttatt tagcagataa tatatctccg attgtaggtg tatgtttttc agttgcatta
     3601 atattaggta tctatacaac agctgttcca cttttatggt ctgtaaccaa tagaattgtt
     3661 gaagatgaac atcctaaatt taaattggta acagcagtaa tagctatttt ggcttgtata
     3721 ggaggttttt taccgtttgg taaattagta aacattttat atccatatac aggttatatg
     3781 gggcttttaa ttttagtctg tatggtgtat aaagcaatta catgtagaaa aaaatcaaca
     3841 caaagtcagg aatagagcca cttattaata gtttttattt aaagaaattt tttatataat
     3901 ccgaatttaa aaagtcaaaa
//
LOCUS       DAEORZ010000233         3901 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEORZ010000233 DAEORZ010000000
VERSION     DAEORZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.239"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(223..633)
                     /locus_tag="KRH39_003672"
     CDS             complement(223..633)
                     /locus_tag="KRH39_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513538.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(849..2231)
                     /locus_tag="KRH39_003673"
     CDS             complement(849..2231)
                     /locus_tag="KRH39_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG8513539.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2242..2601)
                     /locus_tag="KRH39_003674"
     CDS             complement(2242..2601)
                     /locus_tag="KRH39_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8513540.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(2806..3585)
                     /locus_tag="KRH39_003675"
     CDS             complement(2806..3585)
                     /locus_tag="KRH39_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBG8513541.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         1393 a          645 c          377 g         1486 t
ORIGIN      
        1 agtcttattt attttattct cactccatat gttcaattac atttgtctaa attctttgat
       61 acttttatta cataatttta acaagcttaa gtgttatttg ggtagtgcag ttgataatta
      121 tttaaataat catcaactgc atgtttatat cattacagta gcttaatgta tttaatcata
      181 cttacttagg attttgaaat atcttagaga ttatatttaa cattagtcaa ttgctgcata
      241 attgtaagca attgaaattt gagacccatc ttcattttct atagtgatag gcttcataat
      301 ttttaatcct tcagttttta tcttttctaa ttcttgttcc attttcttaa ttgtttcctc
      361 aaggtctttt tgacttacat ctttgactgc attagggtct ttagcatagt cttttaattc
      421 ttgaatatgt ttagcatact cttcataagt ataatgctca acaccatact tttccaattc
      481 agtagggtca gttattcctg attttattag agattcaaat ttaggtaaag caatattaga
      541 tgaatcattt ccatttgcaa atactgctcc agttcctcct agtaccattg cagataccat
      601 cattgcactt ataagggatt tttttatttt cataaccaat cttcctttct tagagattta
      661 tttgggtagt gtagtttaaa atgactattg caatagttat caaccacatt cttagactat
      721 cacagtagtc atgtttatgt caagcctttt taaaaaactt ttcaataaaa aatactatct
      781 caaaacaaaa ttaattattt tgagatagta ttttaattct aaatctttta tattactaag
      841 atttatggtt actctatcca tgttctatat tgttctttat taactgctaa caaattccct
      901 tttcttgata tgacaaagcg attatcatta tagccacttc gctcaattga aacttctcct
      961 gaatcagtat aaagtacatc cttactatta ctagatttat ttacatacgt ttcttcacta
     1021 ttctcagaat tagttacttt gctcatagat gattcttcat gctctctaaa ttcaattaca
     1081 gatacttttg ccgtactaaa atgagtctct ccatcacttt tagctttact aacaagaata
     1141 tcagattcta ctttgggaga atataaatca gttgatttac tacttattga aaatccaatt
     1201 ttatcacttt gttcattttt tgttaaacca aaagattcaa cctcattaat ctctttattt
     1261 tctgttgcaa aaacaagacc tgtcccacaa actaatacag ttgaaactaa aaaagtaaca
     1321 taagttgatt ttttaaattt cataatatta actattctct tttctatttt tgtctttcca
     1381 aatccactac atattgtcat tgaaaatgga ttattctcct ctgctagact tattagtgtg
     1441 gttgcataat cagccctata ttcttcacca agtatactca taaccttttc atcacaacta
     1501 aattctatgt ctttatttag taaaaaaaac atcacccaga ctaagggatt aaaccaatgg
     1561 acaacaagag ctacaagcat aaataacttt aacagattat caaagctatt aatatgaacc
     1621 atttcatgag ttaaaacaaa gtctaaagtt tgattattag cataattcat ttgctttggc
     1681 aaaataattc gtggttttaa aataccagat gtaagtggtg tcctagtaaa ttcagatatg
     1741 caaacaataa atcttctaaa acggctattc ttacacatcc attttttaat gaactcatga
     1801 ttttttatat gaattgaagt tttaaaaaga ttaattattt taaaatatct aaagcataaa
     1861 agtatgctaa acataatagt aactataaaa gacaaaatgc ccaacatttc caacatcata
     1921 atattttcaa tattagacat tatttcaaat tcgtcagaat tgtttttacc tgtagagtca
     1981 ctagctttct taagagtata attttctgtt gatgtattac ccaattccac atcaaaagtc
     2041 gattcaatta tactataaat actaaaacca gaatttaaac ttactggtat tagaagttta
     2101 aataaaacaa tcaaccacaa taatacaaat acctttttag gtaatttttt aaataaacat
     2161 gctcttataa aaataactat taaaagtaat actccagaag taaaactcat ttctaacaat
     2221 ttaatgaaca taaatcttac ctcactttaa tttttcaaca atttgtttaa gttttgtaat
     2281 ctcatcactt gaaagatttt ctttattaat aaaagctgaa agaaattttt ctgaagaccc
     2341 tccaaacatt ttattaatta aagtttctgt ttcctgtttt tgtacttcct ctttgcttat
     2401 taaagcttta caaacaaaat tcggctcaat tctctctatt gcaccttttg acactaattt
     2461 ttttataacg gtatacgtag tgtttctatt ccatcctgtt tcttctttta aaatttttac
     2521 taactctcca gccttgcttt ctcctctctc ccatagaact tccattactt taagttctga
     2581 atcaaataat tttatctcca tataaaatcg ctcctcaaat atagttatca caatagtcat
     2641 tactataata aaaaactatc atagtagtct atactatgtc aatcatttat tttttaataa
     2701 taaaaactgt ctttttaaaa aattcttata aaagacagtt ttatcaatgt tatactttaa
     2761 tgtttaatta aagccatatt ttatttaaat atacaactat ctatcttagt acatagtata
     2821 agatttttct ttatctaata gtatatcctc tacatctggg ttttctttta ctaattgtct
     2881 ataaaaatta caattagtaa cacccacata tggaattaac catagtagac ctattccaag
     2941 agtagctatt gaaagtatat accatcctat aaaactaaag tttagtacaa ataatttaag
     3001 tacatttcct ttcatcatat ctaaactagc acccattgat ttaaatacac ccatattatc
     3061 cttgtcttca caaattaaga aagtagcttg agaaacatat aatagcaata tataaactgg
     3121 taacataaga acaagtatta taagaactag agttattcca gtagaaataa tagtccctat
     3181 tccaacatta tcacttgata aatattcaat tgctccacta gtaagtaaaa ctccaaatga
     3241 taaaaacgct cctataattg tgattataaa agaataaata taaacaataa ttgattcaaa
     3301 taaataaaaa ccaaaacttc ttaaataaat tctcccactt acaaagatat ctcctatctt
     3361 gactttttca tctttagtaa ttttcaagaa catacatgta aatcctgctg aaacgcaaaa
     3421 agctattaca gccactacaa tagtagtcaa tggttcaaaa ggtaatccta caattgatat
     3481 taatactatc aatgcaatat atactgccat agaaattgca catatccccc agtttccaga
     3541 taactgagct ttagatactc gttttaattc ttgccttgat accataaaaa acaccccttt
     3601 cattttataa aaaaactttt aaaaatataa ataatctttt aacgtataat attaatatat
     3661 catattttcg actatataag atacaattat tcaccatgtt ttatcaagat atgccataat
     3721 tgtattacta gttttatata gtcaacatta ccaaatacat caaataaatt tgattaatta
     3781 ttcaacaagt aataaaataa tttctattaa ttatacttta tttagtaaac aaattattta
     3841 ataaaaaaat tacctaatac agtaaattaa ttggtaagat aaattattta ttattagtaa
     3901 a
//
LOCUS       DAEORZ010000234         3871 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEORZ010000234 DAEORZ010000000
VERSION     DAEORZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.240"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(103..639)
                     /locus_tag="KRH39_003676"
     CDS             complement(103..639)
                     /locus_tag="KRH39_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBG8513542.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(657..1970)
                     /locus_tag="KRH39_003677"
     CDS             complement(657..1970)
                     /locus_tag="KRH39_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBG8513543.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2325..2765
                     /locus_tag="KRH39_003678"
     CDS             2325..2765
                     /locus_tag="KRH39_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG8513544.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2950..3138)
                     /locus_tag="KRH39_003679"
     CDS             complement(2950..3138)
                     /locus_tag="KRH39_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG8513545.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
BASE COUNT         1343 a          660 c          379 g         1489 t
ORIGIN      
        1 gaataggata attttcctac tcttttttct gtacttacat ataatattaa aactctttat
       61 tttttaatat atattttaaa ttttcttttt taataaatct atttaatttt cttcttctat
      121 atcagagcca aagggaatcc atgcattacc aaactcttta tctttaaata aataagctaa
      181 tccagttatt tgcttcaatc taagaatttc atcaacttcc atacccaggt gctttgatat
      241 ccattcatca gattttccaa tttcacaaag ttcagttata attttactca ttaaatccac
      301 attatgagaa cctcttgcac gattgtgtcg aatagtactt gccattcttt catcaagaga
      361 tttatcaatt actgaaacag gaagaagtcc gttttctcta tcatatatat cctcattttc
      421 aagcattact ctatatctat gaaaaccatc tacaataata tacttatcct tttcctcatt
      481 ataataacag acaattggca ttgtataccc atcttctttt atacttttat acaacaactg
      541 catttctggt ggggctactg catttggatt atagtcgtta gattctatct tttcgattgg
      601 cactgcgatg attccatata caggactttt atattccata gcatcttaac tcctatctaa
      661 tgaatttttt catatttctt cttaatatat tccgttctaa ttctctgttc ttttgtagga
      721 ccaaatccca taaatctaca catatggtca ttttttaaaa tacaataaca cattcttttc
      781 caacttggta tatctttggt aaatttaata tcatccgtat catcaggtat atcatccaaa
      841 aatatgactc tactcttttt atcttttgta tgatttgaaa ctccatttaa ttctatttta
      901 taaccttttc tcaacaattc acgtataact atttctgaca aaccaccacc tgtattctgc
      961 caaaactcaa tagatgttga aaatttttta atatagctat tccttatatt ttcaggtaag
     1021 gtatcaagca aaaaatatgt gaaacttttc catgtgtgtc catatggcaa tttaacacta
     1081 cgatatccca tagcttttgt tttgccataa atagaagtaa aattaactcc ttgtactcta
     1141 gcaaccaatt tcacccatac atctgggtcc aatattctgt ataaatttaa actctctttt
     1201 gctgaatcat taaaaggtga tgccactctc atttttcttg gagctactcc agctttatag
     1261 aataagtcat aaattttatt atagtcatag tcaaatttat agtgtgctaa ccatatatct
     1321 tctatttccc aatcatatat aggagatgcc gtccagacat ttttaaattc cttagttatc
     1381 cagcactttc ctttatatcc atgctgttta ttcacaatag aacaataacg ttgtagagat
     1441 tctgctgctc gtgttcctaa taagcaaaca acttttgcac cttcatgtat atctctaatc
     1501 catctaccaa attgttttgc caaactttct tgtctcattt tatatttata ataagtaaaa
     1561 ggattatttt tttgatttat tatgtattct ttgactggca tttctcttac ccataactct
     1621 tctttctcat catcccatgg ataccaatac atttcataat ttcctacagc agttcttgta
     1681 gccattggta aacacaccca atatggttca atatcttcaa tattatcttc aaatactttt
     1741 tcaacatatt cagtagtcaa actatattga gcctcaaaat cttgatgaaa tactcctatt
     1801 ttggaactta tattattctt tctcttataa tccaaaacta aatttagcag taaaccacta
     1861 tcttttcctc cgctaaaaga tacatagata taatcaaatt cattaaaaag ataagaaagt
     1921 ctttcttgaa aagcttcaaa tacatcggat tctaaatatc tcttatccat ttgaattatc
     1981 ccctttcaat tagaagttta taaaacctta caatgcaata ttattatgct tttttagcag
     2041 taatcagcat aaattataca ttatttaaat acacaaatcc aactaaatac atataattat
     2101 aatgtaatta tacttttgta ttataaacta tattttatca ttattttaca tttttttcca
     2161 atatatataa ttatcatatt tttctattag ctaaaactaa ttaccttata tttttacgta
     2221 ttggtataat aatacataaa aacttgaata atgcataaaa acttgacata ctgttgtcat
     2281 aattactttg ctataattaa aagtaaaaat ggaggtaaat tctaatgtat gaacgtatgc
     2341 ttgataaaag caataaaccc tgtttgtctc aaataataga atatataggt aaagatggat
     2401 atgcacgatt gtgtaagttg gaaacttact tgaaaactca atataattta tcaagagaac
     2461 ttcgatttcc ttttggaaac aattatggtt ggggttacaa atatagccat aaatcttttc
     2521 atttatttta tgcttttttt gaaaaaggtg catttactgt tctgattcag ataggtgaca
     2581 agcaagttcc agcagtagaa aaaacgttgg tagctttgac accaaaagct aaagaactct
     2641 gggtcactcg ttatccttgt ggcaataatg gaggctggat taagtatcgt attttagaag
     2701 atgcagactt acttgatatt attcaatata taagagcaaa aaaatctcca cctaaacaaa
     2761 attaactttt attttttgtg atttaaatat agtgtatata aattccattc tttccatata
     2821 aaaaaaataa tataaaaaat atctattagt ataactcatt tagtatgtct tattctactt
     2881 tttacagata attaaaaagt tttatcataa ataaatcata actagacatc ctagtaaatc
     2941 aaaattaatt tattcttttt tattcgaatt tttgataata gtccctatac atatgccaat
     3001 tatcattcca aaaattagac cataacacat tgtaatacct acatttcctt tagaaatacc
     3061 tatagcacaa cctatagcca ttccaagtat catgcctata aacatacata taataactaa
     3121 attgttgttt ttattcatag tttttctcca ttcaatatat tttaaattgc ctagctcgcc
     3181 ttcttagctt aaaacaaaga aatataaatt aactttcttt cattctaata tctgcctgta
     3241 taaatgtcaa acaacacatt tcttgtcata acctcctctt tttgaagtaa gtacaatctc
     3301 ccagacgcaa taatatcaat ttcctaaaac catttaagta acttttatat ttacataata
     3361 tttacaatta taaactatat tgttattcat ttatacatca taaacactcc ctcctaatca
     3421 ttattatatt taaaataatg ttcatttata ataatatatc taacgcactt taattgaaaa
     3481 aactaaaact ttgcctttca ttcccatata taaacaagta tactttttga tgctctatta
     3541 caaatcaact aaattaattg atataaatta tcattttctg ttgaaaatac cattcgcaca
     3601 tgctatactt aattgcataa atggtaataa atgtcatata attttacaaa tctattaata
     3661 acaattatta cttttactgt attttaaagt ttttggacta aatatcaaga actttctaaa
     3721 atacaaagta caaaaaattt atataagtta taaatttata tgaactagat aagctcaaac
     3781 aatatgttat gtataaactg tgtagtattc ggttataaat ttatataaat tatataagct
     3841 agataaagta ttacctcaac tatataagct a
//
LOCUS       DAEORZ010000235         3848 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEORZ010000235 DAEORZ010000000
VERSION     DAEORZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.241"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..1236)
                     /locus_tag="KRH39_003680"
     CDS             complement(<1..1236)
                     /locus_tag="KRH39_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBG8513546.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIII"
     gene            1706..3157
                     /locus_tag="KRH39_003681"
     CDS             1706..3157
                     /locus_tag="KRH39_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG8513547.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKNGQAFRGGPAYYMEQGLNKKWMGVLFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLENVSEWHRELSEENLG"
     gene            complement(3379..3840)
                     /locus_tag="KRH39_003682"
     CDS             complement(3379..3840)
                     /locus_tag="KRH39_003682"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBG8513548.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSS
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
BASE COUNT         1356 a          514 c          594 g         1384 t
ORIGIN      
        1 tataataata gatataatca taagtggaaa acctattttg aaatactctt taaaagagat
       61 aggatgaccg tgtttattac ctatacctgc tagtacaaca ttagcagatg ctcctattag
      121 tgttccattt cctccaagac aagctccaag agatgttgcc caccatagtg gcattacatc
      181 aataccttca gcttgcatag tcaaaataag tgggattagt gttgcaacaa atggaatatt
      241 atctaagaaa gaagagacta tagctgatag ccataatatc aataacattg taaatacaag
      301 atgtccttct gtaagcccaa tcaatgcctg agccaatttg tttattatcc caacttctac
      361 taatccacca actactacaa ataatcccat aaagaataaa attgtagacc attcaatacc
      421 tgccataatt tcatctacat cctgttttcc tattaaaagc attatagatg ctcctgtcag
      481 tgctacaaca gaagaatcta tgtggattgt actgtgaaac ataaaaccaa aaagtatgaa
      541 tgctattaat ataagacttt ttataagtag aggtttatct ttaacagatt ttttttcatc
      601 taattttaat attgcatttt tagctctttc attcacaaca agttcttttc cataaataaa
      661 tctaaagcat attatagtta tagcaagtat tactataaca gcaggaccta aatttattac
      721 aaaatccata aaactaagat tggcagcact accaatcata atatttggag ggtctcctat
      781 aagagtagct gttcctccta tatttgaagc taaaatctgt gttattaaaa atggaactgg
      841 attaagtcct aatatttgtg taataactat agtcattggt ccaataagta agacagttgt
      901 aacattatct aatacagctg ataaaatagc tgttataata gcaaagcaaa tcattatctt
      961 ccatgggtca cctttagctt tctttgcagt ccagatagct atatattcaa ataaccccga
     1021 gtttttgaca acagctacaa atagcatcat accaactaat acaccaatag tgttgaaatc
     1081 tatatggctt aatccttcat caagtgtaag tatattgaat attatcatta atagtgcacc
     1141 accaacagct gctacggatc tgtttacctt ttctgtaata atagctgcca taactattaa
     1201 aaatatacat atagctatta tttgttgaaa ttccataata taagtacctc catttgtatt
     1261 attttgtgcc taatgctagt actatatata gtattacttt tttattgttt tttcaatgga
     1321 tgtatgaaag tatattattt aacaaagttt ttgaaaaatt gacagaattt aaagaaagtt
     1381 tttgttattt aaataaattt taaaattatt ccattgataa taatattaaa cgacaaacaa
     1441 aaaaatatac cctttaaact agaatttatt gtatccaata acaattataa aatattaaat
     1501 atatctatat tataaaaata ataaaaaaag gtttgatttg aatataaaac tgcaatgtat
     1561 taaaaaaatt attcatttta aaccttgtaa tttgatttta taaatattat aattaaatta
     1621 atgaattttt aacgaggaat tattcatttt tttgatttta agatttattg agaaaacaaa
     1681 actaaatagg ggggtaatta atataatgga ttttttcaat ggtattttaa cgagtattaa
     1741 taatgcagta gtatttgtaa acaattactt atggtcgtat atcttaatta taatgttagt
     1801 tgtaattggt atttacttta ctattaaaac aaattttgta caattcagat attttataga
     1861 aatgtttaga cttctgggtg atggtacagc gaataaaaat gcaaaaaagg aaggaaaaat
     1921 ctcttcattc caagcatttt gtataagtac atcttcaaga gttggaactg gaaatatagc
     1981 tggaatagca attgcagtgg tagcaggagg acctggtgct gtattttgga tgtggcttat
     2041 agcattaatt ggttctgcat caagctttgt tgagagtaca ttagctcaga tttacaaagt
     2101 gaaaaatggt caagctttta gaggtggacc agcgtattat atggaacaag gtctaaataa
     2161 aaaatggatg ggtgttttat tctcaatact tataactata tgttacggtt ttgtatttaa
     2221 tgcagttcaa gcaaatactg tttcacttgc atttaataat gcatttggaa taagtaaaat
     2281 ggctatggga ataggtcttg gaattttaac tgcaatagtt atttttggtg gagtgcatag
     2341 agttgcaaaa gtttctgaaa taatagttcc tgtatttgca ggattatata tattagttgc
     2401 tttggctata gttgtaatga atataacaga gataccaagt gttatagcat tgatagttga
     2461 aagtgcattt gattttaaag gaatggcgat tggtacattt atgggagttg taatgactgg
     2521 agtgaagaga ggtctatttt ctaatgaagc aggtatgggt agtgctccta atgctgctgc
     2581 aacagcacat gtaactcatc ctgtaaaaca aggtttaatt caatctttag gtgtttttac
     2641 agatactata ataatatgta gttgtacagc attcatggta ttattatatt caggttatag
     2701 tagtagtgga gcaactggaa ttgaattagc tcaagaagct ttaactcatc acataggacc
     2761 agttggaaat atatttatag cagtttgtat attcttgttt gcatttagtt ctatagttgg
     2821 taattactat tatggtgaat caaatatgga gtttatgagt ggaagtaaga ctaaacttaa
     2881 tgtatttaga gtttttgttg taggtatggt tttatttggt tcacttactc aagtagatgt
     2941 agtttggaat cttgcagact tatttatggc attgatggcg ataataaact taatagcaat
     3001 agcactttta ggaaaatatg catttattgc tttaaaagat tatacaagtc aaaaaaaatc
     3061 aggaataaaa gaccctgtgt ttattgcaga tgaaatagaa ggtctggaaa atgtatcaga
     3121 gtggcatagg gaattatctg aggaaaattt aggataaaaa aatagtataa aattacaaaa
     3181 tcattttgaa agactagagt atgtatatac tttatatgaa tataatattg aattaactta
     3241 tattactgga atttttaatt ccagtaatat ttttttatta tataatttag ttttattaaa
     3301 agtttttatt attaaaaata gcttatttat cgtaaagtaa atgatattgg cgtttttaat
     3361 tattatgaat gttggtattt aatttagaga cttaaataaa tttgccattt ctagtgcatt
     3421 catagcacaa tcataacctt tatttccagc ttttgtacct gctctttcaa tagcttgttc
     3481 aatattttct gtagttacga ttccaaatat aacaggtaat tcactgtcta aagatacttt
     3541 tgctatccct ttagagactt cgctacatac ataatcataa tgagaagtag ctcctcttat
     3601 aacacagcct aaacagatta tagcatcata cttaccactt ttagccattt tttgagcaac
     3661 aagaggaatc tcaaaagccc caggtaccca aactatttct atattttctg aagaaacgtc
     3721 gtgtcttaaa agacagtctt cagctcctga taaaagtttt gatgttataa attcattaaa
     3781 acgagaattt attataccta tttttaaatc ttttccaata agttttcctt catatatcat
     3841 tttatttc
//
LOCUS       DAEORZ010000236         3842 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEORZ010000236 DAEORZ010000000
VERSION     DAEORZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.242"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(253..1296)
                     /locus_tag="KRH39_003683"
     CDS             complement(253..1296)
                     /locus_tag="KRH39_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBG8513549.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            complement(1317..1763)
                     /locus_tag="KRH39_003684"
     CDS             complement(1317..1763)
                     /locus_tag="KRH39_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBG8513550.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            complement(1937..3517)
                     /locus_tag="KRH39_003685"
     CDS             complement(1937..3517)
                     /locus_tag="KRH39_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBG8513551.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIGMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLMGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
BASE COUNT         1232 a          712 c          345 g         1553 t
ORIGIN      
        1 aaatagacta actaaaacca tgcttatttg ctatatgttt tatataatat tatataacac
       61 tttttacatt agtaaaggat aaattgtata aagattctgt taatttatct ttataatttc
      121 atctttctaa taaaaacaaa gaagaagact taaaaattta taagtcctct tcttttcaac
      181 gtgcattttt gtgctatatt taattaattc gacctttaat ttccatatcc ttcctatgat
      241 aacaaaactc ttttattttt ttaatgattc attatcattt aaaacttctt ttatatcaag
      301 tacttctttg ttatcatcac tcataacttc ctttattcct ttgaatgttc ctaggaaatt
      361 aactgcttct gaaggtaaaa ccagttttgt tgacttacct tcagccattt tttcaagagc
      421 ttccatagat tttaatgcta gaatattatt gtctatatca gcctctttca ttgctgaaaa
      481 aactaatttt aaaccttctg ctgttgccat ttgagttctt tttatcattt cagcttcacc
      541 ttgtgcctta gcaatagctg tctgtcttat agcttcagct tcaccttctg ccactaatat
      601 agcagattct ttttcacctt ctgctacacg taccatagct tcttttttgg cttctgctgt
      661 taatatagca gattgttttt caccttcagc ttgaagtatt gcagctgact tattaccttc
      721 tgcttgaagt attgcctctc ttctttctct ttctgctctc atttgttttt ccattgcaac
      781 ttgaatatcc tgaggaggca ttatattttt taactctact ctatttactt ttatacccca
      841 tttatctgtt gcttcatcaa ggatagttct catttttaca tttattatat ctcttgatgt
      901 taatgtttca tctaaatcaa gttcaccaat tatatttctt agtgttgtag ctgttaaatt
      961 ttcaatagca gctattggat tagctatctc aaacacatat ctaactgggt ctgtaacttt
     1021 ataatataca actgtatcaa tctgcatagt tacattatct ttagttataa ctggttgagg
     1081 tggaaaatct attacaattt ctctaaggtc aattacatat gccattttat ctaaaaatgg
     1141 tattaagaaa tgtacacctg tttcagctac tttttgaaac ttaccaagtc tcattattat
     1201 acctacttta gattgtttta taactcttat acaagttaaa cttattgcta cgactactac
     1261 tattaataca atacttaaaa caattttagt acccatacta taaatcctcc tttattctat
     1321 tggtttgtca cattaactgt ggcgtctact tttttaacaa ctaattttac cccttcaatt
     1381 tttaatactt ctactgtaga acctttttct atcttttcac catctataga aatagccgtc
     1441 catgtttcat gttcaactac aactagacct gtgttatttg gagaaatctc ttctgtaaca
     1501 ataccttttt tagacatcat tgcttgtaaa ttagtgttag atttatagcc tttatctttt
     1561 tttactattt ttttagtagc tacgactaac attgctatag aaattactgc aaatattaaa
     1621 atttgtaaga aaatactctc tataaagcta cttaaaaata taagtattac agctcctaca
     1681 ctaaaccata tcaatgttaa gcttggtgtt aacatttctg ctattccaaa taagactgct
     1741 actatcaacc atattaactt catactatct accctctttt catatgaatt ataaaaccct
     1801 atataaatta tatgtttata caatgttttt gaatataaat ttaaattaca aaacaataaa
     1861 ataaattaat taattaatag tattatatca acatactttg ttaaaattgt tacaaagtag
     1921 tatcaaatat caccaattat atatccaaca actctttcat tgttaatgaa atgttactta
     1981 ttaaatccgt agcatttacc gtatacattg ctttgctcaa tttatctcct atatagccat
     2041 gtatatatgc tccactaact gtagcttcca atatatccat accttgacct ataaatgaag
     2101 taatcattcc aagcaaacaa tcacccattc caccagttgc catacagcta tttcctgtag
     2161 gatttatata tgttttttta ccatctgtta tgacagtttg ataacctttt aaaagcacca
     2221 ccagattata tttttgagca aattcttttg caacatctac tctatgttct cttatatacc
     2281 caattgaatc tcctgtcaac ctggccattt ctcccaaatg aggtgtaatt acaaatctat
     2341 ttttccattc taaaaactta taacatctgt ccttcataac gttcaatcca tctgcatcta
     2401 atactattgg gcaattagaa ttttctacta tcttaagtaa tttatcaaat gtttgactat
     2461 tatctcccat acctggacca aaaccaattg catcactaga attaagtaat ttatttaatc
     2521 tttcttcttc aaaatttaaa gtcattgctt ctgtaagtct aatagatacc ttttcttgca
     2581 catcttcact actaattaat gttacaagtc cactcccact cttaacagca gattcagttg
     2641 ctatgaaact tgcaccataa aatccttttg aacctgcaac tatagatact tttccaaaat
     2701 cacttttaaa accatgctta tctcttcttc taatattatt ttttacaaaa gtcttatcag
     2761 ttatgaactc ttcatcatgg tattttcttg taataaaatc tggaacacct atcttttcta
     2821 caactacatc acccataagt gtgctagtgt catagtttag aaaacctctt ttataaaact
     2881 caaagctaat cgttttgttt gctctaatac aagttcctaa tacatttcca ttatcgctat
     2941 ctatacctga aggaatatct atagaatatg tatttttact attatcgttt actatgtcaa
     3001 taacttcttt aaaaatacca gttatatcac gttttaatcc agtcccaaat atagcatcta
     3061 ctacaacatc acttttagat actctatctt taaaaattgt taaattttta ttttttataa
     3121 tattttgttt ctcaacttta ataattttat ctacttcatt ttcatctcca taattttttt
     3181 cacttaaatc atttttatta atatttataa tattaattcc catatttaaa agtacattgt
     3241 aatttatttt agagcattca cttagttttt caagattacc tactaaaaat acgtttactt
     3301 cttttccata tgtatttaga tgtcttgcta ttccaagacc atctcctcca ttatttccaa
     3361 caccacatac aactgtataa ctattataca tgcctatatc catatttttc attgcagata
     3421 taaccgcatt ttccatcaat acaatcattg gaatccctac ttcttctaca catgcagtat
     3481 caacatttct gcttaattta gatgtaccta ttagcataaa ttatcactcc tataaagtta
     3541 aagacttctt aaatacttac caaaaatatc tattgaaatc tttttaatcc cataaatatc
     3601 ataaataaaa tcactaaaac taagagtttt gaaataattt aaaataacaa aaaattttga
     3661 attaaaacaa ttatttaatt tattatacca tataatttaa gttattatgt tgatttaaaa
     3721 cctatttaca ctaattatct aaatatctct ttatttttat aatcttttat gacttattta
     3781 tatttttcat ttttataaaa aaacaataat taagaatata actatattct acatgtaaaa
     3841 at
//
LOCUS       DAEORZ010000237         3778 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEORZ010000237 DAEORZ010000000
VERSION     DAEORZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.243"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            93..1145
                     /gene="ytvI"
                     /locus_tag="KRH39_003686"
     CDS             93..1145
                     /gene="ytvI"
                     /locus_tag="KRH39_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG8513552.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFHINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            1235..2461
                     /gene="pepT"
                     /locus_tag="KRH39_003687"
     CDS             1235..2461
                     /gene="pepT"
                     /locus_tag="KRH39_003687"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBG8513553.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPKIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            2668..3720
                     /locus_tag="KRH39_003688"
     CDS             2668..3720
                     /locus_tag="KRH39_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBG8513554.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT         1461 a          395 c          697 g         1225 t
ORIGIN      
        1 atttatacac ttctttttat tttgtattca aataaaacta gtatgaaatg gacaaaataa
       61 aaatataaat tagaagaaag gagattttta agatgagcat actagaaaat aattttttat
      121 acaaattaaa aaacaacact atcttttttt gtatatatac attgttattt atttttattt
      181 acaaagcgtt tccatacata gctccatttt ttctaggtgc tataatagca cttatgatta
      241 atcctatatc acaaaagtta gaaaataagt ttcatataaa taaaggtatc tctactcttg
      301 ttctaagttt tcttgcagtg gctatagtca gtacagttac tacaataata gtaataaatt
      361 caatgaaaga attaatggga tttttaaata atatatctgc taatcctgag gatataagta
      421 atactattat gtatttttta aataaaataa atgactttat gaaatcattt caagaaatag
      481 ccaattttga tgtagaacaa ttagtaaaca aattcagtgg tgaagtaatg caaattacaa
      541 aaaatcttct tactagtata cttggattgg ctacttctat tccatatatt ataatattta
      601 taattacctt gtttattgct acgtatttta tagcaaaaga ccttgataag attgaaaata
      661 gtttttataa tatgtttact gttgatgtta gaaagaaggt aaaaaacgta aagaaagagg
      721 cagggctttc acttgttgga tatataaggg cctatacaat acttatggct ataacatttt
      781 ttgcaatctg gggaagtttt gctttatttg gattgaaata cggtctaata gtagggtttg
      841 taggagcttt aatggattta attccattcc taggtataat aacaatatat cttccagtaa
      901 ttgtttatta ttttcttata aaaaattatt ttatagctat aagtatgact gtgatatttt
      961 ttgttttgtc tttgattaga gaaatacttg aacctaaact agtatcagta aatgttggac
     1021 taaatccatt agctacccta gcagcgattt ttattggaat ccaagtaaaa ggaataattg
     1081 gagtaatttt ttgtttgggt ttggtatgta tgcatgatat acttaagaag gtggatattt
     1141 tttaattata ataattgata gattttttaa aaataaaatt ttattcacag gtaaataaaa
     1201 aaatattaat cttattaaga aagagggtat atacgtgaaa caaaaagtag tggaaagatt
     1261 tttaaaatat gtatcatttg atacaacatc taattctcag tgtgagaact gtccatcttc
     1321 agaaggtcaa agagttttag ctaagtatat agttgaagag ctaaaaacaa tgggtgttga
     1381 tgatgtgagt ttagatgaaa atagttatat aatggctact ttaaaaggaa atactgatgg
     1441 tgtggatact ataggattca tatcacattt agatacaatt gaagatgtta gtggaaaaga
     1501 tattaaacca aaaataatag aaaattatga cggaaaagac atagttttaa atgaagcgtt
     1561 aaatgttatt acgtatgtaa aggattctcc agagttggaa gaatttaaag gtgacgattt
     1621 aatagtcact gatggaacaa ctctacttgg gtctgatgat aaagctggta tagctgagat
     1681 agtgactgca atagagtatc taataaatca tccagaaatt aaacatggag atataaaaat
     1741 aggatttaca ccagatgaag aaattggtag aggtgctgat ttatttgatg ttgaaaaatt
     1801 tggagcaaaa tatgcatata ctttagatgg aggaatagtt ggagaacttg aatgtgaaaa
     1861 ttttaatgct gcaaatgcaa ctattactat acatggtaga aatgttcatc caggttcagc
     1921 taaaaataaa atggtaaatg ctatacatat agctgctgaa atatcagaaa tgttcccagc
     1981 agatgaaaga ccagaaacaa cagaaggata tgaaggattt tggcatttaa attctatagg
     2041 tggaaatgtc gagaatgtaa gtatggctta tattattaga gaccattgta aagaaaaatt
     2101 tgaaaataga aaatctatta tgatagagaa tatagaaaaa ataaataaga aatacgataa
     2161 tagagtggaa ttggatttaa aagactctta ttacaatatg aaagaaaaga ttgagccagt
     2221 tatgtttata gttgatatag ctaaagaagc tatggaggaa ttaggtataa aaccacgttt
     2281 agttcctgta agaggtggta ctgatggagc aagattatct tttaatggat taccttgtcc
     2341 aaatatattt actggtggat taaacttcca tgggaaaaat gagtgtatac cagttagttc
     2401 tatggagaaa gctactaaac taattgtaag aatagctgaa aaatatgctg aaagagttta
     2461 gttaaaaagt atttaaaaat taagcattta aagttataat agctatatca ttctttataa
     2521 gtcaagctga ttataagctt gtgttgtact taaaatggta tagctatttt tgtaattatt
     2581 tttataaaag ataattattt ttagtttagg gtatcaataa aaagtatgaa tatcaatagg
     2641 tattaacatt taaaaggggg aattgtattg aaaattaaat ctaaaaaaat aatagcattt
     2701 gcactttcac ttgggattat gttgccaagt tgtgtatatg cagacactat tagtaaaaat
     2761 caaaatgcac aggtagatat gaacaattat cttcaaggta gaaaatctcc aaaggaggaa
     2821 aaacttattg gtgaagatag atttgataca gcgataaaaa tatctcaaag tggttggaat
     2881 aatggttcag aaagggtgtt tctagttaac tcaaattcat tgccagatgc attggcttca
     2941 actcctttgg caagtaaatt ggatgcacca atactgctta caaataaaaa tagtatacca
     3001 tataatgtaa cagaggaaat aaaaaggctt aatccagcag aagttatttt aattggaagt
     3061 gaaggagcta tttcatcaag tgtaaaagaa tctcttgaag atatggaagt attagtcagt
     3121 agaatcggtg gagcagatag agaagaaaca tctttacttc ttactagaca gctagatgat
     3181 actggtgatt taggtgtctc aaaagtggca gtggttaatg ggtataatgg actggcagat
     3241 gcaacttcaa tatcaagccc agctgcaagt gataatacag ctataatata tacaggtaag
     3301 gatagcataa gaagtgaggc aaagaatttt attacacaaa actcaactaa gacatacata
     3361 gtaggtgggg agtacagtat aagtaaaaaa cttgaaggac agttagtaaa ctcagaaaga
     3421 ctagcaggga cagatagaaa agatactaat gcgaaagttt tagaaaagtt ttatggaaaa
     3481 tcaagtaagg ttaataatat gtattttgca aaagatggaa gtggtagaga agctgattta
     3541 gttgatgggc ttgctgctgg agtattagca tcaaaaacaa agtcacctgt agtacttgca
     3601 agtggaagtt taagtagtgc acagaaaagt tttataaaaa aagtaaaggc tgataaattt
     3661 gtacaagttg gtggaggtaa aaatagtaaa ccatatgcag aagcattagc cttacagtaa
     3721 tattgtgtaa atcaaaatta atatataata atagcaagta aaccaagttg cactttaa
//
LOCUS       DAEORZ010000238         3775 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEORZ010000238 DAEORZ010000000
VERSION     DAEORZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.244"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            251..400
                     /gene="rpmG"
                     /locus_tag="KRH39_003689"
     CDS             251..400
                     /gene="rpmG"
                     /locus_tag="KRH39_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBG8513555.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            426..647
                     /gene="secE"
                     /locus_tag="KRH39_003690"
     CDS             426..647
                     /gene="secE"
                     /locus_tag="KRH39_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBG8513556.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            667..1209
                     /gene="nusG"
                     /locus_tag="KRH39_003691"
     CDS             667..1209
                     /gene="nusG"
                     /locus_tag="KRH39_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBG8513557.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1248..1673
                     /gene="rplK"
                     /locus_tag="KRH39_003692"
     CDS             1248..1673
                     /gene="rplK"
                     /locus_tag="KRH39_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBG8513558.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1744..2442
                     /gene="rplA"
                     /locus_tag="KRH39_003693"
     CDS             1744..2442
                     /gene="rplA"
                     /locus_tag="KRH39_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBG8513559.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2494..2622
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2665..3171
                     /locus_tag="KRH39_003694"
     CDS             2665..3171
                     /locus_tag="KRH39_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBG8513560.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3229..3594
                     /gene="rplL"
                     /locus_tag="KRH39_003695"
     CDS             3229..3594
                     /gene="rplL"
                     /locus_tag="KRH39_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBG8513561.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
BASE COUNT         1525 a          453 c          747 g         1050 t
ORIGIN      
        1 attctttact aaactgatgg attaaaagga ataacaaaaa gaggggttat gctcctcttt
       61 ttttaacata gaaatacaaa aacattgaca cagcatagga ttttgtgata acttatacaa
      121 ggtaatgatt ataggaagtt aataatatct tgaatactgt agatttttaa taaatacata
      181 ttttaaagat attatattca catatattaa caatatatat tattgtatta caaagttgga
      241 ggtgtatcag atgagagtta aagtaacttt agcatgtaca gagtgtaagc aaagaaacta
      301 caacactact aagaataaga aaaacaatcc agacagaata gaattacaaa aatattgtag
      361 attctgtaaa aagcatacaa ctcataaaga aacaaaatag ttttttataa ggatgtgagt
      421 atggaatggc tgcccaaaaa aatgacggtg caaaaactaa aaaaaggttt agtttgtttg
      481 gatacttaaa agaaactaaa caagaattaa aaagagttac atggccgaca aaaaaggaac
      541 tgtttaaaaa cacaagtata gttttaactg tagttatttc atgcactata ttagtatggg
      601 gtatagacac tatattatct ggtgcactag cgttattact aaaatagacg aaaagaggta
      661 agttaaatgt cagaattaca agaagcaagt tggtatgtag ttcataccta ttcgggacat
      721 gaaaataaag ttaaagctac aattgagaaa gcagttaaaa caagaggtat ggaagactgc
      781 ataagacaag tagttgttcc aacagaagaa gtagtggaaa ctactaaaac tggaaaagaa
      841 aaaactagac aacgtaaagt ttatccaagt tatgttttgg ttaaaatgat aataactgat
      901 gaatcttggt atgtagtaag aaatactaag ggagttacag gtttcgttgg acctggttca
      961 aaaccagtgc cattaagtga agacgaagtc aaagctatgg ggatagacac aacagaccct
     1021 aaagtagtaa atagcgatat tgattttgaa ataggagata cagtaaaagt atctcaagga
     1081 ccatttagtg gacaaatagg taacatagaa gaaattgact tagaaaatag agaagtcaaa
     1141 gtatgcataa atgcatttgg taaaaggact ctatttgtaa tagagcttga aggtatagaa
     1201 aaaatctaat actatattat agaaaaatct gaggaggtgc gctcaaaatg gctaaaaaag
     1261 ttataggtca aataaaatta caaatacctg caggaaaggc tactccagcg ccaccagttg
     1321 gaccagcatt aggacaacat ggtgttaata taatgggatt cacaaaagaa tttaatgcta
     1381 aaactgcaga tcaagctgga atgataatac cagttgttat aactgtatat caagatagat
     1441 cattcagttt tataacaaaa actccaccag ctgcagtttt aattaaaaaa gcattaaact
     1501 taaaatcagg ttctggagaa ccaaacaaga aaaaagttgc taaaatgact tcagctcaag
     1561 taagagaaat agctgaatta aagatgcctg acttaaatgc tgcatcagta gaagctgcta
     1621 tgagtatgat agctggtact gctagaagca tgggtgttgt aatcgaagac taattaaaag
     1681 atacactttt ttttataaag tgggaggcga gaagccgaaa ttaaccacaa ggaggaaaat
     1741 aaaatggcta aaaaaggtaa aagatatgca ggtgcattac aaaaagtaga tagaactaaa
     1801 ttttatgatg catcagaagc gttaacatta gtttcagata tagctggagc taaatttgat
     1861 gagacagtag aagcacacat taaattaggt gttgactcaa gacatgctga ccaacaagta
     1921 agaggtgcag ttgtattacc tcatggaact ggtaaaacaa aaagagtttt agtttttgct
     1981 aaaggtgaaa aagctaaaga agctgaacaa gctggtgctg actttgtagg agctgaagaa
     2041 ttagttcaaa aaatacaagg tgaaaactgg tttgactttg atatagtagt tgctactcca
     2101 gacatgatgg gtgtagtagg tagattaggt agagtattag gacctaaagg tttaatgcca
     2161 aaccctaaat caggaacagt tacatttgat gtagctaaag ctatagatga aataaaagct
     2221 ggtaaagttg aatacagatt agacaaaact aacataatac acgttccagt aggaaaagta
     2281 tcatttggtg gagaaaaatt aactgaaaac tttactgcat taatggatgc tataataaaa
     2341 gctaaaccag ctgctgctaa aggacaatat ttaagaagta taacagtagc ttcttcaatg
     2401 ggacctggag ttaaaataaa cccagcaaga acagctgaat aataaaactg ttgacacagg
     2461 ttgacaaaaa tgttattata tcacttgtca atgtaaaaaa gaatagattt gccgtagata
     2521 gtgggtgcga aagcttaata tcccgccgag gttttagata aattgagatt actaaagctt
     2581 ttgatgtcta cttttggcaa caaaagcttt ttataatgtt gcggtaaata ctaagcctat
     2641 ttggtgaagg aggtgcacat ctatatgaga aaagctatag aaattaaatc agaagttgtt
     2701 tctgagatag ttgaaaagtt acaaaaatct tctgctgctg ttgttgtaga ttacaaagga
     2761 ttaacagttg aagaagtaac tgagttaaga aaacaaatga gagaagctgg tgtagattac
     2821 aaagtataca aaaatacttt agttagaaga gcagctaaag aagttggtat agagcaattt
     2881 aatgatgaat tattagttgg aactaatgcc atagcatttg gatatgatga tccagttgct
     2941 ccagctagaa tattaaaagg attcatggat tctcatccaa aaatgaaatt aaagatgggt
     3001 attgtggaag gtgcattcta tgatgagtct aagattgttg aaatggctaa cataccatca
     3061 agagaagttc ttatcgctaa attacttggt agcttaaaag ctccagtatc aaacttcgca
     3121 tacttaatag atgcaatagc gaaaaaggca gaaggtcaag aagaagctta attgcttaac
     3181 taaaaaatta taaatattaa tttgaaataa aattcggagg tgctaaaaat gacaatagaa
     3241 caaatattag aagctataga aaatatgaaa gttttagaat taaatgaatt agttaaagct
     3301 gctgaagaaa aattcggtgt atcagcttca gctccagtaa tggtagctgg tgcagctgct
     3361 ggtggaccag ctgctgaaga aaaaactgag tttgatgtag tattaactga cgttggttct
     3421 tcaaaagttg gagttataaa agcagttaga gaaataacag gattaggatt aaaagaagct
     3481 aaagaagtag ttgacaatgc tcctaagaca gttaaagaag gagcttctaa agaagaagct
     3541 gatcaaataa aagaaaaatt agaagctgct ggagctaaag tagaagttaa gtagtttctt
     3601 taaaaagata cccattgggt atcttttttt atttataaaa aattataaag aaagtatttt
     3661 tataaaataa aaaaaattta tataatagta ctaaattaag ggtatcatat atggtgtaat
     3721 ggtattttaa tttaacatta ttatcaaatt aaaatataaa caattgttgt aatgt
//
LOCUS       DAEORZ010000239         3756 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEORZ010000239 DAEORZ010000000
VERSION     DAEORZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.245"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(62..349)
                     /locus_tag="KRH39_003696"
     CDS             complement(62..349)
                     /locus_tag="KRH39_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513562.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(369..644)
                     /locus_tag="KRH39_003697"
     CDS             complement(369..644)
                     /locus_tag="KRH39_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513563.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(1278..1457)
                     /locus_tag="KRH39_003698"
     CDS             complement(1278..1457)
                     /locus_tag="KRH39_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513564.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(1742..3217)
                     /locus_tag="KRH39_003699"
     CDS             complement(1742..3217)
                     /locus_tag="KRH39_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8513565.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYITLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
BASE COUNT         1328 a          602 c          365 g         1461 t
ORIGIN      
        1 cctttagttt aaataattca cctataccta cttgaatagt tcacttttat ttgcttaaat
       61 tctataccaa tactggctta aacattatac taccatgatt gtattgtcta tcttcaacta
      121 caataaaaac aggttctaaa tctttagtag atgttatttt taattctaaa aaaacattat
      181 cagttggtaa tatttttaca tcataattat ttaaattatt attagtgata ctattaaata
      241 catcaccttg acgttcaaaa aaaacattat caactattac aaaataaaca tcttcttttc
      301 tactattttc aacctctata gtattttgtt tagttacatt tagtctcatc ttccacctcc
      361 tattaatatt acttaattat attaatactt gtaattgttg cattatgttc atcacaagga
      421 agaggtttaa aaacaactat ctctgctaaa taattctgtg gaggatttgt aactgaagta
      481 gcattgcaaa taaatttaaa atttgttcct gctacaactt gagtagaaac aataagtggt
      541 tcataaccaa ccccttctaa cattcctaca gcttcattaa atacttttaa atcttcttct
      601 tcgacagctc tgaattttga ccatccacca acaaaaagac tcatataaaa acctccttta
      661 tatatatatt tatttatata tagtagtacc aattttctca atattcaata ttttctgtgt
      721 aactggtcaa tgaaaatgca taactggata aaaatactta taaaataact taactttaca
      781 tataaatagc ttactttaca tgtaataaga ttattctaca atatgaaata catcttgttt
      841 tgaatatatt ctaaatacaa taatggatat tttcaattat tatccttaga aaatatccat
      901 tacttaactt gttttattta aaatcacata gagactttat tatttttttt attgcatgaa
      961 tagctatttc aggtatcata aggtgatata ccccctcctt gaaaccacaa aaaacatgac
     1021 aaactttacg aatcttggct taaaaaacta caggtacatt ccttatttgt ggattggagt
     1081 agccataaca agcaaatttt acaattttta tgatgacaaa atcaataaaa tcgcttagta
     1141 tataacacga aacactactt gttgctatta agaatagtta gataggaagt atgcattata
     1201 ccaataaagg aatcaataaa gaaagttaaa acatccctac aaatcaaaaa tctgttacat
     1261 ttttctatat ttaaacgtta tttacttaca attggagaaa taacaggttt tccttctcgg
     1321 tcaagtaaag gcgtaaatcc ggctgcattt ccataatagt ggaatacata gttcacacct
     1381 gtttctttat ccacccaaat ctccgttgca tttatttttc cttgtgaata tactttttca
     1441 aatctatctt ttcccatttt ataatccccc atatttttga ttggtctata ccttcttcac
     1501 ttaagtcgaa aaaatacata acttattttt ttaattctaa tatgaacacg taaaaatgtc
     1561 aaacagctta tttaatttca taacttcatg ttactgttcg taaccttgtt tgtttactta
     1621 cactacatta cagcaccatt ttacccttta catgattgta ttgcacacag caaaaatgct
     1681 tactttattg gatttttcaa ctaaaaaaag gtttttttct ccacagaaca tttactattt
     1741 tttataatat atctaatttt actctcatat caacatttaa tgaagatata acctttttca
     1801 aatattgtaa agactcctta aaaatattag aaaaagattc tgcaaatctc tcaacatctc
     1861 ctttttttaa atacctaagt atctcaaaac atacatcaaa acgtgttttc aaaatttcat
     1921 tatattcttc atgaaaaaat ctctctaaat agattccaaa tatcatatat tgttttaaat
     1981 gcaaaaatgt ttcctgtaat acataaattt gtgtataatc tactatctta caaagtaaaa
     2041 caaaatctgg ataaacctta cactcttcac aattattttg taagtacctt aaatgagttt
     2101 ctattttatc tacagctatc aaatccattt tatttcctag taaaagtact acatcctcaa
     2161 gagtaatcgt aacaaactcc atagcatcca aaagcaaact caacctttta gctccttctt
     2221 ctgtatttat ataatcatca tcatattcag aaaaaagtgt tatatatgtt cccttgccat
     2281 taattgtttt agcaattccc aattcattta gctcattata ggcacttcta actgttggaa
     2341 gagatacatt atatctatta attatcgaag taattgatgg tagacaatct cctattttaa
     2401 tattgccttc aaaaatttga caaattacat ctgaaacaat taagtcatat aaataacatt
     2461 tttccaacgt tacagaatta aaactacttg tatctatact ttgtggagca cgattttcta
     2521 ccattttaag tatggattct ctacaagctt tatacatata tgttaatagt tttttaaatt
     2581 caattagatt cccatccaat ccatataagt aagtttttct tacctgctct atgtatttat
     2641 tacgaaaaaa gtctatacaa caattatcca agttatccaa gttatcgaaa ttataggcca
     2701 attgtgaaat aatgacatca tgctgaataa tctcacaaaa atggtgaatc aggaaattat
     2761 ttgttttact tattagttta tttatatatt ttagcctttg ttctttaaag actaaataat
     2821 tagaactatt tttttccata caatcgataa tctcgtttaa ttcttcaaag tctttagatg
     2881 taaatctttt aatattgtac atagcaatag aaggaaatat taaactaaaa atcttatata
     2941 tttctgatat ttgtaaaatc ttctcatata ataagtcaga ttctattgat tgtttactcg
     3001 tactctggat taaaactggt ctagaaccag cttttgtctc tataaagccc ttttctgaga
     3061 gcatttttat tacagttttt atagtatttt ttgataccat atattcttct gacatgcttt
     3121 ttatagaatc aagcctatct ccaatagtaa ttttaccagt ataaatttgt tgaattatat
     3181 gctcaaaaat aattaaatat tgtggttttc tcttcatatt tttcctcttc gataaattag
     3241 atttattgtt ttttgtaagg tatatttact tttatttaat agggcaaaat agttgcttat
     3301 tttttcctca taatctataa ttaatataaa ccatttatat atatggtcta agttttataa
     3361 tactgtaatg cagtaaaata gcatattgta gctatactac agcaaattaa cataccattg
     3421 tagtatatta attggaataa ttttaagtac ttcaaaattc cagcaaaaca tatatgtgtt
     3481 tttgatatta tgtatattcg ttaatagaat agctatactt gttttacata ataaattata
     3541 tgatacttgc tatatgtaat tactatattt aatacacata actattatac ttactatatg
     3601 taattactat atttgataca cataattatt atacttacta tatataatta ctatacttgg
     3661 acacataact attatacttg ctatatgtaa ttactatact ttatacacat agttatcata
     3721 catgctatat ataattacta cacttggaca cataac
//
LOCUS       DAEORZ010000240         3755 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEORZ010000240 DAEORZ010000000
VERSION     DAEORZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.246"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            84..1952
                     /locus_tag="KRH39_003700"
     CDS             84..1952
                     /locus_tag="KRH39_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513566.1"
                     /translation="MEKSSIKILIGLFIIGVIIILIFLNRTKTKNYLYQIFNVIINST
                     KLIFNYSWIYIFLCIIFSVFQGFFPIISLLIMQKMINAIQMKNIGQDIFLKLIFIYIV
                     LEMIDLMAVEIYNLYSTTFMKRFTRHIEILMLEKANRLTTKDFENKEIYDLINRAQSQ
                     NGATVIDYFNSWILIFQQAITIFSSIIIIISYQYWIVILIIIAPVIQFYYSQKLGHEQ
                     YEISMKRTSEERKCWYINFLMLLGNSIKETILYGLGNLFINQYGKLKEKFILQDYKIM
                     KKTFEMNFIIIIIGQLFTGVIYFHILLNGFWQKIYMGDVTTYITCITTIKNGIEVILS
                     MMSTIYSKSLYMELLFRFFDIPEKTELNKTKLKIDNIEKIELKHLYYKFTEDSGYVLK
                     DINLELKKNSPIALVGRNGSGKSTLIKIILGIYDDYEGDILINGINLHNIEKENYYNK
                     ISCVFQDYTKYEASVKENVGYGNLDQINNEDLIKESLIKSGLDINQLGSNKLNTILGY
                     WFGENQLSEGQWQKIAISRAMMKDADFYILDEPDAALDGIAENDMLLTYKKMFENKFG
                     IFVSHKTHHLNLLCENIIVLNNGQIAECGNHEELIEKEGIYHQLYSSQIISKLETN"
     gene            1981..3408
                     /gene="ccpM"
                     /locus_tag="KRH39_003701"
     CDS             1981..3408
                     /gene="ccpM"
                     /locus_tag="KRH39_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HBG8513567.1"
                     /translation="MEQKPFIHLFQTSTGFYFYDVNTDSILNIDENVYDYLKKEQLGE
                     SSSNTLEYVEFLRKQGYLKSNKVKITEHPETEVLKYHCENRVEGIILQVTQNCNLHCD
                     YCTYSGGYINRIHTNKRMSKETAKRGIDYLISHSRDCQYVSIGFYGGEPLLEFDLIKW
                     CIEYSKANIEGKNLKYNLTTNATLITEEIIELFEENNVSIMISLDGPAEIHDKNRKFA
                     NNNVGSFSTIMKKLDMISTKYPNYFKNNVHFNSVLATDNFSCVNKFFTKEELFQNSLF
                     LSSIVSEVNAKVKTEKSQSFYEESQYALFIGYLTLLGRIKKEDSSKLLQTQISTIGDT
                     RNGKQGKQRLVMPEKWHHGGPCVPGVMRLFINVDGYFYPCEKASEASDSMNIGNLDQG
                     YDLKKVEKLLNIERINGDKCHSCWAYENCRICAVSLSVDSTQEDIAKECEYIRSSIES
                     DFQDYCVLKELGFDFETKELIENTI"
     gene            3471..>3755
                     /locus_tag="KRH39_003702"
     CDS             3471..>3755
                     /locus_tag="KRH39_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBG8513568.1"
                     /translation="MKKVLIYPFTEEMAPFVKQENLLKDIDITSIVSLKGNGILGDKY
                     KFNGKSLEVSENFDKELEKCTYILLNNFYSDESENIILENIKKAVNHGKKI"
BASE COUNT         1563 a          348 c          576 g         1268 t
ORIGIN      
        1 agcaataaat tgcttgataa tatattttta tgtgaaaaaa acatcaaaaa aatactttta
       61 agaatttaat ttcaggtgaa ataatggaga aatcatcaat taaaatatta attggtttat
      121 ttattatagg agtaattatt attttaatct tccttaatag aactaaaact aaaaattatc
      181 tttatcaaat ttttaatgtt ataataaaca gtactaaatt aatctttaat tatagctgga
      241 tatatatttt tttatgcatt attttttcag tatttcaagg tttttttcca ataatatctt
      301 tgttaataat gcaaaaaatg ataaatgcaa ttcagatgaa aaatataggg caagatatct
      361 ttttaaaatt aatttttatc tatatagtct tagagatgat tgatttaatg gctgtagaaa
      421 tttataattt atactctacg acatttatga aaaggtttac cagacatata gagattttaa
      481 tgttagaaaa agctaataga ttaactacga aggattttga aaataaggaa atttatgatt
      541 taatcaatag agctcaatct caaaatggag ctacagttat tgattacttt aactcctgga
      601 ttttaatttt tcaacaggca atcacaatat tttcctcgat tattataatt atatcttatc
      661 aatattggat tgtaatatta atcataatag ccccagttat acaattttat tactctcaaa
      721 aattagggca tgagcagtat gaaataagta tgaaaagaac ttctgaagaa agaaaatgtt
      781 ggtatattaa ttttttaatg ctattaggaa attcaatcaa agaaacaatt ttatatggat
      841 taggaaattt atttataaat cagtatggaa aattaaaaga aaaattcatt cttcaagatt
      901 ataaaataat gaaaaaaaca tttgagatga attttataat tataattatt ggtcaattat
      961 ttacaggagt tatatatttt catatacttc ttaatgggtt ttggcagaaa atatatatgg
     1021 gagatgtaac tacttacata acctgtataa caacaataaa aaatggtata gaagtgatat
     1081 tatcaatgat gagtacaata tatagtaaat ctctatatat ggaactatta tttagatttt
     1141 ttgatattcc tgagaagacg gaacttaata aaacaaaact taaaatagat aatatagaaa
     1201 aaatagagct aaaacattta tattataaat ttactgaaga ttcaggttat gttctaaagg
     1261 atattaatct agaattaaaa aagaatagtc ctattgcact tgttggtaga aacggttctg
     1321 gtaaatcaac tttaatcaaa attatactag gaatttatga tgattatgaa ggagatatct
     1381 taataaatgg aattaaccta cataatatcg aaaaagaaaa ttactacaat aaaataagtt
     1441 gtgtttttca agactatacc aaatatgaag ctagtgtaaa agaaaatgta ggatatggaa
     1501 acctagatca gataaataat gaggatttga taaaagagtc attgataaaa tctggactag
     1561 atattaatca attggggagt aataaattaa ataccatttt aggatattgg tttggagaaa
     1621 atcaactttc agaaggccaa tggcagaaaa tagcaatatc aagagctatg atgaaagatg
     1681 ctgattttta cattttagat gaaccagatg ccgctttaga tggaatagct gaaaatgata
     1741 tgcttttaac ttataaaaaa atgtttgaaa ataaatttgg tatattcgta tctcataaaa
     1801 cacatcattt aaatttgtta tgtgaaaata taatagtttt aaataatgga caaattgcag
     1861 aatgtggaaa tcatgaggaa ttaatcgaaa aagaaggaat ttatcatcag ttatattcaa
     1921 gtcaaattat aagtaaacta gaaacaaatt aataattact ttgaaaagga gttaagaatt
     1981 atggaacaaa aaccttttat tcatttattt caaacatcaa ctgggttcta tttttatgat
     2041 gtgaatactg attctatatt aaatatagat gaaaatgtct atgattatct aaagaaagaa
     2101 caacttggag agtcaagtag taatacatta gaatatgtag aatttttaag aaagcaagga
     2161 tacttaaaat caaataaagt gaaaataaca gaacatccag aaactgaagt attaaagtat
     2221 cattgtgaaa atagggtaga aggaataata ttacaagtaa ctcaaaattg taacttacat
     2281 tgtgattatt gtacttattc tggaggatat ataaatagaa tacatacaaa caaaagaatg
     2341 tctaaagaga ctgctaaaag aggaattgat tatttaataa gccattcaag agattgtcaa
     2401 tatgtgagta taggttttta tggcggagag ccattactag aatttgattt aattaaatgg
     2461 tgtattgaat attcaaaagc gaatatagaa ggtaaaaatt tgaaatacaa tttaacaact
     2521 aatgcaactc ttataacaga agaaattata gagttatttg aagaaaataa tgttagtatt
     2581 atgattagtc tagatggtcc tgctgaaata catgataaaa atagaaagtt tgcaaataat
     2641 aatgtgggat cttttagtac tataatgaaa aaactagata tgatatctac taagtatcca
     2701 aattatttta aaaataatgt acattttaat tctgtattag caactgataa cttctcttgc
     2761 gttaataaat tctttactaa agaagaatta tttcaaaatt cattattttt atcttcaatt
     2821 gttagtgaag taaatgctaa agttaaaact gagaaatcac aaagttttta tgaagaaagt
     2881 cagtatgctt tattcatagg atatttaact ttacttggta gaataaaaaa agaggatagt
     2941 tctaaattat tacaaactca aatctctact attggagata cgcgaaatgg taaacagggc
     3001 aaacaacgtt tagtaatgcc agaaaaatgg catcatgggg gtccatgtgt accaggagtt
     3061 atgagattat tcataaatgt tgatggttat ttttatccat gtgaaaaagc atcagaagca
     3121 tcagatagta tgaatatagg aaatttagat caaggatatg accttaaaaa agtagaaaaa
     3181 ttattgaata ttgaaagaat aaatggtgat aaatgtcata gttgttgggc ttatgaaaac
     3241 tgcagaattt gtgctgtttc tttaagtgtt gattcaacac aagaagatat agcaaaggag
     3301 tgtgaatata ttagaagtag tatagaaagt gatttccaag attattgcgt attaaaggaa
     3361 ttgggatttg attttgaaac aaaagaactt atagaaaata ccatttaatt taagatagca
     3421 tcttaaatta ttacattatg aaactaatta gaatctattg gaggattaaa atgaaaaaag
     3481 tattaattta tccattcaca gaggaaatgg ctccatttgt taaacaagag aatttactaa
     3541 aagatataga tataacatca attgtaagtt taaaggggaa tggaatctta ggagataaat
     3601 ataaatttaa tggtaagtct ttagaagttt ctgaaaattt tgataaggaa ttagaaaaat
     3661 gtacatatat cttacttaat aatttttata gtgatgaaag cgaaaatatt attttagaaa
     3721 atataaaaaa agctgtaaat catggtaaaa aaata
//
LOCUS       DAEORZ010000241         3734 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEORZ010000241 DAEORZ010000000
VERSION     DAEORZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.247"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            227..1846
                     /locus_tag="KRH39_003703"
     CDS             227..1846
                     /locus_tag="KRH39_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG8513569.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDEHFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2220..2414
                     /locus_tag="KRH39_003704"
     CDS             2220..2414
                     /locus_tag="KRH39_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8513570.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(2973..3296)
                     /locus_tag="KRH39_003705"
     CDS             complement(2973..3296)
                     /locus_tag="KRH39_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513571.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(3341..3595)
                     /locus_tag="KRH39_003706"
     CDS             complement(3341..3595)
                     /locus_tag="KRH39_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513572.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
BASE COUNT         1371 a          479 c          457 g         1427 t
ORIGIN      
        1 aaatactaaa aatctaagtt caagtattta aattgctata tatcataaat tttctaaaat
       61 taacaacaac ttaaaaattt aacattctct taacgtatac ttaacaaaac attaatattt
      121 ttattgacaa aatttgttat tctatattat ggaaacaata aattttagtt aataaagttt
      181 ataattcaga taaaaaaatt attaatttct taagggagga aatatcgtga atatagtaat
      241 tagccttatc ggtggacttg gactttttct ttacggaatg agtttaatgg gagaaggttt
      301 acaaaaatca gcaggagaca aattaaaaaa aattattgaa ctcttaacaa gtaatgttgt
      361 tatgggtgta ctggtaggta cagtcgttac aggtattata caaagttcaa gtgctactac
      421 tgttatggtt gtaggatttg taaatgcagg tataatgaat ctaagtcaag ctattggtgt
      481 aataatgggg gcaaatatag gtactacagt tactgctcaa ttagtttcat ttaatcttga
      541 aggaattgca ccaattgctt taggtatagg tattttattc tatttattta catcaaatca
      601 aaaaacaaag catttatctg agatacttat aggttttggt atattattta caggaatgga
      661 gtttatgaaa gatgcagtag ctccacttgc tgaatacaaa gcattcactg atgcactttt
      721 atattttagt aaaaatcctg tattaggtat attggcagga tttgccataa ctggtattgt
      781 tcaaagttca agtgcatcta tgggtatgtt aatagcacta gcttctcaag gaatacttcc
      841 tctatcatct gctttaccta ttctttatgg agataatata ggtacttgtg ttacttcact
      901 tttatcaagt gttggtgcaa gtagaaatgc tcgacgtgca gctgtaatgc atttaagttt
      961 taatgtaatt ggtacaataa tatttatgct tgttttaaat aaacctatat cagccatagt
     1021 tactcatttt gacccaactg atacagctag acaaatagca aatgcacata ctttatttaa
     1081 tcttacaaat gttataatat tattaccatt ttctaaatac atagttaaat tagcaaatag
     1141 acttatacct attaaagaaa ctgaatcaga aatagtaaat aatactaagt acttagatga
     1201 aaggatgttt agcactccat ctatagcact tggtaatact gtacaagaag ttgtaagaat
     1261 gggtcataaa gctacaaact ctttggaaca ttcaattgca ggatttttaa ataagagcaa
     1321 tgaagatatt aataaagctt ttgagtcaga aaaagttgtc aataagttac agaaagatat
     1381 attgaactat ttgttaaaac tttctaaaga acctcttaga gatgatgaac actttagaac
     1441 agatttgctt tttaatactg taaatgacat tgaaagagtc tctgaccatg cagaaaatat
     1501 agctgaattg gccatgagtg ttaaggaaat gaacattagt ttttctgata gtgcgataag
     1561 agaaatatac gaaatataca ataagacaat tactaatttt aaggatgcct taatagtttt
     1621 agatgttaaa gattttgagt tagcaaataa agttttagaa gttgaaaatg aagtaaatta
     1681 tttagaaaaa acttttagaa attcacatat gattagacta aataatggaa gttgtacaat
     1741 tgatgctggt gtactttatc ttgacttact tactaatcta gaaagaattt ctgaccactc
     1801 aacaaatata gtaaaacaag tactaaaact taaacaaaaa atatagtgat tatttttata
     1861 tataatctta atcaatgaga agtaagaagc tatagataat gcttatatca tttatggttt
     1921 cttatttttt atgtaatact caaattttta tagtattctc ttattattaa acaatttctg
     1981 cctagaatat tccatatata tatgacttct ttcataaaac ttgaactgcc cataactaaa
     2041 gtcacaagta tacataacta ttttttaatc aattataacc aatatttgtt ttatttatct
     2101 tcattttaat tcttcattca ataatgtaat aagattattt tttatttgga tactattata
     2161 tcatctaaat atgttattat atatttggat attttcatat taataggagg gattctacaa
     2221 tgataaatta tatggtagaa attatatata gcgaaaaaat aagcgaagta gaaaacatgt
     2281 tgaaaattga aaatatacaa tatgaattta cttctcgtga tggctttata ttttttggtt
     2341 tttatgatat gacagacgca tatagatttg agactttatg ttcagatgaa aaaattcgat
     2401 ttaaaagaat ataatagata aaaaagtaca tatttctaaa ctatttgttt ttcaaattta
     2461 ggttgtgttt taatgtagtg ttgatattat aaacaatttt aataataaaa aagtagggac
     2521 attatattaa tatgctccta ctttttactc atacttatta atattattta tcaaaattac
     2581 cctacaaact ggaatttatc attctttcat aattttaaac tatcatattt aaaatatata
     2641 aaattccaca tgatttttgt ataaacataa tatcaaccat tatccaattt aaaaatattt
     2701 tttagtaatt tccatttcat tttctcttaa catattaatt aaatttttat atgtaaatgg
     2761 tgtatttaca tattatttaa tatccaacaa ttatataaag aaatgtgctc tttgccttat
     2821 aaagaattat aaagccaatt cgttttatta tattattttt ctttatacct taaaaacttt
     2881 accttcaaat tcttatttat ctatatatta taaaaatgtt cttaacctct tttacgagat
     2941 tttcttgaca tattatcagt tagataaaat aattaaataa tataatcagc aagtatgttt
     3001 tttactcttt taagtaaatt tccacctgca ccccaatcaa tttttaaaat tccaattgca
     3061 ctgcctgttg aagtagcttt aataactgtt tttcctgtgg tattttcata aactactatt
     3121 aatcctgcat cactattatt tcttgcataa tacttctcaa aagttgtaat tagtataaat
     3181 ttatcatctc ctaaattata agaatcacta aaaactaaat ctgcatcaag attttctttt
     3241 atcatttttt gtgcctctaa gggtgtaatt tttacagtaa atatttctgt tgccattttt
     3301 tatctcctca taagaattta ttttagacaa aacataatct ctagtatttt ctgatatatt
     3361 tatcaaacaa tatccctatt cctcctaaca gagctaatat agaaccaaaa tttctatatt
     3421 gagaaataga ttggtctaga taaatattat attggtcagt atccatgatt ccaccatttt
     3481 gttgaataat atgctctgca ctaaattcac ctaaacctat acaacagaat aatactacta
     3541 ctccaattac aaaagcaaat atacatattg ttaatttaaa atccttattt tccatagtta
     3601 tccctctcat aagctaaatt tatttgatta tttttactag attttatatt actcttatta
     3661 tacaatacaa catatattta catctatttc caaaattgta acctttatag ttttatatat
     3721 ttaaattttt taaa
//
LOCUS       DAEORZ010000242         3727 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEORZ010000242 DAEORZ010000000
VERSION     DAEORZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.248"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            97..603
                     /locus_tag="KRH39_003707"
     CDS             97..603
                     /locus_tag="KRH39_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBG8513573.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            731..1747
                     /locus_tag="KRH39_003708"
     CDS             731..1747
                     /locus_tag="KRH39_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG8513574.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2101..2805
                     /locus_tag="KRH39_003709"
     CDS             2101..2805
                     /locus_tag="KRH39_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBG8513575.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDTDNKEVSVD"
     gene            3100..3579
                     /locus_tag="KRH39_003710"
     CDS             3100..3579
                     /locus_tag="KRH39_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG8513576.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILAVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT         1541 a          340 c          635 g         1211 t
ORIGIN      
        1 taaaatcttc ctatatcata taaaattaag tattgattag tctattacat aaatatagaa
       61 tataaatata tccatttaat aataaggaga agagatatga aagagtatgt agttgtggat
      121 ttagaaacta caggattaga tccatataaa ggttgtgaaa taatagaaat aggaataaca
      181 gaaataaaaa atgaacaaat tgtaaaaaac tattcaaggt tgataaaacc taaggggata
      241 atttcttcat ttataacaga attgacacat ataagtaatg aaatggtaga aaatgaagaa
      301 ccactagaat tagttttacc tagatttaga aaatacatag gtgatagaac tataattgca
      361 cacaatgcga aatttgattt aaaattttta aattactatc ttagaatgtt agatttagaa
      421 ccaataaaca attatatatg tacagtagag ttattaaaaa aatgtaaaag ttataaagga
      481 aaaaacaaga aacttgaaac agcttgtgct tattacaaca tagagaatat aaatgctcat
      541 agagctgaca gtgatacatt agcaactgca aaactatttt tgaaaataaa agatgaatat
      601 taattataaa tatagagaaa attagtgaaa atatccttta taaaattgac ccttaagctt
      661 acaattttgt tctttgattt attttgtaga acaatgtata atgtacctta tatgaagaag
      721 ggagactaaa atgggttatt acaatgataa tgataataat aaaataagac gtaaaaaaat
      781 tagtaacaac aaagatagta ataaaaaatc taaacattct tatacttcgg caaataaaaa
      841 aaataaaagt cctaatacaa gttccagtaa aagaatgagt gaaaaagtag attcacagaa
      901 tcttataaaa agagatagag atagttttac aagagataat gtaagacctt taaatacaga
      961 ttacaatcaa agagaaagaa gaagagtcga gtataaacaa agagagaaaa aattaaaaag
     1021 taaaaaaaga aaaaaacaaa aaaggattag aatagttaga gcattattat ctattatgtt
     1081 agttgcacta attatatttt cagtggcagg tgcaatattt acagtttcag ctataaaagg
     1141 gtcaccagaa gttactaaaa agttaattag gtctaaatat ataagtagtg aggttgtaag
     1201 tataaaacaa atgccaaatg atttaaaaca tgcaatagtt tccatagaag atgagagatt
     1261 ttataagcat aaaggagtag atgttatttc tctagttagg tcagtattaa acaatgtgtt
     1321 tacagataca acacaaggtg caagtactat agatatgcaa gtttcaaaaa acctgcttac
     1381 tagtaatgat aaaactatga agcggaaaat aagggatatg tataatgcta agcagatgaa
     1441 taagattatg actaaggatg aaattcttga agcatattta aataatatgt atctaggtaa
     1501 aaatgcatat ggagctgcaa agggagctga agtctacttt ggtaaaaagg ttagtgaatt
     1561 aaatttagct gagtgtgcta tgttagctgg tatgacaaat aatcctgcaa ggtatattga
     1621 ccatggagag gctaagaaga gacaagtgat tgtactgtat aagatgcatc agcttggtta
     1681 tataactgat aaggagtata gaatagcaaa agctgatgac actccattta aatcagaaat
     1741 tgattaaaat tgtaagaata tagtattttc aatagtttaa acaaacgaaa atgtaataaa
     1801 aaaggtgatt tgccacccat ttgccaccat aattacaatc taatggcaaa ttgttttaat
     1861 gtacaaacta gataatagaa tattgtttta aatgctatat tatgctaaaa tttaattgta
     1921 ttttgtaaat taaaaaactt ataatttagt tttctagggt tccgcatttt gtaatggtca
     1981 ggtccaagag aaaacgcaca attgttgtgt acacggaagg ataaaagcct gggagatatt
     2041 taagaatatt tctataggct tttttatttt tacatatttt aaatttgagg aggttttcta
     2101 atgaaaaaat ttgttattga ggatgcattt tggaatctat ttcctagtgc aaaaattgga
     2161 gttgttattt gtaatgatat agataactct ataaaagatg aagactataa gaaaataatt
     2221 ttgaagggag aagaagaagc attaaaatac ttggaggact cagaatttag caacaaccaa
     2281 gtgattaaag tatggagaga agcatttaaa aaatttaaaa cgaaaaaagg agcaaggtct
     2341 tctattgaag cattattaaa aagagttcat aatggaaata atataggaac tattaaccct
     2401 ttagtggata tctacaattt tatctcatta aaatatgcat taccttgtgg aggggaggat
     2461 atagataaat ttataggaga tattaggcta actagagcaa ttggtaatga ggagtttatc
     2521 ccactaggga cagatgaaaa ttcatttcca tatgaaggag aaataattta taaagatgat
     2581 ggtggagcaa tttgtagatg ttggaattgg cgtgaagcag tgagaacaat gcttactgaa
     2641 gatactaaca atgccttttt atgcattgaa ttagtagatg aaagtagatt taaagaattt
     2701 gaaaatgctt taaatgaatt agctaaaata gttcaagaaa aattgggtgg gaattgtaaa
     2761 atttcaattt tagatactga taataaagag gtatcagtgg attaatatgg aggtgctgtt
     2821 gtaatggact attctagtta taaagatact tatatttagg gtaagaaggt ctattttaga
     2881 tgtaacgatt atcaaaggat ataatataga aaataaaaat gtagaatata aaaagccttt
     2941 aaaataagaa aaaaatgtat tttaaaggct tttattatac tcttttttat ttgcataatt
     3001 aacaataaaa atatattgtt aattataatg tttttattga aaaacgatat aatataatgt
     3061 attatactat atataaaata ttttaaagga gttggcatga tgtctaataa acttataaaa
     3121 aatttactaa gtggtatatt acaaatttta ttttttcttt taggattagt aattgttgca
     3181 ggaggattta aaagttttat gtacctctgt tttagtggag aagcaactct acaaggcact
     3241 atttatggaa tattaatgtt tattttagct gtatcatatt ttatcattat aaaatcattg
     3301 atagaagtat taggtagttc tgaatatagc ctatttgtaa aagagaatgt aaaaagattt
     3361 aggattattg gatatctttt gcttttaaac tctctaattg agtttatttc aacttttggt
     3421 actactggaa aaggaatgag attcctagat ttaggatttg gattttattt tactgttcca
     3481 gtatttgtat attttataac ttctcttatg agttttgtaa ttgcagatgg atttgtaaag
     3541 gcaataaaga taaaagagga taatgattta actatttagg tgtgacactt taatatatag
     3601 gagataaata accagaaaag agtgattaac tagtaattta aaataaaata tattatggta
     3661 taatacataa actataatgc caaaattaaa aaattataat actaaaatat aattatgttt
     3721 taattaa
//
LOCUS       DAEORZ010000243         3694 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEORZ010000243 DAEORZ010000000
VERSION     DAEORZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.249"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            629..898
                     /locus_tag="KRH39_003711"
     CDS             629..898
                     /locus_tag="KRH39_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG8513577.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(1116..1361)
                     /locus_tag="KRH39_003712"
     CDS             complement(1116..1361)
                     /locus_tag="KRH39_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBG8513578.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            1789..2487
                     /gene="tcdC"
                     /locus_tag="KRH39_003713"
     CDS             1789..2487
                     /gene="tcdC"
                     /locus_tag="KRH39_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HBG8513579.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            complement(2822..>3694)
                     /locus_tag="KRH39_003714"
     CDS             complement(2822..>3694)
                     /locus_tag="KRH39_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C80"
                     /protein_id="HBG8513580.1"
                     /translation="FAPANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRT
                     IDGKKYYFNTNTAVAVTGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQI
                     GVFKGPDGFEYFAPANTDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDG
                     NRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQ
                     AIRYQNRFLHLLGKIYYFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIY
                     FFGVDGVKAPGIYG"
BASE COUNT         1344 a          457 c          528 g         1365 t
ORIGIN      
        1 taaaaaatca ttcaatatat taaataaata tttaacttta ataatctata tttaattata
       61 gctataacat agaattatca aatatattga atgaatattt gactcttgga atgtgtaatt
      121 aaattaagtt tggttattcg atggaaggca aatttgatat aaaactccat ttaaagtgcc
      181 agataaaaac tttatggtta tataaaagtt aaaagtaatt tatagtgtta tagaatattc
      241 aaagggattt aaagtaatct atggtgtttt gaaatattca aagggattta aactaatcta
      301 tagtcttgta gaatattcaa agagatttaa aacaatctat aatgttgtaa aatatattca
      361 aagggattta aactaatcta tagtcttgta gaatattcaa agagatttaa agcaatctat
      421 aatgctgtaa aatatattca aggggatttg aagtaattta ttgtgttttg aaatattcaa
      481 aaagattttt ggaatagttt ttataaattt accagtacta ataattttat gtttaacatt
      541 tagatgacct aaaatcaact atttgcactt ttgtattcat ctaaaatcat tttataatat
      601 ttttaaataa tggaaaggat gtttgaatat gtttgaatca agatgtggtg tctgttgtga
      661 cagttgtaca aaaaaagaac aagtaaattg cactggttgc ccaactatgg aaaaaccatt
      721 ttggggtgga gaatgtaagg ttaaaacttg ctgtgaaagt aaggaactta atcattgtgg
      781 tgaatgtgat actttccctt gtgatatgct tttaaataag ggaaaagacc aaggttttga
      841 ccctatggta aatattgagc aatgtagaaa atggcttgaa gaaacagttt ctaattaatt
      901 gttttagaat gctttgttaa gtaatttatt taatcctttg ttaggtagta tgtttagtca
      961 tttgttgatt ggtatattta tacatttatt gatgggtata tttagccata caaatagatt
     1021 tacataaaat tcacgactaa aaaagagttg agtattagtt tttattattt aaaacggata
     1081 ttcaactctt ttttatgtct aacttttttg attttttaat ctttccaata ccaccacttt
     1141 aatttttctg gttctggatt ttcagttgat gcaaaatata ctgcacatct gtatacatat
     1201 aactgacatc tgtctatgtg ctgtccacgc attaaacatt ccttttcata catttgtgct
     1261 gggtttgcat ctttaagtga tttaatagtt gtatatccta acatgattaa agatttttct
     1321 gttgctttcc ctacccctgg aattttttgt agttctccca tacttcacct tctttctggt
     1381 atattctttt tatattatac ctattaccag atattttttt attatagtta atatttaata
     1441 tttaattttt tattatatca atttatttat gctctttcat atatatatat attttaccac
     1501 ctctaaatca ctgagtcact taattacatc ataatatagt tttatacaaa taaaatactt
     1561 tatgtttaat ttaatatata aaattcacct tcaagaaaat tatattataa tctgacattt
     1621 ttatcttatt ttttcaaaat atattgaatc ttctcacttt attttgtaaa attatgctta
     1681 ggggaaatat attttaggaa aatatgaata tataattttt agtcaactag ttattttaag
     1741 tttttaactt ttaaaataaa atatatctaa taaaagggag attgtattat gttttctaaa
     1801 aaaaatgagg gtaacgaatt tagtaatgaa ggaaaaggaa gctctaagaa aataattaaa
     1861 ttctttaaga gcacaaaggg tattgctcta ctggcattta ttttaggcgt gttttttggc
     1921 aatatatcct caccagcttg ttctgaagac catgaggagg tcatttctaa tcaaacatca
     1981 gttatagatt ctcaaaaaac agaaatagaa actttaaata gcaaattgtc tgatgctgaa
     2041 ccatggttca aaatgaaaga cgacgaaaag aaagctattg aagctgaaaa tcaacgtaaa
     2101 gctgaagaag ctaaaaaagc tgaagaagct aaaaaggctg aagaacaacg taaaaaagaa
     2161 gaagaggaga agaaaggata tgatactggt attacttatg accaattagc tagaacacct
     2221 gatgattata agtacaaaaa ggtaaaattt gaaggtaagg ttattcaagt tattgaagat
     2281 ggtgatgagg tgcaaataag attagctgtg tctggaaatt atgataaggt tgtactatgt
     2341 agttataaaa aatcaataac tccttcaaga gtattagagg atgattacat aactataaga
     2401 ggtataagtg ctggaactat aacttatgaa tcaactatgg gtggaaatat aactatacca
     2461 ggaatagctg tagagaaaat taattaatat tgttttgtat tatagttaat attttatatt
     2521 atagtcaata tgtttaaaga tgtttttata attgcaaata aacagttaca aggctctaaa
     2581 ttagtttttg cttttagcat attatctatt ttttatcaac tattaattat ttagtattaa
     2641 tatttccata tatgaatttt attataaaat agtcaagaat aatagattat taaatgatag
     2701 aaaaatttta actaaaagtc atgtattaca ataacacatg acttttaatt aaatctcaat
     2761 atttattata taaaaataat ttcttagtag cacagggata attttttatc aaacatatat
     2821 tttagccata tatcccagga gcttttactc catcaacacc aaagaaatat ataacaccat
     2881 caatctcgaa aagtccacca gctgcagcca tagcagtatc aggcataaag taatatactt
     2941 taccattaat agtttgccat ccagtaactg cttttgaatt attaccaaag taatatattt
     3001 ttccaagtaa atgtaggaat ctattttgat aacgtatagc ttgaccttct atattgttag
     3061 catccgtatt agcaggtgca aagtattcaa atccattaga ccctttaaac actcctatct
     3121 gaggtaaacc atttctaaag taaaaatttt tattatcaat agttttataa ccattcgcac
     3181 ccatagctgt attaggctcg aagtaatatc tattaccatc aatagttacc caaccagtag
     3241 ccgcttttga attattacca aagtaatata tattgtcatg taaatatagg aatctatttt
     3301 gataacgtat agcttgacct tctatattgt tagcatctgt attagcaggt gcaaagtatt
     3361 caaatccatc aggtccttta aacactccta tctgcataat accatcagta ttaaaataaa
     3421 aatgtttacc actaataatt gtataaccag ttgaagctat agaagtgtta gtattaaagt
     3481 agtatttttt accattaata gtttgccatc cagtaactgc aacagcagtg ttagtattaa
     3541 agtaatattt tttaccatca atagttcgca atccggtaac tgcttttgag tcactaccaa
     3601 agtaatattt tttaccattc aaagttaaga atttattttg gtaaagtata gcttgacctt
     3661 ctatgttatt attatgagta ttagcaggtg caaa
//
LOCUS       DAEORZ010000244         3643 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEORZ010000244 DAEORZ010000000
VERSION     DAEORZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.250"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            215..1591
                     /locus_tag="KRH39_003715"
     CDS             215..1591
                     /locus_tag="KRH39_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBG8513581.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1926..2597
                     /locus_tag="KRH39_003716"
     CDS             1926..2597
                     /locus_tag="KRH39_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBG8513582.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2786..3583
                     /locus_tag="KRH39_003717"
     CDS             2786..3583
                     /locus_tag="KRH39_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBG8513583.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1374 a          403 c          680 g         1186 t
ORIGIN      
        1 tatgtaaaaa atatagatgt ttttggtgca atttaaatta aaagatatat ataaaaattt
       61 aaaatattcc aaacagaaat tataaaaatt actatcattg tctttataca atatttatac
      121 tataatagaa taataattta gaggaaagct ctaaaaataa acaaattaag ggtcttaaca
      181 gtagtgattt tatgttaagg aggtataaag aaagttgaaa acaatagtta gaagacttat
      241 tctctacgca gatggaatgc gtcctactca aataatggta tctgggtttg ctgcgattat
      301 tgtaatcgga gcattgttgt taacactccc gatagcgtcg cagagtggag agagtatagg
      361 cttattaaat gctctattta cggctacttc agcggtttgt gttactggtc tagttatggt
      421 agatacagca acttattgga gtttatttgg acagatagtt ataataacat tgatacaaat
      481 tggtggtctt ggatttatga ccgtagccac aatgttttca cttatggcac gaaaaaaaat
      541 acaactaaga gaaagattat taatacaaga gtcattaaat caagctgatt tatcgggact
      601 tgtaagactt acaagatttg tattaataat aaccataaca atagagggaa taggtgcact
      661 ggttttatca actgtcttta ttccacaatt tggtttgtct aaagggatat ggtatagtgt
      721 atttcacgca atatcagcgt tttgtaatgc cggatttgac ttgatgggca gtgtaagtgg
      781 cccatttaca tcgttaaatt cttatgtaaa caactttaca gtatcaatga ctgtttgcgc
      841 acttatagtg cttggagggc taggattccc tgttgtactt gatattgtaa gaaaaagaag
      901 attttcaaaa ttaaatgtgc attctaaggt tgtattgttt tcaactgcta cacttatttt
      961 tgtgggtgca ttatttattt ttcttataga atttaatcaa aaagctacta tggcggactt
     1021 gcctttaaag ggaaaagtac tatcagcaat atttcaatca gtaacagcaa gaactgctgg
     1081 ttttaatacg cttgatttag ctactttacg agaaagtagt gtatttgtaa tgataatttt
     1141 gatgtttatt ggagcatcac ctgcatcaac tggtgggggt ataaaaacaa caacactagc
     1201 tgttttaatc attacagtta gaagttttct atcaggaaaa tcagatatag aagcttttga
     1261 aagaagatta gctccatcaa ctataaaaaa atctcttggt atatttgtaa tcagtatatc
     1321 agcagttatt tttggaacat tgattatttc aataactcag cctaatttta cattagttca
     1381 atcagctttt gaggttacat cagcacttgc tacagttggt tcaagtttag ctggaactcc
     1441 aaacttaaat gctttaggta agattataat aataatcttt atgtttatgg gtagagttgg
     1501 ttctcttact ttatttatgg ctatactttc tggtggtaga agaaagagtc aaccaatcag
     1561 atatgcagaa ggtaaaatta tggttggtta agttaccaag gagtaatata aatttataat
     1621 ggatttagag tagcactgta aatctgttga taaaataaat gagcctagat gtaattatat
     1681 gaagaaataa aatcgactaa tgaacaaaat taattaatga aataaaaaaa cagggaaata
     1741 aaatacaaat aaaaaggtaa atcaaaatta aattaatctg tgtatataaa attatatatt
     1801 gtgtataagt tttgtatgtg taaagtatat aagttttgta tgtgtaaagt atataagttt
     1861 tgtatgtgta aacaattaat ttaagatata ttatataaat tgtgtattag ggaggaagtt
     1921 tcgagatgaa gcaatatata gtcattgggt gtgggagatt tggaagttca gttgcgtcta
     1981 ctatgcatct tttaggacat caagtaatgg caatagacaa aaatgaagat tcagttcaaa
     2041 gtatatctga caaggtaacc cattcactta tagtggatgt tactgatgag caagcgttaa
     2101 ggtcattagg tttaggtaac tttgatgtag cagtagttgc aataggttct gatataaggg
     2161 catctataat ggcgactctt atagccaaag aaatgggtgt agagttgata atatgtaagg
     2221 caaaggatga attacaagct aaagtgcttt ataaaattgg cgcagataga gttgtatttc
     2281 cagaaagaga tatgggagta agagttgcac acaatttagt ttcggataat atattagacc
     2341 atattgaact tgacccagag tattcaattg ttgaaatcgt aactccaaat agttgggttg
     2401 gcaagacact tatagagctt gaattaagag ctagatatga gataactgta cttgctataa
     2461 aaacaggtaa aaatataaat gttacacctt ctccagatga ggaacttaca gccggaagta
     2521 tcctagttat aatcggtcaa aatactagta taacagcgat aacatctgga aataagggga
     2581 taattagaag aagataattt actatttaat atatatttaa ttgcaatgaa agtagatagt
     2641 atcatataat tatgaatagt tatatgatac tattttttta atcgaaggta gtatttattt
     2701 gtaagattag ataaagagaa gttaaattaa aagtaaggag gctgtgctaa tataaaaatt
     2761 tataagttat tagcatttag ataaaatgat tacaaatata aacagtaagg ataatgaaaa
     2821 gttaaagtat acaagagcac tattaaaatc aaagaatagg aataaagagt caaagttcat
     2881 aatagaggga tacagaatag taatgcttgc acttgaatgt atggcaaacc ttgattatgt
     2941 atttatcaat gaagaatttg aaaataagaa agaacatgta aaactattag aagatttgga
     3001 taaaaaaaac acaaagatat acaagactac taataaaaac tttaaagaat tagtggatac
     3061 agaaaatact caaggaataa taggtgtagt ttcatttaag aaaaaaaaat taagtgaaag
     3121 tataaataaa aaagataaat ttgtattgat tttagacaga atacaagacc caggaaatat
     3181 ggggactata ataaggactg ctgattctgc tggagtagat gccataatag cactaaaggg
     3241 atgtgtcgat atatacaacc caaaagtaat taggtctact atgggttcta tttttgatat
     3301 gaatataatt gatgcttcac aagatgaaac tgtggacatg cttaaatcat tggattttaa
     3361 tatagtttca agttacttaa atacagaaaa tttttatgac aaaatagatt atggttcaaa
     3421 agtagcattg gtgataggaa acgaagcaaa tggaataaat gaagaacttg tatcaaagtc
     3481 tgatattttg gttaagatac ctatatatgg taaagccgag tcgttaaatg ctgcgataag
     3541 ctctgctata ctgatgtatg aaataaaaaa atacttaatt taatgtattg taataaatat
     3601 aatgtcatgt tataatcttg aattaaatag tagagtatca aaa
//
LOCUS       DAEORZ010000245         3577 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEORZ010000245 DAEORZ010000000
VERSION     DAEORZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.251"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(6..1604)
                     /locus_tag="KRH39_003718"
     CDS             complement(6..1604)
                     /locus_tag="KRH39_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG8513584.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTVILVNTDNSIVDGLSASGLSGVVKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSISSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRDADEVTSLGGMDKKVVDQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGDDLKANIKGNVDTNKAGIYVLNISVVDNLGQKSEISVNVKVVVNTSTKDPNS
                     YEFKAMVSNEMYDLVNSYRKEKGKKGLRELDSLAGMANAWSKYMDDKKVFAHEIDGKN
                     AAEVFFGFGARSGENIAYLPMNVKSVYNFKDAKDMAKSIFDLWKKSSKYNENMLKEEF
                     YSFGFGMHVSSKGEVNATMEFLNS"
     gene            complement(1823..3424)
                     /locus_tag="KRH39_003719"
     CDS             complement(1823..3424)
                     /locus_tag="KRH39_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBG8513585.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADRQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIQVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEIDEKVIQQCINASKSNGQPPTITVGSTEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
BASE COUNT         1061 a          742 c          373 g         1401 t
ORIGIN      
        1 aaaatctaac tatttaaaaa ttccattgtc gcattcactt ctcctttact agaaacatgc
       61 ataccaaatc caaagctata aaattcttct tttaacatat tttcattata cttagaagac
      121 ttcttccata aatcaaaaat agacttggcc atatcctttg catctttaaa gttataaaca
      181 gatttaacat tcataggtaa atatgcaata ttttcaccac ttcttgctcc aaagccaaaa
      241 aatacctcag ctgcattttt accatcaatc tcatgggcaa ataccttctt atcatccata
      301 tatttagacc atgcatttgc cattccagct aaagaatcta attctcttaa acccttctta
      361 cctttttctt ttctatatga attaactaaa tcgtacatct cattagacac catagcctta
      421 aactcataac tattagggtc ttttgtactt gtatttacaa ctaccttaac atttacacta
      481 atctcactct tctgacctaa attatccact acacttatat ttaaaacata tattccagcc
      541 ttattagtat caacatttcc tttaatattt gccttcaaat catctccata ataatcagta
      601 gcaactatat tcaacatact attatcaaac ttatccccta cagaaatctc tacatcatta
      661 gcagtgattg taggcattgt atttttatca gatgcagaac taatacattg gtcaacaacc
      721 ttcttatcca ttccacctaa agaagtaact tcatcagcat ctctaagcac gctcttatca
      781 cttccatcat taacaagtac tataggtttg tccttagcca acggagaacc agccacagca
      841 tcaactaact tatacccttg agaaacataa aacttctttg ttcctttata aaagcgttgt
      901 ataaccttct tattagtctc aaacctatcc ttaccagcaa tcctaacaga attagtctta
      961 ctcacaagtt cattactcat tatctcctct gacccaagag aataacattg aacaccatca
     1021 accttaaaag gtatactctt tccatcagta agtataacag gaactccatc tctagcagct
     1081 acagaagata tactcattgc atcagcctca cctgtgtatc catttacaag aaaaacctta
     1141 tcaccatcat ttactggttt aatagctgaa atctccttag ctataagata gctagtcttt
     1201 attctatctt ccccaccaat tcttttaact tcaatccctt tattcttaag ttgattttct
     1261 acagatttac ctattgtatc ctctgtacct ataacataag cattcttaac atcctttagt
     1321 cttttttcaa catctgttgg tattttatct ctttgcacca acattatagg agcttttaca
     1381 actccagata atccacttgc acttaatcca tctactatag aattatcagt atttactaat
     1441 atgactgtat catatatttg tttatctgct atcaatgcag aagtctcata tctatctttc
     1501 ccttgtatct tttctatact tgaaagtgca ttgatatttg gtgctcctgc cattattaat
     1561 gatattgtca atcctattga taatactctc ttatttacct tcatcaaact cctcctacta
     1621 atttttttat ataattcagc taatcataaa ttacatcctc agtagtgatt ccctcaaaat
     1681 ttataaacta ctaaaacaaa aaacacctaa aataccataa cctgtaaagc aataacaaaa
     1741 cagattacaa aaagtacttt agatgtgatt ttcattttat aatatcagta aattaacaaa
     1801 aaaacaagat ttatggaatt ttttacaagc taccttcatt gccatttaaa aactcctgag
     1861 tagcatgcac ttgaccatca gataatatat aaagcccaaa tccagtacta taaaactcat
     1921 tactaagcat gtttgcattg tactctggag acttcttcca cacctcaaat atagcctttg
     1981 ccaagtcttt tgcatcttgt gtcgtttgaa cgttcttaga atctatataa atatatgcta
     2041 tattttcctc acttctcatt ccaaactcag aaaatacttg tggagcattt tttccatcta
     2101 tataatgtgc aaatactttt ttatccatca tatacttaga ccatgcattt gccatacctt
     2161 ctagcctact agatactacc aaaggctctt tgcctttttc ttttctatag gaattgatta
     2221 agttatacat ttcagtagat accatttttt taaattctgg gctgttgtaa tcatgagatt
     2281 tatcatccaa cacttttatt tctactcttt ttcctgtact ttttccccat tcgtctgtag
     2341 cttttaatgt caatatgtat gtacctactc tatttgtatc tataaaccca tcaacttcta
     2401 ttggtaatac cttcccagtg ttatctttag ccactatatt taacttgcca gtgtcaaact
     2461 tttcaccctt atatatctct gtacttccaa ctgtaattgt tggtggttgt ccatttgact
     2521 ttgaagcatt tatacactgt tgtattacct tctcatctat ttcacctaca gaggttatat
     2581 tcttagctcc ttctaataca gttttatcgc ttccatcatt tactagtacc attggagagt
     2641 ttttagtaag tggtgcggct gctattgcat ctactaattg gtatccatct gatacataga
     2701 atccatctgc actattaaaa aagtagtcta ttacgttttt gttagtttca aatcggtcag
     2761 ttccttctat tcttactgag tttgtatttt taactagagg attactcatt atctcctctg
     2821 aacctataca atatgattga atgtttttta catcaaaagg cacactcttt ccatctgtaa
     2881 gtatgattgg agctccatct ctagtagcta ctgatgatac actcattgca tctgcttctc
     2941 cactatatgc attagttaat agtactttat ccactttttt tatagttgct atttctttag
     3001 ctattaaata gcttgttttt agtctatctt ctccaccaat tcttttgact tgtatatttt
     3061 tagaatctag ttctttttct actgatttac ttatagtatc ttctgtacct attatatatg
     3121 cttttttgat gttttttaga catctatttg tgtctgctgg tatcttattt tgttgtgtaa
     3181 atagtattgg agcttttgta gcccccgata atccacttgc acttaatcca tctgcaagtg
     3241 atttttctgt atttactaaa attactgttt cataagtctg tctatcagct atctttgctg
     3301 ctgtttcata tctatcttta ccgtatattt tttctactac tgatgatgca tttacacttt
     3361 tgaagtttac taagattaat gatactgcta gacctaatga taatattttt ttgttcattt
     3421 tcatatcccc ctgatgattt ttatatttta tatttatatt gtgattattt tccatttatt
     3481 ttctctttat tttccatata ttatagagta tatatgatat gaatatatct cgtctatata
     3541 ttagtgtttt attttattaa atttaaaaaa ttatttt
//
LOCUS       DAEORZ010000246         3556 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEORZ010000246 DAEORZ010000000
VERSION     DAEORZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.252"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            125..1396
                     /gene="sleC"
                     /locus_tag="KRH39_003720"
     CDS             125..1396
                     /gene="sleC"
                     /locus_tag="KRH39_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBG8513586.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(1904..2560)
                     /locus_tag="KRH39_003721"
     CDS             complement(1904..2560)
                     /locus_tag="KRH39_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513587.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
BASE COUNT         1251 a          612 c          380 g         1313 t
ORIGIN      
        1 atacaaatat actcaaatac tcaaaaaaat ttaaatttat atctatatta tttaacaaat
       61 ttcaagctat attcatatct tgaaatataa gggaactaaa ttcatttaaa gaaagggtga
      121 tttgatgcaa gatggtttct taacagtaag cataattgat gctactaaca atagacctat
      181 tcaaaatgca gtagtaaaca tatattcaat gtctaatggt agccaaagct catcaacttt
      241 ataccaaaac ctaagaagta atgaatctgg tcaagtgact ggtttagtat taccagcacc
      301 agatgtagat tactctctac aaccatctga tgttagacct tatagccagt atatagtgga
      361 agccatagca gatggatatg agacagttgt catagaagga acacagttgt tagctacaat
      421 tgaagcaaga caaggagttc ctatgtctcc tagaacaaga agtaagcgtt ctttctcaag
      481 acaaagtgag ttgatttttg acataggaga acatactcta tatggtactt atcctcctaa
      541 gattccagaa agtaacttaa aaccattacc acctcctact ggtttcgttg tattagataa
      601 ccctgtagtg cctgaattta tagtagttca tgatggtttg ccagaagatt catcagctcc
      661 aaactattgg attccattta aagagtacat taaaaacata gcgtcatctg aaatatattc
      721 aacttggcct gaacagacaa tctatgcaaa tgtaatagct ataatatcat ttactttaaa
      781 tagagtcttt actgaatggt atagaaataa aggatataac tttacaataa cttcaacaac
      841 agcatacgac cataagttta taaataatag aaacttattt gaaccaatta acgtagttgt
      901 agatgctatt tttaatactt ttataaaaag acctcctaca tcaagacagc cactattagc
      961 acaatattgt gatggacaaa aatcccaatg tcctgaccaa atgactcaat ggggcagtaa
     1021 agacttaggt gaccaaggat atgactatga gagtatacta agatatttct atggagatga
     1081 gatagtattt gaaagagcac ctatagtaag tggtgtacca gtgtcattcc ctggtactac
     1141 actacaagtt ggttcttctg ggcagtatgt tagaaccata caaaatcagt taaatgctat
     1201 atctaattct tatccagctg taccaaaagt tatagaagat ggtatctatg gaacagatac
     1261 agaaaacgct gttaagatat tccaaggaat ctttggttta cctcaaagcg gagtagtaga
     1321 ttttaaaaca tggtatgaaa tatccagagt ttatgtagct acaactagaa tagcttcttt
     1381 aaatccttta atttaaagct tgatttagaa cataaactca aattattgaa taaaattatt
     1441 aatttaaaat aaaaatggct gttatgaact aatatatacc ataagtatta cttataagta
     1501 ttgtagtctt aacagccatt ttttagttat aacttgtttt tttagaatct taaaacttta
     1561 aatgggggat tttaagcttc ttaaaaacag tgcttagcaa cttagatttc tttaaaatat
     1621 tatatttatt tttaagttta ataaaatgct attctaacga aaaacctact ttttatttaa
     1681 aatctcactt attatttaaa atgctacttc tcacttagag tattactcct tattatttaa
     1741 aacactgtct atcacttaga gtattacttc ttattattta aaacactatc tatcacttag
     1801 aatattactt cttatataaa ttattattta ttataaatac aaataatatt aatttaaaaa
     1861 gttattccta atttaaataa tactactatc ttcatcagtg aatctaaact gcaactttag
     1921 acttattagc taaagccaat attagaccta aagctataga tgcaaatgca gaagcaatta
     1981 ctctatactc agcagggaat aaaaatacaa ttgtatgaca tggtatccag aaaaatggta
     2041 tagtttttaa aacactaaaa ccaaggtaag cattccagtc cacaatattt accacatctt
     2101 taagttttac cttaatcccc tttccaacct catatttttt atctataaaa cagtccataa
     2161 ccttatggaa taaaaataca gttggtgaca ttagtatatt catagttgca ctcttaaata
     2221 gagcatttgc aaaagctatt ccctcaaatg gaagcatacc atccacttgt gcttgaccta
     2281 cacctgatga gtagattgac attccatatg tggtaactac acctattatt ccccaaacta
     2341 ttgctctata aaatactcct actggtattt tccaatcctt atacaaaatt ctagcaccaa
     2401 gtaaatctcc cattgtagct aatacagcaa actttaaaaa acctccaata tatggatgtg
     2461 cacctgttgc ttccataaat acttctctac tacttggtat tgctaagaca gcaataatac
     2521 aaataagaac tccactccat ataaaatcgc cttttttcat atttataaat actatttaat
     2581 atataaatag ttctcctttc tattaaagat ttatttaatt tacaatctat ctacaattat
     2641 ttattaatca cttattatta gatattcatt ttccatattt aaatttattc tatcccttta
     2701 tctatttatt ttcttactac tctatctacc attatctata tatttatttt acgattactc
     2761 tttcacccta accctttaat tatctatttt tttctgatat tcttatctac catcatccat
     2821 atatttattt tacgattact ctttcaccct aaccctttaa ttatctattt tttctgatat
     2881 tcttatctac cattatctat atatttattt tacgattact ctttcaccct aaccctttaa
     2941 ctatctattt tttcttacta ttcttatcca catcatccat atatttattt tacgattact
     3001 ctttcactct aaccctttaa ctatctattt tttctgatat tcttatccac catcatccat
     3061 atatttattt tacaattact cttttaccct aaccctttaa ctatctattt ttttctggta
     3121 ttcttatcta ctattatctg tatatttatt ttatgattac tcttttaacc taccccctat
     3181 actttatagt aatatgtttt taaccttttt cattattccc aatatgcttt ataactacaa
     3241 atactctccc cccttaaaaa taataataaa attaatcaat aaatttataa tcatattatt
     3301 actctatgta ttaagcaatt actatgccaa taaactttga tttttttaaa caaatcttaa
     3361 aaatcctaaa atgctaattt tctttaactt ttaatgaatc taatagataa catttaattt
     3421 tcgttatatt tttcagaata tttctattat tatttttaag aaaaaaaaat aatgttaaca
     3481 tttcttaaca tacattaaaa aatgttaaca ttataaattg ggtttaatac ttttactttg
     3541 actcgattta ctttta
//
LOCUS       DAEORZ010000247         3530 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEORZ010000247 DAEORZ010000000
VERSION     DAEORZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.253"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            187..522
                     /locus_tag="KRH39_003722"
     CDS             187..522
                     /locus_tag="KRH39_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8513588.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            659..1936
                     /locus_tag="KRH39_003723"
     CDS             659..1936
                     /locus_tag="KRH39_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBG8513589.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            2057..3445
                     /locus_tag="KRH39_003724"
     CDS             2057..3445
                     /locus_tag="KRH39_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG8513590.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEILVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
BASE COUNT         1459 a          341 c          608 g         1122 t
ORIGIN      
        1 aagttaccaa ttgttatgta tttacaaaac tgatatatta attacataaa atctaaaata
       61 gtttgtaata ggagtatata taataaaaat tttaattaaa taaagctagg tatatttata
      121 atattaataa atatattgct ataaaagttt gtttaataaa ttaaataaag aaaagaggag
      181 aataaaatgt acaacataga tgatatgata tataattgtc cagtagaagc tttatcaaat
      241 attttaggta aaaaatgggt ggctataatt atatggaaaa tacaagaaga taaaaaacga
      301 tttggagagc tacaaagagc tataccagac tgtagtaaga aaatgttagt tcaacaacta
      361 gattttttaa ttgaacaggg gattatagtc aaccaaaaaa agttggttaa taatgttgta
      421 gagtcaacat attttttaag tgaatcagga ttacgattgc ttcctgtcat ggaaaaaatg
      481 attatgtgga gcaataaaaa tttaaattgt gataaatact aaaatttact ctgtaagtaa
      541 tgaaaattta attaagctat gagtagaaaa atgtattaaa attacataat tttttagtta
      601 gattttaatt tagaaatggg attgtaatat tggaaataaa ataaggagga attttaattt
      661 gcaaaatgta aatttatttt tagtatttat agaaggtata gtatcattct tttcaccatg
      721 tatactgcca attttgccta tttatttgag tatattgtca aatagtagtg tagaaaattt
      781 aaaagaagga aagacgagtt ttataggaag ttctttattt aaaaatacta tattttttgc
      841 tcttggaatc tcaacaacat tctttatatt gggttcatca gtaaaagtat tgagtatgtt
      901 ttttaatgaa aataaagatt taataatgtt tattggtggg attataatta ttatcatggg
      961 attattttac atgggaataa ttaaatcttc aatattgaat agagaaaaaa gatttaatgt
     1021 taagtttaaa gaaatgaaag caataacagc ttttatatta ggttttacgt ttagttttgg
     1081 atggacacct tgtattggtc caatattggc atctgtatta gtcatggttt caagttcaag
     1141 taatcatctt agtgccaatc ttttaatagc agtatatact attggtttta ttttgccatt
     1201 cataattaca gctatgtttt acagtaaact atttaaaact atagataaga taaaatctaa
     1261 catggaaatt attaaaaaaa taggtgggat tatacttata gtatcaggaa tattaatgat
     1321 ggtaaatgga tttggaagta taagcaaaca ctttaatacg tcacaaaata gtaaaattga
     1381 aagcaagcaa gaagaaaata agcgagaaaa ttctactgat aaagaagaaa attctgatgg
     1441 taatgatagt caaaaagact ctaataatga caataatgat aaagggtcta atgatgaaga
     1501 tagaataaag tctattgatt ttactttaac agaccagtat ggtaaaacac ataaattaag
     1561 tgattatgaa ggcaaagttg tattcttaaa tttctgggct acatggtgtc ctccatgtaa
     1621 agaagaaatg ccttatatag aacaactgta taaagactat aataaaaata atgatgatgt
     1681 agtaatttta ggagtagctt cacctaattt aggtagagaa ggttcaaggg aacatgtagt
     1741 taacttctta aaagaccaag gttatacatt ccctgttgta cttgatgaag atggagcatt
     1801 agcatatcaa tatggaatta atgcttttcc aactacattt ataattgaca aggaaggata
     1861 tgtaactcag tatattccag gagctatgga taaggcaact atggcatcat ttattgaaaa
     1921 tcagagaaat aaataaaagt atttaagtat aagtttataa atcaatttaa ggttacaaaa
     1981 aagtgcctaa ttgtaccacc taaataatct tgttatagta aaagtataaa caatacaaga
     2041 aagggttgaa ttttatatga aaaaaacatt tgatgctata ataataggat ttggaaaagg
     2101 tggaaaaaca ttagcagggg atttggcaaa caggggatta aaagtagctt taatagaaaa
     2161 gtcaaacaag atgtatggtg gaacatgtgt aaatgtggca tgtataccaa caaaaagctt
     2221 agaaaattca gcaaacagcg taaaaacaaa gaatataaat tcttgggatg aagtacaagc
     2281 tgaatatgag aaagctatag ataaaaaaga aactttaatt acaaaactca gagaagctaa
     2341 ctataataaa ttaaattcaa atgagaatgt aactatattc acaggaatgg gaactttcat
     2401 tgatgaaaaa acagttcaag tcaaaactga aaatgaaatt tatgagttag tagcagataa
     2461 tatatttatt aatactggtt caagaccatt tatacctaat attaagggaa ttgaaaataa
     2521 gaatatagta tatgatagtg agtctcttat gaatctaaga gttttaccta aaaaaatgac
     2581 aattatagga gctggattta taggtttaga atttgctggt atttatagtt catttggagc
     2641 agaagttaca atattaaact ctaataatgg cattttacca aatgaagatg ttgaggattc
     2701 agaagaaata ataaaattac ttgcaaagag aaatgtaaaa attgttaata atgcaaatat
     2761 aaaagagatt aaagaagttt ctgaattagc aatagtagaa tatgaagtag atggtaaatc
     2821 taaagaatta acaagtaata tgatacttgt agctacagga agaaaagcaa acactgaagg
     2881 gctagggcta gaaaatgcag gtatagaatt aaatgaaaga ggatttatta aagtctcaga
     2941 aactcttaag actaataaag agcatatatg ggctatagga gatataaatg gaggtccaca
     3001 atttacttat atttcattgg atgattatcg catagttatt aatcagttat ttggagataa
     3061 gactagaaca actaatgata gaaagaatat cccaaattct atatttataa gtccagcatt
     3121 ttcaagagtt ggtcttaatg ttaagcaagc taaagaaaag ggatatgaaa tattagtggc
     3181 aaagatgcca gtagaagcaa ttccaagagc taaacagatt ggcaaggcag atgggtttat
     3241 aaaaatagtg atagataaaa aatccaataa gatacttgga gctagtatga tatgtgaaaa
     3301 ttcaagtgag attatccatc ttattcaatt agctgtagat ttagaagtag agtatacata
     3361 tttaagagac cgtgtttatg cacatccaac tatgacagaa gctcttaatg atattctatc
     3421 tcctaatatg ataaaagaag tataacataa gtttaatata taaacctaaa attatcatta
     3481 taagtagatt ttagaaagag gctatctcaa aaaatagaga taagtctctt
//
LOCUS       DAEORZ010000248         3486 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEORZ010000248 DAEORZ010000000
VERSION     DAEORZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.254"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..1123)
                     /locus_tag="KRH39_003725"
     CDS             complement(<1..1123)
                     /locus_tag="KRH39_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG8513591.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKVDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHK"
     gene            complement(1510..3447)
                     /gene="htpG"
                     /locus_tag="KRH39_003726"
     CDS             complement(1510..3447)
                     /gene="htpG"
                     /locus_tag="KRH39_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBG8513592.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKIKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEVVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
BASE COUNT         1088 a          626 c          342 g         1430 t
ORIGIN      
        1 acttatggta catttccttt atttgttcct tattaaaata ctttggatgt ctcttaatat
       61 ttgcaactga tattttcata caattatcag ttaaccaatc tatatctgat ttttcaatac
      121 ctaaatcact tagttttata tctaaatcta cttttttaag gaaatttcta attgcatctg
      181 aacaatcttc tgcacaagtt cctcctaata cctttgaaat attaccaaat ctttcaatat
      241 cactatccca tgaactttct ataatagtag gagtcaaagc tgcaagtcct tttccatgca
      301 ttacatctct aagaccactt acaggatgct ccattccatg tggcaaggct gtacccgctg
      361 taccaatcgt cattcctcca atagtacttg caagtgttat tttttcccat gcttctaaat
      421 cattaacatc attatatacc tttattaggt tttctgatat aagttcaatc ccataaatag
      481 cttgtacatc tgcaagaggg tttcttccat tagaaagata tgcttccata ttatgggcaa
      541 gtgcatcaaa tccaactgat gcaattatat gttttggcat tgtaaccata agttctgggt
      601 caattattga tgcttttgca tacatactat tcttctttat agatttttta tctttagttt
      661 ctgaatttgt aagtactgca aaacaatttc cttcactacc tgtacctgat gttgttggaa
      721 ctaagattat aggtaaagct tcttcacctt gtctaattcc aaatatataa tcatttaaat
      781 ctcctgggtt tttataagaa aaagctatac tcttggcagc atccataatg cttccaccac
      841 caagccctaa aacacaatca catccagttg attttataat ttctactcct gaatatatag
      901 tagttataag aggattttgt tctactctat caaaaacttc atattctatt ttagagtctt
      961 ttagtaaatc tattgtttta tcaagtaatc cagttttctt tgtactattt ttacctgtta
     1021 ctatcaatac ttttttgcca tatttagata cttcttttcc cacatcatta attctgcctc
     1081 tcccaaataa gatatttact ggcaaattat aattaaaatt catgctaaaa cctcctattt
     1141 ctactttagt aataatttta tgtttttaac aaagtgtaat tatgtattta aacataaact
     1201 aaaaaacaat cttttactta attatatgta ttatcttatt atatatcaag ttaactatat
     1261 ttaactaaat agagtttttt attagaacct acttaaatat ttaaaattaa ccaaagataa
     1321 aaaaataaag aacggtctgt ctcaaaatag agataaatat atttttcatt gttgaaatga
     1381 ttaaaataaa tttaatttca cataaaaaaa gcgcctcatg aactgaaaag ttcattttga
     1441 gacacttctt tcttatcact gatattgaat actttttata aatttgcttc aatgtattca
     1501 aattcaatct tataattcta tcaaaagact cataagctta ttacttcttt gtacaaatga
     1561 atctaactca tctggcttta attctttatt agaaagtaaa gctaaatctg ctatctggtt
     1621 acaaataagc gtaatcttat ctttcttttc ctcatcatct ttaagagaaa ccaatttttt
     1681 gattattgga ctgttctcat ttattacaag agttttttct tcttcaaaat tcattcctaa
     1741 atccattcca gcaaatcttg actgcatttc tgccattcta atagattgtt ctgatactaa
     1801 caccatagct ggagtgtctt catttttcaa tccttctaca gaataattct tcactctttc
     1861 tccaacaact tctttaaaca gaccttctat cttagctatc tcttctttgt tttcttcact
     1921 atctttattt tcatttttat cttttaatac atcagaaagg tctgcatcta ttctattaaa
     1981 cttaactcca ttctctttat attccataaa tgaaataaaa tgagtatcta ttgatgagtt
     2041 taaaacaaca gcacttaagt cgtattcttt aaatagttta atgtattgag attgctgttc
     2101 tttatcactt acataaaata ccttattttc atgtttgtcc ttacattttt ctaaataatc
     2161 ttgtaatgtt atatactcat catcaatagt tttgaataat atacattctt ttatcttctc
     2221 atagaaactt tcatctttta agcaaccata tttaataaat atttgtatat catcccaaaa
     2281 cttattgtat tcttctcttt catttttaca aagagatttt aacttatcag ctactttttt
     2341 aattatatgc ttagaaattt tgcttacatc cctgtcattt tgtaagaaac ttcttgatac
     2401 atttaatggt aagtctgggc agtctataac accttttaat aaaagtaaaa attctggtat
     2461 aacttctttt atattgtcag ccacaaacac ttgattgttg tataatttta cttttccttc
     2521 agtcagttca aactcatttt ttaatttagg gaagtaaagt atacccttta aattgaaagg
     2581 ataatctaca tttaaatgta tccaaaataa aggttcatca aatacattga atacctttct
     2641 ataaaactct ttatactcct catcagtaca gtcttttgga gattttaacc atagaggatt
     2701 agtgtcgttt aaaggctcaa ttacttttgc atctacaatc tcttcttttc catcttcctt
     2761 ttgtttttta gctttttctt ctgcttcttt agcttctctt tctaatcttt caacatcttc
     2821 taaatatatt tctactggta agaaagaaca gtatttattt attattcctc ttacagtaaa
     2881 ttcatctaaa aattctttac tatcatcatc aataaatagt gttatagtag ttcctctatc
     2941 atttctagca tctgattcag atatttcgta ttctgtacca ccttcagata tccatcttac
     3001 aggagtcgct ccttctgtat atgaaagtgt atctatttgt acctttttag acaccataaa
     3061 tgcagaataa aatcctaatc caaaatgacc tattatatca tttgactctt ccattttatc
     3121 cttatattta ttaaagaaat cttcagcacc tgaaaaagca acttgattta tgtatttttt
     3181 tatctcttct tcagtcatac caattccatt gtcaataaat ttaagtgtac cttctccttt
     3241 atttacagat actgtaattt tataatctga tgacttattt tctgatattt ctcctagtga
     3301 tacaagtctt ttatgtttac ttactgcatc acatccatta cttataagct ctcttataaa
     3361 tatgtcttta tcagagtata accatttttt aattatagga aaaatatttt ctgtatgaat
     3421 tgaaatactc cctttttcaa attccatttt ttatccctcc tcattttata attaatattt
     3481 taaatt
//
LOCUS       DAEORZ010000249         3460 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEORZ010000249 DAEORZ010000000
VERSION     DAEORZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.255"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(204..1475)
                     /gene="serS"
                     /locus_tag="KRH39_003727"
     CDS             complement(204..1475)
                     /gene="serS"
                     /locus_tag="KRH39_003727"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG8513593.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(1601..1806)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1934..2815)
                     /locus_tag="KRH39_003728"
     CDS             complement(1934..2815)
                     /locus_tag="KRH39_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG8513594.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(2827..3336)
                     /locus_tag="KRH39_003729"
     CDS             complement(2827..3336)
                     /locus_tag="KRH39_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBG8513595.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
BASE COUNT         1121 a          594 c          330 g         1415 t
ORIGIN      
        1 ttctaaaata atttttagat attcatacaa gagtgtttta taaaaatatt tagtgtttta
       61 tgatgaaaat tctgctgtgt aaattaacat aaaaaaacta tgaaattaat atttataata
      121 atttcatagt ttttttattt attgtttact cataatatat tctcttcatc aaagcatact
      181 ctacatatac aattaatata aatttatttt ataacatcaa cacccatata aggtcttaat
      241 tcttctggaa ctactacaga accatctgcc tgctgataat tttctaatat agcagccaaa
      301 catcttccta tcgctaatcc agaaccattt aatgtatgaa catattctgc ttttgatttt
      361 ttatcccttt taaatcttat tcctgctctt cttgcttgga aatcttcaaa atttgaacaa
      421 gaagatattt ctacatatct attgtaactt ggcatccaaa cttctaaatc atatttaaat
      481 gcagctgtaa atcccaaatc tcctgtacat atttttacaa ctctatatgg aagacctaac
      541 atttgtaaca tagtttcagc atcatgagtt aatgattcta attcattata agaatcttct
      601 ggtttaacaa atttaactaa ttcaacttta ttaaattgat gttgtctaac taaacctctt
      661 gtatctcttc ctgctgaacc tgcttcagat ctaaaacaag gagtatatgc acagtattta
      721 attggaagct ctgcaacatc taatatttca ttagcatgta tattagtaac aggaacttca
      781 gctgttggta ttaagaaata ctctaatcct tctattttaa acatatcttc ttcaaattta
      841 ggaagttgac cagtccctat aaaactattt ctattggcca taaatggagg taagacttcc
      901 gtatagccat gcttagctgt atgagtattt aaaaagaaat tcattaaaga tctttctaat
      961 ctagctccta gtcccctata taaagtaaac cttgacccag ttattttccc tgctctttca
     1021 aaatctaaaa tacctagacc tgtacctata tcccaatgcg ctttatgctc aaaatcaaat
     1081 ttagtaggtt ctccccaagt tcttatttga acattgtctt catctgtttc accttgtggt
     1141 acctctggat gaggaacatt tggtattctc attaatgtat attccatttt tgcttctact
     1201 tcttttactt tctcatcaat accttttatc ttatctgata actctttcaa tcttgccttt
     1261 tcttctgtta catcttttcc ttcttttatt aattgaggta tttttttaga atttacattt
     1321 aactcatttt tcataacttc aacttcttga agtatttctc ttcttttatt atctaattcc
     1381 actacagcat caaggtcaaa ttctctttca cctcttcttt ccatagcttt tttgatatca
     1441 tctagatttt ctcttattct tttaatatct aacatattaa ttcctcctaa taattttgat
     1501 aatataagtt ataattccaa cttaatttac aaacattata cttgtaaact cttagtttaa
     1561 acaaatttta aaaataaaaa aagacctccg tttaccctat aaaagggacg aaaatctttt
     1621 atccgcgttg ccaccctaat tgactatact aaaatgtata atcctctcag acctataacg
     1681 taggtaaacg tctagcttta actagaagct ctagagtaga ttcaaaaacc attgtaatga
     1741 gttttcacca tccactcatt ctctgaatac aatctgtttt ttactatttt ccttcacagc
     1801 tttttaacta attgttttat ttgataatca ttatattata tatattaaca tctttcaata
     1861 ctattattca aattttttta gaattttctt taatttttta tattaaattc attttttcta
     1921 tttaattaag accttatctt ttatccattg tatttagatt acctgtatta ttaaataata
     1981 atgaagaagg aacaaatgga acttttatat cattctcatc aaatttattt tttacctttt
     2041 ccattacatc aaaatataca tcccagtaat catcagtttt tacccaaggt ctagcaacta
     2101 tttgcatggt gttatttcct atagagttta aacctattat aggctttgga tcatttaata
     2161 ttcttttttc gtttgtaaaa atttcattta atatagactt aactacatct atatcactat
     2221 catatgaaat attatatgaa aaatctattc ttcttttgtt ttgatgtgtg taattaatta
     2281 tattattact tgtcagttga aaattaggta taattatagt tttattatct ggtgtcttaa
     2341 gaacagtgct aaatatttgt atgtcagaaa ctgtaccttc tacacctgaa ccttgaatat
     2401 aatcacctat attaaatggc ttaaaaaata aaataatcat accagagcct aaatttgata
     2461 atatttcttt aaaagctaaa cctatactaa atccagcagc tcctaacaca gctacaactg
     2521 atgtagtttg tatccctata atacctaagc ttatcaatga tataatagat aacatagata
     2581 tatatattcc atatacaata aaattattta cacctttact cattccatat ttgattagaa
     2641 actttgaaac catccttctt actattttag atatataaat acctatcaca aatacaatta
     2701 ttgcatatat aatatttggt atattatcac taaaattatc gagcatattt tctaaagttc
     2761 tcttagttat attgctattt gttgaggcag tcttagccaa tgataaaaaa ttcattcaac
     2821 tcctccttat gataatattc cgttgtcctt taactctttt actatagctt ctatagtttc
     2881 ttcttttatt gcctcaattg tatgaatatg gctacattct gtcagaaccg ataattgttt
     2941 aaattcattt gtctcaactt tcttcataaa gttgtctata tccattttag aataaatatt
     3001 aagttctgca gttatctgac catatgttgg atgatttaca atgacatctt ttataatacc
     3061 acctaaatct acaattatct ttaattcatc ataaatttct ttagaatcct tatgattttt
     3121 acactttatt tgttttgtaa aatacggttg attatatatt atatatcctt gaggtgtagc
     3181 tattatttca aatcctctag ctcttataag ggctatatct tgaactatta cttgcctact
     3241 tacatttaat agttcagcta gtgttccacc ttttacagga tgcttagatt cttttaaaat
     3301 atctattaat ttacttcttc tttcattaga attcatatta caccttcttt ccaaataaaa
     3361 actttaccta tactatataa ttttatattt tatcttatta taaatcaata ttgactttat
     3421 attctattta atcttaactt ttataataaa taaaaaaaga
//
LOCUS       DAEORZ010000250         3362 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEORZ010000250 DAEORZ010000000
VERSION     DAEORZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.256"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            138..755
                     /locus_tag="KRH39_003730"
     CDS             138..755
                     /locus_tag="KRH39_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBG8513596.1"
                     /translation="MEIFAIGHSNYPYDKLIDMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFVYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(922..1374)
                     /locus_tag="KRH39_003731"
     CDS             complement(922..1374)
                     /locus_tag="KRH39_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBG8513597.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            1822..3180
                     /locus_tag="KRH39_003732"
     CDS             1822..3180
                     /locus_tag="KRH39_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8513598.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDNEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAMSIAIARIIGCIIIIRAL
                     FKGNEFISMSKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFVV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
BASE COUNT         1319 a          332 c          578 g         1133 t
ORIGIN      
        1 taaggatata aagtaaaaaa atataaagta taaagaaaaa agatattgga tatttaaagt
       61 aatactttat ttaattttaa gtaaatcggg tatctacata tatagacact aatttcaaag
      121 aatgggaggg tatttatatg gaaatattcg caataggaca ttcaaattat ccatatgata
      181 agcttattga tatgataaaa aaatatgaca taaattgtgt agttgacatt agagaaactc
      241 catattctaa atataatatt caatacaata aagaggcatt taacgaatct cttagaaatt
      301 ccggatttgt atatatatac atgggaaaag aatttggagc taaaagaact aataaagatg
      361 tatatacgca agaaggttat gcagattttg agaaagtagc aaaagaagat atttttttaa
      421 acggaataga aagattaaaa aagggttgtc aaatgggtta taggatagtt ttattaggag
      481 ctatgcaaga acctataaga tgccatagaa gtattttggt gggaaaagta ttaaataaag
      541 aaggttttga tgtaaaatat ataatgcatg aaggaaattt ggcatatcag gaagatatag
      601 aggaatcgtt attggataag tattttagtg atagaaagca actgagtatt gacaatttat
      661 tagggagtgc attaacgaga gaagaaatga tacaagaagg ttataagcta gctaataaag
      721 aaataggata tagaactgag aaactaggta aataaaaata taaaagtaga taaaatacag
      781 tacttacctt aagtaaaata atattatgaa tttataatag ataggcatga tgtgccaatt
      841 aaaaatattc tagttattta ataatatcat taaatatcat taaatttagg gtatttttta
      901 atagcacatc atgttgcagt tttaaaaaat aaaatctcta atattgccat aatcaactgt
      961 tctattaaga gtaggatgac tagatataaa tctaactgta atttttgaat ttaacaaaca
     1021 aggatttgaa attaaaaata ctctatctcc attgacaata gaagtaatgg ctactggcaa
     1081 aatatctaca aatgtatcat atctgttttt agcaatttga taaggataat ttcctacaaa
     1141 aacaaataga ttcaaatcat atatattatc agtaaaccaa gcccactggc cataaacttt
     1201 gtcgatattc attgtgccta aaccaagtgt atcaaagtat gttgctatag ttaaataaat
     1261 attttccatg ttttctaaat atgaaacagt atatcttctg aaacgtatgg gtgctgtgga
     1321 agaaatagga taaaccatat ttacatttag attatttgca tttaaattaa ccaaagtatc
     1381 acatcctaaa attagtctat aaaatacaat atttaaatgg atgcgatttt gttacatcta
     1441 aagtgtaata ttttatatat tgaaaaagtg gtaaaagttt gaatgattaa ataatattat
     1501 aaaaaattta taaaatgtac ttattgttat tgctacacga tagtgataca atattaacga
     1561 aatatagcta cttttaataa taactgaaaa tatttatatt aaattaataa atattttaaa
     1621 tgtggtatcc ataaaaaata ataaacataa gataattata aagatatact gtgttcaagt
     1681 gatattaaac aagatttata ttatttgaaa gcagtatatt ataatgcgaa caatacaatt
     1741 ctaaactttg cattgtcaat ttacagttcg ataaactaat taaaaattaa atttatataa
     1801 agagggaaaa gaggaatagg gatgtttata agaaaaaatg ttataaagtt agcaattcct
     1861 ataatgatag agcagacgtt tgtcatgctt ttaggagtat ttaatacaat gatggcaggt
     1921 catattgggg aagaagctgt atctgcagtt ggaatggttg attcaataaa caatatattt
     1981 atatctgtat ttgctgcatt atctgttgga gctactgttg ttgtagctca acaaatagga
     2041 aaaaaagata atgaaaaggt gaatgaaact attaagcagg ctctagtgtc tggagtaata
     2101 ttatccttta caataacaat attgatgtgg gtttttagaa ctggtgtaat taatttattt
     2161 tatggttcag cagaagaatt ggtaaaatca aatgcaaaat tatacataga gtttacatta
     2221 tttacgtatc catttattgc gacacaacaa atagcaaatg gtattttaag agggtgtgga
     2281 gatgtaaaga aacctatgta tgtgactatg tttatgaata tagtaaatgt aacattagga
     2341 tatatactta tatatggtat tgatttaaat cctctaggaa taaatttgca aacaccatcc
     2401 tatggcataa caggtgcagc tatgtctatt gctatagcta gaataattgg atgtataata
     2461 attataagag ctttatttaa aggaaacgaa tttatatcta tgagtaaaat atttccattt
     2521 aagattgatg ttgaaactca aaagtctata tttaatatag ggataccagc tggagttgaa
     2581 caactacttt ttaatgcagg taaattgatt gttcaagtat ttatagttac tatgggaaca
     2641 tcagcaattg catccaatgc aataggaatg tcaattgcat ccataataaa tgtaattggg
     2701 aatgctttag ctttgtctgc tacaaccttg gtgggacagt atataggacg tggagatatt
     2761 aaaggagcta agagtacttt attatattta actaaatttg caacaatttg tttatttgta
     2821 gtaggagcta tatttatacc aattgcaccc tggatttcaa gtatgtatac tcaaaatgct
     2881 agtgtaattg acatctcaac acaattaatt cgaagtgatt gtttagctat ggtaatatgg
     2941 cctatatcat ttgtattatc agcagggcta aagggagctg gtgatactag atatacaatg
     3001 atgactgcag taataggtat gtggattttt agaattttta caggttacgt attgggggtt
     3061 gtattagaat ttggagtgtt aggaatttgg ataggtatgt atactgattg gcttgttaga
     3121 gggactttat attgtttaag attaagagga acaaagtggc taaaacacaa agttgcatag
     3181 aatatatgaa atcactttta gatggataac tctatttaga agtgattttt tatgtagtta
     3241 ttatcataat tataagtatg atatataagg tgattaaatt acataaataa agcctataat
     3301 agtatggatg ctataaatta tatattgagc agaattacat aatttggcga taaatattag
     3361 aa
//
LOCUS       DAEORZ010000251         3330 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEORZ010000251 DAEORZ010000000
VERSION     DAEORZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.257"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..964
                     /locus_tag="KRH39_003733"
     CDS             <1..964
                     /locus_tag="KRH39_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG8513599.1"
                     /translation="NQYKGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESI
                     NSDIMSIDNNSKEKAIKLIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYT
                     WQNAILNELIIDVANPLDVNVLNIGSNFVHLEGYQQNWGEIIDFVQSKFDGLVTYRTN
                     WWYTKKDDLSSKLFYEQKLNNNFFDKLDFISIAAYFELSNKSVNTVDELISALHSSTV
                     NNRGQNIKQEIYNLYKAHRKPIFFGELGFSNRESASSQPWNHAPSKVVNGEEQARCFE
                     AYKQVFENEDWINGFSVFCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            1018..2142
                     /gene="wecB"
                     /locus_tag="KRH39_003734"
     CDS             1018..2142
                     /gene="wecB"
                     /locus_tag="KRH39_003734"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBG8513600.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLAKKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPKKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDVIIKKFNCKYLN"
     gene            2218..3192
                     /locus_tag="KRH39_003735"
     CDS             2218..3192
                     /locus_tag="KRH39_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG8513601.1"
                     /translation="MARKLIKHLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKQLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         1394 a          327 c          583 g         1026 t
ORIGIN      
        1 taatcaatat aaaggtaaga ttaaatcggc caatttatct gtagattatg aaataaatca
       61 agttatgaag gatatagata aattgggatt gaatacagta aatgtaccta taattataaa
      121 tgtagagtcc attaattctg atattatgtc aatagataat aatagtaaag aaaaggcaat
      181 taagttaata aaaaaattaa ataaaaaagg tatttcaaca atattagagg catatccatg
      241 gattgaaaat ggaaaattgt atgagactga ctggaaccct ataaataaaa aaagattttt
      301 ttatacttgg caaaatgcta tattaaatga acttataatt gatgtagcaa atccactgga
      361 tgtaaatgtt ttaaatattg gttctaattt tgtacatttg gaaggatatc aacaaaattg
      421 gggtgaaata atagattttg tacaatccaa atttgatggt cttgtaacat acagaactaa
      481 ttggtggtat acaaaaaaag atgatttaag tagtaagttg ttttatgaac aaaagttgaa
      541 caataatttt tttgataagt tagattttat atctatagca gcatattttg agttaagtaa
      601 taaatctgtt aatacagtag atgaattaat ttctgcctta cactcatcta ctgtaaacaa
      661 tagaggacaa aatataaaac aagaaatata taacctttat aaagctcata gaaaacctat
      721 attttttgga gaattaggtt tttcaaatag agaaagtgca agttctcagc cgtggaatca
      781 tgctccttca aaagttgtaa atggcgaaga acaagcaagg tgttttgagg catataagca
      841 agtttttgaa aatgaagact ggataaatgg tttttctgtg ttttgtgtag gtaaaataga
      901 tgacgaaaaa aatttttacc catcaaaaga gagtataaaa gttattaagt cttggtatga
      961 ataattttga aagtgtagaa tttatataga ttaaatagac gattaggaga gatataaatg
     1021 aatgacatca aggttatgac tgtatttgga actagacctg aagctatcaa agtagcacct
     1081 ttaataaagg aacttgaaaa aagagagaat ataaaaagta ttgtttgcgt tacagctcaa
     1141 catagagaaa tgcttgacca agttatagaa acctttaata taaatgtaga ctatgatttg
     1201 gatataatgg aaaaaggaca aagcctgaat gatataacat gtaagatttt aaataaactt
     1261 cccttaattt taaacaagga aaatccaaat ataatattgg tacatggaga tacaaccaca
     1321 actctagcta caagtttgac tgcattttat aataaaactc ttgttggtca tatagaagct
     1381 gggcttagaa cctatgataa atattctcct tttccagaag agttaaatag acaacttaca
     1441 ggtattattg ctgacatgca ctttgctcct actaatttgg ctaaaaaaaa cttgatttct
     1501 gaaggaaaac ctaataataa tatatttgta actggtaata ctgctattga tgctttaaag
     1561 atgacaataa aagaaaacta taatcatcct ataattgatg agattggcaa tgataggatg
     1621 attttgttaa cctctcacag aagagaaaat ttgggtaaac ctatgaaaaa tatattcaga
     1681 gctataaagc gaatagtaga tgattttgaa gatgtacaga tagtatatcc catacattta
     1741 aatcctaaaa tacgaactat agctgatgag atatttggaa aatttcctaa aaaaattcat
     1801 attatagagc ctttagatgt agctgatttt cataactttt taaataaatc ttatatgata
     1861 atgactgata gtggaggaat acaggaagaa gctccatcat taggtaaacc agttcttgtg
     1921 ttgcgtgata aaacagaaag aacagaagga attgaagcaa agacacttaa attagtaggt
     1981 actaatgaag atagaatata taattcagtg agtgatttat taataaataa agataactat
     2041 gttcagatga gtaaagcatc taatccatat ggagatggaa atgcaagcaa atatatagta
     2101 gatgtaataa ttaaaaaatt taattgtaaa tatttaaatt agataaggtc taaaacatta
     2161 actaatgata taagatttat gataatataa ggaatacaat ttttaagggg gagtaatatg
     2221 gctagaaaat taataaaaca tttaggtaaa agtaaaagtg taaagagagt gaaactttta
     2281 tttaagaaga tttttattac tgtattcata gtagcaagta tagttgctat tttcaatata
     2341 actaaatatt ttgaggaatt atacaaagta agggatttaa aaagtactaa aattgaatac
     2401 tatatggatg tggctgatga agcaggggat ggaaaggtcc aattaagttg gaaggcctta
     2461 cttgctatag acatggtaat acatgatgaa gatttaagta atataaaaaa gaaagacaca
     2521 ttggatatag gggaaaagtt tatagtagaa gataaaaatg ataaaggcga aaaggtgtat
     2581 aaggtaaaaa agtttaataa ggtattaagc gaattgaaat ttgactcttc tcaaaaaagt
     2641 agagcaagaa aatacatgaa agatttggaa tacacatacc ttggaaataa acaattagat
     2701 agtagtgatg aaaaaattaa atttataaag cagttagaag actcagctat aagagaatat
     2761 attgattatg gaatattgcc atctataaca attggacaag ctatattaga gtctggctgg
     2821 ggaaattcta aacttacaaa acagagtaat aatttatttg gtataaaagc agataaagca
     2881 tggaaaggaa aaagtgtaga aatttcaact tcagagcatt ataatgaaaa aattgtagct
     2941 agttttaggt cttataattc attacaagat tctgtcaaag accacagttt atttttaatt
     3001 aataataaaa gatatagaaa acatgggttg tttgaggcaa aagattatat tagtcaggct
     3061 caagcactag aaaatgcagg atatagtaca gcagaagata aaaaaggaaa tcgtatatat
     3121 gcagagctat taatagatgt tattaggagt tataatttgc aattgataga caataaagtt
     3181 gagacaaaat aattgttaac tttatatgag tagataacaa gataagatta ttttctacaa
     3241 aaagaataga aaaaatgtaa aaaaaactaa aaaaatgtat tgacttgtta tttaaaaggt
     3301 gatattatta ttactgtact cgaaagagaa
//
LOCUS       DAEORZ010000252         3307 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEORZ010000252 DAEORZ010000000
VERSION     DAEORZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.258"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(12..2093)
                     /locus_tag="KRH39_003736"
     CDS             complement(12..2093)
                     /locus_tag="KRH39_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBG8513602.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYVIKGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKMGNSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDNNIIYIGKPENTAEEILDILSTKEQTLLSSNCIMKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVFVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLVLSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSD
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIVVIIL
                     SMLYIKKYKRR"
     gene            complement(2116..>3307)
                     /locus_tag="KRH39_003737"
     CDS             complement(2116..>3307)
                     /locus_tag="KRH39_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG8513603.1"
                     /translation="LINIILAMGGYIFYFKNFDKEIKEIDEYPMISILVPAHNEAKVI
                     GRTVESLLLLNYPKSKMELIVINDNSSDNSKEILENIKDRYNNYNFTIINTDSLTGGK
                     GKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLVQTIVMNDELGAVIGKFRTRNKN
                     KNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTNFILRRSIIEEIGGWDSKAIAED
                     TEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQRTRWAKGNIYVLMKYIKNIFKQ
                     GRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISKLVEISIPINFFLIWILSYLLFI
                     IEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFVAIKGMIEYLKDIIFKREVKWYK
                     TERF"
BASE COUNT         1094 a          519 c          310 g         1384 t
ORIGIN      
        1 aatttatcat attatcttct tttatatttt tttatatata gcattgataa tattattact
       61 acaattatag taataaaaga aatcattaaa aatactttag ttgtcttatt tatactcata
      121 ttccagcttg aatcttttac ttctttctta gattttaaat tataagctaa atcttctacc
      181 tctccgtatt catcaattat caatgtatct ccttttaaat cattaacttt acttttattc
      241 aacaaataat ccattcctaa atataagttt ttttcattca ttgaactaat taccatgata
      301 tttttttgat tattatatgg tgattttatt aattgaatgg tagaaatatt ttttccataa
      361 tcatcaataa agcttatctt atcatttgat ataaaatcgg agtaattttt atcgaacttt
      421 atatttaaat tattatttaa catctttata acagaattat tatgtggcac tccaaaaact
      481 acaatattag tatccttata cttgtcacta aattcttttc ctctaattac atttatattt
      541 ccattatgtg aatttatatt cgcacctaaa gtaacaccta atctaaacat ccaggtcata
      601 gcttgtgacc ctgaataatc tggcattatc acagttaaat cattaaattc atcatctcta
      661 acaaatggat acggataatt ttcaaaagat aaggtttcat tttctttcgt agataacacc
      721 aaataagaat tatttgatac atatgcccaa ggattattac tctctctagt aacacaattc
      781 gaatttttta tactcaaatt aaatgtcaat ttaacttgat aatagttttt attatctata
      841 tctttaggta tttttaattc taacttatca ttattagaat gtgacctatc caatttttta
      901 cttcccacaa taacatcatt tatagacact gtaactaaag atttttcaaa atctaaattg
      961 tcagagtatc ttaaatttaa gataatcttt gaaccatcat ctaacacctt acccgttggt
     1021 attttgacat caaacaaagc ttgttgatta aatgctcctt ccaacaaaaa gtcagaatat
     1081 cctaagtctt ttaatgttaa atgacctaaa tttaattttt gttccctatt gatttttata
     1141 ttagtctctt tgtttacaaa tactgatgat gataaaactt gattagaaag tctattttct
     1201 attaataaat ttgatgcttt tattaaatca tcttcattac taccaattac caccatcatc
     1261 ttcttattct tattgtatgg tgaatctact tgcttcatta tacagtttga agataatagg
     1321 gtttgttcct ttgtacttaa aatatccaaa atttcttctg ctgtattttc aggtttacca
     1381 atatatataa tattattatc tgaccaattt ttcatttctg aatataattt aacatctaac
     1441 tttaaatcat cattttttgt tatttttcca aatgctgatg ccaaattaaa cactgctgta
     1501 gtctctcctc tagtcatatt atctggtact actatagttg tatctaattt atgattattt
     1561 tcaatttctg catatggata cggatattca tttattgaat ttcccatctt tttctgttta
     1621 tatctaattg atatatatga ttctttgtga ataaccatcc agttcccagt attggaatca
     1681 tcttggcaaa ttttatcaga tattgttttg tatgttttta tttttatttc gttatatcct
     1741 ttaattacat aatcttttgg aactaatact tctagtgtat ttttataatt tgtcttagca
     1801 tttaatttaa tagattttat tggtacatta tttatcaaaa cagttaaact tgaaacatct
     1861 ccatttaata tttgactctt actaaaatta agatgtaata aaacttcttc aatatcccaa
     1921 tttttattta cttcaaaaaa agtactatta cttcctataa caccatcaat agttatatct
     1981 ctttcaaact tataattttt aactttagtc tcgtcagcat ttactttgta tataagtgat
     2041 atatttgaaa aaaacaatac taaagatata attgatataa taaatttttt catgttaatt
     2101 cctcctgtac atgaatcaaa atctttctgt tttataccat ttaacctctc ttttaaaaat
     2161 tatatctttt agatactcta tcattccttt tatagctaca aacagccata gttgagagta
     2221 tgtaaaatac attattgcaa ctatgaatat attttcaatt gttgcttcac ctttttctat
     2281 agtaaggctt atactcactt ctataataaa taaaagataa gatagtatcc atattagaaa
     2341 aaagttaatt ggaatagaaa tttctactag tttagatata cttaaaacaa ataaaatatc
     2401 ggatataatg acagatgtta aaaatagaaa atatacagaa aaaaagtaag ctatatcaaa
     2461 tacaatctta tttcttccct gtttaaatat attttttata tatttcatta atacatatat
     2521 attccctttt gcccatctag tacgttgctt tatccacacc ttaacagttt ctggctcttg
     2581 ttcccatgtt atagattgtg gtacaagctt tatcttgtat cccaatttat atatcctaaa
     2641 acttatttct gtatcttctg caattgcttt actatcccat cctccaattt cttctatgat
     2701 acttctcctc aatataaagt tagttcctgg tattgtacat agattaaaaa gttgccatct
     2761 accagcttgt gacatccatt gaaagcttag tgtttctatg tttataaatt tggttaatag
     2821 atttttattt ttatttcgag tcctaaattt cccaataaca gcacctaatt catcattcat
     2881 tactatagtt tgtactaaat atctaagtgc atttttatct ggtgtgttat ctgcatcata
     2941 aacagctata aaatctcctt ttgaaatcgt atatccaata tttaatgcgt tagattttcc
     3001 ttttccaccc gttaaactat ctgtatttat tatagtgaaa ttataattgt tgtacctatc
     3061 ctttatattt tctaaaattt ctttactatt atcagaagaa ttgtcattta taacaataag
     3121 ttccatctta ctcttaggat aattgagtaa tagcaaagac tccacagttc tacctatgac
     3181 tttagcttca ttatgagcag gaacaagtat tgatatcata ggatattcat caatttcttt
     3241 tatttcttta tcaaagtttt taaaataaaa tatatatccg cccatagcta aaataatatt
     3301 tataagt
//
LOCUS       DAEORZ010000253         3282 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEORZ010000253 DAEORZ010000000
VERSION     DAEORZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.259"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..366)
                     /locus_tag="KRH39_003738"
     CDS             complement(<1..366)
                     /locus_tag="KRH39_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG8513604.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFD"
     gene            complement(387..1466)
                     /locus_tag="KRH39_003739"
     CDS             complement(387..1466)
                     /locus_tag="KRH39_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBG8513605.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            complement(1520..2809)
                     /locus_tag="KRH39_003740"
     CDS             complement(1520..2809)
                     /locus_tag="KRH39_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG8513606.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     misc_binding    complement(2972..3183)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1086 a          659 c          359 g         1178 t
ORIGIN      
        1 atcaaactca tatccaccag ataccaatat tgaatctaat tctatttttt ctcttaagtc
       61 ttctggccaa tctataaatg gaagaatggc agtagtggct ggctttccta ttcttatcat
      121 agccacatta ttaagtcttt ttctttctac aatagcattt gtacaatgag ttgtaccaag
      181 catagcatat tttattaatg aatggtcaat cttactttca tctataactt tttttaaggc
      241 attgtaaaca ccagtttcaa catcttttgt agttgccacc ttaactgaat gaattacttt
      301 taattcccca tccagtatac aagcatctgt atttgttcct ccaacgtcaa ttcctacacg
      361 atacatcaaa atttcctcct aaacttttat tttccttgta attcttctac tgttttatat
      421 tccacatcat atccaaagta tccaggtcca actgtctcaa ttcctttttc tgttctccat
      481 ttttcattac aaggaaaagc tactaccata acacgagagc catattttat tctctctgta
      541 gttattggaa atccagtttc taaatctaat actgctatta aatctggtgt aatacaaagt
      601 ggtgtatcac cttttttggc aattaaattc tcattttgga ataatataga atactctcca
      661 tcatactcat taatcccagc aaaaaaggca tgtcctctag taaatccacc tttcaactct
      721 ctcttaatat caattacttt tccttcaaat aacttgtatc cacgcaattc ttttagtata
      781 ttctcaacag caccttttga atcagattta ttttcaagta aaaaccctcc tatagtttca
      841 gctattgtaa gagtattacc tatagcagct tttttaactt gagctccact taaattatag
      901 tcactaatag aagaacttcc tcccatttct acagttaaag ttctagctaa tctttctgac
      961 caaattccat ccacaggata aaggatggca gaattacctt tttcatcaga cattacggca
     1021 ggagatgcct catatccttc aagataaaaa gttaccattt gagcttcagg gaaagctcta
     1081 cccatagaat ctgcatctaa gataggaagt ccttttttag cagcaactgc tataggcata
     1141 agtgagttta tccctcctat ttctatagga attataacat ccacttttct atttaataat
     1201 ttctccatta tttctaaagg ggtagttaac tcaacttctg aaagtaattt ttctattgta
     1261 attacaggag aacccatacc agatactgga actaccaaag catcatcatc aagttcatct
     1321 aaacttatta cttttacagg accatacttt ttaatggctt gttttgcaac caatttacct
     1381 acatatgggt cacctccacc tccagttcca agcactgctg caccaactgc tattttatct
     1441 acactttctt ctgttaaaaa tctcatgtta ctttctcctc atatttctaa tttattttat
     1501 actttataaa caaatttctt tatactgctt tgtctatttc ttgcttattt tttgttgaac
     1561 aaagcacttt tccaataata gattgtacta caaaccctgc tataaaacca tctaaaggtg
     1621 ctatagtagt aagagttata aatccatatg ttgaaaggta agttattaat ataccaatta
     1681 tccaagacac tattgaacgt ttaactattg gtttatataa tactccttta tcaaagcctt
     1741 taagttcttg ctttactatg taatattctg ctgtatatac accaccaaca ggtgcaatta
     1801 caattccaag taaatttaaa aatcctataa aaccttcata tattccaaat acagccaata
     1861 atgttgctat aataccaagg actatagtta atactttctc aggagccttc ttaagtacaa
     1921 gagctatact taatgatgct gaatatacat tacttgtatt tgtagtccat tgtgctaatg
     1981 taagtactat tatacctggt atagcaattc ctaatgtaat aaaaattctc ataatatctg
     2041 aagttcccat acattttact aatactatcg aaactattat cataaaagca tttccaaatt
     2101 gtattcccaa aaagcttgaa agagcacagt ctcttctact tttagcccaa cgacttatat
     2161 caggagatac aattgcacca actattatta tactcattga cagagaaact ccacctgcaa
     2221 atgtcatagt agatttcata ttatcatcaa ctgaaattcc atttgctcta aaagctaaga
     2281 atatagttag catcataaga actaataaaa atggtactga atacacactc aacttctcca
     2341 ttgccttata tccataaatt gcagtactca tcatcaagag accacctatt aggcttaaaa
     2401 tctgcatact tacatctaaa ttaaatatat cttttattat tatctgtgca ttttgagcaa
     2461 agaaacctac ttgaactcca aaccaaccta atagagaaac ccctataact agagaaaata
     2521 tttttgctcc agcttctcca aatacatact ttgttatcat agatgtagat aaacttgtag
     2581 atgagccaac caaagagcat gtagtataca aaattgcaga aaaaaatgaa ccaacaatta
     2641 ctgacaatat tgcttgtttc attggcatac tctgtgccaa ctcagcgcct aaaataacag
     2701 atgataaatc cacacctatt gctactaaaa ctgaaaacat agctacccaa ccacgttttt
     2761 gattctctgg aatctgttcc agagcatagt taattttttc ttcttccatt ttcatccctc
     2821 catttaaatc gtaaaactac tgaccttagc atattttaca attttctaat ttataaattt
     2881 attaactatc atatttttat aattttcatt taatatatag ttttaaattt tctgaaataa
     2941 gtttataata aaaaacgcct cttggaaaaa tccaagagac gaatataaat ccgtgttacc
     3001 actcttatta gtaaaaatta attttactca ctcattttct taaggcgaat taaaccacta
     3061 ttctaacata attacaatag tattctccaa agtccttttc acataaattc caatattagt
     3121 tctcagccaa atctaactct ctgtaaaatt cctttatgct actttcttct tctacgaata
     3181 tataatattg atttgtagtt aatttatgat ttatatcata atatatatgc aatattttgt
     3241 caacttaaaa agtcaattac ttgcattttt tagttttata tg
//
LOCUS       DAEORZ010000254         3240 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEORZ010000254 DAEORZ010000000
VERSION     DAEORZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.260"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..2067
                     /locus_tag="KRH39_003741"
     CDS             <1..2067
                     /locus_tag="KRH39_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBG8513607.1"
                     /translation="LYNDTTSISVMFKDYKKLNKNYSKWIKDIADENNVAISKYVFNN
                     NEKLSIYTTDTSLNNNIKLKSGKFPNTNSNEFISNINTNNSNQVGKFSTMSKDISILI
                     RDFGKISEVGESGILYISTQNEDTINKILRELNVDRSIFVARLHDRYVNSNLYLNPSI
                     IRDLILIILCFLATIIHYSISVSREASILRLNGYSKLDIIARVIKNILRIMILSSVTA
                     LLIYIIYILKLNIGVRACLYFAMFSIICILINILISILIVGFNSRSSKYVLSLNGKKS
                     YGFVNIMHFTLKIVFVSFLILSINNCILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTG
                     EQSLGKEEVIKNAKIKSFYKSLVEEKDAFLIDTTNFEKLEDGSYMYEINSKGKNPEVS
                     QYGKNIKINKNYLKVNPIYSNGKEIFNLIDYNDKTINLLVPKKLQVHENDIKKEFREL
                     FYFEKIEVENMYNEKLNRGLNKTKESDLNVNIIYVDNNQSYFTYNSFVMEDNRNLIDD
                     PIVIIDIDNIDDSFYLSYISRCVYFNSKKLDALADISNIIESQGVEAYIQSLHAVYNE
                     YGLEINKLEKYLNSEIFTIIIIAISNLMITYNIVASYYERNKYKLYIKKIFGYSTTAR
                     SMLLIVSLILTNIIPISIISTRVDLSNNIILFGFLILVVEVIISIILDKIISNSSFNK
                     IIKGEH"
     gene            2072..2689
                     /locus_tag="KRH39_003742"
     CDS             2072..2689
                     /locus_tag="KRH39_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513608.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVSLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQEDRVIKL"
     gene            2878..3222
                     /locus_tag="KRH39_003743"
     CDS             2878..3222
                     /locus_tag="KRH39_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBG8513609.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDANGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
BASE COUNT         1393 a          294 c          475 g         1078 t
ORIGIN      
        1 ttatataacg atacaacaag tatatctgta atgtttaaag attataaaaa gttaaataaa
       61 aattatagta agtggattaa agatattgca gatgaaaata atgtggctat atcaaagtat
      121 gtttttaata ataatgaaaa attatctata tatacgactg atacaagttt gaataacaat
      181 ataaagttaa aatcaggaaa atttcctaat actaattcaa atgaatttat tagtaatata
      241 aacactaata atagtaatca agtaggtaaa ttctcaacta tgagtaaaga catatcaatt
      301 ttaattaggg attttggtaa aataagtgaa gttggagaat caggaatctt atatatttca
      361 actcaaaatg aagatacaat aaataaaata ttaagagaac taaatgtaga cagaagtatt
      421 tttgtagcaa gattacatga tagatatgtt aactcaaatt tatacttaaa tccttcaata
      481 attagagatt taatactaat tatcctttgt ttcttggcta caataataca ttattcaatt
      541 agtgtgagta gagaggcttc tattttaaga ttaaatggat actctaagct agatataatt
      601 gctagagtta taaaaaatat acttagaatt atgattttat catctgtcac agcattatta
      661 atatatataa tctatatttt aaaattaaat ataggtgtta gagcatgctt atactttgct
      721 atgttttcga ttatatgtat tttgataaat attttgatat ctatattaat agttggtttt
      781 aatagtagaa gttctaaata tgtgctatct ctaaatggaa aaaaatctta tggttttgta
      841 aatataatgc atttcacatt gaaaattgta tttgtatcat ttcttatatt atccataaat
      901 aactgtattt taaattataa tatgctacaa actcagttag ggaatctatc tgagtgggat
      961 aaagcaaaaa atgtttataa tcttactctt aaagatacag gcgaacaatc tttaggaaag
     1021 gaagaagtaa ttaaaaatgc taagattaag agtttctata aaagtttagt agaagaaaaa
     1081 gatgctttct taattgatac aactaatttt gaaaaacttg aagatggaag ttacatgtat
     1141 gaaataaatt caaagggtaa aaacccagaa gtaagtcaat atggtaaaaa tataaaaata
     1201 aataaaaact atcttaaagt taaccctatt tactcaaatg gcaaagaaat atttaatcta
     1261 atcgattata acgataagac tataaattta ttagttccta aaaaattgca agtacatgaa
     1321 aatgatatta aaaaagaatt tagagaatta ttttactttg aaaaaataga agtagaaaat
     1381 atgtataatg agaagctaaa tagaggttta aataaaacaa aggaatcaga tttaaatgtt
     1441 aacataatat atgtagataa taatcaaagt tattttactt ataactcatt tgttatggag
     1501 gataatagaa atttaattga cgacccaata gtaataattg acatagataa tattgatgat
     1561 tctttttatc tttcttatat atctagatgt gtatatttta attctaagaa attagatgcc
     1621 cttgcagata taagtaacat tattgaatct caaggtgtag aagcgtatat ccaaagttta
     1681 catgcggttt ataatgagta tggtttagaa ataaataaac tagaaaaata tttgaatagt
     1741 gagattttta ctataataat aatagcaata tcaaacttaa tgattactta caatatagta
     1801 gctagctact atgaaagaaa taaatataag ttatatatta aaaaaatatt tggttattca
     1861 actacagcta gaagtatgtt gttaatagtc tcattaatat taacaaatat tattccaata
     1921 agcattatat caacaagagt agatttaagt aacaatatta tcttatttgg atttctgata
     1981 ttagttgtag aagtaattat atctattata ttagacaaga ttatctcaaa tagttcattt
     2041 aataaaatta taaaaggaga acattaaaaa gatgatttca ttaataaata tatcgaaaaa
     2101 atttaaagat aaatctataa tagacaaatt taacctagaa atttctaagg gtgagtttgt
     2161 agcgataaca ggaaatagtg gttcaggtaa gacaacttta cttaatataa tgggattatt
     2221 agaaaaacct aattctggtg atattataat aaataatata aaaaatccaa atagtaaaca
     2281 gataagaatt ttacaaaggg atttttatgg ttatctattt caaaattatg ctttaattga
     2341 gaatgaaaat gttgaaaata acttgaaaat tgctttaaag tatcaaaaaa atatcaacca
     2401 taatgaaaaa ataaattacg cacttgagtc tgtaagtctt agaaaataca ataaaaaaaa
     2461 gatatacgaa ttgagtggag gagaacaaca aagggtagca ttagcaagag taatattaaa
     2521 agaatgtgaa gtaatatttg cagatgaacc tacaggcaat ttagataaaa aaaatagaga
     2581 catagtattt gatatactta aaagtctgaa taagaatggg aaaactatcg tatttgtaac
     2641 tcatgattta gagttatcgg agcaggaaga tagagttata aaattgtaat aatatcaact
     2701 ataataaata taagaaagag attacagcta aatcaagttt aataatacta cgaatagaat
     2761 ttaaacatta agtaaattag tagtttgtaa atataagtat aatatgatat cataaaggct
     2821 caaaagaaat tttatataat aagaaatcta aaaataacaa atggaggaca tataaagatg
     2881 gaaaatttac caaattgccc gaaatgtaat tcagaatata cttatgaaga tggaactctt
     2941 ttagtttgtc cagaatgctc tcatgaatgg gcattaggat tgggagctga agaagattca
     3001 aatattatca aagatgcaaa tggaaatgta ttaaatgatg gagattctgt tacagtaatt
     3061 agagacctta aagtaaaagg aagttcatca tctataaaga ttggaacaaa agtgaaaaat
     3121 atccgtttaa tccatgattc atcagatgga catgatatag aatgtaagat agatggtttt
     3181 ggagctatga agctaaagtc ttctgttgtt aaaaaagcgt aatattataa ataagctgta
//
LOCUS       DAEORZ010000255         3222 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEORZ010000255 DAEORZ010000000
VERSION     DAEORZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.261"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(303..524)
                     /locus_tag="KRH39_003744"
     CDS             complement(303..524)
                     /locus_tag="KRH39_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBG8513610.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            1104..1361
                     /locus_tag="KRH39_003745"
     CDS             1104..1361
                     /locus_tag="KRH39_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG8513611.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            1830..3068
                     /locus_tag="KRH39_003746"
     CDS             1830..3068
                     /locus_tag="KRH39_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG8513612.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1327 a          292 c          488 g         1115 t
ORIGIN      
        1 agtgtttcaa ttgtcaatat tagtatttta attgttgact ctagttattg ctgatataga
       61 aaatatttaa tttatttcaa ttaattagat taaagtaggg tagcatctaa aatagctaac
      121 atgtttaagt tgaaatacgt ttaatatatt aatttttcaa agcgttaata tattaaagtt
      181 ataaccaaaa ttgaagttat aatctgccaa atctatagcg tattaagtgc gttatataac
      241 ttaatctttt atataaagag ttaatttttt taatttgtaa agcagagtac gtttaaaaga
      301 tactatttag ataatgatgt actatctaat tttaaaatat ctacaatagg gttctcttga
      361 agacttgcta ttgcttctgc aacagcataa acatcttcat ttaatgcctc tgcttttaca
      421 tttgcaaatg atttactttt aacaactgcc ttacccttat catcaaaacc agcatccaat
      481 tttagtttta tactagataa attttttgtt gaaacaactt ccataaaatt acctcctaaa
      541 aatttaattt caaagatttg tcgactttct ttgttagcta taatactatc atctaagata
      601 atatcggagt taaacagtaa tttctaaaga ttttttgtgt aaatttttat aggaaaaatt
      661 ttactttttt gctgatgaaa aagtttacaa gttatttgtg aagatatgtt cttagtgttt
      721 tgaagtagta tttgtatata ttcatgctta gttgagatag ttttgtatgg attttcatat
      781 gtgtagagtt aaatgttagg tactaaagtt tatgttaaat taaatgttaa agcttatgtt
      841 aaaaatttta ttaaatttaa tctgtaaata aaaaagttaa aagtccaata ttaacatttt
      901 acttttaaac cattaaaatc tttattaaag ctaacaaagt tataagttat gagaaatttt
      961 attaaagtta atacaaaaat taattttaaa gctaagaaat tttttattag attcaataaa
     1021 actaataaat atagtttagt caataatgta ttaaataaaa actttgttaa gtctataaaa
     1081 atatcttaaa ggaggtgtaa cttatgaaga taaaagtgga ggatatacca gttcaatttc
     1141 atgcaatggt agaaataatc ggaattgata agttcataga ggttgccaag ttgtatggag
     1201 gaacaaatac ttatatacct acgtataagg ggctttttag atatgctaga aatagggata
     1261 tagtaaaaca atttaatgga gtcaaccaca atgaattggc tattagatat aatatgtgtg
     1321 taagcaatat taagcgaata ctcaatgaaa atagtgttta aatataataa aataatgttt
     1381 aaacataatg aaaatagcct tgttaaatgt aagagtaaag acaaagaaga tgttttataa
     1441 agttttttta gacaaattaa ctaaatttac tacttattaa tttaaataca ttgtatattc
     1501 tataaaaaaa tttgttaaat actggagata tattgagaat acaaagaaaa tggtcgaaaa
     1561 tatttgttgg ctaaaaaata gataaaatga tacaattgct tgtataaagg ggaaaaataa
     1621 ataaataaac tatataaata aagaaatagc aattaatatg acataatata tttataaaat
     1681 taagaatata aatagatatt ttaagggatt agttgttaaa ttaggaaata ttaaaaaaaa
     1741 tatatattta tatttaaact attttgttat ttatataaaa atacttatct agttactagc
     1801 aattattagt agaaatgggg gaaatagaga tgaataaggg attttacaaa ttaaattcag
     1861 tggtattgaa ctttgatatt aagaatgatg ttaacaagat aagatttaat cattatataa
     1921 tgagaaaaga aaacatacaa agacttcaag gaactctgcc aaacggacaa ttttatatga
     1981 cagtgagaag agctgctaca gatttacaac tttctatttc tactataagt aggcttgtaa
     2041 aagagttttt ggatttaggg ataataagat tagtgtctaa gggtgtaaaa ggtaatcgtt
     2101 ctgtatatag ttacgtaagt atagagttta atggaagttt atttgatgat agtaaatata
     2161 tggaacttga ctgtgtatgt aatgatgaat ttattgataa tagtggcata gagtttgatt
     2221 gtgatagcaa agataattct tttaatggag atataaatgt agaaactgtg cattgcatta
     2281 atgagtattt gtctgatgat gatgtagagc ataattataa caatttccat aacaaagata
     2341 ctataaagga taaagccatt tctaatatta gcactatcaa aaaatatatt gatgaatctt
     2401 ttgatacaaa taaatcagaa aattctaact tacaacagga gtcaataaat ttcaacttta
     2461 atgatacatg caatgaaata gataaaagaa cgaataatag tatagaaaat aatcaaaata
     2521 aaaggcctat gagattgagt gacatcagtc ttttaagtga taaaggtatg acaaataaaa
     2581 agaattactt taatcataat catggtatac aaaaagggac taaatctgta acaaagaaaa
     2641 aagaatcctt aaataaattt aataaaaaaa atatacagca aagcattata aatatgctaa
     2701 ataaaaaatc aggtaagaat tttaaaatcg attctccaat aagcataaaa cttatcaatg
     2761 aaaggctaaa ggaaggctat gtcttagaag atttctacaa agttattgag gtaaaagtgg
     2821 ctaagtggaa aaataccata atggaaatgt atataagacc agaaactttg tttagctata
     2881 agtttgaatc ctatgtaaat gaaaacatat ctatgaaaag tcaaagtaat gttcacccat
     2941 gtaaagaagg caatagtaat gatagcagta attatataca ttctgattat acaacatatg
     3001 atggcagtgt gcgtgagaga aaaaaatatg agaatgtaag atactggaat cctatatgtg
     3061 agatttaatt agtttaagaa tgtatttatg ataaataaga tattaataga gaatagtgag
     3121 ctatagcaaa tgatatatca atagttggta gtaagttata ttaagcaaga tattactatc
     3181 aggtagtgaa ttatattgaa cgatatatat aattggcagt aa
//
LOCUS       DAEORZ010000256         3197 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEORZ010000256 DAEORZ010000000
VERSION     DAEORZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.262"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(174..851)
                     /locus_tag="KRH39_003747"
     CDS             complement(174..851)
                     /locus_tag="KRH39_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG8513613.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(1040..2404)
                     /locus_tag="KRH39_003748"
     CDS             complement(1040..2404)
                     /locus_tag="KRH39_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8513614.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTIFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            2532..3053
                     /locus_tag="KRH39_003749"
     CDS             2532..3053
                     /locus_tag="KRH39_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG8513615.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         1245 a          504 c          445 g         1003 t
ORIGIN      
        1 ctaaattatt aaaatataaa aatatacata aaaaataaat gtaaatatca aataagatac
       61 tatatcaaaa tagatataag tatctttttt attattgaaa ttatgaaaat atatttgatt
      121 ttacacaaaa aagagtgcct catgaactaa aaagttcatt ttgagacaca cttttacagt
      181 gtcaaagaac gaagtttatc cttatccaat acttttatac caccacgaga taaagacaca
      241 ataccttctc tagcaaaata tttcaacatt ctcgatacaa cttctctggc actacccata
      301 tacttagcaa tttgttcatg agtcatattt atagtgctac tgccattttt agctatctca
      361 tcaattaaaa aagatgccaa acgcttatca aaactcataa aaagaatttg ttgcatagcc
      421 cacataacat cagaaaaacg ttcggtagct aatttatatg aaaataactc tgcatgaata
      481 ttttctgtag ataattttgc aaaaacagag gaactaatct gtataatatc gcaatccgtt
      541 tcagcatcta catagacatc aaaagttatt gtttttagaa tacaggaagc tgataaaata
      601 catacatctc catcatcaag tcggaacagt gtaacctctc taccttcatc tgaaagtatg
      661 tatgtacgta ctgttccaga ctttataatc aaaataccaa tacagtcact atcaccacaa
      721 tgtactggat tccctttttt gtaatgcgag gtatttgctg aagaaattaa taattctttt
      781 tgtaagtcag taagtttatc ccaaaaaggc aagctatgtg taagtatatc aatttcattt
      841 tgtgaaatca tacaatatct cctttcttaa attgttataa tcccatgata caagtgaata
      901 gataaattga caagtagttt gttaagagga ttatttagtc agatagtaat agtatgattt
      961 atgtagaaaa aatatgataa tatatattct tgctattaat gagaaagtaa tatgttttga
     1021 tgatagtagg tttgtataac taatgcatac cgtttatttt taaaagatta agttccttat
     1081 gtaaccttat agacataaaa ccagcaatta tagcagccat caaatcagct attggttgtg
     1141 catataagat accatttaac ccatagaata caggaataat caaaattata gggataaaac
     1201 agacaccttg tctacacata cctaaaaatc caccttcctt tgcttttcct agtgcgagga
     1261 ataatgtaga atacactgta tagaatccaa atagcataaa tgaaaaccca tttgccctaa
     1321 gagctttact accattttct attaataaaa tgtcaccact tgtaaattgt gaaattatag
     1381 attttgggaa gagtatcaat ataagaccaa gcactacaca aaatattgta gtccataata
     1441 tggaagtttt tattgctata tgtaatctat cgtagttctt tgaaccaaaa ttataaccag
     1501 caattggttg gaaacctttt ataaaaccaa atacaacaag actacctaaa gacgttattc
     1561 gtgttacagc acccattcca gctatcacag aatcaccata ctttgtagcc tccatattag
     1621 ttaatgtaat ggcaagacta gtcaataatt gaaatattaa ggtaggtatg ccaattttta
     1681 ggatttctga aaaaatttct tttgaaaaac agcattcgtt aattctaaaa ctaaatatac
     1741 ttttcttgct taaaatatat cctacataaa caagtgttga agcaatctgt gagatagctg
     1801 ttgcaataga agcacccctt acccccagac caaaagtata aatgaatatc gggtctaaaa
     1861 tcatattaag caaagcacct aataacatag cacacatagt tgttttagca gctccttcac
     1921 ttgtgacaat attagtcata gtgacattga atatattaaa aatagatgac acgacataaa
     1981 tgctagcata agatattgca taaggcataa tactttttgt agctcctaac aattttaaaa
     2041 ttggattgag aaaaatcata atcacaataa taactaatgt acccactata atactactgt
     2101 aaatagatgt acttaccact tttcctgctt tttcattatc accatgccct aaaagtctgg
     2161 caatatatga agcagctcca tttccaaaca ataatcctaa tcccacaaca agctgaccta
     2221 atggaaatgc aacggatata gctcccattt gacttgttcc aagtccacca acaaagtatg
     2281 catcaaccaa attgtataat gcagttatca tcatacctat catggtaggt aatcccatag
     2341 ataacaatgc ttttgagatt ggggcagacc ctaataattc aattcttttg ttactttctt
     2401 tcatacttaa cactcctctt ctattgacat taagtctaaa ttataatact ataaattata
     2461 gtaagtatgg aaaatagtac tataatttat agtatttgtc aatagtaaga aaactaaagg
     2521 agatgatttt tatggctaca atagatttaa ttgtattggg aatagtaaag caacaaccca
     2581 agagtgctta tgatattcaa aaggcagtgg aatacaggaa tatatcacgt tgggttaaaa
     2641 taagtacacc atcaatttat aaaaaagtaa ttcaattaga acaaaaggga tatattgaaa
     2701 gtaaaactgt gaagaatgga aaaatgccag aaaaagcagt ttattcatta actgaattag
     2761 gtgagacaca ttttttagat ttaatgtttg atacatctag caaaccaata aatattcttt
     2821 tagattttaa tgctgttata gtaaatttaa atagccttac accagaaaaa cagaaattat
     2881 gtctcaataa tattgaagag aatgttcaag agttaaagaa aactattgag gaaaatataa
     2941 gtataaagac agaagtacca gagacagcaa aagcagtgtt ggagcaacag ttggtgctag
     3001 cacaagcatt ggaaacctgg ataaattctt taaaagataa tatggggaat tagaattaga
     3061 gaagctaact taataatcat taaacaaaaa ccagcatatt taataatgag atattagaaa
     3121 aaataaaaca aatgatataa actgaaaagt atatttaaaa taaagctcac ttaattgagg
     3181 gagaatgtta aactttt
//
LOCUS       DAEORZ010000257         3165 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEORZ010000257 DAEORZ010000000
VERSION     DAEORZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.263"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            65..1000
                     /locus_tag="KRH39_003750"
     CDS             65..1000
                     /locus_tag="KRH39_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513616.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSIIDKNDSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            1014..2111
                     /locus_tag="KRH39_003751"
     CDS             1014..2111
                     /locus_tag="KRH39_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513617.1"
                     /translation="MKLYMSVKTMLKGLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            2114..>3165
                     /locus_tag="KRH39_003752"
     CDS             2114..>3165
                     /locus_tag="KRH39_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513618.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRD"
BASE COUNT         1212 a          371 c          422 g         1160 t
ORIGIN      
        1 cacaattctt tattataata ttatagcttt ttaacttata caaaattatt ttggagggat
       61 tactatggat atagtcagag taaataatat aacaaaaaga tttaatgata aattagtact
      121 tgataacatc agcttttctg ttaaaaaagg tgagatattt ggacttattg gcccaaatgg
      181 tgcaggtaaa tctacactta taaatataat tacaaatctt atgttaccaa atagtggtag
      241 tattcaaatc aatggtttag atttatcaaa agattatata aaagcaaagt ctataatagg
      301 gttagttcca caagaattag caattataga aactcttact ccttttgata acttagagta
      361 ctttggagct ttctacggtt taaagggaaa actacttaaa gaaagaatca ttgaagcatt
      421 agaagttaca ggtcttactg aagtcaaaaa gaaaaaggtc aaaaaattat ctggtggtat
      481 gcaaaggaga ttaaatattg gaatagcact cttaaatcat cctaaaatat taatattgga
      541 tgaaccaact gtaggtgtag acccccaatc aagaaatcat attttcaatt ttataaaaga
      601 tatatctaaa aaacatgaaa ctactgtaat atatacttcc cattatatgg aagaagttga
      661 gcacctttgt tctaaaattt ttattatgga tgaaggtaaa gaaattgctt ttggagataa
      721 tgattattta aaatcactcg tatctacaaa tacaaaatta attatggaaa taaaaaatat
      781 aaatgcccaa cttatctttg atttaaaaaa tactaaaggt gttatatctg ttttagaaaa
      841 taattcatta ttagaattgg atatagataa aaaattacaa cttacagata tactctctat
      901 aattgacaaa aacgattcaa aaataatgaa aatttcttat gaagaaccta gtctagaaga
      961 tgtattttta aatctaacag gcaaaaattt aagggactaa tggaggtgat tttatgaaat
     1021 tatacatgag tgtaaaaact atgctaaagg gtttaaaaag ctcctttata cttaatttaa
     1081 tatatttttt agctttacct ctgattttat cttggtttct tggtatggtt acagaaagca
     1141 tgtttcaaaa ccctataaaa acagaatcta cccccattgt tatatatgac aaagataaca
     1201 caagattatc aaatgatttg acaaagtatt tgaaaaatga tttatcttat atattaacag
     1261 ttaaaaaaga tgattctaag gcagaattaa agcttactat acctaaaggc tatgagagta
     1321 gtttattaaa tgaaaaatca aatactctaa acatagaaaa acttggaact cgtgatgata
     1381 ttgcaatatt gcttcaagat atattagata cttaccatga aaaattttat ttaaataatt
     1441 ctcagaaaat aagttcagaa gatttttcta aattatttaa taaaaattct attgatactt
     1501 ctataataaa taacaatgtt aaacagagct cttatgaata ctttgcttta gtttcactag
     1561 gatttcttgt aataattttt ataatgaaca atattttatc aaattatata agtgaatcaa
     1621 agggactttc aaaaagactt tattctatgc ctataactag agtacagttc ttaatttatg
     1681 actttgtagg tttatggatt tattcattta tattcttatt attatatgtt ttattcttta
     1741 gaatacttgg aataacattt aaaggtaatt ttgccatatt gctcctactt tgtgctttat
     1801 catcttattt tatgacttca atatctacct ttgtaaccag cttttttagc aaaaaatacg
     1861 gtacagcaat agtttatgca ttactctttt tacagacaat ttttggaggt atcttcagta
     1921 tgataagtga tacatttact aagcttacaa gtttatctcc tacctattta ataggagaat
     1981 tgtttagtaa ttatgaaaca tttaaaacta tagattcaat aggagattta attttcacat
     2041 gtttaattat ctctacaata ttaattgcac ttgctattgt aaaggaaaaa tataaatgga
     2101 gggaggttta ataatgatta gattaactat tgcaaatata aaaagatatc taaaaaatca
     2161 tactctttta attaatatgg ttatgcttcc tattatacta attttttcac ttaatttttt
     2221 tatcaacaac tcaggaaatc aatcaatgta cttttctcca gtagcaattg tatctgactc
     2281 ttcaggaaag tatgaacata agttaataaa tagttctaaa ttaaaagaaa attcatttag
     2341 attaaatgaa caagataagg ctatgaatct attaaaaaat aataaggttt ctgctgtatt
     2401 cgttttagat aaaaattttt caaatagtat agataaatta caacgacctg tagttaaatg
     2461 ctttaaaatt gaaaatggtg gtggtagtct ttgggcagaa tcacaaatag aatcttttat
     2521 tactaaatcg ttaaaattga aagtggataa aaatatagat gataaattga caaagacaaa
     2581 tataattgat aacaaaccag ataagaataa aggttcattt ttagtagtct tcttgatatg
     2641 ctattttatg tatattaatg cggcacatct tgcttctgat ttatttacat taaaaaaatc
     2701 aaatgtgcta aaaagactta tctctacaga taataaagat ataaaaataa ttttcagtat
     2761 atttttagga ctatttttta ttcaatcaat tgtatacact tttgctctat tatgtttttc
     2821 aattatagaa gactttaatt tgagtctgaa tgtattgatg attgtactag caaatagttt
     2881 tgtttctact ggatttgtat tttgggtagc tagagtattt aaaaatgaaa gttctataag
     2941 tcttatatct acattttatt ctttaatagg tctagctata tctatctcta gtctaatacc
     3001 atcaatggat aaattatcct ttatgactaa tctttcaaag ttcacaccat tttattggac
     3061 tatagatgct attaaaaaca atggtagtat tttaataaat ataatagctc taatattaat
     3121 tggaattata tttgtaactg caggaagtct taaactaaga gattt
//
LOCUS       DAEORZ010000258         3093 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEORZ010000258 DAEORZ010000000
VERSION     DAEORZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.264"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            103..993
                     /locus_tag="KRH39_003753"
     CDS             103..993
                     /locus_tag="KRH39_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8513619.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLSPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            1211..2965
                     /locus_tag="KRH39_003754"
     CDS             1211..2965
                     /locus_tag="KRH39_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBG8513620.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVNIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         1296 a          274 c          534 g          989 t
ORIGIN      
        1 ttaataataa aagaaataaa aatatattat aaaaacaatt tgattggaat tttcagaaaa
       61 ttaatgctat aattaaaagt aaatttaaag ggaggttata aaatggctaa attaactcta
      121 gaacatataa aaggaaatac atattatatt ccactcccta caatagtagg tgtatacgta
      181 gatggtaaag atgcaatttt aattgacagt ggaaataata aggatacagc tagacaagtt
      241 ttaagattat tagaagaaca taatttaagt cctaaattaa taataaacac tcattcaaat
      301 gcagaccata taggaggaaa tgcatatttg aagaatcaaa caaaatgcaa gattgctacg
      361 actaaaattg aaggatattt tacagaaaat ccaatattag aatcagcatt tttatatggt
      421 ggctatccaa gtaaagcact gaagaataaa tttttattag caaaggaaag tgaagttgat
      481 tatattatac cttcaaatgg caaaattgta gatacagaac ttgaagcgat ttctcttcca
      541 ggtcattatt ttgaaatgat tggtgttaga actccagata atgttttgtt tgttgcagat
      601 agtcttacac cagagaatat aattactaaa tatcattttt tcttcctact ggatatagat
      661 tcacagttta agacactaga aaaattgaga atgttagagg ctgatttctt tgttccaagt
      721 cactctgtca aaactacaga tataaaaagt ttgattaata ttaataagaa gaagatggaa
      781 gaaataatag ataatattaa aaaggtttgt tatgaaccag ttatgattga tgaagttatt
      841 gaaaaaatgt gtgatttgta caatgtcaag cttgatgcta atcaatatgt gttggttgga
      901 agtacaatac gttcttatat aacttattta tatgagaata atatggttga atatatattt
      961 gatggtggta agatgatgat aaaagctgtg taaatagtaa taattttcaa attatatgat
     1021 gaaattgttt tagtatgact gttatttgaa aagtaatata aaaaaattta tataaataag
     1081 ctaagatgat tgatataaag ttactgataa ttttaaggta atatatacgt tactataata
     1141 ttatgttatt atttgtaaat aaggggataa tttataagca aattaaagac cttaatttac
     1201 aggaggaata gtgtatatta tgaataaaaa attcaaaaaa ttcagtaaaa agctaagtat
     1261 attaatttta gttttgacta tgtttctttt taataattta actgcaaatg cacagattaa
     1321 agaagattca ataataggaa ttgatagata tgaaacagca ggacttatag cggatagaca
     1381 aacttatgat actgtaatat tagttaatgg agataaaaat ttatctgatg ggctttcatc
     1441 aagtggatta gctggagcta ttaacgcacc aatacttttg actaaaaaaa gtgaaatacc
     1501 aaaagctact ttaaatagac tagataataa gagtttaaat gttgtaaaaa aagtaaatat
     1561 aattggtggt tataatacta ttgaaaattc agtagaaaaa gatataaaaa gaaaaggtat
     1621 tcaagtggaa agaataaatg gtaacaatag aattgaaact agttataatg tagctaaaaa
     1681 aataaatgaa gtaagtagtg taaaagaggt aatgcttaca aatggatttg taggagaggc
     1741 tgatgctatg agtatagcac ctgtagttgc aaaaaataaa ggagctataa tacttacaga
     1801 tgggaaatca gttccattta agactgaagg tttaaatgtt tacgctatag gtggcaaatc
     1861 tgcgataaat gaggatttag taaatgaaac taaagcaact agaatcggag gaaatgatag
     1921 atttgagacc aataaaaagg taatggaaaa gttttataat ggagcgacag atttttatat
     1981 aacaaatgga tatcaattag tagatgcact aacattatct cctttagcca aagaaaaacc
     2041 aatcgtttta gttgctgatg gaagtaataa aggtatttta aaaggagcta aaagtataac
     2101 aaaagttggg ggtattgatg ccaatatata taaacagtgt ttagatatga ttaaatataa
     2161 taatatggat atgactccaa atattttaaa acacttaact tcagttgaag gaaatgaagt
     2221 ttctgaaata cttatagaat cagataattt aggtgtggtt gttgaaaaga cgaataccaa
     2281 taaatttgag tttgattacg taagtgtaac taatgaaaaa aattgtactt tttctgtata
     2341 taaagaaaat tcttctaata atataaaaaa tggaaagcta gttgtaagtg ctaagaaaaa
     2401 aatagataag aaagatagtg atttgcaaga tatgaatggc gacaatatga taaatgcaaa
     2461 taaagatagg atggtaaatg tgataaaaat tggagttccc gataaaatgt attctaattt
     2521 taaagtagat gttgaaagtg gttcagtaga actttataat attaaaggag gagctactgt
     2581 tgatgtcaat gacggaattg ctaacattgt tgataacaat gtaacaaatc ctttcaatat
     2641 aaatactaat agtgggattt caggtgtaac agctgaaact atatcatctg aaataaaatt
     2701 tagagcaaac aatggaattg taaatgtaac agctacgaat ataaatggca atataagttt
     2761 atttggaagc gataataaag gtacctttga tggaatattt aaattaaatc tgaaaaaaga
     2821 accatcaaat ttacacttga agttaattgg aaatgggata aataaattgc cagaaggttg
     2881 gtctaaagat tatattttag gaaatggaca accaattatt gaagttaaaa ataatggcat
     2941 aaataatata tcaattggag aataaaaaca tatgaaaata aagtttatat agttttaaaa
     3001 attatagaat tttaaaatat ttatatatat tctttaaata aaaaattatt ttttaagtga
     3061 taattaagta tttatcgata aggctatggt tgt
//
LOCUS       DAEORZ010000259         3043 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEORZ010000259 DAEORZ010000000
VERSION     DAEORZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.265"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            25..819
                     /locus_tag="KRH39_003755"
     CDS             25..819
                     /locus_tag="KRH39_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513621.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            820..2880
                     /locus_tag="KRH39_003756"
     CDS             820..2880
                     /locus_tag="KRH39_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG8513622.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSISTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         1159 a          314 c          501 g         1069 t
ORIGIN      
        1 aacgagaaag gagtggtttg ataaatgatt gaggtagata aattgtgtaa gtcttttaca
       61 agggttgtaa aagatgataa gaataaaagt tcaataagaa agctaaagaa agtaaaaact
      121 aaaaaagaag aattttttgc tgtaaataat gtttcattta aagttatgga aggtgaaatt
      181 gttggaatat taggtccaaa tggtgcagga aagactacat tacttagaat gttaggtggc
      241 atattaacac caacatcagg aagtataaat atttctggat atgattattc aatagataga
      301 aattcagcaa aaaaagaaat aggttatctc tctggaaata caaaactata tggaaggctt
      361 tctccgagag aacttctaac aacttttgca agtctatatg agatgtcaaa agaagatata
      421 gaagaatcaa ttgaaaatat tgtaaaaata atggatatga gtgaatttat agataataga
      481 attgaaaatc tatcaactgg tcagactcaa agaacatcta tagctcgttg tttaatacat
      541 tctccaaaag tatatatttt tgatgagcca acactaggtc tggatgtttt aagtagtgca
      601 tctattatag attttatgaa gaatgaaaaa ctaaggggaa aaaccgttct ttactcaact
      661 cattatatgg aagaagcaga aactttatgt gacaagattt ttatgattca caatgggaaa
      721 ataattgcat caggaacacc acagagttta aaagaggaat ctggagtaaa taatttgaga
      781 gatgtattca taaaacttgc cagaaaggag gacatttaaa tgagaagtaa gattgtaaaa
      841 tacatattta aaaaagaaat gttagacata ttgagagata aaaagacttt atttatgatg
      901 attgttgtgc cacttctttt atatccttta cttatgttat taatgattca agtaatgaat
      961 atgtctgcaa gtagtatgaa tagtaaggac attaatatag cttttaataa taaacctaat
     1021 aaggtgttag tatccatgat tgaagatgat aatttagaaa aagatactaa aaatacttca
     1081 aatgataaag gcaaaataag agtgttagat gtagataact ataaaaagtc attagaagaa
     1141 aataaaattg atgcttatat aaagatggaa gaaaaaaaat catctataaa ttatcaaata
     1201 tatataaatt cttctcaaga aaattcttct aatgcactaa aaagaattga aactgttttg
     1261 gataaatata aaagatttac catagaaaaa actttatctg aaaaaggatt agatgtacaa
     1321 gctactttag aaccaataac atatagcact gtagatgttg caaagacgga agaagtggca
     1381 ggatactttt taggtcagat acttccattt atattaataa taggagtttt actaggttca
     1441 atatatccag caatagatgt tatggctgga gaaaaggaaa gaggtacatt agaaacatta
     1501 tttacactcc caatatcaaa cttagaactg gttatgggaa aatatatggc agtttctctt
     1561 agtgcaatag taacagcaat tttaaacatt ttatctatgg gactgactat ggcatttatt
     1621 ttagttagtg gaggtattag tagccaattt ggctttggaa actttaatta tggagactta
     1681 gtcttaccaa ttataactac tttaatttgc atatgtcttt tttcaatggt agtttcagca
     1741 ataagtatgt gtgtatgttc attagcaaaa agttttaaag atgctcaaaa ctacataaca
     1801 cctcttatgt taattattat gataccatca tatgtttcta tgataccaaa tatagtatta
     1861 gatagagtaa cagctgtaat acctgtagta aatatatctt tacttataaa aagtgtatta
     1921 tcttttaaaa gcgatttaaa tttaatagcc ttagttttag catcaaatgt agcctttgtt
     1981 atattggcaa tcatactact gtctaagatg ttcaattctg aggaaatact ttttggtaat
     2041 agtaagagtt tctctttctt agagaaaaga agtaatataa aaaaagggtc tatatcaact
     2101 gtaagtgatg gtgttatttt atatgcagta ggattagttc tattgattta tgttggttct
     2161 ttagttcaaa ttaaatttgg tatggcaggt attataatga cacaattcat gataatttct
     2221 ttaccgttat tatttgcata ctatattaaa gctgatttta aagaggtatt tagcttgaaa
     2281 gttcccaaat taaagcatgt gtttgggagc attgttcttt ggattggtgg atacatactt
     2341 attatgatta taacacagct aattttattc ttatttcctc aaaatatgga agttgctgaa
     2401 agtttaaata aggctctttt tatcaaagat agtgttttac tgaatttact agcaatagct
     2461 ttattaccag ctatatgtga agaaatgttt tttaggggat ttatattttc ttcatttagt
     2521 aaatcaaaag ataaaaataa gtctatcaag ctagctataa tatgcagtgg ggtactattt
     2581 ggtattatgc atatggattt tattagaata attccaacat caatattagg tattatattt
     2641 gcatatagtg tgtataaatc tggttcaata tttgtatcaa tgttattgca ttttttaaat
     2701 aatagtgtag cagttgtgtt aaatcattat acaactggaa atataaccaa cttatataaa
     2761 tttgttgaag ttgatttttc gaacttaaat gtacttaaat ttgccttaat tcttctaata
     2821 agtcttatat taattatgtt aggattaaga gttttagata gaaagtcttt aagaaattaa
     2881 gtataaagtt attatataga ttaaaatatt aataagcact aaagtatatg gatatatctt
     2941 gtatataaaa atactttagt gctattttat atattttaat tatatgaatt atttatcgtt
     3001 tcatttatgt aatgaaaatg ttatataaat gaaacaataa ata
//
LOCUS       DAEORZ010000260         2973 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEORZ010000260 DAEORZ010000000
VERSION     DAEORZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.266"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            69..1256
                     /locus_tag="KRH39_003757"
     CDS             69..1256
                     /locus_tag="KRH39_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8513623.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGLASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            1282..1974
                     /locus_tag="KRH39_003758"
     CDS             1282..1974
                     /locus_tag="KRH39_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG8513624.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(2191..2493)
                     /locus_tag="KRH39_003759"
     CDS             complement(2191..2493)
                     /locus_tag="KRH39_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBG8513625.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(2515..2925)
                     /locus_tag="KRH39_003760"
     CDS             complement(2515..2925)
                     /locus_tag="KRH39_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBG8513626.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
BASE COUNT         1025 a          466 c          350 g         1132 t
ORIGIN      
        1 aaagtacttg attaaaaaat ctaaatggta tattatttgc attataaaaa taccatacgg
       61 aggataatat gaaaaaaata tctaatcaaa aattactttt actttctatt gttacatttt
      121 tctggttttc acaatatgta tatatacctt tccaaaatcc atatttaagt gcatgtaatg
      181 tatctacaaa tatgattggt ataataatag gtgcatatgg aatctcacaa tgtttattaa
      241 ggcttcctgt tggagtattt gctgattcca taaataaaca taaaatattt atactgtctg
      301 gaattttctt tgctggttta gcatctctta taagagtctt ttctcctgga ggcaatggtt
      361 ttcttatagc taacatttta tctggaatat catcatcaac ttggatttct tacatggttc
      421 actataccaa ctttttttct acagaacatc aacaaaaagc tacaagtacg ataatattgg
      481 taaacaatct aggaatgttg attggatttg ttatgagtac tttattttat gaaaaattag
      541 gaatgacagc cttatgtatc atgagtgtat tgtcttcagt tattggaacg attctagcct
      601 taaatataaa aaatcataat tcaaaaaaaa tacctgaaaa aatatcaatt aaaaaatatt
      661 tatctatctg caaggataaa agacttgtat ttttttcctt tatggcatta attcaacagg
      721 gtattcaaat gtctacaaca atgtcattta ctactcaaat tcttaaaaac ttaggttcta
      781 gttctaccct cataggtcta tcttctatat tttacatggt gtgtgctgtt gcttcttctg
      841 gccttgcttc tactaaattt tgtactaagt atggaccaaa attttggatt cctttagttt
      901 ttattttagt ttcagcctat tgctttttag ttccaacaat aaaaaatatt aatataatat
      961 ttgtactaca agtactacca ggtatatcta caggtatact cctttcttac cttactagcg
     1021 agtctatgaa aaatgtacca aaacatcaga aatctactgc aatgggtttt tttcaagcaa
     1081 tttatgctat aggaatgtct gtatttccaa tgatagttgg taaaatatcg gaatatactt
     1141 caatacaata tggatacttt atcttggcta ttatagctat tttggcctct atcctgtctt
     1201 atacatatta taaaaaagta gatttaactc ttaactatga tataatacaa tcataaaaaa
     1261 attatatgtt taggagtttt tatgaaaagc ttaaatgcca ctgaaaaaat tattgattat
     1321 attaaattaa acattctaaa tggaactttt aaaattaata gtaaattacc aagtgagaga
     1381 aagattgctg acctatttaa cataagtaga atacctgtaa gaaatgccat aaatatactc
     1441 tgtaaggaag gaatacttag agctgtgcct tattcaagtc ctatagttga aggatttaaa
     1501 aaagttgacc tatttgatga tggtgaaatc tataaaaatc ataacataca agaattttat
     1561 gtagaatctt taagagcaag acaacttatc gaaagtgaag ctactaaact agcaatttta
     1621 aatgctacat ctaaagaact tcaaaaaata aaatatactt acctaaagtc aatagaggaa
     1681 ttggataagg tttctcatgg tttgatagaa gaatgttatg atgctgactt gcaatttcac
     1741 aaagagataa ttttagcttc tcataatcca atttttataa aatattatga gttaatacct
     1801 aaaacagttt cttctaacca atacttcggt tttaaatata gaaattctct caaggatatg
     1861 attagccacc ataacaagat aatgattgca tttgatagta aagactctta cttaggttat
     1921 acatcaatgt acaatcattt agaagatgta attcagttat ttcaacatga ttaaacttat
     1981 attttacctt aaatgcaact aacatataaa aatggagtgt ctcaaagtga actttttagt
     2041 tatgaggtac tcttttttgt atgaaatcaa atatattttc atcatttcaa caatgaaaaa
     2101 gagtcttatc tatattttga tacaacctct ttatttcttg tgtaaaatat ataaaaaggc
     2161 gtcgctctat tcttagtgcg acacccacga ttaatttaag tctattaaaa tttcacttac
     2221 tatagatttt gcatcctcta ttatatactt tggatcaact tcataaaatt ctctatctat
     2281 aaatctatca atcttctcta tgctttctat agaatcactt gtaacaaata acactatgga
     2341 tgcatcttct atatcataaa tagaaagttc attttttata ttatcatttt cttgaatttc
     2401 ataaactaaa tcaaatccta aatcatttgc agctgtctta agagaattta aacttctttc
     2461 actttcacaa actgctacta tctttttatt catatttcaa atacctttct aattctactc
     2521 cggtacaatc atcactatac attttgcttg tttaactaca cttgctgtta ctgaacctat
     2581 cattcttgta agaccttttt tagtagattt tgtcataact ataaaatcta cattatcttc
     2641 ttttgctttt ctaattatct catctcctgg atatccaaaa gtaaaatgta ctttaacatc
     2701 atactctccc attatctctg ctgctttatc caagattctt cttcctaaat tttcagaatt
     2761 ttttatctct tcagccaggg atatgccatc aataaatact agttctttta cattcattat
     2821 ttctacttca acttcatctt ttttaaatat tccttttata aattccaaag aatgcatact
     2881 tctttctgtc ccatcaattg gcactaatat tttcttttta ttcatgtcac atcttccttt
     2941 ctcactttag gataattaaa taaaatatat aat
//
LOCUS       DAEORZ010000261         2950 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEORZ010000261 DAEORZ010000000
VERSION     DAEORZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.267"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            185..1294
                     /locus_tag="KRH39_003761"
     CDS             185..1294
                     /locus_tag="KRH39_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG8513627.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1671..2855)
                     /locus_tag="KRH39_003762"
     CDS             complement(1671..2855)
                     /locus_tag="KRH39_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513628.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1140 a          442 c          406 g          962 t
ORIGIN      
        1 tttaaaaata aacctataaa attcctacta aaatttaatt taagttacat tccattttaa
       61 agtatcttaa tatattttta agctatataa ataataatta aaaaatttac taaaaatact
      121 tataaatatg atattattaa ttggttaata aaaaagaaaa tttttcatga aaggtaagat
      181 aacaatggat ttcaaaagct tagggattag tgaaaataca ataaatattc taaaaaaatc
      241 aggaataaca acacctacac ctatacaaaa agaaagtatt aaactaataa aagaaggaaa
      301 agatgtaata gcagaagctc aaactggaac aggaaaaaca ctagcttttt tacttccgat
      361 atttgaaaat atatcattag atataaatga tatacaagta cttatacttt ctcctacaag
      421 agaactagct attcaaataa cagaagaagc tatgaaactt aaagaatcta aagatgtaag
      481 tattcttgca gcatatggag gaaaagatat tggttctcaa ataaagaaac taaaaggaaa
      541 tattcatatg attatagcta caccaggtag gcttttagac catctaaata gaaaaacaat
      601 agaccttagt aagttgaaga cttttgtttt agatgaagca gaccaaatgt tgcttatggg
      661 atttaaaaat gaagtggaag ctattttaaa agagacttca aacaaaaagc aaacactatg
      721 cttttctgca actataaatt ctcaagtaaa aaaattagct tatagataca caaaaaatcc
      781 tgttgtagtt tctattcaaa aagaagaaat cactcttaat aatataaaac aagaagtagt
      841 agaaactaca gatagaaaaa aattagatgc cttatgtaag gtactagatg aagataatcc
      901 ttttatggca ataatatttt gtagaactaa gagaagggtt gacaatcttg aagaagctct
      961 tgctataaga ggatataatt gtcaaaaact tcatagtgat attgctcaat caaaacgaga
     1021 aagaataatg aaatcattta gaaatttaga tatacaatac ttaatagcta cagatgtagc
     1081 ttctcgtggg cttgatatat ctggagtaag tcatatatat aattacgatt taccagaaac
     1141 accagaagac tacatccacc gtattggtag aactggtaga gctggtgaag aaggctatac
     1201 atgtgctttt attgacccta aaaatgaacg aatgttaagt gaaatagaga cggctataga
     1261 atctaaaatt agtagaagaa ttatagaatt ataatttata gattgcttaa aataccaatg
     1321 ccacacaaac actgtaatat atagaaagct acagaaaaca tgtatcaatc tatactttgc
     1381 tgttatacta ttttatatta aagaaaaaag aggctatcca aagtaggatt agtctctttt
     1441 attgtcaaaa taataaaaat atatttgact tcatataaaa aagagtgcct tataaactaa
     1501 aagtccattt tgagacaccc tctttttatc ttttattctt agttttatat tgcttgttta
     1561 tagttgttta atattagatt ttgcaaataa tcttaataaa catgcagata tattatctat
     1621 agtattctta tctatagtat tctctttagc aatatttttt gctactacat ctaatttaca
     1681 ttttcattta attccgctgg ccatttagct tctggatttt cctttgcaaa cttcttattc
     1741 ttataatgtc ctagtgtaag gaatggaatt actatagtga atagaccaag ataaccacaa
     1801 tatccatatc catatttaac aatattactt aatcctgtaa gtgatataag cattgacact
     1861 accattattg cacaagctgt aaataccctt cttttcattc ttgaagaaag gaaagaaagt
     1921 gctttatggt cttcaaatct atttacaaga ccaaatatag tagttactcc tgtagacatt
     1981 aaacaaagga ataatgcaac attataaatt atgtatatag tatgatttcc agattgacct
     2041 gcaacataaa gagtagggag tgttgtttgc ccagcagcaa taatttctct ataccatccc
     2101 attagcatag ttacagcaag tcctaatgca attgcattca taacaaatga aattatcatt
     2161 gccttagtag cttcttttga gttcttaaga agtggtcttg aacatcctgc aagtgtaggt
     2221 ataacaaccg attgaaaacc tgcataaata aaagtattaa gtattggctt gatatagcca
     2281 ggtgtgaaac tcgttctatt tgataatatt tcagttattt ctggtacttt agcttttata
     2341 ccaacaataa aaatagtaag agtacatact agaatagcta ttgtcattgc tgtagctgct
     2401 ttagatacaa gcatagcacc aaatatagta aagacaagaa gtacaactcc aataataaat
     2461 acagctacaa aataatttac tcctatactt tgaaatactg ctgctgcacc tgctataaca
     2521 gcacttacag ctattaaaac cattagatag taatatatct cccatattat ttcaagtttt
     2581 ggatatggtc tccaaagctc acaaaataaa tccttataat tcctacaatt gttattatta
     2641 tacattatga taacttctct catggtagca gtaagtataa tcattgctaa aattgccata
     2701 agtggtgctg tccatccata ctgcacatag tactgagttt cttgattccc tgtagcaaat
     2761 cctcctcctg catgtgaact aaaaagaacg cttgcaacac caaaaatggc taacatagag
     2821 cccgttactt ttgctttctt tacacttttt tgcatgaaat catcctccct aaaatgatat
     2881 taaaagtttt tattttaaca ctttattgat aaaacaataa catttttgct cattaaattc
     2941 aactatattt
//
LOCUS       DAEORZ010000262         2942 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEORZ010000262 DAEORZ010000000
VERSION     DAEORZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.268"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            137..331
                     /locus_tag="KRH39_003763"
     CDS             137..331
                     /locus_tag="KRH39_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBG8513629.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(433..942)
                     /gene="vanZ1"
                     /locus_tag="KRH39_003764"
     CDS             complement(433..942)
                     /gene="vanZ1"
                     /locus_tag="KRH39_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBG8513630.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDIPSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1262..1651)
                     /locus_tag="KRH39_003765"
     CDS             complement(1262..1651)
                     /locus_tag="KRH39_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8513631.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLNKLEEYFKNLKEEDIDD"
     gene            2094..2663
                     /locus_tag="KRH39_003766"
     CDS             2094..2663
                     /locus_tag="KRH39_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513632.1"
                     /translation="MKLRNPKIKKLLSVALLAALIGIPIGQTANAMEISNNVPSYIEN
                     NPTTIEDLEINDSQLPKPGTVTYIDINKLIKNLKENGIDTTLIENELYYRKAGTNKIV
                     WTKTGFDLYLSKGVALTLAGAGTGVAGLLAGAIPGIGQYLAAAIVGAITMHLGSNITS
                     GKIFKFKKMRYNKGGAYVTKYYLQSVRNQ"
BASE COUNT         1083 a          387 c          341 g         1131 t
ORIGIN      
        1 taacctttat ttttttatta aaatgcatta taataaaaag taaatctaaa tttgatacag
       61 ttttaacaat cattattttc agaaaagtga tactattgaa tatattaaga ataattagag
      121 atggaggaac taagatatga gcgtaattgt aatgtgtagt cgttgtggaa aagaaataga
      181 taaatataga aataaatata taacttatta tgagaaaaat accaatgcag aaatcaaagt
      241 ttgtataaat tgtgcgttag atttattaga aaaaacaaga gaaaatcaag aattgaacat
      301 acaagagttt caagatacaa taattcttta ataatttagg aaagcacctg cataggtgct
      361 ttttattcgg aaataatttt atatgtaatt gtatcttacg ttttatttta agctaaacat
      421 atatacttat ttctataagt ttgcaaaaat aactaatcct aaaaataata caacaataat
      481 tgtcacaatc aatgccaata cattgataat ttttatggta ttattcttaa atattttcga
      541 taatagcata aaaacagcta ttcctatgat tcctcctagt gtgtttccta tcaaatccgt
      601 tatatcactt gcacctagtg caaatatata ttgaagtgtt tcaaatgcca aactagttat
      661 aaaaataggt ataacttttt ttataaagct ccattcttct tttagcatac acacatatat
      721 cccaaatggt acaaatatag ccacatttaa tataatctct gatatatcta ctctattatt
      781 aattataagt gacccagcaa aaggtactaa gtttatgctt ctaagattca tggtttcaag
      841 ggatgggata tcaaattgca acttgaatag tattatccat gttaaaataa ttatgtaaac
      901 tataaataat ccttttgtaa tattatgttt tctagatttc atttattacc tccttactat
      961 attattgtat tattttatta atacaataat atagtaaggt aatttatctg tcaactattt
     1021 cttctaaaat tatattcctt ttataatttc aatatcttta tcatatttac atgatttaag
     1081 ctattgtagt atttcatata tctttaaatt ttaattggta ttattctcta tattttttac
     1141 tatcaaacac taaggaaata atcacttttc tttatataat gttctaccat cacataaaac
     1201 taacattaaa tccaaacgct actctcaatt gtctcaaact aaaaagatac tgatagcttt
     1261 attagtcatc aatatcttct tctttcaaat ttttaaagta ttcttctaat ttgttcaagc
     1321 tcttactatc taaaacttca tcatcatgta atgctgaaat aaaactctta aatgaattgt
     1381 tatgcataaa actaaaaaaa ctctttgtct caacttttaa gtaatctttc tcttttatag
     1441 ctatagtata atgcgtacat ctatctacct tatcagtagt taaaaagccc ttatctacta
     1501 aattttttaa aaatgtcaat gttgtgctct ttttccaatc gtatttcaac ttcatgtctt
     1561 ctatgatatc tcttgaggct aatacatcat ctactcccca aatatacttc attagtttta
     1621 gttctgcatg tggtaattta ttaatcttca ttagtttaca cctcttacca atttatgtat
     1681 aactaaactt tataacaata agtgtatgta atcaattaga ttggaataaa tataaattcc
     1741 aatttattct atataaaaat aattaataaa aaagaagtac tctactttag agagtacttt
     1801 tggattatat attcaaaaat ttatctaaca tgattagagt atgttttatg ctttagcgtg
     1861 ataattttag ttcgttttat tattataata tgtattatat ttcattataa tctataagct
     1921 taaatacagg atatttattt catatactta atttatatta agttttatta tatgtattct
     1981 taagttttac tatatttttt attaatattt aatgtagtta attcttaaga attatattct
     2041 acaataataa tatatatctt tgatatataa ctaatttaaa atggaggtat tttatgaaac
     2101 taagaaatcc aaagattaag aaattgttat cagtagcatt attagcagct cttataggta
     2161 tacctattgg acaaacagcc aatgcaatgg aaatttcaaa taacgtccca agttatatag
     2221 aaaacaatcc aacaacaata gaagacttgg aaatcaatga ctctcaatta cctaaaccag
     2281 gtacagttac atacatagat attaataagc taattaaaaa ccttaaggaa aatggtatcg
     2341 acactactct tattgaaaat gaattatatt atagaaaagc tggaacaaat aaaatagttt
     2401 ggactaagac aggctttgat ttatatcttt ctaaaggagt tgcattaact ttagctggtg
     2461 ctggtacagg ggttgcaggg cttttggctg gtgccatccc tggtatagga cagtatcttg
     2521 cagctgctat agttggagca attacaatgc atcttggttc aaatataaca tctggaaaaa
     2581 tatttaaatt taaaaaaatg cgttataata aaggtggggc ttatgtaacc aaatattact
     2641 tacaatctgt aagaaatcaa taggtgataa aatgattata aaatatctac tttttgcaat
     2701 tatctcagca ctagttttat tcttttgtaa taaaatattt caactaagta aattacacaa
     2761 gttcattata ttgtttgttt cattttttat actaactttt gtagcatcat ttattaatag
     2821 ataaaaaaaa gatggtgggg ttaacctacc atctttttct catttaatat ttgtaatatt
     2881 ttagatactc tttatacttg tgttactgat atactttgat taagaaaagt aaatttaaca
     2941 gg
//
LOCUS       DAEORZ010000263         2939 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEORZ010000263 DAEORZ010000000
VERSION     DAEORZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.269"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(261..2360)
                     /locus_tag="KRH39_003767"
     CDS             complement(261..2360)
                     /locus_tag="KRH39_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBG8513633.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYNGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(2403..2798)
                     /locus_tag="KRH39_003768"
     CDS             complement(2403..2798)
                     /locus_tag="KRH39_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513634.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
BASE COUNT          919 a          469 c          399 g         1152 t
ORIGIN      
        1 gaagaaagat tggttgaaaa tacaattatt ggttgaaaat ataattaata tataaatttt
       61 aaagaaattt acaaaagggg atgtttcaaa atgaaaattt gaatcatctc cttttttgta
      121 aaaaatagaa ataagttgag tttaaaattt gtaaagtatg ctttatataa ttttgattag
      181 aaaaaagcta attttaagac atattttttt aaaaagatga attataaatt ttttatatca
      241 tatgttttat gtaatggaga ttaatggtag ttataagtat tggtgttttc caataaattt
      301 gtcatactgt ttaaacttat tccgacagta gatgcgttgt gtaaaaaggc agatgtactt
      361 ggtctaacaa atccacctac accaagtaaa atcaaaccta aattaaagcc gataataaac
      421 ttagaactta aatcaactct tttcataagc ttattactaa tctgctttaa aacgattaaa
      481 ttattcaaat tatctgctga tatggtaata tctgaaatct ctcttgctat ttcagcacct
      541 tcactcattg caattccgac atctgattct gaaatagcag gagaatcatt gattccatca
      601 ccaatcataa ttactttgcg acctttgagt ttttcacttt taacaaaatt ggctttgtct
      661 tctggtaaga cttctgaata atattcatca acaccaactt tacttgctat agatttagct
      721 gttttttcgc tatctccagt catcatcaca atctttttta ttccgcattc tcttaaattt
      781 gaaattacat attttgcttc ttttcgtaaa gggtcattta tgcaaataac tgctgataat
      841 ttacctgata ttgccataaa taggtgtgaa tactcatctg atagattatt gtacttatcc
      901 tcctcaccgt ctgggatagt acacttctca tcttcaaata caaaatgttt gctacctatt
      961 acaacctttt tatcatcaac ttttgttgaa atgccatgag caataatata ttctacttct
     1021 gtatgcattt ctttgtgact aagcccttgt ttttcagctt cataaactac tgcatttgct
     1081 atggagtgtg ggaagtgttc ttctaagcaa gctgcaagtc ttaacatatc ctccttatta
     1141 taattttgga atgtaattat atctgaaaca gttggttgag cttttgttaa tgtgcctgtc
     1201 ttatcaaata caatagtatc tgcacatgca acaccttcaa gaaattttcc accttttaca
     1261 gtaatgttgt tattgttaca ttctctcatg gctgatagta ctgaaatagg gatagagagt
     1321 tttaatgcac aagagaaatc taccattaat atagagagtg attttatagg gtttctagta
     1381 attgcatagg ttaatatact tcctaaaaag ctgtatggaa ctaatgtatc tgctagatgt
     1441 tcaaatttac tctctacaga agattttagt ttttcagatt cctcaatcat agttactatt
     1501 ttctggaatc tagtagtacc agctttttcc ttgacgcaca taacaatatt gccttgctct
     1561 attacagttc cagcatatat gtaagatcct tcttttttgt taactgcaag ggattcacct
     1621 gtaagtgatg cctgattgac cattgcttct cctgatacta taactccatc caatggaatc
     1681 atatttccca tagttacact tactaaatct ccttccttta tctctgaaat tggaattagg
     1741 atttcagtgt caccttttct taaccatact ttttcaatgt ttagtgacat actttgagcc
     1801 aagtcgtcta tagatttttt tctagtccat tcttctaata gttcaccgat accaagaaga
     1861 aacataactg aaccagccgt attgatatct tttctaaaaa tagaaatacc tatagcagtg
     1921 gcatctaaaa cagaaacttc tattttttta tttagcaggg aagagagacc ttttttaaaa
     1981 tattttatag aatgaagtcc aattaaaaaa aatcttaagg actttggaaa aaatattttg
     2041 gagatacttt tgactagcag tttatttgct aatttgtctc tataataatt gtttaattcc
     2101 ctgctggtat gttcaggaac taggttttct attttgctat tgttaaaact gaatttagtg
     2161 atttctctta ttatgtattc tctttcacca ttgtaataaa tgactgcatc accagttcta
     2221 tcgtaaactt ttgctttggt aataccattt atagaagtta aataatataa gagagtatcg
     2281 gactgcttgt tgtttaattt gccaagttgt aagtcaaagc gtatacgacc tcttatttcg
     2341 tgttttatat aaaaattcaa tttacttcct cctgatatta ttaatcataa taatcattaa
     2401 atctattctg ttgtacaagt actatttttt tcttcaatta cgttatcttt gatttcttca
     2461 catttaatgc tattagaatt agtatcttct acagttttta tagtttcagt tttaagaact
     2521 tcttgttcgc atttaagagt atcttgtaca acgtttacct tagatgtaga taagtcttct
     2581 tgctcgttga tttcttttgc ttcagctaaa atatcttcta cattttcttg aattttcgta
     2641 gttgtttcaa gaacagaatc tttaactttc aatgctcctg ctgttgcacg agtgcaagtt
     2701 tttttaacac aatcacttga aagtatctta acacctacag taccaaaaag tactcctcct
     2761 aaaaatatcc ctgttttttt catttttaaa atattcattt tacattatcc tttcatttag
     2821 ttagattttg tttgtactta tgtataaggt aacaaatgtt aaaaatagag tatgttttac
     2881 aaaattagtt tagagagttt agtaatttta tttgatttat attagtaaat ttacatgaa
//
LOCUS       DAEORZ010000264         2881 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEORZ010000264 DAEORZ010000000
VERSION     DAEORZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.270"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..343)
                     /locus_tag="KRH39_003769"
     CDS             complement(<1..343)
                     /locus_tag="KRH39_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513635.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQY"
     gene            complement(360..764)
                     /locus_tag="KRH39_003770"
     CDS             complement(360..764)
                     /locus_tag="KRH39_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBG8513636.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            1131..1505
                     /locus_tag="KRH39_003771"
     CDS             1131..1505
                     /locus_tag="KRH39_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8513637.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            1502..2365
                     /locus_tag="KRH39_003772"
     CDS             1502..2365
                     /locus_tag="KRH39_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513638.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            2365..>2881
                     /locus_tag="KRH39_003773"
     CDS             2365..>2881
                     /locus_tag="KRH39_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG8513639.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPISRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENIS"
BASE COUNT         1066 a          340 c          383 g         1092 t
ORIGIN      
        1 tatattgata aatagcaata ttattaaaca cttttaatat gtttgaattt acaaaattgt
       61 aaatttgatt attgttatac agtgatattt gttcgtatat atattcccta aataaagggt
      121 gaatgttttg cttaagtttt aaagtaactt cagctggtat ttcaaaataa agcttccagt
      181 acttagagtt atttttttct aaaataaaag ctactttgtt caaactggta gaactaatct
      241 cctttagtgt acaattaaaa ttatagtgtc tcaaactagt tttttcattc tcaaatacat
      301 agtcaaacca ctgtagaaca tttctatcaa gttttaaact catataaaaa ccttcttcct
      361 tatttactaa ccttattagg tctttttatc tgattatatt ctgctaataa actttcttta
      421 tcagctctac tacctttata tgctatatgt ggatttatca tatactcacc atttttcact
      481 ctttgtaaaa aatccatttt tttcagctcc gtaaacaatg aagataaaga agtttttgga
      541 tattcaaatt cacaagataa ttcatataat gtttttctaa tcttattatt tttatccata
      601 gagtttatta attttaatgg aattaaatcc ctcttagtat acattccttc tactacttta
      661 atataattct taaaacaaac attcatatac ttacttttat tcataactaa acttttgctc
      721 tctgtatatt cctgatttaa atcctttttt aatgtcttat ccaaattaaa tatctcttct
      781 cactctcctt atctctaatt gataatataa atatattaca aaaaaatatg tatgtcaaaa
      841 ctgaaaacgc aaaaagttca ccttataagt aaacttttat ttagctataa gctaaacttt
      901 attatttttt taatttagta tttccaattt attggcagga tttttgaaca atatttataa
      961 attttttaaa aaaatttaaa tctatttaat atggcagact ttcataaaat acattacatt
     1021 tattaaatgt ataatattgg taattagttt tttctaaaat cagttgacat attatatata
     1081 tatttatata atgtgtatat actatatata caatttaagg aggtgtctta ttgaatatag
     1141 ttattagtaa ttcgagtggg aagccaatat atgaacagat tactacacaa attaaaagta
     1201 tgattataag tggagagtta ccagaaggct cagctcttcc aagtatgaga ctattggcaa
     1261 aggaacttag aataagtgta attactacaa aacgagcata tagcgattta gaacgagacg
     1321 gatttattga gacagttact ggtaaaggca gttttgtatc tagtaaaaac atggattttg
     1381 tcaaagaaga acaatttcgt ttaattgaag aatacctaca aaaagcagtt gatacagcaa
     1441 aaagtagtga tattacatat gacgaattaa aagatatttt atttttatta tataaaggag
     1501 aatgataagt atggatgcaa taactataaa aaatttaaat aaaacttata aagattttag
     1561 cctacaagat atatcttttt cagtaccaaa agggagtgta atgggatttg taggtgaaaa
     1621 tggtgcagga aaaacaacta cattaaaagc aattttaaat ttaatatctt atgatagtgg
     1681 aaatatagaa atctttggtc ttgatagtaa aaaaaatgaa aaagaaatta aagagcaaat
     1741 tggggttgta tttgagggaa gcaactttca tgaaaattta aacactgacc atgtttcaaa
     1801 aatcatgagt aaaatctata aaaattggaa tgacacctta tttaaagact acttaaaaaa
     1861 attgagagta ccagacaata aattaattaa ggagttttca aaggggaata agatgaaact
     1921 tagtattgct gtagccttat ctcataaacc aaaattatta atactagatg aggcaactag
     1981 ttcacttgac cctatcgttc gtgaagaaat actagatata tttttagatt ttatacaaga
     2041 tgaagaacac tctattatct tatcttcaca tattacaagt gatttagata agatagcaga
     2101 ctatattaca tttatacata aaggaaaaat tgtatttagt gaaaataagg atgaattaat
     2161 tgatactatg ggagttttaa aatgtaaacc tagtgatttt gataaccttt caagagagga
     2221 ttattcatat tatcgtaaaa gccaatttgg atatgaagta cttcttaaag ataagcacag
     2281 atttatctca agacatccaa actgtattgt tgataatacg tctatagaag aaattatgtt
     2341 attttatgtg agaggtgata aataatgaaa ggtctaattt taaaagattt acttaatttg
     2401 aaaggaaata taaagtttat acttttattc atcattatgt ttggctttat gtcttcttta
     2461 ggagatggaa atgttaataa ctttataggt gttattatag ttttatgtac tacaatgatt
     2521 gtttcaacat tttcatatga tgatttaaat aaatgggata gttatgtact tacaatgcct
     2581 attagtcgca atgatattgt attaagtaaa tatttaacta tgttaatatt tagctttata
     2641 ggtgttcttg tttcacttat tgttagtgta actattggtt attttaaaaa tacacttata
     2701 ttaaatgaaa cactacttat taatgcacta atcttatcaa tttcagtatg ttttggtagc
     2761 cttattctac cacttattta taaatttggt acagaaagag ctcgcttatt aatgatttta
     2821 tgctttttag ttccaacact agctttatta gtttttaaaa gtattttaga aaatattagt
     2881 t
//
LOCUS       DAEORZ010000265         2877 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEORZ010000265 DAEORZ010000000
VERSION     DAEORZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.271"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            228..1109
                     /locus_tag="KRH39_003774"
     CDS             228..1109
                     /locus_tag="KRH39_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG8513640.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(1170..2378)
                     /locus_tag="KRH39_003775"
     CDS             complement(1170..2378)
                     /locus_tag="KRH39_003775"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBG8513641.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(2451..2703)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          960 a          474 c          341 g         1102 t
ORIGIN      
        1 acaccttttt aataaatttt tatatttttt atattttttt acctatacta aattttttta
       61 tagatatttt taatatatat gatactttaa tattatgcaa ataatgtcat ataagttaca
      121 ctttagttat cttagttaca actttgtcgg tttacttttt ctactttctt cgatataatt
      181 ataatatatt tttgatacaa ttcgacaaaa gggggaatgc tgtaattatg gtaaaaaaaa
      241 gaaaaaagaa aactgttaca cttactatac ttgttgtaat agttattggt attttttcta
      301 tggcatgggc ggacatgtct agaaaaaaag aaatgaaaga aactcaagaa agacttaaag
      361 ctgaaagatt agaaaaagaa agaattgaaa aagaaaaaaa tgaacctatt attggtgcaa
      421 aaaaagaagc tgtaaaagaa tatggatatg acgcaaaaat agtttgggac aaacttaaca
      481 aatacgacta ttcaaacaat ggagaaaaga tagtctttct tacatttgat gatggaccat
      541 ctactactaa tactccacaa gttctagata tcttgaaaag acatggtgtt agaggaactt
      601 tctttattaa gggtgattct cttgaaagaa aaggagctaa tgaaatttta aaaagaacat
      661 ttgacgaagg aaatgcaata gcccatcact cttatactca tgactataaa aaactatatc
      721 ctaacagaag cctaaatctc gatacattcg taaatgaact taacaaaact gatgaagcta
      781 tgaaaaaagt cttaggaaag aatttttcct caaatgtagt tagatgccca ggcgggtata
      841 tgtcatggaa aaatatggaa cctctaggaa actacttaaa agagaaaaac atggcatcaa
      901 ttgattggaa cgctttaaat gctgatgcag aaggtaaaaa gaaaaatgca caagaattat
      961 ttgaacatgc aaagaaatct tctgaaggaa aagaaatggt tgttctttta atgcatgata
     1021 cttatggaaa acaagaaaca gtaaatgcat tggaccaaat tataacatat tttaaagata
     1081 atggttatca atttaaaatt ttaatataat aaaaaagcaa gtttattgaa tgtacatttt
     1141 atactcaata gacttgcttt tactttactc tattttacta gctcgatttt tacaaatttt
     1201 ttctttccta tttgaacaac taattcactc tcttttacaa tagtgcttaa atcctctact
     1261 ttttcaccat ttacctttac tccaccttga gaagctaatc ttctaacttc actcttactc
     1321 ttaacaatct cattagaaac tagtacttcg attaagtcaa atccatcttc cttaacttga
     1381 attgtttgta tatcttcagg tatttgacct ttttggaata cagatttaaa tctttcttca
     1441 gcttctttag cagattcttc tccatggtat aatgttacta tttctcttgc taagttcatc
     1501 tttatatctc ttggatttac tgaaccctct ttcaactgag attctatttt attaacttca
     1561 tcaggatgaa catctgtcac taaattgtag tatttaatta taagttcatc aggaatttcc
     1621 atagattttt ggtacattat tcccgcttct tcatctattc caatatagtt acctaaactc
     1681 ttactcattt tttctttacc atctaatcct tctattaaag gcatcattat tactatttga
     1741 gactccatac caaattctct ttgtaatgat cttcccatta gtaaattgaa tctttggtca
     1801 gttccaccaa gttctatatc agcttctaat gctatagagt catatgcttg cattaatggg
     1861 tagaagaact catgtactga tattggcatt tgtccttcat atctcttttt aaaatcttct
     1921 ctttctaaca ttctcgcaac agtaatagtt gctgctaatt ttattacatc ttcaaaattt
     1981 aacttagcta accactcgct attaaatctt acaatagttt tttctttatc taaaactttg
     2041 aaaatttgtt cttcataagt ttttgcattt gcaagaactt gctctgttgt taaagctttt
     2101 ctagctttag attttccagt tggatctcca atttttcctg taaaatctcc aattattatt
     2161 actatttgat gacctaaatc ttgtaattgt ttcatctttc taagaactac tgtatgccct
     2221 aaatgtatat ctggagcact tgggtctaga cccaatttta ctatcatagg ctttccttct
     2281 ttttctgatt taataagctt ttcccttaac tctttctcat ctattaaatc atctacacct
     2341 ttcataatta ttcgcatttg ctcgtctatc gatttcattt ttcattcctc ctaaaatatt
     2401 tatataatct ataattttta tttttataat aaaaaaagct ttcatcctct taaaaggacg
     2461 aaagcattgc ttacgtgtta ccaccttagt tcatcaatat ctcacgatat taatctctta
     2521 gagtacaatt acatatactc gtgtactata acgtgtacta atccgatatc tcttaatggt
     2581 tgttatcttt cagagatagt gctctgagat gtattcaaac taaatcaatt tattccattt
     2641 caccaaactg gaactctctg ttaaataaaa tatagcccta cttcttctct tcatagcatt
     2701 tatatattca gattatttca tcaattacaa gaaatttttc ttaacttact cataatatat
     2761 tattattttt atattttgtc aacctttttt attattatat acatgttttt ttacattata
     2821 ctactttttt atatttttta ccaataccca aagtttattt ttcaatcaaa agattta
//
LOCUS       DAEORZ010000266         2877 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEORZ010000266 DAEORZ010000000
VERSION     DAEORZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.272"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            353..1771
                     /locus_tag="KRH39_003776"
     CDS             353..1771
                     /locus_tag="KRH39_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBG8513642.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GIFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIVPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            1847..2659
                     /locus_tag="KRH39_003777"
     CDS             1847..2659
                     /locus_tag="KRH39_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8513643.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDEVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVIGNPRESYIDGIWNKENEEDWLTEVQIPVKRIL"
BASE COUNT         1082 a          297 c          490 g         1008 t
ORIGIN      
        1 agatttcata tctagagtac ctaacattac atgttagttt cttgttactt atctaattac
       61 tttatatttc atagttatat gtaaaattac tataaaaaat gaaatatatt aaacaatcaa
      121 ctgaaaaatg aaatgttaat aaaaaaaaat tcaaattata aatattaaat gaatataatt
      181 aaaacaaata tataaattta agtataacac tcacatttta atgcattgtg aaatttttat
      241 tgaactttat gtaccataac ttataaaatt tatgtcatgt tatatttgaa taagtaataa
      301 aattgtagat gtaaattaat ctacaaaatt tatatgaaat ggagttgatg ctatgacaga
      361 attaatgatt ataagtgggt tagtactatt gatttgtata acttctagca aggtattgta
      421 taaatttgga gtaccaatat tattgatttt tatcttacta gggatgttat ttgggtctga
      481 tggaatggta ggaatctatt ttgataacta tgaattaaca aaacaattat gttctattgg
      541 actaatattt ataatgtttt ttggagggtt tggaacaaac tggaatatgg ctaaacctgt
      601 tgctatacct tctatattaa tgtcttcact gggtgtaata attacggctg gtgtcacagg
      661 tatattttgc cacatggtaa taggaactag tttacttgaa ggtttattaa ttggggcaat
      721 tgcagcttca acagatgctg catctgtgtt tgcggtgttg cgttctcaaa aactaaactt
      781 aaagggttcg cttgcctcat tattagaagt tgaaagtgga agtaatgacc caatagcgta
      841 tatgttgaca ctaattcttt tgacattgat gaataattct ggggtagaac ttattgtacc
      901 aatggtaata aaacaaatag cttttgggtt agctatagga tttatactag caaaattatc
      961 tgtttatatt ttaaggcatt caaacttcga gatagaagga ttttatacta tatttataac
     1021 agctattgca atactttctt atgcatttag cgagtatctt ggaggaaatg gatatttaag
     1081 tgtttacatt tcaggtatta ttataggaaa tagtagaata cctcttaaaa aaagtttggt
     1141 tcatttcttt gatggaattt catggattat gcaaataatg ttattcttta tgttaggatt
     1201 gttatctttc ccttctaagt taccaaatgt atttggtata gcaatggcca tatctatatt
     1261 catgatagtt attgcaagac cattagcaac atttatagtt ctagcaaaat ttaaattctc
     1321 aaataaagaa aaattattca tatcgtgggt aggccttaga ggtgcggctt cagtagtttt
     1381 tgcaatttat gctgttactc aaggagtagc catagagaat gacatattcc atataatatt
     1441 ttttatagcg ttgctttcag taggaataca aggttcactg ataccaactg tagccagaaa
     1501 gctggattta gttgatgata atacacctgt attaaaaaca tttaatgatt atgatggaga
     1561 aattagttct aaacttatcg aagtaaatgt tgatgagggt aataaatggg ctaataagag
     1621 tataatagat tctaatatac cagacgatat attaatagtt atgataaaaa gaaaggaaca
     1681 ggttttaata cctaaaggtt caaccattat aaaaacagga gatattttgg tattgagtgg
     1741 agatagaatt gaagaattaa tacaattata atttattttg tattgacttt aacctaaggg
     1801 gaaagtttaa acttaaatta atcaaatcta atgcgcaggt gatgagatgt tttctatagg
     1861 aatgttttct aaaataaata aaataactac aaagacatta aggtattatg aagatatagg
     1921 gttgttaaaa ccagaatatg tagatgaatt tacaaaatat aggtattata caacagagca
     1981 attacctaaa ttacatcaga taataacttt aaaacaaatg ggtttatcat tagctgagat
     2041 aaaaaaagct gtagaaaatc ctgaagaagt tgaaaatatt ttaatgaata aagaaaaaga
     2101 aatgttggat gtaattaaaa gtgaagaata taaaataatt aaactacgta attaccttat
     2161 gaatttgaaa ggagaagtca atatgaatga tgtaattatt aagtctttac caaaagttaa
     2221 agttgcttct atgagaaaag ttttaaaggg atatgatgaa cttttcaatc tatttccaaa
     2281 tgtaatggct gaagaaatga tgaaaacagg gtgtacatgt gcagttccag attattgttt
     2341 taatatatat catgatggtg aatatagaga gactgatatg gatgttgaaa tgtgtcaagc
     2401 tataaatgat ttaaaggaag atacggatat attaaaattt aaagaattag atgaagttga
     2461 aaatgcagtt tgtatattac acaatggtcc atattcaaca ttaggaaaaa cttacgcaga
     2521 tgcatttaaa tggatagagg acaataaata taaagtgatt ggaaatccta gagaatctta
     2581 tatagatggt atatggaata aagagaatga agaagattgg ttgacagaag ttcagatacc
     2641 agtgaaacgc atattataat ctgtctacaa agtataaata agaataaaaa aggtggtgtc
     2701 tcaaagtaaa ctttttagtt tatgagacac tctttttcat atgaaatcaa gtatattttt
     2761 ataattccaa ttatgaaaaa aggcttatct ctattttggg agagcctctt atttttttat
     2821 tataatagtt ttctaaagat gctctatcta taaattttta tcaaagattt tataaat
//
LOCUS       DAEORZ010000267         2856 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEORZ010000267 DAEORZ010000000
VERSION     DAEORZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.273"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            218..421
                     /locus_tag="KRH39_003778"
     CDS             218..421
                     /locus_tag="KRH39_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8513644.1"
                     /translation="MISYAPLWKLLIDKKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            585..1739
                     /locus_tag="KRH39_003779"
     CDS             585..1739
                     /locus_tag="KRH39_003779"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBG8513645.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1752..2756
                     /gene="rseP"
                     /locus_tag="KRH39_003780"
     CDS             1752..2756
                     /gene="rseP"
                     /locus_tag="KRH39_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBG8513646.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
BASE COUNT         1120 a          286 c          528 g          922 t
ORIGIN      
        1 tagtaaatat tttttatata gttatgtttt agtaaaataa tgagataaat atatataaag
       61 tttaattttt gaacttatgc ttttgtttta ttttaacata tttatattta ataaactata
      121 attgaaatct atttagataa ataattattt atctaaatag atgaaaattt tccaaaatag
      181 ataaaatgtg atagaatata ttttgcgagg tgataacatg atttcatacg ctccattatg
      241 gaaattgtta attgacaaaa aaataaagaa aatggaattt gtaaatatat caggtataag
      301 tatttctgtt ttaggcagac taggaaatga taaatctgtt tctatggata caatggagaa
      361 gatatgttta gcactagact gtaaaataga agatgttgtg gaaataaaaa agggcattta
      421 ataatactag aatattttat aatattccac aaaatacaaa aaataataga ttaattttaa
      481 tctgtggaat aatatatgaa atttctatga atagtaaaaa taattgtttt agtagtgtta
      541 taatgttttg acagggtata tttattatta gaaaggatta ttatatgaaa aagatatcaa
      601 ttttaggttc tacaggctct ataggtaaac aaactttgga tgtagttaga gataatagag
      661 ataagtttga gatagttgca atatcagcta acagtaatat tgaattattg ctagaacaga
      721 tagtagagtt taaacctaaa tatgtaacag tttttgagga aaataaagca ttgaaattaa
      781 aagaaatgtt gcctaaaaat atagaaatag aagttttagc aggaatggaa ggattaaaaa
      841 taatatcatc acttgatgaa gtggatgtcc ttttaactgc tgtagtaggt atgataggat
      901 tagtcccaac tctttgtgct ataaagaagg gaatagacat agcattagcg aataaagaaa
      961 cgttggtaac tgctggtgaa cttgtaatga aagaagcaga aaaatacaat gtaaatattc
     1021 ttcctgttga tagtgaacat agtgctatat ttcaatgtct aaatggagaa aataaaaaaa
     1081 atatagaaaa gataatactt acagcatctg gtggtccatt tagaggaaag aaaaagggag
     1141 aacttgcaaa tataactaaa aatgaagctt tgaagcatcc aaattggagt atgggaagaa
     1201 aaataagtat tgattcttca acgcttatga ataaaggact tgaagtgata gaagctagat
     1261 ggttatttgg agtagaacaa gaaaatatag atgtagtagt tcacccacaa agcataattc
     1321 attcaatggt gcagtataca gatagctcaa taattgctca attaggatgt ccagatatga
     1381 gactaccaat acagtatgct cttacatatc cagatagaat ggaaagtagc tttgaaagaa
     1441 tgaatttttc aaaatttagt actttaactt ttgaagaacc agatttagaa acttttccat
     1501 gtttaaaatt ggcatatgag tgtttaaaaa tgggaggaac ttattcttct gttttaaatt
     1561 cagcaaatga agttttagta agtgaatttt tagaggataa aattggtttc tatgacatac
     1621 catattacat agagaagact ttagaagttc atagtagcat aagtgaacca acattagaag
     1681 aaatcttaga aacagataga tggagtagag cttatgttgc aaatctgata aaaaaatagg
     1741 aaggtgaatt tatgactata atagccgcat taatactatt tagtataatt gtattaatac
     1801 atgagttagg acattttata tttgcaaaaa gaagtggtat caaagtaaat gagttttcta
     1861 taggtatggg gcctaagata tatagtgtaa aaaaagatac agagtattct ataagagcac
     1921 ttccaattgg tggatatgtc agcatggaag gtgaagatga agaacaaata agtccaaatt
     1981 cttttggaaa caagtctata ttacaaagat ttagtactat tgtagcagga ccaatattca
     2041 atataatatt agcagcaata cttttagttc cagtattttt gtatataggt tctccaacga
     2101 ctaaattagg aaagataatg ccagatactc ctgcacaggc tgtagggctt caagtaggag
     2161 ataagataaa taaaattaat ggtaattctg ttaagacttg ggatgaagtt gcaaatataa
     2221 taaatacttc ttctggtgga gaattaaaac ttagtataac tagagatgga agtgataaag
     2281 ttgtaaatgt aacacctaaa aataacaatg gaaagtatga aatagggatt caaccacaaa
     2341 gagaaaaaga ctttttaggg tcaatagtaa atgcatgtaa aactactgta gatatgacaa
     2401 aacagatgct aacatttcta ggtcaaatga ttacaggacg tgtaccagga gggattggca
     2461 atgctgtagc aggaccagta ggtgttattg gtatggtatc agatgctgct cgtacaggca
     2521 tcataaatgt agtgtattta gcagctgtca taagcttaaa tttaggtata gtaaatctat
     2581 taccaatacc agcacttgat ggatggagaa tacttatgct gttactagaa gctgttagag
     2641 gtggtaaaaa actcgaccca aataaagaag gcatgataaa tgttgttggt tttggagctt
     2701 taatgttatt tatgcttttt atcacatata aagatatact aagattattt caatgattta
     2761 gtttttagag attttaagaa aataggctat gtagttttaa ataagaataa attaattttt
     2821 atatatgaaa tttattctta tttaactaga tagctt
//
LOCUS       DAEORZ010000268         2831 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEORZ010000268 DAEORZ010000000
VERSION     DAEORZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.274"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            60..2417
                     /locus_tag="KRH39_003781"
     CDS             60..2417
                     /locus_tag="KRH39_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBG8513647.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2503..2820)
                     /locus_tag="KRH39_003782"
     CDS             complement(2503..2820)
                     /locus_tag="KRH39_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBG8513648.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1053 a          321 c          472 g          985 t
ORIGIN      
        1 ttttataata tatttgtaga tttgagtata aaaaagtgct aagttgaaag gagtaaaata
       61 tggataaggt taatttagaa aggtatagtt gtgatataaa tgttggttta aataaaaatc
      121 aacttcaaag tagaatagat gataatttaa ttaatgcatt tgataatgga aaaaccaaaa
      181 catataaaca aatttttaaa gacaatatat ttacactttt taatctcata aatattgttt
      241 tagtctgttt attagtttca gttggttctt ttaaaaatac cttatttata tttattgctg
      301 taattaatac tattatagga gtaatacaag agataagagc taaaagatta ttggacaatc
      361 tatctgttat agtttcaaat aaggtatctg tcataagaga aaaagagaaa aaagagattg
      421 atgtacaaga attagtatta gatgatatta tgattttaaa aacaggtaat caaatttgtg
      481 cagattcaaa agttttaaat ggaaaattag aggtaaatga gtctttggtt acaggtgaat
      541 ctgatattat tataaaaaat aaaggcgatt ttttatattc tggaagtttt gtagtttctg
      601 gagaagcttt tgtaagagtt gaaaatatag gaaaagataa ctatgcaaat aagatagcta
      661 atgatgcaaa gatttctaaa aaacataatt ctcaacttag aaattcatta aatgcaatat
      721 taaaaattgt aggtataata ataattcctc taggaattat attgtttgca aaacaacatt
      781 ttggaaatgg agattctata gctacatctg tagtaggtac agtggcagct gtaaatggta
      841 tgataccaga gggattaaca ttacttacga gcattgctct agctgtagga actataaaac
      901 ttgctagtca taaaacttta gtgcaagaac tgtactgtgt agagactctt gctagagttg
      961 atacactatg tcttgataag acaggtacaa ttacagaagg taaaatgcaa gttgatgata
     1021 tagtaatgtt ggaagaagtg gatattaatg aaataatggg gaatatatgt aattctctca
     1081 aagatgataa tgcaacacta aatgcaataa gagataagta taatgtttta gatacgtata
     1141 aagctaagaa tttaatacca ttttcttcag aaagaaaata cagtggggtt acctttgaaa
     1201 ataaaggtac atatttatta ggtgcgtttg aatttatatt taaggaaaaa aataaaaaac
     1261 ttaggataga agttgaaaga tattctaaaa aaggaaacag agtaatagtt ttagctcata
     1321 gtgattctta tatggaagat aataatattc ctgaagatat aaagccacta gcttttatat
     1381 tgatattgga taaaattaga gatgaagcta aggaaacttt agaatttttt tacaatgaag
     1441 gtgtagatat aaaaattata tctggtgaca atcctataac agtaagtgag gtagctagaa
     1501 aagcaggtct taaaacagct agtaatttta ttgatgcaac aacacttaaa aatgatagtg
     1561 atatatacaa agcagttgat aaatacagtg tttttggaag ggtaagtcca caacagaaaa
     1621 aacaaatgat tttagcatta aaaaaacaaa atcatacagt agctatgact ggagatggtg
     1681 taaatgatgt gctagcactt aaagaagcgg attgtagcat tgccatggca tctggaagtg
     1741 atgttactaa gaatgtgtct aatttagtct tattagattc aaactttgca tcaatgccaa
     1801 aggttgttat ggagggaaga aaagtaatta ataacattca aagagcatct tctttgtttt
     1861 tagtaaagac aattttttca ttcttcttgt cgttattaac attattcttc ttcagtagac
     1921 agtatccatt tgttcctata caattatcac tcattggggc tgtaacagtg ggtataccat
     1981 cattcttctt agctcttgaa gcaaacaaaa gtagaatttc aggaaatttt ttactcaata
     2041 tattaaaaaa tgctttacca ggagcactat gtgtgacagt aaatgtaatt attgtttaca
     2101 atatagttga atatttaggt tatagttcaa gtgtattttc cacaatgtgt gctatattaa
     2161 ctggtgtatg tggtctttta atattaagac aacaatgttt gccatttaat aaagaaagat
     2221 tatttttaat agtaagtatg actattgcat ttgttattgg gattctattt ttaggtggat
     2281 tattctcatt tgtagcattg agtagagaat taattctcat aactatggca ttagcaatat
     2341 taatgccaat tatgataaaa gttatgttat ttgtcttaga tgagatatta gagacaattg
     2401 ttccagattt aaattaattt taaaatgata taaataagag cagttttagt tgaactgctc
     2461 tttttagatg tgtttaattt tatgtaagaa tatgtatttt tattaatcag ctatatatga
     2521 gcaacagtag tcaacaactt cattaacctt taagtcaaaa cttgaatcac taggaacatt
     2581 aaatttttga ccttctttat aagttacaaa ctcattgctt ccaggcagtt ttacatccat
     2641 acttccagca agcatctcca ttatttcttc ttctctagta gaaaaagtgt attctcctgg
     2701 caacattaat cctaaagttt ttctttctcc attaggcagt attattactc tgctacttac
     2761 cttaccatca aaataaacgt ttgccttctt tactgcagta acattcttaa attcactcat
     2821 tagataaaac c
//
LOCUS       DAEORZ010000269         2775 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEORZ010000269 DAEORZ010000000
VERSION     DAEORZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.275"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            172..549
                     /locus_tag="KRH39_003783"
     CDS             172..549
                     /locus_tag="KRH39_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8513649.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDSLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVDKGYLARDIIKFQSHYKIIIDNKEYYAFKKKVLKSSKSRKIMRSLT
                     TTHKSISKEKLDALEEYYRNLEE"
     gene            660..1046
                     /locus_tag="KRH39_003784"
     CDS             660..1046
                     /locus_tag="KRH39_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8513650.1"
                     /translation="MKISKLPEAELKVMRYIWESDKVLTSREITEAMEQKYEWKDTTT
                     FTVLKRLQRREFLETEKIDKRTHYNVLVKEKQYQRFETREFIKNIHKNSIKSLISALH
                     KDDEKVDEKKLDELEEYFNNLEENEK"
     gene            complement(1244..2089)
                     /locus_tag="KRH39_003785"
                     /pseudo
     CDS             complement(1244..2089)
                     /locus_tag="KRH39_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
     gene            complement(2280..2693)
                     /locus_tag="KRH39_003786"
     CDS             complement(2280..2693)
                     /locus_tag="KRH39_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8513651.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKVAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYFRKIL"
BASE COUNT         1115 a          366 c          368 g          926 t
ORIGIN      
        1 tgttcaacaa gattgtttga aaattaaata aatttgtaga tgtgaataat attttttata
       61 tataaaaaca aactaaaaca gtagaatatt tggtaaaaat tacctctagt ttttgtccta
      121 aaaagctcct attattaatt taagttagaa gaattaagga agtgacataa attgaaagaa
      181 gaaatactaa tagaaaaatt attaagggca gaattaatag taatggagta tttatggaaa
      241 aaagattctc taatgcctaa aaaagaaatt gtaaaagaaa taaaacaaat atatggatgg
      301 cataaaagta caataaaaat actactaaaa aggctagttg acaaaggtta tttggctaga
      361 gatattataa aatttcaatc tcattataaa ataataatag ataacaaaga atattatgct
      421 tttaagaaaa aagtgttaaa atctagcaaa agcagaaaaa taatgcgttc tcttactact
      481 acacataagt ctataagtaa agaaaaacta gacgcattag aagaatatta tagaaattta
      541 gaagaataat ttcgagatgg aaaaaattgg atggtaatat ttagtgtgat tctcttgttt
      601 ggaaaacttt attgagatag aatttaacat atacataatt caataaaggg gtgcaaatta
      661 tgaaaattag taaattacca gaagcggaat taaaagttat gagatatatt tgggaatcag
      721 acaaagtatt aacatcaaga gaaatcactg aggctatgga gcaaaaatat gaatggaaag
      781 atacaactac atttacagta ttaaaaagat tacaaagaag agagttttta gaaacagaga
      841 aaatagataa gcgtacacac tataatgtat tagtaaaaga aaaacaatat cagagatttg
      901 aaacaagaga atttataaaa aatatacata aaaattcaat taaaagtttg atttcagcat
      961 tacataaaga tgatgagaaa gtagatgaaa aaaaattaga tgaactagaa gagtatttca
     1021 ataatttaga agaaaacgag aaataaagag tattgtttta caaagaaaaa atgggtgtca
     1081 aattgttttt gccacccatt tgccaccaaa ctggtttaat aaaataaaat acaaataaag
     1141 aagcataaag aaaataaaat aaagaaatcg ttttagatga attgaaaata gatgtattca
     1201 taaaaacata aataaatata aataagcata aagacaatat gagaatttca agagtaaatt
     1261 cagctctttc aggtaccaca tttgatgaag atggatagaa attcatcttt cctacagtag
     1321 caaccaaatc agcagtcgtt ttagctactt tttcgatagc taatacacat tcacaggcac
     1381 aacatccagc atcaagtctc atattcattg gaagtgtacc agcatgtcca gacttaccat
     1441 ttactgatat cttaaaactt ttaaatcctg taatacttga tacaattcca gcaggaagag
     1501 attcattttc taaaacagga ccctgctcga tatgaaattc taaatatgca tctacatctt
     1561 ttctaggatg caaatttgtt aagttttttg tattaaatcc aaactttgtt acagcttctt
     1621 caagtgtaat tccattttca tcaacagatt taagaagctc ctctttaaaa gtaccagcaa
     1681 aaactttact tcccattaaa gaagtttcaa atctagttcc ttcctcttca gcgaaagcaa
     1741 ttagcttaat attgaaagga taagacctat tattgtgttt tgcttcttca agaccaacta
     1801 gaggcactat tatacctaac ataccatcaa atatacctcc acactttaca ctatcttgat
     1861 gagatgcaac tactaatgtg ggagcatttg gaaagcttga tttatatgta cctataacgt
     1921 ttccaatgtc atcaatttca atatcaagat ttaaaccttc catccatgaa ctaattaaat
     1981 caatagattt tttatgttct tgagtcaaga taagtcttgt aagaccattt gttgttgaac
     2041 ttatttttcc taatgtttgt agataatcca tacatttttt tcctaacata tttacctcct
     2101 aaaaatataa tttttattta agctaagatt agcttttata cttataattt ttgaactttc
     2161 aaataatttt gtatgtatag taaaatacta ttttagtatt aataatgata taaaaatata
     2221 aagtctgtag acgaagagca gaagttttaa gttaaaaata taaaatatga tttttattat
     2281 tatagtattt ttctaaaata atatctcttt ccagttagag gataattttc aagtgaaaat
     2341 actttatggt atccaaattt ctcataaaat ttaggtgctt gaaaactgaa tgtatctaaa
     2401 aaaacatatt tacaacctcg ttctttagca actttttcag ccttatataa aagttgactt
     2461 ccagtacctt tatatctaag cttctcacta acccataagt attttataga aagccaatta
     2521 ccatgagtaa cacctataaa acctgcaaca attttacctg ctttatctcg aatatatatt
     2581 cctaaatcca ctgggttttt atcttcaagt ctagcaagat tatattttaa taatccttga
     2641 aaaatagtat cttcatcctg tttttctatt ttttctgtaa tttctaattc catatttgaa
     2701 ctttctcctt gtataatctt ttgtatattg gtatttttta ataaaatcag tagaaagaat
     2761 atagtttaac attat
//
LOCUS       DAEORZ010000270         2748 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEORZ010000270 DAEORZ010000000
VERSION     DAEORZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.276"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            374..2183
                     /locus_tag="KRH39_003787"
                     /pseudo
     CDS             374..2183
                     /locus_tag="KRH39_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
     gene            complement(2271..>2748)
                     /locus_tag="KRH39_003788"
     CDS             complement(2271..>2748)
                     /locus_tag="KRH39_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513652.1"
                     /translation="KKDNSNFNYTNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGT
                     EIFFTWEEIHCWDEKVATEKTFINGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYE
                     EKGLKIERHALGSEDGEYVELTNDSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1040 a          282 c          399 g         1027 t
ORIGIN      
        1 aaattgtaga taaatagtat tttaattatt tattaaatat agattatgta tatgcaaaat
       61 ttaaaaagag acataaatac attatacaaa tatattattg gggggatatt ttataagaaa
      121 agaagtgatg cagtaaatta attgagcaat ttaaaaatga tttaatgata gtgttgatga
      181 atgtctaaaa agaaatttta gaaaaacgta tattgttgtt tgtgttggag gtgatgaatt
      241 tgtagaattt atagagtata aatgattaaa ctatttaaga taaatacata tcactgtaaa
      301 atattgataa tcatttttta taagagattg attatctgca aattatatat atattacaaa
      361 tgaggtaaca ataatgaaga aaaaatatga ttttttaatt tattctgcta tttggctagt
      421 tattattatt ctttttatat tttctctata tcatagtata gagcatatta aaattataag
      481 tcatactggt actattcgca gtgaaactca aaaggttgtt aagcaagaat tgaataatga
      541 aagaaatgat gacttgataa aacgactgga taatattctg ataaagctaa gaacaggtaa
      601 tggagaaaat ggatttcaaa gatgtgataa taaagaattt caacaaaaat taaatcaaat
      661 ggattctata tgggaatcta tgaagaaaga aattataaaa gttagaaatg gtgcttaggt
      721 gataaattat ataaattgag cgaagagtat tctgtgttgt ctaatcaaat tgtttttata
      781 tcggaaaaac actctaatgc aaaattgtat tcttttgcta ctgcactatt tatttatctt
      841 atattctcaa caataagtct tttaatttgg gaatattata ataaaaaaag gtttaaaaga
      901 atattttata ctgataattt aacaaaaata aaaaatcaag ttgcatttga aaacagagcg
      961 attgaaattt tgcataatgc atctaacaaa gagtatgtac tgctaaatat tgatattgac
     1021 aattttaaat atattaacga tacacatgga tatgaatatg gagataaagt tctaataata
     1081 gttgcaactg cactttctaa aacttttagt atcaaagaaa cttgtgcaag aattggttca
     1141 gataattttg taatacttgc aaaatataga gattctcttt tagaggatat tcgggaaatg
     1201 ctaactaatg ctataatctc tgaattagat atgaatgtaa ctcaaactat atcttattgt
     1261 ataggagcgt acttagttga aattgataat ttaggttata agtctatcaa ttctataatg
     1321 gataaggcaa atattgcaca caaggtaagt aaaacaagag gaatttcctc tacagtatgg
     1381 tataatgaaa atctgttaaa acagttacaa atggaaaata gtatttataa ttatatgtat
     1441 aaagcgatag aaaatgaaga atttcatatg tatttgcaac ctaaatttca aatatcttct
     1501 ttaaatgttg tgagcgcaga agctcttgta agatggtttt ctccagaact tggtttttta
     1561 tcaccagatg aatttattcc attgtttgaa aaaagtggat ttattattga attggacttt
     1621 tatatgttaa aaaaagcatg ctcatttgta aaaaaaacct ttatgaaaaa aatcaatata
     1681 catatccgat tgcagttaat ttttcaaggg ttactattta ccaaaatagc ttttatcaaa
     1741 ggtttttaga tactgttaaa gaatatgaaa ttccttttaa gtatatagag atagaggtaa
     1801 ctgaaagtgc ttttaatgaa atatctcaac cagttatttc gattttagag gagcttaaaa
     1861 agttaggatt tttaatttcg atggatgatt ttggatcagg atactcttca ttaagtcttt
     1921 tatgttctct atctattaat ggattgaagt tagataaaag cttacttaaa gaaacattta
     1981 accgggagaa agtatatagt attattcagt gtattattga aatgtcacat cgtataggca
     2041 tgtctgttgt atgtgaaggt attgaaacta aaaaagactt ggaattttta aatacagtta
     2101 aatgtgatgt aggacaaggg ttttattttt ctaaaccgat agaagaaaaa gaatttttta
     2161 acaaatatgt aactaaaaaa taatataatt atttttatca gtctatacat tgagtcattt
     2221 tatatctcgt taaatgactc aatgtactgt tatgtatttt aaaatataaa ttatttaaaa
     2281 cgttttttat acttatatag aaattcttct atattttttg attgttctct tatttcaact
     2341 gcttctatat ctagtaaata aagtatagaa tcatttgtaa gttcaacata ttcaccatct
     2401 tctgaaccga gtgcatgtct ctcaattttt aatccttttt cttcataata tttttttgtt
     2461 tctatatata atgattctcg cttttgtatg tagtctttca cttcattatt tggaaagtta
     2521 tatgaatcca ctaatgtttc gccatttata aaagttttct cggtagcaac tttttcatcc
     2581 caacaatgga tttcttccca agtaaaaaat atctcagttc catctggaat gaatgaaaac
     2641 ctggtaataa tatataaata attattgaaa tttacttcat aatattcagg aatatcttca
     2701 aattccatat agtaatttgt ataattaaaa ttactattat cttttttt
//
LOCUS       DAEORZ010000271         2744 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEORZ010000271 DAEORZ010000000
VERSION     DAEORZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.277"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..647
                     /locus_tag="KRH39_003789"
     CDS             <1..647
                     /locus_tag="KRH39_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG8513653.1"
                     /translation="YIARIITAGFCGALIGYERKNRLKEAGIRTHFIVALASALIIIV
                     SKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLTTAAGIWATAG
                     TGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISNSTDSIEYIEG
                     KFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPLIETLEI"
     regulatory      1002..1098
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1152..1328
                     /locus_tag="KRH39_003790"
     CDS             1152..1328
                     /locus_tag="KRH39_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513654.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(1516..1755)
                     /locus_tag="KRH39_003791"
     CDS             complement(1516..1755)
                     /locus_tag="KRH39_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513655.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1900..2709)
                     /locus_tag="KRH39_003792"
     CDS             complement(1900..2709)
                     /locus_tag="KRH39_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8513656.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT          931 a          410 c          324 g         1079 t
ORIGIN      
        1 aatatatagc aagaatcatt actgctggtt tctgtggggc tttaattgga tatgagagaa
       61 agaatagact aaaagaagct ggaatacgca ctcattttat agtggcatta gcttcagcac
      121 ttataatcat tgtttccaag tatggattta atgatatagt gggtactcca ggtattggtc
      181 tagacccagc ccgcgttgca gcacaagttg tcagtggtat aggtttttta ggtgctggat
      241 taatatttat tagaaatcaa tcagtaagtg gtcttactac agctgctggt atatgggcaa
      301 cagccggtac aggtcttgcc attggctctg gattatactt agttggtatt gtttcaagta
      361 tcattattgt tattgtacag ataacacttc atagagatag gaaatggatg aaactcccta
      421 cagcagagca agtttctctt aaaatttcta acagcactga ttccatagaa tatattgaag
      481 gtaaatttaa aaaatctaat attgaaatta taaacatgaa tattaaacgc cttgatgggg
      541 attggttaga agtagttata tatactaagt tacctaaagg atataaaaaa acaaatttat
      601 taaaattatt tagcgataat cctcttatag aaactttaga aatataatat aaaaattgaa
      661 gcttaaaaat aataatatat ttaatcaagg tcaatatata aataattacg ggatgagtct
      721 atataaaatt catcccttgt tttttgccat tcatatatat tttttcaaga tatttttgtt
      781 catctattat ctagccaagt aaccctcgtg aactaaactt tttaatataa aaacttcaat
      841 acaaaactaa atataatttt tattaaacat tctatacata tagtttgttt cttgagtgaa
      901 aaatagattt catattgtta agatattgtt aaatatttca attttctgac aagtatgttg
      961 aacaataact tatcctagtg tattatttta gttgtagaat aaaactaaat cggcaaaact
     1021 agagaaattt agtgacgcaa agctataggg actaacgctt ataaaataaa ctatgagtta
     1081 tgtcagccag ttgccaaaaa gatattgttc tttttgtttt atgaaagttt tttaggggga
     1141 tagtaatata tatgtttaga gatgaaatgg ataaatgtac acacatgtta actgcttata
     1201 ttagtagttt atatgattat tgtgatttta tagatacaca gctagatgat tttatactag
     1261 agtacggaga aaatgtagta gaatcttgtt tacatcaagt gatggtattg gtaagtaagt
     1321 ataattaaat agaaattgtt ttctagagaa ccctttttag ttttatctac atttaaaaat
     1381 gttattatat taaaaaacta atctccccta tatgcaaagt tttcttccat ataacaattt
     1441 cttcccattt atactttttc acttgtatct ttaataaaat tcaacttcaa atattaatat
     1501 acttcaaaaa ttattctatt tcttatcttt acaccaattc ctatcagtat tatcctttat
     1561 ataggttcta actatttcgc cacaatctaa acataaatct gaatatatag tagattgtac
     1621 tggaaaagtt ttatttataa ctttatctaa ttttccatct cctctatatt ttaatccaat
     1681 agtttcagca tatatgccac cagttgttaa aattcccttt tcaatattta cacttccaca
     1741 attagaacat ttcacattta taacccccta aacaattatt ttttcatgct gtcttataat
     1801 ttaaaatata atttgctacc gattttataa cacagtggta aatcggtggc aaatcaacat
     1861 tttttcattc tatttaccat ctaatccatt gaaatatagc tatttagtta taggaacttg
     1921 aatttccgtt acaaactcct ctacaattcc agtctcatgt atatctatct tgtagatttc
     1981 tatagggtca gcaattatct tataattgtt ttcttctata aatttaaaca ccttctccat
     2041 atacatctta ttattagaat tactcccagt ataatttaaa gttacataat atccttcatc
     2101 aaaaactata ttatatactt tctcattact ttccagaaaa caaaatacag atttaaactc
     2161 attaaatata cctttattaa ttgcttcagt agaaaaggca gaaccaatat tgttgtttcc
     2221 aagtatatta aatctatctt catattcttt ctgaagtttt tgaattaaaa aatcgaagtc
     2281 ttcatccctc tttatatcag catttaattc taaagctttt cttttttcta tatatacaac
     2341 ctctatctca tctatttttg tattttgaat tacactttca attgaactca atctttttat
     2401 aatattttcc ttatggctta taaattcagc tattttttta tcaattaaat cgagttcttc
     2461 atttaaaata tttttcgtag actctatact tctgtcatct aggtattctt ttattttctt
     2521 cattggaaag ttcaagcttc taaattcttt tattaaattt aatttccata tatctgatat
     2581 attatacatt ctatatccat ttatatctct aactggtctt agaatgccta tttcttcata
     2641 atacattaat gaatctctac ctattccata tattttagat atctctccta ttttatagta
     2701 atctttcatt tatataataa actcctccga aatcttattt ttat
//
LOCUS       DAEORZ010000272         2639 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEORZ010000272 DAEORZ010000000
VERSION     DAEORZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.278"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            47..427
                     /locus_tag="KRH39_003793"
     CDS             47..427
                     /locus_tag="KRH39_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG8513657.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMRQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            552..752
                     /locus_tag="KRH39_003794"
     CDS             552..752
                     /locus_tag="KRH39_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513658.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            948..1676
                     /locus_tag="KRH39_003795"
     CDS             948..1676
                     /locus_tag="KRH39_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8513659.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            1698..2615
                     /locus_tag="KRH39_003796"
     CDS             1698..2615
                     /locus_tag="KRH39_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBG8513660.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTETGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
BASE COUNT         1051 a          273 c          511 g          804 t
ORIGIN      
        1 ataaaaaata ttaggtattt atttaataat aaaggtgggt attgttatga taaataaaat
       61 aggaaaaata actctctatg taaataatca agaggatgca aagttgtttt ggacagaaaa
      121 attgaacttt gtagtaactt atgaaaagga aatgggtcca aatatgaaat gggtggaagt
      181 agcaccaagt aaaaatgaat ttacaacatt tgtgttgtat gataaaaatc taatgatgag
      241 acaaaacaaa gagataaatg tatctcatcc atcaataatt ttaagtacaa gagatattaa
      301 tggaacttat gaacaaatgg caaataacga ggttgaggtt ggggaattaa tggagatgcc
      361 atatggaagg atgttttctt ttaaagacca agatggaaat gattatttgg tcagagagga
      421 taaataattt tacaaagtat taaatttact caatatctga tataattaga gacaataatt
      481 attataaact agtctaggat tagaattata taattaaaaa ttaagtgtaa attttgagta
      541 gaggtgataa aatgtttaag aaaatggcag tactaaaaga tatagcaact aaaataggtc
      601 gtaaaaaagc gtatgaacta ttagaaatgg ttgaaggtaa tgatgccttt gtagctgagg
      661 tgaagataaa aaagaatgga atagaatcta aaaaagaaga aattatgtta aaagataatc
      721 aaaaaataat attagagtat atagaaggct aatttttaaa tacatataag ttgatatact
      781 aaatttctga gatgagttta cataaaactc atctttttta cttttcaata actttgtttt
      841 attatttaat atacatttta tctgcttaga gttgctgata tataaaaatt actgatataa
      901 taaaagaatg gatattaatg taatatttta tagaaaagag gggtattatg cttgaaataa
      961 aaaatctttc ctttaatgtt gagtctaaca atgaggaact aggaataata aatgatgtta
     1021 gcctttcatt tgagagggga aaacttattg ttatcacagg gccaaatggt ggaggaaaat
     1081 ctacaattgc taagcttatt atgggaattg aaaaagctac gagtggtcag ataattttag
     1141 atggagaaga tataacaaat cttagtatta cagaaagagc aaaaaaagga ataggatatg
     1201 catttcaaca accaccaaga ataaaaggta tgacagttga aaatttactt acacttgctc
     1261 atggaaaacc attgtctact gatgtatgtt gccaatatct tacagatgtt ggattgtgtt
     1321 caaaagacta tttaaatcgt gaagttgata actctctttc tggaggggaa atgaaaagaa
     1381 ttgagatagc aactcttttt gctagagatt taaaggtttc tatatttgat gaacctgaag
     1441 cagggataga cctttggagc tttgggaaac tcaatgaaag ttttaagaaa atacatgaag
     1501 agtctaacca gactataatc ataatttctc accaagaaag aatattagag ttagctgatg
     1561 aaataattgt tctacaagat ggttcagtaa aaagccatgg aacaaaagag gctatattac
     1621 ctgaaatcat gtgtcaagtt aattcaagtt gtgaattaat gaaagacatg aattaattag
     1681 aaaggaaggc aaattaaatg aacaaaatag atttaaatct tttagaaaaa gttgctgaac
     1741 ttcgtggaac tccattagga gcatacaata tacgtaaaaa tgggcaagga gtagagagac
     1801 attcaactga aaatgtaact ataattccta aaactgataa acctggaata gatattatag
     1861 taaagccaaa tacaaaagga gaaaatgtac atataccagt aatagtaaca gagactggtg
     1921 tacaggatat ggtatataat gattttgaaa ttggagaaaa tgcagatgtt gttataattg
     1981 ctgggtgtgg tatacataac tgtggttgtg atgatagtga acataatggt atacatagat
     2041 ttttcttacg taaaggagct cgcctaaaat atgtagaaaa acattatggt gaaggtgatg
     2101 gagaaggaaa gagaatttta aatccagtta ctattgtcaa tatggatgag gattcatatt
     2161 gtgaaatgga aacaattcaa attaagggtg tagattcaac taaaagagaa atggatgcaa
     2221 acctaggtaa gggagctaaa atagttgtat ttgagaaatt attaactcat ggtaatcaag
     2281 ttgcagaatc taatatgaag gttatcttag agggggaaaa ctcttctgct caagtagttt
     2341 ctcgtacagt tgggcaagat aattctaagc aaaagtttaa tccttgtgtt attggggatt
     2401 ccctttgttc tgcacatgtt caatgtgatt caataattat gggaaattct caaattagtg
     2461 ctatacctga aattgctgca aatcatccag atgctctttt agtacatgaa gcagctattg
     2521 gaaagattgc aggagaccaa atactaaaga tgatgacatt aggtcttact gaagaagaag
     2581 ccgaacaaca aatacttaat tgcttcttaa agtaaattaa agattttatt taatttaat
//
LOCUS       DAEORZ010000273         2587 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEORZ010000273 DAEORZ010000000
VERSION     DAEORZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.279"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            137..1030
                     /locus_tag="KRH39_003797"
     CDS             137..1030
                     /locus_tag="KRH39_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG8513661.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDVL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFINEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            1472..1966
                     /locus_tag="KRH39_003798"
     CDS             1472..1966
                     /locus_tag="KRH39_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG8513662.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNAYKNFKLASKKTVINSL
                     ETYVYNMNISSISLVDTIECISLEDEQLTGKKLILVGDINTDVILDYAGNKRNRNSSK
                     KNTLKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNNLFISVTAIISYENS
                     SKIE"
     gene            1994..>2587
                     /locus_tag="KRH39_003799"
     CDS             1994..>2587
                     /locus_tag="KRH39_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513663.1"
                     /translation="MRITKTTFNDVFSNDANGSILKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKLLDVKPMKNLINKGSNLKL"
BASE COUNT         1121 a          240 c          371 g          855 t
ORIGIN      
        1 atattataac aatagtagtg cttttaatta aatatatttc ataaatcttc ataaaatctt
       61 caaaatttcg taaagaatat gtcacaaaag taaggtatat tatattcagc agatagtata
      121 agggaggaca atatttatga agaataaaaa attcattgtt ataatactga taatatcaat
      181 tttaataggt gtattagtga aggaatattc atctagagag ataaaaaagg atgatataaa
      241 tgtcagtaag tatataaaat atgctgattt agcaagtaaa aataatgcac aagttaactg
      301 gaagtatgta gcttccattg ttgctgtgtt aaataaaaat aacttaaaaa acgttaagga
      361 ttcacagata caagaagtat cagatttatt tgttaagaat ttttctaaaa ataataaaat
      421 aaataagtta agtgatgttt tggatgagtt agaatttagt aatagacaaa aaagattggt
      481 tgataattat atagacaatc ttaaggatta tggtataaaa ccagaaagat tgaagtctga
      541 tacaaaatat atgaagttta ttaacgaaat taaaacagaa gcaatacaaa attataagga
      601 ttataaaata cttccatcta taactattgc acaagctata atagaatcta gctggggcaa
      661 gtcaactttg gcaaaacaat ataacaatct atttggtata aaggctgacg cttattggaa
      721 aggtaagtcg gtgactttag aaacgaaaga acatttagat acaattatag atgataaatt
      781 tagaatatat gatgataaaa atgaatctat aaaagaccat gcaaaatttt tagccacaaa
      841 taaaaggtat aaaaacaatg gtgtatttga tgcaaaaacg tatatatatc aagctaaagc
      901 attagaaaag gcaggttata gtactgcgaa ggatgaaaat ggcaatagta tatatgcagc
      961 tcgtctaata gaattaattc aacagtataa tcttcaatta atagacagtg aaattcaaag
     1021 tgaaatgtaa ctttgattaa atttttattg ataaggctat gatttttaaa atcatagcct
     1081 tattttgaca tgttttcact tgtaattatt aaatcgatat cattatcttt caaaatttac
     1141 tttacagtgt aaatcaaatt atctaagtta aaacttggag ttatttataa aaggattatt
     1201 tattttatct aaattttgca cttaaagaac ccctttttcc ttaagactga ccgttagtct
     1261 ataaaaatta taattactta taaaaaagtc aatagatata tacaaaaaag ttctcagaaa
     1321 gacttttact tgtaaattga aattaatgaa gtaaaaaagg attctttttt aaaagtaaat
     1381 aacaactaga tagtgaataa taaaaaatgg taatttgaaa taatttttat gaatagtatt
     1441 aatatataga tacagggaga ttgatgcgat tatgtatgaa aataataagg taatatataa
     1501 ccttgaaaga tgtgaaaaac tagaaaagac tctttattta tctcaattta atgcatataa
     1561 aaattttaaa ttagcttcta aaaaaactgt aataaattca ttagagacat atgtttataa
     1621 tatgaatatt agcagtataa gtcttgttga tactatagaa tgtatatcat tagaagatga
     1681 acaactgaca ggaaagaagt taattttagt tggtgatata aatactgatg taatattaga
     1741 ttatgcaggc aataaaagaa atagaaattc tagtaaaaaa aatacactta aattaaaaat
     1801 accctttagt acatttatac aaatgcctag aaagatagaa aataaagata aaattaattt
     1861 aaagtatctt atacaagata ttacctcatc tattttagat gataataatt tatttattag
     1921 tgttactgca attataagtt atgaaaatag ttccaagata gagtaatctt taaattatag
     1981 aaaggtggat gcaatgagaa taacgaaaac tacttttaat gatgtatttt caaatgatgc
     2041 aaatggaagt attttaaaag tagaaaaata tgttgataaa aaatgtgcat ctgtatttga
     2101 gattttgacc tacactatta ttgttacaaa tacaagtagg tacaaaactg gaaatatatt
     2161 tttcaaagat tatatttcta aatatataga gtttataaat aatactgtaa aagttaatgg
     2221 tattataaaa cgtggattag acccacagca aggtttttac atagggagaa tagatgcaag
     2281 ttgtaaaaaa ataatatctt ttaagagtgt agttttacca aattctgcac ataggattat
     2341 aaagaatagt gctaatatat actattacta taagtgtaat ctagataaat tcccaacaag
     2401 aatatcaata gaaagtaata gagtttatac taatgtaaat aaaattgtat tcaaacaatt
     2461 gaatataagt aatactttaa aagctcctaa aaatctaaga gatatattaa aagtaaatgt
     2521 aaatagtaaa ctacttgatg ttaaacctat gaaaaatctt ataaacaaag gtagtaattt
     2581 gaaatta
//
LOCUS       DAEORZ010000274         2537 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEORZ010000274 DAEORZ010000000
VERSION     DAEORZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.280"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(215..1465)
                     /locus_tag="KRH39_003800"
     CDS             complement(215..1465)
                     /locus_tag="KRH39_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBG8513664.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1497..2438)
                     /locus_tag="KRH39_003801"
     CDS             complement(1497..2438)
                     /locus_tag="KRH39_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG8513665.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLELVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          755 a          490 c          238 g         1054 t
ORIGIN      
        1 taaaacctta taaatataca aatacaatta tataaatttt cattctcaat tataacatag
       61 aatatggcta tctatatccc aaaattatcc atttttcaaa aaaggtaact attattaaga
      121 atatcaactt atataatttg gattccttat acgattttca attaaaaaaa caagggctaa
      181 aaagtgaatt gtacactttt tagcccttta ttaattattc atttatttca tgtaagaata
      241 gcccacatct tactttaggt tcaaaccatg ttgacttagc tggcattatc ttatttgcat
      301 ctgctactct tattaaatct tcaatactag taggatacat tgcaaatgct actttcatat
      361 cttcattaac tcttttttct aattcttcaa gacctctaat tccaccgata aaatcaattc
      421 ttttatctac tctaggattg tcaattccta aaatagcatc aataacatag tcttgtaata
      481 tagaaatatc taatgagtca acagggtctt ctatttctaa taaattttta tttgctttca
      541 ttttatacca cttatcacct aggtacattc cgaaagttcc ttttttgtca ggtttatatt
      601 tttcttttcc ttctatttct tctagttcaa aattttcttt tattttattt ataaattctt
      661 cctcgcttaa accatttaag tctttgataa ccctattata atccaaaacc attaaattct
      721 catctggtgc aatcattgct atgaagtaat taaattcttc ttttccagta taatttggat
      781 ttttagctct catttctttg gctacatttt ctgctgaagc tgtcctatga tgaccatctg
      841 caatgtatag ataatctaat ttttcaaatt tatctactag gttatctatt atatcatcat
      901 tatcaatctt ccatactgta tgctctatct tgtcatctgt tatgaaatca tataaaggtt
      961 cattattatc cataaaatca ttgattatat catctatcac cctttgatgt ttgtaagtta
     1021 caagtatagt tcccgtatta gcatcacagt attttatgtg tttagttcta tcaatttcct
     1081 tttctggtcg agtgtactca tgttttttta ttgttccatt caagctttca tcaactgata
     1141 tacatgctac tataccaact tgagctctac catccatgat ttgtttatat atatatatac
     1201 actctttgtc atcttttact agaatttctt gttttcgaaa ctcatctaaa ttttctcgcg
     1261 cttttaaata aactttttca tcatataaat ctatattttc atctaaatca atttctgatt
     1321 tatctatatg caaaaatgaa tatggattcc cttctgcaat tcttctagct tctttactat
     1381 tcataacatc gtaaggaagt gctgctactt tatctactat atatttattt ggtctaatcg
     1441 ccttaaatgg tttaatcgtt gccattacta atttcctcct aattatacaa aatattttat
     1501 aatgctaccc ctattagatt gttgaagtca agaaagaaat cttgaatcac atttactatt
     1561 tcgtctccta ttctttcttg agcttctact gtagatgccc caatatgagg tgtaggagaa
     1621 actcttggat gatttactaa ttctaaattt aatgtaggtt cttgttcata aacatctata
     1681 gcagctgctg ataatttacc ttcatctaat gcttttagta aatctttttc atcaactaaa
     1741 ccgcctcttg cacagtttat taaataagcc cctttcttca tcttatttat ttctttttca
     1801 gttattactg cccccttatt cttatcaaat ggtatgtgta aagataagaa atctgcattt
     1861 tctaaaactt cttccatatc acagaattta taattgttaa agccttgcgc ctcacccatt
     1921 atgtcagtat atataacatt catacctaat aattctgctc tctttgcaac ttcttttgca
     1981 attcttccaa aacctataag tccaagtgtc tttccattaa tttctgtacc tttatacttt
     2041 ttcttctccc attttccatc tctcatagtt acatttgcag tatttatgaa tcttgcaaga
     2101 acaaataatt gacctattgt aagctctgca acagatatac tgcttgcatt tggagtattc
     2161 ataactttta tgccattagc ttgagcatat ttaacatcta tattatccag tcctactcca
     2221 cctcttacta tcaacttcaa ctttttaccc tctaatgctt tatctattac atctttagta
     2281 actttagtag ctgaacgtac tacaagtaca tcaacatctt taagtttatc tcctaaaaca
     2341 tctttttcat aaaactgctc tatcacatta aaatctaact ctctcaattt tctcgctgct
     2401 tctttctcta ttccatcagt aactaatata ttatacataa caaactcctc caatttttat
     2461 tcacttaatt tttccaattt ttatttttta aagtattttc atcaataaaa aactttactt
     2521 aatttaactt tctgttt
//
LOCUS       DAEORZ010000275         2449 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEORZ010000275 DAEORZ010000000
VERSION     DAEORZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.281"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(223..1071)
                     /locus_tag="KRH39_003802"
     CDS             complement(223..1071)
                     /locus_tag="KRH39_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG8513666.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            complement(1107..1577)
                     /gene="trmL"
                     /locus_tag="KRH39_003803"
     CDS             complement(1107..1577)
                     /gene="trmL"
                     /locus_tag="KRH39_003803"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBG8513667.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            complement(1771..2427)
                     /gene="nth"
                     /locus_tag="KRH39_003804"
     CDS             complement(1771..2427)
                     /gene="nth"
                     /locus_tag="KRH39_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBG8513668.1"
                     /translation="MKNESANEKDVNKILDELEKLYPDAKCELNYGTAFELLIATILS
                     AQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLC
                     ELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPN
                     PQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK
                     "
BASE COUNT          759 a          462 c          255 g          973 t
ORIGIN      
        1 atttttttat atacaattta caagtacatt ttgaaatttt tttaataaat cttactataa
       61 aaaagtgtgc aatagattct tatctattta cacacttccc tacaaactaa atctaatata
      121 tcaacactta atttcatcca aaataaatcc aatttaacat ctcaataatt aatattctat
      181 cctaatttaa taaaaaaata aataatgtca tccaaaaata tattatttat tttttaagca
      241 tatgatacca gtaacaccag gaccagcatg tgcacctata cacacaccaa tttggaaaaa
      301 tctaatttct ttaggtctta attcatcttg cataacctta gataattgtt ccttttcctt
      361 aaaatcatca ctatatccta tatacacaat ttggtctgat aaatcatcac cacaatttga
      421 ttttatcaat tccatcatct tagaaattac attcttcttt cctcttactt gagatatttg
      481 cgaaactaat ccatctctta tttcaagaat cggttttata tttaatatac ttcctattgc
      541 tgccttagta gatgatattc tacctccctt ttgtaaatat tccaatgtat ctactgaaaa
      601 tataacaaaa gccttctctt ttaattcatc caaaacaggc aatatttcag caacagactt
      661 tccttcactt gcaagttctc ctgcttttat tacaagtaaa ccagttccca tacaaagcaa
      721 gttagagtca tatgtatata ttttaccatc tacatcattc tttgccatta cagcactttg
      781 atacgtacct gtagctgtgg cagaccctgc tatataaagt atctctctat cctcatttgt
      841 atatttctca aaaatttcct taaattgggc ataagtagct tgagaagttt ttggatatac
      901 attttcttct ctaagccttt tataaaattc atctccagat atatcaattc tatccttgta
      961 ttctctttcc tccagtataa ctgttaatga aactatatct atatcatatt tatctaacaa
     1021 atcttttggc acgtccgata aactgtcaca gattatcttt agatttttca ttaagtatgc
     1081 tcctttctca tttaggcttg ttgaaactac ctcatatttg ggtatcctat ttgtcttaaa
     1141 gcctcgtaca ctacaatagc aactgaattt gataaattta atgacctagc tttttcaaca
     1201 tccaacatag gcactctaat acaagtatcc atatttgctt tcaacaaagg ttctggtaat
     1261 ccctttgttt ctttcccaaa tactataaaa caattttctt catatttcac atctgaatgt
     1321 gactgtttag cctttgttgt agaaaaaaag tatttactat ttggatactt ttcttgtaat
     1381 tcttcaaagc tatcgtaata ttgtatatca gctatatccc aatagtcaag cccacatctt
     1441 tttaggtgtt tatcatccaa tgaaaaaccc agtggtttta ctaaatgtaa agttgaacct
     1501 gttgctgcac aggttcttat tatatttcct gtattttgag gtatttctgg ttctactaat
     1561 actatattta atgacatttt aatcctccct aagtatcgct tttatttatc aaattttatt
     1621 ttataaacag aataacaata atattatact attatataaa ataatgttaa taataatttt
     1681 tatttcatgt atataatagt tttttatcta acattttcac aagtattatt atatcagaaa
     1741 atccctcttg tacaacttac tttctaaaag ttatttcgtt tcattaagtt ctttataata
     1801 attacaatct tcttttattg ggcaacttgc acattctgga tttctagctt tacacattct
     1861 tcttccatga aaaattaata catgatgtga gtgtgaccat ctttcttttg gtatagcttc
     1921 cataagtgca aattctgttt tttgaggatt tggctcgtcc actattccta ttctattgct
     1981 aactctaaat acatgagtat ctacagctat tgctggatga ttaaaagcgt tactaagtac
     2041 aacccctgcc gtttttcttc caacaccagg taattttata agcttttcta agctatcagg
     2101 tacttcacca tcatataatt cacatagctg ttcagatgta tcctttattt tttgagactt
     2161 gcttttgtaa agaccacaac tctttatctc tttactaatt tcttcaatgc tcaaattagc
     2221 aaagtctcta gctgtgttgt actttttaaa caactctgaa gtaactttat tcactcttac
     2281 atcagtacat tgtgctgata aaatagttgc tattaaaagt tcaaaggctg tcccataatt
     2341 taactcacat ttagcatctg ggtacagttt ttctaactcg tctaaaatct tatttacgtc
     2401 tttttcattt gctgactcat ttttaatttt agacttactt ttagtttct
//
LOCUS       DAEORZ010000276         2364 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEORZ010000276 DAEORZ010000000
VERSION     DAEORZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.282"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            53..526
                     /locus_tag="KRH39_003805"
     CDS             53..526
                     /locus_tag="KRH39_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8513669.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(895..1452)
                     /locus_tag="KRH39_003806"
     CDS             complement(895..1452)
                     /locus_tag="KRH39_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8513670.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            1710..>2364
                     /locus_tag="KRH39_003807"
     CDS             1710..>2364
                     /locus_tag="KRH39_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8513671.1"
                     /translation="MEKKYFEIHGVPSIIWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     "
BASE COUNT          866 a          317 c          296 g          885 t
ORIGIN      
        1 ttatttaaga tatataatta taattaaaat aataataaag gtgttgttta taatgttcaa
       61 agaatactta ataagagaat taaaagaaga tgatattaaa aatgcattag acttaatctg
      121 gaacgtattt gaagaatttg aatctccaga ttattgtgat gaaggaatta cgacatttca
      181 tcaatttata gaatataact ctattattga aaaaatcaaa gaaaaaactc tcttcttttg
      241 gggttgtttt gactctaaaa aacttattgg agtcttagct attagaaatt taaatcatat
      301 atgtatgtta tttgtagata aaaactatca taaacatggt gttgcaaaaa aattaatgac
      361 tacaggcata caatactgta tttgtaataa atttaattta actgaaataa ctgtaaattc
      421 atcaccttat gctgttgaag tttatcataa attaggattt aaagatactg ctgttgagca
      481 aattgttgac ggtatacgtt ttacacctat gaaattgtta atataataaa ttaaataaaa
      541 agcaaagtct cttaaatatt ttatagttac tttgctttta aaccaagatt taaaataata
      601 ctatcttttt tgttttttaa taatatactt taaaatcctt actaatatga actataattt
      661 attttttgat atcctttttg attaacatat aaattaattc aattattaaa cataaactat
      721 gttgtttctc tttatatctc ttctttgtaa acaatattgg gcatttgaaa ataaatattc
      781 ttaaaatata ctgttctcct gttgattttc ctttggtaca aatgataaaa ttttaattta
      841 catgctccgt aatgaagtgt tcaataaata agttaaaata atttatttac ctccttaatc
      901 atatttttta tgaataccta ataacacagt atcaatagaa tcgactaaac cttttatagc
      961 ttcttcttta ctcaaaagtg atttcccaag ttgcaaagtc gaataaccat gaagtacact
     1021 cattattaag tttaatactt cacctgtatt ctttgtattt agattacaag aaagaataag
     1081 tttttctaat aaggatttat attcatcaaa gatttctaat gtttcattat ttccatgcca
     1141 agtagcccac tgaattgttt cataaacacc aggattagaa attataaaat taaagtatgc
     1201 tatacttata cactttatag ctgtatctcc aaaatttcca atagccgcct ttatcatttg
     1261 actattcatc tctctcattc ctttatgagc tacttcaagt aataaatcat ctagactttc
     1321 tatatgattg tacaaagatg gtgtacgtat tcctagcttt tctgcaataa cttttaaaga
     1381 aactttgtta agcccatctt tattaactat atctgatgta gcttgtataa ctatttctct
     1441 actaacacgc atgattttcc ctctttcatt taaaattaat tactttatta gtttagctaa
     1501 tctaattagt ttcgtcaatt attgtaattt ataaaattct ttatactatt ataaaatctt
     1561 gacagtttaa ttataattta atataatact aattgaatta gttttaatac taatataatt
     1621 agtattatat taaattctat attacaacat tatcttatag tttgattaat ttaacaatca
     1681 ctaatatatc aaactgaaag gagattttta tggaaaaaaa atatttcgaa attcatggag
     1741 ttccttcaat catttgggga aatccttcaa gtaaattata tatttacata catggacaat
     1801 gtggcaataa agaggaggct gaagcttttg ctaatattgc agttccacat gagtgtcaag
     1861 ttttaagtgt tgatttgcct gaacatggag aacgtaaaag agaaaaaaat tcatttaacc
     1921 catggcatgt tgttccagaa ttagtatcta ttatggaata tgcaaagtgc cactggaatc
     1981 aaatttcgtt atatgcaaat agcattggag catggtttag tatgttaagc tttgaaaaag
     2041 agaacttaag agagtgtatt tttgtatctc caattctaga tatgcaacag ctcatttcaa
     2101 atatgatgct ttgggcaaat gtatcagaag aacagcttaa acatgaacta attatcccaa
     2161 cttcatttgg gcatactctt tcatgggaat atctacaata tgtaaaacaa catcctatca
     2221 ctcaatggaa tgtaccaaca aaaatattat atggtaaaca tgatgaactt actgaaatcg
     2281 gtgttgtaga gaaatttgta gaaaagttta attgcaacct tactattata gaaaatggca
     2341 aacattggtt taacacacca caag
//
LOCUS       DAEORZ010000277         2333 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEORZ010000277 DAEORZ010000000
VERSION     DAEORZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.283"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            109..426
                     /gene="trxA"
                     /locus_tag="KRH39_003808"
     CDS             109..426
                     /gene="trxA"
                     /locus_tag="KRH39_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG8513672.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTILIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            complement(632..1906)
                     /locus_tag="KRH39_003809"
     CDS             complement(632..1906)
                     /locus_tag="KRH39_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG8513673.1"
                     /translation="MQFMDVSKYIEGANLKVKTTNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDSKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYEKELFLYAKEEMSKIPNVIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYIRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIYAIKTLAFK"
BASE COUNT          829 a          306 c          335 g          863 t
ORIGIN      
        1 taatattaaa tctattaaaa ataggcggtt acaaaaaagt gcctaattga actgttatat
       61 aggtcgtagt atattaatat caagaagaca ataaaggaga tgtatattat ggtaaaaata
      121 ataaataata atgaatttat aaatgaagta gaaaataaag atggtttagt agttgtagat
      181 ttctttgcga cttggtgtgg accttgtaag atgctatcac caatatatga agctttagga
      241 aatgagatgg ttgaaaaagc aaatttcttg aaggtggata tagaccaaag tatagaatta
      301 gctcagaaat ttgaagtatc tacagtacca actatactaa tatttaagga tggaaaacct
      361 gtagatagat taataggttt tatgcctaag gagaatttaa aaaataaaat tgaatcatat
      421 atgtaattaa attaaattgc agtaattaaa ataaatctca tatataaaca agaaactcat
      481 tgtaatgagt tttttgttta tacagtgtaa aatagtttgt atagttacta atgagtgcct
      541 aattgctaat gagattgact gttgatataa tttgcctttt tgtgaaaaca aaactattct
      601 ctatttattg ctaaaaaaga atagaaaaat cttatttaaa agccaaagtt ttaatagcat
      661 atataaagct atcaacctca tcaaaagtat tgtacattcc taagcttatc ctaacaagac
      721 ctacaggatt ttccctcata aatctgatgt atgcctcttt tggacttata cctaacaatt
      781 tttgtacata aatatctgca cctactactc cagctccagt ttctattcca aagttatcaa
      841 ctaaatattt tgctacatct ttgtaataca tattacttat attgaaagat atatttggga
      901 taattacatt atttgattta tctccatata ttattacatt aggtatttta ctcatttctt
      961 ctttagcata taaaaataat tctttttcgt attttgaaat tctgtttaga ccaattttat
     1021 ttaaagtgtt tagtgctttt cccattgcaa gagttccaaa atactctggg tatcctgctt
     1081 caaaacgttg gggcacgttt gcatatagta ttttgctatc atttacaaat ttggtagaac
     1141 ctgagccata tagtacagga ttaaattttt caaagaattt tttaggtcct accagagctc
     1201 ctccatttag tggagaataa catttatgag cactaaaaac aacaaagtct atatgctcat
     1261 cacattcatg tggcatcatt gaaaaaggtt tatgctgaat tacttgcaca atatccacca
     1321 atatctttgc accatactta tgagctattc ttgctatttc atatatagga gtggttatac
     1381 ctgtgacatt actagctcct gtaactgtaa cgagagatac attaccagca taatttttaa
     1441 gtatattctc taattgacat aaatcaagtt ctcctttttc tgtaattcca acaacttttg
     1501 taggaaaatt aactttaaaa ggcaggtaat tagccatatg ctccattgct gtggtaataa
     1561 caaatttatc tggattttca tctttaaaaa attttgctaa gaagtttatt ccttctgtag
     1621 catttttgac ataaataaca gaatcctttt cttcgtctcc atcaataagt ttgaggatag
     1681 tagagcgtac attttcatat tttaaagtat ttttttctga gattattcct ggagcattaa
     1741 tataagtaag ccaaggaatc tcacagttta agttatccac tacgtttttt aaaactaatg
     1801 gagtggcaga gttgttaaaa tatattcttt taacagtttc accatttgta gttttaactt
     1861 taaggtttgc accttcaatg tatttagata catccatgaa ttgcataaaa aaacccctta
     1921 tctaataaac tattctgttt tttattaaaa aagctcaagt tttaatcaag agcttgttta
     1981 atatatgtta tgtttttata aggggttttg ttaattaatt cttgttgaga tgtttattaa
     2041 attacacgaa aataagctac agttggggga aactgtagct cacttgattt tgggggagat
     2101 aggttgaact tattgtttat ccgtatcata tttttagtat agtcacttat tttaatttat
     2161 atacattttt ttataaattt tacataaaat gttaaacact aattagaaat aattatgaat
     2221 atgactaata aagttgtaaa aatagtaaag aaattgatta gaacataatg gaaaatatat
     2281 tgtatataat tagaaaaaaa gattttatat gttataaaag atgtacaagt ata
//
LOCUS       DAEORZ010000278         2331 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEORZ010000278 DAEORZ010000000
VERSION     DAEORZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.284"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            152..1453
                     /locus_tag="KRH39_003810"
     CDS             152..1453
                     /locus_tag="KRH39_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8513674.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1529..1804
                     /locus_tag="KRH39_003811"
     CDS             1529..1804
                     /locus_tag="KRH39_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG8513675.1"
                     /translation="MLELRKIIELFEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVKKK"
BASE COUNT          953 a          186 c          467 g          725 t
ORIGIN      
        1 tataaaacaa tgtatcagaa taaatagtat attaataatt tgtagtatat catgacgaat
       61 aatacagtca taacaaataa tgtatttata attgataatg tatttaaaaa tcaaaatata
      121 aaaacttata aggaaagaga gattaattta tatgggtaaa tcaaaagatg ttgaaataga
      181 gaagtctatt ttaggcacaa ttcttttaga caataaatta agctataagc tagatgaatt
      241 aaatgaaaac atgtttatga atgatatatg tcttgagata tttaaaatta tgaaagaact
      301 taaaaaagaa aacatagtga ttgatgtggc cactgtgaaa tcaaagatag atagaaaatc
      361 tctagcaata aaaacgagtg atgtgactaa tctaattact tggggtcaga attttggtct
      421 tgatggtcat attaaaattt taaaagaaaa tttagctaga agaagcataa atcaaaactg
      481 ccaaaacctt ttacacagcc taaaccttgg agaaaatatc gatacttgta tttacaaatt
      541 tgaaagtaat ataaaggaga ttttggacaa ggatacttat gaaaatgatg atgtaaatag
      601 tatagcaggg aaggtgcttg attttttaga aaacaaaaaa gatattggat ttaaatttgg
      661 tataaaatta cttgatacca ctataggagg tctttttaaa ggagagctaa ccacaatagc
      721 agctaaatcg ggggttggga agactgcttt ggcacttcaa ataatgttaa attcatttaa
      781 gcaaggtaaa aaaactttat ttataagtag agaaatgacc agtgagcaag tatttatgag
      841 aaatatatgc agagtaactg gtgtaagtac aaggaatatg aaatctaaag aaatagatga
      901 aaatgattgg aaacttattg taaatgctat aggtgattta agcgaaaata acttaattta
      961 tatcaatgat aagatagata caattagtgc aattagaaag aggataaggc aggtaaagcc
     1021 agatttatta atagtggatt atgtgcaact ccttacatct cagaaaagca tggataagag
     1081 agaaagggaa gtagctactt tttcgagaga acttaaaaat atgactttag attttaatat
     1141 acctgtaatc caattaagtc agttaaatga tgaaatgaaa gattcaagac cttatggaga
     1201 tagacctatg agggatagca aagccatcta ccacgatagc aacaatgtgg tttatataca
     1261 tcaattaaag gggagtgatt atgaagaagc agtgagagat attggagaaa gtgaggaggc
     1321 agttagagca tcagaataca ggggaattaa gatggttgat ttaatagttg cgaagtgtag
     1381 agatggacag actaggcata agcatttttg ttactttggg gataagttac attttcaaga
     1441 actaaattat taggtcaata aagtagtaat aacaagctta ttaaaagtcg ggaaatagca
     1501 aatagattat aaaatatgag gtgaaaatat gttggaatta agaaaaatta tagaattatt
     1561 tgaagaacac aagatagaag taagtattcc aaaggagtta cataagtata gtttattgga
     1621 ataccttgaa ttgtgtcaac aaagtggggg aacatatttt tatattaaaa cactggaagg
     1681 ggctttaaaa gaggctagaa atgagcttat gattaaattg tttaatggga aaaattatat
     1741 ggaattagcg aggatgtttg aaatgagtgt tattaatgtc aggcatattg ttaaaaaaaa
     1801 atagatattt ttggagaata caagtgtgat atacttctga aatagaagtt attatagttg
     1861 aaataaaatt gtggttaata aattaaagtt gtgtataagt ttaagattat agaggagtat
     1921 aagtgtgatt aacagcatga ttttgtgaat aaaattgagg atatatatga gttaaatgtg
     1981 tgattaataa ggtaaggttg tgtataagat tatgagtatt taaaaggagg ggttataatt
     2041 aatagtgtag agttgtgtgt ttgcttaaga ttatataata atttaataga ttaaactata
     2101 ctaataaggt aaaatcgtgt gtttagttaa agcttattat aaaatcttga tatgtgatta
     2161 atgatgcaat atgtgaataa agttaagctt ataatagatt taacctgtgg ctaatagaat
     2221 aaaattgtga ataaagttaa gattataata ggtttaacct gtgattaata gagtaagagt
     2281 gtaaatagag ataaacttca atgtgggttt aacttgtggt taatagagta g
//
LOCUS       DAEORZ010000279         2318 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEORZ010000279 DAEORZ010000000
VERSION     DAEORZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.285"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(157..1449)
                     /gene="brnQ"
                     /locus_tag="KRH39_003812"
     CDS             complement(157..1449)
                     /gene="brnQ"
                     /locus_tag="KRH39_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG8513676.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          777 a          381 c          249 g          911 t
ORIGIN      
        1 ataaatcata ctacttcaaa aataaatttt taaatttatc aaacaggatt aataatgttc
       61 ttttatctac aataatttaa taattctttt aaatataaaa aggaattgcc tatataattt
      121 aaaggcaatt ccataattat caattagttt ttattattat gcagttttac tgcttttaga
      181 tggaataaag tttcctatta ttcctgctat tacaactgga actatccagt taaatcctaa
      241 tgaagcaaat ggtaagctat taactagagg aatttttaca ccaaaacttg ttgcaactgt
      301 taatacactt actaataatg ccatgtatgc agcacatttg aacacgttgt catttttaat
      361 tttattacta aataatgcta gtataactag agttatagtt gctggatata ccatacttag
      421 tataggagaa gagaattgta ttattgtact tactccaaag tttgatatga ttgcactaaa
      481 tacacaaact actataacta tatgctcata ctttaatttt ccatctgtaa gtgttgagaa
      541 atattgacca gctgctgaag aaagacctat agctgttgtt aaacatgcta atgcaactat
      601 aatagccaat aaaattttac ctgtagttcc aagtaaagct gctgttattg caactattaa
      661 agctgtttgt tgaacatctt gtccatattg ttgagacaca gttgctccaa gataacataa
      721 tccaccataa actaatgcca ttcctattac tgcaattaca cctgcactta ttgtggcttt
      781 catcttttct ttctcacctg tataaccttt agctattact gacatgatta taattgatgc
      841 aaatacacta gctgccaatg catccattgt ttgataacct tgagttatac cttctgcaaa
      901 tacattttgt attaaagaac tgtctcttat ttctcctaat ggagatacga tacctttaac
      961 tattaatact gccaaagcta taagtaaagc tggagttaat attgacccaa ctatatctac
     1021 aacttttgat ggtcttattg ttaaaaccaa tactagagca aagaatacta tagagaatat
     1081 tactgggctt attccagaac ctattgtagg taatataccc atttcataag ttgttgctgc
     1141 tgttctaggt attgctaaaa gtggccctat acagattacc attgcacatc ctaatgcaat
     1201 acttaatcct ttgcctgctc ttgagaacat cttcgatgtg tctccattga acttagttgc
     1261 tgctataact gctaataatg ccaaaccacc atctgcaaaa aggaatccta tgaaagctac
     1321 tagccaacta cttccagata aaactcctag atatggaggg aagattaagt tccctgcacc
     1381 aaaaaacatt gcaaatagtg caaatccaat tacaattaca tctttaaaat tgatttgttt
     1441 ctcacccatt taaagcaccc ccatttgaat tttactacct tttactaaaa aataagtcca
     1501 atctcttaat taaatgaaaa ccttttgacg agattttatt taattataca tttttgactt
     1561 aaatttgtca attatgtttc ataatttata aaatcttcac aatatttaat acaactttct
     1621 taattaaaca aaatcaacct gtatacttta aacatagttg gttttttgtt ttttctctaa
     1681 tttttcttga taaaattaac tattccgaat aattaaaata ttaagacttt ttagattatt
     1741 cgatttatta ttaagttgta ttgctttata tactgtatat atcactctcc ttttttacat
     1801 tcatataatc tttttaattc aatttaaatt ttaaatattt tgtgtttatt atataatctg
     1861 aaaaaaaagc agtcaatagc atttttaatt tttttaattt tcaaaattat gattcttaaa
     1921 aaatcgtttt ccttttatca taacacctat ttacgttaat tcttgtagta ttttgtattt
     1981 attttgctaa attgacagtt aactttattc aatttttatt ctacttgcgt agatttgcct
     2041 cttgtttttt actcttaaaa ccattttttc tgaaaattcc ttattatgat ttatcaactt
     2101 atttttcttc ataacaatta ctctctaact tccaacttac ttaaactttg cagttgctag
     2161 tattcacgtt tacatactta tatgtcataa ttaactaaaa attcttaata tatctctcaa
     2221 tgaaaacaat ttactcattt atttaataca tttatataat catatgtctt aaattttgtt
     2281 ttattatttt attattgtca caattttatt tatattat
//
LOCUS       DAEORZ010000280         2288 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEORZ010000280 DAEORZ010000000
VERSION     DAEORZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.286"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            176..1033
                     /locus_tag="KRH39_003813"
     CDS             176..1033
                     /locus_tag="KRH39_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8513677.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            1130..1999
                     /locus_tag="KRH39_003814"
     CDS             1130..1999
                     /locus_tag="KRH39_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8513678.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT          963 a          215 c          385 g          725 t
ORIGIN      
        1 atatggctat ttgctttata aaatactaaa agaaaataat atataaaaaa caaaaaacat
       61 atatttatga attttatatg aaaatagtta aaatattgat ttttttcatt ggttgaaaaa
      121 acaatttact taggataaac tataatagta ttcaaatatt atgggggtta gatttatgag
      181 taaagatgta aaggtaaagc gtaataagaa atatgataca tttagtataa ctatggaact
      241 tgagagacct ttactttatg atgaggttga gaacaaagat gataaaaata ataatagaaa
      301 atttaacata aagaataaga tatttaaggg aataggtata ctatttgtat tgattttgct
      361 tggctttttc atatggataa ataaaacata tgaacctcaa aagctggcaa aagaagctct
      421 agtcagcaat agtaaagttg aagtaactgt aaatgaaaac atttcattta caccaaaagg
      481 aagaaaagtt tctaaaggtt taattttgta tccaggagct aaagtagaaa tagaatctta
      541 tgctccactt gcaagaaaaa tagcagaaag tggatatgaa gttgtaatcg taaagatgcc
      601 acttaatcta gctatacttg gaactaataa ggcacagaaa gttatggatt cttacaacaa
      661 catagaacat tgggtaatag gaggtcattc attaggagga gttgcagcat caaattttgc
      721 acgtgataat aaattaattg aaggcgttgt atttttagct tcttatccaa tgggtgatga
      781 gttgaaagaa ttaggaaaaa aagttatatc tatatggggg agcaaagatg gagtagtcaa
      841 ttttaaaagt cttgtagaat caaaacaaaa acttcctgat gatacaactt atgttgagat
      901 tgaaggagga aatcatgcac aatttggaga ttatggaaaa caaaagggag ataatgatgc
      961 tattataagt caagaaaaac aacttaatat tactgccaat agtatcatta aatttttaaa
     1021 gaatatatct taaactaaaa tttaaaaaat ataattttat aagttattat ttaaagtaaa
     1081 tattaataat ataatagaat gaatatttta ttacaagggg gattttaata tggaatacga
     1141 tatgccactt tacagaccac caagtgaggc ttatagtctt ataatacaag tcacactagg
     1201 atgttctcat aacaaatgta cgttttgtag tatgtacaaa tctaaaaaat ttaaaataaa
     1261 gccactagaa gtaataaaaa atgaaattga tatatttaga agatattata agcatgtaga
     1321 aagaatattc ttagcagatg gagatgcatt gataatacca atggagaaat taagagatat
     1381 tattttatat ataaaagaag tttttccaga gtgtgaaaga attactatgt acggctctcc
     1441 taaatctata gagaaaaaat ctgatgatga acttaaagaa ttaagaaaac ttggtgttaa
     1501 aatgatttat ctagggcttg aaagtggaag caatgaagta ttagaagata ttaagaaagg
     1561 ttttagtagt gaacaattaa taaaagttgg tagaaaagtt aaaaaaagtg ggattaaatt
     1621 atcagccact gtaatagcag gtcttggagg aactaaaaaa acacatcagc atgcagttga
     1681 tacaggaaaa atgttaggag caatttcacc agattatgtg ggtgtactta gtttaatggt
     1741 agaacctaat actgaacttt acgatttact taaggcagga gagtttacaa tactagagga
     1801 taaggctgtg ttacaagaaa ttaaagagat gataaagaat atagatgcta atgaaaaaat
     1861 tgtatttaga agtaatcatg catcaaatta tgctaattta aaaggtgtat tacctgatga
     1921 taaacaacgt ctaattgatg aaattgatta ctatctaagt aaccaaaagc taaaaagaga
     1981 agaatataga agactatagt ttttgatata tcaatctata ttgataggta aatactaaga
     2041 ttatttagat acaaaaaatc atgctacatt aatagttgct tactattgat gtagcatgat
     2101 ttttttatta aaaaacaaaa tggtatattc atataataat atatcaataa taatgaaaat
     2161 gtgaactagt ttaatagaat tttacctatt ataataaaaa caggaattta aacaataaaa
     2221 agtaaagtaa ctttttatta agtaacttta ctttttattt tatttaatgt tgtattaaat
     2281 ttatatat
//
LOCUS       DAEORZ010000281         2283 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEORZ010000281 DAEORZ010000000
VERSION     DAEORZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.287"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            510..1913
                     /locus_tag="KRH39_003815"
     CDS             510..1913
                     /locus_tag="KRH39_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8513679.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEVCYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
BASE COUNT          897 a          318 c          325 g          743 t
ORIGIN      
        1 tatattatat tctaatattc tactcaacaa atcctatatt atattctaat attctactat
       61 actacacaac aaaccctata ttatattcta atatgctact atactacaca acaaatccta
      121 cattatattc taatatacta cccactattt tgcgctacaa caatttctct atttatatga
      181 tttgtaaaat atagtacaac attttttatc attttcattc cagctaaatt tgtaatattt
      241 tattcaatgc ataatcaata attaatcaat acatgcttta taatacctta ctcaatatat
      301 aatcaataca taatttataa tttgtaatac tttattcaat acataaatag aaatctctat
      361 atacataaat tcactttttc atacatctta caaatttatt tttatttaga taacatgtat
      421 attttttcca tgaaaaataa ataataaaaa atagtaaata tggaaaatat aaaattgatt
      481 aacaataatt aattacctga ggtgtgaata tgaaaatgct aaacaacttt tttaagaaaa
      541 tcaacaatcc agctcctgtc tttgcacaga caaaaatgga aagagataat caaacagatg
      601 attcactttc tacaaaacca aagactacat ttagagacgt tgctggatta gacgaagtaa
      661 aagaagaact atttgaaata gtcgatttta tgaagtcacc acaaaaatat caaaaaatgg
      721 gtgcaaaaat acctaaagga gtattatttt atggtcctcc aggaacaggt aaaaccctac
      781 ttgcatctgc tgtagctggt gaaacaaact catcattttt caatgttacc ggttcagagt
      841 tcgttgaaaa atatgtaggt gtgggagcaa aaagagttag aactctattt gaaaaagcta
      901 gaaaagaagc tccaagtata atatttatag acgaaataga tgctgtagga gcaaagagac
      961 atcttgaaag taacaacgaa aaagaccaaa cactaaacca acttttagtt gaaatggatg
     1021 gatttaataa agattccaat gtattaataa taggtgctac caatagactt gacttattag
     1081 atgaagcttt acttagacca ggaagatttg acagacacat tcatataggt gcaccaaact
     1141 atcacactag atttgaaatt ctaaaggtac atacagatga caaacctata gacaaatctg
     1201 taaatctaga acttttagct aaaaaaactc atggtttcaa tggggctcat ctttcaaata
     1261 tagcaaatga agcagctata tttgcagtta gagatgacag tgagtgtatt acatcagaac
     1321 attttgacaa ggcacttgaa agagttattg cagggctaga atctaaaaac tctgcattgg
     1381 ttgaaaaaga gaaaaaaata gtagcatatc atgaagctgg acatgctctt gtaagtgata
     1441 tagtaggaat ttgtcctata caaaaaatat ctatagttcc tagaggacaa gcattaggtt
     1501 atgttcttca attacctgat gaagatagat atatttatac taaggacgag ttgattggaa
     1561 aaataaaaat attacttgct ggaaaggctt cagaagagtt aatatttaat cataaatcta
     1621 ctggtgcaaa ggacgactta aagaaagtca ctgaaatagc taaccaaatg gtttgtgaat
     1681 atggtatgag taatttagga tttatgacta ttgatggaaa tgataagact ttcttatgtg
     1741 ataaggttca aaaggaagca aatcgtatag ttgaagtttg ttataaagag actttagaga
     1801 tgttaaagga taatcttgaa gatttacatt ctgtttctaa attcttattt gaaaaagaaa
     1861 ctatgactca tgaagagtta aaagatttga taggaaaaga agctgtaaac tagtatagtt
     1921 ttaaaaattt gaggctgtct caaaatagag ataagcctct ttttattgtt aaagttataa
     1981 aaatatattt gatttcatac aagaaagtgc ctcataaact aaaaaattca ttttgagaca
     2041 cttccatatt ttaattttgt ctatttgcat aatattatat ctacataaaa ctaaactgct
     2101 gtaaacttaa ctacctccag taatgtttga agtggtataa aagctagtag cctagtttct
     2161 acacctataa tttatatttt acataactaa atttagtaga tttttatact cacaatttag
     2221 aacaattgga aacttaaaaa tttcttatca gacatctaca acttaattag gtatctatca
     2281 ctt
//
LOCUS       DAEORZ010000282         2114 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEORZ010000282 DAEORZ010000000
VERSION     DAEORZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.288"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..1824
                     /locus_tag="KRH39_003816"
     CDS             <1..1824
                     /locus_tag="KRH39_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBG8513680.1"
                     /translation="EKPSDGQMPDGEKPSDSQTPDVEKPSDGQMPDGEKPLDEQTPEE
                     EKPLEEEIVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEEDVKADEQSEINQVFTS
                     EDSFISLWYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTLTFDYVIKGLVGASSK
                     DDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQENQNEEAVKSILYAGS
                     EFDGFGYKQQFNLGEEENDIMTYSATQSAIWIILGQIDEQERLKQYQGSIDLCDKLIE
                     RAKTEEERAEYQKRKEVAINTKAYLEALLKAGREELKPNDTGKPSLSNGLTKINFEKN
                     EDGTYETEAVALVGYSGVVKLKLPDGVTAYDVDGNIIGTGEVEISTQQKFKLKSVGKP
                     DAKANISAVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQNLLSYTIEKKNGQEVN
                     FNIGLPTDDDDVVNPPVPPIDDDVVNPPVPPTDDANGHKPKPSPPIDDTVINPPVSPM
                     DDTIINPPVPPTEDAVLNPPVPPMEDAVLNPPVPPTDDTVLNPPVPPVSDTVEKTPEL
                     SRDDTIVKSPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
BASE COUNT          856 a          278 c          391 g          589 t
ORIGIN      
        1 gagaaaccat cagatggtca gatgccagat ggagagaaac catcagatag tcagacgcca
       61 gatgtagaga aaccatcaga tggtcagatg ccagatgggg agaaaccatt agatgagcaa
      121 actccagaag aagaaaaacc attggaagaa gaaatagtta tagaggaaat gagtttaaaa
      181 caagatattg ataaaattct tgatatgact ttatctcaaa taaataaaat agtatataac
      241 ttttgggaag atgaagaaga tgtaaaggca gatgaacaat cagaaattaa ccaagttttt
      301 acttcagaag actcatttat tagcttatgg tatgacaaaa aagcaaaggt gaaaaattct
      361 tgtttactaa aagaaataga tggttcagat agaccttatc atgatttaag atttgatgat
      421 attacaaaga ctttaacttt tgactatgta ataaagggtc ttgtaggagc aagctcaaaa
      481 gacgacaaat atgtaataga aggtgaagat ggagagcaaa ctgcattttg ttacaataat
      541 catttaagac caccaagtag taatggaaag agtccatatt taccagcaga agattttaat
      601 ggtcaagaaa accaaaatga agaagcagtt aaatctatac tatatgcagg ttctgaattt
      661 gatggatttg gatataagca acaatttaat ttaggcgaag aagaaaatga cataatgact
      721 tactcagcta ctcaaagtgc aatttggatt attcttggac aaatagatga acaagaaaga
      781 cttaagcagt atcaaggctc tatagacctt tgtgataaac ttatagaaag agcaaaaact
      841 gaagaggaaa gagctgagta tcagaagagg aaagaagtag cgataaatac aaaagcttat
      901 ttagaagcat tgttaaaagc aggtcgtgaa gagttaaagc caaatgatac aggaaaacct
      961 agtttgagta atggtttaac taagattaac tttgaaaaaa atgaagatgg tacttatgaa
     1021 acagaagctg tagccttagt aggttatagt ggtgtagtaa agttaaaact accagatgga
     1081 gtaactgctt atgatgtaga tggtaatata ataggtacag gcgaagtaga aatttcaacg
     1141 caacagaaat ttaaacttaa gagtgttgga aaaccagatg ccaaagctaa tatttctgct
     1201 gtatcttatg attatatttt tccaaaagct attcaatatt ataaggcagt gcttgattta
     1261 ggacaaaaag accattcatc ttcattacca tctagcaagc aaaatttatt aagttataca
     1321 atagagaaaa agaatggtca agaagttaac tttaatatag gtttaccaac agatgatgat
     1381 gatgttgtaa atccaccagt gccaccaata gatgatgatg ttgtaaatcc accggtgcca
     1441 ccaacagatg atgcaaatgg acataagcca aaaccatcac caccaataga tgatacagta
     1501 ataaatccac cagtatcacc aatggatgat acaataataa atccaccagt accaccaacg
     1561 gaggatgcag tgttaaatcc accagtgcca ccaatggagg atgcagtgct aaatccacca
     1621 gtgccaccaa cagatgatac agtgttaaat ccaccagtac caccagtaag tgatacagtg
     1681 gaaaaaactc cagaactatc aagagatgat acaatagtaa aatcaccaaa aacaggtgat
     1741 gaaactcagc taatgtcata tgtatttatt agtgtcattg caatttgtgg attagcgtat
     1801 caatgtaaaa taaaaagaaa ttaatctaga ttattattaa gtttaaaata aaaagtctca
     1861 aattgattta taagtcattt tggggctttt ttatgtaaat ataatatgca aatatagttt
     1921 gtttaaaaat atctatttta attatattct taaatttatg attggataca ggcattcatt
     1981 aattgtttga tgaatagaaa aatgttttga aggtgaaaat agtattgaaa aaataatata
     2041 ctaagggtat aattcatttg atttcatttg tataaattta attataaatt ttaaagtgat
     2101 tatataaatt taaa
//
LOCUS       DAEORZ010000283         2111 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEORZ010000283 DAEORZ010000000
VERSION     DAEORZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.289"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(302..676)
                     /locus_tag="KRH39_003817"
     CDS             complement(302..676)
                     /locus_tag="KRH39_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8513681.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            895..1128
                     /locus_tag="KRH39_003818"
     CDS             895..1128
                     /locus_tag="KRH39_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8513682.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            1112..1525
                     /locus_tag="KRH39_003819"
     CDS             1112..1525
                     /locus_tag="KRH39_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513683.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AVFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            1544..1840
                     /locus_tag="KRH39_003820"
     CDS             1544..1840
                     /locus_tag="KRH39_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513684.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITNEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKSYLNRF"
BASE COUNT          731 a          266 c          278 g          836 t
ORIGIN      
        1 tgagataaat tgaggtttat ttgaataaat tgatttgaat tatgttattt tcagattact
       61 tttatttaag tattttgatt ggattcagct gaattaatgt atattaattc ttattgaaca
      121 gtttttattt ttatacatac tatctactat atactataca tattctacta aaatttatat
      181 aaatattaga caaacctatt tcattttctt aacatgttca gaataataaa caaaatgaag
      241 gtagaaaaag ttataatttc ataccttcat tttatttatt atgtatcaat ttgtcatttt
      301 cttatttcaa attcttaaaa tattcttcga gttcacttaa tttatcttca tccacttcgt
      361 cgtcttcacc atgtaacgct gacattaaac ttgaaattga atttttatgt atgttcttca
      421 aaaattcctt tgtttcaaac cttagatatt ttttctcttt tactagtata ttataatgtg
      481 taagtttacc tattttctct gtacttaaaa actcccttcg ctctaacctt ttcaaaactg
      541 taaatgtagt tgattcattc cactcatatt tttcttccat tgcagcaact aattccctag
      601 aagttaatac cttgttagct tcacaaatat atctcataac ttttaattcc gcttctggta
      661 atttactaat tttcataatt cgcgcccctt tgccgattta tgtatatgtt aaattctata
      721 tcaataaagt tttacaaaca agagaattaa ggtaaataat tccatccaat ttttaccata
      781 ttattctttt aggttttata atacttatct aatgagattg acattttact catttcagga
      841 tattatgaag ttaacttcac atgaagttag cttcatatag aaagaggtta tggaatgaaa
      901 acaaaagtac gagaatttcg aacaaatatg ggtctaacac agcaacaact cgctgattta
      961 gtccatgtat caaacagaac aattatttct attgaaaaag agcaatatag cccttctctt
     1021 atgcttgcct atcgtatggc acaagtgttt ggtacaacga ttgaagaact atgttgttta
     1081 aaagaaaata aagaaatgga ggataaaaaa catgaaagta aagagtaaac gaagttttat
     1141 agtgggtatt atagtttgtg tgttatgttg tgctagttta gttatttatt gcattttaaa
     1201 agataaaaga ttcctaattt catcatttct tttaattgta atagcagtct ttaatttttg
     1261 taatgctttt tcaagaaaag gcattgttga agaattacac gatagtacag atgaaagaga
     1321 cttgtactta acaatgaaaa caagtcatat cttagttaaa attatgaatt atacattatt
     1381 tacttttaca tttcttttca tcatagctta tagtgcatgg aaaaatcaat cacttctagt
     1441 tatagctata acactatgtg tcattgagat ttttttattt gttgcatatt tacttatcaa
     1501 tatattttta gagaaaaaag agtaaataca aggggtgata taaatgtatt ttaaaactta
     1561 tatactaaaa agagaagtaa ataacaaaca actagagata actcgaagtt ttgacttaga
     1621 taatctaatt tgtactgcaa cagagatttt gaaagataat gagattgatt tgataattac
     1681 taatgaagta aataatgttg tttggagaaa tgaaaaaagc actcccccag caagagaatg
     1741 cttagttata gctatagaag tgtattggtg gtacacttct atttttttat tatatcatat
     1801 aaattatgtt atcaaaaaga gctatttaaa tcgattttaa ggtgtgttga ataagattct
     1861 tgatagttta tatattgatg aatttcaaaa aaataagcac tctttattat aaggagtgct
     1921 ttggtgtatg ttaatcttct atctaataca attataacat gtcttatgtt ttagtatgat
     1981 aattttcgtt cgctttatta ttacaatttt tactatagtt ttatctatcc tgttaatatt
     2041 tgtaatattt tagatactct ttgtacttgt gttactgata tactttgatt aagaaaagca
     2101 aatttaatag g
//
LOCUS       DAEORZ010000284         2104 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEORZ010000284 DAEORZ010000000
VERSION     DAEORZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.290"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(45..557)
                     /locus_tag="KRH39_003821"
     CDS             complement(45..557)
                     /locus_tag="KRH39_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513685.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     IYNYFDELEK"
     gene            1110..1991
                     /locus_tag="KRH39_003822"
     CDS             1110..1991
                     /locus_tag="KRH39_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBG8513686.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
BASE COUNT          834 a          288 c          200 g          782 t
ORIGIN      
        1 tcaacacaat taccttttca ttcaatcaaa gtaatcttta agcattattt ttcaagctca
       61 tcaaagtaat tatatatgat aacctcacct gtctctttat ctgtagtctt tataatgccc
      121 ttagctccat ttacctcttc ttttgtaaat tcatcatcta gcacaacttt gaatttatct
      181 tcctttccat aaccttgttg aaattcatct ctcaaaattg catttttttc tgcttttggc
      241 ttcaactcaa attcaattcc agatttcatt tcatcaaaat aatttaatac tacaacctca
      301 ttattcattt tattagttgt ctttataatc cctttagctc catttacttc ttcttttgta
      361 aattcatcat ccaatgttat attaaatttg tcttcttttc catattcctc ttgaaaatcc
      421 ctcttcattt cattgtattt agtttcaaac tctttactct tgtttaattc catagctgat
      481 acatttgtca atgatgctcc aattgccatt actgttaatg ccattactat accactttta
      541 aattttttta ctttcatatc ttacctccta aattaaatgt tctcaatgaa attatataaa
      601 gaaaatccta agttttacag tttcattctt gtaactaaaa ttactattat gtaaccaaac
      661 tttttgcttc aactattcct taaaagttgc ttttttgagg aatagttgaa gttaaataat
      721 tagattttat ctatatttat agatttaaaa tcaaatttat ccatataaat aaatacttat
      781 ttttgcaaaa taaaaatttt taattactcc aatccttctt atttacttaa aatcctatga
      841 taagaaattt aaaagtcttt accttcttta acaataattt atttactttt aacatacaaa
      901 atatatagag tatatcaaaa ctattttact gaatattcta aaaataagta ccccctatta
      961 gtatatatat ttacatatta aaaaaaagtt ttaaataaag ttatattttt tacaacagtt
     1021 ttgtaattaa aaacatattg aaggtaaatt taaaggtact atatactata aatatgtcga
     1081 cacaataaat ttaaaaagga gaactaaata tgaaaaaaag attaataata atgatattgt
     1141 ctgttgtatt agtactatca agtatactta cgatatttgc atattcaaat attaaatata
     1201 ataataataa attgatatat tctaatatga tagataaaaa aactcaaaat agtgtaaagg
     1261 aaatcctaaa agaaaataaa attaatgaaa aagacataga tacatttatt aaagcagtca
     1321 acaattataa taaattgcaa gttaaaatac ttcaaaataa tataaatata tctaaatctg
     1381 gatattcttc aatcaatgca aaacaagttc catataacct agaaaaattg caagataatt
     1441 gggtgaaaaa attccctgat tatatggatg taaattgtag aataactgct tttagacttt
     1501 ttaaagattt tataaattca aataagaaat ttactggaga ctctattgac ttgaatgtcg
     1561 acttggacac tataatgaat aataaagatg ctaaatttag caccaaagat gtagaaaaat
     1621 ttattaactt cttttctgca ataccagcta aagacacaga tgatacaatc aaaattgcag
     1681 aacaaataaa aaatgaatgg aaaaaaagaa aaatatcttt taaaaataat aaaaatatat
     1741 ctattataaa tggatttctt cgttatccag aaactaaaaa cgtatttata ggtcatacag
     1801 gcatatgtat taaaactaaa aatggcattc tatttttaga aaaatatggt gttacatcac
     1861 cttatcaagt tactaaattt aaaaataaag aagatgtaaa aaattatatg tttaatagat
     1921 tgaagatgtc agaaggtgaa attgaattac ctgaccctat aataatggaa aacgataaac
     1981 ttatgaaata ataactttaa acgcaaaata gtatttataa ttactaagtc aatataatag
     2041 agaaggtcta tagatttgtg taaatacaag cttatctaca atcttatata agaatgttta
     2101 tata
//
LOCUS       DAEORZ010000285         2018 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEORZ010000285 DAEORZ010000000
VERSION     DAEORZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.291"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            652..1980
                     /locus_tag="KRH39_003823"
     CDS             652..1980
                     /locus_tag="KRH39_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG8513687.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIIIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          737 a          216 c          343 g          722 t
ORIGIN      
        1 acattattta ttttagaaaa aagataatta tctaattata tttaaggata ataatatgaa
       61 aataattttt atattaaaaa atatgaatga aaatagtggg ttttataaaa aaacatattt
      121 agattttaac atctcaaagg agttttcaaa gctaatattt catttttaaa taaaaaaata
      181 aaatattagt tttgatttct ggtttaaatt gggttataat aaattgtcaa attatttatt
      241 aatataaaat agactataaa acaagcaaaa atcatactat tatataaaag accttaaaga
      301 tagaaaacta cagaaagaag ccaaaatgtt tttgaaaaaa atgaaatttt attgcaaaaa
      361 aatcaaaatg aatttaactt aatagttata atctattttt agtgttacaa atatacaatg
      421 tatattatac aatataaaaa atacatttac ttagaagtat tattatattt ggatttttgg
      481 tttttataat ttttttatat ttaaacataa ctaaaaataa aagattacta aagctaagta
      541 gagtttattt tattttaata aataataaag ctatttttct atatattggc ataatatatg
      601 cttatttaac tatgaaatta aaattaaata ggattaatga ggaggaaaaa aatgggaagt
      661 aatgaactga aaaagactct tggcgtatca gcagcattat caactgttgt tggttccgtt
      721 ataggagcgg gcgtattttt taaaccacaa gcagtctata cattaactgg aggagctcca
      781 ggtcttggta tattggcatg gttaatagct ggaatcataa ctataacagc tggtcttaca
      841 gctgcagagg tatcagttgc gataccaaaa actggtggaa tgatggtata tataaaagaa
      901 atttatggag aaaaattagg ttttttaact ggatggatgc aaattgtatt attttatcca
      961 gggatgatgg cagcattagg tgttatattt ggagaacaag catcagcgct aattggtagt
     1021 ccatcacttt tacttccaat agcaattgga attataataa tagttgcagg gcttaatatg
     1081 ttaggttcaa aaactggagg agtcatacaa actgtttcta caatttgtaa gttaatacca
     1141 cttattttaa taatggttgt tggatttatt aaaggtggtg gaaataatcc aatacttaca
     1201 cctatggttg gtgaaggatt aagcctgggt agtgtactag gtcaagtttt aattgctata
     1261 ttatttgctt ttgatggatg gatgaatgtt ggtactttag ctggagaaat gaagaatcct
     1321 ggaaaagacc ttccaaaagc tataataggt ggattgtcag ttgttatggc tgtttatttt
     1381 ataataaatt tagcgtatct ttgggtatta ccagctaatg aattagcaaa ttatgcttct
     1441 cctgcatcag cagttgctga agttatattt ggttcaatgg gtggaaaaat aatatctgta
     1501 ggtatattaa tttcagtgtt tggagcatta aatggatttt tactaactgg ttcaagggtg
     1561 gcatatactt tagctacaga taagacttta ccaaagtatg gtatattttc taaattaaat
     1621 tctgctcaag ttccagcaaa tgcaatagca ttagtatctg ttattgcatc tatatatgct
     1681 ttgtcaggtc aatttaattt attaactgac ttagctgtat ttgctacttg gatattctat
     1741 gtattaactt ttataggggt tatgaaactt agaaagactc atccaaatat accaagagag
     1801 tataaagttc cattatatcc tattgttcct ataatagcta tagcaagtgg tatatttgtt
     1861 gtagttaatc aattgtgttt tgcaggtatg aaaactacta tgatatctat aggaggtctt
     1921 gtaataacag ctataggctt accagtgtat gcttatatga ctagaggagt aaaaagataa
     1981 accaagtagt ttacaaatgc ttcataatta aaataaaa
//
LOCUS       DAEORZ010000286         2003 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEORZ010000286 DAEORZ010000000
VERSION     DAEORZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.292"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>2003
                     /locus_tag="KRH39_003824"
     rRNA            <1..>2003
                     /locus_tag="KRH39_003824"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          542 a          427 c          601 g          433 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tactaccgat aaccgtttga gagaagggat
      781 gacacagtag gataagctaa gcacactgtt ggttatgtgt gcccaagcat tgaggcagtc
      841 aaagtaggca aatccgcttt gataatgctg ggatgtgatg gggagcgaaa tttagtagcg
      901 aagtagctga tttcacactg tcaagaaaag tctctatcga ggttaaaggt acccgtaccg
      961 caaaccgaca caggtgggtg aggagagtat cctaaggcca gcgagagaac tgttgttaag
     1021 gaactcggca aaatgacccc gtaacttagg gataaggggt gccaccatca ggtggccgca
     1081 gagaataggc ccaagcgact gtttaccaaa aacataggtt tctgctaagt cgcaagacga
     1141 tgtataggag ctgacgcctg cccggtgctg gaaggttaag gggatctgtt agagcaatcg
     1201 aagcagtgaa cttaagcccc agtaaacggc ggccgtaact ataacggtcc taaggtagcg
     1261 aaattccttg tcgggtaagt tccgacccgc acgaaaggcg taacgatttg ggcactgtct
     1321 caacaacaga ctcggtgaaa ttgtaattcc ggtgaagatg ccggatacct gcgacaggac
     1381 ggaaagaccc catggagctt tactgtagct tgacattggg tcttggtact acatgtacag
     1441 gataggtggg aggctttgaa accaggacgc cagttttggc ggagccatcc ttgggatacc
     1501 acccttgtag tactgggact ctaaccatag gccatgaatc tggtcttggg acactgtcag
     1561 gtgggcagtt tgactggggc ggtcgcctcc caaaaggtaa cggaggcgct caaaggttct
     1621 ctcagtacgg tcggaaatcg tacgtagagt gtaaaggcaa aagagagctt gattgcaaga
     1681 catacaggtc gagcaaggat gaaaatcgga cttagtgatc cggtggttct gcgtggaagg
     1741 gccatcgctc aacggataaa agctaccctg gggataacag gcttatctcc cccaagagtc
     1801 cacatcgacg gggaggtttg gcacctcgat gtcggctcat cacatcctgg ggctgtagta
     1861 ggtcccaagg gttgggctgt tcgcccatta aagtggtacg cgagctgggt tcagaacgtc
     1921 gtgagacagt tcggtcccta tccgtcgcag gcgtaggaaa tttgagaaga cctgtcctta
     1981 gtacgagagg accgggatgg acg
//
LOCUS       DAEORZ010000287         1999 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEORZ010000287 DAEORZ010000000
VERSION     DAEORZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.293"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            239..523
                     /locus_tag="KRH39_003825"
     CDS             239..523
                     /locus_tag="KRH39_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513688.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(1294..1509)
                     /locus_tag="KRH39_003826"
     CDS             complement(1294..1509)
                     /locus_tag="KRH39_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8513689.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1520..1987)
                     /locus_tag="KRH39_003827"
     CDS             complement(1520..1987)
                     /locus_tag="KRH39_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG8513690.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGCCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          811 a          208 c          245 g          735 t
ORIGIN      
        1 tattaattaa taaaaagata gtagctattt ttatatagct attatttttt tattgttgtg
       61 gatgaaaata tacttatatt ttactaatat acatttaaaa attataattt aaagcattaa
      121 aaaataaatt tgtattttgt tttatataaa gtaatttgaa gaatatggaa aaacttgatt
      181 agtacattat taattagggt ataaaatggt gtaaatagaa tgataagggg tggatgaaat
      241 gcttgtatat aataaaagtt tttatcctaa tgacatattt ccaagattag atttttcaaa
      301 aataaaaaaa cagttaaaat tgatagataa tgacctgtca gattttggaa gcatatgtat
      361 aatagaaaaa gaacattata caataagtgt aaacagtata ggtgaaataa atgtgtatta
      421 tgatttagag tacgaaaata aggtgtatag aatagtttat gagattgaaa agttatttaa
      481 atctcaagtt ggaaggttta gcatatctac atacagaaat tgataattaa aaaagtagaa
      541 attaaaaaac ttaatactaa agatatagat aaatatatga ggtaacagct agagagagtt
      601 gttacccttt ttaaatacta agtatatatt attcttatta attttttgta aattcactaa
      661 aagacctttt tatagaatat tattttcttt ttttattaaa taggtaaatc agtaaaaatg
      721 caattccagt tccaattatt attccaatga tattttctat aattaatttt tttaatataa
      781 aagtaaataa taaaaatgct atacataaac ctaaaatata tcctttaaaa taggttgaaa
      841 gttttctttt catataatca ccaacttgta tcagatttag tttacttaat tagagtataa
      901 cagtgttgta acttcaaaga gtatctaaaa gattacaaat gttagataaa atcatcaata
      961 atgtaattta taacacgtag ctataaacta tgcaataata taaatggagg tataagtaaa
     1021 tgaaaaagtt ccatgttttg ctaacagttt aaacaaatat taggtagaaa agaatgtata
     1081 taggaatctg tggtaaaatt atcaatttaa aagtatgatt tagtgtgata cagttcttat
     1141 aggtaatgga taacaaatag ataaaggaac agtttctttc ttagaacata ttttgttatg
     1201 actttaaaca aagttaatat taatgtcaat aatatagtaa aagaataaaa atcatatagt
     1261 ataataaaaa agagtgtttc taaacactct tttttaatca ttttcatctt ctacatattc
     1321 aagtatatca cctggctgac agtctaaagc tttgcaaatt tcgtttagag tggtaaatct
     1381 aatagctttt gcttttccag tttttaaaat tgataaattt gcatttgtaa tatctatttt
     1441 ttctgccaaa tctttaagtg atattttcct ttttgccatc attacatcta ggttaactac
     1501 tattgccata acatcacctc tatattgtta aatcattatc ttcttttatt tttatagctt
     1561 tatcaaatgt ttctgcaatt acaaagcaca ttagtgcaaa tataaaagag ataaccataa
     1621 gtggtgagat accgccacta tcagttccaa aaattcttat attattagta tttaaattat
     1681 aaccatttat acattcaatt atagtgttta tcaataaaca acatcccatt accttaaacc
     1741 tttttacatt ttcaagtacg aatggggaat acttagtact attaattata aatatcaaga
     1801 ataagatgac tataaagtag tcagaaaaat atagtattct tgcaattaaa gcgattactt
     1861 tgtcagaact aaaatcatat aaaaacatag gtatcataca taagaatatt ataccagcaa
     1921 ctcctaaaag tgataataga aaatatagaa atactgataa tatattacta aaaaattttg
     1981 taatcataaa atatcatct
//
LOCUS       DAEORZ010000288         1968 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEORZ010000288 DAEORZ010000000
VERSION     DAEORZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.294"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            26..235
                     /locus_tag="KRH39_003828"
     CDS             26..235
                     /locus_tag="KRH39_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513691.1"
                     /translation="MMGLKLKRRYKNVLIYINIRTIININCKIKFIELYLTFNELKNK
                     LANNEKTTVINENNKAFSGLVDFSN"
     gene            complement(604..987)
                     /locus_tag="KRH39_003829"
     CDS             complement(604..987)
                     /locus_tag="KRH39_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513692.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
     gene            1114..1443
                     /locus_tag="KRH39_003830"
     CDS             1114..1443
                     /locus_tag="KRH39_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513693.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYLEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVF"
     gene            1640..1849
                     /locus_tag="KRH39_003831"
     CDS             1640..1849
                     /locus_tag="KRH39_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513694.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVRGGSSWSFNFIV"
BASE COUNT          743 a          235 c          254 g          736 t
ORIGIN      
        1 atatccagta ggttaatttt atataatgat ggggctcaaa ttgaagaggc gatataaaaa
       61 tgtattaata tatataaata taagaactat aataaatatt aactgtaaaa ttaaatttat
      121 agagttatat ttaacattta atgaactgaa aaataagtta gcaaataatg aaaaaacaac
      181 agtaataaat gagaataaca aagcattttc aggattagtg gatttttcta attgagcttt
      241 aatcctagtt tgttcaattt cgatatttct aatattatta gtattatcaa tttatttgta
      301 ataattatca gtttttgcga aatatttttg ataatttata gaactttata tattttcctt
      361 gaagtatttt tcgcccttat tgtttggttt ccaattttaa aactcataaa ctcccaataa
      421 attcatgtat aatatacctt aatttataat atatttaaga gtttatttca gtaatttttc
      481 gaacgatttt gatatatttc atttatagtt ttcaaaaact tatacaaaaa agagtgtttc
      541 atttttctaa acactctttt tataagaatt tgaataataa tttttttaaa attgataata
      601 tggttatttc tttttcaagt ttatagtttc ctcatttcca ttccaagata taattagctt
      661 gtaactagat tctctatttg aataaagacc tggtctttgt atttttattg ctttttcttg
      721 acctgcttct aaaatttcat tttttacaac tacaactgca tcttcaccat ctataaattg
      781 ataggaataa ttaaattttt gttcaactac atctttacta tttttatatt ttatttctaa
      841 ttttgagtgt ccttcagtag ttacaaattt agctttccaa ttttctgatt ctccaataaa
      901 gacattttta tcatttttta tattaagtat agctatcaca gagattatta aaactataag
      961 aatacctata aaaaattttt tcctcatatc aatccctcct ttgaataatt ataacatgag
     1021 aatagttatt caatactaat tttaaaaata ttgtggattt atattctatt acaatagaat
     1081 gtctgttata acaaataaaa agtaggggtt aatatgaatt ataggttaaa aaaatctgta
     1141 acaactattt tatctttact cataatgaca atactattac caacaaatgc aagtgctagt
     1201 aaggtagaag attcatctat gaaagtaatg tatttagaga aacaagtaac agacatggac
     1261 aaattatacg atatggctaa aaatggaatt actgatgtta aaagttccaa tgaaaaaggt
     1321 ataatcaaaa atgcaagaac aggagagtcc atatacgttg attctatatc aacaactcaa
     1381 ttattagaag ttaaacaatc taaaaattta acatcaaaga tatattctaa gacagttttt
     1441 tgatactaat acaataaagc ctcttagaaa agcaagtata tcagaagatg gatgggataa
     1501 aactggtggt gtaaaagcat ctggtacagt tacatataca atatcaaaag gagatggagc
     1561 atttcaatat atttccgtta gtaaagtttc tggaaactgg tctattgcag acggccagat
     1621 tcgtgtaaca aatagaaggg tgcatataaa tcaaactgga tttatttcta atgggctagg
     1681 aattaagcag caacaaaaga aagagccaac atcaaattct ttctcatata atgcaccttc
     1741 tagttggaaa ccagtaggta gagattcagt tcaatacaat tgtgggtttg aattaattgc
     1801 aacattagta agaggaggaa gttcttggtc attcaatttc attgtttaac cttaaaatat
     1861 tttaagtgaa ttttattaaa attaaagata tacatatact ttaaaattag aaaatgtaaa
     1921 ttaatttgtg tttttaaaga taatttctaa gttatagcaa caattgct
//
LOCUS       DAEORZ010000289         1843 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEORZ010000289 DAEORZ010000000
VERSION     DAEORZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.295"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..481
                     /locus_tag="KRH39_003832"
     CDS             <1..481
                     /locus_tag="KRH39_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513695.1"
                     /translation="NIISNIEYIYKLNINEDGIYNIILHWISEYDYNDIDTLTKINNL
                     EIIFTVIEKVVRGLFVEMLCKLQNICKINDSTKLHNKLQYIKDILYNGLKENIFLEKL
                     NYKLLDRRLTKKINLYMKKNAELYESRVETILLNIREYVDLDEFELFIIDKLIQKIK"
     gene            626..763
                     /locus_tag="KRH39_003833"
     CDS             626..763
                     /locus_tag="KRH39_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513696.1"
                     /translation="MNNLNKNTILGEVIITITACDDEKEIQIVIKNQVDNILKNKNKE
                     Y"
     gene            1095..1340
                     /locus_tag="KRH39_003834"
                     /pseudo
     CDS             1095..1340
                     /locus_tag="KRH39_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
     gene            1334..1606
                     /locus_tag="KRH39_003835"
     CDS             1334..1606
                     /locus_tag="KRH39_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513697.1"
                     /translation="MLNHINKILLILSLFTPIMILKSILTYDKDTDKTKKISFFTVLL
                     AYLITKIIILFNVDKTHDYNMKKNLYDIIYYLIFITWELTIIIFIR"
BASE COUNT          846 a          160 c          222 g          615 t
ORIGIN      
        1 taatattata agtaatatag agtatattta taaattaaat ataaatgaag atggcatata
       61 taatataata ttacattgga taagtgaata tgattataat gatattgata cattaactaa
      121 aattaataac cttgaaataa tatttactgt tatagaaaag gtagtaagag gtttgtttgt
      181 tgaaatgcta tgcaaattac agaatatatg taagatcaat gacagtacaa aattacataa
      241 taaattacaa tatattaaag atatattata taatggactt aaagaaaata tatttttaga
      301 aaaactaaat tataaactac tagatagaag gttaacgaaa aaaattaatt tatatatgaa
      361 aaaaaatgct gaattatatg aaagtagagt ggaaactata ctactaaata ttagagaata
      421 tgtagattta gatgagtttg aattatttat aatagataag ctaatacaaa aaattaaata
      481 ataaatctaa tcgtaggtgt tatccctatg aatcgtatcc accattgaaa tccacgaata
      541 cctatatcca attggatata ggtattttta tacattttta gtttaaatat tattgataaa
      601 tacatttaaa aagttttaac ctaaagtgaa taatctaaat aaaaatacaa tactaggaga
      661 ggtcatcatt acaataacag cttgtgatga tgaaaaagaa atacaaatcg taataaaaaa
      721 tcaagttgat aatatattaa aaaataaaaa caaagaatat tgaattaaaa aaatatagct
      781 caaaagaaga accattacaa agttaattga gaggatacac tactcttaga tataaaaatg
      841 gcagaactaa atagtataga tatcgcaaga aacataagaa aagtagataa taatatttat
      901 aacatctcta ataaattatg tacaggaaag atatgaagta agagcttatt gatacttatt
      961 aaagtcaata aattttcaag aactagaaaa acacatatta acatgtatca aaaaaattga
     1021 aaaaaataaa aataagtcta acacatacaa aatctactct agcaaaaaaa tagatagaag
     1081 ttcaaaaaaa gaacatgcta atacatacaa aaaataaaac ttttgatgct atatgtatac
     1141 tagaaaaaat agaaaaagat ttaaatatag aggaacttgt aagatgtcat aaaagttata
     1201 taattaactt aaactatgta gaaaatataa agtcaaatac tgcaatatta gacagtcaag
     1261 aataagtacc tattagtaga tatagatata aagatataaa agagaggttt ctaaaattta
     1321 ttggtgatag aatatgctaa accatataaa taaaatatta ttaatattat cattgtttac
     1381 accaataatg atactaaaaa gtatattaac ttacgataaa gatacagata agactaagaa
     1441 aatatctttt tttactgtat tattagctta tcttataact aaaatcatta tattatttaa
     1501 cgttgataag acccatgatt ataatatgaa gaaaaatcta tatgatataa tctattattt
     1561 gatatttatt acttgggaac ttactataat tatttttata agataggaat tgttaaatat
     1621 atagtttcat ttttcttatt tgacgtatat caccctttaa taaaagatgt tatatctata
     1681 aatttatttt ttgtagttac aagtaatgta atatcatcag aggaagctct attagtgttt
     1741 agttatgata atgctaaagt agatatatac tattatctta taataataat tattatatag
     1801 tagcttgtgc tatatttaaa tttattaata aaagattggg act
//
LOCUS       DAEORZ010000290         1842 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEORZ010000290 DAEORZ010000000
VERSION     DAEORZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.296"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(66..200)
                     /locus_tag="KRH39_003836"
     CDS             complement(66..200)
                     /locus_tag="KRH39_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBG8513698.1"
                     /translation="MWYKIIKKYYDDKLWSKEQVKTAVIKNKITENEYKNIIGEDYIV
                     "
     gene            complement(288..407)
                     /locus_tag="KRH39_003837"
                     /pseudo
     CDS             complement(288..407)
                     /locus_tag="KRH39_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(421..1443)
                     /locus_tag="KRH39_003838"
     CDS             complement(421..1443)
                     /locus_tag="KRH39_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513699.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
BASE COUNT          630 a          280 c          160 g          772 t
ORIGIN      
        1 acaagtataa agctttttag taactctttc tttaaaaata gttactatat aaaattaaaa
       61 aaggactata caatataatc ctctccaatg atatttttat attcattttc tgttatctta
      121 ttttttatta ctgctgtttt aacttgctct ttagaccaca atttatcatc ataatatttt
      181 tttattattt tgtaccacat ctatatcaac tccttttcaa tcattgcaaa agttaacgct
      241 gctgtttctt ctcttaatgc ttttacttct tcgtgtgtag ctatatttcc tgctccattt
      301 ggtcctagaa gaccataaac tccattttcc agagttactg taatcttgtc gtttgctact
      361 tttttaccat atatctttga aagattttct atttctaatt tattcatctt aactcccctt
      421 ttatctactt atataatctg attttattac taaaaaacat aatgacatag cacctatcaa
      481 tattgccatc aaaagtccta ttgctatatt gacaatgtct atattcactt taaatatcat
      541 aatatcacct agataatttc ctattatttt attaaaacca aatatcaaat cataataatt
      601 tacatttgat acaactgaaa gtaatttttt aggtaatata tggattaccg acattatctt
      661 agttatagat ataataaata taaaaaaact tattgaagat attgaagact tacttataaa
      721 agacccaaat atggctatca tggcccatcc ttcaaacatc aaaagctcta tgcctatatt
      781 taatattaca tactttgtta tacttaaatt agttttcaat gcaactacat tgtctactat
      841 tctaaaagat tctaagtctc cattaacagg tactccttgt tgcatacaag ttataataaa
      901 tattcccatt aaataaacag aataaattac taatggaact aataaagcaa caaatatttt
      961 agactttaat gcttttttct tctcttttga tgatagtatg atatctttta ccgaagtaac
     1021 gacttcatct acgtaaatat tggatataat tatagatata atgattaaca ttcctacatt
     1081 tataagtgaa tttgtaactc tatagttaaa tactttccaa aaactaatat ccttatattc
     1141 attcacactt agattatcaa gttttttctt tgacattaat tcaatttttt tagaaaattc
     1201 acctgtatct gtttgatttg aaaactgctt taaaacatta atcttatttt gaaatttctc
     1261 attacctacg tcgataccac ttctcgtatc ttctatacgt cctttttctt tatctatata
     1321 ataactttct gtttctaaaa caggcgtaat aaagatacta atcattagta ttaacactaa
     1381 tatccctaga gatataaaca tactcttaga tttagccaat ttttttattt cccaatttac
     1441 caatatattt ctcctcctaa atcattttaa ttacacatat atattaaaac ttaaatctta
     1501 tataaatata atactaacct taaataaagc ttaaattatt ttataattta taggtgtgtt
     1561 tattattgga gttacattta ttatttttct gggaaattat acattttcaa ttatttttat
     1621 tatttttgat atttttattg attggaaatt ttattgttgt tcaaattgta gttattcttt
     1681 aaatttaaag atggagtttt ttaagaatag aacctactgt ccttattatg gaaagattat
     1741 taaatagttg catataactt tatttatctt gaatcttatt tttaaaaaca attataattt
     1801 tagaatattt tctattctca aacaagcatg ttgaataatg aa
//
LOCUS       DAEORZ010000291         1791 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEORZ010000291 DAEORZ010000000
VERSION     DAEORZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.297"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            142..1740
                     /locus_tag="KRH39_003839"
     CDS             142..1740
                     /locus_tag="KRH39_003839"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBG8513700.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
BASE COUNT          685 a          220 c          278 g          608 t
ORIGIN      
        1 ttgtagtatt tatgtaagaa ataaatttta tataatattt tttcactaaa tttttttctt
       61 tttacaacaa atactcacta ccttttgata taatattttt atcaattatt attatattca
      121 agttttgaaa ggagtattaa aatgagttta ttagttttcg gacataaaaa tccagataca
      181 gattcaatat gttctgcaat ttcattagca catttaaaga acaaactagg aataaaagca
      241 acagcttatg ctttaggaga tatcagaaaa gaagctaaat atgccttgga ttactttaag
      301 gtagatgcac ctgaaatttt agataatgtt agaatacaag ttagagattt aaattatgat
      361 aaagtagtac ctttaacacc aacttcatct atattagagg cttataacct tatggatgaa
      421 aaaaatgtta aaacactacc tgtaacttat gatgatggaa cttttgctgg tatcattact
      481 atgaaagaaa tagctaaaaa tctacttcat caacacttta caactataca cacatcacta
      541 tcaaacattt gtaaaaacct taatggtact attcttgtgg actgtggtga agatataaaa
      601 ggtagagtag tcactttatc atttggaatg gagactctaa tagagacttt aaaagaagga
      661 gacatagcta ttgttggaga tagatatgat agtattgaat atgctattga tacaaaagtt
      721 aaacttttaa tacttactaa tcatacagag atttcaaaag atttgctagc tcttgctaag
      781 ataaatggag tgtctgttgt ttctgttgaa agtgacaatt ataaaacttc taatgtaata
      841 aatcaatgta gtttcttaga ctctatagaa gctacagaaa atttaattac attcaaagaa
      901 gatgattata tggatgaaat aaaagagatt atgctagaaa caaacttcag atcttatcca
      961 atattagatg atgataataa gtttttaggt ttagtttcaa aaggtcatct attaaaccca
     1021 tctaaaaaga atgttgtttt agttgaccat aacgaatatg ctcaaagtgc tgatggaatt
     1081 gaacaagcaa acatagttga aataattgac catcataaac ttggtggtat ttctactgat
     1141 gttcctatgt catttagagt tatgcctgtt ggatgcaata gtactataat ttatcaaatg
     1201 tacaaagaaa ataatgttga gataccttat gaaatagctg ggcttctttt atctgctata
     1261 ttatcagata cattattatt taagtctcca acaacaactg atatagataa gaaagcttgt
     1321 gaagaattaa gtaaaatagc aaaagtagac atggaaaaat acgctatgga tatgtttaaa
     1381 tgcggtactt ctttagatga atatactata gaagaaatag taaatatgga ctttaaagaa
     1441 tttaatatga gtggacatag agttggtatt ggacaagtat ttaccttaga catagactct
     1501 atatttgcta aaaaagatga gtttttatct tatataaatt cgactgatta tgacaaacta
     1561 attcttgcgg ttacagatat aatcaaagaa ggttcttatt taatctataa agcagaagac
     1621 agcttaattg ctaacgcatt tggtgttgaa ggtgttcaag gtacatttgc accaggtgtt
     1681 gtatcaagaa aaaaacaatt agtccctaat ttaactactg caatcaaaaa ttttaaataa
     1741 tataaaattt ttaaataacg aaaactgtct caaatctata tttgagacag t
//
LOCUS       DAEORZ010000292         1702 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEORZ010000292 DAEORZ010000000
VERSION     DAEORZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.298"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..646)
                     /locus_tag="KRH39_003840"
     CDS             complement(<1..646)
                     /locus_tag="KRH39_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513701.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLAGILITRVFFVIDNNK"
     gene            complement(672..1373)
                     /locus_tag="KRH39_003841"
     CDS             complement(672..1373)
                     /locus_tag="KRH39_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG8513702.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKAALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
BASE COUNT          598 a          325 c          216 g          563 t
ORIGIN      
        1 ttttattgtt atcaattaca aagaagaccc ttgtgataag tatcccagct aacacaataa
       61 acaatgttaa taatccacct ggtataccaa ttttttcacc aaagtacata attgctaacg
      121 ctacagcaat cgtacatcca ccagctcgaa tatccttacg tgcaatatca acataaattg
      181 ctccaaataa tgctggaagt atataattaa atgaattagt aacaaattca ggtaacaaag
      241 gaattacttt atagcctaca atggtaaaaa tggtaatcat agaagctgat acaaagattg
      301 agcaacatgt accaattgtt ccaacaactt caccctcatg agttcctgct tctacacctg
      361 caattttctg tgccattgat gctgcaggca tacgtatatc tccaacagaa cctgcaagcc
      421 aacctatata agaacctgga gctcctagtg tcggataata agctataggt tgaacaatcc
      481 aactaacccc ataagtcgca gcgactaacc cccaaatttg aaatataaca gaaagcattg
      541 gcatctgacc aaagcgaata cctacataga tagcgggaat aaagtttgca ataattgcaa
      601 gacctataga aattctccct atttttatca tctttttatc atacataatt atcacctctt
      661 atctgtttta atcacgataa tataatctgt gtaccaaaca taccaattaa catggcaata
      721 cctaaggata attcttgtaa tcgttgattc tttttaaata aagttgtcaa tactagcata
      781 actatagctg aaataattgc tggtgtaagt tttggtattt caaatgtttt tatttgattt
      841 gaaagaaggt aagcaaataa tccaactgta gctcctgcaa gtagcttttt aacccaagta
      901 ggattatact tcatgttcat cttttcaaca attccacgca ttctatgacc caaaataaat
      961 acaacaaaca gccaacctaa gttatttaaa gccattcccc atagtgcata tgaaaaagct
     1021 tctgctccaa atgcagcact acctacttct accccagata aattggatgt aagagatatt
     1081 actccaagct ctgttctagc agctcccaca tcacagagcc tcatccaagt cgtaggagca
     1141 ccaatcacag ctaccagtga taataatgta ataacaggag atagagaagg tccaattgct
     1201 gtaacactag ctgagcgaat tgctgcttta attcctttcc tttcaatacc aagatttgaa
     1261 gcctctttta gcgccgcttt cataaatacg attgcttgaa caactgaaat aattactaaa
     1321 aaactagaaa caatccaaag acctgggcta ttaatcattt cttgaaactt catactttat
     1381 tcctccttaa aataaatttt tcagcatttt attgtgagct ttctttctgt aatcttgtta
     1441 tgtttttata ttgttttaat ttattttatc taagatatag tatctttaat aggagattat
     1501 ctgtcttaaa gtctcactaa aaacattaat acttaaatat tttattcata attagtaaat
     1561 atcagaaaat tcacatatta tttttttaac ctgtaattaa cttataaggt cttttattat
     1621 tttgacattt caatataaaa attttaatca aaaaacttta ttattttaaa aaaaattatt
     1681 atttttataa tttatatttt tt
//
LOCUS       DAEORZ010000293         1701 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEORZ010000293 DAEORZ010000000
VERSION     DAEORZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.299"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            164..1600
                     /gene="miaB"
                     /locus_tag="KRH39_003842"
     CDS             164..1600
                     /gene="miaB"
                     /locus_tag="KRH39_003842"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBG8513703.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT          726 a          171 c          304 g          500 t
ORIGIN      
        1 ataataatta tatgatttta tattttagaa atatagtata tataaatcat tagatttact
       61 attaaaacac aactagaaaa ataataatat ctttaaaaga tttttcttat ttgatataat
      121 taaggttatg tgtaatataa gaaattaact aggaggaata gaagtgagca aaagaaaaca
      181 agtaacaata cctatagaga aaatacaaga acaagataaa tatatagaac aaatacacag
      241 acaaaatgaa gaatacttca acagaactgg aaagagaaaa ctcgtgttta ctcaaacctt
      301 cgggtgtcaa atgaatgaac atgattcaga aaaattatgt tcaatgttag aagagatggg
      361 ataccaaatg tcaatgatgg ttgaagaaag tgatttaata atatataaca cctgtgcagt
      421 tagagaaaat gcagagctaa aagtgtatgg taacttagga caattaaaac atctaaaagg
      481 aaaaaatcca gatatgaaaa tagctgtatg tggatgtatg atgcaacaac cacatgttgt
      541 agaggaatta agaaaaaaat ataaacatgt agaccttata tttggaacac ataatctata
      601 taaatttccg caattactta cagaatcaat aaattctgac aaaatgttag tggatgtatg
      661 ggatgtggat ggagaagtaa tagaaggcct tagaagtaat agaaaatttg aattaaaagc
      721 atttgtaaat ataatgtatg gatgcaataa tttctgcaca tattgtatag tcccatacac
      781 aagaggaaga gaaagaagta gaactcctga agatataatt aacgagataa aagagcttgt
      841 tgcaaatgga acgaaagaaa tcactttact tggacaaaat gtagattctt atggtaagac
      901 acttgaaaat ccagtttcat tctcagaact tttaagaaaa gtgaatgata tagaaggaat
      961 tgaaagggtt cgatttatga catctcatcc aaaagatata tctgatgaag taatatacgc
     1021 aattagagat tgtgataagg tgtgcgagtt tttacatcta ccaatacaat gtggaagcag
     1081 tagccttttg aaaaaaatga atagacatta tacaaaggaa tattatttag aaataataga
     1141 aaaagctaaa aaggaagttc caggtattgc tttttctaca gacttaatga ttggtttccc
     1201 aggagagaca gaagaagatt tattggacac tttagatgtg gttgaaaaag ttagatatga
     1261 ttcagctttt acatttattt attctaaaag acagggaact cctgctgcta aaatggaaaa
     1321 tcaaatacca gaagatataa agcatgatag atttaatagg gtgttagaag ctgtaaatag
     1381 gatttctgca gaaataaatg atggatataa agatagaata gttgaagttc tagttgaagg
     1441 aagaagtaaa aataatgaaa ataagtttgc tggtagaact agacaaaata aactcgtaaa
     1501 tttcgaaggt ggaaatgatg atttaattgg taaattagtt atggtcaaaa ttactgaacc
     1561 tagaactttt tcattaaatg ggatattagt aaataattaa tattgtacaa aataataaaa
     1621 ttatgtaaac taatcaaaat agatgtacta tagaacaaat tatattaaaa atttttattt
     1681 taagcgaatt tgtcatatgt t
//
LOCUS       DAEORZ010000294         1686 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEORZ010000294 DAEORZ010000000
VERSION     DAEORZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.300"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..192)
                     /locus_tag="KRH39_003843"
     CDS             complement(<1..192)
                     /locus_tag="KRH39_003843"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8513704.1"
                     /translation="MLETYVFWIILTIVSATIEWTVLKFILDEISELRNKKLVLNSSL
                     LLAIMTITILTLVEFNINIK"
     gene            complement(186..896)
                     /locus_tag="KRH39_003844"
     CDS             complement(186..896)
                     /locus_tag="KRH39_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8513705.1"
                     /translation="MYRIVICEDDITQITFLRECILKSLEGISSQIELFEFNSGEELL
                     ESNLESIDIFFLDIKMLKITGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKRRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLQYIQFICKNAVVINNEDIPVSKY
                     RISDLKTKLTHVLGDVLC"
BASE COUNT          637 a          241 c          136 g          672 t
ORIGIN      
        1 tttaatattt atattaaatt caactaaagt caaaatagta attgtcataa ttgcaagtaa
       61 cagactactg tttaagacta actttttatt tcttaattca ctaatttcat ctaatataaa
      121 ttttaataca gtccattcta tcgttgcgga aactattgtt agaataatcc aaaatacata
      181 tgtctctaac ataatacatc gcccaataca tgtgttaatt ttgtctttaa atcagatatt
      241 ctatatttac taactggaat atcttcatta ttaataacta ctgcattttt acatataaat
      301 tgaatatact gcaaattaat taaataactc ttatgacatc taaaaaaatt atatttttct
      361 agctctaatt ctattttatc catactattt ttagtataat aagtatcatt tttagtatgt
      421 atagtcaaaa cctttttcct aacctctata tacataatac tattaattaa aattttattg
      481 attatacctt tattttctat caacataaaa ttttctcttc tttttataat atccgatata
      541 cagcttaaaa tactttcatt taaatctcca aaatctatag gctttaataa atatctataa
      601 gcccttacct tatagccttc ctgtatataa tcgactattg aagttataaa tattatttca
      661 gaagtatcat tagtttctct aattatttta gccacatcca tacctgttat tttaagcatt
      721 ttaatatcta aaaagaatat atcaatactt tccaaattag actccaatag ctcttctcct
      781 gaattaaact caaatagttc aatttgacta gaaataccct ctaaggactt taatatacac
      841 tctcttaaaa aagttatttg tgtaatatca tcctcacaga taactattct atacataaac
      901 gaccctcctt agacattaga tataaaacaa ataaaatatc acatctataa ataactattt
      961 agttatacaa ctaacaaatg gattaccaca tataaactcc aatatttatt tactatctct
     1021 atccctaaac ttaatgattt agctacctac tttaaattag ctcataatct ttagtatttg
     1081 tgtataaaca tctgtacttt cattgtacaa atagtcacaa aatgctgtta ttcacataaa
     1141 aaatagacat ctatttttag tatgttaaca gcaaaaataa gaagatagct tagattttta
     1201 aaaacgattg atacttaata tagttttgta tactatttat aggtaatttt ttatattatt
     1261 ttaaatatta aaattattga tgtttaatga ctattatatt gatattaata cgtaattaat
     1321 gatataaaaa attttttaac taaagctctc tatctaagat ttttctatag aaaagtctag
     1381 taataaatcg aaaatgaacg gctgaccttt tatattttgt tttcgatcta tttacttgaa
     1441 ttttaacata aatctatctt ttagtatata ttaatcatgc taaatttgta ataacatgta
     1501 tacaaattat tgcttatata ttttttaata aaaaatatta ctgggattaa aaatctccag
     1561 taatatttaa taagcaaaaa acatgtctat acaagtatat aatttagttt aattaggtat
     1621 taaattattt aaccagatta gtaaacttaa gcattagtct accaattgtt gatatagcac
     1681 ttaagt
//
LOCUS       DAEORZ010000295         1630 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEORZ010000295 DAEORZ010000000
VERSION     DAEORZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.301"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(118..1461)
                     /locus_tag="KRH39_003845"
     CDS             complement(118..1461)
                     /locus_tag="KRH39_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG8513706.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(1534..>1630)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT          598 a          275 c          240 g          517 t
ORIGIN      
        1 caaagttaat agcaaaatca atttacatat aaagtgattt tgctatttat aaatttaaaa
       61 taaataagat tttattctat ataatttaaa atattgacct agaactattt aaaaatatta
      121 acttagaact aagtttacaa ctgcaagtaa acctataatc caaagtgtaa tactgatttt
      181 atcagtttct ttagctacta catgcattat tatatatgaa ataaatccaa agcataaacc
      241 aatacttata ctataagtta aaggcatcaa taaaactgta acaaaagcag gtacagctat
      301 tttgctatca gcaaaatcaa tctgttttat gctttcaaac atgaatacac caaccattac
      361 aagtgcagga gctgcagcgt aagtaggaac tataccaagt aatggtgtaa atataagtgt
      421 agctaagaat aatgctgctg ttactattga agcaagtcct gttcttgcac ctgctgcaat
      481 accagaagca gattctccaa atgttgtaac agtactagtt cctaagaatg agcctattaa
      541 agtagaagaa acatctgcat ataacattct tcctaaatct tttatttcac cattctcatc
      601 ttcaagaccc atttgtttac aacaagtaat aagaaatgct aatgaatcaa acatgtctat
      661 aaacatgaaa gagaatatag gtccaattaa tgagaatttc aatgcaccta caatatctaa
      721 tttaaaagca actggtgcta tactaggagg tgttgagatt attgctgttg gcaactccac
      781 aaatccaaat ataacagata ttattgttgc aactactata cttatcaata ttccaccttt
      841 aactttttta tattctagta ttgccattaa gataagagca aaaagagcta ttaaaactgt
      901 tggtgtaaaa tctgcaagtg aaactatagt atctggatta gctattataa cacccatatt
      961 ttttaaacca acgaaagcaa ggaaaagacc tatacctgct gaagatgcta ctgccaattc
     1021 tctaggaatt gcgtctgcaa ttgtctttcg aagacctgtt actgcgagtg taaagaagac
     1081 acatccagat agaaaaacta ccccaagacc tgtttgccat gggattcctg cacctataac
     1141 caaagagtat gtgaagaatg catttaatcc cataccagga gctagagcaa atggtgcatt
     1201 tcctaaaatc gcaaatataa gtgtaccaat ggctgtagct agtatggtaa ctgttataag
     1261 ggcggcttta tccattccag cttcagataa tattgctgga tttacgaaaa ctatgtatgc
     1321 cattgttaag aatgtagtaa ctccacctat tacttctgtt ttaaggctag agccacgttc
     1381 gctaaattta aacattttgt ctaaaaagct taatgatgaa ttatttccac ctggttgtac
     1441 attgttagtg tttggtacca tttttacctc caaaaaactt taagatataa acaaaaaaat
     1501 atatattcat atagatagaa tagaacatat attcagagtt gacattctat tcatagaaaa
     1561 cccatttaag gtgagtttgt agaaactatt agaccgtatt actaatatta tatgaacatt
     1621 ttctatttgt
//
LOCUS       DAEORZ010000296         1567 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEORZ010000296 DAEORZ010000000
VERSION     DAEORZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.302"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            105..1553
                     /locus_tag="KRH39_003846"
     CDS             105..1553
                     /locus_tag="KRH39_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBG8513707.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETYARLKN
                     VSKVYIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVML
                     TNAYKGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNT
                     KSTRLGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGS
                     NKSILKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVG
                     KDISAGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIED
                     STMTLAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEG
                     IVTNDTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          644 a          164 c          268 g          491 t
ORIGIN      
        1 acttaatata atttgcaatt ttacataagt ttgttaagta cataactaat ttaagaatat
       61 gtatttatat attaaaaatt tattatgagg gggacaattt tattatgaaa ttctataaaa
      121 gaatattaac attgacttta agtatttctt ttgtatttgc aaatattcaa ttagtagatg
      181 caatatcatc tgtagaaaaa atacaaggaa aaaataagta tgaaatagca ggtaaaatag
      241 cagataaaaa tgcatataaa actgcatata aaactgctat attaattaat actagtaact
      301 ctattgcaga tggattaagt gcaagtggac tagcaggagc tttaaatgcc cctatactat
      361 taactgaaaa aaatactata ccaactgaga catatgcaag attaaaaaat gtatcaaaag
      421 tttatataat tggaggaaca tattctatta gtacatctgt cgaaaattca ttaaaatcta
      481 agaaaatgaa agttgtaaga attaagggaa atgacagaat aaaaacaagt tataatgttg
      541 caaaagaaat aaattcaata aaaaaagtca atactgttat gctaaccaat gcatataaag
      601 gagaagcaga tgctataagt attgcatctg tagcagcaag agataaagca ccaataattc
      661 ttactaatgg acaaagtata cctttttcaa ctagtggttt aaagtcttat gtaataggag
      721 gaacagcttc aatgagtacc actttagtaa acaatactaa atctactaga ctagggggga
      781 gtactcgttt tgaaactaat aaagcaataa taaataagtt ttataaggat gctagagagt
      841 tttatatagc tggagcttat gaattaacaa atgcacttgt aggatcttct ttatcaaaac
      901 atggtccaat ggttttagtt aatgatggta gcaataaaag cattttaaaa aatgctaaga
      961 agataacttc tataggatat atagattcaa atatagtaca acagtgttta aatataacaa
     1021 atggaattgg agatattaat actggggttg taaaaaatgt taaacctact acaaagacta
     1081 taaaagatgg aatgtataag gttggaaaag atatatcagc aggtgaatac ttaataactt
     1141 caaactcagg aagttatgct tcttattatg aagttacaag tgatagtaca ggtaatgctg
     1201 atagtatact ttcaaatgat atttttagtg ggactagata tatcacctta aagaatggtc
     1261 aatatattaa aattgaggat tcaacaatga cattagcaaa gtatgctaaa gcccagaagg
     1321 ccaagaatgg taagtttgga aatggtatgt ataaaatcgg attagaaatt cctgctggag
     1381 aatatattat aatgtcaaat tcttctgatg catattatga agttagaaat gatagcttag
     1441 gtaatgcaga gggaatagtt actaatgata ctttttcagg aagaagatat ataacggttg
     1501 aggaaggtca atatttaata ttaaatgatt gttatttaat tgaaaatgaa tagatgtaat
     1561 tgtattt
//
LOCUS       DAEORZ010000297         1558 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEORZ010000297 DAEORZ010000000
VERSION     DAEORZ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.303"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(470..1432)
                     /locus_tag="KRH39_003847"
     CDS             complement(470..1432)
                     /locus_tag="KRH39_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8513708.1"
                     /translation="MISTNVINKVNDITKDSVTNNRVYGADRHETNANVIKKFYTDDE
                     LEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVYKIGG
                     GLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIREKDY
                     TLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNASDTTG
                     NGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLYVLRN
                     NDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT          494 a          261 c          178 g          625 t
ORIGIN      
        1 tttttaatct tttaactttt gaaaatatgt caaagtgtat ctaacccaaa atataaataa
       61 tatataagta atatataaaa ataaacttat aaaactcaaa aaataaaaaa attaaatata
      121 tttaatacat ttaattatct atactatatc atttattgac ataaaaatcc acaaaaaaca
      181 cacaaacaca caataaaata tagtaattaa gcttttattg tgtattacaa atgaatactc
      241 caactcttga caattaactg tagccttaag atatgtaaat tcttcatata atataaatcc
      301 ttttacaatt aatcataaaa agactgtata tacctcattt cgcacttata tgcacaatgt
      361 atatacagtc tttattacta ttataattac attcttatct tatagcttta gtttaaattt
      421 aaaattgcag attaatttat tttaaatttt tctatgctct cattgtagac tattttaatg
      481 aatcttctat gcctttacct atttgtgtac ccatttcttt tactttattt gatgtattaa
      541 gtaaactcat atctttctca ttatccaaaa atgcacattc tacaagtaca gctggcatat
      601 cattatttct taaaacatat aaataatctt ttgtagggtc agttgatagt gttcttgttt
      661 ttgcacctct attttttaga ttaaatttct ctaagattct atttaatata tttgtagcta
      721 cagttttagt agtccctcca tctttatctt taagcttata aaatacttct acaccatttc
      781 cagttgtatc agatgcatta tagtgtatac tcgtaaataa atctggtctc aaagagttag
      841 ataaagcagt tctatttcca agagataaag tcttatctgt gtctcttgtc attattacat
      901 tgaatccctt tgaacgtaaa tattcagttg tcgcaagtga agtatttaat gtatagtctt
      961 tttctctaat accccctaat ggtttacctg ttgttccagt gtccgaacca ccatgtcctg
     1021 ggtcaatcat aactgtcttg cccccactat ttcctggttc tgttggagtt gtattgtgtt
     1081 tagataatga tgatgctata gagctcatta ccttagaagt caatcctcca cctattttat
     1141 ataccttatt ggccgattta gcttctaagt tttctttatg gtatgcagat acatatgtct
     1201 ttggtgttat aagtattgga gattttaagt tggcagccaa tggacctgct gctaaagcat
     1261 caacaagtac atctgattta gctactaaaa cagcttctaa ctcatcatct gtatagaatt
     1321 tttttattac atttgcattt gtttcatgtc tatctgctcc atagactcta ttattagtaa
     1381 cactatcctt agttatatca ttcaccttat ttataacatt tgttgatatc atttgaggtc
     1441 cacctataaa ataagcattt tgtaaatcct cactctttaa ccatttatat gtattgtctg
     1501 taatactatc tttatcagtt aatataattg gttgcttgtc ttgacctgct tttgctgc
//
LOCUS       DAEORZ010000298         1538 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEORZ010000298 DAEORZ010000000
VERSION     DAEORZ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.304"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            1023..1412
                     /locus_tag="KRH39_003848"
     CDS             1023..1412
                     /locus_tag="KRH39_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8513709.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT          678 a           93 c          269 g          498 t
ORIGIN      
        1 attaatgaaa aaatttaaac tttgtaatat ataaataaaa aaactctaaa atagaaggat
       61 ataattatat agaatgtttc aattaaacaa gtcaaaaatt ataatcttaa aattaacatt
      121 attcttaaga agtaagaaat ttaagaagta aaaagtttag tgataagtgg taataagcaa
      181 gaaatttaga aagtaggaag tttagtgata agtggtagta agtaagaaat ttaggaagtg
      241 ggaagtttag ttataagtag tagtaagtaa gaaatttagg aagtgggaag tctattagta
      301 ataatttata gcaagtaaga aatttaggaa gtaggaagtt tagtaataat ttatagtaag
      361 taagaaattt agaaagtagg aagtttagta ataatttata gcaagtaaga aatttagaaa
      421 gtaggaagtt tattagtaat aatttatagc aagtaagaaa tttagtaata atttgtagca
      481 agtaagaaat ttagggagta ggaagtttag taataatttg tagcaagtaa gaaatttaga
      541 aagtaggaag tttagtagta atttatagta agtaagaaat ttagtaagtg agaagtttag
      601 ttataattta tagtaagtaa gaaatttagt aagtgagaag tacagtaata agttgtagta
      661 aataaaaagt ttaggaagta ggaagtacag taataagttg tagtaaataa aaaatttagg
      721 aagtaggatt tactgaaata caaatattta aaatcaaaga gtttaaaaga aaggagagct
      781 actggacaag tatccaaaat agatagctga gtacaaaata tttcataaaa ttaagcaata
      841 taatacaaga gaattttgac attatttata tatatttacc gtgtacaaat agctaaattt
      901 aggattaaga ttgaaaaata tcttataagt ctgagcttat ataaatccgt ataagatgta
      961 tttaatcgaa aaattttatt atggatattg cttgtctata ttctgagtag caaggtttta
     1021 tcatgactat aaaaaagttg ccacaatcag aattaaagat aatgaaattt atatggaaat
     1081 cagattccaa agtaacatct agagatattg ttttaggaat ggagcaaaaa tatcaatgga
     1141 aacaaacgac aactttgact cttttatcta gattagtagt aaaaagattt ctaaattctc
     1201 aaaagataga taaatataca cattatgaag ttttaattaa agaaaaggaa tacataggag
     1261 ttgagacaag agattttttt agaaatattc atgattcttc tataaaaagt ttattgttgt
     1321 cattacatga aaatattaat ttgagcaaag atgatatctt acttattgaa gagtggataa
     1381 aaaatctaaa agaagaggag aaagacgtat aagcaaacgt atttcttata gcatcaatat
     1441 aaagtgtatg taagtgtatt tatgtaatat attagtctac ttaagtaata tgacaaatga
     1501 ttataagtgt attatgatac tataatgtga atctaaat
//
LOCUS       DAEORZ010000299         1526 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEORZ010000299 DAEORZ010000000
VERSION     DAEORZ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.305"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            414..791
                     /locus_tag="KRH39_003849"
     CDS             414..791
                     /locus_tag="KRH39_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8513710.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     NDENIGKDKLDFLESYFDNLKDE"
     gene            784..>1526
                     /locus_tag="KRH39_003850"
     CDS             784..>1526
                     /locus_tag="KRH39_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBG8513711.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENAVGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLIDCSLKNKKEYA"
BASE COUNT          627 a          129 c          222 g          548 t
ORIGIN      
        1 aattataaaa gtcttattca ataaaattta taattaattt agatatattt gtaaataatc
       61 agtataaaat aaatgtttta ataatacaaa gtcataacta ggatgttatt aaaatttatt
      121 agatattact atagtttatt tttaaaaaaa gtattataat attccttgtg tgaaaatata
      181 ggaattttta ttaaacagtt ttaatgtgta aataacattc tacactattg taatcagata
      241 tgtaaaaagt aaaatattta aatagatata ttttaaaatc taacttttaa tattgtaaaa
      301 aaattagaaa attaacaaaa aatacaaaag ttaacatttt tttcataaag tgtttgacta
      361 aaaaattcac atgttgtaga attaaggtag caggagtttt gagggaggtg taaataatga
      421 taagaaaaat accacaagca gaactaaaag taatgaaatt catttggaaa gtagatgtta
      481 cagtgacatc aaaagatgtt attgaagcta tggaacaaaa atatggctgg aaacaaacta
      541 caacactgac acttttatct aggctggtaa aaagaggatt cttagatgct caaaaaatag
      601 acagatatac acattacaca ataagagtaa aacacaaaga ttatttaagt tttgagacaa
      661 aggatttcct aagtaatata catgatgact ctttaagaag cttaatttca gcattacata
      721 atgatgaaaa tataggtaaa gataagctag attttctaga aagttatttt gataatttaa
      781 aggatgaata attatatgct atatgttttt gaaaatctta taaagacaac tattgtatgt
      841 agtcttggta tttgtctatt attatttttg aaaagatatt tatttaagaa gtttagtaag
      901 agattcaatt attatatttg gttgataata gtctttagaa tgttactttt tctattcaat
      961 tatacaatag tgtatgaagt aaaagaaccc aaagaaaatg cagtaggaaa taatattaca
     1021 caaattgata tttctacaga taataatctt atgctgtatg tagcttattt atggctattt
     1081 gtaactatag tcattgcagt ttatacattt ataaagtata caagatttaa aaatctagtt
     1141 gttgatgtat cttatgatat tgaagataat gatataaact gtatctacaa aaacttgtta
     1201 aaagaattaa atataaaaaa gaaaattgaa ttaagaggtt ctgatgaatt gataagtcca
     1261 gcaggcatgg gattatttaa atcttacata ttcttaccag actatcctta tagtaaagat
     1321 gaactaactt ggattttgaa acatgaatta atgcatttta aaaataaaga tattttaata
     1381 aagtttttag ttttgtctgt tagaataata tattggttta atccacttgt atatgtaatg
     1441 agcaataaag taaatctaga ttgtgaatta tgctgtgatg aaagtgtttt gattgattgt
     1501 tctttaaaga ataagaagga gtatgc
//
LOCUS       DAEORZ010000300         1524 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEORZ010000300 DAEORZ010000000
VERSION     DAEORZ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.306"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            67..222
                     /locus_tag="KRH39_003851"
     CDS             67..222
                     /locus_tag="KRH39_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513712.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            352..1365
                     /gene="aroF"
                     /locus_tag="KRH39_003852"
     CDS             352..1365
                     /gene="aroF"
                     /locus_tag="KRH39_003852"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG8513713.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1387..>1524
                     /locus_tag="KRH39_003853"
     CDS             1387..>1524
                     /locus_tag="KRH39_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBG8513714.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     EN"
BASE COUNT          615 a          150 c          286 g          473 t
ORIGIN      
        1 tatattttct ttgttataat tattaaacaa gaagaataat aaattataaa aatggaggat
       61 aatactatga aaggaatatt aaaagaacta acagcaaaac tttatagctt tttttacttt
      121 atgttctggg gcttttattt tagcgctggc tatttctttt acaaaaatat atgtacttcc
      181 tttctggtga ggccttattt tagtgatttt gagcataagt aaattttata aaaatgatta
      241 ttttatatat ttatataaga tttagtgctt gatagattat tttagagaac tcattagtga
      301 gttctctttt ttattttaaa tataaaatta taatattggg aggtagtaaa aatgatagta
      361 gtattaaaaa tgggagcaga taaaaatgaa gttaaaaaat taattgaggc tattgggaga
      421 gaaggagtag aagttaatcc catagatgga acagaattaa ctgttttagg tttagttgga
      481 gatactagta aaatagatgc caaaagaata gaagcaaata aggtagttga aaaagttatg
      541 catgtggtag agccttttaa aaaggccaat agaaaatttc atccagaacc ttcaatcata
      601 aatgttaatg gaatggaaat tggttcaaag aaaattgcta tgatagctgg gccttgttct
      661 gttgaaacag aagaccaaat agtatctata gctaaagatg tgaaaaaatc aggtgcagga
      721 tttttgagag gtggagcatt caaaccaaga acatcaccat acgctttcca aggattgaaa
      781 tatgatggat tggatttatt aaaaaaagca aaagaaaaaa cagggcttcc tatagttaca
      841 gaaataatgt caacacaaga tatagatata tttgaagaaa atgtagatgt aattcaagtt
      901 ggagctagaa acatgcaaaa ctttgattta ttaaaagaat taggtaaaac aaataaaaca
      961 atacttttaa aaagaggatt atctgctaca attgaagagt ggctaatgtc agcagagtat
     1021 ataatggctg gaggaaatga aaatgtagtt ctttgtgaaa gaggtataag aacttttgaa
     1081 acatatacta gaaatacttt ggatttaagt gcaattttag ctgtgaagaa acttagtcat
     1141 ttaccagtta tagttgaccc aagtcatgca gcaggtaaat catggatggt tgactcttta
     1201 tctaaggcag caattgcagt tggagcagat ggtctaataa tagaagttca taatgaccca
     1261 gcacatgcac tttgtgatgg aaaacaatcg attaagccaa atgaatatga tgaattgata
     1321 agtgaattaa agactatcgc atcagcagtt ggtagagaaa tataatctat aaaaggatgg
     1381 ttaaacgtgg aagaaaaact attagtaaat ttaaaagaaa atagctacaa tatatttatt
     1441 aaaaaaaata tactaaagaa tataggagaa gaaataaaaa aaatatattt tggaaataag
     1501 atttttattg taactgatga aaat
//
LOCUS       DAEORZ010000301         1506 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEORZ010000301 DAEORZ010000000
VERSION     DAEORZ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.307"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            30..641
                     /locus_tag="KRH39_003854"
     CDS             30..641
                     /locus_tag="KRH39_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBG8513715.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            939..1277
                     /locus_tag="KRH39_003855"
     CDS             939..1277
                     /locus_tag="KRH39_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG8513716.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
BASE COUNT          596 a          168 c          198 g          544 t
ORIGIN      
        1 ttttaataat attattttag gaggtaaaaa tgataaacga tattaaaaat aaattcaaaa
       61 attacaaacc atatataaat ggatacaaga atatgaagag agcatctgtt ttaattccaa
      121 tagtagaaat aaataacaca cactatatat tatttgaagt tcgctcaaaa aatttgaagc
      181 atcaacctag cgaaatttct tttccaggtg gtaaaataga atcaggtgag actccatatg
      241 aagctgttat tagagaaact tgtgaggaac ttggaacttt tagtgacaat atagaagtaa
      301 tatcaagtct tgacctcctt ataacaccag taaagtttat catacatcca tacttaggat
      361 atataaaaaa tattaataat ttaaatataa acaaggatga agttgaccat acttttttgg
      421 ttcctataaa atatctttta gagaatcctc caaatactta tagtaatact gtaaaagttt
      481 ttccagacga aaatctacca tatggttcaa ttccaaacca aaaagattat aaatttgaaa
      541 gtggaagtta tcctattctg ttttataaat ataaagatta tgtaatttgg ggaatgacag
      601 caaaaatact agaaaacttt ttaaattttt tatcagaata acactcataa taaattatca
      661 aaacttttac ataagaaatt tataaaagtt tctaaattta acaattctaa aatatcaata
      721 actatatttt gtagcacttt tttattttat tattgtttga atatttgatg gttttcaatg
      781 caatttcaac ataaatacta tgtattagtg tatactgttg atgaattaaa gtatgataat
      841 ctatttaata aagtatgaag taaatgattt taatgtgcct ttatggtaaa attactaata
      901 agtaataaaa ttaattaaaa tatatggagg tgcttataat gtttgctgta ggatttgttg
      961 ttataattgc acttgccttt ttcattttaa gtgtactatt atttaatgga aaagccagta
     1021 tgctactagc aggttataac tctatgagta agaaagaaaa agcacgatac gataaaaaaa
     1081 aattatgtag aagttctagc attgttactt ttattgtctc tataatgcta tttattatgg
     1141 cgtttttggg atatagaata gagagtggtc aaatggacga aaaaaaaatg gttccctttt
     1201 ctataatatt tataatagtc atactttcca gtgtattttt taatatcctc tactctaaca
     1261 aaaaatgtaa aaaataaata gttgtaagtt gagcagttaa ttataaaaga ttgactgctt
     1321 tttcatgtat attgactact cttattaaat attttaacat ttaaaaatcc atgtaaaaat
     1381 aatgaatata gtacaaactt ctaatgatga ttttaccttt gtggttacag tccactatat
     1441 ataatttaat atgataacat ggaattacta atgacaacta agtaagcttt tataaaatat
     1501 aataaa
//
LOCUS       DAEORZ010000302         1498 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEORZ010000302 DAEORZ010000000
VERSION     DAEORZ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.308"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            143..1483
                     /locus_tag="KRH39_003856"
     CDS             143..1483
                     /locus_tag="KRH39_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG8513717.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFAYIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKINKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFIVKIAIPLRNVKKAVNHVI"
BASE COUNT          580 a          120 c          241 g          557 t
ORIGIN      
        1 gatataatta aaaaagaaaa gaaatatgga cttatttgga ataattttga caatctattt
       61 actacacgtg tagagtcgta tataattaaa ttatacataa aatataaaaa aataatttag
      121 taatacgtta ggagaaagta ttatgataaa gtcaagctta ttttggaata ttttcattgc
      181 gatatccttt atgatagaat ggtttctttg taagaaaatg ctagattata caagtgagaa
      241 aaggatttct aacaaaaaaa taaacttttg tatggtaggt attattgttt ttgcttacat
      301 aatatatttt tttgatgtac ctccaaatat aagagtaatt atatctatga taattactat
      361 tgcattttat aaaatctcat atgatgtggg gtttataaag tctgtattaa tatccttaat
      421 ttattggatg ttactaatcg ccacagatgc tttgagtata tctttaacag tttggcttaa
      481 ttctataaat gacatgagta gtatggggac gggagatata tacagaattg agtctataat
      541 attgggaaag atacttttga ttgtgtcttt gcttttatat agagccttaa agataaaact
      601 taatatagaa ataaataaaa ggacattatt ttatatagtt attcctatag tcgctaatat
      661 agcatctttt tttgtaattt ttgaatacat atttaatcca gggtctggta gcttaataca
      721 taatttccaa ttcgttatga tttctgttgt attgcttata tcaagtatca ctttagtttt
      781 aattgctaaa aaattacagc aatataatac tttagttgta gaaaacaata tgattaaaaa
      841 tactttgaag tatgaaaatg agtattatga agatatagaa aagaaatatt taaaaactag
      901 attactaagt catgatatga aaaatcacat cttgtgtata aatgctatga ttaaaaaagg
      961 tattgatgtt acttcttatt taaataattt gcaggtagaa attcagagta atgacttgtt
     1021 atttaatact ggtaatttta tattagatgc tatattaagt gaaaagaaag aagtttgcga
     1081 taagaatgat atagaactaa aaataggaat aaattttagt tgttgtgaag ctgttgaatt
     1141 agtggatata tgtcatatat tttcaaatat tttagacaat gcaattgagg catgtaataa
     1201 aattgatagt ggaaatagag ttatttgtgt taaaggtgat gttatgcaaa atttttattt
     1261 aataagagtt gaaaatacta agataaataa gattattact aaaaatggag atattttgac
     1321 agataaaaaa ggttctcttc atggacttgg aataaaaagt gtaaaaaact ctgtaaataa
     1381 gtatggtggt aatgttgtaa ttgaatatga tgataaaaaa tttatagtta aaattgctat
     1441 tccacttaga aatgtgaaga aagcagttaa tcatgtgatt taaatgtata taaatttg
//
LOCUS       DAEORZ010000303         1497 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEORZ010000303 DAEORZ010000000
VERSION     DAEORZ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.309"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            137..1408
                     /gene="serS"
                     /locus_tag="KRH39_003857"
     CDS             137..1408
                     /gene="serS"
                     /locus_tag="KRH39_003857"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG8513718.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
BASE COUNT          622 a          158 c          281 g          436 t
ORIGIN      
        1 tgagtaaaat ataaataaaa taaaaataac aggttcggat aaagaaaaag gtttcttaac
       61 ctcaagcata aagtatatta tctaagttat ttgagattaa gagataaatc caatcttagg
      121 tactgaggag gaaaaaatgc ttgatataaa aagaataaga gaaaatttag attgtataaa
      181 aaaagcaatg gaaagaaggg gcgaaaaaga ctttaacctt gatgaagtag ttaagttaga
      241 tgatgaaaga agaaagattc ttcaagaagt tgaagttatg aaaaatgaat taaatacagc
      301 atctaaaaat ataccaaatc ttataaaaga aggtaaagat gtagaaaatg aaaagattaa
      361 actaaaagag ttatctgata aaattaaggt aatagaccaa aatcttaaag aagtagaaga
      421 taagatggaa tacctattaa tgagaatacc aaatgtacca catccagaag taccacaagg
      481 tgaaactgat gaagataatg ttgaagtaag aacttgggga aagactacta cctttgattt
      541 tgaatctaag gctcattggg aaattggaac agaattaggt atacttgatt ttgaaacagc
      601 tagtaaaata actggttcaa gatttactct ttacaaagga ttaggtgcta gattagagag
      661 agcattatta aatttttact tagacacgaa tacaaaagta aatggatata cagaagttat
      721 tccaccattt atggcaaata gaaatagttt tttaggtaca ggacagttac ctaagtttga
      781 agaagatatg tttaaaatag aaggtctgga ctattttatg attccaacat cagaagttcc
      841 tttaactaat atacatgcta atgaaatact taaatttgaa caattaccaa taaattatac
      901 agcttatact ccatgtttta ggtcagaagc tggttctgct ggaagagata caagaggttt
      961 agtaagacag catcagttta acaaagtaga aatggttaaa atagttgcac cagaagaatc
     1021 ttacaatgag ttagaaaagc ttactaacaa tgcggaaact atgctacaat tattgaatct
     1081 gccttataga gtagtaaaga tatgtacagg tgatttagga tttacggctt cttttaaata
     1141 tgatgtagaa gtttggatgc caagttacaa taggtatgtt gagatttcat cttgttcaaa
     1201 ctgcgaagat ttccaagcta gaagagcagg aataagattt aaaagagata aagattctaa
     1261 agctgaatat gtacacacat taaatggctc tgggttagct attggaagaa gtgttgcagc
     1321 tatattagaa aattatcaac aagaagatgg ttctgtggtt gttcctgaag ttttaagacc
     1381 atatatggga gtttctgtta ttaaataaga gttaaattat gtatttttaa gcaatacaat
     1441 aatatagagg taataaaaaa taattaatat aaaagtgata caataatata gaagtaa
//
LOCUS       DAEORZ010000304         1490 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEORZ010000304 DAEORZ010000000
VERSION     DAEORZ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.310"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            24..1304
                     /locus_tag="KRH39_003858"
     CDS             24..1304
                     /locus_tag="KRH39_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBG8513719.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDTLKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
BASE COUNT          628 a          194 c          170 g          498 t
ORIGIN      
        1 attaaagata aaaggagtat tttatgaaca tacttggatt aatagttgaa tataacccat
       61 ttcataatgg acacatatat catttatcta aatctataga aaaaacaaat gcaacacata
      121 ctgtagcaat aatgagtgga aattttctac aaagaggcga accagcttta tttgataaat
      181 acactagagc aaaaattgct gtcgaaaatg gtgtagactt agttatagaa ttaccaactt
      241 tatttgcttg tcaaagtgct gaaatttttt ctcatggtgc tataacaatt ttaaattcac
      301 taaactgcat aaattctata tgttttggta gtgaagaagg aaatacagaa attctttata
      361 atatagctga aattttagca aatgaaccaa atgaatttaa gttactttta aaaaaatatt
      421 tagatgatgg tctgttattt ccaacagcta gagctaatgc actttttgat tatatacatc
      481 aagacaaaaa aaataatttt atacaaaatt attcaaagga acaattatca aaaacattaa
      541 attcatcaaa caacatactg ggtatagaat acataaaaaa cctcatccat ttaaacagta
      601 gtataaaacc atttactatc tccagggtcc aatcagaata taattccgaa gaaattgaga
      661 gtgatatttg ttctgctact gctataagaa cttcacttaa agaaaataca gacctttttc
      721 atttaaaaaa tgttattcca gaaaaaactt ataacatcct aaatgaaaaa atagataatg
      781 gttttttacc tatgtttgat gacttcttct ttgatacttt aaaaacaatt atacttagag
      841 atatggacac tttaaaagag tattttgaag taaatgaagg catagaaaac agaatctata
      901 aagatatctt tacatctgaa tttctacatg aactacaatt agcaattaaa tccaagcgat
      961 acactttaac caaaatcaaa cgaactctaa acaatatatt gttaggtatt aaaaaaagcg
     1021 acatgcaact tataaaaaac atagatacta taccttatat aagaatactt gcttttaatg
     1081 ataaaggtag agaaattcta aaagagataa aaaataaatc tgaaataaaa ataataaaca
     1141 agttttcaaa tatatcattt tcattagatg atacgatttt taaaacactc ataagttatg
     1201 atataaaagc tacaaatata tataattcaa tatattataa aaacaataaa gatttagtaa
     1261 aaggccctat ggatttttat actcatccaa tatatgtaaa gtagtttttt aaaataacta
     1321 tatttataaa aatcatttta ttaactcttt attatataag atacaatagt cttactttat
     1381 ttatgtacac atgcttttag gatgacaaca tcaacttaaa tattatgtta ctttctaaat
     1441 ttaagtaaat attgccatcc taaaaatttt acaatttagc ttcagattta
//
LOCUS       DAEORZ010000305         1486 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEORZ010000305 DAEORZ010000000
VERSION     DAEORZ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.311"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..618)
                     /locus_tag="KRH39_003859"
     CDS             complement(<1..618)
                     /locus_tag="KRH39_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8513720.1"
                     /translation="MLDSVYSLAIINMVIFIALFFMLMNKIGYIYRCKTNIQLILILI
                     LSSILIINGMPFGIVPLHALIRIILILSLICKITYQINIKKATINSVIYSLLYILIGC
                     SMYWILQFSWKYVGHYILKANTVINFIIIFIGVYFFDILQKIYNNKKYYLYIILTIAV
                     NVVIVLFVNISTEKMDDLYTIVTKNHIEYSYNVYTMYFSLFMKNTF"
     gene            complement(612..1322)
                     /locus_tag="KRH39_003860"
     CDS             complement(612..1322)
                     /locus_tag="KRH39_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8513721.1"
                     /translation="MIKIAICEDEKEQQELLKRYINQIFDALSVKCRLEVFNSGEELL
                     EGYSKDTDILLLDIQMGQINGIDTARKVRVLDDKVEIIFITALIEYALEGYEVRAYRY
                     LIKPVKYNNLKDNIINCIKEVDIKNKYIMVKAQGSQTKLNVNEITYIEVQKETITIHT
                     LNEVYKINGTMSNIEKEIDCSRFFRCHKSFLVNLEHVKSIKQYVAILENSKEVPVSRY
                     RFKEIKDKFFDLIEEKLC"
BASE COUNT          535 a          233 c          104 g          614 t
ORIGIN      
        1 aaatgtattt ttcataaaaa gtgaaaaata cattgtatat acattataac tatattcaat
       61 atgattcttt gttactattg tatataaatc atccattttt tcagttgata tatttacaaa
      121 taaaactata actacattaa ctgctattgt taaaattata tataaataat acttcttatt
      181 attataaatt ttctgtagta tatcaaaaaa ataaacacct ataaaaataa taataaaatt
      241 tattacagta tttgctttaa gtatgtaatg acctacatac ttccatgaga actgtaaaat
      301 ccaatacata ctacacccta tcaaaatata taacaagcta tatataacag aattaatagt
      361 agcttttttt atgttaatct gataagtaat tttacaaatt aaagataata ttaaaattat
      421 tcttattaaa gcatgtaatg gcacaatccc aaaaggcata ccatttataa ttaagattga
      481 agataatatt aaaattaata taagttgaat attagttttg catctgtaaa tataaccaat
      541 tttattcatc aacataaaaa ataaagctat aaatattacc atatttataa tagctaagct
      601 atacacacta tctaacataa tttctcctct attaaatcaa aaaatttatc ttttatctcc
      661 ttaaacctat acctactaac aggtacttcc ttgctgtttt caagtatagc aacatactgt
      721 tttatacttt tcacatgctc taaattaacc aaaaaactct tatgacatct aaaaaatcta
      781 ctacaatcta tttccttttc aatattactc attgtaccat ttattttata aacttcattt
      841 aaagtgtgta tagttatagt ttctttttgc acttctatat atgttatttc atttacattt
      901 aatttagttt gactcccttg tgcttttacc ataatatatt tattctttat atccacttct
      961 tttatacaat ttattatatt atcttttaaa ttattatact ttactggttt tattaagtat
     1021 ctataagccc ttacctcata accttctagt gcatattcta ttaatgctgt aataaatata
     1081 atttctactt tgtcatccag taccctaact tttctcgcag tatcgattcc attaatttgt
     1141 cccatttgta tatctaataa aagtatatca gtatcttttg aatatccttc aagtaattct
     1201 tcccctgaat taaatacttc caatctacat ttaacagaca atgcatcaaa tatttgattt
     1261 atatatcttt tcaacaactc ttgttgttct ttttcatctt cacatattgc tatttttatc
     1321 aactaattcc accccataca catttatata cttaaattat tcttaacaaa gctaaaattt
     1381 ttcttaatct tcttattcag ttacgtactt tattcttaaa taataatcat ttatgagtta
     1441 ttatggaata ctcataagtg attattattt tttatgcagt atactc
//
LOCUS       DAEORZ010000306         1463 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEORZ010000306 DAEORZ010000000
VERSION     DAEORZ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.312"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            442..>1463
                     /gene="bclA1"
                     /locus_tag="KRH39_003861"
     CDS             442..>1463
                     /gene="bclA1"
                     /locus_tag="KRH39_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HBG8513722.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRSCNKPYILVTFEDRIIDNCYPKCECPPIRITGPMGPRGATGSTGPTGVTGPTGNTG
                     VTGSIGPTGPTGNTGVTGEIGPTGATGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGVTGEIGPTGVTGPTGVTGSIGPTGATGPTGATGVTGEIGPTGATGPTGNT
                     GVTGATG"
BASE COUNT          597 a          181 c          278 g          407 t
ORIGIN      
        1 atatataaaa tatgtggaaa acacttattt cagtattttt taaacttatt ttatttattc
       61 tatttaacaa aaataaagtt ttttataatt tttacttata aaaaaatatt gttatggtaa
      121 tatattctat ttacaatttt ataactttgg tttacaataa tatagacaat aaatcctaaa
      181 aaacataata agaaaaaagt gcttaatatt ttagaaagac tggtttagtt tacaccagct
      241 aagccagtca aggaataatt taaagtgtaa tatataataa tgaaaaagaa catgctatta
      301 gaaatttcta atgacatgtt ctttttttgt agaaatcagc attattatat aaaaatttaa
      361 tactataaat tttaattgta ttgtaactaa gcatgtattt taaacatagt atgatataga
      421 cccaaaatgg agggattata aatgagaaat attatacttt atttaaatga tgatactttt
      481 atatctaaaa aatatccaga taaaaacttt agtaatttag attattgctt aataggaagt
      541 aaatgttcaa atagttttgt aaaagaaaag ttgattactt tttttaaagt gagaatacca
      601 gatatattaa aagacaaaag tatattaaaa gcagagttat ttattcatat tgattcaaat
      661 aagaatcata tttttaaaga aaaagtagat attgaaatta aaagaataag tgaatattat
      721 aatttacgaa ctataacatg gaatgataga gtgtctatgg aaaatattag gggatattta
      781 ccaattggga taagtgatac atccaactat atttgtttaa atattacggg aactataaaa
      841 gcatgggcaa tgaataaata tcctaattat gggttagctt tatctttaaa ttacccttat
      901 cagattcttg aatttacatc tagtagaagt tgtaacaaac cgtatatact tgtaacattt
      961 gaagatagaa ttatagataa ttgttatcct aaatgtgagt gtcctccaat tagaattaca
     1021 ggtccaatgg gaccaagagg agcgacagga agtacaggac caacgggagt aacaggccca
     1081 accggaaata cgggagtgac aggaagtata ggaccaacag gtccaacagg aaatacagga
     1141 gtgacaggag aaatagggcc aacaggagca acaggaccaa ccggaagtac aggagcgaca
     1201 ggaagtatag ggccaacagg agtaacaggt ccgacaggaa atacgggagt gacaggaagt
     1261 ataggcccaa cgggagcaac aggcccaaca ggagtgacag gagaaatagg accaacggga
     1321 gtaacaggcc caacaggagt gacaggaagt ataggaccaa caggagcaac aggtccaaca
     1381 ggagcgacag gagtgacagg agaaatagga ccaacgggag caacaggtcc aacaggaaat
     1441 acaggagtaa caggagcaac agg
//
LOCUS       DAEORZ010000307         1462 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEORZ010000307 DAEORZ010000000
VERSION     DAEORZ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.313"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(77..1369)
                     /gene="brnQ"
                     /locus_tag="KRH39_003862"
     CDS             complement(77..1369)
                     /gene="brnQ"
                     /locus_tag="KRH39_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG8513723.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT          515 a          277 c          187 g          483 t
ORIGIN      
        1 tatttgtaca aataactcct tttactttta gttaactcat ttatttacaa ttgattatat
       61 tttaattaaa caaaacttag tttgttccaa gtgtattaga ttgactcttt gatggtataa
      121 aattaccaat tattccagct attataactg gcaccaccca gttaaatcct aatgaattaa
      181 gaggtaaaga atttactact ggaacagcta ctccataact agatataact gttagtatac
      241 ttacaagcaa tgacatatat actgctcctt tgtatacatt atcattctta attttttcat
      301 tgaagaaagc taaaattatt aaaacaattg atggtggata aactatactt agtataggag
      361 atgctatctt tattatagtt ccaacaccaa aagatgctac tactgcactg aatacagata
      421 cagctaatac tattttttca taactcaatt taccttttgt aagtctagag aaatattgac
      481 ctgtggcaga tgtaagacct atagatgtag ttaaacatgc taatgatact actactgcta
      541 aaatcgcttt cccaacattt ccaagcaatc cttcagttat atttactatt acttgtgatt
      601 gtactgcatc tgttccatac attgttgata cagttgcccc aaggaaacat aatcctccat
      661 aaactagagc taatcctcca caagcaagaa cacccgcctt accaatcatt ttcaacttgt
      721 cactaatagt agaatatcct ttatcattta aagatactat tagcactgat gcaagtgcta
      781 ttgctgcaaa agcatccatt gtctgatatc cttgacctat accttctgca aatacattag
      841 gtatcatagg tttatctact atatctccaa gaggtgatat aatacctttt ataattataa
      901 atgctaaagc tattagaaga gctggagtta aaaactgtcc aactatatct acgaccttag
      961 aaggtcttat tgttagtaca tatgaaattg caaaaaaaag tatacagaat aataacactg
     1021 gaattgaagt tccaaataaa ggcattatac ccatttcata agttgttgca cctgttcttg
     1081 gtatagctaa gaatggtcct atacataaaa tatctgcaca acctataaga attcctaact
     1141 tcataccaca tcttttaaac atttcaacta cgtttccatc gaatttagct gtagccatta
     1201 ctgctagaag tgccaatcca gcatcagcaa acgtaaatcc taagaaggca attaaccatt
     1261 ctggacctgt aattattccc aagtaaggtg gaaatattaa gttacctgct ccaaagaaca
     1321 ttgcaaataa cgcaaatcca aatactataa catccttggt cttacccatt ataaaatccc
     1381 cctttttaac cttattaaat taataaaatg tctcgattgt ttacgcttgc ataagtaagt
     1441 tgaattaaac aataagaact ta
//
LOCUS       DAEORZ010000308         1407 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEORZ010000308 DAEORZ010000000
VERSION     DAEORZ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.315"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            75..443
                     /locus_tag="KRH39_003863"
     CDS             75..443
                     /locus_tag="KRH39_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG8513724.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            446..817
                     /locus_tag="KRH39_003864"
     CDS             446..817
                     /locus_tag="KRH39_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBG8513725.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(953..>1407)
                     /locus_tag="KRH39_003865"
     CDS             complement(953..>1407)
                     /locus_tag="KRH39_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513726.1"
                     /translation="NINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEISY
                     TYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEVT
                     RKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
BASE COUNT          529 a          162 c          214 g          502 t
ORIGIN      
        1 aaataatggt tgaaaaatct aattaagagt actataattt aattaagtaa ttacttaatc
       61 aaaacgaggt acttatggaa aacttggtta aaatatttaa agcattgagt gatgaaacta
      121 gacttaacat acttatatta gtatccaaga gaaatatatg tcaaaagggg atttctaaat
      181 atctagggat ttcagattct gctgtttcac aacatataaa aatattaaaa gatgttggga
      241 taataacagg atataaagaa ggatactatg ttttatatca tattaataaa gaatcattta
      301 atatatgtgt agattttata aattctatgt taagtaattc tagtgaaagc tttatagatg
      361 tatttgatgt gaatacaata catctaggat gcagtaaaga atgtaaatct ataaaaaaat
      421 gttgtaagag aagggagaaa taataatgaa agtatgtata ccagtagaag agaataaagg
      481 attggatagc aaaccatatg gacattttgg ttctgctcca atttttgttg tgtgtgattt
      541 agaaagtgga gaagttaaat ctttagataa tggagattta gatcatgaac atggaaaatg
      601 tcaaccatta aaagcattgt cagggacagc tgtagatgca gtagttgttg gaggaatagg
      661 acaaggagct ataattaaat taaatggaat gggaataaaa gtttacagag cagaaggtga
      721 tactataagt gctaatttag acctacttaa aaatggaaaa ttagtagagt ttccatcaga
      781 ccatacttgc tcacatgatg gatgtggaca tcattaaaaa aaacagctga tgaaatatat
      841 gcttcatcag ctattttttc ttttaataaa tacaatttta ttcttttaaa accaaaattt
      901 aaatttttat gttttttgta gtatccttga taatatccca agtttactac ctttagattt
      961 tttttgtatg tttttctata tagtcaatat catttaatat gtcattaagt tccttattaa
     1021 gtgtttttaa atcatcacca tctacattgt agtttaaaaa tagttcctct acattatcaa
     1081 tgatagaatt gacttttcta gtaacttcaa cgaatgtttt aaagttacta ctatttttag
     1141 catttagaaa tttttttaaa tcctttgata taaatgatac tatttcatat tcatcattaa
     1201 atagacctct accaatagga tatggttcaa ttctctgtaa aaaatattgg tctttagcat
     1261 taatattata agtataagat atttcccaac cgtctcctaa atcaaacttg ataaaatata
     1321 atgtactact tacttttgat attcttgtgg caccaagttg tcttaaaaga acttcaatcg
     1381 aatcaccttg aacagtatta atattca
//
LOCUS       DAEORZ010000309         1356 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEORZ010000309 DAEORZ010000000
VERSION     DAEORZ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.316"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            531..1211
                     /locus_tag="KRH39_003866"
     CDS             531..1211
                     /locus_tag="KRH39_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8513727.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
BASE COUNT          595 a           85 c          199 g          477 t
ORIGIN      
        1 attatttaag aatatttaat ctctaaagct taaattacaa taatattata aaaataaaag
       61 tgatatattg tgtaataatc gaaattttat actttggtta aaataatttt atatatagtg
      121 cctaatgtat aacctaatgc tcgtagacaa taatgtggat attatcaaat ttatttttat
      181 aatagaaatt ttaatttata tctatattaa attgttaaag atataattat gagaataaaa
      241 aatatagctt tgaattatat aattgtatat gcttatcttt atacagtaaa taaaaaatca
      301 aatgaaatac caaataaagt gtatatctta aaataactta ctagagatac aaggatgcta
      361 tgtgaatttt gaagtgtgca gtttagataa taaatatata taaataataa agaaaatgga
      421 tttatagtat ttaaaatttc ttataagggt aaaaaatgct attagaaaat gtactaaaaa
      481 tatttttcca attctttata gaaaaataat gatatttagg agagattgta atgcaaattg
      541 atgtattgat aattgatgga gataagaata gttctcaaga gctgaaaaat tttttagaag
      601 gaaaggggat aaatgtctat ttagcatata attgtaaaga tgctattata aaaatattta
      661 gcaagaaatt tgacttaata tttttagaga taattttagt tgatggtgat ggttgggatt
      721 tttgtaaaaa aattagaaat ataactactt gcccaattat tcatataact tatataaatg
      781 aaaatcaaag tattttaaat gctttaaatt ctggaggaga tgattattta ataaagcctt
      841 taaatttaga aatattatat gcgaaagtga aagctatttt aagaagaaga gatttatttt
      901 taaataataa taaaaataat ataaaactag aagattataa tagagctagt ggagtaataa
      961 agttagaaaa taaaatgata aagttaactc caacagaaaa tagattatta aattacttta
     1021 tagagaatgc tgaaaagact ctaactatta aggagatata tgaacatgta tggatgaatg
     1081 agtatttaga tgataattat agtgtagtgg ttgctgtgag tggattaaga aagaaaatag
     1141 agaaagatta taaaaatcct caaaaaatta taacgataag agaagtaggg tattacttta
     1201 acaagatata aagctttata aacagaatga atgataaggc atagataata tgttagtata
     1261 ttacaagagt ttttgttcta acgatatatt tgtaaaatta gatttaattt acttaattaa
     1321 attatagcag tagaataaat ataaaaaatg tctgaa
//
LOCUS       DAEORZ010000310         1306 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEORZ010000310 DAEORZ010000000
VERSION     DAEORZ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.317"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>1306
                     /locus_tag="KRH39_003867"
     CDS             <1..>1306
                     /locus_tag="KRH39_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513728.1"
                     /translation="NEYLKIIIYLIRNKYYKCDVLSCIKKYYIEDESNLSLIIKTLYE
                     KELVCDTKDQTYIQIIIAILRNIQNNGFYRVISKKSKLDINIWRKFVQQSIKYDQIIY
                     DLPKYHYDLLNDELLNIKHGYINMPTNYSKIEVIKIVIFNYLHTYQDKKIAYIHVDKD
                     DRAINAIKEYFTEYSTRIEFIEIDSINNYSEIATDNNFIIIDDFSNVSNKYYGTRFEI
                     MISLALKLEKRLLVLNNNYLKMDSNQLENINYNEDINRYSNIVNYYFKDGKNKELEIE
                     YEKERFVTESYLLNKEINNKINLKGIKLNSAGDKSLLSAINLISKGYKVSIFIPQKNS
                     AKSGINKICIKLLNLIDRLNGKILYDKNCIDDSNLLDRAELLLGRESIYMKLFRLGVI
                     VIHSDMPQEVIEYMRYFIQCGKYKIIIGDKILTKNLSKYTDV"
BASE COUNT          599 a           97 c          178 g          432 t
ORIGIN      
        1 aaaatgaata tttaaaaata ataatttatt taattagaaa taaatattat aaatgtgatg
       61 tactatcttg tataaaaaaa tattatatag aagatgagag taatttaagt ttaataataa
      121 aaactttgta tgaaaaagag cttgtatgtg atactaaaga tcagacctat atacaaatta
      181 ttattgctat attaagaaat atacaaaata atggattcta tagagttatt tcaaaaaaat
      241 caaaattaga tataaatata tggagaaaat ttgtacaaca aagtataaag tatgatcaaa
      301 tcatatatga tcttcccaaa tatcattatg atttattaaa tgatgaatta ttgaatataa
      361 agcatggata tataaatatg cccactaatt actctaagat agaagtaatt aagatagtaa
      421 tatttaatta tttacataca tatcaagata aaaaaatagc atatatacat gtagacaaag
      481 atgatagggc tataaatgca attaaagaat attttactga atattcaaca agaattgaat
      541 ttattgaaat agattcaatt aataattata gtgagatagc tactgataac aattttataa
      601 tcatagatga ttttagtaat gtttcaaaca aatactatgg tacacgattt gaaataatga
      661 tatcattagc attaaaatta gaaaaaaggc tattagtatt gaataataat tatcttaaaa
      721 tggactctaa tcaacttgaa aatataaatt ataatgaaga tattaataga tatagcaata
      781 tagttaatta ctattttaaa gatggaaaaa ataaagagct tgaaatagaa tatgaaaaag
      841 agagatttgt tacagaaagt tatttgttaa ataaggagat aaataacaaa ataaacttaa
      901 agggtattaa attgaacagt gcaggagata aaagtttact atctgcaata aatttaatat
      961 ctaaaggata caaagtatca atatttatac cacagaaaaa ttctgcaaaa agtggtataa
     1021 ataaaatatg tataaagcta ttaaatttaa ttgataggct taacggtaaa atattgtacg
     1081 ataaaaattg tatagatgat tcaaacttat tagatagagc agagttattg ttaggtagag
     1141 aaagtatata tatgaaatta tttagattag gggtaattgt aattcattct gatatgcctc
     1201 aagaagtaat tgaatatatg agatatttta tacaatgtgg aaaatataaa attatcatag
     1261 gagacaagat tttaactaaa aacttaagta aatacacaga tgtgtt
//
LOCUS       DAEORZ010000311         1269 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEORZ010000311 DAEORZ010000000
VERSION     DAEORZ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.318"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(770..>1269)
                     /locus_tag="KRH39_003868"
     CDS             complement(770..>1269)
                     /locus_tag="KRH39_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8513729.1"
                     /translation="YLREKSKNNIKVYAYKSEISYITGEETPFKLYMLEQMVDKIDDK
                     MLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEVIHTGGHTRGHICLYLKESKVL
                     IAGDLLQVENGELKPVDVMHSNKQELKDAIKNISNYDIETIVFSHGGLYQRNIIETLK
                     NLIIE"
BASE COUNT          403 a          192 c          101 g          573 t
ORIGIN      
        1 tttttcttca ctctgcttat attatgatac ataatttaaa catttttctt cactttgcat
       61 tatgatacat gatttaaata tttttactca ctctacatta tgatatatta tttaaatatt
      121 tttcttcact ctgattatat tatgatacat aatttaaaca tttttcttca ctttgcatta
      181 tgatatatgg tttaaataat cttctttact ttgtattatg atacatgctt taaatatttt
      241 tacgcacttt atattataat acatgcttta aataatctta ttcgctttgt attataacac
      301 attatttaaa tatttttact cactctgcat tatggtacat tatttaaata tttttcttcg
      361 ctttgcatta tgatacatta tttaaatatt tttactcacc ttgtattatg gtacattatc
      421 taaatatttt tcttcactct gtattatgat atataactta aatattttac ctcactctgc
      481 attatgatat atgatttaaa tatttttact cactttgtat actaaagttt atttaaaata
      541 ttttctttcc tattatacta tgctgttaaa tatttttcaa ctgaagtatt gtagaacttt
      601 aaaattacta taaaaaatta tattctaaaa tacttgtaat actacatatc acccttaaca
      661 cttaagtata attttaaagc aaaattctta ttacttatat ttaattttga ttctacatct
      721 ttagatataa ttttaagtaa tttaagaaat tattgtaaat catatagtac tactcaataa
      781 ttaggttttt caatgtttca attatgtttc tttgatataa tccaccatga ctaaaaacta
      841 tcgtttctat atcataatta cttatatttt taatagcatc ttttaactct tgtttgttgc
      901 tgtgcattac atcaacaggc ttaagttctc cattttctac ctgtaacaaa tctccagcaa
      961 taagtacttt agactctttt aaataaagac atatgtgacc tctagtgtga cctcctgtat
     1021 gtataacttc tatttcctca cctagattca atttttcatg gttatctaat gacacatcta
     1081 ctttagtata tgatgattta aatccaagtc ccataacatt tagcatacta agcatcttat
     1141 catcaatttt atctaccatt tgttccaaca tataaagttt aaaaggagtt tcttctcctg
     1201 ttatatagct gatttcactt ttgtatgcat atactttgat attattctta gatttttctc
     1261 ttagataat
//
LOCUS       DAEORZ010000312         1238 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEORZ010000312 DAEORZ010000000
VERSION     DAEORZ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.319"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(342..1211)
                     /locus_tag="KRH39_003869"
     CDS             complement(342..1211)
                     /locus_tag="KRH39_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG8513730.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          472 a          186 c          108 g          472 t
ORIGIN      
        1 gcatatttga cactttacct tttattatac catttattat cataaatata ataattctat
       61 ttttttaaaa taaatattta taataatcta aaataaatat ttataaattc taatcaatcg
      121 taaataataa tctaaaatac atcaaaaaat aaactaaaaa atatcgaaaa ataatttaaa
      181 agacatcaaa actaaatctt ctaatcagca attaaaagtc tattttctta gtttaaaaaa
      241 taaagataga ttttttcaaa acactagata aataggtaaa aaaataaaca gttagtaaaa
      301 taaattttta ctaactgttt atttttattg tttaatatgt cctataaatc ctctaagtaa
      361 tattttcctt gcaaaaattc tgattctaaa cctagatttt taaaataatc gaccatgccc
      421 atacacgcaa atttttctgt agaataatga gtagcaccta taacattaat taaattatct
      481 tttgctattt gatggaactc taatactggt tcaaaatgtt ttaaaggctt agtaaaccca
      541 gtaatatata gatttatacc aagttcagca acttctcttg ctacaaatgg ataacttcct
      601 ccccctgctg caatagcaac taaaccatct tttaatacag aatctccata atcatacaat
      661 ttcacatcat gaccaacaga ttttttaatt aattccttaa tctcttctat tgattttagt
      721 gatgttttac atataactcc tacctcaatt ttatcgtcat atttacaaaa tggtttgact
      781 atttctaaac ccatagcttt tgcaaaacta acagaagttg aatagtcact atagttgtct
      841 agtggagaat gtaatacata aaaagaaatt ctacgatttt tcatctcttc catataagat
      901 agtggtatgt cgtgaaatgg aaaaccttca tctgatgcta tgtatcccat tgcatgatgt
      961 gaaaaaatta aaatatcttt ttcatcttta tctataactt gcttaattat ttctttatca
     1021 ggaaaagtag ttgtgtatac tttattaata ttttgagcat tatctaatac taatcctatg
     1081 tatttttttc taaattctgg agtaataaaa tcattttcaa aattcataaa actccaatca
     1141 tcattaatat ttttaatatt aaattctaaa tctaattttt tataaagttc aattgcctta
     1201 ataataacca ctcctttata ataatattga attactac
//
LOCUS       DAEORZ010000313         1196 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEORZ010000313 DAEORZ010000000
VERSION     DAEORZ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.320"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            111..368
                     /locus_tag="KRH39_003870"
     CDS             111..368
                     /locus_tag="KRH39_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513731.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(478..1158)
                     /locus_tag="KRH39_003871"
     CDS             complement(478..1158)
                     /locus_tag="KRH39_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG8513732.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          496 a          147 c          133 g          420 t
ORIGIN      
        1 tcgtattaaa aaataaaata ctaagtttaa aaatttaaat aatctataaa tcaattataa
       61 aatattattt cttagaaatt aaaatgaatt aagtaaatgg aggaattgat atgataataa
      121 ttagaagtca agataaaact aatttaatgc agataaacca aattaaaata gatggtagtc
      181 aagtttatgc agtatttgaa agtaaaattg atactgtaaa aataggagat tatgaaaata
      241 atacaagagc tatacaagtg ttggacaata tacaaaactt cattgaaaat ggaacaaaat
      301 atgattatat aacatcaaat aaagtcagat acaatgttaa taaaatattt cagatgccag
      361 ctaaatagtg agtgtgcatt ttaatttaat aactaaagcc cactttcttt tgtagtatga
      421 aagagggctt tacatatttg taaatatcga agtacacttg ttttatcaat aaattattta
      481 ataaggtata atggaccaat taaacaataa aaattctcct aaaacagcaa gtattaaata
      541 tataatagct tcatttttgt aaagagaggt aatttttttg cttatgtcat catcaattgt
      601 aatagcaatc tcatcttgaa taatattttt atatatagat gggaaaatat cttttttctt
      661 tataagatta aataaattat ttacacctac ccatataaaa tatcctaaaa cagcacctaa
      721 tgtgttcatc attaaatcat caatatctgt agctcttaag ttaaacaatt gacaaagttc
      781 aattgctaat gaaaaaccta atccagttaa tgaaacattg ataatatttc taaaattttt
      841 ccaaattaaa ggtagtaaaa atcctaaagg cataaacata attatattta aaatatatgt
      901 taagatgcca actgagtcaa aaggtattag atttatttca tcaattctaa aaagcccact
      961 ataccttcct atatcccata tgctaccaat tccagcttta ctaagtgcta gatagatata
     1021 taataaaaaa atatataccc atactaaatg tatcattaca ttcttttcaa ctttcaaatt
     1081 ttttcgtttt ataacgacta tttggtatat taagcatggt aatataacgc aaataaatga
     1141 ataaaatgtc catatcattt tattttgtcc cctttcaaat ttatgttttt agtttt
//
LOCUS       DAEORZ010000314         1132 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEORZ010000314 DAEORZ010000000
VERSION     DAEORZ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.321"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            287..988
                     /locus_tag="KRH39_003872"
     CDS             287..988
                     /locus_tag="KRH39_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBG8513733.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKKYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
BASE COUNT          452 a           99 c          173 g          408 t
ORIGIN      
        1 atgttttata ttaaaatatt ataatattac aacgaattgt tacaaaatta cttattatta
       61 accatgaaca tttgttttgt gctatcatat ttatataagg gaattatcat aaaaaatatc
      121 ttgttcatac agttttatat agaaaaaaga aatgaatatt ttctttatat aagtatattt
      181 tgtaaattgc ctctatattt atagtttaaa taatagtggt attaaaaatc tgacaacaaa
      241 aataaaactt tcaaaaaatt aaatctaaaa agaataggag ataaatatga gtattgaaga
      301 tttatatgat tttgaatgtg ttcctttgaa caatgagatt gatattgatg ttttatttga
      361 aaagatgata gaagatggaa aagaaaaaaa atatacacca attatagtca ttgatgaaaa
      421 agtagggcta ttaagaaaaa atatagatat gattactaaa gagtatggtt ctcttgaaaa
      481 ctatagagag tattgtttaa caaaatataa tgaaattgat gtaaatcaat tttttgattt
      541 taggaagagt gagatttcag aattattaaa ggagctaatt gatttagaag atgattttta
      601 tgattataaa cattttagac ccattatgaa tatagatata tttcatgaat ataacaaagt
      661 ttacatagct aaaattccaa caattaaacc atatgaagta tttgcataca tacctattgg
      721 cggatttaat gattgtccta gagatgaaga acatatagct attgcaaaat attggtatga
      781 aaagcatgga atctttccaa tcgcaattgg atgtgataca gttcaatatt ctgtaaaaaa
      841 tttaactaaa gatggtaaaa aatttgatga cttatgtatt gaattagtat tttattgtga
      901 ggatattatt atacaagggt atgaaacctt aaaggctctt aaggatgtat taaaacgctc
      961 tacaatagtg cttttttggt gggattgata ttctatcatt tagatagtat taaattgtta
     1021 gtcaccaaga atccacttgc tttagttata gggagtgtgt caaactaggt ttttatacac
     1081 agtgtagcat agtaatatgt ttaaattaca aaggttaaaa aaatacttta at
//
LOCUS       DAEORZ010000315         1115 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.322, whole genome shotgun sequence.
ACCESSION   DAEORZ010000315 DAEORZ010000000
VERSION     DAEORZ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.322"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(147..>1115)
                     /locus_tag="KRH39_003873"
     CDS             complement(147..>1115)
                     /locus_tag="KRH39_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HBG8513734.1"
                     /translation="PTGPTGPTGVTGSIGPTGATGPTGATGVTGEIGPTGEIGPTGAT
                     GSTGVTGSIGPTGVTGPTGATGAIGPTGPTGVTGSIGPTGVTGPTGEIGATGPTGNTG
                     VTGEIGPTGPTGATGPTGNTGVTGEIGPTGATGPTGNTGVTGEIGPTGVTGPTGATGS
                     IGPTGVTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIGNSSSITVDANG
                     TFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTTEPGGGGVLSGY
                     FAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT          230 a          265 c          199 g          421 t
ORIGIN      
        1 aaatccataa atttaaaatt tttatgaata tattttatta ttattgttat tttattcatt
       61 ctaaaatagg taaaactcaa tatataaaaa tacttttgtc atttaaataa agcctaaata
      121 gtttttaaga ttattttaga cacgttttaa gtcataacag tatcagctat tctaaatatc
      181 gtcaaagtag ccgcagttcc tgctgattgc ccatttcgta tccctactgt tgtagatgaa
      241 aagttattta ttgtaaaagt agtcccacca aataaaaaac cagcaaaata tccagaaagt
      301 actcctccgc ctcctggctc agtagtaagc cctgccaaat cttttcctcc taaaattggg
      361 aataggatag cgaaagaata ttgatttata cttgatgaac ctggttgtaa tgctactgaa
      421 actgatatat aatacacacc attttcttgt actgtaaacg taccatttgc atctactgtt
      481 atacttgaac tatttcctat tattgtttgc caacctgtta tagcatctcc attatttagt
      541 gtttgtggag atggtgcatt tacgaggaag ttagcaactg gctgagagga attgcctgtt
      601 gctcctgttg gacctgttac tcccgttggc cctatacttc ctgtcgctcc tgttggacct
      661 gttactcccg ttggtcctat ttctcctgtc actcctgtat ttcctgttgg acctgttgct
      721 cccgttggtc ctatttctcc tgttactcct gtatttcctg ttgggcctgt tgctcccgtt
      781 ggtcccgttg gtcctatttc tcctgttact cctgtatttc ctgttgggcc tgttgctcct
      841 atttctcctg ttgggcctgt tactcccgtt ggtcctatac ttcctgtcac tcctgttggg
      901 cctgttggac ctattgctcc tgtcgctcct gttgggcctg ttactcccgt tggtcctata
      961 cttcctgtca ctcctgttga acctgttgct cctgttggtc ctatttctcc tgttggccct
     1021 atttctcctg tcactcctgt cgctcctgtt ggtcctgttg ctcccgttgg tcctatactt
     1081 cctgtcactc ctgttgggcc tgttgggcct gttgg
//
LOCUS       DAEORZ010000316         1099 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.323, whole genome shotgun sequence.
ACCESSION   DAEORZ010000316 DAEORZ010000000
VERSION     DAEORZ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.323"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>1099
                     /locus_tag="KRH39_003874"
     CDS             <1..>1099
                     /locus_tag="KRH39_003874"
                     /inference="COORDINATES: protein motif:HMM:NF012632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513735.1"
                     /translation="TCSYQGTFIILIDGSVLSTGYNYFGQLGLGDKVNKKIFTKVNVD
                     NVKKIFSGNTHTFIMKEDNKLLFAGSNNFGQLGCQHFLNNSDNFTTFTEYKNNDYIGF
                     SKSVNLNGDTYLLKDNGEYLLINRNDKIVCGHNFTYILKNDGTIWGAGNNGNGQLGLG
                     DNTHRKVFTKVNIDNVKEIICGNNHVFIIKSDRSVWGTGQNNNGELGLGDNSHRNTFT
                     RLDINNVKKVVCNNCTYVIKNDDTIWVTGYNEHGALGLGDNTHRKVFTKVNIDNVKEI
                     ICGMYHTFIIKNDNTVWACGLNNYGQLGLGDATNRNVFTKVDIDNVKKIACGMYYTFI
                     IKNDNTVWCVGENSRGQLGLGDTTQRNVFTKV"
BASE COUNT          441 a           88 c          206 g          364 t
ORIGIN      
        1 acatgttcat atcaaggtac atttataata ttgattgatg gctcagtgtt atctactggt
       61 tacaattatt ttggacaatt gggactaggt gataaagtaa ataaaaaaat atttactaaa
      121 gtgaatgtag ataatgtaaa aaaaatattc tctggtaata ctcatacttt tataatgaag
      181 gaagataata aactattatt tgctggttct aataattttg ggcaattagg ttgtcaacat
      241 tttcttaata attcagataa ctttactact tttacagagt ataaaaataa tgactacata
      301 ggatttagta aaagtgtaaa tttaaatgga gatacatatt tattaaaaga caatggtgaa
      361 tatttactaa taaatagaaa tgataaaata gtttgtggtc ataattttac gtatatttta
      421 aaaaatgatg ggactatatg gggagcagga aacaatggaa atggtcaatt aggcttagga
      481 gataatacac atagaaaagt gtttactaaa gttaatatag ataatgtaaa agaaattata
      541 tgtggaaata atcatgtatt tataataaaa agtgatagaa gtgtatgggg tacaggtcaa
      601 aataataatg gagaacttgg tttaggagat aattctcata ggaatacatt tactaggctt
      661 gatattaata atgtgaaaaa ggttgtatgt aacaactgta catatgtaat taaaaatgat
      721 gatactattt gggtaactgg ttataatgaa catggagctt taggcttagg agataataca
      781 catagaaaag tgtttactaa agttaatata gataatgtaa aagaaattat atgtggtatg
      841 tatcacacat ttataatcaa aaatgataat acagtatggg catgtggtct aaataattat
      901 ggacagttgg gtttaggtga tgctactaat agaaatgtat ttacaaaggt tgatattgat
      961 aatgttaaaa aaattgcatg tggtatgtat tacacattta taatcaaaaa tgataataca
     1021 gtatggtgtg taggtgaaaa cagcagagga caattaggtt taggtgatac tacacaaaga
     1081 aatgtattca ctaaggttg
//
LOCUS       DAEORZ010000317         1088 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.324, whole genome shotgun sequence.
ACCESSION   DAEORZ010000317 DAEORZ010000000
VERSION     DAEORZ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.324"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            68..1024
                     /locus_tag="KRH39_003875"
     CDS             68..1024
                     /locus_tag="KRH39_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBG8513736.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
BASE COUNT          448 a           89 c          165 g          386 t
ORIGIN      
        1 gtaaaataat tacagtaagg attataaaaa atttttagaa tgtaaattga aatttaggag
       61 tagatgtatg tatatactga atttaaatac aagagagact attgaagatt ttagagataa
      121 attttatgta gcagaaaatt cttatcttat attaagtgct ccaaaaaatt taaaattgct
      181 taaggaaact ttggatatag atgaaataac ttttaatgat tgtcttaaat ttgatgaaat
      241 aaccaaactt gatttatttg ataattacga ttttttaagc ctaaatacat ttgaactaag
      301 agatggagaa gctgttatag aagaggtaaa tatgtattta tcagataatt ttatattggt
      361 tgtagtgaat gaagaacact ttttatttga atttgtaaaa aatattattt tgaagaactc
      421 tcaacttgaa aaaaatccag ttattaatct atttaagata aactacttga ttcttagaga
      481 agttataaaa aatggttttg agtctttgga aaaagttgaa gagttaattc ttcaaataga
      541 agatgaaatg atggacaaca ttaataagaa tcatgtatct agaataagtg atgtaagagg
      601 attaacaaga attatagtta agaatactag acctcttttg tatattgggg atagaatagt
      661 taaggaaaat ataagatatt taaaatattc aaatgtaaaa aaatataatt tagaaaattt
      721 tcaaggtata gattttggaa ttgacaaatt atacaatttc gcattgtcaa cacgagagtt
      781 agcagataaa ttattagata tttattcttc cagagtaggt gagaaaacaa ataacttaat
      841 tacaaaatta accttgttaa cagccatatc agcaccactt acaattataa ctggtatata
      901 tggaatgaac tttagatata tgccagaatt aaactggatt tatggatatc cagcaacttt
      961 attttttatg ctatgtataa tttttgttgg aattataata tttaaaataa aaaaattatt
     1021 gtaaattaca gatgatgttt aattgatttt ataaaaatac atcaattgag aagctgattt
     1081 aagtaaat
//
LOCUS       DAEORZ010000318         1063 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEORZ010000318 DAEORZ010000000
VERSION     DAEORZ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.325"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            20..307
                     /locus_tag="KRH39_003876"
     CDS             20..307
                     /locus_tag="KRH39_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513737.1"
                     /translation="MGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIYGNIDKKKTVK
                     LEVLYRYVTGLEYIAIGLFTKRLDITIIAIILVATITVILYYLVRKRYITI"
BASE COUNT          476 a          106 c          126 g          355 t
ORIGIN      
        1 aaatatcaat cttatactta ttggaataat agttataata ggaactacaa taatatattt
       61 aattaaacct aaaactgctt tttgtagtaa aaaatatttt aataaattag aatcaatata
      121 tggaaatata gataagaaaa aaactgttaa actagaagta ctttatcgct atgtaacagg
      181 tcttgagtat atagcaatag gcttatttac aaaaagatta gatattacaa taatagcaat
      241 aatattagta gctactataa cagtaatatt atattactta gttagaaaga gatatataac
      301 tatataaaat ctaatagaat taatatcaaa agaaggtagc aactaatcac cagtaatgta
      361 aaataatttg tttttcaaag ttaataaaag tcttctttat ggcaatgatt taaatataat
      421 caaatatcgt aacggagatt tttttatgaa taatttaaga aaataattaa tcaaagaaat
      481 tatataagat ggagaactta taaaatctac agatataagt agttttagga gtaaacacag
      541 atgacttatt tcaaagagac aatagctaca agttatcctc attctgtaat acaaaaatgt
      601 ttaatacatt agataagaaa tactttaaat atgtttcata caaggactca aaagaattaa
      661 taagtaattt taaatctgta tataggtcag ttaatgaata agaagcctta tcgactttag
      721 ataaaataga ttaaaaataa tgtaaacagt atacttcaga aattaaaacg tttggaaata
      781 actggaatgt tgtagcatca gcctttagtt ttactaaaat aagtaatatc tataatatat
      841 attacaagta taatcgaagg atttaataaa tatttcaaaa atgtctcaaa aacaaagtcc
      901 agatttacat caaacgcttc actcttaaag atattattct tgacaagcga aaatgtgatg
      961 aaaaaatgga ctatgaaata taaaaattga tataatatat acaatcattt attcatatct
     1021 tttgatagta gaatataatt ttaaaaatta taccctaaaa att
//
LOCUS       DAEORZ010000319         1043 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEORZ010000319 DAEORZ010000000
VERSION     DAEORZ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.326"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..782)
                     /locus_tag="KRH39_003877"
     CDS             complement(<1..782)
                     /locus_tag="KRH39_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG8513738.1"
                     /translation="MRRNTKLLTTGILSMAIVTPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMSLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQDLENMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDVIIIGDENAISKTTANQIKS"
BASE COUNT          335 a          154 c          117 g          437 t
ORIGIN      
        1 gatttaattt gattagcagt agttttagaa atagcattct catctccaat tataattaca
       61 tctttaggat ttaggcgctt taattctgat tttacactat taggcacatt gtttttttca
      121 accaaaagta ttggcgcatt atatgtagtt gccagtggag ttgatataat accatctata
      181 cttacatctc catttattat aactacctta tctgaaccat ttttccagcc ctctttactt
      241 attttaactg ctgtttcgta tctatcgctt cctgtaagtt ctgtaatagg tgcagaagtt
      301 tttctcaagt acttatttac atcttttcta gaagttatgt cttcaattat acattcctgt
      361 gttacattat agtcttttaa atttaatccc atattctcta ggtcttgttt gtttaaagtt
      421 ttaggattat ttttgctgtt tattgttatc ccttgtagtt cccaaacatt taaatattct
      481 gggtctttat tataattgat agttccttca tatgactcag ttgattgatt gtaatttaga
      541 gttgtatcca gtgacatatc atagcattta tactttaatg taatgctatc tgcatctggt
      601 ttttctttaa acttaacact gacttttgag gtactaccta aaactatact ttttttctct
      661 aagtttatat ttaaatcagc gttattttcc atagcattag attcagtagc aaatgccatt
      721 gtaggtgtga cgattgccat tgaaagaatc cctgttgtta ataattttgt atttcttctc
      781 atattaatct ccttacttaa cataaatttc tactttgttt tttaatcttt taacttttga
      841 aaatatatca aagtgttttt acccaaaatt ataaataagt acataaaaaa taaactttaa
      901 aaaaaattat aaaaacaaaa aataaaaaaa ttaaatatat tgaatatatt taattatctt
      961 cactatatca tttattgcca aaaaaatcta taaaagacaa ataaaaacat ataataattg
     1021 taggtttatt aaatttaata caa
//
LOCUS       DAEORZ010000320         1026 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEORZ010000320 DAEORZ010000000
VERSION     DAEORZ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.327"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            223..714
                     /locus_tag="KRH39_003878"
     CDS             223..714
                     /locus_tag="KRH39_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBG8513739.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
BASE COUNT          398 a           97 c          150 g          381 t
ORIGIN      
        1 aagtaatagt tcttatttat agtcaatatt gcctatagaa cttctgattc atttaatttt
       61 taggaaaaaa taataatact tacgctataa acacagaatt ttagttgata atataggtat
      121 ttaaatttaa gatgaaataa tacttttctc aatatatttt attgtaacta agtttaatta
      181 taagttacaa ttaaaaaaac gctacttagg aggaatgtaa ttatggggat gataggatgt
      241 tatactaaaa taagtgaaga gaatgttttt aaattacagc aagcagaaga aaatttacaa
      301 gattttgttt ttgaagatgc caacgaaaat agtactatca atattgataa agcatggcat
      361 gccattcact ttactttgac aggttgtcct tttggtggag atgatgataa tatatttagc
      421 aaattagtac taagtggaaa catacttatg gaaattgatg gagaatttcc tgtcatgttg
      481 attacagcaa atgatgtaaa aaaactatca aaagcaatga acagtttgga agaacaagca
      541 tttagaaaaa gatttaatat taatgagatg ttagaaaata atatttatcc tgttatgaat
      601 gatgagagtg aagatgattt ttttgaatat gtatgggcaa atctaattga gcttaaaaag
      661 tttatacaag aagcatctaa tgatggacta gctgtaatat tttttataat gtgataatta
      721 ttaaaaattg tgaatattag tgtcatcaca tatcttagat atgaagcctt ttgattgtat
      781 agaagaaaat acatattata ttagcctttt aaaatatata agttaaattt aaatctgtag
      841 gtaaatttgt cttatgtctt tataaatatt tatattagca tatataaatt agttttaaat
      901 ttttgcaata tatagttgca gatattttta tttatcttaa aaggctaaat accctatcct
      961 attatatcca ataattcttt tggatactta tcacattttt cattatatat aaaaatagac
     1021 tcacaa
//
LOCUS       DAEORZ010000321         1023 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.328, whole genome shotgun sequence.
ACCESSION   DAEORZ010000321 DAEORZ010000000
VERSION     DAEORZ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.328"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(54..764)
                     /locus_tag="KRH39_003879"
     CDS             complement(54..764)
                     /locus_tag="KRH39_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8513740.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT          342 a          137 c          113 g          431 t
ORIGIN      
        1 gctttaaaaa attaagtttc aaaaataaaa ttctagagat tgaacttcaa aaattaagat
       61 tctaacatat accttgtaaa ttttcctaca acctcttgat attttttacg gcttatagga
      121 attttttcat tctgaacttt ggcataagtt tgagttatac tatctacatg gtttagattg
      181 acaatatacc tttgatgcgt acgtataaaa ttagagggaa gaacagtttc catatcattt
      241 aattttccgt agtattctaa tgttttatca aaggtaacta cagttaattt tcttagctca
      301 cttttaaagt agaggatatc ttttgtatta attttacaaa gattagttcc aatctgaact
      361 gtaataaagt tttctttcca ttgttctaat ttttttagtg cttcttttaa tactttacat
      421 attttttcag ttttataagg ttttaagatg tagttcaaag ctcctacatc atatccatga
      481 aatacatagt cagaaaaacc tgtgacaaag atgattatag tatcttttga atgttttcgt
      541 atagtttttg ctatgtcaac gccattactt tttccaagtt caatatctaa aaaaataata
      601 tctatgtaat aattttcgtg ttctagattg tatactagac tttctccatt tgaatattct
      661 tttatttggt aggggtagcc ccacaaatcc atttctcttg taatgatttt attcagcatg
      721 tttctttgtt gtttttcatc atcacaaata caaatcgaag tcaatttttt atctcctttc
      781 aagttttata tttatatagt taagtatata atatattttg ctacctagat atataaaatg
      841 aaataacaat tatatccaga tagcaaaata aaatttttta tattagtata cttacactta
      901 aaactattct attaaactta ttctgaagtg ctaatgattt tcttattaaa aaatctaata
      961 atctttctta ttataaaata caaatgcttt tattattata aaaagtaatg attttattac
     1021 ttt
//
LOCUS       DAEORZ010000322          997 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEORZ010000322 DAEORZ010000000
VERSION     DAEORZ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.329"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(140..910)
                     /locus_tag="KRH39_003880"
     CDS             complement(140..910)
                     /locus_tag="KRH39_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG8513741.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT          366 a          195 c          115 g          321 t
ORIGIN      
        1 tacaaactat attcaataaa tatataataa agtaaaaaaa aaaaaataac aacatataaa
       61 agaatataaa taatttaaaa aaagcatcaa tatcactgat atcaatgctt taaaaggcaa
      121 tttaattcgt attatttatc taactatttt tgacatagca aagataataa atccctccaa
      181 ctactatgga acctccaacg atatttccaa gtgttacaaa taccaaatta gttataaatt
      241 gaaccatagt tatatttgct cctaatgcca ttcccattgg tataaaaaac atgtttgcaa
      301 cactatgttg gaatccacat aatacaaata gcattattac tgtccaacat gaagccaatt
      361 ttgacattat atcttgagat gctgtagcca tccatacagc taaaacgact agaatattac
      421 aaagtattgc ttttaaaaac attgatgcta tagttgaatt aaccttagct tctgccacag
      481 ctatagcttt tgtagctgca tctcctgtca atgtagaaga ataaaacaca agtgcaacta
      541 gcaaaattga acctactaaa tttgcaaaat aaaccagtcc ccagtttctt aacatttttc
      601 ctaatgttat ttttttgtcc ataagtgcaa tagccattaa ggtgtttcca gtgaataatt
      661 ctgctcctgc cactactaca agcataagac ctactggaaa gaccatagca cttaaaaagc
      721 tagctacccc tgggtctatc ttgccaaaag tttgactaac tatcatactc cccaatgccc
      781 caaaacctat aaatgctcct ccaaataaac ctaatagaat tgcgttttgt gtcgaaatat
      841 ttgctttttt aatgcctgca cttatagttg cttgtgcagt ttccccaggt gtcaagaatc
      901 ctttactcat aaatccttcc cccttaaaaa ttatactttc ccttgtcttt ttatggcaat
      961 aattatataa taacaatatg atatataata aatttta
//
LOCUS       DAEORZ010000323          968 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.330, whole genome shotgun sequence.
ACCESSION   DAEORZ010000323 DAEORZ010000000
VERSION     DAEORZ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.330"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            144..794
                     /locus_tag="KRH39_003881"
     CDS             144..794
                     /locus_tag="KRH39_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8513742.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
BASE COUNT          424 a           92 c          163 g          289 t
ORIGIN      
        1 gcatgattta cagtacaata atttaaattg tagtacaaaa gtactatcaa ataagaattt
       61 ctactttaaa aatattacta aaagtaatta aaatccaata attcaatata atatggcaaa
      121 agaatagaaa taaggtgaga gtagtgaaag taaagtcaaa tttagatgaa attttatatc
      181 ataagattat tgaaagtctt attcgtggtg aatactctgt aggtcaaaaa atattactaa
      241 atgacctttg tgaaaaattt gaagtaagca gaacaccagt tgtacaagca gtaaaaatgc
      301 taaataaaga tggagtgctt actattatga caaatggtaa agtgtacgta ccagagtatg
      361 aatatgatat ggtaaaacaa gtttgtgaaa cgagaacact gattgaaaca tatgctttag
      421 aaaaaatgat gcaagaagag gaagaggttt ttcaacaaaa gctagatgta ataaaaagat
      481 actctgataa atgcgaggag tactatcgtc aagaaaaatc tgtagaactt gcactagcag
      541 atttggaatt acataaagcc attgtagaag gggcaaataa tgagatttta aatgatgtat
      601 atgttggtat acaaggaaga tttatagtag tgaactattt aattcgacca ttaaaaaatc
      661 gtaattatga aggaacagta caagaccatt ttgaaatatt aaaattcatt gaagacagag
      721 atacaaaaaa agcagtagat aaactaagaa atcatattca aggaactatt aaaagattct
      781 gtgaagatga ataataagtt ttgaagttac tatatatact tatctattaa gttgctaaat
      841 gtagtctaag tataatctaa ttatagttta aacataatct aaatataatc taataaatat
      901 aatctaaata tagtgtaaat aagtctaagt atgaattaaa tatagattaa gtatggacta
      961 agtatgga
//
LOCUS       DAEORZ010000324          825 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEORZ010000324 DAEORZ010000000
VERSION     DAEORZ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.331"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            133..309
                     /locus_tag="KRH39_003882"
     CDS             133..309
                     /locus_tag="KRH39_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513743.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT          324 a          108 c           82 g          311 t
ORIGIN      
        1 taaaactggg aatatttaac cttaaggagt taagttatag aaactagtac taaaaaattt
       61 tataaattat gttaattgac taatagaatg cgacttttta tttttactaa attttttagg
      121 gggatggata atatgtttat tggagaaata gacaaatgta cacatatact aactgcttac
      181 atcagtagct catatgatta ttgtaatttt ttagatacac agttagatga ttttatatca
      241 gaatatggag aaacggtagt tgaaacctgc ttacatcaag tcttattatt agtaagtaga
      301 tacaattaaa actaaatttt tctagaaaaa cctttttaga tttatctact ctaataaata
      361 tataattttt tgtactttat aagtaactat aacttctgct aaagtaaaga tgtattggga
      421 gtacatcttt actttcccat tctagaagtt aaaagcatac accataaaat taatcatcca
      481 atacaaatta tatatttcct atgtctaact taatttacaa gaatcattct atatgcatat
      541 tctgcaatcc actaaacatg gctctaaacc tttactattt tatacattct ctataaatca
      601 tacaaatcat atataaaatc cccctaatat gtttaaacca cctataaaat aacaccaata
      661 tttagaatat tcaatatcaa ctgcataact ggtcaataaa aacgtataac tagacaaatt
      721 tcaatattta tataaataat acaagaaaaa caagcatttt atttatataa gttttttatt
      781 ttttgtttat attaaaaaac tatattgatt aatttatttt attta
//
LOCUS       DAEORZ010000325          814 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEORZ010000325 DAEORZ010000000
VERSION     DAEORZ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.332"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..362
                     /locus_tag="KRH39_003883"
     CDS             <1..362
                     /locus_tag="KRH39_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8513744.1"
                     /translation="IEKLLRVELIVMEYLWKKDTLMPKKEIVKEIKQIYGWHKSTIKI
                     LLKRLVNKRYLARYIINFQSYYKIIIDSKEYYTFKKKVLKSSKSIKIMHSLTTIHKNV
                     SKEKLDLLDEYYRNLKE"
     gene            467..814
                     /locus_tag="KRH39_003884"
                     /pseudo
     CDS             467..814
                     /locus_tag="KRH39_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          383 a           63 c          122 g          246 t
ORIGIN      
        1 taatagaaaa attattaaga gtagaattaa tagtaatgga gtatttatgg aaaaaagaca
       61 ctctaatgcc taaaaaagaa attgtaaaag aaataaaaca gatttatgga tggcataaaa
      121 gtacaataaa aatactacta aaaaggctag ttaataaaag gtatttggct agatatatta
      181 taaattttca atcttattat aaaataataa tagatagtaa agaatattat acttttaaga
      241 aaaaggtact aaaatctagt aaaagcataa aaataatgca ttcacttacc actatacata
      301 aaaatgtaag taaagagaag ttagacctat tagatgagta ttatagaaac ttaaaagaat
      361 gatatagtaa aaatcgaatg taattattta ccttaattcc tttgtttgta aaactttatt
      421 gatatagaat ttgacatata cataaattgg taaagggatg tatactatga aaattagtaa
      481 attaccagaa gcggaattaa aagttatgag atatatttgg gaatcagaaa aggtattaat
      541 atcaagagaa atcaccaagt ctatggaaca aaaatatgaa tggaaagata caactatatt
      601 tacagtatta aaaagattac taaagaagag agtttttaga aacggagaaa atagataagt
      661 gtacacacta taatgtatta gtaaaagaaa aataatatca gagattttaa acaaaagaat
      721 ttttaaagaa tacacataaa aattcaatat taagtttaat gtcagcacta tgtagtaagg
      781 atgatgaagt agatggtgat attttaaatt tatt
//
LOCUS       DAEORZ010000326          797 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.333, whole genome shotgun sequence.
ACCESSION   DAEORZ010000326 DAEORZ010000000
VERSION     DAEORZ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.333"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>797
                     /locus_tag="KRH39_003885"
     CDS             <1..>797
                     /locus_tag="KRH39_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021414120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase C80"
                     /protein_id="HBG8513745.1"
                     /translation="TYYYDEDSKLVKGLININNSLFYFDPIEFNLVTGWQTINGKKYY
                     FDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPDGFEYFAPANTQNNNIEGQAIV
                     YQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNPNNAIAAVGLQVIDNNKYYFNP
                     DTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKHFYFDSDCVVKIGVFSTSNGFE
                     YFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNSKAVTGWQTID"
BASE COUNT          292 a           94 c          131 g          280 t
ORIGIN      
        1 aaacttatta ctatgatgaa gatagtaaat tagttaaagg attaatcaat ataaataatt
       61 cattattcta ttttgatcct atagaattta acttagtaac tggatggcaa actatcaatg
      121 gtaaaaaata ttattttgat ataaatactg gagcagcttt aactagttat aaaattatta
      181 atggtaaaca cttttatttt aataatgatg gtgtgatgca gttgggagta tttaaaggac
      241 ctgatggatt tgaatatttt gcacctgcca atactcagaa taataacata gaaggtcagg
      301 ctatagttta tcaaagtaaa ttcttaactt tgaatggcaa aaaatattat tttgataatg
      361 actcaaaagc agtcactgga tggagaatta ttaacaatga gaaatattac tttaatccta
      421 ataatgctat tgctgcagtc ggattgcaag taattgacaa taataagtat tatttcaatc
      481 ctgacactgc tatcatctca aaaggttggc agactgttaa tggtagtaga tactactttg
      541 atactgatac cgctattgcc tttaatggtt ataaaactat tgatggtaaa cacttttatt
      601 ttgatagtga ttgtgtagtg aaaataggtg tgtttagtac ctctaatgga tttgaatatt
      661 ttgcacctgc taatacttat aataataaca tagaaggtca ggctatagtt tatcaaagta
      721 aattcttaac tttgaatggt aaaaaatatt actttgataa taactcaaaa gcagttaccg
      781 gatggcaaac tattgat
//
LOCUS       DAEORZ010000327          779 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.334, whole genome shotgun sequence.
ACCESSION   DAEORZ010000327 DAEORZ010000000
VERSION     DAEORZ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.334"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            171..725
                     /gene="tcdR"
                     /locus_tag="KRH39_003886"
     CDS             171..725
                     /gene="tcdR"
                     /locus_tag="KRH39_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBG8513746.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSIFEEDICFFGYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
BASE COUNT          331 a           51 c          101 g          296 t
ORIGIN      
        1 attagaaaaa aattactaat taattattaa aattaatgta tttataatgc gtattttcat
       61 atgaaattta atttatttgc cgattatata attgtaatga ctgatttaat ttcgatgttg
      121 tcaaaatttt cgaataaatc atcattataa atataagaga ggatgatttt atgcaaaagt
      181 ctttttatga attaattgtt ttagcaagaa ataactcagt agatgatttg caagaaattt
      241 tatttatgtt taagccatta gtaaaaaaac ttagtagagt tttacattat gaagagggag
      301 aaacagattt aataatattt tttattgaat taataaaaaa tattaaatta agtagctttt
      361 cagaaaaaag cgatgctatt atagtcaaat atattcataa atcattactg aataagactt
      421 ttgagttgtc tagaagatat tctaaaatga agtttaattt tgtagaattt gatgaaaata
      481 tcttaaatat gaaaaataat tatcaaagta agtctatttt tgaggaagat atttgttttt
      541 tcggatatat tttgaaagaa ttatctggta ttcaaagaaa agttattttt tataaatatt
      601 taaaaggata ttctgataga gaaatatcag tgaaattaaa aatatctaga caagctgtta
      661 ataaggctaa aaatagagca tttaaaaaaa taaaaaaaga ctatgaaaat tattttaact
      721 tgtaattaat gagtttaaag aaatatttac aatagaaatc aaattttata attaacttt
//
LOCUS       DAEORZ010000328          774 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.335, whole genome shotgun sequence.
ACCESSION   DAEORZ010000328 DAEORZ010000000
VERSION     DAEORZ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.335"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..337
                     /locus_tag="KRH39_003887"
     CDS             <1..337
                     /locus_tag="KRH39_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8513747.1"
                     /translation="KELDINQKELATKVGITEASLSRYENNLREPKSEIIVRLAKALE
                     TSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKEDREAIEKMIDNAY
                     LKRFLKEEN"
     gene            complement(334..474)
                     /locus_tag="KRH39_003888"
     CDS             complement(334..474)
                     /locus_tag="KRH39_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513748.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
BASE COUNT          319 a           90 c           80 g          285 t
ORIGIN      
        1 aaaagaatta gatataaatc aaaaagaact tgctactaaa gtcggtataa ctgaggctag
       61 cttgtcacgt tatgaaaata atttaagaga acctaaaagt gaaataatag ttagattagc
      121 aaaagcatta gaaacatcaa cagattattt attaggcgta aatgataata caaaaataag
      181 caaagaagat aaattaatca tagagaactt atcagtaagt gaaaaaacaa aaaagctctt
      241 agaaaaaata tattctcttg aaaaagaaga tagagaagct atagaaaaaa tgattgataa
      301 tgcctattta aaaagattcc ttaaagaaga gaattaattc tcttcttttt ctttatacaa
      361 aatctcaagt aataattcat aaaaagtaat atcttcactc aataaatgtt ctaatttcat
      421 tcctatatct tcaatttttt ctttttttgt aacagtagtg tttgactttt ccacatatac
      481 catcccccaa caaacttacg ttcttaatgt tagtcagaaa ttcccaacaa atttttaata
      541 aagtttatgt aattgtattt tgattttata aaatatattt tgattatcca aaatttccct
      601 agataattat cttaattata taataatact tttattctac agttgcaata aaaaactcgt
      661 aataataaca aaaaaatcta ttattgtaga atcttgtatg ttttttctat ttttatatat
      721 ttttcaggat gatttttatt ataaaatttt ttataaatat ataatttttt tata
//
LOCUS       DAEORZ010000329          748 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.336, whole genome shotgun sequence.
ACCESSION   DAEORZ010000329 DAEORZ010000000
VERSION     DAEORZ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.336"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            55..714
                     /gene="cprR"
                     /locus_tag="KRH39_003889"
     CDS             55..714
                     /gene="cprR"
                     /locus_tag="KRH39_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBG8513749.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
BASE COUNT          302 a           50 c          154 g          242 t
ORIGIN      
        1 attattaaat atataaatta gaatgctatt agatggataa atggaggagt tattatgtct
       61 aagatattag tagtagatga tgagttagat atgttaaagt tgattgaaaa tgtgttgaaa
      121 agagatggac atgaagtaaa ggtaattagt gatgtagaaa aggctatgaa catggatttt
      181 aattatttta atctaataat attagatgtt atgatgccta agatggatgg ttttgaagta
      241 tgtaaaacta taagaaataa ggttgattgt ccaatactgt ttttgacagc aaaaaatatg
      301 gaaagtgata ttatgtacgg tcttggtata ggtgcagatg actatataac caaacctttt
      361 ggtgtaggag aattaagggc taggataact gctcatttaa ggcgagaaag tagagaaaaa
      421 aagaatttat taactatctc aaatgttcaa tttaactttt taggcaaaga agtttcaatt
      481 gatggtaaaa aaattaattt tactaaaagt gaatatctaa tatgtgaatt tctagcaaaa
      541 aataagggac aagtattttc aaaagaaaga atatatgagt cggtatatgg ttttgatgga
      601 gaaagtgata taagtactat cacagagcat ataaaaaata tacgaagcaa attaaaacaa
      661 tatgatattt atacaataga aactgtatgg gggatagggt ataaatgggt gtaataagta
      721 taagtgttgt ttagtgaaaa tattatat
//
LOCUS       DAEORZ010000330          712 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.337, whole genome shotgun sequence.
ACCESSION   DAEORZ010000330 DAEORZ010000000
VERSION     DAEORZ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.337"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            205..675
                     /locus_tag="KRH39_003890"
     CDS             205..675
                     /locus_tag="KRH39_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG8513750.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT          318 a           53 c          134 g          207 t
ORIGIN      
        1 tagatagaga taaaagtgtt aatggaagat attgaaaaaa taaaagattg atataagata
       61 ctgaagaggt agaaacaaaa gtgttaatag aagatactaa aaaagtaaaa ataaaggatt
      121 aataaaaggt actgaagaga tagagaaaaa aatgttaata gaagatactg aaaaaataga
      181 aataaaaaat taggaggaaa atttatgaaa tatcaggttt cattattagc agtgaaggat
      241 attgaagtat caaaaaaatt ttacaaagaa ttatttgagc aagaaattga attggactta
      301 ggtaaaaatg taacatttaa aggaggattt gctatacaag aagacttcgc atggcttaca
      361 gggattaatc cagatacaat tatacaaaaa tcaaataata tggaacttta ttttgaggtt
      421 gatgattttg acgaatttat tgaaaaactc aattattata aagatgtaga gtttgtccat
      481 aagccactaa aacatgaatg gcaacaaaga gtagtgcgta tttatgaccc agataagcat
      541 attattgaga taggagagtc tatggaagtt atagctagaa gatatttatc tgagggatat
      601 tctgtagaag aaacttctaa aataattcaa catccaattg aatttgtaaa gcagtgtgaa
      661 aatagtaagg aatgattgtt taaattataa ttatttagta gaaattaatt gt
//
LOCUS       DAEORZ010000331          640 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.338, whole genome shotgun sequence.
ACCESSION   DAEORZ010000331 DAEORZ010000000
VERSION     DAEORZ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.338"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(18..>640)
                     /locus_tag="KRH39_003891"
     CDS             complement(18..>640)
                     /locus_tag="KRH39_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513751.1"
                     /translation="CLSSVFIFTQLARVFINKKKINQKIKSRNGFRYDRDFIEARREE
                     IHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKNGFGIYIFSSKEKYEGL
                     WKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDIYKGYFLDNKKNGKGVL
                     YKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNNKLKV"
BASE COUNT          192 a          109 c           34 g          305 t
ORIGIN      
        1 tttttttatt agaataatta aacttttaat ttattgttat ttaatctatt ttgataaaca
       61 ttatttattt tattattatt taaatcttta aaatctaaac ttttacattg ttcatcatct
      121 tcccatattc ctgcttgttt atttccatca tttttatata atacaccttt accatttttc
      181 ttattatcta aaaaatatcc cttataaatg tcattattgg gatatgttaa ttttcccaaa
      241 ccatttttta atccgtattt aaattcacct gtatatattg aaccatctct ataagtatat
      301 tttcctattc catgcatctt gtcatctttc cataatcctt catacttttc cttacttgaa
      361 aaaatatata ttccaaatcc atttttcttt ccatcaacaa actcaccttt atataaatca
      421 ccattatcgt atttaaatac tttttcttcc tttaatttct tattgttact cttattttta
      481 ttattattgt tatcctttat atgtatttct tctcttctgg cttcaataaa atctctatca
      541 tacctaaatc cgtttcttga tttaattttt tgattaatct tcttcttatt aataaatact
      601 ctagctagtt gagtaaatat aaatacagat gataagcata
//
LOCUS       DAEORZ010000332          638 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.339, whole genome shotgun sequence.
ACCESSION   DAEORZ010000332 DAEORZ010000000
VERSION     DAEORZ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.339"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          253 a           46 c           76 g          263 t
ORIGIN      
        1 aagaatgtat aaggtggtaa atttatataa aaaagaatat gagttgtaaa atattaaata
       61 atattgtact aattggaata attttaactt tattatagat tattttaata ccattagtac
      121 tgatggtgta ttttaaaatt ggtataagca ttacaaatac gaatatgcca actgtaatat
      181 gtaatataag tagctatata catatgtgct gtcccatata ttgtatcatt gtttatatta
      241 aaaagattat gtaaattggt atcaatcaat aaatcatttt cagaagaaat acctaaaaac
      301 ttaaaaaata cctatatgtg tatttagtta gatttgagat ttttacattt aattttatag
      361 tgacaatatt atattatttt tatgatgtct atttatatgc aattactata atatcagaac
      421 ttatagtatc atttgtttca ttatcaataa ggttttttac tttagttttg tcaattttat
      481 taaaaaaagt tattgatata aaagataaga aggataaaac tatatgacgg atttcaaaca
      541 taagagtgga tatttatata atagatattt tatagttaat tttattagat tttatttaat
      601 actgtaatat taatatcaca atatattaat agtgtatt
//
LOCUS       DAEORZ010000333          590 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.340, whole genome shotgun sequence.
ACCESSION   DAEORZ010000333 DAEORZ010000000
VERSION     DAEORZ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.340"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            217..585
                     /locus_tag="KRH39_003892"
     CDS             217..585
                     /locus_tag="KRH39_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBG8513752.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT          262 a           50 c          101 g          177 t
ORIGIN      
        1 gagttaattt aatatataat aaaagcatat caagtaaagc tttaaattaa agtaacttta
       61 tataaaaagt aatataaatt gataaaatta taaatttatt tatagtgaat ataggttaga
      121 ttttatagat acaaaaatag tatatatgat atgtagacca ttagcatgtt taaagtagca
      181 aaacatataa aataaaattt gaaaggaagt ataaaaatga caagaccatc aatatatcat
      241 agccaaggat acacttgtgc agaggcatta ataaagtctt ataatgagga acacaacaca
      301 gatataccaa tctcaatagg aagtggtatg ggggttggaa tgaatgttgg aagtgtttgt
      361 ggagctgtaa atgctgctgt tgttataata gggtatttaa atgggcgaaa tagtaattta
      421 gatgaaaata tagcaaggaa gtattcaaga gagctattga atagagtaag aaataaatac
      481 agctcagaaa tatgtgtatc tcttaaaaaa aataaagtaa cttgtgaaga aataataaat
      541 ttcacatatg atgctctaaa tgatatgcta aaaaatcaaa aataattcta
//
LOCUS       DAEORZ010000334          588 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.341, whole genome shotgun sequence.
ACCESSION   DAEORZ010000334 DAEORZ010000000
VERSION     DAEORZ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.341"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>588
                     /locus_tag="KRH39_003893"
     rRNA            <1..>588
                     /locus_tag="KRH39_003893"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          155 a          122 c          196 g          115 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtactag gtgtcggg
//
LOCUS       DAEORZ010000335          542 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.342, whole genome shotgun sequence.
ACCESSION   DAEORZ010000335 DAEORZ010000000
VERSION     DAEORZ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.342"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>542
                     /locus_tag="KRH39_003894"
     CDS             <1..>542
                     /locus_tag="KRH39_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG8513753.1"
                     /translation="CKKVFDYTSQSKTDLKKLNMYFLFSVFLIISSLYINIGANSRIL
                     ICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSMLLVATVSNLDNMNVLLSG
                     SLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIPMIANLLSFFVLYDYIIN
                     LVKISPERRTAFLVIVLLLI"
BASE COUNT          190 a           47 c           74 g          231 t
ORIGIN      
        1 atgtaaaaaa gtttttgatt atactagtca gtcaaagacc gacttaaaaa aactaaatat
       61 gtattttctc ttttctgttt ttctcattat ttcttcttta tatataaata taggagctaa
      121 tagtagaatc ttgatatgta tatttgtaat gttcgttttc tataagtcta actataaggt
      181 taatatatat aaatgtctgg taatctcatt tttttattgg ttaatattaa taggtataaa
      241 tggattaagt atgttattag tagcaacagt aagcaatttg gataatatga atgttttact
      301 tagtggcagt ttatatagat atgaagcaat attcttggga aagataatgt taggtatatt
      361 actttatgtt tataaggctt cttttaaaaa ttttaaaata agtaaaaaag agatatacat
      421 atgtattcct atgatagcaa atttattgtc attttttgta ctttatgatt atattattaa
      481 tcttgtaaaa atctctcctg aaagaagaac tgctttttta gtaatagttt tactattaat
      541 at
//
LOCUS       DAEORZ010000336          541 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.343, whole genome shotgun sequence.
ACCESSION   DAEORZ010000336 DAEORZ010000000
VERSION     DAEORZ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.343"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          229 a           48 c           47 g          217 t
ORIGIN      
        1 tttttattaa attttaatta ttgtattgaa gatttataag tataatttta ttgattctac
       61 ctttttaaat aactgtcgat ttattaaagt tatctatgta ctatattaaa caattaagta
      121 gttattaatt gaacttatta gtttattaaa aaactatgta tattactttg cagtaagact
      181 ttggaactaa atctataata gcaaatttaa atttattaaa tagattacct tataacattt
      241 catataaaat atattgtgaa tctcagaaaa ttactatttt tctttgagta ttttttgtaa
      301 gcagttctaa aagattcaat ttcataccta ttacttgaat cattcatcaa aataattcta
      361 atttaaaaaa taattctatc caaataatag atatatagga ctatgttata atataaaaaa
      421 atttatataa aagttaataa ctatacaaga ttgataaaga aaaagtcaaa atagataatt
      481 gattagcaaa atagatataa ttataatgaa gttaaatcta gcaatattct cctaaaatta
      541 a
//
LOCUS       DAEORZ010000337          522 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.344, whole genome shotgun sequence.
ACCESSION   DAEORZ010000337 DAEORZ010000000
VERSION     DAEORZ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.344"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            181..>522
                     /locus_tag="KRH39_003895"
     CDS             181..>522
                     /locus_tag="KRH39_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBG8513754.1"
                     /translation="MKNVNLKEINEKDVIVEEEINLKGMDKKDIIEKKEINKKEMDKK
                     DIIEKEEINKKGMNKKDIIEKEEINEKEIDKMIGKKDEIIDEKDIENFMDIEEDKKSI
                     KVSKKSTKKGET"
BASE COUNT          259 a           24 c          115 g          124 t
ORIGIN      
        1 aataacgtat gtaagaaccg taggaactac ggggatagcc tgtggagaat tagtaagaca
       61 tattttagta tgcaaaattc tatgaagcag gaaccctgtg actttagtca tgggaggttc
      121 agatgattaa actatttata aatagcgttt tataaattag tattttaaag ggggataatt
      181 atgaaaaatg taaatctaaa agagataaat gaaaaagatg taattgtgga agaagagata
      241 aatctaaaag gaatggataa gaaagatata attgagaaga aagagataaa taaaaaagaa
      301 atggataaga aagatataat tgagaaggaa gagataaata aaaaaggaat gaataagaaa
      361 gatataattg agaaggaaga gataaatgaa aaagaaatag ataaaatgat tggtaaaaaa
      421 gatgagataa ttgatgagaa agatatagag aattttatgg atattgaaga agataaaaaa
      481 agtatcaaag taagtaaaaa aagtactaaa aaaggtgaaa cg
//
LOCUS       DAEORZ010000338          514 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.345, whole genome shotgun sequence.
ACCESSION   DAEORZ010000338 DAEORZ010000000
VERSION     DAEORZ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.345"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(143..442)
                     /locus_tag="KRH39_003896"
     CDS             complement(143..442)
                     /locus_tag="KRH39_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513755.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
BASE COUNT          193 a           71 c           55 g          195 t
ORIGIN      
        1 aactaataca ttttacaaat tgttgagtaa tattttatga tataaatcag aagaggcact
       61 tctataatag aaagtgctca actatgaata agattaataa tatatttgcc ttagatatat
      121 agacttttat aaatattaaa gattaatcga atctgatgtt tgaaacttta tcccaattaa
      181 tcttccttga atagtcttct ggatgtgata atctaactat atgagttgaa ttatcatagc
      241 tgtaataaat tgtagtagta ccatcaactg gattcttttt gtatccttga tacacagcac
      301 tatttggtac tttcttttca acaaagctat atgcagatga agtatcaata gtttctattt
      361 tattaggaaa tataaacata taaaaagcta ctactataaa aatacatatg catgtaatac
      421 tataccattt aatccatttc ataaaattcc tcctacttta ccaaaatata ctattattat
      481 ttccatatat ttataatatt tatgcatacg ataa
//
LOCUS       DAEORZ010000339          514 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.346, whole genome shotgun sequence.
ACCESSION   DAEORZ010000339 DAEORZ010000000
VERSION     DAEORZ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.346"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(22..>514)
                     /locus_tag="KRH39_003897"
     rRNA            complement(22..>514)
                     /locus_tag="KRH39_003897"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          125 a          148 c          116 g          125 t
ORIGIN      
        1 acagtaggca attctcctta gaaaggaggt gatccagccg caccttccga tacggctacc
       61 ttgttacgac ttcaccccag ttattgattc caccttcgac gacttcttcc aaaaggttag
      121 ataatcggct tcgggcgtct ccaactcccg tggtgtgacg ggcggtgtgt acaagacccg
      181 ggaacgcatt caccgcagca ttctgatctg cgattactag taactccagc ttcatgtagg
      241 cgagtttcag cctacaatcc gaactgagag tagctttaag ggattagctc caccttacgg
      301 cttggcaacc ctctgtacta cccattgtag cacgtgtgta gccctaagca taaggggcat
      361 gatgatttga cgtcatcccc accttcctcc aggttatccc tggcagtctc tctagagtgc
      421 ccaacttaat gatggcaact aaagacaagg gttgcgctcg ttgcgggact taacccaaca
      481 tctcacgaca cgagctgacg acaaccatgc acca
//
LOCUS       DAEORZ010000340          473 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.347, whole genome shotgun sequence.
ACCESSION   DAEORZ010000340 DAEORZ010000000
VERSION     DAEORZ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.347"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>473
                     /locus_tag="KRH39_003898"
     CDS             <1..>473
                     /locus_tag="KRH39_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HBG8513756.1"
                     /translation="FAPANTDANNIEGQAILYQNEFLTLNGKKYYFGSDSKAVTGWRI
                     INNKKYYFNPNNAIAAIHLCTINNDKYYFSYDGILQNGYITIERNNFYFDANNESKMV
                     TGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNKFLTLNGKKYYFDNDSKAVTGWQT"
BASE COUNT          183 a           64 c           72 g          154 t
ORIGIN      
        1 tttgcacctg ctaatacgga tgctaacaac atagaaggtc aagctatact ttaccaaaat
       61 gaattcttaa ctttgaatgg taaaaaatat tactttggta gtgactcaaa agcagttact
      121 ggatggagaa ttattaacaa taagaaatat tactttaatc ctaataatgc tattgctgca
      181 attcatctat gcactataaa taatgacaaa tattacttta gttatgatgg aattcttcaa
      241 aatggatata ttactattga aagaaataat ttctattttg atgctaataa tgaatctaaa
      301 atggtaacag gagtatttaa aggacctaat ggatttgagt attttgcacc tgctaatact
      361 cacaataata acatagaagg tcaggctata gtttaccaga acaaattctt aactttgaat
      421 ggcaaaaaat attattttga taatgactca aaagcagtta ctggatggca aac
//
LOCUS       DAEORZ010000341          464 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.348, whole genome shotgun sequence.
ACCESSION   DAEORZ010000341 DAEORZ010000000
VERSION     DAEORZ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.348"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            70..291
                     /locus_tag="KRH39_003899"
     CDS             70..291
                     /locus_tag="KRH39_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513757.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT          216 a           26 c           85 g          137 t
ORIGIN      
        1 caaataaata tatgtacttt gggaaaaata aatataggaa gtattatatt tacagaggag
       61 gtagttttga tggatagatt aaaaagagat atgaaaaata tgaaagaaga tttaaaagaa
      121 ggtgttaaaa agacagctta tgttatgaaa aatacagctg aagatttgaa agatgatgca
      181 caaggtgcta tggtaaactt agaaatgaaa aaagatgaga tgatggaaag atatgaacaa
      241 aagaaatttt ctaaagaatt agagaagcaa atgaagaatg aaatgaaata agcttttatt
      301 tatgaataac aaattattgt attcatataa aaagtagcca gtaatattaa atagtttatt
      361 taaggaaata gactttctaa aaataactgt attagattta gaaagtctat ttcaaattag
      421 ttttataaat agacataagg ataaagtaga tgttatagaa ctag
//
LOCUS       DAEORZ010000342          459 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.349, whole genome shotgun sequence.
ACCESSION   DAEORZ010000342 DAEORZ010000000
VERSION     DAEORZ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.349"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            106..387
                     /locus_tag="KRH39_003900"
     CDS             106..387
                     /locus_tag="KRH39_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513758.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT          206 a           29 c           71 g          153 t
ORIGIN      
        1 agattagaaa ataatagtgt tactttcata ataaatacat ataaagatat ttttaagaat
       61 aaaatttatt attaaataaa aaataaagga gagtgattta atagtatggc atcaaaacaa
      121 acaccaagaa acaatttaga aaagttttta agaataagag tattatcaca tacaggcgat
      181 aaaatacata taaagttacc tgtaaatttt gcaaagagaa tgattgagaa taatgccttg
      241 gatttattta atggaaaaga tgatgtagta gatggtaaaa aagttactga aatattagtt
      301 aaggcatttg actatgattt aactggagaa gtagttcact tggagagaaa aaatggagac
      361 attattaaag tttcaattgt acaatagaat tattttatat gaattttatt tttgaatagt
      421 tataaataat tagaaatttg taaaaaagta taatatatt
//
LOCUS       DAEORZ010000343          454 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.350, whole genome shotgun sequence.
ACCESSION   DAEORZ010000343 DAEORZ010000000
VERSION     DAEORZ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.350"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..356
                     /locus_tag="KRH39_003901"
     CDS             <1..356
                     /locus_tag="KRH39_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG8513759.1"
                     /translation="DMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRIL
                     GNPLRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDP
                     DKYRDIVWNGECQSK"
BASE COUNT          197 a           53 c           89 g          115 t
ORIGIN      
        1 gagatatgcc aactataatg gcagggcttg cttgtggaga accaaacact ataggttttg
       61 aagtgttaaa aaattattca tcagcattcg tttcagcacc agactgggta tctgctaaag
      121 gtatgagaat attaggaaac cctctaagag gagatgagaa agtaatctct ggagaatctg
      181 gagcagttac aactggagca ttagtttcaa tccttgaaag tgaagattta aaagacttaa
      241 gagaagcttt gaaactagat gagaactcta aagtattatt aataagtaca gaaggagata
      301 ctgaccctga taaatataga gatatagttt ggaatggtga atgccaaagt aaataaaaga
      361 cttaaggaaa agaaaaactt aacaaataaa tgaaaaattt aaggaaaata aaaacttaaa
      421 taagtgaaaa ctttaaataa aataaaaact aaaa
//
LOCUS       DAEORZ010000344          434 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.351, whole genome shotgun sequence.
ACCESSION   DAEORZ010000344 DAEORZ010000000
VERSION     DAEORZ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.351"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            48..410
                     /locus_tag="KRH39_003902"
     CDS             48..410
                     /locus_tag="KRH39_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBG8513760.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT          185 a           27 c           81 g          141 t
ORIGIN      
        1 caatttatgg gtataacaat aaaaaattag aaaggaattt ttataaaatg attaaattat
       61 atggatatac aaaatgctct actgttaaaa aagctaaaaa ttggcttaag gaaaataatt
      121 tagaatttga agatatagat atggttcaaa atcctcctag taaagaggag ttaaaatcaa
      181 tttataagac tagtggatat gatataaaga aattttttaa tacaagtggt atgaaatata
      241 gagaattggg tcttaaagat attgtaaaaa ctgaatctga tgataaactt ttagaaatat
      301 tggtaagtga tggtatgctt attaaaagac cattactttt ggatggtaaa aatgtattat
      361 tgggctttaa agaagatgta tggaaaagta ctttgctaaa agaagattag aaagagatag
      421 gtgtgttgat taaa
//
LOCUS       DAEORZ010000345          427 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.352, whole genome shotgun sequence.
ACCESSION   DAEORZ010000345 DAEORZ010000000
VERSION     DAEORZ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.352"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..372)
                     /locus_tag="KRH39_003903"
     rRNA            complement(<1..372)
                     /locus_tag="KRH39_003903"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          109 a          106 c           71 g          141 t
ORIGIN      
        1 aggactatta ccttctgtgg tgggtctttc caaacctctt caattacgat acctcttcgt
       61 tatgatatgt ccgcaacccc aataaagaaa actttattgg tttgggctaa tccgcgttcg
      121 ctcgccgcta cttacggaat cgaatttctt tctcttcctt caggtactta gatgtttcag
      181 ttccctgagt tcccctcatt aagctatgta ttcacttaat gatacttaga cattactcta
      241 agtgagtttc ctcattcgga aatcttcgga tcaaagttta cgtgcaactc cccgaagctt
      301 atcgcagctt atcacgtcct tcatcggctc ctagtgccaa ggcatccgcc ctgcaccctt
      361 aataacttga ccagttaaaa aggtttgata gattagaagc tatcaacttc gataatttta
      421 aaaagtt
//
LOCUS       DAEORZ010000346          419 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.353, whole genome shotgun sequence.
ACCESSION   DAEORZ010000346 DAEORZ010000000
VERSION     DAEORZ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.353"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          136 a           63 c           29 g          191 t
ORIGIN      
        1 tagttatttc ttttattaaa tatctttact ttttttactt attatgtctt ctagctttac
       61 atttggatta tggtatctct ttctattttt agtcacatct ttctattgaa ttacttgctt
      121 aaaacaccaa tgcacacatg ccagataatg ttcacaatta tgtagaaatc ttgctctacc
      181 ttcttcaatg ttgatattat ctctcctagt tgatattatc tttcctaaaa tttcttttaa
      241 ttaaaaaaca acattagttg atatatcaag tatattttaa gcaactgaaa aaaacaataa
      301 taattgatat atcaactaat taatgtagat taattcattt ttataattta tatacttttt
      361 aattaatcta ccttatttaa ttaaaatcct tcctttctat ttatccattt ttaacttaa
//
LOCUS       DAEORZ010000347          415 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.354, whole genome shotgun sequence.
ACCESSION   DAEORZ010000347 DAEORZ010000000
VERSION     DAEORZ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.354"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>415
                     /locus_tag="KRH39_003904"
     CDS             <1..>415
                     /locus_tag="KRH39_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG8513761.1"
                     /translation="LFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRGTSDMKGQVS
                     AMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYVVIGESSELN
                     LKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMA"
BASE COUNT          143 a           50 c          101 g          121 t
ORIGIN      
        1 ttatttgacg gtcatataga tacagttcca gttccagatg caagtaaatg gagtgttcct
       61 ccttttgaag cgagaatagt agatgggaaa atatatggta gaggaacttc tgatatgaag
      121 gggcaagtaa gtgctatgat ggctgctgtt tcatactttg cagaagatac taacaaagac
      181 tttgaaggtg aattgtatgt agcaggtgtt gttcatgaag aaatatttga aggtgtttca
      241 gctagagaga taagtaaggc tgttcaacct gattatgttg taataggtga atcttcagaa
      301 ttaaacctaa agataggtca aagaggtaga ggagaaatag tagtagaaac ttttggtaaa
      361 ccagctcact ctgctaatcc agaaaaaggt ataaatgcag tttataaaat ggcta
//
LOCUS       DAEORZ010000348          391 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.355, whole genome shotgun sequence.
ACCESSION   DAEORZ010000348 DAEORZ010000000
VERSION     DAEORZ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.355"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          168 a           32 c           36 g          155 t
ORIGIN      
        1 tatcatacct atataatatt cttatatgta tctcatttgt agttattaaa tataagatat
       61 atttaattga cttttcaaga taaattttta tattaatctc ctctgatttc tcattttttg
      121 aatacactaa aatttccaat taaatcaatt atatatttat aaagttttaa aaatatagta
      181 atgtatatat tttttgtgaa aaacttgctt aaatttagcc aaaaaaatag ataattcata
      241 gtaaaaaata aagtttagta aaaaatttta atagaactat aaaagtctgt tttagtaaaa
      301 gatttaaaat tctaatatta agtgaaattt tgggtgaatt aaataagcga aactataata
      361 gaaatagaat atatggaaaa atacatctct a
//
LOCUS       DAEORZ010000349          347 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.356, whole genome shotgun sequence.
ACCESSION   DAEORZ010000349 DAEORZ010000000
VERSION     DAEORZ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.356"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          163 a           29 c           35 g          120 t
ORIGIN      
        1 tagttgtttt gctatttatt atttgtttaa tatttctaat ttataaaaaa aaattataaa
       61 ttttaaaaat tataaatgac attttaacta gaattaattg taaaaaacac tatataatac
      121 tcaattttca acattctata caattataca gaaattatac agcataataa tcaccgaaat
      181 aacaaatttt aaaaattaag caaaactttt tgtcgaagtt caacgtctat atatatgtag
      241 tgtaaatttt tgggataaaa tactacaaaa atataaatgt aaaaatatag taatattgga
      301 ggtcgaacga tgagaaataa aaagaaaaaa ataaatagaa agaagtt
//
LOCUS       DAEORZ010000350          341 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.357, whole genome shotgun sequence.
ACCESSION   DAEORZ010000350 DAEORZ010000000
VERSION     DAEORZ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.357"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            87..>341
                     /locus_tag="KRH39_003905"
     CDS             87..>341
                     /locus_tag="KRH39_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8513762.1"
                     /translation="MVLGSKSKVSVKFKEKPDADSITLNYKCYDMPLDTTLNYNQSTG
                     AYEGTINYNQDPEYLNVWELQGITINSKNNPKTLNRQDLEK"
BASE COUNT          149 a           55 c           52 g           85 t
ORIGIN      
        1 tcgtcgcacc tacaatggca tttgctactg aatctaatgc tatggaaaat aacgctgatt
       61 taaatataaa cttagagaaa aaaagtatcg ttttaggtag caaatcaaaa gttagtgtca
      121 aatttaaaga aaaaccagat gcagatagca ttacattaaa ttataaatgc tatgacatgc
      181 cattggatac aactctaaat tacaatcaat caactggggc atatgaagga actatcaatt
      241 ataaccaaga cccagaatat ctaaatgttt gggaactaca agggataaca ataaacagca
      301 aaaataatcc taaaacttta aacagacaag acctagaaaa g
//
LOCUS       DAEORZ010000351          308 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.359, whole genome shotgun sequence.
ACCESSION   DAEORZ010000351 DAEORZ010000000
VERSION     DAEORZ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.359"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            182..295
                     /locus_tag="KRH39_003906"
     CDS             182..295
                     /locus_tag="KRH39_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG8513763.1"
                     /translation="MIMGRHLWNPSYFVATVSENTEEQIKKYINNQKKQVK"
BASE COUNT          131 a           29 c           50 g           98 t
ORIGIN      
        1 ctcaaaaatg ataaaagtta agaacttaca gttcttatat atgttattta aaaataaata
       61 taaaaaacct tcaaaatact tgttttaagt aattgcatat tttaatatta tgataagtga
      121 tgaaaaatgg aagttactca tggtagagga tatacatata gtatgtcaaa tatagacatc
      181 cattattatg gggagacatc tttggaatcc tagttatttc gttgctactg tatcagaaaa
      241 tacagaggag cagataaaaa aatatattaa taatcagaaa aaacaagtaa agtgaggtga
      301 tagtagtg
//
LOCUS       DAEORZ010000352          302 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.360, whole genome shotgun sequence.
ACCESSION   DAEORZ010000352 DAEORZ010000000
VERSION     DAEORZ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.360"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..262
                     /locus_tag="KRH39_003907"
     CDS             <1..262
                     /locus_tag="KRH39_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513764.1"
                     /translation="IQKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCF
                     ICSPVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT          141 a           25 c           33 g          103 t
ORIGIN      
        1 tattcaaaaa gataagtatt ataaaaatga tatacaaaat aacaatgaaa atataaacga
       61 ttgtaaaaag caaataagtt ttggtgataa taaggtaaaa aaaataacaa aaacttttgg
      121 attttcctgt ttcatttgtt ctccagttgg aattatttat gaagatatga aaaatataaa
      181 tataaaaatt gaacatacat caataaatga attaaatcca gaagaattat ttgtaaatac
      241 atcgctttta ttgtattatt aagtatttta aagctaaaac ttaaacttat taaaatagca
      301 tt
//
LOCUS       DAEORZ010000353          300 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.361, whole genome shotgun sequence.
ACCESSION   DAEORZ010000353 DAEORZ010000000
VERSION     DAEORZ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.361"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          147 a           20 c           32 g          101 t
ORIGIN      
        1 aattaccaaa aatataaaaa aaaaaggtta aaataataaa atatattgaa aattaccatt
       61 tgtatatgta taatatgaat tatacaaatg gtaatttgat gagaggtgat atgttgaaat
      121 aagaaactga attaattaat aaatatatag aaagttttga aaacccttaa ttgagatata
      181 aacaatatgt acaaaatttt ttgcaagtaa aattaccaaa tgtataaaat aaatcagtaa
      241 catagataat tttatacttt aatacattag attatctaat ttaattataa tggtcaaaaa
//
LOCUS       DAEORZ010000354          298 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.362, whole genome shotgun sequence.
ACCESSION   DAEORZ010000354 DAEORZ010000000
VERSION     DAEORZ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.362"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>298
                     /locus_tag="KRH39_003908"
     CDS             <1..>298
                     /locus_tag="KRH39_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513765.1"
                     /translation="IELFKLNYEEEINNILDSIEYNICEYILYDYSNEFLDNLIYTNN
                     QVDFIKEGQFVKLLNKKIDKLKSLSKDKIAVIYKSKANLSCLDMYIDLKNKYIN"
BASE COUNT          140 a           17 c           37 g          104 t
ORIGIN      
        1 atatagagtt atttaaatta aattatgaag aagaaattaa taatatatta gatagtatag
       61 agtataatat atgtgaatat atattatacg attattctaa tgaattttta gataatttaa
      121 tatatacaaa taatcaagtg gattttatca aagaaggaca gtttgtaaaa ttattgaata
      181 aaaaaataga caaactaaaa tcattaagca aggataagat agcagttata tacaaatcaa
      241 aggcaaattt atcgtgctta gatatgtata ttgatctaaa aaataaatat attaattt
//
LOCUS       DAEORZ010000355          250 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.365, whole genome shotgun sequence.
ACCESSION   DAEORZ010000355 DAEORZ010000000
VERSION     DAEORZ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.365"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..215)
                     /locus_tag="KRH39_003909"
     CDS             complement(<1..215)
                     /locus_tag="KRH39_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513766.1"
                     /translation="MNNMENKKNFLRKIVKLRSIFLSIFGVFLFITATTGIQTVDRYV
                     PTWLYITLGVSATISLVLYVISFILKI"
BASE COUNT           93 a           40 c           22 g           95 t
ORIGIN      
        1 tttattttta aaataaagga aataacgtat aaaactaatg atatagttgc tgacactccc
       61 aatgttatat ataaccatgt tggaacatat ctatcaacag tttgaatacc tgtagttgct
      121 gtaataaata aaaacacacc aaatatgctt agaaatatgc ttcttaattt tactattttt
      181 cttaaaaaat ttttcttatt ttccatgtta ttcatatcca tcccaccttt ttaaataata
      241 gcaacaactt
//
LOCUS       DAEORZ010000356          244 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.366, whole genome shotgun sequence.
ACCESSION   DAEORZ010000356 DAEORZ010000000
VERSION     DAEORZ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.366"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT           90 a           26 c           21 g          107 t
ORIGIN      
        1 tacagggtat ataggattga tgtactattt actaatatta cattttcaac tgattcaacc
       61 cctcttttac ttacataatt taagtatatt tttctggatt tttaaattta tgtactttaa
      121 aatggatata cttattttca ttgattttgt aagaattaat aaaaaatacg attacactac
      181 aaaattagaa tgtcaatgtt tataaatata aaataatatt ttttatttta attattacat
      241 ccat
//
LOCUS       DAEORZ010000357          239 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.367, whole genome shotgun sequence.
ACCESSION   DAEORZ010000357 DAEORZ010000000
VERSION     DAEORZ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.367"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>239
                     /gene="tuf"
                     /locus_tag="KRH39_003910"
     CDS             <1..>239
                     /gene="tuf"
                     /locus_tag="KRH39_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG8513767.1"
                     /translation="PRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLA
                     KTGSVKAHTKFTAEVYVLKKEEGGRHTPFFDGYRP"
BASE COUNT          106 a           32 c           57 g           44 t
ORIGIN      
        1 ccaagaaaag tagtagtaac aggagtagag atgttcagaa aattattaga ccaagcacaa
       61 gcaggggata atataggagc attattaaga ggagtacaaa gaaacgagat agaaagagga
      121 caagtactag caaagactgg atcagtaaag gcacacacaa agtttacagc agaagtatat
      181 gtacttaaaa aagaagaggg tggaagacat acaccattct ttgatggata tagaccaca
//
LOCUS       DAEORZ010000358          231 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.370, whole genome shotgun sequence.
ACCESSION   DAEORZ010000358 DAEORZ010000000
VERSION     DAEORZ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.370"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>231
                     /locus_tag="KRH39_003911"
     CDS             <1..>231
                     /locus_tag="KRH39_003911"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513768.1"
                     /translation="KREVDSIEIQINDDIDKLKYREQELKQEVQLAEGEPIPFEIVKD
                     IMDKFKEVFFNMSTSQQRKQLIHLLVSQITMN"
BASE COUNT          113 a           22 c           41 g           55 t
ORIGIN      
        1 aaaaaagaga agtagatagt atagagatac aaataaatga tgatatagat aagttgaagt
       61 acagagaaca agagttaaaa caagaagttc aactagcaga aggggaacca ataccatttg
      121 agatagtaaa agatataatg gataagttta aagaagtatt ctttaatatg agtacaagtc
      181 aacaaagaaa acaattaata catctactag tttcacaaat aactatgaat g
//
LOCUS       DAEORZ010000359          228 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.371, whole genome shotgun sequence.
ACCESSION   DAEORZ010000359 DAEORZ010000000
VERSION     DAEORZ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.371"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     regulatory      <1..69
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT           91 a           33 c           34 g           70 t
ORIGIN      
        1 gaaatctagt gacgcaaagc tatagggact aaggtttata tacatgaact atgtcagcca
       61 gttgccaaaa agagtcctag gtgtattgta tacctaagaa aagtctagaa tgactgcctt
      121 tttggcagtc attttgttat gaaaaaatca ctttatttca gtagaaaata cctataatta
      181 acatacttta ccaaattata acataaaata aagaaaaatt atcttaaa
//
LOCUS       DAEORZ010000360          227 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.372, whole genome shotgun sequence.
ACCESSION   DAEORZ010000360 DAEORZ010000000
VERSION     DAEORZ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.372"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>227
                     /locus_tag="KRH39_003912"
     CDS             <1..>227
                     /locus_tag="KRH39_003912"
                     /inference="COORDINATES: protein motif:HMM:NF012632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513769.1"
                     /translation="LKNNFTEYANNYKIVLGDTSFILKNDNTLWGSGSSSYGQLGLGD
                     TAGKRIFTRMNVENIKDVVCGIRHVVVLKNDG"
BASE COUNT           85 a           19 c           50 g           73 t
ORIGIN      
        1 ctaaaaaata atttcacaga atatgcaaac aattataaaa tcgtattagg agatacttct
       61 ttcatattaa agaatgacaa tactttatgg ggtagtggta gtagttccta tggtcaatta
      121 ggtttaggtg atactgctgg caaaagaata tttactagaa tgaatgtgga aaatataaaa
      181 gatgttgtat gtgggataag gcatgtggtt gtattgaaaa atgatgg
//
LOCUS       DAEORZ010000361          227 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.denovo.373, whole genome shotgun sequence.
ACCESSION   DAEORZ010000361 DAEORZ010000000
VERSION     DAEORZ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.denovo.373"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            167..>227
                     /gene="rrf"
                     /locus_tag="KRH39_003913"
     rRNA            167..>227
                     /gene="rrf"
                     /locus_tag="KRH39_003913"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT           87 a           30 c           32 g           78 t
ORIGIN      
        1 ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta ctaacttcaa
       61 ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact attgacattt
      121 aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg gttataatag
      181 caaagaggat acacctgttc ccattccgaa cacagaagtt aagctct
//
LOCUS       DAEORZ010000362        14729 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.guided.105, whole genome shotgun sequence.
ACCESSION   DAEORZ010000362 DAEORZ010000000
VERSION     DAEORZ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14729
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.guided.105"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(214..2301)
                     /locus_tag="KRH39_003914"
     CDS             complement(214..2301)
                     /locus_tag="KRH39_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBG8513770.1"
                     /translation="MIKVVYMRKNKKTRKMIIFGFLMCLLIVIIVFLAKFILDGLNKN
                     KPDEVFKQYMSFANKKQYEKMYDLLDEKSKSENKKEDFVLRNKKIYEGIDAHDISIKI
                     NDIKKNKNNDKVINYDSKMDTLAGEISFSNEVLLTRDRQKNYKIKWQSNVIFPELEED
                     NTVRVSKLKGKRGRILDRNGVMIAGQGLASMIGLVPGKMSDNIEDLKKLSTLLNVSVE
                     QIEKKLNASWVKENSMVPIKTIEKIKENTDGTVKEEDKELQESLLSIPGVKISNTEVR
                     VYPFGEKTGHLTGYVQNVNADILKEKEGKRYNDNSIIGKIGLENLLEDRIRGIDGYEI
                     IIADRYGDKKETLVTEPKVDGEDVKLTIDSKLQSKLYDQMKNDKGCVVVMNPKTGEVL
                     SLVSSPSYNPNEFILGMSEDRWNELNKNENKPMYNRFKARLCPGSSFKPVTAGIGITT
                     GKINPNENFGHSSLSWQKDSSWGSYKVTTLKDYGNTANMKNALIYSDNIYFAKAALKI
                     GEDVLAKELLKLGFDESMPFEFGLSSSKFGTDNKFETEIQLADTGYGQGKLLVNPVHT
                     ASIYSAFVNDGDMIKPHLEFKKAEFWKKGVFSKEAVKIIRDDLLQVVESPDGTGHSAM
                     IKGISIAGKTGTAEIKASKDDVTGTELGWFNAFTVDENSDKQYLIIAMIEDVKSRGGS
                     HYVIPIVKSVFED"
     gene            2662..3240
                     /locus_tag="KRH39_003915"
     CDS             2662..3240
                     /locus_tag="KRH39_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8513771.1"
                     /translation="MSTKGEQTKQFIIDTSIMLFSEKGYSNVTMKDICEACNLSRGGL
                     YRHFNSPKEIFISILNAHKYYSQNLLDKGIKENISAKHMLEYFFQQQKADINGKYCGF
                     DFAIHEFAFVEKDQRDYMNKRYSSAITMLSNLIDYGQSKSEFKKCDSKSVAIHIMFLF
                     DSMKTSASILKLTNKDIDNQINIIKDMVILYE"
     gene            3233..3892
                     /locus_tag="KRH39_003916"
     CDS             3233..3892
                     /locus_tag="KRH39_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HBG8513772.1"
                     /translation="MNKQKFHKIDLQNWERYTHFYYFSKMTNVGFNLTIEIDITNLLK
                     ETKKRNIKFYPAYLYTITTCLNQYRELKIAYDEDELGYWNTLTPLYAIFHDDNKTFSS
                     IWTEYDENFSIFYENYLADVKEYGKNHGFLAKPNPPRNCYTVSCLPWISFKSFTVHNH
                     GLENYFFPTIEAGKYYQKYEKILLPLSITVHHATTDGYHVSCFIDYIQKMCFNTKIWL
                     H"
     gene            complement(4104..5978)
                     /locus_tag="KRH39_003917"
     CDS             complement(4104..5978)
                     /locus_tag="KRH39_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8513773.1"
                     /translation="MNKYNKIANELIEIIGENNIISITHCATRLRVMVKDREIINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVV
                     FCFIATCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVT
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(5983..7647)
                     /locus_tag="KRH39_003918"
     CDS             complement(5983..7647)
                     /locus_tag="KRH39_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBG8513774.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIVSRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKAVRNQPGMEKMLYELKENTFKKYDCMTVAEAAGISYDQLGEFIGEDGFFSMV
                     FDFKYADLDVASGSEWFKRISWTISDLRKYILDSQMYVQQHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRNTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWSDKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDNVIAYRRQLDLVVIDCY
                     YNFSDKPVEIKTEEKYDIVFQNLDDINLLNKTVSLKPFQAVLLKVR"
     gene            complement(7661..8482)
                     /locus_tag="KRH39_003919"
     CDS             complement(7661..8482)
                     /locus_tag="KRH39_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG8513775.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKLQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIQRLQERLGITQKET
                     ICFGDGQNDIVMFQASDITIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(8581..9345)
                     /locus_tag="KRH39_003920"
     CDS             complement(8581..9345)
                     /locus_tag="KRH39_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8513776.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKKMKMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(9342..10091)
                     /locus_tag="KRH39_003921"
     CDS             complement(9342..10091)
                     /locus_tag="KRH39_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8513777.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYYAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(10373..11425)
                     /locus_tag="KRH39_003922"
     CDS             complement(10373..11425)
                     /locus_tag="KRH39_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513778.1"
                     /translation="MNVINVFNSNLKRNINKRIVFLVTLLFPIIIVVLGVLANYISKP
                     SFNIGILDEYKNECILKESEKRTYENTIMALENTQGINTEIADFKTIKTDIITGKYSA
                     VICFNKNGFELYSIKDKNTNDTLKYLIETYKKDPVPVDISKLQENTLGVLQRIIAFIV
                     LFLMITSTVTASMIIKDKNSGTFTRVLYSPQNIRGYVLGNMLYNFTITYFQYIVSISI
                     IKLFNIDIGMDYIDLILMGVWISALATSFGTFVSSLFNKEMHANLFSTCISLILSLIG
                     GSFISVEKMPTMLQKISIISPTQWFISFVTYIEERDGSFFNMNYICILTFIIIILALL
                     AMKFTQRSKYSSNRNY"
     gene            complement(11425..12165)
                     /locus_tag="KRH39_003923"
     CDS             complement(11425..12165)
                     /locus_tag="KRH39_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8513779.1"
                     /translation="MRCFLTLFKLGIKRRSKDFFILFYNIVFPVIIIVLLGYLTSKSY
                     GKEFTSYNYYTIVTIPFCVLMGIISVSYVAQDEKISNTSYRYMISPISIVDLVISKLF
                     SCTTVFSIYNLITLVVLRGIFHMSFGGKFLVVIFFLMCESIAVVSMGLFLGLVCKNLD
                     MLRNIINIPIVIFGFLGGVFFPVSSLNPVISLIINISPLTWINRGIVACIYDNNLQLL
                     FNISCIFVAVSIFMTILTIKFFKKEVFI"
     gene            complement(12170..12928)
                     /locus_tag="KRH39_003924"
     CDS             complement(12170..12928)
                     /locus_tag="KRH39_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8513780.1"
                     /translation="MSNVLLEIKNLSKKYNNKEVVSRESFKLLEGDILGFIGPNGAGK
                     STSINMLTTIVSPDSGSIYYKGKDIKNQQNIFKSSLGVVPQELAIYEDLSAYDNVKFF
                     CSLYGFKGNELKERTKYALEFVGLWESHKEFPSKFSGGMKRRLNIACAIAHSPQILIM
                     DEPTVGIDPQSRNKILEAIKGINNNGTTVIYTSHYMEEIETLCNRIVLIDKGVIIEDL
                     DKSLYKNKYLKFGCKTLEDIFLYLTGTDLRDLEE"
     gene            complement(13033..13755)
                     /locus_tag="KRH39_003925"
     CDS             complement(13033..13755)
                     /locus_tag="KRH39_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8513781.1"
                     /translation="MIRTLILYYLNIKPTHGYEIQKFLQVSGADRWTKIQSGSIYYAL
                     AKLEKDGGVRVLKEEKTGARIRKIYEITQSGKLELREEIQKELQMPIVPTGSNKFLLH
                     NILDVLPKDTLQKNLEKHIKYLIEQKKYWENWKEIKKIDKKSLATEKIAFDMTIDNLN
                     YQILWHEEILNNIDKYISVGCETQNIIKSIDFSNIEEDFLFTSDTTNELLEVQRLRDE
                     IINNPDKAIENVDKIILKLQNK"
     gene            complement(13856..14461)
                     /locus_tag="KRH39_003926"
     CDS             complement(13856..14461)
                     /locus_tag="KRH39_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8513782.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGENIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKVISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            14640..>14729
                     /locus_tag="KRH39_003927"
     CDS             14640..>14729
                     /locus_tag="KRH39_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBG8513783.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNG"
BASE COUNT         5074 a         2510 c         1541 g         5604 t
ORIGIN      
        1 tggcaactct ccctcacatc ctcaattgtt gccactattt aattttttgt atgaagttaa
       61 atatattttc atcatttcaa caataaaaaa gaggtctatc tctattttga aacaacctct
      121 ttaattattt tagagtttac taagaagtct aaaaacaatg tatctaaaat attattctca
      181 actgagtaag attaatttcc aaattgagta aatttaatct tcaaatactg atttaacaat
      241 aggaataaca taatgacttc ccccacgact ctttacatct tcaatcatag caattattag
      301 atattgttta tcactatttt catctacagt aaaagcatta aaccatccaa gttctgtccc
      361 tgtaacatca tcttttgatg ctttaatttc tgctgtacca gttttcccag caatgctaat
      421 tcctttaatc atagcactat gacctgtacc atcagggctt tcaacaactt gaagtaagtc
      481 atcacgaata atttttacag cctctttaga aaacactcct ttcttccaaa attcagcctt
      541 tttaaattca agatggggtt ttatcatgtc tccatcattt acaaaagctg aatatattga
      601 tgccgtatgt actggattta caagaagctt accttgtcca tagccagtat ctgctaattg
      661 tatttcagtc tcaaatttat tgtcagtgcc aaattttgaa gaagacaatc caaattcaaa
      721 tggcatactt tcatcaaatc caagcttcaa caactctttt gctaatacat cttcacctat
      781 ttttaaggct gccttagcaa aataaatatt gtcagaataa atcaaagcat ttttcatatt
      841 tgcagtattt ccataatctt ttaacgtggt taccttataa cttccccaac tagaatcttt
      901 ttgccaactt agactgctgt gtccaaagtt ttcatttgga ttaatctttc ctgttgttat
      961 accaatacca gcagtaactg gtttaaaaga agacccagga cataatcgtg ctttaaatct
     1021 attgtacatt ggtttatttt catttttatt gagttcattc catctatctt ctgacattcc
     1081 caatataaac tcatttggat tgtacgacgg actacttaca agtgataaaa cctctcctgt
     1141 tttaggattc ataactacta cacaaccttt atcatttttc atttggtcat atagtttact
     1201 ctgtagtttt gaatctatag ttagctttac atcttctcca tctacctttg gctcagttac
     1261 taaagtttct ttcttgtcac catatctatc agctattata atctcatatc catcaatacc
     1321 acgtattcta tcttccagta aattttctaa accaatctta ccaattatac tattatcgtt
     1381 gtacctttta ccctcttttt cctttagtat atctgcattt acattttgaa cataaccagt
     1441 taaatgacca gttttttcac caaatggata tactctaacc tctgtattgg atattttaac
     1501 tccaggaata ctaagaagtg actcttgtag ttccttatct tcttctttta cagtaccatc
     1561 tgtattttct tttatttttt caatagtttt tataggtacc attgaatttt cttttaccca
     1621 agatgcattt aatttctttt caatttgctc aactgataca ttcaataaag tagataattt
     1681 ttttaagtct tctatattat cactcatttt tccaggaact aatcctatca tagatgcaag
     1741 accttgacca gcaatcatta caccgttcct atccagaata cgtcctctct tacctttcag
     1801 ctttgatacc cttacagtat tatcttcttc aagttctgga aatatgacgt ttgactgcca
     1861 ttttatttta taatttttct gtctatctct agttaatagt acttcatttg aaaatgaaat
     1921 ttcacctgct aaagtatcca tcttagaatc atagtttatt actttatcat tatttttatt
     1981 ttttttaata tcattaatct taatacttat atcgtgtgca tcaattcctt catagatttt
     2041 tttatttctt aaaacaaaat cttctttttt attttcagac ttgctctttt catctaataa
     2101 atcatacatt ttctcatatt gttttttatt tgcaaaagac atatactgct taaacacttc
     2161 atctggtttg tttttattga gtccatctag tatgaatttt gctaaaaaaa caataataac
     2221 tataagcaga cacattagaa aaccaaaaat tatcattttt cttgtttttt tattttttct
     2281 catataaacc acctttatca tttaatatat tacatacgta agctttaagg ttaaaatact
     2341 cttatagaca tagctaatct atgtttttat tatgttttta agtatctcat aaaaatatgt
     2401 aagcagtata attgatagta atttatatat tacacatgta agttttaaaa gtcaataata
     2461 ttatcattat ttaattattt tttacatcaa cttaaaaata aatcagttcc attatattct
     2521 tttgtaccct tcaactattt tataaattaa ccttattcta ttgtaattat tttatggtat
     2581 ataactttaa cttgacaaaa cgacacaagt gtctcataat taaaaatgac acttatgtcg
     2641 ttaaaaagga ggttaaaaca tatgtcaaca aaaggagaac aaactaaaca atttatcatt
     2701 gatacatcaa taatgctatt ttctgaaaaa ggatatagta atgtgacaat gaaagatata
     2761 tgtgaagcat gtaatttaag ccgtggtggc ctatatagac actttaattc accaaaagaa
     2821 atttttatta gtatattaaa cgctcataaa tactactctc aaaatttgtt ggataaaggt
     2881 atcaaggaaa atatatcagc taagcatatg ctagaatatt ttttccaaca acaaaaggct
     2941 gacataaatg gcaaatattg tggatttgat tttgcaatac acgaatttgc ttttgtagaa
     3001 aaagaccaac gtgattatat gaataaaaga tacagttcag ctatcacaat gctttcaaat
     3061 ttaatcgatt atggacaatc taaaagtgaa tttaaaaaat gtgattctaa atcagttgca
     3121 atacacataa tgttcttatt tgatagcatg aaaacatctg cttccatttt aaaacttaca
     3181 aataaagaca ttgataatca aataaatatt ataaaggaca tggtgatttt atatgaataa
     3241 acaaaaattc cataaaattg atttacaaaa ctgggaacga tatacacatt tttactactt
     3301 ttcaaaaatg acaaatgtag ggtttaattt gactatagaa atagatatta ctaatttgct
     3361 taaggaaacg aaaaaaagaa atattaaatt ttatccagcg tatttatata caataactac
     3421 ttgcttaaat caatatagag aactaaaaat cgcatatgat gaagatgaac tcggatattg
     3481 gaacacgtta acgccacttt atgcgatttt tcatgatgat aataagactt tctcatcaat
     3541 atggacagaa tatgatgaaa atttctcaat attctatgaa aattatttag ctgatgttaa
     3601 agaatatgga aaaaatcatg gttttctagc aaaaccaaat ccgccaagaa actgctatac
     3661 tgtttcttgc cttccatgga ttagctttaa gagttttact gtacataatc atggtcttga
     3721 aaattatttt ttccctacaa ttgaagcagg taaatactat caaaaatatg aaaaaatact
     3781 tcttcctctt tctataacag tgcatcacgc aactactgat ggataccatg taagttgttt
     3841 tattgattac atacaaaaaa tgtgctttaa tactaagata tggctacact aaaaatttat
     3901 acaatataaa aagaactgtc gcatgagcaa attaaatttg ctatgagaaa gctctttttt
     3961 acttaccaat agttaatatg agatgaatta tttcttatac tttgaactta ccaatagtta
     4021 atatgaaatg aattatttct catactttga aaatattgaa ttgattttga taatcttctt
     4081 ttataatcat tacgtgtcac ctactactca acacatattc tttgacctat ttttacatcc
     4141 ttgtttatat ccaaaatatt taccttctta ccatctggaa acaataccat tgtcaaagta
     4201 gactttccta attcgtgtat tctctttaaa tcaacattaa caagaacatc accctgttta
     4261 accatatctc cttgtttaac agtaacatca aagccatctc catttaatgc aactgtatca
     4321 attccaacat gaattaatac ttctccatta gttcctttaa tcccaaatgc atgtccactt
     4381 gaaaatactg tttctaattt acctgaaata ggggaaacaa tttttccatc tataatatca
     4441 atagcaaaac catcacctaa cattttttta gaaaaggttt catcaggtac attttctaat
     4501 gcaacaatct tacctttagc tggagacaca aagttttcat tattttcatt aaacactaat
     4561 tcttcgtctt catcttcatt tatagcttct ttaggtactc caaataaaca tgttgcaata
     4621 aagcagaata caactacaaa tattgatact aataagtatg tgattaaatt atatccatca
     4681 tagatataca ttaaataaga aggtaaaact gctaatccat atgaattaga ttgaatatgt
     4741 aaaattgata atattgctgc tcctaaacct gaagatgcac acataataat caaaggcttt
     4801 aaattatatc gaagctgaat cccgaataaa acaggttcac ttataccaaa aagttgagat
     4861 agcattgctc caactgcagt ttgtttaact tgattatttt tagatttttt cataaatgct
     4921 agacatgttc caatattcgc aaatccatac attgcacaaa gagtaataag tgcattaaat
     4981 cctgtatttg ctaatagtga tgtttcaatc attacataag tatgatgtaa gccagtaata
     5041 acagctaatg gatatgtaaa tccaataata aatcctccaa ttcctaaagg taaaccaatc
     5101 aataagctga tgatatcaac catttttaat tctattgtat gcattatagg ccctacacct
     5161 aacaaaataa ctaacattgt tattagcata acaaagaatg gtgtgaaaat aaggtctaat
     5221 acattaggca tatgtttacg gaaccatttt tcaagattag ctccaataaa tgctgtaaca
     5281 atagctgtta aaacacttcc ttgacagcct acaataggaa taaatccaaa tgccataatt
     5341 gcttcaactt catttgatgg gtcagcaaca gaatatgcat taggaagtat tggggaaact
     5401 aacataagtc caataacaag tccaataaca ggtgttcctc caaatacctt aaatgctgac
     5461 catacaataa ttgctggtaa aaaagcaaat gctgtttctg taagaaccga aacaagtgtc
     5521 tgaatataca atggaatatt tgcagaagac attccaaata atccaagtac attatcatta
     5581 aacagacaac cctttaatcc caagaataat cctgttgccg caataactgg aataatagga
     5641 acaaatatat ctgctagagt tctcataact ttcttaaatc cagtttcatt atttttaact
     5701 aattcatctt gttctttttt tgataaagtt ttcaatccca ttttttcaac ttctgcataa
     5761 actttattga ctattcccgt tcctaaaata atttgatact gtccagaagt aaagaaaaca
     5821 cctttaacct cattaacctt ttccactttc ttatcattaa taatttctct atctttaacc
     5881 atcaccctta atcttgtggc acaatgtgta atagaaataa tattattttc accaataatt
     5941 tcaataagct catttgctat tttattatat ttattcattt ttctacctca ctttcaataa
     6001 tactgcttga aatggtttca aactcactgt tttatttagt agatttatat catctaaatt
     6061 ttggaataca atatcatatt tttcttctgt ttttatttct acaggtttat cagaaaaatt
     6121 ataataacaa tcaataacta ccaaatctaa ttgccttcta tacgcaataa cattgtcact
     6181 ttctatatca ataggttcta ttgaaccata aatcaaacaa tcagaatact cactatcttg
     6241 tctcaacttt atcatctttt tataaaattc taaaatactt tctgaattgt taatctgact
     6301 ctgtgcatta atatctttat aattttcaat aacctttaac catggtgttg tatctgaaaa
     6361 acctgcattt tctttatctg accatggaaa tggcgttctt gcattatctc gacttcttaa
     6421 attcacaaat tctaacgctt cttcttctga gtaaccttca tctattgaac gatagtattg
     6481 gtcaatgcta gataaatcat taaaatcatt tatggtattt cttttaaaat ttgtcattcc
     6541 taattcctgt ccctgataaa taaatggtgt tcctcttaaa aagaaataca ttgctcccaa
     6601 catttttact gcatcatcat tcctttcgta ttgcaaaaga tactttgttg tagctcttgg
     6661 ttggtcatga ttttcaataa aattagctgc ccatccatgt tgctgaacat acatttgact
     6721 atctaaaatg tattttctta aatctgaaat tgtccatgaa attcttttaa accactcact
     6781 accagaagca acatctaagt cagcatattt aaagtcaaat accattgaaa aaaaaccatc
     6841 ttctccaata aattcaccta gctggtcata gctaatacct gctgcttcag caacagtcat
     6901 acaatcatat tttttaaacg tattttcttt taattcataa agcatttttt ccattccagg
     6961 ctgatttcta actgcttttg tacatttaac aagaccatca acaccatcac tttctaaact
     7021 ttgaaaactt aaattctttt tgatgaatgt aatagcatca actctaaatc ctgccactcc
     7081 catttgagac caggtattaa tcatttggta aagttcactt cttaatattg gattttccca
     7141 attaaggtca ggttgttttt ttccaaaaga atgaaaataa tattcgtctc tattctctac
     7201 cttttcccaa acacttccgc caaaaacaga cctccagtta tttggcaaat cgtttccttt
     7261 tttaaaaata taataatcat gataaggact ttctgggttt tttaatgctt cttgaaacca
     7321 aggatgttca tcactcgtat gattaagaac taaatctaat ataatcttaa ttcctctatc
     7381 tttactttct ttaattaact cttttaaatc ttccatactt ccaaattcac ttgcaatatc
     7441 caaataatca ctaatatcat acccattatc gtccataggt gatttaaaaa ttggacataa
     7501 ccacaatgat gtaataccta aatcttttaa ataatctaaa cgagaaacaa ttccctgtaa
     7561 atctccaatt ccatcattat tactatcttg aaagcttctt ggataaattt gataaacgat
     7621 ttctttttgc caccatttct tttccataaa ttactccttt ttatatgata tttcttcttt
     7681 ttaattcttt ataaattcca ttatcgaata tgtcctcaca tatagatgtt gctatatctt
     7741 taagctgttg atgactattt ttcattgcaa ttgtaatatc tgatgcttga aacatgacga
     7801 tatcattttg accatcacca aaacatatgg tttctttttg agttatccct aatctttctt
     7861 gtaatctttg aattgctttg cccttatgaa aatctttttg aataatttca taatactgac
     7921 tagaaatatc cctctgcgct agttccattt tatcttgtag tatatctttc acctcatcaa
     7981 atactttttc atttgaccat aaacaaattt tatgtatatc ttgtaactta tactcttcaa
     8041 tgttattttc atatgttatt ttttcttgga tatgttgttt atttatacaa gaattagttc
     8101 ctttcaactg attcattgtt tcaaatattt ctttggcttt ttgattcatg aaaacttttt
     8161 cctgactctc aatcgaaaaa gcaacttctc tcttttttaa taaacaaaca acttccttta
     8221 tcaatctttg attaaaagat tgattataaa gtaattcacc atgatattga atgtaattac
     8281 ctcctcctgc aatatatcca tcaaccccta atgataaaac atcatcttgt attgtaccca
     8341 tacttcttcc agtacaaata acaacactgc aatgattctt ttgacataaa cgtatagcat
     8401 gctttgcaga ttctgggatt ccatatacct catctctaag cgtaccatct atatcaagta
     8461 ttaataattt gtacaatttc atcttccttt cttactatca aaatcataat agcattcaaa
     8521 aaaaaagata tcaatatttt gatatctttt aaaaaatatg ttcattttta gcacaaattt
     8581 tcattttcgg ggaatatgtt cactttctaa atacatcttg tatttaatat aaagcagttc
     8641 aattaaaata aaataaggtg ttgttacatc gtagtccagt tgtttatcta cagaaataga
     8701 tatagattga atatataggt tttcatcaca taatcttgct aaaggattac ttatcatttt
     8761 tgtaatagaa accatataaa ttcctttcat tttcattttt ttagcaaagt caataattgt
     8821 agatgcatct ccatttaatg aaataaagaa aatcatatca tccgttttta taaaatgatt
     8881 aagtgcctga atcatatcat agccataaac atcatatata attttccctg ttggtaagaa
     8941 aatccttttc atctctttag caactctttc ctgagaataa ccttctccaa agacgattat
     9001 acgatttgcc tttttcattc tttcaaacat aatcgaacaa tccttcttct ctatatcatc
     9061 tataacctta ttataacaat ccattaattg gtgtttactt tgatttttat attctacttc
     9121 tgtttcattt aagcgtataa taactcttaa ttcactatat ccaggtaatt gtagtttttg
     9181 agcaaaacga gaaagtgcac ttgtagatat atggtatttg tctgcaaatt catctataga
     9241 aagtttaata caatcttctt tatgattaag aatacactca cagatatact tatcatttgc
     9301 tgtaaatgaa ttataatatt tattgaaaag tccttcaatt ttcattcttt tctccttcta
     9361 tactctatgt aattcatatg aagcaaatct aatagagcat aaaatgctcc tacgacttca
     9421 taagatatag cttgtaatgt atcaagtttt tgcgtttcaa catacagatt atactcacaa
     9481 agtgttgcaa ttgtattatt atcaagccta gttaaagaaa tcgtatgtat atgattgttt
     9541 ataaattttg caatttcaat tcctgctttc gtttctccag acaaagaaat gataataaat
     9601 aaatcacttt tttgaaatga ttctctcata aattcaattt cgcctatatc aaataagtca
     9661 ataacacatt tacctactga aagaaatatt ctctttattt cttgagctaa acttttctgt
     9721 tcattacctg tcccataaat ataaattgtt tctgcataat atattatttt acaaatatct
     9781 tgaaatgata ttttttgtaa ttcattcatt aatagtcgat aattctcgca aacatcatga
     9841 aaattattat cattattgac aaattctagt tgattatttt taagtaaata ctttaaatca
     9901 gagaatgaat ctaatccaat ttttctacaa agccttaata aagtagctct agaaacatta
     9961 cattcatttg caattttttc acacgaaaaa ttacggtatt ctctttgaca aatcaatatt
    10021 tgtttaataa taattttatc attttcattt aaattatcat aattttggtt aactaacgat
    10081 tctaatctca ttttttccac tccctaaata ttattttaat atacattagt tgctttttct
    10141 agggaagagt cttaaaaata tttctattaa gacaaataaa aaagagctat ctcagaacaa
    10201 atttaatttg ctcataccac agttctatta taaataaaaa aattttcaca ttatttttta
    10261 cactatataa tttctatttt tataattaaa cccaatttat aatatataga accatattta
    10321 tatttttatc tcctgatttg ttttaattac atctattcat tttctatctt atttaataat
    10381 tcctattact tgaatattta cttctttgag taaatttcat agctaacaaa gctaaaatta
    10441 ttattataaa tgtcaaaata caaatataat tcatattaaa aaagcttcca tctctttctt
    10501 ctatataagt cacaaaacta ataaaccatt gtgttgggct tataatacta attttttgta
    10561 acattgttgg cattttttca actgaaataa atgaaccacc aataagtgat aatataagtg
    10621 atatacatgt agaaaacaaa tttgcatgca tttctttatt gaataaagaa gatacaaatg
    10681 ttccaaaaga agttgccaaa gctgatatcc atactcccat taaaattaaa tctatataat
    10741 ccataccaat atcaatatta aataacttaa ttatagatat tgaaactatg tactgaaaat
    10801 acgttattgt aaagttgtat aacatattac ctagcacata tcctcttata ttttgtggag
    10861 aatatagcac tcttgtaaat gtacctgagt ttttatcttt aataatcatt gatgctgtta
    10921 cagtagatgt tatcattaaa aataatacga taaatgcaat tattctttgt aaaactccta
    10981 aagtattttc ttgtaattta cttatatcta caggaactgg gtctttttta tatgtttcta
    11041 ttaaatactt taatgtatca ttcgtatttt tatcttttat agaatataac tcaaaaccat
    11101 tcttattaaa acatatgaca gctgaatact tgcctgttat aatatcagtt ttaattgttt
    11161 taaaatctgc aatctctgta ttaatccctt gagtattttc taaagccatt attgtatttt
    11221 cataggttct tttttcagat tcctttagaa tgcattcgtt tttgtactca tctaaaatac
    11281 caatattaaa agatggttta cttatgtaat ttgccaatac tcctaaaact actataatta
    11341 taggaaataa aagtgtaact aaaaacacta ttctcttatt tatatttctt tttaagttac
    11401 tattaaaaac atttattaca ttcactatat aaagacctcc tttttaaaaa atttgattgt
    11461 aagtattgtc ataaatatgc ttacagctac aaatatgcat gatatattaa aaaggagttg
    11521 taaattatta tcatagatac aagcaactat gcctctatta atccatgtta atggtgatat
    11581 attaatgatt aatgaaataa ctggattcaa tgaactaact ggaaagaaca ctcctcctaa
    11641 gaagccaaaa ataactatcg gtatattaat gatattacgt aacatatcta aatttttaca
    11701 aacaagaccc aaaaacaacc ccatagatac tactgcaata gattcacaca ttaaaaagaa
    11761 aataacaact aaaaatttcc caccaaaact catatgaaaa attcctctta acaccactaa
    11821 agttatcaaa ttgtatatcg aaaatactgt tgtgcaagaa aaaagttttg aaataactaa
    11881 atctactatt gaaataggag aaatcatata acgatatgat gtgttagaaa ttttttcatc
    11941 ttgtgctaca tatgaaacac taataattcc cataagaaca caaaatggta ttgtaacaat
    12001 agtataataa ttatatgatg taaattcctt accataagac tttgatgtca aatatcctaa
    12061 tagtactatg attattactg gaaaaacaat attgtaaaac agaataaaaa aatctttact
    12121 tcttcgtttt attcctaatt taaacaatgt taaaaaacat ctcatctatt tattcctcca
    12181 aatctctcaa atctgtacct gtaagatata gaaaaatatc ttctaatgtc ttacaaccaa
    12241 attttaaata tttattttta tataaactct tgtctaaatc ttctataatt actcctttat
    12301 ctattaaaac aattctatta cataaagttt caatttcctc catatagtgg ctagtatata
    12361 tgactgttgt gccattatta ttaatgcctt taattgcttc caaaatttta tttcttgatt
    12421 gagggtctat acctactgtt ggctcatcca tgatgagaat ctgtggacta tgtgcaattg
    12481 cacaagctat gtttagtcta cgtttcattc caccagaaaa ttttgatgga aactccttat
    12541 gactttccca aagaccaaca aattccaagg catatttagt tctctcttta agttcattcc
    12601 ctttaaatcc atataatgag caaaaaaatt taacattatc atatgcactt aaatcttcat
    12661 atattgctaa ttcttgtggt acaactccaa gtgagctttt aaaaatattt tgttgattct
    12721 taatatcttt tcctttatag tatatgcttc cactatctgg agacacaatc gtagtaagca
    12781 tattgataga tgtgcttttc cctgctccat ttggtccaat aaaacctaaa atatctcctt
    12841 ccaataactt aaaactttct cttgatacaa cttccttatt gttatacttc ttagataaat
    12901 ttttaatctc taacaataca ttactcattt ataaaatcct cctaatattt aaccaacatt
    12961 atatatccaa tattgtatat atagtttttt taaaacacga gtgttttact cgtgttatta
    13021 taataaaatt tattacttat tttgtaattt cagtattatc ttatctacat tttctatagc
    13081 tttatctgga ttattaatta tttcatctct aagtctttga acttctaata actcatttgt
    13141 cgtatcagat gtaaataaaa aatcttcttc tatatttgaa aagtctatag atttaattat
    13201 attctgtgtc tcgcaaccaa cagatatata cttatcgata ttatttaata tttcttcatg
    13261 ccacaaaatt tgataattca gattatctat agtcatatca aatgcaattt tttctgttgc
    13321 aagactcttt ttatcaattt ttttaatctc tttccaattt tcccaatact tcttctgctc
    13381 tataagatat tttatatgtt tttcaagatt tttttgcaaa gtatccttag gtaatacatc
    13441 caaaatatta tgtaatagga atttatttga accagttgga actattggca tctgaagctc
    13501 tttttgtatt tcttccctta attctaattt accgctttga gtaatctcat agattttacg
    13561 aatcctagct cctgtttttt cttcttttaa aactctaact cctccatcct tttctaattt
    13621 tgccaatgca taatatattg acccagattg tattttagtc catctatctg caccagatac
    13681 ttgtaaaaac ttttgaatct catacccatg tgttggctta atatttagat aatataaaat
    13741 taaagtgcgt atcataaaaa ttcaccacct atactctatc ttggatatta tattatcacc
    13801 aacattggat atagtcaata gataacatag gatatattac aacttattat ttttattatt
    13861 taatacaagg agttccatga gacatacata tccattcata ttttaaatta tccaattctc
    13921 ttatagattt catcatcttt ttataatccc aattccatgg attaggataa ggaactatat
    13981 ttccattctt atttttgact aaatcacctg caaataatac atcttcaaaa atcatgcaga
    14041 catgccctgg agtatgacca ggagtatgga taatttttat attcccaatc tgcatattct
    14101 ctccataagg ttcaacatcc ttaatgagcc ttctggaaat tacctttcca ataaacttct
    14161 taaacccagg tctatccttc tctcctataa taaaaggtat atcctctaca tgagcccata
    14221 cttttgctcc tgttttttct ttcaataatt taatattaga tatatggtca acatcatgat
    14281 gagttaataa aatatgctta atatctgtaa gtttaatacc aagtgttttt aattctttaa
    14341 ttatattttc ccccataaaa ggaagacctg tgtctataag aataatttct ttatcaaaca
    14401 caataaaaca atttgagcca cgagtactat ctaacattaa tacattttct tttatcttca
    14461 taaataaatt ccccttattt ggtattttac ctatataatt aattaataaa gttataaata
    14521 gttataatac ttgatattaa ttttattttt tattaaagta tactataggt atacaagtat
    14581 tatatttaca taagtatacc atcagtatac ttttttacca atagaaagga ataatttata
    14641 tgtcagaatt atcaaaaaga gataaagaaa aaatacaaag agaaaatgag attattgata
    14701 aggcagaaaa attattttgt cttaatgga
//
LOCUS       DAEORZ010000363        11140 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.guided.138, whole genome shotgun sequence.
ACCESSION   DAEORZ010000363 DAEORZ010000000
VERSION     DAEORZ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.guided.138"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            111..617
                     /locus_tag="KRH39_003928"
     CDS             111..617
                     /locus_tag="KRH39_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513784.1"
                     /translation="MKISSQYRSQYSFRHESNVNNTRINESIVKKNEIVGKDTYLSNI
                     MKQKQELNDRIRDLKDRQEIYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNRIIED
                     KNKSTMLDENSTYLNTNDEKESLIAKEFNEKVEEEILNDEKLEELEQKRIIKRILIKK
                     RRYLRTYP"
     gene            647..808
                     /locus_tag="KRH39_003929"
     CDS             647..808
                     /locus_tag="KRH39_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513785.1"
                     /translation="MLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIFDKTDE
                     QKKHINVLV"
     gene            852..1400
                     /locus_tag="KRH39_003930"
     CDS             852..1400
                     /locus_tag="KRH39_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513786.1"
                     /translation="MEGLGFYAAALAGGSYQRIGFGKLNPIEVIADGDWISYKQAQDT
                     LTVIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLLDKRGVCGSFA
                     SSFHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGTLDFDYCKLK
                     DATREMTTYYDAKALSILGFNQ"
     gene            complement(1712..2668)
                     /locus_tag="KRH39_003931"
     CDS             complement(1712..2668)
                     /locus_tag="KRH39_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG8513787.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRITDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSKILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3371..4366
                     /locus_tag="KRH39_003932"
     CDS             3371..4366
                     /locus_tag="KRH39_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8513788.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            4344..5207
                     /locus_tag="KRH39_003933"
     CDS             4344..5207
                     /locus_tag="KRH39_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG8513789.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTIIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            5204..5998
                     /locus_tag="KRH39_003934"
     CDS             5204..5998
                     /locus_tag="KRH39_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG8513790.1"
                     /translation="MIKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            6283..7662
                     /locus_tag="KRH39_003935"
     CDS             6283..7662
                     /locus_tag="KRH39_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8513791.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIC
                     KYKNRYKYM"
     gene            8112..8825
                     /locus_tag="KRH39_003936"
     CDS             8112..8825
                     /locus_tag="KRH39_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8513792.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLTNDGFKNIIKAYNGLDAI
                     SLCKITCPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIVFRVKAQLRRQQYQSIVPSDSEVIKVGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(9103..10641)
                     /locus_tag="KRH39_003937"
     CDS             complement(9103..10641)
                     /locus_tag="KRH39_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG8513793.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGAGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINKDTTISLYLHTAFEKLYKIEKEMN
                     LLYQKSAISGNEQHLIKASDYQEQLIANNFYYIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSDYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEEIFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         4043 a         1266 c         1678 g         4153 t
ORIGIN      
        1 ctataaaact attaactaat atttgtaaat gtatactttt gatgatagaa tataattaag
       61 ttaagcttgt tttttataaa acataaattt attttagaga ggtaaaataa atgaaaatat
      121 ctagtcaata tagaagtcaa tattcattta gacatgaaag taatgtaaat aatacaagaa
      181 taaatgaaag tatagttaag aaaaatgaaa ttgtaggaaa agacacttat ttatctaata
      241 ttatgaaaca aaagcaagaa cttaatgata gaattagaga tttaaaagat agacaagaga
      301 tttatactaa aaaaataaat gaagcaatta agaacttata taaatcagaa ataagagaaa
      361 caactaataa cttttctaat atagaaatag gtattaaaaa tagaattata gaagataaaa
      421 ataaaagtac aatgttagat gaaaattcaa cttatctaaa tacaaatgat gaaaaagaat
      481 ctttaatcgc taaagaattt aatgaaaaag ttgaagaaga aatattaaat gatgaaaaat
      541 tagaagagtt agaacaaaaa aggattataa agaggattct aataaaaaag agacggtatc
      601 tgaggactta tccttagtag gtaaaactcg tgaagagctt gaaaatatgc ttaaaaattt
      661 tataaattta acacaagaag aaataatgaa acttgagtcg agaatagaaa agttagataa
      721 agatgctgaa gaatacaaac aaaattcaaa gactaatata tttgataaaa cagatgaaca
      781 aaaaaaacat ataaatgtac tggtttaaac aatatgtttt aaaaaaataa gtttattata
      841 taaataaggg tatagaaggt ctaggatttt atgcggcagc attagcgggt ggctcatatc
      901 agagaatagg ctttggaaaa cttaacccta tagaagtaat agccgatgga gattggattt
      961 cttacaaaca agctcaagat acacttacag taatcaggaa ttttttaaat agttttgact
     1021 ggagaaatgc atctgagatg gaaagagcta atagagctgc aaaattggta accgaagcaa
     1081 aatatgcaga ctctaaatat tgtaacatag tctatggtaa tctgcttgat aaacgtggtg
     1141 tatgtggaag ttttgcaagt agctttcatt tattaacaag gttaatggga atggattcac
     1201 tatctatatt aaatccatca ttaaatcatg cttggaacta tatacaaatt gatgggaaat
     1261 ggtataggtt tgatgggtct gaaatatcag cttttggtgg aacattagac tttgattact
     1321 gtaaattaaa ggatgcaacg agagagatga ctacttacta tgatgctaag gcattgtcta
     1381 tattaggatt taatcaataa aagcaaaaaa ggctctttat aaagcctttt taagtgtttt
     1441 ttaagtgaaa ttttttatat cacatcagtt ttaatcaaaa aatataacat tttggactta
     1501 cttttaaaaa taaaattatt aaaatataat attaatcatg ttaataatgt ttttagttta
     1561 ttatataata gtggttatgt tgactattgt agttttatta agaatagttg gtttatataa
     1621 atatatgcaa agataacttg ttgtttactt tatttattag tatatagtaa aagatactct
     1681 aataaatttg cttattttaa tatcttttgc actaaaacca actaaagttt cctttattat
     1741 ttcgttttga ataatacaaa gcataatccg attgtttgta taaagactgg aaagtatcag
     1801 agttattttc taatttagat atacctatag aacaagaatt actctctagg cataattcta
     1861 aaactttctt tgctttattt tcaacattct cttttgattg attactatat tcaaaaacag
     1921 caaattcatc tccaccaagt cttccaataa tagcatcatt tacgaagact ttagtcaaaa
     1981 tctttgaaaa atgtattagt atttcatctc ctcgaacatg accaaagata tcatttacct
     2041 gtttaaaatt atctaaatca aagatgtaaa agtatccatg tgaaacagtt ttaagcattt
     2101 cttttatgtg tacttcaaaa aaatgtctat tgtatagtcc agtcataagg tctttttctg
     2161 attttagttt aaattcattt gccattgcta gtgcttcttg tgctttttca gttattgttt
     2221 tagggtaata ctcatctttc atttggtcaa agtttggaat agaatggtga acatggataa
     2281 gtttaatcat atcatctgta attctgtata ccatagtaaa tcgagtatcc atttctacat
     2341 agatatcttt ttctaggtaa tctttttcta ttacccaaag agtaccatat actataaaag
     2401 cttcgtctgt aactttggta catgaatacc actcatctat aatctcaaag tctatttgct
     2461 ctgattctgt ttggtctttt tcaagaccta taataaattc ttctaatgtt tcgtaaaatt
     2521 catgttttcc tgttccaatt aatgcaaggt tgtcatctaa taaggaaata ataaatttaa
     2581 ggttttcttc ttctggattt aaaatataaa tatgccaaat tttttttgat aaatccctta
     2641 attgtttttg tattgtatta ttttccatag attaccccct taagatttac agtttatatt
     2701 ttaatgtatt ataagttgaa ctcttattac aatattatta taatatagaa ctaaagaaat
     2761 tacatcctgt ataggtaaaa gtatatcttt tacaataaaa atattacagt aaaataactc
     2821 gttttaatat acatattata tttttaattt ataaatgaat attataaatg aaatttaaat
     2881 tcaaccctga agaaataagc taaatgtatt agtatataaa tccatacttg aacataaata
     2941 acctttatga tatgatatca ataataatat tatgagagga gtgccttgat gtcggagtta
     3001 tataatggta accaacaatt aaatagttgg agacacatat attctgtata atcagaaatt
     3061 ttaagtgtat ttttaataca tttaattttt gtgtcttatt ctgcctttat agataactga
     3121 catatgacaa caaatagtat ttaaaatata tattactttg ttaaattatg gtgtagtagg
     3181 ctttaatctg ctacactttt attaattttg tatagtatta ttttgataac tattttgaaa
     3241 tagagagcat taatttttct agtccacagg agttttctgt ttttgtgctg ttaaatataa
     3301 gcacagaaaa aggcagatat atacctgtgg acttttttta tataaattat gaaatatgga
     3361 ggtagttaaa atgagtataa ttgaacttaa tgaactgact aaatcttata gaagatttga
     3421 gaaaaaggca ggatttatgg ggagtgtaaa gtctttattt aataggaaat atacagaaaa
     3481 aacagctata tcgtcatttg atttaaagat agaagaagga gaatttgttg gattaatagg
     3541 tcaaaatgga gctggcaaaa ctactcttat taagatgtta actggtatta ttgcaccgtc
     3601 ttctggagaa atatctgtaa tgggttatta ccctaacaaa ttagaaaata attttaaaaa
     3661 gcaatatgct gttgttatgg gccaaaaaag tcaactattc tttgaattga caccagcaga
     3721 tacgtttctt ttatttaaag aattatatga tatatctaat gaagaatata aaaaaaactt
     3781 ggattacttt atcgaattat ttgaggtatc tgactattta aatgttcaag ttaggacgct
     3841 ttctttagga gaaagaatga aaatggaact aatagtagcc cttctacata atccaaaaat
     3901 attatttttg gatgaaccaa ctataggctt agatgctata gcccaaaaac aaattaggta
     3961 tttcctaaaa gaagtaaatg aaacaaaagg aactactatt atacttactt ctcattacat
     4021 ggaagatatt aagagtctct gtagtagaag tattgttgta gaaaatagcc gtaaaatata
     4081 tgatggttct acagagaaac tatttaatca ttttcaaaaa tataaaaaaa taacagttag
     4141 tttttccaat gaaactaaca ttgtatttcc aaaagattgt aaagttgttg aacaaacaaa
     4201 tcataagata tcagtaattg tgccaaaaga aagaggaaat gaaacgatta aatggatttt
     4261 tgataaatat gatgttaaag atgtaatgat tgaagaagaa gatataggta gtattgtaga
     4321 gaggatatat aagaaaggaa gtgatgagta ttatgagcaa ctataatgaa gcaagttcaa
     4381 atgattcaat gttttataaa tttaaacaga gtattagaag gtattatgaa gtagccaaaa
     4441 ttaactttaa agtgcaactt atctggagat ttgatgtggt ggttaatatg gtgtttacga
     4501 ttgtaaaaat tttatttgct tatattttat ggaaagcaat ttttggtgaa aataaaatag
     4561 tctctggttt tacatttaac tctatgttgt cttactatat aataagctca tttttagcag
     4621 gtattgatat gtcaaagagt accagtgaag agatatgtaa tcgtgtaaaa ttgggcacct
     4681 tttcaaagta catgattatt ccaattaatg ttcaaaatta ttttattgct aaaaattttg
     4741 gaatgacatg tttttatttt gtatttaata tgatttctgc atttgtatgg atttttatat
     4801 ttaaaataga ttttgccttt acatcaaatc cagttcaaat attaatttca attttgatga
     4861 tttttctagg gtttattttt atgatgaaac tccacttctt tattgggatt ttgtcattta
     4921 aatttcttga gattagtttt tttagaatga taattgaaaa tattgttgaa tttattacag
     4981 gaacaataat accacttgta ctcttaccag aaactatagt aacagttatg aaggtatttc
     5041 cattttatta tacaacatat ttgccatcta tgttactaat aggtagaaac agcaatgagg
     5101 caatacaagg attaattatt ctagcattat gggttttatt tttttctgta gtaaataaag
     5161 ttatgtataa aagaatgaga acattatttg atggggtggg tatatgatta aaaaaaattt
     5221 gaaaataata tttatgttat taaaaatgaa attatcaaag atgatggtat ttagatttga
     5281 tttttttgga gcattttttg tagatagttc attatttata ttacaacttc taatgtttaa
     5341 tagtatatat tctcatgtag actcaatagg gggatggcaa caaggagaga tgttaatatt
     5401 tataggaact ttctcattaa taaatgcgat aaatatgacg attttctttt ttggtattta
     5461 tgatattcca cgaaaaatcc aggaaggaga attagactac tatattacaa aaccattaga
     5521 cccactattt aggttaacat ttgaaaatat taatattggt tcatcaccat taattctagc
     5581 aagtatactt attgttttat atggaatatc tgagttaaac tcagagataa ctttagttat
     5641 gataataata tatattttca tggtgttatt gatgacatta cttttttatg atgtatgtat
     5701 catacttaga acagtttcat tttttgttat atcatcttct gcgataatga gattagaaga
     5761 taatttattg ccaatgaaca tgaaaattcc tggtgtaata tatgaaggtg tttttaaagt
     5821 attattctat ctaatcttac catatggaat tatgtcgaca attccgactc aaattttggc
     5881 tgggacaata acaattaatg gattaatata ctcaatttta attgtatttt tgtttacatt
     5941 gtttatgtgg agattttgga agttaggtat gagacattat aaaagtgcaa gtagttaata
     6001 aattggtaaa aatagaaaat gtataattat atataaatac gtttcatatg acactaaact
     6061 atgtaaacaa gtatttgcac taaaggcact gaaagctata aaatgattaa agtaaggatt
     6121 taaaagttac tttagtagaa atataattgt gtaaaaaagc aagattaatt aaaactatat
     6181 ccgaataatt agatataatt tttatgcaga aaggaggtct gtaaatgtct aattgtttgg
     6241 aatatatttt aaaaataatt aatgtacaga aaaataaaca aaatgagtga tgtatattat
     6301 tcagaaaatc atgaacaaga taatctttat gagcaagata atttactggc acaaagattt
     6361 aatttattta ctctattgaa gtttgcattt cctactattt ttatgatgat ttttatgggg
     6421 ttgtatacaa tcgtcgatac tatatttgta tcaagattta ttaatacaaa tgctctatca
     6481 gcaattaata ttgtatgtcc aattattaat cttatagttg gaattggaac tatgcttgcc
     6541 actggaggaa gtgctattgt agcacgtaag atgggaaatg gtgatattga gggagcaaat
     6601 aaagctttct ctttaatcgt attatcagga gttgtaattg gagtaataat tactggcata
     6661 ggtattgtat ttatggatta tatcattata tggttaggag caagtgaagt tttaattaaa
     6721 tattgtcgtt catatctttt tattatcttg gtttttgctt ctgcgaatat actacaagta
     6781 ttatttcaat gtttatttgt aacagctgga aagcctcaat ttggattatg gctagtgtta
     6841 ggtgctgggt gtgctaatat aattcttgac tatgtgttca ttgttctgtt acaaatgggt
     6901 attagtggag cagcacttgc aactggagta ggatacctaa ttcctaccat ttcaggtatg
     6961 gttttctttt caaaaaataa agggttactt aaattttgta tacccagact agataaaaga
     7021 atattatttg aagcttgttt taatggttca tctgaaatgg tgggtcaact atcaacagca
     7081 gttactactt ttttatttaa tgctatgatg atgaaattaa ttggtgaaga tggagttgca
     7141 gctattacaa taattattta ttctcaattt cttttaactt cgctttatat aggtttctca
     7201 atgggtgttg caccaataat aagttataac tatggaagtg aaaacaatac gcagttaaat
     7261 catgttttta agatttgtat attatttatt tcagtagcat ctttattggt atttatcttt
     7321 tctatatcat gttcaccaat cattgtagag attttttctc aaagaggaag ccgagtttat
     7381 gaaattgcaa ggtctggatt ttatatattt tcttttagtt ttattttttg tgggtataat
     7441 atttttactt cgtctatgtt tacagcatta tcaaatggaa aggtgtctgc aatattatct
     7501 tttctacgta catttggatt tataactata ggtttattga tattgccaaa tatattaaat
     7561 ataaatggga tatggttagc agtaccaagt gctgagattt taactttatt tttaaccgtt
     7621 ttttgtatat gtaagtataa gaatagatat aaatatatgt agatattaaa taaatagttt
     7681 aggtagatat tttatgtttt aataagtaat tcaattagtg gtagatagat actattgaaa
     7741 attaaagtta taattgatat aaagaattaa taaaaataac atataaaagt ttaaaagggg
     7801 agttgaactt aaagtttgat tagaaaaagt caattttaag acaactcctt ttattttact
     7861 attgtattaa tctgtatctg taagtgtatt agtctaatat tataaacttt ggatgaaatt
     7921 aattttctat aggttttaat gtatcataca ctttatttta aagaaactca tcttaatttt
     7981 cataatgatt gaatttgtat attagagatg ttactattag taatgtaaaa gcaagagtag
     8041 tgaatataaa aaaacaagat agtgatactg caagattaga atataataaa tttttatgga
     8101 gagatttcaa tatggataca tattttaata aaaaaatatt acttgttgat gatgaaaaag
     8161 atatagtgga tttaattgaa gaagtactta caaatgatgg ttttaaaaac attattaaag
     8221 cttataatgg tttagatgct atttcgctgt gtaagataac atgtccagat gttgtcattc
     8281 ttgatataat gttaccagat atagatggta tagaagtgtg taaaaaaatt cgagagtttt
     8341 catactgttc tattcttttt ctctcatcaa aaaatgatga tattgacaaa atactaggtc
     8401 ttagtagtgg gggagatgac tatataacaa agccattcag cccaagggaa attgtttttc
     8461 gagttaaagc acaattacgt agacaacagt atcaaagtat tgtaccatca gatagtgaag
     8521 ttattaaggt aggagatatt actattgata tagaaggaaa ccgtgtatat aaagatagaa
     8581 atgagattga gttaactgga agggagtatc atcttttaag ctacatggct caaaatgtaa
     8641 ataaaattat aggaaaagaa agactctatg aacaagtatg gggagtatat agtagtattt
     8701 gtgacaatac aattatggtg catatacgac atataagaga aaaaattgaa gataacccct
     8761 caaatcctaa aatacttata acggttaaag gactaggata taaacttgtg aatagaatcg
     8821 attagaatag attttaatat caattaagat gtctcaaatt gaatttttta gttaatgagg
     8881 cattctttta tgtgtgaaat caaatgtatt ttcgttatct caataatgaa gaagaggtct
     8941 atctctattt tgagatagcc tcattaagtt tatatcctgg taatgtactt tatgttctaa
     9001 taaatattta tttttttagt ttatttgcag tgaaacatta actagaacat taaaatttgc
     9061 attgaaatac caattaaata atattgtata ttgtgtataa acttatacta attgtttttc
     9121 tatattaaat actttatcaa tccaaccttt ataaaatttt tcttcatggg aaactaggat
     9181 tatacttcct ctaaactgtt tcaaagcatt ttgtaaagca tcctttgtct ctgcatcaaa
     9241 gtgattggtt ggttcatcta gaataagaaa attacaagga gataacatca tacaacataa
     9301 tttaacctta gattgttcac caccacttaa agtagataca cttcttgaaa catgctcttc
     9361 cttcacacca catctggcta gatgacgtct aatctctttt gtatttaatt ttggatattt
     9421 atctgctact atttgaagtg gggttttatc aggattttcc cattttaaat cttgctcata
     9481 ataaccaatt ttaacttgtt ctgaaaactg gaaatctcca gatatacgag gaatatcttt
     9541 gactaatgtc tttaataagg tagatttacc gattccatta aaaccagtta taactatttt
     9601 ttgacctcca tcaacagaaa aatttaattt tggaagtaaa gaatagtagt atccaacttc
     9661 aagatttgtt atagtaagag cattttgagc cgatatctca atttctgaaa attgaatatt
     9721 tggttttcct gtaaaactag gaggagctat tctttctatt ttatctaatt gtttttgtct
     9781 tcctcttgca atttttgaat ttactccagc tttattttta cgtataaata tctcttcttt
     9841 ttctattttc ttttgttgtg cttgatatct tctaatataa tcttctctta aatgtgattt
     9901 ttgtttaagg aactctgaat attttccatg atatttctta atcatgccaa attctatatc
     9961 aagaatacct gtagaaattt tctctaaaaa gtcaaaatcg tgggaaacta caataaatgc
    10021 tccattaaat gtatttaaat aatctgagag ccattcaaca tgttctttat ctaaaaagtt
    10081 tgttggttcg tctaataata aaacatcatg gttagaaagt agtaattttg ctaaaattac
    10141 ctttgccctt tgcccaccac taagctctcc aacaacacga ttcattccta ttgaatctaa
    10201 tccaagacca tttgctattt tatttatttc attatctata taataaaaat tatttgctat
    10261 tagttgttcc tgataatctg atgctttgat tagatgttgc tcatttccag atatagcact
    10321 tttttgatag agtaaattca tttccttttc tattttataa agtttttcaa atgcagtgtg
    10381 taaatagagt gatattgttg tatctttatt gatttctgca tattggtcta aatgtcctat
    10441 attgatatta ggttgccact tgatttctcc tgaatctgga acaacttctc ccaataaaat
    10501 tttaattaat gtacttttgc ctgcaccatt ttgaccaact acacctatat gttcaccttt
    10561 gaataaatca aagttagctt tctcatacag tgatttatcc ataaaacatt gtgataaatt
    10621 agttactttt aataaactca taattcaaac tccttttctt ttattaaaaa atttatggaa
    10681 ataaaaaagc agaggaatgg cattgtaaaa tgccgacctc tgcttttgga aaaacaaaag
    10741 gtcatagaca agcattgttg tctatgacca gaaaatctat attatctatc ggcaaatagg
    10801 taattttaaa taagcatagc caaaaaacta tactaaacct atatttgccg atgaagatag
    10861 aaagctttaa gctttcagat taacctctac acaacgcttc atcgtataca tttgtgtaga
    10921 gttaagtttt ctaacaagtt taactaattc tatataaatc ataattattc acatcctaat
    10981 cttagtatta attctagttt atctatattt aaattgtact tttgtacttt gtggattatc
    11041 ttatttattt aaaatatgtt ttattaatta tagcttaagt tatgttatca taatacttag
    11101 ttatagtcaa taagaaagtt atatttacta tatattatat
//
LOCUS       DAEORZ010000364        10282 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.guided.144, whole genome shotgun sequence.
ACCESSION   DAEORZ010000364 DAEORZ010000000
VERSION     DAEORZ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.guided.144"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            247..864
                     /locus_tag="KRH39_003938"
     CDS             247..864
                     /locus_tag="KRH39_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG8513794.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            972..3503
                     /locus_tag="KRH39_003939"
     CDS             972..3503
                     /locus_tag="KRH39_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG8513795.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNIKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3937..4638
                     /locus_tag="KRH39_003940"
     CDS             3937..4638
                     /locus_tag="KRH39_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG8513796.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4688..5761
                     /locus_tag="KRH39_003941"
     CDS             4688..5761
                     /locus_tag="KRH39_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8513797.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLISKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIV"
     gene            6031..7131
                     /gene="vanG"
                     /locus_tag="KRH39_003942"
     CDS             6031..7131
                     /gene="vanG"
                     /locus_tag="KRH39_003942"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG8513798.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPILHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7128..7934
                     /locus_tag="KRH39_003943"
     CDS             7128..7934
                     /locus_tag="KRH39_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG8513799.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7952..10090
                     /gene="vanT"
                     /locus_tag="KRH39_003944"
     CDS             7952..10090
                     /gene="vanT"
                     /locus_tag="KRH39_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG8513800.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDMCGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT         3907 a         1075 c         1846 g         3454 t
ORIGIN      
        1 tcaaaatgaa ctttttagtt catgaggcac tctttttgta cgaaataaaa tatattttta
       61 ttatttcaac aatgaaaaag aggcttgttt ccattttgag acagtataat ttttatgtaa
      121 ctttaatttt agtaaatata aagttttaaa attgataaaa taggaggaat ttttaatata
      181 aaagggtaaa taaagggttt agagggcata atatagaata aattaatata tttaaggagg
      241 gtttttatgt gtaaaaaaac gtatttatta gtggtaagta tgcttatagc gggtttatta
      301 actgcatgta gtgggagtcc aactaaggat tcaggacaag cacaagataa tacaaaaaag
      361 gaggcaaatg catcaaataa cgctttaaaa gatgaaaaaa ataatgaaaa tttaatggaa
      421 caagatttta aagtgccata cacagatgca ataaacatat ttaaagataa atataaggat
      481 gctgatatag tagatttaag tcttgagaga gatttaaata aatttgttta tactgtggaa
      541 ggtgtagatg ataataatga atataagatg aaaattgatg ctaatacaaa agatgtatta
      601 gaagataaaa cagaaaagtt ggattcagaa gatttaaatg gagttgctag aaaagaaaaa
      661 ttagatttaa atgacataat gacacctcaa caagctatgg aaattgcatt aaaagaacaa
      721 aatggaatag ttaaagaatg gagtttagac aaagatttag atgttacttt ttataaaata
      781 agaatagata aagataaaaa tgaatatgac ataaaagtag attctaaaaa gggaactgta
      841 ttgaaggtag aaaaagaaga ttagaagatt taaactatct ttaaaataat ttcttttttg
      901 aataaatttt gtttaaaata taacaagcag ggtataaaga gactaaataa ctattttagg
      961 aggagtacgg tatgtcattg aaatcactaa aaataaaaga aaatttatat tgggtaggtt
     1021 cgcttgaccc tgatttaaga gtatttgaca taattatgta tacaccttat ggaactactt
     1081 ataattcata tgtattaaaa ggaacagaaa aaacagtttt atttgagaca gttaaagata
     1141 agcattttga caattatatt gaaagattaa atgatttaaa tatagatttt gaaaaaatag
     1201 actatattgt tgtaagtcat actgaaccag accatgcagg aagcgttgaa aagcttttgg
     1261 atttagctaa aaatgcaaaa gtagtagctt cagaaacagc aattaaatat cttaaggaaa
     1321 tcgtaaacaa agactttgaa tatgttgcag ttacagatgg agatacatta tcaattgggg
     1381 acaaaacttt agaatttttc tctgtaccta tgcttcattg gccagatacc atatacacat
     1441 atataaaaga ggataaaact cttgtaacat gtgattcatt tggtagtcac tatagtaatg
     1501 ataagatagt aaatacactt gatgaaaatg aagaaaagga ttacttggat gctttaagat
     1561 actattatga ttgtataatg ggtccattta aaccatctat ggtaactgct atagaaaaga
     1621 taaaagattt agatatagat actgtatgtc caggtcatgg tccagtatta actgaaaatc
     1681 ctagaaaaat tatagacctt tattacaact ggagtgtaaa tgaacaaata aagttagaaa
     1741 aagaagttac aatttgttat gtttctgcac atggatatac aaagattatg gcagaagcta
     1801 taaaagcata tattgaaaaa aatagcaact ataaagtaaa tttatttgat gtaatagagc
     1861 ataagcaaga agatattttg gcaaaaatag cagtatcaca aggtgtatta tttggaacac
     1921 caacaatatt aggagatgcg ttaaagccta tatgggatat attaatatcg ttaaacccag
     1981 ttcttcatgg tggaaaagtt gcttcagtat ttggttcata tggatggagt ggagaaggta
     2041 tagaaaatgc tatggagaga ataagtcagc ttagaatgac agcagtaaaa ccttttgctg
     2101 ttaactttaa accatcaaat gaagagattg ataaattaca ttcttataca ggtaaattcc
     2161 tagataagtt aaattctact tttggaagta aaaagaaaac taagaaattt aaatgtgtaa
     2221 tttgtaatga agtttttgaa ggtgatagtg ctcctagtgt atgccctgta tgtggagcta
     2281 aggaagacca atttatagaa gttgaagaag atgaagttac ttttagaaaa gatactgatg
     2341 aatatttcgt aatagtagga aatggagcag ctggttttta tgcagcagat gctataagaa
     2401 aaagaaataa aacttgtaag ataactatga tttctaatga agatgagttg acttactata
     2461 gacctgcact ttctgatggt ataaatgaag aacttggttc agatttctac atggaagata
     2521 aagattggta tgataaaaat aatatagtag taatattagg aacgaatgta gataaattag
     2581 atgaagtgaa taaaactatt atagtgaatg atggagctat taaatttgat aagcttgtaa
     2641 tagctacagg aagtagaaac tttataccac caattaaggg tcatgattta gaaaatgtgt
     2701 ttactcttag aaatataaaa gatttatata gtgtaaaaga agcactagaa aaatctaaga
     2761 aagttgtagt gattggtggc ggtttattgg gacttgaagc tgcatgggaa tttagactaa
     2821 aaggattaga agtagtagta gttgaagcca tggatagcat actatcaaaa caacttgata
     2881 aagaaggtag taaaatatta gaacaatgtg taagagatac aggaatagat gtaagattgg
     2941 gtgtagcagt tgatggtatt gaaggtgatg gaaaagctca aaaagttgta tttaaagatg
     3001 gagatagtgt tgactgtgat atggtagtat tttcaattgg tgtaagagca aacactcaaa
     3061 tggttcagga tacatctgta aaaattgata gaggtattgt tgtagataag acattacaga
     3121 caaatattaa agatatatat gcttgtggag atgttgcaca agtgggaaat attagtttag
     3181 caatatggcc atcttcagta gaaatgggaa aaatagcagg ggcaaatgca agtggagata
     3241 atttgacatt tgaaagtgaa gtatatccag tttcattaga tgcaatgaat gtaaaagtat
     3301 ttagtattgg taatatacaa aattttgata aagaaatttc atcaaaagat gaaggtcaaa
     3361 gaatatataa aaaattattt atgaaagatg gttcattagt tggagctata cttataaatg
     3421 atttaagctg tactgttaaa ttgataagat taatatctga aaaaggtgat tttgaggata
     3481 ttatgaaggc tgatatacta taatttaaat acattaaaat aaaaaggagt tgtctcaaaa
     3541 ttgatttttt tctaatcaaa ttttaaattc aactcctttt ttgaattttt atataaaata
     3601 acatggaaat tttagtaatg tctgtggtta catacatagg tttaattgtt ttttatatgg
     3661 attgtctctg gtataataaa ttgaattgtg tttgactgat agatattttt attgaaagtt
     3721 aatacctgtt atgcgataat aaggacgtgt taggggagtt tttataataa caattaatct
     3781 cagtttttgt gttccaatag tagcatcaac acattctgat gtagtagttc ttttcttata
     3841 aaaattagtt acaaatatgc ttgtaattat ttgatatgta aatttaccaa tattataaat
     3901 gatgacctcg ataataagga gagtgaaaaa taatctatga acgaaaatat tttgattgtg
     3961 gatgatgaaa aagaaatagc agatttaatt gaagtatatt taaaaaatga aggttttaca
     4021 gtatacaagt tttatacagg taaggaagct ctagaatgta ttgaatctaa aaatttggat
     4081 atggctattt tagatattat gcttcctgat attgatggat ttcatatttg ccagaaaatt
     4141 cgagaaaaat actattatcc tatcattatg ttaacagcta aaattgaaga tatagacaag
     4201 ataatgggtt taaccatagg tgctgatgat tatataacaa agccatttaa ccctttggaa
     4261 gttgttgcaa gagtaaagac acaattaaga agatatatgc gatataataa ttcttatgaa
     4321 caacagtcga taattgttaa tgagtatgat attaaaggat tgattatcaa taaagaaaca
     4381 cataaatgta gtttatttgg gaaagaagta gcattaacac cgattgaatt ttctattctt
     4441 tggtatttat gtgaacatca aggaaaagtg gttccttctg aagaattgtt tgaagctgta
     4501 tggggagaaa aatatcttga taataataac acagttatgg ctcatatagg gcgattacgt
     4561 gaaaaaattc atgaaccttc taaaaacccg aaatttataa aaactgtatg gggagtaggt
     4621 tatacaattg aaaagtaaaa atattaaagt caatcctgac tttactcaag tgagaaataa
     4681 aatttttatg agaacagctt taatggcatt tatagccatt attttcatat ggattttata
     4741 ttcttttata ttgtatggta agttttctat ctggatagta tcattctttg aaaatgcttt
     4801 tcgtatggaa tataaagctg cattgaattt atatcagcag attttccgta atagcatgga
     4861 gctcttcatg atactagcta tggtgattgt gttttttata atattccgtg tttatttaaa
     4921 ttggtttaca aaatatttta gtgaaataaa tcaaggtatt gactctctta tcaaagaaga
     4981 tgttggagaa gtggctttat ctccagagtt attggcaata gaaaagaaaa taaactcaat
     5041 taaacatata cttgaacaac gtaaatttga gacacaaatg gcagaacaac gtaagaatga
     5101 acttattgtt tatcttgcac atgatttaaa aacaccactt acatcagtta tagggtatct
     5161 gacactactt cgagatgaaa gtcagatttc tgaagaactg cgaaaaaaat atttatctat
     5221 ttcgttggat aaggcagagc gtcttgaaga tttaattaat gaattctttg aaattacaag
     5281 gtttaattta tcaaatatat cacttgaata cagtacagta agcttaactc gtatgcttga
     5341 acaactaaca tatgagttta aacctacttt gattagtaag aatttaaaat atacactgaa
     5401 tgttacacca aatatgatga taaggtgtga tgttaataaa atgcaacgtg tatttgataa
     5461 cttattacga aatgctgttt attatagttt tgaagatact actattgaaa taacagctgt
     5521 acaggaggat gattatataa agcttaaatt tatgaatcat agcaatacga ttccagagga
     5581 aaaacttgag cgtatttttg aacaatttta caggttggat acatctcgta attcaaacag
     5641 tggtagagct gggttgggac ttgctatagc taaagaaata gtggagcttc ataacgggac
     5701 aataacagct catagcgaga acaatataat tacctttaag gttattattc cgattgtatg
     5761 aaatttgtaa gaatttaagt ataaaaaaat aggaaattct ttaggataaa taaataaaga
     5821 gcactcttgt tattgatata attactgtat caaaacaaga gtgcttttat tttgatacag
     5881 taattatatc aatagaaaaa tgtgaaaata tattatgtgg aacagatgca attgtaagtg
     5941 aaggttgatt ggatttgaaa tagaaaaagt attttaacat gtttctgtag attgtataaa
     6001 aataaataaa tcaacaaagg agaatcaaag atgaataata aaaaaattgc agttatattt
     6061 ggtggaaact caacagaata tgatgtatca ttacaatcag cttttgcagt acttgagaat
     6121 ttgaatacgg aaaaatatga tattatacca attggaatta caagggaggg taattggtat
     6181 cattatattg gagattataa taatatccag aataacactt ggtttgaaag ggatactgat
     6241 ataattccag ttattgtttc gcagaaccgt gtcaaaaatg gtattattga atttttaatt
     6301 cctgatgctg tttctcagaa tcatattcca aatagtgcta ttgaatcatg tgaaaatgat
     6361 attaaataca ctaaacttga ttttgtattt cctatattgc atggaaaaaa tggagaagat
     6421 ggaacagtgc aggggttatt tgaattggca ggtatacagg ttattggatg tggtactctt
     6481 tcttcagcat tatgtatgga caaagacaaa tcacataaac ttgttcattc agaaggtatt
     6541 aaagtaccaa aatcaatatt actaaaatac tcagataaat ttaatgtggc tgaactgtca
     6601 aaagacttaa cttatccatt atttataaaa cctgtgcgtg caggttcatc atttggtatt
     6661 agtaaaattt ataaaaaaga agagttacaa gtagcagtag acttggcttt tgaacatgat
     6721 aatgaagtaa taattgagga aaatattgat ggttttgagg tagggtgtgc agttttagga
     6781 aacgatgact tacttgttgg aagagtagat gaaatagaat tgtcaaaagg attttttgat
     6841 tatacagaaa aatatacatt gaaaagttca aaaattcata tgccagctag aattgatttg
     6901 gattgtgaga aaagaataca agagacagca aaaattattt atagaactct tggatgttct
     6961 ggatttgcta gagtggatat gtttttaact ccatctggtg aaattatttt taatgaagtc
     7021 aatacaattc ctggctttac ttctcatagt cgttatccaa atatgatgaa aggtattgga
     7081 ctgtcttttg aagaaatatt ggacaagata attgaggtgt acataaaatg atgaataata
     7141 ttttattaga taaaaataaa gaagataaaa tgacaaatac tatcttattg actaaagata
     7201 aagtttatga tgggaatcta attctagtaa atgctttttt accagttaaa acatctgaag
     7261 atatagattt aattcctgta gatacaaggt ttccaagtat tcttatgaaa cgtgaagcta
     7321 caaacatttt acaaaatatt ttaaaaagta tatgtggaat aaatgaaata gtacctgtaa
     7381 gtggatatcg tactgcagag gaacaacaag atatatattc aagttctctg cgtgacaatg
     7441 gaaaagactt tactaaaaag tttgtagcac ttccaaatca cagtgaacat cagacagggc
     7501 ttgctattga tttagggtta aagaaagaag ttatagattt tatctgccct gaatttcctt
     7561 ataatggtat ttgtaatgag tttcgtaagg cagcactaca ttatggtttt atagagcgtt
     7621 atcagtgtaa aaaagaagaa ataacaggaa tatctcaaga accatggcat tttcgttatg
     7681 tgggatatcc gcattctgag attatcaata aaatgaatct cgctcttgaa gaatatatag
     7741 aatatattaa gagattttcg tatacaaatt gtttaaaaat agaagataaa gaaaaaatta
     7801 tagaaatctt ttatgtacct attcatacta aagaagaagt gagaatctct atacctgaac
     7861 aatgtgtata tcaaatatcg ggtaacaata aggatggttt tattgtgaca ttttggagaa
     7921 atagaaagga ctaagaaatg gaggaacaag aatgattagg aataaatctt atgcagggct
     7981 tgattatttt aggattatag ctgcattact tataattgca attcatacat ctccattatt
     8041 atcatatagt aaaatggctg actttgtgtt aactagtata attgctcgta ttgctgtgcc
     8101 attctttttt atgctatcag gcttttttct aatttcaaca tataattata attgtaataa
     8161 actgaaatct tttctaaaaa atgtattatt tatttacggg atagctattg taatttatat
     8221 tcccttaaat atttacactg gatatttttc catgaataat ttagtgccca atattattaa
     8281 ggacattgta tttgatggga ctttatatca tttatggtat ctacctgcat caattattgg
     8341 tgcaagtatt gcatactttc ttgtaaaaaa actaggttta aagtactcac tagtaatcac
     8401 tattgttatt tatgtaattg gtatgtttgg tgatagttat tatggttttt tagaaaaaat
     8461 accactatta aagaatttat atgggtatat ttttgaagta tcagattata cacgaaatgg
     8521 tatatttttt gcacctgtat ttttcatact tggtggaata atatctaata aatctaatta
     8581 tatgtcaact aaaaatagct tgattggatt tcttatatca ttcttactta tgttatgtga
     8641 gggaatgttt ttacataaac taggggtaca gcgtcatgat agcatgtata ttatgttatt
     8701 gccatgtatg tatttcttat ttacagcctt aacatttttg aaaggttctc gagctaggtt
     8761 attgcgaaca tcatcattaa ttatttatat tatacatcct atgatgattg ttgttattcg
     8821 tatgttttct aaaatattcg gaatacaaac tgtcttggtt gacaatagcc ttattcatta
     8881 tatagtggta agtatagcat cttttatagc ttctataatt attatattga ttcttaaacc
     8941 tctaaaaaaa gacttgaaag gagaacataa ggtaaatacc aatagagctt ggattgaaat
     9001 tgatttaaat aatttggaac ataatgtaaa gatgatacaa aaagctatgc cagaagattg
     9061 tgaattgatg gcagttgtaa aagctaatgc ctatggtcat agtgcttttg aagtagcgac
     9121 ttgtgcaaac caaataggtg taaaagcatt tgcagtagca acaatagatg aggggattga
     9181 tttacgtcga tatggaatac taggagaaat attgattctt ggatatacta acccaataag
     9241 ggcaaaggaa cttcataaat acaatcttac acagagtgtt attgacctta atcatgctat
     9301 ctctttaaac aaacagaaat acaacataaa atcacacatt aaaattgata caggtatgca
     9361 cagactgggt tttgatgtta atgatgtgag gtcaatttta aaggcattta aattaaaata
     9421 cttagatatg tgtggcatat ttacacactt gtgtgtttca gatagtctga aagatgaaga
     9481 tgtagacttt acaaataaac aaattgaatg tttttataag ttaattgatt ttctgttaaa
     9541 aaaaggtata aaaattccta aagtacatat tcaaagtagt tatggttttt ttaattatcc
     9601 caatcttaag tgtaattatg taagaattgg aattgcatta tacgggatat taagttctcc
     9661 aaatgatacg actaaattgc aacttgattt acgacctgtt ttatccttaa aatcacaagt
     9721 aattcttatt agaaaaatac aaaaagatga tagttttgga tatggaagag tctttattgc
     9781 aagcagagat agcgtaattg ctatattatc tattggctat gccgatggta tacctagaaa
     9841 tttatcctgt ggaaaaagtt acgtaataat aaatggttgt agagcagcaa tagtgggtcg
     9901 aatttgcatg gaccaacttg ctgttgatat tacagatata cctaatgtag aagttggtaa
     9961 tacggcaata ataataggaa gagataattt atctgaatta tctgcttctg aagttgcaaa
    10021 taactcatgt agtatatcga atgaattatt gagcagagta ggaagaaggt taaatgttat
    10081 aaagaagtaa ctcaattaaa tagtaatatt tgttgtactg tgtaatttat tttactctga
    10141 ctagacattt ttagtaatat tatgtctaat atatattttt tataacttac atgacaattt
    10201 aagttataaa aaggtgatgt ttcagatttt tattttgaaa catcaccttt tttgttataa
    10261 tatataataa aataattata cc
//
LOCUS       DAEORZ010000365         8130 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.guided.167, whole genome shotgun sequence.
ACCESSION   DAEORZ010000365 DAEORZ010000000
VERSION     DAEORZ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8130
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.guided.167"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(607..1422)
                     /locus_tag="KRH39_003945"
     CDS             complement(607..1422)
                     /locus_tag="KRH39_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG8513801.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLFFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHKDKLPKIT
                     PHTLRHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSARAEMERL"
     gene            1523..2221
                     /locus_tag="KRH39_003946"
     CDS             1523..2221
                     /locus_tag="KRH39_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8513802.1"
                     /translation="MYKLSYVVRVSTKDLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALVSQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSDDESKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            2309..2929
                     /locus_tag="KRH39_003947"
     CDS             2309..2929
                     /locus_tag="KRH39_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG8513803.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            3085..3867
                     /locus_tag="KRH39_003948"
     CDS             3085..3867
                     /locus_tag="KRH39_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBG8513804.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKKAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            3992..4918
                     /locus_tag="KRH39_003949"
     CDS             3992..4918
                     /locus_tag="KRH39_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513805.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKISVKQGNETVEIADG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            4932..5381
                     /locus_tag="KRH39_003950"
     CDS             4932..5381
                     /locus_tag="KRH39_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8513806.1"
                     /translation="MELIEWNYAYITELVEFANDKEIANNLRDNFPHPYREQNARDFI
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            5740..6147
                     /locus_tag="KRH39_003951"
     CDS             5740..6147
                     /locus_tag="KRH39_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8513807.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            6149..6379
                     /locus_tag="KRH39_003952"
     CDS             6149..6379
                     /locus_tag="KRH39_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8513808.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            6769..6972
                     /locus_tag="KRH39_003953"
     CDS             6769..6972
                     /locus_tag="KRH39_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG8513809.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            7050..7403
                     /locus_tag="KRH39_003954"
     CDS             7050..7403
                     /locus_tag="KRH39_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG8513810.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVDSFAKPQFVYSWK
                     LVSTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLHAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            7475..8107
                     /locus_tag="KRH39_003955"
     CDS             7475..8107
                     /locus_tag="KRH39_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG8513811.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         2894 a         1196 c         1394 g         2646 t
ORIGIN      
        1 cattaaaaat actatacatt ataaaaatac taatttaaaa ataattttta ttctaattct
       61 taaaacaatc ccacatattt tagaaaaggt actctataat atacaaacac ttctttttta
      121 cctaaattta ttttaacatc ctataatttt cacaatattc tttaaatttt actcatataa
      181 acaatattat tctggatgct aatagaatta atctataaat catagtataa ttatgcagaa
      241 ataactcgaa tttcactact tatgaagaga ttgaatatta atgaatctaa atcatttgca
      301 ttacttaaga gttttgacta aagttgaaca ccatacaaaa gtggctactc aattgtttat
      361 accccaacca agcttaagta atgttatata gattctcgaa aaagaatttg gtatcttttt
      421 atttgaaaaa cttatattaa atttgattat cttttcataa gttggtaaat gtcgatatat
      481 cctgtatcta caaggagtat cggcattttc tttttggaag atactttgca taagttggca
      541 tttaaccgta taagaatgcc atcaaaagta gtaaataagt agtaaactct agtcatacta
      601 ctaggcttat aaccgttcca tttcagctct cgcagaacta aatgaaccat gtgcataata
      661 gccaagcgtc atagtaatgt ttttatgccc cataatatat tgcaatatat taggactcat
      721 tcctttgtta gccatatttg tacagagtgt atgcctaagg gtatgtggcg taatctttgg
      781 tagcttgtcc ttatgggtct tgttatactt cttgactaac gctcttaaca tgctttgata
      841 atgagttgct tgttttggta atccatcttg tttaagaaat aggaagttct ggtaaccatc
      901 aacaatgatt ggttctgctt tagcgttctt acgccgttca acgatacgtt caaatgattg
      961 ataagctctt tcagtcattg gaatttctcg cttaccctgt gtggtctttg gaacttctac
     1021 ataatagcca atcttagtat cactcaacaa ttgatggtct accataatgg ctttattttt
     1081 catatccaat tctaaagtca aaccgcataa ttcagaaatg cgtagtcctg tttctaagag
     1141 gataacaaca tcatcataat atttacaata agtcttatcg ctctccataa agaataataa
     1201 gcccatttcc tgttctggtg ttagagcgat ttttggttct gttgtatctt ccaaaacatc
     1261 acttaactga aaattaaatg gattctttct aatacagtcg tcttgaattg ccatgaaaaa
     1321 cgacgctttc agtgagcgct tataattgtc aatggattta taagcacacc catcttcact
     1381 cattcggata gcccagcttt tagcgtctga taatttaacc atatcaatgc tttttgcacc
     1441 tagtgggtga ttgatagtat ctatattttg atttataatt tatacatttt tttaaaaaca
     1501 gacttttaac tgtaaagaat ctatgtataa acttagctat gttgtccgtg tatctacaaa
     1561 ggatcttgac gagctaaaaa ggcgttgtga tgaagtcaaa gacttttacg acgacttatc
     1621 aatcaagctc gtgcgtccct ttggggatat gttgggattg catggcgaat ttattcctgt
     1681 cagcaaacgt tacatcaatg attatattca atatgtcact tcggattttt tggctggact
     1741 tggttttgga gcgacacaaa cccttggcga acatgaggat atctatcttg gctacaatct
     1801 ggacactgga aagaatgtct atctaaaacc tgcccttgtt agtcaagggg taaagggttc
     1861 tattaccaat gcgttagcgt ctgcgtttat cggttcattg ggtggtggta aattattcag
     1921 taacaatatg cttgtttact atgcggtact attcggcgga caggcggtca tagttgaccc
     1981 taaagcagaa cgtggcaaat ggaaagaaac gctaccagaa atagcccatg agataaacat
     2041 tgtcaattta accagtgatg atgaaagcaa aggattgctt gacccttacg tcattttgag
     2101 ccgtaaaaag gattctgaaa gtttagcgat tgatatccta acattcctta ctggtatctc
     2161 cagtcgtgac agtgataagt ttttgcagaa aaatgacaac tggaaaataa taatggaata
     2221 gtttctatgt aaatatattt ttactatctt acctaactaa ttgaaagatg atataattta
     2281 atcaaagatt tggtaaggag ggatgaatat gcatttaatt gattttatga atgaagatat
     2341 taaaaagaag cttgttaaaa aaacgtataa aaagaaagag actattcttt ttgctgagca
     2401 agaaaatgaa tatgtaattc ttatgattga cggtattgca gaagcattca tcttaaatgt
     2461 gaaaggaaac atctcaacta ttcatcttta tagaagtggt agtttttttg gagaaatgga
     2521 acaatttaat gatgggaaaa aacccgttga aattgtcgct ttcacagatt gtaatgtata
     2581 taaacttcat agagatgatt ttttaaactg gttaagggga gattttgaag ctacaaaatt
     2641 tctgattcga gaaatatcta gtaaattagt tgtaaatgct gaattaattg aagaattatc
     2701 atctttaagt gttaaagaac gacttctgag gtgtatatcc ttacactatt atagaagtga
     2761 attaaatctg cttaccaaaa aacagcttgc tagcgaagta aacgctccaa ttagaagcat
     2821 taatcgtgct ataaatgaat gtgtacaaga acatctaatt gcttatgaaa acaaaagatt
     2881 tagtgtaatt aatcagcgag aggtattaaa gaatcttcct acaacataag tataatttca
     2941 aaaaagtatt gaataataat tattcaatgc ttttttattt cgggtcaagt gacctatttt
     3001 tttttatttc ttcattatat aatactccta tgcataatta tgttggattg atagctaata
     3061 aaagacagtg agaggtgtta tattatgact aaaacacaac agaaaatgat acaaaacgta
     3121 ttagaatttt caaaaagaga tagtaggata ataggacttg caattagcgg ttcttatatc
     3181 acaaaatcgt tagatgaata ttctgatttg gattttctga ttgtagcaga tgataaatcc
     3241 tatgaagaag ttatcgaaga gagattactt attgtggaac agtttggaac attagtttct
     3301 gcttttactg gcgaacacgt tggagaacct cgattgataa tttccctcta tgattcccct
     3361 gttcttcatg ttgattttaa gtttacaacc ttagatggtt tactagatag ggttgaagat
     3421 tcagctattc tctatgaaga aaacaactgc ataacagaaa tttattctaa aaaaaaagca
     3481 tacttcccaa ctccagactt acagtggatt gaagatagat tttggatttg ggttcattac
     3541 gcttgcacta agattggacg tagagaacta tttgaaacaa ttgatttttt atctttttta
     3601 agacaatcag tacttgcccc attaatacag cttctcttag gtaaattacc tcgtggagtt
     3661 agaaaaattg aattggacgc accccaatat attgaagcat tagaaaagac ggttgctaac
     3721 catgaatata aaagctgtgt taatgcattg caggctacta tcaacctgta tcttgagtta
     3781 agagaggaat tatcacaaag tcctattgtc cgaaaggttg aagctgaaag ggttgcatta
     3841 cagtattttc aagatttaat caaataaacc caaagtaaaa aaatattgaa tagtaaatat
     3901 tcagtgtttt ttcatttttg gtcaaatgac ctagattagt tgttgtaacc attctataat
     3961 tattttgaaa gtaacagtat ggaggataac tatgagtgat attttaagta gaattacaaa
     4021 taaaattggg gataagaata tagttgataa gttatcagct ctatcaaaat cagatttgaa
     4081 ctcacttcta ttagaagtgt ttgatagaca agcaaatact ttaactgcaa ctgatattct
     4141 aaagtcatac caactaaata gatttactat ccctagcggt atagatccag aagaaattca
     4201 tgctttggag tcaaaattat tgagaaaagc attcaatatg gatatcaaga cgattatgct
     4261 ttctccctct gctccattag gtagctgtag cgtttttggc tccgtagatc aatataatgt
     4321 agtaagtgct ctacgaggga cagaaacact tgcagacccc tcaaatatgt tggcaataat
     4381 tattgctgat aaattaaaaa gaaaagaaga aaataatacc atacctatcc acatggctac
     4441 tactgctagg gtaataagag cacaaaattt tgtaggtaaa ggtttatatt cacattttgg
     4501 cttatattgt atggtttcat ctggtataga tacagggtct tacacttgtg aaaaaatgtt
     4561 gttagaaaag cacttaaact attataaaga tatattaagt gaaatagaaa atgttcgttt
     4621 ctctattatt ctacgcaaaa gaaacggata caaagataat gacggatttt tcgacagaat
     4681 ggttgaaact atcaaactta tttttcctaa tatagagtta tcaattcata atgatgatgt
     4741 tgataataat tattataaag gtatcaattt caaaatatcg gtcaaacaag gaaatgaaac
     4801 cgttgaaata gctgacggtg gatttgttga ctggacttat caaatgcttg gaaataaaaa
     4861 agaacgctgt ttgataagtg gtattggctt agaaagattt cttgtagctg tttcataaaa
     4921 ggagaagaaa aatggaactt atagagtgga attatgctta tattaccgag ttagttgagt
     4981 ttgcgaatga taaagaaatt gcaaacaatc ttcgtgacaa cttcccccac ccttataggg
     5041 agcaaaacgc tagagatttt attagttttt gtttggcaac tccagatgtt aaacaactta
     5101 gctatgctat tttttatcaa gaaagggcta ttggaagtat cagcctaaca tttgaagaag
     5161 atacctctat taataaaaat gctgaattag gttactggct tgctaggaac tattggggca
     5221 aagggatagc aacaaatgca gtaaaagaaa tatgcaaaat tgcctttgaa aaatacaacc
     5281 ttaacaaaat tcatgctaat gtatattcct acaatagagg gtcaagtaaa gtattggaaa
     5341 aatgtggttt taagattgaa gaatactgtc acaattcata ataaagaata gaaacatgat
     5401 tgattgccat gtatatagtt taattcgacc gcaatagcat ttattagaat taacctactt
     5461 tttaataaca gaataagttt ttcccctcta ttaatttctt atgcatcttt gtatttttat
     5521 gaaaagctag ccaatgcata tttccataca tcaatatggt ggtggcttac aaccaatata
     5581 aattaaatac gttttaaaga atacacattt ttgccctata ggcagagtgt gtattttttt
     5641 ctcttttttt gaaatttatc gaagatttaa cgccctccca ccgtgatagg agtaagcaag
     5701 gttatttaac tccttgcgat ggaaagaggg tgaaatctta tggttgatga aaagacaatt
     5761 gaacatcaat tcgacagttt ctgtaaaaca gttttgcgta actatgctag agatatttat
     5821 gacgaaaata aacgcagaaa tgattatttg gtatctctcg aatctttgtc acttgcagaa
     5881 ttaagtaaat tgagtatttt agatgattat gattctaact acatctgtat ggtttcttac
     5941 gactacaaca tacgaattga agatgtatta atggcacaag caatcggaaa acttacaaaa
     6001 agaaaacaag atataattct actttcattc tttctcaaca tgacaaatgc tgacatagca
     6061 acattgatgg atttagcaga aaataccata cattatcaca aaacaaatgc actaaaagag
     6121 ttgaaagaac tcatggagga acattgaaat gaaacctact aaagaagtat taccttttac
     6181 aattatttta cttgctgtac atggcgatgt aaatgcaata aacggaatac ttaaacattt
     6241 tgaacactat atcattcgtc tttcacagaa aactttattt gatgaatacg gaaatcccta
     6301 tattcatgta gaagaagaaa caaagcgaat gctaggaaca aaactaatca ctgctatttt
     6361 gagatttaat ttgtcttaat ctcgccctct ctccatgtat caagctattt ggtacatgga
     6421 gaatcactaa ttatttgtct ataaaagccc acaagctatt gtgatttttt ataggcaaat
     6481 caatgccgac tgttctttga caactgaata aagtagatag atacgtttgt gatacagata
     6541 gccaacaaat actatacgcc atgaccttaa tcaagagagc tactatatag tgatttgtga
     6601 ataacctttg gaacggttgt tttgccgtaa cctgtaccac agtataatga tacacctgta
     6661 tgacacgatt ctgcccataa gaatgggatt ggttgaaata ccaatggagc ttaccaaagc
     6721 catctatcta cttctgaact acgaacatag aaaggagtga tacattctat gaacaatact
     6781 gatgtaccta tttgggaaaa atataccttg acgattgaag aagcgtcaaa atactttcgt
     6841 attggagaga aaaaattacg taaacttgct gatgaaaacc ttgattctgg ctgggtcatt
     6901 atgaatagca atcgcattca aataaagaga aaacaatttg agaaaatcgt tgatacactt
     6961 gacgttatct aataaaatcg agccttatat gttataatat tcatatgacg tatcaagctc
     7021 aattcggtta ttgagaggag cttcttatca tgtcaactga aaaaagacga gataataaag
     7081 atagaatttt aagaactgga gagagccaga gaaaagatgg aagatacgca tataagtatg
     7141 tagattcatt tgctaaacca caatttgttt attcttggaa acttgtatct actgataaac
     7201 ccccaaaggg aaaacgtgat gatatatcat taagggataa ggaaaaagca atacaaaatg
     7261 accttgatga tagcattgac actattggta agaaaatgac agtttgtcag ttacacgcta
     7321 aaaagaatgg actaagaaag aatatccgac ttaacactaa aaaaggacgc cattatctta
     7381 tgaagattct gaacaaggag tgattctgtg aagagatgta ttgtagtagt ggaatagtca
     7441 aaaacataat atatattaaa ggagagattt aaatatgact attccaagtt caaataaggt
     7501 atatccaaga agtaatgatt atcaaactgt atatcttaaa aatgtaataa caagggaaaa
     7561 tatagaagtt ggagattaca caatatataa tgacttttat aatgacccaa gagattttga
     7621 gaaaaacaat gtattgtatc attaccctat aaacaatgat aaattaataa ttggtaagtt
     7681 ttgctcaata gcatgtaatg caaaatttct aatgactagt ggaaatcact caatgaaatc
     7741 aaaatccaca tatacttttc ccatctttta tgaagaatgg aaactcgata tcaaccatat
     7801 aacggacgct tgggacaata agggtaacat tataattgga aatgatgtgt ggataggtta
     7861 tgattctata attatgtcag gtgtaaatat tggtgatggt gcaattattg gaacaagagc
     7921 agtagttact aatgacgtcc caccgtattc tattgttgga ggagtacctg caaaaattat
     7981 aaaaaagaga tttaataatg atacaatttc aaaattatta gaaattaaat ggtgggattg
     8041 gccatataaa aaaattcaat caaacattaa gcatattcaa tcaggaaata ttgataaatt
     8101 agtttaatat tttataattt atttatatta
//
LOCUS       DAEORZ010000366         5331 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.guided.209, whole genome shotgun sequence.
ACCESSION   DAEORZ010000366 DAEORZ010000000
VERSION     DAEORZ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.guided.209"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            1013..2275
                     /gene="tetA(P)"
                     /locus_tag="KRH39_003956"
     CDS             1013..2275
                     /gene="tetA(P)"
                     /locus_tag="KRH39_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter TetA(P)"
                     /protein_id="HBG8513812.1"
                     /translation="MVNKLSAYKTYLLFSAITAMCFSLVATVMIVYHIETVHLNPLQL
                     ILVGTTLEVACFIFEIPTGIVADVYSRKLSIVIGVVLTGVGFILEGSISSFVFVLVAQ
                     IVWGLGSTFISGSVEAWIAEEEKDKDLDKIYIKGAQAGQIGSVIGIVLSTVIANLSVR
                     LPIIVSGVLFIILALFLWLYMPENNFKSSAPEDLNTFKKMGYTFKSGLKFIKNKPIII
                     ILLSVTLFYGLSSEGYDRLSNMHFLQDTMLPKLGNLKPVTWFGIFGIAGMILSAIVMH
                     FMEKKLKDDDKNKNGKLLLCINIFYISFMFIFAITKRFNLMLIAYLATSTFRTINEPI
                     FSAWLNGHIDDKARSTVLSINGQINSLGQILGGPIIGIIATNISVSMGIACTSLLVTP
                     VLVLYIVAMIMDKKVVDRVGGIDYEENN"
     gene            2259..4217
                     /gene="tetB(P)"
                     /locus_tag="KRH39_003957"
     CDS             2259..4217
                     /gene="tetB(P)"
                     /locus_tag="KRH39_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein TetB(P)"
                     /protein_id="HBG8513813.1"
                     /translation="MKKIINIGIVAHVDAGKTTITENLLYYSGAIKSVGRVDLGNTQT
                     DSMELERKRGITIKSSTISFNWNNVKVNIVDTPGHVDFISEVERSLSVLDGAILVISG
                     VEGIQSQTRILFETLKELNIPTIIFVNKLDRIGANFNKVFEDIKKNMSNKVVRLQEVY
                     DVGSKAVYIKKLFDTCMINDDAIDVLSDLDEAFLERYIGGIEPDKEEIQEKLSLYASE
                     GSLYPVFCGAAAIGLGVEDLLDGICSYFPFAGDDCESDLSGVVFKIERTSKNEKKVYV
                     RLFGGKISVRDKIQVPNKEIAEKVKKINRLENGGVVEAQRIEAGDIGILYGLTSFQVG
                     DVIGISNDKIKNISIAKPALKTTISAIDKEKNPELFKALTLLAEEDPLLELEMNDIDK
                     EIYVNLFGEVQMEILSSMLDDLYGIKVEFSNIETIYKETPKGFGASIMHMQEDLNPFW
                     ATVGLEIEPAGRGEGLRYISNVSVGSLPKSFQNAIEEAVIKTSKQGLFGWEVTDVKVT
                     LSCGEFFSPASTPADFRNVTPMVFMEALYKAQTVLLEPLHEFELKIPQNALSKAVWDL
                     ETMRATFDNPIVIGDEFSIKGLIPVENSKEYKMKIASYTEGRGMFVTKFYGYKEASAE
                     FSKARKKTTYDPLNKKEYLLHKLNAIRD"
     gene            4408..4686
                     /locus_tag="KRH39_003958"
     CDS             4408..4686
                     /locus_tag="KRH39_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8513814.1"
                     /translation="MKIDFKITKDDYISFNLHHLENSKSQKSTFNILRYAVPIVLSIP
                     IYFTGTGIFNQPNIYWIIVAIVFLVIWILTYPKQYKKLVAKETDKLIS"
     gene            4702..4941
                     /locus_tag="KRH39_003959"
     CDS             4702..4941
                     /locus_tag="KRH39_003959"
                     /inference="COORDINATES: protein motif:HMM:NF025675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HBG8513815.1"
                     /translation="MFGSKTLEIIDGTIVVKGDSISETVSLESIKDVKVYEDMILIYI
                     SGFVAHIIPRRYLDKEIEKELMKELKKCKALEQLK"
     gene            5135..>5331
                     /locus_tag="KRH39_003960"
     CDS             5135..>5331
                     /locus_tag="KRH39_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8513816.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKA"
BASE COUNT         2045 a          552 c          965 g         1769 t
ORIGIN      
        1 attataggaa tagtattagt aatatttata ataagtaaaa taattgctgt taaaaaataa
       61 gatatggaat aaaaattaaa agtaaataaa attaactaat tcttgatgta gataatttag
      121 aacgctccgt agggtaaggt acggagaacc gtatcacaag taagtgcgaa ctttaccaaa
      181 attggatata taaatgaaag gtaggtctgc tttaattctg caatgaaact cacaaaaatc
      241 taaaatttga atttttgtag gttattgtag ttttattcat aggttcttcg tttttcacct
      301 atttcagcaa attagaattt gttttttcta atctatagta ttgaaagaaa ttgtaaaaca
      361 ttccacaaat atatttttta tattcatttc agatttaggt taaagttagt agttattctt
      421 ttgtgtatat aagttatttg aagtaataaa gtttcaattg aatttggata aatatgaata
      481 taaaagtatt gaaaaaaata aaagtttata tataatgtaa ttatactaaa atataaacat
      541 gaaaaatata aatgtaaagg agatgtaaat atgcgcaaag aggtagtttt taactaaact
      601 aactgaataa ggattatttt aaaaagtata tgaaaaaaat cttggataac tcaaggttta
      661 tttgtggtag aaacaattat taataatatt gatattacca tgtctatttg tgtactctaa
      721 aaataatcag tattataatg agcatattac atctttattt gaagaatagg aaaactgtgt
      781 tctaaacacg gtatttttat gcctattttt actatattta tagatatgat ataagacttt
      841 aatttgcata gcttaatcat aagttatata aattaaagtt gtatatttca tgtttatatt
      901 taggttatat aatttttatt taaggagatt attttgatga tttatatatt taaatatgaa
      961 gattatgaaa aacaaataaa tttttttaaa aatactagga tggtgattat taatggttaa
     1021 taaactttca gcatataaaa cttatttatt attttcagct attacagcaa tgtgtttttc
     1081 actagtagct acagttatga tagtgtatca cattgaaacg gtgcatttaa atccacttca
     1141 gcttatactt gttggaacta ctttagaagt agcatgcttt atatttgaaa ttcctacagg
     1201 aatagttgca gatgtgtaca gtcgtaaact atctattgtt attggtgtag ttttaacagg
     1261 agtgggattt attttagaag gttctatttc tagtttcgtt ttcgtacttg tagcacagat
     1321 tgtatgggga ttaggttcta cttttattag tggatctgtt gaagcttgga ttgcggaaga
     1381 agagaaagat aaagatttgg ataaaattta tataaaggga gcacaagcag gacagatagg
     1441 atcggttatt ggaatagtac taagcactgt aatagctaat ttatctgtaa gactacctat
     1501 tatagttagt ggagtcttat ttataattct tgcattattt ttatggttat atatgccaga
     1561 aaataatttt aaatcatctg ctccagagga tttaaatact tttaaaaaga tgggatatac
     1621 ctttaaatct ggtcttaaat ttataaaaaa taaacctata attataattt tgctttcagt
     1681 aactttattt tatggattat ccagtgaagg ttatgataga ctttctaata tgcatttttt
     1741 acaagatact atgcttccta aacttggaaa ccttaaacca gtgacttggt tcggaatttt
     1801 tggaattgca ggaatgatat tgagtgctat agtaatgcat tttatggaaa agaagcttaa
     1861 ggatgatgat aagaataaaa atggaaagct gttattatgt ataaatatat tttatatatc
     1921 atttatgttc atatttgcta ttacaaaaag atttaactta atgctaatag cttatttagc
     1981 gacaagtacc tttagaacta taaatgaacc tatatttagt gcatggctta atggacatat
     2041 agatgacaag gccagatcta ctgtactttc tataaatgga caaataaatt ccttaggtca
     2101 aattttaggt ggaccaatta taggaatcat agctacaaat atttcagtga gtatgggtat
     2161 agcatgtact tcgttattag taacaccggt attagtgtta tatattgttg ctatgataat
     2221 ggataaaaag gtggttgata gagttggagg tattgattat gaagaaaata attaatatag
     2281 gaatcgtagc acacgtggat gcaggaaaaa caactataac agaaaactta ttatattata
     2341 gtggagctat aaaatcagtt ggaagagttg atttaggcaa tacacagacg gattctatgg
     2401 agcttgagcg taagagaggg attaccatta aatcatcaac catatctttt aattggaata
     2461 atgtcaaggt taatattgtt gatactccag gacatgtgga ttttatttcg gaagttgaac
     2521 gttcattaag tgttttagat ggagcgatac tagttatatc aggagtagag ggtattcagt
     2581 cacaaacaag aatattattt gagacattaa aggagttaaa cattccaaca ataatttttg
     2641 taaataagct agatagaatt ggggcaaatt tcaataaagt atttgaagat ataaagaaga
     2701 atatgtccaa taaagtagtt agattacaag aagtatatga tgtaggaagc aaagctgttt
     2761 atataaaaaa actatttgat acatgcatga taaatgatga tgctattgat gttttatcag
     2821 acttagacga agcattttta gaaagatata ttggtggaat agaacctgat aaagaagaaa
     2881 tacaagaaaa gctttcatta tatgcaagtg aaggaagtct atatccagta ttttgtggtg
     2941 cggcagcaat tggacttgga gttgaagact tattagatgg aatttgtagt tattttccat
     3001 ttgcaggtga tgattgtgaa agtgatttat ctggagtagt atttaaaatc gaaagaacaa
     3061 gtaaaaatga aaagaaggtt tatgtaagat tatttggagg aaaaatatct gtaagagata
     3121 aaattcaagt acctaataag gagatagcag aaaaagtaaa gaaaattaat aggttagaaa
     3181 atgggggagt tgttgaagca cagaggatag aagcagggga tataggtatt ttatatggac
     3241 ttacaagttt ccaagtggga gatgttattg gaatttcaaa tgataaaatt aaaaatatat
     3301 ctatagctaa accagcatta aaaacaacaa tttctgcaat tgataaagaa aaaaatccag
     3361 agctatttaa agcattaaca ttacttgcag aggaagatcc actactagaa ttagagatga
     3421 atgacataga taaagaaatt tatgtcaact tattcggtga agttcaaatg gaaatactaa
     3481 gttccatgtt agatgattta tatggaataa aagtagagtt ttcgaatatt gagactatct
     3541 ataaggaaac acctaaaggt tttggagcgt caataatgca tatgcaggaa gacttaaatc
     3601 cattttgggc gacagtaggc ttagaaatag aaccagcagg gagaggcgaa ggtcttaggt
     3661 atatttctaa tgtttcagta gggtcattgc caaaatcttt tcaaaatgca attgaagaag
     3721 cagttattaa gacaagtaaa caaggattat ttggatggga ggttacagat gtaaaagtca
     3781 ctcttagctg tggtgaattt tttagtccag ccagcactcc agcagatttt agaaatgtga
     3841 cacctatggt attcatggaa gcattatata aagcacaaac tgttttatta gagccattac
     3901 atgagtttga gttaaagatt cctcaaaatg ctttaagcaa agcggtatgg gatttagaaa
     3961 ctatgagggc aacctttgat aatcctattg ttatagggga tgaattctca ataaagggat
     4021 taattccagt agaaaattca aaagaatata aaatgaaaat agcttcatat acagaaggta
     4081 gaggaatgtt tgtgacaaaa ttttatgggt ataaggaagc ttcagctgaa ttttcaaaag
     4141 cacgcaaaaa aacaacgtat gatccattga ataaaaaaga gtatttgctt cataaactaa
     4201 acgcaattag agattaaatt taaatagata agttaaattt ttatatgtag gatttaaata
     4261 taagacattc aaagcctaaa agtaaaggaa gggtcgtctg aagaagatga agacccttcc
     4321 ttttttcatg ttactatgct taattgtaag tgcttgaaat ttgtaatata atgtaaataa
     4381 agtttttaat aataagggga cgcacaaatg aaaatcgact ttaaaataac aaaagatgac
     4441 tatataagct tcaatttaca tcatttagaa aactcgaaat cacagaaaag tacatttaac
     4501 atacttagat atgctgtacc catagtactt tctataccaa tctactttac aggaacgggg
     4561 atatttaatc aaccgaatat atattggatt atagtagcga tagttttttt agtcatttgg
     4621 attttaacat atcctaagca atacaaaaaa ttagtagcta aagaaactga taaactcata
     4681 agttaaggag ataattcatc aatatttgga agtaagactt tagaaattat tgatgggaca
     4741 atagtagtaa aaggggattc tatatcagaa acagtatcgt tagaaagtat aaaagatgta
     4801 aaagtttacg aagatatgat ccttatatat ataagtggat ttgtagctca tataattcca
     4861 aggagatatt tagataaaga aatagagaaa gaacttatga aagagcttaa aaaatgtaag
     4921 gctttagaac aattaaagta aaacgtgctc ctttggaggt gtaacggaga accgtatcat
     4981 aaataaatga tacggttttt gttagtttgc aacttaataa tattaaaata acaaaaaagt
     5041 attgaaagta tattgcattt aaggtagaat taaataaaat attataacat tataatgttt
     5101 atatagttgt taataaaaat ttggaggaaa atatatgata aaaataatgt tggcctgttc
     5161 agcaggaatg tctacaagtt tattagttac taaaatggaa agtgcagcta aagaaaatgg
     5221 tatagagtcg caaatttggg caataccaga atcaactata caaaatgaaa tagaaaaatg
     5281 tgatgtatta ttactaggac cacaagttag atatgtatta cctaaggctc a
//
LOCUS       DAEORZ010000367         5244 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.guided.212, whole genome shotgun sequence.
ACCESSION   DAEORZ010000367 DAEORZ010000000
VERSION     DAEORZ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.guided.212"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..234
                     /locus_tag="KRH39_003961"
     CDS             <1..234
                     /locus_tag="KRH39_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG8513817.1"
                     /translation="LCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITG
                     TAYEPWHIRYVGKDAAEEIYSKGLTLEEYLQNK"
     gene            368..1060
                     /locus_tag="KRH39_003962"
     CDS             368..1060
                     /locus_tag="KRH39_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8513818.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKN
                     ENIHLMVLDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFNGANNEDKDVIEVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            1050..2231
                     /locus_tag="KRH39_003963"
     CDS             1050..2231
                     /locus_tag="KRH39_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG8513819.1"
                     /translation="MKNKSLLNHMFRPILDLIISCGLTSMLILTSMYIIKLLYFNSEI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHNGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            2513..2968
                     /locus_tag="KRH39_003964"
     CDS             2513..2968
                     /locus_tag="KRH39_003964"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBG8513820.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAVECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            2958..3683
                     /locus_tag="KRH39_003965"
     CDS             2958..3683
                     /locus_tag="KRH39_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBG8513821.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNIVELEGTNLTLESKDIYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            3655..>5244
                     /locus_tag="KRH39_003966"
     CDS             3655..>5244
                     /locus_tag="KRH39_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBG8513822.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDECVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLA"
BASE COUNT         2145 a          514 c          991 g         1594 t
ORIGIN      
        1 ctttgtattg atttaacaaa tccagaaaga tggtttgttg gaagtacaaa agaagctaaa
       61 tggttggcag aaaatgctca caagtttgga tttataataa gatatcctga aggtaaagaa
      121 gatataacag gtacagcata tgaaccatgg catattagat atgttggtaa ggatgctgcc
      181 gaggaaattt attcaaaagg attaacttta gaagaatatt tacaaaataa ataatataaa
      241 tgtgtaaata atttgtatta tattggaaat ttagaaatga ataaatttat agattacact
      301 aactaaaata tagtataatt ttatatgagt aaagttatat acaaagatga agttaggatg
      361 tgtaaaaatg aacaatacag ttcttgttgt ggatgatgaa aaggaaataa gagaccttat
      421 agagatatat cttttaaata gtggatataa tgtaattaaa gctgaagatg gaaaagaggc
      481 tctagatatt ttaaaaaatg aaaatattca tttaatggta ttggatataa tgatgcctaa
      541 aatggatggg atagaagttt gtagaaaagt tagagaaagt ttgaatatac caatacttat
      601 gttaagtgca aaatcagaag acatggataa aatacaaggg ataatgactg gtgcagatga
      661 ttatttgaca aaaccattta acccacttga acttactgtg agagtgaaat cactgattag
      721 aagagcatat tattttaatg gtgctaataa tgaagataaa gatgtcatag aagttggagt
      781 agtaattata gataaggaaa aacatagtgt aacagtagct ggaaatgaaa taattttgac
      841 atcaagagaa tttgatatat tatatttact agcaaacaat aaaggcagag tatatagtac
      901 agaagaaata tttgaaaagg tatggaaaga gaaatattac caatctaata atactgtaat
      961 ggttcatatg agcagaataa gggataaaat tgaaaaatat acagatggag aaaaaataat
     1021 tcatactgta tggggagtag gatataaaat tgaaaaataa aagtttatta aatcatatgt
     1081 ttcgacctat attagatctt ataataagct gtggtttaac ttcaatgctt attttaacaa
     1141 gtatgtatat aataaaatta ttatatttca atagtgagat atctgtaatt aatcaacttg
     1201 tatatcagtt aagagatttc agaagtctta taggagagag tttattttat ccatttttat
     1261 ttacaatcac atcattagca atttattcca tgatatcgta caagagaaat aagcaaattg
     1321 tagattttat aagaaagact aatagaaaaa atcaaaaatt agaaatcatt aaaagtgctg
     1381 taaatttaat ggatgaaggt aacttagaaa aaagtataga tattgagtat gacttagata
     1441 tattaaagga taaagaaaag gatgatattg atgaattagc acataaaatc aataatatag
     1501 tccatcaact tagaaatata actattgaag agagacaagc tcagcagaca aaaacagatt
     1561 taataacaaa tgtgtcacat gacttaagga cacctttgac atccataatg ggttatttag
     1621 gagtgataga agaaggtaag tataaagatg aagttcagat gatgtattat gttgatattg
     1681 catatgaaaa atcgaaaaat ttaaatgttc ttataaatga tttgtttgaa ttgactaaaa
     1741 tgcaaaataa tacaataaaa ttaaacaaaa tagaaataaa tttagtagag ttattaagtc
     1801 aagtagtatc tcaatttgaa atttatttta aacaagaatt tatggttagt agaattaatt
     1861 ttaatgaaga aaaattaatt gtaaaagctg atccaaataa attggtaaga gcatttgaaa
     1921 atcttataac taatgctgtg aaatatggaa aagatggtca ttatgtagat atagtcacag
     1981 agaaaaagga agatatggcg gttgtaaaag tgataaatta tggagaacca attcctgtgt
     2041 tagatttacc aaatatattt gatagatttt atagagttga aaaatctaga aatagaaatg
     2101 ttggaggttc tggattgggt cttgcgatta caaaaaatat tgtaaattta cataatggag
     2161 aaattactgt ttcaagcgat aaatatcaaa ctgtatttga ggtacaactg ccaataatac
     2221 aatcagctta gttataaaat aaaagtacga ttatgtgata taaatattat aacaataaag
     2281 tgtttacaaa aataaaaaag taatatataa tttaagtcag tttaataaga tacttatcaa
     2341 gagttagggg agagagttag ctcagtgatc ctataccaac cttttatcag gtggtcctgc
     2401 taacaacgat gagatgactt gaaataatat actcttctta tcaataatag ataggaagag
     2461 ttttttttag aattaatgta acaaatttta aagtataatg gaggattaaa taatggaaaa
     2521 agtagaaagt tttaagctag accatacaaa agtaaaagct ccttttgtaa gaaagtgtag
     2581 tgtacttgat ggagtaaagg gagataaagt aactaaattt gatttgagat ttttacagcc
     2641 aaatgtagaa agttttggaa ctgctgctat gcacggttta gagcatcttt tagctactta
     2701 tttaagagat acattagatg gtgtaataga cttatctcca atgggatgta gaacaggatt
     2761 ttatttaata ctatggggag atgtagatgc caaaacagtt aagattggtt tagaagaagc
     2821 attaaaaaaa gttcttgaat cagataaaat gccagcagct acagctgtag aatgtggaaa
     2881 ctacagagac ttatctttat ttggggcaaa ggaatatgca aaagatgttt tagataaagg
     2941 attttcttta aatatatatg gagaataatt taattggaag agaagatata agcataaatc
     3001 aaagtgaagt tttaagatat ttacaataca gaggtcaaga gatagataaa gaccttaaat
     3061 atactattga tgaatgtgta gctataacta aaaataaaat aaatcctaga tatttatcta
     3121 ggatttatcc tattagaata aataaagata atattgtaga attagaggga acaaacttaa
     3181 ccttagagag taaagatata tatgaacttt taaaagaatg taatgagtgt gtaattatgg
     3241 cagctactat agggatagat atagaaaaag aaataagacg acactcttat tcaaatttaa
     3301 caaaaggact aataattgat tcatgtggaa caacagcaat agaagaagtg tgtgacattg
     3361 ttcaaaataa tttagaaaag gtttttttaa agcaagataa atttataact atgcgatata
     3421 gcccaggata tggagattta ccaatagaaa aaaatataga tatattaaac gttttgaatg
     3481 ctcaaaaaca aataggtcta acaattactt ctaatggaat tatgataccg agaaagtcag
     3541 tagtggctat tataggtatt tctaatacaa gaataaataa atctaagaaa gaaaagtcgt
     3601 gtgaaaattg taaaaactat agaaactgta catatagaaa aggagcgaac tcttgtggaa
     3661 ataagacaat atctgaaaaa taatatatta atatttgatg gtgcaatggg aacaatgctt
     3721 caacaaaaag ggttaaagtt aggtgaaaat ccagaagtat ttggtcttca aaatccagat
     3781 aaacttatag aaatacatac agcatactta gaggcaggtt caaatgtaat tcttacaaat
     3841 acttttggat gtaatgaact aaaattagat agtaagtata ctgtagaaga agtgattgac
     3901 aatgctgttt tggtggctag aaaagctata gaaaatgtag ataatactaa accaagatat
     3961 gtagctttag atattggtcc tataggagaa atgctagagc ctatgggtac tttaagtttt
     4021 gataaggcat atgaaatatt taaaagacaa gtactacaag gcgttaagtc tggagttgat
     4081 gtgatagtaa tagagactat gatggattta tatgaagcta aagtagctgt gttagcagct
     4141 aaagaaaatt ctgatttacc tattttctgt actatgacat ttgatgaagg tggaagaagt
     4201 tttactggat gtatgccaga atgtatggtg gctactatag aaggtctagg tgtagatgca
     4261 ataggagtta attgttcatt aggacccaaa caacttttac caatagttga aaaaatagca
     4321 tcaagagcta cagtccctgt tatggttcaa gcaaatgcag gtcttccaaa tatagttgat
     4381 ggagaggcta tatatgatgt tgatgcaaaa gaattttttg aaggggttaa aaagttcgtt
     4441 gaggttggtg ctactataat aggtggatgt tgtggtacga atccaagttt tataaaagaa
     4501 atatctgaga atataaattc tgttacaaag ggatgtatcg agaaaattga tgaatgtgta
     4561 gtctgttcac catcaaaatt tgtggaagta caatcgccta cagttgttgg agaaagattg
     4621 aatcccacag gtagaaaatc tctacaagaa gcattaaaaa atgaaaatgt tgattatgct
     4681 ataaatttag gcttagaaca agtaaatgct ggagcacaaa tacttggagt gaatgttggt
     4741 cttccagaga ttgatgaaaa aaaactaatg ccaaaattaa taagagagat tcaagcagta
     4801 gtagatacac ctttacaagt tgattcttcc aatgtagagg ccttagagca aggacttaga
     4861 tattataatg gtagaacaat agtaaactct gtaaatggca aagaggagtc cttagaaagc
     4921 atattaccaa tagttaaaaa gtatggttca tgtgtggttg gacttacact tgatgaaaag
     4981 gggataccta aaaaggctga agaaagattt gaaatagcaa aaaggatagt taatagagca
     5041 gtttcttatg ggataaaacc aaaagacata tttatagatt gtttatcatt aactgtttca
     5101 gcacaacaag aagaagcaat agaaactata aaagctataa caatggtaaa gacactagga
     5161 gtaaaaacta tattaggtgt ttcaaatatt tcatttggtc ttccaaatag gaaagcactt
     5221 aatgcttctt ttctaacatt agct
//
LOCUS       DAEORZ010000368         1423 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01022
            SAMEA5849369-rid12486933.guided.314, whole genome shotgun sequence.
ACCESSION   DAEORZ010000368 DAEORZ010000000
VERSION     DAEORZ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849369
            Sequence Read Archive: ERR3473860
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070408.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070408.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,967
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,888
            CDSs (with protein)               :: 3,888
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 8
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849369-rid12486933.guided.314"
                     /isolate="CD-15-01022"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="KRH39_003967"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="KRH39_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG8513823.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYSIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKCVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEVAINIIKKYYSVRE"
BASE COUNT          606 a          114 c          236 g          467 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacctaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaataa taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat agtatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtactacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atgtaaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaatgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaagttg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ata
//
